Anti-Leaf-FNR1 (Ferredoxin NADP Reductase, isoprotein 1) antibody, rabbit polyclonal
Product code

81-001

Size200 µg

Storage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum.

ImmunogenPurified recombinant maize leaf FNR1 protein.

IsotypeRabbit IgG

Reactivity

Plant leaf FNRs including FNR1 of Maize and Arabidopsis
Cross reacts with Maize L-FNR2 and L-FNR3, and Arabidopsis L-FNR2.
Reacts weakly with root FNR.

Special notes

Validation: Specificity has been validated by WB with recombinant full-size
maize FNR1 protein.

Application

1. Western blotting (1/500-1/2,000 dilution)
2. ELISA (assay dependent)

BackgroundFerredoxin-NADP reductase, leaf isozyme 1 (L-FNR1) plays a key role inregulating the relative amounts of cyclic and non-cyclic electron flow to meet thedemands of the plant for ATP and reducing power.
Subcellular location: Chloroplast

Data Link

UniProtKB Q9FKW6 (A. thaliana), Q9SLP6 (Z. mays)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 81-001 Anti-Leaf-FNR1 (Ferredoxin NADP Reductase, isoprotein 1) antibody, rabbit
polyclonal

Fig.1 Western Blot of L-FNR1 protein
Anti-L-FNR1 antibodywas used at 1/500 dilution. Second antibody (goat anti-rabbit IgG antibody
HRP-conjugated, ab97051) was used at 1/10,000 dilution.
1. Arabidopsis leaf extract, 10 μg2. Maize leaf extract, 10 μg
The molecular masses of mature forms of maize FNR1, FNR2 and FNR3 are 34.97, 35.57 and 34.7
kD, respectively (Ref 2)

Fig.2 Comparison of recombinant and native L-FNRs in maize extracts.
Western blotting was performed with anti-L-FRN1 antibody at 1/500 dilution
1. Recombinant maize FNR1 (34.97 kD)
2. Recombinant maize FNR2 (35.57 kD)
3. Recombinant maize FNR3 (34.7 kD)
4. Chloroplast fraction
5. Stroma fraction
6. Thylakoids fraction
This antibody has cross-reactivity with other L-FNRs.
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BioAcademia,Inc. Tel. 81-6-6877-2335

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References: This product has been used in the following publication.
1. Onda Y, Matsumura T, Kimata-Ariga Y, Sakakibara H, Sugiyama T, Hase T. “Differentialinteraction of maize root ferredoxin:NADP(+) oxidoreductase with photosynthetic and nonphotosynthetic ferredoxin isoproteins.” Plant Physiol. 2000, Jul;123(3):1037-45. PMID: 10889253
WB ; Maize
2. Okutani S, Hanke GT, Satomi Y, Takao T, Kurisu G, Suzuki A, Hase T. “Three maize leaf
ferredoxin:NADPH oxidoreductases vary in subchloroplast location, expression, and interaction
with ferredoxin.” Plant Physiol. 2005, Nov;139(3):1451-9. PMID: 16244136 WB ; Maize
3. Hanke GT et al. Multiple iso-proteins of FNR in Arabidopsis: evidence for different contributions
to chloroplast function and nitrogen assimilation. Plant, Cell & Environment. 2005, 28 (9): 11461157. Link file WB ; Arabidopsis
4. Hanke GT, Endo T, Satoh F, Hase T. “Altered photosynthetic electron channelling into cyclic
electron flow and nitrite assimilation in a mutant of ferredoxin:NADP(H) reductase.” Plant Cell
Environ. 2008, Jul;31(7):1017-28. PMID: 18410491 WB ; Maize
5. Twachtmann M, Altmann B, Muraki N, Voss I, Okutani S, Kurisu G, Hase T, Hanke GT. “Nterminal structure of maize ferredoxin:NADP+ reductase determines recruitment into different
thylakoid membrane complexes. Plant Cell. 2012, Jul;24(7):2979-91. PMID: 22805436 WB ; Maize,Arabidopsis

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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/