Product datasheet

Human Histone H3 (tri methyl K36) peptide ab1785
1 Abreviews 5 References 画像数 1
製品の詳細
製品名

Human Histone H3 (tri methyl K36) peptide

精製度> 90 % HPLC.

アクセッション番号P68431

Animal freeNo

由来

Synthetic

生物種

Human修飾

tri methyl K37

特性

Our Abpromise guarantee covers the use of ab1785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション

Dot blot
Blocking - Blocking peptide for Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050)

製品の状態

Liquid

備考- First try to dissolve a small amount of peptide in either water or buffer. The more charged
residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water orbuffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does notdissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution iscloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just priorto use.

前処理および保存
保存方法および安定性

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
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Information available upon request.

関連情報機能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting
DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby
play a central role in transcription regulation, DNA repair, DNA replication and chromosomalstability. DNA accessibility is regulated via a complex set of post-translational modifications ofhistones, also called histone code, and nucleosome remodeling.

配列類似性

Belongs to the histone H3 family.

発生段階

Expressed during S phase, then expression strongly decreases as cell division slows down
during the process of differentiation.

翻訳後修飾

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs
methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac)
favors methylation at Arg-18 (H3R17me).
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation andrepresses transcription.
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation.
Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression.
Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and ismutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at
the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while
it is absent on active promoters.
Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to geneactivation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4.
Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses
and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me)are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1
proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11(H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me)
require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) andLys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated
during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome
condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation
at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it
enables the transcription of genes following external stimulation, like mitogens, stress, growth
factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun.
Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation atLys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph)by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) fromheterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory
mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK
isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation
at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation thatprevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specificallyphosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1.
Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional
activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C.
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Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) fromchromatin.
Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)Jrecombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response toultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate
DNA accessibility to repair proteins.
細胞内局在Nucleus. Chromosome.

画像

ab1785 (at 0.01 and 0.1 μg) used in dot blot to test for crossreactivity of ab9050 (1/2500). An HRP-conjugated mouse antirabbit IgG polyclonal (1/10000) was used as the secondary
antibody.

Dot Blot - Human Histone H3 (tri methyl K36)
peptide (ab1785)
This image is courtesy of an anonymous Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"

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