Echo® Software Applications
INTUITIVE PROTOCOL DESIGN FOR ECHO LIQUID HANDLERS

Echo®
BROCHURE
Version 2.1 | MAY 2017

LABCYTE INC.
170 Rose Orchard Way
San Jose, CA 95134
USAToll-free: +1 877 742−6548 | Fax: +1 408 747−2010© 2017 LABCYTE INC. All rights reserved.

BROCHURE | ECHO® SOFTWARE APPLICATIONSECHO® ARRAY MAKER

Echo® Software Applications
Echo Software Applications use familiar terminology, interactive graphics and wizards to provide a research-friendly environment for protocol
creation and editing. This drastically reduces the learning curve and hands-on time. Echo applications offer an array of features to maximize theutility, throughput and precision of Echo liquid handlers. All protocols can be simulated before running live to validate each step.

} Echo® Array Maker
Acoustically transfer samples and reagents from any well to anywhere
Echo liquid handlers have the ability to transfer samples and reagents from microplates to custom
patterns in various formats. Echo Array Maker software provides an environment to graphicallydesign custom patterns and formats as well as the transfer protocols that utilize them.

Monitor sample integrity
Create custom destination formats
for Protein Crystallography, MALDI,
and more

FIGURE 1: Pattern Editor dialog box
used to create a pattern to add to thePattern Library. Patterns are created forspecific array types. Pattern dimensions
are limited by the array dimensions forwhich they were created.

Save and share patterns acrossEcho systems
Transfer samples to multiple arrays,
multiple patterns within arrays, andmultiple spots within patterns
Import pick lists to dictate whichsamples are transferred to which
pattern and spot
Produce output files in .CSV, .TXT,
and .GAL formats

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FIGURE 2: Echo Destination Array Editor
dialog box allows the user to define thedestination arrays and patterns for theselected Labware.

ECHO® CHERRY PICKTransfer Any Volume from Any Source Well to Any Destination Well} Echo® Cherry Pick
File-driven transfers from any well to any well
Echo Cherry Pick software uses information in pick lists to transfer from any source well to any
destination well.

FIGURE 3: Import Pick List dialog box isused to define pick lists for the protocol.

Rapid hit picking, sample
pooling and more
Automatic or explicit determinationof which destination plates andwells to use
Customizable output files can besaved in XML, CSV, or TXT format
Custom information can be passed
from pick lists to output files for
LIMS tracking
Import pick lists without
rearranging files
Use a second pick list to transfer
controls to all assay plates
Visually preview each transfer

FIGURE 4: Main screen in Echo Cherry
Pick application with the Pick List tab
selected in the Protocol tab.

Use one protocol to process any
number of pick lists3BROCHURE | ECHO® SOFTWARE APPLICATIONSECHO® COMBINATION SCREEN

Combine Curves of Any Format in 2D and 3D} Echo® Combination Screen
Design complex combination screening layouts
With combination screening the management of pick lists, transfer maps, and plate handling
protocols becomes significantly more complex. Echo Combination Screen software provides agraphical interface to visually combine dose-response curves, controls, and single concentrationtransfers into combination screening protocols for the Echo Liquid Handler.

Combine curves of anyformat in 2D and 3DDesign and save concentration
curve layouts for use acrossprotocols and systems
Curves are automatically generated
from common input information:
starting concentration, dilution
factor, concentration points,
number of controls, and DMSO
concentration limits
Apply multiple curves to platelayouts in row-wise or column-wiseorientations with wrapping andreplicates
Use pick lists to map samples tocurves in the final combination
assay layout

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FIGURE 5: Concentration Curve
dialog boxes and Simulator windowthat shows an animation of fluid
transfer for the selected protocol.

ECHO® DOSE-RESPONSE

Better Dose Titration Results} Echo® Dose-Response
Eliminate carryover and improve results with Direct Dilution™
With tip-based liquid handling, dose-response curves are assembled using serial dilution which
can lead to inaccurate assessments of drug potency as a result of sample carryover. Acoustic,
non-contact, transfer with Echo liquid handers eliminates tips, carryover and dilution errors. Echo
Dose-Response simplifies protocol creation for direct dilution using the Echo liquid handler with awizard-based interface.

Reliable IC50 determinationAssemble dose-response curves by directly transferring different sub-microliter
volumes from one or more stock concentrations to achieve the desired finalconcentration at each point

Visualization of curves layouts,
starting concentrations, final
concentrations and normalization
requirements
Produce row-wise and column-wise
dilutions
Replicate curves in adjacent wells,sequentially or across plates
Automated normalization
Import a pick list to identify
samples for dilutionsfer 1.5 µL of each dilution

75 nL

25 nL

7.5 nL

2.5 nL

Different volumes of the samestock solution are transferred DIRECTLY
to the assay plate
to generate the same final concentrations

StockSolution
10 mM

say plate for total assay volume of 20 µL

75 µM

10% DMSO

25 µM

7.5 µM

2.5 µMDMSO % in Assay = 0.75% After Backfill to 75 nL Total DMSOFIGURE 6: Echo Direct Dilution™ workflow.

5BROCHURE | ECHO® SOFTWARE APPLICATIONSECHO® PLATE AUDIT

Acoustic Validation of Sample Libraries} Echo® Plate Audit
Analyze and monitor sample libraries with acoustic analysis
To deliver superior accuracy and precision, Echo liquid handlers acoustically measure thecharacteristics of a sample or reagent prior to transfer. Echo Plate Audit uses the same acoustic
analysis to visualize, track and compare the characteristics of samples within or across plates in alibrary over time.

FIGURE 7: Acoustic analysis of sample
properties to validate and monitor
sample libraries.

Monitor sample integrity
Assess the composition and volumeof fluid in every well
Visually identify the changes in wellcomposition or volume over time

Fluid Height
VolumeFluid Composition

Use custom rules to declare plates
as passing or failing
Generate pick lists based on
analysis results to select wells fortransfer or replenishment
Import passing/failing status into
Tempo™ Automation Control
Software to automatically qualify
plates for runs on the Access™
Laboratory Workstation

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FIGURE 8: Data Matrix tab displays thevalues for the survey data and allows
the user to filter the survey data byplate type, fluid type, fluid volume, fluidheight, and fluid composition.

ECHO® PLATE REFORMAT

Graphical Mapping of Samples to Any Assay Plate Layout} Echo® Plate Reformat
Design, develop, and assemble assaysEcho Plate Reformat software offers a variety of transfer functions to design custom layouts.
Resulting protocols can be used to assemble assays or reformat and replicate screening libraries.

FIGURE 9: Protocol tab showing acomplex assay design protocol.

Simple or complex assay
designManually define transfer regions tocreate custom layouts
Offset transfers to any position in awell
Insert delays between transfers forincubations, settling, etc.
Evenly deplete grouped wells totransfer large volumes of bulk
reagents
Replicate or reformat sample
libraries in 384 and 1536-wellformats to 96, 384, and 1536 well
assay plates

FIGURE 10: Protocol tab showing
compressed libraries interleaved into apattern.

Compress libraries into interleaved
patterns or separate quadrants

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LABCYTE INC.
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USA
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All product names and brands are properties of their respective owners.
© 2017 LABCYTE INC. All rights reserved. Labcyte®, Echo®, MicroClime®, the Labcyte logo, and Access™
are registered trademarks or trademarks of Labcyte Inc., in the U.S. and/or other countries.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

BRH-ESAP-2.1
MAY 2017