Real-Time PCRConsistent and Accessible Quantitative
PCR Analysis for Any Laboratory
Powerful Genomic Analysis Platform
for Singleplex and Duplex Assays
The Bio-Rad™ CFX Duet Real-Time PCR System makes
uniform and precise quantitative PCR (qPCR) accessible forany lab. The CFX Duet provides the thermal performanceand the proprietary, accurate optical shuttle system of ourpremium CFX Opus Real-Time PCR Systems for customers
who run only singleplex and duplex assays.
The CFX Duet System utilizes CFX Maestro Software throughan attached computer as its control and analysis system.
CFX Maestro Software makes experiment setup andanalysis easy and provides customized graphing and reports
without exporting to other programs. The economical user
can quickly get started with gene expression, genotyping,thermal gradient optimization, quantification, and even proteincharacterization using fluorescence resonance energy transfer(FRET). The two-color CFX Duet System is factory calibrated
for common dyes such as SYBR® Green and EvaGreen®.
This system is not compatible with the BR.io cloud platform.
Benefits■ No calibration or passive reference dyes required■

■

■

■

Intuitive and robust software
 ersatile capabilities for essential molecular biology andV
protein characterization
 ime-saving experiment optimization with thermal
Tgradient feature ptimal thermal performance equivalent to the premiumO
CFX Opus Systems

Ready Out of the Box

Our consistent distance scanning shuttle technology comesfactory calibrated for common fluorophores and does not
require passive reference dyes, so even labs new to qPCR
will be ready to go upon receiving the system.

Visit bio-rad.com/CFXDuet for more information.

Ease of Use

The CFX Duet System uses the same software as our premium
CFX Opus Systems. CFX Maestro Software makes setup andanalysis easy. Postanalysis data management can be done
within the software, which makes customized graphing andbasic statistical analysis available without having to export files.
Essential Molecular Analysis Is Accessible

Since this two-color system does not require passive referencedyes, it can perform genotyping and many gene expression
analyses. Quantification of up to two targets is possible and an
additional FRET mode enables protein melt analysis for basic
protein characterization experiments.
Efficient Optimization and Thermal Management

The CFX Duet System has thermal gradient functions
across the eight rows of the thermal block. Even with thistwo-channel system, you can optimize thermal parametersin fewer runs. The CFX Duet System has the same thermal
uniformity and precision as our premium CFX Opus Systems,
so every lab can have access to its robust thermal capabilities.

Consistent and Accessible Quantitative PCR Analysis for Any Laboratory

Ordering Information
Catalog #Description

Instrument
12016265

CFX Duet Real-Time PCR System, includes power cord, USB cable

SoftwareAll software for Windows unless otherwise noted.
12013758
CFX Maestro Software, includes USB installation drive, video
quick guides, PDF instruction manual
12012832
12013028
12012833
12012834
1845025
Consumables
HSP9655
HSP9601
MSB1001
TLS0851
TLS0801
TCS0803
Reagents
1845098
12010176
172527017250161725095
17005726
12011928

CFX Maestro Software, Security Edition, 1 license
CFX Maestro Software, Security Edition, 5 licenses
CFX Maestro Software, Russian Edition, 1 license
CFX Maestro Software, Chinese Edition, 1 license
Precision Melt Analysis Software, includes 2 user licenses,
installation CD, 2 HASP HL keys, melt calibration kit
Hard-Shell™ 96-Well PCR Plates, pkg of 50, low profile, thin wall,skirted, white shell/white wells
Hard-Shell 96-Well PCR Plates, pkg of 50, low profile, thin wall,
skirted, white shell/clear wells
Microseal™ 'B' PCR Plate Sealing Film, pkg of 100, optically clear
seals for PCR plates
0.2 ml 8-Tube PCR Strips without Caps, low profile, white
0.2 ml 8-Tube PCR Strips without Caps, low profile, clear
0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear
CFX Qualification Plate, 96-wellReliance One-Step Multiplex RT-qPCR Supermix, 1 ml (1 x 1 ml),
200 x 20 μl reactions
SsoAdvanced Universal SYBR® Green Supermix, 2 ml (2 x 1 ml),
200 x 20 μl reactions
SsoAdvanced Universal Inhibitor-Tolerant SYBR® GreenSupermix, 2 ml (2 x 1 ml), 200 x 20 μl reactions
SingleShot SYBR® Green One-Step Kit for Cell Lysis andRT-qPCR, 100 x 50 μl reactions
SEQuoia Complete Stranded RNA Library Prep Kit, 24 reactions
SEQuoia Dual Indexed Primers Set, 12 vials of unique dual
indexed primers, 96 reactions

Search CFX qPCR Systems in the Apple App Store and Android
Play Store for an augmented reality tour. Contact your local sales
representative for an in-person demonstration.
Visit bio-rad.com/CFXDuet for more information.
BIO-RAD, HARD-SHELL, and MICROSEAL are trademarks of Bio-Rad Laboratories, Inc. incertain jurisdictions. All trademarks used herein are the property of their respective owner.
© 2023 Bio-Rad Laboratories, Inc.
EvaGreen is a trademark of Biotium, Inc.
SYBR is a trademark of Thermo Fisher Scientific Inc. Bio-Rad Laboratories, Inc. is licensed
by Thermo Fisher Scientific Inc. to sell reagents containing SYBR Green I for use in realtime PCR, for research purposes only.

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