1/5TM

ReverTra Ace qPCR RT MasterMix with gDNA RemoverFirst issue : Dec. 1, 2011

MSDS No.553F

Material Safety Data Sheet
1 PRODUCT AND COMPANY IDENTIFICATION
Product Name
Product Code
SUPPLIERNameAddress
Department
Emergency Telephone No.

Fax No.
Recommended use andrestrictions on use

ReverTra AceTM qPCR RT Master Mix with gDNA Remover
FSQ-301, 301STOYOBO Co., Ltd.
2-2-8 Dojima Hama Kita-ku Osaka, 530-8230 Japan
Biotech support Department+81-6-6348-3786+81-6-6348-3833
cDNA Synthesis Kit for Real-Time PCR(Reagent for research)

2 HAZARDS IDENTIFICATION
Most Important Hazards
Specific Hazards
GHS Classification

Few adverse human health effects are anticipated.
Not available
Classification not possible

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TM

ReverTra Ace qPCR RT MasterMix with gDNA RemoverFirst issue : Dec. 1, 2011

MSDS No.553F

Material Safety Data Sheet
3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical Product(Substance/Mixture)
Chemical Nature
Parts Name
gDNA Remover

MixtureAqueous solution of enzyme, substrate, etc.
CAS No. (EC NO.)
Main components
56-81-5GlycerolDeoxyribonuclease I
4×DN Master Mix
Tris(Hydroxymethyl)aminomethane 7786-30-3
Magnesium chloride
7786-30-356-81-5
GlycerolRibonuclease Inhibitor
5×RT Master Mix II
Tris(Hydroxymethyl)aminomethane 77-86-1
Potassium chloride
7447-40-7
Deoxyadenosine triphosphate
1927-31-7
Deoxycytidine triphosphate
102783-51-7
Deoxyguanosine triphosphate
93919-41-6
Deoxythymidine triphosphate
18423-43-3
Poly(oxyethylene) Nonylphenylether 9016-45-9
56-81-5GlycerolReverse transcriptase
9068-38-6
Deoxyribonucleic acid(the same as 5×RT Master Mix II but no Reverse transcriptase
5×RT Master Mix IIcontained)
no-RT Control
Nuclease-free Water
WaterComponents Contributing to the Hazard
Poly(oxyethylene)
Common Chemical Name
GlycerolNonylphenylether(or Generic Name)
Nonidet P-40
Synonyms
Glycerin
Contained
gDNA Remover：50%
5×RT Master Mix II, 5×RT
Parts:Percentage
4×DN Master Mix, 5×RT Master Mix Master Mix II no-RT Control ：II, 5×RT Master Mix II no-RT
0.0025%Control ：40%
Chemical formula
Not available
CH2OHCHOHCH2OH
CAS No.
56-81-59016-45-9

4 FIRST-AID MEASURES
Inhalation

Skin Contact
Eye Contact
Ingestion

In case of irritation by inhaling this product, move affected person tofresh air and await recovery. If irritation persists, seek immediate
medical attention.
Wash with clean water, immediately.
Flush eyes with clean water for more than 15 minutes, and seek medicalattention.
Induce vomiting.
If indisposition continues, seek medical attention.

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TM

ReverTra Ace qPCR RT MasterMix with gDNA RemoverFirst issue : Dec. 1, 2011

MSDS No.553F

Material Safety Data Sheet
5 FIRE-FIGHTING MEASURESExtinguishing Media
Protection of fire-fighters

Water, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side of fire area. Firefighters should wear proper protective equipment in case of large scale
fire.

6 ACCIDENTAL RELEASE MEASURES
Personal Precautions
Environmental Precautions
Methods for Cleaning up

Wear protective gear to avoid eye/skin contact and inhalation.
Avoid disposition to the environment.
Use cloth, paper or anything similar to soak up the solution leaking outof the container. Take up under vacuum using dust collecting filter.

7 HANDLING AND STORAGE
HANDLING
Technical Measures
Precautions
Safe Handling Advice
STORAGETechnical Measures
Storage Conditions
Incompatible Products
Packaging MaterialsWear protective equipments and avoid contact with eyes and skin.
Handle with ventilation and local exhaust system.
Avoid substance contact. After handling, wash with clean water.
Good laboratory technique should be used when handling this product.
Store in the laboratory bottle
Store at about -20°C
Oxidizers
Store in the original package

8 EXPOSURE CONTROLS/ PERSONAL PROTECTION
ENGINEERING MEASURES Set up good ventilation and exhaust system in the work area.

Control Parameter
Limit Values
JSOH OELACGIH TLVOSHA PEL

GlycerolNot established
10mg/m3
3
total dust:15mg/m TWA3respirable fr.: 5mg/m TWAPERSONAL PROTECTIVE EQUIPMENT

Respiratory Protection
Hand Protection
Eye Protection
Skin and Body Protection

Wear a dust mask.
Chemical safety gloves.
Chemical safety goggles.
Long sleeves to prevent contact with skin.

Poly(oxyethylene)
Nonylphenylether
Not establishedNot establishedNot established

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TM

ReverTra Ace qPCR RT MasterMix with gDNA RemoverFirst issue : Dec. 1, 2011

MSDS No.553F

Material Safety Data Sheet
9 PHYSICAL AND CHEMICAL PROPERTIES
Physical StateColourOdour
pH
Flash Point

Boiling Point
Melting Point
Decomposition TemperatureDensity
Solubility

gDNA Remover, 4×DN Master Mix, 5×RT Master Mix II, 5×RT MasterMix II no-RT Control: Liquid at -20℃.
None
None
7.0-9.0
No flammability due to aqueous solution, but glycerol whose flash point
160℃ may stay behind after volatilization gDNA Remover, 4×DN MasterMix, 5×RT Master Mix II, 5×RT Master Mix II no-RT Control.
Not available
Not available
Not available
1.0-1.2
Soluble in water

10 STABILITY AND REACTIVITY
Stability

Might be degraded but not cause hazardous reaction at room temperature.

Possible Hazardous Reactions NoneConditions to Avoid
Material to Avoid
Hazardous Decomposition
ProductHigh temperature, ignition sources, direct sunlight
Strong oxidizers, strong reducers
Not available

11 TOXICOLOGICAL INFORMATION
Acute Toxicity
Local Effects

Not available
May cause eye and skin irritation. May cause respiratory and digestive
tract irritation.

12 ECOLOGICAL INFORMATION
Mobility
Persistence/Degradability
Bioaccumulation

Soluble in water and diffusible into water environment.
Not available
Not available

13 DISPOSAL CONSIDERATIONS
Waste from Residues
Contaminated Packaging

Dispose of in accordance with all applicable local and national laws andregulations.
Dispose of in accordance with all applicable local and national laws andregulations.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification Number

Specific Precautions

Not classified
Follow all of the laws and regulations in your respective country.
To prevent packages from breaking, handle with care.

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TM

ReverTra Ace qPCR RT MasterMix with gDNA RemoverFirst issue : Dec. 1, 2011

MSDS No.553F

Material Safety Data Sheet
15 REGULATORY INFORMATION
Follow all of the laws and regulations in your country.

16 OTHER INFORMATIONNoticeThis product is sold for research purposes only and is not required toappear on the TSCA inventory. It is not intended for food, drug,household, agricultural or cosmetic use. Its use must be supervised by atechnically qualified individual experienced in handling potentiallyhazardous chemicals. The information in this MSDS, to the best of ourknowledge, is accurate and correct. However, TOYOBO makes nowarranty and assumes no liability whatsoever in connection with any useof this information. The information shall not be taken as being all
inclusive and is to be used only a guide. All materials and mixtures may
be present unknown hazards and should be used with caution. TheMSDS is subject to revision as new information becomes available.