Cells and Cell Culture®

Enhanced Superovulation Reagent for mouse
NEW! Also available in convenient freeze-dried format forGreat for IVF.

Use CARD HyperOva

refrigerated storage!!
to obtain more ovulated oocytes.
Live IVF pups from A SINGLE C57BL/6J female
following superovulation with CARD HyperOva®

Product Outline
CARD HyperOva® (Japanese Patent No. 5927588) is an ultra-superovulation reagent that induces ovulation at higher eﬃciency
when compared with the conventional superovulation induction method (PMSG-hCG method). following the conventional
method, we were only able to obtain approximately 20 ova per mouse (C57BL/6J) on average; using CARD HyperOva®,
however, we can obtain as many as 3-4 times more ova.

EﬀectsⅠ. Reduction in Experimental Animal Use (Contribution to 3Rs )Using CARD HyperOva® enables us to reduce the number of female mice used for ovum collection by 1/3-1/4.

Ⅱ. Optimization of Genetically Modiﬁed mouse Creation and Preservation
More ova can be obtained from less female mice using CARD HyperOva® than following the conventional method, which
will facilitate in vitro fertilization and embryo transfer.
Ⅲ. Application to Valuable Genetically modiﬁed Mice
Using CARD HyperOva® on your valuable genetically modiﬁed mice enables you to obtain a lot of ova from small numberof females, thus enabling the large-scale preparation of embryos.
No.

Strains

1

C57BL/6J

2

BALB/c ByJ

3

C3H/HeJ4

DBA/2J

5FVB/NJ

6

CD1

Number of
inseminatedoocytes

Average number ofovulated oocytes/
femaleNo. of fertilized
eggs

Fertilization rate(%)

101,072

27.7 ± 5.4

107.2 ± 22.7

267

10903

29.3 ± 8.9963

96.4 ± 3.2

90.3 ± 14.5

213

73.4 ± 11.3

MethodNumber ofoocyte donors

eCG

10eCG

10eCG

10Product
Product
ProducteCG

Product
eCG

Product
eCG

Product

10101010101010277

293

286

520

225688

168

256

202

337

28.6 ± 8.4

52.0 ± 18.1
22.5 ± 7.8

68.8 ± 13.6
16.8 ± 3.3

25.6 ± 5.9
20.2 ± 8.0

33.7 ± 9.9

680

251

446

194

592

159

239
146

269

89.8 ± 3.7

76.4 ± 9.0

87.8 ± 22.6

85.8 ± 15.5
86.2 ± 11.0
86.0 ± 4.5

94.6 ± 6.7

93.3 ± 6.6

72.3 ± 16.0

79.8 ± 19.6

7.5 IU of CARD HyperOva® or PMSG was injected to four weeks old female mice, then 7.5 IU of hCG was administered 48 hours later. Thereafter all ova
obtained 17 hours after the hCG injection were collected.
● Thefertilization rate is deﬁned as the number of 2-cell embryos divided by the total number of ova (mean±SD).
● Data cited from a paper published by Prof. Naoki Nakagata and Toru Takeo (Center for Animal Resources and development, Kumamoto University).
●Cells and Cell Culture

Transferringof embryos

Birthof pupsIn vitro fertilization®

ResearchOvum collection♂

♀
Preincubation

Cryopreservationof spermatozoa

Thawingof spermatozoa

Preservation

Superovulation Procedure:
1. Inject 0.1-0.2 mL CARD HyperOva i.p. into a 26-30 days old female mouse (birthdate = 0). Injections are usually
performed during the light cycle, between 17:00 and 18:00.
2. At 48 hours after CARD HyperOva(not included).

recipients are injected i.p. with 7.5 IU human chorionic gonadotropin (hCG)

References:
1. Takeo T., Nakagata N. 2015. Superovulation using the combined
administration of inhibin antiserum and equine chorionicgonadotropin increases the number of ovulated oocytes in C57BL/6
female mice.
10(5): e0128330.
doi:10.1371/journal.pone.0128330
2. Takeo T., Nakagata N. 2016. Immunotherapy using inhibin antiserum
enhanced the eﬃcacy of equine chorionic gonadotropin onsuperovulation in major inbred and outbred mice strains.
doi:10.1016/j.theriogenology.2016.04.076
Description
CARD HyperOva®

Cat. No.

Quantity

Storage

KYD-010-EX

1 mL

-20℃

KYD-010-EX-X5

5x1 mL-20℃

Cat. No.

Quantity

Storage

KYD-015-FD-EX

1 x 2 mL

4℃

KYD-015-FD-2-X5

5 x2 mL

4℃

Description
CARD HyperOva® FD

Distributed Worldwide by:

10177

2792 Loker Ave W, Suite 101
Carlsbad, CA 92010
TEL & FAX: 760-431-4600
email : info@cosmobiousa.com
web : www.cosmobiousa.com
For research use only. Not for diagnostic use.

www.cosmobio.com