Support For Metabolic Syndrome Research
OYC Research Reagents

Mouse/Rat Adiponectin
ELISA-OY
Adiponectin, also referred to as Acrp30, AdipoQ and GBP28 is aprotein which is highly expressed in adipose cells. Human adiponectin
was identified by Dr. Matsuzawa, Osaka University, whereas mouseadiponectin was identified by Lodish, MIT. It forms trimer, hexamer, andhigh molecular weight multimers in serum. Adiponectin plays a role inphysiological processes such as inflammation, anti diabetes,
arteriosclerosis, energy homeostasis, and obesity. Plasma levels ofadiponectin are reduced in obese patients, so it is thought as one ofnew biomarkers.
This kit is based on sandwich ELISA method using anti-mouseadiponectin rabbit polyclonal antibody, and content of adiponectin inmouse and rat sera or cell culture medium is available specifically andaccurately.
Item
Mouse/Rat Adiponectin ELISA-OY

Catalog number

Packing size47579900

96 tests＊For research purposes only. Not for therapeutic or diagnostic use.

Specifications

Measuring forms

Trimer, Hexamer, Multimer

Sample form

Serum, Plasma,
Culture medium

Pretreatment

NoneTotal reaction time

4 hours

Immobilized antibody

Rabbit polyclonal IgG

Detection antibody

Biotin-labeled rabbit
polyclonal IgG

StandardRecombinantmouse adiponectin

Calibration range

0.375-24 ng/mL

High reproducibility
Adiponectin (µg/mL)

www.oyc.co.jp

1. High reproducibility
2. Wide calibration range: 0.375 - 24 ng/mL.
3. Applicable to culture medium containing bovine serum

20151050
Mouse Serum Mouse SerumAB
Our Kit

6,161-fold (mouse)

Other kit

Dilution rate
808-fold (rat)

Coefficient variation

Sample dilution time

TwiceSampleMouseSerum A

Mouse
Serum B

Substrate solution

TMB 2 liquid type（1:1 mixture）

Our kit

2.2%

1.4%

Measurement
wavelength

450 nm / 570 nm

Other kit

5.3%

3.9%

※Rat adiponectin concentration is calculated as the equivalent of mouse adiponectin concentration.

Support For Metabolic Syndrome Research
OYC Research Reagents
Kit Components
No.

Components

Quantity

1

Washing buffer 10X

100 mL×1

2

Sample diluent

100 mL×13

Antibody-immobilized plate4

24 ng/mL mouse adiponectin standard

1.5 mL×1

5

Biotin-labeled antibody solution

12 mL×1

6

Enzyme-labeled streptavidin reaction solution

12 mL×17

Substrate solution A

6 mL×18

Substrate solution B

6 mL×19

Reaction stop solution

12 mL×18 well×12 modules

Related Products
Item

Catalog number

Packing size

Human Adiponectin

47620000

100 µgMouse Adiponectin

47024000

100 µg

8A147022000

500 µg

8B347021000

500 µg-47023000

500 µg

1F647046000

500 µg-47047000

100 µgAnti-Human
Adiponectin

Anti-Mouse
Adiponectin

www.oyc.co.jp

References
1．Maeda, K. et al. (1996): Biochem, Biophys. Res. Commun. 221, 286.
2．Schereer, PE. et al. (1995): J. Biol. Chem. 270, 26746.
3．Waki, H., (2003): J. Biol.Chem. 278, 40352.
4. Ohashi, K. et al. (2010): J. Biol. Chem. 285, 6153.

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(Mumbai, INDIA)
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Phone:+91-22-27717107
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202102