Quality Results for life

PRODUCT CATALOGUE

SepaSperm®
〇 For sperm preparationby the density gradient centrifugation.
〇 Free from animal derivatives.
〇 SepaSperm® Wash Solution is HEPES buffered.

SepaSperm® Solution 100%
SepaSperm® Solution 80%
SepaSperm® Solution 60%
SepaSperm® Solution 40%
SepaSperm® Wash Solution

REF

CodeContents

92143

SE100G-100

100mL

92073

SE80G-100

100mL

92079

SE80G-50

50mL

92074

SE60G-100

100mL

92080

SE60G-50

50mL

92075

SE40G-100

100ml

92081

SE40G-50

50ml

92147

SEWSG-100100ml

COMPONENTSQUALITY CONTROLCalcium chloride / Gentamicin sulfate / Glucose / HEPES /pH 7.2-7.6 / Osmolarity 270-300 mOsm/L / Endotoxin <0.25EU/mL /Mouse Embryo Assay ≥80% / Sperm Survival（24h）≥80% /Magnesium sulfate / Potassium chloride / Potassium phosphate /Recombinant human albumin / Silica particles / Sodium bicarbonate /Sperm Penetration ≥3 / Sterility Test

Sodium chloride / Sodium lactate / Sodium pyruvate

Storage: 2-8℃
Shelf life: 18 months

RESULT
Results of SepaSperm®
Comparison of progressive motility rates
after density gradient centrifugation(%)
10094.2%80(%)
10093.4%

80

6040

Comparison of ferilization rates
after C-IVF

60
53.9%

51.1%

2071.0%75.6%

Product ASepaSperm®

4014.4%

9.7%

2000Before■ Product A

After■ SepaSperm®

Progressive
motility rates

NBeforeAfter

Progressive
motility rates

Sperm Concentration

Reference: Shinjuku ART Clinic（×10⁶/ml）

Sperm motility rate（%）

Sperm motility
recovery rate（%）

Semen

5

128.7±40.0

58.7±19.7

ーPrepared with SepaSperm® Solution

5

126.5±50.4

91.5±6.5

26.6±7.8

Prepared with Product A5114.7±55.1

90.3±7.922.4±5.5

Numbers in mean±SD

Reference: Kusuhara Women’s Clinic
Specification may change without pre-notice for purpose of product improvement.

Kitazato Corporation
HEADQUATER : 100-10 Yanagishima, Fuji, Shizuoka 416-0932 JAPANTOKYO : 1-1-8 Shibadaimon, Minato-ku, Tokyo 105-0012 JAPAN
Mail

contact@kitazato.co.jp
Copyright© Kitazato Corporation All Rights Reserved. | v.1.0 2023.12.14

A n e xa m p l e of s i n g l e - l a y e r g ra d i e nt c e nt r i f u g a t i o n1Bring all solutions to room temperature（20-25°C）before use. Prepare two 15mL centrifuge tubes.
One of them with 3-5mL of SepaSperm® 80%. Ⓐ The other with 5mL of SepaSperm® Wash Solution. Ⓑ

2

Check the semen volume with a 5mL pipette or similar and evaluate the semen sample with a sperm count chamber.

3

Pipette semen on top of the prepared SepaSperm® 80%. Stir 2cm vertically around the layers between the semen and SepaSperm® 80%

4Centrifuge for 15 minutes at approximately 600 x g.（Separation of sperm and seminal fluid）
Depending on the state of collected sperm, adjust xg or centrifugation time accordingly.

5After centrifugation, remove the supernatant with a new pipette.

6Add 5mL of SepaSperm® Wash Solution（SEWS）to the sperm pellet and resuspend. Centrifuge at 400 x g for 5 minutes for washing.
Depending on the state of collected sperm, adjust xg or centrifugation time accordingly.

7

Remove the supernatant leaving the sperm pellet only. Dilute the sperm pellet with SepaSperm® Wash Solution according to thefollowing treatment.

SepaSperm®
80%
3-5mLⒶ

Stir vertically

SepaSperm®
Wash Solution
5ｍLⒷ

New pipettesⒶTo a new tube Ⓑ

Supernatant

SepaSperm®
Wash Solution
5ｍL

600G
15 minSperm pellet

400G
5 min
Sperm pellet

A n e xa m p l e of t w o - l a y e r de n s i t y g ra d i e nt c e nt r i f u g a t i o n1Bring all the solutions to room temperature（20-25°C）before use. Prepare two 15mL centrifuge tubes.
One of them with layers in the next order from bottom to up ;❶ SepaSperm® 80% 1.5-2.0mL ❷ SepaSperm® 40% 1.5 -2.0mL ❸ Liquified semen
The other with 5mL of SepaSperm® Wash Solution.

2Check the semen volume with a 5mL pipette or similar and evaluate the semen sample with a sperm count chamber.

3Centrifuge the tube at 600 x g for 15 minutes.（Separation of sperm and seminal fluid）
Depending on the state of collected sperm, adjust xg or centrifugation time accordingly.

4

Aspirate the sperm pellet.

5Transfer the sperm pellet to a new centrifuge tube prepared with 5mL of SepaSperm® Wash Solution, and mix well.

6Centrifuge at 400 x g for 5 minutes for washing.
Depending on the state of collected sperm, adjust xg or centrifugation time accordingly.

7Remove the supernatant leaving the sperm pellet only. Dilute the sperm pellet with SepaSperm® Wash Solution according to thefollowing treatment.

Syringe❸ liquefied semen❷ SepaSperm® 40%
1.5mL ～ 2.0mL❶ SepaSperm® 80%

1.5mL ～ 2.0mLⒶSepaSperm®
Wash Solution5ｍLⒷ

New pipette

Transfer
Cannula
600G
15 minⒶ

To a new tube

Ⓑ

40%
80%

Sperm pellet