2021-2022

https://www.infront.kyoto-u.ac.jp/?lang=en

A Message from the Director
Recent many discoveries in molecular biology have led toaccelerating progression of medicine and biology. In
particular, since the completion of decoding the humangenome in 2003, it is now possible to obtain genome

Research for the Frontier

information of many organisms as well as genome
information of individuals and ancient people. We enter an ew p ha se of d eve l o p m e nt to c l a r i f y th e fact-ba se dmeaning of this information.
This institute is based on the Institute for Frontier Medical
Science, generated the establishment of human embryonicstem cells (ES cells), discovery of iPS cells and regulatory T
cells, and the Institute for Virus Research, led the dawn ofmolecular biology in Japan and discovered human T cellleukemia virus. From merging these institutes "Institute for
Frontier Life and Medical Sciences" started in October 2016.
After the organizational integration, "Department of Virus
Research", "Depar tment of Regeneration Science andEngineering", and "Department of Biosystems Science"c olla b orati ve l y p e r for m v ir us re se a rc h, re g e ne rati ve
medicine research, and Biosystem research for humans. To
complement these activities, we have "Research Center for
Infectious Diseases", "Center for Animal Experiments", and"Center for Human ES Cell Research" as affiliated facilities.
M o d e r n m e di c a l li fe sci e nc e ha s inc or p o rate d nove ltechnologies by fusing with scientific outcomes from otherfields. This research organization will continue after 2022.
Our institute has been appointed as Joint Usage/ResearchCenter for Transdisciplinar y Collaboration on T issue
Engineering and Regenerative Medicine and as Joint
Usage/Research Center for Fusion of Advanced
Technologies and Innovative Approaches to Viral Infectionsand Life Science. In addition, we have many achievements
to further develop the basic functions and to flourish as abiomedical research hub.
It is ex tremely impor tant to make use of science andtechnology outcomes that explores life widely and deeply
for human society. As of 2021, there is still struggle forcontrol COVID-19. We will continue our educational andresearch activities in order to utilize novel knowledge forhuman societ y. We are striving ever y day to fulfill our
mission as a research organization and scientists that
contributes to humanity. We would appreciate your support.
May, 2021

Yoshio Koyanagi,

MD., Ph.D.

Director and Professor
Institute for Frontier Life and Medical Sciences

History

Data
1941

Tuberculosis
Research Institute

Institute for Virus Research1956

5 Departments (6 Departments& 1 University Hospital, eventually)

2 Departments

1957

1967

4 Departments

1958

1980
Research Center for Medical
Polymers and Biomaterials

5 Departments

1959

5 Departments& 1 Research Center

1962

6 Departments& 1 Research Center

1968

3 Departments

8 Departments& 1 Research Center

1978

8 Departments& 2 Research Centers

1990

1988

Chest Disease
Research Institute

4 Divisions (after reorganization),
1 Research Center & 1 University Hospital

1998

Institute for Frontier
Medical Sciences

4 Departments (after reorganization)& 2 Research Centers

2005

6 Departments & 1 University Hospital(7 Departments, 1 Research Center &1 University Hospital, eventually)

1990
Research Center for
Biomedical Engineering

7 Departments& 1 Research Center

1974

Chest Disease
Research Institute

5 Fields (after reorganization) & 1 Research Center(5 Fields & 2 Research Centers, eventually)

4 Departments& 3 Research Centers

2016

Faculty and Staff (As of May 1st, 2021)
3

Program-SpecificResearcher

13

Re-employed
staff

1

2

Technical Staff

3

Program-Specific
Assoc.Prof.
Program-SpecificSenior Lecturer
Program-Specific
Assist. Prof.

9

Researcher(Part-Time)

31

Assist.Prof.

28

SUBTOTAL

12Administrative
Staff

4

Other

73

SUBTOTAL

63（6）

TOTAL

75（6）

Professor

17（5）Assoc.Prof.

15（1）Senior Lecturer

Research Fellows and Research Students (As of May 1st, 2021)

Graduate Students (As of May 1st, 2021)
Graduate Schoolof Medicine

15

Graduate School of Human andEnvironmental Studies

7

Specialresearch student

4

JSPS*

1

Graduate Schoolof Science

10

Graduate Schoolof Biostudies

43

Research student

5

Contracted
Researcher

727

Graduate School of
Pharmaceutical Sciences

1

Private Sector
Researcher

11

Graduate Schoolof Engineering

11

Research Fellow

113

TOTAL

Other subsidies

Donations 105,614,685

17,400,000*The Japan Society for the Promotion of Science

International exchange (As of May 1st, 2021)

寄附金[China]China Medical University
China Rehabilitation Research Center

Personnel Expenses
その他補助金(University grants)

1,344,016,847

849,672,380

産学連携等研究費Academic

TOTAL

科学研究費

3,780,227,115

物件費

Cost of equipment
人件費(University grants)

Grants‐in‐aid
for Scientific
Research

29

TOTAL

Financial data in academic year 2020 [Unit: JPY]

（established on Oct. 1st）

3 Departments (28 Laboratories & 6 Laboratories of visiting professors)& 2 Research Centers (3 Laboratories)

131

TOTAL

*Numbers in parenthesis indicate visiting professors

Research funds

Institute for Frontier Life and Medical Sciences

7

exchangememoranda[Taiwan]College of Oral Medicine, Taipei Medical
University[Germany]
Bonn Institutes of Immunosciences andInfection, Medical Faculty, University of Bonn

1,048,316,034

415,207,169

Joint Usage / Research Center Initiatives
2020

3 Departments (28 Laboratories & 6 Laboratories of visiting professors)& 3 Research Centers (5 Laboratories)

The original cover picture
The motif of the cover picture is from wall decorative paintings“Stoclefries”, one of the masterpieces of Gustav Klimt (1862-1918)
in the late Austrian Empire.
Klimt’s work is described as giving the impression of the “chainof life and death” as well as the “permanency of life” because
there is always scent of death in his gorgeously colored paintings.
On this motif, we overlaid a “formula”, a common language ofscience, to express how a basic unit of life such as a nucleic acidmolecule, a virus, a cell, an organ or a concrete life existence(consisted of the basic units of life) leads a dynamic life. Thisshows our direction to fulfill our mission to research into “variously
structured cell society” of life in order to reveal the whole
structure of strategy for life to exist.

3

The following two joint usage/research centers have been designated by the Ministry of Education, Culture, Sports, Science and Technology within
our institute, offering our resources and research techniques to research communities in Japan and overseas through joint research initiatives.

Joint Usage/Research Center for Fusion ofAdvanced Technologies and Innovative
Approaches to Viral Infections and Life Science

Joint Usage/Research Center forTransdisciplinary Collaboration on Tissue
Engineering and Regenerative Medicine

P3 level infection experiments using mice and monkeys are indispensable
to understanding human infectious diseases and developing new therapeutic strategies for applications in clinical settings. These experiments
require animal-raising facilities as well as supervision, support and education by academic and technical staff with sufficient expertise. The instituteis equipped with a large-scale facility for P3 infection experiments involving mice and monkeys. As well as virus research at the gene and cell levels
using state-of-the-art research techniques, we use this facility to conduct
in vivo infection experiments. Our accumulated research techniques are
distributed widely to research communities through joint usage/research.
Major research outcomes in FY2020 included the development of anantiviral screening method for Lassa fever virus and the identification
method of unknown virus-like sequences in the human genome, comprehensive gene expression analysis at the single cell level of HIV-1-infected
cells, and the identification of viral protein of SARS-CoV-2 that suppresses
interferon response, the identification of molecules involved in quality
control in the process of synthesizing membrane proteins. These joint
research efforts were published in high-impact international scientificjournals.

Based on our expertise and technology in regenerative medicine, we
promote a wide range of cutting-edge joint research projects and assist
the education and training of researchers actively engaged in regenerative
medicine.
Our main research includes fundamental research to understand biological mechanisms, regulations and materials and develop related technologies and applied research to develop treatments and create regenerative
tissues or organs for clinical applications.
In FY2020, we conducted 20 projects including three international joint
research projects and actively collaborated with researchers including
many junior scientists and postgraduate students. We also promoted joint
usage of our animal laboratories, with the total number of users reaching
2,829. We actively organize lectures for the general public and high school
students, supply various research resources including human ES cells,
and promote joint usage of our animal laboratories.

Number of approved joint research projects (FY2021)
En t w u r f fu r d e n Wa n d fr i e s i m Pa la isStoclet in Brussel, Goldener Ritter - 1909

Number of approved joint research projects (FY2021)
1. Interdisciplinary research5

1. SARS-CoV-2 Research

10

2. Exploratory research20

2. Virus Research

8

Total

25

3. Life Science Research

10

Total

28

4

Department of Virus Researchorganization
Research Departments

Committee
for Programmingand Management

Department of Virus ResearchLab. of Medical Virology …………………………………………… 6
Vice Director

Director

Lab. of RNA Viruses ………………………………………………… 7
Lab. of Ultrastructural Virology …………………………………… 8Lab. of Tumor Viruses ……………………………………………… 9

Vice Director

10Lab. of Cell Regulation ………………………………………………
Lab. of Immune Regulation ……………………………………… 11
Lab. of Bioresponse Regulation ※ ……………………………… 12

Faculty Council

Lab. of Viral Immunology ※ ……………………………………… 14

Department of Regeneration Science and Engineering
Lab. of Molecular and Cellular Biology ………………………… 15

Steering Committee on"Joint Usage/ Research Center
Program for Fusion of AdvancedTechnologies
and Innovative Approaches to Viral Infectionsand Life Science"Lab. of Biomaterials ……………………………………………… 16
Lab. of Immunology ……………………………………………… 17
Lab. of Tissue Regeneration …………………………………… 18
Lab. of Developmental Epigenome …………………………… 19
Lab. of Integrative Biological Science ………………………… 20
Lab. of Experimental Immunology ※Steering Committee on"Joint Usage/Research Center
Program for Transdisciplinary
Collaboration on Tissue Engineeringand Regenerative Medicine"Lab. of Material Biophysics ※………………………… 21………………………………… 22

Department of Biosystems Science
Lab. of Biomechanics …………………………………………… 23
Lab. of Developmental Systems ……………………………… 24
Lab. of Systems Virology ………………………………………… 25
Lab. of Growth Regulation System …………………………… 26
Lab. of RNA System ……………………………………………… 27
Lab. of Biological Membrane System ………………………… 28
Lab. of Tissue Homeostasis …………………………………… 29
Lab. of Mathematical Biology …………………………………… 30
Lab. of Stem Cell Genetics ……………………………………… 31
Lab. of Cell Fate Dynamics and Therapeutics ……………… 32

Lab. of Medical Virology
Infectious diseases still have remained a fatal

200,000 deaths were reported in 2019. MeV

threat to children, worldwide. To solve theusually causes acute measles, but in rare

problem, we have been studying on pediatricinstances induces fatal and intractablev i r o l o g y. I n p a r t i c u l a r, w e f o c u s o n t h eneurological diseases. MuV causes epidemic

mechanisms of viral entry into cells and theparotitis, meningitis, encephalitis and deafness.

inhibition of entry by compounds, peptides,

Large outbreak of mumps occurs once every

glycans, and antibodies, using a combination off o u r to f i v e y e a r s i n J a p a n. C u r r e n t l y n ovirological and structural biologicallicensed therapeutic agents are available forapproaches. Our major goals are theboth viruses, and the mechanisms that cause

e l u c i d ati o n of v i ra l path o g e n e s i s a n d th e

CNS diseases remain unknown. Therefore, wedevelopment of preventive and therapeutic

are currently working on research to solve

methods for viral diseases. Our laboratory wasthese problems.

newly joined in this institute in September 2020,

Our laborator y has been also studying theand started to research on paramyxovirusesd eve l o p m e nt of n ew d r u g s fo r i nfe c ti o u s

and coronaviruses.

diseases caused by SARS-CoV-2, Ebola andMeasles virus (MeV) and mumps virus (MuV),

Marburg viruses, and Nipah virus, for which

members of the family Paramyxoviridae, arebiosafety level 3 or 4 is required. One of ourimportant human pathogens causing

goals is to develop vaccines, therapeutics, andrespiratory and neural infections. Globally, MeV

new methods of immunoanalysis using

has been causing outbreaks recently and overstructural information.

Prof. Takao Hashiguchi
E-Mail:takaoh@infront.kyoto-u.ac.jp
Assist. Prof. Tateki Suzuki

E-Mail:suzuki.tateki.7y@kyoto-u.ac.jp

Lab. of. Deconstruction of Stem Cells ………………………… 33
Lab. of Regulatory Information ※ ……………………………… 34
Attached Facilities
Unveiling of Nameplate “Institute for Frontier Life andMedical Sciences Kyoto University” (October 3rd, 2016)

Research Center for Infectious Diseases
Lab. of Primate Model …………………………………………… 35
Lab. of Infectious Disease Model ……………………………… 36
Lab. of Virus-Host Coevolution ………………………………… 37

Center for Animal Experiments …………………………………… 38
Center for Human ES Cell Research …………………………… 41
Division of Clinical Basis for ES Cell Research ……………… 42
Division of Basic Technology Development for ES Cell Research

Department of Cell Biology
Division of Technical Support
Establishment Ceremony of Institute for Frontier Life andMedical Sciences Kyoto University (December 21st, 2016)

5

Administration Office※Visiting

Enveloped viruses possess glycoproteins that interact with receptors and other host factors to invade target
cells via sequential membrane fusion and exert pathogenicity against humans. On the other hand, viral
glycoproteins are also critical antigens in human immune responses and are one of the targets of antiviraldrugs. Therefore, they are important not only in viral pathogenicity but also in the development of vaccines andnew drugs. In our laboratory, we aim to elucidate the detailed mechanisms of infection and its inhibition by
integrating virology with structural biology and drug discovery science.
Lab URL

https://medvirology.infront.kyoto-u.ac.jp/

6Department of Virus ResearchLab. of RNA Viruses
Prof. Keizo Tomonaga
E-Mail:tomonaga@infront.kyoto-u.ac.jp
Assist. Prof. Akiko Makino

E-Mail:amakino@infront.kyoto-u.ac.jp

Department of Virus ResearchLab. of Ultrastructural Virology

All viruses rely on the cellular machiner y to

nucleus. The understanding the biological andO u r l a b o r a to r y h a s b e e n s t u d y i n g n e g a -only fundamental, but also practical research.

complete their replication cycles. Therefore, theevolutional significances of the endogenous

tive-strand RNA viruses such as influenza virus

In addition, our laboratory is skilled at imaging

study of viruses can provide fundamental

bornavirus-like elements (EBLs) found in theand Ebola virus, which are pathogenic for

analyses by using microscopes. In addition toknowledge and understanding not only of viralgenomes of many mammalian species is one ofhumans and animals. Espe cially, we have

conventional virological, molecular biological,

pathogenesis and host responses but also of

the main focuses of our laboratory. Further-focused on: 1. The packaging mechanisms ofand cellular biological techniques, we employ

cellular function. The researches carried out in

more, we are analyzing emerging bornaviruses,

influenza virus eight-segmented genome, 2.

microscopic analyses such as transmission

Assist. Prof.

this laboratory are focused on negative strand

which include avian bornaviruses as well as a

Mechanisms of influenza virus genome

electron microscopy, cryoelectron microscopy,

E-Mail:RNA viruses replicating in the cell nucleus,

squirrel bornavirus that may be highly patho-transcription and replication, 3. Mechanisms ofand high-speed atomic force microscopy toespecially bornaviruses. All our projects aim to

genic to humans. We also aim to develop a

Ebola virus helical nucleocapsid formation, 4.

understand virus replication mechanisms fromunderstand the fundamental mechanisms ofnovel RNA virus vector using bornavirus, which

Generation of neutralizing monoclonal antibod-

an ultrastructural point of view. We would like tothe replication, pathogenesis and evolution of

can stably express foreign genes, including

ies inhibiting influenza virus and Lassa virus

contribute to the progress of virus research as

bornaviruses. In current researches, we arefunctional small RNAs, and be applied for gene

replication, 5. Development of antiviral drugs bywell as the control of infectious virus diseases

investigating the replication and persistenttherapy of stem cells, such as iPS cells.

drug repositioning, and 6. The structure of influ-

through our research.

mechanism of the bornaviruses in the cellenza virus mRNAs. So our interests cover not

Prof. Takeshi Noda
E-Mail:t-noda@infront.kyoto-u.ac.jp

Masahiro Nakano
nakanom@infront.kyoto-u.ac.jp
Assist. Prof.

Yukiko Muramoto
E-Mail:muramo@infront.kyoto-u.ac.jp
Program-Specific Assist. Prof.

Yukihiko Sugita
E-Mail:sugita.yukihiko.8w@kyoto-u.ac.jp

In Lab. of RNA viruses, we are working on several projects regarding replication/pathogensis
In Laboratory of RNA viruses, we are working on several projects regarding replication/
pathogenesis of bornaviruses, endogenous bornavirus and development of novel RNA virus
vectors using bornavirus.

Lab URL

7

https://t.rnavirus.virus.kyoto-u.ac.jp/

Figure 1 Transmission electron microscopic image offilamentous Ebola virus particles budding
from infected cells.

Lab URL

Figure 2 Three dimensional model of an influenza virus
particle reconstructed by electron tomography. Eight RNPs arranged in a characteristic"1+7" pattern are present within the virion.

https://www.facebook.com/NodaLab/

8

Department of Virus Research

Lab URL

Department of Virus Researchhttps://www.infront.kyoto-u.ac.jp/ex_ivr/Lab/sakai2012/Home2.html

Assoc. Prof.

Hiroyuki Sakai
E-Mail:hsakai@infront.kyoto-u.ac.jp
Prof. Masahiko Sugita
E-Mail:msugita@infront.kyoto-u.ac.jp
Assist. Prof.

Daisuke Morita
E-Mail:dmorita@infront.kyoto-u.ac.jp
Assist. Prof.

Tatsuaki Mizutani
E-Mail:mizutani@infront.kyoto-u.ac.jp

Lab URL

https://www2.infront.kyoto-u.ac.jp/HCV/

Assoc. Prof.

Makoto Hijikata
E-Mail:mhijikat@infront.kyoto-u.ac.jp

Lab URL

https://www2.infront.kyoto-u.ac.jp/SugitaLab/SugitaLab.html

Department of Virus Research

Department of Virus ResearchLab. of Bioresponse
Regulation (Visiting)Lab. of Immune Regulation
Prof. Koichi Ikuta
E-Mail:ikuta.koichi.6c@kyoto-u.ac.jp

The immune system has acquired sophisticatedIL-7. We are now carr ying out the following

control mechanisms as a result of evolution at

projects: (1) function of IL-7 receptor in differen-

the front line of the battles between hosts andtiation, maturation and response of immune

pathogenic microorganisms. Cytokines are acells; (2) regulation of IL-7 receptor expression

group of proteins important for controlling theduring lymphocyte development and immune

Assist. Prof.

immune system. Interleukin-7 (IL-7), one of the

response; (3) circadian control of dynamics andE-Mail:cytokines, plays important roles in differentia-function of lymphoid cells by steroid hormones

tion, maintenance and response of lympho-

and sex difference in the immune system; andcytes and innate lymphoid cells, and is essen-(4) visualization and local function of cy to-tial for organogenesis of lymphoid organs. We

kine-producing cells, in relation with tumor

are pursuing research on development andimmunity.

Takahiro Hara
hara.takahiro.8r@kyoto-u.ac.jp
Assist. Prof.

Guangwei Cui
E-Mail:cui@infront.kyoto-u.ac.jp

Even in the present day with medical

antiviral drugs, we study influenza

progress, once a pandemic due to anvirus infection in a macaque model in

infectious disease occurs, it cannot be

the BSL-3 nonhuman primate facility

controlled, resulting in huge human andat this institute. We have also been

economic losses. Pandemic influenza

analyzing SARS-CoV-2, and foundviruses appear occasionally. The mostthat SARS-CoV-2 isolates replicate

recent influenza pandemic occurred inefficiently in the lungs of Syrian ham-2009 when pandemic (H1N1) 2009 influ-

sters and cause severe pathologicalenza arose and spread quickly world-

lesions in the lungs of these animals

wide. In 2020, a pandemic caused bysimilar to commonly reported imaging

SARS-CoV-2 began and continues tofeatures of COVID-19 patients withcause enormous damage around thepneumonia. Syrian hamsters are aworld. We are studying how to control

usef ul small a nimal mode l for thethese pandemic viruses.

evaluation of vaccines, immunothera-

To better understand pathogenesis andpies, and antiviral drugs.

to improve the efficacy assessment of

Pre

Day 7

Prof. Yoshihiro Kawaoka
E-Mail:kawaoka@ims.u-tokyo.ac.jp

Day 16

response of the immune system, focusing on

Assist. Prof.

Keiko Takemoto
E-Mail:takemoto.keiko.6r@kyoto-u.ac.jp

Figure 1. Images of the lungs of a SARS-CoV-2-infected hamster obtained by using microCT.
The infected animal developed severe pneumonia (white) and a pneumomediastinum(red) at 7 days post-infection. By 16 days post-infection, the pneumonia had resolved
and the pneumomediastinum had disappeared. The trachea and bronchi are coloredin blue.

Figure 1 Immunoenhancing effects of glucocorticoids
Glucocorticoids drive diurnal oscillations in T cell distribution and responses by
inducing IL-7R and CXCR4.

Figure 2 IL-15-expressing cells in lymph nodes

Figure 2. Analysis of influenza viruses in a macaque model at the BSL-3 nonhumanprimate facility at this institute.

Immunohistochemistr y of lymph nodes
from IL-15-CFP knock-in mice. IL-15/CFP(red), fibroblastic reticular cells (green),
dendritic cells (blue). IL-15 production isdetected in stromal cells and blood vascular endothelial cells. B, B cell-rich follicles;
T, T-cell zone; and M, medulla.

Lab URL

11

https://www2.infront.kyoto-u.ac.jp/Ikuta-Lab/12

Department of Virus Research

Department of Virus ResearchLab. of Bioresponse
Regulation (Visiting)

Hepatitis viruses constitute a serious public health problem
affecting more than 300 million people worldwide, which function as exogenous ligands inducing an inbalance of physiological condition to restricted hosts. We study these viruses toestablish an experimental model evaluating infection and its
cellular responses, to analyze spatio-temporal virus dynamics
and its principle for survival during interaction with environment,
and to develop a strategy for controlling these virus infections.
We are also developing antiviral strategy against the novel
coronavirus. Especially, our chemical genetics approach usingchemical probes that manipulate virus infection enables to

Assoc. Prof.

Koichi Watashi

progress basic virology in concert with drug development.

E-Mail:kwatashi@nih.go.jp

Figure 1 Chemical probes manipulating the dynamics ofhepatitis B virus cell entry

TopicsLab. of Viral Immunology (Visiting)
Human T-cell leukaemia virus type 1 (HTLV-1) isin the UK and overseas, especially in Japan.

widespread in the tropics and subtropics. Ninety

Continuing our collaboration with colleagues inpercent of people infected with this virus are

Japan, we have discovered (Satou et al 2016:

unaware of the infection and remain healthy, but

Proc. Nat. Acad. Sci. USA; Melamed, Yaguchi et

5% develop a leukaemia or lymphoma, known

al 2018: eLife) that HTLV-1 alters the structureas ATL, and up to a further 5% develop a chron-

and transcription of host chromatin. This highly

ic inflammatory disease of the nervous system

unexpected observation raises new hypotheses

known as HAM/TSP, which results in paralysis ofabout the pathogenesis of the leukaemia associ-the legs. HTLV-1 is the main cause of adultated with HTLV-1 infection, and about the evolu-

leukaemia in southern Japan.

tion of transposable elements in the mammalianProf. Charles Bangham
E-Mail:c.bangham@imperial.ac.uk

genome. In addition, we found (Kirk et al 2016:
We aim to answer the questions:

Nature Microbiology, doi: 10.1038/NMICROBI-• why do some HTLV-1-infected people develop

OL.2016.212) that HTLV-1 and other exogenous

the se serious disease s, while the majorit y

retroviruses integrate into a shared, non-palin-remain healthy? anddromic DNA sequence motif, unlike what has• how does HTLV-1 persist lifelong in the individ-

been believed for the last 25 years.

ual, despite a strong immune response?

In 2020 we established an Institute of Infection in
Imperial College. The purpose of the Institute is

In the Imperial College laboratory we study theto drive multidisciplinary research in infectious

immunology and virology of HTLV-1 infection,diseases and the agents that cause them, byLab. of Systems Virology

using a wide range of molecular, cellular andbringing together researchers in biology andIn Japan, COVID-19 national task force gathered researchers to develop strategy to control themathematical techniques. We have longstand-medicine with those in physical sciences, engi-

outbreak based on scientiﬁc evidence. The team was named Cluster Response Team. I haveing and valuable collaborations with colleagues

neering and mathematics.

been working for the team since the beginning of the outbreak as an expert of epidemiology
and virology.

Discovery of the virological synapse (VS):triggered, directional transfer of HTLV-1 from cell to cell

The team has conducted various activities from data collection and analysis to mathematicaland statistical modeling; risk management for border control, community transmission, and30 minutes

Gag protein complexes(red) polarize to thecell-cell contact area

nosocomial outbreak; and countering stigma. It was exhausting but exiting to work for society
using skills I have learned and acquired through my past research. Furthermore, the experience
there can motivate me and bring in new idea for further research in the future, which will hopefully in turn contribute to public health.
Program-Specific Assoc. Prof.

Yuki Furuse- which contains
organized adhesion
domains (green)

120 minutes

Gag is then transferredwith the HTLV-1 genome
to the target cell

Igakura et al 2003: Science 299, 1713-6

13

Dendritic cells (blue) can also be
ef ficiently infected by contact
w i th a n H T LV-1-i nfe c te d c e l l(green)14

Department of Regeneration Science and Engineering

Department of Regeneration Science and Engineering

Lab. of Molecular
and Cellular Biology

Our research focuses on the following three projects: Analysis of thequality control mechanism of proteins and the molecules such as
chaperones and lectins that are involved in the mechanism (Hosokawa G); Analyses of transition stage from the formation of pre-initiation
complex to elongation using RNA aptamer (HIrayoshi G); Analysis of
illegitimate V(D)J recombination within T cell receptor β chain geneduring normal T cell development in relation to tumorigenicity(Fujimoto G).
In Hosokawa G, we study on the quality control mechanism ofproteins and on the molecules such as chaperones and lectins thatare involved in this system. Protein misfolding occures when cells are
exposed to various stresses, or when mutations occur in the genesthat endcode proteins. We are also analyzing the protein degradation
mechanism named ERAD (endoplasmic reticulum-associated degra-

Assoc. Prof.

Nobuko Hosokawa

dation), and the intracellular transport of proteins.

E-Mail:nobukoh@infront.kyoto-u.ac.jp

Lab. of Biomaterials
The main objective of our depar tment is tofrom biodegradable and non-biodegradable

proceed the research and development ofmaterials. Our goal is not only to carr y out

methods, procedures, and technologies appli-

researches of tissue regenerative therapy

cable to basic research of biology and medi-(tissue engineering, cell transplantation, cellcine, and medicines (therapy, diagnosis, andresearch, and drug discovery), drug delivery

prophyla xis) from the viewpoint of material

system (DDS), biomedical engineering, gene

Assist. Prof.

sciences. The biomedical materials (biomateri-engineering technology, and stem cell technol-

Mitsuru Andoals) to use in the body and to contact biologicalogy, but also put the research results to clinical

E-Mail:ando@infront.kyoto-u.ac.jp

substances are being designed and createdand practical uses.

Prof. Yasuhiko Tabata
E-Mail:yasuhiko@infront.kyoto-u.ac.jp

Sr.Lect. Kazunori Hirayoshi
E-Mail:ippou@infront.kyoto-u.ac.jp
Assist. Prof.

Shinji Fujimoto
E-Mail:fujimoto@infront.kyoto-u.ac.jp
Chaperone protein complex in the endoplasmic reticulum (ER)
Newly snthesized proteins in the ER obtain their native conformations by the assistance of ER
chaperon proteins. Some chaperone proteins make a complex to assist protein folding and to
inhibit protein aggregation.

Ubiquitin-ligase complex in the endoplasmic reticulum (ER) membrane
Proteins that have misfold in the ER are degraded by the cytoplamic proteasome, a mechanism
named ERAD. The ubiquitin-ligase complex in the ER membrane regulates ERAD. Chaperone
proteins and lectins associate with this complex from the lumenal side.

Technologies developed in this laborator y. (A)Tissue engineering is the research anddevelopment of biomaterial technologies to realize the regenerative therapy by making use ofcell-based natural healing potential. Biomaterials can enhance the cell-based potential toachieve the regeneration and repairing of tissues. (B)Drug delivery system is technologies andmethodologies to maximize the action of drugs (substances with a certain biological activity
and function) by the combination with biomaterials. Drugs include therapeutic, diagnostic, andpreventing drugs or cosmetics. (C)Reverse transfection enables genes to safely internalize intoweak cells of mesenchymal stem cells (MSC) and achieve the prolonged gene expression.
(D)Behavior of stem cells is modified by the stiffness, surface topology, and physicochemical
properties of materials (hydrophilicity and charge etc.) and the extent of protein immobilized tomaterials. The objective of stem cells engineering is to create materials which mimic the cell
environment in the body for cell research and drug discovery.
Lab URL

15

https://www2.infront.kyoto-u.ac.jp/te02/index_en.html

16Department of Regeneration Science and Engineering

Lab. of Immunology
Prof. Hiroshi Kawamoto
E-Mail:kawamoto@infront.kyoto-u.ac.jp

Assoc. Prof.

Masaki Miyazaki
E-Mail:mmiyazaki@infront.kyoto-u.ac.jp
Assist. Prof.

Kyoko Masuda
E-Mail:kyoko.masuda@infront.kyoto-u.ac.jp
Program-Specific Assist. Prof.

Junji Uehori
E-Mail:uehori@infront.kyoto-u.ac.jp
Program-Specific Assist. Prof.

Yuka Kobayashi

Department of Regeneration Science and Engineering

Laboratory of Tissue Regeneration

The major aim of our laboratory is to elucidate

CTLs from patients in ex vivo culture. To solve

the molecular mechanisms that regulate cell

this issue, we have proposed a strategy to use

the pathology of disorders in the musculoskele-

disease-specific iPS cells

fate decisions in the process of lineage restric-

iPSC technology for cloning and expansion oftal system at the molecular level and to develop

One of the advantages of iPS cells is that ittion from multipotent hematopoietic stem cells

tumor antigen specific CTLs; iPSCs produced

new therapeutic modalities by understanding

can be established from any individuals. We

to unipotent progenitors. Among various eventsfrom T cells (T-iPSCs) should inherit rearranged

the physiological growth, differentiation, andestablished iPS cells from patients withoccurring during hematopoiesis, we are mainly

TCR genes, and thus all regenerated T cells

transformation of mesenchymal cells. Following

intractable hereditar y bone and cartilage

focusing on the process towards the produc-from T-iPSCs should express the same TCR.

projects are currently undertaken.

diseases, and using newly developed differ-tion of T cells. We have recently clarified theBased on this idea, we have succeeded in

1. Researches on mesenchymal stem cells

entiation methods, we are performing themechanisms for the maintenance of T cellregenerating MART1-specific CTLs from a

Mesenchymal stem cells (MSC), which exist

disease modeling and drug discover y forlineage (Figure 1).

melanoma patient (Vizcardo et al, Cell Stemin bone marrow stromal tissues, have abone diseases such as fibrodysplasia ossifi-

As another project, we have been developing

Cell, 2013). Recently we have developed apotential to differentiate to cells of various

cans progressiva and osteogenesis imper-a n a p p ro a c h a i m i n g to a p p l y o u r c u l t u r emethod by which CTLs are regenerated fromtypes in mesenchymal tissues. Many funda-

fecta, and grow th plate disease such as

method in clinical settings. Whereas cytotoxic T

iPS cells transduced with exogenous TLR genemental features of MSCs, however, are still

lymphocytes (CTLs) represent the most promis-( TCR-iPSCs) (Fi gure 2). T his n ew m etho dunknown, which are crucial for the develop-

ing therapeutic avenue in cancer immunothera-provides a convincing rationale for application

ment of regeneration therapy using MSC asmas using pluripotent stem cells

py, adaptive transfer of antigen-specific CTLsof this strategy in clinical settings.

the evidence based medicine. We have

Sarcomas are malignant tumors developed

analy zed the grow th and dif ferentiation

in mesenchymal tissues and consisted ofpotential of primary human MSCs.

tumors with a variety of clinical and patho-

has faced difficulty in efficient expansion of

E-Mail:The objectives of our laboratory are to disclose

2. Researches on mesenchymal tissues using

yukacoba@infront.kyoto-u.ac.jp
Program-Specific Assist. Prof.

induced pluripotent stem (iPS) cells

Seiji Nagano

1 ) Investigation for the differentiation process of

E-Mail:s.nagano@infront.kyoto-u.ac.jp
Figure1
Conversion of T cells to B cells by inactivation ofpolycomb-mediated epigenetic suppression
In T cell-specific Ring1A /B deficient mice, T cell
development was severely blocked at an immatures t a g e. We fo u n d th at th e s e d eve l o p m e n t a l l yarrested T cell precursors gave rise to functional B
cells upon transfer to immunodeficient mice (Ikawa
et al, Genes & Development, 2016).

Inter-Organ Communication
Research Team
Program-Specific.Assoc. Prof.

Shinpei Kawaoka
E-Mail:17

E-Mail:togjun@infront.kyoto-u.ac.jp

Assist. Prof.

Yonghui Jin
E-Mail:jinyh@infront.kyoto-u.ac.jp

multiple epiphyseal dysplasia.

logical features. Using iPS cells with drug-inducible driver mutations of each t ype ofsarcoma, we analyze the effect of mutations
in different stages of differentiation. ThisWe are establishing robust and ef ficientapproach may help to explain the heteroge-

differentiation method of bone and cartilage

neity of tumors and also provide information

cells from iPS cells, and investigate thefor personalized medicine.

prcise molecular mechanisms of differentiation.

Figure1
Bone-like nodules formed by GFP-labelled human
iPS cells. The surface of nodules is covered byosteoblasts in a sheet-like structure and osteocyte
with dendritic process migrate into the inside ofnodules.

Figure2
Regenerated killer T cells showed efficacy in a PDX
solid tumor model
iPS cells obtained from CiRA were transduced with
WT1 antigen-specific T cell receptor that had beenclinically tested, and killer T cells were regenerated
from the iPS cells. The regenerated killer T cells weretransferred to the PDX (patient derived xenograft)model mouse of renal cell carcinoma in which both of
WT1 positive and negative tumor tissues had beentransplanted, resulting in the suppression of thegrowth of solely the WT1 positive tumor.

http://kawamoto.frontier.kyoto-u.ac.jp

Prof. Junya Toguchida3 ) Investigation for the cell-of-origin in sarco-

bone and cartilage cells using iPS cells

kawaokashinpei@infront.kyoto-u.ac.jp

Lab URL

2 ) Disease modeling and drug discovery using

Figure2
Safranin O staining of cartilage pellets induced
from human iPSCs via osteochondro-progenitor
cells derived from somites. Hyper trophic
morphology was observed in peripheral lesions at
day 28, a nd most c e lls showe d hy pe r tophic
chondrocyte-like features at day 56.

Lab URL

https://www2.infront.kyoto-u.ac.jp/ca02/index-j.htm

18Department of Regeneration Science and Engineering

Department of Regeneration Science and Engineering

Lab. of Developmental
Epigenome

Regenerative medicine and aging are closely linked subjects.
Stem cell functions in repair and replacement of old tissues
with young tis su e s. Induc e d plur ipote nt ste m ( iPS) c e ll
generated through transformation of somatic cell by forced
expression of reprogramming factors is expected to contributeto regenerative medicine. Anti-aging factors, which function inmaintaining to keep body young, could be related to stem cell.
Reprogramming and anti-aging sharing rejuvenation as a goal

Lab URL

https://www2.infront.kyoto-u.ac.jp/es03/index.html

are regulated by the molecular mechanism of epigenome.

Lab. of Integrative Biological Science

Assoc. Prof.

Takashi Tada
E-Mail:ttada@infront.kyoto-u.ac.jp

Reprogramming of iRS (intermediately reprogrammed stem) cell to iPS cell

Mammalian sperm undergo multiple maturation

found that sperm undergoing GPI-AP release

steps after leaving testis to be competent for

associated with reorganization of lipid raft andfertilization. Serial important changes occur inacrosome reaction acquire fer tilizationt h e f e m a l e r e p r o d u c t i v e t r a c t o n s p e r m,

p o te n t i a l. I n te r m s o f i d e n t i f y i n g f a c to r s

although the molecular mechanisms underlying

triggering these processes in vivo, we foundthese processes remain unclear. In our early

Lipocaline2 as a sperm maturation factor of

study, we found that angiotensin-converting

female. Recently, we star ted new research

enzyme (ACE) releases GPI-anchored proteinsprojects elucidating character and function of(GPI-AP) from the cell membrane and plays a

new he lper T ce ll, T h17 ce ll, to clarif y thecritical role in mammalian fertilization. We alsomechanism of inflammation.

Prof. Gen Kondoh
E-Mail:kondohg@infront.kyoto-u.ac.jp

Assoc. Prof.

Keiji Hirota
E-Mail:hkeiji@infront.kyoto-u.ac.jp

During development of multicellular organisms, genetic stability
is differentially regulated depending on developmental stages,

Assist. Prof.

cellular lineages and physiological conditions etc. We are

Hitomi Watanabe

currently investigating (1) how pluripotent stem cells andE-Mail:(concurrent)

watanabe@infront.kyoto-u.ac.jp

germline cells maintain their genome and epigenome integrity,and (2) how the genome and epigenome stability is coordinatedwith developmental programs of the germline-stem cell cycle.
We also aim to identify genes and pathways with which thegenetic stability of stem cell resources can be improved.

Lab URL

https://www2.infront.kyoto-u.ac.jp/rc01/index-j.htm

Assoc. Prof.

Shinichiro Chuma
E-Mail:schuma@infront.kyoto-u.ac.jp

Lab URL

19

http://an02-kaihen-anim.frontier.kyoto-u.ac.jp/contact.html

20Department of Regeneration Science and Engineering

Prof. Shimon Sakaguchi
E-Mail:shimon@frontier.kyoto-u.ac.jp

Lab. of Experimental
Immunology (Visiting)

Department of Regeneration Science and Engineering

Lab. of Material Biophysics (Visiting)
Individual cellular activities fluctuate, yet are

new fluorescent repor ter (Hes7-Achilles) toconstantly coordinated at the population levelmonitor synchronous HES7 oscillations in the

Our laborator y studies the mechanisms ofcells for clinical application, which is a novelvia cell-cell coupling. A notable example is themouse PSM at single-cell resolution. Wild-typei m mu n o l o g i c a l to l e ra n c e. We d i s c ove re dimmuno-therapy for autoimmune diseases,s o m i te s e g m e nt ati o n c l o c k, i n w h i c h th ecells can rapidly correct phase fluctuations in

Prof. Ryoichiro Kageyama
E-Mail:rkageyam@infront.kyoto-u.ac.jp

naturally occurring regulatory T (Treg) cells as aallergy, infection, organ transplantation and

Program-Specific Assist. Prof.

expression of clock genes, such as Hes7,

HES7 oscillations, whereas absence of the

T-cell subpopulation that is specialized forcancer.

oscillates in synchrony between cells

Notch modulator Lunatic fringe (Lfng) leads to

E-Mail:immun e suppre s s i o n a nd e ng ag e d in th e

We are also studying the pathoge netic

comprising the presomitic mesoderm (PSM).

loss of PSM cell synchrony. Further analyses

Isomura Akihiro

maintenance of immunological self-tolerance

mechanism of rheumatoid arthritis by analyzingThis synchronization depends on the Notch

revealed a delay control mechanism of theaisomura@infront.kyoto-u.ac.jp

and homeostasis. We have been studying the

our newly developed model (SKG mouse). SKG

signaling pathway, and inhibiting this pathway

oscillator y net works involved in somite

mole cular and ce llular basis of Tre g ce lls

mice have a mutation in the ZAP70 gene, which

desynchronizes oscillations, leading to somite

segmentation, indicating that intercellulardevelopment and maintenance, in mice andplays a critical role in T cell recepter signaling.

fusion. However, how Notch signaling regulates

coupling with a proper delay is essential for thehumans by using immunological, genetic andBe cause of this mutaion, SKG mice show

HES7 oscillation synchrony is unknown. Here,synchronized oscillations.

bioinformatics approaches. Since Treg cells arealtered thymic selection and allow a leakage of

we established a live-imaging system using a

involved in various physiological as well as

self-reactive T-cell from the thymus. We are

p a t h o l o g i c a l i m m u n e r e s p o n s e s, we a r e

investigating how such impaired signal

developing various ways to manipulate Treg

transduction causes autoimmune diseases.

Ryoji Kawakami

kawakami.ryoji.4c@kyoto-u.ac.jp

Program-Specific Assist. Prof.
E-Mail:Live imaging of Hes7 oscillations in the mouse presomitic mesoderm (PSM) at the single-cell resolution. Hes7
expressin oscillates synchronously in the wild-type PSM but not in Lfng-KO PSM.

CD25 +CD4 + regulatory T (Treg) cells are produced by the normal thymus as afunctionally mature T-cell subpopulation. T hey spe cif ically express thetranscription factor FoxP3. Reduction of Treg cells or attenuation of theirsuppressive activity may enhance tumor immunity and microbial immunity. In
contrast, incre ase of the number of Tre g ce lls or augme ntation of the ir
suppressive activity can treat autoimmunity and induce transplantation tolerance.

Lab URL

21

http://exp.immunol.ifrec.osaka-u.ac.jp/

22Department of Biosystems Science

Prof. Taiji Adachi
E-Mail:adachi@infront.kyoto-u.ac.jp

Department of Biosystems Science

Lab. of Biomechanics
The Laboratory of Biomechanics aims to clarify

mechanical environment” and “hierarchy ofLab. of Developmental Systems
Organogenesis is a highly dynamic process intissues. Based on our past achievements in 3D

the self-organized regulatory mechanisms of astructure and function” in living systems basedwhich multicellular behaviors are regulated bytissue formations from pluripotent stem cells,

diverse biological phenomena through interdis-

on integrated biomechanics and mechanobiol-

mechanisms in multiple scales from molecules

we have been attempting to extend our limit of

ciplinary approaches encompassing mechan-ogy studies using experiments and mathemati-

and cells to tissues. In vitro generation of func-

understanding for self-organization phenomena

ics, life science, and medical sciences. The

cal modeling and simulation. Our researchtional organ with complex structure is a major

in neural development and advance the culture

major goal of our research is to understandtopics cover developmental processes (cellchallenge of cell biology. Toward this goal, it is atechnology for generation of more complex

how well-organized dynamics emerge fromdifferentiation, morphogenesis, and growth)reasonable strategy to recapitulate the ontoge-tissues from ES/iPS cells in a more robust

complex molecular and cellular interactions inand functional adaptation to the environment by

ny that is the most efficient and robust processmanner. To do that, we mainly focus on follow-

living systems. Specifically, we are focused on

remodeling and regeneration of tissues andfor organogenesis acquired through evolution.

ing points.

highlighting the roles of “adaptation to theorgans.

Our laboratory aims to clarify molecular andcellular mechanisms underlying organogenesis,

Sr. Lect.

Kennedy Omondi Okeyo
E-Mail:Assist. Prof.

Yoshitaka Kameo
E-Mail:kameo@infront.kyoto-u.ac.jp

E-Mail:eiraku@infront.kyoto-u.ac.jp

1) Elucidation of self-organization phenomena

and to develop new technologies for in vitro

in neural development and morphogenesis

recapitulation, that is, three-dimensional func-

2) Development of novel technologies for intional organ generation from stem cells. We

okeyo@infront.kyoto-u.ac.jp

Prof. Mototsugu Eiraku

vitro formation of functional organ

Assoc. Prof.

Masatoshi Ohgushi
E-Mail:mohgushi@infront.kyoto-u.ac.jp

have previously established efficient three-di-3) Molecular analysis of species-specific regu-

mensional cultures for generation of mouse andlation for developmental timing and tissue

Assist. Prof.

human ES/iPS cell-derived brain and retinal

size determination.

E-Mail:ti s su e a s we ll a s oth e r e cto d e r m- d e r i ve d

Yusuke Seto
seto.yusuke.2v@kyoto-u.ac.jp

Assist. Prof.

Koichiro Maki

maki@infront.kyoto-u.ac.jp

Figure 1 Bone can remodel its outer shape and inner structure to adapt to the surrounding
mechanical environment. This study aims to clarify the mechanism of bone functional
adaptation achieved by cooperative cellular activities.

Figure 2 Morphogenesis of biological tissues is regulated by mechanical forces generated
through multicellular interactions. This study aims to clarify the mechanism of tissuemorphogenesis using experiments and simulations.

Lab URL

23

https://www2.infront.kyoto-u.ac.jp/bf05/index-e.html

in vitro formation of optic cup and layered retina from ES cells
a-c, Isolated optic cup structure generated from mouse ES cells. d-f, ES cell-deirved optic
cup differentiate into layered retinal structure. g, Scheme of in vitro optic cup formation in ES
cell culture.
Lab URL

https://www2.infront.kyoto-u.ac.jp/bs01/

24

Department of Biosystems Science

Department of Biosystems Science

Lab. of Growth Regulation System
Our laboratory has been trying to reveal the mechanisms that
regulate proliferation and differentiation of neural stem cells(NSCs) in the brain. Elucidation of this mechanism leads not
only to understanding of brain development and morphogenesis, but also to unravelling of mysteries underlying mammalianbrain evolution. Neurogenesis from NSCs continues in specificregions (such as hippocampus) of the adult brain. If thisprocess can be controlled, it would be possible to apply to theneuroregenerative medicine to countervail the decline in the
cognitive functions caused by aging or neurodegenerative
diseases. Besides, we have also been investigating the mechanism of somitogenesis which leads to the segmented structure

Lab. of Systems Virology
Prof. Yoshio Koyanagi
E-Mail:ykoyanag@infront.kyoto-u.ac.jp

Program-Specific Assoc. Prof.

Yuki Furuse
E-Mail:furuse.yuki.4e@kyoto-u.ac.jp

Virus infects cell and replicates. Viral genome

from the aspects of immunology and virology.

transmits from infected cell to adjacent naive

The main subject of our research is HIV, whichcells. This is a most significant characteristic ofcauses AIDS in human. The mechanism byvirus. The mechanism of this infection event iswhich HIV infection results in AIDS remains

a primary theme of our laboratory. It has been

unclear. We have been investigating how thefound that various cellular factors positively andimmunodeficiency is triggered by HIV infection

negatively associate with viral replication. How-

using in vitro (cell culture system) and in vivo

ever, we do not yet have the answer for how,(animal model) through the application of math-which, and when cellular factors commit viralematics and bioinformatics. We developed areplication. The aim of our laboratory is to learn

humanized mouse system in which the humanthe mechanism how virus replicates in the cells.

immune system is reconstituted.

We address the mechanism of virus replicationof the vertebrate body.

Assoc. Prof.

Toshiyuki Ohtsuka
E-Mail:tohtsuka@infront.kyoto-u.ac.jp
One of the studies aiming to elucidate the process of brain
evolution. Comparison of the size and morphology of thebrains between transgenic (Tg) mice, in which proliferationof neural stem cells are maintained and promoted via
coactivation of Notch- and Shh-signaling, and control (Ctrl)
mice. Upper: bright field images; Lower: GFP fluorescence
images.
Lab URL

https://www2.infront.kyoto-u.ac.jp/Kageyama/

TopicsSr.Lect.Alexis Vandenbon

Lab. of Medical Virology

alexisvdb@infront.kyoto-u.ac.jp

Measles virus is highly contagious and causes immune suppression, leading to high pathoge-

E-Mail:nicity. In spite of its medical importance, the mechanism of infection is not fully understood. As
the virus express two glycoproteins responsible for entry into target cells on its envelope, we
aim to understand the mechanism of the infection by revealing the glycoprotein structures at
the atomic level. Currently, speciﬁc therapeutic agents are not available for measles. Therefore,
the structures can provide the basis for developing antiviral drugs to prevent the infection.
Program-Specific Sr. Lect.

Kazuya Shimura
E-Mail:kshimura@infront.kyoto-u.ac.jp

Assist. Prof.

Tateki Suzuki
Overview of various bioinformatics analyses in our group. (A) A collection of 8,796 humanbulk RNA-seq samples presents a systemic over view of gene expression. (B-C) We
developed a computational method for finding differentially expressed genes in single-cell
RNA-seq data as well as in spatial transcriptomics data. Shown are a single-cell dataset frommouse bone marrow (B), and a spatial transcriptomics dataset from the mouse brain (C).

Lab URL

25

https://www2.infront.kyoto-u.ac.jp/Koyanagi_HP/

A model of measles virus entry
The binding of MV-H to the receptor molecule on an immune cell reduces the distance between the viral envelope
and the cell membrane. The subsequent conformational shift of the MV-H(form I to form II) may allow MV-F toundergo successive structural changes, leading to membrane fusion.

26

Department of Biosystems Science
Assist. Prof.

Department of Biosystems Science

Assist. Prof.

Laboratory of
RNA system

E-Mail:RNAs exist as RNA-Protein complexes, RNPs. We

Makoto Kitabatake
E-Mail:kitabatake@infront.kyoto-u.ac.jp

Ichiro Taniguchi
taniguchi@infront.kyoto-u.ac.jp

study the process by which RNAs are synthesised,processed and matured into RNPs. When RNPs lose
their function due to errors in synthesis or stress in thecell, quality control mechanisms take place to detectand eliminate such molecules. We use biochemistry
to elucidate this mechanism.

Today, the main genetic material is DNA, but themain functional molecules are both proteins andRNAs (RNPs). RNPs go through a cycle from birth
to death, just like the life of a human being. Eachstep in the cycle has an impor tant biologicaltheme.

Lab. of Biological Membrane System

TopicsLab. of Developmental Systems

Lab. of Biomaterials

During the development of animals, complex intercellular inter-

A plasma membrane of a cell and an organelle has remarkably

actions acts as the bases of the formation of well-organizedadvanced functions as nano-devices. Membrane proteins playtissue structures. Those intercellular communications and cellu-

a central role in these nano-devices, however, their hydropho-

lar kinetics evoked by them are highly dynamic, so wee do not

bic nature has often hampered the progression of the mem-yet know the whole picture. To reveal the intercellular interac-brane protein science. In Lab. of Biomaterials, the major focus

tions underlying the tissue formation, I'm attempting to induceof my research is to achieve the development of “membranecell aggregate with tissue-like structures from pluripotent stem

proteins”-based DDS and regenerative therapy through thecells. By identif ying the factors for making well-organized

construction of membrane scaﬀold-biomaterial hybrid com-

structures one by one, I want to reveal the mechanisms under-

posites . To tackle this, I tr y to clarif y their structures andlying the development of animals.

biological functions using a plasma membrane itself and

The research projects carried out in this groupevolutionar y conser ved SecYEG translocon

are concerned with dynamic aspects of cell

associated with auxiliar y factors (such as

sur face proteins in bacteria including

SecDF) and the SecA ATPase motor. We are

Es c h e r i c h i a c o l i a n d V i b r i o a l g i n o l y ti c u s.

inve stigating the str ucture a nd mole cula rSpecif ically, processes of protein folding,

function of these and related cellular factors

protein translocation across and integration intoand re gulation of their expre ssion. (2)the membrane, membrane protein proteolysis

Membrane protein degradation andand extracy toplasmic stress responses are

extracytoplasmic stress response: Membranes t u d i e d by c o m b i n e d m o l e c u l a r g e n e t i c,

proteins play central roles in the functions ofbiochemical biophysical and structural

biological membranes. We are investigating theapproaches. We are mainly focusing on themechanism and physiological roles offollowing two topics. (1) Function and regulation

membrane protein degradation by envelopeof protein translocation machiner y: Protein

proteases. We are also interested in the cellular

ex p o r t a c r o s s t h e b a c te r i a l c y to p l a s m i csystem to sense and cope with abnormality ofmembrane is promoted by cooperation of thecell surface proteins.

Prof. Yoshinori Akiyama
E-Mail:yakiyama @ infront.kyoto-u.ac.jp

Assoc. Prof.

Hiroyuki Mori
E-Mail:hiromori @ infront.kyoto-u.ac.jp
Assist. Prof.

Yohei Hizukuri
E-Mail:yhizukur @ infront.kyoto-u.ac.jp

membrane protein-reconstituted lipid membranes.

Assist. Prof. Yusuke Seto

Assist. Prof. Mitsuru Ando

The research prejects carried out in the laboratory of Biological membrane
system.

Lab URL

27

https://infront-biomembrane.jp28Department of Biosystems Science

Department of Biosystems Science

Lab. of Tissue Homeostasis
Each organ in the adult body responses toand secretory molecules affect the transcrip-

Lab. of Mathematical Biology
The progress of modern biology revealed thatmental verifications. We are promoting multiple

tissue damage or physiological changes of thetional network in the multicellular systems isbiological phenomena are governed by com-projects of collaborations with experimentalb o d y th ro u g h re g u l ati n g th e m u l ti c e l u l l a r

one topic in the projects. These endogenous

plex network systems including many mole-

biologists. One of our recent projects is study-

network by which organ size and functions areti s s u e re m o d e l i n g m e c h a n i s m s wo u l d b ecules, cells or organs. For the aim of under-

ing dynamics of complex network systems indetermined. Our laboratory studies the mecha-

applied for regenerative medicine. We also

standing the functions of complex systems, webiology. We developed some theoretical frame-nisms of tissue remodeling especially focusing

interested in how the maternal tissue remodel-

adopt mathematical and computational meth-works to ex tract the impor tant aspects ofon a regenerating organ from acute and chronic

ing contributes to fetal growth or developmen-

ods. By theoretical approaches we decipher

dynamics from network structure alone, with-damage, as well as maternal remodeling organ

tal origin of health and disease (DOHaD).

huge amounts of experimental information, andout assuming other quantitative details. By

to give integrative understanding for the biologi-

combining our theory with experimental mea-

Riki Ishibashi

cal systems. Our final goal is to open a new

suring and controling, we will determine mech-rishibas@infront.kyoto-u.ac.jp

bioscience which will progress by the repeats

anism of dynamical behaviours and understandof the theoretical predictions and the experi-

the principles for the biological functions.

Prof. Fumiko Toyoshima
E-Mail:ftoyoshi@infront.kyoto-u.ac.jp

Assist. Prof.

Yukako Oda
E-Mail:ykoda@infront.kyoto-u.ac.jp
Assist. Prof.

during pregnancy. How the tissue mechanics

E-Mail:Assist. Prof.

Ryo Ichijyo

Prof.

Atsushi Mochizuki
E-Mail:mochi@infront.kyoto-u.ac.jp

Assoc. Prof.

Masashi Tachikawa
E-Mail:mtach@infront.kyoto-u.ac.jp

E-Mail:richijo@infront.kyoto-u.ac.jp
Program-specific Assist. Prof.

Yoshihiko Kobayashi
E-Mail:kobayashi.yoshihiko.2p@kyoto-u.ac.jp

Research topics in lab. of Mathematical Biology

Lab URL

29

https://www2.infront.kyoto-u.ac.jp/Toyoshima-HP/

Lab URL

http://mathbio.infront.kyoto-u.ac.jp/

30Department of Biosystems Science

Prof. Kosuke Yusa
E-Mail:k.yusa@infront.kyoto-u.ac.jp
Assist. Prof.

Yusuke Tarumoto
E-Mail:ytarumoto@infront.kyoto-u.ac.jp
Assist. Prof.

Gohei Nishibuchi
E-Mail:gnishibuchi@infront.kyoto-u.ac.jp
Assist. Prof.

Kazunari Aoki
E-Mail:aoki.kazunari.4c@kyoto-u.ac.jp
Program-Specific Assist. Prof.

Department of Biosystems Science

Lab. of Stem Cell Genetics
Forward genetic approach can comprehensive-

biology. Our current work focuses on molecularLab. of Cell Fate Dynamics and Therapeutics
My laboratory studies the molecular basis ofoften complex and heterogeneous mixtures ofly reveal genes involved in a phenotype of inter-function studies of genes identified throughcell fate regulation in normal and malignant

neoplastic cells with different self-renewal andest. This approach was frequently applied in

CRISPR-based genetic screening in the follow-

stem cells. We are currently investigating

differentiation capacities. Unlike many differen-

lower model organisms such as yeast, Caenor-

ing two research area: 1. Molecular mecha-

several pathways of hematopoiesis and skeletal

tiated cells within a tumor, some cancer cells

habditis elegans and fruit fly to identify genes

nisms of pluripotency maintenance and differ-muscle systems in mice and human. Stem cells

have the ability to self-renew. These self-re-

involved in fundamental biological processes. In

entiation of human pluripotent stem cells and 2.

have a remarkable ability to propagate them-newing cancer cells, or cancer stem cells, arecontrast, forward genetic approach had been

Genetic vulnerabilities in cancer cells and drug

selves, self-renewal. It allows tissue regenera-

therapy-resistant and can drive tumor relapse

hampered in mammalian cultured cells as there

development. For the latter, we have recently

tion and repair damaged tissue after injury. But

and metastasis following treatment cessation.

was no efficient way to inactivate all copies ofcompleted an analysis of the CRISPR screening

this ability is a double-edged sword; the sameR e c e nt s tu d i e s, i n c l u d i n g o u r ow n wo r k,

every gene. Our research has been focusing on

dataset of > 300 cancer cell lines and identified

mechanism of self-renewal can be a target ofsuggest that the normal and malignant stem

developing novel genetic tools that enable us toa number of promising drug targets. We will

malignant transformation and lead to cancer

cells operate on cell fate regulatory signals that

apply powerful forward genetics in mammalian

conduct detailed molecular studies of thesedevelopment. In the past decades, we haveare common or specific to each population.

cells. We have recently developed a functional

candidates to further narrow down the list tolearned a great deal about the mechanisms of

Our research program seeks to improve ourgenetic screening method using the CRIS-

the most promising drug targets.

cancer-causing transformation, and yet finding

understanding of stem cell and cancer biology,

PR-Cas9 system, which is highly efficient toeffective ways to eradicate cancer cells has

and to apply this knowledge to the develop-

genetically dissect a wide range of mammalianremained an elusive goal in many t ypes ofment of novel and effective approaches to treat

Assist. Prof.

cancers. This is partly because tumors are

human disease and cancer.

E-Mail:Fumihiko Kawamura
E-Mail:kawamura.fumihiko.7x@kyoto-u.ac.jp

Prof.

Takahiro Ito
E-Mail:takahiro.ito@infront.kyoto-u.ac.jp

Assoc. Prof.

Ayuna Hattori
E-Mail:ayu.hattori@infront.kyoto-u.ac.jp

Kenkyo Matsuura
kenkyo.matsuura@infront.kyoto-u.ac.jp

Stem cells in tissues and cancer【Normal tissues】
tissuestem cells【Cancer】
cancerstem cells

Capable of self-renewal
and tumor initiation

Self-renewal anddiﬀerentiaion
in balance

tumor
formation

diﬀerentiation
homeostasis& functional
tissues
Colorectal cancer cell lines showing various cell morphologies. This cancer typecan be classified into a few sub-groups based on gene mutations and gene
expression profiles. Drug targets that show specificity in certain groups are mostvaluable as these targets are associated with biomarkers that can be used forpatient stratification, and prioritised in follow-up analysis and subsequent drug
development process.

regression
and cure}

Tumor regression
due to the lack ofcancer stem cells

Stem cells in tissues and cancer. Stem cells maintain both normal and malignant tissues, and we seek to uncover themolecular basis of cell fate regulation essential for the stem cell functions.

Lab URL

31

Therapies targetingcancer stem cells

https://www.infront.kyoto-u.ac.jp/research/lab41/

32

Department of Biosystems Science

Prof. Itaru Imayoshi(concurrent)
E-Mail:imayoshi.itaru.2n@kyoto-u.ac.jp

Department of Biosystems Science

Lab. of Deconstruction of Stem Cells
The recent discovery of neural stem cells in thedisorder. In our laborator y, by applying theLab. of Regulatory Information (Visiting)
Virus infections, such as influenza A epidemic

proinflammatory cytokines (Figure), which block

adult central nervous system has raised thenovel light-inducible gene expression system,

and Chronic Hepatitis C virus infection are stillviral replication and promote acquired immunity

possibility of repairing the damaged tissue by

we will try developing novel methods to selec-

impor tant diseases and outbreaks of newly

against viruses. Recently we discovered that

recruitment of their latent, endogenous regen-tively and efficiently induce various neural cell

emerging viruses are serious problems for

persistent activation of MDA5 leads toerative potentials. Development of innovative

types from neural stem cells.

modern society. Higher animals, including

lupus-like autoimmune disorder in mice. The

methods that can noninvasively manipulate

More specifically, we will focus on the dynamic

humans, are genetically equipped with mecha-

purpose of our project is to clarify the molecularneural stem cells in the brain has been expect-

expression changes of transcription factors innisms, collectively known as innate immunity, tomechanism underlying the antiviral innate

ed for regenerative medicine of the nervous

neural stem cells and manipulate them by thecounteract viral infections. During the course of

immunity and autoimmunity regulated by RLR,

system. We have recently demonstrated theoptogenetic approach. We will improve thereplication, many viruses generate

and to develop new diagnostic and therapeutic

first success of such an approach in artificial

specificity and efficiency of differentiation of

double-stranded (ds)RNA, which is virtually

tools for the se dise ase s. T his la borator ymanipulation of proliferation and neuronalneural stem cells and direct reprograming

absent in normal cells and likely serves as abelongs to Graduate School of Biostudies.

differentiation of neural stem cells by light. We

processes. We will apply these light-mediated“foreign molecule” in cells. RIG-I, MDA5 andAssociate Professor Okabe studies on regula-a re c u r re ntl y ex te n d i n g th i s re g e n e r ati ve

control methods to neural stem cells in the

LGP2, collectively termed as RIG-I-Like Recep-

tion of tissue-resident macrophage specializa-

approach to various types of neural disease

brain and iPS cells-derived brain organoids, as

tors (RLRs) function as sensor for viral dsRNAtion and tissue homeostasis.

models in mice and primates, such as traumat-

well as to cultured neural stem cells.

to initiate production of interferon (IFN) andProf. Takashi Fujita
E-Mail:tfujita@infront.kyoto-u.ac.jp

ic injur y, neurodegeneration or psychiatricDevelopment of novel optical methods to regulate differentiation of neural stem cells in neuralstem cell cultures, brain organoids, and living brains.

Lab URL

33

Sensing viral dsRNA and activation of RLRWhen cells were infected with virus (1), viral dsRNA is sensed by RIG-I or MDA5 (2). CARD of
RIG-I and MDA5 interacts with another CARD-containing protein expressed on mitochondria,
termed Interferon Promoter Stimulator-1 (IPS-1) (3). AS a result of these molecular interactions,transcription factors, IRF-3, IRF-7 and NF-κB are activated (4). These transcription factors
cooperatively activate several antiviral genes, including those of type I and type III interferon
are activated (5).

https://brainnetworks.jimdofree.com/

34

Research Center for Infectious Diseases

Research Center for Infectious Diseases

Lab. of Primate Model
Since the nonhuman primate is the closest experimental animal
to human, it is expected to establish the most useful model forhuman infectious disease in many aspects. Some pathogenic
viruses (HIV-1, for example) can only infect primates. We have alarge scale facility for infection experiments using nonhumanprimates at P3 level. We establish infection and disease development models using macaque monkeys, and carry out thebasic research for clarifying the in vivo pathogenesis and developing prevention and cure of infectious diseases.

Assoc. Prof.

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Tomoyuki Miura 

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E-Mail:tmiura@infront.kyoto-u.ac.jp��������������
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We will elucidate the pathogenicity and develop
preventive and therapeutic methods of infectious
diseases by comprehensive analysis at the levelof m o l e c u l e s, c u l t u re d c e l l s, a n d i n fe c te dindividuals.

Lab URL

 !

          The virus mutated in monkeys acquired neutralization
resistance by structural shield of the target site.

Lab. of Infectious Disease Model
Our laboratory is focusing on intractable viruses

infection. In this point of view, we have chal-such as human immunodeficiency virus, hepati-

lenged these issues and established novel

tis C virus and human T-cell leukemia virus.

non-human primate models for the intractable

T hese viruses share common similarities;

viruses. With the use of the model animals, we

disease development after long-term persistent

would like to unravel the molecular and immu-

infection, presence of unique mechanism forn o l o g i c a l m e c h a n i s m s by w h i c h th e v i r a l

the immune evasion, and narrow and selective

persistency and disease onset are induced,

host range. Especially, the last one leads us toand further challenge applied research regard-

be incapable of employing small laborator y

ing the development of vaccines and new

animals as immunocompetent models for viraltherapeutics.

Prof. Hirofumi Akari(concurrent)
E-Mail:akari.hirofumi.5z@kyoto-u.ac.jp

https://www2.infront.kyoto-u.ac.jp/primatemodelHP/

TopicsLab. of Cell Fate Dynamics and Therapeutics
Since my arrival in Oct 2020, I have been working on how RNA binding proteins regulate cancer
progression both in vitro and in vivo using cancer mouse models and human patient samples. Our
goal is to understand how our cells respond to intrinsic and extrinsic factors such as genetic
Figure 1 Interaction between HIV-1 Nef N-terminus
and mu-1 subunit of adaptor protein-1

mutations, stresses, nutrients in homeostasis, aging and diseases.

Assoc. Prof.

Ayuna Hattori

Figure 2 histopathological analysis ofliver fibrosis in a tamarin
persistently infected with
G BV- B ( M a s s o n's tr i c h ro m estaining)

Lab URL

35

https://akari-labo.jimdo.com/

36Research Center for Infectious Diseases

Research Center for Infectious Disease

Lab. of Virus-HostCoevolution

Endogenous retroviruses (ERVs) occupy about 10% of mammalian genomes. New exogenous retroviruses arise from ERVs
by recombination and induce diseases in the new hosts. On
the other hand, cetrain ERVs are known to be involved inplacental morphogenesis and reprogramming of somatic cells.
In this laboratory, we aim to reveal the mechanisms of theemergence of new viral diseases and the process of coevolution between mammals and viruses.

Assoc. Prof.

Animal Experiment Facility for Viral Infection

E-Mail:We have a laboratory facility for investigation of biological reactions with virus infection. The purpose of this facility is to analyze

Takayuki Miyazawa
takavet@infront.kyoto-u.ac.jp

pathogenicity of human pathogenic viruses and develop useful vaccines. BSL2 and BSL3 rooms are in operation at each pathogen
level. Small animals such as mice and medium animals such as monkeys can be used as experimental animals. After permission fromthe committee of faculty and technical staff (veterinarians), viral infection experiments are conducted under strict control.

Fematrin-1, a bovine endogenous retrovirus K1-derived protein, is involved in the formation of trinucleate
cells (TNC) appreared in bovine placenta. BNC: binucleate cells; MTC: mononucleate trophoblast cells.

TopicsLab URL

https://paleovirology.jimdo.com/

Lab. of Immunology
Cell type-specific gene expression is driven through the interplay between lineage-spciefic transcription factors (TFs) and thechromatin architecture, including enhancer-promoter inteactions. To elucidate the molecular mechanisms of the cell type-specific funcsitons, it is important to understand thisinterplay. Current technical innovations enable us to clarify the large scale topological regulation of chromatin architecutre and the underlying mchanisms of how lineage-specific TFs orchestrates the traget geneloci. We take advantage of these techniques to address these fundamental questions regarding gene regulation during adaptive
lymphocyte development and activation, and will aim at unraveling the pathologies of human disease in the future.

Reproductive
Engineering Team
Reproductive engineering team is a support unit for generatingtransgenic mouse (Tg) and knockout mouse (KO). We also
perform cryopreservation of mouse-fertilized eggs.

Program-Specific Researcher

Kazuko Miyazaki

Hi-C contact maps. Red dots indicate the chromatin interactions. Wild-type cells show the elaborating
chromatin interactions in this genomic region (red triangle), which indicate the chromatin looping structure (upper panel). Enhancer deletion cells lose this chromatin structure, suggesting the importance ofenhancer activity in orchestration of genome confromations.

37

40

38

Center for Animal Experiments

Infection Experiment Facility for SARS-CoV-2
We perform research activity of SARS-CoV-2 that has spreadover the world. Experiments are conducted in BSL3 facility witha special ventilation system. Thus, the infection experiments ofthis virus can be carried out safely.

Prof. Gen Kondoh(concurrent)
E-Mail:kondohg@infront.kyoto-u.ac.jp
Assoc. Prof.

Keiji Hirota

Experimental animals, such as mouse, rat andm o re ra p i d l y a n d c o nve n i e ntl y. R e c e ntl y,others, are housed in our Center under strictg e n o m e e n g i n e e r i n g m eth o d s h ave b e e nregulation of animal experimental committee

established using TALEN or CRISPR-Cas9

and institutional guidelines for animal welfare.

systems. We have searched for many methods

Moreover, we have been considered for longand finally developed our own protocol making

time: how to make gene-manipulated micesuch mice more easily and reproducibly.

(concurrent)
E-Mail:hkeiji@infront.kyoto-u.ac.jp
Assist. Prof.

Hitomi Watanabe
E-Mail:watanabe@infront.kyoto-u.ac.jp

TopicsLab. of Deconstruction of Stem Cells
Tens to hundreds of billions of neurons form a huge neural network in our brain. These neurons perform complex information processing to achieve functions such as cognition, learning, and memory. To elucidate the circuit mechanisms of thesecomplex higher brain functions, we are developing optical technologies to visualize and manipulate neuronal activities andthe dynamics of their downstream signaling molecules with high spatiotemporal resolution. We hope our new optical technology will enable us to capture new biological phenomena beyond the framework of conventional physiology and lead tothe elucidation of the pathogenesis of mental and neurological disorders and drug discovery.

Our strategy for developing genome-edited mice using CRISPR-Cas9 system.

Lab URL

https://www2.infront.kyoto-u.ac.jp/an/newpage1.html

Multi-color calcium imaging

Program-Specific Assoc. Prof.

Masayuki Sakamoto(Graduate School of
Biostudies, Kyoto University)

39

40

Center for Human ES Cell ResearchCenter for human ES cell research
Division of Clinical Basis for ES Cell ResearchLab. of Embryonic
Stem Cell Research

Associate professor Suemori

・Establishment and distribution of hES cell lines
・Library construction of hES cell lines

Lab. of Embryonic
Stem Cell Application

Associate professor Chuma

・Quality control of hES cell lines
・Comparative analyses of genome/epigenome regualion(full time)(concurrent)

Division of Basic Technology Development for ES Cell ResearchLab. of
Organoids Technology

About us

Our mission

Professor Eiraku(Center Director/concurrent)

Associate professor Ohgushi(concurrent)

Lab of regenerative
immune cell therapy

Professor Kawamoto

Lab of ES celldifferentiation

Professor Yusa

(concurrent)(concurrent)

・Generation of organoids from hES cells
・Regenerative medicine & drug discovery by organoids
・Regeneration of T cells from hES cells
・Cancer immunotherapy by regenerative T cells
・Analyses of hES cell differentiation and lineage commitment
・Development of hES cell differentiation methods

Organization overview

The center for human ES cell research comprises two divisions, namely, the division of clinical basis for ES cell research and the division of

The center for human ES cell research was newly established in April 2020, with a mission of promoting the establishment and distribution ofbasic technology development for ES cell research. The division of clinical basis for ES cell research consists of two groups: the laboratory ofhuman ES (ES) cell lines and the advancement of applied studies on them. ES cells are a type of pluripotent stem cells, known as widely asembryonic stem cell research (associate professor Suemori), which is responsible for the establishment and distribution of human ES cell

induced-pluripotent stem (iPS) cells, and human ES cells precede human iPS cells in the history of their first establishment. There are twolines, and the laboratory of embryonic stem cell application (associate professor Chuma), which takes charge of quality control of and compar-

institutes in Japan approved for the generation of hES cell lines, one being the national center for child health and development and the otherative genome/epigenome analyses of human ES cell lines. The division of basic technology development for ES cell research consists of three

being our institute, and currently we are manufacturing human ES cell lines for clinical use in our MHLW approved cell processing facility.

groups, which aim for research and development, with mid-to long-term vision, intended for clinical applications of human ES cells: the labora-

Regarding the importance of human ES cells in academic researches and their potential benefits in clinical applications, a robust and stabletory of organoids technology (professor Eiraku, Center Director and associate professor Ohgushi), the laboratory of regenerative immune cell

supply of high-quality human ES cells is essential for the advancement of regenerative medicine as well as basic researches. To realize thetherapy (professor Kawamoto) and the laboratory of ES cell differentiation (professor Yusa). Together, our center works toward the establish-

clinical application of human ES cells, we strengthen our current facility of ES cell production and, through further cooperation with otherment of an international and stable research facility for human ES cell distribution, as well as the development of basic technologies required

research organizations and hospitals inside and outside of Japan, we accelerate the progression of stem cell research and regenerative medi-

for its clinical application, such as quality control and cell/organoid culturing methods.

cine.

History

Institute for frontier life and medical sciences first succeeded in establishing human ES cell lines in 2002, and since 2017, directed by the theformer laboratory of embryonic stem cell research, has been serving as the supplier of clinical-grade human ES cell lines in Japan (as of April,2020). In order to enhance our capability and performance in research and development of human ES cells for clinical use, the facility underwent a reorganization in 2020, and the center for human ES cell research was newly founded.

Division of Clinical Basisfor ES Cell ResearchLab. of Embryonic Stem Cell Research

Human ES cell lines are considered to have great potential in
medical research and application such as cell transplantation
therapy and drug discovery. We established human ES celllines at a high efficiency and analyzed their characters in detail.
We derived 5 ES cell lines, named KhES-1, KhES-2, KhES-3,
KhES-4 and KhES-5, and distributed to over 50 research
projects in Japan. We are also per forming researches onmolecular mechanisms of self-renewal and differentiation ofhuma n ES c e lls, a nd deve loping te chniqu e s for g e netic
manipulation of hES cells.
We have constructed a Cell Processing Facility (CPF) to develop
core technologies to produce and supply clinical grade humanembryonic stem (hES) cell lines.

Assoc. Prof.

Hirofumi Suemori
E-Mail:hsuemori @ infront.kyoto-u.ac.jp

Program-Specific Sr.Lect.

Eihachiro Kawase
E-Mail:Human Embryonic Stem Cell

Clinical-grade hESC Processing Facility
Lab URL

41

kawase8 @ infront.kyoto-u.ac.jp

https://www2.infront.kyoto-u.ac.jp/es01/top.htm
42

to Kyoto International
Conference Center

Map & Access

Access to inFront

❸

❷
❺
❶

University Hospital

Kawabata St.

❼❹

ImadegawaKarasuma St.

Keihan Jingu-Marutamachi Sta.
Exit 5

Kawaramachi St.

It takes about 80 minutes andcosts 3,370 yen.

Kawabata St.

❸

Karasuma Oike
Kawaramachi

Shijo St.
HankyuKyoto Line

Shijo/Karasuma

・Lab. of Tumor Viruses
・Lab. of Molecular and Cellular Biology
・Lab. of Biomaterials
・Lab. of Immunology
・Lab. of Tissue Regeneration
・Lab. of Developmental Epigenome
・Lab. of Integrative Biological Science・Lab. of Experimental Immunology
・Lab. of Biomechanics
・Lab. of Developmental Systems
・Lab. of Stem Cell Genetics
・Administration Oﬃce❷

Institute for Frontier Life andMedical Sciences Bldg. No.2

・Lab. of Medical Virology
・Lab. of Tumor Viruses
・Lab. of Cell Regulation
・Lab. of Immune Regulation
・Lab. of Material Biophysics
・Lab. of Systems Virology
・Lab. of Growth Regulation System
・Lab. of RNA System
・Lab. of Biological Membrane System
・Lab. of Tissue Homeostasis
・Lab. of Deconstruction of Stem Cells
・Lab. of Regulatory Information
・Lab. of Primate Model・Lab. of Virus-Host Coevolution
・Department of Cell Biology

43

Sanjō Keihan
Gion-Shijo
JR Line

JR Kyoto Sta.

❺

Keihan Line
SubwayKarasuma Line

South Research Bldg. No.1
Institute for Frontier Life andMedical Sciences Bldg. No.1

Jingu-Marutamachi

Oike St.

Kasugakami St.

Ta k e J R K a n s a i - A i r p o r t
Express “HARUKA” to Kyoto
Station.

Marutamachi St.

SubwayTozai Line❶

Kyoto University

Marutamachi❻● Fro m K a n s a i I nte r n ati o n a l
Airport (KIX) by Train

Demachiyanagi

Imadegawa St.

●From Kyoto Station by Taxi
It takes 20 minutes and costs
2,000 yen, approximately.

●From Kyoto Station by City Bus
Take a No. 206 bus bound for“Higashiyama St. and Kitaoji
Bus Terminal”, and get off at“Kumano Jinja-mae”. Walk two
blocks to the west. It takes 5
minutes.
●From Kyoto Station by Subway
Take Subway Karasuma Lineand get off at “Marutamachi”.
Walk east for about 20 minutes.

TofukujiInstitute for Frontier Life andMedical Sciences Bldg. No.3

・Lab. of RNA Viruses
・Lab. of Ultrastructural Virology
・Lab. of Systems Virology
・Lab. of Mathematical Biology
・Lab. of Cell Fate Dynamics and Therapeutics
・Reproductive Engineering Team・Center for Animal Experiments❹

Institute for Frontier Life andMedical Sciences Bldg. No.4

・Lab. of Immune Regulation
・Center for Animal Experiments

Frontier Life and Medical Sciences Research Fund
Institute for Frontier Life andMedical Sciences Bldg. No.5

・Lab. of Integrative Biological Science・Lab. of Developmental Systems
・Division of clinical basis for ES cell research

Uncovering the secrets of vital activity to shape the future of medical sciences
The Institute for Frontier Life and Medical Sciences, Kyoto University is known for its brilliant researchﬁndings in medical sciences, including the discovery of the human leukemia virus and regulatory T cells.
Your contribution will help us move forward.

◆Please visit the Frontier Life and Medical Sciences Research Fund website.
http:// www.kikin.kyoto-u.ac.jp/en/contribution/infront/❻

Molecular Biology Research Bldg.

・Animal Experiment Facility for Viral Infection❼ Institute for Frontier Life andMedical Sciences
North Research Bldg.

・Lab. of Deconstruction of Stem Cells
・Lab. of Infectious Disease Model

SARS-CoV-2（EM was taken by Prof. Noda's group）.

Human ES cell line was produced for clinical purposes at
the ﬁrst time in Japan (KthES11). It has been already
distributed to various research institutions.

44