TM

SuperPrep

Cell Lysis & RT Kit for qPCRFirst issue : Jul. 22, 2013
SDS No.771F

Safety Data Sheet
1 PRODUCT AND COMPANY IDENTIFICATION
Product Name
SuperPrep TM Cell Lysis & RT Kit for qPCR
Product Code
SUPPLIER
Name
Address
Department
Emergency Telephone No.
Fax No.
Recommended use andrestrictions on use

SCQ-101TOYOBO Co., Ltd.
2-2-8 Dojima Hama Kita-ku Osaka, 530-8230 Japan
Biotech support Department+81-6-6348-3786+81-6-6348-3833
Cell lysis and cDNA synthesis for qPCR (Reagents for research)

TM

SuperPrep

Cell Lysis & RT Kit for qPCRFirst issue : Jul. 22, 2013
SDS No.771F

Safety Data Sheet
2 HAZARDS IDENTIFICATION
Most Important Hazards

gDNA Remover, RNase Inhibitor, 5xRT Master Mix, and 5x RT MasterMix no-RT control contain glycerol and may cause eye and skinirritation. Lysis Solution contains detergent and may cause eye
Not available

Specific Hazard
GHS Classification
Hazard class and category

gDNA Remover RNase Inhibitor Lysis Solution
Not classified
Not classified
Flammable liquid Not classified
Skin corrosion
Category 3
Category 3
Not classified/Irritation
Serious eye
Category 2BCategory 2B
Not classified
damage /EyeEnvironmental Hazards
Classification
Classification
Classification
not possible
not possible
not possible
5xRT Master
5xRT Master
Stop Solution
Mix no-RT
Mixcontrol
Not classified
Not classified
Physical Hazards
Flammable liquid Not classifiedHealth Hazards
Skin corrosion
Classification
Category 3
Category 3/Irritation
not possible
Serious eye
Classification
Category 2B
Category 2B
damage /Eyenot possible
Environmental Hazards
Classification
Classification
Classification
not possible
not possible
not possible
Label elements
gDNA Remover
RNase Inhibitor
5xRT Master Mix
5xRT Master Mix no-RT control
Pictogram or symbol
WarningSignal word
Hazard statement
Causes mild skin irritation
Causes eye irritation
Precautionary statement
Prevention
Wash hands thoroughly after handlingResponse
If skin irritation occurs: Get medical advice/attention.
IF IN EYES: Rinse cautiously with water for several minutes. Remove
contact lenses if present and easy to do. Continue rinsing. If eyeirritation persists: Get medical advice/attention.
Physical Hazards
Health Hazards

TMSuperPrep

Cell Lysis & RT Kit for qPCRFirst issue : Jul. 22, 2013
SDS No.771F

Safety Data Sheet
3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical Product(Substance/Mixture)
Chemical Nature
Parts Name
Lysis Solution

gDNA Remover
Stop Solution

RNase Inhibitor

5xRT Master Mix

5xRT Master Mix no-RTcontrol

Nuclease-free Water

MixtureAqueous solution
Main ComponentTris(hydroxymethyl)aminomethane
Poly(oxyethylene) octylphenyl ether
Magnesium chloride
Calcium Chloride
Dithiothreitol
Proteinase K
GlycerolDeoxyribonuclease
Tris(hydroxymethyl)aminomethane
Ethylenebis(oxyethylenenitrilo)tetraacetic acid4-(2-Aminoethyl)benzenesulfonyl fluoridehydrochloride
DithiothreitolGlycerol
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
RNase Inhibitor
Tris(hydroxymethyl)aminomethane
Potassium chloride
Magnesium chloride2'-deoxyadenosine 5'-(tetrahydrogen triphosphate)2'-deoxycytidine 5'-(tetrahydrogen triphosphate)2'-deoxyguanosine 5'-(tetrahydrogen triphosphate)2'-deoxythymidine 5'-(tetrahydrogen triphosphate)Poly(oxyethylene)nonylphenyl ether
GlycerolReverse Transcriptase
RNase InhibitorDNA
Tris(hydroxymethyl)aminomethane
Potassium chloride
Magnesium chloride2'-deoxyadenosine 5'-(tetrahydrogen triphosphate)2'-deoxycytidine 5'-(tetrahydrogen triphosphate)2'-deoxyguanosine 5'-(tetrahydrogen triphosphate)2'-deoxythymidine 5'-(tetrahydrogen triphosphate)Poly(oxyethylene)nonylphenyl ether
Glycerol
RNase Inihibitor
DNA
Water

CAS No.
77-86-1
9036-19-5
7786-30-3
10043-52-4
3483-12-3
39450-01-6
56-81-59003-98-9
77-86-1
67-42-530827-99-7
3483-12-3
56-81-57365-45-9
77-86-1
7447-40-7
7786-30-3
1927-31-7
102783-51-7
93919-41-6
18423-43-3
9016-45-9
56-81-5
77-86-1
7447-40-7
7786-30-3
1927-31-7
102783-51-7
93919-41-6
18423-43-3
9016-45-9
56-81-57732-18-5

TM

SuperPrep

Cell Lysis & RT Kit for qPCRFirst issue : Jul. 22, 2013
SDS No.771F

Safety Data Sheet
3 COMPOSITION / INFORMATION ON INGREDIENTS(CONTINUED)
Components Contributing to the Hazard
Common Chemical Name Poly(oxyethylene)(or Generic Name)
octylphenyl ether

Ethylenebis(oxy 4-(2-Aminoethyl)
ethylenenitrilo)t benzenesulfonyl
etraacetic acid fluoridehydrochloride
AEBSF
Synonyms
Triton X-114
Glycerin
EGTA
NH2CH2CH2C6H
Chemical formula(C2H4O)nC14H22O CH2(OH)CH(OH
C14H24N2O10)CH2(OH)
4SO2F
CAS No.
9036-19-556-81-567-42-5
30827-99-7
Concentration
0.1%
approximately
2%
0.1%
50%
Common Chemical Name 4-(2-hydroxyethyl)- Poly(oxyethylen(or Generic Name)
1-piperazineethane e)nonylphenyl
sulfonic acidether
Synonyms
HEPES
Nonidet P-40
C8H18N2O4S
Not specified
Chemical formula
CAS No.
7365-45-9
9016-45-9
Concentration
0.5%< 0.005%

4 FIRST-AID MEASURES
Inhalation

Skin Contact
Eye Contact

Ingestion

GlycerolIn case of irritation by inhaling this product, move affected person tofresh air and await recovery. If irritation persists, seek immediate
medical attention.
Wash with plenty of clean water, immediately.
Rinse cautiously with water for several minutes. Remove contact lenses
if present and easy to do. Continue rinsing. If eye irritation persists: Get
medical advice/attention.
Try to get the affected person to vomit as much as possible. Seek
medical attention, immediately.

5 FIRE-FIGHTING MEASURESExtinguishing Media
Protection of fire-fighters

Water, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side of fire area.
Fire-fighters should wear proper protective equipment in case of large
scale fire.

6 ACCIDENTAL RELEASE MEASURES

Wear protective equipments and avoid eye/skin contact and inhalation.
Personal Precautions
Environmental Precautions Avoid disposition to the environment.
Methods for Cleaning up
Use cloth, paper, or anything similar to soak up to the solution leaking
out of the container. Take up under vacuum using dust collecting filter.

TM

SuperPrep

Cell Lysis & RT Kit for qPCRFirst issue : Jul. 22, 2013
SDS No.771F

Safety Data Sheet
7 HANDLING AND STORAGE
HANDLING
Technical Measures

Wear protective equipments and avoid contact with eyes and skin.
Handle with ventilation and local exhaust system.
Avoid substance contact. After handling, wash hands completely.
Keep the handling area always clean.

Precautions
Safe Handling Advice
STORAGE
Proper Storage Conditions Keep tightly closed and store at below -20℃
Store in the original package
Packaging Materials

8 EXPOSURE CONTROLS/ PERSONAL PROTECTION
ENGINEERINGMEASURES
Control Parameter
Limit Values

Set up good ventilation and exhaust system in the work area.
Provide washing facilities nearby.
Poly(oxyethylene)
octylphenyl etherEthylenebis(oxy 4-(2-Aminoethyl)
ethylenenitrilo)t benzenesulfonyl
etraacetic acid fluoride
JSOH OEL
not established
not established not established not established
not established not established
ACGIH TLV
not established
10mg/m3
total
dust:
not established not established
OSHA PEL
not established
315mg/m TWA
respirable
fr.: 5mg/m3TWA
Limit Values
4-(2-hydroxyethyl)- Poly(oxyethylen1-piperazineethane e)nonylphenyl
sulfonic acidether
JSOH OEL
not established
not established
ACGIH TLV
not established
not established
OSHA PEL
not established
not establishedPERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection
Wear a dust mask when needed.
Hand Protection
Wear chemical safety gloves.
Eye Protection
Wear protective eyeglasses or chemical safety goggles.
Skin and Body Protection Wear lab coat when needed.

9 PHYSICAL AND CHEMICAL PROPERTIES
Physical State

ColourOdour
pH
Flash PointExplosion limit
Density
Solubility

Glycerol(mist)

Liquid. Lysis Solution, Stop Solution, and Nuclease-free Water freeze at-20℃.
ColourlessNone
6.0-9.0
No flammability due to aqueous solution
Not explosible
31.0～1.2(g/cm )Soluble in waterTMSuperPrep

Cell Lysis & RT Kit for qPCRFirst issue : Jul. 22, 2013
SDS No.771F

Safety Data Sheet
10 STABILITY AND REACTIVITY
Stability
Possible Hazardous
Reactions
Conditions to Avoid
Material to Avoid
Hazardous Decomposition
ProductStable at below -20℃.
Nothing particularHigh temperature, ignition sources, direct sunlight
Strong oxidizer, strong reducers
Not available

11 TOXICOLOGICAL INFORMATION

Acute Toxicity
Not Available
Local Effects
May cause eye and skin irritation.
Toxicological information on the component of this product
Poly(oxyethylene)
Glyceroloctylphenyl ether
Skin corrosion/Irritation
rabbit:500mg/24
hr:Mild
Serious eye damage/Eye rabbit: Moderate
rabbit:500mg/24
irritation
irritation (Result of hr:MildHazard
Assessment Report
2001-42(2002)
conducted by
Chemical
Evaluation and12 ECOLOGICAL INFORMATION

Mobility
Soluble in water and diffusible into water environment.
Persistence/Degradability
Not available
Bioaccumulation
Not available
Ecotoxicological information on the components of this product
Poly(oxyethylene) octylphenyl ether
Acute aquatic toxicity
Algae (Selenastrum)-0.21mg/l-96h (Result of Hazard Assessment Report(LC50)
conducted by Ministry of the Environment and Chemical Evaluation andResearch Institute in Japan-2006)

13 DISPOSAL CONSIDERATIONS
Waste from Residues

Contaminated Packaging

Dispose of in accordance with all applicable local and national laws andregulations.
Dispose of in accordance with all applicable local and national laws andregulations.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification Number Not classified
Follow all of the laws and regulations in your respective country.
Specific Precautions
To prevent packages from breaking, handle with care.

TMSuperPrep

Cell Lysis & RT Kit for qPCRFirst issue : Jul. 22, 2013
SDS No.771F

Safety Data Sheet
15 REGULATORY INFORMATION

Follow all of the laws and regulations in your country.

16 OTHER INFORMATION
Notice

The information in this SDS, to the best of our knowledge, is accurate
and correct. However, TOYOBO makes no warranty and assumes noliability whatsoever in connection with any use of this information. TheSDS is subject to revision as new information becomes available.