MIS03-01

Room:104

Time:May 27 09:00-09:45

Volcanic islands as model systems to quantify pedogenic thresholds and determine theirimpact on Polynesian land-use
CHADWICK, Oliver1∗
1

University of California, USA

Soils and weathering profiles are complex emergent features at the surface of terrestrial Earth. They form a boundary layer
hosting the chemical and physical interaction of biology and hydrology with rock. Soil development derives from the dissipationof chemical energy through reaction with rock minerals; few of the original reactants survive, but they are replaced by secondary
products unique to the weathering environment. Soil processes filter and transform gasses and liquids passing through them,which in turn leads to chemical and mineralogical evolution of the fabric of soil itself. Soil chemical reactions are controlled
by a number of feedbacks that buffer the system from rapid changes in external inputs, however there are limits beyond which
the chemical system rapidly shifts into a new chemical domain governed by different buffer reactions. Globally soil distribution
patterns are underlain by specific soil process domains that are separated by pedogenic thresholds at points of domain failure.
Considering how soil process domains and thresholds determine the global distribution of properties, which control everythingfrom agricultural productivity to carbon sequestration is a primary research focus for modern biogeochemistry. Soils are complex
systems, which makes it difficult to develop clear understanding of how specific driving factors control soil process domains. It
is common therefore to develop model systems that allow us to tackle specific questions with fewer complications. Here I discuss
the use of volcanic islands in the Pacific Ocean as a model system to study how pedogenic thresholds control phosphorus andcalcium availability to plants. I then combine that knowledge with archeological information to understand how the geographic
constraints imposed by these thresholds determined Polynesian land use and agricultural productivity. As Polynesians radiatedacross the Pacific they encountered islands that presented many different local environments ranging from reef protected lagoons
and minimal high island terrain to high volcanic islands with still active volcanoes. They brought with them an agricultural starter
kit, but from there on they needed to adapt to their new surroundings. They practiced two major types of intensive agriculture:
non-irrigated dryland and flooded-field irrigated farming. Typically young islands had soils with rich nutrient stores, whereas
older islands had depleted soils that were not productive. By contrast, young islands had few large valleys to support irrigatedagriculture, whereas the older ones had broad valleys allowing development of highly productive irrigation systems. Thus cultivators in rainfed systems brought their crops to areas where near-surface rocks were still weathering and supplying nutrients suchas calcium and phosphorus, whereas in irrigated systems flowing water brought the products of weathering to crops. The contrast
had profound implications for the development of culture across Polynesia. Behind these anthropological observations lies anequally fascinating story about how dramatically different soil properties are produced by non-linear responses to environmental
forcing. In this talk I will explore the pedology of volcanic islands and relate it to pre-industrial human land-use decisions.
Keywords: Soil Chemistry, Biogeochemistry, Andisols, Soils

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MIS03-02

Room:104

Time:May 27 10:00-10:30

Can soil properties alone predict ecosystem processes?
KITAYAMA, Kanehiro1∗
1

Forest Ecology, Kyoto University

Soils are a reservoir of nutrients, which supplies plants with essential elements. Soil chemical properties can change spatiallyand temporary in relation to parent materials, climate and pedogenesis as a function of time. In places, the concentrations ofessential elements in soils are extremely impoverished, which can eventually limit the net primary productivity of terrestrial
ecosystems. Accounts by Elser et al. (2007), Vitousek et al. (2010) and many others indicate that available P is depleted indeeply weathered soils in the tropics due to a long process of geochemical occlusion and losses without substrate rejuvenationand that P limits the productivity of many tropical rain forests.
Colleagues and I have been extensively studying soil P fractions and productivity in Bornean tropical rain forests. Indeed,
the concentrations of soil total P or labile P fraction are generally impoverished in comparison to temperate ecosystems but
can still vary greatly reflecting parent materials or the status of pedogenesis. For instance, the concentrations of soil total P inseven tropical rain forests below 700 m asl in north Borneo are generally low, but range from 66 to 512 (µg/g). That of Bray-1
extractable P ranges from 0.2 to 4.2 (µg/g). Yet, above-ground net primary productivities of these forests are remarkably highand converge to a narrow range. Therefore, soil chemical properties do not correlate with productivity in plausibly P-limited
tropical rain forests. Why soil P fractions cannot explain productivity?
We ecologists consider net primary productivity as a fundamental ecosystem process, which is expressed as the rate of net
carbon fixation in an ecosystem context. Trees invest P for carbon fixation and the efficiency of the carbon fixation is expressedby the ratio of C flux to P flux, i.e. P-use efficiency in productivity. In these forests, P-use efficiencies greatly vary by 5-foldfrom 800 to 4000 (gC/gP) indicating that trees can adjust to the magnitude of P deficiency to maintain productivity. This is the
major reason why soil P fractions cannot explain productivity.
Biological mechanisms to explain the enhancement of P-use efficiencies are two fold, one is the efficiency of photosynthetic
C fixation per unit P in leaves and the other is the residence time of P in tree bodies. Colleagues and I investigated relative
importance of these two mechanisms and found that the variation of residence time of P can much better explain the variation ofP-use efficiency. Tropical trees increase the residence time of P in their bodies by increasing leaf life span and also by increasing
P resorption from senescing leaves when facing to increasing P deficiency. We consider these are the two important plant traits
which plants have acquired as adaptation. Our studies imply that understanding adaptive mechanisms as well as soil chemical
properties is essential to understand ecosystem processes.

Keywords: Adaptation, Biogeochemistry, Net primary productivity, Nutrient-use efficiency, Soil P fractions, Tropical rain forest

1/1

MIS03-03

Room:104

Time:May 27 10:30-10:45

Linkage of root physiology and morphology as an adaptation to soil phosphorus impoverishment in tropical montane forests
USHIO, Masayuki1∗ ; FUJIKI, Yasuto2 ; HIDAKA, Amane3 ; KITAYAMA, Kanehiro4
1

Ryukoku Univ., 2 MURC, 3 Hokkaido Univ., 4 Grad. Sch. Agric., Kyoto Univ.

Tropical forests in Borneo maintain a high level of productivity/biomass even under phosphorus (P)-limited conditions. The
P-acquisition properties of roots may be an important factor that contributes to forest productivity, but they have not been wellevaluated compared with aboveground properties of plants. In the present study, we analyzed root acid phosphatase activity andmorphological properties (surface area, diameter and tissue density of roots) of dominant tree species in three tropical montane
forests on Mt. Kinabalu, Borneo, to investigate changes in root properties along a soil P availability gradient.
We found at the community level that root phosphatase activity and specific root surface area (root surface area per gram root
biomass) increased, and root diameter decreased, with decreasing soil P availability at the community level, and the relationshipwas not changed in general even if we focused on a single tree species distributed across multiple study sites that differ in soil
P availability. Root acid phosphatase was significantly positively correlated with specific root surface area, and negatively correlated with root diameter, suggesting that finer roots have higher phosphatase activities. Furthermore, we compared root acidphosphatase activity with leaf P concentration of a given tree species, and found a significant negative correlation between them.
The significant correlation suggested that root P-acquisition properties could influence leaf P concentration, and/or vice versa.
Belowground properties (i.e., root P-acquisition properties) might be directly/indirectly linked to aboveground properties (i.e.,leaf P concentration) of a tree individual.
In conclusion, the root physiological and morphological properties change along a gradient of soil P availability in the tropicalmontane forests. In addition, the changes in the root properties are coordinated with the changes in leaf P concentrations. The
adaptive changes in the aboveground and belowground properties along the soil P gradient could contribute to the maintenanceof forest productivity in the tropical montane forest in Borneo.
Keywords: Phosphatase activity, Plant-soil interactions, Tree roots, Root surface area, Soil phosphorus availability, Tropicalmontane forests

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MIS03-04

Room:104

Time:May 27 11:00-11:15

Distinctive pools and chemical species of phosphorus among density fractions of allophanic and non-allophanic Andisols
TAKAMOTO, Akira1 ; HASHIMOTO, Yohey1∗ ; WAGAI, Rota2 ; ASANO, Maki3
1

Tokyo University of Agriculture and Technology, 2 National Institute for Agro-Environmental Sciences, 3 University of Tsukuba

Andisols with abundant aluminum (Al) and iron (Fe) oxyhydroxides are characterized by a high phosphorus (P) retention
capacity. Such property leads to a significant inhibition of plant growth in Andisols unless properly managed. Andisols areclassified into two types in accordance with the difference in the clay mineral compositions. One type is referred to as allophanic
Andisols, in which allophane and imogolite are present in the clay fraction. The other type is referred to as non-allophanic
Andisols, in which Al- and Fe- humus complexes and 2:1 phyllosillicates are predominant. Based on the result of chemical
extraction, it has been suggested that these clay minerals appear to contribute to P retention capacity of Andisols. Separating
the different soil minerals in accordance with their density can limit the number of P-bearing phases, facilitating characterizationof species and accumulating pools of P. The objective of this study was to characterize the species and distinctive pools of P
in allophanic and non-allophanic Andisols using density separations in combination with sequential extraction and solution 31 P
nuclear magnetic resonance (NMR) spectroscopy.
Allophanic and non-allophanic Andisols collected from Tsukuba and Osaki, respectively, were fractionated by sodium polytungstate into five density levels including 1.6-1.8, 1.8-2.0, 2.0-2.25, 2.25-2.5, and >2.5 g cm−3 . Phosphorus in each density
fraction was extracted sequentially by deionized water, 0.5 M NaHCO3 , 0.1 M NaOH and 1.0 M HCl. After the extracts were
filtered, the concentration of inorganic P (Pi ) in all fractions was determined colorimetrically with a molybdenum blue method.
The concentration of total P (Pt ) in each fraction was determined by the same method after the solution was treated using H2 SO4 persulphate digestion. The concentration of organic P (Po ) was calculated as the difference between Pt and Pi of each fraction.
The total concentration of P was similar in the allophanic and non-allophanic Andisols (6.2 g kg−1 ). A large proportion of Pi
and Po in the bulk and each density fraction was extracted by NaOH, indicating that P in both Andisols was mainly associatedwith Al and Fe (oxy)hydroxides. The density fraction that accumulates P was contrastingly different between allophanic andnon-allophanic Andisols where over 90% of Pi and Po in the former was accumulated in the >2.0 g cm−3 fraction, whereas
about 70% of Pi and Po in the latter was found in the <2.0 g cm−3 fraction. According to the 31 P-NMR analysis, ortho-P
monoesters were the primary organic P species for the allophanic and non-allophanic Andisols, although it was 2-folds moreabundant in the latter than the former. In the non-allophanic Andisols, myo-inositol hexakisphosphate, an ortho-P monoester,
was accumulated in the 1.8-2.25 cm−3 fraction.

Keywords: phosphorus, NMR, chemical speciation

1/1

MIS03-05

Room:104

Time:May 27 11:15-11:30

Influence of feral goat and seabird activities on chemical properties of surface soils on an
oceanic island in JapanHIRADATE, Syuntaro1∗ ; MORITA, Sayaka1 ; HATA, Kenji2 ; OSAWA, Takeshi1 ; SUGAI, Kyoko2 ;KACHI, Naoki2
1

National Institute for Agro-Environmental Sciences, 2 Tokyo Metropolitan University

Ogasawara Islands, subtropical oceanic islands in north-western Pacific of Japan, have been listed as Natural World Heritage
by UNESCO since 2011 because of their valuable ecosystems sustaining many indigenous spices including plants and snails.
Nakoudojima Island and its peripheral reefs are the important components of the heritage, but the island has been exposed tothe serious influence of soil erosion damaging the indigenous ecosystems. Many areas on the island have been covered withnative forests before the introduction of goats (Capra hircus ) of ca. 200 years ago. The introduced goats had been naturalizedon the island since 1945 at the latest, and they had destroyed the native vegetation by grazing and trampling, resulting in a shiftof the native forests into grasslands and loss of the surface soils. To fix the problem, all the feral goats on the island had been
eradicated in 1999. Although the recovery of seabird nesting for brown booby (Sula leucogaster ) and wedge-tailed shearwater(Puffinus pacificus ) has been observed, the soil erosion has still been serious and plant biomass has been very low in some placeson the island at least in 2014.
To conserve the indigenous natural ecosystem and help the recovery of the natural vegetation, we conducted field survey andinvestigated the chemical properties of surface soil (0 ‐ 5 cm) and aboveground plant biomass in relation to the topography ofthis island. Under grassland vegetation where influence of feral goat would have been severe, the chemical properties of surface
soils, such as soil pH value, total C and N contents, exchangeable acidity, and plant-available phosphate (Bray II P), were highlyvariable even in a small area. By comparing soil profile characteristics under natural and disturbed vegetation and distribution
patterns of these soils on the island, the changes in the soil chemical properties were reasonably assigned to the effect of soil
erosion caused by feral goats for increased soil acidity (mainly found in inland valley area) and to the effect of seabird activities
for increased Bray II P and soil acidity (mainly found in outer rim area with high altitude). It was also clarified that the high soil
acidity was significantly related to the low productivity of plant biomass. Soil erosion would have removed surface soils having
weak acidity and exposed subsoils having strong acidity to the ground surface, resulting in inhibition of plant growth and delayof vegetation recovery. Based on the findings obtained in the present study, several options were proposed to stop soil erosion
and to recover the vegetation, although careful preliminary examination would be necessary for applying them.
Keywords: Nakoudojima Island, natural world heritage, plant-available phosphate in soils, soil exchangeable acidity, exotic
species, soil erosion

1/1

MIS03-06

Room:104

Time:May 27 11:30-11:45

Pedogenesis of tephra-derived soils in JapanINOUE, Yudzuru1∗
1Graduate School of Science and Engineering, Saitama University

In Japan, there are still 110 active volcanoes today, which occupy approximately 7 percent of those in the world. Some
volcanoes in Japan are erupting now and continuing deposition of tephras on the land surface. They will become the parent
material of the soils. Of the soils in Japan, tephra-derived soils ‘Kuroboku soils (Andosols/Andisols)’ are the second-most common group under the main classification scheme in usage, the Unified soil classification system of Japan (FCSCN, 2003). Many
tephra-derived soils are distributed near and around numerous volcanoes in Japan. Tephra-derived soils composed of black soils(abundant humic soils), brown soils (mainly brown loamy soils: including tephric loess and loess), and Regosols (Tephric) (FAO,
2006) (very weakly developed mineral soils). It has been suggested that the black soils develop when the supply of organic
matter by Gramineae grass exceeds the rate of addition of tephra parent materials (Inoue, 2002). It is usually stabilized by theformation of allophane and Al- (or Fe-) humus complexes. Origin of the abundant humus is confirmed by the high correlation
between organic carbon content and phytolith content of Gramineae grass (Inoue et al., 2000; 2001 etc.). Most of the Japanese
Holocene tephra-derived soils are rich in humus. Older buried humic-rich soils can also be observed in Late Pleistocene tephrasoil sequence in Japan (Inoue et al, 2011a). One of the tephra-derived soils ‘brown soils’ having poor in humus include anaeolian-reworked tephras (tephric loess; Pullar and Pollok, 1973) as main parent material. Tephric loess occurs in tephra-soil
sequences in Japan and is also interlayered with ‘background’ loess derived from long-term (continuously-deposited) loess (aeolian dust) deposition from Gobi, Taklamakan desert, and the loess plateau in central Asia (Inoue and Naruse, 1987). This loessis barely observed in Japan. Sase and Hosono (1996) shows that pedogenesis of brown soils occurs under the forest vegetationby using vegetation changes from phytolith composition in tephra-soil sequence.
Pedogenesis includes both ‘topdown’ and ‘upbuilding’ models (Almond and Tonkin, 1999; Lowe, 2000; Inoue, 2001). Topdown pedogenesis is ‘classical’ soil formation that occurs by leaching, illuviation, and other processes that form andic materials
with horizons developing in a downward-moving front. Upbuilding pedogenesis operates where the soil forms while additions
to the soil surface of such materials as tephra or loess occur. If additions are sufficiently slow ̶ typically as thin incremental
deposits in distal areas ̶ then topdown pedogenesis continues while the land surface slowly rises (referred to as ‘developmental
upbuilding’). If additions are thick or frequent, as typically occurs nearer volcanic sources, then the antecedent soil is buried andisolated, and soil formation begins again on the new materials at the land surface (‘retardant upbuilding’) (Inoue et al., 2011b;
Lowe et al., 2008). The profile character is thus determined by the interplay between the rate at which tephras are added to
the land surface and topdown processes. Understanding Andosol/Andisol genesis thus often requires a stratigraphic approachcombined with an appreciation of buried soil horizons and polygenesis (Lowe and Tonkin, 2010). The terms ‘developmental
upbuilding’ and ‘retardant upbuilding’ were first used by Johnson and Watson-Stenger (1987) and Johnson et al. (1990) as partof their dynamic-rate model whereby soils evolve by ‘ebb and flow’ through time (Schaetzl and Anderson, 2005). As mentioned
above, most of the tephra-derived soils in Japan are formed by upbuilding pedogenesis and may be described as multisequal soils.
At the present day, theories concerning pedogenesis of tephra-derived soils in Japan are changing from long-established theories. The soils in the regions having numerous active volcanoes occur distinctive pedogenesis unlike in non-volcanic regions.
Keywords: soil, tephra, pedogenesis, tephra-derived soil, Andosols/Andisols, phytolith

1/1MIS03-07

Room:104

Time:May 27 11:45-12:00

Effect of climate on vegetation-soil system after volcanic ash deposition 7300 years ago
on Yakushima Island
MUKAI, Mana1∗ ; AIBA, Shin-ichiro2 ; KITAYAMA, Kanehiro1
1

Graduate School of Agriculture, Kyoto University, 2 Guraduate School of Science and Engineering, Kagoshima University

Today’s vegetation on Yakushima Island varies across elevations. Yakushima Island is covered with the Akahoya volcanic ash
being derived from the Kikai Caldera about 7300 years ago. The eruption was so destructive that today’s vegetation was established through primary succession after the volcanic eruption. The five independent state factors, which are climate, topography,organism, parent material and time, must have intricately affected the formation of the current soil-vegetation system. Among
five factors, Climate including precipitation and temperature must have strongly affected the formation of today’s vegetation.
The purpose of this study is to clarify how climate has influenced the vertical distribution of the vegetation during 7300 years byinvestigating the relationships between soil mineral nutrients and nutrient-use efficiencies of the vegetation.
In this study, seven permanent study plots were set up along the elevation gradient. Soil samples (0-10, 10-20 cm depth) wererandomly collected from each forest. We determined phosphorus (P) fraction in the soils following the method of Tiessen &Moir (1993). Inorganic nitrogen (N) and exchangeable cations were determined by using the extract with 1.5N KCl. P and N incollected fresh litter were determined by using Kjeldahl digests with concentrated H2 SO4 and H2 O2 . We calculated mean valuesof soil elements in each forest and examined the relationships with temperature and precipitation. Temperature and precipitation
data were cited from the publicized national numerical average data. Both data do not correlate with each other.
Soil total P (Pt) and inorganic N had a significant positive correlation with temperature but not with precipitation. Occluded-P(Occl-P) had a strongest negative correlation with temperature among P fractions. On the other hand, exchangeable cation had astronger negative correlation with precipitation than with temperature. Pt and inorganic N had a negative correlation with eachnutrient-use efficiency.
P is an essential element whose primary source is the weathering of minerals in parent materials. Considering that the pedogenesis on Yakushima Island is merely 7300 years, P in current topsoil must be derived primarily from the Akahoya volcanicash. According to Walker & Syers (1976), Pt and acid extractable Ca-P in soils decrease along a pedogenesis chronosequence.
In addition, Occl-P being unavailable to plants, increases with the progress of weathering. However, our results indicate that
Occl-P is higher at lower elevations and that Pt is lower at higher altitudes where the intensity of weathering is low because of
low temperature. Moreover, Ca-P, which is a primary mineral P in parent materials, decreases with increasing elevation. These
results are not in accordance with the model of Walker & Syers (1976). We suggest that temperature and precipitation have
intricately affected the weathering and leaching of nutrients, and influenced the formation of P fractions.
At higher elevation sites, annual rainfall exceeds annual evapotranspiration. As soil organic matter is decomposed, soils will
be reduced by respiration of microbes and soil pH will be lower. Consequently, at higher elevation, acid extractable Ca-P andaluminum (Al) and iron (Fe) are dissolved and then P bound to Al and Fe are leached from topsoil. At lower elevation sites,
Occl-P is formed but Pt is also kept and soil inorganic P as a plant available form is higher. Soil inorganic N is also higher atlower elevations probably due to the direct effects of temperature as well as the indirect effects via P availability; the two effects
are, however, inseparable in this study.
In conclusion, the availability of P and N varied across elevations primarily due to temperature, which in turn determined Pand N-use efficiencies of the forests. Today’s vegetation is, thus, formed via the effects of soils as a function of temperature in
addition to the direct effects of temperature on plants.
Keywords: Phosphorus, Volcanic Ash, Nutrient-use efficiency, Climate, Pedogenesis

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MIS03-08

Room:104

Time:May 27 12:00-12:15

Origin of mica in Allophanic Andosols in Japan and its role as a radiocesium fixing material
NAKAO, Atsushi1∗ ; NAKAO, Aya2 ; TANAKA, Ryoji3 ; YANAI, Junta1
1

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 2 Faculty of Life and Environmental Sciences, Kyoto Prefectural University, 3 Institute for Study of the Earth’s interior, Okayama University
The accident at the Fukushima Daiichi Nuclear Power Plant in March 2011 has turned attention to the fate of radiocesium(RCs) in soils in Japan. Allophanic Andosols are common soils in Japan, which generally have clay mineralogy rich in low crystalline minerals such as allophane and imogolite. Since RCs is not adsorbed strongly on these minerals, Allophanic Andosols are
assumed to have very low RCs retention ability. The objective of this study is to elucidate the relationship between RCs retention
ability and mineralogical properties of Allophanic Andosols in Japan. We hypothesized that trace amount of micas are deposited
as a loess component even in Allophanic Andosols, which control the RCs retention ability.
Twenty-three soil samples were collected from a plow layer (0-15 cm) of either paddy or upland fields distributing at alluvial
plains in Hokkaido, Tohoku, Kanto, and Kyusyu districts, representative areas of Allophanic Andosols. Particles with a size of
2-20 µm and <2.0 µm were fractionated from the soils by sedimentation method. RCs retention ability for each particle wasrepresented by the Radiocesium intereception potential (RIP). Quartz content was estimated by random powder X-ray diffraction
analysis for 2-20 µm particles with adding α-Al2 O3 as an internal standard. Mica content in 2-20 µm particles was estimated by
the amount of potassium extracted by fusion with NaHSO4 , whereas that in <2.0 µm particles was estimated by K extracted bydigestion with HF-HClO4 . Quartz was isolated from 2-20 µm particles by the selective dissolution with H2 SiF6 , and then δ 18 O
value for the isolated quartz was determined to estimate the origin.
The RIP value for 2-20 µm and <2 µm fractions was 1.7±0.8 mol kg−1 and 2.6±1.3 mol kg−1 , respectively. Mica-K content
in the respective fractions was 3.2±1.3 g kg−1 and 3.4±1.7 g kg−1 . These values are considerably small compared with thosefor fine particles in non-volcanic soils. The RIP positively correlated with mica-K content for each fraction, indicating that RCs
retention ability is mainly controlled by the amount of micas, in spite of its minority as a mineral component. The mica-K content
is proportional to the quartz content, suggesting that the origin of these minerals would be the same. Furthermore, δ 18 O value
for the isolated quartz was +14.8 ‰ on average with a range of +10.8 to +16.1 ‰, which is clearly higher than those of volcanic
materials while similar to those of Chinese loess. Thus, this study strongly indicated that the RCs retention ability of Allophanic
Andosols is largely controlled by loess-derived micas.
Keywords: loess, oxygen isotopic analysis, Radiocesium Interception Potential, Allophanic Andosol, mica

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MIS03-09

Room:104

Time:May 27 14:15-14:30

Relevant approaches and strategies for investigation on the nitrogen saturation in forested
catchments
OHTE, Nobuhito1∗ ; ISOBE, Kazuo2 ; TOKUCHI, Naoko1
1

Kyoto University, 2 University of Tokyo

After the Industrial Revolution, global scale nitrogen (N) cycle has drastically been altered by increase of anthropogenic N
emissions to the atmosphere. Inorganic N concentration in atmosphere and their depositions reached unprecedented level in
Europe, northeast United States and northeast Asia. These have caused ”Nitrogen Saturation” in the forested areas within thehigh N deposition regions of Europe, North America, China and Japan. Mechanisms of nitrogen saturation have previously
been studied from biogeochemical point of view. N dynamics in catchment-scale, however, could not be described sufficiently,
because geographical variations of catchment characteristics such as climatic and hydrologic properties are generally large andtheir effects provide various aspects of responses in high nitrogen depositions. We propose new strategies based on multi-aspects
approach combining microbial ecology and catchment hydrology to reconstruct the mechanistic understandings on previously
reported ecosystem level biogeochemical responses to the environmental changes such as high N inputs. Combined applicationsof novel isotopic tracer techniques and newly advanced functional gene analysis onto the multiple forest landscapes will provide
us insightful information on spatiotemporal heterogeneity and non-linear responses of N dynamics related to the N saturation
phenomena in forest catchments.
Keywords: forested catchment, nitrogen saturation, microbial ecology, isotope tracer, hydrological processes

1/1MIS03-10

Room:104

Time:May 27 14:30-14:45

Carbon and nitrogen transformation and their driving microorganisms in paddy soil, asassessed by meta-transcriptomics
TABUSHI, Yoko1∗ ; ITOH, Hideomi2 ; SHIRATORI, Yutaka3 ; ISOBE, Kazuo1 ; OTSUKA, Shigeto1 ;SENOO, Keishi1
1

Graduate School of Agricultural and Life Sciences, The University of Tokyo, 2 National Institute of Advanced Industrial Scienceand Technology (AIST) Hokkaido, 3 Niigata Agricultural Research Institute
Paddy soil undergoes drastic change in redox potential along water management during rice cultivation period. After waterlogging, various reductive biochemical processes, including denitrification, metal and sulfate reduction, and methanogenesis canoccur in the soil, leading to generation of reduced soil layer. On the other hand, thin oxidized layer is present at soil surface,where oxidative biochemical processes such as nitrification and methane oxidation can occur. These oxidative and reductive
reactions play central role in material transformation in paddy soil and are closely related to soil fertility, rice growth and surrounding environment.
We carried out meta-transcriptomic analysis of oxidized and reduced soils collected from Niigata paddy field during ricecultivation period. Whole microbial communities, quantity and diversity of transcriptome involved in carbon and nitrogen transformation in the soils were investigated by rRNA and mRNA analyses. Active microbial communities, possible carbon andnitrogen transformation, microbial players involved in the transformation, and their temporal transition in the paddy soil will bereported.
Keywords: soil microbial communities, metatranscriptome, bioinformatics, paddy soil, CN cycle

1/1

MIS03-11

Room:104

Time:May 27 14:45-15:00

Agricultural abandonment influences the ecosystem carbon pools
SHIMODA, Seiji1∗ ; WAGAI, Rota2
1

NARO Hokkaido Agricultural Research Center Memuro, 2 National Institute for Agro-Environmental Sciences

Land abandonment, which is increasing globally, has significant impact on terrestrial carbon (C) budget, vegetation, andbiodiversity. Invasive alien plants often outcompete native plants after agricultural abandonment, which can lead to the alterationin ecosystem C and nutrient balance. Perennial plants have been an exceptionally successful invader in agricultural abandoned
fields around the world. Irrespective of soil nutrient status, alien plants rapidly dominates abandoned agricultural fields in thetemperate regions. While negative impact of alien plants on local and regional biodiversity is well established in conservation
ecology, its impact on C sequestration potential is much less studied. Paddy fields used for rice (Oryza sativa L.) production
are the dominant human land-use systems for a long time throughout Japan. Japan has increased to nearly 10% of the total
cultivated land area. The aim of the present study was to investigate how the rice paddy abandonment influenced the storage of
C in ecosystem components during the secondary succession over decadal time scale.
If paddy fields, the typical agricultural land in Japan, are left abandoned, the amount of soil C in the abandoned fields up to
20 years after being abandoned is lower than that of paddy fields under cultivation. If the field is abandoned for a long time, theamount of soil C increases due to organic matter from weeds, but it is considered that the process will take more than 20 years.
We hypothesize that the invasion of alien plants to the abandoned paddy fields enhances ecosystem C storage by their high N use
efficiency and high productivity. As results, the temporal change in soil C was similar among vegetation type through amount of
input C was similar in present study.

Keywords: land use change, paddy, grassland, invasive alien plants, litter

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MIS03-12

Room:104

Time:May 27 15:00-15:15

Responses of organic carbon in a variety of soils controlled by temperature and cellulose
supply
IIMURA, Yasuo1∗
1

The University of Shiga Prefecture

Carbon stored in the upper meter of mineral soils is estimated to be 2500 Gt, which is approximately 3.3 times the size of theatmosphere and 4.5 times the size of the vegetation. Therefore, soil organic matter is often considered as a significant carbon
0
reservoir on the earth s surface. Although changes in soil organic carbon contents (by natural or anthropogenic causes) have asignificant impact on the global carbon cycle, the mechanisms of soil organic carbon stabilization and destabilization and thefactors controlling these mechanisms are not very well understood. We thus focused on the response of organic carbon in avariety of soils (black soil, brown soil, and red-yellow soil, etc.) controlled by temperature and fresh carbon (cellulose) supply.
In addition, we considered the factors controlling the temperature sensitivity and priming effect.

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MIS03-13

Room:104

Time:May 27 15:15-15:30

Organic matter stabilization in Andisol and Ultisol revealed by isotopic tracer experimentand density fractionation
HAYAKAWA, Chie1∗ ; WAGAI, Rota2 ; INAGAKI, Yoshiyuki3 ; ASANO, Maki2
1

Univ. Tokyo, 2 NIAES, 3 FFPRI

For predicting C cycling in terrestrial ecosystem, dynamics of organic matter (OM) in soil can be a large component that
increases uncertainty. Once OM is supplied into soil system mainly as plant detritus and root exudates, OM is decomposed by
microorganisms and a proportion of OM is stabilized through association with soil mineral particles. The OM in soils has a wide
range of size, density, and chemical reactivity. Organo-mineral particles of heavy-density fraction are highly resistant against
microbial degradation compared to mineral-free OM (i.e., plant detritus and low-density fraction). The high C sequestration
capacities of soils (e.g., Andisol) are hypothesized to be regulated by incorporation rates of microbial-processed OM into heavier
fraction. To test this hypothesis, we conducted incubation experiment using tracer to quantify the pool sizes, influx and efflux
rates, and mean residence times (MRTs) of different density classes.
Different types of soils were sampled from two agricultural lands; a volcanic-ash soil (Andisol) from Japan and a highlyweathered tropical soil (Ultisol) from Indonesia. The incubation experiments were carried out after addition of 13 C-labelled
glucose (99 13 C atom%, 0.1915 mmol 13 C g 1soil as solution) or 13 C, 15 N-labeled glutamic acid to the soils (2-mm sieved, 5 g
dry weight). The soils were incubated for 276 d at 30oC and 50% water holding capacity. After the incubation, soil was separated
into three fractions according to its density using sodium polytungstate as heavy liquid: low (<1.8 g cm−3 ), middle (1.8-2.25 g
cm−3 for Andisol, 1.8-2.5 g cm−3 for Ultisol), high (>2.25 g cm−3 for Andisol, >2.5 g cm−3 for Ultisol) density fractions. We
measured the mass, isotopic ratios (13 C/12 C, 15 N/14 N) and total C and N concentrations of the density fractions as well as theamount of CO2 respired during the incubation by alkali trap method. We also measured the specific surface areas (SSA) of soil
minerals and the concentrations of Al, Fe oxides/hydroxides.
For both soils, ca. 70 to 80 % of added 13 C were mineralized to CO2 within 1 month after substrate addition. The density
fractionation showed that 13C recovery in the low-density fraction was low (0.5 - 3.8%) throughout the incubation period.
The 13 C recovery within the mid- and high-density fractions was greater than 20%. This indicates that labile substrates wereimmediately incorporated into the mid- and high-density fractions through microbial processing in the both soils. The highest
13
C recovery was observed in the mid-density fraction of Andisol and in the high-density fraction of Ultisol, respectively. MRTsof 13 C in the density fractions positively correlated with SSAs for respective soil types. This can be explained by differences inmineralogy which contribute to OM stabilization through sorption; short-range-order minerals (e.g., allophane and imogolite) inAndisol and iron oxides in Ultisol, respectively. Our results support the hypothesis that newly-added OM is stabilized throughassociation of microbial metabolites with mineral particles. However, dominant density class and turnover of stabilized OM
could be variable depending on soil types and clay mineralogy with high specific surface areas.

Keywords: 13C-glucose, 13C, 15N-glutamic acid, Andisol, Ultisol, organo-mineral particle

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MIS03-14

Room:104

Time:May 27 15:30-15:45

Changes in the structure and function of tannins in natural environments
MAIE, Nagamitsu1∗
1School of Veterinary Medicine, Kitasato University

Tannins are polyphenols that are contained in plants where they can account to 20% of the plant dry weight depending on its
species and organs. Tannins are known to bind to proteins, making insoluble complexes that are resistant to microbial degradation. While tannins are considered to play various roles in ecosystems, we are not fully understand the dynamics and functions oftannins in them. Here, I would like to present the changes in the structure and function of tannins in water and soil environments.
Furthermore, their possible influence on nitrogen cycling in mangrove ecosystems will be proposed.
Molecular structure and protein binding ability of CT changes during the decomposition of foliage (Maie et al. 2003)
Tannins are classified into two subgroups, condensed tannins (CT) and hydrolysable tannins (HT). CT are mixtures of polymers of flavan-3-ol units with different degrees of polymerization and mostly hydroxyl substitutions. Molecular structure of CT
in foliage changes during the decomposition of foliage. CT molecules composed of prodelphinidin unit (PD), which has morehydroxyl groups than procyanidin (PC), are more susceptible to structural changes. Structural change of CT accompanied withthe decrease of protein-binding ability.
Tannins are important source of DOM leached from litter, especially at the early stage of decomposition (Nishimura etal. 2012)
Dissolved organic matter (DOM) leached from litter may contain tannin-derived materials. Since tannins are water-soluble
and has wide structural variety among different species, DOM composition in leachate is most diverse at the early stage of the decomposition, but converge into relatively similar composition by time when lignin-degradation products become a major sourceof DOM.
Tannins-protein complex may contribute to nitrogen cycling in mangrove ecosystem, acting as a delayed release fertilizer (Maie et al., 2008)
Fate of CT leached into water environments can be variable. They may aggregate in saline water, adsorb to sediment, andcomplex with proteins. CT change their chemical structure quickly in water, becoming ”invisible” to analytical window. CTprotein complexes are refractory to microbial degradation, but photo-reactive. By exposing CT-protein complexes to sun light,
proteins can be released into water. In mangrove estuary, a large amount of tannins and proteins could be released into water in a
relatively short period when leaves fall into water. CT might be contributing to preserve N in mangrove ecosystem, by acting asa delayed release fertilizer.
References
Maie et al. 2003. Soil Biology & Biochemistry 35, 577-589.
Maie et al., 2008. Limnology & Oceanography 53, 160-171.
Nishimura et al. 2012 J. Environ. Quality 41, 823-833.
Keywords: dissolved organic matter, forest ecosystem, mangrove estuary, nitrogen cycling, protein binding ability, structuralchange1/1

MIS03-15

Room:104

Time:May 27 15:45-16:00

Nutrient release during biodegradation of organic horizons in the Siberian taiga underlain
by continuous permafrost
KAWAHIGASHI, Masayuki1∗ ; PROKUSHKIN, Anatoly2 ; SUMIDA, Hiroaki3
1Graduate School of Urban Environmental Sciences, 2 Sukachev Institute of Forest, Rossia Academy of Science, 3 College of
Bioresource Sciences, Nihon University
Organic horizons under the Siberian taiga forest have a significant role of nutrient supply for plants through their biodegradation processes and of heat insulation for permafrost table underneath shallow mineral soil layer. Lower weathering stages ofmineral soils underlain by permafrost have lower potential of soil nutrient retention and release, indicating that summer growing
vegetation can expect to fill their nutrient requirements released by organic matter degradation. Decomposed organic matter can
supply mineral and biogenic nutrients to plants and microorganisms. Organic horizons often suffer from frequent forest fire in
the Siberian forest. Forest fire moves through organic horizon on the ground because of low tree density in the forest stands.
Relatively low burning temperature can leave organic residue on the forest ground. Ground temperature during forest fire is a
key variable for changes in properties of organic horizons. Solutes release potential and biodegradability of organic horizons
was evaluated in this study along with heating temperature gradient. To estimate the effect of forest fire on the biodegradation
processes of organic horizons, we prepared organic horizon samples heated between 65o C and 550o C under a relatively oxygen
limited atmospheric condition.
A sampling site located in the Evenkia province in the central Siberia. Soils are classified as Oxyaquic Cryosols or Gelic Cambisols according to the WRB classification system. Organic horizon samples were taken depending on hummock topography.
Air-dried and powdered samples were heated in a muffle furnace between 105 and 550o C for 15minites. Organic residues wereapplied to incubation experiment to evaluate biodegradation and solutes release. Biodegradation was evaluated from mineralization rates being calculated using temporal changes in CO2 concentration during an incubation experiment and total organiccarbon content in heated solid samples. During the same incubation time course, biogenic elements released from the heatedsamples were determined after water extraction by ICP-AES, Ion-chromatography and TOC-L with total nitrogen module unit.
Solutes release from heated organic horizon samples by water saturation was different between samples depending on theheating temperature. Larger amount of nutrient elements were released from organic horizons taken from trough points on thehummock. Samples heated at 250o C released the highest amount of solutes mainly dominated by dissolved organic C. Basic
cations and major anions including phosphate, nitrate and chloride were also largely released from the sample heated at 250o C.
Higher the heating temperature was, solutes concentration was lower. Solution pH was higher with increasing the heating temperature. The ratios of carbon to nitrogen in solid samples were decreased with increasing the heating temperature. The temperaturedependence of solutes release was different along with hummock topography.
The maximum mineralization (%) was approximately 4% of total C in samples heated at lower temperature below 180o C. The
mineralization rate was not largely different between samples, indicating that the biodegradation process in all heated samples
mainly depends on the amounts of easily decomposable carbon source, such as DOC. There are significant correlations between
the maximum mineralization rate and DOC or inorganic N. Solutes mainly consisting of nutrients and energy source for microorganisms can be a significant controlling factor for the biodegradation process of organic horizons. The difference in solutes
composition and the biodegradation were mainly depending on heating temperature of organic horizons. Heating temperatureduring a forest fire is an important parameter controlling the further degradation of organic matter in organic horizons and thefate of carbon dynamics in the boreal permafrost affected forest region.

Keywords: Forest fire, Biodegradation, Continuous permafrost terrain, plant nutrition, burned temprature, humic substances

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MIS03-16

Room:104

Time:May 27 16:15-16:30

Variation in physical composition of soil organic matter in black spruce forests within aslope in Interior Alaska
TORIYAMA, Jumpei1∗ ; TANAKA, Ayumi O.1 ; MORISHITA, Tomoaki1 ; MATSUURA, Yojiro1 ;HINZMAN, Larry D.2
1

Forestry and Forest Products Research Institute, 2 International Arctic Research CenterIn boreal region, rapid climate warming compared to lower latitude region can accelerate decomposition of soil organic matter(SOM) and, together with an increase in active layer depth, shift patterns of nutrient use and growth of boreal forests. In
discontinuous permafrost region of Interior Alaska, black spruce (Picea mariana ) grows in environments with various active
layer depths and different degree of nutrient limitation. These environmental gradients can also be obtained from different
positions in a single slope where climatic condition and fire history are similar. To clarify accumulation pattern of SOM and its
relationship to tree growth and slope position, we set a transect plot of 1.5km-long in black spruce forests in Caribou Poker Creek
Research Watershed. Tree growth rate at lower altitude (250 m) with shallow active layer in growing season is low compared tothat at higher altitude (450 m) with deep active layer. We collected samples from organic layers and mineral soil horizons in 14
soil profiles. The thickness of organic layer ranged 7 to 45 cm and was not correlated with altitude. Soil samples are separated
into light and heavy fractions by density fractionation approach. The light fraction of topsoil (surface horizon of mineral soil)
accounted for 269 g kg−1 of soil mass and 598 g kg−1 of soil organic carbon in average. The relationship between light fraction
content of topsoil and slope position was unclear. In the session, we focus on the 15 N natural abundance of SOM and othercomponents in the forest ecosystem and discuss the nitrogen cycling in black spruce forests with different growth rates.
Keywords: black spruce, soil organic matter, density fractionation

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MIS03-17

Room:104

Time:May 27 16:30-16:45

Controls over turnover of organic matter and nutrients in permafrost soils
FUJII, Kazumichi1∗ ; MATSUURA, Yojiro1 ; FUJII, Soichiro2 ; INAGAKI, Yoshiyuki1 ; OSAWA, Akira2
1

Forestry and Forest Products Research Institute, 2 Graduate School of Agriculture, Kyoto University

Introduction: Plant productivity on permafrost soils is limited by nutrient supply from organic matter. Organic matter decomposition and nutrient release can be limited by cold climate, flooding, and recalcitrance of bryophytes (lichen and moss).
Plant-soil association (white spruce on mineral soil, black spruce on organic soil, and shrub tundra on lowland soil) suggests the
hypothesis that plant acquisition strategies for nutrient (esp., amino acids/inorganic N) can be matched by nutrient supply fromsoil organic matter. To test this, turnover of organic matter and nutrient release was investigated for three types of ecosystems in
Northwest Territory, Canada; white spruce forest (WSF) on the upland soil derived from glaciofluvial sands, black spruce forest(BSF) and tundra (TND) in lower position on fluvial sediments.
Methods: We measured soil organic carbon (SOC) storage [organic and mineral soil layers (0 to 30 cm)], soil temperatureand moisture, aeration index [Eh, free Fe oxides (oxalate-extractable Fe)] of soils, and the decomposition rates of litter (lichen,moss, and root litter) and cellulose filter paper buried in the soils. Regarding soil N dynamics, the concentrations of organicand inorganic N in soil solution (zero-tension lysimeter) were measured. Root uptake of dual-labeled (13 C, 15 N) glutamic acid,15
N-labeled ammonium, and 15 N-labeled nitrate was measured 24 h after spike of mixture solution.
Water dynamics: Episodic flooding events were observed following spring snowmelt at all sites. Rapid snowmelt and waterpercolation enhanced aeration in the sandy soil profile of WSF, while the BSF and TND soils were saturated by water flooding
on impermeable permafrost layer (30 cm deep) even in summer. The seasonal cycles of reducing- and oxidizing- conditions wererecorded as accumulation of free Fe oxides in the soils.
C dynamics: The C stocks in the organic and mineral soil layers were greater in TND (188 Mg C ha−1 ) and BSF (207-237
Mg C ha−1 ) than in WSF (37 Mg C ha−1 ). When the regression analysis was conducted for 15 soil profiles, there was a positive
correlation between SOC storage and free Fe oxide concentration. The high concentrations of free Fe oxides in soils appeared
to be an index of poor drainage and high SOC storage. Mass loss rates of cellulose filter paper, lichenous litter, and root litter
followed the order: WSF>TND>BSF. Water flooding and cold climate retarded decomposition of organic matter in BSF and
TND. The development of hummocky micro-topography, which was recorded as the tilting of drunken forest, resulted in accumulation of sparingly-decomposable lichen and moss debris in BSF. The warmer and aeration conditions in sandy upland soil of
WSF enhanced turnover of organic matter.
N dynamics: Dissolved organic N is abundant in soil solution at all sites. Nitrogen species in soil solution was dominated bynitrate and ammonium ions in TND soil, while it was ammonium in WSF and BSF soils. Regarding N uptake by plants, TND
plants (shrub birch and grasses) preferentially absorb inorganic N (ammonium and nitrate), while white spruce and black spruce
could also utilize amino acid-N. Both C and N of amino acids were assimilated by white spruce roots, while only ammonium
was transferred to roots of black spruce probably after rapid mineralization by mycorrhizae or roots. N preference of plants is
consistent with the dominant N species in soil solution.
Conclusions:Water flooding as well as cold climate retarded turnover of organic matter in black spruce forest. Despite slow
turnover of organic matter, black spruce can utilize amino acids as well as ammonium. In warmer and aerated sandy soil, white
spruce can absorb both amino acids and inorganic N. In the lowland tundra soil rich in inorganic N, plants can absorb inorganic
N. This highlights the importance of considering plant-soil association to predict responses of ”sensitive” ecosystem to futurechanges in flooding, fires, and climate.

Keywords: permafrost, soil organic matter, dissolved organic matter, amino acids, microorganism

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MIS03-18

Room:104

Time:May 27 16:45-17:00

Possible future directions in soil and ecosystem research
WAGAI, Rota1∗ ; KOSAKI, Takashi2
1National Institute for Agro-Environmental Science, 2 Tokyo Metropolitan University

Better understanding of soil is fundamental to enhance sustainability of humankind, to conserve natural environment, and to
predict/manage future earth environment. Thus, soil scientists can and should play bigger role beyond the field of agriculturalscience and collaborate more with the scientists of other disciplines. We will discuss possible future directions that we could taketo better understand soil processes in earth system’s context.
Keywords: soil process, biogeochemistry, ecosystem, ecology, environmental science, earth surface processe

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