Anti-Collectin-11 / CL-K1 antibody, mouse monoclonal (821)
Product code72-040Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

Recombinant human collectin-11 (amino acids 107 to 271) corresponding to thecarbohydrate recognition domain (CRD) expressed in Escherichia coli.

Isotypemouse IgG1κ

Reactivity

Human, mouse, rat and chinese hamster

Special notes

N/AApplication

1. Western blot(1/500~1/1,000 )2. Immunofluorescent staining (1/500)
3. ELISA (assay dependent)

Background

Collectins are characterized by a collagen-like sequence and a carbohydrate
recognition domain (CRD) and are members of the vertebrate C-type lectinsuperfamily. Collectins play an important role in the innate immune system.
Collectin-11 (CL-K1) is a secreted-type protein and detected in human serum
and the Golgi of almost all organs. At least 10 isoforms produced by alternative
splicing are known. Isoform 1 of Collctin-11 has an open reading frame of 813
base pairs encoding 271 amino acids. It has a signal peptide consisting of 25
amino acids at N-terminal. CL-K1 in supernatant of CL-K1-cDNA-tranfected
CHO cells shows an apparent molecular mass of 34 kDa

Data Link

UniProtKB Q9BWP8 (COL11_HUMAN) GenBank accession number : 78989,AB119525, AB119650, AB119651 and AB119652.

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Fig.1. Identification of Collectin-11 protein by Western blotting
Supernatant of CHO cells expressing human recombinant Collectin-11 (1µg/ml) was separated on
SDS-PAGE. The monoclonal antibody was used at 1/500 dilution.
The collectin protein migrates at 34 kDa position

Fig.2. Detection of collectin-11 protein transfected in CHO cells by immunofluorescence staining
A: CHO cells expressing human recombinant Collectin-11.
B: CHO cells (mock infected cells).
The antibody was used at 1/500 dilution.

The FITC-conjugate rabbit anti-mouse IgG (x4000) was added.

Nucleus (DNA) was stained with DAPI.

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50 2x102 8x102 3x103 1x104 5x104 2x105
Concentration (pg/ml)

Fig.3 Titration of recombinant CL-K1 protein transfected in CHO cells by indirect ELISA using monoclonal
antibody.
The indicated amounts of recombinantCollectin-11 protein expressed in CHO cells ware coated onto thewells of the ELISA plate. After blocking with 5% skim milk, monoclonal antibody at the 1/1000 dilution
was added to the each well. HRP-conjugated goat anti-mouse IgG (100μl, x4000 dilution) was added. As
substrate, orthophenylenediamine was used. Optical density (OD) measured at 490nm.

Related product72-041 Anti-collectin placenta 1 (CL-P1), mouse monoclonal (53)References: This antibody was used and cited in the following publication.
Yoshizaki T et al. Comparison of human blood concentrations of collectin kidney 1 and mannanbinding lectin. J Biochem. 2012 Jan;151(1):57-64. PMID: 21893516. WB (human)

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