Food, Beverage, and Water Safety Testing

CFX Opus
Real-Time PCR Systems

Visit bio-rad.com/FoodScienceCFXOpus for more information.

CFX OPUS REAL-TIME PCR SYSTEMSCONSISTENCYMODERNIZEDThe CFX Opus Real-Time PCR Systems are the next evolution in quantitative PCR (qPCR)
from Bio-Rad Laboratories. With improved thermal performance and our proprietary,
accurate optical shuttle system, your data will be more consistent. A sleek, modern design
includes a refreshed and easy-to-use interface with more flexible connectivity options fordata management and instrument control.

Search your mobile app store for CFX Opus to see, using augmented reality, how
the system fits into your lab, or visit bio-rad.com/CFXOpus for links and details.

2 | BIO-RAD LABORATORIES, INC.

CFX OPUS REAL-TIME PCR SYSTEMSUNIFORM
THERMAL CYCLING
Temperature, °C

Superior Uniformity
We took a great block and made it even better.
The CFX Opus Systems use an improved version of
the CFX Touch Systems’ block to offer exceptional
performance and uniformity while keeping compatibility
with our consumables. With the best thermal uniformity
and accuracy Bio-Rad has ever produced, you’ll neverworry about using the block from edge to edge again,even with highly sensitive assays.

9075100Time, sec

Superior thermal uniformity for reproducible results. The temperatures measured byprobes in 15 wells across a sample block vary by ±0.3°C for the CFX Opus 96 and
CFX Opus Deepwell Systems.

RFU103102

101020Cycles

304062°C
10430103

25Cq

Efficient Optimization
Determining the optimal temperature for primer annealing
is crucial for efficient and specific target amplification.
The thermal gradient feature of the CFX Opus Systemsallows you to optimize your assay in a single experiment,
minimizing the use of precious samples and reagents andsaving valuable research time. At any step in a protocol,users can program a temperature gradient of up to 24°C
across the reaction block, with exceptional temperature
uniformity and reproducibility within each gradient zone.

0

Excellent uniformity. IL-1b plasmid template diluted to 105 copies/reaction amplified
in the presence of a FAM Dye−labeled detection probe with iQ Supermix. Graph shows
96 replicates of 10 μl reactions. Average quantification cycle (Cq) = 19.81 ± 0.10.
RFU, relative fluorescence units.

RFU

Bio-Rad’s patented reduced-mass sample block heats and cools
more quickly than standard blocks, improving thermal uniformity
and minimizing edge effects.

102— SYBR® Green: E = 98.7%

20
151001020Cycles

30

140

2345log starting quantity

6104

30

103

25Cq

RFU56°C102— SYBR® Green: E = 93.6%

20
151001020Cycles

30

40123456log starting quantity

Thermal gradient experiment for optimizing annealing temperature. A tenfold dilution
series (10 6 to 10 copies) of plasmid containing GAPDH template was amplified in thepresence of SYBR® Green using a protocol with an annealing thermal gradient ranging
from 55 to 68°C. Results are presented for two temperatures, showing 62°C as optimal inthis case. Cq, quantification cycle; RFU, relative fluorescence units.

3 | BIO-RAD LABORATORIES, INC.

CFX OPUS REAL-TIME PCR SYSTEMSROBUSTOPTICAL DESIGN

Multiple Data Acquisition Modes
The CFX Opus Systems can acquire data using several modes.
Choose to acquire data for SYBR® Green I, EvaGreen®, andsingle-color FAM protocols using the fast scan mode or choose
to acquire data from all channels when performing multiplex
protocols. The CFX Opus Systems include one channel with anLED-filter photodiode combination designated for single-color
fluorescence resonance energy transfer (FRET) experiments,
further expanding your experimental options. FRET mode canexpand your experimental options to applications such as protein
thermal shift (melt) analysis.

Excitation
Reporter dye:

Channel 1
450–490
FAM

Channel 2515–535HEX

Channel 3
560–590
Texas Red

Channel 4 Channel 5*
620–650
672–684
Cy5Quasar 705

1.00
0.90
0.80
Normalized absorbanceFive-Target Multiplexing
The CFX Opus Systems can discriminate among up to five targets
in a single reaction well. The optical filter sets are designed tomaximize fluorescence detection for specific dyes in specificchannels. At every position and with every scan, the optics shuttle
is reproducibly centered above each well so the light path is always
fixed and optimal. There is no need to sacrifice data collection in
one of the channels to normalize to a passive reference.

Discrete Channels for Multiplex Data Acquisition

0.70
0.60
0.50
0.40
0.30
0.20
0.100.00
425

450500Detection

550600

Wavelength, nm

Channel 1 Channel 2510–530 560–580
FAM
HEX

Reporter dye:

650

Channel 3
610–650
Texas Red

700

750

775

Channel 4 Channel 5*
675–690 705–730
Cy5Quasar 705

1.00
0.90
0.80
Normalized absorbanceThe solid-state optical technology of the CFX Opus Systems
provides sensitive detection for precise quantification and target
discrimination. Scanning just above the sample plate, the optics
shuttle individually illuminates and detects fluorescence from eachwell with high sensitivity and consistency. In either acquisitionmode, the optical system automatically collects data from all wells,
so you can enter or edit well information on your own schedule
without fear of losing data to annotation mistakes.

0.70
0.60
0.50
0.40
0.30
0.20
0.10
0.00
425

450

500

550600

Wavelength, nm

650700750775* Channel available only on the CFX Opus 96 and CFX Opus Deepwell Systems.

Exceptional Performance of Five-Target Multiplex
40

Quantification cycle (Cq)353025201510–3As the CFX Opus Systems’ optics shuttle travels across the plate, light isfocused into the center of each sample well. Side view of the optics shuttle shows
the 450–490 nm LED firing and SYBR® Green emitting at 520 nm into the detector.

Reporter Dye— FAM— HEX— Texas Red— Cy5— Cy5.5

Assays
ACTB
NGFRP
TBP
EF1a
GAPDH

–2

–1
01log starting quantity, ng

Efficiency, %
99.0
100.5
97.6
96.6
96.9

2

R2
1.000
0.999
0.997
0.999
1.000

3Slope–3.346–3.311–3.382–3.406–3.397The linear dynamic range in 5-plex one-step reverse transcription qPCR
(RT-qPCR) reactions. Five targets across 7 orders of magnitude (1 pg to 1 μg
input RNA) were amplified using Reliance One-Step Multiplex Supermix on a
CFX Opus 96 Real-Time PCR System. The results demonstrated exceptional
performance with superior efficiency and linearity over a wide dynamic range.

BIO-RAD LABORATORIES, INC. | 4

CFX OPUS REAL-TIME PCR SYSTEMSPOWERFULSOFTWARE

CFX Manager Software, Industrial Diagnostic Edition (IDE), controls all operations for the CFX Opus 96 and CFX Opus Deepwell
Real-Time PCR Systems. Globally validated by international bodies, this powerful software delivers the flexibility and ease of use to run
all of our food, beverage, and water assays while providing you with the confidence you require for your testing needs.

Intuitive User Interface■ 
Predefined applications fortarget analytes

Secure and Customizable Reports■ Reports with all sample andcontrol data■

Multiple assays on the same plate■

Automatic email of reports■

Batch and lot traceability■

Color customization of results■

 ull integration with the iQ-Check
FPrep Solution

Automated Analysis and Interpretation■ Simple start-and-go analysis■■■

 utomatic qualitative andA
quantitative interpretation■

Open Platform with CFX MaestroSoftware■ Advanced analytical capabilities■Accurate and reliable results
 idirectional communication with aBlaboratory information management
system (LIMS) ddition of company logos
Ato reports

 bility to run customized/homebrew
A
PCR assays■

Compatibility with iQ-Design Assays■

Freedom to operate

BIO-RAD LABORATORIES, INC. | 5

CFX OPUS REAL-TIME PCR SYSTEMSGET THE SPECIALIZED BLOCKFOR YOUR WORKFLOW
96 Well
This is our most common
block for general throughput
and maximized multiplexing.
This six-LED system allows
five channels for multiplexing
and an additional channel
for FRET applications.

DeepwellOur Deepwell format is a 96-well blockwith one of the largest supported reaction
volumes on the market. Its compatibility with
consumables is similar to our other 96-wellsystem, but its deeper wells improve nucleic
acid detection in workflows that require larger
reaction volumes. The Deepwell format canalso multiplex up to five targets simultaneously
and supports FRET applications.

Talk to your representative about which format is best for you and see our specifications sheet for more details on reaction volumes
and plastic consumables compatibility.

Ordering Information
Catalog #Description

17007992	
CFX Opus 96 Real-Time PCR System, includes CFX Opus 96
System and communication cables
17007991	
CFX Opus Deepwell Real-Time PCR System, includes
CFX Opus Deepwell System and communication cables
3593893	
CFX Manager Software, Industrial Diagnostic Edition (IDE),
1 unit12013758
CFX Maestro Software 2.3 for Windows PC, 1 license,
for real-time PCR plate setup, data collection, statistics, andgraphing of results; for Windows PCs
12012832 	CFX Maestro Software 2.3 for Windows PC, Security Edition,
1 license, for real-time PCR plate setup, data collection, statistics,
and graphing of results in a regulated environment; for Windows PCs
12013028 	CFX Maestro Software 2.3 for Windows PC, Security Edition,
5 licenses, for real-time PCR plate setup, data collection, statistics,
and graphing of results in a regulated environment; for Windows PCs

Visit bio-rad.com/FoodScienceCFXOpus for more information
about the CFX Opus Real-Time PCR Systems, PCR consumables,and reagents.
BIO-RAD is a trademark of Bio-Rad Laboratories, Inc. iQ-Check is a trademark of Bio-Rad
Europe GmbH in certain jurisdictions. SYBR is a trademark of Thermo Fisher Scientific Inc.
EvaGreen is a trademark of Biotium, Inc. All trademarks used herein are the property of theirrespective owner.

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