BIO-RAD

Virus Detection and Transmission Kit

Solve the mystery of how a virus spreadthrough a restaurant

CONCEPTS• Chain of Infection• Virology• Molecular Diagnostics• PCR• Reverse-Transcription
PCR• Gel Electrophoresis

SKILLS• Agarose Gel
Electrophoresis• Data Collectionand Analysis

Two patients stumble into a hospital with symptoms associated with a newly emerging virus.
Doctors diagnose the patients and find one is infected with the novel virus. This person had been ata local restaurant earlier that day, so public health officials track down others who were also at therestaurant to determine how the virus spreads. Based on published viral transmission case studies,
this hands-on lab activity puts your students into the roles of an emergency room (ER) doctor,
medical laboratory scientist, epidemiologist, and public health official as they analyze patient samples
and use patient information to determine how a novel virus strain spread through a restaurant.

Your Choiceof Virus andTransmission
Scenario
Explore the chain ofinfection and mode oftransmission of yourchoice of either a novel
norovirus or a novel
coronavirus. Once you’vechosen the virus, choose
one of two possible
transmission scenarios
for a total of four different
activity options.

Career Highlights

No PCR Required

Use real-world scenarios
to explore viral biology,
pathophysiology,
molecular diagnostic
techniques, andepidemiology. Career
highlights introduce
students to a variety ofopportunities within thehealth and life sciences.

Use the included preamplified DNA samples
to teach diagnostic
PCR applications
without a thermal cycler.
Analyze simulated
samples by agarose
gel electrophoresis,
compile class data, andthen analyze infection
patterns and other patient
information to deduce themode of virus transmission.

BIO-RADWas it something they ate?
Activities include• Pre-lab activities:— Diagnose hypothetical patients based on their symptoms— Explore virus structure and pathogenesis— Understand the basis for reverse-transcription diagnostic tests• Laboratory investigation — perform gel electrophoresis and analysis of pre-amplified
restaurant patron and staff samples• Post-lab activity — analyze class and restaurant patron/staff details to determine
the mode of transmission• Optional activity (coronavirus) — consider what might need to be done to prevent
further spread of the virus

Evaluate patient
symptoms

Analyze patientswab samples

Kit content supports 8 workstations• Pre-amplified DNA samples• Molecular weight ruler• EZ Micro Test Tubes• Orange G loading dye, 5x• Some configurations include electrophoresis reagents and either Fast Blast DNA
Stain or UView 6x Loading Dye and Stain

Required accessories not included in kit• Adjustable-volume micropipets and tips (20–200 µl)• Adjustable-volume (2–20 μl) or 10 μl fixed-volume micropipet/s and tips
• Horizontal gel electrophoresis chambers and gel staining trays• Power supply• UV transilluminator (if using UView 6x Loading Dye and Stain)• Microcentrifuge racks, marking pens, and calculators

Unravel thetransmission mystery

Timeline• Finish all activities in two to four 45-minute class periods• Complete the electrophoresis step in 10 minutes with our Fast DNA Gel Protocol 		(see bulletin 5396)
Ordering Information
Catalog #Description

17008261EDU		

Virus Disease Detection and 		Transmission Kit

17008251EDU				

Virus Disease Detection and 		Transmission Kit plus Fast Blast 		Electrophoresis Reagents​

17008241EDU				

Virus Disease Detection and 		Transmission Kit plus UView 		
Electrophoresis Reagents

Evaluate patient
symptoms

Analyze patientswab samples

Unravel thetransmission mystery

Call 1-800-4BIORAD (1-800-424-6723) or visit bio-rad.com/VDTKit for more information and classroom resources.
BIO-RAD is a trademark of Bio-Rad Laboratories, Inc. All trademarks used herein are the property of their respective owner. © 2023 Bio-Rad Laboratories, Inc.

Bulletin 3537 Ver A 23-0518 0723