1/8GenNext NGS Library Prep Kit
First issue : Aug. 24, 2018
SDS No.1589F

Safety Data Sheet
1 CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
Identification of the product

GenNext NGS Library Prep Kit

Product Code
SUPPLIERNameAddress

LPK-101, LPK-101T, LPK-101L

Department
Emergency Telephone No.
Fax No.
Recommended use andrestrictions on use

TOYOBO Co., Ltd.
Osaka Umeda Twin Towers South,1-13-1 Umeda Kita-ku,
Osaka 530-0001, Japan
Biotechnology Overseas Sales and Marketing Department+81-6-6348-3846+81-6-6348-3833
Library construction for next-generation sequencing (Reagents for research)

2 HAZARDS IDENTIFICATION

Most Important Hazards Few adverse human health effects are anticipated.
GHS Classification
Hazard class and category
End Repair and A- End Repair and Atailing Enzyme
tailing Buffer
Physical Hazards
Health Hazards

Environmental Hazards

Physical Hazards
Health Hazards

Ligation solution

Flammable liquids

Not classified

Not classified

Not classified

Acute toxicity - oral

Not classified

Not classified

Not classified

Skin corrosion/Irritation

Category 3

Classification notpossibleClassification notpossible

Serious eye
damage /EyeirritationCategory 2B

Classification notpossibleClassification notpossibleClassification notpossibleClassification notpossibleClassification notpossible

Library
Amplification
Master Mix
Not classified
Category 4

Flammable liquids
Acute toxicity - oral
Skin corrosion
Category 3/Irritation
Serious eye
damage /EyeNot classified
irritation
Classification not possible
Environmental Hazards
Label elements
Pictogram or symbol
Signal word
Hazard statement

End Repair and A-tailing Enzyme
Warning
Causes mild skin irritation
Causes eye irritation

Precautionary statement
Prevention
Wash hands thoroughly after handlingResponse
If skin irritation occurs: Get medical advice/attention.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contactlenses if present and easy to do. Continue rinsing.
If eye irritation persists: Get medical advice/attention.

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GenNext NGS Library Prep Kit
First issue : Aug. 24, 2018
SDS No.1589F

Safety Data Sheet(to be contenued)

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GenNext NGS Library Prep Kit
First issue : Aug. 24, 2018
SDS No.1589F

Safety Data Sheet
2 HAZARDS IDENTIFICATION (Continued)
Label elements
Pictogram or symbol
Signal wordHazard statement

Library Amplification Master Mix
Warning
Harmful if swallowed.
Causes mild skin irritation

Precautionary statement
Prevention
Wash hands thoroughly after handling.
Do not eat, drink or smoke when using this product.
Response
If skin irritation occurs: Get medical advice/attention.
IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
Disposal
Dispose of contents/container in accordance with local/regional/national andinternational regulation.

Label elements

End Repair and A-tailing Buffer
Ligation solution
Library Amplification Primer Mix
No

3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical ProductChemical Nature
Parts Name
End Repair and A-tailing
EnzymeEnd Repair and A-tailing
BufferLigation solution

Library Amplification
Master Mix

Library Amplification
Primer Mix

MixtureAqueous solution
Main ComponentTris(hydroxymethyl)aminomethane
Dithiothreitol
GlycerolDNA polymerase
Tris(hydroxymethyl)aminomethane
Magnesium chloride
Dithiothreitol
Deoxyadenosine triphosphate
Deoxycytidine triphosphate
Deoxyguanosine triphosphate
Deoxythymidine triphosphate
Adenosine-5'-triphosphate disodium saltTris(hydroxymethyl)aminomethane
Magnesium chloride
Dithiothreitol
Adenosine-5'-triphosphate disodium salt
Polyethylene glycol
Glycerol
T4 DNA Ligase
Tris(hydroxymethyl)aminomethane
Magnesium sulfate
Deoxyadenosine triphosphate
Deoxycytidine triphosphate
Deoxyguanosine triphosphate
Deoxythymidine triphosphate
Additive A
DNA polymerase
Monoclonal antibody
GlycerolPoly(oxyethylene) nonylphenylether
Deoxyribonucleic acid

CAS No. (EC No.)77-86-1
3483-12-3
56-81-5(EC 2.7.7.7)
77-86-1
7786-30-3
3483-12-3
1927-31-7
102783-51-7
93919-41-6
18423-43-3
51963-61-2
77-86-1
7786-30-3
3483-12-3
51963-61-2
25322-68-356-81-5(EC 6.5.1.1)
77-86-1
7487-88-9
1927-31-7
102783-51-7
93919-41-6
18423-43-3
Nondisclosure(EC 2.7.7.7)－56-81-5
9016-45-9－(to be continued)

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GenNext NGS Library Prep Kit
First issue : Aug. 24, 2018
SDS No.1589F

Safety Data Sheet
3 COMPOSITION / INFORMATION ON INGREDIENTS (Continued)
Components Contributing to the Hazard
Common Chemical Name
Glycerol(or Generic Name)

Poly(oxyethylene)nonylphenyl ether

Additive A

Library
Amplification
Master Mix

Synonyms
Parts containedGlycerin
End Repair and Atailing Enzyme
Ligation solution
Library
Amplification
Master Mix

Nonidet P-40
Library
Amplification
Master Mix

Chemical formula

CH2(OH)CH(OH)
CH2(OH)56-81-5
approximately
50%

Not specified

CAS No.
Concentration

4 FIRST-AID MEASURES

9016-45-9< 0.01%

Nondisclosure
Nondisclosure< 3%Inhalation

Remove person to fresh air and keep at rest in a position comfortable forbreathing. Consult a physician if you feel unwell.

Skin Contact

Wash with plenty of clean water, immediately.
If skin irritation occurs: Get medical advice/attention.
Rinse cautiously with water for several minutes. Remove contact lenses if
present and easy to do. Continue rinsing. If eye irritation persists: Get medicaladvice/attention.
Rinse mouth. Vomit if possible. Seek medical attention, immediately.

Eye Contact

Ingestion

5 FIRE-FIGHTING MEASURESExtinguishing MediaSpecific extinguishingmethods
Protection of fire-fighters

Water, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side of fire area.
Fire-fighters should wear proper protective equipment in case of large scale fire.

6 ACCIDENTAL RELEASE MEASURES

Wear protective equipments and avoid eye/skin contact and inhalation.
Personal Precautions
Environmental Precautions Avoid disposition to the environment.
Methods for Cleaning up

Aspirate under reduced pressure, or soak up with cloth, paper or the like, andcollect it in a disposal container.

7 HANDLING AND STORAGE
Handling
Technical Measures

Precautions
Safe Handling Advice

Wear protective equipments and avoid contact with eyes and skin. Handle withventilation and local exhaust system.
Avoid substance contact. After handling, wash hands completely.
Keep the handling area always clean.
Wash hands thoroughly after handling.

Storage
Proper Storage Conditions Keep tightly closed and store at below -20℃
Packaging Materials
Store in the original package

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GenNext NGS Library Prep Kit
First issue : Aug. 24, 2018
SDS No.1589F

Safety Data Sheet6/8
GenNext NGS Library Prep Kit
First issue : Aug. 24, 2018
SDS No.1589F

Safety Data Sheet
8 EXPOSURE CONTROLS/ PERSONAL PROTECTION
Permissible concentration
Occupational exposurelimit
JSOH OELACGIH TLVOSHA PEL

Engineering controls

Glycerol(mist)
not established
10mg/m3total dust:
15mg/m3TWA
respirable fr.:
5mg/m3TWA

Poly(oxyethylene)nonylphenyl ether
not established
not established
not established

Set up good ventilation and exhaust system in the work area.
Provide washing facilities nearby.

Personal protective equipment
Respiratory Protection

Wear a dust mask when needed.

Hand Protection

Wear chemical safety gloves.

Eye Protection

Wear protective eyeglasses or chemical safety goggles.

Skin and Body Protection

Wear lab coat when needed.

9 PHYSICAL AND CHEMICAL PROPERTIES
Physical StateLiquid. End Repair and A-tailing Buffer, Ligation Solution, Library
Amplification Master Mix, and Library Amplification Primer Mix freeze at -20℃.

ColourOdour
pHFlash PointExplosion limitDensity
Solubility

ColourlessNone
6.0-9.5
No flammability due to aqueous solution
Not explosive
1.0～1.2(g/cm3)
Soluble in water

10 STABILITY AND REACTIVITY
Stability
Possible Hazardous
Conditions to Avoid
Incompatible materials

Stable at below -20℃.
Nothing particularHigh temperature, ignition sources, direct sunlight
Strong oxidizer, strong reducers

Hazardous Decomposition
Products

Not available

11 TOXICOLOGICAL INFORMATION

Acute Toxicity
Not Available
Local Effects
May cause eye and skin irritation.
Toxicological information on the component of this productAcute toxicity (LD50)

GlycerolPoly(oxyethylene)
Nonylphenylether

Additive A*

Oral-mouse:
4090mg/kg
Oral-rat: 12.6g/kg

Oral-rat: 50mg/kg

Category 2A

Skin corrosion/Irritation

Category 3

Oral-mouse:
3500mg/kg
Oral-rat:
4190mg/kg
Category 3

Serious eye damage/Eyeirritation

Category 2B

Category 2A

Dermal-rat:
537mg/kg
Category 2

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GenNext NGS Library Prep Kit
First issue : Aug. 24, 2018
SDS No.1589FSafety Data Sheet*: Allied substance data

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GenNext NGS Library Prep Kit
First issue : Aug. 24, 2018
SDS No.1589F

Safety Data Sheet
12 ECOLOGICAL INFORMATION
Ecotoxicity

GlycerolFish(Rainbou trout): LC50(96hr) 54g/L(Gold fish): LC50(24hr)＞5g/L
Crustacea(Daphnia magna): EC50(24hr)＞10g/L
Algae(Red algae): EC50(28hr) 4.6g/L

Persistence and degradabilityGlycerol, enzymes and nucleic acid related components are biodegradable.
Bioaccumulative potential
Mobility in soil

Ingredients are concidered to have no bioaccumulative potential.
Soluble in water and diffusible into water environment.

13 DISPOSAL CONSIDERATIONS
Waste from Residues

Contaminated Packaging

Dispose of in accordance with all applicable local and national laws andregulations.
Dispose of in accordance with all applicable local and national laws andregulations.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification Number
Specific Precautions

Not classified as dangerous goods.
Follow all of the laws and regulations in your respective country.
To prevent packages from breaking, handle with care.

15 REGULATORY INFORMATION

Follow all of the laws and regulations in your country.

16 OTHER INFORMATION
NoticeIts use must be supervised by a technically qualified individual experienced inhandling potentially hazardous chemicals. The information in this SDS, to thebest of our knowledge, is accurate and correct. However, TOYOBO makes nowarranty and assumes no liability whatsoever in connection with any use of thisinformation. The information shall not be taken as being all inclusive and is tobe used only a guide. All materials and mixtures may be present unknown
hazards and should be used with caution. The SDS is subject to revision as new
information becomes available.