Kiyotaka Hara

Associate Professor, University of Shizuoka
School of Food and Nutritional Scineces
Graduate School of Nutritional and Environmental Sciences52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan
E-mail: k-hara@u-shizuoka-ken.ac.jp

Education:
B.S.

Life science, Tokyo Institute of Technology, March, 1997

M.S.

Life science, Tokyo Institute of Technology, March, 1999

Ph.D. Life science, Tokyo Institute of Technology, September, 2001

Professional Career:
2001-2003:

Postdoctoral Researcher, ATP System Project, Tokyo Institute of Technology

2003-2006:

Researcher, Bio-frontier Laboratories, Kyowa Hakko Kogyo, Co. Ltd.

2006-2009:

Research Associate, Consolidated Research Institute for Advanced Science andMedical Care, Waseda University

2009-:

Assistant Professor, Organization of Advanced Science and Technology, Kobe
University

2012-:

Associate Professor, Organization of Advanced Science and Technology, Kobe
University

2015-:

Associate Professor, Graduate School of Nutritional and Environmental Sciences,
University of Shizuoka

Research Interests•••••

Energetic Cell Factory
Synthetic Bioengineering
Metabolic Engineering
Fine Chemical Production
Biorefinery

Review:

Kiyotaka Y. Hara, Jyumpei Kobayashi, Ryosuke Yamada, Daisuke Sasaki, Yuki Kuriya, Yoko
Hirono-Hara, Jun Ishii, Michihiro Araki, Akihiko Kondo (2017) Transporter engineering in biomass
utilization by yeast. FEMS Yeast Res. 17(7).

Kiyotaka Y. Hara, Akihiko Kondo (2015) ATP regulation in bioproduction. Microb Cell Factories
14, 198.
Kiyotaka Y. Hara, Michihiro Araki, Naoko Okai, Satoshi Wakai, Tomohisa Hasunuma, Akihiko
Kondo (2014) Development of bio-based fine chemical production through synthetic
bioengineering. Microb Cell Factories 13(1), 173.
Tomohisa Hasunuma, Fumio Okazaki, Naoko Okai, Kiyotaka Y. Hara, Jun Ishii, Akihiko Kondo(2013) A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of theirapplication to consolidated bioprocessing technology. Bioresourse Technology 135, 513-522.
Akihiko Kondo, Jun Ishii, Kiyotaka Y. Hara, Tomohisa Hasunuma, Fumio Matsuda (2013)
Development of microbial cell factories for bio-refinery through synthetic bioengineering. Journalof Biotechnology 163(2), 204-216.

Journal articles:・Hirono-Hara Y, Miyuu Y, Hara KY (2022) Active transglutaminase production from synthetic
whey using engineered Saccharomyces cerevisiae. Bioresource Technology Reports. 19, 101154.

・Kobayashi J, Sasaki D, Hara KY, Hasunuma T, Kondo A (2022) A metabolic engineering of theL-serine biosynthetic pathway improves glutathione production in Saccharomyces cerevisiae.
Microbial Cell Factories. 21(1), 153.

・Toya Y, Hirono-Hara Y, Hirayama H, Kamata K, Tanaka R, Sano M, Kitamura S, Otsuka K,
Abe-Yoshizumi R, Tsunoda SP, Kikukawa H, Kandori H, Shimizu H, Matsuda F, Ishii J, Hara KY(2022) Optogenetic reprogramming of carbon metabolism using light-powering microbial proton
pump systems. Metab Eng. 72, 227-236.

・Hirono-Hara Y, Mizutani Y, Murofushi K, Iwahara K, Sakuragawa S, Kikukawa H, Hara KY(2022) Effect of spent coffee grounds extract on astaxanthin production by Xanthophyllomyces
dendrorhous. Bioresorce Technology Reports. 17, 100953.

Kikukawa H, Shimizu C, Hirono-Hara Y, Hara KY (2022) Effect of ethanol on astaxanthin and fatty
acid production in the red yeast Xanthophyllomyces dendrorhous. Journal of Applied Microbiology.
132, 2034-2041.

Hara KY, Yagi S, Hirono-Hara Y, Kikukawa H (2021) A method of solubilizing and concentratingastaxanthin and other carotenoids. Marine Drugs. 19, 462.

Hirono-Hara Y, Mizutani Y, Murofushi K, Iwahara K, Sakuragawa S, Kikukawa H, Hara KY (2021)
Glutathione fermentation by Millerozyma farinosa using spent coffee grounds extract and seawater.
Bioresorce Technology Reports. 15, 100777.

Kikukawa H, Shimizu C, Hirono-Hara Y, Hara KY (2021) Screening of plant oils promoting growthof the red yeast Xanthophyllomyces dendrorhous with astaxanthin and fatty acid production.
Biocatalysis and Agricultural Biotechnology. 35, 102101.

Kikukawa H, Okaya T, Maoka T, Miyazaki M, Murofushi K, Kato T, Hirono-Hara Y, Katsumata M,
Miyahara S, Hara KY (2021) Carotenoid nostoxanthin production by Sphingomonas sp. SG73
isolated from deep sea sediment. Marine Drugs. 19(5), 274.

Hirono-Hara Y, Yui M, Hara KY (2021) Production of transglutaminase in glutathione-producing
recombinant Saccharomyces cerevisiae. AMB Express. 11, 13.

Hara KY, Kageyama Y, Tanzawa N, Hirono-Hara Y, Kikukawa H, Wakabayashi K (2020)
Development of astaxanthin production from citrus peel extract using Xanthophyllomyces
dendrorhous. Environ. Sci. Pollut. Res. 28, 12640-12647.

Imai Y, Inoshita T, Meng H, Shiba-Fukushima K, Hara KY, Sawamura N, Hattori N. (2019)
Light-driven activation of mitochondrial proton-motive force improves motor behaviors in a
Drosophila model of Parkinson's disease. Commun Biol. 2, 424.

Hara KY, Saito M, Kato H, Morikawa K, Kikukawa H, Nomura H, Fujimoto T, Hirono-Hara Y,
Watanabe S, Kanamaru K, Kondo A. (2019) 5-Aminolevulinic acid fermentation using engineered
Saccharomyces cerevisiae. Microb Cell Fact. 18 (1), 194.

Prima A, Hara KY, Djohan AC, Kashiwagi N, Kahar P, Ishii J, Nakayama H, Okazaki F, Prasetya B,Kondo A, Yopi, Ogino C. (2017) Glutathione production from mannan-based bioresource bymannanase/mannosidase expressing Saccharomyces cerevisiae. Bioresour Technol. 245 (Pt B),1400-1406.

Kobayashi J, Sasaki D, Hara KY, Hasunuma T, Kondo A. (2017) Enzymatic improvement of
mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces
cerevisiae. Microb Cell Fact, 16(1), 44.

Yamamoto K, Hara KY (equal contribution), Morita T, Nishimura A, Sasaki D, Ishii J, Ogino C,Kizaki N, Kondo A. (2016) Enhancement of astaxanthin production in Xanthophyllomyces
dendrorhous by efficient method for the complete deletion of genes. Microb Cell Fact. 15(1), 155.

Ishii J, Okazaki F, Djohan AC, Hara KY, Asai-Nakashima N, Teramura H, Andriani A, Tominaga M,Wakai S, Kahar P, Yopi, Prasetya B, Ogino C, Kondo A. (2016) From mannan to bioethanol: cellsurface co-display of -mannanase and -mannosidase on yeast Saccharomyces cerevisiae.
Biotechnol Biofuels. 9(1), 188.

Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki
M, Hara KY, Shimatani Z, Kondo A. (2016) Targeted nucleotide editing using hybrid prokaryotic
and vertebrate adaptive immune systems. Science. 353 (6305).

Sasaki K, Hara KY (equal contribution), Kawaguchi H, Sazuka T, Ogino C, Kondo A. (2016)

Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomycescerevisiae. J Biosci Bioeng 121, 96-100.

Hara KY, Aoki N, Kobayashi J, Kiriyama K, Nishida K, Araki M, Kondo A. (2015) Improvementof oxidized glutathione fermentation by thiol redox metabolism engineering in Saccharomyces
cerevisiae. Appl Microbiol Biotechnol 99, 9771-9778.

Inokuma K, Ishii J, Hara KY, Mochizuki M, Hasunuma T, Kondo A. (2015) Complete Genome
Sequence of Kluyveromyces marxianus NBRC1777, a Nonconventional Thermotolerant Yeast.
Genome Announc. 3(2), e00389-15.
Ye X, Morikawa K, Ho SH, Araki M, Nishida K, Hasunuma T, Hara KY (Corresponding author),Kondo A. (2015) Evaluation of genes involved in oxidative phosphorylation in yeast by developing
a simple and rapid method to measure mitochondrial ATP synthetic activity. Microb Cell Fact 14,56.
Hideo Kawaguchi, Hiroshi Teramura, Kouji Uematsu, Kiyotaka Y. Hara, Tomohisa Hasunuma, Ko
Hirano, Takashi Sazuka, Hidemi Kitano, Yota Tsuge, Prihardi Kahar, Satoko Niimi-Nakamura,Ken-Ichi Oinuma, Naoki Takaya, Shigemitsu Kasuga, Chiaki Ogino, and Akihiko Kondo (2015)
Phenyllactic acid production by simultaneous saccharification and fermentation of pretreatedsorghum bagasse. Bioresource Technology, 182, 169-178.
Kiyotaka Y. Hara, Toshihiko Morita, Masao Mochizuki, Keisuke Yamamoto, Chiaki Ogino,Michihiro Araki, Akihiko Kondo (2014) Development of a multi-gene expression system in
Xanthophyllomyces dendrorhous. Microbial Cell Factories 13(1), 175.
Michihiro Araki, Robert Sidney Cox III, Hiroki Makiguchi, Teppei Ogawa, Takeshi Taniguchi,Kohei Miyaoku, Masahiko Nakatsui, Kiyotaka Y. Hara, Akihiko Kondo (2014) M-path: a compass
for navigating potential metabolic pathways. Bioinformatics. 31(6), 905-911.
Kiyotaka Y. Hara, Katsuhiko Morita, Yusuke Endo, Masao Mochizuki, Michihiro Araki, AkihikoKondo (2014) Evaluation and screening of efficient promoters to improve astaxanthin production in
Xanthophyllomyces dendrorhous. Applied Microbiology and Biotechnology 98(15), 6787-6793.
Shih-Hsin Ho, Akihito Nakanishi, Xiaoting Ye, Jo-Shu Chang, Kiyotaka Hara, Tomohisa Hasunuma,Akihiko Kondo (2014) Optimizing biodiesel production in marine Chlamydomonas sp.JSC4
through metabolic profiling and an innovative salinity-gradient strategy. Biotechnology for Biofuel7, 97.

Kentaro Kiriyama, Kiyotaka Y. Hara (equal contribution), Akihiko Kondo (2013) Oxidized
glutathione fermentation using Saccharomyces cerevisiae engineered for glutathione metabolism.
Applied Microbiology and Biotechnology 97(16), 7399-7404.
Kiyotaka Y. Hara, Takeyoshi Wada, Kuniki Kino, Toru Asahi, Naoya Sawamura (2013)
Construction of photoenergetic mitochondria in cultured mammalian cells. Scientific Reports 3,1635.
Kentaro Kiriyama, Kiyotaka Y. Hara (equal contributin), Akihiko Kondo (2012) Extracellularglutathione fermentation using engineered Saccharomyces cerevisiae expressing novel glutathione
exporter. Applied Microbiology and Biotechnology 96(4), 1021-1027.
Jun Ishii, Miyuki Moriguchi, Kiyotaka Y. Hara, Seiji Shibasaki, Hideki Fukuda, Akihiko Kondo(2012) Improved identification of agonist-mediated Gαi-specific human G-protein-coupled receptor
signaling in yeast cells by flow cytometry. Analytical Biochemistry 426(2), 129-133.
Kiyotaka Y. Hara, Songhee Kim, Kentaro Kiriyama, Hideyo Yoshida, Syogo Arai, Jun Ishii, ChiakiOgino, Hideo Fukuda, Akihiko Kondo (2012) An energy-saving glutathione production methodfrom low-temperature cooked rice using amylase-expressing Saccharomyces cerevisiae.
Biotechnology Journal 7(5), 686-689.
Kiyotaka Y. Hara, Kentaro Kiriyama, Hideo Nakayama, Akihiko Kondo (2012) Improvement ofglutathione production by metabolic engineering the sulfate assimilation pathway of Saccharomyces
cerevisiae. Applied Microbiology and Biotechnology 94(5), 1313-1319.
Kiyotaka Y. Hara, Songhee Kim, Hideyo Yoshida, Kentaro Kiriyama, Takashi Kondo, Naoko Okai,N, Chiaki Ogino, Hideo Fukuda, Akihiko Kondo (2012) Development of a glutathione production
process from proteinaceous biomass resources using protease-displaying Saccharomyces cerevisiae.
Applied Microbiology and Biotechnology 93(4), 1495-1502.
Hideyo Yoshida, Kiyotaka Y. Hara (equal contribution), Kentro Kiriyama, Hideo Nakayama, Fumio
Matsuda,Chiaki Ogino, Hideo Fukuda, Akihiko Kondo (2011) Enzymatic glutathione production
using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst., AppliedMicrobiology and Biotechnology 91(4), 1001-1006.
Kiyotaka Y. Hara, Rie Suzuki, Toshiharu Suzuki, Masasuke Yoshida, Kuniki Kino (2011) ATP
photosynthetic vesicles for light-driven bioprocesses. Biotechnology Letters 33(6), 1133-1138.

Hideyo Yoshida, Shogo Arai, Kiyotaka Y. Hara, Ryosuke Yamada, Chiaki Ogino, Hideo Fukuda,Akihiko Kondo (2011) Efficient and direct glutathione production from raw starch using
Saccharomyces cerevisiae. Applied Microbiology and Biotechnology 89(5), 1417-1422.Kiyotaka Y.
Hara, Natsuka Shimodate, Yasutaka Hirokawa, Mikito Ito, Tomoya Baba, Hirotada Mori, Hideo
Mori (2009) Glutathione production by efficient ATP-regenerating Escherichia coli mutants. FEMS
Microbiology Letters 297(2), 217-214.
Kiyotaka Y. Hara, Natsuka Shimodate, Mikito Ito, Tomoya Baba, Hirotada Mori, Hideo Mori
(2009) Systematic genome-wide scanning for genes involved in ATP generation in Escherichia coli.
Metabolic Engineering 11(1), 1-7.
Kiyotaka Y. Hara, Hideo Mori (2006) An efficient method for quantitative determination of cellular
ATP synthetic activity. Journal of Biomolecular Screening 11(3), 310-317.
Katsuya Shimabukuro, Ryohei Yasuda, Eiro Muneyuki, Kiyotaka Y. Hara, Kazuhiko Kinosita, Jr,Masasuke Yoshida (2003) Catalysis and rotation of F1 motor: cleavage of ATP at the catalytic site
occurs in 1 ms before 40 degree substep rotation. Proceedings of National Academy of Sciences ofthe United States of America 100(25),14731-14736.
Sakurako Ono, Kiyotaka Y. Hara, Jun Hirao, Tadashi Matsui, Hiroyuki Noji, Masasuke Yoshida,
Eiro Muneyuki (2003) Origin of apparent negative cooperativity of F(1)-ATPase., Biochimica etBiophysica Acta -Bioenergetics 1607(1), 35-44.
Yoko Hirono-H, Hiroyuki Noji, Masaya Nishiura, Eiro Muneyuki, Kiyotaka Y. Hara, Ryohei
Yasuda, Kazuhiko Kinosita, Jr, Masasuke Yoshida (2001) Pause and rotation of F(1)-ATPase duringcatalysis. Proceedings of National Academy of Sciences of the United States of America
98(24),13649-13654.
Kiyotaka Y. Hara, Yasuyuki Kato-Yamada, Yuji Kikuchi, Toru Hisabori, Masasuke Yoshida (2001)
The role of the betaDELSEED motif of F1-ATPase: propagation of the inhibitory effect of theepsilon subunit. Journal of Biological Chemistry 276(26), 23969-23973.
Kiyotaka Y. Hara, Hiroyuki Noji, Dirk Bald, Ryohei Yasuda, Kazuhiko Kinosita, Jr., MasasukeYoshida (2000) The role of the DELSEED motif of the beta subunit in rotation of F1-ATPase.
Journal of Biological Chemistry 275(19), 14260-14263.
Toyoki Amano, Tadashi Matsui, Eiro Muneyuki, Hiroyuki Noji, Kiyotaka Hara, Masasuke Yoshida,
Toru Hisabori (1999) alpha3beta3gamma complex of F1-ATPase from thermophilic Bacillus PS3
can maintain steady-state ATP hydrolysis activity depending on the number of non-catalytic sites.

Biochemical Journal 343(1), 135-138.
Book (English):Kiyotaka Y. Hara (2009) Methods in Molecular Biology: Methods and Protocols, Reverse ChemicalGenetics (Section 2.7. Permeable Cell Assay: a method for high-throughput measurement ofcellular ATP synthetic activity), Methods in Molecular Biology (Humana Press) 577, 251-257.