Service Datasheet

Whole PlasmidSequencing
Service
Full-Length Sequencing ofMultiple DNA Plasmids
Service Information
Our state-of-the-art Whole Plasmid Sequencing Service
provides high-quality and accurate sequencing of any
plasmid, irrespective of size and complexity.
The service delivers rapid results (from three working days
upon receipt of plasmids). Beyond the data, our personalized
consultations and competitive pricing make plasmid
sequencing accessible to all researchers, with the possibility
to sequence a single plasmid sample, or up to 150 samples
in one run.

--Features and Benefits
Time-Saving: Receive your whole plasmid sequencing
results in as little as three working days.
Personalized Reports: Full-length sequencing ofpredictive (sequence matching) or unknown plasmids(de novo analysis)
Expertise: In-house bioinformatical pipeline allowing
quantification and identification of contaminant sequences

Introduction
Our Expertise
We have spent years refining our understanding of plasmid
structures, sequences, and variations. This expertise enables
us to deliver precise insights, including de novo analysis, that
advance your research and applications. Our capabilities
extend beyond data provision; we also partner with you tonavigate the complexities of plasmid-related challenges.
Thanks to our service, you get more than a sequencing
service — you access a depth of plasmid expertise designed
to elevate your scientific journey.

Plasmid Sequencing Workflow
Place order and send sample(s)Sample preparation

Delivery Time
Getting your sequencing results is now faster than ever.
Ordering sequencing for 12 or more plasmids guarantees
the availability of results within three working days of sample
receipt, ensuring rapid progress for your projects.
Alternatively, our standard service delivers comprehensive
results as early as two weeks following sample receipt.
This fast turnaround time allows you to quickly obtain thesequence information you need to move forward with yourresearch projects.

Sequencing by Illumina

Bioanalytics

Results available online2Ordering Information
Our plasmid sequencing services are dedicated to wholeplasmid sequencing. Therefore, the starting material shouldbe purified double-stranded DNA plasmids. We offer
next-generation sequencing for even a single sample, withno upper limit on the number of samples — we can sequence
up to 150 samples simultaneously. Please note that we do
not perform whole genome sequencing of any organism.

---After sending an order through our online platform, you cansend your samples following the guidelines below:
Use a 1.5 mL plastic tube, hermetically closed (screw capor parafilm)
Label the tube clearly
Minimal volume: 15 µL
Minimal concentration: 100 ng/µL
Samples should be sent to:
Polyplus-Transfection
Production Team
80 rue du Dr Yersin
59120 Loos
FRANCEDeliverables-

After sequencing your sample, we will provide the full-lengthsequence of your plasmid (.fasta file) as well as a sequencing
report (.pdf file) containing:
A visual representation of the sequencing results with thecoverage and the differences observed with the sequenceof reference, if there is any
The positions and the nature of the variations (insertion,deletion, substitution) observed in comparison with the­sequence of reference
The percentage of contaminations contained in your­sample, such as bacterial gDNA
The sequencing report and the full-length sequence of yourplasmids are available on your account.

PCMVITR
FireflyWhen known, please enter the sequences of referenceof your samples on our platform.
05,000eZ-Ori

3,00

04,00000

1,02,00

0WPRE

KanR
SV40-PAITRFeatures
Sequencing depth
Differences vs. expected3Germany
Sartorius Stedim Biotech GmbH
August-Spindler-Strasse 1137079 Goettingen
Phone +49 551 308 0USA
Sartorius Stedim North America Inc.
565 Johnson Avenue
Bohemia, NY 11716
Toll-Free +1 800 368 7178

F or more information, visit
sartorius.com/transfection-reagents-plasmids

Specifications subject to change without notice.
©2024 Sartorius Stedim Biotech GmbH, August-Spindler-Strasse 11, 37079 Goettingen, Germany
Status: 03 | 2024

FrancePolyplus®—Now part of Sartorius
75 Rue Marguerite Perey
67400 Illkirch
Phone +33 390 406 180