The 4th International Conference on Recent
Advancements in Sustainable Management ofLivestock Waste and Rural Environment(March 27 ˗ 28, 2021)

Abstract Book

By Dr. Xiaoyong QIAN

Organizer

Co-organizer

Sponsor

University of Tsukuba

International
Bioprocessing
Association

Institute of Livestockand Grassland Science,
NAROPREFACE
The world population exceeded 7.84 billion people as of January 2021,
which is continuing to grow each day and estimated to reach 9.77 billion
by 2050. Along with the population growth, sustainable food supply isessential. In most developed and developing countries, the resultant rapid
growth of livestock industry has triggered many environmental issues
including water, air, and soil pollutions, mainly attributable to theimproper or even no treatment of livestock waste.
In Japan, the annual production of livestock wastes is relatively stable
at 80 tons during 2015-2020, in which > 80% are dairy and swine wastes.
In addition to the efficient and regulatory policies and management, thegood practices on livestock wastes resulted in a relatively high utilization
ratio of about 87%. However, the livestock industry, especially cattle andpig production, has still been claimed to associate with water pollution,
odor problem, energy and resources conservation issues, greenhousegases (GHGs) emission, etc. in the surrounding rural area.
Thus, more sustainable and advanced technologies for the treatment
and utilization of livestock wastes such as high-efficacy methane
fermentation, carbonization, recovery as organic fertilizer, etc. aredemanded, which can ease the burden of environmental management inrural areas as well. Taking all the above aspects into consideration, notonly more advanced treatment and utilization technologies of waste
and/or wastewater but also more strict policies and regulations are
crucially important for the sustainable management of livestock industry
and the betterment of rural environment.

SCHEDULE
17:00 (JST), March 26 (Friday), 2021
End of Registration
March 27 (Saturday), 2021 ~ March 28 (Sunday), 2021
Conference Days

KEYNOTE SPEECH
Prof. Dr. Kiyonori HAGA
Sustainable Recycling of Livestock Wastes by Composting andEnvironmentally Friendly Control of Wastewater and Odors

Prof. Dr. Renjie DONGThe Treatment and Recycling of Livestock and Poultry Manure and
Its Contribution to GHGs Emission Reduction

Prof. Dr. Huu Hao NGO
Insight into Enzymatic Processing in Animal Waste Management

SPECIAL LECTURE
Prof. Dr. Ashok PANDEY
How to Write a Scientific Manuscript and Get It PublishedROOM CHAIRSMarch 27 (Sat), 10:00 ~ 12:00
Room A: Prof. Kaiqin XU (NIES) & Prof. Weiwei HUANG (HNU)
Room B: Prof. Ikko IHARA (KU) & Prof. Lyujun CHEN (THU)
Room C: Prof. Kazuya SHIMIZU (UT) & Prof. Chaoxiang LIU (CAS)

March 27 (Sat), 14:00 ~ 16:00
Room A: Prof. Yanwen SHEN (SJTU) & Prof. Xinmin ZHAN (NUI)
Room B: Prof. Takeshi MIZUNOYA(UT) & Prof. Donghui WEN (PKU)
Room C: Dr. Miyoko WAKI (NARO) & Prof. Zhenhu HU (HFUT)March 28 (Sun), 10:00 ~ 12:00
Room A: Prof. Wenshan GUO (UTS) & Prof. Wenqiao YUAN (NCSU)
Room B: Prof. Helmut YABAR (UT) & Prof. Gang LUO (FDU)
Room C: Prof. Jiayang CHENG (NCSU) & Prof. Motoo UTSUMI (UT)March 28 (Sun), 14:00 ~ 15:45
Room A: Prof. Duu-Jong LEE (NTU) & Prof. Shicheng ZHANG (FDU)
Room B: Prof. Yaqian ZHAO (XUT) & Dr. Tian YUAN (NU)
Room C: Prof. Weixiang WU (ZJU) & Prof. Zhongfang LEI (UT)CAS: Chinese Academy of Sciences
FDU: Fudan University
HFUT: Hefei University of Technology
HNU: Hainan University
KU: Kobe University
NARO: National Agriculture and Food Research
Organization
NCSU: North Carolina State University
NIES: National Institute for Environmental
Studies

NTU: National Taiwan University
NU: Nagoya University
NUI: National University of Ireland Galway
PKU: Peking University
SJTU: Shanghai Jiaotong University
THU: Tsinghua University
UT: University of Tsukuba
UTS: University of Technology Sydney
XUT: Xi’an University of Technology
ZJU: Zhejiang University

ORGANIZATION COMMITTEE
Chair
Dr. Zhenya ZHANG
Professor, University of Tsukuba, Japan

Co-chairs
Dr. Lyujun CHEN
Professor, Tsinghua University, China

Dr. Yutaka KITAMURA
Professor, University of Tsukuba, Japan

Dr. Duu-Jong LEE
Professor, National Taiwan University, Taiwan

Dr. Ashok PANDEY
Professor, CSIR-Indian Institute of Toxicology Research, India

Dr. Norio SUGIURA
Professor, University of Tsukuba, Japan and University Technology
Malaysia, Malaysia

Members
Dr. Ikko IHARA
Associate Professor, Kobe University, Japan

Dr. Kazuichi ISAKA
Associate Professor, Toyo University, Japan

Dr. Takuro KOBAYASHI
Senior Researcher, National Institute for Environmental Studies, Japan
Dr. Zhongfang LEI
Associate Professor, University of Tsukuba, Japan

Dr. Rui LIU
Professor, Yangtze Delta Region Institute of Tsinghua University, China

Dr. Hideaki MASEDA
Professor, National Institute of Advanced Industrial Science and Technology,
Japan

Dr. Takeshi MIZUNOYAAssociate Professor, University of Tsukuba, Japan

Dr. Ryozo NOGUCHI
Associate Professor, University of Tsukuba, Japan​Dr. Kazuya SHIMIZU
Associate Professor, University of Tsukuba, Japan

Dr. Tatsuo SUMINO
Professor, Toyo University, Japan

Dr. Kazutaka UMETSU
Professor, Obihiro University of Agriculture and Veterinary Medicine, Japan

Dr. Motoo UTSUMI
Professor, University of Tsukuba, Japan

Dr. Miyoko WAKI
Senior Scientist, National Agriculture and Food Research Organization, Japan

Dr. Kaiqin XU
Professor, National Institute for Environmental Studies, Japan

Dr. Helmut YABAR
Associate Professor, University of Tsukuba, Japan

Dr. Xinmin ZHAN
Professor, National University of Ireland Galway, Ireland

SCIENTIFIC COMMITTEE
Dr. Fabrizio Adani
Professor, University of Milan, Italy

Dr. Guanyi CHEN

Professor, Tianjin University, China

Dr. Jiayang (Jay) CHENG

Professor, North Carolina State University, USA

Dr. Xiaohu DAIProfessor, Tongji University, China

Dr. Renjie DONG

Professor, China Agricultural University, China

Dr. Chuanping FENG

Professor, China University of Geosciences, China

Dr. Kensuke FUKUSHI

Emeritus Professor, The University of Tokyo, Japan

Dr. Wenshan GUO

Professor, University of Technology Sydney, Australia

Dr. Zhenhu HU

Professor, Hefei University of Technology, China

Dr. Weiwei HUANG

Associate Professor, Hainan University, China

Dr. Wenli HUANG

Associate Professor, Nankai University, China

Dr. Sang-Hyoun Kim

Associate Professor, Yonsei University, Republic of Korea

Dr. Gopalakrishnan KUMAR

Associate Professor, University of Stavanger, Norway

Dr. Fengting LI

Professor, Tongji University, China

Dr. Fusheng LI

Professor, Gifu University, Japan

Dr. Yu-You LI

Professor, Tohoku University, Japan

Dr. Chaoxiang LIU

Professor, Institute of Urban Environment, Chinese Academy of Sciences, China

Dr. He LIU

Professor, Jiangnan University, China

Dr. Yu LIU

Professor, Nanyang Technological University, Singapore

Dr. Gang LUO

Associate Professor, Fudan University, China

Dr. Huu Hao NGO
Professor, University of Technology Sydney, Australia

Dr. Xiaoyong QIAN

Associate Professor, Shanghai Academy of Environmental Sciences, China

Dr. Genxiang SHEN

Professor, Shanghai Academy of Environmental Sciences, China

Dr. Yanwen SHEN

Professor, Shanghai Jiaotong University, China

Dr. Sang Jun KIM

Professor, Korea University, The Republic of Korea

Dr. Reeta Rani SINGHANIA

Professor, The Centre for Energy and Environmental Sustainability (CEES),
Lucknow, India

Dr. Mohammad J. TAHERZADEH

Professor, University of Borås, Borås, Sweden

Dr. Rajeshwar Dayal TYAGIProfessor, Centre Eau Terre Environnement, INRS, Quebec, Canada

Dr. Chang WANG

Professor, Tianjin University of Science and Technology, China

Dr. Zhiwei WANG

Professor, Tongji University, China

Dr. Donghui WEN

Professor, Peking University, China

Dr. Weixiang WU

Professor, Zhejiang University, China

Dr. Hanqing YU

Professor, University of Science and Technology of China, China

Dr. Tian YUAN

Postdoctoral Fellow, Nagoya University, Japan

Dr. Wenqiao(Wayne) YUAN

Professor, North Carolina State University, USA

Dr. Guozhen ZHANG
Professor, Lanzhou Jiaotong University, China.
Dr. Shicheng ZHANG
Professor, Fudan University, China

Dr. Yaqian ZHAO

Professor, Xi’an University of Technology, China/University College Dublin,
Ireland

Dr. Nanwen ZHUProfessor, Shanghai Jiaotong University, China

SECRETARIAT
Dr. Ziwen ZHAO
University of Tsukuba, Japan

Mr. Ming GAO
University of Tsukuba, JapanMr. Yang SHI
University of Tsukuba, Japan

GENERAL CONTACT
E-mail: livestock2020@outlook.jp
Tel/Fax: +81-298536703

Keynote Speech

1Keynote Speech
K1- Sustainable Recycling of Livestock Wastes by Composting and Environmentally
Friendly Control of Wastewater and Odors〇Kiyonori Haga*
Technical Expert of Livestock Waste Management, Livestock-Industry’s Environmental Improvement
Organization (LEIO), Tokyo 105-0001, Japan*Corresponding author. Email: haga@leio.or.jp

Abstract: Excess amounts of livestock wastes are excreted in limited area and cause pollution
problems in Japan. Comparative studies on livestock waste management among EU, US and Asia
including Japan are surveyed. In Japan, livestock wastes are mostly separated into solid and liquid
matter. Solid matter is converted into organic fertilizer by means of composting. Composting should
be developed into the technology that has low emission, high-quality and low cost. Various odorouscompounds are emitted from the process of livestock waste management. Odor emission fromlivestock farms should be mitigated to protect the local community. High-quality compost can be
widely distributed to cropland to decrease expenditure of chemical fertilizer. Although liquid matter,namely wastewater or slurry, could be applied to cropland of livestock farms such as EU countries,
most of Japanese farms do not have enough cropland to use liquid matter. Particularly pig farms haveno area to spread wastewater, which should be purified to clean water in accordance with the criteria
of water quality and could be discharged into public water area. The most desirable management of
livestock wastes should involve sustainable recycling as compost and environmentally friendly controlof odors and wastewater.
Keywords: Livestock wastes; Compost; Odor; Wastewater2

Keynote SpeechProf. Kiyonori Haga is currently a technical expert of Livestock
Waste Management, Livestock Industry’s Environmental
Improvement Organization (LEIO), Japan. He obtained bothBachelor (1971) and Master (1973) degrees at the Department ofAgricultural Biological Chemistry, Tokyo University of Education,and got his Ph.D. in Agricultural Engineering at University ofTsukuba in 2001. Right after his Master’s program, he as a researcher
worked in the Laboratory of Animal Waste Management, National
Institute of Animal Industry (NIAI), Ministry of Agriculture, Forestry
and Fisheries (MAFF), and became a Senior Scientist in 1983. Prof.
Haga was the Chief in the Laboratory of Animal Waste Recycling,
NIAI, MAFF since 1993. During 2005-2009, he was the Director of
Department of Animal Waste Management, National Institute ofLivestock and Grassland Science (NILGS), National Agriculture andProf. Kiyonori Haga

Food Research Organization (NARO). Besides being as a technicalexpert of livestock waste management, LEIO since 2009,
Prof. Haga has been a visiting professor in Animal Waste
Management, School of Veterinary Medicine, Azabu University
during 2006-2019. Prof. Haga has a strong research interest and richexperiences in animal waste management, and he is also very activein scientific research and academic fields. In addition to more than
50 publications in domestic and international journals, Prof. Haga as
an editor and/or co-editor published 5 books. Prof. Haga delivered
many keynote speeches in international conferences and workshops,
especially in Asian countries, contributing himself to the sustainable
development of livestock industry.

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Keynote Speech
K2- The Treatment and Recycling of Livestock and Poultry Manure and Its Contribution
to GHGs Emission Reduction〇Renjie DONG*
College of Engineering, China Agricultural University, 17 Qinghuadong Road, Haidian, Beijing 100083,
China*Corresponding author. Email: rjdong@cau.edu.cn

Abstract: Climate change mitigation is one of the key global concerns and should become one of the
key global actions. It is statistically concluded that emission is the result of economy development
mainly because of energy consumption. However, the technologies are almost ready to support lowcarbon economy. Unfortunately, by now there are less cost-effective technologies to “capture” carbon
and to generate “negative” carbon to “neutralize” global emission before the temperature reaches the
2 centi-degrees rise. Animal production is the main source of greenhouse gases (GHGs). Fortunately,according to the emission assessment methodology, based on the commonly admitted baseline, theanaerobic treatment of agricultural biowastes, mainly animal manure, can produce “negative” carbon
emission with acceptable cost. Taking China as an example, the rural and agricultural biowastes cause
about 75% of chemical oxygen demand (COD) and total nitrogen (TN) pollution in the waters. The
annual animal manure amounts to about 2.8-3.8 billion tons. If all the manure is treated in biogas plants,
it will have potential 80-110 billion m3 biogas and 200-300 million tons of CO2e negative emission.
Compared to the net annual CO2e emission of 10 billion tons in China in 2019, manure biogas plants
can contribute 2-3% to the carbon neutralization. This talk will focus on manure generation, collection,
biogas technology, GHGs emission accounting methodology and its use in biogas plants assessment.
Keywords: Manure waste management; Biogas; Greenhouse gases emission; Accounting

4Keynote Speech

Dr. Renjie Dong is currently a Professor at the College of Engineering,
China Agricultural University (CAU). He obtained Bachelor (1985) and
Master’s (1988) degrees at Shandong Agricultural University and Beijing
Agricultural Engineering University, respectively. In 1997 Prof. Dong
got his PhD degree at China Agricultural University. Prof. Dong has
spent 32 years in the education, research and technology extension on
Bioenergy and Environment Science and Technology (BEST) sinceProf. Renjie Dong

1988. He is the PI for more than 20 research projects from the ministriesof Science and Technology, Agriculture, Environmental Protection, andsome provinces and municipal cities. He leads the excellent researchteams in both Beijing City and Shandong Province for BEST researchwith two more professors, 14 associate professors and 42 postgraduates.
His research interests include: (1) organic waste to bioenergy andfertilizer, (2) biomass cookstoves testing and certification, (3)constructed wetlands for organic wastewater treatment, (4)environmental impacts and contributions assessment from bioenergy
engineering, and (5) agricultural development in developing countries.
Prof. Dong is very active for academic international collaborations with
developed and developing countries. He has been the RAC (Research
Advisory Council) member of German Bioenergy Research Center(DBFZ) since 2014, and becomes the international co-chair of RAC for
the term of 2020-2024. He is an ISO TC member of ISO 255 (biogas)
and 285 (household stoves). He set up the Great Cycle platform fororganic waste treatment and renewable resources recovery, and set up andhas been the Chairman of Forum of Renewable Energy Promotion in
Developing Countries (FREPDC) since 2001. Moreover, he organized 15
training programs for 300 scientists and technicians from developing
countries, the network for Chinese agricultural universities to support
Africa in agricultural development (AfriCAU) and for a south-south
sustainable stove experts group to promote the clean stove in developing
countries (S4G) were created in 2005 and 2013, respectively. Prof. Dong
has published more than 300 quality articles in international and domestic
journals, and holds more than 20 patents. He won the second NationalPrize of Science and Technology Progress and MoA Second Prize.

5Keynote Speech
K3- Insight into Enzymatic Processing in Animal Waste Management〇Huu Hao Ngo*Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering,
University of Technology Sydney, Sydney, NWS 2007, Australia*Corresponding author. Email: ngohuuhao121@gmail.com

Abstract: Huge amounts of wastes from livestock and poultry industries have aroused environmental
and public health concern worldwide. Proper management technologies are therefore required for
waste reduction and reutilization. Enzymatic processing has been considered an interesting technology
as enzymes are a part of valuable products from animal wastes while playing important roles in theprocess of waste bioconversion. Since animal wastes contain protein keratin, collagen and fat, they canbe used as substrates for the growth of enzyme-producing bacteria to produce enzymes. In addition,
enzymes promote the biodegradation of animal waste to reduce the environmental pollution, andimproves the production of bioenergy and valuable products. This study reviewed the production of
enzymes from animal wastes under the function of specific microorganisms. The feedstock,bioconversion processes and impact factors for the enzyme production were discussed. The applicationof enzymes for animal waste management were also critically reviewed with respect to: (i) utilizationin animal waste degradation, (ii) nutritional value improvement of animal wastes as feed, and (iii)bioenergy production and value-added products.
Keywords: Enzyme; Animal waste; Bioconversion; Bioenergy; Value-added products

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Keynote SpeechProf. Huu Hao NGO (Ngo) is currently a Professor of Environmental
Engineering and serving as Deputy Director of Centre for Technology in Water
and Wastewater and Co-Director of Joint Research Centre for Protective
Infrastructure Technology and Environmental Green Bioprocess, School of
Civil and Environmental Engineering, Faculty of Engineering and Information
Technology, University of Technology Sydney. He has been duly elected as
Fellow of International Water Association (FIWA) and Fellow of theInternational Bioprocessing Association (FIBA), and Chairman of theInternational Bioprocessing Association. Ngo’s main areas of expertise include
advanced biological waste treatment processes, membrane separation
technologies; alternative resources, management and impacts assessment, andsolid waste management. Ngo also focuses on developing green technologies
for sustainable development for resource recovery, water-waste-bioenergy
nexus, and greenhouse gas emission control. Ngo has received several
important national and international awards/honors such as Clarivate Analytics,
Prof. Huu Hao Ngo Web of Science Highly Cited Researcher 2020 in the category of Biology andBiochemistry Category; Clarivate Analytics, Web of Science Highly Cited
Researcher 2019 in Cross Field Category; Mendeley Data top Cited
Researchers in the world, 2020; Top 1 in Australia and Top 3 in the world in thefield of Environmental Engineering (2015-2020) - SciVal (Elsevier 5-year
ranking); Top 1 in Australia and Top 5 in the world in Bioengineering (20152020) - SciVal (Elsevier 5-year ranking); Top 1 in Australia and Top #13 in theworld in Renewable Energy, Sustainability and the Environment (2015-2020)– SciVal (Elsevier 5-year ranking); Leader in Environmental Science 2020
(THE AUSTRALIAN- RESEARCH yearly ranking); Leader in Biotechnology
in Australia, 2019 (THE AUSTRALIAN- RESEARCH yearly ranking) etc.
Ngo has more than 600 publications including 12 books and 35 book chapters,
5 patents with h-index of 76 and citation of > 25,000 (Google, 02/2020). He
has been invited for giving numerous plenary/keynotes and invited talks,seminars, and lecturers in the international conferences as well as theuniversities/research institutions. He has also been Honorary Distinguished
Professor, International Guest/ Chair professor, Visiting Research Fellow inFrance, China, India, Korea, Japan, Taiwan, Thailand, and Vietnam etc. while
being Conference Chair, Section Chair, and Advisory/Organizing/Scientificcommittee member in a number of conferences. Ngo has appointed as Editorof Bioresource Technology, Elsevier, Associate Editor of Science of the Total
Environment, Elsevier, Associate Editor of Journal of Water Process
Engineering and Associate Editor of Heliyon Journal, Elsevier.
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Special Lecture

8Special Lecture
S1- How to Write a Scientific Manuscript and Get It Published〇Ashok Pandey*

Centre for Innovation and Translational Research, CSIR-Indian Institute of Toxicology Research*Corresponding author. Email: ashokpandey1956@gmail.com

Dr. Ashok Pandey is currently the Distinguished Scientist at the Centre forInnovation and Translational Research, CSIR-Indian Institute of Toxicology
Research, Lucknow, India and Executive Director (Honorary) at the Centre
for Energy and Environmental Sustainability - India. Formerly, Prof. Pandey
was the Eminent Scientist at the Center of Innovative and Applied
Bioprocessing, Mohali and Chief Scientist & Head of Biotechnology
Division and Centre for Biofuels at CSIR’s National Institute forInterdisciplinary Science and Technology, Trivandrum. His major researchand technological development interests are industrial & environmental
biotechnology and energy biosciences, focusing on biomass to biofuels &chemicals, waste to wealth & energy, industrial enzymes, and so on. Prof.
Pandey is also the Adjunct/Visiting Professor/Scientist in universities inFrance, Brazil, Canada, China, Korea, South Africa, and Switzerland andseveral universities several in India as well. He has ~1450
Prof. Ashok Pandey publications/communications, including 16 patents, 95 books, >700 papers
and book chapters with h index of 106 and >50,000 citations (Google
Scholar). He has transferred four technologies on industrial enzymes toindustries and has done industrial consultancy for about a dozen projects for
Indian/international industries. Prof. Pandey is the recipient of many national
and international awards and honors, including Highest Cited Researcher(Top 1% in the world), Clarivate Analytics, Web of Science (2020, 2019 &2018), Top Scientist in Biotechnology (No. 1 in India and No. 8 in the world),
Stanford University world ranking (2020), Fellow in World Society of
Sustainable Energy Technologies (2020), Fellow in Indian Chemical Society(2020), etc. In addition, Prof. Pandey is the Founder President of the Biotech
Research Society, India; Founder & International Coordinator ofInternational Forum on Industrial Bioprocesses, France; Chairman of theInternational Society for Energy, Environment & Sustainability; Editor-inchief of Bioresource Technology; Honorary Executive Advisor of Journal ofEnergy and Environmental Sustainability, Journal of Systems Microbiology
and Biomanufacturing), Journal of Environmental Sciences and Engineering,etc. and editorial board member of several international and Indian journals.
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