Service Datasheet

Easy Plasmid­ServicePlasmid Designed to Cater
To Your Specific Requirements,
Ensuring a Seamless And
Cost-Effective Experience
Service Information
Our plasmid platform offers a range of easy-to-use plasmids
that can provide you with several benefits for your scientificprojects. Based on e-Zyvec® technology, we create a libraryof plasmid template to fit your needs. Use our online platform
to choose the plasmid that best suits you, modify your­expression cassette, and submit your request.
With the help of our experts, get a review of your plasmid­before placing an order, and then receive your ready-to-use
plasmids in the desired quantity for your project.

---Features and Benefits
Cost-Effective: Reduce your project costs by using
easy plasmids (from 249 €)Scalable: Access highly complex plasmids with our­tailor-made service, up to GMP grade
Quality Assurance: Plasmid scientist experts and wholeplasmid sequencing included
Time-Saving: Ready-to-use discovery grade plasmids­within 5 weeks from orderingIntroduction--

Relevant Applications
Fundamental research
Cell and gene therapy: AAV and LV transfection

Quality Grade Provided
Easy plasmid service provides plasmid amplification up toseveral mg of plasmids as discovery grade (Miniprep andwhole plasmids sequencing as QC are included).
Higher quantity and | or quality (up to GMP grade) can be
performed through our manufacturing services.

Easy Plasmid Service Workflow
1. Access: Log in to our online design platform and start a
new plasmid project.
2. Select: Choose an easy plasmid template that meets your­requirements (if any plasmid architecture fits, create a­tailor-made one through our plasmids engineering service,nothing is restricted).
3. Design: Modify the expression cassette by selecting a­promoter and inserting your gene of interest.
4. Review: Submit your design for a complimentary expert
plasmid ­review.
5. Confirm: Approve the final design and specify the ­required
plasmid quantity.
6. Production: Keep updated on the production progressof your plasmids through our online platform.
7. Quality Control: Validation of your plasmid thanks to­different QCs as whole plasmids sequencing.
8. Move Science Forward: Finalize your project with ourready-to-use plasmids.

Termination siteGene of interet

Promoter

2Plasmid ­templateOrdering Information
Easy plasmids have been designed to make the process of plasmids design and production, easier and cheaper (­ from 249 €).
To proceed with the order, create an account at www.polyplus-sartorius.com/register, then access the design platform.
Create your project and choose your easy plasmids, then submit your plasmids project. It will be reviewed by our experts,
who will return to you with their suggestions. Then a quote will be issued.
Product Name

Product Description

Order Number

Plasmid pTransgene AAV

Plasmid Transgene to be co-transfected into packaging cells along with the pHelper plasmid(e.g. pPLUS® AAV-Helper), the pRepCap plasmid, and transfection reagents (e.g. FectoVIR®-AAV),­enabling the efficient production of viral particles to further transduce targeted cells | tissues toexpress the gene of interest.

101000221

Plasmid pTransgene LV

Plasmid Transgene plasmid to be co-transfected into packaging cells along with packaging lentiviralplasmids encoding the Gag, Pol, Rev, VSV-G viral components, and with the transfection reagents(e.g. FectoVIR®-LV).

101000220

Plasmid shRNA Expression

The easy shRNA plasmid to base-pairs with a complementary target messenger RNA (mRNA),
leading to its degradation.

101000219

Plasmid Protein Expression

The easy protein expression plasmid engineered by Polyplus carry the genetic features requiredby mammalian systems to synthetize and express the desired protein.

101000218

End Product Information--

We deliver discovery grade plasmids as circular, double
stranded DNA molecules, in a screw-cap plastic tube,­re-suspended in 1 × AE buffer (miniprep.) or 1 × TE buffer(midi-, maxi-, giga- preparation.), at room temperature.
Please note that we do not provide bacterial stock.

StorageUpon receipt, briefly spin down the tubes containing yourengineered plasmids (bench centrifuge) and store them at:
4 °C for short storage (<1 month)−20 °C for long storage

For research-, high quality- and GMP-grade, please refer
to our plasmid manufacturing service.

We strongly recommend users aliquot discovery grade­plasmids for long storage and to avoid freeze-thaw cycles.
Duration of storage is given here as an indication. Always­verify the integrity of plasmids stored for extended periods
by gel electrophoresis (denaturation) or sequencing­(sequence integrity).

----Quality Controls
QCs are limited to basic quality and identity tests:
DNA quantification
A260/280 and A260/230 measurements
Restriction digest profile (gel or capillary electrophoresis)
Whole plasmid sequencing including mycoplasma andgDNA detection (NGS)
Sanger sequencing (if necessary)
QC results will be documented in the Product Delivery Form.
Endotoxins levels are not measured for discovery grade­plasmids.

Intellectual Property
Unless otherwise stated in the specifications of you project,
every engineered plasmid provided on a service based­contract, for Research Application, are IP free from Sartorius,
as stipulated in our T&C. Other applications may require­licensing agreement with Sartorius or tiers entity.

3Germany
Sartorius Stedim Biotech GmbH
August-Spindler-Strasse 1137079 Goettingen
Phone +49 551 308 0USASartorius Stedim North America Inc.
565 Johnson Avenue
Bohemia, NY 11716
Toll-Free +1 800 368 7178

F or more information, visit
sartorius.com/transfection-reagents-plasmids

Specifications subject to change without notice.
©2024 Sartorius Stedim Biotech GmbH, August-Spindler-Strasse 11, 37079 Goettingen, Germany
Status: 03 | 2024

FrancePolyplus®—Now part of Sartorius
75 Rue Marguerite Perey
67400 Illkirch
Phone +33 390 406 180