Food Testing

iQ-Design Assay
Aspergillus Identification Solution
Quick Guide

20 μl

Powermix

PP MixA. flavus BC■

20 μl

Powermix

PP MixA. fumigatus BCPowermix

PP MixA. niger BCPP Mix
A. terreus BProbemix■20 μlPowermix

20 μl

CAmplification
mix

Prepare the PCR mix for each Test Group: A. flavus, A. fumigatus, A. niger, andA. terreus (if applicable to local requirements) (see PCR Mix Calculation Guide on back)Use primer-probe vial (PP Mix) A. flavus for Test Group iQ-DA Aspergillus flavus,PP Mix A. fumigatus for Test Group iQ-DA Aspergillus fumigatus, PP Mix A. niger for
Test Group iQ-DA Aspergillus niger, and PP Mix A. terreus for Test GroupiQ-DA Aspergillus terreus■

Distribute 20 µl/well of each PCR mix in a microplate■

Add 5 µl each of positive and negative controls to designated wells

PCR mixfor eachtest group

5 µl

■

Transfer 5 µl/well of extracted DNA (from iQ-Check™ Aspergillus spp. DNA extraction
preparation) to the PCR microplate for each Test GroupDo not vortex before collecting the sample.
Check that there are no bubbles.

■

■

■

■

■

Seal the PCR microplate
Launch CFX Manager Industrial Diagnostic Edition (IDE) Software to get thePlate Manager window
Select iQ-DA Aspergillus from the list of tests on the left side of the screen
Select the required wells for the Test Group iQ-DA A. flavus (labeled asiQ-DA Aspergillus in the software)
Apply sample types to the wells by selecting Positive Control, Negative Control,or Unknown
A separate positive control and negative control must be set up for each Test Group.

■■Enter the test group name (flavus, fumigatus, niger, or terreus), and sample name

Select Add under the Test Group tab on the left-hand column of the window to repeat
for Test Group iQ-DA A. fumigatus (labeled as iQ-DA Aspergillus_1), Test GroupiQ-DA A. niger (labeled as iQ-DA Aspergillus_2), and Test Group iQ-DA A. terreus(labeled as iQ-DA Aspergillus_3)

For detailed instructions, review the kit user guide.

Visit bio-rad.com/FoodScience for more information.

iQ-Design Assay Aspergillus Identification Solution Quick Guide■■■

To duplicate the sample names for all Test Groups, select the Table View tabWith iQ-DA Aspergillus selected in the Test Group dropdown, select the Sample Namecolumn and then copy (Ctrl + C)
In the Test Group List dropdown, move to iQ-DA Aspergillus_1, then select the Sample
Name column and paste (Ctrl + V)■

Repeat for iQ-DA Aspergillus_2 and iQ-DA Aspergillus_3■

Start the amplification by clicking Start PCR Run

PCR Mix Calculation Guide for the iQ-Design Assays
To find the correct volumes to use when preparing the PCR mix, add the total number of samples and controls to be analyzed and find thecorresponding volumes of iQ-Design Assay Primer-Probe Mix and iQ Multiplex Powermix in the table.
Total Number ofSamples and Controls

iQ-Design Assay
Primer-Probe Mix, μl

iQ Multiplex Powermix
Amplification Mix, μl

Total Number ofSamples and Controls

iQ-Design Assay
Primer-Probe Mix, μl

iQ Multiplex Powermix
Amplification Mix, μl

17.5

12.5

17135

225

21830

18141

235

324

40

19150

250

433

55

20

159

265

539

65

21165

275

648

80

22

174

290

757

95

23180

300

86310524189

315

972120

25

198

390

1078

130

26

204

340

1187

145

272133551296160

28222

370

13102170

29228

380

14111

185

30237395

15117195

31246

410

16126

210

32252

420

Visit bio-rad.com/Cannabis for more information.
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