SYBR® Green Realtime PCR Master Mix
First issue : Jun. 25, 2014
SDS No.884F

Safety Data Sheet
1 PRODUCT AND COMPANY IDENTIFICATION
Product Name
Product Code
SUPPLIERNameAddressDepartment
Emergency Telephone No.
Fax No.
Recommended use andrestrictions on use

SYBR® Green Realtime PCR Master Mix
QPK-201,201T,209CH
TOYOBO Co., Ltd.
2-2-8 Dojima Hama Kita-ku Osaka, 530-8230 Japan
Biotech support Department+81-6-6348-3786+81-6-6348-3833
Master Mix for Realtime PCR

2 HAZARDS IDENTIFICATION

Most Important Hazards Few adverse human health effects are anticipated. Glycerol
whose flash point 160℃ is contained.
Specific Hazard
Not available
GHS classification
Classification not possible

3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical Product(Substance/Mixture)
Chemical Nature
Main components

MixtureAqueous solutions of enzyme, antibody and other compounds.
Concentration
CAS No.
(EC No.)(EC 2.7.7.7)
<0.1%
<0.1%

Taq DNA polymerase
Anti-Taq antibodies(monoclonal antibody)
Bovine Serum Albumin
Glycerol
56-81-5
Tris(hydroxymethyl)
77-86-1aminomethane7791-18-6
Magnesium chloride
Potassium chloride
7447-40-7®
163795-75-3SYBR Green I
ROX reference dye
deoxyadenosine 5'triphosphate
1927-32-7
deoxycytidine 5'triphosphate
102783-51-7
deoxyguanosine 5'triphosphate
93919-41-6
18423-43-3
deoxythymidine 5'triphosphate
Components Contributing to the Hazard
Common Chemical Name
GlycerolSynonyms
Glycerin
Chemical formula
CH2OHCHOHCH2
CAS No.
56-81-5
Concentration
2%

<0.1%
Ca. 2%<1%<1%<1%<0.001%<0.001%<1%SYBR® Green Realtime PCR Master Mix
First issue : Jun. 25, 2014
SDS No.884F

Safety Data Sheet
4 FIRST-AID MEASURES
Inhalation

Skin ContactEye Contact
Ingestion

In case of irritation by inhaling this product, move affected
person to fresh air and await recovery. Call a POISONCENTER or doctor if you feek unwell.
Wash with plenty of clean water, immediately.
Rinse cautiously with water for several minutes. Remove
contact lenses if present and easy to do. Continue rinsing. If
eye irritation persists: Get medical advice/attention
Try to get the affected person to vomit as much as possible.
Seek medical attention, immediately.

5 FIRE-FIGHTING MEASURESExtinguishing Media
Protection of fire-fightersWater, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side of fire
Fire-fighters should wear proper protective equipment incase of large scale fire.

6 ACCIDENTAL RELEASE MEASURES

Wear protective equipments and avoid eye/skin contact and inhal
Personal Precautions
Environmental Precautions Avoid disposition to the environment.
Use cloth, paper, or anything similar to soak up to theMethods for Cleaning up
solution leaking out of the container. Take up under vacuum
using dust collecting filter.

7 HANDLING AND STORAGE
HANDLING
Technical MeasuresWear protective equipments and avoid contact with eyes andskin. Avoid substance contact. After handling, wash hands
completely.
Handle with ventilation and local exhaust system.
Keep the handling area always clean.

Precautions
Safe Handling Advice
STORAGE
Proper Storage Conditions Store at -20℃. If it is for a few days, store at 4℃.
Packaging Materials
Store in the original packageSYBR® Green Realtime PCR Master Mix
First issue : Jun. 25, 2014
SDS No.884F

Safety Data Sheet
8 EXPOSURE CONTROLS/ PERSONAL PROTECTION

ENGINEERING MEASURES
Set up good ventilation and exhaust system in the work area.
Control Parameter
Glycerol
Limit Values
not established
JSOH OELACGIH TLV
10mg/m3
OSHA PEL
total dust: 15mg/m3TWA
respirable fr.: 5mg/m3TWAPERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection
Wear a dust mask when needed.
Hand Protection
Wear chemical safety gloves.
Wear protective eyeglasses or chemical safety goggles.
Eye Protection
Skin and Body Protection Wear lab coat when needed.

9 PHYSICAL AND CHEMICAL PROPERTIES
Physical State
ColourOdour
pH
Flash Point
Explosion limit
Density
Solubilityliquid. SYBR® Green Realtime PCR Master Mix freezes atless than -20℃.
very pale rednone
6.0-9.0
Not flammable due to aqueous solution, but glycerol whose
flash point 160℃ may stay behind after volatilization.
Not explosible
31.0-1.2(g/cm )Soluble in water.

10 STABILITY AND REACTIVITY
Stability
Possible HazardousReactions
Conditions to Avoid
Material to Avoid
Hazardous Decomposition
ProductStable at below -20℃.
Nothing particularHigh temperature, ignition sources, direct sunlight
Strong acids, strong alkalines and strong oxidizers
Not available

11 TOXICOLOGICAL INFORMATIONAcute Toxicity
Not available
May cause eye and skin irritation.
Local Effects
Toxicological information on the component of this product
GlycerolOral-mouse: 4090mg/kg
Acute toxicity (LD50)
Oral-rat: 12.6g/kg

SYBR® Green Realtime PCR Master Mix
First issue : Jun. 25, 2014
SDS No.884F

Safety Data Sheet
12 ECOLOGICAL INFORMATION
Mobility
Persistence/Degradability
Bioaccumulation

Soluble in water and diffusible into water environment.
Glycerol, enzyme, antibody, and deoxynucleotide arebiodegradable.
Not available

13 DISPOSAL CONSIDERATIONS
Waste from Residues

Contaminated Packaging

Dispose of in accordance with all applicable local andnational laws and regulations.
Dispose of in accordance with all applicable local andnational laws and regulations.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification Number Not classified
Follow all of the laws and regulations in your respective
To prevent packages from breaking, handle with care.
Specific Precautions

15 REGULATORY INFORMATION

Follow all of the laws and regulations in your country.

16 OTHER INFORMATION
Notice

The information in this SDS, to the best of our knowledge, isaccurate and correct. However, TOYOBO makes no warranty
and assumes no liability whatsoever in connection with any
use of this information.
The SDS is subject to revision as new information becomes
available.