GC Technical Note

GC/MS Metabolites Library

GT081

GL Sciences Inc.

There are several methods with GC to analyze metabolites, such as amino acids, organic acids,
fatty acids, sugars, and so on. Methoximation and trimethylsilylation (TMS) is the classical and mostwidely used derivatization procedure for metebolome analysis with GC.
In this note, the about 400 metabolites widely used TMS derivatized were detected with GC-MS(the column was InertCap 5MS Metabolomics).

Derivatizing procedure
Evaporated sample (～about 10 mg)＜Methoximation＞
100 μL of methoxyamine hydrochloride in pyridine (20 mg/mL) Incubation(30 ºC, 90 min)＜Trimethylsilylation＞ 50 μL of MSTFA (Cat.No. 1022-11061)
Incubation (37 ºC, 30 min)Derivatized sample

GC/MS Conditions
SystemColumnInjection Inj. Temp.
Oven Temp.
Carrier gas
Carrier gas control
Septum purge flow
Split ratio Interface
Temp.
Ion source Temp.
Ionization Vortage
Scan range
Injection Vol.

: GC-MS: InertCap 5MS Metabolomics
df = 0.25 µm
0.25 mm I.D. × 30 m
(Cat.No. 1010-18952): Split: 230 ºC
: 80 ºC (2 min) - 15 ºC /min - 330 ºC (13 min): Helium: Constant Pressure 75 kPa: 5 mL/min: 1:25: 250 ºC
: 200 ºC
: 70 eV: m/z 85 - 500: 1 μLGL Sciences GC Technical Note

Compounds
C10
Propyleneglycol
n-Propylamine
2-Hydroxypyridine
PyruvateOxalacetic acid
α-Methylbenzylamine_1
Lactic acid
Glycolic acidn-Methylethanolamine
Valine_1
C11
Isobutylamine
Alanine_1
n-Butylamine
Ketovaline_1
Glycine_1
2-Hydroxybutyrate
OxalateKetovaline_2
Sarcosine
2-Aminoisobutyrate
4-Hydroxypyridine
3-Hydroxybutyrate
2-Aminobutyric acid
Ketoisoleucine_1
C12
Norleucine_1
Ketoisoleucine_2
Malonic acid
Glyceraldehyde_1
1-Aminocycropropane-1-carboxylic acid
α-Methylbenzylamine_2
Valine_2
Glyceraldehyde_2
Urea
Norvaline
Benzoic acid
Dihydroxyacetone
Oxamic acid
Serine_1
n-Caprylic acidn-Acetyl-DL-alanine
2-Aminoethanol
LeucinePhosphate
GlycerolEthylmalonate
2-Oxobutyrate_1
Nicotinic acid
3-Hydroxypyruvate_1
C13
Threonine_1
Isoleucine
ProlineMaleic acid
2-Oxobutyrate_2
Succinic acid
Glycine_2

Retention
Indices
1000
1002
1026
1038
1049
1049
1051
1064
1077
1082
1091
1100
1100
1106
1111
1114
1124
1132
1134
1140
1143
1147
1154
1165
1177
1185
1200
1204
1206
1208
1213
1214
1224
1225
1227
1236
1248
1251
1260
1260
1264
1264
1264
1275
1280
1282
1283
1285
1289
1294
1295
1300
1302
1303
1307
1309
1315
1317
1318

RetentionTime(s)257.88258.99276.44284.99292.71292.86294.78303.72313.46317.02322.90329.64329.76333.59337.08339.23346.02351.69353.38357.33359.28362.05367.10374.70383.33389.00399.18401.51403.08404.25407.84407.99414.72415.49416.96422.71430.04432.56437.88438.09440.66440.72440.95447.60451.26452.70453.00454.41457.05460.31460.79464.10465.17466.07468.20469.43472.99474.08474.76

Cas.No.
57-55-6
107-10-8
142-08-5
127-17-3
328-42-7
618-36-0
79-33-4
79-14-1
109-83-1
72-18-4
78-81-956-41-7
109-73-93715-29-5
56-40-65094-24-6
144-62-7
107-97-1
62-57-7
626-64-2
300-85-6
1492-24-6
3715-31-9
327-57-1
141-82-2
56-82-622059-21-8

57-13-6
760-78-1
65-85-0
96-26-4
471-47-6
56-45-1
124-07-2
1115-69-1
141-43-5
61-90-57664-38-2
56-81-5
601-75-2
600-18-0
59-67-61113-60-6
72-19-5
73-32-5
147-85-3
141-82-2
110-15-6

GL Sciences GC Technical Note

Compounds
2-Phenylpropionate
2-Picolinate
CatecholOxamideNorleucine_2
2-Methyl benzoic acid
Methylsuccinic acid
Glyceric acid
3-Hydroxypyruvate_2
UracilFumaric acid
Nonanoric acid
4-Methyl benzoic acid
Serine_2
Alanine_2
Pipecolic acidn-Acetyl-DL-valine
β-Cyano-L-alanine
n-Formylglycine
3-Pyridylacetic acid
Ketoleucine
Threonine_2
C14
Allothreonine
Glutaric acid
Thymine2-Methylglutarate
2,3-Bisphospho-glycerate
Hydrocinnamate
3-Methylglutarate
Phenoxyacetic acid
Cinnamyl alcohol
β-Alanine
n-Acetyl-L-leucine
Maleamic acid
Homoserine
Nicotinamide
Citramalic acid
Madelic acid
Malic acid
p-Hydroxybenzaldehyde
C15
Homoserine lactone
Adipic acid
4-Hydroxybenzylalcohol
Threitol
Acetylsalicylic acid
Meso-erythritol
Methionine
2-Thiouracil
Aspartic acidα-Phenylglycine
Cytosine
Pyroglutamic acidtrans-4-Hydroxy-L-proline
4-Aminobutyric acid
Pyrogallol
5-Methylcytosine
3-Hydroxybenzoate

Retention
Retention
IndicesTime(s)1322
477.401324
478.251326
479.771326
480.001328
480.881329
481.851330
482.241341
488.721342
489.321345
491.461349
493.331361
500.701368
504.811371
506.891373
508.11
1374
508.531380
512.391381
512.641382
513.651392
519.191393
519.841399
523.501400
524.281404
526.681406
527.641409
529.561419
535.171420
535.451425
538.101428
540.181430
540.851433
542.771438
545.861444
549.151445
549.311461
558.551484
571.661486
572.841488
573.61
1498
579.191499
580.091500
580.441504
582.501509
585.151512
586.591516
588.701522
592.291525
593.471531
596.791531
596.821531
596.871532
597.541533
597.941535
598.731540
601.471543
602.931561
612.561563
613.731573
619.07

Cas.No.
492-37-5
98-98-6
120-80-9
471-46-5
118-90-1
498-21-5
473-81-4
66-22-8
110-17-8
112-05-0
99-94-5535-75-1
3067-19-4
6232-19-5
2491-15-8
6419-36-9
816-66-0
144-98-9
110-94-1
65-71-4
18069-17-5
102814-02-8
501-52-0
626-51-7
122-59-8
104-54-1
107-95-9
1188-21-2
557-24-4
672-15-1
98-92-06236-10-8
611-71-2
6915-15-7
123-08-0
6305-38-0
124-04-9
623-05-2
2418-52-2
50-78-2
149-32-6
63-68-3
141-90-2
56-84-8
875-74-1
71-30-7
149-87-1
51-35-4
1956-12-2
87-66-1
58366-64-6
1999-6-9

GL Sciences GC Technical Note

Compounds
Creatinine
Threonic acid
Acetoacetic acid
6-Hydroxynicotinic acid
2-Hydroxyphenylacetic acid
1-Hydroxyphenethyl alcohol
1-Methyluracil
α-Ketoglutaric acid
3-Amino-2,3-dihydrobenzoic acid
Ethionine2-Isopropylmalic acid
Prephenate
3-Phenyllactic acid
C16
Heptanedioate
Phosphoenolpyruvic acid
3-Hydroxy-3-methylglutarate
3-Hydroxyphenylacetic acid
Hypotaurine
β-Glutamic acid
Anthranilic acid
Glutamic acid
4-Hydroxybenzoic acid
5-Aminovaleric acidPhenylalanine
Paeonol_1
4-Hydroxyphenylacetic acid
Lauric acid
Tartrate
Paeonol_2
1,3-Propanediamine
Prolinamide
Xylose_1
Lyxose_1
threo-3-Hydroxy-L-aspartic acid
Homocysteine
Xylose_2
5-Hydroxymethyluracil
Lyxose_2
Asparagine
Arabionose
Kojic acid
TaurineN-Formyl methionine_1
Cysteine sulfinic acid
Methyl jasmonate_1
BicineXylulose
C17
Ribulose
Suberic acid
Ribosen-Formyl methionine_2
Phthalic acid
1,6-Anhydroglucose
2-Aminoadipic acid
Xylitol
Methyl jasmonate_2
Quinolinic acid

Retention
Retention
IndicesTime(s)1576
620.271577
620.991579
622.021579
622.091580
622.62
1580
622.71
1582
623.81
1582
623.841589
627.131594
630.001595
630.321597
631.52
1599
632.601600
633.121606
635.951612
639.01
1614
639.941618
641.831618
641.901623
644.661625
645.401630
647.971636
650.881641
653.41
1643
654.481644
654.781648
657.001653
659.371657
661.201664
664.971669
667.141669
667.191673
669.081673
669.501676
670.791678
671.641679
672.271680
672.751683
674.491684
674.701685
675.451686
675.541686
675.951689
677.441695
680.031698
681.481699
682.05
1700
682.63
1700
682.73
1700
682.82
1700
682.901701
683.131705
684.81
1706
685.691726
694.881727
695.321734
698.701738
700.631742
702.54

Cas.No.
60-27-570753-61-6
3483-11-2
5006-66-6
614-75-5
501-94-0
615-77-0
328-50-759556-17-1
67-21-03237-44-3
2931-08-0
103-82-2
111-16-0
138-08-9
503-49-1
621-37-4
300-84-5
1948-48-7
118-92-3
56-86-0
99-96-7
660-88-8
63-91-2
552-41-0
156-38-7
143-07-7
87-69-4
109-76-2
7531-52-4
58-86-6
1949-78-6
4294-45-5
454-29-5
4433-40-3
70-47-3
147-81-9
501-30-4
107-35-7
4289-98-9
207121-48-0
39924-52-2
150-25-4
551-84-8
488-84-6
505-48-6
50-69-1
88-99-3
498-07-7
1118-90-7
88-99-0
89-00-9

GL Sciences GC Technical Note

Compounds
Glycerol-2-phosphate
Rhamnose_1
Arabitol
RibitolAconitate
Rhamnose_2
Orotic acid
Fucose_1
Putrescine
2,3-Dihydroxybenzoate
Ciliatine
1,3-Benzenedicarboxylic acid
2,6-Pyridinedicarboxylic acid
Fucose_2n-Acetyl-L-Glutamate_1
1-Methylhistamine
5-Amino-levulinate_1
Theanine_1
3-Methoxy-4-hydroxyphenylacetate
Glutamine
Gentisic acidn-Acetyl-L-glutamate_2
Dihydroorotate
Theanine_2
4-Hydroxymandelate
Xylonic acidC18
1,4-Benzenedicarboxylic acid
O-Phosphoethanolamine
5-Amino-levulinate_2
Indole-3-acetaldehyde_1
2-Deoxy-D-glucose
Phenanthrene
Hippurate_1
Hypoxanthine
Shikimic acid
2-Aminopimelic acid
1-Methyl histidine_1
Anthracene
Dimethylbenzimidazole
3,4-Dihydrophenylacetic acid
3,4-Dihydroxybenzoate
Glycyl-glycine_1
Citrulline
Ornithine
Isocitric acid
Glycyl-glycine_2
Citric acid
Methionine sulfone
Hippurate_2
Homogentisate
2-Methylhippurate_1
Alanyl-alanine_1
o-Phospho-L-serine
Cadaverine
Caffeine
Lysine_2
Histidinol
5-Hydroxyanthranilic acid

Retention
Indices
1745
1746
1746
1752
1754
1755
1756
1756
1758
1760
1764
1766
1768
1770
1771
1776
1778
1778
1783
1786
1789
1790
1792
1792
1792
1794
1800
1802
1804
1805
1809
1816
1818
1819
1820
1820
1822
1825
1829
1829
1834
1834
1835
1836
1838
1838
1838
1840
1846
1849
1849
1851
1852
1857
1858
1867
1868
1870
1871

RetentionTime(s)703.87704.15704.48707.15707.91708.47709.17709.19709.97711.08712.57713.57714.47715.42716.19718.23719.28719.54721.89723.18724.56724.87725.83726.03726.11726.70729.66730.70731.60731.70733.67736.83737.79738.03738.53738.71739.40740.88742.66742.72744.74744.76745.13745.93746.54746.72746.81
747.34750.13751.45751.58752.47752.90755.35755.70759.79760.10760.97761.50

Cas.No.
819-93-010485-94-6
488-82-4
488-81-3
585-84-2
65-86-1
2438-80-4
333-93-7
303-38-8
2041-14-7
121-91-5
499-83-2
1188-37-0
6481-48-7
5451-9-2
3081-61-6
306-08-1
5959-95-5
490-79-9
5988-19-2
184901-84-6
526-91-0
100-21-0
1071-23-4
20095-27-6
154-17-6
1985-1-8
495-69-2
68-94-0
138-59-0
627-76-9
332-80-9
120-12-7
582-60-5
102-32-9
99-50-3
556-50-3
372-75-8
3184-13-2
320-77-4
77-92-97314-32-1
451-13-8
42013-20-7
2867-20-1
407-41-0
462-94-2
1958-8-2
394-31-0

GL Sciences GC Technical Note

Compounds
Alanyl-alanine_2
1,5-Anhydeo-D-glucitol
Homocysteic acid
Tagatose_1
Adenine3-Hydroxyanthranilic acid
Psicose_1
6-Amino-1-methyluracil
Quinic acidHistamine
3-Methoxy-4-hydroxymandelate
Sebacic acid
2-Methylhippurate_2
C19
Psicose_2
Tagatose_2
Imidazole-4-acetate
Fructose_1
Sorbose_1
2-Dehydro-D-gluconate_1
Glucono-1,5-lactone_1
Gluconic acid_1
Sorbose_2
5-Dehydroquinic acid
Allantoin_1
Allose_1
Fructose_2
Indole-3-acetaldehyde_2
4-Hydroxyphenyl pyruvate
Pyridoxine
Allantoin_2
Mannose_1
2-Dehydro-D-gluconate_2
Glucono-1,5-lactone_2
Gluconic acid_2
Galactose_1
Homocitrulline
Glucose_1
Indole-3-acetaldehyde_3
Tyramine
Allose_2
Mannose_2
Lysine_1
Histidinen-Carbamoyl-L-aspartate
3,4-Dihydroxymandelic acid_1
n-Acetyl-L-glutamine
Glucosamine_1
Coniferyl alcohol
Galactose_2
p-Coumaric acid
Glucose_2
Coniferyl aldehyde_1
Tyrosine
1-Hexadecanol
Coniferyl aldehyde_2
Pyridoxamine_1
Pyridoxine_2
Mannitol

Retention
Indices
1874
1877
1877
1881
1886
1886
1888
1889
1891
1892
1893
1895
1897
1900
1900
1901
1902
1905
1906
1907
1910
1911
1911
1911
1912
1913
1915
1916
1918
1919
1920
1921
1924
1925
1925
1927
1928
1932
1933
1934
1939
1940
1941
1942
1942
1944
1946
1947
1949
1951
1951
1953
1955
1958
1961
1962
1963
1963
1969

RetentionTime(s)762.80763.94764.27766.07767.94768.13769.18769.33770.56770.79771.29772.14773.16774.36774.41774.86775.32776.40776.84777.27778.81778.86779.00779.12779.55779.96780.60781.12781.80782.44782.91783.11
784.35784.87784.98785.92786.20788.03788.17788.72791.06791.15791.61792.01
792.14793.06793.97794.35795.35795.82796.11796.82797.71798.80800.39800.59801.23801.23803.78

Cas.No.
154-58-550433-6
87-81-0
73-24-5
548-93-6
551-68-8
2434-53-9
77-95-2
51-45-6
1955-10-7
111-20-6

14403-45-3
57-48-7
87-79-6
3470-37-9
4253-68-3
526-95-4
494211-79-9
97-59-62595-97-3
156-39-8
65-23-6
31103-86-3

26566-61-0
1190-49-4
921-60-8
51-67-2
56-87-1
71-00-1
923-37-5
775-01-9
2490-97-3
3416-24-8
458-35-5
501-98-4
458-36-6
60-18-436653-82-4
524-36-7
69-65-8

GL Sciences GC Technical Note

Compounds
Galactosamine_1
Gallic acid
Glucuronate_1
5-Keto-D-gluconate_1
Cysteric acid
Ascorbic acidSorbitolGlucosamine_2
Glucarate_1
Galactitol
Galactosamine_2
5-Keto-D-gluconate_2
Paraxanthine
Galacturonic acid_1
Epinephrine
Glucuronate_2
n-Acetyl-L-ornithine_1
C20
Glucarate_2
Galacturonic acid_2
Diaminopimelate
Urocanate
S-Benzyl-L-cysteine_1
Pantothenate
n-Acetyl-L-ornithine_2
Palmitoleate
3-Methyladenine
Xanthine
Glucono-1,5-lactone_3
Gluconic acid_32-Deoxyribose-5-phosphateo-Succinyl-L-homoserine
Glucarate_3
1-Methyl histidine_2
Allantoin_3
Kynurenate
n-Acetyl-L-lysine_1
C21
Allantoin_4
Ferulic acid
Dopamine
Sinapyl aldehyde
n-Acetyl-D-glucosamine
Lanthionine
Dopa
Uric acidn-Acetyl-L-lysine_2
Flavanone
Cystathionine_1
Inositol
Arabinose 5-phosphate_1
Pydroxamine
Ribulose 5-phosphate_1
Dethiobiotin_1
Arabinose 5-phosphate_2
Hexadecanoate
Cafferic acid
GuanineRibulose 5-phosphate_2

Retention
Indices
1971
1974
1975
1976
1976
1976
1976
1977
1981
1981
1981
1982
1982
1982
1990
1993
1998
2000
2001
2003
2005
2013
2013
2014
2018
2029
2031
2039
2044
2044
2053
2059
2060
2074
2086
2089
2093
2100
2102
2105
2106
2116
2119
2122
2125
2126
2126
2126
2131
2134
2136
2139
2139
2143
2144
2146
2151
2154
2155

RetentionTime(s)804.43805.83806.37806.50806.62806.64806.70807.25808.56808.76808.88809.20809.22809.33812.65814.05816.15816.82817.42817.98818.95821.97821.98822.55824.20828.70829.19832.73
834.71
834.73
838.23840.63840.93846.76851.69853.02854.54857.31
857.99859.14859.50863.50864.65865.81866.91
867.31867.55867.57869.15870.50871.37872.43872.57873.88874.55875.24877.10878.22878.52

Cas.No.
1772-03-8
149-91-7
6556-12-3
91446-96-7
23537-25-950-81-750-70-4
87-73-0
608-66-2
611-59-6
91510-62-2
51-43-46205-8-9
501-98-4
104-98-3
3054-1-1
137-08-6
373-49-95142-23-4
69-89-6
102916-66-5
1492-23-5

492-27-3
1946-82-3
537-98-4
51-61-64206-58-0
7512-17-6
496-98-0
59-92-7
69-93-2
487-26-3
535-34-2
87-89-889927-09-3
85-87-0108321-99-9
533-48-2
506-12-7
331-39-5
73-40-5

GL Sciences GC Technical Note

Compounds
Methyldopa
5-Sulfosalicylate
C22
2,6-Diamonopurine
Kynurenine
5-Hydroxyindoleactate_1
Elaidic acid
Cystathionine_2
5-Hydroxyindoleactate_2
Tryptamine_1
Cystamine
Octadecanoate
Tryptophan
Sinapic acid
Dethiobiotin_2
Spermidine
Xanthurernate
C23
Cysteine
CystineSinigrin
2-Deoxyuridine_1
C24
Sorbitol 6-phosphate
Dehydroisoandrosterone-3-sulfate
Icosanoic acidp-Aminohippuric acid
3-Hydroxy-kynurenine
2-Deoxyuridine_2
5-Hydroxy-L-tryptophan_1
Biopterin
5-Hydroxy-L-tryptophan_2
Saccharopine_1
Uridine_1
Uridine_2
Serotonin_1
C25
BiotinSerotonin_2
n-Acetyl-D-glucosamine 6-phosphate_1
Salicyl alcohol-β-glucoside
Homocystine
Saccharopine_2
n-Acetyl-D-glucosamine 6-phosphate_22-Deoxyinosine
Ribulose 1,5-bisphosphate_1
Ribulose 1,5-bisphosphate_2
C26
Inosine
S-Benzyl-L-Cysteine_2
Behenic acid
Adenosine
Spermine_1
C27
SucroseXanthosine
Spermine_2
β-Lactose_1
β-Lactose_2

Retention
Indices
2172
2194
2200
2209
2217
2227
2228
2235
2237
2237
2238
2244
2251
2256
2259
2288
2290
2300
2323
2324
2348
2377
2400
2422
2432
2443
2457
2460
2465
2468
2468
2473
2475
2480
2484
2493
2500
2507
2521
2541
2552
2557
2563
2569
2575
2582
2593
2600
2605
2631
2640
2670
2693
2700
2705
2713
2757
2767
2786

RetentionTime(s)885.29893.64896.11899.56902.52906.06906.48908.94909.78909.84910.38912.60915.18916.81917.94928.92929.58933.23941.58941.82950.14960.48968.79976.40979.58983.41
988.13989.21
991.11991.87992.07993.79994.36996.03997.381000.45
1002.98
1005.291009.871016.60
1020.18
1021.931023.981025.901027.761030.111033.721036.151037.871045.98
1048.95
1058.161065.64
1067.80
1069.26
1071.881085.121088.28
1093.92

Cas.No.
41372-08-1
5965-83
1904-98-9
2922-83-0
54-16-0
112-79-8
61-54-1
56-17-7
1957-11-4
73-22-3
530-59-6
124-20-959-00-7
52-90-4
56-89-33952-98-5
951-78-0108392-12-7
78590-17-7
506-30-9
61-78-92147-61-7
4350-9-822150-76-1
997-68-2
58-96-8
153-98-0
58-85-5102029-88-9
870-93-9
890-38-0
14689-84-0
58-63-9
112-85-6
58-61-7
71-44-3
57-50-1
5968-90-1
5965-66-2

GL Sciences GC Technical Note

Compounds
1-Methyladenosine
Spermine_3
C28
Guanosine
2-Deoxyguanosine
Trehalose
Maltose_1
Turanose_1
5-Methylthioadenosine
lignoceric acid
Lactitol
Turanose_2
Maltose_2
Acetoaminophen glucronide
Epicatechin
C29
Sakuranetin_1
Naringenin_1
Maltitol
Melibiose_1
Epigallocatechin
Melibiose_2
Daidzein
Genistein
Sakuranetin_2
Naringenin_2
C30
Apigenine_1
Chlorogenic acid_1
C31
Kaempferol
Apigenin_2
Chlorogenic acid_2
Apigenin_3
C32
Octacosanoic acid
C33
C34
Podophyllotoxin
C35
Raffinose
1-Kestose
Melezitose
C36
Albiflorin
Maltotriose_1
C37
Maltotriose_2
Panose_1
Panose_2
C38

Retention
Indices
2793
2796
2800
2809
2812
2819
2822
2825
2833
2839
2851
2852
2853
2872
2894
2900
2904
2907
2929
2944
2976
2977
2979
2991
2992
2994
3000
3031
3045
3100
3117
3138
3168
3189
3200
3234
3300
3400
3423
3500
3504
3514
3596
3600
3631
3689
3700
3721
3776
3789
3800

RetentionTime(s)1095.98
1096.99
1098.19
1100.841101.861103.851104.881105.711108.151109.811113.421113.75
1113.841119.531126.151127.981129.26
1130.24
1136.95
1141.35
1151.39
1151.69
1152.32
1155.79
1156.35
1156.911158.671168.65
1173.141191.04
1196.781204.241214.541222.071225.75
1238.911263.941306.80
1317.971355.231357.55
1363.291408.871411.28
1431.60
1469.04
1476.021491.62
1533.28
1542.831551.37

Cas.No.
15763-06-1
118-00-3
961-07-9
99-20-7
69-79-4
547-25-1
2457-80-9
557-59-5
585-86-4
120595-80-4
2957-21-3
93602-28-9
585-88-6
5340-95-4
488-66-8
446-72-0

520-36-5
327-97-9

506-48-9
518-28-5
17629-30-0
470-69-910030-67-8
39011-90-0
1109-28-0
33401-87-5

GL Sciences GC Technical Note

Calculating retention indices
Retention indices were able to calculate with n-alkane.

tR – tR(Z)Retention index I = 100 ×n-alkane(Z)tR(Z+1) – t R(Z)

+ 100 × Z

n-alkane(Z+1)
analyte

4.0

Time(min)

5.0

ｔR－ｔR(z)tR= Retention time of analytetR(Z)= Retention time of n-alkane before the analytetR(Z+1) = Retention time of n-alkane after the analyte Z= Carbon number of tR(Z)ｔR(z+1)－ｔR(z)Herewith we especially appreciate Prof. Eiichiro Fukusaki belong to department ofbiotechnology graduate school of engineering, Osaka University, and Dr. Takeshi Banba’s greatcooperation created this library.
GL Sciences disclaims any and all responsibility for any injury or damage which may be causedby this data directly or indirectly. We reserve the right to amend this information or data at any
time and without any prior announcement.

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly. We reserve theright to amend this information or data at any time and without any prior announcement.

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