The 23rd Symposium of the Society of Biocatalysis Japan

28th and 29th September, 2023
Inamori Auditorium, Kagoshima University
1-21-40 Korimoto, Kagoshima 890-0065 Japan
Organized by The Society of Biocatalysis Japan
Sponsored byDaicel Corporation
Amano Enzyme Inc.
KNC Laboratories Co., Ltd.
KANEKA CORPORATION
Ajinomoto Co., Ltd.

●Access to Inamori Auditorium

FacadeMain hall

●Wifi connectivity
Wifi connection is available via "eduroam".
Visitor accounts will be created during the symposium. Please ask at the registration
desk if you are necessary.

PROGRAM AT A GLANCE
Thursday, 28th September, 2023
In English Day
9:00- 9:20
9:20- 9:30
9:30-10:30
10:30-10:45
10:45-11:45
13:00-13:40
13:40-14:40
14:40-14:55
14:55-15:55
16:00-16:40
16:40-17:20
18:00-20:00

Registration
Announcement about the schedule of this meeting& Opening Remark
IL1 : Prof. Uwe Bornscheurer (University of Greifswald, Germany)
1 min short presentation for poster (odds number)
Podd & PH : Poster presentaion (odds number & High School Students)
Lunch
IL2 : Dr. Josh K Michener (Oak Ridge National Laboratory, USA)IL3 : Prof. Michael Mueller (University of Freiburg, Germany)
1 min short presentation for poster (even number)
Peven : Poster presentation (even number)
IL4 : Prof. Takeshi Sugai (Keio University, Japan)
O1, O2 : Oral presentation
Banquet (at Study Lounge, Student Community Plaza)

Friday, 29th September, 2023
In Japanese Day
9:00- 9:40
9:40-10:20
10:35-10:55
11:00-12:00
13:15-14:15
14:15-14:55
15:15-15:55
15:55-16:35
16:40-17:00

IL5 : Prof. Masato Ikeda (Gifu University, Japan)
IL6 : Prof. Seiji Negoro (University of Hyogo, Japan)
BreakOH1 : Special oral presentation (Ikeda Gakuen High school)
O3-O5 : Oral presentation
Lunch
O6-O8 : Oral presentation
O9, O10 : Oral presentation
BreakIL7 : Prof. Kohsuke Honda (Osaka University, Japan)
IL8 : Prof. Hiroshi Kanzaki (Okayama University, Japan)
Award Ceremony& Closing Remark● Core Times of Poster Presentation on Thursday, 28th September
10:45-11:45 : odds number & High School Students
14:55-15:55 : even number
Posters should be displayed until 15:00 (break time in afternoon) of the second day.
● Oral Presentation Details
10 min presentation followed by 9 min Q&A (1 min changeover time)LIST OF INVITED LECTURES
IL1Enzyme discovery & engineering to create suitable biocatalysts
Uwe T. Bornscheuer
Institute of Biochemistry, University of Greifswald, Greifswald, Germany.

IL2

Biocatalyst discovery for sustainable chemical production and recycling
Joshua K. Michener
Oak Ridge National Laboratory, USA.

IL3

Green versus Sustainable Chemistry: some insights from biocatalytic and (retro)biosynthetic
analyses
Michael Mueller
Institute of Pharmaceutical Sciences, University of Freiburg, Freiburg, Germany.

IL4

Chemo-enzymatic transformation of carbohydrates and glycosides towards the syntheses ofbioactive substances
Takeshi Sugai
Faculty of Pharmacy, Keio University, Japan.

IL5Modular and hybrid molecular design to construct supramolecular materials
Masato Ikeda
Gifu University, Japan.

IL6

Structural and functional analysis of nylon hydrolase and enzymatic approach for recycling ofnylons
Seiji Negoro, Dai-ichiro Kato and Naoki Shibata
University of Hyogo, Kagoshima University, Japan.

IL7

Design and Implementation of In Vitro Synthetic Pathway with Thermophilic Enzymes
Kohsuke Honda
International Center for Biotechnology, Osaka University, Japan.

IL8

Biocatalyst-mediated synthesis of bioactive ‘Unnatural’ natural products
Hiroshi Kanzaki
Okayama University, Japan

LIST OF ORAL PRESENTATIONSO1Dynamic Kinetic Resolution of 2-Hydroxy Biaryl Atropisomers via Lipase-Catalyzed
Enantioselective O-Acylation○ Neha Dhiman, Gamal A.I. Moustafa, Kengo Kasama, Kyohei Kanomata, Shuji Akai
Graduate School of Pharmaceutical Sciences, Osaka University

O2

Biocatalytic reduction of prochiral bulky-bulky ketones by engineered alcohol dehydrogenase〇Zhongyao Tang1, Yuuki Takagi1, Afifa Ayu Koesoema1, Tomoko Matsuda
Department of Life Science and Technology, School of Life Science and Technology, Tokyo Instituteof Technology

O3(P11)Development of Chemical Pretreatment Techniques for High-Efficiency Enzymatic NylonMonomerization○Yuki Shiraishi1, Risa Yokoyama1, Yoko Furuno1, Tatsuo Kaneko2, Seiji Negoro3, Dai-ichiro Kato1
1:Kagoshima University, 2: Jiangnan University, 3:University of Hyogo

O4

Soluble production of L-amino acid oxidase by mutagenesis towards biotransformation via
keto acid〇Ayuta Nakahara, Wakana Asanuma, Yosuke Toyotake, Mamoru Wakayama, Daisuke Matsui
Ritsumeikan Univ.

O5

Microbial transformation of polymethoxyfavonoids and there antiobesity effect○Koshin Kimoto, Miki Sugiyama, Ryohei Kono, Hirotoshi Utsunomiya, Yoshiharu Okuno
Advanced engineering Faculty, National institute of Technology, Wakayama College, National institute

O6

Novel polyamide 4-degrading enzyme from a marine bacterium○Saito Yusuke, Honda Masayoshi, Furuno Yoko, Kato Dai-ichiro, Abe Hideki, Yamada Miwa
Iwate Univ., Fac. Agric., Iwate Univ., Fac. Eng., Tokyo Univ. Sci., Grad. Sch. Sci., kagoshima Univ.,
CSRS, RIKENS

O7

Soluble expression of polyamide 4-degrading enzyme in recombinant Escherichia coli
〇Shunko Sato, Miwa Yamada
Iwate University Graduate School, Iwate University

O8Improving the ability of lipase-catalyzed dynamic kinetic resolution of tertiary alcohols〇Satoshi Horino, Karla Wagner, Anke Hummel, Kyohei Kanomata, Harald Gröger, Shuji Akai
Graduate school of pharmaceutical sciences Osaka university, Faculty of Chemistry, Bielefelduniversity

O9

A New Family of Peptide Cyclases Enabled Streamlined Chemoenzymatic Synthesis of Cyclic
Peptide〇Kenichi Matsuda, Masakazu Kobayashi, Kei Fujita, Toshiyuki Wakimoto
Faculty of Pharmaceutical Sciences, Hokkaido University

O10(P29)

Lipase/H2SO4 as cocatalyst for dynamic kinetic resolution of alcohols〇Kyohei Kanomata, Jihoon Moon, Takusho Kin, Karin Mizuno, Shuji Akai
Graduate School of Pharmaceutical Sciences, Osaka University

LIST OF POSTER PRESENTATIONS
P01

Synthetic studies of avenolide using biocatalytic reactions as key reactions○Keigo Sugimoto, Keigo Kakumaru, Atsushi Sakurai, Takashi Monna, Ken-ichi Fuhshuku
Department of Interdisciplinary Science and Engineering, Meisei University

P02

Enzymatic Approaches for Enantioselective Lactone Synthesis〇Lan Huong Le Viet, Mayumi Tamura, Tomoko Matsuda
School of Life Science and Technology, Tokyo Institute of Technology

P03

Development of the polyrotaxane accumulated 1-deoxynojirimaycin to control the activity of αglucosidase such as membrane enzyme〇Yuta Tenma, Akihiko Hatano, Atsushi Kato
Shibaura Institute of Technology, University of Toyama

P04

Tannase-catalyzed galloylation of alcohols in the mixed solvent system of hexane/tert-butanol○Renshiro Otaka, Hideo Kojima
Grad. Sch. Sci., Osaka Metropolitan Univ.

P05

Lipase-catalyzed benzyloxycarbonylation of alcohols in the deep eutectic solvent system ofcholine chloride/urea〇Taito Nagamoto, Yuto Yamamoto, Hideo Kojima
Grad. Sch. Sci., Osaka Metropolitan Univ.

P06

Asymmetric reduction of ketones using aquatic plants as whole-cell biocatalyst〇Yuya Machi, Hideo Kojima
Grad. Sch. Sci., Osaka Metropolitan Univ.

P07

Asymmetric reduction of ketones using Pisum sativum seedlings as whole-cell biocatalysts〇Ryota Shimamoto, Yuya Machi, Hideo Kojima, Satomi Takeda
Grad. Sch. Sci., Osaka Metropolitan Univ.

P08

Additive effects on asymmetric reduction of ketones using cyanobacterium Synechocystis sp.
PCC 6803〇Satsuki Fukui, Hideo Kojima, Satomi Takeda, Rio Yamanaka
Grad. Sch. Sci, Osaka Metropolitan Univ.,Pharm. Sci., Himeji Dokkyo Univ.

P09

Lipase-catalyzed allyloxycarbonylation of alcohols using allyl phenyl carbonate〇Yuto Yamamoto, Hideo Kojima
Grad. Sch. Sci., Osaka Metropolitan Univ.

P10

Synthesis of 4-subustituted gentianine from swertiamarin
Sakurako Shimizu, Hinata Matsuo, 〇Ryoya Kaneko, Takaya Noguchi, Yasuaki Hirai, Mikio Fujii
School of Pharmacy, International University of Health and Welfare. The Mt. Fuji Institute for Natureand Biology, Showa University.

P11
(O3)

Development of Chemical Pretreatment Techniques for High-Efficiency Enzymatic Nylon
Monomerization○Yuki Shiraishi1, Risa Yokoyama1, Yoko Furuno1, Tatsuo Kaneko2, Seiji Negoro3, Dai-ichiro Kato1
1:Kagoshima University, 2: Jiangnan University, 3:University of Hyogo

P12

Stereo-selective synthesis of rare alliin isomers using microbial Fe(II)/α-ketoglutaratedependent dioxygenases〇Taku Mizutani, Ryosuke Kanzawa, Ryotaro Hara, Makoto Ueda, Jun Ogawa
Grad., Sch., Agric., Kyoto Univ., NIT, Oyama College

P13

Development of the α-glucosidase inhibitors accumulated 1-deoxynojirimaycinwith DNA origami〇Nagihiro Miyahara, Ryu Mayumi, Akihiko Hatano, Atsushi Kato
Shibaura Institute of Technology, University of Toyama

P14

Characterization and gene cloning of fatty acid amide-synthesizing enzyme in Mycobacterium
sp. AKU 2014
〇Kouhei Yokoo, Tatsuo Fukao, Ryotaro Hara, Makoto Ueda, Jun Ogawa
Grad., Sch., Agric., Kyoto Univ., NIT, Oyama CollegeP15

Enzyme-mediated Enantioselective Hydrolysis of 1,2-Diol Monotosylate Derivatives Bearing a Methallyl Group as a Masked Methyl Ketone Structure○Hiroki Aiba, Yoshihito Takahashi, Yuki Kono, Kazutsugu Matsumoto
Faculty of Science and Engineering, Meisei University

P16

CO2 Fixation Reaction With Thermoplasma acidophilum Malate Dehydrogenase〇Yuri Oku, Kristian Ray Angelo Are, Shusuke Ohshima, and Tomoko Matsuda
Department of Life Science and Technology, Tokyo Institute of Technology

P17

Distribution of mammalian gut microbiota capable of converting C-glycoside mangiferin into its
aglycone norathyriol〇 Y. Uga (M2) 1, U. Hasanah 1, H. Kayaki 2, H. Kawakami 2, Y. Nishitani 2, H. Kuwahara 2,T. Nitoda 1, H. Kanzaki 1
1 Grad. Sch. Environ. Life Sci., Okayama Univ. ， 2 Maruzen Pharmaceuticals

P18

Microbial production of biodegradable polymer by oleaginous yeast Lipomyces starkeyi〇Akihiro Ishioka, Prihardi Kahar, Chiaki Ogino
Faculty of Engineering, Kobe University

P19

Development of nylon hydrolase (nylC) for long-chain nylon degradation
Yukino Teraoka, Chiaki Ogino, Yutaro Mori
Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University

P20

Construction and validation of a metabolic model for thermophilic actinomycetes〇Togo Yamada, Chiaki Ogino, Yutaro Mori
Faculty of Engineering, Kobe University

P21

Modification of nanobody with SpyTag-SpyCatcher to PAA-TiOx NPs〇Tsubasa Matsuzawa, Chiaki Ogino
Faculty of Engineering, Kobe University

P22

Differences between wild type and hyphae-dispersed type of Aspergillus oryzae in theobservation of cell organells〇Suzuki Tomohiro1, Matsumoto Kotone1, Wakai Satoshi2,3, Kondo Akihiko2, Chiaki Ogino
Grad. Sch. Eng, Kobe Univ. Grad. Sch. Sci. Technol. Innov., Kobe Univ. JAMSTEC

P23

Inhibitory sensitivity of β-N-acetylglucosaminidase from Enterococcus sp. MK26 toN,N,N-trimethyl-β-D-glucosaminium-containing compounds〇K. Morioka, H. Ono, M. Koide, S. Suganuma, T. Nitoda, H. Kanzaki
Grad. Sch. Environ. Life Sci., Okayama Univ.

P24

Development of L-penicillamine production process by enzymatic reaction◯Toru Watanabe, Yuichi Iwamoto, Akira Iwasaki.
KANEKA CORPORATION

P25

Discovery of a giant enzyme for plant secondary metabolism: Purification and characterizationof tuliposide B-converting enzyme in a wild tulip
Taiji NOMURA, ○Yasuo KATO
Biotechnol. Res. Cent., Toyama Pref. Univ.

P26

Laccase-catalyzed synthesis of an odor-active compound, rotundone, and purification○Satoru Umezawa, Yuki Tokura, Kuniki Kino
T. Hasegawa Co., LTD., . Dept. Appl. Chem., Sch. Adv. Sci. Eng., Waseda Univ.

P27

The effects of amino acid substitution on the nylon hydrolytic activity of NylC-like proteinfrom Leucobacter triazinivorans (LtNylC)◯Sayaka Iizasa, Dai-ichiro KatoGraduate School of Science and Engineering, Kagoshima University

P28

A novel marine bacterium degrading the monomer unit of itaconate-derived nylon-6i〇Yoko Furuno1, Risa Yokoyama1, Kaito Miyazaki1, Tatsuo Kaneko2, Seiji Negoro3,Dai-ichiro Kato1
1: Kagoshima University, 2: Jiangnan University, 3: University of Hyogo

P29(O10)

Lipase/H2SO4 as cocatalyst for dynamic kinetic resolution of alcohols〇Kyohei Kanomata, Jihoon Moon, Takusho Kin, Karin Mizuno, Shuji Akai
Graduate School of Pharmaceutical Sciences, Osaka University

LIST OF ORAL & POSTER PRESENTIONSBY HIGH SCHOOL STUDENTS
PH01
OH1Volcanic gases measurement in Sakurajima volcanic area using simple alkaline filter paper
method○Yu Yoshii, ○Chiyono Kawamoto, ○Kokoro Kurose
Ikeda High School

PH02

Study on the transmitted light of cellophane〇Momoka Iwakiri, 〇Daichi Uehara, 〇Ryutaro Uchiyama, Ryoga Mizuguchi, Taichi Yuasa
Ikeda high school

PH03

Stress Experienced by Zebra Fish in Saltwater
Ryutaro Okamura, 〇Yuta Ito, 〇Yuimi Ryuzozi, Kanta Suzuki, Taketo Uehigashi
Ikeda Gakuen Ikeda Junior & Senior High School

PH04

Cherry Blossom Forecasting - Proposal of Ikeda Model
Rin Amiya, Masato Ina, Yuu Hamasaki, Kayo Nakazono, Himawari Iwai, Yori Sonoda
Ikeda High School

PH05

Analysis of visible color differences
Tatsuya Uemoto, Kaiji Sakamoto, Hirokazu Tokunaga, Reia Watanabe
Ikeda High school

PH06

Development of a measuring device for phosphorus concentration in rivers and sea〇Yumeno IJICHI,〇Aoi MIZUMOTO,Yuki SAKODA,Keisuke MATSUOKA,Saya ISHIZAKI
Kokubu High School

PH07

Distribution of the Hepaticae Leptolejeunea elliptica in Kirishima Geopark, Kagoshima○Riko Yaeo, ○Natsune Yamamoto, Honoka Sako and Miu YotsumotoKokubu High School

PH08

First records of the freshwater shrimp Caridina laoagensis (Decapoda: Atyidae) in the Kyushu
Island, Yakushima Island and Kuchinoerabujima Island, Japan○Kantaro Souma, Kousei Sumiyoshi and Koumei Tamura
Kokubu High School

PH09

Recent distribution and egg bearing status of freshwater caridean shrimps and prawns(Atyidae and Palaemonidae) in Kagoshima Prefecture, Japan○Shuto Kawano, Takahiro Tsuzaki and Masahiko Machida
Kokubu High School