Support For in vitro ADME/Tox research
OYC laboratory research reagents

Intestinal Epithelial Differentiation
Media for Caco-2 Cells
Human colon cancer-derived cells (Caco-2) are known todifferentiate into intestinal epithelial-like cells by
overcrowding culture, and are widely used in drug discovery
research fields as a cell model that reproduces intestinal
function in the small intestine.
This product is a serum-free medium for intestinal
epithelial differentiation of Caco-2 cells. By utilizing thisproduct, it is possible to shorten the differentiation period,
which generally takes about 3 weeks, to about 5 days.

Product Specifications
Intestinal Epithelial
Differentiation Media for Caco-2 Cells
LiquidFree
250 mL per bottle
Refrigerated temperatures (1-10℃)＜Application＞

TEER of Caco-2 cell monolayer
during intestinal epithelial differentiation process

TEER (Ω・cm2)

https://www.oyc.co.jp/bio

Form:Serum:
Volume:Storage:

Culture period from cell seeding (days)
Caco-2 cells were seeded in 24-well plate culture inserts with 2x105 cells(day 0) and replaced with each intestinal epithelial differentiation medium
the next day (day 1). Then, the medium was exchanged with the intestinal
epithelial differentiation medium every 1 to 2 days, and TEER
(transepithelial electrical resistance) of monolayer was measured.

Support For in vitro ADME/Tox research
OYC laboratory research reagents
A test for intestinal absorption
A test for intestinal absorption was conducted using the intestinal epithelial
differentiated by this product, and absorption was evaluated by HPLC analysis of theB side medium. The permeability coefficient was calculated by the following formula.

Permeability coefficient (cm/sec) =CompoundA

IncubationMediumchange

Cell seeding

dQ/dt：Permeability rate
A：Area (cm2)
Co：Initial concentration (μg/mL)

dQ/dt
AxC0

B

TEER Measurement

Addition of the sample

Incubation

HPLC analysis andaddition of Lucifer yellow

Lucifer yellow assay

https://www.oyc.co.jp/bio

Medicine

Applications

Permeability
coefficient(cm/sec)

Permeability
evaluation by
FDA

Propranolol

Treatment of arrhythmia,
hypertension, myocardial
infarction,glaucoma and migraine

8.7 x 10-6High

Ketoprofen

Transdermal analgesic and antiinflammatory drug

5.6 x 10-6

High

Atenolol

Treatment of angina pectoris

3.0 x10-7Low※The expression of transporters (P-gp, BCRP) has been confirmed by RT-PCR
on the monolayer membrane formed by this product.
※It has been confirmed that Lucifer Yellow does not permeate the monolayer membrane
formed by this product.
Product name
Intestinal Epithelial
Differentiation Media for Caco-2 Cells

OYC No.

Package47338000

250 mL＊For bulk inquiry, please contact our sales representative or visit the following website.
＊For research purposes only. Not for therapeutic or diagnostic use.

Reference cited this product
Reference・ Evidence-Based Complementary and AlternativeMedicine 2022 Sep, Article ID 4139812, 9 pages.
・ J Basic Clin Pharm. 2010 Mar;1(2):63-9.
・ Br J Pharmacol. 2012 Apr;165(8):2598-610.

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2023.03