GC Technical Note

Analysis of the Flavor Component Sotolone- Using InertCap Pure-WAXGT001
GL Sciences Inc.

It has been demonstrated that the odor and taste offoods are very closely related, with strong sweeteningof odors and other interactions.

In this application, GC analysis was made for
Sotron reference samples using InertCap Pure WAX
a new WAX-based inert column,. Here we present
the successful results.

Sotolon, a compound responsible for flavor, isdescribed as having an odor of caramel and curry, andis also known to be one of the odor componentscharacteristic of old sake.

Example: Measurement of standard

Structural Formula

100000500 μg/mL

O

80000

Sotolone

OHO

60000

H3CCH340000

Sotolon3-hydroxy-4,5-dimethyl-2(5H)furanone

20000
010Time (min)Structures created using Chemistry 4-D Draw which is provided
By ChemInnovayion Software, Inc.

2010 μg/mL

GC Condition

14000

Sotolone

System:GC - FID

Column: InertCap Pure-WAX
0.25 mm I.D. x 30 m df = 0.25 μm

Column Temp. : 160 ˚C

12000

010Time (min)Carrier Gas: He 100 kPa

Injection: Split Flow 50 mL/min 1 μL240 ˚C20Detection: FID Range 10^0240 ˚CCalibration curve
ソトロン
Sotolone
500000y = 873.45x - 4075.1
R2 = 0.9998

Area
面積値

40000030000020000010000000100

200300濃度(μg/ml)
Concentration(µg/mL)

400

500

600

GL Sciences GC Technical Note

Repeatability of 10 μg/mL injections of Sotlone
Sotolone
6936
7043
7101
68406545
6893
219
3.17

1 st
2 nd
3 rd
4 th
5 thAve.
SDCV (%)

Comparison with a column fromanother manufacturer

40000

Sotolone

InertCap PureWAX

20000

0102030Time (min)Peaks that could not be detected using WAXbased columns from other companies weresuccessfully separated, in terms of peak shape,
repeatability and linearity using InertCap Pure WAX.
Many flavor components are present at very low
concentrations, despite the sensitivity of GC they
can be difficult to measure.
A more accurate sniffing system can be built byusing it in combination with a sniffing GC (OP275).

40000

Other WAX
Column20000

0102030

Time (min)*Sotolon is very unstable. Please inquire if you intend to analyze under different conditions.

InertCap Pure-WAXI.D. (mm)
0.25
0.25
0.32
0.32
0.53
0.53

Length (m)
3060
3060
1530

df (μm)
0.25
0.25
0.25
0.25
1.0
1.0

Max. Operating Temp.(℃)
iso-260-Prog.260
iso-260-Prog.260
iso-260-Prog.260
iso-260-Prog.260
iso-240-Prog.240
iso-240-Prog.240

Cat.No.
1010-68142
1010-68162
1010-68242
1010-68262
1010-68425
1010-68445

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly.
We reserve the right to amend this information or data at any time and without any prior announcement.

GL Sciences, Inc. JapanGL Sciences B.V.

GL Sciences, Inc. USA

GL Sciences (ShangHai) Ltd.

22-1 Nishishinjuku 6-Chome
Shinjuku-ku, Tokyo,163-1130, Japan
Phone: +81-3-5323-6620
Fax: +81-3-5323-6621
Email: world@gls.co.jp
Web: www.glsciences.com

De Sleutel 95652 AS Eindhoven
The Netherlands
Phone: +31 (0)40 254 95 31
Email: info@glsciences.eu
Web: www.glsciences.eu

4733 Torrance Blvd. Suite 255
Torrance, CA 90503
Phone: 310-265-4424
Fax:310-265-4425
Email: info@glsciencesinc.com
Web: www.glsciencesinc.com

Tower B, Room 2003,
Far East International Plaza,NO,317 Xianxia Road,
Changning District.
Shanghai, China P.C. 200032
Phone: +86 (0)21-6278-2272
Email: contact@glsciences.com.cn
Web: www.glsciences.com.cn

International Distributors
Visit our Website at www.glsciences.com/distributors