Functionality evaluation for Textile products: Antibacterial Test
【Test method for each type】
JIS L1902 Bacterial solution absorption method
This is the most common method among the antibacterial tests for textiles and is applied
to the testing method for SEK mark of Japan Textile Evaluation Technology Council.
Similar international method: ISO 20743, GB T 20499-2【Overview】
Inoculate and culture bacterial solution on a test specimen with antibacterial finishes anda control sample (cotton standard cloth) to be compared. After a certain period under thesame condition, the number of bacteria or the amount of ATP (intracellular), on the two
samples are measured and compared.

Measurement

Bacterial solution

Samples

Inoculation Bacterial solution

Wash out

1. Pour-plate culture (colony method)

2. Luminescence measurement (ATP method)

Visually measure the number of bacteria

Measure the number of bacteria by the lighton the colony formed on agar medium

intensity from chemical luminescence ofbacteria energy (ATP)Figure Image
Fungi detected on the scale (colony)
Left: Control sample (Unfinished)
Right: Test sample (Finished)

Figure Image
Image of luminescence【Evaluation / Standard value】Contact regarding
antbacterial test

Evaluate with antibacterial activity value [A].
Calculation formula of activity value

Japan
Biochemical Group（Biochemical Lab.）
Tel: +81-3-5875-7271

China
Shanghai Lab.
Tel:+86-21-6040-6388

Standard

Type of finishes

Standard value

JIS

Antibacterial finished[A]≧2.0

SEK

Antibacterial anddeodorant finished
Antimicrobial finishedfor general use
Antimicrobial finishedfor specific use

Antibacterial activity value [A](LogCt-LogC0)- (LogTt-LogT0)=F-G[A]≧2.2[A]≧[F][A]＞[F]

Number of living bacteria

Standard clothAntibacterial finishedcloth 1

Antibacterial funishedcloth 2
Immediately after inoculation

after 16 hours of cultivation

length of cultivation

A：Antibacterial activity value
F：Proliferation of fungi on control sample
G：Proliferation of fungi on finished test sample
LogCo：Common logarithm value of the average of 3 samples immediately after inoculationof the control sample
LogCt：Common logarithm value of the average of 3 samples immediately after cultivation of
the control sample
LogTo：Common logarithm value of the average of 3 samples immediately after inoculationof the test sample
LogTt：Common logarithm value of the average of 3 samples immediately after cultivation ofthe test sample【Type of bacteria used for tests according to SEK mark】

Antibacterial

Antimicrobial finished productfinished product

General use

Specific use

Type of bacteria

JISStaphylococcus aureus◎◎◎

Klebsiella pneumoniae

○○―

◎◎

MRSA

―――

◎Escherichia coli――▲▲

Pseudomonas aeruginosa ――▲▲

Moraxella osloensis――▲○：Can be specified optionally according to the usage of the product◎：Indispensable bacteria▲：Optional bacteria that can be specified as optional test▲

For “JEC301 SEK mark certification standard for textile products”, in order to evaluate thewashing durability of the finishes, the tests will also be conducted on specimens that havebeen subjected to washing process prescribed in the certification standard.

JIS Z 2801
This test is to measure the antimicrobial activity of non-porous surfaces, such as plastics
and metals etc. among products treated with antimicrobial products.
Applied to the testing of SIAA mark standard.
Similar Abroad Standard：ISO 18184【Overview】
1. Use a plate specimen and inoculate the test solution
onto the finished sample and unfinished sample.
2. Be sure that the bacterial solution contacts a certain area
evenly, then cover it with a film and oppress.
3. After a certain time of cultivation, the number of bacteriaon the sample is measured and compared.
4. The calculation of how much the growth of bacteria wassuppressed, is calculated as antibacterial activity value.
【Evaluation / Reference value】
Evaluate according to antibacterial activity value [R]Formula for activity valueStandard

Antibacterial activity value [R]JIS(Ut - Uo) – (At – Uo) = Ut – At

SIAA

Reference value[R]≧2.0

Viable countControl sample

Test sample

length of cultivation
Immediately after inoculation

after 24 hours of cultivation

R：Antibacterial activity value
Uo：Logarithmic value for the viable count of the unfinished test sample immediately after inoculation
Ut：Logarithmic value for the viable count of the unfinished test sample 24 hours after cultivation
At：Logarithmic value for the viable count of the antibacterial finished test sample after 24 hours【Antibacterial Test】

Head office2-16-11, Kuramae,
Taito-ku, Tokyo,
111-0051 JAPAN
Tel: +81-3-3861-2341
一般社団法人繊維評価技術協議会の、抗菌加工 SEK マーク
を詳しく知りたい方Bacteria

JIS regulation

SIAA standard

Staphylococcus aureus◎◎

Escherichia coli◎◎◎Indispensable bacteria
As we correspond to other non-standardized bacteria tests, please feel free to consult withus.