OP Bio Factory Co., ltd.
Company Profile

OP Bio Factory Co., ltd.

Business Model

Value chain of bioresource industry

BioresourcesSam
pling

Libraries

Assay

ActiveCompoundUpstream
Screening

Metabolite
analysis

Breeding

Materialproduction

Midstream

Process improvement

Planning

Product

Mark
eting

Sales

Consu
mers

Downstream
Commercialization

Clients
Pharmaceuticals, Food, Cosmetics
Chemicals, Energy, Environment

1. Library
construction/sales
Bioresource
sampling

Libraryconstruction

・Marine bioresource sampling・Library construction/sales（20,000 samples）

2. Screening
service
Evaluation・in vitro assays
・Compoundpurification/identification

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3. Scale-up
support service
Processimprovement

MaterialProduction

・Improvement of production
process (Breeding, Cultureoptimization)
・Bench-scale production
102１1. Library construction and sales
A Variety of Libraries Fit for Your Purpose
Various Libraries●Extract Libraries
・Actinobacteria
・Filamentous fungi・Lactic acid bacteria
・Yeasts
・Microalgae
・Marine invertebrates
・Plants ・Macroalgae●Fraction Libraries●Compound Libraries●Focused Libraries
・天然中分⼦LC/MS analysis
Diversity analysis
Selection based on chemical diversity
DedupulicationSelected according to a purpose
Focused libraries

Selected isolates
Extract Libraries

16 fractions/extract
Fraction Libraries Compound Libraries

l Libraries consist of selected 20,000 samples.
l Because we launched from a marine survey company, one of ourstrong point is marine bioresource sampling.
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2. Screening service (1) Assay
４Categories of Assays Fit for Your Purposes
For seed exploration, that is, to find active compounds, we undertake
commissioned studies categorized as follows:(1) In-house assays(2) Assay set-up to meet the targets(3) Original assays conducted by out partner companies/universities(4) Assay transfer from clients to our lab
Assay Services
Cell morphology observation assays
Enzyme inhibition/activation assays
Luciferase tests
Antioxidant assays
Various assays associated with genetransferl Multi-genesʼ rapid expression analysis
of multi-samples
l Ca influx assaysl ELISA assays
lllll

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2. Screening service(2) Compound identification
Purification and Structure Determination of Compounds
Structure determination of complex natural compounds require knowledge and experience.
We purify and determine the structure of active compounds using UPLC-MS, GC-MS, and NMR.
Our company has experience in purification and structure elucidation of compounds from avariety of organisms including marine invertebrates, plants, microalgae and microorganisms.
For example, Petrosiol A-E, compounds with neurite outgrowth activity, were found from amarine sponge, in a joint research with Nagoya University.
We identify compounds commissioned by our clients, as well as those from our libraries.

Nuclear magnetic
resonance（600MHz NMR)

バイオテクノロジー研究開発センター共通機器

UPLC-HRMSWATERS UPLC-G2-S Qtof MS
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Petrosiol A-E (Derived from a marine sponge.)
Joint Res. with Nagoya University
Horikawa et al. (2013), Tetrahedron 69 101-106

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3. Support service for scale-up
Support service for commercial scale production
Optimization on fermentation, breeding,extraction, and chemical synthesis forcommercial scale production of microorganisms,microalgae, and compounds derived from them.

Support services●Various development andmanagement support services by ourspecialists●Breeding/culture optimization●Optimization ofextraction/purification methods ofcompounds●Chemical synthesis of compounds
in case of production by fermentationis very challenging.
●Integrated services can also beprovided in collaboration with ourpartner companies.

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Discovery of Pavlova
Our Bioresource Libraries

Microalgae collection (1,000 isolates)• Actinobacteria• Filamentous fungi• Lactic acid bacteria• Yeasts• Microalgae• Macroalgae (Seaweeds)• Plants

Pavlova（OPMS30543）was selected because of high productivity ofcompounds effective for anti-aging, based on metabolite profiling data.
Supported by Okinawa Prefectural Government（知的クラスター形成に向けた研究拠点構築事業）
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Screening using our bioresource(Microorganisms)
Case1: Discovery of Kakeromycin
by using “Ontime” screening method
A new antifungal compoundwith a new core structurefrom a marine actinobacteria.

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Marine sampling × Drug discovery
techniques from natural products“Ontime” screening is the original and innovative screening method for active compound
screening.
The method is developed in combination with marine sampling abilities and techniques and ideasof a researcher who developed Micafungin (Mycamine, Fungard). The high selectivity and shortevaluation terms of the method make screening in high throughput possible at a low cost.
Kakeromycin, a new antifungal compound with a new core structure was found by using thistechnology.
We undertake screening for antibacterial and antifungal agents using the method. The targets
will be expanded to anticancer and antivirus fields in the future.

Common method“Ontime” screening

Extract

Marine samples
from various
regions of Japan

Evaluated with living microbes
Evaluated by extract etc.
Extract/fraction/compoundare applied onto test strains
for evaluation.

Evaluated directly on a specificplate with living cells without
makingextract/fraction/compound.
Copyrights reserved by OP Bio FactoryThroughput
1 million isolates/year

9The case of Kakeromycin
New Antifungal Compound with a New Core Structurel Kakeromycin（SRI-KH001）
Kakeroma Isl., Amami, South-WestIsls., Kagoshima, JapanMarine sediment

Producing strain
Streptomyces sp. A84(undescribed species)

Kakeromycinl Because Kakeromycin requires sea water for its production, it is regardedas a “true” marine actinobacteria-derived compound.
l Patent application pending in 10 countries.
l Development as an candidate for an athlete's foot medicine is ongoing at aventure company spined out from OP Bio (Stage: L1).
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Screening using our bioresource(Microorganisms)
Case2: Discovery of 40551F
by using Molecular Networking Technology
A new active compoundagainst Mycobacterium avium-intracellulare
complex found in a joint researchwith Kitasato University
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Drug discovery using Library datal OPʼs microorganism extract libraries are linked with panel screeningdata.
l By referring to the panel screening data, a screening against
Mycobacterium avium-intracellulare complex, which causes MAC
infection, was conducted in a joint research with Prof. Tomoda(Kitasato Univ.) .
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Screening using Molecular Networking
Extract samples of microorganism cultures (About 6600)
Antibacterial activity against 2 MAC species
Hit samples (652)Activities against Bacillus subtilis and/or mammal cells
Samples showing high selectivity to MAC (41)
LC/MS/MS analysis
MS spectral data of the 41 samples

MS spectral data of 1,000
marine-derived microorganisms(8,000 metabolites)

OPMA40551, OPMA00132 were・Specificity of strains
selected as producing strains of・Chemical diversity
highly novel compounds.
・Dereplication→A new compound, 40551F wasobtained
PCT/JP2018/032048
US Patent US 11,407,742 B2Integrated NetworkDevelopment ongoing at Kitasato Univ. with support fromAMED. In vivo tests are conducted aiming for licensing out.
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13

Screening and product developmentusing our bioresource(Microalgae)Case3: Pavlova, from it discovery toproduct sales
A new microalgal food and cosmetic
material of Haptophyta.

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１

Discovery of Pavlova
Our Bioresource Libraries

Microalgae collection (1,000 isolates)• Actinobacteria• Filamentous fungi• Lactic acid bacteria• Yeasts• Microalgae• Macroalgae (Seaweeds)• Plants

Pavlova（OPMS30543）was selected because of high productivity ofcompounds effective for anti-aging, based on metabolite profiling data.
Supported by Okinawa Prefectural Government（知的クラスター形成に向けた研究拠点構築事業）
Copyrights reserved by OP Bio Factory15

Features of ingredients of Pavlova
No. of nutrient
ingredients
Amino acid score
Proteins(g/100g)
Fat(g/100g)
DHA(mg/100g)
EPA(mg/100g)

Spirulina*

Chlorella*

Euglena*

Pavlova **50 and more

59 and more

59

61 and more

51
65.8
7.5ーー

54
58
8.9ーー

83
50.1
21.3
100300

Carotene: 17

Lutein: +Zeaxanthin: +

94
46.9
23.8
460
3760
Fucoxanthin: 2,000
and more
α-Carotene: 24
Β-Carotene: 105

Phycocyanin: 6,000
Pigments(mg/10Zeaxanthin: 980g)
β-Carotene: 185
Dietary
fiber(g/100g)

9.511.6+7.2

Ca(mg/100g)

379.2

620

532

956ーー+357ーーー

170

GABA(mg/100g)
Hydroxyproline(mg/100g)✓ Many nutrients✓ Good nutrientbalance
Product features ✓ High digestive
absorbability✓ Proteins,
Phycocyanin✓ Many nutrients✓ Good nutrientbalance✓ Many nutrients✓ Good nutrientbalance✓ High digestive
absorbability✓ Paramylon＊ Cited from data on the web, ＊＊Analyzed by Japan Food Research Laboratories,
Copyrights reserved by OP Bio Factory✓ Many nutrients✓ Good nutrientbalance✓ EPA, DHA, Ca,GABA,Hydroxyproline✓✓ Fucoxanthin＋: Detected, but no numerical data available
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Commercial cultivation of Pavlova
Pavlova is cultured with mineral-rich natural sea water under the brightsunshine of Okinawa. To avoid contamination, it is cultured in outdoor
and tube-type photobioreactors, which is first introduced in Japan,
under controlled conditions.
Tube outer diameter: φ65 mm
Total tube length: About 5 km
Total volume: 16 tons

Supported by Cabinet Office, the Government of Japan（国際物流拠点構築推進事業）
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Pavlova Products in the MarketOnline sales at our e-commerce website

Pavlova MCT+ (Supplement)

Served at Cafes and Restaurants

Pavlova Pasta & Dressing
Supervised by BACAR (Italian restaurant)

In-store sales at gourmet/vegan food shops

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