Service Datasheet

Tailor-MadePlasmid Service
Engineering and Production
Services for Plasmids From
Discovery to GMP-GradeService Information
Our unique and innovative DNA assembly technology,e-Zyvec®, utilizes linear DNA bricks to design and assemble
tailor-made plasmids. This allows for fully flexible and accurate plasmid design, enabling the generation of any plasmid,from simple to highly sophisticated, without restrictions.
Using our online plasmid platform, you can design your­plasmid, share it with your team, and consult with our experts
who can help identify your needs from the start of your­project. You will receive ready-to-use, tailor-made plasmids
that meet your project's expectations. Additionally, the fulllength of your plasmid will be validated by next-generation
sequencing as a quality control measure.

---Features and Benefits
Innovative Technology (e-Zyvec®): Seamless DNA­assembly to produce even highly complex plasmids
De-Risking: Access to dedicated plasmid experts
and an ­online design platform
Reliable Plasmid Sequence: Full-length plasmid­sequencing included
Time-Saving: Ready-to-use plasmids within three weeksof orderingIntroduction---Relevant Applications

Tailor-Made Plasmid Service Workflow
1. Access: Log in to our online design platform.

Fundamental research
Cell and gene therapy
mRNA and DNA therapeutics
Protein and antibody engineering

2. Initiate: Start a new plasmid project.
3. Select: Choose to design a plasmid from scratch or use
a suggested template.

Quality Grade Provided
Our tailor-made plasmid service provides plasmid­amplification up to several milligrams at discovery grade(miniprep and whole-plasmid sequencing are included
as quality control).
Higher quantity and | or quality (up to GMP-grade) can beprovided through our manufacturing services

4. Design: Start to assemble a plasmid from scratch or use thesuggested template. Add and move any sequence from ourdatabase. ­Everything is flexible, nothing is restricted.
5. Review: Submit your design for a complimentary expert
plasmid evaluation.
6. Confirm: Approve the final design and specify the ­required
plasmid quantity.
7. Track: Stay updated on the production progress of yourplasmids through our online platform.
8. Quality Control: Your plasmid is validated thanks to­different quality control measures, including whole-plasmid
sequencing.
9. Move Science Forward: Finalize your project with ourready-to-use plasmids.

Codonoptimization
Any kind of plasmid(e.g., pTransgene,Multicistronic)

××
××
×

Long sequenceof interest

Test any promoter,
sequence of interest, etc., in parallel

2

Different tags
at different places×
Site-directed­mutagenesis

And more …
No catalog, no limits.

Ordering Information

End Product Information

To proceed with the order, register at
www.polyplus-sartorius.com/register, create a project, andstart designing your ­plasmid with our easy-to-use platform.

We deliver discovery grade plasmids as circular, double-­stranded DNA molecules, in a screw-cap plastic tube,­re-suspended in 1 × AE buffer (miniprep) or 1 × TE buffer(midi-, maxi-, giga- preparation.), at room temperature.
Please note that we do not provide bacterial stock.

After designing your plasmid on our online platform, you canget in touch with our plasmid scientist to review your plasmids
and get advice! Once you validate the design, our experts
and our proprietary software will engineer your plasmid
to maximize your chances of success (99% success rate).
For tailor-made plasmid projects, desired sequences can­typically be generated using DNA synthesis (outsourced).
However, some projects may require you to send us DNA
material (plasmid templates or purified genomic DNA)
that we can use to overcome technical complexity, ensure­technical continuity, or reduce cost. In this case, please
follow these instructions:Use a 1.5 mL plastic tube, hermetically closed(screw cap or parafilm)
Label the tube clearly
Minimal volume: 15 µLMinimal concentration: 100 ng/µL
Room temperature, please avoid dry ice----

For research-, high quality- and GMP-grade, please refer
to our plasmid manufacturing service.

----

Quality Controls
Limited to basic quality and identity tests:
DNA quantification
A260/280 and A260/230 measurements
Restriction digest profile (gel or capillary electrophoresis)
Whole-plasmid sequencing, including mycoplasma andgenomic DNA detection (by next-generation sequencing)
Sanger sequencing (if necessary)
Quality control results will be documented in the Product
Delivery Form. Endotoxin levels are not measured for­discovery grade plasmids.

--Storage
Upon receipt, briefly spin down the tubes containing yourengineered plasmids (bench centrifuge) and store them at:
4 °C for short storage (<1 month)−20 °C for long storage
We strongly recommend users aliquot discovery grade­plasmids for long storage and to avoid freeze-thaw cycles.
Duration of storage is given here as an indication. Always­verify the integrity of plasmids stored for extended periods
by gel electrophoresis (denaturation) or sequencing­(sequence integrity).
Intellectual Property
Unless otherwise stated in the specifications of your project,
every engineered plasmid provided on a service-based­contract for research applications is ­IP-free from Sartorius,
as stipulated in our T&C. Other applications may require a­licensing agreement with Sartorius or tiers entity.

3Germany
Sartorius Stedim Biotech GmbH
August-Spindler-Strasse 1137079 Goettingen
Phone +49 551 308 0USASartorius Stedim North America Inc.
565 Johnson Avenue
Bohemia, NY 11716
Toll-Free +1 800 368 7178

F or more information, visit
sartorius.com/transfection-reagents-plasmidsSpecifications subject to change without notice.
©2024 Sartorius Stedim Biotech GmbH, August-Spindler-Strasse 11, 37079 Goettingen, Germany
Status: 03 | 2024

FrancePolyplus®—Now part of Sartorius
75 Rue Marguerite Perey
67400 Illkirch
Phone +33 390 406 180