1/7

GenNext™ Shin-RamDA-seq™ Single Cell Stranded Kit
Code：RML-101, RML-101T
March 15th, 2023
SDS :2185F

Safety Data Sheet
1 CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
Identification of the productProduct Code
SUPPLIERNameAddress
Department
Emergency Telephone No.
Fax No.
Recommended use andrestrictions on use

GenNext™ Shin-RamDA-seq™ Single Cell Stranded Kit
RML-101, RML-101T
TOYOBO Co., Ltd.
Osaka Umeda Twin Towers South,
1-13-1 Umeda, Kita-ku, Osaka 530-0001, Japan
Biotechnology Overseas Sales and Marketing Department+81-6-6348-3846+81-6-6348-3833
cDNA amplification and library preparation kit for qPCR (Reagents forresearch)

2 HAZARDS IDENTIFICATION
Most Important Hazards

RNase Inhibitor, gDNA Remover, rRNA Remover, RT-RamDA™ Enzyme
Mix, 2nd strand synthesis Enzyme, Fragmentase, End Repair and Atailing Enzyme, Library Amplification Master Mix contain glycerol andmay cause eye and skin irritation. Lysis Buffer contains detergent andmay cause eye irritation.
Not available

Specific Hazard
GHS Classification
Hazard class and category①Lysis Buffer
Physical Hazards
Health Hazards

Environmental Hazards

Flammableliquids
Skin corrosion/Irritation
Serious eye
damage /Eyeirritation⑥gDNA Remover

Not classified③RNaseInhibitor
Not classified

Not classified

Category 3

Category 3

Not classified

Category 2B

Category 2B

Not classified

Not classified⑦rRNA
Remover

Not classified
Not classified⑧RT-RamDA™
⑪2nd strand
Enzyme Mix synthesis Enzyme

Not classified
Category 3

Not classified
Category 3

Not classified
Category 3

Category 2B

Category 2B

Category 2B

Not classified⑬Fragmentase

Not classified
Category 3

Not classified⑮End Repair
and A-tailing
EnzymeNot classifiedCategory 3

Not classified⑰LibraryAmplification
Master Mix
Not classified
Category 5

Category 2B

Category 2B

Category 3

Environmental Hazards
Not classified
Label elements
RNase Inhibitor
gDNA RemoverrRNA Remover
RT-RamDA™ Enzyme Mix
2nd strand synthesis Enzyme
Fragmentase
End Repair and A-tailing Enzyme
Library Amplification Master Mix

Not classified

Not classified

Physical Hazards
Health Hazards

Environmental Hazards

Physical Hazards
Health Hazards

Flammable liquids

Skin corrosion/Irritation
Serious eye
damage /Eyeirritation

Flammable liquids

Skin corrosion/Irritation
Serious eye
damage /Eyeirritation

2/7

GenNext™ Shin-RamDA-seq™ Single Cell Stranded Kit
Code：RML-101, RML-101T
March 15th, 2023
SDS :2185F

Safety Data Sheet
2 HAZARDS IDENTIFICATION (CONTINUED)
Pictogram or symbol
Signal word
Hazard statement

Warning
Causes mild skin irritation
Causes eye irritation

Precautionary statement
Prevention
Wash hands thoroughly after handlingResponse
If skin irritation occurs: Get medical advice/attention.
IF IN EYES: Rinse cautiously with water for several minutes. Remove
contact lenses if present and easy to do. Continue rinsing. If eye irritation
persists: Get medical advice/attention.

3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical Product(Substance/Mixture)
Chemical Nature
Parts Name
①Lysis Buffer

MixtureAqueous solution
Main Component
Proteinase K
Surfactant A②Lysis Enhancer
Additive A
Glycerol③RNase Inhibitor
Dithiothreitol
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acidRNase Inhibitor
Water④Nuclease free waterTris(hydroxymethyl)aminomethane⑤RT-RamDA™ Buffer
Magnesium chloride2'-deoxyadenosine 5'-(tetrahydrogen triphosphate)2'-deoxycytidine 5'-(tetrahydrogen triphosphate)2'-deoxyguanosine 5'-(tetrahydrogen triphosphate)2'-deoxythymidine 5'-(tetrahydrogen triphosphate)
Glycerol⑥gDNA RemoverTris(hydroxymethyl)aminomethane
Deoxyribonuclease
Magnesium chloride
Calcium chloride
Glycerol⑦rRNA RemoverTris(hydroxymethyl)aminomethane
Sodium chloride
Polyethylene glycol mono-p-isooctylphenyl ether⑧ RT-RamDA ™ Enzyme Tris(hydroxymethyl)aminomethane
Mix
Poly(oxyethylene)nonylphenyl ether
GlycerolReverse Transcriptase
RNase Inhibitor
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
⑨ RT-RamDA ™ Primer Tris(hydroxymethyl)aminomethane
MixEthylenediaminetetraacetic aciddisodium salt
Deoxyribonucleic acid

CAS No.
39450-01-6
56-81-53483-12-3
7365-45-9
7732-18-5
77-86-1
7786-30-3
1927-31-7
102783-51-7
93919-41-6
18423-43-3
56-81-5
77-86-1
9003-98-9
7786-30-3
10043-52-4
56-81-5
77-86-1
7647-14-5
9002-93-1
77-86-1
9016-45-9
56-81-57365-45-9
77-86-1
139-33-3-3/7

GenNext™ Shin-RamDA-seq™ Single Cell Stranded Kit
Code：RML-101, RML-101T
March 15th, 2023
SDS :2185F

Safety Data Sheet
3 COMPOSITION / INFORMATION ON INGREDIENTS (CONTINUED)⑩2nd strand synthesis
Buffer

⑪2nd strand synthesis
Enzyme⑪2nd strand synthesis
Primer Mix

Tris(hydroxymethyl)aminomethane

Magnesium chloride2'-deoxyadenosine 5'-(tetrahydrogen triphosphate)2'-deoxycytidine 5'-(tetrahydrogen triphosphate)2'-deoxyguanosine 5'-(tetrahydrogen triphosphate)2'-deoxyuridine 5'-(tetrahydrogen triphosphate)Tris(hydroxymethyl)aminomethane
GlycerolEthylenediaminetetraacetic aciddisodium salt
DNA polymerase
Tris(hydroxymethyl)aminomethane

Ethylenediaminetetraacetic aciddisodium salt
Deoxyribonucleic acid
Fragmentase
Tris(hydroxymethyl)aminomethane
Ethylenediaminetetraacetic acid disodium salt
GlycerolSodium chloride
Polyethylene glycol mono-p-isooctylphenyl ether<End Repair and A-tailing Tris(hydroxymethyl)aminomethane
Enzyme>Dithiothreitol
GlycerolDNA polymerase<End Repair and A-tailing Tris(hydroxymethyl)aminomethane
Buffer>Magnesium chloride
Dithiothreitol2'-deoxyadenosine 5'-(tetrahydrogen triphosphate)2'-deoxycytidine 5'-(tetrahydrogen triphosphate)2'-deoxyguanosine 5'-(tetrahydrogen triphosphate)2'-deoxythymidine 5'-(tetrahydrogen triphosphate)
Adenosine-5'-Sodium Triphosphate<Ligation solution>Tris(hydroxymethyl)aminomethane
Magnesium chloride
Dithiothreitol
Adenosine-5'-Sodium Triphosphate
Glycerol
T4 DNA Ligase
Tris(hydroxymethyl)aminomethane<Library Amplification
Master Mix>
Magnesium sulfate2'-deoxyadenosine 5'-(tetrahydrogen triphosphate)2'-deoxycytidine 5'-(tetrahydrogen triphosphate)2'-deoxyguanosine 5'-(tetrahydrogen triphosphate)2'-deoxythymidine 5'-(tetrahydrogen triphosphate)
Addictive B
DNA polymerase
monoclonal antibody
GlycerolPolyethylene glycol mono-p-isooctylphenyl ether
Deoxyribonucleic acid
<Library Amplification
Primer Mix>

77-86-1
7786-30-3
1927-31-7
102783-51-7
93919-41-6
102814-08-4
77-86-1
56-81-5
139-33-3(EC 2.7.7.7)
77-86-1
139-33-3
77-86-1
139-33-3
56-81-57647-14-5
9002-93-1
77-86-1
3483-12-3
56-81-5(EC 2.7.7.7)
77-86-1
7786-30-3
3483-12-3
1927-31-7
102783-51-7
93919-41-6
18423-43-3
51963-61-2
77-86-1
7786-30-3
3483-12-3
51963-61-2
56-81-5(EC 6.5.1.1)
77-86-1
7487-88-9
1927-31-7
102783-51-7
93919-41-6
18423-43-3
非公開(EC 2.7.7.7)
56-81-59016-45-9-

4/7GenNext™ Shin-RamDA-seq™ Single Cell Stranded Kit
Code：RML-101, RML-101T
March 15th, 2023
SDS :2185F

Safety Data Sheet
3 COMPOSITION / INFORMATION ON INGREDIENTS(CONTINUED)
Components Contributing to the Hazard
Common Chemical Name
Surfactant A(or Generic Name)
Synonyms
Chemical formula
CAS No.
Concentration
Common Chemical Name(or Generic Name)
Synonyms
Chemical formula
CAS No.
Concentration

4 FIRST-AID MEASURES
Inhalation

Skin Contact
Eye Contact

Ingestion

Glycerol

AdditiveA-Glycerin
guanidinium
CH2(OH)CH(OHC2H6N4S)CH2(OH)593-84-0
56-81-5< 1%< approximately< 1%50%4-(2-hydroxyethyl)- Poly(oxyethylen Polyethylene
1-piperazineethane e)nonylphenyl glycol mono-psulfonic acidether
isooctylphenyl
Triton X-100,
HEPES
Nonidet P-40
C8H18N2O4S
Not specified
HO(C2H4O)n9002-93-1
7365-45-9
9016-45-9< 0.5%< 0.1%< 0.0001%

AdditiveB< 1%In case of irritation by inhaling this product, move affected person to fresh
air and await recovery. If irritation persists, seek immediate medicalattention.
Wash with plenty of clean water, immediately.If skin irritation
occurs:Get medical advice/attention.
Rinse cautiously with water for several minutes. Remove contact lenses if
present and easy to do. Continue rinsing. If eye irritation persists: Get
medical advice/attention.
Try to get the affected person to vomit as much as possible. Seek medical
attention, immediately.

5 FIRE-FIGHTING MEASURESExtinguishing MediaSpecific extinguishingmethods
Protection of fire-fighters

Water, Carbon Dioxide, Foam, Dry Chemical Powder
Fire-fighting should be done from the windward side of fire area.
Fire-fighters should wear proper protective equipment in case of large
scale fire.

6 ACCIDENTAL RELEASE MEASURES
Personal Precautions
Environmental Precautions

Wear protective equipments and avoid eye/skin contact and inhalation.
Avoid disposition to the environment.

Methods for Cleaning up

Use cloth, paper, or anything similar to soak up to the solution leaking
out of the container. Take up under vacuum using dust collecting filter.

7 HANDLING AND STORAGE
HANDLING
Technical Measures
Precautions
Hygine measures

Wear protective equipments and avoid contact with eyes and skin. Handle
with ventilation and local exhaust system.
Avoid substance contact.
Keep the handling area always clean.After handling, wash hands
completely.

STORAGE
Proper Storage Conditions Keep tightly closed and store at below -20℃
Packaging Materials
Store in the original package

5/7

GenNext™ Shin-RamDA-seq™ Single Cell Stranded Kit
Code：RML-101, RML-101T
March 15th, 2023
SDS :2185F

Safety Data Sheet
8 EXPOSURE CONTROLS/ PERSONAL PROTECTION
Control Parameter
Limit Values

Surfactant A

JSOH OELACGIH TLV

not established
not established

OSHA PEL

not established

Limit Values

Addictive B

Glycerol(mist)

4-(2hydroxyethyl)-1piperazineethan
e sulfonic acid

Addictive A

not established
10mg/m3
total dust:
15mg/m3TWA
respirable fr.:
5mg/m3TWA

not established
not established

not established
not established

not established

not established

Polyethylene glycol
mono-pisooctylphenyl
ether

Poly(oxyethylene)nonylphenylether
not established
not established
not established

JSOH OEL
not established
not established
ACGIH TLV
not established
not established
OSHA PEL
not established
not establishedPERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection
Wear a dust mask when needed.
Hand Protection
Wear chemical safety gloves.
Eye Protection
Wear protective eyeglasses or chemical safety goggles.
Skin and Body Protection Wear lab coat when needed.

9 PHYSICAL AND CHEMICAL PROPERTIES
Physical State

Liquid at normal temperature. Lysis Buffer, Lysis Enhnacer, RT-RamDA™ Buffer, RT-RamDA™ Enzyme Mix, RT-RamDA™ Primer Mix, 2ndstrand synthesis Buffer, 2nd strand synthesis Primer Mix, End Repair
and A-tailing Buffer, Ligation Solution, Library Amplification Master Mix
and Library Amplification Primer Mix Solution freeze at -20℃.

ColourOdour
pHFlash PointColourless
Odourless
6.0-9.0
Not flammable due to aqueous solution, but glycerol whose flash point
160°C may stay behind after volatilization.
Not explosive
1.0～1.2(g/cm3)
Soluble in waterExplosion limit
Density
Solubility

10 STABILITY AND REACTIVITY
Stability
Possible Hazardous
Reactions
Conditions to Avoid
Material to Avoid
Hazardous Decomposition
ProductStable at below -20℃.
Nothing particularHigh temperature, ignition sources, direct sunlight
Strong oxidizer, strong reducers
Not available

6/7

GenNext™ Shin-RamDA-seq™ Single Cell Stranded Kit
Code：RML-101, RML-101T
March 15th, 2023
SDS :2185F

Safety Data Sheet
11 TOXICOLOGICAL INFORMATION

Acute Toxicity
Not Available
Local Effects
May cause eye and skin irritation.
Toxicological information on the component of this productSurfactant A
GlycerolAcute toxicity (LD50)-Oral-mouse:
4090mg/kg
Oral-rat:
12.6g/kg
Category 3

Skin corrosion/Irritation-Serious eye damage/Eyeirritation

Rabbit, undilluted, Category 2B
OECD405:
Category 1

Acute toxicity (LD50)
Skin corrosion/Irritation

Polyethylene Poly(oxyethylene)
Glycol Mono-pisooctylphenyl
NonylphenyletherEtherOral-rat:
Oral-mouse:
1800mg/kg
3500mg/kg
Rabbit-skin: ≧ Oral-rat:
3g/kg
4190mg/kg
Category 3
Category 2
Category 2ACategory 2A

AdditiveA
AdditiveB
Oral-rat: 593mg/kg Oral-rat:
50mg/kg*
Category1C
Category 2

Category 1
Category 2A
Serious eye damage/Eyeirritation*Acute toxicity (LD50) of Additive is estimated from similar agent.

12 ECOLOGICAL INFORMATION
Ecotoxicity

Surfactant A

Rainboutrout:
LC50(96hr)＞0.5 mg/L

Glycerol

AddictiveA

Polyethylene
glycol mono-pisooctylphenylether
Fish(Bluegill):
LC50(96hr) 3mg/L

Rainboutrout:
Guppy:
LC50(96hr)
C50(96hr)
54g/L
89.1g/LGold fish:
Daphnia
LC50(24hr)＞magna50(48hr)
5g/L＞42.4g/L
Daphnia magna Green algae:: EC50(24hr)＞ EC50(72hr)
10g/L
130g/LRed algae:
EC50(28hr)
4.6g/L
Persistence and degradability Glycerol and enzyme or the like is Biodegradable.Surfactant A is notreadily biodegradable.
Bioaccumulative potential
Thought to be not bioaccumulative.
Mobility in soil
Soluble in water and diffusible into water environment.

13 DISPOSAL CONSIDERATIONS
Waste from ResiduesContaminated Packaging

Dispose of in accordance with all applicable local and national laws andregulations.
Dispose of in accordance with all applicable local and national laws andregulations.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification Number
Specific Precautions

Not classified
Follow all of the laws and regulations in your respective country.
To prevent packages from breaking, handle with care.

7/7

GenNext™ Shin-RamDA-seq™ Single Cell Stranded Kit
Code：RML-101, RML-101T
March 15th, 2023
SDS :2185F

Safety Data Sheet
15 REGULATORY INFORMATION

Follow all of the laws and regulations in your country.

16 OTHER INFORMATION
NoticeThis product is sold for research purposes only. It is not intended for food,drug, household, agricultural or cosmetic use. Its use must be supervised
by a technically qualified individual experienced in handling potentiallyhazardous chemicals. The information in this SDS, to the best of ourknowledge, is accurate and correct. However, TOYOBO makes nowarranty and assumes no liability whatsoever in connection with any useof this information. The information shall not be taken as being all
inclusive and is to be used only a guide. All materials and mixtures may
be present unknown hazards and should be used with caution. The SDS issubject to revision as new information becomes available.