Academic achievements
Assistant Professor

Taichi Takasuka PhD.

Hokkaido University
Research Faculty of Agriculture
Kita9 Nishi9 Kita-ku Sapporo
Tel: +81 011-706-4962
Email: takasuka@cen.agr.hokudai.ac.jp
論 文 (http://www.researchgate.net/profile/Taichi_Takasuka)
査読付き論文1. Book, A.J., Lewin, G.R., McDonald B.R., Takasuka, T.E., Doering, D.T., Adams, A.S., Blodgett, J.A.V.,
Clardy, J., Raffa, K.F., Fox , B.G., and Currie, C.R. (2014) “Cellulolytic Streptomyces strains associatedwith herbivorous insects share a phylogenetically-linked capacity for the degradation of lignocellulose”
Appl. Environ. Microbiol., in press
2. Book A.J.*, Yannamalli, R.M.*, Takasuka, T.E.*, Currie, C.R., Phillips, G.N.Jr., and Fox, B.G. “Evolutionof substrate specificity in bacterial AA10 lytic polysaccharide monooxygenases” Biotechnol Biofuels., inpress
3. Deng, K., Takasuka, T.E., Heins, R., Cheng, X., Bergeman, L.F., Shi, J., Achenbrener, R., Deutsch, S.,Singh, S., Sale, K.L., Simmons, B.A., Adams, P.D., Singh, A.K., Fox, B.G., and Northen, T.R. (2014)“Rapid Kinetic Characterization of Glycosyl Hydrolases Based on Oxime Derivatization and NanostructureInitiator Mass Spectrometry (NIMS)” ACS Chem. Biol., in press
4. Takasuka, T.E., Acheson, J.F, Bianchetti, C.M., Prom, B.M., Bergeman, L.F, Book, A.J, Currie, C.R., andFox, B.G. (2014) “Atomic resolution structure and biochemical properties of a proteolytically processed βmannanase from cellulolytic Streptomyces sp. SirexAA-E” PLoS One 9, e94166
5. Takasuka, T.E.†, Hsieh, Y.J., and Stein, A. (2014) “Miniaturized sequencing gel analysis for DNA
footprinting” Appl. Biochem. Biotechnol., 172, 1-8
6. Takasuka, T.E.*, Bianchetti, C.M.*, Tobimatsu, Y., Bergeman, L.F, Ralph, J., and Fox, B.G. (2013)“Structure and function of abundantly secreted chitosanase SACTE_5457 from Streptomyces sp. SirexAAE.” Proteins: Str. Func. And Bioinfo., DOI: 10.1002/prot.24491
7. Bianchetti, C.M., Harmann, C.H., Takasuka, T.E., Hura, G.L., Dyer,K, and Fox, B.G. (2013) “Fusion ofDioxygenase and Carbohydrate-Binding Module in a Novel Secreted Caffeoyl-CoA Dioxygenase from
Cellulolytic Streptomyces sp. SirexAA-E” J. Biol. Chem., 288 18574-18587
8. Chandrasekaran, A. Deng, K., Koh, C.Y., Takasuka, T., Bergeman, L.F., Fox, B.G., Adams, P, and Singh,A.K. (2013) “A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan
microspheres.” ChemComm., 49, 5441-5443
9. Takasuka, T.E.*, Book, A.J.*, Lewin, G.R., Currie, C.R., and Fox, B.G. (2013) “Aerobic deconstruction ofcellulosic biomass by an insect-associated Streptomyces.” Nat. Sci. Rep., 3, 1030
10. Riederer, A., Takasuka, T.E., Makino, S., Stevenson, D.M., Bukhman, Y., Elsen, N.L., and Fox, B.G.
(2011) “Global gene expression patterns in Clostridium thermocellum from microarray analysis of chemostat
culture on cellulose or cellobiose.” Appl. Environ. Microbiol., 77, 1243-1253

TAKASUKA, Academic achievements Mar 20151

11. Miller, A., Chen, J., Takasuka, T.E., Kaufman, P.D., Jacobi, J.L., Irudayaraj, J.M.K., and Kirchmaier, A.L.
(2010) “Proliferating cell nuclear antigen (PCNA) is required for cell- cycle regulated silent chromatin on
replicated and nonreplicated genes.” J Biol. Chem., 285, 35142-35154
12. Takasuka, T.E., and Stein, A. (2010) “Direct measurements of the nucleosome-forming preferences ofperiodic DNA motifs challenge established models.” Nucl. Acids. Res., 38, 5672-5680
13. Stein, A., Takasuka, T.E., and Collings, C.K. (2010) “Are nucleosome positions in vivo primarily
determined by histone-DNA sequence preferences?” Nucl. Acids. Res., 38, 709-71914. Takasuka, T.E., Cioffi, A., and Stein, A. (2008) “Sequence information encoded in DNA that may
influence long-range chromatin structure correlates with human chromosome functions.” PLoS One, 3 e2643†Correspondence, * Equally contributed,

著書
1. Takasuka, T.E., Walker, J.A., Bergeman, L.F., Vander Meulen, K.A., Makino, S.I., Elsen, N.L., Fox, B.G.
(2014) “Cell-free translation of biofuels enzymes.” Methods. Mol. Biol., DOI: 10.1007/978-1-62703-7822_5
学 会 発 表 (2010-present)
口頭発表
1. DOE – Great Lakes Bioenergy Research Center annual meeting, South Bend, IN (2014)
Title: Genome-enabled Biochemical and Structural Characterization of GH5 Enzymes
2. Invited talk at University of California, Santa Cruz, Santa Cruz, CA (2014)
Title: Genome-enabled Discovery of Biomass-degrading Enzymes for Bioenergy Production
Host: Prof. Harry F. Noller
3. DOE – Great Lakes Bioenergy Research Center annual meeting, South Bend, IN (2013)
Title: Biochemical and Structural Characterization of an Engineered Tri-functional GH5 Cellulase
4. University of Wisconsin – Madison, Biochemistry Seminar, Madison, WI (2013)
Title: Atomic resolution structure and biochemical properties of a proteolytically processed beta-mannanasefrom cellulolytic Streptomyces sp. SirexAA-E
5. The American Institute of Chemical Engineers Annual Meeting, Pittsburgh, PA (2013)
Title: Discovery and functional determinations of biomass-degrading enzymes by robotic cell-freetranslation.
6. DOE – Great Lakes Bioenergy Research Center annual meeting, Chicago, IL (2012)
Title: Discovery and functional determination of biomass-degrading enzymes.
7. The American Institute of Chemical Engineers Annual Meeting, Minneapolis, MN (2012)
Title: Discovery and characterization of cellulolytic enzymes from the wood wasp symbiont Streptomyces
sp. ActE.
8. Invited talk at Ehime University, CSTRC VBL seminar, Ehime, Japan (2011)
Title: Coherent genomic and proteomic responses for naturally optimized biomass degrading system of theinsect symbiont Streptomyces sp. ActE.

TAKASUKA, Academic achievements Mar 20152

Host: Prof. Tatsuya Sawasaki
9. DOE – Great Lakes Bioenergy Research Center annual meeting, South Bend, IN (2011)
Title: Discovery and characterization of cellulolytic enzymes from the wood wasp symbiont Streptomyces
sp. ActE.
ポスター発表1. The 37th Annual Meeting of the Molecular Biology Society of Japan, Yokohama, Japan (2014)
Title: Structure and functional characterization of laminarinase from Streptomyces sp. SirexAA-E
2. DOE – Great Lakes Bioenergy Research Center annual meeting, South Bend, IN (2014)
Title: Structural and functional determination of novel multifunctional enzymes by the high-throughput genesynthesis, protein translation and the state-of-art functional platform.
3. DOE – Great Lakes Bioenergy Research Center annual meeting, South Bend, IN (2014)
Title: Structural and functional study of substrate bound mode of bacterial GH55 enzyme.
4. 2014 Genomic Science Contractors – Grantees Meeting, Bathesda, MD (2014)
Title: Contribution of multifunctional enzymes to biomass hydrolysis.
5. The 36th Annual Meeting of the Molecular Biology Society of Japan, Kobe, Japan (2013)
Title: High throughput functional screening, Biochemical and Structural Characterization of Multifunctional Cellulase.
6. DOE – Great Lakes Bioenergy Research Center annual meeting, South Bend, IN (2013)
Title: Structural and functional analysis for the multi-functional cellulases
7. The Annual Midwest Enzyme Meeting, Chicago, IL (2012)
Title: High-throughput protein production and the multi-enzyme assay platform for biomass-degradingenzymes.
8. 2012 Genomic Science Contractors – Grantees Meeting, Bathesda, MD (2012)
Title: Systems – level discovery and characterization of cellulolytic enzymes from the wood wasp symbiont
Streptomyces.
9. DOE – Great Lakes Bioenergy Research Center annual meeting, South Bend, IN (2010)
Title: Combinatorial discovery of enzymes and proteins for biomass deconstruction.
特許• Takasuka T.E., Bianchetti, C.M., and Fox, B.G. (2012) Multifunctional cellulase/hemicellulase.
P120371US02.
•Fox, B.G., Takasuka T.E., Book, A.J., and Currie, C.R. (2011) Methods and compositions for improved
lignocellulosic materials hydrolysis. P110314US01

教育Lecturer Department of Biochemistry, University of Wisconsin – Madison
Biochem909: Biochemistry seminar (one semesters)
Teaching instructor Department of Biological Sciences, Purdue University
BIOL221: Microbiology (three semesters of laboratory and lecture)

TAKASUKA, Academic achievements Mar 20152013 – present
2006 – 20093

BIOL542: Chromatin lab (two semester of laboratory and lecture)
BIOL573: Molecular biology (two semesters of lecture)
Teaching assistant Department of Biochemistry, Purdue UniversityBCHM309: Introductory biochemistry (three semesters of laboratory and lecture)
RESEARCH STUDENT MENTORING, University of Wisconsin – Madison
Ms. Hannah Udell* (B.S., 2014)
Mr. Ben Prom* (B.S., 2013)
Mr. Connor Harmann* (B.S., 2014)
Ms. Meredith Hinkes (B.S., 2014)
Mr. Johnnie A. Walker*† (Ph.D. Dissertator in Biophysics Degree Program)
Mr. Justin F. Acheson* (Ph.D. Dissertator in Department of Biochemistry)
Mr. Ryan Aschenbrener* medical student, University of Cincinnati (B.S., 2013)
Ms. Erin Bulleit medical student, University of Rochester (B.S., 2010)* Students receiving coauthorship on publications, †Advised during thesis
学会会員• American Chemical Society•

The American Society for Biochemistry and Molecular Biology

2004 – 2005

2013 – present
2012 – 20132011 – present2012
2010 – present2010 – present2010 – 20122010 – 20112013 – present
2008 – 2010

アウトリーチ活動
I have participated in K-16 (students from kindergarten up to college senior) educational outreachprograms as part of the US Department of Energy – Great Lakes Bioenergy Research Center by giving apresentation about biofuels, and also mentored high school students during the summer. Additionally, Ihave participated in outreach activities to share my experience and love of science to retirees in the
Madison community (Wisconsin, USA).

TAKASUKA, Academic achievements Mar 20154