Anti-MAGE-G1 / Necdin-like 2 antibody, rabbit serum (MG1)
Product code74-114Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.05% sodium azide

PurityRabbit antiserum

ImmunogenPurified recombinant MBT-fused mouse MAGE-G1 (aa 1-279)

IsotypeRabbit IgG

Reactivity

Mouse, rat and human MAGE-G1.

Special notes

N/A

Application

1. Western blotting (1/1,000-1/300)
2. Immunoprecipitation.
3. Immunoaffinity purification
4. ELISA
Other applicacatiotons have not been tested.

Background

MAGE-G1 (melanoma-associated antigen G1, also designated necdin-like 2)gene encodes a necdin homologous protein. MAGE-G1 gene, similarly to necdin
gene, has been mapped to the region of proximal chromosome 15q in human,
which is subject to genomic imprinting and implicated in various humanneurological and mental disorders (ref.1). From this finding it is suggested thatMAGE-G1 isinvolved in brain development, and its abnormality causes

neurodevelopmental diseases, although its biochemical and functional features
remain largely unknown. MAGE-G1 has characteristics similar to those ofnecdin, which suppresses cell growth by inducing cell cycle arrest. MAGE-G1,
like necdin, targets both the transcription factor E2F1 and p75 neutrophin
receptor (p75NTR) to regulate cell viability during brain development (ref.2). An
antibody (named MG1) against mouse MAGE-G1 was raised in rabbit (ref.2).

Data Link

UniProtKB Q9CPR8 (mouse), Q96MG7 (human)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTICPROCEDURES. NOT FOR MILITARY USE.
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Data Images: 74-114 Anti-MAGE-G1 / Necdin-like 2 antibody, rabbit serum (MG1)

Fig.1 Western blotting of crude extract ofmouse embryonic cells with anti-MAGE-G1 antibody (MG1).
Sample (10 μg protein) was prepared from mouse embryonic fibroblast E 14.5.
The antibody was used at 1/300 dilution.
Numbers on the right are positions of molecular mass in kDa.

Fig.2

Western blot analysis of MAGE-G1 at different stages of neural differentiation.with thisantibody .
MAGE-G1 (G1) was analyzed by Western blotting in whole lysates of mouse P19 embryonal carcinoma
cells at different stages of neural differentiation. UN, undifferentiated P19 cells; RA, aggregated cells
treated with retinoic acid; PN, enriched postmitotic neurons.
The result revealed that P19 cells express MAGE-G1 (32 kDa) during the course of neuronaldifferentiation. The level of MAGE-G1 was the highest in retinoic acid-treated P19 cells.

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Fig.3 Immunoaffinity purification of the protein complex with anti-MAGE-G1 antibody.
Detection of endogenous complexes of MAGE-G1 with E2F1 and p75NTR proteins. The lysate fromretinoic acid-treated P19 cells was applied to immunoaffinity columns of anti-MAGE-G1 IgG ( G1IgG) and preimmune IgG (Preimmune IgG). Bound proteins were immunoblotted for E2F1, p75NTR,and MAGE-G1 (G1) with respective antibodies. MAGE-G1 endogenously forms stable complexes withE2F1 and p75NTR in differentiated P19 cells.
References: This antibody was produced and used in the following publication.
1. Kuwako K et al. (2004) “Necdin-related MAGE proteins differentially interact with the E2F1
transcription factor and the p75 neutrophin receptor.” J Biol Chem 279: 1703-1712

PMID:

14593116
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Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/