Minimization of sample diffusion - Optimum
for semi-microcolumn analysis!

Optimized for Biological Samples
Sample Cooling Function
The autosampler features a sample coolingfunction, useful for analysis of biologicalsamples.
Metal-free Structure
The flow channels are made ofpolyetheretherketone (PEEK) resin, allowing
analysis of samples of strong adsorptive
nature, such as proteins or otherphysiologically active samples.
Pre-column Labeling Function

Pre-labeling function(OPA labeling of aminoacids)

Selection of Sample Vials
Vial Holder(1) 96-Well Microplate(2) Conventional Vial(3) Osaka Soda Vial (100 Samples)(4) Osaka Soda Vial (200 Samples)*Osaka Soda vials are available in 50 µL and 250 µL types.

Complete Rinsing System
A complete rinsing system is adopted for the sampling mechanism to eliminate contaminationand carryover.
Double injection rinsing function
The sample loop and tubing are rinsed.

Post-injection rinsing function
Rinsing liquid can be chosen from any positions.
Syringe, needle, injection port, and injection valve are rinsed.

System configuration with double-injection rinsing function

High-precision Analysis at High Sensitivity
Small Dead Volume Structure
The capacity of the injection valve has been reduced to 1.9 µL, virtually eliminating diffusion ofthe sample. Semi-microcolumns of 1.0-mm I.D. are also available.
Influence of dead volume on semi-microcolumn HPLC
Comparison of Peak Efficiency of Standard Compound (naphthalene) between Nanospace andConventional Instrument (Retention time: 12 min)High Precision and Endurance
High precision is maintained even at small injection volumes (RSD less than 1% at 1 µL
injection). A minute injection of 100 nL is also possible. The standard sampling volume ranges
from 0.1 to 80.0 µL.
*Up to 400 µL injection can be made by using multiple collection injection.

Efficient Sampling System
Polypropylene (PP) sample vials (50 µL & 250 µL) are suitable for biological samples. Valuable
samples will not be wasted.
50 µL vial: Minimum required sample volume = Injection volume + 2 µL
Easy Operation with Key Pad
Programming is set by using the numerical key pad.
Networked System Control
Remote operation from a WindowsTM PC is possible.
Specifications
Product No.

3023

Product NameAutosampler

SystemTotal injection system of variable sampling volumeSample Vial

250 µL PP
Optional: 2 mL glass, 96-well microplate, and 50 µL PP

SamplingMicrosyringe

Gas-tight Type 100 µL

SampleInjection
Volume100 µL syringe: 0.1 to 400 µL in 0.1 µL increments

Injection
Precision

RSD less than 1% at 1 µL injection

Repeated
Injection1 - 10 times for each vialMax. Sample
Number100 (100-sample vial holder with 250 or 50 µL PP vials),
200 (200-sample vial holder with 250 or 50 µL PP vials),
50 (50-sample vial holder with 2 mL glass vials), or96 (96-sample vial holder with 96-well microplate)

SampleCoolingElectronic refrigeration: 4 to 20°C (variable setting)OperationalPressure

35 MPa (max.)

Interruptible
Analysis

Included in Standard Model

Rinsing
Solution Port

Included in Standard Model

Error Selfdiagnosis

Included in Standard Model

Input/Output
Signals

Integrator start, auto-zero, start, end, stop, error signal, SYSCON

Power

AC 100 V ±10%, 50/60 Hz, 350 W

Dimensions

258(W) x 460(H) x 487(D) mm

WeightAbout 31 kg