近畿大学次世代基盤技術研究所報告 Vol. 7（2016）59－63⣽⬊⼥ྜ⏝ᇶᯈ࡜ࡋ࡚ࡢ⣽⬊࣐࢖ࢡࣟ࢔ࣞ࢖࡬ࡢ PEG ᅛᐃ໬࡜⾲㠃ᛶ≧ࡢホ౯௒ᇛ ᫂඾*1㸪Ọᒸ ᚿ✑*2㸪ᓲ ※↵*3, Ọᓥ ṇႹ*3, ⓑ▼ ᾈᖹ*4
Immobilization of PEG onto cell microarrays as a substrate for cell fusionand evaluation of their surface properties
Akinori IMAJO*1㸪Shiho NAGAOKA*2㸪Wonhwan CHOI*3㸪
Masatsugu NAGASHIMA*3, and Kohei SHIRAISHI*4


To prepare a surface-modified microarray(μAy) on both PEG segments and specific protein
binding sites as a substrate for high-efficiency cell fusion, PEG macromonomer(macPEG)[P(macPEG)] or/and N-succinimidyl arylate(NAS) and macPEG copolymer [P(macPEG-coNAS)] with different compositions were selectively immobilized on the glass spots of the μAy by
using surface-initiated radical copolymerization. Two kinds of selective immobilization methodsof 3-aminopropyltrimethoxysilane were attempted on a glass substrate as a model of spots ofμAy by using 1)vapor deposition and 2)immersion in the solution, and evaluated their surfaces
from the scanning probe microscope(SPM) and water contact angle measurements.
Poly(macPEG) and poly- (macPEG-co-NAS) chains as brushes were observed on theglass(g-glass). As a result, the SPM images and wettability based on the immobilized polymers
on the g-glasses were changed by changing treated methods of alkoxysilane.
Keywords: PEG immobilization, Suface-initiated radical polymerization, Cell microarray,
Cell fusion, Vapor deposition method ࡣࡌࡵ࡟య⣽⬊࠿ࡽㄏᑟྍ⬟࡜࡞ࡗࡓேᕤከ⬟ᛶᖿ⣽⬊(iPS)⣽⬊ࡢ㛤Ⓨ࡟ࡼࡾ㸪ձ஦ᨾࡸ⑓Ẽ➼࡛ኻࢃࢀࡓ⤌⧊ࢆ⮬㌟ࡢᖿ⣽⬊ࢆ౑ࡗ࡚ᙧࡸᶵ⬟ࢆ෌⏕ࡍࡿ་⒪ᢏ⾡(1)㸪ղ⑌ᝈࡢᶵᗎゎ᫂ࡸ἞⒪ἲ㑅ᢥ(2),(3)㸪ճDNAࣜࣉࣟࢢ࣑ࣛ
ࣥࢢ(4)ࡸᖿ⣽⬊◊✲➼࡬ࡢᵝࠎ࡞ᛂ⏝ࡀ21ୡ⣖ࡢ᭱ඛ➃་⒪ཬࡧ⛉Ꮫᢏ⾡➼ࡢ㣕㌍ⓗ࡞Ⓨᒎࡀᮇᚅࡉࢀ㸪ゅ⭷⛣᳜࡛ࡣᐇ⏝໬ࣞ࣋ࣝ࡜࡞ࡗ࡚࠸ࡿ(1)㸬ࡲࡓ㸪iPS⣽⬊࠿ࡽศ໬ㄏᑟࡉࢀࡿ⣽⬊࡟ࡼࡗ࡚㸪⮚ჾ≉␗ⓗ࡞ᝈ⪅⮬㌟ࡢ⣽⬊ࡢධᡭࡀྍ⬟࡜࡞ࡾ㸪࣮࢜ࢲ࣮࣓࢖ࢻ࡞⸆๣᥈⣴㸪᪂⸆㛤Ⓨࡢ᪂ࡋ࠸࣮ࣝࢺᙧᡂཬࡧ㛵㐃◊✲ࡀ῝໬ࡋ
ຍ㏿ࡍࡿ࡜⪃࠼ࡽࢀࡿ(5)㸬ࡉࡽ࡟㸪᪂⸆㛤Ⓨࢫࢡ࣮ࣜࢽࣥࢢ࡛ࡢື≀ᐇ㦂ࡢ㍍ῶ㸪ᶵ⬟ᛶ㣗ရ㸪⏕⌮άᛶ≀㉁㸪

⎔ቃ࣍ࣝࣔࣥࡢ᥈ᰝࡢࣁ࢖ࢫ࣮ࣝࣉࢵࢺホ౯➼ከ᪉㠃࡬ࡢἼཬࡀ⪃࠼ࡽࢀ㸪ከࡃࡢ᪂⏘ᴗ๰ฟࡶぢ㎸ࡲࢀ࡚࠸
ࡿ(6)㸬
ࣄࢺேᕤᰁⰍయ(HAC)ᢏ⾡(7)ࡣ㸪ձᕧ኱㑇ఏᏊ⩌ࡢᑟධ㸪ղᐟ୺ᰁⰍయୖࡢ㑇ఏᏊࢆ◚ቯࡋ࡞࠸㸪ճᑟධ㑇ఏᏊࡢ㐣๫Ⓨ⌧ࡸⓎ⌧ᾘኻࡀ㉳ࡁࡎⓎ⌧ࡀኚ໬ࡋ࡞࠸㸪մᑟධ㑇ఏᏊࡢⓎ⌧ࢆ⮬⏤ไᚚ࡛ࡁࡿ➼ࡢ≉㛗ࢆࡶࡕ㸪iPS ⣽⬊స〇(8)ࢆጞࡵ㸪ᏳᚰᏳ඲࡞෌⏕་⒪ᢏ⾡ࡢ㸯ࡘ࡛ࡶ࠶ࡿ㸬ࡑࡢ௚㸪ࣄࢺᆺᐇ㦂ື≀ࡢస〇㸪➽ࢪࢫࢺࣟࣇ࢕࣮➼ࡢᕧ኱㑇ఏᏊࡢḞᦆ἞⒪➼ࡢᛂ⏝◊✲(9),(10)ࢆጞࡵ㸪ඛ➃་⒪ࢆᢸ࠺ᢏ⾡࡜ࡋ࡚ከ኱࡞ὀ┠ࢆࡉࢀ࡚࠸ࡿ㸬୍᪉㸪
HAC ࣋ࢡࢱ࣮ࡣᚤᑠ᰾⣽⬊ ࣑ࢡࣟࢭࣝ㸸MC ࡜ࡋ࡚⏕ᡂࡋ㸪ཷᐜ⣽⬊࡜ࡢ⣽⬊⼥ྜ࡟ࡼࡿ⛣ධ ᚤᑠ᰾⣽⬊⼥ྜཎ✏ཷ௜ 2016 ᖺ 5 ᭶ 7 ᪥*1 ㏆␥኱Ꮫ኱Ꮫ㝔 ࢩࢫࢸ࣒ᕤᏛ◊✲⛉ ࢩࢫࢸ࣒ᕤᏛᑓᨷ 㸦ࠛ739-2116 ᮾᗈᓥᕷ㧗ᒇ࠺ࡵࡢ㎶ 1 ␒㸧
*2 ㏆␥኱ᏛᕤᏛ㒊 ໬Ꮫ⏕࿨ᕤᏛ⛉ 㸦ࠛ739-2116 ᮾᗈᓥᕷ㧗ᒇ࠺ࡵࡢ㎶ 1 ␒㸧
*3 ࢚ࢫࢸࢵࢡᰴᘧ఍♫ ᢏ⾡㒊 㸦ࠛ699-0101 ᓥ᰿┴ᯇỤᕷᮾฟ㞼⏫ᥬᒇ 2797-3㸧
*4 ㏆␥኱Ꮫ኱Ꮫ㝔 ࢩࢫࢸ࣒ᕤᏛ◊✲⛉ ࢩࢫࢸ࣒ᕤᏛᑓᨷ ᩍᤵ㸪ᕤᏛ㒊 ໬Ꮫ⏕࿨ᕤᏛ⛉ ᩍᤵ㸪ḟୡ௦ᇶ┙ᢏ⾡◊✲ᡤ ᩍᤵ㸦ࠛ739-2116 ᮾᗈᓥᕷ㧗ᒇ࠺ࡵࡢ㎶ 1 ␒㸧㐃⤡ඛ㸸ⓑ▼ᾈᖹ㸦◊✲௦⾲⪅㸧E-mail siraisi@hiro.kindai.ac.jp－ 59 －

ἲ㸸MMCT ࡢࡓࡵ㸪ᚑ᮶ἲ࡛᭱኱࡛ 0.0001㸣࡜⼥ྜຠ⋡ࡣᴟࡵ࡚ప⋡࡛࠶ࡿ㸬ࣄࢺ⣽⬊⭷ୖࡢཷᐜయ⤖ྜᛶࡢ㯞⑈࢘࢖ࣝࢫ࢚࣮ࣥ࣋ࣟࣈࢱࣥࣃࢡ㉁࡛ฎ⌮ࡋࡓ MC(11)ࢆ⏝࠸࡚⼥ྜຠ⋡ࡢྥୖࡀ᳨ウࡉࢀ࡚࠸ࡿࡀ㸪⼥ྜຠ⋡ࡣ 0.001%⛬ᗘ࡜ࡉࡽ࡞ࡿྥୖࡀồࡵࡽࢀࡿ㸬ࡲࡓ㸪㯞⑈࢘࢖ࣝࢫ⾲㠃ᥦ♧ἲࡣ㸪ࣄࢺᆺ⣽⬊࡟㝈ᐃࡉࢀࡿࡓࡵ㸪௚⣽⬊✀࡬ࡢỗ⏝ᛶࡢ㧗࠸ᡭἲࡶồࡵࡽࢀࡿ㸬᪤Ꮡࡢ⣽⬊⼥ྜᢏ⾡(12)࡜ࡋ࡚▱ࡽࢀ࡚࠸ࡿ㧗⃰ᗘࡢ࣏࢚ࣜࢳࣞࣥ
ࢢࣜࢥ࣮ࣝ(PEG)ࢆ⏝࠸ࡓ PEG ἲ(13)~(17)ࡸ㟁Ẽ⼥ྜἲ(18)~(22)ࡣ 2 ✀ࡢ⣽⬊ࢆࣛࣥࢲ࣒࡞ΰྜ≧ែ࡛⼥ྜࡍࡿࡓࡵ㸪┠ⓗ࡜ࡍࡿ 2 ⣽⬊ࡀ⼥ྜࡍࡿ஦ࡣᴟࡵ࡚അⓎⓗ࡛࠶ࡾ㸪ྠ✀ࡢ⣽⬊ࡸ 3 ಶ௨ୖࡢ⣽⬊ࡢ⼥ྜ㸪඲ࡃ⼥ྜࡋ࡞࠸⣽⬊ࡀከᩘࢆ༨ࡵࡿ㸬ࡲࡓ㸪⼥ྜຠ⋡ࡸ⣽⬊࡬ࡢࢲ࣓࣮ࢪࡲࡓࡣ㸪౑⏝࡛ࡁࡿ⣽⬊✀࡞࡝࡟ࡶ㝈⏺ࡀ࠶ࡿࡇ࡜࡞࡝ࡀㄢ㢟࡜ࡋ࡚ᣲࡆࡽࢀ࡚࠸ࡿ㸬ᚑ᮶ἲ࡛ࡣ㸪⣽⬊㸫⣽⬊㛫ࡢ᥋ゐࡸ⭷⼥ྜࢆಁ㐍ࡉࡏࡿࡓࡵ㸪ከᩘࡢ HACྵ᭷ MC ࡜ཷᐜ⣽⬊ࢆ౑⏝ࡋ࡚㸪30%㹼50%ࡢ㧗⃰ᗘ࣏࢚ࣜࢳࣞࣥࢢࣜࢥ࣮ࣝ(PEG)Ỉ⁐ᾮ୰࡛㸪ᩘศ㛫࡟㝈ᐃࡋ࡚⣽⬊⼥ྜࡋ࡚࠸ࡿ㸬ཷᐜ⣽⬊࡜ MC ࡢ᥋ゐࢆ㧗ࡵ㸪⣽⬊࡬ࡢࢲ࣓࣮ࢪࡢ኱ࡁ࠸㧗⃰ᗘ PEG ⁐ᾮࡢ౑⏝ࢆ㑊ࡅ㸪⼥ྜຠ⋡ࢆ㧗ࡵࡿࡇ࡜ࡣ HAC ᢏ⾡ࢆⓎᒎࡉࡏࡿࡓࡵ࡟᭱ࡶ㔜せ࡞ㄢ㢟ࡢ 1 ࡘ࡛࠶ࡿ㸬ᮏ◊✲࡛ࡣ㸪⼥ྜຓ๣࡜ࡋ࡚ࡢ PEG 㙐ࢆ⣽⬊᥋╔㠃࡟ᅛ┦໬ࡋࡓ࣐࢖ࢡࣟ࢔ࣞ࢖(μAy)ࢆㄪ〇ࡋ㸪μAy ࡬ࡢ⣽⬊㞟✚࡟ࡼࡿ MC ࡜ཷᐜ⣽⬊ࡢ᥋ゐຠ⋡ࡢቑ኱࡜㸪᥋╔㠃࡛ࡢ PEG 㙐࡟ࡼࡾ㸪ప࣭㠀౵くⓗ࡞⼥ྜಁ㐍ࢆ┠ⓗ࡜ࡋࡓ㸬ᅛ┦໬ PEG ࡛ࡣ㸪㞟✚ࡋࡓ⣽⬊᥋ゐ㠃ࡢࡳ࡛⣽⬊ᠱ⃮ᾮ඲యࡣ㸪㏻ᖖࡢᇵ㣴᮲௳࡛࠶ࡿࡓࡵ㸪㛗᫬㛫ࡢ⼥ྜࡶྍ⬟࡛࠶ࡿ㸬ࡉࡽ࡟㸪⣽⬊⼥ྜࡀಁ㐍ࡉࢀࡿ μAy ᇶᯈୖ࡛ࡣ㸪⣽⬊ࢧ࢖ࢬࡼࡾࡶᚤᑠ࡜࡞ࡿ㑇ఏᏊࡸࢱࣥࣃࢡ㉁➼ࡢ≀㉁ࡢ⛣ධࡣࡉࡽ࡟ᐜ࡛᫆࠶ࡾ㸪⣽⬊ෆ≀㉁ᑟධᇶᯈ࡜ࡋ࡚ࡢᛂ⏝ࡶྍ⬟࡛࠶ࡿ࡜⪃࠼ࡽࢀࡿ㸬ࡇࡢ࡜ࡁ㸪μAy ᇶᯈୖࡢ⣽⬊᥋╔㠃ࡢ࢞ࣛࢫࢫ࣏ࢵࢺ࡟࢞ࣛࢫ≉␗ⓗ࡞ࢩࣛࣥ࢝ࢵࣉࣜࣥࢢ๣ࢆᅛᐃ໬ࡍࡿ㸬ศᏊ㸪ᚤ⢏Ꮚ㸪ࢡࣛࢫࢱ࣮࡞࡝ࡢᚤᑠせ⣲ࡀ⮬Ⓨⓗ࡟㞟ྜࡋ㸪つ๎ⓗ࡞㓄ิࢆᙧసࡿࡇ࡜ࢆ⮬ᕫ㞟✚໬⌧㇟࡜࠸࠸㸪ࢩࣛࣥ࢝ࢵࣉࣜࣥࢢ๣ࡢᅛᐃ໬࡟ࡣࡇࡢ⌧㇟ࢆ฼⏝ࡋࡓ㸬ᅛᐃ໬᪉ἲ࡜ࡋ࡚⵨╔ἲ㸦Ẽ┦ἲ㸧ࡸᾐₕἲ㸦ᾮ┦ἲ㸧➼ࡀ࠶ࡿ㸬⵨╔ἲࡢᾐₕἲ࡜ẚ㍑ࡋࡓሙྜࡢ฼Ⅼ࡜ࡋ࡚㸪⁐፹ࢆ౑ࢃ࡞࠸ࡓࡵ࡟ᗫᾮࡀฟ࡞࠸ࡇ࡜㸪᭷ᶵࢩࣛࣥศᏊࡀ㔜ྜࡋ࡚࡛ࡁࡿ⢏Ꮚ≧ࡢሁ✚≀ࡀᑡ࡞࠸ࡇ࡜ࡀᣲࡆࡽࢀ(23)㸪ᮏἲ࡛ࡣࡇࡢ⵨╔ἲ࡟╔┠ࡋ㸪ᅛ┦໬ࡍࡿ PEG ࢭࢢ࣓ࣥࢺࡢ㧗ᐦᗘ໬ࢆ┠ᣦࡋࡓ㸬ࡑࡢᚋ㸪 PEG ࢆᅛ┦໬ࡍࡿ
࡜ඹ࡟㸪PEG ࢭࢢ࣓ࣥࢺ୰࡟ࡣ㸪⣽⬊⭷ୖࡢࢱࣥࣃࢡ㉁ࡢ NH2 ᇶ࡜ࡢ཯ᛂ㒊఩ࡀ࠶ࡿࢫࢡࢩࣥ࢖࣑ࢻᇶࢆࡶࡘ
N-succinimidyl acrylate(NAS)ࢆᑟධࡋࡓ㸬PEG ࢭࢢ࣓ࣥࢺࢆഃ㙐ࡶࡘ࣓ࢱࢡࣜࣝ㓟⣔࣐ࢡࣟࣔࣀ࣐࣮
Poly(ethylene glycol)methyl ether methacrylate(Mn:1,100)[macPEG] ࢆ ᅛ ┦ ໬ ࡋ ࡓ P(macPEG) ཬ ࡧP(macPEG-co-NAS)ࢢࣛࣇࢺ໬࢞ࣛࢫཬࡧμAy ࢆㄪ〇ࡋࡓ
✀ㄪ〇ࡋࡓ g-ǍAy ࢆ㉮ᰝᆺࣉ࣮ࣟࣈ㢧ᚤ㙾(SPM) ᐃཬࡧỈ᥋ゐゅ(ǉ) ᐃ࠿ࡽホ౯ࡋࡓ㸬 ᐇ㦂᪉ἲ ヨ⸆◲㓟 ࿴ග⣧⸆ओ〇 㸪㐣㓟໬Ỉ⣲ 㛵ᮾ໬Ꮫओ〇 㸪
3-aminopropyltrimethoxysilane[APMSi] ᮾ ி ໬ ᡂ ओ〇 㸪N,N-dimethylformamide ⬺Ỉ [DMF] ࿴ග⣧⸆ओ〇 㸪triethylamine[TEA] ࿴ග⣧ओ〇 ࢆሷ໬࣒࢝ࣜ࢘࡟ࡼࡗ࡚⬺Ỉࡋࡓࡶࡢࢆ⏝࠸ࡓ㸬NAS ᮾி໬ᡂओ〇 㸪
macPEG (Mn: 1,100) $OGULFK 〇 ࡣࢪ࢚ࢳ࢚࣮ࣝࢸࣝ࡟⁐ゎᚋ࡟㸫80Υ࡟෭༷ࡋ࡚෌ỿẊ࡟ࡼࡗ࡚⢭〇ࡋࡓ㸬࣐࢖ࢡࣟ࢔ࣞ࢖(μAy)ࡣࢺ࣮࣮࢚ࣚ࢖ࢸࢵࢡओ〇ࢆ⏝࠸ࡓ.Ỉࡣ࣑࣏ࣜ࢔〇 Milli-Q synthesis ᆺ㉸⣧Ỉ〇㐀⿦⨨ࢆ⏝࠸࡚⢭〇ࡋ࡚౑⏝ࡋࡓ㸬࢚ࢱࣀ࣮ࣝ㸪ࢡ࣒ࣟࣟ࣍ࣝࡢ⁐፹ࡣ㸪ᖖἲ࡟ࡋࡓࡀࡗ࡚⢭〇ࡋࡓ㸬
4,4’-azobis(4-cyanovaleric acid chloride)[V501-Cl] ࡣ
4.4’-azobis(4-cyanovaleric acid)[V501] ࿴ග⣧⸆ओ〇 ࡜஬ሷ໬ࣜࣥ[PCl5] ࢟ࢩࢲ໬Ꮫओ〇 ࠿ࡽྜᡂࡋࡓ㸬 ᾐₕἲ࡜⵨╔ἲࢆ⏝࠸ࡓPDF3(*ࢢࣛࣇࢺ໬࢞ࣛࢫ>SRO\ PDF3(* JJODVV@࡜PDF3(*1$6ඹ㔜ྜయࢢࣛࣇࢺ໬࢞ࣛࢫ>SRO\ PDF3(*FR1$6 JJODVV@ࡢㄪ〇࢞ࣛࢫ〇ࢩ࣮ࣕࣞࢆ⏝࠸㸪ࣆࣛࣥࣁ⁐ᾮ[30㸣㐣㓟໬Ỉ
⣲/18 M ◲㓟=1/3(v/v)] 8 mL ࡟࢝ࣂ࣮࢞ࣛࢫ(FisherBlan)ࢆධࢀ1 h ᾐₕࡋ㸪Ỉ࡛࢝ࣂ࣮࢞ࣛࢫࢆὙίࡋࡓᚋ㸪࢚࢔࣮ࢥࣥࣉࣞࢵࢧ࣮࡛✵Ẽࢆ྿ࡁ௜ࡅ࡚஝⇱ࡋࡓ㸬[APMSi ᅛᐃ໬࢞ࣛࢫࡢㄪ〇]ᾐₕἲ㸸0.179 g (1 mmol) APMSi ࡢ DMF ⁐ᾮ 2 mL ࡀධࡗࡓ࢞ࣛࢫ〇ࢫࢡ࣮ࣜࣗ⟶࡟࢝ࣂ࣮࢞ࣛࢫࢆධࢀ㸪60Υ㸪3 h ཯ᛂࡋࡓ㸬ࡑࡢᚋ㸪DMF ࡛ 15 min ⾲㠃ࢆὙίࡋࡓ(Fig.1-A)㸬
⵨╔-ྞୗࡆἲ㸸ᗏࡀ㞃ࢀࡿ⛬ᗘࡢ APMSi ࢆ〓Ⰽ⎼࡟ධࢀ㸪〓Ⰽ⎼ୖ㒊࡟ࣆࣛࣥࣁฎ⌮ࢆ⾜ࡗࡓ࢞ࣛࢫᇶᯈࢆࢡࣜࢵࣉ➼⏝࠸࡚ྞୗࡆ⵹ࢆࡋ㸪ᖖ ࡟࡚ 2 h 㟼⨨ࡋ㸪཯ᛂࡋࡓ(Fig.1-B)㸬⵨╔-㈞௜ࡅἲ㸸࢞ࣛࢫ〇ࢩ࣮ࣕࣞ࡟ 3 mL ࡢ APMSi ࢆධࢀ㸪⵹㒊ศࡢෆഃ࡟୧㠃ࢸ࣮ࣉ࡟࡚ࣆࣛࣥࣁฎ⌮ࢆ⾜ࡗࡓ࢞ࣛࢫᇶᯈࢆᅛᐃࡋ㸪30Υ࡛ 2 h 㟼⨨ࡋ㸪཯ᛂࡋࡓ(Fig.1-C)㸬
⵨╔-㟼⨨ἲ㸸࢞ࣛࢫ〇ࡢࢩ࣮ࣕࣞ࡟ 3 mL ࡢ APMSi ࢆ࡜ࡾ㸪ࣆࣛࣥࣁฎ⌮ࢆ⾜ࡗࡓ࢞ࣛࢫᇶᯈ࡜ࢩ࣮ࣕࣞࢆྠᐦ㛢ࡉࢀࡓᐜჾ࡟ධࢀ㸪80Υ࡛ 2 h 㟼⨨ࡋ㸪཯ᛂࡋࡓ(Fig.

－ 60 －1-D)㸬࠸ࡎࢀࡶᅛᐃ໬ᚋࡣ 80Υࡢ஝⇱ᶵ࡟࡚ 30 min ஝⇱ࡉࡏࡿࡇ࡜࡟ࡼࡾ↝ࡁ௜ࡅࢆ⾜࡞ࡗࡓ㸬[㔜ྜ㛤ጞ๣ᅛᐃ໬]࢞ࣛࢫ〇ࢫࢡ࣮ࣜࣗ⟶࡟࢔ࢰ㛤ጞ๣ 0.0315 g(0.1 mmol)
V501-Cl ࢆ 1 mL ࡢࢡ࣒ࣟࣟ࣍ࣝ࡟⁐ゎࡋ㸪
30.6 ǍL TEAࢆຍ࠼ࡓ㸬ࡑࡢΰྜࡋࡓࢡ࣒ࣟࣟ࣍ࣝ⁐ᾮ࡟ APMSi ᅛᐃ໬࢞ࣛࢫࢆᾐࡋ㸪ᜏ ᵴ࡛ 30Υ㸪20 h ཯ᛂࡋࡓ㸬཯ᛂ⤊஢ᚋ㸪ࢡ࣒ࣟࣟ࣍ࣝ࡜࣓ࢱࣀ࣮ࣝ࡟ᾐࡋ㸪ᐊ ࡛㉸㡢ἼὙίࡋࡓ㸬
[⾲㠃㛤ጞࢢࣛࣇࢺ㔜ྜ]࢞ࣛࢫ〇ࢫࢡ࣮ࣜࣗ⟶࡟ macPEG 1.65 g(1.5 mmol)ࡣ࢚
ࢱࣀ࣮ࣝ⁐ᾮ 1 mL ຍ࠼, ࡲࡓ㸪macPEG 0.99 g(0.9mmol)࡜NAS 0.101 g(0.6 mmol)ࡣ0.5 mL ࢚ࢱࣀ࣮ࣝ࡜
0.5 mL DMF ࡢ 1㸸1 ΰྜ⁐ᾮ 1 mL ຍ࠼㸪V501-Cl ᅛᐃ໬࢞ࣛࢫࢆᾐࡋࡓ㸬ᾮయ❅⣲ᾎ୰࡛෾⤖㸫⬺Ẽ㸫❅⣲⨨᥮ࢆ⧞ࡾ㏉ࡋࡓᚋ㸪ᜏ ᵴ࡛ 80Υ㸪20 h 㔜ྜࡋࡓ㸬㔜ྜᚋ㸪࢚ࢱࣀ࣮ࣝ࡟ᾐࡋ㸪ᐊ ࡛ 3 ᅇ㸪45 min ㉸㡢ἼὙίࡋࡓᚋ㸪┿✵஝⇱ࡋ PEG ᅛᐃ໬ g-glass ࢆᚓࡓ㸬⟶࡟࢔ࢰ㛤ጞ๣ 0.0315 g(0.1 mmol)V501-Cl ࢆ 1 mL ࡢࢡ࣒ࣟࣟ࣍ࣝ࡟⁐ゎࡋ㸪30.6 ǍLTEA ࢆຍ࠼ࡓ㸬ࡑࡢΰྜࡋࡓࢡ࣒ࣟࣟ࣍ࣝ⁐ᾮ࡟ APMSi ᅛᐃ໬࢞ࣛࢫࢆᾐࡋ㸪ᜏ ᵴ࡛ 30Υ㸪20 h ཯ᛂࡋࡓ㸬཯ᛂ⤊஢ᚋ㸪ࢡࣟࣟ࣍ࣝ
࣒࡜࣓ࢱࣀ࣮࡛ࣝ㉸㡢ἼὙίࡋࡓ㸬࢞ࣛࢫ〇ࢫࢡ࣮ࣜࣗ⟶࡟ macPEG 1.65 g(1.5 mmol) ࡜࢚ࢱࣀ࣮ࣝ⁐ᾮ 1 mLຍ࠼㸪V501-Cl ᅛᐃ໬࢞ࣛࢫࢆᾐࡋ㸪ᾮయ❅⣲ᾎ୰࡛෾⤖㸫⬺Ẽ㸫❅⣲⨨᥮ࢆ⧞ࡾ㏉ࡋࡓᚋ㸪ᜏ ᵴ࡛ 80Υ㸪20h 㔜ྜࡋࡓ㸬㔜ྜᚋ㸪࢚ࢱࣀ࣮ࣝ࡟ᾐࡋ㸪ᐊ ࡛ 3 ᅇ㸪
45 min ㉸㡢ἼὙίࡋࡓᚋ㸪┿✵஝⇱ࡋ g-μAy ࢆᚓࡓ㸬

⵹ࡢෆഃFig.2. ⵨╔-㈞௜ࡅἲ࡟ࡼࡿ μAy ࡬ࡢ APMSi ࡢᅛᐃ໬(μAy:ᅗ୰㛗᪉ᙧ) JJODVV ཬࡧ Jw$\ ⾲㠃ࡢ࢟ࣕࣛࢡࢱࣜࢮ࣮ࢩࣙࣥ  Ỉ࡟ᑐࡍࡿ᥋ゐゅࡣ SImage Entry ࢚࢟ࢩ࣐ओ〇 ࢆ⏝࠸࡚ᐊ (25Υ)࡛ ᐃࡋࡓ㸬ᇶᯈୖ࡟ 10 μL ࡢỈ⁲ࢆ⨨࠸ࡓ㸬1 min ௨ෆ࡟Ỉ⁲ࡢᕥྑࡢ᥋ゐゅࢆ ᐃࡋࡓ㸬ᐃࡣィ 5 ᅇࡢ࠺ࡕ㸪᭱኱್ཬࡧ᭱ᑠ್ࢆ㝖࠸ࡓᖹᆒࢆ᥋ゐゅ࡜ࡋࡓ㸬ࡲࡓ㸪㉮ᰝᆺࣉ࣮ࣟࣈ㢧ᚤ㙾(SPM)ࡢ
ᐃࡣ Shimadzu 〇 SPM-9500J3 ᆺ ࢝ࣥࢳࣞࣂ࣮࡟
Innovation Solutions Bulgaria Ltd㸬〇 Budget sensors
Tap300AI-G ࢆ⏝࠸㸪኱Ẽ㞺ᅖẼ୰࡛㸪㉮ᰝ⠊ᅖ㸸30
μm×30 μm㸪㉮ᰝ㏿ᗘ㸸0.5 Hz ࡜ࡋ㸪఩┦᳨ฟࢩࢫࢸ࣒࡟ࡼࡿ⾲㠃ࡢ㧗ప ᐃཬࡧ⢓ᙎᛶ ᐃ࠿ࡽホ౯ࡋࡓ㸬

Fig.1. ᾐₕἲ࡜⵨╔ἲ࡟ࡼࡿ࢞ࣛࢫᇶᯈ࡬ࡢAPMSiࡢᅛᐃ໬㸦࢞ࣛࢫᇶᯈ㸸ᅗ୰෇㸧 ⵨╔㈞௜ࡅἲࢆ⏝࠸ࡓ PDF3(* ࢢࣛࣇࢺ໬࢔ࣞ࢖>SRO\ PDF3(* Jw$\@ࡢㄪ〇࢞ࣛࢫ〇ࢩ࣮ࣕࣞࢆ⏝࠸㸪ࣆࣛࣥࣁ⁐ᾮ[30㸣㐣㓟໬Ỉ
⣲/18 M ◲㓟=1/3(v/v)]8 mL ࡟࢝ࣂ࣮࢞ࣛࢫࢆධࢀ 1 hᾐₕࡋ㸪Ỉ࡛࢝ࣂ࣮࢞ࣛࢫࢆὙίࡋࡓᚋ㸪࢚࢔࣮ࢥࣥࣉࣞࢵࢧ࣮࡛✵Ẽࢆ྿ࡁ௜ࡅ࡚஝⇱ࡋࡓ㸬⵨╔-㈞௜ࡅἲ㸸࢞ࣛࢫ〇ࢩ࣮ࣕࣞ࡟ 3 mL ࡢ APMSi ࢆධࢀ㸪⵹㒊ศࡢෆഃ࡟୧㠃ࢸ࣮ࣉ࡟࡚ࣆࣛࣥࣁฎ⌮ࢆ⾜ࡗࡓ μAy ᇶᯈࢆᅛᐃࡋ㸪30Υ࡛ 2 h 㟼⨨ࡋ㸪཯ᛂࡋࡓ(Fig.2)㸬ᅛᐃ໬ᚋࡣ 80Υࡢ஝⇱ᶵ࡟࡚ 30 min ஝⇱ࡉࡏࡿࡇ࡜࡟ࡼࡾ↝ࡁ௜ࡅࢆ⾜࡞ࡗࡓ㸬࢞ࣛࢫ〇ࢫࢡ࣮ࣜࣗ ⤖ᯝ࡜⪃ᐹ ᾐₕἲ࡜⵨╔ἲࢆ⏝࠸ࡓ PDF3(* ࢢࣛࣇࢺ໬࢞ࣛࢫ>SRO\ PDF3(* JJODVV@࡜PDF3(*1$6ඹ㔜ྜయࢢࣛࣇࢺ
໬࢞ࣛࢫ>SRO\ PDF3(*FR1$6 JJODVV@ࡢ࢟ࣕࣛࢡࢱࣜࢮ࣮ࢩࣙࣥᾐₕἲ࡛ࡣ㸪APMSi ࡟ΰྜࡍࡿࡓࡵࡢ⁐፹ࡸᾐₕᚋࡢᇶᯈὙί⏝᭷ᶵ⁐፹➼ࡢᗫᾮࡢቑຍࡸὙί⏝᭷ᶵ⁐፹ࡢ஝⇱ࡀᚲせ࡛࠶ࡿ㸬ࡲࡓ㸪APMSi ࡢ᭷ᶵࢩࣛࣥศᏊࡀ㔜ྜࡋ࡚࡛ࡁࡿ⢏Ꮚ≧ࡢሁ✚≀ࡀከ࠸ࡓࡵ㸪࣏࣐࣮ࣜᅛᐃࡢ㊊ሙ࡜࡞ࡿ࢔࣑ࣀᇶࢆᐦ࡟ᅛᐃ໬࡛ࡁ࡞࠸ࡇ࡜ࡀᣲࡆࡽࢀࡿ㸬APMSi ᅛᐃ໬ࣔࢹࣝ࡜ࡋ࡚ࡣ㸪ࣆࣛࣥࣁฎ⌮ࡋぶỈᛶ࡜࡞ࡗࡓ࢞ࣛࢫᇶᯈ࡟ࢩࣛࣥศᏊࡀ྾╔ࡍࡿࡇ࡜࡟ࡼࡾ㸪ᮎ➃ࡢ࢔࣑ࣀᇶࡀ࢞ࣛࢫᇶᯈ࡬ᅛᐃ໬ࡉࢀ㸪࣏࣐࣮ࣜ㔜ྜࢆ⾜࠺ࡓࡵࡢ㛤ጞ๣ࢆᅛᐃ໬ࡍࡿࡓࡵࡢ㊊ሙ

－ 61 －࡜࡞ࡿ㸬ࡋ࠿ࡋ㸪ᾐₕἲ࡛ࡣࡇࡢࢩࣛࣥศᏊྠኈࡀ㔜࡞ࡾྜࡗ࡚㔜ྜࡍࡿྍ⬟ᛶࡀ㧗ࡃ࡞ࡾ㸪㔜ྜྍ⬟࡞ᮎ➃࢔࣑ࣀᇶࡀᇙࡶࢀ㸪ᇶᯈ⾲㠃࡟ᆒ୍࡟ᅛᐃ໬ࡋ࡚࠸ࡿ≧ែ࡜ࡣ࠸࠼ࡎ㸪⤖ᯝ࡜ࡋ࡚࢞ࣛࢫ⾲㠃࡟ APMSi ࡀከᩘᅛᐃ࡛ࡁ࡚ࡶ㸪㛤ጞ๣ࢆᅛᐃ໬ྍ⬟࡞࢔࣑ࣀᇶࡢᩘࡀῶᑡࡋ㸪ᆒ୍࡞࣏࣐࣮ࣜᅛᐃࡀ㞴ࡋ࠸࡜⪃࠼ࡽࢀࡿ㸬୍᪉㸪
APMSi ࢆ ⵨ Ⓨ ࡉ ࡏ ࡿ ࡜ 㸪 ᇶ ᯈ ⾲ 㠃 ࡟ SAM (SelfAssemble Monolayer㸧⭷࡜࿧ࡤࢀࡿ㓄ྥᛶࢆᣢࡘ⮬ᕫಟ᚟⭷ࡀᙧᡂࡉࢀࡿ㸬⵨╔ἲ࡛ࡣ㸪⁐፹ࡢ౑⏝ࡀ࡞ࡃᗫᾮࡢῶ㔞࡜࡞ࡾ㸪ᇶᯈస〇ࢥࢫࢺࡢ๐ῶࡸᇶᯈస〇᫬㛫ࡢ▷⦰ࡸᕤ⛬ࡶ┬␎ࡉࢀࡿ㸬ࡲࡓ㸪ᾐₕἲ࡛ࡢ᭷ᶵࢩࣛࣥ
ศᏊࡢ㔜ྜ࡟ࡼࡿ⢏Ꮚ≧ࡢሁ✚≀ࢆῶ㔞࡛ࡁࡿ฼Ⅼࢆࡶࡘ㸬⵨╔ἲࢆ฼⏝ࡋ㸪࢞ࣛࢫᇶᯈཬࡧ μAy ࡢ࢞ࣛࢫࢫ࣏ࢵࢺୖ࡬ࡢ㧗ᐦᗘ࡞࣏࣐࣮ࣜᅛᐃἲࢆ᳨ウࡋ㸪᪂つ㛤Ⓨࡋࡓྛ⵨╔ἲࢆᾐₕἲ࡜ẚ㍑ࢆ⾜࡞ࡗࡓ㸬స〇ࡋࡓ࢞ࣛࢫᇶᯈ࡛ࡢ࣏࣐࣮ࣜಟ㣭ࢆ☜ㄆࡍࡿ
ࡓࡵ࡟㸪SPM ᐃ࡜Ỉࡢ᥋ゐゅ ᐃࢆ⾜࡞ࡗࡓ㸬Ỉࡢ᥋ゐゅ ᐃࡼࡾᮍಟ㣭࢞ࣛࢫ࡛ࡣ ǉ=63°࡛࠶ࡗࡓ㸬P(macPEG-co-NAS)-g-glass㸪P(macPEG)-g-glass ࡛ࡢྛ APMSi ᅛᐃ໬ἲ࡟࠾ࡅࡿỈࡢ᥋ゐゅࢆ Table 1 ࡟♧ࡍ㸬APMSi ᅛᐃ໬ἲࢆኚ໬ࡉࡏࡓࡑࢀࡒࢀࡢ g-glass ࡣᮍฎ⌮࢞ࣛࢫ࡜ẚ࡭඲࡚ࡢᇶᯈ࡛⾲㠃ࡢ⃿ࢀᛶࡀኚ໬ࡋ
࡚࠸ࡓ㸬P(macPEG)࡜ P(macPEG-co-NAS)࡛ẚ㍑ࡍࡿ
࡜㸪P(macPEG) ࡀ࠸ࡎࢀࡶ᥋ゐゅࡀ㧗ࡃ㸪␯Ỉᛶ࡜࡞ࡿࡇ࡜ࡀ☜ㄆ࡛ࡁࡓ㸬ࡇࢀࡣ㸪 P(macPEG-co-NAS)࡟ẚ
࡭㸪NAS ࡢඹ㔜ྜ࡟ࡼࡗ࡚㸪ഃ㙐㛫ࡢ❧య㞀ᐖࡀపୗࡋ㸪ᇶᯈ⾲㠃ୖ࡟ᅛᐃ໬ࡉࢀ࡚࠸ࡿ PEG 㙐ࡢ㔞࣭ᐦᗘ➼ࡀ㧗ࡃ࡞ࡗࡓ࡜᥎ᐃࡋ࡚࠸ࡿ㸬⾲㠃㛤ጞ㔜ྜᚋࡢ࢞ࣛࢫ⾲㠃ࡢ poly(macPEG)࡜ poly(macPEG-co- NAS)ࡢಟ㣭ࢆ☜ㄆࡍࡿࡓࡵ㸪኱Ẽ㞺ᅖẼୗ࡛ࡢ⾲㠃࡛ࡢ SPM ᐃ(30μm×30 μm)ࡢ⤖ᯝࢆ Fig.3,4 ࡟♧ࡍ㸬ᮍฎ⌮࢞ࣛࢫ࡛ࡣ
พฝࡀ↓ࡃࢼࣀࢫࢣ࣮࡛ࣝᖹ⁥࡛࠶ࡿࡇ࡜࡟ᑐࡋ㸪

APMSi ᅛᐃ໬᪉ἲࢆኚ໬ࡋࡓ⤖ᯝ㸪ྞୗࡆἲ㸪㈞௜ࡅἲ㸪㟼⨨ἲ࡛ poly(macPEG)࡜ poly(macPEG-co- NAS)ࢆ
ࢢࣛࣇࢺ㔜ྜࡋࡓ g-glass ࡛ࡣ㸪ࡑࢀࡒࢀ㸪ࡇࢀࡽ࡟࣏࣐࣮ࣜ⏤᮶࡜⪃࠼ࡽࢀࡿࢼࣀࢫࢣ࣮࡛ࣝࡢࣈࣛࢩ≧ࡢพฝࡀ⾲㠃ୖ࡟ほᐹࡉࢀࡓ㸬⵨╔㸫ྞୗࡆἲ࡛ࡣ㸪P(macPEG)-g-glass ᇶᯈࡣ┦ᑐⓗ࡟⢓ᛶࡀప࠸㒊ศࡀከࡃ㸪ᇶᯈ⾲㠃࡟࣏࣐࣮ࣜᒙࡀᆒ୍࡛࡞࠸㸪ୗ᪉࠿ࡽࡢ APMSi ⵨Ẽࡀᇶᯈ࡟ᆒ୍࡛࡞࠿ࡗࡓࡓࡵ㸪࣏࣐࣮ࣜᅛᐃ໬ࡢ࣒ࣛࡀ⏕ࡌࡓ࡜⪃࠼ࡽࢀࡿ㸬ࡲࡓ㸪⵨╔㸫㈞௜ࡅἲ࡛ࡣ㸪P(macPEG-co-NAS)-g-glass㸪P(macPEG)-g-glass ඲࡚ࡢ macPEG 㙐ᅛᐃ໬ᇶᯈ࡟࠾࠸࡚㸪ᾐₕἲ࡛ࡢ࣏࣐࣮ࣜᅛᐃ໬ᇶᯈࡼࡾࡶ⢓ᙎᛶኚ໬㸪พฝࡀᑡ࡞ࡃ㸪ᾐₕἲ࡟ㄆࡵࡽࢀࡿ✵㝽ᵝࡢพฝࡶᑡ࡞࠸㸬⾲㠃඲యࢆᆒ୍࡟࣏࣐࣮ࣜᅛᐃࡀ࡛ࡁࡓ࡜⪃࠼ࡽࢀࡿ㸬ࡇࢀࡣ㸪APMSi ⵨Ẽࡀᆒ୍࡟࢞ࣛࢫᇶᯈ㠃࡟᥋ゐࡋ㸪
APMSi ࡢ SAM ⭷ࡀᙧᡂࡉࢀࡓࡓࡵ࡜᥎ᐃࡋ࡚࠸ࡿ㸬㓄ྥᛶࡢ SAM ⭷ᙧᡂ࡟ࡼࡗ࡚࣏࣐࣮ࣜ㔜ྜࡢ㊊ሙ࡜࡞ࡿAPMSi ࡢ࢔࣑ࣀᇶᮎ➃ࡀᇶᯈ⾲㠃࡟ᆒ୍࡟ᅛᐃ໬ࡉࢀࡓࡓࡵ㸪࣏࣐࣮ࣜᅛᐃ㔞ཬࡧ⾲㠃ࡢพฝཬࡧ⢓ᙎᛶീࡢᆒ୍ᛶࡀ㧗ࡲࡗࡓ࡜⪃࠼ࡽࢀࡿ㸬ࡉࡽ࡟㸪⵨╔㸫㟼⨨ἲ࡛ࡣ㸪๓㏙ࡋࡓ⵨╔ἲ 2 ἲ࡟ẚ࡭㸪ᇶᯈ࠿ࡽ APMSi ࡀ㞳ࢀ࡚㟼⨨ࡉࢀ㸪⾲㠃࡬ࡢᅛᐃ໬㔞ࡢῶᑡ࡟ࡼࡾ㸪࣏࣐࣮ࣜᅛᐃ㔞ࡀపୗࡋ㸪௚᪉࡜␗࡞ࡿ⾲㠃ᛶ≧࡟ኚ໬ࡋࡓ
࡜⪃࠼ࡿ㸬ᚑࡗ࡚㸪APMSi ⵨Ẽࡢᇶᯈ࡬ࡢᆒ୍࡞ᅛᐃ໬
ᇶᯈ࡜᥋ゐࡉࡏࡿࡓࡵ㸪ᇶᯈ࡜ APMSi ࡢ㓄⨨ཬࡧ
APMSi ⵨Ẽὶືᛶ➼ࡢ᭱㐺໬ࡀᚲせ࡛࠶ࡿ㸬Fig.3. ᾐ ₕ ἲ ࡜ ⵨ ╔ ἲ ࡛ స 〇 ࡋ ࡓ P(macPEG)g-glass ࡢ SPM ീFig.4. ᾐ ₕ ἲ ࡜ ⵨ ╔ ἲ ࡛ స 〇 ࡋ ࡓ P(macPEG-coNAS)-g-glass ࡢ SPM ീ－ 62 －Table 1. ␗࡞ࡿ APMSi ᅛᐃ໬ἲ࡟ࡼࡾㄪ〇ࡋࡓ
PEG 㙐ᅛᐃ໬࢞ࣛࢫ⾲㠃ࡢ᥋ゐゅ ⵨╔㈞௜ࡅἲࢆ⏝࠸ࡓ PDF3(* ࢢࣛࣇࢺ໬࢔ࣞ࢖>SRO\ PDF3(* Jw$\@ࡢ࢟ࣕࣛࢡࢱࣜࢮ࣮ࢩࣙࣥ 3.1 ⠇ࡢ⤖ᯝࡼࡾ㸪⵨╔ἲࢆ⏝࠸ࡓ APMSi ࡢᅛᐃ໬ἲࡣ⵨╔-㈞௜ࡅἲࡀᮏ◊✲࡛ࡣ᭱㐺࡛࠶ࡿ࡜ุ᩿ࡋࡓ㸬ࡑࡇ࡛㸪μAy ᇶᯈ࡬ࡢ APMSi ᅛᐃ໬ࢆ⵨╔-㈞௜ࡅἲ࡟ࡼࡾ⾜࠸㸪ᮏ◊✲ᐊ࡛ᚑ᮶౑⏝ࡋ࡚ࡁࡓᾐₕἲ࡜ẚ㍑᳨࣭
ウࡋࡓ㸬⵨╔-㈞௜ࡅἲ࡟ࡼࡾ㸪ᇶᯈ⾲㠃࡬ࡢ࢔࣑ࣀᇶᅛᐃ໬㔞ࢆቑࡸࡍࡇ࡜ࡀྍ⬟࡛㸪࣏࣐࣮ࣜᅛᐃ໬ᐦᗘࡸ⾲㠃ࡢᆒ୍ᛶࡀቑຍࡍࡿ࡜⪃࠼ࡽࢀࡿ㸬ᾐₕἲ࡜⵨╔-㈞௜
ࡅ࡟ࡼࡾస〇ࡋࡓ PEG ࡽ㙐ᅛᐃ໬ μAy ࡢỈࡢ᥋ゐゅᐃࡢ⤖ᯝ㸪ᮍฎ⌮ μAy ᇶᯈࡣ 75°࡛࠶ࡿࡢ࡟ᑐࡋ㸪ᾐₕἲ㸪⵨╔-㈞௜ࡅἲ࡛స〇ࡋࡓ g-μAy ࡣ࠸ࡎࢀࡶ 55r࡜⾲㠃ࡢ⃿ࢀᛶࡀྥୖࡋࡓ㸬ᾐₕἲ࡜⵨╔-㈞ࡾ௜ࡅἲ࡛᥋
ゐゅࡣ࡯ࡰྠࡌࡢ 55r࡛࠶ࡗࡓࡀ㸪ᾐₕἲཬࡧ⵨╔㈞௜
ࡅἲ࡛ㄪ〇ࡋࡓ μAy ࡢ SPM ᐃ࡟ࡼࡿ⾲㠃ゎᯒࢆ⾜ࡗࡓ⤖ᯝ㸪ᾐₕἲ࡛ㄪ〇ࡋࡓ μAy ࡢ࣏࣐࣮ࣜࣈࣛࢩࡣ㸪3.1⠇ࡢ SPM ᐃ࡜ྠᵝ࡟㸪⾲㠃ࡢพฝ࠿ࡽ㸪࣏࣐࣮ࣜࣈࣛࢩࡢᆒ୍ᛶపୗࡀ⪃࠼ࡽࢀ㸪⵨╔-㈞ࡾ௜ࡅἲ࡟ࡼࡾㄪ
〇ࡋࡓ μAy ࡣพฝࡀᑡ࡞ࡃᆒ୍࡞⾲㠃࡟࡜࡞ࡾ㸪᥋ゐゅ࡛ࡣุ᩿࡛ࡁ࡞࠿ࡗࡓࡀ PEG 㙐ࡀ㧗ᐦᗘࡋ࡚࠸ࡿ࡜⪃࠼ࡽࢀࡿࡀ㸪ヲ⣽ࡣ⌧ᅾ᳨ウ୰࡛࠶ࡿ㸬 ࡲࡓ㸪⵨╔㈞௜ࡅἲࡣᾐₕἲ࡛స〇ࡋࡓ μAy ࡼࡾࡶPEG 㙐ࢆ㧗ᐦᗘ࡟ᇶᯈ࡟ᅛᐃࡍࡿࡇ࡜ࡀྍ⬟࡛࠶ࡿ࡜⪃࠼ࡽࢀ㸪⣽⬊⼥ྜ࡟ࡼࡿ HAC ᑟධࡸ DNA㸪 siRNA㸪ࢱࣥࣃࢡ㉁➼ࡢᑟධ⏝⣲ᮦ࡜ࡋ࡚᭷⏝ᛶࢆ᳨ウࡋ࡚࠸ࡿ㸬 ࡲ࡜ࡵձSPM ᐃ࡜Ỉࡢ᥋ゐゅࡢ ᐃ⤖ᯝ࡟ࡼࡾ㸪ྛ⵨╔ἲࡢ࠸ࡎࢀࡶ࢞ࣛࢫ㠃࡬ࡢ PEG 㙐ࡢᅛᐃ໬ࢆㄆࡵࡓ㸬ղᾐₕἲ࡜⵨╔ἲ࡛స〇ࡋࡓ࢞ࣛࢫᇶᯈ࡛ࡣ㸪P(macPEG-co-NAS)ࡼࡾࡶ P(macPEG)ࡢ⃿ࢀᛶࡀቑຍࡋࡓ㸬ճ⵨╔ἲ࡛ㄪ〇ࡋࡓ࢞ࣛࢫཬࡧ μAy ࡣᾐₕἲ࡟ẚ࡭࡚⾲㠃ࡢ࣏࣐࣮ࣜࣈࣛࢩࡀᆒ୍໬ࡋࡓ㸬մྛ⵨╔ἲࢆ᳨ウࡋࡓ⤖ᯝ㸪⵨╔-㈞௜ࡅἲࡀ᭱㐺࡛࠶ࡿ࡜⪃࠼ࡿ㸬௨ୖࡢ⤖ᯝ࠿ࡽ㸪ᾐₕἲࡼࡾࡶ⵨╔ἲࢆ⏝࠸࡚స〇ࡍࡿᇶᯈࡀ APMSi ࡢᇶᯈ⾲㠃࡬ࡢ㓄ྥᅛᐃ໬࡟ࡼࡾ㸪࣏࣐࣮ࣜࣈࣛࢩࡀᆒ୍໬࡜㧗ᐦᗘ໬ࡀྍ⬟࡛࠶ࡿ࡜⪃࠼ࡽࢀࡿ㸬ᮏἲࢆ⏝࠸࡚స〇ࡍࡿ μAy ࡣᾐₕἲ࡛స〇ࡋࡓ μAy ࡼࡾࡶ⣽⬊ෆ࡬ࡢ㑇ఏᏊᑟධ⿵ຓ๣࡛࠶ࡿ PEG 㙐ࡢᐦᗘࡀቑࡋ㸪᭦࡞ࡿ㑇ఏᏊᑟධࡢຠ⋡໬ࡀᮇᚅࡉࢀࡿ㸬ㅰ㎡ᮏ◊✲ࡢ୍㒊ࡣ⤒῭⏘ᴗ┬ᆅᇦ࢖ࣀ࣮࣋ࢩࣙࣥ๰ฟ㛤Ⓨ஦ᴗ㸦+22 ᖺᗘ⤒῭⏘ᴗ┬㸧ཬࡧᡓ␎ⓗᇶ┙ᢏ⾡㧗ᗘ໬᥎㐍஦ᴗ +24 ᖺᗘ⤒῭⏘ᴗ┬ ࡢᨭ᥼࡟ࡼࡿࡶࡢ࡛࠶ࡾ㸪ࡇࡇ࡟ㅰពࢆ⾲ࡋࡲࡍ㸬ཧ⪃ᩥ⊩(1) S. Yamanaka et al, Cell, 126 (4), (2006), 663, 㧗ᶫᨻ௦㸪Ꮫ⾡ࡢືྥ㸪14(8), (2009), p8-14.
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