Complete List of pGWB and R4L1pGWB with organelle-targeted fluorescent protein
Vectorname
pGWB462
pGWB465
GWB465
pGWB468
pGWB471
pGWB485
GWB485
pGWB488
p
pGWB491
R4L1pGWB462
R4L1 GWB465
R4L1pGWB465
R4L1pGWB468
R4L1pGWB471
R4L1 GWB485
R4L1pGWB485
R4L1pGWB488
p
R4L1pGWB491

Bacterial
Backbone selection
pPZP
Spcr
pPZP
PZP
SSpcr
pPZP
Spcr
pPZP
Spcr
pPZP
PZP
S r
Spc
ppPZP
Spc
p r
pPZP
Spcr
pPZP
Spcr
pPZPPZP
SSpcr
pPZP
Spcr
pPZP
Spcr
pPZP
PZP
S r
Spc
ppPZP
Spc
p r
pPZP
Spcr

Gateway cassette
att R1-Cm
R1 Cmr-ccd BB att R2-ER-sGFP-T
R2 ER sGFP TNOS
att R1-Cm
R1 C r-ccdd BB att R2-NLS-sGFP-T
R2 NLS GFP TNOS
att R1
R1-Cm
Cmr-ccd BB-att R2
R2-Px-sGFP-T
Px sGFP TNOS
att R1-Cmr-ccd B-att R2-Mt-sGFP-TNOS
att
tt R1-Cm
R1 C r-ccdd BB att
tt R2-NLS-TagRFP-T
R2 NLS T RFP TNOS
att R1-Cmr-ccd B-att R2-Px-TagRFP-TgNOS
att R1
R1-Cm
Cmr-ccd BB-att R2
R2-Mt-TagRFP-T
Mt TagRFP TNOS
att R4-Cm
R4 Cmr-ccd BB att L1-ER-sGFP-T
L1 ER sGFP TNOS
att R4-Cm
R4 C r-ccdd BB att L1-NLS-sGFP-T
L1 NLS GFP TNOS
att R4R4-Cm
Cmr-ccd BB-att L1
L1-Px-sGFP-T
Px sGFP TNOS
att R4-Cmr-ccd B-att L1-Mt-sGFP-TNOS
atttt R4-Cm
R4 C r-ccdd BB att
tt L1-NLS-TagRFP-T
L1 NLS T gRFP TNOS
att R4-Cmr-ccd B-att L1-Px-TagRFP-TgNOS
att R4R4-Cm
Cmr-ccd BB-att L1
L1-Mt-TagRFP-T
Mt TagRFP TNOS

Marker for plant
PNOS:NPTII (Kmr)PNOS:NPTIINPTII (Km(K r)
PNOS:NPTII (Kmr)PNOS:NPTII (Kmr)PNOS:NPTII (Km(K r)
PNOS:NPTII (Km( r)PNOS:NPTII (Kmr)PNOS:NPTII (Kmr)PNOS:NPTIINPTII (Km(K r)
PNOS:NPTIINPTII (Kmr)PNOS:NPTII (Kmr)PNOS:NPTII (Km(K r)
PNOS:NPTII (Km( r)PNOS:NPTII (Kmr)Typeno pro,
pro C-ER-sGFP
C ER sGFP
no pro, C-NLS-sGFP
C NLS GFP
no pro, CC-Px-sGFP
Px sGFPno pro,pro C-Mt-sGFP
no pro, C-NLS-TagRFP
C NLS T RFP
no pro,p , C-Px-TagRFP
gno pro,pro CC-Mt-TagRFP
Mt TagRFP
no pro,pro C-ER-sGFP
C ER sGFP
no pro, C-NLS-sGFP
C NLS GFP
no pro, CC-Px-sGFP
Px sGFPno pro,pro C-Mt-sGFP
no pro,r C-NLS-TagRFP
C NLS T gRFP
no pro,p , C-Px-TagRFP
gno pro, CC-Mt-TagRFP
Mt TagRFP

Accession
NumberAP018976
AP018977
AP018978AP018979
AP018980
AP018981
AP018982
AP018948
AP018949
AP018950
AP018951
AP018952
AP018953
AP018954

Vectorname
pGWB562
pGWB565
GWB565
pGWB568
pGWB571
pGWB585
GWB585
pGWB588
p
pGWB591
R4L1pGWB562
R4L1 GWB565
R4L1pGWB565
R4L1pGWB568
R4L1pGWB571
R4L1pGWB585
R4L1pGWB588
p
R4L1pGWB591

Bacterial
Backbone selection
pPZP
Spcr
pPZP
PZP
SSpcr
pPZPSpcr
pPZP
Spcr
pPZP
PZP
S r
Spc
ppPZP
Spc
p r
pPZP
Spcr
pPZP
Spcr
pPZP
PZP
SSpcr
pPZP
Spcr
pPZP
Spcr
pPZP
Spcr
ppPZP
Spc
p r
pPZP
Spcr

Gateway cassette
att R1-Cm
R1 Cmr-ccd BB att R2-ER-sGFP-T
R2 ER sGFP TNOS
att R1-Cm
R1 C r-ccdd BB att R2-NLS-sGFP-T
R2 NLS GFP TNOS
att R1
R1-Cm
Cmr-ccd BB-att R2
R2-Px-sGFP-TPx sGFP TNOS
att R1-Cmr-ccd B-att R2-Mt-sGFP-TNOS
atttt R1-Cm
R1 C r-ccdd BB att
tt R2-NLS-TagRFP-T
R2 NLS T RFP TNOS
att R1-Cmr-ccd B-att R2-Px-TagRFP-TgNOS
att R1
R1-Cm
Cmr-ccd BB-att R2
R2-Mt-TagRFP-T
Mt TagRFP TNOS
att R4-Cmr-ccd B-att L1-ER-sGFP-TNOS
att
tt R4-Cm
R4 C r-ccdd BB att
tt L1-NLS-sGFP-T
L1 NLS GFP TNOS
att R4R4-Cm
Cmr-ccd BB-att L1
L1-Px-sGFP-TPx sGFP TNOS
att R4-Cmr-ccd B-att L1-Mt-sGFP-TNOS
att R4-Cm
R4 Cmr-ccd BB att L1-NLS-TagRFP-T
L1 NLS TagRFP TNOS
att R4-Cmr-ccd B-att L1-Px-TagRFP-TgNOS
att R4R4-Cm
Cmr-ccd BB-att L1
L1-Mt-TagRFP-T
Mt TagRFP TNOS

Marker for plant
PNOS:HPT (Hygr)
PNOS:HPT
HPT (Hyg(H r)
PNOS:HPT (Hygr)
PNOS:HPT (Hygr)
PNOS:HPT (Hyg(H r)
PNOS:HPT (Hyg( ygr)PNOS:HPT (Hygr)
PNOS:HPT (Hygr)
PNOS:HPT
HPT (Hyg(H r)
PNOS:HPT
HPT (Hygr)
PNOS:HPT (Hygr)
PNOS:HPT (Hygr)PNOS:HPT (Hygygr)
PNOS:HPT (Hygr)Type
no pro,pro C-ER-sGFP
C ER sGFP
no pro, C-NLS-sGFP
C NLS GFP
no pro, CC-Px-sGFP
Px sGFPno pro,pro C-Mt-sGFP
no pro, C-NLS-TagRFP
C NLS T RFP
no ppro,, C-Px-TagRFP
gno pro, CC-Mt-TagRFP
Mt TagRFP
no pro,pro C-ER-sGFP
no pro, C-NLS-sGFP
C NLS GFP
no pro, CC-Px-sGFP
Px sGFPno pro,pro C-Mt-sGFP
no pro,pro C-NLS-TagRFP
C NLS TagRFP
no ppro, C-Px-TagRFPgno pro, CC-Mt-TagRFP
Mt TagRFP

Accession
NumberAP018983
AP018984
AP018985
AP018986
AP018987
AP018988
AP018989
AP018955
AP018956
AP018957
AP018958
AP018959
AP018960
AP018961

Vectorname
pGWB662
pGWB665
GWB665
pGWB668
pGWB671
pGWB685
pGWB688
p
pGWB691
R4L1pGWB662
R4L1 GWB665
R4L1pGWB665
R4L1pGWB668
R4L1pGWB671
R4L1pGWB685
R4L1pGWB688
p
R4L1pGWB691

Bacterial
Backbone selection
pPZPSpcr
pPZP
PZP
SSpcr
pPZPSpcr
pPZP
Spcr
pPZP
Spcr
ppPZP
Spc
p r
pPZP
Spcr
pPZP
Spcr
pPZP
PZP
S r
Spc
pPZP
Spcr
pPZP
Spcr
pPZP
Spcr
ppPZP
Spc
p r
pPZP
Spcr

Gateway cassette
att R1-Cm
R1 Cmr-ccd BB att R2-ER-sGFP-T
R2 ER sGFP TNOS
att
tt R1-Cm
R1 C r-ccdd BB att
tt R2-NLS-sGFP-T
R2 NLS GFP TNOS
att R1
R1-Cm
Cmr-ccd BB-att R2
R2-Px-sGFP-TPx sGFP TNOS
att R1-Cmr-ccd B-att R2-Mt-sGFP-TNOS
att R1-Cm
R1 Cmr-ccd BB att R2-NLS-TagRFP-T
R2 NLS TagRFP TNOS
att R1-Cmr-ccd B-att R2-Px-TagRFP-TgNOS
att R1
R1-Cm
Cmr-ccd BB-att R2
R2-Mt-TagRFP-T
Mt TagRFP TNOS
att R4-Cmr-ccd B-att L1-ER-sGFP-TNOS
att
tt R4-Cm
R4 C r-ccdd BB att
tt L1-NLS-sGFP-T
L1 NLS GFP TNOS
att R4R4-Cm
Cmr-ccd BB-att L1
L1-Px-sGFP-T
Px sGFP TNOS
att R4-Cmr-ccd B-att L1-Mt-sGFP-TNOS
att R4-Cm
R4 Cmr-ccd BB att L1-NLS-TagRFP-T
L1 NLS TagRFP TNOS
att R4-Cmr-ccd B-att L1-Px-TagRFP-TNOS
att R4R4-CmCmr-ccd BB-att L1
L1-Mt-TagRFP-T
Mt TagRFP TNOS

Marker for plant
PNOS:bar (BASTAr)
PNOS:barb (BASTAr)
PNOS:barbar (BASTAr)
PNOS:bar (BASTAr)
PNOS:bar (BASTAr)r
PNOS:bar (BASTA()PNOS:bar (BASTAr)
PNOS:bar (BASTAr)
PNOS:barb (BASTAr)
PNOS:barbar (BASTAr)
PNOS:bar (BASTAr)
PNOS:bar (BASTAr)r
PNOS:bar (BASTA()PNOS:bar (BASTAr)

Type
no pro,pro C-ER-sGFP
C ER sGFP
no pro, CC-NLS-sGFP
NLS GFPno pro, CC-Px-sGFP
Px sGFPno pro,pro C-Mt-sGFP
no pro,pro C-NLS-TagRFP
C NLS TagRFP
no ppro,, C-Px-TagRFP
gno pro, CC-Mt-TagRFP
Mt TagRFP
no pro,pro C-ER-sGFP
no pro, C-NLS-sGFP
C NLS GFP
no pro, CC-Px-sGFP
Px sGFPno pro,pro C-Mt-sGFP
no pro,pro C-NLS-TagRFP
C NLS TagRFP
no ppro, C-Px-TagRFP
no pro, CC-Mt-TagRFP
Mt TagRFP

Accession
NumberAP018990
AP018991
AP018992
AP018993
AP018994
AP018995
AP018996
AP018962
AP018963
AP018964
AP018965
AP018966
AP018967
AP018968

Vectorname
pGWB762
pGWB765
GWB765
pGWB768
pGWB771
pGWB785
pGWB788
ppGWB791
R4L1pGWB762
R4L1 GWB765
R4L1pGWB765
R4L1pGWB768
R4L1pGWB771
R4L1pGWB785
R4L1pGWB788
R4L1pGWB791

Bacterial
Backbone selection
pPZP
Spcr
pPZP
PZP
S rSpc
pPZP
Spcr
pPZP
SpcrpPZP
Spcr
ppPZP
Spc
p r
pPZP
Spcr
pPZP
Spcr
pPZP
PZP
S r
Spc
pPZP
Spcr
pPZP
Spcr
pPZP
Spcr
pPZP
Spcr
pPZP
Spcr

Gateway cassette
att R1-Cmr-ccd B-att R2-ER-sGFP-TNOS
att
tt R1-Cm
R1 C r-ccdd BB att
tt R2-NLS-sGFP-T
R2 NLS GFP TNOS
att R1
R1-Cm
Cmr-ccd BB-att R2
R2-Px-sGFP-T
Px sGFP TNOS
att R1-Cmr-ccd B-att R2-Mt-sGFP-TNOS
att R1-Cm
R1 Cmr-ccd BB att R2-NLS-TagRFP-T
R2 NLS TagRFP TNOS
att R1-Cmr-ccd B-att R2-Px-TagRFP-TgNOS
att R1
R1-Cm
Cmr-ccd BB-att R2
R2-Mt-TagRFP-T
Mt TagRFP TNOS
att R4-Cmr-ccd B-att L1-ER-sGFP-TNOS
att
tt R4-Cm
R4 C r-ccdd BB att
tt L1-NLS-sGFP-T
L1 NLS GFP TNOS
att R4R4-Cm
Cmr-ccd BB-att L1
L1-Px-sGFP-T
Px sGFP TNOS
att R4-Cmr-ccd B-att L1-Mt-sGFP-TNOS
att R4-Cm
R4 Cmr-ccd BB att L1-NLS-TagRFP-T
L1 NLS TagRFP TNOS
att R4-Cmr-ccd B-att L1-Px-TagRFP-TNOS
att R4R4-Cm
Cmr-ccd BB-att L1
L1-Mt-TagRFP-T
Mt TagRFP TNOS

Marker for plant
TypePNOS:GPT (Tunicamycinr) no pro,
pro C-ER-sGFP
PNOS:GPTGPT (Tunicamycin(T ii r) no pro, C-NLS-sGFP
C NLS GFPPNOS:GPTGPT (Tunicamycinr) no pro, CC-Px-sGFP
Px sGFPPNOS:GPT (Tunicamycinr) no pro,
pro C-Mt-sGFP
PNOS:GPT (Tunicamycinr) no pro,
pro C-NLS-TagRFP
C NLS TagRFPrPNOS:GPT (Tunicamyciny) no ppro, C-Px-TagRFPg
PNOS:GPT (Tunicamycinr) no pro, CC-Mt-TagRFP
Mt TagRFP
PNOS:GPT (Tunicamycinr) no pro,
pro C-ER-sGFP
PNOS:GPTGPT (Tunicamycin(T ii r) no pro, C-NLS-sGFP
C NLS GFPPNOS:GPTGPT (Tunicamycinr) no pro, CC-Px-sGFP
Px sGFPPNOS:GPT (Tunicamycinr) no pro,
pro C-Mt-sGFP
PNOS:GPT (Tunicamycinr) no pro,
pro C-NLS-TagRFP
C NLS TagRFPr
PNOS:GPT (Tunicamycin() no pro, C-Px-TagRFP
PNOS:GPT (Tunicamycinr) no pro, CC-Mt-TagRFP
Mt TagRFP

Accession
NumberAP018997
AP018998
AP018999
AP019000
AP019001
AP019002
AP019003
AP018969
AP018970
AP018971
AP018972
AP018973
AP018974
AP018975