Storage recommendations for samples collected using OMNIgene®
products from DNA Genotek™
The preservation and storage of microbiome samples is an important consideration for any study design.
The table below summarizes recommendations for short-term handling and long-term storageof unprocessed samples collected in the various OMNIgene® products offered by DNA Genotek™.

OMNIgene•GUT(OM-200, OMR-200)

Room temperature storage(20°C to 26°C/68°F to 79°F)

Freezer storage(-20°C or -80°C/-4°F or -112°F)

Storage at room temperature:
Fecal samples collected in OMNIgene•GUT(OM-200, OMR-200) can be stored at room
temperature for up to 60 days.

Storage at -20°C or -80°C:
Fecal samples collected in OMNIgene•GUT(OM-200, OMR-200) can be stored in their
original tube at -20°C and -80°C within 60 daysof collection for up to 1 year. For longer storage,
OMNIgene•GUT samples should be vigorously
mixed, aliquoted into cryovials and stored at -80°C.

IMPORTANT: Storing at 4°C (39°F) is
NOT recommended for fecal samples
collected in OMNIgene•GUT.

Freeze-thaw cycles:
OMNIgene•GUT will preserve the microbialcommunity structure and maintain DNA
integrity for up to 6 freeze-thaw cycles.

OMNIgene•ORALfor DNA (OM-501)

Storage at room temperature:
Saliva samples collected in OMNIgene•ORAL(OM-501) can be stored at room temperaturefor up to 1 year.
IMPORTANT: Storing at 4°C (39°F) isNOT recommended for saliva samples
collected in OMNIgene•ORAL.

Storage at -20°C or -80°C:
Saliva samples collected in OMNIgene•ORAL(OM-501) should be incubated at 50°C (122°F)
for 1 hour in a water bath or 2 hours in a dry
incubator, mixed well by inversion, aliquoted
into cryovials and stored at -20°C or -80°C within
1 year of collection.

Freeze-thaw cycles:
OMNIgene•ORAL will maintain DNA
integrity for up to 3 freeze-thaw cycles.

OMNIgene•ORALfor DNA & RNA(OM-505, OME-505)

Storage at room temperature:
Saliva samples collected in OMNIgene•ORAL(OM-505, OME-505) can be stored at room
temperature for up to 21 days.
IMPORTANT: Storing at 4°C (39°F) is
NOT recommended for saliva samples
collected in OMNIgene•ORAL.

Storage at -20°C or -80°C:
Saliva samples collected in OMNIgene•ORAL(OM-505, OME-505) should be incubated at 50°C
(122°F) for 1 hour in a water bath or 2 hours in a
dry incubator, mixed well by inversion, aliquoted
into cryovials and stored at -20°C or -80°C
within 21 days of collection.

Freeze-thaw cycles:
OMNIgene•ORAL will maintain DNA and RNA
integrity for up to 3 freeze-thaw cycles.
Continued on next page

OMNIgene•ORAL(OMR-110, OMR-120)

OMNIgene•VAGINAL(OMR-130)

Room temperature storage(20°C to 26°C/68°F to 79°F)

Freezer storage(-20°C or -80°C/-4°F or -112°F)

Storage at room temperature:Samples collected in OMNIgene•ORAL(OMR-110, OMR-120) and OMNIgene•VAGINAL(OMR-130) can be stored at roomtemperature for up to 30 days.

Storage at -20°C or -80°C:
Samples collected in OMNIgene•ORAL(OMR-110, OMR-120) and OMNIgene•VAGINAL(OMR-130) should be treated with Proteinase K
and incubated,1 aliquoted into cryovials and storedat -20°C or -80°C within 30 days of collection.

IMPORTANT: Storing at 4°C (39°F) is NOT
recommended for samples collected inOMNIgene•ORAL or OMNIgene•VAGINAL.

IMPORTANT: Ensure samples are vortexed andthe swab has been pressed against the collection
tube’s inner wall to remove excess liquid prior toaliquoting or if transferring into cryovials.
Freeze-thaw cycles:
OMNIgene•ORAL and OMNIgene•VAGINAL
will preserve the microbial community
structure and maintain DNA and RNA
integrity for up to 3 freeze-thaw cycles.

OMNIgene•SKIN (OMR-140)

Storage at room temperature:Samples collected in OMNIgene•SKIN(OMR-140) can be stored at room
temperature for up to 30 days.
IMPORTANT: Storing at 4°C (39°F) isNOT recommended for samples collectedin OMNIgene•SKIN.

Storage at -20°C or -80°C:
Samples collected in OMNIgene•SKIN(OMR-140) should be treated with Proteinase Kand incubated,1 aliquoted into cryovials and storedat -20°C or -80°C within 30 days of collection.
IMPORTANT: Ensure samples are vortexed andthe swab has been pressed against the collection
tube’s inner wall to remove excess liquid prior toaliquoting or if transferring into cryovials.

Freeze-thaw cycles:OMNIgene•SKIN kits will preserve themicrobial community structure and maintain
DNA integrity for up to 3 freeze-thaw cycles.

1Add 5 µL of Proteinase K (80 mg/mL) to the 1 mL sample collection tube and vortex. Incubate for 1 hour at 50°C in a water bathor 2 hours in a dry incubator.
Recommended Proteinase K: Epicentre PK (Cat. no. MPRK092) or QIAGEN® Protease (Cat. no. 19155)

Technical support is available Monday to Friday (9h00 to 17h00 ET):
Toll-free (North America): 1.866.813.6354, option 6
All other countries: +1.613.723.5757, option 6
Email: support@dnagenotek.com

Some DNA Genotek products may not be available in all geographic regions.
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All DNA Genotek protocols, white papers and application notes are available in the support section of our website at www.dnagenotek.com.
PD-PR-01036_Issue 2/2021-01© 2021 DNA Genotek Inc., a subsidiary of OraSure Technologies, Inc., all rights reserved.

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