Instructions to authors for the Journal of Electrophoresis
The Journal of Electrophoresis is the official and international journal of the Japanese Electrophoresis
Society. It is written in English, published under the responsibility of the Editorial Board of the JapaneseElectrophoresis Society, and supports the development of electrophoretic technologies and theirapplications to biochemical or biomedical research and clinical investigations. Topics include new or
improved analytical and preparative methods, theory development, clinical observations, and innovative
applications of electrophoresis and its related separation and detection methods in the study of nucleic
acids, proteins, carbohydrates, lipids, and many other biomolecules and clinical samples. The Journalof Electrophoresis is an open access journal, and all papers published in it are freely available to anyone
for reading and downloading via J-STAGE, an electronic journal platform for science and technology
information in Japan (https://www.jstage.jst.go.jp/browse/jelectroph).

1.

Types of papers

The types of papers published are classified as follows:1)

Full Papers: full-length papers that are original and novel, containing worthy facts or conclusions.

2)

Short Communications: short papers containing new facts or a worthy conclusion based on partsof a research.

3)

Reviews: papers that comprehensively and systematically discuss a trend in electrophoresis
research and its related fields and papers that newly summarize and systematize the results ofseveral papers on a specific topic that an author has previously published.

4)

Technical Notes: papers reporting findings or data concerning the development or improvementof techniques, methods, equipment, reagents, and protocols, among others.

5)

Case Reports: papers reporting clinical observations that are of particular interest due to theirnovelty and potential impact on biomedical research and clinical practice.

All types of papers will be peer-reviewed according to the criteria of originality and quality by theEditorial Board of the Japanese Electrophoresis Society.

2.

Manuscript preparation

Manuscript preparation should adhere to the following guidelines:

1) Manuscripts should be prepared as Microsoft Word files and typed in an 11-point font with double
spacing throughout, including references, tables, and figure legends, on a white page 210 × 295mm (ISO A4) with at least 25-mm margins all around (25–28 lines per page). Insert serial page
numbers and serial line numbers on the manuscript pages. Manuscripts should be written in
English throughout. Use Arabic numerals when referring to figures and tables. Units should be
expressed as SI units (m, kg, s, A, K, mol, Hz, V, °C, etc.) and non-SI units (min, h, d, L, etc.)
officially accepted for use with the SI.

2) Manuscripts should comprise the following sections: i) title page, ii) text, iii) tables, iv) figure1legends, and v) figures. Tables and figures are not essential and need not be included dependingon the manuscript type.

3) Title page: Each manuscript should have a separate title page. The structure of the title page is asfollows: i) type of paper (Full Paper, Short Communication, Review, Technical Note, or CaseReport), ii) article title, iii) authors’ names [write given name(s) first and then family name(s),with initial capital letters], iv) authors’ affiliations [if the coauthors are not from the same
institution, place superscript Arabic numbers (1, 2, 3, etc.) immediately after the appropriate
author’s name and in front of the appropriate institution], v) name and complete postal address,e-mail address, and phone/fax number of the corresponding author, which should be marked withan asterisk (*), and vi) 3–5 key words or key phrases appropriately expressing the content of thetext. If necessary, vii) abbreviations used should also be listed.

4) Text: The text for Full Papers and Short Communications should contain the section headings ofSUMMARY (less than 250 words), INTRODUCTION, MATERIALS AND METHODS,RESULTS,DISCUSSION,andREFERENCES.

Ifrequired,CONCLUSIONS

andACKNOWLEDGMENTS sections should be added immediately before the REFERENCESsection. The RESULTS and DISCUSSION sections may be combined into a RESULTS ANDDISCUSSION section, which should then be followed by a CONCLUSIONS section. Reviews
do not require MATERIALS AND METHODS, RESULTS, or DISCUSSION sections but must
contain SUMMARY (less than 250 words), INTRODUCTION, CONCLUSIONS, andREFERENCES sections. Technical Notes and Case Reports should not be divided into titledsections and should be written in a continuous style. If required, a REFERENCES section can be
added at the end of the manuscript.

5) Text word count: The text should not exceed the following number of words, according to themanuscript type:・ Full Papers: 6,500 words
・ Short Communications: 3,000 words
・ Reviews: 8,500 words
・ Technical Notes and Case Reports: 2,500 words
All word counts include the REFERENCES section as well as figure and table legends. Tables
and Figures should be counted as 200 words per table or figure and included in the word count.
Papers exceeding the word limits described above may only be published if approved by theEditorial Board.

6) Subsections can be included in the MATERIALS AND METHODS, RESULTS, andDISCUSSION sections. However, the SUMMARY, INTRODUCTION, and CONCLUSIONS
sections should not include subsections. In the SUMMARY section, no line breaks should beincluded.

7) The names of chemical compounds used should be according to the IUPAC nomenclature.
However, element symbols and chemical formulas of simple inorganic compounds can be used.

28) Abbreviations must be defined at first use in the text, with the abbreviation written in parentheses
following the full term. Standard abbreviations that can be used in the title, summary, or text
without definition are listed in Appendix Table 1.

9) Company names should be described with the name of the city and the country where thecompany is located.

10) For in-text citations, cite only the family name of the first author of the reference (withoutprefixing titles) followed by “et al.” instead of the names of the coauthor(s).

11) Refer to figures and tables in the text as Fig. 1, Table 1, etc.
12) Literature references should be numbered consecutively in order of their first citation in the text,
and the corresponding reference list numbers should be placed at the appropriate locations as
superscript numerals 1,2) or 3-5) when three or more references are cited.

13) Literature references should be described in the REFERENCES section at the end of the text.
Citation style should follow Citing medicine: the National Library of Medicine style guide forauthors, editors, and publishers (2nd Edition) (https://www.ncbi.nlm.nih.gov/books/NBK7256/),
published by the National Library of Medicine.
Journalexample:Namesofallauthors. Articletitle.

Journaltitle. Yearofpublication;volume:page range (print journals) or article number (on-line journals).
1) Kinoshita E, Kinoshita-Kikuta E, Koike T. Separation and detection of large
phosphoproteins using Phos-tag SDS-PAGE. Nat Protocol. 2009;4:1513–1521.
If there are four or more authors, mention the names of the first three authors followed by “etal.”
2) Kinoshita E, Kinoshita-Kikuta E, Takiyama K, et al. Phosphate-binding tag, a new tool tovisualize phosphorylated proteins. Mol Cell Proteomics. 2006;5:749–757.
3) Kameyama A, Dissanayake SK, Thet Tin WW. Rapid chemical de-N-glycosylation andderivatization for liquid chromatography of immunoglobulin N-linked glycans. PLoS One.
2018;13:e0196800.
Books example: Names of all authors. Title. Names of all editors. Name of book (volume andedition). Place of publication: Publisher; year of publication. Page number/page range.
3)

Kinoshita-Kikuta E, Kinoshita E, Koike T. Phos-tag technology for kinomics. In: Kraatz
H-B, Martic S, editors. Kinomics: Approaches and applications. Weinheim: Wiley-VCH
Verlag GmbH & Co. KGaA; 2015. p. 195–210.

14) Tables: Tables should be prepared in English and separated from the text. Do not use vertical lines
in tables. Titles should be placed at the top and brief descriptions at the bottom of the tables.

15) Figure legends: Figure legends should be prepared in English and placed together at the end ofthe manuscript before the figures, separately from the text and figures. If figures are reproduced
from another source, the authors must obtain permission from the copyright holder to reproduce
them and acknowledge the original source as follows:Fig. 1 Schematic diagram of analytical system3

1: DW; 2: EtOH; 3: MeOH; 4: DMSO. Reprinted with permission from Ref. 16 © (2014) WileyVCH Verlag GmbH & Co. KGaA, Weinheim.
The Japanese Electrophoresis Society shall not be held liable for damages caused by, for example,
figure reproduction by the authors without prior permission.

16) Figures: Authors may be asked to redraw figures that are unsuitable to online publication. Figures
should be prepared in English and placed on A4 paper with one figure per sheet. The number ofthe figure and name of the first author should be written to the lower left of the figure. Coloredfigures are welcome.

17) Image manipulation is unacceptable. All figures must accurately reflect the original data.
Information should not be enhanced, eliminated, added, obscured, or moved. In cases where
manipulation is unavoidable, this should be clearly detailed in the figure legends. All instruments,software, and processes used to obtain the images must be fully detailed in the manuscript, either
in the MATERIALS AND METHODS section or the figure legends. Acceptable image
manipulation includes uniformly adjusting the contrast of an entire image and any control images
and ensuring that all original data, including the background, remains visible and that no newfeatures are introduced. The cropping of gels or repositioning of lanes/fields is permitted
providing that all alterations are clearly indicated by dividing lines in the image itself, vital data
are not removed, and an explanation of the alterations is included in the figure legend.
Unacceptable manipulation includes, but is not limited to, the enhancement of one feature/bandover others and the removal of background noise/bands, etc. Authors must be able to produce alldata in its raw format upon editorial request.

3.

Important manuscript submission requirements and notices

The authors should pay attention to the following points concerning manuscript submission:

1) The work mentioned in the manuscript must comply with the Declaration of Helsinki, theFundamental Guidelines for Proper Conduct of Animal Experiment and Related Activities in
Academic Research Institutions (Ministry of Education, Culture, Sports, Science and Technology,Japan, Notice No. 71, June 1, 2006, https://jes1950.jp/Download2/hoi1-10th-E-monkashou.pdf),
and any guidelines and standards for experiments and research established by related institutions,facilities, or academic societies.

2) Information regarding the conflict of interest in the work mentioned in the manuscript or the lackthereof should be disclosed and noted before the REFERENCES section.

3) In cases where the work involves issues relevant to human rights protection and legal compliancethat require obtaining the consent and/or cooperation of a third party, consideration in the handlingof personal information or actions related to bioethics and/or biosafety (including the laws,regulations, and guidelines in the country/region(s) where the work is to be conducted) and the4measures and actions for these issues should be disclosed and noted in the manuscript.

4) Do not submit a paper describing essentially the same work to other journals before the decisionof acceptance or rejection has been received. A paper under submission for publication elsewhere
cannot be received.

5) Manuscripts are accepted from both members and non-members of the Japanese Electrophoresis
Society.

6) An article submission charge of JPY 50,000 is payable by the authors. However, the charge iswaived if the first or corresponding author is a member (regular or associate member only) of theJapanese Electrophoresis Society or if the Editorial Board requested the paper.

7) The manuscript, including the i) title page, ii) text, iii) tables, iv) figure legends, and v) figures(tables and figures are not essential and need not be included depending on the manuscript type)should be submitted to the journal’s Editorial Office (den-ei@nacos.com) via e-mail in the formof a single PDF file less than 10 MB in size. If the file exceeds 10 MB, please consult with theEditorial Office beforehand. The mailing address and phone/fax numbers of the Editorial Office
are as follows:
The Editorial Office of the Journal of Electrophoresis
c/o: Nakanishi Printing Company Limited
Shimotachiuri, Ogawa Higashi, Kamikyo-ku, Kyoto 602-8048, Japan
Phone: +81 75 441 3155, Fax: +81 75 417 2050, E-mail: den-ei@nacos.com

8) Supporting information is encouraged in the form of supplementary materials such as data sets,figures, movie files, audio clips, 3D structures, and other related nonessential multimedia files.
Supplementary materials should be cited within the manuscript text (refer to supplementaryfigures or tables in the text as Supplementary Fig. 1, Supplementary Table 1, etc.), and a
descriptive legend should be included. All supplementary materials will be included in the peerreview process. Thus, the materials must be submitted to the journal’s Editorial Office via e-mail(den-ei@nacos.com) with the main body of the manuscript but as separate files. Supplementarymaterials are published online as supplied by the author, and a proof is not made available prior
to publication. Thus, authors should provide any supplementary materials in the desired final
format.

9) A received date and a receipt number for the manuscript will be sent to the corresponding author
via e-mail.

10) If there are questions pertaining to the writing, format, submission, or sending of manuscripts orif there is no reply within 10 days after manuscript submission, please contact the journal’sEditorial Office mentioned above.

4. Review of submitted papers
1) Manuscripts are reviewed by the Editorial Board in accordance with the journal regulations, and5

the Editor-in-Chief will inform the author of the results of the review.

2) The Editor-in-Chief will inform the author if the manuscript is accepted for publication. The datewhen such information is relayed will be recorded as the acceptance date.

3) If the manuscript is accepted, the final version of the manuscript (prepared using Microsoft Word)
and figures (color: 300 dpi, grayscale or line drawing: 600 dpi) should be submitted in electronic
format to the Editorial Office mentioned above.

5. Page proofs and correction of errors
1) As a general rule, the author performs the first proof prior to online publication, and the Editorial
Board the second and subsequent proofs.

2) The author can only make typographic corrections at the first proof stage.
3) If the author requests corrections within 6 months following online publication, they will betreated as follows (all correction requests by the author after 7 months will not be accepted):a) Typographic errors will be published as a correction notice.
b) Any corrections and additions other than typographic errors will not be accepted.

6. Copyright, publication fees, and other issues
1) The copyright of the paper to be published in the Journal of Electrophoresis belongs to theJapanese Electrophoresis Society.

2) If the content of a paper, including figures and tables, will be reproduced, reused, or modified inother publications after the decision to publish the paper in the Journal of Electrophoresis, pleaseask permission from the Editorial Office mentioned above beforehand. Reproduction, reuse, ormodification will be permitted upon the approval of the Editor-in-Chief. However, publication inother media before publication in the Journal of Electrophoresis will not be permitted.

3) Expenses for editing and publication, including publication fees, of accepted papers are notcharged. Authors who would like to order hardcopy reprints can do so at the author proof stage.
The reprint expenses will be charged according to the number of copies (minimum of 50 copies,in multiples of 10 copies) that the author requests.

6Appendix Table 1. Standard abbreviations that can be used in the title, summary, or text.
BisN, N'-methylenebisacrylamidebpbase pairs%C

cross-linking agent (g/100 mL)/%TCBB

Coomassie brilliant blue

cpm

counts per minute1D

one-dimensional

2D

two-dimensional

DaDalton (molecular mass)

2DE

two-dimensional gel electrophoresis

DIGE

fluorescence difference gel electrophoresis

DGGE

denaturing gradient gel electrophoresis

DMEM

Dulbecco’s modified Eagle medium

DMSO

dimethyl sulfoxideDNAdeoxyribonucleic acid

DTT

dithiothreitol

ECL

enhanced chemiluminescence

EDTA

ethylenediaminetetraacetic acid

EGTA

ethylene glycol-bis(β-aminoethylether)-N,N,N',N'-tetraacetic acid

ELISA

enzyme-linked immunosorbent assay

GST

glutathione S-transferase

HEhematoxylin and eosin

HEPES

N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)HPLC

high-performance liquid chromatography

IEF

isoelectric focusing

Ig

immunoglobulin

IPimmunoprecipitation

IPG

immobilized pH gradient

IPTG

isopropyl-β-D-thiogalactopyranosidekbp

kilobase pairskDakilodalton (molecular mass)

LCliquid chromatography

MALDI-MSmatrix-assisted laser desorption/ionization-mass spectrometry

Mbp

megabase pairs

MES

2-(N-morpholino)ethanesulfonic acid

MOPS

3-(N-morpholino)propanesulfonic acidMr

relative molecular mass (dimensionless)MS

mass spectrometry

7MS/MStandem mass spectrometrym/z

mass-to-charge ratio

NMR

nuclear magnetic resonance

NP-40

Nonidet P-40

OD

optical density

ORF

open reading frame

PAGE

polyacrylamide gel electrophoresis

PBS

phosphate-buffered saline

PCR

polymerase chain reaction

PEG

polyethylene glycol

pI

isoelectric point

PMSF

phenylmethylsulfonyl fluoride

PVDF

polyvinylidene difluoride

Q-TOF

quadrupole time-of-flightRNAribonucleic acidRIA

radioimmunoassay

rpm

revolutions per minute

RT-PCR

reverse transcriptase PCRSD

standard deviation

SDS

sodium dodecyl sulfate

S/Nsignal-to-noise ratio

SSCP

single-strand conformation polymorphism

ssDNA

single-stranded DNA%T

total gel concentration (acrylamide plus cross-linking agent, g/100 mL)TBStris-buffered saline

TEMED

N,N,N',N'-tetramethylethylenediamine

TOF

Time-of-flightTristris(hydroxymethyl)aminomethane8