HepExtend™ Supplement (50X)
Description
HepExtend™ Supplement (50X) is an enriched supplement designed to enhance the culture lifespan of cryopreserved primary
hepatocytes. Use the supplement in conjunction with Williams’ Medium E (Cat. no. A12176) and the Hepatocyte MaintenanceSupplement Pack (Cat. no. CM4000) following the recommended hepatocyte culture methods. When cultured in the presence of theHepExtend™ Supplement, primary human hepatocyte lots will live and be functional for 10 days or longer depending on the lot of cells
and other assay conditions. HepExtend™ supplement does not contain growth factors, cytokines, fetal bovine serum, or pharmaceuticalsmall molecules.
Product*HepExtend Supplement (50X)™

Catalog no.

AmountStorage

Shelf life*A27375-01

10 mL–20°C to –5°C; Protect from Light

12 months* Shelf life duration is determined from Date of Manufacture.

Product use
For Research Use Only. Not for use in diagnostic procedures.

Safety information
Read the Safety Data Sheets (SDSs) and follow the handling
instructions. Wear appropriate protective eyewear, clothing, andgloves.

Important information™

Divide thawed HepExtend Supplement (50X) into aliquots andstore in a non-frost-free freezer at –20ºC to –5ºC. Do not freezethaw HepExtend™ Supplement (50X) more than twice.

Prepare medium™

1. Quickly thaw HepExtend Supplement (50X) in a 37°C
waterbath (should thaw in under 10 minutes). Do not
prolong exposure of the concentrated supplement to 37°C.
2. Prepare Hepatocyte Maintenance Medium by adding
Hepatocyte Maintenance Supplement Pack (Cat. no.CM4000)
to Williams E Medium (Cat. no. A12176). For further details
on preparing hepatocyte media, refer to Gibco® hepatocyte
media supplement guide.
3. Add HepExtend™ Supplement (50X) to the Hepatocyte
Maintenance Medium to a final dilution of 1X. For example,
add 10 mL of HepExtend™ Supplement (50X) to 500 mL ofWilliams’ E Medium for a 1X complete medium.

Related products
ProductCat. no.

Williams' Medium E (1X) without L-glutamine,
without phenol red

A12176

Primary Hepatocyte Maintenance Supplements

CM4000Primary Hepatocyte Thawing and Plating
Supplements

CM3000Geltrex® LDEV-Free Reduced Growth Factor
Basement Membrane Matrix

A14132

Collagen I, Coated Plate

A11428Hepatocyte Thaw Medium

CM7500

Human Plateable Hepatocytes, Transporter Qualified

HMCPTS

Human Plateable Hepatocytes, Induction Qualified

HMCPIS

Human Plateable Hepatocytes, Metabolism Qualified

HMCPMS

Explanation of symbols and warnings
Caution, consult
accompanying
documents

Temperature
Limitation

Keep away fromlight

Use By:Consult
instructions
for useBatch CodeCatalognumberManufacturer

Sterilized using
aseptic processing
techniques

Read Safety
Data Sheet

4. Optional: Filter complete medium using 0.22-μm filter units.
5. Once supplemented, the complete medium is stable for up to3 weeks when stored at 2–8°C in the dark.
6. Warm complete medium in a 37°C waterbath prior to daily
feeding of cells. We recommend aliquotting the medium andwarming only what is needed for the daily feed, keeping themain batch of medium at 2–8°C.
7.

Refer to Gibco® Thawing and Plating Cryopreserved
Hepatocytes protocol for detailed information on plating andculturing primary cryopreserved hepatocytes.

Note: HepExtend™ Supplement (50X) contains 0.12375 g/mL
bovine serum albumin (BSA). Hepatocyte MaintenanceSupplement (Cat. no. CM4000) contains 0.125 g/mL BSA, prior todilution. The final complete HepExtend™ Hepatocyte
Maintenance Medium will contain 0.003725 g/mL BSA.

Publication Number MAN0013760

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This product may be covered by one or more Limited Use Label
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Revision 1.0

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