Anti-Chickungunya Virus Capsid antibody, mouse monoclonal (CV-901)
Product code

65-420Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified recombinant Chikungunya virus (CHIKV) capsid protein

ImmunogenSynthetic peptide containing phosph-Ser20Isotype

Mouse IgG1κ

Reactivity

Capsid protein of CHIKVSpecial notes

Epitope: Major domain (amino acid 1 to 261) of CHIKV capsid protein.

Application

1. Western blot (1/500~1/1,000)
2. Immunofluorescence staining (1/500)
3. ELISA (assay dependent)
Other applications have not been tested.

Background

Chikungunya virus (CHIKV) is the aetiological agent of chikungunya fever,which is a mosquito-borne pathogen responsible for epidemics of debilitating
arthritic disease. CHIKV belongs to the Alphavirus genus within theTogaviridae family and is an enveloped, single-stranded positive-sense RNAvirus. The alphavirus genome is encodes four non-structural proteins (nsP1 tonsP4) and five structural proteins (capsid, E3, E2, 6K and E1). The alphavirus
capsid protein (CP) is a multifunctional protein that has been shown to act as aserine protease for self-cleavage, bind viral genomic RNA and other CP
molecules during nucleocapsid formation, and interact with viral spike proteinsduring virion formation and egress. The CP of CHIKV consists of 261 aminoacids, which form two major domains. The N- terminal domain has a high degreeof positive charge implicated in non-specific RNA binding, while the C-terminal
domain harbors the globular protease and the binding site for the spike protein.

Data Link

UniProtKB:Q5XXP3(POLS-CHIK3)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 65-420 Anti-Chickungunya Virus Capsid antibody, mouse monoclonal (CV-901)

Fig.1. Identification of protein in CHIKV by Western blotting using monoclonal antibody.
The lysates of recombinant CHIKV protein (0.5mg/ml) were applied to SDS-PAGE. The monoclonal
antibody was used at 1/500 dilution. The HRP-conjugated goat anti-mouse IgG (abcom) was used at
1/4,000 as the second antibody. A 34-35kDa band was identified as CHIKV capsid protein.

Fig.2. Detection of CHIKV capsid protein in CHIKV-infected cells (C6/36) by immunofluorescencestaining. CHIKV infected cells (a) and uninfected cells (b) on a slide glass were fixed with aceton.
The MAb was used at 1/500 dilution. The FITC-conjugated rabbit anti-mouse IgG (abcom) was usedat 1/4,000 as the second antibody.

Fig.3. Titration of protein concentration of CHIKV by indirect ELISA using monoclonal antibody
The indicated amounts of recombinant CHIKV were coated onto the wells of the ELISA plate. Afterblocking with 5% skim milk, monoclonal antibody at the 1/5,000 dilution was added to the each well.
HRP-conjugated goat anti-mouse IgG (100μl, x4,000 dilution, abcom) was added. As substrate,orthophenylenediamine was used. Optical density (OD) measured at 490nm.
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Reference: This antibody has not been used in publication yet.

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BioAcademia,Inc. Tel. 81-6-6877-2335

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