Product datasheet

Goat Anti-Rabbit IgG H&L (HRP) ab97051
10 Abreviews 1097 References 画像数 7

製品の概要
製品名

Goat Anti-Rabbit IgG H&L (HRP)

由来種

Goat

ターゲット生物種Rabbit

特異性

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and withlight chains common to other Rabbit immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins.

アプリケーション

適用あり : ICC, IHC-P, ELISA, WB

標識

HRP

製品の特性
製品の状態

Liquid

保存方法

Shipped at 4°C. Store at +4°C.

バッファー

pH: 6.80
Constituents: 0.2% BSA, PBS, 0.05% CMIT/MIT based preservative

精製度

Immunogen affinity purified

特記事項（精製）

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads
and conjugated to Horse Radish Peroxidase (HRP).

特記事項（標識）

Molar enzyme/ antibody protein ratio is 4:1

ポリ /モノ

ポリクローナルアイソタイプ

IgG

特記事項

Part of the AbExcel range.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab97051の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

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アプリケーション

特記事項

Abreviews

ICC

Use at an assay dependent concentration.

IHC-P

1/200 - 1/5000.

ELISA

1/10000 - 1/100000.

WB

(10)

1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000;
Chemiluminescent: 1/10000 - 1/50000

画像All lanes : Anti-Estrogen Receptor alpha antibody [E115] - ChIP
Grade (ab32063) at 1/1000 dilution

Lane 1 : Rat pituitary whole tissue lysate
Lane 2 : Mouse pituitary whole tissue lysate
Lysates/proteins at 20 µg per lane.

SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000
dilution
Western blot - Goat Anti-Rabbit IgG H&L (HRP)(ab97051)

Exposure time: 1st lane: 85 seconds
2nd lane: 32 seconds
Blocking and diluting buffer: 5% NFDM/TBST2All lanes : Anti-Estrogen Receptor alpha antibody [E115] - ChIP
Grade (ab32063) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell).
Whole cell lysates

Lane 2 : T-47D (human mammary gland ductal carcinoma
epithelial cell). Whole cell lysates

Lane 3 : MDA-MB231 (Human breast adenocarcinoma epithelialcell) Whole cell lysates (Negative control)

Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell)
Whole cell lysates (Negative control)Western blot - Goat Anti-Rabbit IgG H&L (HRP)

Lane 5 : Human uterus whole tissue lysate(ab97051)

Lane 6 : Human ovary whole tissue lysate
Lane 7 : Human ovary cancer whole tissue lysate
Lysates/proteins at 20 µg per lane.

SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000
dilution

Exposure time: 50 seconds

Blocking and diluting buffer: 5% NFDM/TBST.

Immunohistochemical staining of paraffin embedded humanendometrial carcinoma with purified ab32063 at a working dilutionof 1 in 200. The secondary antibody used is ab97051, a HRP goatanti-rabbit IgG (H+L), at 1/500. The sample is counter-stained withhematoxylin. Antigen retrieval was perfomed using Tris-EDTAbuffer, pH 9.0.

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Goat Anti-Rabbit IgG H&L(HRP) (ab97051)

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Western blot analysis of co-expression Bacillus anthracis
PA83 (A), Pfs48/45 (B) and Pfs48/45-10C with bacterial Endo
H or PNGase F in N. benthamiana plants.
(A) Western blot analysis of co-expression of PA83. Lanes: 1- N.

benthamiana plant was infiltrated with pBI-PA83 construct, for theproduction of glycosylated PA83, 2,3- N. benthamiana plants wereinfiltrated with combinations of the pBI-Endo H/pBI-PA83 or pBIPNGase F/pBI-PA83 constructs, for the production of Endo H (2) orPNGase F (3) deglycosylated PA83 proteins.
(B) Western blot analysis of co-expression of Pfs48/45. Lanes: 1-N.
Western blot - Goat Anti-Rabbit IgG H&L (HRP)(ab97051)
Mamedov et al PLoS One. 2017 Aug
21;12(8):e0183589. doi:
10.1371/journal.pone.0183589. eCollection 2017. Fig 4.
Reproduced under the Creative Commons license
http://creativecommons.org/licenses/by/4.0/

benthamiana plant was infiltrated with pEAQ-Pfs48/45 construct forthe production of glycosylated Pfs48/45;2,3- N. benthamiana plants
were infiltrated with combinations of the pBI-Endo H/pEAQPfs48/45 or pBI-PNGase F/pEAQ-Pfs48/45constructs for theproduction of Endo H (2) and PNGase F (3) deglycosylated
Pfs48/45 proteins.
(C) Western blot analysis of co-expression of Pfs48/45-10C. Lanes:
1- N. benthamiana plant was infiltrated with pEAQ-Pfs48/45-10C
construct for the production of glycosylated Pfs48/45-10C; 2,3- N.

benthamiana plants were infiltrated with combinations of the pBIEndo H/pEAQ-Pfs48/45 or pBI-PNGase F/pEAQPfs48/45constructs for the production of Endo H (2) and PNGase F(3) deglycosylated Pfs48/45-10C proteins. gPA83- glycosylated
PA83; dPA83- deglycosylated PA83; gPfs48/45: glycosylated
Pfs48/45; dPfs48/45: deglycosylated Pfs48/45; gPfs48/45-10C:
glycosylated Pfs48/45-10C; dPfs48/45-10C: deglycosylated
Pfs48/45-10C.
M: MagicMark XP Western Protein Standard. PA83 proteins weredetected using the anti-Bacillus anthracis protective antigen
antibody BAP0101 (Cat. No. ab1988, Abcam); Ps48/45, Endo H orPNGase F proteins were detected using the anti-FLAG antibody.
Pfs48/45-10C protein was detected using the purified anti-His Tagantibody.

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IHC image of beta Actin staining in normal human colon, formalinfixed and paraffin-embedded tissue*.
The section was pre-treated using pressure cooker heat mediatedantigen retrieval with sodium citrate buffer (pH 6) for 30mins. The
section was incubated with ab8227, 3 µg/ml overnight at +4°C. An
HRP-conjugated secondary (ab97051, 1/2000 dilution) was used
for 1hr at room temperature. The section was counterstained withhematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500
dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Goat Anti-Rabbit IgG H&L*Tissue obtained from the Human Research Tissue Bank,
supported by the NIHR Cambridge Biomedical Research Centre(HRP) (ab97051)

Anti-LAMP2 antibody (ab37024) at 1/1000 dilution + Mouse brain
whole tissue lysate at 30 µg

SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Western blot - Goat Anti-Rabbit IgG H&L (HRP)

Exposure time: 1 minute(ab97051)
This image is courtesy of an anonymous Abreview.

10 % gel. Blocked with 5% BSA for 2 hours at 25°C.
Incubated with the primary antibody for 1 hour in TBS-tween at25°C.

5All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell
LysateLysates/proteins at 10 µg per lane.

SecondaryLanes 1-2 : Rabbit polyclonal to GNAT2 (ab97501) at 1/2000
dilution

Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at
Western blot - Goat Anti-Rabbit IgG H&L (HRP)

1/10000 dilution(ab97051)

Lanes 5-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at
1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.

Exposure time: 10 seconds

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