iQue® 3 and iQue®
Screener Plus
Fluorochrome Chart

Revealing Insight with Multiplexed Panels
Selecting the proper fluorochromes for multiplexing markers of cell phenotype and function in a single well is critical toresolve complex cellular research questions in order to efficiently characterize disease state.

Designing Multicolor Experiments is as Easy as 1, 2, 3:
1.

2.

3.

Know Your Instrument
What are the number and type oflasers available?
What detector | filter combinations
are available to capture the signal?

Know Your Detection Reagents
What laser wavelength excites them?
What is the emission spectra?
Does spillover exist between
adjacent channels?
How bright are they? What is theirstain index?
Beware that tandems and other dyes
can degrade when exposed to light,
temperature changes, or fixation.

Know Your Biology
Reserve the brightest fluorochromes
for the lowest expressed antigens
and vice versa.
Avoid spillover of highly expressed
antigens into detectors requiringhigh sensitivity.

This determines what labeling anddetection reagents are possible to use
on your system and how they will
perform.

This information will allow you todetermine which fluorochrome will be
best matched to which antigen or
marker of interest.

Find out more
www.sartorius.com

2Now, you have enough information tobegin to answer your experimental
questions by optimizing
fluorochromes and dyes for yourbiology on your instrument.

iQue® 3 and iQue® Screener Plus
Blue-Red ConfigurationiQue® reagents are indicated in bold.
LaseriQue® 3 andExcitation iQue® Screener
Plus Fluorescence
Detectors | Filters

Detector

Fluorochromes

FluorescentProteins

Viability

Blue488 nm

BL1FITC

eGFP

iQue® CelliQue® CellMembrane Integrity Proliferation and(B | Green) Dye
Encoding (B | Green)
Dye
Calcein AM

530 | 30

Alexa Fluor 488

eYFP

Horizon® BB515

Emerald®

mNeon Green

Proliferation,Function, andApoptosis

LIVE | DEAD®
Fixable Green
Zombie Green572 | 28

BL2PEiQue® Human
Caspase 3 | 7
ReagentiQue® Human
Annexin V Reagent

PIeFluor 570®

DNA andCell Cycle

Vybrant® Dye
Cycle Green
Click-iT® Plus
EdU Alexa Fluor
488

PIiQue Qbeads®
Plexscreen
SYTOX® Orange
675 | 30BL4PerCP-Cy™5, Cy5.5
PE-Cy5, Cy5.5
eFluor PE-Alexa Fluor
647
PerCp-Vio700®

780 | 60

BL5iQue® Cell
Membrane Integrity(B | Red) DyeiQue® Cell
Cycle Kit
PI7AADPI

PE-Cy7
PE-Vio770®

Red640 nm

675 | 30

RL1iQue® CelliQue® Cell
Membrane Integrity Proliferation and(R | Red) Dye
Encoding (R | Red)Dye
FVD eFluor 660

DRAQ®5LIVE | DEAD®
Fixable Far Red

iQue Qbeads®
Plexscreen

FxCycle Far Red

APC-Alexa Fluor 750

FVD eFluor 780

APC-Cy7LIVE | DEAD
Fixable Near IR

iQue Qbeads®
Plexscreen

APC
Alexa Fluor 647
APC-Alexa Fluor 680

780 | 60

RL2

APC-Vio770®

Click-iT Plus
EdU Alexa Fluor
647

iQue Qbeads®
Devscreen SH, sAV
Vybrant
DyeCycle Ruby

iQue Qbeads®
Devscreen SH, sAV3iQue® 3 and iQue® Screener Plus
Violet-Blue-Red ConfigurationiQue® reagents are indicated in bold.
Laser
iQue® 3 andExcitation iQue® Screener
Plus Fluorescence
Detectors | Filters

Detector

Fluorochromes

FluorescentProteins

Viability

Violet405 nmVL1

Horizon BV421

eBFP

Pacific Blue

AzuriteAlexa Fluor 405

CeruleaniQue® CelliQue® CellMembrane Integrity Proliferation and(V | Blue) Dye
Encoding V | Blue
(Tag-it Violet™) Dye
Calcein Violet

445 | 45

Proliferation,Function, andApoptosis

SYTOX® Blue

CellTrace® Violet

LIVE | DEAD®
Fixable Violet

Click-iT Plus EdUPacific BlueDNA andCell Cycle

DAPI
FXCycle® Violet
Vybrant
DyeCycle Violet

DAPI
530 | 30

VL2

Pacific GreenvGFP

Horizon BV510

LIVE | DEAD®
Fixable Aqua

VioGreen®
572 | 28

VL3

Horizon BV570

LIVE | DEAD®
Fixable Yellow

Qdot® 605
615 | 24

VL4

Horizon BV605
Qdot 625

675 | 30

VL5

Horizon BV650
Qdot 655

Blue488 nm

780 | 60

VL6Horizon BV785

530 | 30

BL1FITCeGFP

Alexa Fluor 488

eYFP

Horizon BB515

EmeraldmNeon GreeniQue® CelliQue® Cell
Membrane Integrity Proliferation and(B | Green) Dye
Encoding (B | Green)
DyeCalcein AM
LIVE | DEAD®
Fixable GreeniQue® Human
Caspase 3 | 7 Reagent

Vybrant Dye
Cycle Green
Click-iT Plus
EdU Alexa Fluor
488

Zombie Green572 | 28

BL2PEeFluor 570

PIiQue® Human
Annexin V Kit
iQue Qbeads®
Plexscreen
SYTOX® Orange615 | 24

BL3Horizon PE-CF594
PE | Dazzle® 594
PE-Texas Red
PE-Alexa Fluor 610

4PI

iQue® reagents are indicated in bold.
LaseriQue® 3 andExcitation iQue® Screener
Plus Fluorescence
Detectors | Filters

Detector

FluorochromesBlue
488 nmBL4

PerCP-Cy5, Cy5.5

675 | 30

PE-Cy5, Cy5.5

FluorescentProteins

Viability

Proliferation,Function, andApoptosis

DNA andCell Cycle

7AAD
PIiQue® Cell Membrane iQue® Cell
Integrity (B | Red) Dye Cycle Kit
PIeFluor PE-Alexa Fluor
647
PerCp-Vio700
780 | 60

BL5PE-Cy7
PE-Vio770

Red640 nm

675 | 30

RL1APC
Alexa Fluor 647
APC-Alexa Fluor 680

iQue® CelliQue® Cell Proliferation DRAQ5
Membrane Integrity and Encoding (R | Red) Click-iT Plus
(R | Red) Dye
Dye
EdU Alexa Fluor
FVD eFluor 660
LIVE | DEAD
Fixable Far Red®

780 | 60

RL2

APC-Alexa Fluor 750

FVD eFluor 780

APC-Cy7LIVE | DEAD®
Fixable Near IR

APC-Vio770

iQue Qbeads®
Plexscreen
iQue Qbeads®
Devscreen SH, sAV
iQue Qbeads®
Plexscreen

647
FxCycle Far Red

Vybrant
DyeCycle Ruby

iQue Qbeads®
Devscreen SH, sAV ind out moreF
www.sartorius.com

5iQue® 3 and iQue® Screener Plus
Violet--Yellow-Blue ConfigurationiQue® reagents are indicated in bold.
Laser
iQue® 3 andExcitation iQue® Screener
Plus Fluorescence
Detectors | Filters

Detector

Fluorochromes

FluorescentProteins

Viability

Violet405 nmVL1

Horizon BV421

AzuritePacific Blue

Cerulean

Horizon V450

eBFP

iQue® CelliQue® Cell
Membrane Integrity Proliferation and(V | Blue) Dye
Encoding V | Blue
(Tag-it Violet™) Dye
Calcein Violet

445 | 45

Alexa Fluor 405

Proliferation,Function, andApoptosis

SYTOX® Blue

Cell Trace Violet

LIVE | DEAD
Fixable Violet

Click-iT Plus EdU
Pacific Blue®

DNA andCell Cycle

DAPI
FXCycle Violet
Vybrant
DyeCycle Violet

DAPI
530 | 30

VL2

Pacific GreenvGFP

Horizon BV510
586 | 20

VL3

Horizon BV570

LIVE | DEAD®
Fixable Yellow

Qdot 605
615 | 20

VL4LIVE | DEAD®
Fixable Aqua

Horizon BV605
Qdot 625

660 | 20

VL5

Horizon BV650
Qdot 655

Yellow
561 nm780 | 60

VL6

Horizon BV785

586 | 20

YL1

PEmStrawberry

Alexa Fluor 546

tdTomato

LIVE | DEAD®
Fixable RediQue® Human
Annexin V Kit

AsRed 2

CellROX® OrangeCellTrace Calcein
Red-Orange AM

mRFP

615 | 20660 | 20

YL2YL3

Horizon PE-CF594

mCherry

Cell Tracker Orange

PE | Dazzle 594

HcRed

Cell Tracker Red

PE-Texas RedmRuby

PE-Alexa Fluor 610

mStrawberry

PE-Cy5, Cy5.5

mPlum

eFluor PE- Alexa Fluor
647
780 | 60

YL4

PE-Cy7
PE-Alexa 750
PE-Vio770

6

SYTOX® Orange

Vybrant
DyeCycle
OrangeClick-iT Plus
EdU Alexa Fluor
594
SYTOX®
AADvanced™
DRAQ7

DRAQ5
Vybrant
DyeCycle Ruby

iQue® reagents are indicated in bold.
LaseriQue® 3 andExcitation iQue® Screener
Plus Fluorescence
Detectors | Filters

Detector

Fluorochromes

FluorescentProteins

ViabilityBlue488 nm

BL1

Horizon BB515

eGFP

FITC

eYFP

Alexa Fluor 488

EmeraldiQue® CelliQue® Cell
Membrane Integrity Proliferation and(B | Green) Dye
Encoding (B | Green)
Dye
Calcein AM

530 | 30mNeon GreenLIVE | DEAD®
Fixable Green

Proliferation,Function, andApoptosis

iQue® Human
Caspase 3 | 7 Reagent

DNA andCell Cycle

Vybrant Dye
Cycle Green
Click-iT Plus
EdU Alexa Fluor
488

Zombie Green615 | 20BL3Horizon PE-CF594
PE | Dazzle 594
PE-Texas Red
PE-Alexa Fluor 610

660 | 20BL4PerCP
PerCp-Cy5, Cy5.5
PE-Cy5, Cy5.5
PerCp-Vio700iQue® Cell
Membrane Integrity(B | Red) DyeiQue® CellCycle Kit
PI7AADPI

The iQue® technology is protected by the following
patents and other patents pending:
6,890,487, 6,878,556, 7,368,084, 7,842,244, 8,021,872,8,268,571, 8,637,261, 8,823,943, 9,012,235, D,722,515
 ind out moreF
www.sartorius.com

7

For Research Use Only. Not for In Vitro Diagnostic Use.
Learn why iQue® is the choice of leaders in Immuno-Oncology, Antibody Discovery and Immune Target Screening.
For more information or a quote, call us at +1 505 345 9075 or email at sales@intellicyt.com

North America
Essen BioScience Inc.
300 West Morgan RoadAnn Arbor, Michigan, 48108
USAPhone: +1 734 769 1600
Email: orders.US07@sartorius.com

EuropeEssen BioScience Ltd.
Units 2 & 3 The Quadrant
Newark Close
Royston Hertfordshire
SG8 5HL
United Kingdom
Phone: +44 1763 227400
Email: euorders.UK03@sartorius.com

Asia Pacific
Sartorius Japan K.K.
4th Floor Daiwa Shinagawa North Bldg.
1-8-11 Kita-Shinagawa
Shinagawa-ku, Tokyo140-0001
Japan
Phone: +81 3 6478 5202
Email: orders.US07@sartorius.com

F or further information,visit www.sartorius.com
Specifications subject to change without notice. ©️ 2020, Essen BioScience, Inc., part of the Sartorius Group. All Rights Reserved. Intellicyt,iQue, iQue3, Forecyt, and all names of Intellicyt products are registered trademarks and the property of Essen BioScience unless otherwise
specified. Intellicyt is a Sartorius brand. Printed in the EU or US on paper bleached without chlorine. Publication No.: 12088
Version E: 12 | 2020