FBC Lett., No. 51 (2016)

2016  6 

2  

旅と研究

野水 基義

東京薬科大学薬学部

4 

4 糖脂質関連分子の機能および構造解析〜蛍光顕微鏡と質量分析装置を効果的に利用して〜

樺山 一哉

大阪大学大学院理学研究科

9 DNA はどこまで（化学的に）入れ換え可能か？
名古屋大学大学院工学研究科

樫田啓

15   
九州大学大学院工学研究院
大阪大学大学院工学研究科

22 

特任助教

与那嶺 雄介博士前期課程２年

高

靖馳

稲葉

央



イリノイ大学留学体験記
鳥取大学大学院工学系研究科

26   
第 19 回 生命化学研究会、第 10 回 バイオ関連化学シンポジウム、第 43 回 国際核酸
化学シンポジウム（ISNAC 2016）、第 26 回 バイオ・高分子シンポジウム

受賞、異動

編集後記

4

4

8

9

I
4

I

8

6

4
I

1

I
I

I

I
H
4

I

4

I
I

4

9

4

4

4

I

6

6

4

I
9

I

I
I

4

I6

4
4

9
6

-2-

4

I

I
I

4

I9
9

1

4

I

4

4
I

I

4

4

I

4

I

69

2016

-3-

5

´:+PÁÊźťŽĞ Ğ No. 51 (2016 July)(
()

   

kaba@chem.sci.osaka-u.ac.jp1.

ņ ĵō ńĞ PăŅP´Ģ1ńz«ĳŔĺ!ŅĤÌċŁÏÚžÑšřŚżţſĥĬÅŗıŅ¸ŉ[ħĺģĹıŀÌÜ÷ŅıŁŗÀŒĢÌÜ÷ŅĐT%ŗ6xĴĿħœÁÊSńœıŁńŃĽĺŅ
Ĭ1996bĢ

īő20b$ŀĦœģfĢÁÊSŀ6xĳŔĺşŹŠŤźŰŻŢūŅôĐT%žN-śŞŦŹŮŸŚŬŴżſĬĝ¥qŗÃĴĺıŁīőĢŲţńĴĺÁÊŨŽųžħĮľŎ/ĳŔĺſņŷŞŪŢŹŤŷŴūŗĴĺąÍĐT%Ņ6xÁÊŀĦĽĺģ6xĴĿīőpĽĺıŁņ
ĤķĽīĮĽĺŅĻīőÞ#ŀ¥qŗòŊĿŌĺħĥģĹıŀĢUŅēń®dĽĿħĺ´+PažſŅJÁÊyńFÁĳķĿŎőħĢ¥qQŗäĽĺģ¡oŃĬőÒņğģĴīĴŃ
ĬőĢáĮîĩŇLKmĬeħĢrĮîĩŇÇŒ¢ŃÅņĢıŅFÁŅĪĒŀÌÜ÷ĬÏÚŀĮÐŌńà9ŗļĢÌÜ÷ôŅĐT%6xīőÌÜ÷#NŅÛÁÊŉŁ#ĈŗEĴĺģĞ ĹĴĿ.DñÈŅŨŽųŁĴĺŅĬĢĤŷŞŪŢŹŤŷŴū#_Ņ´¯P»uÖĥŁĤÜÙÏÚńĪ
įœŝżşŸśŢūGM3Ņh&íĥŀĦĽĺģ$ØŀņĢĂN[ńŐŒŷŞŪŢŹŤŷŴūŅÂ
Ć+ŁŢŘŹĆ+ŗÏÚńĴĢæ²?ŁĴĿ²ŔĺÏÚFųŪŸŞţŉŅ¿ÛŅE+ĬĢřżŨşŸżŅĂNº²E+ńŐœŎŅŀĦœıŁŗýÊĴĺ(1)ģiØŀņĢřżţŸż~|qŗtøĴĺÜÙÏÚńĪħĿGM3ĬC'ĴĿĪŒĢıŔŗÌÜ÷´6xĐT%ŀªőĶŁřżţŸżŢş
ŬŹĬ=kĶœŁħĨÒŗA8Ĵĺ(2)ģ
Ğ ŃĸĻŕĨƀŁħĨà9ĬŐŒeħŅĬÁÊØŅqŀĦŒĢÅņüŅGM3ĬC'ĶœŁŃĸ

řżţŸż~|qĬøıœŅīŁħĨĠ

ÐŌġńà9ŗĽĺģGM3ņÌÜ÷ŃŅŀÏÚÝŅF

ÝńO>ĶœģGM3ĬřżţŸżŢşŬŹń­ŗľŁĶŔŇĢ½zņřżţŸż3VžIRſŀ
ĦŕĨŁfĺŒŗįĢÌÜ÷ĬgxĶœÜ÷ŷůŪńIRĬĔÉĶœīŗŢŶÌWc*Ąām¤ŀòŊĺģĹŅÒĢřżţŸż~|q°wŅÜÙÏÚńĪħĿIRņŢşŬŹĀBŅŜűśŷī
őíĕĴĿħœıŁŗRïŀĭĺ(3)ģĴīĴĢŋĻóņ¡ĽĿħœģŃĸGM3ĬCĩœŁIRĬŜűś
ŷīőFŔœŅīƀŀĦœģıŅ¹;ńYĴĿņĢ#NŅėĖ»½µŗđĴŃħ¤ŁĴĿ´ÏÚŗĨRĜŅćèqŗvĵĢGM3ĢIRĢŜűśŷŅ^>ŏ)wŗãęlČŏĖNęl
ČŗµħĿíĶœRĜŗäĽĺ(4, 5)ģûŀņĢıŅęlČ{åŗµħĺÌÜ÷ŏÝ^>#NŅ)wíńà9ŗļžŋĺÇŒ¢ĴĿſĢÌÜ÷ŅgxĶœ)»Ńl\ūŵřżŗæ²ŀĭœêŅ{åĬŔŃħīÎŀĦœģŋĺĢÜ÷ŷůŪŅgxńÌÜ÷ŅÜÙĆ¡@Ņčĳŏ£Ć+ŃłŅĚĬćèŀņŃħīŁ×ĩœńßŒĢ÷ĉ#çÕŗµħĺþí(6, 7)ŎäĽ
-4-´:+PÁÊźťŽĞ Ğ No. 51 (2016 July)ĿħœģĞ ŎĨ]Ĵù}eĮľŅõŗýÊŀĭŃħŅīŁ2¾ŎĴľľĢŀOŅ{åŏÀéńŁ
őŖŔŃħÞ¶ŃºsŀĢÏÚÝŀøıĽĿħœřűżŪŗÌÜ÷Ņë­īőìœıŁĬŀĭĺőMĴħŃĢŁħĨŅĬÅŅŀĦœģ2.

ƆǊõƸ:ňƀÑ&ĤżƒĚ ŝ'tƥ&3$Ůų\Eơ&âÁ%ðǇ:w

4ńÁŬŻƀt&ũŽơŭā

recovery$#&ƀQq6"%36ŬŻ*&LFXmƬó&ØƗ:ƙ.

ƚ Ŭ '  & ƒ Ɯ (8) % Ơ 6
Fluorescenceāƹ¾Ñ¯Ú

after



6

1%

photobleachingǐFRAPǑ"¹(76ăĬ!k?_Pm?fr
MqF%36Ñ§ÿƒĚ:Ƈ6FRAPĬ!'QrGSV"$6Ñ%ƆĢƟ:č"

÷Ǝ!6&ĢƟÑ:ŒńŬŻ%

ƒĚǈÄ(ROI)%î

nrKr:ŘĐƻĿÞÑ&Ĥż:Ê"$Ɔ&.:Ʀƃ(_lrR)61ŔœÑv§$4(_lrRǈÄ&Ɔ'½ô$§œ$Ñ$4(ćĉ6"!¸¿&ĖƦƃÑĴ5³_lr

RǈÄ&Ɔ'½ô&ĐƻŮƫ%"1$Ɔ½ôŃ:Fk^%`oSV6

"!ŔœÑ&§œāĩŃ2ćĉƨì:Ŧ6"
Ÿ'GM3ĠāŬŻ:ňƆĢƟIRĬǑŋīƽĬ

!6ǐÀ1Ǒ7-!%Ť

3)caveolin-1&ŇŬŻ?frMqFǐFRAP3)þ¯ùGM3:ňĜģØǍ$#%35ǒGM3&ŒńtĎ%3

IR&J_iYSV&ŬŻƀŕt&lMqĨÆ"GM3&L=mƲĨÆ&ǄǂœŖ|ňÉÎůěIR/ caveolin-1ƍ³ƒǁ6ÑĤĠ:ØƗ

6(4)ǐÀ2Ǒ

 
  



-5-

´:+PÁÊźťŽĞ Ğ No. 51 (2016 July)-FRAP&3$ăĬ'

Ĕň!6"ŷ476®ò4"ƑÜx1ƅĺ&ðǇ~ñ&ƚŬ$ƒĚ%7:ƒĲ!6ť"
c?Eoİ

(ƅĺ%
c?Eoİ

ŬŻƀ&İ§ùË©:ƒĚ6&%űŲ

60ŰƓœUrQ:»ǉļ"$6"ŤŸ'ŷ

!ńÁU[?N:ƑÜŪ%ß6ĞƔ:ƙ.

6

U[?N"'Hq]hrQrÆę&ā$#%ň476õŬ¥åĄƈ!

47Ɗŵ&"!5ªŐåÔ$#&ƴ!įň7c?Eoİ

U[?N"2±&LlqMbq`:ň6ŤŸ4'-YÒÃ&ãİ"¹(762&ÅÂņÈ:´

Đ%ƑÜŪ%50&ĞƔ:ƇÐ0%ŀČ&LlqM%FITC:ĵ¥ãİïā&Śƛ:Ƈ"8ƺÐñū20!ãİ:ƆƑÜ6"!(À3A)ãİ&Ƶœ$Ɲŧ'LlqMbq`&İƵƖ×!l=mQ?e%°Ë!6Ɩ×ËĒ"k?
_?frMqF"&Q?ekF'Ċ
Şşì!6ĕĞƔ"
Rhodamine-C18:ŀČ&İƣ%¶Ĕ1ŀČ&İƵ:2.0 µl/min

45.0 µl/min%tĎ"8Ne
rO%İƣè

ËĒĝƃĳ&ĨÓ$#1Ə47$(À3B)

 Ħ%U[?N%EGFP:Õ×ŒńCHO-K1ŬŻ:ĈŠŀČ&LlqM%Vl`Lq:ĵ¥´ġ&ĞƔ:Ƈ&ůěVl`Lq¶Ĕİƣ!&.ŬŻ

7ġ:l=mQ?e!Śƛ6"(À4A)Ħ%tƕĘ&uƆĢƟ?qNlq¯Ú
Ż%



!

(IR-EGFP):Õ×ŒńŬ

HnNTorm&ƾ¬!6fRm©β-LEoUDNVlqǐMβCDǑĔĽ

&ņÈ:ƑÜŪ%´Đ%5FRAPĬ%36§ÿƒĚ:ƇĂMβCD%đǃ
7ŬŻ%



'7-!%ŤŸ4Ƈ6Ƨç&CkNëU>SLh%FRAPķ×(À4B)"´ġ%Ĕý%IR-EGFP&ƀİ§ù&uƑÜ7(À4C)ėĄƈ'ŬŻƀŽơ&ĤżË©:k?_?frMqF!ƑÜ!6"©

4üƢ76LFXmó:øµ6ċƐƅ&ƘØǍ%&ĔĽ:´Đ%” ƑÜĞƗ6"

!6"ŷ

-6-

6

6

4ŬŻƀÑ&İ§ùËŬŻ*&ƅđǃ´:+PÁÊźťŽĞ Ğ No. 51 (2016 July)3.

ơƵĚƊŵ:ňƀÑ&ĠƩƒĚ ŤŸ4'À2&řŢƜ%
GM3Ƃ

ĻŎùłÿ&ŽźŬŻ!'GM3&Œń}ƪ!'$&ŽźƲŭā%1Ë©

ì¨ƱƭöĬ%3Ň6&!'$

!LjũÛƝƌ76ŬŻƀ&ŊǆįùvĹùŉǐDRMŉǑ%ǀš6

GM3&TOF-MS%36ƒĚ:ƙ.ĕĞƔ%%35ÑƵǈÄ%blcrł&]rE
ǉ

"1ŷ



DRM&Ɲƌ%ň6Triton X-100

ń60%MSƒĚŇ!Ŋǆįù:ƾ¬6ČĬ"ÏÙ76"²ŠBkeprE:¶0

»

ŠĞƔ

ůě1,2-MEooAQqǐDCEǑ%36Įı¦ěœ%Ŋǆįù:ƾ¬!

6":ŒƏ(399.99%&ƾ¬%ā¤)MSƒĚ
°ż$łÿ%$6"(6)&Ũƾ¬Ĭ:ňÖ$Ŋǆįù

ſŏÊħ¾ÑTNF-αŅ"ĖŅ&3T3-L1ŽźŬŻ:DRMŉñĪDCEĮı:ƇTriton X-100:ƾ
¬DRMŉ:&--LC-MSƒĚ
"8ũŽơÌ&ā
%MS&Ğ¼

Í0

ǌº½ư60%

æƳ6"arNk?qZ?O&ðǇƏ47!SvennerholmƱ%3

ğù&Łơ:ƾ¬&LC-MSƒĚ:Ƈ%100 pM 96Đƻ&TNF-αŅ%35ŽźŬŻ&GM3

ū2%É¥6":Ç·ƯĆù'Ə47+,sġ%É¥6"6

ǐÀ5Ǒ ~½ƒĚ3T3-L1ŽźŬŻ!'TNF-αŅ%3
6ůě:òǎŽźǋ:wáōgUmc@NǐKK$$ǎǏŹĸc@NǐC57BL/6JǑ¡ŗyŽź2ǎǏ&ũ

4ŘƷŽźƲǐC162C18Ǒ&¢³

É¥6"

Ķà6"

łÿ!N^>qIŽơ&PkdWƷƹË©

ƚŬ$ÑŠ'Ç·7
Ç·7

6(9)-

Ç·7

ś7

6(10)&3

%Źĸ

6"

4~ñƄǏ&Źĸg

GM3:'0"6ũŽơ&LC-MSƒĚ:Ƈ

"!Źĸ:íƶ"62Ãũáō&ōÿ&4$6ƒď:Ƈ
4.

ŘƷŽźƲǐC16Ǒ:¶/N^>qIdAlqǐSMǑ

É¥ƹƷŽźƲǐC24Ǒ& SM
Umc@N:ň

GM3'ÑŠ%Ƽ942%É¥

3)KKAyǑ&«ä¸¿Žź%ǎǏob/obc@N&ŽźŭŴ%É¥6ƿ%PkdWƷƹ&{×!6ŋŁō Ƥé &Žơƞ%Ý6ªŐƴ&ĭŔì'4%É

6"þ6HnNT

orm&ƞą'18;&"ēƤ!'ũŽơ&Ň³ā:ą5ũŽơÑ
&1&:Ƌ56"!ŇįŶĀō2őžƁ3)ŜŮŌú%Ý6řŢƺŒƪƇnam!&ƉńÃƒĚ2lNE^

6

$

4&ňĤê"

<EQr"

&×ƵƒĚ

ǆt!#&3$Ë©

Ç·76(Ƣ'

5!5tƕÑ:Ġāā"6ŬŻƀƉ6& ǐŬŻƀt&LFXmƬÑ%#&3

-7-

$ðǇ

´:+PÁÊźťŽĞ Ğ No. 51 (2016 July)
:­,&

Ǒ%Ƽ

 ŤŸ4&řŢ)ŬŻƀ¯Ú
ĄƈŬŻƀŽơ&&Čćĉ%

ǅç%Ʈ

ĚĄƈ

'ľ"

ďŚ$Ɯď'ā7
Ūœ$ƒĚĬ

$Śţ6ŬŻƀ&İ§ù

3&ûÇ:Ċ©60%'k?_Pm?frMqF5ŬŻƀĠāŽơ&ĠƩƒĚ%'LC-MS:z£"6²ŠơƵv°ĥ!6"ŷ

6


5.

ôú
Ğ ÁÊŗÿäĶœńĦĺŒĢÌÜ÷ńĎĶœĲ[ŗöŒŋĴĺ,-ÆâÆGP #N´Ý
ÁÊyč

ŭ4

ĢųřŞŻ¦ũŮřţŗĲĘħĺ¨GPaPă <n"Ģ÷ĉ#ńĎĴĿĪðńŃŒŋĴĺGP³aPă Ċ(ĢŃőňńË:ě

GP´:ÆPă \`ZI(ĢRĜŗXńRäĴĿĮŔĺGďGP³PÁÊÆ.DñÈŅ


H7ĢĎ5ń©ĮjÄ·ĴİŋĶģŋĺÁÊŅÓÔŗĲ

ĘħĿħœGďG

P³PÁÊÆ ©¬§Ńőňń©¬ÁţťŧůĢP´Ņ¼ń©Įvô·ĴİŋĶģ
0×±
1.

Kabayama K, Ito N, Honke K, Igarashi Y, & Inokuchi J (2001) Suppression of integrin expression
and tumorigenicity by sulfation of lactosylceramide in 3LL Lewis lung carcinoma cells. J Biol Chem
276(29):26777-26783.

2.

Tagami S, et al. (2002) Ganglioside GM3 participates in the pathological conditions of insulin
resistance. J Biol Chem 277(5):3085-3092.

3.

Kabayama K, et al. (2005) TNF-α-induced insulin resistance in adipocytes as a membrane
microdomain disorder: involvement of ganglioside GM3. Glycobiology 15(1):21-29.

4.

Kabayama K, et al. (2007) Dissociation of the insulin receptor and caveolin-1 complex byganglioside GM3 in the state of insulin resistance. Proc Natl Acad Sci USA 104(34):13678-13683.

5.

Sekimoto J, Kabayama K, Gohara K, & Inokuchi J (2012) Dissociation of the insulin receptor fromcaveolae during TNFalpha-induced insulin resistance and its recovery by D-PDMP. FEBS Lett
586(2):191-195.

6.

Suzuki Y & Kabayama K (2012) Convenient and rapid removal of detergent from glycolipids indetergent-resistant membrane microdomains. J Lipid Res 53(3):599-608.

7.

Kojima H, Suzuki Y, Ito M, & Kabayama K (2015) Structural characterization of neutral
glycosphingolipids from 3T3-L1 Adipocytes. Lipids 50(9):913-917.

8.

Kabayama K (2012) Modulation of growth factor receptors in membrane microdomains. Yakugaku
Zasshi 132(4):417-423.

9.

Tanabe A, et al. (2009) Obesity causes a shift in metabolic flow of gangliosides in adipose tissues.
Biochem Biophys Res Commun 379(2):547-552.

10.

Samad F, Hester KD, Yang G, Hannun YA, & Bielawski J (2006) Altered adipose and plasma
sphingolipid metabolism in obesity: a potential mechanism for cardiovascular and metabolic risk.
Diabetes 55(9):2579-2587.

-8-

×Q>gåëǃƤǇœ œ No. 51 (2016 July) 











 

kashida@nubio.nagoya-u.ac.jpǌǊžŪƆŻ
ŚDNAŢŵ«ƇĹēźĥžšǔśŸÿšƍűƊŗbŤŽžĹƊŭƑǈ]ZnǉƐŦƌŸŞŘDNAŢŵ]ZžŗAžTƐŗGžCƐĴÞŻĝĤũĹĻƐūƌ£ŗůŽŽ÷ƄJƏŭŷžhj>ūƌŘŧŽőŝĴǈàǉžŽ×ő0fŻžĔƊƍźŝĥŷŜƋŗƃŨŻ
DNAŢĵfŷŜƌŸėşƌŘĬ{ŷžŧŽĹĻƐ3ØūƌŧŸŻƉŴŶDNAǈRNAǉž×Ð
gŽŊYƐħşŗƬƮƺƝǅƈƚǅƱƿǇƤŗ0fƯǇƚǇƫŗDNA OrigamiźŹbvŻƏűƌ±¡ŸũŶŽØŢ[NŨƍŶŝƌŘũšũźŢƊ£ŷŗ·ĸ]ZůƍăĨž±¡ŸũŶŒ8ÞźĥŻ
Ƈƃű

kŷŜƌŘ

ũŝŽ

şſ·ĸ]ZŽČ(ĺfEÔžºƆŶŝũŗ(DźŹŽÀāƇźŝǈƇųƎ

Ƒŗ×ÐgÞŻžŧƍƊŽĥžźŤŶÎŷŜƌŢǉŘ5ŗƤǅưƘĥƐåëŨƍŶŝƌ'×ŻŚ·ĸŽåëžƄƑźKŪŻĔşƌśŸėƏƍűŧŸŢŜŴűŢŗŧƍƇŵž·ĸŢǏéōŽ]ZũšűźŝŧŸŻĦTūƌŸƏƍƌŘŢrźŝźƊſ^ƈŭſŝŝŲťŽŧŸŷŜƌŘkŁŗõĀŷÀāūƌ¢ũŝŕwŖ]ZnŽĽ
Ýžzs'×ƐžŪƆŝŤŵšŽåëƙǂǇƴŻƉŴŶĎƏƍŶŠƋŗRomesbergƊž2014{ŻíǎŽ]ZnƐŵ×ÐǈcĂĈǉƐ7ƋŲūŧŸŻ9ũŶŝƌ [1]ŘƃűŗD-riboseƐũŶ]ZƐq)ūƌŧŸŷ
b½źw]ZnŢŧƍƃŷŻ[NŨƍŶŝƌ [2]ŘůƍŻnũŗîþƊŽåëƙǂǇƴŷžŸũŶ±¡ŸũŶŽØŻâßũŗdÎŸžšťłƍű¼İƐŵw·ĸŽĽÝƐĎŴŶţűŘůŽŁŗůƍƊŽ
w·ĸŢdÎ·ĸŽ>gÞÀāƐ¾%ŷţŗšŵdÎŢųşźŝÀāƐŵŧŸƐ¦ƊšŻũűŘ
°êŷžÑŻîþŢĽÝũűŕÜ]ZnŖŻŵŝŶůŽ.mƐ»ğūƌŘźŠŗdÎ·ĸž
D-(deoxy)riboseƐŐ¸ŻƇŵŢŗřž D-threoninolƐũŶŕ]ZŖƐq)ũŶŝƌŘ D-threoninolƐ3ØūƌŧŸŻƉŴŶJŢm§ŸźƋŗƃűD-riboseŷžq)ŢŃũŝ0fŽq)ƇHāŸźƌŘ

ǍǊƒƣñąóƷƒŻƉƌĹĻŽhj> [3]œ îþƊŢĽÝũű«2ŽÜ]ZnŢƒƣñąóǈƽƦǂǃƧƫǉŻƉƌƷƒŷŜƌǈUǌǉŘƽƦǂǃƧƫždÎ]ZnŸÃĩũŶžƌšŻcţź0fƜƓƠƐŵűƆŗ2žŧƍƐƷƒŻũŶũƃŞŸĹĻŢ
hj>ūƌŸ

ũŶŝűŢŗkŁž

ŻDũcţŤĹĻƐhj>ũűŘŧƍžcţźŏţŷŜŴűŘ

ůŧŷŗŧŽhj>ŽēTƐƌűƆŻ NMR ŷ¼İĖ´ƐĎŴű[3b]ŘůŽøµŗƽƦǂǃƧƫžŝĲŝ
Ż0fľŷƟƤƧƘūƌŧŸŻƉƋĹĻƐhj>ũűŧŸŢ0šŴűŘŨƊŻŏŤƁţŧŸŻŧŽƽƦǂǃƧ
ƫK_ŷžĹĻƐhj>ūƌƇŽŽŗƽƦǂǃƧƫŽáĐĶŻdÎ]ZǈƒƩƭǅǉŢŜƌ\JžĹĻƐcţŤhj>ūƌŧŸŢ0šŴűŘŧŽŧŸžƽƦǂǃƧƫŢdÎ]ZŻnũŶàƐ¬ũŶŝƌŧ
-9-

×Q>gåëǃƤǇœ œ No. 51 (2016 July)Uǌœ ƒƣñąóƷƒM¬ DNA Ž¼İĖ´øµŗCƀƽƦǂǃƧƫǈMǉq) DNA ŽĹĻhjŸƐæũŶŝƌŘŧƍƊŽøµšƊŗdÎ·ĸŸ*ŤÚźƌ¼İƐŵ0fŷŜŴŶƇŗdÎ·ĸŽ>gÞÀāǈĹĻhj>ǆàǉƐŵŧŸŢ¦ƊšŸźŴűŘĮŻėşſŗdÎ·ĸŽ>gÞÀāƐ¾%
ūƌŲťŷŜƍſŗŬũƇdÎ·ĸŽ>g¼İƐ¾%ũźŤŶƇƉŝŧŸŻźƌŘůŧŷŗŧŽĚĘƐ3ØūƌŧŸŷŻĭƁƌ½řźÀāƐŴűÜ]ZnƐĽÝũŶţűŘ

UǍǊČ(ƟƓƧƦXƷƒƐ3Øũű·ĸ¹/Ž¾}U

ǎǊČ(ƟƓƧƦXƷƒŻƉƌő|·ĸ¹/ [4]œ dÎ·ĸžºƆŶ&ƍűàƐ¬ũŶŝƌűƆŗáĐĻŽķ1ÑÚÞźĝĤŢHāŷŜƌŘũšũź
ŢƊŗdÎ]ZnžČ(źŹŽŌĊźƝƙƬǂÝ#āƐűźŝŸŝŞÁÍŢŜƌŘůŧŷŗČ(ąóŸÉ(6ŽƷƒƐÜ]ZnŸũŶØŝƌŧŸŻƉŴŶČ(ƟƓƧƦHāźÜ]ZnƐĽÝũűŘ-ÞŻžƃŬČ(ąóŸũŶƷǁǃǅŗÉ(6ŸũŶƒǅƪǀƗƮǅƐØŝűÜ]ZnƐĽÝũűǈUǍǉŘŧŽ
ƉŞźČ(ƟƓƧƦŸŝŞýş£žîþƊŽƕǁƞƬǂŷž ź Ť ŗ Tyagi Ɗ Ż Ɖ Ŵ Ŷ Ľ Ý Ũ ƍ ű Molecular BeaconǈMBǉŻďŨƍƌ·ĸƴǄǇƳŻ¤Ż3ØŨƍŶŝƌ
[5]ŘũšũźŢƊŗ²ŽMBŷžąóŢ¯ìŻøJũŶ

ŝűŽŻnũŗîþƊžŧŽÜ]ZnƐƟƨƼ.ĶŻq)ũűŘŧŞūƌŧŸŷŗ¯ìŽƳǁǇƞǅƙŻƉƌňƐËŷţŗƃűĒ0fŽČ(ąóƐǈĺfEÔƐŨŭƌŧŸźŤǉq)ūƌŧŸƇHāŸźƌŘkŁŗÜ] Z n Ɛ Ǎ Ʒ ƒ q ) ũ ű In Stem Molecular Beacon(ISMB)ž²ŽMBŸÃĩũŶő|źDNAǆRNA¹/ŢHāŷŜƌŧŸƐ¦ƊšŻũű [4a]ŘŨƊŻŗuA'
×ǈ³wcǉƊŢĽÝŨƍűƹǁƺǇǈPLL-g-DexǉŸÜUǎǊISMB ŻƉƌ DNA ¹/øµ- 10 -

×Q>gåëǃƤǇœ œ No. 51 (2016 July)]ZnƐǎ÷q)ũűISMBŸƐØūƌŧŸŻƉŴŶƝƙƬǂǋƯƧƘƙǀƔǅƫÃ500ƐkÕũűǈU
ǎǉ[4c]ŘŧŽĚĘžŽąóŻƇØŷţŗCy3ŸƒƣñąóƷƒƐ3ØũűISMBƇ[NũŶŝƌ[4b, 4e]Řŧ
ƍƊŽƴǄǇƳƐ3ØūƍſVj>ũűõĀ.ŻŠťƌRNAŽHĕ>ŢHāŷŜƌŧŸƇ0šŴű[4e]Ř

ǏǊƖƦƕǅƷƒŻƉƌĹĻŽhj>ǆ(¶¿ [6]œ dÎ]ZnžőÌŷĖłūƌŘŧŽ
ĖłƴǄơƟž×ÀāŻŠŝŶŉŽÀāŷŜƌŢŗ·ĸƐ±¡ŸũŶ3ØūƌŁŻžŬũƇ­ƃũŝĥŷžźŝŘůŧŷŗdÎ]ZnƉƋžƌš
ŻhjźÜ]ZnƐŵŤƍźŝšŸ
ýşűŘDNAžǁǅĸŐ¸ƐŵűƆŗƖƦƕǅ0fǈp-ƽƦǂƟƦǂƯƣǇǂǉƐq)ūƌŧŸŻƉŴŶŅńá
ØƐ3ØũűÜ]ZnƐĽÝũű
ǈU4Aǉ[6a]ŘkŁŻŧŽƖƦƕǅƷƒƐDNAŻq)ũŶƄƌŸŗG-CƷƒƉƋ
ƇhjŷŜƋŗšŵdÎ]ZnŻnũ
Ŷ&ƍűàƐŵŧŸŢ0šŴű
ǈU4BǉŘªŻŗp-ƽƦǂƟƦǂƯƣǇ
ǂžôa(ÏpŻƉŴŶ[2+2](Ö> UǏǊƖƦƕǅƷƒŽ(A)¾}Uŗ(B)dÎ]ZnŻnūƌà
:DūƌŧŸŢãƊƍŶŝƌŘŴ Cƀ(C)(¶¿ŻƉƌĹĻhj>Ř(B)ƖƦƕǅÜ]ŶŗŧŽƖƦƕǅƷƒŻôa(ƐÏ ZǈZǉždÎ]ZŸžhj>ũŗăĨŸžcţŤhj>ūƌŘpūƍſĹĻŽ(¶¿Ţ®ŷţƌŘkŁŻ340nmŽôa(ƐÏpũű
ŸŧƎǐ0.ŻDžiũŗĹĻŢcyŻhj>ūƌŧŸŢ0šŴűǈU4Cǉ[6c]ŘŧŽƉŞŻƖƦƕǅ

ƷƒžőŝàƐųŗšŵ(¶¿āƐŵűƆŻŗŧƍƐ3ØũűƬƮ¼İŽĠđǆhj>Ţ
®ŷţƌŘźŠŗřžªŻŽ(¶¿ƷƒŻŵŝŶƇ¹ęƐıƆŗ«ĬŻźƋHĕ(ÏpŻƉŴŶ¶¿ūƌƷƒƇ[NũŶŝƌ[7]Ř

ǐǊņzŇƷƒŻƉƌńfè=4 [8]œ ·ĸ]ZžÃĩÞ&ƍűńćè=eŷŜƌŸK¨Żŗńćè=ŻƉŴŶbŤŽČ(ąóƐÉ(ūƌŧŸŢãƊƍŶŝƌŘÑŻƙƒƭǅžĸ>ńŢ«ƇŤŗŝÉ(6ŸũŶžűƊŤŧŸŢãƊƍŶŠƋŗ
·ĸƐČ(ǀƶǂūƌŁŽcţźRŋŸźŴŶŝƌŘůŧŷŗńćè=Ɛ4ūƌÜ]ZnƐĽÝūƌ
ŧŸƐýşűŘ-ÞŻžƝƘǄƵƗƜǅĞqǈƓƢƴǄƱǂƝƘǄƵƗƜǅǉƐÜ]ZnŸũŶĴũ
űǈU5AǉŘƝƘǄƵƗƜǅžņzŇ¼İƐųŗπńfƐűźŝűƆŻ<ÔÞŻńfè=Ɛ4ūƌŕùûŖŸũŶÀāūƌŧŸŢ®ŷţƌŘũšũźŢƊŗŧŽƝƘǄƵƗƜǅžįxDNAĹĻƐhj>ūƌŸėƏƍŶŝƌÅóøJĶƈƟƤƧƗǅƙūƌűƆŽĆŎÖƐŴŶŝźŝŘůŧŷŗƃŬNMR¼
İĖ´ƐĎŴűŸŧƎŗŧŽƷƒžņzŇ¼İƐŵŻƇĿƏƊŬĹĻŽ.ĶŻüūƌŧŸŢ0šŴ
- 11 -

×Q>gåëǃƤǇœ œ No. 51 (2016 July)UǐǊņzŇƷƒŽ(A)¾}Uŗ(B)hjCƀ(C)ńfè=ŽĳċŻƉƌƷǁǃǅƞƓƻƫŽőĪ|>ŘűŘªŻŏŤƁţŧŸŻŗŧŽƷƒƐq)ūƍſūƌƂŹĹĻŢƅũƎhj>ūƌŧŸŢ0šŴűǈU5Bǉ[8b]

ŘĜõź¹ęŽøµŗŧŽƷƒžƓƢƴǄƱǂZľŽÛÅáØŻƉŴŶĹĻƐhj>ũŶŝƌŧ

ŸŢ¦ƊšŸźŴűŘŧŽÅóøJǆƟƤƧƗǅƙŻ!ƊźŝĹĻhj>žîþƊƇ*ŤũŶŠƊŬc

ţźŏţŷŜŴűŘªŻŗŧŽƷƒŻŵŝŶůŽŕùûāŖŽě"ƐĎŴűŘ·ĸ]ZŻƉŴŶcţŤÉ(ūƌƷǁǃǅƞƓƻƫŸ·ĸ]ZŸŽľŻņzŇƷƒƐq)ũűŸŧƎŗČ(ĺfEÔŢ?$Ƈ¥ũűǈU
5Cǉ[8a]ŘŧŽŧŸžņzŇƷƒŢČ(ąóŔ·ĸ]ZľŽńfè=Ɛ4ũűŧŸƐæũŶŝƌŘƃűŗŧ
ŽƷƒžůŽŽČ(ąóǈƱǃǅŗǄǇƥƻǅŗƬƓǂǃƧƫźŹǉŽÝ(^cŻ3ØŷţƌŧŸƇ¦ƊšŻũŶŝƌ[8c, 8d]ŘǑǊńćè=ƷƒƐ3ØũűwĹĻĠđ [9]œ dÎ]ZnžÅóøJƐ3ØūƌŧŸŷƵƨǄĴÞź]ZnƐkÕũŶŝƌŘ£ŗîþƊŽÜ
]ZnžƟƤƧƗǅƙáØƈŅńáØŗÛÅáØźŹƐ3ØũŶŝƌűƆZ°ÞŻƸƾĴÞźĝĤũškÕŷţŶŝźšŴűŘůŧŷŗźƑŸš D-threoninolƐ3ØũűĚĘŷƵƨǄĴÞźÜ]ZnƐkÕŷţźŝšŸýşűŘ-ÞŻ3ØũűŽžńćè=ǈƫƬǇǆƒƘơƴƤǇǉáØŷŜƌŘńfƫƬǇŸƒƘơƴƤǇŸƐƷƒŸũŶØŝƍſŗƵƨǄĴÞźáØŢ®ŷţƌŘůŧŷŗ
ƫƬǇŸũŶƱǃǅǈPǉŗƒƘơƴƤǇŸũŶƒǅƪǀƗƮǅǈQǉŻƉƌÜ]ZnƐĽÝũűǈUǑǉŘŧƍƊž
ůƍŰƍČ(ąóŗÉ(6ŸũŶƇÀāūƌűƆŗ]ZnƐČ(`>ŻƉŴŶƾƭƤǇŷţƌŸŝŞ3ÍƇŜƌŘŧŽP-QƷƒƐƃŬDNAŻq)ũűŸŧƎŗDNAĹĻŢcţŤhj>ũŗƸƾƷƒǈP-PŗQ-QƷƒǉƉƋƇhjŷŜƌŧŸŢ0šŴűŘŧƍžńćè=áØƐ3ØũűƵƨǄĴÞź]ZnŢHāŷŜƌŧŸƐæũŶŝƌŘůŧŷÂŻŗdÎ]ZƐq)ũŶŝźŝƱǃǅƕǁƛƺǇŸƒǅƪǀƗƮǅƕǁ

UǑǊƱǃǅŔƒǅƪǀƗƮǅľŽńćè=áØƐ3ØũűwĹĻĠđCƀ+ĺŽčĖ©úŘ- 12 -

×Q>gåëǃƤǇœ œ No. 51 (2016 July)ƛƺǇŻƉƌwĹĻŻŵŝŶ¹ęƐĎŴűŘůŽøµŗUǑŻæūƉŞŻŧŽÜ]Znž@ÒŷƵƨǄĴÞźĒJƐūƌŧŸŢ0šŴűŘ>gĺġÃƐ¹ęũűŸŧƎŗǌǓǌŽĒJŗūźƏųĹĻƐũŶŝƌŧŸŢ0šŴűŘªŻĄOÊŝŧŸŻŗŧŽńćè=ƷƒžºƆŶhjŷŜƌű
ƆŗdÎ·ĸŷžĹĻũşźŝĺŸŝŞºƆŶäŝĻļŷŜŴŶƇƵƨǄĹĻƐūƌŧŸŢƏšŴűŘŧŽƉŞŻńćè=áØƐ3ØūƌŧŸŷºƆŶhjźwĹĻƐĠđŷţƌŧŸƐ
¦ƊšŻŷţűŘ

ǒǊŠƏƋŻœ ŗŧƍƃŷĽÝũŶţűÜ]ZnŽ0fĚĘǆÀāŻŵŝŶð@ŻöũűŘŧƍƊŽåëƐį
ŪŶŤŪƌŧŸžŗŚDNAžaŸ)ƍşHāśŸŝŞŧŸŷŜƌŘƝƘǄƵƗƜǅŽƉŞźzŇŷūƊź
ŝƉŞźƷƒŷƇĹĻƐhj>ūƌŧŸžHāŷŜƌũŗƱǃǅŸƒǅƪǀƗƮǅŽƉŞźÃĩÞ@òźĚĘŷƇƵƨǄĴÞźĹĻƐūƌŧŸžHāŷŜƌŘîþƊŽÜ]ZnždÎŸŶƇŵšź
ŝ>g¼İƐũŶŝƌűƆŗ¨řŚŹŧŢ]ZnźƑŲǔśŸŠIƋǈǔǉƐFťƌŧŸŢŜƌŢŗrźŤŸƇ
ǈ>gÞǉÜ]ZnŸPƁƌǃƶǂŽÀāžŴŶŝƌŽŷžźŝšŸŝŞŽŢîþƊŽýşŷŜƌŘŗŧŽǈŶƇŵšźŝǉÜ]ZnƐªŻūƌŧŸŻƉŴŶƉƋb½ź·ĸ±¡ŢĽÝŷţƌŸ
ýşŶŝƌŘƃűŗŧƍƊŽåëƐįŪŶŚźŮDNAǆRNAžÕWŽ>g¼İŻĉųâŝűŽšǔśŸŝŞ
RŝŻnũŶƇƊšŽSïƐƌŧŸƐßũŶŝţűŝŘ

UǒǊŧƍƃŷĠđũűÜ]ZnŽƃŸƆŘ

ģī
œ °êŷöũűåëžūƁŶLGtcgcgŀwgåëçÐĥ4wgoÇÆåëlŷĎƏƍ
ƃũűŘÇÆÈ

ƐžŪƆƟƤƧƲŽ'×£ŗ,KåëũŶŝűŲŝű'×£ŻģÙũŦƃūŘ

ƃűŗ°µžg×ĢÄŽ~ƃż;8źŤŶžkÕũşźšŴűƇŽŷŜƋŗåëŻƏŴűūƁŶŽ£ŻģŝűũƃūŘ
- 13 -

×Q>gåëǃƤǇœ œ No. 51 (2016 July)
Bý

Ó

[1]

a) I. Hirao, Curr. Opin. Chem. Biol. 2006, 10, 622-627; b) D. A. Malyshev, K. Dhami, T. Lavergne, T. Chen,
N. Dai, J. M. Foster, I. R. Correa, F. E. Romesberg, Nature 2014, 509, 385-388.

[2]

J. Štambaský, M. Hocek, P. Kočovský, Chem. Rev. 2009, 109, 6729-6764.

[3]a) H. Kashida, T. Fujii, H. Asanuma, Org. Biomol. Chem. 2008, 6, 2892-2899; b) T. Fujii, H. Kashida, H.
Asanuma, Chem. Eur. J. 2009, 15, 10092-10102.

[4]a) H. Kashida, T. Takatsu, T. Fujii, K. Sekiguchi, X. Liang, K. Niwa, T. Takase, Y. Yoshida, H. Asanuma,
Angew. Chem. Int. Ed. 2009, 48, 7044-7047; b) Y. Hara, T. Fujii, H. Kashida, K. Sekiguchi, X. Liang, K. Niwa,
T. Takase, Y. Yoshida, H. Asanuma, Angew. Chem. Int. Ed. 2010, 49, 5502-5506; c) H. Asanuma, T. Osawa,
H. Kashida, T. Fujii, X. Liang, K. Niwa, Y. Yoshida, N. Shimada, A. Maruyama, Chem. Commun. 2012, 48,
1760-1762; d) T. Fujii, Y. Hara, T. Osawa, H. Kashida, X. Liang, Y. Yoshida, H. Asanuma, Chem. Eur. J.
2012, 18, 10865-10872; e) H. Kashida, T. Osawa, K. Morimoto, Y. Kamiya, H. Asanuma, Bioorg. Med. Chem.
2015, 23, 1758-1762.

[5]

S. Tyagi, F. R. Kramer, Nat. Biotechnol. 1996, 14, 303-308.

[6]a) H. Kashida, H. Ito, T. Fujii, T. Hayashi, H. Asanuma, J. Am. Chem. Soc. 2009, 131, 9928-9930; b) H.
Kashida, T. Hayashi, T. Fujii, H. Asanuma, Chem. Eur. J. 2011, 17, 2614-2622; c) H. Kashida, T. Doi, T.
Sakakibara, T. Hayashi, H. Asanuma, J. Am. Chem. Soc. 2013, 135, 7960-7966.

[7]a) T. Doi, H. Kashida, H. Asanuma, Org. Biomol. Chem. 2015, 13, 4430-4437; b) T. Doi, H. Kawai, K.
Murayama, H. Kashida, H. Asanuma, Chem. Eur. J., in press.

[8]a) H. Kashida, K. Sekiguchi, H. Asanuma, Chem. Eur. J. 2010, 16, 11554-11557; b) H. Kashida, K. Sekiguchi,
N. Higashiyama, T. Kato, H. Asanuma, Org. Biomol. Chem. 2011, 9, 8313-8320; c) H. Kashida, H. Asanuma,
Phys. Chem. Chem. Phys. 2012, 14, 7196-7204; d) H. Kashida, N. Higashiyama, T. Kato, H. Asanuma, Bioorg.
Med. Chem. 2013, 21, 6191-6197.

[9]

T. Doi, T. Sakakibara, H. Kashida, Y. Araki, T. Wada, H. Asanuma, Chem. Eur. J. 2015, 21, 15974-15980.

- 14 -

ßD4bíøǕƶǙŨ Ũ No. 51 (2016 July)ŅqŨ ŗŨ ǚƚƋƔƍŨ ƙűŽŸǛ

r[b[bœŨ sbíøœ4bsbņŎŨ Ù0¨Ũ ǚImPACT íøEǛyonamine@chem-eng.kyushu-u.ac.jpŹƎƏßD4bíøǕƶǙŭÊƌƋƅƂīªŮƎPúƎÅơŲƇŝŶŪ[Y2010~ƌ¸»ƌŰƓŽūòƏ

s¿[b[bœŨ ßDÞsbíøóƌƇbơ;ŪƩǓǆƧǔƿƣ[bƣǙǂƥǗ¼

4bóŪǋưƪǗ[bÈbņŪØĭǘ·«íøÅÁŨ į&`ǑƿƹƼƌƇ6XíøEơăƂŪ2016~1´ƚƜŪr[b[bœŨ sbíøœ4bsbņŎŨ Ðǘ¯ŉíøfƌƇÙ0¨ƉżƇëĒżƇųƜ

ƓŽūÝLƏŪŜ­èíøŐæĽǈǖƭǒǌǚImPACTǛŪŭƴǕǗƻƤǅƺƤƎģâè-%ŮƎíøEƉż
ƇŪǂƥƨ×«ơ1ÜèƌßàŽƝĖšƎƲƬǓǙƿǗƭÍƎŐæơęƅƇųƜƓŽūŨ òƏŪßŤ&`ơkŎƌżƇųƜƓŽŵŪ©5nm©5µmƎþuƋÅč·«ƌƗŪēCơƗƅƇ
ųƜƓŽūĶ~ŪđxĂĈ4ƘDNAƾǀƺƬǀǖƱǙŪMOFƋƊƎbottom-upĚƉŪǆƧƼǓƵƭǒǆƤǙƘ
ǜDǈǓǗƼŪMEMSƉŰƅƂtop-downƎĚŵŪƊƄƛƗ$ÖżŪĤƎƮƥƳŞOƌ*ŀżƇŰƓŽūƀ
ƎƚűƋĚèċ±ƎŪÂŬƋ&ŉƎíøŵĘ?ż_ƖƇŪÛ-èƋíøŵßƓƞƇŰƝ7ĬŵůƜƓ
ŽūƓƂŪ>öŠŌƈ<Ġ4ƈŶŪéèƌśçŻŵƠƝÔƗť.ƎƆƃƉŰƓŽū¶÷ƈƏŪƀƎƚűƋŭǁƥǇǓƹƽ4ŮŵĽężƇŰƝíøŞOƎīªơ4SŪźāŻſƇŝŶƓŽū

Programmable motion of DNA origami mechanisms
A. E. Marras, L. Zhou, H.-J. Su, and C. E. Castro, Proc. Natl. Acad. Sci. USA, 2015, 112, 713–718.
Ũ DNAƨǓƪǋĚơáŰƝŹƉƈŪ100 nmõƎþgƋÁĻŵĞ<čƈůƝūĕĊƛƏvĠèƋF
¾ƎÁĻơRƌżƇŪǃǗƱƘƲǒƥƽÁĻƎ<2ņơƅƂDNAƨǓƪǋơƻƯƥǗżŪƀƞƛơĂƔ
?ƠſƇ2ÇèŪ3Çèƌ2ŴŽŹƉŵƈŶƝÅÁơñżƂūŨ ĕĊƛƏŪDNAǂǗƽǔÁĻơǄǔƻƤǗƭǑƿƹƼƉżƇŪąņƌºıƋ¶ŋDNAņơáŰƝŹƉƈŪǃǗƱÁĻơżƂǚI1aǛūƓƂŪûÚƎÁĻƌŪĳÚƎƗƎơwżĵƕŹƉƈŪƲǒƥƽÅÁƗĞżƂǚI1bǛūŹƞƛƎTEMơġhżƂĄ¹ŪǃǗƱĢƏ11-147ũǚ}N79.7°ǛƎőƈ&zżŪƲǒƥ
ƷǙƎĉô2Ə
29.2 nm Ǝ | ƈ & zżƇŰƂūŹƞƛƎÁ
ĻơĂƔ?ƠſƝŹ
ƉƈŪ2Çèƌ2ŷƬǒǗƬ-ƲǒƥƷǙÅ
ÁƗĞżƂǚI1cǛū
TEM  Ŵ ƛ Ū Ģ  Ɖĉô2ƎŒơǈǖƹƼżƂŪÞīǚećǛƉƚŷĒżƇ

I 1 DNA ƨǓƪǋŴƛƝÅÁƎÃIƉ TEM Iū(a) ǃǗƱÅÁū(b) ƲǒƥƽÅÁū(c) ƬǒǗƬ-ƲǒƥƷǙÅÁū(d, e) Bennett ÅÁūīªƚƜýǘņ¦Yū- 15 -

ßD4bíøǕƶǙŨ Ũ No. 51 (2016 July)
ŰƂū³ƌŪ3ÇèƋ2ŶơęűBennettÅÁơĞżǚI1d, eǛŪĝ0DNAơÓ/ŽƝŹƉƈŪŐŏŵ
Ţ2ƈŶƝŹƉơñżƂū

Programmed switching of single polymer conformation on DNA origami
A. Krissanaprasit, M. Madsen, J. B. Knudsen, D. Gudnason, W. Surareungchai, V. Birkedal, and K. V.
Gothelf, ACS Nano 2016, 10, 2243−2250.
Ũ ĕĊƛƏŪ¶ŋDNAơŋƌƆµÅ"ǉǓǊǙơìDNAƨǓƪǋƌJd

4żŪĝ0èƋDNAŋơÓ/ŽƝŹƉƈŪ=ǘtÚƎ<ķèƋ'Ɯ²ŲơęƅƂ
ǚI2Ǜū
Ũ ƓžŪ"

ǉǓǊǙǚAPPVǛơƌJd

4żŪŋƎËňRŴƛ½ň9URơōżƂūƀƎŪǓǗƩǙņơ'¬żƇŪ
DNA-APPVơľŘŻſþĞżƂǚI2aǛū®ŪìDNAƨǓƪǋƌƏŪ\ņƎùŴƛĉƓƈŪǉǓǊǙŋDNAƉ
êĝèƋ¶ŋDNAŵƐƇųƜŪÒ?Ž
ƝŹƉƈǉǓǊǙŵJd4ŻƞƝǚI2bĆĔŇ(ǝAFMƗñŽǛūDNAƨǓƪǋƌƏŪƀƎƌ=ǚI2bŚGĢǛƘtǚI2bŦGĢǛÚƈJdŽƝŭDNAƼǒƹƬŮƗaL
żƇŰƝŵŪƀƞƁƞĆĔƎŇ(ƉƏäƋƝū
ŹŹƑ=ƪƥƽŋǚRgDNAǝŚĔŇ(ƉĆĔŇ(ơƆǛơÓ/ŽƝƉǚI2cǛŪŚG
ĢƼǒƹƬƌĄ?żŪĆĔŇ(ơ¡ñŽƝƂƖŪƀŹƑǉǓǊǙŵ=żƂÚƈJd4ŻƞƝū@ÂƌtƪƥƽŋǚLgDNA;ŧ

I 2 (a) DNA ơŋƌƆ" ǉǓǊǙƎ?ū (b) ǉǓǊǙƎ DNA ƨǓƪǋƑƎJdū (c) tÚŪ(d)=ÚƈƎJdū (e) =ǘtƎ<ķèƋ'Ɯ²ŲūīªƚƜýǘņ¦YūĔŇ(ơƆǛơÓ/ŽƝƉŪtÚƈJd4ŻƞƂǚI2dǛūDNAƼǒƹƬŴƛǉǓǊǙơŶ,ŵŽŪŔ9DNAǚRǞDNAŪůƝŰƏLrDNAǛƉ>ƪƥƽŋơ@°ƌÓ/ŽƝŹƉƈŪ=ǘtƎÚơ'Ɯ²ŲƂǚI2eǛūǉǓǊǙŵė

ơµŽƝŹƉơ)áżƇŪ2öƎƣƬƴǈƶǙĔÿơDNAƨǓƪǋƌŇĉżŪFRETƌƚƝ=ǘtÚơĪƒƝŹƉƈǚI
ƎĮǘĆƎ$ǛŪļąƈ6HƓƈ'Ɯ²Ų<čƈůƝŹƉŵ&ŴƅƂū

3D printed microtransporters: Compound micromachines for spatiotemporally controlled delivery oftherapeutic agents
T.-Y. Huang, M. S. Sakar, A. Mao, A. J. Petruska, F. Qiu, X.-B. Chen, S. Kennedy, D. Mooney, and B. J.
- 16 -

ßD4bíøǕƶǙŨ Ũ No. 51 (2016 July)
Nelson; Adv. Mater. 2015, 27, 6644–6650.
Ũ ĕĊƛƏŪ©µmƎØĭƎVŪĿ£Ū§%ơŪðTƌƚƜ+ƈŶƝǊƥƬǖƼǒǗƲǉǙƶǙơĞż
Ƃū
Ũ

î4ÄĎƌ

iżƇŪ

`

B:ƌƚƝǕǙƯǙéâÍ ǚ direct laser
writingǛơęűŹ
ƉƌƚƜŪƮǇǊ
ƥƬǖǍǙƶǙƎ
ĉþƈÁĻ
ơƈŶƝū
ĕĊƛƏŪƲƬǓ

I 3 (a) ǊƥƬǖƼǒǗƲǉǙƶǙƎÃIū(b) ðãƌƚƝVƉ§%ū(c) ƩǈƴǔÁĻ()ŪưǓǗƱÁĻ ()ū (d) mƛſƢÁĻƎ§%ƉŪ&pżƂĀüƑƎĽūīªƚƜýǘņ¦YūǐǙơƆưǏǆƼƉŪưǓǗƷǙŴƛÁŻƞƝǊƥƬǖƼǒǗƲǉǙƶǙơĥģżƂǚI3aǛūưǏǆƼņƎ
ƔơðŊoƈěğżŪZņŴƛƎðãƈHĲŻſƝŹƉƈĽ.ơæßżŪŻƛƌŪưǓǗƷǙ#ƑǊƥƬǖǄǙƳơj!ŽƝŹƉŵƈŶƂǚI3bǛū®ŪķHĲƌŽƝƉŪ#3ØƎ§%ŵ<čƈůƅƂū@ÂƎÍƈŪƫǏƹǈŵ;ƜZſƝƩǈƴǔÁĻƘŪżĵƔ<čƋưǓǗƱÁĻƗĞƈŶƂǚI3cǛūŻƛƌŪ
ƲƬǓǐǙƎy®AơķƌżƂŪmƛſƢÁĻơǊƥƬǖƼǒǗƲǉǙƶǙƑj!żƇųŶǚI3dǛŪĀŰÏİ
Ɠƈ*ŀżƂŖƌŪðTơķHĲŻſƇŪmƛſƢÁĻƎ§%ƉŪĽơ@°ƌĨlŽƝŹƉŵƈŶƂū

Cellular cargo delivery: Toward assisted fertilization by sperm-carrying micromotors
M. Medina-Sańchez, L. Schwarz, A. K.Meyer, F. Hebenstreit, and O. G. Schmidt; Nano Lett. 2016, 16,555−561.
Ũ ĕĊƛƏŪĿ2ơÆ]żƂþ
`ĀČƌŪðTƈ¥<čƋǊƥƬǖǎǙƶǙơ¤ĴżŪ8ÉĀČ
ƑƉĿ£żƂǚI4aǛūŨ ĕ Ċ ƛ Ə  Ĥ Ǝ Adv. Mater.
2015ƎīªƉšƎÍƈǊƥƬ
ǖƛſƢÁĻơĞżŪƛſƢƎǅ
ƹƸ©ƘŢ2ŽƝ2©ơ³ł4
żƇŪQMƈĹ{ƎƦưþ`Ā

I 4 (a) íøƎÀIū(b) (i)ń`ơżƂþ`ĀČŪ (ii)¢żƂĀČū (c) ǊƥƬǖƛſƢÁĻƌƚƝþ`ĀČƎ(i)Ū(ii)Ŀ£Ū(iii)ďŘū!IƏðã+ƎĜĉūīªƚƜýǘņ¦Yū

ČƉ@ÂƎĺǚ³[70 µm/sǛƈƎŁŕ¥ơŀżƂū2Ǝþ`ĀČƎŴƛŪÑĸKĐĦţǚHOSǝń`ƎơïħŽƝǛƌƚƜŪnņŵƩǙǔżƂƗƎơŃƐǚI4biǛŪǊƥƬǖƛſƢơĹżƇ
ǚI4ciǛƀƎĿ2ơđLƌ+żƂǚI4ciiǛū8ÉĀČWƓƈĿ£żƂŪðTơķHĲżƇŪŶ;ƝŹƉƗƈŶƂǚI4ciiiǛū­ƂƋ^ÌåÍƑƎ<čơ- 17 -

żƇŰƝū

ßD4bíøǕƶǙŨ Ũ No. 51 (2016 July)Ũ Top-down®ƈĞŻƞƂŪÕÅ4?ØƎŭǊƥƬǖǊưǗŮƏŪðãǘřãƈ.ƌŢ2<čƈůƝū
DNAƨǓƪǋĚƏŪƚƜĀƌÁĻơĥģƈŶƝŵŪØÞèƋ.ƈŢ2ŻſƝƌƏcdƎAŵĩşƈůƟűū®Ūß&`ƉƎł?ŵŤŰÔơÎŴżŪßŒļĚƑƎáƌƏµ)ƈůƝƉűū

- 18 -

-BŖĻŚñ ñ No. 51 (2016 July)

BŖĻŚôĔēčĊÂiõęĕ6|ĦùĐ÷Ċċýò?<wĔXčĐïñ èîñ śGao JingchiŜ
?á?B?BãJBñ !;qÀ 2 L
gao@molpro.mls.eng.osaka-u.ac.jpāĕNĖò-úĢĚąóĖ2?á?BJBò¯3.gcñ ĕaGĕĞĒòĻŘŇıÅJB]Ħ÷ĐĻĲĻŘŇıÅĒ(\±ŋŗŚŊĦ÷ģ¢©{ĻŘŇıÅĕ±{Ã^´ĕÞĦ³čĐúĢĚ
ąó¢©±ĪŒŚķŘĲ^´Ė-Äğ@ĕbĦýĊ¢©đºąģāĒĦ'ªĔăò
-BÛĔú÷ĐéKĔÚ·ē]đąórđĖòCĕ-Äř@Ħ'¹ąģ^´ĕÞĔĎ÷ĐòoÌµĂĤĊÂiĕûġò28%ĢĀĐ£÷ĊăĚąó
Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow
Ma, H.; Tu, L. C.; Naseri, A.; Huisman, M.; Zhang. S.; Grunwald, D.; Pederson, T. Nat Biotechnol. 2016, 34,
528−530.
CRISPR/Cas9ĶĸľőĖņıľŔĨĔú÷Đ¸ĂĤĐûġòĈĕ-BęĕWüìÉĔMüĢò-BĔéKĔpē]ĒăĐĦäĝĐ÷ĚąóCRISPR/Cas9ĶĸľőĖĳŅő¦ä^´ĒăĐĂĤĐ÷ģĒ)nĔò
āĕĶĸľőĦ÷ĐCĕ×@OĦ¢©

đ{Ãąģ½ěĞēĂĤĐ÷ĚąóŃıŖĨŚĺYĦ`Ċē÷Cas9ĕ<śdCas9Ŝ ĖòĮĪŁ
RNAśSingle-guide RNA, sgRNAŜĦăĐ{×@Ħ¿ÃăòjąģāĒēþ¥(ăĚąóāĕ
Ċĝò¢©Ĕ±ĻŘŇıÅĦ²(ĂćĊdCas9ĦĂćòsgRNAĦ÷ĐdCas9Ħ{×Ĕ¥(ĂćģāĒđĒąģ×@@OĦ±{

ÃąģāĒüđýĚąóăûăēüġòāČġĕ]Ħ÷Đ¶hĕ×

@OĦ)nĔ{ÃąģāĒü

0æđąó
rÂiđ°¨ġĖCRISPR/Cas9Ĕ7ď÷Ċ×@OĦ¢©đ{ÃąģkĊē]ò
CRISPRainbowĦ8+ăĚăĊóāĕ]ĖRtĒ
Ô÷ò±ĻŘŇıÅĦddCas9Ĕ²(Ăćģĕ@Oĕ¿Ã

1. CRISPRainbow ĔĠģ×ĔĕěăĚąóĈăĐòsgRNAĔ~ēŌĨŉ

Ś{Ãś"ÂiĠĢØf<ŜđĖēþòsgRNAĒdCas9Ħ{×- 19 -@OĕŏŕĽĭœ

BŖĻŚñ ñ No. 51 (2016 July)-ŘzÐĦ¤ěËěòĈĕŌĨŉŘzÐĔĔ¥(ąģĻŘŇıÅĒ±ĻŘŇıÅĕ²(ĻŘŇıÅ
ĔĠĢsgRNA/dCas9¶(Ħ{ÃąģāĒđ{×@OĦ±{ÃăĚąó

rÂiđ°¨ġĖòsgRNAĔ¤ěËĜŌĨŉŘÙĒăĐMS2òPP7ĒboxBĦÖ_ăòĈăĐòMS2Ĕ¥(ąģĻŘŇıÅŁŒĪŘMCPĔBFPĦòPP7Ĕ¥(ąģPCPŁŒĪŘĔGFPĦòĈăĐboxBĦ¿Ãąģ
N22ŎŋĽŁĔRFPĦ²(ĂćĚăĊś11Ŝóāĕ]ĕUĒăĐò1ĕsgRNAĔ¶hĕŌĨŉŘÙĦ¤ěËěòŌĨŉŘĕ¤ě(ĥćĔĠĢŏŕĽĭœŚđ¶hĕ×@OĦ)nĔ±{ÃąģāĒü'ªđąó11ĔąĠøĔòsgRNAĕ¤ě(ĥćĔĠĢo?7ĕ×@OĦēģ®ĕ±đ)n{ÃąģāĒüđýĚąóĂġĔòĞøĎĕÌÆ=ë5ĕ±ĻŘŇıÅĦ÷ĤėòÂCRISPRainbow÷Đ)nĔ{Ãđýģ×

@OĕhĖ15Ĕ:ùĚąó}Ĕ°¨ġĖCRISPRainbowĦ÷Đò¢©ĪŒŚķŘĲĦ³÷ĚăĊó¢©xĕľŗŒĨ
Ēòēģv®Ĕöģ¶hĕ×@OĦ)

nĔ{ÃąģĊĝĔòāĤġĕ×

@OĦ¿ÃąģsgRNAĔēģ¤ě(ĥćĕŌĨŉŘĦ¤ěËěòĈĤĉĤĕŌĨŉŘĦ¿ÃąģĻŘŇı
ÅĒòēģ®ĕ±ĻŘŇıÅĕ²(ĻŘŇıÅòdCas9#ĘsgRNAĦU2OS¢©ĔĂćò¶hĕv®ĕ[Ħ)nĔÍÈąģāĒĔĔ
\ăĚăĊś

2órÂiđ8+ăĊCRISPRainbowĖòRtĕ
dCas9Ĕ±ĻŘŇıÅĦ²(Ăćģ]ĒÔø
2. CRISPRainbow ĔĠģ×"đò¶hĕ±ĻŘŇıÅĦ÷Đ¶hĕ×@OĦ)nĔ{ÃąģāĒüđýò­,þ@OĕŏŕĽĭœ

Ś{Ãś"ÂiĠĢØf<ŜZĄĚăĊó
ñ
Visualization of protein−specific glycosylation inside living cells
Doll, F.; Buntz, A.; Späte A. K.; Schart, V. F.; Timper, A.; Schrimpf, W.; Hauck, C. R.; Zumbusch, A.;
Wittmann, V. Angew. Chem. Int. Ed. 2016, 55, 2262−2266.
ñ ĻŘŇıÅĖ§¼QíĦ&ÿģāĒĔĠĢĈĕ|ªüTĂĤĐ÷ĚąóēûđĞò

ÝśGlycosylationŜĖÚ·ē§¼QíĕĎĒăĐġĤĐ÷Ěąóăûăēüġò28+ĂĤĐ÷ģÝĦ'¹ąģ]đĖ¢©µêĕĻŘŇıÅăû'¹đýĚćħóñ rÂiđ°¨ġĖò¢©Ĕúÿģ{ĻŘŇıÅĕĹŔŘğŀŖĬłŘ7ĕňŁŗįĶ7ĔíĂĤĊNŝĨĹĽŕĲŕĴĵŐŘśO-GlcNAcŜĦ'¹

ąģ]Ħ8+ăĚăĊś13Ŝóāĕ]đĖò{ĻŘŇıÅśProtein of interest, POIŜĒ±ĻŘŇıÅEGFPĕ²(ĻŘŇıÅĦ¢©ĔĂćòĈăĐò
ŒĽŕŋŗŎŘĔĠčĐíĂĤò¢©«ÏÓYĕĊĝĔĨĹĽŕ

ĂĤĊĲŕĴĵŐŘ

śAc4GlcNCyocŜĦăĚąó}ĔòľŀœķŘĒ¥(ăĊ±®¡ĦąģĒò{ĻŘŇıÅĔ%Ģ
ËĚĤĊAc4GlcNCyocĔÎç@·Á4ĿĩŚŕĸřĨŕļŚ$WśInverse-electron-demand Diels-Alderreaction, DAinvŜĔĠĢ±®¡üăò{ĻŘŇıÅĔ²(ăĊEGFPĕßĔ±ðīńŕİŚśFRETŜüÇāĢĚąóEGFPûġ±®¡ęĕīńŕİŚüÇāģĒEGFPĕ±F-üHąģ- 20 -

-BŖĻŚñ ñ No. 51 (2016 July)ĊĝòFRETĕyĔĖ±F-ĪŒ Ś ķ Ř Ĳ ś Fluorescence lifetime
imaging microscopy, FLIMŜüĂĤĐ÷ĚąóFLIMĦ÷ģāĒĔ
ĠĢò¢©ĔÓÜĕÒå±®
¡ĕA2đĞ@FRETĦĴŘ
ŀœĸŀĠþyđýĚąóĚĊò°¨ġ Ė EGFP ü Ac4GlcNCyoc Ĕ Ġ Ģ íĂĤē÷āĒòĚĊòŒĽŕŋŗŎ
ŘĦ*Ěē÷ĨĹĽŕĲŕĴĵŐŘ
śAc4GlcNAcŜĦ÷ģ9(Ĕ±®¡ĦăĐĞFRETüÇāġē÷
āĒĦăĚăĊóāĕĊĝòāĕ]Ħ÷Đ{ĻŘŇıÅĕ

ÝĦ¢©đĔĪŒŚķŘ
ĲąģāĒü'ªđąó°¨ġü/ÞăĊ]ĕ
es

ĒăĐò

ÝíĵĪŀĒEGFPĕÌdòĈăĐï÷

ÝíŖōŕüV·đąó°¨ġĖāĤġ
ĕes

3.





ś"ÂiĠĢØf<ŜĦĊą¢©ĻŘŇıÅFoxo1òp53ĒAkt1ĕÝĕ¢©ĪŒŚķŘĲĔ\ăĚăĊś14ŜóāĤġĕĻŘŇıÅ
ĕÝíĒăĐO-GlcNAcĕěü8+ĂĤĐ÷ĚąóāĤġĕĻ

ŘŇıÅĈĤĉĤĕEGFPĒĕ²(ĻŘŇıÅĦHEK293T¢©Ĕ  Ă ć ò Ac4GlcNCyoc Ē ò ľ ŀ œ ķ Ř Ĕ Ñ ¥ ă Ċ ±  ® ¡TAMRA-TzĦăòFLIM-FRETºDĦ³÷ĚăĊóĈĕ¥uòāĤġĕĻŘŇıÅĕ

ÝĔĠģFRETüEGFPĕ±F-ĕHĒăĐºDĂĤĚăĊólòĶıŗŋŗŎŘĦ`Ċē÷
Ac4GlcNAcĦ÷ģnĔ®¡üÝĔĂĤĆòFRETĔĠģ

±F-ĕHĖºĂĤĚćħđăĊóĔòAkt1ĔàăĐ¢©xĕ±F-Ė¢©ÅĒÊăĐHü¸ġĤĚăĊóAkt1ĕ¢©xęĕI2ĖO-GlcNAcíĔĠĢTĂĤģāĒĖ



Ĕ8+ĂĤĐ÷Ěąó/ĕ¢©ĪŒŚķŘĲ]Ħ÷ĐòĈ

4. ¢©ĻŘŇıÅ Ýĕ'¹ ś"ÂiĠĢØf<ŜĤĒ¬ąģ¥uüSġĤòāĕ]ĕpYüĂĤĚăĊó
rÂiđ°¨ġĖýĊ¢©Ĕú÷Đ{ĻŘŇıÅĕ

Ý

Ħ'¹

ąģ]Ħ8+ăĚăĊó

{ĻŘŇıÅĔEăĐâüöģĞĕĕò>þĕ¢©ĻŘŇıÅĔEăĐāĕ]ĖÕ'ªđąóQāĕ]Ħ÷Đò¢©=ĔWąģ

Ý

Ħ'¹

qPĂĤĚąó

- 21 -

ăòÝĕĠĢ¾¢ē|ª»müć_FyęĥȢǿȨƲ Ʋ No. 51 (2016 July))0,)

 
 



hinaba@chem.tottori-u.ac.jp%&#ĝǚ20151ÐǘƍtyǙGƆęĥ~ǔIlȩyȪǬPƴ20162ÐǟǔǮțȠǳǙǰȠȋǰ

tyǮȨȍȈȧǻȝȥȔȨȥÚǔȗǽȇǵǕǈǓęĥǬœƺǟǈǎƵ20163ÐƾǦưPtyǙØð`>ę
ĥ~ǔC½ǬEǡǓƽǧǟǊƵcǚǮțȠǳǔǙĎyƬǬÎƾǌǓƺǎǏǁãǬƺǎǏƺǎǙǔƴ
ǍǙķƬǬƽƼǈǎƺǕ¦ƺǟǊƵ
#
cǙĎyǙ?ǘƴIlŨġfįÈǘǮțȠǳǙUCȍȨǵȢȨȩChristopher J. Changęĥ~ȪǘƕĘÓĎyǇǌǓƺǎǏǁãǬȦÐǟǈǎƵǍǙǕǀǘChrisǙęĥ~ǔȗǽȇǵǬǈǓƺǎǙƿƴĄfǰȠȋ

ǰtyǮȨȍȈȧǻȝȥȔȨȥÚFyĞǔęĥ~ǬáƼǓƺǨJefferson ChanǔǊƵĝǚǍǙĘÓĎyǙ
ƛǘJeffǕĺǘęĥǬǈǓƽǧƴeǢǥǁǣǧǕǧǈǓƺǟǈǎƵIlǬP

ǊǨē6ƿħǑǎǕǀǘǐ

ǤƻǖJeffƿāħǊǨǅǕǘǗǧƴȗǽȇǵǕǈǓÖǗƺƾǕmǬƾǃǓƺǎǏƺǎǙƿĎyǘŉǑǎĔ¹ǙąĊǔǊƵñqǘ7ǨǅǕǔÂǎǗ¿FǬyǜƴň8ǙęĥǬřǒǡĔǈǎƺǕłƼƴĎyǬë¨ǈǟǈǎƵǅƻǈǓłƼǨǕ»ǡǓĽǚt

ǏǕ¦ƺǟǊƵIlŨġÈǘfįǈǓƺǎƍtyþŶȫĴņ

ĹVǻǽȄȚ³ùȩiCeMSȪǚqeęĥŃƿrǁƴǟǎ\ǧǘĎyķƬŃƿrƺǅǕǢëÁǬ±ǈǈǟǈǎƵȗǽȇǵǕǈǓôİǊǨ?ǘJeffǙęĥ~ǬřyǊǨãƿƹǧƴțȥȍȨǙ?ǔIlŨġÈǙ
ęĥǬĐŖǈǟǈǎƵ
Ʋ)0,)(3-+2*/1.31

ǰȠȋǰtyǮȨȍȈȧǻȝȥȔȨȥÚȩUniversity of Illinois at Urbana–Champaign, UIUCȪǚǻǳǹƾǦ
ȍǽǔÈƕǞǖǙƴǮȨȍȈƴǻȝȥȔȨȥǕƺƻ

ǒǙŔǘǟǎƿǑǎtyǔǊƵoǚòǈǁ¥ÊǔƠǘƈǆǈǣǊƺĆjǔǊƵǎǏǈ5ǚÏ−20ƳƁǁǟǔƿǧƴƗǚ£ƥǔǊƵÏ:ǘėƺǎǕǀ
ǚƞƿǢǙǊǆǁƴǅǅǔǣǑǓƺǃǨǙƾǕ{ǘǗǑǎǅǕǬŜƼǓƺǟǊƵtyǬ¢ǕǈǎǇǗŔǗǙǔƍǙǥƻǗ#<ǇǚƹǧǟǌǭƿƴňûǘdǟǩƴŎǐėƺǓęĥǘƜǔǀǨĆjǔǊƵÏ:ǚŻƿ£ŘǏǕ¦ǑǓƺǟǈǎƿƴȍǽƿĐƉǈǓƽǧtyƖ$ŃǚúÀǔ!ĉǔǀǨǅǕƾǦƴĸŻǚ
ŷ/ǌǋǘȍǽǕňżŻǔňĊǗǁćîǔǀǟǈǎƵ
UIUCǙFyĞǚProf. LuǣProf. MooreǗǖŏXǗ-ćƿrǁƴyćǙȢȓȡǢƮƺǥƻǘ¦ƺǟǊƵ'- 22 -

ć_FyęĥȢǿȨƲ Ʋ No. 51 (2016 July)ĒǘǮțȠǳǔǚDăĒǘęĥǬœƺpÃǘǚzǊǨǕƺƻǰțȨǼǔǈǎƿƴÅÕǕnǫǦǋósǟ
ǔȌȨȇȤȨǵǬǈǓƺǨǙǬǥǁřƾǃǟǈǎƵty0ǕǈǓ{0īąƿ¡ǇǩǓƽǧƴ{0ȆȢȨȉȥǶǬQǃǗǃǩǛ}ƬƿǔǀǟǌǭƵ"ƼǛNMRõ|ǬœƻǎǡǘǚƴǍǙKąǣõ|ÃíǘƖǊǨ
ŢƬǬȎǽǊǨ£ŘƿƹǧǟǊƵty3ǘtǀǗŢŐ%īƿƹǧƴ£ŘǗÈǚǊǂǘǍǅƾǦŷ/ǔǀǨǎǡƴƠǘ#<ǔǊƵÃǔǮțȠǳǘƽǃǨŢŐīąǙŚ=ǙLǈǇƾǦƴ2Węĥ-ƾǦŢŐǬ
ƂǑǓƺǎǏǁǙǘ¾ȦÐƾƾǨǅǕǢƹǧǟǈǎƵFyĞǔǚçƅy3qƾǦÖǦǩǎ-ćǘǥǨ²ūøƿœǫǩƴÏÂǙęĥǬńǁǅǕƿǔǀǟǈǎƵXRtyǙ)Ƌ-ćǢ

ÅƕǘǫǎǑǓūø

ǇǩƴtǀǗOƣǬ^ǭǔƺǟǈǎƵĐŖǘǈƽţǈǔǀǎǙǢƺƺ¦ƺ7ǔǊƵUIUCƵňûůƾǗǴȝȥȎǽǔǊƵƲ Ʋ Ʋ Ʋ Ʋ ƲyiǘǓƴęĥ~țȥȍȨǕƵĕǭƿȖǽǙJeffƵ

# !
JeffǙęĥ~ƿĐŹǈǓÔöǕƺƻæƚǔMBǈǎǎǡƴęĥ~ǙħǐǄǘǢƖǫǧǟǈǎƵ£ŘǗãbǣŢŐǬȠǽȆǮȂȑǈÞŃǕ®ǐVǫǌǬǈǎǧƴȅȜĉǙŗĿǬ(ǧǓ}ƬıǬáĭǈǎǧǊǨǅ
ǕǚƴtnǔǈǎƿųƏǗķƬǕǗǧǟǈǎƵJeffǙęĥ~ǚĴņ3ǙŰþȩƒǰǲȥƴǻǶȈȡ8xƴƎĳî§ǗǖȪǕO¤ǈǓŒ.ǣ.ƢƣǻǶȈȡƿkǇǩǨ8xǾȥǺȨǙƔĐǬē¶ǈǓƺǟǊƵ
ĝƿMBǈǎÈǚV¬8xǘǥǨǾȥǺȨƔĐǙǠǬœǑǓƺǟǈǎƿƴĝǚIlŨġÈǙķƬǬîƾ
ǈƴŒ.őđŶǬƭÜǕǈǎęĥǬœƺǟǈǎƵŗĿǙ÷*ǣőđŶĐĄıǙáĭǬƾǦœƻǎǡrÈƕƿƾƾǧǟǈǎƿƴJeffƿÂǈƺ}ƬǬ&ƧǈǓǌǓǁǩǎǅǕƿwǈƾǑǎǕWÈǘƴǍǙŲƾǦźƿǀĻǟǨ¦ƺǔǈǎƵ
JeffǚŋǁǈǓāħǈǎǅǕƾǦƴƷUǕƌǕǈǓǔǚǗǁƴǟǋNǕǈǓŮŪǈǎƺƸǕŝǑǓƺǎǏ
ǀƴǍǙǽǿȥǽǬęĥ~ǙyćǘǢěǈǓƺǟǈǎƵǍǙǅǕǢƹǧƴęĥ~ǙțȥȍȨǚȖǽǣȗǽȇǵǏ
ƾǦǕƺǑǓnǘƊ«ǊǨǅǕǚǗǁƴđýǈǎȅǯǽǳȂǻȞȥǬƔǈǓƺǟǈǎƵň8Ǚ¨řǬŝƻǅǕ
ƿêǡǦǩǨǅǕǢrǁƴǍǙǥƻǗĆjǔºǟǩǨǅǕǚtǀǗķƬǘǗǧǟǈǎƵĎyǘtyƘćXƴyƌćXǬ¶ǊǨãǬƼǓƺǎǏǀǟǈǎƿƴuǦƿǎǏŝǫǩǎǅǕǬǅǗǊǙǔǚǗǁƴň

8ǔǣǨǝǀǅǕǬłƼǓƴ¨řǬǊǨvƿJŰǘåǑǓƺǟǊƵJeffǚéŽǘ}Ƭ~ǘŹǬƇǜƴ

}ƬǙƆœĀìǘƖǊǨĮHǗȅǯǽǳȂǻȞȥǬçÅǙǥƻǘœƺǟǈǎƵǮǰȅǯǮǬƦǆǗǈǘZ|- 23 -

ć_FyęĥȢǿȨƲ Ʋ No. 51 (2016 July)ǊǨǅǕǚǞǕǭǖǗǁƴǥǁţǈVǑǓƽƺĲǈǎǦǊǂǘ}ƬǘPǧ¸ƾǨǅǕƿǔǀǟǈǎƵ2WęĥǬĤßĒǘœƻǗǖƴǅǙȏȂȆȤȨǵǙŽǇƴëÁǙÆǇǚřŁǑǓƺǀǎƺùǔǊƵ
ęĥ~ǔǚçƅÐÌÅǘJeffǕǔȅǯǽǳȂǻȞȥǊǨindividual meetingƿƹǧƴ-ƅǙĸÙƴƅǙŞČǘǒƺǓţǈVƺǟǈǎƵǗǙǔŤĴǟǔŮŪǊǨǅǕƿǔǀǨƴÑ¨ŀǗÈƕǔǈǎƵǟ
ǎƴƅcǙęĥ~ǙșȨȄǯȥǶǔǚƴȩ

ǘJeffǙƽǆǧǔȪȅǯȈȨǬƫǝǗƿǦęĥh[Ǖ¿ĂĵƿœǫǩǟǈǎƵęĥh[ǔǚ}ƬĸÙǘǊǨȅǯǽǳȂǻȞȥǏǃǔǗǁƴǍǙŅÉǕǗǨęĥǣ}Ƭ
KąƴÖĒǗÒǗǖƾǗǧĴƾǁĦǑſǟǩǨǎǡƴǈǑƾǧ÷*ǊǨ£ŘƿƹǧǟǈǎƵ¿ĂĵǚçcǽǿǰȡƿďǗǧǟǈǎƿƴJŰǘåǑǓƺǨǙǚƴtǀǗęĥ8ƐȩĝǙiVGenetically-encoded
sensorsȪǬȄȨȘǕǈǓJeffƾǦƼǦǩƴǍǙäTƴŨƨƴÏÂǙęĥǬǟǕǡǓĐŖǊǨǢǙǔǊƵÿ|Ǚ8ƐǬƾǦũǝǨǙǚtnǔǚƹǧǟǈǎƿƴıĹĒǘyǜĔǊƺƺãǕǗǧǟǈǎƵǟǎƴțȥȍ
ȨƿȔǮǕǗǧƴÂǈƺęĥȄȨȘǬĐÝǈǓĐŖǊǨ“proposal competition”ǕƺƻŢǠǢƹǧǟǈǎƵ
ĝƿfįǈǓƺǨƕǘ

hǙŪ¿ƿęĥ~ƾǦĐŖǇǩǟǈǎƵǖǐǦǢĸÙǬ7ǈǓƾǦéǘÎǀǄƴ¯ģǊǨǽǿǰȡǔǊƵōŦȊǰȄǯȐǔƹǨǅǕǚǢǐǪǭǔǊƿƴ8xǾȥǺȨƔĐǕƺƻēĒƿ
ǚǑǀǧǈǓƺǨęĥ3ǗǅǕǢƹǧƴƹǦƾǉǡǰȥȆȣȀǵǻȞȥǬłƼǓƽǁƾǦǅǍǅǙǥƻǗƀƄǗ
¤ƿSŇǘǗǨǙǏǕ¦ƺǟǊƵ}ƬǬœƻ?ƾǦǰȥȆȣȀǵǻȞȥǬłƼǓƽǁǕƺƻǙǚgÕǔǚƹǧ
ǟǊƿƴǍǙƏŘ§Ǭ»ǡǓ©ǉǟǈǎƵ
"$



IlŨġÈǘfįǈǓƺǎiCeMSǔǚōŦƿ1ĉŦǕǈǓ!ǫǩǓƽǧƴĘÓĎyǢķƬǈǎǅǕƾǦƴ
ƹǨġōŦǚǔǀǨǕ¦ǑǓƺǟǈǎƵǈƾǈƴǣǚǧÅĒǘćîǊǨǔōŦAǚǟǏǟǏǏǗǕ©
ǉǨǅǕƿrǁƹǧǟǈǎƵÿǘȊǰȄǯȐǙōŦǚńǀPǧƿƝǈǁƴÞŃǕǙƟţǔǙǣǧPǧǚƾǗǧŌ
­ǈǟǈǎƵçÅęĥ~țȥȍȨǕǣǧPǧǬǊǨǅǕǔƶǘªǩǓǚǀǟǈǎƿƴōŦAYǙǎǡǘǚǕǘƾǁň8ƾǦĤßĒǘţǬǊǨǈƾǗƺǥƻǘ¦ƺǟǊƵJeffǚȟȖțȥȍȨǬƠǘt9ǘ¦ǑǓƽǧƴǮȎȨȆǘ/ǨǟǔǙȦÐǞǖňzǘǟǫǌǓƺǎ
ǏǁǗǖƴtnƽţǘǗǧǟǈǎƵǥǁJeffǙǔȕȨȚȎȨȄǯƿƔ+ǇǩǎǧƴŧƾǙťćÅǘǚǷȨ
ǴǬŴǑǓƽĜƺǊǨƴȖȨȠȥǶǣǹȡȏƴÇČǘœǁǗǖƴâƶǗęĥ~ǙǰȓȥȆƿƹǧǟǈǎƵǟǎ
ÅÕǕWǉǥƻǘęĥ~°ǙǽȗȨȃtƿƹǧǟǈǎƿƴơđƺǙƿƴLab OlympicǕğǈǓƴǺȂǳȨ
ǣȍȢȨȖȨȡƴȘǰȡŸƴȓȥȁȑȢǽƴĚ¯ǄǗǖrǁǙĢēǬĨƺVƻǢǙǔǊƵǅǩǦǬÅƾǃǓœƻǎǡƴĶǫǨǕȒȆȒȆǘǗǧǟǈǎƵ
ƕǔËǬPǩǨÅ¾ǚëǟǑǓƺǟǊƿƴǍǙĬd3ǔƹǩǛgÕĒǘƺǒǔǢËǬPǨǅǕ ƿ ǔ ǀ ǟ Ǌ Ƶ 8 Ð ǘ ǚ ƴ 2014  ǘ Q ŵ Ǉ ǌ Ǔ ƺ ǎ Ǐ ƺ ǎ SciFinder Future Leaders in Chemistryȩhttps://www.cas.org/products/scifinder/futureleaders-alumniȪǙQŵțȥȍȨƿǲȌǰǲǙǸȣȥȍǽǔ
4ǜƜǟǨãǬCASȩChemical Abstract ServiceȪƿšǃǓǁǏǇƺǟǈǎƵǅǩǚçčƾǦQŵǈ
ǎțȥȍȨƿƜǟǑǓSciFinderǣCASǘǒƺǓyǜƴňźǙęĥǘƖǊǨȅǯǽǳȂǻȞȥǬœƻȑȣǶȟ
ȚǔǊƵ?ǙQŵțȥȍȨǕ4ǈƴǟǎǙǙțȥȍȨǕïǬóǡǎĳÊǦǈƺķƬǕǗǧǟǈǎƵ
ǘ'ĒǗÄœǕǈǓƴǻǳǹǘǚęĥ~ǙĎyćǣÅÕƾǦÖǦǩǎNǕǥǁÅǧÄœǘœǀ
ǟǈǎƵǟǎƴǲȢǹȥȗȨȆȟȥȇǔǚĳÊǦǈƺƞȩMt. HoodȪǙƪÉǬĖǡǨǅǕƿǔǀǟǈǎƵǅǙ
ǥƻǘƴǮțȠǳe3ǬèžĒéŽǘÄœǔǀǨǙǢǮțȠǳĎyǙƯAǙǒǏǕ¦ƺǟǊƵęĥ~ǙyćÍǁƴƷtǀǗƍǚÅÕǘǢƹǨǙǏƾǦƴǮțȠǳǔǚtǗňûǬřǎÃƿƺƺƸǕǙǅǕǔǈǎƵ
- 24 -

ć_FyęĥȢǿȨƲ Ʋ No. 51 (2016 July)ǸȣȥȍǽǘǓƴ2014QŵțȥȍȨǕƵƲ Ʋ Ʋ Ʋ Ʋ Ʋ Ʋ Ʋ Ʋ Ʋ Ʋ Ʋ Ʋ “ǲȢǹȥl”ǕğǇǩǨMt. HoodƵ
'!
ƽǥǍǕƺƻĘƺÓƕǔǈǎƿƴÕĎyǚƾǃƿƼǙǗƺķƬǕǗǧǟǈǎƵǥǁŝǫǩǨǅǕǔǊƿƴ
Ąfǚ}Ƭš*ǣęĥĆjǚÅÕǙÃƿ,ǩǓƺǨǅǕǢrƺǕ¦ƺǟǊƵǈƾǈƴ¿FǢŝŦǢďǗǨ
ĆjǔƴƹǨ¨]ǥǍŃǕǈǓęĥćîǬƂǨǅǕǚƴ;Ǚśùǔþ

ǬřǨŊƺşļǕǗǧǟǈǎƵň8ǙǙǀ7ǈǕǈǓǘîƾǌǨǙǔǚǗƺƾǕ¦ƺǟǊƵǢǐǪǭĎyÓƕǘ¬ǈǎȊȂȆȤȨǵ
ǢtǀǗűĈǔǊƵ
ęĥ~ǔǙćîǬƃǉǓ

ǥǧJŰǘåǑǓƺǨǙǚƴțȥȍȨǙÕéƴýƑǔǊƵJeffƿŋƺǅǕǢ

ƹǨǕ¦ƺǟǊƿƴÃĒǘň8Ǚ¨řǬ±ǈǃǨǙǔǚǗǁƴǕǅǕǭȅǯǽǳȂǻȞȥǬǈǓțȥȍȨƿ
Ĳ

ǈǓƾǦ}ƬǘĠǨǅǕǬÒǭǔƺǟǈǎƵyćǢǍǩǘ¤Ƽƴň8ǙŝƺǎƺǅǕǬŝƺVƼǨĆj

ǔǈǎƵǅǙǥƻǘ½aǕțȥȍȨƿƹǨ¨]ĪǘţǈVƻǅǕǔǥǧ,ǩǎęĥǬē¶ǊǕƺƻǽǿǰȡǬ
yǜǟǈǎƵǢǐǪǭ¨řƿ8ƾǩǨǅǕǢƹǧǟǊƿƴƽƺǙ¨řǬÕéǔŝƺVƻ8ǫǏƾǟǧǚǗƺǥƻǘ¦ƺǟǊȩǎǟǘŕĦǈǓƺǟǈǎƿƴƴƴȪƵeįǣƱƴŅÉƿǛǦǛǦǗțȥȍȨǔǊƿƴǺ
ǰǱȥǽǬǣǨǎǡǘÖǓƺǨǕƺƻ2ƃƤǬµǒǅǕǔľƿǑǓƺǨǕƺƻ©ŜǔǊƵň8ǙęĥǙơđ
ǇǚǖǅǘƹǨǙƾƴÖĒǘǬē¶ǊǙƾƴǕƺǑǎǅǕǬÅĒǘƴƾǒĕ@ǘłƼǨǅǕǙt9Ǉ

Ǭ©ǉǟǈǎƵǟǎƴÅƶĩƩǔƈǆǈƴ·´ǬéµǐŊǁœƻǅǕƿÅƶƈǆǊǔ¨qǕtǢǮțȠǳǘÖǓyǭǏǅǕǙǒǔǊƵÛǘƹǨǙǚƴÅƶǙćîǬt

ǏǕƺƻǙǘƴàǈǁƈǆǊǅǕǬ¨ŭǈǓƺǨǕƺƻǅǕǏǕ¦ƺǟǊƵĄfǚ½aǕǈǓyćǬ¶ǊǨħiǕǗǧǟǈǎƿƴÕĎyǔ

ƺǕłƼǓƺǟǊƵÿǘƴň8ƿQǃǎ¶ǎrǁǙķƬǬyćǘƼǓƺǀǎ

ǙƾǦň8ǗǧǙÃƓǬ|ǡƴyćǘęĥǙàǈǇǬƼǦǩǩǛǕ¦ƺǟǊƵÏǘǗǧǟǈǎƿƴǅǙĎyƬŠǬÎǁãǬƼǓǁǏǇǑǎüÕtyǙK¼I-ćƴØðćǘ©ŬċǈǄǟǊƵǟǎƴęĥǚǢǕǥǧƴĎyǘƹǎǨ¢áƼǬǆ¶ƺǎǏƺǎG-ćƴ×ÞtyǙƐƙT-ćƴǍǈǓęĥ~ǙħǐǄǕƺƻƏŘǗÈÓǘĝǬQǃ/ǩǓǁǏǇǑǎJeffǘ¢ǥǧ©ŬċǈǄǎƺǕ¦ƺǟǊƵ- 25 -

["-hkÚÂÞ  No. 51 (2016 July)

第 19 回生命化学研究会〜生命化学・温故知新〜

https://res.tagen.tohoku.ac.jp/FBC/

2016

8

1

13:30 ~ 2

12:00

(https://www.kgh.ne.jp/10/access/)
60
90

1. JR
2.

)
JAL 2910800-

ANA 3811

0720-

0935,
0900

3

0845-

1038

545-

731(

)

541

0943-

1020

8,000

0804-

0943-

18,360E-mailtsumu @ b.s.osakafu-u.ac.jp @

)

1043

6

20

)
40
6

20
A4

b.s.osakafu-u.ac.jp

tsumu @))
(https://res.tagen.tohoku.ac.jp/FBC/))@(

599-8531
TEL

1-1

072-254-9834- 26 -_____

["-hkÚÂÞ  No. 51 (2016 July)

第 10 回 バイオ関連化学シンポジウム（第 31 回 生体機能関連化学シンポジウム、第 19 回 バイオテクノロジー部会シンポジウム）

http://jointsympo.csj.jp/

KN-

-[

Sv-

KN-ß[-ÝË¶¹Å»ÊÛÀÞÆµÍÀÖÜàKNz-

2-ÎÛÜÅµ´["-hkË¶¹Å»ÊÛÀÞÒÁÇ-½ÁÇÝ,-hk

9 M 7 KßTàâ9 Kßà
JR
6 29
7 15
7

22
)

1
4

2
5

6

◎
◎
*

7

1

15
WEB

7

3

2
5
1
1
http://jointsympo.csj.jp/
7,000
9,000
9,000
11,000

22
9

8

2

3,000
4,000
5,000
6,000

ANA
8,000
WEB

/
923-1292

http://jointsympo.csj.jp/

1-1

10/FAX (0761)51-1650
E-mail: takagi@jaist.ac.jp
http://jointsympo.csj.jp/- 27 -

KN[Y["-hkÚÂÞ  No. 51 (2016 July)

第 43 回国際核酸化学シンポジウム
The 43rd International Symposium on Nucleic Acid Chemistry 2016, ISNAC2016
http://isnac2016.org/

R-¿ÜÓÀ·Õos+$
KN-

KNz-

2016

27

9

KNQ-,-,>`XN(-

29
5860-0047

1. Chemistry of Nucleosides, Nucleotides, and Their Analogues
2. Medicinal Chemistry of Nucleosides and Oligonucleotides
3. DNA/RNA Chemistry and Biochemistry
4. DNA/RNA Structure and Recognition
5. Ribozymes, siRNAs, and miRNAs
6. DNA/RNA Materials and Diagnostics
7. Drug Delivery Systems and Nanotechnology of Oligonucleotides
 d}_á2016 4 4 M 11 K6 M 17 K
 LEá2016 4 4 M 11 K7 M 8 K eá2016 4 4 M 11 K8 M 12 Ke yá 30,000 6K 35,000        -[á 10,000 6K 15,000 d}_¡°LEeª©¬¢®
¦¬?q­·¸Ï¾¶Çßhttp://isnac2016.org/à³p^¡¦{¤¦ e¬F@
­»ÚÀÄÇºÞÈ¯£­{C§¡¯¢
E-mail: isnac2016@nta.co.jp
Tel: 096-342-3873, Fax: 096-342-3679- 28 -

1-14-1

["-hkÚÂÞ  No. 51 (2016 July)

第 26 回 バイオ・高分子シンポジウム
http://www.spsj.or.jp/entry/
7

28

(

)

29

(

)

28

(

)

9
)
)
(1)
(2)
(3)
(4)
(5)
(6)

1.
2.
3.

20

12

8

4
1)



2)

7,560
5,400
3,240
5,000

2,500

http://www.spsj.or.jp/entry/- 29 -

[#.hkÚÂÞ  No. 51 (2016 July)  ß2). IEà

4>2756 m33& LO.

.|ÑÐÃÈ¼×ÎÇ-«±²b<ovZaf

-9-¿ÁÅÕ

ß201653Nà ß). IEà
4>2756 m33& LO.
[.|-«'¥£Tw;Ë¶¹ÔÅØ´Ù¬>
ß201653Nà ß"0). IEà

4>2856 j.B|¬Kj.)x}8 j.B| hkÉÊË¶¹ÆË¶Á~¨½ÊÕc:\«¢²hk
ß201654Nà
 

ßP

). IEà4>2856 j.B|¬Kj.)x}8 j.B| hk[=(-³\£n/1«¢²hk
ß201654Nà

 

ß

). IEà

m21& LO+;j.u¬
ÂÜÌ»

g

*

\³J¢²!>-¬~ß201655Nà ċ¿Ä½ģŽĽÄĹĿò úö
2016Û4Ć1ýz
E-mail: shin.mizukami@m.tohoku.ac.jp- 30 -

[#.hkÚÂÞ  No. 51 (2016 July) 4Ć14ý¾5/16ýĉĂ,Ʋà,¿*²Ɯ'ĢĊ-$&*ý,Ŵ*đ,o½,ü6Ơ

ą

ŬŞ

0),ũÌ;¢1,ŮŢç+ę21

,Ŭ*

,·;

7

&ç6Ļ;Ĭk1¿Ä- 5Ć9ý6ÄĨñ#&ŶŔ-17áĹĿË46Ćoÿ6
6Ï%$ÊƩ;Ɛ8('&
¶ª9&

1Ü

Ģ¿'4²Ɯ+58ÄĨ,ëð-0(<)

1<ē¯Ƥ*),żĩ+ƒ&-ĹĿË,ũÌ-ħ¿'96ļ",Ċá,æþÁ18,491< y°4:&l#,»§ƭNo. 51Ʈ;Ñ8('1èoŎ37;Æ#&³Ņĳ<+-ç6ĻĬk1Ĕ§ƭNo. 52Ʈ-ČĜ<,õá+572016Û10Ćơ,įŦ;rÉ&71TaiJeMiø®,1lűŏƚõá1'ƃō;!9.Ü

3ĳ<6,ūćìŬņ;ã"&7'

4>285 6N23L HXO).). lj.hkßtoshi@chem.kumamoto-u.ac.jpàsC7 )i] W-ß ).à
 QV $ß).à- 31 -