Droplet Digital PCR

Preparing the QX ONE™ Droplet Digital™
PCR System for a Run
Quick Start Guide

Powering On the QX ONE Droplet Digital (ddPCR™) System

Powering Off the QX ONE ddPCR System

1 	Press the On/Off rocker switch located on the back, bottom,

1 	
Close out of the QX ONE Software in the touch screen.

left-hand side of the QX ONE ddPCR System.

2 	Turn off the Windows computer.

a. Select the Windows icon in the lower left corner
Note: If power cycling the instrument, wait 3 min before
engaging the rocker switch to the On position.

2 	Press the round Power button centered on the front of theQX ONE ddPCR System (soft switch). This button will flash
green indicating that power is on and that the instrument
is initializing.
3 	Press the Power switch in the right side panel of the touch

screen to power on the computer.
4 	
Wait at least 5 minutes, then launch QX ONE Softwareby double selecting the QX ONE Software icon (shortcut onthe desktop).
5 	Log in with your user name and password. Typically, when

an instrument in first installed, the generic user name isSuperUser and the designated password is Password1.
For additional steps to add/manage additional users, referto the QX ONE Software Standard Edition User Guide(10000148582) or call the Bio-Rad™ Technical Support
department at 1-800-424-6723, option 2.

b. Select the Power icon.

and then select Shut down.

c. Wait until the touch screen has powered off.
Note: Bio-Rad recommends powering off and restarting theinstrument and computer (touch screen) once per week.
Replacing Oil and Waste Bottles
Bottles are prefilled and sealed with instrument-specific caps.
The notched top helps with bottle alignment and insertion. Theradio frequency identification (RFID) label allows the instrument torecognize the correct positioning for each bottle (droplet generation/
droplet reader oil and waste) and monitor the oil level in each bottle.
1 	To release an empty bottle from its position:a. Firmly push the bottle down to the bottom of the slot
and then release. The bottle pops up from the slot.
b. Lift the bottle out of its position.
c. Replace with a new bottle (see step 2).

6 	
Wait until the instrument status changes from Busy to Ready(approximately 2–5 minutes).

Visit bio-rad.com/QXONE for more information.

ENote: If the instrument does not fully initialize after 10 minutesof powering on, close QX ONE Software, power down thecomputer as you would any standard Windows PC, and power
off the QX ONE ddPCR System by pressing the On/Off switch
at the back, bottom, left-hand side of the instrument. Wait 3
min, then repeat steps 1–4.

Leave
empty

P

Important: Do not touch the instrument or touch screen
during the initialization process.

Droplet generation
oil bottles

Droplet reader
oil bottles

Preparing the QX ONE Droplet Digital PCR System for a Run

2 	
To insert a bottle into its corresponding position:a. Turn the bottle upside down so the notched cap
is facing down.

2 	
Locate the red luer port on the bottom left side of the outbox

as shown.

b. Slowly twist the bottle until the notched cap clicks
into place.
c. F irmly push the bottle down to the bottom of the slot
to snap it into place. Once it is secured, pull up on thebottle to verify that the bottle does not easily lift out ofposition and does not twist in place.

101122334542030
4050 mL53 	
Using a twisting motion, attach a 50 ml luer syringe

to the port.
4 	Draw liquid into the syringe, up to the 50 ml point.
5 	Remove the syringe from the port and dispose it using theProject Title: QX ONE Images

Vendor: Troutt Visual Services
required procedurefor biohazardous materials (fluorinatedFile Name: Hotel_4_left_wo/_plates_K
Date: 09/18/19
Proof: 2hydrocarbon waste
stream disposal).

Project Title: QXOne Manual_Version1.1
Vendor: Troutt Visual Services
File Name: 07_50mL_syringe_in
Date: 08/26/20
Proof: 1

Vent Reservoir Maintenance1 	In the QX ONE Software touch screen, select Open Outbox

in the toolbar. The dialog box will appear as shown.

Project Title: QX ONE Images
Vendor: Troutt Visual Services
File Name: 21_QX_ONE_DR_oil_RFID
Date: 12/22/17
Proof: 1

Note: Bio-Rad recommends venting the residual oil reservoir
before the initial run and/or once a week depending on thefrequency of use of the QX ONE ddPCR System. To reduce thecollection of residual oil, remove the waste bottle when theinstrument is not in use and store with the quick connect
ports facing the ceiling.
Refer to the QX ONE Droplet Digital PCR System and QX ONE
Software User Guide (10000116512) for additional details.
Visit bio-rad.com/QXONE for more information.

Project Title: QX ONE Images
BIO-RAD, DDPCR, DROPLET DIGITAL, and QX ONE are trademarks of Bio-Rad
Vendor: Troutt Visual Services
File Name: 18_QX_ONE_DG_oil_topLaboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of theirDate: 03/13/18
respective owner. © 2022 Bio-Rad Laboratories, Inc.
Proof: 3

The QX ONE Droplet Digital PCR System, and the consumables and reagents designed
to work with this system, and/or their use is covered by claims of U.S. patents and/orpending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad
Laboratories, Inc. See bio-rad.com/en-us/trademarks for details. Purchase of theproduct includes a limited, non-transferable right under such intellectual property for useof the product for internal research purposes in the field of digital PCR only. No rights aregranted for diagnostic uses. No rights are granted for use of the product for commercial
applications of any kind, including but not limited to manufacturing, quality control,or commercial services, such as contract services or fee for services. Information
concerning a license for such uses can be obtained from Bio-Rad Laboratories. It is theresponsibility of the purchaser/end user to acquire any additional intellectual property
rights that may be required.

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