レターNo. 13 (2004 年 1 月)

１．

巻頭言
2

新しいステップに．．．
和田健彦 （大阪大学大学院工学研究科）

２．

第 1 回生命化学国際シンポジウム (ISBC 2003) 報告書
ISBC2003 組織委員会 委員長

３．

馬場嘉信

グラビアページ
写真で見る第 1 回生命化学国際シンポジウム

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4

6

Proceedings Book から抜粋
Preface, Session Overview

10

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プログラム

17

６．

プレ・ポストコン報告

34

７．

お知らせコーナー

42

受賞・会員異動のお知らせ、編集後記

生命化学研究レター No. 13 (2004. January) 2

新しいステップに．．．
大阪大学大学院工学研究科
和田健彦
2004 年が始まりました。2003 年は皆様ご存じのように生命化学、そして生命化学研究会にとって記念すべき年となりました。まず生命化学にとってはヒトゲノム・シークエンスプロジェクトが完了し、ヒト遺伝子の信頼性の高い全配列が決定されました。これから本格的に始まる遺伝子配列解析により膨大な情報・機能が明らかにされていくことが期待されますが、既に数多くの疾患・疾病遺伝子、ガン遺伝子や機能性遺伝子が明らかとされ、遺伝子工学・分子生物学の飛躍的な発展に貢献するのみならず、遺伝子診断、オーダーメイド医薬品の開発などへの展開も精力的に検討されています。当初 DNA→RNA→タンパク質へと一方向な生命情報の流れ、セントラルドグマに基づきヒト DNA の全遺伝子配列を決定することができれば、人間の生命情報設計図が明らかとなり、DNA エクソンと各タンパク質一次構造の一対一対応はもちろんのこと、その発現制御機構やリン酸化、糖鎖合成などの情報制御プログラムまでが明らかになるとの期待が一般の方のみならず、一部の研究者からも寄せられていました。しかし、10 万種類程度と推測されているタン
パク質数に対して、明らかとされたエクソンの概数は 34000 程度と約 1/3 しかなく、エクソンシャッフリング・
組み替えによるアミノ酸配列のコーディングや、一つのエクソンが複数種類のタンパク質一次情報をコードしている可能性が確認され、当然ですが生命情報は遺伝子配列だけで全てが決定されるのではなく、酵
素・タンパク質、糖質などとの相互作用や環境などの後天的因子によっても大きく左右されることが明らかとなりました。つまりヒトゲノム・シークエンシングプロジェクトの完了は、バイオインフォマティックス（生命情報科学・生体機能情報学・生物資源情報基盤）への新しいステップと考えられます。
一方、生命化学研究会にとっても 2003 年は記念すべき年となりました。生命化学研究会第 2 期目の大きな目標として会員の皆様とともに企画・運営活動を進めて参りました国際シンポジウム、International
Symposium on Biomolecular Chemistry 2003 (ISBC2003)を、馬場会長を運営委員長として兵庫県立夢舞台国際会議場で開催することができ、23 件の招待講演と 124 件のポスター発表が行われ、世界 15 カ国から 210 名もの方に御参加いただきました。I. Functional DNA/RNA，II. Cell Function of Macromolecules，III.
Metals in Chemical Biology，IV. Protein-Protein Interaction，V. Technology Innovation in Biomolecular Chemistry の
5 セッションの招待講演では、文字通り生命化学の世界トップレベルの研究成果を拝聴することができ、自然と質疑応答にも熱がこもり、大変盛り上がりました。招待講演・質疑応答により、多くの参加者は知的好奇心を大いに満足させるとともに、化学者の視点から生命現象解明・生命機能制御に取り組むことの素晴らしさに感動し、モティベーションを奮い立たせる素晴らしい時間を過ごすことができたと思います。ポスターも、第一回生命化学研究会国際シンポジウムに相応しい国際色にも富む内容の濃い発表が多く、組織委員・プログラム委員が 6 名のポスター賞該当者を選択するのに大いに苦労しました。また、この国際シンポジウ

生命化学研究レター No. 13 (2004. January) 3

ムの内容は、丸善出版から Biomolecular Chemistry – A Bridge for the Future – と題する 400 ページを超える本として出版されました。このように組織委員・プログラム委員の皆様のご尽力と会員の皆様のご協力により、生命化学研究会として期待以上の国際シンポジウム開催を実現することができ、組織委員の一人としてこの場をお借りして心から御礼申し上げます。
このように生命化学研究会は杉本直己初代会長、現馬場嘉信会長のご尽力・リーダーシップならびに役員・会員の皆様のご協力により第一期の目標である成書の発行、第二期の目標である国際シンポジウムの開催とも予想以上の成果を上げ、順調に発展していると思います。しかし、第二期目を終えようとした 2004
年、21 世紀における生命化学研究会の発展的飛躍を実現するためには・・・このままでいいのでしょうか？
1998 年 3 月「生命および生体分子の関与する化学」を基礎から応用まで広く研究・展開し、関連学問ならびに利用技術の一層の発展を図ることを目的に設立された時、生命化学研究会は 1.平均年齢が若い 2.
ヘテロな集団である この二つの特長を有していると杉本会長が指摘されています。若いと言うことは、既成概念にとらわれず、斬新な企画・アイデアに富んでいることを、ヘテロな集団であることにより画一的でな
く色々な視点からの意見・アイデアが期待出来ることを意味されていると思います。実際に第一期・第二期において従来の研究会には見られない、斬新な企画・運営が実現されてきたと思います。しかし 3 年の研究会準備段階から数えると足かけ 10 年の月日が過ぎようとしています。ヘテロな集団であった研究会メン
バーも顔なじみとなり、いい意味でも悪い意味でもホモジニアスな集団になってきている気がしないでもありません。また物理的に年齢も重ねてきています、もちろん精神年齢は皆さんまだまだ若く、学生より元気な方もいらっしゃいますが…。ISBC2003 国際シンポジウムを盛会に終えた今こそ、もう一度設立時の原点に戻り若干形式化しつつ感のある企画、シンポジウム、ニュースレター、研究会運営を見直し、色々な考え方を受け入れる「脇は甘く、懐の広い」研究会に立ち戻る必要が有るようにも感じます。第三期の浜地 格新会長の下、意見・方向性を一つにまとめる必要はなく、失敗してもいいから色々な企画やアイデアを試してもいいのではないでしょうか？その中から、新しい方向性・意見が出てくる、でもその方向性などにも固執することなく、軽やかにフレキシブルに変化していく…それがヘテロな集団の持つ強みで有り、飛躍的な発展を遂げる重要なファクターでは…と思います。私は以前から一見関連はないと思われる異分野を融合した
時こそ、大きな発展が期待されることを信じて研究を行ってきました。ポスト・ゲノムシークエンスの今こそ、真の意味でのヘテロな集団に立ち戻り、純粋な分子生物学・生物学を専門とする研究者にも参加頂き、化学者の得意とする分子レベルでの生命現象の理解という視点と融合することにより、Specialize された個々の生命現象の解明とその一般化・支配原理の解明いわゆる Generalize のバランスがとれた「生命および生体分子の関与する化学」研究の飛躍的な発展を実現しましょう。また、これからどんどん知的好奇心と向上心に富んだ若い研究者にも研究会に参加頂き、研究者の年齢的にもヘテロな集団でありたいですね。この研究会の本質は、研究成果を求めることだけでなく、それ以上に研究者が自分の知的好奇心を満足させ、
自分の向上心を煽ることにあると思いますから・・・
2004 年、生命化学研究会も新たなステップを・・・（わだ たけひこ：hiko@chem.eng.osaka-u.ac.jp）

生命化学研究レター No. 13 (2004. January) 4

第 1 回生命化学国際シンポジウム (ISBC 2003) 報告書
First International Symposium on Biomolecular Chemistry
ISBC2003 組織委員会 委員長

馬場嘉信

2003 年 12 月 2 日（火）〜5 日（金）、兵庫県立淡路夢舞台国際会議場において、上記国際会議を、ISBC
2003 組織委員会（委員長 馬場嘉信）の主催で開催した。この会議は、生命化学関連の基礎的学術研究、
生命分子を利用した薬物設計、さらには新規かつ広義の機能材料学の基礎と応用に関する会議であり、世界で初めて、日本化学会生命化学研究会の主催で、わが国で開催したものである。
日本化学会生命化学研究会は、生命現象を化学あるいは分子の観点からとらえ、生命現象の本質を化学的に理解すること、ならびに生体機能に学びあるいはそれを応用し、生体分子由来の新しい機能分子の創製を行うことを目的としている。特にヒトゲノム解析の進展を踏まえ、単に生命を真似るのではなく、思うがままの構造・機能を有する分子・分子システムを創製・構築する“テーラーメイド・バイオケミストリー”の実現を提唱している。さらに、核酸、タンパク質、糖、細胞などの各分野で活躍する様々なバックグランドを有する生命化学研究者が情報交換・議論を行い、分子レベルでの生体分子間での相互作用を明らかにする
ことにより生命化学の「ニューセントラルドグマ」を構築できるのではないかと考えている。これらの成果を応用することにより、SNPs をはじめとする遺伝子診断・検出法の創製、さらにはゲノム創薬のみならずプロテ
オーム、グライコーム情報に基づいた創薬をはじめとする従来法とは全く異なるような方法論を提唱できると考えている。我が国における生命化学研究者は多数におよび、特に近年我が国の研究者の活躍はめざ
ましいものがあり、生命化学に関する基礎および応用研究に関する世界第一線の研究者が一堂に会し、更なる最新の研究成果および今後の展開について発表、意見交換を行い、当該分野における国際的交流と発展を促進することを目的として、第１回生命化学国際シンポジウム（ISBC2003）を淡路島において開催したものである。
本会議は、DNA、たんぱく質、ペプチド、糖鎖、生体関連高分子、細胞などの生命化学的研究ならびにその応用に関する研究発表と意見交換の場を提供するために、本分野研究の最先端の研究者 25 名で構成されるプログラム委員会において、(1) 機能性 DNA・RNA、(2) 生体・合成高分子の細胞機能、(3) 金属イオンの化学生物学、(4) タンパク質―タンパク質相互作用、および(5) 生命化学における技術革新の５領域を中心的課題として取り上げ、基礎から応用に至る広い範囲を研究発表および討論の対象とした。
まず、それぞれの領域で、世界的に活躍している比較的若手の研究者 23 名を招待講演者として選んだ。

生命化学研究レター No. 13 (2004. January) 5

また、124 件のアブストラクトが投稿され、6 月末にプログラム委員会において、各アブストラクトの審査と分野の選定を行った。このように今回の学会は、世界中から選りすぐられた重要な研究発表が多数発表され、生命化学における世界の最先端の研究の状況を把握する上で欠かすことのできない学会となった。さらに、学会参加者も 200 名以上となった。
口頭発表では、非常に幅広い研究分野における世界最先端の研究成果が発表され活発な討論が展開された。また、ポスター会場も2時間のポスター発表時間中ずっと会場が満員の状態で大変熱気に満ち溢れた学会となった。発表内容は、(1) 機能性DNA・RNAが招待講演4件、ポスター発表29件、(2) 生体・合成高分子の細胞機能が、招待講演4件、ポスター発表17件、(3) 金属イオンの化学生物学が、招待講演5
件、ポスター発表19件、(4) タンパク質―タンパク質相互作用が、招待講演4件、ポスター発表35件、および(5) 生命化学における技術革新が、招待講演6件、ポスター発表24件、と各分野バランスよく発表がなされた。
この会議は、まさに生命化学の基礎と応用を中心課題に据えた世界で唯一の国際会議であった。DNA、
たんぱく質、糖鎖、細胞にいたる幅広い範囲において、この会議は、その学術的意義とともに、将来のゲノム情報に基づく総合的健康管理を実現するという社会的な意義を併せ持つ点に特色があった。生命化学
国際会議の初めての日本開催は、最近のわが国の研究者の研究成果に対する国際的評価の反映であり、今回とくにわが国の貢献が顕著である分野の進歩を中心的な課題として取り上げることは、これらの分野の一層の発展をもたらすものと期待される。また、生命化学に関する国際会議を、バイオ分野研究進展の著
しいアジアで開催できたことは、国際的にも意義深いものと考えられる。
本会議の内容は、研究発表と討論の成果を世界に公表するために丸善からプロシーディングスとして発刊した[1]。各発表者が、各研究内容を 2-6 ページにまとめたもので、400 ページにもおよぶ本となっている。
本会議終了後には、世界中の研究者から、本会議が、史上最も価値のある会議であったとの賞賛を多数いただいている。

[1] Scientific Program Committee of ISBC 2003 (Ed.), Biomolecular Chemistry, Maruzen, 2003, pp. 1-387.

BIOMOLECULAR CHEMISTRY– A Bridge for the Future –Awaji Yumebutai International Conference Center, Hyogo, Japan

December 2-5, 2003
Organized by
the Forum on Biomolecular Chemistry,
the Chemical Society of Japan

PREFACE
The First International Symposium on Biomolecular Chemistry (ISBC2003) will be
dedicated to the latest scientific and technological developments in the diverse fields
of modern chemical research into DNA/RNA, proteins, carbohydrates and metals,
with particular emphasis on understanding and controlling the phenomena in living
systems.
ISBC 2003 is organized by the Forum on Biomolecular Chemistry, the Chemical
Society of Japan. In 1996, the discussion group on Biomolecular Chemistry wasformed by young, active and motivated scientists with a chemistry-based approach tobiology, inspiring a number of meetings to discuss the future of Biomolecular
Chemistry: Kumamoto (1996), Hakone (1997), and Tokushima (1998). Arising fromthese discussion meetings, in 1998 the group proposed and organized the Forum on
Biomolecular Chemistry within the Chemical Society of Japan, one of the largest
scientific societies in the world. The first symposium on Biomolecular Chemistry washeld in Okazaki in 1999, bringing together scientists interested in biology-oriented
chemical studies on DNA/RNA, proteins, carbohydrates, metals and cells; this was
succeeded by the second symposium held in Osaka in 2000, and the third symposium
held in Kobe in 2001. During these meetings, the members of the Forum discussed
the future perspectives of this field in the post-genome sequencing era and the twenty
first century. Arising from the realization of the multi-disciplinary and multi-national
nature of this scientific area it was decided to organize the first International
Symposium on Biomolecular Chemistry in 2003, whilst continuing to discuss what
the future concepts of Biomolecular Chemistry at the fourth symposium held in
Yokohama in 2001 and the fifth symposium held in Uji in 2003. Indeed, although
ISBC 2003 is the first international symposium, this meeting is the sixth symposium
in an already well-established series for the Forum on Biomolecular Chemistry within
Japan.
We have over a hundred submissions of contributed papers, and the programcommittee has worked very hard to arrange an attractive program. In addition, we
have excellent invited speakers from Europe, Asia and North America. We would liketo express our gratitude to the organizing and program committee members for theirdiligent work, and the excellent arrangements could not have been made without theefforts of Proactive Convention acting as the secretariat of ISBC 2003. Finally wewish to thank the most important people of all, the invited speakers, the technicalcontributors and participants! We hope all participants will enjoy ISBC 2003.
Yoshinobu Baba ISBC 2003 Chairman
September 1, 2003

–I–

Chapter 1. Functional DNA/RNA

Overview
I. Functional DNA and RNA
The completion of the human genome sequencing project has driven our researchinterest and endeavor to the creation of not only pharmacogenomic/pharmacogenetic
technology and gene therapeutic drugs using antisense and antigene strategies, butalso functional DNA/RNA, such as DNA/RNA aptomers, Ribozyme, DNAzyme,
siRNA and so on. Although we have acquired a degree of information about the
primary sequence of essential exsons and promoter regions of DNA, we are still justat the beginning in terms of understanding the relationship between the structureand function of DNA/RNA; for example, the driving force and rational regulations
for RNA folding and tertiary-structure formation of DNA/RNA. In addition topharmacogenomic and pharmacogenetic areas, technology is developing at a very
fast pace, but is not yet feasible in large applications. The chemical synthesis andchemical modification of DNA/RNA is the fundamental technology that has led themolecular biology revolution. Hence, the chemistry of DNA, RNA and nucleic acids
not only in vitro but also in vivo is expected to open a new generational stage ofbioorganic chemistry and molecular biology. If we can more fully comprehend therelationship between the structure and functions of DNA and/or RNA, we should be
able to design powerful tailor made functional DNA/RNA.
Therefore, the completion of human genome sequencing project is just startingpoint of the Bioorganic Chemistry of nucleic acids.
(Takehiko Wada)–3–

Chapter 2. Cell Function of Macromolecules

Overview
II. Cell Function of Macromolecules
In the research field of Biological Chemistry in post-genome project era, not only
the studies to know the role of biomolecules in cells but also the techniques to utilize
their function are needed. To make clear the function of macromolecules such as
nucleic acids, proteins and carbohydrates in cells, it is necessary to develop the new
analytical method. Recently, monitoring the dynamic phenomena of molecules inliving cells was achieved by the improvement of fluorescent probes and fluorescencemicroscopy to investigate in their subcellular localization and their intermolecularinteractions. The technology to specify the structures of bio-macromolecules has
been also promptly improved in these days. Furthermore, high-throughput analysesof the sequence of nucleic acids and proteins were achieved by the development of
sequencer. On the contrary, the sequence analysis of oligosaccharides as one of themetabolic products has not been established so far. However, the improvement ofmass spectrometry will make it possible to analyze the structure of oligosaccharide
conveniently and rapidly as well as peptides. In future, glycomics for the analysisof the expressed structures and functions will be improved as the same speed withgenomics and proteomics.
Although the research of high-throughput would be available for the first screening,
we should not make light of the experiments of low-throughput for the researchusing living cells. It is expected that investigating the function of macromoleculesin cells will be an outgrowth of the extended researches. One of them would be thedevelopment of medicine. The identification of the molecules related with a diseaseand cell surface receptor will lead to molecular-target treatment involving protection
from infection and tumor metastasis. Furthermore, for drug delivery system (DDS),
although passive targeting by a physico-chemical features of DDS was the maincurrent, active targeting by a recognition device to deliver the medicine involving
nucleic acids and proteins into target cells will show the progress more and more in
future. Especially for the development of gene delivery system using non-viral vector,
understanding the intracellular trafficking is very important to enhance the transfection
efficiency of plasmid DNA or siRNA. Peptides and oligosaccharides would be usefulbiomolecules to control subcellular localization of nucleic acids.
Understanding and controlling the cell function will lead to the exploration andelucidation of living system of the human body (in vivo chemistry). The research fieldon cell function of macromolecules is just a prologue for it.
(Toshinori Sato)– 87 –

Chapter 3. Metals in Chemical Biology

Overview
III. Metals in Chemical Biology
Over a few decades, the relationship between the role and nature of eachbiologically essential metal has been well defined by structural and mechanistic
studies of macromolecules as well as by synthetic inorganic chemistry. However,despite recent rapid development in spectroscopic and crystallographic techniques,
it is easy even for modern science neither to describe nor to recreate the metalindependent chemical behaviors accurately at the molecular level. In this session,
extensive structural and mechanistic works on the natural systems themselves aswell as excellent synthetic models, that best mimic and elucidate the stoichiometricand functional properties of the metalloenzyme active sites including multi-metallic
centers, will be presented. As more details about biologically essential metals andmore excellent synthetic bioinorganic molecules become available, more chemists,biologists, and physicists will become interested in this diverse and fascinatingchemical area. We hope that many participants will be aware of new projects that havebeen recently launched, and that this session will be catalytic for all the participants,
whetting their appetites for new directions in the widespread bioinorganic chemistry.
(Mitsuhiko Shionoya)– 141 –

Chapter 4. Protein-Protein Interaction

Overview
IV. Protein-Protein Interaction
With the human genome sequenced, attention is turning to the final product ofthat genome: proteins. The large number of cellular processes involve specifi c
protein-protein or protein-nucleic acid interactions, including signal transduction,transcription, cellular trafficking, and mitosis. Understanding these interactions has
mostly depended on the tools of genetics so far. In genetic approaches, a gene isdeleted or altered and then the effect on the interactions is observed. However, there
can be problems with traditional genetics. For example, most genetic mutations are
not conditional; they can not be turned on or off at will. Now, geneticists are realizingthat chemists can make an important contribution by designing specific moleculesthat can selectively disable particular protein-protein or interactions. Chemists aredeveloping a rich variety of chemical approaches that enable us to understand andmanipulate protein-protein interactions, chemical genetics, combinatorial chemistry,
directed evolution, and so on. The analysis of such protein-protein interactions,
whether qualitative or quantitative, presents a tremendous challenge for chemists. Thishot topic is the subject of this session.
(Ikuo Fujii)– 213 –

Chapter 5. Technology Innovation in Biomolecular Chemistry

Overview
V. Technology Innovation in Biomolecular Chemistry
Completion of the deciphering of the human gnome was declared on April 14,
2003, after 10 years of endeavor by an international collaboration of scientists. Thisfeat relied heavily on innovations made in the biotechnology area, particularly DNA
sequencing. Biomolecular chemistry, which deals with the clarification of various
phenomena associated with life at the molecular level, has contributed tremendously
to this innovation by providing numerous novel technologies that have helped
analyze the phenomena of life. In relation to this, biomolecular chemistry has also
generated new artificial systems bearing un-natural functions under certain conditions.
Furthermore, it is strongly anticipated that technologies derived from biomolcularstudies will provide powerful new analytical tools in biological, medical and relatedareas. They include DNA and RNA aptomers, combinatorial chemistry and the specificincorporation of artificial amino acids into proteins, along with new technologies
connected with microfluidics, DNA- and protein-chips. These technologies developed
thus far not only provided simple tools for research in this area, but have also
produced materials of commercial value which contribute to human welfare.
In the 21st century, the areas connected with biomolecular chemistry willcontinue contributing to industry more than ever. These technologies have beendeveloped mostly at universities and companies, however, it seems to be importantthat the connection between universities and companies is strong enough withoutcompromising scientific independence for this new area of science.
Herein, papers connected with the new sciences and technologies of biomolecularchemistry are collected. They include research not only to meet the current social
demands but also to create new science fields and business markets.
(Shigeori Takenaka)– 301 –

生命化学研究レター No. 13 (2004. January) 17

PROGRAMINVITED LECTURES
Wednesday, December 3 (Main Hall )Session I. Functional DNA/RNA
9:00-12:00 (Chairpersons: Peter E. Nielsen and Naoki Sugimoto)
Proceedings Abstract
OI-1
Regulation of Gene Expression by Peptide Nucleic Acid Antisense. Targeting424
the mdm2 Oncogene
Takehiko Shiraishi, and Peter E. Nielsen
Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen,Blegdamsvej 3, DK2200, Denmark
OI-2

Ribozymes for New Genetic Coding Systems
824
Hiroaki Suga†‡, Hiroshi Murakami†, and Dimitrios Kourouklis‡†
Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku,
Tokyo 153-8904, Japan, ‡Department of Chemistry, University at Buffalo, The State University of New York,
Buffalo, NY 14260-3000, USA
Coffee Break (10:20-10:40)

OI-3

Modified Nucleic Acid Systems: Design, Synthesis and Evaluation
12
24
Byeang Hyean Kim
National Research Laboratory, Department of Chemistry, Division of Molecular and Life Sciences, Pohang
University of Science and Technology, Pohang 790-784, Korea

OI-4

How Proteins are Transported into and out of the Nucleus
16
24
Yoichi Miyamoto, and Yoshihiro Yoneda
Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, 2-2
Yamada-oka, Suita, Osaka 565-0871, Japan

Session II. Cell Function of Macromolecules
15:00-18:00 (Chairpersons: Kay-Hooi Khoo and Koichi Fukase)
Proceedings Abstract
OII-1 Carbohydrate Recognition Systems in Cell-Cell Interactions and Signaling
88
24
Reiji Kannagi
Department of Molecular Pathology, Aichi Cancer Center, Nagoya 464-8681, Japan
OII-2

Automated Solid-Phase Oligosaccharide Synthesis
92
24Peter H. Seeberger
Laboratory of Organic Chemistry, ETH-Hönggerberg, Zürich,8093, Switzerland and The Burnham Institute,
La Jolla, CA 92037, USA
Coffee Break (16:20-16:40)

OII-3

OII-4

(Chairpersons: Peter H. Seeberger and Toshinori Sato)
Sialic Acid Synthase from Escherichia coli- Structural Characterization by
96
24
Mass SpectrometryYu-Ju Chen†, Tzann-Shun Hwang‡§, Xing-Hung Hwang†, Hsin-Kai Liao†, and Chun-Hung Lin‡§†
Institutes of Chemistry, Academia Sinica, Taipei, Taiwan, ‡Institute of Biochemical Sciences, National Taiwan
University, Taipei, Taiwan, §Institutes of Biological Chemistry, Academia Sinica, No.128 Academia Road
Section 2, Nan-Kang, Taipei, 11529, Taiwan
Mass Spectrometry Profiling and Sequencing of Complex Glycans in
98
24
Glycomics and Glycoproteomics
Kay-Hooi Khoo
Institute of Biological Chemistry and Core Facilities for Proteomics Research, Academia Sinica, Nankang,
Taipei 11529, Taiwan

17

生命化学研究レター No. 13 (2004. January) 18

Thursday, December 4
Session III. Metals in Chemical Biology
9:00-12:00 (Chairpersons: David P. Giedroc and Mitsuhiko Shionoya)
Proceedings Abstract
OIII-1 Structure and Function of the Heme-Based Sensor Proteins142
24
Shigetoshi Aono
Center for Integrative Bioscience, Okazaki National Research Institutes, 38 Nishigo-naka, Myodaiji, Okazaki,
Aichi 444-8585, Japan
OIII-2 Helix-Inducing Metal Clips in Short Peptides
146
24
Renee L. Beyer†, Michael J. Kelso†, Huy Hoang†‡, Trevor Appleton‡, and David P. Fairlie††
Centre for Drug Design and Development, Institute for Molecular Bioscience, ‡Department of Chemistry,
University of Queensland, Brisbane, Qld 4072, Australia
OIII-3 Oxygenation Mechanism of Phenols by Dinuclear Copper Monooxygenase150
24
Shinobu Itoh†, Shin-ichi Yamazaki†, Hideyuki Kumei‡, Masayasu Taki†, Takao Osako†, and Shunichi
Fukuzumi‡†
Department of Chemistry, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto,Sumiyoshi-ku, Osaka 558-8585, Japan, ‡Department of Material and Life Science, Graduate School ofEngineering, Osaka University, CREST, Japan Science and Technology Corporation, 2-1 Yamada-oka, Suita,
Osaka 565-0871, Japan
Coffee Break (10:30-10:50)(Chairpersons: David P. Fairlie and Kazuya Kikuchi)
OIII-4 Molecular Interaction and Protein Folding of Plastocyanin
156
24
Shun Hirota
Department of Physical Chemistry, Kyoto Pharmaceutical University, Misasagi, Yamashina, Kyoto 607-8414,
Japan
OIII-5 Coordination Chemistry and Allosteric Switching in Bacterial Metal Sensor
160
24
Proteins
Mario A. Pennella†, Christoph Eicken‡, Laura S. Busenlehner†, Xiaohua Chen†, Michael L. VanZile†, James C.
Sacchettini‡, and David P. Giedroc††
Department of Biochemistry and Biophysics, Centers for Advanced Biomolecular Research, ‡Structural
Biology, 2128 TAMU, Texas A&M University, College Station, TX 77843-2128 USA

Session IV. Protein-Protein Interaction
15:00-18:00 (Chairperson: Don Hilvert)
Proceedings Abstract
OIV-1 Developing Chemical Biology Tools for the Study of Functional Proteomics
214
24
Lai-Peng Tan†, Resmi C. Panicker‡, Lay-Pheng Tan†, Souvik Chattopadhaya†, and Shao Q. Yao†‡†
Department of Biological Sciences, ‡Department of Chemistry, National University of Singapore, 3 Science
Drive 3, Singapore 117543
OIV-2

New Chemical Genetic Analysis of Cellular Signal Transduction――
Kevan M. Shokat
Department of Cellular and Molecular Pharmacology, UCSF; Department of Chemistry, UC Berkeley

24

Coffee Break (16:20-16:40)
OIV-3

OIV-4

(Chairpersons: Kohei Tsumoto and Takeshi Tsumuraya)
Endogenous Gene Regulation with Polydactyl Zinc Finger Transcription――
24
Factors
Carlos F. Barbas, III
The Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps ResearchInstitute, La Jolla, CA, USA, 92037.
Searching Sequence Space for Protein Catalysts――
24
Donald Hilvert
Laboratorium für Organische Chemie, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093
Zurich, Switzerland18

生命化学研究レター No. 13 (2004. January) 19

Friday, December 5
Session V. Technology Innovation in Biomolecular Chemistry
9:00-13:00 (Chairperson: Shigeori Takenaka)
OV-1 The Chemical Biology of Protein Splicing
Tom W. Muir
The Rockefeller University, USA

Proceedings Abstract
――
24

OV-2

Tagged Small Molecule Library Approach to Facilitated Chemical Genetics
302
Sonya M. Khersonsky, Da-Woon Jung, Jae Wook Lee, Daniel P. Walsh, and Young-Tae Chang
Department of Chemiry, New York University, New York, NY 10003, USA

24

OV-3

Novel Fabrication Method for Protein Chip: Electrospray Deposition of――
24
Protein onto MALDI-TOF-MS Sample Plate
Hisashi Arikuni
Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan, Yokohama Laboratory,
Biomarker Science Co.,Ltd, Leading Venture Plaza 409, 75-1 Ono-cho, Tsurumi-ku, Yokohama, 230-0046,
Japan
Coffee Break (10:40-10:50)

OV-4(Chairperson: Young-Tae Chang)Surface and Micro Bead Chemistry for Successful Peptide Array Synthesis
308
24
and Protein Identification
Dong-Sik Shin†, Woo-Jae Chung†, Do-Hyun Kim†, Kook-Nyung Lee‡, Min-Su Kim‡, Suhyung Cho§,
Yong-Kweon Kim‡, Byung-Gee Kim† §, and Yoon-Sik Lee††
School of Chemical Engineering, Seoul National University, Seoul 151-744, Korea, ‡School of ElectricalEngineering & Computer Science, Seoul National University, Seoul 151-744, Korea, §Interdisciplinary
Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul 151-744, Korea

OV-5

From the DNA Sensor to the Future DNA Chip
312
24
Shigeori Takenaka
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka-City, Fukuoka 812-8581, Japan

OV-6

Nano-Biodevice in the Post-Genome Sequencing Era
316
24
Yoshinobu Baba†‡†
Department of Medicinal Chemistry, The University of Tokushima, CREST, JST, Tokushima, Japan,‡
Single-Molecule Bioanalysis Laboratory, National Institute of Advanced Industrial Science andTechnology(AIST), Takamatsu, Japan19

生命化学研究レター No. 13 (2004. January) 20

POSTER PROGRAM
Wednesday, December 3 (odd-numbered), Thursday, December 4 (even-numbered)
Session I. Functional DNA/RNA
13:00-15:00 Reception Hall (B)Proceedings
PI-01
In Vitro Selection of RNA Aptamers That Inhibit the Amyloid-Fibril Formation of Ab
20
Kosuke Tada, Tsuyoshi Takahashi, and Hisakazu Mihara
Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute ofTechnology, Yokohama 226-8501, Japan
PI-02

In Vitro Selection of Four Way Junction DNA on a QCM
22Tomomitsu Ozeki, Hiroyuki Furusawa, and Yoshio Okahata
Department of Biomolecular Engineering , Tokyo Institute of Technology, Yokohama, Kanagawa 226-8501,
Japan, and CREST, Japan Science and Technology Corporation

PI-03

In Vitro Selection of Saccharide-Modified DNA That Bind to Lectin
24
Masayuki Matsui, Akiko Onizawa, and Yasuhito Ebara
Graduate School of Cultural Studies and Human Science, Kobe University, Kobe, Hyogo 657-8501, Japan

PI-04

Induction and Inhibition of P. aeruginosa Quorum Sensing by Synthetic Autoinducer
26
Analogs
Hiroaki Suga§†, Kristina Smith§, and Yigong Bu§§
Departments of Chemistry and Biological Sciences, University at Buffalo, The State University of New York,
Buffalo, NY 14260-3000, USA, †Research Center for Advanced Science and Technology, University of Tokyo,
4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan

PI-05

A Ribozyme Displaying Alcohol Dehydrogenase Activity
28
Hiroaki Suga†‡, Shinya Tsukiji§, Swetansu B. Pattnaik‡†
Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku,
Tokyo 153-8904, Japan, ‡Department of Chemistry, University at Buffalo, The State University of New York,
Buffalo, NY 14260-3000, USA, §Department of Chemistry and Biotechnology, School of Engineering,
University of Tokyo, 7-3-1 Hongo, Tokyo 113-8656, Japan

PI-06

A Versatile Ribozyme for tRNA Aminoacylation
30
Hiroshi Murakami†‡, Hirohide Saito†§, and Hiroaki Suga†‡†
Department of Chemistry, University at Buffalo, State University of New York, Buffalo, NY 14260-3000,
USA, Current address: ‡Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1
Komaba, Meguro-ku, Tokyo, 153-8904, Japan, §Cancer Institute, Japanese Foundation for Cancer Research,
1-37-1 Kami-ikebukuro, Toshima-ku, Tokyo, 170-8455, Japan

PI-07

Engineering of tRNA for Incorporation of Nonnatural Amino Acids into Proteins32
Hikaru Taira†, Masaharu Fukushima†, Takahiro Hohsaka§, and Masahiko Sisido††
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1
Tushimanaka, Okayama 700-8530, Japan, §School of Materials Science, Japan Advanced Institute of Scienceand Technology, 1-1 Asahidai, Tatunokuchi, Isikawa 923-1292, Japan

PI-08

A Nano-Circular Single-Stranded DNA Works as a Novel Tool for Cell-Free Peptide
34
Synthesis and Detection of SNPs
Tatsuo Ohmichi†§, Aki Takashima‡, and Naoki Sugimoto†‡†
High Technology Research Center, and ‡Department of Chemistry, Faculty of Science and Engineering,
Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan, §I.S.T Corporation, 13-13-5
Ichiriyama, Otsu, Shiga 520-2153, Japan

PI-09

Nonenzymatic Organic Synthesis on a Single Strand DNA by Artificial tRNA36
Kunihiro Kaihatsu, Shin-ichi Ueji, and Yasuhito Ebara
Graduate School of Cultural Studies and Human Science, Kobe University, Kobe, Hyogo 657-8501, Japan

PI-10

A Thermal RNA Functional Switch
Kazuo Harada, Xianglan Li, and Koh Kobayashi
Department of Life Science, Tokyo Gakugei University, Koganei, Tokyo 184-8501, Japan20

38

生命化学研究レター No. 13 (2004. January) 21
PI-11

Supramolecular Complex Formation with β-Cyclodextrin and Adamantyl
40
Naphthalene Diimide Bound to Double Stranded DNA by Threading Intercalation
Shinobu Sato†, Takahiko Nojima†, Hiroki Kondo‡, and Shigeori Takenaka††
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka 812-8581, Japan, ‡Department of Biochemical Engineering and Science, Faculty of Computer
Science and Systems Engineering, Kyushu Institute of Technology, Kawazu 680-4, Iizuka, Fukuoka 820-8502,
Japan

PI-12

Peptide Ribonucleic Acids (PRNAs): Novel Strategy for Active Control of DNA
42
Recognition by External Factors
Takehiko Wada†, Hirofumi Sato†, Yusuke Hashimoto†, and Yoshihisa Inoue†§†
Department of Molecular Chemistry, Graduate School Engineering, Osaka University, Suita, Osaka
565-0871, Japan, ‡CREST, Japan Sciences and Technology Corporation (JST). Shomachi, Tokushima
770-8505, Japan, §Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The Universityof Tokushima, Shomachi, Tokushima 770-8505, Japan

PI-13

Conformation and Recognition Control of alpha-Peptide Ribonucleic Acid by
46
External Factors
Mayuko Kikkawa†, Hirofumi Sato†, Takehiko Wada†, and Yoshihisa Inoue†‡†
Department of Molecular Chemistry, Graduate School of Engineering, Osaka University, Yamada-oka, Suita
565-0871, Japan, ‡ICORP Entropy Control Project, JST, 4-6-3 Kamishinden, Toyonaka 565-0085, Japan

PI-14

Conformation and Recognition Control of Peptide Ribonucleic Acid Derivatives by
48
External and Internal Factors
Tetsuya Hirose†, Hirofumi Sato†, Takehiko Wada†, and Yoshihisa Inoue†‡†
Department of Molecular Chemistry, Graduate School of Engineering, Osaka University, Yamada-oka, Suita
565-0871, Japan, ‡ICORP Entropy Control Project, JST, 4-6-3 Kamishinden, Toyonaka 565-0085, Japan

PI-15

Synthesis and Characterization of Viologen-Tethered Peptide Nucleic Acid (VPNA) or
50
Diazapyrene-Tethered PNA (DPNA)
Atsushi Hida, Hisafumi Ikeda, and Yusin Nakamura
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo
University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan

PI-16

Bis-Pyrene Modified Oligonucleotides Provide a Useful Probe for the Detection of52
Single Base Mismatches Based on Polycation Stimulated DNA Strand Exchange
Reaction
Kazushige Yamana†‡, Yudai Fukunaga†, Yusuke Ohtani†, Hidehiko Nakano†, Won Jong Kim¶, Toshihiro
Akaike¶, and Atsushi Maruyama¶§†
Department of Applied Chemistry, Himeji Institute of Technology, 2167 Shosha, Himeji 671-2201, Japan,‡
SOST of JST, ¶Department of Biomolecular Engineering, Tokyo Institute of Technology, Midori, Yokohama
226-8501, Japan, and §PRESTO of JST

PI-17

Relationship between Structure of Cationic Groups of Dendritic Poly(L-lysine) and54
Gene Transfection Ability into Cells
Takuro Niidome†, Tatsuya Okuda‡, and Haruhiko Aoyagi‡†
Department of Materials Science, Graduate School of Science and Technology, Nagasaki University,
Nagasaki 852-8521, Japan, ‡Department of Marine Science and Technology, Graduate School of Science andTechnology, Nagasaki University, Nagasaki 852-8521, Japan

PI-18

Rapid and Simple DNA Mismatch Detection Using Artificial Nucleic Acid Chaperones
56
Atsushi Maruyama†‡, Won Jong Kim‡, Yuichi Sato†, and Toshihiro Akaike‡†
Presto, JST, ‡Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho,
Midori, Yokoihama 226-8501, Japan

PI-19

Synthesis of Bis-Naphthalene Diimide and Its Interaction with Double Stranded DNA
58
Toyofumi Nagamatsu, Keiichi Ohtsuka, Takahiko Nojima, and Shigeori Takenaka
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka-City, Fukuoka, 812-8581, Japan

PI-20

Electrochemical Study of Ferrocenylnaphthalene Diimide on the DNA-ImmobilizedGold Electrode21

60

生命化学研究レター No. 13 (2004. January) 22
Masaharu Komatsu†, Shinobu Sato†, Takahiko Nojima†, Hiroki Kondo‡, and Shigeori Takenaka†
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka 812-8581, Japan, ‡Department of Biochemical Engineering and Science, Faculty of Computer
Science and Systems Engineering, Kyushu Institute of Technology, Kawazu 680-4, Iizuka, Fukuoka 820-8502,
Japan
†

PI-21

Synthesis of Poly-Acridine Orange Peptides Aiming at a Fluorescent Reagent with
62
High Preference for Double Stranded DNA
Yutaka Sakakibara, Hiroyuki Ueyama, Keiji Mizuki, Satoshi Fujii, Takahiko Nojima, and Shigeori Takenaka
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka-City, Fukuoka 812-8581, Japan

PI-22

Interaction between Conformationally-Restricted Oxy-Peptide Nucleic Acids/DNA
64
Hybrids and a Cyanine Dye
Mizuki Kitamatsu, Naoki Ototake, Takashi Nakai, Mamoru Saito, Sayaka Nakamura, Tomoyuki Okada, andMasahiko Sisido
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushimanaka
3-1-1, Okayama 700-8530, Japan

PI-23

Molecular Design, Chemical Synthesis and Evaluation of Cyclic Trimers of Thiazoles66
as Novel Human Telomerase Inhibitors
Ryoko Imagawa, Shuichi Matsumura, and Kazunobu Toshima
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi,
Kohoku-ku, Yokohama 223-8522, Japan

PI-24

Self-Organized Nanostructures in Individual Giant DNA Molecules Controlled with
68
Polyamine Surfactants and Nanogels
Naomi Miyazawa†, Takahiro Sakaue‡, Kenichi Yoshikawa‡, and Kazunari Akiyoshi††
Institute of Biomaterials and Bioengineering Tokyo Medical and Dental University, 2-3-10
Kanda-Surugadai Chiyoda-ku Tokyo 101-0062, Japan, ‡Department of Physics, Graduate School of Science,
Kyoto University, Kyoto 606-8502, Japan

PI-25

Chiral Differentiation in DNA Compaction Induced by Tripeptides
70
Michiko Ito†, Shizuaki Murata‡, and Kenichi Yoshikawa§†
CREST of Japan Science and Technology Corporation (JST), Chikusa, Nagoya, 464-8601, Japan, ‡Graduate
School of Environmental Studies, c/o School of Informatics and Sciences, Nagoya University, Chikusa,
Nagoya, 464-8601, Japan, §Department of Physics, Graduate School of Science, Kyoto University, Kyoto,606-8504, Japan

PI-26

Application of Novel Functionalized Peptide Nucleic Acid Probes Having
72
Cell-Membrane Permeability
Madoka Tonosaki, Hisafumi Ikeda, Akinori Sugiyama, Fumio Tashiro, and Yushin Nakamura
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo
University of Science, Noda, Chiba 278-8510, Japan

PI-27

An Intracellular Signal-Responsive Artificial Gene Carrier for Novel Cell-Specific76
Gene Expression
Kenji Kawamura, Tatsuhiko Sonoda, Jun Ohishi, Masaharu Murata, and Yoshiki Katayama
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 6-10-1 Hakozaki,
Higashi-ku, Fukuoka 812-8581, Japan

PI-28

Biodegradable Gene Carrier Based on b-(1,3)-D-Glucan: Schizophyllan
78
Takeshi Nagasaki†, Masaya Hojo†, Atsushi Uno†, Kazuya Koumoto‡, Masami Mizu‡, Kazuo Sakurai‡, andSeiji Shinkai§†
Department of Applied & Bioapplied Chemistry, Graduate School of Engineeirng, Osaka City University,
Sugimoto, Sumiyoshi, Osaka 558-8585, Japan, ‡Department of Chemical Process & Enviroments, The
University of Kitakyushu, Hibikino, Kitakyushu, Fukuoka 808-0135, Japan, §Department of Chemistry &Biochemistry, Graduate School of Engineering, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan

PI-29

Effect of the Substituents of Naphthalene Diimide on Its Threading Intercalation into
the DNA Duplex
Satoshi Kumamoto, Shinobu Sato, Takahiko Nojima, and Shigeori Takenaka

2282

生命化学研究レター No. 13 (2004. January) 23
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka-City, Fukuoka 812-8581, Japan

Session II. Cell Function of Macromolecules
13:00-15:00 Reception Hall (B)Proceedings
PII-01A Sugar Cylinder – Molecular Design and Functions
102
Keigo Aoi†‡, Midori Okazaki†, and Masahiko Okada§†
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601,
Japan, ‡PRESTO, JST, §College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho,Kasugai, Aichi 487-8501, Japan
PII-02Synthesis of Peptidoglycan Partial Structures for Elucidation of the Mechanism of Its
104
Immunostimulation
Yukari Fujimoto, Seiichi Inamura, Akiko Kawasaki, Osamu Kubo, Koichi Fukase, and Shoichi Kusumoto
Department of Chemistry, Graduate School of Science, Osaka University Toyonaka, Osaka 560-0043, Japan

PII-03

Lectin-Induced Agglutination of Liposomes Composed of Synthetic Glycolipid
106
Analogues Possessing an Oligomethylene Spacer
Yasuo Azefu†, Hitoshi Tamiaki†, Reiko Sato‡, and Kazunori Toma‡†
Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University,
Kusatsu, Shiga 525-8577, Japan, ‡The Noguchi Institute, Itabashi, Tokyo 173-0003, Japan

PII-04Glycosylamidines as Potent and Selective Glycosidase Inhibitors
108
Masahiro Kato†, Jun Hiratake†, Masayasu Takada‡, Koichi Ogawa‡, and Kanzo Sakata††
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan, ‡Research Institute, Nihon
Shokuhin Kako Co., Ltd., 30 Tajima, Fuji, Shizuoka 417-8530, Japan

PII-05

Direct Monitoring of Glucan Hydrolysis by Glucoamylase on a Quartz-Crystal
110
Microbalance
Toshiaki Mori, Hidekazu Nishino, Takanori Nihira, and Yoshio Okahata
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Kanagawa 226-8501,
Japan, and CREST, Japan Science and Technology Corporation

PII-06Functions of Carbohydrate-Binding Peptides Selected from Phage-Displayed Peptide
112
Library
Teruhiko Matsubara†, Takao Taki‡, and Toshinori Sato††
Department of Biosciences and Informatics, Keio University, Yokohama 223-8522, Japan, ‡Molecular
Medical Science Institute, Otsuka Pharmaceutical Co. Ltd., Tokushima 771-0192, Japan

PII-07

Glyco-Helix: Specific Calcium-Lactose Interactions Induce a Re-Organization of
114
Lactose-Clusters of the Multi-Glycosylated Peptide
Teruaki Hasegawa†, and Tomikazu Sasaki‡†
Department of Chemistry, Graduate School of Engineering, Kyushu University, Hakozaki, 6-10-1, Fukuoka
812-8581, Japan, ‡Department of Chemistry, University of Washington, Seattle, WA 98105, USA.

PII-08Quantitative Proteome Analysis of Yeast by Two-Dimensional Differential Gel
116
Electrophoresis
Yi Hu†, Grace Y. J. Chen†‡, and Shao Q. Yao†‡†
Department of Biological Sciences, ‡Department of Chemistry, National University of Singapore, 3 Science
Drive 3, Singapore 117543

PII-09Gangliosides Assist for Constructing Liposomal Nanotube Network
118
Shin-ichiro M. Nomura†‡, Yumi Mizutani†§, Kimio Kurita§, and Kazunari Akiyoshi††
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo
101-0062, JAPAN, ‡Research Fellow of the JSPS, §Department of Science and Technology, Nihon University

PII-10Over-Expression of Ganglioside GM3 Suppresses Lung Metastatic Potential of Mouse
120
B16 Melanoma Cells Accompanied by Alterations in Spreading and Adhesion
Peixing Wu†, Tomoko Nomura‡, Hiroshi Koide§, Toshinori Sato†, Sadako Yamagata¶, and Tatsuya Yamagata¶23

生命化学研究レター No. 13 (2004. January) 24†

Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama
223-8522, Japan, ‡Tochigi Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai-machi,
Haga-gun, Tochigi 321-3497, Japan, §Graduate School of Medical Sciences, Kanazawa University,
Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan, ¶Department of Cell Biology and Glycobiology,
Shenyang Pharmaceutical University, 103 WenHua Road, Shenyang 110016, P. R. China
PII-11

Peptide-Mediated Delivery of Bioactive Molecules into Living Cells
Shiroh Futaki
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan

122

PII-12

Design, Synthesis and Photochemical Properties of Caged Compounds of Lipid
124
Mediators
Toshiaki Furuta, Keiko Nishiyama, Asako Manabe, Mutsumi Fukuoka, and Michiko Iwamura
Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi, Chiba 274-8510,
Japan

PII-13Creation of Multicellular Architecture with Bioinspired Organic-Inorganic
126
Nanohybrid "Cerasome"
Mineo Hashizume, Masashi Otsuki, Masayoshi Mabuchi, Atsushi Ikeda, and Jun-ichi Kikuchi
Graduate School of Materials Science, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama,
Ikoma, Nara 630-0192, Japan

PII-14

New Method for Evaluation of Intracellular Protein Kinase Signal Using Mass
128
Spectrometry
Yoshiki Katayama†, Shuhei Shigaki†, Takeyuki Nagashima‡, Osamu Okitsu‡, Tatsuhiko Sonoda†, Masaharu
Murata†, and Yasuhiro Kita‡†
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 6-10-1 Hakozaki,
Higashi-ku, Fukuoka 812-8581, Japan, ‡Advanced Technology Platform Research Laboratory, Fujisawa
Pharmaceutical Co. Ltd., 5-2-3 Thokoudai, Tsukuba 300-2698, Japan

PII-15

Quorum Sensing Control using Autoinducer Analogues in Gram-Negative Bacteria
130
Tsukasa Ikeda†, Takenori Ishida‡, Tomohiro Morohoshi†, Norihiro Kato†, Hirofumi Ikeshoji‡, Junich Kato‡,and Hisao Ohtake‡†
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya
321-8585, Japan, ‡Department of Molecular Biotechnology, Graduate School of Advanced Science andMatter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan

PII-16Development of Novel Fluorescence Resonance Energy Transfer Probes for
132
Ratiometric Imaging of Protein Tyrosine Phosphatase Activity
Hideo Takakusa†, Kazuya Kikuchi†‡, and Tetsuo Nagano††
Graduate School of Pharmaceutical Sciences, University of Tokyo, Hongo Bunkyo-ku Tokyo, 113-0033,
Japan, ‡Presto, JST Corporation, Kawaguchi Saitama, Japan

PII-17

Strong Induction of Apoptosis of T Cell Lines by Conger Eel Galectins
134
Tomohisa Ogawa†, Shintarou Yonemaru†, Takako Naganuma†, Jun Hirabayashi‡, Ken-ichi Kasai‡, and Koji
Muramoto††
Graduate School of Life Sciences, Tohoku University, Sendai 981-8555, Japan,‡Faculty of Pharmaceutical
Sciences, Teikyo University, Sagamiko, Kanagawa199-0195, Japan

Session III. Metals in Chemical Biology
13:00-15:00 Hallway from the Conference center to the Hotel
Proceedings
PIII-01 A Study of the Effect of Metal Ion on the DNA Replication Process from the Viewpoint
168
of Molecular Clustering in a Solution
Shunsuke Mochizuki, and Akihiro Wakisaka
Environmental Molecular Science Group, Institute for Environmental Management Technology, National
Institute of Advanced Industrial Science and Technology, 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan
PIII-02

Artificial Metallo-DNA: Structural Control and Discrete Metal Arrays by
Metal-Mediated Base Pairing
Kentaro Tanaka†‡, Atsushi Tengeiji†, Tatsuhisa Kato§, Namiki Toyama§, and Mitsuhiko Shionoya†

24

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生命化学研究レター No. 13 (2004. January) 25†

Department of Chemistry, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo
113-0033, Japan, ‡PRESTO, Japan Science and Technology Agency, §Institute for Molecular Science,
Myodaiji, Okazaki 444-8585, Japan
PIII-03

Molecular Design of Functional Peptides by Utilizing Unnatural Amino Acids:
172
Toward Artificial and Photo-Functional Protein
Hitoshi Ishida, Masato Kyakuno, and Shigero Oishi
Department of Chemistry, School of Science, Kitasato University, Kitasato, Sagamihara, Kanagawa
228-8555, Japan

PIII-04

The Orientation and Dipyridyl-Binding Property of Zinc and Cobalt Porphyrins
176
Linked to the Cyclic Peptide Gramicidin S
Toru Arai†, Koji Araki†, Tamaki Kato‡, and Norikazu Nishino‡†
Faculty of Engineering, Kyushu Institute of Technology, Tobata, Kitakyushu 804-8550, Japan, ‡Graduate
School of Life Science and Systems Engineering, Kyushu Institute of Technology, Wakamatsu, Kitakyushu
808-0196, Japan

PIII-05

Self-Assembly and Organization of Metalloporphyrin Dyes Assisted by an Artificial
178
LH Model Polypeptide
Keiji Yamashita, Yuzo Ohno, Mituhiro Ohta, Takehisa Dewa, and Mamoru Nango
Department of Applied Chemistry, Nagoya Institute of Technology, Gokisocho, Showa-ku, Nagoya, 466-8555,
Japan

PIII-06

Light-Harvesting Arrays Comprised of a Porphyrin Bearing Multiple
180
Perylene-Monoimide Accessory Pigments
Kin-ya Tomizaki†, Robert S. Loewe†, Christine Kirmaier‡, Jennifer K. Schwartz‡, Jennifer L. Retsek‡, David
F. Bocian§, Dewey Holten‡, and Jonathan S. Lindsey††
Department of Chemistry, North Carolina State University, Raleigh, NC 27695-8204, USA, ‡Department ofChemistry, Washington University, St. Louis, MO 63130-4899, USA, §Department of Chemistry, University ofCalifornia, Riverside, CA 92521-0403, USA

PIII-07

Self-Aggregation of Synthetic Chlorophylls Possessing an Isocyclic Six-Membered
182
Ring
Hitoshi Tamiaki, and Yasuhide Shimamura
Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University,
Kusatsu, Shiga 525-8577, Japan

PIII-08

Diastereoselective Self-Aggregation of Synthetic Zinc Bacteriochlorophyll-d Homologs

184

Hitoshi Tamiaki, Hiroyuki Kitamoto, Akiyoshi Nishikawa, Takuya Hibino, and Reiko Shibata
Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University,
Kusatsu, Shiga 525-8577, Japan
PIII-09

Stereochemical Differentiation of Dipeptides by Use of Chiral Gadolinium(III)188
Porphyrinate as a Circular Dichroism Probe
Hitoshi Tamiaki†, Satomi Unno†, Eiji Takeuchi†, Satoshi Shinoda‡, and Hiroshi Tsukube‡†
Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University,
Kusatu, Shiga, 528-577, Japan, ‡Department of Chemistry, Graduate School of Science, Osaka City
University, Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan

PIII-10

Peroxidase and Peroxygenase Activities of a Myoglobin Reconstituted with an Iron
190
Porphycene
Dai Murata, Hideaki Sato, Takashi Hayashi, and Yoshio Hisaeda
Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka
812-8581, Japan

PIII-11

Bioencapsulation for in situ Synthesis of Metal Nanocompounds and Regulation of
Catalytic Reactions
Takafumi Ueno†, Satoshi Abe‡, Masataka Ohashi§, and Yoshihito Watanabe‡

25

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生命化学研究レター No. 13 (2004. January) 26†

Research Center for Materials Science and ‡Department of Chemistry, Graduate School of Science, Nagoya
University, Nagoya 464-8602, Japan, §Department of Structural Molecular Science, The Graduate University
for Advanced Studies, Okazaki 444-8585, Japan
PIII-12Anti-Allergic Drug Discovery Based on the Structure of Human Prostaglandin D
194
Synthetase with the Metal Activation Mechanism
Tsuyoshi Inoue†‡, Kousuke Aritake§, Hiroyoshi Matsumura†, Yasushi Kai†, and Yoshihiro Urade§†
Department of Materials Chemistry, Graduate School Engineering, Osaka University, Suita, Osaka
565-0871, Japan, ‡PRESTO, Japan Science and Technology Corporation (JST), Kyoto 604-0847,
Japan, §Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan

PIII-13

196
Crystal Structures of Ferrous Form and CO-, CN--, and NO-Bound Forms of Rat
Heme Oxygenase (HO): Implications for Discrimination between CO and O2 in HO
Hiroshi Sakamoto†, Masakazu Sugishima‡, Keiichi Fukuyama‡, and Masato Noguchi††
Department of Medical Biochemistry, Kurume University School of Medicine, 67 Asahi-machi,
Kurume830-001, Japan, ‡Department of Biology, Graduate School of science, Osaka University, Toyonaka,
Osaka 560-0043, Japan

PIII-14Structural Difference between Holo- and Apo-Forms of Metal-Binding Protein of
198
Hematopoietic Prostaglandin D Synthetase
Tsuyoshi Inoue†‡, Yuji Kado†, Tomoyuki Doi†, Yousuke Okano†, Daisuke Irikura§, Hiroyoshi Matsumura†,
Yasushi Kai†, and Yoshihiro Urade§†
Department of Materials Chemistry, Graduate School Engineering, Osaka University, Suita, Osaka
565-0871, Japan, ‡PRESTO, Japan Science and Technology Corporation (JST), Kyoto 604-0847,
Japan, §Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan

PIII-15

Molecular Mechanism Responsible for pH-Modulation of Redox Potential of P.
200
Aeruginosa Cytochrome c-551
Shin-ichi J. Takayama†, Naoki Tachiiri†, Norifumi Terui†, Hajime Mita†, Yoshihiro Sambongi‡, Susumu
Uchiyama§, and Yasuhiko Yamamoto††Department of Chemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8571, Japan, ‡Graduate School of
Biosphere Science, Hiroshima Univ., Higashihiroshima, Hiroshima 739-8528, Japan, §Graduate School ofEngineering, Osaka University, Suita, Osaka 565-0871, Japan

PIII-16

Purification and Functional Analysis of Vanadium Nitrogenase Regulating Protein,
202
VnfA
Hiroshi Nakajima†, and Shigetoshi Aono‡†
Division of Chemistry, Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan,‡
Center for Integrative Bioscience, Institute of Molecular Science, Okazaki, Aichi 444-8585, Japan

PIII-17

DNA Binding Affinity of Artificial Zinc Fingers Having Additional Cys-X-Y-Cys
204
Sequence at N-Terminus or C-Terminus
Kenji Kaneko, Yuuki Nakamura, Naoto Shimadzu, Akira Onoda, and Takeshi Yamamura
Department of Chemistry, Faculty of Science, Tokyo University of Science, Shinjyuku-ku, Tokyo 162-8601,
Japan

PIII-18

A Novel Approach to an Artificial Nuclease Based on the Zinc Finger Motif
Akiko Nomura, and Yukio Sugiura
Institute for Chemical Research, Kyoto University, Uji Kyoto 611-0011, Japan

PIII-19

Synthesis and Structural Analysis of the Novel Zinc Finger Protein Having a Troponin
208
Site
Nozomi Arai†, Naoto Shimadzu†, Kenji Kaneko†, Takato Niki†, Akira Onoda†, Hitoshi Yamamoto‡, andTakeshi Yamamura††
Department of Chemistry, Faculty of Science, Tokyo University of Science, Shinjuku-ku, Tokyo 162-8601,
Japan, ‡Department of Macro Molecular Science Graduate School of Science Osaka University Toyonaka,
Osaka, 560-0043, Japan

206

Session IV. Protein-Protein Interaction
13:00-15:00 Hallway from the Conference center to the Hotel
PIV-01 A Photoaffinity Scanning System to Investigate the Client-Binding of the
C ll
S ifi Ch
HSP47

26

Proceedings
218

生命化学研究レター No. 13 (2004. January) 27
Collagen-Specific Chaperone HSP47
Takaki Koide†, and Yoshifumi Takahara††
Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, 2-1
Minamijosanjima-cho, Tokushima 770-8506, Japan
PIV-02

Small Catalytic Peptides That Operate via an Enamine Mechanism: Improving
222
Catalytic Activity and Substrate Specificity
Fujie Tanaka, Roberta Fuller, and Carlos F. Barbas, III
The Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps ResearchInstitute, 10550 North Torrey Pines Road, La Jolla, California 92037, U.S.A.

PIV-03

Expression and Application of Peptide Materials Containing Functionalizing
224
Sequence by the Rolling Circle Amplification
Cho Lay Sint†, Shintaro Himeno†, Iwao Yamane†, Yoko Terada†, Kazuyasu Konko‡, and Shokichi Ohuchi†‡†Department of Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu, 808-0196
Japan, ‡Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka
820-8502, Japan

PIV-04

Design and Synthesis of De Novo Peptide Probe for Sensing of Protein Kinase
226
Seiji Sakamoto, Chihiro Hayashi, and Kazuaki Kudo
Institute of Industrial Science, University of Tokyo, Komaba 4-6-1, Meguro-ku, Tokyo 153-8505, JAPANPIV-05Observation of a Specific Interaction between One Residue-Replaced Leucine-Zipper
232
PeptidesHiroyuki Furusawa, Hidefumi Ishii, Masami Ishii, and Yoshio Okahata
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Kanagawa 226-8501,
Japan, and CREST, Japan Science and Technology Corporation

PIV-06Novel Artificial Receptors and Chemosensors toward Phosphorylated Peptide in
234
Aqueous Solution
Akio Ojida†, Masa-aki Inoue†, Yasuko Mito-oka†, and Itaru Hamachi§‡††
Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka
812-8581, Japan, ‡Institute for Materials Chemistry and Engineering (IMCE), §PRESTO (Organization andFunction, JST)

PIV-07

Specific Interaction between Peptides Derived from Fusion and Transmembrane
Domains of Influenza HA2Eric A. B. Kantchev, Shu-fang Cheng, and Ding-kwo Chang
Institute of Chemistry, Academia Sinica, Nankang, Taipei 11529, Taiwan

PIV-08Interaction Analysis of Erythropoietin Receptor and EMP1 using Quartz-Crystal
238
Microbalance
Hideki Shigematsu†, Kouhei Tsumoto‡, Izumi Kumagai‡, and Yoshio Okahata††
Depertment of Biomolecular Engineeringy, Tokyo Institute of Technology, and CREST, Japan Sciences andTechnology Corporation (JST), Yokohama, Kanagawa 226-8501, Japan, ‡Depertment of MolecularEngineering Tohoku University, Sendai, Miyagi 980-8579, Japan

PIV-09An Oligopeptide Containing the C-terminal Sequence of RNase A Inhibits an RNA
240
Hydrolysis by RNase A
Shu-ichi Nakano†, Yuuki Uotani‡, and Naoki Sugimoto†‡†
High Technology Research Center, and ‡Department of Chemistry, Faculty of Science and Engineering,
Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan

PIV-10The Interaction Analysis between Human Interleukin 21 and Its Soluble Receptor
242
Koki Makabe†, Kouhei Tsumoto†, Ryutaro Asano†, and Izumi Kumagai††
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama
07, Aoba-ku, Sendai 980-8579, Japan

PIV-11

Non-Substrate Ligands for Carbonic Anhydrase VI in Submaxillary GlandAsako Watanabe†, Toshiho Nishita‡, and Isamu Matsumoto§

27

236

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生命化学研究レター No. 13 (2004. January) 28†

Course of Advanced Biosciences, Graduate School of Humanities and Sciences, Ochanomizu University,
2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan, ‡Laboratories of Veterinary Physiology 1, School of
Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan,§
Department of Chemistry, Faculty of Science, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo
112-8610, Japan
PIV-12Control of Lysozyme Activity by Weak Ultrasound Irradiation
246
Yu Hoshino, Takayoshi Kawasaki, and Yoshio Okahata
Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatuda Midori-ku,
Yokohama 226-8501, Japan

PIV-13g-Phosphono Derivatives of Glutamic Acid as Mechanism-Based Inhibitors of248
g-Glutamyltranspeptidase
Jun Hiratake†, Norihito Tachi†, Han Liyou†, Hideyuki Suzuki‡, Hidehiko Kumagai‡, and Kanzo Sakata††
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan, ‡Graduate School of
Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan

PIV-14

Correlation between Enzymatic Function, Sequence Similarity and Molecular
250
Fluctuation of Biotin Carboxylase
Satoshi Fujii†, Shigeori Takenaka†, Yuko Ito‡, Shinji Sueda‡, and Hiroki Kondo‡†
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka 812-8581, Japan, ‡Department of Biochemical Engineering and Science, Kyushu Institute ofTechnology, Kawazu 680-4, Iizuka 820-8502, Japan

PIV-15An Accurate Assignment of Protein Secondary Structure for the Protein Design
252
Kazuyasu Konko†, Hiroya Osoegawa‡, Tsutomu Hamada§, and Shokichi Ohuchi†‡†Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka 820-8502,
Japan, ‡Department of Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu
808-0196, Japan, §Pro Device, Co. Ltd., Fukuoka 819-0014 Japan

PIV-16

Synthesis-Based Approach Toward Direct Sandwich Immunoassay for Ciguatoxin
254
CTX3C
Takeshi Tsumuraya†, Hiroki Oguri‡, Megumi Maruyama‡, Hisatoshi Uehara‡, Yoko Nagumo‡, Masahiro
Hirama‡, and Ikuo Fujii††
Department of Bioorganic Chemistry, Biomolecular Engineering Research Institute, Suita, Osaka 565-0874,
Japan, ‡Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan

PIV-17

Selection and Characterization of Human Antibody Fragments against Biodegradable
256
Polymer
Hideki Watanabe†, Kouhei Tsumoto†, Seiichi Taguchi‡, Koichi Yamashita‡, Tadashisa Iwata‡, Yoshiharu
Doi‡, Hidemasa Kondo§, Yoshiyuki Nishimiya§, and Izumi Kumagai††
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai,
Miyagi 980-8579, Japan, ‡Polymer Chemistry Laboratory, RIKEN Institute, Wako-shi Saitama 351-0198,
Japan, §Institute for Biological Researches and Functions, National Institute of Advanced Industrial Scienceand Technology, Sapporo, Hokkaido 062-8517, Japan

PIV-18How Additives Influence the Refolding of Immunoglobulin-Folded Proteins in a
258
Stepwise Dialysis System
Mitsuo Umetsu†‡, Kouhei Tsumoto†, Masaki Hara†, Kumar Ashish†, Shuichiro Goda†, Tadafumi Adschiri‡,and Izumi Kumagai††
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aobayama
07, Aoba-ku, Sendai 980-8579, Japan, ‡Institute of Multidisciplinary Research for Advanced Materials,
Tohoku University, 2-1-1, Katahira, Aoba-ku, Sendai 980-8577, Japan

PIV-19Synthesis and Characterization of a Fluorescent Nonnatural Antibody Protein forReal Time Detection of a Target Molecule
Hiroyuki Hamada†, Hiroaki Shinohara‡, Takahiro Hohsaka§, and Masahiko Sisido†

28

260

生命化学研究レター No. 13 (2004. January) 29†

Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima-naka,
Okayama 700-8530, Japan, ‡Department of System Engineering of Material and Life Science, Faculty ofEngineering, Toyama University, Gofuku, Toyama 930-8555, Japan, §School of Materials Science andTechnology, Japan Advanced Institute of Science and Technology, Tatsunokuchi, Ishikawa 923-1292, Japan
PIV-20Solid-Phase Artificial Chaperone-Assisted Refolding Using b-Cyclodextrin Copolymer
262
Hydrogel Beads
Satoshi Yamaguchi, Shinya Tsukiji, Teruhisa Mannen, and Teruyuki Nagamune
Department of Chemistry & Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo
113-8656, Japan

PIV-21

ETB, an Epolactaene Derivative, Binds to Human Hsp60 and Inhibits the Chaperone
264
Activity
Yoko Nagumo†, Hideaki Kakeya†, Mitsuru Shoji‡, Yujiro Hayashi‡, and Hiroyuki Osada††
Antibiotics Laboratoy, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan, ‡Department of Industrial
Chemistry, Faculty of Engineering, Tokyo University of Science, Kagurazaka, Shinjuku-ku, Tokyo 162-8601,
Japan

PIV-22A Large-Size Host-Guest Chemistry Towards Bio-Nanomaterial Synthesis:
266
Chaperonin GroEL Complexed with Dendritic Molecule
Yoshio Goto†, Dong-Lin Jiang†, Takuzo Aida†, Mina Okochi‡, Masafumi Yohda‡, and Noriyuki Ishii§†
ERATO Nanospace Project, Japan Science and Technology Corporation, 2-41 Aomi, Koto-ku, Tokyo
135-0064, Japan, ‡Department of Biotechnology, Tokyo University of Agriculture and Technology,2-24-16
Nakacho, Koganei, 184-8588 Japan, §Advanced Institute for Science and Technology, 1-1-1 Higashi, Tsukuba
305-8566, Japan

PIV-23

Design of Artificial Chaperon by Nanogel Engineering
268
Yuta Nomura†, Yasuhiro Aoyama†, and Kazunari Akiyoshi‡†
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto
University, Yoshida-Hommachi, Sakyo-ku, Kyoto 606-8501, Japan, ‡Institute of Biomaterials andBioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo,
101-0062, Japan

PIV-24

Intein-Mediated Biotinylation of Proteins
270
Rina Y.P. Lue†, and Shao Q. Yao†‡†
Department of Biological Sciences, ‡Department of Chemistry, National University of Singapore, 3 Science
Drive 3, Singapore 117543

PIV-25

In Cell Protein Engineering: A Semi-Synthetic Approach to Modify Proteins in Living
274
Mammalian Cells
Shinya Tsukiji, Satoshi Goda, Shuji Takeda, and Teruyuki Nagamune
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo,
Bunkyo-ku, Tokyo, Japan 113-8656

PIV-26A Genetic Approach to Identifying Organelle-Localized Proteins
276
Takeaki Ozawa†, Yusuke Sako†, and Yoshio Umezawa††
Department of Chemistry, School of Science, The University of Tokyo, Hongo,Bunkyo-ku, Tokyo 113-0033,
Japan

PIV-27Site-Specific Covalent Labeling of Proteins Inside Live Cells Using Small Molecule
280
ProbesYeo Su-Yin Dawn†, and Yao Shao Qin†‡†
Department of Biological Science, ‡Department of Chemistry, National University of Singapore, 3 Science
Drive3, Singapore 117543, Singapore

PIV-28Intein-Mediated Conjugation of DNA to Proteins and Its Applications
282
Shuji Takeda†, Shinya Tsukiji†, Takeshi Wada†‡, Kazuhiko Saigo†‡, and Teruyuki Nagamune††
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo
113-8656, Japan, ‡Department of Integrated Biosciences, Graduate school of Frontier Sciences, The

29

生命化学研究レター No. 13 (2004. January) 30
University of Tokyo, Kashiwa, Chiba 277-8562, Japan
PIV-29

Self-Assembling Monolayer Formation of Glucose Oxidase and Cytochrome c284
Covalently Attached on Alkanethiol Monolayers on Gold
Koji Nakano†‡, Kenji Doi†, Kousuke Tamura†, Yosuke Katsumi†, and Masato Tazaki§†
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 6-10-1 Hakozaki,
Fukuoka 812-8581, Japan, ‡“Organization and Function”, PRESTO, Japan Science and Technology
Corporation, Honcho 1-8-4, Kawaguchi 332-0012, Japan, §General Education, Faculty of Engineering, Sojo
University, Ikeda 4-22-1, Kumamoto 860-0082, Japan

PIV-30Dendrimer Induced Acceleration of Enzymatic Degradation of Polycaprolactone
286
Keigo Aoi†‡, Hiromi Aoi†§, Rie Kawahito†, Hajime Ohtani§, and Hiroaki Sato¶†
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601,
Japan, ‡PRESTO, JST, §Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya
464-8603, Japan, ¶Institute for Environmental Management Technology, National Institute of Advanced
Science and Technology (AIST), Tsukuba 305-8569, Japan

PIV-31Equipment of Artificial Receptor with a Lectin by P-PALM for Construction of
288
Fluorescent Oligosaccharide -Biosensors
Eiji Nakata†, Tsuyoshi Nagase†, Seiji Shinkai†, and Itaru Hamachi†‡§†
Department of Chemistry and Biochemistry, Graduate School of Engineering,Kyushu University, Fukuoka
812-8581, Japan, ‡PRESTO (Organization and Function), JST, §Institute for Materials Chemistry andEngineering (IMCE)

PIV-32

Photo-Responsive Property of Semi-Synthetic RNase A by Peptide Backbone
290
Engineering
Noriyuki Kasagi†, Takashi Hiraoka†, and Itaru Hamachi†‡†Department of Chemistry and Biochemistry, Graduate School of Engineering,Kyushu University, Fukuoka
812-8581, Japan, ‡Institute for Materials Chemistry and Engineering (IMCE)

PIV-33Synthesis and Characterization of Mutants of Streptavidin in Which a Nonnatural
292
Amino Acid Carrying Ferrocenyl Group Was Incorporated at a Specific Site
Akira Fujii†, Hiroaki Shinohara‡, Takahiro Hohsaka§, and Masahiko Sisido††
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima-naka,
Okayama 700-8530, Japan, ‡Department of System Engineering of Material and Life Science, Faculty ofEngineering, Toyama University, Gofuku, Toyama 930-8555, Japan, §School of Materials Science andTechnology, Japan Advanced Institute of Science and Technology, Tatsunokuchi, Ishikawa 923-1292, Japan

PIV-34

In Vitro Engineering of GFP Mutants Containing Nonnatural Amino Acids at Tyr66
294
Position
Daisuke Kajihara†‡, Takahiro Hohsaka‡, and Masahiko Sisido††
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1
Tsushimanaka, Okayama 700-8530, Japan, ‡School of Materials Science, Japan Advanced Institute of Scienceand Technology, 1-1 Asahidai, Tatsunokuchi, Ishikawa 923-1292, Japan

PIV-35Photoswitching of Protein Activity by Position-Specific Incorporation of a
296
Photoisomerizable Nonnatural Amino Acid into Proteins
Norihito Muranaka†‡, Takahiro Hohsaka‡, Taishi Manabe†, Kaiyo Maeda†, and Masahiko Sisido††
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1
Thushimanaka, Okayama 700-8530, Japan, ‡School of Materials Science, Japan Advanced Institute of
Science and Technology, 1-1 Asahidai, Tatsunokuchi, Ishikawa 923-1292, Japan

Session V. Technology Innovation in Biomolecular Chemistry
13:00-15:00 Hallway from the Conference center to the Hotel
Proceedings
PV-01
The SPR Sensor Detecting Mismatch DNAs
320
Kazuhiko Nakatani†‡, Akio Kobori‡, Shinya Hagihara†, Hitoshi Suda†, Yuki Goto†, and Isao Saito††
Department of Synthetic Chemistry, Graduate School Engineering, Kyoto University, Kyoto, Kyoto‡
615 8510 J
PRESTO JS
dT h lC30

生命化学研究レター No. 13 (2004. January) 31
615-8510, Japan, ‡PRESTO, Japan Science and Technology Corporation
PV-02

Novel Immobilization Technique of Single-Stranded DNA Molecules on a Glass
322
Surface for Direct Analysis of DNA Polymerase Activity
Shun-ichi Matsuura†, Hiroshi Naito‡, Kei-ichi Takata§, Yoshiyuki Mizushina¶, Yasufumi Murakami†, Kengo
Sakaguchi§, Shinji Katsura‡, and Akira Mizuno‡†
Department of Biological Science & Technology, Tokyo University of Science, Yamazaki, Noda, Chiba
278-8510, Japan, ‡Department of Ecological Engineering, Toyohashi University of Technology,
Tempaku-cho, Toyohashi, Aichi 441-8580, Japan, §Department of Applied Biological Science, Tokyo
University of Science, Yamazaki, Noda, Chiba 278-8510, Japan, ¶Department of Nutritional Science,
Kobe-Gakuin University, Ikawadani-cho, Nishi-ku, Kobe, Hyogo 651-2180, Japan

PV-03

Simple Biosensing for a Target Oligonucletide by Fluorescence Measurement Using
326
Evanescent Wave
Hiroaki Shinohara†, Yasutaka Okamoto‡, and Masahiko Sisido‡†
Department of System Engineering of Materials and Life Science, Faculty of Engineering, Toyama
University, Gofuku,Toyama 930-8555, Japan, ‡Department of Bioscience and Biotechmology, Faculty ofEngineering, Tsushima-naka, Okayama 700-8530, Japan

PV-04

Development of a New Class of Molecular Beacons Utilizing a Switching between
328
Pyrene Monomer and Excimer Emissions: Highly Sensitive DNA Probes
Kazuhisa Fujimoto‡, Hisao Shimizu‡, and Masahiko Inouye†‡†
PRESTO, Japan Science and Technology Corporation (JST), ‡Faculty of Pharmaceutical Sciences, Toyama
Medical and Pharmaceutical University, Sugitani Toyama 930-0194, Japan

PV-05

Toward the Construction of Universal Small-Molecule Microarrays: Functional
330
Group Independently-Immobilized Arrays of Small Molecules
Naoki Kanoh†, Saori Kumashiro†, Siro Simizu†, Yasumitsu Kondoh‡, Satoru Hatakeyama‡, Hideo Tashiro‡,and Hiroyuki Osada††
Antibiotics Laboratory, Discovery Research Institute, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198,
Japan. ‡Advanced Engineering Center, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.

PV-06

Energy Transfer Behavior in Chromophore Array Constructed by DNA Assembly in
332
the Presence of Polycationic Graft Copolymer
Yuichi Ohya†, Naoto Ohe†, Tatsuto Ouchi†, Won Jon Kim‡, Toshihiro Akaike‡, and Atsushi Maruyama‡§†
Department of Applied Chemistry, Faculty of Engineering & High Technology Research Center, Kansai
University, Suita, Osaka 564-8680, Japan, ‡Department of Biomolecular Engineering, Tokyo Institute ofTechnology, 4259 Nagatsuda, Midoriku, Yokohama 226-8501, Japan, §Precursory Research for Embryonic
Science and Technology, Japan Science and Technology Corporation

PV-07

Synthesis of an Anthracene Derivative Carrying Fluorescein and Evaluation of Its
334
Specific Binding to Double Stranded DNA
Shin-ichiro Isobe, Keiji Mizuki, Satoshi Fujii, Takahiko Nojima, and Shigeori Takenaka
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka-City, Fukuoka 812-8581, Japan

PV-08

Novel Ferrocenylation Reagent of DNA for Electrochemical Gene Detection
336
Kosuke Mukumoto, Takahiko Nojima, and Shigeori Takenaka
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka-City, Fukuoka 812-8581, Japan

PV-09

Visualization of a DNA Hybrid on the DNA Microarray by FT-IR Microscopy
338
Keiji Mizuki, Takahiko Nojima, and Shigeori Takenaka
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka-City, Fukuoka 812-8581, Japan

PV-10

Design of Molecular Beacon Peptide Nucleic Acid (PNA) Arrays
340
Fumihiko Kitagawa, Hisafumi Ikeda, and Yushin Nakamura
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo
University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan31

生命化学研究レター No. 13 (2004. January) 32
PV-11

Construction of Protein-Detection Microarrays Using Structure-Based Peptide
344
Libraries
Kenji Usui†, Tetsunori Ojima†, Masato Suzuki†, Mizuki Takahashi†, Kiyoshi Nokihara‡, and Hisakazu
Mihara††Department of Bioengineering, Tokyo Institute of Technology, Yokohama 226-8501, Japan, ‡HiPep
Laboratories, Kyoto 602-8158, Japan

PV-12

Efficient Immobilization of Peptides Carrying a Disulfide Bond on a Solid Substrate
346
Ryo Kajiki, Keiichi Ohtsuka, Michinori Waki, Takahiko Nojima, and Shigeori Takenaka
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka-City, Fukuoka, 812-8581, Japan

PV-13

Fourier Transform Infrared (FT-IR) Coupled with Reflection Absorption
348
Spectroscopy (RAS) Detection of Carcinoembryonic Antigen (CEA) as a Cancer
Marker
Keiichi Ohtsuka, Takahiko Nojima, and Shigeori Takenaka
Department of Applied Chemistry, School of Engineering, Kyushu University, Hakozaki 6-10-1, Higashi-ku,
Fukuoka-City, Fukuoka, 812-8581, Japan

PV-14

Preparation of DNA-Modified Polystyrene Beads and Their Application to350
Colorimetry for Gene Detection
Shojiro Tanaka†, Toshihiro Ihara†, Yasushi Chikaura‡, and Akinori Jyo††
Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kumamoto University, 2-39-1
Kurokami, Kumamoto 860-8555, Japan, ‡Kumamoto Health Science University, 325 Izumimachi, Kumamoto
861-5598, Japan

PV-15

Single Nucleotide Mutation Assay Based on Salting-Out Techniques Using
352
DNA-Linked Colloidal Nanoparticles
Zhonglan Tang†‡, Yoshikuni Sato†, Tohru Takarada‡ and Mizuo Maeda†‡†
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 6-10-1 Hakozaki,
Higashi-ku, Fukuoka 812-8581, Japan, ‡Bioengineering Laboratory, The Institute of Physical and Chemical
Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

PV-16

Single DNA Molecule Processing on a Chip for Genome Analysis
354
Ken Hirano†, and Yoshinobu Baba†§†
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, andCREST of Japan Science and Technology Corporation (JST), Tokushima, Tokushima 770-8505, Japan,§
Single-Molecule Bioanalysis Laboratory, National Institute of Advanced Industrial Science and Technology(AIST), Takamatsu, Kagawa 761-0395, Japan

PV-17

Fast DNA Separation by Nanopillar Chip
358
Noritada Kaji†, Yojiro Tezuka‡, Yuzuru Takamura‡, Masanori Ueda†, Takahiro Nishimoto§, Hiroaki
Nakanishi§, Yasuhiro Horiike‡, and Yoshinobu Baba†¶†
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima,
CREST, Japan Science and Technology Corporation (JST), Tokushima 770-8505, Japan, ‡Department ofMaterial Engineering, The University of Tokyo, Tokyo 113-8656, Japan, §Technology Research Laboratory,
Shimadzu Corporation, Kyoto 619-0237, Japan, ¶Single-Molecule Bioanalysis Laboratory, National Instituteof Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan

PV-18

Microchip Separation of Specific DNA Fragments Using Mixed Polyethylene Oxide
360
SolutionsFeng Xu†‡, Mohammad Jabasini†, and Yoshinobu Baba†§†
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima,
CREST, JST, Japan, ‡Analytical Instruments Division, Shimadzu Corp., Kyoto, Japan, §Single-Molecule
Bioanalysis Laboratory, National Institute of Advanced Industrial Science and Technology, Takamatsu,
Japan

PV-19

Rapid Protein Detection on a Microchip without Denaturing
Mari Tabuchi†, Yasuhiro Kuramitsu‡, Kazuyuki Nakamura‡§, and Yoshinobu Baba†¶

32

364

生命化学研究レター No. 13 (2004. January) 33†

Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima,
Tokushima,770-8505, Japan and CREST, ‡Department of Biochemistry & Biomolecular Recognition,§
Central Laboratory for Biomedical Research and Education, Yamaguchi University School of Medicine,Ube, 755-8505, Japan, ¶Single-molecule Bioanalysis Laboratory, National Institute of Advanced Industrial
Science and Technology (AIST), Takamatsu,761-0935, Japan
PV-20

Thermal Lens Microscope for High-Sensitive Detection of DNA and Protein on a Chip
366
†
†
†
†‡
Hideya Nagata , Ken Hirano , Mari Tabuchi , and Yoshinobu Baba†
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima,
CREST, Japan Science and Technology Corporation (JST), Tokushima 770-8505, Japan, ‡Single-Molecule
Bioanalysis Laboratory, National Institute of Advanced Industrial Science and Technology (AIST),
Takamatsu, Japan

PV-21

The Novel Drug Development: Pyrazoles as Anti-Invasive drugs
60 (abstract)
Ajay Kumar†‡, Virinder S Parmar† ,Hriday N. Jha‡, Carl E Olsen§, and Marc M Mareel¶†
Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi-110 007, India, ‡Department ofChemistry, MMH College, Ghaziabad, UP, India, §Chemistry Department, Royal Veterinary and Agricultural
University, 40 Thorvaldsensvej, Frederiksberg C, DK-1871 Copenhagen, Denmark, ¶Laboratory ofExperimental Cancerology, Department of Radiotherapy, Nuclear Medicine and Experimental Cancerology,
University Hospital, De Pintelaan 185, B-9000 Gent, Belgium

PV-22

Profiling Kinase Activity Using Peptide-Based Microarrays
368
Mahesh Uttamchandani†, and Shao Q. Yao†‡†
Department of Biological Sciences, ‡Department of Chemistry, National University of Singapore, 3 Science
Drive 3, Singapore 117543

PV-23

Profiling Hydrolytic Enzymes Using Small Molecular Microarray
61 (abstract)
Zhu Qing†, Uttamchandani M, Chen, G. Y. J‡, Shao, and Q. Yao†‡†
Department of Chemistry, ‡Department of Biological Science, National University of Singapore, 3 Science
Drive 3, Singapore 117543

PV-24

Developing Novel Affinity Probes for the Affinity-based Profiling of Metalloproteases
63 (abstract)
Elaine W.S. Chan† and Shao Q. Yao†‡†
Department of Chemistry, ‡Department of Biological Science, National University of Singapore, 3 Science
Drive 3, Singapore 117543

33

生命化学研究レター No. 13 (2004. January) 34

プレ・ポストシンポジウム報告
The Ninth International Kyoto Conference on New Aspects of Organic Chemistry (IKCOC-9), which will beheld on November 10-14, 2003, at Kyoto Park Hotel, Kyoto, Japan"Control of Gene Expression by Peptide Nucleic Acids (PNA)"Peter E. Nielsen (Univ. Copenhagen)１１月２６日、大阪大学大学院理学研究科講演会"Control of Gene Expression by Peptide Nucleic Acids (PNA)"Peter E. Nielsen (Univ. Copenhagen)近畿化学協会合成部会ロボット・マイクロ合成研究会第 23 回研究会（第 11 回公開講演会(東京))The First Automated Oligosaccharide Synthesizer: Chemistry and Medical Advances
Prof. Peter H. Seeberger (ETH)

１２月１日、大阪大学大学院理学研究科講演会
The First Automated Oligosaccharide Synthesizer: Chemistry and Medical Advances
Prof. Peter H. Seeberger (ETH)生命化学研究レター No. 13 (2004. January) 35

ISBC2003 プレシンポジウム
4th Symposium on DNA / RNA / Protein for Nanotechnology 報告

科学研究費補助金特定領域研究「DNA／RNA の機能化を目指した化学的新展開」主催の第４回国際ミニシンポジウム、4th Symposium on DNA / RNA / Protein for Nanotechnology に対して生命科学研究会の協賛を頂き、ISBC2003 のレセプション当日 12 月 2 日に ISBC2003 と同じ兵庫県立夢舞台国際会議場１
Ｆアンフィシアターで開催しました。
科学研究費補助金特定領域研究「DNA／RNA の機能化を目指した化学的新展開」は、「核酸は高機能材料であり、化学的手法を活用することにより、さらなる高機能化が可能である」という視点から研究を推進しているプロジェクトです。社会の高度情報化と多様化が進む中で、これに対応できる革新的な新材料の開発が緊急課題となっています。
ここで要請されるのは、（１）構造が単純で、しかも多くの情報を正確に処理できること、（２）分子レベルで機能し、また、その機能が容易に制御できること、（３）動的な構造を持ち、目的に応じて迅速に諸特性を変化すること、（４）環境汚染の心配がないこと、（５）安価で大量に供給できること、（６）現在のテクノロジーとのマッチングが良いこと などです。この観点で核酸を見た時、その分子構造は全ての要因を満たしています。つまり、核酸は、分子機能材料の素材としてきわめて適しており、無限の可能性を秘めています。今回の「4th Symposium on DNA / RNA / Protein for Nanotechnology」では、遺伝子核酸を化学的手法により修飾・合成・応用することに取り組んでいる世界最先端の研究者の講演を通じてポストゲノムにおける生命
化学、特に核酸研究の新しい潮流について議論することを目的としました。
会告などが諸般の事情により遅れたにもかかわらず、６０人程度の方々にご参加頂き、ミニシンポジウムに相応しい突っ込んだ議論も盛り上がり、期待以上の成果が上がったと世話役一同喜んでおります。御協賛頂き、有り難うございました。

ISBC2003 プレシンポジウムの案内******************
ISBC2003 プレシンポジウム；第４回機能性核酸の創製ミニ国際シンポジウム
開催期日：１２月２日 ９：００〜１５：４０
場

所 ： 兵 庫 県 立 淡 路 夢 舞 台 国 際 会 議 場 （ 〒 656-2301 兵 庫 県 津 名 郡 東 浦 町 夢 舞 台 1 番 地 ・

TEL:0799-74-1020 FAX:0799-74-1021；http://www.yumebutai.org/）
参加費：無料（事前も登録不要です。）
ISBC2003 初日、レセプション開催日に同じ会場である淡路夢舞台国際会議場で DNA / RNA / Protein の
ナノテクノロジーへの応用を視野に入れた最前線の研究を Junghun Suh (Seoul National University), Jik
Chin (University of Toronto), Naoki Sugimoto (Konan University), Peter E. Nielsen (University of
Copenhagen), Makoto Komiyama (University of Tokyo), Yoshio Okahata (Tokyo Institute of Technology)の

生命化学研究レター No. 13 (2004. January) 36

６人の演者からご講演いただきます。ISBC2003 と重複する演者の方も、異なった題目、内容でご講演いただけますので、ISBC2003 参加者もプレシンポジウムに是非ご参加下さい。

4th Symposium on DNA / RNA / Protein for Nanotechnology
Dec. 02, 2003
Conference Hall, Awaji Yumebutai International Conference Center, Hyogo, Japan

09:00 - 09:10

Opening Remark: Makoto Komiyama (University of Tokyo)

Chairman: Makoto Komiyama
09:30 - 10:10

Junghun Suh (Seoul University)"Artificial Proteases with Site Selectivity and Substrate Selectivity".
10:10 – 10:50

Jik. Chin (Seoul University)"Cooperativity Between Metal Coordination and H-bonding:From Anion Recognition to Ester,
Amide and Phosphate Cleavage"
10:50 – 11:00

Coffee Break

Chairman:
11:00 – 11:40

Naoki Sugimoto (Konan University)"Thermodynamics of natural and unnatural nucleic acids"

11:40 – 13:00

Lunch

Chairman: Naoki Sugimoto
13:00 – 13:40

Peter E. Nielsen (University of Copenhagen)“Control of Gene Expression by Peptide Nucleic Acids (PNA)”
Chairman: Naoki Sugimoto
13:40 – 14:20

Makoto Komiyama (University of Tokyo)“Site-Selective Scission of Nucleic Acids - Leading to New Molecular Biology-”
14:20 – 15:20

Yoshio Okahata (Tokyo Institute of Technology)"Gravimetric Kinetics of Enzyme Reactions on a QCM"
15:30 – 15:40

18:00~

Closing Remark: Makoto Komiyama (University of Tokyo)

International Symposium on Bioorganic Chemistry 2003
(ISBC2003) Reception

生命化学研究レター No. 13 (2004. January) 37

Post-ISBC 2003 in Kyushu University(post-international symposium on biomolecular chemistry 2003)
日時：03/12/06(sat)場所：九州大学国際ホール

参加人数：50 名程度
プログラム：(講演者名[所属]／講演タイトル)
Prof. David Fairlie [University of Queensland]／Towards Mimics of Protein Surfaces
Prof. Akio Ojida [Kyushu University]／Novel Artificial Receptors andchemosensors toward phosphorylated
peptide/protein
Prof. Shinya Tsukiji[University of Tokyo]／An Alcohol Dehydrogenase Ribozyme
Prof. Shigeki Sasaki [Kyushu University]／Molecular Design for Gene-Targeting Strategy
Prof. Takahiko Nojima [Kyushu University]／Visualization of DNA duplex region by a new fluorescent peptide
Prof. Young Chang [New York University]／Tagged small molecule approach to facilitated chemical genomics
and proteomics
Prof. Shigeki Kiyonaka [Kyoto University]／Development and application of novel saccharide-based biomaterials
by combinatorial approach
Prof. Peter Seeberger [ETH Zurich]／Use of Synthetic Oligosaccharides in Glycobiology: Carbohydrate
Arrays, Carbohydrate-RNA Interactions and Vaccines生命化学研究レター No. 13 (2004. January) 38

ISBC2003 Post Symposium 報告
大阪府立大学

ニューフロンティア材料研究会
The Society of New Frontier Materials Science and Engineering(Osaka Prefecture University)
平成１５年１２月８日（月）学術交流会館
第１８９回 講演会
主題

Chemical Biology: 生命現象を制御する化学的アプローチ
21 世紀を迎え，「生物」と「化学」の境界領域というように，従来の学問の枠組みを越えた領域
で研究が大きな進展をみせています．特に，この 20 年間に大きく発展した分子生物学は，今や，化学的な物質観なしには研究を進めるのが難しいとまで言われており，生命現象を分子レベルで制御する化学的アプローチが，大きな威力を発揮すると期待されています．また，生物学への化学的アプローチは，プロテオーム，コンビナトリアル・ケミストリー，計算化学などを多用し，それ自身が医薬品の開発に繋がります．今回の講演会では，この新しい分野で世界のトップを走っている
研究者を海外からお招きし最新の話題をお話しいただきます．多くの方々のご参加をお願い申し上げます．

講演会（13:00 〜 18:00）
講演 1.（13:00 〜 14:10）"Designed Polydactyl Zinc Finger Proteins: Building software and hardware for genomes"
Professor Carlos F. Barbas, III(Department of Molecular Biology, The Scripps Research Institute, USA)講演 2.（14:10 〜 15:20）“ファージ・ライブラリーを基軸とした受容体リガンドの分子設計（Directed Evolution of Biofunctional
Molecules in Phage-displayed Combinatorial Libraries）”
藤井 郁雄 （大阪府立大先端科学研究所・教授）
休憩生命化学研究レター No. 13 (2004. January) 39

講演 3.（15:40 〜 16:50）:“New Chemical Genetic Analysis of Cellular Signal Transduction"
Professor Kevan Shokat(Department of Cellular and Molecular Pharmacology, UC San Francisco, USA)

講演会終了後懇親会 （17:00 〜 18:00）

平成１５年１２月８日（月）学術交流会館

２００３年１２月８日（月）に ISBC2003 ポストシンポジウムとして、大阪府立大学ニューフロンティア材料研究会第１８９講演会が開催（開催場所：大阪府立大学学術交流会館）された。 まず、大阪府立大学先端科学研究所の藤井郁雄教授により「ファージライブラリーを機軸とした受容体リガンドの分子設計」と題して、
Chemical Biology に関する基礎的な解説に続いて、ファージライブラリーを用いた GCSF 受容体に親和性を有するペプチドの取得およびそれに続く低分子化合物の設計について発表された。引き続き、 The
Scripps Research Institute の Carlos F. Barbas, III 教授により「Designed Polydactyl Zinc Finger Proteins:
Building software and hardware for genomes」と題して講演が行われた。Barbas 教授らが最近開発した人工zinc finger protein による遺伝子発現の制御についての結果が発表された。最後に、UC San Francisco のKevan Shocat 教授による「New Chemical Genetic Analysis of Cellular Singnal Transduction」と題して、設計された kinase inhibitor と変異 kinase を用いた chemical genetics についての最近の結果が発表された。当日は約１００人の参加者による活発な討論が行われた。

藤井郁雄教授Carlos F. Barbas, III 教授

Kevan Shokat 教授（大阪府立大学先端科学研究所 円谷 健）

生命化学研究レター No. 13 (2004. January) 40

公開セミナー

Professor Tom W. Muir
The Rockefeller University
Selima and Lawrence Ruben Laboratory of Synthetic Protein Chemistry"The Chemical Biology of Protein Splicing"
平成１５年１２月８日（月）午後４時００分
東京大学薬学部第一講義室

菊地 和也（内線２４８５３）
長野 哲雄（内線２４８５０）

公開セミナー

Professor Shao Q. Yao
National University of Singapore
Department of Chemistry & Department of Biological Sciences"Chemical proteomics-An Organic Chemist’s perspectives toward Challenges
in the Post-Genome Era-"
平成１５年１２月８日（月）午後２時００分
東京大学薬学部第一講義室

菊地 和也（内線２４８５３）東京大学大学院薬学系研究科
長野 哲雄（内線２４８５０）東京大学大学院薬学系研究科
三原 久和（０４５−９２４−５７５６）東京工業大学大学院生命理工学研究科

生命化学研究レター No. 13 (2004. January) 41

公開セミナー

Professor David P. Giedroc
Texas A&M University, Department of Biochemistry & Biophysics"Metal Ion Selectivity and Allosteric Switching in Bacterial Metal Sensor Proteins"
平成１５年１２月９日（火）午後３時３０分
東京大学薬学部第一講義室
菊地 和也（内線２４８５３）薬学系研究科
長野 哲雄（内線２４８５０）薬学系研究科
塩谷 光彦（内線２８０６１）理学系研究科化学教室

ＩＳＢＣポストコンファレンス （北里大学理学部セミナー）

平成 15 年 12 月 10 日(水) 午後 4 時 30 分 〜 5 時 30 分
S 号館 3 階 セミナー室２・３（310・311）" Metal Ion Selectivity and Allosteric Switching in Bacterial Metal Sensor Proteins "（バクテリア金属センサー蛋白質における金属イオン選択性とアロステリックスイッチング）

Prof. David P. Giedroc
Department of Biochemistry and Biophysics, Center for Advanced Biomolecular Research, Texas A&M
University, College Station, TX 77843-2128 USA

The SmtB/ArsR family of homodimeric “winged” helix-turn-helix (HTH) proteins represses the expression of
operons associated with metal ion sequestration or efflux in bacteria, allowing these organisms to survive
when challenged with toxic concentrations of heavy metal ions.

Comparative structural and spectroscopic

studies of six SmtB/ArsR proteins reveal that individual members harbor one or two pairs of heavy metalbinding sites, designated α5 and α3N. Recent insight from x-ray crystallographic and NMR experiments on
two related zinc sensors, cyanobacterial SmtB and S. aureus CzrA, on the mode of metal binding and themechanism of metal-induced allosteric switching of low-affinity and high-affinity DNA-binding
conformations will be discussed.

理学部化学科反応機構学講座 大石茂郎・石田 斉 （内線：8159）

生命化学研究レター No. 13 (2004. January) 42

お知らせコーナー

受賞のお知らせ叶 直樹氏（理化学研究所（RIKEN）中央研究所 抗生物質研究室）
受賞名： 第 45 回天然有機化合物討論会奨励賞
Young Investigator Award at the 45th Symmposium on Natural Products Chemistry, 2003
「官能基非依存型低分子アレイの作成と評価」

田中健太郎氏（東京大学大学院理学系研究科化学専攻）
受賞名： 錯体化学会研究奨励賞（2003 年 9 月 25 日）” Efficient Incorporation of Copper Hydroxypiridone Base Pair in DNA”

会員異動のお知らせ
有賀克彦氏 （2004 年 1 月より）独立行政法人 物質・材料研究機構 物質研究所 超分子グループ ディレクター
〒305-0044 茨城県つくば市並木１−１
E-mail: kthk_arg@yahoo.co.jp

菅 裕明氏
東 京 大 学 先 端 科 学 技 術 研 究 セ ン タ ー ケ ミ カ ル バ イ オ テ ク ノ ロ ジ ー ラ ボ (Chemical Biology andBiotechnology Laboratory) 助教授〒153-8904 東京都目黒区駒場４丁目６番１号
Tel&Fax: 03-5452-5495
E-mail: hsuga@rcast.u-tokyo.ac.jp

生命化学研究レター No. 13 (2004. January) 43

平野智也氏（東京大学薬学部薬品代謝化学教室（長野研）博士研究員から 2003 年 7 月より）University of California 博士研究員
c/o Prof. Kevan M. Shokat
Department of Cellular and Molecular Pharmacology,
University of California, San Francisco
600-16th Street, Genentech Hall N514
San Francisco, CA 94107-2280, USA
Tel: +1-415-514-4111
E-mail: th1109jp@yahoo.co.jp

編集後記
あけましておめでとうございます。
昨年１２月に、第１回生命化学国際会議が淡路島で開催されました。研究会にとってエポックメーキングな
出来事であったことは間違いないと思います。大変活気のある会議の中で、皆様はどのような感想をお持ちになられたでしょうか。お聞かせいただければ幸いです。
今回の国際会議の記録のため、特集号として、本号ニュースレターを編集しました。ご参加いただいた
方々にはプロシーディングスと重複がありますが、記録として残すと言う意味でご容赦いただければ幸いです。次号は、国際会議でポスター賞を受賞された方々の寄稿を中心に、来月（2004 年 2 月）、発行予定です。
ご期待ください。
国際会議も終えた１２月のある日、近所を散歩していて、ミノムシがいないことに気付きました。農薬とか環境汚染とか、いろいろ可能性を考えながら歩いていましたが、後で、中国から来た「みのがやどりばえ」というはえに寄生されて、絶滅寸前なのだと知りました。交通機関の発展によって、生態系が壊される環境問題が、人工化学物質による汚染より怖いのかもしれません。皆さんの周りでも、ミノムシがいないか、探してみられてはいかがでしょう。
今年も皆様のご研究の益々のご発展を祈念いたしております。

石田 斉 （北里大学理学部）