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100bp Ladder Marker
First issue : Nov. 20, 2008

No. ００１５０Ｆ
MSDS MSDS
No.

Material Safety Data Sheet

1 PRODUCT AND COMPANY IDENTIFICATION
Product Name
Product CodeSUPPLIERName
Address
Emergency Telephone No.
Fax No.

100bp Ladder Marker
DNA-035
Biotech support Department TOYOBO Co., Ltd.
2-2-8 Dojima Hama Kita-ku Osaka, 530-8230 Japan+81-6-6348-3786+81-6-6348-3833

2 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical Product(Substance/Preparation)
Chemical NatureParts Name
100bp Ladder Marker

6x Loading Dye

Preparation

Main components
PolynucleotideTris(Hydroxymethyl)aminomethane
EDTA・2NaTris(Hydroxymethyl)aminomethane
EDTA・2Na
GlycerolBromophenol blue
Orange G

CAS No.
77-86-1
6381-92-6
77-86-1
6381-92-6
56-81-5
115-39-9
1936-15-8

Components Contributing to the Hazard
Common Chemical Name
Glycerol(or Generic Name)
Synonyms
Glycerin
Chemical formula
CH2OHCHOHCH2OH

56-81-5
6x Loading Dye: 30%

CAS No.
Concentration

3 HAZARDS IDENTIFICATION
Most Important Hazards
Specific Hazards

May cause eye and skin irritation. May cause respiratory anddigestive tract irritation.
Nothing paticular

4 FIRST-AID MEASURES
Inhalation

Skin Contact
Eye Contact
Ingestion

In case of irritation by inhaling this product, move affected person tofresh air and await recovery. If irritation persists, seek immediate
medical attention.
Wash with clean water, immediately.
Flush eyes with clean water for more than 15 min, and seek medical
attention.
Induce vomiting if possible, seek medical attention.

5 FIRE-FIGHTING MEASURESExtinguishing Media
Protection of fire-fighters

Water, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side of fire area.
Fire-fighters should wear proper protective equipment in case of large
scale fire.

2/3100bp Ladder Marker
First issue : Nov. 20, 2008

No. ００１５０Ｆ
MSDS MSDS
No.

Material Safety Data Sheet

6 ACCIDENTAL RELEASE MEASURES

Personal Precautions
Avoid substance contact and inhalation.
Environmental Precautions Avoid disposition to the environment.
Methods for Cleaning up
Wipe up with a disposable wipe, place in a bag and hold for waste

7 HANDLING AND STORAGE
HANDLING
Technical Measures
Precautions
Safe Handling Advice
STORAGETechnical Measures
Storage Conditions
Packaging Materials

Pay attention to contact to the human body.
Avoid substance contact. After handling, wash with clean water.
Good laboratory technique should be used when handling this product.

Store in the laboratory bottle.
Store at -20℃.
Store in the original package.

8 EXPOSURE CONTROLS/ PERSONAL PROTECTION
ENGINEERING MEASURES Set up good ventilation and exhaust system in the work area.

Control Parameter
Limit Values
Glycerol
JSOH OEL
Not established3
ACGIH TLV
10mg/mOSHA PEL
Not establishedPERSONAL PROTECTIVE EQUIPMENT
Wear a dust mask as appropriate.
Respiratory ProtectionHand Protection
Chemical safety gloves
Eye Protection
Chemical safety goggles
Skin and Body Protection Long sleeves to prevent contact with skin.

9 PHYSICAL AND CHEMICAL PROPERTIES
Physical StateColourOdour
pH
Flash Point
Explosion limit
Density
Solubility

Liquid at room temperature, solid at -20℃
100bp Ladder Marker: colorless
6x Loading Dye: blue solution
None
7.0-8.0
No inflammbility due to solution, but glycerol whose flash point is160℃ may stay behind after volatilization(6x Loading Dye).
Not explosible
Not available
Soluble in water

10 STABILITY AND REACTIVITY
Stability

Stable at -20℃

Possible Hazardous Reactions NoneConditions to Avoid
Material to Avoid
Hazardous Decomposition
ProductHigh Temperature, direct sunlight
Strong oxidizers and strong reducers
Not available

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100bp Ladder Marker
First issue : Nov. 20, 2008

No. ００１５０Ｆ
MSDS MSDS
No.

Material Safety Data Sheet

11 TOXICOLOGICAL INFORMATION
Acute Toxicity
Local Effects

Not available
May cause eye and skin irritation. May cause respiratory anddigestive tract irritation.

12 ECOLOGICAL INFORMATIONMobility
Persistence/Degradability
Bioaccumulation

Soluble in water and diffusible into water environment.
Not available
Not available

13 DISPOSAL CONSIDERATIONS
Waste from Residues
Contaminated Packaging

Dispose of in accordance with all applicable local and national lawsand regulations.
Dispose in the same way as the material.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification Number
Specific Precautions

Not classified
Follow all of the laws and regulations in your respective country.
To prevent packages from breaking, handle with care.

15 REGULATORY INFORMATION
Follow all of the laws and regulations in your country.

16 OTHER INFORMATION
Notice

The information in this MSDS, to the best of our knowledge, isaccurate and correct. However, TOYOBO makes no warranty andassumes no liability whatsoever in connection with any use of thisinformation. The MSDS is subject to revision as new information
becomes available.