GC Technical Note

Simple Concentration and Analysis of Cabbage Spoilage
Odor –Use of Sorptive Media Mono Trap

GT070
GL Sciences Inc.

MonoTrap RGC18TD , a simple concentration tool was used to screen for volatile components in spoiled
cabbage. At the same time sensory evaluation was performed on these odors.

Pretreatment procedure
Cabbage

And chicken, place 25 g in 100 mL vials

Decay

Allow to stand at 60 ˚C for a period of time
(3 to 7 days) and rot
60 ˚C for 3 days
Immediately after cutting

Collection (HS)2 MonoTrap RGC18TDAt room temperature for 3 hConditions
SystemColumn

TD-GC-MS
OdorCol.Temp.
Carrier Gas: GC-MS-Thermal Desorption: InertCap Pure-WAX
0.25 mm I.D. x 60 m df = 0.25 μm: 40 ˚C (5 min) - 6 ˚C/min - 250 ˚C: He 1 mL/min (constant flow)

Desorb Temp.
TimeFlowSplit
Cryo Trapping
Injection Temp.
Detection: 200 ˚C
: 5 min: 5 mL/min: Splitless: -150 ˚C: 250 ˚C
: MS Scan (m/z : 28.5 - 600)

Odor-sniffing device
Sniffing pot (OP275)

Comparison of volatile constituents with advancing spoilage(x10,000,000)

1.0

0.9

0.8

0.7

0.6

0.5

At 60 ˚C for 7 days

0.4

0.3

0.2

At 60 ˚C for 3 days

0.1

0.0

Immediately after cutting

-0.1
5.0

7.5

10.0

12.5

15.0

17.5

Time (min)

20.0

22.5

25.0

27.5

30.0

GL Sciences GC Technical Note
The outlet of the capillary column was split so that GC/MS and GC/O (GC/Olfactometry) could be measured
simultaneously. Due to the splitting of the flow, the detection sensitivity is approx. 1/10th of normal. S and N
based compounds can also be well detected by the effects of graphite carbon contained in the MonoTrap
RGC18TD.
collector
Volatile components of cabbage at 60 ˚C for 7 days

Time (min)

Peak
No.
1.
2.
3.
4.
5.
6.
7.

Dimethyl disulfide
Hexanal2-Heptanone
1-Butanol, 2-methyl1-Butanol, 3-methyl1-Pentanol
Hexanenitrile

8.

2-Butanone, 3-hydroxy-9.
10.

Dimethyl trisulfide
1-Hexanol

CompoundAssessment of Peakodor
No.
Rotten egg
11.
Greenly
12.
13.
Raw garbage 14.
Bitter15.
Greenly
16.
Bitter
17.
18.
Putrid odor 19.
Pungent odor

CompoundAcetic acid
1-Octen-3-ol e alcohol
Hexane, 1-nitroBenzaldehyde
4-Hexen-1-olBenzonitrile
S-Methyl methanethiosulphonate(2,6,6-Trimethyl-2-hydroxycyclohexylidene)acetic acid lactone
IndoleAssessmentof odor
SourMetal

BitterPutrid odor

Fecal

* Standard samples are not used for qualitative analysis. Results from a library search.
Red letters: strong odor

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly.
We reserve the right to amend this information or data at any time and without any prior announcement.

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