Run ﬁnished
Export PDF Report.fast5 ﬁle writing errors
encountered. MinKNOW cannot
start an experiment from thispoint. Please restart thecomputer, and start the runagain.

There was an error writing a.fast5 ﬁle. MinKNOW will try toﬁnish this experiment, although
the output might be corrupt.
Please stop your experiment,restart your computer, and start
the run again.

Disk too low to continue.
Stopping data acquisition.
Please clear some space andre-start the experiment

Disk usage alert – you only
have 1360 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.

Disk usage alert – you only
have 1394 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.



Disk usage alert – you only
have 1577 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.



Disk usage alert – you only
have 1511 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.

Disk usage alert – you only
have 1556 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.

Duty Time

MN23152

Summary of channel states over time
Bucket size (minutes)

10080
State Time Equivalent (%)

Apply

509070

Auto scale bucket size

Display channels proportionately

MN2315260
5040

MN23152

302010ZymoBIOMICS® HMW DNA Standard
050m

5h9h 10m

13h 20m



Sequencing

17h 30m
21h 40m
Time



Pore

1d 1h 50m

RecoveringMore

1d 6h

1d 10h 10m

Inactive

MN23152

Unclassiﬁed

Mux Scan Results•

MN23152

Channel counts per category after each mux scanHigh Molecular
Weight: DNA standard is > 50 kb in size.
1400
1600

MN23152

Channel count

1200
Accurate Composition:
Composition is cross-validated with multiple types of measurements.
1000
800

MN23152

600
400
200
00m

4h 30m9h

13h 30m18hTime Active

22h 30m

1d 3h

1d 7h 30m

Species


48500
15000

 InactiveMore

7000
4000

ThroughputBenchmark Long-ReadCumulative
Metagenomics
Throughput of run over time

3000
2500
2000

1.2 M
1M

Long-Read Metagenomics Standard. The ZymoBIOMICS®
HMW DNA
800 K Standard is composed of genomic DNA from sevenbacteria and one yeast and is >50 kb in size, as confirmed byK®
Agilent600Tapestation
.

1500
1200

2 days ago

2 days ago

2 days ago

Total Reads

Reads

10h

13h 20m 16h 40m

Called Skipped Reads

Enterococcus faecalis
600

20h

23h 20m

1d 2h

1d 6h

1d 9h

7.8

14

10.9

14

11.2

14

18.8

14

19.6

14

17.8

14

13.2

20.63

2 days ago

Disk usage alert – you only
have 1759 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.

Listeria monocytogenes
MN23152

Reads

2 days ago

Bacillus subtilis Disk usage alert – you onlyBases

1d 12hhave 1649 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.

Saccharomyces cerevisiae

Read Length Histogram

MN23152

Summary read length distribution

650 MB600 MB

2 days ago

Disk usage alert – you only
have 1715 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.

High Quality Long-Read Data
MN23152

550 MB500 MB

450 MB
400 MB
350 MB
300 MB250 MB
200 MB
150 MB
100 MB
50 MB
0B
0B

16.2 KB

32.4 KB

48.6 KB

64.8 KB
81 KB
Estimated Read Length in Bases

97.2 KB

113.4 KB

129.6 KB

otherwise your run will stop
prematurely.
MN23152

Basecall
Heatmap of read length vs basecall quality score
40,00030,00020,00068101214161820Cat. No.



5,000

10,000

ZymoBIOMICS® HMW DNA Standard

D6322

Size5000 ng

20,00025,00030,00035,00040,00045,000info@zymoresearch.com

www.zymoresearch.com

2 days ago

Disk usage alert – you only
have 1915 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.
MN23152

15,000

2 days ago

Disk usage alert – you only
have 1866 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.
MN23152

Quality Score [4,20]

50,000100,000

Product

2 days ago

Disk usage alert – you only
have 1760 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.

High Quality Long-Read Sequencing. The ZymoBIOMICS®
2 days ago
HMW DNA Standard was MN23152
sequenced
using the Oxford
Nanopore MinION™ andthe Disk
Ligation
Sequencing kit (LSK109)
usage alert – you only
have 1953
MB of space
free,
for library preparation. Readlength
histogramshows anwhich is insuﬃcient for the run.
approximate average of 24 kb
withkb recorded.
Pleasefree up>125
some space,

Bucket Width (B) 1620(B)Base Read (bases) [0,70000]

14

Staphylococcus aureus

100

Time

GenomeCopy

Salmonella enterica

Called Failed Reads

TheoreticalComposition (%)

GenomicDNA

Disk usage alert – you only
have 1635 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.

MN23152250
6h 40m2 days ago

have 1742 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.

Called Passed Reads

200 K

3h 20m2 days ago

Disk usage alert – you only
Escherichia coli

400

0MN23152

2 days ago

Pseudomonas aeruginosa
MN23152 2 days ago

900

400 K

Total Estimated Bases•

2 days ago

Long-Read Sequencing
Ready: Ideal to benchmark 3rd generation sequencing and metagenomic measurements
2048
1800
(e.g. Oxford Nanopore& PacBio).

•

2 days ago

2 days ago

Disk usage alert – you only
have 1996 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.

Toll Free: (888) 882-9682
MN23152

2 days ago

Disk usage alert – you only
have 2164 MB of space free,which is insuﬃcient for the run.
Please free up some space,otherwise your run will stop
prematurely.

Cat. No. DS1709