In Vivo Imaging Reagents

Bioluminescent Reagents and Fluorescent Probes, Dyes, and Labels

Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

INTRODUCTION

More Insightful Research Results

INVISense™ Fluorescent Agents
 Activatable Probes Targeted Probes Vascular Probes
 DyesOur comprehensive range of bioluminescent and fluorescent imaging agents provides researchers with the necessary
tools to better understand early disease-related biological changes, track disease progression, help guide the drug
discovery process, and evaluate efficacy and safety of drug candidates. All of our optical imaging reagents, probes,
labels, and dyes have been optimized and validated in a broad range of imaging applications using our industryleading IVIS® imaging platform.

 Cell Labeling Dyes
 Panels Agents by Disease

IVISbrite™ Bioluminescent Reagents
 Substrates
 Tumor Cell Lines Lentiviral Particles
 Optical Reporter Bacteria

In Vivo Imaging Instruments
Solutions and Support
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MMP and cathepsin activity in 4T1 tumors using IVISense™
MMP 680 and IVISense Pan Cathepsin 750

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Fluorescence imaging of cathepsin activity in antibody induced
arthritis using IVISense Pan Cathepsin 750 probe

Bioluminescence imaging of IVISbrite™ Red F-luc transduced
A549 lung tumor cells

Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IVISense™ FLUORESCENT AGENTS

INVISense™ Fluorescent Agents

Detect, Understand, and Characterize
Disease Earlier
PerkinElmer’s comprehensive suite of validated fluorescent imaging agents,
designed for use in your in vivo research, enables unmatched imaging ofa broad range of disease-related biomarkers and protease activity. Our
IVISense fluorescent probes, dyes, and cell labeling dyes are optimized foruse in our IVIS optical in vivo imaging systems, as well as other fluorescencemicroscopy systems and many in vitro and cell-based systems.

The FAST platform represents the next generation of agents from PerkinElmer.
Utilizing a novel small molecule design, the FAST agents offer improved specificity,
accelerated activation profile, and earlier imaging timepoints.

Target protease
Fluorescentdyes quenchedby proximity

VivoTag

Linker3|

Protease-specific peptide

Dyefluoresces

Dyefluoresces

VivoTag

VivoTag

VivoTag

LinkerLinker

www.perkinelmer.com

Peptide cleavedby protease

Linker Targeted Probes Vascular Probes
 Dyes Cell Labeling Dyes

PROBE

PRODUCT DESCRIPTIONIVISense Cat B 680 FAST

Selective imaging of cathepsin B proteinases(Cat B). Optically silent in the inactivatedstate, becoming highly fluorescent when
activated.

NEV11112

Imaging of cathepsin K (Cat K) activity inoncology applications involving metastasis
to the bone as well as a broad range ofbone applications, including bone loss,
tumor-induced osteolysis, and bone changes
following arthritis.

NEV11000

Imaging of metalloproteinase (MMP)
activity is involved in many disease-related
phenomena, including cancer propagation,
invasion and metastasis, rheumatoid arthritis,
and areas of cardiovascular disease.

NEV10100

IVISense Neutrophil Elastase
680 FAST

Fluorescent neutrophil elastase-activatableagent that is optically silent upon injection andproduces a fluorescent signal after cleavage
by elastase produced by neutrophil cells.

NEV11169

IVISense Pan Cathepsin 680(formerly ProSense 680)

Versatile imaging of changes in cathepsinbased protease activity as seen in a numberof pathological states and disease-related
events, including rheumatoid arthritis,
cancer, atherosclerosis, angiogenesis, andcardiovascular disease.

NEV10003

IVISense Cat B 750 FAST
IVISense Cat K 680 FAST

Activatable In Vivo Imaging Fluorescent Probes
Activatable agents are optically silent upon injection, but are activated in vivo throughcleavage by specific protease biomarkers of disease. The probes are designed totarget and read out disease-related molecular activities with high signal-to-noise ratios
at the biological target.

 Activatable Probes

IVISense MMP 645 FAST
IVISense MMP 680
IVISense MMP 750 FAST

IVISense Pan Cathepsin 750

CAT. NUMBER

 Panels Agents by Disease

NEV11098

IVISbrite™ Bioluminescent Reagents
 Substrates
 Tumor Cell Lines Lentiviral Particles
 Optical Reporter Bacteria

NEV10126
NEV10168

NEV10001EX

IVISense Pan Cathepsin
750 FAST

FAST version of ProSense, with fasterkinetics and a broader imaging window.

NEV11171

IVISense Renin Receptor
680 FAST

Imaging of renin-angiotensin pathway
associated with hypertension, and kidney andcardiovascular disease.

NEV11079

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Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IVISense™ FLUORESCENT AGENTS

INVISense™ Fluorescent Agents
 Activatable Probes Targeted Probes

Targeted In Vivo Imaging
Fluorescent Probes
Optimized in vivo imaging agents that actively
target and bind to specific biomarkers. These
probes are designed to target key biology,
such as cell surface receptor upregulation orbone turnover, and accumulate directly at the
local site with high specificity.

PROBE
IVISense Osteo 680

Small moleculelabeled withfluorescent dye
Binds tomembrane-boundreceptor

IVISense Osteo 750

IVISense Tomato
Lectin 680

PROBE

PRODUCT DESCRIPTIONIVISense
Annexin-V 750

In vivo targeting of membrane-bound phosphatidylserine
exposed during the early stages of apoptosis.

NEV11053

IVISense Bombesin
Receptor 680

Target and quantify up-regulation of bombesin receptors(BBR) in vivo associated with tumor proliferation. These
receptors are also over-expressed in a variety of cancers.

NEV10090

Highly specific and sensitive in the detection of folate
receptor proteins. Can be used to closely monitor andquantitate tumor growth and metabolism.

NEV10040

IVISense Hypoxia
CA IX 680

Detects the tumor cell surface expression of carbonic
anhydrase 9 (CA IX) protein, which increases in hypoxic
regions within many tumors.

NEV11070

IVISense Integrin
Receptor 680

Targets integrin αvβ3 expressed in oncology,
atherosclerosis, and angiogenesis disease models.

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IVISense Bacterial 750 Probe in
RediJect™ Solution (1 vial)
IVISense Bacterial Probe 750 in
RediJect Solution (4 vials)
IVISense Bacterial 750 Control
Dye in RediJect Solution (1 vial)
IVISense 2-DG 750 Probe in
RediJect Solution (1 vial)
IVISense 2-DG 750 Probe in
RediJect Solution (4 vials)

NEV10640

IVISense Integrin
Receptor 750

4CAT. NUMBER

IVISense Folate
Receptor 680

NEV10645
NEV10873

Optimized imaging of bone turnover throughbinding of hydroxyapatite.

IVISense Osteo 800

IVISense Transferrin Receptor 750

IVISense Integrin
Receptor 645

PRODUCT DESCRIPTIONIVISense 2-DG 750 Control Dye
in RediJect Solution (1 vial)

CAT. NUMBER

 Dyes

NEV10020EX

 Cell Labeling Dyes

NEV10053EX

 Panels

NEV11105

 Agents by Disease

NIR-labeled tomato lectin protein thathas high binding affinity for glycoprotein
N-acetylglucosamines on the surface ofvascular endothelial cells. Use for vascularmapping in vivo.

NEV10060

NIR-labeled transferrin detects transferrin
receptor upregulation associated with theincreased cell metabolic need for iron in cancer
and inflammatory cells. Also detects normal ironmetabolism in the liver.

NEV10091

IVISbrite™ Bioluminescent Reagents
 Substrates
 Tumor Cell Lines Lentiviral Particles
 Optical Reporter Bacteria

133397
NIR-targeted probe for non-invasive detectionof bacterial infections in vivo.

 Vascular Probes

133398In Vivo Imaging Instruments
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Non-reactive control dye for RediJect
Bacterial Detection Probe.

133399760561

NIR-targeted probe for non-invasive imaging
of glucose uptake in vivo.

Non-reactive control dye for RediJect 2-DG
750 probe.

760562760567

Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IVISense™ FLUORESCENT AGENTS

INVISense™ Fluorescent Agents
 Activatable Probes Targeted Probes

Vascular In Vivo Imaging Fluorescent Probes Vascular Probes

PerkinElmer’s vascular and physiological agents are a range of highly fluorescent in vivo imaging molecules designed to remain highly stable and localized in the anatomy forvarious periods of time to enable imaging of disease physiology, vasculature, vascular permeability, and angiogenesis.

 Cell Labeling Dyes

 Dyes Panels Agents by Disease

PROBE

PRODUCT DESCRIPTION

CAT. NUMBER

PROBE

PRODUCT DESCRIPTIONIVISense Vascular 680

Imaging of vascularity, perfusion, and vascularpermeability. Remains localized in vasculature for 0-4
h; accumulates in tumors and arthritic joints at 24 h.

NEV10054EX

IVISense GFR 680

NIR-fluorescent imaging agent to non-invasively
determine glomerular filtration rate (GFR) in vivo
in models of kidney disease, dysfunction, and drug
toxicity.

NEV30000

Fluorescent nanoparticles for imaging vascularity,
perfusion and vascular permeability; longpharmacokinetic profile.

NEV10149

NEV10116

Imaging to monitor gastric emptying and the impact ofvarious drugs on gastric motility; may also be used as
an anatomical marker for the stomach.

NEV11121

Blood pooling fluorescent probe for imaging circulation,
blood vessels, vasculature, vascular leak, including thatassociated with early oncologic and ophthalmologic
lesions. This agent has a short pharmacokinetic profile
with bladder clearance, and binds to albumin in bloodfor a modestly extended (30m-1h) circulation half-life.

IVISense Vascular 750
IVISense Vascular NP 680
IVISense Vascular NP 750
IVISense
Gastrointestinal 750
IVISense Acute
Vascular 680 (1 mg)
IVISense Acute
Vascular 680 (5 mg)
IVISense Acute
Vascular 750 (1 mg)

NEV10011EX

NEV10150

IVISense Edema 680

NEV10117
Fluorescent probes that enable imaging andquantitation of acute vascular permeability
increase, bladder clearance via intravital ureter
imaging, or incorporation into liposomes for drugdelivery monitoring.

IVISense Acute
Vascular 750 (5 mg)|

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IVISbrite™ Bioluminescent Reagents
 Substrates
 Tumor Cell Lines Lentiviral Particles
 Optical Reporter Bacteria

In Vivo Imaging Instruments
Solutions and Support

NEV10130

Contact Us

NEV10118
NEV10177

Measure Kidney Function
Non-Invasively In Vivo

5CAT. NUMBER

Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IVISense™ FLUORESCENT AGENTS

INVISense™ Fluorescent Agents
 Activatable Probes Targeted Probes

Vascular In Vivo Imaging Fluorescent Probes Vascular Probes

X

PerkinElmer’s vascular and physiological agents are a range of highly fluorescent in vivo imaging molecules designed to remain highly stable and localized in the anatomy forvarious periods of time to enable imaging of disease physiology, vasculature, vascular permeability, and angiogenesis.

PROBE

PRODUCT DESCRIPTION

CAT. NUMBER

IVISense Vascular 680

Imaging of vascularity, perfusion, and vascularpermeability. Remains localized in vasculature for 0-4
h; accumulates in tumors and arthritic joints at 24 h.

NEV10054EX

Fluorescent nanoparticles for imaging vascularity,
perfusion and vascular permeability; longpharmacokinetic profile.

NEV10149

Imaging to monitor gastric emptying and the impact ofvarious drugs on gastric motility; may also be used as
an anatomical marker for the stomach.

NEV11121

IVISense Vascular 750
IVISense Vascular NP 680
IVISense Vascular NP 750
IVISense
Gastrointestinal 750
IVISense Acute
Vascular 680 (1 mg)
IVISense Acute
Vascular 680 (5 mg)
IVISense Acute
Vascular 750 (1 mg)

NEV10011EX

NEV10150

NEV10117
Fluorescent probes that enable imaging andquantitation of acute vascular permeability
increase, bladder clearance via intravital ureter
imaging, or incorporation into liposomes for drugdelivery monitoring.

IVISense Acute
Vascular 750 (5 mg)

NEV10130
NEV10118
NEV10177

Measure Kidney Function
Non-Invasively
In DESCRIPTION
Vivo
PROBE
PRODUCT

Glomerular
filtration rate NIR-fluorescent(GFR) is the imaging
gold standard
in kidney
IVISense GFR 680
agent to non-invasively
filtration
rate (GFR) in ofvivo
function assessment anddetermine
is usedglomerular
to determine
progression
in models of kidney disease, dysfunction, and drug
kidney disease and drug-induced kidney toxicity. IVISense GFR
toxicity.
680 is a near infrared (NIR) fluorescent-labeled form of inulin
IVISense
Edemaregion
680
Blood pooling
fluorescent probe
forhighimaging
circulation,ina spectral
providinglow backgroundandtissueblood vessels,
vasculature,leak, including thatpenetration (ex/em = 670/685nm) forin vivovascularapplications.

associated with early oncologic and ophthalmologic
lesions. This agent has a short pharmacokinetic profile
Fluorescence molecular tomographic (FMT) imaging of thewith bladder clearance, and binds to albumin in blood
heart was used to detectfor
andquantify
blood(30m-1h)
levelscirculationof IVISensea modestly
extendedhalf-life.

GFR 680 at multiple time points, providing the necessary
data to calculate the clearance rates in individual animals.
Following an intravenous bolus of IVISense GFR 680 in SKH1E mice, FMT images were acquired at 1, 5, 15, 30, and 45
minutes post-injection GFR-Vivo 680, and in combination
with FMT heart imaging, provide a non-invasive fluorescentimaging approach to generate consistent GFR measurements
in models of kidney disease, dysfunction, and drug toxicity.

Measure Kidney Function
Non-Invasively In Vivo

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 Dyes Cell Labeling Dyes
 Panels Agents by Disease

CAT. NUMBER
NEV30000

IVISbrite™ Bioluminescent Reagents
 Substrates
 Tumor Cell Lines

NEV10116

 Lentiviral Particles
 Optical Reporter Bacteria

In Vivo Imaging Instruments
Solutions and Support
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Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IVISense™ FLUORESCENT AGENTS

INVISense™ Fluorescent Agents
 Activatable Probes Targeted Probes

Fluorescent Dyes

 Vascular Probes

PerkinElmer’s IVISense fluorescent dyes for labeling antibodies, small molecules, proteins, or peptides are designed specifically to enable customized development of novel
superbright fluorescent imaging agents. Dye properties, such as excellent brightness, hydrolytic stability, and photostability, as well as selection for minimal interaction withbiological tissues with no potential loss of specificity, have been optimized and validated for use in in vivo or in vitro imaging.

 Cell Labeling Dyes

 Dyes Panels Agents by Disease

Available as NHS esters or maleimide reactive dyes for conjugation to either free amine (-NH3) or free thiol (-SH) containing molecules. Conjugate an optimal 1 to 3 dyes onto
your protein or choose self-quenching dyes to make your own custom fluorogenic probes.
645, 680, and 800 Fluorescent Dyes
FLUORESCENT DYEIVISense 645 NHS
Fluorescent Dye (1 mg)
IVISense 645 NHS
Fluorescent Dye (5 mg)
IVISense 645 MAL
Fluorescent Dye (1 mg)
IVISense 645 MAL
Fluorescent Dye (5 mg)
IVISense 680 NHS
Fluorescent Dye (1 mg)
IVISense 680 NHS
Fluorescent Dye (5 mg)
IVISense 680 MAL
Fluorescent Dye (1 mg)
IVISense 680 MAL
Fluorescent Dye (5 mg)
IVISense 800 NHS
Fluorescent Dye (1 mg)
IVISense 800 NHS
Fluorescent Dye (5 mg)
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PRODUCT DESCRIPTION
Red fluorescent amine-reactive dye for labeling
via an NHS ester to peptides, small molecules,
proteins, antibodies, or macromolecules.

Red fluorescent dye for coupling via maleimide
chemistry to label-free cysteines or thiolgroups on peptides, small molecules, proteins,antibodies, or macromolecules.

NIR fluorescent amine-reactive dye for labeling
via an NHS ester to peptides, small molecules,
proteins, antibodies, or macromolecules.

NIR fluorescent dye for coupling via maleimide
chemistry to label-free cysteines or thiolgroups on peptides, small molecules, proteins,antibodies, or macromolecules.

NIR fluorescent amine-reactive dye for labeling
via an NHS ester to peptides, small molecules,
proteins, antibodies, or macromolecules.

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IVISbrite™ Bioluminescent Reagents

680 Fluorescent Labeling Kit
CAT. NUMBER
NEV11173

FLUORESCENT DYEPRODUCT DESCRIPTIONIVISense 680 NHS
Fluorescent Labeling Kit

An easy and convenient way to label up to 10 mgof protein. The kit contains our superior in vivo
optimized dye and everything you need to carry out
the reaction and purify the labeled protein.

NEV11174
NEV11273

 Substrates
CAT. NUMBER

 Tumor Cell Lines

NEV11118

 Lentiviral Particles
 Optical Reporter Bacteria

In Vivo Imaging Instruments
680 and 750 Self-Quenching Fluorescent Dyes

NEV11274

FLUORESCENT DYEPRODUCT DESCRIPTION

CAT. NUMBER

IVISense 680 NHS FluorescentSelf-Quenching Dye (1 mg)

NEV11120

IVISense 680 NHS FluorescentSelf-Quenching Dye (5 mg)

Small-molecule NIR fluorescent dye to label atarget ligand. Optimized for single moleculeloading or to create your own custom
fluorogenic probe. Amine-reactive for labeling
via an NHS ester linkage.

NEV10121

NEV11119

IVISense 750 NHS FluorescentSelf-Quenching Dye (1 mg)

NEV11220

IVISense 750 NHS FluorescentSelf-Quenching Dye (5 mg)

Small-molecule NIR fluorescent dye to label atarget ligand. Optimized for single moleculeloading or to create your own custom
fluorogenic probe. Amine-reactive for labeling
via an NHS ester linkage.

NEV10123

NEV11219

NEV11107

IVISense 750 MAL FluorescentSelf-Quenching Dye (1 mg)

NEV11223

NEV11108

IVISense 750 MAL FluorescentSelf-Quenching Dye (5 mg)

Small-molecule NIR fluorescent dye to label atarget ligand. Optimized for single moleculeloading or to create your own custom
fluorogenic probe. Thiol-reactive for coupling
via maleimide chemistry to label-free cysteinesor thiol groups.

NEV10122

NEV10124

NEV11224

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Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IVISense™ FLUORESCENT AGENTS

INVISense™ Fluorescent Agents
 Activatable Probes Targeted Probes

Fluorescent Cell Labeling Dyes

 Vascular Probes

Label and track mammalian cells, including stem cells, T cells, macrophages, and more with IVISense fluorescent cell-labeling dyes. IVISense 680 NIR fluorescent lipophiliclabeling dyes intercalates into the cell membrane with an enhanced formulation that allows for quick solubilization in aqueous solution. With no impact on cell viability poststaining and excellent stability, it offers superior brightness and uniform labeling, ideal for cell detection in vitro and longitudinal cell tracking in vivo across many applications
including, inflammation, immunology, and stem-cell research.

 Cell Labeling Dyes

 Dyes Panels Agents by Disease

IVISense 750 DiR is a lipophilic, near-infrared (NIR) fluorescent cyanine dye for staining cytoplasmic membrane.

IVISbrite™ Bioluminescent Reagents
CELL LABELING DYEIVISense 680
Fluorescent Cell Labeling
Dye (1 x 0.2mg)
IVISense 680
Fluorescent Cell Labeling
Dye (5 x 0.2mg)
IVISense DiR 750
Fluorescent Cell
Labeling Dye

PRODUCT DESCRIPTION

 Substrates

CAT. NUMBER

 Tumor Cell Lines

NEV12001
NIR water-soluble cell-labeling agent that cangenerate brightly labeled and highly viable cells
suitable for detection and longitudinal tracking
in vivo. Each vial can stain up to 2 x 108 cells.

 Lentiviral Particles
 Optical Reporter Bacteria

NEV12000

In Vivo Imaging Instruments
NIR dye for non-invasive imaging of cell homing(stem cells, T cells).

Solutions and Support

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Macrophage tafficking to carrageenan-incuded inflamedpaw (right) and control paw (left) using IVISense 680
fluorescent cell-labeling dye. Imaged using the IVIS
Spectrum system.

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Thioglycollate-elicited
peritoneal macrophageslabeled with IVISense 680
fluorescent cell labeling dye(bottom) or unlabeled (top).

Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IVISense™ FLUORESCENT AGENTS

INVISense™ Fluorescent Agents
 Activatable Probes Targeted Probes

Fluorescent Imaging Panels

 Vascular Probes

With PerkinElmer’s fluorescent in vivo imaging panels, choosing the probes you need for your research has never been easier. Specially curated using our most publishedagents, IVISense Fluorescent Panels contain probes that are carefully bundled together to target key disease areas.

 Cell Labeling Dyes

Designed to optimize your findings and maximize your in vivo imaging research studies, IVISense Fluorescent Panels enable the early detection and characterization ofmultiple disease-specific biomarkers in the same subject, for longitudinal bioprofiling over the course of the study.

 Dyes Panels Agents by Disease

IVISbrite™ Bioluminescent Reagents
FLUORESCENT PANELPART NOUSED TO

Arthritis Panel

NEV20003

Detect molecular players in arthritis pathology.

IVISense FLUORESCENT PANEL CONTENTS
Vascular 750
CAT B 750 FAST

CAT K 680 FAST
MMP 680

Osteo 680
Pan Cathepsin 680

Folate Receptor 680
MMP 750 FAST

Pan Cathepsin 680
Transferrin-Receptor 750

Tumor Metabolism Panel

NEV20015

Non-invasively measure tumor microenvironment biology for early detectionof treatment-induced biological changes.

Vascular 680
Annexin-V 750
Bombesin Receptor 680

Toxicology Panel

NEV20016

Detect molecular players and measure early changes in drug-induced injury.

Vascular 680
Annexin-V 750

MMP 750 FAST*
Renin Receptor 680 FAST

Transferrin-Receptor 750

Oncology Panel

NEV20005

Non-invasively characterize the tumor environment to assess biologicalchanges associated with disease progression or treatment efficacy.

Vascular 680
Folate Receptor 680

Integrin Receptor 750
MMP 750 FAST

Pan Cathepsin 750

Inflammation Panel

NEV20013

Quantify inflammation biology, particularly in longitudinal studies forunderstanding disease pathogenesis.

Vascular 680
Folate Receptor 680

Integrin Receptor 750
MMP 750 FAST

Neutrophil Elastase
680 FAST
Pan Cathepsin 750 FAST

Vascular Panel

NEV20014

Characterize biological changes associated with vascular injury and disease.

Vascular 680
CAT B 680 FAST

Integrin Receptor 750
MMP 750 FAST

Pan Cathepsin 750 FAST

Featured Probes Pack

NEV20011

Approach different research areas by sampling our most popular andpublished fluorescent probes.

Vascular 680
Folate Receptor 680

Integrin Receptor 750
MMP 750 FAST

Pan Cathepsin 680
680 NHS FluorescentLabeling Kit

Sample Pack

NEV20000

Combine fluorescence and bioluminescent imaging by sampling our mostpopular and published fluorescent probes, as well as ready-to-use injectableluciferin for bioluminescent imaging.

Vascular 680
Integrin Receptor 750

MMP 750 FAST
Pan Cathepsin 750

IVISbrite™ D-Luciferin RediJect™ Solution*2 MMP are provided for the Annexin/MMP/Transferrin cocktail as detailed in the Fluorescent Panel User Guide.
All panels contain full size fluorescent probes (10 doses) except for the Sample Pack which contains sample sizes (3 doses) of each fluorescent probe and 1 full size IVISbrite D-Luciferin - RediJect Solution (50 doses).
Panels are not open to substitution.

9|

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 Substrates
 Tumor Cell Lines Lentiviral Particles
 Optical Reporter Bacteria

In Vivo Imaging Instruments
Solutions and Support
Contact Us

Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTSINVISense™ Fluorescent Agents
Vascular
Panel

Inflammation
Panel

Oncology
Panel

Toxicology
Panel

Tumor
Metabolism
Panel

Arthritis
Panel

Vascular
DiseasePulmonary
Disease

Oncology

KidneyFunction

Inflammation

Infectious
DiseaseHypertension

Cardiovascular
DiseaseBone Loss

Atherosclerosis

Arthritis

Angiogenesis

IVISense™
FLUORESCENT
AGENTS

Apoptosis

Introduction

IVISense Cat B 680 FAST

Built around yourapplications – choose
one, or use in combination
for your disease focus toobtain more information.

IVISense MMP 645 FAST

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IVISense Cat B 750 FAST
IVISense Cat K 680 FAST
IVISense MMP 680
IVISense MMP 750 FAST
IVISense Neutrophil Elastase 680 FAST
IVISense Pan Cathepsin 680
IVISense Pan Cathepsin 750
IVISense Pan Cathepsin 750 FAST

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IVISense Renin Receptor 680 FAST

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 Panels Agents by Disease

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IVISbrite™ Bioluminescent Reagents Substrates

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IVISense Integrin Receptor 750

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IVISense Hypoxia CA IX 680

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IVISense IVISbrite MPO 425 - RediJect Solution
IVISense Osteo 680

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IVISense Osteo 750
IVISense Osteo 800
IVISense Tomato Lectin 680

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IVISense Folate Receptor 680
IVISense Integrin Receptor 645

 Optical Reporter Bacteria

IVISense Bacterial 750 - RediJect Solution

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In Vivo Imaging Instruments
Solutions and Support
Contact UsIVISense Bombesin Receptor 680
IVISense Transferrin-Receptor 750

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Vascular and physiologic fluorescence agents are distributed passively through blood vessels to enable imaging of vascularity, blood pooling near tumors, and edema
IVISense Vascular 680
IVISense Vascular 750
IVISense Vascular NP 680
IVISense Vascular NP 750
IVISense Edema 680

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 Tumor Cell Lines Lentiviral Particles

Targeted probes enable specific areas of interest to be detected, monitored, and measured in vivo
IVISense Annexin-V 750

 Dyes Cell Labeling Dyes

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IVISense 2-DG 750 RediJect Solution

 Targeted Probes Vascular Probes

Easily activated fluorescence probes enable specific imaging of biological processes that underlie disease

Fluorescent
Agents by
Disease Area

 Activatable Probes

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Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IVISbrite™ BIOLUMINESCENT REAGENTS

INVISense™ Fluorescent AgentsThe Sensitivity and Reproducibility
You Need
Get the sensitivity and reproducibility you need from your in vivo imaging
studies with our in vivo validated substrates, lentiviral particles, luciferase
tumor cell lines, and bacteria.

Bioluminescent/Chemiluminescent Substrates
PerkinElmer’s IVISbrite bioluminescent substrates are available in easy-touse formats that fit your in vivo imaging laboratory workflow. Choose fireflyD-luciferin for use in your in vitro or in vivo applications, such as with our Red
F-luc (Luciola italica) luciferase, or coelenterazine h, the substrate of manyaquatic luciferases such as our Renilla reniformis luciferase.
RediJect™ solution – a pre-formulated, ready-to-use format that reduces
preparation time and effort while still delivering ultimate sensitivity andreproducibility that is critical for accurate quantitation.
Lyophilized D-Luciferin – offers the same sensitivity and high performanceas the RediJect formulation. Available in 1 gram and higher quantities forvolume savings.

PRODUCT DESCRIPTIONIVISbrite D-Luciferin
Potassium Salt (1g)

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 Vascular Probes
 Dyes Cell Labeling Dyes

IVISbrite D-Luciferin
Potassium Salt (5 x 1g)

CAT. NUMBER

Lyophilized bioluminescence substrate for in vivo
imaging with firefly luciferase

IVISbrite D-Luciferin
Potassium Salt (10 x 1g)

122799-5

IVISbrite™ Bioluminescent Reagents

122799-10

 Tumor Cell Lines

 Substrates
 Lentiviral Particles

IVISbrite D-Luciferin in
RediJect Solution

Pre-formulated in PBS, batch-controlled D-luciferin(K+ salt) ready for in vivo use

770504

IVISbrite D-Luciferin Ultra
in RediJect Solution

Pre-formulated in PBS, batch-controlled D-luciferin(K+ salt) for in vivo use. Includes a rapidly clearing
fluorescent marker to validate substrate injection

770505

IVISbrite Coelenterazine h
in RediJect Solution

Pre-formulated in PBS, batch controlled
coelenterazine h for in vivo use

760506

IVISbrite MPO 425
Chemiluminescent Probe in
RediJect Solution (1 vial) Agents by Disease122799760535Pre-formulated in PBS, chemiluminescent probe formonitoring inflammation

Kinetics of IVISbrite
D-luciferin RediJect
bioluminscence imaging
in a flank 4T1 mousebreast adenocarcinoma.

| Targeted Probes Panels
PRODUCT

IVISbrite MPO 425
Chemiluminescent Probe in
RediJect Solution (4 vials)

11 Activatable Probes

760536

 Optical Reporter Bacteria

In Vivo Imaging Instruments
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Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IVISbrite™ BIOLUMINESCENT REAGENTS

INVISense™ Fluorescent Agents
 Activatable Probes Targeted Probes

Brighter, Red-Shifted Tumor Cell Lines Vascular Probes

Expand into orthotopic deep tissue oncology models with brighter, red-shifted celllines. PerkinElmer’s IVISbrite™ bioluminescent light-producing tumor cell lines are
significantly brighter than other firefly luciferases. These cells have been stablytransduced with the red-shifted firefly luciferase gene from Luciola Italica (RedF-luc), for a brighter signal. By emitting intensified, longer wavelength light, ourbioluminescent tumor cell lines allow you to visualize and monitor the growth of deep
tissue tumors in vivo. The optimized Red F-luc luciferase enables more sensitive
imaging with less tissue attenuation so you can detect tumor development earlier andmonitor tumor growth and metastases in both subcutaneous and orthotopic models.

IVISbrite Tumor Cell Lines Labeled With Enhanced Red F-Luc Vector

 Dyes Cell Labeling Dyes
 Panels Agents by Disease

Bioluminescence imaging
of IVISbrite HCT-116 Red
F-luc subcutaneous tumor.
Imaged using the IVIS
Spectrum.

Bioluminescenceimaging of IVISbrite U87
MG Red F-luc orthotopic
tumor. Imaged using the
IVIS Spectrum.

In Vivo Comparison: Five million of both IVISbrite HepG2 Red
F-luc tumor cells and competitor luciferase transduced HepG2
cells were injected s.c. in the flank of nude mice; tumors wereimaged after five weeks. Red F-luc transduced cells generate 15
times brighter bioluminescence signal than the corresponding
tranduced cells despite similar tumor size. (Peterson, et al. 2014).
Brightness varies by cell line.

IVISbrite™ Bioluminescent Reagents
 Substrates
 Tumor Cell Lines Lentiviral Particles
 Optical Reporter Bacteria

IVISbrite Tumor Cell Lines Labeled With Enhanced Red F-Luc Vector

TUMOR CELL LINEPRODUCT DESCRIPTION

CAT. NUMBER

TUMOR CELL LINEPRODUCT DESCRIPTION

CAT. NUMBER

IVISbrite U-87 MG Red F-luc

Brain, glioblastoma (Human)

BW 124577

IVISbrite NCI-H460 Red F-luc

Lung adenocarcinoma (Human)

BW 124316

IVISbrite GL261 Red F-luc

Brain, glioma (Murine)

BW 134246

IVISbrite SKOV3 Red F-luc

Ovarian adenocarcinoma (Human)

BW 119276

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IVISbrite 4T1 Red F-luc

Breast mammary adenocarcinoma (Murine)

BW 124087

IVISbrite BxPC3 Red F-luc

Pancreatic adenocarcinoma (Human)

BW 125058

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IVISbrite MCF-7 Red F-luc

Breast mammary adenocarcinoma (Human)

BW 119262

IVISbrite PC-3 Red F-luc

Prostate adenocarcinoma (Human)

BW 128444

IVISbrite HCT-116 Red F-luc

Colorectal carcinoma (Human)

BW 124318

IVISbrite LnCaP Red F-luc

Prostate, supraclavicular lymph node carcinoma (Human)

BW 125055

IVISbrite HT-29 Red F-luc

Colorectal carcinoma (Human)

BW 124353

IVISbrite B16-F10 Red F-luc

Skin melanoma (Murine)

BW 124734

IVISbrite Colo205 Red F-luc

Colorectal adenocarcinoma (Human)

BW 124317

IVISbrite HT1080 Red F-luc

Fibrosarcoma (Human)

BW 128092

IVISbrite HepG2 Red F-luc

Hepatic carcinoma (Human)

BW 134280

IVISbrite K-562 Red F-luc

Leukemia, Chronic Myelogenous Leukemia (Human)

BW 124735

IVISbrite A549 Red F-luc

Lung carcinoma (Human)

BW 119266

IVISbrite LL/2 Red F-luc

Lung carcinoma (Murine)

BW 119267

12|

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IVISbrite Tumor Cell Lines Dual Labeled With Enhanced Red F-Luc Vector and Green
Fluorescent Protein (GFP)
TUMOR CELL LINEPRODUCT DESCRIPTION

CAT. NUMBER

IVISbrite 4T1-Red-Fluc-GFPBreast mammary adenocarcinoma (Murine) duallabeled with luciferase and GFP

BW 128090

IVISbrite PC-3-Red-Fluc-GFP

Prostate adenocarcinoma (Human) dual-labeled withluciferase and GFP

BW 133416

In Vivo Imaging Instruments

Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IVISbrite™ BIOLUMINESCENT REAGENTS

INVISense™ Fluorescent Agents
 Activatable Probes Targeted Probes

Lentiviral Particles – Create the Bioluminescent Cell Line You Need Vascular Probes

Stably transduce cells with lentivirus particles expressing a combination of novel luciferases and a fluorescent protein for monitoring and tracking cells in vivo.

 Cell Labeling Dyes

IVISbrite lentiviral particles are self-inactivating vectors containing the brighter, red-shifted Red F-luc firefly luciferase (Luciola italica) or green-shifted Renilla luciferase (Renilla
reniformis) transgene under control of the stable UbC promoter. These lentiviral particles enable efficient transduction of a wide variety of mammalian cells including mostcancer cell lines, primary, stem, and non-dividing cells. Use our bioluminescent D-luciferin or coelenterazine substrates to image your transduced cells in vivo.

 Dyes Panels Agents by Disease

IVISbrite™ Bioluminescent Reagents

PRODUCTPRODUCT DESCRIPTION

CAT. NUMBER

IVISbrite Red F-luc-Puromycin Lentiviral Particles

Red-shifted firefly luciferase with puromycin as selection marker

CLS960002

IVISbrite Red F-luc-GFP Lentiviral Particles

Red-shifted firefly luciferase and green fluorescent protein (GFP)

CLS960003

 Lentiviral Particles

IVISbrite Green Renilla-Puromycin Lentiviral Particles

Green Renilla luciferase with puromycin as selection marker

CLS960004

 Optical Reporter Bacteria

 Substrates
 Tumor Cell Lines

In Vivo Imaging Instruments
Solutions and Support
Contact Us

In vivo bioluminescence imaging ofmice implanted with PC3 prostatecancer cells transduced by IVISbrite
Red F-luc Puromycin Lentiviral
Particles (week 1, 2, 3, 4, and 5).

13|

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Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IVISbrite™ BIOLUMINESCENT REAGENTS

INVISense™ Fluorescent Agents
 Activatable Probes Targeted Probes

Optical Reporter Bacteria

 Vascular Probes

Optical in vivo imaging technology has been successfully used to non-invasively measure the spread of infection, monitor infection dynamics, and determine the in vivo efficacy
of antimicrobial compounds in various infectious disease models. PerkinElmer offers a range of bacterial strains labeled with the Photorhabdus luminescens lux operon (including
biofilm forming strains) for a variety of in vitro and in vivo applications. One advantage of bacterial luciferase is that it negates the use of an exogenous substrate such as luciferin.

 Cell Labeling Dyes
 Panels Agents by Disease

BACTERIUM

PARENTAL STRAIN

IVISbrite Escherichia coli Xen14

EPEC WS2572

119223

IVISbrite Listeria monocytogenes Xen19

ATCC 23074

119237

 Tumor Cell Lines

IVISbrite Pseudomonas aeruginosa Xen5

ATCC 19660

119228

 Lentiviral Particles

IVISbrite Pseudomonas aeruginosa Xen41

PAO1

119229IVISbrite Shigella dysenteriae Xen27

88A6205 Clinical isolate

119231

IVISbrite Proteus mirabilis Xen44

ATCC 51286

119236

IVISbrite Salmonella typhimurium Xen33

FDA1189

119235

IVISbrite Yersinia enterocolitica Xen24

91A1854 Clinical isolate

119232

IVISbrite Yersinia enterocolitica Xen25

WS2589119233

IVISbrite Staphylococcus aureus Xen8.1

8325-4119239IVISbrite Staphylococcus aureus Xen29

ATCC 12600

119240

IVISbrite Staphylococcus aureus Xen31

ATCC 33591

119242

IVISbrite Staphylococcus aureus Xen36

ATCC 49525

119243

IVISbrite Staphylococcus aureus Xen40

UAMS-111924414|

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CAT. NUMBER

 DyesIVISbrite™ Bioluminescent Reagents
 Substrates

 Optical Reporter Bacteria

In Vivo Imaging Instruments
IVISbrite Pseudomonas aeruinosa
Xen5 imaged on IVIS Spectrum.

Solutions and Support
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Real-time non-invasive
imaging of IVISbrite
Proteus mirabilis Xen44
migration of UTI infection
from the bladder to thekidney. Imaged using IVIS
Spectrum system.

IVISbrite Pseudomonas
aeruginosa Xen5 biofilm
infection. Imaged using
the IVIS Spectrum.

Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction

IN VIVO IMAGING SYSTEMSINVISense™ Fluorescent Agents
 Activatable Probes Targeted Probes Vascular Probes

PerkinElmer offers a wide range of imaging systems designed to fit your research and development needs.

 Dyes Cell Labeling Dyes
 Panels Agents by Disease

IVISbrite™ Bioluminescent Reagents
 Substrates
 Tumor Cell Lines Lentiviral Particles
 Optical Reporter Bacteria

In Vivo Imaging Instruments

IVIS Spectrum
Optical Imaging Platform

IVIS Lumina S5 and X5
Optical Imaging Systems

IVIS Lumina Series III
Optical Imaging Systems

Quantum GX2
microCTn 2D bioluminescence andfluorescence imaging

n High-sensitivity 2D bioluminescenceand fluorescence imaging

n 2D bioluminescence andfluorescence imaging

n High-resolution microCT imaging offine anatomical structures

n 3D optical tomographyn High-throughput format withsimultaneous 5-mouse imaging

n Low-dose X-ray with optical overlay(IVIS Lumina XRMS only)n High-speed scanningn High-resolution X-ray with optical
overlay (IVIS Lumina X5 only)n Images up to three mice
simultaneously

n Compact design that fits on thebenchtopn Compact design that fits on
your benchtop

n Integrated CT adding anatomical
context to your optical studies(IVIS SpectrumCT only)n Simultaneous imaging of five micewith ability to expand to 10 mice
(optional upgrade)15|

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n Streamlined imaging workflow withanimal management accessories

n Low-dose imaging ideal for
longitudinal in vivo studies
n Images ex vivo samples, as well as
mice, rats, and rabbits in vivon Seamless co-registration with
IVIS systems

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Bioluminescent and Fluorescent In Vivo Imaging Agents

TABLE OF CONTENTS
Introduction
INVISense™ Fluorescent AgentsThe support you need to achieve
your research goals

 Activatable Probes Targeted Probes Vascular Probes
 Dyes Cell Labeling Dyes
 Panels Agents by Disease

IVISbrite™ Bioluminescent Reagents
 Substrates
 Tumor Cell Lines Lentiviral Particles
 Optical Reporter Bacteria

In Vivo Imaging Instruments
Our experienced team of scientists
and support specialists are here tohelp you. From application questions
and training to troubleshooting
advice and helping you select thebest imaging system and reagentsfor your research, we have theresources you need to get the mostout of your in vivo imaging studies.

16|

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Application Support

Training and Education

Service

Offering worldwide support, ourdedicated applications team with
their in-depth scientific knowledge
can help you achieve your researchgoals. By offering unmatchedsupport, our application scientists are
your main contact for any questions
you may have from developmentof imaging protocols to training
and education ensuring you get theanswers and guidance you need.

Get the most from your in vivo
imaging research by learning fromthe experts. We offer basic andadvanced training at your facility as
well as classroom training throughIn Vivo University to broaden yourknowledge, share with fellow
researchers, and learn from field
application scientists.

Our global service engineers are available
to help you with installation, preventative
maintenance, technical guidance, andany repair services to ensure that yourimaging system operates as optimallyas possible to meet your in vivo imaging
research goals.

Solutions and Support
Contact Us

For more information visit www.perkinelmer.com/category/in-vivo-imaging-reagents

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