Anti-Ppa2 (S. pombe) antibody, rabbit serum
Product code63-135Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.05% sodium azidePurity

Rabbit antiserum

Immunogen

Recombinant C-terminal polypeptide (26kDa) of S. pombe Ppa2 (Ref. 1)

IsotypeRabbit IgG

Reactivity

The antibody recognized both Ppa1 and Ppa2 polypeptides in S. pombe becauseof their high amino acid similarity (~80% identity) (Fig.1 and ref. 1).

Special notes

N/A

Application

1. Immunoblotting (dilution: 1/1000)
2. Immunofluorescence microscopy
3. Immunoprecipitation

Background

Schizosaccharomyces pombe Ppa2 is a type 2A-like serine/threonine-protein
phosphatase catalytic subunit whose polypeptide sequence has ~80% identity tothose of mammalian type 2A phosphatases. Ppa2 determines the sensitivity tookadaic acid, which is an inhibitor of protein serine/threonine phosphatases.
The loss of the ppa2 gene causes cells to be hypersensitive to the okadaic acid.
Ppa2 plays important roles in cell cycle control. It may be involved in controllingthe entry into mitosis, possibly acting as an inhibitor (ref.1). Ppa2 is abundantin the cytoplasm, in contrast to the type 1-like phosphatase Dis2, which isenriched in the nucleus. Thus Ppa2 may perform major functions outside thenucleus.

Data Link

UniProtKB P23636 (PP2A2_SCHPO)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 63-135 Anti-Ppa2 (S. pombe) antibody, rabbit serum

Fig.1 Identification of Ppa1 and Ppa2 proteins. An immunoblot with anti-ppa2 antibody is shown(ref.1).
lane 1: Wild-type S. pombelane 2: Δppa1
lane 3: Δppa2
lane 4: Wild-type carrying a multicopy plasmid with ppa1 genelane 5: Wild-type carrying a multicopy plasmid with ppa2 genelane 6:Wild-type carrying a multicopy plasmid with ADH promoter ligated with ppa2 gene
The positions of ppa1 (36 kDa) and ppa2 (39 kDa) polypeptide bands are indicated.

Fig.2 Cellular location of Ppa1 and Ppa2 (ref.1).
Indirect immunofluorescence microscopy of Δppa2 deletion, wild type (WT), and wild-typeoverexpressing ppa2 (nmt-ppa2, 18hr) was done, using anti-ppa2 antibody (right); The same cells
stained by DAPI are also shown (left).
Immunofluorescence was hardly detected in Δppa2 cells, whereas cytoplasmic immunofluorescence
was abundant in wild-type cells. Wild-type cells carrying nmt-ppa2 plasmid overexpress Ppa2 proteinin the absence of thiamine for 18 hr. Immunofluorescence was enhanced further in the cytoplasm,
often accumulated at the nuclear periphery or within restricted domains. The deformation of2 / 3

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chromosomal DNA was also visible in overexpressed cells. Bar, 10um.
References: The antibody has been used in Ref. 1 nad 2.
1. Kinoshita N et al “Negative regulation of mitosis by the fission yeast protein phosphatase ppa2.”

Genes Dev 7: 1059-1071 (1993) PMID: 8389306
2. Kinoshita K et al “The regulatory subunits of fission yeast protein phosphatase 2A (PP2A) affectcell morphogenesis, cell wall synthesis and cytokinesis.” Genes Cell 1:29-45 (1996) PMID: 9078365

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