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Ligation high Ver.2
First issue: May 27, 2015
SDS No. 1069F

Safety Data Sheet
1 PRODUCT AND COMPANY IDENTIFICATION
Product Name
Ligation high Ver.2
Product CodeLGK-201
SUPPLIERTOYOBO Co., Ltd.
NameAddress
2-2-8 Dojima Hama Kita-ku Osaka, 530-8230 Japan
Department
Biotech support Department
Emergency Telephone No. +81-6-6348-3786
Fax No.
+81-6-6348-3833
Recommended use andLigation reagent
restrictions on use(Reagent for research)

2 HAZARDS IDENTIFICATION
Important Hazards

Few adverse human health effects are anticipated.

GHS Classification
Hazard class and category
Physical Hazards
Not classified.
Health Hazards
Acute toxicity - oral
Skin corrosion/Irritation
Serious eye damage/Eye irritation
Environmental Hazards Classification not possible.

Classification not possible.
Category 3
Category 2B

Label elements
Pictograms or symbols
Signal word―
Warning
Causes mild skin irritation.
Hazard statements
Causes eye irritation.
Precautionary statement(s)Avoid substance contact. After handling, wash hands with clean
Prevention
If skin irritation occurs: Get medical advice / attention. If in eyes:
Response
Rinse cautiously with water for several minutes. Remove contactlenses, if present and easy to do. Continue rinsing.If eye irritation
persists: Get medical advice / attention.

3 COMPOSITION / INFORMATION ON INGREDIENTS
Chemical Product(Substance/Preparation)
Chemical Nature
Parts Name
Ligation high Ver.2

MixtureAqueous solution
CAS No. (EC NO.)
Main components
T4 DNA Ligase(EC 6.5.1.1)
Tris(Hydroxymethyl)aminomethane 77-86-1
Glycerol
56-81-5
Magnesium chloride
7786-30-3
Sodium chloride
7647-14-5
Dithiothreitol
3483-12-3

2/4Ligation high Ver.2
First issue: May 27, 2015
SDS No. 1069F

Safety Data Sheet
Components Contributing to the Hazard
Common Chemical Name
Glycerol(or Generic Name)
Synonyms
Glycerin
Chemical formula
CH2OHCHOHCH2OH
CAS No.
Concentration

56-81-5
approximately 10%

4 FIRST-AID MEASURES
Inhalation
Skin Contact
Eye Contact

Ingestion

Remove person to fresh air. Call a doctor if you feel unwell.
Wash with clean water, immediately.
Rinse cautiously with water for several minutes. Remove contactlenses, if present and easy to do. Continue rinsing.
If eye irritation persists: Get medical advice / attention.
If swallowed: Rinse mouth. Do NOT induce vomiting.
If indisposition continues, seek medical attention.

5 FIRE-FIGHTING MEASURESExtinguishing Media
Protection of fire-fighters

Water, Carbon Dioxide, Foam, Dry Chemical Powder.
Fire-fighting should be done from the windward side of fire area.
Fire-fighters should wear proper protective equipment in case of large
scale fire.

6 ACCIDENTAL RELEASE MEASURES
Personal Precautions
Wear protective gear to avoid eye/skin contact and inhalation.
Environmental Precautions Avoid disposition to the environment.
Methods for Cleaning up
Use cloth, paper or anything similar to soak up the solution leaking
out of the container. Take up under vacuum using dust collecting
filter.

7 HANDLING AND STORAGE
HANDLING
Technical Measures
Precautions
Safe Handling Advice
STORAGETechnical Measures
Storage Conditions
Incompatible Products
Packaging Materials

Wear protective equipments and avoid contact with eyes and skin.
Always keeping things tidy and in order, and keep laboratory Clean.
Avoid substance contact. After handling, wash hands with clean water.
Store in the laboratory bottle.
Store at about -20°C.
Oxidizers
Store in the original package.

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Ligation high Ver.2
First issue: May 27, 2015
SDS No. 1069F

Safety Data Sheet
8 EXPOSURE CONTROLS/ PERSONAL PROTECTION
ENGINEERINGControl Parameter
Limit Values
JSOH OELACGIH TLVOSHA PEL

Set up good ventilation and exhaust system in the work area.

GlycerolNot established
10mg/m3
total dust:15mg/m3TWA
3
respirable fr.: 5mg/m TWAPERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection
Wear a dust mask.
Hand Protection
Chemical safety gloves.
Eye Protection
Chemical safety goggles.
Skin and Body Protection Long sleeves to prevent contact with skin.

9 PHYSICAL AND CHEMICAL PROPERTIES
Physical StateColourOdour
pH
Flash Point
Explosion limit
Specific Gravity
Solubility

Liquid over -20℃.
ColourlessNone
6.0-9.0
Not flammable due to aqueous solution.
Not explosible.
1.0-1.2
Soluble in water.

10 STABILITY AND REACTIVITY
Stability
Possible Hazardous
Reactions
Conditions to Avoid
Material to Avoid
Hazardous Decomposition
Product

Stable at -20°C
None
Strong heat and direct sunlight.
Strong oxidizers and strong reducers.
No information available.

11 TOXICOLOGICAL INFORMATION
Acute Toxicity
Local Effects

No data available.
May cause eye and skin irritation. May cause respiratory tract
irritation.
Toxicological information on the component of this product
GlycerolAcute toxicity (LD50)
Oral-mouse: 4090mg/kg
Oral-rat: 12.6g/kg
rabbit:500mg/24hr:Mild (Category 3)
Skin corrosion/Irritation
Serious eye damage/Eyerabbit:500mg/24hr:Mild (Category 2B)
irritation

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Ligation high Ver.2
First issue: May 27, 2015
SDS No. 1069F

Safety Data Sheet
12 ECOLOGICAL INFORMATION
Mobility
Persistence/Degradability
Bioaccumulation

Soluble in water and diffusible into water environment.
Not available.
Not available.

13 DISPOSAL CONSIDERATIONS
Waste from Residues
Contaminated Packaging

Dispose of in accordance with all applicable local and national laws andregulations.
Dispose of in accordance with all applicable local and national laws andregulations.

14 TRANSPORT INFORMATIONInternational Regulations
UN Classification Number

Specific Precautions

Not classified.
Follow all of the laws and regulations in your respective country.
To prevent packages from breaking, handle with care. Store at about -20°
C when it is transported.

15 REGULATORY INFORMATION
Follow all of the laws and regulations in your country.

16 OTHER INFORMATION
Notice

The information in this SDS, to the best of our knowledge, is accurate
and correct. However, TOYOBO makes no warranty and assumes noliability whatsoever in connection with any use of this information. The
information shall not be taken as being all inclusive and is to be used
only a guide. All materials and mixtures may be present unknown
hazards and should be used with caution. The SDS is subject to revision
as new information becomes available.