Anti-Nestin antibody, rabbit polyclonal (ST1)
Product code73-105Size

100 µgStorage-20℃

Concentration

2.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum.

ImmunogenSynthetic peptide corresponding to the C-terminal 15 aa of mouse nestin.

IsotypeRabbit IgG

Reactivity

Mouse and rat Nestin, but not with human Nestin.

Special notes

N/A

Application

1. Western blotting (dilution: 1/500-1/5,000)
2. Immunocytochemistry (dilution: 1/300-1/1,000)
3. Immunohistochemistry (dilution: 1/500-1/1,000)

Background

Nestin is a class VI intermediate filament protein that is abundantly expressedin stem cells and progenitor cells in the mammalian central nervous system(CNS) during development. Upon differentiation, nestin becomes downregulated and is replaced by other intermediate filament proteins. Nestin
expression is widely used as a marker for CNS stem cells in the developing
nervous system. Its transient expression is a critical step in the neuraldifferentiation pathway. Down-regulated nestin may be re-expressed in theadult organism under certain pathological conditions such as brain injury,
ischemia, inflammation, and neoplastic transformation.
This antibody (named ST1) against mouse nestin was raised by Prof. K.
Yoshikawa of Osaka University.

Data Link

UniProtKB Q6P5H2 (mouse), P21263 (rat)Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 73-105 Anti-Nestin antibody, rabbit polyclonal (ST1)

Fig.1 Detection of Nestin in whole extracts of mouse neocortex and neural stem cells by western
blotting with anti-Nestin antibody.
Lane 1; Mouse neucortex at E14.5
Lane 2; Neural stem cells in culture prepared from E14.5 mouse neocortex.
Lane 3; Neurons in culture differentiated from neural stem cells.
Nestin is detected only in undifferentiated neural stem cells
Anti-Nestin antibody (ST1) was used at 1/500 dilution. As 2nd antibody, HRP-conjugated polyclonalanti-rabbit IgG antibody was used at 1/20,000. As a loading control, western blot of γ-tubulin wasemployd.(* Stars indicate non-specific bands.)

Fig.2 Immunofluorescence staining of nestin in mouse neuroepithelial cells by using anti-nestin
antibody.
Cells in culture were fixed with paraformaldehyde and reacted with anti-nestin antibody at 1/300
dilution and then with rhodamaine-conjugated donky anti-rabbit antibody as a second antibody.
2 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

References: This antibody was produced and used in ref.1 and used 1~4.
1. Sato Y et al (1998) Requirement for early-generated neurons recognized by monoclonal antibody
Lot1 in the formation of lateral olfactory tract. J Neurosci 18:7800-7810 PMID: 9742149 IHC
2. Nakashima K et al (2001) BMP2-mediated alteration in the developmental pathway of fetal mousebrain cells from neurogenesis to astrocytogenesis.” Proc Natl Acad Sci USA 98: 5868-5873 PMID:11331769 IF
3. Shimozaki K. et al (2003) Involvement of Oct3/4 in the enhancement of neuronal differentiationof ES cells in neurogenesis-inducing cultures. Development 130, 2505-2512. PMID: 12702663 IF
4. Aizawa T. et al. (2011). Neural stem cell-like gene expression in a mouse ependymoma cell linetransformed by human BK polyomavirus. Cancer Sci. 102:122-9. PMID: 21073635 IF, IHC

3 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/