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⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ╙ภ㧘㧙㧘ේ⪺⺰ᢥஜᐽ㘩ຠਛߩ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦߩ HPLC ಽᨆᴺ᫪↰㇌ᱜ㧘Ძ೑㓉⟤㧘ਛᎹ␞ሶㅒ⋧♽ߩ㜞ㅦᶧ૕ࠢࡠࡑ࠻ࠣ࡜ࡈࠍ૶ߞߡ㧘ஜᐽ㘩ຠਛߩ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜
ࡒࡦߩಽᨆᴺࠍᬌ⸛ߒߚ㧚ஜᐽ㘩ຠਛߩ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦߪࡔ࠲ࡁ࡯࡞ࠍ
↪޿ߡ᛽಴ߒ㧘Bond Elut Certify ࠞ࡯࠻࡝࠶ࠫ(࿕⋧᛽಴ᴺ)ࠍ↪޿ߡ 2 㧑ࠕࡦࡕ࠾ࠕ᳓-ࡔ࠲ࡁ࡯࡞ṁᶧߢࠢ࡝࡯ࡦࠕ࠶ࡊߒߚ㧚㜞ㅦᶧ૕ࠢࡠࡑ࠻ࠣ࡜ࡈߪࠞ࡜ࡓߦ LiChrosorb RP18 (4.6 x 150mm, 5microns)ࠍ 㧘⒖േ⋧ߦ pH7 ߩ 0.02mol/L ࡝ࡦ㉄Ⴎ✭ⴣᶧ㧛ࡔ࠲ࡁ࡯࡞㧔15:85㧘v/v㧕ࠍ↪޿㧘᷹ቯᵄ㐳 225nm ߢಽᨆߒߚ㧚ᧄᴺߦࠃࠆஜᐽ㘩ຠ߆ࠄߩ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦߩ࿁෼₸ߪߘࠇߙࠇ 88
㧙 96%෸߮ 87 㧙 91%㧘ቯ㊂ਅ㒢୯ߪߘࠇߙࠇ 0.001mg/g ߢ޽ߞߚ㧚㨇ࠠ࡯ࡢ࡯࠼ : ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ㧘ࠪࡉ࠻࡜ࡒࡦ㧘HPLC㧘࿕⋧᛽಴ᴺ㧘ஜᐽ㘩ຠ㧘㘩᰼ᛥ೙೷㨉㧝ߪߓ߼ߦ

C17H26ClN㧔 m.w. 279.85㧕 ߢ޽ࠆ㧚ᐔᚑ 15 ᐕ 11 ᦬㧘⑔ጟ⋵ౝߩ 2 ᬺ⠪߇⚊⼺⩶࠳ࠗࠛ࠶
࠻ߣ⒓ߒߡࠗࡦ࠲࡯ࡀ࠶࠻⽼ᄁߒߚ㧘޿ࠊࠁࠆஜᐽ㘩ຠ߆ࠄ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ޽ࠆ޿ߪࠪࡉ࠻࡜ࡒࡦ
ߩ޿ߕࠇ߆߇ᬌ಴ߐࠇߚ㧚ෘ↢ഭ௛⋭㧘ችፒ⋵㧘ንጊ⋵෸߮⑔ጟ⋵ߩ⺞ᩏߢ㧘⹥ᒰຠߪਛ࿖↥ේ᧚ᢱࠍャ౉ߒ࿖
ౝߢ㍤೷ൻߒߚ߽ߩߢ޽ࠅ㧘৻‛ᄙฬ⒓ߢᄙ⒳㘃ߩ⵾ຠ߇޽ࠆߎߣ߇್᣿ߒߚ㧚⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ

ࠪࡉ࠻࡜ࡒࡦߪ⢈ḩ∝ߩᴦ≮⮎ߣߒߡ↪޿ࠄࠇࠆ㘩᰼ᛥ೙೷ߢ޽ࠆ 1), 2), 3), 4)㧚 1997 ᐕߦ☨࿖ߦ߅޿ߡᛚ⹺ߐࠇߚ
Meridia㧔 ໡ຠฬ㧕ߦߪ㧘ࠪࡉ࠻࡜ࡒࡦߩႮ㉄Ⴎ߇ 1 ࠞࡊ࠮࡞ᒰߚࠅ 5㧘 10 ෸߮ 15mg ฽߹ࠇߡ޿ࠆ㧚Meridia
ߩᛩਈ㊂ߪೋ࿁Ფᦺ 10mg ߆ࠄ㐿ᆎߒ㧘ᢙㅳ㑆ᓟߦ 15mg
߹ߢჇ㊂ߔࠆ㧚ᣣᧄߢߪ㧘ࠛ࡯ࠩࠗ㧔ᩣ㧕ߣ࠼ࠗ࠷ߩࠢ
ࡁ࡯࡞ AG ␠߇౒หߢ⥃ᐥ㐿⊒ࠍⴕ޿㧘2005 ᐕߩ⊒ᄁࠪࡉ࠻࡜ࡒࡦ

߇੍ቯߐࠇߡ޿ࠆ㧚ࠪࡉ࠻࡜ࡒࡦߣߘߩઍ⻢‛ߪࡁ࡞ࠕ࿑ 1࠼࡟࠽࡝ࡦߣ࠮ࡠ࠻࠾ࡦߩౣๆ෼ࠍ㒖ኂߔࠆߎߣߦࠃࠅ

ൻቇ᭴ㅧᑼ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦߩ

㘩᰼⺞▵♽ߩਛᨔߦ૞↪ߒ㧘ḩ⣻ᗵࠍ੣ㅴߐߖࠆ㧚ࠪࡉ࠻࡜ࡒࡦߩ೽૞↪ߣߒߡⴊ࿶਄᣹㧘ᔃᜉᢙჇട㧘㗡∩㧘ࠪࡉ࠻࡜ࡒࡦߦ㑐ߔࠆ⹜㛎ᴺߣߒߡ㧘ⴊਛߩࠪࡉ࠻࡜

ญᷢ㧘ଢ⒁㧘㥦Ἳ╬߇ߺࠄࠇࠆ߇㧘㘩᰼ᛥ೙೷ߩ࠺࠶ࠢ

ࡒࡦઍ⻢‛ࠍㅒ⋧ࠞ࡜ࡓߣ 0.1%࠻࡝ࡈࡠࡠ㈶㉄࡮ࠕ࠮ࠬࡈࠚࡦࡈ࡞࡜ࡒࡦߦߺࠄࠇࠆࠃ߁ߥ⢖㜞ⴊ࿶∝߿ᔃ⤳࠻࠾࠻࡝࡞㧛 0.1%࠻࡝ࡈࡠࡠ㈶㉄(55:45, v/v)ߩ ⒖േ⋧5)6)

ᑯ⤑∝ߩ೽૞↪ߪ⹺߼ࠄࠇߥ޿ 㧚⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ

ࠍ↪޿ߡ LC/MS/MS ߢ ᷹ቯߔࠆᣇᴺ ߣ㧘ஜᐽ㘩ຠਛߩ࠻࡜ࡒࡦߪࠪࡉ࠻࡜ࡒࡦߩਥⷐߥઍ⻢‛ߩ㧝ߟߢ޽ࠆ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ 7)෸߮ࠪࡉ࠻࡜ࡒࡦ 8)ࠍㅒ߇㧘࿖ౝᄖߢක⮎ຠߣߒߡᛚ⹺ߐࠇߡ޿ߥ޿㧚⋧ࠞ࡜ࡓߣ 0.1%ࠡ㉄ࠕ࠮࠻࠾࠻࡝࡞ߩ⒖േ⋧ࠍ↪޿ߡ

࿑ 1 ߦ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦ

LC/MS ߢ ᷹ቯߔࠆᣇᴺ߇ႎ๔ߐࠇߡ޿ࠆ㧚ㄭᐕ㧘࿖᳃ߩൻቇ᭴ㅧᑼࠍ␜ߔ㧚ൻቇᑼߪ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜

ߩஜᐽᔒะߦ઻ߞߡ޿ࠊࠁࠆஜᐽ㘩ຠߦක⮎ຠ߇ᷝടߐࡒ ࡦ ߇ C15H22ClN㧔 m.w. 251.79㧕㧘 ࠪ ࡉ ࠻ ࡜ ࡒ ࡦ ߇ࠇߚ߽ߩ߇㧘ᐫ㗡߿ㅢା⽼ᄁߢᵹㅢߔࠆࠃ߁ߦߥߞߚ㧚⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲ㧔‫ ޥ‬ᄥቿᐭᏒᄢሼะ૒㊁㧕 ߎߩࠃ߁ߥήᛚ⹺ή⸵นක⮎ຠ޽ࠆ޿ߪஜᐽⵍኂ߇⊒↢࠲ࡁ࡯࡞ߪ๺శ⚐⮎Ꮏᬺ㧔ᩣ㧕⵾ߩ HPLC ಽ ᨆ↪ࠍ↪

ߔࠆ߅ߘࠇ߇޽ࠆ⵾ຠߦߟ޿ߡ㧘⪺⠪ࠄߪஜᐽⵍኂࠍᧂ

޿ߚ㧚ὼߦ㒐ߋߚ߼㧘ක⮎ຠ╬ߩᚑಽᬌᩏࠍታᣉߒߡ޿ࠆ㧚ߒ߆ߒ㧘ஜᐽ㘩ຠਛߩ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪ

2 㧑ࠕࡦࡕ࠾ࠕ᳓ -ࡔ࠲ࡁ࡯࡞ṁᶧߪࠕࡦࡕ࠾ࠕࠍ 2㧑฽߻ࠕࡦࡕ࠾ࠕ᳓-ࡔ࠲ࡁ࡯࡞ṁᶧࠍ⺞⵾ߒߚ㧚ࡉ࠻࡜ࡒࡦࠍ HPLC ߢ᷹ቯߔࠆᣇᴺߪႎ๔ߐࠇߡ޿ߥ
޿㧚Ꮢ⽼ߩஜᐽ㘩ຠߦߪᄙ⒳㘃ߩᅹኂᚑಽ߇ሽ࿷ߔࠆߚ

㧞࡮㧞

ⵝ⟎

߼㧘HPLC ࠍ↪޿ߡಽᨆߔࠆߦߪ♖⵾߇ᔅⷐߢ޽ࠆ㧚ߘ⚡ᄖๆశᐲᬌ಴ེ㧔 SPD-10AV㧕 ෸߮࠺࡯࠲ಣℂⵝ⟎ߎߢ㧘࿕⋧᛽಴ᴺߢࠢ࡝࡯ࡦࠕ࠶ࡊߒ㧘HPLC ߢಽᨆߔ

㧔C-R3A㧕ઃ HPLC ߪፉᵤ⵾૞ᚲ㧔ᩣ 㧕⵾ߩ LC10A ࠍ 㧘ࠆᣇᴺࠍᬌ⸛ߒߚ㧚ࠞ࡜ࡓߪ GL ࠨ ࠗࠛࡦࠬ㧔ᩣ㧕⵾ߩ LiChrosorb RP18
㧔4.6×150mm, 5 Ǵ m㧕ࠍ૶↪ߒߚ㧚

㧞

ታ㛎ᣇᴺ

㧞࡮㧝

⹜⮎

㧞࡮㧟

ታ㛎ᠲ૞

ࠪࡉ࠻࡜ࡒࡦᮡḰຠߪ Alexis ⵾ ߩࠪࡉ࠻࡜ࡒࡦႮ㉄㧞࡮㧟࡮㧝Ⴎ㧔৻᳓๺‛㧕ࠍ↪޿ߚ㧚⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ

ࡒࡦߩಽᨆᴺ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜

ߩᮡḰຠߪᏒ⽼ߐࠇߡ޿ߥ޿ߚ߼㧘⚊⼺⩶࠳ࠗࠛ࠶࠻ߩ

ஜᐽ㘩ຠ 0.2g ࠍ 10ml ߩ⹜㛎▤ߦ⒊㊂ߒ㧘ࡔ࠲ࡁ࡯࡞

⹥ᒰຠ㧔໡ຠฬ㧦⟤⊛りḮ㧕߆ࠄ♖⵾ߒߚ㧚⣕㧺㧙ࠫࡔ

2ml ߢ 2 ࿁㧘 10 ಽ㑆ᝄߣ߁᛽಴ߒߚ㧚 ㆙ᔃಽ㔌㧔2500࠴࡞ࠪࡉ࠻࡜ࡒࡦߪ࿑ 1 ߦ␜ߒߚࠪࡉ࠻࡜ࡒࡦߩ⣕㧺㧙

rpm㧕ߒߚᓟࡔ࠲ࡁ࡯࡞ጀࠍណขߒ㧘 0.1 㧑㈶㉄ࠍട߃ࠫࡔ࠴࡞૕ߩൻቇ᭴ㅧߢႮၮᕈ‛⾰ߢ޽ࠆ㧚ߎߩߎߣ߆

ߡ 10ml ߣߒߚ㧚Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫߦࡔ࠲

ࠄ㧘⟤⊛りḮࠍ☳⎈ߒ㧘ߘߩ 8g ߦ 10 㧑ࠕࡦࡕ࠾ࠕ᳓

ࡁ࡯࡞ 2ml㧘᳓ 2ml㧘 0.1 㧑㈶㉄ 2ml ࠍ㗅ᰴᵹߒࠦࡦ࠺

25ml ࠍട߃ߡႮၮᕈߣߒ㧘ࠛ࡯࠹࡞ 10ml ߢ 3 ࿁⿥㖸ᵄ࡚ࠪ࠾ࡦࠣߒߚ㧚ߎࠇߦ᛽಴ṁᶧ 10ml ࠍᵹߒㄟߺ㧘⣕᛽಴ࠍⴕߞߚ㧚64g ߩ⟤⊛りḮ߆ࠄ᛽಴ߒߚࠛ࡯࠹࡞ṁ㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦࠍ଻ᜬߐߖ

ᶧࠍวࠊߖỚ❗ੇ࿕ߒߚᓟ㧘ੇ࿕‛ਛߩ⣕㧺㧙ࠫࡔ࠴࡞ߚᓟ㧘᳓ 2ml ෸߮ࡔ࠲ࡁ࡯࡞ 2ml ߢᵞᵺߒߚ㧚2 㧑ࠕࡦࠪࡉ࠻࡜ࡒࡦࠍ 20ml ߩࡔ࠲ࡁ࡯࡞ߢ 2 ࿁᛽಴ߒߚ㧚᛽ࡕ࠾ࠕ᳓ -ࡔ࠲ࡁ࡯࡞ṁᶧ 4ml ࠍᵹߒ⣕㧺㧙ࠫࡔ࠴࡞ࠪ

಴ᶧࠍỚ❗ੇ࿕ᓟ㧘10ml ߩࠛ࡯࠹࡞ߦṁ߆ߒ㧘2N ᳓㉄ࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦࠍṁ಴ߒߚ㧚ṁ಴ᶧߪ⓸⚛

ൻ࠽࠻࡝࠙ࡓṁᶧ 30ml ߢシߊᵞᵺߒߚ㧚ࠛ࡯࠹࡞ጀࠍ

ࠟࠬ᳇ᵹਅ 40 㧙 50 ͠ߢ 2ml એਅ߹ߢỚ❗ᓟ㧘ࡔ࠲ࡁಽขᓟ㧘᳓ጀߪࠛ࡯࠹࡞ 10ml ߢ 2 ࿁᛽಴ߒߚ㧚᛽಴ᶧ࡯࡞ࠍട߃ߡ HPLC ↪ṁᶧࠍ⺞⵾ߒߚ㧚ࠍỚ❗ੇ࿕ᓟ㧘ࡔ࠲ࡁ࡯࡞ 6ml ࠍട߃ߡṁ߆ߒ㧘0.1 㧑㈶㉄ 12ml ࠍട߃ߚ㧚ߎߩṁᶧࠍߘࠇߙࠇ 10ml ߩࡔ࠲㧞࡮㧟࡮㧞ࡁ࡯࡞㧘᳓෸߮ 0.1 㧑㈶㉄ࠍᵹߒߡࠦࡦ࠺࡚ࠪ࠾ࡦࠣߒ

ࡒࡦߩ HPLC ᷹ቯ᧦ઙ

ߚ Varian ⵾ߩ Mega Bond Elut SCX ࠞ ࡯࠻࡝࠶ࠫ㧔5g,

ࠞ࡜ࡓ

LiChrosorb RP18 (4.6×150mm, 5 Ǵ m)

20ml㧕 ߦᵹߒㄟߺ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦࠍ଻ᜬ

ࠞ࡜ࡓ᷷ᐲ

45 ͠

ߐߖߚ㧚ࠞ࡯࠻࡝࠶ࠫࠍ᳓ 10ml㧘ࡔ࠲ࡁ࡯࡞ 30ml ߢᵞ⒖േ⋧

pH7 ߩ 0.02mol/L ࡝ ࡦ㉄Ⴎ✭ⴣᶧ㧛ࡔ࠲ߞߚᓟ㧘4 㧑ࠕࡦࡕ࠾ࠕ᳓-ࡔ࠲ࡁ࡯࡞ṁᶧ 30ml ߢ⣕㧺⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜

ࡁ࡯࡞㧔15:85㧘 v/v㧕㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦࠍṁ಴ߒߚ㧚ṁ಴ᶧߪỚ❗ੇ࿕

ᵹ㊂

1.2ml / ಽᓟ㧘ዋ㊂ߩࠛ࡯࠹࡞ߦṁ߆ߒࠈ⚕ࠍ↪޿ߡࠈㆊߒߚ㧚ߘᵈ౉㊂10 Ǵ lߩࠈᶧࠍỚ❗ੇ῎ߒߡ⟤⊛りḮ 64g ߆ࠄ 29.2mg ࠍᓧߚ 㧚

᷹ቯᵄ㐳

225nm

HPLC ߢ᷹ቯߒߚ⚿ᨐ㧘ࠢࡠࡑ࠻ࠣ࡜ࡓ਄ߦන৻ࡇ࡯ࠢ

଻ᜬᤨ㑆⣕㧺-ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ㧦5.6 ಽ߇ᓧࠄࠇߚߎߣ߆ࠄ㧘એᓟ㧘ੑᰴᮡḰຠߣߒߡᧄᮡḰຠࠪࡉ࠻࡜ࡒࡦ㧦12.5 ಽࠍ↪޿ߚ㧚⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦࠍ 10.0mg㧘 ࠪࡉ࠻࡜ࡒ

㧟⚿ᨐ෸߮⠨ኤ

ࡦႮ㉄Ⴎࠍ 11.9mg ⒊㊂ߒ㧘ࡔ࠲ࡁ࡯࡞ࠍട߃ߡ 50ml ߣ㧟࡮㧝ߒ಄ᥧᚲߦ଻ሽߒߚ㧚↪ᤨ㧘ࡔ࠲ࡁ࡯࡞ࠍട߃ߡᏗ㉼ߒߩ HPLC ᷹ቯ᧦ઙߩᬌ⸛

20 㧙 50 Ǵ g/ml ߩᮡḰṁᶧࠍ⺞⵾ߒߚ㧚⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦ

LiChrosorb RP18 ߩ ࠞ࡜ࡓࠍ↪޿ߡ⣕㧺㧙ࠫࡔ࠴࡞ࠪ
Elutࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦߩ HPLC ߩ⒖േ⋧ࠍ 2㨯3㨯2

Certify ࠞ࡯࠻࡝࠶ࠫ㧔LRC, 300mg, 10ml㧕 ෸߮ Megaߩ᷹ቯ᧦ઙߢห᭽ߦᬌ⸛ߒߚ㧔࿑ 2㧕㧚 0.6 㧑ࠡ㉄࡮ 0.5

Bond Elut SCX ࠞ ࡯࠻࡝࠶ࠫ㧔5g, 20ml㧕ࠍ↪޿ߚ㧚ࡔ㧑࠻࡝ࠛ࠴࡞ࠕࡒࡦṁᶧ㧛ࠕ࠮࠻࠾࠻࡝࡞㧔20:80, v/v㧕㧘

㓁ࠗࠝࡦ੤឵૕ࡒ࠾ࠞ࡜ࡓߪ Varian ⵾ߩ Bond 

pH7 ߩ 0.02mol/L ࡝ ࡦ㉄Ⴎ✭ⴣᶧ㧛ࡔ࠲ࡁ࡯࡞㧔15:85㧘

ߪᷝട㊂ߩߘࠇߙࠇ 0 㧑㧘1 㧙 2ml ߩṁ಴㊂ߪᷝട㊂ߩv/v㧕෸߮ 0.04mol/L ㈶ ㉄ࠕࡦࡕ࠾࠙ࡓ㧛ࡔ࠲ࡁ࡯࡞㧛࠻

1.1 ෸߮ 0 㧑 㧘2 㧙 3ml ߩṁ಴㊂ߪᷝട㊂ߩ 90.8 ෸߮ 91.3

࡝ࠛ࠴࡞ࠕࡒࡦ㧔 20:80:0.05, v/v㧕 ߩ 3 ⒳㘃ߩ⒖േ⋧޿㧑 㧘3 㧙 4ml ߩṁ಴㊂ߪᷝട㊂ߩ 6.0 ෸߮ 5.6 㧑 㧘4 㧙 5mlߕࠇ߽㧘⦟ᅢߥಽ㔌߇ᓧࠄࠇߚ㧚࠻࡝ࠛ࠴࡞ࠕࡒࡦࠍ૶

ߩṁ಴㊂ߪᷝട㊂ߩ 0 㧑ߢ޽ߞߚ㧚ߎߩߎߣ߆ࠄࠢ࡝࡯

↪ߒߚ⒖േ⋧ߪࠞ࡜ࡓߩᐔⴧൻߦᤨ㑆߇߆߆ࠆߚ߼㧘ᧄࡦࠕ࠶ࡊᠲ૞ᤨߦ߅޿ߡ㧘 2 㧑ࠕࡦࡕ࠾ࠕ᳓ -ࡔ࠲ࡁ࡯⹜㛎ᴺߢߪ 㧘࿑ 2b ߦ␜ߒߚ HPLC ࠢࡠࡑ࠻ࠣ࡜ࡓߩ pH7࡞ṁᶧ㊂ߪ 4ml ߣߒߚ㧚ታ⹜ᢱߩ᷹ቯᤨߦߪṁ಴ᶧࠍ

ߩ 0.02mol/L ࡝ ࡦ㉄Ⴎ✭ⴣᶧ㧛ࡔ࠲ࡁ࡯࡞ࠍ↪޿ߚ㧚⓸⚛ࠟࠬ᳇ᵹਅߢ 2ml એਅ߹ߢỚ❗ᓟ㧘ࡔ࠲ࡁ࡯࡞ࠍ
ട߃ 2ml ߦߒߚ㧚ᷝട࿁෼⹜㛎ߢᓧࠄࠇߚஜᐽ㘩ຠ㧭෸߮㧮ߩࠢ࡝࡯ࡦࠕ࠶ࡊ೨ߩࠢࡠࡑ࠻ࠣ࡜ࡓࠍ࿑ 3a ෸߮࿑ 3c ߦ␜ߔ㧚࿑ 3b ෸߮࿑ 3d ߦࠢ࡝࡯ࡦࠕ࠶ࡊᓟߩ
ࠢࡠࡑ࠻ࠣ࡜ࡓࠍ␜ߔ㧚ߎߩࠢ࡝࡯ࡦࠕ࠶ࡊᠲ૞ߦࠃߞ
ߡஜᐽ㘩ຠਛߩ౒ሽ‛⾰ࠍലᨐ⊛ߦ㒰෰ߔࠆߎߣ߇ߢ߈
ߚ㧚

࿑ 23 ⒳㘃ߩ⒖േ⋧㧧0.6 㧑ࠡ㉄࡮0.5 㧑࠻࡝ࠛ࠴࡞ࠕࡒ ࡦ ṁ ᶧ 㧛 ࠕ ࠮ ࠻ ࠾ ࠻ ࡝ ࡞ 㧔 20:80㧕㧔 a㧕㧘 pH7 ߩ
0.02mol/L ࡝ ࡦ㉄Ⴎ✭ⴣᶧ㧛ࡔ࠲ࡁ࡯࡞㧔15:85㧕㧔b㧕㧘
0.04mol/L ㈶ ㉄ࠕࡦࡕ࠾࠙ࡓ㧛ࡔ࠲ࡁ࡯࡞㧛࠻࡝ࠛ࠴࡞
ࠕࡒࡦ㧔20:80:0.05㧕㧔c㧕ߢ᷹ቯߒߚᮡḰṁᶧ㧔ฦ‫ ޘ‬250ng㧕

࿑ 3

ߩ HPLC ࠢࡠࡑ࠻ࠣ࡜ࡓ

ࠕ࠶ࡊᓟ㧔b㧕෸߮ஜᐽ㘩ຠ㧮ߩࠢ࡝࡯ࡦࠕ࠶ࡊ೨㧔c㧕㧝㧘⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ㧧ஜᐽ㘩ຠ㧭ߩࠢ࡝࡯ࡦࠕ࠶ࡊ೨㧔 a㧕ߣࠢ࡝࡯ࡦ

ߣࠢ࡝࡯ࡦࠕ࠶ࡊᓟ㧔d㧕ߩ HPLC ࠢࡠࡑ࠻ࠣ࡜ࡓ

㧞㧘ࠪࡉ࠻࡜ࡒࡦ

㧝㧘⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ㧧㧟࡮㧞㧞㧘ࠪࡉ࠻࡜ࡒࡦ

౒ሽ‛⾰ߩ㒰෰⹜ᢱ᛽಴ᶧਛߩ౒ሽ‛⾰ࠍ Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫࠍ↪޿ߡࠢ࡝࡯ࡦࠕ࠶ࡊߔࠆᣇᴺࠍᬌ⸛ߒߚ㧚⣕㧺㧟࡮㧟⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦ

ߩ࿁෼₸ߣౣ⃻ᕈ㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦߘࠇߙࠇ 40

Ꮢ⽼ߐࠇߡ޿ࠆஜᐽ㘩ຠ㧭෸߮㧮㧔⚊⼺⩶ࠍ฽᦭ߔࠆ

Ǵ g ࠍ Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫߦ଻ᜬߐߖ㧘 2 㧑࠳ࠗࠛ࠶࠻↪ஜᐽ㘩ຠ 㧕ࠍ↪޿ߡᷝട࿁෼ታ㛎ࠍⴕߞߚ㧚ࠕࡦࡕ࠾ࠕ᳓ -ࡔ࠲ࡁ࡯࡞ṁᶧߩṁ಴㊂ࠍᬌ⸛ߒߚ㧚ߘஜᐽ㘩ຠ 0.2g ߦ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ

ߩ⚿ᨐ㧘2 㧑ࠕࡦࡕ࠾ࠕ᳓-ࡔ࠲ࡁ࡯࡞ṁᶧ 0 㧙 1ml ߢ࠻࡜ࡒࡦࠍߘࠇߙࠇ 50 Ǵ g ᷝടߒታ㛎ᠲ૞ߦᓥߞߡ࿁

ߪ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦṁ಴㊂෼₸ࠍ᳞߼ߚ 㧚➅ࠅ㄰ߒታ㛎ߪ 5 ࿁ⴕ޿㧘ߘߩ⚿ᨐࠍ⴫ 1 

Certify ࠞ ࡯࠻࡝࠶ࠫࠍ↪޿ࠆߎߣߦࠃࠅ౒ሽ‛⾰ࠍലߦ␜ߔ㧚ஜᐽ㘩ຠ㧭ߩ࿁෼₸ߪ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ߇
96.4 㧑㧔89.8 㧙 99.5 㧑 㧕㧘ࠪࡉ࠻࡜ࡒࡦ߇ 91.4 㧑㧔84.2

ᨐ⊛ߦ㒰෰ߔࠆߎߣ߇ߢ߈ߚ㧚߹ߚ㧘࿁෼₸㧘ౣ⃻ᕈߣ߽ߦ߶߷ḩ⿷ߔࠆ⚿ᨐࠍᓧߚ㧚㧙 95.7 㧑㧕ߢ޽ߞߚ㧚ஜᐽ㘩ຠ㧮ߩ࿁෼₸ߪ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ߇ 88.4 㧑㧔83.1 㧙 91.1 㧑 㧕㧘ࠪࡉ࠻࡜ࡒࡦ߇ 87.4 㧑㧔83.7 㧙 94.0 㧑㧕ߢ޽ߞߚ㧚ᄌേଥᢙߪ޿ߕࠇ߽ 5 㧑એౝߢ޽ࠅ߶߷ḩ⿷ߔࠆ⚿ᨐࠍᓧߚ㧚ᧄ
ᴺߦࠃࠆ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦ
ቯ㊂ਅ㒢୯ߪߘࠇߙࠇ 0.001mg/g ߢ ޽ߞߚ㧚
⴫1ஜᐽ㘩ຠ߆ࠄߩ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ
෸߮ࠪࡉ࠻࡜ࡒࡦߩᷝട࿁෼⹜㛎⚿ᨐ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ

ࠪࡉ࠻࡜ࡒࡦ

࿁෼₸ ± ᮡḰ஍Ꮕ**ஜᐽ㘩ຠ㧭*

96.4 ± 3.9

91.4 ± 4.5

ஜᐽ㘩ຠ㧮*

88.4 ± 4.8

87.4 ± 4.2

࿑ 4⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦ߇

ᬌ಴ߐࠇߚΌஜ⟤ੱ㧔a㧕෸߮シり⟤ੱ㧔b㧕ߩ HPLC ࠢ* ஜᐽ㘩ຠ A ෸߮ B ߦ߅޿ߡ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜

ࡠࡑ࠻ࠣ࡜ࡓ

ࡒࡦߪਇᬌ಴ߢ޽ߞߚ㧚㧝㧘⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ㧧㧞㧘ࠪࡉ࠻࡜ࡒࡦ

** ஜᐽ㘩ຠ 0.2g ߦ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻࡜ࡒࡦࠍߘࠇ

ᢥ₂

ߙࠇ 50 Ǵ g ᷝടߒߚ㧚ታ㛎࿁ᢙ㧔 n=5㧕- 

ᧄᴺࠍ⚊⼺⩶࠳ࠗࠛ࠶࠻ߣ⒓ߒߡࠗࡦ࠲࡯ࡀ࠶࠻⽼ᄁ
ߐࠇߚ 6 ⒳㘃ߩஜᐽ㘩ຠߦᔕ↪ߒߚ⚿ᨐ㧘⣕㧺㧙ࠫࡔ࠴C㧚 Gundlah et al : J Pharmacol Exp Ther 

F McMahon et al : Arch Intern Med - 

࡞ࠪࡉ࠻࡜ࡒࡦ޽ࠆ޿ߪࠪࡉ࠻࡜ࡒࡦߩ޿ߕࠇ߆߇ᬌ಴
ߐࠇߚ㧚6 ⒳㘃ߩ HPLC ࠢࡠࡑ࠻ࠣ࡜ࡓߪ࿑a ෸߮࿑b

A Ruiz et al : Obes Res  - 

ߦ␜ߔΌஜ⟤ੱ޽ࠆ޿ߪシり⟤ੱߩࠢࡠࡑ࠻ࠣ࡜ࡓߣ㘃



R Berkowitz et al : JAMA  - 

ૃߒߡ޿ߚ㧚⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦߩ฽᦭㊂ߪΌᷓᎹశม࡮ဈ↰೑ኅ㧦ᣣ⮎ℂ⹹㧘 -ஜ⟤ੱ㧘⟤⊛りḮ㧘⟤ᆫቲ෸߮ࠬ࡝ࡓ࠺࡞ࡊࡠ߇ߘࠇߙ
ࠇ 1.1㧘 0.7㧘 1.1 ෸߮ 1.3mg/g ߢ޽ߞߚ㧚ࠪࡉ࠻࡜ࡒࡦߩ





฽ ᦭ ㊂ ߪ シ り ⟤ ੱ ෸ ߮ ⾆ ቲ ⟤ ஜ ߇ ߘ ࠇ ߙ ࠇ 1.7 ෸ ߮


2.0mg/g ߢ޽ߞߚ㧚J Chen et al : J Chromatogr B  -ෘ↢ഭ௛⋭ක⮎㘩ຠዪ㧘⮎㘩⋙㤗⊒╙ภ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ



㧠㧚߹ߣ߼
ஜᐽ㘩ຠਛߩ⣕㧺㧙ࠫࡔ࠴࡞ࠪࡉ࠻࡜ࡒࡦ෸߮ࠪࡉ࠻
࡜ࡒࡦߩ HPLC ಽᨆᴺࠍᬌ⸛ߒߚ 㧚ߘߩ⚿ᨐ㧘Bond Elut ෘ↢ഭ௛⋭ක⮎㘩ຠዪ㧘ක⮎⋙㤗⊒╙ภ㧘╙ภࠪࡉ࠻࡜ࡒࡦ

Analysis of Des-N-dimethylsibutramine and Sibutramine in Health Foods by HPLC
Kunimasa MORITA, Takami MOHRI, Reiko NAKAGAWA
Fukuoka Institute of Health and Environmental Sciences,
Mukaizano 39, Dazaifu, Fukuoka 818-0135, JapanA reverse-phase HPLC method for determining des-N-dimethylsibutramine and sibutramine in health foods is described.
Des-N-dimethylsibutramine and sibutramine in health foods was extracted with methanol. The sample solution for HPLC wasapplied to Bond Elut Certify (solid phase extractions) with 2% ammonium hydroxide in methanol as the mobile phase. The
HPLC analysis was carried out on a column of LiChrosorb RP18 (4.6 x 150 mm, 5 microns) with 0.02 mol/L phosphate buffer
pH 7.0 /methanol (15:85, v/v) as the mobile phase and the eluate was monitored by UV detection at 225 nm. The average
recovery of des-N-dimethylsibutramine and sibutramine from health food was 㧙 and 㧙%, respectively. The
quantitation limit of this method was 0.001mg/g, respectively.
[Key words; des-N-dimethylsibutramine, Sibutramine, HPLC㧘Solid phase extractions, Health Food]  

⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ╙ภ㧘㧙㧘ේ⪺⺰ᢥஜᐽ㘩ຠਛߩࡆࠨࠦࠫ࡞ߩ HPLC ಽᨆᴺ᫪↰㇌ᱜ㧘Ძ೑㓉⟤㧘ਛᎹ␞ሶㅒ⋧♽ߩ㜞ㅦᶧ૕ࠢࡠࡑ࠻ࠣ࡜ࡈࠍ૶ߞߡ㧘ஜᐽ㘩ຠਛߩࡆࠨࠦࠫ࡞ߩಽᨆᴺࠍᬌ⸛ߒߚ㧚ஜᐽ㘩ຠਛߩࡆࠨࠦࠫ࡞ߪ 0.1 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ࠍ↪޿ߡ᛽಴ߒ㧘Bond Elut C18 ෸߮ Bond Elut Certify ࠞ࡯࠻࡝࠶ࠫ(࿕⋧᛽಴ᴺ)ࠍ↪޿ߡߘࠇߙࠇ 0.1 㧑㈶㉄ࡔ࠲ࡁ࡯࡞෸߮ 2 㧑ࠕࡦࡕ࠾ࠕ᳓-ࡔ࠲ࡁ࡯࡞ṁᶧߢࠢ࡝࡯ࡦࠕ࠶ࡊߒߚ㧚㜞ㅦᶧ૕ࠢࡠࡑ࠻ࠣ࡜ࡈߪࠞ࡜ࡓߦ LiChrosorb RP18 (4.6 x 150 mm, 5 microns)ࠍ㧘⒖
േ⋧ߦ pH7 ߩ 0.02 mol/L ࡝ࡦ㉄Ⴎ✭ⴣᶧ㧛ࠕ࠮࠻࠾࠻࡝࡞㧔55:45㧘v/v㧕ࠍ↪޿㧘᷹ቯᵄ㐳 260 nm ߢಽᨆߒߚ㧚ᧄᴺߦࠃࠆஜᐽ㘩ຠ߆ࠄߩࡆࠨࠦࠫ࡞ߩ࿁෼₸ߪ 73 㧙 97 㧑㧘ቯ㊂ਅ㒢୯ߪ 0.02 mg/g ߢ޽ߞߚ㧚㨇ࠠ࡯ࡢ࡯࠼ : ࡆࠨࠦࠫ࡞㧘HPLC㧘࿕⋧᛽಴ᴺ㧘ஜᐽ㘩ຠ㧘ਅ೷㨉㧝ߪߓ߼ߦ㐷ㇱ∩ߥߤߩ∝⁁߇⃻ࠇࠆߎߣ߇޽ࠆ

1),

2)

㧚

ᐔᚑ 14 ᐕ 12 ᦬㧙ᐔᚑ 17 ᐕ 9 ᦬㧘࠲ࠗ߆ࠄ୘ੱャ౉ߦࠃࠅ౉ᚻߒߚ‫ߣߤߥޠ࠻࠶ࠛࠗ࠳࡞࠲ࡇࠬࡎޟ‬⒓ߐࠇ
ࠆ⵾ຠࠍ᦯↪ߒߚߎߣߦࠃࠆஜᐽⵍኂ 4 ଀㧔ౝ㧝଀߇ᕆᕈᔃਇోߢᱫ੢㧕߇ෘ↢ഭ௛⋭߆ࠄ⊒⴫ߐࠇߚ㧚⹥ᒰຠߦߪࡆࠨࠦࠫ࡞ࠍߪߓ߼ߣߔࠆ 4 㧙 7 ⒳㘃ߩήᛚ⹺ή⸵

4,4'-(2-㩕㩩㩢㩆㩨㩣㩜㩋㩤㩧) 㩕㩨㩇㩖㨴㩓㨺㩣㧔desacetyl bisacodyl㧕นක⮎ຠ߇ᷙ౉ߐࠇ㧘5 ⒳㘃ߩ⹥ᒰຠߦว⸘ 11 ᚑಽߩක⮎ຠ߇ᬌ಴ߐࠇߚ㧚ࡆࠨࠦࠫ࡞ߪ⊕⦡☳ᧃߢ᳖㈶㉄ߦṁߌ߿ߔߊ㧘ࠕ࠮࠻ࡦߦ߿߿ṁߌ߿ߔߊ㧘ࠛ࠲ࡁ࡯࡞෶ߪࠛ࡯࠹࡞ߦṁߌߦߊߊ㧘᳓ߦ߶ߣࠎߤṁߌߥ޿㧚ᏗႮ㉄ߦṁߌࠆ߇ㅦ߿߆ߦ ಽ ⸃ ߒ 㧘 4,4'-(2-㩕 㩩 㩢㩆 㩨㩣 㩜㩋㩤 㩧 )㩕 㩨 㩇㩖㨴 㩓㨺㩣 㧔 desacetyl

bisacodyl㧕 ෸߮ 4,4'-(2-㩕 㩩㩢㩆㩨㩣㩜㩋㩤㩧 )4,4'-(2-㩕㩩㩢㩆㩨㩣㩜㩋㩤㩧) 㩕㩨㩇㩖㨴㩓㨺㩣㩝㩓㨻㩈㩍㨺㩎㩕㩨㩇㩖㨴㩓㨺㩣㩝㩓㨻㩈㩍㨺㩎㧔 monoacetyl bisacodyl㧕

㧔monoacetyl bisacodyl㧕 ࠍ↢ᚑߔࠆߎߣ߇ᣣᧄ⮎ዪᣇߦ⸥タߐࠇߡ޿ࠆ

1)㧚ൻቇᑼߪࡆࠨࠦࠫ࡞߇ C22H19NO4

㧔m.w. 361.4㧕 ߢ޽ࠆ㧚ࡆࠨࠦࠫ࡞ߪထ೷↪✭ਅ⮎ߢ޽ࠆ㧚⚿⣺㧘⋥⣺☼⤑ߩ೽੤ᗵ␹⚻ᧃ┵ߦ૞↪ߒߡߗࠎേࠍ㜞߼㧘߹ߚ⣺☼⤑߳ࡆࠨࠦࠫ࡞ߩ⋥ធ૞↪ߦࠃࠅឃଢ෻኿ࠍೝỗߔࠆ㧚ߐࠄߦ⚿⣺⣧ౝ

࿑1ߦ߅ߌࠆ᳓ಽ߿㔚⸃⾰ߩๆ෼ࠍᛥ೙ߔࠆ㧚⋥⣺ထ೷ߣߔࠦࠫ࡞ߩൻቇ᭴ㅧᑼ

desacetyl bisacodyl㧘 monoacetyl bisacodyl ෸ ߮ࡆࠨ

ࠇ߫ 15 㧙 60 ಽએౝߦ૞↪߇⃻ࠇࠆ㧚ଢ⒁∝㧘ᶖൻ▤ᬌᩏᤨ෶ߪᚻⴚ೨ᓟߦ߅ߌࠆᶖൻ▤ౝኈ‛ߩឃ㒰ߦㆡᔕߐࡆࠨࠦࠫ࡞ߩ᷹ቯᣇᴺߣߒߡㅒ⋧ࠞ࡜ࡓߣ⒖േ⋧ߦࠇࠆ㧚ࡆࠨࠦࠫ࡞ߪ 1 ࿁ 10 mg㧘 1 ᣣ 1 㧙 2 ࿁⢂㐷ౝߦ

0.01 mol/L ࠢ ࠛ ࡦ ㉄ 㧛 ࠕ ࠮ ࠻ ࠾ ࠻ ࡝ ࡞ 㧛 ࡔ ࠲ ࡁ ࡯ ࡞

ᝌ౉ߔࠆ

1)

㧚ࡆࠨࠦࠫ࡞ߪ 1 ୘ 2 mg ෸߮ 10 mg ฽߹2)

㧔20:10:10, v/v㧕1), 3)

㧘 0.05 mol/L ࡝ ࡦ㉄ੑ᳓⚛ࠞ࡝࠙ࡓ4)ࠇࠆထ⮎߇⽼ᄁߐࠇߡ޿ࠆ߇ 㧘⢈ḩߩᴦ≮ߩߚ߼ߦ૶

㧛ࠕ࠮࠻࠾࠻࡝࡞㧔45:55, v/v㧕 ࠍ↪޿ߚ HPLC ᴺ߇ႎ↪ߐࠇࠆක⮎ຠߢߪߥ޿㧚ࡆࠨࠦࠫ࡞ߩ೽૞↪ߣߒߡ㧘๔ߐࠇߡ޿ࠆ㧚ㄭᐕ㧘࿖᳃ߩஜᐽᔒะߦ઻ߞߡ޿ࠊࠁࠆ

⋥⣺ೝỗᗵ㧘⋥⣺Ἳ㧘߹ߚߣ߈ߦ⣻ㇱਇᔟᗵ㧘⣻∩㧘⢂ஜᐽ㘩ຠߦක⮎ຠ߇ᷝടߐࠇߚ߽ߩ߇㧘ᐫ㗡߿ㅢା⽼ᄁ⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲ㧔‫ ޥ‬ᄥቿᐭᏒᄢሼะ૒㊁㧕 ߢᵹㅢߔࠆࠃ߁ߦߥߞߚ㧚ߎߩࠃ߁ߥήᛚ⹺ή⸵นක⮎

㧑㈶㉄ࡔ࠲ࡁ࡯࡞ጀࠍណขߒߚ㧚ᱷᷲߪ 0.1 㧑㈶㉄ࡔ࠲ຠ޽ࠆ޿ߪஜᐽⵍኂ߇⊒↢ߔࠆ߅ߘࠇ߇޽ࠆ⵾ຠߦߟ޿

ࡁ࡯࡞ 2 ml ߢߐࠄߦ 1 ࿁᛽಴ߒߚ㧚᛽಴ᶧࠍวࠊߖ㧘0.1

ߡ㧘⪺⠪ࠄߪஜᐽⵍኂࠍᧂὼߦ㒐ߋߚ߼㧘ක⮎ຠ╬ߩᚑ

㧑㈶㉄ࠍട߃ߡ 10ml ߣߒߚ㧚Bond Elut Certify ࠞ ࡯࠻ಽᬌᩏࠍታᣉߒߡ޿ࠆ㧚࡝࠶ࠫߪࡔ࠲ࡁ࡯࡞ 2 ml㧘᳓ 2 ml㧘 0.1 㧑㈶㉄ 2 ml ࠍߎࠇ߹ߢߦႎ๔ߐࠇߚࡆࠨࠦࠫ࡞ߩ HPLC ಽᨆᴺ   㗅ᰴᵹߒࠦࡦ࠺࡚ࠪ࠾ࡦࠣߒߚ㧚10 ml ߩ⹜ᢱᶧࠍ Bondߪ⣺ṁᕈ㍤೷෸߮ထ⮎ߩ⹜㛎ᴺߢ޽ࠅ㧘ஜᐽ㘩ຠߩ⹜Elut Certify ࠞ ࡯࠻࡝࠶ࠫߦᵹߒㄟߺࡆࠨࠦࠫ࡞ࠍ଻ᜬ

㛎ᴺߢߪߥ޿㧚ᄙ⒳㘃ߩᅹኂᚑಽ߇ሽ࿷ߔࠆஜᐽ㘩ຠߦߐߖߚᓟ㧘᳓ 2 ml ෸߮ࡔ࠲ࡁ࡯࡞ 2 ml ߢᵞᵺߒߚ㧚ฃㆡ↪ߔࠆߦߪ♖⵾߇ᔅⷐߢ޽ࠆ㧚ߘߎߢ㧘࿕⋧᛽಴ᴺߢ

ེߦ 50 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ 1 ml ࠍ౉ࠇ㧘2 㧑ࠕࡦࡕ࠾ࠕ

ࠢ࡝࡯ࡦࠕ࠶ࡊߒ㧘HPLC ߢಽᨆߔࠆᣇᴺࠍᬌ⸛ߒߚ㧚᳓-ࡔ࠲ࡁ࡯࡞ṁᶧ 3 ml ߢࡆࠨࠦࠫ࡞ࠍṁ಴ߒ⹜ᢱṁᶧ



ߣߒߚ㧚
㧞

ታ㛎ᣇᴺ
㧞࡮㧟࡮㧞

ࡆࠨࠦࠫ࡞ߩ HPLC ᷹ቯ᧦ઙࡆࠨࠦࠫ࡞ᮡḰේᶧߪࡆࠨࠦࠫ࡞㧔Sigma ⵾ 㧕ࠍ 20 mg

ࠞ࡜ࡓLiChrosorb RP18 (4.6×150 mm, 5 Ǵ m)⒊㊂ߒ㧘0.1 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ࠍട߃ߡ 100 ml ߣߒ಄ᥧ⒖േ⋧pH7 ߩ 0.02 mol/L ࡝ ࡦ㉄Ⴎ✭ⴣᶧ㧛ࠕ࠮

㧞࡮㧝

⹜⮎

ᚲߦ଻ሽߒߚ㧚↪ᤨ㧘ᮡḰේᶧ 5࠻࠾࠻࡝࡞㧔55 : 45㧘 v/v㧕ml ࠍߣࠅ 0.1 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ࠍട߃ߡ 50 ml ߣߒߚ߽ߩࠍᮡḰṁᶧߣߒᵹ㊂

1.0 ml/min

ߚ㧚

ࠞ࡜ࡓ᷷ᐲ

45 ͠

࿕⋧᛽಴ߦߪ Varian ⵾ߩ Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫ

ᵈ౉㊂10 Ǵ l㧔LRC, 500 mg, 10 ml㧕 ෸߮㓁ࠗࠝࡦ੤឵૕ࡒ࠾ࠞ࡜ࡓ

᷹ቯᵄ㐳

260 nmߩ Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫ㧔LRC, 300 mg, 10 ml㧕଻ᜬᤨ㑆ࡆࠨࠦࠫ࡞㧦9.5 ಽࠍ↪޿ߚ㧚ࡔ࠲ࡁ࡯࡞ߪ๺శ⚐⮎Ꮏᬺ㧔ᩣ㧕⵾ߩ HPLC㧟ಽᨆ↪ࠍ↪޿ߚ㧚
2 㧑ࠕࡦࡕ࠾ࠕ᳓ -ࡔ࠲ࡁ࡯࡞ṁᶧߪࠕࡦࡕ࠾ࠕࠍ 2⚿ᨐ෸߮⠨ኤ

㧟࡮㧝ࡆࠨࠦࠫ࡞ߩಽ⸃ᕈ

㧑฽߻ࠕࡦࡕ࠾ࠕ᳓-ࡔ࠲ࡁ࡯࡞ṁᶧࠍ⺞⵾ߒߚ㧚㧞࡮㧞ⵝ⟎⚡ᄖๆశᐲᬌ಴ེ㧔 SPD-10AV㧕 ෸߮࠺࡯࠲ಣℂⵝ⟎㧔C-R3A㧕ઃ HPLC ߪፉᵤ⵾૞ᚲ㧔ᩣ㧕⵾ߩ LC10A ࠍ 㧘ࠞ࡜ࡓߪ GL ࠨࠗࠛࡦࠬ㧔ᩣ㧕⵾ߩ LiChrosorb

RP18

㧔4.6×150 mm, 5 Ǵ m㧕ࠍ૶↪ߒߚ㧚
㧞࡮㧟

ታ㛎ᠲ૞

㧞࡮㧟࡮㧝

ࡆࠨࠦࠫ࡞ߩಽᨆᴺ

Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫᴺ㧕ஜᐽ㘩ຠ 0.2 g ࠍ

10 ml ߩ⹜㛎▤ߦ⒊㊂ߒ㧘0.1 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ 2 ml ࠍ࿑ട߃ᝄߣ߁᛽಴ߒߚ㧚2500 rpm ߢ ㆙ᔃಽ㔌ߒߚᓟ㧘0.1

ࡆࠨࠦࠫ࡞ᮡḰṁᶧߩ HPLC ࠢࡠࡑ࠻ࠣ࡜ࡓ

㧑㈶㉄ࡔ࠲ࡁ࡯࡞ጀࠍណขߒߚ㧚ᱷᷲߪ 0.1 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ 2 ml ߢߐࠄߦ 1 ࿁᛽಴ߒߚ㧚᛽಴ᶧࠍวࠊߖ㧘࿑ 2 ߦ 2㨯3㨯2 ߩ᧦ઙߢ᷹ቯߒߚ 20 Ǵ g/ml ߩࡆࠨࠦࠫ

⓸⚛ࠟࠬ᳇ᵹਅ 40 㧙 50 ͠ߢ⚂ 1 ml ߹ߢỚ❗ߒ㧘0.1 㧑࡞ᮡḰṁᶧߩ HPLC ࠢ ࡠࡑ࠻ࠣ࡜ࡓࠍ␜ߔ㧚ࡆࠨࠦࠫ㈶㉄ࠍട߃ߡ 5 ml ߣߒߚ㧚Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫ

࡞ߪᏗႮ㉄ߦṁߌࠆ߇ㅦ߿߆ߦಽ⸃ߔࠆߣᣣᧄ⮎ዪᣇߦߪࡔ࠲ࡁ࡯࡞ 2 ml㧘᳓ 2 ml ࠍ㗅ᰴᵹߒࠦࡦ࠺࡚ࠪ࠾ࡦ

ႎ๔ߐࠇߡ޿ࠆ

ࠣߒߚ㧚5 ml ߩ⹜ᢱᶧࠍ Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫߦ

⹜ᢱ߆ࠄߩ᛽಴ߦㆡߒߚṁ೷ࠍㆬᛯߔࠆߚ߼㧘ᢙ⒳㘃ߩᵹߒㄟߺ㧘ࡆࠨࠦࠫ࡞ࠍ଻ᜬߐߖߚᓟ㧘0.1 㧑㈶㉄ 2 mlṁ೷ࠍ↪޿ߡࡆࠨࠦࠫ࡞Ớᐲ 20 Ǵ g/ml ߦ߅ߌࠆߘߩಽ

෸߮ 50 㧑ࡔ࠲ࡁ࡯࡞ 3ml ߢᵞᵺߒߚ㧚0.1 㧑㈶㉄ࡔ࠲⸃ᕈߦߟ޿ߡᬌ⸛ߒߚ㧚ࡁ࡯࡞ 5 ml ߢࡆࠨࠦࠫ࡞ࠍṁ಴ߒ⹜ᢱṁᶧߣߒߚ㧚
Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫᴺ㧕

1)㧚ࡆࠨࠦࠫ࡞ߩᮡḰṁᶧߩ⺞⵾෸߮ࡆࠨࠦࠫ࡞ 20 Ǵ g/ml ߩ 0.01 N Ⴎ㉄ࡔ࠲ࡁ࡯࡞ṁᶧஜᐽ㘩ຠ 0.2 gߢߪ㧘ᮡḰṁᶧ⺞⵾ 10 ಽᓟ㧘࿑ 3 ߦ␜ߔࡇ࡯ࠢ 3㧔଻ࠍ 10 ml ߩ⹜㛎▤ߦ⒊㊂ߒ㧘0.1 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ 2 mlᜬᤨ㑆 9.5 ಽ㧕ߩࡆࠨࠦࠫ࡞ߣ monoacetyl bisacodyl ߣ ⠨ࠍട߃ᝄߣ߁᛽಴ߒߚ㧚2500 rpm ߢ㆙ᔃಽ㔌ߒߚᓟ 㧘0.1

߃ࠄࠇࠆዊߐߥࡇ࡯ࠢ 2㧔଻ᜬᤨ㑆 4.7 ಽ㧕ߩ 2 ߟߩࡇ ࡯ࠢ߇ᬌ಴ߐࠇߚ㧚ߐࠄߦ 24 ᤨ㑆ᓟ㧔࿑ 3a㧕㧘desacetylࠇߚ㧚ߎߩߎߣ߆ࠄࡆࠨࠦࠫ࡞ߪႮ㉄㉄ᕈ෸߮ࠕࡦࡕ࠾

bisacodyl ߣ⠨߃ࠄࠇࠆࡇ࡯ࠢ 1㧔଻ᜬᤨ㑆 2.8 ಽ㧕߇ᬌࠕႮၮᕈߢߪᓢ‫ߦޘ‬ಽ⸃ߐࠇ㧘ᣣᧄ⮎ዪᣇߦ⸥タߐࠇߡ಴ߐࠇߚ㧚ࡆࠨࠦࠫ࡞ 20 Ǵ g/ml ߩࡔ࠲ࡁ࡯࡞෸߮ࠕ࠮

޿ࠆ monoacetyl bisacodyl ෸ ߮ desacetyl bisacodyl ߇ ↢ᚑ࠻࠾࠻࡝࡞ṁᶧߢߪ㧘ᮡḰṁᶧ⺞⵾ 6 ᤨ㑆ᓟࡆࠨࠦࠫ࡞

ߐࠇࠆߎߣ߇ಽ߆ߞߚ㧚ᧄ⹜㛎ᴺߢߪ㧘ᮡḰṁᶧߩṁ೷ߩߺ߇ᬌ಴ߐࠇߚ߇㧘ࡔ࠲ࡁ࡯࡞ṁᶧߢߪ 24 ᤨ㑆ᓟ㧔࿑෸߮᛽಴ṁ೷ߦߪᮡḰṁᶧ⺞⵾ᓟ㧝ࡩ᦬߹ߢಽ⸃ࡇ࡯ࠢ

3b㧕ዊߐߥࡇ࡯ࠢ 2 ߇ᬌ಴ߐࠇ㧘ߘߩᓟࡇ࡯ࠢ 2 ߪᣣ‫ޘ‬߇ోߊᬌ಴ߐࠇߥ߆ߞߚ 0.1 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ࠍ↪޿㜞ߊߥߞߚ㧚0.1 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ṁᶧߢߪ㧘࿑ 2 ߦ␜ߚ㧚ߒߚࠃ߁ߦ⺞⵾ᓟ㧝ࡩ᦬߹ߢࡇ࡯ࠢ 1 ෸߮ 2 ߩಽ⸃ࡇ࡯
ࠢߪోߊᬌ಴ߐࠇߥ߆ߞߚ㧚㧟࡮㧞౒ሽ‛⾰ߩ㒰෰

Bond Elut C18 ෸ ߮ Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫࠍ
↪޿ߡ⹜ᢱ᛽಴ᶧਛߩ౒ሽ‛⾰ࠍ㒰෰ߔࠆᣇᴺࠍᬌ⸛ߒߚ㧚ࡆࠨࠦࠫ࡞ 200 Ǵ g ࠍ Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫ
ߦ଻ᜬߐߖ㧘2㨯3㨯1 ߩᠲ૞ߦᓥߞߡ 0.1 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ߩṁ಴ᶧ㊂ࠍᬌ⸛ߒߚ㧚0 㧙 4

ml ߩṁ಴㊂ߪᷝട㊂ߩ⚂ 100 㧑ߢ޽ߞߚߎߣ߆ࠄ㧘ࠢ࡝࡯ࡦࠕ࠶ࡊᠲ૞ᤨߦ
߅ߌࠆ 0.1 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ߩṁ಴ᶧ㊂ߪ 5 ml ߣߒߚ 㧚ߐࠄߦ㧘ࡆࠨࠦࠫ࡞ 200 Ǵ g ࠍ Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫߦ଻ᜬߐߖ㧘2㨯3㨯1 ߩᠲ૞ߦᓥߞߡ 2 㧑ࠕࡦࡕ࠾ࠕ᳓-ࡔ࠲ࡁ࡯࡞ṁᶧߩṁ಴ᶧ㊂ࠍᬌ⸛ߒߚ 㧚0 㧙 2 mlߩṁ಴㊂ߪᷝട㊂ߩ⚂ 100 㧑ߢ޽ߞߚߎߣ߆ࠄ㧘ࠢ࡝࡯ࡦࠕ࠶ࡊᠲ૞ᤨߦ߅ߌࠆ 2 㧑ࠕࡦࡕ࠾ࠕ᳓ -ࡔ࠲ࡁ࡯࡞
ṁᶧߩṁ಴ᶧ㊂ߪ 3 ml ߣߒߚ㧚㪉㪌㩕㩨㩅㩄㩆㩨㩣 , ppm

㪉㪇
㪈㪌
㪈㪇
㪌
㪇
㪇

㪇㪅㪈

㪇㪅㪉

㪇㪅㪊

㪇㪅㪋

㪇㪅㪌

㪇㪅㪍

㪇㪅㪎

㪇㪅㪏

㈶㉄‫ޓ‬ml࿑ࡆࠨࠦࠫ࡞ 20 Ǵ g/ml ߩ 0.01 N Ⴎ㉄ࡔ࠲ࡁ࡯࡞ṁ࿑ᶧ㧔C 㧕㧘ࡔ࠲ࡁ࡯࡞ṁᶧ㧔D 㧕㧘 2 㧑ࠕࡦࡕ࠾ࠕ᳓ -ࡔ࠲ࡆࠨࠦࠫ࡞ߩಽ⸃ᕈ

ࡁ࡯࡞ṁᶧ㧔E㧕෸߮ 2 㧑ࠕࡦࡕ࠾ࠕ᳓-ࠕ࠮࠻࠾࠻࡝࡞

*2 㧑ࠕࡦࡕ࠾ࠕ᳓ -ࡔ࠲ࡁ࡯࡞ṁᶧ 3 ml ߦ㈶㉄ 0 㧙 0.8 ml ࠍട߃ߚᶧ

ṁᶧ㧔F㧕ߩ HPLC ࠢࡠࡑ࠻ࠣ࡜ࡓ

ߦ㧘ࡆࠨࠦࠫ࡞ࠍߘࠇߙࠇ 200 Ǵ g ࠍᷝടߒࡔ࠲ࡁ࡯࡞ߢ 10 ml ߣߒߚ*(a)෸߮ b)㧧 ᮡḰṁᶧ⺞⵾ᤨ㑆ᓟ᷹ቯ , (c)෸߮ F 㧧 ᮡḰṁᶧ⺞⵾ 10ṁᶧࠍ⺞⵾ߒߚ 㧚6 ᤨ㑆⚻ㆊᓟ 㧘ࡆࠨࠦࠫ࡞Ớᐲࠍ᷹ቯߒߚ㧚ታ㛎࿁ᢙ 1

ಽᓟ᷹ቯ

࿁

ࡇ࡯ࠢ㧧 desacetyl bisacodyl㧘ࡇ࡯ࠢ㧧monoacetyl bisacodyl㧘ࡇ࡯ࠢ㧧ࡆࠨࠦࠫ࡞

ߒ߆ߒ㧘 2 㧑ࠕࡦࡕ࠾ࠕ᳓ -ࡔ࠲ࡁ࡯࡞ṁᶧࠍṁ಴ᶧႮၮᕈṁᶧߢߩಽ⸃ᕈߦߟ޿ߡᬌ⸛ߒߚ⚿ᨐ㧘ࡆࠨࠦߦ↪޿ࠆߎߣߦࠃࠅ㧘HPLC ࠢࡠࡑ࠻ࠣ࡜ࡓߦ࿑ 3 ߦ␜ࠫ࡞ 20 Ǵ g/ml ߩ 2 㧑ࠕࡦࡕ࠾ࠕ᳓-ࡔ࠲ࡁ࡯࡞ṁᶧߢߒߚࡇ࡯ࠢ 1 ෸߮ 2 ߩಽ⸃↢ᚑ‛߇಴⃻ߒߚ㧚ߘߎߢ㧘ߪ㧘ᮡḰṁᶧ⺞⵾ 10 ಽᓟ㧘ࡇ࡯ࠢ 1 ෸߮ 2 ߇ᬌ಴ߐࠇ

ࡆࠨࠦࠫ࡞ߩಽ⸃ࠍᛥ೙ߔࠆߚ߼ฃེߦ޽ࠄ߆ߓ߼౉ࠇ

ߚ㧔࿑ 3c㧕㧚2 㧑ࠕࡦࡕ࠾ࠕ᳓-ࠕ࠮࠻࠾࠻࡝࡞ṁᶧߢߪ 㧘ߡ⟎ߊ㈶㉄㊂ߦߟ޿ߡᬌ⸛ߒߚ㧚 2 㧑ࠕࡦࡕ࠾ࠕ᳓ -ࡔ

10 ಽ⚻ㆊᓟዊߐߥࡇ࡯ࠢ 2 ߇ᬌ಴ߐࠇ㧔࿑ 3d㧕㧘ߐࠄߦ࠲ࡁ࡯࡞ṁᶧ 3 ml ߣ㈶㉄ 0 㧙 0.8 ml ߩᷙวṁᶧߦࡆࠨ24 ᤨ㑆ᓟ࿑ 3c ߣ㘃ૃߒߚ HPLC ࠢࡠࡑ࠻ࠣ࡜ࡓ߇ᓧࠄ

ࠦࠫ࡞ࠍߘࠇߙࠇ 200 Ǵ g ᷝടߒ㧘ࡔ࠲ࡁ࡯࡞ࠍട߃ߡ 

10 ml ߣߒߚ 20 Ǵ g/ml ߩࡆࠨࠦࠫ࡞ṁᶧࠍ⺞⵾ߒߚ㧚ᮡḰṁᶧ⺞⵾ 6 ᤨ㑆⚻ㆊᓟ HPLC ߢࡆࠨࠦࠫ࡞Ớᐲࠍ᷹ቯߒߚ⚿ᨐ㧔࿑ 4㧕㧘 2 㧑ࠕࡦࡕ࠾ࠕ᳓-ࡔ࠲ࡁ࡯࡞ṁᶧ 3 ml ߦኻߒߡ㈶㉄ 0 㧙 0.05 ml ߩᷙวṁᶧߢߪ㧘ࡆࠨࠦࠫ࡞ߪ 0 Ǵ g/ml ߢ࿑ 3 ߦ␜ߒߚࡇ࡯ࠢ 1 ߩߺ߇ᬌ಴
ߐࠇ㧘ࡆࠨࠦࠫ࡞ߪߔߴߡಽ⸃ߐࠇߡ޿ߚ㧚㈶㉄ 0.1 㧙
0.2 ml ߩᷙวṁᶧߢߪ㧘ࡆࠨࠦࠫ࡞Ớᐲߪ 1.8 㧙 15.1 Ǵ
g/ml ߢ࿑ 3 ߦ␜ߒߚࡇ࡯ࠢ 1 ෸߮ 2 ߩಽ⸃↢ᚑ‛߇ᬌ಴ߐࠇߚ㧚㈶㉄ 0.4 㧙 0.8 ml ߩᷙวṁᶧߢߪ㧘ࡆࠨࠦࠫ࡞Ớᐲߪ 20 Ǵ g/ml ߢ㧘ࡆࠨࠦࠫ࡞ߪోߊಽ⸃ߐࠇߥ߆
ߞߚ㧚ᧄ⹜㛎ᴺߢߪ㧘ฃེߦ౉ࠇࠆ㈶㉄㊂ߪ 0.5 ml㧘ߔߥࠊߜ 50 㧑㈶㉄ࡔ࠲ࡁ࡯࡞ 1 ml ߣߒߚ㧚
࿑ 5 ߦᷝട࿁෼⹜㛎ߢᓧࠄࠇߚஜᐽ㘩ຠ㧭෸߮㧮ߩࠢ࡝࡯ࡦࠕ࠶ࡊ೨ᓟߩ HPLC ࠢ ࡠࡑ࠻ࠣ࡜ࡓࠍ␜ߔ㧚ஜᐽ㘩ຠ㧭ߩ႐ว 㧘ࠢ࡝࡯ࡦࠕ࠶ࡊ೨ߩࠢࡠࡑ࠻ࠣ࡜ࡓ㧔࿑5a㧕ߪ౒ሽ‛⾰ߩᓇ㗀ࠍฃߌࡆࠨࠦࠫ࡞ߩࡇ࡯ࠢߦ࠹࡯
࡝ࡦࠣ߇⹺߼ࠄࠇߚ߇㧘Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫߢ
ಣℂߒߚᓟ㧔࿑ 5b㧕߆ߥࠅᡷༀߐࠇߚ㧚Bond Elut Certifyࠞ࡯࠻࡝࠶ࠫߢಣℂߒߚ႐ว㧔࿑ 5c㧕 Bond Elut C18 ࠞ࡯࠻࡝࠶ࠫࠃࠅߐࠄߦലᨐ⊛ߦ౒ሽ‛⾰ࠍ㒰෰ߔࠆߎߣ
߇ߢ߈ߚ߇㧘ࠢࡠࡑ࠻ࠣ࡜ࡓߦࡇ࡯ࠢ 1 ෸߮ 2 ߩಽ⸃↢ᚑ‛߇⹺߼ࠄࠇߚ 㧚ߎࠇࠄߩࡇ࡯ࠢߪ 2 㧑ࠕࡦࡕ࠾ࠕ᳓ࡔ࠲ࡁ࡯࡞ṁ಴ᶧ߇ Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫࠍㅢ
ㆊߔࠆ㓙㧘ࡆࠨࠦࠫ࡞߇ಽ⸃ߒ↢ᚑߒߚ߽ߩߣ⠨߃ࠄࠇߚ㧚ஜᐽ㘩ຠ㧮ߩ႐ว㧘ࠢ࡝࡯ࡦࠕ࠶ࡊ೨ߩࠢࡠࡑ࠻ࠣ
࡜ࡓ㧔࿑ 5d㧕ߪ଻ᜬᤨ㑆 1 㧙 6 ಽߦᄢ߈ߥࡇ࡯ࠢ߇޽
ࠆ߇㧘ࡆࠨࠦࠫ࡞ߩࡇ࡯ࠢߦᭂ┵ߥ࠹࡯࡝ࡦࠣ߇ߺࠄࠇ
ߥ޿ߚ߼㧘ࠞ࡯࠻࡝࠶ࠫᧂಣℂߢቯ㊂ߢ߈ࠆ⹜ᢱߣ⠨߃
ࠄࠇߚ㧚Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫಣℂᓟ㧔࿑ 5e㧕ߦ߅޿ߡ߽଻ᜬᤨ㑆 1 㧙 6 ಽߩ౒ሽ‛⾰ߪ㒰෰ߢ߈ߥ߆ߞ
ߚ߇ 㧘Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫߢಣℂߒߚ႐ว㧔࿑
5f㧕౒ሽ‛⾰ࠍലᨐ⊛ߦ㒰෰ߔࠆߎߣ߇ߢ߈ߚ㧚ߒ߆ߒ㧘ஜᐽ㘩ຠ㧭ߩ႐วߣห᭽ߦࡇ࡯ࠢ 1 ෸߮ 2 ߩಽ⸃↢ᚑ‛߇⹺߼ࠄࠇߚ㧚౒ሽ‛⾰ߩ㒰෰ࠍᬌ⸛ߔࠆߚ߼ߦ↪޿ߚஜᐽ㘩ຠ㧭෸߮㧮ߩࠢࡠࡑ࠻ࠣ࡜ࡓ਄ߦ㧘ࡆࠨࠦࠫ࡞ߩ
ಽᨆࠍᅹኂߔࠆࠃ߁ߥᚑಽߪ⹺߼ࠄࠇߥ߆ߞߚ߇㧘߽ߒࡆࠨࠦࠫ࡞ߩ଻ᜬᤨ㑆೨ᓟߦ౒ሽ‛⾰߇ሽ࿷ߔࠆஜᐽ㘩ຠ߇޽ࠇ߫㧘࿑ 5a ෸߮࿑ 5d ߦ␜ߒߚࠃ߁ߦ౒ሽ‛⾰ࠍ࿑ஜᐽ㘩ຠ㧭෸߮㧮ߩࠢ࡝࡯ࡦࠕ࠶ࡊ೨ᓟߩ HPLC

߶ߣࠎߤ㒰෰ߢ߈ߚ Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫࠍ↪

ࠢࡠࡑ࠻ࠣ࡜ࡓ

޿ߚᣇ߇⦟޿ߣᕁࠊࠇߚ㧚߹ߚ Bond Elut Certify ࠞ ࡯࠻* ஜᐽ㘩ຠ㧭ߩࠢ࡝࡯ࡦࠕ࠶ࡊ೨㧔C㧕㧘 Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫಣℂ

࡝࠶ࠫߢಣℂߒߚᓟߩಽ⸃↢ᚑ‛ߩࡇ࡯ࠢߩ᦭ήߪࡆࠨ

ᓟ㧔D㧕෸߮ Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫಣℂᓟ㧔E㧕㧚

ࠦࠫ࡞ߩ⏕⹺⹜㛎ߣߒߡ૶↪ߢ߈ࠆߣᕁࠊࠇߚ㧚** ஜᐽ㘩ຠ㧮ߩࠢ࡝࡯ࡦࠕ࠶ࡊ೨㧔F㧕㧘Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫಣ㧟࡮㧟ℂᓟ㧔G㧕෸߮ Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫಣℂᓟ㧔H㧕㧚

ࡆࠨࠦࠫ࡞

ࡆࠨࠦࠫ࡞ߩ࿁෼₸ߣౣ⃻ᕈᏒ⽼ߐࠇߡ޿ࠆஜᐽ㘩ຠ㧭෸߮㧮ࠍ↪޿ߡᷝട࿁෼⹜

ࡇ࡯ࠢ㧧 desacetyl bisacodyl㧘 ࡇ࡯ࠢ㧧monoacetyl bisacodyl㧘 ࡇ࡯ࠢ㧧㛎ࠍⴕߞߚ 㧚ஜᐽ㘩ຠ 0.2 g ߦࡆࠨࠦࠫ࡞ࠍߘࠇߙࠇ 200 Ǵ g ࠍᷝടߒታ㛎ᠲ૞ߦᓥߞߡ࿁෼₸ࠍ᳞߼ߚ㧚➅ࠅ㄰࡯ࡦࠕ࠶ࡊಣℂ೨ߩࡆࠨࠦࠫ࡞ߩ࿁෼₸ߪ 99.9 㧑㧔 99.0ߒታ㛎ߪ 5 ࿁ⴕ޿㧘ߘߩ⚿ᨐࠍ⴫㧝ߦ␜ߔ㧚

㧙 100.6 㧑 㧕㧘Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫಣℂᓟߩ࿁෼₸ߪ 97.4 㧑㧔96.7 㧙 98.6 㧑 㧕㧘 Bond Elut Certify ࠞ ࡯࠻⴫㧝࡝࠶ࠫಣℂᓟߩ࿁෼₸ߪ 73.0 㧑㧔70.7 㧙 76.5 㧑㧕ߢ޽ஜᐽ㘩ຠ߆ࠄߩࡆࠨࠦࠫ࡞ߩᷝട࿁෼⹜㛎⚿ᨐߞߚ㧚ᄌേଥᢙߪ޿ߕࠇ߽ 5 㧑એౝߢ޽ࠅ߶߷ḩ⿷ߔࠆࡆࠨࠦࠫ࡞⚿ᨐࠍᓧߚ㧚ᧄᴺߦࠃࠆࡆࠨࠦࠫ࡞ߩቯ㊂ਅ㒢୯ߪ 0.02

࿁෼₸ ± ᮡḰ஍Ꮕ**

mg/g ߢ޽ߞߚ㧚ࠢ࡝࡯ࡦࠕ࠶ࡊಣℂ೨ஜᐽ㘩ຠ㧭*

99.5 ± 0.9ஜᐽ㘩ຠ㧮*

99.9 ± 0.6㧠߹ߣ߼ஜᐽ㘩ຠਛߩࡆࠨࠦࠫ࡞ߩ HPLC ಽᨆᴺࠍᬌ⸛ߒߚ㧚ߘߩ⚿ᨐ㧘Bond Elut C18 ෸ ߮ Bond Elut Certify ࠞ ࡯࠻

Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫಣℂᓟஜᐽ㘩ຠ㧭

96.9 ± 0.4

࡝࠶ࠫࠍ↪޿ലᨐ⊛ߦ౒ሽ‛⾰ࠍ㒰෰ߔࠆߎߣ߇ߢ߈

ஜᐽ㘩ຠ㧮

97.4 ± 0.7ߚ㧚߹ߚ㧘࿁෼₸㧘ౣ⃻ᕈߣ߽ߦ߶߷ḩ⿷ߔࠆ⚿ᨐࠍᓧߚ㧚Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫಣℂᓟஜᐽ㘩ຠ㧭

92.3 ± 3.3

ஜᐽ㘩ຠ㧮

73.0 ± 2.3

ᢥ₂ዪᣇ⸃⺑ᦠ✬㓸ᆔຬળ✬㧕㧘%, ᧲੩㧘ᑝᎹ* ஜᐽ㘩ຠ㧭෸߮㧮ߦ߅޿ߡࡆࠨࠦࠫ࡞ߪਇᬌ಴ߢ޽ߞߚ㧚** ஜᐽ㘩ຠ 0.2㋈ᧁ㇚↢ࠄ㧦╙ 13 ᡷᱜᣣᧄ⮎ዪᣇ⸃⺑ᦠ㧘㧔ᣣᧄ⮎ᦠᐫ㧘㧚g ࠍណࠅ㧘ࡆࠨࠦࠫ࡞ࠍߘࠇߙࠇ 200 Ǵ g ᷝടߒߚ㧚ታ㛎࿁ᢙ㧔 n=5㧕㧚㐳የᷡࠄ㧦ᣣᧄක⮎ຠ㓸㧘㧔ᣣᧄක⮎ᖱႎ࠮ࡦ࠲࡯✬㧕㧘᧲੩ㇺ㧘⮎ᬺᤨႎ␠㧚

ஜᐽ㘩ຠ㧭ߩ႐ว㧘ࠢ࡝࡯ࡦࠕ࠶ࡊಣℂ೨ߩࡆࠨࠦࠫ

Chem  - 

࡞ߩ࿁෼₸ߪ 99.5 㧑㧔98.8 㧙 100.7 㧑 㧕㧘Bond Elut C18
ࠞ࡯࠻࡝࠶ࠫಣℂᓟߩ࿁෼₸ߪ 96.9 㧑㧔96.4 㧙 97.5 㧑 㧕㧘L Valenti & C Lau-Cam : J Assoc Off Anal

Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫಣℂᓟߩ࿁෼₸ߪ 92.3 㧑

K Bradshaw et al : J Pharm Biomed Anal - 

㧔 89.7 㧙 97.8 㧑㧕ߢ޽ߞߚ㧚ஜᐽ㘩ຠ㧮ߩ႐ว㧘ࠢ࡝

Analysis of Bisacodyl in Health Foods by HPLC
Kunimasa MORITA, Takami MOHRI, Reiko NAKAGAWA
Fukuoka Institute of Health and Environmental Sciences,
Mukaizano 39, Dazaifu, Fukuoka 818-0135, JapanA reverse-phase HPLC method for determining bisacodyl in health foods is described. Bisacodyl in health foods was
extracted with 0.1% acetic acid in methanol. The sample solution for HPLC was applied to Bond Elut C18 and Bond Elut
Certify (solid phase extractions) with 0.1% acetic acid in methanol and 2% ammonium hydroxide in methanol as the mobile
phase, respectively. The HPLC analysis was carried out on a column of LiChrosorb RP18 (4.6 x 150 mm, 5 microns) with 0.02
mol / L phosphate buffer pH 7.0 /acetonitrile (55:45, v/v) as the mobile phase and the eluate was monitored by UV detection at
260 nm. The average recovery of bisacodyl from health food was 73 㧙 97%. The quantitation limit of this method was 0.02
mg/g.
[Key words; Bisacodyl, HPLC㧘Solid phase extractions, Health Food, Cathartics]  

⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ╙ภ㧘㧘ේ⪺⺰ᢥᷧᵹ᳓ߩ࠻࡝ࡂࡠࡔ࠲ࡦ↢ᚑ⢻᳗ᷗ⟵ቁ㧖㧘᧻የብ㧖㧘૒‫ᧁޘ‬㊀ⴕ㧖⑔ጟ⋵ౝߩ⋵༡ᨋౝ߆ࠄᵹ಴ߔࠆᷧᵹ᳓ߩ࠻࡝ࡂࡠࡔ࠲ࡦ↢ᚑ⢻ߦߟ޿ߡᬌ⸛ߒߚ㧚⺞ᩏᵹၞߩ W ́㧘W ́ ෸߮ E ́ ߦ߅ߌࠆ࠻࡝ࡂࡠࡔ࠲ࡦ↢ᚑ⢻ߩᐔဋ୯ߪ㧘ߘࠇߙࠇ 㧘㧘 mg/Lߢ޽ߞߚ㧚࠻࡝ࡂࡠࡔ࠲ࡦ↢ᚑ⢻ߪቄ▵ᄌേ߇ߺࠄࠇߚ㧚࠻࡝ࡂࡠࡔ࠲ࡦ↢ᚑ⢻ߣਇ㘻๺⚿วࠍ᦭ߔࠆ
᦭ᯏ‛ߩᜰᮡߣߥࠆ⚡ᄖ✢ๆశᐲ㧔E㧕ߣߩ㑆ߦߪᱜߩ⋧㑐߇޽ߞߚ㧚W ́ ෸߮ E ́ ߦ߅ߌࠆ࠻
࡝ࡂࡠࡔ࠲ࡦ↢ᚑ⢻ߩᐕ㑆ᵹ಴⽶⩄㊂ߪ㧘́ g/ha ߢ޽ߞߚ㧚ާࠠ࡯ࡢ࡯࠼㧦ᷧᵹ᳓㧘࠻࡝ࡂࡠࡔ࠲ࡦ↢ᚑ⢻㧘࠻࡝ࡂࡠࡔ࠲ࡦ↢ᚑ⢻ᵹ಴⽶⩄ި㧝Ⴎ⚛ಣℂߒߚߣ߈ߦ↢ᚑߔࠆ THM Ớᐲߩߎߣߢ޽ࠆ㧚ߪߓ߼ߦ࠻࡝ࡂࡠ ࡔ࠲ࡦ 㧔 THM㧕 ߩ↢ ᚑߦ㑐ਈ ߔࠆ᦭ ᯏ‛ߪ㧘ὐ Ḯߣߐࠇࠆኅᐸ
㧟㧕㧝㧕߿Ꮏ႐࡮੐ᬺ႐㧘ਅ᳓㧝 㧕㧘 㧞 㧕એᓟߪ THMFP ߣ⇛ߔࠆ 㧚㧕ߩⷙ೙߇ⴕࠊࠇࠆߎߣߣ
ߥߞߚ㧚㕙Ḯߢߪ⊒↢Ḯ߇᣿⏕ߢߥ߆ߞߚࠅ㧘ߘߩᛠីߦ࿎

෸߮ߒዩಣℂ႐ 㧝㧕㧘㧠㧕޽ࠆ޿ߪ㕙Ḯߣߐࠇࠆ᫪ᨋ 㧝㧕㧘↰⇌ 㧘〝㕙ၸⓍ‛
㧝㧕

ߩ⮺㘃

㧡㧕㧘㧢㧕

㧟㧕

╬ߩᵹ಴᳓ߐࠄߦ᳓ਛߩ޽ࠆ⒳㔍ߐࠍ઻߁ߎߣ߇ᄙ޿㧚․ߦ᫪ᨋߦଥࠆ࠺࡯࠲ߩ෼㓸࡮⫾Ⓧ߇චಽߢߪߥߊ㧘 THMFP ⽶⩄㊂╬ߩ⷗Ⓧ߽ࠅߦ↱᧪ߒߡ޿ࠆ㧚᳓㆏ߩ᳓⾰ၮḰ㗄⋡ߦ߅޿ߡ THM ߩၮḰ୯ߪ

߇┙ߡߦߊ޿ߩ߇⃻⁁ߢ޽ࠆ㧚ߎߩߚ߼㧘 THMFP ߩ

mg/L ߣߥߞߡ߅ࠅ㧘᳓㆏᳓Ḯၞߢߪߎߩ᳓⾰ၮḰࠍࡃ࠶ࠢࠣ࡜࠙ࡦ࠼ߣ⠨߃ࠄࠇࠆ᫪ᨋၞ߆ࠄߩ࠺࡯࠲ࠍ

⿥ㆊߒߥ޿ࠃ߁ߥ᳓㆏ේ᳓᳓⾰଻ోኻ╷߇᳞߼ࠄࠇߡ

෼㓸࡮⫾Ⓧߔࠆߎߣߪ㧘᳓⾰଻ోኻ╷ࠍ⠨ᘦߔࠆ߁߃

޿ࠆ㧚ὐḮߢߪߘߩ⊒↢Ḯ߇᣿⏕ߦᛠីߢ߈ࠆߚ߼㧘ߢ㊀ⷐߣ⠨߃ࠄࠇࠆ㧚ᐔᚑ  ᐕ  ᦬ߦ‫․ޟ‬ቯ᳓㆏೑᳓㓚ኂ㒐ᱛߩߚ߼ߩ᳓

ߎߎߢߪ㧘⑔ጟ⋵ౝߩࠬࠡ࡮ࡅࡁࠠࠍਥߣߔࠆੱᎿ

㆏᳓Ḯ᳓ၞߩ᳓⾰଻ోߦ㑐ߔࠆ․೎ភ⟎ᴺ‫౏߇ޠ‬Ꮣߐᨋ߆ࠄᵹ಴ߔࠆᷧᵹ᳓ࠍኻ⽎ߦ⚂  ᐕ㑆ߦࠊߚߞߡ

ࠇ㧘ᜰቯ࿾ၞౝߢߪ৻ቯⷙᮨએ਄ߩ੐ᬺ႐ߦߟ޿ߡߪ㧘᳓⾰⺞ᩏࠍⴕ޿㧘᫪ᨋၞ↱᧪ߩ᦭ᯏ‛ߦ⿠࿃ߔࠆ

ឃ಴᳓ߩ࠻࡝ࡂࡠࡔ࠲ࡦ↢ᚑ⢻㧔⹜᳓ࠍ޽ࠆ᧦ઙਅߢ

THMFP ߩ࡟ࡌ࡞߿ᵹ಴⽶⩄㊂ࠍᬌ⸛ߒߚ㧚
㧞

⺞ᩏᣇᴺ

㧞࡮㧝

⺞ᩏᵹၞߣߘߩ᭎ⷐ

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⑔ጟ⋵ᄥቿᐭᏒᄢሼะ૒㊁㧕⑔ጟ⋵ਭ⇐☨Ꮢጊᧄ↸⼾↰́㧕 ⺞ᩏᵹၞߪࠬࠡߣࡅࡁࠠࠍਥߣߒߚੱᎿᨋ߆ࠄߥࠅ 㧘㧥ߦḰ᜚ߒߚ㧚⚡ᄖ✢ๆశᐲߪ㧘⹜᳓ࠍ µ O ߩࡔ

W ́ ߢߪ㧘ⶄጀᨋ㧔ਅᧁߩᨋ㦂ߪ  ᐕ㧘਄ᧁߪࡦࡉ࡜ࡦࡈࠖ࡞࠲࡯ߢࠈㆊߒߚࠈᶧߦߟ޿ߡ㧘 EO́ ᐕ㧕߇ᵹၞ㕙Ⓧߩ߶߷ඨಽߢ޽ࠆ  ha ࠍ 㧘࠮࡞ࠍ↪޿ߡๆశᐲ㧔ᵄ㐳  PO㧕ࠍ᷹ቯߒߚ㧚߹ߚ㧘 W ́ ߢߪ㧘ⶄጀᨋ㧔ਅᧁߩᨋ㦂ߪ  ᐕ㧘㧞࡮㧡ᵹ㊂෸߮ᵹ಴⽶⩄㊂╬ߩ▚ቯᣇᴺ਄ᧁߪ ́ ᐕ㧕߇ᵹၞ㕙Ⓧߩඨಽߢ޽ࠆ  haᵹ㊂ߦߟ޿ߡߪ⥄⸥᳓૏⸘ߩ᳓૏߆ࠄ⸘▚ߦࠃࠅ᳞ࠍභ߼ߡ޿ࠆ㧚৻ᣇ㧘 E ́ ߪ㧘 W ́ ෸߮ W ́߼ ߚ ߹ ߚ 㧘ᐕ㑆 ࡋ ࠢ࠲ ࡯࡞ 㧔 ha㧕޽ ߚ ࠅߩ THMFPߣߪ⇣ߥࠅ㧘නጀᨋ㧔ᨋ㦂ߪ ́ ᐕ㧕ߢ޽ࠆ㧚߹

ߩᵹ಴⽶⩄㊂ߩ⸘▚ߪᰴߩࠃ߁ߦߒߡⴕߞߚ㧚ߔߥࠊߚ㧘⺞ᩏᵹၞߩၮጤߪోߡ⚿᥏ߜ㧘ណ᳓ᣣᲤߩ THMFP㧔 C g/m  㧕ߣᵹ㊂㧔 Q m /ᣣ㧕㧞࡮㧞ጤߢ޽ࠆ 㧚
㧣㧕⺞ᩏᦼ㑆ߣࠍਸ਼ߓߡ THMFP ⽶⩄㊂㧔 L ᐕ  ᦬́ ᐕ  ᦬㧞࡮㧟g/ᣣ㧕ࠍ▚಴ߒߚ㧚ᰴߦ㧘 L ߣ Q ߣߩ㑐ଥߩᢔᏓ࿑ࠍ૞ᚑߒ㧘ߎߩᢔᏓ࿑

ណ᳓ᣇᴺ

߆ࠄ L ́ Q ߩ࿁Ꮻᑼࠍ᳞߼ߚ㧚ߎߩ L ́ Q ࿁Ꮻᑼߦ࿑  ߦ␜ߔฦᵹၞߩᵹᧃߦ޽ࠆ㊂᳓࠳ࡓ㧔᳓૏⸘

ᣣᲤߩᵹ㊂ࠍઍ౉ߒߡᣣᲤߩ THMFP ⽶⩄㊂ࠍ▚಴ߒ߇⸳⟎ߐࠇߡ޿ࠆ㧕ߩ5V  㧘5V  ෸߮ 5V  ߦߚ㧚ᣣᲤߩ⽶⩄㊂ࠍว⸘ߒߡ᦬ߩ⽶⩄㊂ߣߒ㧘ߐࠄߦ㧘߅޿ߡ㧘ේೣߣߒߡᲤ᦬ᧃߩ 㒠㔎ᤨࠍ㒰ߊᵹᴫ߇቟ቯ

᦬Ფߩ୯ࠍว⸘ߒߡᐕ㑆ߩ✚ᵹ಴⽶⩄㊂ߣߒߚ㧚ᐕ㑆ߒߚߣ߈ߦ ᷧᵹ᳓ࠍណ᳓ߒߚ㧚᦬ᧃߦណ᳓ߢ߈ߥ޿႐

✚⽶⩄㊂ࠍᵹၞ㕙Ⓧߢ㒰ߒߡᐕ㑆 ha ޽ߚࠅߩ୯ࠍ▚วߦߪ㧘⠉᦬ߩߢ߈ࠆߛߌᣧ޿ᤨᦼߦⴕߞߚ㧚㒠᳓ߦ಴ߒߚ㧚ߟ޿ߡߪ㧘࿑  ߦ␜ߔ᮸౰ߩᓇ㗀߇ߥ޿࿾ὐ R ߦ߅޿ߡ㧘࠺ࡐࠫ࠶࠻ᴺߦࠃࠅ  cm ࡠ࡯࠻ࠍ↪޿ߡ 㧟L ߩࡐ࡝࠲ࡦࠢߦ⾂⇐ߒߚ㧚

㧟࡮㧝

㧞࡮㧠

⺞ᩏ᳓⾰㗄⋡ߩ᷹ቯᣇᴺ㧞࡮㧠࡮㧝⚿ᨐ෸߮⠨ኤ
ᷧᵹ᳓ߩ᳓⾰ ᐕ  ᦬́ ᐕ  ᦬ߩ W ́㧘 W ́ ෸

6*/(2

߮ E ́ ߦ߅޿ߡ⺞ᩏߒߚ᳓⾰㗄⋡ߩᐔဋ୯෸߮ᮡ

THMFP ߩ᷹ቯᣇᴺߪ 㧘‫ ޟ‬THM ߦ㑐ߔࠆኻ╷ߦߟ޿ߡ‫ ✚ߩޠ‬THMFP ⹜㛎ᣇᴺ㧤㧕Ḱ஍Ꮕࠍ⴫  ߦ㧘߹ߚ㧘᦭ᯏ‛ߩᜰᮡߣߐࠇࠆ TOCߦḰߓߚ㧚ߔߥࠊߜ㧘⹜෸߮ E ߩផ⒖ࠍ࿑  ߦ␜ߔ㧚

᳓ߦㆡᒰ㊂ߩᰴ੝Ⴎ⚛㉄࠽࠻࡝࠙ࡓṁᶧࠍᷝടߒߡႮ⴫  ߆ࠄ᣿ࠄ߆ߥࠃ߁ߦ㧘 BOD ߩᐔဋ୯ߪ㧘ᴡᎹ⚛ಣℂ㧔R* 㧘 ͠㧘 ᤨ㑆㧕ߒ㧘ߘߩ⚿ᨐ㧘ᱷߩⅣႺၮḰ AA 㘃ဳ㧔 mg/L 㧕ࠍ޿ߕࠇ߽ਅ࿁ߞߡ߅

⇐Ⴎ⚛㊂߇ ́ OI. ߣߥࠆ߽ߩߦߟ޿ߡ㧘 THM ࠍࠅ㧘ߘߩ᳓⾰ߪ㕖Ᏹߦ⦟ᅢߢ޽ߞߚ㧚 ᰴߦ㧘᦭ᯏ‛ߩࡋ࠶࠼ࠬࡍ࡯ࠬ́)%/5ᴺߢ᷹ቯߒߚ㧚ᜰᮡߣߐࠇࠆ TOC ෸߮ ' ࠍߺࠆߣ㧘೨⠪ߦߟ޿ߡ㧞࡮㧠࡮㧞ߘߩઁߩ᳓⾰㗄⋡ߩ᷹ቯᣇᴺ

ߪᵹၞ㑆ߢᏅߪߺࠄࠇߥ߆ߞߚ㧚৻ᣇ㧘ᓟ⠪ߢߪᐔဋpH㧘㔚᳇વዉ₸㧔 EC㧕㧘ᶋㆆ‛⾰㧔 SS 㧕㧘↢‛ൻቇ́ ߢ޽ࠅ㧘ᵹၞ㑆ߢᏅ߇ߺࠄࠇߚ㧚ߐࠄ⊛ ㉄ ⚛ ᶖ ⾌㊂ 㧔 BOD㧕㧘ൻ ቇ ⊛ ㉄⚛ ᶖ ⾌ ㊂㧔 COD 㧕㧘ߦ㧘 TOC ෸߮ '  ߩផ⒖㧔࿑ 㧕ࠍߺࠆߣᄐቄߦ㜞ో᦭ᯏ὇⚛㧔 TOC㧕ߩ᷹ቯߦߟ޿ߡߪᎿ႐ឃ᳓⹜㛎ᴺ

ߊ㧘౻ቄߦૐߊߥࠆ௑ะߦ޽ߞߚ㧚⴫ฦ᳓⾰㗄⋡ߩᐔဋ୯㧘ᮡḰ஍Ꮕ㧘ᦨᄢ୯෸߮ᦨዊ୯

9

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ᵈ㧕R*㧘'%㧘55㧘61%㧘'㧘60߅ࠃ߮62ߪP㧘$1&߅ࠃ߮%1&ߪP㧘'ߪEOߩ࠮࡞ࠍ૶↪

 

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⽎ߣߒߡ߅ࠅ㧘⺞ᩏኻ⽎࿾ၞߩ㆑޿߇ᢙ୯ߦᄢ߈ߊ෻

㪫㪦㪚

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ᤋߒߡ޿ࠆ߽ߩߣផኤߐࠇࠆ㧚

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ᷧᵹ᳓ߩ6*/(2W ́㧘 W ́෸߮ E ́ߩ THMFP ࠍ⴫  ߦ␜ߔ 㧚

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ฦᵹၞߩ THMFP

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ᵈ㧕න૏㧦OI.

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䋱䋹䋹䋹

䋱䋹䋹䋸

࿑

㪉

W ́㧘 W ́ ෸߮ E ́ ߦ߅ߌࠆ THMFP ߩᐔဋ

TOC ෸߮ Eߩផ⒖୯ߪ㧘ߘࠇߙࠇ 㧘 ෸߮  mI . ߢ㧟࡮㧞޽ࠅ㧘නጀᨋߣⶄጀᨋߣߩ㆑޿ߦࠃࠆᏅߪߺࠄࠇߥ߆

6*/(2

㧟࡮㧞࡮㧝

ߞߚ㧚߹ߚ㧘 ᵹၞߩ THMFP ߪ ́ mI.㧘

㒠᳓ߩ6*/(2 ᐕ  ᦬ ́  ᐕ  ᦬ ߦ ߅ ߌ ࠆ 㒠 ᳓ ߩᐔဋ୯  mI . ߢ޽ߞߚ㧚ㆊ෰ߩ⺞ᩏ੐଀ߢߪ

THMFP ߩផ⒖ࠍ࿑  ߦ␜ߔ㧚㒠᳓ߩ THMFP ߪᴡ Ꮉᦨ ਄ᵹ ၞߩ ੱὑ ᵴ േߩᓇ 㗀 ߇ో ߊߥ ޿ᴡᎹ ᳓ߩ́ mg/L ߢផ⒖ߒߡ߅ࠅ㧘ᓟㅀߔࠆ㧔࡮

THMFP ߪ ́ mI.ߣႎ๔ߐࠇߡ޿ࠆ 㧝㧕㧚࡮ 㗄ߦ߅ߌࠆ㧕ᷧᵹ᳓ߩផ⒖㧔หᤨᦼ㧕ߣᲧセߔࠆႎ๔୯ߣ੹࿁ߩᢙ୯ࠍᲧߴࠆߣ㧘੹࿁ߩ⺞ᩏߢߪ৻ᩴ

ߣૐ୯ߢ޽ߞߚ㧚ߥ߅㧘ቄ▵ᄌേ╬ߦߟ޿ߡߪ  ᐕ㜞޿ᢙ୯߇ᓧࠄࠇߚ㧚㑆ߛߌߩ࠺࡯࠲ߩߚ߼᣿ࠄ߆ߢߥߊߎࠇߦߟ޿ߡߪ
੹ᓟߩ⺖㗴ߢ޽ࠆ㧚੹࿁ߩ⺞ᩏ⚿ᨐߪ  ᐕ㑆ߦࠊߚࠆ⛮⛯⺞ᩏߦၮߠ޿ߡᓧࠄࠇߚ߽ߢ޽ࠅ㧘ੱὑᵴേߩᓇ㗀߇߶ߣࠎߤߥ
޿ࠬࠡ࡮ࡅࡁࠠߩੱᎿᨋၞ߆ࠄߩ⥄ὼ↱᧪ߩ᦭ᯏ‛ߦ㪫㪟㪤㪝㪧䋨㫄㪾㪆㪣䋩㪇㪅㪇㪉㪇⿠࿃ߔࠆ THMFP ߩᐔဋ⊛ߥ࡟ࡌ࡞ࠍ⴫ߒߡ޿ࠆ߽ߩ
ߣផ᷹ߐࠇࠆ㧚߹ߚ㧘ߎߩ୯ߪ㧘ੱὑᵴേ╬ߦ↱᧪ߔ㪇㪅㪇㪈㪌ࠆ᦭ᯏ‛ߦ⿠࿃ߔࠆ THMFP ࠍ⹏ଔߔࠆ߁߃ߢߩࡃ࠶

㪇㪅㪇㪈㪇ࠢࠣ࡜࠙ࡦ࠼୯ߣߒߡ೑↪ߢ߈ࠆߣ⠨߃ࠄࠇࠆ 㧚ᰴߦ㧘 ᵹၞ೎ߦ߅ߌࠆ THMFP ߩផ⒖㧔࿑ 㧕ࠍߺ㪇㪅㪇㪇㪌ࠆߣ㧘ฦᵹၞߣ߽㧘 THMFP ߪᄐቄߦ㜞ߊ㧘౻ቄߦૐ

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࿑

㒠᳓ߩ THMFP ߩផ⒖ߊߥߞߡ߅ࠅ㧘ቄ▵ᄌേߩ޽ࠆߎߣ߇ಽ߆ߞߚ㧚

㪊᦬

㪇㪅㪇㪋㪇

㊀ߺઃߌᐔဋ୯ࠍ᳞߼ߚ㧚ߔߥࠊߜ㧘ߘߩᤨߩ㒠᳓㊂ߣỚᐲࠍਸ਼ߓߡ㒠᳓㊂ਛߦሽ࿷ߔࠆ‛⾰㊂ࠍ಴ߒ㧘ߘߩ✚๺ࠍ✚㒠᳓㊂ߢ㒰ߒߡ୯ࠍ▚಴ߒߚߣߎࠈ㧘 mg/L ߇ᓧࠄࠇߚ㧚ߎߩ୯ߪ㧘ᶆ㊁ࠄ 㧟㧕߇ႎ๔ߒߚ୯ 㧔 mg/L 㧕ߣᲧ セߔࠆߣਃಽߩ৻⒟ᐲߢ
޽ࠅ㧘߆ߥࠅૐ߆ߞߚ㧚ߎߩᢙ୯ߩ㆑޿ߦߟ޿ߡߪ㧘㪇㪅㪇㪊㪌㪫㪟㪤㪝㪧䋨㫄㪾㪆㪣䋩ᰴߦ㧘ߎࠇࠄߩ࠺࡯࠲ࠍ↪޿ߡ㒠᳓ߩ THMFP 㒠᳓

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ᷧᵹ᳓ߩ THMFP ߩផ⒖

ᶆ㊁ࠄߩ⺞ᩏ߇ᮮᵿᏒߣ޿߁ㇺᏒၞߩ㒠᳓ࠍኻ⽎ߣߒߡ޿ߚߩߦኻߒߡ㧘ᒰ⹥⺞ᩏߢߪ㧘ጊ㑆ㇱߩ㒠᳓ࠍኻ

 

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㪉 ᦬
䋲䋰䋰䋰

৻ᣇ㧘 ↢‛ߢߪಽ⸃ߐࠇߦߊߊߘߒߡਇ㘻๺⚿วࠍᓟ߇ᵹ಴ߔࠆߎߣ߇ಽ߆ߞߚ㧚ߥ߅㧘 ᐕߩ E ́

᦭ߔࠆ THMFP ߦ㑐ਈߔࠆ᦭ᯏ‛㧔↢‛㔍ಽ⸃ᕈ᦭ᯏ ߣ  ᐕ෸߮  ᐕߩ W ́ ߦߟ޿ߡߪ㧘᳓‛㧕ߩᜰᮡߣ ߥ ࠆ Eߩቄ▵ᄌേߪ㧘೨ㅀߒߚࠃ߁ ߦ૏⸘ߩਇౕวߢᵹ಴㊂ߦᰳ᷹╬߇↢ߓߚߚ߼㧘ᵹ಴₸

THMFP ߣห᭽ߦᄐቄߦ㜞ߊ౻ቄߦૐߊߥࠆ௑ะߦ޽

ߪ▚಴ߢ߈ߥ߆ߞߚ㧚ߞߚ㧔࿑ 㧕㧚 Eߩቄ▵ᄌേߦߟ޿ߡߪ㧘㊄ሶ 㧝㧜㧕߽ห㧟࡮㧠ᷧᵹ᳓ߩᵹ಴⽶⩄㊂᭽ߦᄐቄߦ㜞ߊ౻ቄߦૐߊߥࠆ௑ะߦ޽ࠆߎߣࠍᜰ៰

 ᐕ  ᦬́ ᐕ  ᦬ߩ THMFP ߣᣣᵹ㊂ߩߒ㧘ߘߩℂ↱ߣߒߡ㧘ᄐቄߦᷧᵹ᳓ਛߦ߅ߌࠆ☻ᄢ᦭࠺࡯࠲߆ࠄ ࡮ 㗄ߦ␜ߔᣇᴺߦࠃࠅ㧘 W ́ ෸߮ Eᯏ‛㧔᫪ᨋᵹၞ߆ࠄᵹ಴ߔࠆ߽ߩߢ⪭⪲߿⪭ᨑ߇ᄢඨ́ ߦ߅ߌࠆᣣᵹ㊂㧔 Q㧕ߣ THMFP ⽶⩄㊂ L ߣߩ㑐ࠍභ߼ࠆ㧕ߩಽ⸃߇ㅴⴕߔࠆߚ߼ߣ⠨߃ࠄࠇࠆߣႎ๔

ଥࠍ␜ߔ࿁Ꮻᑼ㧔 L = aQ 㧕ࠍ᳞߼ߚ㧚ߘߩ⚿ᨐࠍ⴫ߒߡ޿ࠆ㧚 ߦ␜ߔ㧚bᰴߦ㧘 THMFP ߣߘߩઁߩ㗄⋡㧔᳓᷷㧘 E㧘 TOC㧕⴫ߣߩ㑐ଥࠍᬌ⸛ߒߚߣߎࠈ㧘⴫  ߦ␜ߔ⚿ᨐ߇ᓧࠄࠇ㧘 THMFP ߣߘߩઁߩ㗄⋡ߣߩ 㑆ߦߪ޿ߕࠇ߽ 㧝%ߩෂ㒾₸ߢ᦭ᗧߥ⋧㑐߇޽ࠆߎߣ߇ಽ߆ߞߚ㧚

ᵹၞฬ

P

9
'

ߎࠇࠄߩߎߣ߆ࠄ㧘 ᵹၞߦሽ࿷ߒߡ޿ࠆ᦭ᯏ‛ߩಽ⸃߇ᄐቄߦ⋓ࠎߦⴕࠊࠇ㧘ߘߩ⚿ᨐ㧘ᄐቄߦߪ
THMFP ߦ㑐ਈߔࠆ↢‛㔍ಽ⸃ᕈ᦭ᯏ‛߇౻ቄߦᲧߴ

‫ޓ‬6*/(2

'

᳓᷷

‫ޓ‬᳓᷷



㧖

‫'ޓ‬‫ޓ‬61%



㧖



㧖



㧖

9

㧖

'









ߩෂ㒾₸ߢ᦭ᗧߦ⋧㑐ߩ޽ࠆߎ

THMFP ᵹ಴⽶⩄㊂ߩᄌൻࠍ࿑  ߦ␜ߔ㧚





㪫㪟㪤㪝㪧⽶⩄㊂䋨㪾㪆㪿㪸䋩㪈㪌㪇㒠᳓㊂㧘ᵹ಴㊂෸߮ᵹ಴₸








ߩᣣᵹ㊂ࠍઍ౉ߒߡ᦬೎ߩ⽶⩄㊂ࠍ▚಴ߒߚ㧚᦬೎ߩᵹ಴㊂෸߮ᵹ಴₸ࠍ⴫  ߦ␜ߔ㧚ᐕ

ߎߩ L ́ Q ᑼߦ  ᐕ  ᦬߆ࠄ  ᐕ  ᦬W ́ ෸߮ E ́ ߦ߅ߌࠆ́ ᐕߩ㒠᳓㊂㧘

ᵹၞฬ



ߣ߇ಽ߆ߞߚ㧚㒠᳓㊂㧘ᵹ಴㊂෸߮ᵹ಴₸⴫

Tቯߩ⚿ᨐ߆ࠄ㧘 %61%‫ޓ‬ᵈ㧕P㧔ᐕ᦬ᐕ᦬ߩ࠺࡯࠲㧕‫ޓޓޓ‬㧖ߪߢ᦭ᗧ㧟࡮㧟

DW ́ ෸߮ E ́ ߦ߅ߌࠆ L ߣ Q ߣߩ㑆ߦߪ t ᬌ


㧖

C

ᵈ㧕P㧦࠺࡯࠲ᢙ㧘.㧦Iᣣ㧘3㧦O ᣣ㧘T㧦⋧㑐ଥᢙ

THMFP ߣઁߩ㗄⋡ߣߩ㑐ଥ
6*/(2.C3D㧟

ߡᄙ㊂ߦᷧᵹ᳓ਛߦሽ࿷ߒߡ޿ࠆߣផኤߐࠇࠆ㧚⴫

THMFP ߩ࿁Ꮻᑼߩቯᢙߣ⋧㑐ଥᢙ

㒠᳓㊂
OO㧕





ᵹ಴㊂
OO㧕


̆


㪮㪄㪈
㪜㪄㪋
㪈㪇㪇

㪌㪇

㪇
㪈

ᵹ಴₸
㧔㧕


̆


㪊

㪌

㪎

㪐

㪈㪐㪐㪐

࿑

㪈㪈

㪈

㪊

㪌

㪎

㪐

㪈㪈 ᦬

㪉㪇㪇㪇

᦬೎ߩ THMFP ᵹ಴⽶⩄㊂ߩᄌൻ࿑  ߆ ࠄ᣿ࠄ߆ߥࠃ߁ߦ㧘ᷧᵹ᳓ߩ᦬೎ THMFPᵹ಴⽶⩄㊂ߪ㧘 ᐕ෸߮  ᐕߣ߽  ᦬߇ᦨᄢߣߥߞߚ㧚ᒰ⹥ᵹၞߩᐔဋ㒠᳓㊂ߪ㧘⚂  mm ߢ޽ࠆ 㧚ᰴߦ㧘᦬೎ߩ⽶⩄㊂ࠍว⸘ߒߡᐕ㑆ߩ✚ᵹ಴⽶⩄㊂

ᐔဋ㒠᳓㊂ߣᲧセߒߡ㧘 ᐕߩ㒠᳓㊂ߪ㧘 W ́ߣߒ㧘ᐕ㑆ߩ✚⽶⩄㊂ࠍᵹၞ㕙Ⓧߢ㒰ߒߡᐕ㑆 ha ޽ߢᄙ㔎௑ะߦ޽ࠅ㧘߹ߚ㧘 ᐕߩ㒠᳓㊂ߪ㧘ਔᵹߚࠅߩᵹ಴⽶⩄㊂ࠍ⹜▚ߒߚ㧚ᐕ㑆 ha ޽ߚࠅߩᵹ಴

ၞߣ߽ዋ㔎௑ะߦ޽ߞߚ㧚ᰴߦ㧘 W ́ ෸߮ E ́

⽶⩄㊂ࠍ⴫  ߦ␜ߔ㧚ߩᵹ಴₸ࠍ᳞߼ߚ㧚⴫  ߆ࠄ᣿ࠄ߆ߥࠃ߁ߦਔᵹၞ

ߥ߅㧘 ᐕߩ E ́ ߣ  ᐕ෸߮  ᐕߩߩߘࠇߪ́ %W ́ ߦߟ޿ߡߪ㧘᳓૏⸘ߩਇౕวߢᵹ಴㊂ߦᰳ᷹㧕ߦ޽ࠅ㧘㒠᳓㊂ߩ⚂  ഀ೨

 

ᢥ₂

╬߇↢ߓߚߚ߼㧘ᵹ಴⽶⩄㊂ߪ▚಴ߢ߈ߥ߆ߞߚ㧚⴫  ߆ࠄ᣿ࠄ߆ߥࠃ߁ߦ㧘 THMFP ߩᐕ㑆ᵹ಴㊂ߪ㧘㧔Σ㧕́ ́ ́㧘㧚 ᐕߩ W ́ ߢ  g/ha㧘߹ߚ㧘 ᐕߩ W ́ ෸߮ E ́ ߢߘࠇߙࠇ  ෸߮  g/ha ߢ޽ࠅ 㧘






6*/(2㧔IJC࡮ᐕ㧕
ᐕ

ᐕ

9





'

̆



⋧ᴛ⾆ሶࠄ㧦᳓⾰ᳪỘ⎇ⓥ㧘㧔㧕㧘-㧘㧚

THMFP ߩᵹ಴⽶⩄㊂㧔 g/ha࡮ᐕ㧕ᵹၞฬᶆ㊁⚔ᐔࠄ㧦᳓㆏දળ㔀⹹㧘㧔㧕㧘-㧘㧚ⷐߢ޽ࠆߣ⠨߃ࠄࠇࠆ㧚⴫᳗ᷗ⟵ቁࠄ㧦↪᳓ߣᑄ᳓㧘㧔㧕㧘-㧘㧚߶߷ㄭૃߒߚᢙ୯߇ᓧࠄࠇߚ㧚ߒ߆ߒ㧘ߎࠇࠄߩᢙ୯
ߩ♖ᐲࠍ㜞߼ࠆߦߪ㧘ᦝߥࠆ࠺࡯࠲ߩ෼㓸࡮⫾Ⓧ߇ᔅว↰ஜࠄ㧦ᤘ๺  ᐕᐲⅣႺ଻ో⎇ⓥᚑᨐ㓸⑔ፉඳࠄ㧦᳓⾰ᳪỘ⎇ⓥ㧘㧔㧕㧘-㧘
㧚



Ⓑ᫪ᖘᐔࠄ㧦᳓ⅣႺቇળ⹹㧘㧔 㧕㧘 - 㧘
㧚



૒‫ᧁޘ‬㊀ⴕࠄ㧦⑔ጟ⋵ᨋᬺ⹜㛎႐ᤨႎ㧘╙ภ㧘
㧚



ෘ↢⋭ⅣႺⴡ↢ዪ㧦᳓㆏ⅣႺㇱ㐳ㅢ⍮㧔Ⅳ᳓╙ภ 㧕㧘㧚

㧠



߹ߣ߼

ᣣᧄⷙᩰදળ㧦 JIS K  Ꮏ႐ឃ᳓⹜㛎ᣇᴺ㧘᧲੩㧘㧚ࠬࠡ࡮ࡅࡁࠠߩੱᎿᨋࠍਥߣߔࠆ᫪ᨋၞ߆ࠄᵹ಴ߔ
ࠆᷧᵹ᳓ࠍኻ⽎ߦ⚂  ᐕ㑆ߦࠊߚߞߡ THMFP ╬ߦ ㊄ሶ⌀ม㧦ḓᴧߢߩ᦭ᯏ‛ߩേᘒ⸃ᨆᚻᴺߩ㐿⊒ଥࠆ᳓⾰⺞ᩏࠍⴕߞߚߣߎࠈ㧘એਅߩߎߣ߇᣿ࠄ߆ߣ

ߦ㑐ߔࠆ⎇ⓥ㧘ㄘᨋ᳓↥⋭ㄘᨋ᳓↥ᛛⴚળ੐ോዪ 㧘

ߥߞߚ㧚

㧚
 ┻ਅᢘมࠄ㧦⑔ጟ⋵ᨋᬺ⹜㛎႐ᤨႎ㧘╙ภ㧘㧕 W ́㧘 W ́ ෸߮ E ́ ߦ߅ߌࠆᷧᵹ᳓ߩ㧚

THMFP ᐔဋ୯ߪ㧘ߘࠇߙࠇ㧘 ෸߮ m I. ߢ޽ࠅ㧘⺞ᩏᵹၞ㑆ߢߩᏅߪߺࠄࠇߥ
߆ߞߚ㧚㧕ᷧᵹ᳓ߩ THMFP ߪ㧘ᦨᄢߢ  mI. ࠍ␜ߒߚ㧚㧕⺞ᩏᵹၞߦ߅ߌࠆ㒠᳓ߩ THMFP ᐔဋ୯㧔㒠᳓㊀ߺઃߌ㧕ߪ㧘 mg/L ߢ޽ߞߚ㧚㧕ᷧᵹ᳓ߩ THMFP ߪᄐቄߦ㜞ߊ㧘౻ቄߦૐߊߥߞ
ߡ߅ࠅ㧘ቄ▵ᄌേ߇ߺࠄࠇߚ㧚㧕ᷧᵹ᳓ߩ࠻࡝ࡂࡠࡔ࠲ࡦ↢ᚑ⢻ߣਇ㘻๺⚿วࠍ᦭ߔࠆ᦭ᯏ‛ߩᜰᮡߣߥࠆ⚡ᄖ✢ๆశᐲ㧔 E 㧕ߣߩ㑆ߦߪᱜߩ⋧㑐߇޽ߞߚ㧚㧕ᷧᵹ᳓ߩ᦬೎ THMFP ᵹ಴⽶⩄㊂ߪ㧘 ᐕ෸߮ ᐕߣ߽  ᦬߇ᦨᄢߣߥߞߚ㧚㧕⺞ᩏᐕ㧔 ᐕ෸߮  ᐕ㧕ߢߩ W ́ ෸߮߮ E ́ ߦ߅ߌࠆ THMFP ߩᐕ㑆ᵹ಴⽶⩄㊂ߪ㧘́  g/ha ߢ޽ߞߚ㧚 Trihalomethane formation potentials in stream waters from forested mountains
Yoshitaka NAGAFUCHI 㧖, Hiroshi MATSUO 㧖 and Shigeyuki SASAKI 㧖㧖 Fukuoka Institute Health and Environmental Sciences
Mukaizano  Dazaifu Fukuoka ́ Japan㧖 Fukuoka Forest Research and Technology Center
Yamamoto ́ machi ́ Kurume Fukuoka ́ JapanAn investigation on water quality was conducted using stream waters from three basins (W ́ 1, W ́ 4 and E ́ 4) on aforested mountain. Water quality and runoff loads of trihalomethane formation potential (THMFP) were estimated. THMFP in Ẃ 1, W ́ 4 and E ́ 4 were 0.019, 0.022 and 0.023 mg/L, respectively. It was observed that THMFP in stream waters from 3
basins varied with the seasons. There was a positive correlation between THMFP and ultraviolet absorbance at 260 nm (E260) as
an indicator of organic matter containing an unsaturated bond. The annual runoff loads of THMFP were estimated in the range of
220 ́ 340 g/ha in two basins (W ́ 1 and E ́ 4).
㨇Key words : Stream waters, Trihalomethane formation potential, Runoff loads?

 ⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ╙ภ㧘㧙㧘ේ⪺⺰ᢥ
4&(὾ළἯࠍ೑↪ߒߚ࿾⋚᧚ᢱߩⅣႺᓇ㗀㠽⠀ፄ᮸㧘࿯↰ᄢテ㧘㜞ᯅᶈม㧘㤥Ꮉ㓁৻㧘᳗ἑ⺈㧘ቝㇺችᓃ࿾⋚᧚ᢱߢ޽ࠆࡐ࠱࠹࠶ࠢ4ߪ㧘4&(὾ළἯ㧘⍹὇Ἧ෸߮ឃᾍ⣕⎫ࠬ࡜࠶ࠫࠍේᢱߣߒ㧘࿕ൻ߇චಽ
ߦㅴⴕߒߚࡩ᦬ᓟߦߪ㧘ᒝᐲၮḰ෸߮㊀㊄ዻ㘃ߩ࿯ფⅣႺၮḰࠍḩ⿷ߒ㧘㆏〝╬ߩ࿾⋚᧚ᢱߣߒߡߩ
ᕈ⢻ࠍ᦭ߔࠆߎߣ߇⏕⹺ߐࠇߡ޿ࠆ㧚ᧄ⎇ⓥߢߪ㧘ᧄ᧚ᢱ߇࿕ൻߦࠃߞߡ㊀㊄ዻ㘃ߩṁ಴ࠍᛥ೙ߔࠆ
ߎߣࠍ⏕⹺ߔࠆߚ߼㧘R*ߩ▸࿐ߢߩ㊀㊄ዻ㘃ߩṁ಴㊂ࠍ⺞ᩏߒ㧘ߘࠇࠄߩR*▸࿐ߢߪ߶ߣࠎߤߩ
႐ว㧘4&(὾ළἯߣᲧセߒߡૐߊᛥ߃ࠄࠇߡ޿ࠆߎߣࠍ⏕⹺ߒߚ㧚߹ߚ㧘⵾ㅧᓟೋᦼߦ㋦߇ṁ಴ߒߚߎߣ߆ࠄ㧘㋦ṁ಴㊂ࠍ⚻ᣣ⊛ߦㅊ〔ߒߚ⚿ᨐ㧘⵾ㅧᓟᓢ‫ߦޘ‬ṁ಴㊂߇ᷫዋߒㅳ㑆⒟ᐲߢ࿯ფⅣႺၮḰ୯ࠍḩ⿷ߔࠆߎߣ߇ಽ߆ߞߚ㧚ߎߩߣ߈ߩ:✢࿁᛬ಽᨆ⚿ᨐ߆ࠄ㧘ࠞ࡞ࠪ࠙ࡓࠕ࡞ࡒࡀ࡯࠻߇㧘㋦ṁ಴ᛥ೙ߦ㑐ਈߒߡ޿ࠆߎߣ߇᣿ࠄ߆ߣߥߞߚ㧚ᦝߦ㧘⵾ㅧᓟೋᦼߩ㋦ṁ಴ࠍᛥ೙ߔࠆᣇᴺࠍᬌ⸛ߒߚ⚿ᨐ㧘⎫㉄ࠕ࡞ࡒ࠾࠙ࡓࠍᷝടߒ㧘ṁ಴⹜㛎ᶧߩR*ࠍએਅߣߥࠆࠃ߁ߦ▤ℂߔࠇ߫㧘಴⩄ᤨ ⵾ㅧ㧝ᣣᓟ
ߩ㋦ṁ಴㊂ࠍ࿯ფⅣႺၮḰ୯એਅߦᛥ೙ߢ߈ߚ㧚㨇ࠠ࡯ࡢ࡯࠼ 4&(὾ළἯ㧘᦭ല೑↪㧘ࡐ࠱࠹࠶ࠢ4㧘቟ోᕈ⹏ଔ㧘㋦㨉㧝᷹ቯ෸߮࿯ფⅣႺၮḰߦḰߓߚ㊀㊄ዻ㘃ߩṁ಴⹜㛎ߦࠃߪߓ߼ߦ⃻࿷ߩᑄ᫈‛ಣℂߦ߅޿ߡߪᑄ᫈‛὾ළᤨߩ࠳ࠗࠝࠠ

ࠆⅣႺ቟ోᕈ⹏ଔࠍⴕߞߚ⚿ᨐ㧘ᒝᐲ෸߮ⅣႺ቟ోᕈߦ

ࠪࡦ㘃ߩኻ╷߿ᦨ⚳ಣಽ႐ߩਇ⿷ߣ޿ߞߚ⺖㗴ߦขࠅ⚵߅޿ߡ㆏〝╬ߩ࿾⋚᧚ᢱߣߒߡߩᕈ⢻ࠍ᦭ߔࠆߎߣ߇⏕ࠎߢ޿ࠆߣߎࠈߢ޽ࠆ㧚ᐔᚑᐕ᦬ߩ࠳ࠗࠝࠠࠪࡦ㘃⹺ߐࠇ㧘ᐔᚑᐕ᦬ߦ⑔ጟ⋵ᑪ⸳ᛛⴚᖱႎ࠮ࡦ࠲࡯ߩ

ⷙ೙ᒝൻ߳ߩኻᔕߣߒߡ㧘㜞᷷ߢ቟ቯ⊛ߥㅪ⛯Ά὾߇น᳃㑆㐿⊒ᛛⴚክᩏ⸽᣿ࠍขᓧߒߚ 㧚⢻ߥᣉ⸳ᢛ஻߇ᔅⷐߢ޽ߞߚ㧚ߒ߆ߒ㧘ዊⷙᮨᏒ↸᧛නࡐ࠱࠹࠶ࠢ4ߪᣉᎿᓟᓢ‫ߦޘ‬࿕ൻ߇ㅴߺ㧘ᒝᐲ߇⊒⃻⁛ߢߪ㧘ߎߩᣉ⸳ᢛ஻߇㔍ߒ޿ߩ߇⃻⁁ߢ޽ߞߚߎߣ߆

ߔࠆߣߣ߽ߦ㊀㊄ዻ㘃ߩṁ಴߽ᛥ೙ߐࠇࠆߣ⠨߃ࠄࠇߡࠄ㧘⑔ጟ⋵ߢߪ㧘ዊⷙᮨᏒ↸᧛ߩߏߺ὾ළߦ઻߁࠳ࠗࠝ

޿ࠆ㧚ߎߩߚ߼㧘ㅢᏱߩࠦࡦࠢ࡝࡯࠻߇቟ቯൻߔࠆߣ⠨ࠠࠪࡦ㘃ኻ╷෸߮ߏߺߩᾲ࿁෼ࠍ࿑ࠆߚ߼㧘ᐔᚑᐕ

߃ࠄࠇߡ޿ࠆࡩ᦬ᓟߩṁ಴⹜㛎ߦࠃࠆⅣႺᓇ㗀⹏ଔߢ㧘᦬㧘ᄢ—↰Ꮢ㧘㔚Ḯ㐿⊒ࢃߣߣ߽ߦ㧘ᄢ—↰࡝ࠨࠗࠢ࡞

㊀㊄ዻ㘃ߩṁ಴߇࿯ფⅣႺၮḰ୯એਅߣߥࠆߎߣ߇⏕⹺⊒㔚ᩣᑼળ␠ࠍ⸳┙ߒ㧘4&(⊒㔚੐ᬺࠍផㅴߒߡ޿ࠆ㧚ߐࠇߡ޿ߚ㧚ᧄ⺰ᢥߢߪ㧘ࡐ࠱࠹࠶ࠢ4ߩⅣႺᓇ㗀ߦ㑐ᦝߦ㧘 4&(⊒㔚ᚲߩⒿ௛ߦ઻ߞߡឃ಴ߐࠇࠆ὾ළἯߦߟ

ߔࠆᬌ⸛ߩߥ߆ߢ㧘㊀㊄ዻ㘃ߩṁ಴ߦߟ޿ߡߩR*ଐሽᕈ޿ߡߪ㧘ၒ┙ಣಽ㊂ߩᷫኈൻ෸߮⾗Ḯ▵⚂ߩߚ߼ߦ㧘᦭⹜㛎  㧘ೋᦼߩ㋦ṁ಴ߦߟ޿ߡߩ⚻ᣣᄌൻ෸߮ṁ಴ᛥ೙ല೑↪ᣇᴺߣߒߡ㧘὾ළἯࠍ〝⋚᧚╬ߦ೑↪ߔࠆࡐ࠱࠹

ᣇᴺߩᬌ⸛ ߦߟ޿ߡ⸥ㅀߒߚ㧚࠶ࠢ4ߩታ↪ൻ⹜㛎ࠍⴕߞߚ㧚㧞ࡐ࠱࠹࠶ࠢ4ߪ㧘⍹὇Ἧ㧘4&(὾ළἯ㧘ឃᾍ⣕⎫ࠬ࡜࠶

⹜㛎ᣇᴺ

㧞࡮㧝

R*ଐሽᕈ⹜㛎ࠫ㧔੝⎫㉄ࠞ࡞ࠪ࠙ࡓ㧕෸߮࠮ࡔࡦ࠻ߦ㧘ㆡ㊂ߩ᳓ࠍടࡐ࠱࠹࠶ࠢ4ߣߘߩේᢱߩ߁ߜ4&(὾ළἯ㧔ንጊ⋵ධ⎘߃ߡ☳⁁૕ߣߒߡ㧘⃻႐ߢォ࿶ᣉᎿߔࠆ߽ߩߢ޽ࠅ㧘᧚࡝ࠨࠗࠢ࡞࠮ࡦ࠲࡯↥಴ߩ4&(ࠍታ⸽⹜㛎⸳஻ߢΆ὾ߒ

ᢱߩਥᚑಽߢ޽ࠆࠞ࡞ࠪ࠙ࡓ㧘ࠕ࡞ࡒ࠾࠙ࡓ෸߮ࠤࠗ⚛

ߡᓧࠄࠇߚ߽ߩ㧕෸߮⍹὇Ἧ㧔਻Ꮊ㔚ജ᷼⊒㔚ᚲ߆ࠄឃ╬߇᳓๺෻ᔕߦࠃߞߡ࿕ൻߔࠆߎߣࠍ೑↪ߒߚ߽ߩߢ޽

಴ߐࠇߚ߽ߩ㧕ߦߟ޿ߡ㧘R*ଐሽᕈ⹜㛎ࠍⴕߞߚ㧚ߎࠇࠆ㧚߹ߚ㧘ᧄ⎇ⓥߩߥ߆ߢේᢱߩ㈩วഀวߩᬌ⸛㧘ᒝᐲࠄ⹜㛎ኻ⽎‛ߩ㊀㊄ዻ㘃฽᦭㊂ࠍ⴫ߦ␜ߔ㧚⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲ㧔‫ ޥ‬ᄥቿᐭᏒᄢሼะ૒㊁㧕 ߚ㧚ߎࠇࠄߩ⹜ᢱ Iߦ⫳⇐᳓ ONࠍട߃㧘⎣㉄෸߮⴫㪈䇭䊘䉹䊁䉾䉪㪩෸䈶䈠䈱ේᢱ䈱㊀㊄ዻ㘃฽᦭㊂
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㩿㫄㪾㪆㫂㪾㪀 㩿㫄㪾㪆㫂㪾㪀 㩿㫄㪾㪆㫂㪾㪀 㩿㫄㪾㪆㫂㪾㪀 㩿㫄㪾㪆㫂㪾㪀 㩿㫄㪾㪆㫂㪾㪀᳓㉄ൻ࠽࠻࡝࠙ࡓṁᶧࠍ↪޿ߡ㧘R*ࠦࡦ࠻ࡠ࡯࡜࡯ߢR*㧘㧘㧘㧘㧘෸߮ߦ⺞ᢛߒ㧘ࡑࠣࡀ࠴࠶ࠢࠬ࠲

䊘䉹䊁䉾䉪㪩

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㪉㪅㪐

㪈㪉㪇

㪈㪊㪇

㪇㪅㪎

㪊㪅㪌

䌒䌄䌆὾ළἯ

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㪈㪈㩷㩷

㪍㪇㪇

㪋㪋㪇

㪈㪅㪎

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ࡉ࡜ࡦࡈࠖ࡞࠲࡯ߢࠈㆊߒߡ㊀㊄ዻ㘃ಽᨆ⹜ᢱߣߒߚ㧚

⍹὇Ἧ

㪇㪅㪈㪏

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߹ߚ 㧘ⅣႺᐡ๔␜╙ภߦࠃࠆṁ಴⹜㛎ࠍหᤨߦⴕߞߚ㧚࡯࡜࡯ߢᤨ㑆ᠣᜈߒߚ㧚ߘߩᓟ㧘ሹᓘ ǴOߩࡔࡦ4&(὾ළἯ෸߮⍹὇Ἧߦߟ޿ߡߪ᦭ᆫߩ߹߹૶↪ߒ㧘㧞࡮㧞㋦ṁ಴㊂ߩ⚻ᣣᄌൻ෸߮㋦ṁ಴ᛥ೙ᣇᴺߩᬌ⸛

ࡐ࠱࠹࠶ࠢ4ߦߟ޿ߡߪ㧘ᒝᐲ⹜㛎ߩߚ߼ߦ૞ᚑߐࠇࠆ

⵾ㅧᓟೋᦼߩ࿕ൻ߇චಽߦㅴࠎߢ޿ߥ޿⁁ᘒߢߪ㧘㋦⋥ᓘ EO㧘㜞ߐ EO⒟ᐲߩ౞ᩇ⁁ߩଏ⹜૕ࠍᒝᐲ⹜㛎ߩṁ಴߇⷗ࠄࠇߚߎߣ߆ࠄ㧘ṁ಴௑ะࠍ᣿ࠄ߆ߦߔࠆߚ

ᓟߦ⎕⎈ߒ㧘߰ࠆ޿ߦ߆ߌߡ⋥ᓘ OOએਅߣߒߡ૶↪ߒ߼⚻ᣣᄌൻࠍ⺞ߴߚ㧚⹜ᢱߦߪ㧘ታ㓙ߩ⵾ຠߣหߓ㈩ว

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⍹὇Ἧ

䌒䌄䌆὾ළἯ

⍹὇Ἧ䊘䉹䊁䉾䉪㪩

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䌒䌄䌆὾ළἯ

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⍹὇Ἧ

䌒䌄䌆὾ළἯ

䊘䉹䊁䉾䉪㪩

䋨䂦䋬䂾䋬䂔䈲䋬ⅣႺᐡ๔␜╙㪋㪍ภ䈮䉋䉎ṁ಴⹜㛎⚿ᨐ䋩࿑㪊䇭㫇㪟ଐሽᕈ⹜㛎㩿㪚㫉㩿㸌㪀㪀࿑㪋䇭㫇㪟ଐሽᕈ⹜㛎㩿㪚㪻㪀

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㪈㪉㫇㪟䋨䂦䋬䂾䋬䂔䈲䋬ⅣႺᐡ๔␜╙㪋㪍ภ䈮䉋䉎ṁ಴⹜㛎⚿ᨐ䋩

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䌒䌄䌆὾ළἯ

࿑㪉䇭㫇㪟ଐሽᕈ⹜㛎㩿㪪㪼㪀࿑䋱䇭㫇㪟ଐሽᕈ⹜㛎㩿㪧㪹㪀㪉㪍䋨䂦䋬䂾䋬䂔䈲䋬ⅣႺᐡ๔␜╙㪋㪍ภ䈮䉋䉎ṁ಴⹜㛎⚿ᨐ䋩䋨䂦䋬䂾䋬䂔䈲䋬ⅣႺᐡ๔␜╙㪋㪍ภ䈮䉋䉎ṁ಴⹜㛎⚿ᨐ䋩

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⍹὇Ἧ

䌒䌄䌆὾ළἯ

⍹὇Ἧ䊘䉹䊁䉾䉪㪩㪍㪏㫇㪟䌒䌄䌆὾ළἯ㪈㪇㪈㪉㪈㪋䊘䉹䊁䉾䉪㪩

䋨䂦䋬䂾䋬䂔䈲䋬ⅣႺᐡ๔␜╙㪋㪍ภ䈮䉋䉎ṁ಴⹜㛎⚿ᨐ䋩䋨䂦䋬䂾䋬䂔䈲䋬ⅣႺᐡ๔␜╙㪋㪍ภ䈮䉋䉎ṁ಴⹜㛎⚿ᨐ䋩࿑㪍䇭㫇㪟ଐሽᕈ⹜㛎㩿㪘㫊㪀࿑㪌䇭㫇㪟ଐሽᕈ⹜㛎⚿ᨐ㩿㪟㪾㪀 ഀวߢ૞ᚑߒߚଏ⹜૕ࠍḰ஻ߒ㧘⹜㛎ᣣ߹ߢ ͠ߩᕡNߢ޽ߞߚ㧚ߒ߆ߒ㧘ࡐ࠱࠹࠶ࠢ4ߪ㧘᳓๺࿕ൻߦࠃࠆല

᷷ቶߦ⟎޿ߚ㙃↢▫㧔ੇ῎ࠍ㒐ߋߚ߼ 㧘ਅߦ᳓ࠍߪߞߡ㧘ᨐ߇⷗ࠄࠇ㧘R*એਅ෸߮R*એ਄ߢߪ OIN㧘R*ߘߩ਄ㇱߦ✂ࠍᒛࠅଏ⹜૕ࠍ଻ሽߔࠆ߽ߩ㧕ਛߦ଻ሽߒ

ߢ OIN㧘R*ߢ OINߢ޽ߞߚ㧚ࠞ࠼ࡒ࠙ࡓߦ

ߚ㧚ߎࠇࠍ㧘⹜㛎ᣣߏߣߦขࠅ಴ߒߡ☳⎈ߒ㧘 OOߩࡔߟ޿ߡߪ㧘ో⹜ᢱߦ߅޿ߡR*એ਄ߢߪ OINߢ޽࠶ࠪࡘࠍㅢㆊߒߚ߽ߩࠍⅣႺᐡ๔␜╙ภߦߒߚ߇ߞߡ

ߞߚ߇㧘4&(὾ළἯߢߪ㧘R*ߩ▸࿐ߢṁ಴߇⷗ࠄࠇ㧘ṁ಴⹜㛎ࠍታᣉߒߚ㧚߹ߚ㧘ߎߩߣ߈ਗⴕߒߡ:✢࿁᛬․ߦR*ߢߪ OINߩ㜞Ớᐲߩṁ಴߇⷗ࠄࠇ
ߚ㧚ࡐ࠱࠹࠶ࠢ4ߦߟ޿ߡߪR*એ਄ߢߪ OINએਅಽᨆࠍⴕ޿㧘㋦ṁ಴ᛥ೙ࡔࠞ࠾࠭ࡓߩ⸃᣿ࠍ⹜ߺߚ㧚߹ߚ㧘㋦ߩṁ಴ᛥ೙ᣇᴺߩᬌ⸛ߣߒߡ㧘ԘR*ࠍૐਅߐߢ޽ߞߚ㧚᳓㌁ߦߟ޿ߡߪ㧘R*એਅ෸߮R*ߢ㜞Ớᐲߖߡ㋦ߩṁ಴ࠍᛥ೙ߐߖࠆߚ߼㧘࠮ࡔࡦ࠻ᷝട㊂ࠍㅢᏱߩṁ಴௑ะ߇⷗ࠄࠇߚ㧚ࡐ࠱࠹࠶ࠢ4ߦߟ޿ߡߪ㧘R*એߩ ߦኻߒߡ㧘 㧘 ߦᷫዋߐߖࠆታ㛎㧘ԙࠫ࠴ࠝ਄ߢߪ OINߢ޽ߞߚ㧚ࡅ⚛ߪ㧘⍹὇Ἧ෸߮4&(ࠞ࡞ࡃࡒࡦ㉄♽ߩࠠ࡟࡯࠻೷ࠍ4&(὾ළἯߦኻߒߡ 㧘 㧘὾ළἯߩR*ߣR*ߢᲧセ⊛㜞޿ṁ಴௑ะ߇޽ߞߚ㧚㋦ 㧘 ෸߮ ᷝടߔࠆታ㛎㧘Ԛ⎫㉄ࠕ࡞ࡒ࠾࠙ࡓ╬એᄖߩ㊀㊄ዻ㘃ߦߟ޿ߡ߽㧘߶ߣࠎߤߩ႐ว࿕ൻߔࠆߎ

ࠍᷝടߒߡ㧘R*ࠍ⒟ᐲߦૐਅߐߖ㧘㋦ߩṁ಴ࠍᛥߣߦࠃߞߡṁ಴ᛥ೙ലᨐ߇ߺࠄࠇߚ㧚೙ߔࠆታ㛎ࠍታᣉߒߚ㧚ᷝട‛ߣߒߡ⎫㉄ࠕ࡞ࡒ࠾࠙ࡓ

㧟࡮㧞㋦ṁ಴㊂ߩ⚻ᣣᄌൻ╬ࠍㆬࠎߛℂ↱ߪ㧘ᚑಽߣߒߡࡐ࠱࠹࠶ࠢ4ߦ߽ߣ߽ߣ

ࡐ࠱࠹࠶ࠢ4߆ࠄߩ㋦ߩṁ಴㊂ߪ㧘࿑ߦ␜ߔࠃ߁ߦ㧘฽᦭ߐࠇ㧘R*એᄖߩᓇ㗀߇ዋߥ޿ߣ⠨߃ࠄࠇߚߚ߼ߢ޽

ᣣ⋡ߦ OINߢ޽ߞߚ߇㧘㗅ᰴᥳᷫߒߡᣣ⋡ߦ࿯ࠆ 㧚 ԙ෸߮Ԛߩታ㛎ߢߪ 㧘⹜ᢱߪ㧘ଏ⹜૕ࠍ૞ᚑߖߕ㧘

ფⅣႺၮḰ  OIN ࠍਅ࿁ߞߚ 㧚߹ߚ㧘ᣣ⋡ߦߪ 㧘⚗⁁૕ߩ߹߹ቶ᷷ߢ᡼⟎ߒߚ㧚ᬌ಴㒢⇇୯ߩ OINᧂḩߣߥߞߚ㧚Ԛߩታ㛎ߢߪ㧘ᮡḰ㈩วߩࡐ࠱࠹࠶ࠢ4 ⍹὇Ἧ㧘4&(㪇㪅㪈㪇㪇὾ළἯ෸߮ឃᾍ⣕⎫ࠬ࡜࠶ࠫߩഀว㧔㊀㊂Ყ㧕߇
ߢ 㧘࠮ࡔࡦ࠻ࠍᄖഀߢᷝടߒߚ߽ߩ ࠍኻᾖߣߒߡ 㧘⎫㉄ࠕ࡞ࡒ࠾࠙ࡓࠍᷝടߒߚ߽ߩ㧘߹ߚ 㧘ߎࠇߦട߃ߡ㧘㪇㪅㪇㪏㪇ᒝᐲࠍჇߔߚ߼ߦ࠮ࡔࡦ࠻ᷝട㊂ࠍჇടߒߡߣߒߚ߽
ߩ㧘4&(὾ළἯߩഀวࠍჇ߿ߒߚ߽ߩ㧘ᦝߦ㧘⎫㉄ߩߺᷝടߒߚ߽ߩߦߟ޿ߡ㧘ᣣᓟߦṁ಴⹜㛎ࠍⴕߞߚ㧚⚿ᨐ෸߮⠨ኤ

㧟࡮㧝㪧㪹㩿㫄㪾㪆㫃㪀

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R*ଐሽᕈ⹜㛎⹜㛎⚿ᨐࠍ࿑ߦ␜ߒ㧘ห৻⹜ᢱߢታᣉߒߚⅣႺᐡ๔␜╙ภߦࠃࠆṁ಴⹜㛎⚿ᨐࠍ⊕ᛮ߈ߩࡑ࡯ࠞ࡯ߢ૬

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⸥ߒߚ㧚㋦ߪ㧘࿑ߦ␜ߔࠃ߁ߦ㧘ోߡߩ⹜ᢱߦ߅޿ߡ
R*ߢߪṁ಴㊂ߪ OINߢ޽ࠅ㧘㗼⪺ߥṁ಴ߪ⷗ࠄࠇߥ߆ߞߚ㧚ߒ߆ߒ㧘਄⸥એᄖߩR*▸࿐ߢߪ㧘㪇㪅㪇㪉㪇

4&(὾ළἯ߆ࠄ㋦߇ṁ಴ߒ㧘ߘߩṁ಴㊂ߪ㧘 R*㧘෸߮ߢߘࠇߙࠇ㧘 OIN㧘 OIN෸߮ OINߢ޽ߞߚ㧚ࡐ࠱࠹࠶ࠢ4ߩᮡḰ⊛㈩ว㊂ߢߪ㧘4&(὾ළἯߪ㊀㊂㪇㪅㪇㪇㪇Ყߢో૕ߩ⚂ ߣߥࠆߚ߼㧘㈩วഀว߆ࠄ⸘▚ߔࠆߣ 㧘R*㧘෸߮ߢߘࠇߙࠇ OIN㧘 OIN෸߮

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࿑㪎䇭㋦ṁ಴㊂⚻ᣣᄌൻ OINߣߥࠆߪߕߢ޽ࠆ߇㧘ታ㓙ߪ㧘ߘࠇߙࠇ OI
N㧘 OIN෸߮ OINߢ޽ࠅ㧘⸘▚୯ࠍ߆ߥࠅ

䉶䊜䊮䊃䋳䋦

䉶䊜䊮䊃䋱䋦

䉶䊜䊮䊃䋰䋦

䉨䊧䊷䊃೷䋱䋦

䉨䊧䊷䊃೷䋲䋦

䉨䊧䊷䊃೷䋵䋦

䉨䊧䊷䊃೷䋰䋦

ਅ࿁ߞߡ޿ࠆ㧚ߎߩߎߣߪ㧘ࡐ࠱࠹࠶ࠢ4߇᳓๺෻ᔕߦ
߹ߚ㧘㋦ߩṁ಴ᛥ೙ᯏ᭴ࠍ⸃᣿ߔࠆߚ߼㧘ࡐ࠱࠹࠶ࠢ

ࠃߞߡ࿕ൻߒ㧘ߎߩߣ߈㧘㋦ࠍ᳓๺࿕ൻ‛ߩਛߦኽߓㄟ
߼ࠆߎߣߦࠃߞߡṁ಴ࠍᛥ೙ߒߡ޿ࠆߣ⠨߃ࠄࠇࠆ㧚4ߩਥᚑಽߢ޽ࠆࠞ࡞ࠪ࠙ࡓ㧘ࠕ࡞ࡒ࠾࠙ࡓ෸߮ࠤࠗ⚛

࠮࡟ࡦߪ㧘⍹὇Ἧਛߩ฽᦭㊂߇㜞޿ߚ߼㧘⍹὇Ἧ߆ࠄࠍ฽߻ൻว‛ߩ⚿᥏᭴ㅧߩᄌൻࠍ㨄✢࿁᛬ߢಽᨆߒߚ㧚ߩṁ಴㊂ߪ㧘R*ߩ▸࿐ߢ㜞ߊ㧘 OINߢ޽ਥߥࡇ࡯ࠢࠍ࡜ࠗࡉ࡜࡝߆ࠄᬌ⚝ߒߚ⚿ᨐ㧘%C#N1%N㨯ߞߚ㧚߹ߚ㧘4&(὾ළἯߪ㧘R*ߩ▸࿐ߢᲧセ⊛㜞޿*1㧔ࡈ࡝࡯࠺࡞᳁Ⴎ 㧕㧘%C#N 51  1* 㨯*1㧔ࠛ࠻ṁ಴௑ะ߇⷗ࠄࠇߚ㧚౐ଔࠢࡠࡓߣߒߡߩṁ಴㊂ߪ㧘R*࡝ࡦࠟࠗ࠻ 㧕㧘%C51㨯*1㧔੝⎫㉄ࠞ࡞ࠪ࠙ࡓ㧕㧘#N5K

ߩ▸࿐ߢᲧセ⊛㜞ߊ㧘․ߦ4&(὾ළἯߢ OI

1㧔ࡓ࡜ࠗ࠻㧕෸߮5K1㧔ࠢࠝ࡯࠷㧕߇ሽ࿷ߒߡ޿ࠆߣ ⠨߃ࠄࠇߚ㧚࿑ࠃࠅ㧘ǰqߩࠛ࠻࡝ࡦࠟࠗ࠻ߣᕁ

ൻߒߥ߆ߞߚ㧚߹ߚ㧘ࡓ࡜ࠗ࠻෸߮ࠢࠝ࡯࠷ߪ㧘ర᧪቟ࠊࠇࠆࡇ࡯ࠢߣǰqߩࡈ࡝࡯࠺࡞᳁Ⴎߣᕁࠊࠇࠆ

ቯߥൻว‛ߢ޽ࠅ㧘᳓๺෻ᔕߦࠃߞߡ⚿᥏᭴ㅧ߇ᄌൻߒ

ࡇ࡯ࠢ߇ᣣࠍㅊߞߡ㧘ᚑ㐳ߒߡ޿ࠆߎߣ߇᣿ࠄ߆ߢ޽ࠅ 㧘ߥ޿ߣ⠨߃ࠄࠇࠆ㧚ǰqߩࠢࠝ࡯࠷ߣᕁࠊࠇࠆࡇ

㋦ߩṁ಴ᛥ೙ᯏ᭴ߦߎࠇࠄߩൻว‛ߩ㑐ਈ߇␜ໂߐࠇ

࡯ࠢ߇ᄌേߒߡ޿ࠆ߇㧘ⷙೣᕈߪߥߊ㧘⹜ᢱߩ߫ࠄߟ߈

ࠆ㧚߹ߚ㧘੝⎫㉄ࠞ࡞ࠪ࠙ࡓߪ㧘ឃᾍ⣕⎫ࠬ࡜࠶ࠫߦ↱ߢ޽ࠆߣ⠨߃ࠄࠇࠆ㧚᧪ߔࠆ߽ߩߢ޽ࠆߚ߼㧘᳓๺෻ᔕߦࠃߞߡ⚿᥏᭴ㅧ߇ᄌ

ࡈ࡝࡯࠺࡞᳁Ⴎ

㧟࡮㧟ࠛ࠻࡝ࡦࠟࠗ࠻

੝⎫㉄ࠞ࡞ࠪ࠙ࡓ

㋦ṁ಴ᛥ೙ᣇᴺߩᬌ⸛

ࡓ࡜ࠗ࠻

ࠢࠝ࡯࠷

࡟࡯࠻೷ࠍᔅⷐߣߒ㧘ᣣᓟߩᛥ೙ലᨐߪ㧘ࠠ࡟࡯࠻೷

࿑ߦ␜ߒߚ⚻ᣣᄌൻߩ⚿ᨐࠃࠅ㧘⵾ㅧᓟㅳ㑆ߪ㋦߇

ࠍᷝടߒߥ޿႐วߣหߓߢ޽ࠆߎߣ߇ಽ߆ߞߚ㧚ᰴߦ㧘⎫㉄ࠕ࡞ࡒ࠾࠙ࡓߩᷝടߦࠃࠆ㋦ṁ಴ᛥ೙ߩᬌ

߮⎫㉄ࠕ࡞ࡒ࠾࠙ࡓߩᷝടߦࠃࠆṁ಴ᛥ೙ߦߟ޿ߡߩታ

⸛ࠍⴕߞߚ㧚ߘߩ⚿ᨐ㧘࿑ߦ␜ߔࠃ߁ߦ㧘ṁ಴⹜㛎ᶧ㛎ࠍⴕߞߚ㧚࿑ߦ␜ߔߣ߅ࠅೋᦼߩ㋦ṁ಴ᛥ೙ߦ㑐ߒ

ߩR*߇એਅߩ႐ว㧘࿯ფⅣႺၮḰ୯  OIN ࠍߡ 㧘࠮ࡔࡦ࠻ᷝട㊂ߩᏅߦࠃࠆ㆑޿ߪ߶ߣࠎߤߥ߆ߞߚ 㧚ḩ⿷ߔࠆߎߣ߇ಽ߆ߞߚ㧚ߎࠇࠄߩታ㛎߆ࠄ㧘ᮡḰ㈩ว

߹ߚ㧘ࠠ࡟࡯࠻೷ߩᷝടߦࠃࠆ㋦ߩṁ಴ᛥ೙ߪ㧘⵾ㅧᓟߩࡐ࠱࠹࠶ࠢ4ߦ⎫㉄ࠕ࡞ࡒ࠾࠙ࡓࠍㆡ㊂ᷝടߔࠆߎߣ

ೋᦼߦࠠ࡟࡯࠻೷ᷝട㊂߇ᄙ޿߶ߤ㋦ߩṁ಴߇ᛥ೙ߐࠇ

ߢ㧘⵾ㅧᣣᓟߦ㋦ߩṁ಴ࠍ᛼ߐ߃ࠆߎߣ߇น⢻ߢ㧘ᒝࠆ௑ะ߇⷗ࠄࠇߚ߇㧘ᣣ⋡ߢߪࠠ࡟࡯࠻೷ࠍᷝടߒߥ

ᐲ⊛ߦ߽ၮḰࠍḩߚߔߎߣ߇ߢ߈ߚ㧚⎫㉄ࠕ࡞ࡒ࠾࠙ࡓ

߆ߞߚ߽ߩߣᄢ߈ߥᏅߪߥ߆ߞߚ㧚ߎߩߎߣࠃࠅ㧘ࠠ࡟

ߩᷝട㊂ߪ㧘ዋߥߔ߉ࠆߣR*߇ਅ߇ࠄߕ㧘ᄙߔ߉ߡR*߇

࡯࠻೷ߪහലᕈ߇޽ࠅ㧘ೋᦼߦߪ㋦ߩṁ಴ࠍᛥ߃ࠆലᨐ

ਅ߇ࠅߔ߉ࠆߣᒝᐲ߇⪭ߜࠆߚ߼㧘R*߇ߩ▸߇㜞޿߇㧘࿯ფⅣႺၮḰࠍḩ⿷ߔࠆߚ߼ߦߪ㧘ᄢ㊂ߩࠠ࿐ߦߥࠆࠃ߁ߦ೨⹜㛎ߦࠃࠅ⏕⹺ߒߡ᳿ቯߒߚ㧚ᮡḰ㈩

㪇㪅㪈㪇

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㪇㪅㪇㪍

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วߩࡐ࠱࠹࠶ࠢ4ߩ႐ว㧘 ߇ㆡᒰߢ޽ߞߚ㧚߹ߚ㧘4&(὾ළἯߩഀวࠍჇ߿ߒߡ߽㧘⎫㉄ࠕ࡞ࡒ࠾࠙ࡓߩᷝട
㊂ࠍჇ߿ߖ߫㧘ၮḰࠍḩߚߔߎߣ߇น⢻ߢ޽ߞߚ㧚㫇㪟

㋦ṁ಴㊂㩿㫄㪾㪆㫃㪀ṁ಴ߔࠆߎߣ߇ಽ߆ߞߚߚ߼㧘࠮ࡔࡦ࠻㧘ࠠ࡟࡯࠻೷෸

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R*ଐሽᕈ⹜㛎ߢߪ㧘ේᢱߩ4&(὾ළἯߪ㧘R*ߦࠃߞߡߪ㋦╬㊀㊄ዻ㘃ߩ㜞Ớᐲߩṁ಴߇⷗ࠄࠇߚ߇㧘ࡐ࠱࠹࠶ࠢ4߆ࠄߩ㋦ߩṁ಴㊂ߪR*ߩ▸࿐ߦ߅޿ߡૐ߆ߞߚ㧚ߎߩߎߣ߆ࠄ㧘᳓๺࿕ൻߦࠃߞߡ㊀㊄ዻ㘃߇㧘࿕ൻ‛ਛߦขࠅㄟ߹ࠇ㧘ṁ಴ߒߦߊߊߥࠆ੐߇ಽ߆ߞߚ㧚߹ߚ㧘ࡐ࠱࠹࠶ࠢ4ࠍ⚻ᣣ⊛ߦ:✢࿁᛬ಽᨆߒߚ⚿ᨐ㧘ࠛ࠻࡝ࡦࠟࠗ࠻߿ࡈ࡝࡯࠺࡞᳁Ⴎ╬ߩࠞ࡞ࠪ࠙ࡓࠕ࡞ࡒࡀ࡯࠻
߇㧘㋦ṁ಴ᛥ೙ߦ㑐ਈߒߡ޿ࠆߎߣ߇ಽ߆ߞߚ㧚࿑䋹䇭䌰䌈ૐᷫ⹜㛎⚿ᨐ㪧㪹㩿㫄㪾㪆㫃㪀

㧠߹ߣ߼㫇㪟㋦ṁ಴㊂ߩ⚻ᣣᄌൻ߆ࠄ㧘ࡐ࠱࠹࠶ࠢ4ߪ㧘⵾ㅧᓟ৻ㅳ㑆⒟ᐲߪ㋦ṁ಴㊂߇࿯ფⅣႺၮḰ୯ࠍ⿥߃ࠆߎߣ߇ಽ

 

ᢥ₂

߆ߞߚ㧚
ߎߩߚ߼㧘ೋᦼߩ㋦ṁ಴ࠍᛥ೙ߔࠆᣇᴺࠍᬌ⸛ߒߚ⚿

⽷࿅ᴺੱ⑔ጟ⋵ᑪ⸳ᛛⴚᖱႎ࠮ࡦ࠲࡯㧦4&(ߩᵹേᐥᨐ㧘⎫㉄ࠕ࡞ࡒ࠾࠙ࡓࠍᷝടߒ㧘ṁ಴⹜㛎ᶧߩR*ࠍ

Ά὾ߦࠃࠆἯࠍᷙ౉ߒߚ࿾⋚᧚ᢱ‫ࠢ࠶࠹࠱ࡐޟ‬4‫ޠ‬㧘એਅߣߥࠆࠃ߁ߦ▤ℂߔࠇ߫㧘಴⩄ᤨߩ㋦ṁ಴㊂ࠍ࿯ფ᳃㑆㐿⊒ᛛⴚ࡮ክᩏ⸽᣿੐ᬺߦၮߠߊ᳃㑆㐿⊒ᛛⴚⅣႺၮḰ୯એਅߦᛥ೙ߢ߈ࠆߎߣ߇ಽ߆ߞߚ㧚ክᩏ⸽᣿ႎ๔ᦠ㧘
㠽⠀ፄ᮸ࠄ㧦4&(὾ළἯߣߘߩ᳓๺࿕ൻ‛߆ࠄߩ㊀㊄⻢ㄉዻ㘃ṁ಴․ᕈ㧘╙࿁ᑄ᫈‛ቇળ⎇ⓥ⊒⴫ળ⻠Ṷ⺰ᧄ⎇ⓥߪ㧘࡝ࠨࠗࠢ࡞✚ว⎇ⓥ࠮ࡦ࠲࡯ߩࡊࡠࠫࠚࠢ

ᢥ㓸㧘-㧘

࠻⎇ⓥߦ߅޿ߡ㧘ਃ੗㋶ጊࢃ㧘㔚Ḯ㐿⊒ࢃ㧘਻Ꮊᄢቇ෸

㠽⠀ፄ᮸ࠄ㧦4&(὾ළἯ╬ߩ᳓๺෻ᔕߦࠃࠆ㋦ṁ಴ᛥ߮଻ஜⅣႺ⎇ⓥᚲ߇౒หߢታᣉߒߚ߽ߩߢ޽ࠅ㧘ࡐ࠱࠹೙ᯏ᭴ߦߟ޿ߡ㧘╙࿁ᑄ᫈‛ቇળ⎇ⓥ⊒⴫ળ⻠Ṷ

࠶ࠢ4ߩ⵾ㅧ෸߮ᒝᐲ⹜㛎╬ߦ㑐ߔࠆߎߣߪਃ੗㋶ጊࢃ⺰ᢥ㓸㧘-㧘

ߢⴕߞߡ޿ࠆ㧚ߎߎߦ㧘㑐ଥฦ૏ߦᗵ⻢ߩᗧࠍ⴫ߔࠆ㧚㧔⧷ᢥⷐᣦ㧕

Environmental Impact of Road Bed Material Containing Fly Ash Generated from
Refuse-Derived Fuel Incinerator
Mineki TOBA, Daisuke TSUCHIDA, Koji TAKAHASHI, Youichi KUROKAWA, Makoto NAGASE andAkira UTSUNOMIYA
Fukuoka Institute of Health and Environmental Sciences
Mukaizano  Dazaifu Fukuoka - Japan
Road bed material “Poz-O-Tec R”, which contains fly ash generated from Refuse-Derived Fuel (RDF) incinerator, ismanufactured to promote the recycling of RDF fly ash. The properties of the material were investigated by the pH-dependentleaching test conducted in the range of pH2-13 using the batch type leaching test (Japanese Leaching Test No.46; JLT46) toevaluate the environmental impact on groundwater when this material is used as road bed. The result of pH-dependentleaching test shows that the concentrations of leached heavy metals from the road bed material are relatively smaller thanthose leached from its components, such as coal fly ash and RDF fly ash. And the amounts of heavy metals released from thematerial during JLT46 meet the environmental standards for all heavy metals except lead. It is confirmed that theconcentration of lead by JLT46 decreases with consolidation of the road bed material and meets the environmental standards
within 7 days after construction using this material. X-ray analysis indicates that calcium aluminate inhibit the leaching oflead. It is found that the initial high concentration of lead within 7 days can be controlled by adding aluminum sulfate which
lowers the pH of the material to 12.3 or less.

=Key words; RDF incinerated ash, Effective utilization, Poz-O-Tec R, Safety evaluation, Lead?

  

⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ╙ภ㧘㧙㧘⾗ᢱᐔᚑᐕᐲߩ㘩ਛᲥ㧔⇼޿ࠍ฽߻㧕੐଀ߦߟ޿ߡ
ၳᎹ๺⟤㧘㊁↰ᄙ⟤ᨑ㧘ự㦮శብ㧘᧛਄శ৻㧘┻ਛ㊀ᐘ㧘⍹㤤㕏ዏ㧘਎⦟ᥰਯ㧘⍹ᯅື਽㧘ᳯ⮮⦟᮸㧘ජ‫ޘ‬๺ൎᏆ⑔ጟ⋵ߦ߅޿ߡᐔᚑᐕᐲߦ⊒↢ߒߚ㘩ਛᲥ㧔⇼޿ࠍ฽߻㧕੐଀㧔ᬌ૕㧕ߦߟ޿ߡ㧘ਥߣߒߡ∛࿃‛⾰ߩⷰὐ߆ࠄ੐଀ࠍ߹ߣ߼ߚ㧚ᧄᐕᐲߪ㧘ᤐቄ߆ࠄ⑺ቄߦ߅޿ߡߪ⣺Ἳࡆࡉ࡝ࠝࠍߪߓ߼ߣߔ
ࠆ⚦⩶ᕈ㘩ਛᲥ߇㧘౻ቄߦߪࡁࡠ࠙ࠗ࡞ࠬࠍේ࿃ߣߔࠆ࠙ࠗ࡞ࠬᕈ㘩ਛᲥ߇ᄙߊ⷗ࠄࠇߚ㧚∛ේᓸ↢‛߇ᬌ಴ߐࠇߚ੐଀ߪ੐଀ਛ੐଀㧔%㧕ߢ޽ߞߚ㧚∛ේᓸ↢‛೎ߦ⷗ࠆߣ㧘⣺Ἳࡆࡉ࡝ࠝߦࠃ
ࠆ߽ߩ߇੐଀㧔%㧕㧘ࠨ࡞ࡕࡀ࡜ߦࠃࠆ߽ߩ߇੐଀㧔%㧕㧘࠮࡟࠙ࠬ⩶ߦࠃࠆ߽ߩ߇੐଀㧔%㧕
෸߮ࡁࡠ࠙ࠗ࡞ࠬߦࠃࠆ߽ߩ߇੐଀㧔%㧕ߢ޽ߞߚ㧚ߘߩઁߦ⣺▤಴ⴊᕈᄢ⣺⩶㧻㧘ࡆࡉ࡝ࠝ࡮ࡒࡒࠞࠬ㧘㤛⦡ࡉ࠼࠙⃿⩶෸߮ࡁࡠ࠙ࠗ࡞ࠬߣਅ∯ේᕈᄢ⣺⩶ߣߩᷙวᗵᨴߥߤߦࠃࠆ㘩ਛᲥ㧔⇼޿ࠍ฽߻㧕੐଀߇޽ߞߚ㧚ߒ߆ߒ㧘∛࿃‛⾰߇್᣿ߒߥ޿੐଀߇੐଀㧔%㧕޽ࠅ㧘੹ᓟߩᬌ⸛⺖㗴߽ᄙ޿㧚৻ᣇ㧘ᐕᐲᒰೋߦ⊒↢ߒߚࡁࡠ࠙ࠗ࡞ࠬߣਅ∯ේᕈᄢ⣺⩶ߣߩᷙวᗵᨴ੐଀ߪ㧘ᒰ⋵ౝߩห৻ᣉ⸳ߢኋᴱߥ޿ߒ㘩੐޽ࠆ޿ߪ౉ᶎࠍߒߚߎߣࠍ౒ㅢߣߒ㧘ᖚ⠪߇਻Ꮊ⋵ߦ߹ߚ߇ࠆᄢⷙᮨ㘩ਛᲥߢ޽ߞߚ㧚
㨇ࠠ࡯ࡢ࡯࠼ 㘩ਛᲥ㧘⣺Ἳࡆࡉ࡝ࠝ㧘ࠨ࡞ࡕࡀ࡜㧘ࡁࡠ࠙ࠗ࡞ࠬ㧘ᷙวᗵᨴ㨉㧝ߪߓ߼ߦ㘩ຠᱷຠ㧘᜞߈ขࠅ㧘⩶ᩣߥߤ㧕ߦߟ޿ߡ㧘㘩ਛᲥ⚦⩶∛ේᓸ↢‛ߦࠃࠆᗵᨴ∝ߪ㧘ක≮߿౏ⴐⴡ↢ߩะ਄ߦ߽߆߆ࠊࠄߕ 㧘ᷫዋ௑ะߪ⹺߼ࠄࠇߥ޿ 㧚ߘߩਛߢ߽㧘㘩ຠࠍ੺ߒߡ⊒↢ߔࠆ㘩ਛᲥߪ㧘㘩ᢥൻߩᕆỗߥᄌൻߦࠃࠅ㧘ᄙ᭽ൻߒᐢၞ߆ߟᄢⷙᮨൻߒߡ޿ࠆ㧚⣺▤಴ⴊᕈᄢ⣺⩶1ߦࠃࠆ㘩ਛᲥߪ㧘ᐔᚑᐕࠍᄾᯏߦ㓸࿅੐଀߇ᄙ⊒ߒ㧘㓸࿅෸߮ᢔ⊒੐଀ߣ߽ߦჇടߩ௑ะߦ޽ࠆ㧚߹ߚ㧘⣺Ἳࡆࡉ࡝ࠝߩ㘩ਛᲥߢߪ㧘⿒∯⩶ߥߤߣห᭽ャ౉㘩ຠߦ⿠࿃ߔࠆ߽ߩ߽ᄙ޿㧚৻ᣇߢ㧘㘩ਛᲥේ࿃⩶ߣ
ߪᢿቯߒ㔍޿࠮࡟࠙ࠬ⩶ߩࠃ߁ߥ࿯ფ↱᧪⩶߿ⅣႺߦᐢ
ߊಽᏓߔࠆ㤛⦡ࡉ࠼࠙⃿⩶ߥߤߩ㘩ਛᲥ߽ᓟࠍ⛘ߚߥ෸߮࠙ࠗ࡞ࠬߦߟ޿ߡᬌᩏࠍታᣉߒߚ㧚ᖚ⠪ߩ∝⁁ߥߤ߆ࠄ⚦⩶ᕈ㘩ਛᲥ߇⇼ࠊࠇࠆ႐วߪ㧘߹ߕ៝౉ߐࠇߚᬌ૕ࠍ㧘ࠕ࡞ࠞ࡝ࡍࡊ࠻ࡦ᳓㧘 %㘩
Ⴎടࡉ࡛ࠗࡦ㧘ࡊ࡟ࠬ࠻ࡦ㧘࡜ࡄࡐ࡯࠻࡮ࡃࠪ࡝ࠕ࠺ࠖ
ࠬၭ࿾ߥߤߩၭ࿾ࠍ↪޿ߡჇ⩶ၭ㙃ߒ 㧘TCBS ኙᄤၭ࿾㧘㘩Ⴎෆኙᄤၭ࿾㧘ࠬࠠࡠ࡯ኙᄤၭ࿾㧘 SM-ID ኙ ᄤၭ࿾ߥߤߢಽ㔌ၭ㙃ߒߚ㧚ኙᄤᐔ᧼ၭ࿾ߦ⇼ࠊߒ޿㓸⪭߇⊒⢒ߒߚ႐วߪ㧘㊒⩶ߒߡ㧘 TSI㧘 SIM ߥߤࠍ↪޿ߚ↢ൻቇᕈ⁁⹜㛎㧘ⴊᷡဳ೎㧘Ქ⚛ဳ೎㧘 Polymerase

chain

reaction (2%4ᴺ ࠍ↪޿ߚ∛ේㆮવሶߩᬌ಴ߥߤߩ⹜㛎ᬌᩏࠍታᣉߒߡ㘩ਛᲥ⚦⩶ߩหቯࠍⴕߥߞߚ㧚޿㧚ߎࠇࠄߩේ࿃ߣߒߡߪ㧘ᱜⷙᓥᬺຬ߇ዋߥߊ㧘⛮⛯৻ᣇ 㧘࠙ࠗ࡞ࠬᕈ㘩ਛᲥߣ⠨߃ࠄࠇࠆ႐วߪ㧘♮ଢ㧔ᢙ⊛ߥⴡ↢⽿છ߇ᰳᅤߒߡ޿ࠆߥߤ␠ળ⊛໧㗴߇⢛᥊ߦ޽

ࠣ࡜ࡓ⒟ᐲ㧕ࠍ࡝ࡦ㉄✭ⴣᶧߢ㧑੃೷ߣߒ㧘 TRO

ࠆߎߣุ߽߼ߥ޿㧚৻ᣇ㧘ో࿖࡟ࡌ࡞ߩߺߥࠄߕ࿾ၞߦ

ߢಽ㑆㆙ᔃᓟ 㧘ߘߩ਄ᷡࠍTROߢಽ㑆㆙ᔃߒߚ㧚߅ߌࠆ㘩ਛᲥ੍㒐ࠍ⠨߃ࠆߣ߈㧘⑔ጟ⋵ߢ⊒↢ߒ⹦⚦ߦߎߩ਄ᷡ߆ࠄ40#ࠍ᛽಴ߒ㧘ㅒォ౮㉂⚛ࠍ↪޿ߡ⋧⵬⊛ᬌᩏߒߚ੐଀ߏߣߦᬌ⸽ߔࠆߎߣ߽㊀ⷐߢ޽ࠆ㧚੹࿁㧘

ߥ&0#ࠍวᚑߒ㧘ࡁࡠ࠙ࠗ࡞ࠬߩㆮવሶߦ․⇣⊛ߥࡊ࡜ᐔᚑᐕᐲߦ⋵ၞߢ⊒↢ߒߚ㘩ਛᲥ㧔⇼޿ࠍ฽߻㧕੐଀ࠗࡑ࡯ࠍ↪޿ߡ2%4ߢჇ᏷ߒ㧘Ⴧ᏷↥‛ࠍ㔚᳇ᵒേߢ⏕ߦߟ޿ߡ㧘∛࿃‛⾰ߩ․ᓽߥߤࠍ⸃ᨆߒߚ㧚ߘߩ⚿ᨐߦ⹺ߒߚ㧚Ⴧ᏷↥‛߇⏕⹺ߐࠇߚᬌ૕ߦߟ޿ߡߪߐࠄߦࠪ

ߟ޿ߡႎ๔ߔࠆ㧚࡯ࠢࠛࡦࠬࠍⴕߞߡߘߩჇ᏷↥‛ߩႮၮ㈩೉ࠍ᳿ቯߒ㧘㘩ਛᲥ⊒↢ᤨߩᬌᩏ

ࡁࡠ࠙ࠗ࡞ࠬߩᦨ⚳⏕⹺෸߮ㆮવሶဳߩ᳿ቯࠍⴕߞߚ㧚ᐔᚑᐕᐲߪ 㧘੐଀㧘ᬌ૕㧔ᖚ⠪ଢ 㧘ᓥ੐⠪ଢ㧘߹ߚ㧘৻ㇱߦߟ޿ߡߪ㧘਄⸥਄ᷡࠍ⿥㆙ᔃಽ㔌㧔

⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲ

㧔‫ޥ‬ᄥቿᐭᏒᄢሼะ૒㊁㧕

 

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ᐔᚑᐕᐲߩ㘩ਛᲥ㧔⇼޿ࠍ฽߻㧕੐଀ߩ᭎ⷐ

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ේ࿃㘩ຠ

੐ઙߩ᭎ⷐ

ਇ᣿
㐳ፒ⋵߆ࠄ╳ᓟᣇ㕙ߦᣏⴕਇ᣿⠧ੱ⑔␩ᣉ⸳ߢߩ⊒↢ਇ᣿╳ᓟᏒౝߩᣉ⸳ߦ㑐ࠊࠆ⺞ᩏࠬࡄࠥ࠹ࠗ ࠠࡁࠦࠍ૶ߞߚࠬࡄࠥ࠹ࠗࠍ㘩ߒߡਇ᣿╳ᓟᏒౝߩᣉ⸳ߦ㑐ࠊࠆ⺞ᩏ♯ደ㇭ౝߢ⠧ੱࠍ᜗ᓙߒ㧘ᑯᒰࠍឭଏ
ਇ᣿㧔ߔߒ㧕 ࠬ࡯ࡄ࡯ߢ⾼౉ߒߚኼมࠍ⾼౉ߒኅᐸߢ៨㘩⋵ౝߩᅚᕈ߇૒⾐⋵ౝߢኋᴱߒߡ
ਇ᣿
ᑯᒰ
ਇ᣿
ਇ᣿

⑔ጟᏒߢ㐿௅ߐࠇߚࠗࡌࡦ࠻ߢឭଏߐࠇߚᑯᒰࠍ㘩ߒߡ⋵ౝߩኅᣖ߇ਅ㑐Ꮢࠍ⸰ࠇ㧘⚿⚊ᓟߩ㘩੐߇ឭଏߐࠇߚ

╳⚡㊁Ꮢౝߩ὾⡺ᐫߢ὾߈⡺╬ࠍ៨㘩ߒߡ⥄ኅ⵾ࡑ࡛ࡀ࡯࠭ ౏౒࿅૕ᣉ⸳ߢ⽼ᄁߐࠇߡ޿ࠆࠨࡦ࠼ࠗ࠶࠴ࠍ㘩ߒߡቬ௝Ꮢౝൕോߩ⡯႐ห௥߇ᑯᒰࠍ㘩ߒߡਇ᣿㧔઀಴ߒ㧕 ਛᵤᏒౝߢ⵾ㅧߐࠇߚ઀಴ߒࠍೋ⋆ળߩᓟߦ㘩ߒߡ㧔઀಴ߒ㧕 ↰ᎹᏒౝߢ⵾ㅧߐࠇߚ઀಴ߒࠍೋ⋆ળߩᓟߦ㘩ߒߡߚ޿ࠄ߉ߩ⽴ᩇ ਛᵤᏒߢ઀౉ࠇߚャ౉⽴ᩇࠍ㘩ߒߡ

ਇ᣿
ࠁߢࠛࡆ

ࡈࠔࡒ࡝࡯࡟ࠬ࠻࡜ࡦߢቯ㘩ࠍ៨㘩ߒߡ

᳃ኋߦኋᴱߒ㧘ೝりߥߤࠍ៨㘩ߒߡࠪࡘ࡯ࠢ࡝࡯ࡓ ᄢಽ⋵ౝߩࡅ࠻߇ਭ⇐☨Ꮢౝߢ⵾ㅧߐࠇߚࠪࡘ࡯ࠢ࡝࡯ࡓࠍ㘩ߒߡ㧔઀಴ߒ㧕 ཅⓄ㇭ౝߩኅᣖ߇㘵ႦᏒౝߩ㘶㘩ᐫߢળ㘩
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ኅᐸౝ੐଀
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ኅᐸౝ੐଀
♯ደ㇭ౝߣർ਻ᎺᏒౝߩࡅ࠻߇⑔ጟᏒౝߢળ㘩
ਇ᣿
ਇ᣿
ኅᐸౝ੐଀
ฎ⾐Ꮢ࿅૕ߩ⡯ຬ߇⑔ᵤᏒౝߢળ㘩ਇ᣿⋵ౝߩኅᣖฬ߇ᄢಽ⋵ౝߢኋᴱߒߡ
ਇ᣿ർ਻ᎺᏒౝߩኅᣖ߇ਛ㑆Ꮢౝߩ㘶㘩ᐫߢળ㘩ਇ᣿ⴕᯅᏒౝߦൕോߩ⡯႐ห௥߇ਭጊ↸ߢળ㘩ਇ᣿㕷ᚻ㇭ౝߩኅᣖ߇㘵ႦᏒౝߩ⚿ᇕᑼ႐ߢળ㘩ਇ᣿૒⾐⋵ߩࠣ࡞࡯ࡊ߇ਭ⇐☨Ꮢౝߩ㘶㘩ᐫߢળ㘩ਇ᣿ਇ᣿ኅᣖ߇὾⡺ᐫߢ὾߈⡺╬ࠍ៨㘩ਇ᣿ਭ⇐☨Ꮢౝߩ⡯႐࿅૕߇ᣂᐕળ
ਇ᣿㜞ᩞ↢ୃቇᣏⴕฎ⾐Ꮢౝߩ⡯႐࿅૕߇㘶㘩ᐫߢળ㘩ਇ᣿ਭ⇐☨Ꮢ㧘૒⾐⋵ౝ࿷૑ߩ⡯႐ห௥߇ਭ⇐☨Ꮢౝળ㘩ਇ᣿ኅᣖฬ߇ᄢ㊁ၔᏒౝߩ㘶㘩ᐫߢળ㘩ਇ᣿╳ᓟᏒౝߩኅᣖ߇⑔ጟᏒౝߢળ㘩ਇ᣿ᖚ⠪ᢙߪ৻ㇱᧂ⏕ቯࠍ฽߻
TRO㧘ಽ㑆㧕ߦࠃࠅỚ❗♖⵾ᓟ㧘࡝ࡦ࠲ࡦࠣࠬ࠹ࡦ㉄⣺Ἳࡆࡉ࡝ࠝߦࠃࠆ㘩ਛᲥߪ㧘੐଀޿ߕࠇ߽ᐔᚑᐕ

ࠍ↪޿ߡࡀࠟ࠹ࠖࡉᨴ⦡ߒ㧘㧙୚ߢ㔚ሶ㗼ᓸ߆ࠄో࿖⊛ߦჇട௑ะ߇⷗ࠄࠇߡ޿ࠆⴊᷡဳ O㧦Kࠍ

㏜ⷰኤߒߚ㧚

ේ࿃⩶ߣߒߡ޿ߚ㧚ߎࠇࠄߩᩣߪߔߴߡ⣺Ἳࡆࡉ࡝ࠝߩ

∛ේ࿃ሶߢ޽ࠆ⠴ᾲᕈṁⴊᲥ⚛㧔 TDH㧕 ࠍ↥↢ߒߚ㧚㘩ਛᲥᬌᩏ⚿ᨐᐔᚑᐕᐲߪ㧘∛ේᓸ↢‛߇ᬌ಴ߐࠇߚ੐଀ߪ੐଀
ਛ੐଀㧔%㧕ߢ޽ߞߚ㧚∛ේᓸ↢‛೎ߦ⷗ࠆߣ㧘ࡁ߹ߚ㧘੐଀ߩේ࿃㘩ຠߪ㧘੐଀߇ャ౉࠲ࠗ࡜ࠡ⽴㧘ઁߩ੐଀ߪೝりߥߤߩ฽߹ࠇࠆ઀಴ߒߢ޽ߞߚ㧚ࡠ࠙ࠗ࡞ࠬߦࠃࠆ߽ߩ߇੐଀㧔 %㧕㧘⣺Ἳࡆࡉ࡝ࠝ

ࠨ࡞ࡕࡀ࡜ߦࠃࠆ㘩ਛᲥߪ㧘੐଀޿ߕࠇ߽ⴊᷡဳ

ߦࠃࠆ߽ߩ߇੐଀㧔 %㧕㧘ࠨ࡞ࡕࡀ࡜ߦࠃࠆ߽ߩ߇

Salmonella Enteritidis ࠍ ේ࿃⩶ߣߒߡ޿ߚ㧚੐଀ߩ߁ߜ

੐଀㧔%㧕㧘࠮࡟࠙ࠬ⩶ߦࠃࠆ߽ߩ߇੐଀㧔%㧕ߢ޽੐଀ߩ S Enteritidis㧔 ᒰ⎇ⓥᚲಽ㔌ಽ㧕ߦߟ޿ߡ࿖┙ߞߚ㧚ߘߩઁߦ⣺▤಴ⴊᕈᄢ⣺⩶㧻㧘ࡆࡉ࡝ࠝ࡮ࡒ

ᗵᨴ∝⎇ⓥᚲߦࡈࠔ࡯ࠫဳ೎㧔 PT ࠍଐ㗬ߒߚ⚿ᨐ㧘ࠨࡒࠞࠬ㧘㤛⦡ࡉ࠼࠙⃿⩶෸߮ࡁࡠ࠙ࠗ࡞ࠬߣਅ∯ේᕈᄢࡦ࠼ࠗ࠶࠴ߩ੐଀ߩ S Enteritidis ߪ PTߢ㧘ේ࿃㘩ຠ⣺⩶ߣߩᷙวᗵᨴߦࠃࠆ㘩ਛᲥ㧔⇼޿ࠍ฽߻㧕߇ฦ੐߇್᣿ߒߥ߆ߞߚ੐଀ಽߪ PT㨎ߢ޽ߞߚ㧚଀޽ߞߚ㧔⴫㧘࿑㧝 㧕㧚ߒ߆ߒ㧘ේ࿃‛⾰߇․ቯߢ߈ߥ

ࡁࡠ࠙ࠗ࡞ࠬߦࠃࠆ㘩ਛᲥߪ㧘᦬߅ࠃ߮㧙᦬ߦ⊒޿੐଀߇੐଀㧔㧑㧕ߢ㧘៝౉ᬌᩏ᧚ᢱߩ♖ᩏ߿ᬌᩏ↢ߒߚ੐଀ߢ⹺߼ࠄࠇߚ 㧚ࡁࡠ࠙ࠗ࡞ࠬߪㆮવሶဳߢ㧘

ᣇᴺߦߟ޿ߡ߽੹ᓟᬌ⸛ߔߴ߈ߢ޽ࠆߣ⠨߃ࠄࠇߚ㧚ࠣ࡞࡯ࡊΣ㧔G Σ㧕ߣࠣ࡞࡯ࡊΤ㧔G Τ㧕ߦಽߌࠆߎߣ ߇ߢ߈ࠆ㧚੹ᐕᐲߩࡁࡠ࠙ࠗ࡞ࠬߩㆮવሶဳߪ㧘Ⴎၮ㈩࠙ࠗ࡞ࠬ෸߮ᄢ⣺⩶ Oa㧔 aggR ଻᦭㧕ߪᬌ಴ߐࠇߥ߆

೉ࠍ⸃ᨆߒߚ⚿ᨐ㧘੐଀߇ G Τන⁛㧘੐଀߇ G Σනߞߚ㧚㑐ଥ⥄ᴦ૕ߩදജࠍᓧߡ㧘ᖚ⠪ฬ෸߮ᣉ⸳ᓥ੐⁛ 㧘੐଀߇ G Σ෸߮ G Τ ߢ޽ߞߚ㧚⠪ฬ߆ࠄᬌ಴ߐࠇߚᄢ⣺⩶ Oa㧔 aggR ଻᦭㧕ᩣߦ㧠ߟ޿ߡ㧘ࡄ࡞ࠬࡈࠖ࡯࡞࠼ࠥ࡞㔚᳇ᵒേᴺߦࠃࠆ DNA੐଀⚫੺ᐔᚑᐕᐲߪ㧘᦬߆ࠄ᦬ห৻ᣉ⸳ߢኋᴱߥ޿ߒ㘩੐⸃ᨆࠍⴕߥߞߚ㧚ߘߩ⚿ᨐ㧘ᣉ⸳㑐ଥ⠪ฬ෸߮ᖚ⠪ฬ߿౉ᶎࠍߒߚߎߣࠍ౒ㅢߣߒߚ਻Ꮊ⋵ߦ߹ߚ߇ࠆᄢⷙ

ࠍ㒰ߊฬ߆ࠄᬌ಴ߐࠇߚᄢ⣺⩶ Oa㧔 aggR ଻᦭㧕ᩣᮨ㘩ਛᲥ⇼޿੐଀߇⊒↢ߒߚ㧚ᐔᚑᐕ᦬ᣣߦ╳ᓟߪ㧘㧑ߩㄭૃᐲࠍ␜ߒ㧚ߎࠇࠄᩣߪห৻ߩ↱᧪ߢᏒᣇ㕙ࠍᣏⴕߒߚ㐳ፒᏒߩㄘදࠣ࡞࡯ࡊฬਛฬ߇

޽ࠆߎߣ߇ᒝߊ␜ໂߐࠇߚ㧚ߎࠇࠄߩߎߣ߆ࠄᧄ੐଀ߩ

ᣣ߆ࠄ⣻∩㧘ཌྷฯ㧘ਅ∯ߩ∝⁁ࠍ๒ߒߚᣦ㧘᦬ᣣ㐳ේ࿃‛⾰ߪ 㧘ࡁࡠ࠙ࠗ࡞ࠬ෸߮ᄢ⣺⩶ Oa㧔aggR ଻᦭ 㧕ፒ⋵ߩ▤ロ଻ஜᚲߦዯ಴߇޽ࠅ㧘㐳ፒ⋵߆ࠄᒰ⋵↢ᵴⴡߢ޽ࠆߣ⠨߃ࠄࠇߚ߇㧘ේ࿃ߦߟ޿ߡߪ․ቯߢ߈ߥ߆ߞ

↢⺖ߦ⺞ᩏଐ㗬߇޽ߞߚ㧚⑔ጟ⋵ߢߪ࿅૕߇┙ߜነߞߡߚ㧚㘶㘩ߒߚᣉ⸳ A ෸߮ᣉ⸳ B ߦߟ޿ߡ㑐ㅪ⺞ᩏࠍߒߚ߇ 㧘ࡁࡠ࠙ࠗ࡞ࠬ෸߮㘩ਛᲥ⚦⩶ߪᬌ಴ߐࠇߥ߆ߞߚ㧚৻ᣇ 㧘หᐕ᦬ᣣ㧘ർ਻ᎺᏒ߆ࠄᒰ⋵ߦ㧘ᣉ⸳ B ߦኋᴱߒߚ

ਇ᣿㪊㪉㩼

ዋᐕ㊁⃿࠴࡯ࡓ㑐ଥ⠪߇㧘ཌྷฯ㧘ਅ∯㧘⣻∩ߩ∝⁁ࠍ๒䊉䊨䉡䉟䊦䉴
㪉㪎㩼ߒߡ∛㒮ࠍฃ⸻ߒߡ޿ࠆᣦߩㅪ⛊߇޽ߞߚ㧚ߘߩᓟߩ⺞ᩏߢ᦬߆ࠄ᦬਄ᣨߦ߅ߌࠆᣉ⸳ B ߢߩኋᴱ⠪ߦཌྷฯ㧘ਅ∯㧘⣻∩ࠍਥᓽߣߒߚ㘩ਛᲥ᭽ߩᖚ⠪߇⊒↢ߒߡ޿ࠆ
ᷙวᗵᨴ
㪊㩼
㪈㪌㪎
㪊㩼

ߎߣ߇ಽ߆ߞߚ㧚ᖚ⠪ߪ㧘⑔ጟ⋵㧘㐳ፒ⋵㧘૒⾐⋵㧘ችፒ⋵ߥߤᐢၞߦࠊߚߞߡ⊒↢ߒߡ޿ߚ㧚ᖚ⠪㧘ᣉ⸳ᓥᬺຬߩଢ㧘㘩ຠ㧘᳓෸߮᜞߈ขࠅ᧚ᢱߦߟ޿ߡ㧘⑔ጟ⋵ࠍ䈸䈓㪊㩼⣺Ἳ䊎䊑䊥䉥
㪈㪋㩼䊎䊑䊥䉥䊶䊚䊚䉦䉴䉰䊦䊝䊈䊤㤛⦡䊑䊄䉡⃿⩶
㪊㩼
㪏㩼
䉶䊧䉡䉴⩶
㪊㩼
㪌㩼

ᐔᚑ㪈㪍ᐕᐲ㘩ਛᲥ䈮䈍䈔䉎ฦ∛࿃‛⾰䈱ഀว฽߼ߚ㑐ଥ⥄ᴦ૕ߢ㘩ਛᲥ⚦⩶ᬌᩏ෸߮ࡁࡠ࠙ࠗ࡞ࠬߩ
ᬌᩏࠍታᣉߒߚ㧚ઁ⥄ᴦ૕ߢᬌᩏߒߚᖚ⠪ଢ߆ࠄࡁࡠ࠙
ࠗ࡞ࠬ㧔G Σߩߺ㧘G Τߩߺ㧘G Σ෸߮ G Τ㧕෸߮ᄢ

ᢥ₂⣺⩶ Oa㧔∛ේ࿃ሶ aggR ଻᦭㧕߇ᬌ಴ߐࠇߚ㧚ࡁࡠਛᲥ㧘ਛᄩᴺⷙ㧘㧘㧚࠙ࠗ࡞ࠬߣᄢ⣺⩶ Oa ߪ㧘ߘࠇߙࠇන⁛޽ࠆ޿ߪਔᣇ
ᬌ಴ߐࠇߚ㧚ᣉ⸳ B ߩ㑐ଥ⠪ଢ߆ࠄߪ㧘ฬ߆ࠄࡁࡠ࠙

ဈፒ೑৻㧦⚦⩶ᕈ㘩ਛᲥ㧘㘩᳓♽ᗵᨴ∝ߣ⚦⩶ᕈ㘩ࠗ࡞ࠬ㧔G Σߩߺ㧘G Τߩߺ㧕߇㧘ฬ߆ࠄᄢ⣺⩶ Oa
㧔 aggR ଻᦭㧕߇ᬌ಴ߐࠇߚ߇㧘ߘߩઁ᧚ᢱ߆ࠄߪࡁࡠ 㘩ਛᲥⴡ↢⎇ⓥળ㧦㘩ਛᲥ੐଀ߩ∉ቇ⺞ᩏࡑ࠾ࡘࠕ
࡞㧘ਛᄩᴺⷙ㧘㧚 

⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ╙ภ㧘㧙 㧘⾗ᢱᐔᚑᐕᐲ෼෰㘩ຠਛߩ㘩ਛᲥ⚦⩶ᬌᩏ᧛਄శ৻㧘㊁↰ᄙ⟤ᨑ㧘ựፒశብ㧘ၳᎹ๺⟤㧘┻ਛ㊀ᐘ㧘⍹㤥㕏ዏ⋵ౝߢᏒ⽼ߐࠇߡ޿ࠆ㘩ຠߦߟ޿ߡ㧘㘩ຠߩ቟ో⏕଻ߩ┙႐߆ࠄ㘩ਛᲥߩ੍㒐㧘ᳪᨴ㘩ຠߩឃ㒰㧘ᵹㅢ㘩ຠߩᳪᨴታᘒߩᛠីࠍ⋡⊛ߣߒߡ㘩ຠ෼෰ᬌᩏࠍⴕߞߚ㧚ᬌᩏኻ⽎ߪ‐⡺㧘⽋⡺㧘㢚⡺㧘㝼੺㘃㧘㝼੺㘃ੇ῎ຠ߅ࠃ߮㊁⩿ߩว⸘ᬌ૕ߢ޽ࠆ㧚ߘߩ⚿ᨐ㧘ᄢ⣺⩶⟲ઙ㧘㤛⦡ࡉ࠼࠙⃿⩶ઙ㧘࠮࡟࠙ࠬ⩶ઙ㧘ࠨ࡞ࡕࡀ࡜ઙ㧘ࠞࡦࡇࡠࡃࠢ࠲࡯ઙ߇ᬌ಴ߐࠇߚ㧚ߐࠄߦ㧘ᒰᐕᐲߪ㧘↢㘩↪ࠞࠠઙࠍ㧘৻⥸⚦⩶ᢙ㧘ᄢ⣺⩶ᦨ⏕ᢙ㧘⣺Ἳࡆࡉ࡝ࠝ߅ࠃ߮⿒∯⩶ߦߟ޿ߡᬌᩏࠍⴕߞߚ㧚ߘߩ⚿ᨐ㧘޿ߕࠇߩᬌ૕߆ࠄ߽⣺Ἳࡆࡉ࡝ࠝ߅ࠃ߮⿒∯⩶ߪᬌ಴ߐࠇߥ߆ߞߚ㧚߹ߚ㧘⇓᳓↥㘩ຠߦߟ޿ߡߪ㧘ᱷ⇐᛫↢‛⾰ࡕ࠾࠲࡝ࡦࠣᬌᩏ߽૬ߖߡⴕߞߚ㧚ߘߩ⚿ᨐ㧘޿ߕࠇߩᬌ૕߆ࠄ߽ᱷ⇐᛫↢‛⾰ߪᬌ಴ߐࠇߥ߆ߞߚ㧚㨇ࠠ࡯ࡢ࡯࠼㧦෼෰ᬌᩏ㧘㘩ຠᬌᩏ㧘⚦⩶㨉㧝⇓᳓↥㘩ຠߦߟ޿ߡ㧘ᱷ⇐᛫↢‛⾰ߩ᦭ήࠍ⺞ᩏߒߚ㧚ߪߓ߼ߦ⑔ጟ⋵ߢߪ㧘⋵᡽ߩ዁᧪ᜰ㊎ߢ޽ࠆ‫߆߅ߊ߰ޟ‬ᣂ਎♿

⸘↹‫ߩޠ‬ᐨᢥߦ㧘੹ߩᤨઍߦߟ޿ߡ‫⋵ޟ‬᳃ߩ↢ᵴ᭽ᑼߩ㧞

ᣇᴺᄌൻ߿ᔒะߩᄙ᭽ൻߥߤ㧘ᄢ߈ߥᤨઍߩォ឵ᦼߦ⋥㕙ߒ

㧞࡮㧝

ᬌᩏ㗄⋡

ߡ‫ࠆ޿ߡߒߣࠆ޿ޠ‬㧚↢ᵴ᭽ᑼߪ㧘ᣣ‫ޘ‬ᄙ᭽ൻߒ㧘ߘߩᬌᩏ㗄⋡ߪ㧘ᳪᨴᜰᮡ⚦⩶㧔৻⥸⚦⩶ᢙ㧘ᄢ⣺⩶⟲㧘ਛߢ߽㘩ᢥൻߩᄙ᭽ൻߪ㧘ߘߩઍ⴫ᩰߢ޽ࠆߣ⠨߃ࠇࠆ 㧚ህ᳇ᕈ⚦⩶ᢙ㧕߅ࠃ߮㘩ਛᲥ⚦⩶㧔㤛⦡ࡉ࠼࠙⃿⩶㧘ࠨᄖ㘩↥ᬺߩ⊒ዷߣ㧘ャ౉㘩ᢱߩჇടߥߤ߇㧘ߘߩⷐ࿃ߣ

࡞ࡕࡀ࡜ 㧘⣺▤಴ⴊᕈᄢ⣺⩶ O㧘ࠞࡦࡇࡠࡃࠢ࠲࡯ 㧘⠨߃ࠄࠇࠆ߇㧘ߎߩࠃ߁ߥ⁁ᴫߩਛ㧘࿖ߢߪ㘩ຠ቟ోᆔࠛ࡞ࠪ࠾ࠕ 㧘࠙ࠛ࡞ࠪࡘ⩶ 㧘࠮࡟࠙ࠬ⩶㧘࠽ࠣࡆࡉ࡝ࠝ㧘ຬળߩ⸳⟎╬ࠍᩇߣߒߚ㘩ຠ቟ోၮᧄᴺ㧔ᐔᚑᐕᴺᓞ

⣺Ἳࡆࡉ࡝ࠝ㧘ࡆࡉ࡝ࠝ࡮ࡒࡒࠞࠬ㧘ࡆࡉ࡝ࠝ࡮ࡈ࡞ࡆ

╙ภ㧕ࠍ౏Ꮣߒ㧘ᣂᤨઍߦ߅ߌࠆ㘩ຠߩ቟ోᕈߩ⏕଻ࠕ࡝ࠬ㧕ߩ㗄⋡ߦߟ޿ߡᬌᩏߒߚ㧚߹ߚ㧘ᒰᐕᐲߪ㧘ࠍ࿑ߞߡ޿ࠆ㧚⋵ߢ߽㧘ߎߩᴺᓞߦ๭ᔕߒߡߐ߹ߑ߹ߥ

↢㘩↪ࠞࠠߦߟ޿ߡ㧘৻⥸⚦⩶ᢙ㧘ᄢ⣺⩶ᦨ⏕ᢙ㧘⣺Ἳᣉ╷ࠍታᣉߒߡ޿ࠆ㧚㘩ຠߩ቟ో⏕଻ߦߟ޿ߡߪ㧘㘩ਛࡆࡉ࡝ࠝ߅ࠃ߮⿒∯⩶ߩ㗄⋡ߦߟ޿ߡᬌᩏࠍⴕߞߚ㧚Ქ㧘㘩ຠᷝട‛㧘㘩ຠᱷ⇐ㄘ⮎㧘㘩ຠਛߩൻቇ‛⾰㧔᳓

㧞࡮㧞

ᬌ૕

㌁㧘ࠞ࠼ࡒ࠙ࡓ㧘࠳ࠗࠝࠠࠪࡦߥߤ 㧕㧘ౕེ࡮ኈེ൮ⵝ

ᐔᚑᐕ᦬߆ࠄ᦬ߦ߆ߌߡ㧘⋵↢ᵴⴡ↢⺖ࠍㅢߓ࡮߅߽ߜ߾࡮ᵞᵺ೷㧘‐ᶏ✎⁁⣖∝㧘ㆮવሶ⚵ߺ឵߃㘩⋵ౝ଻ஜ⑔␩ⅣႺ੐ോᚲߢ෼෰ߒߚ‐⡺ઙ㧘⽋⡺ຠ㧘ஜᐽ㘩ຠ㧘㘩ຠ⴫␜㧘ャ౉㘩ຠ⋙ⷞ㧘ߘߩઁ㧔㜞∛ઙ㧘㢚⡺ઙ㧘㝼੺㘃ઙ㧘㝼੺㘃ੇ῎ຠઙ㧘㊁⩿ઙේᕈ㠽ࠗࡦࡈ࡞ࠛࡦࠩߥߤ㧕ߩ⻉㗄⋡ߦᄢ೎ߐࠇࠆ߇㧘߅ࠃ߮↢㘩↪ࠞࠠઙว⸘ᬌ૕ߦߟ޿ߡᬌᩏߒߚ㧚㘩ਛᲥߪߘߩ⊒↢㗫ᐲ߆ࠄ⠨߃ߡ߽㧘㘩ຠⴡ↢ᣉ╷ߩᩇ㧞࡮㧟⚦⩶ᬌᩏᣇᴺߣߥࠆ߽ߩߣ⠨߃ࠄࠇࠆ㧚ᒰ⺖ߢ߽㧘㘩ຠߩ቟ో⏕଻ߩߘࠇߙࠇߩ㘩ຠߦߟ޿ߡฦ㗄⋡ߩᬌᩏᣇᴺߪ㧘ᚑಽⷙਛߢ㧘㘩ਛᲥ੍㒐ߩⷰὐ߆ࠄ㧘㘩ຠਛߩ㘩ਛᲥ⚦⩶޽ࠆᩰ߇޽ࠆ㘩ຠߪ౏ቯᴺ㧔㘩ຠⴡ↢ᴺ߅ࠃ߮㑐ㅪᴺⷙ㧕 ޿ߪ㧘ߘߩᜰᮡߣߥࠆᳪᨴᜰᮡ⩶ߩᬌᩏߦജࠍ౉ࠇߡ޿

ߦᓥ޿ታᣉߒ㧘ߘࠇએᄖߩ㘩ຠߦ㑐ߒߡߪ㧘㘩ຠⴡ↢ᬌࠆ㧚ߎߩࠃ߁ߥ⁁ᴫߩਛ㧘ᒰᐕᐲߪ㧘㘩ຠⴡ↢⋙ⷞຬ߇ᩏᜰ㊎ ߅ࠃ߮ᬌᩏ⺖ᓸ↢‛ࡑ࠾ࡘࠕ࡞ ߅ࠃ߮⑔ጟ⋵଻෼෰ߒߚ㘩ຠߦߟ޿ߡ㧘ᳪᨴᜰᮡ⚦⩶߿㘩ਛᲥ⚦⩶ߩᬌஜⅣႺ⎇ⓥᚲ∛ℂ⚦⩶⺖㘩ຠᬌᩏᬺോ▤ℂࡑ࠾ࡘࠕ࡞ߦ

ᩏࠍⴕߞߚ㧚߹ߚ㧘ෘ↢ഭ௛⋭ක⮎ዪ㘩ຠ଻ஜㇱ⋙ⷞ቟ᓥߞߚ㧚ో⺖㐳ㅢ⍮㧔ᐔᚑᐕ᦬ᣣ 㧘㘩⋙⊒╙ภ 㧕ߦࠃࠅ㧘⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲ

㧔‫ޥ‬- ⑔ጟ⋵ᄥቿᐭᏒᄢሼะ૒㊁㧕 ࠛ࡞ࠪ࠾ࠕ㧘ࠞࡦࡇࡠࡃࠢ࠲࡯㧘㤛⦡ࡉ࠼࠙⃿⩶㧘ࡆࡉ㧟⚿ᨐ࡝ࠝዻ㧘࠮࡟࠙ࠬ⩶ߩᬌᩏᣇᴺߪ㧘ᬌ૕ g ߦ࡝ࡦ㉄㧟࡮㧝⚦⩶ᬌᩏ⚿ᨐ

✭ⴣࡍࡊ࠻ࡦ᳓ࠍ ml ട߃ࠬ࠻ࡑ࠶ࠠࡦࠣߒ㧘ࠛ࡞⚦⩶ᬌᩏ⚿ᨐࠍ⴫ߦ␜ߔ㧚ᄢ⣺⩶⟲ߪઙ߇㓁ᕈࠍ

ࠪ࠾ࠕჇ⩶ၭ࿾㧘ࡊ࡟ࠬ࠻ࡦၭ࿾㧘ࠕ࡞ࠞ࡝ࡍࡊ࠻ࡦ㧘␜ߒ㧘㤛⦡ࡉ࠼࠙⃿⩶ߪઙ߆ࠄᬌ಴ߐࠇߚ㧚߹ߚ㧘㢚ࡐ࡝ࡒࠠࠪࡦ B ߅ࠃ߮%Ⴎൻ࠽࠻࡝࠙ࡓࡉ࡛ࠗࡦߢ

⡺ઙ߆ࠄࠞࡦࡇࡠࡃࠢ࠲࡯߇ᬌ಴ߐࠇ߁ߜઙߪ

Ⴧ⩶ၭ㙃ߒߚᓟ 㧘CIN ኙᄤၭ࿾ 㧘ࠬࠠࡠ࡯ኙᄤၭ࿾ 㧘TCBS

Campylobacter jejuni ߩ ߺ㧘߽߁৻ઙ߆ࠄߪ C jejuni ෸ኙᄤၭ࿾㧘 NGKG ኙ ᄤၭ࿾㧘ࡆࡉ࡝ࠝኙᄤ߅ࠃ߮ࠛ࠶

߮ C coli ߇ᬌ಴ߐࠇߚ㧚ߘߩⴊᷡဳߪ㧘Penner ߩ D ෸࡛ࠣ࡯ࠢኙᄤၭ࿾ߥߤߩಽ㔌ၭ࿾ߢᬌ಴ߒߚ㧚ᬌᩏኻ⽎߮ဳ೎ਇ⢻ߢ޽ߞߚ㧚࠮࡟࠙ࠬ⩶ߪ⽋⡺ઙ㧘㢚⡺ઙ㧘ߣ⠨߃ࠄࠇࠆࠦࡠ࠾࡯ࠍ㊒⩶ߒ㧘TSI ኙᄤၭ࿾߿ SIM ኙ㝼੺㘃ઙ㧘㊁⩿ઙ㧘ว⸘ઙ߆ࠄᬌ಴ߐࠇߚ㧚ࠨ࡞ࡕᄤၭ࿾╬ߢ↢ൻቇᕈ⁁ࠍ⏕⹺ߒߚ㧚ᔅⷐߦᔕߓߡⴊᷡဳ

ࡀ࡜ߪ㢚⡺ઙ߆ࠄᬌ಴ߐࠇ 㧘Salmonella ⴊ ᷡဳ Infantis೎⹜㛎߿ઁߩ⚦⩶ቇ⊛ᬌᩏࠍⴕ޿หቯߒߚ㧚⣺▤಴ⴊᕈ߇ઙ㧘Salmonella ⴊ ᷡဳᄢ⣺⩶ Oߩᬌᩏᣇᴺߪ㧘ᬌ૕ g ߦࡁࡏࡆࠝࠪࡦടⴊ ᷡ ဳ Manhattan ߇  ઙ 㧘 ෸ ߮ Salmonella ⴊ ᷡ ဳ

ᄌ ᴺ EC ၭ ࿾ 㧔 modified Escherichia

coli broth withEnteritidis ߇ ઙᬌ಴ߐࠇߚ㧚࠙ࠛ࡞ࠪࡘ⩶ߪ 㧘㢚⡺ઙ 㧘

Yovokome ߇ ઙ㧘Salmonella

noboviocin㧘N-mEC㧕ࠍ ml ട߃ࠬ࠻ࡑ࠶ࠠࡦࠣߒߚ㧚㝼੺㘃ઙ෸߮㝼੺㘃ੇ῎ຠઙ߆ࠄᬌ಴ߐࠇߚ㧚ోߡߩ͠ߢᤨ㑆ၭ㙃ᓟ㧘఺∉⏛᳇ࡆ࡯࠭ߢ⣺▤಴ⴊᕈᄢ⣺↢㘩ߩࠞࠠ߆ࠄ⿒∯⩶߅ࠃ߮⣺Ἳࡆࡉ࡝ࠝߪ㧘ᬌ಴ߐࠇ⩶ Oࠍ㓸⩶ߒߚ㧚CHROMagar O ኙᄤၭ࿾߅ࠃ߮

ߥ߆ߞߚ㧚

cefixime㧘 ੝࠹࡞࡞㉄ࠞ࡝࠙ࡓᷝട࠰࡞ࡆ࠻࡯࡞ࡑ࠶ࠦ

㧟࡮㧞޿ߕࠇߩᬌ૕߆ࠄ߽ᱷ⇐᛫↢‛⾰╬ߪᬌ಴ߐࠇߥ߆ߞ

ࡦࠠ࡯ኙᄤၭ࿾ߢᬌ಴ߒߚ㧚ᬌᩏኻ⽎ߣ⠨߃ࠄࠇࠆࠦࡠ
࠾࡯ࠍ㊒⩶ߒ TSI ኙᄤၭ࿾㧘SIM ኙᄤၭ࿾෸߮࡝ࠪࡦ⣕⇓᳓↥㘩ຠߩᱷ⇐‛⾰ࡕ࠾࠲࡝ࡦࠣᬌᩏ⚿ᨐߚ㧚὇㉄⏕⹺ၭ࿾╬ߢ↢ൻቇᕈ⁁ࠍ⏕⹺ߒߚ㧚ᔅⷐߦᔕߓߡ
ⴊᷡဳ೎⹜㛎߿ᔒ⾐Ქ⚛↥↢⹜㛎ࠍⴕ޿หቯߒߚ㧚ࠨ࡞㧠⠨ኤࡕࡀ࡜ߩᬌᩏᣇᴺߪ㧘ᬌ૕ g ߦ Buffered peptone water㘩ຠߏߣߩᄢ⣺⩶⟲ߩᬌ಴₸ࠍᲧセߔࠆߣ㧘㊁⩿෸߮㧔 BPW㧕ࠍ ml ട߃ࠬ࠻ࡑ࠶ࠠࡦࠣߒ㧘͠ߢᤨ

㢚⡺߇% ߣᦨ߽㜞ߊ㧘㝼੺㘃߇%ߢ޽ߞߚ㧚㤛㑆ၭ㙃ᓟ㧘Rappaport - Vasssiliadis ࠨ ࡞ࡕࡀ࡜Ⴧ⩶ࡉࠗ

⦡ࡉ࠼࠙⃿⩶ߦߟ޿ߡߪ㧘㝼੺㘃߇%ߣ㜞ߊ㧘࠮࡟࡛ࡦ߅ࠃ߮࠹࠻࡜࠴ࠝࡦ㉄Ⴎၭ࿾ߢၭ㙃ߒ㧘 XLTኙᄤ

࠙ࠬ⩶ߦߟ޿ߡߪ㧘㊁⩿ߢ%ߢ޽ߞߚ㧚ࠨ࡞ࡕࡀ࡜

ၭ࿾߅ࠃ߮ SM-ID ኙ ᄤၭ࿾ߢᬌ಴ߒߚ㧚ᬌᩏኻ⽎ߣ⠨߅ࠃ߮ࠞࡦࡇࡠࡃࠢ࠲࡯ߦߟ޿ߡߪ㧘㢚⡺ߩߺ߆ࠄ

߃ࠄࠇࠆࠦࡠ࠾࡯ࠍ㊒⩶ߒ㧘 TSI ኙᄤၭ࿾SIM ኙᄤၭ%߅ࠃ߮ %ߩᬌ಴₸ߢ޽ࠅᵈᗧࠍⷐߔࠆ㧚ㆊ෰ߩᧄ࿾߅ࠃ߮࡝ࠪࡦ⣕὇㉄⏕⹺ၭ࿾╬ߢ↢ൻቇᕈ⁁ࠍ⏕⹺ߒ⺞ᩏߦ߅ߌࠆ㢚⡺ߩࠨ࡞ࡕࡀ࡜߅ࠃ߮ࠞࡦࡇࡠࡃࠢ࠲࡯

ߚ㧚ᔅⷐߦᔕߓߡⴊᷡဳ೎⹜㛎߿ઁߩ⚦⩶ቇ⊛ᬌᩏࠍߩᬌ಴⁁ᴫ ࠍᲧセߔࠆߣ㧘ᐔᚑᐕᐲߪࠨ࡞ࡕࡀ࡜߇ⴕ ޿ ห ቯ ߒ ߚ 㧚⿒∯⩶ߩᬌᩏᣇᴺߪᬌ૕ g ߦ BPW

%㧘ࠞࡦࡇࡠࡃࠢ࠲࡯߇%ᬌ಴ߐࠇ㧘ᐔᚑᐕᐲ

ࠍ ml ട߃ࠬ࠻ࡑ࠶ࠠࡦࠣߒ㧘͠ߢᤨ㑆ᅢ᳇⊛ߪ㧘ࠨ࡞ࡕࡀ࡜߇%ᬌ಴ߐࠇߚ㧚ᒰᐕᐲߩᬌ಴₸ߣߦၭ㙃ߒ㧘ࡁࡏࡆࠝࠪࡦട Shigella broth ߦ ធ⒳ߒ͠

Ყセߔࠆߣ㧘ਔ⩶⒳ߣ߽ᄢ߈ߥᄌൻߪߥ޿߇㧘ࠞࡦࡇࡠߢᤨ㑆ህ᳇⊛ߦၭ㙃ߒߚ㧚 DHL ኙᄤၭ࿾㧘SS ኙᄤၭࡃࠢ࠲࡯෸߮ࠨ࡞ࡕࡀ࡜ߪ㧘⚦⩶ᕈ㘩ਛᲥߩᦨ߽ᄙ޿∛࿾߅ࠃ߮ CHROMagar O TAM ߢᬌ಴ߒ㧘ᔅⷐߦᔕߓߡ

࿃‛⾰ߢ޽ࠅ㧘㢚⡺ࠍᛒ߁㘶㘩ᐫ㧘ዊᄁᬺ⠪㧘ᶖ⾌⠪߳↢ൻቇᕈ⁁ߩ⏕⹺ࠍⴕߞߚ㧚ߩᵈᗧ༐⿠߇ᔅⷐߣ⠨߃ࠄࠇࠆ㧚㝼੺㘃ߦߟ޿ߡߪ⣺Ἳࡆࡉ࡝ࠝᬌᩏߦᦧ߃ߡ㧘ෘ↢ഭ

ᳪᨴᜰᮡ⩶ߢ޽ࠆᄢ⣺⩶⟲ߪ㧘߶ߣࠎߤߩ㘩ຠ߆ࠄᬌ௛⋭ක⮎ዪ㘩ຠ଻ஜㇱၮḰ⺖㐳ㅢ⍮㧔ᐔᚑᐕ᦬ᣣ 㧘಴ߐࠇߡ߅ࠅ㧘ᵹㅢㆊ⒟ߢ૗ࠄ߆ߩᳪᨴ߇↢ߓߚน⢻ᕈ㘩ၮ⊒╙ภ㧕ߦࠃࠅ㧘⣺Ἳࡆࡉ࡝ࠝ⩶ᢙࠍ᷹ቯߔࠆᦨ

߇޽ࠆ㧚․ߦ㧘੹࿁⺞ᩏࠍⴕߞߚ㊁⩿߆ࠄߩᄢ⣺⩶⟲ߩ⏕ᢙᬌᩏࠍታᣉߒߚ㧚߹ߚ㧘ᳪᨴᜰᮡ⚦⩶ߩᬌᩏߪ㧘౏ᬌ಴₸ߪᦨ߽㜞ߊ㧘㘩ߔࠆ೨ߦᵹ᳓ߢᵞᵺߒ㧘㐳ᤨ㑆ቶ

ቯᴺߦࠃࠅⴕߞߚ㧚᷷ߦ᡼⟎ߒߥ޿╬ߘߩขࠅᛒ޿ߦᵈᗧߔࠆߎߣ߇ᦸ߹ߒ

㧞࡮㧠޿ߣ⠨߃ࠄࠇࠆ㧚⇓᳓↥㘩ຠߩᱷ⇐‛⾰ࡕ࠾࠲࡝ࡦࠣᬌᩏᣇᴺ‐⡺ઙ㧘⽋⡺ઙ㧘㝼੺㘃ઙߩ㧘ว⸘ઙߦߟ޿

ᐔᚑᐕᐲ߆ࠄ⺞ᩏߒߚ↢㘩↪ࠞࠠ߆ࠄߪ㧘⿒∯⩶߇ߡ㧘ᱷ⇐᛫↢‛⾰╬㧔ࡍ࠾ࠪ࡝ࡦ♽㧘ࠕࡒࡁࠣ࡝ࠦࠪ࠼

ᬌ಴ߐࠇߥ߆ߞߚ 㧚៝౉ߐࠇߚࠞࠠߪ࿖ౝ↥ߢ޽ࠆߚ߼㧘♽㧘ࡑࠢࡠ࡜ࠗ࠼♽㧘࠹࠻࡜ࠨࠗࠢ࡝ࡦ♽㧘ࠢࡠ࡜ࡓࡈ

࿖ౝ↥ߩࠞࠠߦߟ޿ߡ⿒∯⩶ߩᳪᨴߪ㧘⃻࿷ߩᚲή޿ߣ

ࠚ࠾ࠦ࡯࡞㧘ࡁࡏࡆࠝࠪࡦ㧘ࡈࡑ࡞㉄࠴ࠕࡓ࡝ࡦ㧕ߩ᦭⠨߃ࠄࠇࠆ㧚߹ߚ㧘৻⥸⚦⩶ᢙ㧘ᄢ⣺⩶ᢙ౒ߦઁߩ㘩ຠήࠍ㧘ᓸ↢‛ࠍ↪޿ߚ◲ᤃᬌᩏᴺߦࠃࠅᬌᩏߒߚ㧚ߣᲧセߒߡૐߊ㧘ߘߩᵹㅢ⚻〝ߢ߽⚦⩶ቇ⊛ߥᳪᨴߪή ޿߽ߩߣ⠨߃ࠄࠇࠆ㧚ᧄ⋵ߩၮᧄ᭴ᗐߢ޽ࠆ‫ޟ‬ஜ߿߆ߢߣ⠨߃ࠄࠇࠆ㧚ᔃ⼾߆ߥ⋵᳃↢ᵴߩ⏕଻‫ࠍޠ‬ታ⃻ߒᚢ⇛⊛ࡊࡠࠫࠚࠢ࠻

ᢥ₂ߩᩇߩ৻ߟߢ޽ࠆ‫౒␩⑔ޟ‬ഃ┙⋵ ‫ࠍޠ‬ታ⃻ߔࠆߚ߼ߦ߽㧘ෘ↢⋭⋙ୃ㧦㘩ຠⴡ↢ዊ౐ᴺ㧘ᐔᚑᐕᐲ
㧘᧲੩㧦ᣂᣣᧄᴺⷙ㧘㧚㘩ຠⴡ↢ⴕ᡽ߦ⽸₂ߔࠆᬌᩏߩ޽ࠅᣇߣ࠺࡯࠲ߩࡈࠖ࡯
࠼ࡃ࠶ࠢ߇ᔅⷐߢ޽ࠆߣ⠨߃ࠄࠇࠆ㧚㧘ෘ↢⋭⋙ୃ㧦㘩ຠⴡ↢ᬌᩏᜰ㊎࡮ᓸ↢‛✬㧘-㧘᧲੩㧘ᣣᧄ౏ⴐⴡ↢දળ㧘㧚

㧡



߹ߣ߼

⑔ጟ⋵଻ஜ⑔␩ㇱ㧦ᬌᩏ⺖ᓸ↢‛ࡑ࠾ࡘࠕ࡞㧘-㧘⑔ጟ㧘㧚੹࿁ߩ⺞ᩏߢ 㧘⃻࿷ᵹㅢߒߡ޿ࠆ㘩ຠਛߩ㘩ਛᲥ⚦⩶ 㧘ᱷ⇐᛫↢‛⾰╬ߩᳪᨴ⁁ᴫࠍᛠីߔࠆߎߣ߇ߢ߈ߚ㧚ߎ⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲ㧦⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ㧘㧘-㧘⑔ጟ㧘㧚ࠇࠄߩ࠺࡯࠲ߪ㧘੹ᓟ㧘㘩ਛᲥ੍㒐ኻ╷߅ࠃ߮㘩ຠߩ቟ోᕈ⏕଻ߩߚ߼ߦ೑↪ߢ߈㧘㘩ຠⴡ↢ⴕ᡽ߦᓎ┙ߟ߽ߩ⴫ ᳪᨴᜰᮡ⚦⩶޽ࠆ޿ߪ㘩ਛᲥ⚦⩶߇ᬌ಴ߐࠇߚᬌ૕ᢙ

ᬌᩏઙᢙ ᄢ⣺⩶⟲ 㤛 ⦡ ࡉ ࠼ ࠞࡦࡇࡠ ⣺Ἳࡆࡉ ࠮࡟࠙ࠬ⩶ ࠙ ࠛ ࡞ ࠪ ࠨ࡞ࡕࡀ࡜
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ᬌᩏߖߕ

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ᬌᩏߖߕ

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ᬌᩏߖߕ

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





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↢㘩↪ࠞࠠ



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⸘





ᬌᩏߖߕ ᬌᩏߖߕ




 

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

ᬌᩏߖߕ

 

⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ╙ภ㧘㧙㧘⾗ᢱᐔᚑᐕᐲ㘩ຠߩ㘩ਛᲥ⩶ᳪᨴታᘒ⺞ᩏựፒశብ㧘᧛਄శ৻㧘ၳᎹ๺⟤㧘㊁↰ᄙ⟤ᨑ㧘┻ਛ㊀ᐘ㧘⍹㤥㕏ዏ৻⥸ߦᏒ⽼ߐࠇߡ޿ࠆ㘩ຠߦߟ޿ߡ㧘㘩ਛᲥߩ੍㒐㧘ᳪᨴ㘩ຠߩឃ㒰㧘ᵹㅢ㘩ຠߩᳪᨴታᘒߩᛠី
ࠍ⋡⊛ߣߔࠆ㘩ਛᲥ⩶ߩᳪᨴታᘒ⺞ᩏࠍⴕߞߚ㧚㊁⩿㧘ࠬ࠹࡯ࠠ↪‐⡺㧘ࡒࡦ࠴⡺߅ࠃ߮↢㘩↪⡺ߩว⸘ᬌ૕ߦߟ޿ߡ㧘ᄢ⣺⩶㧘ࠨ࡞ࡕࡀ࡜߅ࠃ߮⣺▤಴ⴊᕈᄢ⣺⩶ Oߩᬌᩏࠍⴕߞߚ㧚ߘߩ⚿ᨐ㧘ᄢ⣺⩶ઙ߅ࠃ߮ࠨ࡞ࡕࡀ࡜ઙ߇ᬌ಴ߐࠇߚ㧚⣺▤಴ⴊᕈᄢ⣺⩶ Oߪ㧘޿ߕࠇߩᬌ૕߆ࠄ߽ᬌ಴
ߐࠇߥ߆ߞߚ㧚߹ߚ㧘↢㘩↪ࠞࠠઙߦߟ޿ߡ⿒∯⩶ߩᬌᩏࠍⴕߞߚ߇㧘޿ߕࠇߩᬌ૕߆ࠄ߽⿒∯⩶ߪᬌ಴ߐࠇߥ߆ߞߚ㧚㨇ࠠ࡯ࡢ࡯࠼ 㘩ຠᬌᩏ㧘㘩ਛᲥ⚦⩶㧘ᳪᨴታᘒ⺞ᩏ㨉㧝⩶ᢙ㧘ᄢ⣺⩶ᢙ㧕ߩᬌᩏࠍታᣉߒߚ㧚ߪߓ߼ߦㄭᐕ㧘৻⥸ߩᵹㅢ㘩ຠࠍේ࿃ߣߔࠆ㘩ਛᲥ੐ઙ߇ߒ߫

㧞࡮㧞

ᬌ૕

ߒ߫⊒↢ߒ໧㗴ߦߥߞߡ޿ࠆ㧚ߎࠇࠄߩ㘩ਛᲥߪ㧘㘩ຠᐔᚑᐕ᦬ᣣ߆ࠄ᦬ᣣߦ߆ߌߡ㧘⋵↢ᵴⴡ↢⺖ߩᵹㅢ▸࿐߇ᐢ޿ߚ߼㧘ᖚ⠪߽ᐢၞߦࠊߚߞߡ⊒↢ߒ㧘ࠍㅢߓ⋵ౝ଻ஜ⑔␩ⅣႺ੐ോᚲߢ෼෰ߒߚ㊁⩿㘃㧔ࠞᖚ⠪ᢙ߽ᄙߊߥࠆ௑ะ߇⷗ࠄࠇࠆ㧚଀߃߫㧘ᐔᚑᐕߩ

ࠗࡢ࡟㧘ࠕ࡞ࡈࠔ࡞ࡈࠔ㧘࡟࠲ࠬ㧘ߺߟ߫㧘ࡕࡗࠪ㧘ࠠੇ῎ࠗࠞ⩻ሶࠍේ࿃ߣߔࠆࠨ࡞ࡕࡀ࡜ߩ㘩ਛᲥ੐ઙ㧘ᐔࡘ࠙࡝㧘ࠞ࠶࠻㊁⩿㧕ઙ㧘ࠬ࠹࡯ࠠ↪‐⡺ઙ㧘ࡒࡦᚑᐕߩャ౉ࠞࠠࠍේ࿃ߣߔࠆ⿒∯⩶ߩ㘩ਛᲥ੐ઙߥߤ

࠴⡺ઙ㧘↢㘩↪⡺㘃ߣߒߡ‐⡺㘃ઙ㧘㢚⡺㘃ઙ㧘㚍ᄢⷙᮨߥ㘩ਛᲥ߇⊒↢ߒߡ޿ࠆ㧚ߎࠇࠄߩ੐ઙߪ㧘৻⥸⡺㘃ઙ߅ࠃ߮↢㘩↪ࠞࠠઙ㧘ว⸘ᬌ૕ߦߟ޿ߡߩᏒ⽼ᵹㅢ㘩ຠࠍේ࿃ߣߔࠆ diffuse outbreak ߢ ޽ߞߚ㧚ᬌᩏߒߚ㧚੹ᓟ㧘ߎߩࠃ߁ߥ㘩ਛᲥࠍᧂὼߦ㒐ᱛߔࠆߚ߼ߦ߽㘩ຠ

㧞࡮㧟

ᬌᩏᣇᴺ

ߘࠇߙࠇߩ㘩ຠߦߟ޿ߡฦ㗄⋡ߩᬌᩏᣇᴺߪ㧘ᚑಽⷙߩ቟ోᕈ⏕଻ߪ㧘ⴕ᡽ߩ㊀ⷐߥᓎഀߢ޽ࠆ㧚⑔ጟ⋵ߢߪ㧘㘩ਛᲥ⊒↢ߩᧂὼ㒐ᱛኻ╷㧘ᳪᨴ㘩ຠߩᩰ߇޽ࠆ㘩ຠߪ౏ቯᴺ㧔㘩ຠⴡ↢ᴺ߅ࠃ߮㑐ㅪᴺⷙ㧕 ឃ㒰㧘ᵹㅢ㘩ຠߩᳪᨴታᘒࠍᛠីࠍ⋡⊛ߣߒ㧘ᐔᚑᐕ

ߦᓥ޿ታᣉߒ㧘ߘࠇએᄖߩ㘩ຠߦ㑐ߒߡߪ㧘㘩ຠⴡ↢ᬌ᦬ᣣઃߌ㘩቟⊒╙ภෘ↢ഭ௛⋭ක⮎㘩ຠዪ㘩ᩏᜰ㊎  ߅ࠃ߮ᬌᩏ⺖ᓸ↢‛ࡑ࠾ࡘࠕ࡞  ߦᓥ޿ታᣉߒ

ຠ቟ోㇱ㐳ㅢ⍮ߦࠃࠆᐔᚑᐕᐲ㘩ຠߩ㘩ਛᲥ⩶ߩᳪᨴߚ㧚⣺▤಴ⴊᕈᄢ⣺⩶ Oߩᬌᩏߪ㧘ᬌ૕ g ߦࡁࡏታᘒ⺞ᩏߩታᣉ᭎ⷐߦၮߠ߈⺞ᩏࠍⴕߞߚ㧚ߥ߅㧘ർᶏࡆࠝࠪࡦട mEC ၭ࿾㧔modified escherichia coli broth with㆏㧘ጤᚻ⋵㧘ජ⪲⋵㧘᧲੩ㇺ㧘␹ᄹᎹ⋵㧘ᄹ⦟⋵㧘ጊญ

noboviocin㧘 એਅ N-mEC ߣ⇛ߔ㧕ࠍ ml ട߃ࠬ࠻ࡑ⋵ 㧘ችፒ⋵ 㧘ᴒ✽⋵ 㧘ᧅᏻᏒ 㧘ජ⪲Ꮢ㧘ᎹፒᏒ㧘ᮮᵿᏒ 㧘࠶ࠠࡦࠣߒߚ 㧚͠ߢᤨ㑆ၭ㙃ᓟ㧘఺∉⏛᳇ࡆ࡯࠭ߢ O

⑔ጟᏒ㧘ർ਻ᎺᏒ㧘ችፒᏒߩฦ⥄ᴦ૕ߢ߽ห᭽ߩᬌᩏࠍ   ࠍ 㓸 ⩶ ߒ 㧘 ࠢ ࡠ ࡕ ࠕ ࠟ ࡯ O   ኙ ᄤ ၭ ࿾ ߅ ࠃ ߮ⴕߞߡ޿ࠆ㧚

CT-SMAC ኙ ᄤၭ࿾ߢᬌ಴ߒߚ㧚ᬌᩏኻ⽎ߣ⠨߃ࠄࠇࠆࠦࡠ࠾࡯ࠍ㊒⩶ߒ㧘 TSI㧘 SIM㧘࡝ࠫࡦߢ↢ൻቇᕈ⁁ࠍ

㧞

ᣇᴺ

㧞㧚㧝

⏕⹺ߒߚ㧚ᔅⷐߦᔕߓߡⴊᷡဳ೎⹜㛎߿ࡌࡠᲥ⚛↥↢⹜ᬌᩏ㗄⋡㛎ࠍⴕ޿หቯߒߚ 㧚ᄢ⣺⩶ߩᬌᩏߪ 㧘ᬌ૕ g ߦ N-mEC

ᬌᩏ㗄⋡ߪ㧘ᄢ⣺⩶㧘ࠨ࡞ࡕࡀ࡜߅ࠃ߮⣺▤಴ⴊᕈᄢml ട߃㧘ࠬ࠻ࡑ࠶ࠠࡦࠣߒߚᓟ㧘͠ߢᤨ㑆ၭ㙃⣺⩶ Oߩ⩶⒳ߦߟ޿ߡⴕߞߚ㧚߹ߚ㧘ᐔᚑᐕߩャߒߚ㧚࠳࡯࡜ࡓ▤౉ࠅ EC ၭ࿾ߢᰴჇ⩶ࠍⴕ޿㧘ࠢࡠ౉ࠞࠠࠍේ࿃ߣߔࠆ⿒∯⩶ߩ㘩ਛᲥ੐ઙߩ⊒↢ࠍฃߌ㧘ࡕࠕࠟ࡯ ECC ኙᄤၭ࿾ߢᬌ಴ߒߚ㧚ࠨ࡞ࡕࡀ࡜ߩᬌᩏ

↢㘩↪ࠞࠠߦߟ޿ߡߪ㧘⿒∯⩶߅ࠃ߮ⷙᩰၮḰ㧔৻⥸⚦ߪ㧘ᬌ૕ g ߦ✭ⴣࡍࡊ࠻ࡦ᳓ࠍ ml ട߃ࠬ࠻ࡑ࠶

⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲ㧔‫ ޥ‬ᄥቿᐭᏒᄢሼะ૒㊁㧕 ࠠࡦࠣߒߚ㧚͠ߢᤨ㑆ၭ㙃ᓟ㧘࡜ࡄࡐ࡯࠻ࡉ࡛ࠗࡦ

ኤߐࠇࠆ㧚એ਄ߩ⚿ᨐ߆ࠄ㧘․ߦᄢ⣺⩶ߩᬌ಴₸߇㜞߆߅ࠃ߮ࡂ࡯࠻࠹࠻࡜࠴ࠝࡦჇ⩶ၭ࿾ߢၭ㙃ߒ㧘 XLTኙߞߚࡒࡦ࠴⡺ࠍኅᐸߢ⺞ℂߔࠆ㓙ߦߪ㧘චಽߥടᾲ߇ᔅ

ᄤၭ࿾߅ࠃ߮ SMID ኙᄤၭ࿾ߢᬌ಴ߒߚ㧚ᬌᩏኻ⽎ߣ⠨ⷐߢ޽ࠅ㧘૶↪ߔࠆ⺞ℂᯏ᧚߽ઁߩ㘩ຠߣ඙೎ߔࠆߩ߇

߃ࠄࠇࠆࠦࡠ࠾࡯ࠍ㊒⩶ߒ㧘 TSI㧘 SIM ߅ࠃ߮࡝ࠫࡦߢ

ᦸ߹ߒ޿ߣ⠨߃ࠄࠆ㧚߹ߚ㧘ߎࠇࠄߩ㘩ຠߪ㧘㐳ᤨ㑆ቶ↢ൻቇᕈ⁁ࠍ⏕⹺ߒߚ㧚ᔅⷐߦᔕߓߡⴊᷡဳ೎⹜㛎߿ઁ᷷ߦ᡼⟎ߖߕ㧘ㆡಾߥ᷷ᐲ▤ℂࠍⴕ޿଻ሽߒߥߌࠇ߫ߥ

ߩ⚦⩶ቇ⊛ᬌᩏࠍⴕ޿หቯߒߚ㧚⿒∯⩶ߩᬌᩏߪ㧘ᬌ૕ࠄߥ޿ߣ⠨߃ࠄࠇࠆ㧚 g ߦ✭ⴣࡍࡊ࠻ࡦ᳓ࠍ ml ട߃ࠬ࠻ࡑ࠶ࠠࡦࠣߒ㧘ࠨ࡞ࡕࡀ࡜߿⣺▤಴ⴊᕈᄢ⣺⩶ Oߪ㧘േ‛ߩ⣺▤͠ߢᤨ㑆ᅢ᳇⊛ߦၭ㙃ߒ㧘ࡁࡏࡆࠝࠪࡦട Shigellaౝߦ଻᦭ߐࠇߡ߅ࠅ㧘㘩⡺ಣℂߩㆊ⒟ߢᳪᨴ߇ᐢ߇ࠆߎ

broth ߦធ⒳ߒ͠ߢᤨ㑆ህ᳇⊛ߦၭ㙃ߒߚ㧚DHL ߅ߣ߇⍮ࠄࠇߡ޿ࠆ㧚ᒰᐕᐲߩ⺞ᩏߢ㧘ࠨ࡞ࡕࡀ࡜ߦߟ޿

ࠃ߮ࠢࡠࡕࠕࠟ࡯ OTAM ߢᬌ಴ߒ㧘ᔅⷐߦᔕߓߡ↢ߡߪ㧘ࡒࡦ࠴⡺߆ࠄઙᬌ಴ߐࠇߚ߇㧘⣺▤಴ⴊᕈᄢ⣺ൻቇᕈ⁁ߩ⏕⹺ࠍⴕߞߚ㧚߹ߚ㧘ᚑಽⷙᩰၮḰߪ㘩ຠⴡ⩶ Oߪᬌ಴ߐࠇߥ߆ߞߚ㧚ࠨ࡞ࡕࡀ࡜ߩᬌ಴₸߽Ყ

↢ᴺ㧔㘩ຠ㧘ᷝട‛╬ߩⷙᩰၮḰ㧕ߦḰ᜚ߒߡⴕߞߚ㧚セ⊛ૐߊ㧘ㆡಾߥ▤ℂ߇ߥߐࠇߡ޿ࠆߣ⠨߃ࠄࠇࠆ㧚↢㘩↪ࠞࠠ߆ࠄ⿒∯⩶ߪᬌ಴ߐࠇߥ߆ߞߚ߇㧘ᚑಽⷙ㧟ᩰၮḰߩ৻⥸⚦⩶ᢙߢၮḰࠍ⿥߃ࠆ߽ߩ߇޽ߞߚ㧚៝౉ᬌᩏ⚿ᨐᬌᩏ⚿ᨐࠍ⴫ߦ␜ߔ㧚ᄢ⣺⩶ߪᬌ૕ਛઙ㧔%㧕߆ࠄᬌ಴ߐࠇߚ㧚ࠨ࡞ࡕࡀ࡜ߪ㧘ࡒࡦ࠴⡺߆ࠄઙߐࠇߚࠞࠠߪ߻߈りߢ޽ߞߚߚ߼㧘ടᎿᲑ㓏ߢᳪᨴ߇޽
ߞߚߣ⠨߃ࠄࠇࠆ㧚㧔ⴊᷡဳ೎ਇ⢻㧕ᬌ಴ߐࠇߚ㧚⣺▤಴ⴊᕈᄢ⣺⩶ Oߪ㧘޿ߕࠇߩᬌ૕߆ࠄ߽ᬌ಴ߐࠇߥ߆ߞߚ㧚߹ߚ㧘޿ߕ㧡߹ߣ߼ࠇߩ↢㘩↪ࠞࠠ߆ࠄ߽⣺Ἳࡆࡉ࡝ࠝ߅ࠃ߮⿒∯⩶ߪᬌ಴੹࿁ߩ⺞ᩏߢ㧘⃻࿷ᵹㅢߒߡ޿ࠆ㘩ຠਛߩ㘩ਛᲥ⚦⩶ߐࠇߥ߆ߞߚ߇㧘หᤨߦታᣉߒߚᚑಽⷙᩰߦ߅޿ߡ㧘ߩᳪᨴ⁁ᴫࠍᛠីߔࠆߎߣ߇ߢ߈ߚ 㧚ߎࠇࠄߩ࠺࡯࠲ߪ㧘ઙߩߺ৻⥸⚦⩶ᢙ߇/g ߣၮḰ㧔/g એਅ㧕੹ᓟ㧘㘩ਛᲥ੍㒐ኻ╷߅ࠃ߮㘩ຠߩ቟ోᕈ⏕଻ߩߚ߼ߦ

ࠍ⿥߃ࠆ߽ߩ߇޽ߞߚ㧚೑↪ߢ߈㧘㘩ຠⴡ↢ⴕ᡽ߦᓎ┙ߟ߽ߩߣ⠨߃ࠄࠇࠆ㧚

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⠨ኤ
㘩ຠߏߣߩᄢ⣺⩶ߩᬌ಴₸ࠍᲧセߔࠆߣ㧘ࡒࡦ࠴⡺߇ෘ↢⋭⋙ୃ㧦㘩ຠⴡ↢ᬌᩏᜰ㊎࡮ᓸ↢‛✬㧘-㧘᧲੩㧘ᣣᧄ౏ⴐⴡ↢දળ㧘‐⡺߇%ߢ޽ࠅ㧘♮ଢᳪᨴߩᜰᮡ⩶ߣߒߡᬌᩏࠍⴕ
߁ᄢ⣺⩶߇ᄙߊߩᬌ૕߆ࠄᬌ಴ߐࠇߡ޿ࠆ㧚㊁⩿߆ࠄᬌ㧘-㧘᧲੩㧘ᣂᣣᧄᴺⷙ㧘

%ߣᦨ߽㜞ߊ㧘ࠬ࠹࡯ࠠ↪‐⡺߇%㧘↢㘩↪㚍⡺߇%㧘↢㊁⩿߅ࠃ߮↢㘩↪ࠞࠠ߇%㧘↢㘩↪

ෘ↢⋭⋙ୃ㧦㘩ຠⴡ↢ዊ౐ᴺ㧘ᐔᚑᐕᐲ

⑔ጟ⋵଻ஜ⑔␩ㇱ㧦ᬌᩏ⺖ᓸ↢‛ࡑ࠾ࡘࠕ࡞㧘-㧘⑔ጟ㧘

಴ߐࠇߚᄢ⣺⩶ߪ㧘࿯ფ߆ࠄ㧘㘩⡺߅ࠃ߮ࡒࡦ࠴⡺߆ࠄᬌ಴ߐࠇߚᄢ⣺⩶ߪ㧘㘩⡺ಣℂߩㆊ⒟ߢᳪᨴߐࠇߚߣផ
⴫㘩ຠߩ㘩ਛᲥ⩶ᳪᨴታᘒ⺞ᩏߦ߅޿ߡᬌ಴ߐࠇߚᬌ૕ᢙ

ᬌᩏᬌ૕ᢙ

ᄢ⣺⩶

ࠨ࡞ࡕࡀ࡜

⣺▤಴ⴊᕈᄢ⣺⩶

⿒∯⩶

O
㊁⩿
ࠬ࠹࡯ࠠ↪
ࡒࡦ࠴⡺

‐⡺









ᬌᩏߖߕ









ᬌᩏߖߕ





㧔ⴊᷡဳ೎ਇ⢻㧕



ᬌᩏߖߕ

↢㘩↪㢚⡺









ᬌᩏߖߕ

↢㘩↪‐⡺





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ᬌᩏߖߕ

↢㘩↪㚍⡺





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ᬌᩏߖߕ

↢㘩↪ࠞࠠ





ᬌᩏߖߕ

ᬌᩏߖߕ



⸘







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

 

⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ╙ภ㧘㧙㧘⾗ᢱᐔᚑ㪈㪍ᐕᐲᗵᨴ∝⚦⩶ᬌᩏ᭎ⷐ
᧛਄శ৻㧘ၳᎹ๺⟤㧘㊁↰ᄙ⟤ᨑ㧘ựፒశብ㧘┻ਛ㊀ᐘ㧘⍹㤤㕏ዏᐔᚑᐕᐲߦᒰ⺖ߢታᣉߒߚᗵᨴ∝⚦⩶ᬌᩏ㗄⋡ߪ㧘࠰ࡦࡀ⿒∯⩶ࠦ࡝ࠪࡦဳ೎㧘ࡃࡦࠦࡑࠗࠪࡦ
⠴ᕈ⣺⃿⩶ߩဳ೎ᬌᩏ㧘ࠦ࡟࡜⩶ᬌᩏߩⴊᷡဳ೎ᬌᩏਗ߮ߦ⣺▤಴ⴊᕈᄢ⣺⩶ߩ࿖┙ᗵᨴ∝⎇ⓥᚲ߳
ߩㅍઃߢ޽ߞߚ㧚ᐔᚑᐕᐲߩ․⸥੐㗄ߪ㧘ࡃࡦࠦࡑࠗࠪࡦ⠴ᕈ⣺⃿⩶ᗵᨴ∝ߦ㑐ߔࠆᬌᩏߢ޽ߞߚ㧚ߎࠇࠄߩ⍮⷗ࠍ฽߼ᒰᐕᐲߩᬌᩏ᭎ⷐࠍႎ๔ߔࠆ㧚㨇ࠠ࡯ࡢ࡯࠼㧦⿒∯⩶㧘ࠦ࡝ࠪࡦဳ೎㧘ࠦ࡟࡜⩶㧘ࡃࡦࠦࡑࠗࠪࡦ⠴ᕈ⣺⃿⩶㧘DNA ⸃ᨆ㧘⣺▤಴ⴊ
ᕈᄢ⣺⩶㧘
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⚦⩶ᬌᩏ㧔⣺▤಴ⴊᕈᄢ⣺⩶ࠍ㒰ߊ㧕ߒ㧘ⴊᷡဳߪ Oߢ޽ߞߚ㧚ᒰᐕᐲߪ㧘⿒∯㧘ࡃࡦࠦࡑࠗࠪࡦ⠴ᕈ⣺⃿⩶㧘ࠦ࡟࡜෸߮Ქ⚛ේᕈᄢ⣺⩶ߩ੐଀㧘ᬌ૕ߦߟ޿ߡᬌᩏࠍታ

㧞ᣉߒߚ㧔⴫㧕㧚⣺▤಴ⴊᕈᄢ⣺⩶ᬌᩏᒰ⎇ⓥᚲߦ៝౉ߐࠇߚ⣺▤಴ⴊᕈᄢ⣺⩶ߪ㧘 O߇㧝࡮㧝 ࠰ࡦࡀ⿒∯⩶ࠦ࡝ࠪࡦဳ೎ᬌᩏ෸߮⿒∯⩶ဳ೎ᬌᩏ

ᩣ㧘 O߇ᩣ㧘 O߇ᩣ㧘 O߇ᩣ㧘 O߇ᩣ

࠰ࡦࡀ⿒∯੐଀㧘ᬌ૕ߦߟ޿ߡࠦ࡝ࠪࡦဳ೎ࠍታᣉ෸߮ OUT:H-߇ ᩣߩ⸘ᩣߢ޽ߞߚ ⴫ 㧕㧚߹ߚ㧘៝ߒߚ㧔⴫㧕㧚ࠦ࡝ࠪࡦဳ೎ߪ㧘ᓸ↢‛ᬌᩏᔅ៤⚦⩶࡮

౉ߐࠇߚ⩶ᩣߪ 㧘⻉ᕈ⁁෸߮ࡌࡠᲥ⚛ߩ⏕⹺ࠍⴕߥߞߚ㧚㧝㧕ߦḰ᜚ߒߚ 㧚ߘߩ⚿ᨐਛ࿖ࠍᣏⴕߒߚ

ߎߩ⚿ᨐ㧘Oߩᩣ෸߮ OߩᩣߪࡌࡠᲥ⚛㒶ᕈߢ޽ᖚ⠪↱᧪ᩣߪࠦ࡝ࠪࡦဳဳ㧘ᩣߪဳ㧘ࡔࠠࠪࠦ෸߮

ߞߚ㧚ᩣߩ Oߩ߁ߜ㧘ᩣ % ߇ Hߢߎߩ߁

ࡈࠖ࡝ࡇࡦᷰ⥶ᱧߩ޽ࠆᖚ⠪↱᧪ᩣߪဳߢ޽ߞߚ㧚ߜᩣ߇ࡌࡠᲥ⚛㧔VT න⁛↥↢ᩣ㧘ᩣ߇ VT෸߮㧝࡮㧞↥↢ᩣ 㧘ᩣ߇ VTන⁛↥↢ᩣ㧘ᩣ㧔%㧕߇ O:H-⌀⩶ᬌᩏ╙

ࡃࡦࠦࡑࠗࠪࡦ⠴ᕈ⣺⃿⩶ᬌᩏH ᐕ᦬෸߮᦬ 㧘ࡃࡦࠦࡑࠗࠪࡦ⠴ᕈ⣺⃿⩶㧔VRE㧕

ᩣߢߔߴߡ VT෸߮↥↢ᩣߢ޽ߞߚ㧚ᩣߩ Oߪ㧘߇ᬌ಴ߐࠇߚ㧚ᒰᚲߢߪ៝౉ߐࠇߚ⩶ᩣ߇㧘⌀ߦ VRE

ߔߴߡ H ⴊᷡဳߪߢ VT↥↢ᩣߢ޽ߞߚ㧚ᩣߩ O

ߢ޽ࠆ߆ุ߆㧘 VRE ߢ޽ࠇ߫ߤߩဳ߆㧘ߘߒߡಽሶ∉ߪ㧘H-ߢ VT↥↢ᩣߢ޽ߞߚ㧚O ⟲ⴊᷡဳ೎ਇ⢻ߣߒቇ⊛ߦ㧘ฦᖚ⠪↱᧪ߩᩣ߇㑐ㅪ߇޽ࠆ߆ุ߆ᬌᩏߒߚ㧚ߡ៝౉ߐࠇߚᩣߩⴊᷡဳ೎ࠍⴕߞߚ⚿ᨐ㧘 O㧦 Hߘߩ⚿ᨐ 㧘៝౉ߐࠇߚฬߩᖚ⠪↱᧪ߩᩣߪ 㧘޿ߕࠇ߽㧘

㧔VT㧕෸߮ O:H㧔VT㧕ߢ޽ߞߚ㧚VT ↥↢ᩣvan B ㆮવሶࠍ᦭ߔࠆ van B ဳ VRE ߣหቯߐࠇߚ㧚߹ᩣߪ 㧘PFGE ߦࠃࠆ DNA ⸃ᨆߩߚ߼ᗵᨴ⎇ߦㅍઃߒߚ㧚ߚ㧘ࡄ࡞ࠬࡈࠖ࡯࡞࠼㨯ࠥ࡞㔚᳇ᵒേࠍ↪޿ߚಽሶ∉ቇᗵᨴ⎇ߢߩ DNA ⸃ᨆߩ⚿ᨐࠍ⴫ߦ␜ߔ㧚ᒰ⎇ⓥᚲ䈪㪛ᬌᩏߦ߅޿ߡ߽㧘ห৻ߩࡄ࠲࡯ࡦࠍ␜ߒߚߎߣ߆ࠄ㧘੕㪥㪘⸃ᨆ䉕ⴕ䈭䈦䈢੐଀䈲䋬὾䈐⡺㑐ㅪ䈪⊒↢䈚䈢㪦㪈㪌㪎੐଀޿ߦㄭ✼ߩᩣߢ޽ࠆߎߣ߇᣿ࠄ߆ߣߥߞߚ㧚㪈ઙ䋨㪍ᩣ䋩䈪䈅䈦䈢䋮㪛㪥㪘⸃ᨆ䈱⚿ᨐ䋬ห৻䊌䉺䊷䊮䉕␜䈚䋮㧝࡮㧠ห৻䈱↱᧪䈮䉋䉎ᗵᨴ䈪䈅䉎䈖䈫䈏್᣿䈚䈢䋮৻ᣇ䋬ᗵᨴ∝

ࠦ࡟࡜⩶ᬌᩏ

ᒰᐕ᦬ቬ௝଻ஜ⑔␩ⅣႺ੐ോᚲ▤ౝߢࡈࠖ࡝ࡇࡦ߆

⎇ⓥᚲ䈪ታᣉ䈚䈢㪛㪥㪘⸃ᨆ䈱⚿ᨐ䈎䉌䋬ኅᣖ䈪὾䈐⡺䉕៨ࠄߩᏫ࿖⠪ߦࠦ࡟࡜ᖚ⠪߇⊒↢ߒ㧘៝౉ߐࠇߚ⩶ᩣߦߟ㘩䈚ᗵᨴ䈚䈢䈫⠨䈋䉌䉏䉎㪍ኅᣖ䋬䉨䊞䊮䊒႐䈪὾䈐⡺䉕䈚䈢㪉޿ߡࠦ࡟࡜⩶ߩ⏕⹺෸߮㑐ㅪᬌᩏࠍታᣉߒߚ㧚ᬌᩏᣇᴺ

੐଀㪋ኅᣖ䋬⸤ఽᚲ෸䈶଻⢒ᚲ䈪䈱㓸࿅ᗵᨴ੐଀䈲䋬䈇䈝ߪᓸ↢‛ᬌᩏᔅ៤

䉏䉅ห৻㪛㪥㪘䊌䉺䊷䊮䉕␜䈜䈖䈫䈏ಽ䈎䈦䈢䋮⚦⩶࡮⌀⩶ᬌᩏ╙ߦḰ᜚ߒߚ 㧚㧝㧕៝౉ߐࠇߚࠦ࡟࡜⩶ߪࠦ࡟࡜Ქ⚛↥↢ᕈㆮવሶࠍ଻᦭
⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲ

㧔‫ޥ‬- ⑔ጟ⋵ᄥቿᐭᏒᄢሼะ૒㊁㧕

 

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ෘ↢⋭⋙ୃ㧘⽷࿅ᴺੱᣣᧄ౏ⴐⴡ↢දળ㧦ᓸ↢‛ᬌᩏᔅ៤⚦⩶࡮⌀⩶ᬌᩏ╙㧧㧚⴫ ᐔᚑᐕᐲᗵᨴ∝⚦⩶ᬌᩏ

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∛ේ⩶
ᬌ૕ᢙ
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䉸䊮䊈⿒∯⩶㪈㪏 ╳⚡㪈㪍㪅㪈㪈㪅㪈㪏 䉸䊮䊈⿒∯⩶㪈㪐 ㆙⾐㪈㪎㪅㪊㪅㪈㪌
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䉸䊮䊈⿒∯⩶
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M 1

2

3

4

5

6

஻⠨
ಽሶ∉ቇᬌᩏ䉕ታᣉ
䉮䊧䊤⩶ᩣ䋱䋬ଢ㪈䇮䊐䉞䊥䊏䊮䈻ᣏⴕಽሶ∉ቇᬌᩏ䉕ታᣉ䉮䊥䉲䊮ဳ೎ਇ⢻䇮䊜䉨䉲䉮䇮䊌䊅䊙䈻ᣏⴕ
䉮䊧䊤⩶䈪䈲䈭䈎䈦䈢㒶ᕈ䉮䊥䉲䊮㪇ဳ䇮ਛ࿖䈻ᣏⴕ䉮䊥䉲䊮㪍ဳ䇮ਛ࿖䈻ᣏⴕ䉮䊥䉲䊮ဳ೎ਇ⢻䇮䊐䉞䊥䊏䊮䈻ᣏⴕ䉮䊥䉲䊮㪍ဳ䇮䉟䊮䊄䊈䉲䉝㩷䊋䊥ፉ䈻ᣏⴕ

R M
M: ࡑ࡯ࠞ࡯
1 : ᖚ⠪ Aᬌ૕⇟ภ 54

2 : ᖚ⠪ Aᬌ૕⇟ภ 104

3 : ᖚ⠪ Bᬌ૕⇟ภ 106

4 : ᖚ⠪ Cᬌ૕⇟ภ 138

5 : ᖚ⠪ Dᬌ૕⇟ภ 143

6 : ᖚ⠪ Eᬌ૕⇟ภ 130

R : ᮡḰ⩶ᩣ Enterococcus faecalis V583

࿑ ᐔᚑᐕ᦬

VRE ಽ㔌⩶ᩣߩࡄ࡞ࠬࡈࠖ࡯࡞࠼࡮ࠥ࡞㔚᳇ᵒേᴺߦࠃࠆㆮવሶဳ೎⚿ᨐ㧚ࡄ࡞ࠬࡈࠖ࡯࡞࠼࡮ࠥ࡞㔚᳇ᵒേߦࠃࠆㆮવሶဳ೎ᬌᩏߢߪ㧘ᬌᩏߒߚ⩶ᩣߪ㧘ᮡḰ⩶ᩣ Enterococcus
faecalis V583 ߣߪ⇣ߥࠆࡄ࠲࡯ࡦࠍ␜ߒߚ㧚ട߃ߡ㧘ߘࠇࠄߩ៝౉ߐࠇߚᩣߪ㧘੕޿ߦห৻ߩᵒേࡄ࠲࡯ࡦࠍ␜ߒߚ㧚ࠃߞߡ㧘⩶ᩣߪ㧘੕޿ߦห৻㧘߹ߚߪߘࠇߦㄭ޿⿠Ḯࠍ᦭ߔࠆ⩶ᩣߢ޽ࠆߣ⠨߃ࠄ
ࠇߚ㧚

 

⴫ ᐔᚑᐕᐲߦ៝౉ߐࠇߚ⣺▤಴ⴊᕈᄢ⣺⩶ߩ2()'⸃ᨆ⚿ᨐ⩶ᩣ⇟ภ ៝౉੐ോᚲ
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☺ደ
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㆙⾐

⊒∝
ᐕ᦬ᣣ
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㪟㪈㪍㪅㪎㪅㪊㪈
㪟㪈㪍㪅㪏㪅㪈㪉
㪟㪈㪍㪅㪌㪅㪉㪊
㪟㪈㪍㪅㪏㪅㪐
㪟㪈㪍㪅㪎㪅㪉
㪟㪈㪍㪅㪏㪅㪈㪋
㪟㪈㪍㪅㪏㪅㪉㪉
㪟㪈㪍㪅㪏㪅㪉㪈
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
㪟㪈㪍㪅㪏㪅㪉㪏
㪟㪈㪍㪅㪏㪅㪉㪉
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩

ዯ಴
ᐕ᦬ᣣ
㪟㪈㪍㪅㪊㪅㪊㪇
㪟㪈㪍㪅㪌㪅㪈㪋
㪟㪈㪍㪅㪌㪅㪉㪍
㪟㪈㪍㪅㪌㪅㪉㪐
㪟㪈㪍㪅㪌㪅㪉㪐
㪟㪈㪍㪅㪌㪅㪉㪐
㪟㪈㪍㪅㪌㪅㪉㪐
㪟㪈㪍㪅㪌㪅㪉㪐
㪟㪈㪍㪅㪍㪅㪈㪇
㪟㪈㪍㪅㪍㪅㪈㪈
㪟㪈㪍㪅㪍㪅㪈㪌
㪟㪈㪍㪅㪍㪅㪈㪎
㪟㪈㪍㪅㪍㪅㪉㪈
㪟㪈㪍㪅㪍㪅㪉㪌
㪟㪈㪍㪅㪍㪅㪉㪐
㪟㪈㪍㪅㪍㪅㪉㪐
㪟㪈㪍㪅㪎㪅㪌
㪟㪈㪍㪅㪎㪅㪌
㪟㪈㪍㪅㪍㪅㪊㪇
㪟㪈㪍㪅㪎㪅㪈㪎
㪟㪈㪍㪅㪎㪅㪈㪎
㪟㪈㪍㪅㪎㪅㪈㪌
㪟㪈㪍㪅㪏㪅㪋
㪟㪈㪍㪅㪏㪅㪋
㪟㪈㪍㪅㪏㪅㪎
㪟㪈㪍㪅㪏㪅㪐
㪟㪈㪍㪅㪏㪅㪈㪇
㪟㪈㪍㪅㪏㪅㪈㪍
㪟㪈㪍㪅㪏㪅㪈㪊
㪟㪈㪍㪅㪏㪅㪐
㪟㪈㪍㪅㪏㪅㪐
㪟㪈㪍㪅㪏㪅㪐
㪟㪈㪍㪅㪏㪅㪐
㪟㪈㪍㪅㪏㪅㪎
㪟㪈㪍㪅㪎㪅㪉㪍
㪟㪈㪍㪅㪎㪅㪉㪎
㪟㪈㪍㪅㪎㪅㪉㪏
㪟㪈㪍㪅㪎㪅㪉㪐
㪟㪈㪍㪅㪏㪅㪉
㪟㪈㪍㪅㪏㪅㪎
㪟㪈㪍㪅㪏㪅㪉
㪟㪈㪍㪅㪏㪅㪈㪎
㪟㪈㪍㪅㪌㪅㪉㪎
㪟㪈㪍㪅㪏㪅㪈㪍
㪟㪈㪍㪅㪎㪅㪍
㪟㪈㪍㪅㪏㪅㪈㪐
㪟㪈㪍㪅㪏㪅㪉㪍
㪟㪈㪍㪅㪏㪅㪉㪍
㪟㪈㪍㪅㪏㪅㪊㪇
㪟㪈㪍㪅㪏㪅㪊㪇
㪟㪈㪍㪅㪏㪅㪊㪇
㪟㪈㪍㪅㪏㪅㪊㪇
㪟㪈㪍㪅㪏㪅㪉㪎
㪟㪈㪍㪅㪐㪅㪍
㪟㪈㪍㪅㪐㪅㪍

ⴊᷡဳ㪭㪫ဳ㪦㪈㪌㪎㪑㪟㪎
㪦㪉㪍㪑㪟㪈㪈
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪐㪈㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪈㪈
㪦㪈㪈㪈
㪦㪈㪈㪈
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎

㪉
㪈
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪉
㪉
㪈㪂㪉
㪉
㪈
㪉
㪉
㪉
㪈
㪈
㪈
㪉
㪉
㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪉
㪈㪂㪉
㪈㪂㪉
㪉
㪈㪂㪉
㪈
㪉
㪉
㪉
㪉
㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
㪈㪂㪉

 

㪛㪥㪘䊌䉺䊷䊮
䋼㪈㪇㪇㪹㫇

㪈㪇㪇䋭㪉㪇㪇㪹㫇㪕㪉㪇㪇㪹㫇

㸉㫂
㪥㪛
㸈㪸
㸈㪸
㸈㪸
㸈㪸
㸈㪸
㸈㪸
㸈㪸
㸉㪹
㸉㪹
㸈㪸
㸉㪹
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㸈㪸
㸈㪸
㸈㪸
㸈㪸
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㸈㪸
㸈㪸
㪥㪛
㪥㪛
㸉㪸
㪥㪛
㸉㪹
㪥㪛
㪥㪛
㪥㪛
㸈㪺
㪥㪛
㪥㪛
㪥㪛
㸈㪺
㪥㪛
㸈㪸
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㸈㪸
㪥㪛
㪥㪛

㪥㪛
㪥㪛
㸈㪸
㸈㪸
㸈㪸
㸈㪸
㸈㪸
㸈㪸
㸈㪸
㪥㪛
㪥㪛
㸈㪸
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㸊
㸊
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㸈㪸
㸈㪸
㸈㪺
㸈㪹
㪥㪛
㸈㪸
㸈㪺
㸈㪹
㸈㪹
㪥㪛
㸈㪸
㸋㩾
㸋㩾
㸋㩾
㸋㩾
㸋㩾
㪥㪛
㸋㩾
㸋㩾

㪥㪛
㪥㪛
㸇
㸇
㸇
㸇
㸇
㸇
㸇
㸉
㪥㪛
㸇
㸉
㪥㪛
㸉
㸉
㸉
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㸉
㸇
㸇
㸇
㸇
㸉
㪥㪛
㪥㪛
㸉
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㸉
㪥㪛
㪥㪛
㸇
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㪥㪛
㸉
㸉
㸉
㸉
㸉
㪥㪛
㸉
㸉

㪧㪝㪞㪜
஻⠨
䉺䉟䊒ฬ
㪉㪊㪇
㪊㪏
㪉㪊㪏
㪉㪊㪏 㪇㪋㪜㪇㪇㪊䈫ห䈛㪉㪊㪏 㪇㪋㪜㪇㪇㪊䈫ห䈛㪉㪊㪏 㪇㪋㪜㪇㪇㪊䈫ห䈛㪉㪊㪏 㪇㪋㪜㪇㪇㪊䈫ห䈛㪉㪊㪏 㪇㪋㪜㪇㪇㪊䈫ห䈛㪉㪊㪐 㪇㪋㪜㪇㪇㪊䈫䈲⇣䈭䉎㪉㪌㪈㪉㪉㪐㪉㪊㪐 㪇㪋㪜㪇㪇㪐䈫ห䈛㪉㪌㪈 㪇㪋㪜㪇㪈㪇䈫ห䈛
㪉㪋㪐
㪉㪋㪐
㪉㪋㪐

㪋㪐㪏
㪋㪐㪏
㪌㪈㪐
㪌㪇㪐
㪌㪇㪐
㪌㪇㪐
㪌㪇㪐
㪌㪉㪊
㪌㪋㪋
㪌㪋㪊
㪌㪉㪊
㪌㪋㪉
㪌㪋㪉
㪌㪉㪏
㪌㪉㪏
㪌㪊㪈
㪌㪉㪋
㪌㪊㪇
㪉㪐㪉
㪌㪊㪋
㪌㪊㪏
㪌㪊㪎
㪊㪇㪎
㪉㪐㪉
㪌㪋㪈
㪌㪊㪊
㪉㪍㪐
㪌㪊㪐
㪉㪐㪉
㪋㪏㪌
㪋㪏㪌
㪋㪏㪌
㪌㪉㪎
㪋㪏㪌
㪌㪋㪇
㪋㪏㪌
㪋㪏㪌

㪇㪋㪜㪇㪈㪌䈫ห䈛㪇㪋㪜㪇㪈㪌䈫ห䈛㪇㪋㪜㪇㪉㪇䈫ห䈛㪇㪋㪜㪇㪉㪇䈫ห䈛㪇㪋㪜㪇㪉㪋䈫ห䈛㪇㪋㪜㪇㪉㪎䈫ห䈛㪇㪋㪜㪇㪉㪎䈫ห䈛㪇㪋㪜㪇㪉㪎䈫ห䈛㪇㪋㪜㪇㪊㪉䈫㪈䊋䊮䊄㆑䈇㪇㪋㪜㪇㪊㪈䈫ห䈛㪇㪋㪜㪇㪊㪌䈫ห䈛㪇㪋㪜㪇㪊㪎䈫ห䈛㪇㪋㪜㪇㪊㪈䈫㪈䊋䊮䊄㆑䈇㪇㪋㪜㪇㪋㪉䈫ห䈛㪇㪋㪜㪇㪋㪉䈫ห䈛㪇㪋㪜㪇㪌㪊䈫ห䈛㪇㪋㪜㪇㪌㪊䈫ห䈛㪇㪋㪜㪇㪌㪊䈫㪈䊋䊮䊄㆑䈇㪇㪋㪜㪇㪌㪊䈫ห䈛㪇㪋㪜㪇㪌㪊䈫ห䈛㪇㪋㪜㪇㪌㪊䈫ห䈛⴫ ᐔᚑᐕᐲߦ៝౉ߐࠇߚ⣺▤಴ⴊᕈᄢ⣺⩶ߩ2()'⸃ᨆ⚿ᨐ⩶ᩣ⇟ภ ៝౉੐ോᚲ
㪇㪋㪜㪇㪍㪈
㪇㪋㪜㪇㪍㪉
㪇㪋㪜㪇㪍㪊
㪇㪋㪜㪇㪍㪋
㪇㪋㪜㪇㪍㪌
㪇㪋㪜㪇㪍㪍
㪇㪋㪜㪇㪍㪎
㪇㪋㪜㪇㪍㪏
㪇㪋㪜㪇㪍㪐
㪇㪋㪜㪇㪎㪇
㪇㪋㪜㪇㪎㪈
㪇㪋㪜㪇㪎㪉
㪇㪋㪜㪇㪎㪊
㪇㪋㪜㪇㪎㪋
㪇㪋㪜㪇㪎㪌
㪇㪋㪜㪇㪎㪍
㪇㪋㪜㪇㪎㪎
㪇㪋㪜㪇㪎㪏
㪇㪋㪜㪇㪎㪐
㪇㪋㪜㪇㪏㪐
㪇㪋㪜㪇㪐㪇
㪇㪋㪜㪇㪐㪈
㪇㪋㪜㪇㪐㪉
㪇㪋㪜㪇㪐㪊
㪇㪋㪜㪇㪐㪋
㪇㪋㪜㪇㪐㪌
㪇㪋㪜㪇㪐㪍
㪇㪋㪜㪇㪐㪎
㪇㪋㪜㪇㪐㪏
㪇㪋㪜㪇㪐㪐
㪇㪋㪜㪈㪇㪇
㪇㪋㪜㪈㪇㪈
㪇㪋㪜㪈㪇㪉
㪇㪋㪜㪈㪇㪊
㪇㪋㪜㪈㪇㪋
㪇㪋㪜㪈㪇㪌
㪇㪋㪜㪈㪇㪍
㪇㪋㪜㪈㪇㪎
㪇㪋㪜㪈㪇㪏
㪇㪋㪜㪈㪇㪐
㪇㪋㪜㪈㪈㪇
㪇㪋㪜㪈㪈㪈
㪇㪋㪜㪈㪈㪉
㪇㪋㪜㪈㪈㪊
㪇㪋㪜㪈㪈㪋
㪇㪋㪜㪈㪈㪌
㪇㪋㪜㪈㪈㪍
㪇㪋㪜㪈㪈㪎
㪇㪋㪜㪈㪈㪏
㪇㪋㪜㪈㪈㪐

㆙⾐
㆙⾐
㆙⾐
㆙⾐
㆙⾐
㆙⾐
㆙⾐
㆙⾐
☺ደ
☺ደ
☺ደ
ਭ⇐☨
ਭ⇐☨
ਭ⇐☨
ਭ⇐☨
ਭ⇐☨
╳⚡
☺ደ
♻ፉ
╳⚡
╳⚡
౎ᅚ
౎ᅚ
౎ᅚ
╳⚡
ਭ⇐☨
ਭ⇐☨
㕷ᚻ
㕷ᚻ
㕷ᚻ
㕷ᚻ
㕷ᚻ
㕷ᚻ
㕷ᚻ
㕷ᚻ
㕷ᚻ
㕷ᚻ
㕷ᚻ
੩▽
㕷ᚻ
☺ደ
ਭ⇐☨
㆙⾐
ਭ⇐☨
☺ደ
☺ደ
ਭ⇐☨
ਭ⇐☨
ਭ⇐☨
ਭ⇐☨

⊒∝
ᐕ᦬ᣣ
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
㪟㪈㪍㪅㪏㪅㪈㪐
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
㪟㪈㪍㪅㪏㪅㪉㪎
㪟㪈㪍㪅㪐㪅㪊
㪟㪈㪍㪅㪏㪅㪉㪈
㪟㪈㪍㪅㪐㪅㪈㪈
㪟㪈㪍㪅㪐㪅㪈㪈
㪟㪈㪍㪅㪏㪅㪊㪇
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㪟㪈㪍㪅㪐㪅㪈㪏
㪟㪈㪍㪅㪏㪅㪉㪎
䋨଻⩶⠪䋩
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䋨଻⩶⠪䋩
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䋨଻⩶⠪䋩㪟㪈㪎㪅㪈㪅㪈㪐
䋨଻⩶⠪䋩
䋨଻⩶⠪䋩
㪟㪈㪎㪅㪉㪅㪉㪈

ዯ಴ᐕ᦬ᣣ㪟㪈㪍㪅㪐㪅㪍
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㪟㪈㪍㪅㪈㪇㪅㪈㪇㪟㪈㪍㪅㪈㪇㪅㪈㪈㪟㪈㪍㪅㪈㪇㪅㪈㪈㪟㪈㪍㪅㪈㪇㪅㪐
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㪟㪈㪎㪅㪈㪅㪉㪍
㪟㪈㪎㪅㪉㪅㪉
㪟㪈㪎㪅㪉㪅㪉
㪟㪈㪎㪅㪉㪅㪉㪌

ⴊᷡဳ㪭㪫ဳ㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
㪦㪈㪌㪎㪑㪟㪎
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㪦㪈㪌㪎㪑㪟㪄
㪦㪈㪌㪎㪑㪟㪎

㪈㪂㪉
㪈㪂㪉
㪈㪂㪉
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㪉

㪛㪥㪘䊌䉺䊷䊮
䋼㪈㪇㪇㪹㫇

㪈㪇㪇䋭㪉㪇㪇㪹㫇㪕㪉㪇㪇㪹㫇

㪥㪛
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㪧㪝㪞㪜
஻⠨
䉺䉟䊒ฬ
㪋㪏㪌 㪇㪋㪜㪇㪌㪊䈫ห䈛㪋㪏㪌 㪇㪋㪜㪇㪌㪊䈫ห䈛㪋㪏㪌 㪇㪋㪜㪇㪌㪊䈫ห䈛㪋㪏㪌 㪇㪋㪜㪇㪌㪊䈫ห䈛㪋㪏㪌 㪇㪋㪜㪇㪌㪊䈫ห䈛㪋㪏㪌 㪇㪋㪜㪇㪌㪊䈫ห䈛㪋㪏㪌 㪇㪋㪜㪇㪌㪊䈫ห䈛㪋㪏㪌 㪇㪋㪜㪇㪌㪊䈫ห䈛㪌㪉㪍 㪇㪋㪜㪇㪌㪊䈫㪈䊋䊮䊄㆑䈇㪋㪏㪌 㪇㪋㪜㪇㪌㪊䈫ห䈛㪋㪏㪌 㪇㪋㪜㪇㪌㪊䈫ห䈛㪌㪉㪌㪌㪉㪌 㪇㪋㪜㪇㪎㪉䈫ห䈛㪌㪉㪌 㪇㪋㪜㪇㪎㪉䈫ห䈛㪌㪉㪌 㪇㪋㪜㪇㪎㪉䈫ห䈛㪌㪉㪌 㪇㪋㪜㪇㪎㪉䈫ห䈛
㪏㪊㪋
㪉㪐㪉
㪉㪐㪉
㪉㪍
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㪏㪉㪏
㪸㪈㪇
㪸㪐
㪸㪐
㪸㪐
㪏㪉㪊

㪇㪋㪜㪇㪋㪉䈫ห䈛㪇㪋㪜㪇㪋㪉䈫ห䈛㪇㪋㪜㪇㪐㪈䈫ห䈛㪇㪋㪜㪇㪐㪈䈫ห䈛㪇㪋㪜㪇㪐㪌䈫ห䈛㪇㪋㪜㪇㪐㪎䈫ห䈛㪇㪋㪜㪇㪐㪎䈫ห䈛㪇㪋㪜㪇㪐㪎䈫ห䈛㪇㪋㪜㪇㪐㪎䈫ห䈛㪇㪋㪜㪇㪐㪎䈫ห䈛㪇㪋㪜㪇㪐㪎䈫ห䈛㪇㪋㪜㪇㪐㪎䈫㪈䊋䊮䊄㆑䈇㪇㪋㪜㪇㪐㪎䈫ห䈛㪇㪋㪜㪇㪐㪎䈫㪉䊋䊮䊄㆑䈇㪇㪋㪜㪇㪐㪎䈫ห䈛㪇㪋㪜㪇㪐㪎䈫㪉䊋䊮䊄㆑䈇㪇㪋㪜㪇㪐㪎䈫ห䈛㵱㪌㪎㪎㵱䈫ห䈛㪇㪋㪜㪈㪈㪍㩷䈫ห䈛㪇㪋㪜㪈㪈㪍㩷䈫ห䈛

DNA ߩࡄ࠲࡯ࡦߪ㧘XbaI ߢ㉂⚛ಣℂߒߚ DNA ߩ PFGE ௝ࠍಽሶ㊂ 㧨kb㧘-kb㧘㧪kb ߩㇱಽࠍਅ⸥ߦ␜ߔࠃ߁ߦಽߌ㧘ߐࠄߦࡃࡦ࠼㆑޿ߢߪ࠳࠶ࠪࡘ㧔̉㧕ࠍઃߌ㧘߹ߚߎࠇࠄߦ⹥ᒰߒߥ޿ࡄ࠲࡯ࡦߪ ND ߣ⴫⸥ߐࠇ㧘ߎࠇࠄߩ⚵ߺวࠊߖߦࠃࠅဳ೎ߐ
ࠇߡ޿ࠆ㧚߹ߚ㧘DNA ⸃ᨆ⚿ᨐߩ⴫⸥ᣇᴺߪ㧘ᐕߪࡄ࠲࡯ࡦฬߣ⇟ภߢ⴫ߐࠇ㧘ᐕ߆ࠄߪ⇟ภߩߺߣߥߞߚ㧚㧨kb㧦
I--abc Τ--abcdefghjkmn Υ--abcdefghkmΦ--ab Χ--abc Ψ--⒳ߩߺ Ω--⒳ߩߺ㧘⸘⒳㘃
-kb㧦 Σ--⒳ߩߺ㧘Τ--abcde㧘Υ--ab㧘Φ--⒳ߩߺ㧘Χ--ab㧘Ψ--⒳ߩߺ㧘⸘⒳㘃
㧪kb㧦 Σ--⒳㧘Τ-Ψฦ⒳ߩߺ㧘⸘⒳㘃

 ⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ╙ภ㧘㧙㧘⾗ᢱᐔᚑ㧘ᐕᐲᕈེࠢ࡜ࡒࠫࠕ㧔%JNCO[FKC VTCEJQOCVKU㧕᛫૕ᬌᩏ⚿ᨐߩ᭎ⷐ┻ਛ㊀ᐘ㧘ựፒశብ㧘㊁↰ᄙ⟤ᨑ㧘᧛਄శ৻㧘ၳᎹ๺⟤㧘⍹㤥㕏ዏ⑔ጟ⋵ߢߪ㧘ᐔᚑ 15 ᐕ 3 ᦬ࠃࠅᕈེࠢ࡜ࡒࠫࠕ᛫૕ᬌᩏࠍታᣉߒߡ޿ࠆ㧚ᐔᚑ 17 ᐕ 3 ᦬߹ߢߦᒰ
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382 ฬ㧕ߢ޽ߞߚ㧚ߘߩ߁ߜ㧘ࠢ࡜ࡒࠫࠕ᛫

ߺࠆߣ㧘20 ᱦઍ߇ᦨ߽ᄙߊ㧘↵ᕈߢߪ 20  30 ᱦઍߦ㧘ᅚᕈߢߪ 20 ᱦઍߦᄢ߈ߥࡇ࡯ࠢ߇ߺࠄࠇߚ㧚㨇ࠠ࡯ࡢ࡯࠼㧦ᕈེࠢ࡜ࡒࠫࠕ㧘Chlamydia trachomatis Ig A㧘Ig G㧘ELISA㨉
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ᕈེࠢ࡜ࡒࠫࠕᗵᨴ∝ߪᣣᧄߢᦨ߽ᄙ޿ᕈᗵᨴ∝⹜⮎෸߮ᯏེ

᛫ࠢ࡜ࡒࠫࠕ᛫૕ߩᬌᩏߦߪ㧘ᣣ┙ൻᚑᎿᬺࢃ⵾ߩࠠ

㧔 STD㧕ߢ޽ࠆ㧚ᗵᨴ∝ᴺߢߪ㧘5 㘃ᗵᨴ∝ߣߒߡᕈᗵ

࠶࠻㧘ࡅ࠲ࠩࠗࡓ

ᨴ∝ቯὐ߆ࠄߩႎ๔߇⟵ോߠߌࠄࠇߡ޿ࠆ㧚ૐᐕ㦂ጀߩⓣࡑࠗࠢࡠࡊ࡟࡯࠻㧔࠽࡞ࠫࠚ࠿ࡦࠢ␠⵾ 㧕㧘ࡊ࡟࡯࠻ᅚᕈߦᄙߊ㧘ᚑੱߢߪᕈⴕὑߦࠃߞߡᗵᨴߔࠆ㧚ᦨㄭߢࠪ࡯࡞㧔૑෹ࡌ࡯ࠢ࡜ࠗ࠻␠⵾ 㧕㧘ࠛ࠶ࡍࡦ࠼࡞ࡈࡇࡍ

ߪ㧘ೋ੤ᐕ㦂ߩૐਅߦ઻ߞߡ 10 ઍᅚᕈߩᗵᨴ₸ߩ㜞ߐ࠶࠻߅ࠃ߮࠴࠶ࡊ㧔Eppendorff ␠ ⵾㧕㧘ࡒ࡝㧽᳓㧔Milli Q߇ 㧘዁᧪ߩਇᅧߦ❬߇ࠆߩߢߪߥ޿߆ߣᘷᘦߐࠇߡ޿ࠆ 㧚

SP-UF㧘Millipore ␠ ⵾ࠍ↪޿ߡ 㧘㔚᳇ᛶ᛫ᅚᕈߩ႐ว㧘ᗵᨴߒߡ޿ߡ߽⥄ⷡ∝⁁߇ਲߒ޿ߚ߼⸻ᢿ

એ਄ߩ᳓ࠍ↪޿ߚ 㧚㧕㧘ࡑࠗࠢࡠࡊ࡟࡯࠻↪ᵞᵺེ㧔ࠝ࡯࡮ᴦ≮ߦ⥋ࠄߥ޿႐ว߇ᄙߊ㧘⥄ⷡߩߥ޿߹߹ߦ↵ᕈࡄ࠻ࡒ࠾࠙ࠜ࠶ࠪࡖ࡯

ࠢ࡜ࡒࠫࠕࠍ↪޿ߚ㧘ߘߩઁߦ㧘96

18.3 㧹ǡ࡮cm

AMW-8㧘 BioTec ␠ ⵾ 㧕㧘ࡑࠗࠢ࡯࠻࠽࡯߿಴↥ఽߦᗵᨴߐߖࠆߎߣ߇޽ࠆ㧚ᅧᇚᬌ⸻ߦ

ࡠࡊ࡟࡯࠻࡝࡯࠳࡯㧔 MTP-120㧘 ᣣ┙⵾૞ᚲ⵾ 㧕㧘ࠗࡦ߅޿ߡ㧘ᱜᏱᅧᇚߩ 3  5%ߦࠢ࡜ࡒࠫࠕ଻᦭⠪߇⷗಴ࠠࡘࡌ࡯࠲࡯㧔PCI-300㧘 ੗ౝ⋓ᩕၴ⵾ 㧕㧘ࡊ࡟࡯࠻ࡒࠠ

ߐࠇࠆߎߣ߆ࠄ㧘⥄ⷡ∝⁁ߩߥ޿ᗵᨴ⠪ߪ߆ߥࠅ޽ࠆ߽

ࠨ࡯㧔Monoshake㧘 Labortechnik AG ␠ ⵾㧕߅ࠃ߮ࡒࠠࠨ

ߩߣផ᷹ߐࠇߡ޿ࠆ 㧚࡯㧔Vortex-Genez㧘 Scientific Industries ␠ ⵾㧕ࠍ↪޿ߚ㧚⑔ጟ⋵ߢߪ㧘ᐔᚑ 15 ᐕ 3 ᦬ࠃࠅᕈེࠢ࡜ࡒࠫࠕᗵᨴ

㧞࡮㧠

ᬌᩏᣇᴺ

∝ߦߟ޿ߡ㧘Chlamydia trachomatis㧔 Ct㧕ߩ᛫૕ᬌᩏࠍ

ࠠ࠶࠻ౝߩ⹜⮎ߣࡊ࡟࡯࠻㧘ߥࠄ߮ߦᬌ૕ࠍቶ᷷ߦ߹

⋵ౝ 13 ࡩᚲߩ଻ஜ⑔␩ⅣႺ੐ോᚲߢ㧘HIV ᛫૕ᬌᩏ㧘ߢᚯߒ 㧘Ig A ᛫૕᷹ቯߩ႐วߪᬌ૕ࠍᏗ㉼↪✭ⴣᶧ㧔ᵞ᪢Ქᬌᩏߣ౒ߦ㧘ήᢱߢታᣉߒߡ޿ࠆ㧚ᒰ⎇ⓥᚲߢߪ㧘ᵺᶧߦหߓ㧕ߢ 21 ୚ߦᏗ㉼ߒ㧘Ig G ᛫૕᷹ቯߩ႐วߦ

ߎࠇࠄߩ଻ஜ⑔␩ⅣႺ੐ോᚲ߆ࠄㅳߦ৻ᐲ៝౉ߐࠇࠆᬌ

ߪᬌ૕ࠍᏗ㉼↪✭ⴣᶧߢ 210 ୚ߦᏗ㉼ߒߚ㧚ߘߩᓟ㧘ࡉ૕ߦߟ޿ߡ᛫૕ᬌᩏࠍታᣉߒߡ޿ࠆ㧚ᧄⓂߢߪ 㧘ᐔᚑ 15࡜ࡦࠢ↪ߣߒߡᏗ㉼↪✭ⴣᶧࠍ 1 ࠙ࠚ࡞㧘㒶ᕈኻᾖⴊᷡ

ᐕ 3 ᦬߆ࠄᐔᚑ 17 ᐕ 3 ᦬߹ߢߩᬌᩏ⚿ᨐߩ᭎ⷐߦߟ޿

ࠍ 2 ࠙ࠚ࡞㧘㓁ᕈኻᾖⴊᷡࠍ 2 ࠙ࠚ࡞ߦฦ 100 Ǵ L ߕߡႎ๔ߔࠆ㧚

ߟಽᵈߒ 㧘એ㒠 㧘Ig A ᛫૕᷹ቯ↪Ꮧ㉼ᬌ૕ࠍߘࠇߙࠇ 㧘㧞ᣇᴺ

100 Ǵ L ߕߟಽᵈߒߚ 㧚Ig G ᛫૕᷹ቯߩ႐วߦ߽ห᭽ߦ 㧘㧞࡮㧝 ᬌ૕
ᐔᚑ 15 ᐕ 3 ᦬߆ࠄᐔᚑ 17 ᐕ 3 ᦬ߦ߆ߌߡ 㧘ㅳߦ৻ᐲ 㧘⋵ౝ 13 ࡩᚲߩ଻ஜ⑔␩ⅣႺ੐ോᚲߢណⴊߐࠇ㧘ಽ㔌ߐࠇߚⴊᷡࠍ↪޿ߚ㧚㧞࡮㧞ⴊᷡਛߩ᛫ࠢ࡜ࡒࠫࠕ᛫૕㧔Ig A ߅ࠃ߮ Ig G㧕ߦߟ⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲ

ᷡࠍ 2 ࠙ࠚ࡞㧘㓁ᕈኻᾖⴊᷡࠍ 2 ࠙ࠚ࡞ߦฦ 100 Ǵ Lߕߟಽᵈߒ㧘એ㒠㧘Ig

G ᛫૕᷹ቯ↪Ꮧ㉼ᬌ૕ࠍߘࠇߙ

ࠇ㧘100 Ǵ L ߕߟಽᵈߒߚ㧚ᬌᩏ㗄⋡޿ߡᬌᩏࠍታᣉߒߚ㧚ࡉ࡜ࡦࠢ↪ߣߒߡᏗ㉼↪✭ⴣᶧࠍ 1 ࠙ࠚ࡞㧘㒶ᕈኻᾖⴊᰴߦ㧘ࡊ࡟࡯࠻ࠪ࡯࡞ࠍ⾍ࠅ㧘37 ͠ߢ 60 ಽ㑆ࠗࡦࠠࡘࡌ࡯࠻ߒߚ㧚ߘߩᓟ㧘ࡊ࡟࡯࠻ࠪ࡯࡞ࠍ೸߇ߒ㧘ࡑࠗࠢࡠࡊ࡟࡯࠻↪ᵞᵺེߢᵞᵺᶧࠍฦ࠙ࠚ࡞ 300 Ǵ L ߕ㧔‫ޥ‬- ⑔ጟ⋵ᄥቿᐭᏒᄢሼะ૒㊁㧕 ߟಽᵈߒ㧘 3 ࿁ᵞᵺߒߚ㧚ᰴߦ㧘㉂⚛㧔ࠕ࡞ࠞ࡝ࡈࠜࠬ

⸘▚ᑼࠍ↪޿ߡࠞ࠶࠻ࠝࡈࠗࡦ࠺࠶ࠢࠬࠍ⸘▚ߒ㧘㒶ᕈࡈࠔ࠲࡯࠯㧕ᮡ⼂᛫ࡅ࠻ Ig A ᛫૕ 100 Ǵ L ࠍ Ig A ᛫૕㧔㧙 㧕㧘㓁ᕈ㧔㧗㧕㧘߹ߚߪᡆ㓁ᕈ㧔r㧕ࠍ್ቯߒߚ㧚᷹ቯ↪࠙ࠚ࡞ߦ㧘㉂⚛ᮡ⼂᛫ࡅ࠻ Ig G ᛫૕ 100 Ǵ L ࠍ

㧟

Ig

⚿ᨐ

G ᛫૕᷹ቯ↪࠙ࠚ࡞ߦߘࠇߙࠇട߃㧘ࡊ࡟࡯࠻ࠪ࡯

࡞ࠍ⾍ࠅ 㧘37 ͠ߢ 60 ಽ㑆ࠗࡦࠠࡘࡌ࡯࠻ߒߚ 㧚ߘߩᓟ㧘
ห᭽ߦᵞᵺࠍ 3 ࿁ⴕߞߚ㧚ᦨᓟߦ㧘ฦ࠙ࠚ࡞ߦၮ⾰ᶧ㧔p-࠾࠻ࡠࡈࠚ࠾࡞࡝ࡦ㉄ṁᶧ 㧕ࠍ 100 Ǵ L ߕߟട߃ 㧘ቶ᷷ߢ 10 ಽ㑆෻ᔕߐߖߚ㧚෻ᔕ⚳ੌᓟ㧘⋥ߜߦ஗ᱛᶧ㧔 3N ᳓㉄ൻ࠽࠻࡝࠙ࡓ฽ࠕ
ࠫൻ࠽࠻࡝࠙ࡓ㧕ࠍ 25 Ǵ L ߕߟฦ࠙ࠚ࡞ߦട߃㧘ࡊ࡟࡯࠻ࡒࠠࠨ࡯ߢ 10 ⑽ᷙ๺ߒߚ㧚ߘߩᓟ㧘ࡑࠗࠢࡠࡊ࡟
࡯࠻࡝࡯࠳࡯ࠍ↪޿㧘405 nm ߩๆశᐲࠍ᷹ቯߒߚ㧚᷹

ᐔᚑ 15 ᐕ 3 ᦬߆ࠄᐔᚑ 17 ᐕ 3 ᦬߹ߢߩ Ct ᛫૕ᬌᩏଐ㗬ઙᢙࠍ࿑ 1 ߦ␜ߔ 㧚✚ଐ㗬ઙᢙߪ 872 ઙߢ 㧘ߘߩౝ 㧘↵ᕈߪ 490 ઙ㧘ᅚᕈߪ 382 ઙߢ޽ߞߚ㧚Ct ᛫૕㓁ᕈ⠪ߪ㧘↵ᕈ 77 ฬ㧘ᅚᕈ 112 ฬߢ޽ࠅ㧘ߘߩ㓁ᕈ₸ߪ↵ᕈ
15.7%㧘ᅚᕈ 29.3%ߢ ޽ߞߚ㧚ో૕ߢߪ 23.1%߇㓁ᕈߢ޽ߞߚ㧚 Ct ᛫૕㓁ᕈ⠪ߩᐕ㦂᭴ᚑࠍ⷗ࠆߣ㧘࿑ 2 ߦ␜ߔࠃ߁ߦ㧘20 ઍߦᦨ߽ᄙߊ㓸ਛߒߡ޿ߚ㧚࿑ 3 ߦ↵ᅚ೎ߩ Ct ᛫૕㓁ᕈ⠪ߩᐕ㦂᭴ᚑࠍ␜ߔ㧚↵ᕈߩ႐วߪ 20
ઍ߆ࠄ 30 ઍߦ߆ߌߡࡇ࡯ࠢ߇⷗ࠄࠇ㧘ᅚᕈߩ႐วߪ 20ቯߩ㓙ߩ࡝ࡈࠔ࡟ࡦࠬߦߪ 630 nm ߩๆశᐲࠍ↪޿ߚ㧚ઍߦᄢ߈ߥࡇ࡯ࠢ߇⷗ࠄࠇߚ㧚ߐࠄߦ㧘Ig

᷹ቯ⚿ᨐߪ㧘ࠠ࠶࠻ߦᷝઃߐࠇߚ⺑᣿ᦠߦ⸥タߐࠇߚ G ᛫૕㓁ᕈ

߇␜ߔ Ct ߦᣢᓔᱧߩ޽ࠆ⠪ߪ 165 ฬߢ㧘ో㓁ᕈ⠪ᢙߩߎߣ߇ႎ๔ߐࠇ㧘ో࿖㜞╬ቇᩞ PTA ㅪวળߩࠕࡦࠤ࡯

87.3%ߢ޽ߞߚ㧚࠻⺞ᩏߢ߽㧘 STD ߦኻߔࠆ⍮⼂ߩૐߐ߇᣿ࠄ߆ߦߥߞ

㧠ߡ߅ࠅ  㧘੹ᓟߩቇᩞߢߩᕈᢎ⢒ߩ޽ࠅᣇ߽⠨߃⋥ߐߨ⠨ኤᕈེࠢ࡜ࡒࠫࠕᗵᨴ∝ߪ⃻࿷㧘ᦨ߽᜛ᄢߒߡ޿ࠆ STD߫ߥࠄߥ޿ߛࠈ߁㧚ߢ޽ࠅ㧘․ߦ㧘16  25 ᚽ߹ߢߩ⧯ᐕᅚᕈߦ࿶ୟ⊛ߦᄙ

ᢥ₂ߊ⷗ࠄࠇࠆ㧚ߒ߆߽ᅚᕈߩ႐ว㧘⥄ⷡ∝⁁ߦਲߒ޿ߎߣ

߇㧘዁᧪ߩਇᅧ∝߿ሶችᄖᅧᆼߦ❬߇ࠆෂ㒾ᕈࠍሺࠎߢ޿ࠆ 㧚ෘ↢ഭ௛⋭ߩᗵᨴ∝⊒↢േะ⺞ᩏߦࠃࠆߣ 㧘ᦨㄭ㧘ᐔᚑᐕ᦬ᣣ㧚࠹ࠖ࡯ࡦࠛ࡯ࠫࡖ࡯㧔15  19 ᚽ㧕ߩᕈེࠢ࡜ࡒࠫࠕᗵ
ᨴ∝߳ߩ⟕ᖚ₸߇Ⴧട௑ะߦ޽ࠅ㧘ᖚ⠪ߩ⧯ᐕൻߩ௑ะ

ෘ↢ഭ௛⋭㧦ᗵᨴ∝⊒↢േะ⺞ᩏ㧘╙ㅳภ㧘⑔ጟ⋵⚿ᩭ࡮ᗵᨴ∝⊒↢േะ⺞ᩏᆔຬળ㧦ᐔᚑᐕ
ᐲ⑔ጟ⋵⚿ᩭ࡮ᗵᨴ∝⊒↢േะ⺞ᩏ੐ᬺ⾗ᢱ㓸㧚ᾢᧄᖝ᣿㧦HIV ᗵᨴ∝ߩ∉ቇ⎇ⓥ㧙 STD ∝଀ߦ߅ߌ߇⋉‫ޘ‬㧘㗼⪺ߦߥߞߡ߈ߡ޿ࠆ 㧚ᕈེࠢ࡜ࡒࠫࠕᗵᨴ

ࠆ HIV ෸߮ STD 㑐ㅪ᛫૕ߦ㑐ߔࠆⴊᷡ∉ቇ⊛⺞ᩏ⠪ᩮ⛘ߩߚ߼ߦߪ⧯ᐕ⠪ኻ╷߇㊀ⷐߣߐࠇࠆᚲએߢ޽

㧙㧘ᐔᚑᐕᐲෘ↢⑼ቇ⎇ⓥႎ๔ᦠ㧚





ࠆ㧚
ᐔᚑ 16 ᐕᐲ⑔ጟ⋵⚿ᩭ࡮ᗵᨴ∝⊒↢േะ⺞ᩏ੐ᬺ⾗

Curable Sexually Transmitted Infections Overview andEstimates㧔㧕㧚ᢱ㓸 ߦࠃࠆߣ㧘⑔ጟ⋵ߢ߽ଐὼ㧘56&ߦභ߼ࠆᕈེࠢ࡜
ࡒࠫࠕߩ⟕ᖚ₸ߪ㜞޿㧚ߎߩ࠺࡯࠲ߪቯὐႎ๔߆ࠄ਄߇㧔+I )᛫૕㓁ᕈ⠪㧕ߢ޽ࠆ㧚ᅚᕈߩ႐วߪ㧘⥄ⷡ∝⁁ߦਲߒ޿ߎߣ߽޽ࠅ㧘ή∝୥ᗵᨴ⠪߇㓝ࠇߡ޿ࠆน⢻ᕈ᦬ႎ㧘ᐔᚑᐕ

ᾢᧄᖝ᣿㧦ᕈᗵᨴ∝ߩലᨐ⊛ߥ⊒↢േะ⺞ᩏߦ㑐ߔࠆ⎇ⓥ㧘ᐔᚑ 12 ᐕᐲෘ↢⑼ቇ⎇ⓥႎ๔ᦠ㧚ቯߒߡ޿ࠆߛߌߢ%V᛫ේࠍᬌ಴ߒߡ޿ࠆࠊߌߢߪߥ޿㧚ߒ߆߽㧘 Ct ᛫૕㓁ᕈ⠪ߩᄙߊߪࠢ࡜ࡒࠫࠕ∝ߩᣢᓔ⠪ਃ㡞ᑝ❥ࠄ㧦∛ේᓸ↢‛ᬌ಴ᖱႎ᦬ภ㧚ߞߡ߈ߚ࠺࡯࠲ߢ㧘%Vߩ᛫ේᬌᩏ㓁ᕈᢙ߇᜼ߍࠄࠇߡ޿
ࠆ㧚ᒰ⎇ⓥᚲߢታᣉߒߚᬌᩏߪ㧘%V᛫૕଻᦭ߩ᦭ήࠍ್

WHO㧦 Global Prevalence and Incidence of Selectedෘ↢ഭ௛⋭ࡎ࡯ࡓࡍ࡯ࠫ㧘
http//www.mhlw.go.jp/shingi/2005/03/txt/s0307-1.txt.

߽ᜰ៰ߐࠇߡ޿ࠆ㧚ߐࠄߦ㧘ᜬ⛯ᕈᗵᨴ߽໧㗴ߣߥߞߡ߅ࠅ㧘ᴦ≮߇ਇචಽߥ႐วߪ߽ߜࠈࠎߩߎߣ㧘චಽߥᴦ≮ࠍⴕߞߚߦ߽㑐ࠊࠄߕᜬ⛯ᕈᗵᨴ߇໧㗴ߣߥࠆ∝଀ ߽޽ࠆ㧚ߎࠇࠄߩ໧㗴ὐࠍ〯߹߃ߚᣂߚߥᬌᩏᘒ൓᭴▽߇ᦸ߹ࠇࠆ㧚ᦨㄭߪ㧘ࠝ࡯࡜࡞࠮࠶ࠢࠬ߇ߏߊᣣᏱ⊛ߥᕈⴕേߦߥࠅ㧘ߘߩ⚿ᨐ㧘ࠢ࡜ࡒࠫࠕຜ㗡ᗵᨴ߇Ⴧ߃㧘ᗵᨴ᜛ᄢߩ৻࿃ߣߥߞߡ޿ࠆ  㧚ߒߚ߇ߞߡ㧘ᕈེࠢ࡜ࡒࠫࠕᗵᨴ߇⷗ߟ߆ߞߚ႐ว㧘Ⓧᭂ⊛ߦຜ㗡ᗵᨴ⁁ᴫ߽ᬌᩏߔߴ߈ߢ޽ࠆߣႎ๔ߐࠇߡ޿ࠆ  㧚%Vߩᛩ⮎ᴦ≮ߦߟ޿ߡߪ㧘଀߃߫㧘ࠕࠫࠬࡠࡑࠗࠪࡦ OIන࿁ᛩਈࠍታᣉߒߚ႐ว 㧘ᴦ≹₸ߪሶች㗪▤ߢߪ㧑ߛߞߚ߇ 㧘ຜ㗡ߢߪ㧑ߦㆊ߉ߥ߆ߞߚ㧚ߎࠇߪ㧘ੱߦੱ߇㒰⩶ߦᄬᢌߔࠆ⸘▚ߢ޽ࠆ㧚ߘࠇߛߌ㒰⩶ߒߦߊ޿ࠊߌߢ޽
ࠆ߇㧘ߎߩࠃ߁ߥ∝଀ߢߪ᛫૕ଔߩਅ㒠߇ਇචಽߥ੐߇
ᄙߊ㧘ᵈᗧ߇ᔅⷐߢ޽ࠆ 㧚
STD ߪࠛࠗ࠭࠙ࠖ࡞ࠬ㧔HIV㧕ߩᗵᨴ₸ࠍ 2  4 ୚㜞߼ࠆߣߐࠇߡ޿ࠆ߇ 㧘ᣣᧄߢߪ*+8ᗵᨴ⠪ߣᖚ⠪ߩว⸘߇㧝ਁੱࠍ⓭⎕ߒߚਛ㧘⧯ᐕ⠪ࠍਛᔃߦ῜⊒⊛ߥ*+8ᗵᨴߩჇട߇ ᔨߐࠇߡ޿ࠆ㧚56&߇*+8ߩᗵᨴ₸ࠍ㜞߼ࠆߎߣ߆ࠄ߽㧘⧯ᐕጀ߳ߩ✕ᕆߥ56&ኻ╷ߩᔅⷐᕈࠍᗧ๧
ߒߡ޿ࠆ㧚56&ߩ⟕ᖚ₸ߪ㧘᰷☨ߩᅚሶ㜞↢߇ 1 - 4%ߢ
޽ࠆߩߦኻߒ㧘ᣣᧄߢߪ 13.1 㧑ߢ㧘⟕ᖚ₸ࠍᲧߴߡ߽
ᣣᧄߪ㓙ߛߞߡ㜞޿ 㧚ᦨㄭ 㧘ෘ↢ഭ௛⋭ߩ⺞ᩏ ߦࠃࠅ 㧘ᕈ૕㛎ߩ޽ࠆ㜞ᩞ↢ߩ߁ߜ⚂ 1 ഀ߇ Ct ߦᗵᨴߒߡ޿ߚ ో࿖㜞╬ቇᩞ PTA ㅪวળ㧦㧔␠㧕ో㜞㧼ㅪળႎ 㧘╙ 51ภ㧘ᐔᚑ 17 ᐕ 4 ᦬ 15 ᣣ㧚 

⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ╙ภ㧘㧙㧘⾗ᢱᗵᨴ∝⊒↢േะ⺞ᩏ੐ᬺߦ߅ߌࠆ࠙ࠗ࡞ࠬᬌᩏ⚿ᨐ㧔ᐕ㧕⍹ᯅື਽㧘ᳯ⮮⦟᮸㧘᫃ේᷕ⌬㧘਎⦟ᥰਯ㧘ජ‫ޘ‬๺ൎᏆᗵᨴ∝⊒↢േะ⺞ᩏ੐ᬺߦ߅ߌࠆ∛ේ૕ᖱႎߩ৻Ⅳߣߒߡ㧘2004 ᐕߪ⋵ౝ 16 ࠞᚲߩ∛ේ૕ቯὐක≮ᯏ㑐߆ࠄ 305 ઙߩᬌᩏ᧚ᢱ߇៝౉ߐࠇߚ㧚ਥߥ∔∛ߪ㧘ࠗࡦࡈ࡞ࠛࡦࠩ᭽∔ᖚ㧘ᗵᨴᕈ⢗⣺Ἳ㧘ή⩶ᕈ㜑⤑Ἳ㧘ᚻ⿷ญ∛㧘ࡋ࡞ࡄࡦࠡ࡯࠽ߢ޽ࠅ㧘ߘࠇߙࠇߩᬌ૕ߦߟ޿ߡᬌᩏࠍⴕߞߚ
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៝౉ᬌ૕ߣߘߩ࠙ࠗ࡞ࠬಽ㔌⁁ᴫࠍ⴫㧝ߦ␜ߒߚ㧚ᗵᨴ∝⊒↢േะ⺞ᩏ੐ᬺߪ 㧘‫ޟ‬ᗵᨴ∝ߩ੍㒐෸߮ᗵᨴ∝ߩᖚ⠪ߦኻߔࠆක≮ߦ㑐ߔࠆᴺᓞ‫ߦޠ‬ၮߠ៝౉ߐࠇߚᬌ૕ߪ∔∛ઙߢ៝౉ᬌ૕ᢙߪᤓᐕ

߈㧘ᗵᨴ∝ߩ⊒↢ࠍ੍㒐ߒ㧘ߘߩ߹ࠎᑧߩ㒐ᱛࠍߩઙߣ߶߷ห╬ߢ޽ߞߚ㧚⥃ᐥ⸻ᢿฬߏߣߦߺ

࿑ࠆߎߣࠍ⋡⊛ߦታᣉߐࠇߡ޿ࠆ㧚ᒰ⺖ߢߪ㧘หࠆߣ 㧘ࠗࡦࡈ࡞ࠛࡦࠩ᭽∔ᖚ߇ᬌ૕ߢᦨ߽ᄙߊ㧘੐ᬺߩ߁ߜ‫∛ޔ‬ේ૕ᖱႎߩឭଏࠍᜂᒰߒߡ㧘⋵ౝ೨ᐕߦᲧߴ⚂୚ߦჇടߒߚ㧚ᰴ޿ߢᗵᨴᕈ⢗⣺㧔⑔ጟᏒ㧘ർ਻ᎺᏒࠍ㒰ߊ㧕ߩ∛ේ૕ቯὐක≮ᯏἻ߇ઙ៝౉ߐࠇߚ㧚㑐ߦ߅޿ߡណขߐࠇߚᬌᩏ᧚ᢱߦߟ޿ߡ㧘࠙ࠗ࡞ㆊ෰ᐕ㑆ߩਥߥ∔∛ߏߣߩቯὐ޽ߚࠅߩᖚ⠪ႎࠬᬌᩏࠍⴕߞߡ޿ࠆ㧚ߎߎߢߪ‫ ޔ‬2004 ᐕߩᬌᩏ⚿๔ᢙߣ࠙ࠗ࡞ࠬဳࠍ࿑㧙ߦ␜ߒߚ 㧚ᖚ⠪ႎ๔ᢙߪ‫ޔ‬ᨐߦߟ޿ߡ␜ߔ㧚⑔ጟ⋵⚿ᩭ࡮ᗵᨴ∝⊒↢േะ⺞ᩏ੐ᬺ⾗ᢱ㓸ᐔᚑᐕ㧔ᐕ㧕ߦၮߠ޿ߡ࿑␜ߒߚ㧚ᐕߩ․ᓽࠍ

㧞␜ߔߣ㧘ࠗࡦࡈ࡞ࠛࡦࠩ᭽∔ᖚߩઙ߆ࠄ#*ဳ߇ᣇᴺ⋵ౝ 16 ࠞᚲߩ∛ේ૕ቯὐක≮ᯏ㑐ߢណขߐࠇ㧘ಽ㔌ߐࠇ㧘$ဳߪઙ߆ࠄߩߺߢ޽ߞߚ㧚߹ߚ㧘ή⩶

଻ஜ⑔␩ⅣႺ੐ോᚲߦࠃࠅ៝౉ߐࠇߚ♮ଢ㧘ຜ㗡ߧᕈ㜑⤑Ἳߩ∛ේ࠙ࠗ࡞ࠬߪ㧘೨ࠪ࡯࠭ࡦߩࠦࠢࠨ࠶

ߋ޿ᶧ㧘㜑ᶧ╬ߩᬌ૕ 305 ઙࠍ࠙ࠗ࡞ࠬᬌᩏ᧚ᢱߣ

ࠠ࡯㧔%QZ㧕$⟲࠙ࠗ࡞ࠬ߆ࠄࠛࠦ࡯࠙ࠗ࡞ࠬဳ㧘ߒߚ 㧚࠙ࠗ࡞ࠬಽ㔌ߪ㧘Vero㧘FL㧘RD18S㧘HEp-2㧘

ဳߦᄌൻߒߡ޿ߚ㧚ߒ߆ߒ㧘ᖚ⠪⊒↢ᢙߪ೨ᐕߣหMDCK ߩ 5 ⒳㘃ߩၭ㙃⚦⢩ߣ㧘↢ᓟ 48 ᤨ㑆ౝߩ੃ߓߊዊⷙᮨߢ޽ߞߚ㧚ࡋ࡞ࡄࡦࠡ࡯࠽߆ࠄߪ㧘%QZ#ߩߺࡑ࠙ࠬࠍ↪޿ߡⴕߞߚ㧚ಽ㔌࠙ࠗ࡞ࠬߪ㧘ਛ๺ဳ߇ਥߦಽ㔌ߐࠇ㧘೨ᐕߩ%QZ#ဳ߆ࠄਥᵹⴕᩣ߇

ⴊᷡࠍ↪޿ߚหቯࠍⴕߞߚ߇㧘หቯ࿎㔍ߥ႐วߦߪ

ᄌൻߒߡ޿ߚ㧚ᚻ⿷ญ∛ߦߟ޿ߡߪ㧘ᐔᐕਗߺߩᵹ

RT-PCR ᴺ ෸߮Ⴎၮ㈩೉ߩ᳿ቯߦࠃࠆหቯ߽⹜ߺ

ⴕߢߪ޽ߞߚ߇㧘ㅢᏱᵹⴕߩࡇ࡯ࠢ߇㧙᦬ߦߪߞ

ߚ㧚߹ߚ㧘ᗵᨴᕈ⢗⣺Ἳߦ㑐ߒߡߪ㧘Ꮢ⽼ࠗࡓࡁࠢ߈ࠅ⃻ࠇࠆߩߦኻߒ㧘ᧄᐕߪ᦬ߦᖚ⠪ႎ๔ᢙ߇਄᣹

ࡠࡑ࠻ᴺࠠ࠶࠻ߦࠃࠆࡠ࠲࡮ࠕ࠺ࡁ࠙ࠗ࡞ࠬ᛫ේߩ

ߒߚᓟ‫৻߷߶ޔ‬ቯߩ࡟ࡌ࡞ߢ᦬߹ߢ⛽ᜬߐࠇߚߎ

ᬌ಴ߣ RT-PCR ᴺ ߦࠃࠆࡁࡠ࠙ࠗ࡞ࠬㆮવሶߩᬌ

ߣ߇․ᓽߣߒߡ޽ߍࠄࠇࠆ㧚ಽ㔌ߐࠇߚߩߪ㧘%QZ#

಴ࠍⴕߞߚ㧚

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論文・学会等への発表㧔㧝㧕⺰ᢥ╬⊒⴫৻ⷩ
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㧖 1 Kyushu Women's University
㧖 2 Kyushu University
㧖 3 National Institute of Health Science㧖 4 Naogoya City Universtiy↲⁁⣼ᧃࠍ฽᦭ߔࠆஜᐽ㘩ຠਛߩ' ᫪↰㇌ᱜ㧘Ძ೑㓉⟤㧘ਛᎹ␞ሶ⑔ ጟ ⋵ ଻ ஜ Ⅳ Ⴚ ⎇ ⓥ ᚲ ᐕ P  -࠻࡝࡛࡯࠼࠴ࡠ࠾ࡦ෸߮࠴ࡠࠠࠪࡦ

ႎ㧘╙ภ㧘-㧘

ߩ HPLC ಽᨆᴺஜᐽ㘩ຠਛߩ࡝ࠝ࠴ࡠ࠾ࡦ࠽࠻࡝࠙ࡓ ᫪↰㇌ᱜ㧘Ძ೑㓉⟤㧘ਛᎹ␞ሶ⑔ ጟ ⋵ ଻ ஜ Ⅳ Ⴚ ⎇ ⓥ ᚲ ᐕ P ෸߮࡟ࡏ࠴ࡠࠠࠪࡦ࠽࠻࡝࠙ࡓߩ

ႎ㧘╙ภ㧘-㧘

HPLC ಽᨆᴺ
࠳ࠗࠠࠪࡦ㘃࠺࡯࠲ࡌ࡯ࠬߩ᭴▽ߣᳪ ጤᧄ⌀ੑ㧘᧻ᨑ㓉ᒾ㧘㤥Ꮉ㓁৻㧘ᄢ㊁ ⅣႺൻቇ㧘Vol㧘No㧘 P ᨴ⁁ᴫߩ⸃ᨆ
ஜᴦ㧘㘧⍹๺ᄢ㧘᪉ᧁᑪᴦ-

Preparation of activated carbon fibers from W.M.Qiao 㧖, S.H.Yoon 㧖, Y.Korai 㧖, Carbon,  -

P 

I.Mochida 㧖, S.Inoue 㧖, T.Sakurai 㧖, 

polyvinyl chloride.

T.Shimohara㧖 ਻Ꮊᄢቇ㧖 ࡝ࠨࠗࠢ࡞✚ว⎇ⓥ࠮ࡦ࠲࡯
Reaction

of

NO2

in

air

at

room N.Shirahama 㧖, I.Mochida 㧖,Y.Korai 㧖, Applied Catalysis

temperature with urea supported on pitch K.H.Choi
based activated carbon fiber.

㧖

A.Yasutake, T.Enjoji

㧖

P 

, T.Shimohara, B: Environmental 
-  㧖㧖 ਻Ꮊᄢቇ㧖 ૒⾐⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯㧖 ਃ⪉㊀Ꮏ 㐳ፒ⎇ⓥᚲណ᳓↪ེ᧚ߦ↱᧪ߔࠆ㋦ᳪᨴ੐଀⍹ᯅⲢሶ㧘᧻የ
ᢕሶ㧘਄↰ብ㧘ਛ᧛෶ༀ㧘╣የ ⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ P ୃ㧖㧖 ⑔ጟ⋵╳⚡଻ஜ⑔␩ⅣႺ੐ോᚲ ╙ภ㧘-㧘

⺰

ᢥ

ฬ

ၫ

╩

⠪

ឝ

タ

⹹

ᛞ㍳ឝタ㗁

Radon and Thoron Exposures for Cave S.Tokonami 㧖  , Q.Sun 㧖  , S.Akiba 㧖  , Radiation Research㧘ResidentsinShanxiandP Shaanxi W.Zhuo 㧖, M.Furukawa 㧖, T.Ishikawa 㧖, -㧘

Provinces

C.Hou 㧖, S.Zhang 㧖, Y.Narazaki,
B.Ohji 㧖, H.Yonehara 㧖and Y.Yamada 㧖㧖 ᡼኿✢කቇ✚ว⎇ⓥᚲ㧖 ᡼኿✢㒐⼔⎇ⓥᚲ㧖 㣮ఽፉᄢቇ

Association of manganese effluent with Yuko Ishibashi, Hiroshi Matsuo, Yoshiteru Water Research㧘P 

the application of fertilizer and manure on Baba, Yoshitaka Nagafuchi, Toshihiko -㧘
Imato 㧖, Tatemasa Hirata 㧖

tea field

㧖 1 Kyushu Unibersity
㧖 2 Wakayama Universityඳᄙḧߦ߅ߌࠆࡑࠢࡠࡌࡦ࠻ࠬࠍ⠨ᘦ ᾢ⼱ඳผ㧘ጊፒᗅ⟵㧖㧘ᷰㄝ੫৻㧖㧘⮮ ⅣႺᎿቇ⎇ⓥ⺰ᢥ㓸㧘ߒߚ᳓⾰⸃ᨆ
↰ஜ৻㧖╙Ꮞ㧘-㧘㧖 ⑔ጟᄢቇ㧖 ਻ᎺⅣႺ▤ℂදળP ቟ቯဳ↥ᬺᑄ᫈‛ᦨ⚳ಣಽ႐ߩᾲ⿒ᄖ ࿯↰ᄢテ㧘ዊችືᐔ 㧖㧘ਛጊ⵨ᢥ 㧖 㧘㜞 ㇺᏒᷡ᝹㧘Vol㧘✢ߦࠃࠆ⋙ⷞᚻᴺߩᬌ⸛
ᯅᶈม㧘ቝㇺችᓃ㧘ፉጟ㓉ⴕ㧖
0Q㧘RR㧘㧖 ਻Ꮊᄢቇ
㧚

P 

┻὇ࠍᷙวߒߚࠦࡦࠢ࡝࡯࠻ߩ᳓⾰ᵺ ࿯↰ᄢテ㧘⍹ᯅⲢሶ㧘ᓼ᳗㓉ม 㧖 㧘਎ ో࿖ⅣႺ⎇ળ⹹㧘Vol㧘 P 
No㧘pp-㧘㧚
ൻ․ᕈ
೑᩵৻㧖㧘ୖንિ৻㧖㧖 ⑔ጟ⋵࡝ࠨࠗࠢ࡞✚ว⎇ⓥ࠮ࡦ࠲࡯㧖 ⑔ጟ⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯ࠗࡦ࠹࡝ࠕ⎇ⓥᚲ㧖 ߊࠄ᦭㒢ળ␠࿯ფ⚦⩶ฌ⹏ଔᴺߩ᭴▽㧙ᑄ᫈‛ಣಽ ⼱ญೋ⟤㧖㧘⑔↰๺ᱜ㧖㧘₺႐ߩ⎫ൻ᳓⚛ࠟࠬ⊒↢ኻ╷ߩߚ߼ߦ㧙ጤ㧖㧘ጊ ↥ᬺක⑼ᄢቇ㔀⹹㧘ౝ๺♿ 㧘Ꮢේ೰ᔒ 㧘᳓㊁ᐽᐔ㧖㧘⍹ -㧘㧚㧖㧖᧻⛽਎ 㧖 㧘਎⦟ᥰਯ㧘ựፒశብ㧘㜞ᯅᶈม㧘ၳᎹ๺⟤㧖 ↥ᬺක⑼ᄢቇ
㧖 ർ਻ᎺᎿᬺ㜞╬ኾ㐷ቇᩞ⸘㧔⺰ᢥ╬⊒⴫৻ⷩ㧕

 ઙ

 

 㧘P 

㧔㧞㧕⊒⴫⺰ᢥᛞ㍳ 㑐▵⣧ౝᵈ኿ߦࠃࠆ㤛⦡ࡉ࠼࠙⃿⩶㓸࿅ᗵᨴ੐଀㧙 Association between chemical properties and oxidative⑔ጟ⋵damage due to nitorophenanthrenes and their related

⽷ᵤ⵨৻ 㧘ၳᎹ๺⟤㧘㊁↰ᄙ⟤ᨑ㧘↰ઍᓞሶ 㧦∛

compounds in primary rat hepatocytesේᓸ↢‛ᬌ಴ᖱႎ㧘   - 

Nobuyuki Sera Hiroshi Tokiwa 㧖  Hideo Utsumi 㧖 㧖㧖ᐕ᦬⑔ጟ⋵ౝߩ∛㒮ߢ㧘ᄌᒻᕈ㑐▵∝ᖚ⠪ߦ

Shigeki Sasaki 㧖 Kiyoshi Fukuhara 㧖  and Naoki Miyata 㧖 ኻߔࠆ㑐▵⣧ౝᵈ኿ߦࠃࠅ㧘㤛⦡ࡉ࠼࠙⃿⩶ߩ㓸࿅ᗵ㧦 Polycyclic aromatic compounds  -  -ᨴ߇⊒↢ߒߚ㧚ᵈ኿ߩḰ஻෸߮ᣉⴚߦ㑐ਈߒߚකᏧ෸

߮⋴⼔Ꮷߦߟ޿ߡ⺞ᩏߒߚ⚿ᨐ㧘ฬߩ⋴⼔Ꮷ߇Ḱ஻ᮡ⊛⤳ེ߇⢖ߢ޽ࠆߎߣ߇᣿ࠄ߆ߣߥߞߡ޿ࠆ⊒߇ߒߚᵈ኿ᶧࠍ૶↪ߒߚᖚ⠪ߩߺ߇⊒∝ߒߡ޿ࠆߎߣ߇

ࠎ‛⾰࠾࠻ࡠࡈࠚ࠽ࡦࠬ࡟ࡦ෸߮ߘߩ⺃ዉ૕ࠍ࡜࠶࠻

್᣿ߒߚ㧚ߐࠄߦ㑐ଥ⠪ߩ㥦⣧㧘ᚻᜰ╬߆ࠄᬌ಴ߐࠇ

ೋઍ⢄⤳⚦⢩ߦធ⒳ߒߡ㉄ൻ⊛៊்ᐲࠍ᷹ቯߒߚ㧚ߘߚ㤛⦡ࡉ࠼࠙⃿⩶ߣᖚ⠪߆ࠄᬌ಴ߐࠇߚ㤛⦡ࡉ࠼࠙⃿ߩ⚿ᨐ㧘ࡈࠚ࠽ࡦࠬ࡟ࡦ⺃ዉ૕ߦࠃࠆ㉄ൻ⊛៊்ᐲߩ⩶ߦߟ޿ߡࡄ࡞ࠬࡈࠖ࡯࡞࠼ࠥ࡞㔚᳇ᵒേߦࠃࠆᜰᮡߩ㧝ߟߢ޽ࠆ-hydroxydeoxyguanosine -OH-dG ߩ

DNA ⸃ᨆࠍⴕߥߞߚ㧚ߘߩ⚿ᨐ㧘ฬߩ⋴⼔Ꮷ߇ߘࠇ↢ᚑߪ㧘㔚ሶㆶర㔚૏㧘ᦨૐⓨ゠㆏ LUMO ߥߤߩߙࠇ㑐ਈߒߚᖚ⠪ߣ DNA ࡄ࠲࡯ࡦ߇৻⥌ߒߡ޿ߚ㧚ൻቇ⊛࠺࡯࠲ߣ㑐ㅪࠍ␜ߒߚ㧚ߎߩߎߣ߆ࠄ㧘ࡈࠚࡦ∉ቇ෸߮⚦⩶ቇ⊛⺞ᩏߩ⚿ᨐ߆ࠄ㧘㑐▵⣧ౝᵈ኿⺞ᢛ࠽ࡦࠬ࡟ࡦ⺃ዉ૕ߦࠃࠆ-OH-dG ߪ⟎឵ߒߡ޿ࠆ࠾࠻

ᤨߦ㤛⦡ࡉ࠼࠙⃿⩶߇ᳪᨴߒߚߎߣ߇್᣿ߒߚ㧚ߎߩࡠၮߩㆶర⊛ઍ⻢ߩߐࠇ߿ߔߐߣ⋧㑐ߔࠆߎߣ߇␜ໂ

ᴦ≮ߪ৻⥸ක≮ᯏ㑐ߢᐢߊⴕߥࠊࠇߡ߅ࠅ㧘ኻ⽎⠪߽ߐࠇߚ㧚ᄙߊ㧘ౣ⊒㒐ᱛߩෳ⠨ߣߔࠆߚ߼᭎ⷐࠍႎ๔ߒߚ㧚㧖 ਻Ꮊᅚሶᄢቇ㧖 ቬ௝଻ஜ⑔␩ⅣႺ੐ോᚲ㧖  ਻Ꮊᄢቇ㧖 ↰Ꮉ଻ஜ⑔␩ⅣႺ੐ോᚲ㧖  ࿖┙ක⮎ຠ㘩ຠⴡ↢⎇ⓥᚲ㧖  ฬฎደᏒ┙ᄢቇ

 Evaluation of Clastogenicity of Isoprothiolane and ↲⁁⣼ᧃࠍ฽᦭ߔࠆஜᐽ㘩ຠਛߩ ࠻࡝࡛࡯

Pyroquilon Using The Mouse Micronuclei Test࠼࠴ࡠ࠾ࡦ෸߮࠴ࡠࠠࠪࡦߩ HPLC ಽᨆᴺ

O. Nagafuchi㧖 K. Murakami,

Environ. Chem., -

M. Ukita㧖᫪↰㇌ᱜ㧘Ძ೑㓉⟤㧘ਛᎹ␞ሶ㧦⑔ጟ⋵଻ஜⅣႺ⎇ⓥ

㧦 Toxicol.

ᚲᐕႎ㧘╙ภ㧘-㧘

ࡑ࠙ࠬᧃ᪳ⴊዊᩭ⹜㛎ࠍ↪޿ߡ㧘Isoprothiolane ෸ ߮ㅒ⋧♽ߩ㜞ㅦᶧ૕ࠢࡠࡑ࠻ࠣ࡜ࡈࠍ૶ߞߡ㧘↲⁁⣼ߩ ㆮવሶ៊்ᕈߩ᦭ήࠍᬌ⸛ߒߚ㧚↪޿ᧃࠍ฽᦭ߔࠆஜᐽ㘩ຠਛߩ 3,3',5-࠻࡝࡛࡯࠼࠴ࡠ࠾ࡦߚ ⹜ᢱ ߪߎ ࠇ ࠄߩൻ ቇ ‛⾰ ߩᏒ ⽼⹜⮎ 㧘߅ࠃ ߮ߘࠇ෸߮࠴ࡠࠠࠪࡦߩಽᨆᴺࠍᬌ⸛ߒߚ㧚ஜᐽ㘩ຠߪࡔ࠲Pyroquilonࠄ ࠍ♖ ⵾ߒ ߚ ߽ߩߢ ޽ ࠆ㧚 ߘߩ ⚿ᨐ㧘 Ꮢ⽼⹜ ⮎ࠍᛩࡁ࡯࡞ ෸߮᳓㧛ࡔ࠲ࡁ࡯࡞㧔 1:1㧕 ࠍ↪޿ߡᵞᵺᓟ㧘ਈ ߒ ߚ ࡑ ࠙ࠬ ߢߪ 㧘 ᧃ᪳ⴊ ਛߩዊ ᩭࠍ᦭ ߔࠆ✂⁁ ⿒

Pronase ࠍ↪޿ߡട᳓ಽ⸃ߒߚ㧚 3,3',5-࠻࡝࡛࡯࠼࠴ࡠ

ⴊ⃿ᢙ߇㧘ࠦࡦ࠻ࡠ࡯࡞ߣᲧセߒ᦭ᗧߦჇടߒߚߩߦ

࠾ࡦ෸߮࠴ࡠࠠࠪࡦߪ 2 㧑ࠕࡦࡕ࠾ࠕ᳓ࡔ࠲ࡁ࡯࡞ṁኻߒ㧘♖⵾ߒߚ⹜ᢱߢߪ㧘ዊᩭࠍ᦭ߔࠆ✂⁁⿒ⴊ⃿ߩᶧߢ᛽಴ߒ㧘࿕⋧᛽಴ᴺ (Bond Elut C18 ࠞ ࡯࠻࡝࠶ࠫ )Ⴧ ട ߪ ⹺ ߼ ࠄ ࠇ ߥ ߆ ߞ ߚ 㧚 ߎ ߩ ߎ ߣ ߆ ࠄ㧘ࠍ↪޿ߡࠢ࡝࡯ࡦࠕ࠶ࡊߒߚ㧚㜞ㅦᶧ૕ࠢࡠࡑ࠻ࠣ࡜

Isoprothiolane ෸ ߮ Pyroquilon ߩ ㆮવሶ៊்ᕈߦ㑐ߒࡈߪࠞ࡜ࡓߦ InertsilODS-3 (4.6 x 150 mm, 5 µm)ࠍ 㧘⒖

ߡ ߪ㧘 ߎߩ ⹜ 㛎♽ߢ ߪ 㒶ᕈ ߢ޽ ࠅ㧘Ქ ᕈ⹜㛎 ߦ↪޿േ⋧ߦ᳓㧛ࡔ࠲ࡁ࡯࡞㧛࡝ࡦ㉄㧔 500:500:1, v/v㧕 ࠍ↪

ࠆ ⹜ᢱ ߩ⚐ ᐲ ߪ㧘ߢ ߈ ߁ࠆ 㒢ࠅ 㜞⚐ᐲ ߩ߽ߩ ࠍ↪޿޿㧘᷹ቯᵄ㐳 230 nm ߢಽᨆߒߚ㧚⹜ᢱࠍࡔ࠲ࡁ࡯࡞

ߥߌࠇ߫ߥࠄߥ޿ߎߣ߇ౣ⏕⹺ߐࠇߚ㧚෸߮᳓㧛ࡔ࠲ࡁ࡯࡞ࠍ↪޿ߡᵞᵺߔࠆߎߣߦࠃࠅ౒ሽ㧖 ජ⪲⑼ቇᄢቇ

‛ ⾰ࠍലᨐ ⊛ߦ㒰 ෰ߔࠆߎ ߣ߇ߢ ߈ߚ㧚 ߹ߚ㧘 Bond㧖 ጊญᄢቇElut C18 ࠞ࡯࠻࡝࠶ࠫಣℂߔࠆߎߣߦࠃࠅ㧘ߐࠄߦ౒ሽ‛⾰ࠍ㒰෰ߔࠆߎߣ߇ߢ߈ߚ㧚ᧄᴺߦࠃࠆஜᐽ㘩ຠ
߆ࠄߩ 3,3',5-࠻࡝࡛࡯࠼࠴ࡠ࠾ࡦ෸߮࠴ࡠࠠࠪࡦߩ࿁
෼₸ߪ 70 㨪 80%㧘ቯ㊂ਅ㒢୯ߪ 3,3',5-࠻࡝࡛࡯࠼࠴
ࡠ࠾ࡦ߇ 0.8 µg/g㧘࠴ࡠࠠࠪࡦ߇ 1.4 µg/g ߢ޽ߞߚ㧚  ஜᐽ㘩ຠਛߩ࡝ࠝ࠴ࡠ࠾ࡦ࠽࠻࡝࠙ࡓ෸߮࡟ࡏ࠴ࡠ

 Preparation of activated carbon fibers from polyvinylࠠࠪࡦ࠽࠻࡝࠙ࡓߩ HPLC ಽᨆᴺ

chloride᫪↰㇌ᱜ㧘Ძ೑㓉⟤㧘ਛᎹ␞ሶ㧦⑔ጟ⋵଻ஜⅣႺ⎇ⓥ

W MQiao 㧖 SHYoon 㧖 YKorai 㧖 I Mochida 㧖 ᚲᐕႎ㧘╙ภ㧘-㧘

S Inoue 㧖 TSakurai 㧖  T Shimohara: Carbon ㅒ⋧♽ߩ㜞ㅦᶧ૕ࠢࡠࡑ࠻ࠣ࡜ࡈࠍ૶ߞߡ㧘ஜᐽ㘩- 

ຠਛߩ࡝ࠝ࠴ࡠ࠾ࡦ࠽࠻࡝࠙ࡓ෸߮࡟ࡏ࠴ࡠࠠࠪࡦ࠽

ᑄࡐ࡝Ⴎൻࡆ࠾࡞ (PVC)ࡊ࡜ࠬ࠴࠶ࠢߪႮ⚛ᚑಽࠍ࠻࡝࠙ࡓߩಽᨆᴺࠍᬌ⸛ߒߚ㧚ஜᐽ㘩ຠਛߩ࡝ࠝ࠴ࡠ฽ ߻ߚ ߼㧘 ὾ ළᤨߦ ࠳ ࠗࠝ ࠠࠪ ࡦࠍ⊒ ↢ߔࠆ ߣ⸒ࠊ࠾ࡦ࠽࠻࡝࠙ࡓ෸߮࡟ࡏ࠴ࡠࠠࠪࡦ࠽࠻࡝࠙ࡓߪ 0.1

ࠇߡ޿ࠆ㧚ᧄ⎇ⓥߢߪ㧘 PVC ࠍ 2 Ბ㓏ടᾲಣℂߔࠆ

㧑㈶㉄ࡔ࠲ࡁ࡯࡞ࠍ↪޿ߡ᛽಴ߒ㧘࿕⋧᛽಴ᴺ (Bondߎ ߣߢ 㧘ࡇ ࠶ ࠴߆ࠄ ല ᨐ⊛ ߦ⣕ Ⴎ⚛ൻ ߔࠆᣇ ᴺࠍᬌࠞ࡯࠻࡝࠶ࠫ )ࠍ↪޿ߡ㧘 2 㧑ࠕࡦࡕ࠾ࠕ

⸛ߒߚ㧚ߘߩ⚿ᨐ㧘⣕Ⴎ⚛ൻߒߚエൻὐ 218 ͠ߩࡇ᳓ -ࡔ࠲ࡁ࡯࡞ṁᶧߢࠢ࡝࡯ࡦࠕ࠶ࡊߒߚ㧚㜞ㅦᶧ૕࠶ ࠴ ࠍ ⺞ᢛ ߒ ߚ 㧚⺞ ᢛߒ ߚࡇ ࠶࠴ ߪ⚜ ♻ߒ 㧘 ὇ൻ㧘ࠢࡠࡑ࠻ࠣ࡜ࡈߪࠞ࡜ࡓߦ InertsilODS-3 (4.6 x 150 mm,

⾮ᵴߩಣℂࠍ⚻ߡ㧘㜞ᵴᕈ὇⚛❫⛽ (ACF)ࠍ⵾ㅧߒߚ㧚5 µm)ࠍ 㧘 ⒖ േ ⋧ ߦ ᳓ 㧛 ࠕ ࠮ ࠻ ࠾ ࠻ ࡝ ࡞ 㧛 ㈶ ㉄⵾ㅧߒߚ ACF ߦߟ޿ߡ SO2 ߩᵺൻ⢻ߦߟ޿ߡ੍஻⹜Elut Certify

㧔 650:350:5㧘 v/v㧕ࠍ↪޿㧘᷹ቯᵄ㐳 230 nm ߢಽᨆߒ㛎ߒߚ⚿ᨐ㧘ᓥ᧪ߩ ACF ߦ඘ᢜߔࠆᵺൻ⢻ࠍ᦭ߔࠆߚ㧚⹜ᢱ᛽಴ᶧࠍ Bond Elut Certify ࠞ ࡯࠻࡝࠶ࠫಣℂ

ߎߣ߇ಽ߆ߞߚ㧚ߔࠆߎߣߦࠃࠅലᨐ⊛ߦ౒ሽ‛⾰ࠍ㒰෰ߔࠆߎߣ߇ߢ㧖 ਻Ꮊᄢቇ

߈ߚ㧚ᧄᴺߦࠃࠆஜᐽ㘩ຠ߆ࠄߩ࡝ࠝ࠴ࡠ࠾ࡦ࠽࠻࡝㧖 ࡝ࠨࠗࠢ࡞✚ว⎇ⓥ࠮ࡦ࠲࡯࠙ࡓ෸߮࡟ࡏ࠴ࡠࠠࠪࡦ࠽࠻࡝࠙ࡓߩ࿁෼₸ߪ 77 㨪
82%㧘 ቯ ㊂ ਅ 㒢 ୯ ߪ ࡝ ࠝ ࠴ ࡠ ࠾ ࡦ ࠽ ࠻ ࡝࠙ ࡓ ߇ 0.5µg/g㧘࡟ࡏ࠴ࡠࠠࠪࡦ࠽࠻࡝࠙ࡓ߇ 0.7 µg/g ߢ޽ߞߚ㧚 ࠳ࠗࠝࠠࠪࡦ㘃࠺࡯࠲ࡌ࡯ࠬߩ᭴▽ߣᳪᨴ⁁ᴫߩ⸃ Reaction of NO2 in air at room temperature with urea

ᨆsupported on pitch based activated carbon fiber.

ጤᧄ⌀ੑ㧘᧻ᨑ㓉ᒾ㧘㤥Ꮉ㓁৻㧘ᄢ㊁ஜᴦ㧘㘧⍹๺ᄢ㧘

N Shirahama 㧖   I Mochida 㧖   Y Korai 㧖   K H ᪉ᧁᑪᴦ㧦ⅣႺൻቇ㧘 Vol㧘 No㧘- 㧘

Choi 㧖   T Enjoji 㧖   T Shimohara A  Yasutake 㧖  :࠳ࠗࠝࠠࠪࡦ㘃ߩ࠺࡯࠲ࠍ㓸⚂ߒ㧘⸃ᨆߔࠆߚ߼ߦ

Applied Catalysis B: Environmental   - 

࠺࡯࠲ࡌ࡯ࠬࠍ᭴▽ߒߚ㧚࠺࡯࠲ࡌ࡯ࠬߪ㧘ណข࿾ὐ㧘ᚭᄖᄢ᳇ߦ߅ߌࠆ NO2 ߩㆶర෻ᔕߦߟ޿ߡᬌ⸛ߔ

ណขᣣᤨߥߤߩઃᏪ㗄⋡㧘⇣ᕈ૕㗄⋡㧘หᣖ૕㗄ࠆ ߚ ߼ ߦ 㧘 ቶ ᷷ ߢ 㧘 ዩ ⚛ ࠍ ᜂ ᜬ ߒ ߚ ACF ߦ 50 㨪⋡㧘 TEQ ⇣ ᕈ૕Ớᐲߥߤߢ᭴ᚑߐࠇߡ߅ࠅ㧘ㆊ෰ߩ

1000ppm ߩ NO2 ࠍㅢ᳇ߔࠆታ㛎ࠍⴕߥߞߚ㧚ߘߩ⚿⺞ᩏߢಽᨆߐࠇߚࠨࡦࡊ࡞એ਄߇෼߼ࠄࠇߡ޿ࠆ㧚ᨐ㧘 NO2 ߪ ㆶర㧘ಽ⸃ߐࠇ㧘⓸⚛ࠟࠬߦᄌ឵ߐࠇࠆ

ߎߩ࠺࡯࠲ࡌ࡯ࠬ߆ࠄ⸃ᨆߒߚ޿ࠣ࡞࡯ࡊࠍᛮ߈಴ߒ㧘ߎߣ߇⏕⹺ߢ߈ߚ㧚 ACF ਄ߦᜂᜬߒߚዩ⚛ߪ㧘௖߆⇣ᕈ૕ࡄ࠲࡯ࡦ㧘หᣖ૕ࡄ࠲࡯ࡦ㧘ㆬᛯߒߚᚑಽߩ࡟ߦ ᶖ ⾌ ߐࠇ ࠆ ෻ ᔕߢ ޽ࠆ ߎߣ ߇ಽ ߆ߞ ߚ㧚 ߎ ߩᤨ㧘࡯࠳࡯࠴ࡖ࡯࠻㧘 TEQ ᭴ ᚑߥߤߩࠣ࡜ࡈࠍ૞ᚑߔࠆ㧚ዉ౉ߔࠆࠟࠬਛߩ㉄⚛Ớᐲߪ㧘 NO2 ߩ ㆶర㧘ಽ⸃⢻߹ߚ㧘࠳ࠗࠝࠠࠪࡦ㘃ߩ࿯ფ㧘ᄢ᳇ߥߤߩᳪᨴ࿾ၞಽߦ ᓇ 㗀 ࠍ෸ ߷ ߒ ߡ޿ ߥ߆ ߞߚ 㧚⋧ ኻḨ ᐲߩ ਄ ᣹ߪ㧘Ꮣࠍߺࠆߚ߼ߦ⋵࿾࿑ߩ਄ߦ౞ࠣ࡜ࡈߢ⴫␜ߒ㧘ߘߩ

ACF ਄ ߢ NO2 ࠍ ⎣ ㉄ߦ ㉄ൻ ߐߖ ߿ ߔߊߔ ࠆ ߚ߼㧘㆑޿ࠍⷰኤߔࠆ㧚߹ߚ㧘⛔⸘࠰ࡈ࠻㧘ࠤࡒࠞ࡞ࡑࠬࡃ

NO2 ߩ ㆶర㧘ಽ⸃ߩኼ๮ࠍૐਅߐߖࠆߎߣ߇ಽ߆ߞ࡜ ࡦࠬ CMB ⸘▚࠰ࡈ࠻ࠍ૶޿㧘⊒↢Ḯ⒳ߩផቯ߿

ߚ㧚ነਈ₸ߩ੍᷹⸘▚ࠍⴕ߁㧚ߎߩ࠺࡯࠲ࡌ࡯ࠬࠍၮߦ㧘㧖 ਻Ꮊᄢቇ

㊀࿁Ꮻಽᨆ㧘 CMB ᴺߢ⊒↢Ḯߩ੍᷹ࠍⴕߞߚߣߎࠈ㧘㧖 ૒⾐⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯

߅߅߻ߨૃߚ⚿ᨐߣߥࠅ㧘ᄢ᳇ߢߪᑄ᫈‛὾ළἹ߇㧖 ਃ⪉㊀Ꮏ 㐳ፒ⎇ⓥᚲ%એ਄㧘࿯ფ࡮ᴡᎹ᳓ߢߪ CNP㧘 PCP ߩㄘ⮎߇%એ
਄ነਈߒߡ޿ࠆߎߣ߇ផቯߢ߈ߚ㧚  ណ᳓↪ེ᧚ߦ↱᧪ߔࠆ㋦ᳪᨴ੐଀ Association of manganese effluent with the application⍹ ᯅⲢ ሶ㧘 ᧻ የof fertilizer and manure on tea fieldብ 㧘 ਛ᧛ ෶ༀ 㧘╣ የᢕ ሶ㧘਄↰㧖Yuko Ishibashiᶏ᳓⹜ᢱ߆ࠄⅣႺၮḰ୯㧔 mg/l㧕ࠍ⿥߃ࠆ㋦߇

Yoshitaka Nagafuchi Toshihiko Imato 㧖  and Tatemasaୃ 㧦⑔ጟ⋵଻ஜⅣႺ⎇ⓥᚲᐕႎ╙ภ㧘-㧘㧚ᬌ಴ߐࠇߚ㧚㋦߇ၮḰ୯ࠍ⿥ㆊߒߚේ࿃ࠍᬌ⸛ߒߚ⚿HiroshiMatsuo

Yoshiteru Baba

Hirata 㧖:Water Research  - 

ᨐ㧘ណ᳓ࠍⴕߞߚᆔ⸤ᬺ⠪߇૶↪ߒߡ޿ߚࡂࠗࡠ࡯࠻⨥ ⇌ ࠍ ⿠ Ḯ ߣ ߔ ࠆ ḝ ᳓ ߢ ࡑ ࡦ ࠟ ࡦ Ớ ᐲ ߇  -ណ᳓ེߩบᐳߩਛߩ߅߽ࠅ㧔㋦߇૶↪ߐࠇߡ޿ߚ㧕߇

mg/l ߣ 㜞޿୯ ࠍ␜ߒ ߚ㧚⨥⇌߆ࠄߩࡑࡦࠟࡦᵹ಴㊂ᳪᨴḮߢ޽ࠆߎߣ߇ࠊ߆ߞߚ㧚ߪ 㧘 g/ha㧔 ᐕ᦬ -ᐕ᦬㧕 㧘 g/ha㧖 ⑔ጟ⋵╳⚡଻ஜ⑔␩ⅣႺ੐ോᚲ㧔ᐕ᦬ -ᐕ᦬㧕ߣផ⸘ߐࠇߚ㧚߹ߚ㧘᪢㔎ᦼߦࡑࡦࠟࡦᵹ಴㊂߇ᄙ߆ߞߚℂ↱ߪ㧘᪢㔎ᦼ೨ߦ⢈ᢱࠍᣉ⢈ߔࠆߎߣߦࠃࠅ࿯ფ߇㉄ᕈൻߒ㧘࿯ფਛߩṁ⸃ᕈࡑࡦࠟࡦ߇Ⴧടߔࠆߚ߼ߢ޽ࠆߣ⠨߃ࠄࠇߚ㧚㧖 ਻Ꮊᄢቇ㧖 ๺᱌ጊᄢቇ

 Radon and Thoron Exposures for Cave Residents in ඳᄙḧߦ߅ߌࠆࡑࠢࡠࡌࡦ࠻ࠬࠍ⠨ᘦߒߚ᳓⾰⸃

Shanxi and Shaanxi ProvincesᨆSTokonami 㧖  QSun 㧖 SAkiba 㧖 WZhuo 㧖 Mᾢ⼱ඳผ㧘ጊፒᗅ⟵ 㧖㧘ᷰㄝ੫৻ 㧖㧘⮮↰ஜ৻ 㧖ඳᄙḧḧᅏߢఝභ⒳ߢ޽ࠆࡎ࠻࠻ࠡࠬࠟࠗࠍࡑࠢࡠ

Furukawa 㧖  T Ishikawa 㧖  CHou 㧖 SZhang 㧖 Y
Narazaki BOhji㧖 HYonehara㧖and Y Yamada

㧖

; 

ࡌࡦ࠻ࠬࠦࡦࡄ࡯࠻ࡔࡦ࠻ߣߒߡ⚵ߺㄟࠎߛ↢ᘒ♽ࡕ

Radiation Research  - 

࠺࡞ࠍ૞ᚑߒߚ㧚ᧄࡕ࠺࡞ߪࡑࠢࡠࡌࡦ࠻ࠬࠍ㑐ᢙߩ㜞᡼኿✢࿾ၞߢ޽ࠆਛ࿖5JCPZK෸߮ 5JCCPZK⋭ߦߺߣߒߡᛒߞߡ޿ࠆᓥ᧪ߩࡕ࠺࡞ࠃࠅ߽㧘⽺㉄⚛᳓႙޽ࠆ㤛࿯บ࿾ߩᚭߩᵢ─૑ዬߦߡ⥄ὼ᡼኿⢻ߩ᷹ߩ᜼േࠍ⹦⚦ߦᛠីߔࠆߎߣ߇น⢻ߢ޽ߞߚ㧚ߎߩߎቯࠍⴕߞߚ㧚ቶౝ࡜࠼ࡦỚᐲߪ㨪 $S O ߢᐞߣߪ㧘ᐩ⾰ਛߦ↢ᕷߒߡ޿ࠆࡑࠢࡠࡌࡦ࠻ࠬߪ㧘⽺㉄૗ᐔဋ୯ߪ $S O ቶౝ࠻ࡠࡦỚᐲߪ 㨪  $S⚛᳓႙ߩ᜼േࠍ੍᷹ߔࠆ↢ᘒ♽ࡕ࠺࡞ࠍ᭴▽ߔࠆ㓙ߩO ߢᐞ૗ᐔဋ୯ߪ $S O ߢ޽ߞߚ㧚ቶౝߩᐔⴧ

ήⷞߢ߈ߥ޿㊀ⷐߥ࿃ሶߢ޽ࠆߎߣࠍ␜ໂߒߡ޿ߚ㧚╬ଔ࠻ࡠࡦỚᐲߪ 㨪  $S O ߢᐞ૗ᐔဋ୯ߪ㧖 ⑔ጟᄢቇ

 $S O  ߢ޽ߞߚ㧚ⓨ㑆ǫ✢✢㊂₸ߩᐔဋ୯ߪቶ㧖 ਻ᎺⅣႺ▤ℂදળౝߢP)[ J ቶᄖߢP)[ J ߢ޽ߞߚ㧚☨࿖0CVK

QPCN %CPEGT +PUVKVWVGߩ⺞ᩏߢߪᒰ࿾ߩ⢖ࠟࡦ࡝ࠬࠢࠍ࠻ࡠࡦߪ᷹ቯߖߕߦ࡜࠼ࡦ࡟ࡌ࡞ߩ਄᣹ߦ઻޿
Ⴧടߒߚߣ⚿⺰ઃߌߡ޿ࠆ߇੹࿁ߩ⎇ⓥ߆ࠄ࠻ࡠࡦ
ߩᓇ㗀߇ᄢ߈ߊߘߩ㊀ⷐᕈ߇シߊ⷗Ⓧ߽ࠄࠇߚน⢻ᕈࠍᜰ៰ߒߚ㧚㧖 ᡼኿✢කቇ✚ว⎇ⓥᚲ㧖 ᡼኿✢㒐⼔⎇ⓥᚲ㧖 㣮ఽፉᄢቇ  ቟ቯဳ↥ᬺᑄ᫈‛ᦨ⚳ಣಽ႐ߩᾲ⿒ᄖ✢ߦࠃࠆ⋙ ࿯ფ⚦⩶ฌ⹏ଔᴺߩ᭴▽ 㧙ᑄ᫈‛ಣಽ႐ߩ⎫ൻ᳓

ⷞᚻᴺߩᬌ⸛

⚛ࠟࠬ⊒↢ኻ╷ߩߚ߼ߦ㧙࿯ ↰ᄢ テ㧘 ዊችືᐔ 㧘 ਛጊ⵨ ᢥ 㧘㜞 ᯅᶈม 㧘ቝㇺ⼱ญೋ⟤ 㧖㧘⑔↰๺ᱜ 㧖㧘₺ችᓃ㧘ፉጟ㓉ⴕ 㧖㧦 ㇺᏒᷡ᝹㧘 Vol㧘 No㧘೰ᔒ 㧖  㧘᳓㊁ᐽᐔ 㧖  㧘⍹᧻⛽਎ 㧖  㧘਎⦟ᥰਯ㧘ựፒశpp-㧘㧚ብ㧘㜞ᯅᶈม㧘ၳᎹ๺⟤㧦↥ᬺක⑼ᄢቇ㔀⹹㧘㧖㧖቟ቯဳ↥ᬺᑄ᫈‛ᦨ⚳ಣಽ႐ߦ߅޿ߡ㧘ᷙ౉ߒߚ᦭ጤ 㧖㧘ጊౝ๺♿ 㧖㧘Ꮢේ 㧘-㧘㧚ᯏ‛ߦ⿠࿃ߔࠆ⊒ᾲ⃻⽎ࠍᬌ಴ߔࠆߎߣࠍ⋡⊛ߣߒߡ㧘ᑄ᫈‛ಣಽ႐߿ਇᴺᛩ᫈⃻႐ߦ߅޿ߡࠟࠬ⊒↢߇ᄙᾲ⿒ᄖ✢↹௝ⵝ⟎ࠍ↪޿ߚ࿾⴫㕙᷷ᐲ⺞ᩏࠍⴕߞߚ㧚⊒ߒߡ޿ࠆ㧚⎫ൻ᳓⚛ࠟࠬ⊒↢੍᷹ߩၮ␆ߣߥࠆ࿯ფ

߹ߕ㧘ደᄖ੍஻ታ㛎ࠍⴕ޿㧘ᾲ⿒ᄖ✢↹௝ߩ᠟ᓇ᧦ઙ

⚦⩶ฌߩേᘒࠍ㊂⊛㧘⾰⊛ߦ⹏ଔߔࠆߚ߼ߩㆮવሶᎿ

ࠍᬌ⸛ߒߚ⚿ᨐ㧘࿾⴫㕙᷷ᐲߩ⺞ᩏߦㆡߒߚᤨ㑆Ꮺߪቇ⊛ᬌᩏᴺࠍ᭴▽ߔࠆߎߣࠍ⋡⊛ߦ㧘ᓥ᧪ߩᨴ⦡ᴺ㧘ᄛ᣿ߌ೨ߢ޽ࠆߎߣ߇᣿ࠄ߆ߦߥߞߚ㧚ᰴߦ㧘቟ቯဳ

ၭ㙃ᴺߦࠃࠆᬌ⸽ߣߣ߽ߦ㧘ታ㛎ᚻᴺߩ⏕┙ࠍⴕߞߚ㧚ಣಽ႐ߦ┙ߜ౉ࠅ㧘ᾲ⿒ᄖ✢↹௝ߩ᠟ᓇࠍⴕߞߚ⚿ᨐ㧘⎫ൻ᳓⚛ࠟࠬ⊒↢ߦ㑐ਈߔࠆࠗࠝ࠙ߩ㉄ൻ߹ߚߪㆶర

ၒ┙࿾ౝㇱߩ᦭ᯏ‛ಽ⸃෻ᔕߦࠃࠆ⊒ᾲ▎ᚲࠍ㧘࿾⴫⩶⟲ߩ㗫ᐲࠍ⺞ߴߚ⚿ᨐ㧘ਇᴺᛩ᫈⃻႐ߢߪή⧘⢩⎫㕙᷷ᐲಽᏓ߆ࠄផ᷹ߔࠆߎߣ߇น⢻ߢ޽ߞߚ㧚߹ߚ㧘㉄ㆶర⩶ߣࠗࠝ࠙㉄ൻ⚦⩶⟲߇㜞㗫ᐲߦᬌ಴ߐࠇ㧘ᑄࡋ࡝ࠦࡊ࠲࡯ߦࠃࠅಣಽ႐ో૕ࠍ਄ⓨ߆ࠄ᠟ᓇߔࠆ਄

᫈‛ಣಽ႐ߢߪ᦭⧘⢩⎫㉄ㆶర⩶߿ Clostridium ዻ ⩶ⓨ⺞ᩏࠍⴕߞߚ߇㧘ᣣ኿ߩᓇ㗀߇ᄢ߈߆ߞߚ㧚ߎࠇࠄ߇ᄙߊᬌ಴ߐࠇߚ㧚ㆮવሶᎿቇ⊛ᬌᩏᴺ߇࿯ფߩᓸ↢ߩ⚿ᨐ߆ࠄ㧘ᾲ⿒ᄖ✢↹௝ⵝ⟎ߦࠃࠆ⺞ᩏᣇᴺ߇㧘ಣ‛ฌ⹏ଔ߅ࠃ߮ಣಽ႐ߩࠟࠬ⊒↢੍⍮ߦ᦭↪ߢ޽ࠆߎಽ႐ߦ߅ߌࠆ᦭ᯏ‛ಽ⸃ࠍ⋙ⷞߔࠆᣇᴺߣߒߡ᦭ലߢ

ߣ߇␜ໂߐࠇߚ㧚޽ࠆߎߣ߇᣿ࠄ߆ߣߥߞߚ㧚㧖 ↥ᬺක⑼ᄢቇ

㧖 ਻Ꮊᄢቇ㧖 ർ਻ᎺᎿᬺ㜞╬ኾ㐷ቇᩞ ┻὇ࠍᷙวߒߚࠦࡦࠢ࡝࡯࠻ߩ᳓⾰ᵺൻ․ᕈ
࿯↰ᄢテ㧘⍹ᯅⲢሶ㧘ᓼ᳗㓉ม 㧖 㧘਎೑᩵৻ 㧖 㧘ୖንિ৻ 㧖㧦ో࿖ⅣႺ⎇ળ⹹㧘 Vol㧘 No㧘 pp-㧘㧚┻὇ࠍᷙวߒߚᴡᎹ⼔ጯ↪ࠦࡦࠢ࡝࡯࠻ࡉࡠ࠶ࠢࠍ
૞⵾ߒߚ㧚ߎߩࠦࡦࠢ࡝࡯࠻ࡉࡠ࠶ࠢߩ᳓⾰ᵺൻ․ᕈࠍ㧘ࠦࡦࠢ࡝࡯࠻ࡉࡠ࠶ࠢ߆ࠄṁ಴ߔࠆṁ⸃ᕈ‛⾰ߣ㧘ࠦࡦࠢ࡝࡯࠻ࡉࡠ࠶ࠢ⴫㕙ߦઃ⌕ߒߚᓸ↢‛ߦࠃࠆ᦭ᯏ‛ಽ⸃⢻ߩਔ㕙߆ࠄ⠨ኤߒߚ㧚ࠦࡦࠢ࡝࡯࠻⹜㛎ߦࠃࠆቶౝߢߩᶐẃታ㛎ߩ⚿ᨐ㧘┻὇ᷙวࠦࡦࠢ࡝࡯
࠻߆ࠄߪ㧘┻὇↱᧪ߩࠞ࡝࠙ࡓࠗࠝࡦ߿㧘૶↪ߒߚ․ᱶ࠮ࡔࡦ࠻↱᧪ߩࠕ࡞ࡒ࠾࠙ࡓࠗࠝࡦߥߤ߇㧘┻὇ߩ౉ߞߡ޿ߥ޿᥉ㅢࠦࡦࠢ࡝࡯࠻ߦᲧߴߡᄙߊṁ಴ߒߚ㧚߹ߚ┻὇ᷙวࠦࡦࠢ࡝࡯࠻ߪ㧘᥉ㅢࠦࡦࠢ࡝࡯࠻ߦᲧ
ߴ㧘౐ଔࠢࡠࡓߩṁ಴߇ዋߥߊ㧘ๆ⌕ታ㛎ߩ⚿ᨐ㧘┻὇ߦๆ⌕ߐࠇߚߎߣ߇ࠊ߆ߞߚ㧚⹜㛎ࠍ㉄ൻᳰߦ

ࡩ᦬㑆ᴉ߼ߡᓸ↢‛ࠍઃ⌕ߐߖߚ⚿ᨐ㧘᥉ㅢࠦࡦࠢ࡝
࡯࠻ߩ⚂୚㊂ߩᓸ↢‛߇ઃ⌕ߒߚ㧚ߎߩઃ⌕ᓸ↢‛㊂ߩ㆑޿ߦࠃࠅ㧘┻὇ᷙวࠦࡦࠢ࡝࡯࠻ߩ BOD 㒰෰ㅦᐲߪ㧘᥉ㅢࠦࡦࠢ࡝࡯࠻ࠃࠅᄢ߈߆ߞߚ㧚㧖 ⑔ጟ⋵࡝ࠨࠗࠢ࡞✚ว⎇ⓥ࠮ࡦ࠲࡯㧖 ⑔ጟ⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯ࠗࡦ࠹࡝ࠕ⎇ⓥᚲ㧖 ߊࠄ᦭㒢ળ␠ 

㧔㧟㧕ቇળ╬ญ㗡⊒⴫৻ⷩ
Ԙ࿖㓙ቇળ
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⴫

⠪

ቇળฬ㧔႐ᚲ㧕㧘ᐕ᦬ᣣ

Application of solvent cut large Kazuhiro Tobiishi Takashi Todaka Hironori 
SCLV injection system Hirakawa Tsuguhide Hori and Takao Iida

volumeth

International Symposium on

Halogenated Environmental Organic

using narrow-bore column to dioxins

Pollutants

and

POPs

(Berlin,analysis in human blood

Germany), September - 

Development of database system for Takahiko Matsueda, Shinji Iwamoto, Naoko China-Japan Joint symposium on
dioxinsand

its

application

to Kita, Kenji Ohno, Youichi Kurokawa, Kazuhiro Environmental Chemistry (Beijing,pollution analysis of dioxins in the Tobiishi, Daisuke Yasutake and Kenji Sakuragi

China), October - 

environment
Comparison of accelerated solvent Tsuguhide
extraction

andstandard

extraction

fordeterminationofthInternational Symposium on

shaking Nakagawa, Takao Iida, Tomoaki Tsutsumi*, Halogenated Environmental Organicof Kumiko Sasaki*

Pollutants

㧖 National Institute of Health Sciences

dioxins in foods
LevelsHori, Kazuhiro Tobiishi, Reiko 

and

POPs

(Berlin,Germany), September  - 

P o l y b r o m i n a t e d Yuki Ashizuka Reiko Nakagawa Tsuguhide thInternational Symposium on

Diphenyl-Ethers and Polybrominated Hori Kazuhiro Tobiishi and Takao Iida

Halogenated Environmental Organic

Dioxins in Fish Total Diet Study

Pol lut a nt s

Food Groups and Japanese Meals

Germany), September - 

Follow-up survey of dioxins and Takao Iida Hironori
related chemicals in the blood of Hori
Yusho patients in Takahiko

Hirakawa Tsuguhide 

MatsuedathPOPs( Be rli n,International Symposium on

Kazuhiro Halogenated Environmental Organic

Tobiishi Reiko Nakagawa Takashi Todaka 㧖 Pol lut a nt s
and Masutaka Furue

a nd

a nd

POPs( Be rli n,Germany), September - 

㧖㧖 Japan Food Hygiene Association
㧖 Kyushu University
Concentration and distribution of Takao

Iida Takashi Todaka 㧖   Hironori thInternational Symposium on

dioxins and related compounds in Hirakawa Tsuguhide Hori Kazuhiro Tobiishi Halogenated Environmental Organic
various human organs

TakahikoMatsueda

Taketo Yamada

Shau

Watanabe 㧖 and Pol lut a nt sa ndPOPs( Be rli n,Germany), September - 

㧖㧖 Japan Food Hygiene Association
㧖 Tokyo University of Agriculture㧖 Keio University
Inter-laboratory calibration study of Takao
dioxins in human blood samples⸘㧔࿖㓙ቇળ㧕

Iida

TakashiTodaka 㧖 

Hironori China-Japan Joint Symposium on

Hirakawa Tsuguhide Hori Kazuhiro Tobiishi E n v i r o n m e n t a l
Reiko Nakagawa and Takahiko Matsueda

Chemistry(Beijing,China) October㧖 Japan Food Hygiene Association

- ઙ 

ԙ࿖ౝቇળ㧔ో࿖㧕Ṷ㗴ฬᴤ∝ᖚ⠪ㅊ〔ᬌ⸻ߩᐔᚑᐕ

⊒

⴫

⠪

ቇળฬ㧔႐ᚲ㧕㧘ᐕ᦬ᣣጟᕶ৻㇢㧘↲ේ㓉⍫㧘ዊ㊁Ⴆᄢ੺㧘㜞ጊᶈ᮸ᐔᚑᐕᐲෘ↢ഭ௛⋭ో࿖ᴤ∝ᐲో࿖㓸⸘⚿ᨐႎ๔෸߮ᴤ∝ᴦ≮⎇ⓥ⃰ળ⼏㧔⑔ጟᏒ㧕㧘ᖚ⠪࠺࡯࠲ࡌ࡯ࠬ CD ߩ㈩ઃᐔᚑᐕ᦬ᣣ

ߦߟ޿ߡ⑔ጟ⋵ߩ㜞ᱫ੢₸࿾ၞߣૐᱫ

ጟᕶ৻㇢㧘ዊ㊁Ⴆᄢ੺㧘ศ᧛ஜᷡ╙࿁౏ⴐⴡ↢ᖱႎ⎇ⓥද⼏ળ੢₸࿾ၞߦ߅ߌࠆ↢ᵴ⠌ᘠ╬⎇ⓥળ㧔๺శᏒ㧕㧘⺞ᩏ⚿ᨐߦߟ޿ߡ

ᐔᚑᐕ᦬ᣣ

౏ⴐⴡ↢කᏧߩ⏕଻ᣇ╷ߣ౏ ศ᧛ஜᷡ╙࿁ᣣᧄ౏ⴐⴡ↢ቇળ✚ળⴐⴡ↢ߩะ਄ߦะߌߡ㧙ࠃࠅ㧔᧻ᳯᏒ㧕㧘ᐔᚑᐕ᦬ᣣ

ࠃ߈౏ⴐⴡ↢කᏧߩ㙃ᚑ㧙ᦨㄭ⹤㗴ߩᣂ⥝࡮ౣ⥝ᗵᨴ∝ ศ᧛ஜᷡ㧘ዊ㊁Ⴆᄢ੺㧘ጟᕶ৻㇢ߦ߅ߌࠆⴡ↢ቇ࡮౏ⴐⴡ↢ቇ╙࿁ᣣᧄⴡ↢ቇળ㧔ᣂẟᏒ㧕㧘ᐔᚑᐕ᦬ᣣ

⠪ߩᨐߚߔᓎഀ‫ⷞߩࠄ߆⋵ޟ‬ὐࠃࠅ‫ޠ‬
㜞ᵴᕈ὇⚛❫⛽ࠍ↪޿ߚⅣႺ ᣂ⼱ବੑ㧘᧼၂ᚑᵏ㧘ਅේቁ┨╙࿁ᄢ᳇ⅣႺቇળᐕળ㧔⑺↰ᄢ᳇ᵺൻߦ㑐ߔࠆ⎇ⓥ㧔㧕㆏Ꮢ㧕㧘ᐔᚑᐕ᦬-ᣣ〝ᴪ㆏ߦ߅ߌࠆ NO㧘NOᵺൻ⹜㛎㧙 ACF ⒳㧘ណ᳇ᵹㅦ㧘᷷Ḩᐲߣߩ㑐ଥ㧙ᑄ᫈‛ಣಽ႐ߦ߅ߌࠆ⎫㉄ㆶ ựፒశብ䋬ၳᎹ๺⟤䋬਎⦟ᥰਯ䋬㜞ᯅᶈม䋬⍹㤥㕏 ╙࿁᳓ⅣႺቇળ㧔ජ⪲Ꮢ 㧕㧘
ర⩶ߩᬌ಴ၭ࿾ߩᬌ⸛

ዏ䋬ቝㇺችᓃ

ᐔᚑᐕ᦬ᣣ

eaeA ㆮવሶࠍᬌ಴ߒߚᄢ⣺⩶ ᧛਄శ৻㧘㐳㊁⧷ବ㧖㧘ựፒశብ㧘ၳᎹ๺⟤㧘⍹ ╙࿁ᣣᧄ౏ⴐⴡ↢ቇળ✚ળ㧔᧻
Oߦࠃࠆ㘩ਛᲥ੐଀㤤㕏ዏ㧘㜞↰ೣሶ㧖㧘᪢ፒ⺈ᴦ㧖㧖 ↰Ꮉ଻ஜ⑔␩ⅣႺ੐ോᚲ✛㤛⦡㊁⩿ߦࠃࠆ-OH-dG ߩ ਎⦟ᥰਯ㧘⑔ේᛥ೙ലᨐẖ㧖㧘Ᏹ⋚

ᳯᏒ㧕㧘ᐔᚑᐕ᦬ᣣ㧖 ↢ᵴⴡ↢⺖ኡ㧖㧘ౝᶏ⧷㓶㧖㧘 ᣣᧄⅣႺᄌ⇣ේቇળ╙࿁ᄢળ૒‫ ⾆⨃ᧁޘ‬㧖㧘ਛ⷏ᵗ৻ 㧖㧘ች↰⋥᮸ 㧖㧘ᎂጾ੗ ෸߮ᣣᧄേ‛ታ㛎ઍᦧᴺቇળ╙ൎ㧖࿁ᄢળ㧔㐳ፒᏒ㧕㧘㧖 ࿖┙ක⮎ຠ㘩ຠⴡ↢⎇ⓥᚲ㧖 ਻Ꮊᅚሶᄢቇ㧖 ਻Ꮊᄢቇ㧖 ฬฎደᏒ┙ᄢቇ㧖 㕍᫪⋵┙଻ஜᄢቇ 

ᐔᚑᐕ᦬ᣣ

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ੱ૕⤳ེਛߩ࠳ࠗࠝࠠࠪࡦ㘃 ᚭ㜞
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⴫

⠪

ዅ 㧖 㧘ᐔᎹඳ઄㧘ၳ᧻ᨑ㓉ᒾ㧘ᷰㄝ᣽㧖ቇળฬ㧔႐ᚲ㧕㧘ᐕ᦬ᣣዞ⧷㧘㘧⍹๺ᄢ㧘 ╙࿁ᣣᧄⅣႺൻቇ⸛⺰ળ㧔㕒㧘ጊ↰ஜੱ㧖㧖 ᣣᧄ㘩ຠⴡ↢දળጟᏒ㧕㧘ᐔᚑᐕ᦬ᣣ-ᣣ㧖 ᧲੩ㄘᬺᄢቇ㧖 ᘮᔕ⟵Ⴖᄢቇ㘩ຠਛߩ࠹࠻࡜ࡉࡠࡕࡆࠬࡈ ⧃Ⴆ↱♿㧘ਛᎹ␞ሶ㧘ၳࠚࡁ࡯࡞ A TBBPAዞ⧷㧘㘧⍹๺ᄢ㧘㘵↰ ╙࿁ో࿖ⴡ↢ൻቇᛛⴚද⼏ળ

ಽᨆᴺ 㓉㓶

ᐕળ㧔↲ᐭᏒ㧕㧘ߩᬌ⸛ᐔᚑᐕᐕᣣ-ᣣ㘩ຠߦ߅ߌࠆ⥇⚛♽㔍Ά೷ߩ ⧃Ⴆ↱♿㧘ਛᎹ␞ሶ㧘ၳಽᨆዞ⧷㧘㘧⍹๺ᄢ㧘㘵↰ ⅣႺࡎ࡞ࡕࡦቇળ╙࿁⎇ⓥ⊒⴫㓉㓶ળ ฬฎደᏒ

㧘ᐔᚑᐕ᦬ᣣ-ᣣ
Follow-up surveyofdioxins ᚭ㜞ዅ 㧖 㧘ᐔᎹඳ઄㧘ၳዞ⧷㧘㘧⍹๺ᄢ㧘 ⅣႺࡎ࡞ࡕࡦቇળ╙࿁⎇ⓥ⊒⴫concentration in the blood of 㘵↰㓉㓶㧘ฎᳯჇ㓉㧖Yusho patients in -㧖 ᣣᧄ㘩ຠⴡ↢දળThe levels of PCDDsPCDFs ᐔᎹඳ઄㧘ᚭ㜞
Non-ortho

PCBs

a n d 㘵↰㓉㓶㧘ጯળ ฬฎደᏒ㧖 ਻Ꮊᄢቇዅ 㧖 㧘ၳ㧘ᐔᚑᐕ᦬ᣣ-ᣣዞ⧷㧘㘧⍹๺ᄢ㧘 ⅣႺࡎ࡞ࡕࡦቇળ╙࿁⎇ⓥ⊒⴫⃍ሶ 㧘૒↰ᢥብ㧖㧘⷏᧦ᵏ᣿㧖㧘 ળ ฬฎደᏒ㧖㧘Mono-ortho PCBs in the blood ട⮮㕒ᕺ 㧘ਛፉߘߩߺ 㧖㧘૒‫ᧁޘ‬ᚑሶ 㧖㧘㡻㊁ ᐔᚑᐕ᦬ᣣ-ᣣ㧖

of pregnant women቟Ꮧሶ㧖㧘ㄭ⮮᦮ሶ㧖㧘᫪ࠁ߁ߎ㧖㧖 ᣣᧄ㘩ຠⴡ↢දળ㘩ຠߦ߅ߌࠆ⥇⚛ൻࠫࡈࠚ࠾ ਛᎹ␞ሶ㧘⧃Ⴆ↱♿㧘ၳ㧖 ർᶏ㆏ᄢቇ
ዞ⧷㧘㘧⍹๺ᄢ㧘㘵↰ ᣣᧄ㘩ຠⴡ↢ቇળ╙࿁ቇⴚ⻠࡞ࠛ࡯࠹࡞෸߮⥇⚛ൻ࠳ࠗࠝ 㓉㓶Ṷળ㧔ᐢፉᏒ㧕㧘ࠠࠪࡦಽᨆᐔᚑᐕ᦬-ᣣ㜞ᵴᕈ὇⚛❫⛽ࠍዉ౉ߒߚㅢ ᧲ᶏᨋቁᐘ㧖㧘ർ↰ᢅᑝ㧖㧘ਅේቁ┨࿯ᧁቇળ╙࿁࿾⃿ⅣႺࠪࡦࡐ᳇ᕈㆤ㖸ოߩ⓸⚛㉄ൻ‛㒰෰ 㧖 ⼾ᯅᛛⴚ⑼ቇᄢቇ

ࠫ࠙ࡓ㧔ฬฎደᏒ㧕㧘ലᨐߦߟ޿ߡ㧙ᢙ୯ࡕ࠺࡞ߦ

ᐔᚑᐕ᦬ᣣ-ᣣࠃࠆᬌ⸛㧙㜞ᵴᕈ὇⚛❫⛽ࠍ↪޿ߚⅣႺ ਅේቁ┨㧘ജኼ㓶㧘᧼၂ᚑᵏ㧘ᣂ⼱ବੑᄢ᳇ ᵺൻ ߦ㑐ߔ ࠆ⎇ⓥ㧔 8㧕╙࿁ᄢ᳇ⅣႺቇળᐕળ㧔⑺↰Ꮢ㧕㧘ᐔᚑᐕ᦬ᣣ-ᣣ㧙ㆶర೷ᜂᜬᴺߦࠃࠆ NO ᵺൻᛛⴚ㧙㜞ᵴᕈ὇⚛❫⛽ࠍ↪޿ߚᴪ㆏ ਅේቁ┨ⅣႺᡷༀ⺞ᩏ⎇ⓥᚑᨐ⊒⴫ળ㧔ᣂឃࠟࠬ೥ᷫᛛⴚߦ㑐ߔࠆ⺞ᩏኋ඙㧕㧘ᐔᚑᐕ᦬ᣣ 

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㜞ᵴᕈ὇⚛❫⛽ࠍ↪޿ߚⅣႺ ᧼၂ᚑᵏ㧘ജ⴫⠪ቇળฬ㧔႐ᚲ㧕㧘ᐕ᦬ᣣኼ㓶㧘ᣂ⼱ବੑ㧘ਅේቁ┨㧘╙࿁ᄢ᳇ⅣႺቇળᐕળ㧔⑺↰ᄢ᳇ᵺൻߦ㑐ߔࠆ⎇ⓥ㧔 10㧕 㜞ᯅᵗሶᏒ㧕㧘ᐔᚑᐕ᦬ᣣ-ᣣ㧙࿾ਅ㚢ゞ႐ߦ߅ߌࠆ01㨤ᵺൻߩߚ߼ߩታ⸽ൻ੍஻⹜㛎㧙᦭᣿ᶏߦᵹ౉ߔࠆ╳ᓟᎹ෸߮ ↰ਛ⟵ੱ㧘ᾢ⼱ඳผ㧘᧻የ⍫ㇱᎹߩ㒠㔎ᤨ⽶⩄㊂⺞ᩏ₹੗ᵗሶ㧘਄↰ብ㧘ਛ᧛෶ༀ㧘╙࿁ᣣᧄ᳓ⅣႺቇળ㧔ජ⪲Ꮢ㧕ୃ㧘㊁ਛᱜᶈ㧖ᐔᚑᐕ᦬ᣣ-ᣣብ㧘ਛ᧛෶ༀ㧘ᐔ↰⟤↱♿㧖╙࿁ᣣᧄ᳓ⅣႺቇળ㧔ජ⪲Ꮢ㧕㧖 ⅣႺ଻ో⺖࿾ਅ᳓߳ߩ࡝ࡦ㉄ࠗࠝࡦߩ㜞 ⍹ᯅⲢሶ㧘᧻የỚᐲṁ಴ߦ㑐ߔࠆ⎇ⓥ

㧖 ਻Ꮊᄢቇ

ᐔᚑᐕ᦬ᣣ-ᣣඳᄙḧߦ߅ߌࠆࡑࠢࡠࡌࡦ࠻ ᾢ⼱ඳผ㧘ጊፒᗅ⟵㧖㧘ᷰㄝ੫৻㧖㧘⮮↰ஜ৻㧖╙࿁ⅣႺᎿቇ⎇ⓥࡈࠜ࡯࡜ࡓ

ࠬࠍ⠨ᘦߒߚ᳓⾰⸃ᨆ㧔ችፒᏒ㧕㧘㧖 ⑔ጟᄢቇ㧖 ਻ᎺⅣႺ▤ℂදળ

ᐔᚑᐕ᦬ᣣ-ᣣၒ┙ᑄ᫈‛ߩℂൻቇ⊛ᕈ⁁ߣ 㜞ᯅᶈม㧘ၳᎹ๺⟤㧘਎⦟ᥰਯ㧘ựፒశብ㧘⍹㤥 ╙࿁ᑄ᫈‛ቇળ⎇ⓥ⊒⴫ળ㧔㜞ᓸ↢‛ฌߣߩ㑐ଥ㕏ዏ㧘࿯↰ᄢテ㧘㠽⠀ፄ᮸㧘᳗ἑ⺈㧘ቝㇺችᓃ ᧻Ꮢ㧕㧘ᐔᚑᐕ᦬ᣣ

὾ළἯࠍේᢱߦ↪޿ߚ࡟ࡦࠟ 㜞ᯅᶈม㧘࿯↰ᄢテ㧘㠽⠀ፄ᮸㧘᳗ἑ⺈㧘ቝㇺ ╙࿁ᑄ᫈‛ቇળ⎇ⓥ⊒⴫ળ㧔㜞ߩ⵾ㅧᤨߦ߅ߌࠆ฽᦭ᚑಽߩ ችᓃ㧘ዊ᧻ᄞሶ 㧘ਛ㊁ㄖඳ 㧘↰ਛ

ᶈ㧖

㧖

᜼േ

㧖

㧖 ⑔ጟ⋵Ꮏᬺᛛⴚ࠮ࡦ࠲࡯

቟ቯဳ↥ᬺᑄ᫈‛ಣಽ႐ߩ᳓ 㜞ᯅᶈม㧘࿯↰ᄢテ㧘㠽⠀ፄ᮸㧘᳗ἑ
㊂⺞ᩏ

ችᓃ

᧻Ꮢ㧕
㧘ᐔᚑᐕ᦬ᣣ㧖 ⨹ᧁ┇ᬺ⺈㧘ቝㇺ ో࿖ⅣႺ⎇ද⼏ળડ↹ㇱળ ᑄ᫈‛ዊᆔຬળᑄ᫈‛⎇ⓥ⊒⴫ળ㧔ජઍ↰඙㧕㧘ᐔᚑᐕ᦬ᣣ

ࡅࡔ࠼ࡠࡓࠪ⑼ߦ⷗ࠄࠇࠆ⍴ ✜ᣇ⠅ဳߣߘߩ↢ᕷⅣႺ

ஜ㧘ਛፉ

ᷕ㧖

㧖 ਻Ꮊᄢቇ

⑔ጟ⋵ਅߩᴡᎹḮᵹㇱߩᄢဳ ✜ᣇᣣᧄ㒽᳓ቇળ╙࿁ᄢળ㧔ᣂẟᏒ㧕㧘ᐔᚑᐕ᦬-ᣣஜ㧘ጊፒᱜᢅ

╙࿁ో࿖ⅣႺ⎇ⓥᚲ੤ᵹࠪࡦ

ᐩ↢േ‛⋧㧙㉄ᕈ㔎᫪ᨋ↢ᘒࡐࠫ࠙ࡓ㧔ߟߊ߫Ꮢ㧕㧘♽ᓇ㗀⺞ᩏࠃࠅ㧙ᐔᚑᐕ᦬ᣣ

Ꮢ᳃ෳടߦࠃࠆḨේࡕ࠾࠲࡝ 㗇↰㓉৻╙࿁⥄ὼ♽⺞ᩏ⎇ⓥᯏ㑐ㅪ⛊ળࡦࠣ⺞ᩏ㧙ᐔየบᐢ⼱Ḩේߩ⼏㧔ን჻ศ↰Ꮢ㧕㧘੐଀㧙ᐔᚑᐕ᦬ᣣ

⸘㧔࿖ౝቇળ㧔ో࿖㧕㧕 ઙ 

Ԛ࿖ౝቇળ㧔࿾ᣇ㧕Ṷ㗴ฬᴤ∝ᬌ⸻ฃ⸻⠪࠺࡯࠲ࡌ࡯ࠬߩ᭴▽
ߦߟ޿ߡ

⊒

⴫

⠪

ቇળฬ㧔႐ᚲ㧕㧘ᐕ᦬ᣣጟᕶ৻㇢㧘↲ේ㓉⍫㧘㜞ጊᶈ᮸㧘ዊ ╙࿁਻Ꮊⴡ↢ⅣႺᛛⴚද⼏ળ㧔ᾢ
㊁Ⴆᄢ੺㧘㘵↰㓉㓶㧘ศ᧛ஜᷡ㧘਄ࡁ ᧄᏒ㧕㧘ᐔᚑᐕ᦬ᣣ
࿯ᱞ㧘ฎᳯჇ㓉

EAST↥↢ᄢ⣺⩶ࠍේ࿃ߣߔࠆ㘩ਛ ựፒశብ㧘᧛਄శ৻㧘ၳᎹ๺⟤㧘㐳㊁ ਻Ꮊⴡ↢ⅣႺᛛⴚද⼏ળ㧔ᾢᧄᏒ㧕㧘Ქ੐଀⧷ବ㧖㧘⍹㤥㕏ዏᐔᚑᐕ᦬ᣣ㧖 ↰Ꮉ଻ஜ⑔␩ⅣႺ੐ോᚲᶏ㕙ၒ┙ߡಣಽ႐࿯ფ෸߮ᶐ಴᳓ਛ ၳᎹ๺⟤㧘ựፒశብ㧘㜞ᯅᶈม㧘਎⦟ ╙࿁ᣣᧄ⚦⩶ቇળ਻Ꮊᡰㇱ✚ળߩ⎫㉄ㆶర⩶

ᥰਯ㧘⍹㤤㕏ዏ㧘ቝㇺችᓃ㧘⼱ญೋ⟤㧖 㧔⑔ጟᏒ㧕㧘ᐔᚑᐕ᦬ᣣ㧖 ↥ᬺක⑼ᄢቇකቇㇱ

eaeA ㆮવሶࠍᬌ಴ߒߚᄢ⣺⩶ Oߦ ᧛਄శ৻㧘㐳㊁⧷ବ 㧖 㧘ựፒశብ㧘ၳ ╙࿁⑔ጟ⋵౏ⴐⴡ↢ቇળ㧔⑔ጟࠃࠆ㘩ਛᲥ੐଀

Ꮉ๺⟤㧘⍹㤤㕏ዏ㧘㜞↰ೣሶ㧖 㧘᪢ፒ Ꮢ㧕㧘ᐔᚑᐕ᦬ᣣ⺈ᴦ㧖㧖 ↰Ꮉ଻ஜ⑔␩ⅣႺ੐ോᚲ㧖 ↢ᵴⴡ↢⺖↲⁁⣼ࠍ฽᦭ߔࠆஜᐽ㘩ຠਛߩ' ᫪↰㇌ᱜ㧘Ძ೑㓉⟤㧘ਛᎹ␞ሶ╙࿁਻Ꮊⴡ↢ⅣႺᛛⴚද⼏ળ㧔ᾢ -࠻࡝࡛࡯࠼࠴ࡠ࠾ࡦ෸߮࠴ࡠࠠࠪ

ᧄᏒ㧕㧘ᐔᚑᐕ᦬ᣣ

ࡦߩ HPLC ಽᨆᴺஜᐽ㘩ຠਛߩ࡝ࠝ࠴ࡠ࠾ࡦ࠽࠻࡝࠙ ᫪↰㇌ᱜ㧘Ძ೑㓉⟤㧘ਛᎹ␞ሶ╙࿁਻Ꮊⴡ↢ⅣႺᛛⴚද⼏ળ㧔ᾢࡓ෸߮࡟ࡏ࠴ࡠࠠࠪࡦ࠽࠻࡝࠙ࡓߩ

ᧄᏒ㧕㧘ᐔᚑᐕ᦬ᣣ

HPLC ಽᨆᴺ
ࠞࠥࡠ࠙ߩಽᏓ⁁ᴫ߆ࠄ⷗ߚ⑔ጟ⋵ ✜ᣇஜ㧘ጊፒᱜᢅ

ਅߩᴡᎹ╙࿁਻Ꮊⴡ↢ⅣႺᛛⴚද⼏ળ ᾢᧄᏒ㧕㧘ᐔᚑᐕ᦬-ᣣർ਻ᎺᏒߦ߅ߌࠆ⛘Ṍෂᗋ⒳ࠟࠪࡖ ⌀㍿
ࡕࠢߩ↢⢒⁁ᴫߣ↢⢒ⅣႺᔀ㧖㧘㗇↰㓉৻㧘ᄢ㊁⌬ሶ㧖㧘╣ ᣣᧄ↢ᘒቇળ਻Ꮊ࿾඙╙࿁ᄢળ

የᢕሶ

㧔⑔ጟᏒ㧕
㧘ᐔᚑᐕ᦬ᣣ㧖 ർ਻ᎺᏒ┙⥄ὼผ࡮ᱧผඳ‛㙚㧖 ᳓⨲⎇ⓥળ⛘Ṍෂᗋᬀ‛ࠟࠪࡖࡕࠢߩ↢⢒⁁ᴫ 㗇↰㓉৻㧘⌀㍿
ߣ↢⢒ⅣႺᔀ㧖㧘ᄢ㊁⌬ሶ㧖㧘╣ ╙࿁ࠕࠢࠕ⎇ⓥ࠮ࡦ࠲࡯⎇ⓥ⊒⴫የᢕሶળ㧔ർ਻ᎺᏒ㧕㧘ᐔᚑᐕ᦬ᣣ㧖 ർ਻ᎺᏒ┙⥄ὼผ࡮ᱧผඳ‛㙚㧖 ᳓⨲⎇ⓥળ⸘㧔࿖ౝቇળ㧔࿾ᣇ㧕㧕 ઙ 

㧔㧠㧕ႎ๔ᦠ৻ⷩ
ᆔ⸤੐ᬺฬ

ႎ

๔

ᦠ

ฬ

ᐔᚑᐕᐲෘ↢ഭ௛⑼ቇ⎇ⓥ 㧔ಽᜂ⎇ⓥႎ๔ᦠ㧕⾌⵬ഥ㊄ၫศ᧛ஜᷡ㧘㊄ሶ㘩ຠߩ቟ోᕈ㜞ᐲ ᾲᇦ૕ߩੱ૕ᓇ㗀ߣߘߩᴦ≮ᴺ╬ߦൻផㅴ⎇ⓥ੐ᬺ

㑐ߔࠆ⎇ⓥ㧔✚᜝㧕⠪⊒ⴕᐕ᦬⡡㧖㧘㜞ጊᶈ᮸㧘 ᐔᚑᐕ᦬

ጟᕶ৻㇢㧘ዊ㊁Ⴆᄢ੺㧖 ࿖┙߇ࠎ࠮ࡦ࠲࡯

ᐔᚑᐕᐲෘ↢ഭ௛⑼ቇ⎇ⓥ 㧔ಽᜂ⎇ⓥႎ๔ᦠ㧕⾌⵬ഥ㊄╩ጟᕶ৻㇢㧘ዊ㊁Ⴆᄢ੺㧘㜟የ ᐔᚑᐕ᦬

㘩ຠߩ቟ోᕈ㜞ᐲ ᾲᇦ૕ߩੱ૕ᓇ㗀ߣߘߩᴦ≮ᴺ╬ߦ ૫ሶ㧘㜞ጊᶈ᮸ൻផㅴ⎇ⓥ੐ᬺ

㑐ߔࠆ⎇ⓥ㧙ᴤ∝৻ᢧᬌ⸻ߩో࿖

㓸⸘⚿ᨐ෸߮ᴤ∝ᖚ⠪࠺࡯࠲ࡌ࡯ࠬ
ߩ᭴▽㧙ⅣႺ⋭ᆔ⸤ᬺോ

ᐔᚑᐕᐲൻቇ‛⾰ⅣႺታᘒ⺞ᩏ ർ㧔ೋᦼⅣႺ⺞ᩏ㧕ႎ๔ᦠ⋥ሶ㧘቟ᱞᄢテ㧘㘧⍹๺ᄢ㧘 ᐔᚑᐕ᦬

ᄢ㊁ஜᴦ㧘᧻ᨑ㓉ᒾ㧘᪉ᧁᑪᴦ㧘ᩉᎹᱜ↵㧘ጤᧄ⋿ੑ㧘੗਄ዅⴕ㧖㧘ਅựᱜᛚ㧖㧖 ⅣႺ଻ో⺖ᐔᚑᐕᐲൻቇ‛⾰ⅣႺታᘒ⺞ᩏ ർ㧔᥸㔺㊂⺞ᩏ㧕ႎ๔ᦠ⋥ሶ㧘቟ᱞᄢテ㧘㘧⍹๺ᄢ㧘 ᐔᚑᐕ᦬

ᄢ㊁ஜᴦ㧘᧻ᨑ㓉ᒾ㧘᪉ᧁᑪᴦ㧘੗਄ዅⴕ㧖㧘ਅựᱜᛚ㧖㧖 ⅣႺ଻ో⺖ᐔᚑᐕᐲൻቇ‛⾰ⅣႺታᘒ⺞ᩏ ർ㧔ࡕ࠾࠲࡝ࡦࠣ⺞ᩏ㧕ႎ๔ᦠ⋥ሶ㧘቟ᱞᄢテ㧘㘧⍹๺ᄢ㧘 ᐔᚑᐕ᦬

ᄢ㊁ஜᴦ㧘᧻ᨑ㓉ᒾ㧘᪉ᧁᑪᴦ㧘੗਄ዅⴕ㧖㧘ਅựᱜᛚ㧖㧖 ⅣႺ଻ో⺖ෘ↢ഭ௛⑼ቇ⎇ⓥ⵬ഥ㊄

᷷ᴰ࡮౏ⴐᶎ႐㧘ߘߩઁߩ᷷᳓ⅣႺ ㆙⮮ථ㇢㧖㧔ਥછ⎇ⓥ⠪㧕߇ࠎ੍㒐╬ஜᐽ⑼ቇ✚ว⎇ⓥ ߦ߅ߌࠆࠕࡔ࡯ࡃᕈ㜑⤑⣖Ἳߩ∛ේ ᧛਄శ৻੐ᬺઁ

૕ 0CGINGTKC HQYNGTK ߩ∉ቇߣ∛ 㧖 ࿖┙ᗵᨴ∝⎇ⓥᚲේᕈ⊒⃻ߦ㑐ߔࠆ⎇ⓥ

ႎ๔ᦠᐔᚑᐕᐲᑄ᫈‛ಣℂ╬⑼ቇ 㧔✚᜝࡮ಽᜂ⎇ⓥႎ๔ᦠ㧕⎇ⓥ੐ᬺ

ᐔᚑᐕ᦬

⼱ญೋ⟤㧖㧔ઍ⴫⎇ⓥ⠪㧕ᐔᚑᐕ᦬

ᑄ᫈‛ಣಽ႐ߩࡃࠗࠝ⹏ଔߦ㑐ߔࠆ 㜞ᯅᶈม㧘࿯↰ᄢテ㧘㠽⠀ፄ᮸㧘
⎇ⓥ㧔ᑄ᫈‛ಣಽ႐╬ߩ࿯ფߩℂൻ ᳗ἑቇ⹜㛎㧕⺈㧘ቝㇺችᓃ㧘⍹㤥㕏ዏ㧘ၳᎹ๺⟤㧘਎⦟ᥰਯ㧘ựፒశብ㧖 ↥ᬺක⑼ᄢቇ

㧔✚᜝࡮ಽᜂ⎇ⓥႎ๔ᦠ㧕⼱ญೋ⟤㧖㧔ઍ⴫⎇ⓥ⠪㧕ᑄ᫈‛ಣಽ႐ߩࡃࠗࠝ⹏ଔߦ㑐ߔ ၳᎹ๺⟤㧘 ựፒశብ㧘਎⦟ᥰਯ㧘ࠆ⎇ⓥ㧔⎫㉄ㆶర⩶ߩၭ㙃㧕㜞ᯅᶈม㧘⍹㤥㕏ዏ㧘ቝㇺችᓃ㧖 ↥ᬺක⑼ᄢቇ

 

ᐔᚑᐕ᦬

ᆔ⸤੐ᬺฬႎ๔ᦠฬᐔᚑᐕᐲᑄ᫈‛ಣℂ╬⑼ቇ 㧔✚᜝࡮ಽᜂ⎇ⓥႎ๔ᦠ㧕
⎇ⓥ੐ᬺ

ၫ

╩

⠪

⊒ⴕᐕ᦬

⼱ญೋ⟤㧖㧔ઍ⴫⎇ⓥ⠪㧕ᐔᚑᐕ᦬

ᑄ᫈‛ಣಽ႐ߩࡃࠗࠝ⹏ଔߦ㑐ߔࠆ ਎⦟ᥰਯ㧘㜞ᯅᶈม㧘ựፒశብ㧘⎇ⓥ㧔ࡔ࠲ࡦ↢ᚑ⩶ߩၭ㙃㧕ၳᎹ๺⟤㧘ජ‫ޘ‬๺ൎᏆ㧘⍹㤥㕏ዏ㧘᳗ἑ⺈㧘ቝㇺችᓃ㧖 ↥ᬺක⑼ᄢቇ
㧔✚᜝࡮ಽᜂ⎇ⓥႎ๔ᦠ㧕⼱ญೋ⟤㧖㧔ઍ⴫⎇ⓥ⠪㧕ᐔᚑᐕ᦬

ᑄ᫈‛ಣಽ႐ߩࡃࠗࠝ⹏ଔߦ㑐ߔࠆ ựፒశብ㧘ၳᎹ๺⟤㧘਎⦟ᥰਯ㧘⎇ⓥ ህ᳇ᕈ⚦⩶ߩSrDNA Ⴎၮ㈩ 㜞ᯅᶈม㧘⍹㤥㕏ዏ㧘ቝㇺችᓃ೉᳿ቯߦࠃࠆᓸ↢‛⟲㓸⸃ᨆ㧕ᐔᚑᐕᐲෘ↢⑼ቇ⎇ⓥ⾌⵬ 㧔✚᜝࡮ಽᜂ⎇ⓥႎ๔ᦠ㧕ഥ㊄㧖 ↥ᬺක⑼ᄢቇ
ዊᾢᗆੑ* 㧔ਥછ⎇ⓥ⠪㧕㧘ᐔᚑᐕ᦬

㘩ຠߩ቟ోᕈ㜞ᐲൻផ ኈེ൮ⵝ⹣ૐ㉄ᕈ㘩ຠߩࡏ࠷࡝࠿ࠬ ၳᎹ๺⟤㧘ựፒశብ㧘᧛਄శ৻㧘ㅴ⎇ⓥ੐ᬺ㘩ਛᲥߦኻߔࠆ࡝ࠬࠢ⹏ଔ

㊁↰ᄙ⟤ᨑ㧘⍹㤥㕏ዏઁ㧖 ጟጊᄢቇ

ᐔᚑ -ᐕᐲෘ↢⑼ቇ⎇ⓥ 㧔✚᜝࡮ಽᜂ⎇ⓥႎ๔ᦠ㧕⾌⵬ഥ㊄ዊᾢᗆੑ* 㧔ਥછ⎇ⓥ⠪㧕㧘ᐔᚑᐕ᦬

㘩ຠߩ቟ోᕈ㜞ᐲ ኈེ൮ⵝ⹣ૐ㉄ᕈ㘩ຠߩࡏ࠷࡝࠿ࠬ ၳᎹ๺⟤㧘ựፒశብ㧘᧛਄శ৻㧘ൻផㅴ⎇ⓥ੐ᬺ

㘩ਛᲥߦኻߔࠆ࡝ࠬࠢ⹏ଔ

㊁↰ᄙ⟤ᨑ㧘┻ਛ㊀ᐘ㧚⍹㤥㕏ዏઁ㧖 ጟጊᄢቇ

ᐔᚑᐕᐲ⑔ጟ⋵࡝ࠨࠗࠢ࡞ ‫ޟ‬ⅣႺߦఝߒ޿⚕߅߻ߟ㐿⊒⎇ⓥ‫ ޠ‬⍹ᯅື਽㧘ᳯ⮮⦟᮸㧘਎⦟ᥰਯ㧘 ᐔᚑᐕ᦬
✚ว⎇ⓥ࠮ࡦ࠲࡯⎇ⓥળႎ๔ᦠජ‫ޘ‬๺ൎᏆ㧘᧛਄శ৻㧘㊁↰ᄙ⟤ᨑ㧘ựፒశብ㧘┻ਛ㊀ᐘ㧘ၳᎹ๺⟤㧘⍹㤥㕏ዏᐔᚑᐕᐲෘ↢ഭ௛⑼ቇ⎇ⓥ㧔ᐔᚑᐕᐲ✚᜝࡮ಽᜂ⎇ⓥႎ๔ᦠ㧕ਛᎹ␞ሶ㧘⧃Ⴆ↱♿㧘ၳ⾌⵬ഥ㊄੐ᬺዞ⧷㧘 ᐔᚑᐕ᦬

㘩ຠ቟ో⏕଻⎇ⓥ 㘩ຠਛ⥇⚛ൻ࠳ࠗࠝࠠࠪࡦ෸߮ߘߩ 㘧⍹๺ᄢ㧘㘵↰㓉㓶㑐ㅪൻว‛⾰ߩᳪᨴታᘒߩ⸃᣿ߦ㑐ߔࠆ⎇ⓥ㧔ᐔᚑ -ᐕᐲ✚ว⎇ⓥႎ๔ᦠ㧕 ਛᎹ␞ሶ㧘⧃Ⴆ↱♿㧘ၳዞ⧷㧘 ᐔᚑᐕ᦬

㘩ຠਛ⥇⚛ൻ࠳ࠗࠝࠠࠪࡦ෸߮ߘߩ 㘧⍹๺ᄢ㧘㘵↰㓉㓶㑐ㅪൻว‛⾰ߩᳪᨴታᘒߩ⸃᣿ߦ㑐ߔࠆ⎇ⓥ
㧔⎇ⓥႎ๔ᦠ㧕૒‫ᧁޘ‬ਭ⟤ሶ 㧖㧘☨⼱᳃㓶 㧖 㧘㘵 ᐔᚑᐕ᦬

࠳ࠗࠝࠠࠪࡦߩᳪᨴታᘒᛠី෸߮៨ ↰㓉㓶㧘ႇขૐᷫൻߦ㑐ߔࠆ⎇ⓥ

 

ᥓᤘ㧖㧘ᄤୖศ┨㧖㧖 ࿖┙ක⮎ຠ㘩ຠⴡ↢⎇ⓥᚲ

ᆔ⸤੐ᬺฬ

ႎ

๔

ᦠ

ฬ

ၫ

ᐔᚑᐕᐲෘ↢ഭ௛⑼ቇ⎇ⓥ 㧔ᐔᚑ-ᐕᐲ✚ว⎇ⓥႎ๔ᦠ㧕⾌⵬ഥ㊄ขૐᷫൻߦ㑐ߔࠆ⎇ⓥ

⠪

⊒ⴕᐕ᦬

⼾↰ᱜᱞ 㧖 㧘૒‫ᧁޘ‬ਭ⟤ሶ 㧖 㧘☨ ᐔᚑᐕ᦬

㘩ຠ቟ో⏕଻⎇ⓥ ࠳ࠗࠝࠠࠪࡦߩᳪᨴታᘒᛠី෸߮៨ ⼱᳃㓶㧖㧘ႇ੐ᬺ╩ᥓᤘ㧖㧘ᄤୖศ┨㧖㧘㘵↰㓉㓶㧖 ࿖┙ක⮎ຠ㘩ຠⴡ↢⎇ⓥᚲ

㧔ᾲᇦ૕ߦ㑐ߔࠆ⎇ⓥ࡮ಽᜂ⎇ⓥႎ 㘵↰㓉㓶㧘ਛᎹ␞ሶ㧘⧃Ⴆ↱♿㧘 ᐔᚑᐕ᦬
๔ᦠ㧕

ၳ

ዞ⧷㧘

ጟᕶ৻㇢

ᴤ∝ᖚ⠪ⴊਛ PCB ╬ㅊ〔⺞ᩏߦ߅ߌࠆಽᨆᴺߩᡷ⦟߅ࠃ߮ߘߩ⹏ଔߦ
㑐ߔࠆ⎇ⓥᐔᚑᐕᐲෘ↢ഭ௛⑼ቇ⎇ⓥ 㧔ᾲᇦ૕ߩੱ૕ᓇ㗀ߣߘߩᴦ≮ᴺߦ 㘵↰㓉㓶㧘ਛᎹ␞ሶ㧘⾌⵬ഥ㊄㘩ຠߩ቟ోᕈ㜞ᐲ 㑐ߔࠆ⎇ⓥ

ൻផㅴ⎇ⓥ੐ᬺጟᕶ৻ ᐔᚑᐕ᦬

ᐔᚑᐕᐲ✚᜝࡮ಽᜂ ㇢㧘᧻ᨑ㓉ᒾ㧘ᐔᎹඳ઄㧘ၳ⎇ⓥႎ๔ᦠ㧕

ዞ⧷㧘㘧⍹๺ᄢ㧘⧃Ⴆ↱♿㧘ዊᴤ∝ᖚ⠪߅ࠃ߮ஜᏱੱ૕ౝߩ PCDF ㊁Ⴆᄢ੺㧘ᚭ㜞
㘃ታᘒ⺞ᩏ

ዅ㧖

㧖 ᣣᧄ㘩ຠⴡ↢දળ㧔ᾲᇦ૕ߩੱ૕ᓇ㗀ߣߘߩᴦ≮ᴺߦ 㘵↰㓉㓶㧘ਛᎹ␞ሶ㧘㑐ߔࠆ⎇ⓥ

ጟᕶ৻ ᐔᚑᐕ᦬

ᐔᚑᐕᐲ✚᜝࡮ಽᜂ ㇢㧘᧻ᨑ㓉ᒾ㧘ᐔᎹඳ઄㧘ၳ⎇ⓥႎ๔ᦠ㧕

ዞ⧷㧘㘧⍹๺ᄢ㧘⧃Ⴆ↱♿㧘ዊࠥ࡞ᶐㅘࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯㧔GPC㧕 ㊁Ⴆᄢ੺㧘ᚭ㜞ዅ㧖෸߮㜞ಽ⸃⢻ࠟࠬࠢࡠࡑ࠻ࠣ࡜ࡈࠖ 㧖 ᣣᧄ㘩ຠⴡ↢දળ࡯ /㜞 ಽ ⸃ ⢻ ⾰ ㊂ ಽ ᨆ ᴺ
㧔HRGC/HRMS㧕ߦࠃࠆᴤ∝ᖚ⠪ߩⴊਛ PCB หᣖ૕ಽ㔌ಽᨆᐔᚑᐕᐲෘ↢ഭ௛⑼ቇ⎇ⓥ 㧔೨ะ߈ࠦࡎ࡯࠻⎇ⓥߦࠃࠆవᄤ⇣ 㘵↰㓉㓶㧘ਛᎹ␞ሶ㧘᧻ᨑ㓉ᒾ㧘 ᐔᚑᐕ᦬
⾌⵬ഥ㊄ஜᐽ቟ో⏕଻✚ว Ᏹࡕ࠾࠲࡝ࡦࠣ㧘․ߦዩ㆏ਅⵚ㧘஗ ᐔᎹඳ઄㧘ၳዞ⧷㧘㘧⍹๺ᄢ㧘⎇ⓥ ൻቇ‛⾰࡝ࠬࠢ⎇ⓥ੐ ⇐♖Ꮍߩ࡝ࠬࠢⷐ࿃ߣౝಽᴲ߆ߊੂ ⧃Ⴆ↱♿㧘ᚭ㜞ᬺ‛⾰ߦኻߔࠆᗵฃᕈߩ⸃᣿ዅ㧖ಽᜂ⎇ 㧖 ᣣᧄ㘩ຠⴡ↢දળⓥႎ๔ᦠ㧕ᅧᇚ㧔ੱ㧕ߩⴊਛ PCDDs㧘PCDFs㧘
ࡁࡦࠝ࡞࠰ Co-PCBs ߅ࠃ߮ࡕࡁࠝ
࡞࠰ Co-PCBs Ớᐲ㧔-ᐕ㧕㧔ౝಽᴲ߆ߊੂ‛⾰࡮࠳ࠗࠝࠠࠪࡦ ጊ↰ஜੱ 㧖 㧘㘵↰㓉㓶㧘 ᚭ㜞㘃ߩዊఽ㧘ᚑੱߩᳪᨴታᘒ෸߮᥸㔺 ዅ 㧖  㧘ᐔᎹඳ઄㧘ਛᎹ␞ሶ㧘᧻ߦ㑐ߔࠆ⺞ᩏ⎇ⓥᐔᚑᐕ -ᐕ ᨑ㓉ᒾ㧘ၳ✚วಽᜂ⎇ⓥႎ๔ᦠ㧕ዞ⧷㧘㘧⍹๺ᄢ㧖 ᘮᙥ⟵Ⴖᄢቇ㧖 ᣣᧄ㘩ຠⴡ↢දળ 

ᐔᚑᐕ᦬

ᆔ⸤੐ᬺฬ

ႎ

๔

ᦠ

ฬ

ၫ

╩

ᐔᚑᐕᐲෘ↢ഭ௛⑼ቇ⎇ⓥ 㧔ౝಽᴲ߆ߊੂ‛⾰࡮࠳ࠗࠝࠠࠪࡦ 㘵↰㓉㓶㧘ᚭ㜞⾌⵬ഥ㊄⠪ዅ㧖㧘ᐔᎹඳ઄㧘 ᐔᚑᐕ᦬

ஜᐽ቟ో⏕଻✚ว 㘃ߩዊఽ㧘ᚑੱߩᳪᨴታᘒ෸߮᥸㔺 ਛᎹ␞ሶ㧘᧻ᨑ㓉ᒾ㧘ၳ⎇ⓥ ൻቇ‛⾰࡝ࠬࠢ⎇ⓥ੐ ߦ㑐ߔࠆ⺞ᩏ⎇ⓥ
ᬺ

⊒ⴕᐕ᦬

ዞ⧷㧘

ᐔᚑᐕᐲಽᜂ 㘧⍹๺ᄢ⎇ⓥႎ๔ᦠ㧕㧖 ᣣᧄ㘩ຠⴡ↢දળࡅ࠻೬ᬌ⹜ᢱਛߩ࠳ࠗࠝࠠࠪࡦ㘃㧔 PCDD/DFs㧘 Non-ortho-PCBs㧘Mono-ortho-PCBs㧕⺞ᩏᐔᚑᐕᐲෘ↢ഭ௛⋭ᆔབྷ੐ ක≮↪ක⮎ຠߩຠ⾰ౣ⹏ଔߦଥࠆ౏ Ძ೑㓉⟤㧘᫪↰㇌ᱜ㧘ਛᎹ␞ሶ㧘 ᐔᚑᐕ᦬
ᬺ⊛ṁ಴⹜㛎㧔᩺㧕ߩᅷᒰᕈᬌ⸽╬ႎ 㘵↰㓉㓶๔ᦠ㧔ᐔᚑᐕᐲ㧕ᐕ ~ᐕᣣ㖧ᶏጽᴪጯ ᣣ㖧ㇺᏒ㑆ᄢ᳇ᳪᨴᐲᲧセ⹏ଔႎ๔ ᣣ㖧ᶏጽᴪጯ⋵Ꮢ㆏ⅣႺᛛⴚ੤ ᐔᚑᐕ᦬
⋵Ꮢ㆏ⅣႺᛛⴚ੤ᵹ੐ᬺ

ᦠᵹળ⼏㧔ᄢ⍹⥝ᒄ㧕ᐕ ~ᐕᣣ㖧ᶏጽᴪጯ ਻Ꮊർㇱ㧟⋵෸߮ጊญ⋵ߦ߅ߌࠆᶋ ᣣ㖧ᶏጽᴪጯ⋵Ꮢ㆏ⅣႺᛛⴚ੤ ᐔᚑᐕ᦬
⋵Ꮢ㆏ⅣႺᛛⴚ੤ᵹ੐ᬺ

ㆆ☸ሶ⁁‛⾰ᚑಽ⺞ᩏႎ๔ᦠᵹળ⼏㧔ᄢ⍹⥝ᒄ㧕ᐔᚑᐕᐲⅣႺ᡼኿⢻᳓Ḱ੐ ╙㧠㧢࿁ⅣႺ᡼኿⢻⺞ᩏ⎇ⓥᚑᨐ⺰ ᢥㇱ⑼ቇ⋭㧔↰਄྾㇢㧘ᰞ੗೑ ᐔᚑᐕ᦬
ᬺ

ᢥᛞ㍳㓸㧔ᐔᚑᐕᐲ㧕ᒾ㧘ጤᧄ⋿ੑ㧕ᐕᐲ౏ኂஜᐽⵍኂ⵬ఘ੍ 㜞ᵴᕈ὇⚛❫⛽ࠍ↪޿ߚᴪ㆏ឃࠟࠬ ⑔ጟ⋵㧔ਅේቁ┨㧕㒐දળ೥ᷫᛛⴚߦ㑐ߔࠆ⺞ᩏႎ๔ᦠᐔᚑᐕᐲෘ↢ഭ௛⑼ቇ⎇ⓥ ో࿖ⷙᮨߩ∉ቇ⎇ⓥߦࠃࠆࠪ࠶ࠢࡂ ജ⾌⵬ഥ㊄ᐔᚑᐕ᦬

ኼ㓶㧘ጤᧄ⋿ੑ㧘ศ᧛ஜᷡᐔᚑᐕ᦬

ኼ㓶㧘ጤᧄ⋿ੑ㧘ศ᧛ஜᷡᐔᚑᐕ᦬

ஜᐽ⑼ቇ✚ว⎇ⓥ ࠙ࠬߩታᘒߣේ࿃ߩ⸃᣿

੐ᬺർ਻Ꮊߦ߅ߌࠆࠪ࠶ࠢࡂ࠙ࠬ∝୥⟲ߩታᘒ⺞ᩏᐔᚑᐕᐲෘ↢ഭ௛⑼ቇ⎇ⓥ ቶౝⓨ᳇⾰ߩஜᐽᓇ㗀ߦଥࠆකቇ⊛ ജ⾌⵬ഥ㊄ෘ↢ഭ௛⑼ቇ․೎ ⍮⷗ߩᢛℂ⎇ⓥ੐ᬺࠪ࠶ࠢࡂ࠙ࠬ∝୥⟲ߩ⥄ⷡ∝⁁ߣⷐ࿃ߦߟ޿ߡ᦭᣿ᶏᵹၞᳪỘ⽶⩄ኻ╷⺞ᩏ ᦭᣿ᶏᵹၞߦ߅ߌࠆᳪỘ⽶⩄㊂ᛠី ↰ਛ⟵ੱ㧘ᾢ⼱ඳผ㧘᧻የ⺞ᩏႎ๔ᦠብ㧘 ᐔᚑᐕ᦬

ਛ᧛෶ༀ᦭᣿ᶏᵹၞᳪỘ⽶⩄ኻ╷⺞ᩏ ᦭᣿ᶏᵹၞ⽶⩄㊂ኻ╷⺞ᩏ㧦㒠㔎ᤨ ↰ਛ⟵ੱ㧘ᾢ⼱ඳผ㧘᧻የብ㧘⺞ᩏႎ๔ᦠ↢ᵴឃ᳓ኻ╷੐ᬺᐔᚑᐕ᦬

ਛ᧛෶ༀᒾጊᎹᵹၞ↢ᵴឃ᳓ኻ╷ផㅴ⸘↹⍹ᯅⲢሶ㧘ᬰፒᐘ▸㧘✜ᣇ㗇↰㓉৻ ஜ㧘 ᐔᚑᐕ᦬

ᆔ⸤੐ᬺฬ
ἑᚭౝᶏⅣႺᖱႎၮᧄ⺞ᩏ

ႎ

๔

ᦠ

ฬ

ၫ

╩

⠪

⊒ⴕᐕ᦬

ᐔᚑᐕᐲⅣႺ⋭ᆔ⸤ᬺോ⚿ᨐႎ๔ ᾢ⼱ඳผ㧘᳗ᷗ⟵ቁ㧘ਛ᧛෶ༀᦠἑᚭౝᶏⅣႺᖱႎၮᧄ⺞ᩏᐔᚑᐕ᦬

㧙ᄢ㒋ḧ࡮ᐢፉḧ࡮દ੍ἥ࡮㗀ἥ㧙㧔⾗ᢱ✬㧕ᐔᚑᐕᐲ⑔ጟ⋵࡝ࠨࠗࠢ࡞ ‫ޟ‬὾ළἯࡍ࡟࠶࠻ߩ⾗Ḯൻ⎇ⓥ‫ޠ‬ႎ ⍹ᯅⲢሶ㧘ᬰፒᐘ▸✚ว⎇ⓥ࠮ࡦ࠲࡯⎇ⓥળ

ᐔᚑᐕ᦬

๔ᦠᐔᚑᐕᐲ⑔ጟ⋵࡝ࠨࠗࠢ࡞ ‫ޟ‬ㇺᏒࠧࡒ὾ළἯࠍᵴ↪ߒߚࠛࠦ࡟ ቝㇺችᓃ㧘᳗ἑ⺈㧘㤥Ꮉ㓁৻㧘 ᐔᚑᐕ᦬

✚ว⎇ⓥ࠮ࡦ࠲࡯౒ห⎇ⓥࡊ ࡦࠟ⵾ㅧᛛⴚߩ㐿⊒ࡊࡠࠫࠚࠢ࠻⎇ 㠽⠀ፄ᮸㧘㜞ᯅᶈม㧘࿯↰ᄢテࡠࠫࠚࠢ࠻ⓥߦଥࠆ቟ోᕈ⹜㛎ᬺോ‫ޠ‬ႎ๔ᦠᐔᚑᐕᐲ⑔ጟ⋵࡝ࠨࠗࠢ࡞ ‫ޟ‬ⅣႺᜬ⛯ဳߩ὾ළᱷᩏᓴⅣ⾗Ḯൻ ᳗ἑ✚ว⎇ⓥ࠮ࡦ࠲࡯⎇ⓥળ

⎇ⓥ‫ޠ‬ႎ๔ᦠ

㜞ᯅᶈม㧘࿯↰ᄢテᐔᚑᐕᐲ⑔ጟ⋵࡝ࠨࠗࠢ࡞ ‫ޟ‬ⅣႺ౒↢ဳਅ᳓ᳪᵆಣℂ࡮೑↪ࠪ ᳗ἑ✚ว⎇ⓥ࠮ࡦ࠲࡯⎇ⓥળ⺈㧘㤥Ꮉ㓁৻㧘㠽⠀ፄ᮸㧘 ᐔᚑᐕ᦬

⺈㧘㤥Ꮉ㓁৻㧘㠽⠀ፄ᮸㧘 ᐔᚑᐕ᦬

ࠬ࠹ࡓߩ᭴▽ߦ㑐ߔࠆ⎇ⓥ ‫ޠ‬ႎ๔ 㜞ᯅᶈม㧘࿯↰ᄢテᦠⅣႺ⋭ᆔ⸤ᬺോ

ᐔᚑᐕᐲⅣႺ⋭ᆔ⸤ᬺോ⚿ᨐႎ๔ 㗇↰㓉৻㧘ጊፒᱜᢅᦠᐔᚑᐕ᦬

㉄ᕈ㔎ࡕ࠾࠲࡝ࡦࠣ㧔࿯ფ࡮ᬀ↢㧕⺞ᩏⅣႺ⋭ᆔ⸤ᬺോ

╙࿁⥄ὼⅣႺ଻ోၮ␆⺞ᩏ↢‛ 㗇↰㓉৻㧘✜ᣇᄙ᭽ᕈ⺞ᩏ⒳ߩᄙ᭽ᕈ⺞ᩏ㧔⑔ጟ⋵㧕ጊፒᱜᢅ㧘᧻የႎ๔ᦠਛፉஜ㧘ਛ᧛᦮ผ㧘 ᐔᚑᐕ᦬
ብ᧻੗⺈৻㧖㧘ᷕ 㧘㝩ୖᓼ㓶㧖㧘ቝㇺች㧖㕏჻㧖㧘ᧁේ⋥ੱ㧖㧘ศ↰㧖 ਻Ꮊᄢቇ㧖 ᣣᧄ㊁㠽ߩળ╳ᓟᡰㇱ⸘㧔ႎ๔ᦠ৻ⷩ㧕 

ઙഞ㧖

３

調査研究終了報告書⚳ੌ㧝⺞ᩏ⎇ⓥ⚳ੌႎ๔ᦠ
⎇ⓥಽ㊁㧦ᗵᨴ∝ߩ⊒↢᜛ᄢ㒐ᱛ෸߮㘩ຠߩ቟ోᕈ⏕଻ߦ㑐ߔࠆ⎇ⓥ
⺞

ᩏ

⎇

ⓥ

ฬ

࡟ࠫࠝࡀ࡜ኋਥࠕࡔ࡯ࡃߩ౏ⴐᶎ႐╬ߢߩಽᏓߣ↢ᘒߦ㑐ߔࠆ⎇ⓥ⎇ⓥ⠪ฬ㧔ᚲዻ㧕̪ ٤ශ㧦⎇ⓥઍ⴫⠪٤᧛਄శ৻㧘㊁↰ᄙ⟤ᨑ㧘ự㦮శብ㧘ၳᎹ๺⟤㧘⍹㤤㕏ዏ㧔∛ℂ⚦⩶⺖㧕㧘㆙⮮ථ㇢㧔࿖┙ᗵᨴ∝⎇ⓥᚲ㧕ᧄᐡ㑐ଥㇱ࡮⺖↢ᵴⴡ↢⺖⺞ ᩏ ⎇ ⓥ ᦼ 㑆ᐔᚑ㧝㧡ᐕᐲ⺞ ᩏ ⎇ ⓥ ⒳ ⋡㧝㧚‫ⴕع‬᡽⎇ⓥ‫⺖غ‬㗴⎇ⓥ‫౒غ‬ห⎇ⓥ㧔౒หᯏ㑐ฬ㧦‫غ‬ฃ⸤⎇ⓥ㧔ᆔ⸤ᯏ㑐ฬ㧦㧞㧚‫ع‬ၮ␆⎇ⓥ‫غ‬ᔕ↪⎇ⓥ㧟㧚‫غ‬㊀ὐ⎇ⓥ‫ع‬ផᅑ⎇ⓥ

㧙

㧝㧢ᐕᐲ

㧔㧞ᐕ㑆㧕
㧕
㧕
‫غ‬㐿⊒⎇ⓥ‫غ‬㧵㧿㧻ផㅴ⎇ⓥ߰ߊ߅߆ᣂ਎♿⸘↹╙㧞ᰴታᣉ⸘↹ᄢ㗄⋡㧦ሶߤ߽߿㜞㦂⠪ߣ↢߈↢߈᥵ࠄߔஜᐽ␠ળߠߊࠅ
ਛ㗄⋡㧦⋵᳃ߩஜᐽ⊛ߥ᥵ࠄߒߠߊࠅߩផㅴ
ዊ㗄⋡㧦∔∛࡮੍㒐ኻ╷ߩలታ (ᗵᨴ∝ኻ╷ߩផㅴ)⑔ጟ⋵ⅣႺ✚วၮᧄ⸘↹㧔㧼㧕̪ⅣႺ㑐ଥߩߺ

ᩇ 㧦
࠹࡯ࡑ㧦

ࠠ

Ԙࠕࡔ࡯ࡃ

࡯

ࡢ

࡯

࠼

ԙ࡟ࠫࠝࡀ࡜
⎇Ԛࠕࡔ࡯ࡃኻ╷
ⓥ

ߩ

᭎

ԛᶎ႐

Ԝⴡ↢▤ℂ

ⷐ

㧝㧕⺞ᩏ⎇ⓥߩ⋡⊛෸߮ᔅⷐᕈ
ㄭᐕ㧘౏ⴐᶎ႐㧔᥉ㅢᶎ႐㧘ࡋ࡞ࠬ࠮ࡦ࠲࡯㧘ࠬ࡯ࡄ࡯㌛ḡ߅ࠃ߮ࠨ࠙࠽㘑ํߥߤߩᣉ⸳㧕ߦ߅ߌࠆ㧘࡟ࠫࠝࡀ࡜
ᗵᨴ∝߇໧㗴ߣߥߞߡ޿ࠆ㧚࡟ࠫࠝࡀ࡜ߪ㧘ߎࠇࠄᶎ႐ߩ᷷ḡߩਛߢ㧘ࠕࡔ࡯ࡃ╬ࠍኋਥߣߒߡሽ࿷ߔࠆߣ⠨߃ࠄࠇߡ޿ࠆ㧚ߎߩߚ߼㧘ᧄ⎇ⓥߪ੹߹ߢ޽߹ࠅႎ๔ߩή޿ᶎ႐ߩࠕࡔ࡯ࡃߩಽᏓ߿↢ᘒߦߟ޿ߡ᣿ࠄ߆ߦߒᰴ޿ߢࠕࡔ࡯
ࡃࠍ೙ᓮߔࠆߎߣࠍ⋡⊛ߣߔࠆ㧚ᧄ⎇ⓥࠍផㅴߔࠆߎߣߪ࡟ࠫࠝࡀ࡜ߩ೙ᓮߦ߽ߟߥ߇ࠅ㧘ᔅⷐᕈ߇㜞޿ߣ⠨߃ࠄࠇࠆ㧚㧞㧕⺞ᩏ⎇ⓥߩ᭎ⷐ⋵ౝߩ౏ⴐᶎ႐ߩࠕࡔ࡯ࡃᳪᨴ⁁ᴫߦߟ޿ߡᛠីߔࠆߚ߼㧘౏ⴐᶎ႐ߩ⹜ᢱࠍᬌᩏߒߚ㧚ౕ૕⊛ߦߪ㧘ᶎ႐ߩ⸳஻ߩ㆑޿㧘᳓⾰ߩ㆑޿㧘▤ℂᣇᴺߩ㆑޿╬ࠍ⠨ᘦߒߡ⹜ᢱࠍណขߒ㧘ၭ㙃ᴺߦࠃࠅࠕࡔ࡯ࡃࠍᬌ಴ߒߚ㧚ᬌ಴ߒߚࠕ
ࡔ࡯ࡃߪㆮવሶᬌᩏߢ⒳ࠍหቯߒߚ㧚⸳஻㧘᳓⾰㧘޽ࠆ޿ߪ▤ℂᣇᴺߢࠕࡔ࡯ࡃߩᳪᨴ⁁ᴫߦ⋧㆑߇޽ࠆ߆ᬌ⸛ߒ
ߚ㧚หᤨߦ⹜ᢱߩ࡟ࠫࠝࡀ࡜ᬌᩏࠍታᣉߒ㧘ࠕࡔ࡯ࡃߣ࡟ࠫࠝࡀ࡜ߣߩ㑐ଥߦߟ޿ߡᬌ⸛ߒߚ㧚ᬌ⸛⚿ᨐࠍၮߦࠕࡔ
࡯ࡃߩ೙ᓮᣇᴺߦߟ޿ߡᮨ⚝ߔࠆߣߣ߽ߦ㧘ᶎ႐ߩ࡟ࠫࠝࡀ࡜ᳪᨴኻ╷ᣇᴺߦߟ޿ߡ߽ᮨ⚝ߒߚ㧚㧟㧕⺞ᩏ⎇ⓥߩ㆐ᚑᐲ෸߮ᓧࠄࠇߚᚑᨐ㧔ߢ߈ࠆߛߌᢙ୯ൻߒߡߊߛߐ޿㧕㆐ᚑᐲ㧦એ਄
ᓧࠄࠇߚᚑᨐ㧦⋵ౝᣉ⸳ߩࠕࡔ࡯ࡃᳪᨴ⁁ᴫ㧘࡟ࠫࠝࡀ࡜ᳪᨴ⁁ᴫ߇್᣿ߒߚ㧚ߎࠇࠄߩᳪᨴ߇⿠ߎࠆ⸳஻޽ࠆ޿ߪㆇ༡਄ߩ໧㗴ὐࠍᛠីߒߚ㧚㧠㧕⋵᳃ߩஜᐽߩ଻ᜬ෶ߪⅣႺߩ଻ో߳ߩ⽸₂ࠕࡔ࡯ࡃ㧘࡟ࠫࠝࡀ࡜ᳪᨴ߇޽ߞߚᣉ⸳ߩౝ㧘▎ᚲߦߟ޿ߡታ㓙ߦ⃻࿾ߦ⿞߈㧘଻ஜ⑔␩ⅣႺ੐ോᚲᜂᒰ⠪ߣߣ߽ߦኻ╷ߦߟ޿ߡ⠨߃㧘ឭ᩺ߒ㧘੐ᬺ⠪ߦታⴕߒߡ޿ߚߛ޿ߚ㧚ߎࠇߦࠃࠅ㧘ᒰ⹥੐ᬺ⠪ߪ߽ߣࠃࠅ㧘଻ஜ⑔␩ⅣႺ
੐ോᚲᜂᒰ⠪ߩ⷗⡞߽ᐢ߇ࠅ㧘㑆ធ⊛ߦ⋵᳃ߩஜᐽ଻ᜬߦᓎ┙ߞߚߣ⠨߃ࠄࠇࠆ㧚ߎߩ߶߆㧘଻ஜ⑔␩ⅣႺ੐ോᚲᬌᩏ⺖⡯ຬ߳ߩ⎇ୃࠍㅢߒߡ㧘ᛛⴚߣ⍮⼂ࠍવ㆐ߒ㧘ߎࠇߦࠃࠅ଻ஜ⑔␩ⅣႺ੐ോ
ᚲߦ߅ߌࠆᶎ႐ߩⴡ↢▤ℂ੐ᬺ߭޿ߡߪ⋵᳃ߩஜᐽ଻ᜬߦᓎ┙ߞߚ㧚㧡㧕⺞ᩏ⎇ⓥ⚿ᨐߩ⁛ഃᕈ㧘ᣂⷙᕈࠕࡔ࡯ࡃߩ⺞ᩏߪႎ๔ᢙ߇ዋߥߊ㧘ᣂⷙᕈ߇㜞޿㧚․ߦࠕࡔ࡯ࡃߩหቯߦࡐ࡝ࡔ࡜࡯࠯ㅪ㎮෻ᔕࠍ↪޿ߚㆮવሶ⸻
ᢿࠍ↪޿ࠆᛛⴚߪ㧘ᦨᣂߩ߽ߩߢ޽ࠆ㧚㧢㧕ᚑᨐߩᵴ↪⁁ᴫ㧔ᛛⴚ⒖ォ࡮ᵴ↪ߩน⢻ᕈ㧕଻ஜ⑔␩ⅣႺ੐ോᚲᬌᩏ⺖⡯ຬߩ⎇ୃᤨߦ㧘ࠕࡔ࡯ࡃߩಽ㔌ᴺ㧘࡟ࠫࠝࡀ࡜ߩᬌ಴ᴺߦߟ޿ߡᛛⴚ⒖ォߒߡ޿ࠆ㧚 

⚳ੌ㧞⺞ᩏ⎇ⓥ⚳ੌႎ๔ᦠ
⎇ⓥಽ㊁㧦࠳ࠗࠝࠠࠪࡦ㘃㧘᦭ᯏൻቇ‛⾰ߦ㑐ߔࠆ⎇ⓥ
⺞

ᩏ

⎇

ⓥ

ฬ

㘩ຠਛߩ࠳ࠗࠝࠠࠪࡦ㘃෸߮ߘߩ㑐ㅪൻว‛ߦ㑐ߔࠆ⺞ᩏ⎇ⓥ⎇ⓥ⠪ฬ㧔ᚲዻ㧕̪ ٤ශ㧦⎇ⓥઍ⴫⠪٤㘵↰㓉㓶㧔଻ஜ⑼ቇㇱ㐳㧕㧘ၳ ዞ⧷㧘⧃Ⴆ↱♿㧘ਛᎹ␞ሶ㧘ᐔᎹඳ઄㧔↢ᵴൻቇ⺖㧕㧘㘧⍹๺ᄢ㧔⸘᷹ᛛⴚ⺖㧕㧘૒‫ᧁޘ‬ਭ⟤ሶ㧔࿖┙ක⮎ຠ㘩ຠⴡ↢⎇ⓥᚲ㧕ᧄᐡ㑐ଥㇱ࡮⺖଻ஜ⑔␩ㇱડ↹⺖㧘↢ᵴⴡ↢⺖⺞ ᩏ ⎇ ⓥ ᦼ 㑆ᐔᚑ㧝㧠ᐕᐲ⺞ ᩏ ⎇ ⓥ ⒳ ⋡㧝㧚‫ⴕغ‬᡽⎇ⓥ‫⺖غ‬㗴⎇ⓥ‫౒ع‬ห⎇ⓥ㧔౒หᯏ㑐ฬ㧦‫غ‬ฃ⸤⎇ⓥ㧔ᆔ⸤ᯏ㑐ฬ㧦㧞㧚‫غ‬ၮ␆⎇ⓥ‫ع‬ᔕ↪⎇ⓥ㧟㧚‫غ‬㊀ὐ⎇ⓥ‫غ‬ផᅑ⎇ⓥ

㧙

㧝㧢ᐕᐲ

㧔㧟ᐕ㑆㧕
㧕
㧕
‫غ‬㐿⊒⎇ⓥ‫غ‬㧵㧿㧻ផㅴ⎇ⓥ߰ߊ߅߆ᣂ਎♿⸘↹╙㧞ᰴታᣉ⸘↹ᄢ㗄⋡㧦቟ోߢ቟ᔃߒߡ᥵ࠄߖࠆ↢ᵴߩ⏕଻ਛ㗄⋡㧦ᐔⓏߢ቟ోߥ⋵᳃↢ᵴߩ⏕଻ዊ㗄⋡㧦⼾߆ߢ቟ᔃߢ߈ࠆᶖ⾌↢ᵴߩ⏕଻⑔ጟ⋵ⅣႺ✚วၮᧄ⸘↹㧔㧼㧕̪ⅣႺ㑐ଥߩߺ

ᩇ 㧦
࠹࡯ࡑ㧦

ࠠ

Ԙ㘩ຠ

࡯

ࡢ

࡯

࠼

ԙ࠳ࠗࠝࠠࠪࡦ㘃⎇ⓥ

Ԛᄢ㊂⹜ᢱᵈ౉ᴺߩ᭎ԛ㜞ㅦṁᇦ᛽಴ᴺ

ⷐ㧝㧕⺞ᩏ⎇ⓥߩ⋡⊛෸߮ᔅⷐᕈ
㘩ຠਛߩ࠳ࠗࠝࠠࠪࡦ㘃ಽᨆߢⷐ᳞ߐࠇࠆᬌ಴ਅ㒢୯ߪ 0.01 㨪 1pg/g ߢ޽ࠆ㧚ߎࠇࠄߩᬌ಴ਅ㒢ࠍ㆐ᚑߔࠆߚ߼ߦ
ߪ㧘ᓥ೨ߩ᷹ቯᯏེߩᗵᐲ߆ࠄൊ᩺ߔࠆߣ㧘৻࿁ߩ᛽಴ߦ 100g એ਄ߩ⹜ᢱ߇ᔅⷐߢ޽ࠆ㧚ߎࠇߪ㘩ຠߩ࠳ࠗࠝࠠࠪ
ࡦಽᨆ߇ᄙߊߩഭജߣᤨ㑆ࠍᔅⷐߣߔࠆᚲએߢ޽ࠅ㧘ߔߥࠊߜ᷹ቯᯏེߩ㜞ᗵᐲൻߪಽᨆᎿ⒟ߩㄦㅦ࡮ല₸ൻߦᦨ߽ലᨐ⊛ߥᚻᲑߣ޿߃ࠆ㧚߹ߚ㧘㘩ຠਛߦ⷗޿ߛߐࠇࠆ࠳ࠗࠝࠠࠪࡦ㘃ߪኾࠄⅣႺᇦ૕ࠃࠅૐỚᐲߢ޽ࠅ㧘߶ߣࠎߤߩ
⇣ᕈ૕߇ᬌ಴㒢⇇એਅ㧘ታ᷹ߐࠇߡ߽ᬌ಴㒢⇇ઃㄭߢ޽ࠆߎߣ߇ߒ߫ߒ߫ߢ޽ࠆ㧚ᬌ಴㒢⇇ߦㄭߠߊ߶ߤቯ㊂♖ᐲߪᖡൻߔࠆߚ߼㧘ᬌ಴ᗵᐲߩᡷༀࠍ࿑ࠅ᷹ቯ୯ߩ♖ኒᕈ෸߮ା㗬ᕈࠍะ਄ߔࠆߚ߼ߩᬌ⸛߇ᔅⷐߢ޽ࠆ㧚㧞㧕⺞ᩏ⎇ⓥߩ᭎ⷐ㜞ಽ⸃⢻ GC/MS ߦ߅ߌࠆ࠳ࠗࠝࠠࠪࡦ㘃᷹ቯߩ㜞ᗵᐲൻࠍ⋡⊛ߣߒߡ 㧘‫ޟ‬ṁᇦ㒰෰࡮ᄢ㊂⹜ᢱᵈ౉ᴺ ‫ޠ‬㧔એਅ
SCLV㧕ߩㆡ↪ᕈߣᦨㆡ᧦ઙࠍᬌ⸛ߔࠆ㧚ߐࠄߦ㧘᛽಴ᠲ૞ߩ⥄േ㧔ᯏ᪾㧕ൻ࡮ㄦㅦൻࠍ⋡⊛ߣߒ 㧘‫ޟ‬㜞ㅦṁᇦ᛽಴
ᴺ‫ޠ‬㧔એਅ ASE㧕ߩㆡ↪ᕈࠍᬌ⸛ߔࠆ㧚ߎࠇࠄࠍ⚵ߺวࠊߖ㧘㘩ຠਛ࠳ࠗࠝࠠࠪࡦ㘃ߩ╙ੑ਎ઍߩ㜞ᗵᐲㄦㅦᓸ㊂ಽ
ᨆᣇᴺߣߒߡ⏕┙ߔࠆ㧚⏕┙ߒߚಽᨆᣇᴺࠍ↪޿ߡ㧘㘩ຠਛߩ࠳ࠗࠝࠠࠪࡦ㘃ᳪᨴᐲ⺞ᩏࠍታᣉߔࠆ㧚㧟㧕⺞ᩏ⎇ⓥߩ㆐ᚑᐲ෸߮ᓧࠄࠇߚᚑᨐ㧔ߢ߈ࠆߛߌᢙ୯ൻߒߡߊߛߐ޿㧕Ԙ㘩ຠਛ࠳ࠗࠝࠠࠪࡦ㘃ߩ㜞ᗵᐲㄦㅦಽᨆᣇᴺߩ㐿⊒SCLV ߣ㐳ߐ 40m ߩ㜞ᭂᕈ Rtx-2330 ࠞ࡜ࡓࠍ⚵ߺวࠊߖߡ㧘ᦨㆡߥ⸘᷹᧦ઙࠍᬌ⸛ߒߚߣߎࠈ㧘ᓥ᧪ߩᣇᴺࠃࠅఝࠇߚࡇ࡯ࠢಽ㔌ߣᗵᐲ߇ᓧࠄࠇ㧘ᬌ಴ᗵᐲߪో⇣ᕈ૕ߦਗ਼ࠅ 5 㨪 10 ୚ะ਄ߒߚ㧚ߘߎߢ ASE ߩᬌ⸛ߢߪ⹜ᢱណข㊂ࠍ
20g㧔ᓥ᧪Ყ 5 ಽߩ 1㧕ߦ⸳ቯߒ㧘SCLV ߣ ASE ߦࠃࠆ‫ޟ‬ᡷ⦟ᴺ‫ޟߣޠ‬ᓥ᧪ᴺ‫ߢߣޠ‬ታ᷹୯ࠍᲧセߔࠆห╬ᕈ⹜㛎㧔ࡃ࡝࠺࡯࡚ࠪࡦ㧕ࠍ㧘ᬀ‛ᕈ㧔ੇᶏ⧡㧕෸߮േ‛ᕈ㘩ຠ㧔㝼น㘩ㇱ㧕ࠍ↪޿ߡታᣉߒߚ㧚ߘߩ⚿ᨐ㧘ᡷ⦟ᴺߢᓧࠄ
ࠇࠆታ᷹୯ߪᓥ᧪ᴺࠃࠅ߽ో⥸ߦ㜞޿௑ะࠍ␜ߒߚ㧚ߎߩߎߣߪ ASE ߦ߅ߌࠆ᛽಴ല₸ߩ㜞ߐࠍ෻ᤋߒߚ߽ߩߣ⠨߃ࠄࠇߚ㧚ᧄᴺߦ߅ߌࠆ࠳ࠗࠝࠠࠪࡦ㘃ߩᷝട࿁෼₸ࠍ⒳‫ߩޘ‬㘩ຠ⹜ᢱߢ⺞ߴߚߣߎࠈ㧘ᓥ᧪ߩṁᇦᝄߣ߁᛽಴ᴺߣ
ห╬એ਄ߢ޽ࠅ㧘‫ޟ‬㘩ຠਛߩ࠳ࠗࠝࠠࠪࡦ㘃ಽᨆᣇᴺࠟࠗ࠼࡜ࠗࡦ‫⸥ߦޠ‬タߐࠇߡ޿ࠆା㗬ᕈ଻⸽୯㧔40 㨪 120 㧑㧕ߩ▸࿐ౝߢ޽ߞߚ㧚એ਄ߩ⚿ᨐ߆ࠄᧄᣇᴺߪ㘩ຠਛ࠳ࠗࠝࠠࠪࡦ㘃ߩಽᨆᴺߣߒߡㆡ↪น⢻ߢ޽ࠆߎߣ߇ታ⸽ߐࠇ
ߚ㧚ԙ࿖ౝᵹㅢ㘩ຠߩ࠳ࠗࠝࠠࠪࡦ㘃ᳪᨴᐲ⺞ᩏ࿖┙ක⮎ຠ㘩ຠⴡ↢⎇ⓥᚲߣ౒หߢ࿖ౝᵹㅢ㘩ຠߩࠨࡦࡊ࡝ࡦࠣ⺞ᩏࠍታᣉߒߚ㧚⺞ᩏ⹜ᢱᢙߪ㧘ᐔᚑ 14 ᐕᐲ 209㧔ᒰ⎇ⓥᚲಽᨆᢙ 77㧕㧘ᐔᚑ 15 ᐕᐲ 170㧔52㧕ߢ޽ࠆ㧚㧠㧕⋵᳃ߩஜᐽߩ଻ᜬ෶ߪⅣႺߩ଻ో߳ߩ⽸₂㘩ຠਛߩ᦭ኂ‛⾰ࠍ♖ኒߦ⸘᷹ߒߡ࠺࡯࠲ࠍ⫾Ⓧߔࠆߎߣ㧘ߐࠄߦㄦㅦߦಽᨆߔࠆߚ߼ߩᣇᴺࠍ㐿⊒ߔࠆߎߣߪ㧘⋵᳃ߩஜᐽⵍኂࠍᧂὼߦ㒐ߋߎߣߦ❬߇ࠅ㧘㘩ຠߩᳪᨴ໧㗴߇⊒↢ߒߚ႐วߩ౞Ṗߢㅦ߿߆ߥⴕ᡽ኻᔕߦነਈߔࠆ㧚㧡㧕⺞ᩏ⎇ⓥ⚿ᨐߩ⁛ഃᕈ㧘ᣂⷙᕈ
௖߆ 20 ࠣ࡜ࡓߩ㘩ຠ߆ࠄ㜞ᗵᐲߦ㘩ຠਛ࠳ࠗࠝࠠࠪࡦ㘃ߩᬌ಴ࠍน⢻ߣߒߚὐߦᦨᄢߩᣂⷙᕈ߇޽ࠆ㧚ㄦㅦᕈ߇ะ਄ߒߚ߶߆㧘ⅣႺ㕙࡮ࠦࠬ࠻㕙ߢߪ᛽಴ṁᇦ㊂ߩ⚂ 60 㧑೥ᷫࠍ㆐ᚑߒߡ޿ࠆ㧚㧢㧕ᚑᨐߩᵴ↪⁁ᴫ㧔ᛛⴚ⒖ォ࡮ᵴ↪ߩน⢻ᕈ㧕㐿⊒ߐࠇߚᣇᴺߪᧄ⎇ⓥᚲߦ߅ߌࠆ㘩ຠਛߩ࠳ࠗࠝࠠࠪࡦ㘃ಽᨆᴺߣߒߡᣢߦㆇ↪ߐࠇߡ޿ࠆ㧚࿖ౝᄖߩಽᨆᯏ㑐╬߳ߩᛛⴚ⒖ォ߇ᦼᓙߐࠇࠆ㧚 

⚳ੌ㧟⺞ᩏ⎇ⓥ⚳ੌႎ๔ᦠ
⎇ⓥಽ㊁㧦࠳ࠗࠝࠠࠪࡦ㘃㧘᦭ᯏൻቇ‛⾰ߦ㑐ߔࠆ⎇ⓥ
⺞

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⎇

ⓥ

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㘩ຠਛ᦭ኂ⥇⚛ൻว‛ߩᳪᨴታᘒߩ⸃᣿ߦ㑐ߔࠆ⎇ⓥ⎇ⓥ⠪ฬ㧔ᚲዻ㧕̪ ٤ශ㧦⎇ⓥઍ⴫⠪٤ਛᎹ␞ሶ , ⧃Ⴆ↱♿ , ၳ ዞ⧷ ,㘵↰㓉㓶㧔଻ஜ⑼ቇㇱ㐳㧕ᧄᐡ㑐ଥㇱ࡮⺖଻ஜ⑔␩ㇱડ↹⺖,↢ᵴⴡ↢⺖⺞ ᩏ ⎇ ⓥ ᦼ 㑆ᐔᚑ㧝㧠ᐕᐲ㧝㧢ᐕᐲ⺞ ᩏ ⎇ ⓥ ⒳ ⋡㧝㧚‫ⴕغ‬᡽⎇ⓥ‫⺖غ‬㗴⎇ⓥ‫౒غ‬ห⎇ⓥ㧔౒หᯏ㑐ฬ㧦‫ع‬ฃ⸤⎇ⓥ㧔ᆔ⸤ᯏ㑐ฬ㧦㧞㧚‫ع‬ၮ␆⎇ⓥ‫غ‬ᔕ↪⎇ⓥ㧟㧚‫غ‬㊀ὐ⎇ⓥ‫غ‬ផᅑ⎇ⓥ

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㘧⍹๺ᄢ,

㧔㧟ᐕ㑆㧕
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‫غ‬㐿⊒⎇ⓥ‫غ‬㧵㧿㧻ផㅴ⎇ⓥ߰ߊ߅߆ᣂ਎♿⸘↹╙㧞ᰴታᣉ⸘↹ᄢ㗄⋡㧦቟ోߢ቟ᔃߒߡ᥵ࠄߖࠆ↢ᵴߩ⏕଻ਛ㗄⋡㧦ᐔⓏߢ቟ోߥ⋵᳃↢ᵴߩ⏕଻ዊ㗄⋡㧦⼾߆ߢ቟ᔃߢ߈ࠆᶖ⾌↢ᵴߩ⏕଻⑔ጟ⋵ⅣႺ✚วၮᧄ⸘↹㧔㧼㧕̪ⅣႺ㑐ଥߩߺ

ᩇ 㧦
࠹࡯ࡑ㧦

ࠠ

Ԙ⥇⚛ൻ࠳ࠗࠝࠠࠪࡦ

࡯

ࡢ

࡯

࠼

⎇

ᐔᎹඳ઄㧘┻ਛ㊀ᐘ㧔↢ᵴൻቇ⺖㧕,ԙ⥇⚛ൻࠫࡈࠚ࠾࡞ࠛ࡯࠹࡞
ⓥ

ߩ

᭎

Ԛ㘩ຠ

ԛ៨ข㊂

Ԝ⹏ଔ

ⷐ

㧝㧕⺞ᩏ⎇ⓥߩ⋡⊛෸߮ᔅⷐᕈ
ࡊ࡜ࠬ࠴࠶ࠢߩ㔍Ά೷ߣߒߡᷝടߐࠇࠆ⥇⚛♽ൻว‛ߦߟ޿ߡߪ㧘ߘߩ⵾ㅧㆊ⒟߿ߘࠇࠄࠍ฽߻ࡊ࡜ࠬ࠴࠶ࠢߩ὾
ළㆊ⒟ߦࠃࠅ⥇⚛ൻ࠳ࠗࠝࠠࠪࡦ߇↢ᚑߔࠆߎߣ᣿ࠄ߆ߦߐࠇߡ޿ࠆ߇㧘⥇⚛ൻ࠳ࠗࠝࠠࠪࡦ෸߮ߘߩ⥇⚛♽㔍Ά೷․ߦ⥇⚛ൻࠫࡈࠚ࠾࡞ࠛ࡯࠹࡞ߦࠃࠆ㘩ຠᳪᨴߦ㑐ߒߡ⃻࿷߹ߢߦᓧࠄࠇߡ޿ࠆ࠺࡯࠲ߪዋߥ޿㧚ߘߎߢ㧘ߎࠇࠄߩൻቇ‛⾰ߩ㜞ᗵᐲߢ㜞♖ᐲߩᓸ㊂ಽᨆᴺࠍ⏕┙ߒ㧘ᳪᨴߩታᘒࠍ᣿ࠄ߆ߦߒ㧘ࡅ࠻߳ߩᦑ㔺㊂ߦߟ޿ߡ⹏ଔߔࠆߎߣ
߇㘩ຠߩ቟ో⏕଻ᣉ╷ߩ਄ߢᕆോߣߥߞߡ޿ࠆ㧚㧞㧕⺞ᩏ⎇ⓥߩ᭎ⷐᧄ⎇ⓥߪԘᓸ㊂ಽᨆᴺߩ⏕┙㧘ԙᧄಽᨆᴺࠍ↪޿ߚታ㓙ߩ㘩ຠ⹜ᢱߦ߅ߌࠆ⥇⚛ൻ࠳ࠗࠝࠠࠪࡦ෸߮⥇⚛ൻࠫࡈࠚ࠾࡞ࠛ࡯࠹࡞ߩᳪᨴታᘒ⺞ᩏ߆ࠄߥࠆ㧚⺞ᩏኻ⽎ߩ㘩ຠ⹜ᢱߪ୘೎࡮ᮨᡆ㘩੐⹜ᢱ࡮㒶⤝㘩੐⹜ᢱߥߤߢ޽ࠆ㧚ߎࠇࠄߩ࠺࡯࠲ࠍၮߦࡅ࠻߳ߩᦑ㔺㊂ࠍផቯߒ࡝ࠬࠢ⹏ଔࠍⴕ޿㧘㘩ຠߩ቟ో⏕଻ᣉ╷ߦ⾗ߔࠆ㧚㧟㧕⺞ᩏ⎇ⓥߩ㆐ᚑᐲ෸߮ᓧࠄࠇߚᚑᨐ㧔ߢ߈ࠆߛߌᢙ୯ൻߒߡߊߛߐ޿㧕Ԙ㘩ຠߦ߅ߌࠆ⥇⚛ൻ࠳ࠗࠝࠠࠪࡦ 2$&&(U ෸߮ߘߩ㑐ㅪൻว‛⾰ߢ޽ࠆ⥇⚛ൻࠫࡈࠚ࠾࡞ࠛ࡯࠹࡞(PBDEs)ߩห
ᤨ೨ಣℂߦࠃࠆㄦㅦ߆ߟᓸ㊂ಽᨆࠍ⏕┙ߒ㧘ቇⴚ⹹ߦᛩⓂߒߚ㧚ԙ⏕┙ߒߚᣇᴺߦࠃࠅ㧘↢㞲㝼੺㘃෸߮ߘߩടᎿຠࠍਥߣߒߚ୘೎㘩ຠ⹜ᢱ㧘෸߮ࡑ࡯ࠤ࠶࠻ࡃࠬࠤ࠶࠻㧔/$㧕㘩ຠ⟲⹜ᢱ㨪⟲⸘⹜ᢱ㧘߹ߚ㧘ฬߩࡏ࡜ࡦ࠹ࠖࠕ߆ࠄ㓸߼ߚ㨪ᣣಽߩ㘩੐⹜ᢱࠍಽᨆߒߚ㧚೨⠪ߩ୘೎㘩ຠߩಽᨆ⚿ᨐ߆ࠄߪ㧘2$&&(U෸߮ PBDEs ߦࠃࠆⅣႺᳪᨴᐲ߇㧘ᓟ⠪ߩ/$⹜ᢱ߿㘩੐⹜ᢱߩಽᨆ⚿ᨐ߆ࠄߪ㧘ࡅ࠻ߩᣣ
៨ข㊂㧔ᦑ㔺㊂㧕ࠍផቯߔࠆߎߣ߇ߢ߈ߚ㧚Ԛᧄ⎇ⓥߩ⚿ᨐ㧘୘೎㘩ຠߩᢙ଀߆ࠄߩߺᬌ಴ߐࠇߚ⥇⚛♽࠳ࠗࠝࠠࠪࡦߩỚᐲߪᐔᚑᐕᐲෘ↢ഭ௛⑼ቇ⎇ⓥߢႎ
๔ߐࠇߚ↢㞲㝼੺ਛႮ⚛♽࠳ࠗࠝࠠࠪࡦߩᐔဋỚᐲRI6'3IߦᲧߴએਅߣૐ߆ߞߚ㧚ԛ⥇⚛ൻࠫࡈࠚ࠾࡞ࠛ࡯࠹࡞ߪ㧘ࠞࡢࡂࠡએᄖߩ↢㞲㝼੺ߦᦨ㜞ߢRII㧘ᶏ⨲෸߮㝼੺ടᎿ㘩ຠߪ㨪 RI
Iᬌ಴ߐࠇߚ㧚/$㘩ຠ⟲⹜ᢱߪ╙㧢⟲㧔ᨐታ㧕એᄖߩߔߴߡߩ⹜ᢱߦᬌ಴ߐࠇ㧘៨ข㊂ߪPIᣣߣផቯߐࠇߚ㧚৻ᣇ㧘ࡏ࡜ࡦ࠹ࠖࠕߩ㘩੐⹜ᢱߦߟ޿ߡ߽ߔߴߡ߆ࠄᬌ಴ߐࠇߚ߇㧘ᦨዊᲥᕈ㊂.1#'. OIMI૕㊀ᣣ ࠍ⿥߃ߥ߆ߞ
ߚ㧚㧠㧕⋵᳃ߩஜᐽߩ଻ᜬ෶ߪⅣႺߩ଻ో߳ߩ⽸₂
ᧄ⎇ⓥߩኻ⽎ߢ޽ࠆ⥇⚛ൻ‛ߪ㧘ࡊ࡜ࠬ࠴࠶ࠢ⵾ຠߩ㔍Άᕈࠍ㜞߼ࠆߚ߼ߦᷝടߐࠇࠆ߽ߩߢ㧘ᱷ⇐ᕈ࡮⫾Ⓧᕈ߇
㜞޿߽ߩ߇޽ࠆ㧚ߎࠇࠄߦࠃࠆ㘩ຠ߳ߩᳪᨴࠍ㒐ᱛߔࠆߚ߼ߩ㧘ᣧ޿Ბ㓏߆ࠄߩ⋙ⷞߪ㧘ᧄ‛⾰ߦࠃࠆ᭽‫ߥޘ‬ⅣႺᳪ
ᨴߥߤߩ␠ળ໧㗴ߦහᔕߔࠆߎߣ߇ߢ߈㧘ⴕ᡽਄᦭⋉ߣߥࠆ㧚㧡㧕⺞ᩏ⎇ⓥ⚿ᨐߩ⁛ഃᕈ㧘ᣂⷙᕈᧄ⎇ⓥߪ㜞ಽ⸃⢻⾰㊂ಽᨆ⸘ࠍ᦭ߒ㧘࠳ࠗࠝࠠࠪࡦߢ㜞޿ᛛⴚ߇଻⸽ߐࠇߚᒰ⎇ⓥᚲߢೋ߼ߡน⢻ߣߥࠆ࠹࡯ࡑߢ
޽ࠅ㧘⥇⚛ൻ‛ࠍಽᨆኻ⽎ߦߒߡ޿ࠆᯏ㑐ߪ࿾ⴡ⎇㧘౏ኂ⎇㧘ᄢቇߩਛߢ߽ዋߥߊ㧘ኾ㐷ᕈߩὐߢ㧘ో࿖࡟ࡌ࡞ߢఝ
૏ߦ૏⟎ߒߡ޿ࠆ㧚ߎߩߎߣ߆ࠄ߽ౝኈ⊛ߦ⁛ഃᕈ࡮ᣂᄸᕈ߇㜞޿㧚㧢㧕ᚑᨐߩᵴ↪⁁ᴫ㧔ᛛⴚ⒖ォ࡮ᵴ↪ߩน⢻ᕈ㧕⃻Ბ㓏ߢߪࡕ࠾࠲࡝ࡦࠣ߇ਥߢ޽ࠆߚ߼㧘ಽᨆᛛⴚ߇ᛛⴚ⒖ォߥߤߩኻ⽎ߣߥࠆ㧚 

⺞ᩏ⎇ⓥ⚳ੌႎ๔ᦠ

⎇ⓥಽ㊁㧦
⺞

ᩏ

⎇

ⓥ

ฬ

⑔ጟ⋵ߦ߅ߌࠆਥⷐᱫ࿃ಽᨆ㧔1973 ᐕ߆ࠄ 2002 ᐕߩ 30 ᐕ㑆ߩ߹ߣ߼㧕⎇ⓥ⠪ฬ㧔ᚲዻ㧕̪ ٤ශ㧦⎇ⓥઍ⴫⠪٤ᧄᐡ㑐ଥㇱ࡮⺖଻ஜ⑔␩ㇱડ↹⺖⺞ ᩏ ⎇ ⓥ ᦼ 㑆ᐔᚑ㧝㧡ᐕᐲ⺞ ᩏ ⎇ ⓥ ⒳ ⋡㧝㧚‫ⴕع‬᡽⎇ⓥ‫⺖غ‬㗴⎇ⓥ‫౒غ‬ห⎇ⓥ㧔౒หᯏ㑐ฬ㧦‫غ‬ฃ⸤⎇ⓥ㧔ᆔ⸤ᯏ㑐ฬ㧦㧞㧚‫غ‬ၮ␆⎇ⓥ‫ع‬ᔕ↪⎇ⓥ㧟㧚‫غ‬㊀ὐ⎇ⓥ‫ع‬ផᅑ⎇ⓥ

ጟᕶ৻㇢㧘ዊ㊁Ⴆᄢ੺㧘㜟የ૫ሶ㧘㜞ጊᶈ᮸㧔ᖱႎ▤ℂ⺖㧕㧘଻ஜ⑔␩ㇱડ↹⺖ᖱႎ⛔⸘ଥ

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㧝㧢ᐕᐲ

㧔㧞ᐕ㑆㧕
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‫غ‬㐿⊒⎇ⓥ‫غ‬㧵㧿㧻ផㅴ⎇ⓥ߰ߊ߅߆ᣂ਎♿⸘↹╙㧞ᰴታᣉ⸘↹ᄢ㗄⋡㧦ሶߤ߽߿㜞㦂⠪ߣ↢߈↢߈᥵ࠄߔஜᐽ␠ળߠߊࠅ
ਛ㗄⋡㧦⋵᳃ߩஜᐽ⊛ߥ᥵ࠄߒߠߊࠅߩផㅴ
ዊ㗄⋡㧦⥄ਥ⊛ஜᐽߠߊࠅߩផㅴ

⑔ጟ⋵ⅣႺ✚วၮᧄ⸘↹㧔㧼㧕̪ⅣႺ㑐ଥߩߺ

ᩇ 㧦
࠹࡯ࡑ㧦

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Ԙ㩄㩧㩕㩩㨷㨺㩊㩆㩇㩍㩛

࡯

ࡢ

࡯

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⚳ੌ㧠

ԙ EBSMR Ԛᱫ࿃
⎇

ⓥ

ߩ

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ԛᐕ㦂⺞ᢛᱫ੢₸ⷐ㧝㧕⺞ᩏ⎇ⓥߩ⋡⊛෸߮ᔅⷐᕈ
ᤘ๺ᐕ㧔㧕߆ࠄᐔᚑᐕ㧔㧕߹ߢߩᐕ㑆ߦࠊߚࠆᧄ⋵ߩੱญേᘒ㧔ᱫ੢㧕⛔⸘߆ࠄᱫ੢₸ࠍ࿾ℂ⊛
㧔Ꮢ඙↸᧛೎㧕㧘ᤨ♽೉⊛ߦ᳞߼㧘࿾ၞᏅߣᐕᰴᄌൻࠍಽᨆߒ㧘ᧄ⋵ߩᱫ੢᭴ㅧࠍ᣿ࠄ߆ߦߔࠆ㧚ᧄ⋵ߦ߅޿ߡ߽਎♿ߪዋሶ㜞㦂ൻ߇ᕆㅦߦㅴ߻㧚ߘߩࠃ߁ߥ⁁ᴫߩਛߢ㧘੃ᐜఽ߿㕍ჽᐕᦼߩᱫ੢ࠍᛥ೙ߒ㧘߹ߚ㧘㜞㦂⠪߇޿߈޿߈ߣ⼾߆ߥ↢ᵴࠍㅍࠇࠆ␠ળⅣႺߩ᭴▽ߪߎࠇ߆ࠄߩ଻ஜ⑔␩ⴕ᡽ߩᩮᐙߢ޽ࠆ㧚ߎߩᱫ࿃ಽᨆ
⎇ⓥߪ⋵ౝᏒ඙↸᧛ߩ੃ᐜఽ߆ࠄ㜞㦂⠪߹ߢߩᱫ੢⁁ᴫࠍ⹏ଔߔࠆ߽ߩߢ޽ࠅ㧘↢ᵴ⠌ᘠ∛╬ߩஜᐽኻ╷ࠍផㅴߔࠆ
਄ߢၮ␆⊛ߥ⾗ᢱࠍᓧࠆߚ߼ߦߪᔅⷐਇนᰳߥ⎇ⓥߢ޽ࠆ㧚㧞㧕⺞ᩏ⎇ⓥߩ᭎ⷐ‫଻ޣ‬ஜ⛔⸘ᐕႎ㩆㩇㩍㩛ߩౣ᭴▽‫⎇ߩߎ ޤ‬ⓥߩᱫ੢㩍㩨㨺㩊ߪᧄ⋵ߩ଻ஜ⛔⸘ᐕႎ㧔ੱญേᘒ⛔⸘㧕ࠍ૞ᚑߔࠆㆊ⒟ߢᓧࠄ
ࠇࠆ⾗ᢱࠍၮߦᛠីߒߡ߈ߚ㧚⃻࿷ߘߩ଻ஜ⛔⸘ᐕႎ૞ᚑಣℂߪ᳢↪㩄㩧㩕㩩㨷㨺㩊㩆㩇㩍㩛ࠍ↪޿ߡⴕࠊࠇߡ߅ࠅ㧘ߘߩ಴ജᒻᘒߪኾ↪᭽ᑼ߳ߩශ೚‛ߣߒߡߩឭଏߩߺߢ޽ࠆ㧚㩖㨱㨼㩣಴ജ╬ߩᦨㄭߩⴕ᡽ⷐᦸߦኻಣߔࠆߚ߼ߦ㧘ᓥ᧪ߩಣℂ㩆㩇㩍㩛߆ࠄᣂߚߥ⸒⺆ߢߩಣℂ㩆㩇㩍㩛ࠍ᭴▽ߔࠆ㧚‫ޣ‬ᐕ㑆ߦ߅ߌࠆ⑔ጟ⋵ߩਥⷐᱫ࿃ಽᨆ‫ ޤ‬ᣂ㩆㩇㩍㩛ߦᐕ㑆ߩᱫ੢㩍㩨㨺㩊㩗㩨㨺㩇ࠍ᭴▽ߒ㧘ᐕ㑆ࠍ-ᐕ㧘㧙ᐕ㧘㧙ᐕ㧘㧙㧘㧙ᐕ෸߮㧙ᐕߩᦼߦಽ㘃ߒ㧘ᐕ㦂⺞ᢛߣ࿾ၞߩੱญⷙᮨ
ࠍ⠨ᘦߒߚࡌࠗ࠭ផቯߦࠃࠆ SMR ᜰᮡࠍ↪޿ߡᱫ࿃ಽᨆࠍⴕ޿㧘ᧄ⋵ߩᱫ࿃᭴ㅧߩᄌൻߣో૕௝ࠍ⠨ኤߔࠆ㧚㧟㧕⺞ᩏ⎇ⓥߩ㆐ᚑᐲ෸߮ᓧࠄࠇߚᚑᨐ㧔ߢ߈ࠆߛߌᢙ୯ൻߒߡߊߛߐ޿㧕‫଻ޣ‬ஜ⛔⸘ᐕႎ㩆㩇㩍㩛ߩౣ᭴▽‫ ޤ‬Windows ߢࠪࠬ࠹ࡓࠍ᭴▽ߒߚ㧚࠺࡯࠲ࡌ࡯ࠬߪ㧘ⶄᢙߩ⠪߇หᤨߦ࠺࡯࠲ߩෳ
ᾖࠍⴕ߃ࠆࠃ߁ߦ SQLServer ߢ᭴▽ߒߚ㧚࠺࡯࠲ߩෳᾖ߅ࠃ߮ශ೚ߦߪ㧘߶ߣࠎߤߩࠦࡦࡇࡘ࡯࠲ߦࠗࡦࠬ࠻࡯࡞ߐ
ࠇߡ޿ࠆࠛࠢ࠮࡞ࠍ೑↪ߒߚ㧚૞ᚑน⢻ߣߥߞߚੱญേᘒ⛔⸘⴫ߪ✚ⷩ⒳㘃㧘಴↢߇⒳㘃㧘ᱫ੢߇⒳㘃㧘ᱫ↥࡮๟↥ᦼ߇⒳㘃㧘ᇕᆪ࡮㔌ᇕ߇⒳㘃ߢ޽ࠆ㧚‫ޣ‬ᐕ㑆ߦ߅ߌࠆ⑔ጟ⋵ߩਥⷐᱫ࿃ಽᨆ‫⑔ ޤ‬ጟ⋵ߦ߅ߌࠆᐕ߆ࠄᐕ߹ߢߩᐕ㑆ߩᱫ੢ᢙߪੱߛ
ߞߚ㧔↵ੱ‫ޔ‬ᅚੱ㧕㧚ᐕᐔဋੱ㧔↵ੱ‫ޔ‬ᅚੱ㧕ߦߥࠆ㧚ᐕ㦂⺞ᢛᱫ੢₸㧔㑆ធᴺ㧕ߩ⚿ᨐ㧘ᱫ੢✚ᢙ㧘ᖡᕈᣂ↢‛߿⢖Ἳ෸߮᳇▤ᡰἻߪẋჇ௑ะࠍ␜ߒߚ㧘⣖ⴊ▤∔ᖚ㧘㜞ⴊ࿶ᕈ∔ᖚ㧘ో⚿ᩭߪᷫዋ௑ะࠍ␜ߒߚ㧚EBSMR ߦࠃࠆ࿾ၞ⹏ଔߩ⚿ᨐ㧘ᖡᕈᣂ↢‛✚ᢙ߇એ਄ߩᏒ↸᧛߇㆙
⾐Ꮉᵹၞߣ╳ᓟᎹ࡮᦭᣿ᴪጯ࿾ၞߦ㓸Ⓧ௑ะ߇޽ߞߚ㧚㧠㧕⋵᳃ߩஜᐽߩ଻ᜬ෶ߪⅣႺߩ଻ో߳ߩ⽸₂଻ஜ⛔⸘ᐕႎߪ଻ஜⴕ᡽ࠍផㅴߒߡ޿ߊߚ߼ߩၮ␆⾗ᢱߣߒߡਇนᰳߥ߽ߩߢ޽ࠆ㧚ߎߩ࠺࡯࠲ࡌ࡯ࠬࠍ᭴▽ߔࠆߎߣߦࠃࠅ㧘ᄙⷺ⊛ߦ㧘ᱜ⏕ߦ㧘ㄦㅦߦ⑔ጟ⋵ߩᏒ↸᧛೎ߦ⹦⚦ߥ⛔⸘⴫߇૞ᚑน⢻ߦߥࠅ㧘ߘߩ࠺࡯࠲ࠍಽᨆߔࠆ
ߎߣߦࠃࠅஜᐽᣉ╷߳ߩឭ⸒ߦߟߥ߇ࠅ㧘᡽╷ߦ↢߆ߔߎߣߦࠃࠅ⋵᳃ߩஜᐽߩ଻ᜬߦ⽸₂ߢ߈ࠆ㧚㧡㧕⺞ᩏ⎇ⓥ⚿ᨐߩ⁛ഃᕈ㧘ᣂⷙᕈ
SQL ࠨ࡯ࡃ࡯਄ߢߩ࠺࡯࠲ࡌ࡯ࠬߩ᭴▽ߪᐢߊⴕࠊࠇߡ޿ࠆ߇㧘ߘߩᔕ↪ߣߒߡᧄ⋵ߦ߅޿ߡೋ߼ߡ଻ஜ⛔⸘ᐕႎࠪࠬ࠹ࡓ߇᭴▽ߐࠇߚ㧚ߘߩ࠺࡯࠲ࠍ↪޿ߚᱫ੢₸ߦ㑐ߔࠆ࿾ၞ⹏ଔߦߟ޿ߡߪᓥ᧪ߩ SMR ᜰᮡߦᄌ߃ߡ㧘ᣂߚߦ EBSMR Empirical bayes SMR ࠍណ↪ߒߚ㧚ߎߩᜰᮡߪᐕ㗃߆ࠄਤᓟࠄࠍਛᔃߦ Bayes ⛔⸘ቇࠍᔕ↪ߒߚ SMR
ߩ⸘▚ᚻᴺߢ޽ࠆ㧚ᐕ㦂⺞ᢛߦട߃㧘ੱญⷙᮨࠍ⺞ᢛߢ߈ࠆߣߎࠈߦ․ᓽ߇޽ࠆ㧚㧢㧕ᚑᨐߩᵴ↪⁁ᴫ㧔ᛛⴚ⒖ォ࡮ᵴ↪ߩน⢻ᕈ㧕଻ஜ⛔⸘ᐕႎࠪࠬ࠹ࡓߪᧄ⋵ߩ଻ஜ⛔⸘ᐕႎ૞ᚑߦᵴ↪ߐࠇߚ㧚EBSMR ߪ଻ஜᚲ⡯ຬߩᖱႎಣℂߦᵴ↪ߢ߈ࠆ㧚 

⚳ੌ㧡⺞ᩏ⎇ⓥ⚳ੌႎ๔ᦠ
⎇ⓥಽ㊁㧦ᄢ᳇ⅣႺᳪᨴߣߘߩኻ╷ߦ㑐ߔࠆ⎇ⓥ
⺞

ᩏ

⎇

ⓥ

ฬ

ើ⊒ᕈ᦭ᯏൻว‛(VOC)ᳪᨴ⸃ᨆߦኻߔࠆࡄ࠶ࠪࡉࠨࡦࡊ࡝ࡦࠣᴺߩታ⸽ൻ⎇ⓥ⎇ⓥ⠪ฬ㧔ᚲዻ㧕̪ ٤ශ㧦⎇ⓥઍ⴫⠪٤ᩉᎹᱜ↵㧘ᄢ⍹⥝ᒄ㧘ജᧄᐡ㑐ଥㇱ࡮⺖ⅣႺ଻ో⺖⺞ ᩏ ⎇ ⓥ ᦼ 㑆ᐔᚑ㧝㧠ᐕᐲ⺞ ᩏ ⎇ ⓥ ⒳ ⋡㧝㧚‫ⴕع‬᡽⎇ⓥ‫⺖غ‬㗴⎇ⓥ‫౒غ‬ห⎇ⓥ㧔౒หᯏ㑐ฬ㧦‫غ‬ฃ⸤⎇ⓥ㧔ᆔ⸤ᯏ㑐ฬ㧦㧞㧚‫غ‬ၮ␆⎇ⓥ‫غ‬ᔕ↪⎇ⓥ㧟㧚‫غ‬㊀ὐ⎇ⓥ‫غ‬ផᅑ⎇ⓥ㧙ኼ㓶㧘ጤᧄ⌀ੑ㧔ᄢ᳇⺖㧕

㧝㧢ᐕᐲ

㧔㧟ᐕ㑆㧕
㧕
㧕
‫ع‬㐿⊒⎇ⓥ‫ع‬㧵㧿㧻ផㅴ⎇ⓥ߰ߊ߅߆ᣂ਎♿⸘↹╙㧞ᰴታᣉ⸘↹ᄢ㗄⋡㧦࿾⃿⊛ⷞ㊁ߦ┙ߞߚᔟㆡⅣႺߩ଻ోߣഃㅧ
ਛ㗄⋡㧦ᔟㆡߥ↢ᵴⅣႺߩ଻ోዊ㗄⋡㧦߈ࠇ޿ߥⓨ᳇ߣ㕒߆ߥⅣႺߩ଻ో⑔ጟ⋵ⅣႺ✚วၮᧄ⸘↹㧔㧼㧕̪ⅣႺ㑐ଥߩߺᩇ 㧦↢ᵴⅣႺߩ଻ో࠹࡯ࡑ㧦ൻቇ‛⾰ߩㆡᱜߥ▤ℂࠠԘើ⊒ᕈ᦭ᯏൻว‛

࡯

ࡢ

࡯

࠼

ԙࡄ࠶ࠪࡉࠨࡦࡊ࡝ࡦࠣᴺ
⎇

ⓥ

ߩ

᭎

Ԛ᦭ኂൻቇ‛⾰ԛቶౝⓨ᳇ᳪᨴ

ⷐ㧝㧕⺞ᩏ⎇ⓥߩ⋡⊛෸߮ᔅⷐᕈ
ᦨㄭ㧘 PRTR ߩᴺ೙ൻ╬㧘ൻቇ‛⾰ߩㆡᱜ▤ℂߦะߌߚขࠅ⚵ߺ߇ㅴ߼ࠄࠇߡ޿ࠆ㧚߹ߚ㧘ើ⊒ᕈ᦭ᯏൻว‛(VOC)╬ߩ᦭ኂᄢ᳇ᳪᨴ‛⾰(HAPs)ߦࠃࠆ⊒߇ࠎᕈ╬ߩૐỚᐲ㐳ᦼ㑆᥸㔺ߩੱ૕ᓇ㗀߇ ᔨߐࠇ㧘ో࿖⊛ߥࡕ࠾࠲࡝
ࡦࠣ⺞ᩏ߇ⴕࠊࠇߡ޿ࠆ㧚HAPs ߩ㊀ⷐᚑಽߢ޽ࠆ VOC ߦߟ޿ߡߪ㧘⋵ౝߦ㧘ࡌࡦ࠯ࡦ㧘࠻࡝ࠢࡠࡠࠛ࠴࡟ࡦ㧘ࠫࠢࡠࡠࡔ࠲ࡦ╬ߩ㜞Ớᐲ࿾ၞ߇޽ࠅ㧘ߘࠇࠄߩ⊒↢Ḯ๟ㄝⅣႺ╬ߦ߅ߌࠆ㧘ߢ߈ࠆߛߌ㐳ᦼ㑆ߩ⹦⚦ߥᳪᨴ⁁ᴫߩᛠី߇᳞߼ࠄࠇߡ޿ࠆ㧚ᦝߦ㧘VOC ߦߟ޿ߡߪቶౝᳪᨴ╬ߩ໧㗴߽ᵈ⋡ߐࠇߡ޿ࠆ㧚ߘߩߚ߼㧘ᧄ⎇ⓥߢߪ㧘VOC ߦ㑐ߒߡ㧘৻⥸↢ᵴⅣႺ㧘⊒↢Ḯ๟ㄝ߆ࠄቶౝᳪᨴߩ໧㗴߹ߢ㧘⋵ౝߦ߅ߌࠆᳪᨴታᘒࠍ✚ว⊛ߦ᣿ࠄ߆ߦߒ㧘หᤨߦ◲ᤃߥ᷹ቯᣇᴺߣߒߡ㧘1 ߆᦬㑆⒟ᐲߩ㐳ᦼ㑆ߩ᝝㓸߇น⢻ߥࡄ࠶ࠪࡉࠨࡦࡊ࡝ࡦࠣᴺ㧔PS ᴺ㧕ߩታ⸽ൻࠍផㅴߔࠆߎ
ߣࠍ⋡⊛ߣߔࠆ㧚㧞㧕⺞ᩏ⎇ⓥߩ᭎ⷐ⋵ౝߩ VOC ᳪᨴ໧㗴ߦߟ޿ߡ㧘⊒↢Ḯ㧔Ꮏ႐㧕๟ㄝ߆ࠄ৻⥸ⅣႺ߅ࠃ߮ዬ૑ቶౝ߹ߢᐢ▸࿐ߩ⺞ᩏࠍⴕߞߚ㧚߹ߚ㧘ⅣႺᄢ᳇ਛߩ VOC ߦࠃࠆᳪᨴታᘒࠍᛠីߔࠆᚻᴺߣߒߡ㧘㐳ᦼ㑆᝝㓸↪ߩ PS ᴺ╬ߩታ↪ൻ㧘ታ⸽ൻߩߚ߼ߩ⎇ⓥࠍⴕߞߚ㧚ߐࠄߦ㧘PS ᴺࠍ↪޿ߡ⋵ౝฦ࿾ၞߩⅣႺᄢ᳇ਛߩ VOC ߦࠃࠆᳪᨴታᘒ⺞ᩏ╬ࠍⴕߞߚ㧚㧟㧕⺞ᩏ⎇ⓥߩ㆐ᚑᐲ෸߮ᓧࠄࠇߚᚑᨐ㧔ߢ߈ࠆߛߌᢙ୯ൻߒߡߊߛߐ޿㧕ᄢ—↰Ꮢౝ෸߮ਭ⇐☨Ꮢౝߩ⊒↢Ḯ๟ㄝⅣႺ㧘෸߮ᧄ⋵ౝߩ৻⥸↢ᵴⅣႺߦ߅޿ߡ㧘㐳ᦼ㑆㧔ㅳ㑆㨪߆᦬㑆㧕᝝㓸↪ߩ PS ᴺߩታ⸽⹜㛎෸߮㧘᜛ᢔࠪࡒࡘ࡟࡯࡚ࠪࡦ࠰ࡈ࠻ࠍ↪޿ߚ PS ᴺߣߩᲧセᬌ⸛╬ࠍⴕ޿㧘⦟ᅢߥ⚿ᨐ߇ᓧࠄࠇߚ㧚ߎߩ25ࠍ↪޿ߡ㧘ࡃ࠶ࠢࠣ࡜࠙ࡦ࠼࿾ၞࠍ฽߻⋵ౝ࿾ὐߦ߅޿ߡ㧘⚂߆᦬㑆ߩ㐳ᦼ㑆᝝㓸ࠍⴕ޿㧘⒳㘃
ߩ VOC ߩᳪᨴ⁁ᴫࠍ᣿ࠄ߆ߦߒߚ㧚߹ߚ㧘ቶౝⅣႺߦߟ޿ߡߪ㧘▽ᐕએౝߩᣂ▽૑ቛ㧔ోᚭ㧕ߦ߅ߌࠆቶౝᳪᨴ
⺞ᩏߩ⚿ᨐߩߣࠅ߹ߣ߼ࠍⴕ޿㧘ቶౝ VOC ߦࠃࠆᳪᨴߩታᘒ෸߮Ꮢ⽼ߩ PS ᴺߩㆡ↪น⢻▸࿐╬ࠍ᣿ࠄ߆ߦߒߚ㧚㧠㧕⋵᳃ߩஜᐽߩ଻ᜬ෶ߪⅣႺߩ଻ో߳ߩ⽸₂ૐỚᐲ㐳ᦼ㑆ߩ VOC ߦࠃࠆ⊒߇ࠎᕈ╬ߩ⋵᳃߳ߩஜᐽ࡝ࠬࠢࠍૐᷫߒ㧘ⅣႺ଻ోኻ╷ߩၮ␆⾗ᢱࠍ෼㓸ߔࠆߚ߼ߩ◲ଢߥ᷹ቯᚻᴺߣߒߡ PS ᴺࠍᵴ↪ߢ߈ࠆ㧚㧡㧕⺞ᩏ⎇ⓥ⚿ᨐߩ⁛ഃᕈ㧘ᣂⷙᕈߎߩ PS ᴺࠍ૶↪ߒߡ⋵ౝฦ࿾ၞߩ㧘৻⥸ⅣႺߦ߅ߌࠆᄙᢙ࿾ὐߢߩ㐳ᦼ㑆㧔⚂৻ࡩ᦬㑆㧕ߩ VOC ߩࠬࠢ࡝࡯࠾ࡦ
ࠣ⊛ߥ᷹ቯ⺞ᩏࠍ቟ଔߦታᣉߔࠆߎߣ߇น⢻ߢ޽ࠆ㧚߹ߚ㧘ᧄᴺߪ㧘VOC ⊒↢Ḯ๟ㄝⅣႺߦ߅޿ߡ߽㧘ᳪᨴߩ⒟ᐲ
ߦࠃࠆ߇㧘㨪ㅳ㑆⒟ᐲߩᐔဋ⊛ߥ VOC ߩᳪᨴ⁁ᴫࠍᛠីߔࠆߚ߼ߩ᷹ቯߦ߽૶↪น⢻ߣ⠨߃ࠄࠇࠆ㧚㧢㧕ᚑᨐߩᵴ↪⁁ᴫ㧔ᛛⴚ⒖ォ࡮ᵴ↪ߩน⢻ᕈ㧕੹࿁ᬌ⸛㧘㐿⊒ߒߚ PS ᴺߪ․⸵ߢߪߥ޿ߩߢ㧘౏ቯᴺߦᲧߴߡ቟ଔ㧔ࠨࡦࡊ࡜࡯୘⚂౞㧕ߦ⺕ߢ߽◲නߦᄙᢙࠍ㧘Ꮢ⽼ຠࠍ⚵ߺวࠊߖߡ⥄૞น⢻ߢ޽ࠆ㧚Ꮢ⽼ߩደᄖ↪ࠪࠚ࡞࠲࡯㧔୘౞㧕ࠍ૶↪ߒߡ㧘ደᄖߢߩ᳇᷷
߿Ḩᐲߩᄌൻ㧘㘑㔎╬ߩ᳇⽎᧦ઙߩ߽ߣߢ⚂߆᦬㑆ߩ㐳ᦼ㑆ߩ VOC ࠨࡦࡊ࡝ࡦࠣ߇น⢻ߢ޽ࠆ㧚߹ߚ㧘ߎߩࠨࡦࡊ
࡜࡯ߪ૶޿ᝥߡߢߪߥߊ㧘৻ㇱߩ᧚ᢱ㧔ࡈࠖ࡞࠲࡯ㇱಽ㧕ࠍ㒰߈ోߡౣ೑↪น⢻ߢ޽ࠅ㧘ⅣႺߦ߽ఝߒ޿․㐳߇޽ࠆ㧚੹ᓟ◲ଢߥ VOC ᝝㓸ᴺߣߒߡฦ⒳ VOC ߩࠬࠢ࡝࡯࠾ࡦࠣ⺞ᩏ߳ߩᵴ↪߇ᦼᓙߢ߈ࠆ㧚 

⚳ੌ㧢⺞ᩏ⎇ⓥ⚳ੌႎ๔ᦠ
⎇ⓥಽ㊁㧦ᄢ᳇ⅣႺᳪᨴߣߘߩኻ╷ߦ㑐ߔࠆ⎇ⓥ
⺞

ᩏ

⎇

ⓥ

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ᶋㆆ☸ሶ⁁‛⾰㧔SPM㧕ߦࠃࠆᄢ᳇ᳪᨴߦߟ޿ߡ
㧙⥄േゞឃ಴ࠟࠬߩᓇ㗀ࠍਛᔃߦ㧙⎇ⓥ⠪ฬ㧔ᚲዻ㧕̪ ٤ශ㧦⎇ⓥઍ⴫⠪٤᧼၂ᚑᵏ㧘ᄢ⍹⥝ᒄ㧘ጤᧄ⋿ੑ㧔ᄢ᳇⺖㧕ᧄᐡ㑐ଥㇱ࡮⺖ⅣႺ଻ో⺖⺞ ᩏ ⎇ ⓥ ᦼ 㑆ᐔᚑ㧝㧠ᐕᐲ⺞ ᩏ ⎇ ⓥ ⒳ ⋡㧝㧚‫ⴕع‬᡽⎇ⓥ‫⺖غ‬㗴⎇ⓥ‫౒غ‬ห⎇ⓥ㧔౒หᯏ㑐ฬ㧦‫غ‬ฃ⸤⎇ⓥ㧔ᆔ⸤ᯏ㑐ฬ㧦㧞㧚‫غ‬ၮ␆⎇ⓥ‫غ‬ᔕ↪⎇ⓥ㧟㧚‫غ‬㊀ὐ⎇ⓥ‫غ‬ផᅑ⎇ⓥ

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‫غ‬㐿⊒⎇ⓥ‫غ‬㧵㧿㧻ផㅴ⎇ⓥ߰ߊ߅߆ᣂ਎♿⸘↹╙㧞ᰴታᣉ⸘↹ᄢ㗄⋡㧦࿾⃿⊛ⷞ㊁ߦ┙ߞߚᔟㆡⅣႺߩ଻ోߣഃㅧ
ਛ㗄⋡㧦ᔟㆡߥ↢ᵴⅣႺߩ଻ోዊ㗄⋡㧦߈ࠇ޿ߥⓨ᳇ߣ㕒߆ߥⅣႺߩ଻ో⑔ጟ⋵ⅣႺ✚วၮᧄ⸘↹㧔㧼㧕̪ⅣႺ㑐ଥߩߺᩇ 㧦↢ᵴⅣႺߩ଻ో࠹࡯ࡑ㧦߈ࠇ޿ߥⓨ᳇ߩ⏕଻ࠠԘ⥄േゞឃ಴ࠟࠬ

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ԙᶋㆆ☸ሶ⁁‛⾰Ԛੑ㉄ൻ⓸⚛

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ԛ CalineԜ CMB㧝㧕⺞ᩏ⎇ⓥߩ⋡⊛෸߮ᔅⷐᕈ

ㄭᐕ㩍㩨㨲㨺㩈㩨㩣ゞឃ಴㩀㩨㩇ࠍේ࿃ߣߒߚ52/ߦࠃࠆᳪᨴ߇໧㗴ߣߥߞߡ޿ࠆ߇㧘⑔ጟ⋵ߦ߅޿ߡੑ㉄ൻ⓸⚛㧔01㧕෸߮
52/ߩⅣႺၮḰㆡว₸ߪߥ߆ߥ߆ᡷༀߐࠇߡ޿ߥ޿㧚ߎࠇ߹ߢ⥄േゞឃ಴ࠟࠬߦ㑐ߔࠆ⺞ᩏߪⴕࠊࠇߡ޿ࠆ߇㧘ᳪᨴ‛⾰Ớᐲߪ᳇⽎╬ߦᒝߊᓇ㗀ߐࠇࠆߚ߼㧘ᄢ᳇ᳪᨴ⁁ᴫߣ⥄േゞ⿛ⴕߩ㑐ଥߪචಽߦߪᛠីߐࠇߡ޿ߥ޿㧚․ߦ52/ߩ⊒↢Ḯߪ⥄േゞឃࠟࠬએᄖߦ࿯ფߩᏎ਄ߍ㧘ᶏႮ☸ሶߩ⒖ᵹ㧘෸߮ੑᰴ↢ᚑ☸ሶߥߤᄙ᭽ߢ޽ࠆߎߣ߆ࠄ⸃ᨆ߇࿎
㔍ߢ޽ߞߚ㧚ߘߎߢᧄ⎇ⓥߢߪ㧘⑔ጟ⋵ౝߢߩ⥄േゞ੤ㅢ㊂ߩಽᏓࠍᬌ⸛ߒߡߎߩਛ߆ࠄ⥄േゞឃ᳇ࠟࠬߩᓇ㗀ࠍฃߌ߿ߔ޿㆏〝ㄭற࿾ၞࠍ᛽಴ߒ㧘᳇⽎᧦ઙ߇ᄌൻߔࠆਛߢ⥄േゞឃ಴ࠟࠬߩᓇ㗀ࠍ᣿ࠄ߆ߦߔࠆߎߣࠍ⋡⊛ߣߒߚ㧚㧞㧕⺞ᩏ⎇ⓥߩ᭎ⷐ߹ߕ㧘⑔ጟ⋵ో૕ߩ੤ㅢ㊂ߩ⁁ᘒࠍᛠីߔࠆߚ߼㧘੤ㅢ㩈㩧㩅㩇╬ߩ㩍㩨㨺㩊ࠍᢛℂߒ㧘ᄙ‫⦟ޘ‬ᵺ᳓႐ߩઁᢙ࿾ὐࠍ᛽಴ߒ
ߚ㧚SPM ߦ㑐ߒߡ㧘㩔㨼㩘㩨㩢㨷㨺㩛㨾㨻㨺㩅㩧㩖㩩㩡㨺ࠍ↪޿ߡ㆏〝ㄭறߩ52/ࠍណขߒ㧘52/ਛߩ὇⚛ᚑಽ㧘㊄ዻᚑಽ㧘߅ࠃ߮᳓ṁᕈᚑಽࠍ᷹ቯߒߡᚑಽ⚵ᚑࠍ᳞߼㧘%/$ᴺ %/$%CNVTCPU75# ߦࠃߞߡ⥄േゞឃ᳇ࠟࠬߩነਈࠍ᳞߼ߚ㧚߹ߚ㧘㩀㩨㩇⁁ᳪᨴ‛⾰ߢ޽ࠆ NOx ߦ㑐ߒߡ㧘ᄢ᳇ᳪᨴ᷹ቯゞ㧔ߐࠊ߿߆ภ㧕ߦࠃࠅ㧝ᤨ㑆Ფߩᳪᨴ‛⾰Ớᐲࠍ᷹ቯߒ㧘01Z᜛ᢔ
㩆㩚㨷㩤㨺㩆㨸㩧㩉㩖㩎㧔%CNKPG㧦'2#㧕ߦࠃࠆ⸘▚୯ߣᲧセߔࠆߎߣߢ㧘㜞Ớᐲࠗࡌࡦ࠻߇⿠ߎࠆ᧦ઙߩᛠី෸੍᷹߮ᣇᴺߦ
ߟ޿ߡᬌ⸛ߒߚ㧚㧟㧕⺞ᩏ⎇ⓥߩ㆐ᚑᐲ෸߮ᓧࠄࠇߚᚑᨐ
 Calineߦࠃࠆ⸃ᨆߩ⚿ᨐ㧘NOx 㜞Ớᐲᤨߦ߅޿ߡ㧘㧝ᤨ㑆ᲤߩỚᐲផ⒖ࠍ੍᷹ߔࠆߎߣ߇ߢ߈ߚ㧚߹ߚ㧘NOxߩ〒㔌ᷫ⴮ࠍ੍᷹ߔࠆߎߣߢ㧘㆏〝๟ㄝߩ NOx ỚᐲಽᏓࠍᛠីߔࠆߎߣ߇ߢ߈ߚ㧚  CMBߦࠃࠆ⸃ᨆߩ⚿ᨐ㧘㩍㩨㨲㨺㩈㩨㩣ゞߩነਈࠍᢙ㊂ൻߔࠆߎߣ߇ߢ߈ߚ㧚ౕ૕⊛ߦߪ㧘ᄙ‫⦟ޘ‬ᵺ᳓႐ߦ߅޿ߡ㧘SPM ߇㫌㪾㪆㫄㪊ߣ㜞Ớᐲߢ޽ߞߚ
ᣣߩ䍡䍼䍆䍎䍜䍼䍷ゞ䈱ነਈ䈲㪉㪊㫌㪾㪆㫄㪊䋨㪋㪋䋦䋩䈪䈅䈦䈢䋮䈖䉏䈲ᓟ⢛࿾䈱㪈㪉㫌㪾㪆㫄㪊䈫Ყ䈼䉎䈫䈍䉋䈠㪉୚䈮䈅䈢䉍䋬㆏〝ㄭற䈮䈍䈔䉎䍡䍼䍆䍎䍜䍼䍷ゞ䈎䉌䈱⋥ធ䈱ᓇ㗀䈏␜ໂ䈘䉏䈢䋮䉁䈢䋬ᄙ䇱⦟ᵺ᳓႐䈮䈍䈔䉎࿯ფ䈎䉌䈱ነਈ䈲䋬㪉㪏㫌㪾㪆㫄㪊䋨㪋㪎䋦䋩䈫ᓟ⢛࿾䈱䈠䉏䉋䉍䉅㪐㫌㪾㪆㫄㪊㜞䈒䋬⥄േゞ⿛ⴕ䈮䉋䉎࿯ფ䈱Ꮞ䈐਄䈕䈱ᓇ㗀䈏␜ໂ䈘䉏䈢䋮㧠㧕⋵᳃ߩஜᐽߩ଻ᜬ෶ߪⅣႺߩ଻ో߳ߩ⽸₂ᒰ⹥࿾ၞߢߩ⥄േゞߩ⿛ⴕߣᳪᨴ‛⾰ߩ࿃ᨐ㑐ଥࠍ᣿ࠄ߆ߦߔࠆߎߣߦࠃߞߡ㧘਄⸥ᚻᴺࠍ↪޿ߡઁߩ੤ㅢ㊂ߩᄙ޿㆏〝ߦ߅޿ߡ߽⺞ᩏ෸߮ኻ╷ߩኻ⽎ߣߔࠆߎߣ߇ߢ߈ࠆ㧚߹ߚ㧘ⴕ᡽ߩࠃࠅㄦㅦߥኻᔕߦ᦭ലߢ޽ࠆߣ⠨߃ࠄࠇࠆ㧚㧡㧕⺞ᩏ⎇ⓥ⚿ᨐߩ⁛ഃᕈ㧘ᣂⷙᕈߎࠇ߹ߢ㩍㩨㨲㨺㩈㩨㩣ゞߩᓇ㗀ߦߟ޿ߡ㧘㩍㩨㨲㨺㩈㩨㩣ゞ߆ࠄਥߦឃ಴ߐࠇࠆ☸ሶߢ޽ࠆర⚛⁁὇⚛㧔㧱㧯㧕ߩߺߩỚᐲߢ⹏ଔࠍⴕߞߡ޿ߚ㧚੹࿁㧘ㆡಾߥ⸘▚㩖㨱㩂㩊㨺ࠍㆬቯߔࠆߎߣߢ㧘㩍㩨㨲㨺㩈㩨㩣ゞߩᓇ㗀ࠍᢙ㊂ൻ㧔Ớᐲ෸߮㩔㩩㨺㩈㩧㩎㧕ߔࠆߎߣ߇น⢻ߦߥߞߚ㧚߹ߚ㧘᳇⽎᧦ઙࠍട๧ߒߚᒰ⹥࿾ၞߢߩ㧝ᤨ㑆Ფߩ NOx Ớᐲߩ੍᷹㧘෸߮㆏〝๟ㄝߦ߅ߌࠆ NOxỚᐲಽᏓߩᛠី߇น⢻ߦߥߞߚ㧚㧢㧕ᚑᨐߩᵴ↪⁁ᴫ㧔ᛛⴚ⒖ォ࡮ᵴ↪ߩน⢻ᕈ㧕ᵴ↪ߩน⢻ᕈߦ㑐ߒߡ㧘਄⸥㧠ߩౝኈߩઁ㧘੹࿁ߩ⸘▚ᚻᴺࠍ↪޿ࠆߎߣߢ㧘⥄േゞឃ಴㩀㩨㩇೥ᷫ⋡ᮡߩߚ߼ߩ⥄േゞ೥ᷫบᢙߩ⸳ቯߦ೑↪ߢ߈ࠆߣ⠨߃ࠄࠇࠆ㧚ᛛⴚ⒖ォߦ㑐ߒߡ㧘㩉㩖㩎㧘㩖㨱㩂㩊㨺㧘෸߮ߘߩઁᔅⷐ㩍㩨㨺㩊ࠍᒰ⎇ⓥᚲ߇ឭଏࠍⴕ߁ߎߣߢ㧘ઁߩ಴వᯏ㑐ߢ߽⸘▚߇น⢻ߦߥࠆ㧚 

⚳ੌ㧣⺞ᩏ⎇ⓥ⚳ੌႎ๔ᦠ
⎇ⓥಽ㊁㧦ℂቇ⊛ⷐ࿃ߦࠃࠆⅣႺᓇ㗀ߣߘߩኻ╷ߦ㑐ߔࠆ⎇ⓥ
⺞

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ૐ๟ᵄ㖸ࠍ೙ᓮߔࠆ㒐㖸ოߩ㐿⊒⎇ⓥ⠪ฬ㧔ᚲዻ㧕̪ ٤ශ㧦⎇ⓥઍ⴫⠪٤᧻ᧄḮ↢㧔ᖱႎ▤ℂ⺖㧕
⮮ේᕶม㧔਻Ꮊᄢቇ⧓ⴚᎿቇ⎇ⓥຬ㧕ᧄᐡ㑐ଥㇱ࡮⺖ⅣႺ଻ో⺖⺞ ᩏ ⎇ ⓥ ᦼ 㑆ᐔᚑ㧝㧠 ᐲ⺞ ᩏ ⎇ ⓥ ⒳ ⋡㧝㧚‫ⴕع‬᡽⎇ⓥ‫⺖غ‬㗴⎇ⓥ‫౒ع‬ห⎇ⓥ㧔౒หᯏ㑐ฬ㧦‫غ‬ฃ⸤⎇ⓥ㧔ᆔ⸤ᯏ㑐ฬ㧦㧞㧚‫غ‬ၮ␆⎇ⓥ‫غ‬ᔕ↪⎇ⓥ㧟㧚‫غ‬㊀ὐ⎇ⓥ‫غ‬ផᅑ⎇ⓥ

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‫ع‬㐿⊒⎇ⓥ‫غ‬㧵㧿㧻ផㅴ⎇ⓥ߰ߊ߅߆ᣂ਎♿⸘↹╙㧞ᰴታᣉ⸘↹ᄢ㗄⋡㧦ᓴⅣߦ㈩ᘦߒߚ↢ᵴⅣႺߩ଻ోਛ㗄⋡㧦౏ኂ㒐ᱛኻ╷ߩផㅴዊ㗄⋡㧦⥄േゞ੤ㅢ౏ኂኻ╷ߩផㅴ⑔ጟ⋵ⅣႺ✚วၮᧄ⸘↹㧔㧼㧕̪ⅣႺ㑐ଥߩߺᩇ 㧦 ↢ᵴⅣႺߩ଻ో࠹࡯ࡑ㧦 㕒߆ߥዬ૑ⅣႺߩ⏕଻

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Ԙ ૐ๟ᵄ㖸ԙ น⡬࡮㕖น⡬㖸
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ⷐ㧝㧕⺞ᩏ⎇ⓥߩ⋡⊛෸߮ᔅⷐᕈ
㛍㖸ߩ೙ᓮߦߪ㧘㖸Ḯ஥㧘વ៝⚻〝਄㧘ߐࠄߦฃ㖸஥ߢߩኻ╷߇޽ࠆ㧚⥄േゞ㛍㖸ߦኻߒߡߪ㧘ߎࠇࠄߟߩ႐ᚲ
ߢߩ㛍㖸ૐᷫᛛⴚ߇㐿⊒ߐࠇ㧘᦭ലߥᚻᲑߣߒߡቯ⌕ߒߡ޿ࠆ㧚ߒ߆ߒ㧘๟ᵄᢙ*\એਅߩૐ๟ᵄ㖸ߦ㑐ߒߡߪ㧘㖸Ḯ஥ߢߩ೙ᓮᛛⴚߒ߆⏕┙ߐࠇߡ߅ࠄߕኻ╷߇ਇචಽߢ޽ࠅ㧘ߎߎᢙᐕ⧰ᖱઙᢙ߇Ⴧടߒߡ޿ࠆ㧚ߘߎߢ㧘ᧄ⎇ⓥߢ
ߪૐ๟ᵄ㖸ኻ╷߳ߩነਈࠍ⋡ᜰߒ㧘વ៝⚻〝਄ߢ᦭ലߦᯏ⢻ߔࠆ㒐㖸ოߩ㐿⊒ࠍⴕ߁㧚㧞㧕⺞ᩏ⎇ⓥߩ᭎ⷐ౒㡆ེߩ㗪ㇱߩ૏⟎߿ⓨᵢㇱߩᒻ⁁ߩᎿᄦ㧘ᦝߦߪ㒐㖸ოࠬࡠ࡯ࡊ㕙ߩ᦭ലߥᵴ↪ᣇᴺࠍ⠨᩺ߒ㧘ᐢᏪၞߦ෸߮ᄢ
߈ߥㆤ㖸ലᨐࠍ᦭ߔࠆ㒐㖸ოߩ⸳⸘࡮㐿⊒ࠍⴕ߁㧚⎇ⓥߩታᣉᣇᴺߪએਅߩㅢࠅߢ޽ࠆ㧚Ԙ౒㡆ེࠍ㈩೉᭴ㅧߣߔࠆߥߤലᨐ߇޽ࠆ㈩⟎߿ᒻ⁁ߦߟ޿ߡ㧘㖸㗀ℂ⺰ߦၮߠ޿ߡᰴర㖸႐ࠍᗐቯߒߚᢙ୯ࠪࡒ
ࡘ࡟࡯࡚ࠪࡦࠍⴕ߁㧚ߘߎߢ㧘ዊࠨࠗ࠭ߢߒ߆߽ㆤ㖸ലᨐ߇ᄢ߈޿㒐㖸ოᒻ⁁ࠍ⸳⸘ߔࠆ㧚
ԙ਄⸥!ߦࠃࠅᓧࠄࠇߚ㒐㖸ოᒻ⁁ߦ㑐ߒߡㆤ㖸ലᨐࠍ⏕⹺ߔࠆߚ߼㧘ࠨࠗ࠭ߩ❗ዤᮨဳࠍ૞ᚑߒ㧘ή㗀ቶߦߡᰴర㖸႐ߢߩㆤ㖸㊂ߩ᷹ቯࠍⴕ߁㧚㒐㖸ოࠍ᭴ᚑߔࠆࠦࡦࠢ࡝࡯࠻ߦ㑐ߒߡ㧘ෘߺ߇⭯ߊߒ߆߽ૐ๟ᵄ㖸ࠍㅘㆊߒߥ޿ᚑಽ࡮ෘߐࠍᬌ⸛ߔࠆ㧚㧟㧕⺞ᩏ⎇ⓥߩ㆐ᚑᐲ෸߮ᓧࠄࠇߚᚑᨐ㧔ߢ߈ࠆߛߌᢙ୯ൻߒߡߊߛߐ޿㧕ᮨဳታ㛎ߩᲑ㓏ߦߪ㆐ᚑߢ߈ߥ߆ߞߚ㧚ߒ߆ߒߥ߇ࠄ㧘ᢙ୯ࠪࡒࡘ࡟࡯࡚ࠪࡦࠍᵴ↪ߔࠆߎߣߦࠃࠅࠝࠢ࠲࡯
ࡉࡃࡦ࠼ߩᐢ޿Ꮺၞߦ߅޿ߡF$ᷫ㖸ߔࠆߎߣ߇น⢻ߢ޽ࠆ㒐㖸ოᒻ⁁ࠍᓧࠆߎߣ߇ߢ߈ߚ㧚㧠㧕⋵᳃ߩஜᐽߩ଻ᜬ෶ߪⅣႺߩ଻ో߳ߩ⽸₂ૐ๟ᵄ㖸ߪᔃℂ⊛࡮↢ℂ⊛ߥᓇ㗀㧘଀߃߫⌧⌁㓚ኂ߿㗡∩ߥߤ߇↢ߓࠆ㧚ᧄ⎇ⓥᚑᨐߢᓧࠄࠇߚ㒐㖸ოࠍᵴ↪ߔࠇ߫㧘ߎߩࠃ߁ߥᔃℂ⊛࡮↢ℂ⊛ߥᓇ㗀߇ૐᷫߐࠇ⋵᳃ߩஜᐽߩ଻ᜬ߇น⢻ߣߥࠆ㧚㧡㧕⺞ᩏ⎇ⓥ⚿ᨐߩ⁛ഃᕈ㧘ᣂⷙᕈᧄ⎇ⓥߪ㧘․⸵಴㗿 ․㗿 ࠍⴕߞߚ଻᦭ᛛⴚࠍᵴ↪ߔࠆ㧚ߎࠇߪ㧘㒐㖸ოߩო㕙ࠍ✭߿߆ߥࠬࡠ࡯ࡊ
ߣߒო㕙ࠍ㖸㗀⊛ߦ࠰ࡈ࠻ߣߔࠆߎߣߦࠃࠅ㧘ૐ๟ᵄ㖸ߦኻߒߡ߽᦭ലߥㆤ㖸ലᨐࠍᜬߟ㒐㖸ოࠍ㐿⊒ߔࠆᛛⴚߢ޽
ࠅ਻Ꮊᄢቇߣ౒ห㐿⊒ߒߚ㧚㖸㗀⊛ߦ࠰ࡈ࠻ߥ⴫㕙ࠍታ⃻ߔࠆߚ߼㧘ࡋ࡞ࡓࡎ࡞࠷౒㡆ེߩ㓸ว૕ࠍ㒐㖸ოߩო㕙ߦ㈩⟎ߒ㧘ૐ๟ᵄ㖸ࠍવ៝⚻〝਄ߢᄢ߈ߊૐᷫߔࠆߎߣࠍน⢻ߣߒߚవ┵⊛ᛛⴚߢ޽ࠆ㧚㧢㧕ᚑᨐߩᵴ↪⁁ᴫ㧔ᛛⴚ⒖ォ࡮ᵴ↪ߩน⢻ᕈ㧕ᚑᨐ‛ߪේ᧚ᢱ⾌ߛߌߢ߽㜞ଔ ਁ౞O ߢ޽ࠅ㧘ࠦࠬ࠻ኻലᨐࠍ⠨ᘦߔࠆߣታ↪ൻߪ㔍ߒ޿㧚ߒ߆ߒߥ߇ࠄ㧘ࠦࡦࠢ࡝࡯࠻⵾ຠߦ㑐ߔࠆ㜞♖ᐲߥടᎿᛛⴚࠍ᦭ߔࠆ⑔ጟ⋵ౝߩࠦࡦࠢ࡝࡯࠻ടᎿડᬺߩදജ߇޽ࠇ߫㧘዁᧪⊛ߦߪࠦࠬ࠻ࠍᛥ೙ߒߚታ↪ൻ߇น⢻ߣ್ᢿߒߡ޿ࠆ㧚ߎࠇࠄડᬺ߆ࠄߪ㧘⎇ⓥ⾌߇⺞㆐ߢ߈ࠇ߫දജࠍᓧࠆ⏕⚂ࠍขࠅઃߌ
ߡ޿ߚ߇㧘ᒰ⎇ⓥߦ߅޿ߡᔅⷐߥ⎇ⓥ⾌߇⺞㆐ߢ߈ߥ߆ߞߚߚ߼ታ↪ൻߪታ⃻ߒߥ߆ߞߚ㧚