MODEL 2020

Advanced Tomography Holder
A revolutionary holder that allows room
temperature data collection over wide tilt andtranslation ranges, even in restrictive pole-piecegap geometries.

• Ideal for room temperature electron
tomography• High tilt angles
PLASMA CLEANING
Fischione recommends that you clean thespecimen and specimen holder with theFischione Model 1020 Plasma Cleaner orModel 1070 NanoClean before insertion
into the TEM.
During collection of tomographic data,
the electron beam will be on the same
area of the specimen for an extendedtime. As a result, organic contaminationmay build up on the specimen. A plasma
cleaning time of 10 seconds to 2 minutes
removes the contamination. Longer
cleaning times can remove contamination
spots caused by previous TEM viewing ofnon-plasma cleaned specimens.
When not in use, the holders should be
stored under vacuum in Fischione Model
9010 Vacuum Storage Containers or theModel 9020 Vacuum Pumping Station.

• Maximized field of view
• Optimized specimen clamping• Easy, accurate specimen loading andcenteringM O D E L 2020 A d va n c e d To m o gra p hy H o l d e rThree-dimensional information
Advances in microstructural characterization
require the ability to analyze structure andchemistry in three dimensions. However, mostTEM techniques are limited to producing twodimensional information. Tomography, on theother hand, combines two-dimensional data setstaken at various tilt angles to produce threedimensional information.
Biological research has benefited from the use ofelectron tomography for many years; however,the physical sciences have been limited by theinability to tilt the specimen to high angles
within the confinesof the narrowEliminates thegap pole pieces
shadowingnecessary for atomic
associated withresolution imaging.
most holders atIn addition, therehigh-tilt angles
was an increasing
desire to use highangle annular dark field scanning transmission
electron microscopy (HAADF‑STEM) to
reduce diffraction contrast in physical science
applications. Now, Fischione’s advanced specimen
holder technology enables room temperature
tomography for both the life and the physicalsciences.

Maximized field of view at tilt angles
above 70° with no shadowing
The Fischione Model 2020 Advanced
Tomography Holder for transmission electron
microscopes (TEMs) features high tilt andextended field of view. The revolutionary holder
allows room temperature data collection overwide tilt and translation ranges even in restrictivepole-piece-gap geometries.
The Advanced Tomography Holder is for life
sciences and physical sciences, as well as any otherapplications requiring high specimen tilt andsimultaneous large field of view. The Advanced
Tomography Holder’s streamlined specimen

E.A. FISCHIONE INSTRUMENTS, INC.

clamping mechanism eliminates the shadowing
associated with most holders at high‑tilt angles.

Accepts wide range of specimen
thicknesses
The Advanced Tomography Holder’s clamping
mechanism accepts specimen grids, standard
3 mm diameter TEM specimens, or focused ion
beam (FIB) lamella. The Advanced Tomography
Holder accommodates specimen thicknesses up
to 250 µm.

Evenly distributed force from fully
retractable clamp
Specimens are secured with two clamps that
produce an evenly distributed force on opposing
edges of the specimen. For convenience duringloading and unloading, the clamps are springloaded to lift them off the specimen surface.
Then, they can be fully retracted.
Positioning the clamps is done without contactingthe specimen, eliminating the possibility ofspecimen damage. This is far superior to typicalclamping mechanisms that limit the specimen
size or interfere with viewing at high-tilt angles.

Easy, accurate, self-centering
positioning
The tapered self-centering specimen receptacle
guides the specimen into position. The fully
retractable clamps make it easy to rotate thespecimen manually for a dual-axis tilt series.

Touch protection
Fischione’s Advanced Tomography Holders arecompatible with the TEM’s touch alarm, which
stops goniometer movement in the event that apole touch occurs. Always be aware of the TEM’spole piece configuration and follow the microscope
manufacturer’s recommendation for operating thegoniometer at high-tilt angles.

3M O D E L 2020 A d va n c e d To m o gra p hy H o l d e rOrdering information
All Fischione Advanced Tomography Holders
come with a dedicated loading station for secure
specimen handling, tools to assist in specimen
clamping, and a Fischione Model 9010 Vacuum
Storage Container for storing the holder in aclean, vacuum environment.

E.A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632 USA
Tel: +1 724.325.5444
Fax: +1 724.325.5443
info@fischione.com
www.fischione.com©2015 E.A. Fischione Instruments, Inc. All rights reserved.
United States patent number 7,219,565.
Document Number PB2020 Revision 02 07/2015