OYC Research Reagent

IDO-MCA
Anti-Human Indoleamine 2,3-Dioxygenase Monoclonal Antibody
Indoleamine 2,3-dioxygenase: IDO locates many organs ; human lung,intestine, placenta,… and it is heavily induced by inflammation and infectiondiseases to accelerate hypermetabolism of Tryptophan. IDO plays animportant role in immune system, especially for immune tolerance
establishment by inducing suppressive T-cell.
IDO is also highly expressed in tumor cell to inactivate Effecter cell and NK
cell, in order to escape from the elimination action by immune defense system.
IDO is highlighted as possible tumor prediction marker.
In contrast, IDO induction in central nave products neurotoxin, quinoline acidand is suspected involvement of neurodegenerating diseases, such as
Alzheimer and AIDS cognition disorder.
The product is produced by hybridoma cell, which is developed from purified
human-IDO antigen immunized mice spleen, and cultivated in Serum-Free
Medium Culture and purified by Protein G affinity chromatography.

Specifications
Purified-Human-IDO

Subclass

IgG1

Concentration

1 mg/mL

Solvent

PBS (NaN3 0.05%)

Content100 µg

DeliveryFrozenApplicationsImmunohistochemical Staining （available for paraffin section）,
Immunoprecipitation , Western blot , ELISA

Date
312345672.5

A450

www.oyc.co.jp

Antigen

IDO
45kDa

21.5
1

IDO expression inspection
in ovarian cancer（western blot）

0.5
00.01 0.1

1

10

100 1000 10000

Concentration of antibody(mg/ml)

Lane1 : Positive Control（placentra）
Lane2-7 : ovarian cancer sample

ELISA by purified-human IDO
Use purified- human IDO solidphase(2mg/ml, 100mｌ/well),
Add serial dilution IDO-MCA(100mｌ/well).
Use HRP-conjugated anti-mouse IgG for
secondary antibody,
And measured absorbance (450nm)

Data：Dr. Kazuhiko INO, NAGOYA Uni.

OYC Research ReagentA

BCDEFIDO immunohistochemical staining of Endometorial cancer
A, B, C, D: endometrial cancer sample
E:endometrial cancer IDO negative control （Use mice normal IgG replace for IDOMCA）
F:placentra IDO positive control
Data：Dr. Kazuhiko INO, NAGOYA Uni.

References

www.oyc.co.jp

1. Takikawa O. Biochemical and medical aspects of theindoleamine 2,3-dioxygenase-initiated L-tryptophan
metabolism. Biochem Biophys Res Commum.2005 Dec
9;338(1):12-9. Review.
2.Mellor AL, Munn DH. IDO expression by dendritic cells :tolerance and tryptophan catabolism. Nat RevImmunol.2004 Oct;4(10):762-74. Review.
3. Uyttenhove C. et al. Evidence for a tumoral immune
resistance mechanism based on tryptophan degradation
by indoleamine 2,3-dioxygenase. Nat Med.2003
Oct;9(10):1269-74.
4.Munn DH, Mellor AL. Indoleamine 2,3-dioxygenase andtumor-induced tolerance. J Clin Invest.2007
May;117(5):1147-54.Rview.
5.Della Chiesa M. et al.The tryptophan catabolite Lkynurenine inhibits the surface expression of NKp46-and
NKG2D-activating receptors and regulates NK-cellfunction. Blood.2006 Dec 15;108(13):4118-25.

6.Okamoto A. et al. Indoleamine 2,3-dioxygenase serves as amarker of poor prognosis in gene expression profiles ofserous ovarian cancer cells. Clin Cancer Res. 2005 Aug
15;11(16):6030-9.
7.Ino K. et al. Indoleamine 2,3-dioxyganase is a novel
prognostic indicator for endometrial cancer. Br JCancer.2006 Dec 4;95(11):1555-61.
8.Nakamura T. et al. Expression of indoleamine 2,3-dioxyganaseand the recruitment of Foxp3-expressing regulatory T cells inthe development and progression of uterine cervical cancer.
Cancer Sci. 2007 Jun;98(6):874-81.
9.Kwidzinski E, Bechmann I. IDO expression in the brain: adouble-edged sword. J Mol Med. 2007 Jun 27.
10.Guillemin GJ. et al. Indoleamine 2,3 dioxygenase andquinolinic acid immunoreactivity in Alzheimer’s diseasehippocampus. Neuropathol Appl Neurobiol. 2005
Aug;31(4):395-404.

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