Real-Time PCR

CFX Opus Deepwell
Real-Time PCR System

Large Volumes for the Modern Detection Laboratory
The CFX Opus Deepwell Real-Time PCR
System brings modern quantitative PCR (qPCR)
to your nucleic acid detection lab. Whether
you are pooling samples, running nonprecious
matrices, or requiring large volumes for otherreasons, the CFX Opus Deepwell System brings
your workflow all the features and connectivity
you need.	– E mail your completed run

As with our other CFX Opus Systems, you can
use CFX Maestro Software (for PC) locally oruse your BR.io account to set up a run andaccess your results anytime and anywhere
you have internet access.

SystemLicensed for real-time PCR

Yes

Sample capacity

96 wellsBenefits■ One of the industry’s largest qPCR reaction
volumes, up to 125 μl■■ eaction volumes ≤10 µl depending on
Rthe application
 lexible connectivity and data managementF
options for access and transfer of runs andexperiment protocols	– Operate in stand-alone mode throughflexible connectivity options of wirelessnetwork (WiFi), Ethernet, and USB– Access the BR.io cloud platform forexperiment setup and data management atany convenient location with internet access	– Enjoy continued support for and control ofup to 4 CFX instruments with CFX Maestro
Software for Windows 10 (64-bit), andSecurity Edition

Visit bio-rad.com/CFXOpus for more information.

	 – S ave protocols and completed runs in
a network folder– Set
 up unique user names, with optional
passwords, to easily organize and findyour protocols and experiments
Specifications

Sample size	1–125 µl (10–125 µl
recommended)
Dimensions (W x D x H)		33 x 56 x 36 cm(13 x 22 x 14 in.)

Weight22 kg (48 lb)

Touch-screen user interface	Adjustable, with angle ofrotation 12–55ºCommunications	USB 2.0 or above, Ethernet,
WiFi
Electrical approvals

IEC, CE

Operating system

Windows 10 IoT

Optical Detection System
Excitation

6 filtered LEDs

Detection

6 filtered photodiodes

Range of excitation/
emission wavelengths

450–730 nm

Scan time
All channels
Single channel

12 sec
3 sec

Dynamic range
10 orders of magnitude
Sensitivity	Detects 1 copy of target
sequence in humangenomic DNA
Multiplex analysis

Up to 5 targets per wellcontinues

CFX Opus Deepwell Real-Time PCR SystemSpecifications

Catalog #96-Well Reaction Block
Sample block type
Fixed 96-well sample block
Heating and cooling method Peltier
Lid heating
30–110ºC
Temperature range
4–100ºC
Maximum ramp rate
2.5ºC/sec
Average ramp rate
2ºC/sec
Thermal accuracy±0.2ºC
Thermal uniformity	±0.4ºC (max – min ≤ 0.8ºC), measured 10 sec after theblock reaches target temperature
Gradient
Operational range
30–100ºC
Programmable span
1–24ºC

1845025

CFX Maestro Data Management and Analysis Software
Operating system	Windows 10 (64-bit)
MemoryMinimum of 1 GB

Data analysis modes

PCR quantification with standard curve		

Melt curve analysis		Gene expression analysis by relative quantity (∆Cq)
or normalized expression (∆∆Cq)
		Multiple-file gene expression analysis for comparisonof an unlimited number of quantification cycle (Cq) values		

Allelic discrimination		Endpoint analysis		Data analysis options include bar charts, box andwhisker plots, dot plots, clustergrams, scatter plots,
and volcano plots				

Statistics include t-test and analysis ofvariance (ANOVA)

Data export	Save, copy, and print all graphs and spreadsheetsfrom right-click menu		

Export specified data in multiple formats		Copy and paste into a Microsoft Excel, Word,or PowerPoint file		Print directly or save as PDFs customizable reports
containing run settings, data graphs, and spreadsheets		

Export to RDML

Image export	Export images at any pixel size and at a resolution
up to 600 dpi		

Save images as .bmp, .jpg, or .png files

 recision Melt Analysis Software, includes 2 user licenses,
P
installation CD, 2 HASP HL keys, melt calibration kit
1845098	
CFX Qualification Plate, 96-well1814000
PX1 PCR Plate Sealer, includes heat sealing instrument1814030	PCR Plate Heat Seal, pkg of 100, optically clear seals for use
with the PX1 PCR Plate Sealer
MSB1001	Microseal™ 'B' PCR Plate Sealing Film, pkg of 100, optically clearseals for PCR plates
HSP9655	
Hard-Shell™ 96-Well PCR Plates, pkg of 50, low profile, thin wall,
skirted, white shell/white wells
HSP9955	
Hard-Shell 96-Well PCR Plates, pkg of 50, low profile, thin wall,
skirted, white shell/white wells, barcoded
1708840	iScript Reverse Transcription Supermix for RT-qPCR,
25 x 20 µl reactions1725037
iScript Advanced cDNA Synthesis Kit for RT-qPCR,
25 x 20 µl reactions
1725270
SsoAdvanced Universal SYBR® Green Supermix,2 ml (2 x 1 ml), 200 x 20 µl reactions
1725280	
SsoAdvanced Universal Probes Supermix, 2 ml (2 x 1 ml),
200 x 20 µl reactions
1725120
iTaq Universal SYBR® Green Supermix, 2 ml (2 x 1 ml),
200 x 20 μl reactions
1725130	iTaq Universal Probes Supermix, 2 ml (2 x 1 ml),
200 x 20 μl reactions
1725200	SsoFast EvaGreen® Supermix, 2 ml (2 x 1 ml), 200 x 20 μl reactions
1725210	
SsoFast EvaGreen® Supermix with Low ROX, 2 ml (2 x 1 ml),
200 x 20 μl reactions
12010176	
Reliance One-Step Multiplex RT-qPCR Supermix, 1 ml (1 x 1 ml),
200 x 20 μl reactions
1725150
iTaq Universal SYBR® Green One-Step Kit, 100 x 20 μl reactions
1725140
iTaq Universal Probes One-Step Kit, 100 x 20 μl reactions
1725848	iQ Multiplex Powermix, 50 x 50 µl reactions
1725095	SingleShot SYBR® Green One-Step Kit for Cell Lysis andRT-qPCR, 100 x 50 µl reactions
1725160
SsoAdvanced PreAmp Supermix, 1.25 ml (1 x 1.25 ml),
50 x 50 µl reactions
17005726
SEQuoia Complete Stranded RNA Library Prep Kit, 24 reactions
17005710
SEQuoia Complete Stranded RNA Library Prep Kit, 96 reactions
12011928	SEQuoia Dual Indexed Primers Set, 12 vials of unique dual
indexed primers, 96 reactions
12011930	SEQuoia Dual Indexed Primers Plate, 96-well plate of uniquedual indexed primers, 96 reactions

Search CFX qPCR Systems in the Apple App Store and Android
Play Store for an augmented reality tour. Contact your local sales
representative for an in-person demonstration.
Visit bio-rad.com/CFXOpus for more information.

Ordering Information
All software for Windows unless otherwise noted.
Catalog #Description

Description

12016658	CFX Opus Deepwell Real-Time PCR System, includes power
cord, USB cable, and Ethernet cable; does not include CFX
Maestro Software or WiFi adaptor
12013758	
CFX Maestro Software
12004128
CFX Maestro Software for Mac
12012832
CFX Maestro Software, Security Edition, 1 license
12013028
CFX Maestro Software, Security Edition, 5 licenses
12012833
CFX Maestro Software, Russian Edition, 1 license
12012834
CFX Maestro Software, Chinese Edition, 1 license

BIO-RAD, HARD-SHELL, and MICROSEAL are trademarks of Bio-Rad Laboratories, Inc.
in certain jurisdictions. All trademarks used herein are the property of their respectiveowner. © 2023 Bio-Rad Laboratories, Inc.
EvaGreen is a trademark of Biotium, Inc. Bio-Rad Laboratories, Inc. is licensed by Biotium, Inc.
to sell reagents containing EvaGreen Dye for use in real-time PCR, for research purposes only.
SYBR is a trademark of Thermo Fisher Scientific Inc. Bio-Rad Laboratories, Inc. islicensed by Thermo Fisher Scientific Inc. to sell reagents containing SYBR Green I for use
in real-time PCR, for research purposes only.

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