Sep. 13rd, 2019
Protein Crystal Analysis Division, JASRI

Please carefully read the following instructions. If you have any question,please contact us (bioxtal@spring8.or.jp).

1.

Beamline alignment in the morning
The first 30 minutes of the beamtime starting in the morning will be used for thebeamline alignment. This is needed for the stable use of beamlines. We willappreciate your cooperation.

2.

Caution for the staff supporti) Beamline staff will not attend at the beginning of the 2nd and 3rd shift inprinciple if the user is not a novice.
ii) Contact beamline staff prior to your beamtime, if you need an instruction whenyour beamtime is allocated in 2nd shift or later.
iii) We expect you to conduct experiment with experienced person in your lab
when your beam time is allocated at midnight or weekend. We appreciate yourcooperation to reduce the burden of the staff.

5.

Sample changer
We ask all users to use the sample changer which use Uni-Puck as a cassette. If
you don’t have any Uni-Puck, you can use our Uni-Puck.
If you want to mount samples by hand at BL41XU, please contact us prior to yourbeamtime. It is impossible at BL45XU to mount samples by hand.

6.

Helium cryo-cooler at BL41XUYou can use Helium cryo-cooler which can cools samples down to 20 K. If you
want to use it, please contact beamline staff at least two month before yourbeamtime. We have difficulty to buy Helium gas because it it in short supply inrecent days. We appreciate your effort to obtain the gas by yourself.

We recommend not to use sample pins which have copper sleeve, because
turbulence around copper sleeve causes formation of nitrogen ice. When you
use N 2 -cryo, copper sleeve dose not cause any trouble.

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6.

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How to send your dry-shippers1)

You can send your dry-shippers to SPring-8.
------------------------------------------------------------------------------------------SPring-8 Storage Ring Building room D21(between D2 and D3 doors)“Your Name” c.o. BL41XU (or BL45XU)
Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198 JAPAN
Phone: +81-791-58-0802 ext.3362, 3364---------------------------------------------------------------------------------------------2)

Send them to be delivered on weekday, because we cannot receive them onweekend.

3)

Let us know the tracking number, your beamtime and beamline by e-mail(bioxtal@spring8.or.jp).

7.

How to send back your dry-shippers
1)

Prepare the following documents①

Air Waybill(You can get Air Waybill for FedEx at Users Office)②

Commercial invoice③

Other shipping documents(Maybe the same document you need to send it to Japan)

2)Call FedEx to pick up your dry-shippers
When you are asked where FedEx should go to pick up your package,
answer the following address.
--------------------------------------------------------------------------------------------SPring-8/JASRI Users Office (Main building 2F West)
Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198 JAPAN
Phone: +81-791-58-0961

Fax: +81-791-58-0965

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8.

Bring your dry-shippers to the reception counter of Users Office.

Miscellaneous
1)

Attention to your dry-shippers
We recommend you to confirm the soundness of your dry-shippers before
shipping. The defect on the adiabatic vacuum may spoil your samples.

2)

Computer room for users
There is a computer room (D10) outside of the experimental hall. You canuse the room for data processing and data backup.

3)

Account for the beamline PCs
Each research group is provided with his/her own account for the PCs at thebeamlines and the computer room. The initial password is also provided if it
is your first use of the public beamline. You are allowed to change thepassword, but be careful not to forget it. The password of users who does not
use the previous operation period has been initialized.