Product datasheet

Human IgE protein (Azide free) ab65866
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製品の詳細
製品名

Human IgE protein (Azide free)

生理活性1 mg of ab65866 is 380,000± 50,000 IU/ml.

精製度> 90 % SDS-PAGE.
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from amonoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining
contaminants are mainly components from Foetal Bovine Serum.

エンドトキシン・レベル< 25.000 Eu/mg

発現系

Monoclonal Hybridoma

アクセッション番号P0DOX4P0DOX7タンパク質長Full length protein

Animal freeNo

由来

Nativeアミノ酸配列 1
生物種

Human

配列の追加情報

Observed band of ~185kDa on SDS-PAGE

アミノ酸配列 2
生物種

Human

特性

Our Abpromise guarantee covers the use of ab65866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション

SDS-PAGE
IHC - WholemountWestern blot
ELISA
Immunocytochemistry

製品の状態

Liquid1

備考This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal
hybridoma.
This antigen is quite unique, because it is the only available IgE product, which originates from ahuman monoclonal cell line. Our IgE product shows superior advantage when compared to thetraditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior
on the following key parameters:.
- Low batch-to-batch variation- Higher degree of purity (>90%)- A more uniform product (monoclonal and not polyclonal)
Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cellproliferation studies.

前処理および保存
保存方法および安定性

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C longterm. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.00017% PBS, 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報
関連性

IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect
against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgEinteracts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and othersubstances that lead to the itching, sneezing and congestion of allergies - and the life threatening
respiratory distress of asthma and anaphylactic shock.

細胞内局在

Cell Membrane

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Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Native Human IgE protein (Azidefree) (ab65866)

SDS-PAGE analysis of:Lane 1: Molecular weight markers.
Lane 2: ab65866.
Lane 3: IgE purified from serum from a myeloma patient.

SDS-PAGE - Native Human IgE protein (Azide free)(ab65866)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"

Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,please visit https://www.abcam.co.jp/abpromise or contact our technical team.

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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors

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