Chromatography Resins

DNA Sample Preparation Using Chelex 100 Resinfor Forensic and Population Genetic Analyses
Publications List

Chelex 100 Resin has long been used for fast and easy DNA preparation from many sample types
ranging from cell cultures to tissues.
Chelex 100 Resin, and the related InstaGene Matrix, offer cost-effective alternatives to commercial DNA
or RNA extraction kits. Chelex 100–based protocols require far fewer consumables than do extraction
kits, thus generating significantly less waste.
Here we compiled examples of studies that utilized Chelex 100 Resin for forensic or population genetic
analyses over the last 20 years.
Yan W et al. (2022).
Forensic examination and application of areca nuts as material evidence.
J Forensic Sci 67, 321–327.
Perera N et al. (2021).
Development of a 16 X-STR multiplex PCR system for kinship analysis and its applicability for the Sinhalese population in Sri Lanka.
Int J Legal Med 135, 161–166.
Alqahtani A et al. (2020).
Isolation and next generation sequencing of archival formalin-fixed DNA.
J Anat 237, 587–600.
Ferreira IR et al. (2020).
A newborn screening pilot study using methylation-sensitive high resolution melting on dried blood spots to detect Prader-Willi
and Angelman syndromes.
Sci Rep 10, 13,026.
Pankham A and Tatu T (2020).
Detecting HbE gene using DNA extracted from urine sediments by Chelex-plus-heating technique.
J Biomol Tech 31, 81–87.
Wang WH et al. (2020).
Establishment and evaluation of a novel method based on loop-mediated isothermal amplification for the rapid diagnosisof thalassemia genes.
Risk Manag Healthc Policy 13, 303–311.

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DNA Sample Preparation Using Chelex 100 Resin for Forensic and Population Genetic Analyses Publications List

Brito FCA et al. (2019).
DNA extraction of urinary bladder swabs collected from carbonized and decomposing corpses: Possible applicationin disaster victim identification.
Leg Med (Tokyo) 37, 15–17.
Fachet C et al. (2017).
High resolution melt curve analysis based on methylation status for human semen identification.
Forensic Sci Med Pathol 13, 86–91.
Inácio A et al. (2017).
Study of InDel genetic markers with forensic and ancestry informative interest in PALOP’s immigrant populations in Lisboa.
Int J Legal Med 131, 657–660.
Pîrlea S et al. (2017).
Permanganate-assisted removal of PCR inhibitors during the DNA Chelex extraction from stained denim samples.
Int J Legal Med 131, 323–331.
Forsberg C et al. (2016).
High-throughput DNA extraction of forensic adhesive tapes.
Forensic Sci Int Genet 24, 158–163.
Liu Z et al. (2016).
New multiplex real-time PCR approach to detect gene mutations for spinal muscular atrophy.
BMC Neurol 16, 141.
Allam G et al. (2015).
Neonatal infections in Saudi Arabia: Association with cytokine gene polymorphisms.
Cent Eur J Immunol 40, 68–77.
Barbarić L et al. (2015).
Powdered activated carbon: An alternative approach to genomic DNA purification.
J Forensic Sci 60, 1,012–1,015.
Coulson-Thomas YM et al. (2015).
DNA and bone structure preservation in medieval human skeletons.
Forensic Sci Int 251, 186–194.
Oleiwi AA et al. (2015).
The relative DNA-shedding propensity of the palm and finger surfaces.
Sci Justice 55, 329–334.
Yang H et al. (2015).
Incidence and molecular characterization of glucose-6-phosphate dehydrogenase deficiency among neonates for newborn screening
in Chaozhou, China.
Int J Lab Hematol 37, 410–419.
Adayev T et al. (2014).
Fragile X protein in newborn dried blood spots.
BMC Med Genet 15, 119.

© 2022 Bio-Rad Laboratories, Inc.

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DNA Sample Preparation Using Chelex 100 Resin for Forensic and Population Genetic Analyses Publications List

Yue L et al. (2014).
Rapid screening for sickle cell disease by polymerase chain reaction-high resolution melting analysis.
Mol Med Rep 9, 2,479–2,484.
Walsh PS et al. (2013).
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.
Biotechniques 54, 134–139.
Wang PH et al. (2013).
DNA extraction and sex determination for human teeth dated 3000 years ago unearthed in Xi’an.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi 30, 619–621.
Zhu YS et al. (2011).
A study on the relationship between TCTA tetranucleotide polymorphism of the HPRT gene and primary hyperuricemia.
Genet Mol Res 10, 3,121–3,126.
Grsković B et al. (2010).
Population genetic analysis of haplotypes based on 17 short tandem repeat loci on Y chromosome in population sample
from eastern Croatia.
Croat Med J 51, 202–208.
Torres-Serrant M et al. (2010).
Newborn screening for Hermansky-Pudlak syndrome type 3 in Puerto Rico.
J Pediatr Hematol Oncol 32, 448–453.
Butler JM (2009).
DNA extraction from forensic samples using Chelex.
Cold Spring Harb Protoc, 6.
Feng Y et al. (2008).
A method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles.
Fa Yi Xue Za Zhi 24, 435–436.
Ba HJ et al. (2007).
Comparative research of the influence factors of DNA extraction of bloodstain on the filter paper with Chelex-100 method.
Fa Yi Xue Za Zhi 23, 347–348.
Roberts KA and Calloway C (2007).
Mitochondrial DNA amplification success rate as a function of hair morphology.
J Forensic Sci 52, 40–47.
Eminovic I et al. (2005).
A simple method of DNA extraction in solving difficult criminal cases.
Med Arh 59, 57–58.
Rekand T et al. (2003).
Detection of viral sequences in archival spinal cords from fatal cases of poliomyelitis in 1951-1952.
J Virol Methods 114, 91–96.
Tsuchimochi T et al. (2002).
Chelating resin-based extraction of DNA from dental pulp and sex determination from incinerated teeth with Y-chromosomalalphoid repeat and short tandem repeats.
Am J Forensic Med Pathol 23, 268–271.
© 2022 Bio-Rad Laboratories, Inc.

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