Anti-SHIPPO 1/Ciliary microtubule associated protein 1A antibody, rabbit polyclonal
Product code73-081Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum

ImmunogenPurified mouse SHIPPO1(1aa-202aa) with Hit tag expressed in E.coli.

IsotypeRabbit IgG

ReactivitymouseSpecial notes
Application

1. Western blotting (1-10µg/ml)
2. Immunofluorescence staining (1-20µg/ml)

Background

Shippo 1 cDNA was isolated in haploid germ cell-specific cDNA clone from asubtracted cDNA library of mouse testis. Shippo 1 cDNA encodes a highlyhydrophobic putative protein, SHIPPO 1, which exists in the cytoplasm ofspermatids and along the entire length of the sperm tail of mature spermatozoa.
Further localization of SHIPPO 1 to the ODF and FS was ascertained byfractionation of the sperm proteins. The homology between the mouse andhuman sequences suggests that SHIPPO 1 proteins have an importantfunctional role in the sperm tail. (Ref.1)
Molecular mass: 27.6 kDa. Detected 32 kDa in mouse testis by WB.(Ref.1)

Data Link

UniPlotKB Q920N1 (CMA1A_MOUSE)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 73-081 Anti-SHIPPO 1 (Ciliary microtubule associated protein 1A ) antibody, rabbit
polyclonal

Fig.1 Western Blot of SHIPPO 1 protein
Applied sample; 20µg of mouse testis whole lysate.
Primary antibody; 1µg/ml of anti-SHIPPO 1 antibody
Secondary antibody; 1/10,000 dilution of goat anti-rabbit IgG antibody HRP-conjugated, ab97051)
Molecular mass of SHIPPO 1; 32 kDa

Fig.2. Immunohistochemical staining of SHIPPO 1 in mouse testis with anti-SHIPPO 1 antibody.
Section of formalin-fixed and paraffin embedded mouse testis was reacted with anti-SHIPPO 1
antibody at 1/1000 dilution. Nuclear DNA was stained with DAPI (center) and merged image is shown.

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Fig.3 Immunohistochemical analysis of SHIPPO 1 protein (Ref.1)
Anti-SHIPPO 1 antibody recognized the tail of an elongating spermatid protruding toward the lumenof the seminiferous epithelium (A). Control using the preimmune serum shown in B. Signal was alsopresent in cytoplasmic droplets of sperm just before they left the seminiferous epithelium (C).
Epididymal sperm was positive at the tail and cytoplasmic droplets were discarded (D). No signal wasobserved with the preimmune serum (E). SHIPPO 1 signal was detected along the entire length of
the tail of mature sperm in the vas deferens. F) Phase-contrast and G) immunofluorescent microscopic
pictures. Arrows indicate cytoplasmic droplets. Bars = 10 μm
Reference: This product has been used in the following publication.
1. Carlos Egydio de Carvalho, Tanaka H, Nishimune Y, et al. Molecular cloning and characterizationof a complementary DNA encoding sperm tail protein SHIPPO 1. Biology of Reproduction; 66(3)785–795 (2002) PMID: 11870087. WB, IF

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