1/5rTth DNA polymerase
Oct. 26, 2017

SDS No.1051F

Safety Data Sheet

1 CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
Product Name
Product Code
SUPPLIERName
Address
Emergency Telephone No.

Fax No.
Recommended use andrestrictions on use

rTth DNA polymerase
TTH-301, TTH-3R, TTH-3D, TTH-309, TTH-309L, TTH-329, TTH-329

Biotech support Department TOYOBO Co., Ltd.
2-2-8 Dojima Hama Kita-ku Osaka, 530-8230 Japan+81-6-6348-3786+81-6-6348-3833
PCR kit for DNA amplification(Reagent for research)

2 HAZARDS IDENTIFICATION
Parts NamerTth DNA Polymerase
Most Important Hazards
rTth DNA Polymerase contain glycerol and may cause eye and skinSpecific Hazards
Not available
GHS Classification
Hazard class and category
Physical Hazards
Flammable liquids
Not classifiedHealth Hazards
Skin corrosion /Irritation
Category 3
Serious eye damage /Eye irritation
Category 2B
Environmental Hazards
Classification not possible
Label elements
Pictogram or symbol
Signal word
Warning
Causes mild skin irritation
Hazard statement
Causes eye irritation
Precautionary statement
Wash hands thoroughly after handlingResponse
If skin irritation occurs: Get medical advice/attention.
IF IN EYES: Rinse cautiously with water for several minutes. Remove
contact lenses if present and easy to do. Continue rinsing. If eyeirritation persists: Get medical advice/attention.
Parts Name
Most Important Hazards
Specific Hazards
GHS Classification

10x PCR Buffer and 2mM dNTPs
Few adverse human health effects are anticipated.
Not available
Classification not possible

2/5rTth DNA polymerase
Oct. 26, 2017

SDS No.1051F

Safety Data Sheet

3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical Product(Substance/Mixture)
Chemical Nature
Parts NameTTH-301

MixtureAqueous solution
CAS No. (EC NO.)
Main components
DNA polymerase(EC 2.7.7.7)
Tris(Hydroxymethyl)aminomethane 77-86-1
Poly(oxyethylene) octylphenyl ether 9002-93-1
56-81-5Glycerol
10×PCR BufferTris(Hydroxymethyl)aminomethane 77-86-1
Poly(oxyethylene) octylphenyl ether 9002-93-1
Dilution BufferTris(Hydroxymethyl)aminomethane 77-86-1
Poly(oxyethylene) octylphenyl ether 9002-93-1
56-81-5Glycerol2mM dNTPs
Deoxyadenosine triphosphate
1927-31-7
Deoxycytidine triphosphate
102783-51-7
Deoxyguanosine triphosphate
93919-41-6
Deoxythymidine triphosphate
18423-43-3
Components Contributing to the Hazard
Parts Name
TTH-301,Dilution Buffer
TTH-301,10×
PCRBuffer,Dilution Buffer
Common Chemical Name
GlycerolPoly(oxyethylene) octylphenyl(or Generic Name)
ether
Triton X-100
Synonyms
Glycerin
HO(C2H4O)n-C6H4-C8H17
Chemical formula
CH2OHCHOHCH2OH
CAS No.
56-81-59002-93-1
Concentration
50%
1.00%

4 FIRST-AID MEASURES
Inhalation

Skin Contact
Eye Contact

Ingestion

In case of irritation by inhaling this product, move affected person tofresh air and await recovery. If irritation persists, seek immediate
medical attention.
Wash with clean water, immediately.
Rinse cautiously with water a few minutes. Remove contact lenses if
present and easy to do. Continue rinsing. If eye irritation persists, seek
medical attention.
Induce vomiting.
If indisposition continues, seek medical attention.

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rTth DNA polymerase
Oct. 26, 2017

SDS No.1051F

5 FIRE-FIGHTING MEASURESExtinguishing Media
Protection of fire-fighters

Safety Data SheetWater, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side of fire area. Firefighters should wear proper protective equipment in case of large scalefire.

6 ACCIDENTAL RELEASE MEASURES
Personal Precautions
Environmental Precautions
Methods for Cleaning up

Wear protective gear to avoid eye/skin contact and inhalation.
Avoid disposition to the environment.
Use cloth, paper or anything similar to soak up the solution leaking outof the container. Take up under vacuum using dust collecting filter.

7 HANDLING AND STORAGE
HANDLING

Technical MeasuresPrecautions
Safe Handling Advice
STORAGE

Wear protective equipments and avoid contact with eyes and skin.
Handle with ventilation and local exhaust system.
Avoid substance contact .After handling, wash with clean water.
Good laboratory technique should be used when handling this product.

Technical Measures
Storage Conditions

Store in the laboratory bottle
Store at about -20°C

Incompatible Products
Packaging Materials

Oxidizers
Store in the original package

8 EXPOSURE CONTROLS/ PERSONAL PROTECTION
ENGINEERING
Control Parameter
Limit Values

JSOH OELACGIH TLVOSHA PEL

Set up good ventilation and exhaust system in the work area.
GlycerolNot established
310mg/m
3total dust:15mg/m TWA
respirable fr.: 5mg/m3TWAPERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection
Hand Protection
Eye Protection
Skin and Body Protection

Wear a dust mask when needed.
Wear Chemical safety gloves.
Wear protective eyeglasses or
Chemical safety goggles.
Wear lab coat when needed.

Poly(oxyethylene) octylphenylether
Not establishedNot establishedNot established4/5rTth DNA polymerase
Oct. 26, 2017

SDS No.1051F

Safety Data Sheet

9 PHYSICAL AND CHEMICAL PROPERTIES
Physical StateColourOdour
pH
Flash Point

LiquidcolourlessNone
7.0-9.0
No flammability for aqueous solution, but glycerol with the flash pointof 160℃ may remain after volatilization of water in rTth DNA
polymerase or Dilution Buffer.
Boiling Point
Not available
Melting Point
Not available
Decomposition Temperature Not available
3Density
1.0-1.2(g/cm )Solubility
Soluble in water

10 STABILITY AND REACTIVITY

Stability
Stable at below -20℃
Possible Hazardous ReactionsNothing particular.
Conditions to Avoid
High temperature,ignition
sources,direct sunlightMaterial to Avoid
Strong oxidizers and strong reducers
Not available
Hazardous Decomposition
Product11 TOXICOLOGICAL INFORMATION
Acute Toxicity
Not Available
Local Effects
May cause eye and skin irritation.
Toxicological information on the component of this product

Skin corrosion/Irritation
Serious eye damage/Eyeirritation

Poly(oxyethylene) octylphenyl ether
Glycerolrabbit:500mg/24hr:Mild
rabbit: Moderate irritation (Result of rabbit:500mg/24hr:MildHazard Assessment Report 200142(2002) conducted by Chemical
Evaluation and Research Institute inJapan)

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rTth DNA polymerase
Oct. 26, 2017

SDS No.1051F

Safety Data Sheet

12 ECOLOGICAL INFORMATION

Poly(oxyethylene) octylphenyl ether
EcotoxicityAcute toxicity (LC50)

Persistence/Degradability
Bioaccumulative potential
Mobility in Soil

Algae (Selenastrum)-0.21mg/l-96h (Result of Hazard Assessment
Report conducted by Ministry of the Environment and Chemical
Evaluation and Research Institute in Japan-2006)
Not rapidly degradable(28days,BOD 22%)
BCF<31,Not bioaccumulative
Not available

13 DISPOSAL CONSIDERATIONS
Waste from Residues

Contaminated Packaging

Dispose of in accordance with all applicable local and national laws andregulations.
Dispose of in accordance with all applicable local and national laws andregulations.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification Number Not classified
Follow all of the laws and regulations
Specific Precautions
To prevent packages from breaking,Store at about -20°C

15 REGULATORY INFORMATION

Follow all of the laws and regulations in your country.
Substances of very high concern (SVHC) according to REACH, Article
57.
9002-93-1 Triton X-100

16 OTHER INFORMATION
Notice

The information in this SDS, to the best of our knowledge, is accurate
and correct. However, TOYOBO makes no warranty and assumes noliability whatsoever in connection with any use of this information. The
SDS is subject to revision as new information becomes available.