1/6

QuantAccuracyTM, RT-RamDATM cDNA Synthesis Kit
Code：RMQ-101
First issue：Mar. 8th, 2021
Revised : Nov. 23, 2021
SDS No. 1969F

Safety Data Sheet
1 CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
Identification of the productProduct Code
SUPPLIER
Name
Address
Department
Emergency Telephone No.
Fax No.
Recommended use andrestrictions on use

QuantAccuracy

TM, RT-RamDA

TM

cDNA Synthesis Kit

RMQ-101TOYOBO Co., Ltd.
2-8 Dojima Hama 2-chome, Kita-Ku OSAKA 530-8230 JAPAN

Biotechnology Overseas Sales and Marketing+81-6-6348-3843+81-6-6348-3833
cDNA amplification kit for qPCR (Reagents for research)

2 HAZARDS IDENTIFICATION
Most Important Hazards

gDNA Remover, RNase Inhibitor RT-RamDATM Enzyme Mix, and Lysis
Enhancer contain glycerol and may cause eye and skin irritation. Lysis
Buffer contains detergent and may cause eye irritation.

Specific Hazard
Not available
GHS Classification
Hazard class and category

Physical Hazards
Health Hazards

Flammableliquids
Skin corrosion/Irritation
Serious eye damage/Eye irritation

Environmental Hazards

gDNA Remover

RNase Inhibitor

Not classified

Not classified

Lysis Buffer,
TM
RT-RamDA
Buffer,
RT-RamDATM
Primer,Nuclease-freewater
Not classified

Category 3

Category 3

Not classified

Category 2B

Category 2B

Not classified

Classification
not possible

Classification
not possible

Classification
not possible

TM

Physical Hazards
Health Hazards

Flammableliquids
Skin corrosion/Irritation
Serious eye damage/Eye irritation

Environmental Hazards
Label elements

Pictogram or symbol
Signal word
Hazard statement

RT-RamDA
Enzyme Mix

Lysis Enhancer

Not classified

Not classified

Category 3

Category 2

Category 2B

Category 2

Classification
not possible

Not classified

RNase Inhibitor
gDNA RemoverTM
RT-RamDA Enzyme Mix
No symbol
Warning
Causes skin irritation
Causes serious eye irritation

Precautionary statement
Prevention
Wash hands thoroughly after handling.

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QuantAccuracyTM, RT-RamDATM cDNA Synthesis Kit
Code：RMQ-101
First issue：Mar. 8th, 2021
Revised : Nov. 23, 2021
SDS No. 1969F

Safety Data Sheet
2 HAZARDS IDENTIFICATION (CONTINUED)
Response

If skin irritation occurs: Get medical advice/attention.
IF IN EYES: Rinse cautiously with water for several minutes. Remove
contact lenses, if present and easy to do. Continue rinsing.
If eye irritation persists: Get medical advice/attention.

Label elements
Pictograms or symbols

Lysis Enhancer

Signal words
Hazard statements

Warning
Causes skin irritation
Causes serious eye irritation
Wash hands thoroughly after handling
Wear protective gloves.
Wear eye protection/face protection.
IF ON SKIN: Wash with plenty of water
IF IN EYES: Rinse cautiously with water for several minutes. Remove
contact lenses, if present and easy to do. Continue rinsing.
If skin irritation occurs: Get medical advice/attention.

Prevention
Response

3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical Product(Substance/Mixture)
Chemical Nature
Parts Name
①Lysis Buffer②Lysis Enhancer③RNase Inhibitor

TM④RT-RamDA

Buffer⑤gDNA Remover⑥RT-RamDATM Enzyme

TM⑦RT-RamDA

Primer

⑧Nuclease free water

MixtureAqueous solution
Main Component
Protease
Surfactant A
Guanidine Thiocyanate
GlycerolDithiothreitol
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
RNase Inhibitor
Tris(hydroxymethyl)aminomethane
Magnesium chloride2'-deoxyadenosine 5'-(tetrahydrogen triphosphate)2'-deoxycytidine 5'-(tetrahydrogen triphosphate)2'-deoxyguanosine 5'-(tetrahydrogen triphosphate)2'-deoxythymidine 5'-(tetrahydrogen triphosphate)
GlycerolTris(hydroxymethyl)aminomethane
Deoxyribonuclease
Tris(hydroxymethyl)aminomethane
Poly(oxyethylene)nonylphenyl ether
GlycerolReverse Transcriptase
RNase Inhibitor
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
Tris(hydroxymethyl)aminomethane
Ethylenediaminetetraacetic aciddisodium salt
Deoxyribonucleic acid
Water

CAS No.
593-84-0
56-81-53483-12-3
7365-45-9
77-86-1
7786-30-3
1927-31-7
102783-51-7
93919-41-6
18423-43-3
56-81-5
77-86-19003-98-9
77-86-1
9016-45-9
56-81-57365-45-9
77-86-1
139-33-3
7732-18-5

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QuantAccuracyTM, RT-RamDATM cDNA Synthesis Kit
Code：RMQ-101
First issue：Mar. 8th, 2021
Revised : Nov. 23, 2021
SDS No. 1969F

Safety Data Sheet
3 COMPOSITION / INFORMATION ON INGREDIENTS(CONTINUED)
Components Contributing to the Hazard
Common Chemical Name
Surfactant A(or Generic Name)
Synonyms-Chemical formula-

CAS No.
Concentration
Common Chemical Name(or Generic Name)
Synonyms
Chemical formula
CAS No.
Concentration

4 FIRST-AID MEASURESInhalation

Skin Contact
Eye Contact

Ingestion

Glycerol

Guanidine
Thiocyanate

Glyceringuanidinium
isothiocyanate

CH2(OH)CH(OH)CH2(OH)56-81-5< 1%approximately50%
4-(2-hydroxyethyl)- Poly(oxyethylene1-piperazineethane)nonylphenyl
sulfonic acidether
HEPES
Nonidet P-40
C8H18N2O4S
Not specified
7365-45-9
9016-45-9< 0.5%< 0.00005%

C2H6N4S
593-84-0< 3%

In case of irritation by inhaling this product, move affected person tofresh air and await recovery. If irritation persists, seek immediate
medical attention.
Wash with plenty of clean water, immediately. If skin irritation occurs:
Get medical advice/attention.
Rinse cautiously with water for several minutes. Remove contact lenses
if present and easy to do. Continue rinsing. If eye irritation persists: Get
medical advice/attention.
Try to get the affected person to vomit as much as possible. Seek medical
attention, immediately.

5 FIRE-FIGHTING MEASURESExtinguishing MediaSpecific extinguishingmethods
Protection of fire-fighters

Water, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side of fire area.
Fire-fighters should wear proper protective equipment in case of large
scale fire.

6 ACCIDENTAL RELEASE MEASURES
Personal Precautions
Environmental Precautions
Methods for Cleaning up

Wear protective equipments and avoid eye/skin contact and inhalation.
Avoid disposition to the environment.
Use cloth, paper, or anything similar to soak up to the solution leaking
out of the container. Take up under vacuum using dust collecting filter.

7 HANDLING AND STORAGE
HANDLING
Technical Measures

Wear protective equipments and avoid contact with eyes and skin.
Handle with ventilation and local exhaust system.
Avoid substance contact.
Keep the handling area always clean. After handling, wash hands

Precautions
Hygiene measures
STORAGE
Proper Storage Conditions Keep tightly closed and store at below -20℃
Packaging Materials
Store in the original package

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QuantAccuracyTM, RT-RamDATM cDNA Synthesis Kit
Code：RMQ-101
First issue：Mar. 8th, 2021
Revised : Nov. 23, 2021
SDS No. 1969F

Safety Data Sheet
8 EXPOSURE CONTROLS/ PERSONAL PROTECTION
Control Parameter
Limit Values

Surfactant A

JSOH OELACGIH TLV

not establishednot established

OSHA PEL

not established

Limit Values

Glycerol(mist)

Guanidine
Thiocyanate

not established
10mg/m3
total dust:
315mg/m TWA
respirable fr.:
35mg/m TWA

not established
not established
not established4-(2-hydroxyethyl)- Poly(oxyethylene1-piperazineethane)nonylphenyl
sulfonic acidether

JSOH OEL
not established
not established
ACGIH TLV
not established
not established
OSHA PEL
not established
not establishedPERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection
Wear a dust mask when needed.
Hand Protection
Wear chemical safety gloves.
Eye ProtectionWear protective eyeglasses or chemical safety goggles.
Skin and Body Protection Wear lab coat when needed.

9 PHYSICAL AND CHEMICAL PROPERTIES

TMPhysical State

Liquid at normal temperature. Lysis Buffer, RT-RamDA
and Nuclease-free Water freeze at -20℃.

ColourOdour
pH
Flash Point

Colourless
Odourless
6.0-9.0
Not flammable due to aqueous solution, but glycerol whose flash point
160°C may stay behind after volatilization.
Not explosive
1.0～1.2(g/cm3)
Soluble in water

Explosion limit
Density
Solubility

10 STABILITY AND REACTIVITY
Stability
Possible Hazardous
ReactionsConditions to Avoid
Material to Avoid
Hazardous Decomposition
ProductStable at below -20℃.
Nothing particularHigh temperature, ignition sources, direct sunlight
Strong oxidizer, strong reducers
Not available

Primer Mix,

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QuantAccuracyTM, RT-RamDATM cDNA Synthesis Kit
Code：RMQ-101
First issue：Mar. 8th, 2021
Revised : Nov. 23, 2021
SDS No. 1969F

Safety Data Sheet
11 TOXICOLOGICAL INFORMATION

Acute Toxicity
Not Available
Local Effects
May cause eye and skin irritation.
Toxicological information on the component of this productSurfactant A
GlycerolSkin corrosion/Irritation
Serious eye damage/Eyeirritation-Rabbit,500mg/24hr:
Mild
Rabbit, undilluted, Rabbit,
OECD405:
500mg/24hr:
Category 1
Mild

12 ECOLOGICAL INFORMATION
Ecotoxicity

Risk ofblindness.
Causes severeburns.

Guanidine
Thiocyanate
Rainboutrout:
Rainboutrout:
Guppy:
LC50(96hr)
LC50(96hr)
C50(96hr)＞0.5 mg/L
54g/L
89.1g/LGold fish:
Daphnia
LC50(24hr)＞magna50(48hr)
5g/L＞42.4g/L
Daphnia magna Green algae:: EC50(24hr)＞ EC50(72hr)
10g/L
130g/L
Redalgae:
Persistence and degradability Glycerol and enzyme or the like is Biodegradable. Surfactant A is not
Bioaccumulative potential
Mobility in soil

Surfactant A

Guanidine
Thiocyanate
Causes burns

Glycerolreadily biodegradable.
Thought to be not bioaccumulative.
Soluble in water and diffusible into water environment.

13 DISPOSAL CONSIDERATIONS
Waste from Residues

Contaminated Packaging

Dispose of in accordance with all applicable local and national laws andregulations.
Dispose of in accordance with all applicable local and national laws andregulations.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification NumberSpecific Precautions

Not classified
Follow all of the laws and regulations in your respective country.
To prevent packages from breaking, handle with care.

15 REGULATORY INFORMATION

Follow all of the laws and regulations in your country.

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QuantAccuracyTM, RT-RamDATM cDNA Synthesis Kit
Code：RMQ-101
First issue：Mar. 8th, 2021
Revised : Nov. 23, 2021
SDS No. 1969F

Safety Data Sheet
16 OTHER INFORMATION
NoticeThis product is sold for research purposes only. It is not intended for food,drug, household, agricultural or cosmetic use. Its use must be supervised
by a technically qualified individual experienced in handling potentiallyhazardous chemicals. The information in this SDS, to the best of ourknowledge, is accurate and correct. However, TOYOBO makes nowarranty and assumes no liability whatsoever in connection with any useof this information. The information shall not be taken as being allinclusive and is to be used only a guide. All materials and mixtures may
be present unknown hazards and should be used with caution. The SDS
is subject to revision as new information becomes available.