Product datasheet

DAB Substrate Kit ab64238
7 Abreviews 380 References 画像数 3

製品の概要
製品名

DAB Substrate Kit

製品の概要

DAB substrate kit ab64238 is used for IHC staining using HRP / peroxidase enzyme fordetection. Reagent is stable for up to 6 hours after mixing the two components.
DAB (3,3'Diaminobenzidine) is the most commonly used chromogen for immunohistochemicalstaining. In the presence of HRP / peroxidase, DAB produces a brown precipitate that is insoluble
in alcohol.
This product is a two component form consisting of a liquid, refrigerator stable DAB Chromogenand DAB Substrate.

IHC protocol suitable for use with DAB substrate kit:
For frozen sections, skip steps 1 and 2.
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat
sections. Wash 3 times in buffer.
3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.
4. Apply protein block (or normal serum from same species as secondary antibody) andincubate for 5 minutes at room temperature to block nonspecific background staining. Wash oncein buffer.
5. Apply primary antibody in antibody diluent and incubate.
6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP
polymer secondary antibody and skip step 7). Wash 4 times in buffer.
7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.
18. Rinse 4 times in buffer. Place slide in DAB substrate ab64238 or AEC substrate andincubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.
9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.
特記事項

Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification,visualization, counterstaining, and mounting in the IHC kits and reagents guide.

アプリケーション

適用あり : IHC-P

製品の特性
保存方法

Store at +4°C. Please refer to protocols.

内容60 ml

125 ml50x DAB Chromogen

1 x 2ml1 x 4ml

DAB substrate

1 x 60ml

1 x 125ml

関連性

3,3' Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining. In
the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble inalcohol.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab64238の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション

IHC-P

Abreviews

特記事項

Use at an assay dependent dilution.

画像2

Immunohistochemical analysis of healthy and colitis mouse colon
sections (untreated and treated with enoxaparin) labeling claudin-4
with ab15104 at 1/200. ab7090, anti-rabbit immunoglobulin G
conjugated to horseradish peroxidase (HRP) at 1/300 was used as
the secondary antibody. ab64238 at 1/50 was used to develop thehistological signal.
Antigen retrieval was performed by incubating the sections for 10minutes at 97°C in 1 mM EDTA buffer, pH 8.0 or 10 mM citratebuffer, pH 6.0.
Control, C; untreated colitis, DSS; oral enoxaparin, OE;
intraperitoneal injection of enoxaparin, IPE.
Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections)
Lean QY et al. PLoS One (2015) Reproduced under theCreative Commons license
http://creativecommons.org/licenses/by/4.0/

Immuno-staining of formalin–fixed and paraffin-embedded liver
sections with ab64238. Sections were obtained from control(saline) and treated with CCl4+CT-N and CCl4+AD-N (left) andantibody control (right).

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections)
Kumar P et al. PLoS One (2014) Reproduced under theCreative Commons license
http://creativecommons.org/licenses/by/4.0/

3Immunohistochemical analysis of mouse lung. Samples were fixedin 10% buffered formalin, paraffinized and sliced at 1.5 µm thick.
Antigen retrieval was performed using ab64214 for thedeparaffinized slices. Sections were blocked with 2% normal goat
serum, PBS(-) and 0.1% Tween20. They were then incubated withthe primary antibodies for 1 hour at 4°C and with secondary
antibodies for 30 minutes at room temperature. The avidin-biotinperoxidase complex method with peroxidase streptavidin and theDAB substrate kit ab64238 was performed.
(A) A resected lung from a mouse sacrificed 28 days after SCL
injection.
Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections)Jujo T et al. PLoS One (2014) Reproduced under theCreative Commons license
http://creativecommons.org/licenses/by/4.0/(B) Haemotoxylin and Eosin staining showing the tumor composedof a central area with necrosis and a peripheral zone filled withSCLs.
(C) Immunohistochemical staining for MMP-14 showing positive
expression of MMP-14 in the peripheral zone of the tumor andnegative in central zone.
(D) Immunohistochemical staining weak staining of MMP-2 in theperipheral zone.

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