Food, Beverage, and Water Safety Testing

CFX Opus 96 and CFX Opus Deepwell
Real-Time PCR Systems for Food,
Beverage, and Water Safety Testing

The Power of PCR
The CFX Opus 96 and CFX Opus Deepwell
Real-Time PCR Systems bring modern
quantitative PCR (qPCR) to your food, beverage,and water testing needs. The CFX Opus
Deepwell System offers precise discriminationof up to five targets in large-volume reactions.
The system incorporates industry-leading
technology to provide a robust and easy-to-use
real-time PCR instrument for reliable results.
CFX Manager Software, Industrial Diagnostic
Edition (IDE), allows for easy sample setup,
result analysis, and bidirectional laboratory
information management system (LIMS)
connection when running the Bio-Rad suite offood, beverage, and water testing PCR assays.
Benefits■ Use reaction volumes ≤10 µl, depending on
the application
■

■

■

■

 ith CFX Opus Deepwell System, run one
Wof the largest qPCR reaction volumes in theindustry, up to 125 µl

Specifications
SystemSample capacity

96 wells

Sample size
CFX Opus 96	1–50 µl(10–50 µl recommended)
CFX Opus Deepwell 	1–125 µl(10–125 µl recommended)Dimensions (W x D x H)		33 x 56 x 36 cm(13 x 22 x 14 in.)

Weight22 kg (48 lb)

Touch-screen user interface	Adjustable, with angle ofrotation 12–55ºCommunications	USB 2.0 or above, Ethernet,
wireless network (WiFi)
Electrical approvals

IEC, CE

Operating system

Windows 10 or 11 IoT

Optical Detection System
Excitation
6 filtered LEDs
Detection

6 filtered photodiodes

Range of excitation/emission wavelengths

450–730 nm

Scan time
All channels
Single channel

2 sec
3 sec

 ontrol up to 4 CFX instruments with CFXCManager Software, IDE (or Security Edition),for Windows 10 or 11 (64-bit)

Dynamic range

10 orders of magnitude

Email your completed run

Multiplex analysis

 up unique user names, with optional
Setpasswords, to easily organize and findyour protocols and experiments

Visit bio-rad.com/FoodScience for more information.

Sensitivity	Detects 1 copy of target
sequence in humangenomic DNA
Up to 5 targets per wellcontinues

CFX Opus 96 and CFX Opus Deepwell Real-Time PCR Systems for Food, Beverage, and Water Safety TestingSpecifications

Ordering Information

96-Well Reaction Block
Sample block typeAll software for Windows unless otherwise noted.
Fixed 96-well sample block

Heating and cooling method Peltier
Lid heating

30–110ºC

Temperature range

4–100ºC

Gradient
Operational range
Programmable span

30–100ºC
1–24ºCCFX Opus 96 System
Maximum ramp rate

5ºC/sec

Average ramp rate

3.3ºC/sec

Thermal accuracy±0.2ºC

Thermal uniformity	±0.3ºC, measured 10 sec after the block reaches
the target temperature
CFX Opus Deepwell System
Maximum ramp rate
2.5ºC/sec
Average ramp rate

2ºC/sec

Thermal accuracy±0.2ºC

Thermal uniformity	±0.4ºC, measured 10 sec after the block reaches
the target temperature
CFX Manager Software, IDE
iQ-Check™ Real-Time
CFX Opus Deepwell System only
PCR Kits
Operating system	Windows 10 or 11 (64-bit)
MemoryMinimum of 1 GB

Data analysis modes

PCR quantification with standard curve		

Melt curve analysis		Gene expression analysis by relative quantity (∆Cq)
or normalized expression (∆∆Cq)
		Multiple-file gene expression analysis for comparisonof an unlimited number of quantification cycle (Cq) values		

Allelic discrimination		Endpoint analysis		Data analysis options include bar charts, box-andwhisker plots, dot plots, clustergrams, scatter plots,and volcano plots				

Statistics include t-test and analysis ofvariance (ANOVA)

Catalog #Description

17007992	CFX Opus 96 Real-Time PCR System, includes power cord,USB cable, and Ethernet cable; does not include CFX Software orWiFi adaptor
17007991	
CFX Opus Deepwell Real-Time PCR System, includes
power cord, USB cable, and Ethernet cable; does not include
CFX Software or WiFi adaptor
3593893
CFX Manager Software, Industrial Diagnostic Edition (IDE)
1845098
CFX Qualification Plate, 96-well
HSP9655XTU	Hard-Shell™ 96-Well PCR Plates, low profile, thin wall, skirted,
white shell/white wells
HSP9955XTU	Hard-Shell 96-Well PCR Plates, barcoded, low profile, thin wall,skirted, white shell/white wells
MLL9651XTU
Multiplate 96-Well PCR Plates, low profile, unskirted
MSB1001XTU
Microseal™ 'B' PCR Plate Sealing Film
TLS0851XTU
Low-Profile 8-Tube PCR Strips without Caps, 0.2 ml
TCS0803XTU
Optical Flat 8-Cap Strips, for 0.2 ml PCR tubes
iQ-Check Kits
12010806
iQ-Check Aspergillus PCR Detection Kit, 96 reactions
3578135
iQ-Check Campylobacter PCR Detection Kit, 96 reactions
3578137
iQ-Check Cronobacter PCR Detection Kit, 96 reactions
12003068
iQ-Check Enterobacteriaceae PCR Detection Kit, 96 reactions
3578114
iQ-Check E.coli O157:H7 PCR Detection Kit, 96 reactions
3578124	iQ-Check Listeria monocytogenes II PCR Detection Kit,
96 reactions
3578113
iQ-Check Listeria spp. PCR Detection Kit, 96 reactions
3578123
iQ-Check Salmonella II PCR Detection Kit, 96 reactions
3578142
iQ-Check S. Enteritidis PCR Detection Kit, 96 reactions
12004306
iQ-Check S. Typhimurium PCR Detection Kit, 96 reactions
3578139
iQ-Check STEC VirX PCR Detection Kit, 96 reactions
12013174
iQ-Check STEC SerO II PCR Detection Kit, 32 reactions
12006574
iQ-Check Vibrio PCR Detection Kit, 96 reactions
3578104	
iQ-Check Screen Legionella spp. Real-Time PCR
Detection Kit, 96 reactions
3578105	
iQ-Check Screen L. pneumophila Real-Time PCR
Detection Kit, 96 reactions
3578102	
iQ-Check Quanti Legionella spp. Real-Time PCR
Quantification Kit, 96 reactions
3578103	
iQ-Check Quanti L. pneumophila Real-Time PCR
Quantification Kit, 96 reactions
3548101
VINEO™ Brettanomytest PCR Kit, 96 reactions

Data export	Save, copy, and print all graphs and spreadsheetsfrom right-click menu

Visit bio-rad.com/FoodScience for more information.		

BIO-RAD, HARD-SHELL, and MICROSEAL are trademarks of Bio-Rad Laboratories, Inc.
in certain jurisdictions. IQ-CHECK and VINEO are trademarks of Bio-Rad Europe GmbH incertain jurisdictions. All trademarks used herein are the property of their respective owner.
© 2023 Bio-Rad Laboratories, Inc.

Export specified data in multiple formats		Copy and paste into a Microsoft Excel, Word,or PowerPoint file		Print directly or save as PDFs customizable reports
containing run settings, data graphs, and spreadsheets		

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