ポスター Poster
第1 日目（11 月25 日（金））／Day 1（Nov. 25 Fri.）大会議室 101 ＋102、多目的ホール／Conference Room 101+102, Multi-Purpose Hall

蛋白質：構造 / Protein: Structure
1Pos001

Crystal structure of the 11-cis isomer of Pharaonis Halorhodopsin
Siu Kit Chan1, Haruki Kawaguchi1, Hiroki Kubo1, Kunio Ihara3, Kosuke Maki1, Tsutomu Kouyama1,2 (1Grad. Sch. of Sci., Nagoya Univ., 2RIKEN

1Pos002

Harima Branch, 3Center for Gene Research, Nagoya Univ.)
結晶構造から明らかになった、エンドセリン-1 によるエンドセリン受容体 B 型の活性化機構
Crystal structures of the endothelin receptor type B reveal activation mechanism by endothelin-1
Wataru Shihoya1,2, Tomohiro Nishizawa3,4, Akiko Okuta2, Kazutoshi Tani2, Yoshinori Fujiyoshi1,2, Osamu Nureki3, Tomoko Doi5 (1Grad. Sch.

Sci., Nagoya Univ., 2Cellular and Structural Physiology Institute., Nagoya Univ., 3Grad. Sch. Sci., Univ. Tokyo, 4JST PRESTO, 5Grad. Sch. Sci.,
Kyoto Univ.)
1Pos003* X 線結晶構造解析による軸糸ダイニン軽鎖１の構造評価
X-ray crystallographic characterization of the axonemal dynein light chain-1
Akiyuki Toda1, Hideaki Tanaka2, Yosuke Nishikawa2, Toshiki Yagi3, Genji Kurisu2 (1Grad. Sch. Sci., Osaka Univ., 2Institute for Protein Research,
3Facult. Life Environ., Pref. Univ. Hiroshsima)

1Pos004

固定子に作用するべん毛内膜蛋白質 FliL の構造解析
Structural analysis of the Stator Associated Inner Membrane Protein FliL from Vibrio alginolyticus
Miyu Isumi1, Yuuki Nishino2, Mayuko Sakuma2,3, Seiji Kojima2, Michio Homma2, Katsumi Imada1 (1Grad. Sch. of Sci., Osaka Univ., 2Grad. Sch.

1Pos005
1Pos006of Sci., Nagoya Univ., 3Radioisotpe Res. Cent.)
Oligomeric structure of the ExbB-ExbD complex revealed by X-ray crystallography and cryo-EM
Saori Maki-Yonekura, Yoshiki Yamashita, Rei Matsuoka, Maiko Tanaka, Fumie Iwabuki, Koji Yonekura (RIKEN SPring-8 center)
赤痢菌ニードル複合体の極低温電子顕微鏡による構造解析
Structural analysis of needle complex from shigella flexneri by cryo electron microscopy
Naoko Kajimura1,2, Takayuki Kato1, Ariel J Blocker3, Kei-ichi Namba1,4 (1Grad. Sch. of Frontier Biosci., Osaka Univ., 2Res., Center for UHVEM,

Osaka Univ., 3Sch. of Cell. & Mol. Med., Univ. of Bristol, 4RIKEN, QBiC)
1Pos007 単一ミオシン結合状態のアクトミオシンの高分解能化
F-actin structural changes induced by a single myosin head
Takahiro Namise, Kazuaki Yoshida, Takuo Yasunaga (Kyushu Institue of Technology)
1Pos008* NMR analysis of C-terminal periplasmic domain of flagellar motor protein MotB and its active mutant L119P
Gaby Almira1, Ikumi Kawahara1, Seiji Kojima2, Katsumi Imada3, Toshimichi Fujiwara1, Michio Homma2, Chojiro Kojima1,4 (1Inst. for Prot. Res.,

1Pos009

1Pos010

Osaka Univ., 2Div. of Biol. Sci., Grad. Sch. of Sci., Nagoya Univ., 3Dept. of Macromol. Sci., Grad. Sch. of Sci., Osaka Univ., 4Grad. Sch. of Eng.,
Yokohama National Univ.)
CS-Rosetta 法によるヒストン H2A-H2B ヘテロ二量体の溶液構造解析
Determination of the solution structure of isolated histone H2A-H2B heterodimer by using CS-Rosetta
Tsutomu Yamane, Yoshihito Moriwaki, Hideaki Ohtomo, Mitsunori Ikeguchi, Jun-ichi Kurita, Masahiko Sato, Aritaka Nagadoi, Hideaki Shimojo,
Yoshifumi Nishimura (Graduate School of Medical Life Science, Yokohama City University)
時間分解 EPR 法によるヒトインスリンのアミロイド線維化による構造変化の観測
Effects of amyloid fibrillations on geometries of human insulin as studied by time-resolved EPR spectroscopy
Tomoka Abe1, Takashi Tachikawa1, Eri Chatani1, Paul Zierep2, Stefan Weber2, Toshifumi Mori3, Shinji Saito3, Yasuhiro Kobori1 (1Grad. Sch. Sci.,

1Pos011

1Pos012

Kobe Univ., 2Freiburg Univ., 3IMS)
溶液 NMR 法を用いた長距離情報の取得による Nrd1 のドメイン間配向の決定
Structural analysis of a multi-domain protein using long-range distance information derived by solution NMR
Kan Nagai, Ayaho Kobayashi, Yutaka Ito, Masaki Mishima (Graduate School of Science and Engineering, Tokyo Metropolitan University)
サイズ排除クロマトグラフィー／X 線小角散乱法に基づいたニトリラーゼ会合体プロトマーの構造特性
Structural characterization on nitrilase protomers analyzed by size-exclusion chromatography/small-angle X-ray scattering (SEC-SAXS)
Masatoshi Usui1,2, Ryo Ishiguro1,2, Homare Yokota1,2, Yusuke Takeda1,2, Takaaki Hikima2, Tetsuro Fujisawa1,2 (1Grad. Sch. Eng., Gifu Univ.,
2SPring-8 Center, Harima Inst., RIKEN)

1Pos013* 様々な炎症物質を認識する NLRP3-LRR ドメインの構造基盤の解明
Investigation of molecular basis underlying the recognition of various inflammatory substances by NLRP3-LRR domain
1Pos014

Ryota Yamamoto1, Kazuto Yamashita1, Hiroshi Imamura2, Motonari Tsubaki1, Eri Chatani1 (1Grad. Sch. Sci., Univ. Kobe., 2AIST)PRP の匂い分子結合における構造変化
The structural changes of the peri-receptor protein (PRP) on the odorant-binding process
Xing Li1, Durige Wen1, Mitsuhiro Hirai2, Noboru Ohta3, Masaru Hojo4, Mamiko Ozaki4, Tatsuo Iwasa1,5 (1Div. Eng., Muroran Ins. of Tech., 2Dept.
Phys., Gunma Univ., 3JASRI, 4Dept. Biol., Grad. School Sci., Kobe Univ., 5Cen. Env. Sci. Dis. Mit. Adv. Res., Muroran Ins. of Tech.)– S81 –

1Pos015

放射光小角散乱データに基づく対称性を考慮したニトリラーゼオリゴマーのモデリング
Modeling of Nitrilase oligomer with flexible symmetry based on synchrotron small-angle scattering data
Tetsuro Fujisawa1,2,3, Keiichi Kameyama1, Ryo Ishiguro1,2 (1Dep. Chem. & Biomol. Sci., Fac. Eng., Gifu Univ., 2Spring-8 Center, RIKEN Hrima

1Pos016

1Pos017

1Pos018

1Pos019

Inst., 3Synchro. Center, Nagoya Univ.)
β-シート中におけるアミノ酸トリプレットパターンの解析
Analysis of amino acid triplet patterns in β-sheets
Hiromi Suzuki (School of Agri., Meiji Univ.)
タンパク質の構造コンプライアンス特性とドメイン間運動の関係性解析
Analysis of the Relationship Between Structural Compliance Properties and Inter-domain Motion of Proteins
Keisuke Arikawa (Fcl. Eng., Kanagawa Inst. of Tech.)
自由エネルギー変分原理に基づく Pim-1 キナーゼ阻害剤系の相対的結合自由エネルギーの予測
Prediction of the relative binding free energies for Pim-1 kinase - inhibitor systems based on the free energy variational principle
Anna Hirai (Dept. of Bioinfo., Col. Life., Ritsumeikan Univ.)
Flexible docking between cyclin-dependent kinase 2 and its inhibitor using multicanonical MD
Gert-Jan Bekker1, Narutoshi Kamiya2, Mitsugu Araki3, Yasushi Okuno4, Haruki Nakamura1 (1IPR, Osaka Univ., 2Grd. Sch. SS, Univ. Hyogo,
3AICS, RIKEN, 4Grd. Sch. Med., Kyoto Univ.)

1Pos020* 高濃度リガンド条件による蛋白質-リガンド結合部位および経路の効率的探索
Accurate and efficient protein-ligand docking method using all-atom molecular dynamics at high concentration of ligands
1Pos021

Chika Sato1, Akio Kitao1,2 (1Grad. Sch. Front. Sci., Univ. Tokyo, 2IMCB, Univ. Tokyo)
自由エネルギー変分原理を用いたタンパク‐リガンド間相対的結合自由エネルギー計算の DHFR-TMP 系への応用
Calculation of relative binding free energy between DHFR-TMP system on the basis of free energy variational principle
Naoto Nishimura (Grad. Sch. bioinfo., Univ. Ritsumeikan)

蛋白質：構造機能相関 / Protein: Structure & Function
1Pos022

巨大ヘモグロビン酸素解離中間体の X 線結晶構造と分光学的解析
Crystallographic and spectroscopic analysis of the oxygen-dissociation intermediate of the giant hemoglobin
Nobutaka Numoto1, Taro Nakagawa2, Nobutoshi Ito1, Yoshihiro Fukumori3, Kunio Miki4 (1Med. Res. Inst., Tokyo Med. & Dent. Univ., 2Nagahama

1Pos023Inst. of Bio-Sci. & Tech., 3Coll. of Sci. & Eng., Kanazawa Univ., 4Grad. Sch. of Sci., Kyoto Univ.)
Structural analysis of Calredoxin from Chlamydomonas reinhardtii
Ratana Charoenwattanasatien1,2, Risa Mutoh2, Hideaki Tanaka2, Takashi Matsumoto3, Takashi Oda4, Mamoru Sato4, Michael Hippler5, Genji

1Pos024

Kurisu1,2 (1Grad. Sch. Sci., Osaka Univ., 2Inst. Protein Res, Osaka Univ., 3Rigaku, 4Grad. Sch. of Med. Life Sci., Yokohama City Univ., 5Inst. Plant
Biol. and Biotech., Univ. of Munster)
分子動力学による溶液中 Agaricus brasiliensis 由来 β-グルカンの構造解析
Conformational analysis of β-glucans from Agaricus brasiliensis revealed by molecular dynamics in solution
Yoshitaka Matsumura1, Kodai Inoue1, Makoto Suminokura1, Mikako Kubo1, Mariko Demura1, Takayuki Ichioka1, Yasumasa Morimoto1, Mitsuru
Tashiro2, Ken-ichi Ishibashi3, Naohito Ohno3, Masaki Kojima1 (1Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 2Dept. of Chem., Coll. of Sci.

1Pos025
1Pos026

1Pos027

1Pos028

1Pos029

1Pos030

and Tech., Meisei Univ., 3Sch. of Pharm., Tokyo Univ. of Pharm. and Life Sci.)
Aβ conformation on a hydrophilic/hydrophobic interface by molecular dynamics simulations
Satoru Itoh1,2, Hisashi Okumura1,2 (1IMS, 2Sokendai)
分子動力学シミュレーションを用いた四量体型サルコシン酸化酵素における生成物の選択的移動の解明
Selective transport of product in heterotetrameric sarcosine oxidase by molecular dynamics simulation
Go Watanabe1, Takami Saito2, Daisuke Nakajima1, Akinori Hiroshima1, Haruo Suzuki1, Shigetaka Yoneda1 (1Sch. Sci., Kitasato Univ., 2Grad. Sch.
Sci., Kitasato Univ.)
レオニン合成酵素における生成物支援機構の理論的解明
Theoretical elucidation on the product assisted catalysis of threonine synthase
Yuzuru Ujiie1, Mitsuo Shoji1, Ryuhei Harada1, Takeshi Murakawa2, Yasuteru Shigeta1, Hideyuki Hayashi2 (1Univ. of Tsukuba, 2Osaka Medical
College)
Hybrid ab initio molecular dynamical simulation of cytochrome c oxidase: Mechanisms of structural changes by dynamical ligand
recognition
Ryuichiro Terada, Kang Jiyoung, Masaru Tateno (Grad. Sch. Sci., Univ. Hyogo)
スレオニル tRNA 合成酵素におけるアミノ酸選択機構の理論的研究
Theoretical study on the molecular mechanism of amino-acid selection in threonyl-tRNA synthetase
Yoshiharu Mori1, Hisashi Okumura1,2 (1IMS, 2SOKENDAI)
量子化学計算ソフトウェアへのレプリカ交換法の導入
The implementation the Replica-Exchange Umbrella Sampling in the quantum mechanical simulation packages
Shingo Ito1, Yuko Okamoto3, Stephan Irle1,2 (1Department of Chemistry, Graduate School of Science, Nagoya University, 2Institute of

1Pos031Transformative Bio-Molecules (WPI-ITbM), Nagoya University, 3Department of Physics, Graduate School of Science, Nagoya University)
Hydrogenase : ab initio quantum mechanics study of oxygen-tolerance mechanism
Jae Hyun Kim, Jiyoung Kang, Masaru Tateno (Graduate school of Life Science,University of Hyogo)– S82 –

1Pos032* アルカン合成酵素 AD の NMR と分子動力学シミュレーションによるダイナミクス解析
Structural dynamics of an alkae synthase, AD, studied by NMR and molecular dynamics simulations
Yuma Suematsu1, Yuji O. Kamatari2, Yuuki Hayashi1, Munehito Arai1 (1Dept. of Life Sci., Univ. of Tokyo, 2Life Sci. Res. Center, Gifu Univ.)
1Pos033* エンド-1,3-β-グルカナーゼ触媒ドメインの構造ダイナミクス解析
Structural dynamics analysis of catalytic domain of endo-1,3-β-glucanase
Ayako Miki1, Satomi Inaba1, Kazumasa Sakurai2, Masayuki Oda1 (1Grad. Sch. of Life and Environ. Sci., Kyoto Pref. Univ., 2High Pressure Protein
Res. Center, Kindai Univ.)
1Pos034 カルモジュリン結合ペプチドはミッドカインに親和性を示し、その一アミノ酸変異による立体構造変化が結合親和性の向上に寄与する
Secondary structure change by single alanine substitution in Calmodulin-binding peptide improved the binding affinity with Midkine
Hidenao Arai, Koji Matsuoka, Naoto Nemoto (Grad. Sch. Sci. Eng., Saitama Univ.)
1Pos035 ラン藻由来アルカン合成酵素のアラニンスキャン変異解析
Alanine scanning mutagenesis of a cyanobacterial alkane synthase
Keigo Shimba, Fumitaka Yasugi, Yuuki Hayashi, Munehito Arai (Dept. Life Sci., Univ. Tokyo)
1Pos036* 複数のエピトープを認識する G2 の 1 本鎖抗体の構造解析
Structural analysis of a single-chain Fv antibody of G2 that recognizes multiple epitopes

1Pos037

Daiki Usui1, Yuji O. Kamatari2, Satomi Inaba1, Masayuki Oda1 (1Grad. Sch. of Life and Environ. Sci., Kyoto Pref. Univ., 2Life Sci. Res. Ctr., Gifu
Univ.)
複数の抗原を特異的に認識する抗体 G2 の 3 つめのエピトープの同定
Identification of the third epitope recognized by multispecific antibody G2
Md. Nuruddin Mahmud1, Yasuo Inoshima1, Naotaka Ishiguro1, Yuji O. Kamatari2 (1United Grad. Sch. Veterinary Sci., Gifu Univ., 2Life Sci. Res.
Ctr, Gifu Univ.)

1Pos038* ラン藻でのアルカン合成に必要な 2 つの酵素間の結合部位の探索
Search for the binding sites between two enzymes essential for cyanobacterial alkane biosynthesis
Mari Chang1, Keigo Shimba2, Yuuki Hayashi2, Munehito Arai1,2 (1Department of Physics, University of Tokyo, 2Department of Life Sciences,
University of Tokyo)
1Pos039* ケモカイン受容体制御因子 FROUNT－制御化合物間の立体構造情報に基づく相互作用解析
Structure-based analyses of the interaction between the chemokine receptor-regulator FROUNT and anti-inflammatory compounds
Soichiro Ezaki1, Sosuke Yoshinaga1, Norihito Ishida1, Mitsuhiro Takeda1, Kaori Yunoki1, Yuya Terashima2, Etsuko Toda2, Kouji Matsushima2,
1Pos040

Hiroaki Terasawa1 (1Faculty of Life Sciences, Kumamoto University, 2Graduate School of Medicine, The University of Tokyo)
CD28 と SH2 ドメインとの相互作用における構造熱力学的解析
Structural and thermodynamic analysis of interactions between CD28 and SH2 domains
Satomi Inaba1, Nobutaka Numoto2, Shuhei Ogawa3, Hisayuki Morii4, Teikichi Ikura2, Ryo Abe3, Nobutoshi Ito2, Masayuki Oda1 (1Grad. Sch. Life

1Pos041

Environ. Sci., Kyoto Pref. Univ., 2Med. Res. Inst., Tokyo Med. Dent. Univ. (TMDU), 3Res. Inst. Biomed. Sci., Tokyo Univ. Sci., 4College Liberal Arts
Sci., Tokyo Med. Dent. Univ.)
T 細胞受容体による特異的および交差反応的な抗原認識機構の解明
Analyses of the structural mechanisms of specific and crossreactive recognitions of peptide-MHC by TCRs
Yuko Tsuchiya1, Yoshiki Namiuchi2, Hiroshi Wako3, Hiromichi Tsurui4 (1IPR, Osaka Univ., 2QBiC, RIKEN, 3Sch. of Social Sci., Waseda Univ.,
4Sch. of Med., Juntendo Univ.)

1Pos042

1Pos043

1Pos044

1Pos045

1Pos046

1Pos047

レプリカ交換分子動力学シミュレーションによって明らかになった HP1αCD/histone H3 tail 複合体形成の仕組み
Mechanism of the complex formation of HP1αCD/histone H3 tail revealed by the replica-exchange molecular dynamics simulations
Satoshi Omori1, Nobuto Hashiguchi2, Kei Moritsugu2, Yoshifumi Nishimura2, Akinori Kidera2 (1GSIS, Tohoku Univ., 2Grad. Sch. of Med. Life Sci.,
Yokohama City Univ.)
Go-like モデルを用いたプラストシアニンとシトクロム f 複合体の構造安定性に関する理論的研究
Theoretical study on the structural stability of plastocyanin and cytochrome f complex by using Go-like model
Satoshi Nakagawa, Shogo Kinoshita, Makoto Wada, Kazutomo Kawaguchi, Hidemi Nagao (Grad. Sch. Nat. Sci. Tech., Kanazawa Univ.)
粗視化分子動力学シミュレーションを用いた MVM の生物物理学的特性の解明
Elucidating biophysical properties of the Minute Virus of Mice capsid: Coarse-Grained Molecular simulation
Koji Ono, Shoji Takada (Dept. Biophys., Grad. Sch. Sci., Kyoto Univ.)
粗視化力場を用いたタンパク質-リガンド結合シミュレーション：結合経路上の変異がリガンド結合に及ぼす影響の解析
Coarse-grained simulations of protein-ligand binding: effect of mutations near the ligand-binding pathways
Tatsuki Negami, Tohru Terada, Kentaro Shimizu (Grad. Sch. of Agri. and Life. Sci., The Univ. of Tokyo)
タンパク質-リガンドドッキング計算における最適パラメータの同定による分子設計の拡張
Identification of optimal parameter values in ligand-receptor docking calculation to extend applicability
Takuya Sumi1, Hiroshi Yamaguchi2, Ryuichiro Terada1, Jiyoung Kang1, Masaru Tateno1 (1Grad. Sch. Sci., Univ. of Hyogo, 2Grad. Sch. Med.,
Nagoya Univ.)
Analysis of protein complexes structures towards rational design of inhibitors of Protein-protein interactions (PPIs)
Daisuke Kobayashi, George Chikenji (Nagoya Univ.)– S83 –

蛋白質：物性 / Protein: Property
1Pos048* 天然タンパク質の立体構造物性に関する統計解析
Statistical analysis on the structural properties of native proteins
Hidenobu Kawai, Daisuke Takahashi, Munehito Arai (Dept. Life Sci., Univ. Tokyo)
1Pos049 膜貫通 β バレルにおける β ストランドのねじれと曲りに関する解析
Twisting and bending of β-strand in the transmembrane β-barrel
Nobuaki Kikuchi, Shinichi Ebisawa, Yuka Watanabe, Kazuo Fujiwara, Masamichi Ikeguchi (Dept. Bioinfo., Grad. Sch. Eng., Soka Univ.)
1Pos050 球状蛋白質の構造的性質とフォールディング速度との相関
Relationship between the Folding Rate and Structure-based Properties of Globular Proteins
Balachandran Manavalan2,3, Kunihiro Kuwajima1,2, Jooyoung Lee2,3 (1Grad. Sch. Sci., Univ. Tokyo, 2Comput. Sci., KIAS, 3Center In-Silico Protein
Sci., KIAS)
1Pos051* 金属イオンが α-ラクトアルブミンのフォールディング中間体の熱力学的安定性に与える影響
Effects of metal ions on thermodynamic stability of folding intermediates of α-lactalbumin
Reina Shinozaki, Michio Iwaoka (Dep. Chem., Sch. Sci., Tokai Univ.)
1Pos052 ウマアポミオグロビンの pH によるフォールディング機構
Mechanism of pH-induced folding of horse apomyoglobin studied by a statistical mechanical model
Takuya Mizukami, Yosuke Sakuma, Kosuke Maki (Grad. Sch. Sci., Nagoya Univ.)
1Pos053 SWAXS 解析によるトレハロースがミオグロビン構造へ与える効果の解明
SWAXS analysis on effect of trehalose on myoglobin structure
Satoshi Ajito, Mitsuhiro Hirai (Grad. Sch. Sci., Univ. Gunma)
1Pos054* Conformational Diversity in the Intrinsically Disordered HIV-1 Tat Protein induced by Zinc and pH
Tomoko Kunihara, Yuuki Hayashi, Hisashi Kudo, Hidenobu Kawai, Yoshiki Oka, Munehito Arai (Dept. Life Sci., Univ. Tokyo)
1Pos055 ＮＭＲ測定と同期したアゾベンゼン架橋剤の光異性化反応によるＧＢ１タンパク質のフォールディング操作

1Pos056

Manipulating Protein GB1 Folding Using Photoisomerization of an Azobenzene Cross-Linker Synchronously with NMR Observation
Toshio Nagashima, Keisuke Ueda, Toshio Yamazaki (RIKEN CLST)
レプリカ交換分子動力学シミュレーションによる pH に依存したポリグルタミン酸の構造変化の研究
Replica-exchange molecular dynamics study of pH dependent structural changes of polyglutamic acids
Ryosuke Iwai1, Tetsuro Nagai2, Kota Kasahara3, Takuya Takahashi3 (1Grad. Sci. Life Sci., Ritsumeikan Univ., 2Dept. of Phys., Nagoya Univ., 3Coll.
Life. Sci., Ritsumeikan Univ.)

1Pos057* Secondary structural change of glucagon during fibril formation process with DMPC lipid bilayers as revealed by 13C solid-state NMR
Kazumi Haya, Izuru Kawamura, Akira Naito (Grad. Sch. Eng., Yokohama Natl. Univ.)
1Pos058 天然変性タンパク質 c-Jun と転写コアクチベータ CBP の KIX ドメインの相互作用
Interaction of the intrinsically disordered c-Jun with the KIX domain of the transcriptional coactivator CBP
1Pos059

Satoru Yoshizaki1, Tomoko Kunihara2, Yuuki Hayashi1,2, Munehito Arai1,2 (1Dept. Integrated Sci., Univ. Tokyo, 2Dept. Life Sci., Univ. Tokyo)
ヒト α-シヌクレインのダイナミクスとアミロイド線維形成のしやすさの関係
Relationship between the dynamics of human α-synuclein and its propensity to form amyloid fibrils
Fumiaki Kono1, Tatsuhito Matsuo1, Taiki Tominaga2, Kaoru Shibata3, Katsuya Araki4, Hideki Mochizuki4, Satoru Fujiwara1 (1QuBS, QST,

2CROSS-Tokai, 3J-PARC Center, 4Osaka Univ. Grad. Sch. Med.)
1Pos060* 中間状態で阻害するフィブリノーゲンのアミロイド線維化抑制効果
Fibrinogen inhibits amyloid fibrillation by stopping at the stage of intermediates
Taiki Akai (Grad. Sch. of Sci., Kobe Univ.)

1Pos061

1Pos062

ポリグルタミン酸の構造特性に関する陽溶媒における効率的な分子動力学による研究
Structural feature of polyglutamic acids studied by enhanced molecular dynamics with explicit solvent
Tetsuro Nagai1, Ryosuke Iwai2 (1Dept. of Phys., Nagoya Univ., 2Grad. Sci. Life Sci., Ritsumeikan Univ.)
Control and biophysical characterization of soluble protein oligomers using short peptide tags
Md. Golam Kabir1, Mohammad Monirul Islam2, Tomonori Saotome1, Yutaka Kuroda1 (1Tokyo Univ. Agri. Eng. Kuroda lab Biotechnology andLife science, 2University of Chittagong, BANGLADESH)
1Pos063* 複数のアミロイド性ペプチドを含む複雑な系におけるアミロイド線維形成
Amyloid Fibrillation in Promiscuous Systems Containing Various Amyloidogenic Peptides
Hiroya Muta1, Masatomo So1, Kazumasa Sakurai2, Yuji Goto1 (1IPR, Osaka Univ., 2High Pressure Protein Res. Cent., Inst. for Advanced Tech.,
Kinki Univ.)

蛋白質：機能 / Protein: Function
1Pos064

光合成生物及び非光合成生物由来 ferredoxin-NADPH 酸化還元酵素触媒反応の可逆性
Reversibility of the redox reactions catalyzed by ferredoxin-NADPH oxidoreductases from phototroph and heterotroph
Daisuke Seo (Nat. Sci. Tec., Kanazawa Univ.)– S84 –

1Pos065

高活性型 CaMKIδ(1-299)のキナーゼ研究への活用
Application of high active form CaMKIδ(1-299) for the study of protein kinase
Yukako Senga1, Kazutoshi Akizuki2, Syouichi Katayama3, Yasushi Shigeri4, Isamu Kameshita2, Atsuhiko Ishida5, Noriyuki Sueyoshi2 (1BMRI,

1Pos066

AIST, 2Dept. Appl. Biol. Sci., Fac. Agr., Kagawa Univ., 3Dept. of Pharm., Coll. of Pharm., Ritsumeikan Univ., 4HRI, AIST, 5Grad. Sch. Integr. Arts
Sci., Hiroshima Univ.)
システイン残基修飾によるピルビン酸デヒドロゲナーゼキナーゼ 2 の動的構造変化
Dynamical structural changes of pyruvate dehydrogenase kinase 2 by modification of cysteine
Kyoka Kaiya1, Yasuhiro Fuzino2, Katumi Doi3, Etuko Nishimoto3, Yasuaki Hiromasa3 (1Grad. Sch. Bioresour. Bioenviron. Sci., Kyushu Univ.,
2Div. Arts and Science, Kyushu Univ., 3Fac. Agr., Kyushu Univ.)

1Pos067

1Pos068

1Pos069

光照射を利用した硫酸還元菌由来[NiFe]ヒドロゲナーゼの活性化機構の FT-IR 研究
FT-IR studies on the activation mechanism of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F using light irradiation
Hulin Tai1,2, Liyang Xu1, Seiya Inoue3, Koji Nishikawa3, Yoshiki Higuchi2,3, Shun Hirota1,2 (1Grad. Sch. Mat. Sci., NAIST, 2CREST, JST, 3Grad.
Sch. Life Sci., Univ. Hyogo)
アフリカツメガエル由来(6−4)光回復酵素の 4 番目の電子移動トリプトファンの解析
Analysis of the fourth electron-transferring tryptophan in Xenopus laevis (6-4) photolyase
Takahiro Kanda, Junpei Yamamoto, Shigenori Iwai (Grad. Sch. Eng of Sci., Univ., Osaka)
分子シミュレーションによるエピジェネティックな酵素に対する基質の結合選択性の研究
Study for the Ligand Binding Selectivity of Epigenetic Enzymes by using Molecular Simulations
Shuichiro Tsukamoto1,3, Yoshitake Sakae1, Yukihiro Itoh2,3, Takayoshi Suzuki2,3, Yuko Okamoto1,3,4,5,6 (1Grad. Sch. Sci., Nagoya Univ., 2Grad.
Sch. Med. Sci., Kyoto Pref. Univ. Med., 3JST-CREST, 4Struc. Bio. Res. Cen., Grad. Sch. Sci., Nagoya Univ., 5Cen. Comput. Sci., Grad. Sch. Eng.,

1Pos070

1Pos071

1Pos072

Nagoya Univ., 6Info. Tech. Cen., Nagoya Univ.)
分子動力学シミュレーションによる MutS の homoduplex DNA と mismatch DNA の認識メカニズム解析
Analysis of recognition of homoduplex and mismatched DNA by MutS by MD simulations
Hisashi Ishida, Atsushi Matsumoto (National Institutes for Quantum and Radiological Science and Technology, Molecular Modeling and
Simulation Group)
ONIOM 法を用いたアデニル酸キナーゼ反応機構に関する計算化学的研究
Computational Study on the Reaction Mechanism of Adenylate Kinase with ONIOM method
Kenshu Kamiya (Dept. of Phys., Sch. of Sci., Kitasato Univ.)
Evolutionary optimisation of elastic network structures: Models of allosteric proteins
Holger Flechsig (Hiroshima University)

蛋白質工学 / Protein: Engineering
1Pos073

バクテリオロドプシンの構造・機能特性に対する物理架橋 PVA ハイドロゲル中への固定の影響
Effects of Immobilization of Bacteriorhodopsin with Poly(Vinyl Alcohol) Hydrogels on Its Structural and Functional Properties
Hikaru Tanaka1, Yasunori Yokoyama1, Hiroshi Takahashi2, Takashi Kikukawa3, Masashi Sonoyama2, Koshi Takenaka1 (1Grad. Sch. Eng., Nagoya

1Pos074

Univ., 2Grad. Sch. Sci. & Tech., Gunma Univ., 3Grad. Sch. Life. Sci., Hokkaido Univ.)
Thermo-induced phase separation dynamics of a biopolymer model on water-in-oil droplets
Keitaro Horii1, Kazunari Yoshida2, Azusa Saito3, Akito Takashima1, Izumi Nishio1 (1Grad. Sch. of Sci. & Eng., Univ. of Aoyama, 2Grad. Sch. of

1Pos075

Med., Univ. of Tokyo, 3Grad. Sch. of Sci. & Eng., Univ. of Yamagata)
タンパク質中に生成した金ナノクラスターの発光特性
Emission property of Au Nanoclusters Formed in Protein

1Pos076

Takuma Dezawa1, Hamza Al-kindi1, Izabela Rzeznicka2, Hiroshi Fukumura1, Yutaka Shibata1 (1Grad. Sch. Sci., Univ. Tohoku, 2Grad. Sch. Eng. &Sci., ShibauraInst Tech.)
青色光によるバクテリオロドプシン色素再生に対する脂質膜相転移の影響
Effects of Lipid Phase Transition on Chromophore Regeneration of Bleached Bacteriorhodopsin in Bilayer Vesicles by Blue Light
Irradiation
Shunsuke Yano1, Kentarou Motegi1, Hikaru Tanaka1, Yasunori Yokoyama1, Masashi Sonoyama2, Koshi Takenaka1 (1Grad. Sch. Eng., Nagoya

1Pos077

Univ., 2Grad. Sch. Sci. & Tech., Gunma Univ.)
ATP 結合タンパクのゼロからのデザイン
Design of ATP-binding protein from scratch

Kengo Nakamura1,2, Takahiro Kosugi1,2, Nobuyasu Koga1,2,3 (1IMS CIMoS, 2SOKENDAI, 3JST PRESTO)
1Pos078* 合理的設計による抗体精製用リガンド FPA の開発
Rational design of FPA, a ligand for antibody purification
Yoshiki Oka1, Taihei Sawada1, Takahiro Watanabe1, Hisashi Kudo1, Manami Wada1, Hidenobu Kawai1, Mari Chang2, Yuuki Hayashi1, Munehito
1Pos079

Arai1,2 (1Dept. Life Sci., Univ. Tokyo, 2Dept. Phys., Univ. Tokyo)
新規酸化還元応答蛍光タンパク質の作成
New design of redox sensitive fluorescence proteins
Kazunori Sugiura1,2, Akiyoshi Higo1,2, Toru Hisabori1,2 (1CLS, Tokyo Tech., 2CREST, JST)– S85 –

1Pos080

1Pos081

自然界のタンパク質を大きく改造して創るヘム結合タンパク質
Computational design of heme-binding proteins by largely remodeling naturally occurring proteins
Yoshitaka Moriwaki1, Nobuyasu Koga1,2 (1CiMoS, IMS, 2JST, PRESTO)
Selection of Ru(bpy)32+ motifs from a randomized peptide libraryMarziyeh Karimiavargani1, Seiichi Tada2, Noriko Minagawa2, Takuji Hirose2, Yoshihiro Ito2, Takanori Uzawa2 (1Graduate school of Science andEngineering, Saitama University, 2Nano Medical Engineering Laboratory, RIKEN)
複数の遺伝子群の共進化を可能とする完全試験管内選択系の開発
Development of a totally in vitro selection system for co-evolution of plural genes
Asuka Ueki, Kei Fujiwara, Nobuhide Doi (Grad. Sch. Sci. Tech., Keio Univ.)
1Pos083* ファージディスプレイ法を用いたタンパク質デザインへの応用を目指した蛍光一分子ソーターの開発
Development of a single-molecular sorting system based on fluorescence detection for protein design using phage display method

1Pos082

Yuki Shimizu1,2, Naoki Mikoshiba1,3, Seiji Sakamoto1,2, Hiroyuki Oikawa1,2,3, Kiyoto Kamagata1,2,3, Takehiko Wada1,2, Satoshi Takahashi1,2,3
1Pos084(1IMRAM, Tohoku Univ., 2Grad. Sch. Sci., Tohoku Univ., 3Grad. Sch. Life Sci., Tohoku Univ.)
進化分子工学に向けたスクリーニングシステムの開発
Development of an integrated femtoliter chamber array system for directed evolution of protein molecules
Yi Zhang, Hiroto Kizoe, Yoshihiro Minagawa, Kazuhito Tabata, Hiroyuki Noji (Grad. Sch. Eng., Univ. Tokyo)

ヘム蛋白質 / Heme proteins
1Pos085

チトクロム c とチトクロム酸化酵素の複合体構造が示す新しいタンパク質間相互作用様式
Complex structure of cytochrome c and cytochrome c oxidase shows a novel inter-protein interaction mode
Satoru Shimada1, Kyoko Shinzawa-Itoh1, Junpei Baba1, Shimpei Aoe1, Atsuhiro Shimada1, Eiki Yamashita2, Jiyoung Kang1, Masaru Tateno1,

1Pos086

Shinya Yoshikawa1, Tomitake Tsukihara1,2 (1Picobiology Inst., Grad. Sch. Life Sci., Univ. Hyogo, 2Inst. Protein Res., Osaka Univ.)
蛍光偏光解消度を利用したシトクロム c-シトクロム c 酸化酵素間電子伝達複合体形成における相互作用解析
Interaction analysis of electron transfer complex formation between cytochrome c-cytochrome c oxidase using fluorescence anisotropy
Hiroshi Kagaya1, Wataru Sato1, Takeshi Uchida1,2, Kyoko Itoh－Shinzawa3, Shinya Yoshikawa3, Koichiro Ishimori1,2 (1Grad. Sch. of Chem. Sci.

and Eng., Hokkaido Univ., 2Fac. of Sci., Hokkaido Univ., 3Grad. Sch. of Life Sci., Univ. of Hyogo)
1Pos087 チトクロムｃ酸化酵素の水素結合状態変化の酸素還元反応への影響
The effect of the hydrogen bond network on the oxygen reduction of cytochrome c oxidase
Yudai Aoyagi, Tatsuhito Nishiguchi, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Satoru Nakashima, Takashi Ogura (Grad. Sch. Lif. Sci., Univ.
Hyogo)
1Pos088 時間分解共鳴ラマン分光法によるチトクローム酸化酵素の共役機構
Coupling mechanism of Cytochrome c oxidase studied by time-resolved resonance Raman spectroscopy
Satoru Nakashima, Yoshiyuki Nakagawa, Kyoko Itoh-Shinzawa, Shinya Yoshikawa, Takashi Ogura (Grad. Sch. Sci., Univ. Hyogo)
1Pos089* シトクロム c–シトクロム c 酸化酵素間の電子伝達複合体形成における脱水和の機能的意義
Functional significance of dehydration for formation of electron transfer complex between cytochrome c and cytochrome c oxidase
Wataru Sato1, Kyoko Shinzawa-Itoh3, Takeshi Uchida2, Peter Brzezinski4, Shinya Yoshikawa3, Koichiro Ishimori2 (1Grad. Sch. of Chem. Sci. and
1Pos090

1Pos091

Eng., Hokkaido Univ., 2Fac. of Sci., Hokkaido Univ., 3Grad. Sch. of Life Sci., Hyogo Univ., 4Dept. of Biochem. and Biophys., Stockholm Univ.)
呼吸鎖ヘム・銅酸素還元酵素スーパーファミリーのプロトン輸送経路の構造解析
Structural analysis of the proton transfer pathway in respiratory heme-copper oxygen reductase superfamily
Kazumasa Muramoto (Grad. Sch. of Life Sci., Univ. of Hyogo)
ナノリットルフロー時間分解可視・赤外分光法を用いた一酸化窒素還元酵素の短寿命反応過渡種の計測
Detection of Short-Lived Reaction Species of Nitric Oxide Reductase Using Nanoliter-Flow Time-Resolved Visible/IR Spectroscopy
Hanae Takeda1, Tetsunari Kimura2, Shoko Ishii1, Takehiko Tosha3, Yoshitsugu Shiro1,3, Minoru Kubo3,4 (1Grad. Sch. Sci., Univ. Hyogo, 2Grad.

1Pos092

Sch. Sci., Kobe Univ., 3SPring-8 Center, RIKEN, 4JST PRESTO)
金電極上に固定化した一酸化窒素還元酵素の電気化学的還元活性Electrochemical reduction activity of nitric oxide reductase immobilized on Au electrodes
Shogo Nakagawa1, Masaru Kato1,2, Takehiko Tosya3, Ichizo Yagi1,2 (1Grad. Sch. Environ. Sci., Hokkaido Univ., 2Faculty of Environ. Earth Sci.,

1Pos093

Hokkaido Univ., 3RIKEN)
ナノディスクに再構成した Vibrio cholerae 由来シトクロム cbb3 の構造，機能的評価
Structural and functional characterization of nanodisc-reconstituted cytochrome cbb3 oxidase from Vibrio cholerae
Masanao Inoue1, Akihiro Shibata1, Mizue Imai1, Takeshi Uchida2, Kazumasa Muramoto3, Noritsugu Shiro3, Shinya Furukawa3, Koichiro Ishimori2(1Graduate School of Science, Hokkaido University, 2Graduate School of Science, Hokkaido University, 3Graduate School of Life Science, Hyogo
University)
1Pos094* シトクロム c とカルジオリピン含有バイセルの相互作用の溶液 NMR 解析
Solution NMR characterization of the interaction between cyt c and cardiolipin-incorporated bicelles
Hisashi Kobayashi, Satoshi Nagao, Shun Hirota (Grad. Sch. Mat. Sci., Nara Inst. Sci. Tech.)– S86 –

膜蛋白質 / Membrane proteins
1Pos095* Molecular mechanism of the ATP-dependent modulation of the Mg2+ channel MgtE for Mg2+ homeositasis
Atsuhiro Tomita1, Mingfeng Zhang2, Hironori Takeda3, Fei Jin2, Tatsuro Maruyama4, Masanori Osawa4, Ryuichiro Ishitani1, Ichio Shimada4,
Zhiqiang Yan2, Motoyuki Hattori2, Osamu Nureki1 (1Grad. Sch. Sci., Univ. Tokyo, 2Sch. Life Sci., Fudan Univ., 3Fac. Life Sci., Kyoto Sangyo Univ.,
4Grad. Sch. Pharm., Univ. Tokyo)

1Pos096

チトクロム酸化酵素の高分解能結晶構造から明らかとなった高効率プロトンポンプ機構
High-resolution crystal structure of cytochrome c oxidase reveals the mechanism of highly efficient proton pumping
Atsuhiro Shimada1, Naomine Yano1, Kazumasa Muramoto1, Eiki Yamashita2,3, Kyoko Shinzawa-Itoh1, Tomitake Tsukihara1,2, Shinya Yoshikawa1

1Pos097

1Pos098

1Pos099

1Pos100(1Picobiol. Inst., Univ. Hyogo, 2Inst. Protein Res., Osaka Univ., 3SPring-8, RIKEN)
立体構造に基づいた原核生物由来ナトリウムチャネルにおける選択性フィルターの変異体解析
Structural and mutational analysis of the selectivity filter of prokaryotic sodium channel
Katsumasa Irie1,2, Yukari Haga2, Shun Nakamura2, Yoshinori Fujiyoshi1,2 (1CeSPI, Nagoya Univ., 2Grad. Sch. Pharm. Nagoya Univ.)
X 線 1 分子動態計測法への試料温度ジャンプシステムの導入
The introduction of temperature-jump system to the Diffracted X-ray Tracking (DXT)
Hirofumi Shimizu, Masayuki Iwamoto (Univ. Fukui. Fac. Med. Sci.)
原子分解能のシミュレーションによって明らかになった ADP/ATP 膜輸送体の交互アクセス機構
Deciphering Alternating Access Mechanism of a Mitochondrial ADP/ATP Membrane Transporter with Atomistic Simulations
Koichi Tamura1, Shigehiko Hayashi2 (1RIKEN AICS, 2Grad. Sch. Sci., Kyoto Univ.)
サーモフィリックロドプシンの極めて高い熱安定性に対する統計熱力学
Statistical Thermodynamics for Remarkably High Thermal Stability of Thermophilic Rhodopsin
Satoshi Yasuda1,2,3, Yuta Kajiwara4, Takeshi Murata1,2,5, Masahiro Kinoshita3 (1Grad. Sch. Sci., Chiba Univ., 2MCRC, Chiba Univ., 3IAE, Kyoto

Univ., 4Grad. Sch. Ener. Sci., Kyoto Univ., 5PRESTO)
1Pos101* 脂質二分子膜中におけるポア形成ペプチドの分子メカニズム解明に向けたモデルペプチドのチャネル電流測定
Systematically designed model pore-forming peptides study on molecular mechanism in lipid bilayers using channel current recording
Yusuke Sekiya1, Hirokazu Watanabe1, Kenji Usui2, Ryuji Kawano1 (1Tokyo Univ. Agr. Tech., 2Konan Univ.)
1Pos102* 補酵素フラビンの置換による微生物外膜シトクロムのプロトン移動の発見
Proton transfer reaction in outer-membrane flavocytochromes revealed by replacement of flavin cofactor
1Pos103
1Pos104

Yoshihide Tokunou1, Kazuhito Hashimoto2, Akihiro Okamoto2 (1Dept. of Appl. Chem., Univ. Tokyo, 2Natl. Inst. for Mater. Sci.)
Toward the elucidation of structure/function relationship of transport proteins
Naoki Soga1, Rikiya Watanabe1,2, Hiroyuki Noji1 (1Dept. of App. Chem., The University of Tokyo, 2PRESTO, JST)
生細胞における G タンパク質共役型受容体の拡散・機能連関の比較解析
Comparative analysis of diffusion-function relationship of G protein-coupled receptors on the living cell surface
Masataka Yanagawa1, Michio Hiroshima1,2, Yuichi Togashi3, Takahiro Yamashita4, Yoshinori Shichida4, Masayuki Murata5, Masahiro Ueda2,6,
Yasushi Sako1 (1Cellular Informatics Lab., RIKEN, 2QBiC, RIKEN, 3RcMcD, Hiroshima Univ., 4Dept. Biophys., Grad. Sci., Kyoto Univ., 5Dept. Life

1Pos105

1Pos106

1Pos107

1Pos108

Sci., Grad. Arts and Sci., Univ. Tokyo, 6Grad. Frontier Biosci., Osaka Univ.)
Trafficking of endocytic PAR-1 carrier vesicles in cancer cell
Seohyun Lee1, Kohsuke Gonda2, Hideo Higuchi1 (1Graduate school of science, University of Tokyo, 2Graduate school of medicine, Tohoku
university)
ミトコンドリア内膜タンパク質のマイクロ流路デバイスによる実時間解析
The real-time analysis of respiratory chain complex I on mitochondrial inner membrane by using microfluidic device
Yuji Kimura, Sayaka Kazami, Yu Hashimoto, Hiroyasu Itoh (Tsukuba Research Center, Hamamatsu Photonics KK)
細胞シグナリングに関与する上皮成長因子受容体クラスターのコレステロールを介した形成メカニズム
Cholesterol Mediated Mechanism for Signaling Cluster Formation of Epidmal Growth Factor Receptorer
Michio Hiroshima1,2, Masahiro Ueda1, Yasushi Sako2 (1RIKEN QBiC, 2RIKEN Cellular Informatics Laboratory)
ヨクトリットルスケール空間において粘性が DNA の運動に与える影響の評価
Evaluation of viscosity effect on DNA movement in yocto (10-24) liter space
Masaki Matsushita, Hirokazu Watanabe, Masayuki Ohara, Ryuji Kawano (Life Sci. Biotech., Tokyo Univ. Agri. Tech.)

核酸結合蛋白質 / Nucleic acid binding proteins
ヌクレオソームスライディングの分子機構に関する分子シミュレーション研究
Molecular Mechanisms of Nucleosome Sliding Revealed by Coarse-Grained Molecular Dynamics Simulation
Toru Niina, Shoji Takada (Graduate School of Science, Kyoto Univ.)
1Pos110* 多分子及び一分子測定により解明されたがん抑制タンパク質 p53 の超高速セグメント間移動
Ultrafast intersegmental transfer of a tumor suppressor p53 investigated by ensemble and single-molecule measurements

1Pos109

1Pos111

Yuji Itoh1,2, Agato Murata1,2, Satoshi Takahashi1,2, Kiyoto Kamagata1,2 (1IMRAM, Univ. Tohoku, 2Grad. Sch. Sci., Univ. Tohoku)
大腸菌非六量体型 DNA ヘリカーゼ UvrD 多量体の 1 分子 FRET イメージング
Single-molecule FRET imaging of the oligomeric form of the non-hexameric Escherichia coli helicase UvrD
Hiroaki Yokota (Biophotonics lab, GPI)

– S87 –

1Pos112

Identification of initial ES complex of topoisomerase IIβ and target DNA employing molecular dynamics docking simulation
Kakeru Sakabe, Hiroshi Nisihigami, Jiyoung Kan, Masaru Tateno (Grad. Sch. Sci., Univ. Hyogo)
1Pos113* Elongation of Intrinsically Disordered Linker in p53 and the Effects on DNA Binding and Sliding Ability
Dwiky Rendra Graha Subekti1,2, Agato Murata1,2, Yuji Ito1,2, Satoshi Takahashi1, Kiyoto Kamagata1 (1IMRAM, Tohoku Univ., 2Grad. Sch. Sci.,
Tohoku Univ.)

1Pos114

Nucleoprotein Filament Assembly Dynamics of Dmc1 and Rad51 Recombinases
Sheng-Yao Lin1, Wen-Hsuan Chang1, Chih-Yuan Kao2, Hung-Yuan Chi2, Hung-Wen Li1 (1Department of Chemistry, National Taiwan University,

1Pos115

1Pos116

Taipei, Taiwan, 2Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan)
リバースジャイレースによるバブル DNA 超らせん導入の物理機構
Physical mechanism of introducing positive supercoils into bubble DNA by reverse gyrase
Ryota Moritake, Takato Sato, Yuta Suzuki, Mitsunori Takano (Dept. of Pure & Appl. Phys., Waseda Univ.)
Single molecule FRET measurements of Cas9 conformation change
Kazushi Isomura1, Shohei Kajimoto2, Saki Osuka3, Hiroshi Nishimasu2, Tomohiro Shima1, Tomotaka Komori1, Osamu Nureki2, Sotaro Uemura1(1Uemura lab., Grad. Sch. Sci., Univ. Tokyo, 2Nureki lab., Grad. Sch. Sci., Univ. Tokyo, 3Dep. Bio., Sci., Univ. Tokyo)
1Pos117* Investigate how mSWI5-SFR1 complex facilitates mRAD51 presynaptic filament formation using single-molecule approaches

1Pos118

Chih-Hao Lu1, Guan-Chin Su2, Peter Chi2, Hung-Wen Li1 (1Dept. of Chemistry, Nat'l Taiwan Univ., 2Institute of Biochemical Science, Nat'l Taiwan
Univ.)
粗視化シミュレーションによる障害物存在下での DNA 結合タンパク質挙動研究
The movement of DNA binding protein including obstacles along DNA
Mami Saito1, Shoji Takada1, Tsuyoshi Terakawa2 (1Grad. Sch. Sci., Uni. Kyoto, 2Medical Center, Uni. Columbia)

核酸：構造・物性 / Nucleic acid: Structure & Property
1Pos119

1Pos120

1Pos1211Pos122
1Pos123
1Pos124

結晶構造中で観察される DNA 構造ゆらぎの網羅解析
DNA conformational transition inferred from re-evaluation of m|Fo|-D|Fc| electron density maps
Tomoko Sunami1, Toshiyuki Chatake2, Hidetoshi Kono1 (1National Institutes for Quantum and Radiological Science and Technology, 2Kyoto
University Research Reactor Institute)
ＤＮＡ高次構造の捩じれ速度依存性
Dependence of twisting velocity on higher order structure of DNA
Kotaro Yoshida, Yoshihiro Murayama (Tokyo Univ. of Agri. and Tech.)
マイクロ液滴界面を利用した RNA 転写配列を有する DNA マイクロ構造体の構築
Construction of DNA micro-structures with RNA transcription sequences using the interface of microdroplets
Risa Watanabe1, Masamune Morita1, Miho Yanagisawa2, Masahiro Takinoue1 (1Dept. Comput. Sci., Tokyo Tech., 2Dept. Appl. Phys., Tokyo Univ.
Agri. Tech.)Phase transition of genomic DNA molecules in solutions with different concentration of propanol
Yue Ma1, Yuko Yoshikawa2, Koichiro Sadakane1, Takahiro Kenmotsu1, Kenichi Yoshikawa1 (1Doshisha University, 2Ritsumeikan University)
Nucleic Acid Folding Revealed From Replica Exchange Molecular Dynamics
Jacob Swadling (University of Tokyo)
荷電脂質膜表面上での自己組織化 DNA マイクロ構造の形成
Formation of self-assembled DNA microarchitectures on a cationic lipid membrane surface
Masamune Morita1, M. Shin-ichiro Nomura2, Satoshi Murata2, Miho Yanagisawa3, Masahiro Takinoue1 (1Dept. Comput. Sci., Tokyo Tech., 2Dept.
Robotics, Tohoku University, 3Dept. Appl. Phys., Tokyo Univ. Agri. Tech.)1Pos125

1Pos126

DNA 光修復活性を有する DNA 酵素の赤外分光解析
FTIR spectroscopic analysis of a DNAzyme possessing DNA photorepair activity
Yuhi Kurahashi, Wijaya I M. Mahaputra, Tatsuya Iwata, Hideki Kandori (Nagoya Institute of Technology)
Sub-millisecond folding dynamics of preQ1 riboswitch studied by two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS)
Bidyut Sarkar1, Kunihiko Ishii1,2, Tahei Tahara1,2 (1Molecular Spectroscopy Laboratory, RIKEN, 2RIKEN Center for Advanced Photonics)

分子モーター / Molecular motor
1Pos127

1Pos128

1Pos129

F1-ATPase の回転における加水分解待ち状態から ATP 結合待ち状態への構造遷移
Conformational transition from catalytic dwell to ATP-binding dwell in F1-ATPase rotation
Kei-ichi Okazaki1,2, Mitsuhiro Sugawa3, Gerhard Hummer2 (1IMS, 2MPI Biophysics, 3Univ. of Tokyo)
a-subunit ヘリックスが傾いた新構造における FO 回転分子モーターのイオン伝導経路解析
Analysis of the ion pathway of FO molecular motor using the revised structure with tilted a-subunit helices
Kota Tezuka, Ryoichi Kiyama, Daiki Yamakoshi, Dan Parkin, Mitsunori Takano (Dept. of Pure & Appl. Phys., Waseda Univ.)
好熱菌由来の回転モーター F1 の燐酸解離のタイミング
Timing of Pi release in the rotary motor thermophilic F1 (TF1)
Kengo Adachi1, Kazuhiro Oiwa2, Masasuke Yoshida3, Taro Uyeda1, Kazuhiko Kinosita, Jr.1 (1Dept. Physics, Waseda Univ., 2Adv. ICT Res. Inst.,
NICT, 3Dept. Mol. Biosci., Kyoto Sangyo Univ.)– S88 –

1Pos130* F1-ATPase の制御因子としての ε サブユニットの ATP 解離反応における役割
Role of ε subunit on ATP dissociation as a regulator for F1-ATPase

1Pos131

Makoto Genda1, Rikiya Watanabe1, Yasuyuki Yamada2, Hiroyuki Noji1 (1Graduate School of Engineering, University of Tokyo, 2Department ofLife Science, Rikkyo University)
F1-ATPase の P-loop 変異体におけるリン酸解離の機構
The kinetics of Pi release in F1-ATPase investigated with P-loop mutations
Hiroka Narita1, Hitoshi Hoshina1, Hikaru Yoshida1, Yohei Nakayama1, Shoichi Toyabe2, Hiroshi Ueno3, Eiro Muneyuki1 (1Dept. of Phys., Chuo

Univ., 2Dept. Appl. Phys., Tohoku Univ., 3Dept. Appl. Chem., Tokyo Univ.)
1Pos132* 腸球菌 V-ATPase の Na+濃度依存 ATPase 活性を促進する化合物の同定
Identification of accelerators on Na+-depending ATPase activity of Enterococcus hirae V-ATPase
Senka Gi1, Lica Fabiana Yakushiji1, Hiroshi Ueno2, Hiroyuki Noji2, Takayoshi Arai1, Katsuhiko Moriyama1, Hideo Togo1, Takeshi Murata1,3
1Pos133(1Grad. Sch. Sci., Univ. Chiba, 2Grad. Sch. Eng., Univ. Tokyo, 3PRESTO, JST)
キネシンによる微小管の構造変化
A novel function of kinesin-1: changing microtubule conformation that accelerates successive kinesin binding
Tomohiro Shima1,2, Manatsu Morikawa3, Junichi Kaneshiro1, Taketoshi Kambara1, Shinji Kamimura4, Toshiki Yagi5, Hiroyuki Iwamoto6, Taro
Ichimura1, Tomonobu Watanabe1, Sotaro Uemura2, Ryo Nitta7, Yasushi Okada1,2, Nobutaka Hirokawa3 (1RIKEN QBiC, 2Grad. Sch. Sci., Univ.

1Pos134

Tokyo, 3Grad. Sch. Med., Univ. Tokyo, 4Dept. Biol. Sci., Chuo Univ., 5Dept. Life Sci., Pref. Univ. Hiroshima, 6SPring-8, JASRI, 7RIKEN CLST)
二量体分子モーターの歩行に関する統一モデル
A unified walking model for dimeric motor proteins

1Pos135

Kazuo Sasaki1, Motoshi Kaya2, Hideo Higuchi2 (1Grad. Sch. Eng., Tohoku Univ., 2Grad. Sch. Sci., Univ. Tokyo)
タンデムに２つの頭部をつないだキネシンを用いた選択的な前方へのステップの研究
Preferential forward stepping mechanism of kinesin-1 studied using tandemly joined two-headed monomer

Kohei Matsuzaki1, Hiroshi Isojima1, Sawako Enoki2, Hiroyuki Noji2, Michio Tomishige1 (1Dept. Appl. Phys., Grad. Sch. Eng., Univ. Tokyo, 2Dept.
Appl. Chem., Grad. Sch. Eng., Univ. Tokyo)
1Pos136 光応答性蛋白質 Dronpa を利用したキネシン運動活性の光可逆的阻害
Photo-reversible inhibition of kinesin motor activity utilizing photochromic protein Dronpa
Kohei Uchida, Shinsaku Maruta, Kazunori Kondo (Grad. Sch. Bioinfo., Univ. Soka)
1Pos137* ゆらぎの定理を用いた非侵襲な力測定によるメラニン色素顆粒輸送のメカニズムの解明
Application of the fluctuation theorem for the non-invasive measurement of force to pigment transport in melanophores
Shin Hasegawa1, Kazuho Ikeda2, Takashi Sagawa3, Yasushi Okada2,4, Kumiko Hayashi1,5 (1Sch. Eng., Tohoku Univ., 2QBiC, RIKEN, 3NICT, 4Sch.
1Pos138

Sci., Univ. Tokyo, 5AMED, PRIME)
DNA オリガミを用いた速度の異なるキネシンによる協調運動の観察
Cooperative transport by two kinesin motors with different velocities studied using programmable DNA origami
Ryosuke Masuda1, Tsuyoshi Omi1, Yamato Niitani1, Mitsuhiro Iwaki2, Michio Tomishige1 (1Department of Applied Physics, Univ. of Tokyo,
2QBiC, RIKEN)

1Pos139
1Pos140

Inhibitory mechanism for photochromic kinesin Eg5 inhibitor composed of spiropyran derivative
Ryoma Yamamoto, Kei Sadakane, Shinsaku Maruta (Grad. Sch. Bioinfo., Univ. Soka)
1 分子 FRET 観察による細胞質ダイニンの構造変化の計測
Single molecule FRET observation of cytoplasmic dynein’s conformational change
Mikiya Sakata1, Takuya Kobayashi1, Mitsuhiro Sugawa1, Tomohiro Shima2, Junichiro Yajima1, Yoko Y. Toyoshima1 (1Grad. Sch. Arts and Sci.,

1Pos141

Univ. of Tokyo, 2Grad. Sch. Sci., Univ. of Tokyo)
微小管系モーターダイニンを基に新規アクチン系モーターをエンジニアリングする

Engineering Novel Actin-Based Molecular Motors from the Microtubule-based Motor Dynein
Akane Furuta, Kazuhiro Oiwa, Hiroaki Kojima, Ken’ya Furuta (Frontier Research Lab, NICT)
1Pos142* 細胞質ダイニンのマルチスケールシミュレーション:全原子から連続体へ
Multiscale Simulations of Cytoplasmic Dynein: From All-atom to Continuum Mechanics
Shinji Iida1,3, Benjamin Hanson4, Narutoshi Kamiya2, Genji Kurisu1, Takahide Kon3, Haruki Nakamura1, Sarah Harris4 (1IPR, Osaka Univ., 2Grad.
Sch. SS, Univ. Hyogo, 3Grad. Sch. Sci., Osaka Univ., 4Sch. Phys. Astro., Univ. Leeds)
Diffusive Component in Directed Movements of Cytoplasmic Dynein
Takayuki Torisawa, Ken’ya Furuta, Kazuhiro Oiwa, Hiroaki Kojima (NICT, Advanced ICT Research Institute)
1Pos144 細胞質ダイニンの構造変化を伴う運動メカニズムに関する分子シミュレーション研究
Molecular simulation study on the working mechanism with structural changes of cytoplasmic dynein
Shintaroh Kubo, Shoji Takada (Grad. Sci., Univ. Kyoto)1Pos145* ヒト細胞質ダイニン１分子のパワーストローク運動距離の測定
Measurement of the power stroke distance of cytoplasmic dynein motor

1Pos143

1Pos146

Yoshimi Kinoshita1, Taketoshi Kambara1,2, Kaori Nishikawa1, Motoshi Kaya1, Hideo Higuchi1 (1The University of Tokyo, 2QBiC, Riken)
ミオシン S1 によって誘起されるアクチンフィラメントの協同的構造変化の高速 AFM による観察と、その生理的意義
High-speed AFM demonstration of cooperative structural changes in actin filaments induced by myosin S1 and physiological implication
Kien Xuan Ngo1,2, Noriyuki Kodera3, Toshio Ando3, Taro Ueda1,2 (1Biomed. Res. Inst., AIST, 2Dept. Phys., Waseda Univ., 3Dept. Phys., & BioAFM FRC, Kanazawa Univ.)– S89 –

1Pos147

単一フィラメントにおけるアクチン重合・脱重合ダイナミクスの力学的制御
Mechanical manipulation of polymerization dynamics of individual actin filaments
Hiroaki Kubota1, Makito Miyazaki1,2, Taisaku Ogawa3, Togo Shimozawa4, Kazuhiko Kinosita Jr.1, Shin’ichi Ishiwata1 (1Dept. Physics, Waseda

1Pos148

1Pos149

1Pos150
1Pos151

Univ., 2Waseda Bioscience Research Institute in Singapore, Waseda Univ., 3QBiC, Riken, 4Dept. Life Sci. Med. Biosci., Waseda Univ.)
Actomyosin contraction with a contractile ring related cross-linker in an in vitro active gel model system
Kyohei Matsuda, Takuya Kobayashi, Mitsuhiro Sugawa, Yoko Y. Toyoshima, Junichiro Yajima (Department of Life Sciences, Graduate School ofArts & Sciences, The University of Tokyo)
混み合い環境でのアクチン線維の集団運動による秩序構造の出現
Appearance of ordered structure by collective motion of actin filaments in crowded environments
Takahiro Iwase, Yasuhiko Sasaki, Kuniyuki Hatori (Dept. Bio-Systems Eng., Yamagata Univ.)
Self-organizations of actin filament networks in confined spaces: A simulation study
Takahiro Nitta (Gifu Univ.)
蛍光顕微鏡および高速 AFM によるミオシンと F-アクチン間の協同的結合の経時的観察
Real-time observation of cooperative binding between myosin and F-actin by fluorescence microscopy and high-speed atomic force
microscopy
Rika Hirakawa1, Hiroaki Ueno1, Noriyuki Kodera2, Taro Q.P. Uyeda3, Kiyotaka Tokuraku1 (1Muroran Inst. Tech., 2Bio-AFM FRC, Inst. Sci.

&Eng., Kanazawa Univ., 3Waseda Univ.)
1Pos152* 枯渇力が誘起する微小管集団運動に関する研究
Study of the collective motion of microtubules induced by depletion force
Ai Saito1, Ryuhei Suzuki1, Tamanna Ishrat Fahana1, Arif Md. Rashedul Kabir2, Kazuki Sada1,2, Akira Kakugo1,2 (1Grad. Sch. of Chem. Sci. andEng., Hokkaido Univ., 2Fac. of Sci., Hokkaido Univ.)

細胞生物学 / Cell biology
1Pos153

神経細胞における細胞骨格アクチンの修復の分子メカニズム
Molecular mechanism of cytoskeletal Actin repairing in nerve cells
Tomboy Higo1, Ayumi Ishihara2, Shinji Aramaki1, Yoshiko Itou2, Takuo Yasunaga1 (1Kyushu Institute of Technology, 2Leica Microsystems)

1Pos154

超解像光学顕微鏡で観察した収縮環の計測と解析
Measurement and analysis of contractile ring observed with STED and Sim
Kaoru Katoh1,2, Keijyu Kamijo3, Minami Tanaka2, Masayuki Takahashi4, Issei Mabuchi5, Hiroshi Hosoya6 (1BioMed. Res. Inst., AIST, 2Gad. Sch.
of Life & Environ. Sci., Univ. Tsukuba, 3Sch. of Med., Tohoku Med. Pharm. Univ., 4Dept. of Chem, Fac. of Sci., Hokkaido Univ., 5Dept. of Life Sci.

1Pos155

Gakushuin Univ., 6Dept. of Biol., Fac. of Sci., Kanagawa Univ.)
アクチンフィラメントの協同的構造変化を阻害するアクチン変異の遺伝子内サプレッサー解析
Intragenic suppressor analysis of actin mutation that impairs cooperative conformational change of actin filament
Tenji Yumoto1,4, Takehiko Yoko-o2, Keiko Hirose3, Taro Uyeda4 (1Grad. Sch. Life & Env. Sci., Univ. of Tsukuba, 2Bioprod. Res. Inst., AIST,
3Biomed. Res. Inst., AIST, 4Dept. Phys., Waseda Univ.)

1Pos156

クライオ電子線トモグラフィ法で明らかにした，フィロポディア内におけるファシンによるアクチンフィラメント束化メカニズム
F-actin bundling mechanisms by fascin in filopodia was revealed by cryo-ET
Shinji Aramaki1, Kouta Mayanagi2, Kazuhiro Aoyama3,4, Takuo Yasunaga1 (1Department of Bioscience and Bioinformatics, 2Medical Institute of

1Pos157

Bioregulation, Kyushu University, 3FEI Japan, 4Research Centre for Ultra-High Voltage Electron Microscopy, Osaka University)
MEA システムを用いたニワトリ胚由来心臓組織片の薬剤応答
Drug response of embryonic chick heart tissue pieces using multi electrode array system

Yosuke Kamei1, Toshiyuki Mitsui2, Tomoyuki Kaneko1 (1LaRC,Grad. Sci. Eng., Hosei Univ., 2Dept. Math. Phys., Col. Sci. Eng., Aoyama Univ.)
1Pos158 心筋細胞の集合体に対する機械的刺激の影響
Influence of mechanical stimulus on embryonic chick heart cell aggregates
Shin Arai, Ayaha Tsuyuki, Takahiro Uehara, Kentaro Ishida, Toshiyuki Mitsui (Coll. of Sci. & Eng., Aoyama Gakuin Univ.)
1Pos159 フィードバックマイクロレオロジーによる細胞骨格の非線形力学挙動計測
Nonlinear mechanical properties of Cytoskeletons measured with Dual-Feedback Microrheology
Natsuki Honda, Kenji Nishizawa, Takayuki Ariga, Daisuke Mizuno (Kyushu University, Department of Physics)
1Pos160 ケラトサイトの運動方向を決定する２つのメカノセンシング機構
Keratocytes have hybrid mechanosensing system to decide their migration direction
Chika Okimura, Yoshiaki Iwadate (Fac. Sci., Yamaguchi Univ.)
1Pos161* 非熱的な力に駆動された細胞内部の混み合い状態
Intracellular crowding mechanics driven by athermal force
Kenji Nishizawa, Daisuke Mizuno (Grad. Sch. Sci., Kyushu Univ.)
1Pos162 心筋細胞の集合体群に与えるマルチプローブ機械的刺激の影響
Effect of Multi-probe stimuli on cardiac cell aggregates with spontaneous beatAyaha Tsuyuki, Shin Arai, Takahiro Uehara, Kentaro Ishida, Toshiyuki Mitsui (Coll. of Sci. & Eng., Aoyama Gakuin Univ.)
1Pos163 魚類表皮細胞ケラトサイトのかたち・サイズと牽引力
Relationship between traction forces, and shape and size of keratocytes
Ayane Sonoda, Chika Okimura, Yoshiaki Iwadate (Fac. Sci., Yamaguchi Univ.)– S90 –

1Pos164

アメーバ運動する線虫精子の牽引力測定
Measurement of Traction Force generated by Amoeboid Sperm of C. elegans
Midori Yoshimura1, Hikaru Emoto1, Chika Okimura2, Yoshiaki Iwadate2, Katsuya Shimabukuro1 (1Dep. of Chem. and Bio. Eng., NIT, Ube

College, 2Faculty of Sci., Yamaguchi University)
1Pos165 細胞周期進行に伴う細胞内部環境のダイナミクス
The dynamics of Intracellular Environments during Cell-cycle progression
Katsuhiro Umeda, Kenji Nishizawa, Daisuke Mizuno (Grad. Sci., Univ. Kyushu)
1Pos166* Actin Cytoskeleton Remodeling Dynamics of Adherent Cells Under Mechanical Strain of Gelatin Substrate
Kwokhoi Ng1, Kentaro Iketaki1, Ryuzo Kawamura1, Seiichiro Nakabayashi1, Yosuke Yoneyama3, Fumihiko Hakuno3, Shin-Ichiro Takahashi3,
Fumiki Yanagawa2, Toshiyuki Takagi2, Shinji Sugiura2, Toshiyuki Kanamori2, Hiroshi Yoshikawa1 (1Dept. Chem.,Saitama Univ., 2BRD.,AIST.,3GASLS., The Univ. of Tokyo)

1Pos167

1Pos168

1Pos169

血管壁内力学環境を考慮したコラーゲン微細溝基質による血管平滑筋細胞の分化制御
Control of vascular smooth muscle cell differentiation using a novel micro-grooved collagen substrate
Kazuaki Nagayama, Keiichi Uchida, Saki Takeuchi (Micro-Nano Biomechanics Laboratory, Department of Intelligent Systems Engineering,
Ibaraki University)
力学的強度の制御を可能とする光架橋性コラーゲンゲルの開発
Development of photo-cross-linked collagen gels with tunable mechanical property
Takahiro Fujisawa1, Satoru Kidoaki2 (1Grad. Sch. Eng., Kyushu Univ., 2IMCE, Kyushu Univ.)
組織切片の伸展性応答: ひび割れパターンと病態
Response of Tissue Slice to Mechanical Stretching: Characteristic Cracking Pattern Reflecting Disease State
Keisuke Danno1, Takuto Nakamura1, Naohiko Nakamura2, Kota Iguchi2, Masaya Ikegawa3, Kenichi Yoshikawa3 (1Doshisha Univ., 2Kyoto Univ.,
3Doshisha Univ.)

1Pos170

タリンとビンキュリンによる力と硬さの感知
Force- and rigidity-sensing by talin and vinculin
Hiroaki Hirata1,2,3, Keng-Hwee Chiam3, Hitoshi Tatsumi4, Chwee Teck Lim3, Masahiro Sokabe1,3 (1Nagoya Univ. Grad. Sch. Med.,

1Pos171

Mechanobiology Lab, 2R-Pharm Japan, 3Mechanobiology Inst., Natl. Univ. Singapore, 4Kanazawa Inst. Tech.)
マイクロメートルスケールの足場構造に依存した細胞性粘菌の細胞遊走Migration of Dictyostelium cells on micro-scale ridge structures
Gen Honda1, Akihiko Nakajima2, Satoshi Sawai1,2,3 (1Graduate School of Arts and Sciences, University of Tokyo, 2Research Center for Complex

Systems Biology, 3PRESTO, JST)
1Pos172* 膜タンパク質による細胞間相互作用の定量的解析
The quantitative analysis of the intercellular interaction by membrane proteins

1Pos173

1Pos174

Takumi Miyatake1,2, Yoshihisa Kaizuka2 (1Graduate School of Pure and Applied Sciences, University of Tsukuba, 2National Institute for Materials
Science)
重力下での形態形成・維持に対するアクトミオ シンネットワークの寄与
Teoretical study of contribution of YAP-dependent actomysin network to morphogenesis under gravity
Kazunori Takamiya1, Hiraku Nishimori1,2, Akinori Awazu1,2 (1Grad. Sch. Sci., Univ. Hiroshima, 2RcMcD)
Divergence of structural strategies for E-cadherin homophilic binding among bilaterians

Shigetaka Nishiguchi1,2,3, Akira Yagi3, Nobuaki Sakai3, Hiroki Oda1,2 (1JT BRH, 2Osaka Univ., 3Olympus Co.)
1Pos175* DNA hybridization を介した細胞-細胞間接着ダイナミクスの解明
Dynamics of cell-cell adhesion via DNA hybridization
Ken Sato1, Yuji Teramura2, Ryuzo Kawamura1, Naritaka Kobayashi1, Seiichiro Nakabayashi1, Hiroshi Yoshikawa1 (1Dept. Chem., Saitama Univ.,
2Dept. Bioeng., Tokyo Univ.)

1Pos176

膵島 α 細胞の分泌顆粒動態に及ぼす接着分子 CADM1 の影響
Effect of cell adhesion molecule 1 expression on intracellular granule movement in pancreatic α cells
Tadahide Furuno1, Satoru Yokawa1,2, Takanari Ikeda1, Yoshikazu Inoh1, Ryo Suzuki2, Takahiro Suzuki3, Naohide Hirashima2 (1Sch. Pharm., Aichi

1Pos177

Gakuin Univ., 2Grad. Sch. Pharm. Sci., Nagoya City Univ., 3Sch. Dent., Aichi Gakuin Univ.)
分散培養 iPS 細胞の増殖応答性に対するハイドロゲル表面へのラミニン修飾状態の本質的効果
Essential role of mannter of laminin-modification for hydrogel surface on the prolifelation activity of dissociated iPS cells

Kenta Mizumoto1, Satoru Kidoaki2 (1Grad. Sch. Eng., Univ. Kyushu, 2IMCE., Univ. Kyushu)
1Pos178 細胞内ナノ粒子導入・細胞間相互作用制御のための材料工学
Materials engineering approaches to modulate cell membrane structures for nanoparticles delivery and regulation of cell-cell interactions
Yoshihisa Kaizuka, Tomoto Ura, Hidenobu Nakao (NIMS)
1Pos179* 脂質膜に覆われた細胞サイズ生体高分子ゲルの弾性率
Elasticity of biopolymer gel in cell-sized droplet covered with a lipid membrane
Atsushi Sakai, Yoshihiro Murayama, Miho Yanagisawa (Tokyo university of Agriculture and Technology)
1Pos180 原始真核生物の細胞内小器官の 3D 構造モデルから得られる生物の新たな情報
New obvious information obtained from cell organelle 3D-structural models of primitive eukaryote
Atsuko H. Iwane1,2, Rina Nagai1,2, Hikari Mori1, Takako Ichinose1,2 (1Cell Field Struc., QBiC, Riken, 2Grad. Sch. Fronti., Biosci., Osaka Univ.)– S91 –

1Pos181
1Pos182

1Pos183

Accumulation of cargo proteins can physically trigger vesiculation in membrane trafficking system
Masashi Tachikawa (RIKEN)
多突起型チューブリン封入リポソームの形態形成メカニズム
Morphogenetic mechanism of tubulin-encapsulating giant liposomes with a hundred of membrane spines
Masahito Hayashi, Kingo Takiguchi (Grad. Sch. Sci., Nagoya Univ.)
アクトミオシンネットワークの収縮による細胞サイズ液滴の運動
Directed motion of cell-sized droplets driven by actomyosin network contraction
Yuto Sano1, Makito Miyazaki1,2, Kozue Hamao3, Shin’ichi Ishiwata1 (1Dept. Physics, Waseda Univ., 2Waseda Bioscience Research Institute inSingapore, Waseda Univ., 3Dept. Bio. Sci., Hiroshima Univ.)
1Pos184 高濃度アクチン繊維が引き起こす立体的集団運動
The collective motion and band pattern formations of sliding actin filaments driven by HMM
Yuuji Setoguchi, Hirotaka Taomori, Masayuki Hoshida, Yu Ichinose, Hajime Honda (Dept. Bioeng., Nagaoka Univ. Tech.)1Pos185 細胞に優しい三次元組織体の構築：レーザートラップと高分子の混雑効果の活用
Constructing stable cellular assembly in the absence of artificial scaffold by use of laser tweezers
Shoto Tsuji, Aoi Yoshida, Taeko Ohta, Hiroaki Taniguchi, Kenichi Yoshikawa (Doshisha University)
1Pos186 ヒト疾患診断マーカーとして有用なエキソソームの生物物理解析に適した新規分離調製法の開発
A novel isolation and preparation method for the biophysical analyses of useful exosomes as diagnostic markers for human diseases
Noriyuki Ishii, Mitsushi J. Ikemoto, Takayuki Odahara (Biomedical Research Institute, National Institute of Advanced Industrial Science andTechnology (AIST))
1Pos188* アクチンの示すミクロ顆粒系での局在性転移：細胞の混雑環境モデリング
Selective Localization of Actin in Micro-Domains under Molecular Crowding: Difference among Monomeric, Linear-Polymeric andBundling State
Naoki Nakatani1, Chen-Yang Shew2, Kanta Tsumoto3, Kingo Takiguchi4, Masahito Hayashi4, Shunsuke Tanaka4, Kenichi Yoshikawa1 (1Grad.
Sch. Life and Medical Sciences, Doshisha Univ., 2Division of Science & Technology, College of Staten Island, New York City Univ., 3Grad. Sch.
Engineering, Mie Univ., 4Grad. Sch. Science, Nagoya Univ.)

生体膜・人工膜：ダイナミクス / Biological & Artificial membrane: Dynamics
1Pos189

ジャイアントベシクルにおけるアミロイド繊維の形成
Amyloid fibril formation in giant vesicle

Tong Zhu1, Kensuke Kurihara1,2,3 (1Okazaki Institute for Integrative Bioscience, 2Institute for Molecular Science, 3Research Center for Complex
Systems Biology, The Univ. of Tokyo)
1Pos190* 人工細胞モデルを用いた生体膜融合機構の解明
Biophysical principle of membrane fusion revealed by artificial lipid vesicles
1Pos191

Yui Suzuki1, Ken Nagai1, Anatoly Zinchenko2, Tsutomu Hamada1 (1JAIST, 2Grad. Sch. of Environmental Studies, Nagoya Univ.)in vitro １分子イメージング解析により明らかになった PI(4,5)P2 依存的な PTEN の膜結合の促進・安定化
Phosphatidylinositol lipid PI(4,5)P2 enhances membrane binding of PTEN revealed by in vitro single-molecule imaging analysis
Daisuke Yoshioka1, Seiya Fukushima1,3, Daichi Okuno3, Satomi Matsuoka3, Toru Ide4, Masahiro Ueda2,3 (1Dep. Biol. Sci., Grad. Sch. of Sci.,

1Pos192
1Pos193

1Pos194

Osaka Univ., 2Grad. Sch. of Front. Biosci., Osaka Univ., 3RIKEN QBiC, 4Grad. Sch. of Nat. Sci. and Tech., Okayama Univ.)
Measurements of mitochondrial motility in cell bodyHyunjin Choi, Yuki Sugimoto, Yoshihiro Ohta (Div. of Biotech. And Life Sci., Inst. of Eng., Tokyo Univ. of Agr. and Tech.)
人工脂質膜小胞内における PIP3/PTEN traveling wave の再構成
Reconstitution of traveling wave of PIP3/PTEN on membrane in GUVs
Hitomi Matsubara (Lab. Single Molecule Biology, Grad. FBS., Osaka Univ.)
細胞透過ペプチド・オリゴアルギニンの単一ベシクルへの侵入に対する脂質組成の効果
Effects of lipid compositions on the entry of cell-penetrating peptide oligoarginine into single vesicles
Sabrina Sharmin1, Md Zahidul Islam1, Mohammad Abu Sayem Karal1, Sayed Ul Alam Shibly1, Hideo Dohra2, Masahito Yamazaki1,3,4 (1Grad.
Sch. Sci. Tech., Shizuoka Univ., 2Res. Inst. Green Sci. Tech., Shizuoka University, 3Res. Inst. Ele., Shizuoka Univ., 4Grad. Sch. Sci., Shizuoka Univ.)

1Pos195

Functional significance of trimerization in Cl- pumping properties of halorhodopsin examined by nanodisc reconstitution
Eri Hashimoto1, Kenshiro Suzuki1, Ayumi Yamamoto1, Takashi Tsukamoto2, Takeshi Uchida1,3, Takashi Kikukawa2, Makoto Demura2, Koichiro

1Pos196Ishimori1,3 (1Grad. Sch. of Chem. Sci. and Eng. Hokkaido Univ., 2Grad. Sch. of Life Sci. Hokkaido Univ., 3Fac. of Sci. Hokkaido Univ.)
脂質膜の伸展の分光学的研究
Spectroscopic Investigation of Osmotic Pressure-Induced Membrane Stretching
Chiranjib Ghatak1, Sayed Ul Alam Shibly2, Masahito Yamazaki1,2,3 (1Res. Inst. Ele., Shizuoka Univ., 2Grad. Sch. Sci. Tech., Shizuoka Univ.,
3Grad. Sch. Sci., Shizuoka Univ.)

1Pos197

細菌-膜小胞間相互作用の動態と情報伝達機構の解析
Dynamics and signal transduction of the interaction between bacteria and membrane vesicles
Yosuke Tashiro, Yusuke Hasegawa, Kotaro Takaki, Hiroyuki Futamata (Dept. of Eng., Shizuoka Univ.)– S92 –

1Pos198

マガイニン２が誘起する脂質膜中のポア形成に対する抗菌ペプチド・PGLa の効果
Effect of Antimicrobial Peptide PGLa on Magainin 2-Induced Pore Formation in Lipid Membranes
Farliza Parvez1, Md Jahangir Alam2, Hideo Dohra3, Masahito Yamazaki1,2,4 (1Grad. Sch. Sci. Tech., Shizuoka Univ., 2Res. Inst. Ele., Shizuoka
Univ., 3Res. Inst. Green Sci. Tech., Shizuoka Univ., 4Grad. Sch. Sci., Shizuoka Univ.)

生体膜･人工膜：輸送 / Biological & Artificial membrane: Transport
1Pos199

大腸菌異物排出系トランスポーター MdtB, MdtC 会合の可視化
Assembly of the xenobiotic efflux transporters MdtB and MdtC of Escherichia coli
Megumi Yamazaki1, Kentaro Yamamoto1, Masatoshi Nishikawa1, Yoshiyuki Sowa1,2, Ikuro Kawagishi1,2 (1Dept. Frontier Biosci., Hosei Univ.,
2Res. Cen. Micro-Nano Tech., Hosei Univ.)

1Pos200

1Pos201

外部環境を感知する磁場駆動型リポソームの創製
Magnetically-driven moving liposomes that sense environmental information
Mika Ebihara1, Taro Toyota2, Naoto Nemoto1 (1Grad. Sch. Sci. Eng., Saitama Univ., 2Grad. Sch. Arts Sci., Univ. of Tokyo)
ミトコンドリア密集による ATP 産生の効率化
Effective ATP generation by closely located mitochondria
Yusho Kuraoka1, Daiki Yoshimatu1, Takuya Takahashi2, Yoshihiro Ohta1 (1Div. of Biotech. And Life Sci., Inst. of Eng., Tokyo Univ. of Agr. andTech., 2Dept. of Biosci. and Bioinformatics, Ritsumeikan Univ.)

生体膜・人工膜：構造・物性 / Biological & Artificial membrane: Structure & Property
1Pos202

1Pos203

高いトポロジー種数を持つベシクルの形態：核膜形状の形成
Morphology of high-genus vesicles under pore-size constraint: Construction of nuclear envelope shape
Hiroshi Noguchi (ISSP, Univ. Tokyo)
両親媒性 DNA オリガミによる W/O マイクロエマルションの光応答性観察
Photo-responsive water-in-oil microemulsion made of amphiphilic DNA origami
Misato Tsuchiya1, Daisuke Ishikawa1, Yuki Suzuki2, Masayuki Endo3, Masahiro Takinoue1 (1Dept. Comput. Sci., Tokyo Tech., 2Fronti. Res. Inst.

1Pos204

1Pos205

1Pos206

1Pos207

1Pos208

1Pos209

1Pos210

Interdiscip. Sci., Tohoku Univ., 3WPI-iCeMS., Kyoto Univ.)
合成高分子による膜曲率の認識
Recognition of membrane curvature by synthetic amphiphilic polymers
Naho Sunagawa1, Manami Tsukamoto1, Kenichi Kuroda2, Jun-ichi Kikuchi1, Kazuma Yasuhara1 (1Grad. Sch. Mat. Sci., Nara Inst. Sci. Tech., 2Sch.
Dentistry Univ. Michigan)
膜貫通タンパク質の細胞膜上二次元拡散における二段階緩和の理論解析
Theoretical analysis of a two-step relaxation on protein diffusion in the plasma membranes
Tomonari Sumi1, Atsushi Okumoto2, Hitoshi Goto2, Hideo Sekino2 (1Res. Inst. Interdisciplinary Sci., Okayama Univ., 2Toyohashi Univ. Tech.)
肺サーファクタントタンパク質 B の N 末端による脂質単分子膜の構造変化
Morphology changes in lipid monolayers induced by the N-terminal segments of surfactant protein B
Hideyuki Nagatsuka, Masahiro Hibino (Div. Sust. Enviro. Eng., Muroran Inst. Tech.)
シトクロム P450 基質薬剤クロルゾキサゾンとホスファチジルエタノールアミン・モデル膜との相互作用
Interaction between cytochrome P450 substrate drug chlorzoxazone and phosphatidylethanolamine model membranes
Hiroshi Takahashi (Grad. Sch. Sci. Tech., Gunma Univ.)
脂質キュービック相間の相転移における方位関係
Orientational Relationships In Transformations Between Three Inverse Bicontinuous Cubic Phases of a Lipid
Toshihiko Oka1,2 (1Faculty of Science, Shizuoka University, 2Research Institute of Electronics, Shizuoka University)
ジミリストイルホスファチジルコリン–コレステロール二成分二分子膜中における構成脂質の部分モル体積
Partial molar volumes of constituent lipids in the binary bilayer of dimyristoylphosphatidylcholine and cholesterol
Nobutake Tamai1, Naohiro Takeshita2, Masaki Goto1, Hitoshi Matsuki1 (1Grad. Sch. Biosci. Bioind., Tokushima Univ., 2Grad. Sch. Adv. Tech. Sci.,
Tokushima Univ.)
部分フッ素化リン脂質と DMPC の二成分系混合膜物性に関する系統的研究
Mixing behaviors in the binary membrane of DMPC and its partially fluorinated analogues with different perfluoroalkyl chain lengths
Miki Horikoshi1, Kohei Morita1, Toshinori Motegi1, Hiroshi Takahashi1, Hideki Amii1, Toshiyuki Takagi2, Toshiyuki Kanamori2, Masashi

1Pos211

1Pos212

Sonoyama1 (1Div. Mol. Sci., Gunma Univ., 2AIST)
多電極アレイ上でのエレクトロフォーメーション法によるリポソームの作製
Preparation of liposomes by electro formation method on multi electrode array
Hayato Akizuki, Tomoyuki Kaneko (LaRC, Grad. Sci. Eng., Hosei Univ.)
PEG 脂質を導入した支持脂質二重膜の拡散特性
Effect of PEG-lipid on diffusion properties of supported lipid bilayer
Moeko Saruta, Takuhiro Otosu, Shoichi Yamaguchi (Grad. Sch. Sci. Eng., Saitama Univ.)– S93 –

1Pos213

プログラマブルな性質を有する DNA ナノプレートからなるマイクロカプセルの形成
Microcapsular compartments composed of programmable DNA nanoplates
Daisuke Ishikawa1, Yuki Suzuki2, Chikako Kurokawa3, Masayuki Ohara4, Masamune Morita1, Miho Yanagisawa3, Ryuji Kawano4, Masayuki
Endo5, Masahiro Takinoue1 (1Sch. Comput., Tokyo Tech., 2FRIS, Tohoku Univ., 3Dept. Appl. Phys., Tokyo Univ. of Agri. and Tech., 4Dept. Life Sci.
and Biotech., Tokyo Univ. of Agri. and Tech., 5WPI-iCeMS, Kyoto Univ.)

生体膜･人工膜：興奮・チャネル / Biological & Artificial membrane: Excitation & Channels
1Pos215

コリネ細菌の機械受容チャネルによる細胞力覚とグルタミン酸放出機構
Bacterial mechanosensation and glutamate export by mechanosensitive channels in Corynebacterium glutamicum
Yoshitaka Nakayama1, Kosuke Komazawa2, Navid Bavi1,3, Ken-ichi Hashimoto2, Hisashi Kawasaki2, Boris Martinac1,3 (1Victor Chang Cardiac

1Pos216

Research Institute, 2Tokyo Denki University, 3University of New South Wales)
全反射赤外分光で見る電位依存性プロトンチャネル VSOP への金属結合
Metal binding to the voltage-gated proton channel VSOP studied by ATR-FTIR
Masayo Iwaki1, Kohei Takeshita2,3,4, Yasushi Okamura5, Atushi Nakagawa2, Hideki Kandori1 (1Nagoya Inst. Tech., 2Inst. Protein Res., Osaka

1Pos217

Univ., 3Inst. Acad. Initiat., Osaka Univ., 4JST-PRESTO, 5Grad. Sch. Med., Osaka Univ.)
ナノキャビティでの K+の占有が Kv1.2 チャネルを通る K+の滑走を引き起こす
Occupancy of a K+ in the nanocavity induces K+ ions' run through the Kv1.2 channel
Takashi Sumikama, Shigetoshi Oiki (Univ. of Fukui)

1Pos218

1Pos219
1Pos223
1Pos224

1Pos225

1Pos226

KcsA チャネルの細胞内領域の荷電状態がチャネル開閉に与える影響
Effects of the electrostatic state of the cytoplasmic domain in the KcsA channel on its gating
Minako Hirano1, Toru Ide2 (1GPI, 2Okayama Univ.)
Discrimination between mitochondrial rounding and permeability transition
Takahiro Shibata, Yoshihiro Ohta (Grad. Sch. Life Sci. & Bio Tech., TUAT)Artificial bilayers formed on a solid substrate for ion-channel recordings
Toru Ide1,2, Saki Nomura1, Minako Hirano2, Junnya Ichinose1, Hiroaki Yokota2 (1Grad. Schl Sci. Tech., Okayama Univ., 2GPI)
電位依存性ホスファターゼ VSP の酵素ドメインにおける膜相互作用部位の役割
The role of membrane interacting region of phosphatase domain in voltage-sensing phosphatase (VSP)
Akira Kawanabe1, Masaki Hashimoto1, Tomoko Yonezawa1, Yuka Jinno2, Souhei Sakata2, Yasushi Okamura1 (1Osaka Univ., 2Osaka Med. Col.)
イオンチャネル機能に対する膜脂質効果の解析に向けた脂質二重膜組成の迅速変更法
Rapid replacement of the lipid bilayer composition for the analysis of the lipid-effect on the ion channel function
Masayuki Iwamoto, Shigetoshi Oiki (Dept. Mol. Physiol. & Biophys., Univ. Fukui Facult. Med. Sci.)
高速原子間力顕微鏡による K+チャネル KcsA とポア結合性サソリ毒アジトキシン-2 の結合ダイナミクスの一分子解析
Single-molecule blocking dynamics of a scorpion toxin on the KcsA potassium channel revealed by HS-AFM
Ayumi Sumino1,2, Takayuki Uchihashi3, Takashi Sumikama2, Shigetoshi Oiki2 (1JST/PRESTO, 2Facult. Med. Sci., Univ. Fukui, 3Depart. Phys.,
Kanazawa Univ.)

神経科学･感覚 / Neuroscience & Sensory systems
1Pos227* 線虫（C. elegans）嗅覚感覚神経細胞内の領域特異的なにおいに対する cGMP 応答
Compartmentalized cGMP responses to odor in Caenorhabditis elegans’ olfactory sensory neurons
Hisashi Shidara, Keita Ashida, Kohji Hotta, Kotaro Oka (Grad. Sch. Sci. and Tech., Keio Univ.)
1Pos228* C. elegans の低温適応における温度情報伝達の分子ロジック
Molecular logic for temperature signaling in cold tolerance of C. elegans

1Pos229

Tomoyo Ujisawa1, Misato Uda1, Akane Ohta1, Katsushi Arisaka2, Atsushi Kuhara1 (1Inst. for Integrative Neurobiology, Konan University., 2Dept.
of Physics and Astronomy, UCLA, U.S.A.)
蛍光温度計シートを用いた神経細胞の熱発生計測
Detection of Neural Thermogenesis with Fluorescent Thermometer Sheet
Mizuho Gotoh1,2,3, Kotaro Oyama1,4, Yuki Kawamura1, Hideki Itoh1,5, Shin’ichi Ishiwata1,6 (1Sch. Adv. Sci. Eng., Waseda Univ., 2Grad. Sch. Comp.

Human Sci., Tsukuba Univ., 3HIRI, AIST, 4Cell Physiol., Jikei Univ., 5Inst. Med. Biol., A*STAR, Singapore, 6WABIOS, Waseda Univ., Singapore)
1Pos230* 線虫の早期嗅覚順応における感覚・介在神経細胞の部分特異的可塑性
Compartmentalized modulations of sensory and interneurons for early adaptation in C. elegans
Keita Ashida, Hisashi Shidara, Kohji Hotta, Kotaro Oka (Keio University)
1Pos231* イベルメクチンによる α7 ニコチン性アセチルコリン受容体の分子内動態増大の発見
Discovery of the internal motion enhancement of α7 nAChR with Ivermectin
Tomoyuki Baba1, Keigo Ikezaki1, Hiroshi Sekiguchi2, Tai Kubo3, Yuji C. Sasaki1,2 (1Grad. Sch. Front. Sci., Univ. Tokyo, 2JASRI/SPring-8,
3MolprofRC/AIST)

1Pos232

ミミズ体壁刺激に関する慣れとその回復
Establishment and recovery of habituation by repeated tactile stimulus in earthworm
Yoshiichiro Kitamura1, Hitoshi Aonuma2, Hiroto Ogawa3, Kotaro Oka4 (1Dept. Math Sci. Phys, Kanto Gakuin Univ., 2Res Inst Elect Sci.,
Hokkaido Univ., 3Dept. Biol. Sci., Hokkaido Univ., 4Dept. Biosci Info, Keio Univ.)– S94 –

1Pos233

1Pos234

1Pos235

シャルコマリートゥース病の原因遺伝子の一つであるダイナミン２の変異は細胞の異常なアクチン動態とラメリポディア形成の減少をもたらす
Expression of a dynamin 2 mutant associated with Charcot-Marie-Tooth disease leads to aberrant actin dynamics and lamellipodia
formation
Hiroshi Yamada, Kinue Kobayashi, Yubai Zhang, Tetsuya Takeda, Kohji Takei (Dep. of Neurosci., Grad. Sch. of Med., Dent., and Pharm. Sci.,
Okayama Univ.)
ヒト苦味受容体の基質認識の分子機構研究
Ligand-induced structural changes of human bitter taste receptor
Mayu Hioki1, Masayo Iwaki1, Rei Abe-Yoshizumi1, Hiroo Imai2, Hideki Kandori1 (1Nagoya Inst. Tech., 2Primate Res. Inst.)
性ホルモンによる海馬神経シナプスの制御：オスとメスの性差
Effect of estrogen and androgen on hippocampal synapses : gender difference of male and female
Asami Kato1, Yasushi Hojo2, Yoshitaka Hasegawa1, Yusuke Hatanaka1, Suguru Kawato1,3,4 (1Grad. Sch. Univ. of Tokyo., 2Dept. Biochem.,SaitamaMed. Univ., 3Dept. of Urology, Juntendo Univ., 4Dept. of Urology, Teikyo Univ.)

光生物：視覚･光受容 / Photobiology: Vision & Photoreception
1Pos236

1Pos237ナトリウムポンプ型ロドプシンの光反応中間体の発色団構造
Structure of retinal chromophore of the photointermediates in sodium ion pump rhodopsin
Nao Nishimura1, Misao Mizuno1, Hideki Kandori2, Yasuhisa Mizutani1 (1Grad. Sch. Sci., Osaka Univ., 2Grad. Sch. Eng., Nagoya Inst. Tech.)
固体 NMR を用いたミドルロドプシンのレチナール結合ポケットの構造解析
Solid-state NMR structural study of retinal-binding pocket in middle rhodopsin
Izuru Kawamura1, Hayato Seki1, Arisu Shigeta1, Yoshiteru Makino1, Takashi Okitsu2, Akimori Wada2, Yuki Sudo3 (1Grad. Sch. Eng., Yokohama

Natl. Univ., 2Kobe Pharm. Univ., 3Okayama Univ.)
1Pos238* 酸性及び中性におけるナトリウムイオンポンプ KR2 のレチナール結合ポケットの固体 NMR 構造解析
Solid-state NMR analysis of retinal binding pocket structure of sodium ion pump, KR2, at acidic and neutral pH
Arisu Shigeta1, Shota Ito2, Takashi Okitsu3, Akimori Wada3, Keiichi Inoue2,4, Hideki Kandori2, Izuru Kawamura1 (1Graduate School of
1Pos239

Engineering, Yokohama National University, 2Nagoya Institute of Technology, 3Kobe Pharmaceutical University, 4JST PRESTO)
Na+/H+ハイブリッドポンプロドプシン KR2 における His30 の役割
Role of His30 in Na+/H+ Hybrid Pumping Rhodopsin KR2

Sahoko Tomida1, Shota Ito1, Rei Abe-Yoshizumi1, Keiichi Inoue1,2, Hideki Kandori1 (1Nagoya Inst. Tech. Kandori Laboratory, 2PRESTO, JST)
FTIR
分光法によって明らかになった光駆動内向きプロトンポンプの輸送機構
1Pos240*
Transport mechanism of light-driven inward proton pump revealed by FTIR spectroscopy
Shota Ito1, Sahoko Tomida1, Yoshitaka Kato1, Yurika Nomura1, Satoshi Tsunoda1, Keiichi Inoue1,2, Hideki Kandori1 (1Grad. Sch. Eng., Nagoya
1Pos241

Inst. Tech., 2PRESTO, JST)
H+ポンプ型ロドプシンの比較研究：H+ donor 残基の相互置換による検討
Replacements of “donor” residues in the light-driven H+-pump rhodopsins
Koki Nishiya1, Syogo Sasaki2, Jun Tamogami3, Takashi Kikukawa2, Tomoyasu Aizawa2, Naoki Kamo2, Makoto Demura2 (1Facu. Sci., Univ.

1Pos242

Hokkaido, 2Grad. Sch. Life Sci., Univ. Hokkaido, 3Facu. Phar., Univ. Matsuyama)
アセタブラリアロドプシン II のプロトン移動における D92 および C218 残基間の相互作用の役割
Role of the interaction between D92 and C218 in the proton transfer reaction in Acetabularia rhodopsin II
Jun Tamogami1, Takashi Kikukawa2, Keisuke Okawa1, Noboru Ohsawa3,4, Kohei Date1, Toshifumi Nara1, Makoto Demura2, Tomomi KimuraSomeya3,4, Mikako Shirouzu3,4, Shigeyuki Yokoyama3,5, Seiji Miyauchi6, Kazumi Shimono6, Naoki Kamo2 (1College Pharm. Sci., Matsuyama

Univ., 2Fac. Adv. Life Sci., Hokkaido Univ., 3RIKEN SSBC, 4RIKEN CLST, 5RIKEN Structural Biology Laboratory, 6Fac. Pharm. Sci., Toho Univ.)
1Pos243* 固体 NMR によるバクテリオロドプシンの暗順応状態における Tyr185 の構造解析
Structure of Tyr185 in dark-adapted bacteriorhodopsin as studied by solid-state NMR
Yuto Otani1, Arisu Shigeta1, Yoko Kebukawa1, Kensei Kobayashi1, Takashi Okitsu2, Akimori Wada2, Satoru Tuzi3, Akira Naito1,3, Izuru
1Pos244

Kawamura1 (1Grad. Sch. Eng, Yokohama Natl Univ., 2Kobe Pharm. Univ., 3Univ. of Hyogo)
光駆動ナトリウムポンプ KR2 の多量体形成に重要なアミノ酸残基
Oligomerization of light-driven sodium pump KR2 is important for ion transport activity
Rei Abe-Yoshizumi1, Shota Ito1, Mikihiro Shibata3, Keiichi Inoue1,2, Takayuki Uchihashi3, Hideki Kandori1 (1Nagoya Inst. Tech., 2JST PRESTO,
3Dept. Physics, Kanazawa Univ.)

1Pos245* Theoretical study on molecular mechanism of a light-driven ion transport of Halorhodopsin
Ryo Oyama, Shigehiko Hayashi (Grad. Sch. Sci., Kyoto Univ.)
1Pos246 Rhodobacter capsulatus PYP の複合体形成に伴う構造変化
Complex induced structural changes of Rhodobacter capsulatus Photoactive Yellow Protein
Yoichi Yamazaki, Yohei Shibata, Hironari Kamikubo (Grad. Sch. Mat. Sci. NAIST)
1Pos247 Rc-PYP の光依存的に形成する複数種の複合体解析
Analysis of light dependent multiple complex formation of Rc-PYP
Yohei Shibata, Yoichi Yamazaki, Keito Yoshida, Shoki Nakata, Hironari Kamikubo (Analysis of light dependent multiple complex formation of RcPYP)– S95 –

1Pos248* 同位体標識試料を用いた BLUF ドメインの水素結合環境の解明
Analysis of a hydrogen bonding network of the BLUF domain using isotope-labeled samples
Takashi Nagai1, Tatsuya Iwata1, Shota Ito1, Mineo Iseki2, Masakatsu Watanabe3, Masashi Unno4, Shinya Kitagawa1, Hideki Kandori1 (1Grad. Sch.

1Pos249

Eng., Nagoya Inst. Tech., 2Dept. Pharmacol., Toho Univ., 3Grad. Sch. for Creation of Photonics Indust., 4Dept. of Chem. and Applied Chem. Saga
Univ.)
ホタルオキシルシフェリン吸収・蛍光スペクトルにおける水和効果
Hydration effects on absorption and fluorescence spectra of firefly oxyluciferin
Miyabi Hiyama1, Yoshifumi Noguchi1, Hidefumi Akiyama1, Kenta Yamada2, Nobuaki Koga2 (1ISSP, Univ. Tokyo, 2Grad. Sch. Info. Sci., Nagoya
Univ.)

光生物：光合成 / Photobiology: Photosynthesis
光化学系 II におけるクロロフィル励起三重項状態の赤外分光解析
FTIR analysis on the localization of the excited triplet state of chlorophyll in photosystem II
Tatsuya Mitomi, Ryo Nagao, Takumi Noguchi (Grad. Sch. Sci., Nagoya Univ.)
1Pos251 光合成水分解反応におけるメタノール阻害機構の赤外分光解析
FTIR study on the mechanism of methanol inhibition in the S-state cycle of photosynthetic water oxidation
Haruna Yata, Tatsuki Shimizu, Takumi Noguchi (Grad. Sch. Sci., Nagoya Univ.)
1Pos252* Structure and Function of Novel Carbonyl-Carotenoid bound to Light-Harvesting Complex II from Transplastomic Lettuce
1Pos250

Nami Yamano1, Kentaro Ifuku2, Hideki Hashimoto1,3,5, Norihiko Misawa4, Ritsuko Fujii1,5 (1Grad. Sch. Sci., Osaka City Univ., 2Grad. Sch.
Biostudy, Kyoto Univ., 3Sch. Sci. Tech., Kwansei Gakuin Univ., 4Res. Inst. Biores. Biotech., Ishikawa prefectural Univ., 5OCARINA, Osaka City
Univ.)
1Pos253 偏光全反射赤外分光法による光合成水分解 Mn クラスター周辺のプロトン化構造の解析
Protonation structure around the water-oxidizing Mn cluster in photosystem II revealed by polarized ATR-FTIR spectroscopy
Shin Nakamura, Takumi Noguchi (Grad. Sch. Sci., Nagoya Univ.)
1Pos254 酸素発生系マンガンクラスターの配位水分子の化学的性質
Chemical properties of terminal water ligands of the Mn cluster
Hiroki Nagashima, Hiroyuki Mino (Grad. Sch. Sci. Nagoya Univ.)
1Pos255* 光化学系 II と阻害剤アジ化物イオンとの共結晶化と X 線結晶構造解析
Co-crystallization of photosystem II with an inhibitor NaN3, and its structural analysis

1Pos256

Shoya Tamaru1, Yasufumi Umena2, Jian-Ren Shen2 (1Graduate School of Natural Science and Technology, Okayama University., 2ReseachInstitute for Interdisciplinary Science, Okayama University.)
時間分解 EPR 法を用いた PSII 反応中心に生成する初期電荷分離構造・電子的相互作用の解析
Time resolved EPR study on orientations and electronic couplings of the primary charge-separated state in the PSII reaction center
Reina Minobe1, Masashi Hasegawa1, Shusuke Katagiri3, Takahiro Sakai2, Hiroki Nagashima2, Takashi Tachikawa1, Hiroyuki Mino2, Yasuhiro

1Pos257

1Pos258

1Pos259

Kobori1 (1Graduate School of Science, Kobe Univ., 2Graduate School of Science, Nagoya Univ., 3Graduate School of Science, Shizuoka Univ.)
FTIR 分光電気化学法を用いた光化学系 II における第一キノン電子受容体 QA の酸化還元電位計測
Measurement of the redox potential of the primary quinone electron accepter QA in photosystem II by FTIR spectroelectrochemistry
Ayaka Ohira, Ryo Nagao, Takumi Noguchi, Yuki Kato (Grad. Sch. Sci., Nagoya Univ.)
光化学系 II におけるキノン電子受容体の電子移動制御機構
Regulation mechanism of electron transfer between quinone electron acceptors in Photosystem II
Yosuke Nozawa, Takumi Noguchi (Division of Material Science, Graduate School of Science, Nagoya University)
Energy gap dependence for the exciton relaxation rate using Time-dependent renormalized Redfield theory
Akihiro Kimura (Graduate School of Science, Nagoya Univeristy)

光生物：光遺伝学･光制御 / Photobiology: Optogenetics & Optical Control
Photozipper-DNA 複合体平衡の定量的モデル
Quantitative modeling of the equilibria among Photozipper-DNA complexes
Yoichi Nakatani, Osamu Hisatomi (Grad. Sch. Sci., Osaka Univ.)
1Pos261 光制御型 bZIP モジュール Photozipper の構造変化の変異体解析
Mutational analyses of the conformational switching of a light-regulated bZIP module, Photozipper
Osamu Hisatomi (Graduate School of Science, Osaka University)
1Pos262 水晶微量天秤による光制御型 bZip モジュール photozipper の DNA 結合の解析
The DNA-binding of a light-regulated bZIP module, photozipper, analyzed by quartz crystal microbalanceSamu Tateyama, Osamu Hisatomi (Grad. Sch. Sci., Univ. Osaka)
1Pos263* 真正細菌のポンプ型ロドプシンの機能転換およびその分子メカニズムについての研究
Functional conversion of eubacterial pump rhodopsins and the investigation of the molecular mechanism

1Pos260

Yurika Nomura1, Keiichi Inoue1,2, Shota Ito1, Hideki Kandori1 (1Nagoya Inst. Tech., 2JST PRESTO)– S96 –

1Pos264

光駆動内向きプロトンポンプの発見
Natural light-driven inward proton pumpKeiichi Inoue1,2, Shota Ito1, Yoshitaka Kato1, Yurika Nomura1, Mikihiro Shibata3,4, Takayuki Uchihashi3,4, Satoshi Tsunoda1, Hideki Kandori1

1Pos265(1Grad. Sch. Eng., Nagoya Inst. Tech., 2JST PRESTO, 3Faculty Sci., Kanazawa Univ., 4Bio-AFM Frontier Research Center, Kanazawa Univ.)
電気生理実験により解析した光駆動型ナトリウムポンプの輸送機構
Transport mechanism of NaRs studied by electrophysiology measurement
Yuko Kozaki1, Satoshi Tsunoda1, Keiichi Inoue1,2, Rei Abe-Yoshizumi1, Hideki Kandori1 (1Nagoya Inst. Tech., 2JST PRESTO)

バイオインフォマティクス：構造ゲノミクス / Bioinformatics: Structural genomics
1Pos266

1Pos267

1Pos268

An index to select homologous sequences with the same functional region
Shoichiro Kato1, Hiroyuki Toh2, Wataru Nemoto1 (1Life Sci. & Eng., Grad. Sch. of Sci. & Eng, 2Dept. Biomed. Chem., Scl. of Sci. & Tech.,
Kwansei Gakuin Univ.)
GWAS データによる疾患関連複合体モデルの予測
Prediction of disease related supramolecule models using GWAS data
Toshiyuki Tsuji1,2, Atsushi Hijikata1, Takao Yoda1, Tsuyoshi Shirai1 (1Nagahama Institute of Bio-Science and Technology, 2MITA International
School)Predictions of cancer causing mutations that potentially affect GPCR-GPCR interaction
Shunsuke Fujishiro1, Vachiranee Limviphuvadh2, Sebastian Maurer-Stroh2, Yoshihiro Yamanishi3, Hiroyuki Toh4, Wataru Nemoto1 (1Life Sci. &Eng., Grad. Sch. of Sci. & Eng., Tokyo Denki Univ., 2BII, A*STAR., 3Med. Ins. of Bioreg., Kyushu Univ., 4Dept. Biomed. Chem., Sci. & Tech.,
Kwansei Gakuin Univ.)

ベクトル表現化したアミノ酸残基のマッチングによるタンパク質－リガンド結合予測
A new approach for protein-ligand binding predictions based on matching of vector-represented amino acid residues
Atsushi Hijikata, Masafumi Shionyu, Tsuyoshi Shirai (Nagahama Inst. Bio-Sci. Tech.)
1Pos270 β-Trefoil タンパクのフォールディングに重要な残基に関する残基間平均距離統計に基づく解析
Analysis of residues significant for folding of beta-trefoil proteins based on the inter-residue average distance statistics
Takuya Kirioka, Takeshi Kikuchi (Dept. of Bioinfo., Col. Life Sci., Ritsumeikan Univ.)
1Pos271* 多剤認識転写因子 LmrR における薬剤分子認識機構の計算化学的解析
Computational study on the mechanism of multidrug recognition by a transcriptional repressor LmrR
Kazuho Cryershinozuka, Tadaomi Furuta, Minoru Sakurai (Center for Biol. Res. & Inform., Tokyo Tech.)
1Pos272 Lysozyme superfamily のアミノ酸配列解析によるフォールディング領域予測
Folding region predictions by amino acid analysis of lysozyme superfamily proteins
Takuto Nakashima, Michiro Kabata, Takeshi Kikuchi (Dept. of Bioinfo., Col. Life Sci., Ritsumeikan Univ.)
1Pos273 二次構造順序の変化によって起こる蛋白質フォールドの多様化
Loop connectivity change drives protein fold divergence
1Pos269

1Pos274

Shintaro Minami1, George Chikenji2, Motonori Ota1 (1Grad. Sch. of Comput. Sci., Nagoya Univ., 2Grad. Sch. of Eng., Nagoya Univ.)
タンパク質の立体構造とアミノ酸配列間の疎水性の関係
The relationship between hydrophobicity in amino acid sequence and three-dimensional structure of a protein
Kohei Ohnishi, Takeshi Kikuchi (Dept. of Bioinf., Col. of Life Sci., Ritsumeikan Univ.)

数理生物学 / Mathematical biology
1Pos275

Role of interdomain communication in pacemaking circadian rhythm studied by a single molecule model of KaiC

Shota Hashimoto, Sumita Das, Masaki Sasai, Tomoki P. Terada (Dept. Comput. Sci. Eng., Grad. Sch. Eng., Nagoya Univ.)
1Pos276* A stochastic simulation study on the correlation between circadian oscillation and ATPase activity of KaiC hexamer
Sumita Das, Shota Hashimoto, Tomoki P. Terada, Masaki Sasai (Department of Computational Science and Engineering, Nagoya
University,Nagoya)
1Pos277 数理モデルによる心筋細胞の集団効果の解析
Community effect of cardiomyocytes in beating rhythms is ruled by stable cells
Tatsuya Hayashi1, Tetsuji Tokihiro1,2, Hiroki Kurihara2,3, Fumimasa Nomura4, Kenji Yasuda2,5 (1Grad. Sch. Math. Sci., The Univ. of Tokyo, 2JST,
1Pos278
1Pos279

1Pos280

CREST, 3Grad. Sch. Med., The Univ. of Tokyo, 4Inst. Biomat. Bioeng., Tokyo Medical and Dental Univ., 5Fac. Sci. Eng., Waseda Univ.)
Discreteness-induced transition in multi-body reaction systems
Yohei Saito, Yuki Sughiyama, Tetsuya Kobayashi (IIS, Univ. Tokyo)
Probability Eddy currents in stochastic gene expression dynamics in eukaryotes
Bhaswati Bhattacharyya1, Masaki Sasai1,2 (1Department of Computational science and engineering, Nagoya University, 2Department of Applied
Physics, Nagoya University)
集団増殖系における定常状態熱力学
Steady State Thermodynamics in Population Dynamics
Yuki Sughiyama, Tetsuya J. Kobayashi (IIS, Univ. Tokyo)– S97 –

1Pos281

1Pos282
1Pos283

1Pos284

上皮陥入過程における三次元多細胞動態の力学制御機構
Mechanical regulatory mechanism of 3D multicellular dynamics during epithelial invagination
Satoru Okuda, Mototsugu Eiraku (RIKEN Center for Developmental Biology)
Modeling folding of epithelial cell sheets
FuLai Wen1, YuChiun Wang2, Tatsuo Shibata1 (1RIKEN Quantitative Biology Center, 2RIKEN Center for Developmental Biology)
等方的なアクチンミオシン細胞骨格におけるモーター誘起応力に関する理論
Theory on motor-induced stress in an isotropic actomyosin cytoskeleton
Tetsuya Hiraiwa (Dept. Sci., Univ. Tokyo)
Phase-field simulations of the basic cell-cell effects of adhesion and chemoattractant on multi-cellular interaction
Daisuke Imoto1,5, Satoshi Sawai1,2,3, Shuji Ishihara4 (1Dept. Basic Sci., Grad. School of Arts and Sci., Univ. of Tokyo, 2Research Ctr for Complex

1Pos285

Systems Biology, Univ. of Tokyo, 3JST PRESTO, 4School of Sci. Eng., Meiji Univ., 5National Research Institute of Police Science)
様々な回転する自走粒子の集団運動
Collective motion of various kinds of rotating self-propelled particle
Ken Nagai1, Yutaka Sumino2, Chate Hugues3,4, Kazuhiro Oiwa5,6, Takuma Sugi7, Hideo Iwasaki8 (1Sch. Mater. Sci., JAIST, 2Dep. Appl. Phys.,
Tokyo Univ. Sci., 3CEA-Saclay, 4Beijing Comp. Sci. Res. Ctr., 5Adv. ICT Res. Inst., NICT, 6Grad. Sch. Sci., Univ. Hyogo, 7Mol. Neurosci. Res. Ctr.,
Shiga Univ. of Med. Sci., 8Sch. Adv. Sci. Eng., Waseda Univ.)

非平衡･生体リズム / Nonequilibrium state & Biological rhythm
1Pos286

蛍光分光法による時計タンパク質 KaiC の動的構造変化の解析
Spectroscopic characterization of the conformational change of the cyanobacterial clock protein KaiC
Atsushi Mukaiyama1,2, Jun Abe1, Yoshihiko Furuike1,2, Eiki Yamashita3, Takao Kondo4, Shuji Akiyama1,2 (1IMS, CIMoS, 2SOKENDAI, 3IPR,

4Naogya Univ.)
1Pos287* 高速原子間力顕微鏡によって明らかにする Kai タンパク質間の動的相互作用のリン酸化状態依存性
HS-AFM images reveal dynamic interaction between Kai proteins dependent on phosphorylation states of KaiC

Shogo Sugiyama1, Tetsuya Mori2, Takayuki Uchihashi1,3, Johnson Carl H.2, Toshio Ando1,3 (1Dept. of phys., Kanazawa Univ., 2Dept. of Biol. Sci.,
Univ. Vanderbilt, 3Bio-AFM FRC., Kanazawa Univ.)
1Pos288 生命システムの振動現象における頑健性と可塑性の互恵的関係
Reciprocity between robustness and plasticity in biological oscillators
Tetsuhiro S. Hatakeyama, Kunihiko Kaneko (Department of Basic Science, The University of Tokyo)
1Pos289* 自律的な振動運動を示す微小管リング状集合体
Mechanical Oscillation of Dynamic Microtubule Rings
Masaki Ito1, Kabir Arif Md. Rashedul2, Md. Sirajul Islam1, Daisuke Inoue2, Shoki Wada1, Kazuki Sada1,2, Akihiko Konagaya3, Akira Kakugo1,2(1Grad. of CSE, Hokkaido Univ., 2Fac. of Sci., Hokkaido Univ., 3DIS, TITECH)
1Pos290* キネシン駆動微小管のパターン形成と局所相互作用
Configuring Dynamic Patterns of Microtubules Driven by Kinesins
Sakurako Tanida1, Ken’ya Furuta2, Kaori Nishikawa1, Hiroaki Kojima2, Masaki Sano1 (1Graduate School of Science, The University of Tokyo,
2National Institute of Information and Communications Technology)
1Pos291* 離散的な相互作用を行う振動子ネットワークの解析
Analysis of nonlinear oscillator network with discrete interactions

Manami Ito1, Masahiro Takinoue1,2 (1Dept. Comput. Intell. Syst. Sci., Tokyo Tech., 2Dept. Comput. Sci., Tokyo Tech.)
1Pos292* 混み合い状況下におけるマイクロ粒子のラチェット輸送
Ratchet transport of microparticles in crowded conditions
Masayuki Hayakawa1, Yusuke Kishino2, Masahiro Takinoue1,2,3 (1Dept. of Comput. Intell. and Syst. Sci., Tokyo Tech., 2Dept. of Engineering,Tokyo Tech., 3Dept. of Computer Science, Tokyo Tech.)
1Pos293 「ゆらぎ」が創り出す「秩序構造」：細胞の混雑環境のモデリング
Fluctuation Creates Exotic Spatial-Order: Verification with a Simple Crowding Cellular-Model

1Pos294

Soutaro Oda1, Chwen-Yang Shew2, Kenichi Yoshikawa1 (1Faculty of Life and Medical Sciences, Doshisha University, 2Department of Chemistry,
CSI, City University of New York)
置かれた環境を感応する自発運動系：化学的非平衡性により駆動する生物らしさを示す実空間モデル
Smart response of chemically driven self-motile object: Real-world modeling
Shiho Sato1, Hiroki Sakuta2, Kenichi Yoshikawa1,2 (1Facul. Life Med. Sci., Univ. Doshisha, 2Grad. Sch. Life Med. Sci., Univ. Doshisha)

計測 / Measurements
1Pos295

1Pos296

Shot noise free number and brightness 解析法による生細胞内グルココルチコイド受容体二量体化過程の時空間分布解析
Spatio-temporal distribution analysis of glucocorticoid receptor dimerization in cells by shot noise free number and brightness analysis
Ryosuke Fukushima1, Jotaro Yamamoto2, Masataka Kinjo2 (1Grad. Sch. Life Sci., Hokkaido Univ., 2Faculty of Adv. Life Sci., Hokkaido Univ.)
蛍光異方性を用いた免疫センサの試作
Development of Fluorescence Anisotropy Immunosensor
Seiichi Suzuki, Sena Hasegawa, Maki Takagi, Takuya Ito, Toshinori Kojima (Faculty Sci. Tech., Seikei Univ.)– S98 –

1Pos297* 実時間選択的回収による免疫細胞の１細胞遺伝子発現解析
Single cell gene expression analysis of stimulated immune cells with real-time selection
Yumiko Tanaka1, Yoshitaka Shirasaki1,2, Mai Yamagishi1,2, Kaede Miyata1, Nobutake Suzuki1, Osamu Ohara2, Kazuyo Moro2, Sotaro Uemura1
1Pos298

1Pos299

1Pos300

1Pos301

1Pos302

1Pos303

1Pos304

1Pos305(1Grad. Sch. Sci., Univ. Tokyo, 2IMS., Riken)
カップ形状 AFM チップを用いた簡便な細胞間相互作用計測法の開発
Easy Measurement of Cell-Cell Interactions Using Cup-Shaped AFM Chip
Hyonchol Kim1, Ayana Yamagishi1, Miku Imaizumi2, Chikashi Nakamura1,2 (1Biomed. Res. Inst., AIST, 2Grad. Sch. Eng., Tokyo Univ. Agric.
Technol.)
アンルーフ法を用いた水溶液環境下における細胞内骨格のＡＦＭイメージング
An Unroofing Method to Observe the Cytoskeleton Directly at Molecular Resolution Using Atomic Force Microscopy
Eiji Usukura1, Akihiro Narita1, Akira Yagi2, Shuichi Ito2, Jiro Usukura1 (1Grad. Sch. Sci., Univ. Nagoya, 2Olympus Co., Ltd.)
原子間力顕微鏡による細胞機能と力学特性の単一細胞相関解析法
Atomic force microscopy for single-cell correlation analysis between cellular function and cell mechanical property
Ryosuke Tanaka1, Yoshikatsu Akiyama2, Jun Kobayashi2, Masayuki Yamato2, Okajima Takaharu1 (1Grad. Sch. Info. Tech. Univ. Hokkaido, 2Inst.
Adv. BioMed. Eng. Sci. Univ. Tokyo Women's Med.)
デジタルマイクロ流体技術によるデジタルバイオアッセイ
Digital bioassay in digital microfluidic platform
Ryohei Kobayashi1, Sadao Ota1,2, Hiroyuki Noji1,3 (1Appl. Chem., Grad. Sch. Eng., Univ. Tokyo, 2JST, PRESTO, 3ImPACT, JST)
左右両耳内部における脈波計測
Measurements of Pulse Waves in the Both Ears
Yoshitomi Morikawa (AIST)
ラボＸ線光源を用いたＸ線１分子動態観察
X-ray Single Molecule Observations using Laboratory X-ray Generator
Keigo Ikezaki1, Ken Matsubara1, Yuhuku Matsushita1, Jae-won Chang1, Hiroshi Sekiguchi2, Yuji Sasaki1 (1University of Tokyo, 2Spring-8/JASRI)
MALDI 法におけるマトリクスの分光学的解析
Spectroscopic analysis of matrices in ionization process of matrix-assisted laser desorption/ionization
Noritaka Masaki, Shigetoshi Okazaki (Dept. Med. Spec., Hamamatsu Univ. Sch. Med.)HbA1c の蛍光相関分析に及ぼすヘモグロビン光吸収の影響
Effects of hemoglobin absorption on fluorescence correlation analysis for HbA1c
Atsushi Matsuo, Yasutomo Nomura, Mayuka Chiba, Misaki Naraoka (Maebashi Institute of technology)

バイオイメージング / Bioimaging
1Pos306* 高速原子間力顕微鏡を用いた癌細胞の核膜孔動態の可視化
High-speed atomic force microscopy visualization of the nuclear pores dynamics in cancer cells
Mahmoud Shaaban Mohamed1,2,3, Yosuke Kikuchi4, Watanabe-Nakayama Takahiro2, Azuma Taoka4, Akiko Kobayashi1,2,3, Masaharu
Hazawa1,2,3, Noriyuki Kodera2, Takayuki Uchihashi2, Yoshihiro Fukumori4, Toshio Ando2, Richard Wong1,2,3 (1Cell-Bionomics Research Unit,
Kanazawa University, 2Bio-AFM Frontier Research Center, Kanazawa University, 3Lab of Mol. Cell Biol. Institute of Science and Engineering,
1Pos307

1Pos308

Kanazawa University, 4Institute of Science and Engineering, Kanazawa University)
高速 AFM による抗体分子の動的観察
The dynamic behaviors of antibody molecules
Yoko Kawamoto-Ozaki, Norito Kotani, Kumaresan Ramanujam, Aya Murakami, Takashi Morii, Takao Okada (Research Institute of Biomolecule
Metrology Co.,Ltd.)
高速 AFM によるタンパク質の動的観察に向けた立体パターン基板の作製
Fabrication of 3D-patterned Substrate as a Platform for HS-AFM Observation of Protein Dynamics
Akane Goto1, Shin’nosuke Yamanaka1, Mikihiro Shibata1,2, Takayuki Uchihashi1,2, Noriyuki Kodera1,2, Toshio Ando2 (1Dept. of phys., Kanazawa

1Pos309

Univ., 2Bio-AFM FRC)
高速スイッチング蛍光タンパク質と改良された SPoD-ExPAN による超解像イメージング
Superresolution imaging of live cells by fast photoswitching fluorescent protein and improved SPoD-ExPAN microscopy
Tetsuichi Wazawa1,2, Yoshiyuki Arai1,2, Tomoki Matsuda1,2, Hiroki Takauchi1, Yoshinobu Kawahara1,2, Takashi Washio1,2, Takeharu Nagai1,2

1Pos310(1ISIR, Osaka Univ., 2CREST, JST)
生細胞核内における INO80 クロマチン再構成複合体の 1 分子イメージング
Single-molecule imaging of the INO80 chromatin remodeling complex in the living cell nucleus

Yuma Ito1, Masahiko Harata2, Kumiko Sakata-Sogawa1, Makio Tokunaga1 (1Sch. Life Sci. Tech., Tokyo Inst. Tech., 2Grad. Sch. Agr. Sci., Tohoku
Univ.)
1Pos311* アロディニア特異的な痛みに対する鎮痛薬評価系の確立に関する fMRI 研究
An fMRI study to establish an evaluation system of analgesic agents on allodynia-specific pain
Naoya Yuzuriha1, Sosuke Yoshinaga1, Mitsuhiro Takeda1, Hiroshi Sato2, Hiroaki Terasawa1 (1Fac. Life Sci., Kumamoto Univ., 2Bruker Biospin
K.K.)– S99 –

1Pos312
1Pos313

CLIP-170 phosphorylation mediates repositioning of microtubule-organizing center during T cell activation
Wei Ming Lim, Yuma Ito, Makio Tokunaga, Kumiko Sakata-Sogawa (Sch. Life Sci. Tech., Tokyo Inst. Tech.)
核小体構成タンパク質動態の１分子イメージング定量解析
Single molecule imaging and quantitative analysis of Nucleolar-localized protein dynamics
Daiki Matsumoto1, Yuma Ito1, Noriko Saitoh2, Kumiko Sakata-Sogawa1, Makio Tokunaga1 (1Sch. Life Sci. Tech., Tokyo Inst. Tech., 2IMEG,

1Pos314

Kumamoto Univ.)
高速超解像光学顕微鏡を用いた出芽酵母の膜交通の観察
Observations of the membrane traffic in living yeast cells via the high-speed super-resolution optical microscope

Daisuke Miyashiro1, Kazuo Kurokawa1, Akihiko Nakano1,2 (1Riken, RAP, 2University of Tokyo, Dep. Bio. Sci.)
1Pos315* 新規微分干渉顕微法を用いた生細胞ヘテロクロマチンにおける物質密度のイメージング“Density” imaging of heterochromatin in live mouse cells using OI-DIC microscopy
Ryosuke Imai1,2, Tadasu Nozaki1, Tomomi Tani3, Kayo Hibino1,2, Michael Shribak3, Kazuhiro Maeshima1,2 (1Natl. Inst. of Genet., 2SOKENDAI,
3MBL, Woods Hole, USA)

1Pos316

1Pos317

線虫 C. elegans 胚発生における細胞動態の個体差定量解析
Quantitative analysis of variability of cellular dynamics in C. elegans embryogenesis
Yusuke Azuma, Shuichi Onami (RIKEN QBiC)
神経分化時における神経細胞内温度イメージング
Imaging of intracellular temperature in PC12 cell nerve differentiation
Masaki Kinoshita1,2, Kohki Okabe3,4, Hisashi Tadakuma2, Yoshie Harada1,2,5 (1Grad. Sch. Bio., Kyoto Univ., 2iCeMS, Kyoto Univ., 3Grad. Sch.

Pharm, Tokyo Univ., 4PRESTO, JST, 5IPR, Osaka Univ.)
1Pos318* ヒト免疫応答の 1 細胞実時間イメージングによるアレルギー診断の可能性
Potential allergy diagnosis by real-time single-cell secretion imaging of human immune response
Kaede Miyata1, Yoshitaka Shirasaki1,2, Nobutake Suzuki1, Hiroki Kabata3, Mai Yamagishi1,2, Osamu Ohara2, Koichi Fukunaga3, Kazuyo Moro2,

1Pos319

Sotaro Uemura1 (1Department of Bological Sciences, Graduate school of Tokyo, 2Institute of Physical and Chemical Research, IMS., 3Division of
Pulmonary Medicine, Keio University)
ソフトウェア「閻魔」と EMC アルゴリズムを用いたタンパク質 3 次元電子密度分布の再構成：XFEL-CXDI 実験を想定したシミュレーション
Reconstruction of three-dimensional structures of a protein with software ENMA and EMC algorithm: A simulation for XFEL-CXDI
experiment
Takashi Yoshidome1, Yuki Sekiguchi2,3, Tomotaka Oroguchi2,3, Masayoshi Nakasako2,3, Mitsunori Ikeguchi4 (1Dep. of Appl. Phys., Tohoku Univ.,
2Fac. of Sci. and Tech., Keio Univ., 3RIKEN SPring-8 Center, 4Grad. Sch. of Med. Life Sci. Yokohama City Univ.)

1Pos320X 線自由電子レーザーを用いた低温コヒーレントＸ線回折イメージングによるシアノバクテリアの三次元構造解析
Three-dimensional structure of a cyanobacterium visualized by cryogenic coherent X-ray diffraction imaging using X-ray free-electron
laser
Amane Kobayashi1,2, Yuki Sekiguchi1,2, Koji Okajima1,2, Tomotaka Oroguchi1,2, Masayoshi Nakasako1,2, Yayoi Inui3, Takeshi Hirakawa3,
Sachihiro Matsunaga3, Masaki Yamamoto2 (1Sci. Tech., Keio Univ., 2RIKEN SPring-8 Center, 3Sci. Tech., Tokyo Univ. Sci.)

第2 日目（11 月26 日（土））／Day 2（Nov. 26 Sat.）大会議室 101 ＋102、多目的ホール／Conference Room 101+102, Multi-Purpose Hall

蛋白質：構造 / Protein: Structure
2Pos001* カルシウム依存的な鞭毛運動の制御に関わるタンパク質カラクシンの構造解析
Structural analysis of calaxin, calcium-dependent flagellar movement regulator
Tomoki Shojima1, Feng Hou1, Yusuke Takahashi1, Masahiko Okai1, Katsutoshi Mizuno2, Kazuo Inaba2, Takuya Miyakawa1, Masaru Tanokura1
2Pos002(1Grad. Sch. Agr. Life Sci., Univ. Tokyo, 2Shimoda Marine Research Center, Univ. Tsukuba)
Porphyromonas gingivalis の T9SS によって分泌される PGN_0123 の構造
Structure of PGN_0123, a Type IX secretion substrate of Porphyromonas gingivalis

2Pos003

Yusuke Handa1, Keiko Sato2, Koji Nakayama2, Katsumi Imada1 (1Grad. Sch. Sci. Osaka Univ., 2Grad. Sch. Biomedical Sci., Univ. Nagasaki)
時計タンパク質 KaiC のリン酸化状態と脱リン酸化状態における構造上の差異
Structural Differences between Phosphorylated and Dephosphorylated States of Clock Protein KaiC
Yoshihiko Furuike1, Jun Abe1, Eiki Yamashita3, Takao Kondo4, Shuji Akiyama1,2 (1Research Center of Integrative Molecular Systems (CIMoS),
Institute for Molecular Science (IMS), 2Department of Functional Molecular Science, SOKENDAI (The Graduate University for Advanced Studies),
3Institute for Protein Research, Osaka University, 4Graduate School of Science, Nagoya University)

2Pos004
2Pos005

Structural characterization of Hsp104 from a thermophilic fungus, Chaetomium thermophilum
Yosuke Inoue (Tokyo University of Agriculture and Technology)
圧力応答を示す YFP 挿入変異体の高圧下での結晶構造
Crystal structure of a pressure sensitive YFP mutant under high pressure
Mika Tsujii1, Takayuki Nagae2, Keiko Yoshizawa3, Tomonobu Watanabe3, Masahiro Nishiyama4, Nobuhisa Watanabe2, Tatsuya Kawaguchi1,
Katsumi Imada1 (1Grad. Sch. Sci., Univ. Osaka, 2SRRC. Nagoya Univ., 3QBiC, Riken., 4Grad. Sch. Sci., Univ. Kyoto)– S100 –2Pos006

Crystallization of Hepatitis B virus Core Protein in genotype C
Katsumi Omagari (Nagoya City University)
2Pos007* 巨大タンパク質会合体ヘモシアニンの多孔質性結晶を用いた生体分子の包摂
Encapsulation of biomacromolecules into porous crystal of a huge protein complex hemocyanin
Asuka Matsuno1, Ye Yuxin2, Yuki Ohnishi2, Akira Kitamura1,2, Masataka Kinjo1,2, Satoshi Abe4, Takafumi Ueno4, Yoshikazu Tanaka1,2,3, Min
Yao1,2 (1Graduate School of Life Science, Hokkaido University, 2Faculty of Advanced Life Science, Hokkaido University, 3JST, PRESTO,
4Department of Life Science and Technology, Tokyo Institute of Technology)

2Pos008

蛋白質結晶中の分子間静電相互作用計算
Calculation of inter-molecular electrostatic interactions in protein crystals
Takuya Takahashi1, Shigeru Endo2, Masanori Ootaki3, Yoko Sugawara2 (1Bioinfo., Coll. Life. Sci., Ritsumeikan Univ., 2Dept. Physics, Sch. Sci.,

2Pos009

Kitasato Univ., 3Dep. Pharmacology, St. Marianna Univ. School of Medicine)
X 線自由電子レーザー（XFEL）回折像からの生体分子三次元構造の復元プログラムの開発
Development of 3D reconstruction program for coherent diffraction patterns obtained by XFEL
Miki Nakano1, Osamu Miyashita1, Slavica Jonic2, Atsushi Tokuhisa1, Daewoon Nam3, Yasumasa Joti4, Changyong Song3, Florence Tama1,5(1RIKEN AICS, 2IMPMC, Sorbonne University - CNRS UMR 7590, UPMC Univ. Paris 6, MNHN, IRD UMR 206, 3POSTECH, Korea, 4JASRI

2Pos010

2Pos011

XFEL, 5Grad. Sch. Science, Nagoya Univ.)
Hsp90 の構造変化に関する理論的研究
Theoretical study of a conformational change in Hsp90
Kazutomo Kawaguchi, Hidemi Nagao (Inst. Sci. Eng., Kanazawa Univ.)
Building a database of 3D biological shapes for the interpretation of XFEL diffraction patterns

Sandhya Tiwari1, Osamu Miyashita1, Florence Tama1,2 (1Riken Advanced Institute for Computational Science, 2Nagoya University)
胆汁酸輸送体の分子動力学シミュレーション
2Pos012
Molecular dynamics simulation of the bile acid transporter
Shin-ichiro Tasaki, Ryunosuke Yoshino, Yoshitaka Moriwaki, Kentaro Shimizu, Tohru Terada (Grad. Sch. of Agri. Life Sci., Univ. of Tokyo)
2Pos013* フレキシィボタンパク質－タンパク質ドクイン： PaCS−MD の応用
Flexible-Body Protein-Protein Docking: an Application of Parallel Cascade Selection Molecular Dynamics
2Pos014

Duy P. Tran1, Akio Kitao1,2 (1UTokyo, GSFS, 2UTokyo, IMCB)
分子動力学シミュレーションを用いた Hras-GTP/GDP 複合体と溶媒水との水素結合の動きの解析
Analysis of dynamics of hydrogen bond between the solvent water and the Hras-GTP/GDP complexes by molecular dynamics simulations
Takeshi Miyakawa1, Ryota Morikawa1, Masako Takasu1, Kimikazu Sugimori2, Kazutomo Kawaguchi2, Hidemi Nagao2 (1Tokyo Univ. of Pharm. &2Pos015

Life Sci., 2Kanazawa Univ.)
分子動力学シミュレーションによる抗 HIV 中和抗体 PG16 の CDR-H3 における構造剛性の解析
Molecular dynamics study of the structural rigidity of CDR-H3 of anti-HIV neutralizing antibody PG16
Ryo Kiribayashi1, Hiroko Kondo1, Daisuke Kuroda2, Toru Saito1, Jiro Kohda1, Akimitsu Kugimiya1, Yasuhisa Nakano1, Yu Takano1 (1Hiroshima

2Pos016City Univ., 2Showa Univ.)
Molecular dynamics simulations of the basic amyloidogenic unit of IAPP
Richa Tambi1, Satoshi Kosuda1, Gentaro Morimoto2, Makoto Taiji2, Yutaka Kuroda1 (1Tokyo University of Agriculture and Technology,
2Quantitative Biology Center, RIKEN)

2Pos017

レプリカ交換モンテカルロ SAAP3D 法による C-ペプチドと Trp ケージの分子シミュレーション
Molecular simulation of C-peptide and Trp-cage by SAAP3D-REMC method
Michio Iwaoka, Natsuki Babe, Yuya Shoji (Tokai University, Department of Chemistry)

2Pos018

REST 法による TRP-cage のフォールディングシミュレーション
In silico folding simulation of Trp-cage using the REST method and its variants

2Pos019

Motoshi Kamiya1, Yuji Sugita1,2,3 (1AICS, RIKEN, 2RIKEN, 3QBiC, RIKEN)
タンパク質のフォールディング過程における階層性と不均一性の分子論的起源
Molecular origin of heterogeneity and hierarchy behind protein folding

2Pos020

Toshifumi Mori1,2, Shinji Saito1,2 (1IMS, 2SOKENDAI)
プロリン型人工アミノ酸を含むペプチドの分子動力学計算
Molecular dynamics simulation of peptide oligomers bearing the proline-type artificial amino acid

Yuko Otani1, Satoshi Watanabe1, Akio Kitao2, Tomohiko Ohwada1 (1Grad. Sch. Pharm. Sci., Univ. Tokyo, 2IMCB, Univ. Tokyo)
Theoretical study of diffusion of plastocyanin with Langevin equation
Makoto Wada, Satoshi Nakagawa, Shogo Kinoshita, Kurniawan Isman, Kouichi Kodama, Kazutomo Kawaguchi, Hidemi Nagao (Nat. Sci.
Kanazawa Univ.)
2Pos022* HIV-1 protease の触媒的加水分解反応に関する理論的研究
Theoretical study on catalytic hydrolysis of HIV-1 protease
Masahiro Kaneso, Shigehiko Hayashi (Grad. Sch. Sci., Kyoto. Univ.)

2Pos021– S101 –蛋白質：構造機能相関 / Protein: Structure & Function
2Pos023

ホタルルシフェラーゼの全原子を考慮した発光基質オキシルシフェリンの光吸収のｐ H 依存性の定量解析
Quantitative Analysis of pH Effect on Absorption Peaks of Oxyluciferin by Considering All Atoms of Firefly Luciferase
Hironori Sakai1, Itsuki Kaji2, Naohisa Wada2 (1Insti. of Fluid Science, Tohoku Univ., 2Food Life Sciences, Toyo Univ.)

2Pos024

糖転移酵素の糖選択性とタンパク質認識に関わるアミノ酸の解析
Sequence and structure analysis of glycosyltransferases for understanding the sugar selectivity and target recognition mechanisms

2Pos025

Go Miyasaka1, Kenji Etchuya2, Yuri Mukai1,2 (1Sch. Sci. & Tech., Meiji Univ., 2Grad. Sch. Sci. & Tech., Meiji Univ.)
アルカン合成関連酵素の機能発現における保存部位の役割
Alanine scanning mutagenesis reveals functional roles of conserved residues in an enzyme for alkane biosynthesis

2Pos026

Masashi Nomura, Hisashi Kudo, Yuuki Hayashi, Munehito Arai (Dept. Life Sci., Univ. Tokyo)
示差走査型蛍光定量法を用いたアルドケト還元酵素の化合物選択性の評価
Evaluation of compound selectivity of aldo-keto reductases using differential scanning fluorimetry
Kabir Aurangazeb1, Satoshi Endo2, Naoki Toyooka3, Mayuko Fukuoka1, Kazuo Kuwata1,4, Yuji Kamatari5 (1United Grad. Sch. Drug Dis. Med. Inf.

Sci., Gifu Univ., 2Lab. Biochem., Gifu Pharm. Univ., 3Grad. Sch. Sci. Tech. Res., Univ. Toyama, 4Grad. Sch. Med., Gifu Univ., Gifu Univ., 5Life Sci.
Res. Ctr.)
2Pos027 Unique mechanism for broad substrate specificity of human MTH1
Shaimaa Ali, Teruya Nakamura, Keisuke Hirata, Mami Chirifu, Shinji Ikemizu, Yuriko Yamagata (Grad. Sch. Pharmaceut. Sci., Kumamoto Univ.)
2Pos028* ラン藻由来アルカン合成関連酵素の構造機能解析
Structural and functional analysis of a cyanobacterial enzyme for alkane biosynthesis
Hisashi Kudo1, Ryota Nawa2, Yuuki Hayashi1,2, Munehito Arai1,2 (1Dept. Life Sci., Univ. Tokyo., 2Dept. Pure & Applied Sci., Univ. Tokyo.)
2Pos029

クジラミオグロビンの分子進化
Tracing evolution of whale myoglobin by resurrecting ancient proteins
Yasuhiro Isogai1, Hiroshi Imamura2, Setsu Nakae3, Tomonari Sumi4, Ken-ichi Takahashi3, Taro Nakagawa3, Antonio Tsuneshige5, Tsuyoshi Sirai3(1Dept. Biotech., Toyama Pref. Univ., 2Biomedical Res. Inst., AIST, 3Dept. Comp. Bio-Sci., Nagahama Inst. Bio-Sci. Tech., 4Dept. Chem., Okayama

2Pos030

2Pos0312Pos032

2Pos033

Univ., 5Nano-Tech. Center, Hosei Univ.)
MEK1 リン酸化に伴う構造変化
Structual dynamics of MEK1 activation through phosphorylation
Minami Ando, Kei Moritsugu, Akinori Kidera (Grad. Sch. of Med. Life Sci., Yokohama City Univ.)
アロステリーの概念拡張に向けて：トロンビンのアロステリック制御・再訪
Toward Expanding the Concept of Allostery: Thrombin Allosteric Regulation, Revisited
Ikuo Kurisaki1,2, Masayoshi Takayanagi1,2, Barberot Chantal1,2, Masataka Nagaoka1,2 (1Grad. Sch. Info. Sci. Univ. Nagoya, 2JST-CREST)
サルコシン酸化酵素の反応生成物は４つの水チャネルの 1 つを選択的に移動する：平均力ポテンシャルによる検証
Potential of mean force shows that the reaction product of sarcosine oxidase selectively exits from one of four water channels
Takami Saito1, Go Watanabe2, Daisuke Nakajima2, Haruo Suzuki2, Shigetaka Yoneda2 (1Grad. Sch. Sci., Kitasato Univ., 2Sch. Sci., Kitasato Univ.)
Characterizing NO diffusion in nitrite reductase: nitric oxide reductase complex
Po-hung Wang1, Kenta Yamada1, Takehiko Tosha2, Yoshitsugu Shiro2,3, Yuji Sugita1,4,5,6 (1RIKEN Theoretical Molecular Science Laboratory,
2RIKEN SPring-8 Center, 3Graduate School of Life Science, University of Hyogo, 4RIKEN Advanced Institute for Computational Science, 5RIKEN

Quantitative Biology Center, 6RIKEN iTHES)
2Pos034* 二段階緩和モード解析による蛋白質シミュレーションの動的解析
Dynamical analysis of protein simulations by using two-step relaxation mode analysis
2Pos035

2Pos036

2Pos037

2Pos038

Naoyuki Karasawa1, Ayori Mitsutake1,2, Hiroshi Takano1 (1Grad. Sch. Sci. Technol., Keio Univ., 2JST, PRESTO)
MSES 法による EGFR キナーゼドメイン活性化の全原子構造解析
Structural basis for activation of EGFR kinase domain at atomistic resolution revealed by multiscale enhanced sampling
Kei Moritsugu1, Tohru Terada2, Akinori Kidera1 (1Grad. Sch. of Med. Life Sci., Yokohama City Univ., 2Grad. Sch. of Agri. and Life Sci., Univ. ofTokyo)
生物学的レアイベントを再現する効率的構造サンプリング手法の開発
Developments of conformational sampling methods for reproducing biologically rare events of proteins
Ryuhei Harada, Yasuteru Shigeta (Center for Computational Sciences, University of Tsukuba)
タンパク質の長時間シミュレーションに関する緩和モード解析
Relaxation mode analysis for long time simulations of proteins
Ayori Mitsutake1,2, Hiroshi Takano1 (1Dept. Physics, Keio Univ., 2JST, PRESTO)
Molecular basis for Hsp104-mediated prion propagation in yeast

Yoshiko Nakagawa1,2, Hideki Taguchi3, Motomasa Tanaka1 (1RIKEN Brain Science Institute, 2Tokyo Institute of Technology, 3Institute of
Innovative Research, Tokyo Institute of Technology)
2Pos039* HSP70 のフタの構造動態は基質結合にいかに関与するか？
How the lid exploits its structure dynamics in grasping the substrate in HSP70
Kohei Umehara1, Naoya Tochio2, Miho Hoshikawa1, Shoji Ueki3, Shin-ichi Tate1,2 (1Dept. Math. and Life Sci., Hiroshima Univ., 2RcMcD,
Hiroshima Univ., 3Kagawa Sch. Pharm. Sci., Tokushima bunri Univ.)– S102 –2Pos040

New insights into high molecular weight complex formation of 2-Cys peroxiredoxin and its chaperone function
Takamitsu Haruyama1, Takayuki Uchihashi1,2, Noriyuki Kodera1, Toshio Ando1, Hiroki Konno1 (1Bio-AFM FRC, Coll. Sci. & Eng., Kanazawa

2Pos041
2Pos0422Pos043

2Pos044

Univ., 2Coll. Sci. & Eng., Kanazawa Univ.)
Spectral characteristics of chimeric channelrhodopsins implicate the molecular identity involved in desensitization
Alemeh Zamani, Toru Ishizuka, Hiromu Yawo (Tohoku University)
タンパク質の構造と機能の相関を利用した Channelrhodopsin と MtrF の戦略的立体構造モデリング
Strategic modeling of channelrhodopsins and MtrF based on the correlation between protein structures and functions
Hiroshi C. Watanabe1,2, Yuki Yamashita2, Marcus Elstner3, Hiroshi Ishikita1,2 (1UTokyo, RCAST, 2UTokyo, School of Engineering, 3Karlsruhe
Institute of Technology)
機械受容チャネル MscL のゲーティングにおいてメカノセンサーとゲートは密接に連動する
Mechanosensor and the gate are tightly coupled in the mechano-gating of the bacterial mechanosensitive channel MscL
Yasuyuki Sawada1, Takeshi Nomura2, Masahiro Sokabe1 (1Mechanobiology Lab Nagoya Univ. Grad. Sch. Med., 2Physical Therapy Grad. Sch.
Health Sciences Kyushu Nutrition Welfare Univ.)
電位依存性プロトンチャネル VSOP/Hv1 における亜鉛イオンの結合様式の解析
A detailed analysis of the binding mode of a zinc ion to the voltage-gated proton channel VSOP/Hv1
Hiroko X. Kondo1,2, Masayo Iwaki3, Yusuke Kanematsu1, Matsuyuki Shirota2,4,5, Yasushige Yonezawa6, Kengo Kinoshita2,5,7, Hideki Kandori3,
Yu Takano1 (1GSIS, Hiroshima City Univ., 2GSIS, Tohoku Univ., 3Grad. Sch. Eng, Nagoya Inst tech, 4Grad. Sch. Med., Tohoku Univ., 5ToMMo,

Tohoku Univ., 6IAT, Kinki Univ., 7IDAC, Tohoku Univ.)
2Pos045 Molecular dynamics investigation of the full maltose transporter with and without the maltose binding protein MalE
WeiLin Hsu, Tadaomi Furuta, Minoru Sakurai (Center for Biol. Res. & Inform., Tokyo Tech.)
2Pos046* Binding and conformational dynamics of TOM20 and mitochondrial targeting signals using computational methods

2Pos047

2Pos048

Arpita Srivastava1, Osamu Miyashita2, Florence Tama1,3 (1Dept. Phys., Sch. Sci., Nagoya Univ., 2RIKEN Adv. Inst. Comp. Sci., 3ITbM, Nagoya
Univ.)
細胞間接着結合における α カテニン分子の力-構造-機能ダイナミクス
Mechanical, structural and functional dynamics of α-catenin molecule at intercellular adherens junctions
Koichiro Maki1,2, Taiji Adachi1,2 (1Institute for Frontier Medical Sciences, Kyoto University, 2Department of Micro Engineering, Graduate Schoolof Engineering, Kyoto University)
高速 AFM によるバクテリアコンデンシン複合体 MukBEF の構造動態の研究
Structural dynamics of bacterial condensin complex MukBEF studied by HS-AFM
Hironori Yoneda1, Kouichi Yano2, Noriyuki Kodera3,4, Kenta Yagi1, Hironori Niki2, Toshio Ando1,3,5 (1Div. of Math & Phys. Sci., Grad. Sch. of

2Pos049

Nat. Sci. & Tech., Kanazawa Univ., 2Natl. Inst. of Genet., 3Bio-AFM FRC, Inst. of Sci. & Eng., Kanazawa Univ., 4PRESTO, JST, 5CREST, JST)
高速 AFM によるダイナミン 1－アンフィファイジン複合体の動態観察
High-Speed AFM imaging of dynamics of Dynamin1-Amphiphysin1 complexes
Daiki Ishikuro1, Tetsuya Takeda3, Toshiya Kozai1, Yusuke Kumagai1, Kaho Seyama3, Huiran Yang3, Hiroshi Yamada3, Takayuki Uchihashi1,2,

Toshio Ando1,2, Kohji Takei3 (1College of Science and Engineering, Kanazawa Univ., 2Bio-AFM FRC, Kanazawa Univ., 3Graduate School of
Medicine, Dentistry and Pharmaceutical Sciences, Okayama Univ.)
2Pos050* 過飽和条件下におけるタンパク質分子内二次構造変化と芳香族アミノ酸立体配置
Internal Secondary Structural Changes and Aromatic Rings Conformation of Protein under Supersaturated Condition
Yufuku Matsushita1, Hiroshi Sekiguchi2, Jae-won Chang1, Keigo Ikezaki1, Masaki Nishijima3, Daizo Hamada4, Yuji Goto5, Yuji Sasaki1,2 (1Grad.
2Pos051

Sch. Front. Sci., Univ. Tokyo, 2JASRI/SPring-8, 3UIC, Osaka Univ., 4Int. Res. Cent., Kobe Univ., 5IPR, Osaka Univ.)
二次元蛍光寿命相関分光法による脂質膜上シトクロム c の環境に依存した構造ゆらぎの研究
Study of environment dependent dynamics of cytochrome c on a lipid membrane by 2D fluorescence lifetime correlation spectroscopy
Miyuki Sakaguchi1, Masaru Yamanaka2, Shun Hirota2, Kunihiko Ishii1,3, Tahei Tahara1,3 (1Molecular Spectroscopy Lab. RIKEN, 2NAIST Graduate
School of Materials Science, 3RIKEN Center for Advanced Photonics)

蛋白質：物性 / Protein: Property
2Pos052* 一分子蛍光分光測定により明らかになったユビキチンの変性状態の不均一性とゆっくりとしたダイナミクス
Significant Heterogeneity and Slow dynamics of the Unfolded Ubiquitin Detected by Single-Molecule Fluorescence Spectroscopy
Masataka Saito1,2, Kamonprasertsuk Supawich1, Keiichiro Kushiro3, Madoka Takai3, Eric H.-L. Chen4, Po-Ting Chen4, Rita P.-Y. Chen4, Hiroyuki
2Pos053

2Pos054

Oikawa1,2, Satoshi Takahashi1,2 (1Grad. Sch. Sci., Tohoku Univ., 2IMRAM, Tohoku Univ., 3Sch. Eng., Univ. Tokyo., 4IBC, Academia Sinica)
高活性型 DHFR 変異体の構造揺らぎの NMR 解析
NMR analysis of structural fluctuation of a highly active mutant of Escherichia coli dihydrofolate reductase
Takuro Nobe, Yuki Hayashi, Munehito Arai (Dept. Life Sci., Univ. Tokyo)
C-ペプチド領域をもたないウシ膵臓インスリンの二本鎖酸化的フォールディング経路
Double-chain oxidative folding pathways of bovine pancreatic insulin without C-peptide region

Kenta Arai1, Toshiki Takei1,2, Reina Shinozaki1, Yuya Asahina2, Hironobu Hojo2, Michio Iwaoka1 (1Tokai Univ., 2Inst. for Prot. Res., Osaka Univ.)
2Pos055* 圧力ジャンプ FTIR 法を用いた β ラクトグロブリンのフォールディング反応解析
A pressure-jump FTIR study of the folding reaction of β-Lactoglobulin
Satoshi Hayakawa, Tsubasa Yamamoto, Minoru Kato (Dept. Applied Chemistry, Ritsumeikan Univ.)– S103 –2Pos056

700 MPa 高圧下で観測された蛍光蛋白質 Akane families の特異な蛍光挙動
Unique properties of a GFP-like protein Akane families, observed under 700 MPa high-pressure
Akihiro Maeno1, Yuko Kato2, Mitsuru Jimbo3, Kei Amada4, Kazuyuki Akasaka5 (1Chem., Kansai Med. Univ., 2Electro. Res. Lab., Fukuoka Inst.

Tech., 3Marine Biosci., Kitasato Univ., 4Life, Environ. and Mat. Sci., Fukuoka Inst. Tech., 5Grad. Sch. Life and Environ. Sci., Kyoto pref. Univ.)
2Pos057 タンパク質ジスルフィドイソメラーゼ様触媒活性を有する新規低分子ジセレニド化合物を用いた酸化的フォールディングに関する研究Study on the oxidative folding by using a novel small-molecular diselenide compound having protein disulfide isomerase-like activity
Yuki Asano, Haruhito Ueno, Michio Iwaoka, Kenta Arai (Tokai Univ.)
2Pos058* 分子内ジスルフィド結合がリポカリン型プロスタグランジン D 合成酵素の熱安定性及び構造安定性に与える影響
Effects of an intramolecular disulfide bond on the thermal and conformational stability of lipocalin-type prostaglandin D synthase

2Pos059

2Pos060

Shogo Atsuji1, Yoshiaki Teraoka1, Young-Ho Lee2, Yuji Goto2, Takashi Inui1 (1Grad. Sch. Life & Envi. Sci., Osaka Pref. Univ., 2Inst. Prot. Res.,
Osaka Univ.)
タンパク質の翻訳時フォールディングにおけるリボソーム効果の粗視化分子動力学シミュレーション研究
Ribosomal effects on protein cotranslatoinal folding studied by coarse-grained molecular dynamics simulation
Kazushi Mochizuki, Shoji Takada (Graduate School of Science, Kyoto Univ.)
蛋白質の相互作用とダイナミクスに及ぼす高分子混み合いの影響：全原子分子動力学法による研究
Influence of Macromolecular Crowding on the Dynamics and Interactions of proteins: All-atom Molecular Dynamics Study
Isseki Yu1, Tadashi Ando2, Takaharu Mori1, Jaewoon Jung3, Ryuhei Harada3, Yuji Sugita1,2,3, Michael Feig4 (1RIKEN, 2RIKEN QBIC, 3RIKEN

AICS, 4Michigan State Univ.)
2Pos061* 高温条件下で変性した DEN4 ED3 の可逆的なオリゴマー形成の一残基置換による阻害
Unusual reversible oligomerization of unfolded Dengue envelope protein domain 3 at high temperature and its abolition by a point mutation
Tomonori Saotome1, Shigeyoshi Nakamura2, Mohammad M. Islam1, Akiko Nakazawa2, Mariano Dellarole3, Fumio Arisaka4, Shun-ichi Kidokoro2,
Yutaka Kuroda1 (1Dept. of Biotech. and Life Sci., Tokyo Univ. of Agric. and Tech., 2Dept. of Bioeng., Nagaoka Univ. of Tech., 3CBS, Univ. of
2Pos062

2Pos063

Montpellier, 4Coll. of Biores. Sci., Univ. of Nihon)
球殻状超分子集合における局所的相互作用の役割
The role of local interactions on the spherical shell-shaped supermoleculer assembly
Daisuke Sato1, Hideaki Ohtomo2, Atsushi Kurobe2, Kazuo Fujiwara1, Masamichi Ikeguchi1 (1Fac. of Sci. and Eng., Soka Univ., 2Fac. of Eng., Soka
Univ.)
赤外分光法によるカルシウム結合タンパク質の金属配位構造解析‐合成ペプチドアナログの凝集による問題
Infrared study of the Ca2+-coordination structures of Ca2+-binding proteins: the problem of aggregation of synthetic peptide analogues

Masayuki Nara1, Hisayuki Morii2, Takuya Miyakawa3, Masaru Tanokura3 (1TMDU, 2AIST, 3Grad. Sch. Agr. Life Sci., Univ. Tokyo)
2Pos064* 競争的凝集形成機構に基づいた蛋白質異常凝集の理解
Understanding of aberrant protein aggregation based on the competitive aggregation mechanism
Masayuki Adachi, Masatomo So, Yuji Goto (Inst. Protein Res., Osaka Univ.)
2Pos065 ヘパリンによるアミロイド線維形成の促進と抑制の分子機構
Molecular mechanism underlying the heparin-induced acceleration and inhibition of amyloid fibrillation
Ayame Nitani, Hiroya Muta, Masayuki Adachi, Masatomo So, Yuji Goto (Inst. Protein res., osaka Univ.)
2Pos066 キメラカルシトニンによるヒトカルシトニンアミロイド凝集阻害機構の解明
Analysis of amyloid formation and inhibition mechanisms of human calcitonin by chimera calcitonin
Chiaki Ota1, Hiroko Tanaka1, Tomoyasu Aizawa2, Yoichi Yamazaki1, Mikio Kataoka1, Hironari Kamikubo1 (1Grad. Sch. Mat. Sci., NAIST, 2Grad.
Sch. Life Sci., Hokkaido Univ.)
2Pos067* プリオンタンパク質とプリオンタンパク質を標的とする RNA 分子の Aβ 線維化への影響
The effects of prion protein and a RNA molecule that binds to prion protein on Aβ fibrillation
Mamiko Iida1,2, Tsukasa Mashima1,2, Yudai Yamaoki2, Takashi Nagata1,2, Masato Katahira1,2 (1Grad. Sch. of Energy Sci., Kyoto Univ., 2Inst. ofAdv. Energy, Kyoto Univ.)

蛋白質：機能 / Protein: Function
2Pos068

High-level expression, purification and characterization of the plant antimicrobial peptide snakin-1 in Pichia pastoris
Md. Ruhul Kuddus1,2, Farhana Rumi1, Motosuke Tsutsumi1, Megumi Yamano1, Masakatsu Kamiya1, Takashi Kikukawa1, Makoto Demura1,

Tomoyasu Aizawa1 (1Grad. Sch. Life Sci., Hokkaido Univ., 2Dhaka Univ.)
2Pos069 A study on tryptophan-dependent translation termination arrest of TnaC
Tomoki Shinozawa, Ryo Iizuka, Zhuohao Yang, Takashi Funatsu (Grad. Sch. of Pharm. Sci., The Univ. of Tokyo)
2Pos070* 1 分子不凍タンパク質の温度依存ダイナミクスと AgI との吸着関係
Temperature Dynamics of Single Molecular AntiFreeze Proteins and adsorption to AgI interaction
Rio Okada1, Tatsuya Arai2, Yuhuku Matushita1, Jae-won Chang1, Hiroshi Sekiguchi3, Tadashi Mori4, Masaki Nishijima4, Keigo Ikezaki1, Sakae

2Pos071

Tsuda2, Yuji Sasaki1,3 (1Frontier Science., Adv. Material Science., Tokyo Univ., 2AIST / Grad. Schl. of Life Sci., Hokkaido Univ., 3JASRI, 4Grad.
Schl. of Eng., Div. of Ap. Chem., Osaka Univ.)
青色光センサータンパク BlrP1 の反応の光強度依存性
Light intensity determines photoreaction of blue-light sensor protein BlrP1
Kosei Shibata, Yusuke Nakasone, Masahide Terazima (Grad. Sch. Sci. Unv. Kyoto)– S104 –2Pos072

2Pos073

反転膜小胞を用いたべん毛軸構造蛋白質の輸送順序の解析
Secretion order of the Class-II flagellar axial proteins analyzed by inverted membrane vesicles (IMV)Yudai Matsumoto1, Chinatsu Tatsumi1, Hiroyuki Terashima1, Tohru Minamino2, Katsumi Imada1 (1Grad. Sch. of Sci., Osaka Univ., 2Grad. Sch. of
Front. BioSci., Osaka Univ.)
Alp7/TACC-Alp14/TOG protein complex promotes assembly of S. pombe microtubules
Douglas Drummond1, Frauke Hussmann2, Daniel Peet2, Douglas Martin3, Robert Cross2 (1Faculty of Agriculture, Kyushu Univ., 2Warwick Medical

Sch., UK, 3Lawrence Univ., USA)タウタンパク質に対する Pin1 由来のプロテアーゼの活性Activity of a protease derived from Pin1 for tau protein
Teikichi Ikura, Nobutoshi Ito (Med. Res. Inst., Tokyo Med. Dent. Univ.)
2Pos075* アミロイド線維形成の圧力依存性を解明するための計算研究
Computational Research to Reveal the Pressure Dependency of the Formation of Amyloid Fibrils

2Pos074

Naohiro Nishikawa1,2, Yoshiharu Mori2, Yuko Okamoto1,3,4,5, Hisashi Okumura2,6 (1Grad. Sch. of Sci., Nagoya Univ., 2IMS, 3Str. Biol. Res. Cent.,
Grad. Sch. of Sci., Nagoya Univ., 4Cent. for Comp. Sci., Grad. Sch. of Eng., Nagoya Univ., 5Info. Tech. Cent., Nagoya Univ., 6SOKENDAI)

蛋白質：計測･解析 / Protein: Measurement & Analysis
2Pos076* ESI-QTOF MS 法を用いたアミロイドベータペプチドのオリゴマー形成機構の解析
ESI-QTOF MS analyses of the oligomerization mechanism of amyloid β peptides
Shintaro Yoshida1, Sosuke Yoshinaga1, Mitsuhiro Takeda1, Ayumi Tanaka1, Takashi Hamaguchi1, Hitomi Yamaguchi1, Shigeto Iwamoto1, Takashi
Saito2, Yoshihiko Takinami3, Toshiyuki Kohno4, Takaomi C. Saido2, Hiroaki Terasawa1 (1Fac. Life Sci., Kumamoto Univ., 2RIKEN BSI, 3Bruker
2Pos077

Daltonics, 4Kitasato Univ. Sch. Med.)
Processing of XFEL still images with a reference oscillation data set for crystal structural analyses of Cytochrome c Oxidase
Luo Fangjia1, Atsuhiro Shimada1, Keitaro Yamashita2, Kunio Hirata2, Masaki Yamamoto2, Kyoko Itoh-Shinzawa1, Shinya Yoshikawa1, Tomitake

2Pos078

Tsukihara1 (1Picobiology INST, Grad. Sch. of Life Sci., Univ. of Hyogo, 2RIKEN SPring-8 Center)
中性子タンパク質結晶構造解析での水素高感度検出のための動的核偏極法の予備的結果
The preliminary result of Dynamic Nuclear Polarization method for more sensitive detection of hydrogen in Neutron Protein
Crystallography
Naoya Komatsuzaki1, Ichiro Tanaka1,2, Takahiro Iwata3, Daisuke Miura3, Yoshiyuki Miyachi3, Genki Nukazuka3, Hiroki Matsuda3, Toshiyuki
Chatake4, Katsuhiro Kusaka2, Nobuo Niimura2 (1Coll. of Eng., Ibaraki Univ., 2Frontier Res. Ctr., Ibaraki Univ., 3Faculty of Sci., Yamagata Univ.,

2Pos079

2Pos080

2Pos081

4RRI, Kyoto Univ.)
Elucidating the mechanisms of proton pumping in cytochrome c oxidase by time resolved IR spectroscopy
Chen Li, Tatsuhito Nishiguchi, Shun Yamauchi, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Satoru Nakashima, Takashi Ogura (Grad. Sch. Sci.,
Univ. Hyogo)
ストップトフロー装置と過渡回折格子法を組み合わせたタンパク質反応検出手法の開発
Time-resolved detection of the transient grating signal using stopped flow system
Shunki Takaramoto, Yusuke Nakasone, Masahide Terazima (Dept. of chem, Univ. Kyoto)
顕微ラマン分光法による微小液滴内での酵素活性検出系の開発

Detection of enzymatic activity in femtliter droplets using micro-Raman spectroscopy
Hironobu Yamashita1, Kazuhito Tabata V.1,2, Hiroshi Ueno1, Hiroyuki Noji1,3 (1Department of Applied Chemistry, the University of Tokyo,
2PRESTO, JST, 3ImPACT, CAO, Govt)

2Pos082

2Pos083

2Pos084

蛋白質周囲の水分子運動の測定を目的とした蛍光アップコンバージョン測定装置の開発
Development of fluorescence up-conversion apparatus to investigate hydration dynamics around the protein surface
Asahi Fukuda, Tomotaka Oroguchi, Masayoshi Nakasako (Grad. Sci. Tech., Keio Univ.)
Grid inhomogeneous solvation theory を用いた血液凝固因子 Xa の水和解析
Hydration analysis of Coagulation Factor Xa using grid inhomogeneous solvation theory
Hiroyuki Sato, Azuma Matsuura (Fujitsu Laboratories Ltd.)キナーゼと ATP 競争阻害剤との結合自由エネルギー計算における、薬剤結合サイトの立体構造柔軟性がもたらす影響
The effect of conformational flexibility on the binding free energy calculation between kinases and their ATP-competitive inhibitors
Mitsugu Araki1, Narutoshi Kamiya1,2, Miwa Sato3, Masahiko Nakatsui1,5, Takatsugu Hirokawa4, Yasushi Okuno1,5 (1RIKEN, AICS, 2Grad. Sch.

Sim. St., Univ. Hyogo, 3Mitsui Knowledge Industry Co., Ltd., 4AIST, Molecular Profiling Research Center for Drug Discovery, 5Grad. Sch. Med.,
Kyoto Univ.)
2Pos085* 溶質構造エントロピー計算法の理論的研究
Theoretical study for solute configurational entropy calculation methods
2Pos086

Simon Hikiri1, Takashi Yoshidome1,2, Mitsunori Ikeguchi1 (1Grad. Sch. of Med. Life Sci. Yokohama city Univ., 2Dept. of Appl. Phys., Tohoku Univ.)
分子シミュレーションにおける静電相互作用計算法：零多重極子法の理論と実際
Computational method for electrostatic interactions in molecular simulation: theory and practice in the zero-multipole summation
Ikuo Fukuda1, Han Wang2, Narutoshi Kamiya3, Kota Kasahara4, Tohru Terada5, Shun Sakuraba6, Haruki Nakamura1 (1IPR, Osaka Univ., 2Freie
Universitaet Berlin, 3Univ. of Hyogo, 4Col. Life Sci., Ritsumeikan Univ., 5Grad. Sch. Agr. Life Sci. Univ. Tokyo, 6Grad. Sch. Frontier Sci., Univ.
Tokyo)– S105 –蛋白質工学 / Protein: Engineering
2Pos087

2Pos088

天然状態と似たそして異なるアポミオグロビン折り畳み中間体の構造
Native-like and non-native structures in the folding intermediate of apomyoglobin
Chiaki Nishimura (Fac. Pharm. Sci., Teikyo Heisei Univ.)
理想タンパク質の安定性のオリジンを探る
Stability for de novo designed ideal proteins revisited

2Pos089

Mami Yamamoto1,2, Rie Koga1, Takahiro Kosugi1,2, Nobuyasu Koga1,2,3 (1CIMoS, IMS., 2SOKENDAI, 3JST, PRESTO)
c-Myb- KIX 間相互作用を阻害するペプチドの合理的設計
Rational design of a peptide inhibitor of the c-Myb-KIX interaction

2Pos090

Shunji Suetaka1, Yoshiki Oka2, Yuuki Hayashi1,2, Munehito Arai1,2 (1Dept. Integrated Sci., Univ. Tokyo, 2Dept. Life Sci., Univ. Tokyo)
β シートモデルタンパク質 OspA への αB クリスタリンのアミロイド形成配列移植と評価
Grafting of a short amyloid forming sequence from αB crystalline into β-rich model protein, OspA
Yuki Hori1, Kenta Hongo2, Norio Yoshida3, Koki Makabe1 (1Graduate School of Science and Engineering, Yamagata University, 2School ofInformation Science, JAIST, 3Department of Chemistry, Graduate School of Sciences, Kyushu University)
2Pos091 レアメタルに特異的に結合するペプチドの設計
Design of peptides that specifically bind to the rare metal
Yoshihiro Iida, Atsuo Tamura (Grad. Sci., Univ. Kobe)
2Pos092* 進化分子工学によるフィチン酸塩加水分解酵素の活性向上
Improving activity of a phytate-hydrolyzing enzyme by directed evolution
Manami Wada, Yuuki Hayashi, Munehito Arai (Dept. Life Sci., Univ. Tokyo)
2Pos093

Toward Directed Evolution of Bacterial Biosensor for Arsenite Detection by Compartmentalized Partnered Replication
Seaim Lwin Aye, Asuka Ueki, Kei Fujiwara, Nobuhide Doi (Grad. Sch. Sci. Tech., Keio Univ.)
2Pos094 フェムトリットルチャンバーアレイを用いたスクリーニングシステムによるアルカリフォスファターゼの進化分子工学
Directed evolution of alkaline phosphatase by femtoliter chamber array screening system
Makoto Kato, Yi Zhang, Kazuhito Tabata, Hiroyuki Noji (Grad. Sch. Eng., Univ. Tokyo)
2Pos095* ファージ選別実験への応用を目指したスプリット GFP による蛍光ファージの作製
Construction of fluorescent phages based on split GFP for the phage sorting technique
Naoki Mikoshiba1,2, Yuki Shimizu1,3, Rie Kiriguchi1,2, Seiji Sakamoto1,3, Hiroyuki Oikawa1,2,3, Kiyoto Kamagata1,2,3, Takehiko Wada1,3, Satoshi
2Pos096
2Pos097

Takahashi1,2,3 (1IMRAM, Tohoku Univ., 2Grad. Sch. Life Sci., Tohoku Univ., 3Grad. Sch. Sci., Tohoku Univ.)
Toward design of diverse all-α protein structures
Kouya Sakuma1,2, Rie Koga1, Takahiro Kosugi1,2, Nobuyasu Koga1,2,3 (1CIMoS, IMS., 2SOKENDAI, 3JST, PRESTO)
エクソンペプチドの構造予測と解析
Prediction of Exon-Peptide Structures and Analysis
Michiko Nosaka (National Institute of Technology, Sasebo College)

核酸結合蛋白質 / Nucleic acid binding proteins
2Pos098* 粗視化分子動力学計算によるサブヌクレオソームの構造解析
Structural modeling of the subnucleosome using coarse-grained molecular dynamics simulations
Masahiro Shimizu, Shoji Takada (Grad. Sch. Sci., Kyoto Univ.)
2Pos099 Holliday junction DNA facilitates RuvA-RuvB complex formation
Yong-Woon Han1, Reiko Yamamoto1, Kimiko Nakao1, Hisashi Tadakuma1, Yoshie Harada1,2,3 (1Kyoto Univ., iCeMS, 2Grad. Sch. Biostudies,2Pos100

Kyoto Univ., 3Inst. for Protein Res., Osaka Univ.)
定量的イメージング法を用いた単一細胞由来のグルココルチコイド受容体のホモ二量体と転写活性の関連解析
Quantification of homodimeric glucocorticoid receptor and transcriptional activity from single cell using quantitative imaging techniques

2Pos101

Sho Oasa1, Akira Sasaki2, Shintaro Mikuni1, Johtaro Yamamoto1, Masataka Kinjo1 (1Fac. Adv. Life Sci., Hokkaido Univ., 2AIST)(6-4)光回復酵素の T(6-4)T と T(6-4)C の光活性及び光修復における 赤外分光解析の比較
Comparative FTIR study of photoactivation and photorepair of T(6-4)T and T(6-4)C photoproducts by Xenopus (6-4) photolyase
Mai Kumagai1, Daichi Yamada1,2, Tatsuya Iwata1, Elizabeth D Getzoff4, Junpei Yamamoto3, Shigenori Iwai3, Hideki Kandori1 (1Nagoya Institute2Pos102of Technology, 2Ochanomizu University, 3Osaka University, 4The Scripps Research Institute)
3 本鎖 DNA 結合蛋白質が 3 本鎖 DNA を認識する分子機構
Molecular mechanism of the triplex DNA-binding protein to recognize triplex DNA
Kazuki Kiuchi1, Kohta Sugiyama1, Ryotaro Kishi1, Satoru Unzai2,3, Hidetaka Torigoe1 (1Dept. Applied Chem., Fac. Sci., Tokyo Univ. Sci., 2Grad.

Sch. Medical Life Sci., Yokohama City Univ., 3Fac. Biosci. Applied Chem., Hosei Univ.)
2Pos103* Characterization of the deamination activity of APOBEC3B by real-time NMR, which is distinct from that of APOBEC3G
Li Wan1, Takashi Nagata1,2, Ryo Morishita3, Akifumi Takaori4, Masato Katahira1,2 (1Grad. Sch. of Energy Sci., Kyoto Univ., 2Inst. of Adv. Energy,
2Pos104

Kyoto Univ., 3CellFree Sciences Co., Ltd., 4Dept. of Hematology and Oncology, Grad. Sch. of Med., Kyoto Uni.)
Sequence-Specific Protein-DNA Interactions for Molecular Simulations Modeled by Position Weight Matrix
Cheng Tan, Shoji Takada (Dept. Biophysics, Graduate School of Science, Kyoto University)– S106 –2Pos105

Mechanistic studies of transcriptional regulation by non-CpG methylated DNA
Jianshi Jin1,2,4, Tengfei Lian1,2, Chan Gu1,3, Kai Yu1,2, Yi Qin Gao1,3, Xiao-Dong Su1,2 (1Biodynamic Optical Imaging Center(BIOPIC)，Peking

2Pos106

2Pos107

University, 2School of Life Sciences, Peking University, 3College of Chemistry and Molecular Engineering, Peking University, 4RIKEN Quantitative
Biology Center (QBiC), Laboratory for Integrative Omics)
転写調節機構の統計的解釈〜コンセンサス配列でわかること、わからないこと〜
Global Statistical Control of Transcriptional Pausing by Repetitive Genomic Sequences
Masahiko Imashimizu1,2,3, Ariel Afek4, Hiroki Takahashi2, David Lukatsky4 (1IMSUT, 2MMRC, Chiba Univ., 3NIH/NCI, 4Ben-Gurion Univ. of the
Negev)
Molecular dynamics of transcription factor-nucleosome interactions
Giovanni Brandani, Shoji Takada (Kyoto University)

核酸：構造・物性 / Nucleic acid: Structure & Property
2Pos108

Structured RNAs Induce Intersubunit Rolling and Codon-Anticodon Weakening During Ribosomal Frameshifting
Kaichun Chang1, Emmanuel Salawu2,3, Jin-Der Wen1, Lee-Wei Yang2,3,4 (1National Taiwan University, 2National Tsing Hua University,
3Bioinformatics Program, Academia Sinica, 4National Center of Theoretical Science)

2Pos109

リンカー DNA により繋がっているダイヌクレオソーム構造のサンプリング
Sampling di-nucleosome structures connected by linker DNA of various lengths

2Pos110

Hiroo Kenzaki1, Shoji Takada2 (1ACCC, RIKEN, 2Grad. Sch. Sci., Univ. Kyoto)
酵母間期染色体の力学モデルと核内構造・動態の解析
Analysis of interface chromosome dynamics of yeast by course-grained models

2Pos111

Takamasa Yamamoto1, Hiraku Nishimori1,2, Akinori Awazu1,2 (1Dept. Math and Life Sci. Hiroshima Univ., 2RcMcD)
細胞分裂およびアポトーシス過程におけるクロマチンダイナミクスの 1 分子解析
Chromatin dynamics in mitosis and apoptosis

2Pos1122Pos113

Kayo Hibino1,2, Kazuhiro Maeshima1,2 (1NIG, 2SOKENDAI)
Grab & Watch: Correlative optical Tweezers-Fluorescence Microscopy (CTFM) as a versatile tool for chromatin studies
Andrea Candelli1,2, Gerrit Sitters1,2, Rosalie Driessen1,2, Willem Peutz1,2, Olivier Heyning1,2, Gijs Wuite1,2, Erwin Peterman1,2 (1LUMICKS, 2VU
University, Amsterdam)
クロマチンのエピジェネティック状態を用いたヒト間期核内における染色体三次元構造のシミュレーション
Simulating three-dimensional organization of chromosomes in human interphase nucleus using epigenetic state of chromatin
Shin Fujishiro, Masaki Sasai (Nagoya Univ.)

核酸：相互作用･複合体形成 / Nucleic acid: Interaction & Complex formation
2Pos114* 蛍光相互相関分光法を用いたグルココルチコイド受容体-DNA 間相互作用の定量化
The different interaction affinity of monomeric or dimeric GR on DNA determined by fluorescence cross-correlation spectroscopy
Daisuke Yamashita, Mari Saito, Sho Oasa, Shintaro Mikuni, Masataka Kinjo (Grad. Sch. Life Sci., Univ. Hokkaido)
2Pos115 Effects of cumulative acetylation in histone H3 tail studied by an enhanced conformational sampling MD simulation
2Pos116
2Pos117

2Pos118

2Pos119

Jinzen Ikebe1, Shun Sakuraba2, Hidetoshi Kono1 (1QST, MMS, 2Grad. School of Frontier Sci., Univ. Tokyo)
Photo-control of the ribosome movement along mRNA using a reversible photo-cross-linking probe
Shunsuke Yamashiro1, Ryo Iizuka2, Takashi Funatsu2 (1Fac. of Pharm. Sci., The Univ. of Tokyo, 2Grad. Sch. of Pharm. Sci., The Univ. of Tokyo)
転写によって誘起されるクロマチンブラシの相分離
Transcription driven phase separation in chromatin brush
Tetsuya Yamamoto1, Helmut Schiessel2 (1National Composite Center, Nagoya University, 2Instituut-Lorentz for Theoretical Physics, Leiden
University)
蛍光消光現象を利用した単層カーボンナノチューブ表面上での一本鎖 DNA 吸着過程の観察
Monitoring adsorption process of single stranded DNA to single-walled carbon nanotubes surfaces by fluorescence quenching
Shizuma Sato1, Gilbert Bustamante2, Jing Yong Ye2, Kazuo Umemura1 (1Tokyo Univ. of Sci., 2UTSA)DNA の網目構造が微粒子の拡散に及ぼす影響
Effect of a mesh structure of DNA on diffusion of a small particle
Masaya Tanoguchi, Yoshihiro Murayama (Tokyo Univ. of Agri. and Tech.)

水･水和･電解質 / Water & Hydration & Electrolyte
2Pos120

2Pos121

イオン液体ー水混合溶液中の β-ラクトグロブリンの会合構造
Aggregation of β-lactoglobulin in alkylammonium-based nitrate ionic liquid-water mixture
Koji Yoshida, Ayako Fujiyoshi, Toshio Yamaguchi (Fukuoka Univ.)
OH 伸縮振動のラマン分光によるアミノ酸水和層の解析
Hydration analysis of amino acids by Raman OH-stretching spectroscopy
Yasutaka Naito1, Yuki Ochiai2, George Mogami2, Makoto Suzuki2 (1Sch. Eng., Univ. Tohoku, 2Grad. Sch. Eng., Univ. Tohoku)– S107 –2Pos122

広帯域誘電緩和分光を用いた球状および膜タンパク質における水和と熱活性効果の研究
Effects of hydration and thermal excitation of globular and membrane proteins studied by broadband dielectric spectroscopy
Naoki Yamamoto1, Shota Ito2, Eri Chatani1, Hideki Kandori2, Keisuke Tominaga1,3 (1Graduate School of Science, Kobe University, 2Graduate

School of Engineering, Nagoya Institute of Technology, 3Molecular Photoscience Research Center, Kobe University)
2Pos123* 分子動力学法を用いた蛋白質周囲の水和ダイナミクスの検討：溶媒条件と蛋白質構造の影響について
Effects of solvent pH and protein conformations on water dynamics around a denatured protein with molecular dynamics simulation
Takafumi Fujiyoshi1, Naoki Ogasawara1, Yuji Ezaki2, Yuta Nonaka2, Kota Kasahara2, Takuya Takahashi2 (1Graduate School of Life Sciences,
Ritsumeikan University, 2College of Life Sciences, Ritsumeikan University)
2Pos124 ミオシン周囲の局所誘電環境
Local dielectric environment around myosin
Takato Sato, Tohru Sasaki, Jun Ohnuki, Mitsunori Takano (Dept. of Pure. & Appl. Phys., Waseda Univ.)
2Pos125 水溶液中における芳香環間相互作用の統計熱力学
Statistical thermodynamics of aromatic-aromatic interactions in aqueous solution
Tomohiko Hayashi, Masahiro Kinoshita (Inst. Adv. Energy, Kyoto Univ.)
2Pos126* Lennard-Jones ポテンシャルのパラメータの変更による溶質周囲の水分子のダイナミクスの探求
Effects of Lennard-Jones potentials on the dynamics of water molecules around a solute2Pos127

2Pos128

Yuki Takimoto1, Kou Sakuma2, Nana Okita2, Kota Kasahara2, Takuya Takahashi2 (1Grad. Sch. Life. Sci., Ritsumeikan Univ., 2Col. Life. Sci.,
Ritsumeikan Univ.)
親水性アミノ酸残基周辺の水和構造における酸素原子ローンペアの影響
Effects of lone pairs of oxygen atoms on hydration structures around polar amino-acid residues: MD simulation study
Tomotaka Oroguchi, Masayoshi Nakasako (Sci. Tech., Keio Univ.)
機械学習によるタンパク質親水／疎水表面における水分子の動的振る舞いの解析
Analysis of water behavior near the protein hydrophobic/hydrophilic surface by machine learning techniques

Taku Mizukami1, Nguyen Viet Cuong3, Ho Tu Bao2, Dam Hieu Chi2 (1JAIST, Materials Science, 2JAIST, Knowledge Science, 3HPC SYSTEMS
Inc.)
2Pos129* MM/3D-RISM 法を用いた Pim1-リガンド系における結合自由エネルギーの予測
Estimation of binding free energy based on the MM/3D-RISM method for the Pim1-ligand system
Takeshi Hasegawa1, Masatake Sugita1, Takeshi Kikuchi1, Fumio Hiata2 (1Dept. of Bioinfo., Col. Life Sci., Ritsumeikan Univ., 2Toyota Physical andChemical Research Institute)

発生･分化 / Development & Differentiation
ROXS によって mCherry の安定性と明るさが向上する
in-vivo ROXS improve the brightness and photostability of a red fluorescent protein, mCherry in C. elegans embryos
Yukinobu Arata, Yasushi Sako (Cell. Info. Lab. RIKEN)
2Pos131 Axon bundle regulates cortical tissue stiffness in the developing brain
Misato Iwashita, Yoichi Kosodo (Korea Brain Research Institute)
2Pos132* Mouse-ferret differences in the mechanical property of the developing cerebral cortex: tissue-level and single cell-level assessments
Arata Nagasaka, Tomoyasu Shinoda, Takaki Miyata (Grad. Sch. Med., Univ. Nagoya)
2Pos133 Macroscopic dynamics of vascular endothelial cells in angiogenesis

2Pos130

Naoko Takubo1, Kazuaki Naekura1, Ryo Yoshida2, Terumasa Tokunaga3, Osamu Hirose4, Yasunobu Uchijima1, Yukiko Kurihara1, Hiroki
Kurihara1 (1Graduate School of Medicine, The University of Tokyo, 2The Institute of Statistical Mathematics, Research Organization of Information

2Pos134

and Systems, 3Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, 4College of Science and Engineering,
Kanazawa University)
ヒトの原腸形成時の細胞運動を in vitro で一細胞解析
Single cell tracking of migration during human gastrulation in vitro
Shota Miyazaki1, Yuta Yamamoto1, Kohei Nakazono1, Minh N. T. Le1, Shuji Fuji1,2, Kiyoshi Ohnuma1,3 (1Department of Bioengineering, Nagaoka

2Pos135

University of Technology, 2Department of Materials Science and Technology, Nagaoka University of Technology, 3Department of Science ofTechnology Innovation, Nagaoka University of Technology)
多細胞運動におけるソリトン様運動関連遺伝子の探索
Searching the responsible genes for biological soliton in multicellular movement
Kentaro Yoshida1, Hiroki Takahashi2, Yoshitoshi Ogura3, Yutaka Suzuki4, Tetsuya Hayashi3, Hidekazu Kuwayama1 (1Fac. Life and Env. Sci.,

2Pos136
2Pos137

Univ. Tsukuba, 2Medi. Myco. Res. Center, Chiba Univ., 3Dep. Bact., Fac. Med. Sci., Kyushu Univ., 4Dep. Med. Genome Sci., Grad. School Front.
Sci., Univ. Tokyo)
A model for analyzing phenomena in multicellular organisms with multivariable polynomials
Hiroshi Yoshida (Dep. Math., Kyushu Univ.)
C. elegans の受精における二相性カルシウム波の分子基盤
The molecular underpinnings of the biphasic calcium wave during fertilization in C. elegans
Jun Takayama, Shuichi Onami (RIKEN QBiC)– S108 –筋肉 / Muscle
2Pos138

2Pos139

2Pos140機能する昆虫飛翔筋-ウサギ骨格筋ハイブリッド筋線維の再構成
Reconstitution of functional insect flight- and rabbit skeletal hybrid muscle fibers as monitored by X-ray diffraction
Hiroyuki Iwamoto (SPring-8, JASRI)
アクチン骨格阻害剤 Latrunculin A はアクチンを脱重合させ重合を阻害する
Latrunculin-A; a drug to inhibit actin cytoskeleton, depolymerizes and inhibits actin polymerization under TIRF microscopy
Ikuko Fujiwara1, Thomas Pollard2 (1URA, NITech., 2MCDB, Yale Univ.)
バキュロウイルス-昆虫細胞を用いた、組換え β-アクチンの発現精製系の構築
Expression and Purification of Recombinant Human β-Actin in Insect Cells
Mizuki Matsuzaki1, Sae Kashima1, Kayo Maeda1, Mahito Kikumoto1, Tomoharu Matsumoto1, Kotaro Tanaka1, Motonori Ota2, Akihiro Narita1(1Grad. Sch. Sci., Nagoya Univ., 2Grad. Sch. Info. Sci., Nagoya Univ.)
2Pos141 結合した HMM 濃度の違いによる F-アクチンの状態変化
HMM induced structural changes of actin monitored by in vitro fluorescence along single filaments
Marie Kobayashi, Satoshi Nakamura, Takahiro Hayashizaki, Hajime Honda (Dept. Bioeng., Nagaoka Univ. Tech.)2Pos142* 超解像イメージング法を用いた骨格筋ミオシン分子動態の直接計測
Direct measurement of dynamics of individual skeletal myosin by using a super-resolution localization method
ZhiYuan Zhang, Yuto Ashida, Masahito Ueda, Hideo Higuchi, Motoshi Kaya (Department of Physics, Graduate School of Science, The Universityof Tokyo)

分子モーター / Molecular motor
2Pos143* HS-AFM で一方向的な運動が観測されたローターレス Enterococus hirae V1-ATPase の結晶構造解析
Crystal structures of rotorless Enterococus hirae V1-ATPase which shows unidirectional dynamics by HS-AFM
Shintaro Maruyama1, Kazuya Nakamoto1, Kano Suzuki1, Fabiana. Lica Yakushiji1, Kenji Mizutani1, Motonori Imamura2, Takayuki Uchihashi2,3,4,
2Pos144

Takeshi Murata1,5 (1Grad. Sch. Sci., Univ. Chiba, 2Bio-AFM FRC, Kanazawa Univ., 3Dept. Phys., Kanazawa Univ., 4CREST, JST, 5PRESTO, JST)
F1-ATPase の中心軸回転を駆動する触媒サブユニットの１分子構造変化観察
Single-molecule detection of conformational changes in the catalytic subunit of F1-ATPase that correlate with rotation of the shaft
Ryuichi Yokota1, Ryota Yanagida1, Yuta Nomura1, Nagisa Mikami2, Rinako Nakayama2,3, Takayuki Nishizaka2, Tomoko Masaike1,3,4 (1Dept.

Appl. Biol. Sci., Tokyo Univ. of Sci., 2Dept. Phys., Gakushuin Univ., 3Res. Inst. for Sci. and Tech., Tokyo Univ. of Sci., 4PRESTO, JST)
2Pos145* V1 回転分子モーターでの外来タンパク質の回転
Rotation of endogenous proteins in V1 rotary motor
Mihori Baba, Atsuko Nakanishi, Jun-ichi Kishikawa, Ken Yokoyama (Kyoto Sangyo Univ. LifeSci.)
2Pos146* 1 分子観察とドッキングシミュレーションで蛍光基質を通じて明らかになった化学反応に伴う酵素の構造変化
Conformational change of the rotary motor F1-ATPase revealed by single-molecule imaging and docking simulation

2Pos147

Nagisa Mikami1, Yuko Ito2, Mitsuhiro Sugawa1, Mitsunori Ikeguchi2, Takayuki Nishizaka1 (1Dept. phys., Gakushuin Univ., 2Medical Life Sci.,
Yokohama City Univ.)
光渦トラップを使った力学測定システムの開発：DNA オリガミを使った F1-ATPase の回転可視化
Development of optical vortex trapping system with DNA origami for the precise measurements of torque generated by F1-ATPase
Yu Hashimoto1, Sayaka Kazami1, Yuji Kimura1, Tomoko Hyodo-Otsu2, Taro Ando2, Hiroyasu Itoh1,2 (1Tsukuba Research Lab., Hamamatsu

2Pos148

Photonics K.K., 2Central Research Lab., Hamamatsu Photonics K.K.)
Enterococcus hirae 由来 V1-ATPase の回転とヌクレオチド結合解離の１分子同時観察
Single-molecule simultaneous observation of rotation and nucleotide binding/release of Enterococcus hirae V1-ATPase
Yoshihiro Minagawa1, Hiroshi Ueno1, Hiroyuki Noji1, Takeshi Murata2, Ryota Iino3,4 (1Univ. Tokyo, 2Chiba Univ., 3OIIB and IMS, NINS,
4SOKENDAI)

2Pos149

高熱菌 Bacillus PS3 由来 FoF1-ATP 合成酵素のプロトン輸送活性および H+/ATP の決定
Determination of the proton pump activity and the H+/ATP ratio of thermophilic Bacillus PS3 FoF1-ATP synthase
Naoya Iida1, Yuzo Kasuya1, Naoki Soga2, Toshiharu Suzuki2, Taro Uyeda1, Masasuke Yoshida3, Kazuhiko Kinosita1 (1Dept. Physics, Waseda

2Pos150

2Pos151

Univ., 2Dept. Eng, Univ. of Tokyo, 3Dept. Mol Biochem, Kyoto Sangyo Univ.)
細菌べん毛モーターの回転方向変換制御機構の解明
Elucidation of the directional switching mechanism of the bacterial flagellar motor by electron cryomicroscopy
Tomoko Miyata1, Takayuki Kato1, Akihiro Kawamoto1, Keiichi Namba1,2 (1Grad. Sch. Frontier Biosci., Osaka Univ., 2QBiC, RIKEN)
Molecular dynamics study of pressure effects on unbinding of the CheY-FliM complex
Hiroaki Hata1, Yasutaka Nishihara1, Masayoshi Nishiyama2, Ikuro Kawagishi3, Akio Kitao1 (1IMCB, UTokyo, 2The HAKUBI Center, Kyoto Univ.,
3Dept. of Frontier Biosci., Hosei Univ.)

2Pos152

海洋性ビブリオ菌 FliG の EHPQR-motif 周辺構造によるべん毛の回転方向決定
Direction of flagellar motor rotation determined by the structure around EHPQR-motif of FliG in marine Vibrio
Tatsuro Nishikino1, Atsushi Hijikata2, Yasuhiro Onoue1, Tsuyoshi Shirai2, Michio Homma1 (1Div. Biol. Sci. Grad. Sch. Sci., Nagoya Univ., 2Dep.
Biosci., Nagahama Inst. of Bio-Sci. Tec.)– S109 –2Pos153* 2 種イオン駆動型べん毛モーターの入力と出力の関係
Input-output relationship of dual ion driven flagellar motor
Kenta Arai1, Taishi Kasai2, Yuka Takahashi3, Masahiro Ito3, Yoshiyuki Sowa1,2 (1Hosei Univ., 2Research Center for Micro-Nano Tech. Hosei
2Pos154

2Pos155

2Pos156

Univ., 3Toyo Univ.)
高度高塩菌ハロバクテリウムサリナラムのべん毛の回転とステップ運動の直接観察
Direct observation of rotation and steps of the archaellum in the swimming halophilic archaeon Halobacterium salinarum
Yoshiaki Kinosita1, Nariya Uchida2, Daisuke Nakane1, Takayuki Nishizaka1 (1Department of Physics, Gakushuin University, 2Department of
Physics, Tohoku University)
キネシンネックリンカーの構造変化に伴う自由エネルギー変化
Measuring of energy at neck linker docking of single kinesin molecule
Yuichi Kondo, Hideo Higuchi (Grad. Sch. Sci., Univ. of Tokyo)
LZMW を利用した高濃度蛍光 ATP 存在下でのキネシン運動と ATP 結合の同時蛍光 1 分子計測
Simultaneous fluorescent observation of kinesin motility and ATP occupancy with high concentration of fluorescent labeled ATP using
LZMW
Kazuya Fujimoto1, Yuki Morita1, Hirofumi Shintaku1, Michio Tomishige2, Ryota Iino3, Hidetoshi Kotera1, Ryuji Yokokawa1 (1Kyoto University

2Pos157

Department of Micro Engineering, 2The University of Tokyo Department of Applied Physics, 3National Institute of Natural Science Institute for
Molecular Science)
CYK-4 による kinesin-6 の回転運動揺らぎ
CYK-4 induces the large fluctuations of the left-handed rotational movement of dimeric kinesin-6
Yohei Maruyama1, Akihiko Sato1, Tim Davis2, Toshihisa Osaki3, Shin Yamaguchi1, Shoji Takeuchi3, Masanori Mishima2, Junichiro Yajima1

2Pos158(1Dept. Life Sci., Grad. Sch. of Arts and Sci., Univ. of Tokyo, 2CMCB at Warwick Med. Sch., Univ. of Warwick, 3Inst. of Ind. Sci., Univ. of Tokyo)
微小管と光応答性 DNA による物質輸送システムの構築
Construction of a nano-transportation system by using microtubules and photoresponsive DNA
Kentaro Kayano1, Ryuuhei Suzuki2, A.M.R. Kabir2, Kazuki Sada2,3, Akinori Kuzuya4, Hiroyuki Asanuma5, Akira Kakugo2,3 (1Dept. Chem. Fac.

2Pos159

Sci., Hokkaido Univ., 2Grad. Sch. Chem. Sci. Eng., Hokkaido Univ., 3Fac. Sci., Hokkaido Univ., 4Fac. Chem. Mater. Bioeng., Kansai Univ., 5Grad.
Sch. Eng., Nagoya Univ.)
キネシンの非平衡熱散逸
Non-equilibrium dissipation of kinesin

Takayuki Ariga1, Michio Tomishige2, Daisuke Mizuno1 (1Dept. of Phys., Kyushu Univ., 2Dept. of Appl. phys., Univ. Tokyo)
2Pos160 生細胞内における微小管へのキネシン結合速度定数の直接計測
Direct measurement of the binding rate constant of kinesin to microtubules in living cells
Taketoshi Kambara, Yasushi Okada (RIKEN, QBiC)
2Pos161 Flavobacterium johnsoniae の滑走に関与するマルチレール構造
The multi-rail structure contributes to gliding motility of Flavobacterium johnsoniae
Satoshi Shibata, Koji Nakayama (Graduate Sch. of Biomedical Sciencces, Nagasaki Univ.)
2Pos162* マイコプラズマ・モービレ滑走運動におけるシングルユニットが発生する力
Force generated by single unit in Mycoplasma mobile gliding

2Pos163
2Pos164

Masaki Mizutani1, Isil Tulum1, Yoshiaki Kinosita2, Takayuki Nishizaka2, Makoto Miyata1 (1Osaka City Univ., Grad. sch. Sci., 2Gakushuin Univ.,
Fac. Sci.)
Dynamics of Type IV pili controlled by light direction in unicellular cyanobacteria
Daisuke Nakane, Takayuki Nishizaka (Dept. of Phys., Gakushuin Univ.)
マイコプラズマモービレ由来滑走タンパク質 Gli349 の構造ドメインの探索とその構造解析
Determination of domain boundaries and analysis of domain structures of the gliding protein Gli349 from Mycoplasma mobile
Yuuki Hayashi1,2, Yoshihiro Nomura2, Manami Wada1, Tasuku Hamaguchi3, Aya Takamori3, Masato Miyata3, Munehito Arai1,2 (1Dept. Life Sci.,

2Pos165

Univ. Tokyo, 2Dept. Integrated Sci., Univ. Tokyo, 3Dept. Biol., Osaka City Univ.)
高圧力顕微鏡法による深海微生物の遊泳運動観察
Swimming motility of deep-sea bacteria measured by high-pressure microscopy
Masayoshi Nishiyama1, Chiaki Kato2, Hiroshi Imai3, Shinji Kamimura3, Yoshie Harada4 (1The HAKUBI Center, Kyoto Univ., 2JAMSTEC, 3Chuo

Univ., 4Osaka Univ.)
2Pos166* Mycoplasma mobile の滑走装置に局在するペアになった F 型 ATP アーゼのパラログ
Paired F-type ATPase paralog in gliding machinery of Mycoplasma mobile
Takuma Toyonaga1, Yuhei Tahara1, Noriyuki Kodera2, Tasuku Hamaguchi1, Toshio Ando2, Makoto Miyata1 (1Grad. Sch. Sci., Osaka City Univ.,
2Bio AFM-FRC., Kanazawa Univ.)

2Pos167* Dynamics and heterogeneity of ATP production and consumption in single C2C12 myotubes
Naoki Matsuda1, Katsuyuki Kunida1, Takumi Wada1, Haruki Inoue2, Daisuke Hoshino1, Shinya Kuroda1,2 (1Grad. Sch. Sci., Univ. Tokyo, 2Grad.
Sch. Frontier Sci., Univ. Tokyo)– S110 –細胞生物学 / Cell biology
2Pos168

C 型インフルエンザウイルスの運動機構
Motile mechanism of influenza C virus
Tatsuya Sakai1, Yasushi Muraki2, Mineki Saito1 (1Department of Microbiology, Kawasaki Medical School, 2Division of Infectious Diseases and2Pos169

Immunology, Department of Microbiology, School of Medicine, Iwate Medical University)
鞭毛・繊毛の表面運動：現象の普遍性と膜タンパク質のダイナミクス
Surface motility in eukaryote cilia/flagella: Generality and membrane protein dynamics
Ritsu Kamiya1,3, Kogiku Shiba2, Kazuo Inaba2, Takako Kato-Minoura3 (1Gakushuin Univ., Fac. Sci., 2Tsukuba Univ., Shimoda Marine Res. Ctr.,
3Chuo Univ., Fac. Sci. Eng.)

2Pos170

微小管ネットワークによって引き起こされる細胞質回転流動
Spatial confinement of active microtubule networks induces large-scale rotational cytoplasmic flow
Kazuya Suzuki1,2, Makito Miyazaki1,2, Jun Takagi3, Takeshi Itabashi1,2, Shin’ichi Ishiwata1 (1Dept. Physics, Waseda Univ., 2Waseda Bioscience2Pos171

2Pos172

2Pos173

Research Institute in Singapor, Waseda Univ., 3Quantitative Mechanobiology Lab., NIG)
軸糸直径サイズ変化による鞭毛繊毛の屈曲運動の制御
Regulation of cilia and flagella bending movements through the change of axoneme diameter
Toshiki Yagi1, Shinji Kamimura2, Hiroyuki Iwamoto3 (1Dept. Life Sci., Prefectural Univ. of Hiroshima, 2Dept. Biol. Sci., Chuo Univ., 3SPring-8
JASRI)
中心子の普遍的 9 回対称性構造の構築機構
Assembly mechanisms of the nine-fold symmetry of the centriole structure
Masafumi Hirono (Frontier Biosci. Hosei Univ.)
X 線繊維回折を用いた真核生物鞭毛軸糸の構造ダイナミクス解析
X-ray fiber diffraction study on structural dynamics of flagellar axonemes of Chlamydomonas

Kazuhiro Oiwa1,2, Hiroyuki Iwamoto3, Junya Kirima2, Yu Yamano2 (1Adv. ICT Res. Inst. NICT, 2Univ. Hyogo, 3SPring8, JASRI)
2Pos174* 3-D measurement of bending dependency of the maximum force of the single tracheal cilium
Takanobu A Katoh1, Koji Ikegami2, Toshihito Iwase3, Tomoko Masaike3,4, Mitsutoshi Setou2, Takayuki Nishizaka1 (1Dept. Phys., Gakushuin
2Pos175

Univ., 2Dept. Cell Biol. and Anat., Hamamatsu Univ. Sch. Med., 3Dept. Appl. Biol. Sci., Tokyo Univ. of Sci., 4PRESTO, JST)
アルファシヌクレインタンパク質による輸送性微小管の制御機構
Alpha-synuclein binds unconventional microtubules that have a unique function
Shiori Toba1, Mingyue Jin1, Masami Yamada1, Takuo Yasunaga2, Yuko Fukunaga3,4, Atsuo Miyazawa3,4, Kyoko Itoh5, Shinji Fushiki5, Hiroaki
Kojima6, Hideki Wanibuchi7, Yoshiyuki Arai8, Takeharu Nagai8, Shinji Hirotsune1 (1Dept. of Genetic Disease Research, Osaka City Univ. Graduate
School of Medicine, 2Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, 3Graduate School of Life Science,
Univ. of Hyogo, 4RSC-University of Hyogo Leading Program Center, RIKEN SPring-8 Center, 5Kyoto Prefectural Univ. of Medicine Graduate
School of Medical Sciences, 6Advanced ICT Research Institute, National Institute of Information and Communications Technology, 7Dept. of2Pos176

Pathology, Osaka City Univ. Graduate School of Medicine, 8Institute of Scientific and Industrial Research, Osaka Univ.)
マウス Tppp (Tubulin Polymerization Promoting Protein)の機能解析
Analysis of Tppp (Tubulin Polymerization Promoting Protein)
Masahiro Kawakita1, Arashi Seki1, Katsuyoshi Takaoka2, Hiroshi Hamada3, Kyosuke Shinohara1 (1Tokyo University of Agriculture & Technology,
2EMBL Heidelberg, 3RIKEN Center for Developmental Biology)

2Pos177

マウス繊毛細胞における Dpcd の機能解析
Role of Dpcd in motile cilia of mice
Misato Tamegai1, Mahito Kikumoto2, Miki Kinoshita3, Akihiro Kawamoto3, Keiichi Namba3, Hiroshi Hamada4, Katsumi Imada3, Akihiro Narita2,

2Pos178

2Pos179

Kyosuke Shinohara1 (1Tokyo University of Agriculture & Technology, 2Nagoya University, 3Osaka University, 4RIKEN Center for Developmental
Biology)
IFT（繊毛内輸送）に関与する基底小体微小管の機能に関する研究
The function of the basal body microtubules associated with intraflagellar transport (IFT)
Yurika Koiso, Shin Yamaguchi, Mitsuhiro Sugawa, Takuya Kobayashi, Yoko Y. Toyoshima, Junichiro Yajima (Department of Life Sciences
Graduate School of Art & Sciences, The University of Tokyo)
Distinctive structured radial spoke of mouse sperm underlies wave propagation of flagella
Kaoru Horiuchi1, Hironori Ueno2, Akihiro Narita3, Hiroshi Hamada4, Kyosuke Shinohara1 (1Tokyo University of Agriculture and Technology,

2Pos180

2Aichi University of Education, 3Nagoya University, 4RIKEN Center for Developmental Biology)
クラミドモナス鞭毛から精製したラジアルスポークの特性
Properties of the Purified Radial Spoke of Chlamydomonas Flagella2Pos181

Hitoshi Sakakibara1, Yosuke Shimizu1, Pinfen Yang2, Hiroaki Kojima1 (1Protein Biophys. Gr., NICT, 2Dept. Biol. Sci., Marquette Univ.)
ハプトネマの微小管系急速コイリング運動メカニズムを探る
Unveiling a mechanism for rapid microtubule coiling movement of haptonema
Mami Nomura1, Keiko Hirose2, Kogiku Shiba1, Kazuo Inaba1 (1Shimoda Marine Research Center, University of Tsukuba., 2Biomedical ResearchInstitute, National Institute of Advanced Industrial Science and Technology)– S111 –2Pos182

2Pos183cAMP シグナルリレーにおける膜電位変化の計測と制御
Measurement and control of membrane potential changes in cAMP signal relay
Yusuke V. Morimoto1, Masahiro Ueda1,2 (1QBiC, RIKEN, 2Grad. Sch. Frontier Biosci., Osaka Univ.)
動的な場における時間空間知覚メカニズムの解析：走化性パラドクスの克服と細胞の整流作用
Delineating temporal and spatial sensing in migrating cells: chemotactic wave paradox and rectification of the leading edge response
Akihiko Nakajima1, Shuji Ishihara2, Motohiko Ishida3, Daisuke Imoto3, Satoshi Sawai1,3 (1Res. Cent. Comp. Sys. Biol., Grad. Sch. Arts Sci., Univ.

2Pos184

2Pos185

2Pos186

Tokyo, 2Sch. Sci. Tech., Meiji Univ., 3Dept. Basic Sci., Grad. Sch. Arts Sci., Univ. Tokyo)
細胞か接着により誘導される細胞の集団運動
Cell-Cell Adhesion guiding Collective Cell Migration
Katsuyoshi Matsushita (Department of Biological Sciences, Graduate School of Science, Osaka University)
力測定で明かす神経幹細胞の集団遊走
Measuring the Forces in Neural Stem Cell Monolayer
Masahito Uwamichi, Masaki Sano (Dept. of Phys., The Univ. of Tokyo)
細胞性粘菌における集団的回転運動の定量解析
Quantitative analysis of collective rotational motion of Dictyostelium cells
Taihei Fujimori1, Akihiko Nakajima1,2, Ryo Yokota3, Ryo Nakabayashi4, Gen Honda1, Tetsuya Kobayashi3, Satoshi Sawai1,2 (1Grad. Sch. of Arts

2Pos187& Sci., Univ. Tokyo, 2Res. Ctr. Complex Syst. Biol., Univ. Tokyo, 3Inst. of Ind. Sci., Univ. Tokyo, 4Univ. Tokyo)
Spatial heterogeneous and transient dynamics during collective cell migration in a monolayer of MDCK epithelial cells
Preetom Nag1, Helal Khalifa1,2, Hiroshi Teramoto3, Naoya Yamaguchi4, Chun-Biu Li1, Hisashi Haga2, Tamiki Komatsuzaki1,2 (1Research Institutefor Electronic Science, Hokkaido University, 2Graduate School of Life Science, Hokkaido University, 3Hitachi, Ltd. Research & Development

Group, 4Skirball Institute of Biomolecular Medicine, New York University Langone Medical Center, USA)細胞の協調運動における接着結合タンパク質の役割
Roles of adherence junction proteins in the collective cell movement in vitro and vivo
Takeomi Mizutani, Kazushige Kawabata (Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido
University)
2Pos189* 筋分化 C2C12 におけるインスリン刺激時 S6K 活性のダイナミクスと不均一性
Dynamics and Heterogeneity of S6K activity in insulin stimulated-C2C12 myotubes

2Pos188

Haruki Inoue1, Katsuyuki Kunida2, Daisuke Hoshino2, Takumi Wada2, Shinya Kuroda1,2 (1Grad. Frontier Sci., Univ. Tokyo, 2Grad. Sch. Sci., Univ.
Tokyo)
2Pos190 Beating rate changes of isolated cardiomyocyte clusters in different thermal environments
Wei Wang, Tomoyuki Kaneko (LaRC, Dept. Frontier Biosci., Hosei Univ.)
改良型蛍光ATP センサーを用いた一細胞及び細胞内局所 ATP 濃度の測定
2Pos191
Quantification of single-cell and subcellular ATP concentrations using an improved fluorescent ATP indicator in mammalian cells
Hideyuki Yaginuma, Yasushi Okada (QBiC, RIKEN)
2Pos192* がん細胞の損傷回復過程の定量評価
Quantitative evaluation of recovery from damage in cancer cells
Morito Sakuma1,2, Kazuhito Tabata1,2, Hiroyuki Noji2, Hideo Higuchi1 (1Department of Physics, Graduate School of Science, The University ofTokyo, 2Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, 3JSPS Research Fellow)
2Pos193* ナノ秒パルス電場による細胞内応答の顕微ラマン・蛍光分光法を用いたその場観測
In situ observation of the intracellular responses to nanosecond pulsed electric fields by Raman and fluorescence spectroscopy
Yusuke Horii, Hirotsugu Hiramatsu, Takakazu Nakabayashi (Graduate School of Pharmaceutical Sciences, Tohoku University)
2Pos194* ミトコンドリア輸送・膜電位・ATP と神経伸展の相関解析
Correlation analysis of transport, membrane potential, and ATP levels of mitochondria and neurite extension
Rika Suzuki, Kohji Hotta, Kotaro Oka (Keio Univ.)
2Pos195* 細胞内局所発熱がストレス顆粒形成を開始する
Intracellular local thermogenesis initiates stress granule formation
2Pos1962Pos197
2Pos198

Beini Shi1, Kohki Okabe1,2, Takashi Funatsu1 (1Grad. Sch. Pharm. Sci., Univ. Tokyo, 2PRESTO, JST)
Investigating the contribution of cytoskeletons on intracellular temperature variation
Takashi Yanagi1, Kohki Okabe1,2, Takashi Funatsu1 (1Grad. Sch. Pharmac. Sci., Univ. Tokyo, 2JST, PRESTO)
Attempt to Detect Functional Interaction between FoF1-ATPase and Adenine Nucleotide Translocator
Saki Yamashita, Takahiro Shibata, Yoshihiro Ohta (Div. of Biotech. And Life Sci., Inst. of Eng., Tokyo Univ. of Agr. and Tech.)
Effects of mitochondrial volume on the generation of reactive oxygen species
Sawako Kimura1, Satoshi Honda2, Norihiro Umeda2, Yoshihiro Ohta1 (1Div. of Biotech. And Life Sci., Inst. of Eng., Tokyo Univ. of Agr. and Tech.,
2Dept. of Mech. Sys. Eng., Tokyo Univ. of Agr. and Tech.)2Pos199
2Pos200

Partial contribution of mitochondrial permeability transition to t-butyl hydroperoxide-induced cell death
Naoko Ashida, Xiaolei Shi, Hyonjin Choi, Yoshihiro Ohta (Div. of Biotech. And Life Sci., Inst. of Eng., Tokyo Univ. of Agr. and Tech.)
Monitoring of mitochondrial activity during cell division
Kyunghak Cho, Kotoe Hirusaki, Yoshihiro Ohta (Div. of Biotech. And Life Sci., Inst. of Eng., Tokyo Univ. of Agr. and Tech.)– S112 –生体膜・人工膜：情報伝達 / Biological & Artificial membrane: Signal transduction
2Pos201* パターン化人工膜を用いて脂質ラフトによる光シグナル伝達の制御機構を解明する
Regulation of phototransduction by lipid rafts studied with a micropatterned model membrane
Yasushi Tanimoto1, Sakiko Kojima1, Fumio Hayashi2, Kenichi Morigaki1,3 (1Grad. Sch. Agri, Univ. Kobe, 2Grad. Sch. Scie, Univ. Kobe, 3Biosignal
2Pos202

Research Center, Univ. Kobe)
全反射照明蛍光顕微鏡を用いた RalGDS 分子の EGF 依存的な膜局在化メカニズムの解明
Elucidation of the EGF dependent localization mechanism of RalGDS molecule to plasma membrane using TIRF microscopy
Ryo Yoshizawa1,2, Nobuhisa Umeki2, Masataka Yanagawa2, Masayuki Murata1, Yasushi Sako2 (1Grad. Sch. Sci., Univ. Tokyo, 2Wako Inst., Riken)

生体膜・人工膜：ダイナミクス / Biological & Artificial membrane: Dynamics
分子動力学シミュレーションによる膜の細孔形成自由エネルギー解析
Free energy analysis of membrane pore formation by molecular dynamics simulations
Yusuke Miyazaki, Wataru Shinoda, Susumu Okazaki (Grad. Eng., Univ. Nagoya)
2Pos204* Min 反応拡散波の油中水滴内再構成
Reconstitution of Min Reaction-Diffusion Waves in Water-in-Oil Microdroplets
Shunshi Kohyama, Nobuhide Doi, Kei Fujiwara (Grad. Sch. Sci. Tech., Keio Univ.)
2Pos205 ラクトフェリシン B フラグメントと大腸菌および GUV との相互作用
Interactions of a fragment of lactoferricin B with E.coli and single GUVs

2Pos203

Md. Moniruzzaman1, Hideo Dohra2, Masahito Yamazaki1,3,4 (1Grad. Sch. Sci. Tech., Shizuoka Univ., 2Res. Inst. Green Sci. Tech., Shizuoka
2Pos206

University, 3Res. Inst. Ele., Shizuoka Univ., 4Grad. Sch. Sci., Shizuoka Univ.)
フォトクロミック脂質を用いた蛍光脂質のダイナミクス
Intermembrane transfer of fluorescent lipid analogs using photochromic lipid analogs as FRET acceptors
Mariko Sumi1,2, Asami Makino1, Takehiko Inaba1, Fumihiro Fujimori2, Peter Greimel1, Toshihide Kobayashi1,3 (1Lipid Bio. Lab., RIKEN, 2Grad.

2Pos207

2Pos208

2Pos209

Sch. of Humanities and Life Sci., Tokyo Kasei Univ., 3CNRS, France)
脂質膜の力学的特性は細胞透過ペプチド・トランスポータン 10(TP10)の単一ベシクルへの侵入に影響を与える
Mechanical Properties of Lipid Bilayers Affect the Entry of Cell-Penetrating Peptide Transportan 10 (TP10) into Single Vesicles
Md. Zahidul Islam1, Sabrina Sharmin1, Masahito Yamazaki1,2,3 (1Grad. Sch. Sci. Tech., Shizuoka Univ., 2Res. Inst. Ele., Shizuoka Univ., 3Grad.
Sch. Sci., Shizuoka Univ.)
一定張力が誘起する膜破壊の活性化エネルギーを用いた解析
Analysis of Constant Tension-Induced Rupture of Lipid Membranes Using Activation Energy
Md. Abu Sayem Karal1, Victor Levadnyy1,3, Masahito Yamazaki1,2 (1Shizuoka Univ. Grad. Sch. Sci. Tech., 2Shizuoka Univ. Res. Inst. Ele., 3Rus.
Acad. Sci.)
抗菌ペプチド・マガイニン２が脂質膜中に誘起するポア形成のメカニズム
A Mechanism of Antimicrobial Peptide, Magainin 2-Induced Pore Formation in Lipid Membranes
Moynul Hasan1, Mohammad Abu Sayem Karal1, Victor Levadnyy1,2, Md. Zahidul Islam1, Masahito Yamazaki1,3,4 (1Grad. Sch. Sci. Tech., Shizuoka

2Pos210

Univ., 2Rus. Acad. Sci., 3Res. Inst. Ele., Shizuoka Univ., 4Grad. Sch. Sci., Shizuoka Univ.)
引張下コレステロール含有リン脂質二重膜における指組み構造の形成：分子動力学シミュレーション
Stretch-Induced Interdigitated Phase Formation in Phospholipid/Cholesterol Bilayer: Molecular Dynamics Simulation

2Pos211

Taiki Shigematsu1, Kenichiro Koshiyama2, Shigeo Wada2 (1JAMSTEC, 2Grad. Sch. Eng. Sci., Osaka Univ.)
細胞サイズ液滴内における高分子溶液の拡散とその空間閉じ込めの影響
Diffusion in polymer solutions confined in cell-sized droplets: effect of confinement size

2Pos212

Chiho Watanabe1, Miho Yanagisawa2 (1Inst. Glob. Innov., Tokyo Univ. Agri. & Tech., 2Grad. Sch. Tech., Tokyo Univ. Agri. & Tech.)
Investigating interactions and dynamics of pleckstrin homology domains on a lipid membrane surface
Eiji Yamamoto1, Antreas C. Kalli2, Takuma Akimoto1, Mark S.P. Sansom2, Kenji Yasuoka3 (1Grad. Sch. Sci. Technol., Keio Univ., 2Dept.
Biochem., Univ. Oxford, 3Dept. Mech. Eng., Keio Univ.)

生体膜・人工膜：構造・物性 / Biological & Artificial membrane: Structure & Property
2Pos213* D 体フェニルアラニンを含む抗菌ペプチド Phenylseptin の脂質膜との相互作用解析
Analysis of interaction between antimicrobial peptide phenylseptin containing a D-phenylalanine and membrane
Yuta Matsuo, Izuru Kawamura (Grad. Sch. Eng., Yokohama Natl. Univ.)
2Pos214* 人工生体膜とナノ空間を利用した 1 分子計測技術の開発
Single-molecule observation technique based on a model membrane and a nanometric gap structure
Koji Ando1, Humio Hayashi2, Kenichi Morigaki1,3 (1Grad. Agri., Univ. Kobe, 2Grad. Sci., Univ. Kobe, 3Biosignal. Univ. Kobe)
2Pos215* カチオン性抗菌ペプチド ボンビニン H2 および H4 のリーシュマニア原虫模倣膜との特異的な相互作用
Specific interaction of cationic antimicrobial peptides bombinin H2 and H4 with Leishmania protozoa mimetic membrane
Shiho Kaneda, Akira Naito, Izuru Kawamura (Grad. Sch. Eng., Yokohama Natl. Univ.)– S113 –2Pos216

2Pos217

水の脂質膜透過に対する膜の張力の効果
Effect of Lateral Tension on Membrane Permeability of Water in Lipid Membranes
Sayed Ul Alam Shibly1, Masahito Yamazaki1,2,3 (1Grad. Sch. Sci. Tech., Shizuoka Univ., 2Res. Inst. Ele., Shizuoka Univ., 3Grad. Sch. Sci., Shizuoka
Univ.)
部分フッ素化リン脂質からなる人工膜内色素分子の発光挙動
Investigation of fluorescence emission from dye-lipid in partially fluorinated lipid bilayer
Toshinori Motegi1, Ryugo Tero2, Toshiyuki Takagi3, Hiroshi Takahashi1, Hideki Amii1, Toshiyuki Kanamori3, Masashi Sonoyama1 (1Gunma

2Pos218

Univ., 2Toyohashi Univ. Tech., 3AIST)
パターン化人工膜を利用した NAP-22 の膜結合と凝集挙動解析
Membrane binding and aggregation of neuronal acidic protein of 22kDa (NAP-22) studied with a patterned model membrane
Sakiko Kojima1, Yasushi Tanimoto1, Fumio Hayashi3, Shohei Maekawa3, Kenichi Morigaki1,2 (1Grad. Sch. Agri., Univ. Kobe, 2Biosignal Research

2Pos219

2Pos220

Center, Univ. Kobe, 3Grad. Sch. Sci., Univ. Kobe)
Phospholipase C and D induced defects in POPC and POPC: POPG lipid bilayers: A simulation study
M Harunur Rashid1, M N Holme2, M M Stevens2, I Yarovsky1 (1School of Engineering, RMIT university, Melbourne, Australia, 2Department ofMaterials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College, London, UK.)
固体 NMR と MD シミュレーションによる抗菌ペプチドアラメチシンとメリチンの膜結合構造と配向の解明
Structure and orientation of antimicrobial peptides alamethicin and melittin in membrane revealed by solid-state NMR and MD simulation
Akira Naito1, Takashi Nagao1, Daisuke Mishima1, Namsrai Javkhalantugs2, Jun Wang1, Daisuke Ishioka1, Kiyonori Yokota1, Kazushi Norisada1,

2Pos222

Izuru Kawamura1, Kazuyoshi Ueda1 (1Grad. Schl. Eng. Yokohama Natl. Univ., 2Schl. Eng. and Appl. Sci., Natl. Univ. Mongolia)
巨大一枚膜ベシクルに内包された DNA コンピュータ基盤遺伝子発現制御システム
Development of a DNA computer-based gene-regulatory system encapsulated in a giant unilamellar vesicle

2Pos223

Koh-ichiroh Shohda1, Toru Nishikata1, Yutetsu Kuruma2, Akira Suyama1 (1Grad. Sch. Arts and Sciences, The University of Tokyo, 2Earth-Life
Science Institute, Tokyo Institute of Technology)
Local pressure tensor calculation for molecular simulations and its application to lipid membranes
Koh Nakagawa, Hiroshi Noguchi (ISSP, Univ. of Tokyo)
Formation of vesicles using self-reproducing oil droplet system

2Pos224

Kensuke Kurihara1,2,3 (1Okazaki Institute for Integrative Bioscience, 2Institute for Molecular Science, 3Research Center for Complex Systems
Biology, The Univ. of Tokyo)
2Pos225* アクチンフィラメントの細胞膜シート上への結合
The binding of actin filament on the cell membrane flat sheet
Shun Wakamatsu1, Kuniyuki Hatori1, Takashi Okuno2 (1Grad. Sch. Sci. & Eng., Yamagata Univ., 2Fac. Sci., Yamagata Univ.)
2Pos226* アクチン線維を封入した巨大リポソームの形態変化
Shape change of giant liposomes encapsulating actin filaments
Shunsuke Tanaka, Masahito Hayashi, Kingo Takiguchi (Grad. Sch. Sci., Nagoya Univ.)

光生物：視覚･光受容 / Photobiology: Vision & Photoreception
2Pos227

全反射赤外分光法を用いたウシオプシンと匂い分子の相互作用研究
ATR-FTIR study on the interactions between bovine opsin and odorants

Kunisato Kuroi1, Takefumi Morizumi2, Hisao Tsukamoto1, Oliver P Ernst2, Yuji Furutani1 (1Inst. for Mol. Sci., 2Univ. Toronto)
2Pos228 Glu381Lys 点変異体を用いたニワトリクリプトクロム 4 の光反応メカニズムの解析
Photoreaction mechanism of chicken cryptochrome4 studied by using a Glu381Lys mutant
Hiromasa Mitsui, Kota Miura, Keiko Okano, Toshiyuki Okano (Dept. Eng. and Biosci., Grad. Sch. Adv. Sci. and Eng., Waseda Univ.)
2Pos229* ニワトリクリプトクロム４の光依存的な相互作用分子とその分子メカニズム
Identification of chicken CRY4-interacting molecules and the interaction mechanism
Ayano Orii, Shingo Kondo, Keiko Okano, Toshiyuki Okano (Dept. Eng. and Biosci., Grad. Sch. Adv. Sci. and Eng., Waseda Univ.)
2Pos230 桿体視細胞に発現する視物質の熱活性化頻度
Thermal activation rates of visual pigments expressed in rods
Keiichi Kojima1, Yuki Matsutani1, Masataka Yanagawa2, Takahiro Yamashita1, Yasushi Imamoto1, Osamu Hisatomi3, Yumiko Yamano4, Akimori
Wada4, Yoshinori Shichida1 (1Grad. Sch. Sci., Kyoto Univ., 2Cell. Info. Lab., Riken, 3Grad. Sch. Sci., Osaka Univ., 4Kobe Pharm. Univ.)
トランスデューシンは PDE を“間接的に”活性化する
Transducin activates cGMP phosphodiesterase indirectly
Teizo Asano, Shuji Tachibanaki, Satoru Kawamura (Grad. Sch. Frontier Biosci., Osaka Univ.)
2Pos232* 低温赤外分光法によるサル緑感受性視物質がもつ塩化物イオン結合部位の構造解析
Structural analysis of chloride binding site of monkey green studied by light-induced difference FTIR spectroscopy
2Pos231

Shunta Nakamura1, Kota Katayama2, Hiroo Imai3, Hideki Kandori1 (1Grad. Sch. Eng., Nagoya Inst. Tech., 2Dept. Pharm CWRU, USA, 3Primate
Res Inst., Kyoto Univ.)– S114 –2Pos233

新口動物の光受容タンパク質 Opn5 の多様性
Diversity of the photoreceptor protein Opn5 found in deuterostomes
Takahiro Yamashita1, Ikutaro Sawada1, Keita Sato2, Naoaki Sakamoto3, Keisuke Takahashi1, Naoyuki Iwabe1, Hideyo Ohuchi2, Takashi

2Pos234

Yamamoto3, Yoshinori Shichida1 (1Grad. Scl. of Sci., Kyoto Univ., 2Okayama Univ. Grad. Sch. of Med., 3Grad. Scl. of Sci., Hiroshima Univ.)
Light-dependent association and dissociation of arrestin with bistable opsins
Takashi Nagata1, Mitsumasa Koyanagi1, 2, Emi Yamashita-Kawano1, Robert Lucas3, Akihisa Terakita1 (1Graduate School of Science, Osaka City

2Pos235

2Pos236

2Pos237

University, 2JST PRESTO, 3Faculty of Life Sciences, The Unversity of Manchester)
桿体アレスチンのスプライスバリアント・p44 の自己会合の解析
Self-association of p44, a splice variant of visual rod arrestin
Yasushi Imamoto1, Keiichi Kojima1, Toshihiko Oka2, Takahiro Yamashita1, Yoshinori Shichida1 (1Grad. Sch. Sci., Kyoto Univ., 2Grad. Sch. Sci.,
Shizuoka Univ.)疾患に関わるロドプシン変異体の FTIR 研究
FTIR study of disease-causing mutations of rhodopsin
Akiko Enomoto1, Shunta Nakamura1, Kota Katayama2, Hiroo Imai3, Hideki Kandori1 (1Nagoya Inst. Tech., 2Dept. Pharm., CWRU, USA, 3Primate
Res. Inst., Kyoto Univ.)
N-terminal region of modified Volvox channel rhodopsin-1(mVChR1) enchances Na+ Influx by drowing hydrogen ion
Yuko Sakajiri1, Kanako Hara2, Yoshito Watanabe2, Tetsuya Sakajiri3, Eriko Sugano2, Hiroshi Tomita1,2 (1Ugas. Agr. Iwate Univ., 2Se. Iwate Univ.,
3Fac. of Nutr. Sci., Morioka Univ.)

2Pos238

フグ眼球由来の細胞株における広範囲な光波長応答性
A wide-range spectral photosensitivity in the puffer fish ocular cells
Keiko Okano1, Shoichi Ozawa1, Hayao Sato1, Sawa Kodachi1, Masaharu Ito1, Toshiaki Miyadai2, Akihiro Takemura3, Toshiyuki Okano1 (1Dept.

2Pos239

Eng Biosci, Grad. Sch. Adv Sci. and Eng, Waseda Univ., 2Fac Marine Biosci, Fukui Pref. Univ., 3Dept. Chem Biol. & Marine Sci. Fac Sci., Univ.
Ryukyus)
Guillardia theta 由来ロドプシン様タンパク質の分子機能解明
Molecular functions of rhodopsin-like proteins from Guillardia theta

Yumeka Yamauchi1, Masae Konno1, Keiichi Inoue1,2, Satoshi Tsunoda1, Hideki Kandori1 (1Nagoya Inst. tech., 2JST. PRESTO)
2Pos240* 海洋性細菌のもつ光駆動イオンポンプ
Light-driven ion-pump activity of native marine bacteria
Yuichi Hashimoto1, Rei Abe-Yoshizumi1, Yoshitaka Kato1, Keiichi Inoue1,2, Hideki Kandori1 (1Nagoya Institute of Technology, 2JST PRESTO)

光生物：光合成 / Photobiology: Photosynthesis
2Pos241

好熱性紅色光合成細菌 Alc. tepidum 由来の光捕集複合体の単離精製と分光学的特性評価
Purification and spectroscopic study of the light-harvesting complexes from thermophilic purple bacterium Allochromatium tepidum
N. Nakamura1, S.-W. Lu2, A. Ohkoshi1, K. Okazaki1, T. Kawakami1, M. T. Madigan3, Y. Kimura2, S. Otomo1 (1Ibaraki Univ., 2Grad. Sch. Agri.

2Pos242

Sci., Kobe Univ., 3Southern Illinois Univ.)
Thermochromatium tepidum 由来光捕集１複合体における部位特異的変異体の分光学的解析
Spectroscopic characterization of site-directed mutants in Light-Harvesting 1 complex from Thermochromatium tepidum
Kanako Hashimoto1, Seiji Akimoto2, Kenji Nagashima3, Takashi Ohno1, Sheiu Otomo4, Yukihiro Kimura1 (1Graduate school of Agriculture, Kobe
University, 2Graduate school of Science, Kobe University, 3Research Institute for Photobiological Hydrogen Production, Kanagawa University,
4Faculity of Science, Ibaraki University)

2Pos243

光合成光捕集複合体における金属イオン認識の構造基盤
Structural basis for the metal-ion recognition of the bacterial core light-harvesting complex
T. Kawakami1, L.-J. Yu2,3, Y. Kimura4, S. Otomo2 (1Grad. Sch. Sci. Eng., Ibaraki Univ., 2Ibaraki Univ., 3Present address: Grad. sch. Boi.,
Okayama Univ., 4Grad. Sch. Agri. Sci., Kobe Univ.)

2Pos244

NMR study of the interaction on the two ferredoxin isoforms with ferredoxin-NADP+ reductase
Risa Mutoh1,2, Akane Furuya2,3, Takahisa Ikegami4, Michael Hippler5 (1Faculty of Sci., Fukuoka Univ., 2Inst. for Protein Research, Osaka Univ.,
3Grad. Sch. of Sci., Osaka Univ., 4Grad. Sch. of Med. Life Sci., Yokohama City Univ., 5Inst. for Biology and Biotechnology, Univ. of Munster)2Pos245* 同一メナキノン分子の酸化還元電位が２種の光合成反応中心蛋白質で 500mV も異なる理由
Redox potential difference of 500 mV for menaquinones in two types of photosynthetic reaction centers2Pos246

Keisuke Kawashima1, Hiroshi Ishikita1,2 (1Grad. Sch. Eng., Univ. of Tokyo, 2RCAST, Univ. of Tokyo)
ヘリオバクテリア光合成反応中心の過渡吸収変化と低温蛍光解析
Analyses of transient absorption changes and low-temperature fluorescence in the photosynthetic reaction center of heliobacteria

2Pos247of Life Science, Ritsumeikan University, 3Institute for Protein Research, Osaka University, 4Center for Gene Research, Nagoya University)
Magnetic structure of reduced [2Fe-2S] Rieske cluster from green sulfur bacteria Chlorobaculum tepidum studied by ESEEM
Naotaka Terashima (photobioenergetics lab, graduate school of science, Nagoya university)

Hirozo Oh-oka1, Risa Kojima1, Chihiro Azai2, Risa Mutoh3, Genji Kurisu3, Shigeru Itoh4 (1Graduate School of Science, Osaka University, 2College– S115 –2Pos248

好熱性紅色光合成細菌 Thermochromatium tepidum 由来反応中心複合体におけるカルシウムイオンの機能的、構造的役割
Functional and structural roles of calcium ion in the reaction center from thermophilic purple bacterium, Thermochromatium tepidum
Michie Imanishi1, Masayuki Kobayashi2, Manami Kobayashi1, Mari Matsuzaki3, Yuki Yura3, Takashi Ohno3, Seiu Otomo4, Yukihiro Kimura3

2Pos249(1Faculty of Agriculture, Kobe University, 2Ariake National College of Technology, 3Guraduate school of Agriculture, Kobe university, 4Faculty of
Science, Ibaraki University)
Initial formation of the radical pair in reaction center complex of Heliobacterium modesticaldum detected by transient ESR
Hiroyuki Tsukuno1, Risa Mutoh3, Genji Kurisu2,4, Hirozo Oh-oka2, Hiroyuki Mino1 (1Grad. Sch. Sci., Nagoya Univ., 2Grad. Sch. Sci., Osaka

Univ., 3Dept. Applied Phys. Fac. Sci., Fukuoka Univ., 4Ins. Pro. Res., Osaka Univ.)
2Pos250* フラビンタンパク質で目指す人工光合成
Artificial photosynthesis based on the engineered flavoprotein LOV
Nozomi Ueda, Yukiko Ono, Tatsuya Iwata, Masayo Iwaki, Hideki Kandori (Nagoya Institute of Technology)
2Pos251* 分子動力学シミュレーションによる光捕集複合体の自己組織化過程に関する理論的研究
Theoretical study on the self-organization process of the light-harvesting complexes with molecular dynamics simulation
Marie Yamauchi1, Shinji Saito2,3, Masahiro Higashi4 (1Graduate School of Engineering and Science, University of the Ryukyus, 2Department ofTheoretical and Computational Molecular Science, Institute for Molecular Science, 3The Graduate University for Advanced Studies (SOKENDAI),
4Faculty of Science, University of the Ryukyus)

光生物：光遺伝学･光制御 / Photobiology: Optogenetics & Optical Control
2Pos252

2Pos253

クリプト藻由来のカチオンチャネルロドプシンのイオン透過メカニズムの電気生理学による研究
Electrophysiological study of cation channelrhodopsins from cryptophyte algae
Satoshi Tsunoda1, Yumeka Yamauchi1, Masae Konno1, Keiichi Inoue1,2, Hideki Kandori1 (1Grad. Sch. Sci., Nagoya Inst. of Tech., 2JST, PREST)
アニオンチャネルロドプシン 2 の光開閉型 Cl-/H+対向輸送活性A light-dependent Cl-/H+ antiport activity in anion channelrhodopsin-2
Satoko Doi1, Takashi Tsukamoto1, Srikanta Chowdhury2, Susumu Yoshizawa3, Akihiro Yamanaka2, Yuki Sudo1 (1Grad. Sch. of Med. Dent. &2Pos254

Pharm. Sci., Okayama Univ., 2RIEM, Nagoya Univ., 3AORI, Univ. of Tokyo)
サーモフィリックロドプシンの耐熱性・高光遺伝学活性の構造基盤
Structural basis for high thermal stability and efficient optogenetic function of thermophilic rhodopsin
Takashi Tsukamoto1, Kenji Mizutani2, Taisuke Hasegawa3, Megumi Takahashi4, Naoya Honda1, Naoki Hashimoto2, Kazumi Shimono5, Seiji

2Pos255

2Pos256

Miyauchi5, Shin Takagi4, Shigehiko Hayashi3, Takeshi Murata2, Yuki Sudo1 (1Okayama Univ., 2Chiba Univ., 3Kyoto Univ., 4Nagoya Univ., 5Toho
Univ.)
新規酵素ロドプシンの機能解析
Characterization of a novel enzyme rhodopsin
Kazuho Yoshida1, Satoshi Tsunoda1, Leonid S. Brown2, Hideki Kandori1 (1Nagoya Inst. Tech., 2Univ. Guelph)
ユニークな光反応を示す微生物型ロドプシンの研究
Microbial rhodopsins with unique photoreaction
Yoshitaka Kato, Keiichi Inoue, Shota Ito, Satoshi Tsunoda, Yurika Nomura, Hideki Kandori (Grad. Sch. Eng., Nagoya Inst. Tech.)

生命の起源･進化 / Origin of life & Evolution
2Pos257* マイクロデバイスと大腸菌の融合を基とした人工細胞の開発
A step towards creating life: Development of the hybrid cell based on the fusion of micron-scaled device and E. coli
Yoshiki Moriizumi1,2, Kazuhito V. Tabata1,2,3, Rikiya Watanabe1,3, Tomohiro Doura4, Mako Kamiya3,4, Yasuteru Urano4,5,6, Hiroyuki Noji1,2(1Dept. Appl. Chem., Grad. Sch. Eng, Univ. Tokyo, 2ImPACT, Cab. Office, Gov. Japan, 3PRESTO, JST, 4Grad. Sch. Med., Univ. Tokyo, 5Grad. Sch.
Pharm, Univ. Tokyo, 6CREST, AMED)
2Pos258* 翻訳と共役した再帰的 DNA 複製システムの確立
The establishment of translation-coupled recursive DNA replication system
2Pos259

Yoshihiro Sakatani1, Norikazu Ichihashi1,2 (1Grad. Sch. Info., Osaka Univ., 2Grad. Sch. Bio., Osaka Univ.)
Characterization on Escherichia coli L-Form
Shino Toe1, Kazuhito V. Tabata2,3,4, Yoshiki Moriizumi2,3, Hiroyuki Noji2,3 (1Dept. Appl. Chem., UG. Sch. Eng, Univ. Tokyo, 2Dept. Appl. Chem.,

Grad. Sch. Eng, Univ. Tokyo, 3ImPACT, Cab. Office, Gov. Japan, 4PRESTO, JST)
対称性の自発的破れによる遺伝子の起源
2Pos260
The origin of genes through spontaneous symmetry breaking
Nobuto Takeuchi (Univ. Tokyo, Grad. Sch. of Arts and Sciences)2Pos261* 円偏光による L 型アミノ酸過剰生成機構の理論的探求
Theoretical investigation of the generation of L-form amino acid excess by the CPL irradiation
Akimasa Sato1, Mitsuo Shoji2, Katsumasa Kamiya3, Kenji Shiraishi4, Kazuhiro Yabana2, Yasuteru Shigeta2, Masayuki Umemura2 (1Grad. Sch.
Pure. App. Sci., Univ. Tsukuba, 2Center Comp. Sci., Univ. Tsukuba, 3Center Basic Edu. Integ. Learn., Kanagawa Inst. Tech., 4Inst. Mat. Sys. Sust.,
Nagoya Univ.)– S116 –ゲノム生物学：ゲノム構造 / Genome biology: Genome structure
2Pos262

2Pos263

3D ゲノム構造の集団ベースモデリングPopulation-based framework of 3D genome modelingTakeshi Sugawara (RcMcD, Hiroshima University)
Dynamic chromatin domains revealed by super-resolution live-cell imaging
Tadasu Nozaki1, Sachiko Tamura1, Ryosuke Imai1, Tomomi Tani2, Masaru Tomita3, Takeharu Nagai4, Yasushi Okada5, Kazuhiro Maeshima1

2Pos264(1Natl. Inst. Genet., 2MBL, 3Inst. Adv. Biosci., Keio Univ., 4ISIR, Osaka Univ., 5QBiC, RIKEN)
分裂酵母クロマチン動態の網羅的解析
Comprehensive Analysis of Chromatin Dynamics in Fission Yeast
Toshinori Namba1, Sayaka Suzuki2, Takeshi Sugawara1, Da-Qiao Ding3, Yasushi Hiraoka4, Yuichi Togashi1, Masaru Ueno5, Shin-ichi Tate1(1RcMcD, Hiroshima Univ., 2Dept. of Math. and Life Sci., Hiroshima Univ., 3Adv. ICT Res. Inst., NICT, 4Dept. of Biol. Sci., Osaka Univ., 5Grad.
Sch. of Adv. Sci. of Matter, Hiroshima Univ.)

バイオインフォマティクス：構造ゲノミクス / Bioinformatics: Structural genomics
2Pos265

2Pos266
2Pos267

2Pos268

長さの異なる塩基配列組み合わせの頻度・分布を用いたヌクレオソーム配置推定
Predict nucleosome positioning by incorporating the frequencies and distributions of three length-different nucleotide segments
Akinori Awazu1,2 (1Dept. of Math. and Life Sciences, Hiroshima Univ., 2RcMcD , Hiroshima Univ.)
New rules of protein structures
Shunsuke Nishiyama1, Shintaro Minami2, George Chikenji1 (1Dept. of Comp. Sci. & Eng., Nagoya Univ., 2Grad. Sch. of Inf. Sci., Nagoya Univ.)
全原子 Motion Tree による構造変化の解析
Description of protein structural changes by full-atom Motion Tree
Ryotaro Koike (Grad. Sch. of Info. Sci., Nagoya Univ.)
分子動力学法を用いた、ポリグルタミン酸のアンフォールドダイナミクス
Unfolding dynamics of poly-glutamic acid in using molecular dynamics method
Naoki Ogasawara1, Ryosuke Iwai1, Kota Kasahara2, Tetsuro Nagai3, Takuya Takahashi2 (1Grad. Sch. Life. Sci., Ritsumei. Univ., 2Col. Life. Sci.,

2Pos269

Ritsumei. Univ., 3Col. Sci., Univ. Nagoya)
MEGADOCK-Azure: Microsoft Azure クラウド環境での並列タンパク質間相互作用予測計算
MEGADOCK-Azure: High-performance protein-protein interaction predictions on Microsoft Azure HPC
Masahito Ohue1, Yuki Yamamoto1,2, Hiroyuki Sato3, Takashi Matsushita3, Yutaka Akiyama1,2 (1Sch. of Computing, Tokyo Tech., 2ACLS, Tokyo

2Pos270

Tech., 3IMSBIO Co., Ltd.)非エバルト静電ポテンシャル計算法“零多重極子和法”の開発と検証
Development and Evaluations of a Fast and Accurate Non-Ewald Electrostatic Potential Scheme, the Zero-Multipole Summation Method
Kota Kasahara1, Shun Sakuraba2, Ikuo Fukuda3, Jinzen Ikebe4, Ryuhei Harada5 (1Col. Life Sci., Ritsumeikan Univ., 2Grad. Sch. Frontier Sci., Univ.

2Pos271
2Pos272

Tokyo, 3IPR, Osaka Univ., 4QST, MMS, 5CCS, Univ. Tsukuba)
Local structures around protein phosphorylation sites
Hafumi Nishi, Kengo Kinoshita (Grad. Sch. Info. Sci., Tohoku Univ.)
膜タンパク質の構造分類：93 フォールドの同定
We found at least 93 membrane protein folds in structure classification
Tsukasa Ueno1, Masato Sakai1, Masami Ikeda2, Makiko Suwa1,2 (1Biol. Sci., Grad. Sci. Eng., Aoyama Gakuin Univ., 2Chem. Biol. Sci., Sci. Eng.,
Aoyama Gakuin Univ.)

バイオインフォマティクス：分子進化 / Bioinformatics: Molecular evolution
2Pos273

Culture-independent identification of genes encoding agarase from environmental bacteria using agarose gel microdroplets
Eiji Shigihara1, Ryo Iizuka1, Takashi Sakurai1, Yuji Hatada2, Dong Hyun Yoon3, Tetsushi Sekiguchi4, Shuichi Shoji3, Takashi Funatsu1 (1Grad.
Sch. of Pharm. Sci., Univ. Tokyo., 2Dept. of Life Sci. and Green Chem., Saitama Inst. of Technol., 3Dept. of Nanosci. and Nanoeng., Waseda Univ.,
4Res. Org. for Nano&Life Innov., Waseda Univ.)

2Pos274

人工平面脂質二重膜を用いた抗菌性ペプチドの分子進化研究
Molecular evolution of antimicrobial peptides using artificial planar lipid bilayers
Naoki Saigo1, Yusuke Sekiya2, Hirokazu Watanabe2, Ryuji Kawano3 (1Tokyo Univ. of Agri. & Tech. Dept. of Biotech. Life Sci., 2Tokyo Univ. of

2Pos275

2Pos276

Agri. & Tech. Dept. of Biotech. Life Sci., 3Tokyo Univ. of Agri. & Tech. Dept. of Biotech. Life Sci.)
タンパク質コーパスによる分散表現：ランダム配列の意味空間マッピングによる偽タンパク質の探索
Distributed representation analysis of a protein corpus: Can we identify fake proteins by mapping random sequences on a semantic space?
Hiroshi Imamura, Shinya Honda (AIST)
蛋白質構造安定性の平衡淘汰
Selection maintaining protein stability at equilibrium
Sanzo Miyazawa– S117 –計測 / Measurements
2Pos277* 原子間力顕微鏡による初期発生胚の弾性率のタイムラプスイメージング
Time-lapse imaging of elastic modulus of ascidian embryo during early development by atomic force microscopy
Yuki Fujii1, Wataru Koizumi2, Taichi Imai2, Kohji Hotta2, Kotaro Oka2, Takaharu Okajima1 (1Grad. Schl. Inform. Sci. and Tech. Hokkaido Univ.,
2Grad. Schl. Biosci. and Bioinfo. Keio Univ.)

2Pos278

信号処理蛋白質 Raf の生細胞内 ALEX 計測
In-cell ALEX measurement of cytosolic signaling protein Raf

2Pos279

Kenji Okamoto1, Kayo Hibino2, Yasushi Sako1 (1RIKEN, 2NIG)
細胞内環境におけるアンチセンス分子自己相補形成の mRNA に対する親和性への寄与
Contribution of self-complementarity of antisense molecule to the affinity for mRNA in intracellular environment

2Pos280

Shunsuke Takeda1, Kohki Okabe1,2, Takashi Funatsu1 (1Grad. Sch. Pharm. Sci., Univ. of Tokyo, 2PRESTO, JST)
顕微ラマン分光法によるバクテリア一細胞の代謝活性測定
Measuring metabolic activities in single bacterial cells by Raman microspectroscopy

2Pos281

Yota Kato1, Hiroshi Ueno1, Hiroyuki Noji1,2 (1Grad. Sch. Eng., Univ. Tokyo, 2ImPACT, JST)
上皮細胞シートの頂端膜揺らぎ：走査型イオンコンダクタンス顕微鏡
Scanning ion conductance microscopy (SICM) measurement of apical membrane fluctuation in epithelial cell monolayer

Kenta Aoki1, Ryosuke Tanaka1, Cho Nam-Joon2, Takaharu Okajima1 (1Grad. Schl. Inform. Sci. and Technol., Hokkaido Univ., 2NanyangTechnol
Univ.)
2Pos282* 蛍光偏光相関分光法により明らかになった生細胞内での分子混雑と回転拡散の関係
The relationship between rotational diffusion and crowding in living cell revealed by polarized fluorescence correlation spectroscopy

2Pos283

Makoto Oura1, Johtaro Yamamoto2, Takahiro Matsuda1, Jian Ping Gong2, Masataka Kinjo2 (1Hokkaido Univ. Grad. Sch. Life Sci., 2Hokkaido Univ.
Fac. Adv. Life Sci.)
細胞イメージングシステムを用いたナノバイオプローブの生体適合性評価
Evaluation of biocompatibility of nano-bio probes by using Cell imaging system

2Pos284

Yuko Nakane1,2, Takashi Jin2 (1Tomy Digital Biology Co., Ltd., 2RIKEN QBiC)
金ナノ粒子を用いた加熱による細胞内局所温度の制御
Manipulating the local temperature in a single cell with gold nanoparticles

2Pos285

Takaaki Honda1, Kohki Okabe1,2, Takashi Funatsu1 (1Grad. Sch. Pharma., Univ. Tokyo, 2JST PRESTO)
人工細胞開発に向けた細胞内 ATP 濃度の定量計測系開発
Development of Quantitative ATP Concentration Measurement Method in Single Cells for Artificial Cell System
Hiroki Ashikawa1, Kazuhito V. Tabata1,2,3, Hiromi Imamura4, Rikiya Watanabe1,3, Hideyuki Yaginuma5, Hiroyuki Noji1,2 (1Dept. Appl. Chem.,

2Pos286

Grad. Sch. Eng., Univ. Toyko, 2ImPACT, CAO, Govt. Japan, 3PRESTO, JST, 4Grad. Sch. Bio., Univ. Kyoto, 5QBiC, RIKEN)
バイオセンサシステムのための水素化アモルファスシリコン薄膜上のアミノ酸含有ゲルの電圧電流特性解析および蛍光性分子薄膜に関する研究
Voltage current property of amino acid containing hydrogel and molecular film on hydrogenated amorphous silicon film for biosensor
system
Makoto Horigane1, Shotaro Minato1, Hiroshi Masumoto2, Takashi Goto3, Yutaka Tsujiuchi1 (1Mat. Sci. & Eng., Akita Univ., 2Front. Res. Inst.,

2Pos287

2Pos288

Tohoku Univ., 3Inst. Mat. Res., Tohoku Univ.)
オンチップ１細胞計測系によるマクロファージの複数貪食の制御解析
Studies on regulation mechanism of multiple phagocytosis of macrophage by single cell on-chip measurement assay
Yoshiki Nakata1, Hideyuki Terazono2, Masao Odaka2, Kenji Matsuura2, Akihiro Hattori2, Kenji Yasuda1 (1Dept. Physics, Waseda Univ., 2WASEDA
Biosci. Res. Inst. Singapore (WABIOS), Waseda Univ.)
Development of Novel Scanning Microscope for Measurement of Emission and Excitation Spectra Simultaneously
Sankar Jana, Yutaka Shibata (Tohoku University)

バイオイメージング / Bioimaging
2Pos289

高速 AFM によるアクチン様細胞骨格タンパク質 MamK 繊維の直接観察
Direct observation of actin-like MamK cytoskeletal filaments by high-speed AFM
Yousuke Kikuchi1, Marina Inagawa2, Zachery Oestreicher1, Azuma Taoka1,3, Yoshihiro Fukumori1 (1Sch. of Nat. Sys., Col. of Sci. and Eng.,

2Pos290

Kanazawa Univ., 2Grad. Sch. of Nat. Sci. and Tech., Kanazawa Univ., 3Bio-AFM Center, Col. of Sci. and Eng., Kanazawa Univ.)
高速 AFM による古細菌 S. solfataricus 由来ミニ染色体維持(ssoMCM)タンパク質複合体の観察
Observation of S. solfataricus archaeal minichromosome maintenance (ssoMCM) protein complex by high-speed AFM

2Pos291

Daisuke Noshiro1, Noriyuki Kodera1,2, Toshio Ando1,3 (1Bio-AFM FRC, Inst. of Sci. & Eng., Kanazawa Univ., 2PRESTO, JST, 3CREST, JST)
ストレプトリジン O による膜孔形成の高速 AFM 観察
High-speed AFM Observation of Membrane Pore Formation by Streptolysin O
Hirotaka Ariyama1, Noriyuki Kodera1, Toshio Ando1,2 (1Bio-AFM Frontier Research Center, Kanazawa Univ., 2Dept. Phys., Kanazawa Univ.)– S118 –2Pos292

ストレス顆粒内内在性 mRNA のナノスケール蛍光イメージング
Nanoscale Fluorescence Imaging of Endogenous mRNAs in Stress Granules

2Pos293

Ko Sugawara1, Kohki Okabe1,2, Takashi Funatsu1 (1Grad. Sch. Pharm. Sci., Univ. of Tokyo, 2JST, PRESTO)
人知を超える超高速・高精度蛍光形態サイトメトリー
Ghost Cytometry: fluorescence “imaging” cytometry beyond human's limit

2Pos294

Sadao Ota1,2, Hiroyuki Noji1,3 (1Sch. Eng., Univ. Tokyo, 2JST, PRESTO, 3JST, ImPACT)
線形ゼロモード導波路を用いたアクチン重合メカニズムの 1 分子解析
Single molecule observation of actin polymerization using linear zero-mode waveguides
Soichiro Fujii1, Ryo Iizuka1, Masamichi Yamamoto1, Makoto Tsunoda1, Takashi Tanii2, Takashi Funatsu1 (1Grad. Sch. Pharm. Sci., Univ. Tokyo,
2Fac. Sci. Eng., Waseda Univ.)

T 細胞活性化における微小管動態の超解像解析
Super-resolution analysis of microtubule dynamics on T cell activation
Hengyu Shi, Yuma Ito, Wei Ming Lim, Kumiko Sakata-Sogawa, Makio Tokunaga (Sch. Life Sci. Tech., Tokyo Inst. Tech.)2Pos296 自発的光スイッチング蛍光タンパク質による簡便超解像イメージング
Simple and easy way for superresolution imaging by spontaneously switching-on fluorescent protein
Yoshiyuki Arai, Hiroki Takauchi, Takeharu Nagai (ISIR, Osaka Univ.)
2Pos297* 細胞内グルタチオンの求核付加・解離平衡に基づく超解像蛍光イメージングプローブの開発
Development of spontaneously blinking fluorophores based on nucleophilic addition of intracellular glutathione for superresolution imaging
2Pos295

Akihico Morozumi1,4, Mako Kamiya2,5, Shinnosuke Uno1, Keitaro Umezawa1, Toshitada Yoshihara3, Seiji Tobita3, Yasuteru Urano1,2,4 (1Grad.
Sch. of Pharm. Sci., The Univ. of Tokyo, 2Grad. Sch. of Med., The Univ. of Tokyo, 3Grad. Sch. Sci. Tech., Gunma Univ., 4AMED CREST, 5JST
PRESTO)2Pos298* 自由行動マウスの脳活動計測を可能にする化学発光膜電位センサーの開発
Development of a chemiluminescent voltage indicator applicable to brain activity recording in freely moving mice
Shigenori Inagaki1, Masakazu Agetsuma2, Hidekazu Tsutsui3,4, Yoshiyuki Arai2, Kazushi Suzuki5, Yuka Jinno4, Yasushi Okamura1,4, Tomoki

2Pos299

2Pos300

Matsuda2, Takeharu Nagai1,2,5 (1FBS., Univ. Osaka, 2ISIR., Univ. Osaka, 3Sch. Mat. Sci., JAIST, 4Grad. Sch. Med., Univ. Osaka, 5Dep of Biotech,
Univ. Osaka)
自動 1 分子イメージング装置の開発
Development of automatic single molecular imaging system
Masato Yasui1, Jun Kozuka1, Michio Hiroshima1, Taku Tsuzuki2, Yasushi Sao3 (1RIKEN QBiC, 2Osaka University, 3RIKEN Cellular Informatics
Laboratory)
Fluorescence recovery after photobleaching (FRAP) analysis of INO80 chromatin remodeling complex
Tsubasa Isogaki1, Yuma Ito1, Shota Ichikawa1, Hiroshi Kimura2, Masahiko Harata3, Kumiko Sakata-Sogawa1, Makio Tokunaga1 (1Sch. Life Sci.

Tech., Tokyo Inst. Tech., 2Inst. Innov. Res., Tokyo Inst. Tech., 3Grad. Agr. Sci., Tohoku Univ.)
2Pos301* The Correspondence between Raman Microspectroscopy and Omics Data
Koseki Kobayashi-Kirschvink1, Hidenori Nakaoka1, Arisa Oda2, Kunihiro Ohta2,3, Yuichi Wakamoto1 (1Dep. Bas. Sci., Univ. Tokyo, 2Dep. Life.
2Pos3022Pos303

Sci., Univ. Tokyo, 3Dep. Bio. Sci., Univ. Tokyo)
電子顕微鏡法のための画像処理パッケージ Eos/PIONE の更なる発展
Further progress of Eos/PIONE for image analysis packages for electron microscopy
Takuo Yasunaga, Takafumi Tsukamoto, Ayaka Iwasaki (Dept. of Biosci. Bioinfo., School of Comp. Sci. Systems, Eng., Kyushu Inst. Tech.)
X 線自由電子レーザーを用いたコヒーレント回折イメージング実験におけるデータ解析の自動化と酵母細胞核の三次元構造解析への応用
Automated data analyses for 3D structural reconstruction of yeast nuclei in coherent diffraction imaging using X-ray free-electron laser
Yuki Sekiguchi1,2, Amane Kobayashi1,2, Koji Okajima1,2, Tomotaka Oroguchi1,2, Masayoshi Nakasako1,2, Masaki Yamamoto2 (1Grad. Sci. Tech.,
Keio Univ., 2RIKEN SPring-8 Center)バイオエンジニアリング / Bioengineering
2Pos304

2Pos305
2Pos306

2Pos307

フォトクロミック分子を利用した低分子量 G タンパク質の光可逆的制御
Photo-regulation of Small G protein RhoA using Photochromic Molecules
Kaori Masuhara1, Masahiro Kuboyama1, Nobuyuki Nishibe2, Shinsaku Maruta1,2 (1Grad. Sch. Bioinfo., Univ. SOKA, 2Dept. Bioinfo., Fac.
Engineer., Univ. SOKA)
Structure and mechanism of the multimerization of small GTPase protein Ras induced by chamical modification at HVR domain
Takashi Hashimoto1, Shinsaku Maruta1, Yasunobu Sugimoto2 (1Soka University, 2Nagoya University)
環状型サイトカインの安定性を向上させる結合末端ループ長の選択
Selection of the loop length about circularized cytokines
Risa Shibuya1, Takamitsu Miyafusa2, Wataru Nishima2, Shinya Honda2 (1Front. Sci. , Univ. of Tokyo, 2BMRI, AIST)
Interaction between ring or linear DNA vs. nanopore/nanoslit
Takayuki Nakayama1, Yoshiaki Iitsuka1, Seiya Minato1, Surat Wangwarunyoo2, Naoto Sakashita1, Kentaro Ishida1, Toshiyuki Mitsui1 (1Coll. of
Sci. & Eng., Aoyama Gakuin Univ., 2Chulalongkorn Univ.)– S119 –2Pos308
2Pos309

Photo-regulation of Small G-proteins Ras Using Photochromic Molecules
Masahiro Kuboyama, Kaori Masuhara, Shinsaku Maruta (Soka University)
脂質膜上チャネル形成のための 10 ナノメートルスケールのポアを持つ DNA オリガミナノ構造
DNA origami nanostruture with pore of ten nanometer scale for forming channel on lipid membrane
Koichiro Katayama1, Ibuki Kawamata1, Yuki Suzuki1,2, Satoshi Murata1, Shin-ichiro Nomura1 (1Graduate school of Engineering, Tohoku

University, 2Frontier Research Institute for Interdisciplinary Sciences, Tohoku University)
2Pos310* 撹拌操作が引き起こすゲノム DNA の二本鎖切断：新規実験手法の提案
How to keep genome-sized DNA safe against stirring stress: Quantitative analysis through single DNA observation
Hayato Kikuchi, Yuko Yoshikawa, Rinko Kubota, Kenichi Yoshikawa (Lab. Biol. Phys., Facul. Life Med. Sci., Doshisha Univ.)
2Pos311* Four-way junction DNA 形成による癌特異的 microRNA 発現パターンの自律的検出
Programmable system for recognition of microRNA expression pattern using four-way junction DNA formation
Moe Hiratani, Masayuki Ohara, Ryuji Kawano (The Dep. of Biotech. and Life Sci., Tokyo Univ. of Agr. and Tech.)2Pos312 光による DNA ハイドロゲルのパターン形成
Patterning of DNA hydrogel using light
Suguru Shimomura, Takahiro Nishimura, Yusuke Ogura, Jun Tanida (Grad. Sch. Info. Sci. & Tech., Osaka Univ.)
2Pos313 キメラ受容体によるバクテリアバイオセンサーの特異性改変
Modification of ligand specificity in bacterial biosensor with hybrid chemoreceptors
Hana Satou2, Nao Fujii2, Takashi Sagawa1, Hiroto Tanaka1, Kazuhiro Oiwa1,2, Hiroaki Kojima1 (1Frontier Lab.,KARC,NICT, 2Sch. Sci., Univ.
Hyogo)
2Pos314* 金ナノ粒子のデジタル計数法による標的 DNA の高感度検出
High-sensitivity Homogeneous DNA hybridization assay by Digital Counting of Gold Nanoparticle Dimers
Takaha Mizuguchi, Keiko Esashika, Toshiharu Saiki (Grad. Sch. Sci. Tech., Keio Univ.)
2Pos315* A single integrated gene nano-chip functioning in an artificial cellTakeya Masubuchi1, Masayuki Endo2, Ryo Iizuka3, Ayaka Iguchi4, Yoon Doung Hyun4, Tetsushi Sekiguchi5, Hao Qi1,6, Ryosuke Iinuma1, Yuya
Miyazono1, Shuichi Shoji4, Takashi Funatsu3, Hiroshi Sugiyama2,7, Yoshie Harada2, Takuya Ueda1, Hisashi Tadakuma1,2 (1Grad. Sch. of FrontierSci., The Univ. of Tokyo, 2iCeMS, Kyoto Univ., 3Grad. Sch. of Pharm. Sci., The Univ. of Tokyo, 4Grad. Sch. of Adv. Sci. and Eng., Waseda Univ.,
5Research Organization for Nano & Life Innovation, Waseda Univ., 6Dept. of Chem. Eng. and Tech., Tianjin Univ., 7Grad. Sch. of Sci., Kyoto Univ.)

2Pos316* アメーバ型分子ロボット：モータータンパク質と DNA デバイスを内包した巨大リポソームの形状変化とその制御
Amoeba type molecular robot: controlling shape change of giant liposome entrapping molecular motors and DNA circuits
2Pos317

Yusuke Sato1, Yuichi Hiratsuka2, Ibuki Kawamata1, Satoshi Murata1, Shin-ichiro Nomura1 (1Grad. Sho. Eng., Tohoku Univ., 2Sch. Mat. Sci., JAIST)
心筋細胞集団同士を繋ぐ線維芽細胞の距離に対する同期
Synchronization of large clusters of cardiomyocytes connected with fibroblasts and its distance change

2Pos318

Shota Miyakoshi1, Toshiyuki Mitsui2, Tomoyuki Kaneko1 (1LaRC, Grad. Sci. Eng., Hosei Univ., 2Dept. Math. Phys. Col. Sci. Eng,. Aoyama Univ.)
目的細胞の回収を目指した微小液滴内培養法の開発
Development of a single cell cultivating method using a microdroplets forming technique for sorting specific cells
Hideyuki Terazono1, Masao Odaka1, Akihiro Hattori1, Kenji Matsuura1, Kenji Yasuda1,2 (1WASEDA Biosci. Res. Inst. Singapore (WABIOS),
Waseda Univ., 2Dept. Physics, Waseda Univ.)

第3 日目（11 月27 日（日））／Day 3（Nov. 27 Sun.）大会議室 101 ＋102、多目的ホール／Conference Room 101+102, Multi-Purpose Hall

蛋白質：構造 / Protein: Structure
3Pos001

Single Particle Analysis of EhV-ATPase by Phase-contrast cryo-Electron Microscopy
Jun Tsunoda1,2, Chihong Song2, Fabiana Lica Yakushiji3, Takeshi Murata3, Hiroshi Ueno4, Junichi Takagi6, Ryota Iino1,5, Kazuyoshi Murata1,2

3Pos002(1SOKENDAI, 2NIPS, 3Dept. Chem., Chiba Univ., 4Dept. Appl. Chem., Sch. Eng., Univ. Tokyo, 5OIIB/IMS, 6Osaka Univ. IPR)TEM と ASEM を用いたタンパク質複合体・細胞組織の親水環境での観察Electromicroscopy of protein complexes, cells and tissues in hydrophilic environment

3Pos003

Chikara Sato1, Nassirhadjy Memtily1, Mari Sato1, Toshiko Yamazawa2, Masaaki Kawata1 (1AIST, 2Dept. Mol Physiol, Jikei Univ. Sch. Med.)
クライオ電子顕微鏡による Thermus thermophilus V-ATPase の単粒子解析
Single-particle analysis of Thermus thermophilus V-ATPase by Cryo-EM

3Pos004

Atsuko Nakanishi1, Jun-ichi Kishikawa1, Kaoru Mitsuoka2, Ken Yokoyama1 (1Dept. LifeSci. Kyoto Sangyo Univ., 2Res. Ctr. UVHEM. Univ.
Osaka.)
クライオ電子顕微鏡単粒子解析によるマウスノロウイルス VLP の構造解析
Structural Analyses of Murine Norovirus VLPs by Cryo-Electron Microscopy Single Particle Analysis
Chihong Song1, Motohiro Miki2,3, Reiko Todaka2, Kei Haga2, Akira Fujimoto2, Kazuhiko Katayama2, Kazuyoshi Murata1 (1NIPS, 2NIID, 3Denka
Seiken)– S120 –3Pos005

2D ハイブリッド解析による電子顕微鏡平均画像の成分解析
Component analysis of averaged EM images by 2D hybrid analysis
Atsushi Matsumoto1, Junichi Takagi2, Atsushi Kawaguchi3, Kenji Iwasaki2 (1National Institutes for Quantum and Radiological Science and3Pos006

3Pos007

Technology, 2Osaka University, 3Tsukuba University)
Structural analysis for V1-ATPase from a variety of prokaryotes
Nao Takeuchi1, Atsuko Nakanishi1, Jun-ichi Kishikawa1, Kaoru Mitsuoka2, Ken Yokoyama1 (1Kyoto Sangyo Univ. LifeSci., 2Osaka Univ. Res. Ctr.
UHVEM)
最新低温電子顕微鏡“CryoARM”の性能
Performance of State-of-the-art CryoEM, named “CryoARM”
Takayuki Kato1, Naoki Hosogi2, Takeshi Kaneko2, Isamu Ishikawa2, Keiichi Namba1,3 (1Grad. Sch. of Front. Biosci., Osaka Univ., 2JEOL,
3RIKEN, QBiC)

3Pos008

3Pos009

電顕３次元密度マップから α へリックスを認識する混合正規分布モデルの開発
Detection of alpha-helices from the 3D EM density map using Gaussian mixture modelTakeshi Kawabata, Haruki Nakamura (IPR, Osaka U.)
Towards Understanding the Molecular Architecture of Human DNA Polymerase δ using Electron Microscopy and Computational
Modeling
Ashutosh Srivastava1, Yuji Masuda2, Jiro Usukura3, Motoshi Suzuki4, Florence Tama1,5 (1ITbM, Nagoya Univ., 2Res. Inst. Env. Med., Nagoya

3Pos010

3Pos011

Univ., 3Str. Bio. Cen., Grad. Sch. Sci., Nagoya Univ., 4Div. Mol. Carc., Grad. Sch. Med., Nagoya Univ., 5Dept. Phys., Sch. Sci., Nagoya Univ.)
GPI アタッチメントシグナル領域の二次構造解析
Secondary structural analysis of GPI attachment regions
Keiya Inoue1, Daiki Takahashi2, Tatsuki Kikegawa2, Kenji Etchuya2, Yuri Mukai1,2 (1Sch. Sci. & Tech., Meiji Univ., 2Grad. Sch. Sci. & Tech., Meiji
Univ.)
インターセクチン２のコンホメーション解析
Conformational Analysis of Multidomein Protein Intersectin 2
Kazutaka Murayama1,2, Miyuki Murayama-Kato2, Ryogo Akasaka2, Daisuke Sugimori3, Mikako Shirouzu2 (1Tohoku Univ. Biomed. Eng.,
2RIKEN, CLST, 3Fukushima Univ. Symbio. Sys. Sci.)

3Pos012

3Pos013

3Pos014タンパク質の構造変性と回転拡散係数
Rotational diffusion coefficients of proteins along denaturation curve
Yoshitake Tomoyuki, Terazima Masahide (Graduate School of Science, Kyoto University)
シアノバクテリア時計タンパク質 KaiC の AFM 観察
AFM observation of a ring-shaped structure of KaiC
Jun Abe, Atsushi Mukaiyama, Yoshihiko Furuike, Shuji Akiyama (Division of Trans-Hierarchical Molecular Systems, Research Center ofIntegrative Molecular Systems (CIMoS), Institute for Molecular Science (IMS))
高速 AFM による 20S プロテアソーム関連タンパク質の動態観察
Dynamics observation of the 20S proteasome-related proteins using High-Speed AFM
Toshiya Kozai1, Tadashi Satoh2, Arunima Sikdar3,4, Hirokazu Yagi2, Maho Yagi-Utsumi3, Takayuki Uchihashi1, Toshio Ando1, Koichi Kato3,4

3Pos015

3Pos016

3Pos017

3Pos018
3Pos019

3Pos020

3Pos021
3Pos022(1Dept. of phys., Kanazawa Univ., 2Grad. Sch. Pharm. Sci., Nagoya City Univ., 3Okazaki Inst. Integ. Biosci., 4Nat. Univ. SOKENDAI)
立体構造予測において疎水効果を評価するための新しい指標： 仮想原子の周りのコンタクト数
A new measure for hydrophobicity: Contact number around an imaginary atom
Yota Masuyama, George Chikenji (Grad. Sch. Eng., Nagoya Univ.)
統合失調症疾患感受性遺伝子産物 G72 タンパク質の構造機能予測
Structure and function prediction of the G72 protein, the product of a susceptible gene for schizophrenia
Yusuke Kato, Kiyoshi Fukui (Institute for Enzyme Research, Tokushima University)
残基間平均距離統計に基づくコンタクトマップによる天然変性領域の予測
Prediction of IDRs by a contact map based on inter residue average distance statistics
Takumi Shimomura, Takeshi Kikuchi (Univ. Ritsumei)
A new threading method based on the physical characteristics of sequence-structure compatibility
Kyosuke Tomoda, Yota Masuyama, George Chikenji (Grad. Sch. Eng., Nagoya Univ.)
EMDB, PDB, SASBDB 中の多階層構造データを対象としたウェブベースのサービス
Web based services for multiscale structure data in EMDB, PDB and SASBDB
Hirofumi Suzuki1,2, Takeshi Kawabata1, Gert-Jan Bekker1,2,3, Haruki Nakamura1,2 (1IPR, Osaka Univ., 2PDBj, 3FBS, Osaka Univ.)
単独で構造を維持するドメインデータベース「IS-Dom」の他のデータベースに依存しない拡張
Standalone definition of putatively independent structural domain: IS-Dom
Soichiro Ide1, Teppei Ebina2, Richa Tanbi1, Yutaka Kuroda1 (1Tokyo University of Agriculture and Technology, 2Department of Physiology,
Graduate School of Medicine, The University of Tokyo)
Attempts at CA-type formal analysis of fibrous assembly of particles
Takashi Konno (Mol. Physiol., Med., Univ. Fukui)
生物の低温適応と蛋白質配列の進化
Cold adaptation of organisms and the evolution of protein sequences
Matsuyuki Shirota1,2,3 (1Grad. Sch. Med., Tohoku Univ., 2ToMMo, Tohoku Univ., 3Grad. Sch. Inform Sci., Tohoku Univ.)– S121 –蛋白質：構造機能相関 / Protein: Structure & Function
3Pos023

3Pos024

スライディングとストランド間移動を用いたヒト抗ウイルス因子 APOBEC3G の高効率な DNA 配列探索：実時間 NMR による新知見
Sliding and intersegmental transfer on DNA enhance target search of human anti-viral factor APOBEC3G: insight by the real-time NMR
study
Keisuke Kamba1, Takashi Nagata1,2, Masato Katahira1,2 (1Inst. of Adv. Energy, Kyoto Univ., 2Grad. Sch. of Energy Sci., Kyoto Univ.)
分子動力学ドッキング・シミュレーションによるスーパーコイル DNA 結合（SDR）ペプチドとクロスオーバー DNA の選択的結合メ
カニズムの解析
Molecular dynamics docking study on selective binding mechanisms of supercoiled-DNA recognition (SDR) peptide and spatially-crossover
DNA
Hiroshi Nishigami1, Kakeru Sakabe1, Jiyoung Kang1, Kuniaki Sano2, Kimiko Tsutsui2, Ken Tsutsui2, Kazuhiko Yamasaki3, Masaru Tateno1

3Pos025
3Pos026(1Grad. Sch. Life Sci., Univ. Hyogo, 2Dept. Neurogenomics, Grad. Sch. Med. Dent. Pharm. Sci., Okayama Univ., 3Biomedical Res. Inst., AIST)
Unravelling the mechanism of (6-4) photolyase enzyme
Hisham Dokainish, AKio Kitao (The University of Tokyo)
暗視野顕微鏡を用いたタンパク質過飽和溶液中ナノスケールダイナミクス構造観察
Nano-scale Observations of Supersaturated Protein Dynamics using Dark-Field Microscopy
Kazuki Yoshimura1, Yufuku Matsushita1, Keigo Ikezaki1, Hiroshi Sekiguchi2, Yuji Goto3, Yuji Sasaki1,2 (1Grad. Sch. Front. Sci., Univ. Tokyo,
2JASRI/SPring-8, 3IPR, Osaka Univ.)

3Pos027

Analysis and control of protein crystallization using short peptide tags without affecting structure, thermal stability and function

3Pos028

MM. Islam1, N. Shigeyoshi2, K. Noguchi3, M. Yohda3, SI. Kidokoro2, Y Kuroda3 (1CU, 2NUT, 3TUAT)
滴定 X 線溶液散乱測定を用いたアダプター蛋白質 GGA-ユビキチン相互作用の解析
An analysis of the interaction of GGA with ubiquitin by using titration SAXS measurement
Yugo Hayashi1, Miho Shinohara1, Keito Yoshida1, Yoichi Yamazaki1, Kazuhisa Nakayama2, Soichi Wakatsuki3, Hironari Kamikubo1 (1Grad. Sch.

3Pos029

3Pos030

3Pos031

3Pos032

Mat. Sci., NAIST, 2Grad. Sch. Pharm., Kyoto Univ., 3Stanford Univ.)
K63 ジユビキチンと TAB2 複合体の拡張サンプリング
Large-scale configurational sampling of K63-linked di-ubiquitin complexed with TAB2
Keiichi Inariyama1, Hafumi Nishi2, Kei Moritsugu1, Akinori Kidera1 (1Grad. Sch. of Med. Life Sci., Yokohama City University, 2Grad. Sch. Info.
Sci., Tohoku University)
Periodic Formation of the Cyanobacterial Circadian Clock Protein Complexes
Shun Terauchi1, Takahiro Iida1,2, Kentaro Ishii2, Masahiro Ishiura2, Kosuke Maki1 (1Sch. of Sci., Nagoya Univ., 2Center for Gene Res., Nagoya
Univ.)
広角溶液散乱測定のための環境整備と時計タンパク質への応用
Wide-angle x-ray scattering studies on circadian clock systems
Shuji Akiyama1,2, Takaaki Hikima2, Atsushi Mukaiyama1,2, Jun Abe1,2, Yoshihiko Furuike1,2 (1CIMoS, IMS, 2RIKEN SPring-8 Center)
X 線小角散乱法を用いた神経軸索伸長系関連蛋白質 shootin1 の動的な構造変化の解析
Structural alteration of shootin1 upon phosphorylation revealed by using small angle x-ray scattering
Shoki Nakata1, Keito Yoshida1, Kentarou Baba2, Yohei Shibata1, Yoichi Yamazaki1, Naoyuki Inagaki2, Hironari Kamikubo1 (1Grad. Sch. of Mater.

3Pos034

Sci., Nara Inst. of Sci. & Tech., 2Grad. Sch. of Biol. Sci., Nara Inst. of Sci. & Tech.)
Dynamical system of alpha-crystallin oligomers
Rintaro Inoue, Takumi Takata, Noriko Fujii, Masaaki Sugiyama, Nobuhiro Sato, Yojiro Oba (Research Reactor Institute,Kyoto University)
Refinement of Cryo-EM Structures Using Scattering Factors of Charged Atoms

3Pos035

Koji Yonekura, Saori Maki-Yonekura, Rei Matsuoka, Yoshiki Yamashita, Fumie Iwabuki, Maiko Tanaka (RIKEN SPring-8 Center)
Visualization of 11- and 34-fold rotational symmetries in the MS ring of the bacterial flagellum by electron cryomicroscopy

3Pos033

Akihiro Kawamoto1, Ayana Kaido2, Miki Kinoshita1, Tomoko Miyata1, Tohru Minamino1, Takayuki Kato1, Keiichi Namba1,3 (1Grad. Sch.
3Pos036

3Pos037
3Pos038
3Pos039

3Pos040

Frontier Biosci., Osaka Univ., 2Dept. Food Science and Nutrition., Doshisha Women's College of Liberal Arts, 3QBiC, RIKEN)
単粒子コヒーレント X 線回折像の類似積判定のためのマルチステップアルゴリズム
Multistep similarity detection algorithm for single particle X-ray coherent diffractions
Atsushi Tokuhisa1, Osamu Miyashita1, Florence Tama1,2 (1AICS, RIKEN, 2Department of Physics, Nagoya University)
Molecular determinants of the ATP binding properties of the ε subunit from bacterial ATP synthases
Alexander Krah1,2, Yasuyuki Kato-Yamada3, Changbong Hyeon2, Shoji Takada1 (1Dept. Biophys., Kyoto Uni., 2KIAS, 3Dept. Life Sci., Rikkyo Uni.)
Microtubule stability and the tubulin molecule interactions within the microtubule lattice
Kenta Hirasada, Daisuke Yamamoto, Miho Katsuki (Fukuoka Univ., Faculty of Science)
隣り合うチューブリン存在下と非存在下における α/β チューブリン C 末端の異なる三次元空間分布
Three-dimensional distributions of α/β-tubulin C-terminal tails and the influence of neighboring tubulins
Koji Umezawa1,2, Yukinobu Mizuhara3, Jun Ohnuki3, Mitsunori Takano3 (1Grad. Sch. of Sci. & Tech., Shinshu Univ., 2IBS, Shinshu Univ., 3Grad.
Scl. of Adv. Sci. & Eng., Waseda Univ.)
F アクチンの水和状態は Mg2+/Ca2+イオンに強く依存する
Strong Mg/Ca Ion Dependence of Hydration State of F-actin
Makoto Suzuki1, Asato Imao1, George Mogami1, Ryotaro Chishima1, Takahiro Watanabe1, Takaya Yamaguchi1, Nobuyuki Morimoto1, Tetsuichi
Wazawa2 (1Grad. School of Eng. Tohoku Univ., 2The Institute of Scientific and Industrial Research, Osaka University)– S122 –3Pos041

3Pos042

3Pos043

アクチンフィラメントに結合したローダミンファロイジン蛍光のゆらぎ
Fluctuation of rhodamine-phalloidin fluorescence along actin filaments
Taro Ueda1,3, Saku Kijima2,3, Takahiro Suzuki1 (1Dept. of Physics, Waseda Univ., 2Biomed. Res. Inst., AIST, 3Grad. Sch. Life Environ. Sci., Univ.
of Tsukuba)
OH 伸縮振動のラマン分光によるミオシン S1 および他のいくつかのタンパク質の水和状態の測定
Hydration study on myosin subfragment-1 (S1) and some other proteins by Raman OH-atretching spectroscopy
Yuki Ochiai1, George Mogami1, Tetsuo Taniuchi2, Makoto Suzuki1 (1Grad. Sch. Eng., Univ. Tohoku, 2IMRAM, Univ. Tohoku)
ウルトラファインバブル水中のタンパク質構造の研究
Study of protein structure in ultra-fine bubble water
Mitsuhiro Hirai1, Satoshi Ajito1, Kosuke Takahashi1, Noboru Ohta2, Tatsuo Iwasa3 (1Grad. Sch. Sci. Tech., Gunma Univ., 2Jpn. Syn. Rad. Res.

3Pos044

3Pos045

3Pos046

3Pos047

Inst., 3Muroran Inst. Tech.)
3D−RISM 理論を応用した溶液中における Met−enkephalin の構造揺らぎの解析
Analysis of structural fluctuations of Met-encephalin in the solution phase by means of 3D-RISM theory
Masatake Sugita1, Fumio Hirata2 (1Dept. of Bioinfo., Col. of Life Sci., Ritsumeikan Univ., 2Toyota Phys. & Chem. Res. Inst.)
分子動力学シミュレーションとエネルギー表示理論を用いた共溶媒変性効果の自由エネルギー解析
Free energy analysis of cosolvent effect through molecular dynamics simulation and energy-representation method
Yu Yamamori, Nobuyuki Matsubayasi (Grad. Sch. of Eng. Sci., Osaka Univ.)
タンパク分子内情報伝達を実現する構造基盤の探索―既知の構造から
Search for Common Structural Basis of Mechanical Communication in Proteins: from Known Structures
Yuichi Togashi (RcMcD, Hiroshima Univ.)
CAPAXIS と PyMOL を用いたウイルス粒子脱殻の描画・操作
Modeling of uncoating of virus capsid by using CAPAXIS and PyMOL
Shunsuke Sato1, Aya Kosugi1, Go Wabanabe2, Shigetaka Yoneda2 (1Grad. Sch. Sci., Kitasato Univ., 2Sch. Sci., Kitasato Univ.)

蛋白質：物性 / Protein: Property
3Pos048

生体分子の分子動力学計算を取り扱う高速な QM/MM 理論の開発
Development of rapid QM/MM approach for biomolecular simulations
Hiroaki Nishizawa1, Hisashi Okumura1,2 (1IMS, 2Sokendai)

3Pos049

3Pos050

3Pos051

3Pos052

タンパク質の基準振動モードのネットワーク解析：中心性指標の計算
Network analysis of normal modes of proteins: calculations of various centrality measures
Hiroshi Wako1, Shigeru Endo2 (1Sch. of Soc. Sci., Waseda Univ., 2Sch. of Sci., Kitasato Univ.)
タンパク質構造変化における経路の多様性：分子動力学シミュレーションによる解析
A variety of pathways for a conformational change of a protein investigated by molecular dynamics simulation
Sotaro Fuchigami (Grad. Sch. of Medical Life Science, Yokohama City Univ.)
カメレオンモデルによる NtrC の構造転移機構の研究
Mechanism of conformational transition of NtrC studied by using chameleon model
Shinya Abe1, Atsushi Mizuno2, Masaki Sasai1, Tomoki P. Terada1 (1Dept. Comput. Sci. Eng., Grad. Sch. Eng., Nagoya Univ., 2Dept. Appl. Phys.,Sch. Eng., Nagoya Univ.)
Oct4 の 2 つの DNA 結合サブドメインを結ぶ柔軟な linker 領域の自由エネルギー地形
Free-energy landscape of the flexible linker connecting two DNA-binding subdomains of Oct4
Tomonori Hayami1,2, Shoji Takada3, Haruki Nakamura1, Junichi Higo1 (1Inst. Protein Res., Osaka Univ., 2Grad. Sch. Frontier Biosci., Osaka

3Pos053

3Pos054

3Pos055

Univ., 3Dept. Biophys., Grad. Sch. Sci., Kyoto Univ.)
タンパク質の協同的な折れたたみとループのつながり方の関係
Relation between cooperative protein folding and loop connections
Nobu C. Shirai1, Shintaro Minami2 (1Center for Information Technologies and Networks, Mie University, 2Graduate School of Information Science,
Nagoya University)
酸曝露後中和による抗体のフォールディングと凝集: 二種の光子相関分光法による追跡
Folding versus aggregation of an antibody initiated by pH-shift stress: Double tracking by photon correlation spectroscopies
Hiroshi Imamura, Akira Sasaki, Shinya Honda (Natl Inst Adv Indust Sci. Tech.)
NMR を用いた血清環境での相互作用解析
NMR approach for understanding protein interactions in serum environments
Saeko Yanaka1,2, Rina Yogo1,2, Hirokazu Yagi1,2, Koichi Kato1,2 (1Department of Bioorganization Research, Okazaki Institute for Integrative

3Pos056

Bioscience and Institute for Molecular Science, National Institutes of Natural Sciences, 2Department of Structural Biology and BiomolecularEngineering, Graduate School of Pharmaceutical Sciences, Nagoya City University)
酸化と酵素切断が LDL の物性に与える影響
Physical properties of low-density lipoprotein after oxidation or proteolytic enzyme treatment
Seiji Takeda1, Agus Subagyo2, Shu-Ping Hui1, Hirotoshi Fuda1, Kazuhisa Sueoka2, Hitoshi Chiba1 (1Fac. Health Sci., Univ. Hokkaido, 2Grad. Sch.
Inf. Sci. Tech., Univ. Hokkaido)– S123 –3Pos057

3Pos058

イムノグロブリン G のマルチドメイン構造形成におけるエントロピー効果
Entropic stabilization of the multi-domain architecture in immunoglobulin G
Seiki Yageta, Hiroshi Imamura, Shinya Honda (Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology)
統計熱力学に基づいたサーモフィリックロドプシンの熱安定化変異体の作製
Identification of thermostabilizing mutations for thermophilic rhodopsin based on statistical thermodynamics
Sayaka Nemoto1, Satoshi Yasuda1,2, Kenji Mizutani1, Takashi Tsukamoto3, Yuki Sudo3, Masahiro Kinoshita2, Takeshi Murata1,4 (1Grad. Sch. Sci.,

3Pos059

Univ. Chiba, 2Inst. Advanced Energy, Univ. Kyoto, 3Grad. Sch. Medicine, Dentistry, and Pharmaceutical Sciences, 4JST, PRESTO)
Amorphous aggregation of cytochrome c with inherently low amyloidogenicity is characterized by the phase diagram
Yuxi Lin1, Jozsef Kardos2, Misaki Kinoshita1, Toshihiko Sugiki1, Koichiro Ishimori3, Yuji Goto1, Young-Ho Lee1 (1Institute for Protein Research,

3Pos060

Osaka University, 2Department of Biochemistry, Eotvos Lorand University, 3Department of Chemistry, Faculty of Science, Hokkaido University)
The virial coefficients based on the rotaitional diffusion as a criterion of the protein crystallizaton

3Pos061

Yudai Katsuki1, Akane Kato2, Etsuko Nishimoto3 (1sch. Agr., Kyushu Univ., 2Grad. Sch. Bioresour. Bioenviron. Sci., Kyushu Univ., 3Fac. Agr.,
Kyushu Univ.)
The hydration state near the binding site of human Serum Albumin revealed by the time-resolved fluorescence spectrum of Trp214

3Pos062

3Pos063

Shoutaro Kubo1, Etsuko Nishimoto2 (1Sch. Agr., Kyushu Univ., 2Fac. Agr., Kyushu Univ.)
アクチンフィラメントの圧電特性 III
Piezoelectric property of an actin filament III
Jun Ohnuki, Takato Sato, Hideyo Okamura, Taro Q.P. Uyeda, Mitsunori Takano (Dept. of Pure & Appl. Phys., Waseda Univ.)
テトラヒメナ外腕ダイニン重鎖（Dyh3p)における運動系の開発と運動特性
Motor domain-based motility system and motile properties of alpha heavy chain in Tetrahymena outer arm dynein
Masaki Edamatsu (Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo)

蛋白質：計測･解析 / Protein: Measurement & Analysis
3Pos064

タンパク質の NMR 解析が困難な系にも有効なシグナル帰属法
Signal assignment strategy for protein NMR under challenging conditions
Takuma Kasai1,2, Kae Higuchi1, Kohsuke Inomata1,3, Takanori Kigawa1,2,4 (1RIKEN QBiC, 2CREST, JST, 3PRESTO, JST, 4Sch. Comput., Tokyo

3Pos065

Inst. of Tech.)
二量子遷移 EPR 距離測定における短距離成分の影響
Effects of Short Distance Components on Double Quantum Coherence EPR Distance Measurements

3Pos066

Yasunori Ohba1, Syouji Ueki2, Toshiaki Arata3 (1IMRAM、Tohoku Univ., 2Fac. Pharm. Sci., Tokushima Bunri Univ., 3Dep. Biol. Sci., Osaka Univ.)
天然変性タンパク質の SAXS プロフィル評価法を開発するための新たな枠組み
A novel framework for developing the evaluation method of SAXS profile of IDP

3Pos067

Yasutaka Seki1, Shigeyoshi Nakamura2 (1Kochi Med. Sch., 2Kitakushu Nat. Coll. of Tech.)
SAXS とアミノ酸残基レベル二次構造情報からのタンパク質立体構造の構築
Protein structure constructed with SAXS and secondary structures at amino acid residue level
Yasumasa Morimoto1, Takayuki Ichioka1, Toru Terada2, Kentaro Shimizu2, Yoshitaka Matsumura1, Masaki Kojima1 (1Sch. of Life Sci., Tokyo

3Pos068

3Pos069

3Pos070

Univ. of Pharm. and Life Sci., 2Dept. of Appl. Biol. Chem., Grad. Sch. of Agr. and Life Sci., The Univ. of Tokyo)
タンパク質複合体の解離過程の分子動力学
A Steered Molecular Dynamics to Understand the Dissociation Process of Protein Complex
Yutaka Ueno, Yuki Mochizuki (AIST Kansai, Biomedical Research)
脂質ナノディスクと ZMW 法を用いた高濃度リガンドでの計測が可能な膜タンパク 1 分子計測系の構築
Nanodiscs platform on ZMWs for single-molecule imaging of membrane proteins at high ligand concentration
Keisuke Tsukada, Kazushi Isomura, Tomotaka Komori, Sotaro Uemura (Dep. Bio. Sci., Grad. Sch. Sci., Univ. Tokyo)
X 線 1 分子追跡法による TRPV1 チャネルの分子運動解析
3D Motion Maps of TRPV1 cation channel depicted by Diffracted X-Ray Tracking Method
Kazuhiro Mio1, Keigo Ikezaki2, Hiroshi Sekiguchi3, Yuhuku Matsushita2, Tai Kubo1, Yuji C. Sasaki2 (1Molprof, AIST, 2Frontier Science, Adv.

3Pos071

3Pos072

3Pos073

Material Sci., Univ. of Tokyo, 3JASRI)
細胞性粘菌の生きた細胞での膜タンパク質の拡散の網羅的解析
Comprehensive Diffusion Analysis of Membrane Proteins in Living Dictyostelium Cells
Kazutoshi Takebayashi1, Yukihiro Miyanaga2, Masahiro Ueda2,3 (1Grad. Sch. Sci., Univ. Osaka, 2Grad. Sch. FBS., Univ. Osaka, 3QBiC., Riken)
細菌Ⅲ型分泌装置の回転運動によるエフェクター輸送の制御
Rotation of needle-like type III secretion apparatus directly regulates its effector transport
Takashi Ohgita, Kohei Fukuda, Kyoko Momiyama, Naoki Hayashi, Naomasa Gotoh, Hiroyuki Saito (Kyoto Pharm. Univ.)
抗体修飾ナノニードルと AFM を用いた引っ張り試験による細胞骨格の機械的特性の解析
Analysis of mechanical property of cytoskeleton by tensile test for intermediate filament using antibody-modified nanoneedle and AFM
Moe Susaki1, Itaru Takeda2, Ayana Yamagishi3, Yuta Takano2, Tomoko Okada3, Chikashi Nakamura1,2,3 (1Fac. Eng., Tokyo Univ. Agric. Technol.,
2Grad. Sch. Eng., Tokyo Univ. Agric. Technol., 3Biomed. Res. Inst., AIST)

3Pos074

テンダム遺伝子を用いた無細胞タンパク質発現ノイズの解析
Stochastic gene expression in cell-free system by tandem genes
Shiori Fujimoto, Yi Zhang, Kazuhito Tabata, Hiroyuki Noji (Grad. Sch. Eng., Univ. Tokyo)– S124 –ヘム蛋白質 / Heme proteins
3Pos075

凍結トラップ結晶構造解析と時間分解分光を用いた P450nor の反応中間体の解析
Reaction Intermediate Analysis of P450nor Using Freeze-Trap X-ray Crystallography and Time-Resolved Spectroscopy
Takashi Nomura1, Takuma Nishida2, Takehiko Tosha1, Hiroshi Sugimoto1, Yoshitsugu Shiro1,2, Minoru Kubo1,3 (1Harima Inst., Riken, 2Grad. Sch.

3Pos076

Sci., Univ. Hyogo, 3JST PRESTO)
結晶状態ヘモグロビンの大規模四次構造変化の直接観測
Direct observation of large-scale quaternary motions of hemoglobin in a crystalline state

3Pos077

Naoya Shibayama1, Mio Ohki2, Sam-Yong Park2 (1Jichi Med. Univ., Div. of Biophys., 2Yokohama City Univ., Drug Design Lab.)
構造状態と関係したヘモグロビンのピコ秒ダイナミクスの変化
Changes in the picosecond dynamics of hemoglobin related to the structural states
Satoru Fujiwara1, Toshiyuki Chatake2, Tatsuhito Matsuo1, Fumiaki Kono1, Taiki Tominaga3, Kaoru Shibata4, Ayana Sato5, Naoya Shibayama5

3Pos078(1QuBS, QST, 2RRI, Kyoto Univ., 3CROSS-Tokai, 4J-PARC Center, 5Div. Biophysics, Jichi Med. Univ.)
四量体ヒトヘモグロビンにおける 2 つの α 鎖に特有の Fe-His 結合と四次構造との関連
Distinct Fe-His bond of two α subunits in human α2β2 tetramer hemoglobins and their quaternary structures
Shigenori Nagatomo1, Kazuya Saito1, Masako Nagai2, Takashi Ogura3, Teizo Kitagawa3 (1Dept. Chem., Univ. Tsukuba, 2Res. Center Micro-Nano

3Pos079

Tech., Hosei Univ., 3Grad. Sch. Life Sci., Univ. Hyogo)
神経保護作用を持つヒトニューログロビンとヘテロ三量体 Gi 蛋白質 α サブユニットとの相互作用に重要なアミノ酸残基の特定
Identification of residues crucial for the interaction between human neuroprotective protein “neuroglobin” and Gαi

3Pos080

Nozomu Takahashi, Keisuke Wakasugi (Dep. of Life Sci., Grad. Sch. of Arts and Sci., Univ. of Tokyo)
ドメインスワッピングによるミオグロビン二量体の形成
Formation of myoglobin dimer by domain swapping

3Pos082

Institute of Science and Technology, 2Graduate School of Life Science, University of Hyogo)
共鳴ラマン分光法による 2 価コバラミンの軸配位子に依存した構造変化の検出
Resonance Raman Study of Cobalamin (II): Axial Ligands-Dependent Structural Change

3Pos083

Kaoru Mieda1, Abdullah Al Mamun2, Pawel M. Kozlowski2, Takashi Ogura1 (1Grad. Sch. Sci., Univ. Hyogo, 2Dept. of Chem., Univ. of Louisville)
タンパク質中のヘムの歪みの統計的解析
Statistical analysis of heme distortion in protein

3Pos084

Yasuhiro Imada1, Yusuke Kanematsu2, Hiroko X. Kondo2, Yu Takano2 (1IPR, Osaka Univ., 2Grad. Sch. Info. Sci., Hiroshima City Univ.)
アンサンブルドッキングを用いた CYP1A2 化合物の代謝部位予測
Prediction of site of metabolism of compounds for CYP1A2 by ensemble docking simulation

Satoshi Nagao1, Ayaka Suda1, Hisashi Kobayashi1, Naoki Shibata2, Yoshiki Higuchi2, Shun Hirota1 (1Graduate School of Materials Science, Nara

Hiroaki Saito1, Taku Mizukami2, Yoshinori Hirano1, Takao Otsuka1, Noriaki Okimoto1, Makoto Taiji1 (1RIKEN Quantitative Biology Center(QBiC), 2Japan Institute of Science and Technology (JAIST))

膜蛋白質 / Membrane proteins
3Pos085

3Pos086

本来の構造と機能を保持したウシミトコンドリア呼吸鎖複合体の精製
Purification of native mitochondrial respiratory complexes from bovine heart
Satoru Shimada, Shigefumi Uene, Marika Oosaki, Ryoko Takahashi, Harunobu Shimomura, Kaoru Mieda, Shintaro Maeda, Masahide Hikita,
Kyoko Shinzawa-Itoh (Grad. Sch. Life Sci., Univ. Hyogo)
部分フッ素化リン脂質二分子膜中膜タンパク質バクテリオロドプシンの構造と安定性に対するフッ化アルキル鎖長依存性
Structural stability of bacteriorhodopsin in partially fluorinated analogs of DMPC with different perfluoroalkyl chain lengths
Mami Hashimoto1, Yuka Murai1, Masaru Yoshino1, Toshinori Motegi1, Takashi Kikukawa2, Toshiyuki Takagi3, Hiroshi Takahashi1, Hideki Amii1,

3Pos087

Toshiyuki Kanamori3, Masashi Sonoyama1 (1Div. Mol. Sci., Gunma Univ., 2Fac. Adv. Life Sci., Hokkaido Univ., 3AIST)
高圧下で界面活性剤を用いて昆虫細胞膜から可溶化した PBANR（クラス-A GPCR）はリガンド結合能を保持する
PBANR, a class-A GPCR, solubilized under high hydrostatic pressure retains its ligand binding ability
Yukie Katayama1, Tatsuya Suzuki1, Tatsuki Ebisawa1, Jun Ohtsuka1, Ryo Natsume2, Yu-Hua Lo3, Toshiya Senda3, Toshihiro Nagamine4, J.Joe

3Pos088

Hull5, Shogo Matsumoto4, Hiromichi Nagasawa1,6, Koji Nagata1, Masaru Tanokura1 (1UTokyo, 2TDU, 3KEK-PF, 4RIKEN, 5USDA-ARS, 6ZJU)
GraDeR：単粒子解析等の膜タンパク質資料調整
GraDeR: membrane protein preparation for single particle cryoEM & more

3Pos089

Fujiyoshi3, Holger Stark4 (1Grad. Sch. Sci., Univ. Hyogo, 2Kyoto Sangyo Univ., 3CeSPI, Nagoya Univ., 4Max Planck Society, 5CREST, JST)
エーテル型部分フッ素化リン脂質膜中の膜タンパク質バクテリオロドプシンのサーモクロミズム
Thermochromism of bacteriorhodopsin in partially fluorinated di-o-tetradecylphosphocholine vesicles

Christoph Gerle1,5, Florian Hauer4, Niels Fischer4, Kyoko Shinzawa-Itoh1, Satoru Shimada1,5, Ken Yokoyama2, Atsunori Oshima3, Yoshinori

Masaya Miyazaki1, Naoyuki Tuchida1, Toshinori Motegi1, Takashi Kikukawa2, Toshiyuki Takagi3, Hiroshi Takahashi1, Hideki Amii1, Toshiyuki
3Pos090

Kanamori3, Masashi Sonoyama1 (1Fac. Sci. Tech., Gunma. Univ., 2Grad. Sch. Sci., Hokkaido. Univ., 3AIST)GXXXG モチーフによる膜貫通ヘリックスの二量体形成：会合トポロジー制御下での一分子 FRET 研究
GXXXG-mediated dimerization of transmembrane helices: single-molecule FRET detection with controlled association topology
Yoshiaki Yano, Yuta Watanabe, Katsumi Matsuzaki (Grad. Sch. Pharm Sci., Kyoto Univ.)– S125 –3Pos091

3Pos092

脂質膜分子による上皮成長因子受容体の膜近傍ドメイン構造制御機構
Conformational regulation of the juxtamembrane domain of epidermal growth factor receptor by membrane lipid molecules
Ryo Maeda1, Takeshi Sato2, Yasushi Sako1 (1Cellular Informatics Lab., RIKEN, 2Inst. for Protein Research, Osaka Univ.)
ESR による銅ポンプ P 型 ATPase における 金属イオン配位子の動的構造：ATP 効果の研究
Structural dynamics in metal ion coordination of copper pump P-type ATPase as studied by EPR spectroscopy: Effect of ATP
Satoshi Yasuda1,2, Naoyuki Kuwabara3, Shoji Ueki4, Toshiaki Arata1,5 (1Dept. Biol. Sci., Grad. Sch. Sci. Osaka Univ., 2Asahikawa Med. Univ.,
3PF, KEK, 4Tokushima-Bunri Univ., 5Ctr. Adv. High Mag. Field Sci., Grad. Sci. Osaka Univ.)

3Pos093

高速 AFM による ABC タンパク質の動態観察
High-speed atomic force microscopy shows conformational changes of nucleic binding domains of ABC protein
Shohei Takigaura1, Mikihiro Shibata1,2, Kazuaki Yamahara3, Yasuhisa Kimura3, Kazumitsu Ueda3,4, Takayuki Uchihashi1,2, Toshio Ando2 (1Dep.

3Pos094

Phys. Kanazawa Univ., 2Bio-AFM FRC, 3Div. App. Lif. Sci. Kyoto Univ., 4iCeMs)
高速原子間力顕微鏡による電位依存性プロトンチャネルの直接観察
Direct observation of voltage-gated proton channels by high speed AFM
Hayato Yamashita1,2, Akira Kawanabe3,4, Yasushi Okamura3,4, Masayuki Abe1 (1Grad. Sch. of Eng. Sci., Osaka Univ., 2PRESTO, JST, 3Grad. Sch.

3Pos095

of med., Osaka Univ., 4CREST, JST)
多剤輸送担体 EmrE の基質結合エントロピー利得に対する水分子の寄与
Contribution of water molecules for the gain in the substrate binding entropy to multidrug resistance transporter, EmrE
Kazumi Shimono1, Keisuke Matsuda1, Shoko Suzuki1, Kaho Yajima1, Sakiyo Yamamoto1, Tomomi Kimura-Someya2,3, Mikako Shirouzu2,3,

3Pos096

Shigeyuki Yokoyama2,4, Seiji Miyauchi1 (1Fac. Pharm. Sci., Toho Univ., 2RIKEN SSBC, 3RIKEN CLST, 4RIKEN Struct. Biol. Lab.)
cd1NiR:cNOR 複合体構造を安定化する相互作用の理論解析
Theoretical analysis of interaction that stabilizes cd1NiR:cNOR complex structure
Kenta Yamada1, Takaharu Mori1, Kiyoshi Yagi1, Takehiko Tosha2, Yoshitsugu Shiro2, Yuji Sugita1,3,4,5 (1RIKEN TMS, 2RIKEN BSL, 3RIKEN

3Pos097

AICS, 4RIKEN QBiC, 5RIKEN iTHES)
3 次元立体構造が不明の膜タンパク質に対する耐熱化置換体の特定
Identification of Thermostabilizing Mutations for a Membrane Protein Whose Three-Dimensional Structure is Unknown
Yuta Kajiwara1, Satoshi Yasuda2,3, Yuuki Takamuku2, Takeshi Murata2,3,5, Masahiro Kinoshita4 (1Graduate School of Energy Science, Kyoto
University, 2Graduate School of Science, Chiba University, 3Molecular Chirality Research Center, Chiba University, 4Institute of Advanced Energy,

3Pos098

Kyoto University, 5JST, PRESTO)
In silico screening of novel stress response factors regulated by mitochondrial inner membrane proteases
Kenichiro Imai, Yoshinori Fukasawa, Kentaro Tomii, Paul Horton (AIRC, AIST)

核酸：相互作用・複合体形成 / Nucleic acid: Interaction & Complex formation
3Pos099

MD シミュレーションを用いた RNA 二重らせん構造の熱安定性予測
Predicting RNA Duplex Dimerization Free-Energy Changes upon Mutations Using Molecular Dynamics Simulations
Shun Sakuraba1, Kiyoshi Asai1,2, Tomoshi Kameda2 (1Grad. Sch. Frontier Sci., Tokyo Univ., 2AI center, AIST)

3Pos100

3Pos101

3Pos102

3Pos103

単層および多層カーボンナノチューブ上における蛍光 DNA の蛍光強度の塩基配列依存性
Base sequence dependence for fluorescence intensity of fluorescent dye-labeled DNA on single- and multi-walled carbon nanotubes
Shusuke Oura, Kazuo Umemura (Tokyo Univ. of Sci.)
メタダイナミクスとアルケミカル変換法を用いた定量的結合活性予測
Approach to the quantitative prediction of the binding affinity using metadynamics and alchemical transformation
Yoshiaki Tanida, Azuma Matsuura (FUJITSU LABORATORIES LTD.)
紫外線損傷 DNA における Flippling 機構
On the Flipping-out mechanism of the UV-induced DNA damage
Ryuma Sato, Ryuhei Harada, Yasuteru Shigeta (Center of comp. Sci., Univ. Tsukuba)
Comparison of Multi-Dyes Quenching by Single-Walled Carbon Nanotube Dispersion with Single Stranded DNA
Ying Tan, Katsuki Izumi, Kazuo Umemura (Tokyo University of Science)

電子状態 / Electronic state
3Pos104

3Pos105

3Pos106

サイトキニン脱水素酵素における独特なフラビン-基質配置に関する量子化学的研究
Quantum chemical study on unusual flavin-substrate alignment in cytokinin dehydrogenase
Kyosuke Sato (Dept. Mol. Physiol., Facult. Life Sci., Kumamoto Univ.)
生体分子の電子状態解析のための大規模第一原理 DFT 計算手法の開発
Large-scale DFT calculation method for electronic-structure analysis of biomolecules
Ayako Nakata1, Takao Ostuka2, David R. Bowler3, Tsuyoshi Miyazaki1 (1NIMS, 2RIKEN, 3UCL)
生体分子系における定温オーダーＮ法第一原理分子動力学計算
Constant temperature order-N first-principles molecular dynamics calculations of biomolecular system and short-time behavior
Takao Otsuka1, Makoto Taiji1, David R. Bowler2, Tsuyoshi Miyazaki3 (1RIKEN QBiC, 2UCL, 3NIMS)– S126 –水･水和･電解質 / Water & Hydration & Electrolyte
3Pos107

3Pos108

3Pos109

3Pos1103Pos111

両親媒性抽出剤を用いた水相から有機相へのリン酸化合物の抽出
Extraction of phosphoric compounds from aqueous phase into an organic phase with an amphiphilic extractant
Hideyuki Komatsu (Bioinfo. & Biosci., Kyushu Inst. Tech.)
リン酸イオンの水和エネルギー空間分割解析
Spatial-Decomposition Analysis of Hydration Energy of Phosphate Ions
George Mogami1, Nobuyuki Matubayasi2, Makoto Suzuki1 (1Grad. Sch. Eng., Tohoku Univ., 2Grad. Sch. Eng. Sci., Osaka Univ.)
実効相互作用を用いた電解質中のマクロアニオンの分子シミュレーション
Molecular simulation of macroanions in an electrolyte solution based on the effective potential
Ayumi Suematsu, Ryo Akiyama (Dept. Chem., Kyushu Univ.)
MM/3D-RISM 法を用いたシクロデキストリン誘導体とロクロニウム臭化物の結合自由エネルギーの予測
Estimation of binding free energies for inclusion processes of Rocuronium bromide by cyclodextrin derivatives using MM/3D-RISM method
Yuji Hayashino1, Masatake Sugita1, Fumio Hirata2, Takeshi Kikuchi1 (1Dept. of Bioinfo., Col. Life Sci., Ritsumeikan Univ., 2Toyota Phys. & Chem.
Res. Inst.)
ボルンエネルギーとクーロンエネルギー間のバランスの物理的理解と計算指針
Physical understanding and computational guideline for the balance between Born and Coulomb energies
Dan Parkin, Yukinobu Mizuhara, Mitsunori Takano (Dept. of Pure & Appl. Phys., Waseda Univ.)

分子遺伝学･遺伝子発現 / Molecular genetics & Gene expression
3Pos1123Pos113

3Pos114

3Pos115

何故無細胞タンパク質合成系においてはそんなに多くのリボソームが必要なのか？
Why we need so many ribosomes in cell-free protein synthesis?
Yue Xu, Yi Zhang, Hiroyuki Noji (Grad. Sch. Eng., Univ. Tokyo)
一細胞トランスクリプトーム解析へ向けた、PCR を含まないライブラリー調製
Amplification-free library construction for single-cell transcriptome analysis
Tetsuo Fujinami, Yusuke Oguchi, Mai Yamagishi, Yoshitaka Shirasaki, Sotaro Uemura (Grad. Sch. Sci. Univ. Tokyo)
無細胞タンパク質合成系に向けた最良 T7 プロモーター配列の探索
Improvement of T7 promoter sequence for cell-free protein synthesis
Tomoya Nishimura1, Yi Zhang2, Hiroyuki Noji2 (1Undergrad. Sch. Eng., Univ. Tokyo, 2Sch. Eng., Univ. Tokyo)
細胞集積度によるパラクラインシグナリングの制御により遺伝子発現の安定性が変化した
Paracrine signaling modulated by the accumulation of cells altered the stability of gene expression
Mai Yamagishi1,3, Yoshitaka Shirasaki1,3, Yutaka Hori2, Nobutake Suzuki1, Osamu Ohara3, Sotaro Uemura1 (1Grad. Sci. Sci., The Univ. of Tokyo,
2Fac. Sci. Technol., Keio Univ., 3IMS, RIKEN)

3Pos116
3Pos117

Causal role of DNA methylation ?: A computational model
Ashwin S.S, Masaki Sasai (Dept. of Computational Sciences and Engineering & Dept. of Applied Physics, Nagoya Univ., Nagoya)
An in silico Approach to Investigating Gene Variants of Unknown Significance in a Clinical Context
Stefanie S. Portelli1, Elizabeth N. Robertson1,2, Yaxin Lu1, Murat Kekic1, Brett D. Hambly1, Richmond Jeremy1,2,3 (1Univ. Sydney, 2Royal Prince

3Pos118

3Pos119

Alfred Hospital, 3Baird Institute)
遺伝子発現におけるポリアミンの DNA 高次構造との関係性
Relationship between DNA higher order structure and Gene-Expression with Polyamines
Ai Kanemura1, Yuko Yoshikawa1, Takahiro Kenmotsu1, Wakao Hukuda2, Kenichi Yoshikawa1 (1Grad. Sch. Life Med. Sci., Univ. Doshisha, 2Coll.
Life Sci., Univ. Ritsumeikan)
Guillardia theta におけるロドプシン様遺伝子群の発現解析
Expression analysis of microbial rhodopsin-like genes in Guillardia theta
Masae Konno1,3, Keiichi Inoue1,2, Hideki Kandori1,3 (1Grad. Sch. Eng., Nagoya Inst. Tech., 2PRESTO, JST, 3OPTRC, Nagoya Inst. Tech.)

筋肉 / Muscle
3Pos121

細胞性粘菌の Tyr143 変異アクチンのカルボキシ末端領域にある Phe352、Met355 と Trp356 の側鎖の二形性
Dimorphism of the side-chains of Phe352, Met355, and Trp356 in the carboxyl-terminal region of Dictyostelium actin mutants

3Pos122

Yuki Gomibuchi1, Taro Q.P. Uyeda2, Takeyuki Wakabayashi1 (1Teikyo Univ., 2Waseda Univ.)
筋収縮制御メカニズムの解明を目指した細いフィラメントの立体構造解析
CryoEM structural analysis of muscle thin filament composed of actin filament, tropomyosin and troponin

3Pos123

Yurika Yamada1, Keichi Namba1,2, Takashi Fujii1 (1Grad. Sch. of Frontier Biosci., Osaka Univ., 2RIKEN QBiC)
中性子準弾性散乱により明らかとなった心筋症原因変異がもたらすトロポニンの動力学異常
Effects of a cardiomyopathy-causing mutation on the internal dynamics of troponin revealed by quasielastic neutron scattering
Tatsuhito Matsuo1, Taiki Tominaga2, Kaoru Shibata3, Satoru Fujiwara1 (1QST/J-PARC, 2CROSS-Tokai, 3J-PARC)– S127 –3Pos124

ウニのコネクチン様タンパク質の構造解析
Sequential analysis of connectin-like protein in sea urchin
Sumiko Kimura1, Akira Hanashima2, Maki Yamaguchi1, Toshiko Yamazawa1, Tetsuo Ohno1, Naoya Nakahara1, Mika Taguchi1, Shigeru

3Pos125

3Pos126

Takemori1 (1Dept. Mol. Physiol., Jikei Univ. Sch. Med., 2First Dept. Physiol., Kawasaki Med. Sch.)
Mg ポリマー再考
Revisiting “Mg-Polymer”
Mahito Kikumoto, Shuuichi Takeda, Yuichiro Maeda (Structural Biology Research Center, Nagoya-Univ.)
細いフィラメント上のトロポミオシンのモデル：スピンラベル ESR 距離マップ
Modeling for tropomyosin position in the thin filament by distance measurements using spin-labeling dipolar EPR spectroscopy
Keisuke Ueda1,2, Yoshiki Tsujimoto2, Hiroaki Yamashita2, Kouichi Sakai2, Shoji Ueki4, Masao Miki3, Toshiaki Arata2,5 (1CLIST, Riken-Yokohama,
2Dept. Biol. Sci. Grad. Sch. Osaka Univ., 3Univ. Fukui, 4Tokushima-Bunri Univ., 5Ctr. Adv. High Mag. Field Sci., Grad. Sci. Osaka Univ.)

3Pos127

3Pos128

3Pos129

3Pos130

筋原線維懸濁液の ATP 分解素過程中のプロトン NMR 緩和経過
Spin-spin relaxation of 1H NMR signals from myofibril suspension during cross-bridge cycling
Tetsuo Ohno, Hitomi Sano (Dept. Physiol., The Jikei Univ. Sch. Med.)
骨格筋タンパク質と水の相互作用を融点から探る
Interaction between water and myoproteins revealed by melting points
Naoya Nakahara1, Tetsuo Ohno1, Masako Kimura2, Sumiko Kimura1, Shigeru Takemori1 (1Jikei Univ. Sch. Med., 2Kagawa Nutri. Univ.)
T-plastin の 2 つのアクチン結合ドメインとアクチンフィラメントとの結合性の比較
Comparison of binding affinities of two actin-binding domains of T-plastin to actin filament
Taiki Hirate, Atsusi Ooi, Tsuyoshi Okagaki (Dept., Bioresources, Mie Univ.)
心筋細胞集団の伝搬のゆらぎの局所・全体相関の解明のためのオンチップ心筋細胞ネットワーク解析技術の開発
Development of On-chip Cardiomyocyte Network Analysis Assay for Understanding of Fluctuation Correlation in Cell-to-cell Conduction
Naoki Takahashi1, Hideyuki Terazono2, Masao Odaka2, Kenji Matsuura2, Akihiro Hattori2, Kenji Yasuda1 (1Dept. Physics, Waseda Univ.,
2WASEDA Biosci. Res. Inst. Singapore(WABIOS), Waseda Univ.)

3Pos1313Pos132

肥大型心筋症特異的なトロポミオシン変異体(V95A,D175N)のアクトミオシン収縮速度・収縮力への異なる影響
Tropomyosin's HCM mutants (V95A, D175N) differently affect the actomyosin sliding velocity and force
Shuya Ishii1, Shin’ichi Ishiwata2, Masataka Kawai3 (1Sch. Adv. Sci. Eng., Waseda Univ., 2Fac. Sci. Engn., Waseda Univ., 3Coll. Med., Univ. Iowa)
温めた心筋細胞に備わった収縮振動は遅い Ca2+変動に対して周期を一定に保つ
Contractive oscillations intrinsic to heating cardiomyocytes maintain the period against late Ca2+ variations

3Pos133

3Pos134

Seine A. Shintani1,2, Takumi Washio3, Hideo Higuchi1 (1Dept. Physics, Univ. Tokyo, 2JSPS Research Fellow, 3Dept. Human and Engineered
Enviromental, Univ. Tokyo)
周期性伸展刺激における伸展周期と心筋細胞の応答の関係
Relation between stretch cycles and response of cardiomyocytes in cyclic stretch stimulation
Chiho Nihei, Tomoyuki Kaneko (LaRC, Grad. Sci. Eng., Hosei Univ.)
Mg2+/Ca2+ 交換及び温度変化による F-アクチンの三次構造変化
Tertiary structure of F-actin affected by Mg2+/Ca2+ and temperature
Takaya Yamaguchi, George Mogami, Makoto Suzuki (Grad. Sch. Eng., Univ. Tohoku)

分子モーター / Molecular motor
3Pos135

Does homo hexamer function as a stator of rotary motor?

3Pos136

Aiko Endo1, Junichi Kishikawa2, Ken Yokoyama2 (1Grad. Sch. Biochem., Kyoto sangyo Univ., 2Dept. Mol. Biosci., Kyoto sangyo Univ.)
全原子 MD と粗視化 MD を組み合わせたマルチスケール MD 解析による V1-ATPase の回転機構の解明
Rotation mechanism of V1-ATPase elucidated by multi-scale MD analysis
Yuta Isaka1, Toru Ekimoto1, Yuichi Kokabu1, Takeshi Murata2,3 (1Grad. Sch. of Med. Life Sci., Yokohama City Univ., 2Fac. of Sci., Chiba Univ.,
3JST, PRESTO)

3Pos137

Probing the biophysical properties of a Thermoalkaliphilic F1 ATPase gives insight into adaptation and regulation
Duncan G. G. McMillan1, Rikiya Watanabe1, Hiroshi Ueno1, Gregory M. Cook2, Hiroyuki Noji1 (1Dept. of Applied Chemistry, The Univ. of Tokyo,
2Dept. of Microbiology and Immunology, Univ. of Otago, Dunedin, New Zealand)

3Pos138

高速 AFM で明らかにする回転軸の無い腸内連鎖球菌由来 V1-ATPase の一方向的協同性度合
The Extent of Unidirectional Cooperativity in Rotorless Enterococcus hirae V1-ATPase Revealed by High-speed AFM
Motonori Imamura1, Kazuya Nakamoto2, Shintaro Maruyama2, Fumihiro Kawai3, Ryota Iino3, Takayuki Uchihashi1,4,5, Takeshi Murata2,6, Toshio
Ando1,4,5 (1Bio-AFM FRC, 2Grad. Sch. Sci., Chiba Univ., 3Okazaki Inst. Integ. BioSci., IMS, NINS, 4Dept. Phys., Kanazawa Univ., 5CREST, JST,
6PRESTO, JST)

3Pos139リン酸結合蛋白を封入したフェムトリットル体積のドロップレットアレイによる無機リン酸検出
Detection of inorganic phosphate by phosphate binding protein encapsulated in femtoliter droplet arrays
Masayuki Higuchi1, Kazuhito V. Tabata2,4, Hiroyuki Noji2, Tomoko Masaike1,3,4 (1Dept. Appl. Biol. Sci., Tokyo Univ. of Sci., 2Dept. Appl. Chem.,
Sch. of Eng., Univ. of Tokyo, 3Res. Inst. for Sci. and Tech., Tokyo Univ. of Sci., 4PRESTO, JST)– S128 –3Pos140

Unveiling the chemomechanical coupling of F1 ATPase of Paracoccus denitrificans
Mariel Zarco - Zavala1, Duncan G G McMillan1, Toshiharu Suzuki1, Hiroshi Ueno1, Francisco Mendoza-Hoffmann2, Jose J. Garcia-Trejo2,

3Pos141

Hiroyuki Noji1 (1Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, 2Departament of Biology, Chemistry
Faculty, National Autonomous University of Mexico)
腸球菌由来 ADP 結合型 V1-ATPase の X 線結晶構造解析
Crystal structures of the ADP-bound V1-ATPase from Enterococcus hirae
Kano Suzuki1, Kenji Mizutani1,2,3, Shintaro Maruyama1, Kazumi Shimono4, Fabiana L. Yakushiji1, Eiro Muneyuki5, Yoshimi Kakinuma6, Yoshiko
Ishizuka-Katsura7, Mikako Shirouzu7, Shigeyuki Yokoyama8, Ichiro Yamato3, Takeshi Murata1,2,7,9 (1Chiba Univ., 2Mol. Chirality Res. Center,
Chiba Univ., 3Dept. Biol. Sei. Tech., Tokyo Univ. of Science, 4Faculty of Pharm. Sci., Toho Univ., 5Faculty of Sci. and Eng., Chuo Univ., 6Faculty of

3Pos142Agri., Ehime Univ., 7DSSB, RIKEN, 8Struct. Biol. Lab., RIKEN, 9JST, PRESTO)Enterococcus hirae 由来 V1-ATPase アルギニンフィンガー変異体が示す特異な回転特性
Arginine finger mutant of Enterococcus hirae V1-ATPase shows unusual rotational behaviors
Tatsuya Iida1, Yoshihiro Minagawa2, Hiroshi Ueno2, Takeshi Murata3, Ryota Iino1,4,5 (1SOKENDAI (The Grad. Univ. for Adv. Stud.), 2The Univ.
Tokyo, 3Chiba Univ., 4Inst. for Mol. Sci., 5Okazaki Inst. for Integr. Biosci.)

3Pos143

Structural analysis by NMR on C-terminal region of FliG, an essential motor component of Vibrio Na+-driven flagella
Yohei Miyanoiri1, Yuuki Nishino2, Mizuki Gohara2, Atsushi Hijikata3, Yasuhiro Onoue2, Seiji Kojima2, Tsuyoshi Shirai3, Masatsune Kainosho1,

3Pos144

Michio Homma2 (1SBRC. Grad. Sch. Sci., Nagoya Univ., 2Biosci. Grad. Sch. Sci., Nagoya Univ., 3Biosci. Nagahama Inst. Biosci. Tech.)
サルモネラ属菌 FliFG 融合変異型べん毛モーターの動態機能計測
Functional analysis of a FliFG deletion-fusion mutant flagellar motor
Tomofumi Sakai1, Koichiro Mori1, Yumi Inoue1, Tomoko Miyata1, Naoya Terahara1, Yusuke Morimoto2, Takayuki Kato1, Keiichi Namba1,2,

3Pos145

3Pos146

3Pos147
3Pos148
3Pos149

Tohru Minamino1 (1Grad. Sch. Frontier BioSci., Osaka Univ., 2RIKEN QBiC)
光ピンセットを用いたバクテリアべん毛モーターの最大トルクの計測
Maximum torque generated by the bacterial flagellar motor measured by optical tweezers
Taishi Kasai1, Yoshiyuki Sowa1,2 (1Reserch center for Micro-Nano Tech. Hosei Univ., 2Dept. Frontier Biosci., Hosei Univ.)
べん毛モーターのトルク特性とその個体差の精密測定
Precise measurement of torque characteristics and individual variability of bacterial flagellar motor
Kento Sato, Shuichi Nakamura, Seishi Kudo, Shoichi Toyabe (Grad. Sch. Eng., Tohoku Univ.)
The mechanism of Vibrio alginolyticus polar flagellum growth
Chien-Jung Lo, Meiting Chen (Department of Physics, National Central University)
Functional Analysis of Slow-Motile Mutations in Flagellar Stator MotA/B of Salmonella
Seyedehnoorolhoda Shajaripourjaberi, Naoya Terhara, Tohru Minamino (frontier biosciences)
Rng2 による F-アクチンとミオシン間の協同的相互作用の制御
Regulation of cooperative interaction between myosin and F-actin by Rng2
Taiga Imai1, Masak Takaine2, Kentaro Nakano2, Osamu Numata2, Taro Uyeda3, Kiyotaka Tokuraku1 (1Muroran institute of technology, 2University

3Pos150of Tsukuba, 3Waseda University)
SH1 ヘリックス内に変異をもつミオシン II はアクチンフィラメントの滑りの活性化エネルギーを減少させる
Myosin II SH1 helix mutant lowers the activation energy for sliding of Factin

3Pos151

Shigeru Chaen1, Kotomi Shibata1, Tsubasa Koyama1, Atsushi Suenaga1, Sosuke Iwai2 (1Dept. Biosci. Nihon Univ., 2Dept. Biol. Hirosaki Univ.)プログラム可能なミオシンアセンブリの設計と高解像 1 分子イメージング
Design of a programmable myosin motor assembly and nanometer-precision single-molecule imaging

3Pos152

3Pos153

Masashi Ohmachi1, Keisuke Fujita1, Keigo Ikezaki2, Toshio Yanagida1,3, Mitsuhiro Iwaki1,3 (1QBiC, RIKEN, 2Univ. of Tokyo, 3Osaka Univ.)
単純化された筋繊維の計算モデルを用いた筋収縮におけるバイアスブラウン運動の寄与の研究
Contribution of biased Brownian motion in muscle contraction studied by a simplified computational model of muscle fiber
Daisuke Watanabe, Masaki Sasai, Tomoki P. Terada (Dept. Comput. Sci. Eng., Grad. Sch. Eng., Nagoya Univ.)
Rng2 によるアクチンフィラメントの構造変化と、HMM で駆動されるアクチン運動の協同的阻害
Structural changes of actin filaments induced by Rng2, and the resultant inhibition of actin movement on HMM
Yuki Hayakawa1, Keiko Hirose2, Masafumi Yamada2, Kien X. Ngo1, Noriyuki Kodera4, Masak Takaine3, Kentaro Nakano3, Osamu Numata3, Taro

3Pos154

Uyeda1,2 (1dep physics, Waseda Univ., 2Biomed Res Inst, AIST, 3Grad. School of Life and Environ Sci., Univ. Tsukuba, 4Bio-AFM Res Ctr,
Kanazawa Univ.)
Effect of external mechanical stress on collective motion of microtubules
Tamanna Ishrat Farhana1, Arif Md. Rashedul Kabir2, Daisuke Inoue2, Kazuki Sada1,2, Akira Kakugo1,2 (1Grad. Sch. Chem. Sci. & Eng., Hokkaido

3Pos155

Univ., 2Fac. of Sci., Hokkaido Univ.)
微小管内 GDP-チューブリンの精密な周期決定
Determination of accurate axial tubulin repeat in GDP-microtubules
Shinji Kamimura1, Hiroshi Imai1, Toshiki Yagi2, Tomohiro Shima3,4, Yasushi Okada4,5, Hiroyuki Iwamoto6 (1Dept. Biol. Sci., Chuo University,
2Dept. Life Sci., Pref. Univ. Hiroshima, 3Dept. Biol. Sci., Univ. Tokyo, 4Lab. Cell Polarity Regulation., Qbic, RIKEN, 5Dept. Phys., Univ. Tokyo,
6Life & Environmental Div., SPring-8)– S129 –3Pos156

高精度な微小管分離に向けた微小管の持続長設計
Design of Microtubule Persistence Length Toward High-precision Microtubule Sorting
Naoto Isozaki1, Hirofumi Shintaku1, Hidetoshi Kotera1, Taviare L. Hawkins2, Jennifer L. Ross3, Ryuji Yokokawa1 (1Kyoto University, 2University

3Pos157

of Wisconsin - La Crosse, 3University of Massachusetts - Amherst)
Regulated swarming of molecular robots prepared from a DNA programmed biomolecular motor system
Jakia Jannat Keya1, Ryuhei Suzuki1, Arif Md. Rashedul Kabir2, Daisuke Inoue2, Kazuki Sada1,2, Akinori Kuzuya3, Akira Kakugo1,2 (1Graduate

3Pos158

3Pos159

School of Chemical Sciences and Engineering, Hokkaido University, 2Faculty of Science, Hokkaido University, 3Faculty of Chemistry andBioengineering, Kansai University)
構成論的手法を用いた鞭毛の機能的再構築
Functional reconstitution of flagellar axonemes by self-organization of microtubules, dynein docking complex and outer arm dyneins
Misaki Shiraga1,2, Jyunya Kirima1,2, Hiroaki Kojima1, Kazuhiro Ooiwa1,2 (1Adv. ICT Res. Inst. NICT, 2Grad. Sch. Sci., Univ. Hyogo)
電子顕微鏡トモグラフィーによって明らかになった細胞質ダイニンのモータードメインの配置
Orientation of two motor domains of cytoplasmic dynein characterized using electron computed tomography
Kotaro Koyasako1, Shiori Toba2, Shinji Hirotsune2, Takuo Yasunaga1 (1Department of Bioscience and Bioinformatics, Faculty of Computer

3Pos160

Science and Systems Engineering, Kyushu Institute of Technology, 2Department of Genetic Disease Research, Osaka City University Graduate
School of Medicine)
ポリグルタミン酸化酵素欠損マウスの気管繊毛が示す異常な跳ね上がり運動
Abnormal “hopping” of mouse tracheal cilia deficient in tubulin polyglutamylation
Masayuki Shiina1, Toshihito Iwase1, Masaaki Suegara1, Haruka Kanno1, Takanobu A Katoh2, Mitsutoshi Setou3, Takayuki Nishizaka2, Koji

3Pos161

3Pos162

Ikegami3, Tomoko Masaike1,4 (1Dept. Appl. Biol. Sci., Tokyo Univ. of Sci., 2Dept. Phys., Gakushuin Univ., 3Hamamatsu Univ. Sch. Med., 4PRESTO,
JST)
クラミドモナス鞭毛の波形変化に関わるタンパク質の探索
Searching a putative protein responsible for switching waveform of Chlamydomonas flagella
Junya Kirima1, Misaki Shiraga1, Hiroaki Kojima2, Kazuhiro Oiwa1,2 (1Grad. Sch. Life Sci., Univ. Hyogo, 2Adv. ICT Res. Inst., NICT)
バクテリア・カビ由来セロビオヒドロラーゼの結合、解離、プロセッシブ運動の一分子蛍光観察
Single-molecule fluorescence analysis of binding, dissociation, and processive movement of bacterial and fungal cellobiohydrolases
Daiki Ishiwata1, Akihiko Nakamura1,2, Tomoyuki Tasaki3, Akasit Visootsat4, Maximilien Morice5, Ryota Iino1,2,6 (1Sch. phys. sci., SOKENDAI(The Graduate University for Advanced Studies), 2Okazaki Inst. for Integr. Biosci., 3Sch. of Engi., Univ. Tokyo, 4Fac. Sci., Univ. Kasetsart, 5Chimie

3Pos163

ParisTech., 6Inst. for Mol. Sci.,)
分子モーター RecBCD による混雑環境下でのタンパク質-DNA 複合体の除去
Sequential eviction of crowded nucleoprotein complexes by the RecBCD molecular motor

3Pos164

Tsuyoshi Terakawa1, Redding Sy1,2, Silverstein Timothy1, Greene Eric1 (1Columbia Univ., 2Univ. of California)
RHAU ヘリカーゼがグアニン４重鎖構造の安定性を調節するメカニズムの一分子研究
RHAU helicases regulate G4 stability during the ATPase cycle revealed from single-molecule analysis

3Pos165

Huijuan You1, Jie Yan1,2 (1MBI, National University of Singapore, 2Dep. of Physics, National University of Singapore)
転写バーストは DNA 上の RNA ポリメラーゼの相互作用によって内因的に引き起こされるTranscriptional bursting is intrinsically caused by interplay between RNA polymerases on DNA

3Pos166

Keisuke Fujita1,2, Mitsuhiro Iwaki1,2, Toshio Yanagida1,2 (1QBiC, RIKEN, 2Grad. Sch. of Front. Biosci., Osaka Univ.)
金ナノプローブで明らかにされたリニア分子モーター霊菌 Serratia marcescens 由来キチナーゼ A の 1 nm ステップ運動
One nanometer steps in the motion of a linear molecular motor Serratia marcescens chitinase A resolved by gold nanoprobe
Akihiko Nakamura1,2, Ryota Iino1,2,3 (1Okazaki inst. for Integrative Bioscience, 2The Graduate University for Advanced Studies (SOKENDAI),
3Institute for Molecular Science)

細胞生物学 / Cell biology
3Pos167

海洋性ビブリオ菌のべん毛形成に関わる DnaJ ファミリータンパク質 SflA のペリプラズム領域の構造特性
Structural property of the periplasmic TPR domain of SflA, a DnaJ family protein involved in flagellation of Vibrio alginolyticus
Mayuko Sakuma1,2, Satoshi Inaba2, Shoji Nishikawa3, Takehiko Nishigaki2, Seiji Kojima2, Katsumi Imada3, Michio Homma1,2 (1Radioisotpe Res.

3Pos168

3Pos169

3Pos170

Cent., Nagoya Univ., 2Div. Biol. Sci. Grad. Sch. Sci., Nagoya Univ., 3Dept. of Macromol. Sci., Grad. Sch. Sci., Osaka Univ.)
FlhF がもつ GTPase モチーフへの変異によるビブリオ菌極べん毛数と位置への影響
Effect of mutations in the GTPase motif of FlhF on the number and location of the polar flagellum of Vibrio alginolyticus
Shota Kondo, Michio Homma, Seiji Kojima (Division of Biological Science, Graduate School of Science, Nagoya University)
細菌 Rhomboid プロテアーゼ GlpG の生理的基質の探索：GlpG のべん毛 III 型分泌装置機能への関与の可能性
Screening of physiological substrates of E. coli rhomboid protease GlpG: possible involvement of GlpG in the flagellar function
Yohei Hizukuri, Kosuke Terushima, Yoshinori Akiyama (Inst. Virus Res., Kyoto Univ.)集合に共役したべん毛モーター固定子 MotB のペリプラズム領域における構造変化の変異体解析
Mutational studies of the assembly-coupled conformational change in the periplasmic region of a flagellar stator protein MotB
Seiji Kojima1, Masato Takao2, Gaby Almira3, Ikumi Kawahara3, Mayuko Sakuma1, Michio Homma1, Chojiro Kojima3,4, Katsumi Imada2 (1Div. ofBiol. Sci., Grad. Sch. of Sci., Nagoya Univ., 2Dept. of Macromol. Sci., Grad. Sch. of Sci., Osaka Univ., 3Inst. for Prot. Res., Osaka Univ., 4Grad. Sch.
of Eng., Yokohama National Univ.)– S130 –3Pos171

細菌べん毛モーター固定子蛋白質 MotA および回転子蛋白質 FliF の構造解析のための条件検討
Screening for the structural analysis of the stator and the rotor proteins MotA and FliF in the bacterial flagellar motor
Norihiro Takekawa1, Mayuko Sakuma2,3, Erika Yamaguchi2, Seiji Kojima2, Michio Homma2, Katsumi Imada1 (1Dept. of Macromol. Sci., Grad.

3Pos172

Sch. of Sci., Osaka Univ., 2Div. of Biol. Sci., Grad. Sch. of Sci., Nagoya Univ., 3Radioisotope Res. Cent., Nagoya Univ.)
FlhA の構造変換がべん毛蛋白質輸送順序の決定に重要である
Conformational rearrangements of FlhA is critical for ordered protein export during flagellar assembly
Yumi Inoue1, Yuya Ogawa2, Miki Kinoshita1, Katsumi Imada2, Keiichi Namba1,3, Tohru Minamino1 (1Grad. Sch. Frontier Biosci., Osaka Univ.,
2Grad. Sch. Sci., Osaka Univ., 3QBiC, RIKEN)

3Pos173

サルモネラべん毛モーターにおける MotA Met-206 の役割
Roles of MotA Met-206 in rotation and assembly of the Salmonella flagellar motor
Yuya Suzuki1, Kodai Oono1, Shuichi Nakamura1, Fumio Hayashi2, Kenji Oosawa3, Yusuke V. Morimoto4, Seishi Kudo1 (1Grad. Sch. Eng., Tohoku

3Pos174

Univ., 2Center for Inst. Anal., 3Div. Mol. Sci., Fac. Sci. and Tech., Gunma Univ., 4QBiC, Riken)
細菌べん毛 III 型タンパク質輸送の in vitro 再構築
In vitro reconstitution of the bacterial flagellar type III protein export
Hiroyuki Terashima1, Akihiro Kawamoto2, Chinatsu Tatsumi1, Keiichi Namba2,3, Tohru Minamino2, Katsumi Imada1 (1Grad. Sch. Sci., Osaka

3Pos175

Univ., 2Grad. Sch. Front. Biosci., Osaka Univ., 3Quant. Bio. Cent., Riken.)
反転膜を用いたべん毛 III 型蛋白質輸送の蛍光による検出
Fluorescence detection of the flagellar type III protein export using the inverted membrane vesicles
Tsuyoshi Tono1, Hiroyuki Terashima1, Kazuhito Tabata2, Hiroshi Ueno2, Tomoki Matsuda3, Takeharu Nagai3, Hiroyuki Noji2, Katsumi Imada1

3Pos1763Pos177

3Pos178
3Pos179

3Pos180(1Grad. Sch. of Sci., Univ. Osaka., 2Sch. of Eng., Univ. of Tokyo, 3ISIR, Univ. Osaka.)
バクテリアべん毛輸送ゲート複合体の発現系の構築と精製
Expression and purification of the bacterial flagellar type III export gate complex
Takuma Fukumura1, Miki Kinoshita1, Keiichi Namba1,2, Tohru Minamino1 (1Grad. Sch. Frontier Biosci, Osaka Univ., 2QBiC, RIKEN)
三次元的形状解析を用いたらせん形細菌の構造的理解
3D microscopic observation of the cell shape of spiral shaped bacteria
Hajime Tahara, Shuichi Nakamura (Grad. Sch. Eng. Univ. Tohoku)
Coordinated cell-body rotation in spirochete motion
Kyosuke Takabe, Seishi Kudo, Shuichi Nakamura (Grad. Sch. Eng., Univ. Tohoku)
局所的な照明によって誘起されたスピロプラズマの遊泳方向の反転
Reversal motion of Spiroplasma induced by partial illumination
Tatsuro Itou1, Daisuke Nakane1, Wen Wang2, Takayuki Nishizaka1 (1Department of Physics, Gakushuin Univ., 2College of Life Sciences, Nanjing
Normal Univ.)
ロッド様に直線状で固く，野生型より長い変異型フックの金ナノ粒子標識によるべん毛モーターの高分解能回転計測
High resolution measurements of flagellar motor rotation by nanogold attached to a straight, solid hook mutant longer than the wild-type
Shuichi Nakamura1,2, Yusuke V. Morimoto2,3, Tohru Minamino2, Keiichi Namba2 (1Grad. Sch. Eng., Tohoku Univ., 2Grad. Sch. Frontier BioSci.,

3Pos1813Pos182

3Pos183

3Pos184

Osaka Univ., 3QBiC, Riken)
シュードモナス属べん毛モーターの回転計測
Rotation assay of the Pseudomonas flagellar motor
Taro Hariu, Takuto Tensaka, Seisi Kudo, Shuichi Nakamura (Grad. Sch. Eng., Tohoku Univ.)
ガラスに付着したビブリオ菌を用いたべん毛フリッキングの解析
Analysis of flagellar flicks of Vibrio cells stuck to a glass slide
Taichi Ohnuki, Shuichi Nakamura, Shoichi Toyabe, Seishi Kudo (Grad. Sch. Eng., Univ. Tohoku)
大腸菌の忌避刺激で見られた時間遅れは FliM の共同的な振る舞いにより説明される
Simulation of delays in repellent responses of Escherichia coli using a conformational spread model
Takashi Sagawa1, Yoshiyuki Sowa2, Ikuro Kawagishi2, Kazuhiro Oiwa1, Hiroaki Kojima1 (1Adv. ICT Res. Inst., NICT, 2Dept. of Front. Biosci.,
Hosei Univ.)
大腸菌二成分制御系 AtoS，AtoC の相互依存的細胞内局在Interdependent co-localization of the histidine kinase AtoS and the response regulator AtoC of Escherichia coli
Takahide Endo1, Yukiko Miyao1, Kentaro Yamamoto1, Masatoshi Nishikawa1, Yoshiyuki Sowa1,2, Ikuro Kawagishi1,2 (1Dept. Frontier Biosci.,

3Pos185

Hosei Univ., 2Research Center for Micro-Nano Technology, Hosei Univ.)
大腸菌は、アミノ酸種を識別する：データ駆動的アプローチにより明らかにする単細胞生物の化学知覚
Escherichia coli identify amino-acid species : unicellular organism’s chemical perception revealed by using a data-driven approach
Hiroto Tanaka1, Yasuaki Kazuta1, Tadashi Matsukawa1, Yasushi Naruse2, Yukihiro Tominari1, Masato Okada3, Yoshiyuki Sowa4, Ikuro

3Pos186

Kawagishi4, Kazuhiro Oiwa1, Hiroaki Kojima1 (1Adv ICT Res Inst, NICT, 2CiNet, NICT, 3Tokyo Univ., 4Housei Univ.)
コレラ菌走化性受容体 Mlp24 のアミノ酸受容能はカルシウムイオンで増強される
Ca2+ potentiates attractant responses to amino acids mediated by the chemoreceptor Mlp24 of Vibrio cholerae
So-ichiro Nishiyama1,2, Yohei Takahashi3, Katsumi Imada3, Ikuro Kawagishi1 (1Dept. Frontier Biosci., Hosei Univ., 2Res. Cen. Micro-Nano Tech.,Hosei Univ., 3Dep. Macromol. Sci., Grad. Sch. Sci., Osaka Univ.)– S131 –3Pos187

3Pos188

バクテリアのマグネットコンパスの人為的反転
Artificial polarity-reversal of bacterial magnetic compass
Seiji Iwata1, Shouhei Kuzoo1, Daisuke Nakane1, Azuma Taoka2,3 (1Dept. of Phys., Gakushuin Univ., 2Col. of Sci. Eng., Kanazawa Univ., 3Bio-AFM
Front. Res. Cent., Kanazawa Univ.)
Importance of receptor cooperativity on the switching coordination of flagellar motors on a single Escherichia coli cell

3Pos189

Yong-Suk Che1, Hiroto Takahashi2, Akihiko Ishijima1, Hajime Fukuoka1 (1Dept. Frontier Biosci., Osaka Univ., 2IMRAM, Tohoku Univ.)
変性蛋白質センサーとしての大腸菌ヒスチジンキナーゼ BaeS
The histidine kinase BaeS of Escherichia coli may sense denatured proteins

3Pos190

Tohru Umemura1, Yoshiyuki Sowa1,2, Ikuro Kawagishi1,2 (1Dept. Frontier Bio., Hosei Univ., 2Research Center for Micro-Nano Technology, Hosei
Univ.)
G タンパク質共役型受容体の動的なホモ・ヘテロダイマー形成：二色同時蛍光１分子観察による解析
Dynamic homo- and hetero-dimerizations of G-protein coupled receptors: An analysis by dual-channel single fluorescent molecule
observation

3Pos191

3Pos192

Rinshi Kasai1, Akihiro Kusumi1,2 (1Inst. Front. Med. Sci., Kyoto Univ., 2Membrane Cooperativity Unit, OIST)
共焦点画像解析と 1 分子計測を用いた ERK シグナル伝達系のボトルネックの解明
Unraveling origins of bottleneck effects for ERK signal transduction using confocal image analyses and single molecule imaging
Kazunari Mouri, Yasushi Okada (RIKEN QBiC)
三量体 G タンパク質の制御を介した走化性レンジの拡張機構
Dynamic range extension of eukaryotic chemotaxis via regulation of heterotrimeric G protein dynamics

3Pos193

Yukihiro Miyanaga1,2, Yoichiro Kamimura1,2, Masahiro Ueda1,2 (1Frontier Biosciences, Osaka Univ., 2QBiC, Riken)
走化性タンパク質の細胞内動態と細胞応答の同時計測
Simultaneous observation of the intracellular chemotactic proteins and the cellular behavior

3Pos194

Hajime Fukuoka1, Hiroto Takahashi2, Akihiko Ishijima1 (1Grad. Sch. Frontier Biosci., Osaka Univ., 2IMRAM, Tohoku Univ.)
ライブセルイメージングと薬剤実験に基づく動的な誘引物質勾配場における HL60 細胞の走化性運動の解析
Pharmacological and live-cell imaging analysis of chemotactic HL60 cells under dynamically changing chemoattractant gradient

3Pos195

3Pos196

Motohiko Ishida1, Akihiko Nakajima2, Satoshi Sawai1,2 (1Dept. Basic Sci., Grad. Sch. of Arts & Sci., Univ. of Tokyo, 2Research Center for Complex
Systems Biology, Grad. Sch. of Arts & Sci., Univ. of Tokyo)
マクロファージの走熱性メカニズムの解明
Thermotaxis mechanism of mouse macrophage
Hideo Saitou, Seine Shintani, Hideo Higuchi (Grad. Sch. Sci., The university of Tokyo)
免疫受容体シグナルを担う足場分子 LAT は細胞膜に繋留された小胞で機能する：1 分子イメジングによる解明
The immune signal adaptor molecule LAT works on cytoplasmic vesicles tethered to the plasma membrane: a single-molecule imaging
study
Koichiro M. Hirosawa1, Bo Tang2, Nao Hiramoto-Yamaki1,3, Kenta J. Yoshida1, Shohei Nozaki4, Takaaki Tsunoyama5, Kenichi G.N. Suzuki6,
Kazuhisa Nakayama4, Takahiro K. Fujiwara1, Akihiro Kusumi5 (1WPI-iCeMS, Kyoto Univ., 2College of Chemistry and Molecular Sciences, Wuhan

3Pos197

3Pos198
3Pos199

3Pos200

3Pos201
3Pos202

Univ., 3JSPS Research Fellow, 4Grad. Sch. Pharm., Kyoto Univ., 5OIST, 6NCBS-inStem, Bangalore, India.)
PIP3 と Ras の自己組織的な局在形成過程とその制御メカニズムの解析
Analysis of Self-organized Domain Formation and Regulation Mechanism of PIP3 and Ras
Seiya Fukushima1,2, Satomi Matsuoka2,3, Masahiro Ueda1,2,3 (1Grad. Sch. Sci., Univ. Osaka, 2QBiC, RIKEN, 3Grad. Sch. Frontier Biosciences.,
Univ. Osaka)
Controlling contractile instabilities in the actomyosin cortex
Masatoshi Nishikawa1,2,3,4, Sundar Naganathan2,3,4, Frank Julicher4, Stephan Grill2,3,4 (1Hosei University, 2TU-Dresden, 3MPI-CBG, 4MPI-PKS)
増殖中の培養細胞における遊離コレステロールの分布
Distribution of free cholesterol in MDCK cells during the migration on culture dishYoshikatsu Ogawa (AIST BMD)
獲得免疫応答における T リンパ球の単一細胞測定系の開発
Development of a single cell assay system for T lymphocytes in adaptive immunity
Hiroaki Machiyama1, Tomoyuki Yamaguchi1, Tomonobu Watanabe1,2, Hideaki Fujita1,2 (1IFReC, Osaka U., 2QBiC, RIKEN)Drug response of lymphocytic leukemia cells to anticancer drug is affected by experience of cell division prior to treatment
Akihisa Seita, Takahiro Yamada, Yuichi Wakamoto (Department of Basic Science, Graduate School of Arts and Science, University of Tokyo)
ErbB レセプターの相互リン酸化ネットワーク
Cell and signal specific phosphorylation networks of the ErbB receptor family
Hiraku Miyagi, Michio Hiroshima, Atsushi Mochizuki, Yasushi Sako (RIKEN)

生体膜・人工膜：構造・物性 / Biological & Artificial membrane: Structure & Property
3Pos203

膜活性ポリマーによる脂質二分子膜ナノディスクの形成
Lipid bilayer nanodiscs formed by designed membrane-active polymers
Kazuma Yasuhara, Jin Arakida, Masaya Inoue, Jun-ichi Kikuchi (Grad. Sch. Mat. Sci., Nara Inst. Sci. Tech.)– S132 –3Pos204

可溶性高分子で生成された水性相分離のマイクロ液滴内への生細胞の含有
Entrapment of Living Cells inside Micro-Droplet under Aqueous/Aqueous Segregation with Solvable Polymers
Tadashi Fujimoto1, Naoki Nakatani1, Kanta Tsumoto2, Chwen-Yang Shew3, Kenichi Yoshikawa1 (1Fac. Life Medical Sciences, Univ. Doshisha,
2Grad. Sch. Eng, Univ. Mie, 3Chemistry, City. Univ. New York)

3Pos205

3Pos206

粗視化モデルによる脂質分子の集合体の形成過程に関する理論的研究
Theoretical study on the process of the formation of lipid molecule cluster by coarse-grained model
Shogo Kinoshita, Satoshi Nakagawa, Makoto Wada, Seiichiro Ito, Kazutomo Kawaguchi, Hidemi Nagao (Nat. Sci. Kanazawa Univ.)
ベシクルに大小 2 種のコロイド粒子を内包させた系の相分離－朝倉・大沢理論の拡張―
Phase Separation of Two Kinds of Colloidal Particles in Giant Vesicles -Extension of Asakura-Osawa TheoryYuno Natsume1, Kazumi Itoh1, Yuhei Natsume2, Kensuke Kurihara3,4,5 (1Japan Women's Univ., 2Chiba Univ., 3Okazaki Institute for Integrative

3Pos207

3Pos208

3Pos209

Bioscience, 4Institute for Molecular Science, 5Research Center for Complex Systems Biology, The Univ. of Tokyo)
種々の脂質組成によるタンパク質内包リポソームの構造学的研究
Structural study of liposomes encapsulating proteins depending on lipid composition and species
Kosuke Takahashi, Mitsuhiro Hirai (Grad. Sch. Eng., Gunma Univ.)
アシル鎖長の異なるホスファチジルエタノールアミン二重膜の熱および圧力相転移
Thermotropic and barotropic phase transitions in bilayer membranes of phosphatidylethanolamines with varying acyl chain lengths
Masaki Goto1, Shigeru Endo2, Nobutake Tamai1, Hitoshi Matsuki1 (1Grad. Sch. of Biosci. and Bioindus., Tokushima Univ., 2Fac. of Engin.,
Tokushima Univ.)
親水性高分子により基板との距離を制御した人工膜への膜タンパク質再構成
Patterned model membrane with hydrophilic polymer brushes for the functional incorporation of membrane proteins
Fuyuko Tamura1, Yasushi Tanimoto1, Yasuhiko Iwasaki2, Fumio Hayashi3, Yuki Sudo4, Kenichi Morigaki5 (1Grad. Agri., Univ. Kobe, 2Chem.

3Pos2103Pos211

Bioeng., Univ. Kansai, 3Sci., Univ. Kobe, 4Grad. Med. Dent. Pharm., Univ. Okayama, 5Biosignal., Univ. Kobe)
バクテリオロドプシン球殻構造体の形成機構
A mechanistic insight into the formation of bacteriorhodopsin vesicle
Daisuke Yamamoto, Risa Mutoh (Fac. Sci. Fukuoka Univ.)
ホスホリパーゼ Cβ １の C 末端には脂質膜のチューブ形成能がある
Membrane tubulation ability of phospholipase Cβ1 C terminal domains
Takehiko Inaba1, Takuma Kishimoto2, Motoshide Murate1, Takuya Tajima1,3, Mitsuhiro Abe1, Asami Makino1, Nario Tomishige1, Reiko

3Pos2123Pos213

3Pos214Ishitsuka1, Yasuo Ikeda3, Shinji Takeoka3, Toshihide Kobayashi1,4 (1RIKEN, 2Kyorin Univ. Sch. Medicine, 3Waseda Univ., 4UMR 7213 CNRS,
University of Strasbourg)
巨大分子系シミュレーションに向けた粗視化脂質モデルの開発
Developing a coarse-grained model of lipid for large molecular simulations
Suguru Kato, Shoji Takada (Kyoto University)
アルギニンペプチドの膜透過を促進する両親媒性ペプチドによる脂質パッキングの変化
Alteration of Lipid Packing State by Amphipathic Peptides Promoting Membrane Penetration of Octaarginine
Tomo Murayama, Shiroh Futaki (ICR, Kyoto Univ.)
FTIR-ATR のプリズム上に作製した皮膚角層モデル膜への物質透過解析
Permeation of substances into stratum corneum model membranes prepared directly on FTIR-ATR prism
Kohei Oka, Satoru Kato (Grad. Sch. Sci. & Tech., Univ. Kwansei Gakuin)

神経回路･情報処理 / Neuronal circuit & Information processing
3Pos227

3Pos228

神経振動活動のシナプス入力による位相依存的な調節
Phase-dependent modulation of neural oscillations by synaptic inputs
Satoshi Watanabe1, Moritoshi Hirono2 (1NCNP, 2Grad. Sch. Brain Sci., Doshisha Univ.)
線虫の whole-brain イメージングデータに関する位相同期解析
Phase synchronization analysis of whole-brain imaging data of C. elegans
Yuishi Iwasaki1,7, Takayuki Teramoto2,7, Terumasa Tokunaga3,7, Osamu Hirose4,7, Yu Toyoshima5,7, Ryo Yoshida6,7, Yuichi Iino5,7, Takeshi
Ishihara2,7 (1Fac. Eng., Ibaraki Univ., 2Grad. Sch. Sci., Kyushu Univ., 3Grad. Sch. Comp. Sci. and Sys. Eng., Kyushu Institute Tech., 4Institute. Sci.

3Pos229and Eng., Kanazawa Univ., 5Grad. Sch. Sci., Univ. Tokyo, 6Institute Stat. Math., 7JST, CREST)
神経細胞から伸長する神経突起の特性のオンチップ１細胞解析
Minimum Requirements of Microchannel Patterns for Building of Stable Neuronal Circuits in On-chip Cell Network Assay
Takahito Kikuchi1, Hideyuki Terazono2, Kenji Matsuura2, Akihiro Hattori2, Masao Odaka2, Kenji Yasuda1 (1Dept. Physics, Waseda Univ.,
2WASEDA Biosci. Res. Inst. Singapore (WABIOS), Waseda Univ.)

3Pos230

フェムト秒レーザー光刺激による神経回路網の誘発応答特性
Spatio-temporal activity pattern in neuronal network evaluated by femtosecond laser-induced stimulation
Yuji Fujioka1,2, Yuta Nakagawa1,2, Suguru N. Kudoh2, Takahisa Taguchi3, Chie Hosokawa1 (1Biomed. Res. Inst., AIST, 2Sch. Sci. & Tech., Kwansei
Gakuin Univ., 3CiNet, NICT)– S133 –3Pos231

3Pos232

3Pos233

3Pos234

培養神経回路網におけるネットワークグラフ構造の培養日数依存的変化
Developmental changes of graph structures in cultured neurons -the analysis with functional-connection map
Nanami Hirata, Wataru Minoshima, Hidekatsu Ito, Suguru Kudoh (Department of Human System Interaction, School of Science and Technology,
Kwansei Gakuin University)
エピカテキンは、ヨーロッパモノアラガイの呼吸行動の中枢リズム発生器である RPeD1 ニューロンの興奮性を変化させる
Epicatechin alters the electrophysiological activity of RPeD1 in the pond snail, Lymnaea
Yoshimasa Komatsuzaki1, Minoru Saito2, Ken Lukowiak3 (1Dept. Phys., CST, Nihon Univ., 2Grad. Sch. Integ. Basic Sci., Nihon Univ., 3Hotchkiss
Brain Inst., Cumming Sch. of Med., Univ. of Calgary)
Increasing reproducibility of activity patterns in nenural network during culture days
Takumi Okada, Wataru Minoshima, Hidekatsu Ito, Suguru Kudoh (Dept. of Human System Interaction, School of Sci. and Tech., Kwansei Gakuin
University,)
超高感度な匂い識別は、受容体‐G 蛋白質の初期一過性相互作用と前梨状皮質振動性応答に部分的に支配されるSupersensitive odor discrimination is controlled in part by initial interactions of receptor-G-protein and cortical oscillatory responses
Takaaki Sato1, Reiko Kobayakawa2, Ko Kobayakawa2, Makoto Emura3, Shigeyoshi Itohara4, Takashi Kawasaki1, Riichi Kajiwara5, Ichiro
Takashima1, Toshio Iijima6, Akio Tsuboi7, Hiroyoshi Matsumura8 (1AIST, 2Kansai Med. Univ., 3Takasago Internat'l. Corp., 4BSI., RIKEN, 5Sch. Sci.

3Pos235& Technol., Meiji Univ., 6Grad. Sch. Life Sci., Tohoku Univ., 7Nara Med. Univ., 8Ritsumeikan Univ.)
定量的マンガン造影 MRI による全脳神経活動計測
Whole brain activity mapping using quantitative activation-induced manganese-enhanced MRI
Makoto Osanai1,2, Satomi Kikuta1,3, Hajime Tamura1, Noriyasu Homma1,2 (1Tohoku Univ. Grad. Sch. Med., 2Grad. Sch. Bio Med. Eng, Tohoku
Univ., 3Research Fellow, JSPS)

行動 / Behavior
3Pos236
3Pos237

3Pos238

Foraging behavior of Caenorhabditis elegans
Chien Jung Lo, Mao Ting Cheng (Dept. of Physics, NCU, Taiwan)
2 次元系および 3 次元系における細胞の自発運動動態
Spontaneous cell migration dynamics in 2D and 3D environment
Hiroaki Takagi (Dept. of Phys., Nara Med. Univ.)
活動量と睡眠との関連解析
Association between physical activity and sleep efficiency
Ikuko Motoike1,2, Atsushi Koike1, Akihiro Karashima1,3, Mitsuyuki Nakao1 (1Grad. Sch. Info. Sci., Tohoku Univ., 2Medical Megabank Org.,
Tohoku Univ., 3Grad. Sch. Eng., Tohoku Inst. Tech.)

光生物：視覚･光受容 / Photobiology: Vision & Photoreception
3Pos239

3Pos240

光誘起チャネルロドプシンに関する理論研究
Theoretical study on molecular mechanism of photo-induced gate opening of channelrhodopsin
Cheng Cheng1, Motoshi Kamiya1, Norio Yoshida2, Shigehiko Hayashi1 (1Kyoto Univ., 2Kyushu Univ.)
古細菌型 TSA モチーフ配列をもつ真正細菌由来ハライドイオンポンプロドプシンの光反応解析
Cl--pumping Photoreaction of a Bacterial Halide-ion Pumping Rhodopsin with an Archaeal-type TSA motif
Takashi Tsukamoto1, Susumu Yoshizawa2, Takashi Kikukawa3, Makoto Demura3, Yuki Sudo1 (1Grad. Sch. Med. Dent. & Pharm. Sci., Okayama

3Pos2413Pos2423Pos243

Univ., 2AORI, Univ. of Tokyo, 3Fac. Adv. Life Sci., Hokkaido Univ.)
Mastigocladopsis repens halorhodopsin のフォトサイクルの解析と TSD モチーフの役割の解明
Photocycle of Mastigocladopsis repens halorhodopsin and the role of its TSD motif
Takatoshi Hasemi, Takashi Kikukawa, Tomoyasu Aizawa, Naoki Kamo, Makoto Demura (Grad. Sch. Life Sci., Hokkaido Univ.)
ハロロドプシンの陰イオン輸送サイクルにおけるレチナール色素の異性化
Isomerization of the retinal chromophore during the anion pumping cycle of halorhodopsin
Tsutomu Kouyama, Hiroki Kubo, Siu Kit Chan, Kousuke Maki (Dept. Physics, Graduate School of Science, Nagoya University)
X 線小角散乱法を用いたシロイヌナズナ phototropin1 と変異体の構造及び機能研究
Structural and functional study of Arabidopsis phototropin1 and its mutants by using small-angle X-ray scattering
Mao Oide1,2, Koji Okajima1,2, Sachiko Kashojiya2,3, Yuki Takayama1,2, Tomotaka Oroguchi1,2, Takaaki Hikima2, Masaki Yamamoto2, Masayoshi

3Pos244

Nakasako1,2 (1Grad. Sci. Tech., Keio Univ., 2RIKEN SPring-8 Center, 3Dept. of Biol. Sci., Osaka Pref. Univ.)
Chromophore conformation in active site of orange carotenoid protein studied by Raman optical activity spectroscopy

3Pos245

Tomotsumi Fujisawa1, Masashi Unno1, Ryan Leverenz2, Cheryl Kerfeld2 (1Saga Univ., 2Michigan State Univ.)
Functional characterization of a microbial rhodopsin from the marine eubacterium Rubricoccus marinus SG-29

3Pos246

Saki Inoue1, Susumu Yoshizawa2, Takashi Tsukamoto1,3, Yuki Sudo1,3 (1Fac. Pharm. Sci., Okayama Univ., 2AORI, Univ. of Tokyo, 3Grad. Sch.
Med. Dent. & Pharm. Sci., Okayama Univ.)
Signaling kinetics of Cyanabacterial phytochrome (Cph1) studied by the transient grating method
Kimitoshi Takeda, Masahide Terazima (Department of chemistry, Kyoto University)– S134 –3Pos247

In situ 光照射固体 NMR によるセンサリーロドプシン II の光中間体の解析
Characterization of photo intermediates in sensory rhodopsin II as revealed by in-situ photo-irradiation solid-state NMR
Yoshiteru Makino1, Izuru Kawamura1, Takashi Okitsu2, Akimori Wada2, Yuki Sudo3, Naoki Kamo4, Akira Naito1, Kazuyoshi Ueda1 (1Grad. Sch.

3Pos248

Eng., Yokohama Natl. Univ., 2Kobe Pharm. Univ., 3Grad. Sch. Med. Dent. Pharm., Okayama Univ., 4Grad. Sch. Life Sci., Hokkaido Univ.)
異なる生物種におけるフォトトロピン光反応の多様性
Diversity of photoreaction of phototropins among different organisms
Yusuke Nakasone1, Koji Okajima4, Kenichi Hitomi3, John Christie3, Satoru Tokutomi2, Masahide Terazima1 (1Kyoto University, 2Osaka Prefecture

3Pos249

3Pos250

University, 3Scripps research institute, 4Keio University)
EPR 法による Photozipper 反応過程の解析
Reaction mechanism in Photozipper monitored by Electron Paramagntic Resonance
Kouhei Ozeki1, Hiroki Nagashima1, Osamu Hisatomi2, Hiroyuki Mino1 (1Grad. Sch. Sci., Nagoya Univ., 2Grad. Sch. Sci., Osaka Univ.)
Substrate recognition of the (6-4)photolyase
Yuma Terai1, Takahiro Yumiba1, Tomoko Ishikawa2, Takeshi Todo2, Junpei Yamamoto1, Shigenori Iwai1 (1Grad. Sch. Eng. Sci., Osaka Univ.,
2Grad. Sch. Med., Osaka Univ.)

3Pos251(6-4)光回復酵素による逐次的 2 光子 DNA 修復における逆電子移動の観測
Monitoring of the back electron transfer in the successive two-photons DNA repair by the (6-4) photolyase
Junpei Yamamoto1, Kohei Shimizu1, Shigenori Iwai1, Klaus Brettel2 (1Grad. Sch. Eng. Sci., Osaka Univ., 2CEA Saclay, France)

光生物：光合成 / Photobiology: Photosynthesis
3Pos252

イチョウの葉の微量色素分析
Precise pigment analysis of ginkgo leaves
Yuhta Isei1, Katsuhiro Wada1, Tadashi Watanabe2, Norio Tanaka3, Masami Kobayashi1 (1Divi. Materials Sci., Fac. Pure and Applied Sci., Univ.

3Pos253

Tsukuba, 2Res. Center Math and Sci. Edu., Org. Adv. Edu., Tokyo University of Science, 3Tsukuba Botanical Garden)
新奇クロロフィルを持つシアノバクテリアより光化学系 II 標品の単離精製とエネルギー移動機構の解析
Analysis of energy transfer system of photosystem II complexes isolated from new chlorophyll containing cyanobacterium
Toshiyuki Shinoda1, Daisuke Nii1, Seiji Akimoto2,3, Tatsuya Tomo1,4 (1Grad. Sch. Sci., Tokyo Univ. of Sci., 2Molecular Photoscience Research

3Pos254

Center, Kobe Univ., 3JST CREST, 4JST PRESTO)
Aggregation of chlorophylls d and f in n-hexane
Katsuhiro Wada1, Terumitsu Kanjoh1, Yuhta Isei1, Yutaka Hanawa2, Yoshihiro Shiraiwa2, Masataka Nakazato3, Hideaki Miyashita4, Masami
Kobayashi1 (1Div. Materials Sci., Fac. Pure Applied Sci., Univ. Tsukuba, 2Fac. Life Environ. Sci., Univ. Tsukuba, 3Chlorophyll Research Institute3Pos255

3Pos256

3Pos257

Co., Ltd, 4Graduate School Human Environ. Studies, Kyoto Univ.)
緑藻ミル糸状体における培養時光強度に依存したカロテノイドの蓄積
Extra accumulation of carotenoids upon intense irradiation during culture of a siphonous green algae, Codium fragile
Kentaro Fujiwara1, Ritsuko Fuji1,2 (1Grad. Sch. Sci., Osaka City Univ., 2OCARINA, Osaka City Univ.)
チラコイド膜での光還元に対する共溶媒の効果
Effect of co-solvents on photo-reduction in thylakoid membranes
Kuniyuki Hatori, Yuko Kokaji, Tomoyuki Toyama (Dept. Bio-Systems, Yamagata Univ.)
Identity of chlorophyll e
Yuhta Sorimachi1, Taku Kaitani1, Masataka Nakazato2, Hideaki Miyashita3, Masami Kobayashi1 (1Div. Materials Sci. Pure and Applied Sci. Univ.,
2Chlorophyll Res. Inst., 3Graduate School of Human and Environ. Sci., Univ.)

3Pos258

Solubility and stability of chlorophylls in algal oil
Terumitsu Kanjoh1, Mikihide Demura2, Masaki Yoshida2, Makoto Watanabe2, Masataka Nakazato3, Masami Kobayashi1 (1Div. Materials Sci.,

3Pos259
3Pos260

3Pos261

Fac. Pure Appl. Sci., Univ. Tsukuba, 2Fac. Life Environ. Sci., Univ. Tsukuba, 3Chl. Tes. Inst.)
Complex formation between carbon nanomaterials and photosystem complexes
Shota Tanaka, Tatsuya Tomo (Grad. Sch. Sci., Tokyo Univ. of Sci.)
緑化途上トウモロコシ生葉の極低温顕微分光による光合成タンパク質前駆体の蛍光スペクトル同定Spectral identification of late precursors to photosynthetic proteins by cryogenic microscopy of greening etiolated Zea mays leaves
Hirotomo Nagasawa, Tomofumi Chiba, Yutaka Shibata (Graduate School of Science, Tohoku University)
北海道で採取した紅色非硫黄細菌による酢酸塩からの光水素生成
Phototrophic hydrogen production from acetate by purple non-sulfur bacteria from rivers in Hokkaido
Mayoka Kanoh1, Kazuma Tazawa1, Seigo Kumakura2, Masahiro Hibino1,2 (1Div. Sustain. Enviro. Eng., Muroran Inst. Tech., 2Dept. Appl. Sci.,
Muroran Inst. Tech.)

放射線生物学･活性酸素 / Radiobiology & Active oxygen
3Pos262

低線量或いは高線量 X 線被ばく後のマウス肝臓におけるメタボローム解析
Metabolome analyses in livers of mice exposed to low or high-dose X-ray-irradiation
Tetsuo Nakajima1, Guillaume Vares2, Yasuharu Ninomiya1, Bing Wang1, Takanori Katsube1, Kaoru Tanaka1, Cuihua Liu1, Hirokazu Hirakawa1,
Kouichi Maruyama1, Akira Fujimori1, Mitsuru Nenoi1 (1Natl. Inst. Radiol. Sci., QST., 2OIST)– S135 –3Pos263

3Pos264

3Pos265

ビタミンＤによって誘発される単球の分化と酸化ストレスの関係
Relations of oxidative stress and monocytic differentiation induced by vitamin D3
Naoya Matsunaga, Kiyotaka Murakami, Wakako Hiraoka (Dept. Phys., Grad. Sch. Sci. & Tech., Meiji Univ.)
プリオンオクタペプチドと二価金属錯体結合によってひきおこされるレドックス不均衡
Redox imbalance induced by coordination of divalent metals in octarepeat region of human PrP
Shinnosuke Kondo, Wakako Hiraoka (Dept. Phys., Grad. Sch. Sci. & tech., Meiji Univ.)
超音波に誘発される CMNB-ケージ基と脂肪酸の分解
Ultrasound-induced scission of CMNB-caged moiety and fatty acids
Kengo Takei1, Takuya Wada1, Asuka Kato1, Masato Mutoh2, Wakako Hiraoka1 (1Dept. Phys., Grad. Sch. Sci. & Tech., Meiji Univ., 2Dept. Mater.
& Human Env. Sci., Shonan Inst. of Tech.)

ゲノム生物学：ゲノム機能 / Genome biology: Genome function
3Pos266

一分子観察における DNA 二重鎖の光切断に対する PEG 保護作用
Protective effect of PEG against DNA double-strand breaks caused by photo irradiation through single molecule observation
Moe Usui, Yuko Yoshikawa, Kenichi Yoshikawa (Facul. Life Med. Sci., Doshisha Univ.)

バイオインフォマティクス：機能ゲノミクス / Bioinformatics: Functional genomics
3Pos267

3Pos268
3Pos269

機械学習を用いた機能未知スプライシングアイソフォームの機能性推定
Evaluation of functionality of uncharacterized splicing isoforms using machine learning techniques
Pramote Teerasetmanakul, Masafumi Shionyu (Grad. Sch. Bio-Sci., Nagahama Inst. Bio-Sci. Tech.)Predicting protein-protein interactions using sequence homology and machine learning methods
Yifan Tang, Wei Cao, Tohru Terada, Kazuya Sumikoshi, Shugo Nakamura, Kentaro Shimizu (Grad. Sch. Agr., Univ. Tokyo)
クロマチン構造形成における単純反復配列の機能的役割
Functional Roles of Simple Repeat Sequences in Chromatin Conformations
Takeru Kameda1, Atsushi Ikegaya1, Takeshi Sugawara2, Naoaki Sakamoto1,2, Akinori Awazu1,2 (1Dept. of Mathematical and Life Sciences,3Pos270

3Pos271

3Pos272

Hiroshima University, 2Research Center for the Mathematics on Chromatin Live Dynamics)
翻訳伸長因子 1A の配列情報に基づいた機能分岐に関わる重要な残基の予測
Prediction of key residues involving functional divergence based on sequence information of translation elongation factor 1A
Yosuke Kondo, Satoru Miyazaki (Fac. Pharm., Tokyo Univ. Sci.)
天然変性領域の機能部位：Protean Segments が効果的に相互作用できる理由
Interface property of protean segments: intrinsically disordered regions that undergo disorder-to-order transitions upon binding
Divya Shaji, Takayuki Amemiya, Ryotaro Koike, Motonori Ota (Grad. Sch. Inf. Sci., Nagoya U.)
Immuno-Navigator: a co-expression database for cell type-specific network inference in the immune system
Alexis Vandenbon (IFReC, Osaka University)

バイオインフォマティクス：比較ゲノミクス / Bioinformatics: Comparative genomics
3Pos273

光回復酵素／クリプトクロムファミリーの機能発現に重要なアミノ酸残基部位の探索
In search for amino acid positions that determine the molecular function of photolyase/cryptochrome family
Daichi Yamada1, Kei Yura1,2 (1Cent. Info. Biol., Ochanimizu Univ., 2NIG)

3Pos274

Refining the performance of k-mer count similarity prediction and examining use of different scoring matrix for better pairwise alignment
Kazunori Yamada, Kengo Kinoshita (Tohoku University)

数理生物学 / Mathematical biology
3Pos275

3Pos276

緑藻の走光性と多細胞性の関係
Relation between phototaxis and multicellularity of green algae
Keisuke Yamada, Yoshihiro Murayama (Tokyo Univ. of Agri. and Tech.)
染色体の凝縮が染色体の構築や分離に与える影響
Effects of chromatin condensation on chromosome construction and segregation
Yuji Sakai1,2, Masashi Tachikawa1, Atsushi Machizuki1, Kazuhisa Kinoshita3, Tatsuya Hirano3 (1RIKEN, Theoretical Biology Laboratory, 2RIKEN,

3Pos277

3Pos278

iTHES, 3RIKEN, Chromosome Dynamics Laboratory)
クロマチン動態とコンタクトマップの関係を深く理解するための数理的研究
A mathematical study for deep understanding of relationship between chromatin dynamics and contact map
Masaki Nakagawa (RcMcD, Hiroshima Univ.)
Enhancement of sampling space in multivariate analysis of experimental big data in various biological sciences
Jiyoung Kang1, Kazuhiko Yamasaki2, Masaru Tateno1 (1Univ. of Hyogo, 2Biomed. Res. Ins. AIST)– S136 –3Pos279

血糖値調節におけるインスリン・C ペプチドの血中動態の数理モデルを用いた解析
Mathematical model analysis of blood glucose regulation with insulin and C-peptide
Kaoru Ohashi1, Masashi Fujii1, Shinsuke Uda1, Hisako Komada2, Kazuhiko Sakaguchi2, Wataru Ogawa2, Shinya Kuroda1 (1Grad. Sch. Sci., Univ.

3Pos280

3Pos2813Pos282

3Pos283

3Pos284

Tokyo, 2Grad. Sch. Med., Univ. Kobe)
生命科学研究用の特化型シミュレータ群の具現化
Implementation of a group of special-purpose simulators for life science researches
Hideto Katsuma1,2, Jun Takayama2, Yukako Tohsato2, Koji Kyoda2, Shuichi Onami1,2 (1Grad. Sch. System Inform., Kobe Univ., 2Lab. Dev Dyn.,
RIKEN QBiC)
遺伝子発現量制御メカニズムのタイプ分類と遺伝子機能の関係
Relationship between regulatory pattern of gene expression level and gene function
Masayo Inoue, Katsuhisa Horimoto (molprof, AIST)T 細胞の胸腺における細胞数のダイナミクスのモデリングと推定
Modeling and inferring dynamics of T cell population in thymus
Kazumasa Kaneko1, Ryo Yokota2, Taishin Akiyama3, Tetsuya Kobayashi2 (1Grad. Sch. Eng., Univ. Tokyo, 2Inst. Ind. Sci., Univ. Tokyo, 3Inst. Med.
Sci., Univ. Tokyo)
触媒反応系から成る細胞のトレードオフ
Trade-off in a protocell model with catalytic reactions
Atsushi Kamimura, Kunihiko Kaneko (The University of Tokyo)
Spine における Small-volume effect: Robust, Sensitive, Efficient な情報伝達のメカニズム
Small-volume effect enables robust, sensitive and efficient information transfer in the spine
Masashi Fujii1, Kaoru Ohashi1, Yasuaki Karasawa2, Minori Hikichi1, Shinya Kuroda1 (1Dept. Biol. Sci., Grad. Sch. of Sci., Univ. of Tokyo, 2Dept.
Neurol., Grad. Sch. of Med., Univ. of Tokyo)

非平衡･生体リズム / Nonequilibrium state & Biological rhythm
3Pos285

Curvature-driven splitting of a planar traveling wave
Kazuya Horibe1, Ken-ichi Hironaka2,3,4, Katsuyoshi Matsushita2, Koichi Fujimoto2 (1Grad. Sch. Info, Univ. Osaka, 2Grad. Sch. Sci., Univ. Osaka,
3CDB, Inst., Riken, 4JSPS Research Fellow(PD))

3Pos286

Quantification of dynamic mechano-response of myoblast using stimulus responsive matrix
Marcel Hoerning1, Masaki Nakahata2, Akihisa Yamamoto1, Mariam Veschgini3, Stefan Kaufmann3, Yoshinori Takashima2, Akira Harada2,4,

3Pos287

Motomu Tanaka1,3 (1iCeMS, Kyoto University, 2Osaka University, 3Heidelberg University, 4ImPACT)
再生ヒドラにおける形状ダイナミクスと対称性の破れの定量化
Quantification of Morphological Dynamics and Symmetry Break in Regenerating Hydra Tissues
Ryo Suzuki1, Mariam Veschgini2, Thomas W. Holstein3, Motomu Tanaka1,2 (1iCeMS, Kyoto University, 2Institute of Physical Chemistry, University

3Pos288
3Pos289

of Heidelberg, 3Centre for Organismal Studies, University of Heidelberg)
2D swarming bacteria
Chien Jung Lo, Ching Yuan Lin (Dept. of Physics, NCU, Taiwan)
聴覚刺激によって引き起こされる脳波の引き込み現象と確率共鳴
Auditory entrainment and stochastic resonance in EEGs
Minoru Saito1,2, Shogo Kawamoto2, Yuuta Hamasaki1, Ken Saito3, Tetsuya Yamamoto4 (1College of Humanities and Sciences, Nihon University,
2Graduate School of Integrated Basic Sciences, Nihon University, 3College of Science and Technology, Nihon University, 4Tokyo Metropolitan
College of Industrial Technology)

バイオイメージング / Bioimaging
3Pos290

Observation of conformational dynamics of FliI by HS-AFM
Kei Adachi1, Jun-ichi Kishikawa3, Hiroyuki Terashima2, Takayuki Uchihashi1, Katsumi Imada2, Ken Yokoyama3, Toshio Ando1 (1Coll. Sci. &3Pos291

Eng., Kanazawa Univ., 2Grad. Sch. Sci., Osaka Univ., 3Facul. Biosci., Kyoto Sangyo Univ.)
高速原子間力顕微鏡と光ピンセットの複合システム
Combined system of high speed atomic force microscopy(HS-AFM) and optical tweezers
Shin’nosuke Yamanaka1, Akane Goto1, Hiroki Watanabe2, Takayuki Uchihashi1,3, Toshio Ando1,3 (1Grad. Sch. Sci., Kanazawa Univ., 2RIBM Co.,

3Pos292

Ltd., 3Bio-AFM FRC., Kanazawa Univ.)
脂質膜の曲率に依存したタンパク質-脂質膜の相互作用の直接観察のための高速 AFM 用基板の開発
Development of HS-AFM substrate for observation between proteins and lipid membrane depending on the physical shape of lipid
membrane
Takahiro Toyoda1, Shin’nosuke Yamanaka1, Akane Goto1, Hiroki Watanabe2, Shunsuke Shozui1, Mikihiro Shibata1,3, Takayuki Uchihashi1,3

3Pos293(1Dept. of phys., Kanazawa Univ., 2RIBM Co., Ltd.,, 3Bio-AFM FRC., Kanazawa Univ.)
長時間１蛍光分子追跡法による接着班分子の動的リクルートの解明
Transient recruitment of focal adhesion molecules revealed by super-long single molecule tracking
Taka-aki Tsunoyama1, Kenichi G.N. Suzuki2,3, Takahiro K. Fujiwara2, Akihiro Kusumi1,2,4 (1OIST, 2Inst. Integrated Cell-Material Sciences (WPIiCeMS), Kyoto Univ., 3NCBS/inStem, India, 4Inst. Frontier Medical Sciences, Kyoto Univ.)– S137 –3Pos294

個々の酵母細胞内 ATP 濃度の可視化により明らかになった、酸化ストレス下における細胞内 ATP 濃度の低下
In vivo imaging of cytoplasmic ATP in living yeast cells reveals a profound effect of oxidative stress on ATP level
Masak Takaine1,2, Hiromi Imamura3, Satoshi Yoshida1,2 (1Gunma Univ. Initiative for Adv. Res., 2Gunma Univ. Inst. for Mol. and Cell. Regulation,
3Lab. of Funct. Biol., Grad. Sch. of Biostudies, Kyoto Univ.)

3Pos295

3Pos296

3Pos297

3Pos298

PDLIM2 の相互作用タンパク質 MKRN2 は、NF-κB の p65 サブユニットに対する新規ユビキチン E3 リガーゼとして機能する
PDLIM2-interacting protein MKRN2 functions as a novel E3 ligase for p65 subunit of NF-κB
Chanyoung Shin1,2, Yuma Ito1, Makio Tokunaga1, Takashi Tanaka2, Kumiko Sakata-Sogawa1 (1Sch. Life Sci. Tech., Tokyo Inst. Tech., 2IMS,RIKEN)
リポ多糖刺激における炎症抑制タンパク質 PDLIM2 活性化の生細胞イメージング定量解析
Live cell imaging and quantitative analysis of anti-inflammatory protein PDLIM2 activation upon LPS stimulation
Shota Ichikawa1, Yuma Ito1, Takashi Tanaka2, Makio Tokunaga1, Kumiko Sakata-Sogawa1 (1Sch. Life Sci. Tech., Tokyo Inst. Tech., 2IMS-RCAI,RIKEN)mRNA の一分子追跡によるストレス顆粒形成初期機構の調査
Investigating initiation mechanism of stress granule formation by tracking single mRNA particles
Masamichi Imaseki1, Ko Sugawara1, Kohki Okabe1,2, Takashi Funatsu1 (1Grad. Sch. Pharm Sci., Univ. of Tokyo, 2JST, PRESTO)
高速近接場光学顕微鏡の開発
Development of high-speed near-field optical microscopy
Takayuki Umakoshi1, Shingo Fukuda2, Takayuki Uchihashi1,2,3, Toshio Ando1,2,3 (1Bio-AFM FRC, Kanazawa Univ., 2Coll. Sci. & Eng., Kanazawa

3Pos299

Univ., 3CREST-JST)
転写伸長制御に関わるタンパク質動態のイメージング定量解析
Quantitative image analysis of dynamics of promoter-proximal pausing related proteins

3Pos300

Shinnosuke Kunimi, Yuma Ito, Yuki Yamaguchi, Kumiko Sakata-Sogawa, Makio Tokunaga (Sch. Life Sci. Tech., Tokyo Inst. Tech.)
大気圧電子顕微鏡を用いた分泌腺組織の水中観察
Secretory glands imaged in aqueous solution by atmospheric scanning electron microscopy (ASEM)
Toshiko Yamazawa1, Naotoshi Nakamura2, Mari Sato3, Chikara Sato3 (1Dept. Mol. Physiol., Jikei Univ. Sch. Med., 2Dept. Statistical Genetics,

3Pos301

3Pos302

Kyoto Univ., 3Biomed. Res. Inst., AIST)
ベイズ推定を用いた透過型電子顕微鏡画像の CTF 補正の自動化
Development of automated CTF correction of transmission electron microscopic images using the Bayesian estimation
Koji Hisanaga, Takuo Yasunaga (Kyushu Institute of Technology Graduate School of Computer Science and System Engineering)
様々な生物種の温度測定に利用でき且つ速い温度変化を測定可能な蛍光性温度プローブタンパク質
Genetically encoded ratiometric fluorescent thermometer with wide temperature range and rapid response
Masahiro Nakano1, Yoshiyuki Arai1, Ippei Kotera2, Kohki Okabe3,4, Yasuhiro Kamei5, Takeharu Nagai1 (1ISIR, Osaka Univ., 2RIES, Hokkaido

3Pos303

3Pos304
3Pos305

Univ., 3Grad. Sch. Pharma., Univ. Tokyo, 4JST, PRESTO, 5NIBB)
新規誘電率顕微鏡(SE-ADM)による生きたそのままの細胞の液中ナノスケール観察
Nanoscale observation of intact living cells in a medium with low radiation damage using scanning electron-assisted dielectric microscopy
Tomoko Okada, Toshihiko Ogura (Advanced Industrial Science and Technology (AIST), Biomedical Research Institute)
Improvement of photostability of fluorescent dyes by using lanthanide ions
Takuma Imoto1, Shin Mizukami2, Kazuya Kikuchi1,3 (1Grad. Sch. Eng, Osaka Univ., 2IMRAM, Tohoku Univ., 3IFReC)
Optical measurement of diffusion and pH in nanopores of protein crystals
Kazuo Mori, Bernd Kuhn (OIST)

バイオエンジニアリング / Bioengineering
3Pos306

3Pos307

3Pos308

3Pos309

アポフェリチンを用いた複合(MRI 造影・発光)希土類ナノ粒子の作製
Synthesis of rare earth hybrid nanoparticles in the apoferritin cavity
Keita Kontani, Hideyuki Yoshimura (Meiji University)
標的遺伝子高感度検出に向けた自己組織化単分子膜修飾金ナノ粒子の分散安定化
Optimized modification of SAMs for suppression of non-specific binding gold nanoparticles for High-sensitivity Target Genetic Assay
Keiko Esashika, Takaha Mizuguchi, Toshiharu Saiki (Sci. Rech., Keio Univ.)
血中循環腫瘍細胞を測定するためのサイズ分画機能を備えた画像認識型セルソーターの開発
Development of Size Classifying Imaging Cell Sorter for Identifying of Circulating Tumor Cells
Moe Iwamura1, Masao Odaka2, Kenji Matsuura2, Akihiro Hattori2, Hideyuki Terazono2, Kenji Yasuda1 (1Dept. Physics, Waseda Univ., 2WASEDA
Biosci. Res. Inst. Singapore (WABIOS), Waseda Univ.)
血中循環腫瘍細胞を無染色で識別するためのオン・チップ高機能画像認識型細胞分取装置の開発
Development of Functional On-Chip Imaging Cell Sorter for Identification of Non-Labeled Circulating Tumor Cells
Masao Odaka1, Akihiro Hattori1, Kenji Matsuura1, Hideyuki Terazono1, Moe Iwamura2, Kenji Yasuda2 (1WASEDA Biosci. Res. Inst. Singapore

3Pos310(WABIOS), Waseda Univ., 2Dept. Physics, Waseda Univ.)
ゲル媒質中で反応拡散系によるパターン形成を行う DNA 論理ゲート
DNA logic gate performs Reaction-Diffusion Pattern formation in gel medium
Keita Abe1, Ibuki Kawamata2, Shin-ichiro M. Nomura2, Satoshi Murata2 (1Dpt. Sch. Eng., Tohoku Univ., 2Grad. Sch. Eng., Tohoku Univ.)– S138 –3Pos311

3Pos312

電極埋め込み型ナノポアの AC ゲート電位による DNA の挙動制御
DNA motion and translocation controlled by nanopore with embedded gate electrode
Naoto Sakashita, Yuta Kato, Kentaro Ishida, Toshiyuki Mitsui (Coll. of Sci. & Eng., Aoyama Gakuin Univ.)
In vitro selection of novel peptide agonists for human somatostatin receptor subtype-2 using water-in-oil microdroplets
Takashi Sakurai1, Ryo Iizuka1, Yasuyuki Nakamura2, Jun Ishii3, Akihiko Kondo2, Ayaka Iguchi4, Dong H. Yoon4, Tetsushi Sekiguchi5, Shuichi
Shoji4, Takashi Funatsu1 (1Grad. Sch. of Pharm. Sci., The Univ. of Tokyo, 2Grad. Sch. of Eng., Kobe Univ., 3Org. of Adv. Sci. and Technol., Kobe

3Pos313

Univ., 4Dept. of Nanosci. and Nanoeng., Waseda Univ., 5Res. Org. for Nano & Life Innov., Waseda Univ.)
ナノポア計測の周波数解析による複数種類の microRNA のパターン認識
Pattern Recognition for MicroRNA Expressions by using Fourier Analysis on Nanopore Sensing
Akihiro Tamotsu1, Moe Hiratani2, Masayuki Ohara2, Ryuji Kawano3 (1Tokyo Univ. of Agri. and Tech. Dept. Biotech. and Life Sci., 2Tokyo Univ. of

3Pos3143Pos315

3Pos316

3Pos317

3Pos318

3Pos319

Agri. and Tech. Dept. Biotech. and Life Sci., 3Tokyo Univ. of Agri. and Tech. Dept. Biotech. and Life Sci.)
DNA を用いたナノ粒子 3D プリンタの実現
Nanoparticle 3D Printing by DNA Bonding
Yuki Sakamoto, Shoichi Toyabe (Grad. Sch. Eng. Applied Physics, Univ. Tohoku)
DNA ナノ構造の DNA ハイドロゲルへの繰り返し電子解離／会合
Repeatable electronic dissociation/association of DNA nanostructures on DNA hydrogels
Keitel Cervantes, Ibuki Kawamata, Shin-Ichiro Nomura, Satoshi Murata (Tohoku University)
作製済みリポソームへのマイクロピペットの穿刺
Microinjection into already made liposome
Shota Sato, Shin Yoshida, Tomoyuki Kaneko (LaRC, FB, Hosei Univ.)
Quantification of intersample differences in T cell populations
Ryo Yokota1, Yuki Kaminaga2, Tetsuya Kobayashi J.1,2 (1Institute of Industrial Science, the University of Tokyo, 2School of Engineering, The
University of Tokyo)
多チャンネル局所化学刺激システムの開発
Development of the multi-channel local chemical stimulation system
Masaru Kojima1, Tatsuoya Furusawa1, Hajime Fukuoka2, Yasushi Mae1, Tatsuo Arai1 (1Grad. Sch. Eng. Sci., Osaka Univ., 2Grad. Sch. Front.
Biosci., Osaka Univ.)
多電極電位計測システムを用いた心筋細胞に対するテルフェナジンの影響
Effect of Terfenadine to cardiomyocytes on multi electrode array system
Mitsuki Maruyama, Tomoyuki Kaneko (LaCR, Hosei Univ.)– S139 –