Moisture Absorption Test
Aqua Gel Sheet

Cubic

Hygroscopicity

Hygroscopicity

105

25℃60%RH 4sheets(tray opened)

100

37℃80%RH 4sheets(tray unopened)

95
90

110

25℃60%RH 4sheets(tray unopened)

Relative value

Relative value

110

200

400

Time [h]600

40℃ 75%RH 4pcs

100
95

37℃80%RH 4sheets(tray opened)

025℃ 60%RH 4pcs

105

90

800
According to in-house data

0200

400

Time [h]600800
According to in-house data

Testing Method

Testing Methodroom humidity levels to determine the change in weight over time.

dishes, left for 4 weeks in environment testing machines at diﬀerent

KOKEN Perfect Aqua Gel Sheet was tested at diﬀerent temperatures andUnder each set of conditions, 4 sheets were left in the dedicated tray

(balance dish) with the lid open and 4 were left in an unopened tray.

They were then allowed to stand for 4 weeks inside an environmental
testing machine.

Four cubes of Cubic Koken Aqua Gel were placed on open balance

humidities, and temperatures,and their changes in weight measured.

Results

No clear change was observed under any of the conditions or environments.

Results

No clear change in weight was observed under any conditions orenvironment.

Package

Package

50sets/box

Cubic Koken Aqua Gel (475pcs)

Bottle50sets/box

Using freeze drying technology, collagen is successfully sealed inside
the sheet and kept freshContains naturally derived moisturizing
ingredientsSize
8(L)x8(W)x8(H)mm
Weight 10mg

Custom Orders (ingredients)

Custom Orders (outer packaging)

Coloring with natural pigments

Bulk delivery

Added ingredients

Label attachment

Aqua Gel Sheet & Cubic

Safety evaluation

Characteristics(Patch tested, cumulative irritation tested, allergy tested)

Can also be used with your favorite skin toner

Also handy for air travel where diﬃcult tobring cosmetics with you.

© 2021 KOKEN CO., LTD. C-1-806-13-1-01-00

1-4-14 Koraku, Bunkyo-ku, Tokyo 112-0004, Japan Tel: 81-3-3816-3562 Fax: 81-3-3816-3582 E-mail:customer@kokenmpc.co.jp

Aqua Gel Sheet & Cubic

Aqua Gel Sheet

Freeze Drying Technology

Number ofsheets to use

Heat stability of triple-helical collagen

Relative value where water holding capacityof 1 g of hydrolyzed collagen equals 1

Water holding capacity of triple-helical collagenSpeciﬁc rotation

Maintains triple-helical structure

Freeze dried storage
Storage in solution

Gelatinizes

151050Hydrolyzed
collagenGelatin

Triple-helicalcollagen

According toin-house data

According to in-house data

Storage temperature

Testing Method

Testing Method

Triple-helical collagen in 0.1% solution and triple-helical collagen freeze driedat a concentration of 0.1% were stored for 1 hour at temperatures of 25ºC, 30ºC,
and 40ºC, and their speciﬁc rotation was measured with a polarimeter (DIP-370).

Each ingredient was placed on ﬁlter paper in powdered form, and water
was dropped onto it a little at a time. The addition of water was stopped
at the stage where the substance could no longer hold water and thewater began to drip through the ﬁlter. The amount of water added before
the drip-through point was reached was termed the water holding capacity.

Results

Freeze drying technology prevented gelatinization of triple-helical collagenby heat, maintaining a triple-helical collagen structure with a highly
moisturizing eﬀect.

Results

Triple-helical collagen was found to have 13 times the water holding
capacity of hydrolyzed collagen (per gram).

We recommend trying it together with a facial massage forthose who want to understand how it really feels

Aqua Gel Sheet

Moisturizing eﬀect

Moisture Retention Test
Testing Method

Water content ofstratum corneum

40.0

With applicationof product30.0

Without applicationof product20.0

Results

10.0
0.00 weeks

After 1 weeks

After 2 weeks
According to in-house data

Change in wrinkle grade over time
With applicationof product*For one cube, use around 0.5mL of skin toner.

Mix and apply to your skin using
a gentle circular motion.

Squeezing the cube willcause it to be less soluble.
Magniﬁed image by electron microscope(According to in-house data)

Wrinkle grade

3.6

Without applicationof product

3.4
3.2
3
2.8
2.6

0 weeks

After 4 weeks

After 8 weeks

Maximum depth of largest wrinkle（μm)

Change in maximum depth of largest wrinkle over time

3.8

Tap the gel with your ﬁnger tip
after allowing the skin to absorb itwell.

Compared to the group that did not receive the application, the group to which
the product was applied showed an increase in moisturizing eﬀect after 1 week ofuse and a signiﬁcant increase at a 5% level after 2 weeks of use.

Test to evaluate anti-wrinkle function (Guidelines for the Evaluation of Anti-Wrinkle Products)

Cubic

Drop an appropriate amount ofwater onto the cube.

One KOKEN Perfect Aqua Gel Sheet was dissolved in about 0.5 mL of puriﬁed
water and applied to the inner part of one upper arm of 6 women twice daily(morning and night). Nothing was applied to the other arm. The water contentof the stratum corneum was measured with a measuring instrument(Corneometer CM825) after 1 week of use and after 2 weeks of use.

2520With applicationof product

1510Without applicationof product50-5-10-15-20-25

According to in-house data

After 4 weeks

Testing Method

Testing Method

ResultsResults

One KOKEN Perfect Aqua Gel Sheet was dissolved in about 0.5 mL of tap water andapplied to half of the face of 16 women twice daily (morning and night). Nothing
was applied to the other half of the face. The wrinkle grade evaluator assigned
scores on the basis of visual examination and photographs (EOS 5D Mark III) after
4 weeks of use and after 8 weeks of use.
Compared to the group that did not receive the application, the group to which
the product was applied showed a decrease in wrinkle grade after 4 weeks of use
and a signiﬁcant decrease at a 5% level after 8 weeks after use.

After 8 weeks

According to in-house data

One KOKEN Perfect Aqua Gel Sheet was dissolved in about 0.5 mL of tap water and applied
to half of the face of 16 women twice daily (morning and night). Nothing was applied tothe other half of the face. Skin replicas (Silﬂo) were taken after 4 weeks and again after
8 weeks, and the maximum depth of the largest wrinkle was measured with the use of areﬂective replica analysis system (ASA-03RXD) and 3-dimensional skin analysis software.
Compared to the group that did not receive the application, the group to which theproduct was applied showed a decrease in the maximum depth of the largest wrinkle
after 4 weeks of use and a signiﬁcant decrease at a 5% level after 8 weeks of use.