δ H

δ C

δ N

15

δ 18O and δ 34S(mean value in ‰ vs. VSMOW,± 1σ) (range)(# of measurements)(mean value in ‰ vs. VPDB, ± 1σ)(range)(# of measurements)(mean value in ‰ vs. AIR,± 1σ) (range)(# of measurements)(mean values in ‰ vs.
VSMOW or VCDT,± 1σ) (range)(# of measurements)

Acetanilide #1, C8H9NO,
CAS # 103-84-4, in glass vial,
5 g US $250, 2 g US $150

not determined(contains exchangeable hydrogen)-29.53 ± 0.01 ‰from -29.51 to -29.54 ‰n=6+1.18 ± 0.02 ‰from +1.16 to +1.21 ‰n=4

not determined

Acetanilide #2, C8H9NO,
CAS # 103-84-4, in glass vial,
2 g US $250

not determined(contains exchangeable hydrogen)-29.50 ± 0.02 ‰from -29.48 to -29.53 ‰n=4+19.56 ± 0.03 ‰from +19.53 to +19.60 ‰n=7

not determined

Acetanilide #3, C8H9NO,
CAS # 103-84-4, in glass vial,
2 g US $250

not determined(contains exchangeable hydrogen)-29.50 ± 0.02 ‰from -29.49 to -29.52 ‰n=4+40.57 ± 0.06 ‰from +40.52 to +40.66 ‰n=6

not determined

L-Alanine, C3H7NO2, CAS # 56-41-7,
produced by SI Science in Japan, 100
mg in crimp-sealed glass vial, US $250

not determined(contains exchangeable hydrogen)-17.93 ± 0.02 ‰from -17.90 to -17.96 ‰n=5+43.25 ± 0.07 ‰from +43.16 to +43.34 ‰n=4

not determined

Benzoic acid #A, C7H6CO2,
CAS # 65-85-0; inquire about availability

not determined(contains exchangeable hydrogen)-28.81 ‰Coplen et al., 2006
https://doi.org/10.1021/ac052027c

not applicable+23.14 ± 0.19 ‰Brand et al., 2009http://dx.doi.org/10.1002/r
cm.3958

not determined(contains exchangeable hydrogen)-28.85 ‰Coplen et al., 2006
https://doi.org/10.1021/ac052027c

not applicable+71.28 ± 0.36 ‰Brand et al., 2009http://dx.doi.org/10.1002/r
cm.3958+96.9 ± 0.9 ‰n = 53-35.05 ± 0.04 ‰n = 114-2.87 ± 0.04 ‰n = 93

2

Version 3 September 2024

Materials for EA-IRMS
formula, CAS #, purity, amount, type of
packaging, price in US $Structure

Benzoic acid #B, C7H6CO2,
enriched in

18

O, CAS # 65-85-0; inquire
about availability

Caffeine #1, USGS61, C8H10N4O2,
CAS # 58-08-2, ≥99 %, anhydrous, 500
mg in glass vial, US $275

(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analche
392)
392)
m.5b04392)-156.1 ± 2.1 ‰n = 64

Caffeine #2, USGS62, C8H10N4O2, CAS# 58-08-2, ≥99 %, anhydrous, 500 mg inglass vial, US $275

+174.5 ± 0.9 ‰n = 55

special procedures need to
be followed when using thisreference material for H, O,
and S isotope ratios. See:
https://doi.org/10.1021/acs.jaf
c.0c02610

Corn starch, (CH2O)n, ≥99.5 %, CAS #9005-25-8, 1 g in glass vial,
US $150.

-1.17 ± 0.04 ‰n = 103(+20.1 ± 6.3 ‰ for nonexchangeable H when
following USGS procedure)n = 12(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)(-43.7 ± 7.8 ‰ for nonexchangeable H when
following USGS procedure)n = 12(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)

not determined(contains exchangeable hydrogen)components of oil may havesolidified at low storage
temperature; gently warm
sealed ampoule to liquefy
and homogenize oil prior to
opening

Coumarin, C9H6O2, ≥99.5 %, CAS # 9164-5, 100 mg in crimp-sealed glass vial,
US $250

Eicosanoic acid methyl ester (C20:0)#Y, methyl eicosanoate #Y, C21H42O2,≥99 %, CAS # 1120-28-1, at least 50
mg in sealed glass vial, US $250

+20.17 ± 0.06 ‰n = 96+37.83 ± 0.06 ‰n = 99(Anal. Chem. , 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analche
392)
392)
m.5b04392)

special procedures need to
be followed when using thisCollagen powder from porcine origin, reference material for H, O,
and S isotope ratios. See:
USGS89, 0.5 g in glass vial, US $275
https://doi.org/10.1021/acs.jaf
c.0c02610

Corn oil from USA, USGS87,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)-14.79 ± 0.04 ‰n = 105(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analche
392)
392)
m.5b04392)

Caffeine #3, USGS63, C8H10N4O2, CAS# 58-08-2, ≥99 %, anhydrous, 500 mg inglass vial, US $275

Collagen powder from wild-caught
marine fish, USGS88, 0.5 g in glass
vial, US $275

13-168.1 ± 2.7 ‰n = 34(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)+82.3 ± 1.2 ‰+80.9 to +83.7 ‰n=4

CH3(CH2)18COOCH3+3.7 ± 0.8 ‰from +2.4 to +4.1 ‰
n=4

from

-16.06 ± 0.07 ‰n = 54+14.96 ± 0.14 ‰n = 50not determined

not determined

not determined(+15.91 ± 0.44 ‰+17.10 ± 0.44 ‰when following USGS
pre-drying procedure)n = 18 n = 12

(J. Agricult. Food Chem ., 2020, 68 ,(J. Agricult. Food Chem ., 2020, 68 , 10852;
10852;
https://doi.org/10.1021/acs.jafc.0c02610) https://doi.org/10.1021/acs.jafc.0c0(https://doi.org/10.1021/acs.jafc
2610).0c02610)-18.13 ± 0.11 ‰n = 64+6.25 ± 0.12 ‰n = 48(+8.37 ± 0.40 ‰+3.86 ± 0.56 ‰when following USGS
pre-drying procedure)n = 20 n = 12

(J. Agricult. Food Chem ., 2020, 68 ,(J. Agricult. Food Chem ., 2020, 68 , 10852;
10852;
https://doi.org/10.1021/acs.jafc.0c02610) https://doi.org/10.1021/acs.jafc.0c0(https://doi.org/10.1021/acs.jafc
2610).0c02610)-11.01 ± 0.02 ‰from -10.99 to -11.03 ‰n=4-15.51 ± 0.09 ‰n = 35(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)

not applicable

not determined

not determined+20.11 ± 0.85 ‰n = 12(https://doi.org/10.1021/acs.jafc.0c02610)-35.60 ± 0.01 ‰from -35.59 to -35.61 ‰n=3

not applicable

not determined-0.73 ± 0.02 ‰from -0.70 to -0.75 ‰n=4

not applicable

not determined

δ H

δ C

δ N

15

δ 18O and δ 34S(mean value in ‰ vs. VSMOW,± 1σ) (range)(# of measurements)(mean value in ‰ vs. VPDB, ± 1σ)(range)(# of measurements)(mean value in ‰ vs. AIR,± 1σ) (range)(# of measurements)(mean values in ‰ vs.
VSMOW or VCDT,± 1σ) (range)(# of measurements)-183.9 ± 1.4 ‰n = 116-30.53 ± 0.04 ‰n = 77

not applicable

not determined

not applicable

not determined

not applicable

not determined

2

Version 3 September 2024

Materials for EA-IRMS
formula, CAS #, purity, amount, type of
packaging, price in US $Structure

Eicosanoic acid methyl ester (C20:0)#Z1, methyl eicosanoate #Z1,
USGS70, C21H42O2, ≥99.5 %, CAS #1120-28-1, 100 mg in glass vial, US$275

CH3(CH2)18COOCH3

Eicosanoic acid methyl ester (C20:0)#Z2, methyl icosanoate #Z2, USGS71,
C21H42O2, monoatomic 2H and 13C
spikes in methyl group, ≥99.5 %, CAS #1120-28-1, 100 mg in glass vial, US$275

CH3(CH2)18COOCH3

Eicosanoic acid methyl ester (C20:0)#Z3, methyl icosanoate #Z3, USGS72,
C21H42O2, monoatomic 2H and 13C
spikes in methyl group, ≥99.5 %, CAS #1120-28-1, 100 mg in glass vial, US$275

CH3(CH2)18COOCH3

13(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
http://dx.doi.org/10.1021/acs.analchem.5b http://dx.doi.org/10.1021/acs.analchem.5b0
04392)
4392)-4.9 ± 1.0 ‰n = 118-10.50 ± 0.03 ‰n = 65(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04
392)
392)

+348.3 ± 1.5 ‰n = 130-1.54 ± 0.03 ‰n = 62(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04
392)
392)

EDTA #2, ethylene diamine
tetraacetic acid, C10H16N2O8, CAS # 6000-4, 99 %, 2 g in glass vial, US $250

not determined(contains exchangeable hydrogen)-40.38 ± 0.01 ‰from -40.37 to -40.38 ‰n=4-0.83 ± 0.04 ‰from -0.78 to -0.88 ‰n=6

not determined

9-Ethylcarbazole, C14H13N, ≥99.5%,CAS # 86-28-2, ≥200 mg in crimpsealed glass vial, US $250

-102.0 ± 1.1 ‰from -100.6 to -103.6 ‰
n=7-25.36 ± 0.02 ‰from -25.35 to -25.39 ‰n=5+3.93 ± 0.06 ‰from +3.87 to +4.00 ‰n=5

not applicable(-13.9 ± 2.4 ‰ for nonexchangeable H when
following USGS procedure)n = 12-13.75 ± 0.06 ‰n = 51

Flour from Italian millet, USGS90,
0.5 g in glass vial, US $275

special procedures need to
be followed when using thisreference material for H, O,
and S isotope ratios. See:
https://doi.org/10.1021/acs.jaf
c.0c02610

special procedures need to
be followed when using thisFlour from Vietnamese rice, USGS91, reference material for H, O,
and S isotope ratios. See:
0.5 g in glass vial, US $275
https://doi.org/10.1021/acs.jaf
c.0c02610(J. Agricult. Food Chem. , 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)(-45.7 ± 7.4 ‰ for nonexchangeable H when
following USGS procedure)n = 12(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)+8.84 ± 0.17 ‰n = 42(+35.90 ± 0.29 ‰-15.14 ± 0.67 ‰when following USGS
pre-drying procedure)n = 14 n = 12

(J. Agricult. Food Chem ., 2020, 68 ,(J. Agricult. Food Chem ., 2020, 68 , 10852;
10852;
https://doi.org/10.1021/acs.jafc.0c02610) https://doi.org/10.1021/acs.jafc.0c0(https://doi.org/10.1021/acs.jafc
2610).0c02610)-28.28 ± 0.08 ‰n = 63+1.78 ± 0.12 ‰n = 70(+21.13 ± 0.44 ‰-20.85 ± 0.72 ‰when following USGS
pre-drying procedure)n = 14 n = 12

(J. Agricult. Food Chem ., 2020, 68 ,(J. Agricult. Food Chem ., 2020, 68 , 10852;
10852;
https://doi.org/10.1021/acs.jafc.0c02610) https://doi.org/10.1021/acs.jafc.0c0(https://doi.org/10.1021/acs.jafc
2610).0c02610)

D-glucose, C6H12O6, ≥99 %,CAS # 5099-7, produced by SI Science in Japan,
100 mg in crimp-sealed glass vial,
US $250

not determined(contains exchangeable hydrogen)-133.06 ± 0.1 ‰from -132.96 to -133.16 ‰n=5

not applicable

not determined

L-Glutamic acid, ≥99.5 %,CAS # 56-86-0, 2 g in glass vial,
US $250

not determined(contains exchangeable hydrogen)-28.60 ± 0.01 ‰from -28.58 to -28.61 ‰n=5-2.38 ± 0.04 ‰from -2.32 to -2.42 ‰n=4

not determined

Glyceryl tripalmitate, C51H98O5, ≥99.0%, CAS # 555-44-2, at least 5 mg in
crimp-sealed glass vial, US $250

-215.1 ± 0.9 ‰from -214.1 to -216.1 ‰
n=4-30.12 ± 0.01 ‰from -30.10 to -30.12 ‰n=3

not applicable

not determined

Glycine #1, USGS64, C2H5NO2, ≥99.5%, CAS # 56-40-6, 500 mg in glass vial,
US $275

not determined(contains exchangeable hydrogen)-40.81 ± 0.04 ‰n = 89+1.76 ± 0.06 ‰n = 98Glycine #2, USGS65, C2H5NO2, ≥99.5%, CAS # 56-40-6, 500 mg in glass vial,
US $275

not determined(contains exchangeable hydrogen)

Glycine #3, USGS66, C2H5NO2, ≥99.5%, CAS # 56-40-6, 500 mg in glass vial,
US $275

not determined(contains exchangeable hydrogen)

Glycine #4, C2H5NO2, ≥99.5 %, CAS #56-40-6, produced by SI Science inJapan, ≥99.9 % by 1H NMR, 100 mg in
crimp-sealed glass vial, US $250

not determined(contains exchangeable hydrogen)(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analche392)
m.5b04392)-20.29 ± 0.04 ‰n = 86+20.68 ± 0.06 ‰n = 92(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analche392)
m.5b04392)-0.67 ± 0.04 ‰n = 96+40.83 ± 0.06 ‰n = 92(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analche392)
m.5b04392)-60.02 ± 0.02 ‰,from -60.00‰ to -60.06‰;
n=5-26.63 ± 0.02 ‰,from -26.61‰ to -26.65‰;
n=3

not determined

not determined

not determined

not determined

δ H

δ C

δ N

15

δ 18O and δ 34S(mean value in ‰ vs. VSMOW,± 1σ) (range)(# of measurements)(mean value in ‰ vs. VPDB, ± 1σ)(range)(# of measurements)(mean value in ‰ vs. AIR,± 1σ) (range)(# of measurements)(mean values in ‰ vs.
VSMOW or VCDT,± 1σ) (range)(# of measurements)-259.2 ± 1.3 ‰from -257.5 to -261.0 ‰n=7-29.95 ± 0.02 ‰from -29.92 to -29.97 ‰n=8

not applicable

not applicable2

Version 3 September 2024

Materials for EA-IRMS
formula, CAS #, purity, amount, type of
packaging, price in US $Structure

13

Hexatriacontane #2, C36 n -alkane #2,
C36H74, CAS # 630-06-8, 100 mg in
crimp-sealed glass vial, US $250

CH3(CH2)34CH3

Honey from Vietnam, USGS82,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)honey crystallized at low
storage temperature; gently
warm sealed ampoule toliquefy and homogenize
honey prior to opening(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)

Honey from Canada, USGS83,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)honey crystallized at low
storage temperature; gently
warm sealed ampoule toliquefy and homogenize
honey prior to opening(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)+3.7 ± 0.8 ‰from +2.4 to +4.1 ‰n=4-0.72 ± 0.02 ‰from -0.70 to -0.74 ‰n=3-183.9 ± 1.4 ‰n = 116-30.53 ± 0.04 ‰n = 77

Icosanoic acid methyl ester (C20:0)#Y, methyl icosanoate #Y, C21H42O2,
2

H and 13C spikes in fatty acid: 1,1-(2H2),

CH3(CH2)18COOCH3

1-(13C), ≥99 %, CAS # 1120-28-1, 50
mg in sealed glass vial, US $250

-43.1 ± 3.7 ‰n = 20-110.5 ± 3.5 ‰n = 19-24.31 ± 0.08 ‰n = 44(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)-26.20 ± 0.08 ‰n = 44

Icosanoic acid methyl ester (C20:0)#Z1, methyl icosanoate #Z1, USGS70,
C21H42O2, ≥99.5 %, CAS # 1120-28-1,
100 mg in glass vial, US $275

CH3(CH2)18COOCH3

Icosanoic acid methyl ester (C20:0)#Z2, methyl icosanoate #Z2, USGS71,
C21H42O2, monoatomic 2H and 13C
spikes in methyl group, ≥99.5 %, CAS #1120-28-1, 100 mg in glass vial, US$275

CH3(CH2)18COOCH3(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04
392)
392)

Icosanoic acid methyl ester (C20:0)#Z3, methyl icosanoate #Z3, USGS72,
C21H42O2, monoatomic 2H and 13C
spikes in methyl group, ≥99.5 %, CAS #1120-28-1, 100 mg in glass vial, US$275

CH3(CH2)18COOCH3(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04
392)
392)

Olive oil from Italy, Sicily, USGS84,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)components of oil may havesolidified at low storage
temperature; gently warm
sealed ampoule to liquefy
and homogenize oil prior to
opening(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)

Olive oil from Peru, USGS85,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)components of oil may havesolidified at low storage
temperature; gently warm
sealed ampoule to liquefy
and homogenize oil prior to
opening(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)

Peanut oil from Vietnam, USGS86,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)components of oil may havesolidified at low storage
temperature; gently warm
sealed ampoule to liquefy
and homogenize oil prior to
opening

Polyethylene powder, USGS77, lowdensity, 1000 μm, CAS # 9002-88-4, 1 g
in glass vial, US $275

(CH2CH2)nPolyethylene line NDF-PE77 (extruded
from powder USGS77; isotopicallyindistinguishable from powder), lowdensity, CAS # 9002-88-4, inquire about
availability or contact Tamim Darwish(ndf-enquiries@ansto.gov.au)(CH2CH2)nL-Phenylalanine, C9H11NO2, ≥99.5 %,CAS # 63-91-2, produced by SI Sciencein Japan, 100 mg in crimp-sealed glass
vial, US $250

(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04
392)
392)

-4.9 ± 1.0 ‰n = 118+348.3 ± 1.5 ‰n = 130-140.4 ± 3.1 ‰n = 34-158.6 ± 2.7 ‰n = 34-207.4 ± 4.5 ‰n = 34-10.50 ± 0.03 ‰n = 65-1.54 ± 0.03 ‰n = 62-28.80 ± 0.09 ‰n = 35(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)-29.74 ± 0.08 ‰n = 36(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)-30.63 ± 0.09 ‰n = 36(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)-75.9 ± 0.6 ‰n = 199-30.71 ± 0.04 ‰n = 81(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04
392)
392)

indistinguishable from USGS77(see above)

indistinguishable from USGS77(see above)(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
http://dx.doi.org/10.1021/acs.analchem.5b http://dx.doi.org/10.1021/acs.analchem.5b0
04392)
4392)

not determined

not determined+19.44 ± 0.36 ‰n = 17(https://doi.org/10.1021/acs.jafc.0c02610)+18.20 ± 0.25 ‰n = 15(https://doi.org/10.1021/acs.jafc.0c02610)

not applicable

not determined

not applicable

not determined

not applicable

not determined

not applicable

not determined

not determined

not determined

not determined+26.36 ± 0.50 ‰n = 23(https://doi.org/10.1021/acs.jafc.0c02610)+22.00 ± 0.60 ‰n = 17(https://doi.org/10.1021/acs.jafc.0c02610)+18.76 ± 1.03 ‰n = 19(https://doi.org/10.1021/acs.jafc.0c02610)

not applicable

not applicable

not applicable

not applicable

not determined(contains exchangeable hydrogen)-11.20 ± 0.02 ‰from -11.19 to -11.23 ‰n=6+1.70 ± 0.06 ‰from +1.64 to +1.77 ‰n=5

not determined-81.9 ± 1.2 ‰from -81.8 to -83.0 ‰n=4-29.98 ± 0.01 ‰from -29.96 to -29.99 ‰n=3

not applicable

not determined

Phthalic acid #2, C8H6O4, CAS # 88-993, δ 2H measured in Na-phthalate to
exclude carboxyl hydrogen. δ 13C
measured in free acid. 3 g in glass vial,
US $250

δ H

δ C

δ N

15

δ 18O and δ 34S(mean value in ‰ vs. VSMOW,± 1σ) (range)(# of measurements)(mean value in ‰ vs. VPDB, ± 1σ)(range)(# of measurements)(mean value in ‰ vs. AIR,± 1σ) (range)(# of measurements)(mean values in ‰ vs.
VSMOW or VCDT,± 1σ) (range)(# of measurements)

Phytol, C20H40O, ≥97 %, CAS # 754149-3, 0.5 mL sealed under argon inglass ampoule, US $250

-102.2 ± 2.5 ‰from -98.9 to -105.8 ‰n =5-32.17 ± 0.01 ‰from -32.17 to -32.18 ‰n=5

not applicable

not determined

L-Proline, C5H9NO2, ≥99.5 %, CAS #147-85-3, 100 mg in crimp-sealed glass
vial, US $250

not determined(contains exchangeable hydrogen)-12.47 ± 0.01 ‰from -12.45 to -12.49 ‰n=5-7.84 ± 0.04 ‰from -7.77 to -7.88 ‰n=5

not determined

Starch from corn, (CH2O)n, ≥99.5 %,CAS # 9005-25-8, 1 g in glass vial,
US $150.

not determined(contains exchangeable hydrogen)-11.01 ± 0.02 ‰from -10.99 to -11.03 ‰n=4

not applicable

not determined

Urea #1, CH4N2O, ≥99.5 %, CAS # 5713-6, 2 g in glass vial, US $250

not determined(contains exchangeable hydrogen)-34.13 ± 0.03 ‰from -34.17 to -34.09 ‰n=6+0.26 ± 0.03 ‰from +0.20 to +0.28 ‰n=7

not determined

Urea #2a, CH4N2O, ≥99.5 %, CAS # 5713-6, 2 g in glass vial, US $250

not determined(contains exchangeable hydrogen)-9.14 ± 0.02 ‰from -9.11 to -9.17 ‰n = 10+20.73 ± 0.04 ‰from +20.67 to +20.78 ‰n=9

not determined

Urea #3a, CH4N2O, ≥99.5 %, CAS # 5713-6, 2 g in glass vial, US $250

not determined(contains exchangeable hydrogen)+5.89 ± 0.03 ‰from +5.85 to +5.93 ‰n=5+42.05 ± 0.03 ‰from +42.02 to +42.10 ‰n=5

not determined-75.9 ± 0.6 ‰n = 199-30.71 ± 0.04 ‰n = 81

not applicable

not applicable

not applicable

not applicable2

Version 3 September 2024

Materials for EA-IRMS
formula, CAS #, purity, amount, type of
packaging, price in US $USGS77, polyethylene powder, lowdensity, 1000 μm, CAS # 9002-88-4, 1 g
in glass vial, US $275

Structure(CH2CH2)n13(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04
392)
392)

2
hydrocarbon oil mixture,+397.0 ± 2.2 ‰-29.72 ± 0.04 ‰USGS78, vacuum pump oil #2, Hvapor pressure @ 25 °C
n = 200n = 80(Anal.
spiked with perdeuterated n 0.000133 Pa, viscosity 65 cSt(Anal. Chem ., 2016, 88 , 4294.
Chem ., 2016, 88 , 4294.
2
tetracosane (99.1 atom % H), 1 mL in @ 40 °C, specific gravity 0.78 https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04
g/cm3
sealed glass ampoule, US $275
392)
392)

-43.1 ± 3.7 ‰n = 20

USGS82, honey from Vietnam,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)honey crystallized at low
storage temperature; gently
warm sealed ampoule toliquefy and homogenize
honey prior to opening(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)

USGS83, honey from Canada,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)honey crystallized at low
storage temperature; gently
warm sealed ampoule toliquefy and homogenize
honey prior to opening(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)

USGS84, olive oil from Sicily, Italy,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)components of oil may havesolidified at low storage
temperature; gently warm
sealed ampoule to liquefy
and homogenize oil prior to
opening(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)

USGS85, olive oil from Peru,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)components of oil may havesolidified at low storage
temperature; gently warm
sealed ampoule to liquefy
and homogenize oil prior to
opening(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)

USGS86, peanut oil from Vietnam,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)components of oil may havesolidified at low storage
temperature; gently warm
sealed ampoule to liquefy
and homogenize oil prior to
opening(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)

USGS87, corn oil from USA,
1 mL sealed under argon in glass
ampoule, US $275 (also available from
USGS in crimp-sealed silver tubing)components of oil may havesolidified at low storage
temperature; gently warm
sealed ampoule to liquefy
and homogenize oil prior to
opening(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)

USGS88, marine collagen powder
from wild-caught fish, 0.5 g in glass
vial, US $275

special procedures need to
be followed when using thisreference material for H, O,
and S isotope ratios. See:
https://doi.org/10.1021/acs.jaf
c.0c02610-110.5 ± 3.5 ‰n = 19-140.4 ± 3.1 ‰n = 34-158.6 ± 2.7 ‰n = 34-207.4 ± 4.5 ‰n = 34-168.1 ± 2.7 ‰n = 34(+20.1 ± 6.3 ‰ for nonexchangeable H when
following USGS procedure)n = 12(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)-24.31 ± 0.08 ‰n = 44(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)-26.20 ± 0.08 ‰n = 44(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)-28.80 ± 0.09 ‰n = 35(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)-29.74 ± 0.08 ‰n = 36(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)-30.63 ± 0.09 ‰n = 36(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)-15.51 ± 0.09 ‰n = 35(J. Agricult. Food Chem ., 2020, 68 , 10852;
https://doi.org/10.1021/acs.jafc.0c02610)-16.06 ± 0.07 ‰n = 54not determined

not determined

not determined

not determined

not determined

not determined+14.96 ± 0.14 ‰n = 50+19.44 ± 0.36 ‰n = 17(https://dx.doi.org/10.1021/acs.j
afc.0c02610)+18.20 ± 0.25 ‰n = 15(https://dx.doi.org/10.1021/acs.j
afc.0c02610)+26.36 ± 0.50 ‰n = 23(https://dx.doi.org/10.1021/acs.j
afc.0c02610)+22.00 ± 0.60 ‰n = 17(https://dx.doi.org/10.1021/acs.j
afc.0c02610)+18.76 ± 1.03 ‰n = 19(https://dx.doi.org/10.1021/acs.j
afc.0c02610)+20.11 ± 0.85 ‰n = 12(https://dx.doi.org/10.1021/acs.j
afc.0c02610)(+15.91 ± 0.44 ‰+17.10 ± 0.44 ‰when following USGS
pre-drying procedure)n = 18 n = 12

(J. Agricult. Food Chem ., 2020, 68 ,(J. Agricult. Food Chem ., 2020, 68 , 10852;
10852;
https://doi.org/10.1021/acs.jafc.0c02610) https://doi.org/10.1021/acs.jafc.0c0(https://dx.doi.org/10.1021/acs.j
2610)
afc.0c02610)

δ H

δ C

δ N

15

δ 18O and δ 34S(mean value in ‰ vs. VSMOW,± 1σ) (range)(# of measurements)(mean value in ‰ vs. VPDB, ± 1σ)(range)(# of measurements)(mean value in ‰ vs. AIR,± 1σ) (range)(# of measurements)(mean values in ‰ vs.
VSMOW or VCDT,± 1σ) (range)(# of measurements)(-43.7 ± 7.8 ‰ for nonexchangeable H when
following USGS procedure)n = 12-18.13 ± 0.11 ‰n = 64

2

Version 3 September 2024

Materials for EA-IRMS
formula, CAS #, purity, amount, type of
packaging, price in US $Structure

USGS89, porcine collagen powder,
0.5 g in glass vial, US $275

special procedures need to
be followed when using thisreference material for H, O,
and S isotope ratios. See:
https://doi.org/10.1021/acs.jaf
c.0c02610

USGS90, millet flour from Italy,
0.5 g in glass vial, US $275

special procedures need to
be followed when using thisreference material for H, O,
and S isotope ratios. See:
https://doi.org/10.1021/acs.jaf
c.0c02610

USGS91, rice flour from Vietnam,
0.5 g in glass vial, US $275

special procedures need to
be followed when using thisreference material for H, O,
and S isotope ratios. See:
https://doi.org/10.1021/acs.jaf
c.0c02610

hydrocarbon mixture, vapor

Vacuum pump oil #1, NBS 22a, 1 mL pressure @ 25 °C 0.000133
Pa, viscosity 65 cSt @ 40 °C,
in sealed in glass amoule, US $275
specific gravity 0.78 g/cm3(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)(-13.9 ± 2.4 ‰ for nonexchangeable H when
following USGS procedure)n = 12(J. Agricult. Food Chem. , 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)(-45.7 ± 7.4 ‰ for nonexchangeable H when
following USGS procedure)n = 12(J. Agricult. Food Chem ., 2020, 68 ,10852;
https://doi.org/10.1021/acs.jafc.0c02610)-120.4 ± 1.0 ‰n = 20313+6.25 ± 0.12 ‰n = 48(J. Agricult. Food Chem ., 2020, 68 ,(J. Agricult. Food Chem ., 2020, 68 , 10852;
10852;
https://doi.org/10.1021/acs.jafc.0c02610) https://doi.org/10.1021/acs.jafc.0c0(https://dx.doi.org/10.1021/acs.j
2610)
afc.0c02610)+8.84 ± 0.17 ‰n = 42-13.75 ± 0.06 ‰n = 51# 516-06-3, 99 %, 500 mg in glass vial,
US $275(+35.90 ± 0.29 ‰-15.14 ± 0.67 ‰when following USGS
pre-drying procedure)n = 14 n = 12

(J. Agricult. Food Chem ., 2020, 68 ,(J. Agricult. Food Chem ., 2020, 68 , 10852;
10852;
https://doi.org/10.1021/acs.jafc.0c02610) https://doi.org/10.1021/acs.jafc.0c0(https://dx.doi.org/10.1021/acs.j
2610)
afc.0c02610)+1.78 ± 0.12 ‰n = 70-28.28 ± 0.08 ‰n = 63(+21.13 ± 0.44 ‰-20.85 ± 0.72 ‰when following USGS
pre-drying procedure)n = 14 n = 12

(J. Agricult. Food Chem ., 2020, 68 ,(J. Agricult. Food Chem ., 2020, 68 , 10852;
10852;
https://doi.org/10.1021/acs.jafc.0c02610) https://doi.org/10.1021/acs.jafc.0c0(https://dx.doi.org/10.1021/acs.j
2610)
afc.0c02610)-29.72 ± 0.04 ‰n = 103(Anal.
(Anal. Chem ., 2016, 88 , 4294.
Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04
392)
392)

+397.0 ± 2.2 ‰-29.72 ± 0.04 ‰Vacuum pump oil #2, USGS78, 2Hhydrocarbon mixture, vaporn = 200n = 80spiked with perdeuterated n pressure @ 25 °C 0.000133(Anal.
Chem
.,
2016,
88
,
4294.
(Anal.
Chem ., 2016, 88 , 4294.
2
tetracosane (99.1 atom % H), 1 mL in Pa, viscosity 65 cSt @ 40 °C, https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analchem.5b04
specific gravity 0.78 g/cm3
sealed in glass amoule, US $275
392)
392)
L-Valine #1, USGS73, C5H11NO2, CAS(+8.37 ± 0.40 ‰+3.86 ± 0.56 ‰when following USGS
pre-drying procedure)n = 20 n = 12-24.03 ± 0.04 ‰n = 130
not determined
Chem ., 2016, 88 , 4294.
(contains exchangeable hydrogen)(Anal.

not applicable

not applicable

not applicable

not applicable-5.21 ± 0.05 ‰n = 91(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analche392)
m.5b04392)-9.30 ± 0.04 ‰L-Valine #2, USGS74, C5H11NO2, CAS# 516-06-3, 99 %, 100 mg in glass vial,
freeze-dried, US $275

n = 94not determined
Chem ., 2016, 88 , 4294.
(contains exchangeable hydrogen)

L-Valine #3, USGS75, C5H11NO2, CAS# 516-06-3, 99 %, 100 mg in glass vial,
freeze-dried, US $275

not determined(contains exchangeable hydrogen)(Anal.

+30.19 ± 0.07 ‰n = 68(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analche392)
m.5b04392)+0.49 ± 0.07 ‰n = 23+61.53 ± 0.14 ‰n = 29(Anal. Chem ., 2016, 88 , 4294.
(Anal. Chem ., 2016, 88 , 4294.
https://doi.org/10.1021/acs.analchem.5b04 https://doi.org/10.1021/acs.analche392)
m.5b04392)

not determined

not determined

not determined