The 90th Anniversary Meeting

The Society for Biotechnology, JapanInternational Symposium on
BIOTECHNOLOGY FOR

Program
October 24-26, 2012
Kobe, Japan

Organized by
The Society for Biotechnology, Japan (SBJ)
Japan Science and Technology Agency (JST)Supported by
Kobe Convention & Visitors Association
Suntory Institute for Bioorganic Research

Nakauchi Tsutomu Convention Shinko Zaidan Foundation

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Advances in PhytoGenomics
Organizers: Toshiya MURANAKA (Osaka University)
Kiichi FUKUI (Osaka University)

Wed 24, OCT

10/24 (Wednesday)

Chairperson: Kiichi FUKUI (Osaka University)
9:00–
9:25

2Fa01

Functional chromosome dynamics with a link to synthetic
green biotechnology○Ingo SCHUBERT

9:25–
9:45

2Fa02

Manipulation of plant traits using novel gene silencing system,
CRES-T (Chimeric REpressor gene Silencing Technology)○Nobutaka MITSUDA1, Masaru OHME-TAKAGI1,2(1Bioproduction Research Institute, National Institute of Advanced
Industrial Science and Technology (AIST), Japan, 2Institute forEnvironmental Science and Technology (IEST), Saitama University,Japan)

9:45–
10:05

2Fa03

A top-down approach to function the Synechocystis PCC6803
genome in a novel cloning host Bacillus subtilis 168○Mitsuhiro ITAYA(Institute for Advanced Biosciences, Keio University, Japan)

Chairperson: Toshiya MURANAKA (Osaka University)
10:05–
10:30

2Fa04

Decoding the chemical diversity and evolution written in thegenomes of the Asteraceae○Dae-Kyun RO1, Don Trinh NGUYEN1, Nobuhiro IKEZAWA 2,Vince QU1(1Department of Biological Sciences, University of Calgary, Alberta,Canada, 2Department of Biotechnology, Osaka University, Japan)

10:30–
10:55

2Fa05

Cassava molecular breeding and functional genomics–Challenges and opportunities–○Jarunya NARANGAJAVANA(Center for Cassava Molecular Biotechnology, Department ofBiotechnology, Faculty of Science, Mahidol University, Bangkok,Thailand)

10:55–
11:15

2Fa06

KOMICS: metabolomics databases and tools for biotechnology○Daisuke SHIBATA, Takeshi ARA, Hideyuki SUZUKI,
Nozomu SAKURAI(Kazusa DNA Research Institute, Chiba, Japan)

Program(Department of Cytogenetics and Genome Analysis, Leibniz
Institute of Plant Genetics and Crop Plant Research (IPK), Germany)

Wed 24, OCT

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Chairperson: Kazuhito FUJIYAMA (Osaka University)
11:15–
11:40

2Fa07

Dammarene-type ginsenoside metabolic engineering–Characterization of genes involved in dammarenediol saponinbiosynthesis–
Jung-Yeon HAN, ○Yong-Eui CHOI
(Department of Forest Resources, College of Forest andEnvironmental Science, Kangwon National University, South Korea)

11:40–
12:00

2Fa08

Production of plant triterpenoids in engineered yeast○Toshiya MURANAKA, Hikaru SEKI

Program(Department of Biotechnology, Graduate School of Engineering,Osaka University, Japan)

Bio-Based Production
Organizers: Hiroshi SHIMIZU (Osaka Univeristy)
Eiichiro FUKUSAKI (Osaka University)
Chairperson: Hiroshi SHIMIZU (Osaka Univeristy)
13:50–
14:20

2Ap01 Living microbes as selective and efficient redox biocatalysts○Andreas SCHMID, Katja BÜHLER, Mattijs JULSING,
Bruno BÜHLER(Laboratory of Chemical Biotechnology, TU Dortmund University,Germany)

14:20–
14:50

2Ap02 Toward biological replacement of petroleum○James C. LIAO
(Department of Chemical and Biomolecular Engineering,
University of California, Los Angeles, USA)

14:50–
15:20

2Ap03 Bacillus subtilis cell factory for production of scyllo-inositol
promising for Alzheimer’s disease○Ken-ichi YOSHIDA1, Chumsakul ONUMA 2, Shu ISHIKAWA 2,
Naotake OGASAWARA 2(1 Department of Agrobioscience, Graduate School of AgriculturalScience, Kobe University, Japan, 2 Graduate School of BiologicalSciences, Nara Institute of Science and Technology (NAIST), Japan)

15:20–
15:50

2Ap04 Engineering of signal transduction for enhanced production ofbiopharmaceuticals and bioenergy○Jian-Jiang ZHONG1, Yang-Chun YONG2(1 School of Life Sciences and Biotechnology, Shanghai Jiao Tong
University, China, 2 School of the Environment, Jiangsu University,China)

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2Ap05 Synthetic biology for the comprehension of biomolecularnetworks○Masahiro OKAMOTO1,2(1 Department of Bioinformatics, Graduate School of Systems Life
Sciences, Kyushu University, Japan, 2 Synthetic Systems Biology
Research Center (SSBRC), Kyushu University, Japan)

16:20–
16:30

BREAK

2Ap06 Strategies and technologies towards tailor made fuels frombiomass○Jochen BUECHS1, Tobias KLEMENT1, Martin KUNZE1,Gernot JAEGER1, Sandra WEWETZER1,Frederike CARSTENSEN2, Matthias WESSLING2,Philipp GRANDE3, Pablo Domínguez DE MARIA3, Antje SPIESS4(1 AVT - Biochemical Engineering, RWTH Aachen University,Germany, 2 AVT - Chemical Process Engineering, RWTH Aachen
University, Germany, 3 Institute of Technical and Macromolecular
Chemistry, RWTH Aachen University, Germany, 4 AVT - Enzyme
Process Technology, RWTH Aachen University, Germany)

17:00–
17:30

2Ap07 Actinobacteria tyrosinase and its applications to biomaterials○Byung-Gee KIM1,2,3, Nahum LEE1,3, SangHyuk LEE2,3,ChangHyun SONG1,3(1 School of Chemical and Biological Engineering, Seoul National
University, Korea, 2 Institute of Molecular Biology and Genetics,
Seoul National University, Korea, 3 Institute of Bioengineering,Seoul National University, Korea)

17:30–
18:00

2Ap08 Cyberinfrastructure for metabolomics and synthetic biology○Masanori ARITA1,2(1Department of Biophysics and Biochemistry, Graduate School ofScience, The University of Tokyo, Japan, 2 RIKEN Plant Science
Center, Japan)

18:00–
18:30

2Ap09 Bioprocess engineering on biofuels and bio-based chemicals
production from microalgae○Jo-Shu CHANG1,2,3, Chun-Yen CHEN2(1Department of Chemical Engineering, National Cheng Kung
University, Taiwan, 2Center for Bioscience and Biotechnology,National Cheng Kung University, Taiwan, 3Research Center for
Energy Technology and Strategy, National Cheng Kung University,Taiwan)

ProgramChairperson: Eiichiro FUKUSAKI (Osaka University)
16:30–
17:00

Wed 24, OCT

15:50–
16:20

Wed 24, OCT

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18:30–
19:00

2Ap10 Systems metabolic engineering –Rational design of microbial
cell factories○Hiroshi SHIMIZU1, Chikara FURUSAWA1,2, Takashi
HIRASAWA1, Naoaki ONO3, Katsunori YOSHIKAWA1,Yoshihiro TOYA1

Program(1 Department of Bioinformatic Engineering, Osaka University,Japan, 2 Quantitative Biology Center (QBiC), RIKEN, Japan,
3Graduate School of Information Science, Nara Institute ofScience and Technology (NAIST), Japan)

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Biomolecular Engineering [co-organized by Young Asian
Biochemical Engineers’ Community (YABEC)]
Organizers: Takeshi OMASA (University of Tokushima)
Noriho KAMIYA (Kyushu University)

Thu 25, OCT

10/25 (Thursday)

Chairperson: Takeshi OMASA (University of Tokushima)
9:00–
9:30

3Fa01

Reaction evaluation and new process design in composting ofbiological wastes~Young Asian Biotechnologist Prize~○Jingchun TANG1, Arata KATAYAMA 2

9:30–
9:40

BREAK

Chairperson: Noriho KAMIYA (Kyushu University)
9:40–
10:05

3Fa02

Organic solvent stable enzymes○Hiroyasu OGINO(Department of Chemical Engineering, Osaka Prefecture University,Japan)

10:05–
10:30

3Fa03

Engineering of aminoacyl-tRNA synthetases for residuespecific incorporation of amino acid analogues into proteins○Tae Hyeon YOO(Department of Molecular Science and Technology, Department ofApplied Chemistry and Biological Engineering, Ajou University,Korea)

10:30–
10:55

3Fa04

Production of the bio-butanol based on the continuous in situ
fermentation and adsorption (ISFA) technology○Hanjie YING1,2, Yong CHEN2,3, Jinglan WU2,3(1State Key Laboratory of Materials-Oriented Chemical Engineering,
China, 2 College of Biotechnology and Pharmaceutical Engineering,
Nanjing University of Technology, China, 3 National Engineering
Technique Research Center for Biotechnology, China)

Program(1College of Environmental Science and Engineering, Nankai
University, China, 2 EcoTopia Science Institute, Nagoya University,Japan)

Thu 25, OCT

84

Chairperson: Takeshi OMASA (University of Tokushima)
10:55–
11:20

3Fa05

Genomic engineering as a new technology platform formetabolic engineering of microorganisms○Yun-Peng CHAO1, Chung-Jen CHIANG2, Po Ting CHEN3(1Department of Chemical Engineering, Feng Chia University,Taiwan, 2Department of Medical Laboratory Science andBiotechnology, China Medical University, Taiwan, 3Department ofBiotechnology, Southern Taiwan University, Taiwan)

Program

11:20–
11:45

3Fa06

Enzyme engineering with the aid of ionic liquids○Noriho KAMIYA1,2(1Center for Future Chemistry, Kyushu Univeristy, Japan,
2
Department of Applied Chemistry, Graduate School ofEngineering, Kyushu University, Japan)

SBJ/JST Joint Symposium I –General Session–
Organizers: Tadashi MATSUNAGA (Tokyo University of Agriculture and Technology)
Hisao OHTAKE (Osaka University)
Shigeru ISHIMASA (JST)
Chairperson: Kohsuke HONDA (Osaka University)
14:00–
14:05

Welcome adress○Hisao OHTAKE(Department of Biotechnology, Graduate School of Engineering,Osaka University, Japan)

14:05–
14:15

3Ap01 Introduction of the JST CREST/PRESTO program researcharea “Creation of basic technology for improved bioenergy
production through functional analysis and regulation of algae
and other aquatic microorganisms”○Tadashi MATSUNAGA(Tokyo University of Agriculture and Technology, Japan)

Chairperson: Masafumi YOHDA (Tokyo University of Agriculture and Technology)
14:15–
14:50

3Ap02 Can biotechnology address the global energy crisis?
○Jonathan D. TRENT1,2(1 Bioengieering Branch, NASA Ames Research Center, USA,
2
Biomolecular Engineering, University of California Santa Cruz, USA)85

14:50–
15:25

3Ap03 Food and fuel from microalgae: Integration of photosynthesis
and photovoltaics allows to achieve a positive energy balanceof algae biomass production○Mario R. TREDICI

Thu 25, OCT

Chairperson: Akira SATO (Yamaha Motor, Co.)

(University of Florence, Italy)
15:25–
15:35

BREAK

15:35–
16:10

3Ap04 Biorefinery of microalgae○René H. WIJFFELS(Wageningen University, Bioprocess Engineering, the Netherlands)

Chairperson: Kohsuke HONDA (Osaka University)
16:10–
16:20

Closing remarks○Shigeru ISHIMASA(Japan Science and Technology Angency (JST), Japan)

Japan-Korea Biomass Session
Organizers: Atsuhiko SHINMYO (Nara Institute of Science and Technology)
Shigeaki FUJIKAWA (NPO Kinki Bio-Industry Development Organization)
Chairperson: Shigeaki FUJIKAWA (NPO Kinki Bio-Industry Development Organization)
16:40–
16:45

Opening remarks○Atsuhiko SHINMYO(Nara Institute of Science and Technology, Japan)

16:45–
17:05

3Ap05 Improvement of ethanol yield from xylose by breeding of industrial
yeast○Haruyo HATANAKA, Tomohide MAEDA, Yuri YASUE, KazueNISHIYAMA(Suntory Business Expert Limited, Institute for Microbial Science,Japan)

ProgramChairperson: Tsuyoshi TANAKA (Tokyo University of Agriculture and Technology)

Thu 25, OCT

86

17:05–
17:25

3Ap06 Applications of microbial factory technology to production ofbioethanol from cellulosic biomass
Jung-Hyun JO1, Sang-Min JUNG1, Yong-Cheol PARK 2,Yong-Su JIN3, ○Jin-Ho SEO1

Program(1Department of Agricultural Biotechnology, Seoul National
University, Korea, 2 Department of Advanced Fermentation Fusion
Science and Technology, Kookmin University, Korea, 3 Departmentof Food Science and Human Nutrition, University of Illinois atUrbana-Champaign, USA)
17:25–
17:45

3Ap07 Potential in bioethanol production from waste paper sludge inpulp-based biorefinery○Enoch Y. PARK1,2, Joni PRASETYO1, Kazuya NARUSE2,Tatsuya KATO2(1 Laboratory of Biotechnology, Graduate School of Science andTechnology, Shizuoka University, Japan, 2 Laboratory ofBiotechnology, Faculty of Agriculture, Shizuoka University, Japan)

17:45–
17:55

BREAK

Chairperson: Moon-Hee SUNG (Kookmin University)
17:55–
18:15

3Ap08 Application of Metabolomics to high resolution phenotypeanalysis○Eiichiro FUKUSAKI(Department of Biotechnology, Graduate School of Engineering,Osaka University, Japan)

18:15–
18:35

3Ap09 Biological production of 3-hydroxypropionic acid from glycerol○Sunghoon PARK
(School of Chemical and Biomolecular Engineering, Pusan NationalUniversity, Korea)

18:35–
18:55

3Ap10

Biodiesel production by enzymatic process and the utilizationof crude glycerol
Hah Young YOO, Laxmi Prasad THAPA, Ja Hyun LEE,
Sang Jun LEE, ○Seung Wook KIM(Department of Chemical and Biological Engineering, Korea
University, Korea)

18:55–
19:00

Closing remarks○Sunghoon PARK
(Pusan National University, Korea)

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SBJ/JST Joint Symposium II –Biorefinery–
Organizers: Haruko TAKEYAMA (Waseda University)
Yoshihiro SHIRAIWA (Tsukuba University)

Fri 26, OCT

10/26 (Friday)

Chairperson: Haruko TAKEYAMA (Waseda University)
9:00–
9:25

4Aa01

Understanding and altering the unique metabolism ofhyperthermophilic archaea○Haruyuki ATOMI1,2, Kunio MIKI2,3, Tadayuki IMANAKA 2,4

9:25–
9:50

4Aa02 Blue ocean for blue biotechnology – Marine bioenergy
production○Choul-Gyun LEE1,2(1Department of Biotechnology, Inha University, Korea,
2
Korea National Consortium on Marine Bioenergy Research)

9:50–
10:15

4Aa03 Establishment of innovative technology to create newmicroalgal strains increasing biofuel production bypolyploidization and heavy-ion beam irradiation○Shigeyuki KAWANO1,2, Koichi WATANABE1,2, Shuhei OTA1,2(1Department of Integrated Biosciences, Graduate School of Frontier
Sciences, The University of Tokyo, Japan, 2CREST, Japan Scienceand Technology Agency (JST), Japan)

Chairperson: Yoshihiro SHIRAIWA (Tsukuba University)
10:15–
10:40

4Aa04 New dimensions in microalgae biomass production forbioenergy○Otto PULZ
(IGV, Nuthetal, Germany)

10:40–
10:45

BREAK

Program(1Department of Synthetic Chemistry and Biological Chemistry,Graduate School of Engineering, Kyoto University, Japan,2CREST, Japan Science and Technology Agency (JST),
3
Department of Chemistry, Graduate School of Science, Kyoto
University, Japan, 4Department of Biotechnology, College of Life
Sciences, Ritsumeikan University, Japan)

Fri 26, OCT

88

Chairperson: Yoshihiro SHIRAIWA (Tsukuba University)
10:45–
11:10

4Aa05 Development of sustainable biorefinery based on microalgae○Akihiko KONDO1,2(1Department of Chemical Science and Engineering, Graduate
School of Engineering, Kobe University, Japan, 2Core Research
for Evolutional Science and Technology (CREST), Japan Scienceand Technology Agency (JST), Japan)

Program

11:10–
11:35

4Aa06 Chlamydomonas reinhardtii - A powerful host for the productionof biofuels and high value products○Olaf KRUSE(Bielefeld University, Department of Biology/ Center for Biotechnology,Algae Biotechnology & Bioenergy Group, Germany)

Chairperson: Haruko TAKEYAMA (Waseda University)
11:35–
12:00

4Aa07 Alkenone biosynthesis by marine Haptophytes as biorefinery
for renewable energy production○Yoshihiro SHIRAIWA1,2(1Faculty of Life & Environmental Sciences, University of Tsukuba,
Japan, 2 CREST, Japan Science and Technology Agency (JST),Japan)

SBJ/JST Joint Symposium III –Biofuel Production by Microalgae–Organizers: Masayuki OHMORI (Chuo University)
Shigeru OKADA (The University of Tokyo)
Chairperson: Masayuki OHMORI (Chuo University)
14:00–
14:20

4Ap01 Characterization of hydrocarbon biosynthesis and secretionmechanisms by the green microalga, Botryococcus braunii tocontrol biofuel production○Shigeru OKADA1,5, Ikuro ABE2,5, Tetsuko NOGUCHI3,5,Takeshi OHAMA4,5(1Department of Aquatic Biosciences, Graduate School of Agriculturaland Life Sciences, The University of Tokyo, Japan, 2 Graduate Schoolof Pharmaceutical Sciences, The University of Tokyo, Japan,
3
Department of Biological Sciences, Faculty of Science, Nara
Women’s University, Japan, 4 Department of Environmental Systems
Engineering, Kochi University of Technology, Japan, 5 CREST, Japan
Science and Technology Agency (JST), Japan)

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4Ap02 Metabolic engineering of cyanobacteria for the light-powered
production of hydrogen from water○Matthias RÖGNER
(Plant Biochemistry, Faculty of Biology & Biotechnology, RuhrUniversity Bochum, Germany)

14:55–
15:15

4Ap03 Selection strategies for the promising microalgal strains foralgae-fuel production○Hideaki MIYASHITA1, Norihide KURANO2

BREAK

Chairperson: Shigeru OKADA (The University of Tokyo)
15:25–
16:00

4Ap04 Elucidation of novel triterpene pathways in Botryococcus andengineering plants for high-value oil production
Tom D. NIEHAUS1, Scott KINISON1, Shigeru OKADA 2,Shuiqin WU1, Timothy P. DEVARENNE3, David S. WATT4,Vitaliy SVIRIPA4, ○Joe CHAPPELL1(1Plant Biology Program, University of Kentucky, USA, 2Department
of Aquatic Biosciences, University of Tokyo, Japan, 3Department ofBiochemistry and Biophysics, Texas A&M University, USA,
4
Department of Cellular and Molecular Biochemistry, University ofKentucky, USA)

16:00–
16:20

4Ap05 The CyanofactoryTM~ a novel cyanobacterial bioprocess basedon the synthetic biology concept ~○Koji SODE1,2(1Tokyo University of Agriculture & Technology, Japan, 2 Japan
Science and Technology Agency, CREST, Japan)

Program(1Department of Interdisciplinary Environment, Graduate School of
Human and Environmental Studies, Kyoto University, Japan,
2
Research laboratories, DENSO CORPORATION, Japan)
15:15–
15:25

Fri 26, OCT

14:20–
14:55

Fri 26, OCT

90

SBJ/JST Joint Symposium IV –System & Synthetic Biotechnology–
Organizers: Daisuke UMENO (Chiba University)
Tomohisa HASUNUMA (Kobe University)
Kohsuke HONDA (Osaka University)
Chairperson: Daisuke UMENO (Chiba University)

Program

16:40–
17:00

4Ap06 Bio-refinery from microalgae through fermentation andmetabolic engineering○Tomohisa HASUNUMA1,2(1Organization of Advanced Science and Technology, Kobe
University, Japan, 2PRESTO, Japan Science and Technology
Agency (JST), Japan)

17:00–
17:35

4Ap07 Synthetic biology approaches to produce C3-C5 alcohols frommicroorganisms○Shota ATSUMI(Department of Chemistry, University of California Davis, USA)

17:35–
17:55

4Ap08 Synthetic metabolic engineering –A novel, simple technology
for designing a chimeric metabolic pathway–○Kohsuke HONDA1,2, Xiaoting YE1, Kenji OKANO1,Hisao OHTAKE1(1 Department of Biotechnology, Graduate School of Engineering,Osaka University, Japan, 2 PRESTO, Japan Science and Technology
Agency (JST), Japan)

17:55–
18:05

BREAK

Chairperson: Tomohisa HASUNUMA (Kobe University)
18:05–
18:40

4Ap09 Production of BiolsopreneTM monomer○Derek H. WELLS(Du Pont Industrial Biosciences, USA)

18:40–
19:00

4Ap10 Evolutionary pathway engineering: toward the fast-track
construction of long-step, but high-fidelity pathway for nonnatural compounds○Daisuke UMENO1,2, Maiko FURUBAYASHI1, Kyoichi SAITO1(1Department of Applied Chemistry and Biotechnology, Graduate
School of Engineering, Chiba University, Japan, 2PRESTO, JapanScience and Technology Agency (JST), Japan)