Comprehensive
Media Solutions forYour Protein-BasedTherapies

Sartorius as YourMedia Solutions
Partner
Chinese hamster ovary (CHO) cells
are ideal for therapeutic protein
production in biopharmaceuticalresearch and development.
Our comprehensive offering ofseveral CHO cell culture media
and companion feeds, combinedwith our advanced cell line
development and supporting
media services, enables simplified
clone selection and optimized
titer production.
Backed by expert support and a
global manufacturing network,
we provide what’s vital to your cells
and your success — from screening
to commercial production.

Request a Sample
www.sartorius.com/en/pr/
request-cell-culture-media-sample

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4Cell® CHO Platform | CHO Media and Feeds | Media Services

4Cell® CHO Platform
The 4Cell® CHO Platform bundles services and media in a GMP manufacturing-ready solution for drug developers looking
for stable and ready-to-scale clones.
Cell Line Development Service

Protein Characterization Service

The platform comprises an advanced CLD service using
Sartorius' high-throughput CellCelector™ technology forSingle Cell Cloning that can deliver high-performing clones
in 9 weeks with demonstrated performance and titers up to10 g/L in the optimized 4Cell® SmartCHO media.

Sartorius' protein characterization services are scaled toeach phase of drug development. These services include
the development, qualification, and validation of productcharacterization assays, such as cell-based potency assays,
binding assays, and physicochemical tests.

Cell Culture Media

Cell Bank Manufacturing And Biosafety Testing

The 4Cell® SmartCHO Media was specially developed for ourcell line development service and supports the productionof RCB and MCB. It is a high-quality, high-performance cell
culture formulation that is chemically defined and free ofanimal components to maximize product titer in our CHO
cell line. 4Cell® SmartCHOpe Media, our perfusion medium,is compatible with our CLD service and supports clone selection in a perfusion mimic protocol.

The Sartorius cell banking platform is designed as a closed,sterile, single-use system with automated filling into vials
to generate large and consistent banks. Plus, Sartorius has
extensive experience in characterizing cell banks used in theproduction of protein-based therapies.

Services

DNA to Cell Banks
Predictable performance and accelerated drug development with our integrated CHOplatformCell Line DevelopmentProtein Characterization

Cell Banking

Media4Cell® SmartCHO Media

4Biosafety testing4Cell® CHO Platform | CHO Media and Feeds | Media Services

CHO Media and Feeds
Sartorius offers a wide range of media and feeds to provide drug developers with the best formulation for their stable clones,
enabling a successful transition to the clinic with high-performance cell growth and productivity.
Chemically defined, animal ingredient-free, protein-free, serum-free, hydrolysate-free, and GMP-compliant media to support
the expansion of the most common CHO cell lines in all applications.
CHO Transfection MediaProductFormat

Features

CHO TF

Bottle (1 L), powder (10 L, 50 L)

Ideal medium for working with transient CHO cells

CHO Batch and Fed-Batch Media
Product

FormatFeatures

4Cell® SmartCHO SAM

Bottle (1 L), bag (1 L, 5 L, 20 L),
powder (10 L, 25 L, 250 L)

Allows the cultivation or media adaptation of CHO cells

4Cell® SmartCHO PM

Bottle (1 L), bag (1 L, 5 L, 20 L),
powder (9 L, 20 L, 200 L)

Non-selective production media for batch or fed-batch culture. It can be used for effective
cultivation of any CHO cell lines, and helps produce high yield of the active protein

4Cell® SmartCHO FMA

Bottle (1 L), bag (1 L, 5 L, 20 L),
powder (3 L, 30 L)

Ideal companion feeds designed to be used together with
4Cell® SmartCHO Production Media

4Cell® SmartCHO FMB

Bottle (125 mL, 500 mL),bag (1 L, 5 L), powder (4 L, 45 L)

Ideal companion feeds designed to be used together with
4Cell® SmartCHO Production MediaCHOleanBottle (1 L),
powder (10 L, 50 L, 100 L)

Lean and robust growth media for a variety of applications, lean formulation with minimalcomponents ideal for media optimization activities

TC-42

Bottle (1 L), powder (10 L, 50 L)

Workhorse formulation especially designed for CHO-DHFR cell lines

TCX10D

Bottle (1 L), powder (10 L, 50 L,100 L, 200 L, 500 L)

For long-term high-performance growth with CHO-GS cell lines

TCX6D

Bottle (1 L), powder (10 L, 50 L,100L, 200 L, 500 L)

One high-performing media for any CHO clones with growth factor LONG® R3IGF-I

TCX7D Feed
Supplement

Bottle (1 L), powder (2 L, 5 L, 10L, 25 L, 50 L, 100 L)

With 60 g/L D-glucose, especially for CHO GS cell lines

Basic Feed

Bottle (1 L), bag (10 L, 20 L,50 L), powder (5 L, 10 L, 50 L)

With 20 g/L D-glucose, also suitable for hybridoma

54Cell® CHO Platform | CHO Media and Feeds | Media Services

CHO Perfusion MediaProductFormatFeatures

4Cell® SmartCHO SAM

Powder (10 L, 25 L, 250 L)

Allows the cultivation or media adaptation of CHO cells

4Cell® SmartCHO PM

Powder (20 L, 50 L, 200 L,900 L)

Non-selective production media for perfusion culture

4Cell® SmartCHO FMA

Powder (3 L, 30 L, 120 L)

Ideal companion feeds designed to be used together with 4Cell® SmartCHOpe
Production Media4Cell® SmartCHO FMB

Powder (2 L, 4.5 L, 45 L, 180L)

Ideal companion feeds designed to be used together with 4Cell® SmartCHOpe
Production Media64Cell® CHO Platform | CHO Media and Feeds | Media Services

Media Optimization
Services

Custom Media Development
And Manufacturing Services

Supporting customers in optimizing their media andprocesses to prepare for scale-up, decreasing theirtime-to-market while maintaining high titer and productquality

Targeted, rapid, and efficient services for the developmentof chemically defined, animal component-free, custom
cell culture media and feed, available as liquid or powder
formulations.

----Cell Line Adaptation Service
Media and Process Optimization ServiceBenchmarking and Material Testing
Tech Batches and Feasibility Studies
Spent Media Analysis

7GermanySartorius Stedim Biotech GmbH
August-Spindler-Strasse 1137079 Goettingen
Phone +49 551 308 0USA
Sartorius Stedim North America Inc.
565 Johnson Avenue
Bohemia, NY 11716
Toll-Free +1 800 368 7178

For further information, visit	
www.sartorius.com

Specifications subject to change without notice.
©2023 Sartorius Stedim Biotech GmbH, August-Spindler-Strasse 11, 37079 Goettingen, Germany
Status: 11 | 2023