Microbial MOS VS HYPO Comparison Table
A Compilation of Data Showing Mixed Oxidant Solution Superiority in Microbial Inactivation
Compared to Hypochlorite (Bleach)
MIOX mixed oxidants have been proven in numerous laboratory studies to achieve more rapid and thoroughinactivation of a wider range of microbial contaminants, including species highly resistant to chlorination, thanconventional chlorine. The table below illustrates this point. Further, The MSR MIOX Purifier, the hand-heldversion of MIOX technology, passes the Environmental Protection Agency’s (EPA’s) Guide Standard andProtocol for Microbiological Purifiers, achieving more than the required inactivation level for viruses (4 log),
bacteria (6 log), and protozoan oocysts (3 log) in both clean and dirty waters

Microorganism

MIOX Bleach
DoseDose(mg/L) (mg/L)

Contact ContactTimeTimeMIOX
Bleach(min.)(min.)

Inactivation Differentiating
Reference(log)Parameter

BACTERIA
Vibrio cholerae
Escherichia coli
Pseudomonas
aeruginosa
Legionella
pneumophilia
Staphylococcus
aureusListeria
monocytogenes

Bacillus
sterothermophilus
Clostridium
perfringens spore
Bacillus globigii
sporesBacillus anthracis(Sterne) spores

22

1.8

4.04 log

Time223.8

5.04 log

Time22101022224422442222.5Efficacy

2>4.8 MIOX
2.2 Bleach1010>5.0 MIOX
4.7 Bleach
1.6 MIOX
60
60
0.8 Bleach
3.7 MIOX
6060
2.3 Bleach
2.0 MIOX
60
60
0.8 Bleach
3.7 MIOX
6060
1.2 Bleach
BACTERIAL SPORES
3030>5 MIOX
2.5 Bleach13182 log

2.515Efficacy

15

3.6 MIOX
2.4 Bleach

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Efficacy

Efficacy

EfficacyTime

Venczel etal, 2004
Venczel etal, 2004
Barton,
1996
Barton,1996

Russell,
2001*

Barton,1996
Venczel etal, 2004
Bajszar,2008 andCDC,
2009†

Microorganism

MIOX Bleach
Dose
Dose
(mg/L) (mg/L)

Contact ContactTime
Time
MIOX
Bleach(min.)(min.)

Inactivation Differentiating
Reference(log)Parameter

VIRUS
MS2 Coliphage
Vaccinia(Smallpox
Surrogate)
Poliovirus Vaccine
Strain 1
Rotavirus SA-112270168

4 log

Time

5~7020104 log MIOX
3 log Bleach>4NA30

NA>4NA30

NA>5.5 log
MIOX>5.5 log
MIOX

Concentratio
nTime
Efficacy
NAVenczel etal, 2004
Wright et al,
2001
Groupé, V. etal, 1955
Biovir, 2002

NABiovir, 2002

PROTOZOAN OOCYSTS
Giardia lamblia
Cryptosporidium
parvum oocyst

Cryptosporidium
parvum oocyst>4NA30NA>4 log MIOX

NA

Biovir, 2002

55240

1440

Time andEfficacy

Venczel etal, 1997

2525240240

3, MIOX
None,
Bleach>1, MIOX
0.25 Bleach

Efficacy,qRT-PCRand tissue
cultureinfectivity

Bajszár &Dekonenko
2010*These data were collected using the Impedance Detection Method (IDT) at pHs ranging from 6.5-9.0.
† Researchers at the Centers for Disease Control obtained similar results using a different strain of spore.

5601 BALLOON FIESTA PARKWAY / ALBUQUERQUE, NEW MEXICO 87113 USA
505.343.0090 / 888.646.9420 / FAX 505.343.0093 / INFO@MIOX.COM / WWW.MIOX.COM

References:Bajszár, G. and Dekonenko, A. 2010 “Stress-induced Hsp70 Gene Expression and Inactivation ofCryptosporidium parvum oocysts by Chlorine-Based Oxidants” Applied and Environmental Microbiology
76, 6: 1732-1739.
Bajszár, G. “Spore Inactivation Tests for Disinfectant Comparison and Biocidal Efficacy Assessment”,May 30, 2008.
Barton, L.L., “Disinfection of Simulated Cooling Tower Water” Department of Biology, University of
New Mexico, Albuquerque, NM, March, 4, 1996.
BioVir Laboratories US EPA Guide Standard and Protocol for Testing Microbiological Purifiers, under
the direction of Dr. Rick Danielson, “Product Microbial Disinfection Study fro Los Alamos TechnicalAssociates Inc., MIOX™ Water Disinfection Pen” BioVir Laboratories, October 15, 2002.
Dekonenko, A. and Bajszár, G. 2009. Disinfection of feline calicivirus (a human norovirus surrogate) onsurfaces with chlorine-based mixed oxidant solution. Internal Report (manuscript in progress).
Groupé, V., Engle, C.G., Gaffney, P.E. and Manker, R.A. 1955, “Virucidal Activity of Representative
Antiinfective Agents Against Influenza A and Vaccinia Viruses,” Applied and Environmental
Microbiology; 1955 3: 333-336
Russell, S.M., “A Comparison of MIOX versus Bleach for Eliminating Pathogenic Bacteria Associatedwith Poultry Products”, Department of Poultry Science, University of Georgia, Athens, GA June 1, 2001.
Rose, L., “Evaluation of the Efficacy of Mixed Oxidants on Bacillus anthracis (Sterne) Spores Associatedwith a Model Drinking Water Biofilm”. Center for Disease Control Reference #: NCPDCID-RC08494700, Final Report May 12, 2009.
Venczel, L.V., Likirdopulos, C.A, Robinson, C.E., and Sobsey, M.D., 2004, “Inactivation of EntericMicrobes in Water by Electro-chemical Oxidant from Brine (NaCl) and Free Chlorine,” Water Scienceand Technology; 50, 1; 141-146.
Venczel, L.V., Arrowood, M., Hurd, M., and Sobsey, M.D., 1997 “Inactivation of Cryptosporidium
parvum Oocysts and Clostridium perfringens Spores by a Mixed-Oxidant Disinfectant and by Free
Chlorine,” Applied and Environmental Microbiology; 63, 4; 1598-1601.
Wright, J.D., T.J. Dvorak, and B.G. Harper, 2001, “Interim Report: MIOX Disinfection Pen and the
MesoSystems MesoStill™ Agents of Biological Origin (ABO) Disinfection Test”, Life Sciences
Division, West Desert Test Center, U.S. Army proving Ground, Dugway, UT, Test Project No. 8-ES-225MOX-001, WDTC Document No. WDTC-IR-01-047, May 2001.

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505.343.0090 / 888.646.9420 / FAX 505.343.0093 / INFO@MIOX.COM / WWW.MIOX.COM