近畿大学次世代基盤技術研究所報告 Vol. 9（2018）67－70

+3$(&3$'࡟ࡼࡾᚓࡽࢀࡓ⅕㣤⡿ࡢ࢔࣑ࣟ࣌ࢡࢳࣥ㙐㛗ศᕸ࡜㣗࿡ホ౯࡜ࡢ㛵ಀ㧗ὠ ᆅᚿ*1㸪⚟ᮏ ⏤⣖*2㸪⸨⏣ ᫂Ꮚ*1㸪Ώ㑓 ⩏அ*3㸪㔝ᮧ ṇே*4 

Relationship between Distribution of Amylopectin Chain-length in Cooked Rice Analyzed
by HPAEC-PAD and Tasting Evaluation
Kunimoto TAKATSU*1, Yuki FUKUMOTO*2, Akiko FUJITA*1, Yoshiyuki WATANABE*3,and Masato NOMURA*4


The distribution of amylopectin chain-length in cooked rice, whose cultivars wereKoshihikari, Hitomebore, Hinohikari, Yumepirika and Kirara397, was analyzed by highperformance anion exchange chromatography with a pulsed amperometric detector. In addition,
tasting evaluation for cooked rice was carried out by a series of analytical equipment. The
relationships between the chain-length distribution and the tasting value were examined forfive rice cultivars. The relative amount of amylopectin chain in Koshihikari rice grains was thelargest and the stickiness of Koshihikari rice grainswas the highest. It was indicated that thechain-length distribution would affect stickiness and firmness of cooked rice.
Keywords: Amylopectin, Chain-length Distribution, Cooked Rice,High Performance Anion Exchange Chromatography,Pulsed Amperometric Detection, Tasting Evaluation㸬ࡣࡌࡵ࡟ୡ⏺୕኱✐≀ࡢ୍ࡘ࡜ࡉࢀࡿ⡿ࡣ㸪୺࡟࢔ࢪ࢔ᅪࢆ୰ᚰ࡟୺㣗࡜ࡉࢀ㸪ࡑࡢ኱༙ࡀ࢔ࢪ࢔ࢆ୰ᚰ࡜ࡋࡓᅜࠎ࡛సࡽࢀ࡚࠸ࡿ㸬ୡ⏺ࡢ⡿ࡢᖺ㛫⏕⏘㔞ࡣ⣙ 4 ൨ 8000 ୓ࢺࣥ㸦⢭⡿࣮࣋ࢫ㸧࡜࡞ࡗ࡚࠾ࡾ㸪᪥ᮏࡢᖺ㛫⏕⏘㔞ࡣୡ⏺➨ 11 ఩ࡢ 7600 ༓ࢺ࡛ࣥ࠶ࡿ㸦2017 ᖺ࢔࣓ࣜ࢝㎰ົ┬ㄪ࡭㸧㸬᪥ᮏࡢ୺㣗ࡶ⡿࡛࠶ࡿࡀ㸪㣗⏕άࡢኚ໬࡟ࡼ
ࡾ⡿ࡢᾘ㈝ࡢࣆ࣮ࢡࡣ᫛࿴ 37 ᖺ࠿ࡽ୍㈏ࡋ࡚ῶᑡഴྥ

࡟࠶ࡿ㸬᫛࿴ 37 ᖺࡢ୍ேᙜࡓࡾࡢᾘ㈝㔞ࡣ 118 kg ࡛࠶ࡗࡓࡀ㸪ᖹᡂ 28 ᖺᗘ࡟ࡣ 54 kg ࡛༙ศ௨ୗ࡟ࡲ࡛ῶᑡࡋ࡚࠸ࡿ㸬ࡑࢀ࡟క࠸㸪୺㣗⏝⡿ࡢ඲ᅜ࣮࣋ࢫ࡛ࡢ㟂せ㔞ࡣẖᖺ⣙ 8 ୓ࢺࣥࡎࡘࡢῶᑡഴྥ࡟࠶ࡿ㸬ࡇࡢࡼ࠺࡞୰㸪㏆ᖺ㣗࿡࡬ࡢ㛵ᚰࡢ㧗ࡲࡾࢆ⫼ᬒ࡟࠾࠸ࡋ࠸⡿ࢆసࡿࡓࡵ࡟ရ✀ᨵⰋࡀ⾜ࢃࢀ࡚ࡁࡓ㸬୍⯡ⓗ࡟⡿ࡢ㣗࿡ホ౯ࡣ㸪እほ㸪㤶ࡾ㸪࿡㸪⢓ࡾ࠾ࡼࡧ◳ࡉࡢ㡯┠࡛ホ౯ࡉࢀࡿ㸬≉࡟◳ࡉ㸪⢓ࡾ࠾ࡼࡧ⢶໬≉ᛶ࡞࡝ ཎ✏ཷ௜ 2018 ᖺ 4 ᭶ 28 ᪥*1 ᰴᘧ఍♫ࢧࢱࢣ 㣗࿡◊✲ᐊ㸦ࠛ739-8602 ᮾᗈᓥᕷす᮲すᮏ⏫ 2-30㸧
E-mail g-syokumi@satake-japan.co.jp*2 ㏆␥኱Ꮫ ᕤᏛ㒊 ໬Ꮫ⏕࿨ᕤᏛ⛉㸦ࠛ739-2116 ᮾᗈᓥᕷ㧗ᒇ࠺ࡵࡢ㎶ 1 ␒㸧
*3 ㏆␥኱Ꮫ ኱Ꮫ㝔ࢩࢫࢸ࣒ᕤᏛ◊✲⛉ ࢩࢫࢸ࣒ᕤᏛᑓᨷ ᩍᤵ㸪ᕤᏛ㒊 ໬Ꮫ⏕࿨ᕤᏛ⛉ ᩍᤵ㸪ḟୡ௦ᇶ┙ᢏ⾡◊✲ᡤ ᩍᤵ㸦ࠛ739-2116 ᮾᗈᓥᕷ㧗ᒇ࠺ࡵࡢ㎶ 1 ␒㸧E-mail wysyk@hiro.kindai.ac.jp*4 ㏆␥኱Ꮫ ኱Ꮫ㝔ࢩࢫࢸ࣒ᕤᏛ◊✲⛉ ࢩࢫࢸ࣒ᕤᏛᑓᨷ ᩍᤵ㸪ᕤᏛ㒊 ໬Ꮫ⏕࿨ᕤᏛ⛉ ᩍᤵ㸦ࠛ739-2116 ᮾᗈᓥᕷ㧗ᒇ࠺ࡵࡢ㎶ 1 ␒㸧
E-mail nomura@hiro.kindai.ac.jp 㐃⤡ඛ㸸Ώ㑓⩏அ㸦◊✲௦⾲⪅㸧

－ 67 － ࡢ≀⌮໬Ꮫⓗᛶ≧ࡣ㸪㣗࿡ࡢ⥲ྜホ౯ࡢ 70㸣ࢆ༨ࡵ࡚࠸
ࡿ࡜࠸ࢃࢀ࡚࠸ࡿ(1)㸬⡿ࡢ⢓ࡾࡣ⃦⢊࡟⏤᮶ࡋ࡚࠾ࡾ㸪⡿ࡢ඲ᡂศࡢ࠺ࡕ⣙ 70㸣௨ୖࡀ⃦⢊࡛ᵓᡂࡉࢀ࡚࠸ࡿ㸬୍⯡ⓗ࡞࠺ࡿࡕ⡿ࡢ⃦⢊ࡣ㸪┤㙐≧ᵓ㐀ࡢࡓࡵ⢓ᛶ࡬ࡢᐤ୚ࡢᑠࡉ࠸࢔࣑࣮ࣟࢫࡀ⣙ 2 ๭㸪ศᒱᵓ㐀ࢆ᭷ࡍࡿࡓࡵ࡟㧗࠸⢓ᛶ࡟ᐤ୚ࡍࡿ࢔࣑ࣟ࣌ࢡࢳࣥࡀ⣙ 8 ๭࡛ᵓᡂࡉࡇࢀࡽࡢᡂศẚ⋡ࡀ㣗࿡ࢀ࡚࠸ࡿࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿ(2)㸬
࡟ᙳ㡪ࢆ୚࠼࡚࠸ࡿ࡜ࡉࢀࡿ㸬୰࡛ࡶ࢔࣑ࣟ࣌ࢡࢳࣥࡣ⡿⃦⢊ࡢ⣙ 8 ๭ࢆ༨ࡵࡿࡓࡵ㸪ࡑࡢᵓ㐀ࡀഹ࠿࡟ኚ໬ࡍࡿࡔࡅ࡛ࡶ㸪⃦⢊ࡢ≀ᛶࡣ኱ࡁࡃኚ໬ࡋ㸪ࡑࡋ࡚⡿ࡢ⢓ࡾࡸ㌾ࡽ࠿ࡉ࡞࡝࡟኱ࡁ࡞ᙳ㡪ࢆ୚࠼ࡿࡇ࡜࡟࡞ࡿ(3)㸬
 ࡇࢀࡲ࡛⡿ࡢ࢔࣑ࣟ࣌ࢡࢳࣥ◊✲ࡣ㸪⢭ⓑ⡿ࢆ⏝࠸ࡓ౛ࡀ୺࡛࠶ࡾ㸪⅕㣤ᚋࡢ⡿ࡢ࢔࣑ࣟ࣌ࢡࢳࣥ࡟ࡘ࠸࡚ࡢ◊✲஦౛ࡣከࡃ࡞࠸㸬ࡋ࠿ࡋ㸪⡿ࡢ㣗࿡ホ౯ࡣ⅕㣤⡿ࢆᑐ㇟࡟⾜ࢃࢀࡿࡶࡢ࡛࠶ࡿࡓࡵ㸪⅕㣤⡿୰ࡢ࢔࣑ࣟ࣌ࢡࢳࣥࡢศᏊᵓ㐀࡟㛵ࡍࡿ▱ぢࡀồࡵࡽࢀ࡚࠸ࡿ㸬ࡑࡇ࡛ᮏ◊✲࡛ࡣ㸪5 ရ✀ࡢ᪂⡿ࢆ⅕㣤ᚋ㸪ࣃࣝࢫࢻ࢔ࣥ࣌ࣟ
࣓ࢺ᳨࣮ࣜฟჾࢆ⏝࠸ࡓ㝜࢖࢜ࣥ஺᥮ࢡ࣐ࣟࢺࢢࣛࣇ࢕࣮(HPAEC-PAD)࡟࡚㸪⡿⢏࡟ྵࡲࢀࡿ࢔࣑ࣟ࣌ࢡࢳࣥࡢ
ࡉࡽ࡟⡿ࡢ㣗࿡ィ ᶵჾࢆ⏝࠸࡚㙐㛗ศᕸࢆ ᐃࡋࡓ(4)㸬
ᐃ㔞ⓗ࡟ホ౯ࡉࢀࡓ㣗࿡್࡜࢔࣑ࣟ࣌ࢡࢳࣥ㙐㛗ศᕸ࡜ࡢ㛵ಀᛶ࡟ࡘ࠸᳨࡚ウࡋࡓ㸬㸬ᐇ㦂᪉ἲ
 ᮦᩱ࠾ࡼࡧヨ⸆ ᖹᡂ 29 ᖺ࡟཰✭ࡉࢀࡓ໭ᾏ㐨⏘ࡁࡽࡽ 397㸪໭ᾏ㐨⏘ࡺࡵࡨࡾ࠿㸪ᐑᇛ┴⏘ࡦ࡜ࡵࡰࢀ㸪᪂₲┴⏘ࢥࢩࣄ࢝ࣜ
࠾ࡼࡧᗈᓥ┴⏘ࣄࣀࣄ࢝ࣜࡢ 5 ရ✀ࢆ⡿ヨᩱ࡜ࡋࡓ㸬࡞࠾㸪⡿ヨᩱࡣࢸࢫࢺ⢭⡿ᶵ㸦MC-250 ᆺ㸪ओࢧࢱࢣ〇㸧࡟㐺⏝ࡋ࡚⢭⡿ࡉࢀࡓ㸬≉⣭࢚ࢱࣀ࣮ࣝࡣ㛵ᮾ໬Ꮫओ࠿ࡽ㸪≉⣭ 1-ࣈࢱࣀ࣮ࣝࡣ࢟ࢩࢲ໬Ꮫओ࠿ࡽ㸪≉⣭ࢳ࣮ࣔࣝࡣᮾி໬ᡂᕤᴗओ࠿ࡽ㸪≉⣭࢔ࢭࢺࣥࡣ⡿ᒣ⸆ရᕤᴗओ࠿ࡽ㸪࢖ࢯ࢔࣑࣮ࣛࢮࡣࢩࢢ࣐-࢔ࣝࢻࣜࢵࢳ࠿ࡽ㸪≉⣭㓑㓟㸪≉⣭㓑㓟ࢼࢺ࣒ࣜ࢘㸪≉⣭ࢪ࢚ࢳ࢚࣮ࣝࢸࣝ㸪≉⣭ࢪ࣓ࢳࣝࢫࣝ࣍࢟ࢩࢻ(DMSO)࠾ࡼࡧ≉⣭Ỉ㓟໬ࢼࢺ࣒ࣜ࢘ࡣ࿴ග⣧⸆ᕤᴗओ࠿ࡽ㸪ࡑࢀࡒࢀ㉎ධࡋ౑⏝ࡋࡓ㸬 ⡿ࡢ⅕㣤
⡿ヨᩱ 450 g ࡟Ỉ㐨Ỉࢆ୍ᐃࡢ㏿ᗘ࡛ὶỈࡉࡏ࡞ࡀࡽ
Ὑ⡿ჾ㸦DK-SA26㸪㇟༳࣐࣮࣍ࣅࣥओ㸧ࢆ⏝࠸࡚ 10 ᅇ࠿ࡁΰࡐ㸪Ỉࢆᤞ࡚ࡿ᧯సࢆ 5 ᅇ⧞ࡾ㏉ࡋࡓ㸬ࡑࡢᚋ㸪Ỉ㐨Ỉ 630 mL ࢆຍ࠼㸪⅕㣤ჾ㸦SR-HS 104㸪ࣃࢼࢯࢽࢵࢡओ㸧࡟࡚⅕㣤ࡋࡓ㸬ຍỈ㔞ࡣ㉁㔞ẚ࡛⡿ࡢ 1.40 ಸ࡜ࡋ㸪ᾐₕ᫬㛫࡜⵨ࡽࡋ᫬㛫ࢆࡑࢀࡒࢀ 30 ศ㛫࡜ࡋࡓ㸬 ⡿⃦⢊ࡢㄪ〇
⅕㣤ᚋ㸪30 ศ㛫⵨ࡽࡋࡓ⡿ࢆ࣮࣌ࣃ࣮ࢱ࢜ࣝࡢୖ࡟⨨ࡁ 30 ศ㛫✵Ẽ㞺ᅖẼୗ࡛㟼⨨ࡉࡏࡓ㸬ࡑࡢᚋ㸪5 g ࡢ⅕㣤⡿ࢆࣅ࣮࣮࢝࡟㔞ࡾྲྀࡗࡓ㸬0.2% (w/w) Ỉ㓟໬ࢼࢺ࣒ࣜ࢘Ỉ⁐ᾮࢆ 150 mL ຍ࠼㸪ࣛ࣎ࢫࢱ࣮࣮ࣛࢆ⏝࠸࡚ 3᫬㛫᧠ᢾࢆ⾜࠸㸪21 ᫬㛫㟼⨨ࡋࡓ㸬ୖ⃈ࡳᾮࢆ㝖ཤࡋࡓᚋ࡟ከ㔞ࡢ⵨␃Ỉࢆຍ࠼࡚᧠ᢾࡍࡿ᧯సࢆ㸪⅕㣤⡿⁐ᾮࡢ pH ࡀ 7.0 ࡟࡞ࡿࡲ࡛⧞ࡾ㏉ࡋࡓ㸬6,500 rpm ࡛ 5 ศ㛫ࡢ㐲ᚰศ㞳ࢆ⾜࠸㸪ỿẊ≀ࢆ෾⤖஝⇱ࡋࡓ㸬 ࢔࣑ࣟ࣌ࢡࢳࣥࡢㄪ〇ୖグ 2.3 ࡛ᚓࡽࢀࡓ஝⇱≀ 0.5 g ࡜ DMSO 15 mL ࢆࢼࢫࣇࣛࢫࢥ୰࡛ΰྜࡋ㸪ࢫࢱ࣮࣮࡛ࣛ⣙ 24 ᫬㛫ࡺࡗࡃࡾ᧠ᢾࡋ࡚ヨᩱࡢ⢏ᵓ㐀ࢆ◚ቯࡉࡏ࡞ࡀࡽศᩓ࠾ࡼࡧ⁐ゎࡉࡏࡓ㸬ࡇࡢ⁐ᾮ࡟ 1-ࣈࢱࣀ࣮ࣝ 25 mL ࢆ⣲᪩ࡃὀࡂ24 ᫬㛫㟼⨨ࡋࡓ㸬⏕ࡌࡓỿẊ≀ࢆࡉࡽ࡟ 1-ࣈࢱࣀ࣮࡛ࣝ
ᩘᅇὙίࡋ࡚ DMSO ࢆ㝖࠸ࡓ㸬ỿẊ≀࡟⵨␃Ỉ 50 mLࢆຍ࠼㸪࢜࢖ࣝࣂࢫ୰࡛ 100Υ࡟ಖࡕ࡞ࡀࡽ❅⣲Ẽὶୗ
࡛ 2 ᫬㛫ຍ⇕㑏ὶࢆ⾜ࡗࡓ㸬ࡑࡢᚋ㸪60Υ௨ୗࡲ࡛෭༷
ࡉࡏࢳ࣮ࣔࣝ 50 mg ࢆຍ࠼࡚ᆒ୍࡟ΰࡐ㸪ᐊ ୰࡟ 3 ᪥㛫㟼⨨ࡉࡏ࢔࣑࣮ࣟࢫ-ࢳ࣮ࣔࣝ」ྜయࢆᚓࡓ㸬㐲ᚰศ㞳
㸦6,500 rpm㸪5 ศ㛫㸧ࢆ⾜࠸㸪ᚓࡽࢀࡓୖ⃈ࡳᾮࢆ࢔࣑
ࣟ࣌ࢡࢳࣥ⏬ศ࡜ࡋࡓ㸬෾⤖஝⇱࡟ࡼࡾࡇࡢ⏬ศࡢ⣙ 1/3㔞࡟⃰⦰ࡋ㸪
2 ಸ㔞ࡢࢪ࢚ࢳ࢚࣮ࣝࢸࣝࢆຍ࠼Ὑίࡋࡓ㸬Ỉᵴ㒊ศࢆࣇ࢓ࣝࢥࣥࢳ࣮ࣗࣈ࡟᥇ྲྀᚋ㸪ࡑࢀ࡟ 2㹼3 ಸ㔞ࡢ࢚ࢱࣀ࣮ࣝࢆຍ࠼࡚෌ࡧ㐲ᚰศ㞳㸦6,500 rpm㸪5 ศ㛫㸧ࡋࡓ㸬ᚓࡽࢀࡓỿẊ≀࡟ࡘ࠸࡚㸪෌ᗘ࢚ࢱࣀ࣮ࣝࢆ
⏝࠸࡚Ὑίࢆ⾜࠸㸪࢔ࢭࢺ࡛ࣥ⬺Ỉࡉࡏ࡚࢔࣑ࣟ࣌ࢡࢳࣥࢆᚓࡓ㸬࢔࣑ࣟ࣌ࢡࢳࣥ 100 mg ࡟⵨␃Ỉ 20 mL ຍ࠼㸪70Υࡢᜏ Ỉᵴ࡟࢖࣮ࣥ࢟ࣗ࣋ࢺࡋ࡚ຍ⇕⢶໬ࡉࡏࡓ㸬ࡑࡢᚋ㸪㓑㓟⦆⾪ᾮ(pH 4.5)20 mL ࠾ࡼࡧ࢖ࢯ࢔࣑࣮ࣛࢮ(755 U)ࢆຍ࠼㸪45Υ࡛ 15 ᫬㛫ࡢ㓝⣲཯ᛂ⾜ࡗࡓ㸬཯ᛂᚋ㸪100Υࡢຍ⇕࡟ࡼࡾ㓝⣲ࢆኻάࡉࡏࡓ㸬෾⤖஝⇱ᚋ㸪200 mg ࢆ࢚ࢵ࣌ࣥࢳ࣮ࣗࣈ࡟㔞ࡾྲྀࡾ㸪1 mL ⵨␃Ỉࢆຍ࠼ࡓ㸬100Υ࡛ 10 ศ㛫ࡋ࡚⢶໬ࡋࡓᚋ㸪࣓ࣥࣈࣞࣥࣇ࢕ࣝࢱ࣮࡛ࢁ㐣ࡋ㸪ࡇࢀࢆศᯒヨᩱ࡜ࡋࡓ㸬 +3$(&3$' ࢆ⏝࠸ࡓศᯒ㔠㟁ᴟࢆ⏝࠸ࡓࣃࣝࢻ࢔࣓ࣥ࣌ࣟࢺ᳨࣮ࣜฟჾࢆ⏝࠸ࡓ㝜࢖࢜ࣥ஺᥮ࢡ࣐ࣟࢺࢢࣛࣇ࢕࣮㸦ISC-5000㸪ࢧ࣮ࣔࣇ࢕ࢩ࣮ࣕࢧ࢖࢚ࣥࢸ࢕ࢵࢡࢫओ㸧࡟࡚࢔࣑ࣟ࣌ࢡࢳࣥ㙐㛗ศᕸࢆ ᐃࡋࡓ㸬࣒࢝ࣛ ᗘࡣᖖ ࡜ࡋ㸪㏦ᾮὶ㔞ࡣ 1.0 mL/min ࡜ࡋࡓ㸬ࢢࣛࢪ࢚ࣥࢺ⁐ฟ᧯స࡛ࡣ㸪⁐㞳ᾮ A ࡟ 100 mM Ỉ㓟໬ࢼࢺ࣒ࣜ࢘Ỉ⁐ᾮ㸪⁐㞳ᾮ B
࡟100 mMỈ㓟໬ࢼࢺ࣒ࣜ࢘/500 mM㓑㓟ࢼࢺ࣒ࣜ࢘Ỉ⁐ᾮ࠾ࡼࡧ⁐㞳ᾮ C ࡟ࡣ㉸⣧Ỉࢆ⏝࠸ࡓ㸬⁐ฟ㛤ጞ᫬ࡣ
A㸸B㸸C = 60㸸40㸸0 (v/v/v)㸪㛤ጞ 2 ศᚋ࡟ A㸸B㸸C= 50㸸50㸸0 (v/v/v)㸪40 ศᚋ࡟ A㸸B㸸C = 20㸸80㸸
0 (v/v/v)࡜࡞ࡿࡼ࠺࡟ࢢࣛࢪ࢚ࣥࢺ⁐ฟࢆᐇ᪋ࡋࡓ㸬－ 68 － 㣗࿡ホ౯⡿ࡢ㣗࿡ホ౯࡟ࡣ㸪⅕㣤㣗࿡ィ㸦STA1B㸪ࢧࢱࢣओ㸧㸪◳ࡉ࣭⢓ࡾィ㸦RHS1A㸪ࢧࢱࢣओ㸧࠾ࡼࡧࢩࣥࢭࣥࢧ
㸦RFDM1A㸪ࢧࢱࢣओ㸧࡛ᵓᡂࡉࢀࡿ㣗࿡㚷ᐃᅋࢆ⏝࠸ࡓ㸬⅕㣤㣗࿡ィ࡟ࡼࡿ ᐃ࡛ࡣ㸪⅕㣤ᚋࡢ⡿⢏ࢆ 20 ศ㛫
㏦㢼ᶵ࡟࡚㝆 ࡋࡓᚋ㸪ᐊ ࡟ 100 ศ㛫㟼⨨ࡋࡓࡶࡢࢆヨᩱ࡜ࡋࡓ㸬 8.0 g ࡢヨᩱࢆࢫࢸࣥࣞࢫ〇ࣜࣥࢢ࡟ワࡵ㸪ᐃ⏝ࢭࣝෆ࡛ ᐃࢆ⾜ࡗࡓ㸬 ᐃᅇᩘࡣ㸪1 ヨᩱ࡟ࡘࡁ⾲㠃 1 ᅇ࡜⿬㠃 1 ᅇࡢィ 2 ᅇ࡜ࡋ㸪ࡇࡢ᧯సࢆ 3 ᅇ⧞ࡾ㏉ࡋࡓ㸬◳ࡉ࣭⢓ࡾィࢆ⏝࠸ࡓ ᐃ࡟ࡣ㸪⅕㣤㣗࿡ィ࡛⏝࠸ࡓヨᩱࢆ⥆ࡅ࡚౑⏝ࡋࡓ㸬 ᐃᅇᩘࡣ 1 ヨᩱ࡟ࡘ
ࡁ 1 ᅇ⾜࠸㸪ࡇࡢ᧯సࢆ 5 ᅇ⧞ࡾ㏉ࡋࡓ㸬ࢩࣥࢭࣥࢧ࡛ࡢ ᐃ࡟࠾࠸࡚ࡣ㸪⢭ⓑ⡿ 2.0 g ࡟ RFDM ⏝ฎ⌮ヨ⸆ࢆ
10.0 g ຍ࠼࡚ 1 ศ㛫࢖࣮ࣥ࢟ࣗ࣋ࢺࡋࡓ㸬ࡑࡢᚋ㸪㐲ᚰศ㞳㸦6,200 rpm㸪1 ศ㛫㸧ࢆ⾜࠸㸪ୖ⃈ࡳᾮࢆヨᩱ࡜ࡋ㩭ᗘุᐃࢆ⾜ࡗࡓ㸬 ᐃᅇᩘࡣ 1 ヨᩱ࡟ࡘࡁ 1 ᅇ⾜ࡗࡓ㸬௨ୖࡢ ᐃ࡟ࡼࡾᚓࡽࢀࡓእほ㸪◳ࡉ㸪⢓ࡾ࠾ࡼࡧ㩭ᗘࡢホ౯್ࢆ 10 Ⅼ‶Ⅼ࡜ࡋ㸪⥲ྜホ౯࡛࠶ࡿ㣗࿡㚷ᐃ್ࡀ

100 Ⅼ‶Ⅼ࡛♧ࡉࢀࡓ㸬㸬⤖ᯝ࠾ࡼࡧ⪃ᐹ ࢔࣑ࣟ࣌ࢡࢳࣥ㙐㛗ศᕸ
5 ရ✀ࡢ⅕㣤⡿࠿ࡽᚓࡽࢀࡓ࢔࣑ࣟ࣌ࢡࢳࣥࡢ㙐㛗ศ
ᕸࢆᅗ 1 ࡟♧ࡍ㸬HPAEC-PAD ࢆ⏝࠸࡚භ⢾࡛࠶ࡿ࣐ࣝ
ࢺ࣊࢟ࢧ࣮࢜ࢫ 1 ppm ࢆᇶ‽࡜ࡋࡓ᳨ฟ್ࡢẚࢆ⦪㍈࡟
ྲྀࡾ㸪ᶓ㍈࡟ࡣ㔜ྜᗘ 6 ௨ୖࡢ⢾㙐ࡀ㡰࡟♧ࡉࢀࡓ㸬࠸ࡎࢀࡢရ✀࡟࠾࠸࡚ࡶ㸪㔜ྜᗘࡀቑ࠼ࡿ࡟ࡘࢀ࡚⢾㙐ࡢ᳨ฟ್ࡶቑຍࡋ㸪㔜ྜᗘ 9 ࠿ࡽ 12 ࡢ⠊ᅖ᳨࡛ฟ್ࡀࣆ࣮
ࢡࢆ♧ࡋࡓ㸬ࡑࢀ௨ୖࡢ㔜ྜᗘ࡛ࡣ㸪ᚎࠎ࡟᳨ฟ್ࡀῶ
ᑡࡋ࡚࠸ࡗࡓ㸬᪂₲┴⏘ࢥࢩࣄ࢝ࣜࡣ௚ࡢ 4 ရ✀࡟ẚ࡭㸪᭷ព࡟⢾㙐ᩘࡀከ࠸ࡇ࡜ࡀ♧ࡉࢀࡓ㸬ᚓࡽࢀࡿ࢔࣑ࣟ࣌
ࢡࢳࣥ㔞ࡢ㐪࠸࡟ࡼࡿࡇ࡜ࡀ⪃࠼ࡽࢀࡿࡀ㸪┦ᑐⓗ࡞㙐㛗ศᕸࡀ௚࡜኱ࡁࡃ␗࡞ࡿࡇ࡜ࡀࢃ࠿ࡗࡓ㸬ྛ㔜ྜᗘ࡟࠾ࡅࡿ⢾㙐ᩘࡣ㸪ᴫࡡࢥࢩࣄ࢝ࣜ㸪ࡦ࡜ࡵࡰࢀ㸪ࣄࣀࣄ࢝ࣜ㸪ࡺࡵࡨࡾ࠿㸪ࡁࡽࡽ 397 ࡢ㡰࡛࠶ࡗࡓ㸬6䝁䝅䝠䜹䝸᳨ฟ䛾ẚ㻔n=5ᖹᆒ䠅

5䝠䝜䝠䜹䝸
䜂䛸䜑䜌䜜

4䜖䜑䜄䜚䛛
䛝䜙䜙3973210678

9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28㔜ྜᗘᅗ 1㸬࢔࣑ࣟ࣌ࢡࢳࣥ㙐㛗ศᕸᚓࡽࢀࡓ࢔࣑ࣟ࣌ࢡࢳࣥࡢ㙐㛗ศᕸࢆ㔜ྜᗘ 6㹼12㸪13
㹼20 ࠾ࡼࡧ 21㹼28 ࡢ 3 ༊ศ࡟ศࡅ㸪ࡑࢀࡒࢀࡢ༊㛫࡛ࡢ┦ᑐⓗ⢾㙐ᩘࡢ࿴ࢆ⟬ฟࡋࡓ⤖ᯝࢆ⾲ 1 ࡟♧ࡍ㸬࠸ࡎࢀࡢရ✀ࡶ㔜ྜᗘࡀᑠࡉ࠸ 6㹼12 ࡢ༊㛫ࡀ᭱ࡶ኱ࡁ࡞⢾㙐ᩘࢆ♧ࡋࡓ㸬඲࡚ࡢ༊㛫࡟࠾࠸࡚ࢥࢩࣄ࢝ࣜࡢ⢾㙐㔞ࡀከ࠿ࡗࡓࡀ㸪㔜ྜᗘࡀ኱ࡁࡃ࡞ࡿ࡯࡝௚ࡢရ✀࡜ࡢᕪࡀᗈࡀࡾ㸪≉࡟㔜ྜᗘ 21㹼28 ࡢ༊㛫࡛ࡣከရ✀࡜ࡢ኱ࡁ࡞ศᕸࡢ㐪࠸ࢆ♧ࡋࡓ㸬ࡲࡓ㸪㔜ྜᗘ 6㹼12 ࡟࠾࠸࡚ࡣ㸪ࡦ࡜ࡵࡰࢀࡢ⢾㙐ᩘࡀከࡃ㸪ࢥࢩࣄ࢝ࣜ࡟㏆࠸್ࢆ♧ࡋࡓ㸬ࣄࣀࣄ࢝ࣜࡣ౑⏝ࡋࡓ 5 ရ✀ࡢ୰࡛ࡣ୰㛫ⓗ࡞ᩘ್ࢆ♧ࡋ㸪ࡺࡵࡨࡾ࠿࠾ࡼࡧࡁࡽࡽ 397 ࡣ㸪඲༊㛫࡟࠾࠸࡚⢾㙐ᩘࡀᑡ࡞࠿ࡗࡓ㸬－ 69 －

⾲ 1㸬࢔࣑ࣟ࣌ࢡࢳࣥ㙐㛗ࡢ┦ᑐ㔞ࡢẚ㍑
䝁䝅䝠䜹䝸䝠䝜䝠䜹䝸䜂䛸䜑䜌䜜 䜖䜑䜄䜚䛛䛝䜙䜙397

6䡚12

23.7

20.4

22.9

19

18.3

13䡚20

22.4

14.2

14.8

13.2

11.7

21䡚28

10.4

3.9

3.6

3.5

2.7

0.85䝁䝅䝠䜹䝸 㣗࿡ホ౯್࡜ࡢ㛵ಀ
ୖ㏙ࡉࢀࡓ⡿ရ✀㛫ࡢ࢔࣑ࣟ࣌ࢡࢳࣥ⢾㙐ศᕸࡢ┦㐪ࡀ㸪⅕㣤⡿ࡢ㣗࿡࡟࡝ࡢࡼ࠺࡟ᙳ㡪ࡍࡿ࠿ࢆ᳨ドࡋࡓ㸬⥲ྜⓗ࡞ホ౯್࡜ࡋ࡚ࡢ㣗࿡㚷ᐃ್ࢆᅗ 2 ࡟♧ࡍ㸬㣗࿡್ࡢ㧗ࡉࡣ㸪ࢥࢩࣄ࢝ࣜ㸪ࡦ࡜ࡵࡰࢀ㸪ࡺࡵࡨࡾ࠿㸪ࣄࣀࣄ࢝ࣜ㸪ࡁࡽࡽ 397 ࡢ㡰࡛࠶ࡾ㸪≉࡟ࡁࡽࡽ 397 ࡢ್ࡢపࡉࡀ㢧ⴭ࡛࠶ࡗࡓ㸬㣗࿡㚷ᐃ್ࡢ㡰఩ࡣ㸪࢔࣑ࣟ࣌ࢡࢳࣥ⢾㙐㔞ࡢࡑࢀ࡟ఝࡓഴྥࢆ♧ࡋࡓࡀ㸪ୖ఩ࡢࢥࢩࣄ࢝ࣜ࡜ࡦ࡜ࡵࡰࢀࡢ㛫ࡢ㣗࿡㚷ᐃ್ࡢᕪࡣ኱ࡁࡃ࡞࠿ࡗࡓ㸬92㣗࿡㚷ᐃ್

88
84
807672䝁䝅䝠䜹䝸䝠䝜䝠䜹䝸 䜂䛸䜑䜌䜜 䜖䜑䜄䜚䛛 䛝䜙䜙397

ᅗ 2㸬5 ရ✀ࡢ⅕㣤⡿ࡢ㣗࿡㚷ᐃ್ࡢẚ㍑
ḟ࡟㸪⅕㣤⡿ࡢ㣗࿡ホ౯㡯┠ࡢ୰࡛ࡶ㸪≉࡟࢔࣑ࣟ࣌
ࢡࢳࣥᵓ㐀࡜㛵ಀࡀ῝࠸࡜⪃࠼ࡽࢀࡿ⢓ࡾ࡜◳ࡉ࡜࠸ࡗࡓຊᏛ≉ᛶ್ࡢရ✀㛫ẚ㍑࡟ࡘ࠸᳨࡚ウࡍࡿ㸬5 ရ✀ࡢ⅕㣤⡿ࡢ⢓ࡾ್࡜◳ࡉ್࡜ࡢ㛵ಀࢆᅗ 3 ࡟♧ࡍ㸬ࢥࢩࣄ࢝ࣜࡢ⢓ࡾ್ࡀ㸪௚࡟ẚ࡭ⴭࡋࡃ㧗࠸ࡇ࡜ࡀ♧ࡉࢀࡓ㸬୍᪉࡛㸪ࡁࡽࡽ 397 ࡢ⢓ࡾ್ࡣపࡃ㸪㏫࡟◳ࡉ್ࡣ᭱ࡶ㧗࠸್ࢆ♧ࡋࡓ㸬ヨ㦂࡟⏝࠸ࡓ 5 ရ✀࡟ࡘ࠸࡚㸪ᅗ 1 ࠾ࡼࡧ⾲ 1 ࡟♧ࡉࢀࡓ⢾㙐ᩘ࡜ຊᏛ≉ᛶ್࡜ࡢ㛫࡟᫂☜࡞ഴྥࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࡀ㸪඲యⓗ࡟⢾㙐ᩘࡀከࡃ⢓ࡾ್ࡢ㧗࠿ࡗࡓࢥࢩࣄ࢝ࣜ࡜㸪ẚ㍑ⓗ⢾㙐ᩘࡀᑡ࡞ࡃ㸪≉࡟㔜ྜᗘ 13 ௨ୖࡢ⢾㙐ᩘࡀᑡ࡞࠸௚ࡢ 4 ရ✀࡜ࡣ㸪␗࡞ࡿຊᏛ≉ᛶࣉࣟࣇ࢕ࣝࢆ♧ࡋࡓ㸬ࡇࢀࡽࡢ⤖ᯝࡣ㸪⅕㣤
⡿୰ࡢ࢔࣑ࣟ࣌ࢡࢳࣥ㔞ࡸࡑࡢ⢾㙐㔞ࡀ㸪ᮏ ᐃ࡟࠾ࡅ
ࡿ⅕㣤⡿ࡢຊᏛ≉ᛶ್࡟ᐤ୚ࡍࡿࡇ࡜ࢆ♧ࡋ࡚࠸ࡿ㸬࢔࣑ࣟ࣌ࢡࢳࣥࡢ⢾㙐ศᕸ࡜ᮏ ᐃ࡟࠾ࡅࡿ㣗࿡್࡜ࡢ㛫࡟᫂☜࡞㛵ಀᛶࡀぢฟࡏ࡞࠿ࡗࡓཎᅉ࡜ࡋ࡚㸪࢔࣑࣮ࣟࢫ㔞ࡸ㸪ࡼࡾ㔜ྜᗘࡢ㧗࠸㉸㛗㙐ࡢ⢾㙐ᩘ࡞࡝ࡢᙳ㡪ࡀ⢓䜚 䠷kgf䠹㔜ྜᗘ⪃࠼ࡽࢀࡿ㸬ࡲࡓ㸪⅕㣤⡿ࡸࡑࡢ࢔࣑ࣟ࣌ࢡࢳࣥࡢ≀ᛶホ౯࡟࠾࠸࡚ࡶ㸪⢶໬⢓ᗘࡸ⢶໬⇕㔞࡞࡝ᵝࠎ࡞ᣦᶆࡀ
᥇⏝ࡉࢀ࡚࠸ࡿࡓࡵ㸪ホ౯ᣦᶆࡢ㛵ಀᛶ࡟ࡘ࠸࡚ࡶᗈࡃ᳨ウࡍࡿᚲせࡀ࠶ࡿ࡜⪃࠼ࡽࢀࡿ㸬

0.75
0.65
䜖䜑䜄䜚䛛

0.55

䜂䛸䜑䜌䜜
䛝䜙䜙397

䝠䝜䝠䜹䝸

0.45
3.70

3.90

4.10

4.30

4.50

4.70

4.90

◳䛥 䠷kgf䠹

ᅗ 3㸬⅕㣤⡿ࡢ⢓ࡾ࡜◳ࡉࡢ㛵ಀ㸬ࡲ࡜ࡵ
⅕㣤⡿୰ࡢ࢔࣑ࣟ࣌ࢡࢳࣥ㙐㛗ศᕸࡀ⅕㣤⡿⢏ࡢຊᏛ≉ᛶ್࡟ᐤ୚ࡍࡿࡇ࡜ࡀ♧ࡉࢀࡓ㸬ࡋ࠿ࡋ࡞ࡀࡽ㸪ᮏᐃ࡛ᚓࡽࢀࡓ⢾㙐㔞ศᕸࡔࡅ࡛ࡣ㸪⅕㣤⡿ࡢ㣗࿡࡟ᐤ୚ࡍࡿᅉᏊ࡜ࡋ࡚༑ศ࡛ࡣ࡞࠸ࡇ࡜ࡀ☜ㄆࡉࢀࡓ㸬௒ᚋࡣ㸪࢔࣑ࣟ࣌ࢡࢳࣥ㸭࢔࣑࣮ࣟࢫ㔞ẚࡸ㸪ࡑࢀࡽࡢ㙐㛗ศᕸ࡞࡝ࡢᅉᏊࢆຍ࠼㸪⅕㣤⡿୰ࡢ⃦⢊ᵓ㐀࡜㣗࿡࡜ࡢ㛵ಀ࡟ࡘ࠸᳨࡚ウࡋ࡚࠸ࡃࡇ࡜ࡀồࡵࡽࢀࡿ㸬ࡉࡽ࡟㸪㣗࿡ホ౯࡟࠾࠸࡚ࡣᐁ⬟ヨ㦂ࢆᑟධࡍࡿࡇ࡜ࡀᮃࡲࡋ࠸ࡔࢁ࠺㸬ཧ⪃ᩥ⊩(1) ㇂㐩㞝ࡽ㸪ᰤ㣴࡜㣗⣊㸪22 ᕳ (1969)㸪pp.452-461(2) Uda Y., et al., Recent Advances in Ginseng andGlycosides Research, (2013), pp.137-154(3) Inouchi N., J. Appl. Glycosci., 57 (2010), pp.13-23(4) ᶓ㔝୍Ṍࡽ㸪⨾࿡ᢏ⾡Ꮫ఍ㄅ㸪15 ᕳ 2 ྕ (2016)㸪
pp.15-20－ 70 －