Technical Briefing Material

Silkworm cell-free protein synthesis system11

Company Profile
Our Mission:
We change the World with our original
silkworm cell-free protein synthesis system!
Trade nameSilk Renaissance Co. Ltd.
(A venture company from Okinawa National College of Technology)

FoundedJanuary 26, 2018

Representative

Representative Director Dr. Masaaki Ito

Headquarters location

Zip code 904-2234
5-8 Suzaki, Uruma, Okinawa, Japan
Okinawa Life Science Research Center 116

Capital stock

24,450,000 JPY (Capital reserve: 21,450,000 JPY)

Accounting periodDecember

Main businesses

Protein Manufacturing Business
Regenerative Medicine Support Business22

About Cell-free protein synthesis system
Technology for synthesizing target proteins in vitro using cell extracts
Live cells are
not used directly

Gene encoding
the target protein

CellExtraction ofcomponents necessary
for protein synthesis

Ribosome, translation factor

Amino acids,
energy source,etc.

cell extracts

Advantages over conventional cell systems:- No need for cell culture- Easier high-throughput- High-throughput synthesis of cytotoxic proteins is possible.
- Easy introduction of non-natural amino acids- Not Recombinant DNA Experiments

DNAin vitro transcriptionreaction

mRNA

Target proteins
Used for synthesis and analysis
of various proteins in basic and沖縄工業高等専門学校
appliedresearch33

Silkworm cell-free protein synthesis system
Silkworm larvalsilk glands

Ribosome, translation factor

Cell extracts preparedfrom silkworm larvalsilk glands

Gene encoding
the target protein

Amino acids,
energy source,etc.

DNAin vitro transcription
reactionmRNA

Target proteins
We have developed an original cell-free protein synthesis systemusing cell extracts of silkworm larval silk glands with high efficiency(>70 μg/ml)
44Feature 1: High protein synthesis among the cell-freeprotein synthesis systems.
Name of Technology

Synthetic amount (catalog value)(μg/ml)Our silkworm cell-free protein synthesis
systemOver 70

Cell-free protein synthesis system using
cultured insect cells(Transdirect insect cell)
Rabbit reticulocyte cell-free translation
system30 - 50

1-4

Silkworm cell-free protein synthesis system:
More than twice as much protein synthesis as the previously
developed product (Transdirect insect cell)55

Comparison with other cell-free protein synthesis systems
rabbit reticulum
red blood cell

Wheatembryo bud

E. coli(S30 fraction)

E. coli(Reconstituted)

silkwormcell-free system

quantityLowHighHighMiddleHighdisassembly

LittleLittleMuch

NoLittle

activity

HighMiddle

Restricted

Restricted

High

Introduction ofnonnatural amino acids

Possible

Possible

Possible

Possible

Possible

impurity

Much

LittleLittleNo

Littlesynthesized protein

Origin of
the extract

Silkworm cell-free protein synthesis system
can produce active protein with little impurity !
66

Feature 2: Solubilized proteins are obtained.
Confirmation of protein synthesis and solubilization by Western blot
analysis using supernatant samples

Silkworm cell-free protein synthesis system:
Capable of highly efficient synthesis of disease-related
proteins in soluble form.

77

Advantages of silkworm cell-free protein synthesis systemcompared to conventional cell-based protein synthesis systems✓ Synthesis is completed in a short time (5 hours)✓ No cell culture required✓ Multiple samples can be processed.
✓ Protein synthesis with cytotoxicity is possible.
✓ Easy introduction of non-natural amino acids✓ Not Recombinant DNA Experiments
Silkworm cell-free protein synthesis system:
Extremely useful technology for rapid protein synthesis !
88Thank you for your attention !
Please consider using our technology to promote
your company's R&D activities.

Contact us !
https://www.silk-r.jp/
99