Food Testing

CFX Manager Software,
Industrial Diagnostic Edition (IDE)
iQ-Check Prep Sample Setup Protocol
Quick Guide■CFX96 Deep Well
Real-Time System™

C1000 Touch
Thermal Cycler™

C1000 Touch™ Thermal Cycler

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Turn on the CFX96 Touch Deep Well Real-Time PCR System and open CFX Manager
Software, IDE, to launch the IDE Plate Manager window. If necessary, either click the IDE
Plate Manager icon on the toolbar, or go to the menu and select View > IDE Plate Managerend■

Select the test you wish to perform from the list of kits on the left side of the screen■

Select the well(s) for that assay■■■■

Apply sample types to your wells by selecting Positive Control, Negative Control, orUnknown (Easy I, Easy II, or No extraction)

Type or select lot numbers for PCR Kit and/or DNA Extraction Kit

Type or select sample names: select a well to start, enter this well’s name in the Sample
Name box, and press the Enter key. The cursor will move to the next well down
Additional kits can be added by selecting from the list of kits to create new test groups
and repeating the steps
continues

Visit bio-rad.com/foodscience for more information.

CFX Manager Software, Industrial Diagnostic Edition (IDE): iQ-Check Prep Sample Setup Protocol Quick Guide■

Click Send to iQ-Check Prep■

Turn on the iQ-Check Prep System and open the iQ-Check Prep operating software■

Press or click the Start button

iQ-Check Prep
™

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The daily maintenance is required if it has not been performed yet.
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Load the appropriate worklist
Carefully follow the loading instructions on the screen in the exact order mentioned:
PCR plate(s), lysis reagent(s), PCR reagents, sample deep well plate, extraction deep wellplate(s), and transfer well plate500 µl■

After the enrichment step, transfer 500 μl (minimum 150 μl to maximum 1,000 µl) ofsample into a deep well plateiQ-Check Prep™

Avoid shaking the sample bag to prevent collecting large fragments of food debris.
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Load the sample deep well plate into the iQ-Check Prep System and start the run!
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Remove the PCR plate and seal it properly using optical caps. (If using optical film, a plate
sealer is recommended)
Scan the barcode of the PCR plate into CFX Manager Software, IDE, using the LoadPlate File option from the Barcode menu■

Place the PCR plate into the thermal cycler■Start the amplification by clicking Run

CFX96 Deep Well
Real-Time System™

C1000 TouchThermal Cycler™

C1000 Touch™ Thermal Cycler

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For detailed instructions, review the kit user guide and instrument user manual.

Visit bio-rad.com/foodscience for more information.
BIO-RAD is a trademark of Bio-Rad Laboratories, Inc. IQ-CHECK is a trademark of Bio-Rad Europe GmbH in certain jurisdictions.
All trademarks used herein are the property of their respective owner.

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