Anti-BPAG1（BP230）antibody, mouse monoclonal (279)
Product code

70-365Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

Immunogen

Native BP230 from bovine cornea

Isotype

Mouse IgG1κ

Reactivity

Bullous pemphigoid (BP) antigen 1 (Human, Rat, Rabbit, Bovine, Porcine)Special notes

N/A

Application

1. Western blotting: x1/1,000 (Fig.1)
2. Immunofluorescence microscopy x1/250-500 (Fig.2,3)

Background

BPAG1 (Bullous pemphigoid antigen I), also known as dystonin (DST) or BP230,
is a member of the plakin protein family expressing in various tissues. BPAG1
plays crucial roles in numerous biological processes, such as cytoskeleton
organization, cell polarization, cell adhesion and cell migration．It was first
identified as an autoantigen in patients with bullous pemphigoid (BP), anautoimmune skin disease. BP typically occurs in older adults and may involvethe formation of large, fluid-filled blisters (bullae) in the space between theepidermal and dermal skin layers.

Data Link

UniProtKB: Q03001 (DYST_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.

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Data Images: 70-365 Anti-BPAG1（BP230）antibody, mouse monoclonal (279)

Fig.1 Western blot analysis of 279 antibody
Whole cell lysate prepared from FRSK (fetal rat skin keratinocyte) cells was stained with CBB andimmunoblotted with 279 antibody at 1:1,000 dilution. The HRP-conjugated goat anti-mouse IgG wasused as the second antibody. The 279 antibody recognized BPAG1 (Bullous Pemphigoid antigen 1) asa band at approximately 230 kDa (arrow), as visualized using a chemiluminescent detection with
EzWestLumi plus kit (ATTO, Tokyo, Japan).

Fig.2

Immunofluorescence microscopy of human skin

A frozen acetone-fixed human skin section was stained with 279 antibody (1:500 dilution). The FITCconjugated goat anti-mouse IgG was used as the second antibody. The antibody revealed the locationof BPAG1 at the dermal-epidermal junction (arrows). E: epidermis, D: dermis. Bar = 50 m.

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Fig.3

Immunofluorescence microscopy of cultured FRSK cells

Methanol-fixed FRSK (fetal rat skin keratinocyte) cells were stained with 279 antibody (1:500
dilution). The FITC-conjugated goat anti-mouse IgG was used as the second antibody. The antibody
detected typical dotted patterns of hemidesmosomes. Bar = 20 m.
Reference:
1. Owaribe K, Nishizawa Y, Franke WW. Isolation and characterization of hemidesmosomes frombovine corneal epithelial cells. Exp Cell Res. 192:622-630, 1991.
2. Okumura M, Yamakawa H, Ohara O, Owaribe K. Novel alternative splicings of BPAG1 (bullouspemphigoid antigen 1) including the domain structure closely related to MACF (microtubule actin
cross-linking factor). J Biol Chem. 277:6682-6687, 2002.
3. Hirako Y, Usukura J, Uematsu J, Hashimoto T, Kitajima Y, Owaribe K. Cleavage of BP180, a 180kDa bullous pemphigoid antigen, yields a 120-kDa collagenous extracellular polypeptide. J BiolChem. 273:9711-9717, 1998

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