Amplification: Thermal Cyclers

1000-Series Thermal Cyclers

ADVANCING PCR TOGETHER

Take control of your experiments. Bio-Rad’s 1000-series thermal cycling
platform gives you the power to do the experiments you want to do, theway you want to do them. The high-performance 1000-series platform canbe configured to meet any researcher’s unique needs. Choose the C1000
Touch™ Thermal Cycler, the S1000™ Thermal Cycler, or a combination of both.
Choose from multiple interchangeable reaction modules, including two for

1 | BIO-RAD LABORATORIES, INC.

1000-SERIESTHERMAL CYCLERS

real-time PCR. Use the intuitive touch-screen interface on the C1000 Touch
Cycler, optimize PCR assays using the gradient feature, and transport
your files and data with a USB flash drive. No matter how you configure
your 1000-series system, you’ll get superior thermal performance in a
simple-to-use, dependable instrument that delivers accurate results andthe flexibility to expand as your research grows.

BIO-RAD LABORATORIES, INC. | 2

FASTER, SMARTER, EASIER PCRPCR Faster

Rapid Arrival at Target Temperature

If you’re like most researchers, you want to get results faster.
Many factors contribute to the overall time it takes to get PCR
results: the time to design, program, and optimize a protocol,
the time to run the protocol, and the time to analyze the results.
Bio-Rad has developed a complete thermal cycling system thatwill speed every step of your PCR process to give you what’s
really important — a shorter time from setup to results.

A key component of overall protocol run time is the time to reach target
temperature, which is determined by the average ramp rate and the time
for the sample block to reach thermal uniformity. Maximum ramp rateis less important because it can fluctuate significantly during the ramp.
The C1000 Touch and S1000 Thermal Cyclers’ tight temperature control
produces high average ramp rates and tight uniformity during ramping
to yield fast time to target temperature and faster protocol run times.

Protocol autowriter — generates an optimal protocol basedon your polymerase, primers, and product length

The time to reach target temperature, which comprises the averageramp rate and the settling time (the time it takes to reach thermal
uniformity), is the key factor determining how fast a thermal cycler can
run a given PCR protocol. Average ramp rate is a better indicator ofa cycler’s capabilities than maximum ramp rate because the latter isgenerally not maintained throughout a temperature step. The averageramp rate of 1000-series cyclers, combined with a 10 sec settling time,allows fast run times while maintaining excellent thermal accuracy
and uniformity.

Thermal gradient feature — identifies optimal annealing
temperature in a single run
Reduced-mass honeycomb sample block — fast ramping
and settling produce the shortest time to target temperature
Intuitive software — makes programming faster and easierfor PCR and real-time PCRSuperior Uniformity

Performance

The overall run time of a PCR reaction depends on protocol design,
Precision of the temperature steps is critical for the rate andenzyme type, and the thermal capabilities of the thermal cycler.
efficiency of PCR. To obtain reliable, consistent results, it
1000-series thermal cyclers deliver premium thermal performance foris important that all wells maintain the proper temperature
reproducible results and fast run times.
throughout each incubation step. This is especially critical for
precise quantification in real-time PCR. 1000-series thermal
cyclers have six independently controlled thermal electricmodules (TEs), the heating and cooling elements of the thermal
cycler, to maintain tight temperature uniformity at all points
during a run — even during ramping.

90
Temperature, °C

85
80
75
70
65
60
55

The patented* reduced-mass honeycomb sample block heats and cools more
quickly than standard blocks, so average ramp rates are increased and overall
run times are reduced.
* U.S. patent 7,632,464.

3 | BIO-RAD LABORATORIES, INC.

0

20

40

60

80 100 120 140 160 180 200
Time, sec

Superior uniformity with rapid arrival at target temperature. 1000-series
thermal cyclers exhibit high average ramp rates, rapid settling time, and tight thermal
uniformity throughout the ramp. This graph shows the temperature measured byprobes in 15 wells across a sample block. The traces are nearly indistinguishable dueto the tight uniformity. Note the consistent high average ramp rate throughout heating
and cooling.

1000-SERIESTHERMAL CYCLERS

PCR Smarter

PCR Easier

Smarter means no hassles. Smarter means giving you thefreedom to do the things you want to do. And most of all,smarter means trustworthy and dependable. Bio‑Rad’s
1000-series instruments simplify your work with tools accessedwith an easy-to-use interface. 1000-series instruments are
dependable and long-lasting, and they generate data you
can trust.

Since PCR is central to genomics research, you need aflexible platform that can adapt as your research evolves.
Bio-Rad’s 1000-series cycling platform offers two chassis
options, six interchangeable reaction modules, and simple
expansion capabilities, so you can create a setup that cangrow with you and continue to meet your needs.

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 asily interchangeable reaction modules let you changeEformats quickly without tools
 btain better results with 6 TEs that produce precise,O
uniform temperatures
 -ring hermetic seal protects TEs from condensation,Omaximizing their life eal-time PCR detection system uses long-lasting solid-stateRlight-emitting diodes (LEDs) and photodiodes

Thermal Gradient for Easy Protocol Optimization

First, choose a chassis: the feature-rich C1000 Touch Thermal
Cycler, the streamlined, equally powerful S1000 Thermal Cycler,or a combination of the two. Next, choose one or more reaction
modules: 96-well fast, 96–deep well, 384-well, or independently
controlled dual 48/48-well fast format, or a six-channel 96-wellor five-channel 384-well optical reaction module for real-time
PCR. Adding instruments to increase throughput is as easy as
connecting a USB cable — up to 32 thermal cyclers can becontrolled by one computer. You can even connect thermal
cyclers and real-time PCR detection systems, run different
protocols, and start experiments at different times.

The programmable temperature gradient allows you to identify
the optimal annealing temperature in a single PCR experimentby letting you test eight incubation temperatures at once.
Optimizing reaction conditions results in higher specificity andhigher yields.
The 1000-series gradient feature uses dynamic ramping,which means the temperature gradient forms during ramping
and all wells come to the designated temperature at thesame time. As a result, the incubation period is the same forall samples, which is crucial for transfer of gradient tonongradient protocols.

104

10

20
0

10

20

30

1

40

103

3

4

5

6

E = 98.7%

25

102

20
15

0

10

20

30

1

40

30

103

25
Cq

104

102
10

2

30
Cq

RFU
RFU

56°C

30
25

104

10

E = 68.3%

35

2

10

62°C

40

103
Cq

RFU

67°C

2

3

4

5

6

E = 93.6%

20
15

0

10

20
Cycles

30

40

1

2
3
4
5
log starting quantity

6

Thermal gradient experimentfor optimizing annealing
temperature. A tenfold dilution
series (10 6 –10 copies) of plasmid
containing GAPDH template wasamplified in the presence of SYBR®
Green Dye using a protocol with anannealing thermal gradient ranging
from 55 to 68°C. Results arepresented for three temperatures,showing 62°C as the optimal inthis case, with early Cq values
and the highest standard curve
efficiency. Cq, quantification cycle;
RFU, relative fluorescence units.

96-well fast reaction module
96–deep well reaction module■■ 384-well reaction module
■■
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Dual 48/48-well fast reaction module

CFX96™ Optical Reaction Module
CFX96 Deep Well Optical Reaction Module■■ CFX384™ Optical Reaction Module■■■■

BIO-RAD LABORATORIES, INC. | 4

THERMAL CYCLERS
C1000 Touch Thermal Cycler — A Premium Instrument for PCR
The C1000 Touch Cycler is the flagship of the 1000-series thermal
cycling platform, offering unmatched performance for fast, reliable
results and a state-of-the-art interface with new ways to optimize
protocols and monitor runs. The C1000 Touch Cycler offers all thepowerful features of the 1000-series platform, so you can:
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R
 un real-time PCR experiments using the CFX96, CFX96 Deep Well,
or CFX384 Optical Reaction ModuleQ uickly optimize reactions using the protocol autowriter ave time by creating and viewing protocols using the large color
S
touch-screen display and intuitive graphical programmingG et answers quickly using desktop support, data logging,
and run reports

Optimize reactions using the protocol autowriter.

 ave the convenience of sending an email notification ofH
run completionB ack up your data, and manage and transfer files usinga USB flash driveH ave peace of mind by protecting your files with optionallog-in, restricted user privileges, and secure mode for controlledenvironmentsI ncrease throughput simply and easily by daisy-chaining up to
3 additional cyclers or adding PC control for up to 32 cyclers
Quickly customize run parameters.

Use the Saved Files feature to organize data.

5 | BIO-RAD LABORATORIES, INC.

1000-SERIESTHERMAL CYCLERS

Dual 48/48-Well Fast Reaction Module —It’s Like Having Two Thermal Cyclers in One

S1000 Thermal Cycler —A Great Companion

Bio-Rad offers a gradient-enabled dual reaction module that allows
you to run two independent experiments at the same time. Thisability is valuable for laboratories with multiple users running different
applications on different schedules. A dual thermal gradient isconvenient for protocol optimization, allowing more variables to betested simultaneously.

The S1000 Cycler is just right for researchers who require less — ormore — than what the C1000 Touch Cycler offers. Those who simply
need dependable performance can use the S1000 Cycler as a standalone instrument for PCR. Those who need more than one powerfulcycler can connect up to three S1000 Cyclers to a C1000 Touch Cycler
to form a high-throughput multi-bay instrument.
The S1000 Cycler offers the same thermal performance as
the C1000 Touch Cycler, and also lets you:
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
Programprotocols easily with the semi-graphicaluser interface
Choosea reaction module that suits your needs — dual
48/48-well fast, 96-well fast, 96–deep well, or 384-well format

 et optimal sealing using your favorite vessels and sealers withG
the redesigned fully adjustable heated lid

BIO-RAD LABORATORIES, INC. | 6

REAL-TIME PCR DETECTION SYSTEMS
Precise Detection
The CFX96, CFX96 Deep Well, or CFX384 Optical Reaction Module converts
a C1000 Touch Cycler into a powerful and precise real-time PCR detection
system. Both real-time PCR systems include advanced optical technology
combined with precise thermal control to deliver the most sensitive, reliable
detection in different throughput formats. The accompanying CFX Manager™
Software, which runs on a PC, provides numerous features and tools to simplify
experiment setup and data analysis.
As the optics shuttle of the CFX96 Touch™, CFX96 Touch Deep
Well, or CFX384 Touch™ Real-Time PCR Detection System
travels across the plate, light is focused directly into the centerof each sample well. Side view of the optics shuttle shows thegreen LED firing over a well.

With the CFX96 Touch, CFX96 Touch Deep Well,
and CFX384 Touch Systems you can:
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7 | BIO-RAD LABORATORIES, INC.

 ave reagents and samples by detecting up toS
5 targets per well in the CFX96 Touch or CFX96
Touch Deep Well System or up to 4 targets
per well in the CFX384 Touch System using
long-lasting multicolored LEDs and sensitive
photodiodesT ailor a run to suit your application using multiple
data acquisition modes, including a fast scan for
SYBR® Green and FAM
G et your experiment under way before spendingtime to enter or edit plate information — both
systems automatically collect data from all wellsS ave benchspace with multiple configuration
options, including a stand-alone mode without
a computerG et more accurate gene expression results byusing multiple reference genes and individual
reaction efficiencies for normalizationA nalyze data when and where you want —when a run is finished get email notification
with a data file attached ake decisions sooner about next steps in yourM
experiment by monitoring real-time reactions on
the instrument’s large touch-screen display

1000-SERIESTHERMAL CYCLERS

Excitation

Channel 1 Channel 2
450–490 515–535

Reporter dye:

FAM

HEX

Channel 3
560–590

Channel 4 Channel 5
620–650 672–684

Texas Red

Cy5

Quasar 705

1.00

103
RFU

Normalized absorbance

0.90
0.80
0.70
0.60

102

0.50
0.40
0.30

10

20

0.20

30

40

Cycles0.10

Accurate five-target multiplex gene expression is produced using the precise
optics of the CFX96 Touch System. Human spleen RNA was transcribed into cDNA
using the iScript™ cDNA Synthesis Kit. cDNA (50 ng) was amplified in four replicate
50 μl reactions using five reporter dyes to monitor fluorescence data from five targets:n, FAM/actin (18.02 ± 0.02); n, Cy5/tubulin (20.07 ± 0.03); n, HEX/GAPDH(22.81 ± 0.02); n, Quasar 705/IL-1b (26.39 ± 0.04); n, Texas Red/IL2 (29.17 ± 0.07).
Average Cq values are shown in parentheses. RFU, relative fluorescence units.

0.00
425

450

500

550

600

650

700

750

775

Wavelength, nm

Detection

Channel 1 Channel 2
510–530 560–580

Reporter dye:

FAM

Channel 3
610–650

HEX

Channel 4 Channel 5
675–690 705–730

Texas Red

Cy5

Quasar 705

1.00

Normalized absorbance

0.90
0.80
0.70
0.60
0.50
0.40

RFU

0.30
0.20

102

0.10
0.00
425

450

500

550

600

650

700

750

775

Wavelength, nm

Optimal reporter dye signal collection is achieved using the discrete excitationand detection wavelength ranges of the CFX96 Touch System. Channels 1– 4 are
identical to the CFX384 Touch system.
A

104
104
RFU

18

20

22

24

26

28

30

CyclesPrecise target discrimination results from the tight thermal control of the CFX96
Touch System. One-cycle spacing between Cq values is precisely maintained in aseries of twofold dilutions of human genomic DNA from 120 to 15 ng. IL-1b target was
amplified using a FAM-labeled detection probe with iQ™ Supermix. Graph shows eight
replicates for each dilution with the following average Cq values: 22.15 ± 0.02, 23.14 ±
0.04, 24.28 ± 0.03, 25.41 ± 0.04. RFU, relative fluorescence units.

B

103

103

16

104
102

0

10

20

30

40

C

0

10

20

30

40

D
104

104

103

RFU

RFU

102

103

102

102
0

10

20
Cycles

30

40

0

10

20

30

40

103

102

CyclesBroad linear dynamic range of four-target multiplexing using the CFX384 Touch
System ensures detection of less abundant products. A–D, fluorescence datafrom a series of tenfold dilutions of plasmid DNA (108 –102 copies) amplified using
reporter dyes to monitor four targets: n, FAM/actin; n, HEX/GAPDH; n, Texas Red/
cyclophilin; n, Cy5/tubulin. RFU, relative fluorescence units.

0

10

20

30

40

CyclesExcellent uniformity using the CFX384 Touch System provides reliable results.
IL-1b plasmid template diluted to 105 copies per reaction amplified in the presenceof a FAM-labeled detection probe with iQ Supermix. Graph shows 384 replicates of
5 μl reactions. Average Cq value = 18.11 ± 0.045. RFU, relative fluorescence units.

BIO-RAD LABORATORIES, INC. | 8

A COMPLETE SYSTEM
1000-series cyclers and reaction modules are a powerful platform
for genomics research. Bio-Rad offers a wide selection ofreagents, vessels, and sealers to help you make the most of your
PCR experiments.

Sso7d

Polymerase

RNA Isolation
■■

■■

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 its are designed and formulated to assist in the isolation of highly pureK
and intact RNA from different starting materials
 NA is compatible with a variety of downstream applications
R— Real-time quantitative PCR (qPCR)— Northern blotting— Microarray analysis
DNase treatment ensures genomic DNA removal

Reverse Transcription Reagents■■■■

 ormulated for efficient reverse transcription across a broad
Flinear dynamic range

Bio-Rad’s SsoFast™ qPCR Supermixes utilize patented* Sso7d fusion protein
technology to provide enhanced processivity, speed, and tolerance to PCR
inhibitors. SsoFast EvaGreen Supermix delivers enhanced fluorescence compared
to SYBR® Green. SsoFast Probes Supermix enables robust detection of two different
gene targets under standard or fast cycling conditions.

 otent RNase A inhibitors protect RNA during setup andPreverse transcription■■

Flexible input RNA capacity to suit different experimental needs■■

O ptimized for gene expression analysis using real-time qPCR

Real-Time qPCR Reagents
■■

■■

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 atented* Sso7d fusion enzyme technology delivers higher processivity
Pand inhibitor tolerance
 ntibody-mediated hot-start polymerases enable instant activationAand higher specificity
Choice of fast, standard, or universal cycling conditions
 ormulated for optimal performance on a variety ofFreal-time instruments

Bio-Rad’s broad selection of vessels and sealers for PCR and real-time PCR.

45

PCR Plastic Consumables
Precisely manufactured for optimal fit and cycling performance■■Produced in Class 10,000 or 100,000 cleanroom environment■■

Certified to be free of DNase, RNase, and human genomic DNA■■■■ xtremely uniform wells reduce well-to-well variability inEreal-time PCR
Warp-free Hard-Shell® Plates are designed for optimum performancewith automation* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

40
35
30
Cq

■■

25
20
15
10
5
0
0.0001

0.001

0.01

0.1
1
10
Input RNA, ng/reaction

100

1,000

10,000Maximum efficiency and sensitivity with iScript Reverse Transcription Supermix
for RT-qPCR and SsoFast Probes Supermix. Different amounts of HeLa cell RNA(1 ng–1 pg) were reverse transcribed and one-tenth of resulting cDNA was usedas template to amplify β-actin ( ; ~90 bp) and a-tubulin ( ; ~120 bp) genes in 20 μl
qPCR reactions. Data demonstrate efficient reverse transcription and amplification
across a broad linear dynamic range for reliable gene expression analysis.
Cq, quantification cycle.

9 | BIO-RAD LABORATORIES, INC.

1000-SERIESTHERMAL CYCLERS

Specifications
Thermal Cycler

C1000 Touch

S1000

Input power

Up to 850 W maximum

Up to 700 W maximum

Frequency

50 –60 Hz, single phase50 –60 Hz, single phase

Display

8.5 in. LCD display and touch screen

LCD

Ports

5 USB A, 1 USB B

4 USB A, 1 USB B

Memory 1,000 typical programs onboard;>
unlimited with USB flash drive expansion>1,000 typical programs onboard

Dimensions (W x D x H)

13 x 18 x 8 in.

13 x 18 x 8 in.

Weight

23 lb

23 lb

Temperature control modes

Calculated and block

Calculated and block

Programming options

Step-based graphical and automatic

Text based

Security featuresOptional log-in required mode for regulated environments—

ReportingExportable run logs, system error logs

Yes, if attached to a C1000™ or C1000 Touch Cycler

Onboard software

Windows CE 6.0

No

PC compatibility

Windows XP or higher

Windows XP or higher with C1000 or C1000 Touch Cycler

USB peripheral compatibility

Mouse, USB flash drive, bar code reader

No

Real-time PCR upgrade

CFX96, CFX96 Deep Well, or CFX384 Optical Reaction ModuleNo

Instant incubation

Yes

Yes

Reaction Module

96-Well Fast

96–Deep Well

Dual 48/48-Well Fast

384-Well

Sample capacity

96 x 0.2 ml tubes or
1 x 96-well plate

96 x 0.2 ml tubes,
48 x 0.5 ml tubes,
or 1 x 96-well plate2 x 48 x 0.2 ml tubes or2 x 48-well plates

1 x 384-well plate

Maximum ramp rate

5°C/sec

2.5°C/sec

4°C/sec

2.5°C/sec

Average ramp rate

3.3°C/sec

2°C/sec

3°C/sec

2°C/sec

Temperature range

0–100°C

0–100°C

0 –100°C

0 –100°C

Temperature accuracy±0.2°C of programmed
target at 90°C

±0.2°C of programmed
target at 90°C

±0.2°C of programmed
target at 90°C

±0.2°C of programmed
target at 90°C

Temperature uniformity±0.4°C well-to-well within 10 secof arrival at 90°C

±0.4°C well-to-well within 10 secof arrival at 90°C

±0.4°C well-to-well within 10 secof arrival at 90°C

±0.4°C well-to-well within 10 secof arrival at 90°C

Thermal Gradient (available on all reaction modules)
Gradient accuracy±0.2°C of programmed temperature at end rows

Row uniformity±0.4°C well-to-well (within row) within 10 sec of arrival at target temperature

Gradient range

30–100°C

Temperature differential range

1–24°C

Optical Reaction Module

CFX96

CFX384

Excitation

6 filtered LEDs

5 filtered LEDs

Detection

6 filtered photodiodes

5 filtered photodiodes

Range of excitation/emission wavelengths

450–730 nm

450–690 nm

Sensitivity

Detects 1 copy of target sequence in human genomic DNA

Detects 1 copy of target sequence in human genomic DNA

Dynamic range

10 orders of magnitude

10 orders of magnitude

Scan time
All channels
Single channel fast scan

12 sec3 sec<20 sec
8 sec

Visit bio-rad.com/ThermalCyclersMore for more information.

BIO-RAD LABORATORIES, INC. | 10

Ordering Information
Catalog #Description

Catalog #184-1100	C1000 Touch Thermal Cycler Chassis, includes USB flash drive,
power cord; does not include reaction module
185-1148	
C1000 Touch Thermal Cycler with Dual 48/48 Fast Reaction
Module, includes C1000 Touch Thermal Cycler Chassis,
dual 48/48 fast reaction module, USB flash drive
185-1196	
C1000 Touch Thermal Cycler with 96-Well Fast Reaction
Module, includes C1000 Touch Thermal Cycler Chassis,
96-well fast reaction module, USB flash drive
185-1197	
C1000 Touch Thermal Cycler with 96–Deep Well Reaction
Module, includes C1000 Touch Thermal Cycler Chassis,
96–deep well reaction module, USB flash drive
185-1138
C1000 Touch Thermal Cycler with 384-Well Reaction Module,
includes C1000 Touch Thermal Cycler Chassis, 384-well reaction
module, USB flash drive184-2000	S1000 Thermal Cycler Chassis, includes power cord;
does not include reaction module
185-2148	
S1000 Thermal Cycler with Dual 48/48 Fast Reaction Module,
includes S1000 Thermal Cycler Chassis, dual 48/48 fast
reaction module
185-2196	
S1000 Thermal Cycler with 96-Well Fast Reaction Module,
includes S1000 Thermal Cycler Chassis, 96-well fast
reaction module
185-2197	
S1000 Thermal Cycler with 96–Deep Well Reaction Module,
includes S1000 Thermal Cycler Chassis, 96–deep wellreaction module
185-2138
S1000 Thermal Cycler with 384-Well Reaction Module,
includes S1000 Thermal Cycler Chassis, 384-well reaction module184-5096
CFX96 Optical Reaction Module, for use with C1000 Touch
Thermal Cycler Chassis, includes CFX Manager Software, license
for qbase+ Software, communication cable, reagents, consumables
185-5196
CFX96 Touch Real-Time PCR Detection System, includes
C1000 Touch Thermal Cycler Chassis, CFX96 Optical Reaction
Module, CFX Manager Software, license for qbase+ Software,communication cable, reagents, consumables
184-5384	
CFX384 Optical Reaction Module, for use with C1000 Touch
Thermal Cycler Chassis, includes CFX Manager Software, license
for qbase+ Software, communication cable, reagents, consumables
185-5484 	CFX384 Touch Real-Time PCR Detection System, includes
C1000 Touch Thermal Cycler Chassis, CFX384 Optical Reaction
Module, CFX Manager Software, license for qbase+ Software,communication cable, reagents, consumables
184-0148
Dual 48/48 Fast Reaction Module, independent dual 48-wellreaction module, fits C1000, C1000 Touch, and S1000 Thermal
Cyclers, gradient enabled
184-0196
9 6-Well Fast Reaction Module, fits C1000, C1000 Touch,and S1000 Thermal Cyclers, gradient enabled
184-0197
9 6–Deep Well Reaction Module, fits C1000, C1000 Touch,and S1000 Thermal Cyclers, gradient enabled
184-0138	
384-Well Reaction Module, fits C1000, C1000 Touch,and S1000 Thermal Cyclers, gradient enabled
184-5008	
CFX Manager Software, Chinese Edition, includes
3 user licenses, installation CD, 3 HASP HL keys
184-5028 	CFX Manager Software, Russian Edition, includes
3 user licenses, installation CD, 3 HASP HL keys
184-5001	
CFX Manager Software, Security Edition, includes
1 user license, installation CD, HASP HL key
184-5025	Precision Melt Analysis™ Software, includes 2 user
licenses, installation CD, 2 HASP HL keys, melt calibration kit
170-8840	iScript Reverse Transcription Supermix for RT-qPCR,
25 x 20 μl reactions, includes 100 μl 5x iScript RT Supermix and
iScript RT Supermix No-RT Control

Description

170-8842	iScript Advanced cDNA Synthesis Kit for RT-qPCR,
50 x 20 μl reactions, includes 200 μl 5x iScript Advanced Reaction
Mix, 50 μl iScript Advanced Reverse Transcriptase
172-5270 	SsoAdvanced™ Universal SYBR® Green Supermix,
2 ml (2 x 1 ml vials), 200 x 20 μl reactions, 2x qPCR mix,
contains Sso7d fusion polymerase, ROX Normalization Dyes
172-5280 	SsoAdvanced Universal Probes Supermix, 2 ml (2 x 1 ml vials),
200 x 20 μl reactions, 2x qPCR mix, contains Sso7d fusion
polymerase, ROX Normalization Dyes
172-5160 	SsoAdvanced PreAmp Supermix, 1.25 ml (1 x 1.25 ml vial),
50 x 50 μl reactions, 2x PreAmp Mix, contains dNTPs,
Sso7d fusion polymerase, salts, enhancers, stabilizers,
other proprietary components
172-5150 	iTaq™ Universal SYBR® Green One-Step Kit, 1 ml (1 x 1 ml vial),
100 x 20 μl reactions, includes 2x one-step SYBR® Green Reaction
Mix containing iTaq DNA Polymerase, buffers, and ROX Normalization
Dyes; iScript Reverse Transcriptase; nuclease-free water
172-5140 	iTaq Universal Probes One-Step Kit, 1 ml (1 x 1 ml vial),
100 x 20 μl reactions, includes 2x one-step reaction mix containing
iTaq DNA Polymerase, buffers, and ROX Normalization Dyes;
iScript Reverse Transcriptase; nuclease-free water
172-5310	
iProof™ HF Master Mix, 100 x 50 μl reactions, premixed PCR
reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable formost templates
HSP-3805
Hard-Shell 384-Well Skirted PCR Plates, clear shell, white well, 50
MLP-9601 	Multiplate™ High-Profile 96-Well Unskirted PCR Plates,clear, 25 plates
MSB-1001
Microseal® 'B' Adhesive Seals, optically clear, 100
TBC-0802 	8-Tube Strips and Domed Cap Strips (0.2 ml), clear,
20 bags of 12 x 8-tube strips and 12 x 8-cap strips(1,920 PCR tubes and 1,920 caps)
TWI-0201	
PCR Tubes with Domed Caps (0.2 ml), clear, 1,000

Visit bio-rad.com/web/ThermalCyclersMore
for more information.
Cy is a trademark of GE Healthcare group companies. EvaGreen is a trademark of Biotium, Inc.
Bio-Rad Laboratories, Inc. is licensed by Biotium, Inc. to sell reagents containing EvaGreen Dyefor use in real-time PCR, for research purposes only. FAM, HEX, ROX, SYBR, and Texas Red
are trademarks of Life Technologies Corporation. Bio-Rad Laboratories, Inc. is licensed by Life
Technologies Corporation to sell reagents containing SYBR Green I for use in real-time PCR, forresearch purposes only. HASP is a trademark of Aladdin Knowledge Systems, Ltd. Quasar is atrademark of Biosearch Technologies, Inc. Windows is a trademark of Microsoft Corporation.
Bio-Rad’s thermal cyclers and real-time thermal cyclers are covered by one or more of the following
U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 6,767,512
and 7,074,367.
Purchase of iTaq DNA Polymerase includes an immunity from suit under patents specified in the
product insert to use only the amount purchased for the purchaser’s own internal research. No
other patent rights are conveyed expressly, by implication, or by estoppel. Further information onpurchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems,
850 Lincoln Centre Drive, Foster City, California 94404, USA.
The use of iQ, SsoAdvanced, and SsoFast Supermixes is covered by one or more of the following
U.S. patents and corresponding patent claims outside the U.S.: 5,804,375; 5,994,056; and6,171,785. The purchase of these products includes a limited, non-transferable immunity fromsuit under the foregoing patent claims for using only this amount of product for the purchaser’s
own internal research. No right under any other patent claim and no right to perform commercial
services of any kind, including without limitation reporting the results of purchaser’s activities fora fee or other commercial consideration, are conveyed expressly, by implication, or by estoppel.
These products are for research use only. Diagnostic uses under Roche patents require a separate
license from Roche. Further information on purchasing licenses may be obtained from the Directorof Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Hard-Shell Plates are covered by one or more of the following U.S. patents or their foreigncounterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.

Bio-RadLaboratories, Inc.
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