Sensitive. Accurate. Flexible.
Agilent Cary Eclipse fluorescence spectrophotometer

Agilent Cary Eclipse
Fluorescence Spectrophotometer
Agilent is your premier resource and partner formolecular spectroscopy. The world-renowned
Cary product line, encompasses FTIR,UV-Vis-NIR, and fluorescence instruments,
offering you a comprehensive range ofmolecular spectroscopy solutions.

Answers you can trust
The Cary Eclipse fluorescence spectrophotometer issensitive, accurate, and flexible, and is designed to meetyour immediate and future challenges. With accurate
temperature control, no sample photo bleaching, and arange of measurement options, you can be sure that the
Cary Eclipse will give answers you can trust.
–– Lowest cost of ownership—with a lifetime of 3 billion
flashes, the lamp typically lasts 10 years. This longevity
minimizes lamp replacement, saving you money overthe lifetime of the instrument.
–– No need for cuvettes—the optional fiber-optic probe
delivers accurate results in a fraction of the time,
improving your workflow and reducing your costs.
–– Fast data collection—with a scan rate of up to 24,000
nm/min, you can scan the wavelength range in under3 seconds and collect 80 points per second for kinetics
measurements.
–– Sensitivity—detect picoMolar amounts of fluoresceinin both standard and micro cuvettes.
–– Measure precious or biological samples withease—the xenon flash lamp enables highly sensitive
measurements on small volume samples to be madewithout sample degradation.
–– Flexibility—choose from fluorescence,
phosphorescence, chemiluminescence orbioluminescence collection modes, to provide a robust
and versatile workhorse for all your analytical needs.

2Superior optical design

Extended sensitivity

Small footprint

Fast scanning

Excellent sensitivity is a
result of using a unique,intense xenon flash lamp,
coupled with optimized
grating blaze angles andcoatings which ensuresensitivity across thewhole wavelength range.
Photosensitive samples are
not exposed to continuous
light as the xenon flash
lamp flashes only toacquire a data point.

Red-sensitive
Photomultiplier tube
detectors extend
sensitivity up to 900 nm
without sacrificing
UV performance.

The Cary Eclipse occupies
only 600 mm (24 inches)of bench space.

The Cary Eclipse scans at
24,000 nm/min without
peak shifts due to themonochromator drive
mechanism design. The grating
is moved only when the lamp
is off, resulting in a go-stopflash method of taking ameasurement. The wavelength
does not change while ameasurement is being taken.

In built filters

Large sample
compartment
The large sample
compartment makes
it easy to install andremove accessories,
and fit your own
research apparatus.

Built-in filters on bothmonochromators meansthat you don’t have to
buy your own to minimize
scattering or exclude
second-order stray light.

Room light immunity
If your sample doesn’t fit in
the sample compartment,or it is easier to work with anopen sample compartment,
then simply leave the lid
off. The unique xenon flash
lamp and sophisticatedsignal processing give theCary Eclipse room light
immunity for fluorescencemeasurements.

Measure microvolume
samples

Superfast datacollection

Reduced volume cuvettes
as low as 5 μL areavailable. The horizontal
beam profile ensures
excellent sensitivity evenwith such low volumes.

Scan the whole wavelength
range in less than 3 seconds.

3

Quality and Performance by Design
Our proven record of optical design
excellence and innovation ensures that you
get the right answer every time.

The power of xenon
With unique xenon flash lamp technology, the Agilent
Cary Eclipse is:–– Room-light immune—the unique optical design
enables measurements to be made with the sample
compartment open, allowing large or odd-shaped
samples to be measured.
–– Flexible—the highly focused beam provides superior
coupling to fiber optics, making the Agilent Cary
Eclipse the best choice for fluorescence fiber optic
measurements.
–– Efficient—the lamp only flashes when a reading istaken. This means zero warmup time, low electricalenergy use, and low maintenance requirements.
Photodegradation is also eliminated, as photosensitive
samples are not excessively exposed to light.

Signal-to-noise
Signal-to-noise (S:N) mode enables you to control the levelof precision you want across the whole scan. It is usefulfor samples that vary significantly in emission intensity
across the wavelength range.
S:N mode reduces scanning times by over 50% as thesystem scans quickly in areas of high emission intensity
and increases signal averaging when the emission is less.

4

Superior optics
The Schwarzschild collection optics capture a large portion of
the light from the powerful xenon flash lamp and direct it throughthe sample, resulting in excellent sensitivity and low signal noise.

Distinctly Better Software
User friendly, application focused software
provides complete instrument control.

Software designed for real samples
The modular design of the Cary WinFLR software meansthat it can be tailored to suit your analytical requirements.
The software modules include basic wavelength scans orconcentration measurements, through to measurements
to suit life science applications requiring advanced
polarization or thermal control.

Dedicated software applications
Streamline your measurements and save time with the easy-to-use
Cary WinFLR software. Investigate intracellular ion transfer processes
using the Fast Filter module or study drug binding assays using
kinetics and polarization.

Obtain 3-D graphs and contour plots in seconds
Automatically collect a series of excitation, emission, or synchronous
scans in all x modes. The 3-D data can be sliced to produce single
excitation or emission scans, or contour plots can be created to show
the number of emitting species.

Enhanced graphics features
The graphics control module has automatic peak labeling, zoom,
free and tracking cursor, multiple ordinate and abscissa formats.
It also has smart copy/paste and overlay modes, making spectralinterpretation and presentation for publications a breeze.
Advanced data processing
Use the spectrum calculator to apply mathematical operations,
including addition, subtraction, division, multiplication, log, andsquare root functions, to spectra. The calculator also features mean,
normalization, smoothing, up to fourth order derivatives,and integration algorithms.
Meet your application challenges
Use the powerful built-in Applications Development Language (ADL)
to tailor the Cary WinFLR software to meet your most specificapplications.

5You can do it all with a Cary
The Cary Eclipse instruments are complemented
by a range of accessories and software
designed specifically for your application needs.

Performance enhancing accessories

High throughput microplate reader

The vast range of accessories for the Cary Eclipse
ensures you can handle the widest variety of sample
sizes and types.

The microplate reader accessory turns the Cary Eclipse
into a high throughput microplate reader in less than
30 seconds. It provides full wavelength scanning withexcellent sensitivity using reflective optics instead offiber optics.

Accessories for liquid samples–– Microplate reader for method development or highthroughput measurements.
–– Fiber-optic probes and couplers for fast accurate
measurements without cuvettes.
–– Peltier and water thermostatted single and multicell
holders for precise temperature control.
–– Temperature probes for accurately measuring thetemperature inside the cuvette.
–– Rapid mix accessory for investigating ultrafast kinetics
measurements that are over within seconds.
–– Manual and automated polarizers for excitation down
to 275 nm.
Accessories for solids, powders, and pastes–– Solid sample holder to acquire fluorescence spectraof many sample types, including filters, powders, gels,
optical components, and fabrics.
–– Fiber optic reflectance probe and coupler.

6–– Measure 96 wells in less than 50 seconds and
384 in less than 90 seconds.
–– Perform full wavelength scans on each well withinminutes. Measure in steady-state fluorescence,
phosphorescence, bio-/chemi-luminescence, or
time-resolved delayed fluorescence modes.
–– Measure minute sample quantities deposited on
the sides or base of the wells.
–– Customize the measurement positions for
nonstandard microplates or substrates. Control theimage spot size to as small as 2 mm in diameter.
–– Automatically align the excitation beam on yourmicroplates and store information about eachplate type.
–– Measure samples such as gels, films, and solids
at various locations on their surface by using the
microplate reader as an x-y transport.

140
120

Intensity (a.u.)

100
80
60
40
20
0
570

Monitor temperature control
The temperature probe enables the temperature inside the cuvette to bemeasured, providing the most accurate data for temperature-dependent
experiments. The Cary WinFLR software monitors the temperature
directly from probe, ensuring data is collected at the correct temperature.

580

590

600
610
Wavelength (nm)

620

630

640

Scan cells adhered to wells
The microplate reader can easily scan cells adhered to the side ofa well, with excellent signal-to-noise. This figure shows an emission
scan of Rhodamine B which has been coated to the side and bottomof a 384 well white microplate.

7Chemicals and Materials Applications
When you need to consistently and cost-effectively deliver quality finished products, innovative,
reliable analytical solutions are essential to your success. The Cary Eclipse enables minimal
sample preparation and versatile sampling solutions.

Flexible sampling

150

Combine the Cary Eclipse with fiber-optic probes
to create a sensitive, remote-reading fluorescence
spectrophotometer:–– Room light immunity means that there is no restriction
on sample size or shape

Intensity (a.u.)–– Use the fiber optic system to measure emission fromthe surface of a solid or from a liquid

100

50

Superior scanning–– Rapid scan rate of 24,000 nm/min without peak shifts
is due to the design of the monochromator drive
mechanism. The grating only moves when the lamp isoff, resulting in a go-stop-flash measurement method—
the wavelength does not change while a measurement
is being taken.
–– Use Computer Averaging of Transients (CAT) scanmode to average several individual scans until
you are satisfied with the signal-to-noise quality.

8

0
500

550
Wavelength (nm)Fiber optics measurements
The Cary Eclipse fiber optic system can be used to measure theemission from the surface of a solid or that emitted by a liquid,
without compromise in data quality.

Solid sample measurements
Use the solid sample holder to easily measure the fluorescence
properties of samples—from optical filters, paints, and fabric tospecialty chemicals such as optical brighteners.

Fluorescence measurement of detergents

Fluorescence measurement of stalactites

Use the Cary Eclipse fluorescence spectrophotometer withsolid sample holder to measure the fluorescence of opticalbrighteners in laundry detergents.

Use the Cary Eclipse fluorescence spectrophotometer withfibre optic coupler and probe to measure the fluorescenceof demanding solid samples such as stalactites.

–– Solid sample holder is easy to install and align,
minimizing sample preparation.

–– Measure oddly shaped samples such as stalactites
and live corals using an optical light guide.

–– Use in combination with the powder holder and edge
mounting kit for even greater solid sampling flexibility.

–– Fiber-optic probe and coupler are easy to install andalign and require no sample preparation.

–– Acquire spectra with the sample compartment open.

–– Easily acquire fluorescence spectra—simply place thesolids tip on the surface of the sample without any
need for light shielding.

1000

1000

380
861

370

400

730

350
Wavelength (nm)Intensity (a.u.)

Intensity (a.u.)

600

796

360

800

800

600

400

664

340

599

330

533

320

467

310

402
336

300

270

290
200

200

205

280

139

270
0

200

300

400
Wavelength (nm)

500

600

Examine the fluorescent properties of a common laundry detergent
The excitation and emission spectra of the powder show opticalbrighteners absorbing in the region between 320 and 390 nm andemitting over the 400–500 nm range. Fabrics washed in this detergent
would exhibit a blue hue.

73
8

260
400

450 500 550
Wavelength (nm)600

400 425 450 475 500 525 550 575 600

Wavelength (nm)Investigating the fluorescent properties of a crosssectioned stalactite
The probe tip was positioned on the stalactite surface to collectthe excitation emission matrices (EEMs). The system can also be
used for gemstone fingerprinting and impurity detection, and for soil,
mineral and ore analysis.

9Life Science Applications
In a field that demands accuracy and productivity, your challenges have never been greater.
Today, analysis must be done more reliably, more efficiently, and with even higher quality results
than ever before. Agilent provides unrivalled optical performance and superior temperature
control to measure the most challenging of samples with the highest accuracy.

–– Micro cuvettes enable highly accurate measurements
of precious biological samples.
–– Sample compartment temperature is stable, as thelamp does not produce heat, ensuring accurate andreproducible data.

Rapid and precise kinetics measurements–– Collect steady-state fluorescence data at 80 points
per second, and pause data collection at any time to
add reagents without affecting performance.
–– Extend collection times during a run.
–– Perform time-resolved phospherence and delayed
fluorescence lifetime measurements.
–– Use the rapid mix accessory to analyze reactions thatare over in less than 1–2 seconds.

Intracellular ion concentrations–– Use the fast filter accessory or the fast slew rate of
the monochromators to collect data for intracellular
ion concentration analysis or pH measurements inreal time. This could be 50 ms to 1 second forratiometric measurements or every 12.5 ms for single
wavelength dyes.

10

B 100

60
50

Intensity (a.u.)–– Photosensitive samples are not exposed to continuous
light as the lamp flashes only to acquire a data point,preventing photodegradation.

A
Intensity (a.u.)

Protect precious samples

40
30
20
10
0
400

450
500
Wavelength (nm)

550

0
400

450
500
Wavelength (nm)550

No photo bleaching
Emission wavelength vs intensity for BFP following 370 nm excitation.
A negligible drop in peak BFP emission (450 nm) was recorded after
10 successive scans at a scan rate of 120 nm/min (total exposure time
12 min 30 s) using the Cary Eclipse (a). Photobleaching ofapproximately 20% was observed by using a commercially available
instrument fitted with a traditional xenon arc lamp (b).

Automated polarizer
Simplify measurements by using the automated polarizer
and Cary WinFLR software to automatically measure changes
in polarization over time.

Rotational motion of molecules

Excellent temperature control–– Optional UV transmitting film polarizers enable
excitation down to 275 nm, ensuring that eventryptophan can be excited without photobleaching.

–– The Cary Eclipse temperature controlled peliterthermostatted cell holder offers:–– Polarizers allow measurements to be obtained at thesignificant angle of 55˚ as well as the complimentary
angle of 35˚.

–– Rapid and precise temperature control essential forcontrolling the intensity of fluorescence emission–– Simultaneous measurement of up to four samples–– With a low extinction ratio in the cross-position,
rotational motion of proteins and solvent interactions
can be measured with high precision and accuracy.
–– Sturdy design ensures the polarizers are easy to clean
and maintain.

–– Minimal cell-to-cell variation (maximum difference,
0.2 °C at 37 °C)–– Accurately measure the temperature of the actual
sample within the cuvette using temperature probes–– In-built electromagnetic stirring providing complete
control of the stirring speed, with no fluctuations(up to 4 cells)

80–– Temperature ramp rates as slow as 0.06 °C/min canbe selected for thermal denaturation and renaturation
studies of DNA via fluorescence resonance energy
transfer (FRET)70

Intensity (a.u.)–– Excellent stability control over time (typical variation±0.05 °C)

60
50
40
30
20
450

500
Wavelength (nm)550

Monitor cell function of photosensitive samples at the protein level
Emission spectra of the blue fluorescent protein (BFP)- green
fluorescent protein (GFP) fusion protein following 360 nm excitation
are shown. GFP emission (~510 nm) is seen upon specific excitationof BFP alone (360 nm), indicative of FRET.

11

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This information is subject to change without notice.
© Agilent Technologies, Inc. 2019
Published in the USA, July 8, 20195990-7788EN