Anti-Activated Caspase 3 (p20/p17 subunit) antibody, rabbit serum (ACP3)
Product code74-102Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.05% sodium azide

PurityRabbit antiserum

ImmunogenSynthetic peptide corresponding to the caspase 3 cleavage site, 6 aa (CGIETD)

IsotypeRabbit IgG

Reactivity

Specific to the end of the activated caspase 3 of human, mouse and rat. Theantibody does not react with the proenzyme p32.

Special notes

N/A

Application

1. Western blotting (dilution: 1/3,000-1/1,000)
2. Immunocytochemistry (dilution: 1/1,000-1/500)
3. ELISA
These applications were confirmed in the laboratory of Prof. K, Yoshikawa of
Osaka University (ref.3).

BackgroundCaspases are a family of cysteine proteases which play essential roles in
apoptosis. Among them, Caspase 3 is a frequently activated death protease,catalyzing the specific cleavage of many key cellular proteins. Caspase 3 issynthesized as an inactive 32 kDa pro-enzyme which undergo proteolytic
processing in response to apoptotic stimulation to produce the active form which
consists of the p20/p17, and p12 subunits. Caspase 3 is the predominant caspaseinvolved in the cleavage of Alzheimer amyloid precursor protein (APP), which is
associated with neuronal death in Alzheimer ‘s disease. An antibody (namedACP3) against activated caspase 3 was raised in rabbit. This antibody
recognizes the active form of human caspase 3, p20/p17 subunit but does notrecognize the proenzyme p32.

Data Link

UniProtKB P42574 (CASP3_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 74-102 Anti-Activated Caspase 3 (p20/p17 subunit) antibody, rabbit serum (ACP3)

Fig.1 Immunocytochemistry for APP, chromosomal DNA, and activated caspase 3 subunits.
Caspase 3 activation in neurons accumulating wild-type APP (ref.3). NT2 neurons (neurallydifferentiated human NT2 embryonic carcinoma cells) were infected with adenovirus vector
expressing β-galactosidase (upper panel) or APP (lower panel), fixed 48 h later, and triply stained for
the N-terminus of APP (with antibody P2-1) or β-gal (with antibody against β-gal), chromosomal DNA(Hoechst), and activated caspase 3 subunits (with antibody ACP3). Some neurons accumulating APP
are strongly immunostained with ACP3 (arrows), whereas neurons accumulating β-gal are hardly
labeled (arrowheads). β-gal APP Hoechst ACP3 β-gal or APP
References: This antibody was used in ref.3 and 4.
1. Thornberry NA and Lazebnik Y (1998) “Caspases: enemies within.” Science 281: 1312-1316 PMID:9721091
2. Uetsuki T et al (1999).”Activation of neuronal caspase-3 by intracelular accumulation of wild-type
Alzheimer precursor protein.” J Neurosci 19: 6955-6964 PMID: 10436052
3. Nishimura I et al. (2002) “Cell death induced by a caspase-cleaved transmembrane fragment of
the Alzheimer amyloid precursor protein.” Cell Death Differ. 9: 199-208 PMID: 11840170
4. Nishimura I et al. (2003) “Upregulation and antiapoptotic role of endogenous Alzheimer amyloid
precursor protein in dorsal root ganglion neurons.” Exp. Cell Res. 286: 241-251 PMID: 12749853
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