FLEXcyte 96
True contractility

FLEXcyte 96

Contractility under physiologicalconditions
FLEXcyte 96 transforms traditionally limited cardiac

Unique flexible substrates of the FLEXcyte

contractility approaches of the Langendorff Heart

recording plates, mimic in vivo-like environments.

into a cutting-edge modern high-throughput

Human induced pluripotent stem cell-derivedtechnique.

cardiomyocytes (hiPSC-CMs) grown in suchphysiological conditions display positive inotropic
responses without additional stimulation.

Langendorff Heart
Adult cardiac phenotype

Target species

Throughput

Measured parameters

Genetic disease model

FLEXcyte 96

FLEXcyte 96

Key features at a glance
1

Real contractility measurements

5

Monitoring of acute and chronic effects2

Non-invasive and label-free

6

Optical pacing3

96 recordings in parallel

7

Real-time access to beating parameters

4Pro-maturation environment

8

Ideal for cardiotox and efficacy studies

FLEXcyte 96

Efficient data handling and export
Data recording and analysis software

Data Control 96, accelerates data analysis bypackage available for FLEXcyte 96 supports

quick and easy loading of recorded files and user

online analysis of beating parameters anddefined analysis templates.

quantifies contractile force of cardiomyocytes.
The unique Mean Beat Function automatically• Contractile force (mN/mm2) detection

visualizes the average contractility pattern within• Instant recalculation of data upon parameterchangesthe recorded well, enveloped by the standard
deviation, indicating the synchronicity of thebeating cellular network.

• Broad range of QC options• Templated data analysis allows for results within
seconds• Highly customizable exporting to databases

Analysis with DataControl 96
Scroll through the data, select adata analysis template or fitting.
Results, compound information and
QC parameters are exported in auser-defined export format, ready forfurther database integrations.

FLEXcyte 96

Flexibility is key to maturation
What is a pro-maturation environment?

FLEXcyte 96 Sensor Plate

Cardiomyocytes constantly sense their

Sophisticated FLEXcyte plate technology

mechanical environment through multiple

simulates native human cardiac environment by

cellular processes. When cultured on overly stiff

using flexible substrates.

substrates like glass or plastic, cardiomyocytes
respond with drastic transcriptional and metabolic• Standard 96-well format

dysregulation. 10 µm thin PDMS membranes• Optional black plates for optical stimulation

integrated into FLEXcyte plates simultaneously• Recordings possible in 2D or 3D cell cultures

provide physiological elasticity of native human• Produced and certified by InnoVitro

heart tissue and strong mechanical support.

Leveraging maturity of iPSC-CM cells by
FLEXcyte 96 assays
Physiological relevanceIn vivo

Ex vivo

FLEXcyte 96

Impedance

Optical methods
Throughput

FLEXcyte 96

Advance your research withcontractile force quantification
Day 24
Day 44

150%
100%

60

145012104083062050%

410200%−0.30.20.700Time (s)Contractile force (mN/mm2)

Day 7

Beat rate (bpm)

Normalized amplitude200%

204060Days in culture

Contractile force measurements

Mature cardiac phenotype

Applications of human induced pluripotent stem

Increase of contractile force and decrease ofcell derived cardiomyocytes (hiPSC-CMs) inbeat rate mirror the maturity levels of hiPSC-

disease modeling, safety and in vitro drug screensCMs. The FLEXcyte 96 provides the necessaryheavily depend on the contractile force readout.

prerequisites to perform sophisticated cardiac

Cells grown on FLEXcyte 96 flexible membranes

research, comparable to the in vivo conditions:demonstrate stronger contractility with time, withphysiological mechanical conditions supporting

no additional stimulation.

mature cell behavior and contractile force(mN/mm2) quantification.

1 µM
100 nM

200%

Control

150%
100%

Normalized amplitude

Normalized amplitude

250%

150%

1 µM
100 nM
Control

100%

50%

50%
0%

0%
−0.2

00.20.4

Time (s)

− 0.4

− 0.2

00.20.4

Time (s)Positive inotropy: Isoproterenol

Positive inotropy: Omecamtiv mecarbil

Beta-adrenergic agonist isoproterenol displays

Positive inotropic effects are present

increased inotropic effects without any

independently on the molecular mechanism

previous stimulation, when the iPSC-derived

used to induce them, as they are also seen whencardiomyocytes grow on flexible substrates.

phase III cardiac myosin activator omecamtiv
mecarbil is applied.

FLEXcyte 96

The FLEXcyte 96 includes• Recording unit with environmental control (optional hypoxia feature)• Recording and analysis software package• Contractile force readout• Automated multichannel pipetting—Integra Viaflo Assist (optional)• SOL optical stimulation (optional)• Various FLEXcyte recording plates• 1 year warranty with further optional comprehensive service plans available• Unmatched application support

Specifications
Readout

Mechanical contraction

Resolution

1 ms

Parallel recording channels

96

Temperature sensing

Multi-sensor temperature control

Validated human induced pluripotent stem cells

Yes

Validated embryonic stems cells

Yes

Pro-maturation environment

YesStand-alone system

Integrated Incubation System

Automated liquid handling

Integra Viaflow Assist

Cardiac cell pacing

Optical (optional)

Plates available

Standard transparent, optical stimulation platesaccelerate your researchnanion Europe
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