Accelerate COVID -19 researchusing the highly sensitive
RNAscope™ In Situ
Hybridization technology
The RNAscope technology:
PERMEABLIZE
cells or tissue
TISSUECELLS

HYBRIDIZEto target RNA
RNAscope
Double Z Probe
Preamplifier
Binding SiteAMPLIFY
signalVISUALIZEwith morphology

QUANTIFY
single-cellexpression

Lable Probe

x20

AmplifierPre-amplifier

RNA Target

RNAscope
Double ZProbe

The RNAscope technology is a novel in situ hybridization method that is designed to detect target RNA in intact cells
by using proprietary probes that simultaneously amplify target signal sequences while suppressing background noisefrom non-specific hybridization. This technology obviates the need for the costly and time-consuming developmentof specific antibodies for newly identified gene targets or pathogens such as the COVID -19 SARS-CoV-2 virus.
Additionally, the RNAscope technology can be combined with immunohistochemistry (IHC) on the same slide fordetection of RNA and protein simultaneously. With an ever-growing catalog of probes for over 25,000 targets in
300+ species and the option of made-to-order target probes, the RNAscope technology has become an essential
technology for biomarker assessment, featured in over 2,700 publications across multiple different research areas
including infectious disease, inflammation, neurobiology, cancer biology, and immuno-oncology.

Publications highlighting RNAscope in infectious disease research:
We have over 400 publications using the RNAscope technology for viral detection. Listed below are a few key publications on positive single-stranded RNA viruses.
1. Haagmans, B.L. et al., An orthopoxvirus-based
vaccine reduces virus excretion after MERS-CoV
infection in dromedary camels. Science, 2016.
351(6268): p. 77-81.

Detection of positive single-stranded RNA virus

2. Cha, R.H.Y., et al., A Case Report of a Middle EastRespiratory Syndrome Survivor with Kidney 		Biopsy Results. J Korean Med Sci, 2016.
31(4): p. 635-640.
3. Haagmans, B.L. et al., Asymptomatic Middle 		East respiratory syndrome coronavirus infection
in rabbits. J Virol, 2015. 89(11): p. 6131-6135.
4. Vergara-Alert, J.v.d.B., et al., Livestock Susceptibility to Infection with Middle East Respiratory
Syndrome Coronavirus. Emerg Infect Dis, 2017.
23(2): p. 232 - 240.
5. Bhatnagar, J.R., et al., Zika Virus RNA Replication and Persistence in Brain and Placental
Tissue. Emerg Infect Dis, 2017. 23(3): p. 405-415.

V-ZIKA-pp|Uninfected Brain

V-ZIKA-pp|

Infected Brain

Detection of ZIKA virus strain PRVABC59 in mouse tissues: The RNAscopeprobe detected positive staining for ZIKA viral RNA in the infected tissues
but not in the uninfected tissues demonstrating the specificity of theRNAscope probes.

How can the RNAscope technology support COVID -19 research?
Applying the RNAscope technology to your COVID -19 research can provide new insights intothe pathogenesis of SARS-CoV-2 and aid in vaccine and drug development efforts by helping
researchers answer the following questions:
1. Which tissue(s) does SARS-CoV-2 infect?
RNAscope assays allow for direct visualization of the virus and estimate the viral load in cellswith morphological context. The V-nCoV2019 -S probe detects the SARS-CoV-2 spike proteinmRNA and does not detect other coronaviruses or host mRNA.
2. Which cell type(s) does SARS-CoV-2 infect?
Using the RNAscope multiplex assays, V-nCoV2019-S probe can be combined with probes for the cellular receptor
ACE2 and proteases such as TMPRSS2, Cathepsin B and Cathepsin L, all of which facilitate viral entry into the host 		cells, for the visualization of infected cells. The single-molecule sensitivity of RNAscope is particularly well-suited for 		
detecting low levels of ACE2 expression and identifying virus-targeted cell types.1
3. What inflammatory response does the virus induce in infected patients?
Patients with severe COVID -19 infections suffer from acute respiratory distress syndrome (ARDS) 2 which is induced 		by the release of cytokines such as IL-6, IL-1β, IL-10 and TNFα among others.3 RNAscope assays can identify the 		cells secreting these inflammatory cytokines.
4. In which tissues/cells does SARS-CoV-2 replicate?
The RNAscope assay can detect viral replication in tissues by using the V-nCoV2019 -S sense probe targeting the 		antisense RNA strand produced during viral replication.
Listed below are probes and reagent kits for manual RNAscope assays.
Corresponding probes and reagent kits for automated assays are also available.
RNAscope tools available for COVID-19 researchCAT. NO. PROBE

848561845701848151470341602051310371310361310421

RNAscope® Probe - V-nCoV2019-S
RNAscope® Probe - V-nCoV2019-S-sense
RNAscope® Probe - Hs-ACE2
RNAscope® Probe – Hs-TMPRSS2
RNAscope® Probe – Hs-IL10
RNAscope® Probe – Hs-IL6
RNAscope® Probe – Hs-IL1B
RNAscope® Probe – Hs-TNFATARGET

Viral spike protein
Spike protein sense strand
Host cell receptor
Serine protease
Cytokine
Cytokine
Cytokine
Cytokine

CAT. NO. ASSAY
322300

RNAscope® 2.5 HD Reagent Kit – BROWN

322350

RNAscope® 2.5 HD Reagent Kit – RED322430

RNAscope® 2.5 HD Duplex Reagent Kit

323100

RNAscope® Multiplex Fluorescent Reagent Kit v2

Order probes: https://bit.ly/rnascope-probes
Learn more about RNAscope application for viral parthenogenesis research:acdbio.com/science/applications/research-areas/infectious-diseases
References:
1. Hoffmann, M.; Kleine-Weber, H.; Schroeder, S.; et al. S. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor.
Cell 2020, DOI: 10.1016/ j.cell.2020.02.052.
2. Z Xu, L Shi, Y Wang, et al. Pathological findings of COVID-19 associated with acute respiratory distress syndrome. Lancet Respir Med 2020 ,DOI: 10.1016/S2213-2600(20)30076-X.
3. Park WY, Goodman RB, Steinberg KP, et al. Cytokine balance in the lungs of patients with acute respiratory distress syndrome. Am J Respir Crit Care Med 2001,
DOI: 10.1164/ajrccm.164.10.2104013.

To request a quote, contact: acd_sales@bio-techne.com

7707 Gateway Boulevard, Newark, CA 94560 1.510.576.8800 (Main) | 1.877.576.3636 (Toll Free)For Research Use Only. Not for diagnostic use. RNAscope and BaseScope are trademarks of Advanced Cell Diagnostics, Inc. in the United States orother countries. All rights reserved. 2020 Advanced Cell Diagnostics, Inc. Doc #: MK 51-145/REV A/Effective Date 03/31/2020