RNA-direct SYBR® Green Realtime PCR Master Mix
First issue : Jun. 27, 2014

SDS No:886F

Safety Data Sheet
1 PRODUCT AND COMPANY IDENTIFICATION
Product Name
Product Code
SUPPLIER
NameDepartment
Address
Emergency Telephone No.
Fax No.
Recommended use andrestrictions on use

RNA-direct SYBR® Green Realtime PCR Master Mix
QRT-201, QRT-201T
TOYOBO Co., Ltd.
Biotech support Department
2-2-8 Dojima Hama Kita-ku Osaka, 530-8230 Japan+81-6-6348-3786+81-6-6348-3833
realtime PCR kit

2 HAZARDS IDENTIFICATION
Most Important Hazards
Specific Hazards
GHS classification

Few adverse human health effects are anticipated. Glycerol
whose flash point 160℃ is contained.
Not available
Classification not possibleRNA-direct SYBR® Green Realtime PCR Master Mix
First issue : Jun. 27, 2014

SDS No:886F

Safety Data Sheet
3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical Product(Substance/Mixture)
Chemical Nature
Parts Name

MixtureAqueous solutions of enzyme, antibody and other compounds.
Concentration
Main components
CAS No.
(EC No.)®
DNA polymerase<1%<RNA-direct SYBRBovineSerumAlbumin<1%Green Realtime PCR
Monoclonalantibody<1%Master Mix>
Bicine 〔N,N‐Bis(2‐150-25-4<1%hydroxyethyl)glycine〕
Potassium Acetate
127-08-2<1%9002-93-1≦0.02%Polyethylene Glycol Mono-pisooctylphenyl EtherGlycerol
56-81-5<20%
1927-31-7
deoxyadenosine 5'triphosphate<1%102783-51-7
deoxycytidine 5'triphosphate
93919-41-6
deoxyguanosine 5'triphosphate
18423-43-3
deoxythymidine 5'triphosphate®
163795-75-3<0.01%SYBR GreenI<50mM Mn(OAc)2>
Manganese(II) acetate638-38-050mMComponents Contributing to the Hazard
Common Chemical Name
GlycerolManganese(II) acetate
SynonymsGlycerin
Contained Parts
50mM Mn(OAc)2
RNA-directTM SYBR® Green
Realtime PCR Master Mix(CH3COO)2Mn
Chemical formula
CH2OHCHOHCH2
CAS No.
Concentration
Common Chemical Name
Synonyms

Contained Parts
Chemical formula
CAS No.
Concentration

56-81-5
2%
Polyethylene Glycol Mono-pisooctylphenyl Ether
Triton X-100, Polyethylene
Glycol-p-(1,1,3,3tetramethylbutyl) phenyl
Ether®
RNA-direct SYBR Green
Realtime PCR Master Mix
C8H17-C6H4O-(C2H4O)nH
9002-93-1≦0.02%

638-38-00.9%(<0.3% as Mn)

RNA-direct SYBR® Green Realtime PCR Master MixFirst issue : Jun. 27, 2014

SDS No:886F

Safety Data Sheet
4 FIRST-AID MEASURES
Inhalation

Skin Contact
Eye ContactIngestion

In case of irritation by inhaling this product, move affected
person to fresh air and await recovery. Call a POISONCENTER or doctor if you feek unwell.
Wash with clean water, immediately.
Rinse cautiously with water for several minutes. Remove
contact lenses if present and easy to do. Continue rinsing. If
eye irritation persists: Get medical advice/attention
Induce vomiting.
If indisposition continues, seek medical attention.

5 FIRE-FIGHTING MEASURESExtinguishing Media
Protection of fire-fighters

Water, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side of fire
Fire-fighters should wear proper protective equipment incase of large scale fire.

6 ACCIDENTAL RELEASE MEASURES

Wear protective equipments and avoid eye/skin contact and inhal
Personal Precautions
Environmental Precautions Avoid disposition to the environment.
Use cloth, paper, or anything similar to soak up to theMethods for Cleaning up
solution leaking out of the container. Take up under vacuum
using dust collecting filter.

7 HANDLING AND STORAGE
HANDLING
Technical Measures

Precautions
Safe Handling Advice
STORAGE
Storage Conditions
Packaging Materials

Avoid substance contact. Wear protective equipments andavoid contact with eyes and skin. After handling, wash hands
completely.
Handle with ventilation and local exhaust system.
Keep the handling area always clean.
Store at about -20℃
Store in the original package

RNA-direct SYBR® Green Realtime PCR Master Mix
First issue : Jun. 27, 2014

SDS No:886F

Safety Data Sheet
8 EXPOSURE CONTROLS/ PERSONAL PROTECTION

ENGINEERING MEASURESSet up good ventilation and exhaust system in the work area.
Control Parameter
Glycerol
Ｌｉｍｉｔ Ｖａｌｕｅｓ
Manganese(II) acetate
3
JSOH OEL
not established0.2mg/m3
ACGIH TLV
10mg/m0.2mg/m3
OSHA PEL
not establishedtotal dust: 15mg/m3TWA

respirable fr.: 5mg/m3TWA
Limit Values
Polyethylene Glycol Mono-pisooctylphenyl Ether
not established
JSOH OEL
not established
ACGIH TLV
not established
OSHA PELPERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection
Wear a dust mask.
Hand Protection
Chemical safety gloves.
Eye Protection
Chemical safety goggles.
Skin and Body Protection Long sleeves to prevent contact with skin.

9 PHYSICAL AND CHEMICAL PROPERTIES
Physical StateColourOdour
pHFlash PointDensity
Solubility

liquid RNA-direct Realtime PCR Master Mix and 50mM
Mn(OAc)2 freezes at less than -20℃.
colourlessnone
7.0～9.0
Not flammable due to aqueous solution, but glycerol whose
flash point 160℃ in RNA-direct Realtime PCR Master Mix
may stay behind after volatilization.
3
1.0-1.2(g/cm )Soluble in water.

10 STABILITY AND REACTIVITY
Stability
Possible Hazardous
Reactions
Conditions to Avoid
Material to Avoid
Hazardous Decomposition
ProductStable at less than -20℃.
Nothing particularHigh temperature, ignition sources, direct sunlight
Strong acids, strong alkalines and strong oxidizers
Not available

RNA-direct SYBR® Green Realtime PCR Master Mix
First issue : Jun. 27, 2014

SDS No:886F

Safety Data Sheet
11 TOXICOLOGICAL INFORMATION

Acute Toxicity
Not available
Reproduction Toxicity
Not available
May cause eye and skin irritation.
Local Effects
Toxicological information on the component of this product
GlycerolOral-mouse: 4090mg/kg
Acute toxicity (LD50)
Oral-rat: 12.6g/kg
Polyethylene Glycol Mono-pisooctylphenyl Ether
Oral-rat: 1800mg/kg
Acute toxicity (LD50)
Rabbit-skin: ≧3g/kg

Manganese(II) acetate
Oral-rat: 2.94g/kg

12 ECOLOGICAL INFORMATION
Mobility
Persistence/Degradability
Bioaccumulation

Soluble in water and diffusible into water environment.
Glycerol, enzyme, antibody, and deoxynucleotide arebiodegradable.
Not available

13 DISPOSAL CONSIDERATIONS
Waste from Residues

Contaminated Packaging

Dispose of in accordance with all applicable local andnational laws and regulations.
Dispose of in accordance with all applicable local andnational laws and regulations.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification Number Not classified
Follow all of the laws and regulations in your respective
To prevent packages from breaking, handle with care.
Specific Precautions

15 REGULATORY INFORMATION

Follow all of the laws and regulations in your country.

16 OTHER INFORMATION
Notice

The information in this SDS, to the best of our knowledge, isaccurate and correct. However, TOYOBO makes no warranty
and assumes no liability whatsoever in connection with any
use of this information.
The SDS is subject to revision as new information becomes
available.