SaraFluor TM Series from GORYO Chemical
SaraFluor
CAT No.

ST1003-10
ST1003-11
ST1003-21
ST1003-31
ST1003-41
ST1006-10
ST1006-11
ST1006-21
ST1006-31
ST1006-41
ST1008-10
ST1008-11
ST1008-21
ST1008-31
ST1008-41
ST1010-10
ST1010-11
ST1010-21
ST1010-31
ST1010-41
ST1011-10
ST1011-11
ST1011-21
ST1011-31
ST1011-41

New product name

5 nmol x 5

SaraFluor 488-NHS
SaraFluor 488-maleimide
SaraFluor 488-COOH
SaraFluor 488-NH2
SaraFluor 600-NHS
SaraFluor 600-NHS
SaraFluor 600-maleimide
SaraFluor 600-COOH
SaraFluor 600-NH2SaraFluor 650-NHS
SaraFluor 650-NHS
SaraFluor 650-maleimide
SaraFluor 650-COOH
SaraFluor 650-NH2
SaraFluor 700-NHS
SaraFluor 700-NHS
SaraFluor 700-maleimide
SaraFluor 700-COOH
SaraFluor 700-NH2
SaraFluor 720-NHS
SaraFluor 720-NHS
SaraFluor 700-maleimide
SaraFluor 700-COOH
SaraFluor 700-NH2

STELLAFluor 488 NHS
STELLAFluor 488 maleimide
STELLAFluor 488 free COOH
STELLAFluor 488 free NH2
STELLAFluor 600 NHS
STELLAFluor 600 NHS
STELLAFluor 600 maleimide
STELLAFluor 600 free COOH
STELLAFluor 600 free NH2STELLAFluor 650 NHS
STELLAFluor 650 NHS
STELLAFluor 650 maleimide
STELLAFluor 650 free COOH
STELLAFluor 650 free NH2
STELLAFluor 700 NHS
STELLAFluor 700 NHS
STELLAFluor 700 maleimide
STELLAFluor 700 free COOH
STELLAFluor 700 free NH2
STELLAFluor 720 NHS
STELLAFluor 720 NHS
STELLAFluor 720 maleimide
STELLAFluor 720 free COOH
STELLAFluor 720 free NH2

1 mg
1 mg
1 mg
1 mg
5 nmol x 51 mg
1 mg
1 mg
1 mg
5 nmol x 51 mg
1 mg
1 mg
1 mg
5 nmol x 51 mg
1 mg
1 mg
1 mg
5 nmol x 51 mg
1 mg
1 mg1 mg

CAT No.

A202-01
A203-01
A204-01
A208-01
A209-01
A218-01

Size

STELLAFluor 488 NHS

SaraFluor B

A201-01
A201-02

Old product nameSaraFluor 488-NHS

New product name

Old product nameProteins such as antibodies can be labeled simply by mixing

SB

Probes blinking spontaneously for super-resolution imaging

B

Available for single molecule imaging with HaloTag® technology

TSize

HMSiR-Halo

15 nmol

HaloTag® SaraFluor 650B Ligand
SaraFluor 650B goat anti-mouse IgG
SaraFluor 650B goat anti-rat IgG
SaraFluor 650B goat anti-rabbit IgGSaraFluor 650B-NHS
SaraFluor 650B-maleimide
SaraFluor 488B-NHS

HMSiR-Halo

30 nmol
100 μg100 μg100 μg100 μg100 μg100 μgHMSiR labeled Goat anti-mouse IgG (whole)
HMSiR labeled Goat anti-rat IgG (whole)
HMSiR labeled Goat anti-rabbit IgG (whole)

HMSiR-NHS
HMSiR-Maleimide
New productSaraFluor T

A308-01
A308-02

SaraFluorTM Series※We also have large capacity products (5 mg, 10 mg), so please do not hesitate to contact us.

HaloTag® SaraFluor 650B Ligand

CAT No.

Fluorophores for Protein Labeling

New product nameHaloTag SaraFluor 650T Ligand
HaloTag® SaraFluor 650T Ligand®

Old product nameHaloTag® STELLA Fluor 650 Ligand
HaloTag® STELLA Fluor 650 Ligand

Size

30 nmol
60 nmol

References

GORYO Chemical’s fluorophores for labeling, STELLA Fluor Series and Super Fluor Series were integrated
into SaraFluor Series and renamed. "Sara" means "bright" in the native Ainu of Hokkaido. Your wishes are tobe such pigment that lightens your research brightly.
nuclear membrane
SaraFluor 488

mitochondria
SaraFluor 600

microtubule
SaraFluor 700

mergedSaraFluor B※ SaraFluorTM B corresponds to HMSiR in the references.
S. Uno, M. Kamiya, A. Morozumi, Y. Urano (2018) Chem. Commun. 54:102-105 DOI: 10.1039/c7cc07783a
F-C. Chien, C-Y, Linb, G. Abrigo (2018) Phys. Chem. Chem. Phys. 20:27245-27255 DOI:10.1039/C8CP02942C
S. Uno, M. Kamiya, T. Yoshihara, K. Sugawara, K. Okabe, M. C. Tarhan, H. Fujita, T. Funatsu, Y. Okada, S. Tobita, Y. Urano (2014) Nat. Chem.
6:681-689 DOI: 10.1038/NCHEM.2002
SaraFluor T※ SaraFluorTM 650T corresponds to STELLA Fluor 650 (SF650) in the references.
M. Yanagawa, M. Hiroshima, Y. Togashi, M. Abe, T. Yamashita, Y. Shichida, M. Murata, M. Ueda, Y. Sako (2018) Sci. Signal. 11: eaao1917
DOI:10.1126/scisignal.aao1917
G. Lukinavičius, K. Umezawa, N. Olivier, A. Honigmann, G. Yang, T. Plass, V. Mueller, L. Reymond, I. R. Corrêa Jr., Z. G. Luo, C. Schultz, E. A.
Lemke, P. Heppenstall, C. Eggeling, S. Manley, K. Johnsson (2013) Nat. Chem. 5: 132-139 DOI:10.1038/nchem.1546

Multicolor imaging of fixed HeLa cell using SaraFluor labeled antibodyDistributor

GORYO Chemical, Inc.
EAREE Bldg. 5F Kita 8 Nishi 18-35-100, Chuo-ku, Sapporo 060-0008, Japan
TEL: +81-11-624-5860 FAX: +81-11-351-1822
MAIL: info_itnl@goryochemical.com URL: https://goryochemical.com/en/

EAREE Bldg. 5F Kita 8 Nishi 18-35-100, Chuo-ku, Sapporo 060-0008, Japan
TEL: +81-11-624-5860 FAX: +81-11-351-1822
MAIL: info_itnl@goryochemical.com URL: https://goryochemical.com/en/For research use only. Not for diagnostic use.

FL0080GR 2019/02

goryochemical.com/en/30μm

SaraFluor TM Series, fluorophores for labeling from GORYO Chemical

SaraFluorTM for general labeling protein and other molecules

SaraFluor TM Series, fluorophores for labeling from GORYO Chemical

SaraFluorTM B※ for super-resolution live-cell imaging

Wide wavelength range (Green: 488 〜 NIR: 720)

Developed exclusively for SMLM, SaraFluor B Series is a fluorescent probe that exhibits spontaneous blinking under physiologicalconditions. The lineup includes SaraFluor 650B (SF650B, HMSiR) which emits deep red light with red laser excitation, and SaraFluor
488B (SF488B, HEtetTFER) which emits green light with blue laser excitation. Target molecules can be labeled by HaloTag, NHS ormaleimide.

Excellent photostability
Various reactive groups (-NHS, -maleimide, -NH2, -COOH)
SaraFluor series is a product line of bright fluorophores, which are suitable for labeling proteins (e.g., antibodies) and other macromolecules.
These products are also used for labeling, marking or to evaluate physical properties. Among them, SaraFluor 650, 700, and 720, derivativesof silicone rhodamine, are uniquely bright and photostable fluorophores in far-red to near-infrared wavelength range.
N-hydroxysuccinimide (NHS) esters quickly form a covalent bond with primary amine just by mixing. NHS esters are widely used for labelingantibodies and macromolecules. On the other hand, maleimides which forms a covalent bond with thiols (R-SH) are used to specifically label
cysteine residues. The fluorophores with amino groups (-NH2) and carboxyl groups (-COOH) are used for labeling using crosslinkers, usedas a material for chemical synthesis.

Feature 1

Available for super-resolution imaging under the physiological condition
Fig.1 Super-resolution imaging of A549 cell
Conventional fluorescence and SMLM images of fixed A549
c ell st ained with anti - α - tubulin antibodies (D M1A 1/40 0 0dilution) and SaraFluor 488B labeled secondar y antibody.
The total internal reflection fluorescence microscopy images
were taken using NIKON Ti, NIKON Apo TIRF100x (NA 1.49)
a n d I m a g EM (H a m a m at su Ph oto ni c s) w i t h 4 8 8 nm las e rexcitation. The SMLM image was processed using ImageJand ThunderSTORM.

Feature 1 Wide wavelength range (Green: 488 〜 NIR: 720)

Feature 2

Fig.1 Spectra of SaraFluor series(dot lines: absorption spectra, solid lines: emission spectra)

Feature 2 Excellent photostability

Blinking without high-power laser irradiation

Fig.2 Spontaneous blinking of SaraFluor B in PBS (pH 7.4)
Measured intensity change in a region corresponding to one
molecule from a fluorescence image obtained by total internalreflection fluorescence microscopy with 647 nm laser excitation(SaraFluor 650B, HEtetTFER).
It is impossible to compare two vertical axes because different
optical measurement systems were used.

SaraFluor 650

SaraFluorTM T※ with HaloTag technology

T: turn-on

SaraFluor 650T (SF650) is a fluorophore based on a silicone rhodamine. It is conjugated with HaloTag ligand which immediatelyforms a covalent bond with HaloTag when mixed. Under physiologic conditions, this fluorophore alone shows weak fluorescence. It
strongly fluoresces upon binding with HaloTag proteins. This feature enables high-contrast imaging without washing out the unboundfluorophore. It is suitable for fluorescence live cell imaging, single molecule imaging, and super-resolution imaging.

Product AFeature

Turn-on probe enables imaging with high contrast
HaloTag
LigandHaloTagFig.2 A comparison of photobleaching property of SaraFluor 650
Fixed cells were stained with secondary antibodies labeled with SaraFluor 650 or another corresponding fluorophore, and wereobserved by fluorescence microscopy. Photobleaching was observed under the same imaging conditions including light intensity,
fluorescent filters, exposure, and camera gain.

NHS ester (N-hydroxysuccinimide ester)

SaraFluor 650T (ON)Fig.1 Spectra of HaloTag SaraFluor 650T Ligand upon combined andnot combined with HaloTag protein
Absorbance and fluorescence spectra were measured in the presence(with)and absence(w/o) of HaloTag protein, respectively. The addition of HaloTagprotein increased absorbance and fluorescence intensity(Turn-on probe).
®

Primary Amineon Protein

Imaging application

maleimide

pH 7-9++Stable Conjugate(amide bond)

pH 6.5-7.5+NHSMaleimideReagent

SaraFluor 650T (OFF)HaloTagFeature 3 Various reactive groups (-NHS, -maleimide, -NH2, -COOH)NHS EsterReagent

B: blinking

Sulfhydrylon Protein

Stable Conjugate(thioeher bond)Fig.3 SaraFluor Series offers maleimide, amino (-NH2) and carboxyl (-COOH) in addition to NHS- form (N-hydroxysuccinimide ester)
NHS esters quickly form a covalent bond with primary amine just by mixing. NHS esters are widely used for labeling antibodies andmacromolecules. On the other hand, maleimides which forms a covalent bond with thiols (R-SH) are used to specifically label cysteine
residues. The fluorophores with amino groups (-NH2) and carboxyl groups (-COOH) are used for labeling using crosslinkers, used asa material for chemical synthesis.

Fig.2 Intracellular microtubule network
visualized with HaloTag® SaraFluor 650T Ligand
Microtubules imaged using HaloTag ® SaraFluor
650T Ligand. Left, confocal microscopy image.
Right, STED microscopy image. Data was kindly
provided by Prof. Dr. Yasushi Okada (RIKEN QBiC;Grad. School of Sci, Univ. Tokyo)