iQue® 3Truly Fast.
Simply Effortless.
High-Throughput Screening(HTS) by Cytometry

iQue® HTS
Cytometry:
Screen a 96-Well
Plate in 5 Minutes

Speed and throughput are essential in cell analysis
workflows. Whether it is screening of therapeutic
antibodies or evaluating chimeric antigen receptor(CAR) T cell function, progress depends on the rapid
evaluation of samples in a way that is biologicallyrelevant, reproducible, and cost-effective.
The iQue® 3 HTS Cytometer is designed to be a data
generation powerhouse in high-throughputscreening workflows. With a focus on speed andease of use from setup to acquisition and analysis, itdelivers high quality results in the time it takes toenjoy a coffee break.
Featuring a patented sampling method, the iQue® 3
allows for the fastest sample acquisition in theindustry. It accommodates 96-, 384-, or 1536-wellplates, and enables continuous plate loading
through integration with any automation system.

Screening
Run high-throughput
screens and generate morebiologically relevant datawith simultaneous analysisof phenotype and cytokines
in every well.
Find out more
For more information, please visit
www.sartorius.com/iQue-products

SpeedRelieve capacity
bottlenecks throughout
your entire workflow withthe fastest plate sampling,
integrated analysis, andnovel data reduction tools.

Miniaturization
Conserve precious
samples and reagents bysampling as little as a single
microliter from miniaturized
assay volumes.

Ease of Use
Enjoy a simple, scalable,
multi-user environment
with walkaway automation
features including 48 hour
uninterrupted run times
and automated QC beadvortexing on-board.

Automation Ready
Take advantage of theopen platform designfor integration withpopular robotic systems
to load | remove plates
and initiate runs.

The iQue® 3:
Now You Can Leave
the Lab on TimeWith unique sample-handling features,
intelligent processing, and automatedtemplate-based analysis, the iQue® 3
gets you from samples to actionable
results faster than ever before.

-The iQue® 3 is a simple, scalable,
multi-user environment featuring
walkaway automation, comprehensive
analysis and visualization tools that are
not available in traditional flow
cytometers.
Walk-up-and-use operation
eliminates the need for pre-analyticalsetup and reduces reliance on ‘powerusers’Patented, rapid microvolume sampling
processes 96-well plates in as few as
5 minutes or 384-well plates in
20 minutes.
The iQue® 3 comes in flexible plate
formats and is suitable for a broad
range of cell and bead-based analyses.

Minimal volume sampling capabilities,
as little as 1 μL, conserve samples andreagent costs.
Find out more
For more information, please visit
www.sartorius.com/iQue-products

Robotic integration capabilities enablehigh throughput applications.

Onboard vortexing just prior tosampling ensures proper
resuspension and delivers improved
QC results and consistency.

Extended run time on cartridges
allows for unattended multiple plateprocessing. Liquid level detection
and user-definable low-level warnings
help ensure there are always enoughreagents to complete planned runs.

Samples are delivered to detectors
in an air-gap delimited stream as partof a patented, rapid microvolume
sampling process.

The iQue 3:
Get to Your Destination Faster®

The iQue® 3 comes in multiple optical configurations and flexible plate formats
to match the diverse needs of researchers. The platform delivers a consistentprocess that translates into time and resource savings, with additional options
like robotic integration, increased fluidic capacity, and centralized data storage
that further extend productivity.

-

1. Simple Assay Setup
Optimized mix-and-read reagent kits with readyto-use analysis templates
Flexible fluorophore options with up to 3 laser
excitation and 13 color emission channels
No adjustment optics for scalable, reliable, andreproducible data collection in multi-userenvironments-

2. Intelligent Hardware Integration
Enhanced rinse station with intelligent software
reporting of cartridge levels
Patented sampling technology processes 96 wells
in as little as 5 minutes; 384 wells in 20 minutes
Automated plate calibration, QC, detector cleaningand shut down

An Integrated Solution
That Supports You Throughthe Entire Workflow-

3. Real-Time Data Acquisition and Analysis
Single-software solution for all your acquisition
and analysis needs
Rapid data visualization—adjust a gate to see
updated plate-wide analysis in real time
Guided creation of metrics, statistics, visualizations
and reports-

4. Dynamic Visualization of Results
Easily identify wells of interest using multiple
selection criteria
Compare, identify, and rank wells in all plates
across your experiment
Drill down from experiment level, to plate level,to well level, to cell level

The Enhanced Rinse Station (ERS):
Improved, Intelligent Fluidics forMore User-Friendly Operation

The iQue® 3 features an enhanced rinse station that
actively monitors buffer usage with built-in liquid
level detection performed by load cells under eachcartridge and offers a smarter way to ensure you
always have enough buffers to complete your run.

-48 hour run time before replacing reagent
cartridges
Extended run time on cartridges forunattended multiple plate processing
Intelligent software monitoring of rinse station
fluid levels
Improved reagent cartridges with lessevaporation and the ability to easily closeand store if needed

Improved Usability with the Enhanced
Rinse Station
Automated vortexing just prior to sampling for
improved QC results and consistency
Routine tasks performed with the touch of abutton, including system cleaning, plate map
calibration, shutdown, and even fluidics
refilling with the Qmax Refill Module
Warnings for missing, low, and even user
defined volume levels

Find out more
For more information, please visit
www.sartorius.com/iQue-products--

Reagent Cartridges
Double the volume and run time of previous
reagent troughs
Easily resealable with cap closure for storage
if needed
Simple to install and replace

Forecyt® Software
Through integration with Forecyt® software, the ERS
minimizes the chances of failed runs due to insufficient
reagent levels.
Intelligent reagent level reporting based on
cartridge weightUser definable low level reminder
Low level warnings

Forecyt Software: What Easy Analysis Looks Like®

One of the key features of the iQue® 3 platform is the Forecyt® Software, which delivers a fully integrated
experience, from data acquisition to plate-level analytics and multi-parametric data visualization.

--

--

--

Number ofUsers

Distributed
ClientAccessVisualize
Link visualizations together torapidly interrogate results
Locate wells and arrays of interest
Compare, identify, and rank wells
across your entire experiment

Number ofInstruments

Analyze
Intuitive workflow with plate
visualizations including heat maps,
whole plate views, dose response, andstandard curves
Interactive gating with real-time results
Easy report generation and data export

Number ofComputers

AcquireAnnotate plate wells for downstream
analysis
Select channels and define sampling,washing, and cleaning routines
Apply analysis templates for
instantaneous multi-plate processing

Standard Edition—a standalone solution that comes with each instrument—
can be used to set up experiments, acquire data and analyze results on thePC attached to the instrument.

OneOneSingleNo

The Offline Edition is ideal for users who want to analyze data away from the laboratory,
without a network connection.

OneNoneSingle

NoThe Enterprise Edition is a secure multi-user, multi-instrument data storage andmanagement solution that provides distributed access to remote analysis capabilities.

Many*

Many*

Multiple*

YesForecyt® Software is Available in Three Configurations—
Scalable to Meet Your Growing Needs:

Applications for the iQue 3®

---

Antibody Discovery andDevelopment
Increase data throughput andquality by multiplexing antibody
binding, function, and titer acrossthe process.	Antibody screening
Functional profiling
Cell line development---

Adoptive Cell Therapy
Assess multiple cell parameters
faster, with fewer cells and lessreagents.	Immune cell killing
Immune cell assessment
Cytokine profiling---Small Molecule Screening
Perform high-content phenotypic
screening of immune biology
across the drug discovery process.
Primary immune cell screening
Yeast and bacterial assays
Target identification with siRNAand CRISPR

Mix-and-Read Screening Kits for Cellular
Phenotype and Function Applications
iQue® kits include optimized reagents that are validated on the iQue® 3 platforms, with flexible formats. No- or onewash protocols save time, reduce variability, and allow for miniaturization. Kits can be multiplexed to increase assay
content and reduce sample requirements.
To view a complete listing of our reagents and consumables visit:https://www.sartorius.com/en/products/flow-cytometry/flow-cytometry-reagents
Bead-Based Kits
Qbeads® Plexscreen
Human Secreted Proteins
Mouse Secreted ProteinsRat Secreted Proteins
Qbeads Human Inflammation
PanelQbeads® Devscreen
SAv (Streptavidin)
SH (Sulfhydryl)
Qpanels® T Helper Kits
Th1/2 4-plex
Th1/2 6-plex
Th1/2 9-plex
Th1/2/17 7-plex

Cell-Based Kits
Cell Assay Kits
Cell Cycle
4-Plex Apoptosis
Singleplex Apoptosis (mix andmatch)
Cell Membrane Integrity
Cell Proliferation and Encoding
Antibody Internalization Kits andReagents (human and mouse)

Cell and Bead-Based Kits
Cell Line Development
Cy-Clone™ PLUS
Antibody Screening
Mouse IgG Type and Titer
Immune Cell Characterization
T Cell Activation
T Cell Memory
T Cell Exhaustion
Mouse T Cell Biology
Immune Cell KillingiQue 3:
The Complete Solution for Faster
Screening by Cytometry®

Ordering Information
Laser

ChannelPlate Formats

Cat. No.

iQue® 3 110V Blue-Red Configuration

2896, 384

91375

iQue® 3 HD 110V Blue-Red Configuration

28384, 1536

91381iQue® 3 110V Violet-Blue-Red Configuration

31596, 384

91377

iQue® 3 110V Violet-Yellow-Blue Configuration

31596, 38491379

Optional Products

Cat. No.

Forecyt® Enterprise Edition with 5 Floating Client Licenses

91131

Robotic Integration API License

90208

Qmax Refill Module

91309

---

Visit https://shop.sartorius.com/c/flow-cytometry
to find and order:Solutions, bottles, reservoirs, and cartridges
iQue® 3 accessories
Qmax and Qsol refill modules and buffers
Beads, plates, probes, and tubing

North America
Sartorius Corporation
300 West Morgan RoadAnn Arbor, Michigan 48108USA
Phone +1 734 769 1600
Email: orders.US07@sartorius.com

EuropeSartorius UK Ltd.
Longmead Business Centre
Blenheim Road
EpsomSurrey, KT19 9QQ
United Kingdom
Phone +44 1763 227400
Email: euorders.UK03@sartorius.com

Asia Pacific
Sartorius Japan K.K.
4th Floor, Daiwa Shinagawa North Bldg.
1-8-11, Kita-Shinagawa 1-chome
Shinagawa-Ku
Tokyo 140-0001
Japan
Phone +81 3 6478 5202
Email: orders.US07@sartorius.com

Find out more: www.sartorius.com/iqueFor questions, email: AskAScientist@sartorius.com
Specifications subject to change without notice. ©2024. All rights reserved. All names of Sartorius products are registered trademarks and the property of Sartorius AG and/or one of its affiliated companies.
iQue3-Brochure-US-2404-en-Sartorius. Status: 04 | 2024