Support For Cell Culture
OYC Research Reagents

Recombinant Human Lamininsand Antibodies
Recombinant Human Laminin-3A32 (rLm3A32：rLm5A)
Recombinant Human Laminin-3B32 (rLm3B32：rLm5B)
Recombinant Human Laminin-3B11 (rLm3B11：rLm6B)
Anti-Human Laminin α3 Monoclonal Antibody
Anti-Human Laminin β3 Monoclonal Antibody
Laminins are extracellular matrix (ECM) proteins localized in thebasement membrane (BM) and composed of α, β and γ chains. At least
15 kinds of laminins have been identified so far.
Laminin-3A32 (Laminin-5A) has various unique biological activities and ismainly localized in epithelial BMs of skin and lung. Laminin-3B32 (Laminin5B) has stronger biological activities than Laminin-3A32 (Laminin-5A).
Laminin-3B11 (Laminin-6B) is widely localized in vascular BMs of normaltissues and applicable to the culture of vascular endothelial cell.
Cell adhesion activity of rLm3A32, rLm3B32 and rLm3B11
rLm3A32

rLm3B32

1 µg/mLGelatin

1 µg/mL

1 µg/mL

1 µg/mL

0.25µg/mLFig.1
Morphology of BRL (buffalo rat liver) cell after theculture for a hour on the surface treated with rLms
under serum-free condition. Adherent cells werestained with crystal violet.

Normal human umbilical vein endothelial cell (HUVEC) growth on rLm3B110.25

Fig.2
HUVECs were cultured
on the surface treatedwith various kinds ofECM proteins and thecell growth weremeasured by WST-1
reagent.

0.2

OD450-OD595

www.oyc.co.jp

0.25
µg/mL

rLm3B110.15
0.1
0.05
020.25rLm3B1142

EHS-Laminin

20.25

Fibronectin

1000300

GelatinMatrigel

PBS(-)µg/mL

Immunohistochemistry
Immunohistochemistry analysis of human tissues by laminin antibodies *.

Laminin α3 antibody
Fig.3

BM of skin tissue

BM of mammary gland

Laminin β3 antibody
BM of skin tissue

Skin cancer* The data of immunohistochemistry is provided by prof. Miyazaki of Yokohama City University.

Support For Cell Culture
OYC research reagentsSpecifications
Recombinant proteins
Host cell
OriginConcentration： HEK293： Human： 20 µg/mL(approx.)

Antibodies
Item

Application

Subclass

Crossreactivity

Anti-Human Laminin α3
Monoclonal Antibody

WBIHC

Mouse IgG1κ

HumanAnti-Human Laminin β3
Monoclonal Antibody

WBIHC

Mouse IgG1λHumanConcentration

0.8-1.2 µg/mL

ItemCatalog number

Packing size

47200000

2 µg

47201000

2 µg × 5

Recombinant Human Laminin-3B32 (rLm5B)

47032000

10 µg

Recombinant Human Laminin-3B11 (rLm6B)

47033000

10 µg

Anti-Human Lm α3 Monoclonal Antibody

47252000

100 µgAnti-Human Lm β3 Monoclonal Antibody

47253000

100 µgRecombinant human laminin-3A32 (rLm5A)＊ For bulk inquiry, please contact us below.
＊ For research purposes only. Not for therapeutic or diagnostic use.

www.oyc.co.jp

References
・ J Biochem. 2002 Oct;132 (4):607-12.
・ J Biol Chem. 2004 Jun 4;279 (23):24774-84.
・ J Biochem. 2005 Nov;138 (5):539-52.
・ Stem Cells. 2006 Nov;24 (11):2346-54.
・ J Mol Histol. 2008 Aug;39 (4):435-46.

Please contact the one that best suits your need.
ORIENTAL YEAST CO., LTD.
Corporate Headquarters (Tokyo, Japan)
URL:
https://www.oyc.co.jp/bio/E-mail:fbi@nisshin.com
Phone:+81-3-3968-1192
Fax:+81-3-3968-4863OYC EU B.V.

OYC Americas, Inc.
(Vista, USA)URL:
https://www.oycus.com/
Phone:+1-760-659-5943
Fax:+1-760-201-8950

ORIENTAL YEAST INDIA Pvt. Ltd.
(Mumbai, INDIA)
URL:
https://oycindia.com/
E-mail:info@oycindia.com
Phone:+91-22-27717107
Fax:+91-22-27717107(Rotterdam, The Netherlands)
URL:
https://www.oyceu.com/
E-mail:info@oyceu.com
Phone:+31-10-4145777
Fax:+31-10-2134919

202112