Anti-Pertussis Toxin antibody, rabbit serum
Product code64-030Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.09% sodium azide

PurityRabbit antiserum

Immunogen

Immunization was Initiated with toxoid and boosted with native toxin(BioAcademia 01-503)

IsotypeRabbit IgG

Reactivity

Bordetera pertussis strain TohamaSpecial notes

N/AApplication

1. Western blotting (1/2,000~1/10,000 dilution)
2. ELISA (1/10,000~1/20,000 dilution)
3. Dot blotting (1/2,000~1/10,000 dilution)
4. Immunoprecipitation (1/200~1/500 dilution)
5. Neutralising (Assay dependent)
Other applications have not been tested.

Background

Perrtussis toxin (PT) is a protein-based AB5-type exotoxin produced byBordeterra pertussis. PT catalyzes the ADP-ribosylation of the α subunits of
the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt andprevents intracellular signal transduction involving the G proteins.

PTconsists of one moplecule of each S1 (26 kDa), S2 (22 kDa), S3 (22 kDa), S5 (12kDa) and two molecule of S4 (12 kDa). This product was highly purified (>90%pure) from Bordetella pertussis strain Tohama by the method of Skelton &Wong1). Cytotoxicity of the PT was confirmed by morphological alteration ofCHO cells after treatment with 0.1 ng/ml of PT (see the Figure below).

Data Link

UniProtKB Pertussis toxin subunit1, subunit2, subunit3,subunit4, subunit5

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 64-030 Anti-Pertussis Toxin antibody, rabbit serum

Fig.1. Detection of perussis toxin in culture medium of Bordetera pertussis strain Tohama by Western
blotting using anti-perussis toxin antibody.
1.

Culture medium of Bordetera pertussis. SDS-PAGE, silver-stained

2.

Purified pertussis toxin (200 ng). SDS-PAGE, silver-stained3.

Western blot of culture medium of Bordetera pertussis as in 1.

4.

Western blot of purified pertussis toxin (10 ng)

The toxin consists of five subunits as indicated by S1 to S5.

Fig.2. Titration of antibody reactivity of anti-Pertussis antiserum by direct ELISA
Plate was coated with 100 μg of pertussis toxin per well and 100 μl of

the antiserum at theindicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG
conjugated with HRP was added as 2nd antibody. Color was developed with TMB as substrate.
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Fig.3. Titration of pertussis toxin by direct ELISA using anti-pertussis toxin antiserum
ELISA plate is coated with indicated amounts of pertussis toxin per well. Antiserum was used at
1/12,500 dilution. ELISA was performed as in Fig.2. Dynamic range was 100 pg to 10 ng under theseconditions.
References: Alouf JE & Popoff MR (Ed.) The comprehensive Sourcebook of Bacterial
Protein Toxins 3rd Ed. Academic Press (2006)
Related Products: #64-031 Anti-Pertussis Toxin antibody, rabbit polyclonal

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