Progress made simple!

USER’S GUIDEAILABLE IN
AV

SINGLE &MULTICHANNEL

Contents

Page

1-

INTRODUCTION

32-PARTS CHECK LIST AND ACCESSORIES

4

3-

GETTING STARTED

44-DESCRIPTION

55-PIPETTING JUST LIKE WITH PIPETMAN

6

6-PIPETTING MODES

7

7-

PIPETTING SPEED CONTROL

11

8-

PIPETMAN TIPS

11

9-

GUIDELINES FOR GOOD PIPETTING

12

10 - PERSONALIZE YOUR PIPETMAN M

14

11 - GOOD LABORATORY PRACTICE FEATURES

15

12 - PIPETMAN M POWER MANAGEMENT

15

13 - CONFIGURATION

17

14 - MAINTENANCE OF YOUR PIPETMAN M

18

15 - CLEANING AND DECONTAMINATION

22

16 - LEAK TEST

25

17 - TROUBLESHOOTING

25

18 - SPECIFICATIONS

26

19 - SAFETY PRECAUTIONS AND LIMITATIONS OF USE

27

20 - REPLACEMENT PARTS

28

APPENDICES29

EC DECLARATION OF CONFORMITY

33

WARRANTY

35-2-

ENGLISH

1 INTRODUCTION
Congratulations on the purchase of PIPETMAN® M the natural evolution of PIPETMAN, the world standard for pipetting.

Mode-button

PIPETMAN M is a new motorized member of the PIPETMAN® family
from Gilson. It is a fusion of PIPETMAN’s renowned accuracy, precisionand robustness as well as user-friendly functions to simplify yourprofessional life.
PIPETMAN M is so easy to use that you will have the feeling that you
are using a PIPETMAN Classic:• Push-button: set the volume by turning the button left or right andpipette with a simple push.
• Mode-button: to access additional features like repetitive mode, mix
function or easy readjustment.

Push-button

That’s all you need to perform your pipetting tasks easily!
PIPETMAN M is nothing less than a PIPETMAN and much more:• Easy to use: only one push-button for all pipetting functions.
• More pipette techniques for a large number of applications:standard pipetting, repetition, mixing and reverse.
• Maximal comfort: motorized aspiration and dispensing, easy ejectionof tips to avoid repetitive strain injuries (RSI).
• Minimal effort: PIPETMAN M is much lighter than other motorized
pipettes (RSI).
• “No user” effect: high reproducibility and accuracy due to motorized
pipetting.
• More fun for better performance.
PIPETMAN M is available in 10 models covering a range
from 0.5 µL to 1000 µL:

-3-

Model
Reference
P10M
F81001
P20M
F81002
P200M
F81003
P1000M
F81004P8x20M
F81005P12x20M
F81006
P8x200M
F81007P12x200M
F81008P8x300M
F81011
P12x300M
F81012Range
0.5 µL to 10 µL
2 µL to 20 µL
20 µL to 200 µL
100 µL to 1000 µL
1 µL to 20 µL
1 µL to 20 µL
20 µL to 200 µL
20 µL to 200 µL
10 µL to 300 µL
10 µL to 300 µL

Color code
Red
Pale yellow
Yellow
Blue
Pale yellow
Pale yellow
Yellow
Yellow
GreenGreen2 PARTS CHECK LIST AND ACCESSORIES
Contents:

Accessories:

Just take a moment to verify that the following
items are present:

The following items are available separately:• POWER CARROUSEL 5-position charging carrousel for single and multichannel models
FB1001• PIPETMAN M pipette,• Power Supply 5V with AC adapter and cable,• 4 Battery Tags,• 4 Ejector Tags,• Tip ejector extension (P10M model only),• User’s Guide,• Quick Guide,• Safety bag,
• Certificate of conformity (including bar-code
sticker),• Lubricant (except for P10M, P8x20M, P12x20M,
P8x200M, P12x200M, P8x300M, P12x300M)).

• Battery Tags (set of 4)
F807013• Ejector Tags (set of 4)
F807014
Please contact your Gilson Distributor forthese accessories.

3 GETTING STARTEDPIPETMAN M is provided with a minimal battery charge. Before using your new PIPETMAN M, we strongly
recommend you to fully charge the battery. PIPETMAN M charges 80 % of its full battery capacity inless than an hour, it takes three hours to fully charge the battery. Please refer to PIPETMAN M PowerManagement (Chapter 12) for charging procedure.
Volume range

Switch on your PIPETMAN M:
Press the push-button to activate the pipette. The start-up screen appears:
The pipette will do a self calibration test, the firmware resets the piston.
During initialization, displayed on the screen, you will see the volume
range, version of firmware and the number of readjustments.
Your PIPETMAN M is preset on PIPET mode and is now ready to pipette(see Chapter 5).
PIPETMAN M goes into sleep mode after 3 minutes of inactivity. The
display will shut off. Just press the push-button to reactivate the pipette,your last setting and pipetting mode will appear on the screen.

-4-

Version of
firmwareNumber of readjustments

ENGLISH

4 DESCRIPTIONPIPETMAN M Single and Multichannel
modelsUpper part
 Push-button and volume adjustment knob
 Mode-button: direct access to all menufunctions Tip ejector button
 Display Elastomer plug to cover the battery
charging port
 Identity-tag clip
 Identity-tag window Connecting nut – attaches handle tolower part
 Tip ejector: removable to access tip-holder
 Optimized tip-holder to reduce tip fitting
and ejection forces, removable for cleaning and servicing
 Ejector clip Ejector support
 Cover Ejector spacer
Reset:To reinitialize your pipette, simultaneously
press on the push-  and mode-  button
for at least 5 seconds







 Pipetting Mode Indicator for aspirationand dispense Battery status
 Purge Volume

Switch Off:

To switch off your PIPETMAN M, press thepush-button  for at least 5 sec.
Display
The PIPETMAN M display is an OLED screen.
Usually, it shows the current mode and operation step, pipetting volume, battery indicatorand piston status (aspirated and dispensed
volume) in real time.

Warning messages appear in theplace of volume.
-5-5 PIPETTING JUST LIKE WITH PIPETMAN
Using PIPETMAN M is as easy as using a mechanical PIPETMAN. Your PIPETMAN
M is set by default on “PIPET Mode”, that means forward pipetting. You canselect a volume and start to pipette.
Switch on
Press push-button.
Adjust the volume setting• Hold your PIPETMAN M in pipette position.
• Turn the push-button half way, the screen will display: “click to change vol.”• Now press the push-button; the volume on the display will start blinking,you can now adjust the volume:• Turn the push-button either clockwise to decrease volume or counterclockwise to increase volume, like indicated on the button.
• Press the push-button one time when finished. The volume is locked.
You are ready to pipette!
The tip can be emptied at any time during a pipetting cycle. To doso, turn the push button quickly: the message “click to abort” willappear on the screen. Click on the push button to validate: the liquid
will be dispensed and an automatic purge followed by piston reset
to zero will occur.
PIPET Mode
This is the classic pipette mode (forward pipetting), your pipette is simply
aspirating and dispensing.
Application note: You can use the PIPET Mode for all standard applications like DNA extraction, Plasmid Isolation,
Cloning, Dilution, PCR, qPCR and many others. You can easily pipette aqueous liquids like buffer, chemical solutions(MgCl2, KCl …) and biological samples like blood, DNA and RNA.

• Fit a tip suitable for the model of
PIPETMAN M that you are using(preferably use PIPETMAN TIPS for
assurance of accurate and precise
results; refer to Chapter 8).

You must always fit a tip
before using any pipette!

• Press the push-button to aspirate the selected volume.
• To dispense: Click (press and release) the push-button. Three things happen: sample
dispense, automatic purge and piston reset to zero.
		or Keep your thumb pressing the push-button until the end of the dispense
cycle: The piston reset takes place after releasing the push-button again.
-6-

V

0
Purge
START button
Optional STOPENGLISH

This feature enables you to remove the tip from the liquid, without aspirating anything.
• Eject tip by pressing the ejection button.
Your PIPETMAN M is ready again for next pipetting cycle while maintaining the volume and the lastsettings!
You have been pipetting like with your mechanical PIPETMAN and only needed 2 pushes on the
push-button!

6 PIPETTING MODES

Mode-button

PIPETMAN M offers more pipette techniques for a large number ofapplications: You will find all pipetting modes in the menu (for systemsettings refer to the Chapters 7 and 13). Access the menu by pushing theMode-button. To choose one of the pipetting modes, turn the pushbutton and confirm your selection by a simple click!
REPETITIVE Mode
The REPETITIVE Mode allows you to distribute asame volume repeatedly in predefined number(N) of equal aliquots. You supply the aliquot
volume (AV) and the pipette automatically calculates the number of aliquots possible from thenominal volume (NV) of  the pipette as follows:

Push-button

N = NV/AV (e.g. 100 μL x 10 for P1000M).
You also can decrease the number of repetitions (e.g. 100 μL x 10-(n ≥1) for P1000M).
Application note: The REPETITIVE Mode is ideal for dispensing aliquots, e.g.; dispensing a PCR master mix into PCR
tubes or 96 plates, pipetting elution buffer for DNA extraction, preparing a target for spectrometric analyses, distributing
loading buffer into samples, etc.

• Press Mode-button to access system menu, where you can choose between different pipette modes.
Select REPETITIVE Mode by turning the push-button. To confirm your selection click on the push-button.
• Turn the push-button half way, the screen will display: “click to changevol.”• Now press the push-button; the volume on the display will start to blink,
you can now adjust the volume:• Set the aliquot volume: click the push-button, set the volume by turning the push-button and click
again to confirm your settings.
• Set the aliquot number: The maximal number of aliquots has been calculated by your PIPETMAN
M. The number of aliquots flashes and you can decrease this number (N – (n ≥1)) by turning thepush-button. Click again to confirm your settings.
You are ready to pipette!
-7-1. Fit a tip suitable for the model of PIPETMAN M that you
are using (preferably use PIPETMAN TIPS for assurance of
accurate and precise results; refer to chapter 8).
2. Click push-button to aspirate total
volume. The volume aspirated will bea little bit more than required (extra
volume). The extra volume is necessary
to ensure equal operating conditions
for each dispensed aliquot.
3. To dispense: click the push-button.
DISCARD appears on the screen. Discard the extra volume and continue
dispensing the aliquots. PIPETMAN
M distributes the aliquot volume eachtime you click the push-button. The
number of aliquots left to dispense isdisplayed on the screen.

 A warning beep indicates the last aliquot!

You must always fit a tip
before using any pipette!

DISCARD

ALIQUOTESPURGEV (+ extra vol)*V (vol)0
Purge
START button
Click to Purge

Optional STOP
Start Purge

Discard

4. To purge: Press the push button, PURGE appears on the screen, click again to purge and reset thepiston. Keep your thumb pressing the push-button until the end of the purge: the piston resettakes place after you release the push-button again. This feature enables you to remove the tipfrom the liquid, without aspirating anything.
5. Eject tip by pressing the ejection button.
MIX ModeThis is the classic pipette mode (forward pipetting) followed by a mixing
phase, composed of repeatedly aspirating and dispensing, as well as an
optional forward pipetting step.
Application note: The Mode MIX can be used to prepare a PCR master mix, enzyme restriction mix, protein solution, oligonucleotide dilution and to mix samples with gel loading buffer and so on…you can mix two different solutions or homogenize
one solution. If you work with higher viscosity than water (e.g. restriction enzyme) you may change the aspiration speed(refer to chapter 7). If you work with genomic DNA you should always pipette very carefully to avoid shearing and nicking.

•Press Mode-button to access menu, where you can choose between different pipette modes. SelectMIX Mode by turning the push-button, to confirm your selection click on the push-button.

•

Set the first volume, named VOLUME 1: click the push-button, set the volume by turning the pushbutton and click to confirm your selection. This is the first volume you will aspirate and dispense.
Set the second volume, named VOLUME 2: click the push-button, you can now set the second
volume by turning the push-button. Click again to confirm your settings.

 You can set the VOLUME 1 or VOLUME 2= 0 µL the step will not appear. If you set the VOLUME 1 and 2 = 0 µL thecycle will start directly with the MIX step.

•

Set the MIX volume: click the push-button, set the volume by turning the push-button. Confirm
your settings by a simple click. It is the volume which will be aspirated and dispensed repeatedly.
“MIX” as long as you press the push-button.
-8-

ENGLISH•

Set the additional pipetting volume, named VOLUME 3: You can choose a volume to be aspirated
and dispensed after the mixing step, so you can continue pipetting! Click the push-button, set thevolume by turning the push-button. Confirm your settings by a simple click.

 If you set the VOLUME 3 = 0 µL the cycle will stop after the MIX step.

You are ready to mix!
1. Fit a tip suitable for the model of PIPETMAN M that you
are using (preferably use PIPETMAN Tips for assurance of
accurate and precise results; refer to chapter 8).

You must always fit a tip
before using any pipette!

2. To aspirate VOLUME 1 press the push-button
3. To dispense: Click (press and release) the push-button: Three things happen: sample dispense, automatic
purge and piston reset to zeroor
 Keep your thumb pressing the push-button until the end of the dispense cycle: The piston resettakes place after you release the push-button again. This feature enables you to remove the tipfrom the liquid, without aspirating anything.
4. To aspirate and dispense the second volume “VOLUME 2”, repeat steps 2 and 3.
5. To mix: Press the push-button; as long as you keep your thumb pressing the button, the pipettecontinues mixing. Release the push-button to complete the current mixing cycle.
6. To purge: press push-button, PURGE appears on the screen, click again to purge and reset thepiston.
7. To pipette: If you have chosen a VOLUME 3 > 0 you can now aspirate and dispense this volume byrepeating steps 2 and 3.

 If you choose VOLUME1 = 0, step 2 won’t occur, if you choose VOLUME 2 = 0 µL, step 4 won’t occur, as well if you set
VOLUME 1 and 2 = 0 µL, step 1-4 will disappear. If you set VOLUME 3 = 0 µL step 7 will not occur.
Push-button pressed

V2

V1

V

0
Purge
START button

Optional STOP

Start Purge-9-

V3

REVERSE Mode
The REVERSE Mode is like reverse mode pipetting with a manual pipette.
During aspiration an amount of liquid is added. After delivery the excess
volume remains in the tip and is discarded.
Application note: The REVERSE Mode is ideal for pipetting viscous liquids; liquids with high vapour pressure or thosewhich tend to foam. Your PIPETMAN M aspirates a selected volume and an excess. This excess compensates for the liquidthat remains as a film inside the tip during dispensing. For example: protein extraction, cell lysis, plasmid isolation, cell
culture, buffer preparation and many others…
Electrophoresis gels loading protocol: First change the pipetting speed of your pipette (see chapter 7). Aspiration canbe done by using the standard speed (by default speed 6). Dispensing should be done very slowly and carefully, to prevent
swirling and spilling of the samples, so select the lowest speed 1. Your pipette is now ready to load your gel! Please beaware that if you purge without pulling out the tip air bubbles could get into your gel!

• Press Mode-button to access system menu, where you can choose between different pipette modes.
Select REVERSE Mode by turning the push-button, to confirm your selection click the push-button.
• Set the volume: click the push-button, set the volume by turning the push-button and click again
to confirm your settings.  
You are ready to do reverse pipetting!
1. Fit a tip suitable for the model of PIPETMAN M that you
are using (preferably use PIPETMAN TIPS for assuranceof accurate and precise results; refer to Chapter 8).
2. Click push-button to aspirate the selected volume. An
amount of liquid equal to the amount of purged air isadded, the volume aspirated will be a little bit morethan the set volume.

You must always fit a tip before
using any pipette!

V (+ extra vol)*3. Click push-button to dispense the volume, the additionally amount of liquid remains in the tip.
4. To purge: press push-button, PURGE appears on thescreen, click again to purge and reset the piston. Keepyour thumb pressing the push-button until the endof the purge: the piston reset takes place after you
release the push-button again. This feature enables you
to remove the tip from the liquid, without aspirating
anything.
5. Eject tip by pressing the ejection button.

- 10 -

0
Purge
START button
Optional STOP

Click to Purge
Start Purge

ENGLISH

7 PIPETTING SPEED CONTROLPIPETMAN M is set by default on speed 6. You may need to change the speed of aspiration or dispensing
depending on your application. You can change aspiration speed and dispensing speed independentlyof one other: from very slow to very fast (speed 1 – speed 6). Your speed selection is memorized foreach pipetting mode until you change it again.
• Press Mode-button to access system menu, where you can find the different pipette modes, thespeed menu and configuration menu.
• Select SPEED by turning the push-button, to confirm your selection click on the push-button.
• Set ASPIRATION SPEED: aspiration speed is blinking, set the speed by turning the push-button andclick to confirm your selection.
• Set DISPENSING SPEED: dispensing speed is blinking, set the speed by turning the push-buttonand click to confirm your selection.
• After selecting the speed you go automatically back to pipetting mode.

8 PIPETMAN TIPSPIPETMAN TIPS are made to the highest specifications; strict quality control is maintained throughout
the manufacturing process. These tips are used to calibrate PIPETMAN M, therefore for optimum performance we recommend using PIPETMAN TIPS with your PIPETMAN M.
However, PIPETMAN M also offers you a high compatibility with a large
number of other tips. For more information please feel free to contact yourGilson Distributor.

Gilson logo

ID Number

PIPETMAN TIPS have the Gilson logo engraved on their collar, ensuring thatyou have a genuine Gilson product. To ensure accuracy and precision, Gilson’s Quality Assurance System focuses on the following critical parameters.
• PIPETMAN TIPS are made from pure polypropylene (virgin, metal-free,
to avoid the possibility of contamination). They are available sterilized and with filters.
• Sterilized PIPETMAN TIPS are certified free of detectable RNases, DNases, DNA, RNA, and proteases.
• Optimized shape (revised collar for optimum sealing, thin walls, and fine point), making them easier
to mount, more flexible, with no vortexing, and improved precision.
• PIPETMAN TIPS are free from even microscopic defects, especially at the orifice. All surfaces aresmooth and hydrophobic, thereby avoiding the excessive retention of liquids that causes poor accuracy and a lack of precision.
• Mold and cavity references are marked on the collar, ensuring the traceability. For quality assurance
purposes batch numbers appear on all packages (bags and boxes).

- 11 -

• They form an air-tight seal with the tip-holder,preventing the leaks that cause poor accuracy anda lack of precision.
• PIPETMAN TIPS (except filter tips) may be autoclaved at 121°C for 20 minutes at 0.1 MPa.
Do not autoclave PIPETMAN Filter Tips, they
have been sterilized!
To ensure the best performance fromyour Gilson pipette, you shoud ALWAYS
use PIPETMAN Tips (in accordance with
ISO8655) system, because PIPETMAN Tips
were used to establish the specifications.
* A plastic adapter is required to eject D10 and DF10
tips (short tips). The adapter is supplied with P10M.
No adapter is required when using DL10 and DFL10
tips (long tips).

9 GUIDELINES FOR GOOD PIPETTINGPIPETMAN Tips to use for best results
Pipette model

Tips model

Range

Color code

P10M

D10*
DL10
DF10
DFL10

0.1 – 10 μL
0.1 – 20 μL
0.1 – 10 μL
0.1 – 10 μL

Red

P20M

D200
DF30

2 – 200 μL Pale yellow
2 – 30 μL

P200M

D200
DF200

2 – 200 μL
2 – 200 μL

Yellow

P1000M

D1000
D1200
DF1000DF1200100 – 1000 μL
100 – 1200 μL
100 – 1000 μL
100 – 1200 μL

Blue

P8x20M, P12x20M

DL10
DFL10
DF30
D200

0.1 – 20 μL Pale yellow
0.1 – 10 μL
2 – 30 μL
2 – 200 μL

P8x200M, P12x200M

D200
D300
DF200
DF300

2 – 200 μL
20 – 300 μL
20 – 200 μL
20 – 300 μL

Yellow

P8x300M, P12x300M

D300
DF300

20 – 300 μL
20 – 300 μL

GreenAspirate and dispense
1) Fit new PIPETMAN Tip for the best results (see Chapter 18).
For single channel models, push the tip-holder into thetip using a slight twisting motion to ensure a firm andairtight seal.
For multichannel models,PIPETMAN Tips are best fitted
from the patented ROCKY RACK available only in our
TIPACKs and TOWERPACKs. ROCKY RACK is the domeshaped part of the pack that contains the tips. ROCKYRACK makes it easy to securely fit the tips to a multichannel
pipette, ensuring an airtight seal on all channels without
the need to use undue pressure or to touch the tips.
2) Pre-rinse the tip. Pre-rinsing consists of aspirating the first
volume of liquid and then dispensing it back into the same
vessel (or to waste). Subsequent volumes that you pipettewill have levels of accuracy and precision within specifications. Some liquids (e.g. protein-containing solutions andorganic solvents) can leave a film of liquid on the inside the- 12 -

ENGLISH

wall of the tip; pre-rinsing the tip minimizes any errors that may be related to this phenomenon.
3) Hold the pipette vertically and immerse the tip in the liquid (see page 13). Press the push-button
to aspirate the set volume of liquid. Wait a few seconds (time depends on model, see page 13);then withdraw the pipette-tip from the liquid. You may wipe any droplets away from the outsideof the tip using a medical wipe, however if you do so take care to avoid touching the tip’s orifice.
4) Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°).
Press the push-button. Wait for at least a few seconds before releasing the push-button to expel
any residual liquid from the tip. While removing the pipette draw the tip along the inside surfaceof the vessel.
5) Eject the tip by pressing firmly on the tip ejector button.
General Guidelines for Good Pipetting
a. Make sure that you fit new tips.
b. Each new tip should be pre-rinsed with the liquid to be pipetted.
c. When aspirating, keep the tip at a constant depth below thesurface of the liquid (refer to Table).
d. Change the tip before aspirating a different liquid, sample, orreagent.
e. Change the tip if a droplet remains at the end of the tip fromthe previous pipetting operation.
f. Liquid should never enter the tip holder. To prevent this:• Never turn the pipette upside down,

Immersion Depth and Wait Time		
Model
Immersion Depth Wait Time(mm)(seconds)
P10M
P20M
P200M
P1000M
P8x20M, P12x20M
P8x200M, P12x200M
P8x300M, P12x300M

1
2-3
2-4
2-4
2-3
2-3
2-3

1
1
1
2-3
1
1
1

• Never lay the pipette on its side when there is liquid in the tip(s),g. The Gilson Stand Adapter (see Chapter 2) is recommended for use with the CARROUSEL and SINGLE
Pipette Holder to store your PIPETMAN M pipette in the vertical position. Alternatively the POWERCARROUSEL can be used to store and charge up to 5 PIPETMAN M pipettes.
h. When pipetting liquids with temperatures different to the ambient temperature, pre-rinse the tip
several times before use in order to reach equilibrium between the temperatures of the liquid andthe pipette’s dead-volume.
i. For volatile liquids you should saturate the dead-volume by aspirating and dispensing the liquid
repeatedly before aspirating the sample.
j. After pipetting acids or other corrosive liquids that emit vapors, clean the pipette, as described in
Chapter 15.
k. The pipette can be used between + 4 °C and + 40 °C, but the
specifications may vary (see Chapter 18).
l. Do not pipette liquids having temperatures above 50 °C or
below 4 °C.

- 13 -

Extreme temperatures
can affect accuracy andprecision!

10 PERSONALIZE YOUR PIPETMAN M
Tip-ejector Extension for use with P10M

Slot

In order to eject D10 tips, tip ejector extensions are supplied with P10M
pipettes.
The tip-ejector extension which is made of PVDF (polyvinylidene Fluoride), is autoclavable.
To ﬁt a tip-ejector extension:• Hold the pipette with the push-button upright.
• Hold the extension with the slot upright.
• Slide the extension over the tip-holder.
• Push the extension firmly onto the end of the tip-ejector until it clicks
into place (see opposite).

To fitTo remove

To remove a tip-ejector extension (see opposite):• Hold the pipette in one hand and grip the extension with the other.
• Gently twist the extension (either direction) and pull it away from thepipette.

Short tips

Long tips

Personal Label – Name and/or Application Tag:Your PIPETMAN M offers you two options to mark your pipette. You canpersonalize your pipette with a name tag and/or an application tag:
Window-Tag:• Remove the window by inserting a small screwdriver in the access slot.
• Position the name tag on the pipette• Clip the window back in place.
Window-tag

Clip-Tag:

Clip-tag• Pull off the clip.
• Position the name tag into the clip
• Clamp the clip back in place.

- 14 -

ENGLISH

11 GOOD LABORATORY PRACTICE FEATURES
Your PIPETMAN M is fully compliant to ISO8655 standard and is CE marked (for IVD and EMC directives).
PIPETMAN M includes the following GLP features.
General• Lockable volume.
• Useful volume-range is printed on the push-button.
• Volume range is displayed on the screen.
• Serial number is engraved on the body (handle) of the pipette and encoded in the firmware.
• Bar code: on the box and with the certificate (can be transferred).
• Personalization by nametags (for marking application and/or user name), see Chapter 10.
Cycle counters• From last volume setting (to count the number of cycles in the current “run”).
• From manufacture and since the last service (see Chapter 14).
• By indicators (R1, R2, R3 … Rx) each time pipette is readjusted.
• Maintenance intervals can be specified by weeks or number of cycles elapsed.
Alarms• Low battery warning.
• Service is overdue warning (see Chapter 14).
• Service Alert can be set “On” or “Off”.

12 PIPETMAN M POWER MANAGMENT
The PIPETMAN M battery has been designed to ensure 900 pipetting cycles (750 for PIPETMAN M Multi
200 µL and 300 µL) at maximum speed (up to nine 96 well plates) between two charge cycles. For service continuity when needing more capacity, your PIPETMAN M can also be used while charging withthe same performance as a fully charged pipette!
USB port

Battery Charging
PIPETMAN M charges 80 % of its full battery capacity in less than an hour, it takes three hours to fully
charge the battery.

Plug Charge your pipette by using the power supply:- Connect the AC adapter to a suitable AC
power supply- Plug the adapter into the USB port of the   
pipette.
- The pipette starts charging.
- 15 -

Stand adapter

 Charge your pipette by using stand adapter(see Chapter 2):- First place the stand adapter on your Gilson
SINGLE pipette holder or CARROUSEL.
- Remove the plug. Now place your pipetteon the stand adapter and plug the powertransformer into the stand adapter.
- The pipette starts charging.

 Battery charging of PIPETMAN M using the

POWER CARROUSEL:- First install the POWER CARROUSEL as
described above.
- Remove the plug. Now place your pipetteon the charging position, please ensure thatthe pipette is properly fitted to the contacts
ont the top of the carrousel (a proper fitting
will trigger a beep sound).
- The pipette starts charging.

Working with charger connected to the pipette

U s e o n l y t h e o r i g i n a l p owe rtransformer supplied by Gilson. Useof an incompatible power supply candamage your PIPETMAN M!

PIPETMAN M can also be used for pipetting whilecharging.
- Plug the power supply into your pipette.
- Press the push-button to start your pipette.
- PIPETMAN M is ready to pipette.

 You can also recharge PIPETMAN M by USB with your com-

Never work with PIPETMAN M whilecharging by computer!

puter; however it will take much longer than by power supply.

Low Battery Warning:
PIPETMAN M has a low battery alert. As the battery runs down, the LOW
BATTERY message appears.The warning is replaced by a blinking symbolof an empty battery. If it is not recharged, the pipette will switch off after awhile. Settings will not be lost.
If the battery is completely discharged (= black screen, no operation) please connect the pipette to
the charger for at least five minutes, then a “batt. too low” message will appear. The pipette will display
the last settings used a few minutes later and will continue charging.
Changing the battery

PIPETMAN M uses Lithium-Ion battery.
Operation on the battery is done atuser’s risk only. Opening the pipette
voids the warranty!

If the following symptoms occur, please contact
your Gilson Service Center for battery change:- 16 -

ENGLISH- PIPETMAN M battery won’t charge or will not operate even if connected to the charger at least for
10 minutes.
- PIPETMAN M battery provides you very short cycle time or permanently needs to be used in connected mode.

13 CONFIGURATIONEnter the menu using the Mode-button. You will find not only the pipetting programs (PIPET, REPETITIVE, MIX and REVERSE) and the speed
control but also the menu “CONFIGURATION”, including “SERVICE” and“ADJUSTMENT” (refer to Chapter 14).

Mode-button

The navigation of the menus is always done in the same way  Make
your selection by rotating the push-button, enter and confirm witha simple click!
The configuration menu allows you to set up the following items:
1. Volume limit: Here you can fix the maximal volume of the pipette(by default = nominal volume). For example you
have a P200M pipette, but you would like to use it withyour PIPETMAN DIAMOND Filter tips DF100, you can
set the maximal volume at 100 µL and you won’t risk
contamination of your pipette.

Push-button

2. Beeper:

You can switch the tone of your pipette on or off.

3. Contrast:

You can choose between values of 1 to 5 to set up the contrast of the screen.

4. Service

see Chapter 14.

5. Adjustment

see Chapter 14.

14 MAINTENANCE OF YOUR PIPETMAN M
PIPETMAN M requires very little maintenance. However, to ensure pipette accuracy, precision androbustness for timeless use please proceed periodically with a Two-minute Inspection as recommended by Gilson.
Your PIPETMAN M allows you to:• Get service information.
• Readjust the pipette to user settings.
• Return to factory settings.
• Replace spare parts (for detailed information see Chapters 14 and 19).
• Prepare the pipette for cleaning or autoclaving by“disassembly”of the parts specified (see Chapter 15).
- 17 -

Service Information
PIPETMAN M provides you with all required service information. It will help you establish an easy
diagnosis and plan any service operation with your accredited Gilson Service Center.
You will find in the “service” menu following sub menus:
a. Disassemblyb. Service Info
c. Service Settings
d. Pipette Info
The navigation of the menus is always done in the same way  Make your selection by rotating
the push-button, enter and confirm with a simple click!
a. Disassembly:
The Multichannel models should not be disassembled: only the push-button and the tip-ejector canbe replaced.
Only the lower part of the Single models can be disassembled, the push-button, the connecting nut
and the tip-ejector can be replaced.
This option allows you to disassemble the lower part safely (protection of the piston and the actuator).
If you select DISASSEMBLY, the following screen appears:
Now, you can remove the lower part of your pipette with complete security.
b. Service Info provides you with maintenance information; you cannot modify any of the items:• Number of pipetting cycles since last maintenance.
• Number of weeks since last maintenance.
c. Service Settings gives you the option to organize your maintenance, you can set following service
points:• Number of cycles until next maintenance.
• Number of weeks until next service.
• Service date.
• Activate or deactivate the Service alert.

 Changes of service settings will reset all the service info to zero.
d. Pipette Info provides information about your pipette, you cannot modify any of the items:• Version of firmware.
• Serial number.
• Total number of cycles (one cycle: up and down of the piston).
• Number of readjustments carried out by this pipette.

- 18 -

ENGLISH

Adjustment
Press the mode-button to enter the menu. Select “configuration” by rotating the push-button and click
to confirm your selection. Enter the sub-menu in the same way and select the “adjustment” menu.
The adjustment menu allows you to access the following items:• Standard Readjustment• Reset Settings
Standard Readjustment
This menu allows you to adjust the pipette using 3 calibration points: 10 %, 50 % and 100 % of nominal
volume (in according with ISO8655 recommendations).
You may want to calibrate your pipette for solutions with a density, viscosity, surface tension or vapour
pressure that are different to that of water. To return to the factory settings, choose “Reset Settings”,
your PIPETMAN M will be reset to the three factory calibration values which are permanently stored
in the pipette’s firmware.
In accordance with ISO 8655 Gilson recommends a gravimetric procedure for pipette calibration. Thisgravimetric method is used to establish the mean mass of a given volume of water (taking into accountevaporation losses, where necessary). After converting the mean mass to a volume (using the Z factor,
see appendix II), enter the measured volumes(s) into the pipette’s memory and the software readjusts
the pipette accordingly. This method requires the strict monitoring of environmental conditions andthe use of routinely controlled equipment that is adapted to the volume being measured.
Conversion to volume must take into account the density of the liquid as well as evaporation during
the cycle time. For each measurement, the corresponding volume (Vi) can be calculated as follows:
Vi = (Wi + e) Z

Wi is the weight as read on the balancee is the mean evaporation loss during the cycle time
Z expressed in L/mg, is a conversion factor incorporating density of water buoyed in air,at test temperature and barometric pressure (see Appendix II)

For volumes greater than 20 μL, the evaporation factor can be disregarded.
For more information please refer to “Verification Procedure for Accuracy and Precision” which you candownload from the Gilson website (www.gilson.com).
Maintenance operations(disassembly of your PIPETMAN M to change and clean parts)
It is best to inspect your pipette regularly and to routinely
clean and change parts as required. To help you to keep
up a regular schedule and in the interests of GLP, you canconfigure your pipette to display an alarm before servicing
is due (refer to Service Settings, page 18).

- 19 -

The following maintenance
operation should only be done
when the pipette is in Mode
DISASSEMBLY (see page 18)!

Maintenance Warning
PIPETMAN M notifies you, when maintenance isdue (service alert is set by default to “ON”). The
following message will appear:
after validation by a click, the following message
appears:
If you click to confirm within 10 sec. the pipette willautomatically switch to “disassembly” mode so you
can carry out your maintenance safely.
Wait longer than 10 seconds and the option to havea reminder in one week will appear:
Click to confirm. Your pipette will be operational again.  
Maintenance operation for Single channel models
 Tip-Holder and Tip-ejector
These parts must be changed if they are damaged. You may also
remove these parts for cleaning or decontamination purposes.
 Changing the Tip-ejector
1) Keep the tip-ejector button depressed and grip the top of
the tip-ejector with the other hand.
2) Gently rotate the tip-ejector counter clockwise and separate
its connector from the operating rod.
3) Pull the tip-ejector away from the body of the pipette.
4) Clean or autoclave the tip-ejector and refit or replace it byreversing the procedure.
 Changing the Tip-holder (lower part)
After removing the tip-ejector, you may remove the lower part of the tip-holder, which is more likely
to become contaminated or damaged than the upper part. Removal of the lower part is shown below;
for the upper part see “Removing the Piston” (special precautions are necessary).
1) Gently rotate the lower part of the tip holder counterclockwise to unscrew it from the upper part.
2) Separate the parts and remove the O-ring (see “Changing
Tip-holder
O-ring”)(upper)3) Clean and if required autoclave the lower part of the tipTip-holder
Pistonholder (autoclaving: 20 min. at 121°C and 0.1MPa)(lower)4) If required, lubricate the piston (see “How to Lubricate the
Piston”) and fit a new O-ring.
5) Screw the two parts together, making sure that the twoparts are fully tightened, by hand.
6) Refit the tip-ejector.

 After autoclaving the tip-holder and tip-ejector may change color, however this has no impact on the performance.
- 20 -

ENGLISH Changing the seal and/or O-ring (see Chapter 20)
The O-ring is positioned on the piston; it should not be autoclaved, if
worn or damaged in any way, it must be replaced.

O-ringTo access the O-ring, remove the tip-ejector and unscrew the lower partof the tip-holder. You should now be able to remove the O-ring from thepiston. Sometimes, the O-ring may be found in the recess at the topend of the lower part of the tip-holder. If required, lubricate the piston(see “How to Lubricate the Piston”) then fit a new O-ring by sliding it onto the piston. Reassemble thepipette. Depending on the pipette model the dimensions of the O-ring will vary (refer to Chapter 20).
 Servicing the Piston
You may remove the piston-assembly to clean, lubricate, or change the piston.
 Removing the Piston
1) Remove the tip-ejector and (optionally) the lower
part of the tip-holder. If you remove the lower
part, take care to remove the O-ring as describedin “Changing the O-ring”.

The specifications of the pipettemust be checked after changing
any part!

2) Unscrew the connecting nut (turn by hand, counterclockwise).
3) Gently remove the connecting nut and upper tip
holder.
4) Pull off the piston assembly from the body of thepipette – separate the parts (see below).
5) Clean and autoclave (if required) the piston andholder, together with any other parts that may need
to be treated in the same way (see Chapter 15).

Don’t pull on the piston to remove
the assembly!

 In the case of P1000M, the piston holder, piston andguide are a unit. Don’t try to separate them!

6) Lubricate the piston, see below.
7) Reassemble the piston and guide; then carefully insert the assembly into the body of the pipette.
The guide should hold the piston assembly inside the body of the pipette. You can hear a “click”
when the piston is back in place.

 Take care not to touch the piston, and that it is dust-free. If needed, clean the assembly with a dust-free cloth.

Tip-holder (lower)

Tip-holder (upper)

Piston- 21 -

Piston holder

Guide

Connecting nut8) Reassemble the upper part of the tip-holder and theconnecting nut, and then refit to the body of thepipette by rotating the connecting nut clockwise
until it is finger tight. Fit the O-ring (for P10M, theseal) and reassemble the lower part of the tip-holder.
Refit the tip ejector.

Guide

Connecting nut

The seals for P10M are fragile and can be used only once. So, after unscrewing the lower part of the
tip-holder you must fit a new seal.
 How to Lubricate the Piston (except P10M)
Use only Gilson lubricant (ref: F2070902, as supplied).
Squeeze a small quantity from the tube onto a clean, lintless cloth. Use the cloth to transfer the lubricant to the piston. Ensure that the piston is evenly lubricated and that you wipe away any excess– remember only a fine film of lubricant is required(over the entire piston).
Maintenance operation for Multichannel models
For multichannel models, the lower part should not bedisassembled: only the push-button, the connecting
nut and the tip-ejector can be replaced.
 Changing the Tip-ejector
To remove the tip-ejector, keep both ejector locks
depressed. Pull the tip-ejector down.
To refit the tip-ejector, gently re-insert the tip-ejector
vertically into the rails of the ejector support. Pull lightly
on the tip-ejector to check the position.
 Replace the ejector spacer
1) Gently press the tabs from the ejector spacer andremove it from the tip-ejector.
2) Insert the ejector spacer and click it to the tipejector.

15 CLEANING AND DECONTAMINATION

Use alcohol (ethanol or isopropanol)
to clean up the dirt and dust on the
tip-holder!

PIPETMAN M is designed so that the parts normally incontact with contaminants can easily be cleaned anddecontaminated.

Liquid must not enter the handleof the pipette!

As part of your quality system, you may have procedures for pipette decontamination. We recommend that you refer to “Decontamination Procedures for Gilson Pipettes”, which you can downloadfrom Gilson’s website.
- 22 -

ENGLISH

If you use chemical solutions for decontamination or detergents for cleaning, other than those specified below, you should check with your supplier that the solution or detergent is safe for use with oneof the following materials: PP (Polypropylene), PBT (Polybutylene Terephthalate), PC (Polycarbonate),
POM (Polyoxymethylene), PVDF (Polyvinylidene Fluoride), PEI (Polyetherimide) and stainless steel.
Cleaning
The pipette must be cleaned, as described below, before it is decontaminated. For cleaning your
PIPETMAN M you may use a simple soap solution or any of the solutions mentioned in“Decontamination
Procedures for Gilson Pipettes”.
Single channel models
 External
1) Remove the tip-ejector for cleaning (see Chapter 14).
2) Replace the elastomer plug to protect battery charging port.
3) Wipe the entire pipette with a soft-cloth or lint-free tissue impregnated with soap solution, toremove all dirty marks. If the pipette is very dirty, a brush with soft plastic bristles may be used.
4) To rinse, wipe the entire pipette with a soft-cloth or lint-free tissue impregnated with distilled water.
5) Leave to air dry.
 Internal
The following components only can be immersed in a cleaning solution: tip-ejector, tip-holder,connecting nut and piston.
1) Disassemble the pipette as described in Chapter 14.
2) Set aside the handle in a dry and secure location.
3) Clean the individual components using an ultrasonic bath (20 minutes at 50 °C) or with a soft-clothand brushes.
4) Rinse the individual components with distilled water.
5) Leave the parts to dry by evaporation or wipe them with a clean soft-cloth or lint-free tissue.
6) Lubricate the piston and reassemble the pipette according to the instructions given in Chapter 14.
Multichannel models
The following components only can be immersed in a cleaning solution: tip-ejector, ejector locks
and ejector spacer.
1) Remove the tip-ejector and the ejector spacer (see Chapter 14).
2) Immerse the tip-ejector, ejector locks and ejector spacer in the cleaning solution or wipe themwith a soft cloth or lint-free tissue impregnated with the cleaning solution.
3) Rinse the components with distilled water.
4) Leave the parts to dry by evaporation or wipe them with a clean soft-cloth or lint-free tissue.
5) Refit the tip-ejector.
- 23 -

Please note that although the lower part of PIPETMAN M Multichannel can withstand a few numberof autoclaving cycles, we do not recommend autoclaving it.
Decontamination
Autoclaving - Single channel models
However, after separation from the body, any of thefollowing components of the volumetric module may
be autoclaved individually: tip-ejector, connecting nut,
tip-holders, pistons and seals (except O-rings).

The body (handle) of the pipette is
not autoclavable!

1) Clean the parts to be autoclaved, especially the tip-holders.
2) Put the parts in an autoclaving bag.
3) Autoclave for 20 minutes at 121°C and 0.1 MPa.
4) Check that the parts are dry before reassembling
the pipette.
5) Set the pipette aside to stabilize at room temperature for at least 6 hours.

The specifications of the pipettemust be checked after autoclaving/disassembling!

Chemical Decontamination - Single and Multichannel models
You may choose to decontaminate your pipette chemically, in accordance with your own procedures.
Whatever decontaminant you use, check that it is compatible with the plastics used in the construction of the pipette (see page 22).
 Parts that may not be immersed
1) Wipe the handle of the pipette with a soft-cloth or lint-free tissue impregnated with the chosen
decontaminant.
2) Wipe the handle of the pipette with a soft-cloth or lint-free tissue impregnated with distilled water.
 Parts that may be immersed
The following components only can be immersed in a decontaminant solution:• Single channel models ->tip-ejector, tip holder (both parts), connecting nut, piston (including
holder) and guide.
• Multichannel models -> tip-ejector, ejector locks and ejector spacer.
Please note that although the lower part of PIPETMAN M multichannel can withstand a few numberof autoclaving cycles, we do not recommend autoclaving it.
1) Disassemble the pipette as described in Chapter 14.
2) Immerse the components in the decontaminant solution or wipe them according the instructions
given by the manufacturer or supplier of the decontaminant.
3) Rinse the individual components with distilled or sterile water.
4) Leave the parts to dry by evaporation or wipe them with a clean, lint-free tissue or soft-cloth.
5) Lubricate the piston and reassemble the pipette according to the instructions given in this chapter.
- 24 -

ENGLISH

16 LEAK TESTThis test may be performed at any time to check that the pipette does not leak, especially after
performing a maintenance or decontamination procedure. If a pipette fails this test, you should
replace the faulty part (e.g. O-ring, tip-holder...) and repeat this test, after making sure that the pipetteis correctly reassembled.
• Fit a PIPETMAN DIAMOND Tip,
• Set the pipette to the nominal volume,• Aspirate the nominal volume from a beaker of distilled water.
• Hold the pipette in the vertical position and wait for 20 seconds,• If a water droplet appears at the end of the tip there is a leak (see Chapter 17).
• If you see no droplet, re-immerse the tip below the surface of water,• The water level inside the tip should remain constant; if the level goes down there is a leak (seeChapter 17).
• For Multichannel models, check if the water level between each tip is the same.

17 TROUBLESHOOTING

Before returning any pipette to yourlocal Gilson Service Center, ensure that it
is completely free of chemical, biological,
or radioactive contamination. Please
use the included safety bag.

In case of malfunction, first reset the pipette bypressing on the push-button and the Mode-button
simultaneously at least for 8 seconds.
If the problem persists, you may consult the following table which identifies potential problems
and their solutions.
ProblemPossible Cause

ProblemPossible Cause

Pipette is not precise.

Unscrewed lower part of tip-holder.
Incorrect operator technique.
Worn O-ring.
Connecting nut is loose.
Damaged or corroded piston.
Damaged tip-holder.

20
13
21
21
21
20

Tips fall or don’t fit.

Low quality tips.
Damaged tip-holder.
Damaged tip-ejector.
Ejector spacer is damaged.
Tip-ejector is loose.
Dirty tip-holder.

11-12
19
19
22
20-22
22

No OLED display.

Pipette is in sleep mode.
Battery needs recharging.
Software needs to be reset.

4
15-16
5

No operation possible.

Battery needs recharging.
Software needs to be reset.

15-16
5

Calibration impossible.

Software needs to be reset.

5

Refer to page

Pipette is leaking sample. Worn O-ring.

21

Pipette won’t aspirate.

Worn O-ring.
Unscrewed lower part of tip-holder.
Damaged or corroded piston.
Damaged tip-holder.
Improper repair or assembly.
Connecting nut is loose.
Software needs to be reset.

21
20
21
20
20-22
21
5

Noisy operation.

Piston needs lubricating.

22

Pipette is inaccurate.

Improper repair or assembly.
Unscrewed lower part of tip-holder.
Pipette is out of adjustment.
Connecting nut is loose.

20-22
20
19
21
- 25 -

Refer to page

18 SPECIFICATIONSPIPETMAN M is a high quality pipette that offers excellent accuracy and precision; it is fullycompliant with ISO 8655 and is CE marked (forIVD and EMC directives).
The figures given in the “Gilson Maximum
Permissible Errors” table were obtainedusing “PIPETMAN TIPS”. These figures are only
guaranteed when using Genuine PIPETMAN
TIPS.
Each pipette is inspected and validatedby qualified technicians according to theGilson Quality System. Gilson declares thatits manufactured pipettes comply with therequirements of the ISO 8655 Standard, by
type testing. The adjustment is carried out
under strictly defined and monitored conditions (ISO 8655-6):• Basis of adjustment, Ex.
• Reference temperature, 20 °C• Relative humidity, 50 %• Barometric pressure, 101 kPa• Use of distilled water grade 3 (ISO 3696)• Ten measurements for each test volume,
which are Nominal Volume, 50 % of Nominal
Volume and 10 % of Nominal Volume.
(tested mode: PIPET, speed 6 with PIPETMAN Tips)

Maximum Permissible Errors
Model PIPETMAN Volume
GilsonISO 8655(Reference) tips used			 Systematic Random Systematic Random

error (μL) error (μL) error (μL) error (μL)
				P10M		
Min. 0.5 ± 0.04 ≤ 0.013 ± 0.12
(F81001)
DL10		
1 ± 0.025 ≤ 0.012 ± 0.12
			
5 ± 0.060 ≤ 0.020 ± 0.12
		
Max. 10 ± 0.080 ≤ 0.025 ± 0.12

≤ 0.08≤ 0.08≤ 0.08≤ 0.08P20M		
Min. 2 ± 0.075 ≤ 0.025 ± 0.20 ≤ 0.10
(F81002)
D200		
10 ± 0.100 ≤ 0.035 ± 0.20 ≤ 0.10
		
Max. 20 ± 0.150 ≤ 0.05 ± 0.20 ≤ 0.10
P200M		Min. 20 ± 0.40 ≤ 0.15 ± 1.60 ≤ 0.60
(F81003)
D300		
100 ± 0.80 ≤ 0.22 ± 1.60 ≤ 0.60
		Max. 200 ± 1.00 ≤ 0.26 ± 1.60 ≤ 0.60
P1000M		
Min. 100 ± 2.50
(F81004) D1000 		
500 ± 3.50
		
Max. 1000 ± 5.00

≤ 0.4
≤ 0.7
≤ 1.1

±8
±8
±8

≤ 3.0
≤ 3.0
≤ 3.0

Maximum Permissible Errors
Model PIPETMAN Volume
GilsonISO 8655(Reference) tips used			 Systematic Random Systematic Random

error (μL) error (μL) error (μL) error (μL)
				

P8x20M		
Min. 1 ± 0.08 ≤ 0.05
(F81005)
DL10		
10 ± 0.15 ≤ 0.10
P12x20M
Max. 20 ± 0.25 ≤ 0.12
(F81006)		

± 0.4
± 0.4
± 0.4

≤ 0.2
≤ 0.2
≤ 0.2

P8x200M		
Min. 20 ± 0.50 ≤ 0.16
(F81007)		
D200
100 ± 1.00 ≤ 0.30
P12x200M
Max. 200 ± 2.00 ≤ 0.50
(F81008)		

± 3.2
± 3.2
± 3.2

≤ 1.2
≤ 1.2
≤ 1.2

P8x300M		
Min. 10(F81011)		
30
D300
P12x300M
		
150(F81012)		
Max. 300

± 8.0
± 8.0
± 8.0
± 8.0

≤ 3.0
≤ 3.0
≤ 3.0
≤ 3.0

- 26 -± 1.00 ≤ 0.18
± 1.00 ≤ 0.18
± 1.50 ≤ 0.375± 2.40 ≤ 0.45

ENGLISH

19 SAFETY PRECAUTIONS AND LIMITATIONS OF USE
For safety reasons, it is important to observe the following instructions:
1. Battery and electrical specifications:
We strongly recommend you fully charge the battery before using the pipette.
PIPETMAN M is supplied with an AC adaptor that is suitable for your country. You must only usean original Gilson AC adaptor specific to this product. Charge the battery in the pipette, using theAC-adapter or the stand adapter.
Use AC-adaptor and stand adapter indoors.
PIPETMAN M uses Lithium-Ion battery. Operation on the battery is done at user’s risk only. Opening
the pipette voids the warranty.
Dispose of used batteries in accordance with legal regulations. Batteries may not be disposed ofwith household waste and may explode if disposed in fire !
• Li-ion battery pack; 1 Ah / 3.6 V
Charging time: approx. 3 hours for a fully discharged battery (80% in one hour).
• AC adaptor
Input voltage, country-specific: 100-240 V, 50/60 Hz (0.5A max.)
Output voltage: +5 V DC, 3.5A (17.5W max.)• Class II this device is double insulated.
2. Do not use PIPETMAN M in a potentially explosive environment
or with potentially explosive chemicals.
3. When pipetting infectious, radioactive, toxic and other hazardous solutions,please observe all the safety precautions (e.g. wear protective clothing, goggles and gloves) andregulations appropriate for your country.
4. Do not allow the liquid to enter the body of the pipette.
5. Storage conditions
Temperature: -20°C to 50°C - Humidity max: 80%
6. Temperature of use
Between 4°C and 40°C (specifications may vary).
7. Use only genuine PIPETMAN Tips and original Gilson’s accessories and spare parts.
If the equipment is used in a manner not specified by Gilson in the user’s guide, the protection
provided in the equipment may be impaired.
8. Equipment disposal
This equipment must not be disposed of with unsorted municipal waste. Instead, it isyour responsibility to correctly dispose of your waste equipment by handing it over toan authorised facility for separate collection and recycling. It is also your responsibility
to decontaminate the equipment in case of biological, chemical, and/or radiologicalcontamination so as to protect from health hazards the persons involved in the disposal
and recycling of equipment. For more information about where you can drop off yourwaste equipment for recycling, please contact your local dealer from whom you originallypurchased the product or your local council. By doing so, you will help conserve natural
resources and you will ensure that your waste equipment is recycled in a manner that
protects human health and the environment. Thank you.
- 27 -

9. PIPETMAN M can be used indoor and  outdoor,
if precautions are respected as described above in this handbook and in the GLP.

20 REPLACEMENT PARTS
Single channel models

Multichannel models
Ejectorlock

Ejector spacer

Tip-ejector
assembly

Description
Battery tag, 1x4
Ejector tag, 1x4
Power supply USB
Power tub coverBattery window
Ejector window
Button assembly
Ejector spacer
Ejector lockTip-ejector assembly

Description
P10M
Connecting nut
F807012Battery tag, 1x4
F807013Ejector tag, 1x4
F807014
Power supply USB
F807015
Power tub coverF807022
Battery window
F807005
Ejector window
F807006
Lubricant tube 3.5g		
Seal, 1x5
F161902
Seal & O-ring, 5 setsO-ring,1x5
Tip-holder, upper part F2070117
Tip-holder, lower part F2070218
Button assembly
F807001
Tip-ejector assembly F807008
Tip-ejector extension F2070903

P20M
F807012
F807013
F807014
F807015
F807022
F807005
F807006
F3070902
F144863
F2070117
F2070318
F807002
F807009-

P200M
F807012
F807013
F807014
F807015
F807022
F807005
F807006
F3070902
F2070501
F2070517
F2070518
F807003
F807010-

P1000M
F807012
F807013
F807014
F807015
F807022
F807005
F807006
F3070902
F2070601
F2070617
F2070618
F807004
F807011-

Piston assembly

F807018

F807019

F807020

P8x20M
F807013
F807014
F807015
F807022
F807005
F807006
F807112
F507001
F507008
F507005- 28 -

F807017P12x20M P8x200M P12x200M P8x300M P12x300M
F807013 F807013 F807013
F807013 F807013
F807014 F807014 F807014
F807014 F807014
F807015 F807015 F807015
F807015 F807015
F807022 F807022 F807022
F807022 F807022
F807005 F807005 F807005
F807005 F807005
F807006 F807006 F807006
F807006 F807006
F807112 F807003 F807003
F807116 F807116
F507003 F507001 F507003
F507001 F507003
F507008 F507008 F507008
F507008 F507008
F507006 F507005 F507006
F507005 F507006

ENGLISH

APPENDIX 1 - EXAMPLE OF A PERFORMANCE CHECK
Below is an example of how to evaluate the performance of PIPETMAN P10M at 1 µL.
4. Calculate the mean volume

1. Determine the mean value e of the evaporation loss ei that occurs during your pipetting
cycles. Proceed as described in Appendix 3
to determine ei

V = ( W+e ) x Z_
V_ = (0.964+0.018) x 1.0032
V = 0.985 µL

For a temperature of 21.5 °C and an air pressureof 1013 hPa, the Z factor is equal to 1.0032 µL/
mg (see table in Appendix 2).
5. Evaluate accuracy

E = V - V0

Systematic
error (E): V0 true value set on the instrument
2. Change the pipette tip and performthe first weighing. Then, keep a regularcycle and perform the 10 following
measurements.

wr = 0.957 mg
w1 = 0.968 mg
w2 = 0.960 mg
w3 = 0.984 mg
w4 = 0.942 mg
w5 = 0.969 mg

Relative
error
(E%):

E = 0.985 - 1 = - 0.015 µL

E% = (V - V0) x 100 / V0
E% = (-0.015 x 100) / 1 = - 1.50 %6. Evaluate precision (repeatability)

w6 = 0.966 mg
w7 = 0.955 mg
w8 = 0.972 mg
w9 = 0.958 mg
w10 = 0.967 mg

Standard Deviation (SDW)(Wi - W) 2
n-1
i=1
n

SDw =

wr rinsing measurement which isdisregarded for the calculation

2

SDw =

3. Calculate the mean weight

1
n-1

n
2

( Wi - W )
i=1

_
1 n
Wi
W=
n i=1

SDw =

n number of weighings
Wi weighing results_
W = (0.968+0.960+0.984+0.942

Random error (SDv) :2

2

SDw = 0.011 mg+ 0.969+0.966+0.955+0.972+ 0.958+0.967) / 10

SDv = SDw x Z_
W = 0.964 mg

SDv = 0.011 x 1.0032 = 0.011 µL- 29 -

2

2(0.968-0.964) + (0.960-0.964) + (0.984-0.964) +1 (0.942-0.964)2+ (0.969-0.964)2+ (0.966-0.964)2+22
2
9 (0.955-0.964)2+ (0.972-0.964) + (0.958-0.964) +(0.967-0.964)

APPENDIX 2 - Z FACTOR
The reference calculation equation is :Z = [1/(PW-PA)] [1-(PA/PB)]

Where :

 A = density of air at t°C.
P
PW = density of the test liquid at t°C.
PB = density of the balance weights. Use 8 g/cc for PB

 Weights conforming to International Recommendation N°33 of
OIML have been adjusted to give results when weighing in air as if thedensity of the weights were 8.0 g/mL.

Values of the conversion factor
Z (µL/mg) as a function oftemperature and pressure for
distilled water.

Temperature
(°C)

800

853

Air pressure (hPa)
907
960

1013

1067

15
15.5
16
16.5
17
17.5
18
18.5
19
19.5
20
20.5
21
21.5
22
22.5
23
23.5
24
24.5
25
25.5
26
26.5
27
27.5
28
28.5
29
29.5
30

1.00181.00181.00191.00201.00211.0022
1.0022
1.00231.00241.00251.00261.00271.00281.00301.00311.0032
1.00331.00341.00351.00371.00381.00391.00401.0042
1.00431.00441.00461.00471.00491.00501.00521.00181.00191.00201.00201.00211.0022
1.00231.00241.00251.00261.00271.00281.00291.00301.00311.0032
1.00331.00351.00361.00371.00381.00401.00411.0042
1.00441.00451.00461.00481.00491.00511.00521.00191.00191.00201.00211.0022
1.00231.00241.00251.00251.00261.00271.00281.00301.00311.0032
1.00331.00341.00351.00361.00381.00391.00401.0042
1.00431.00441.00461.00471.00481.00501.00511.00531.00201.00201.00211.0022
1.00231.00241.00251.00261.00271.00281.00291.00301.00311.0032
1.00331.00341.00351.00361.00381.00391.00401.00411.00431.00441.00451.00471.00481.00501.00511.0052
1.00541.00201.00211.0022
1.00231.00231.00241.00251.00261.00271.00281.00291.00301.00311.0032
1.00331.00351.00361.00371.00381.00391.00411.0042
1.00431.00451.00461.00471.00491.00501.0052
1.00531.0055- 30 -

1.00191.00201.00211.0022
1.0022
1.00231.00241.00251.00261.00271.00281.00291.00301.00311.0032
1.00331.00351.00361.00371.00381.00391.00411.0042
1.00431.00451.00461.00481.00491.00501.0052
1.0053

ENGLISH

APPENDIX 3 - EVAPORATION LOSS
Procedure for the determination of evaporation loss
Use the same distilled water, weighing vessel and balance as you will be using for the gravimetriccheck.
1. Half fill the weighing vessel with distilled water.
2. Cover the weighing vessel with its lid and place it on the balance using a pair of tweezers.
3. Aspirate a sample.
4. Tare the balance and take the weighing vessel out of the balance.
5. Take off the lid with tweezers.
6.	Dispense the sample into a dummy vessel.
7. Replace the lid on the weighing vessel and, using tweezers, replace the vessel on the balance.
8. Read the negative result e1 (record the absolute value).
9. Repeat steps 3 to 8, three times to obtain e2, e3, and e4.
10.	Calculate the evaporation loss e using the formula: In normal conditions, this value is usually between 0.01 mg
and 0.03 mg.

- 31 -

e = 1 (e1 + e2 + e3 + e4)
4

APPENDIX 4 - HOW TO CALCULATE VOLUMETRIC ACCURACY AND PRECISION“Accuracy” and “precision” are qualitative terms. The corresponding quantitative terms are“systematic error” and “random error”.
Evaluation of accuracy

Evaluation of precision

The specified accuracy is the limit to the systematic error, which is the difference between themean volume of actual measurements and thetrue value of the volume set on the instrument.

The specified precision is the limit to the random
error, which is the distribution of the measured
values around a mean value. For pipettes, precision refers to a within-series group of data, andtherefore to repeatability.

The systematic error (E) can be estimated asfollows :The random error is then quantified by the standard deviation of measurements performed at agiven volume setting under the same measuringconditions.

E = V - V0
Esystematic error

The standard deviation (SD or “s”) can be estimated as follows:

V0 nominal volume
V mean volume

1 n
Vi
V=
n i=1

(V - Vi) 2
n-1
i=1
n

SD =

Vi individually measured volume
n number of measurements

V mean volume

V=

The accuracy of a pipette can be expressed as apercentage of the nominal volume:

1 n
Vi
n i=1

Vi individually measured volume
n number of measurements

E% = V - V0 x 100
V0(minimum 10)

The precision of a pipette can also be expressed
as a percentage of the mean volume. This is
known as relative standard deviation (RSD) or
coefficient of variation (CV), and is estimatedas follows :The mean value and number of
replicates must be stated, and theexperimental procedure used must
be described in such a way that otherworkers can repeat it!

RSD =- 32 -SD
x 100
V

ENGLISHEC DECLARATION OF CONFORMITYGILSON S.A.S.
19, avenue des Entrepreneurs
BP 145 - 95400 VILLIERS LE BEL
FRANCE
Tel.: +33(0)1 34 29 50 00
Fax: +33(0)1 34 29 50 20

The company

Hereby certifies on its sole responsibility that the
products listed below:

PIPETMAN® M
P10M, P20M, P200M, P1000M
P8x20M, P12x20M
P8x200M, P12x200M, P8x300M, P12x300M

comply with the requirements of the following
European Directives:

98/79/EC*In Vitro Diagnostic Medical Devices
2004/108/EC
Electromagnetic Compatibility, EMC
2006/95/EC
Low Voltage Directive, LVD* Annex III, self declared

Villiers-le-Bel, April 26th, 2013

Atika El Sayed
General Manager

Hervé Le Dorze
Quality Manager- 33 -NOTES- 34 -

ENGLISH

WARRANTY
Gilson warrants this pipette against defects in material under normal use and service
for a period of 12 months from the date of purchase.
This warranty shall not apply to pipettes which are subject to abnormal use and/or
improper or inadequate maintenance (contrary to the recommendations given in the
User’s guide), including, but not limited to pipettes which have been subjected to physicaldamage, improper handling, or spillage or exposure to any corrosive environment. This
warranty shall also be void in the event pipettes are altered or modified by any party
other than Gilson or its designates. Gilson’s sole liability under this warranty shall belimited to, at Gilson’s sole option, repair or replacement of any defective componentsof pipettes or refund of the purchase price paid for such pipettes.
THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS ALL
OTHER WARRANTIES, WHETHER EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY AND ANY WARRANTIES OF FITNESS FOR A PARTICULARPURPOSE, UNDER NO CIRCUMSTANCES SHALL GILSON BE LIABLE FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF ANY
BREACH OF ANY EXPRESS OR IMPLIED WARRANTY.

- 35 -

LT801527/E- ©2013 Gilson SAS All rights reserved
April 2013
EnglishPrinted in France
Specifications subject to change without notifications - errors omitted.

www.gilson.com
sales@gilson.com
service@gilson.com
training@gilson.com
Gilson, Inc. World Headquarters
3000 Parmenter Street I P.O. Box 620027 I Middleton, WI 53562-0027, USA
Tel: (1) 800-445-7661 or (1) 608-836-1551 I Fax: (1) 608-831-4451
Gilson S.A.S.
19, avenue des Entrepreneurs I BP 145, F-95400 Villiers-le-Bel, France
Tel: +33 (0) 1 34 29 50 00 I Fax: +33 (0) 1 34 29 50 20