Jpn. J. Infect. Dis., 54, 2001

Laboratory and Epidemiology Communications

Salmonella Brandenburg and S. Corvallis Involved in a Food Poisonlng

Outbreak in a Hospital in Hyogo Prefecture
Kokichi Hamada* and Hidetaka T叫iDivision of Microbiology, Hyogo Prefectural Institute of Public Health,Arata‑cho 2‑1‑29, Hyogo‑ku, Kobe 652‑0032
Communicated by Takashi Kawamura(Accepted December 4, 200 1)

ln comparisonwith those caused by Salmonella serovar

patients who were hospitalized. We obtained four Salmonella

Enteritidis,food poisonings caused by serotypes S. Brandenburg

isolates from the lunch, three S. Brandenburg lSOlates fromor SI Corvallis have been.rare in Japan (1)A We experienced

three dishes (dish Nos. 1‑3) and one S. Corvallis isolatefrom
another dish (No. 4).

anOutbreak of food poISOnlng ln a hospital in Hyogo Prefecture

in August 200 1. The outbreak involved both serotypes･Among

The 1 3 isolates were tested f♭r sensitivities to ampicillin,

3 1 5 persons who ingested the suspected meal, seven persons

cefotaxime, kanamycln, gentamicin, streptomycln, tetraCyCline,of 66‑88 years of age developed symptoms such as abdominal

trimethoprlm, Ciplofloxacin, fosfomycln, Chloramphenicol,

pain and fever. Nine Salmonella specimens were isolated fromsu胞methoxazole‑trimethoprlm, and nalidixic acid by uslng

stool specimens of the seven patients and one asymptomatic

Sensi Disk (Nippon Becton Dickinson Co･, Ltd･, Tbkyo) (2)･

patient. Seven isolates were S. Brandenburg and two were S.

The isolates were all sensitive to the tested antibiotics. TheyCoⅣallis. The stool specimen of one patient contained bothwere ex.aminedusing PFGE (pulsed‑Geld gel electrophoresis)

organisms.

employlng a gene path typing system (Program No. 5 ; NipponBio‑Rad, Tokyo) in a manner reported previously (3). As
shown in Figure 1, the PFGE patterns ofXbaI‑ or BlnI‑
digested chromosomal DNAs were the same within each

A lunch served on a specific day was suspected as a caus‑
ative fわod, because the lunch was the only fわod that wasshared by a patient in the dialysIS Ouトclinic and the otherPerotype･ Thus it was clear that the infection was caused by*ComSpOnding author: Fax: +8ト78‑53 1 ‑7080

Ingestion of the meal served in the hospital which was195

Jpn. J. Infect. Dis., 54, 2001
dishes were contaminated by the two micro‑organisms.

The present observation communicated in Japanese appearedin Infectious Agents Surveillance Report (lASR), vol. 22,
November, 2001.

REFERENCES
1. National Institute of Infectious Diseases and Tuberculo‑

sis and Infectious Diseases Control Division, MinistryofHealth, Labour and We脆re (2001): Salmonellosis inJapan as of June 2000. Infect. Agents Surveillance Rep.,
21, 162'‑163'.
2･ Jorgensen, J･ H･, Tumidge, ∫. D. and Washington, ∫. A.

( 1 999): Antibacterial susceptbilitytests: dilution and disk
diffusion methods. p.1526‑1543. In P. R. Murray (ed.),Manual of Clinical Microbiology. 7th ed. AmericanFig･ I ･ PFGE patterns ofJ仏al‑ and BlnLdigests ofchromosomal DNAof Salmonella Brandcnburg and Corvallis isolates.

Lanes 117: Xbal digcsts･ Lanes 8114: Blnl digests.

Society f♭r Microbiology, Washington, D.C.

Lanc I, 8: S. Brandcnburg from patient No. I.

3l Hamada, K., Tsuji, H., Shimada, K. and Hosoda, Y.

Lane 2, 9: S. Brandenburgfrom patient No. 2.
Lanc 3, 10: S. Brandcnburg from dish N0. 1.

(1 999): Epidemiological analysis ofSalm?nella serovar

Lane 4, I I : S, Brandenburg from patient No. 8.

Enteritidis isolates from two food poISOnlng Outbreaks

Lane 5, 12: S. Corvallisfrom patient No. 7.
Lane 6, 13: S. Corvams from dish No. 4.
Lane 7, 14: S. Corvams from patient No. 4.

in the Hyogo Prefecture by pulsed‑field gel electrophore‑sis. Bull. Hyogo Prefect. Inst. Public Health, 34, 1 13‑
1 17 (in Japanese).

M:入ladder.

4･ Hamada, K･, Tsuji, H･, Masuda, K･ and UenTura, K･ (1999)‥

Outbreak of salmonellosis caused by ingestlOn Of cuttle丘shchips: epidemiological analysis by pulsed‑fleld gel elec‑

Contaminated by the two serotypes of Salmonella.

trophoresis. Jpn. J. Infect. Dis., 52, 53154.
5･ Tsuji, H･and Hamada, K･ (1999): Outbreak ofsalm?11ello‑

From the end of 1998 to early 1999, various parts of Japan
experienced food poISOnlng Caused by cuttlefish chips
contaminated by two kinds of rare serotypes of Salmonella

sis caused by ingestion of cuttlefish chips contamlnated(4,5)･ The present case is very similar to the previous one inby both Salmonella Chester and Salmonella Oranienburg.

that the outbreak was caused by the two rare Salmonella

Jpn. J. Infect. Dis., 52, 138‑139.

serotypes･ It is not clear, however, by which route the fわur196