Lighting the way
with diagnostics

Fast and reliable spoiler monitoring

Flow VIT Alicyclobacillus

Flow into the next levelof quality control

www.ap.sysmex-flowcytometry.com

Unpleasant taste or smell?
Alicyclobacilli are frequently occurring beverage spoilage microorganismsthat can cause off-odours or flavours in juice or juice-containing beverages.
As their spores are resistant to normal pasteurisation methods, it can be achallenge to remedy the situation once they are present in a production lot and the resulting economic impact can be very high.

Flow VIT helps
to recognise
and prevent
Alicyclobacillus
contaminations.

Assurance of quality in beverage production– with Flow VIT Alicyclobacillus

The Flow VIT solution is a highly specific and automated detection system that enables fast and reliable quality
control testing for Alicyclobacilli. It allows you to detect beverage spoilers faster and with less effort than classicalculture methods, while at the same time avoiding false-positive results.
Flow VIT Alicyclobacillus detects all members of the genus Alicyclobacillus and, in parallel, specifically detects thedreaded species Alicyclobacillus acidoterrestris.

The Flow VIT Solution

CyFlow Cube 6with Flow VIT test kit

Flow VIT– the next level of microbiological analysis
The Flow VIT solution is the perfect combination of VIT gene probe technology and flow cytometry. Using the Flow
VIT solution, microbiological quality control is elevated to a higher level; paving the way for fast and cost-effective
microbiological monitoring of production processes.

Partnering to deliver new microbiologicaltesting solutions
The Flow VIT solution has been developed in a collaboration between Vermicon AG, known for their expertise in industrial
microbiology applications; Sysmex Europe GmbH and Sysmex Partec GmbH, pioneers in flow cytometry measurement
technologies. Through this partnership we aim to create more effective and economical solutions for industrial microbiology
testing.

Sysmex order codes
Reagent

Sysmex order code

Number of tests

Suitable flow cytometer system

Flow VIT Alicyclobacillus

01150001

50

CyFlow Cube 6 (CY-S-3060R__V2m)

VIT = vermicon identification technology

FLOW VIT

Fast & easy workflow
The workflow is fast and easy and allows highly specific detection of living
microorganisms in a short time, automatic evaluation included!

12aEasy sample treatment
and application of thegene probes

bVIT gene probes penetrate the cells andbind specifically to their target sites

34

Cell Sample

SheathFluidLaser

Detector

CyFlow Cube flow cytometer
analyses the samples

Automatic evaluation, reports
positive / negative results

Final evaluation within

3 minutes!

CyFlow Cube 6 and Flow VIT
Alicyclobacillus test kit
AssuranceCyFlowof quality
inbeverage
CyFlow
CubeCube6 &production
6&®
®
• Parallel detectionof Alicyclobacillus
spp. and A. acidoterrestris
FlowFlow
VIT
VIT
Alicyclobacillus
Alicyclobacillus
test testkit kit• Enumerate only living alicyclobacilli
Assuranceof qualityof quality
in beveragein beverage
production
production• Low limitAssuranceof detection• Easy interpretation of the results■ Parallel■ Parallel
detection
detectionof Alicyclobacillusof Alicyclobacillus
spp. spp.

• Reliable results
even on challenging fruit juices and concentrates
and A.andacidoterrestris
A. acidoterrestris• Fast and easy
workflow■ Enumerate■ Enumerate
only living
only alicyclobacilli
living alicyclobacilli■ Low
Lowoflimit
detectionofQualification
detection (IQ/OQ) protocols• Installation
and■limit
Operational■ Easy■interpretation
Easy interpretationof the ofresults
the results■ Reliable■ Reliable
resultsresults
even oneven
challenging
on challenging
fruit juices
fruit juices
and concentrates
and concentrates■ Fast■andFast
easy
andworkfl
easy workfl
ow ow

CyFlow Cube
CyFlow
6■ Installation■ Installation
and Operationaland Operational
QualifiQualifi
cationcation(IQ/OQ)(IQ/OQ)
protocolsprotocols
CyFlow Cube 6Analysis example using the Flow VIT Alicyclobacillus test kit®
AnalysisAnalysisexampleexampleusing using
the Flow
theVIT
Flow
Alicyclobacillus
VIT® Alicyclobacillus
test kit
test kit

Alicyclobacillus
Alicyclobacillus
spp.: spp.:
positive
positive
result result
Alicyclobacillus spp.:
positive result

Alicyclobacillus
Alicyclobacillus
acidoterrestris:
acidoterrestris:
negative
negative
result result
Alicyclobacillus acidoterrestris:
negative result

SysmexSysmexorder ordercodes codes
ReagentReagent

SysmexSysmex
order orderNumberNumber
ofof
SuitableSuitableflow cytometer
flow cytometersystem systemcode code
tests tests®
Alicyclobacillus
01150001
Flow VITFlow
VIT® Alicyclobacillus 01150001

50■50CyFlow■ CyFlow
Cube 6 (CY-S-3060R__V2m)
Cube 6 (CY-S-3060R__V2m)

VIT® = vermicon
VIT® = identifi
vermiconcationidentifi
technology
cation technology

VIT® is a registered
VIT® is a registeredtrademarktrademarkof vermiconof AGvermicon· www.vermicon.com
AG · www.vermicon.com

Authorised
Authorised
representative:
representative:
Sysmex EuropeSysmexGmbHEurope GmbH

BornbarchBornbarch
1, 22848 Norderstedt,
1, 22848 Norderstedt,
Germany ·Germany
Phone +49· Phone
40 52726-0+49 40· Fax
52726-0+49 40· Fax
52726-100+49 40 52726-100· info@sysmex-europe.com· info@sysmex-europe.com· www.sysmex-europe.com· www.sysmex-europe.com

Manufacturer
Manufacturerof CyFlowofCube
CyFlow
6: Sysmex
Cube 6:PartecSysmex
GmbHPartec GmbH

Design and specifications may be subject to change due to further product development.
Sysmex Asia Pacific Pte Ltd

Sysmex New Zealand LtdSysmex Vietnam Co., Ltd

Sysmex India Pvt. Ltd

Sysmex (Thailand) Co., Ltd

Sysmex Vietnam Co., Ltd (Hanoi Branch)

PT Sysmex Indonesia

Sysmex Philippines Inc.

Sysmex Australia Pty Ltd

Tel +65 6221-3629 Fax +65 6221-3687
www.sysmex-ap.com

Tel +64 (9) 630-3554
www.sysmex.co.nz

Tel +91 (22) 6112-6666
www.sysmex.co.in

Tel +66 (2) 032-2536 Fax +66 (2) 116-5396
www.sysmex.co.thTel +62 (21) 3002-6688 Fax +62 (21) 3002-6699
www.sysmex.co.id

Tel +63 (2) 621-2460 Fax +63 (2) 621-2432
www.sysmex.com.ph

Tel +84 (8) 3997-9400 Fax +84 (8) 3997-9405
www.sysmex.com.vn
Tel +84 (4) 3776-7020 Fax +84 (4) 3776-7022
www.sysmex.com.vn
Tel +61 (2) 9016-3040
www.sysmex.com.au

Sysmex (Malaysia) Sdn Bhd

Tel +60 (3) 5637-1788 Fax +60 (3) 5637-1688www.sysmex.com.myAuthorised Distributor in Asia Pacific Region

Lighting the way
with diagnostics
SAP/FLOWCYTOMETRY/FLOWVIT/09042019/V1.0