Anti-Collagen type XVII antibody, mouse monoclonal (C34)
Product code

70-357Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

GST tagged fusion protein containing the COOH-terminal portion of human
BP180

Isotype

Mouse IgG1κ

Reactivity

Collagen type XVII/BP180/BPAG2 (Human)
C-terminal fragment (aa 1,188-1,497)

Special notes

N/A

Application

1. Western blotting: x1/1,000-5,000 (Fig.1)
2. Immunofluorescence microscopy x1/250-500 (Fig2,3)

Background

Collagen is the main structural protein in the extracellular matrix found invarious connective tissues in the body such as cartilage, bones, tendons,ligaments, and skin. It provides structural support to the extracellular space ofconnective tissues. Type XVII collagen promotes adhesion of basal keratinocytes
to epidermal basement membrane, and is the target of disease in patients withcertain inherited or acquired blistering diseases. Two forms of type XVII
collagen are found in cultured human keratinocytes: a 180-kDa full-length,transmembrane protein (known as BP180 antigen) and a recently identified
120-kDa soluble fragment that corresponds to its collagenous ectodomain.

Data LinkUniProtKB: Q9UMD9 (COHA1_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 70-357 Anti-Collagen type XVII antibody, mouse monoclonal (C34)*DJM-1lysate

Fig. 1 Western blot analysis of C34 antibody
Whole cell extract prepared from human carcinoma derived DJM-1 cells was stained with CBB andimmunoblotted with C34 antibody at 1:5000 dilution. The HRP-conjugated goat anti-mouse IgG wasused as the second antibody. This antibody detected an approximate 180 kDa band of type XVII
collagen (BP180, arrow) and 120 kDa bands of soluble fragments (asteorisks). Protein bands werevisualized using a chemiluminescent detection with EzWestLumi plus kits (ATTO, Tokyo, Japan).

Fig.2 Immunofluorescence microscopy of human skin
A frozen acetone-fixed human skin section was stained with C34 antibody (1:500 dilution). The FITCconjugated goat anti-mouse IgG was used as the second antibody. The antibody revealed the locationof type XVII collagen at the dermal-epidermal junction (arrows). E: epidermis, D: dermis. Bar = 50
m.

2 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Fig. 3 Immunofluorescence microscopy of DJM-1 cells
Methanol-fixed human carcinoma derived DJM-1 cells were stained with C34 antibody (1:500 dilution,green) and DAPI (cyan). The FITC-conjugated goat anti-mouse IgG was used as the second antibody.
The antibody detected arc- (arrowheads) or brush mark-like (arrows) patterns of hemidesmosomes.
Bar = 20 m.
Reference
1. Hirako Y., Yonemoto Y, Yamauchi T, Nishizawa Y, Kawamoto Y, Owaribe K.

Isolation of ahemidesmosome-rich fraction from a human squamous cell carcinoma cell line. Exp Cell Res., 324:172-182, 2014.
2. Hirako Y, Usukura J, Uematsu J, Hashimoto T, Kitajima Y, Owaribe K. Cleavage of BP180, a 180kDa bullous pemphigoid antigen, yields a 120-kDa collagenous extracellular polypeptide. J Biol
Chem. 273:9711-9717, 1998
Related Products
70-355 Anti-Collagen alpha (VII) antibody, mouse monoclonal

3 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/