㻯㼀䛻䜘䜛⫵⅖ീ㻌⣽⬊䜢䝝䜲䝆䝱䝑䜽䛩䜛䜴䜲䝹䝇㻌㻌㻌㻌 㻌 䜴䜲䝹䝇䛾ᵓ㐀䝍䞁䝟䜽㉁䛾ື䛝䜢⏕䛝䛯⣽⬊䛷ほ䜛㻌㻌 㻌 㻌 㻌 㻌 㻌 㻌 㻌 㻌 㻌 䜴䜲䝹䝇䛳䛶䛛䛧䛣䛔䟿䟿㻌᪥ᮏ኱Ꮫ་Ꮫ㒊ᾘ໬ჾ⫢⮚ෆ⛉㻌 㻌 㻌 ◊✲ဨ㻌 㻌 㻌 Ⱚ⏣㻌 ᩄඞ21㻟㻰㻯㼀䛻䜘䜛デ᩿㻌43䜲䞁䝣䝹䜶䞁䝄䜴䜲䝹䝇䛾デ᩿ⓒ⪺䛿୍ぢ䛻ዴ䛛䛪㰯⭍䛛䜙䛾䝃䞁䝥䝹᥇ྲྀ↓䜲䞁䝣䝹䜶䞁䝄デ᩿䜻䝑䝖䛷䛾ุᐃ↓䝍䝭䝣䝹䞉䝸䝺䞁䝄➼䛾ᢠ䜲䞁䝣䝹䜶䞁䝄⸆䛾ᢞ୚䜲䞁䝣䝹䜶䞁䝄䜴䜲䝹䝇ឤᰁ䛜⣽⬊䛾୰䛷䚸䛹䛾䜘䛖䛻㐍⾜䛧䛶䛔䜛䛾䛛䜢┤᥋┠䛷ほᐹ䛧䛯䛔䚹䛣䛾ほᐹ䜢㏻䛧䛶䚸䜲䞁䝣䝹䜶䞁䝄䜴䜲䝹䝇ឤᰁ㐣⛬䛻㛵䛩䜛ᮍ▱䛾⌧㇟䜢ぢ䛴䛡䜙䜜䛺䛔䛛䠛

56䛣䜜䜎䛷䛻⾜䜟䜜䛶䛝䛯⏕⣽⬊䜢⏝䛔䛯䜴䜲䝹䝇ឤᰁ䛾ほᐹ᪉ἲ䜴䜲䝹䝇䛾┤᥋䝷䝧䝹䛹䛾䜘䛖䛻䛧䛯䜙䜲䞁䝣䝹䜶䞁䝄䜴䜲䝹䝇ឤᰁ㐣⛬䜢⌧ᅾ㐍⾜ᙧ䛷ほ䜛䛣䛸䛜䛷䛝䜛䛾䛛䠛䜴䜲䝹䝇䝀䝜䝮䜴䜲䝹䝇䡉RNA䛾ᶆ㆑⺯ගᶆ㆑ᢠయ䛾㻌⣽⬊ෆ䜈䛾ᑟධ㻌ὀ䠅⺯ග䝍䞁䝟䜽㉁䜢㝖䛟

87⺯ග䝍䞁䝟䜽㉁㻌

䜸䝽䞁䜽䝷䝀䠎䠌䠌䠔ᖺ䝜䞊䝧䝹໬Ꮫ㈹㻌
⥳Ⰽ⺯ග䝍䞁䝟䜽㉁䠄㻳㻲㻼㻕䛾Ⓨぢ䛸䛭䛾ᛂ⏝㻌䜸䝽䞁䜽䝷䝀䠄ᚚ᳐Ỉẕ䚸ᚚ᳐ᾏ᭶䠅䛿䚸䝠䝗䝻⹸⥘䛻ᒓ䛩䜛↓⬨᳝ື≀䚹᪥ᮏྛᆅ䛾ἢᓊ䛷ぢ䜙䜜䜛䚹ച䛿☇䜢㏫䛥䛻䛧䛯䜘䛖䛺ᙧ䜢䛧䛶䛚䜚䚸㏱᫂䛷ෆഃ䛾ᨺᑕ⟶䛜䛿䛳䛝䜚䛸እഃ䛛䜙ぢ䛘䜛䚹᭱኱ചᚄ䛿20cm䛻䛚䜘䜃䚸䝠䝗䝻⹸⥘䛷᭱኱䚹㻝㻥㻥㻞ᖺ䜸䝽䞁䜽䝷䝀䛛䜙㻳㻲㻼㑇ఏᏊ䛜䜽䝻䞊䝙䞁䜾䛥䜜䜛䚹䛣䛾㻳㻲㻼㑇ఏᏊ䜢⥺⹸䛻Ⓨ⌧䛥䛫䜛䛣䛸䛻ᡂຌ㻌ୗᮧ㻌 ⬶㻌 ༤ኈ㻌㻹㼍㼞㼠㼕㼚㻌㻯㼔㼍㼘㼒㼕㼑㻌 ༤ኈ㻌㻾㼛㼓㼑㼞㻌㼅㻌㼀㼟㼕㼑㼚㻌 ༤ኈ㻌109ᑠ⏣ᛴ᪂Ụ䝜ᓥỈ᪘㤋⺯ග䝍䞁䝟䜽㉁㻌
ECFP
Aeqorea GFP䜸䝽䞁䜽䝷䝀EYFP
EGFP

mOrange
Ds RedmRFPmCherry㻌䜽䝷䝀䝣䜯䞁䝍䝆䞊䝽䞊䝹䝗㻌㼕㼚㻌᪂Ụ䝜ᓥỈ᪘㤋㻞㻜㻝㻟㻌㻌㛤ദᮇ㛫㻌䠖㻌㻞㻜㻝㻟ᖺ㻣᭶㻝᪥䠄᭶䠅䡚㻤᭶㻟㻝᪥䠄ᅵ䠅㻌mRasberry
䝃䞁䝂

mPlum

1112䜲䞁䝣䝹䜶䞁䝄䜴䜲䝹䝇㻌ศ⠇㻝㻌ศ⠇㻞㻌㻌㻌㻌㻌ศ⠇㻟㻌㻌㻌㻌㻌㻌㻌ศ⠇㻠㻌ศ⠇㻡㻌ศ⠇㻢㻌ศ⠇㻣㻌㻌㻌ศ⠇㻤㻌㻌㻌⺯ග䝍䞁䝟䜽㉁䜢䜲䞁䝣䝹䜶䞁䝄䜴䜲䝹䝇䛾䝍
䞁䝟䜽㉁䛸⼥ྜ䛥䛫䜛䛸䚸⡆༢䛻ឤᰁ㐣⛬䜢ほ䜛䛣䛸䛜䛷䛝䛺䛔䛛䠛㻌䠍⢏Ꮚᙜ䛯䜚䛾㻌䝍䞁䝟䜽㉁ᩘ㻌
PB2㻟㻜㻙㻢㻜㻌
PB1㻟㻜㻙㻢㻜㻌
PB1-F2㻙㻌PB1-N40
㻫㻌
PA㻟㻜㻙㻢㻜㻌
PA-X
㻫㻌
PA-N155
㻫㻌PA-N182㻫㻌
HA㻡㻜㻜㻌
NP㻝㻜㻜㻜㻌
NA㻝㻜㻜㻌
M1
㻟㻜㻜㻜㻌
M2
㻞㻜㻙㻢㻜㻌
NS1
㻙㻌
NS2
㻝㻟㻜㻙㻞㻜㻜㻌

1413

㻹㻝䝍䞁䝟䜽㉁䛾ᙺ๭㻌㻳㻲㻼㻛㻹㻝䛸㻹㻝㻛㻳㻲㻼䛾Ⓨ⌧㻌㻳㻲㻼㻛㻹㻝㻌

㻹㻝㻛㻳㻲㻼㻌

䜴䜲䝹䝇⢏Ꮚ㻌䜴䜲䝹䝇⭷䛾⿬ᡴ䛱䛩䜛㻌䛣䛸䛻䜘䜛⢏Ꮚᵓ㐀䛾⥔ᣢ㻌㻌ឤᰁ⣽⬊㻌᰾ෆ䛷䝀䝜䝮」〇䛾ㄪ⠇㻌䜴䜲䝹䝇䝀䝜䝮䛾᰾እ㍺㏦㻌
ฟⱆ㻌

1516

䜴䜲䝹䝇ឤᰁGFP/M1ᐃᖖⓎ⌧⣽⬊䛾䝷䜲䝤䜲䝯䞊䝆䞁䜾䛣䜜䜎䛷䚸䜲䞁䝣䝹䜶䞁䝄䜴䜲䝹䝇ឤᰁ⣽⬊䜢ᢠ
M1ᢠయ䛷ᰁⰍ䛧䛶䜒䚸䜴䜲䝹䝇ឤᰁMDCKGFP/M1䛷ほᐹ䛥䜜䛯䜘䛖䛺䚸᰾ෆ䛷M1䛾㢛⢏≧䛾㞟✚䛿ほᐹ䛥䜜䛺䛛䛳䛯䚹䛺䛬䚸௒䜎䛷Ⓨぢ䛥䜜䛶䛔䛺䛛䛳䛯䛾䠛

1718ឤᰁ⣽⬊ෆ䛾䝍䞁䝟䜽㉁䜢⺯ග㢧ᚤ㙾䛷㻌ほᐹ䛩䜛䛻䛿㻌ᢠ㻳㻲㻼ཬ䜃ᢠ㻹㻝ᢠయ䛻䜘䜛ච␿ᰁⰍ䛷䛿᰾ෆ䛻㢛⢏䛿ほᐹ䛥䜜䛺䛔㻌β၃௨ᑥඥửဇẟẺẆᖩήβ၃௨ᑥඥửᘍạ࣏ᙲầẝỦᴾ

ᵥᵤᵮᵍᵫᵏᴾỸỶἽἋज़௨ኬᏘửᴾἣἻἭἽἲỴἽἙἤἛሁỂ‫ܭ׍‬ᴾḶᴾᵲᶐᶇᶒᶍᶌᴾᵶᵏᵎᵎỂኬᏘᐏỆᆭửẝẬỦᴾḶᴾᾀഏ৴˳Ể௨ᑥᴾ
Ḷᴾᾁഏ৴˳ίᖩή೅ᜤὸỂ௨ᑥᴾ
Ḷᴾᖩή᫋ࣇᦟỂỉᚇ‫ݑ‬ᴾᵟᶌᶒᶇᵋᵫᵏᴾ

ᵟᶌᶒᶇᵋᵥᵤᵮᴾ৴˳Ị᫄ቩ࢟঺ẲềẟỦ‫ئ‬৑ỆỊỴἁἍἋỂẨễẟỉ
ỂỊễẟẦᾎᾎᴾ

19

20

ᵥᵤᵮửဇẟẺᚐௌỆợụẆỶὅἧἽỺὅἈỸỶἽἋᵫᵏἑὅἣἁឋầఋϋỂ᫄ቩನᡯửᅆẴểẟạૼẲẟྵᝋửႆᙸỂẨẺẇᴾ

21

22
ᵫᵏἑὅἣἁឋҥ཯ႆྵኬᏘỂờᵫᵏἑὅἣἁឋỊఋϋỂ᫄ቩ҄ẴỦᴾ

ᵥᵤᵮᵍᵫᵏửဇẟẺᚐௌỉբ᫆ໜᴾ

ᵣᵥᵤᵮᴾ

23

৴ᵫᵏ৴˳ὺᵟᶊᶃᶖᵿᵒᵖᵖᴾ

ᵥᵤᵮᵍᵫᵏႆྵኬᏘᴾ• Ў‫܇‬᣽ầᵐᵕᾺᵢỂẝỦᵥᵤᵮửᵫᵏἑὅἣἁឋίᾁᾅ
ᾺᵢᵇỆ˄ьẲẺᵥᵤᵮᵍᵫᵏỆấẟềẆẸỉਫѣỊΨỉᵫᵏἑὅἣἁឋỉẸủử‫ܦ‬μỆӒପẴỦẦᴾᴾ• ᵫᵏἑὅἣἁឋỊࢸ஖ἑὅἣἁឋỂẝỦầẆᵥᵤᵮᵍᵫᵏỊỸỶἽἋज़௨ЭẦỤ‫נ܍‬ẴỦẮểỆễỦᴾᴾᵫᵏᴾႆྵኬᏘᴾ

24

ᵣᵥᵤᵮᵍᵫᵏỂỊᵫᵏỉೞᏡửμềửᚇ‫ݑ‬ỂẨễẟᴾ৴ᵫᵏ৴˳ᴾ

ᾜᾀႆྵኬᏘᴾỶὅἧἽỺὅἈỸỶἽἋỉᇹᾆЎራᴾỆᵥᵤᵮᢡˡ‫܇‬ử਴λẴỦẮểỊЈஹễẟẇᴾᴾᴾ˴ẦᵥᵤᵮỆˊỪỦẆဃኬᏘᚇ‫૾ݑ‬ඥỊễẟẦᾎᴾ

ᵣᵥᵤᵮᵍᵫᵏᵉᵫᵏᴾσႆྵኬᏘᴾ

ᵣᵥᵤᵮᴾ

ᵣᵥᵤᵮᴾ

ᵣᵥᵤᵮᵍᵫᵏᴾ
ႆྵኬᏘᴾ

25

26
Tetra-Cysteine (TC ἑἂ)ỉ౨ЈҾྸᵲᶃᶒᶐᵿᵋᵡᶗᶑᶒᶃᶇᶌᴾ ἑἂᴾ ᵆᵲᵡᴾἑἂὸỆợỦἻỶἨỶἳὊἊὅἂᴾTC 䝍䜾ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᵱᶁᶇᶃᶌᶁᶃᵌᴾᵏᵗᵗᵖᴾᵨᶓᶊᴾᵏᵎᵙᵐᵖᵏᵆᵓᵑᵕᵒᵇᵘᵐᵔᵗᵋᵕᵐᵌᴾ

M1䝍䞁䝟䜽㉁㻌

ᵲᵡᴾἑἂỉМໜᴾЎ‫܇‬᣽ầᵎᵌᵖᶉᵢể‫ݱ‬Ằẟẟᴾᵋᵡᶗᶑᵋᵡᶗᶑᵋᵮᶐᶍᵋᵥᶊᶗᵋᵡᶗᶑᵋᵡᶗᶑᴾ

ᵱᶎᶃᶁᶇᶄᶇᶁᴾᶁᶍᶔᵿᶊᶃᶌᶒᴾᶊᵿᶀᶃᶊᶇᶌᶅᴾᶍᶄᴾᶐᶃᶁᶍᶋᶀᶇᶌᵿᶌᶒᴾᶎᶐᶍᶒᶃᶇᶌᴾᶋᶍᶊᶃᶁᶓᶊᶃᶑᴾᶇᶌᶑᶇᶂᶃᴾᶊᶇᶔᶃᴾᶁᶃᶊᶊᶑᵌᴾᵥᶐᶇᶄᶄᶇᶌᴾᵠᵟᵊᴾᵟᶂᵿᶋᶑᴾᵱᵰᵊᴾᵲᶑᶇᶃᶌᴾᵰᵷᵌᴾᵌᴾᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᴾ ᵱᶁᶇᶃᶌᶁᶃᵌᴾᵐᵎᵎᵐᴾᵟᶎᶐᴾᵏᵗᵙᵐᵗᵔᵆᵓᵓᵔᵕᵇᵘᵓᵎᵑᵋᵕᵌᴾ

㻲㼘㻭㼟㻴㻌ヨ⸆㻌㻔㠀⺯ග㻕㻌ᵫᶓᶊᶒᶇᶁᶍᶊᶍᶐᴾᵿᶌᶂᴾᶃᶊᶃᶁᶒᶐᶍᶌᴾᶋᶇᶁᶐᶍᶑᶁᶍᶎᶇᶁᴾᶇᶋᵿᶅᶇᶌᶅᴾᶍᶄᴾᶁᶍᶌᶌᶃᶖᶇᶌᴾᶒᶐᵿᶄᶄᶇᶁᶉᶇᶌᶅᵌᴾᵥᵿᶇᶃᶒᶒᵿᴾᵥᵊᴾᵢᶃᶃᶐᶇᶌᶁᶉᴾᵲᵨᵊᴾᵟᶂᵿᶋᶑᴾᵱᵰᵊᴾᵠᶍᶓᶕᶃᶐᴾᵨᵊᴾᵲᶍᶓᶐᴾᵭᵊᴾᵪᵿᶇᶐᶂᴾᵢᵵᵊᴾᴾᵱᶍᶑᶇᶌᶑᶉᶗᴾᵥᵣᵊᴾᵲᶑᶇᶃᶌᴾᵰᵷᵊᴾᵣᶊᶊᶇᶑᶋᵿᶌᴾᵫᵦᵌᴾ㻲㼘㻭㼟㻴㻌ヨ⸆㻌㻔⺯ග㻕㻌

FlAsHヨ⸆䛾฼Ⅼ⏕⣽⬊䛾⭷䜢㏱㐣䛩䜛䛣䛸䛜䛷䛝䜛
FlAsH䛸ReAsH䛾䠎Ⰽ䜢౑䛔䜟䛡䜛䛣䛸䛜䛷䛝䜛䝟䝹䝇䞊䝏䜵䞊䝇ᐇ㦂䜢⾜䛖䛣䛸䛜䛷䛝䜛27
ᵲᵡᵍᵫᵏỸỶἽἋỉ˺ᙌᴾ28㼀㻯㻛㻹㻝䜴䜲䝹䝇ឤᰁ⣽⬊䛻䛚䛡䜛㻌㻲㼘㻭㼟㻴ᰁⰍ䛾≉␗ᛶ㻌

Wild TypemRNA
cga ttc aag --- --- --- --- --- --- tga tcc tc
M1 Protein R F K--- --- --- --- --- --- *M2 Protein
D S S--- --- --- --- --- --- D P

Dic

FlAsH

TC tag Segment 7 (M1/TC䜴
䜴䜲䝹䝇䠅

PFU/ml (log10)

mRNAcga ttc aag TGC TGC CCT GGC TGC TGC tga tcc
M1 Protein R F K C C P
G C C * TC Tag
M2 Protein
D S S A AL AA A D P

TC/M1

Wild
M1/TC

Time after infection (h)

Wild

2930

TC/M1䜴䜴䜲䝹䝇ឤᰁ⣽⬊䛻䛚䛡䜛FlAsHീ䛾⤒᫬ኚ໬ኬᏘឋỆ‫נ܍‬ẴỦᵫᵏᵍᵲᵡἑὅἣἁឋỉᨼᆢನᡯỊ˴Ầᵝᴾ3231
ज़௨ኬᏘỂỉᵫᵏᵍᵲᵡἑὅἣἁឋỉή‫ܖ‬ႎЏ༾΂ᴾ⣽⬊㉁䛾㼀㻯㻛㻹㻝㢛⢏ᵓ㐀䛿⣽⬊⾲㠃䛾㻴㻭䝍䞁䝟䜽㉁䛸୍⮴䛩䜛㻌ᵲᶍᶎᴾᵟᶌᶒᶇᵋᵦᵟᴾᵤᶊᵟᶑᵦᴾᵫᶃᶐᶅᶃᴾኬᏘɥᢿᴾἋἻỶἋᴾɶ࣎ᴾἋἻỶἋᴾᵠᶍᶒᶒᶍᶋᴾ3334

ኬᏘᘙ᩿ỂỉHAểM1ἑὅἣἁỉ‫נޅ‬
DAPI

anti-HA

anti-M1

MergeኬᏘᘙ᩿ỂỉHAểM1ἑὅἣἁỉ‫נޅ‬⣽⬊ୖ㒊䝇䝷䜲䝇

DAPI

anti-HA

anti-M1

MergeἣἻἭἽἲỴἽἙἤἛ‫ܭ׍‬ẲẺኬᏘỂỊẆHAểM1ầኬᏘᘙ᩿Ểσ‫נޅ‬ẴỦ΂Ịᚇ‫ݑ‬Ằủễẟ୰ᚰ㒊
䝇䝷䜲䝇

35

36

䜴䜲䝹䝇⢏Ꮚ䛾㻲㼘㻭㼟㻴䝷䝧䝹㻌㼀㼑㼠㼞㼍㻙㻯㼥㼟䝍䜾᳨ฟ⣔䜢⏝䛔䜜䜀䚸⏕⣽⬊䛰䛡䛷䛺䛟䚸Ⰽ䚻䛺䜰䝥䝸䜿䞊䝅䝵䞁䜢฼⏝䛧䛶ゎᯒ䛜䛷䛝䜛䚹㻌37

38㻹㻝㻛㼀㻯䝍䞁䝟䜽㉁䛾≉␗ⓗ᳨ฟ㻌㻿㻰㻿㻙㻼㻭㻳㻱㻌㼃㼕㼘㼐㻌 㻹㻝㻛㼀㻯㻌㼃㼑㼟㼠㼑㼞㼚㻌㼎㼘㼛㼠㼠㼕㼚㼓㻌㼃㼕㼘㼐㻌 㻹㻝㻛㼀㻯㻌㼃㼕㼘㼐㻌 㻹㻝㻛㼀㻯㻌㼃㼕㼘㼐㻌 㻹㻝㻛㼀㻯㻌㼃㼕㼘㼐㻌 㻹㻝㻛㼀㻯㻌䝞䝷Ⰽ䛷䛿䛺䛔㼀㼑㼠㼞㼍㻙㻯㼥㼟㼠㼑㼕㼚㻌䝍䜾᳨ฟ⣔㻌㼀㼛㼠㼍㼘㻌㻼㼞㼛㼠㼑㼕㼚㻌㻭㼚㼠㼕㻙㻹㻝㻌㻲㼘㻭㼟㻴㻌㻲㼘㻭㼟㻴㻌㻹㼑㼞㼓㼑㻌

3940

㼀㻯㻛㻹㻝௨እ䛾⤌᥮䛘య䜴䜲䝹䝇䛿స〇䛷䛝䛺䛔䛛䠛㻌ᵲᵡᵍᵫᵏỸỶἽἋᴾᵲᵡᵍᵬᵮỸỶἽἋᴾ㻌 䜴䜲䝹䝇ឤᰁ㻹㻰㻯㻷㻙㻳㻲㻼㻛㻹㻝⣽⬊䜔㻹㻝㻛㼀㻯䜴䜲䝹䝇ឤᰁ⣽⬊䛷ほᐹ䛥䜜䜛᰾ෆ䛾㢛⢏䛿䛺䜣䛰䜝䛖䠛㻌ᵲᵡᵍᵫᵐỸỶἽἋᴾᵲᵡᵍᵦᵟỸỶἽἋᴾ᣼ဃ‫׹‬ᴾ4142

䜴䜲䝹䝇ឤᰁMDCK-GFP/M1⣽⬊䛷ほᐹ䛥䜜䜛᰾ෆ㢛⢏䛿PML䛸୍⮴䛩䜛⣽⬊䛾᰾ෆ䛾ᵓ㐀㻌㻰㻭㻼㻵䛻䜘䜛㻌᰾ᰁⰍ㻌ᵮᵫᵪᴾᵥᵤᵮᵍᵫᵏᴾ

ᵫᶃᶐᶅᶃᴾᵱᵡᵑᵓᴾ㻰㻭㻼㻵㻗఩┦ᕪ㻌Dojin News䜘䜚ᘬ⏝4443

ᵲᵡᵍᵫᵏỸỶἽἋज़௨ኬᏘỂᚇ‫ݑ‬ẰủỦ᫄ቩನᡯờᵮᵫᵪểɟᐲẴỦᴾᵢᵧᵡᴾᵤᶊᵟᶑᵦᴾᶎᶋᵡᶆᶃᶐᶐᶗᵋᵮᵫᵪᴾ

ᵫᶃᶐᶅᶃᴾ䜲䞁䝣䝹䜶䞁䝄䜴䜲䝹䝇㻹㻝䝍䞁䝟䜽㉁䛜᰾ෆ䛾㻼㻹㻸䝪䝕䜱䞊䛻㞟✚䛩䜛䛾䛿䛺䛬䠛㻌

4546

㻼㻹㻸䝪䝕䜱䞊䛸䛿䠛㻌㻌 ⒴ᢚไ㑇ఏᏊ⏘≀䛾㻼㻹㻸䠄㻼㼞㼛㼙㼥㼑㼘㼛㼏㼥㼠㼕㼏㻌㼘㼑㼡㼗㼑㼙㼕㼍㻕䝍䞁䝟䜽㉁䜢୺せ䛺ᵓᡂ䝍䞁䝟䜽㉁䛩䜛᰾ෆᵓ㐀≀䛷䛒䜛䚹⣽⬊䛷䛿㻜㻚㻞䡚䠍㼡㼙⛬ᗘ䛾⌫≧䛾ᵓ㐀య䛜㻝㻜䡚㻟㻜ಶ⛬ᗘほᐹ䛥䜜䜛䚹㻌㻌 䛣䛾㻼㻹㻸䝪䝕䜱䞊䛿⣽⬊䛾⪁໬䚸Ⓨ⒴䚸䜴䜲䝹䝇ឤᰁ䚸䜰䝫䝖䞊䝅䝇䜔㌿෗➼ᵝ䚻䛺㐣⛬䛻㛵୚䛩䜛䚹㻌ᵮᵫᵪἮἙỵὊỉɼᙲನ঺঺ЎỂẝỦẆᵮᵫᵪỊỶὅἧἽỺὅἈỸỶἽἋỉ‫ف‬഻ử৮СẴỦẮểầ‫إ‬ԓẰủềẟỦᴾ䜰䝫䝖䞊䝅䝇㑇ఏᏊⓎ⌧ไᚚ4748

ᵫᶇᶌᶇᵋᵰᶃᶎᶊᶇᶁᶍᶌᴾᵱᶗᶑᶒᶃᶋửဇẟẺᵮᵫᵪỆợỦỸỶἽἋἯἼἳἻὊἎ෇ࣱỉ৮СᴾỸỶἽἋἯἼἳἻὊἎ෇ࣱᴾᵮᵫᵪỊ‫ٶ‬ૠỉᵴᵿᶐᶇᵿᶌᶒầ‫נ܍‬ẴỦᴾMarie-Claude Geoffroy and Mounira K. Chelbi-Alix
JOURNAL OF INTERFERON & CYTOKINE RESEARCH
Volume 31, Number 1, 2011㻌 䜘䜚ᘬ⏝

140
120
100
80
60
40

20
049

50
M1Ⓨ
Ⓨ⌧⣽⬊䛷䛿䚸PML䛾ື䛝䛜ᢚไ䛥䜜䜛ᵥᵤᵮὉᵮᵫᵪỉᵤᵰᵟᵮᚐௌᴾ

100ᵤᵰᵟᵮᚐௌỉҾྸᴾ90ᵮᵫᵪỉᵤᵰᵟᵮᚐௌᴾ

80

ᖩήࢍࡇᴾ

70

PML2
ᵫᵏᵆᵋᵇᴾ
PML3
PML-M1ᵫᵏᵆᵉᵇᴾ
PML-M1

60
50
40
30
20
10
00200

400

600

800

1000

1200

଺᧓ᴾᴾᵆᶑᶃᶁᵇᴾ
Hela⣽⬊䛻GFP-PML䛚䜘䜃M1䜢Ⓨ⌧䛥䛫䚸GFP-PML䛾ືែ䜢ゎᯒ䛧䛯䚹M1䝍䞁䝟䜽㉁䛾Ⓨ⌧䛿ReAsHᰁⰍ䛻䜘䜚☜ㄆ䛧䛯䚹5251㻌 ⺯ග䝍䞁䝟䜽㉁䛷䛒䜛㻳㻲㻼䜒䛧䛟䛿㼀㻯䝍䜾䜢⏝䛔䛶䚸䜲䞁䝣䝹䜶䞁䝄䜴䜲䝹䝇㻹㻝䝍䞁䝟䜽㉁䜢⏕⣽⬊䛷ほᐹ䛩䜛䛣䛸䛻ᡂຌ䛧䛯䚹ほᐹ㐣⛬䛷㻹㻝䝍䞁䝟䜽㉁䛜᰾ෆᵓ㐀≀䛷䛒䜛㻼㻹㻸䝪䝕䜱䞊䛻㞟✚䛩䜛䛣䛸䜢᪂つ䛻Ⓨぢ䛧䛯䚹㻌㻌 㻹㻝䝍䞁䝟䜽㉁䛿㻼㻹㻸䝪䝕䜱䞊䜢䝝䜲䝆䝱䝑䜽䛩䜛䛣䛸䛻䜘䜚䚸㻼㻹㻸䛾ᢠ䜴䜲䝹䝇స⏝䜢ᢚไ䛧䛶䛔䜛ྍ⬟ᛶ䛜♧၀䛥䜜䛯䚹㻌㻹㻝䝍䞁䝟䜽㉁䛜᰾ෆ㢛⢏ᵓ㐀≀䛷䛒䜛㻼㻹㻸䝪䝕䜱䞊䛻㞟✚䛩䜛䛣䛸䛻䜘䜚䚸㻼㻹㻸䝪䝕䜱䞊䛾୺せᵓᡂᡂศ䛷䛒䜛㻼㻹㻸䛾ᢠ䜴䜲䝹䝇ຠᯝ䜢ᢚไ䛧䛶䛔䜛䛸⪃䛘䜙䜜䜛䚹㻌5354

ㅰ㎡

ଐஜ‫ܖٻ‬Ҕ‫ܖ‬ᢿᴾ ࣇဃཋ‫ܖ‬Ў᣼ᴾ
᱅ဋᴾ ԧᢊᴾ έဃᴾଔ߷ᴾ ୓ᴾ ᴾ ᴾ ૙੉ᴾிʮ‫ܖٻ‬Ҕᅹ‫ܖ‬ᄂᆮ৑ᴾඕ‫ޢ‬ᴾ ፯ᘽᴾ ૙੉ᴾଐஜ‫ܖٻ‬Ҕ‫ܖ‬ᢿᴾ ෞ֥҄Ꮑᐥϋᅹᴾౕ‫ޛ‬ᴾ ήࢠᴾ ૙੉ᴾဋ஭ᴾ ࢧ૙ᴾ έဃᴾ˙ᕲᴾ ๹ᴾ ᴾ έဃᴾ‫ܖٻ࢟ޛ‬ᴾ Ҕ‫ܖ‬ᢿᴾ ࣇဃཋ‫ܖ‬૙ܴᴾஜᣂᴾ ᛗ඙ᴾ ૙੉Ẇᴾ ᓌҾᴾ ѥनᴾ έဃᴾଐஜ‫ܖٻ‬Ҕ‫ܖ‬ᢿᴾ ငۡʴᅹ‫ܖ‬Ў᣼ᴾ‫ޛ‬ஜᴾ ೔ဃᴾ ૙੉ᴾᵬᵿᶒᶇᶍᶌᵿᶊᴾᵧᶌᶑᶒᶇᶒᶓᶒᶃᴾᶍᶄᴾᵟᶊᶊᶃᶐᶅᶗᴾᵿᶌᶂᴾᵢᶇᶑᶃᵿᶑᶃᴾᵆᵬᵧᵟᵧᵢᵇᴾᵨᶆᶍᶌᵿᶒᶆᵿᶌᴾᵵᴾᵷᶃᶕᶂᶃᶊᶊᴾᴾ έဃᴾἰỸὅἚἇỶἜỶҔᅹ‫ܖٻ‬ᴾ ࣇဃཋ‫ܖ‬૙ܴᴾɤ௎ᴾ ദ೔ᴾ έဃᴾ঺ᏋҔၲᄂᆮἍὅἑὊᴾ ငۡʴᅹᴾ‫ק‬ဋᴾ ᰺ྸ‫܇‬ᴾ έဃᴾ
ᑮဋᴾ औᴾ ᴾ ᴾ ᴾ έဃᴾ

55

56←上司の黒田先生ẝụầểạắằẟộẲẺᴾ←娘Ⱚ⏣㻌 ᩄඞ5758

䝯䝍䝜䞊䝹ᅛᐃ䛧䛯PR8ឤᰁMDCK⣽⬊䛻䛚䛡䜛
M1㢛⢏ᵓ㐀䛾⤒᫬ⓗኚ໬4䡄
䡄6䡄

12䡄

8䡄

Mock 12䡄