Instructions For UseIRN-1-IFU
Rev. Date: Feb. 11, 2012

Revision: 3

Page 1 of 2

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Iron Stain Kit
Description:

The Iron Stain Kit is intended for use in the detection of ferric iron in tissues, blood smears, or bonemarrow smears. Ferric iron is normally found in small amounts in bone marrow and the spleen.
Abnormally large deposits may be seen in hemochromatosis and hemosiderosis. This product is basedon the Prussian Blue reaction in which ionic iron reacts with acid ferrocyanide producing a blue color.
Tissue Sections
Iron:
Nuclei:Background:
Bone or Blood Smears
Sideroblasts:

Bright BlueRedPink
These are nucleated erythrocytes containing at least one small blue granule.
If the blue granules surround the nucleus, the cell is a ringed sideroblast.

Siderocytes:

These are non-nucleated erythrocytes containing at least one blue granule.

Reticuloendothelial Iron:

Usually seen as blue particles on the marrow smear or as blue particles in thecytoplasm or phagocytic cells.

Uses/Limitations:Not to be taken internally.
For In-Vitro Diagnostic use only.
Histological applications.
Do not use if reagents become cloudy.
Do not use past expiration date.
Use caution when handling reagents.
Non-Sterile.

Control Tissue:

SpleenBone Marrow

Ordering information regarding individual components
on back page!
Kit Contents:
Item #PFB500
HQB500
NFS125

Precautions:

Kit Contents
Potassium Ferrocyanide Solution
Hydrochloric Acid Solution (2%)
Nuclear Fast Red Solution

Doc: IFU-Template18-25rev2

Storage18-25°C
18-25°C
18-25°C

Avoid contact with skin and eyes.
Harmful if swallowed.
Follow all Federal, State, and local regulations regarding disposal.
25° C

Storage: 18° C

Volume500 ml
500 ml125 ml

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CV
P EmergoEurope (31)(0) 70 345-8570
Molsnstraat 152513 BH Hague, The Netherlands

Instructions For Use

IRN-1-IFU
Rev. Date: Feb. 11, 2012

Revision: 3

Page 2 of 2

P.O. Box 3286 - Logan, Utah 84323, U.S.A. - Tel. (800) 729-8350 – Tel. (435) 755-9848 - Fax (435) 755-0015 - www.scytek.com

Procedure:
Note:

Use acid-washed or bleach-washed glassware.
Rinse all glassware with distilled water prior to use.
Do not use metal forceps to transfer slide during staining procedure.

1.

Deparaffinize sections if necessary and hydrate to distilled water.

2.

Mix equal volumes of Potassium Ferrocyanide Solution and Hydrochloric Acid Solution to make a working Iron Stain
Solution. Use once and discard.

3.

Incubate slide in working Iron Stain Solution for 3-5 minutes.

4.

Rinse thoroughly in distilled water.

5.

Stain slide in Nuclear Fast Red Solution for 5 minutes.

6.

Rinse in 4 changes of distilled water.

7.

Dehydrate in 3 changes of absolute alcohol for 2 minutes each.

8.

Clear, and mount in synthetic resin.

References:1.

Sheenan, D.C., Hrapchak, B.B. Theory and Practice of Histotechnology, 2nd Edition. Battelle Press, Columbus, OH. Page 217. 1980

2.

Carson, F.L., Histotechnology; A Self-Instructional Text, ASCP Press, Chicago, IL. Pages 214-215. 1990

Bulk Reagent Ordering Information and Current Pricing at www.scytek.com
Description:

Catalog #Volume

Potassium Ferrocyanide Solution

PFB500
PFB999500 ml1000 mlHydrochloric Acid Solution (2%)

HQB500
HQB999500 ml1000 ml

Nuclear Fast Red Solution(Enhanced Stability)

NFS125
NFS500
NFS999125 ml500 ml1000 ml

25° C
Storage: 18° C

Doc: IFU-Template18-25rev2

ScyTek Laboratories, Inc.
205 South 600 West
Logan, UT 84321
U.S.A.

CV
P EmergoEurope (31)(0) 70 345-8570
Molsnstraat 152513 BH Hague, The Netherlands