Raw material exclusively for cosmetic manufacturing

COLLAGEN
Amphipathicity of Shark Fin
Atelocollagen MS

Compatibility of Shark Fin
Atelocollagen MS
The compatibility was checked when 0.2% Shark Fin
Atelocollagen MS and each cosmetic raw material weremixed at a 1:1 ratio and stored at room temperature.

Ten grams of 0.1% coll agensolution was added to 0.1 gof face wash foam. A vial
wa s s h a ke n 10 0 t i m es a n dleft to stand. In ﬁve minutes,
t h e o u te r a p p e a ra n ce w a sobserved.
Shark Fin
Atelocollagen

Shark Fin
Atelocollagen MS
Based on in-house data

Microscopic observation

Bar：50μm

A 0.8% collagen solution, PEG 400, and olive oil were mixed at a 1:1:1
ratio. Then, the mixture was stored for four days at 25℃.
Water

Water
solubility
CollagenSuccinoyl
atelocollagen

Raw material(ﬁnal concentration)

Immediately
after mixing

1 mo. later

0.01% sodium hyaluronate〇〇

10% glycerin〇〇

0.1% sodium alginate〇〇

0.1% xanthan gum〇

×5% ethanol〇〇

30% butylene glycol〇〇5% pentylene glycol〇〇

5% propanediol〇

Domestically produced collagen similar to baby collagen

Shark Fin Atelocollagen(soluble collagen, succinoyl atelocollagen, and myristoyl succinoyl atelocollagen)〇Based on in-house data

Relationship between pH
and solubility

Myristoyl
succinoyl
atelocollagen＊Three approaches that aid
recovery from chapping

Shark Fin
Atelocollagen
Shark Fin
Atelocollagen SS

The emulsion stability was only recognized with Shark Fin
Atelocollagen MS.

Shark Fin
Atelocollagen MS234

56789＊Oil droplets with a diameter of a few micromillimeters
were only observed with Shark Fin Atelocollagen MS.

Based on in-house data

Recommended formulation ratio: 1％Safety evaluation Human Repeat Insult Patch Test (HRIPT): Negative

Product number

Product name

AFN-221

Shark Fin Atelocollagen 1% PE

AFS-121

Shark Fin Atelocollagen SS 0.3% PE

AFS-221

Shark Fin Atelocollagen SS 1% PE

AFV-121

Shark Fin Atelocollagen SS-V 0.3% PE

AFM-521

Shark Fin Atelocollagen MS 0.8% PE

The Country of origin: Japan

Commitment tomaterial
procurement：pH permitting dissolution

INCI name ／中文名称
SOLUBLE COLLAGEN
可溶性胶原

SUCCINOYLATELOCOLLAGEN
琥珀酰缺端胶原MYRISTOYL SUCCINOYLATELOCOLLAGEN
肉豆蔻酰琥珀酰/端胶原101112

Based on in-house data

Other ingredients

Basic typePackage

Citric acid, sodium citrate,
phenoxyethanol, water
Disodium phosphate, potassium phosphate,phenoxyethanol, water

1kg

Disodium phosphate, potassium phosphate,phenoxyethanol, cellulose gum, water
Disodium phosphate, potassium phosphate,
phenoxyethanol, water

Solublecollagen
Maintains the innate
structure of skinTones the skin

SS type

Succinoyl
atelocollagen
Protects the skin
Soft and springy skin

The place of Origin: Miyagi Pref.

Domestically produced
raw materials

Eﬀective use of materials(sustainability)

We us e ﬁns of b lu e sha rk s
landed at Kesennuma, Miyagi.
Traceability has been established for the raw material toensure reassurance and safety.

We use inedible shark ﬁns. We
give consideration to the environment in the procurement ofcosmetic raw materials.

Ethical consumption
Kesennuma boasts the largest
landings of sharks in Japan. In
cooperation with local food product companies, we will support
the creation of a recycling-basedsociety and future-oriented production of cosmetics.

＊The content in this document is prepared based on documents and experimental data available at the time of preparation. However, the content
does not comprise a guarantee. ＊Please note in advance that the content description in the document is subject to change. All intellectual property
belongs to KOKEN CO., LTD., such as copyrights and design rights for the given data and related documents. All rights reserved. For safe use of thematerials, refer to the MSDS of each of our products prior to use. For written and verbal expressions about products for general consumers, follow
the related laws and regulations in each country.

Contact

KOKEN CO., LTD.
1-4-14 Koraku, Bunkyo-ku, Tokyo 112-0004, Japan
Telephone: +81-3-3868-0560

Facsimile: +81-3-3816-3570

https://koken-cosme.com/Ⓒ2021 KOKEN CO., LTD. C-1-805-17-1-01-00

MS type

Myristoyl
succinoyl
atelocollagen
Long-term eﬀect throughamphipathicity
Softens the skin

Domestically produced collagen similar to baby collagen

Moisturizing eﬀects on the skin and results of sensory evaluation (human testing)

Shark Fin Atelocollagen

Each test article of shark ﬁn atelocollagen showed moisturizing eﬀects. In particular, Shark Fin Atelocollagen MS was found to continuously retain a higher amount of moisture in the horny layer (the long-term eﬀect). Select Shark Fin Atelocollagen for toning,
Shark Fin Atelocollagen SS for softness and springiness, or Shark Fin Atelocollagen MS for softness.

Moisture content (test sample:a general emulsion formulation containing 1% of each raw material) （n=2）

SS typeMS typeSolublecollagenSuccinoyl
atelocollagen

Myristoyl
succinoyl
atelocollagen

Atelocollagen has the original triple
helix structure of collagen while thetelopeptides, which are responsible
for allergic reactions, are removed.

Basic structure of atelocollagen
NH 2COOH

The collagen features increased solubility by bonding moisturizing ingredients. It protects the skin from irritants
such as surfactants.

The novel collagen is produced by combining lipophilic ingredients and moisturizing
ingredients with collagen using our unique
technology. Recommended for cleansing
items because it maintains a moisturizing
eﬀect even after being washed away.

Water-retaining ingredient

Water-retaining ingredient

NHCONH 2

CONH 2COOH

NH 2NHNH

CONH 2COOH

NH

Water-retaining ingredient

Lipophilicingredient‑CO‑(CH 2) 12 ‑CH 3‑CO‑CH 2‑CH 2‑COOH Succinoyl group

Myristoyl group

Contains the rare ingredient elastoidin
Elastoidin is a type of rare collagen that can be only found in shark ﬁns. It has a homotrimer structure with functions similar
to Type III collagen. Shark ﬁn atelocollagen contains collagen ingredients, about 50% of which is elastoidin.
General atelocollagen

Shark Fin AtelocollagenContains a single type ofcollagenContains two typesof collagenType Ⅰ collagenType Ⅰ collagen

α1α1α2：■ Type Ⅲ collagen
It is generally called baby collagenbecause it is generated in a large
quantity during the fetal period.
In a baby's skin,

They form the base for ﬁnely textured, elastic skin.

about 50%

Type Ⅰ collagenType Ⅰ collagen

300

Type Ⅲ collagen

250
ug/g

Shark Fin Atelocollagen SS

0.95
0.90

Shark Fin Atelocollagen MS
Before application

20 minutes later

60 minutes later

Relative changes in moisture content in the horny layer
15 minutes after cleansing (concentration of collagen: 0.01%)Approximately 16 times

15.0

Elderly51〜High

Approximately 10 times

10.0■ The eﬀect of alleviating stimuli on activators
All types of shark ﬁn atelocollagen were shown to protect
the skin from external stimuli.
Testing methods: SDS, a stimulant, was applied on the artiﬁcial skin thatwas cultured after the application of each sample. The cultivation continued after cleansing. The living cell rate was determined using an MTT
assay.
Comparison of living cell rates (concentration of collagen: 0.05%)
12010080604020

5.00.0SoftnessHydrolyzed
collagenShark Fin
Atelocollagen

Shark Fin
Shark Fin
Atelocollagen SS Atelocollagen MSLow0

Water

Hydrolyzed
collagenShark Fin
Shark Fin
Shark Fin
Atelocollagen Atelocollagen SS Atelocollagen MS
Based on in-house data

Skin barrier and skin recovering effects (human testing)Testing methods: The chapped skin was prepared, each sample wascontinuously applied at least once a day, then TEWL was measured onday 7.

（n=7）

Testing methods: The chapped skin was prepared and Shark Fin Atelocollagen MS was continuously applied at least once a day. Three days
later, the skin was visually checked and compared on VISIOSCAN images.
Visually checked on VISIOSCAN images(concentration of collagen: 0.01%)

0.53

Water

Shark Fin Atelocollagen MS

Chapping was foundSkin recovery was recognized

0.51
0.50

Type Ⅲ collagen

0.46

α1
α1
α1

Adult
19〜50

Softness and springiness

Based on in-house data

Testing methods: Each sample was applied on the inner part of the forearm and washed away with water. Then, the forearm was wiped dry.
After 15 minutes, moisture content in the horny layer was measured tocheck changes. Relative values were calculated while moisture content in
the horny layer with hydrolyzed collagen was set to 1.

20.0

Shark FinAtelocollagen MS

Skin protecting eﬀects (cell testing)■ Moisturizing effects after cleansing
Each shark fin atelocollagen presented moisturizing effects
about 10 to 16 times higher than hydrolyzed collagen andsuggested high adhesiveness to the skin. In particular,
Shark Fin Atelocollagen MS is expected to maintain moisture longer because it is difficult to wash away completely.

Chappedskin

0.49
0.48
0.47

100

Adolescent〜18Toning

180 minutes later

Adhesiveness to the skin andmoisturizing effects (human testing)Comprises two kinds ofprotein (heterotrimer)

150

Fetus

Shark FinAtelocollagen SS
Shark Fin Atelocollagen

0.52

α1α1α220050

Shark Fin Atelocollagen
Shark Fin Atelocollagen SS
Shark Fin Atelocollagen MS

Based on in-house data

Relative value after seven days of continued application withchapped skin deﬁned as 1 (concentration of collagen: 0.01%)

350

0Shark Fin Atelocollagen

1.00

Improvement

Type Ⅰ collagen andType Ⅲ collagen exist
in a 50:50 ratio.

1.10
1.05（n=17）

Each shark ﬁn atelocollagen will suppress transepidermal water loss when applied to the skin. It is expected to improve theskin barrier function. The skin recovered more obviously from chapping after application of Shark Fin Atelocollagen MS.

α1（E）
α1（E）
α1（E）

about 50%

1.15

Sensory
evaluation

Based on in-house data

Collagen similar to Type Ⅲ collagen (Elastoidin)

α1
α1
α2

1.20

Moisture content in the horny layer(relative value)

Basic type

Moisture content (relative value)atelocollagen for diﬀerent uses in cosmetic products. Choose the appropriate one according to its type.

Relative value whenatelocollagen is deﬁned as 1

is natural marine atelocollagen that contains rare homotrimer collagen (elastoidin). We oﬀer three types ofTesting methods: After washing the face with soap, 50 μL of each sample was applied to the entire face. Then, the moisture content in the horny layer wasdetermined. Chronological relative changes were calculated while the moisture content in the horny layer before use was set to 1.

Living cell rate (%)

Atelocollagen is a hydrophilic protein with a high moisture-retaining property. Shark-ﬁn-derived atelocollagen

Comprises a single kind ofprotein (homotrimer)

Measured values cited from African Journal of Biotechnology Vol. 10(13), pp. 2524-2529, 28 March 2011

0.45
0.44
0.43

Hydrolyzed
collagenShark Fin
Atelocollagen

Shark Fin
Shark Fin
Atelocollagen SS Atelocollagen MS
Based on in-house data

Three days
afterapplication

Based on in-house data