Ion Exchange Chromatography

Got Purification Troubles?

Improve H1N1 Virus Purification Efficiency with the Right Resin
The Challenge
Does your H1N1 virus culture supernatant require nuclease digestion prior to column loading? Does
this pretreatment affect your virus recovery? Do you want to reduce your process purification costs?

Purification Tip
A chromatographic approach using a high-capacity anion exchange resin that removes nucleic acids
will eliminate the requirement for a costly nuclease treatment step.

Our Approach
Microfiltered influenza A virus subtype H1N1 culture supernatants with and without nuclease treatment
were loaded onto a 1 ml Foresight Nuvia HP-Q Column as described previously (see bulletin 7375) andtheir purification performances were compared. Figure 1A shows that with Nuvia HP-Q Resin there is no benefit
from nuclease treatment; total hemagglutinin (HA) recovery and removal of host cell proteins (HCP) and host cellDNA (hcDNA) impurities were the same with and without Benzonase. Furthermore, Figure 1B demonstrates thathigher viral RNA recovery can be achieved with Nuvia HP-Q Resin without Benzonase. These results demonstratethat a nuclease step can be avoided by using Nuvia HP-Q Resin chromatography for H1N1 virus purification.

1009080706050403020100B. H
 1N1 viral RNA recovery following elution fromNuvia HP-Q Resin

Viral RNA recovery, %Performance, %A. H1N1 recovery and purity with/without Benzonase
treatment before Nuvia HP-Q chromatography

HA recovery

HCP removal

hcDNA removal

1009080706050403020100With Benzonase

Without Benzonase

Fig 1. H1N1 virus purification with Nuvia HP-Q Resin. A, performance of Nuvia HP-Q Resin for HA recovery and impurity removal,and B, subsequent RNA recovery from the 590 mM NaCl elution fraction, with ( ■ ) and without ( ■ ) Benzonase treatment of the H1N1virus cell culture supernatant. HA, hemagglutinin; HCP, host cell proteins; hcDNA, host cell DNA.

Go to bio-rad.com/NuviaHPQ and explore Nuvia HP-Q Resin.

Got Purification Troubles? Improve H1N1 Virus Purification Efficiency with the Right Resin

Innovative Products. Customized Solutions.
Nuvia HP-Q Resin

Ordering Information

Nuvia HP-Q Resin will put you on your path to:Catalog #12006693
12006691
12006660
12006659
12007023
12007022
12007018
12007019
12007033
12006994
12009282
12007020
12007021
12007013
12007014
12006908

More productive large biomolecule purification
High-throughput purification processes■ Better reproducibility between processes■■

Go to bio-rad.com/NuviaHPQ and explore
the features of Nuvia HP-Q Resin.

Description
Nuvia HP-Q Media, 25 ml
Nuvia HP-Q Media, 100 ml
Nuvia HP-Q Media, 500 ml
Nuvia HP-Q Media, 5 L
Nuvia HP-Q Media, 10 L
Nuvia HP-Q Media, benzyl alcohol (B.A.), 25 ml
Nuvia HP-Q Media, B.A., 100 ml
Nuvia HP-Q Media, B.A., 500 ml
Nuvia HP-Q Media, B.A., 5 L
Nuvia HP-Q Media, B.A., 10 L
EconoFit Nuvia HP-Q Column, 1 x 1 ml
Foresight Nuvia HP-Q Column, 1 ml
Foresight Nuvia HP-Q Column, 5 ml
Foresight Nuvia HP-Q RoboColumn Unit, 200 µl
Foresight Nuvia HP-Q RoboColumn Unit, 600 µl
Foresight Nuvia HP-Q Plates, 2 x 96-well, 20 μl

Services and Support
We offer multiple service and support levels
to deliver solutions tailored to your needs.
Method developmentProcess development■ Column packing support■ Global technical support■■Process purification bottles, columns, and plates.

Global Supplier

Screen Our Process Resins Today

We are a global supplier of process resins and columns withmore than 30 support sites across six continents. Contact
your regional Bio-Rad Process Chromatography Specialistby email at process@bio-rad.com or call our customer
service at 1-800-4-BIORAD (1-800-424-6723). Connect
with us for news, events, and the latest product information.

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and request a sample today.
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