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Genetic lineage and diversity of Fagus crenata trees planted in Shizuoka Prefecture
Hideyuki Katai*,1,5, Shinya Yamada1, Koichi Hiraoka2, Takeshi Hoshikawa1,
Nobuhiro Tomaru3 and Makoto Takahashi4´|ÈÂgJ½ #20ÁVÆÁVÇ%c»°«B DNA 4&)6*-5&.ÆSSRsÇ
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(:7:/È4&)6*-5&.UqCI¿@°«B DNA 136,&3
Abstract: The genetic lineage and diversity of Fagus crenata plantations established in Shizuoka Prefecture were surveyed byanalyzing chloroplast DNA and nuclear microsatellites (nSSRs). Chloroplast DNA haplotypes, which are naturally distributed along
the Sea of Japan side but not within the prefecture, were found in three out of the eight surveyed plantations. Two of the threeplantations were also classified as the same lineage as the Sea of Japan by a STRUCTURE analysis of nSSR genotypes. Therefore,
the two genetic markers used in this study revealed that the two plantations belonged to a different genetic lineage from that of thenatural populations found within the prefecture (i.e. the lineage found on the Pacific Ocean side). It was concluded that these twoplantations would not be suitable for the purpose of vegetation restoration from a genetic point of view. Furthermore, because of theestimated genetic diversity of the plantations using the nSSR loci, the values of allelic richness for all three plantations were lower than
the average value of the natural populations in the prefecture. Therefore, we suggest that more genetically diverse seeds and seedlings
are necessary to ensure more genetically diverse plantations.
Keywords: nuclear simple sequence repeat, planted trees, vegetation restoration, conservation genetics, chloroplast DNA haplotype*

E-mail: hideyuki1_katai@pref.shizuoka.lg.jp1

Âg¼ x³¤w 9 ¤+8,: Forestry and Forest Products Research Center, Shizuoka Prefectural

Research Institute of Agriculture and Forestry, 2542-8 Negata, Hamakita, Hamamatsu, Shizuoka 434-0016, Japan2

ÅJ20+8,: Kuromatsunai Buna Center, 512-1 Kuromatsunai, Kuromatsunai, Suttsu, Hokkaido 048-0101,

Japan
3

SPd[`[`ÀT¼`¤¢ Graduate School of Bioagricaltural Sciences, Nagoya University, Furo-cho, Chikusa,

Nagoya, Aichi 464-8601, Japan4

 ªR¤w ­£+8,: Forest Tree Breeding Center, Forestry and Forest Products Research Institute, 3809-1

Ishi, Juo, Hitachi, Ibaraki 319-1301, Japan5weÈÂg¼ x³¤w¯¤+8,: Tea Research Center, Shizuoka Prefectural Research Institute ofAgriculture and Forestry, 1706-11 Kurasawa, Kikugawa, Shizuoka 439-0002, Japan
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