MANUALTSA™ Research Reagents
Caution: For Laboratory Use. A research chemical for research purposes only.

TSA Plus DIG Kits

Material Provided
Kit contents:

Each TSA Plus DIG kit contains the following 2 components necessary for signal amplification:
DIG Amplification Reagent and 1X Plus Amplification Diluent.

Kit format:

TSA Plus DIG(digoxigenin)

Format*Catalog #Kit Components

50-150 slides

NEL748001KT

1X Plus Amplification Diluent, 1 X 15 mL
DIG Amplification Reagent (2 vials)

250-750 slides

NEL748B001KT

1X Plus Amplification Diluent, 5 X 15 mL
DIG Amplification Reagent (10 vials)

Evaluation size
(25-75 slides)

NEL748E001KT

1X Plus Amplification Diluent, 1 X 7.5 mL
DIG Amplification Reagent (1 vial)*The format of the kit is based on 100 - 300 µL per slide of Amplification Working Solution.

Product Information
Storage:

Store kits in the dark at 4ºC.

Stability:

The components in the kits are stable for a minimum of 3 months under proper storage
conditions.

Application:

TSA Plus DIG reagents are intended for high sensitivity detection in immunohistochemistry(IHC), immunocytochemistry (ICC) and in situ hybridization (ISH) experiments. Final detection
may be fluorescent or chromogenic.

FOR RESEARCH USE ONLY.

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

Safety Note

All reagents are classified as nonhazardous. However, it is strongly recommended to wear
disposable gloves and safety glasses while working. Thorough washing of hands after handling is
also recommended.

Quality Control

We certify that QC results of these reagents meet our quality release criteria.

What is TSA Plus technology?
TSA Plus is based on Tyramide Signal Amplification (TSA™), a technology from PerkinElmer that generally provides 2-3
logs of improved sensitivity while allowing reduced consumption of primary antibodies or probes.
TSA Plus DIG uses horseradish peroxidase (HRP) to catalyze covalent deposition of digoxigenin (DIG) labels directly
adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can thenbe detected using anti-digoxigenin conjugates for fluorescent or bright field microscopy.
The use of TSA Plus reagents results in a significant increase in sensitivity over standard detection methods, while
maintaining specificity and resolution. Moreover, TSA Plus reagents allow drastically reduced consumption of primary
antibody or probe.

Reagents and Materials
Critical Reagents Required but not Supplied HRP-labeled reagent to drive the signal amplification reaction, for example:o SA-HRP for use with biotin-labeled probes or antibodies (cat. no. NEL750001EA)o Anti-digoxigenin-HRP for use with digoxigenin labeled probes or antibodies (cat. no. NEF832001EA)o Anti-fluorescein-HRP for use with fluorescein labeled probes or antibodies (cat. no. NEF710001EA)o HRP-labeled secondary antibody
 Anti-rabbit IgG (goat) HRP (cat. no. NEF812001EA)
 Anti-mouse IgG (goat) HRP (cat. no. NEF822001EA) DMSO (molecular biology or HPLC grade) Buffer components and detergents such as PBS, Triton-X100, Tween-20
 Blocking Reagent (Catalog number FP1012)
 Fluorophore or enzyme labeled anti-digoxigenin for detection of deposited DIG labels.

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

Solutions to prepare
The following buffers and reagents are required for slide preparation and signal amplification
DIG Amplification Reagent Stock Solution
DIG Amplification Reagent is supplied as a solid. Each vial must be reconstituted with 150 µL DMSO to make the DIG
Amplification Reagent Stock Solution. DIG Amplification Reagent Stock Solution is stable for at least 3 months when stored at
4ºC. (Note: DMSO freezes at 4ºC; therefore, thaw Stock Solution before each use).
TNT Wash Buffer
0.1 M TRIS-HCl, pH 7.5
0.15 M NaCl
0.05% Tween®20
Other wash buffers (such as PBS) may be used. Substitution of 0.3% Triton X-100 for the 0.05% Tween-20 is also possible.
Users should validate the use of alternative wash buffers with their own systems.
TNB Blocking Buffer
0.1 M TRIS-HCl, pH 7.5
0.15 M NaCl
0.5% Blocking Reagent (available separately, catalog number FP1020)
Add Blocking Reagent slowly in small increments to buffer while stirring. Heat gradually to 55°C with continuous stirring tocompletely dissolve the Blocking Reagent. (This should take no longer than 30-60 minutes. The solution will appear milky.
Bring to room temperature before using. Aliquot and store at -20°C for long term use.
DIG Amplification Working Solution
Before each procedure, dilute DIG Amplification Reagent Stock Solution1:50 in 1X Plus Amplification Diluent to make DIG
Amplification Working Solution. Approximately 100-300 µL of DIG Amplification Working Solution is required per slide.
Discard any unused portion of DIG Amplification Working Solution.

Recommendations







Do not let slides dry out between steps.
A humidified chamber is recommended for all incubation steps (i.e., a damp paper towel in a covered box).
Drain off as much of the incubation solutions as possible, before the addition of the next solution, to prevent reagent
dilution and uneven staining. Blot area around, but not on, tissue section using a tissue.
Be sure to use enough volume of each reagent to completely cover sections or cells.
Optional: Use of coverslips may reduce reagent evaporation, especially during steps which require long incubation atelevated temperatures (such as probe hybridization). However care must be taken upon removal to prevent damage
to tissues or cells.
First time users should apply TSA Plus DIG to a proven system.

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

Quenching Endogenous Peroxidase
Activation and covalent binding of TSA reagent is catalyzed by peroxidase. Endogenous peroxidase will be a source of
background, and should be quenched if present. Users should establish the need for doing this and optimal methodology
specific to the tissues or cells being stained. Options include: 0.3% H2O2 to 3% H2O2
 Methanol or PBS as diluent for H2O2
 Incubation time of 10 to 60 minutes
For paraffin-embedded tissues, quenching can be done after dewaxing and alcohol rehydration but before the protease
digestion step. For frozen tissue or cell preps, quenching can be done following fixation and before the protease
digestion step. After quenching wash with TNT or 1X PBS buffer for 5 minutes. See Li et al from references section for
more suggestions. Titration of primary and secondary antibodies to find optimal concentration is critical for success withHCA ImagAmp. The presence of excess HRP will favor formation of dimers over label deposition, resulting in decreased
signal and increased background.

TSA-ISH Optimization
Inadequate optimization of probe and HRP conjugate dilution may be a source of high background and reduced signal.
TSA is ideal for detection of targets that have weak signals using standard detection methods. If the signal is strong withstandard detection methods, dilute the probe until the signal begins to disappear and then proceed with TSA
optimization.
Probe Optimization
 Slide 1: use same probe dilution as standard method.
 Slide 2: 5-fold dilution from slide 1
 Slide 3: 2-fold dilution from slide 2 (further dilution may be necessary) Slide 4: negative control (probe omitted)
HRP Conjugate Dilution
 Streptavidin-HRP (catalog number NEL750001EA): 1:250-1:1000 dilution
 Anti-fluorescein-HRP (catalog number NEF710001EA): 1:100-1:500 dilution
 Anti-DNP-HRP (catalog number FP1129): 1:100-1:500 dilution
 Anti-digoxigenin-HRP (catalog number NEF832001EA): 1:100-1:1000 dilution
 HRP reagents from other manufacturers: Begin with recommended range for slide applications. Further dilution
may be needed

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

TSA-ISH Protocol Overview
Standard
nonradiometric
ISH
Blocking Step
Incorporationof HRPTSA Plus
Amplification

Visualization

Quench endogenous peroxidase activity (if needed)
Tissue permeabilization (if needed)
Probe hybridization
Post-hybridization stringency washes↓
Block slides for 30 min. in TNB buffer at room temperature↓
Incubate slides with appropriate HRP reagent (anti-DIG-HRP, SA-HRP, etc.) for 30 min. at room
temperature↓
Wash slides 3X for 5 min. in TNT buffer at room temperature Incubate in DIG Amplification working
solution for 3 to 10 min. at room temperature↓
Wash slides 3X for 5 min. in TNT buffer at room temperature↓
Fluorescent
Chromogenic
Incubate slides inanti-digoxigenin-fluorophore conjugate
for 30 min. at room temperature↓
Wash slides 3X for 5 min. in TNT buffer atroom temperature↓
Counterstain and mount for fluorescencemicroscopy

Incubate slides in anti-digoxigenin-HRP or
anti-digoxigenin-AP
for 30 min. at room temperature↓
Wash slides 3X for 5 min. in TNT buffer atroom temperature↓
Add appropriate chromogen, counterstain andmount for microscopy

Step by Step TSA-ISH Protocol
Step 1: Slide Preparation
Prepare tissues or cells using standard fixation and embedding techniques. Dewax and rehydrate slides according tostandard procedures.
Step 2: Standard Non-radioactive ISH
Follow standard non-radioactive in situ hybridization techniques. Include tissue permeabilization (if needed) andquenching of endogenous peroxidase activity (if needed). Probe hybridization (with digoxigenin, biotin, DNP, or
fluorescein-labeled probes) should be done using at concentration determined in optimization studies followed by posthybridization stringency washes.
NOTE: Always run an unamplified control slide and an amplified negative control slide with each experiment.
Step 3: Blocking Step
Incubate slides with 100-300 µL of TNB Buffer in a humidified chamber for 30-60 minutes at room temperature or at 4ºCovernight.

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

Step 4: Introduction of HRP
Incubate slides for 30 minutes at room temperature or at 4ºC overnight in a humidified chamber with appropriate HRPlabeled reagent using one of the following: For DIG-labeled probes, use 100-300 µL of anti-digoxigenin-HRP (catalog number NEF832001EA) diluted in TNB
Buffer to concentration determined in optimization studies.
 For biotin-labeled probes, use 100-300 µL of SA-HRP (catalog number NEL750001EA) diluted in TNB Buffer toconcentration determined in optimization studies.
 For fluorescein-labeled probes, use 100-300 µL of anti-fluorescein-HRP (catalog number NEF710001EA) dilutedin TNB Buffer to concentration determined in optimization studies.
 For DNP-labeled probes, use 100-300 µL of anti-DNP-HRP (catalog number NEF710001EA) diluted in TNB
Buffer to concentration determined in optimization studies.
Step 5: Wash
Wash the slides 3X for 5 minutes each in TNT Buffer at room temperature with agitation.
Step 6: TSA Plus DIG Amplification
Pipette 100-300 µL of DIG Amplification Working Solution onto each slide. Incubate the slides at room temperature for 3
to 10 minutes.
Step 7: Wash
Wash the slides 3X for 5 minutes each in TNT buffer at room temperature with agitation.
Step 8: Visualization of Deposited DIG
DIG may be visualized for fluorescent or chromogenic detection.
For fluorescent detection, incubate slides with fluorophore labeled anti-DIG as directed by the manufacturer.
For chromogenic detection, incubate slides with HRP or AP labeled anti-DIG followed by detection with an appropriate
chromogenic substrate.

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

TSA-IHC Optimization
Inadequate optimization of primary antibody and HRP conjugate dilution may be a source of high background andreduced signal.
TSA is ideal for detection of targets that have weak signals with standard detection methods. It enables use of much
more dilute antibody solutions for improved specificity. If the signal is strong with standard detection methods, dilute theprimary antibody until the signal begins to disappear and then proceed with TSA optimization.
Primary Antibody Optimization
 Slide 1: use same probe dilution as standard method.
 Slide 2: 5-fold dilution from slide 1
 Slide 3: 5-fold dilution from slide 2
 Slide 4: 5-fold dilution from slide 3 (further dilution may be necessary) Slide 4: negative control (primary antibody omitted)
HRP Conjugate Dilution
 Anti-mouse-HRP (catalog number NEF822001EA): 1:500 to 1:2000 dilution
 Anti-rabbit-HRP (catalog number NEF812001EA): 1:500 to 1:2000 dilution
 Streptavidin-HRP (catalog number NEL750001EA): 1:1250-1:2500 dilution
 Anti-fluorescein-HRP (catalog number NEF710001EA): 1:100-1:500 dilution
 HRP reagents from other manufacturers: Begin with recommended range for slide applications. Further dilution
may be needed

TSA-IHC Protocol Overview
Quench endogenous peroxidase activity (if needed)↓
Block slides for 30 min. in TNB buffer at room temperatureStandard
IHC

Incorporationof HRPTSA Plus
Amplification↓
Incubate slides in primary antibody for 30-60 minutes at room temperature↓
Wash slides 3X for 5 min. in TNT buffer at room temperature↓
Incubate slides in biotinylated secondary
antibody 30-60 min. at room temperature
Incubate slides in HRP labeled secondary
Wash slides 3X for 5 min. in TNT buffer atorantibody for 30 min. at room temperatureroom temperature
Incubate slides in SA-HRP for 30 min. at room
temperature↓
Wash slides 3X for 5 min. in TNT buffer at room temperature↓
Incubate in DIG Amplification working solution for 3 to 10 min. at room temperature↓
Wash slides 3X for 5 min. in TNT buffer at room temperature↓

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

Visualization

Fluorescent
Incubate slides in anti-DIG-fluorophorefor 30 min. at room temperature↓
Wash slides 3X for 5 min. in TNT buffer atroom temperature↓
Counterstain and mount for fluorescencemicroscopy

Chromogenic
Incubate slides in anti-DIG-HRP or anti-DIGAP
for 30 min. at room temperature↓
Wash slides 3X for 5 min. in TNT buffer atroom temperature↓
Add appropriate chromogen, counterstain andmount for microscopy

Step by Step TSA-IHC Protocol
Step 1: Slide Preparation
Prepare tissues or cells for detection with TSA Plus DIG using standard fixation and embedding techniques. Dewax andrehydrate using standard protocols. Quench endogenous peroxidase activity if necessary.
NOTE: Always run an unamplified control slide and an amplified negative control slide with each experiment.
Step 2: Blocking Step
Incubate slides with 100-300 µL of TNB Buffer in a humidified chamber for 30-60 minutes at room temperature or at 4ºCovernight. (Note: PBS may be substituted for TN buffer.)
Step 3: Primary Antibody Incubation
Drain off the TNB Buffer and apply 100-300 µL of primary antibody, diluted in TNB Buffer. Incubate the primary antibody
preparation per the manufacturer's instructions regarding incubation time and temperature requirements. Useconcentration determined in optimization studies.
Step 4: Wash
Wash the slides 3X for 5 minutes each in TNT Buffer at room temperature with agitation.
Step 5: Introduction of HRP
Incubate slides with HRP by doing one of the following: 100-300 µL of HRP labeled secondary antibody diluted in TNB Buffer.
 100-300 µL of biotinylated secondary antibody diluted in TNB Buffer. Incubate 30-60 minutes in a humidified
chamber. Wash the slides for 3 X 5 minutes TNT buffer at room temperature with agitation. Follow by 100-300 µL
of SA-HRP diluted in TNB Buffer. Use SA-HRP at 1:2000 if using PerkinElmer Cat. # NEL750001EA. Whenusing alternative suppliers, reagents should be optimized for use with TSA starting with manufacturer's
recommended dilu¬tions. Incubate slides in a humidified chamber for 30-60 minutes at room temperature or at
4ºC overnight.
Step 6: Wash
Wash the slides 3X for 5 minutes each in TNT Buffer at room temperature with agitation.
Step 7: TSA Plus DIG Amplification
Pipette 100-300 µL of DIG Amplification Working Solution onto each slide. Incubate the slides at room temperature for 3
to 10 minutes.
Step 8: Wash
Wash the slides 3X for 5 minutes each in TNT Buffer at room temperature with agitation.
PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

Step 9: Visualization of Deposited DIG
DIG may be visualized for fluorescent or chromogenic detection.
For fluorescent detection, incubate slides with fluorophore labeled anti-DIG as directed by the manufacturer.
For chromogenic detection, incubate slides with HRP or AP labeled anti-DIG followed by detection with an appropriate
chromogenic substrate.

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

Troubleshooting
Technical Support Resources Assay Support Knowledge Base: Email: Telephoneo USA toll-freeo EU toll-freeo Finland toll-freeo China toll-free

www.perkinelmer.com/askTSA
global.techsupport@perkinelmer.com
800-762-4000
00800 33 29 0000
999 800 33 29 0000
800 820 5046

ISH Troubleshooting
PROBLEM

REMEDY

Low Signal






Optimize probe concentration.
Titer HRP conjugate to determine optimum concentration for signal amplification.
Add tissue permeabilization step to facilitate penetration of reagents.
Lengthen incubation time for DIG Amplification Working Solution.

Excess Signal






Decrease concentration of HRP conjugate introduced prior to amplification.
Decrease probe concentration.
Decrease DIG Amplification Reagent incubation time.
Decrease concentration of anti-DIG-enzyme conjugate used for visualization.

High
Background









Decrease concentration of HRP conjugate.
Decrease probe concentration.
Shorten chromogenic development time.
Lengthen endogenous peroxide quenching step.
Filter buffers.
Increase number and/or length of washes.
Nonqualified or contaminated blocking reagent used. Use PerkinElmer Blocking Reagent(FP1020).

IHC Troubleshooting
PROBLEM

REMEDY

Low SignalTiter primary and/or secondary antibodies to determine optimum concentration for signal
amplification
Lengthen incubation time for DIG Amplification Working Solution.
Use antigen retrieval techniques to unmask the target.

Excess SignalDecrease concentration of primary and/or secondary antibody or HRP conjugates.
Decrease DIG Amplification Working Solution incubation time.
Decrease concentration of anti-DIG conjugates used for visualization.

High
Background







Filter buffers
Decrease concentration of primary and/or secondary antibody or HRP conjugates.
Lengthen endogenous peroxide quenching step.
Increase number and/or length of washes. Shorten chromogenic development time.
Nonqualified or contaminated blocking reagent used. Use PerkinElmer Blocking Reagent(FP1020).

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

Selected References
A quantitative evaluation of peroxidase inhibitors for tyramide signal amplification mediated cytochemistry andhistochemistry
G. Li, S. Amin, N. N. Okuhama, G. Liao, L. A. Mingle
Histochem Cell Biol (2006)
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1713225
Dual Fluorescent In Situ Hybridization and Immunohistochemical Detection with Tyramide Signal Amplification
Aliya U. Zaidi, Hideki Enomoto, Jeffrey Milbrandt, and Kevin A. RothJ Histochem Cytochem. (2000)
http://www.ncbi.nlm.nih.gov/pubmed/10990490
Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
Tim Brend, Scott A. Holley
J Vis Exp. (2009)http://www.ncbi.nlm.nih.gov/pubmed/19322135
Genomic Anatomy of the Hippocampus
Carol L. Thompson, Sayan D. Pathak, Andreas Jeromin, Lydia L. Ng, Cameron R. MacPherson, Marty T. Mortrud, Allison
Cusick, Zackery L. Riley, Susan M. Sunkin, Amy Bernard, Ralph B. Puchalski, Fred H. Gage, Allan R. Jones, Vladimir B.
Bajic, Michael J. Hawrylycz and Ed S. Lein
Neuron. (2008)
http://www.ncbi.nlm.nih.gov/sites/entrez/19109908?dopt=Abstract&holding=f1000,f1000m,isrctn
Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acidprobes and tyramide signal amplification
Asli N Silahtaroglu, Dorrit Nolting, Lars Dyrskjøt, Eugene Berezikov, Morten Møller, Niels Tommerup & Sakari Kauppinen
Nat Protoc. (2007)
http://www.ncbi.nlm.nih.gov/pubmed/17947994
Introduction of Tyramide Signal Amplification (TSA) to Pre-embedding Nanogold–Silver Staining at the Electron
Microscopic Level
Seung-won Lee, Song Eun Lee, Seong Hyuk Ko, Eun Kyoung Hong, Kwang Il Nam, Kei-ichiro Nakamura, Shuhei
Imayama, Yeong-Joon Park, Kyu Youn Ahn, Choon Sang Bae, Baik Yoon Kim, and Sung Sik ParkJ Histochem Cytochem. (2005)
http://www.ncbi.nlm.nih.gov/pubmed/15684338

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

TSA reagents and related methods are covered by U.S. Patents 5,863,748, 6,372,937, 6,593,100, and 6,617,125, related patent applications, and international equivalents
thereof.
For Research use only. This product is distributed and sold for research purposes only by the end-user in the research market, and, to that extent, by purchasing this productthe end-user is granted a limited license to use this product for research use only. This product is not intended for diagnostic or therapeutic use and no license or right isgranted for use of this product for diagnostic or therapeutic purposes. Purchase does not include or carry any right or license to use, develop or otherwise exploit this productcommercially. Any commercial use, development or exploitation of this product without the express prior written authorization of PerkinElmer is strictly prohibited and may
constitute infringement of the intellectual property rights of PerkinElmer under the aforementioned patents.
TSA is a registered trademark of PerkinElmer. Other trademarks are property of their respective owners.

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.