GeoMx®

Digital Spatial Profiler

nanostring.com
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

Heterogeneity Resolvedwith Specificity
Spatially resolve tissue cell populations withfunctional segementation
Understanding tissue heterogeneity is crucial to studying developmental biology, disease
pathogenesis, and response to treatment. Bridging the gap between tissue imaging and molecularprofiling technologies such as single cell anaysis, the GeoMx® Digital Spatial Profiler (DSP) allows
you to unlock novel biological insights with spatial multiomics.
Color KeyIn situ Visualization
Technology (FISH/ IHC)

123

Value

Molecular ProfilingTechnologies

4

Cell Functi

Regulation

T−Cell Fun+

Spatial–Plex–

Quantitation–Precision–Transporte+Pathogen D+Macrophag+

Antigen Pr

Chemokine

Senescenc

Compleme
Cell Cycle

B−Cell Fun
Adhesion
TLR

Cytokines

Leukocyte

Cytotoxicit

TNF Supe

Interleukin

Microglial F

2 FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

Subtype: C vs.
NormalSubtype: D vs.
NormalSubtype: B vs.
NormalSubtype: A vs.
NormalTumor.Grade: Grade ll vs.
NormalTumor.Grade: Grade l vs.
NormalBMINK Cell Fu

Introducing

GeoMx

®

Digital Spatial Profiler
GeoMx DSP seamlessly enables spatialtranscriptomics and proteomics with a workflowthat combines standard pathology andmolecular profiling with efficient data analysis.
GeoMx RNA assays enable quantitative, spatial
analysis of 100s of transcripts up to the wholetranscriptome from a single section of FFPE or
fresh frozen tissue.

HETEROGENEITY,RESOLVEDUNLOCK YOURSAMPLES WITHCONFIDENCECONSISTENTRESULTS,RELIABLEANSWERS

GeoMx Protein assays enable targeted spatial
profiling of 100s of proteins from FFPEtissue sections.
GeoMx DSP also enables Spatial Proteogenomics
by combining both Protein and RNA assays onthe same slide.

DETECT MOREWITHOUTCOMPROMISEANALYZETODAY, PUBLISHTOMORROWSTRUCTURE
DICTATESFUNCTION

FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

3

The Path is Clear
GeoMx® DSP Workflow

Using standard IHC methodologies, tissue sections are stained with a mixture of fluorescently-labeledand DNA-barcoded antibodies. Once imaging and profiling is complete, GeoMx DSP stores data fromeach region of interest (ROI) after the expression of a target is quantified.

1Sample Prep*

Fluorescent
Antibodies

2Profile

DSP Barcoded
Antibodies orRNA

Slide Mounted
TissueAny sample, FFPE or fresh
frozen, use any morphology
marker, detect RNA and/orProtein.
*Can be automated.

4 FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

Image and profile RNA andProtein with GeoMx DSP.

3Count

4Analyze

Illumina

nCounterncounter SPRINT profiler

Count barcodes on thenCounter® Analysis Systemor sequence with NGS.

Pre-defined data processing
pipelines and interactivedata analysis accelerate
biological insight.

FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

5Enabling Your Researchwith the Most Flexible
Spatial Solution
Whether you are a discovery or translational
researcher, the GeoMx® DSP is the mostflexible spatial solution designed to conformto your ever-changing research needs.

GeoMx DSP combines standard
immunofluorescent techniques with molecularprofiling technology to perform highly
multiplexed, spatially resolved experiments.

TissueAtlasing

Biomarker
Discovery andValidation

Unbiased
Pathway
Analysis

ImmuneProfiling

6 FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

Figure 7 - Main

Locate Your Regions of Interest
Understand TissueA
Structure with Flexibilty

1Geometric Profiling

The Islet of Langherans isgeometrically profiled
2 withInsulin, glucagon and PanCK
morphology markers.

Profile with any geometricshape to characterize distinct
tissue regions.

34A 5 µm sectionof kidney depicts
the proximal convoluted tubule.
This section has been segment
profiled guided by CD10 and CD31
morphology marker staining.

Segment Profiling
Identify and profile distinct
biological compartments withina region of interest (ROI).

ROI 1Conture Profiling
Evaluate how proximity affects
biological response and thelocal microenvironment arounda central structure using
radiating ROIs.

12345

67

89ROI 2

12 3
The1 invasive
4 5 margins of two
26 7
38 9
colorectal tumor samples
are
10
45
analyzed using contour segments
6extending into the tumor andoutside the tumor into the stroma
and profiled with 1400+ RNA
probes with NGS read out.

121110Gridded Profiling

B

Perform rigorous spatial
mapping using a tunablegrid pattern.

Cell Type SpecificProfiling
Reveal the function of cell
populations guided by cell typespecific morphology markers.

ROI 1AOIROI

1 2 3 4 5 6 7 8 9 10 11 12

Gridded protein profiling ofa tonsil section, stained withmorphology
CD3, CD20 ROI 3
ROImarkers
2andPanCK.
1 2 3 4 5 6 7 8 9 10
1 2 3 4 5 6 7 8Paneth Cells from the Colon, stainedwith morphology marker 5-HT.

FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

7

Flexible, Pre-Validated Protein Contentto Fit a Range of Research NeedsGeoMx ® protein assays are modular and optimized for robust performance across a variety of sample
types. Choose your readout method and the core of your choice. For readout on the nCounter®
Analysis System, add on up to 6 modules plus as many as 10 custom targets of choice. For NGS
readout, add on as many different modules as you prefer plus up to 10 custom targets of choice.
Available content covers immunology, immuno-oncology, and neuroscience with a rapidly
growing portfolio.

Human orMouse
Protein CoreIO Drug Target
Panel

P13K/AKT SignalingPanelAlzheimer’s Pathology
Panel

Immune Activation
Status Panel

Cell Death
Panel

Alzheimer’s Pathology
Extended Panel

Immune Cell Typing
Panel

Neural Cell Typing
PanelAutophagy
Panel

Pan-Tumor
Panel

Alzheimer’s Pathology
Panel

Glial Cell Subtyping
Panel

Scan tolearn more

MAPK Signaling
Panel

Proteogenomics Meets Spatial Biology with GeoMx DSP
Simplify your spatial proteogenomic workflow with multimodal, co-detection of RNA and Protein fromthe same tissue section. Combine the GeoMx RNA Assays and GeoMx Protein Assays to gain a complete
picture of biology all the way from transcription to protein activation.

DNAGeoMx Protein Assays

GeoMx RNA Assays
RNAPROTEIN
PTranscription

Scan tolearn more

Translation

Translational
Regulation

Transcription
Regulation
Degradation

8 FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

Ub

Ub
Ub

Kinases/Phosphatases

Protein Turnover/Degradation

GeoMx® Whole Transcriptome Atlases
Spatial Resolution for Any Target
The GeoMx Whole Transcriptome Atlas (WTA) provides an unbiased, spatial view of all proteincoding genes by leveraging the power of NGS. Whether you are mapping the architecture of tissue
or exploring the regulation of morphological features, WTA delivers the highest sensitivity for spatialwhole transcriptomics on FFPE tissues.

pulldown,✔ Non-Poly-A
whole transcriptome assay✔ Complete coverage✔ Visualize morphology✔ Streamline workflows✔ Customization✔ Minimal sequencing✔ Co-detection with protein
Explore More GeoMx RNA Assays
GeoMx RNA assays include an immune pathways panel for nCounter readout that allows for profilingof up to 84 human RNAs, including probes for the Tumor Inflammation Signature (TIS) and the ability
to add up to 10 targets of interest and 2 additional controls. For higher plex spatial transcriptomics,
take advantage of NGS readout and choose from the targeted Cancer Transcriptome Atlas or design aCustom RNA Assay of up to 400 targets from any species.
SCU

SOLUTIOTOM
NS

Scan tolearn more

GeoMx™ DSPFOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

9Explore Your Data
GeoMx® Data Analysis Suite (DSPDA)DSPDA is an interactive data analysis suite that connects quantitative data to spatial context to provide aseamless experimental workflow.

Visualize your counts based onyour ROI selection

Data QC andnormalization

Visualize pathway analysis, differential
expression, heatmaps and more!

GeoScript™ Hub
NanoString has validated and released code packages to the open-source community;
Explore GeoScript Hub to see how these tools can be used to configure data analysis pipelines.

Normalization
for Protein

Dimension
Reduction

RNA Negative
Normalization

Volcano Plot

10 FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

Spatial Decon

Scan to learn
more about theGeoScript HubCell-TypeContouring

10Consistent Results, Reliable Answers
Multi-Sample Analysis and Cohort Studies Made Easy
with Unmatched Reproducibility and Scalability
Human FFPE Tonsil

Mouse Fixed Frozen Brain

Proven reproducibility
between slides, users, andinstruments with the Whole
Transcriptome Atlas.

Detect More with Sensitivity
GeoMx® DSP detects the most relevant low to high expressing genes. Detect only the targets that matterin the regions that matter.
DynamicExpression Range

Expression bins defined using
TCGA NSCLC data

Non-Small Cell Lung Cancer

Tumor Profile

Immune Profile
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

11Specifications
Category

GeoMx® DSP Instrument

GeoMx DSP Reagents

GeoMx Data Analysis
SoftwareFeatureSpecification

Sample Throughput

Up to 8 slides/day (at 12 ROIs per slide, 10 mm by 10 mm scan area)

Minimum UV Illumination Area

10 µm diameter

Resolution

20X 0.45 NA objective

Imaging Modes

Fluorescence

Imaging Channels (representative dyes)

4 fluorescent channels: (FITC/SYTO13/AF488), (CY3/AF532/PE/SYTO83), (TxRed/AF594),(CY5/AF647/Dylight 650)

Imaging channels (em center wavelength/bandpass)516/23, 564/15, 623/30, 683/30

Slide Capacity

Four 1 x 3 inch slides

On Instrument Data Storage Capacity

8TB (> 300 10 mm x 10 mm 4 channel slide images)

Long Term Data Storage

Customer-provided fileshare (local network)

ROI Definition

On-instrument or via web browser

ROI Selection

Geometric, Segmentation, Cell Type Specific Phenotype, Contouring, Gridding

Instrument Dimensions

Actual: 30" x 29" x 24"/76 cm x 73 cm x 61 cm

Instrument Weight

220 lb/100 kg

Power source

110-240 VAC, 50/60Hz, 440VA

Readout Instrument Compatability

nCounter Analysis System, Illumina NGS

Image Export

Single-channel pyramidal TIFF; monochrome or color images (JPEG, PNG, WEBP);
multicannel pyramidal, stitched OME- TIFF

Supported Analytes

Protein and RNA

Chemistry Multiplexing Platform Capabilities

Up to 96 plex for nCounter, 20K plex for NGS

Data Visualization and Analysis

Intuitive and interactive interface that automatically connects quantitative readout with spatial
information. Workflow includes QC and normalization. Visualization include clusters, heatmaps, volcano
plots, bar graphs, box plots, strip plots, scatter plots, correlation plots.

Data Export.xlsx file format for raw or calibrated data

Image Export.svg format for visualization plots

Instrument Information
ProductGeoMx DigitalSpatial Profiler

Description

Catalog Number

GeoMx Digital Spatial Profiler Analysis Instrument. Includes 1 year manufacturers warranty.

GMX-DSP-1Y

GeoMx Digital Spatial Profiler Analysis Instrument. Includes 1 year manufacturers warranty and 1 year service contract.

GMX-DSP-2Y

GeoMx Digital Spatial Profiler Analysis Instrument. Includes 1 year manufacturers warranty and 2 year service contract.

GMX-DSP-3Y

GeoMx Digital Spatial Profiler Analysis Instrument. Includes 1 year manufacturers warranty and 3 year service contract.

GMX-DSP-4Y

GeoMx Digital Spatial Profiler Analysis Instrument. Includes 1 year manufacturers warranty and 4 year service contract.

GMX-DSP-5Y

For more information, visit nanostring.com/GeoMxDSP
Bruker Spatial Biology, Inc.
530 Fairview Avenue North
Seattle, Washington 98109

T (888) 358-6266
F (206) 378-6288

nanostring.com
info@nanostring.com

Sales Contacts
United States us.sales@nanostring.com
EMEA: europe.sales@nanostring.com

Asia Pacific & Japan apac.sales@nanostring.com
Other Regions info@nanostring.com

FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
©2024 Bruker Spatial Biology. All rights reserved. NanoString, NanoString Technologies, GeoMx, the NanoString logo, and nCounter are trademarks orregistered trademarks of Bruker Spatial Biology, Inc. , in the United States and/or other countries. All other trademarks and/or service marks not owned
by Bruker Spatial Biology that appear are the property of their respective owners.
MAY 2024 MK4261