研究業績
樹木生物学・荒川圭太（平成２４年１月）
１）学術論文Wang D., Kasuga J., Kuwabara C., Fukushi Y., Fujikawa S., Arakawa K.: Presence ofsupercooling-facilitating (anti-ice nucleation) hydrolyzable tannins in deep supercooling xylem
parenchyma cells in Cercidiphyllum japonicum. Planta, DOI 10.1007/s00425-011-1536-3 (inPress)
Kuwabara C., Kasuga J., Wang D., Fukushi Y., Arakawa K., Koyama T., Inada T., Fujikawa S.: Change ofsupercooling capability in solutions containing different kinds of ice nucleators by flavonol
glycosides from deep supercooling xylem parenchyma cells in trees. Cryobiology, 63: 157-163(2011)
Wang D., Kasuga J., Kuwabara C., Fukushi Y., Fujikawa S., Arakawa K.: Characterization ofsupercooling-facilitating (anti-ice nucleation) hydrolyzable gallotannins in supercooling xylem
parenchyma cells in katsura tree (Cercidiphyllum japonicum).
Cryobiology andCryotechnology, 57: 147-151 (2011)
Ukaji, N., Kuwabara, C., Kanno, Y., Seo, M., Takezawa, D., Arakawa K., Fujikawa, S.: Endoplasmic
reticulum-localized small heat shock protein that accumulates in mulberry tree (Morusbombycis Koidz.) during seasonal cold acclimation is responsive to abscisic acid. Tree
Physiology, 30: 502-513 (2010)
Umebayashi, T., Utsumi, Y., Koga, S., Inoue, S., Matsumura, J., Oda, K., Fujikawa, S., Arakawa K.,Otsuki, K.: Xylem water-conducting patterns of 34 broadleaved evergreen trees in southern
Japan. Trees, 24: 571-583 (2010)
Kasuga, J., Fukushi, Y., Kuwabara, C., Wang, D.H., Nishioka, A., Fujikawa, E., Arakawa K., Fujikawa, S.:
Analysis of supercooling-facilitating (anti-ice nucleation) activity of flavonol glycosides.
Cryobiology, 60, 240-243 (2010)
Endoh, K., Kasuga, J., Arakawa K., Ito, T., Fujikawa, S.: Cryo-scanning electron microscopic study on
freezing behaviors of tissue cells in dormant buds of larch (Larix kaempferi). Cryobiology, 592:
214-222 (2009)
Fujikawa S., Kasuga J., Takata N., Arakawa K.: Factors related to change of deep supercoolingcapability in xylem parenchyma cells of trees. In “Plant Cold Hardiness -From the laboratory
to the field-”, Gusta L.V., Wisniewski M.E., Tanino K.K. (eds), CAB International, UK, pp 29-42(2009)
Arakawa K., Inada H., Fujikawa S.: Impact of simulated acid snow stress on leaves of cold-acclimatedwinter wheat. In “Plant Cold Hardiness -From the laboratory to the field-”, Gusta L.V.,
Wisniewski M.E., Tanino K.K. (eds), CAB International, UK, pp 173-182 (2009)
Tsutsui, T., Kato, W., Asada, Y., Sako, K., Sato, T., Sonoda, Y., Kidokoro, S., Yamaguchi-Shinozaki, K.,
Tamaoki, M., Arakawa K., Ichikawa, T., Nakazawa, M., Seki, M., Shinozaki, K., Matsui, M., Ikeda, A.,
Yamaguchi, J.: DEAR1, a transcriptional repressor of DREB protein that mediates plant defense
and freezing stress responses in Arabidopsis. Journal of Plant Research, 122: 633-643 (2009)
Umebayashi T., Utsumi Y., Koga S., Inoue S., Fujikawa S., Arakawa K., Matsumura J., Oda K.:
Conducting pathways in north temperate deciduous broadleaved trees. IAWA Journal, 29:247-263 (2008)
Kami D., Kasuga J., Arakawa K., Fujikawa S.: Improved cryopreservation by diluted vitrification
solution with supercooling-facilitating flavonol glycoside. Cryobiology, 57: 242-245 (2008)
Kasuga J., Hashidoko Y., Nishioka A., Yoshiba M., Arakawa K., Fujikawa S.: Deep supercooling xylem
parenchyma cells of katsura tree (Cercidiphyllum japonicum) contain flavonol glycosides
exhibiting high anti-ice nucleation activity. Plant, Cell and Environment, 31: 1335-1348 (2008)

稲田秀俊, 藤川清三, 荒川圭太: 凍結過程に存在する酸性物質が植物細胞の生存に及ぼす影響.
日本冷凍空調学会論文集, 25: 233-238 (2008)
Nagao M., Arakawa K., Takezawa D., Fujikawa S.: Long- and short-term freezing induce different
types of injury in Arabidopsis thaliana leaf cells. Planta, 227: 477-489 (2008)
Takata N., Kasuga J., Takezawa D., Arakawa K., Fujikawa S.: Gene expression associated withincreased supercooling capability in xylem parenchyma cells of larch (Larix kaempferi).
Journal of Experimental Botany, 58: 3731-3742 (2007)
Kasuga J., Arakawa K., Fujikawa S.: High accumulation of soluble sugars in deep supercoolingJapanese white birch xylem parenchyma cells. New Phytologist，174: 569-579 (2007)
Kasuga J., Mizuno K., Arakawa K., Fujikawa S.: Anti-ice nucleation activity in xylem extracts fromtrees that contain deep supercooling xylem parenchyma cells. Cryobiology, 55: 305-314(2007)
Kasuga J., Takata N., Yamane K., Kuroda K., Arakawa K., Fujikawa S.: Larch (Larix kaempferi) xylem
parenchyma cells respond to subfreezing temperature by deep supercooling. CryoLetters, 28:
77-81 (2007)
Umebayashi T., Utsumi Y., Koga S., Inoue S., Shiiba Y., Arakawa K., Matsumura J., Oda K.: Optimalconditions for visualizing water-conducting pathways in a living tree by the dye injection
method. Tree Physiology, 27: 993-999 (2007)
Inada H., Fujikawa S., Saito H., Arakawa K.: Effects of light condition after simulated acid snow stresson leaves of winter wheat. Environmental Sciences, 14, 53-71 (2007)
稲田秀俊, 藤川清三, 荒川圭太: 酸性雪ストレスに対する越冬性植物の生理応答， 低温生物工学会誌. 52: 163-168 (2006)
Nagao, M., Oku K., Minami A., Mizuno K., Sakurai M., Arakawa K., Fujikawa S. and Takezawa D.:
Accumulation of theanderose in association with development of freezing tolerance in the moss
Physcomitrella patens. Phytochemistry, 67: 702-709 (2006)
Inada, H., Nagao, M., Fujikawa, S., Arakawa K.: Influence of simulated acid snow stress on leaf tissueof wintering herbaceous plants. Plant and Cell Physiology, 47: 504-512 (2006)
Arakawa K., Kasuga, J., Takashima, H., Fujikawa, S.: Physiological changes in relation to thedevelopment of freezing resistance in xylem tissue of Betula platyphylla during seasonal cold
acclimation. In “Tree Sap III”, Terazawa,M. (ed), Hokkaido University Press, Sapporo, p.93-97(2006)
Minami, A., Nagao, M., Arakawa K., Fujikawa, S., Takezawa, D.: Physiological and morphologicalalterations associated with development of freezing tolerance in the moss Physcomitrella
patens. In “Cold Hardiness in Plants”, Chen, T.H.H., Uemura, M., Fujikawa, S. (eds), CAB
International, UK, p.138-152 (2006)
Fujikawa, S., Ukaji, N., Nagao, M., Yamane, K., Takezawa, D., Arakawa K.: Functional role ofwinter-accumulating proteins from mulberry tree in adaptation to winter-induced stresses. In“Cold Hardiness in Plants”, Chen, T.H.H., Uemura, M., Fujikawa, S. (eds), CAB International, UK,p.181-202 (2006)
Minami, A., Nagao, M., Ikegami, K., Koshiba, T., Arakawa K., Fujikawa, S., Takezawa, D.: Coldacclimation in bryophytes: low-temperature-induced freezing tolerance in Physcomitrella
patens is associated with increases in expression levels of stress-related genes but not withincrease in level of endogenous abscisic acid. Planta, 220: 414-423 (2005)
Inada, H., Fujikawa, S., Arakawa K.: Influence of the freeze-thaw process under acid conditions onthe viability of wintering plants. Cryobiology and Cryotechnology, 51: 115-119 (2005)
Takahashi, H., Arakawa K., Fujikawa, S.: Study on proteins related to deep supercooling ability ofxylem tissues of Fagus crenata L. Cryobiology and Cryotechnology, 51: 105-109 (2005)
水野薫，春日純，荒川圭太，藤川清三: 針葉樹木部からの氷核形成阻害物質の検出. 低温生物工学会誌, 51: 111-114 (2005)

Nagao, M., Minami, A., Arakawa K., Fujikawa, S., Takezawa, D.: Rapid degradation of starch inchloroplasts and concomitant accumulation of soluble sugars associated with ABA-inducedfreezing tolerance in the moss Physcomitrella patens. Journal of Plant Physiology, 162:
169-180 (2004)
Ukaji, N., Kuwabara, C., Takezawa, D., Arakawa K., Fujikawa, S.: Accumulation ofpathogenesis-related (PR) 10/Bet v 1 protein homologues in mulberry (Morus bombycis Koidz.)
tree during winter. Plant, Cell and Environment, 27: 1112-1121 (2004)
Arakawa K., Hanazaki, M., Yoshida, S.: Frost-susceptible protein in plasma membranes in tubers ofHelianthus tuberosus L. Bioscience, Biotechnology and Biochemistry, 68: 175-182 (2004)
Fujibe, T., Saji, H., Arakawa K., Yabe, N., Takeuchi, Y., Yamamoto, K.T.: A methyl viologen-resistantmutant of Arabidopsis, which is allelic to ozone-sensitive rcd1, is tolerant to supplemental
ultraviolet-B irradiation. Plant Physiology, 134: 275-285 (2004)
Mimami, A., Nagao, M., Arakawa K., Fujikawa, S., Takezawa, D.: Abscisic acid-induced freezing
tolerance in the moss Physcomitrella patens is accompanied by increased expression ofstress-related genes. Journal of Plant Physiology, 160: 475-483 (2003)
Kuroda, K., Kasuga, J., Arakawa K., Fujikawa, S.: Xylem ray parenchyma cells in boreal hardwood
species respond to subfreezing temperatures by deep supercooling that is accompanied byincomplete desiccation. Plant Physiology, 131: 736-744 (2003)
Arakawa K., Kasuga, J., Takashima, H. and Fujikawa, S.: Accumulation of extracellular proteins inbirch xylem during seasonal cold acclimation. Cryobiology and Cryotechnology, 49: 195-201(2003)
田中聡子, 長尾学, 船田良, 藤川清三, 荒川圭太：樹木木部柔細胞の深過冷却の原因と考えられて い る 細 胞 壁 マ イ ク ロ キ ャ ピ ラ リ ー の サ イ ズ を 解 析 す る 試 み . 低 温 生 物 工 学 会 誌 , 49:
209-213 (2003)
春日純, 藤川清三, 荒川圭太：シラカンバ木部組織に蓄積する可溶性糖の季節変化. 低温生物工学会誌, 49: 185-189 (2003)
高田直樹, 竹澤大輔, 南杏鶴, 荒川圭太, 藤川清三：深過冷却で寒冷環境に適応するカラマツ木部柔細胞の遺伝子発現. 低温生物工学会誌, 49: 191-194 (2003)
南杏鶴, 長尾学, 荒川圭太, 藤川清三, 竹澤大輔：アブシジン酸による耐凍性上昇に伴うヒメツリガネゴケの生理的変化の解析. 低温生物工学会誌, 49: 179-183 (2003)
荒川圭太, 稲田秀俊：キクイモ塊茎の凍結傷害過程で生じる細胞膜蛋白質の組成変化に関する研究. 低温生物工学会誌, 49: 203-207 (2003)
Yamada, T., Kuroda, K., Jitsuyama, Y., Takezawa, D., Arakawa K., Fujikawa, S.: Roles of the plasma
membrane and cell wall in the responses of plant cells to freezing. Planta, 215: 770-778(2002)
Kuwabara, C., Takezawa, D., Hamada, T., Shimada, T., Fujikawa, S., Arakawa K.: Abscisic acid andcold-induced thaumatin-like protein in winter wheat has an antifungal activity against snow
mould, Microdochium nivale. Physiologia Plantarum, 115: 101-110 (2002)
Ukaji, N., Kuwabara, C., Takezawa, D., Arakawa K., Fujikawa, S.: Cold acclimation-induced WAP27
localized in endoplasmic reticulum in cortical parenchyma cells of mulberry tree washomologous to group 3 late-embryogenesis abundant proteins. Plant Physiology, 126:
1588-1595 (2001)
Kawamura, Y., Arakawa K., Maeshima, M., Yoshida, S.: ATP analogue binding to the A subunit induces
conformational changes in the E subunit that involves a disulfide bond formation in plant
V-ATPase. European Journal of Biochemistry, 268: 2801-2809 (2001)
Kawamura,Y., Arakawa K., Maeshima, M., Yoshida, S.: Tissue specificity of E subunit isoforms of plantvacuolar H+-ATPase and existence of isotype enzymes. Journal of Biological Chemistry, 275:
6515-6522 (2000)
Fujikawa, S., Kubota, M., Kuroda, K., Takezawa, D., Arakawa K.: Freezing adaptation mechanisms ofliving tissue cells in Betula platyphylla var. japonica Hara and their relation to cell wall
properties. In “Tree Sap II”, Terazawa, M. (ed.), Hokkaido University Press, Sapporo, p.93-96(2000)
Nagao, M., Arakawa K., Takezawa, D., Yoshida, S., Fujikawa, S.: Akinete formation in Tribonema
bombycinum Derbes et solier (Xanthophyceae) in relation to freezing tolerance. Journal ofPlant Research, 112: 163-174 (1999)
久保田勝利, 荒川圭太, 黒田克史, 藤川清三： 低温馴化によるシラカンバ木部放射柔細胞の細胞壁の変化. 低温生物工学会誌. 45: 47-50 (1999)
Ukaji, N., Kuwabara, C., Takezawa, D., Arakawa K., Yoshida, S. and Fujikawa, S.: Accumulation ofsmall-heat shock protein homologs in the endoplasmic reticulum of cortical parenchyma cells inmulberry in association with seasonal cold acclimation. Plant Physiology, 120: 481-489 (1999)
Kuwabara, C., Arakawa K., Yoshida, S.: Abscisic acid-induced secretory proteins insuspension-cultured cells of winter wheat. Plant and Cell Physiology, 40: 184-191 (1999)
Hotsubo, K., Kawamura, Y., Takezawa, D., Arakawa K., Yoshida, S.: Characterization of vacuolar
H+-ATPases that are sensitive and tolerant to cold. In “Cold Hardiness in Plants”, Li, P.H.,
Chen, T. (eds), Plenum Press, USA, p.237-244 (1998)
Koike, M., Takezawa, D., Arakawa K., Yoshida, S.: Accumulation of 19-kDa plasma membrane
polypeptide during induction of freezing tolerance in wheat suspension-cultured cells byabscisic acid. Plant and Cell Physiology, 38: 706-716 (1997)
Odaira, M., Arakawa K., Yoshida, S., Maeshima, M.: Properties of Two N-linked glycoproteinsassociated with the nuclear envelope in mung bean hypocotyls. Plant and Cell Physiology, 36:945-953 (1995)
Kishitani, S., Watanabe, K., Yasuda, S., Arakawa K., Takabe,T.: Accumulation of glycinebetaine duringcold acclimation and freezing tolerance in leaves of winter and spring barley plants. Plant, Celland Environment, 17: 89-95 (1994)Zhou, B.-L., Arakawa K., Fujikawa, S., Yoshida, S.: Cold-induced alterations in plasma membraneproteins that are specifically related to the development of freezing tolerance in cold-hardy
winter wheat. Plant and Cell Physiology, 35: 175-182 (1994)
２）その他の論文（総説、解説）藤川清三，春日純，荒川圭太: 樹木の凍結抵抗性 -凍らない水-． 低温生物工学会誌, 55:
37-41 (2009)
藤川清三, 春日純, 荒川圭太: 凍らない水 ‒深過冷却する樹木木部柔細胞から水を凍結させない物質を探す-. 化学と生物, 43: 280-282 (2005)
藤川清三, 荒川圭太: 植物細胞の深過冷却現象およびそのメカニズム. 低温生物工学会誌, 50:
33-36 (2004)
荒川圭太: 越冬性植物の耐凍性機構. 北海道芝草研究会報, 28: 3-11 (2004)
荒川圭太: 厳しい冬を乗り越える植物 -植物の耐凍性について-. 東北大学遺伝生態研究センター通信, p 4-5 (1995).
(その他)
荒川圭太，藤川清三：第３章 ３．環境ストレスと形成層活動， 木質の構造（日本木材学会編），文永堂出版，p.134-139 （2011）
遠藤圭太, 荒川圭太, 藤川清三：トドマツ針葉の耐凍性および凍結挙動, 日本木材学会北海道支部講演集, 41, 7-10 (2009)
遠藤圭太, 荒川圭太, 藤川清三：カラマツ冬芽（長枝芽）の凍結挙動, 日本木材学会北海道支部
講演集, 40, 11-14 (2008)
葭葉恵，春日純，荒川圭太，藤川清三：木部柔細胞の深過冷却能に与える可溶性糖の効果の検証,
日本木材学会北海道支部講演集, 39, 19-22 (2007)

春日純，葭葉恵，橋床泰之，荒川圭太，藤川清三：カツラ木部柔細胞の深過冷却機構に関与する過冷却促進成分の検索, 日本木材学会北海道支部講演集, 39, 15-18 (2007)
森本和成，藤川清三，荒川圭太：カラマツ木部柔細胞の過冷却能力の変化に伴って変動する蛋白質の検索, 日本木材学会北海道支部講演集, 39, 11-14 (2007)
水野薫，春日純，荒川圭太，藤川清三: カラマツ木部粗抽出画分からの氷核形成阻害活性の検出,
日本木材学会北海道支部講演集, 37: 13-16 (2005)
山根健一, 伊藤利章, 佐野雄三, 荒川圭太, 藤川清三: FE-SEM による急速凍結固定した樹木組織柔細胞の微細構造の観察, 日本木材学会北海道支部講演集, 36: 23-26 (2004)
古俣寛隆, 伊藤利章, 佐野雄三, 荒川圭太, 藤川清三: シロイヌナズ由来の転写制御因子 CBF1を導入したハイブリッドアスペンの耐凍性及び組織構造に関する研究, 日本木材学会北海道支部講演集, 36: 27-30 (2004)
高島寛, 荒川圭太, 藤川清三: 深過冷却するシラカンバ木部柔細胞に蓄積する冬季誘導性蛋白質の機能解析, 日本木材学会北海道支部講演集, 36: 31-34 (2004)
高田直樹, 荒川圭太，南杏鶴，竹澤大輔, 藤川清三: 深過冷却で寒冷環境に適応するカラマツ木部柔細胞において冬季特異的に増加する遺伝子とその組織的発現様式, 日本木材学会北海道支部講演集, 35: 1-4 (2003)
３）特許出願Fujikawa S., Kasuga J., Hashidoko Y., Arakawa K., Fukushi Y.: Supercooling promoting agents. （公開番号：WO2008/007684, 出願：2007年7月, 公開：2008年1月）