Anti-SARS-CoV-2 S (Spike) protein antibody, mouse monoclonal (E4)
Product code65-102

Size

50 µg

Storage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

RBD (receptor-binding domain) within SARS-CoV-2 Spike protein

Isotype

Mouse IgG1κ

Reactivity

Receptor Binding Domain of SARS-CoV-2 Spike protein.
Neutralizing activity is synergistic with a monoclonal antibody G2 clone.

Special notes

N/AApplication

1. Western blotting (1-10 μg/ml).
2. Immunofluorescence staining (10 µg/ml)
3. Neutralizing antibody assay (as a positive control).

BackgroundNew coronavirus (SARS-CoV-2) suddenly emerged in Wuhan City, China, atthe end of 2019. SARS-CoV-2 gradually became attenuated in the process oftransitioning to alpha strain, delta strain, and more recent mutant strains
called BA.2, BA.5, BA.2.75. Currently, Omicron strain vaccines developed byModena and Pfizer use mRNA derived from BA.2 viruses, but most of theprevalent virus strains are now replaced by BA.5 and BA.2.75 mutant strains.
This mouse monoclonal antibody (MAbs) was developed as a tool for researchof the mutant strains in which SARS-CoV-2 gradually changes. The S-proteinof SARS-CoV-2 is cleaved into S1 and S2 subunits containing RBD regions by
subtilisin-like protease Furin in host cells. Epitope-recognizing MAb E4 derived
from RBD regions in S1 subunit of the S-protein recognize neutralizing epitope.
It reacts to the same extent as any of the previously predominant SARS-CoV-2mutants. Neutralizing activity is strongly with E4 MAb and weak with G2 MAb.
An interesting point is that significant synergism is found in the presence ofboth MAbs (1:1).

Data Link

N/A

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.

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Data Images: 65-102 Anti-SARS-CoV-2 S (Spike) protein-antibody, mouse monoclonal (E4)

Fig.1. Identification of SARS-CoV-2 S Protein by Western Blotting with anti-SARS-Cov2 S monoclonal
antibody (E4).
Samples: Culture supernatants of SARS-CoV-2 infected Vero/TMPRSS2 cells.
(a)Molecular weight marker proteins(b)

Wuhan strain

(c)

BA.5

(d)

BA.2(e)BA.2.75

Note that this antibody reacted all strains so far tested.
Antibody concentration: 10 μg/ml

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(a)

(b)

(c)

(d)

Fig.2. Staining of SARS-CoV-2 S protein in the virus-infected cells (Vero/TMPRSS2 )by indirect immunostaining with anti-Spike monoclonal antibody, E4.
(a) Wuhan strain; (b) BA.2; (c) BA.2.75; (d) BA.5
Fixed with 4% HCHO/PBS
Antibody concentration: 10 μg/ml.
Note that all strains were reacted with this monoclonal antibody.

Fig.3. The binding inhibition activities of monoclonal antibodies. Inhibition of binding activities ofCoV-2 RBD to ACE2 (SARS-CoV-2 receptor molecules) by monoclonal antibodies, E4, G2 and E4+G2
was measured by using Detection Kit (MBL, CODE 5360).
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Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/Related Products:
65-103 Anti-SARS-CoV-2 S (Spike) protein-antibody, mouse monoclonal (G2)References: This antibody has not yet been used in publication.

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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/