Symposium Schedule………………………..November 27 (TUE)………………………..
9:00 - 9:05

Opening Remark
KAMBARA, Hideki (Hitachi Ltd., Japan)

Session 1: Imaging Technology and MEMS
Chairperson: KAMBARA, Hideki
9:05 - 9:30

“Atto-Little Chromatography Using Extended-Nano Channel Fabricatedon a Glass Microchip”
KITAMORI, Takehiko (The University of Tokyo, Japan)

9:30 - 9:55

“Evolutionary Bioimaging with Super-Duper Luminescent Proteins”
NAGAI, Takeharu (Osaka University, Japan)

9:55-10:15“Single Cell Based Devices for Analyzing Cellular Signals”
TAMIYA, Eiichi (Osaka University, Japan)

10:15-10:35“Bio-Imaging for Detection Of Cell Surface Carbohydrates andIntracellular microRNA”
JU, Huangxian (Nanjing University, China)

10:35-10:50

Break (15 min)Introduction of Analytical Tools from Industrial Fields
Chairperson: UEDA, Mitsuyoshi
10:50-11:00“Fluidigm Biomark Single-Cell Gene Expression”
JONES, Bob (Fluidigm, USA)

11:00-11:10“Multiplexed Single Cell Microarrays”
NETTIKADAN, Saju (NanoInk, Inc., USA)

11:10-11:20“Genome Analysis of Individual Cells”
KORFHAGE, Christian (QIAGEN GmbH, Germany)

11:20-11:30“Quantitative Introduction of Bio-Molecular Substances into Cultured
Cells Using Automated Microinjector (CI-2500)”

CHUNG, Jihye (1 Fujitsu Ltd., 2 Waken B Tech Co. Ltd., Japan)
11:30-11:50

Break (20 min)

11:50-12:50

Poster Short Presentation 1Chairperson: ARAKAKI, Atsushi

12:50-14:20

Poster Presentation 1 with Lunch

Session 2: Molecular Tools for Single-Cell Analysis
Chairperson: MATOBA, Ryo
14:20-14:45“Not Your Obaasan’S (䛚፠䛥䜣’s) GFP: New Tools for Imaging andControlling Interactions in Cells”
BOXER, Steven (Stanford University, USA)14:45-15:05“Quantitative Comparison of Protein Dynamics in Live Cells and in vitro
by In-Cell 19F-NMR”
HAMACHI, Itaru (Kyoto University, Japan)

15:05-15:25“Functional Characterization of Single Cells”
SÖDERBERG, Ola (Uppsala University, Sweden)

15:25-15:45“Novel Stigmatic Mass Microscope Using Laser Ionization”
HAZAMA, Hisanao (Osaka University, Japan)

15:45-16:05“What Do FRET Biosensors Show in Single Living Cells?”
MATSUDA, Michiyuki (Kyoto University, Japan)

16:05-16:25

Break (20 min)General Session
Chairperson: HAMACHI, Itaru
16:25-16:37“Analysis of Protein and Protein Complexes via Proximity Ligation”
WU, Di (Uppsala University, Sweden)

16:37-16:49“Microcavity Array System for Isolation of Circulating Tumor Cells inCancer Patients”
HOSOKAWA, Masahito (Shizuoka Cancer Center Research Institute, Japan)

16:49-17:01“From Single Cell Type to Single-Cell Transcriptome with nanoCAGE”
PLESSY, Charles (RIKEN Omics Science Center)

17:01-17:13“Initialization and Cell Cycle Study of Single Cells Using Micro-OrificeElectroporation Device”
OKEYO, Kennedy (The University of Tokyo, Japan)

17:13-17:25“Standardization of Human iPS Cells with Single Cell Technology”
TANAKA, Michihiro (Kyoto University, Japan)

17:25-17:37“Cross-Omics Studies on Gene Regulation Networks of K562 Cell”
LU, Zuhong (Southeast University)

18:00-20:00

Evening Chat

………………………..November 28 (WED)………………………..

Session 3: Omics
Chairperson: TAMIYA, Eiichi
9:00 - 9:25

“Gene Expression Analysis at Single-Cell Resolution”
SHIRAI, Masataka (Hitachi Ltd., Japan)

9:25 - 9:50

“Single-Cell Biosignatures”
MELDRUM, Deirdre (Arizona State University, USA)

9:50-10:10“Development of Microbial Cell Factories for The Production of Bio-Fuels and
Bio-Based Chemicals Based on Systems Biology Approaches”
KONDO, Akihiko (Kobe University, Japan)

10:10-10:30“Quantitative Analysis of Single-Cell Transcriptomes”
LINNARSSON, Sten (Karolinska Institute, Sweden)

10:30-10:50“Application of Metabolomics to High Resolution Phenotype Analysis”
FUKUSAKI, Eiichiro (Osaka University, Japan)

10:50-11:00

Break (10 min)

11:00-12:00

Poster Short Presentation 2
Chairperson: BAMBA, Takeshi

12:00-13:30

Poster Presentation 2 with Lunch

Session 4: Developmental and Medical Applications
Chairperson: BOXER, Steven
13:30-13:50“Noninvasive Prenatal Detection of Fetal Chromosomal Aneuploidy in China”
HUANG, Huan (Nanjing Medical University, China)

13:50-14:15“Conducting HIV Vaccine Trials in Africa: Update, Opportunities andChallenges”
JAOKO, Walter (University of Nairobi, Kenya)

14:15-14:40“Mechanism and Reconstitution in vitro of Germ Cell Development in Mice”

SAITOU, Mitinori (Kyoto University, Japan)
14:40-15:00“Single Cell Gene Expression Analysis for Hematopoietic Stem Cells”
YAMATO, Masayuki (Tokyo Women’s Medical University, Japan)

15:00-15:10

Poster Award Ceremony
KAMBARA, Hideki (Hitachi Ltd., Japan)

15:10-15:30

Break (20 min)Chairperson: TAKEYAMA, Haruko
15:30-15:55“Single Cell Morphometry in True 3D Using Cell-CT”
NELSON, Alan (Arizona State University, USA)

15:55-16:15“FDD-MB System, the Present and the Future”
TAKABAYASHI,University,

16:15-16:35

2

Haruo1.,

SEKIZAWA,Akihiko2(1Kanazawa

MedicalShowa University School of Medicine, Japan)“NGS of Immune Repertoire: Technologies and Applications”
HAN, Jian (HudsonAlpha Institute for Biotechnology, USA)

16:35-16:55“Ultra-Low Input Amplification-Free Whole-Genome Bisulfite Sequencing: A
Step Toward Single-Cell Methylomics”
ITO, Takashi (The University of Tokyo, Japan)

16:55-17:00

Closing Remark
BOXER, Steven (Stanford University, USA)