The heliOS network
Network of autonomous heliX® modules, simply plug-and-play.
Reliable and robust by built-in system redundancy for uninterrupted operation.

Combine as many heliX® modules as you require
to scale-up throughput to your needs.

ObserverOperator

Highly-automated THROUGHPUT
Ease-of-Use

4 signals, real-time
4 single-photon counters for highest
fluorescence sensitivity.
Data collection at 10 ms to resolve
even the fastest kinetics in real-time.

High PERFORMANCE
sensingswitchSENSE® static and dynamic
measurement modes for the analysis of binding kinetics and molecularconformations.

Robust microfluidics

heliX®
single unit

Simplistic single-channel design,
made from durable glass, withstanding highest flow rates and corrosive
chemicals.
Disposable, maintenance-free.

Automatic
chip loader

Powerful
software5 chips, automatically exchangeable and NFC-tagged for seamless traceability.

For efficient planning and analysisof binding and conformation experiments.

Autosampler
384 and 96 well plates.
Sample temperature 10 – 70°C,
sample compartment 4 – 40°C.

Intuitive for the novice and configurable for the expert analysis of
big data.
Control and monitor heliX® modules from anywhere.

DISCOVER
MOLECULAR
INTERACTIONS
Kinetics – Affinity – Avidity
Conformational Changes
Thermodynamics

The heliOS network
Network of autonomous heliX® modules, simply plug-and-play.
Reliable and robust by built-in system redundancy for uninterrupted operation.

Combine as many heliX® modules as you require
to scale-up throughput to your needs.

ObserverOperator

Highly-automated THROUGHPUT
Ease-of-Use

4 signals, real-time
4 single-photon counters for highest
fluorescence sensitivity.
Data collection at 10 ms to resolve
even the fastest kinetics in real-time.

High PERFORMANCE
sensingswitchSENSE® static and dynamic
measurement modes for the analysis of binding kinetics and molecularconformations.

Robust microfluidics

heliX®
single unit

Simplistic single-channel design,
made from durable glass, withstanding highest flow rates and corrosive
chemicals.
Disposable, maintenance-free.

Automatic
chip loader

Powerful
software5 chips, automatically exchangeable and NFC-tagged for seamless traceability.

For efficient planning and analysisof binding and conformation experiments.

Autosampler
384 and 96 well plates.
Sample temperature 10 – 70°C,
sample compartment 4 – 40°C.

Intuitive for the novice and configurable for the expert analysis of
big data.
Control and monitor heliX® modules from anywhere.

DISCOVER
MOLECULAR
INTERACTIONS
Kinetics – Affinity – Avidity
Conformational Changes
Thermodynamics

heliX® system comparison

switchSENSE® – Comprehensive biophysical information,in one measurement

association and dissociation rate constants

protein conformation

DNA / RNA

melting point

sample integrityTwo-color fluorescencesensing & molecular
dynamics measurement

Kinetics – Affinity – Avidity

High sensitivity (fmol/l)
Low sample consumption

Conformations
switchSENSE®

One chip for
multiple applications

heliX

heliX+

Double-heliX

4-heliX Bundle

No. of chips,
auto-exchangeable

1

5

10

20

multispecific interactions (antibodies)

No. of real-time signals

2

4

8

16

Proteins hydrodynamic protein diameter

Real-time referencing[1] same channel[1] same channel[1] same channel[2] different channels

cooperativity

No. of well-plates / wells

1 / 96

1 / 384

2 / 768

Fluorescence channels

One color

Two Colors

Sampling rate

1 datapoint/s

100 datapoints/s

Temperature[1] Tconst.= 25°C or 37°C

[1] any constant temperature from 10° to 70°C
[2] variable temperature, ramp speed up to 10°C/min, for Tm measurement

Measurement modes[1] Molecular Dynamics[2] Fluor. Proximity Sens.

[1] Molecular Dynamics (molecular friction)[2] Fluorescence Proximity Sensing (FPS)[3] Fluorescence resonance energy transfer (FRET)

Kinetics

ka = 103 … >107 M-1s-1
kd = 10-6 … 0.2 s-1
KD = 0.1 pM – 1 mM

ka = 103 … >108 M-1s-1
kd = 10-6 … 20 s-1
KD = 50 fM – 1 mM

interlinking

Thermodynamics

affinity vs. avidity

concentration measurements

screening

agglomeration

folding / unfolding

activity of nucleic acid enzymes

small molecule induced conformational changes

Small
Molecules
sequence specificity

MEASUREMENTS APPLICATIONS

inhibitor dose response
free energy, enthalpy, entropy
monomer-multimer transitions

4 / 1536

heliX® system comparison

switchSENSE® – Comprehensive biophysical information,in one measurement

association and dissociation rate constants

protein conformation

DNA / RNA

melting point

sample integrityTwo-color fluorescencesensing & molecular
dynamics measurement

Kinetics – Affinity – Avidity

High sensitivity (fmol/l)
Low sample consumption

Conformations
switchSENSE®

One chip for
multiple applications

heliX

heliX+

Double-heliX

4-heliX Bundle

No. of chips,
auto-exchangeable

1

5

10

20

multispecific interactions (antibodies)

No. of real-time signals

2

4

8

16

Proteins hydrodynamic protein diameter

Real-time referencing[1] same channel[1] same channel[1] same channel[2] different channels

cooperativity

No. of well-plates / wells

1 / 96

1 / 384

2 / 768

Fluorescence channels

One color

Two Colors

Sampling rate

1 datapoint/s

100 datapoints/s

Temperature[1] Tconst.= 25°C or 37°C

[1] any constant temperature from 10° to 70°C
[2] variable temperature, ramp speed up to 10°C/min, for Tm measurement

Measurement modes[1] Molecular Dynamics[2] Fluor. Proximity Sens.

[1] Molecular Dynamics (molecular friction)[2] Fluorescence Proximity Sensing (FPS)[3] Fluorescence resonance energy transfer (FRET)

Kinetics

ka = 103 … >107 M-1s-1
kd = 10-6 … 0.2 s-1
KD = 0.1 pM – 1 mM

ka = 103 … >108 M-1s-1
kd = 10-6 … 20 s-1
KD = 50 fM – 1 mM

interlinking

Thermodynamics

affinity vs. avidity

concentration measurements

screening

agglomeration

folding / unfolding

activity of nucleic acid enzymes

small molecule induced conformational changes

Small
Molecules
sequence specificity

MEASUREMENTS APPLICATIONS

inhibitor dose response
free energy, enthalpy, entropy
monomer-multimer transitions

4 / 1536