Cas9 Nuclease protein NLS (3 µg/µl)
Cas9 Nuclease protein NLS (15 µg/µl)
保存:－20°C
製品説明:
本 品 は 、 Streptococcus pyogenes 由 来 の Cas9Nuclease を、大腸菌で発現・精製した組換えタンパク質です。本品は核移行シグナル(NLS)を N 末端と C末端に有しており、ガイド RNA と組み合わせることによりゲノム編集に利用することができます。
製品内容:
Code No. 319-08641
構成品
Cas9 Nuclease protein NLS (3 µg/µL)

容量75 µg

Code No. 316-08651
構成品
Cas9 Nuclease protein NLS (15 µg/µL)

容量300 µg

形状:
10 mM Tris-HCl (pH 7.5), 300 mM NaCl, 0.1 mM
EDTA, 1 mM DTT, 50 % Glycerol
起源：
遺伝子組換え大腸菌

使用例:<in vitro での基質 DNA 切断チェック>
Cas9 Nuclease protein NLS
150 - 300 ng（約 1-2 pmol）
tracrRNA (2 µM)
1 µL
crRNA (2 µM)
1 µL↓
室温, 5 min.
↓
基質 DNA100 ng
10x H Buffer*1)
2 µL
d.d.H2O
up to 20 µL↓
37℃, 60 min.
↓Loading Buffer (SDS 含有) *2)↓65℃, 5 min.
↓
アガロースゲル電気泳動*1) 組成： 500 mM Tris-HCl (pH 7.9), 1 M NaCl,
100 mM MgCl2, 10 mM DTT*2) 正確な泳動結果を得るため、SDS を含む Loading Buffer(Code No.313-90111 など) の使用をお勧めします。

分子量：162,756 (アミノ酸配列より算出)
純度： 本酵素 10 µg と基質 DNA を 37℃で 1 時間反応させても、DNA のアガロースゲル電気泳動パターンに変化は認められない。
 本酵素 10 µg と基質 RNA を 37℃で 1 時間反応させても、RNA のアガロースゲル電気泳動パターンに変化は認められない。
 エンドトキシン ＜ 1 EU/µg

NIPPON GENE CO., LTD.
www.nippongene.com

本品は、試薬（試験研究用）として販売しているものです。
医薬品の用途には使用しないで下さい。

For Research Use OnlySA2107-2403

ラベルライセンス Label License：
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Cas9 Nuclease protein NLS 製品説明書
SA2107-2403