Asian Journal of Management Sciences & Education Vol. 11(1) January-April 2022__________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________IN VITRO STUDY OF HAIR MASK/ HAIR CONDITIONERCONTAINING GOODNESS OF NATURAL ORIGIN INGREDIENTS TO
GET MAXIMUM BENEFITS
S.M Badiul Alam Siddiqui, Rahul Deshkar, Shaikh Nasir
Dabur Research & Development, International Business Development, Dubai,
UNITED ARAB EMIRATES.
badiul.alam@dabur.com, rahul.deshkar@dabur.com, nasir.shaikh@dabur.com

ABSTRACT
Hair Care categories are well growing and consumers are always keen to try betterand better alternatives in everyday life. There are different segments in category
shelves for different usages. Now these are segmenting further in organic, natural
origin and normal (Not claiming such). Main reason behind is consumer trends as
the days passes on and on, consumers are well educated on natural trends andbenefits of these products. Most of the consumers are happy to pay more to get highnatural content containing products. Primary need is beautiful hair in less time.
Today the consumer is too busy and they don’t want to spare time in these routines.
Anything can appeal them to spend money and give a try which is satisfying theirneed.
Common formats are, Hair Shampoos, Hair Conditioners, 2 in 1 Hair Shampoo andconditioner, Hair Masks. These ranges are rinse off as a main format types. Thereare leave on conditioners and hair masks also available in the market which is close
to the hair cream and serums. The purpose is to help with good hair and scalp
cleaning and conditioning to the consumer. The science is to use silicone, Cationicquaternary compounds and Triglycerides etc. to help get moistures entrapped. TheMechanism is very simple, wet hair carries negative charge and it helps cationiccompound to stick with hair.
Natural hair mask for rinse off and leave on is one of the best product to offer to theconsumers for their better hair and scalp health. It is full of natural origin goodness
and contains fruit extracts, natural triglycerides, signature Vatika oil blends andquaternary compounds to help consumer embedding best ever experiences for day today need.
This formula also help lock moisture for long and help keep hair prevent fromfrizzing. It also work to nourish scalp for best strong hairs.
Triglycerides help repair surface of the hair it also covers split ends. As triglycerides
are sticking to the surface of hair it also helps lock moisturizer to give a good shine
and softness to the hair. This packing help environmental damage too.
Shea butter helps scalp health and prevent surface inflammation, which works as asoothing agent.
The formula base is not containing any Mineral oil, Paraben, Phthalate, Silicone andAlcohol.
Keywords: Hair mask, hair conditioner, natural origin ingredient, natural fruit
extracts, triglycerides

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Asian Journal of Management Sciences & Education Vol. 11(1) January-April 2022__________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________INTRODUCTION
There are so many variables which can impact hair in everyday life. Air Pollution, humidity,
not enough care and stress are some of the main cause of hair fall. Some of the elements are
related to vitamin and mineral deficiencies. Perhaps some of the elements are mainly
dependent upon physical state of hairs. Also very much dependent on static charges and thebio-physical conditions of the hair; the environment factor results in breakage of hair fromthe shaft. Sometime it can worsen the hair texture, which impact long term hair roughness
and hair loss. The product which we trial contains triglycerides, Fruit extracts andmoisturizers to treat hair and make up the roughness to give it shine and perfectmanageability. It also help to control frizz and smoothen the hair cuticles to trap all day longmoisture. The overall blend in the formula works in very fastest way to help maintain hair in
2 minutes.
The formula composition also help nourish scalp. Give confidence to the user and prevent
damaging to the hair from the root. The product is also works in leave on and can be used asa cream.
Study Design
The objective of this study was to evaluate and compare in vitro efficacy of hair mask
formulation with respect to sensorial attributes on hair tresses for products.
The test products is thick creamy like texture and smooth with fruity fragrance
Hair Tresses-Total 12 Indian natural black hair tresses.

-

Glued weft Indian-natural hair
Length

20 cm

Width

2.5 cmWeight3.0 gm (approx.)

Product Application-The product application was done in 2 treatment groups.

-

Group I: 0.3 ml of product was applied and allowed to rest on tresses for 2 minutes
and then rinsed off.

-

Group II: 0.3 ml of product was applied and allowed to rest on tresses for 3 minutes
and then rinsed off.

-

The evaluation was carried out under a controlled temperature and relative humidity(temperature:-

20°C to 25°C, hygrometry: 60 ±10%). The lighting is ensured by a ceiling lamp.

-

6 hair tresses for each group were evaluated by each of 10 experts as sensory
evaluator and-Dia-stron meter.

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Asian Journal of Management Sciences & Education Vol. 11(1) January-April 2022__________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________Procedure-Hair tresses from the same bundle were checked for uniformity and quality.

-Total 12 hair tresses (6 per group) were used for evaluation.

-Cleaned with 15 % SLES and dried hair tresses overnight.

-

Baseline sensory analysis was performed.

-

Product application- Product was dispensed onto a wet hair swatch. The procedure forwetting the swatch and the placement of the product on the swatch was kept constant.

-

Product was allowed to rest on tresses for 2 minutes & 3 minutes respectively.

-

Washed off the test product under running tap water.

-

Gently combed individual tresses to remove major tangles.

-

Allowed the tresses to dry overnight.

-

Post application sensory analysis for parameters was assessed.

- 10 experts of panel to evaluate each of 6 hair tresses per group.
Studied Parameters-The evaluation was performed using a scale from 0 to 10.

-

The parameters evaluated were manageability, shine, smoothness, moisture & frizz.

Manageability

Ease(LowofHigh)

Shine

Amount(LowoflightHigh)Smoothness

Amount of or lack of a rough/ harsh feeling along hair swatch*(LowHigh)

MoistureAmount of or lack of a hydration/ dryness feel along hair swatch*(Slightly Very)Frizz

combingreflected

hairoffswatch*hairswatch*

Amount of or lack of a frizz i.e., fuzzy small tight curls along hairswatch* (Low High)*As compared to baseline on visual scaleInterpretation of results-The significant difference in average grade signifies better effect of the product.

Data Analysis & Statistic
The results include:-Raw values for each subject at each examination.

-Differences, in relation to T0 for each subject during the study (Tn – T0).

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Asian Journal of Management Sciences & Education Vol. 11(1) January-April 2022__________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________-Means, medians, maximum, minimum and standard deviations of the raw values andof the differences in relation to T0 obtained by the entire panel.

-Numbers and percentages of subjects presenting an improvement.

Comparison in time for product
The normality of the distributions is checked using Shapiro-Wilk test, threshold at 1%.
The statistical analysis of the evolution of the measured parameters during the study isperformed using the Student test (normality of distributions checked) or with theWilcoxon test (normality of the distributions rejected). The significance threshold isfixed at 5%.
RESULTS
Sensory Evaluation Analysis
The studied parameters are:- Manageability- Shine- Smoothness- Moisture- Frizz
A significant difference in average grade for manageability, shine, smoothness, moisture &frizz shows an effect of the product in terms of improvement in above parameters.
Observed Results for Group I (T+2 minutes after application)
Raw values
The following table presents the means and standard deviation of the raw values of thestudied parameters on hair tresses treated with the test Product A at T0 (initial) and T1 (2minutes after application), as well as the corresponding statistical analysis (Student t test,two-tailed for paired groups at 5%, after the checking the normality of the distributions by a
Shapiro Wilk test at 1%).

Manageability

Shine

Smoothness

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RAW VALUES (GROUP I)
T0T+2 minutes after product application
Mean
4.43 5.87
Standard deviation
0.19 0.30
Significant at 5 % (T0 vs Tn)
Yes
p=2.62E-03
Test
Student t test (two-tailed)Mean
5.30 6.80
Standard deviation
0.22 0.36
Significant at 5 % (T0 vs Tn)
Yesp=<0.001
Test
Student t test (two-tailed)Mean
5.00 6.30
Standard deviation
0.31 0.18
Significant at 5 % (T0 vs Tn)
Yes
p=<0.001
Test
Student t test (two-tailed)
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Asian Journal of Management Sciences & Education Vol. 11(1) January-April 2022__________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________Mean
Standard deviation
Significant at 5 % (T0 vs Tn)
p=TestMeanStandard deviation
Significant at 5 % (T0 vs Tn)
p=TestMoistureFrizz

5.33
0.35

5.50
0.12

6.73
0.15
Yes
2.86E-03
Student t test (two-tailed)
4.67
0.12
Yes
3.64E-04
Student t test (two-tailed)

Yes: Significant difference in favor of the productEvolutions (T1-T0):
The following table presents the means and the standard deviations of the evolutions (T1-T0)of the studied parameters, observed on the hair tresses treated with the test Product A.
EVOLUTION OF THE PARAMETERS(Tn-T0)T+2 minutes after product application- T0
Manageability

MeanStandard deviation

1.43
0.48

Shine

Mean
Standard deviation

1.50
0.39Smoothness

MeanStandard deviation

1.30
0.22

Moisture

MeanStandard deviation

1.40
0.48

Manageability

MeanStandard deviation

-0.83
0.17

Variations (T1-T0)/T0 %The following table summarizes the average percentages of the variation (T1-T0)/T0 of thestudied parameters, observed on the hair tresses treated with the test Product A, calculated
from the average values.
VARIATIONS IN PERCENTAGES*Manageability
Shine
Smoothness
Moisture
Manageability(T+2 minutes after product application- T0)/ T0
32.33%
28.30%
26.00%
26.25%-15.15%*Calculated Based On Mean Value

Analysis- Manageability: Significant increase is noted in this parameter by 32.33%.
- Shine: Significant increase is noted in this by parameter by 28.30%.
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Asian Journal of Management Sciences & Education Vol. 11(1) January-April 2022__________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________-Smoothness: Significant increase is noted in this parameter by 26.00%.
Moisture: Significant increase is noted in this parameter by 26.25%.
Frizz: Significant decrease is noted in this parameter by -15.15%.

Observed Results for Group II (T+3 minutes after application)
Raw values and Statistics
The following table presents the means and standard deviation of the raw values of thestudied parameters on hair tresses treated with the test Product A at T0 (initial) and T1 (3minutes after application), as well as the corresponding statistical analysis (Student t test,
two-tailed for paired groups at 5%, after the checking the normality of the distributions by aShapiro Wilk test at 1%).
RAW VALUES (GROUP II)
T+3 minutes after productT0application

Manageability

Shine

Smoothness

MoistureFrizz

MeanStandard deviation
Significant at 5 % (T0 vsTn)
p=
Test
Mean
Standard deviation
Significant at 5 % (T0 vsTn)
p=
Test
Mean
Standard deviation
Significant at 5 % (T0 vsTn)
p=
Test
Mean
Standard deviation
Significant at 5 % (T0 vsTn)
p=
Test
Mean
Standard deviation
Significant at 5 % (T0 vsTn)
p=
Test

4.43
0.19

5.30
0.22

5.00
0.31

5.33
0.35

5.50
0.12

6.13
0.27
Yes
1.05E-03
Student t test (two-tailed)
7.10
0.35
Yes
<0.001Student t test (two-tailed)
6.90
0.32
Yes
<0.001Student t test (two-tailed)
7.37
0.36
Yes
<0.001Student t test (two-tailed)
3.80
0.36
Yes
1.05E-03
Student t test (two-tailed)

Yes: Significant difference in favor of the product

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Asian Journal of Management Sciences & Education Vol. 11(1) January-April 2022__________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________Evaluations (T1-T0)
The following table presents the means and the standard deviations of the evolutions (T1-T0)of the studied parameters, observed on the hair tresses treated with the test Product
EVOLUTION OF THE
PARAMETERS (Tn-T0)
T+3 minutes after productapplication- T0
MeanStandard deviation
Mean
Standard deviation
Mean
Standard deviation
Mean
Standard deviation
Mean
Standard deviation

Manageability
Shine
Smoothness
Moisture
Manageability

1.70
0.45
1.80
0.22
1.90
0.15
2.03
0.07
-1.70
0.45

Variations (T1-T0)/T0 %:The following table summarizes the average percentages of the variation (T1-T0)/T0 of thestudied parameters, observed on the hair tresses treated with the test Product A, calculated
from the average values.
VARIATIONS IN PERCENTAGES*(T+3 minutes after productapplication-T0) / T0
Manageability

38.35%

Shine

33.96%

Smoothness

38.00%

Moisture

38.13%

Manageability-30.91%*Calculated Based On Mean ValueAnalysis- Manageability: Significant increase is noted in this parameter by 38.35%.
- Shine: Significant increase is noted in this by parameter by 33.96%.
- Smoothness: Significant increase is noted in this parameter by 38.00%.
- Moisture: Significant increase is noted in this parameter by 38.13%.
- Frizz: Significant increase is noted in this parameter by -30.91%.

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Asian Journal of Management Sciences & Education Vol. 11(1) January-April 2022__________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

Graphical Representation

DISSCUSIONSIn the experimental conditions, we have demonstrated following for:
After 2 minutes of application of test product, significant difference observed in the average
grade of all sensorial attributes, which shows effect of product in terms of improvement inhair manageability, shine, smoothness, moisture & frizz by 32.33%, 28.30%, 26.00%,26.25% & -15.15% respectively.
After 3 minutes of application of test, significant difference observed in the average grade ofall sensorial attributes, which shows effect of product in terms of improvement in hairmanageability, shine, smoothness, moisture & frizz by 38.35%, 33.96%, 38.00%, 38.13% & 30.91% respectively.
OBSERVATION AND CONCLUSION
1.

Hair swatches treated with product is showing significance improvement in hair after useof hair mask/conditioner. Hair become more Manageable, Shines, Smooth and no frizz.
Even if leave 2 minutes after application. This is very quick and instant solution.

2.

Similarly at 3 minutes leave on after application is showing very good performance forhair Manageability, Shine, Smoothness and Frizzing.

3.

The formula doesn’t contain nasties like Mineral oil, Paraben, Silicone, Phthalate andAlcohol.

4.

The product is worked as damage control for hair from environmental factors.

5.

The product is full of goodness of natural origin ingredients. Having infused with naturalfruit extracts.

ACKNOWLEDGEMENT
The researchers thank to Mr. Kirshna Kumar Chutani, CEO and Mr. Subba Rao – Head ofoperation for their constant encouragement and support for the study.

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Asian Journal of Management Sciences & Education Vol. 11(1) January-April 2022__________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________REFERENCES[1].

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