FBC Lett., No. 30 (2009)

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生命化学研究との出会いそれから．．．
東北大学多元物質科学研究所

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群馬大学大学院工学研究科

桒原 正靖

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東北大学多元物質科学研究所

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東京大学大学院薬学研究科

浦野 泰照

九州大学大学院システム生命科学府

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大阪大学大学院理学研究科

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北海道大学電子科学研究所

小寺 一平

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2nd Switzerland-Japan Biomolecular Chemistry Symposium (SJBCS2009)／国際バイオ EXPO 特別講演セッション／第 24 回生体機能関連化学シンポジウム・第 12回バイオテクノロジー部会シンポジウム／FCCA セミナー FCCA グライコサイエン
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1.

A.D. Ellington, J.W. Szostak, Nature, 346, 818 (1990).

2.

C. Tuerk, L. Gold, Science, 249, 505 (1990).

3.

M. Berezovski, M. Musheev, A. Drabovich, S. N. Krylov, J. Am. Chem. Soc., 128, 1410 (2006).

4.

A. Nitsche, A. Kurth, A. Dunkhorst, O. Pänke, H. Sielaff, W. Junge, D. Muth, F. Scheller, W. Stöcklein, C. Dahmen, G. Pauli,
A. Kage, BMC Biotechnol., 7, 48 (2007).

5.

M. Kuwahara, J. Nagashima, M. Hasegawa, T. Tamura, R. Kitagata, K. Hanawa, S. Hososhima, T. Kasamatsu, H. Ozaki, H.
Sawai, Nucleic Acids Res., 34, 5383 (2006).

6.

M. Kuwahara, S. Obika, J. Nagashima, Y. Ohta, Y. Suto, H. Ozaki, H. Sawai, T. Imanishi, Nucleic Acids Res., 36, 4257 (2008).

7.

S. Obika, D. Nanbu, Y. Hari, K. Morio, Y. In, T. Ishida, T. Imanishi, Tetrahedron Lett. 38, 8735 (1997).

8.

M. Kuwahara, H. Takeshima, J. Nagashima, S. Minezaki, H. Ozaki, H. Sawai, Bioorg. Med. Chem., 17, 3782 (2009).

9.

¡Ppæ_Üæ6AF5E7<HMÌË²æ×VÚLã¾¢· 157 (2003).

10.

S. M. Freier, K. H. Altmann, Nucleic Acids Res., 25, 4429 (1997).

11.

M.M. Masud, M. Kuwahara, H. Ozaki, H. Sawai, Bioorg. Med. Chem., 12, 1111 (2004).

12.

A. Shoji, M. Kuwahara, H. Ozaki, H. Sawai, J. Am. Chem. Soc., 129, 1456 (2007).

13.

K. Ohsawa, T. Kasamatsu, J. Nagashima, K. Hanawa, M. Kuwahara, H. Ozaki, H. Sawai, Anal. Sci., 24, 167 (2008).

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Nature 1994, 371, 578-586.
[2] Ishii D.; Kinbara K.; Ishida Y.; Ishii N.; Okochi M.; Yohda M.; Aida T. Nature 2003, 423, 628–632.
[3] Muramatsu S.; Kinbara K.; Taguchi H.; Ishii N.; Aida T. J. Am. Chem. Soc. 2006, 128, 3764–3769.
[4] Shuvendu Biswas S.; Kinbara, K.; Oya N.; Ishii N.; Taguchi, H.; Aida T., J. Am. Chem. Soc. 2009, inpress.

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1. Akwasi Minta et al., J. Biol. Chem., 264, 8171-8178 (1989).
2. Tetsuo Miura et al., J. Am. Chem. Soc., 125, 8666-8671 (2003).
3. Kumi Tanaka et al., J. Am. Chem. Soc., 123, 2530-2536 (2001).
4. Naoki Umezawa et al., Angew. Chem. Int. Ed., 38, 2899-2901 (1999).
5. Ken-ichi Setsukinai et al., J. Biol. Chem., 278, 3170-3175 (2003).
6. Tasuku Ueno et al. J. Am. Chem. Soc., 128, 10640-10641 (2006).
7. Suguru Kenmoku et al., J. Am. Chem. Soc., 129, 7313-7318 (2007).
8. Yuuta Fujikawa et al., J. Am. Chem. Soc., 130, 14533-14543 (2008).


9. Yasuteru Urano et al., J. Am. Chem. Soc., 127, 4888-4894 (2005).
10. Mako Kamiya et al., J. Am. Chem. Soc., 129, 3918-3929 (2007).
11. Tomonori Kobayashi et al., J. Am. Chem. Soc.,129, 6696-6697 (2007).
12.

 , , 381, 44-50 (2002).

13.

 , 37, 370-374 (2005).

14.

 , 57, 699-706 (2006).

15.

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