Cell Capture and Recovery

Add new depth torare cell studies with theGenesis System andCelselect Slides

TMCapture more.
Recover more.
Accomplish more.

New Depth to Rare Cell Research
Genesis CellIsolation Systemwith Celselect Slides• Simplified benchtop solution for rare cell isolation• High efficiency and gentle size-based capture• Customization for diverse applications anddifferent samples

Celselect Slides Enrichment Kit 2.0

Celselect Slides Enumeration Kits 2.0

Kit for cell capture and recovery
for downstream applications

Kits for cell capture, cell staining via
immunolabeling, and on-slide imaging

Cell Capture and Recovery

2

The Genesis System has two validated workflows:• Recovery of viable cells for downstream analysis• Capture and quantification of cells using microscopy

Sample Collection
■
■
■

Blood
Pleural fluid
Urine

Cell Capture■■

Sterilize and prime slide
Automated cell capture ofup to 10 ml of sample■

Either recover captured cells
for downstream analysis or
stain cells in the slide for
imaging and enumeration

Enrichment
Workflow

Enumeration
Workflow

Recovery for Downstream Analysis■

Staining and Imaging■

 ells can be characterized with methods such asC
sequencing (bulk or single-cell), digital PCR, FISH,
flow cytometry, or culturing cells for further in vitro
and in vivo analysis

■■

3DAPI

CD45

PanCKPD-L1

Label cells for downstream imaging
Image Celselect Slides with a fluorescence microscope
Import files from microscope to Rare Cell Analysis
Software, confirm cells of interest, and generate reportCapture More. Recover More.
Cell Capture Simplified with theGenesis System and Celselect Slides
Using patented microfluidic technology, the Genesis System and Celselect Slides enable:• Gentle capture of individual cells and cell clusters 8–30 μm, regardless of phenotype (Figures 1 and 3)• High cell capture rate and reliable detection of samples containing low cell concentrations (Figure 2)• Capture without Ficoll gradients, or RBC and WBC lysis preprocessing

1,200
Blood Cells

Cancer Cells

2015

Slide Pore Size

Cell size, μm

25Number of cells captured per ml

30105Ovary600
400200y = 0.9056(x) – 2.133
00Fig. 1. Pore size of Celselect Slides relative torange of blood and cancer cells. Various cell types
are suitable for capture. CNS, central nervous system;
CTC, circulating tumor cell; RBC, red blood cell;WBC, white blood cell.

200400600800
Number of cells spiked per ml

1,000

1,200Fig. 2. Capture efficiency using MCF-7 cells withdifferent input cell amounts. Strong linear correlation
between capture efficiency and low sample concentrationsof 10–1,000 cells/ml.

Brightfield

DAPI

PanCK

CD45Single CTC

Merged800

Prostate

Lung

Melanoma

KidneyLeukemia

CNS

Colon

CTC

BreastWBC

RBC

Platelets

Celselect
Slides01,000

10 µm

10 µm

10 µm

10 µm

10 µm

10 µm

10 µm

10 µm

10 µm

CTC Cluster

10 µm

Fig. 3. Fluorescent microscope images of single CTCs and CTC clusters captured and stained inCelselect Slides. Cells positive for 4',6-diamidino-2-phenylindole (DAPI) and pancytokeratin (PanCK), and negative for CD45 arecounted as circulating tumor cells (CTCs) by Rare Cell Analysis Software.

Cell Capture and Recovery

4Improved Utility across Sample Types• 	Liquid biopsies and other cell suspensions are complex and heterogenous. When collecting thecellular fraction from these samples, it is imperative to capture and recover cells in a state suitablefor downstream applications• 	Gentle size-based cell capture with Celselect Slides eliminates the guesswork and enables analysis
of various samples, including many types of cancer cells, immune cells, and other cell types foundin liquid biopsies, with high capture and release efficiencies (Figures 4 and 5)

Capture Efficiency, %Enrichment

Tube Type

CancerCell Line

Enrichment Enumeration

CaptureEfficiency, %Release
Efficiency, %K2-EDTA

8470

BreastMCF-78469

K3-EDTA

84

51*

Melanoma

A375

82

76

Streck(Cell-Free DNABCT)80

91

Prostate

PC390

91

Pancreas

PANC-1

8877

Menarini Silicon
Biosystems,
CellSave83

Glioma

U87MG

9082

Menarini Silicon
Biosystems,
CellRescue

A549

78

80

75

79

H1650

84

62

PAXgene (DNA)

72

80

H1975

90

57

Heparin (Li/Na)*——

Kasumi-1*

2485

Citrate*

—

—

K562

52

46

Colon

SW620

68

74

Ovary

SKOV366

65

79

Lung

Leukemia* Compatible with further protocol optimizations using
Protocol Builder Software Tool.
Fig. 4. Celselect Slides are suitable for many bloodtubes. For both enumeration and enrichment applications,cells from multiple blood collection tube types are captured
with high efficiency without workflow modifications.

* Size of Kasumi-1 cells: 6–10 µm.
Fig. 5. Efficient capture and release. Cells for most cancers in therange of 8–30 µm can be captured and released with high efficiencies.

5Accomplish More.
Genesis Protocol Builder Software Tool
Customize, optimize, or develop new cell isolationand staining methods.
•	Design protocols for application
development• Modify and save existing protocols• Easily share protocols via email or USB
Several tunable parameters:• Reagent dispensing• Pump pressures• Incubation settings

Rare Cell Analysis Software
Automated cell counting with customizable parameters
and reports generated with validated results.
• 	Identify cells based on fluorescent labels
and morphology• 	Compatible with images from multiple
fluorescence microscopes• 	Supports predefined or user-defined
antibody panels• 	Analyze images with up to 6 different
fluorescence channels• 	Graphical output of cell gating• 	Use preset Bio-Rad gating parameters orcustomize for specific user applications

Cell Capture and Recovery

6

Amplify Your Rare Cell Research
Empower a wide range of application and analysis possibilities with the Genesis Systemwith Celselect Slides technology:

Rare Cell/Circulating Tumor Cell Applications• Personalized medicine• Cancer staging and monitoring• Biomarker discovery• Immunotherapy•	Treatment monitoring and resistance mechanisms•	Regenerative medicine• Prenatal testing•	Disease monitoring (autoimmune, infectious)

Molecular Analysis

Cellular Analysis• Droplet Digital PCR (ddPCR )• qPCR• RNA-Seq• Single-cell library prep™

™

• Flow cytometry• Western blotting• Antibodies• Cell culture• Microscopy

7

Learn More.
Ordering Information
Catalog #Description

12019822

Genesis Cell Isolation System, includes Genesis instrument, software, and accessories
necessary to run Celselect Slides assays

17009455

Celselect Slides Enrichment Kit 2.0, includes reagents and consumables for cell capture andrecovery for downstream applications of 4 samples

17009802

Celselect Slides Enumeration Indirect Stain Kit 2.0, includes reagents and consumables for cellcapture and staining of 4 samples via labeled secondary antibody

17009841

Celselect Slides Enumeration Direct Stain Kit 2.0, includes reagents and consumables for thecapture and staining of 4 samples via labeled primary antibody

CEL96001

Genesis System, All-Inclusive Service with Preventative Maintenance, includes 1-year
on-site Extended Coverage Service Plan. Unlimited repair including travel, parts, and labor.
Includes annual preventative maintenance

CEL96005

Genesis System, Annual Preventative Maintenance, includes on-site Preventative Maintenance
one-time annual service; travel and labor included

Visit bio-rad.com/Celselect to learn more about
the Genesis System and Celselect Slides.
For research use only.
BIO-RAD, CELSELECT SLIDES, DDPCR, DROPLET DIGITAL, and DROPLET DIGITAL PCR are trademarks of Bio-Rad Laboratories, Inc. in certain jurisdictions.
All trademarks used herein are the property of their respective owner. © 2024 Bio-Rad Laboratories, Inc.

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