Curriculum Vitae
Wakana Ohashi, Ph.D.
E-mail: wohashi@u-shizuoka-ken.ac.jp
Homepage: https://w3pharm.u-shizuoka-ken.ac.jp/immunol/Employment
University of Shizuoka, School of Pharmaceutical Sciences: Associate Professor(2023-present)
Keio University, Faculty of Pharmacy: Lecturer (2020-2023)
University of Toyama, Graduate School of Medicine & Pharmaceutical Sciences:
Assistant Professor (2013-2020)
RIKEN Research Center for Allergy and Immunology: Research Scientist (2011-2013)
RIKEN Research Center for Allergy and Immunology: Research Associate (2008-2011)
RIKEN Genomic Sciences Center: Technical Staff (2001-2008)

Education
Ph.D. in Engineering, Yokohama National University (2011)
Master of Pharmaceutical Sciences, Kyoto University (2001)
Bachelor of Pharmaceutical Sciences, Toho University (1999)

Research Field of Interest
Microbiome, Metabolites, Mucosal Immunology, DOHaD

Selected Publication
1. Sakamaki I, Fukushi M, Ohashi W, Tanaka Y, Itoh K, Tomihara K, Yamamoto Y, Iwasaki
H. Sitafloxacin reduces tumor necrosis factor alpha (TNFa) converting enzyme (TACE)
phosphorylation and activity to inhibit TNFa release from lipopolysaccharide-stimulated
THP-1 cells. Sci. Rep. 11, Article number: 24154(2021)
2. Nakamura A, Kurihara S, Takahashi D, Ohashi W, Nakamura Y, Kimura S, Onuki M,Kume A, Sasazawa Y, Furusawa Y, Obata Y, Fukuda S, Saiki S, Matsumoto M, Hase K.
Symbiotic polyamine metabolism regulates epithelial proliferation and macrophage
differentiation in the colon. Nat. Commun. 2021 Apr8;12(1):2105 doi:10.1038/s41467021-22212-1
3. Nishida K, Hasegawa A, Yamasaki S, Uchida R, Ohashi W, Kurashima Y, Kunisawa J,Kimura S, Iwanaga T, Watarai H, Hase K, Ogura H, Nakayama. M, Kashiwakura J,Okayama Y, Kubo M, Ohara O, Kiyono H, Koseki H, Murakami M, Hirano T. Mast cells
play role in wound healing through the ZnT2/GPR39/IL-6 axis Sci. Rep., 9(1):10842(2019)

4. Ohashi W, Hara T, Takagishi T, Hase K, Fukada T. Maintenance of intestinal epithelial
homeostasis by zinc transporters. Dig. Dis. Sci. Mar 4. (2019)
5. Ohashi W*, Fukada T*. Contribution of zinc and zinc transporters in the pathogenesis of
inflammatory bowel diseases. J. Immunol. Res. 2019;8396878 (2019) (*: correspondingauthor)
6. Palikhe S, Ohashi W*, Sakamoto T, Hattori K, Kawakami M, Andoh T, Yamazaki H, Hattori
Y. Regulatory role of GRK2 in the TLR signaling-mediated iNOS induction pathway inmicroglial cells. Front. Pharmacol. 4;10:59 (2019) (*: corresponding author)
7. Sakamoto T, Ohashi W, Tomita K, Hattori K, Matsuda N, Hattori Y. Anti-inflammatory
properties of cilostazol: Its interruption of DNA binding activity of NF-kB from the Toll-likereceptor signaling pathways. Int. Immunopharmacol., 62;120-131 (2018)8. Kawakami M, Hattori M, Ohashi W, Fujimori T, Hattori K, Takebe M, Tomita K, Yokoo H,Matsuda N, Yamazaki M, Hattori Y. Role of G protein-coupled receptor kinase 2 inoxidative and nitrosative stress-related neurohistopathological changes in a mousemodel of sepsis-associated encephalopathy. J. Neurochem. 145(6);474-488 (2018)
9. Imaizumi T, Matsuda N, Tomita K, Ohashi W, Hattori K, Hattori Y. Activator protein-1
decoy oligodeoxynucleotide transfection is beneficial in reducing organ injury andmortality in septic mice. Crit. Care Med. (2018) May;46(5);e435-e442
10. Ohashi W*, Yamamine N, Imura J, Hattori Y. SKL2001 suppresses colon cancer spheroid
growth through regulation of the E-cadherin/b-Catenin complex Biochem. Biophys. Res.
Commun. 493(3);1342-1348 (2017) (*: corresponding author)
11. Misawa H, Ohashi W, Tomita K, Hattori K, Shimada Y, Hattori Y. Prostacyclin mimetics
afford protection against lipopolysaccharide/d-galactosamine-induced acute liver injury
in mice Toxicol. Appl. Pharmacol. 334;55-65 (2017)
12. Takashina M, Inoue S, Tomihara K, Tomita K, Hattori K, Zhao Q, Suzuki T, Noguchi M,Ohashi W, Hattori Y. Different effect of resveratrol to induction of apoptosis depending on
the type of human cancer cells Int. J. Oncol. 50(3);787-797 (2017)
13. Hattori M, Yamazaki M, Ohashi W, Tanaka S, Hattori K, Todoroki K, Fujimori T, Ohtsu H,
Matsuda N, Hattori Y. Critical role of endogenous histamine in promoting end-organ
tissue injury in sepsis Intensive Care Med. Exp. 4(1);36 (2016)
14. Ohashi W, Kimura S, Iwanaga T, Furusawa Y, Irie T, Izumi H, Watanabe T, Hijikata A,Hara T, Ohara O, Koseki H, Sato T, Robin S, Mori H, Hattori Y, Watarai H, Mishima K,Ohno H, Hase K, Fukada T. Zinc transporter SLC39A7/ZIP7 promotes intestinal epithelial
self-renewal by resolving ER stress PLOS Genetics 12(10);e1006349 (2016)
15. Wang Q, Yokoo H, Takashina M, Sakata K, Ohashi W, Abdelzaher LA, Imaizumi T,
Sakamoto T, Hattori K, Matsuda N, Hattori Y. Anti-inflammatory profile of levosimendan
in cecal ligation-induced septic mice and in lipopolysaccharide-stimulated macrophage.
Crit. Care Med. 43(11);e508-520 (2015)
16. Ohashi W., Hattori K., Hattori Y. Control of Macrophage Dynamics as a Potential
Therapeutic Approach for Clinical Disorders Involving Chronic Inflammation J. Pharmacol.
Exp. Ther. 354(3);240-250 (2015)
17. Kambara K, Ohashi W, Tomita K, Takashina M, Fujisaka S, Hayashi R, Mori H, Tobe K,
Hattori Y. In vivo depletion of CD206+ M2 macrophages exaggerates lung injury inendotoxemic mice Am. J. Pathol. 185(1);162-171 (2015)
18. Tomita K, Takashina M, Mizuno N, Sakata K, Hattori K, Imura J, Ohashi W, Hattori Y.
Cardiac fibroblasts: contributory role in septic cardiac dysfunction J. Surg. Res.

193(2);874-887 (2014)19. Bin BH, Hojyo S, Hosaka T, Bhin J, Kano H, Miyai T, Ikeda M, Kimura-Someya T, Shirouzu
M, Cho EG, Fukue K, Kambe T, Ohashi W, Kim KH, Seo J, Choi DH, Nam YJ, Hwang D,Fukunaka A, Fujitani Y, Yokoyama S, Superti-Furga A, Ikegawa S, Lee TR, Fukada T.
Molecular pathogenesis of Spondylocheirodysplastic Ehlers-Danlos syndrome caused
by mutation of ZIP13 proteins EMBO Mol. Med. 6(8);1028-1042 (2014)
20. Taguchi K, Sakata K, Ohashi W, Imaizumi T, Imura J, Hattori Y. Tonic inhibition by Gprotein-coupled receptor kinase 2 of Akt/endothelial nitric-oxide syntehase signaling inhuman vascular endothelial cells under conditions of hyper glycemia with high insulinlevels. J. Parmacol. Exp. Ther. 349(2);199-208 (2014)
21. Yamasaki S, Hasegawa A, Hojyo S, Ohashi W, Fukada T, Nishida K, Hirano T. A novelrole of the L-type calcium channel a1D subunit as a gatekeeper for intracellular zincsignaling: Zinc wave. PLoS One 7(6);e39654 (2012)
22. Masanura N, Ohashi W, Tsuge N, Imai S, Ishii-Nakamura A, Hirota H, Nagata T, Kumagai
H. Identification of amino acid residues essential for onion lachrymatory factor synthaseactivity. Biosci. Biotechnol. Biochem. 76(3);447-453 (2012)
23. Bin BH, Fukada T, Hosaka T, Yamasaki S, Ohashi W, Hojyo S, Miyai T, Nishida K,Yokoyama S, Hirano T. Biochemical characterization of human ZIP13 protein: a homodimerized zinc transporter involved in the spondylocheiro dysplastic Ehlers-Danlos
syndrome. J. Biol. Chem. 286(46);40255-40265 (2011)24. Hojyo S, Fukada T, Shimoda S, Ohashi W, Bin BH, Koseki H, Hirano T. The zinctransporter SLC39A14/ZIP14 controls G-protein coupled receptor-mediated signaling
required for systemic growth. PLoS One 6(3);e18059 (2011)
25. Ohashi W, Hirota H, Yamazaki T. Solution structure and fluctuation of the Mg2+-boundform of calmodulin C-terminal domain. Protein Sci. 20(4);690-701 (2011)
26. Kitabayashi C, Fukada T, Kanamoto M, Ohashi W, Hojyo S, Atsumi T, Ueda N, Azuma I,Hirota H, Murakami M, Hirano T. Zinc suppresses Tn17 development via inhibition ofSTAT3 activation. Int. Immunol. 22(5);375-386 (2010)
27. Ohashi W, Inouye S, Yamazaki T, Hirota H. NMR analysis of the Mg2+-binding propertiesof aequorin, a Ca2+-binding photoprotein. J. Biochem. (Tokyo) 138(6);613-620 (2005)
28. Ohashi W, Inouye S, Yamazaki T, Doi-Katayama Y, Yokoyama S, Hirota H. Backbone 1H,13C and 15N resonance assignments for the Mg2+-bound form of the Ca2+-binding
photoprotein aequorin. J. Biomol. NMR 31(4);375-376 (2005)
29. Yano Y, Takemoto T, kobayashi S, Yasui H, Sakurai H, Ohashi W, Niwa M, Futaki S,Sugiura Y, Matsuzaki K. Topological stability and self-association of a completelyhydrophobic model transmembrane helix in lipid bilayers. Biochemistry 41(9);3073-3080(2002)
30. Futaki S, Ohashi W, Suzuki T, Niwa M, Tanaka S, Ueda K, Harashima H, Sugiura Y.
Stearylated arginine-rich peptides: a new class of transfection systems. Bioconjug. Chem.
12(6);1005-1011 (2001)
31. Futaki S, Suzuki T, Ohashi W, Yagami T, Tanaka S, Ueda K, Sugiura Y. Arginine-richpeptides. An abundant source of membrane-permeable peptides having potential as
carriers for intracellular protein delivery. J. Biol. Chem. 276(8);5836-5840 (2001)