Product datasheet

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113
15 Abreviews 1264 References 画像数 8

製品の概要
製品名

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

由来種

Goat

ターゲット生物種

Mouse

アプリケーション

適用あり : IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-P

免疫原

Other Immunogen Type corresponding to Mouse IgG.

標識

Alexa Fluor® 488. Ex: 495nm, Em: 519nm

製品の特性
製品の状態

Liquid

保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

バッファー

Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA

精製度

Immunogen affinity purified

特記事項（精製）

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

ポリ /モノ

ポリクローナルアイソタイプ

IgG

特記事項

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher ScientificCompany. The Alexa Fluor® dye included in this product is provided under an intellectual property
license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, thepurchase of this product conveys to the buyer the non-transferable right to use the purchased
product and components of the product only in research conducted by the buyer (whether thebuyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale ofthis product is expressly conditioned on the buyer not using the product or its components, or any
materials made using the product or its components, in any activity to generate revenue, which
may include, but is not limited to use of the product or its components: in manufacturing; (ii) toprovide a service, information, or data in return for payment (iii) for therapeutic, diagnostic orprophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research.
For information on purchasing a license to this product for purposes other than research, contact
Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or1

outlicensing@thermofisher.com.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab150113の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション

特記事項

Abreviews

IHC-Fr(3)Use at an assay dependent concentration.

ICC/IF(6)

1/200 - 1/1000.

ELISA

Use at an assay dependent concentration.

Flow Cyt

1/2000.

ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), issuitable for use as an isotype control to complement thissecondary antibody.

IHC-P

(3)

Use at an assay dependent concentration.

画像

ICC/IF image of ab7291 stained HeLa cells. The cells were 100%
methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5
minutes and then incubated in 1% BSA / 10% normal goat serum /0.3M glycine in 0.1% PBS-Tween for 1h to block non-specificprotein-protein interactions. The cells were then incubated with theantibody (ab7291, 1µg/ml) overnight at +4°C. The secondaryantibody (green) was ab150113 Alexa Fluor® 488 goat anti-mouse
IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cellnuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay todemonstrate low non-specific binding of the secondary antibody.
Immunocytochemistry/ Immunofluorescence - GoatAnti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

2Overlay histogram showing HeLa cells stained with ab29 (red line).
The cells were fixed with 80% methanol (5 min) and thenpermeabilized with 0.1% PBS-Tween 20 for 20 min. The cells werethen incubated in 1x PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein interactions followed by theantibody (ab29, 0.1µg/1x106 cells) for 30 min at 22°C. The
secondary antibody used was Alexa Fluor® 488 goat anti-mouseIgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotypecontrol antibody (black line) was mouse IgG2a [ICIGG2A](ab91361, 0.1µg/1x106 cells) used under the same conditions.
Unlabelled sample (blue line) was also used as a control.
Flow Cytometry - Goat Anti-Mouse IgG H&L (AlexaAcquisition of >5,000 events were collected using a 20mW ArgonFluor® 488) (ab150113)

ion laser (488nm) and 525/30 bandpass filter.

Cross-reactivity of the polyclonal secondary antibody ab182017
was tested using a sandwich ELISA approach. The wells werecoated with the indicated IgG standards at 1 µg/ml (50µl/well) andincubated overnight at 4°C, followed by a 5% BSA blocking step for
2h at RT. ab182017 was then added starting at 1 µg/ml andgradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For
the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used
at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

For the batch tested, ab182017 showed a cross-reactivity
below 2% towards Chicken IgY, 6% towards Human IgG, 7%towards Rabbit IgG and 47% towards Rat IgG.
ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor®
488) (ab150113)

These data were developed using the unconjugated antibody(ab182017).

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Cross-reactivity of Goat anti-Mouse IgG H&L (ab182017) and Goatanti-Mouse IgG H&L obtained from two different vendors was tested
using a sandwich ELISA approach. The wells were coated with theindicated IgG standards (Rabbit, Human, Mouse and Rat) at 1µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5%BSA blocking step for 2h at RT. Secondary antibodies were thenadded starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well),followed by incubation for 2h. For the detection Donkey anti-GoatIgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well),followed by incubation for 1h at RT. This data is from a
representative dilution.
ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor®

These data were developed using the unconjugated antibody488) (ab150113)(ab182017).

Overlay histogram showing SV40LT-SMC cells stained with

ab7817 (red line). The cells were fixed with 4% formaldehyde (10min) and then permeabilized with 0.1% PBS-Triton X-100 for 15min. The cells were then incubated in 1x PBS / 10% normal goat
serum to block non-specific protein-protein interactions followed by
the antibody (ab7817, 0.1µg/1x106 cells) for 30 min at 22°C. The
secondary antibody used was Goat Anti-Mouse IgG H&L (AlexaFluor® 488) (ab150113) at 1/2000 dilution for 30 min at 22°C
Isotype control antibody (black line) was mouse IgG2a[18C8BC7AD10] (ab170191) used at the same concentration andconditions as the primary antibody. Unlabelled sample (blue line)
Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexawas also used as a control.

Fluor® 488) (ab150113)

Acquisition of >5,000 events were collected using a 50 mW Bluelaser (488nm) and 530/30 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 80%
methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15
min used under the same conditions.

4Overlay histogram showing HeLa cells stained with ab26 (red line).
The cells were fixed with 80% methanol (5 min) and thenpermeabilized with 0.1% PBS-Tween for 20 min. The cells werethen incubated in 1x PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein interactions followed by theantibody (ab26, 1µg/1x10^6 cells) for 30 min at 22ºC. The
secondary antibody used was Alexa Fluor® 488 goat anti-mouseIgG (H&L) at 1/2000 dilution for 30 min at 22ºC. Isotype control
Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexaantibody (black line) was mouse IgG1 [ICIGG1] (ab91353,Fluor® 488) (ab150113)

2µg/1x10^6 cells) used under the same conditions. Unlabelledsample (blue line) was also used as a control. Acquisition of >5,000
events were collected using a 20mW Argon ion laser (488nm) and
525/30 bandpass filter. This antibody gave a positive signal in
HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilizedwith 0.1% PBS-Tween for 20 min used under the same conditions.

IHC - Wholemount of Caenorhabditis elegans embryo labelling RNA
polymerase II CTD repeat YSPTSPS with ab817. Sample wasincubated with primary antibody (1/100 in PBS + 3% BSA + 0.1%
Triton-X 100) for 24 hours at 4°C. ab150113, an Alexa Fluor® 488conjugated goat anti-mouse IgG polyclonal (undiluted) was used as
the secondary antibody.
IHC - Wholemount - Goat Anti-Mouse IgG H&L(Alexa Fluor® 488) (ab150113)
This image is courtesy of an anonymous Abreview.

Alexa Fluor® - Goat Anti-Mouse IgG H&L (AlexaFluor® 488) (ab150113)

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