Surveyor®
® Mutattion Deetectionn Kit forr Standaard Gel Electro
ophoressis
Surveeyor Mutatioon Detectionn Kits provide a simple,
accurrate and cost‐effective means to scan DNADfragmen
ntsfor mmutations withh unmatched sensitivity
sandd specificity.

Soomatic, germ‐lline and mitocchondrial muttations
utions and SN
NPs Low‐copy
Inssertions, deletions, substitu
muutations and ppooled DNA samples

Compplete Mutatioon Detection System: Con
ntains Surveyyor
Nucleease, all reageents and plasm
mid controls.

MMutation Deetection in FFour Easy SttepsAmplifyy Mutant and R
Reference DNAA

Location of misma
atch
5’

3’

3’5’
SURVEYOR NuucleaseHybridize DNA
A Hetero- and H
Homoduplex FFormation

5’

3’

3’

5’
Resulting fragmments
3’Benefits

5’

Deetermines mu
utation loci Saaves
tim
me and moneyy

5500 bp
3300 bp
2200 bp1100 bp

DNA Marker#13

poost‐PCR or posst‐reaction cleean‐up Highth roughput sen
nsitivity and sp
pecificity#3

Figurre 1. Agarose (2.5% w/v) gel elecctrophoresis offragmments generated by digestion of 16
60 ng of DNNAwith 1 μl of Survveyor Nucleaase S at 42 °C° for 20 min.
Analyysis of 75% of the digeested DNA wasw performeed.
The 584‐bp amplicons werew
PCR amplifiedawiithOptimmase® Polyme
erase from oneo wild‐typee reference andthreee mutant genes. Samples#26, #3 and #13 hadh one (T>C; fragments werew
242 + 3442bp loong), two (A>GG and T>C; fraagments weree 135+ 44
49 bp and 220
2+ 364 bpb long) andd three (singlle‐base deletion, T>>C and C>A; fragments werew
120 + 46
64bp, 170 + 414 bp
b and 226 + 358 bp loong) mutations
respeectively. A 100‐bp ladd
der was runn as a sizing
standdard. The ge
el was staine
ed with ethidium bromidde,visuaalized
withhaUVVtransillum
minatoranndphottographed.

– Streamlines D
DNA sequencinng– Decreases sequencing backlog and analysiss time
– Validates sequencing resultss and analysis– Decreases chromatogram annalysis by 90% No#26Surveeyor Nucleasee is a mism
match‐specific endonucleasethat recognizes mismatches
min heteroduplexed DNA anndcleavves both straands on the 3’ side of the mismatchproviding specificc informatioon on mutaation locatio
on,ntation and type.
orien

Homoduplex

3’

Diirect Fragmentt Analysis

DNA Marker5’Ad
dd SURVEYOR
R NucleaseSurveyor Mutation Detection Kit Components – All Optimized to Work Together• Surveyor Nuclease S• Surveyor Nuclease Enhancer S• Stop Solution• Control Plasmids and Primers• 0.15M Mg2Cl Solution

ProductsSurveyor Mutation Detection Kit for Standard Gel Electrophoresis
Quantity
25 reactions
100 reactions
1,000 reactions

Catalog No.
706025706020
706021

Shipping and Storage
Surveyor products are shipped frozen. Store product at ‐20 ºC in a non‐frost‐free freezer. Enzyme is guaranteed for a period of 6months if stored as directed.

Quality Control
Every component is quality control tested as indicated on the certificate of analysis, included in each kit.

Selected References
1. DHPLC/Surveyor Nuclease: A Sensitive, Rapid and Affordable Method to Analyze BRCA1 and BRCA2 Mutations in Breast Cancer Families. Pilato, B. et al. Mol.
Biotechnol. 52, 8‐15. (2011).
2. A molecular analysis of mutations at the complex dumpy locus in Drosophila melanogaster. Carmon, A. et al. PLoS ONE 5, e12319. (2010).
3. Screening for mutations in kidney‐related genes using Surveyor nuclease for cleavage at heteroduplex mismatches. Voskarides, K. & Deltas, C. J. Mol. Diagn. 11,311‐318. (2009).
4. Novel method for genomic analysis of PKD1 and PKD2 mutations in autosomal dominant polycystic kidney disease. Tan, Y. et al. Hum. Mutat. 30, 264‐272.
(2009).
5. Single nucleotide polymorphisms, apoptosis, and the development of severe late adverse effects after radiotherapy. Azria, D. et al. Clin. Cancer Res. 14, 6284‐6288. (2008).
6. Rapid identification of unknown heteroplasmic mutations across the entire human mitochondrial genome with mismatch‐specific Surveyor Nuclease Bannwarth,
S. et al. Nat. Protoc. 1, 2037‐2047. (2006).
7. Mitochondrial DNA damage and repair in RPE associated with aging and age‐related macular degeneration. Lin, H. et al. Invest. Ophthalmol. Vis. Sci.
52, 3521‐3529. (2011).
8. Allelic dilution obscures detection of a biologically significant resistance mutation in EGFR ‐amplified lung cancer. Engelman, J.A. et al. J. Clin. Invest.
116, 2695‐2706. (2006).
9. A rapid and sensitive enzymatic method for epidermal growth factor receptor mutation screening. Jänne, P.A. et al. Clin. Cancer Res. 12, 751‐758. (2006).
10. Temperature‐mediated heteroduplex analysis for the detection of drug‐resistant gene mutations in clinical isolates of Mycobacterium tuberculosis bydenaturing HPLC, Surveyor nuclease. Shi, R. et al. Microbes Infect. 8, 128‐135. (2006).

www.IDTDNA.com/Surveyor
For questions or additional assistance, please contact IDT Customer Care at:
USA & Canada
custcare@idtdna.com
1–800–328–2661

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1800 092 259

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For specific trademark and licensing information, see www.idtdna.com/trademarks.
Surveyor products are covered by multiple IDT patents and are sold by IDT under exclusive license to U.S. Patents 6,391,557, 5,869,245. This product is sold for non‐commercial, research use only. Any non‐clinical, commercial use, including without limitation the use of any Surveyor product in the manufacture of nucleic acid products ofany type, requires a separate license from IDT. Any clinical use of any Surveyor product requires a separate license from Transgenomic, the exclusive licensee of Surveyor
products in the clinical and diagnostic fields. Surveyor is a registered trademark of IDT.