GC Technical Note

GT092

Purity Test for Xylene

GL Sciences Inc.

There are three isomers of xylene: o-xylene, m-xylene, and p-xylene. The JIS standard includes thetotal content of o-, m-, and p-xylene as 80% or more, and benzene at 0.1% or less, Toluene is definedas 1.0% or less. The purity test of xylene was performed with reference to the calculation method
for JIS relative sensitivity.

Measurement procedure
Measure the sample for relative sensitivity anddetermine the relative sensitivity

Preparation of samples for relative
sensitivity determinationRelative sensitivity of compound i
Mass (g) of Ethylbenzene
Peak area of Ethylbenzene
Peak area of compound i
Mass (g) of compound i
Xylene was measured.
Determine the content of each component

Amount of compound i contained
Peak area of compound i
Relative sensitivity of compound i
Number of all the peaks

Weigh o-Xylene, m-Xylene, p-Xylene, Ethylbenzene,Benzene and Toluene to 1g on a balance and mix.
Samples were weighed to an accuracy of 1 mg.
Weight valueo-Xylene

1.0103 gm-Xylene

0.9999 gp-Xylene

1.0131 g

Ethylbenzene1.0035 g

Benzene1.0046 g

Toluene1.0026 g

Determine the purity of xylenes

Assay conditions
Conditions
SystemColumn
Col. Cat. No.
Col. Temp.
Carrier Gas
Injection
Detection
Injection Vol.
Syringe Size

: GC - FID: InertCap 1 0.53 mm I.D. x 30 m df = 1.00 μm: 1010-11445: 80 ˚C (15 min) - 10 ˚C/min - 180 ˚C (5 min)
: He 3.00 mL/min: Split 10:1200 ˚C: FID Auto Range
200 ˚C: 0.2 μL
: 10 μLGL Sciences GC Technical Note

Measurement
Chromatogram of the sample for relative sensitivity determination

20000

Analyte
1. Benzene
2. Toluene
3. Ethylbenzene
4. m-Xylene
5. p-Xylene
6. o-Xylene

12mVolt
10000

4+53060246Time (min)81012mVolt
20Analyte

4+524020000

Chromatogram of xylene

1. Benzene
2. Toluene
3. Ethylbenzene
4. m-Xylene
5. p-Xylene
6. o-Xylene

mVolt
10000

0124Time (min)66320

10246Time (min)81012GL Sciences GC Technical Note
Relative standard deviation
The relative standard deviation was determined for the measurement accuracy of a 0.2 μL
injection volume with a 10 μL syringe.
Table 1. Repeatability of area values (xylene, n = 7)
1 st
2 nd
3 rd
4 th
5 th
6 th
7 th
Ave.
SDRSD (%)

Benzene
13381
13098
13068
13092
13350
13142
13185
13188
127
0.97

Toluene Ethylbenzene m- Xylene + p- Xylene
205266413
108558 17274370
201390140
106438 16946836
201112183
105879 16896183
200496966
106011 16854562
205228799
108631 17274375
202445574
106774 17031437
107261 17098290
203319807
202751412
107079 17053722
1936575
1134
171155
0.96
1.06
1.00

o- Xylene
3258612531968917
31888650
31796033
32595549
32150952
32286799
32181861
323191
1.00

Relative sensitivity to ethylbenzene
Table 2. Relative sensitivity of each component
Weight (g)相対感度用試料の面積値
相対感度Benzene1.004
43382675
0.94

TolueneEthylbenzene m- Xylene + p- Xylene o- Xylene
1.002
1.003
1.013
0.999
1.01
45604480
45938507
9211113146622364
0.99
1.00
1.00
1.01

Purity of xylenes
Table 3. The product contains components as shown belowBenzene
含有量(%)

Toluene0.00550.043

Ethylbenzene
6.8m -Xylene + p -Xyleneo -Xylene

8113The purity of xylenes (sum of the content of o-, m-, and p-xylenes) was 94 %,Benzene content was 0.0055 % and toluene 0.043 %.

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly.
We reserve the right to amend this information or data at any time and without any prior announcement.

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