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SuperPrep TM II Cell Lysis Kit for qPCRFirst issue : Dec. 13, 2016
SDS No.1347F

Safety Data Sheet
1 CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
Identification of the product
SuperPrep TM II Cell Lysis Kit for qPCR
Product Code
SUPPLIER
Name
Address
Department

Emergency Telephone No.

Fax No.
Recommended use andrestrictions on use

SCQ-501TOYOBO Co., Ltd.
2-2-8 Dojima Hama Kita-ku Osaka, 530-8230 Japan
Biotech support Department+81-6-6348-3786+81-6-6348-3833
Cell lysis for qPCR (Reagents for research)

2 HAZARDS IDENTIFICATION
Most Important Hazards

gDNA Remover and RNase Inhibitor contain glycerol and may cause eyeand skin irritation. Lysis Solution contains detergent and may cause eyeirritation.
Not available

Specific Hazard
GHS Classification
Hazard class and category

gDNA Remover RNase Inhibitor
Lysis Solution
Flammable liquids Not classified
Not classified
Not classified
Skin corrosion
Category 3
Category 3
Not classified/Irritation
Serious eye damage
Category 2B
Category 2B
Not classified/Eye irritation
Environmental Hazards
Classification
Classification
Classification not
not possible
not possible
possibleLabel elements
gDNA Remover
RNase Inhibitor
Pictogram or symbol
Signal word
Warning
Causes mild skin irritation
Hazard statement
Causes eye irritation
Precautionary statement
Wash hands thoroughly after handling
Prevention
If skin irritation occurs: Get medical advice/attention.
Response
IF IN EYES: Rinse cautiously with water for several minutes. Remove
contact lenses if present and easy to do. Continue rinsing. If eye irritation
persists: Get medical advice/attention.
Physical Hazards
Health Hazards

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SDS No.1347F

Safety Data Sheet
3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical Product(Substance/Mixture)
Chemical Nature
Parts Name
Lysis Solution

MixtureAqueous solution
Main ComponentTris(hydroxymethyl)aminomethane
Detergent
Magnesium chloride
Calcium Chloride
Dithiothreitol
Proteinase K
GlycerolgDNA Remover
Deoxyribonuclease
Glycerol
RNase Inhibitor
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
RNase Inhibitor
Components Contributing to the Hazard
Common Chemical Name
Detergent
Glycerol4-(2(or Generic Name)hydroxyethyl)-1piperazineethan
e sulfonic acid
Synonyms
Glycerin
HEPES
Chemical formula
CH2(OH)CH(OHC8H18N2O4S)CH2(OH)
CAS No.
56-81-57365-45-9
Concentration< 0.1%
approximately
0.5%
50%

4 FIRST-AID MEASURES
Inhalation

Skin Contact
Eye Contact

Ingestion

CAS No.
77-86-1
7786-30-3
10043-52-4
3483-12-3
39450-01-6
56-81-59003-98-9
56-81-57365-45-9-

In case of irritation by inhaling this product, move affected person to fresh
air and await recovery. If irritation persists, seek immediate medical
attention.
Wash with plenty of clean water, immediately.
Rinse cautiously with water for several minutes. Remove contact lenses if
present and easy to do. Continue rinsing. If eye irritation persists: Get
medical advice/attention
Try to get the affected person to vomit as much as possible. Seek medical
attention, immediately.

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SuperPrep TM II Cell Lysis Kit for qPCRFirst issue : Dec. 13, 2016
SDS No.1347F

Safety Data Sheet
5 FIRE-FIGHTING MEASURESExtinguishing Media
Protection of fire-fighters

Water, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side of fire area.
Fire-fighters should wear proper protective equipment in case of large
scale fire.

6 ACCIDENTAL RELEASE MEASURES

Wear protective equipments and avoid eye/skin contact and inhalation.
Personal Precautions
Environmental Precautions Avoid disposition to the environment.
Use cloth, paper, or anything similar to soak up to the solution leaking out
Methods for Cleaning upof the container. Take up under vacuum using dust collecting filter.

7 HANDLING AND STORAGE
HANDLING
Technical Measures

Wear protective equipments and avoid contact with eyes and skin. Handle
with ventilation and local exhaust system.
Avoid substance contact. After handling, wash hands completely.
Keep the handling area always clean.

Precautions
Safe Handling Advice
STORAGE
Proper Storage Conditions Keep tightly closed and store at less than -20℃
Store in the original package
Packaging Materials

8 EXPOSURE CONTROLS/ PERSONAL PROTECTION
ENGINEERINGMEASURES
Control Parameter
Limit Values

Set up good ventilation and exhaust system in the work area.
Provide washing facilities nearby.
Detergent

Glycerol4-(2-Aminoethyl)
benzenesulfonyl
fluoridehydrochloride

JSOH OEL

not established

not established

not established

ACGIH TLV

not established

10mg/m3

not established

OSHA PEL

not establishedtotal dust:
15mg/m3TWA
respirable fr.:
5mg/m3TWA

not establishedPERSONAL PROTECTIVE EQUIPMENT
Wear a dust mask when needed.
Respiratory Protection
Wear chemical safety gloves.
Hand Protection
Wear protective eyeglasses or chemical safety goggles.
Eye Protection
Skin and Body Protection Wear lab coat when needed.

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SDS No.1347F

Safety Data Sheet
9 PHYSICAL AND CHEMICAL PROPERTIES
Physical StateColourOdour
pH
Flash PointExplosion limit
Density
Solubility

Liquid. Lysis Solution freeze at -20℃.
ColourlessNone
6.0-9.0
No flammability due to aqueous solution
Not explosive
1.0～1.2(g/cm3)
Soluble in water

10 STABILITY AND REACTIVITY
Stability
Possible Hazardous
Reactions
Conditions to Avoid
Material to Avoid
Hazardous Decomposition
Product

Stable at below -20℃.
Nothing particularHigh temperature, ignition sources, direct sunlight
Strong oxidizer, strong reducers
Not available

11 TOXICOLOGICAL INFORMATION

Not Available
Acute Toxicity
May cause eye and skin irritation.
Local EffectsToxicological information on the component of this product
Detergent
Glycerol
Skin corrosion/Irritation-Serious eye damage/Eyeirritation

Rabbit,undilluted,
OECD405 :Category 1

rabbit:500mg/24
hr:Mild
rabbit:500mg/24
hr:Mild

12 ECOLOGICAL INFORMATION
Mobility
Persistence/Degradability
Bioaccumulation

Soluble in water and diffusible into water environment.
Not available
Not available

13 DISPOSAL CONSIDERATIONS
Waste from Residues

Contaminated Packaging

Dispose of in accordance with all applicable local and national laws andregulations.
Dispose of in accordance with all applicable local and national laws andregulations.

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SDS No.1347F

Safety Data Sheet
14 TRANSPORT INFORMATIONInternational Regulations

UN Classification Number

Specific Precautions

Not classified
Follow all of the laws and regulations in your respective country.
To prevent packages from breaking, handle with care.

15 REGULATORY INFORMATION

Follow all of the laws and regulations in your country.

16 OTHER INFORMATION
NoticeThis product is sold for research purposes only . It is not intended for food,drug, household, agricultural or cosmetic use. Its use must be supervised
by a technically qualified individual experienced in handling potentiallyhazardous chemicals. The information in this SDS, to the best of ourknowledge, is accurate and correct. However, TOYOBO makes nowarranty and assumes no liability whatsoever in connection with any useof this information. The information shall not be taken as being all
inclusive and is to be used only a guide. All materials and mixtures may bepresent unknown hazards and should be used with caution. The SDS issubject to revision as new information becomes available.