Jpn. J. Infect. Dis., 53, 2000

Laboratory and Epidemiology Commumications
Outbreak of Salmonella Enteritidis Caused by ContaminatedBuns Peddled by a Producer Using Traveling Cars in Hyogo

and Neighboring Prefectures in 1 999: an EpidemiologlCal●Study Using Pulsed‑Field Gel Electrophoresis
Hidetaka Tsuji, Kunio Shimada, Kokichi Hamada* and Hiroshi Nakajimal
Division of Microbiology, Hyogo Prefectural Institute of Public Health,Arata‑cho 2‑I‑29, Hyogo‑ku, Kobe 652‑0032 andJDepartment of Microbiology, Okqyama Prefectural lnstitute for Environm ental
Science and Public Health,
Uchio 739‑1, Okqyama 701‑0298, Japan
Communicated by Shunsuke lmai

(Accepted March 24, 2000)

Salmonella serovarEnteritidis has become the most preva‑

lentamong the Salmonella serotypesinJapanSince 1989 (I).

TowninOkayama Prefecture. Thirteen Salmonella Enteritidis
isolates were obtained from fecal specimens of 206 patients

In September 1999, an outbreak of the serovar occurred inwith diarrhea, abdominal palnS, fever, and in some cases,

Okayama Prefecture and its boundary areas extending toHyogoand Tottori Prefectures (Fig. 1). The outbreak was duenausea. Two isolates were obtained丘omthe food.

All 15 isolates showed a phagetype ofRDNC (Reactionto contaminated creambuns peddled by a producer using eightnoes 斑ot Conform) (2). Almost allthe isolates were sensitive

traveling cars. They were produced by a manufacturerinSeto

to CP, ABPC, CPFX, FOM, GM, ST,and TMP; one isolate
showedintermediate resistance to CP,three isolates to ABPC,and one strainto CPFX. They wereintemediately resistant*Corresponding author: Fax: +8 1 178‑53 1 ‑708023Jpn. J. Infect. Dis., 53, 2000

Fig･ 2･ PFGE pattcm showing BlnI‑digested chromosomaI DNA ofSalmonella Enteritidis isolates.

Lane I; A DNA ladder: lanes 2‑12; isolates &om patients: lane 13;

an isolate from a creambun.

food which is commoninJapan. This epidemic showed theinherent risk of this method in spreading food poISOnlng

Fig. 1. Location of outbreak.

throughout a large geographical area.

The authors are grateful to Drs･ Hidemasa Izumlya andHaruo Watanabe, National Institute of Infectious Diseases,
Tokyo, for the phagetyping of our isolates and for a criticalto SM, TC, KM, and NA, except fわr three isolates whichwere sensitive. They were also intemediately resistant toCTX, except f♭r fわur isolates which were sensitive. None ofthe isolates werefully resistant to the 12 antibiotics tested.
Antibiotic resistance was assayedinthis study by uslng Sensi
Disc (BD Biosciences, Sparks, Md, USA).
Fourteen isolates were examined by pulsed‑field gel electro‑

reading of the manuscript.

REFERENCES
1. National Institute of Health and Infectious Diseases

phoresis (PFGE) by using a Gene PathTyping System (Nippon
Bio‑Rad, Tokyo). The PFGE pattem ofBlnI‑digested chromo‑

ControI Division, Ministry ofHealthand Welfare ( 1 995):

Somal DNAs was identical f♭r all the isolates (Fig. 2). TheSalmonella, Japan, 1 992‑ 1 994. Infect. Agents SuⅣeillance

PFGE pattem ofXbaI digests demonstrated essentially theRep. 16, 1'‑2'.

2. Ward, L R., de Sa, J. D. H.andRowe, B. (1987):Aphage‑same results (data not shown).

The cream buns were made of butter and chicken eggs.

typing scheme for Salmonella enteritidis･ Epidemiol･The producer used cars to peddle them, a method of selling

Infect., 99, 291‑294.

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