Anti-PMIS2 antibody, rabbit serum, KO-Validated
Product code73-055Size

100 µlStorage

Store 4℃ for short term For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

Buffer

0.09% sodium azide

PurityRabbit antiserum

ImmunogenSynthetic peptide corresponding to mouse PMIS2 (amino acids 61-80),
CSNWEDAYRNSSRTMWFNML,conjugated with KLH

IsotypeRabbit IgG

Reactivity

Mouse

Special notes

Validation: Knock-Out mouse

Application

1. Western blotting (1/1,000 dilution)
2. Immunohistochemistry (1/1,000 dilution)

Background

PMIS2 might function both as the ultimate factor regulating sperm transport
into the oviducts and in modulating sperm–zona binding.
Molecular mass: 11,040 with 96 amino acids. Likely to be N-glycosylatedExpression: Testis and sperm. Not expressed in other organs

Data Link

UniProtKB Q497Q9 mouse Pmis2.

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 73-055 Anti-PMIS2 antibody, rabbit serum, KO-Validated

Fig. 1. Analysis of PMIS2 protein expression in the lysates of testis and sperm from wild-type (+/+)
and Pmis2 knockout (-/-) mice by western blotting with anti-PMIS2 antibody.
Testes and sperm were lysed in lysis buffer containing 1% Triton-X100 and extracts were prepared assupernatants of lysates after centrifugation. Samples were reacted with anti-PMIS2 antibody at
1/1,000 dilution. The difference in predicted molecular mass (11 kDa) and the band position (18 kDa)of PMIS protein might be due to glycosylation.

Fig.2 Identification of PMIS2 protein in the lysate of mouse sperm by western blotting with antiPMIS2 antibody.
Proteins in the lysate was separated on SDS-PAGE (10~20% gradient gel), electro-blotted to PVDF
membrane and incubated in anti-PMIS2 antibody at 1/1,000 dilution. As the second antibody, goatanti-rabbit IgG antibody conjugated with HRP (Abcam; ab97051) was used at 1/10,000 dilution.The
numbers on the right are positions of molecular size markers in kDa.
The arrow indicates the position of PMIS2 protein.

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Fig.3 Immunohistochemical analysis of paraffin embedded mouse testis tissue with PDILT antibody
Deparaffinization LemosolRA (#122-03991,Wako, Osaka)
Rehydration by ethanol
Antigen retrieval Histo/Zyme (Cat.# k046; Diagnostic BioSystems, California))
Washing

PBST (0.25% triton X-100/PBS-)

Blocking

10 % FBS / PBST

1st antibody

1/1,000 dilution in PBS- 4℃ O/NWashingPBS-

2nd antibody

Goat anti-rabbit IgG Alexa Fluor 488 conjugated,

30 min1,000 dilution, 60 min
WashingPBS-

5 min X 3

DAPI

1.0μg/mL DAPI in TBS

10 minReference: This antibody was described and used in the following publication..
1. Yamaguchi R. et al. (2012) Mice expressing aberrant sperm-specific protein PMIS2 produce
normal-looking but fertilization-incompetent spermatozoa. Mol Biol Cell. 23:2671-9. WB.

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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/