Updated: May 22, 2020

For research use only

ProteoGREEN™-gGlu
Table 1. Product information
Catalog no.
GC801

Product nameProteoGREENgGlu

Amount20 µg × 10

Storage upon receipt

Stability≤−20°C, keep desiccated1 year (when unopenedand protected from light.

and stored as described.)

1. About ProteoGREEN-gGlu
ProteoGREEN-gGlu(gGlu-HMRG)

isanactivable

fluorescentprobe

todetect

expressionof

γ-glutamyltransferase (GGT). gGlu-HMRG does not emit fluorescence until GGT hydrolyzes it. GGT
distributes at cell membrane surfaces and is overexpressed in most cancer cells, so it could be used to detect
cancer cells. A green fluorescent product has high membrane permeability and localizes inside the lysosomes.

absorbance (-GGT)

1

Relative Intensity

absorbance (+GGT)
fluorescence (-GGT)
fluorescence (+GGT)

0.5

0400

450

500

550600

Wavelength (nm)Figure 1. Absorption and emission spectra.
Fluorescence intensity increases 350-fold by GGT■ Storage
Upon receipt, store the product desiccated and protected from light at ≤ −20°C. Please use up the solution
after dissolving the product. Storing as a solution is not recommended.

■ Precautions
Under an acidic environment, ProteoGREEN-gGlu emits fluorescent by the mechanism different from GGThydrolysis. Therefore, please dilute the probe with a neutral or weak basic solution (pH > 7). The probe alsodegrades and generates fluorescent products at high temperature, so use the solution promptly after
dissolving. To store the solution temporarily, keep the aqueous solution on ice or freeze DMSO solution.
Please use the probe rapidly as possible.

2. An example of cell imaging■ Preparation of reagent
1. ProteoGREEN-gGlu is an orange solid. Before opening the vial, spin down the solid to the bottom by amicrocentrifuge or by a desktop centrifuge.
2. Warm the vial to the room temperature and add 29.7 μl of DMSO or PBS to one vial to make 1 mM stocksolution. Dilute the stock solution with a neutral buffer or culture medium and use the diluted solution
immediately.
■ Reaction with cells
1. Dilute 1 mM ProteoGREEN-gGlu with HBSS or RPMI-1640 to 1-2 µM.
2. Replace the cell culture medium with the cell staining solution and incubate at 37 oC, 5% CO2. for 5-30
minutes. A sufficient increase in fluorescent intensity is observed in GGT-highly expressed cells at 5-15
minutes.
3. Since the background is negligible, it is possible to observe fluorescence without any changes. Thefluorescent intensity might increase when enzyme activity proceeds. To prevent such an increase,replace ProteoGREEN-gGlu solution with observation buffer (such as HBSS) and then observe the cells.
However, repeated washing would decrease the fluorescent intensity.
■ Fluorescent observation
The generated fluorophore can be excited by 488 nm laser and emits fluorescence at 525 nm. For
fluorescence microscopy, blue excitation filter cubes for FITC or GFP are suitable.
Table 2. Related Products
Catalog no.

Product name

Major applications

GC6000HMRG

Hydroxymethyl rhodamine green (green fluorescent probe).

A101-01MAR

Detection of cellular hypoxia.

GC301

AcidiFluor ORANGE

SK3001-01

HPF

Detection of hydroxyl radical (・OH) and peroxynitrite (ONOO−).

SK3002-01

APF

Detection of hydroxyl radical ( ・ OH), peroxynitrite (ONOO−) andhypochlorous acid (HOCl).

GC3004-01

OxiORANGE

Detection of lysosomes with orange fluorescence.

Detection of hydroxyl radical (・OH) and hypochlorous acid (HOCl).

GORYO Chemical, Inc.
EAREE Bldg. 5F, Kita 8 Nishi 18-35-100, Chuo-ku, Sapporo 060-0008, JAPANTEL: +81-11-624-5860
https://goryochemical.com/en/