Curriculum Vitae

Hideyuki Takeuchi, Ph.D.
Professor
Department of Biochemistry
University of Shizuoka School of Pharmaceutical Sciences52-1 Yada, Suruga, Shizuoka, Shizuoka 422-8526 Japan

Phone: +81-54-264-5725 (Office)Phone: +81-54-264-5720 (Lab)FAX: +81-54-264-5723
Email: htakeuchi@u-shizuoka-ken.ac.jp

EDUCATION
1996-2001
1992-1996

The University of Tokyo, Tokyo, Japan
Graduate School of Pharmaceutical Sciences
Ph.D. in Pharmacology, March 2001
The University of Tokyo, Tokyo, Japan
B.S. in Pharmacology, March 1996

PROFESSIONAL EXPERIENCE
2021.4-present
2017.4-2021.3
2015.7-2017.3
2008.4-2015.6
2006.4-2008.3
2003.4-2009.3
2002.4-2003.3
2000.1-2002.3

Professor, Department of Biochemistry,
University of Shizuoka School of Pharmaceutical Sciences. Shizuoka, Japan.
Associate Professor, Department of Molecular Biochemistry,
Nagoya University Graduate School of Medicine. Nagoya, Japan.
Associate Research Scientist, Complex Carbohydrate Research Center,
The University of Georgia. Athens, Georgia
Research Assistant Professor, Department of Biochemistry and Cell Biology,
Stony Brook University. Stony Brook, New York
Research Scientist, Department of Biochemistry and Cell Biology,
Stony Brook University. Stony Brook, New York
Assistant Professor, Graduate School of Pharmaceutical Sciences,
The University of Tokyo. Tokyo, Japan (Adjunct 2006.4-2009.3)
Postdoctoral Fellow, Graduate School of Pharmaceutical Sciences,
The University of Tokyo. Tokyo, Japan
Research Fellow of the Japan Society for the Promotion of Science,
Graduate School of Pharmaceutical Sciences,
The University of Tokyo. Tokyo, Japan

HONORS & AWARDS
2018
2011
2006
2000-2002
1998-1999

Daiko Foundation research grant for international academic exchange
Gordon Research Conferences (Glycobiology) achievement award
The Astellas Foundation fellowship for research on metabolic disorders
Grant-in-Aid for JSPS Fellows
The Japan Scholarship Foundation Scholarship for Graduate Students

Hideyuki Takeuchi, Ph.D.

Page-1FUNDING
2022-2023
2022-2023
2019-20222019
2019-2021
2019-2022
2019-202220182018
2017-201920172017
2009-2011

Research grant from Mizutani Foundation for Glycoscience (PI: Takeuchi. 3,000,000 yen)
Research grant from The Uehara Memorial Foundation (PI: Takeuchi. 5,000,000 yen)
JSPS KAKENHI Grant-in-Aid for Fostering Joint International Research (B) JP19KK0195(PI: Takeuchi. 14,200,000 yen)
Research grant from The Kato Memorial Trust for Nambyo Research (PI: Takeuchi.
2,000,000 yen)
JSPS KAKENHI Grant-in-Aid for Exploratory Research JP19K22490 (PI: Takeuchi.
5,000,000 yen)
JSPS KAKENHI Grant-in-Aid for Scientific Research (B) JP19H03176 (PI: Takeuchi.
13,400,000 yen)
JSPS KAKENHI Grant-in-Aid for Scientific Research (B) JP19H03416 (PI: Tetsuya Okajima,co-I: Takeuchi. 1,350,000 yen)
Research grant from Aichi Cancer Research Foundation (PI: Takeuchi. 500,000 yen)
Research grant from Kitamura Memorial Fund (PI: Takeuchi. 800,000 yen)
JSPS KAKENHI Grant-in-Aid for Research Activity Start-up JP17H06743 (PI: Takeuchi.
2,100,000 yen)
Research grant from Takeda Science Foundation (PI: Takeuchi. 2,000,000 yen)
Research grant from Daiichi Sankyo Foundation of Life Science (PI: Takeuchi. 2,000,000
yen)Research grant from Mizutani Foundation for Glycoscience (PI: Takeuchi. $ 50,000)

EXPERIENCE OF TEACHING
University of Shizuoka•
Biological chemistry for the second-year undergraduate students•
Glycobiology for the third-year undergraduate students•
Biochemistry for graduate students•
Glycoscience for graduate students•
Biochemistry laboratory course for undergraduate students•
Biochemistry laboratory course for graduate students
Nagoya University•
Basic seminar for the first-year undergraduate students•
Cancer Biology for the second-year undergraduate students•
Biochemistry laboratory course for the third-year undergraduate students•
Basic medical seminar for the third-year undergraduate students
The University of Tokyo•
Introduction to Life Sciences for the graduate students•
Basic Bioscience for the third-year undergraduate students•
Biochemistry laboratory course for the third-year undergraduate students

Hideyuki Takeuchi, Ph.D.

Page-2

EXPERIENCE OF TRAINING STUDENTS
Graduate Students
Yusuke Urata (2020, Ph.D.)
Weiwei Wang
Yohei Tsukamoto
Yukihiro Mita
Chenyu Ma
Katsuaki Usami (2009, Ph.D.)
Kentaro Kato (2004, Ph.D.)
Akira Kanoh (2003, Ph.D.)

Project
Examining roles of XXYLT1 in squamous cell carcinoma
Examining roles of Notch O-glycosylation in T-ALL
Mass spectrometric analysis of Notch O-glycosylation
Examining roles of GXYLT1/2 in Notch signaling
Identification of inhibitors of POGLUT1
Examining functions of macrophage C-type lectin in Ebola virus infection
Biochemical analysis of polypeptide GalNAc-transferases
Examining functions of intestinal mucins in food allergy

Rotation students
Huilin Hao
Daniel Williamson
Natasha Perumal
Rachel K. Lopilato
Steven J. Berardinelli
Ao Zhang
Kira Schultheiss
Catherine Peters
Dong-Hyuk Ki

Project
Establishing POFUT1&POGLUT1 knockout cells by CRISPR/Cas9
technology
Establishing new POGLUT2/3 knockout cells by CRISPR/Cas9 technology
Establishing POFUT1&2 knockout cells by CRISPR/Cas9 technology
Establishing B3GLCT knockout cells by CRISPR/Cas9 technology
Functional analysis of B3GLCT in mammalian Knockout cells
Establishing POFUT1&2 knockout cells by CRISPR/Cas9 technology
Exploring UDP-xylose: glucoside a3-xylosyltransferases
Exploring UDP-xylose: glucoside a3-xylosyltransferases
Examining effect of O-glycosylation on Notch ligand binding

Undergraduate students
Atsuki Uchida
Yuka Sakuma
Hiroaki Sago
Wataru Saiki (2021, B.S.)
Joshua Kantharia (2014, B.S.)
Hillary Moss (2012, B.S.)
Ahmed Rab (2012, B.S.)

Project
Examining xylosyl-extension of O-glucose glycans in Notch signaling
Examining the effect of O-glucose glycans on Notch-ligand interaction
Examining roles of GXYLT1/2 in AML
Examining xylosyl-extension of O-glucose glycans in Notch signaling
Examining regulation of Notch-ligand interaction by glycosylation
Examining fringe specificity acting toward Drosophila Notch EGF repeats
Examining elongation of O-glucose glycans on Drosophila Notch

Hideyuki Takeuchi, Ph.D.

Page-3

PUBLICATIONS
1.

Takahashi T, Kurebayashi Y, Tani K, Yamazaki M, Minami A, and Takeuchi H.
catechins on mumps virus infection.
J Funct. Foods 2021; 87: 104817.

The antiviral effect of2.

Piniello B, Lira-Navarrete E, Takeuchi H, Takeuchi M, Haltiwanger RS, Hurtado-Guerrero R, and Rovira
C. Asparagine tautomerization in glycosyltransferase catalysis. The molecular mechanism of protein Ofucosyltransferase 1.
ACS Catal 2021; 11: 9926-9932.

3.

Barua R, Mizuno K, Tashima Y, Ogawa M, Takeuchi H, Taguchi A, and Okajima T. Bioinformatics andfunctional analyses implicate potential roles for EOGT and L-fringe in pancreatic cancers.
Molecules 2021 Feb 7; 26 (4): 882.

4.

Hashiguchi H, Tsukamoto Y, Ogawa M, Tashima Y, Takeuchi H, Nakamura M, Kawashima H, FujishiroM, and Okajima T. Glycoproteomic analysis identifies cryptdin-related sequence 1 as O-glycosylated
protein modified with a1,2-fucose in the small intestine.
Arch Biochem Biophys 2020 Nov 30; 695: 108653.

5.

Ma C, Takeuchi H, Hao H, Yonekawa C, Nakajima K, Nagae M, Okajima T, Haltiwanger RS, and Kizuka
Y. Differential labeling of glycoproteins with alkynyl fucose analogs.
Int J Mol Sci 2020 Aug 20; 21 (17): E6007.

6.

Urata Y, Saiki W, Tsukamoto Y, Sago H, Hibi H, Okajima T, and Takeuchi H. Xylosyl extension of Oglucose glycans on the extracellular domain of NOTCH1 and NOTCH2 regulates Notch cell surface
trafficking.
Cells 2020 May 14; 9 (5): E1220.

7.

Alam SMD, Tsukamoto Y, Ogawa M, Senoo Y, Ikeda K, Tashima Y, Takeuchi H, and Okajima T. NGlycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral
endoplasmic reticulum localization.
J Biol Chem 2020 May 6; 295 (25): 8560-74.

8.

Ogawa M*, Tashima Y*, Sakaguchi Y, Takeuchi H, and Okajima T. Contribution of extracellular OGlcNAc to the stability of folded epidermal growth factor-like domains and Notch1 trafficking.
Biochem Biophys Res Commun 2020 May 21; 526 (1): 184-190. (*: Equal contribution)

9.

Servián-Morilla E, Cabrera-Serrano M, Johnson K, Pandey A, Ito A, Rivas E, Chamova T, Muelas N,
Mongini T, Nafissi S, Claeys KG, Grewal RP, Takeuchi M, Hao H, Bönnemann C, Lopes Abath Neto O,
Medne L, Brandsema J, Töpf A, Taneva A, Vilchez JJ, Tournev I, Haltiwanger RS, Takeuchi H, Jafar-Nejad
H, Straub V, and Paradas C. POGLUT1 biallelic mutations cause myopathy with reduced satellite cells, adystroglycan hypoglycosylation and a distinctive radiological pattern.
Acta Neuropathol 2020 Mar; 139 (3): 565-82.

10. Ralser DJ, Takeuchi H, Fritz G, Basmanav FB, Effern M, Sivalingam S, El-Shabrawi-Caelen L,
Hideyuki Takeuchi, Ph.D.

Page-4

Degirmentepe EN, Kocatürk E, Singh M, Booken N, Spierings NMK, Schnabel V, Heineke A, Knuever J,
Wolf S, Wehner M, Tronnier M, Leverkus M, Tantcheva-Poór I, Wenzel J, Oji V, Has C, Hölzel M, FrankJ, Haltiwanger RS, and Betz RC. Altered Notch signaling in Dowling-Degos disease: Additional
mutations in POGLUT1 and further insights into disease pathogenesis.
J Invest Dermatol 2019 139: 960-964.
11.

Takeuchi H*, Schneider M*, Williamson DB, Ito A, Takeuchi M, Handford PA, and Haltiwanger RS. Twonovel protein O-glucosyltransferases that modify sites distinct from POGLUT1 and affect Notch trafficking
and signaling.
Proc Natl Acad Sci USA 2018 Sep 4; 115 (36): E8395-402. (*: Equal contribution)

12.

Ogawa M, Senoo U, Ikeda K, Takeuchi H, and Okajima T. Structural divergence in O-GlcNAc glycans
on epidermal growth factor-like repeats of mammalian Notch1.
Molecules 2018 Jul; 23 (7): E1745.

13.

Takeuchi H, Wong D, Schneider M, Freeze HH, Takeuchi M, Berardinelli SJ, Ito A, Lee H, Nelson SF, andHaltiwanger RS. Variant in human POFUT1 reduces enzymatic activity and likely causes a recessive
microcephaly, global developmental delay with cardiac and vascular features.
Glycobiology 2018 May; 28 (5): 276-83. (Recommended by Faculty of 1000 Prime)

14.

Fujihira H, Usami K, Matsuno K, Takeuchi H, Denda-Nagai K, Furukawa JI, Shinohara Y, Takada A,
Kawaoka Y, and Irimura T. A critical domain of Ebolavirus envelope glycoprotein determines glycoform
and infectivity.
Sci Rep 2018 Apr; 8 (1): 5495.

15.

Schneider M, Kumar V, Nordstrom LU, Feng L, Takeuchi H, Hao H, Luca VC, Garcia KC, Stanley P, Wu
P, and Haltiwanger RS. Inhibition of Delta-induced Notch signaling using fucose analogs.
Nature Chem Biol 2018 Jan; 14 (1): 65-71.

16.

Weh E, Takeuchi H, Muheisen S, Haltiwanger RS, and Semina EV. Functional characterization ofzebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gen mutated in Peters Plus syndrome.
Plos One 2017 Sep; 12 (9): e0184903.

17.

Takeuchi H*, Yu H*, Hao H, Takeuchi M, Ito A, Li H, and Haltiwanger RS. O-Glycosylation modulates
the stability of epidermal growth factor-like repeats and thereby regulates Notch trafficking.
J Biol Chem 2017 Sep; 292 (38): 15964-73. (*: Equal contribution)

18.

Sheikh MO, Halmo SM, Patel S, Middleton D, Takeuchi H, Schafer CM, West CM, Haltiwanger RS, Avci
FY, Moremen KW, and Wells L. Rapid screening of sugar-nucleotide donor specificities of putative
glycosyltransferases.
Glycobiology 2017 Mar; 27 (3): 206-12.

19.

Hubmacher D, Schneider M, Berardinelli SJ, Takeuchi H, Willard B, Reinhardt DP, Haltiwanger RS, andApte SS.
Unusual life cycle and impact on microfibril assembly of ADAMTS17, a secreted
metalloprotease mutated in genetic eye disease.
Sci Rep 2017 Feb; 7: 41871.

Hideyuki Takeuchi, Ph.D.

Page-520.

Servián-Morilla E*, Takeuchi H*, Lee TV*, Clarimon J, Mavillard F, Area-Gómez E, Rivas E, NietoGonzález JL, Rivero MC, Cabrera-Serrano M, Gómez-Sánchez L, Martínez-López JA, Estrada B, Márquez
C, Morgado Y, Suárez-Calvet X, Pita G, Bigot A, Gallardo E, Fernández-Chacón R, Hirano M, Haltiwanger
RS, Jafar-Nejad H, Paradas C. A POGLUT1 mutation causes a muscular dystrophy with reduced Notch
signaling and satellite cell loss.
EMBO Mol Med 2016 Nov; 8 (11): 1289-1309. (*: Equal contribution)21.

Yu H*, Takeuchi H*, Takeuchi M, Kantharia J, Haltiwanger RS#, and Li H#. Structural analysis of Notchregulating Rumi reveals basis for pathogenic mutations.
Nature Chem Biol 2016 Sep; 12 (9): 735-40. (*: Equal contribution, #: Co-corresponding author)

22.

Benz BA, Nandadasa S, Takeuchi M, Grady RC, Takeuchi H, LoPilato RK, Kakuda S, Somerville RPT,
Apte SS, Haltiwanger RS#, and Holdener BC#. Genetic and biochemical evidence that gastrulation defects
in Pofut2 mutants result from defects in ADAMTS9 secretion.
Dev Biol 2016 Aug; 416 (1): 111-22. (#: Co-corresponding author)

23.

Valero-Gonzalez J*, Leonhard-Melief C*, Lira-Navarrete E, Jimenez-Oses G, Hernandez-Ruiz C, Pallares
CP, Yruela I, Vasudevan D, Lostao A, Corzana F, Takeuchi H, Haltiwanger RS, and Hurtado-Gerrero R.
A proactive role of water molecules in acceptor recognition by Protein-O-fucosyltransferase 2.
Nature Chem Biol 2016 Apr; 12 (4): 240-6. (*: Equal contribution)

24.

Yu H, Takeuchi M, Kantharia J, LeBarron J, London E, Bakker H, Haltiwanger RS, Li H#, and Takeuchi
H#. Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism.
Nature Chem Biol 2015 Nov; 11 (11): 847-54. (#: Co-corresponding author)

25. Vasudevan D, Takeuchi H, Johar SS, Majerus E, and Haltiwanger RS.
Peters plus syndrome mutations disrupt a non-canonical ER quality control mechanism.
Curr Biol 2015 Feb; 25 (3): 286-95.
26. Taylor P*, Takeuchi H*, Sheppard D*, Chandramouli C, Lea SM, Haltiwanger RS, and Handford PA.
Fringe-mediated extension of O-linked fucose in the ligand-binding region of Notch1 increases binding to
mammalian Notch ligands.
Proc Natl Acad Sci USA 2014 May; 111 (20): 7290-5. (*: Equal contribution)
27. Takeuchi H and Haltiwanger RS. Enzymatic analysis of the protein O-glycosyltransferase, Rumi, acting
toward epidermal growth factor-like (EGF) repeats.
Methods Mol Biol 2013; 1022: 119-28.
28.

Takeuchi H, Kantharia J, Sethi, MK, Bakker H, and Haltiwanger RS. Site-specific O-glucosylation of theepidermal growth factor-like (EGF) repeats of Notch: efficiency of glycosylation is affected by proper
folding and amino acid sequence of individual EGF repeats.
J Biol Chem 2012 Oct; 287 (41): 33934-44.

29. Tamada Y, Takeuchi H, Suzuki N, Aoki D, and Irimura T. Cell surface expression of hyaluronan on
human ovarian cancer cells inversely correlates with their adhesion to peritoneal mesothelial cells.

Hideyuki Takeuchi, Ph.D.

Page-6

Tumor Biol30.

2012 Aug; 33 (4): 1215-22.

Sethi, MK, Buettner FFR, Ashikov A, Krylov VB, Takeuchi H, Nifantiev NE, Haltiwanger RS, GerardySchahn R, and Bakker H. Moleculer cloning of a xylosyltransferase that transfers the second xylose to Oglucosylated epidermal growth factor repeats of Notch.
J Biol Chem 2012 Jan; 287 (4): 2739-48.

31. Takeuchi H, Fernández-Valdivia RC, Caswell DS, Nita-Lazar A, Rana NA, Macnaughtan MA, Jafar-Nejad
H, and Haltiwanger RS. Rumi functions as both a protein O-glucosyltransferase and a protein Oxylosyltransferase.
Proc Natl Acad Sci USA 2011 Oct; 108 (40): 16600-5.
32. Rana NA*, Nita-Lazar A*, Takeuchi H, Kakuda S, Luther KB, and Haltiwanger RS.
trisaccharide is present at high but variable stoichiometry at multiple sites on mouse Notch1.
J Biol Chem 2011 Sep; 286 (36): 31623-37. (*: Equal contribution)

O-Glucose

33. Fernández-Valdivia RC, Takeuchi H, Samarghandi A, Lopez M, Leonardi J, Haltiwanger RS, and JafarNejad H. Regulation of mammalian Notch signaling and embryonic development by the protein Oglucosyltransferase Rumi.
Development 2011 May; 138 (10): 1925-34.
34. Du J, Takeuchi H, Leonhard-Melief C, Shroyer KR, Dlugosz M, Haltiwanger RS, and Holdener BC. Ofucosylation of thrombospondin type 1 repeats restricts epithelial to mesenchymal transition (EMT) andmaintains epiblast pluripotency during mouse gastrulation.
Dev Biol 2010 Oct; 346 (1): 25-38.
35. Kato K, Takeuchi H, Kanoh A, Miyahara N, Nemoto-Sasaki Y, Morimoto-Tomita M, Matsubara A, Ohashi
Y, Waki M, Usami K, Mandel U, Clausen H, Higashi N, and Irimura T. Loss of UDP-GAlNAc:
polypeptide N-acetylgalactosaminyltransferase 3 and reduce O-glycosylation in colon carcinoma cells
selected for hepatic metastasis.
Glycoconj J 2010 Feb; 27 (2): 267-76.
36. Sethi MK, Buettner FF, Krylov VB, Takeuchi H, Nifantiev NE, Haltiwanger RS, Gerardy-Schahn R, andBakker H. Identification of glycosyltransferase 8 family members as xylosyltransferases acting on Oglucosylated notch epidermal growth factor repeats.
J Biol Chem 2010 Jan; 285 (3): 1582-6.
37. Fang J, Izawa R, Gomez-Santos L, Ueno S, Sawaguchi T, Usami K, Nodera Y, Takeuchi H, Ohashi Y,
Higashi N, and Irimura T. Potentiation of proliferation of some but not all human colon carcinoma celllines by immobilized hepatic asialoglycoprotein receptor 1.
Oncology Res 2009; 17 (10): 437-45.
38. Ishikawa HO, Takeuchi H, Haltiwanger RS, and Irvine KD. Four-jointed is a Golgi kinase that
phosphorylates a subset of cadherin domains.
Science 2008 Jul; 321 (5887): 401-4. (Recommended by Faculty of 1000)

Hideyuki Takeuchi, Ph.D.

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39. Kato K, Takeuchi H, Ohki T, Waki M, Usami K, Hassan H, Clausen H, and Irimura T.
differential arrangements of O-glycans on mucin repeats.
Biochem Biophys Res Commun 2008 Jul; 371 (4): 698-701.

A lectin recognizes

40. Acar M*, Jafar-Nejad H*, Takeuchi H*, Rajan A, Ibrani D, Rana NA, Pan H, Haltiwanger RS, and Bellen
H. Rumi, a CAP10 domain protein, is a glycosyltransferase that modifies Notch and is required for Notch
signaling.
Cell 2008 Jan; 132 (2): 247-58. (*: Equal contribution, Previewed in Cell 2008 132(2): 177-9,
Highlighted in Functional Glycomics (14 Feb 2008), Recommended by Faculty of 1000)
41. Kanoh A*, Takeuchi H*, Kato K, Waki M, Usami K, and Irimura T. Interleukin-4 induces specific ppGalNAc-T expression and alterations in mucin O-glycosylation in colonic epithelial cells.
Biochim Biophys Acta 2008 Mar; 1780 (3): 577-584. (*: Equal contribution)
42. Xu A, Haines N, Dlugosz M, Rana NA, Takeuchi H, Haltiwanger RS, and Irvine KD.
reconstitution of the modulation of Drosophila notch-ligand binding by fringe.
J Biol Chem 2007 Nov; 282 (48): 35153-62.

In vitro

43. Tamada Y, Takeuchi H, Suzuki N, Susumu N, Aoki D, and Irimura T. Biological and therapeuticsignificance of MUC1 with sialoglycans in clear cell adenocarcinoma of the ovary.
Cancer Science 2007 Oct; 98 (10): 1586-91.
44. Wandall H, Irazoqui F, Tarp MA, Bennett EP, Mandel U, Takeuchi H, Kato K, Irimura T, Suryanarayanan
G, Hollingsworth MA, and Clausen H. The lectin domains of polypeptide GalNAc-transferases exhibit
carbohydrate-binding specificity for GalNAc: lectin binding to GalNAc-glycopeptide substrates is requiredfor high density GalNAc-O-glycosylation.
Glycobiology 2007 Apr; 17 (4): 374-87.
45. Takeuchi H, Kato K, Hassan H, Clausen H, and Irimura T.
acceptor site of three consecutive threonines.
Eur J Biochem 2002 Dec; 269 (24): 6173-83.

O-GalNAc incorporation into a cluster

46. Takeuchi H, Kato K, Denda-Nagai K, Hanisch FG, Clausen H, and Irimura T. The epitope recognized by
the unique anti-MUC1 monoclonal antibody MY.1E12 involves sialyl alpha 2-3galactosyl beta 1-3Nacetylgalactosaminide linked to a distinct threonine residue in the MUC1 tandem repeat.
J Immunol Methods 2002 Dec; 270 (2): 199-209.
47. Kato K, Takeuchi H, Kanoh A, Mandel U, Hassan H, Clausen H, and Irimura T. N-acetylgalactosamine
incorporation into a peptide containing consecutive threonine residues by UDP-N-acetyl-Dgalactosaminide: polypeptide N-acetylgalactosaminyltransferases.
Glycobiology 2001 Oct; 11 (10): 821-9.
48. Kato K, Takeuchi H, Miyahara N, Kanoh A, Hassan H, Clausen H, and Irimura T. Distinct orders of
GalNAc incorporation into a peptide with consecutive threonines.
Biochem Biophys Res Commun 2001 Sep; 287 (1): 110-5.

Hideyuki Takeuchi, Ph.D.

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49. Iida S, Takeuchi H, Kato K, Yamamoto K, and Irimura T. Order and maximum incorporation of N-acetylD-galactosamine into threonine residues of MUC2 core peptide with microsome fraction of human-coloncarcinoma LS174T cells.
Biochem J 2000 Apr; 347 (Pt 2): 535-42.
50. Iida S, Takeuchi H, Hassan, H, Clausen, H, and Irimura T. Incorporation of N-acetylgalactosamine into
consecutive threonine residues in MUC2 tandem repeat by recombinant human N-acetyl-D-galactosaminetransferase-T1, T2 and T3.
FEBS Lett 1999 Apr; 449 (2-3): 230-4.
CHAPTERS AND REVIEW ARTICLES
1.

Tsukamoto Y and Takeuchi H. Other types of Glycosylation.
Adv Exp Med Biol 2021; 1325: 117-135.

2.

Saiki W, Ma C, Okajima T, and Takeuchi H. Current views on the roles of O-glycosylation in controlling
Notch-ligand interactions.
Biomolecules 2021 Jan; 11 (2): 309.

3.

Urata Y and Takeuchi H. Effects of Notch glycosylation on health and diseases.
Dev Growth Differ 2020 Jan; 62 (1): 35-48.

4.

Takeuchi H. Notch-Modifying Protein O-Glucosyltransferase 1 (POGLUT1): Specificities, Structures,and Human Disease Implications.
Trends in Glycoscience and Glycotechnology 2019; 31: E49-E52.

5.

Yu H and Takeuchi H. Protein O-glucosylation: another essential role of glucose in biology.
Curr Opin Struct Biol 2019 Jan; 56: 64-71.

6.

Takeuchi H. Molecular mechanisms of Notch receptor activation by O-glycosylation.
Farumashia 2018 Oct; 54 (10): 948-952.

7.

Takeuchi H. Biochemical significance of regulation of protein stability by O-glucose glycans.
Seikagaku 2018 Aug; 90 (4): 519-523.

8.

Takeuchi H. Significance of O-glycosylation in Notch signaling.
Seikagaku 2015 Aug; 87 (4): 459-62.

9.

Takeuchi H and Haltiwanger RS. Significance of glycosylation in Notch signaling.
Biochem Biophys Res Commun 2014 Oct; 453 (2): 235-42.

10. Takeuchi H and Haltiwanger RS. Protein O-glucosyltransferase 1 (Rumi).
Handbook of Glycosyltransferases and Related Genes 2nd ed. 2013.
11.

Takeuchi H.

O-GlcNAc Transferase: Functions, Structure, and Development of Inhibitors.

Hideyuki Takeuchi, Ph.D.

Page-9Trends in Glycoscience and Glycotechnology 2012; 23 (135): 43-45.
12.

Takeuchi H. A new finding in early N-glycan biosynthesis with clinical relevance.
Trends in Glycoscience and Glycotechnology 2010; 22 (128): 311-313.

13.

Takeuchi H and Haltiwanger RS. Role of glycosylation of Notch in development.
Semin Cell Dev Biol 2010; Aug; 21(6): 638-45.

14. Lee TV*, Takeuchi H*, and Jafar-Nejad H. Regulation of Notch signaling via O-glucosylation. Insightsfrom Drosophila studies.
Methods Enzymol 2010; 480: 375-98. (*: Equal contribution)
15. Takeuchi H. Key role of heparan sulfate in life-threatening protein-losing enteropathy.
Trends in Glycoscience and Glycotechnology 2009; 21 (117): 41-43.
16.

Takeuchi H* and Haltiwanger RS. The role of O-glucosylation in Notch signaling.
Trends in Glycoscience and Glycotechnology 2008; 20 (113): 159-170. (*: Corresponding author)

17.

Takeuchi H and Irimura T. Molecular Biology of the Mucins-structure, function, and classificationTan to Sui 2005; 26 (5): 435-440.

18. Takeuchi H, Kato K and Irimura T. Regulation of mucin-type O-glycosylation and its biologicalsignificance.
Seibutsu Butsuri Kagaku 2002; 46(2): 39-44.
19. Irimura T, Denda K, Iida S, Takeuchi H, and Kato K.
potential significance in tumor immunity.
J Biochem (Tokyo) 1999 Dec; 126 (6): 975-85.

Diverse glycosylation of MUC1 and MUC2:

20. Takeuchi H and Irimura T. Structure and function of MUC1 mucin.
Tanpakushitsu Kakusan Koso 1998 Dec; 43 (16 Suppl): 2542-8.
INVITED PRESENTATIONS
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.

The 18th Symposium of Japan Consortium for Glycobiology and Glycotechnology. Tokyo (Online). 2021
December.
The 44th Annual Meeting of the Molecular Biology Society of Japan. Yokohama (Online). 2021 December.
The 94th Annual Meeting of the Japanese Biochemical Society. Yokohama (Online). 2021 November.
The 92nd Annual Meeting of the Japanese Biochemical Society. Yokohama. 2019 September.
The 5th Annual Meeting of the Japan Muscle Society. Tokyo. 2019 August.
The 41st Annual Meeting of the Molecular Biology Society of Japan. Japan. 2018 November.
The Annual Meeting of the Society for Glycobiology. New Orleans. 2018 November.
Benzon Foundation Symposium #64. Copenhagen. 2018 August.
ConBio2017. Japan. 2017 December.
BMB2015. Japan. 2015 December.

Hideyuki Takeuchi, Ph.D.

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11.
12.
13.
14.
15.
16.
17.

Chiba University. Japan. 2015 November.
Gordon Research Conference (Glycobiology). Italy. 2011 May.
GCOE Seminar. The University of Tokyo. Japan. 2011 March.
The Notch meeting. Greece. 2009 September.
Gordon Research Conference (Glycobiology). Ventura. 2009 January.
Annual Meeting of the Society for Glycobiology. Boston. 2007 November.
Annual Conference of the Japanese Electrophoresis Society. Japan. 2001 November.

PRESENTATIONS AT THE INTERNATIONAL CONFERENCES
1.

Takeuchi H, Urata Y, Tsukamoto Y, Saiki W, Senoo Y, Ma C, Wang W, Aoki K, Tiemeyer M, and Okajima
T. Significance of structurally diverse elongation of O-glucose glycans on Notch1 and Notch2. Annual
Conference of the Society for Glycobiology
Glycobiology 2019 Nov. Abstract number 164.

2.

Takeuchi H, Yu H, Hao H, Takeuchi M, Ito A, Li H, and Haltiwanger RS. O-Glycosylation modulates thestability of epidermal growth factor-like repeats and thereby regulates Notch trafficking. The 2018 Gordon
Research Conference on Notch signaling in Development, Regeneration & Disease 2018 Jul.

3.

Takeuchi H, Yu H, Takeuchi M, Ito A, Li H, and Haltiwanger RS. Structural and biochemical analyses
suggest that O-fucose and O-glucose glycans modulate protein folding and flexibility of EGF repeats.
Annual Conference of the Society for Glycobiology
Glycobiology 2016 Nov. Abstract number 115.

4.

Yu H, Takeuchi M, Bakker H, Haltiwanger RS, Li H, and Takeuchi H. Modulating Notch activity withxylosyltransferases: Structure and function analysis of UDP-xylose a1,3-xylosyltransferase-1. The 2014
Gordon Research Conference on Notch signaling in Development, Regeneration & Disease 2014 Jul.

5.

Takeuchi H, and Haltiwanger RS. Can we use Fuc analogs to modulate Notch signaling?
Conference of the Society for Glycobiology
Glycobiology 2013 Nov. Abstract number 128.

6.

Takeuchi H, Jafar-Nejad H, Macnaughtan MA, and Haltiwanger RS. Rumi functions as both a protein Oglucosyltansferase and a protein O-xylosyltransferase. International Symposium on Glyco-minded
Biology of Diseases as a Basis of Pharmaceutical Sciences 2012 Nov. Abstract P28.

7.

Fernandez-Valdivia R, Takeuchi H, Samarghandi A, Lopez M, Leonardi J, Rana NA, Haltiwanger RS, andJafar-Nejad H. Regulation of the Mammalian Notch signaling by the protein O-glucosyltransferase Rumi.
Annual Conference of the Society for Glycobiology
Glycobiology 2010 Nov. Abstract number 25.

8.

Takeuchi H*, Taylor P*, Handford PA, and Haltiwanger RS. Examining how O-fucose and O-glucose
glycans affect Notch-ligand binding. Annual Conference of the Society for Glycobiology
Glycobiology 2010 Nov. Abstract number 111. (*: Equal contribution)

Hideyuki Takeuchi, Ph.D.

AnnualPage-11

9.

Sethi M, Buettner FFR, Krylov V, Takeuchi H, Nifantiev N, Haltiwanger RS, Gerardy-Schahn, and Bakker
H. Identification of the xylosyltransferase adding the second xylose to O-glucosylated Notch EGF repeats.
Annual Conference of the Society for Glycobiology
Glycobiology 2010 Nov. Abstract number 162.

10.

Sethi M, Buettner FFR, Krylov V, Takeuchi H, Nifantiev N, Haltiwanger RS, Gerardy-Schahn, and Bakker
H. Notch xylosyltransferases. Annual Conference of the Society for Glycobiology
Glycobiology 2009 Nov. Abstract number 21.

11.

Takeuchi H, Caswell DS, Jafar-Nejad H, and Haltiwanger RS. Novel specificity of a glycosyltransferase,
rumi, required for Notch signaling. Annual Conference of the Society for Glycobiology
Glycobiology 2009 Nov. Abstract number 161.

12.

Du J, Leonhard CL, Takeuchi H, Shroyer KR, Dlugosz M, Haltiwanger RS, and Holdener BC. OFucosylation of thrombospondin type I repeats maintains pluripotency of epiblast and balances
differentiation during mouse gastrulation. Annual Conference of the Society for Glycobiology
Glycobiology 2009 Nov. Abstract number 173.

13.

Du J, Takeuchi H, Leonhard CL, Dlugosz M, Haltiwanger RS, and Holdener BC. O-Fucosylation ofthrombospondin type I repeats is essential for normal gastulation in mouse embryos. Annual Conferenceof the Society for Glycobiology
Glycobiology 2007 Nov; 17 (11): 1243.

14.

Takeuchi H, Kanoh A, Kato K, Waki M, Usami K and Irimura T. Interleukin-4 induces specific ppGalNAc-T expression and altered mucin O-glycosylation in colonic epithelial cells. Annual Conferenceof the Society for Glycobiology
Glycobiology 2005 Nov; 15 (11): 1247.

15.

Takeuchi H, Usami K, Waki M, Wandall H, Kato K, Clausen H, and Irimura T. Regulation of density ofO-glycans on MUC2 mucin by polypeptide GalNAc-transferases expressed in colon carcinoma cells. Joint
Meeting of the Society for Glycobiology and the Japanese Society of Carbohydrate Research
Glycobiology 2004 Nov; 14 (11): 1163.

16.

Usami K, Takeuchi H, Fujioka K, Takada A, Kawaoka Y, and Irimura T. Interaction of Ebola virus
glycoprotein with human macrophage galactose-type C-type lectin. Joint Meeting of the Society for
Glycobiology and the Japanese Society of Carbohydrate Research
Glycobiology 2004 Nov; 14 (11): 1101.

17.

Takeuchi H, Usami K, Waki M, Wandall H, Kato K, Clausen H, and Irimura T. Recognition of O-glycan
clusters synthesized enzymatically on mucin by lectins.
Annual Conference of the Society for
Glycobiology
Glycobiology 2003 Nov; 13 (11): 890.

18.

Takeuchi H, Kato K, and Irimura T.
Glycoconjugate J 2001 18: 1/2, 32.

Hideyuki Takeuchi, Ph.D.

Recognition of O-glycan clusters on mucins by lectins.

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19.

Takeuchi H, Kato K, Hassan H, Clausen H, and Irimura T. Rules governing O-glycosylation of a cluster
acceptor site of three consecutive threonines: GalNAc-transferase isoforms may be directed by glycocodes.
6th International Workshop on Carcinoma-associated Mucins 2000 Abstract 133.

20.

Iida S, Takeuchi H, Hassan H, Clausen H, and Irimura T. Incorporation of N-acetylgalactosamine into
consecutive threonine residues in MUC2 tandem repeat by recombinant human N-acetyl-D-galactosaminetransferase-T1, T2 and T3.
Glycoconjugate J 1999 16: 4/5, S92.

BIBLIOGRAPHY (Being Acknowledged)
1.

Lee TV, Sethi MK, Leonardi J, Rana NA, Buettner FFR, Haltiwanger RS, Bakker H, and Jafar-Nejad H.
Negative regulation of Notch signaling by xylose.
PLOS Genetics 2013 Jun; 9 (6): e1003547.

2.

Yamamoto S, Charng WL, Rana NA, Kakuda S, Jaiswal M, Bayat V, Xiong B, Zhang K, Sandoval H, DavidG, Wang H, Haltiwanger RS, and Bellen HJ. A mutation in EGF repeat-8 of Notch discriminates between
Serrate/Jagged and Delta family ligands.
Science 2012 Nov; 338 (6111): 1229-32.

3.

Leonardi J, Fernandez-Valdivia R, Li Y-D, Simcox AA, and Jafar-Nejad H. Multiple O-glucosylation sites
on Notch function as a buffer against temperature-dependent loss of signaling.
Development 2011 Aug; 138 (16): 3569-78.

4.

Jafar-Nejad H, Leonardi J, and Fernandez-Valdivia R.
regulation of Notch signaling.
Glycobiology 2010 Aug; 20 (8): 931-49.

5.

Luther KB and Haltiwanger RS. Role of unusual O-glycans in intercellular signaling.
Int J Biochem Cell Biol 2009 May; 41 (5): 1011-24.

6.

Harada Y, Li H, Li H, and Lennarz WJ. Oligosaccharyltransferase directly binds to ribosome at a locationnear the translocon-binding site.
Proc Natl Acad Sci USA 2009 Apr; 106 (17): 6945-9.

7.

Luther KB, Schindelin H, and Haltiwanger RS. Structural and mechanistic insights into lunatic fringe
from a kinetic analysis of enzyme mutants.
J Biol Chem 2009 Jan; 284 (5): 3294-305.

Hideyuki Takeuchi, Ph.D.

Role of glycans and glycosyltransferases in the

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REFERENCES
Tetsuya Okajima, M.D. Ph.D.
Professor
Department of Molecular Biochemistry
Nagoya University Graduate School of Medicine
65 Tsurumai, Showa-ku, Nagoya 466-8550 JAPAN
Phone: +81-52-744-2068
Email: tokajima@med.nagoya-u.ac.jp
Tatsuro Irimura, Ph.D.
Professor emeritus (The University of Tokyo)
Professor (Biochemistry and Breast Surgery)
Juntendo University School of Medicine
2-1-1 Hongo, Bunkyo-ku, Tokyo 112-8421 JAPAN
Phone: +81-3-3813-3111
Email: tats@mbr.nifty.com
Robert S. Haltiwanger, Ph.D.
GRA Eminent Scholar in Biomedical Glycosciences
Professor of Biochemistry and Molecular Biology,
Complex Carbohydrate Research Center, The University of Georgia.
315 Riverbend Road,
Athens, Georgia 30602 USA
Phone: +1-706-542-4151
FAX: +1-706-542-4412
Email: rhalti@uga.edu
Hamed Jafar-Nejad, M.D.
Professor
Department of Molecular and Human Genetics, Baylor College of Medicine.
One Baylor Plaza, Room 919E, BCM225
Houston, Texas 77030 USA
Phone: +1-713-798-6159
FAX: +1-713-798-8142
Email: hamedj@bcm.edu
Pamela Stanley, Ph.D.
Horace W. Goldsmith Chair
Professor
Department of Cell Biology, Albert Einstein College of Medicine.
1300 Morris Park Ave.,New York, New York 10461 USA
Phone: +1-718-430-3346
Fax: +1-718-430-8574
Email: pamela.stanley@einstein.yu.edu

Hideyuki Takeuchi, Ph.D.

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