GC Technical Note

Purity Test of Calcium Polystyrene Sulfonate, with reference to theJapanese Pharmacopoeia, 16th Edition - Comparisons of
Chromosorb Series and Uniport Series of Siliceous Soil Support

GT083
GL Sciences Inc.

Calcium polystyrene sulfonate is used as a
cardiovascular drug. It reduces potassium in theblood and is used to treat hyperkalemia caused bykidney failure. In this study, a packed column wasused for analysis with reference to purity test (4)described in the Japanese Pharmacopoeia, 16thedition.

Chromosorb series and Uniport series siliceousearth carriers were evaluated and compared. This
report demonstrates that both supports satisfy therequirements of the test method.

Example: Measurement of standard
Chromosorb WAW DMCS
(1 μg/mL)

1. Styrene(1 μg/mL)

10000

10000

uVolts20000

uVolts
20000

30000

30000

1. Styrene

Uniport HP

100102468Time (min)10120142468Time (min)101214

Example: Measurement of performance test standard of the system
Chromosorb WAW DMCS
(10 μg/mL)

1. Styrene(10 μg/mL)

uVolts40000
60000

uVolts
40000
60000

80000

80000

1. Styrene

Uniport HP20000

10020000

102468Time (min)10121402468Time (min)101214Blank
Chromosorb WAW DMCS

0020000

20000

uVolts40000
60000

uVolts
40000
60000

80000

80000

Uniport HP

02468Time (min)10121402468Time (min)101214

GL Sciences GC Technical Note

Measurement conditions
Conditions
SystemColumn

Injection: GC - FID: PEG 20M 15 %
Chromosorb WAW DMCS 80/100
Uniport HP 80/100
SUS 2 m x 3 mm I.D.
: 90 ˚C: N2 180 kPa

Col. Temp.
Carrier Gas

Injection Vol.
Detection
Sample: Direct250 ˚C: 5 μL: FID Auto Range
250 ˚C: Standard
Analyte in Acetone

Measurement

Requirements for System Suitability
1．When the test is made with the system performance test standard, thenumber of theoretical plates and symmetry factor of the styrene peak are
not less than 800 and 0.8 to 1.2, respectively.
2．When the test is repeated 6 times with the test standard, the relative
standard deviation of the peak height of styrene is not more than 5%.

Results
Table 1. System performance test results

Number oftheoretical plates
Symmetry factor

Chromosorb WAW DMCS

Uniport HP35023715

1.05

1.05

Table 2. System repeatability test results

Chromosorb WAW DMCS

Uniport HP

11455

1419

21409

1400

31439

1473

41360

1441

51398

1361

61450

1397

Mean

1419

1415

Standard deviation
Relative standard
deviation (%)

36.5

38.8

2.58

2.74

GL Sciences GC Technical Note
Introduction of diatomaceous earth carriers
The Japanese Pharmacopoeia contains several tests: “Siliceous earth packings for gas chromatography with XXX%
liquid phase.”The two most commonly used diatomaceous earth carriers that were used in these tests are describedhere.

Uniport series
Uniport B (sintered after washing + acid-treated product)
Special treatment removes metals from the surface of the support, making it easy to use without catalytic
activity.
It can be used with a wide range of liquid phases as well as polyglycols and polyesters.
Uniport HP (sintering, acid treatment, and silane treatment after washing)
Uniport B is specifically silanized. It is the most inert carrier for silanization.
It has good compatibility with silicon systems and is suitable to the sample that is easy to adsorb anddisassemble.

Chromosorb series
Chromosorb WAW (sintered after washing + acid-treated product)
Chromosorb WAW DMCS (sintered after washing + acid treatment + silane treatment)

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly.
We reserve the right to amend this information or data at any time and without any prior announcement.

GL Sciences, Inc. JapanGL Sciences B.V.

GL Sciences, Inc. USA

GL Sciences (ShangHai) Ltd.

22-1 Nishishinjuku 6-Chome
Shinjuku-ku, Tokyo,163-1130, Japan
Phone: +81-3-5323-6620
Fax: +81-3-5323-6621
Email: world@gls.co.jp
Web: www.glsciences.com

De Sleutel 95652 AS Eindhoven
The Netherlands
Phone: +31 (0)40 254 95 31
Email: info@glsciences.eu
Web: www.glsciences.eu

4733 Torrance Blvd. Suite 255
Torrance, CA 90503
Phone: 310-265-4424
Fax:
310-265-4425
Email: info@glsciencesinc.com
Web: www.glsciencesinc.com

Tower B, Room 2003,
Far East International Plaza,NO,317 Xianxia Road,
Changning District.
Shanghai, China P.C. 200032
Phone: +86 (0)21-6278-2272
Email: contact@glsciences.com.cn
Web: www.glsciences.com.cn

International Distributors
Visit our Website at www.glsciences.com/distributors