Port-a-Patch
The world’s smallest patch clamp set-up

Port-a-Patch

Enjoy electrophysiology
The Port-a-Patch is a turn-key miniaturized patch

Behind its small and compact appearance lies

clamp system enabling the user to rapidly

sophisticated technology, producing high-quality

generate high quality data, regardless ofmeasurements with giga-seals and high successexperience. This makes it ideal as an easy-to-use

rates in all recording configurations. Its strength

tool for learning patch clamp electrophysiology

and flexibility are found in multiple add-ons,

and it can be implemented in many aspects ofproviding the user with a powerful tool for ion

drug discovery and basic ion channel research.

channel research.

Port-a-Patch

Key features at a glance
1

Easy-to-use, even for non-experts

5Temperature control(cooling and heating)2

Voltage and current clamp as standard

6

Compatible with most amplifiers

3

Whole-cell and single channel
recordings

7

Light stimulation (optional)

4

Versatile liquid handling

8

Consumables produced in-housePort-a-Patch

Simple yet versatile
PatchControl is a graphical user interface thatFlexible add-ons for the Port-a-Patch expand thecontrols the parameters needed for cell captureexperimental possibilities.

and sealing. Through innovative communication
with electrophysiology software, PatchControl• Temperature-controlled external perfusion

reads important parameters and moves through• Internal perfusion

pre-defined steps accordingly.

• Light stimulation—SOL• Suction Control Pro• Cell can be captured and sealed automatically• Changes possible at any point by the user
• Compatible with most electrophysiology
programs e.g. PatchMaster, pClamp, WinWCP

PatchControl
The PatchControl software controls cellcapture and sealing. PatchMaster isused for data acquisition and analysis
when the HEKA amplifier is used.

• Microscope slide for simultaneouselectrophysiology and fluorescencemeasurements

Port-a-Patch

Versatile and flexible:
Research highlights
1 µM Glutamate

RT10 µM Glutamate

45 °C

100 µM Glutamate
1 mM Glutamate

0.5 nA

1 nA
200 ms

0 nA

20 ms

Ligand-gated ion channels

Temperature control

The External Perfusion System for the Port-a-Patch

The sophisticated temperature control can

precisely controls application of ligand with fast

be used to activate heat- or cool-activatedexchange time. Exposure time can be minimized

channels such as TRPV4 or TRPM8. Solution isdown to 300 ms. The External Perfusion System canheated or cooled and applied via the bath

be triggered by the electrophysiology software,

solution. Alternatively, recordings can be madethe PatchControl software or manually.

at physiological temperature.

Control

ChR2 H134R (Vh = −100 mV)

2 mM MgCl2
Length of light stimulus
5 ms
25 ms
50 ms

100 pA

50 pA

050 ms

50 ms

Internal Perfusion

Light activation

The Internal Perfusion add-on for the Port-a-PatchThe Port-a-Patch SOL can be used tocan be used to perfuse up to 8 solutions on theactivate light sensitive ion channels such as

inside of the chip. Compounds or ions can bechannelrhodopsin (ChR) or caged compounds.

added to the internal side of the chip to activate

Light intensity and duration can be controlled

or block ion channels.

automatically through the electrophysiology
software or manually.

Port-a-PatchYour research, our passion

Ion channels

Assay development and validation

Ideal for both voltage- and ligand-gated ion

Full flexibility to design assays for a wide variety ofchannels.

cell lines.

Drug Discovery

CiPA validation study

Suitable for all phases of drug discovery in

The Port-a-Patch is delivered fully compliant withpharma and biotech.

the latest CiPA recommended guidelines.

Academic research

Channelopathies

Easy solutions tailored to ensure your next

Sophisticated functional assays for the discovery

scientific breakthroughs.

of ion channel mutations.

CROs

Primary cell/iPSC profiling

Deliver accurate, reproducible and high quality

Low cell consumption for recording primary anddata on time and within budget.

stem cells.

Port-a-Patch

The Port-a-Patch system includes• Port-a-Patch recording station (including Faraday top)
• Port-a-Patch Suction Control, USB-controlled (no house vacuum needed)• EPC-10 USB amplifier (HEKA Electronics), system compatible with other amplifiers• Desktop PC or Notebook with pre-installed PatchControl & electrophysiology software• NPC-1 borosilicate recording chips• Reagent starter kit• On-site installation support• 1 year warranty with further optional comprehensive service plans available

Specifications
Average whole-cell stability~30 min

Successful whole-cell recordings

70 – 90 % (consistent between cell lines)

Throughput

20 – 50 data points per day

Chip resistance2 – 3.5 MΩ (customized resistances available)

Seal resistance>1 GΩ

Series resistance<10 MΩ

Liquid consumption~30 μl/compound

Perfusion time constant~150 ms

Minimum exposure time

250 msInternal perfusion time constant~5 s

Optical stimulation

Yes (optional)

Current clamp

Integrated as standard

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