Mosaic 3
Real Time Parallel Illumination ofMultiple Regions
Key Features

Key Applications

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Wavelength range 360 to 800 nm

3

Optogenetics

3

Minimum exposure time 50 – 200 µs

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Optophysiology

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Maximum frame rate 5000 fps

3

Photoconversion

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Comprehensive DMD triggering modes

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Photoactivation

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Long lifetime and low maintenance3

Uncaging

3

Wide range of light sources

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Photobleaching

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Introduction | Features | Specifications & Ordering

Introducing Mosaic 3

Flexible Real-time Illumination Control

Andor’s Mosaic 3 is a patented instrument for targeted illumination for microscopy built
around MEMS Digital Mirror Devices (DMD). High speed frame switching up to 5,000 fps
makes the Mosaic 3 suitable for many dynamic applications including bleaching, uncaging,photo-switching, optogenetics and constrained illumination. Mosaic 3 exploits DMD in a
proprietary programmable platform, integrated with scientific light sources including lasers,LEDs and arc lamps, and operates from 360 to 800 nm.

The on-board memory allows for sequences of patterns to be defined and executed with real-time accuracy under software
control or following external trigger signals for ultimate experimental flexibility. A unique feature of the Mosaic family is thegreyscale functionality for region-specific illumination intensity control in addition the binary operation of the DMD mirrors.
333
33Performs simultaneous imaging and photo-stimulation
Delivers flexible, multi-ROI-specific control of illumination intensity
Augments wide range of techniques: optogenetics, bleaching/activation and uncaging
Ideal for high speed physiological studies
Integrate with electro/optophysiology workstations

DMD Technology
The core of Mosaic 3 is the Digital Mirror Device (DMD), a high speed and highly efficient semiconductor-based “light switch”
array of hundreds of thousands of hinge-mounted, addressable, tiltable, microscopic mirrors.
When a DMD chip is co-ordinated with a digital video or graphic signal, a light source, and beam delivery optics, its mirrors
reflect a digital image of the illumination mask onto the sample. This is represented in the illustrations below:

On

Applications GuideP
P

Optogenetics/Optophysiology

P

Uncaging

Cell signalling, ion imaging, neurotransmission

P

PhotobleachingProtein dynamics, protein turnover, cell compartmentalisation

Cell signalling, Neuronal signalling, Electrophysiology, opsin controlled secondary messengers

Photoconversion/photoactivation Cell dynamics, intracellular organelle tracking, cytoskeleton studies

OffEach tiltable mirror of the 800 x 600
DMD array in the Mosaic 3 can beswitched “on” or “off” to create therequired illumination mask for theimage.

Simultaneous multi-region photoactivationof GFP labelled actin in fibroblast.

Mosaic 3 is available in two models with two different fields of illumination and integrates withboth upright and inverted microscopes:
1) Large field models
Provide illumination to fill most of the field of view of the microscopeand match sCMOS camera FOVs. These models are commonly used for
applications such as optogenetics and photoactivation where lower power
densities are delivered by LEDs.

Release of Caged Compounds. Mosaic 3 can be used with
UV sources including arc lamps, LED and lasers to uncage
biomolecules and render them active (Eder and Bading, BMC
Neurosci. 2007; 8: 57).

Photoactivation of pa:GFP-histone in U2OS nuclei lights uphistones green in a linear region. This technique can be used tostudy histone mobility within the nucleus, in response to agonists
and relative to other nucleoli or other compartments.

Learn more about how Mosaic 3 can be used to expand our understanding of cellular processes in live cells
and model organisims:
3333

Illumination Solutions for Photoactivatable Fluorescent Proteins
Spatiotemporal Control of Cell Signaling using a Light-Switchable Protein Pair
Illumination solutions for Uncaging Experiments
Uncovering the Membrane Mechanism of Cytokinesis

2) Small field models
Matches smaller sensor formats such as Andor’s iXon 897 EMCCD and suited
to applications including bleaching and uncaging where higher power densities delivered by lasers are required.

23Introduction | Features | Specifications & Ordering

How to Integrate Mosaic 3 into your Microscope

Product Drawings: Mosaic Infinity Models (upright Microscopes)

Mosaic 3 can be integrated into many of the most popular microscopes from major manufacturers that have anaccessible epi-fluorescent port available. By using this, it is possible to combine illumination for visualisation andphotostimulation simultaneously.

Dimensions in mm [inches]

AMosaic 3 Head

Mosaic 3 uses themicroscope’s epi-fluoresecent
port.

Power Density and Field of Illumination•4 5A

Large field models
33

B

Provides illumination to fill most of the microscope field and matches the FOV of an sCMOS
camera e.g Andor ZL41 Cell and Sona Extreme, and larger EMCCD formats e.g. iXon 888.
Large field models are commonly used for applications such as optogenetics where lower
power densities are delivered by LEDs.

Mosaic 3Small field models
33Provides illumination within a smaller field. Matches the iXon 897 EMCCD camera series.
Small field models are suited to applications including bleaching and uncaging where higher
power densities, delivered by lasers, are required.

Please enquire about specific microscope compatibility and optical configurations
possible.

45

Introduction | Features | Specifications & Ordering

Technical Specifications

Choosing your Mosaic 3 System•2An overview of the building blocks required to configure a Mosaic 3 system is shown below:

Optical Specifications

Please discuss your exact application requirements with your customer representative.

Transmission Range

360 nm to 800 nm (minimum 5% at all wavelengths)

Maximum Exposure Time

200 seconds and above - dependent on trigger mode

Minimum Sequence Mode Latency

200 µsExternal Trigger Rising Edge to Exposure Delay< 200 µs

Minimum Exposure Time Accuracy
Minimum Exposure Time Jitter+/- 1 µs for all defined exposure times

Confirm your microscope type
The Mosaic 3 suits many microscopes makes and models.
Please specify the microscope details in case there are any
requirements specific to your microscope model.

AMicroscope
Type+/- 0.2 µs

Make

Modele.g. Leica, Nikon,Olympus, ZeissYour specificmodelIllumination Sources
Mosaic 3 Illumination Ports

Select your required field of illumination

Up to 2 ports on LHS for: two lasers/ one laser, one LED / two LEDs.•6Description

High Pressure Mercury Lamp XLED LED Light Source
CoolLED e.g pE300, pE100, pE400, pE800 LED

Lamp Sources

405 and 445 High power Mosaic diode lasers
Andor HLE (High Power Laser engine, for Dragonfly)
Other - Please enquire

Laser Sources

BLarge: For large format sensors e.g. iXon Ultra 888, Neo/
Zyla or lower power density applications.

Field ofIllumination

Small: For small format sensors e.g. iXon Ultra 897 or
higher power density applications e.g. bleaching.

Software Compatibility
Select the required Mosaic 3 light sources

Mosaic may be controlled via a range of 3rd party software, as well as by Andor’s dedicated iQ software forphotostimulation.
Andor Mosaic SDKAndor iQ
3rd Party Support

OtherThe following options are available to perform thephotostimulation illumination:

A software development kit that allows you to control the Mosaic 3. Available for Windows (8,8.1 and 10) and Linux. Compatible with C/C++, LabView and Matlab.
Compatible with iQ 2.0 onwards in Single Shot mode.

CMetaMorph MM7 version 7.7.1 onwards and NX version 2.5.275.0: in Single Shot mode only.
NIS-elements version 4.20 onwards: single shot mode (and some additional sequencing
functions).
Mosaic control and targeted sequencing supported under MicroManager “projection” interface.

LED

Other405 and 445 nm
High power Mosaic
Lasers.

LED options e.g.
CoolLED pE100,pE300, pE400,pE4000, pE800

Arc lamps(variousoptions)

HLE (High power laser
engine for Dragonfly)

Integrate with almost any other software using iQ’s virtual camera feature. Continue using
normal acquistion software with photostimulation controlled via iQ.

Select the Epi light sources (Inverted model only)

Trigger Modes
Mirror On

Leave the selected mask on (image exposed) indefinitely.

Mirror Off

All DMD Mirrors ‘Off’ indefinitely.

ExposeExpose the selected mask for a defined exposure time.

Sequence Start•1

Trigger a predefined sequence of masks.

External Expose•1

Use another device to trigger exposure of the selected mask for a defined exposure time.

External Bulb Mode

External Sequence Start

6Mosaic 3Light Source

Lasers•1

Use another device to trigger exposure of the mask on the rising edge of the trigger signal andending on the falling edge.
Use another device to trigger a predefined sequence of masks.

D

EPI Light
Source

The following light sources are available for the Epi input ofInverted models:
Microscope Specific Epi lamp and 3-line LED options.

Choose the required filter setsA wide range of excitation filters and beamsplitter options areavailable to suit your application.

E
Filter Sets

These enable % split and wavelength based split between theobservation and photostimulation wavelengths.

7

Order Today
Need more information? At Andor we are committed to finding the correct
solution for you. With a dedicated team of technical advisors, we are able tooffer you one-to-one guidance and technical support on all Andor products.
For a full listing of our local sales offices, please see:andor.oxinst.com/contact

Our regional headquarters are:Europe					JapanBelfast, Northern Ireland 			Tokyo
Phone +44 (28) 9023 7126			
Phone +81 (3) 6744 4703
Fax +44 (28) 9031 0792			
Fax +81 (3) 3446 8320
North America				China
Concord, MA, USA 				Beijing | Shanghai | Guangzhou
Phone +1 (860) 290 9211			
Phone +86 (400) 678 0609
Fax +1 (860) 290 9566			
Fax +86 (10) 5884 7901
Product Compliance Information
Regulatory Compliance

Footnotes: Specifications are subject to change without notice• EU EMC/ LV/ RoHS2 Directives• Safety-tested under IEC CB Scheme toIEC 61010-1• Laser safety compliant for IEC 60825-1
and CDRH• RoHS-compliant

2. Figures are typical unless otherwise stated.

External Power Supply Compliance
•
••
•
•

UL-certified for U.S. and Canada
U.S. FCC MarkJapan PSE compliant
Korea KC Mark
Taiwan BSMI Mark

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Minimum Computer Requirements:• Operating System: Windows 8.1, 10,and 11 32/64-bit.
• Sandy Bridge i5 Processor or better• USB 3.0 Port or PCI Express x4 slot
• RAM: OS dependent, minimum 4GB
recommended for fast streaming
PC Communications• USB 3.0 (or slower USB 2.0)
• Optional PCIe x1 card supplied toprovide a USB 3.0 port if needed•3Laser Safety
Laser radiation is only present in Mosaic
systems fitted with a laser. Some Mosaic
systems use non laser-based light sources.

1. Currently available through self-integration using Mosaic 3 SDK.
3. Applies if fitted with a laser source.
4. Field number (figures are typical and subject to microscope make and model)
5. Mosaic 3 for inverted microscopes:• Large FOI: 18 to 22 mm (microscope dependent)• Small FOI: 5.8 to 11 mm (microscope dependent)
6. Dependent on Mosaic 3 model.

Mains Supply Requirements• 100 - 240 VAC, 50/60 Hz 1 A max. steadystatePower Consumption• DMD Head with External Power Supply(Typ. & Max.): 6W• DMD Head Only (Typ. & Max.): 4.8W

This product may be supplied with a variety of laser
combinations and output powers at the microscope stage.
The following two labels represent the extremes:• 405 nm Laser: 4.1W
Environmental Requirements• Operating Temperature: 10°C to 30°C
ambient• Relative Humidity: < 70% (non-condensing)• Storage Temperature: -20°C to 70°C

MMosaicSS 0924 R1