PROTOCOL

Accell siRNA

Accell siRNAsiRNA
Accell siRNA delivery media

Accell siRNA

Accell siRNA
Accell siRNA deliverymedia

Accell siRNA
96

1.
3

1.

Accell siRNA delivery media

2.

Accell positive control siRNAsiRNA3.

Accell Non-targeting siRNA control

4.

2.

15-75

3.

96

4.

37℃

5

5.

5X siRNA buffer

1

6.

1X siRNA buffer
RNase
100 μM siRNAsiRNAhttps://horizondiscovery.com/
a. siRNAsiRNA1X siRNA Buffer
freesiRNAb.
3 5n=3

Accell siRNAAccell positive control siRNAcontrol2Acell Non-targeting siRNAAccell siRNA

96

2313100 μL

RNase
1X siRNA buffer

4RNase-c.

1.25

d.

37℃siRNA7090

7.
7.5 μL 100 μM siRNA
750 μL Accell siRNAdelivery mediadelivery mix
delivery mix
Accell siRNA
96
1 μM
18.
siRNAAccell siRNA Delivery Media

100 mL
500 mL

B-005000-100
B-005000-500

5X siRNA Buffer

100 mL

B-002000-UB-100

Molecular Grade RNase-freewater

100 mL

B-003000-WB-100

horizondiscovery.com

9.
10.

37℃

100 μL delivery mix (AccellAccell siRNA delivery media)
572mRNA

962

7.

Accell siRNA delivery media

750 μLsiRNA

Accell siRNA
1 μM

96
Accell siRNA8.

Accell siRNAdelivery media
1.
2.

5X siRNA buffer

9.
RNase
1X siRNA buffer

1X siRNA buffer
100 μM siRNAmRNA
96

37℃

100 μM siRNA

72

RNase

70μL

52c.
siRNA

37℃

10.

4siRNA
https://horizondiscovery.com/
a. siRNAsiRNA1X siRNA Buffer
RNase-freesiRNAb.
3 5d.

Accell siRNA delivery media
100 μL 96

Accell siRNAAccell siRNA

196

10-2.5
Accell delivery mix48

Accell siRNA

90

3.

2

7.5

4.

siRNA

72

37℃

5.
1x PBS
delivery media

Accell siRNA

Accell siRNA

2-3

96

Ficoll6.

Accell
Accell siRNA5
24

Accell siRNA delivery media
96

1

Accell siRNA
Accelldelivery mix
Accelldelivery mix

24

1

2

horizondiscovery.com

024

48

Accell delivery mix

Accelldelivery mix

72

96

FAQ
Accell siRNA
Accell siRNA delivery media

Accell siRNA delivery media

Accell siRNA
0-2.5

Accell siRNA
Accell siRNAdelivery media
Gibco B-27 serum-free neuronal supplementsiRNAAccell siRNA delivery media

Accell siRNA delivery media

0-2.5%

AccellAccell siRNA delivery media

Accell siRNA
Delivery mix Accell siRNAAccell siRNA delivery mediadelivery mix

48
72

Accell siRNA

1 μM

1 μM

500 nM

200 nM

1 μM

Accell delivery mix Accell siRNAAccell siRNA delivery media

4℃

90

Accell siRNAGelatin

Mouse ESD3 (embryonic stemcells)

Poly-L-lysine

HeLa, SH-SY5Y, MCF-70.001% FibronectinHeLa

0.001% Fibronectin

HUASMC

0.001% Fibronectin

HUVEC

HycloneTM Cell Boost 1HeLa, SH-SY5Y

HycloneTM Cell Boost 2HeLa, SH-SY5Y

Hyclone

TMCell Boost 3

HeLa, SH-SY5Y

HycloneTM Cell Boost 4

HeLa, SH-SY5Y

HUVEC complete media ( 2%)Astrocyte basal media (ABM;HUVEC)NHA (normal human astrocytes)

2.5 %MCF-7, SH-SY5Y, NIH/3T3

NeurobasalTM media
B27, GibcoTM

GibcoTM B27 neuronal supplement
Accell siRNA

Accell siRNAS8252755

US8501706

1US8188060

US8415466

For more information

horizondiscovery.com/contact-us©2023 Horizon Discovery Group Ltd and its affiliated companies. All rights reserved. Revvity is a trademark of Revvity, Inc., registered in the UnitedStates and other countries. Horizon Discovery is a trademark of Horizon Discovery Ltd. Dharmacon is a trademark of Dharmacon, Inc. All Revvity,
Inc., Horizon Discovery Ltd and Dharmacon, Inc. trademarks are used with permission. Other product names and brand names may be trademarks
or registered trademarks of their respective owners.
B032-2006-v4