Photon Factory Activity Report 2018 #36 (2019)

BL-11A/2018G129

Sample Observation by Talbot Optics installed Soft X-ray Projection Microscopy
1

Tatsuo SHIINA1*, Prane Mariel Basco ONG1, Yasuhito KINJO2, and Atsushi ITO2
Graduate School of Advanced Integration Science, Chiba Univ., Chiba-shi, Chiba, 263-8522, Japan
2
School of Engineering, Tokai Univ., Hiratsuka-shi, Kanagawa, 259-1292, Japan

1 Introduction
To extend the application of soft X-ray projection
microscopy, we tried to incorporate Talbot optics intoprojection microscopy to obtain phase information that isnot accessible by normal projection microscopy. Last year
we installed a grating and a nano-positioning stage into thecurrent soft X-ray projection microscopy system.[1] Thistime, fundamental samples were analyzed with 6 fringe
shifted images to obtain the phase image.
2 Experiment
The setup of Talbot optics installed at soft X-ray projection
microscope is shown in Fig.1. The pinhole of 1µm or 2µm
diameter was utilized as an imaginary light source. The
grating with 2µm interval was put after the pinhole withthe distance determined by Talbot optics. They were
produced by Silson Ltd and had the uniform grating fringe
patterns. The grating was shifted perpendicular to theoptical axis with 0.4µm interval. The sample can be moved
within the distance of 4mm to the grating. It enables themagnification of images. The projection of this microscopeis effective, and the sample is magnified 40 – 76 times
depending on the sample position. The experiment wasconducted using soft X-rays of 1keV at BL-11A. Talbot
effect is self-imaging effect to make the intensity
distribution due to the grating period at a certain distance.
Furthermore, it can extract the phase information byshifting the grating fringe with a certain phase interval.
Figure 2 shows the observation example of partiallyoverlapped Mylar films and slice sample of a human hair.
Partially overlapped Mylar film of 1.5µm thickness wasobserved at 40 times magnification (Fig.2(a)). The
different transmission intensities were indicated inobservation image(i) and their phase image is shown in (ii).
Here, there was no film in ①, single layer in ②, ③ anddouble layer in ④. Each area in both images was uniform.
The difference of intensities in each area is clear inobservation image(i). Phase shift in each area looks smallin phase image(ii), while it depended on the layer thickness.
Human hair slice sample was observed at 76 times
magnification (Fig.2(b)). In observation image(i), medulla(center part) had clearly different transmission intensity
than cortex (surrounding part). In phase image(ii), phase
distribution of medulla part is different from thetransmission intensity distribution. It come from theirstructure. The cortex part had the unique distribution
reflected by slicing thickness. They seem to have thedifferent phase orientation. We are continuing theimprovement of the analysis procedure.

Fig.1 : Talbo optics installed soft X-ray projection
microscopy.

(a) Mylar film(b) human hairFig.2 : Observation of Mylar film and human hair slicedsample.
References[1] T. Shiina et al., PF Activity Report 2018 #36 (2019).
http://pfwww.kek.jp/acr/2018pdf/u_reports/pf18b0261.pdf* shiina@faculty.chiba-u.jp