Anti-Vaccinia virus L1

antibody, mouse monoclonal (NP2),(cross-reacts with Monkeypox virus)
Product code65-038Size50 µg

Storage-20℃Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

ImmunogenLysate of Vaccinia virus-infected cells

Isotype

Mouse IgG1κ

Reactivity

Nucleoprotein (L1) of Vaccinia virus, Monkeypox virus, Cowpox virus andEctromelia virus

Special notes

N/AApplication

1. Western blotting: 1/400-800 (Fig.1)
2. Immunofluorescence: 1/400 (Fig.2)

Background

Variola virus (VAV), vaccinia virus (VV), cowpox virus (CPV) and Monkeypox(MPV) belong to the genus Orthopoxvirus of the family Poxviridae. The VAV andMPV cause serious, contagious, and sometimes fatal disease. Therefore,
confirmation of these outbreaks requires rapid and reliable detection anddiagnosis.
Several major antigens have been shown to be induced in cells infected with
poxviruses, i.e., the nucleoprotein (NP) antigen, neutralization (NT)-associatedantigen. The NP (also called L1 or L1R) is a so-called common antigen thatshows cross-reactivity with cells infected with almost all poxviruses. The L1protein, a component of the mature virion membrane, is required for virus entry
into host cells and is a target for neutralizing antibody. The L1 is a 250-aminoacid polypeptide (with molecular weight of 28kDa). Although L1 has a Cterminal hydrophobic segment embedded in the viral membrane, the 185residue (22kDa), disulfide-bonded ectodomain is located in the cytoplasm before
lysis of the host cells.

Data Link

UniProKB: Q71TT2＿9POXVPlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 2

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: Anti-Vaccinia virus L1

antibody, mouse monoclonal (NP2), (cross-reacts withMonkeypox virus)

Fig.1. SDS-PAGE (a) of the recombinant VV L1 protein and Western blot (b) using NP2 antibody.
The recombinant VV L1 protein (185-residues, 22kDa) and the lysates of VV- or CPV-infected RK13cells were applied to SDS-PAGE. (1) Marker, (2) recombinant L1 protein, (3) NP40 lysate of VV Listerstrain-, (4) NP40 lysate of CPV LB strain-infected RK13 cells. The NP2 antibody was used at 1/400dilution. The HRP-conjugated goat anti-mouse IgG (Abcam) was used at 1/3,000 as the second
antibody. A 28kDa band was identified as VV L1 protein.

Fig.2. Immunofluorescence staining of VV-, CPV-, and MPV-infected cells.
Virus-infected and uninfected cells on a slide glass were fixed with acetone. (a) VV Lister strain-, (b)VV Ikeda strain-, (c) CPV LB strain-, (d) uninfected-RK13 cells, and (e) MPV r599 strain-, (f) MPV
Liberia strain-infected Vero E6 cells. The NP2 antibody was used at 1/400 dilution. The FITCconjugated goat anti-mouse IgG (Abcam) was used at 1/3,000 as the second antibody.
Reference: This antibody has not yet been used in publication.
Related products:
65-039 Anti-Vaccinia virus L1 antibody, mouse monoclonal (P3), neutralizing
2 / 2

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/