Placental Growth Factor
Overview
Summary

ClinicalPlacental growth factor (PlGF) is
primarily expressed in the placenta,
but it can also be found in low levels
in other tissues such as the heart,lung, thyroid, bone, liver, andskeletal muscle. PlGF is a member ofthe vascular endothelial growth
factor family (VEGF). Its key role isto promote angiogenesis in theplacenta,
contributingtothedevelopment and maturation of thevascular system. In other tissues,
PlGF promotes
angiogenesis inresponse to pathological ischemia orinjury.

In clinical laboratory settings, measuring
the circulating levels of PlGF is crucial indiagnosing preeclampsia, along with othermedical parameters. Preeclampsia isresponsible for 10-15% of maternal
deaths worldwide, according to the World
Health Organization (WHO)1 . PlGF levels
are significantly lower in women withpreeclampsia than in those with normal
pregnancies or gestational hypertension(high blood pressure during pregnancy
without signs of organ damage).

PlGF CLIA RUO prototype
Assay Scheme
Quantitative detection of Placental Growth Factor (PlGF) in human serum/plasma
Assay scheme
Two-step assay (quantitative)

(I)

(II)
Triggers
PlGF

RLUs

PMPs

SampleConjugate

Magnetic particle
coated with Werfen
anti-PlGF mAb

serum/plasma

Werfen anti-PlGF-isoluminol

1 World Health Organization (WHO). (2019). Maternal Mortality. Retrieved from https://www.who.int/news-room/fact-sheets/detail/maternal-mortality.

Dose-response in PlGF RUO prototype
Calibrator
Samplepg/mL*

RLU’s

S0
S1
S2
S3
S4
S5
S6

3
12
41
210
756
2069
8792

568
1,618
4,085
18,10868,517
181,964
698,262*Concentration of the calibrator samples obtained with areference method
Figure 1. Calibration curve for CLIA RUO prototype using Werfen
mAbs. Anti-PlGf mAb (1) was used for coating PMPs and Anti-PlGF
mAb (2) labeled with Iso-luminol was used as a detector.
Recombinant PlGF diluted in serum at different concentrations wasused as a calibrator sample set. Dose response curve shows reactivityof the different calibrator samples plotted against the concentration
obtained with a reference method (measured in pg/mL).

Assay Range

S6/S0

1,299

Table 1. Numerical results dose-response calibration curve.
Signal-to-noise and assay range performance evaluation.

Method Comparison of PlGF RUO prototype vs reference assay

Figure 2 & 3. Method comparison for performance evaluation – Pregnant women with and without risk of preeclampsia. Quantitative
correlation of PlGF concentration obtained in PlGF Werfen CLIA RUO prototype of 40 individual serum samples compared to theconcentration determined with the reference method.

Werfen’s Biomaterial offering
Anti-Placental Growth Factor mAb 1 (ref 3000-7060)

Anti-Placental Growth Factor mAb 2 (ref 3000-7061)

Storage: -70ºC
Source: Hybridoma cell Line
Storage buffer: MES , NaCl pH 6,0
Purification method: Protein A
Protein concentration:1 mg/mL
Preservative: None

Storage: -70ºC
Source: Hybridoma cell Line
Storage buffer: MES , NaCl pH 6,0
Purification method: Protein A
Protein concentration:1 mg/mL
Preservative: None

This product is in R&D Stage and is for “Investigation / Research Use Only”. The content within this brochure is provided for
informational purposes.
Contact oem@werfen.com for further technical information and product availability
Version 1 Dec-2023