PUBLICATION LIST (Original papers)
１.

Watanabe-Takano H., Kato K., Oguri-Nakamura E., Ishii T., Kobayashi K., Murata T.,
Tsujikawa K., Miyata T., Kubota Y., Hanada Y., Nishiyama K., Watabe T., Fässler R., Ishii
H., Mochizuki N., Fukuhara S. Endothelial cells regulate alveolar morphogenesis byconstructing basement membranes acting as a scaffold for myofibroblasts. Nat. Commun.
2024 Mar 4;15(1):1622. doi: 10.1038/s41467-024-45910-y.

２.

Yamamoto K., Watanabe-Takano H., Oguri-Nakamura E., Matsuno H., Horikami D., Ishii
T. Ohashi R., Kubota Y., Nishiyama K., Murata T., Mochizuki N., Fukuhara S.* Rap1 small
GTPase is essential for maintaining pulmonary endothelial barrier function in mice.
FASEB J. 2023 Dec;37(12):e23310. doi: 10.1096/fj.202300830RR.

３.

Mizukami K., Higashiyama H., Arima Y., Ando K., Okada N., Kose K., Yamada S.,
Takeuchi J.K., Koshiba-Takeuchi K., Fukuhara S., Miyagawa-Tomita S., Kurihara H.
Coronary artery established through amniote evolution. eLife 2023; 12:e83005.
https://doi.org/10.7554/eLife.83005

４.

Yuge S, Nishiyama K., Arima Y., Hanada Y., Oguri-Nakamura E., Hanada S., Ishii T.,
Wakayama Y., Hasegawa U., Tsujita K., Yokokawa R., Miura T., Itoh T., Tsujita K.,
Mochizuki N., Fukuhara S.* Mechanical loading of intraluminal pressure mediates woundangiogenesis by regulating the TOCA family of F-BAR proteins. Nat. Commun. 2022
May 12;13(1):2594. doi: 10.1038/s41467-022-30197-8.

５.

Ando K., Tong L., Peng D., Vázquez-Liébanas E., Chiyoda H., He L., Liu J., Mochizuki
N., Fukuhara S., Grutzendler J., Betsholtz C. KCNJ8/ABCC9-containing K-ATP channel
modulates brain vascular smooth muscle development and neurovascular coupling. Dev.
Cell 2022 Jun 6;57(11):1383-1399.e7. doi: 10.1016/j.devcel.2022.04.019.

６.

Nishimura Y.**, Ishii T.**, Ando K., Yuge S., Nakajima H., Zhou W., Mochizuki N.,
Fukuhara S.* Blood flow regulates glomerular capillary formation in zebrafish pronephros.
Kidney360 April 2022, 3 (4) 700-713; DOI: https://doi.org/10.34067/KID.0005962021**Equal contribution.

７.

Sasaki Y., Higashijima Y., Suehiro J., Sugasawa T., Oguri-Nakamura E., Fukuhara S.,
Nagai N., Hirakawa Y., Wada Y., Nangaku M., Kanki Y. Lysine demethylase 2B
regulates angiogenesis via Jumonji C dependent suppression of angiogenictranscription factors. Biochem Biophys Res Commun. 2022 May 21;605:16-23. doi:
10.1016/j.bbrc.2022.03.054.

８.

Peng D., Ando K., Hußmann M., Gloger M., Skoczylas R., Mochizuki N., Betsholtz C.,
Fukuhara S., Schulte-Merker S., Lawson ND., Koltowska K. Proper migration oflymphatic endothelial cells requires survival and guidance cues from arterial mural cells.
eLife 2022 Mar 22;11:e74094. doi: 10.7554/eLife.74094.
９.

Watanabe-Takano H., Fukumoto M., Fukuhara S., Mochizuki N. Protocol for whole-mountX-gal staining combined with tissue clearing in embryo and adult mouse using CUBIC.
STAR Protoc. 3: 101127, March 18, 2022.

１０. Okasato R., Kano K., Kise R., Inoue A., Fukuhara S., Aoki J. An ATX-LPA6-Gα13-ROCKaxis shapes and maintains caudal vein plexus in Zebrafish. iScience 2021 Oct 12;24(11):103254. doi: 10.1016/j.isci.2021.103254.
１１. Watanabe-Takano H., Ochi H., Chiba A., Matsuo A., Kanai Y., Fukuhara S., Ito N., Sako
K., Miyazaki T., Tainaka K., Harada I., Sato S., Sawada Y., Minamino N., Takeda S., Ueda
H.R., Yasoda A., Mochizuki N. Mechanical load regulates bone growth via periosteal
Osteocrin. Cell Reports 2021 Jul 13;36(2):109380. doi: 10.1016/j.celrep.2021.109380.
１２. Ando K., Shih Y.-H., E. Lwaki, Grosse A, Portman D., Chiba A., Mattonet K., Gerri C.,
Stainier D.Y.R, Mochizuki N., Fukuhara S., Betsholtz C, Lawson N.D. Conserved andcontext-dependent roles for Pdgfrb signaling during zebrafish vascular mural cell
development. Dev. Biol. 2021 Nov;479: 11-22. doi: 10.1016/j.ydbio.2021.06.010. Epub
2021 Jul 24.
１３. Rho S., Oguri-Nakamura E., Ando K., Yamamoto K., Takagi Y., Fukuhara S.* Protocol for
analysis of integrin-mediated cell adhesion of lateral plate mesoderm cells isolated fromzebrafish embryos. STAR Protoc. 2021 Mar 31;2(2):100428. doi:
10.1016/j.xpro.2021.100428. eCollection 2021 Jun 18.
１４. Abdelhakim M., Dohi T., Yamato M., Takada H., Sakai A., Suzuki H., Ema M., Fukuhara
S., Ogawa R. A new model for specific visualization of skin graft neoangiogenesis using
Flt1-tdsRed BAC transgenic mice. Plast. Reconstr. Surg. 2021 Jul 1;148(1):89-99. doi:
10.1097/PRS.0000000000008039.
１５. Fukushima Y., Nishiyama K., Kataoka H., Fruttiger M. Fukuhara S., Nishida K.,
Mochizuki N., Kurihara H., Nishikawa S., Uemura A. RhoJ integrates attractive andrepulsive cues in directional migration of endothelial cells. EMBO J. 2020 Jun
17;39(12):e102930. doi: 10.15252/embj.2019102930.
１６. Kobayashi I., Kobayashi-Sun J., Hirakawa Y., Ouchi M., Yasuda K., Kamei H.,
Fukuhara S., Yamaguchi M. Dual role of Jam3b in early hematopoietic and vasculardevelopment. Development 2020 Jan 8;147(1). pii: dev181040. doi: 10.1242/dev.181040.
１７. Simmons S., Sasaki N., Umemoto E., Uchida Y., Fukuhara S., Kitazawa Y., Okudaira M.,

Inoue A., Tohya K., Aoi K., Aoki J., Mochizuki N., Matsuno K., Takeda K., Miyasaka M.,
Ishii M. High-endothelial cell-derived S1P regulates dendritic cell localization and vascular
integrity in the lymph node. eLife 2019 Oct 1;8. pii: e41239. doi: 10.7554/eLife.41239.

１８. Shin M., Nozaki N., Idrizi I., Isogai S., Ogasawara K., Ishida K., Yuge S., Roscoe B.,
Wolfe S.A., Fukuhara S., Mochizuki N, Deguchi T., Lawson N.D. Valves are a conserved
feature of the zebrafish lymphatic system. Dev. Cell 2019 Nov 4;51(3):374-386.e5. doi:
10.1016/j.devcel.2019.08.019.
１９. Rho S., Kobayashi I., Oguri-Nakamura E., Ando K., Fujiwara M., Kamimura N., Hirata H.,
Iida A., Iwai Y., Mochizuki N., Fukuhara S. (Corresponding author). Rap1b promotes
Notch signal-mediated hematopoietic stem cell development by enhancing integrinmediated cell adhesion. Dev. Cell 2019 Jun 3;49(5):681-696.e6. doi:
10.1016/j.devcel.2019.03.023.
２０. Noishiki C.**, Yuge S.**, Ando K., Wakayama Y., Mochizuki N., Ogawa R., Fukuhara S.
(Corresponding author). Live imaging of angiogenesis during cutaneous wound healing in
adult zebrafish. Angiogenesis 2019 May;22(2):341-354. doi: 10.1007/s10456-018-09660y. **Equal contribution.
２１. Ando K., Wang W., Peng D. Chiba A., Barske L., Crump J.G., Stainier D.Y.R., Lendahl U.,
Lagendijk A., Koltowska K., Hogan B.M., Fukuhara S., Mochizuki N., Betsholtz C. Periarterial specification of vascular mural cells from naïve mesenchyme requires Notch
signaling. Development 2019 Jan 25;146(2). pii: dev165589. doi: 10.1242/dev.165589.
２２. Takara K., Eino D., Ando K., Yasuda D., Naito H., Tsukada Y., Iba T., Wakabayashi T.,
Muramatsu F., Kidoya H., Fukuhara S., Mochizuki N., Ishii S., Kishima H., Takakura N.
Lysophosphatidic acid receptor 4 activation augments drug delivery in tumors bytightening endothelial cell-cell contact. Cell Reports 2017 Aug 29;20(9):2072-2086. doi:
10.1016/j.celrep.2017.07.080.
２３. Miura K., Nojiri T., Akitake Y., Ando K., Fukuhara S., Zenitani M., Kimura T., Hino J.,
Miyazato M., Hosoda H., Kangawa K. CCM2 and PAK4 act downstream of atrial
natriuretic peptide signaling to promote cell spreading. Biochem. J. 474: 1897-1918(2017). doi: 10.1042/BCJ20160841.
２４. Nakajima H., Yamamoto K., Agarwala S., Terai K., Fukui H., Fukuhara S., Ando K.,
Miyazaki T., Yokota Y., Schmelzer E., Belting H.-G., Affolter M., Lecaudey V.,
Mochizuki N. Flow-dependent endothelial YAP regulation contributes to vessel
maintenance. Dev. Cell 2017 Mar 27;40(6):523-536.e6. doi: 10.1016/j.devcel.2017.02.019.
２５. Chiba A., Watanabe-Takano H., Terai K., Fukui H., Miyazaki T., Uemura M., Hashimoto
H., Hibi M., Fukuhara S., Mochizuki N. Osteocrin, a peptide secreted from the heart andother tissues, contributes to cranial osteogenesis and chondrogenesis in zebrafish.
Development 2017 Jan 15;144(2):334-344. doi: 10.1242/dev.143354.
２６. Ando K., Fukuhara S. (Co-corresponding author), Izumi N., Nakajima H., Fukui H., Kelsh
R.N., Mochizuki N. Clarification of mural cell coverage of vascular endothelial cells by

live imaging of zebrafish. Development 2016 Apr 15;143(8):1328-39. doi:
10.1242/dev.132654.
２７. Chávez-Vargas L., Adame-García S.R., Cervantes-Villagrana R.D., Castillo-Kauil A.,
Bruystens J.G.H., Fukuhara S., Taylor S.S., Mochizuki N., Reyes-Cruz G., Vázquez-Prado
J. Protein kinase A (PKA) Type I interacts with P-Rex1, a Rac guanine nucleotide exchange
factor: Effect on PKA localization and P-Rex1 signaling. J. Biol. Chem. 291: 6182-6199(2016). doi: 10.1074/jbc.M115.712216
２８. Yokota Y., Nakajima H., Wakayama Y. Muto A., Kawakami K. Fukuhara S., Mochizuki N.
Endothelial Ca2+ oscillations reflect VEGFR signaling-regulated angiogenic capacity invivo. eLife 2015 Nov 20;4. pii: e08817. doi: 10.7554/eLife.08817.
２９. Kim J.-D., Park K.-E., Ishida J., Kako K., Hamada J., Kani S., Takeuchi M., Namiki K.,
Fukui H., Fukuhara S., Hibi M., Kobayashi M., Kanaho Y., Kasuya Y., Mochizuki N.,
Fukamizu A. PRMT8 as a phospholipase maintains dendrite formation of Purkinje cells.
Science Advances 2015 Dec 4;1(11):e1500615. doi: 10.1126/sciadv.1500615.
３０. Sugihara K., Nishiyama K., Fukuhara S., Uemura A., Arima S., Kobayashi R., Kohn-Luque
A., Mochizuki N., Suda T., Ogawa H., Kurihara H. Autonomy and non-autonomy ofangiogenic cell movements revealed by experiment-driven mathematical modeling. Cell
Reports 2015 Dec 1;13(9):1814-27. doi: 10.1016/j.celrep.2015.10.051.
３１. Hongu T., Funakoshi Y., Fukuhara S., Suzuki T., Sakimoto S., Takakura N., Ema M.,
Takahashi S., Itoh S., Kato M., Hasegawa H., Mochizuki N., Kanaho Y. Arf6 regulates
tumor angiogenesis and growth through HGF-induced endothelial 1 integrin recycling.
Nat. Commun. 2015 Aug 4;6:7925. doi: 10.1038/ncomms8925.
３２. Vanhollebeke B., Stone O., Bostaille N., Cho C., Zhou Y., Maquet E., Gauquier A.,
Cabochette P., Fukuhara S., Mochizuki N., Nathans J., Stainier D.Y.R. Tip cell specificrequirement for an atypical Gpr124 and Reck-dependent Wnt/β-catenin pathway during
brain angiogenesis. eLife 2015 Jun 8;4:e06489. doi: 10.7554/eLife.06489.
３３. Mikelis C.M., Simaan M., Ando K., Fukuhara S., Sakurai A., Amornphimoltham P.,
Masedunskas A., Weigert R., Chavakis T., Adams R., Offermanns S., Mochizuki N.,
Zheng Y., Gutkind J.S. RhoA and ROCK mediate histamine-induced vascular leakage andanaphylactic shock. Nat. Commun. 2015 Apr 10;6:6725. doi: 10.1038/ncomms7725.
３４. Wakayama Y., Fukuhara S. (Corresponding author), Ando K., Matsuda M., Mochizuki N.
Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of
endothelial filopodia in zebrafish. Dev. Cell 2015 Jan 12;32(1):109-22. doi:
10.1016/j.devcel.2014.11.024.
３５. Kashiwada T., Fukuhara S. (Co-corresponding author), Terai K., Tanaka T., Wakayama Y.,
Ando K., Nakajima H., Fukui H., Yuge S., Saito Y., Gemma A., Mochizuki N. -Catenin-dependent transcription is central to Bmp-mediated formation of venous vessels.
Development 2015 Feb 1;142(3):497-509. doi: 10.1242/dev.115576.
３６. Fukui H., Terai K., Nakajima H., Chiba A., Fukuhara S., Mochizuki N. S1P-Yap1 signaling
regulates endoderm formation required for cardiac precursor cell migration in zebrafish.
Dev. Cell 31: 128-136 (2014).
３７. Fukuhara S. (Co-corresponding author), Zhang J., Yuge S., Ando K., Wakayama Y.,
Sakaue-Sawano A., Miyawaki A., Mochizuki N. Visualizing the cell-cycle progression ofendothelial cells in zebrafish. Dev. Biol. 393: 10-23 (2014).
３８. Odagiri H., Kadomatsu T., Endo M., Masuda T., Morioka M.S., Fukuhara S., Miyamoto T.,
Kobayashi E., Miyata K., Aoi J., Horiguchi H., Nishimura N., Terada K., Yakushiji T.,
Manabe I., Mochizuki N., Mizuta M., Oike Y. The secreted protein ANGPTL2 promotes
metastasis of osteosarcoma cells through integrin 51, p38 MAPK, and matrix
metalloproteinases. Sci. Signal. 7: ra7 (2014).
３９. Nakajima K., Shibata Y., Hishikawa Y., Suematsu T., Mori M., Fukuhara S., Koji T.,
Sawase T., Ikeda T. Coexpression of Ang1 and Tie2 in odontoblasts of mouse developing
and mature teeth―a new insight into dentinogenesis. Acta Histochem. Cytochem. 47: 1925 (2014).
４０. Ando K., Fukuhara S. (Co-corresponding author), Moriya T., Obara Y. Nakahata N.,
Mochizuki N. Rap1 potentiates endothelial cell junctions by spatially controlling myosin II
activity and actin organization. J. Cell Biol. 202: 901-916 (2013).
４１. Kwon H.B., Fukuhara S., Asakawa K., Ando K., Kashiwada T., Kawakami K., Hibi M.,
Kwon Y.G., Kim K.W., Alitalo K., Mochizuki N. The parallel growth of motoneuron
axons with the dorsal aorta depends on Vegfc/Vegfr3 signal in zebrafish. Development
140: 4081-4090 (2013).
４２. Mikelis C.M., Palmby T.R., Simaan M., Li W., Szabo R., Lyons R., Martin D., Yagi H.,
Fukuhara S., Chikumi H., Galisteo R., Mukouyama Y., Bugge T.H., Gutkind J.S. PDZRhoGEF and LARG are essential for embryo development, and provide a link between
thrombin and LPA receptors and Rho activation. J. Biol. Chem. 288: 12232-12243 (2013).
４３. Fukuhara S. (Co-corresponding author), Simmons S., Kawamura S., Inoue A., Orba Y.,
Tokudome T., Sunden Y., Arai Y., Moriwaki K., Ishida J., Uemura A, Kiyonari H., Abe
T., Fukamizu A., Hirashima M., Sawa H., Aoki J., Ishii M, Mochizuki N. The sphingosine1-phosphate transporter Spns2 expressed on endothelial cells regulates lymphocyte
trafficking in mice. J. Clin. Invest. 122: 1416-1426 (2012).
４４. Kusuhara S., Fukushima Y., Fukuhara S., Jakt L.M., Okada M., Shimizu Y., Hata M.,
Nishida K., Negi A., Hirashima M., Mochizuki N., Nishikawa S., Uemura A. Arhgef15
promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation andpotentiating RhoJ inactivation in endothelial cells. PLoS One 7: e45858 (2012).
４５. Takenouchi T., Iwamaru Y., Imamura M., Fukuhara S., Sugama S., Sato M., Mochizuki N.,
Hashimoto M., Yokoyama T., Mohri S., Kitani H. Cytochalasin D enhances theaccumulation of a protease-resistant form of prion protein in ScN2a Cells: Involvement of
PI3 kinase/Akt signaling pathway. Cell Biol. Int. 36: 1223-1231 (2012).
４６. Endo M., Nakano M., Kadomatsu T., Fukuhara S., Kuroda H., Mikami S., Hato T., Aoi J.,
Horiguchi H., Miyata K., Odagiri H., Masuda T., Harada M., Horio H., Hishima T., Nomori
H., Ito T., Yamamoto Y., Minami T., Okada S., Takahashi T., Mochizuki N., Iwase H., OikeY. Tumor cell-derived angiopoietin-like protein ANGPTL2 is a critical driver of metastasis.
Cancer Res. 72: 1784-1794 (2012).
４７. Makita N., Seki A., Sumitomo N., Chkourko H., Fukuhara S., Watanabe H., Shimizu W.,
Bezzina C.R., Hasdemir C., Mugishima H., Makiyama T., Baruteau A., Baron E., Horie M.,
Hagiwara N., Wilde A.A., Probst V., Le Marec H., Roden D.M., Mochizuki N., Schott J.J.,
Delmar M. A connexin 40 mutation associated with a malignant variant of progressive
familial heart block type-1. Circ. Arrhythm. Electrophysiol. 5: 163-172 (2012).
４８. Minami M., Koyama T., Wakayama Y., Fukuhara S. (Corresponding author), Mochizuki N.
EphrinA/EphA signal facilitates insulin-like growth factor-I-induced myogenic
differentiation through suppression of the Ras/extracellular signal-regulated kinase 1/2
cascade in myoblast cell lines. Mol. Biol. Cell 22: 3508-3519 (2011).
４９. Zhang J., Fukuhara S. (Co-corresponding author), Sako K., Takenouchi T., Kitani H.,
Kume T., Koh G.Y., Mochizuki N. Angiopoietin-1/Tie2 signal augments basal Notch signal
controlling vascular quiescence by inducing delta-like 4 expression through AKT-mediatedactivation of -catenin. J. Biol. Chem. 286: 8055-8066 (2011).
５０. Noda K., Zhang J., Fukuhara S. (Co-corresponding author), Kunimoto S., Yoshimura M.,
Mochizuki N. Vascular endothelial-cadherin stabilizes at cell-cell junctions by anchoring tocircumferential actin bundles through - and -catenins in cyclic AMP-Epac-Rap1 signalactivated endothelial cells. Mol. Biol. Cell 21: 584-596 (2010).
５１. Fujimoto C., Ozeki H., Uchijima Y., Suzukawa K., Mitani A., Fukuhara S., Nishiyama K.,
Kurihara Y., Kondo K., Aburatani H., Kaga K., Yamasoba T., Kurihara H. Establishmentof mice expressing EGFP in the placode-derived inner ear sensory cell lineage and FACSarray analysis focused on the regional specificity of the otocyst. J. Comp. Neurol. 518:
4702-4722 (2010).
５２. Tabata M., Kadomatsu T., Fukuhara S., Miyata K., Ito Y., Endo M., Urano T., Zhu H.J.,
Tsukano H., Tazume H., Kaikita K., Miyashita K., Iwawaki T., Shimabukuro M., Sakaguchi
K., Ito T., Nakagata N., Yamada T., Katagiri H., Kasuga M., Ando Y., Ogawa H., Mochizuki
N., Itoh H., Suda T., Oike Y. Angiopoietin-like protein 2 promotes chronic adipose tissue

inflammation and obesity-related systemic insulin resistance. Cell Metabolism 10: 178188 (2009).
５３. Sako K., Fukuhara S. (Corresponding author), Minami T., Hamakubo T., Song H., Kodama
T., Fukamizu A., Gutkind J.S., Koh G.Y., Mochizuki N. Angiopoietin-1 induces KrüppelLike Factor 2 expression through a phosphoinositide 3-kinase/AKT-dependent activation of
myocyte enhancer factor 2. J. Biol. Chem. 284: 5592-5601 (2009).
５４. Fukuhara S. (Co-corresponding author), Sako K., Minami T., Noda K., Kim H.Z., Kodama
T., Shibuya M., Takakura N., Koh G.Y., Mochizuki N. Differential function of Tie2 at cellcell contacts and cell-substratum contacts regulated by angiopoietin-1. Nat. Cell Biol. 10:513-526 (2008).
５５. Mukai H., Kikuchi M., Fukuhara S., Kiso Y., Munekata E.

Cryptidesignaling:amphiphilic peptide-induced exocytosis mechanisms in mast cells. Biochem. Biophys.
Res. Commun. 375: 22-26 (2008).
５６. Makita N., Behr E., Shimizu W., Horie M., Sunami A., Crotti L., Schulze-Bahr E.,
Fukuhara S., Mochizuki N., Makiyama T., Itoh H., Christiansen M., McKeown P.,
Miyamoto K., Kamakura S., Tsutsui H., Schwartz P.J., George Jr A. L., Roden D. M. The
E1784K mutation in SCN5A is associated with mixed clinical phenotype of type 3 long QT
syndrome. J. Clin. Invest. 118: 2219-2229 (2008).
５７. Yasuda N., Miura S., Akazawa H., Tanaka T., Qin Y., Kiya Y., Imaizumi S., Fujino M., Ito
K., Zou Y., Ge J., Fukuhara S., Kunimoto S., Mochizuki N., Fukuzaki K., Sato T., Nakaya
H., Saku K., Komuro I. Conformational switch of angiotensin II type 1 receptor underlying
mechanical stress-induced activation. EMBO Rep. 9: 179-186 (2008).
５８. Kamide K., Kokubo Y., Fukuhara S., Hanada H., Yang J., Kada A., Nagura J., Takiuchi S.,
Horio T., Kawano Y., Okayama A., Tomoike H., Miyata T. Protein Tyrosine Kinase 2β as a
Candidate Gene for Hypertension. Pharmacogenet. Genomics.17: 931-939 (2007).
５９. Yamaguchi Y., Nagase T., Tomita T., Nakamura K., Fukuhara S., Amano T., Yamamoto H.,
Ide Y., Suzuki M., Teramoto S., Asano T., Kangawa K., Nakagata N., Ouchi Y., Kurihara H.
-defensin overexpression induces progressive muscle degeneration in mice. Am. J.
Physiol. Cell Physiol. 292: C2141-C2149 (2007).
６０. Sakurai A., Fukuhara S., Yamagishi A., Sako K., Kamioka Y., Masuda M., Nakaoka Y.,
Mochizuki N. MAGI-1 is required for Rap1 activation upon cell-cell contact and forenhancement of vascular endothelial cadherin–mediated cell adhesion. Mol. Biol. Cell 17:
966-976 (2006).
６１. Somekawa S., Fukuhara S., Nakaoka Y., Fujita H., Saito Y., Mochizuki N. Enhanced
functional gap junction neoformation by PKA-dependent and Epac-dependent signals
downstream of cAMP in cardiac myocytes. Circ. Res. 97: 655-662 (2005).

６２. Ohkawara H., Ishibashi T., Sakamoto T., Sugimoto K., Nagata K., Yokoyama K., SakamotoN., Kamioka M., Matsuoka I., Fukuhara S., Sugimoto N., Takuwa Y., Maruyama Y.
Thrombin-induced rapid geranylgeranylation of RhoA as an essential process for RhoA
activation in endothelial cells. J. Biol. Chem. 280: 10182-10188 (2005).
６３. Fujita H., Fukuhara S., Sakurai A., Yamagishi A., Kamioka Y., Nakaoka Y., Masuda M.,
Mochizuki N. Local activation of Rap1 contributes to directional vascular endothelial cell
migration accompanied with extension of microtubules on which RapL, a Rap1-associating
molecule, localizes. J. Biol. Chem. 280: 5022-5031 (2005).
６４. Fukuhara S., Sakurai A., Sano H., Yamagishi A., Somekawa S., Takakura N., Saito Y.,
Kangawa K., Mochizuki N. Cyclic AMP potentiates VE-cadherin–mediated cell-cell
contact to enhance endothelial barrier function through an Epac-Rap1 signaling pathway.
Mol. Cell. Biol. 25: 136-146 (2005).
６５. Fukuhara S., Kurihara Y., Arima Y., Yamada N., Kurihara H. Temporal requirement ofsignaling cascade involving endothelin-1/endothelin receptor type A in branchial archdevelopment. Mech. Dev. 121: 1223-1233 (2004).
６６. Kamioka Y., Fukuhara S., Sawa H., Nagashima K., Masuda M., Matsuda M., Mochizuki N.
A novel dynamin-associating molecule, forming-binding protein 17, induces tubular
membrane invaginations and participates in endocytosis. J. Biol. Chem. 279: 40091-40099(2004).
６７. Murakami M., Kataoka K., Fukuhara S., Nakagawa O., Kurihara H. Akt-dependent
phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting
the DNA binding activity. Eur. J. Biochem. 271: 3330-3339 (2004).
６８. Endo A., Fukuhara S., Masuda M., Ohmori T., Mochizuki N. Selective inhibition ofvascular endothelial growth factor receptor-2 (VEGFR2) identifies a central role for
VEGFR2 in human aortic endothelial cell response to VEGF. J. Recept. Signal
Transduct. Res. 23: 239-54 (2003).
６９. Chikumi H., Fukuhara S., Gutkind J.S. Regulation of G protein-linked guanine nucleotide
exchange factors for Rho, PDZ-RhoGEF and LARG, by tyrosine phosphorylation:
Evidence of a role for FAK. J. Biol. Chem. 227: 12463-12467 (2002).
７０. Yamaguchi Y., Nagase T., Makita R., Fukuhara S., Tomita T., Tominaga T., Kurihara H.,
Ouchi Y. Identification of multiple novel epididymis-specific -defensin isoforms in thehumans and mice. J. Immunol. 169: 2516-2523 (2002).
７１. Yamaguchi Y., Fukuhara S., Nagase T., Tomita T., Hitomi S., Kimura S., Kurihara H.,
Ouchi Y. A novel mouse -defensin, mBD-6, predominantly expressed in skeletal muscle.
J. Biol. Chem. 276: 31510-31514 (2001).
７２. Fukuhara S., Chikumi H., Gutkind J.S. Leukemia-associated Rho guanine nucleotide

exchange factor (LARG) links heterotrimeric G proteins of the G12 family to Rho. FEBS Lett.
485: 183-188 (2000).
７３. Fukuhara S., Marinissen M.J., Chiariello M., Gutkind J.S. Signaling from G proteincoupled receptors to ERK5/BMK1 involves Gq and G12/13 families of heterotrimeric G
proteins: Evidence for the existence of a novel Ras and Rho-independent pathway. J. Biol.
Chem. 275: 21730-21736 (2000).
７４. Chiang Y.J., Kole H.K., Brown K., Naramura M., Fukuhara S., Hu R.-J., Jang I.K., Gutkind
J.S., Shevach E., Gu H. Cbl-b regulates the CD28 dependence of T-cell activation. Nature
403: 216-220 (2000).
７５. Murga C., Fukuhara S., Gutkind J.S. A novel role for phosphatidylinositol 3-kinase  insignaling from G protein-coupled receptors to Akt. J. Biol. Chem. 275: 12069-12073(2000).
７６. Pirone D.M., Fukuhara S., Gutkind J.S., Burbelo P.D. SPECs, small binding proteins for
Cdc42. J. Biol. Chem. 275: 22650-22656 (2000).
７７. Fukuhara S., Murga C., Zohar M., Igishi T., Gutkind J.S. A novel PDZ-domain containing
guanine nucleotide exchange factor links heterotrimeric G proteins to Rho. J. Biol. Chem.
274: 5868-5879 (1999).
７８. Igishi T., Fukuhara S., Patel V., Katz B.-Z., Yamada K.M., Gutkind J.S. Divergent signaling
pathways link focal adhesion kinase to mitogen-activated protein kinase cascades:
Evidence for a role of paxillin in c-Jun NH2-terminal kinase activation. J. Biol. Chem.
274: 30738-30746 (1999).
７９. Fukuhara S., Shimizu M., Matsushima H., Mukai H., Munekata E. Signaling pathways via
NK1 receptors and their desensitization in an AR42J cell line. Peptides 19: 1349-1357(1998).
８０. Fukuhara S., Mukai H., Munekata E. Activin A and all-trans-retinoic acid cooperatively
2+enhanced the functional activity of L-type Ca channels in the neuroblastoma C1300 cellline. Biochem. Biophys. Res. Commun. 241: 363-368 (1997).
８１. Fukuhara S., Mukai H., Kako K., Nakayama K., Munekata E. Further identification ofneurokinin receptor types and mechanisms of calcium signaling evoked by neurokinins in
the murine neuroblastoma C1300 cell line. J. Neurochem. 67: 1282-1292 (1996).
８２. Fukuhara S., Mukai H., Munekata E. Pharmacological evidence for neurokinin receptors inmurine neuroblastoma C1300 cells. Peptides 16: 211-214 (1995).