Anti-t-Actin 2 /ACTLA antibody, rabbit polyclonal
Product code73-079Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum

ImmunogenSynthetic peptide DGPAKKASDQASMQT conjugated KLH

IsotypeRabbit IgG

Reactivity

Mouse

Special notes

N/AApplication

1. Western blotting (1-10µg/ml)

Background

Actins play essential roles in cellular morphogenesis. In mice, the T-actin1(testis specific action 1) and 2 (testis specific action 2) genes, which encode actinlike proteins, are specifically expressed in haploid germ cells. Both TACTIN1/ACTLB and T-ACTIN2/ACTL7A have also been cloned. The
orthologous genes in humans are present on chromosome 9q31.3 as intronlessgenes. Defects of germ cell-specific genes can introduce infertility without
somatic function impairment. T-ACTIN1 and 2 are specifically expressed in thetestis.
Molecular mass: Mouse 49.4 kDa

Data Link

UniPlotKB Q9QY84 (ACL7A_MOUSE)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 73-079 Anti-t-Actin 2 /ACTLA antibody, rabbit polyclonal

Fig.1 Western Blot of t-Actin 2 protein
Applied sample; 50µg of mouse testis whole lysate.
Primary antibody; 1µg/ml of anti-t-Actin 2 antibody
Secondary antibody; 1/10,000 dilution of goat anti-rabbit IgG antibody HRP-conjugated, ab97051)
Molecular mass of mouse t-Actin 1 ; 49.4 kDa

Fig.2 Immunohistochemical staining of t-Actin 2 protein in mouse testis section using anti-t-Actin2antibody.
mouse testis ( 4% PFA) HMPS-41-10; genostaff)
Anti-t-Actin2 antibody was used at 50µg/ml. The second antibody was used Alexa-Fluor 488 labeledanti-rabbit IgG (green). Nuclei was stained with DAPI (blue).

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Fig.3. Immunohistochemical staining of T-ACTIN 2 in mouse testes. (ref.2)
The testes were stained with anti-T-ACTIN 2 rabbit antiserum. Testicular cross sections wereexamined under a fluorescence microscope using a blue-filter for 4′,6′-diamidino-2-phenylindole(A, C, and E) or a green-filter for the FITC-labeled secondary antiserum (B, D, and F). Blots weremade using preimmune control serum (A and B and anti-T-ACTIN 1 antiserum (C–G). Bar = 100
μm for C. Higher magnification of the stage VII/VIII tubules show the amorphous positive stainingat the tubule centers (arrow, D). G represents a merged image of E and F. Bar = 10 μm
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Reference:
1. Tanaka H, et al. Genetic Polymorphisms within The Intronless ACTL7A and ACTL7B Genes
Encoding Spermatogenesis-Specific Actin-Like Proteins in Japanese Males. Int J Fertil Steril.(3)245-249 (2019) PMID: 31310081. WB
2. Tanaka H, et al. Novel actin-like proteins T-ACTIN 1 and T-ACTIN 2 are differentially expressedin the cytoplasm and nucleus of mouse haploid germ cells. Biol Reprod (2) 475-482 (2003) PMID:12672658.WB IF
Related product73-Anti-t-Actin 1 /ACTLB antibody, rabbit polyclonal

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