InertCore Plus C18

The High Performance Core-shell Column For UHPLC/HPLC・Base Material：Core-shell type silica gel

・End-capping：Yes

・Particle Size：2.6 μm

・Carbon Loading：15 %・Solid Core：2.0 μm

・USP Code：L1

・Surface Area：200 m /g

・pH Range：1 - 10

・Pore Size：90 Å (9 nm)

・Max Pressure：100 MPa (2.1 mm I.D.)

2・Functional Group：Octadecyl

60 MPa (3.0 mm I.D., 4.6 mm I.D.)

The manufacturing process is strictly controlled to minimize differences
between and to ensure quality and supply stadility.

Core-Shell Technology for the highest theoretical plates.
The molecules of the target analytes pass through the fine pores in the particles in the packingmaterial. Core-shell particles are formed around a solid non-porous core, resulting in less diffusion
inside the fine pore structure. This differs from the more traditional fully porous particle.
When using a core-shell column, the distribution of molecules in the peak is narrower when thetarget compound is eluted from the column, resulting in a greater number of theoretical plates.

Core-Shell type packing material
Non-porous core

Porous shell

Conventional fully porous packing materialFully Porous particle●The effect of multiple-path diffusion is significantly reduced by improving the particle size distribution homogeneity tothe highest achievable limits.
●A 2.6μm core-shell packing material achieves a comparable number of theoretical plates as a Sub 2μm fully porous
packing material, but with the same back-pressure as a column using 3μm fully porous packing material.
●The theoretical plates are improved by reducing diffusion inside the particle.

Small peak-widthSmall peak-width
broadening
broadening

2Peak-width

Peak-width

broadeningbroadening

Comparison with Products from OtherSuppliers
The InertCore Plus C18 columns are subjected to rigorous internal quality control from bare silica
manufacturing to inspection, resulting in superior reproducibility due to shell layer homogeneity.

SEM imageInertCore Plus C18
Surface

Cross-section

Shell layer ishomogeneous.

Core-shell columns from other suppliers
Surface

Cross-section

Shell layer is nonhomogeneous

Compare with other core-shell brand columns, InertCore Plus C18 delivery extremely stable batch-to-batchperformance.

Particle size distribution
InertCore Plus C18

Competitor

10109988The variation inparticle size
distribution
between the lots issmall.

6543

7
Volume (%)

Volume (%)

7543221100.5

124Particle diameter (µm)

816

The variationin particle size
distribution
between lots islarge.

600.5

124Particle diameter (µm)

8163Superior Reproducibility
Variation in
retention
power
between lots
is small.

InertCore Plus C18
23
Lot A

45

6

1
1.0

23
Lot B

2.0

61
1.0

2.0

Conditions

62.0

345

6

2.0

1.0

Time (min)44562.0Time (min)3.0

5

R: 2. Toluene	
3. Ethylbenzene
4. Propylbenzene
5. n-Butylbenzene
6. n-Amylbenzene— CH3— CH2CH3— CH2CH2CH3— CH2CH2CH2CH3— CH2CH2CH2CH2CH3

62.0

1.0

Time (min)InertCore Plus C18
Variation ofretention power
and peak shape
between lots issmall.

213

2

1.0

1

Variation ofretention power
and peak shape
between lots islarge.

21.0

2.0

Time (min)

13

3.0

Competitor

31

3.0

1
1.0

Lot B

3.0

Column Size ：2.6 μm, 100 × 2.1 mm I.D.
: CH3OH/H2O = 80/20, v/v
EluentFlow Rate: 0.4 mL/min
Col. Temp.
: 40 ℃
Detection: UV 254 nm
Injection Vol. : 0.2 μL
Sample: 1. Uracil 2. Toluene 3. Ethylbenzene
4. Propylbenzene 5. n-Butylbenzene
6. n-Amylbenzene

331Lot A

5Time (min)

22

Lot C

4１ Hydrophobic retention test

13.0

Time (min)31.025Competitor

1

3.0Time (min)422.0Time (min)231.02.0
2 1.0 Time (min)2 Strongly basic compounds
adsorption test

ConditionsColumn Size ：2.6 μm, 100 × 2.1 mm I.D.
: A) CH3CN
EluentB) 25 mM K2HPO4 (pH 7.0, KH2PO4)
A/B = 40/60, v/v: 0.4 mL/min
Flow Rate: 40 ℃
Col. Temp.
: UV 220 nm
Detection
Injection Vol. : 0.2 μL: 1. Uracil 2. Berberine chloride
Sample3. Dextromethorphan

2.0Time (min)2Lot C

113

3

1:Uracil(Neutrality)

1.04

2.0

Time (min)

1.0

2.0

Time (min)2:Berberine chloride

3:Dextromethorphan(Basicity)(Basicity)

High Durability
InertCore Plus C18 has high durability demonstrating that the performance for retention time,
theoretical plate and symmetry factor is maintained even after 2000 continuous injections.

0.08

Conditions
Column Size : 2.6 μm, 100 × 4.6 mmI.D.
Eluent: CH3CN/0.1 % H3PO4 in H2O = 40/60, v/v
Flow Rate: 1.0 mL/min
Col Temp.
: 40 ℃
Detection: UV 254 nm
Inj. Vol.
: 10 μL
Sample: 1. Uracil
1 mg/L  
2. Benzoic acid20 mg/L
3. Acetophenone   	 5 mg/L

0.04

210.00

0.02

Volts

0.06

3Performance is0.0

1.0Time (min)maintained even after2.0

2000 injections.

Variation (%)

Retention time

3. Acetophenone

Variation (%)

2. Benzoic acid

Injection number

Variation (%)

Theoretical plates

Variation (%)

Injection number

Injection number

Variation (%)

Symmetry factor

Variation (%)

Injection number

Injection number

Injection number

5Suitable for Both General Purpose
HPLC and UHPLCThe results of analyses performed with the InertCore Plus C18 on two different HPLC systems, under thesame conditions are shown. Peak shapes are excellent in both cases and equivalent chromatograms wereobtained.

When UHPLC is used5

Pressure: 22.0 MPa (including all plumbing, etc.)
Degree of separation between peak 2 and peak 3: 3.65

0.20

32Volts
0.1040.00

10246810Time (min)1214When general purpose HPLC is used

16Pressure: 19.0 MPa (including all plumbing, etc.)
Degree of separation between peak 2 and peak 3: 3.29

52003mAU
100

2Conditions
40

102468
10Time (min)121416Column Size : 2.6 μm, 150 × 4.6 mm I.D.
Eluent: A) 10 mM KH2PO4 in H2O(pH 2.0, H3PO4)B) CH3CN
Flow Rate: 1.0 mL/min
Col.Temp.
: 40 ℃
Detection: PDA
Injection Vol. : 2 μL
Sample: 1. Benzoic acid		 2. Ethyl p-Hydroxybenzoate		 3. Acetophenone		 4. Amitriptyline
5. Indomethacin		 (100 mg/L in 50 % MeOH)＊To eliminate the effect of system volume, plumbing size, etc. A general purpose HPLC system is optimized.

6Ordering Information
Analysis column
Description

InertCore Plus
C18Particle Size(μm)

2.6Length /Inner Diameter (mm)

2.1

3.0

4.6

505020-17510

5020-17515

5020-17520

75

5020-17513

5020-17518

5020-17523

1005020-17511

5020-17516

5020-17521

1505020-17512

5020-17517

5020-17522

Inner Diameter (mm)Length (mm)Cat. No

2.1205020-17506

3.0205020-17507

4.6205020-17508

Note) The type of fitting connections used is Parker type (UP type).

Guard column
Description

Particle Size(μm)

InertCore Plus
C182.6Note) The type of fitting connections used is Parker type (UP type).

7

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly.
We reserve the right to amend this information or data at any time and without any prior announcement.

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Published in Japan, July 21, 2022(PDF)
20220721 ED