能機超高 ンパク質

開発のスー
データベ

構造タ

gaou@sfc.keio.ac.jp
112

2

1

荒川和晴，河野暢明，中村浩之，大利麟太郎，冨田勝，沼田圭司

1. 慶應義塾大学先端生命科学研究所

2. Spiber Inc.

3

3. 理化学研究所 環境資源科学研究センター

Abstract
クモ糸などの生体由来構造タンパク素材は、その強靭さや再生可能性などから、次世代の高機能素材として注目されている。我々は革新的研究開発推進プログラム（ImPACT・鈴木PM）の一部として、クモ糸を中心とした超高機能構造タンパク質の高機能発現メカニズムを明らかにするために、世界中からこれらの遺伝子を集め、また、タンパク自体の物性を詳細に解析し、データベース化を進めている。本プロジェクト終了時には、1,000種類を超えるクモ糸を始めとした構造タンパク配列と、250以上の構造タンパクの網羅的物性データをオープンに公開する予定である。本データベース
は本質的に多様な生物群のサンプリングに関連した生態情報、核酸及びアミノ酸配列情報、そしてタンパクの物性情報など多岐に跨がる情報との連携が必須であり、そのためにResource Description Framework（ RDF/XML）に基づくデータ出力も実装予定である。

クモ糸 遺伝子の 進化 と発現される超高機能 物性を紐付け

次世代シーケンサー による配列解析

Blackledge et al. (2012) Sci. Rep.

Garb et al. (2010) BMC Evol. Biol.

Spring-8 などによる物性測定

samples

応力

1,000world-wide

物性発現

ひずみ

デザインを可能に

Comprehensive measurement of Spider Silkome

望みの物性を持つ超高機能タンパク素材のSpider Silk: a dream materialHigh Strength
Low Extensibility

・鋼の4倍の強度
・ナイロンを上回る伸縮性
・最も”タフ”な素材
Low Strength
High Extensibility
・300℃までの熱安定性・再生可能なタンパク素材
Extensibility
toughness = area under the curve(steel, carbon fiber, aramid fiber)StrengthHigh Toughness
Mid. StrengthMid. Extensibility
Unprecedented material(synthetic rubber, polyester, nylon)

微生物発酵で人工クモ糸が量産可能に。

Latrodectus hesperus clone 16D22 major ampullate spidroin 1 (MaSp1) gene, complete cds
GenBank: EF595246.1

クモ 59科237属※ 参考：日本産クモ類は約1500種64科
Top Page

Entity Page
BannerBannerNephilidae > Nephila! WSC

1000 Samples
200 Mechanical Properties
100 Morphology
50 Structural Information! NCBI

Tensile Strength (GPa)Nephia clavata L. Koch, 1878

Advanced Search

40 Thermal Properties
Strain at break (%)

IxodesCentruroides
MastigoproctusDamon
Liphistiidae
Atypoidina*

Mecicobothriidae
Nemesiidae

***

*

**

Theraphosoidina
Paratropididae

IDV#4682

Nemesiidae in part*

Sampling date: 2016/11/1

Ctenizidae

Sex: Female

Idiopidae
Euctenizidae*

Browse Properties"

Browse Sequences"

Browse Organisms!

Hypochilidae
Filistatidae
Caponiidae
Dyseridae
Segestriidae
Sicariidae
Scytodidae
Diguetidae

Crystalinity
15.8

Pholcidae
Leptonetidae
Eresidae
Theridiidae
Mysmenidae
Mimetidae
Tetragnathidae
Nesticidae
Pimoidae
Linyphiidae
Nephilidae
Araneidae
Oecobiidae
Uloboridae
Deinopidae

About UsLorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown
printer took a galley of type and scrambled it to make a type specimen book. It has survived not only ﬁve centuries, but also the leap into electronic typesetting, remaining essentiallyunchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus

Diameter
2.68±0.03

Strain at break

16.9±2.72%

1 % weightross

N.D.

℃

Tensile Strength

1.12±0.14

GPa

5% weightross

N.D.

℃

Young Modulous

9.05±2.08

GPa

10% weightross

N.D.

℃Toughness

0.092±0.024 GJ / m ^ 3

Transition temperature

N.D.

℃

Birefringence52.6 ± 10.9

Supercontraction

N.D.

これらは全て単系統。進化を追うことで高強度、高タフネス、高伸縮など、さまざまな物性を持つ配列が得られる。

Nature is full of high-performance protein materials - Not just Spider Silks✔️ ７種類のクモ糸タンパク✔️ ミノムシの糸フィブロイン
✔ ️天然ゴムを上回る伸縮性のレジリン✔ ️天然セラミック・ピラルク鱗のコラーゲンMSWSTLALAILAVLSTQSIYASAQARSPWSDTATADAFIQNFLAAVSGSGAFSSDQLDDMSTIGDTIMSAMDKMARSNKSSQHKLQALNMAFASSMAEIAAVEQGGMSMGVKTNAIADALNSAFYMTTGAANPQFVNEMRSLISMISAASANEVSYGGGASAAAAAAAGGYGQGPSGYGQGPSASSAAAAAPSGYGPSQQGPSGSAAAAAAAAGPAQQGPSAYGPSGPGAAAATAAAGPGGYGPQQGPGPQGPSGYGPSGPGSAAAAAAAAGAGPGGYGPQQQGPGQQGPSGYGPSGPSGPGSAAAVAAATGAGPGGYGPGQQGPGGYGPGQQGPSGPGSAAAAAAAAAGAGPRGYGPGQQGPAGQGPTGYGPSGPGSAAAAAAGAGPGQQGPGGYRPSGQGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPSGPGGAAAAAAAGPGGYGPGQQGPSGPGSAAAAAGAGPAGYGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPRGYGPGQQGPNGPGSAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPSGYGPSGPGSAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGTAAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPSGPGGAAAAAAGPGGYGPGQQGPGGYGPSGSGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPLQQGPGQQGPGGYGPSGSGSAAAAAGGAGPGGYGPGQQGPGQQGPSGYGPSGPGSAAQQAAAAAAGAGPGGYGPGQQGPGGYGPSGPGSAAAAAAAAGAGPRGYGPGQQGPGQQGPPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQRGPGGYGPSGPGSAAAQAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAGPGGYGPGQQGPSGPGSAAAAAGAGPGGYGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGP2017/03/01: Lorem Ipsum

クモは複数種の糸を作り、それぞれ幅広い物性を示すが、MaSp2

Contrary to popular belief, Lorem Ipsum is not simply random text. It has roots in a piece of classical Latin literature from 45 BC, making it over 2000 years old. Richard McClintock, aLatin professor at Hampden-Sydney College in Virginia, looked up one of the more obscure Latin words

Vollrath et al. (2006) Soft Matter%

PageMaker including versions of Lorem Ipsum.

News, Update3129 aa.

MTWSTRLALSFLFVLCTQSLYALAQANTPWSSKANADAFINSFISAASNTGSFSQDQMEDMSLIGNTLMAAMDNMGGRITPSKLQALDMAFASSVAEIAASEGGDLGVTTNAIADALTSAFYQTTGVVNSRFISEIRSLIGMFAQASANDVYASAGSSGGGGYGASSASAASASAAAPSGVAYQAPAQAQISFTLRGQQPVSYGQGGAGPGGAGAAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGSGAAAAAAAAAGGTGQGGAGQGGAGAAAAAAAAAGGAGQGGQGGYGQGGYGQGGTGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGYGQGGSGAAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAGAAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAAAAAGAGQGGYGGQGAGQGGSGAAAAAAAAGGAGQGGQGGYGQGGYGQGGSGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAAGGAGQGGYGRG
GAGQGGAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGDYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAASAAAAGGAGQGGYGRGGAGQGGAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAATAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAAAAAGAGQGGYGGQGAGQGGAGAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAGAGQGGYGGQGAGQGGAGAAAAASRGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAAAAAGSGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGTAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGASQGGQGGYGQGDYGQGGAGQGGAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGRGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGTGQGGAAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGYGQGGAGQGGAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAAAAAGSGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGYGQGGAGQGGAAAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGNGQGGAGQGGAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGASQGGQGGYGQGDYGQGGAGQGGAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGRGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGRGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAVGGAGQGGYGRGGAGQGGAAAAAAAAAAGSGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGGGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAAGGAGQGGYGRGGAGQGGAAAATGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAGGAGQGGYGRGGAGQGGAAAAAAAAAGAGQGGYGGQGAGQGGAGAAAAAAAAGGAGQGGQGGYGRGGYGQGGAGQGGAGAAAAGGAGQGGQGGYGQGGYGQGGAGQGGAAAAAAAAAAGGAGQGGYGGYGQQGGAGAAAAAASGPGQIYYGPQSVAAPAAAA
ASALAAPATSARISSHASALLSNGPTNPASISNVISNAVSQISSSNPGASACDVLVQALLELVTALLTIIGSSNIGSVNYDSSGQYAQVVTQSVQNAFASchema Diagaram

GGYGPGQQGPGQQGPGQQGPGGYGPSGTGSAAAAAAAAGAGPGGYGPGQQGPGQQSPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGQQGPGQQGPG2017/03/31: Lorem Ipsum

VYGPSGPGSAAAAAASAGAGPGGYGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQDQGAG2017/04/01: Lorem IpsumPGGQQGPGQQGTTGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGQQGPGQQGPVQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAAAGPGGYGPGQQGPGGYGPSGPGSAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGTQGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPVGYGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGP

Web site in development. The database will befully open upon publication, with RDF markupfor non-image data.

GQQGPGQQGPGQQGPSGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAATGAGPGGYGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGGQQGPGGYGPSGPGSSAAAAAAAAAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGQQGTGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGQQGPGGYGPSGPGSAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPRQQGPGQQGPRQQGPGGYGPSGPGSAAAAAAATGAGPGGYGPGQQGPGQQGPGGYGPSGPSGPGGAAAAAAGAGPGGYGPGQQGPSGPESALAAAGAGPGGYGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPSGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGQQGPGQQGPGGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSSAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAGAGPGGYGPGQQGQGQQGPGGYGPSGPGSSAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSSAAAAAAAGAGPGGYGPGQQGPGQQGPGGYGPSGPGSAAAAAAAASAGPGGYGPGLQGPGQQGLAGYGPSGPGSAAAAAAAAGAGPGGYGPGQQGPSGPGSAAAAAAGSGPGGYGPGQQGPGGYGPSGPSGPGGAAAAAAAAAAGPGGYGPGQQGPAGYGPSGPSVPGGAAAAAAGPGGYGPGQQGPSGPGNAAAAAAGAGPGGYGPGQQGPGGYGPSGPSGPGGAAAAAAAAAGPGGYGPGQQGPAGYGPSGLSGPGGAAAAAAAAAGPGGYGPGQQGPSGPGGAAAAAAAAGPGGYGPSQRGPSGPGSAAAAAAGAGPGGYGPGQQGPSGPGSAAAAATGAGAGGYGPGQQGPGGYGPSGPSGPGGASAAAAAAAGPGGYGPGQQGPGQQGPGQQGPAGYGPSGPSGPGGAAAAAAAAAGPGGYGPGQQGPGQQGPGQQGPAGYGPSGLSGPGGAAAAAAAGPGGYGPGQQGPSGPGGAAAAAAAAGPGGYGPSQRGPSGPGSAAAAAAGAGPGGYGPGQQGPSGPGSAAAAAAAGPGGYGPSQQGPARYGPSGPGSAAAAAAGAGTAGYGPGPQASAAASRLASPDSGARVASAVSNLVSSGPTSSAALSSVISNAVSQIGASNPGLSGCDVLIQALLEIVSACVTILSSSSIGQVNYGAASQFAQVVSQSILSAF*MaSp1a> 5,000 構造タンパク遺伝子>

250 糸サンプル

13 種類の物性
3 種類の画像

世界最大の構造タンパク質データベース
would like to thank Yuki Takai, Nozomi Abe, Naoko Ishii, and Yuki Onosawa
Acknowledgements: Wefor technical assistance, and Kayoko Kurimotoi for administrative assistance.

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