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RT-PCR Quick Master Mix

Safety Data SheetFirst issue : May. 26, 2015
Revised: Dec 13, 2018
SDS No.1071F

1 PRODUCT AND COMPANY IDENTIFICATION
Product Name
Product Code
SUPPLIER
Name
AddressDepartment
Emergency Telephone No.
Fax No.
Recommended use andrestrictions on use

RT-PCR Quick Master Mix
PCR-311F
TOYOBO Co., Ltd.
2-2-8 Dojima Hama Kita-ku Osaka, 530-8230 Japan
Biotech support Department+81-6-6348-3786+81-6-6348-3833
Master mix for RT-PCR (Reagents for research)

2 HAZARDS IDENTIFICATION
Most Important Hazards

2x RT-PCR Quick Master Mix contain glycerol and may cause eyeand skin irritation.

Specific Hazard
Not available
GHS Classification
Hazard class and category

Physical Hazards
Health Hazards

Environmental Hazards

Flammable liquids
Skin corrosion /Irritation
Serious eye damage /Eyeirritation

Physical Hazards

Flammable liquids

Health Hazards

Skin corrosion /Irritation

Environmental Hazards

Physical Hazards
Health Hazards

Environmental Hazards
Label elements
Pictogram or symbol
Signal word
Hazard statement

Serious eye damage /Eyeirritation

2xRT-PCR Quick
Master Mix
Not classified

Classification notpossiblePositiveControl
Not classified

Manganese(Ⅱ)
acetateNot classified
Classification notpossibleClassification notpossibleClassification notpossible
Control Primer F
Not classified

Classification notpossibleClassification notpossibleClassification notpossibleClassification notpossibleClassification notpossibleClassification notpossible

Category 3
Category 2B

Control Primer R
Not classified
Classification notpossible
Serious eye damage /Eye Classification not
irritation
possibleClassification notpossible

Flammable liquids
Skin corrosion /Irritation

2xRT-PCR Quick Master Mix
Warning
Causes mild skin irritation
Causes eye irritation

Precautionary statement
Prevention
Wash hands thoroughly after handlingResponse

If skin irritation occurs: Get medical advice/attention.
IF IN EYES: Rinse cautiously with water for several minutes.
Remove contact lenses if present and easy to do. Continue rinsing.
If eye irritation persists: Get medical advice/attention.

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RT-PCR Quick Master Mix

Safety Data SheetFirst issue : May. 26, 2015
Revised: Dec 13, 2018
SDS No.1071F

3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical ProductChemical NatureParts Name
<2x RT-PCR Quick
Master Mix>

<50mM Mn(OAc)2>

MixtureAqueous solution
Main Component
N,N-Bis(2-hydroxyethyl)glycine
Potassium acetate
2'-Deoxyadenosine 5'-triphosphate
2'-Deoxycytidine 5'-triphosphate

CAS No.
150-25-4
127-08-2
1927-31-7
102783-51-7

2'-Deoxyguanosine 5'-triphosphate
Thymidine 5′-triphosphate
Bovine Serum Albumin
Immunoglobulin
DNA polymerase
GlycerolPolyethylene glycol tert-octylphenyl ether

93919-41-6
18423-43-3
56-81-59002-93-1

Manganese(Ⅱ) acetate638-38-1
7732-18-5

Water<RNase Free Water>
Components Contributing to the Hazard
Common Chemical Name
Glycerol(or Generic Name)
Synonyms
Chemical formulaGlycerin

CH2OHCHOHCH2OH(CH3COO)2Mn

56-81-5＜20%

638-38-0
0.9%（As forMn<0.3％)

CAS No.
Concentration

4 FIRST-AID MEASURESManganese(Ⅱ)
acetatePolyethylene glycol
tert-octylphenylether
Triton X-100
C8H17-C6H4O(C2H4O)nH
9002-93-1＜0.02%

Inhalation
Skin Contact

In case of irritation by inhaling this product, move affected person toWash with plenty of clean water, immediately.

Eye Contact

Rinse cautiously with water for several minutes. Remove contactlenses if present and easy to do. Continue rinsing. If eye irritationpersists: Get medical advice/attention.

Ingestion

Try to get the affected person to vomit as much as possible. Seek
medical attention, immediately.

5 FIRE-FIGHTING MEASURESExtinguishing Media
Protection of fire-fighters

Water, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side of fire area.
Fire-fighters should wear proper protective equipment in case oflarge scale fire.

6 ACCIDENTAL RELEASE MEASURES
Personal Precautions

Wear protective equipments and avoid eye/skin contact andinhalation.

Environmental Precautions

Avoid disposition to the environment.

Methods for Cleaning up

Use cloth, paper, or anything similar to soak up to the solution
leaking out of the container. Take up under vacuum using dust
collecting filter.

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RT-PCR Quick Master Mix

7 HANDLING AND STORAGE
HANDLING
Technical Measures

Precautions
Safe Handling Advice
STORAGEFirst issue : May. 26, 2015
Revised: Dec 13, 2018
SDS No.1071F

Safety Data Sheet

Wear protective equipments and avoid contact with eyes and skin.
Handle with ventilation and local exhaust system.
Avoid substance contact. After handling, wash hands completely.
Keep the handling area always clean.

Proper Storage Conditions Keep tightly closed and store at below -20℃
Packaging Materials
Store in the original package

8 EXPOSURE CONTROLS/ PERSONAL PROTECTION
ENGINEERING
Control Parameter
Limit Values

JSOH OEL

Set up good ventilation and exhaust system in the work area.

Glycerol (mist)

Manganese(Ⅱ)
acetatetetrahydrate

not established0.3mg/m3

Polyethylene glycol
tert-octylphenylether
not established

ACGIH TLVOSHA PEL

not established
10mg/m3
0.2mg/m3
total dust:
not established
not established
315mg/m TWA
respirable fr.:
35mg/m TWAPERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection
Wear a dust mask when needed.
Wear chemical safety gloves.
Hand Protection
Eye Protection
Wear protective eyeglasses or chemical safety goggles.
Skin and Body Protection

Wear lab coat when needed.

9 PHYSICAL AND CHEMICAL PROPERTIES
Physical State

ColourOdour
pH
Flash Point
Explosion limit
Density
Solubility

Liquid. Lysis Solution, Stop Solution, and Nuclease-free Water freeze
at -20℃.
ColourlessNone
6.0-9.0
No flammability due to aqueous solution
Not explosible
1.0～1.2(g/cm3)
Soluble in water

10 STABILITY AND REACTIVITY
Stability
Possible Hazardous
Conditions to Avoid
Material to Avoid
Hazardous Decomposition

Stable at below -20℃.
Nothing particularHigh temperature, ignition sources, direct sunlight
Strong oxidizer, strong reducers
Not available

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RT-PCR Quick Master Mix

Safety Data SheetFirst issue : May. 26, 2015
Revised: Dec 13, 2018
SDS No.1071F

11 TOXICOLOGICAL INFORMATION

Acute Toxicity
Not Available
Local Effects
May cause eye and skin irritation.
Toxicological information on the component of this product
GlycerolManganese(II)Polyethylene glycol
acetatetert-octylphenyl
tetrahydrate
ether
Oral-mouse: 4090mg/kg
Acute toxicity (LD50)
Oral-rat: 12.6g/kg
Oral-rat: 3730mg/kg Oral-rat: 1800mg/kg
Skin corrosion/Irritation
rabbit:500mg/24hr:Mild Serious eye damage/Eyerabbit:500mg/24hr:Mild
rabbit: Moderate
irritation
irritation (Result ofHazard Assessment
Report 200142(2002) conductedby Chemical
Evaluation andResearch Institute inJapan)

12 ECOLOGICAL INFORMATION

Ecotoxicological information on the components of this product
Poly(oxyethylene) octylphenyl ether
Persistence/Degradability
Bioaccumulation
Mobility
Acute aquatic toxicity(LC50)

Not available
Not available
Soluble in water and diffusible into water environment.
Algae (Selenastrum)-0.21mg/l-96h (Result of Hazard Assessment
Report conducted by Ministry of the Environment and Chemical
Evaluation and Research Institute in Japan-2006)

13 DISPOSAL CONSIDERATIONS
Waste from Residues

Contaminated Packaging

Dispose of in accordance with all applicable local and national laws
and regulations.
Dispose of in accordance with all applicable local and national laws
and regulations.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification Number
Specific Precautions

Not classified
Follow all of the laws and regulations in your respective country.
To prevent packages from breaking, handle with care.

15 REGULATORY INFORMATION

Follow all of the laws and regulations in your country.

16 OTHER INFORMATION
Notice

The information in this SDS, to the best of our knowledge, isaccurate and correct. However, TOYOBO makes no warranty andassumes no liability whatsoever in connection with any use of thisinformation. The SDS is subject to revision as new information
becomes available.