PRODUCT CATALOGUEOva Cryo Kit
Ova Thawing Kit- Ovarian Tissue Vitrification -Quality Resultsfor Lifeʕ Media and devices for ovarian tissue vitrification and thawing.
ʕ Square Measure is a template that helps to prepare ovary into dimensions of 10 x 10 x 1mm.
ʕ Cryodevices are available in both closed and open system.
ʕ Ova Rinse is medium for washing and keeping ovary to prevent it from drying before vitrification
ɻprocedure. Ova Culture is recommended for extended culture after thawing.
Collaborative Development
Prof. Nao SUZUKI, M.D. ,Ph.D., Obstetrics and Gynecology, St. Marianna University School of Medicine
Yoshiharu MORIMOTO, MD., Ph.D., CEO, HORAC GRAND FRONT OSAKA CLINIC

REF

CodeProduct Name

Details

82212

VT301S

2YD&U\R.LW7\SH0&U\RP/ɼ&U\RP/ɼ&U\RP/82222

VT302S

2YD7KDZLQJ.LW7\SH07KDZP/ɼ7KDZP/ɼ7KDZP/

82215

OVR-100

2YD5LQVH

P/

82216

OVCL-100

2YD&XOWXUHP/82217

OVCM-100

2YD&XOWXUHZLWK+(3(6P/81212

6TXDUH0HDVXUH

6TXDUH0HDVXUH &XWWHU3ODWH

5pcs/pk81213

ODT×102YD&U\R'HYLFH7\SH02SHQ6\VWHP

10pcs/pk81214

OCS102YD&U\R6KHHW&ORVHG6\VWHP 

10pcs/pk
Extra pouch for Cryo Device×2
Cane for Ova Cryo SheetʁKOCC ×181215

KOCC102YD&U\R6KHHW&DQH

10pcs/pk

COMPONENTSQUALITY CONTROL

VT301S / VT302S

VT301S / VT302S

HEPES within Basic culture medium /pH7.2-7.6 / Osmolality / Endotoxin <0.25EU/mL / Sterility Test

Ethylene glycol / Gentamicin /Storage: 2-8ˈ

Human serum albumin / Sucrose

Shelf Life: 12 months

OVR-100 / OVCL-100 / OVCM-100

OVR-100 / OVCL-100 / OVCM-100

Calcium chloride / Gentamicin / Glucose /pH7.2-7.6 / Osmolarity 270-295mOsm/L / Endotoxin <0.25EU/mL /Human serum albumin / Magnesium sulfate /Sterility Test / Mouse embryo assay 侔80ˍ

Potassium chloride / Potassium phosphate /Storage: 2-8ˈSodium bicarbonate / Sodium chloride /Shelf Life: OVR-100 / OVCM-100: 8 months

Sodium lactate / Sodium pyruvateɻɻɻɻɻOVCL-100: 4 months

OVR-100 / OVCM-100 : HEPES

ODT×10 / OCS10 / Square Measure

Endotoxin 侓0.5EU / unit(EU/mL) / Mouse embryo assay 侔80ˍ
Storage: 15-30ˈ
Shelf Life: 3 years

PROTOCOL
Vi trific atio n01

Wash an ovary with Ova RinseʤREF. 82215ʥ.
Use a surgical knife and to cut it into dimensions of
10 x 10 x 1mm. Take care to maintain tissue wet
by using Ova Rinse throughout the process.

Square Measure
ovarian tissue(10×10×1mm)

Note: By usnig Square Measure（REF. 81212）,
the ovary can be easily cut to appropriate size.

02 Equilibrate the ovarian tissues cut into appropriate sizes with Ova Cryo Kit Type M（REF. 82212）prepared

to room temperature; equilibrate it in Cryo1 for 5 minutes, Cryo2 for 5 minutes, and Cryo3 for 15 minutes.

Note: Before transferring to the next media, gently wipe the ovarian tissue with gauze to minimize previous media.

Cryo1

5min

Cryo2

5min

Gauze

Cryo 3

15min

Gauze

03 After the equilibration in Cryo3, wipe the ovarian tissue with gauzeand place it on the cryodevice maximizing its surface area.

AB

Open System

Closed SystemAɻOpen SystemʁOva Cryo Device Type MʤREF. 81213ʥ

Ova Cryo Sheetɻ Place the ovarian tissue on its metal part.

BɻClosed SystemʁOva Cryo SheetʤREF. 81214ʥ

Ova Cryo DeviceType M

ɻ Flip its film and place the ovarian tissue on it.
ɻ Flip back the film, expel air using fingers or tweezers.
ɻ Insert it into the pouch, expel air, andseal the pouch at the black line using a heat sealer.
ɻ04

SEAL

No:
ID:
Day:

Extra pouch
for Ova Cryo SheetAA Plunge the cryodevice into liquid nitrogen,Bthen close the cap.

No:
ID:
Day:B Plunge the cryodevice into liquid nitrogen.
Liquid nitrogen

05

A Place the cryodevice on the cane and store it in a tank.
B Place the cryodevice toOva Cryo Sheet CaneʤREF. 81214, 81215ʥand storeit in a tank.

Liquid nitrogenAB

Ova Cryo Sheet CanePROTOCOL
T h aw in g01

B

Remove the cryo device from the tank and immerse it into liquid nitrogen.

Closed System

A Open SystemʁOva Cryo Device Type Mɻɻ Open the cryodevice in liquid nitrogen.

A

No:
ID:
Day:

Open System

B Closed SystemʁOva Cryo SheetɻɻCut the sealed pouch along the red line
in liquid nitrogen.

CUT

Note: Avoid direct contact between the Cryo Sheet and liquid nitrogen.

Liquid nitrogen

02 Thaw using Ova Thawing Kit Type MʤREF. 82222ʥ.

AA Plunge the cryodevice into Thaw1 warmed to 37ˈ

B

in the water bath.
Shake it in thaw1 to detach the ovarian tissue.
B Remove the cryodevice from the pouch and transfer it

into Thaw1 brought to 37ˈ in the water bath.

Thaw1
ʤ37ˈʥ

03 Transfer the tissue from Thaw1 to Thaw2 at room temperatureand leave it for 3min. Then, transfer the tissue from Thaw2 toThaw3 at room temperature and leave it for 5min.

1min

Thaw2

Thaw 33min

5min

04 After 5 min in Thaw3, culture the tissue for recovery before transplantion.
ʕIf transplant immediately after thaw, culture in Ova Culture with HEPES（REF. 82217）for 30 min.
ʕFor recovery culture more than 30 min, use Ova Culture（REF. 82216）in an incubator.

Specification may change without pre-notice for purpose of product improvement.

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