Anti-DNA polymerase η antibody, mouse monoclonal (5H10)
Product code70-071Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

Recombinant human alpha Tubulin (full-size)

Isotype

Mouse IgG1κ

Reactivity

Human Pol η protein. Not tested in other species

Special notes

N/A

Application

1. Western blot. (1/500~1/2,000 dilution)
2. Immunofluorescence staining (1/200~1/1,000 dilution)
Not tested for other application

BackgroundDNA polymerase eta ( 713 aa, 78 kDa) is specifically involved in DNA repair. Plays
an important role in translesion synthesis, where the normal high fidelity DNA
polymerases cannot proceed and DNA synthesis stalls. Plays an important role in
the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts
the correct base, but causes frequent base transitions and transversions. May playa role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI(Pol iota) to replication foci.
Involvementin

disease:Xeroderma

pigmentosum

varianttype(XPV)[MIM:278750]: An autosomal recessive pigmentary skin disorder characterized bysolar hypersensitivity of the skin, high predisposition for developing cancers on
areas exposed to sunlight and, in some cases, neurological abnormalities. XPV
shows normal nucleotide excision repair, but an exaggerated delay in recovery ofreplicative DNA synthesis. Most patients with the variant type of xeroderma
pigmentosum do not develop clinical symptoms and skin neoplasias until a later age.
Clinical manifestations are limited to photo-induced deterioration of the skin andeyes. Key Words: DNA polymerase eta, POLH, Y-family DNA polymerase, Xerderma
pigmentosum variant type (XPV), Translesion DNA synthesis, Error-prone DNApolymerase, UV-sensitive, DNA damage tolerance, Skin cancer

Data Link

UniProtKB: Q9Y253 Human Entrez Gene: 5429 HumanPlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 70-071 Anti-DNA polymerase η antibody, mouse monoclonal (5H10)

Fig. 1. Identification of Pol eta in whole cell extract of HeLa cells by western blot with anti-Pol eta
antibody (5H10).
Lane 1; Recombinant full-size Pol eta with His6 tag at C-teminus. Positive control.
Lnae 2; Whole cell extract of HeLa cells. ~40 ug protein applied. Positive control
Lane 3; Whole cell extract of XP2SA (XP-V) cells. ~40 ug protein applied. Negative control
PoL eta is detected at ~80 kDa position. 8% gel was used.
Data by courtesy of Prof. F. Hanaoka and Prof. C. Masutani at Osaka University
Anti-Pol eta antibody

DAPI

MergedFig.2. Immunofluorescence staining of DNA polymerase eta in HeLa cells with anti Pol eta
antibody.
HeLa cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in
PBS for 10 min. anti-Pol eta antibody was used at 1/1,000 dilution.
Reference: This antibody has not been cited in publication yet.
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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/