Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA1) (FITC)
Product code72-004

Size

50 µg

Storage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

ImmunogenPurified recombinant extracellular portion of human FcεR1α (corresponding toamino acids Met-26-197, where signal peptide is 1-25)

Isotype

Mouse IgG 2bReactivity

Human, house musk shrew

Special notes

Epitope: 26-110 amino acids
Conjugation: FITC ([FITC]/[IgG] = 5.0; depending on Lot)

Application

1. Western blotting
2. Immunofluorescence staining (2-10 µg/ml)
3. Flow Cytometry (FC) (2-10 µg/ml)
4. IHC-P, IHC-F (2-10 µg/ml)
5. Titration of IgE-bound receptor in combination with CRA2 antibody (Ref.3)

BackgroundFcεR1α is subunit of the high affinity receptor for IgE to which IgE directlybinds. FcεR1α is a tetrameric complex consisting of one α, one β and two γ
subunits. The latter two are required for signal transduction activity. The
FcεR1α complex plays an important role in triggering allergic responses.
The CRA1 (AER37) monoclonal antibody reacts with the FcεR1α subunit on aregion that does not overlap the region of the IgE binding site, thus it does notcompete with IgE for the receptor binding. Since the CRA2 (AER24) monoclonal
antibody reacts with the IgE binding site on FcεR1α, it competes with IgE forthe receptor binding. Combining the two antibodies, one can quantitatively
measure the amounts of the IgE-bound FcεR1α.

Data Link

UniProtKB/Swiss-Prot P12319 (FCERA_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 72-004 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA1) (FITC)

Fig.1 Levels of binding of CRA1 antibody measured via basophilic staining with (dashed line) orwithout lactic acid treatment (solid line) by flow-cytometrical analysis.
The levels of CRA1, on basophils in the patient 1 (grade 3 allergy) are shown in (A and B)(Images and data are from Iwamoto T et al Cancer Med. 2016 Jun;5(6):1004-12.)
References: This product has been used in the following publications.
1. Suzuki K. et al. The Fc receptor (FcR) γ subunit is essential for IgE-binding activity of cell-surface
expressed chimeric receptor molecules constructed from human high-affinity IgE receptor(FcεRI) αand FcRγ subunits. Mol Immunol. 1998 Apr;35(5):259-70. FC (human)
2. Wang X. et al. Optimisation and use of humanised RBL NF-AT-GFP and NF-AT-DsRed reporter
cell lines suitable for high-throughput scale detection of allergic sensitisation in array format andidentification of the ECM-integrin interaction as critical factor. Mol Biotechnol. 2014
Feb;56(2):136-46. PMID: 23893250 FC (human)
3. Iwamoto T et al. A novel approach to predict cetuximab-induced hypersensitivity reaction:
detection of drug-specific IgE on basophils. Cancer Med. 2016 Jun;5(6):1004-12. PMID: 26880699
FC (human)
Related product72-001 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA1)
72-003 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA1) (biotin)
72-005 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA2)
72-007 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA2) (biotin)
72-008 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA2) (FITC)

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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/