助教

水口 千穂(みなくち ちほ)
Room 203
Phone: [03-5841-3064] Fax: [03-5841-8030]
E-Mail: [ucsmina@mail.ecc.u-tokyo.ac.jp]

略歴  20092011
2014

学士修士
博士

東京大学
東京大学
東京大学

2014

東京大学

農学部 応用生命科学課程生命工学専修
農学生命科学研究科 応用生命工学専攻
農学生命科学研究科 応用生命工学専攻

生物生産工学研究センター 助教

研究内容  有機化合物により汚染された土壌・水の浄化法の一つに、微生物を用いたバイオレメディエーションが挙げられます。私達は環境細菌の分解活性を引き出すため、分解菌のスクリーニングや分解遺伝子群の同定、各種遺伝子の転写制御機構について研究を行っています。また分解遺伝子の多くがプラスミドにコードされていることから、プラスミド保持が宿主の生育や代謝に与える影響や、プラスミド-宿主染色体間相互作用の鍵因子である核様体タンパク質が転写制御ネットワークに与える影響についても分子レベルで解析を進めています。

主要論文  
Suzuki-Minakuchi C, Hirotani R, Shintani M, Takeda T, Takahashi Y, Matsui K, Vasileva D, Yun CS, Okada K, Yamane H,Nojiri H (2015) "Effects of three different nucleoid-associated proteins encoded on IncP-7 plasmid pCAR1 on hostPseudomonas putida KT2440." Appl. Environ. Microbiol. 81:2869-80
2.
Shintani M, Suzuki-Minakuchi C, Nojiri H (2015) "Nucleoid-associated proteins encoded on plasmids: Occurrence andmode of function." Plasmid 80:32-44
3.
Yun CS, Takahashi Y, Shintani M, Takeda T, Suzuki-Minakuchi C, Okada K, Yamane H, Nojiri H. "MvaT family proteinsencoded on IncP-7 plasmid pCAR1 and the host chromosome regulate host transcriptome cooperatively butdifferently." Appl. Environ. Microbiol. in press
4.
Kotake T, Matsuzawa J, Suzuki-Minakuchi C, Okada K, Nojiri H, Iwata K. "Purification and partial characterization ofthe extradiol dioxygenase, 2'-carboxy-2,3-dihydroxybiphenyl 1,2-dioxygenase, in the fluorene degradation pathway
from Rhodococcus sp. strain DFA3." Biosci. Biotechnol. Biochem. in press
5.
Matsuzawa J, Aikawa H, Umeda T, Ashikawa Y, Suzuki-Minakuchi C, Kawano Y, Fujimoto Z, Okada K, Yamane H, NojiriH (2014) "Crystallization and preliminary X-ray diffraction analyses of the redox-controlled complex of terminal
oxygenase and ferredoxin components in the Rieske nonhaem iron oxygenase, carbazole 1,9a-dioxygenase." Acta
Crystallogr. F Struct. Biol. Commun. 70:1406-9.
6.
Yee L, Hosoyama A, Ohji S, Tsuchikane K, Shimodaira J, Yamazoe A, Fujita N, Suzuki-Minakuchi C, Nojiri H (2014)"Complete genome sequence of a dimethyl sulfide-utilizing bacterium, Acinetobacter guillouiae strain 20B (=NBRC110550)." Genome Announc. 2:e01048-14.
7. Suzuki C, Kawazuma K, Horita S, Terada T, Tanokura M, Okada K, Yamane H, Nojiri H (2014) "Oligomerization
mechanisms of an H-NS family protein, Pmr, encoded on the plasmid pCAR1 provide a molecular basis for functionsof H-NS family members." PLOS ONE 9:e105656
8. Matsuzawa J, Umeda T, Aikawa H, Suzuki C, Fujimoto Z, Okada K, Yamane H, Nojiri H (2013) "Crystallization andpreliminary X-ray diffraction studies of the reduced form of the terminal oxygenase component of the Rieske
nonhaem iron oxygenase system carbazole 1,9a-dioxygenase." Acta Crystallogr. F Struct. Biol. Commun. 69:1284-7
9.
Suzuki C, Yun CS, Umeda T, Terabayashi T, Watanabe K, Yamane H, Nojiri H (2011) "Oligomerization and DNA-binding
capacity of Pmr, a histone-like protein H1 (H-NS) family protein encoded on IncP-7 carbazole-degradative plasmid
pCAR1." Biosci. Biotechnol. Biochem. 75:711-7
10. Yun CS, Suzuki C, Naito K, Takeda T, Takahashi Y, Sai F, Terabayashi T, Miyakoshi M, Shintani M, Nishida H, Yamane H,Nojiri H (2010) "Pmr, a histone-like protein H1 (H-NS) family protein encoded by the IncP-7 plasmid pCAR1, is a key
global regulator that alters host function." J. Bacteriol. 192:4720-31
1.