Anti-IGSF8 antibody, rabbit polyclonal
Product code73-038

Size

100 µlStorage-20℃

Concentration

0.5 mg/ml

Buffer

PBS- with 50% glycerol, 0.05 % sodium azide

PurityPurified IgG fraction with protein A from rabbit antiserum.

Immunogen

Full-length mouse IGSF8 with Flag tag

IsotypeRabbit IgG

Reactivity

Mouse.
Likely to react with rat and human due to high sequence homology.

Special notes

Validation: Specificity validated with knock-out mouse (Fig.1)

Application

1. Western blotting (1/500~1/1,000 dilution))
2. Immunofluorescence and immunochemical staining (1/100 dilution).
3. Immunohistochemical staining (1/100)

Background

IGSF8 may play a key role in diverse functions ascribed to CD81 and CD9 suchas oocytes fertilization or hepatitis C virus function. May regulate proliferationand differentiation of keratinocytes. May be a negative regulator of cell motility:
suppresses T-cell mobility coordinately with CD81, associates with CD82 tosuppress prostate cancer cell migration, regulates epidermoid cell reaggregationand motility on laminin-5 with CD9 and CD81 as key linkers. May also play arole on integrin-dependent morphology and motility functions. May participate
in the regulation of neurite outgrowth and maintenance of the neural networkin the adult brain.
Molecular mass: 65,011 Da with 611 amino acids

Data Link

uniprot/Q8R366 mouse IGSF8

Gene ID140559 mouse IGSF8

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 73-038 Anti-IGSF8 antibody, rabbit polyclonal

Fig 1. Analysis of IGSF8 protein in various tissues of Igff8-targeted mice by western blotting with
anti-IGFS8 antibody.
Lysates of tissues (30 μg) were analyzed by western blotting using the antibody at 1/500 dilution. “ F ”
and “ – “ stand for floxed and knock-out alleles, respectively.

Fig.2

Detection of endogenous level of IGSF8 in crude extract of NIH3T3 cells by using anti-IGSF1

antibody.
Protiens in 40 µg of the cell extract were separated by 12.5% SDSD-PAGE and electro-blotted at 15v,
over night (wet system).
Blocking , 1hr, room temp.
1st antibody 1/1000 dilution
2nd, Goat polyclonal secondary antibody to rabbit IgG-H&L (HRP), ab97051
Positions marker proteins are shown in kDa on the left

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Fig.3

Immunofluorescence staining of IGSF8 protein in eggs of wild-type mouse and Igsf8 knock-out mouse with anti-IGSF8 antibody.
Zona-free eggs were fixed in PBS containing 0.5% (v/v) polyvinylpyrrolidone and 4% (v/v)
paraformaldehyde. The anti-IGSF8 antibody was used at 1/100
dilution and as the second antibody, Alexa-Fuor 546 labeled anti-rabbit IgG was used (red). Then theDNA was stained with Hoechst 33342 (blue). “DIC” is picture of Differential Interferernce Contrast
microscopy.

Fig.4. Immunofluorescence staining of IGSF8 protein in NIH3T3 cells with anti-IGSF8 antibody.
NIH3T3 cell were fixed in 4% (v/v) paraformaldehyde. The anti-IGSF8 antibody was used at 1/100
dilution and as the second antibody, Alexa-Fluor 488 labeled anti-rabbit IgG was used (green) at
1/1,000 dilution. DNA was stained with DAPI (blue).

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Fig.5 Immunohistochemical staining of IGSF8 protein in mouse lung tissue section using anti-IGSF1
antibody.
4％ PFA fixed section of mouse lung tissue
Deparaffinization ; LemosolRA (#122-03991,Wako, Osaka)
Rehydration
Antigen retrieval; Histo/Zyme (Cat.# k046; Diagnostic BioSystems)
Washing; PBST (0.25% triton X-100/PBS-)
Blocking; 1 % BSA / PBST60 min

1st antibody; 1/100 dilution in PBS- 4℃ overnight
Washing; PBS2nd antibody (Alexa-Fluor 488 labeled anti-rabbit IgG (green)) ; 1/1,000 dilution, 60 min
Washing; PBS-, 5 min 3 times
DAPI; 1.0 μg/mL DAPI in TBS

10 minWashing; PBSMount; ImmunoSelect Antifading Mounting Medium (SCR-38447; Dianova)
Reference: This antibody was described and used in the following publication.
1. Inoue N. et al Tetraspanin-interacting protein IGSF8 is dispensable for mouse fertility. Fertil
Steril. 2012 98(2):465-70.

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