SURFE²R 96SE
Automated Transporter Screening

SURFE²R 96SE

SSM-based screening of transporters,
pumps and ligand-gated ion channels
The SURFE²R 96SE (surface electrogenic event

Any membrane can be accessed for recordings,reader) is the only commercially available platform

ranging from isolated membrane vesicles fromfor higher throughput solid supported membrane

native tissues or cultured cells, organellar(SSM) -based electrophysiology on the market.

membranes to transporters reconstituted inproteoliposomes. The SURFE²R 96SE allows for a

Functional and direct recordings of electrogenicdata throughput matching secondary screening

membrane transport is measured in real-time withand characterization of compounds targetinghigh sensitivity and high temporal resolution.

transporters, symporters, exchangers, uniporters
and pumps and from ligand-gated and leak ionchannels.

SURFE²R 96SE

Key features at a glance
1Real-time transporter recordings

5

Up to 10,000 data points per day

2

Parallel recordings from 96 wells

6High success rates >95 %3

No need for labels, radioactivity or
fluorescence7

Low sample consumption

4

High sensitivity & temporal resolution

8>100 validated targets from diverse
membrane sources

SURFE²R 96SE

Matching your transporter
screening needs
Transporter screening and characterization

Hit validation

The SURFE²R 96SE facilitates screening andWith a throughput of up to 10,000 data points

characterization of compounds acting on

per day, the SURFE²R 96SE is a versatile tool for hit

transporters, pumps, ligand-gated and leakvalidation with the unique feature of accessing

channels. High success rates and in-well quality

proteins residing in their native membrane

control options confer efficient dose response

environment, including organelles.

analysis or single point screening of compounds
modulating transporter activity.

Powerful software for recordings and analysis based on the SyncroPatch software.

SURFE²R 96SE

Efficient data handlingand export
SURFControl is a powerful graphical user

DataControl accelerates data analysis byinterface for quick and easy setup of recording

quick and easy loading of recorded files andparameters and experimental protocols.

user-defined analysis templates.

• Compound and experimental details storedwith datapoints• Analysis performed with a few mouse clicks(IC50−, EC50− values etc.)• Real time plot of results• Data analysis templates display results within
seconds• In-well QC options• Color-coding of recording wells based on
QC parameters• Raw data and analysis results in the samewindlow• Instant re-calculation upon parameter changes• Heat maps for intuitive overview of compoundeffects• Customized data export• Word or pdf-reporting

DataControl 96
Scroll through the data, select a data
analysis template or fit parameters.
Results, compound information and QC
parameters are exported in a userdefined export format, ready for further
database integrations.

SURFE²R 96SE

Broad range of validated targets
Primary active
transporters / Ion pumps

Uniporters+ Symporters + Exchangers

ATPases

Inorganic ionsAmino acids + peptides

NaK-ATPase

NhaA/B (2Na+/1H+)

PepT1/PepT_St (di-/tri-peptides)

HK-ATPase

NhaP/NHA2 (1Na+/1H+)

YdgR/DtpA (tri-peptides)

SERCA (Ca2+)

NCX1/mjNCX (3Na+/Ca2+)

YhiP/DtpB (di-/tri-peptides)

v-ATPase (H+)

ecClc/Clc-3/5/7/K (Cl −/H+)

PutP (Na+/proline)

FoF1-ATPase (H )NirC (NO /H )Kdp-ATPase (K )afAmt1-3/ksAmt5/ecAmtB (NH )GltTK (Na+/aspartate)

CopA (Cu )NeRh50 (NH )EAAC1 (3Na+/glutamate)

ATP7A/B (Cu )SulP/DauA (bicarbonate)

PAT1 (H+/proline)

ATP8A2 (Flippase)

NIS (2Na /I )ArcD (arginine/ornithine exchange)

VrPPaseNaPi-IIb (Na+/PO32−)

CAT-IIB (cationic amino acids)

MntH2 (Mn(II), Zn(II), Co(II), Cd(II))

GlyT1/2/B1 (Na+/glycine)

YiiP (metals)

B0AT2 (Na+/neutral amino acids)++2+−2GltP (Na+/glutamate)++4+42+Redox-driven ion pumps+−Cystin Transporter

Complex I
Respiratory chain complex I/III
Respiratory chain complex II/IIISugars

Other organic ions

Cyctochrome c-oxidase

SGLT1/2 (Na+/glucose)

OCT1/2 (organic cations)

Respiratory chain complexes I/III/V

MelB (Na+/melibiose)OATP1B1 (organic anions)

LacY (H+/lactose)

CNT1 (nucleosides)

FucP (H+/fucose)

ANT (ADP/ATP exchange)

XylE (H+/xylose)

GAT1 (Na+/GABA)

GlcP (glucose)

BetP (betaine)

STP10 (H+/glucose)

CHT (choline)

Light-driven ion pumps
Bacteriorhodopsin (BR)Rhodopsin OR1
Rhodopsin-2 (KR2)Halorhodopsin (HR)
Acerhodopsin
Channelrhodopsin 1/2 (ChR)

LicB (choline)
NupC (H+/nucleoside)
PurT (H+/purine)
NaCT (Na+/citrate)
SugE/Gdx (guanidinium)
EmrE (small drugs)

Channels + Pores

MCT13 (monocarboxylates)
PfCRT (cloroquine/CQ)
DgoT (H+/galactonate)

Gramicidine
P2X2
nAChR
A/M2
UCP1 (Slc25a7)
TRPC5
TRPA1
AQP6
KtrAB
TMEM175

Malate/lactate exchange
Bicarbonate transportSURFE²R 96SE

The SURFE²R 96SE includes• Small footprint pipetting platform CyBio® Felix• 96-channel low-noise amplifier• 8 positions for different solutions (1 well to 96 well)• Windows with SURFControl and DataControl software suite• CyBio Composer plus pre-installed workflows• SURFE²R 96SE sensor plates (96-well)• 1 year warranty with further optional comprehensive service plans available• Unmatched application and assay support

Specifications
Sensor stability

Hours – days

Average success rate

>95 %Throughput

Up to 10,000 data points per day

Temporal resolution

ca. 5 ms

Liquid handling robot

CyBio® Felix

Amplifier channels

96Number of pipettes

96

Protein consumption

0.1–1 µg protein per well (total protein)

Compound solution per well

50 – 200 µl

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