Real-Time PCR

CFX Duet Real-Time
PCR System

Powerful Two-Color Genomics Analysis,
Accessible for Most Laboratories
The CFX Duet Real-Time PCR System makes
uniform and precise quantitative PCR (qPCR)
accessible for any lab. The CFX Duet provides
the thermal performance and the proprietary,
accurate optical shuttle system of our premium
CFX Opus Real-Time PCR Systems
for customers that run only singleplex orduplex assays.
The CFX Duet System utilizes CFX Maestro
Software through an attached computer as its
control and analysis system. The economicaluser can quickly get started with geneexpression, genotyping, thermal gradient
optimization, quantification, and even proteincharacterization using fluorescence resonanceenergy transfer (FRET). The two-color CFX
Duet System is factory calibrated for common
dyes such as SYBR® Green and EvaGreen®.
This system is not compatible with the Br.io
cloud platform.
Benefits■ 
Easy entry point into basic qPCR with2-color system■■■ o calibration or passive reference
Ndyes required ersatile capabilities for essential molecularV
biology and protein characterization
 dditional FRET LED channel, enablingAprotein melt analysis

Visit bio-rad.com/CFXDuet for more information.

■■■

 ime-saving experiment optimization withT
thermal gradient feature ptimal thermal performance equivalent toO
the premium CFX Opus Systems
 ontinued support for and control of up to 4
C
CFX instruments with CFX Maestro Software,and Security Edition

Specifications
SystemSample block type

Fixed

Sample capacity

96 wells

Sample size	1–50 µl (10–50 µl
recommended)
Dimensions (W x D x H)		33 x 56 x 36 cm(13 x 22 x 14 in.)

Weight22 kg (48 lb)

Communications	USB 2.0 or above
Electrical approvals

IEC, CE

Optical Detection System
Excitation
3 filtered LEDs
Detection

3 filtered photodiodes

Range of excitation/emission wavelengths

450–580 nm

Scan time
All channels
Single channel

12 sec
3 sec

Dynamic range
10 orders of magnitude
Sensitivity	Detects 1 copy of target
sequence in humangenomic DNA
Multiplex analysis

Up to 2 targets per wellcontinues

CFX Duet Real-Time PCR System

Specifications

Ordering Information

96-Well Reaction Block
Heating and cooling method Peltier
Lid heating
30–110ºC
Temperature range
4–100ºC
Maximum ramp rate
5ºC/secAverage ramp rate
3.3ºC/sec
Thermal accuracy±0.2ºC
Thermal uniformity	±0.3ºC (max – min ≤ 0.6ºC), measured 10 sec after
the block reaches target temperature
Gradient
Operational range
30–100ºC
Programmable span
1–24ºC

Catalog #CFX Maestro Data Management and Analysis Software
Operating system	Windows 10 (64-bit)
MemoryMinimum of 1 GB

Data analysis modes

PCR quantification with standard curve		

Melt curve analysis		Gene expression analysis by relative quantity (∆Cq)
or normalized expression (∆∆Cq)
		Multiple-file gene expression analysis for comparisonof an unlimited number of quantification cycle (Cq) values		

Allelic discrimination		Endpoint analysis		Data analysis options include bar charts, box andwhisker plots, dot plots, clustergrams, scatter plots,
and volcano plots				

Statistics include t-test and analysis ofvariance (ANOVA)

Data export	Save, copy, and print all graphs and spreadsheetsfrom right-click menu		

Export specified data in multiple formats		Copy and paste into a Microsoft Excel, Word,
or PowerPoint file		Print directly or save as PDFs customizable reports
containing run settings, data graphs, and spreadsheets		

Export to RDML

Image export	Export images at any pixel size and at a resolution
up to 600 dpi		

Save images as .bmp, .jpg, or .png files

Description

Instrument
12016265

 FX Duet Real-Time PCR System, includes power cord,C
USB cable

SoftwareAll software for Windows unless otherwise noted.
12013758
CFX Maestro Software, includes USB installation drive, video
quick guides, PDF instruction manual
12012832
CFX Maestro Software, Security Edition, 1 license
12013028
CFX Maestro Software, Security Edition, 5 licenses
12012833
CFX Maestro Software, Russian Edition, 1 license
12012834CFX Maestro Software, Chinese Edition, 1 license
1845025
Precision Melt Analysis Software, includes 2 user licenses,
installation CD, 2 HASP HL keys, melt calibration kit
Consumables
HSP9655
Hard-Shell™ 96-Well PCR Plates, pkg of 50, low profile, thin wall,skirted, white shell/white wells
HSP9601
Hard-Shell 96-Well PCR Plates, pkg of 50, low profile, thin wall,
skirted, white shell/clear wells
MSB1001
Microseal™ 'B' PCR Plate Sealing Film, pkg of 100, optically clear
seals for PCR plates
TLS0851
0.2 ml 8-Tube PCR Strips without Caps, low profile, white
TLS0801
0.2 ml 8-Tube PCR Strips without Caps, low profile, clear
TCS0803
0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear
Reagents
1845098
CFX Qualification Plate, 96-well12010176
Reliance One-Step Multiplex RT-qPCR Supermix, 1 ml (1 x 1 ml),
200 x 20 μl reactions
1725270
SsoAdvanced Universal SYBR® Green Supermix, 2 ml (2 x 1 ml),
200 x 20 μl reactions
1725016
SsoAdvanced Universal Inhibitor-Tolerant SYBR ® GreenSupermix, 2 ml (2 x 1 ml), 200 x 20 μl reactions
1725095
SingleShot SYBR ® Green One-Step Kit for Cell Lysis andRT-qPCR, 100 x 50 μl reactions
17005726
SEQuoia Complete Stranded RNA Library Prep Kit, 24 reactions
12011928
SEQuoia Dual Indexed Primers Set, 12 vials of unique dual
indexed primers, 96 reactions

Search CFX qPCR Systems in the Apple App Store and Android
Play Store for an augmented reality tour. Contact your local sales
representative for an in-person demonstration.
Visit bio-rad.com/CFXDuet for more information.
BIO-RAD, HARD-SHELL, and MICROSEAL are trademarks of Bio-Rad Laboratories, Inc.
in certain jurisdictions. All trademarks used herein are the property of their respective owner.
© 2023 Bio-Rad Laboratories, Inc.
EvaGreen is a trademark of Biotium, Inc.
SYBR is a trademark of Thermo Fisher Scientific Inc. Bio-Rad Laboratories, Inc. is licensed
by Thermo Fisher Scientific Inc. to sell reagents containing SYBR Green I for use in real-time
PCR, for research purposes only.

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