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Hot Start TTx (RNA) Kit
First issue :Apr. 5, 2019
Revised : Apr. 1, 2021
SDS No.1691F

Safety Data Sheet
1 Chemical product and company identification
Hot Start TTx (RNA) Kit
Identification of the productProduct CodeHSTTX-111
SUPPLIERName
TOYOBO Co., Ltd.
Address
2-8 Dojima Hama 2-chome, Kita-ku OSAKA 530-8230 JAPANDepartment
Biotechnology Overseas Sales and Marketing Department+81-6-6348-3843
Emergency Telephone No.
+81-6-6348-3833
Fax No.
Recommended use and restrictions on usePCR Reagent (Reagent for research)

2 HAZARDS IDENTIFICATIONImportant Hazards
GHS Classification

Hazard class and category
Physical Hazards

Few adverse human health effects are anticipated.

Classification not possible

Health Hazards

5x Buffer forrTth/TTx(DNA/RNA)Hot Start TTx
DNAPolymerase

Acute Toxicity(Oral)

Not classified

Not classified

Skin corrosion/Irritation

Category 3

Category 3

Serious eye damage/Eye irritation
Environmental Hazards

Category 2B Category 2B
Classification not possible

Label elements<5x Buffer for rTth/TTx (DNA/RNA), Hot Start TTx DNA Polymerase>―Pictograms or symbols
WarningSignal word
Causes mild skin irritation.
Hazard statements
Causes eye irritation
Precautionary statements
Prevention
Response

Wash hands thoroughly after handling.
If skin irritation occurs: Get medical advice / attention.
If in eyes: Rinse cautiously with water for several minutes. Remove
contact lenses, if present and easy to do. Continue rinsing. If eyeirritation persists: Get medical advice / attention.

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Hot Start TTx (RNA) Kit
First issue :Apr. 5, 2019
Revised : Apr. 1, 2021
SDS No.1691F

Safety Data Sheet
3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical Product(Substance/Mixture)
Chemical Nature
Parts Name
<5x Buffer for rTth/TTx (DNA/RNA)><Hot Start TTx DNA Polymerase>

MixtureAqueous solution of enzyme, substrate, etc.
CAS No. (EC NO.)
Main components
Bicine(N,N‐Bis(2‐hydroxyethyl)glycine)
Potassium acetate

127-08-2

Polyethylene glycol mono-p-isooctylphenyl ether

9002-93-1

Glycerol

56-81-5

Tris(hydroxymethyl)aminomethan
ePotassium chloride

7447-40-7

DNA polymerase(EC 2.7.7.7)

Contained Parts:Percentage

Chemical formula
CAS No.

77-86-1Glycerol

56-81-5

Polyoxyethylene sorbitanmonolaurate

9005-64-5<50mM Mn(OAc)2>
Manganese(II) acetate
Components Contributing to the Hazard
Common Chemical Name
Polyethylene Glycerol(or Generic Name)
glycol mono-Synonyms

150-25-4

pisooctylphenyletherX-100, Glycerin
Triton638-38-0
Polyoxyethylen Manganese(e sorbitanII) acetatemonolaurate

Tween20
Polyethylene
Glycol-p(1,1,3,3tetramethylbutyl) phenyl
Ether
5x Buffer for 5x Buffer for Hot Start TTx
rTth/TTxrTth/TTx
DNA(DNA/RNA): (DNA/RNA), Polymerase:<0.1%
Hot Start TTx 0.5%
DNAPolymerase:≦50%
CH2OHCHOH C11H23COOC6
C8H17CH2OH
H8[O(CH2CH2C6H4OO)nH]
56-81-59005-64-5(CHO)nH9002-93-1

Mn(OAc)250mM
Mn(OAc)2:0.
9% (<0.3%as Mn)(CH3COO)2Mn
638-38-0

4 FIRST-AID MEASURES
Inhalation

In case of irritation by inhaling this product, remove person to fresh airand keep comfortable for breathing. Seek medical attention.

Skin Contact

Wash with clean water, immediately.
Take off contaminated clothing and wash before reuse.
If skin irritation or rash occurs: Get medical advice/ attention.
Rinse cautiously with water for several minutes. Remove contact lenses, if
present and easy to do. Continue rinsing.
If eye irritation persists: Get medical advice/ attention.
Rinse mouth.Induce vomiting.
If indisposition continues, seek medical attention.

Eye ContactIngestion

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Hot Start TTx (RNA) Kit
First issue :Apr. 5, 2019
Revised : Apr. 1, 2021
SDS No.1691F

Safety Data Sheet
5 FIRE-FIGHTING MEASURESExtinguishing MediaSpecific extinguishing methods
Protection of fire-fighters

Water, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side.
Fire-fighters should wear proper protective equipment in case of large
scale fire.

6 ACCIDENTAL RELEASE MEASURES
Personal Precautions
Environmental Precautions
Methods for Cleaning up

Wear protective gear to avoid eye/skin contact and inhalation.
Avoid disposition to the environment.
Take up under vacuum or soak up using cloth, paper or anything similar
and wash away the remainder with a large amount of water.

7 HANDLING AND STORAGE
HANDLING
Technical Measures
Precautions
Hygiene measures
STORAGE
Storage Conditions
Packaging Materials

Wear protective equipments and avoid contact with eyes and skin. Handle
with ventilation and local exhaust system.
Good laboratory technique should be used when handling this product.
After handling, wash with clean water.
Store at about -20°C
Store in the original package

8 EXPOSURE CONTROLS/ PERSONAL PROTECTION
ENGINEERING MEASURES
Control Parameter
Occupational exposure limit

JSOH OELACGIH TLVOSHAPELSet up good ventilation and exhaust system in the work area.
Polyethylene
glycol mono-pisooctylphenyl
ether

GlycerolPolyethylene glycol
mono-pisooctylphenyl ether

not establishedNot established

not established0.2mg/m3

not established3

not established

0.02mg/m3(resp.), 0.1mg/m3(IHL)(as inorganic manganesecompounds)
5mg/m3(as manganese andmanganase compounds)

not established

10mg/mTotal dust:
15mg/m3TWA
Respirable fr.:

not establishedPERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection
Wear a dust mask.
Chemical safety gloves.
Hand Protection
Chemical safety goggles.
Eye Protection
Skin and Body Protection
Long sleeves to prevent contact with skin.

Manganese(II) acetate

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Hot Start TTx (RNA) Kit
First issue :Apr. 5, 2019
Revised : Apr. 1, 2021
SDS No.1691F

Safety Data Sheet
9 PHYSICAL AND CHEMICAL PROPERTIES
Physical StateColourOdour
pHFlash Point

Liquid. 5x Buffer for rTth/TTx (DNA/RNA) and 50mM Mn(OAc)2 freeze at-20℃. Hot Start TTx DNA Polymerase is liquid over -20℃.
None
None
7.0-9.0
Not flammable due to aqueous solution, Glycerol whose flash point 160℃
may also stay behind after volatilization.

Decomposition Temperature

Not available
Not available
Not available

Specific gravity

1.0-1.2(g/cm3)

Solubility

Soluble in water

Melting Point
Boiling Point

10 STABILITY AND REACTIVITY
Stability
Possible Hazardous Reactions

Conditions to Avoid
Incompatible materials
Hazardous Decomposition ProductStable at -20℃
None
Strong heat, direct sunlight
Strong oxidizers and strong reducers
Not available

11 TOXICOLOGICAL INFORMATION<5x Buffer for rTth/TTx (DNA/RNA)>
Acute Toxicity, etc.
Not available
Reproduct Toxicity
Not available
Skin corrosion/irritation
May cause skin irritation.
Serious eye damage/eye irritation
May cause eye irritation.
Toxicological information on the component of this product
GlycerolAcute toxicity (Oral)

Oral-mouse: 4090mg/kg
Oral-rat: 12.6g/kgSkin corrosion/irritation
Serious eye damage/eye irritation

Polyethylene glycol mono-pisooctylphenyl ether
Mouse LD50: 4773mg/kg
Oral-rat: 1800mg/kg

May cause skin irritation.

May cause skin irritation.

May cause eye irritation

May cause eye irritation<Hot Start TTx DNA Polymerase>
Not available
Acute Toxicity
May cause mild skin irritation.
Skin corrosion/irritation
Serious eye damage/eye irritation
May cause eye irritation.
Toxicological information on the component of this product
GlycerolOral-mouse: 4090mg/kg
Acute toxicity (LD50)
Oral-rat: 12.6g/kg
Skin corrosion/irritation
Causes mild skin irritation
Serious eye damage/eye irritation
Causes eye irritation

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Hot Start TTx (RNA) Kit
First issue :Apr. 5, 2019
Revised : Apr. 1, 2021
SDS No.1691F

Safety Data Sheet<50mM Mn(OAc)2>
Not available
Acute Toxicity
Skin corrosion/irritation
May cause skin irritation.
Serious eye damage/eye irritation
May cause eye irritation.
Toxicological information on the component of this product
Manganese(II) acetate
Oral-rat: 3.73g/kg
Acute toxicity (LD50)
Skin corrosion/irritation
May cause skin irritation.
Serious eye damage/eye irritation
May cause eye irritation.

12 ECOLOGICAL INFORMATION
Ecotoxicity

GlycerolPolyethylene glycol mono-pisooctylphenyl ether

Fish(Rainboutrout)：
Fish(Bluegill):
LC50(96hr) 54g/L , (Goldfish)： LC50(96hr) 3 mg/L
LC50(24hr)＞5g/L
Crustacea(Daphnia magna)：
EC50(24hr) ＞10g/L
Red algae：EC50(28hr) 4.6g/L

Persistence and degradability

Polyethylene Glycol Mono-p-isooctylphenyl Ether isn't biodegradable.
Enzyme, protein, and glycerol are biodegradable.

Bioaccumulative potential
Mobility in soil

Not bioaccumulative
Soluble in water and diffusible into water environment.

13 DISPOSAL CONSIDERATIONS
Waste from Residues

Dispose of in accordance with all applicable local and national laws andregulations.

Contaminated Packaging

Dispose of in accordance with all applicable local and national laws andregulations.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification Number
Specific Precautions

Not classified
Follow all of the laws and regulations in your respective country.
To prevent packages from breaking, handle with care.Store at about -20°C
when it is transported.

15 REGULATORY INFORMATION
Regulations, Evaluation, Authorization
and Restriction of Chemicals(EU)The following ingredients are included in SVHC(Candidate list ofauthorization)

Common Chemical nameConcentration or concentration range
Chemical fomula
CAS#Regulations

Polyethylene glycol mono-p-isooctylphenyl ether(Triton X-100)＜0.1% (W/W)
HO(C2H4O)n-C6H4-C8H179002-93-1
Follow all of the laws and regulations in your country.

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Hot Start TTx (RNA) Kit
First issue :Apr. 5, 2019
Revised : Apr. 1, 2021
SDS No.1691F

Safety Data Sheet
16 OTHER INFORMATION
NoticeIts use must be supervised by a technically qualified individual experienced
in handling potentially hazardous chemicals. The information in this SDS, tothe best of our knowledge, is accurate and correct. However, TOYOBO makes
no warranty and assumes no liability whatsoever in connection with any useof this information. The information shall not be taken as being all inclusive
and is to be used only a guide. All materials and mixtures may be present
unknown hazards and should be used with caution. The SDS is subject torevision as new information becomes available.