Products for
IVF
andAssisted Reproduction
Related Procedures
available from Cosmo Bio USA

KYUDO: Complete 2023 product catalog of the most widely utilized andtrusted reagents and accessories for RODENT sperm cryopreservation andassociated IVF procedures. Developed in conjunction with the Center for
Animal Reproduction at Kumamoto University.
Cosmo Bio brand reagents and solutions for RODENT IVF-related procedures. First quality reagents packaged in volumes that may better fit yourworkflow and budget.

Cosmo Bio brand reagents and solutions for IVF-related procedures PIG
IVF-related procedures.

4BioDx: “4MID“ line of ELISA Kits measures proAKAP4, an emerging
demonstrated reliable marker of functional sperm quality and male fertility. Kits for 10 species including agricultural livestock.

Cosmo Bio knows that consistent success in reproductive engineering depends on a myriad of highspec, quality-controlled reagents as well as their proper handling and storage at each step along
the supply chain. All of us at Cosmo Bio take great pride in the quality of our products and greatcare to insure that the materials you receive are always properly cared for.

Cosmo Bio USA, Carlsbad, CA 92010+1 760-431-4600

www.cosmobiousa.com

info@cosmobiousa.com

KYUDO“best reagents” for “best research”

IVF

-19６℃

Cryopreservation

Embryo Transfer

Cutting-edge technology brought to you
mouse
Cold Transport Kit♂

Mouse Sperm
Cryoprotectant
Agent

mouse

♀

CARD HyperOva F.D.
CARD HyperOva

P7

P3
P2

4℃

Mouse Sperm-196℃

Cold storage

Cryopreservation

Preincubation Medium

Superovulation
1M DMSO

P2

Sperm preincubation

DAP213

CARD MEDIUM
ｍHTF

P4
-196℃

P2・P4

1M DMSO

Cryopreservation

In vitro fertilization

0.25M

DAP213

Sucrose

P4

P4
KSOM

-196℃

Cryopreservation

Embryo culture
0.25M

SucroseP4

P４
Embryo transfer

P7
Birth of pups

P1

Reagents for Reproductive Technology of Mouse
CARD FERTIUP Mouse Sperm Cryoprotectant AgentCARD FERTIUP Mouse Sperm Cryoprotectant Agent mitigates freezing damage
during sperm cryopreservation and improves in vitro fertilization rates using
frozen-thawed sperm in numerous mouse strains, including the C57BL/6 strain.
This agent also facilitates a massive reduction in costs associated with theupkeep of strains in comparison to the cost of live animal maintenance.(①)＊Reference(p９)：No.1

CARD FERTIUP Mouse Sperm Preincubation Medium
CARD FERTIUP Mouse Sperm Preincubation Medium promotes
capacitation of mouse sperm and improves in vitro fertilization
rates using fresh, frozen-thawed and cold stored mouse sperm.(②)＊Reference(p９)：No.2＊Patent(JP:4683408）

CARD MEDIUM Mouse Fertilization Medium
CARD MEDIUM High-Performance Mouse IVF Medium brings about amassive improvement in the in vitro fertilization rates of numerous mousestrains, including the C57LB/6 and 129 strains. In fact, using CARD
MEDIUM alongside CARD FERTIUP Mouse Sperm Preincubation Medium
can yield in vitro fertilization rates of over 80% for frozen-thawed
C57BL/6 mouse sperm.(③)＊Reference(p９)：No.3＊Patent（JP:5439677|US:9,994,868 B2）
Fertilization rate of frozen-thawed C57BL/6 sperm

obtain fertilization rates of over 80%

using frozen-thawed C57BL/6 sperm!

Fertilization rate（％）

With CARD MEDIUM, we can even

89.2% （①）（②）（③）

100
８０
６０
４０
２０

17.9%

０
R18S3+HTF

CARD FERTIUP+CARD MEDIUM

P2

CARD HyperOva Superovulation Reagent for Mouse
CARD HyperOva Mouse Superovulation Reagent enables us toobtain a much larger number of oocytes from females of numerous
mouse strains than via conventional superovulation methods.
CARD HyperOva may bring about a threefold or even fourfold
reduction in the required number of donor mice. This contributes
to a reduction in the number of animals per experiment, thereby
upholding the 3 Rs Principle(④)＊Reference(p９)：No.4・No.5＊Patent（JP:5927588｜US:10,500,275,B2｜EU:3260535）

CARD HyperOvaF.D. Superovulation Reagent for Mouse
CARD HyperOva F.D. retains the high performance of
CARD HyperOva while also oﬀering signiﬁcantly improved
storage stability through freeze drying. After preparing thereagent, cryopreserve it at a temperature at -20ºC or
below, and then take as much of the reagent as you need,whenever you need it.(⑤)

Using CARD HyperOva, we can obtain three to four times more

oocytes than conventional superovulation methods in C57BL/6 mice!

Number ofovulated oocytes

120

４０

０

PMSG+hCG■

CARD HyperOve+hCG

68

52

８０
６０

■

90

100

27

24

29

28

7

２０

P3

（④）
（⑤）

107

140

C57BL6/J

A/J

BALB/cByJ

C3H/HeJ22

DBA/2J

1625

FVB/NJ

25

33

CD1

CARD mHTF medium
CARD mHTF Medium is an in vitro fertilization
medium. This medium enables superior in vitro
fertilization rates and a high yield of 2-cellembryos.

CARD KSOMCARD KSOM is a culture medium
for use on embryos obtained via

in vitro fertilization. CARD KSOM
contributes to superior development
rates from 2-cell embryos to blastocysts.

CARD １M DMSOCARD 1M DMSO is cryopreservation
reagent utilized in simple vitriﬁcation.
It is used in the cryopreservation ofembryos and oocytes.

CARD DAP213

CARD DAP213

CARD DAP213 is cryopreservation
reagent utilized in simple vitriﬁcation.
It is used in the cryopreservation ofembryos and oocytes.

CARD 0.25M Sucrose
CARD 0.25M Sucrose is used to warm
vitriﬁed embryos and oocytes via simple
vitriﬁcation.

P4

Breaking new boundaries - Our new technology
Rats

♂

Rats

♀

CARD FERTIUP Rat Sperm
Cryopreservation Agent

P6
-196℃

Cryopreservation
Rat Sperm Freezing Kit

P7

Rat IVF Medium

Superovulation
P6

In vitro fertilization

Embryo culture

Embryo transfer
P7
Birth of pups

P5

Reagents for Reproductive Technology of Rats
CARD FERTIUP Rat Sperm Cryoprotectant AgentCARD FERTIUP Rat Sperm Cryopreservation Agent is the worldʼs ﬁrst practicalcryopreservation agent for use with rat sperm. Previously rat sperm cryopreservation
was an extremely diﬃcult task, but CARD FERTIUP Rat Cryopreservation Agent can
now make it possible to cryopreserve rat sperm in an eﬃcient manner. Whatʼs more,
once thawed the rat sperm maintain a high motility and can be used in in vitro
fertilization.

◆Store at -20℃ or below.
◆For laboratory use only. Not to be used for diagnosis or treatment of illnesses.
◆This product improves the efficacy of rat sperm cryopreservation.
After thawing, rat sperm can be used for in vitro fertilization.

＊Reference(p９)：No.6【Manufacturer】

For use on rats only.

CARD Rat IVF Medium
CARD Rat IVF Medium is a novel medium
developed speciﬁcally for use in rat in vitro
fertilization. It oﬀers superior in vitro
ferdtilization rates, particularly when using
frozen-thawed rat sperm.

We can obtain high fertilization rates using
frozen-thawed sperm in several rats strains!

Frozen-thawed rat sperm

Fertilization rate（％）

100

95.8%

＊Reference(p９)：No.7

93.5% 90.7%

94.5%

60.2%

８０
６０

25.7%

４０
２０
０
CD (SD)

BN

LE

F344

Wistar

EGFP

P6

Reproductive Technology Tools for Mouse/Rat（A）

CARD Cold Transport Kit

（C）

（D）

The CARD Cold Transport Kit provides everything
you need to safely and cheaply transport mousecauda epididymides and 2-cell embryos at refrigerated
temperatures. The transported cauda epididymides
and embryos can used at the destination institution to
produce live mice via reproductive engineering
techniques.

（B）
Usage:
Transport of mouse cauda epididymides and2-cell embryos at refrigerated temperatures.
Cold storage retention period:
Max. 72 hours(when using our cold storage solution for cauda epididymides)
Kit contents:
A cold storage box (A), cold packs (B; 4 x large, 2 x small),a Thermos bottle (C), paper boxes and packing material (D).

CARD Rat Sperm Freezing Kit
CARD Rat Sperm Freezing Kit is a useful kit for producing
high-quality frozen rat sperm using CARD FERTIUP Rat
Sperm Cryopreservation Agent. It allows users to correctly
carry out the cooling process during the sperm

（A）

（B）
（C）

cryopreservation operation.

Kit contents:
A Styrofoam box (A), sperm cryopreservation straw
holding support (foam ﬂoat (B) and plate (C))

Embryo Manipulation Instrument Kit（D）

Our embryo manipulation instrument kit utilizes a glass capillary toensure the uniform transfer of embryos.

（C）

（B）

（E）

Set contents: Glass capillaries for embryo transfer (20 pieces; dry-heatsterilized) (A), a connector (B), a Pasteur pipette(C), a mouthpiece (D),a silicon tube (E)* We will send customers all equipment required for embryo transfer.

P7

（A）

Straw Connector
A straw connector is used for sucking
up sperm suspended in CARD FERTIUP
Sperm Cryopreservation Agent into
sperm straws.

Triangular Cassette Long (W278mm)
Triangular Cassette Short (W145mm)
Cassettes used when cryopreserving straws containing sperm
suspension in liquid nitrogen. We oﬀer two types of cassette: along 278mm cassette, which can store up to around 30 straws,
and a short 145mm, which can store up to around 15 straws.

Sperm Freezing Straws (10 pieces)This is a 0.25mL straw used for sperm storage.
Our straws enable the storage of sperm inliquid nitrogen for long periods of time. All
straws have been sterilized using EOG.

Freezing Canister
A freezing canister for use in sperm
cryopreservation. Using a ﬂoat guarantees
a cooling rate conducive to mouse sperm
cryopreservation on liquid nitrogen, which
in turn enables the storage of higher
quality frozen sperm.

P8

About Purchases from Outside of Japan
Our reproductive technology reagent products areused by research institutions around the world.

2792 Loker Avenue West, Suite 101
Carlsbad, CA 92010 USA
https://www.cosmobiousa.com/
info@cosmobiousa.com+1 858-334-3928

Technical support
If you have any questions, queries or issues concerning our reagents for use in mouse/rat
reproductive technology, please feel free to contact us using the contact details below.
Our veteran technicians are on hand to help solve any problems that you may have.

web_req@kyudo.co.jp

References
ReferenceNo.
1

cubation medium yields a high fertilization rate for cryopreserved C57BL/6J mouse sperm. Laboratory Animals, 44(2), 132-137, 2010

2

Takeo T, Hoshii T, Kondo Y, Toyodome H, Arima H, Yamamura K, Irie T, Nakagata N. Methyl-beta-cyclodextrin improves fertilizing
ability of C57BL/6 mouse sperm after freezing and thawing by facilitating cholesterol eﬄux from the cells. Biology of Reproduction, 78(3):546-51, 2008

3

Takeo T, Nakagata N. Reduced glutathione enhances fertility of frozen/thawed C57BL/6 mouse sperm after exposure to methyl-beta-cyclodextrin. Biology of Reproduction, 85(5), 1066-1072, 2011

4

Takeo T, Nakagata N. Immunotherapy using inhibin antiserum enhanced the eﬃcacy of equine chorionic gonadotropin on super-

5

Takeo T, Nakagata N. Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin

6

P9

Takeo T, Nakagata N. Combination medium of cryoprotective agents containing L-glutamine and methyl-β-cyclodextrin in a prein-

7

ovulation in major inbred and outbred mice strains. Theriogenology, 86(5), 1341-1346, 2016

increases the number of ovulated oocytes in C57BL/6 female mice. PLoS One, 10(5), 2015
Nakagata N, Mikoda N, Nakao S, Nakatsukasa E, Takeo T. Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats. Scientiﬁc Reports, 10, 93, 2020
Takeo T, Nakao S, Mikoda N, Yamaga K, Maeda R, Tsuchiyama S, Nakatsukasa E, Nakagata N. Optimized protocols for sperm
cryopreservation and in vitro fertilization in the rat. Lab animal, 51, 256-274, 2022

Products List
STANDARD

Storage Temp.

CARD FERTIUP Rat Sperm Cryoprotectant Agent

1.5 mL-80℃ to -20℃

CARD Rat IVF Medium

1.0 mL

４℃

STANDARD

Storage Temp.

Reagents for Rat Reproductive Technology

Reagents for Mouse Reproductive Technology
CARD FERTIUP Mouse Sperm Cryoprotectant Agent: CPA

CARD FERTIUP Mouse Sperm Preincubation Medium: PM
CARD MEDIUM Mouse Fertilization Medium
CARD FERTIUP PM (0.5mL) and
CARD MEDIUM Mouse Fertilization Medium Kit
CARD FERTIUP PM (1.0mL) and
CARD MEDIUM Mouse Fertilization Medium Kit
CARD HyperOva Superovulation Reagent for Mouse

CARD HyperOva F.D. Superovulation Reagent for Mouse
CARD ｍHTF Medium

CARD KSOMCARD 0.25M Sucrose
CARD 1M DMSOCARD DAP213

0.5 mL
1.0 mL
0.5 mL
1.0 mL
1 kit

18 to 28℃

4℃
4℃

1 set
4℃
1 set
0.6 mL
1.0 mL
1 kit
5 kit
2.0 mL
5.0 mL
2.0 mL
5.0 mL
2.0 mL
5.0 mL
1.0 mL
0.5 mL
1.0 mL-80℃ to -20℃

4℃

4℃

4℃

4℃
4℃
4℃

STANDARD

Storage Temp.

Straw Connector

1 unit-Triangular Cassette Short (W145mm)

10 units-

Triangular Cassette Long (W278mm)

10 units-

Sperm Freezing Straws (10 pieces)

10 units

-

1 kit

4℃*

Embryo Manipulation Instrument Kit

1 kit

-

Freezing Canister

1 unit-CARD Rat Sperm Freezing Kit

1 kit

-

Reproductive Technology Tools for Mouse/Rat

CARD Cold Transport Kit(*The storage temp. of cold storage solution for cauda epididymis)

Mouse/Rat Reproductive Engineering Reagents are intended for research use only and are not intended for diagnostic or therapeutic purposes.
FERTIUP, CARD MEDIUM, and CARD HyperOva are registered trademarks.
All information is current as of October 2022.

P10

Are you having trouble
storing your transgenic
mouse or rat strains?
Are you struggling withmaintenance, upkeep or
breeding?
We can help.
We at Kyudo Co, Ltd. provide a wide range of reagents foruse at every stage of mouse and rat reproductive technology,
from superovulation and in vitro fertilization through to thefreezing of sperm and embryos.

These high quality reagents were developed in collaboration
with the Center for Animal Resources and Development,
Kumamoto University. We manufacture and sell the reagents
under license to customers worldwide.

Using reproductive engineering techniques alongside ourreagents contributes to a signiﬁcant reduction in the numberof animals required per experiment, thereby following
the 3R Principle.

Kyudo Co., Ltd

Frequently Asked Questions (FAQ)
General Questions
Q1

Is it possible to download the online manual for reproductive engineering-related reagents?

A1

You can download it from our homepage. Please refer to the URL below.
https://www.cosmobiousa.com/content/document/cosmo-bio-ltd/10167_KYD_CARDmanual_english.pdf

Q2

Do the products contain substances harmful to the human body?

A2

No, the products do not contain substances harmful to the human body.

Q3

Are SDS (Safety Data Sheet) available for the products?

A3

Yes, SDS (Safety Data Sheet) is available for each reproductive engineering-related reagent product. Please
send us a request by e-mail (info@cosmobiousa.com)

Q4

Can the products be used for therapeutic purposes in humans or animals?

A4No, our reproductive engineering reagents are intended for research use only. They are not intended for diagnostic or therapeutic use.

Q5

There is a foreign object in the product, can I exchange it?

A5Yes, please send us an inquiry with the photo of the condition and we will replace them.

Q6

After using the product, is it possible to dispense the remaining amount and store it at low temperature(freeze, refrigerate)?

A6

We cannot guarantee the quality after cold storage, and do not recommend storage at low temperatures.

Q7

Please tell me how to store mouse and rat reproductive engineering-related reagents and expiration dates.

A7[Preservation method and expiration date]
Please use by the indicated expiration date
1. Reproductive engineering-related reagents for mice
CARD FERTIUP mouse sperm cryopreservation medium: room temperature (18-28°C), 18 months after
production date
CARD FERTIUP mouse sperm preculture medium: refrigerated (2-10°C), 18 months after production date
CARD MEDIUM ® : Refrigerate (2-10°C), 18 months after production date
CARD HyperOva ® : Frozen (-80°C), 36 months after production date
CARD HyperOva ® FD: Refrigerate (4°C), 24 months after production date
2. Reproductive engineering-related reagents for rats
CARD FERTIUP Rat Sperm Cryopreservation Solution: Frozen (-20°C or below), 24 months after production date
CARD Rat IVF Medium: Refrigerated (4°C), 18 months after production date

Q8

Can reproductive engineering-related media be used immediately after delivery?

A8

Filter sterilization is not required unless specified in the usage instructions. Please use it after gas equilibrium
etc. according to the usage of each product.

Frequently Asked Questions (FAQ)
CARD FERTIUP® and CARD MEDIUM® (Mouse)
Questions regarding the CARD FERTIUP® Products
Q1

We were able to obtain a high fertility rate for the C57BL/6 strain of mouse via in vitro fertilization using cryopreserved spermatozoa treated with CARD FERTIUP® Mouse Cryoprotectant/Mouse Preincubation Medium.
Does the media increase the in vitro fertility rate of other strains too?

A1

When mouse sperm cryopreservation medium/mouse sperm pre-incubation medium is used together, it iseffective in ameliorating the fertility rates in many commercially available mouse strains.

Q2

After opening the product, can it be subdivided and frozen?

A2

We cannot guarantee the quality after freezing, so we do not recommend this method of storage.

Q3

Can I use left over CARD FERTIUP® Mouse Cryoprotectant if I transfer the medium into tubes and store it atrefrigerated temperatures?

A3

Once opened, use up the ampoule as quickly as possible. Please purchase an ampoule size which you can use
up in one occasion. Please note that the cryoprotectant ampoule should be stored at room temperature as it
contains high doses of saccharides and skim milk, which may sink to the bottom of the ampoule after refrigerating or freezing.

Q4

There are crystals/precipitants in my un-opened CARD FERTIUP® Mouse Cryoprotectant.

A4

In rare cases, crystals may precipitate due to changes in ambient temperature (low temperature).
Incubate at 37°C for 30 minutes to 1 hour, and stir to melt the crystals. There is no problem with cryopreservation performance after thawing.

Q5

Liquid paraffin is used in the usage instructions for the CARD FERTIUP® series; doesn’t the paraffin have adverse effects on sperm?

A5

Liquid paraffin does not adversely affect sperm suspension (sperm suspended in storage solution).

Q6

After preincubating cryopreserved sperm with CARD FERTIUP® Mouse Preincubation Medium, what is thesperm concentration when carrying out in vitro fertilization using an IVF medium?

A6

Typically, the sperm concentration in cryopreserved sperm ranges from 100,000~200,000 sperm/µL. Whenintroducing freeze-thawed sperm suspension (10 µL) into Mouse Sperm Preincubation Medium (90 µL), thesperm concentration is diluted tenfold. This means that the sperm concentration in the preincubation medium is 10,000~20,000 sperm/µL. Thereafter, the preincubated sperm suspension (10 µL) is further diluted tenfold when it is introduced into IVF medium (90 µL). This results in a sperm concentration in the IVF mediumof 1,000~2,000 sperm/µL. Finally, the ratio of motile sperm in after freeze-thawing is around 20%, whichmeans that the motile sperm concentration in the IVF medium is 200~400 sperm/µL.

Q7

I would like to download the instruction manual for the CARD FERTIUP® series.
Where can I download them from?

A7

They are available on our website,
https://www.cosmobiousa.com/content/document/cosmo-bio-ltd/10167_KYD_CARDmanual_english.pdf

Q8

Is there a method to confirm whether the freezing and thawing of sperm has been correctly carried out?

A8

1. To confirm the quality of sperm after freeze-thawing, preincubate the sperm in preincubation medium
after freezing and judge according to the number of active motile sperm at the edge of the drop.
2. For final confirmation, carry out in vitro fertilization of unfertilized oocytes and confirm that you haveattained at least a 30% fertility rate. When checking the motility of the sperm before freezing, dilute the suspension of sperm in medium such as CARD mHTF or PB1 medium.
(We carry out IVF during lot checks to check and confirm that all of our products on sale attain an in vitro fertility rate of 30% or above for C57BL/6 mice)

Q9

How about legacy stock of cryopreserved spermatozoa?

A9Please Check here. Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa

Q10

In vitro fertilization does not work as well as we thought. Sperm and eggs have no problem but the embryos
died the next day. What kind of problems can be considered?

A10

The quality of liquid paraffin could be a problem that effects in vitro fertilization rates, and arresting embryogenesis . Moreover, liquid paraffin gradually degrades after opened. Please use as promptly as possible after
opening. We recommend using the following products.
Manufacturer name: Nacalai Tesque
Grade: Specially Prepared Reagent for tissue culture
Name of product: Paraffin liquid
Model number: 26137-85

Questions regarding CARD Medium®
Q1

For how many months after the manufacture date is CARD MEDIUM® warranted for?

A1

The warrantee period for CARD MEDIUM® is 18 months. This warrantee period may increase in the future,depending on the results of investigations and reviews.

Q2

Is it possible to attain a high in vitro fertility rate without using CARD MEDIUM® with CARD FERTIUP® series?

A2

No. The CARD FERTIUP® series works with sperm and CARD MEDIUM® with oocytes to raise the fertility
rate. Use both products simultaneously to attain a fertility rate of 80% or more even with C57BL/6 strain
mouse.

Q3

Can high in vitro fertilization rates be obtained with strains other than C57BL/6 mice?

A3

Yes, high in vitro fertilization rates can be obtained in various mouse strains.
[Reference link] DOI: 10.1016/j.theriogenology.2016.04.076(Link: https://www.sciencedirect.com/science/article/abs/pii/S0093691X16301194?via%3Dihub)

Q4

In vitro fertilization was performed with CARD MEDIUM ® , and embryonic development beyond the 2-cellstage did not progress well. The medium used for in vitro culture is KSOM medium or Whitten medium. What problems could have slow down the development of the embryo?

A4

The following problems are possible.
1. The incubator temperature and carbon dioxide gas concentration
2. The in vitro culture medium; If prepared by yourself, there may be problems with the distilled water or
ingredients. Please consider using "CARD KSOM".
3. The mineral oil that coats the medium: If old, the embryo development may be inhibited.

Frequently Asked Questions (FAQ)
CARD FERTIUP® Rat Sperm Cryopreservation Agentand CARD Rat IVF MEDIUM
General Questions regarding CARD FERTIUP® Rat Sperm Cryopreservation
Agents
Q1

How should I store CARD FERTIUP® Rat Sperm Cryopreservation Agent? By when should I use it?

A1

1. Please store CARD FERTIUP® Rat Sperm Cryopreservation Agent in a freezer at or below -20°C.
2. Use before the best before end usage date which is two years (24 months) from the manufacture date.

Q2

After opening a vial of CARD FERTIUP® Rat Sperm Cryopreservation Agent, can I aliquot the contents into
smaller portions and use it for cryopreservation?

A2

No. CARD FERTIUP® Rat Sperm Cryopreservation Agent is sold in 1.5mL measures, and one vial (1.5mL) is to
be used per rat cauda epididymis. Please follow the usage instructions and use one vial (1.5mL) of CARD FERTIUP® Rat Sperm Cryopreservation Agent per rat cauda epididymis.

Q3

Does CARD FERTIUP® Rat Sperm Cryopreservation Agent contain any ingredients which are harmful to humans?

A3

No. It does not contain ingredients which are harmful to humans.

Q4

There appears to be a foreign object in my vial of CARD FERTIUP® Rat Sperm Cryopreservation Agent.
Can I get a replacement?

A4

Kyudo carries out thorough quality control; however, if you have contamination in your vial of CARD FERTIUP® Rat Sperm Cryopreservation Agent, we would be happy to replace your vial with a new one.

General Questions regarding CARD Rat IVF Medium
Q1

How should I store CARD Rat IVF medium? By when should I use it?

A1

1. Please store CARD Rat IVF medium in a refrigerator at 4°C.
2. Use before the best before end usage date of CARD Rat IVF medium is 1.5 years (18 months) from the manufacture date.

Q2

After opening a vial of CARD Rat IVF medium, can I aliquot into smaller portions and use it for cryopreservation?

A2

No. After freezing, we cannot guarantee the quality of the sperm.

Q3

Does CARD Rat IVF medium contain any ingredients which are harmful to humans?

A3

No. It does not contain any ingredients which are harmful to humans.

Q4

There appears to be a foreign object in my vial of CARD Rat IVF medium. Can I get a replacement?

A4

Kyudo carries out thorough quality control; however, if your vial is contaminated upon receival, we certainly
will replace your vial with a new one.

Frequently Asked Questions (FAQ)
CARD HyperOva® and CARD HyperOva® FD
Questions regarding CARD Hyperova®
Q1

After opening the CARD HyperOva®, can it be subdivided and stored frozen?

A1

No. We do not recommend refreezing after thawing because we cannot guarantee the quality.

Q2

I didn't ovulate much. Is it defective?

A2

For C57BL/6J mice, administration is recommended between 26 and 30 days of age (birth date is considered
as 0 days of age). Due to the nature of the product, the effects of this product may not be fully obtained atcertain ages. In addition, the optimum age in weeks may differ depending on the strain. For special strains
and ages, please contact us individually.

Q3

A sediment is deposited on the product. Are there any problems with using it?

A3

In rare cases, ingredients may precipitate. Precipitates are not the main component of this product. Avoid
deposits during use.

Q4

Is CARD HyperOva ® effective against hyperovulation in rats?

A4

This product is manufactured based on mouse antigens. The effect of superovulation in rats has been confirmed to some extent, though not as much as in mice. In the case of rats, we administer 0.4-0.8mL up to 5week-old rats, and 48-56 hours later, hCG (15-30IU/head) is administered, and eggs are collected the nextmorning. Please note that the reactivity differs depending on the rat strain

Q5

Is CARD HyperOva® a mixture of equine CG and goat anti-inhibin antiserum?

A5

Yes, it is mixed.

Q6

Tell me more about the horse gonadotropin.

A6

The horse gonadotropin in CARD HyperOva® is purified from a horse born and raised in Japan.

Q7

What is the country of origin for the goat?

A7

The goat is a male Saanen goat born and raised in Japan.

Q8

Did the goat co-mingle with any other livestock?

A8

No. Only with other goats.

Q9Is the antibody unprocessed goat serum?

A9

The goat serum is diluted 2x with physiological saline. The horse gonadotropin is also dissolved in saline.

Q10

For the goat anti-mouse inhibin antiserum, what is the source of the immunogen?

A10

The immunogen is a synthetic peptide.

Q11

I can see a clump/precipitate/sediment in the vial.

Q12

Such imperfections do not diminish the effectiveness of CARD HyperOva®. Please avoid the clumps and use
only the solution.

Q13

Can the product in 1ml vials be refrozen after being thawed if we don't need all of it at the moment?

A13

Once the 1ml vial is opened, please use it to administrate intraperitoneally all at once. 0.1mL - 0.2 mL can beadministered to each mouse.

Q14

Will the product work after storage at 4°C for one week instead of -20°C ?

A14

We cannot guarantee the quality under this storage condition. The CARD HyperOva may lost activity and wecannot recommend using it.

Frequently Asked Questions (FAQ)
CARD HyperOva® and CARD HyperOva® FD
Questions regarding CARD Hyperova® FD
Q1

Does the CARD HyperOva ® FD have an expiration date?

A1

Yes. As of January 2020, it is two years from the manufacture date. Please use it within the expiration date.

Q2

What is the difference between conventional CARD HyperOva ® and CARD HyperOva ® FD?

A2

CARD HyperOva ® FD is manufactured with a new formulation suitable for lyophilization. Conventional products needed to be stored at -20°C to -80°C, but this product can be stored at 4°C.

Q3

Are there any difference in the superovulation performance of CARD HyperOva ® FD compared to conventional products?

A3

Please expect the same superovulation results as the conventional CARD HyperOva ®

Q4

What should I do for long-term storage after thawing CARD HyperOva ® FD?

A4

Aliquot and store at or below -20°C. Please thaw before use. The superovulation effect has been confirmed
to be maintained for about 3 months after the product is thawed or frozen.

Frequently Asked Questions (FAQ)
Others
Q1

Frozen mouse sperm has been sent from the United States, and we are considering in vitro fertilization. Is it
possible to perform in vitro fertilization using cryopreserved mouse sperm frozen in a method other than the
CARD method?

A1

For in vitro fertilization methods using sperm frozen by methods other than the CARD method, please refer tothe online manual of the Kumamoto University Life Resource Research and Support Center (CARD).

Q2

IVF didn't go as well as expected. The sperm and egg are fine, but the embryo dies. What are the possible
causes?
The quality of liquid paraffin is one of the reasons why IVF fails and embryos stop developing. We recommend
using the following liquid paraffin products. In addition, we have experienced that the quality of liquid paraffin deteriorates after opening. Please use as soon as possible after opening.

A2

Manufacturer name: Nacalai TesqueStandards: Special reagent for tissue culture
Product name: Liquid paraffin
Model number: 26137-85

AVAILABLE FOR DOWNLOAD

in English, French and Spanish

Reproductive Engineering Techniques in Mice
Technical Manual
by Naomi Nakagata (3rd Edition)

https://www.cosmobiousa.com/content/document/cosmo-bio-ltd/10167_KYD_CARDmanual_english.pdf

Currently in a its 3rd Edition, a limited number ofprinted books are available from Cosmo Bio USA.
Please contact us for details (info@cosmobiousa.com).
REPRODUCTIVE ENGINEERING TECHNIQUES IN MICE
Copyright©2015 Naomi Nakagata
ALL RIGHTS RESERVED. PRINTED IN JAPAN.
This book is not to be sold.

Developed
Developed
andandManufactured
Manufactured
by byInternational
International
Distribution
Distribution
PartnerPartnerTThhee44M
MID
ID®®KKititss::FFuunnccttioionnaal lAAssssaayyss
ooffSSppeerrm
mQQuuaaliltityyaannddM
MaaleleFFeerrttiliiltityy

Sperm
Spermquality
quality
Fertility
Fertility

Cryopreservation
Cryopreservationwww.4biodx-breeding.com
www.4biodx-breeding.com

PRODUCT LIST

• Innovative Sperm Quality Biomarkers •Reference

Designation

Specificity

Price

4VDX-18K2

Pig 4MID® Kit

Pig proAKAP4

request a quote

4VDX-18K3

Horse 4MID® Kit

Horse proAKAP4

request a quote

4VDX-18K4

Bull 4MID® Kit

Bovine proAKAP4

request a quote

4VDX-18K5

Dog 4MID® Kit

Dog proAKAP4

request a quote

4VDX-18K6

Rabbit 4MID® Kit

Rabbit proAKAP4

request a quote

4VDX-18K7

Ram 4MID® Kit

Ram proAKAP4

request a quote

4BDX-18K8

Mouse 4MID® Kit

Mouse / Rat proAKAP4

request a quote

4VDX-18K9Goat 4MID® Kit

Goat proAKAP4

request a quote

4VDX-19K10

Cat 4MID® Kit

Cat proAKAP4

request a quote

4VDX-19K11

Camel 4MID® Kit

Camel proAKAP4

request a quote

Reference

Antibody

Reactivity

Price

4BDX-1901

Anti-proAKAP4(clone 5H2) - 100 µg

Goat, Camel, Cat, Dog, Pig,
Rabbitrequest a quote

4BDX-1901S

Anti-proAKAP4(clone 5H2) - 20 µg

Goat, Camel, Cat, Dog, Pig,
Rabbitrequest a quote

4BDX-1701

Anti-proAKAP4(clone 6F12) - 100 µg

Horse, Bull, Ram, Camel, Mouse

request a quote

4BDX-1701S

Anti-proAKAP4(clone 6F12) - 20 µg

Horse, Bull, Ram, Camel, Mouse

request a quote

4BDX-1602

Anti-AKAP4(clone 7E10) - 100 µg

Horse, Bull, Mouse, Rat, Camelrequest a quote

4BDX-1602S

Anti-AKAP4(clone 7E10) - 20 µg

Horse, Bull, Mouse, Rat, Camelrequest a quote

4BDX-1805

Anti-AKAP4(clone 10F8) - 100 µg

Dog, Rabbit, Pig, Ram, Cat, Goat

request a quote

4BDX-1805S

Anti-AKAP4(clone 10F8) - 20 µg

Dog, Rabbit, Pig, Ram, Cat, Goat

request a quote

Cat 4MID® Kit
Cat proAKAP4
request a quote
4VDX-19K10
All our monoclonal antibodies are validated for Western-Blot, Immunohistochemistry, Immunofluorescence andCamel 4MID® Kit
Camel proAKAP4
request a quote
4VDX-19K11
Flow cytometry
applications.

We sell 4BioDx products directly or through our distributor Cosmo Bio USA. Please contact us atcontact@4biodx.com or contact them at info@cosmobiousa.com
All our products are sold for in vitro use only, not to be administered to humans or animals andnot to be used as diagnostics nor therapeutics.

V1_1121

4MID® Kits : Anti-proAKAP4 ELISA kits

Cosmo Bio Brand Media and Solutions for RODENT and PIG IVF-Related Procedures

DAP213 (DMSO, Acetamide, Propylene Glycol)
DMSO 1M
HTF (Human Tubal Fluid)
M2
Modified Krebs-Ringer Bicarbonate (mKRB) medium
Mouse KSOM (Potassium Simplex Optimized Medium)mR1ECM (modified Rat 1-cell Embryo Culture Medium)mWM (modified Whitten’s Medium)P10 (Propylene Glycol in PB1)PB1PEPeS (Percoll in PB1)
R18S3 (Medium for Mouse Sperm Cryopreservation)
Rat KSOM (Potassium Simplex Optimized Medium)
SPB1
Sucrose 0.25M

Basic Culture Kit for Porcine IVF Embryo Production
Complete Culture Kit Porcine IVF Embryo Production
POE-CM (Porcine Oocyte/Embryo Collection Medium)
POM (Basic Medium For Porcine Oocyte Maturation)
HP-POM (High Performance Porcine Oocyte Maturation Medium)dbcAMP-100X (dbcAMP Concentrate, 100x Solution)
SEM-5X (Porcine Semen Dilution Medium)
PFM (Porcine Fertilization Medium): CSR-CK023
PZM-5 (Porcine Embryo Development Medium)
PBM (Porcine Blastocyst Medium)

Cosmo Bio USA, Carlsbad, CA 92010+1 760-431-4600

SizeCat. No.

10 x 1 ml
10 x 2 ml
10 x 2 ml / 10 x 5 ml
10 x 2 ml / 10 x 5 ml
100 ml10 x 2 ml / 10 x 5 ml
10 x 2 ml / 10 x 5 ml
10 x 2 ml / 10 x 5 ml
10 x 2 ml
10 x 2 ml / 10 x 5 ml
10 x 1 ml
10 x 1 ml
10 x 1 ml
10 x 2 ml / 10 x 5 ml
10 x 2 ml / 10 x 5 ml

CSR-R-T073
CSR-R-T072
CSR-R-B070 / CSR-R-B071
CSR-R-M083 / CSR-R-M084
CSR-R-M193
CSR-R-B074 /CSR-R-B075
CSR-R-M191 / CSR-R-M174
CSR-R-B080 / CSR-B081
CSR-R-P186
CSR-R-P185 / CSR-R-P138
CSR-R-P187
CSR-R-R082
CSR-R-R148
CSR-R-S187 / CSR-R-S188
CSR-R-Y077 / CSE-R-Y078

Size

Cat. No.

1 kit
1 kit
5 x 100 ml
3 x 25 ml
3 x 25 ml
500 ul25 ml
2 x 100 ml
3 x 25 ml
2 x 25 ml

CSR-CK029
CSR-CK030
CSR-CK020
CSR-CK021
CSR-CK022
CSR-CK027
CSR-CK026
CSR-CK023
CSR-CK024
CSR-CK025

www.cosmobiousa.com

info@cosmobiousa.com