Product datasheet

Anti-VDAC1/Porin + VDAC2 + VDAC3 antibody – Mitochondrial
Loading Control ab15895
35 Abreviews 398 References 画像数 6
製品の概要
製品名

Anti-VDAC1/Porin + VDAC2 + VDAC3 antibody – Mitochondrial Loading Control

製品の詳細

Rabbit polyclonal to VDAC1/Porin + VDAC2 + VDAC3 - Mitochondrial Loading Control

由来種

Rabbit特異性

This antibody detects VDAC1, VDAC2 and VDAC3.

アプリケーション

適用あり : IHC-P, WB, ICC/IF

種交差性

交差種 : Mouse, Rat, Chicken, Cow, Dog, Human, Chinese hamster
交差が予測される動物種 : Rabbit, Pig, Zebrafish

免疫原Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

ポジティブ･コントロール

WB: HeLa, A431,Jurkat, PC12, Rat kidney and HEK293 whole cell lysates, Mouse heart, kidney,skeletal muscle, spinal cord tissue lysate and rat brain tissue lysate. ICC/IF: HeLa cells. IHC-P:
FFPE normal human heart tissue.

特記事項

The Life Science industry has been in the grips of a reproducibility crisis for a number of years.
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies
and knockout edited cell lines for gold-standard validation. Please check that this product meets
your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or
contact our Support team ahead of purchase. Recommended alternatives for this product can befound below, along with publications, customer reviews and Q&As

製品の特性
製品の状態

Liquid

保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.

バッファー

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising
agent. If you would like information about the formulation of a specific lot, please contact our1scientific support team who will be happy to help.
精製度

Immunogen affinity purified

ポリ /モノ

ポリクローナルアイソタイプ

IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab15895の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション

特記事項

Abreviews

IHC-P(10)Use a concentration of 0.1 µg/ml. Perform heat mediated antigen
retrieval with citrate buffer pH 6 before commencing with IHC
staining protocol.

WB

(20)

Use a concentration of 1 µg/ml. Detects a band of approximately
31 kDa (predicted molecular weight: 31 kDa).
Abcam recommends using BSA as the blocking agent.

ICC/IF(1)

Use a concentration of 5 µg/ml.

ターゲット情報
細胞内局在

VDAC1/Porin: Mitochondrion outer membrane. Cell membrane. VDAC2: Mitochondrion outermembrane. VDAC3: Mitochondrial outer membrane.

画像2

IHC image of VDAC1/Porin + VDAC2 + VDAC3 staining in asection of formalin-fixed paraffin-embedded normal human heart
performed on a Leica Biosystems BOND® RX instrument. The
section was pre-treated using heat mediated antigen retrieval withsodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins.
The section was then incubated with ab15895, 0.1 μg/ml, for 15mins at room temperature and detected using an HRP conjugatedcompact polymer system. DAB was used as the chromogen. The
section was then counterstained with haematoxylin and mountedwith DPX. The inset secondary-only control image is taken from an
identical assay without primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-

For other IHC staining systems (automated and non-automated)

embedded sections) - Anti-VDAC1/Porin + VDAC2 +customers should optimize variable parameters such as antigen

VDAC3 antibody – Mitochondrial Loading Control

retrieval conditions, primary antibody concentration and antibody(ab15895)

incubation times.
Tissue obtained from the Human Research Tissue Bank, supportedby the NIHR Cambridge Biomedical Research Centre.

All lanes : Anti-VDAC1/Porin + VDAC2 + VDAC3 antibody –Mitochondrial Loading Control (ab15895) at 1 µg/ml

Lane 1 : Hela Nuclear lysate
Lane 2 : Hela cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : Hela Nuclear lysate with Human VDAC1/Porin peptide(ab16131) at 1 µg/ml

Lane 7 : Hela cell lysate with Human VDAC1/Porin peptide
Western blot - Anti-VDAC1/Porin + VDAC2 +(ab16131) at 1 µg/ml

VDAC3 antibody – Mitochondrial Loading Control

Lane 8 : A431 cell lysate with Human VDAC1/Porin peptide(ab15895)(ab16131) at 1 µg/ml

Lane 9 : Jurkat cell lysate with Human VDAC1/Porin peptide(ab16131) at 1 µg/ml

Lane 10 : HEK293 cell lysate with Human VDAC1/Porin peptide(ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.

SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000
dilution
Developed using the ECL technique.
3Performed under reducing conditions.

Predicted band size: 31 kDa
Observed band size: 31 kDa

All lanes : Anti-VDAC1/Porin + VDAC2 + VDAC3 antibody –Mitochondrial Loading Control (ab15895) at 1 µg/ml

Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Mouse skeletal muscle tissue lysate - total protein(ab29711)

Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole
Cell Lysate
Western blot - Anti-VDAC1/Porin + VDAC2 +VDAC3 antibody – Mitochondrial Loading Control

Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
Lane 7 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)(ab15895)

Lysates/proteins at 10 µg per lane.

SecondaryAll lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at
1/10000 dilution
Performed under reducing conditions.

Predicted band size: 31 kDa
Observed band size: 31 kDa

4ab15895 staining VDAC1/Porin + VDAC2 + VDAC3 in HeLa cells.
The cells were fixed with 100% methanol (5 min), permeabilizedwith 0.1% PBS-Triton X-100 for 5 minutes and then blocked with
1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tweenfor 1h. The cells were then incubated overnight at 4°C with ab15895
at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin- Loading Control. Cells were then incubated with ab150081, Goatpolyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor®
488), pre-adsorbed at 1/1000 dilution (shown in green) andab150120, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown inImmunocytochemistry/ Immunofluorescence - Anti-pseudocolour red). Nuclear DNA was labelled with DAPI (shown in

VDAC1/Porin + VDAC2 + VDAC3 antibody –blue).

Mitochondrial Loading Control (ab15895)

Image was acquired with a high-content analyser (Operetta CLS,Perkin Elmer) and a maximum intensity projection of confocal
sections is shown.

All lanes : Anti-VDAC1/Porin + VDAC2 + VDAC3 antibody –Mitochondrial Loading Control (ab15895) at 1 µg/ml

Lane 1 : Chicken liver cell lysate
Lane 2 : CHO K1 cell lysate
Lane 3 : MDCK cell lysate
Lane 4 : Chicken liver cell lysate with Human VDAC1/Porin peptide(ab16131) at 1 µg/ml

Lane 5 : CHO K1 cell lysate with Human VDAC1/Porin peptide(ab16131) at 1 µg/ml
Western blot - Anti-VDAC1/Porin + VDAC2 +Lane 6 : MDCK cell lysate with Human VDAC1/Porin peptide

VDAC3 antibody – Mitochondrial Loading Control(ab16131) at 1 µg/ml(ab15895)

Lysates/proteins at 20 µg per lane.

SecondaryAll lanes : Alexa fluor goat polyclonal anti-Rabbit IgG at 1/10000
dilution
Developed using the ECL technique.
Performed under reducing conditions.

Predicted band size: 31 kDa5Western blot image using the Optiblot Reducing Electrophoresis Kit- 10 x 10 cm (4-20%) (ab119220) with thePrism Ultra Protein Ladder (ab116028) 5µl used. We recommend
using our ECL substrate kit (ab65623) .
20ug of Lysate per lane and detection using ab15895 diluted to1ug/ml.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: HEK293 cell lysate
Lane 5: HepG2 cell lysate.
Western blot - Anti-VDAC1/Porin + VDAC2 +VDAC3 antibody – Mitochondrial Loading Control(ab15895)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"

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