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angiogenic cell-based assay in cancer patients and its application for anti-angiogenic therapy]. Although several efforts havebeen made to develop therapeutic modalities to inhibit angiogenesis, currently available anti-angiogenic therapies are not always
effective, and development of new therapies against angiogenesis is urgent. Previously, we demonstrated that spleen cells inmice stimulated with lipopolysaccharide contain angiogenic cells that can form new blood vessels, and thus, this assay has beendeveloped as a novel circulating angiogenic cell (CAC)-based assay. Human CACs were prepared by the same method as thatused in mice, and their proliferative capacity was examined in 3,3'-diaminobenzidine (DAB)-stained rat aortic rings cultured in
the presence of conditioned medium from human breast cancer cell lines. Conditioned media from breast cancer cell lines
stimulated the proliferation of CACs derived from human peripheral blood. This assay is more sensitive and has a higher
specificity than the conventional tube-based CAC assay. However, when human CACs were cultured with conditioned medium
from human breast cancer cell lines in the presence of the drugs, there was no effect on cell proliferation, even if the tumor
growth-promoting factors in conditioned medium were blocked. This is the first report of an experimental system to evaluate
the effect of drugs on angiogenic factors, and these results suggest that the CAC-based assay is suitable for studies of tumor
angiogenesis, including anti-angiogenic therapy.Q: Qt - How to wait the windows user choos a file and do something? I want tomake my app, which takes a file from the user and then do something with it. Now, I'm trying to use this code: void
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