Instructions For UseSMS-1-IFU
Rev. Date: April 21, 2016

Revision: 3

Page 1 of 2

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Mucicarmine Stain Kit(Modified Southgates’s)
Description:

Mucicarmine Stain Kit (Modified Southgate’s) is intended for use in the histological visualization of acidmucopolysacharides in tissue sections. This product is useful in distinguishing mucin negative
undifferentiated squamous cell lesions from mucin positive adenocarcinomas. In addition, this productwill stain the mucopolysaccharide capsule of Cryptococcus neoformans.
Mucin:
Capsule of Cryptococcus:
Nuclei:Other tissue components:

Pink/RedRedBlue to Green
Yellow

Uses/Limitations:Not to be taken internally.
For In-Vitro Diagnostic use only.
Histological applications.
Do not use if reagents become cloudy.
Do not use past expiration date.
Use caution when handling reagents.
Non-Sterile.

Control Tissue:

Colon
Intestine
Bronchi Epithelial cells

Ordering information regarding individual components
on back page!
Kit Contents:
Item #HMM125
BRT125
SMG125
TZQ125

Precautions:

Kit Contents
Hematoxylin, Mayer’s
Bluing Reagent
Mucicarmine Solution
Tartrazine Solution

Storage18-25°C
18-25°C
2-8° C
18-25°C

Avoid contact with skin and eyes.
Harmful if swallowed.
Follow all Federal, State, and local regulations regarding disposal.

25° C
Storage: 2° C

Mixed Storage Conditions.
Separate Contents.
Doc: IFU-TemplateMixedStoragerev2

Volume125 ml125 ml125 ml125 ml

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP EmergoEurope (31)(0) 70 345-8570
Molsnstraat 152513 BH Hague, The Netherlands

Instructions For UseSMS-1-IFU
Rev. Date: April 21, 2016

Revision: 3

Page 2 of 2

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Procedure:
1.

Deparaffinize sections if necessary and hydrate to distilled water.

2.

If sections are Zenker-fixed, remove mercuric chloride crystals using iodine and clear with sodium thiosulfate. Rinse inrunning tap water.

3.

Stain slide in Hematoxylin for 2-3 minutes.

4.

Rinse for 2 minutes in running tap water.

5.

Dip slide in Bluing Reagent for 30 seconds.

6.

Rinse slide in distilled water.

7.

Stain slide in Mucicarmine Solution for 10 minutes.

8.

Rinse quickly in tap water followed by a quick rinse in distilled water.

9.

Stain slide in Tartrazine Solution for 1 minute.

10.

Rinse slide 1 time in absolute alcohol.

11.

Dehydrate slide quickly in 3 changes of absolute alcohol.

12.

Clear in 2 changes of xylene or xylene substitute, and mount in synthetic resin.

References:1.

Sheenan, D.C., Hrapchak, B.B. Theory and Practice of Histotechnology, 2nd Edition. Battelle Press, Columbus, OH. Pages 172-173.

Bulk Reagent Ordering Information and Current Pricing at www.scytek.com
Description:

Catalog #Volume

Hematoxylin, Mayer’s(Lillie’s Modification)

HMM500
HMM999
HMM3800

500 ml1000 ml
1 Gallon

Bluing Reagent

BRT500
BRT999
BRT3800

500 ml10000 ml
1 Gallon

Mucicarmine Solution(Southgate’s)

SMG125
SMG500
SMG999125 ml500 ml1000 ml

Tartrazine Solution

TZQ125
TZQ500
TZQ999125 ml500 ml1000 ml

25° C
Storage: 2° C

Mixed Storage Conditions.
Separate Contents.
Doc: IFU-TemplateMixedStoragerev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CVP EmergoEurope (31)(0) 70 345-8570
Molsnstraat 152513 BH Hague, The Netherlands