Incucyte®:
Reagents, Consumables
and SoftwareIncucyte Reagents, Consumables
and Software®

Incucyte® Reagents At-a-Glance
Application

Reagent

Consumable

SoftwareModuleCompatible Instrument
SX5

Sartorius offers a range of reagents, consumables andsoftware specifically designed around your applicationneeds to enable long-term, kinetic live-cell imaging
and analysis. Obtain specific and robust measurementsof cell health, movement and function with optimized,
turnkey solutions allowing you to connect phenotype andfunction with pathological processes. Getting biologically
relevant information has never been easier!

Find Your Solution to Meet Your Application Needs:--

Cell Health
Proliferation:
Label-free, Confluence
Label-free, Cell Counting
Fluorescent Labeling, Cell Counting

Viability
Cell Cycle
Apoptosis
Cytotoxicity

Cell Health

Cell Function

Proliferation

Immune Cell Killing

Viability

Antibody Internalization

Cell Cycle

Live-Cell Immunocytochemistry

Immune Cell Killing

Apoptosis

Phagocytosis

Antibody Internalization

Cytotoxicity

NETosisLive-Cell Immunocytochemistry

Mitochondrial Membrane Potential

Kinase Activity

ATP Metabolism

Neuronal Activity

Mitochondrial Membrane Potential
ATP Metabolism
Advanced Label-Free Classification Analysis
Cell Function

Phagocytosis
NETosisKinase Activity
Neuronal Activity--

Cell Movement and Morphology

Assays for 3D Models

Chemotaxis Migration and Invasion

Spheroid Growth

Scratch Wound Migration and Invasion

Spheroid Invasion

Immune Cell Activation and Proliferation

Spheroid Immune Cell Killing

Neurite Outgrowth

Neurite Outgrowth

Organoid Culture QC

Advanced Label-Free Classification Analysis

Organoid Assay

Assays for 3D Models

Key Advantages
Derive meaningful data with sensitive, non-perturbing
reagents for long term, live-cell studies.
Unlock your productivity with lab-tested protocols andpurpose-built, intuitive software.

Find out more: www.sartorius.com/incucyte--

Cell Movement and Morphology
Chemotaxis Migration and Invasion
Scratch Wound Migration and Invasion
Immune Cell Activation and Proliferation

Spheroid Growth (Single and Multi-Spheroid)
Spheroid Invasion (Single Spheroid)

Generate data rich information within every sample withfluorescent reagent combinations.
Support visualization and automation of cell movement
studies with Incucyte® consumables.

Spheroid Immune Cell Killing
Organoid Culture QC
Organoid Assay
Required  

Optional  

Not required  Compatible

S3

SX1

Application Spotlight: Incucyte® Cell Cycle Assay

Proliferation, Cell Counting,Viability and Cell Cycle

Minimize experimental artifacts using non-perturbing,
live-cell reagents—preservative free, sterile, orconcentrated formulations to protect cell healthLabel-Free Classification of Cells in Mitosis
20
15
10
5
0

Application Spotlight:
Incucyte® Live-Cell Proliferation Assays
Perform long-term, kinetic measurements of proliferation,with or without labels. Incucyte® Nuclight Reagents
homogenously label a variety of cell types for non-

24

36
48
Time (h)60

Product
Softwareperturbing, continuous analysis of the same population ofcells. The Incucyte® Cell-by-Cell Analysis Software Module
enables label-free cell identification and counting.

*Nuclear Lentivirus
Labeling Reagents

Description

HT-1080 Nuclight Cell Tri-Culture
Orange Cells
34%

Green Cells
36%

500
0

0

24

48
Time (h)72

Cell Count

0

24

36

Time (h)48

60

New! Incucyte® Advanced Label-FreeClassification Analysis Software Module

1 module

BA-04867

SX5, S3, SX1

Cell-permeable DNA stains that specifically label nuclei in cells and are ideally suited for mix-and-read, live-cell
quantification of cell proliferation and viability.
Incucyte® Nuclight Rapid Red Dye

One vial: 50 µL

4717

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Nuclight Rapid NIR Dye

One vial: 50 µL

4804

SX5

Lentivirus reagents provide homogenous expression of a nuclear-restricted fluorescent protein without altering cellfunction for live-cell quantification of cell proliferation and viability.

HT-1080 fibrosarcoma cells stably expressing Nuclight Green, Orange, or
NIR were monitored for 72 hours. Representative images taken at 48 hours,with and without the label-free Cell-by-Cell Analysis mask, automatically
identify the entire population of cells and quantify percentages of green,orange or NIR expressing cells.

0.2 mL4624

SX5, S3, SX1

0.6 mL

4475

SX5, S3, SX1

0.2 mL

4625

0.6 mL

4476

SX5 (configured with Green/Red
Optical Module), S3, SX1

0.2 mL

4626

SX5, S3, SX1

0.6 mL

4477

SX5, S3, SX1

0.2 mL4627

0.6 mL

4478

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Nuclight Orange Lentivirus (puro)

0.2 mL

4771

SX5

Incucyte Nuclight NIR Lentivirus (puro)

0.2 mL

4805

SX5

®

NIR Cells
30%

Instrument Compatibility

SX5, S3, SX1

Incucyte Nuclight Red Lentivirus (bleo)

Cell Cycle
Lentivirus ReagentsCat. No.

9600-0031

®

2000

1000

20

1 module

Incucyte® Nuclight Green Lentivirus (bleo)

Green Cell Count
Orange Cell CountNIR Cell Count

40

Incucyte® Cell-by-Cell Analysis
Software Module

Incucyte® Nuclight Red Lentivirus (puro)

1500

60

Perform label-free cell counts and subsequent cell-by-cell classification based on shape, size or fluorescence intensity
to quantify dynamic changes in cell subsets within heterogeneous living cell cultures with Cell-by-Cell Analysis Software
Module. Perform counts and track changes in adherent cell morphology via label-free image segmentation and multivariate
analysis of cell shape. Classifier is trained using control wells. Advanced Label-Free Classification Software Module an addon and requires Incucyte® Cell-by-Cell Analysis Software Module (Cat. No. 9600-0031).

Incucyte® Nuclight Green Lentivirus (puro)

Total Cell Count

80

Ordering Information

Nuclear DyeLabeling Reagents

2500

Fluorescence Classification of Cell Cycle Phase
Cell Cycle Population (%)---

Quantify cell proliferation kinetically using label or labelfree strategies in adherent or non-adherent cell cultures
Investigate a range of cell models (mono-, co- or
tri-culture) to answer relevant scientific questions
Maximize each sample with multiplexed readouts ofproliferation, cell cycle or cell health that are easily
validated by eye

Mitotic (%)

Incucyte® live-cell labeling reagents and purposebuilt software enable long-term (> 48 h) analysis of cellproliferation, viability and cell cycle state.

Continuously quantify treatment effects on cell cycle progression of the same population of cells
over multiple cell divisions using Incucyte® Cell Cycle Lentivirus Reagents. Gain deeper insight bymultiplexing with Incucyte® Annexin V NIR Dye on the Incucyte® SX5.

Fluorescent ubiquitination-based cell cycle indicators (FUCCI), providing a homogeneous expression of paired
fluorescent proteins to distinguish between cells in the interphase or the mitotic phase.
Incucyte® Cell Cycle Green/Red Lentivirus(puro)

0.2 mL

4779

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Cell Cycle Green/Orange Lentivirus(puro)

0.2 mL

4809

SX5* Pre-labeled Nuclight cell lines are also available for purchase. Please visit www.sartorius.com/shop for more information.

Ordering Information

Apoptosis, Cytotoxicity, Mitochondrial
Membrane Potential and ATP Metabolism
Incucyte® non-perturbing cell health reagents allow for
kinetic readouts over multiple days for the evaluation oftime-dependent and cell-specific responses to treatments.

-

Preserve cell health using non-perturbing, live-cellreagents to track true, artifact-free biological responses-

Product
Software

Reduce loss of precious or compromised cells with mixand-read or novel genetically encoded reagents andoptimized protocols
Perform multi-parametric analysis using cell healthreagent combinations within a single well to generate
data rich information
Validate results and confirm changes in cell morphology
with high density (HD) phase images at every time point

Application Spotlight:
Incucyte® Apoptosis Assay
Annexin V Dyes to measure exposed phosphatidylserine
in apoptotic cells. Enhance your insight with multiplexed
measurements of nuclear counts of cell death.

600

Control

NIR Nuclear Count

500
0.001 µM

4000.004 µM

300
0.01 µM

2000.04 µM
0.11 µM
0.33 µM
1 µM

100
0

0

12

24
Time (h)36

48

9600-0031

SX5, S3, SX1

Incucyte® Advanced Label-FreeClassification Analysis Software Module

1 module

BA-04867

SX5, S3, SX1

Inert, non-fluorescent (DEVD) substrates that freely cross the cell membrane where they can be cleaved by activatedcaspase-3/7 to release a DNA-binding fluorescent label.

Cytotoxicity
Reagents

0.04 µM

2000.01 µM
0.004 µM
0.001 µM
Control01224Time (h)36

48

HT-1080 fibrosarcoma cells stably expressing Nuclight NIR Lentivirus (pseudo-colored blue) were treated with decreasing concentrations ofcamptothecin in the presence of Incucyte® Caspase-3/7 Green Dye (pseudo-colored green). Representative images validate kinetic data of bothcell viability and apoptotic death.

SX5, S3, SX1

Incucyte® Caspase-3/7 Red Dye

One vial: 20 µL(100–200 tests)

4704

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Caspase-3/7 Dyefor Metabolism

One vial: 20 µL(100–200 tests)

4776

SX5 (configured with SX5Metabolism Optical Module)

Incucyte® Annexin V Green Dye

One vial: 100–200 tests

4642

SX5, S3, SX1

Incucyte® Annexin V Red Dye

One vial: 100–200 tests

4641

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Annexin V Orange Dye

One vial: 100–200 tests

4759

SX5

Incucyte® Annexin V NIR Dye

One vial: 100–200 tests

4768

SX5

Incucyte® Cytotox Green Dye

Five vials: 5 µL
(100 tests each)4633

SX5, S3, SX1

Incucyte® Cytotox Red Dye

Five vials: 5 µL
(100 tests each)4632

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Cytotox NIR Dye

One vial: 100 uL(500–100 tests)

4846

SX5

--

New! Incucyte® MMP Orange Reagent Kit:

­

40

4440

Fluorescent dye that diffuses across the intermembrane space and accumulates in proportion to the MMP. Shifts
in fluorescent intensity denote mitochondrial membrane potential state. Requires Incucyte® Cell-by-Cell Analysis
Software Module for analysis.

80

60

One vial: 20 µL(100–200 tests)

Highly sensitive cyanine-based dyes that do not enter living cells. When the cell membrane is compromised, it enters thecell, binds to DNA, and becomes fluorescent. Dye does not enter cells with intact cell membranes.

­

0.11 µM
0.33 µM
1 µM

Incucyte® Caspase-3/7 Green Dye

Membrane impermeable, highly-selective phosphatidylserine (PS) cyanine fluorescent dyes label PS exposure on theextracellular surface of cells undergoing apoptosis.

1 µM Camptothecin

Green Caspase-Positive Cells (%)

Control

Instrument Compatibility

1 module

Caspase Activity
Reagents

New!
Mitochondrial
Membrane Potential(MMP)

Cat. No.

Incucyte® Cell-by-Cell Analysis
Software Module

Apoptosis Plasma
Membrane Integrity
Reagents

Evaluate cell death with two unique assays for apoptotic
pathway analysis using specifically formulated reagents.
Utilize Incucyte® Caspase-3/7 Dyes to allow direct
detection of caspase-3/7 activation or Incucyte®

Description

Perform label-free cell counts and subsequent cell-by-cell classification based on shape, size or fluorescence intensity
to quantify dynamic changes in cell subsets within heterogeneous living cell cultures with Cell-by-Cell Analysis Software
Module. Perform counts and track changes in adherent cell morphology via label-free image segmentation and multivariate
analysis of cell shape. Classifier is trained using control wells. Advanced Label-Free Classification Software Module an addon and requires Incucyte® Cell-by-Cell Analysis Software Module (Cat. No. 9600-0031).

­

One kit

MMP Orange Dye

One vial: 30 μL(200 tests)

FCCP

One vial: 10 μL(16 tests)

Oligomycin A

One vial: 10 μL(16 tests)

4775

SX5

New!
ATP Metabolism

Direct, kinetic measurement of ATP to analyze changes of cancer cell metabolism in advanced cell models.

SoftwareEnables analysis of ATP dynamics by capturing fluorescent images while qualitatively monitoring associated changes incell morphology in each well of a 96- or 384-well plate.
New! Incucyte® ATP Analysis Software
ModuleMetabolism
Reagent

1 module

9600-0033

SX5 (configured with SX5Metabolism Optical Module)

Genetically-encoded fluorescent ATP indicator for direct measurements of cytosolic ATP in living cells.
New! Incucyte® CytoATP Lentivirus(puro)One vial: 0.2 mL

4772

SX5 (configured with SX5Metabolism Optical Module)

Find out more: www.sartorius.com/incucyte

Ordering Information

Immune Cell Activation,
Killing and NETosis
Incucyte® Immune Cell Assays are an integrated solution
for real-time visualization and automated analysis of arange of immune cell functions from T cell activation andkilling to programmed neutrophil cell death.

Product--

SoftwareDerive meaningful data with sensitive, non-perturbing
reagents and HD phase images
Make multiplexed measurements of death, viability andproliferation in 2D or 3D in vitro assay models
Visualize and quantify dynamic cell interactions overtime
in complex co-cultures*Nuclear Lentivirus
Labeling Reagents

Description

1 module

9600-0031

SX5, S3, SX1

New! Incucyte® Advanced Label-FreeClassification Analysis Software Module

1 module

BA-04867

SX5, S3, SX1

Lentivirus reagents provide homogenous expression of a nuclear-restricted fluorescent protein without alteringcell function for live-cell quantification of cell proliferation and viability.
Incucyte® Nuclight Green
Lentivirus (puro)

0.2 mL4624

SX5, S3, SX1

0.6 mL

4475

SX5, S3, SX1

Incucyte Nuclight Red
Lentivirus (puro)

0.2 mL

4625

0.6 mL

4476

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Nuclight Green
Lentivirus (bleo)

0.2 mL

4626

SX5, S3, SX1

0.6 mL

4477

SX5, S3, SX1

Incucyte Nuclight Red
Lentivirus (bleo)

0.2 mL4627

0.6 mL

4478

SX5 (configured with Green/Red
Optical Module), S3, SX1

NIR Total Area (x 10 4)

Capture, visualize and automatically quantify dynamic
biological changes and cellular interactions of immune
cell-mediated killing of tumor cells. With Incucyte® cell
health and proliferation reagents you can make multiplexed
measurements of tumor cell death (Annexin V NIR Dye),tumor cell proliferation/viability (Incucyte® Nuclight Orange
Lentivirus) and immune cell health (Incucyte® Cytolight
Rapid Green Dye) in the same population of cells over time.

®

Target Cell Death (Annexin V NIR)
2.5
Activated

2.0

Cytoplasmic DyeLabeling Reagents

1.5
1.0
Non-activated

0.5
0

0

12

24
36
Time (h)48

60
Caspase Activity
Reagents

Orange Object Count/Well

Target Cell Proliferation/Viability(Nuclight Orange)

Green Object Count/Well

4771

SX5

Incucyte® Nuclight NIR Lentivirus (puro)

4805

SX5

300

Incucyte® Cytolight Rapid Green Dye

One vial: 15 μg

4705

SX5, S3, SX1

Incucyte Cytolight Rapid Red Dye

Five vials: 50 μg

4706

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Cytolight Rapid Orange Dye

One vial: 1 mg

4839

SX5

Inert, non-fluorescent (DEVD) substrates that freely cross the cell membrane where they can be cleaved by activatedcaspase-3/7 to release a DNA-binding fluorescent label. Recommended for quantifying apoptosis in adherent target cells.
Incucyte® Caspase-3/7 Green Dye

One vial: 20 µL(100–200 tests)

4440

SX5, S3, SX1

Incucyte® Caspase-3/7 Red Dye

One vial: 20 µL(100–200 tests)

4704

SX5 (configured with Green/Red
Optical Module), S3, SX1

No PBMC control
Apoptosis Plasma
Membrane Integrity
Reagents200
Non-activated

100
0

0

12

24

36
Time (h)48

60
Cytotoxicity
Reagents
Activated PBMC

300
200
100
0

Non-activated
PBMC
0

12

24
36
Time (h)48

60

Membrane impermeable, highly-selective phosphatidylserine (PS) cyanine fluorescent dyes label PS exposed on theextracellular surface of cells undergoing apoptosis. Recommended for quantifying apoptosis in non-adherent target cells.
Incucyte® Annexin V Green Dye

One vial: 100–200 tests

4642

SX5, S3, SX1

Incucyte Annexin V Red Dye

One vial: 100–200 tests

4641

SX5 (configured with Green/Red
Optical Module), S3, SX1®

Activated

0.2 mL

Live-cell cytoplasmic labeling dyes that freely pass through cell membranes and into cells, where they are transformed
into a cell membrane-impermeant form, providing spatial context for cell-to-cell interactions. Use to label either target
or effector cells.

400

Effector Cell Proliferation(Cytolight Rapid Green)

MDA-MB-231 adenocarcinoma cells transduced with Incucyte®
Nuclight Orange co-cultured with Incucyte® Cytolight Rapid Greenlabeled activated or non-activated PBMCs in the presence ofIncucyte® Annexin V NIR. Quantification of NIR (pseudo-colored blue)
fluorescence area indicates target cell death and object count of orange(pseudo-colored red) fluorescence, target cell proliferation/viability(pseudo-colored green). Effector cell proliferation was quantified
based on green object count over the course of the experiment.

Incucyte® Nuclight Orange Lentivirus (puro) 0.2 mL®

No PBMC control

Instrument Compatibility

Incucyte® Cell-by-Cell Analysis
Software Module®

Application Spotlight:
Incucyte® Immune Cell Killing

Cat. No.

Perform label-free cell counts and subsequent cell-by-cell classification to evaluate changes in proliferation, morphology
and cell surface protein expression for evaluation of immune cell activation and proliferation in mono-cultures or track
subsets of cells in complex immune-cell killing models with Cell-by-Cell Analysis Software Module. Perform counts andtrack changes in adherent cell morphology via label-free image segmentation and multivariate analysis of cell shape.
Classifier is trained using control wells. Advanced Label-Free Classification Software Module an add-on and requires
Incucyte® Cell-by-Cell Analysis Software Module (Cat. No. 9600-0031).

Incucyte® Annexin V Orange Dye

One vial: 100–200 tests

4759

SX5

Incucyte® Annexin V NIR Dye

One vial: 100–200 tests

4768

SX5

Highly sensitive cyanine-based dyes enter the cell when cell membrane is compromised, and become fluorescent uponbinding to DNA. Dye does not enter cells with intact cell membranes. Allows for rapid visualization and quantificationof NETosis as extracellular DNA is released and undergoes fluorescence enhancement.
Incucyte® Cytotox Green Dye

Five vials: 5 µL
(100 tests each)4633

SX5, S3, SX1

Incucyte® Cytotox Red Dye

Five vials: 5 µL
(100 tests each)4632

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Cytotox NIR Dye

One vial: 100 uL(500–100 tests)

4846

SX5

* Pre-labeled Nuclight cell lines are also available for purchase.
Please visit www.sartorius.com/shop for more information.

Find out more: www.sartorius.com/incucyte

Application Spotlight:
Antibody Internalization-

Increase productivity with rapid single-step labelingpaired with mix-and-read protocols for efficient testingof antibody panels
Associate changes in surface protein expression
or antibody internalization with cell function andmorphology over time
Combine sensitive, kinetic fluorescent measurementof protein dynamics with images and movies for visualconfirmation of biology in every well

Application Spotlight:
Monitoring Dynamic Cell Surface Protein Expression

HD phase and orange fluorescence images (10X)
show HER-2 positive BT-474 cells treated with
Incucyte® Fabfluor-pH Orange labeled Herceptin
display orange (pseudo-colored red), cytosolic
fluorescence while cells treated with an isotype
control display no cellular fluorescence. Time-course
data shows a rapid increase in orange object area overtime in cells treated with labeled Herceptin, but notwith IgG1 isotype control.

3 x 1033
40 x 10405

Fabfluor-pH
Antibody Labeling
Reagents

3.1 ng/mL
1.6 ng/mL
No IFNγ

2

0

24
48
Time (h)72

Green Area (µm2/well)

Green Area (µm2/well)12.5 ng/mL
6.3 ng/mL

0

Day 2

6

4

Fabfluor Live-Cell
Immunocytochemistry
Labeling Reagents

4

2

0

1
10
100
log [IFNγ] (ng/mL)

IgG-Fabfluor

0
0

12

24
24

Time (h)

36
36

48
48

2 x 1032

1 x 1031

IgG-FabFluor
media

00
-8
-7
-6
-5
-8
-7
-6
-5
[Herceptin] (g/mL)
log Log[Herceptin](g/mL)

Ordering Information

Incucyte® Fabfluor-488 was conjugated toanti-PD-L1 Ab (BioLegend) and added to
Nuclight Red MDA-MB-231 breast cancer
cells in the absence and presence of IFNγ(+ Incucyte® Opti-Green backgroundsuppressor). Quantification of the green
fluorescent area shows that IFNγ induces
a time- and concentration-dependent
increase in PD-L1 expression.

Description

Cat. No.

Instrument Compatibility

Perform label-free cell counts and quantify dynamic changes in cell subsets within heterogeneous living cell cultures.
Incucyte® Cell-by-Cell Analysis
Software Module

50 ng/mL
25 ng/mL

Herceptin-Fabfluor

20 x 10205

00

Software6

4 x 1034

60 xx 1060
1055

Product

Day 1

Herceptin-FabFluor

Media

Quantify cell surface protein expression and distribution in live cells to study long-term
protein dynamics and their relationship to function and morphology using Incucyte®
Fabfluor-488 or Fabfluor-594 Antibody Labeling Reagents.

Day 0

IgG—Fabfluor-pH Orange

Herceptin—Fabfluor-pH Orange
Orange Object Area(µm2/well)

AUC (0—48 h)

Incucyte® antibody labeling reagents are novel fluorescently labeled Fabs that can be mixed with Fc-containing
antibodies and applied directly to living cells for long-termmonitoring of spatial and temporal protein dynamics.

Efficiently evaluate the full time course ofantibody internalization for real-time analysis
of internalization rates under physiologicalconditions using Incucyte® Fabfluor-pH
Antibody Labeling Reagents.

Orange Object (µm2/well)

Antibody Internalization and
Immunocytochemistry

1 module

9600-0031

SX5, S3, SX1

Novel pH-sensitive Fc-targeting antibody fragment labels antibody of choice for analysis of antibody internalization.
Incucyte® Human Fabfluor-pH Orange
Antibody Labeling Dye

One vial: 50 µg

4812

SX5

Incucyte® Human Fabfluor-pH Red
Antibody Labeling Dye

One vial: 50 µg

4722

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Mouse IgG1 Fabfluor-pH Red
Antibody Labeling Dye

One vial: 50 µg

4723

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Mouse IgG2a Fabfluor-pH Red
Antibody Labeling Dye

One vial: 50 µg

4750

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Mouse IgG2b Fabfluor-pH Red
Antibody Labeling Dye

One vial: 50 µg

4751

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Rat Fabfluor-pH Red Antibody
Labeling Dye

One vial: 50 µg

4737

SX5 (configured with Green/Red
Optical Module), S3, SX1

Novel fluorescently tagged Fc-targeting Fab fragments label your antibody of choice for cell surface protein expression.
Incucyte® Mouse IgG2a Fabfluor-488
Antibody Labeling Dye

One vial: 50 µg

4743

SX5, S3, SX1

Incucyte® Mouse IgG2b Fabfluor-488
Antibody Labeling Dye

One vial: 50 µg

4744

SX5, S3, SX1

Incucyte® Mouse IgG1 Fabfluor-488
Antibody Labeling Dye

One vial: 50 µg

4745

SX5, S3, SX1

Incucyte® Mouse IgG1 Fabfluor-555
Antibody Labeling Dye

One vial: 50 µg

BA-04873

SX5

Incucyte® Mouse IgG1 Fabfluor-594
Antibody Labeling Dye

One vial: 50 µg

4844

SX5 (configured with a Green/Red
Optical Module), S3, SX1

Incucyte® Mouse IgG2a Fabfluor-594
Antibody Labeling Dye

One vial: 50 µg

BA-04863

SX5 (configured with a Green/Red
Optical Module), S3, SX1

Ordering Information

Spheroid Growth, Invasion
and Immune Cell Killing
The Incucyte® Spheroid Analysis Software Module
is designed to acquire and analyze label or label-freespheroids to automatically monitor and quantify theformation, growth, shrinkage and invasive propertiesof advanced cell models in real time inside your tissue
culture incubator.

--

Product
Spheroid Software

Description

Incucyte® Spheroid Analysis Software
ModuleFlexible acquisition mode to enable studies of both
single and multi-spheroid assays
Enable long-term imaging with enhanced depth of focus
brightfield (DF® Brightfield) image acquisition
Analyze relevant spheroid metrics using intuitive
Incucyte® processing definitions*Nuclear Lentivirus
Labeling Reagents

Incucyte® Nuclight Green Lentivirus(puro)
Incucyte Nuclight Red Lentivirus(puro)®

Incucyte Nuclight Red Lentivirus(bleo)®

Apoptosis Plasma
Membrane Integrity
Reagents

Quantify and visualize immune cell-mediated killing of solid tumors inreal time using non-perturbing reagents and purpose-built, integrated
Incucyte® Spheroid Analysis Software Module.
SKOV3-NR

BT-474-CyG

MCF7-NR

Cytoplasmic DyeLabeling Reagents+ Herceptin (1 µg mL)

1 module

9600-0019

SX5, S3, SX1

0.2 mL4624

SX5, S3, SX1

0.6 mL

4475

SX5, S3, SX1

0.2 mL

4625

0.6 mL

4476

SX5 (configured with Green/Red
Optical Module), S3, SX1

0.2 mL

4626

SX5, S3, SX1

0.6 mL

4477

SX5, S3, SX1

0.2 mL

4627

SX5 (configured with Green/Red
Optical Module), S3, SX1

0.6 mL

4478

Incucyte® Nuclight Orange Lentivirus(puro)

0.2 mL

4771

SX5

Incucyte® Nuclight NIR Lentivirus
Reagent (puro)

0.2 mL

4805

SX5

Membrane impermeable, highly-selective phosphatidylserine (PS) cyanine fluorescent dyes label PS exposed on theextracellular surface of cells undergoing apoptosis.
Incucyte® Annexin V Green Dye

One vial: 100–
200 tests

4642

SX5, S3, SX1

Incucyte® Annexin V Red Dye

One vial: 100–
200 tests

4641

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Annexin V Orange Dye

One vial: 100–
200 tests

4759

SX5

Incucyte® Annexin V NIR Dye

One vial: 100–
200 tests

4768

SX5

Live-cell cytoplasmic labeling dyes that freely pass through cell membranes and into cells, where they are transformed
into a cell membrane-impermeant form, providing spatial context for cell-to-cell interactions.
Incucyte® Cytolight Rapid Green Dye

One vial: 15 μg

4705

SX5, S3, SX1

Incucyte Cytolight Rapid Red Dye

Five vials: 50 μg

4706

SX5 (configured with Green/Red
Optical Module), S3, SX1®

Cytoplasmic
Lentivirus Labeling
Reagents

Instrument Compatibility

Lentivirus reagents provide homogenous expression of a nuclear-restricted fluorescent protein without altering cellfunction for live-cell quantification of cell proliferation and viability.

Incucyte® Nuclight Green Lentivirus(bleo)

Application Spotlight:
3D Immune Cell Killing

Cat. No.

Analyze growth, viability and invasion of single spheroids in round-bottom multi-well formats or measure multiple
spheroids in flat bottom plates to detect changes in growth and viability.

Lentivirus reagents provide homogenous expression of a fluorescent protein without altering cell function for live-cell
quantification of spheroid growth and shrinkage.
Incucyte® Cytolight Green Lentivirus(puro)

0.6 mL

4481

SX5, S3, SX1

Incucyte® Cytolight Red Lentivirus(puro)

0.6 mL

4482

SX5 (configured with Green/Red
Optical Module), S3, SX1* Pre-labeled Nuclight cell lines are also available for purchase.
Please visit www.sartorius.com/shop for more information.

+ Herceptin (1 µg mL) + PBMCs
Tumor cells either stably expressing Incucyte® Nuclight Red Lentivirus (SKOV3-NR, MCF7-NR) or Incucyte® Cytolight Green Lentivirus (BT-474CyG) were seeded on a bed of Matrigel® in flat bottom 96-well plates. Multi-spheroids were allowed to form (3 d) prior to addition of freshly isolated
PBMCs (E:T, 5:1) and Herceptin. Incucyte® BF and fluorescence images (7 d; SKOV3-NR, MCF7-NR or 10 d; BT-474-CyG) compare the effect ofHerceptin on spheroid proliferation in absence (top panel) and presence (bottom panel) of PBMCs (BF outline mask shown in yellow).

Find out more: www.sartorius.com/incucyte

The Incucyte® Organoid Analysis Software Module
enables you to standardize your entire organoid workflow,from generation, maintenance (Organoid Culture QC) or
access treatment effect on organoid growth (Organoid
Assay) and analysis, using image-based, label-freemeasurements.

Application Spotlight: Organoid Assay--

Track and standardize the analysis of organoids embedded in Matrigel® to generate real-time,physiologically relevant analysis of organoids to better predict therapeutic outcomes using theIncucyte® Organoid Analysis Software Module.
Locate and analyze Matrigel® embedded organoids
automatically
Perform continuous label-free analysis in physiologically
relevant conditions
Use quantitative data to support and document
passaging decisions or probe the effects of treatments
through unbiased assessment of size, count andmorphologyA.

Staurosporine (1 µM)

Intestinal
Organoidst = 10 Days
Post
Treatment

12

Control(DMSO, 0.1%)

96

Staurosporine(1 µM)

3
0

0 48 96 144 192 240
Time (h)Hepatic
Organoidst = 2 Days
Post
Treatment

BF Count (1/Image)Hepatic Organoids

Count

Hepatic
Organoids

Whole Dome

80
70

Control(DMSO, 0.1%)60
50

Staurosporine(1 µM)

40
0 24 48 72 96
Time (h)Eccentricity

Darkness

0.65

80

0.60
0.55
0.50
0.45

Staurosporine(1 µM)
Control(DMSO, 0.1%)

0.40
0 24 48 72 96
Time (h)Organoid Darkness

B.

Avg. Eccentricity

Evaluate multiple organoid types using image-based, kinetic
analysis and characterize differentiation and maturation oforganoids in Matrigel® domes in 24- or 48-well plates.
Pancreatic Organoids

Total Brightfield
Area x 104 µm2

20Application Spotlight:
Organoid Culture QC

Intestinal Organoids

Control (DMSO, 0.1%)

Total Brightfield
Area x 104 µm2

Organoid Culture QC
and Organoid Assay

Control(DMSO, 0.1%)

15
10

Staurosporine(1 µM)

5
0

0 24 48 72 96
Time (h)Zoomed-in BF Images

Staurosporine(1 µM)

75
70

Control

65
60
55

Control (DMSO, 0.1%)

0 24 48 72 96
Time (h)Staurosporine

Brightfield images (A) show mouse intestinal and hepatic organoids (1K cells/well) were embedded in Matrigel® (50%) in 96-well plates andallowed to form organoids for 3 days prior to treatment (vehicle or staurosporine; SSP). Corresponding time-courses of BF area demonstrate
the continued growth of vehicle-treated organoids and the inhibitory effects of SSP across both cell types. SSP-treated organoids losedistinctive rounded phenotype (increased eccentricity) and increase in darkness over time (B).

Zoomed-in+ BF
MaskedOutline75

Intestinal
Organoids

50
25
0

0

24 48 72 96 120
Time (h)75

Pancreatic
Organoids

50
25
0Ordering Information

100

0

24 48 72 96 120
Time (h)Organoid Object Total BF
Area x 104 (µm2)

100Organoid Object Total BF
Area x 104 (µm2)

Organoid Object Total BF
Area x 104 (µm2)

100Product

75

Hepatic
Organoids50

0

24 48 72 96 120
Time (h)Mouse intestinal, pancreatic and hepatic organoids were embedded in Matrigel® (50 or 100%) domes in 24-well plates and imaged on an
Incucyte®. Organoids were automatically located, imaged and over 6–8 days using the Incucyte® Organoid Analysis Software Module. Timecourse profiles and brightfield images show cell type-specific organoid differentiation, morphology and growth.

Description

Cat. No.

Instrument Compatibility

Characterize the differentiation and maturation of organoid cultures in 24- or 48-well plates and assess
treatment effects on organoid growth in 96-well microplates.
Incucyte® Organoid Analysis
Software Module

250Organoid Software

1 module

9600-0034

SX5, S3, SX1

--Study the model of your choice over time with specificand sensitive reagents paired with simple mix-and-read
96 / 384-well protocols
Quantify antibody-dependent cellular phagocytosis(ADCP) with the addition of antibodies (e.g., anti-CD-47)
Confirm phagocytosis signals using HD phase-contrast
and fluorescent images

Cytochalasin D IC 50

0h

Total Fluorescent Object
Area (x 104 mm²/image)

Time-lapse visualization of iPSC-derived microglia (Axol BioSciences)engulfing pHrodo® Orange-labeled apoptotic Neuro-2A (N2A) cells.
Images verify the entry of an apoptotic target cell into the cytoplasmof the microglia. Inhibition of this engulfment by cytochalasin D wasquantified based on orange fluorescence object area over the courseof the experiment.

12 h

Vehicle

20
15
10
5
0
-8

-7 -5 -6log (M)

Inhibition of Efferocytosis With
Cytochalasin D

Microglia

4h

20

Control

15

0.1 µM10

0.3 µM

500

4

8 12 16
Time (h)

1 µM
3 µM
10 µM
20 24

0h

4h

12 h

pHrodoBioparticles®

pHrodo Cell
Labeling Kits®

Description

Cat. No.

Instrument Compatibility

Fluorescent dyes label whole cells with a pH-sensitive fluorophore for real-time analysis of efferocytosis and antibody
mediated cellular phagocytosis.
pHrodo® Orange Cell Labeling Kit
for Incucyte®

One kit

pHrodo® Red Cell Labeling Kit
for Incucyte®

One kit4766

SX5
SX5 (configured with Green/Red
Optical Module), S3, SX1

4 x 1040

0

4

8 12 16 20 24
Time (h)1.2 x 105

Cytochalasin D
Lantruculin A
LY294002

8 x 104
4 x 1040-8-6-4log [Drug] (M)Description

Cat. No.

Instrument Compatibility

Sterile fluorogenic reagents ideally suited to a simple mix-and-read, real-time live cell quantification of phagocytosis.
pHrodo® Red E. coli Bioparticles® for Incucyte®

One vial: 2 mg

4615

SX5 (configured with Green/Red
Optical Module), S3, SX1

pHrodo® Green E. coli Bioparticles® for Incucyte®

One vial: 2 mg

4616

SX5, S3, SX1

pHrodo® Red Zymosan Bioparticles® for Incucyte®

One vial: 1 mg

4617

SX5 (configured with Green/Red
Optical Module), S3, SX1

pHrodo® Green Zymosan Bioparticles® for Incucyte® One vial: 1 mg

4618

SX5, S3, SX1

pHrodo Red S. aureus Bioparticles for Incucyte

One vial: 2 mg

4619

SX5 (configured with Green/Red
Optical Module), S3, SX1

pHrodo® Green S. aureus Bioparticles® for Incucyte® One vial: 2 mg

4620

SX5, S3, SX1

®

4649

8 x 104

30 μM
10 μM
3.33 μM
1.11 μM
0.37 μM
0.12 μM
0.04 μM
VehicleBioparticles

Ordering Information
Product

Product

1.2 x 105

J774A.1 murine macrophages phagocytosing pHrodo® Green E. coli Bioparticles® for Incucyte® in the presence of inhibitors. Time-lapse visualizationof cytochalasin D inhibition of Bioparticle® phagocytosis compared to vehicle over 12 h. Time course also shown for cytochalasin D inhibition.
Concentration-response curves at 24 h compare inhibition of phagocytosis by cytochalasin D, lantruculin A, and LY294002.

®

Ordering Information

Green Area (µm2/image; 24 h)

N2AMonitor phagocytosis in real-time with simple
mix-and-read 96- and 384-well protocols
Validate phagocytosis with time-lapse images
and movies
Study the effect of treatments on full timecourse phagocytosis profiles

Application Spotlight:
Phagocytosis of Bioparticles®
Total Fluorescent Object
Area (x 104 mm²/image)Observe and measure efferocytosis over the entire
assay time course using your choice of target cells
labeled with pHrodo® Orange Cell Labeling Dye for
Incucyte®.

--

Green Object Area (µm2/image)

Application Spotlight:
Efferocytosis Assay

pHrodo® Bioparticles® for Incucyte® are pH-sensitive
conjugated probes specifically formulated for analysisof the internalization of bacterial Gram-positive, Gramnegative or yeast-derived bioparticles by immune cells.

30 μM Cytochalasin D

pHrodo® Cell Labeling Kits for Incucyte® are formulated
for long-term and sensitive analysis of phagocytosis ofdead, apoptotic or antibody-targeted cells over time.

Phagocytosis

Cytochalasin D

Efferocytosis®

®

Incucyte® novel, live-cell labeling reagents and purposebuilt software quantify long-term changes in neurite
dynamics and neuronal activity that enable continuous
analysis of sensitive neuronal cell models.

Application Spotlight:
Neuronal Activity Assay

Application Spotlight: Neurite Dynamics Assay--

Generate kinetic, image-based and automatedmeasurements using Incucyte® Neurotrack Analysis
Software Module for continuous analysis of neurite
outgrowth and stability—inside your incubator.
Conduct long-term studies of neuronal function withnovel, non-perturbing fluorescent reagents
Capture transient events in your choice of cell modelwith non-invasive, repeated measurements of the sameneuronal culture in physiologically relevant conditions
Analyze relevant morphological and functional metrics
using intuitive, purpose-built Incucyte® software

Neurite Length in Response to Glutamate

Incucyte® rCortical Neurons transduced with Incucyte® Neurolight
Orange Lentivirus labeled cells cultured in the presence of Incucyte®
rAstrocytes were treated with glutamate at Day 10. Time course analysisof orange fluorescence neurite length reveals concentration-dependent
treatment effects.

Neurite Length (mm/mm²)

Neurite Dynamics
and Neuronal Activity

250
Control
0.3 µM
3 µM

200

30 µM

150
100

300 µM

50
0

0

2

4

6
8
Time (d)

10

12

14

Incucyte® rCortical Neurons

Access complex, neuronal activity and connectivity
measurements from thousands of cells chronically to gain
unprecedented functional insight into neuronal cell models
using our novel Incucyte® Neuroburst Orange Lentivirus
and Incucyte® Neuronal Activity Analysis Software Module.

Day 2

Product

Incucyte rCortical Neurons and iCell GlutaNeurons (Cellular Dynamics
International) express the Incucyte® Neuroburst Orange Lentivirus,
without perturbing the health and morphology of the cells.

Day 12
Intensity (OCU)
Active Objects = 2003
Mean Correlation = 0.864
4.8
4.2
3.6
3.0
2.4
1.8
1.2
0.6
0
0 20 40 60 80 100 120 140 160 180
Time (s)Day 4Intensity (OCU)
Active Objects = 118
Mean Correlation = 0.0161
4.8
4.2
3.6
3.0
2.4
1.8
1.2
0.6
0
0 20 40 60 80 100 120 140 160 180
Time (s)Day 19Intensity (OCU)
Active Objects = 2028
Mean Correlation = 0.828
4.8
4.2
3.6
3.0
2.4
1.8
1.2
0.6
0
0 20 40 60 80 100 120 140 160 180
Time (s)Description

0.75

6

SoftwarePurpose-built acquisition and analysis software for the detection of calcium oscillations in 96-well plates.

0.25
0.00

0

5

10
Time (d)1520Kinetic quantification (graph above) of
longitudinal, dynamic changes in neuronal
activity (mean burst rate and mean correlation)of iCellGluta Neurons expressing Neuroburst
Orange Lentivirus, showing changes over time
during neuronal network maturation. Active
object traces (corner traces) provide detailed
insight into the dynamic changes in neuronal
activity and connectivity for every acquired
time point.

9600-0032

SX5

Incucyte® Neuroburst Orange Lentivirus

One vial: 2 mL

4736

SX5

Incucyte Neuroactive Orange Kit

One kit4761

SX5

Neurite Dynamics

Characterize neurite dynamics over time in mono- or co-culture models while assessing cell viability (refer to page 7,
Annexin V Reagents)

SoftwareEnables label-free or fluorescent analysis of neurite outgrowth, maturation and disruption in each well of a 96- or
384-well plate.
Incucyte® Neurotrack Analysis
Software Module

30

1 module

Fluorescently detect changes in activity using a novel genetically-encoded fluorescent calcium indicator.

®

0.50
Burst Rate

Instrument Compatibility

Record activity from over a thousand cells to study changes in neuronal network activity and connectivity.

Neuronal Activity
Reagents
Mean Burst
Rate/min9
Correlation

Cat. No.

Neuronal Activity

Incucyte® Neuronal Activity Analysis
Software Module

Mean
Correlation
1.00

Day 10Ordering InformationiCell GlutaNeurons

®

Day 7
Intensity (OCU)
Active Objects = 1363
Mean Correlation = 0.493
4.8
4.2
3.6
3.0
2.4
1.8
1.2
0.6
0
0 20 40 60 80 100 120 140 160 180
Time (s)Day 6

Neurite Labeling
Reagents

Neuronal Cells

1 module

9600-0010

SX5, S3, SX1

Lentivirus reagents driven off a synapsin promoter provide homogenous expression of a fluorescent protein in target
cells without altering cell function for live-cell quantification of neurite outgrowth.
Incucyte® Neurolight Orange Lentivirus

Two vials:
0.45 mL each

4808

SX5

Incucyte® Neurolight Red Lentivirus

Two vials:
0.45 mL each4807

SX5 (configured with Green/Red
Optical Module), S3, SX1

Incucyte® Neuroprime Orange Kit

One kit

4760

SX5

Incucyte® Neuroprime Red Kit

One kit4585

SX5 (configured with Green/Red
Optical Module), S3, SX1

Ready-to-use cryopreserved cells from the cortex of Sprague Dawley rats at Day 18 of gestation.
Incucyte® rCortical Neurons

One vial:
2 x 106 cells

4753

Assay dependent

Incucyte® rAstrocytes

One vial:
2 x 106 cells

4586

Assay dependentApplication Spotlight: Incucyte® Chemotaxis Assay

Incucyte® Scratch Wound and Chemotaxis Assays allow
you to continuously monitor and analyze migrationand invasion using purpose-built consumables andautomated software.

--

Maximize your productivity and insight into dynamic cell movement with automated,label-free imaging and analysis using optically clear Incucyte® Clearview 96-well
Chemotaxis Plates and Incucyte® Chemotaxis Analysis Software Module.
Unlock your productivity with optimized protocols,
peripherals and automated analysis
Confirm cell movement and morphology with images
and movies—all from inside your incubator
Perform reproducible 96-well kinetic migration orinvasion assays using the cell model of your choice—adherent or non-adherent

Chemotaxis of Primary T Cells to SDF-1α
1.2
Total T Cell Area
Normalized to Initial Value

Invasion and
Migration Assays

Application Spotlight:
Scratch Wound Migration and Invasion Assay

Control
3.125 nM
6.25 nM
12.5 nM

0.8

25 nM
50 nM
0.4

200 nM
100 nM

0.0

0

10

20

30

Time (h)Incucyte® Clearview 96-well Chemotaxis Plates provide an optically clear surface for label-free imaging and analysis of chemotactic cell migration
or invasion. Cells are added to the upper chamber and chemoattractant to the lower reservoir plate. Chemotactic transmembrane migration is
automatically quantified as the cells migrate through laser etched pores (yellow circles) toward chemoattractant. Example data of concentrationdependent SDF-1α mediated chemotaxis of CD3/CD28 activated human T cells.

Make label-free, quantitative measurements of migration and invasion
while visualizing morphological changes due to treatment effects, using the
integrated Incucyte® Scratch Wound Analysis Software Module.

Ordering Information
Product
t=0

t=2

t=6

Incucyte® 96-well Woundmaker Tool creates 96 precise,uniform cell-free zones with the touch of a button in cellmonolayers cultured in our Incucyte® Imagelock Plates.
Wound closure is visualized and analyzed in real-time with
the Incucyte® Scratch Wound Analysis Software Module.

Migration

Scratch Wound
Cell Migration andInvasion

Description

Incucyte® Scratch Wound Analysis
Software Module

1 module

9600-0012

SX5, S3, SX1

Incucyte® 96-well Woundmaker Tool

1 wounder

4563

SX5, S3, SX1

Incucyte Cell Migration Kit

Woundmaker Tool
2 Rinse boat assemblies
15 Imagelock Plates
1 Incucyte® Microplate Tray
AlconoxBA-04858

SX5, S3, SX1

Incucyte® Cell Invasion
Accessories Kit

2 Biocision Coolbox
Microplate Systems
3 Coolsink XT96F
1 Biocision Coolbox kit

4444

SX5, S3, SX1

Incucyte® Imagelock 96-well Plate

Pack of 10 plates

BA-04856

SX5, S3, SX1

Pack of 50 plates

BA-04857

SX5, S3, SX1

Invasion

Invasion

MCF-7
Microplate graph showing progression of differential cell migration and invasion of three different cell types using the Relative Wound Density metric.
Representative images of HT-1080 fibrosarcoma cells highlight differences in morphology of cells during migration versus invasion.

Chemotaxis Cell
Migration andInvasion

Instrument Compatibility

Analyze 96-well Incucyte Scratch Wound Assay images to analyze cell migration and invasion with or without labels.

®

Migration

Cat. No.

®

Track and quantify label-free and fluorescently labeled chemotaxis cell migration and invasion in microplate format.
Incucyte® Chemotaxis Analysis
Software Module

1 module

9600-0015

SX5, S3, SX1

Incucyte® Clearview 96-well
Chemotaxis Plates

1 plate4582

SX5, S3, SX1

Pack of 10 plates

4648

SX5, S3, SX1

Incucyte® Nuclight Cells
Bridge the gap between single, target-focusedbiochemical assays and lower-throughput cell-based
follow-up experiments for the identification of novel
treatments
Enhance productivity by evaluating multiple compounds
or treatments in a 96-well format, from any time point
Gain deeper insight into treatment effects throughcoupling long-term evaluation of protein kinase
activity with effects on cell proliferation

Application Spotlight:
Monitoring Kinase Akt Activity

Nuclear Translocation Ratio(Normalized to t = 0)A.

Generate kinetic, image-based and automatedmeasurements of kinase Akt activity using the Incucyte®
Kinase Akt Green/Red Lentivirus to express a stable,
genetically-encoded reporter in a variety of cell types.

1.0
VehicleControl

0.8

0.6
Treatment
Addition

0.4
0

10

20 30 40
Time (min)MK2206

50

60

Post Akt Inhibitor Addition (3 µM MK2206)
Pre-compound

4 Minutes

16 Minutes

Vehicle0 Days

Vehicle3 DaysCB-8393 DaysSelect your pre-labeled cell line of choice, as well as
the fluorescent protein and antibiotic selection
Validated to confirm that Nuclight expression does
not interfere with cell replication
Tested to confirm that the number of residual viralparticles is below detectable limits (GP120 assay) andcertified mycoplasma-free by ATCC—can be used in lowlevel safety category 1

B.

C.

AU565 Proliferation
2000

MDA-MB-231 Proliferation
2000

1500
1000
Vehicle500
CB-839 (1 µM)

0
0

24

48 72
Time (h)1500
Vehicle1000
500
CB-839 (1 µM)

0

96

0

24

48

72

96

Time (h)Fluorescent labeling prior to forming a
co-culture. An equal number of AU565Nuclight Green and MDA-MB-231-Nuclight
NIR were seeded into a 96-well plate andtreated with a glutaminase inhibitor CB-839(1 µM). Phase and fluorescence images
were collected in an Incucyte® Live-Cell
Analysis System over 4 days. Images of green
and NIR fluorescence (A) show that undernormal conditions both cell types proliferate,
while in the presence of CB-839, fewer NIR
nuclei are observed. AU565 cell growth wasminimally affected (B), while MDA-MB-231
cell count was reduced by the compound(C). AU565 cells have a slower growth ratecompared to MDA-MB-231 cells.

Ordering Information

28 Minutes

Product
Incucyte
Nuclight
Cells®

Description

Cat. No.

Instrument Compatibility

One cryopreserved vial of cells expressing Incucyte Nuclight Lentivirus (nuclear-labeled fluorescent protein) at a density of
1 or 2 x 106 cells/vial. Each vial contains a stable population of cells expressing the Incucyte® Green/Red/Orange/NIR fluorescentprotein restricted to the nucleus. Upon arrival, cells should be stored at -150° C or in liquid nitrogen.
®

Incucyte® Nuclight Green A549 Cells

1 mL cryopreserved vials (1 x 106 cells/mL)

4492

SX5, S3, SX1

Incucyte Nuclight Red A549 Cells

1 mL cryopreserved vials (1 x 10 cells/mL)

4491

SX5, S3, SX1

Incucyte Nuclight Orange A549 Cells

1 mL cryopreserved vials (1 x 10 cells/mL)

BA-04864

SX5, S3, SX1

Incucyte® Nuclight NIR A549 Cells

1 mL cryopreserved vials (1 x 106 cells/mL)

BA-04865

SX5, S3, SX1

Incucyte Nuclight Red HT-1080 Cells

1 mL cryopreserved vials (1 x 10 cells/mL)

4485

SX5, S3, SX1

Incucyte Nuclight Green HT-1080 Cells

1 mL cryopreserved vials (1 x 10 cells/mL)

4486

SX5, S3, SX1

Incucyte® Nuclight Green MCF-7 Cells

1 mL cryopreserved vials (1 x 106 cells/mL)

4528

SX5, S3, SX1

Incucyte Nuclight Red MCF-7 Cells

1 mL cryopreserved vials (1 x 10 cells/mL)

4524

SX5, S3, SX1

Incucyte® Nuclight Green HeLa Cell

1 mL cryopreserved vials (1 x 106 cells/mL)

4490

SX5, S3, SX1

Incucyte Nuclight Red HeLa Cells

1 mL cryopreserved vials (1 x 10 cells/mL)

4489

SX5, S3, SX1

Incucyte Nuclight Green Neuro-2a Cells

1 mL cryopreserved vials (1 x 10 cells/mL)

4511

SX5, S3, SX1

Incucyte® Nuclight Red Neuro-2a Cells

1 mL cryopreserved vials (1 x 106 cells/mL)

4512

SX5, S3, SX1

Fluorescently evaluate changes in kinase Akt activity using a novel genetically-encoded kinase translocation indicator

Incucyte Nuclight Red Jurkat Cells

1 mL cryopreserved vials (1 x 10 cells/mL)

4613

SX5, S3, SX1

Incucyte® Kinase Akt Green/Red Lentivirus One vial: 0.2 mL

Incucyte® Nuclight Mix HT-1080/A549
Cells (Green/Red)

1 vial of HT-1080 and A549 cells (1:1) at a
total density of 1 x 106 cells/vial)

4516

SX5, S3, SX1®
®

SK-OV-3 cells stably expressing the Incucyte Kinase Akt Green/Red indicator were treated with Akt selective inhibitor MK2206, resulting intranslocation of the green fluorescent sensor from the cytoplasm to the nucleus. Time course analysis shows a decrease in the nuclear translocation
ratio over time, indicating Akt inhibition.
®

®
®

®

Ordering Information
Product
Kinase Translocation
Reporter--

The Incucyte® Nuclight labeled cell lines are ideal for use
in cell counting and proliferation studies and can be easily
multiplexed with other Incucyte® Cell Health reagents for
simultaneous measurements of apoptosis and cytotoxicity
in monoculture or co-culture models.

NIR Nuclei Count/Image--The Incucyte® Kinase Activity Assay enables users tomeasure and analyze dynamic changes in Akt activity inlive cells in physiologically relevant environments.

Green Nuclei Count/Image

Kinase Activity Assays®
®

Description

Cat. No.
BA-04868

Instrument Compatibility
SX5 (configured with Green/Red
Optical Module), S3, SX1

®

6
6

6
6

6

6
6

6

From our workhorse, theIncucyte® S3, to our economical
Incucyte® SX1, to the newest
member, the frontier-breaking
Incucyte® SX5, we deliver real-timeinsights to simplify progress forlabs of any size.

Incucyte Software Modules®

Incucyte® software makes the process of acquiring,viewing, analyzing and sharing images of living cells easierthan ever before. Using the base software’s purpose-built
analysis tools and comprehensive software modules thatenable powerful phenotypic cellular analysis, researchteams are able to convert images into insight.

Ordering Information
Software Module

Description

Cat. No.

Incucyte® AI Cell Health
Analysis Software Module

Simplify the complexity of classifying live versus dead cells using artificial intelligence (AI)-driven
image analysis without the use of labels. Requires a GPU co-processor installed as a drop-in
hardware upgrade to the controller and Incucyte® Software v2022B or newer software version.

BA-04871

Incucyte® AI Confluence
Analysis (update for Incucyte®
Base Analysis Software)

AI-driven analysis provides a simple workflow for highly accurate segmentation of cells inphase-contrast images, adapting to a wide range of cell types and morphologies with minimaluser input. Incucyte® AI Confluence Analysis is available with Incucyte® Software v2022A ornewer software version.

Part of
Incucyte®
Base software

Incucyte® Advanced
Label-Free Classification
Analysis Software Module

Perform counts and track changes in adherent cell morphology via label-free image
segmentation and multivariate analysis of cell shape. Classifier is trained using control wells.
The Incucyte® Advanced Label-Free Classification Software Module is an add-on and requires
Incucyte® Cell-by-Cell Analysis Software Module (Cat. No. 9600-0031).

BA-04867

Incucyte® Cell-by-Cell
Analysis Software Module

Perform label-free cell counts and subsequent cell-by-cell classification based on shape, sizeor fluorescence intensity to quantify dynamic changes in cell subsets within heterogeneous
living cell cultures.

9600-0031

Incucyte® Organoid Analysis
Software Module

Characterize the differentiation and maturation of organoid cultures in 24- or 48-well plates
and assess treatment effects on organoid growth in 96-well microplates.

9600-0034

Incucyte® Spheroid Analysis
Software Module

Analyze growth, viability and invasion of single spheroids in round-bottom multi-well formats
or measure multiple spheroids in flat-bottom plates to detect changes in growth and viability.

9600-0019

Incucyte® Neuronal Activity
Analysis Software Module

Purpose-built acquisition and analysis software for the detection of calcium oscillations in
96-well plates. For use with the Incucyte® SX5 configured with the Green/Orange/NIR
Optical Module.

9600-0032

Incucyte® Neurotrack
Analysis Software Module

Enables label-free or fluorescent analysis of neurite outgrowth, maturation and disruption ineach well of a 96- or 384-well plate.

9600-0010

Incucyte® Chemotaxis
Analysis Software Module

Track and quantify label-free and fluorescently-labeled chemotactic cell migration andinvasion in microplate format.

9600-0015

Incucyte® Scratch WoundAnalysis Software Module

Automated detection and quantification of wounds from each well of a 96-well plate in orderto analyze cell migration and invasion with or without labels.

9600-0012

Incucyte® ATP Analysis
Software Module

Enables analysis of ATP dynamics by capturing fluorescent images while qualitatively monitoring
associated changes in cell morphology in each well of a 96- or 384-well plate. For use with theIncucyte® SX5 configured with the Incucyte® SX5 Metabolism Optical Module.

9600-0033

Incucyte® Angiogenesis
Analysis Software Module

Identify and analyze the formation and disruption of endothelial vascular networks over timein each well of a 96-well plate.

9600-0011

Analyze your cells for days, weeks or even months as
they sit stationary in the stable environment of yourtissue culture incubator.
With the Incucyte® user-friendly interface and robust
instrument portfolio, any cell biologist can gain
dynamic insights into the health, morphology,
movement and function of their cell models.

Support an entire research team via concurrent, remote
network access. Make real-time decisions from the comfort
of your office or home.

North America
Sartorius Corporation
300 West Morgan RoadAnn Arbor, Michigan 48108
USA
Phone +1 734 769 1600
Email: lps.opm.na@sartorius.com

EuropeSartorius UK Ltd.
Longmead Business Centre
Blenheim Road
Epsom
Surrey, KT19 9QQ
United Kingdom
Phone +44 1763 227400
Email:
orderhandling.lps.ne@sartorius.com

Asia Pacific
Sartorius Singapore Pte. Ltd.
30 Pasir Panjang Road,#06-32, Mapletree Business City
Singapore 117440
Phone +65 6872 3966
Email: sartoriusap@sartorius.com

Find out more: www.sartorius.com/incucyte
For questions, email: AskAScientist@sartorius.com
Specifications subject to change without notice. ©2023. All rights reserved. All names of Sartorius products are registered trademarks and the propertyof Sartorius AG and/or one of its affiliated companies. Incucyte-Reagents-Consumables-Brochure-en-L-Sartorius Status: 02 | 2023