Anti-FcR1（human IgE receptor）antibody, mouse monoclonal (CRA2) (FITC)
Product code72-008Size50 µg

Storage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium

Immunogen

Recombinant extracellular portion of human FcεR1α (corresponding to aminoacids Met-26-197, where signal peptide is 1-25)

Isotype

Mouse IgG1κ

Reactivity

HumanSpecial notes

Epitope: Amino acids 110-197 of FcεR1α (Ref 3)
Conjugation: FITCApplication

1. Western blotting (～1 µg/ml)2. Flow-Cytometry
3. Immunohistochemistry (Paraffin and Frozen) and immunocytochemistry

BackgroundFcεR1α is subunit of the high affinity receptor for IgE to which IgE directly binds.
FcεR1 is a tetrameric complex consisting of one α, one β and two γ subunits.

The

latter two subunits are required for signal transduction activity. The FcεR1αcomplex
plays an important role in triggering allergic responses.
The CRA2 (AER24) monoclonal antibody reacts with the FcεR1αsubunit on a region
that overlaps the region of the IgE binding site, thus it competes with IgE for thereceptor binding.

Since the CRA1 (AER37) monoclonal antibody reacts with thesite different from the IgE binding site on FcεR1α, it does not compete with IgE forthe receptor binding. Combining the two antibodies, one can quantitatively measure
the amounts of the IgE-bound FcεR1α.

Data Link

UniProtKB P12319 (FCERA_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 72-008 Anti-FcR1（human IgE receptor）antibody, mouse monoclonal (CRA2)
FITC-labeled

Figure. Levels of binding of CRA2 antibody measured via basophil staining with or without lactic acidtreatment by ﬂow cytometric analysis.
Dashed and solid lines mean with and without lactic acid treatment, respectively. The levels of CRA2
on basophils in the patient 1 (grade 3 allergy) are in A.
(Images and data are from Iwamoto T et al Cancer Med. 2016 Jun;5(6):1004-12.)
Related product:72-001 Anti- FcR1（human IgE receptor）monoclonal antibody (CRA1)
72-003 Anti- FcR1（human IgE receptor）monoclonal antibody (CRA1), biotinylated
72-004 Anti- FcR1（human IgE receptor）monoclonal (CRA1), FITC conjugated72-005 Anti- FcR1（human IgE receptor）monoclonal (CRA2)
72-007 Anti- FcR1（human IgE receptor）monoclonal (CRA2), biotinylated
References: This product has been used in the following publications.
1. Suzuki K. et al. The Fc receptor (FcR) γ subunit is essential for IgE-binding activity of cell-surface
expressed chimeric receptor molecules constructed from human high-affinity IgE receptor(FcεRI) αand FcRγ subunits. Mol Immunol. 1998 Apr;35(5):259-70. FC (human)
2. Iwamoto T et al. A novel approach to predict cetuximab-induced hypersensitivity reaction:
detection of drug-specific IgE on basophils. Cancer Med. 2016 Jun;5(6):1004-12. PMID: 26880699
FC (human)
3. Perez‐Witzke D. et al. CTLA4Fcε, a novel soluble fusion protein that binds B7 molecules and theIgE receptors, and reduces human in vitro soluble CD23 production and lymphocyte proliferation
Immunology 2016, 148 (1), 40-55. PMID: 26801967 FC (human)

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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/