No-wash cytokine
assay solutions.
Cytokines play vital roles in both innate and adaptive immunity in which they regulate immune andinflammatory responses and maintain immunological homeostasis. In addition to the immune system,
they also exert diverse functions in other cells. Identification of cytokines, their receptors andsignaling pathways, and application of these novel observations to the clinic has made possible theALPHA is a versatile, bead-based platform that enables you to assay
the most complex samples in one well and with no wash steps.
Excitation
680 nmdevelopment of novel therapies for various immune diseases and related disorders.

Emission615 nm
AnalyteRevvity has the tools you need to further your cytokine research with kits covering the full range ofcytokines: interleukins, interferons (IFN), lymphokines, chemokines, and TNF. Our assays provide afast alternative to ELISA or other measurement methods for researchers in the field of inflammatory
disease or any other inflammation process. Benefit from our comprehensive portfolio of AlphaLISAand HTRF® cytokine assays that offer:• Fast and easy homogeneous format without wash steps• High quality results with minimal sample consumption• Multiple cytokine measurements from a single sample

Biotinylated
Anti-analyteantibody
Streptavidin-coated
Donor Beads

Anti-analyteantibody
Anti-species conjugated
AlphaLISA Acceptor Beads

HTRF is a fast, sensitive, homogeneous, andready to use assay platform with no wash steps.
Excitation

FRET• Robust and stable assay signal for reproducible results

Emission• Ideal for quantitative detection in cell lines like THP1 and primary cells such as PBMCs
Donor Coupled
Antibody

AnalyteAcceptor Coupled
Antibody

Table 1: Human cytokine assays.

AlphaLISA
CCL1Table 1: Human cytokine assays continued.

HTRF

AlphaLISA

AL3099HV, AL3099C, AL3099F

IL-12

AL3116HV, AL3116C, AL3116F

62HIL12PEG, 62HIL12PEH

AL382HV, AL382C, AL382F

62HIL12P40PEG, 62HIL12P40PEH

IL-13

AL240C, AL240F

62HIL13PEG, 62HIL13PEH

62HCCL4PEG, 62HCCL4PEH

IL-15

AL360HV, AL360C, AL360F

62HCCL5PEG, 62HCCL5PEH

IL-17

CCL2/MCP-1

AL244C, AL244F

62HCCL2PEG, 62HCCL2PEH

IL-12/IL-23

CCL3/MIP-1α

AL257C, AL257F

62HCCL3PEG, 62HCCL3PEH

CCL4/MIP-1β

AL258C, AL258F
AL289C, AL289F

CCL5/RANTESHTRF62HIL17PET,
62HIL17PEG, 62HIL17PEH

CCL18AL3106HV, AL3106C, AL3106F

CCL20

62HCC20PEG, 62HCC20PEH

IL-17A

AL3161HV, AL3161C, AL3161F

AL3188HV, AL3188C, AL3188F

62HCCL22PEG, 62HCCL22PEH

IL-17F

AL391HV, AL391C, AL391F

AL349C, AL349F

62HCXC1PEG, 62HCXC1PEH

IL-18

AL3137C, AL3137F

AL3163HV, AL3163C, AL3163CF

62HCX10PEG, 62HCX10PEH

IL-21

AL3043HV, AL3043C, AL3043F
AL320HV, AL320C, AL320F

62HIL22PEG, 62HIL22PEH
62HIL23PEG, 62HIL23PEH

CCL22
CXCL1/GROαCXCL10CXCL11

AL260C, AL260F

IL-22

CXCL13

AL3098HV, AL3098C, AL3098F

IL-23

AL3006HV, AL3006C, AL3006F

CXCL4/PF4

AL398HV, AL398C, AL398F

IL-25

AL3135C, AL3135F, AL3135HVCXCL9/MIG

AL280C, AL280F

IL-28B

AL365HV, AL365C, AL365F

EPO

AL206C, AL206F

IL-31

AL3003HV, AL3003C, AL3003F

G-CSF

AL213C, AL213F

62HGCSFPEG, 62HGCSFPEH

IL-33

AL3042HV , AL3042C , AL3042F

GM-CSF

AL216C , AL216F

62HGMCSFPEG, 62HGMCSFPEISCF

AL3019HV , AL3019C , AL3019F
AL336HV , AL336C , AL336F

IFNα

AL297C, AL297F

62HIFNAPEG, 62HIFNAPEH

TGFβ1IFNβ

AL3191HV, AL3191C, AL3191F

62HIFNBPEG, 62HIFNBPEG

TNFαAL3157HV, AL3157C, AL3157F

IFNγ

AL3153HV, AL3153C, AL3153F

62HIFNGPET, 62HIFNGPEG, 62HIFNGPEH

TRAIL

AL3089HV , AL3089C , AL3089F

TSLP

AL3040HV , AL3040C , AL3040F

IFNλ2

AL3031HV, AL3031C, AL3031F

IL-1α

AL238C, AL238F

62HIL1APEG, 62HIL1APEH

IL-1β

AL3160HV, AL3160C, AL3160F

62HIL1BPET, 62HIL1BPEG, 62HIL1BPEH, 62HIL1BPEJ

62HIL31PEG, 62HIL31PEH

62HTNFAPET, 62HTNFAPEG, 62HTNFAPEH

Biotin FreeIFNγ

AL3154HV, AL3154C, AL3154F

IL-2

AL3156HV, AL3156C, AL3156F

IL-6

AL3025HV , AL3025C , AL3025F

IL-8

AL3165HV, AL3165C, AL3165F

IL-1RA

AL358HV, AL358C, AL358F

IL-2

AL3155HV, AL3155C, AL3155F

IL-3

AL3134HV, AL3134C, AL3134F

IL-4

AL266C, AL266F

62HIL04PEG, 62HIL04PEH

IL-17A

AL3162HV, AL3162C, AL3162F

IL-5

AL267C, AL267F

62HIL05PEG, 62HIL05PEH

TGFβ1

AL361HV , AL361C , AL361F

IL-6

AL223C, AL223F

62HIL06PET, 62HIL06PEG, 62HIL06PEH

TNFα

AL3158HV, AL3158C, AL3158F

IL-8

AL3164HV, AL3164C, AL3164F

62HIL08PET, 62HIL08PEG, 62HIL08PEH

IL-10

AL3159HV, AL3159C, AL3159F

62HIL10PEG, 62HIL10PEH

IL-11

AL293C, AL293F

62HIL11PEG, 62HIL11PEH

62HIL02PET, 62HIL02PEG, 62HIL02PEH

62HIL18PEG, 62HIL18PEH

AlphaLISA available in 100 data point (HV), 500 data point (C), and 5000 data point (F) sizes.
HTRF available in 100 data point (PET), 500 data point (PEG) and 10,000 data point (PEH) sizes.

Table 2: Mouse cytokine assays.

Table 3: Non-human primate cytokine assays.

AlphaLISA

HTRF

CCL2/MCP1

AL509C , AL509F

62MCCL2PEG, 62MCCL2PEI

IL-17A

AL590HV , AL590C , AL590F

CCL4

AL3190HV, AL3190C, AL3190F

IFNγ cynomolgus

AL561HV , AL561C , AL561F

CCL5/RANTES

AL589C , AL589F

CXCL10/IP-10

AL556HV , AL556C , AL556F

TNFα

AL541HV , AL541C , AL541F

CXCL13

AL581HV , AL581C , AL581FTNFα cynomolgus

AL562HV , AL562C , AL562F

IFNα

AL550HV , AL550C , AL550F

IFNβAL586HV , AL586C , AL586F

IFNγ

AL593HV, AL593C, AL593F

IL-1α

62MIFNGPEG, 62MIFNGPEH

IL-1β

AL592HV, AL592C, AL592F

62MIL1BPEG, 62MIL1BPEH

IL-2

AL585C , AL585F

62MIL02PEG, 62MIL02PEH

IL-3

AL587HV , AL587C , AL587F

IL-4

AL567HV , AL567C , AL567F

IL-5

AL569HV , AL569C , AL569F

IL-6

AL504C , AL504F

IL-10

AL502C , AL502F

IL-13

AL565HV , AL565C , AL565F
AL588C , AL588F

IL-17A homodimer

AL540HV , AL540C , AL540F

IL-18

AL3189HV, AL3189C, AL3189F

IL-25

AL570HV , AL570C , AL570F

IL-33

AL566HV , AL566C , AL566F

MCP-2

AL582HV , AL582C , AL582F

TNFα

AL505C, AL505F

62CTNFAPEG, 62CTNFAPEH

Custom Labeling and Custom Assay Development Services
Don’t see the assay you need listed here? Revvity partners with industry and academia professionals
to develop custom solutions and shorten the drug development process using our powerful, accurate
HTRF®, DELFIA® and AlphaLISA® technologies.
Biochemical assays

62MIL06PEG, 62MIL06PEH

ELISA conversion
Excitation
680 nmCell-based assays
Emission
615 nmAnalyte

62MIL17PEG, 62MIL17PEH

IL-17AHTRF

AlphaLISA available in 100 data point (HV), 500 data point (C), and 5000 data point (F) sizes.
HTRF available in 100 data point (PET), 500 data point (PEG) and 10,000 data point (PEH) sizes.

62MIL1APEG, 62MIL1APEH

IL17AlphaLISA

Biotinylated
Anti-analyteantibody

Streptavidin-coated
Donor Beads

Anti-analyteantibody
Anti-species conjugated
AlphaLISA Acceptor Beads

62MTNFAPEG, 62MTNFAPEH

Biotin Free
IL-4 rhesus

AL564HV, AL564C, AL564F

IL-6 cynomolgus, rhesus

AL555HV, AL555C, AL555F

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