MEXT

National
BioResource
Project

Message from the Program Director

Bioresources—the strains, populations, tissues, cellsof bioresources and one center that provides informationand genes of animals, plants and microorganisms usedon these resources. The impor tance and value offor research and development, and the information

the bioresources established under this project areassociated with these materials—represent essential

continuously being elevated by the addition of genomic

infrastructure for life sciences. Bioresources areresources and development of preservation technologies.

developed over many years of painstaking work andIn fact, several of our bioresource centers have been

form the foundation of future research. Sharing themrecognized as meeting the highest global standards.

among researchers is vital for advancing research andCurrently, the Program Director (PD) and Program

development. In addition, providing a common set of

Officers (PO) are responsible for promoting the activities

bioresources for use within the scientific communityof the NBRP according to current trends in life sciences.

makes it possible to compare research results acrossstudies and will ultimately enhance reproducibility.

Finally, I would like to emphasize a lesson we learned

Developing outstanding collections of bioresources willfrom the Great East Japan Earthquake: bioresources

therefore give Japan an internationally competitive edge

cannot be restored once they are lost. Since the 2011

in life sciences.

disaster, we have developed a backup system that
includes long-term cryopreservation of bioresources.

In accordance with the Japanese Government’s Science

H o w e v e r, t h e e m e r g e n c e o f C OV I D -19 i n 2 0 2 0

and Technology Basic Plans, in FY2002, the Ministrynecessitated restrictions to staff attendance, which putof Education, Culture, Sports, Science and Technology

the maintenance and provision of bioresources at risk.

(MEXT) established the National BioResource Project

To solve this problem, we are promoting a labor-saving(NBRP) to construct a framework for the systematic

approach to bioresource maintenance and the adoption

collection, preservation, and distribution of bioresources,of remote monitoring systems. We will continue to strive

with a special focus on resources that require strategic

to maintain and provide bioresources even in the face ofdevelopment by the national government. The importancecrises such as the current pandemic. Your cooperationof bioresources continues to be stipulated in the Basicand support of this project is greatly appreciated.

Plans, with the current Sixth Plan (FY2021–FY2025)
outlining the need to strategically and systematicallyestablish and upgrade the bioresources and intellectual

April 2022

infrastructure necessary for data-driven research.
Yuji Kohara, Ph.D.

2

The NBRP is revised every five years. The fifth phase of

Program Director, NBRP

the NBRP, which started in FY2022, has so far seen the

Director, Database Center for Life Science,

addition of the “aged mice” and “mushrooms” categories.

Research Organization of Information and Systems,

At its core, the current NBRP consists of 33 categories

Inter-University Research Institute Corporation

Contents

Message from the Program Director .............................2
About the NBRP ...........................................................4
Placing an order and supporting the NBRP ..................5
History ..........................................................................6

ANIMALS
Mice .............................................................................7
Aged mice ....................................................................8
Rats .............................................................................9Japanese macaque ....................................................10
Chickens & Quails ......................................................11

MICROBES
Algae ..........................................................................27
Paramecium ...............................................................28
Cellular slime molds ....................................................29
Mushrooms ................................................................30
Yeast ..........................................................................31
General microbes .......................................................32
Prokaryotes (Escherichia coli, Bacillus subtilis) ............33
Pathogenic eukaryotic microbes .................................34
Pathogenic bacteria ....................................................35
Human pathogenic viruses .........................................36

Clawed frogs & Newts ................................................12
Zebrafish ....................................................................13
Medaka ......................................................................14
Ciona intestinalis .........................................................15
Drosophila ..................................................................16
Silkworms...................................................................17

CELLS, DNA MATERIALSHuman cord blood cells ..............................................37
Human and animal cells..............................................38
DNA-related materials.................................................39

Caenorhabditis elegans ..............................................18

INFORMATIONPLANTS
Arabidopsis ................................................................19
Rice ............................................................................20
Wheat.........................................................................21
Barley .........................................................................22
Lotus japonicus & Glycine max ...................................23
Tomatoes ...................................................................24
Chrysanthemums .......................................................25
Morning glories ...........................................................26

NBRP Information Center ...........................................40
GAIN / JBIF ................................................................41
ABS / Public relations .................................................42
Human resource development for promoting externalverification of animal experimentation..........................43
NBRP scheme ............................................................44
List of NBRP organizations .........................................45
NBRP network of Japan (5th Phase)...........................47

3

About the NBRP

Overview

1. Resource center programThis program aims to establish core centers that willcollect, preserve and provide bioresources that are

ME X T first established the NBRP in F Y2002 as aunique to Japan and form the foundation of life sciencecomprehensive approach to promote life sciences. The

research. The program consists of two subprograms:

project involves the systematic collection, preservation,

the first aims to improve the quality and value ofand distribution of bioresources—the strains, populations,the bioresources, and second aims to develop new

tissues, cells, and genetic materials of animals, plants,

technologies, such as those that will enable the long-term

and microbes and their associated information—that

preservation of bioresources.

require strategic development by the national government.

Value addition subprogram
Recipients of funds awarded under this subprogram

In the Sixth Science and Technology and Innovation

will analyze the genomic, metabolomic, proteomic,

Basic Plan (FY2021–FY2025), the government stipulated

metabolomic, and other characteristics of bioresources

the need to strategically and systematically provide

and biologically-derived components with beneficial

intellectual infrastructure and biological and genetic

physiological effects.

resources, such as genetic data, that form the foundation

Technology development subprogramof data-driven research. The NBRP therefore plans to

Recipients of funds awarded under this subprogram

build on its existing intellectual infrastructure by improving

will develop new technologies related to the collection,

the quality of available resources based on the diverse

proliferation, preser vation and provision (including

needs of researchers and promote the strategic collection

transportation) of bioresources. The goal of this programand utilization of these bioresources.

is to elevate the quality control, preservation technologies,
value and, in turn, quality of the resources.

Details

2. Information center programThis program aims to construct and make available

4

T h e NBRP i s mad e up of t wo p ro gra ms, (1) th e

databases containing such information as the location

resource center program and (2) the information centerand genetic and other characteristics of the bioresources

program, which together aim to facilitate the collection,collected at the core and sub-core centers. This program

preservation, and provision of bioresources and develop

will also raise the profile and visibility of the NBRP throughrelated technologies.

various public relations activities.

Placing an order and supporting the NBRP

Steps for requesting bioresourcesfrom NBRP
Anyone who intends to use our bioresources for his or her⨋

Order form⨋

Approval form (if the depositary has additional terms of use)⨋

Written acceptance to receive animals carrying recombinant
DNA (in the case of genetically modified organisms (GMOs)).

own research can purchase them by following the steps
below. We ask recipients to pay a fee that covers theFormal orders will be placed after the required documents,

actual cost of supplying the bioresources. Note that NBRP

such as the MTA, have been received and confirmed.

bioresources can be used for commercial purposes.
We are currently working to digitize the MTA agreement

1. Searching for a bioresource

process to shorten the ordering process.

Each bioresource has its own website. To access thewebsite of your desired bioresource, select the biological

3. Factors to consider for supply and provisionor genetic resource (bioresource) from the list on the

Organisms that have a long life-cycle will require more

“Resource Search” page of the NBRP website. Navigate

time before they can be sent to the recipient. Additionally,to the resource list or use the search function to find yourthe time required to provide the bioresource will vary

desired bioresource.

greatly depending on the strain or line’s proliferation
capacity and storage needs (e.g., factors for animals: live

2. Placing an order

or frozen (embryo or germ cell), season).

To place your order, use the web-order system (ifavailable), complete an order form (if available), or send

When requesting bioresources, we suggest that you

an inquiry by email.

contact the core or sub-core center supplying the

All orders must be accompanied by a completed Material

resource as soon as possible to agree on a realistic plan.

Transfer Agreement (MTA). In addition, the following
documents may also need to be submitted depending on

4. Final arrangements for sending bioresources

the regulations of the core or sub-core center supplying

When the bioresources are ready, we will contact you tothe resource:

coordinate the delivery date and make final arrangements

�. Searching fora bioresource

�. Placing an order�. Delivery

�. Supply and provision

�. Conﬁrmation of receiptof the bioresource

�. Final arrangements forsending bioresources

�. Payment5to confirm the delivery address and recipient.

2) send the details of the publication to the NBRP corecenter or sub-core center from which they purchased

5. Delivery / 6. Confirmation of receipt ofthe bioresource

the bioresource, upon publication.
Details of academic publications can also be registered

7. Payment

at the “Research Resource Circulation website”.

The payment method will dif fer depending on theinstitution (core or sub-core center) providing the

The NBRP collects research outcomes, including

resource and the delivery destination (Japan or overseas).

academic publications that make use of NBRP resources,

Payment can be made by bank transfer or credit card, orand adds this information to the NBRP resource

both. Please check the website of the facility from which

database. The generation of databases on the resources

you have or will make your purchase.

and related publications will further enhance the value ofour bioresources.

In addition to providing bioresources, the NBRP core andsub-core centers also make experiment and breeding

2. Deposit bioresources

protocols available on their website and organize

We welcome any requests to deposit bioresources totechnical training seminars. These resources and events

institutions funded under the “Resource Center Program”.

will be particularly informative and useful for first-timeOnce a deposit is made, the institution will reproduce,

users of NBRP bioresources.

send, and document the resources on your behalf.

Supporting the NBRP

D e p o s i to r s c a n s ti p u l ate va r i o u s c o n d i ti o n s fo r
third-party-use of the deposited bioresources. These
conditions can include citation of certain publications,

We ask the research community to support the NBRP in

restrictions on the purpose of use, and requirement for

the following two ways:

a separate license agreement for commercial use. If you
are considering making a deposit, please contact the

1. Submit details of academic publications

core center most relevant to your bioresource.

We ask that bioresource users
1) name the bioresource and its provider in the Materials

You can support the development of life science researchand Methods section or the Acknowledgements

in Japan by making your deposited bioresource(s)section of any academic publication that made use ofcontinuously available to the research community throughan NBRP resource, andthe NBRP. We thank you for your support.

History
1996
2002
2003

April
April

2007

April

2012

April

2015

2017

6

July

April

April

The Cabinet decides the first Science and Technology Basic Plan.

The National BioResource Project (NBRP) led by the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT),
as a part of the Research Revolution 2002 (RR2002) begins. The first phase of the NBRP starts off with 22 bioresource categories. The
NBRP is made up of the Resource Center Program and the Information Center Program.

Two bioresource categories are added to the Resource Center Program.

The second phase of the NBRP begins with 27 bioresource categories. The Value Addition Program and the Technology Development
Program begin.

The third phase of the NBRP begins with 29 bioresource categories.

The Japan Agency for Medical Research and Development (AMED) becomes responsible for the operation of the NBRP.

The fourth phase of the NBRP begins with 30 bioresource categories.

2021

March

One bioresource category is added to the Resource Center Program.

2022

April

The fifth phase of the NBRP begins with 33 bioresource categories.

April

MEXT becomes responsible for the operation of the NBRP for the second time.

The Value Addition Program and the Technology Development Program are reorganized as subprograms of the Resource Center Program.

Website: https://mus.brc.riken.jp/

Mice

ANIMALS

ANIMALS

Overview

the App gene that have been identified in AD patients. We
also provide the third generation AD model (RBRC11518),
which has mutations in both the App and Psen1 gene.

Mice are widely used as animal models in life sciencere s e a rc h to u n d e r s t a n d h i g h e r o rd e r b i o l o g i c a l

Wild-derived inbred strains
We also provide Japanese subspecies inbred strains

human he alth and conquer dise ase s. Numerous

such as MSM/MsRbrc (RBRC00209) and JF1/MsRbrc

genetically-uniform inbred mouse strains and their(RBRC00639) together with their genomic information.

genomic information are available for research purposes.

The enormous number of genomic polymorphisms

Additionally, gene-modified mice are being actively

present between these subspecies and common inbred

generated using gene-targeting in embryonic stem

strains is useful for understanding genomic function andcells and genome-editing in fer tilized eggs using

diverse biological phenotypes in humans.

PLANTS

phenomena, identif y approaches to promote

CRISPR/Cas9 technology.

Stock
The RIKEN BioResource Research Center (BRC) has
MICROBES

about 9,500 mouse strains predominantly gene-modified
strains (e.g., Tg, KO, cKO, KI mice), including:⨋

Human disease models: about 220 strains⨋

Cre/Flp driver mice: about 230 strains⨋

Fluorescent and luminescent reporter mice:about 1,000 strains

The second generation Alzheimer’s disease model C57BL/6-Apptm3(NL-G-F)Tcs(RBRC06344) recapitulates the amyloid pathology observed in patients.

Unique Features
CELLS, DNA MATERIALSRIKEN BRC has a collection of mouse strains developed
mainly in Japan that have been reported in academic
publications. The collection focuses on human diseasemodels and gene function analysis tools. We stock highly
unique mouse resources that cannot be obtained from
repositories overseas.

Update and Maintenance Activities
Core Center
RIKEN BRC will continue to add value to the collection by
providing related genomic and phenotypic information.

Alzheimer's disease (AD) model
The second generation AD model (RBRC06344) has

Contact
Core Center: E xperimental Animal Division,
RIKEN BioResource Research Center

been used around the world as a standard model

Principal Investigator: Atsushi Yoshiki

on which to test potential preventive and therapeutic

Email/URL: animal.brc@riken.jp

INFORMATION

Popular Strains and Applications

The Japanese wild-derived strain MSM/MsRbrc (RBRC00209) (left) andJapanese fancy strain JF1/MsRbrc (RBRC00639) (right).

treatments for AD. The model carries triple mutations in

7

Website: https://www.fbri-kobe.org/laboratory/animal_experimentation/nbrp/

ANIMALS

Aged mice

ANIMALS

Overview
It is often difficult for research institutions, including
universities, to rear mice for long periods and transfer

standardized aged mice and lay the foundations for aging
research in Japan.

Popular Strains and Applications

th e m b et we e n i nsti tu ti o ns. S i n c e e nv iro n m e nta l
PLANTS

conditions a nd long-te r m re ar ing c a n af fe ct the

C57BL/6 aged micephenotype of aged mice, breeding these mice in a

C 57B L /6 i s t h e s t a n d a r d m o u s e s t r a i n u s e d i n

uniform environment is crucial. The aged mice supplied

ex pe r im e nta l re se a rc h. M a ny pr io r studie s haveby the NBRP are bred for approximately two years

investigated various aspects of the aging process andin a uniform environment that is strictly controlled forage-related diseases using this mouse strain.

microbes. Our aged mice are therefore microbially safe
and can be used in aging research.

Stock
⨋

Standard mouse strains

MICROBES(C57BL/6J, C57BL/6N, BALB/cA): 3 strains⨋

Rearing conditions (single bred, group bred):
2 conditions

Unique Features
The aged mice supplied by the NBRP are housed for long
periods in a uniform environment that is strictly controlled
for microbiota. Apart from the National Institute on Aging,
CELLS, DNA MATERIALSINFORMATION

8Aged C57BL/6J mouse at 102 weeks.

which systematically supplies aged mice to researchers
in the United States, we are the only organization in
Japan that distributes aged mice.

Update and Maintenance Activities
Core Center
The Foundation for Biomedical Research and Innovation

Contact
Core Center: D epartment of Animal Experimentation,
Foundation for Biomedical Research andInnovation at Kobe

at Kobe started supplying aged mice as part of the

Principal Investigator: Shigeharu Wakana

NBRP in 2022. We hope to contribute to the wide use of

Email/URL: info-nbr-agedmouse@fbri.org

Website: http://www.anim.med.kyoto-u.ac.jp/NBR/default.aspx

Rats

ANIMALS

ANIMALS

Overview
Rats exhibit behaviors and metabolic systems that

Popular Strains and Applications
Severely immunodeficient rats
We have three immunodeficient strains: Il2rg KO (NBRP

facilitates experimental procedures. Rats are also

Rat No. 0883), Rag2 KO (NBRP Rat No. 0894) and Il2rg,

amenable to strict control of genetic and environmental

Rag2 double KO (NBRP Rat No. 0895). Human iPS cells

factors. Because of these superior properties, various

and cancer cells can be transplanted into these strains

rat disease models have been developed and used

for in vivo functional analysis.

PLANTS

resemble those of humans, and their moderate size

in medicine, pharmacology, life science, nutrition,neuroscience, genetics and other f ields. Recent

Cre driver rats

developments in genome editing technology have

We have a growing line-up of Cre driver rats which areenabled the development of complex genetically modified

predominantly neuronal lineages, including VGAT-cre

rats, further increasing their value as a bioresource.

(NBRP Rat No. 0839) and Drd2-cre (NBRP Rat No. 0843).
These Cre rats can be crossed with a Cre reporter rat (NBRP

Stock
Number of strains available: about 800 strains⨋

Information on 109 physiological characteristics:

Rat No. 0734) for visualization of specific cells and tissues.
MICROBES⨋

about 200 strains⨋

Genomic DNA and frozen sperm from ENU mutagenesis:
about 10,000 rats⨋

Recombinant inbred lines between LE/Stm and F344/Stm:
34 lines⨋

Genome sequencing information on F344/Stm lines
A rat expressing EGFP throughout its body. Many other reporter rats are available,
including those that express luciferase and cell-specific fluorescence markers.

Unique Features
The NBRP is home to the world's largest rat resource
center in terms of quality and quantity. We provide various
models of human disease such as hypertension, diabetes
and epilepsy that have been bred and developed inJapan. We also have severely immunodeficient rats

CELLS, DNA MATERIALS

and more.

produced by genome editing.

Update and Maintenance Activities

Rats that express photosensitive ion channels in a neuronal andCre-dependent manner can be used for optogenetic experiments.

Core Center
reproductive technologies and expand our Cre rat

Contact

collection.

Sub-Core Center
The University of Tokyo will add characterization data on

Core Center: Institute of Laboratory Animals, Graduate
School of Medicine, Kyoto University

Cre driver and reporter rats to enhance the value of ourPrincipal Investigator: Masahide Asano

high-quality rat resource.

Email/URL: h ttp://www.anim.med.kyoto-u.ac.jp/NBR/

INFORMATION

Kyoto University will develop and make available rat

contact.aspx

9Website: https://nihonzaru.jp/aboutus_2_e.html

ANIMALS

Japanese macaque

ANIMALS

Overview

such as rhesus and cynomolgus, Japanese macaques havea curious and calmer temperament, and highly-developed
cognitive and learning abilities, which make them suitable for

PLANTSThe Japanese macaque is a species of macaqueresearch into higher brain functions. They have contributedmonkey that is closely related to humans. It is often used

to a variety of basic research studies, including explorations

as an experimental animal model and is indispensableof the onset mechanism and pathology of neurologicalin various life science fields, including neurophysiology,

disorders such as dementia and Parkinson's disease, andinfectious disease/immunology, and regenerative

the development of treatments to restore function.

medicine. Japanese neuroscientists have produced
crucial research findings on higher brain functions using
Japanese macaques. Furthermore, Japanese macaques
exhibit specific susceptibility and immune response totropical and subtropical pathogens because they wereisolated from the Eurasian continent several hundredsof thousands of years ago. Such characteristics areadvantageous for comparative infectious disease studies.

MICROBES

Stock⨋Japanese macaque: 1 line

Unique Features

Japanese macaques are seasonal breeders that produce one offspring every
other year. It also takes time to raise them to the age needed for supply.

Japanese macaques are a species native to Japan. The
NBRP is the only organization that maintains a large
purpose-bred colony to provide macaques for research
CELLS, DNA MATERIALS

purposes. Global supply of macaques has become
unreliable due to the COVID-19 pandemic, which has
increased their value as a bioresource.

Update and Maintenance Activities
Core Center
Kyoto University’s Center for the Evolutionary Origins of
Human Behavior will conduct whole genome sequencingof breeding individuals to make available genetic
characteristics that differ across habitats.

Sub-Core Center

Group-reared in a semi-wild habitat, the monkeys we provide exhibit
well-developed motor skills and cognitive function.

The National Institute for Physiological Sciences will
INFORMATION

continue to work with Kyoto University’s Center for the
Evolutionary Origins of Human Behavior to improve theContact

efficiency and ensure smooth operation of the project.
Core Center: C enter for the Evolutionary Origins of Human

Popular Strains and Applications

Behavior, Kyoto University
Principal Investigator: Katsuki Nakamura
Email/URL: h t tps://w w w.nbr-macaque.pri.k yoto-u.

Compared to other Southeast-Asian macaque species,

10

ac.jp/?page_id=13

Website: https://www.agr.nagoya-u.ac.jp/~nbrp/en/index.html

ANIMALS

Chickens & Quails
ANIMALS

Overview
Among the approximately 9,600 bird species in existence,

respectively, are frequently used for live-imaging ofdevelopmental processes.

Quail WE strain, chicken M/O strain
These are widely used as standard strains for various

fields. For example, chickens are often used in embryology

purposes including chemical safety tests, physiology, andbecause their embryos are easy to manipulate for live-tracing

anatomy.

PLANTS

chickens and quails are most widely used in various researchof developmental processes. Although commercially available
chickens and quails with extremely high heterogeneity are
most commonly used in research today, use of the NBRP’s
genetically controlled strains, such as inbred strains, will
improve the reproducibility of experimental results.

Stock
⨋

Standard strains, mutant/disease model strains:
39 chicken strains, 23 quail strains
Genetically modified strains:
2 chicken strains, 1 quail strain (to be expanded)

The above strains can be supplied in the form of fertilized eggs,
blood, genomic DNA, live animals (chicks, adults) and more.
⨋

Primordial germ cell lines (including geneticallyTransgenic chickens and quails are suitable for live-imaging. Chickens andquails expressing EGFP and mCherry, respectively.

MICROBES⨋

manipulated lines): available from 2022

Unique Features
CELLS, DNA MATERIALS

Restrictions exist in the international transportation of live avian
resources to prevent epidemic expansion of influenza. The
NBRP provides strains that cannot be obtained elsewhere,
such as inbred strains and genetically-modified strains.

Update and Maintenance Activities
Core Center
Avian Bioscience Research Center at Nagoya University willsoon provide chicken primordial germ cell (PGC) lines, which
are suitable for genetic engineering including genome editing.

Sub-Core Center

Chicken resources available from the Avian Bioscience Research Center at
Nagoya University.

Hiroshima University is preparing to provide several

Popular Strains and Applications

Contact
Core Center: A vian Bioscience Research Center,
Graduate School of Bioagricultural Sciences,

Reporter-expressing transgenic lines (pLSi/ Δ
AeGFP-TG chicken, PGK:H2B-chFP-TG quail)

Chickens and quails that express EGFP and mCherry,

INFORMATION

Japanese chicken strains for research purposes.

Nagoya University
Principal Investigator: Ken-ichi Nishijima
Email/URL: abrc@agr.nagoya-u.ac.jp11Website: https://xenopus.nbrp.jp/NBRP_Xenopus/NBRP_Clawed_frogs_Newts_Top_EN.html

Clawed frogs & Newts

ANIMALS
ANIMALS

Overview

used in the first whole genome sequencing study. NH and NBH
are derived from the same colony from which Nigerian wasdeveloped, while IC is genetically distant from Nigerian-related

PLANTS

The short generation time of clawed frogs and newts makes

strains at the subspecies level. These strains are used forthem suitable for a variety of biological studies. Xenopus

developmental, genetic, evolutionary, and physiological studies.

tropicalis has a generation time of 4-6 months, making it

The X. tropicalis inbred strain (NBH) is often used in genome

suitable for genetic studies. Although Xenopus laevis hasediting research because of its robust eggs and embryos.

a slightly longer generation time (1-2 years), it is suitable fordevelopmental biology studies due to the ease with whichX. laevis inbred strain (J)embryos can be manipulated. Whole genome sequencing

This strain is widely used for developmental andinformation is available for both species. Finally, Pleurodeles

evolutionary studies.

waltl has a short generation time similar to that of Xenopus
tropicalis, and is especially suitable for regeneration studies.

P. waltl inbred strains (Y, T)
These strains are widely used for regeneration studies.

Stock
⨋

X. tropicalis (inbred, transgenic or mutant):

MICROBES

about 90 strains/lines⨋

X. laevis ("): about 10 strains/lines⨋

P. waltl ("): about 10 strains/lines⨋

Genomic DNA, cDNA clones, in situ hybridization kits,
CRISPR/Cas9-positive control kits: about 500 samples/clones/kits

Wild-type X. tropicalis (left: adult female, right: adult male).

Unique Features
CELLS, DNA MATERIALSThe highly inbred X. tropicalis, X. laevis, and P. waltl
strains were developed in Japan and are unique in theworld, making the NBRP home to a core international
stock center for these model amphibians.

Update and Maintenance Activities
Core Center
The Amphibian Research Center at Hiroshima University
will begin providing X. laevi s and P. waltl from 2022.
These species are often used together with X. tropicalis.

Sub-Core Centers
Yamagata University and Waseda University will continue

Top: Wild-type X. laevis (left: adult female, right: adult male).
Bottom: Wild-type P. waltl.

INFORMATION

to maintain backup storage of seed parents and frozen
sperm of valuable strains.

Contact

Popular Strains and Applications

Core Center: A
 mphibian Research Center, Hiroshima

X. tropicalis inbred strains (NA, NH, NBH, IC)

Principal Investigator: Hajime Ogino

University
NA is a direct descendant of Nigerian, the classic inbred strain

12

Email/URL: nbrpfrog@hiroshima-u.ac.jp

Website: https://shigen.nig.ac.jp/zebra/index_en.html

Zebrafish

ANIMALS

ANIMALS

Overview

pathway, a conserved neural circuit among vertebrates
that encodes the expected level of aversiveness forlearning the appropriate behavior to avoid danger.

Zebrafish are ver tebrates that remain transparent
throughout embr yogenesis. They are also easy to

Fucci fish (Depositor: Dr. Atsushi Miyawaki,

breed, have a short life cycle, and are amenable to

RIKEN)In Fucci fish, the nuclei of cells in S/G2/M phasecharacteristics, zebrafish are used to study biologicalfluoresce green while the nuclei of cells in G1 phasere g u l ato r y p ro c e s s e s s u c h a s th o s e i nvo l ve d i nfluoresce orange using the Fucci method invented by Dr.

development and regeneration using molecular genetics

Atsushi Miyawaki. The phase of cell division can thus be

and imaging technology. Use of zebrafish as a substituteobserved from the fluorescent color.

PLANTS

mutation and genetic modification. As a result of thesefor mammalian models has increased in recent years,
reflecting the rise in awareness of animal welfare issues.

Stock
Wild-type: 1 strain⨋

Mutant lines: about 400 strains⨋

Transgenic lines: about 1,800 strains⨋

TILLING: 4,000 strains

MICROBES⨋

Unique Features
By using the efficient sperm cryopreservation method
developed under the NBRP, we are able to supply strainsthat were developed uniquely in Japan to researchers in

CELLS, DNA MATERIALSJapan and around the world.

Adult zebrafish, one cell-stage embryo, and 16-hour post-fertilizationembryo.

Update and Maintenance Activities
Core Center
RIKEN Center for Brain Science will continue to collect,preserve, and distribute zebrafish strains produced inJapan.

Sub-Core Centers
The National Institute of Genetics and National Institutefor Basic Biology will continue to preserve and distributegene and enhancer trap lines and nervous systemcell-manipulated lines.

Contact

dao:cre-mCherry; vglut2a:loxP-DsRed-loxP-GFP

Core Center: R IKEN Center for Brain Science(RIKEN)

Principal Investigator: Hitoshi Okamoto

This strain has been used to study the habebulo-raphe

INFORMATION

Popular Strains and Applications

Left: The brain of a transgenic fish in which the output pathway from thehabenula is fluorescently labeled. Right: Fucci fish embryo.

Email/URL: hitoshi.okamoto@riken.jp

13Website: https://shigen.nig.ac.jp/medaka/

MedakaANIMALS
ANIMALS

Overview
Medaka is a small freshwater fish with a relatively short

Popular Strains and Applications
HdrR-II1 inbred line

PLANTS

life cycle (3 months) and small genome size (800 Mbp).

This inbred line is derived from d-rR/Nagoya, which was

Medaka can adapt to low temperatures (4℃) in theestablished by Dr. Toki-o Yamamoto. The HdrR-II1 line

winter and high temperatures (40℃) in the summer, as

was used to determine the sequence of the medaka

well as to relatively high salinity environments. In addition

genome, and genome annotation was subsequently

to medaka, the NBRP also provides medaka-related

performed based on the HdrR-II1 genome sequence.

species and hatching enzymes essential for embryonic

Genome sequences of other inbred lines, including

manipulation, along with high quality genome sequence

HNI-II, which is derived from wild medaka in Niigata, andinformation and genome annotations.

HSOK, which is derived from medaka in Sokcho, Korea,
are also available. HNI-II is a sister clade to HdrRII-1, and

Stock
⨋

HSOK can be used as an outgroup.

Standard strains and wild-derived strains from Japan,
China, and Korea: about 100 strains

OK-Cab strain

MICROBES⨋

Mutant and transgenic lines: about 730 strains

OK-cab is a strain derived from medaka provided by⨋Related species: about 10 strains

Carolina Biological Supply in the USA. It can be bred in⨋

Genome resources (cDNA, BAC, Fosmid clones):

many laboratories around the world because of its highabout 730,000 clones

fecundity and ease of breeding.

⨋

Hatching enzymes

Unique Features
The NBRP is home to the only Medaka biological
CELLS, DNA MATERIALS

and genetic resource center in the world. We provide
biological resources, genome resources, hatching
enzymes, and various related databases.

Update and Maintenance Activities
Core Center
In collaboration with Tohoku University, the National
Institute of Basic Biology and Utsunomiya University,
will sequence the genomes of 100 wild-derived strains
and construct a more accurate reference genome using
PacBio Hi-Fi reads.

The National Institute for Basic Biology and Utsunomiya University
offer a variety of resources including standard strains, inbred strains,
medaka-related species, and transgenic strains.

Sub-Core Center
INFORMATIONThe National Institute of Genetics and other facilities
will develop user-friendly web tools to integrate medaka

Contact

genome information and omics data.
Core Center: L ab of Bioresources, National Institute for
Basic Biology
Principal Investigator: Kiyoshi Naruse
Email/URL: mbrc@nibb.ac.jp

14Website: https://marinebio.nbrp.jp/ciona/

ANIMALS

Ciona intestinalis
ANIMALS

Overview

produce various responses to stimuli in its environment.
Together with the connectome of the larval nervous system,transgenic lines that mark specific neuronal populations
are a useful tool in neurobiology. We have collected various

chordate, ascidian. Ascidian is the closest living relative

neuronal marker lines, which have been used to characterizeof vertebrates. Elucidating the molecular mechanisms of

the specific mechanism of terminal differentiation of

ascidians is necessary for understanding the evolution ofneurons, to identify the neurons that respond to stimuli, andchordates. The simple body structures and genome of

to trace cells during metamorphosis and regeneration.

Ciona make it well-suited for molecular studies. Its genome
sequence has been well-annotated and gene resources

PLANTS

Ciona intestinalis belongs to the marine invertebrate

Wild-typecomprehensively prepared. It has a generation time of only 3

We c u l t i va te w i l d -t y p e C i o n a a s c l o s e d c o l o ny

months and inland culture systems have been established.

populations so that we can provide this resource
throughout most of the year. Although Ciona has frequent

Stockgenetic variations in its genome, closed colonies can limit⨋

Mutants and transgenic lines: about 150 lines

these variations. Our sequenced genomes of wild-type⨋

Wild-type (closed colonies established from wild

populations are useful for identifying genetic variations.
MICROBES

populations): 2 populations⨋

Expression vectors: about 400 clones

Unique Features
The NBRP cultivates wild-type populations to ensurethat they are available throughout most of the year. Ourcollection of transgenic lines covers nearly the entire setof tissue/organ visual markers. We are the only center

Update and Maintenance Activities

Larva of transgenic lines expressing the Kaede reporter in acetylcholine-positive(top) and glutamate-positive (bottom) neurons.

Core Center
The University of Tsukuba’s Shimoda Marine ResearchCenter is working to improve the quality of our transgenic
collections.

Sub-Core Centers

CELLS, DNA MATERIALS

that strategically provides live Ciona material.

Kyoto University (Graduate School of Science and Maizuru
Fisheries Research Station) and the University of Tokyo(Misaki Marine Biological Station) will continue to cultivate
wild-type populations and conduct genomic analyses of

Wild-type Ciona intestinalis cultivated in the ocean.

Popular Strains and Applications

Contact
Core Center: S himoda Marine Research Center,

Neuron marker transgenic lines(such as Tg[MiCiVACHTK]5)
Despite having a limited number of neurons, Ciona can

INFORMATION

wild-types to provide high quality material to researchers.

University of Tsukuba
Principal Investigator: Yasunori Sasakura
Email/URL: sasakura@shimoda.tsukuba.ac.jp15Website: https://fruitfly.jp/flystock/index_e.html

ANIMALS

Drosophila

ANIMALS

Overview

researchers can efficiently produce gene knockout
flies. These gene knockout flies can then be used forscreening purposes, which will help advance basic

Drosophila is a small insect that is widely used as a

biological research.

model organism in both basic biological research andmedical science because it shares 70% or more of its

NP strains

PLANTS

genes with humans. It has a short generation time ofThis is a collection of GAL4 enhancer trap insertion

only 2 weeks and is easy to cultivate. Moreover, thestrains produced by the NP consortium in Japan that has

Drosophila genome has been sequenced and precisely

been widely used both domestically and internationallyannotated. The last 20 years has seen significant growth

for tissue-specific expression and knockdown using theand advancement of Drosophila resources, and the pace

GAL4/UAS system.

of advancement is continuing to increase in Japan.

Stock

MICROBES⨋

RNAi strains: about 14,000 strains⨋

FlyCas9 strains: about 30 strains⨋

Basic strains: about 3,700 strains⨋

NP strains: about 4,200 strains⨋

Regional wild/mutant strains: about 1,500 strains

Unique Features
The NBRP has developed unique strains such as FlyCas9
strains. We are the only comprehensive Drosophila

Drosophila.

resource consortium in Asia and have the largest scale of
CELLS, DNA MATERIALS

Drosophila species.
Update and Maintenance Activities
Core Center
T he National Institute of Genetics is work ing onrevamping our catalog database to improve international
visibility and user experience. We provide systematic
genome-edited strains that are unique around the world.

Sub-Core Centers
Kyoto Institute of Technology will continue to provide
basic strains and human ORF strains, and cryopreserve

EGFP-expressing strain driven by a tissue-specific promoter (left: adult,
right: pupa).

primordial germ cells. Kyorin University will continue to
INFORMATION

act as a global Drosophila species stock center.

Popular Strains and Applications

Core Center: D epartment of Chromosome Science,
National Institute of Genetics

FlyCas9 strains (CAS-0001–CAS-0004）

By crossing this strain with a guide RNA strain,

16

Contact

Principal Investigator: Kuniaki Saito
Email/URL: saitok@nig.ac.jp, flyadmin@nig.ac.jp

Website: https://silkworm.nbrp.jp/index_en.html

ANIMALS

Silkworms
ANIMALS

Overview

silkworm genome and is widely used because of its resistanceto disease. This consomic line was developed by replacing
each chromosome of the ancestral silkworm species, Bombyx

Silkworms are easy to rear, have a short generation time

mori, with that of the p50 lines. We expect the number ofof approximately 50 days and are amenable to genome

requests for this consomic line to increase because it allows

editing. Females can lay 500 eggs in one night, all ofresearchers to explore the domestication process of silkworms.

world's most diverse selection of silkworm mutations.

Silkworm strains (NB1, NB2, NB3)

We have also developed cryopreservation techniques

These three strains are easy to raise and suitable forfor ovaries and sperm to facilitate the preservation of

beginners. NB2 can be easily identified as male or female

genome-edited lines.

by its appearance in the larval stage. NB3 is large and can

PLANTS

which are genetically uniform. The NBRP is home to the

be inoculated with drugs and pathogens by injection, and is

Stock
⨋

widely used in the fields of medicine and pharmaceuticals.

Genetic mutation lines (mostly spontaneous mutations):
about 500 lines⨋

Consomic and congenic lines with wild-type arachnids:
Genome-engineered lines: about 150 lines⨋

Wild silkworm species: 11 species⨋

Cultured cell lines: 3 strains (to be expanded)

MICROBES

about 200 lines⨋

Unique Features
T h e NB R P i s h o m e to th e wo r l d's m o st d i ve r s esilkworm mutants. The majority were generated throughDiverse silkworm cocoons. The variation in cocoon shape is due todifferences in larval cocooning behavior, and is useful for behavioral analysis.

CELLS, DNA MATERIALS

spontaneous mutations and allow us to explore thediversity of life and the mysteries of nature and chance.

Update and Maintenance Activities
Core Center
Ky us hu Uni ve r si t y will c o ntinu e to add g e nomi cinformation on frequently-requested representative strains
and enhance the maintenance of cultured cells to elevatethe value of our resource.

Sub-Core Cetner
Shinshu University will develop an efficient and reliable

Silkworm larval mutants. There was a time when 40 billion silkworm
larvae were reared annually in Japan, which led to the discovery of uniquespontaneous mutations.

distribution system for wild silkworm resources which are

Popular Strains and Applications
Silkworm p50 line (a consomic line between
silkworm and silkworm)
The p50 strain is the standard strain used to analyze theContact
Core Center: Institute of Genetic Resources,
Graduate School of Bioresources and

INFORMATION

closely related to silkworms.

Bioenvironmental Science, Kyushu University
Principal Investigator: Tsuguru Fujii
Email/URL: t.fujii@agr.kyushu-u.ac.jp17Website: https://shigen.nig.ac.jp/c.elegans/

ANIMALS

Caenorhabditis elegans

ANIMALS

Overview

the function of protein kinase LRK-1 in C. elegans
may facilitate the development of new treatments for
Parkinson’s disease.

C. elegans is a useful animal model for human diseases
because around 40% of its genes are orthologous to thoseof humans. It has a short life cycle of about 3 days at 20˚C
PLANTSand is amenable to culture with E. coli. Further, C. elegans
has only about 1,000 somatic cells, the cell lineages ofwhich have been comprehensively described. Thanks toserial electron microscopic images, detailed descriptions of
its morphology have also been produced, and it was the firstmulticellular organism to have its whole genome sequenced.
Thus, C. elegans is suitable for genetic analyses, and its
mutants are useful for exploring gene function.

Stock
MICROBES⨋

Deletion mutant lines: about 13,100 lines⨋

High function balancers: 69 lines⨋

Cre transgenic lines: 43 lines

C. elegans balancer tmC6[dpy-2(tmIs1189)]. Pharyngeal fluorescence isused as an indicator that the balancer is present.

Unique Features
The NBRP has about half the number of deletion mutants
as there are genes in wild-type C. elegans. We also havehigh function balancers that do not exhibit aneuploidy.
CELLS, DNA MATERIALS

Update and Maintenance Activities
Core CenterTokyo Women’s Medical University will continue to collectand distribute deletion mutants, high function balancers,
and Cre-transgenic C. elegans strains.

Popular Strains and Applications

Deletion mutant egl-26(tm1244). This strain shows the egg-laying defective(egl) phenotype. The mutant has eggs and L1 larvae in its uterus.

Contact

lrk-1 (tm1898) deletion mutant
Because the C. elegans lrk-1 gene is homologous to a
INFORMATION

18

familial Parkinson’s disease gene in humans, a mutant

Core Center: D epartment of Physiology, Tokyo Women’s
Medical University School of Medicine

lacking the lrk-1 gene (tm1898) is an important animal

Principal Investigator: Shohei Mitani

model for understanding the disease. Further, elucidating

Email/URL: mitani.shohei@twmu.ac.jp

Website: https://epd.brc.riken.jp

PLANTS

Arabidopsis
ANIMALS

Overview

100 representative lines from our collection to establish
a set of natural accessions which we will provide as a
new resource named psw00001. Each line in this set
was crossed with the standard lines Col and Ler, andthe progeny seeds of the F2 generation are available fora short life cycle of only 3 months. Under laboratory

quantitative trait locus analysis. Furthermore, a set of seed

conditions, each Arabidopsis plant produces thousands

pools is available for screening purposes. Together with theof seeds. In addition, it requires a simple procedure foravailability of genotyping services, we expect that thesetransformation, and the plant’s entire genome sequence

resources will accelerate the study of quantitative traits.

PLANTS

Arabidopsis is a recognized representative model plantused in various research fields. It is a small plant withhas been annotated. Japan is a leader in the field of
cultured plant cells, which have been widely used in studies
on cell biology and the production of useful substances.

Stock
Mutants and transformed lines: about 350 lines⨋

Ecotypes: about 530 lines⨋

Gene-knockout lines: about 20,000 lines⨋

Arabidopsis cDNA clones: about 240,000 lines⨋

Cultured plant cell lines: about 70 lines

MICROBES⨋

Unique Features
The RIKEN BioResource Research Center (BRC) is
home to a unique resource center in Asia that distributes

Japanese accessions.

Arabidopsis-related materials developed in Japan. In
CELLS, DNA MATERIALS

addition, we are the only institution in the world that
distributes plant-cultured cells to the international
research community.

Update and Maintenance Activities
Core Center
The E xperimental Plant Division at RIKEN BRC ischaracterizing the phenotypes of Japanese accessions
using the RIKEN Integrated Plant Phenotyping System(RIPPS). The data will be made available together with the
genome sequences.
Sampling locations of Japanese accessions.

Contact

Natural accessions that grow under various environmental
conditions harbor polymorphisms in both genotype andphenotype. We distribute natural accessions collected fromCore Center: E xperimental Plant Division,

INFORMATION

Popular Strains and Applications

RIKEN BioResource Research Center

various regions around the world. In addition, based on

Principal Investigator: Masatomo Kobayashi

single-nucleotide polymorphism data, we have selected

Email/URL: plant.brc@riken.jp

19Website: https://shigen.nig.ac.jp/rice/oryzabase/

PLANTSRice

ANIMALS

Overview

are capable of transformation. Wild Oryza accessions are
suitable for studies aimed at identifying valuable genes
for use in breeding and studies on genome evolution

Rice is an essential and major calorie source required tousing species that have adapted to diverse environments.

feed the large population on Earth, and rice plants have

Closely related wild species such as O. rufipogon areco-evolved with humanity. Oryza genus-related genetic

also used to study the rice domestication process.

PLANTS

resources, including wild progenitor species, are usedin various fields of research, including breeding science

MNU-induced mutation lines

and basic research in genetics and biology. We provide

We also offer mutation lines derived from rice cultivars

wild Oryza accessions and mutation lines derived from(e.g., Kinmaze, Taichung 65, Kitaake, Yukihikari). These

cultivated species.

mutation lines were produced by treating fertilized eggs
with MNU. These lines are used in basic research suchStockas morphological and physiological studies of rice, and⨋Wild Oryza accessions: about 1,700 accessions

research on agronomically important traits such as⨋

MNU-induced mutation lines

disease resistance and grain quality.

(derived from Kinmaze, TC65, Kitaake & Yukihikari)
MICROBES⨋

Chromosome segment substitution lines (CSSLs) and others(chromosomes of Oryza glaberrima, O. meridionalis, O.

glumaepatula, O. sativa indica and O. sativa japonica
are introduced into cultivated rice and more.)

Unique FeaturesNBRP Rice is home to one of few facilities to preserveand provide an eclectic collection of wild Or y za
CELLS, DNA MATERIALS

accessions in the world. Wild Oryza species are valuablegenetic resources as their habitats are in decline due tourban development and environmental changes.

Diverse panicle shapes of wild Oryza accessions. The size of the panicle
and the length and number of branches differ among accessions.

Update and Maintenance Activities
Core Center
The National Institute of Genetics will continue to provide
wild Or yza accessions and their genetic/genomic
information through Oryzabase.

Sub-Core Center
Kyushu University will continue to provide experimental
lines, such as mutation lines of cultivars and CSSLs of
INFORMATION

wild Oryza species.

Popular Strains and Applications

Contact
Core Center: D epartment of Genomics and Evolutionary

Wild Oryza accessions

20

Variation in leaf morphology among MNU-induced mutation lines.

Biology, National Institute of Genetics

We offer about 1,700 accessions of wild Oryza species

Principal Investigator: Yutaka Satocovering 21 species in the Oryza genus, some of which

Email/URL: yusato@nig.ac.jp

Website: https://shigen.nig.ac.jp/wheat/komugi/

PLANTSWheat
ANIMALS

Overview

is a high likelihood of discovering unknown alleles among
Asian varieties. This line is a representative Asian wheat
variety, and its genomic sequence has been determined5).

Wheat, one of the world's three major cereals, has a

It has been used for gene isolation of agronomic traits. We

complex genome structure that can be diploid (2n = 14;

have developed experimental lines derived from crosses

Einkorn wheat), tetraploid (2n = 28; Emmer wheat), or

between this line and 24 Asian wheat lines.

a d v a n c e s h ave m a d e w h o l e g e n o m e s e q u e n c eʻFielderʼ (LPGKU2330)

information and transformation available, opening new

While wheat is often difficult to transform, this line is one

doors for wheat science research1), 2), 3). Wheat researchof the standard varieties used for transformation. Givencan contribute to everything from basic biological sciencethat its genome sequence has already been determined,

to global food issues.

reverse genetics is possible using this line.

PLANTS

hexaploid (2n = 42; common wheat). Recent scientific

Stock
Landraces/Cultivars: about 10,800 lines⨋

Wild species: about 4,792 strains⨋

Experimental lines: about 1,575 strains⨋

NAM population of common wheat: 4,892 lines

MICROBES⨋

Unique Features
While most genebank collections around the world focus
on cultivars, the NBRP provides an extensive number oflandraces, wild relatives, and experimental lines.
ND-FISH of ‘Norin 61’ the representative bread wheat variety in Asia.

CELLS, DNA MATERIALS

Update and Maintenance Activities
Core Center
Kyoto University is developing large-scale recombinant
inbred lines in the two types of wheat that have playeda significant role in the polyploidy evolution of wheat:tetraploid wheat and the diploid wild relative, Ae. tauschii.

Popular Strains and Applications
ʻNorin 61ʼ (LPGKU2305)

Wheat in the West is genetically highly differentiated fromA field photo of a NAM population of common wheat, derived using breadwheat ‘Norin 61’ as the fixed parent.

been under-utilized in the world of wheat breeding5), there
1) International Wheat Genome Sequencing Consortium (IWGSC),
Science 361: eaar7191, 2018
2) Abe et al. 20193) Sato et al. 2021
4) Balfourier et al. 20195) Walkowiak et al. 2020

Contact
Core Center: G raduate School of Agriculture,

INFORMATION

that in Asia4). As Asian genetic resources have generally

Kyoto University
Principal Investigator: Ryohei Terauchi
Email/URL: nbrpkmg.ku@gmail.com

21

Website: http://earth.nig.ac.jp/~dclust/cgi-bin/index.cgi?lang=en

BarleyPLANTSANIMALS

Overview

Barley Standard Varieties (274 lines)
These varieties are a core collection selected from
NBRP strains with reliable collection-site information.

PLANTS

Barley is used for brewing, food, and feed. It exists

Single-nucleotide polymorphism information on theseas a variety of wild and cultivated species with highvarieties is available from our online database. This

adaptability and resistance to environmental changes

collection has been used to represent the world barleysuch as drought, salt, and moisture damage. Due to its

diversity, particularly in molecular evolution studies of

diploid genome structure, mutations are easy to detect

genes and genome-wide association studies.

in cultivated species. A high-quality genome assembly
has also been completed. Therefore, barley is used asa model in plant science and practical applications,
including the identification of useful genes.

Stock
⨋

Cultivated and wild strains collected from around the world:
about 11,000 lines

MICROBES⨋

Genetic experimental stocks: about 9,000 lines⨋

Genome-modified lines: 3 lines⨋

Full-length cDNA clones (to be expanded in 2022):
about 220,000 clones⨋

Genomic DNA: about 3,300 samples

Barley seeds.

Unique Features
The NBRP is one of the world's five best barley resource
CELLS, DNA MATERIALS

centers located in East Asia. We preserve barley strainsthat have been independently collected and developed,
and provide the genome assemblies of key strains.

Update and Maintenance Activities
Core Center
Okayama University is working to provide high-quality
genome DNA, trait information and genome information
on barley strains.

Popular Strains and Applications
INFORMATION

Contact

Haruna Nijo (J247)
Haruna Nijo is a key Japanese strain that has beenused to develop a BAC library, full-length cDNAs and aCore Center: Institute of Plant Science and Resources,
Okayama University

molecular genetic map. The chromosome-scale genome

Principal Investigator: Kazuhiro Satoassembly of this strain has been released and used for

Email/URL: barley@okayama-u.ac.jp

gene isolation and genetic analysis of useful traits.

22

Diverse barley spike patterns.

Website: https://legumebase.nbrp.jp/

Lotus japonicus & Glycine max

PLANTSANIMALS

Overview

symbiosis-related genes. This was a globally acclaimed
achievement because there are only a small fraction ofpotentially discoverable genes left; many genes related

Lotus japonicus is a wild legume that grows naturally into symbiosis between plants and microorganisms haveJapan and is widely used as a model legume in basic

already been isolated (Nat Commun 9:499 2018, PLoS

research. This is because the plant is easy to grow and

Genet 15(1): e1007966 2019).

the sequence of which has been determined and madeWild L. japonicus line (MG line)

publicly available. Glycine max is widely used around the

Genome analysis of 136 MG lines collected throughout

world as food, feed, and a high-value cash crop, because

Japan and cultivation experiments on these strains in

its seeds contain many functional components such asa wide range of environments have revealed that L.

isoflavones and saponins, in addition to proteins and lipids.

PLANTS

has a short life cycle (about 3 months) and small genome,

japonicus was first established in Kyushu approximately
20,0 0 0 y e a r s a g o a n d s u b s e q u e n t l y ex p a n d e dthroughout Japan. Fur thermore, genes related toExperimental lines of L. japonicus: 3 lines

overwintering and flowering regulation were found to be⨋

Japanese wild accessions: about 1,000 lines

crucial for adaptation of L. japonicus to northern Japan⨋

Recombinant inbred lines and taglines:(Nat Commun 11:253 2020).

about 1,600 lines⨋

DNA clones (including Rhizobium):⨋

Rhizobium STM mutant strains: about 7,000 lines

about 240,000 clones

MICROBES

Stock⨋

Unique Features
The NBRP provides a unique collection of wild strains
are the only organization that provides these resourcesin Asia and the only distributor of wild strains andWild Lotus japonicus growing near the mouths of rivers in Miyazaki city. It
grows in open areas, including riverbanks.

Rhizobium STM mutant strains in the world.
Update and Maintenance Activities
Core Center
University of Miyazaki will collect L. japonicus LORE1

CELLS, DNA MATERIALS

and Rhizobium for both L. japonicus and G. max. We

taglines, Rhizobium STM mutants, and develop a corecollection for G. soja.

Sub-Core Center
Tohoku University will select LORE1 core lines andVarious soybean seeds. More than 5,000 varieties of soybeans and wild
soybean (Glycine soja) grow naturally in Japan.

Rhizobium STM mutants.

Contact

Popular Strains and Applications

Core Center: F aculty of Regional Innovation,

Miyakojima MG-20 and Gifu B-129

Principal Investigator: Masatsugu Hashiguchi

INFORMATION

provide information on mutation inser tion sites in

University of Miyazaki
T h e s e l i n e s w e r e r e c e n t l y u s e d to i s o l a te n e w

Email/URL: legume@brc.miyazaki-u.ac.jp23Website (left: TOMATOMA) : https://tomatoma.nbrp.jp/(right: TOMATOMICS) : http://bioinf.mind.meiji.ac.jp/tomatomics/

PLANTS

Tomatoes

ANIMALS

Overview

phenotypes, including small plant size, short life cycle, anda complete life cycle in indoor growth rooms, making themsuitable experimental model plants compared to standard

PLANTS

Studies on gene function, cultivation characteristics, andtomato cultivars, which require cultivation in greenhouses.

fruit chemistry of tomatoes attract much interest fromMicro-Tom is used for various types of research including

scientists because tomatoes have one of the largest

fruit development, metabolic regulation, hormone

cultivation areas among all fruits and vegetables. In

response, and stress responses.

addition, tomatoes have the potential to become thenext model plant for both basic and applied research.

Micro-Tom mutants (TOMJPE/M/W series)

Tomato research contributes to the understanding of fruit

Because this collection is one of the largest mutant tomato

development and light-independent flowering, both ofresources in the world, we receive many international requests

which are unexplorable physiological events in traditional

to use phenotypic mutants. For instance, the pro-2 mutant

model plants. Additionally, metabolites in tomatoes are(TOMJPE2753-1), which harbors a mutation in the DELLA

highly sought after target molecules in industrial research.

gene, has contributed to research on parthenocarpy, thephenomenon of fruit development without pollination.

Stock
MICROBES⨋

Cultivars, wild species, and crossed lines: about 100 lines⨋

Individual mutant collections: about 2,500 lines⨋

EMS-treated bulked M3 seed set:
originated from about 5,500 lines⨋

T-DNA insertion lines: about 500 lines⨋

cDNA and full-length cDNA clones: about 270,000 clones

Unique Features
CELLS, DNA MATERIALSThe NBRP is home to the only center in the world that

Micro-Tom cultivation: Micro-Tom is amenable to high-density cultivation
because of its small size.

collects and makes available large mutant collections ofMicro-Tom cultivars and a T-DNA insertion line series (simple
T-DNA insertion, activation-tagging, and FOX hunting).

Upgrade and Maintenance Activities
Core Center
The University of Tsukuba will collect more detailed
phenotypic/genetic information on individual mutants toincrease their value to users.

Sub-Core Center
Meiji University will integrate our resource information
INFORMATION

with high-quality functional gene annotations into the
TOMATOMICS knowledge-base.

Popular Strains and Applications

Micro-Tom mutants: (clockwise from top left) flower-color, fruit-shape,
vegetative-shape, fruit-shape, bud-formation, leaf-shape, leaf-color, andvariegation.

Contact
Core Center: Institute of Life and Environmental
Sciences/Tsukuba-Plant Innovation
Research Center, University of Tsukuba

Micro-Tom (TOMJPF00001)
Micro-Tom is a dwarf tomato cultivar with excellent

24

Principal Investigator: Tohru Ariizumi
Email/URL: requestomato@t-pirc.tsukuba.ac.jp

Website: https://shigen.nig.ac.jp/chrysanthemum/index.jsp

Chrysanthemums

PLANTSANIMALS

Overview

line generated by repeated selfing of a self-compatible
mutant of Chrysanthemum seticuspe. In contrast to
the majority of the Chrysanthemum genus, including
cultivated chrysanthemum, which are autopolyploid andchrysanthemum, has interesting characteristics that

self-incompatible, Gojo-0 is diploid and self-compatible,

model plants do not have. However, the majority ofmaking it suitable for genetic analysis and manipulation,

the Chrysanthemum species are autopolyploid andsuch as isolation of recessive mutants. Gojo-0 is usedself-incompatible, which makes genetic analysis difficult.

as a platform to study the Chrysanthemum genus

NBRP facilities contribute to molecular genetic studies

because of the availability of whole genome sequencingof the Chrysanthemum genus by offering a variety of

information, transcriptome data, and DNA marker

interesting Chrysanthemum species and developing

information for this strain.

PLANTS

The Chr ysanthemum genus, including cultivatedunique resources based on a pure line of Chrysanthemum

seticupse, a diploid wild chrysanthemum, whose wholegenome sequence information is available.

Stock
Wild strains of Chrysanthemum sensu lato (Anthemideae)

MICROBES⨋

mostly native to Japan: about 500 strains⨋

Experimental lines including the model strain, mutants,
and interspecific hybrid lines: about 100 strains⨋

A BAC library of Chrysanthemum seticuspe:
about 100,000 clones

Unique Features

Wild-type and mutant Chrysanthemum seticuspe. Inflorescences of themodel strain Gojo-0 (left) and the shiboridama mutant (right).

CELLS, DNA MATERIALSThe NBRP is home to the only center that publicly
distributes one of the world’s largest wild chrysanthemum
collections. We offer the chrysanthemum model strain
and mutants developed at our center.

Update and Maintenance Activities
Core Center
Hiroshima University will preserve the chrysanthemum
collections, expand available mutants and update their
genetic information in reference to the Gojo-0 strain.

Sub-Core Center

Chrysanthemum repestre, a diploid Chrysanthemum species lacking ray
florets that grows on highlands in Japan.

Kochi University will collect wild strains and preserve

Popular Strains and Applications

Contact
Core Center: L aboratory of Plant Chromosome andGene Stock, Graduate School of Integrated

Gojo-0 strain

INFORMATION

Chrysanthemum plants for backup purposes.

Sciences for Life, Hiroshima University

The Gojo-0 strain was developed from Chrysanthemum

Principal Investigator: Makoto Kusaba

seticuspe, a diploid species native to Japan. It is a pure

Email/URL: akusaba@hiroshima-u.ac.jp25Website: https://shigen.nig.ac.jp/asagao/

Morning glories

PLANTSANIMALS

Overview

reference genome sample. The DNA clones we provide
were produced from this line. Because the transpositionsof endogenous transposons are suppressed in the line,

The Japanese morning glory (Ipomoea nil ) is useful forthe genome sequence has remained homogeneous.

research purposes because its generation time is less than
3 months and its size can be controlled by manipulating

Violet (Japanese: Murasaki)

PLANTSthe daylength. Research on this plant is increasing to take

This is the most requested line because it is a widely

advantage of the fact that it is a short-day plant, has vining

used standard line in various fields of plant research,capabilities and short one-day flowering time. The genome

including plant physiology. It has large-size organs andof I. nil is also uniform among lines, making it easy to identify

shows acute sensitivity to daylength.

the gene responsible for a mutation. We maintain a varietyof I. nil mutants originating in the Edo period, and new
mutations can be induced using endogenous transposons.

Stock
MICROBES⨋

Mutant and wild-type lines: about 2,500 lines⨋

Sibling species lines: about 450 lines⨋

cDNA clones: about 61,100 clones⨋

BAC clones: about 115,200 clones⨋

Petal-specific expression vectors and more

Unique Features
The NBRP is the only resource distributor in the worldStandard Japanese morning glory lines. Tokyo Kokei Standard (TKS, left)
and Violet (right).

that provides morning glory mutant lines originating in
CELLS, DNA MATERIALS

the Edo period, wild-type lines from around the world,
mutant and wild-type lines of closely related species, andvarious DNA resources.

Update and Maintenance Activities
Core Center
Kyushu University will publish detailed information about
our resources and their outstanding features to increase
the number of users.

Sub-Core Center
The National Institute for Basic Biology will add the
genome sequences of our mutant lines to elevate the
INFORMATION

genome database.

Popular Strains and Applications

Contact
Core Center: D epartment of Biological Science,

Tokyo Kokei Standard (TKS)

26

Some of the morning glory lines with available whole genome sequencing
information. Sequence comparisons have revealed the genes responsible
for the mutations carried by these lines.

Graduate School of Science, Kyushu University

This is a wild-type line selected by Dr. Yo Takenaka of

Principal Investigator: Eiji Nitasaka

the National Institute of Genetics that was used as the

Email/URL: asagao@kyushu-u.org

Website: https://shigen.nig.ac.jp/algae/?lang=en

Algae

MICROBESANIMALS

Overview

Ectocarpus (KU-1372) have been used in various research
fields because they have stable and good growth potential
and are easy to handle experimentally. Many diversified

Algae are an evolutionarily phylogenetically diverse

algal resources have been used for many years in specificgroup and are important primary producers in a variety

fields, such as research on algae-related environmentalof aquatic environments, including oceans, coasts,

issues, ecotoxicology, and biomass research.

The National Institute for Environmental Studies (NIES)

Successful whole-genome sequencing of some algae

provides diverse algae and protists covering 21 phyla andspecies has led to the wide use of these resources. Some77 classes, while Kobe University provides the major taxa

examples are Klebsormidium (NIES-2285), which has plantof seaweeds. Our algal resources have been used as

hormones in common with land plants, and Cyanidioschyzon

model organisms in photosynthesis research and a wide

(NIES-3377), a hot-spring alga. We have recently seen arange of research fields such as environmental research,steady increase in the use of our resources, not only for algal

biomass utilization and the development of medicines.

biomass research, but also in more applied fields such as

PLANTS

terrestrial waters and even extreme environments.

instrumentation and the development of medicines.

Stock
Microalgae and protists closely related to algae:

MICROBES⨋

about 2,700 strains⨋

Freshwater macroalgae, such as charophytes and more:
about 300 strains⨋

Seaweed strains comprising red, brown and green algae:
about 1,100 strains

Unique Featuresof which originate from Japan. The NIES collection also
includes model organisms such as Chlamydomonas.

Light micrographs of various microalgal resources preserved and provided
by NIES. Scale bars represent 10 µm.

Kobe University has the only culture collection in theworld that specializes in seaweed.

Update and Maintenance Activities
Core and Sub-Core Centers

CELLS, DNA MATERIALSNIES provides diverse groups of algal resources, most

NIES, as a core center, and Kobe University, as a sub-core
center, are working to cryopreserve the algal resources
and share useful information related to these resources
through international databases and social media.
to back up the core resources.

Popular Strains and Applications

Contact
Core Center: B iodiversity Division,

INFORMATION

Hokkaido University, as a sub-core center, will continue

Light micrographs of various seaweed resources preserved and provided
by Kobe University.

National Institute for Environmental Studies
Well-known algal resources such as Chlamydomonas

Principal Investigator: Masanobu Kawachi(NIES-2235), Chlorella (NIES-227), Euglena (NIES-48), andEmail/URL: kawachi.masanobu@nies.go.jp

27Website: http://nbrpcms.nig.ac.jp/paramecium/?lang=en

Paramecium

MICROBESANIMALS

Overview
Paramecium is one of the world’s largest unicellularstrain is also used as a natural host model for Legionella,
an intracellular bacterium.

P. bursaria Yad1g1N (PB031010B)

PLANTS

organisms. It is easy to culture and micromanipulate,P. bursaria harbors symbiotic Chlorella inside its cells

making it a useful model organism of eukaryotic cells(secondary symbiosis: intracellular symbiosis between

in a range of basic research studies, including thoseeukaryotic cells). A comparative analysis of the genes

examining endosymbiosis, the natural host of pathogens,

expressed between the Chlorella symbiotic strain

aging, motility, division, sexual cell recognition, learning,

Yad1g1N and the alga-free strain led to the identificationa n d wate r pu r i f i c ati o n. In ad d i ti o n, th e g e n o m eof multiple genes involved in secondary symbiosis.

sequences of the macronucleus (vegetative nucleus)
have been annotated for several species, and the range
of genetic approaches available for Paramecium isincreasing.

Stock
MICROBES⨋

24 out of 33 species that can be collected in the field⨋

Standard strains: 3 species, 6 strains⨋

Strains recommended for use: 24 species, 63 strains⨋

Representative strains of syngens and mating types of

P. caudatum: 15 strains
Unique Features
The NBRP is home to the only center in Asia thatCELLS, DNA MATERIALS

preserves and provides Paramecium resources. Our

Standard Paramecium (Paramecium caudatum).

collection is one of the largest in the world and includes
24 species of Paramecium spp., syngens and mating
types of P. caudatum.

Update and Maintenance Activities
Core Center
Yamaguchi University will continue to work on plannedcollection and progeny production, and the development
and improvement of cryopreservation methods to sustain
and add value to our resource.

INFORMATION

Popular Strains and Applications

Contact

P. caudatum dKNZ-12E (PC122029A)
P. caudatum is used as a representative Paramecium
strain because of its stable growth and phagocytic ability.

28

Paramecium bursaria. Symbiotic Chlorella autofluoresce as red granules.

Core Center: J oint Faculty of Veterinary Medicine,
Yamaguchi University

It has been used in conjugation studies with the same

Principal Investigator: Masahisa Watarai

syngen mating type O strain dKNZ-12O (PC121031A). This

Email/URL: watarai@yamaguchi-u.ac.jp

Website: https://nenkin.nbrp.jp/

Cellular slime molds

MICROBESANIMALS

Overview
Cellular slime molds are eukar yotic unicellular soil

the functional analysis of genes at the genome level havebeen conducted on all of these standard strains.

Genome Editing Vectors (pTM Vector Series)
As cellular slime molds are hemizygotes, analysis of geneaggregate to become multicellular and form fruiting

function using genome modification is straightforward inbodies composed of a spore mass and a stalk. They

these organisms. In addition, the high efficiency of gene

have been used as a model organism in cytology,transfer also makes it feasible to produce the desired

embryology, and mathematical biology, and are also

genome-edited strains. Studies have used these genome

attracting attention as a novel drug discovery resource.

editing vectors to examine the molecular functions ofGenome-edited strains can be easily produced, and avarious genes.

PLANTS

amoebae that feed on bacteria and, upon starvation,

wide variety of expression vectors are available.

Stock
⨋

Genetically mutated or transformed lines:
about 1,300 strains
Wild strains collected from around the world:

MICROBES⨋

about 420 strains⨋

Gene disruption vectors, expression vectors:
about 860 vectors

Unique Features

The fruiting body of Dictyostelim discoideum . After 24 hours understarvation conditions, the cellular slime molds form a fruiting body.

The NBRP is home to one of only two cellular slime moldresource centers in the world and the only one based in
CELLS, DNA MATERIALS

Asia. We are the only institution that stores and provides
genome-edited vectors and genome-edited strains.

Update and Maintenance Activities
Core Center
The University of Tsukuba will endeavor to improve
our training courses and expand our collection to raiseinternational recognition, improve user experience, andthe number of users.

Popular Strains and Applications
and AX4 of Dictyostelium discoideum

Contact

Dictyostelium discoideum is the most commonlyused standard species for cellular slime mold researchworldwide. The standard strains A X 2, K A X3, andCore Center: Institute of Life and Environmental Sciences,

INFORMATION

Strains AX2, KAX3,

Cellular slime mold (Dictyostelium discoideum) in the aggregation phase.
Cells form aggregates about 10 hours after being starved.

University of Tsukuba

AX4 can be cultured without bacteria and have whole

Principal Investigator: Hidekazu Kuwayama

genome data available. Research studies focusing on

Email/URL: kuwayama.hidekazu.fu@u.tsukuba.ac.jp29

Website: http://muses.muses.tottori-u.ac.jp/facilities/FMRC/english/index.html

MICROBES

Mushrooms

ANIMALS

Overview

agricultural sciences. Some examples are listed below.

Neolentinus lepideus “Matsuouji” (TUFC 31432)

Mushrooms are used in a variety of fields. For example,

Culture extracts of this strain contain novel tyrosinase

they are widely used as traditional herbal medicines

inhibitors that are a useful skin whitening agent (Patent

and as bioremediation agents to exploit their specificnumber: JP7054156).

PLANTS

decomposing enzymes. Mushrooms are promisingresources for medicines, pesticides, and other products,

Phlebia tremellosa “Siwatake” (TUFC 11737)

and may be a source of novel bioactive compounds that

Culture extracts of this strain contain novel tyrosinase

differ from those produced by molds and other microbes.

inhibitors that are a useful skin whitening agent (Patent
number: JP7054156).

Stock
Our strains belong to diverse ecological groups including

Daedalea dickinsii “Hourokutake” (TUFC 31876)

wood decomposing, mycorrhizal, edible, and poisonous

Culture extracts of this strain contain growth inhibitors

mushrooms.

against Helicobacter pylori and Campylobacter jejuni⨋

Wild mushrooms collected mainly in Japan:(Patent application number: 2019-208982).

MICROBES

9,125 strains of 1,817 species belonging to 588 genera(as of May 2022)

Unique Features
NBRP’s Tottori University Fungal Culture Collection (TUFC)
is one of the largest mushroom culture collections in theworld. We have several strains of wood decomposers
including agaricoids, cor ticioids, polyporoids, and

CELLS, DNA MATERIALS

auriculoids. We also have various rare mycorrhizal strains,whose isolation, cultivation, and preservation are difficult.

Preparation of cryotubes containing mycelial samples.

Update and Maintenance Activities
Core Center
Tottori University hopes to raise the profile of its resource
center and improve user experience. The university will
also engage in publicity activities and continue to update
its strain database.

Popular Strains and Applications
INFORMATIONTUFC contains wild strains of edible mushrooms, such as“Shiitake” and “Matsutake”, and industrially useful strains

Contactthat produce lignin or cellulose decomposing enzymes,
ecologically important or rare species and taxonomicallyimportant ex-type strains. The use of these strains in

30

Cultivation of Gymnopilus junonius.

Core Center: Fungus/Mushroom Resource and ResearchCenter, Faculty of Agriculture, Tottori University

both basic and applied research has generated many

Principal Investigator: Tsuyoshi Ichiyanagi

patents and scientific papers in the fields of medical and

Email/URL: fmrc-collection@mail.muses.tottori-u.ac.jp

Website: https://yeast.nig.ac.jp/yeast/

MICROBES

Yeast
ANIMALS

Overview

Auxin-inducible degron system (about 100 resources)
Auxin-inducible degron (AID) technology, which was
originally developed by Japanese researchers, is aunique protein knockdown system that enables a protein

experimental methods and research resources haveof interest to be depleted in a half-life of less than 30

been developed for yeast for genetic, biochemical,

minutes simply by adding auxin to the culture medium.

and molecular biology studies, including recombinant

Since the AID system is the most effective tool for in vivo

DNA technology. Yeast research has contributed to ourprotein knockdown, AID is an in-demand technology

understanding of many molecular mechanisms importantfor studying the function of essential genes and in drug

for life, including those involved in the cell cycle anddiscovery research.

PLANTS

Yeast is a major model organism in life sciences. Various

autophagy. Because of the availability of a comprehensive
range of genomic, transcriptomic, proteomic, and other-omic information, yeast research is expected to play aleading role in post-genome research.

Stock
Fission yeast strains: about 16,000 strains⨋

Fission yeast plasmids: about 60,000 clones⨋

Budding yeast strains: about 20,000 strains⨋

Budding yeast plasmids: about 7,000 clones

Unique Features
The NBRP is the only resource center in the world to have

MICROBES⨋

Fluorescence micrograph of the fission yeast Schizosaccharomyces pombe(Image courtesy of Dr. Masashi Yukawa, Hiroshima University).

large-scale fission yeast resources and original budding
CELLS, DNA MATERIALS

yeast resources created mainly by Japanese researchers.

Update and Maintenance Activities
Core Center
Osaka Metropolitan University will continue to collect,preserve, and provide fission yeast.

Sub-Core Centers
Hiroshima Institute of Technology will continue to collect,preserve, and provide budding yeast. Hiroshima University
will continue to preserve the resource for backup purposes.

Fission yeast GFP-fusion gene expression strains

Contact

This is a set of fission yeast strains (about 1,000 strains) inwhich each gene is chromosomally tagged with GFP. Thesestrains can be used to observe the subcellular localization ofCore Center: Yeast Genetic Resource Center, Graduate School

INFORMATION

Popular Strains and Applications

A differential interference contrast image of the budding yeast Saccharomyces
cerevisiae.

of Science, Osaka Metropolitan University

a protein of interest and is very useful for analyzing protein

Principal Investigator: Taro Nakamura

function. We receive many requests for these strains.

Email/URL: contactus-yeast@shigen.info31

Website: https://jcm.brc.riken.jp/

General microbes

MICROBESANIMALS

Overview

Commensal bacterial strains of humans andother animals
Commensal bacteria in the gut, oral cavity, and skin,

PLANTSThe Japan Collection of Microorganisms (JCM) provides

and lactic acid bacteria and/or bifidobacteria have adiverse microbial strains of bacteria, archaea, yeast,

significant impact on our health. The strains we provide

and filamentous fungi. Our operations are certified by

are used for a variety of studies, such as the investigation

the quality management ISO 9001 system, and we willof their ef fects on immune function, prevention ofcontinue to ensure the high credibility of our resources

disease, obesity, and beauty. Many of these strains areand services. We also provide highly valuable microbial

anaerobes and a large number are unique commensal

strains that have been reported in scientific publications.

strains available only in JCM.

JCM strains are widely used and contribute to a varietyof research fields related to environmental and healthscience as well as general microbiology.

Stock
MICROBES⨋

Bacteria: about 21,000 strains⨋

Archaea: about 1,000 strains⨋

Fungi: about 8,800 strains⨋

Type or type-derived strains: about 10,000 strains

Unique Features
JCM has one of the world's largest number of prokaryotic
and yeast type strains. All available type strains are well

The nanoarchael strain Nanobdella aerobiophila JCM 33616 (left, twosmall cells) associated with the cell surface of the host archaeal strain
Metallosphaera sedula JCM 33617 (middle, large cell).

characterized. We receive deposits of a large number
CELLS, DNA MATERIALSof strains corresponding to novel species annually fromboth domestic and overseas researchers.

Update and Maintenance Activities
Core Center
JCM will maintain reliable quality of our microbial strains
and will continue to update genome sequencing data to
add value to our strains.

Popular Strains and Applications
INFORMATION

Archaea and extremophile strains
These strains inhabit various environments on Earth and areContact

actively used in studies to understand specific environments,
the evolution of life, their unique metabolism, and in appliedCore Center: M icrobe Division/Japan Collection ofscience, such as investigations of thermophilic enzymes.

Microorganisms, RIKEN BioResource

We have one of the world's largest number of archaeal and32

Legionella pneumophila subsp. pneumophila JCM 7571, an isolate fromhuman lung.

Research Center

extremophile strains, including those that are very difficult to

Principal Investigator: Moriya Ohkuma

cultivate and not available from other resource centers.

Email/URL: inquiry.jcm@riken.jp

Website (left: Escherichia coli) : https://shigen.nig.ac.jp/ecoli/strain/(right: Bacillus subtilis) : https://shigen.nig.ac.jp/bsub/

Prokaryotes

MICROBES

Overview

ANIMALS(Escherichia coli, Bacillus subtilis)

recent technology that has become popular because it isinexpensive, easy to use, and convenient. This resource
contributes to prokaryote research and also to various life

There is a wealth of available biological knowledge on andscience fields.

experimental methods for E. coli and B. subtilis. As a result,a number of extremely important E. coli and B. subtilis

KEIO collection and BKE library
These are collections of single gene knockouts of E. coliof single gene knockout mutants of E. coli, and the BKE

and B. subtilis, respectively. We offer individual strains

library of B. subtilis, the contents of which are recognizedor the whole set. These resources can be used foras international standards for genetic analyses ofcomprehensive screening, functional analysis of a gene

Gram-positive and Gram-negative bacteria. Bioresourcesof interest, or to produce functional biomaterials.

PLANTS

resources exist. Among these is the KEIO collection, a set

for both E. coli and B. subtilis are important for advancing
science and technology in both medical and industrial fields.

Stock
⨋

Original collection of mutant strains
Single gene knockout collection (E. coli KEIO and B.

MICROBES⨋

subtilis BKE collections)⨋

Large-scale chromosomal deletion mutants (KHK collection)⨋

Gene clone collection (ASKA strains)⨋

Cloning and expression vectors (467 vectors)

Unique Features

E. coli cells. A phase contrast image showing nucleoids stained with DAPI
(magenta).

The NBRP provides E. coli K12 derivatives and B. subtilis
CELLS, DNA MATERIALS

168 derivatives to researchers worldwide who work in thelife science field. Collections of single gene knockouts ofE. coli and B. subtilis are also available. Each collection
comprises approximately 4,000 strains.

Update and Maintenance Activities
Core Center
The National Institute of Genetics will continue to collect,preserve, and provide E. coli and B. subtilis strains, andperform whole genome sequencing of these strains tovalidate their genotype.

B. subtilis cells. (Left) Differential interference image. (Right) Cell membranestained with FM4-64.

Sub-Core Centerfor backup purposes.

Popular Strains and Applications

Contact
Core Center: D epar tment of Gene Function andPhenomics, National Institute of Genetics

INFORMATION

Kyushu University will continue to preserve bioresources

Principal Investigator: Hironori NikiiVEC strains (ME9806)

Email/URL: genkaku@nig.ac.jp

The E. coli iVEC strain is amenable to seamless cloning, a33

Website: https://pathogenic-microbes.nbrp.jp/

Pathogenic eukaryotic microbes

MICROBESANIMALS

Overview

major problem in recent years. These resistant strains areused to elucidate the genes involved in resistance andthe mechanisms of resistance by comparing resistant

The pathogenic eukaryotic microbe collection at theand susceptible strains isolated from clinical isolates.

NBRP consists mainly of reference strains for major
pathogenic fungi and actinomycetes and clinical strains.
PLANTS

These strains are widely used to evaluate new drugs,

Luciferase-expressing strains of Trypanosoma
cruzi (Tc143–Tc147)

to elucidate antifungal resistance mechanisms, andWe provide five luciferase-expressing strains generated

to discover useful substances. We also provide morefrom different origins. Trypanosoma cruzi is a protozoan

than 40 pathogenic protozoa species. These are usedparasite that causes Chagas disease. It is difficult toto establish infectious animal models, evaluate thedetect at the intracellular parasitism stage in the host.

quality, safety and effectiveness of drugs, study parasitic

Luciferase-expressing strains can be detected throughmechanisms and as positive controls in clinical tests.

luciferase activity.

Stock
⨋

Fresh clinical isolates of fungi, highly pathogenic fungi species

MICROBES

and major mycosis-causing fungi: about 15,000 strains⨋

Standard and clinical strains of pathogenic actinomycetes,mainly of the Nocardia genus: about 2,700 strains⨋

Pathogenic protozoa: about 370 strains

Unique Features
The NBRP has the highest quality and largest number ofclinical isolates of pathogenic fungi and actinomycetes

Aspergillus fumigatus. Typical fungal species that causes mycoses.

CELLS, DNA MATERIALS

among the bioresource centers around the world. We
have also preserved more than 40 protozoan species. In
particular, we boast the largest number of Trypanosoma

cruzi strains in the world.
Update and Maintenance Activities
Core Center
Chiba University will continue to collect and provide
clinical strains and drug-resistant information.

Sub-Core Center
Nagasaki University will continue to collect transgenic
protozoa and hybridoma cells that produce anti-pathogenic
INFORMATION

protozoan antibodies.

Popular Strains and Applications

Contact
Core Center: Medical Mycology Research Center, Chiba University

Azole drug-resistant Aspergillus fumigatus andterbinafine-resistant Trichophyton spp.

The emergence of drug-resistant fungi has become a34

Trypanosoma cruzi . Giemsa staining of trypomastigotes (arrow) andamastigotes (arrowhead) cultured in vitro.

Principal Investigator: Takashi Yaguchi
Email/URL: bioresource@ml.chiba-u.jp
protozoa@tm.nagasaki-u.ac.jp

Website: https://pathogenic-bacteria.nbrp.jp

MICROBES

Pathogenic bacteria
ANIMALS

Overview

research into pathogenic mechanisms and gene marker
and antigen analysis for pathogen detection. High-impact
papers have been published using these strains.

There is no end to humankind's fight against infectious

Respiratory pathogens such as Legionella andreduce quality of life in later life. However, it can be difficult

mycobacteria, enteric infectious disease pathogens

for university researchers and companies to obtain

Target bacterial species, species requiring discrimination

pathogenic bacteria to study pathogenic mechanisms,

from target bacterial species, and their related species

develop therapeutic and diagnostic agents, and examine

are used to develop diagnostic methods and to test

the pathogenic bacteria themselves. The NBRP conserves

and verify new methods during the development ofand provides a wide variety of pathogenic bacteria fordiagnostic agents and identification kits. These species

research purposes.

are particularly essential for assessing the performance

PLANTS

diseases, which can be life-threatening and significantlyof methods for diagnosing infectious diseases that involve

Stock
⨋

many pathogens and require prompt diagnosis.

Pathogenic bacteria that cause opportunistic infection;biosafety level 3; class II, III, and IV pathogens under
MICROBES

the Infectious Diseases Control Law; non-pathogenic
bacteria related to these pathogens: about 4,800 strains⨋

Human enteropathogenic bacteria including Vibrio

parahaemolyticus, V. cholerae, and Shiga toxin-producing
Escherichia coli (about 12,000 strains in total): 1,286 strains
Unique FeaturesNBRP Pathogenic Bacteria is home to the only resource
specific pathogens and pathogens of rare infectious

Vibrio parahaemolyticus. This pathogen causes seafood-associated acutegastroenteritis.

diseases. We also have a rich collection of wild strains ofrespiratory infectious and enteropathogenic bacteria.

Update and Maintenance Activities
Core Center
Gifu University will revamp the resource website andCELLS, DNA MATERIALS

center in Japan with about 350 genera of bacteria, including

catalog database to improve visibility and user experience.

Sub-Core Center
Osaka University will continue to analyze the genomes ofthe strains in our culture collection.

Contact
Core Center: C enter for Conservation of Microbial

Human enteropathogenic bacteria

Genetic Resource, Institute for Advanced

Vibrio parahaemoly ticus and enterohemorrhagic

Study, Gifu University

Escherichia coli isolated from outbreaks of foodborne

Principal Investigator: Kaori Tanaka

diseases in Japan are frequently requested and used for

Email/URL: g_cmr@gifu-u.ac.jp

INFORMATION

Popular Strains and Applications

Poprhyromonas gingivalis. A red-complex bacteria that causes periodontitis
also known to be associated with various chronic diseases.

35

Website: http://www.tm.nagasaki-u.ac.jp/nbrp-virus/en/

Human pathogenic viruses

MICROBESANIMALS

Overview

Influenza A virus strains
Influenza A virus is one of the most popular zoonotic
viruses and is responsible for causing seasonal influenza

PLANTSA few hundred virus species are thought to be pathogenic

each year. A new type of influenza virus emerges every

to humans. In addition, many novel viruses have beenfew decades and causes an influenza pandemic. Our

identified in the 21st century. Each species has a variety

resources have been used to analyze the mechanismsof strains that target different animal hosts, organs,involved in virus growth and pathogenicity, to study viraltissues, and have different cell specificity, pathogenicity

epidemiology, and to develop diagnostics and antivirals.

and antigenicity. A variety of these virus strains have beenused to analyze virus pathogenicity, antigenicity andepidemiology, and to develop diagnostics and antivirals.

Stock
⨋

Number of species: 28 species⨋

Wild and mutant strains: 1,482 strains⨋

cDNA clones: 17 strains

MICROBES

Unique Features
The NBRP has one of the largest number of influenza
viruses, herpes simplex viruses, gastroenteritis viruses
and arboviruses. We are the only facility that distributes
human pathogenic virus strains in Japan.

Influenza A virus. Two viral glycoproteins, HA and NA, can be seen on thevirion surface.

Update and Maintenance Activities
CELLS, DNA MATERIALSCore Center
Nagasaki University will expand its collection anddatabase, and engage in promotional activities toincrease the number of requests for resources.

Sub-Core Centers
The University of Tokyo, Hokkaido University, Osaka
University, and RIKEN will continue to diversify the collection
and increase the number of requests for resources.

Popular Strains and Applications
INFORMATIONSARS-CoV-2 strains
SARS-CoV-2 is the virus that causes COVID-19. We

Contact

have isolated many SARS-CoV-2 strains from clinicalspecimens. Whole genome sequencing has been
performed on most of these isolates. These resources
have been used to analyze mechanisms involved in virus

36

Herpes simplex virus. Progeny virus can be seen budding from an infectedcell.

Core Center: N ational Research Center for the Control
and Prevention of Infectious Diseases,
Nagasaki University

growth and pathogenicity, to study viral epidemiology,

Principal Investigator: Jiro Yasuda

and to develop diagnostics and antivirals.

Email/URL: virus-r@tm.nagasaki-u.ac.jp

Website: https://cell.brc.riken.jp/en/hcb

CELLS
DNA MATERIALSHuman cord blood cells
ANIMALSof a baby. After thawing, one tube usually contains

Overview

approximately 1 x 10 5 cells and more than 90% of thecells are CD34-positive. The CD34+ CB cells can be

Cord blood (CB) contains an abundance of the mostused for hematopoietic stem cell transplantation research

immature hematopoietic stem cells, and is widely used

and also regenerative research where the cells act as ain medical and biological studies, including regenerative

source of iPS cells.

genetics research involving iPS cells. The NBRP provides

Frozen CB mononuclear cells (MNC) (Small volume

researchers with quality-assured human CB samples that＞ 1x107/tube)

will contribute to the advancement of life science and

MNCs are the most experimentally accessible cells for

medical research. The collected CB is processed into

researchers. These cells are useful for studies of immune

several frozen samples at The University of Tokyo’s Cell

cells such as T/B cells, macrophages, NK cells, Tregs,

Resource Center (IMSUT-CRC) and made available tomast cells, and dendritic cells. MNCs can also be used toresearchers upon request.

extract mitochondria, exosomes, and nucleic acids such

PLANTS

medicine, drug discovery, immunology, infection, andas RNA under various conditions, and for genomic andStockproteomics research.
7

Frozen CB mononuclear cells (Small volume > 1x10 /MICROBES

⨋

tube)
⨋

Frozen CB mononuclear cells (Large volume > 1x108/bag)⨋Frozen CB nucleated cells ( > 3x108/bag)⨋Frozen CB CD34 positive cells ( > 1x105/tube)

Unique Features
CB contains the youngest human somatic stem cells
miRNA, exosomes and mitochondria can be extracted
from CB.

Update and Maintenance Activities

Frozen CD34-positive cord blood cells purified with magnetic beads (MACS).
Cord blood cells are processed into mononuclear cells and sorted by
selecting for CD34-positive cells (Purity >90%) and then frozen.

Core Center
The University of Tokyo IMSUT Hospital will continue tocollect CB from gynecology hospitals and process it into
several types of frozen samples using aseptic techniques.

Contact

CELLS, DNA MATERIALS

of fetal origin. Frozen living cells, DNA, RNA, proteins,

Sub-Core Center
The RIKEN BioResource Research Center will continue
to provide various frozen CB materials collected by the

Science, University of Tokyo
Principal Investigator: Tokiko Nagamura-Inoue
Email/URL: cellqa.brc@riken.jp

Popular Strains and Applications

INFORMATION

University of Tokyo.

Core Center: IMSUT Hospital, The Institute of Medical

Frozen CB CD34-positive cells (CD34) ( > 1x105/tube)
These frozen CD34-positive cells were collected using
magnetic beads (MACS) within 48 hours after delivery

37

Website: https://cell.brc.riken.jp/

CELLS
DNA MATERIALSHuman and animal cells

ANIMALS

Overview

innovation in the field of disease research.

Human cancer cell lines

PLANTS

Cultured cells, particularly immortalized cell lines, are a

The HeLa cell line was generated in 1952 from cervicalcommon resource used by many researchers around thecancer cells, and is still used in many research studies

world. They can be cryopreserved and expanded, and cantoday. Since HeLa, many other cancer cell lines havethus be used whenever necessary. They have been used inbeen generated around the world. Those cancer cell lines

many basic and medical science applications, including as

have contributed enormously to the basic sciences andfactories to produce proteins. Furthermore, somatic stem

medical sciences.

cells, embryonic stem (ES) cells, and induced pluripotent
stem (iPS) cells are increasingly being used in the field of
regenerative medicine as cell transplantation therapy.

Stock

MICROBES⨋

Human cell lines (e.g., cancer cell lines): about 4,300 lines⨋

Animal cell lines (e.g., mouse, rat): about 3,800 lines⨋

Human and animal ES cell lines: about 4,200 lines⨋

Human and animal iPS cell lines: about 1,500 lines⨋

Human disease-specific iPS cell lines: about 3,700 lines

Unique Features
Because iPS cell technology was developed in Japan by
Dr. Shinya Yamanaka, many national projects in Japan

HeLa cells: the world’s first cancer cell line. The line was derived fromcervical cancer cells in 1952 and is still widely used in research today.

provide financial support for research using iPS cells. As
CELLS, DNA MATERIALS

a result, the human disease-specific iPS cell bank at the
RIKEN BioResource Research Center (BRC) is now theworld’s largest of its kind.

Update and Maintenance Activities
Core Center
The Cell Engineering Division at RIKEN BRC is planningto establish a “patient blood cell bank” that will contain acollection of blood cells from patients with various diseases.

Popular Strains and Applications
INFORMATION

Human disease-specific iPS cells
It is impossible to extract brain cells from patientsContact

suffering from brain diseases. However, thanks to iPS
technology, disease-specific iPS cells can be generated
from a patient’s blood cells and subsequently induced to38

Mycoplasma test. Mycoplasma in infected cells is visualized as spots in thecytoplasmic area.

Core Center: C ell Engineering Division,
RIKEN BioResource Research Center

differentiate into brain cells for use in basic research on

Principal Investigator: Yukio Nakamura

the disease and drug discovery. iPS technology is driving

Email/URL: cellbank.brc@riken.jp

Website: https://dna.brc.riken.jp/

DNA-related materials

CELLS
DNA MATERIALSANIMALS

Overview

cellular responses to external stimuli because they enable
the visualization of vital phenomena such as cell cycle
phase and organelle behavior in real time.

Genetic material such as plasmids, expression andreporter vectors and comprehensive clone sets of cDNAs

Recombinant lentivirus vector plasmids
We have collected a large number of plasmids developed byfundamental bioresources for life science research.

the late Dr. Hiroyuki Miyoshi and related modified plasmids.

Genetic material is now widely used in a variety life

These plasmids can be efficiently transferred into the genes

science fields ranging from basic research aiming toof cultured cells and individual animals, and are used toanalyze gene function and the mechanisms involved instudy cancer and animal development. More than 420

regulating gene expression, to applied research studies

papers have been published using the plasmids we supply.

PLANTSand genomic DNAs are one of the most important andto develop therapies and drugs and produce materials.

Stock
⨋

Research tools for imaging and genome editing:about 3,400 items
Human cDNA and genomic DNA clones: about 600,000 items⨋

Mouse cDNA and genomic DNA clones: about 350,000 items⨋

Animal cDNA and genomic DNA clones: about 1,030,000 items⨋

Genomic DNA of microorganisms and mouse strains from

MICROBES⨋

RIKEN BRC and more.

Unique Features
The RIKEN BioResource Research Center (BRC) is the only

Fucci-transfected cells. Fucci can be used in living cells to visualize cells that arein the G1 phase of the cell cycle in red, the G1/S phase in yellow, and the S/G2/M
phase in green (Photo provided by Dr. Asako Sakaue-Sawano, RIKEN CBS).

CELLS, DNA MATERIALS

organization in Asia that actively collects, preserves andprovides genetic material developed by researchers. We also
provide large-scale clone sets produced by national projects.

Update and Maintenance Activities
Core Center
In response to rapid advancements in research methods,
RIKEN BRC’s Gene Engineering Division is planning tobuild a system that can quickly collect and provide new
genetic material.

The pEF-BOS vector. This is one of the plasmid vectors available from ourinstitution. Regardless of the type of mammalian cell, this vector can strongly
express the inserted gene in the host cell.

Imaging resources (fluorescence and luminescence)Contact

We collect fluorescent proteins and luminescent enzymes
from various organisms, and biological imaging resources
developed based on these molecules with desirable

Core Center: G ene Engineering Division,

INFORMATION

Popular Strains and Applications

RIKEN BioResource Research Center

properties such as high brightness and multicolored

Principal Investigator: Yoshihiro Miwa

emissions. These resources are useful for studying

Email/URL: dnabank.brc@riken.jp39

Website: https://nbrp.jp/en

NBRP Information Center

INFORMATIONANIMALS

Overview

Genome Information Service
In cooperation with the NBRP core and sub-core
centers, we have developed a genome browser and

PLANTSOne of the biggest hurdles experienced by individuals

homology/key word search engine. The database

who want to use biological/genetic resources is notthat is linked to these tools contains the results of theknowing where and what kind of resources are available.

NBRP Value Addition Program, including those for

The NBRP Information Center provides information on

the following bioresources: Medaka (Oryzias latipes),

NBRP resources to researchers all over the world via

Xenopus, mice, quails, rice, wheat, barley, morning glory,

the internet. We develop resource databases containing

Lotus japonicus, Escherichia coli and Bacillus subtilis.

resource phenotype, genotype, and other related

We hope to expand on the genomic and other omics

information, and search and order systems for theseinformation in this database in the coming years.

databases. We have also developed genome browsers
for each resource and resource-related literature
databases. Our aim is to create a comprehensive
database of NBRP resources.

Services/Products
MICROBES⨋

Resource databases/websites: 25 organism species⨋

Organisms with publicly available genome information:
about 10 species⨋

Comprehensive resource search: about 6.5 million items⨋

Resource-related literature database: about 50,000 items

Unique Features

RRC: a database of published papers that have used NBRP bioresources.
The figure shows that NBRP bioresources have been used in a variety of
research studies around the world.

The NBRP Information Center provides a one-stop service
CELLS, DNA MATERIALS

to locate and purchase desired bioresources. Based on
open data and open access policies, we are making ourdatabases and websites easy for anyone to access toensure that all resource information is readily available.

Update and Maintenance Activities
The National Institute of Genetics plans to standardize
all available information by converting all databases to
RDF format to ensure the information can be used acrossorganisms.
Information Center Server System. Our server stores data on approximately
6.5 million bioresources.

INFORMATION

Popular Services/Products
Research Resource Circulation (RRC)

Contact

RRC is a database of published papers and patents thathave used NBRP resources. Users can freely register the
bibliographic information of any publications that haveused NBRP resources.

Core Center: D epartment of Informatics,
National Institute of Genetics
Person in Charge: Shoko Kawamoto
Email/URL: https://nbrp.jp/en/contact-en/40Website: http://www.shigen.nig.ac.jp/gain

INFORMATIONANIMALS

Great Ape Information Network(GAIN)
Overview

Apes, the most closely related animals to humans, arecritical for academic research. However, all species of apes
are endangered and research into these animals requires
a variety of considerations. GAIN is building a database of
PLANTS

all great and small apes (chimpanzees, bonobos, gorillas,orangutans and gibbons) kept in Japan. We collect andmanage available information on non-invasive samples(such as those derived from dead bodies) and informationon their background, family history, and genome, among
other data, and make them available for shared use by
researchers to promote academic research.
Collect information on individual apes kept in zoos⨋

Manage real-time databases of information on individual apes⨋

Provide information on the availability of non-invasive samples⨋

Provide support for various procedures during sample collection

Core Center: Wildlife Research Center, Kyoto University
Person in Charge: Satoshi Hirata
Email/URL: gain-core@wrc.kyoto-u.ac.jp

Website: https://gbif.jp/en

INFORMATION

Overview
Humans benefit from biodiversity in many aspects of ourlives on Earth, ranging from our food, clothing, and shelter

CELLS, DNA MATERIALSJapan Initiative for Biodiversity
Information (JBIF)MICROBES⨋

Contact

to economic activities. To maintain biodiversity, we need toknow how it works and how to conserve it. JBIF (formerly
GBIF Japan Node) is working to consolidate and disseminate
information on biodiversity in Japan both domestically andwith the world and freely viewed and used by anyone.
⨋

Release specimens/observation data through theContact

GBIF/JBIF website⨋

Promote the use of biodiversity information

Core Center/Sub-Core Center: National Museum of Nature⨋

Collaborate with museums and ecosystem observation networks

and Science and National Institute for Environmental Studies⨋

Provide information on species names and promote DNA

People in Charge: Utsugi Jinbo and Hiroya Yamano

barcoding

Email/URL: https://gbif.jp/en/contact

INFORMATION

internationally, to ensure that the information can be shared41

Website: https://idenshigen.jp/en/

Access and Benefit-Sharing(ABS)

INFORMATIONANIMALS

Overview
To access and use foreign genetic resources forresearch, it is important to abide by the relevant laws andregulations of the country providing the resources. Basedon the Convention on Biological Diversity, the Nagoya
PLANTS

Protocol on Access and Benefit-Sharing took effect in2014 to regulate the sharing of benefits arising from theuse of genetic resources between a provider country anda user country. The ABS support team provides a rangeof services to help researchers navigate the different laws
and regulations among provider countries.
⨋

Provide advice on ABS-related inquiries from researchers⨋

Provide ABS-related information and learning tools⨋

Organize workshops on ABS and other relevant rules⨋

Participate in relevant international conferences

Contact
Core Center: A
 BS Support Team for Academia, NIG
INNOVATION, National Institute of Genetics
Person in Charge: Mutsuaki Suzuki

MICROBES

Email/URL: abs@nig.ac.jp
Website: https://nbrp.jp/en

Public relations

INFORMATIONCELLS, DNA MATERIALS

Overview
The Public Relations team coordinates events andcampaigns to encourage life science researchers to use
the bioresources collected, preserved, and provided
by the NBRP core and sub-core centers. We are also
committed to raising awareness of the project among
the general public, including students and young people.
In doing so, we strive to connect the NBRP core and

INFORMATION

sub-core centers with our stakeholders.
⨋

Coordinate exhibits⨋

Manage the NBRP website⨋

Create posters and brochures⨋

Organize eventsContact
Core Center: N
 BRP Public Relations Office,National Institute of Genetics
Person in Charge: Ayumi Koso
Email/URL: https://nbrp.jp/en/contact-en/42Website: https://www.m-kenshou.org/

INFORMATION

Overview

ANIMALS

Human resource development for
promoting external verification ofanimal experimentation
management take a course comprising our educational
materials, and two mock written and on-site exams thatuse multiple simulation materials. We evaluate the reports

The Fundamental Guidelines for the Conduct of Animaland grant provisional qualification only to those who meet

Experiments set forth by the Ministry of Education,certain achievement criteria. After gaining additional work

Culture, Spor ts, Science and Technology (ME X T )experience in external verification, the candidates are

requires that research institutions be socially transparent

officially certified as verification specialists.

To this end, the Human Resource Development for

Educational support

Promoting External Verification of Animal Experimentation

We also support internal animal committee activities andproject aims to promote and raise awareness of theeducation and training activities for institutions by, forexternal verification system for animal experiments. The

example, distributing the free educational video "Animal

NBRP is working to improve the verification system,

Experiments are Managed by Institutions" in Japanese,increase the rate of external verification, and develop

English, and Chinese.

PLANTSby undergoing external verification of animal experiments.

related educational materials.

Achievements
Number of external verifier experts trained:

MICROBES⨋

22 (in 2015) to 101 in (2020)⨋

Total number of institutions that have undergone externalverification: 111 (in 2015) to 237 (in 2020, including 14
institutions outside the jurisdiction of MEXT)⨋

Total number of external verifier participants: about 108

Unique Features

Participants receiving individual consultation for external verification.

CELLS, DNA MATERIALSThe NBRP holds briefing sessions and individual
consultations for institutions that conduct animal
experiments to support them through external verification
inspections. By the 2020 academic year, a total of 704
institutions (1,286 individuals) had participated in ourbriefing sessions and 141 institutions had received
individual consultations.

Update and Maintenance Activities
We will continue to support qualified external verification
specialists and diversify our educational materials for
institutions that conduct animal experiments.

The opening frame of the Animal Experiments are Managed by Institutions
educational video in English.

Contact

Hands-on training program for external verification

Core Center: J apanese Association for Laboratory Animal

specialists

INFORMATION

Popular Services/Products
Science

Potential specialists with a wide range of experiences

Person in Charge: Chihiro Koshimotoin animal experimentation and laborator y animal

Email/URL: jinzaiikusei@jalas.jp

43

NBRP scheme

NBRP Core and Sub-Core Centers

MEXT
Award Grant

Life Science Committee
ReportStrategic Working Group
Consult

NBRP Evaluation Committee

InstructManageNBRP Oﬃce

Evaluation
Selection

Assist & Inspect

AdviceAssistInstruct, Consult

Value Addition
Subprogram
Technology
Development
Subprogram

Report Publications &Deposit Bioresources

Information Center
Program Steering
Committee

Information Center
ProgramRequest要望

User Community

ReportConsult

Resource Center
Program Steering
Committee

Resource Center
Program

Provide Bioresources
and their AssociatedInformation

ReportReportRecommend

Council for Science andTechnology Subdivision on
Research Planning and Evaluation

ManageGuide & Advice

Working GroupPD ／ PO
Program Director
Program Oﬃcer

Steering Committee
Chairperson Meeting

NBRP Program Director (PD)The PD coordinates the operation of the project and facilitates the collaboration between the programs.
氏

名Yuji Kohara

Director, Database Center for Life Science,
Research Organization of Information and Systems

所属

NBRP Program Officer (PO)The POs assist the PD and facilitate the operation of the programs.
氏名Yuichi Obata

Satoshi Tabata

Tetsuya Hayashi

44

所属

Honorary Scientist, RIKEN / Senior Visiting Scientist, RIKEN BioResource Research Center (BRC)
Vice President / Director, Kazusa DNA Research Institute

Professor, Graduate School of Medical Sciences, Kyushu University

List of NBRP organizations
Resource Center Program
Bioresource category＊

Aged mice

○

Mice

Rats
Japanese macaque
Chickens & Quails
Clawed frogs & Newts

Zebrafish○

Atsushi Yoshiki○

Masahide Asano

Caenorhabditis elegans
Arabidopsis
Rice

Center for the Evolutionary Origins of Human Behavior, Kyoto University○

Ken-ichi Nishijima

○

○

○

○

○

Masaki Isoda○○

Avian Bioscience Research Center, Graduate School of Bioagricultural Sciences, Nagoya
University, Tokai National Higher Education and Research System

Takashi Kato

Faculty of Education and Integrated Arts and Sciences, Waseda University

Haruki Ochi

Amphibian Research Center, Hiroshima University

Institute for Promotion of Medical Science Research, School of Medicine, Yamagata University

Hitoshi Okamoto

RIKEN Center for Brain Science

Shin-ichi Higashijima

National Institute for Basic Biology, National Institutes of Natural Sciences

Masaru Matsuda

Center for Bioscience Research and Education, Utsunomiya University

Masatsugu Hashiguchi

Faculty of Regional Innovation, University of Miyazaki

Yutaka Satou

Graduate School of Science, Kyoto University

Koichi Kawakami
Kiyoshi Naruse

Shigehiro Kuraku

Yasunori Sasakura

Department of Gene Function and Phenomics, National Institute of Genetics, Research
Organization of Information and Systems
Lab of Bioresources, National Institute for Basic Biology, National Institutes of Natural Sciences
Department of Genomics and Evolutionary Biology, National Institute of Genetics,
Research Organization of Information and Systems
Shimoda Marine Research Center, University of Tsukuba

Manabu Yoshida

Misaki Marine Biological Station, Graduate School of Science, The University of Tokyo

Toshiyuki Takano

Applied Biology, Kyoto Institute of Technology

Tsuguru Fujii

Institute of Genetic Resources, Graduate School of Bioresources and Bioenvironmental

Zenta Kajiura

Faculty of Textile Science and Technology, Shinshu University

Kuniaki Saito

Takeshi Awasaki

Yutaka Sato○

National Institute for Physiological Sciences, National Institutes of Natural Sciences

Graduate School of Integrated Sciences for Life, Hiroshima University

Hajime Ogino○○

Experimental Animal Division, RIKEN BioResource Research Center

Hiroyuki Horiuchi

Shohei Mitani○

Lotus japonicus & Glycine
max

Chrysanthemums

Atsushi Yoshiki○○

Tomatoes

Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

Katsuki Nakamura

B

Wheat

BarleyDepartment of Animal Experimentation, Foundation for Biomedical Research andInnovation at Kobe○

B

Silkworms

Experimental Animal Division, RIKEN BioResource Research Center

The Institute of Medical Science, The University of Tokyo

MedakaDrosophila

Shigeharu Wakana

Organization nameTomoji Mashimo○

Ciona intestinalis

Principal

Investigator

Department of Chromosome Science, National Institute of Genetics, Research
Organization of Information and Systems
School of Medicine, Kyorin University

Science, Kyushu University

Tokyo Women’ s Medical University School of Medicine

Masatomo Kobayashi

Experimental Plant Division, RIKEN BioResource Research Center

Toshihiro Kumamaru

Institute of Genetic Resources, Faculty of Agriculture, Kyushu University

Kazuhiro Sato

Institute of Plant Science and Resources, Okayama University

Ryohei Terauchi

Department of Genomics and Evolutionary Biology, National Institute of Genetics,
Research Organization of Information and Systems
Graduate School of Agriculture, Kyoto University

Masatsugu Hashiguchi

Faculty of Regional Innovation, University of Miyazaki

Tohru Ariizumi

Institute of Life and Environmental Sciences, University of Tsukuba

Shusei Sato

Kentaro Yano

Makoto Kusaba

Michiharu Nakano

Graduate School of Life Sciences, Tohoku University
School of Agriculture, Meiji University

Graduate School of Integrated Sciences for Life, Hiroshima University

Research and Education Faculty, Natural Sciences Cluster, Agriculture Unit, Kochi University

45

Bioresource category
Morning glories
Algae

＊
○

Eiji Nitasaka○

Masanobu Kawachi

B

Paramecium○

Mushrooms○

Cellular slime molds

General microbes

Pathogenic bacteria○

Takashi Yaguchi

Human and animal cells
DNA-related materials＊ ○ : Core Center

Osamu Kaneko

Institute of Tropical Medicine (NEKKEN), Nagasaki University
Research Institute for Microbial Diseases, Osaka University

Haruyoshi Tomita

Graduate School of Medicine, Gunma University

Keita Matsuno

Research Center for Zoonosis Control, Hokkaido University

National Research Center for the Control and Prevention of Infectious Diseases, Nagasaki
University

Yasushi Kawaguchi

Institute of Medical Science, The University of Tokyo

Yoshihiro Miwa

Gene Engineering Division, RIKEN BioResource Research Center

Tokiko Nagamura-Inoue
Yukio Nakamura

Yukio Nakamura○

Medical Mycology Research Center, Chiba University

Center for Conservation of Microbial Genetic Resource, Institute for Advanced Study, Gifu
University, Tokai National Higher Education and Research System

Jiro Yasuda○

Department of Gene Function and Phenomics, National Institute of Genetics, Research
Organization of Information and Systems

Graduate School of Pharmaceutical Sciences, Kyushu University

Tetsuya Iida○

Faculty of Life Sciences, Hiroshima Institute of Technology

Tsutomu Katayama

Takeshi Kobayashi

Human cord blood cells

Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University

Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center

Kaori Tanaka

Human pathogenic
viruses

Institute of Life and Environmental Sciences, University of Tsukuba

Natural Science Center for Basic Research and Development, Hiroshima University

Hironori Niki○

Joint Faculty of Veterinary Medicine, Yamaguchi University

Kenji Kitamura○

B

Faculty of Science, Hokkaido University

Graduate School of Science, Osaka Metropolitan University

Moriya Ohkuma○

Biodiversity Division, National Institute for Environmental Studies

Taro Nakamura

Minetaka Sugiyama

B

Pathogenic eukaryotic
microbes

Kobe University Research Center for Inland Seas

Tsuyoshi Ichiyanagi○

Prokaryotes(E.coli, B. subtilis )Shinya Uwai

Hidekazu Kuwayama

B

Graduate School of Science, Kyushu University

National Institute for Basic Biology, National Institutes of Natural Sciences

Masahisa Watarai○

Organization name

Atsushi Hoshino

Kazuhiro Kogame

○

Yeast

Principal

Investigator

Yoshihiro Miwa

None : Sub-Core Center

Research Institute for Microbial Diseases, Osaka University

Department of Cell Processing and Transfusion, IMUST Hospital, The Institute of Medical
Science, The University of Tokyo
Cell Engineering Division, RIKEN BioResource Research Center

Cell Engineering Division, RIKEN BioResource Research Center

Gene Engineering Division, RIKEN BioResource Research Center

B : Backup Facility (Sub-Core Center with backup purpose)

Information Center Program
Category＊○

Sub-category

Information Center

GAIN

JBIF

＊

46

Shoko Kawamoto

Satoshi Hirata

Utsugi Jinbo

Hiroya Yamano

Mutsuaki Suzuki

Information

Human resource
development forexternal verification ofanimal experiments

Person in Charge

ABS

Public relations○○ : Core Center None : Sub-Core Center

Katsuyuki Eguchi
Ayako Ishikawa

Organization name

Department of Informatics, National Institute of Genetics, Research Organizationof Information and Systems

Wildlife Research Center, Kyoto University

Center for Collections, National Museum of Nature and Science

Biodiversity Division, National Institute for Environmental Studies

ABS Support Team for Academia, NIG INNOVATION, National Institute of Genetics,
Research Organization of Information and Systems

Graduate School of Science, Tokyo Metropolitan University

Academic-Collaboration Risk Management Department, Academic Research& Industry-Academia-Government Collaboration Office, Tokai National Higher
Education and Research System

Mikihito Kanou

Mie Regional Plan Co-creation Organization, Mie University

Ayumi Koso

NBRP Public Relations Office, National Institute of Genetics, Research
Organization of Information and Systems

Chihiro Koshimoto

Japanese Association for Laboratory Animal Science

Hiroya Yamano

Biodiversity Division, National Institute for Environmental Studies

NBRP network of Japan (5th Phase)
1 Hokkaido University● Algae ● Human pathogenic viruses

3 Yamagata University● Clawed frogs & Newts

Resource Center Program: ● Core Center ● Sub-Core Center
Information Center Program: ● Core Center ● Sub-Core Center●Lotus japonicus & Glycine max

2 Tohoku University

4 Utsunomiya University

7 NIES

9 RIKEN CBS

12 Meiji University

13 The University of Tokyo

34 Osaka University● Mice ●Arabidopsis ● General microbes● Human and animal cells ● DNA-relatedmaterials ● Rats ● Human pathogenic viruses● Human cord blood cells● Aged mice
36 Foundation for Biomedical
Research and Innovation at Kobe

35 Kobe University● Mushrooms

39 Hiroshima University●Clawed frogs & Newts ●Chrysanthemums●Chickens & Quails ●Yeast

40 Hiroshima Institute of Technology ● Yeast●Caenorhabditis elegans
16 Tokyo Women’s Medical
University School of Medicine● Barley

41 Kochi University● Chrysanthemums

43 Kyushu University● Silkworms ● Morning glories ● Rice
● Prokaryotes (E. coli, B. subtilis )42 Yamaguchi University

44 Nagasaki University
45 University of Miyazaki● Human resource development for externalverification

18 National Museum of Natureand Science

37 Tottori University● Tomatoes●Drosophila

17 JALAS● Algae38 Okayama University● Clawed frogs & Newts

15 Kyorin University● Pathogenic bacteria● Human pathogenic viruses● Zebrafish● Human cord blood cells ● Rats
● Human pathogenic viruses

14 Waseda Univeristy●Drosophila

33 Osaka Metropolitan University ● Yeast

10 Tokyo Metropolitan University ● ABS
11 Gakushuin University● Rats ● Wheat ●Ciona intestinalis ● GAIN

● ABS

● Tomatoes ● Cellular slime molds● Algae ● ABS ● JBIF

8 RIKEN BRC

31 Kyoto University

32 Kyoto Institute of Technology● Pathogenic bacteria

6 University of Tsukuba● Pathogenic bacteria

30 Mie University● Medaka

5 Gunma University

29 Gifu University●Paramecium● Human pathogenic viruses● Pathogenic eukaryotic microbes●Lotus japonicus & Glycine max ● Medaka● JBIF

19 Chiba University

20 MMBS, The University of Tokyo● Pathogenic eukaryotic microbes●Ciona intestinalis

21 National Institute of Genetics ●Drosophila ● Rice
● Prokaryotes (E. coli , B. subtilis ) ● Zebrafish● Medaka ● Information center● ABS ● Public relations

22 SMRC, University of Tsukuba●Ciona intestinalis

24 National Institute for Basic
Biology● Medaka ● Morning glories ● Zebrafish

23 Shinshu University● Silkworms

25 National Institute forPhysiological Sciences● Japanese macaque

27 Nagoya University● Chickens & Quails

26 EHUB, Kyoto University

1● Japanese macaque

28 Tokai National Higher Education ● ABS
and Research System

3

5

37
42

40

38

39

43

23
29 26
32
27 24 25
35 33 31
36 34
30 28

2

4

6 7
9
8
10 11 12 13 14
15 16 17 18 19
21
20
22

41

44

45

47

Contact

NBRP Public Relations Office
National Institute of Genetics
1111 Yata, Mishima, Shizuoka 411-8540, Japan
Phone: +81-55-981-6876
E-mail: nbrp-pr@nig.ac.jp
URL: https://nbrp.jp/en/

2023.01

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