Anti-Rad51 antibody, rabbit polyclonal, ChIp grade
Product code70-012Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityAffinity-purified with immunogen.

Immunogen

Full-size recombinant human Rad51 protein (BioAcademia #10-001)

Isotype

N/A

Reactivity

Human, Rodents, Chicken, Xenopus

Validation

N/A

Application

1. Western blotting (1/1,000 ~1/10,000 dilution). Fig.1
2. Immunoprecipitation(1/1,00-1/1,000). Fig.2
3. Chromatin Immunoprecipitation (ChIp) (assay dependent)
4. Immuno-fluorescent staining (1/1,000~1/10,000). Fig.3
5. Immunohistochemistry (1/100-1/1,000). Fig.4
6. Dot blotting (1/1,000~1/5,000 dilution)
7. ELISA (indirect, 1/2,000~1/5,000 dilution)
Not tested for other applications

Background

Human Rad51 protein is a functional and structural homolog of E.coli RecA
protein, which plays a major role in genetic recombination and recombination
repair by mediating strand exchange reaction between homologous DNA
strands. Rad51 functionally and physically interacts with its paralogs Dmc1,Rad51B, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination
processes.

It also interacts with oncogenes and tumor suppressors such as

BRACA1, BRACA2, and p53 for the maintenance of genome stability.

Data Link

UniProtKB Q06609 (RAD51_HUMAN), UniProtKB Q08297 (RAD51_MOUSE)
UniProtKBB5DF04 (B5DF04_RAT),
UniProtKBA0A1L8FB28 (A0A1L8FB28_XENLA)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 70-012 Anti-Rad51 antibody, rabbit polyclonal

Fig.1 Western blotting of endogenous Rad51 proteins in crude extracts of varius animal cells.
15% SDS-PAGE was used. Blotting was done at 15 v overnight with wet system. The antibody wasused at 1/1000 dilution.
1. MCF7 (human) cells. 40 µg
2. NIH3T3 (mouse) cells. 40 µg
3. CHO (hamster) cells. 40 µg
4, Xenopus laevis (frog) egg. 40µg

Fig.2. Western blot of endogenous Rad51 protein in rat cell lysate.
Proteins in the lysates were separated on SDS-PAGE (12.5 % gel) and blotted to PVDF membrane at
15 V overnight. The membrane was blocked with 3% skim milk. The anti-Rad51 antibody was used
at 1/1000 dilution and as the second antibody, goat anti-rabbit IgG (ab97051) was used at 1/10000
dilution.
1. Human MCF7 cell lysate (80 µg)
2. Rat PC12 cell lysate (80 µg)

2 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Fig.3. Immunoprecipitation of Rad51 protein from crude extract of HeLa cells by the anti-Rad51
antibody.
Anti-Rad51 antibody (20 µg) was incubated with 20µg of HeLa cell extract, and precipitated with 20µg of proteinA-beads. The sample was dissociated from the precipitate by heating in SDS-sample
buffer and analyzed by western blotting with anti-Rad51 antiserum (chicken, ab63802) at 1/1000
dilution. As second antibody, anti-chicken IgG antibody (rabbit) was used at 1/10000 dilution.

Fig.4. Detection of Rad51 foci formation after X-ray irradiation in human fibroblast cell line, GM0637.
Cells were irradiated by X-rays at 2 Gy,, grown for 1 hr, fixed with 4% paraformaldehyde in 1x PBSfor 10 min, washed 3 times with PBS for 3 min, permealized by treatment with 0.5% Triton for 5 min,3 / 4BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/washed 3 times with PBS for 3 min, incubated with anti-Rad51 antibody for 30 min at 37℃, washed
3 times with PBS for 3 min, incubated with secondary antibody for 30 min at 37℃, washed 3 times
with PBS for 3 min, stained with Hoechst for 1 min and mounted.
Anti-Rad51 antibody was used at 1/10,000 dilution (left panels) and 1/1,000 dilution (right panels).
As the secondary antibody, anti-rabbit Alexa 488 was used at 1/10,000 dilution. The pictures were bycourtesy of Prof. S. Tashiro and Dr. K. Kono at Hiroshima University.

Fig.5. Immunohistological staining of Rad51 protein in mouse testis using anti-Rad51 antibody.
A section of formalin fixed and paraffin embedded mouse testis was treated with the anti-Rad51
antibody at 1/100 dilution after deparaffization and antigen retrieval. The 2nd antibody, anti-rabbit
IgG conjugated with Alexa Fluor 647 (Abcam) was used at 1/1,000 dilution. DNA was stained withDAPI and the merged image was shown (Merged). The plain black and white microscopic picture of
the same region was shown on the right.
Related Products :10-001 Rad51 protein (human)
10-003 Rad52 protein (human)
70-001 Anti-Rad51 antibody, rabbit serum
70-005 Anti-Rad51 (human) antibody, IgY
70-015 Anti-Rad52 antibody, rabbit polyclonal
References: Please let us know any publications using this antibody.

4 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/