ANNUAL PUBLICATIONS
2016

Graduate School of
Medical and Dental Sciences
Tokyo Medical and Dental University

CONTENTS
Graduate School of Medical and Dental Sciences
Medical and Dental Sciences
Oral Health Sciences

Oral Pathology………………………………………………………………………………………………… 1
Bacterial Pathogenesis, Infection and Host Response… ………………………………………………… 3
Molecular Immunology… …………………………………………………………………………………… 5
Advanced Biomaterials… …………………………………………………………………………………… 7
Diagnostic Oral Pathology………………………………………………………………………………… 10
Oral Radiation Oncology…………………………………………………………………………………… 12
Oral and Maxillofacial Surgery…………………………………………………………………………… 14
Oral and Maxillofacial Radiology… ……………………………………………………………………… 17
Anesthesiology and Clinical Physiology… ……………………………………………………………… 19
Orofacial Pain Management… …………………………………………………………………………… 22
Pediatric Dentistry………………………………………………………………………………………… 24
Orthodontic Science… …………………………………………………………………………………… 27
Cariology and Operative Dentistry……………………………………………………………………… 34
Fixed Prosthodontics… …………………………………………………………………………………… 42
Pulp Biology and Endodontics… ………………………………………………………………………… 46
Removable Partial Prosthodontics… …………………………………………………………………… 53
Oral Implantology and Regenerative Dental Medicine… …………………………………………… 59

Maxillofacial and Neck Reconstruction

Plastic and Reconstructive Surgery………………………………………………………………………
Head and Neck Surgery……………………………………………………………………………………
Radiation Therapeutics and Oncology……………………………………………………………………
Maxillofacial Anatomy… …………………………………………………………………………………
Cognitive Neurobiology… …………………………………………………………………………………
Molecular Craniofacial Embryology………………………………………………………………………
Cellular Physiological Chemistry…………………………………………………………………………
Biodesign… …………………………………………………………………………………………………
Maxillofacial Surgery… ……………………………………………………………………………………
Maxillofacial Orthognathics… ……………………………………………………………………………
Maxillofacial Prosthetics……………………………………………………………………………………

62
65
68
70
72
75
77
79
80
84
89

Bio-Matrix

Cell Biology… ……………………………………………………………………………………………… 94
Medical Biochemistry……………………………………………………………………………………… 96
Joint Surgery and Sports Medicine……………………………………………………………………… 98
Biostructural Science… …………………………………………………………………………………… 104
Pharmacology… …………………………………………………………………………………………… 105
Connective Tissue Regeneration… ……………………………………………………………………… 107
Biochemistry………………………………………………………………………………………………… 109
Cell Signaling… …………………………………………………………………………………………… 113
Periodontology……………………………………………………………………………………………… 115

Public Health

Global Health Promotion…………………………………………………………………………………… 124
Environmental Parasitology… …………………………………………………………………………… 129
Forensic Medicine… ……………………………………………………………………………………… 132
Health Care Management and Planning………………………………………………………………… 134
Molecular Epidemiology…………………………………………………………………………………… 136
Research Development… ………………………………………………………………………………… 138
Health Policy and Informatics… ………………………………………………………………………… 140
Life Sciences and Bioethics… …………………………………………………………………………… 145
Forensic Dentistry… ……………………………………………………………………………………… 147
Health Care Economics… ………………………………………………………………………………… 150
Dental Education Development…………………………………………………………………………… 152
Oral Health Promotion… ………………………………………………………………………………… 154
Sports Medicine and Dentistry…………………………………………………………………………… 158
Educational Media Development………………………………………………………………………… 161iInsured Medical Care Management……………………………………………………………………… 163
Global Health Entrepreneurship… ……………………………………………………………………… 167

Gerontology and Gerodontology

Geriatrics and Vascular Medicine………………………………………………………………………… 170
Rehabilitation Medicine… ………………………………………………………………………………… 172
Gerodontology and Oral Rehabilitation… ……………………………………………………………… 174

Comprehensive Patient Care

Laboratory Medicine… …………………………………………………………………………………… 180
Intensive Care Medicine…………………………………………………………………………………… 183
Liaison Psychiatry and Palliative Medicine… ………………………………………………………… 186
Pharmacokinetics and Pharmacodynamics……………………………………………………………… 189
Medical Education Research and Development………………………………………………………… 190
Acute Critical Care and Disaster Medicine… ………………………………………………………… 193
Clinical Oncology…………………………………………………………………………………………… 195
Dentistry for Persons with Disabilities… ……………………………………………………………… 197
General Dentistry… ……………………………………………………………………………………… 199
Psychosomatic Dentistry… ……………………………………………………………………………… 203
Behavioral Dentistry… …………………………………………………………………………………… 206
Professional Development in Health Sciences… ……………………………………………………… 208

Cognitive and Behavioral Medicine

Neuroanatomy and Cellular Neurobiology……………………………………………………………… 211
Systems Neurophysiology………………………………………………………………………………… 213
Pharmacology and Neurobiology………………………………………………………………………… 215
Molecular Neuroscience…………………………………………………………………………………… 217
Neuropathology… ………………………………………………………………………………………… 220
Ophthalmology and Visual Science… …………………………………………………………………… 224
Otorhinolaryngology… …………………………………………………………………………………… 229
Neurology and Neurological Science… ………………………………………………………………… 231
Psychiatry and Behavioral Sciences……………………………………………………………………… 238
Neurosurgery… …………………………………………………………………………………………… 242
Endovascular Surgery……………………………………………………………………………………… 246
NCNP Brain Physiology and Pathology… ……………………………………………………………… 248

Bio-Environmental Response

Immune Regulation………………………………………………………………………………………… 255
Molecular Virology………………………………………………………………………………………… 258
Immunotherapeutics… …………………………………………………………………………………… 261
Cellular and Environmental Biology……………………………………………………………………… 263
Biodefense Research… …………………………………………………………………………………… 264
Pathological Cell Biology… ……………………………………………………………………………… 267
Pediatrics and Developmental Biology… ……………………………………………………………… 270
Rheumatology… …………………………………………………………………………………………… 285
Immunology → Refer to Immunology (page461〜462)
Dermatology………………………………………………………………………………………………… 290
NCCHD Child Health and Development………………………………………………………………… 295

Systemic Organ Regulation

Human Pathology………………………………………………………………………………………… 300
Physiology and Cell Biology… …………………………………………………………………………… 303
Molecular Cellular Cardiology… ………………………………………………………………………… 306
Stem Cell Regulation… …………………………………………………………………………………… 310
Molecular Pharmacology… ……………………………………………………………………………… 313
Stem Cell Biology…………………………………………………………………………………………… 315
Respiratory Medicine……………………………………………………………………………………… 317
Gastroenterology and Hepatology… …………………………………………………………………… 319
Specialized Surgeries… …………………………………………………………………………………… 330
Cardiovascular Medicine… ……………………………………………………………………………… 333
Anesthesiology……………………………………………………………………………………………… 336
Cardiovascular Surgery…………………………………………………………………………………… 338
Nephrology… ……………………………………………………………………………………………… 342
Comprehensive Reproductive Medicine………………………………………………………………… 347

iiUrology……………………………………………………………………………………………………… 352
Gastrointestinal Surgery…………………………………………………………………………………… 359
Molecular Cell Biology → Refer to Molecular Cell Biology (page458)
Chronobiology → Refer to Developmental and Regenerative Biology (page459〜460)
Thoracic Surgery…………………………………………………………………………………………… 363
Igakuken Disease-oriented Molecular Biology… ……………………………………………………… 365

Advanced Therapeutic Sciences

Clinical Anatomy…………………………………………………………………………………………… 370
Systems BioMedicine……………………………………………………………………………………… 373
Comprehensive Pathology………………………………………………………………………………… 377
Molecular Oncology………………………………………………………………………………………… 379
Surgical Pathology… ……………………………………………………………………………………… 383
Experimental Animal Model for Human Disease……………………………………………………… 387
Signal Gene Regulation… ………………………………………………………………………………… 388
Biomedical Devices and Instrumentation→ Refer to Biomedical Devices and Instrumentation (page438〜440)
Material Biofunctions……………………………………………………………………………………… 390
Genetic Regulation… ……………………………………………………………………………………… 393
Applied Gene Medicine… ………………………………………………………………………………… 395
Molecular Cytogenetics… ………………………………………………………………………………… 397
Hematology… ……………………………………………………………………………………………… 399
Molecular Endocrinology and Metabolism……………………………………………………………… 403
Hepatobiliary and Pancreatic Surgery…………………………………………………………………… 406
Orthopaedic and Spinal Surgery… ……………………………………………………………………… 411
Medical Instrument → Refer to Biomedical Information (page441)
Biointerface Engineering → Refer to Bioelectronics (page442〜446)
Diagnostic Radiology and Nuclear Medicine… ………………………………………………………… 418
Human Genetics and Disease Diversity… ……………………………………………………………… 425
Applied Regenerative Medicine… ……………………………………………………………………… 428
JFCR Cancer Biology……………………………………………………………………………………… 431
Minimally Invasive Medical Treatment………………………………………………………………… 436

Life Science and Technology
Life Science and Technology

Biomedical Devices and Instrumentation… …………………………………………………………… 438
Biomedical Information… ………………………………………………………………………………… 441
Bioelectronics… …………………………………………………………………………………………… 442
Material-Based Medical Engineering… ………………………………………………………………… 447
Organic and Medicinal Chemistry… …………………………………………………………………… 450
Chemical Bioscience… …………………………………………………………………………………… 454
Biomechanics… …………………………………………………………………………………………… 456
Molecular Cell Biology… ………………………………………………………………………………… 458
Developmental and Regenerative Biology… …………………………………………………………… 459
Immunology………………………………………………………………………………………………… 461
Epigenetics… ……………………………………………………………………………………………… 463
Medical Science Mathematics… ………………………………………………………………………… 465
Structural Biology… ……………………………………………………………………………………… 471
Neuroscience………………………………………………………………………………………………… 473
Molecular Genetics → Refer to Applied Gene Medicine (page395〜396)
Epigenetic Epidemiology… ……………………………………………………………………………… 476
RIKEN Molecular and Chemical Somatology…………………………………………………………… 478
Metallic Biomaterials… …………………………………………………………………………………… 482
Inorganic Biomaterials… ………………………………………………………………………………… 486
Organic Materials… ……………………………………………………………………………………… 489
Medicinal Chemistry… …………………………………………………………………………………… 491
NCC Cancer Science… …………………………………………………………………………………… 495
Cellular and Molecular Medicine………………………………………………………………………… 499

Medical and Dental Science and Technology

Lifetime Oral Health Care Sciences……………………………………………………………………… 502
Oral Care for Systemic Health Support… ……………………………………………………………… 504
Preventive Oral Health Care Sciences… ……………………………………………………………… 506

iii

Oral Health Science for Community Welfare…………………………………………………………… 508
Oral Health Care Education… …………………………………………………………………………… 510
Basic Sciences of Oral Health Care……………………………………………………………………… 512
Basic Oral Health Engineering…………………………………………………………………………… 514
Oral Biomaterials Development Engineering…………………………………………………………… 517
Oral Prosthetic Engineering……………………………………………………………………………… 519

Endowed Departments

Department of Nanomedicine… ………………………………………………………………………… 521
Department of Liver Disease Control…………………………………………………………………… 523
Department of Cartilage Regeneration → Refer to Joint Surgery and Sports Medicine (page98〜103)
Department of Advanced Therapeutics for GI Diseases……………………………………………… 528
Department of Orthopaedic Research and Development→ Refer to Orthopaedic and Spinal Surgery (page411〜417)
Department of Sleep Modulatory Medicine… ………………………………………………………… 532
Department of Joint Reconstruction → Refer to Joint Surgery and Sports Medicine (page98〜103)
Department of Women’s Health… ……………………………………………………………………… 534
Department of Advanced Surgical Technology Research and Development……………………… 536
Department of Arteriosclerosis and Vascular Biology………………………………………………… 538

Joint Research Departments

Department of Translational Oncology… ……………………………………………………………… 540

Medical Hospital
Central Clinical Facilities

Clinical Laboratory………………………………………………………………………………………… 542
Radiology Center → Refer to Radiation Therapeutics and Oncology (page68〜69)
Transfusion Medicine……………………………………………………………………………………… 546
General Medicine・Professional Development Center→ Refer to Medical Education Research and Development (page190〜192)
Hyperbaric Medical Center… …………………………………………………………………………… 548
Center for Cell Therapy…………………………………………………………………………………… 551

Dental Hospital
Hospital Departments

Cleanroom…………………………………………………………………………………………………… 558
Center for Development of Devices and Drugs in Dentistry………………………………………… 559

Sports Science Organization

Clinical Center for Sports Medicine and Sports Dentistry…………………………………………… 562

iv

Oral
Oral Health
Health Sciences
Sciences

Oral Pathology

Professor
Tohru Ikeda
Junior Associate Professor
Kei Sakamoto
Assistant Professor
Kou Kayamori
Technical Staﬀ
Miwako Hamagaki
Graduate Students
Akane Yukimori
Sawangarun Wanlada
Yae Ohata
Yuko Ymagata (Oral and Maxillofacial Surgery)
Akane Wada
Shoko Ishida
Maiko Tsuchiya(1)

Research

1) Pathology and biology associated with bone2) Pathological and biological studies on oral cancers, odontogenic tumors and oral premalignant lesions
3) Pathological and biological studies on microenvironment associated with invasion and metastasis of cancers4) Clinicopathological and diagnostic histopathological studies on oral and maxillofacial lesions(2)

Education

Lectures and microscope practice in the module Pathology to 3rd grade students. The Pathology module
comprises two sections; General pathology and Oral pathology. Main objective of General pathology is toprovide students knowledge on various diseases, which is essential to work in dental, medical and biologicalﬁelds. Oral pathology provides detailed knowledge on oral diseases, which is indispensable for a dentist.
(3)

Clinical Services & Other Works

Our staﬀs and graduate students participate in diagnostic pathology practice in the Dental Hospital, where
nearly 3,000 specimens are annually submitted to laboratory investigation. Our staﬀs and graduate students
also participate in autopsy in the Medical Hospital in cooperation with the staﬀs and graduate students at theFaculty of Medicine.

―1―Oral Health
Health Sciences
Sciences
Oral(4)

Publications[Original Articles]
1. Kou Kayamori, Ken-Ichi Katsube, Kei Sakamoto, Yoshio Ohyama, Hideaki Hirai, Akane Yukimori, Yae
Ohata, Takumi Akashi, Masao Saitoh, Kiyoshi Harada, Hiroyuki Harada, Akira Yamaguchi. NOTCH3 Is
Induced in Cancer-Associated Fibroblasts and Promotes Angiogenesis in Oral Squamous Cell Carcinoma.
PLoS ONE. 2016; 11(4); e0154112
2. Masita Mandasari, Wanlada Sawangarun, Ken-Ichi Katsube, Kou Kayamori, Akira Yamaguchi, Kei
Sakamoto. A facile one-step strategy for the generation of conditional knockout mice to explore therole of Notch1 in oroesophageal tumorigenesis. Biochem. Biophys. Res. Commun.. 2016.01; 469(3);761-767―2―

Oral
Oral Health
Health Sciences
Sciences

Bacterial Pathogenesis

Professor SUZUKI Toshihiko
Assistant Professor
SUZUKI Shiho(1)

ResearchResearch Subjects
1) Molecular mechanisms of infection by pathogenic bacteria2) Mechanisms of activation and regulation of inﬂammasomes via Nod-like receptors and caspase activation
3) Study of virulent genes based on comparative genomics
4) Relationship between persistent bacterial infection and chronic inﬂammatory diseases such as adipose ordiabetes(2)

Lectures & Courses

Purpose of Education
The aim of our laboratory in the graduate course is to understand molecular mechanism of pathogen infection
and host immune responses. Students also learn planning of research, experiments and methods for evaluating.
(3)

Publications[Original Articles]
1. Ayumi Saeki, Toshihiko Suzuki, Akira Hasebe, Ryousuke Kamezaki, Mari Fujita, Futoshi Nakazawa, KenIchiro Shibata. Activation of nucleotide-binding domain-like receptor containing protein 3 inﬂammasome
in dendritic cells and macrophages by Streptococcus sanguinis. Cell. Microbiol.. 2016.09; Epub;
2. Yohei Yamaguchi, Tomoko Kurita-Ochiai, Ryoki Kobayashi, Toshihiko Suzuki, Tomohiro Ando. Regulation of the NLRP3 inﬂammasome in Porphyromonas gingivalis-accelerated periodontal disease. Inﬂamm.
Res.. 2016.09; 66(1); 59-65[Books etc]
1. Toshihiko Suzuki etc.. Antibiotics and Chemotherapy. Iyaku Journal-sha, 2016.02
2. Shiho Suzuki, Toshihiko Suzuki. Antibiotics and Chemotherapy . Iyaku Journal-sha , 2016.09[Conference Activities & Talks]
1. Shiho Suzuki, Toshihiko Suzuki, Hitomi Mimuro, Chihiro Sasakawa. GLMN is novel negative regulatorof inﬂammasome in response to bacterial infection. The 89th Annual Meeting of Japanese Society for
Bacteriology 2016.03.23 Osaka―3―

Oral Health
Health Sciences
Sciences
Oral

2. Ayumi Saeki, Akira Hasebe, Toshihiko Suzuki, Ken-ichiro Shibata. IL-1alpha-inducing activity of Streptococcus sanguinis in murine dendritic cells and macrophages. The 89th Annual Meeting of JapaneseSociety for Bacteriology 2016.03.25 Osaka
3. Ryosuke Kamezaki, Ayumi Saeki, Akira Hasebe, Yoshimasa Kitagawa, Toshihiko Suzuki, Ken-ichiro Shibata. Activation of inﬂammasome by Aggregatibacter actinomycetemcomitans. The 58th Annual Meetingof Japanese Association for Oral Biology 2016.08.24 Sapporo
4. Ayumi Saeki, Akira Hasebe, Ryosuke Kamezaki, Toshihiko Suzuki, Ken-ichiro Shibata. Involvement ofinﬂammasome in IL-1alpha-inducing activity of Streptococcus sanguinis. The 58th Annual Meeting ofJapanese Association for Oral Biology 2016.08.24 Sapporo
5. Shiho Suzuki, Toshihiko Suzuki, Hitomi Mimuro, Chihiro Sasakawa. GLMN is functionally involved ininﬂammasome activation. The 45th Annual Meeting of The Japanese Society for Immunology 2016.12.07
Okinawa―4―

Oral
Oral Health
Health Sciences
Sciences

Molecular Immunology

Professor
Associate Professor
Assistant Professor
Adjunct instructor

Miyuki Azuma
Shigenori Nagai
Tatsukuni Ohno
Hiroshi Kiyono
Takeshi Azuma
Graduate Students (Doctor)
Siwen Kang(∼Sept.)
Yuta Kondo(∼Mar.)
Hirunwidchayarat Worawalun
Nadya Niken Adiba
Naoto Nishii
Xia Yulong
Katou Hiroshi(Apr.∼)Furusawa Emi(Apr.∼)Yang Yue(Apr.∼)Tachinami Hidetake(Apr.∼)Research Student
Jin Xin(1)

ResearchResearch Subjects
1) Mechanisms of immune responses in oral diseases
2) Studies on lymphocyte functional molecules3) Immunotherapy by molecular targetting(2)

Lectures & Courses

Purpose of Education
Main objective of Molecular Immunology in the graduate course is to understand and study how the immune
system works for biological defense. Students also learn immunopathology and immunophysiology of systemic
and organ-speciﬁc immune diseases and how the immune diseases control and regulate.

(3)

Publications[Original Articles]
1. Kondo Y, Ohno T, Nishii N, Harada K, Yagita H, Azuma M. Diﬀerential contribution of three immune
checkpoint (VISTA, CTLA-4, PD-1) pathways to antitumor responses against squamous cell carcinoma.
Oral Oncology. 2016; 57; 54-60

―5―

Oral
HealthSciences
Oral
HealthSciences

2. Kurebayashi Y, Baba Y, Minowa A, Nadya NA, Azuma M, Yoshimura A, Koyasu S, Nagai S. TGFβ-induced phosphorylation of Akt and Foxo transcription factors negatively regulates the diﬀerentiationof induced regulatory T cells. Biochem Biophys Res Commun. 2016; 480; 114-119
3. Shiono Y, Ishii K, Nagai S, Kakinuma H, Sasaki A, Tsuji T, Okada Y, Koyasu S, Nakamura M, Toyama
Y, Aizawa M, Matsumoto M. Delayed Propionibacterium acnes surgical site infections occur only in thepresence of an implant. Sci Rep. 2016; 6; 32758
4. Yamazumi Y, Sasaki O, Imamura M, Oda T, Ohno Y, Shiozaki-Sato Y, Nagai S, Suyama S, Kamoshida Y,
Funato K, Yasui T, Kikutani H, Yamamoto K, Koyasu S, Akiyama T. The RNA-binding protein Mex-3B
is required for IL-33 induction in the development of allergic airway inﬂammation. Cell Rep. 2016; 16;2456-2471
5. Funao H, Nagai S, Sasaki A, Hoshikawa T, Tsuji T, Okada Y, Koyasu S, Toyama Y, Nakamura M, Aizawa
M, Matsumoto M, Ishii K. A novel hydroxyapatite ﬁlm coated with ionic silver via inositol hexaphosphatechelation prevents implant-associated infection. Sci Rep. 2016; 6; 23238
6. Vo M, Holz LE, Wong YC, English K, Benseler V, McGuﬀog C, Azuma M, McGaughan GW, Bowen DG,
Bertolino P. Eﬀector T cell function rather than survival determines extent and duration of hepatitis inmice. J Hapatol. 2016; 64; 1327-1338
7. Kang S, Zhang C, Ohno T, Azuma M. Unique B7-H1 expression on masticatory mucosae in the oral cavity
and trans-coinhibition by B7-H1-expressing keratinocytes regulating CD4+ T-cell-mediated mucosal tissue
inﬂammation. Mucosal Immunol; in press. 2016;[Conference Activities & Talks]
1. Azuma M. Immune regulation in the oral cavity. Tokyo medical and Dental University-Taipei Medical
University Joint Symposium 2016.11.12 Taiwan

―6―

Oral
Oral Health
Health Sciences
Sciences

Advanced Biomaterials

Professor UO Motohiro
Associate Professor HONGO Toshio
Assistant Professor NAKAMURA Hideo
Assistant Professor WADA Takahiro
Graduate Student CHAIAMORNSUP Patcharanun(1)

Research

1. Analysis of Dental and biomedical materials and biological tissue using the synchrotron radiation.
Research is aimed to apply the new analysis method using synchrotron radiation for the estimation of various
properties of the dental and biomedical materials.
2. Development of the functional dental and biomedical materials using glass and ceramics.
Research is aimed to develop and evaluate the new glass and ceramics based materials as the dental andbiomedical materials, e.g. composite resins, glass ionomer cements, dental porcelains and zirconia ceramics.
(2)

Education

1. Lecture of unit Biomaterials and Dental Materials
A series of lectures on the science on biomaterials , properties of dental and biomedical materials ,application of dental materials will be taught through the lecture and practice.
2. Lecture of unit Advanced Biomaterials (graduate school)
Evaluation methods of various dental and biomedical materials will be taught.

(3)

Publications[Original Articles]
1. Motohiro Uo. Distribution analyses of trace metallic elements in oral mucosal tissues using high-energy
SR-XRF SPring-8 Research Frontiers 2015. 2016;
2. Takahiro Wada, Naoyoshi Murata, Hiromitsu Uehara, Takuya Suzuki, Hiroaki Nitani, Yasuhiro Niwa,
Motohiro Uo, Kiyotaka Asakura. Degradation mechanism of a high-performance real micro gas sensor, as
determined by spatially resolved XAFS Physical Chemistry Chemical Physics. 2016.02; 18; 7374-7380
3. Satoru Takakusagi, Akitoshi Kunimoto, Natee Sirisit, Hiromitsu Uehara, Tadashi Ohba, Yohei Uemura,
Takahiro Wada, Hiroko Ariga, Wang-Jae Chun, Yasuhiro Iwasawa, Kiyotaka Asakura. A New Indicatorfor Single Metal Dispersion on a TiO2 (110) Surface Premodiﬁed with a Mercapto Compound Journal of
Physical chemistry C. 2016.03;
4. Kitano R., Ishii M., Uo M., Morita K. Thermodynamic Properties of the CaO–AlO1.5–CeO1.5 System
ISIJ International. 2016.10;

―7―

Oral
HealthSciences
Oral
HealthSciences

5. Takahiro Wada, Hiroshi Churei, Toshiaki Ueno, Motohiro Uo. High shock absorbing faceguard usingﬁber-reinforced plastic (FRP) and elastomer The Journal of the Japanese Society for Dental Materials
and Devices. 2016.11; 35(6); 325-328
6. WeiJen Lai, Yoshiyuki Midorikawa, Zuisei Kanno, Hiroshi Takemura, Kazuhiro Suga, Kohei Soga, Takashi
Ono, Motohiro Uo. Development and modiﬁcation of a device for three-dimensional measurement oforthodontic force system: The V-bend system re-visited. Dent Mater J. 2016.12; 35(6); 908-917[Misc]
1. Takahiro WADA, Naoyoshi MURATA, Hiromitsu UEHARA, Takuya SUZUKI, Hiroaki NITANI, Yasuhiro
NIWA, Motohiro UO, Kiyotaka ASAKURA. XAFS Analysis of Pt-SnO2 and Pd/Al2 O3 catalysts in theworld’s ﬁrst battery driven gas sensor PF News. 2016.02; 33(4); 13-19
2. Motohiro Uo. Distribution analyses of trace metallic elements in oral mucosal tissues using high-energy
SR-XRF SPring-8 Research Frontiers 2015. 2016.08; 19-20[Conference Activities & Talks]
1. Rui Guan, Tomohiro Takagaki, Toru Nikaido, Takahiro Wada, Motohiro Uo, Junji Tagami. Adsorption
behavior of phosphoric functional monomers to 3Y-TZP surface.. 45th Annual meeting of American
Association for Dental Research 2016.03.17
2. Takahiro Wada, Hiroshi Churei, Shintaro Fukasawa, Takahiro Shirako, Toshiaki Ueno, Motohiro Uo.
Shock-absorbing property of face guard by incorporating a glass-ﬁber reinforced plastic and space. 2016.06.12
3. M. YAMAMOTO, K. MOTOMURA, H. NAKAMURA, T. YOSHIOKA, F. YOSHIKAWA, S. TANAKA.
Optimum conditions for resistance-heat-bending of NiTi arch wire. 94rd General Session & Exhibition of
the IADR, 3rd Meeting of the Asia Paciﬁc Region, 35th Annual Meeting of the IADR Korean Division
2016.06.23 Seoul
4. F.YOSHIKAWA, K.MOTOMURA, H.NAKAMURA, T.YOSHIOKA, M.YAMAMOTO, S.TANAKA. Improving adhesion of zirconia by surface modiﬁcation. 94rd General Session & Exhibition of the IADR,
3rd Meeting of the Asia Paciﬁc Region, 35th Annual Meeting of the IADR Korean Division 2016.06.24
Seoul
5. Midorikawa Y, Takemura H, Mizoguchi H, Soga K, Kamimura M, Suga K, Lai WJ, Kanno Z, Uo M.
Six-axis orthodontic force and moment sensing system for dentist technique training. IEEE 38th Annual
International Conference 2016.08.16
6. Qiuyi Yuan, Satoru Takakusagi, Yohei Uemura, Takahiro Wada, Kiyotaka Asakura. A XAFS Investigationof Pt Monolayer on Au(111) Obtained from Displacement Reaction. PRiME 2016/230th ECS Meething
2016.10.03
7. Uo M, Wada T, Maekawa M, Kanno Z, Ono T, Hanawa T. Development of super engineering plasticV(PEEK) made orthodontic wires. The 1st International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development (iLIM-1) 2016.10.17 Osaka, Japan
8. Takahiro Wada, Hiroshi Churei, Haruka Takayanagi, Mako Yokose, Shitaro Fukasawa, Takahiro Shirako,
Toshiaki Ueno, Naohiko Iwasaki, Hidekazu Takahashi, Motohiro Uo. Development of a faceguard using a
glass-ﬁber reinforced thermoplastic (GFRTP) and shock escape space. Visual-JW 2016 2016.10.17 Suita,
Osaka, Japan
9. Donghoon Kang, Takahiro Wada, Motohiro Uo, Takashi Okiji. Evaluation of mechanical properties of anexperimental synthetic tricalcium silicate cement. 2016.10.22 Seoul, Korea
10. Takahiro Wada, Hiroshi Churei, Shintaro Fukasawa, Takahiro Shirako, Toshio Hongo, Toshiaki Ueno,
Motohiro Uo. Analysis of shock absorption ability of a faceguard by incorporating a glass-ﬁber reinforced
thermoplastic and space. International Dental Materials Congress 2016 2016.11.05 Bali, Indonesia
11. Tanabe G, Churei H, Wada T, Uo M, Takahashi H, Ueno T. Eﬀect of the use of commercial cleaning
liquid in thermoforming process of mouthguard materials. International Dental Materials Congress 20162016.11.05 Bali, Indonesia

―8―

Oral
Oral Health
Health Sciences
Sciences

12. Toshio Hongo, Takahiro Wada, Motohiro Uo. Development of a quantitative method of residual substancesin the autopolymerizing acrylic resin. International Dental Materials Congress 2016 2016.11.05 Bali,
Indonesia―9―Oral Health Sciences

Oral Health Sciences

Diagnostic Oral Pathology

Associate Professor: Toshiyuki IZUMO
Visiting Lecturer:
Yasuo YAGISHITA, Yasumasa MORI
Hospital Stuﬀ:
Rei TOHYAMA,
Yuuichi YAMADA, Kiyoko NAGUMO,
Kana NANBA, Akiko ASANO,
Mayuko MINAMI, Yukiko Kuroki,
Kou YANAI, Yuuta TAKAHASHI(1)

OutlineDiagnostic oral pathology is a branch of pathology which studies human pathology, and aims at practice anddevelopment of the oral science as clinical medicine. The main object is to bring up graduate students andpost-doctoral residents for pathology specialist to the great oral pathologists through the lecture of surgicalpathology and pathology diagnosis and research instruction of oral and general diseases for the time being.
(2)

ResearchResearch Subjects
1) Surgical pathology of oral cancer.
2) New diagnostic approach and reconstruction of oral diseases.

(3)

Clinical Services & Other Works

Diagnostic oral pathology is playing three roles, pathological diagnosis (3,400 cases in a year), clinical laboratory (215,000 tests in a year) which consist of hematological, biochemical, bacteriological, physiological andpathological parts, and blood transfusion (100 cases in a year) in the dental hospital.
(4)

Publications[Original Articles]
1. Nguyen CT，Okamura T，Morita K，Yamaguchi S，Harada H，Miki Y，Izumo T，Kayamori K，Yamaguchi
A，Sakamoto K. LAMC2 is a predictive marker for the malignant progression of leukoplakia Journal of
Oral Pathology and Medicine. 2016;
2. Okamura T, Izumo T, Yagishita H, Mori T, Sakamoto K, Harada K.. Disordered arrangements of basal
cells as a prognostic factor for oral epithelial dysplasia: a morphometric study of 96 cases. Oral Surg Oral
Med Oral Pathol Oral Radiol. . 2016; 122(3); 355-361
3. Morita T, Uzawa N, Mogushi K, Sumino J, Michikawa C, Takahashi KI, Myo K, Izumo T, Harada K..
Characterizing Genetic Transitions of Copy Number Alterations and Allelic Imbalances in Oral Tongue
Carcinoma Metastasis. Genes Chromosomes Cancer. 2016; 55(12); 975-986― 10 ―

Oral Health Sciences

Oral Health Sciences

4. Morita T, Yamashiro M, Kayamori K, Mizutani M, Nakakuki K, Michi Y, Uzawa N, Izumo T, Harada K.
Primary intraosseous squamous cell carcinoma derived from a maxillary cyst: A case report and literature
review. Molecular and Clinical Oncology. 2016; 4; 553-558
5. Wake S, Yoshitake H, Kayamori K, Izumo T, Harada K. Expression of CD90 decreases with progressionof synovial chondromatosis in the temporomandibular joint. Cranio. 2016; 34(4); 250-256― 11 ―

Oral Health Sciences

Oral Health Sciences

Oral Radiation Oncology

Professor
Assistant Professor
Clinical Fellow
Graduate Students

Special Research Student(1)

Masahiko MIURA
Atsushi KAIDA
Satoshi SATO
Tatuaki GOTOU
Kouhei OKUYAMA
Hiroyuki ONOZATO
Nisha GOWRI MANILA
Sirimanas JIARANUCHART
Hisao HONMA
Taito ASAHINA

OutlineMain objective of this branch is to provide opportunities to study radiation oncology for oral cancer andtranslational research for radiosensitization of oral cancer.
(2)

Research

1) Visualization of tumor radioresponse by moleculr imaging
2) Mechanism of DNA damage response3) Radioresistant signal transduction pathways
4) Radiotherapy for oral cancer(3)

Education

Oral Radiation Oncology is a branch of radiation oncology dealing with basic radiobiology, translational research,and radiotherapy for oral cancer. Main objective of this branch in the graduate course is to provide opportunities
to study biological strategies for radiosensitization, development of radiosensitizers, molecular mechanism oftumor radioresistance, the state of the art technology of radiotherapy, and basis of individualized radiotherapy
depending on each student s research projects.
(4)

Lectures & Courses

The educational policy is to cultivate researchers to be able to extract problems and to work out solutions to
them.
(5)

Clinical Services & Other Works

Oral Radiation Oncology clinic provides radiotherapeutic treatment for head and neck cancer patients, especiallybrachytherapy for oral cancer, in cooperation with Diagnostic and Therapeutic Radiology clinic in the Medical
Hospital.

― 12 ―

Oral Health Sciences

Oral Health Sciences(6)

Clinical Performances

We are performing brachytherapy for oral cancer, which is now the only treatment modality without surgicalexcision, as a center institution in Japan.
(7)

Publications[Original Articles]
1. Taito Asahina, Atsushi Kaida, Tatsuaki Goto, Ryo-Ichi Yoshimura, Keisuke Sasai, Masahiko Miura.
Temporo-spatial cell-cycle kinetics in HeLa cells irradiated by Ir-192 high dose-rate remote afterloading
system (HDR-RALS). Radiat Oncol. 2016.07; 11; 99
2. Nisha Gowri Manila, Atsushi Kaida, Masahiko Miura. Kinetic analysis of radiation-induced cell-cycle
alterations in HeLa cells expressing ﬂuorescent ubiquitination-based cell cycle indicator (Fucci). Radiat
Environ Med. 2016.08; 5(2); 16-21
3. Atsushi Kaida, Hiroshi Watanabe, Kazuma Toda, Keiko Yuasa-Nakagawa, Ryoichi Yoshimura, Masahiko
Miura. Eﬀects of dose rate on early and late complications in low dose rate brachytherapy for mobile
tongue carcinoma using Ir-192 sources. Oral Radiol. 2016.12; published online;[Conference Activities & Talks]
1. Watanabe H, Ozaki Y, Miura M, Yoshimura R, Sumi Y, Kurabayashi T. Estimation of whole bodyradiation exposure induced by oral cancer brachytherapy using Monte Carlo simulation. The 11th AsianCongress of Oral and Maxillofacial Radiology 2016.11.12 Chiang Mai, Thailand
2. Yusuke Onozato, Atsushi Kaida, Hiroyuki Harada, Masahiko Miura. Radiosenstivity of proliferatingor quiescent human tongue carcinoma cells grown in multicellular spheroids. The 61th congress of theJapanese Society of Oral and Maxillofacial Surgeons 2016.11.25 Chiba city
3. Masahiko Miura. Cell cycle kinetics and radiosensitivity. The 29th Annual Meeting of JASTRO 2016.11.27
Kyoto city
4. Nisha Gowri Manila, Atsushi Kaida, Masahiko Miura. IGF-IR, but not EGFR, regulates DNA damage
response (DDR) in HeLa cells following irradiation. ESMO Asia 2016 Congress 2016.12.17 Singapore― 13 ―

Oral Health Sciences

Oral Health Sciences

Oral and Maxillofacial Surgery

Professor Hiroyuki HARADA
Junior Associate Professor Yuji KABASAWA, Eriko MARUKAWA
Assistant Professor Hiroaki SHIMAMOTO, Fumihiko TSUSHIMA, Hirofumi TOMIOKA, Hideaki HIRAI,
Kae TANAKA
Graduate Student
Yu OIKAWA, Yusuke ONOZATO, Toru TAKEMOTO, Yuko YAMAGATA,
Naoto NISHII，JIARANUCHART Sirimanas, Shuhei FUKUDA,
Ruri KOMIYA, Yoshihisa KASHIMA, Hitomi NOJIMA,
Misaki YOKOKAWA, Naoya KINOSHITA, Yuuki TAKAGAWA,
Aoi KANEKO(1)

OutlinePurpose of Education
The program is designed for acquiring the broad knowledge and basic skills of oral and maxillofacial surgery,
mainly concerning the diagnostic procedure, treatment technique and the perioperative patient care. Also
throughout the professional education, we promote the system in which each graduate student can select his or
her special ﬁeld in the full scope of oral and maxillofacial surgery in the future.
Research Subjects
1) Development of multidisciplinary treatment of oral cancer.
2) Clinical study on sentinel node navigation surgery for oral cancer.
3) Study on molecular markers for lymph node metastasis of oral cancer.
4) Clinical study on skeletal and dental changes after distraction osteogenesis in patients with cleft lip andpalate.
5) Clinical study on maxillomandibular skeletal and dental changes after orthognatic surgery.
6) Study on neurosensory disturbances using the heat ﬂux technique.
7) Clinical study on pre-surgical nasoalveolar molding in patients with cleft lip and palate.
8) Clinical study on alveolar bone grafting with platelet rich plasma.
9) Multidisciplinary treatment of temporomandibular disorders.
10) Clinical and experimental studies on bone regeneration using β-TCP and/or platelet rich plasma.
11) Development of multidisciplinary treatment of oral mucosal diseases.
Clinical Services
The Oral and Maxillofacial Surgery Clinic examines yearly more than 6,200 new patients with various diseases
arising in oral and maxillofacial regions. The clinic has diplomat of the Japanese Society of Oral and Maxillofacial Surgeons and accepts many referrals from dentists and medical doctors. We provide a full range of services
including extractions, removal of wisdom teeth and management of facial trauma, jawbone defect, facial deformity, temporomandibular joint disease, cleft lip and palate, oral mucosal disease, and benign and malignant
tumors. The special outpatient clinics are organized by the specialists to oﬀer the best service, especially forpatients with malignant tumor, temporomandibular joint disease, cleft lip and palate, facial deformity and oral
mucosal disease which need high degree of specialty and long term follow up. We also prepare some groups for
inpatients with an emphasis on specialties, to provide the recent and advanced treatment.

― 14 ―

Oral Health Sciences

Oral Health Sciences(2)

Publications[Original Articles]
1. Tomohiro Kohmoto, Atsumi Tsuji, Kei-Ichi Morita, Takuya Naruto, Kiyoshi Masuda, Kenichi Kashimada,
Keisuke Enomoto, Tomohiro Morio, Hiroyuki Harada, Issei Imoto. A novel COL11A1 missense mutationin siblings with non-ocular Stickler syndrome. Hum Genome Var. 2016; 3; 16003
2. Kou Kayamori, Ken-Ichi Katsube, Kei Sakamoto, Yoshio Ohyama, Hideaki Hirai, Akane Yukimori, Yae
Ohata, Takumi Akashi, Masao Saitoh, Kiyoshi Harada, Hiroyuki Harada, Akira Yamaguchi. NOTCH3 Is
Induced in Cancer-Associated Fibroblasts and Promotes Angiogenesis in Oral Squamous Cell Carcinoma.
PLoS ONE. 2016; 11(4); e0154112
3. Kondo Y, Ohno T, Nishii N, Harada K, Yagita H, Azuma M. Diﬀerential contribution of three immune
checkpoint (VISTA, CTLA-4, PD-1) pathways to antitumor responses against squamous cell carcinoma.
Oral Oncology. 2016; 57; 54-60
4. Kondo Y，Ohno T，Nishii N，Harada K，Yagita H，Azuma M. Diﬀerential contribution of three immune
checkpoint (VISTA, CTLA-4, PD-1) pathways to antitumor responses against squamous cell carcinoma
Oral Oncology. 2016; 57; 54-60
5. Masaru S，Morita K，Kabasawa Y，Harada H. Bilateral nasolabial cysts: a case report Jounal of Medicalcase Reports. 2016; 10; 246
6. Mochizuki Y，Tomioka H，Tsushima F，Shimamoto H，Oikawa Y，Harada H. Clinical evaluation of thecoverage of open wounds in the oral surgical ﬁeld with a polyglycolic acid sheet and a ﬁbrin glue sprayand coverage with split thickness skin grafting Annals Maxillofacial Surgery. 2016; 6(2); 228-234
7. Kato Y，Ogasawara S，Oki H，Honma R，Takagi M，Fujii Y，Nakamura T，Saidoh N，Kanno H，Umetsu
M，Kamata S，Kubo H，Yamada M，Sawa Y，Morita K，Harada H，Suzuki H，Kaneko MK. Novel
Monoclonal Antibody LpMab-17 Developed by CasMab Technology Distinguishes Human Podoplaninfrom Monkey Podoplanin Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 2016; 2(35);109-116
8. Masanori Kudoh, Ken Omura, Arata Satsukawa, Koshi Matsumoto, Takahide Taguchi, Hiroyuki Harada,
Yoshimasa Ishii. Symmetric Lipomatosis Arising in the Tongue Presenting as Macroglossia and Articulatory Disorder Case Reports in Otolaryngology. 2016; (2016);
9. Nguyen CT，Okamura T，Morita K，Yamaguchi S，Harada H，Miki Y，Izumo T，Kayamori K，Yamaguchi
A，Sakamoto K. LAMC2 is a predictive marker for the malignant progression of leukoplakia Journal of
Oral Pathology and Medicine. 2016;
10. Ohata Y，Tohyama R，Kayamori K，Shimamoto H，Hirota Y，Harada H，Izumo T，Sakamoto K. A
rare cancer of the tongue that demonstrated unusual histology and immunohistochemical phenotype: casereport International Dentistry Scientiﬁc Meeting 2016/UI Proceedings on Health and Medicine. 2016;
1(1); 172-175
11. Oda S，Nozawa T，Nozawa-Minowa A，Tanaka M，Aikawa C，Harada H，Nakagawa I. Golgi-Resident
GTPase Rab30 Promotes the Biogenesis of Pathogen-Containing Autophagosomes PLoS One. 2016.01;
11(1); e0147061
12. Shirakawa J，Harada H，Noda M，Ezura Y. PTH-Induced Osteoblast Proliferation Requires Upregulationof the Ubiquitin-Speciﬁc Peptidase 2 (Usp2) Expression Calciﬁed Tissue International. 2016.03; 98(3);306-315
13. Tohyama R，Kayamori K，Sato K，Hamagaki M，Sakamoto K，Yasuda H，Yamaguchi A. Establishment ofa xenograft model to explore the mechanism of bone destruction by human oral cancers and its application
to analysis of role of RANKL Journal of Oral Pathology & Medicine. 2016.05; 45(5); 356-364
14. Macedo R，Rochefort J，Guillot-Delost M，Tanaka K，Le Moignic A，Noizat C，Baillou C，Mateo V，Carpentier AF，Tartour E，Bertolus C，Bellier B，Lescaille G，Lemoine FM. Intra-cheek immunization
as a novel vaccination route for therapeutic vaccines of head and neck squamous cell carcinomas using
plasmo virus-like particles Oncoimmunology. 2016.07; 5(7); e1164363― 15 ―

Oral Health Sciences

Oral Health Sciences

15. Marukawa E，Tamai M，Takahashi Y，Hatakeyama I，Sato M，Higuchi Y，Kakidachi H，Taniguchi
H，Sakamoto T，Honda J，Omura K，Harada H. Comparison of magnesium alloys and poly-l-lactide
screws as degradable implants in a canine fracture model Journal of Biomedical Materials Research Part
B: Applied Biomaterials. 2016.10; 104(7); 1282-1289[Conference Activities & Talks]
1. Marukawa E, Harada H, Sasaki Y, Akiyoshi K. Osteoinduction using β-TCP with Nanogel-crosslinking
hydrogel as Nobel scaﬀord for BMP-2. Academy of Osseointegration’s 31st Annual Meeting, February
17-20 2016. 2016.02.17 San Diego
2. Marukawa E, Stricker A, Fleiner J, Flugge TV, Harada H, Schmelzeisen R, Nelson K. The diﬀerence
in bone resorption between mandibular ramus bone block graft and bone splitting for alveolar ridge
augmentation. Academy of Osseointegration’s 31st Annual Meeting, February 17-20 2016. 2016.02.17 San
Diego
3. Morita K, Naruto T, Tanimoto K, Yasukawa C, Oikawa Y, Masuda K, Imoto I, Inazawa J, Omura K,
Harada H. Simultaneous detection of both single nucleotide variations and copy number alterations by
next-generation sequencing in gorlin syndrome. The 13th International Congress of Human Genetics,
April 3-7 2016 2016.04.03 Kyoto
4. Nishii N, Kondo Y, Li L, Lau W, Hi, Harada H, Azuma M. Combined treatment with PD-L1 blockade
and a TLR7/8 agonist dramatically enhances antitumor immunity. AACR2016 2016.04.16 New Orleans,
LA, USA Ernest N. Morial Convention Center
5. Takahara N, Kabasawa Y, Tetsumura A, Kurabayashi T, Harada H. Dynamic MR imaging of the temporomandibular joint: a preliminary study. Advanced Digital Technology (ADT) North America & Japan
Regional Leadership Groups Conference,June 16-20 2016. 2016.06.16 San Diego
6. Kabasawa Y, Tsuchida Y, Takahara N, Kamijo S, Suzuki T. :Accuracy of a 3D Model for Surgical Supportin Orthognathic Surgery- Eﬃcacy of Data Synthesis with Computed Tomography and Dental Model Scan. Advanced Digital Technology (ADT) North America & Japan Regional Leadership Groups Conference,
June 16-20 2016. 2016.06.16 San Diego
7. Miyazaki H，Romeo U，Wada Y，Kato J，Tanaka K，Alessandro DV, Maruoka Y， Watanabe H，Harada H，Asamura S. Treatment strategies for large oral vascular anomalies using intralesional laser
photocoagulation. The 15th Congress of the World Federation for Laser Dentistry, July 17-19 2016.
2016.07.17 Aichi― 16 ―

Oral
Oral Health
Health Sciences
Sciences

Oral and Maxillofacial Radiology

Professor: Tohru KURABAYASHI
Associate Professor: Hiroshi WATANABE
Junior Associate Professor: Naoto OHBAYASHI, Norio YOSHINO
Assistant Professor: Akemi TETSUMURA, Shin NAKAMURA, Ami KURIBAYASHI, Junichiro SAKAMOTO
Hospital Staﬀ: Yoshikazu NOMURA, Mamiko FUJIKURA
Graduate Student: Shinya KOTAKI, Ngamsom SUPAK, Hiroko ISHII, Noriko SUZUKI,
Chutamas DEEPHO, Tran Thi Xuan LAN, Sakurako ASAI, Wamasing PEERAPONG
Research Student: Sakurako ASAI, Shintarou TAKADA
Secretary: Izumi MOTOHASHI(1)

Research

1) Diagnosis of maxillofacial diseases by CT, MRI and PET imaging
2) Advantages of cone-beam CT for clinical dentistry3) Development of high resolution MRI technology.
4) Novel MRI techniques for TMJ disorders.
5) Factors determining radioresistance of oral and maxillofacial cancers.

(2)

Lectures & Courses

Oral and maxillofacial radiology is a branch of dental science which deals with the eﬀective application ofradiation energy to the diagnosis and treatment of oral and maxillofacial diseases. Main objective of oral andmaxillofacial radiology in the graduate course is to provide students opportunity to study advanced imaging
modalities including digital imaging, cone-beam CT, multi-detector row CT and MRI, and also to study image
processing and image analysis technology. Students are also taught on basic radiation oncology and its related
laboratory technology depending on their research project.
(3)

Clinical Services & Other Works

Oral and maxillofacial radiology clinic provides a full spectrum of imaging examinations and diagnosis, including
CT and MRI. Non-invasive, interventional radiology for patients with salivary gland stone is also performed in
the clinic.
(4)

Publications[Original Articles]
1. Yoshi S, Sumita Y, Hattori M, Ohbayashi N, Kurabayashi T, Taniguchi H. The morphological evaluationof denture space in glossectomy patients. Maxillofacial Prothetics. 2016; 39; 60-67
2. Sakamoto J, Kuribayashi A, Kotaki S, Fujikura M, Nakamura S, Kurabayashi T. Application of Diﬀusion
Kurtosis Imaging to Odontogenic Lesions: Analysis of the Cystic Component J Magn Reson Imaging.
2016.12; 44(6); 1565-1571― 17 ―

Oral
HealthSciences
Oral
HealthSciences

3. Kotaki S, Sakamoto J, Kretapirom K, Supak N, Sumi Y, Kurabayashi T. Diﬀusion tensor imaging of theinferior alveolar nerve using 3T MRI: a study for quantitative evaluation and ﬁbre tracking. Dentomaxillofac Radiol. 2016.12; 45(8); 20160200
4. Atsushi Kaida, Hiroshi Watanabe, Kazuma Toda, Keiko Yuasa-Nakagawa, Ryoichi Yoshimura, Masahiko
Miura. Eﬀects of dose rate on early and late complications in low dose rate brachytherapy for mobile
tongue carcinoma using Ir-192 sources. Oral Radiol. 2016.12; published online;
5. Uraba S,Ebihara A,Komatsu K,Ohbayashi N,Okiji T. Ability of cone-beam computed tomography todetect periapical lesions that were not detected by periapical radiography: A retrospective assessment
according to tooth group Journal of Endodontics.
[Conference Activities & Talks]
1. Nakamura S. Imaging diagnosis of malignant lymphoma. The 38th Annual Scientiﬁc Conference on Dental
Research 2016.04 Ho-Chi-Minh City, Vietnam
2. Kotaki S, Sakamoto J, Kurabayashi T. MRI ﬁndings of the inferior alveolar nerve. The 38th Annual
Scientiﬁc Conference on Dental Research. April 5th, 2016, Ho-Chi-Minh City, Vietnam 2016.04.05
3. Ngamsom S, Nakamura S, Sakamoto J, Kotaki S, Kurabayashi T. Quantitative evaluation of lateralpterygoid muscles in patients with temporomandibular joint disorders by intravoxel incoherent motion
MRI . Swiss Congress of Radiology 2016 2016.05 Davos, Switzerland
4. Ozawa E, Honda E, Ohmori H, Shimazaki K, Kurabayashi T, Ono M. Inﬂuence of orthodontic appliancederived artifacts on 3-T MRI movies. IADR/APR 94th General Session 2016.06.22 Seoul
5. Watanabe C, Wada J, Mizutani K, Watanabe H, Wakabayashi N. The eﬀect of removable partial denture
placement on periodontal structures of abutment teeth in patients of periodontitis: A digital subtraction
analysis. The 125th Scientiﬁc Meeting of Japan Prosthodontic Society 2016.07.10 Kanazawa
6. Nguyen Ho Q, Yamazaki Y, Imura H, Yoshino N, Kurabayashi T, Taira M, Shimada M. Peripheral injuries
may lead to grey matter volume reduction in post traumatic trigeminal neuropathic pain. 16th World
Congress of Pain 2016.09.26 Yokohoma
7. Nakajima Y, Iwasaki N, Takahashi H, Yoshida M, Honda E, Kurabayashi T. MRI artifacts and radiopacityof CAD/CAM composite resin blocks. The 2016 Academy of Dental Materials Annual Meeting 2016.10.12
Chicago8. Nakamura S, Sakamoto J, Yoshino N, Kurabayahi T. Imaging of lingual lymph node metastasis in patientswith squamous cell carcinoma of the tongue . The 11th Asian Congress of Maxillofacial Radioloy 2016.11
Chiang Mai, Thailand
9. Watanabe H, Ozaki Y, Miura M, Yoshimura R, Sumi Y, Kurabayashi T. Estimation of whole bodyradiation exposure induced by oral cancer brachytherapy using Monte Carlo simulation. The 11th AsianCongress of Oral and Maxillofacial Radiology 2016.11.12 Chiang Mai, Thailand
10. Deepho C, Watanabe H, Kotaki K, Sakamoto J, Sumi Y, Kurabayashi T. Utility of CT/MRI fusion
volumetric images for localizing the mandibular canal. The 11th Asian Congress of Oral and Maxillofacial
Radiology 2016.11.12 Chiang Mai, Thailand
11. Kotaki S, Sakamoto J, Kretapirom K, Ngamsom S, Kurabayashi T. Diﬀusion Tensor Imaging of theInferior Alveolar Nerve Using 3T MRI. The 11th Asian Congress of Oral and Maxillofacial Radiology.
November 12th, 2016, Chiang Mai, Thailand 2016.11.12
12. Toriihara A, Sakamoto J, Nakamura S, Oyama J, Kurabayashi T, Tateishi U. All about the mandible:
chewing and absorbing key points for evaluating morphological and functional images. Radiological Societyof North America 2016 Annual Meeting 2016.11.27 Chicago[Patents]
1. Method of Predicting Metastasis of Oral Cavity Cancer into Cervical Lymph Node and Diagnostic Kit to
be used in the Prediction, Patent Number：8129122 United States Patent― 18 ―

Oral
Oral Health
Health Sciences
Sciences

Anesthesiology and Clinical Physiology

Professor
Haruhisa Fukayama
Associate Professor
Ryo Wakita
Junior Associate Professor
Keiko Abe
Assistant Professors
Tomoyuki Miyamoto, Tomoka Matsumura, Kazumasa Kubota,
Hospital Staﬀs
Yukiko Baba (Jan∼), Kanako Saji (Apr∼),Toru Yamamoto (Apr∼),Keiko Abe (Apr∼),Kanji Doushita (Apr∼), Sayumi Abe (Apr∼)
Graduate Students
Takutoshi Inoue, Kaeko Araki, Asami Izumida,Chihiro Suzuki,
Takaya Itou (Apr∼), Yuu Satou (Apr∼), Tamae Hasegawa (Apr∼)
Research Students
Tsunataka Abo (Apr∼), Hiroyuki Tojo (Apr∼),Akira Oshima (Apr∼), Yosuke Yamada (Apr∼)
Advanced Clinical Training
Feng Yu Pin (Jun∼)
Secretary
Natsu Sato
(1)

OutlineFor safety and comfortable dentistry for both patients and dentists, educations, researches and clinical practices
are performed in the department. Education includes basics and pracitices of local and general anesthesia,
sedation, monitoring (monitored anesthesia care, MAC)in addition to cardio-pulmonary resuscitation, or, basic
life support during dental treatment. Our researches are consisted of basic and clinical trials for the purposes.
Many cases give us many chances to keep the patients safe and comfortable during local and general anesthesia.
Local groups, such as dental associations, are welcomed to promote safe and comfortable dental treatments.
(2)

Research

１）Non-invasive drug delivery system
２）New methods for local anesthesia in dentistry３）Neuropathic pain in oral and maxillofacial regions４）Diﬀuse noxious inhibitory control or controlled pain modulation
５）Sedation for dentistry(3)

Education

Anesthesia and anesthesiology for dentisry, which are not only local anesthesia but also general anesthesia
are given to the both under and post graduate students. Lectures and trainings are consisted of local and― 19 ―

Oral
HealthSciences
Oral
HealthSciences

general anesthesia, sedation and cardio-pulmonary-resuscitation (CPR), or, basic life support (BLS). For local
anesthesia, the students learn mechanism of local anesthesia, local anesthetics, techniques and local and systemic
comoplications due to local anesthesia. Physiology, biochemistry and pharmacology are also provided for generalanesthesia which indludes possible mechanism of general anesthesia, anesthetics, muscle reluxants and what areused for general anesthesia. They also acquire the techniques of topical, inﬁltration and conductions anesthesia,
nitrous oxide inhalation sedation and basic life support.
(4)

Lectures & Courses

Anesthesia and anesthesiology for dentisry, which are not only local anesthesia but also general anesthesia
are given to the both under and post graduate students. Lectures and trainings are consisted of local andgeneral anesthesia, sedation and cardio-pulmonary-resuscitation (CPR), or, basic life support (BLS). For local
anesthesia, the students learn mechanism of local anesthesia, local anesthetics, techniques and local and systemic
comoplications due to local anesthesia. Physiology, biochemistry and pharmacology are also provided for generalanesthesia which indludes possible mechanism of general anesthesia, anesthetics, muscle reluxants and what areused for general anesthesia. They also acquire the techniques of topical, inﬁltration and conductions anesthesia,
nitrous oxide inhalation sedation and basic life support.
(5)

Clinical Services & Other Works

Safe medical and perioperative mangements are give to the patients of our amulatory anesthesia service which
has more than 2,000 cases per year and the central operation rooms which has 750 cases per year. Some diﬃcultcases are referred to our hospital because of many clinical experiences.
Several cases that need emergency care also supported by our department. ER members are sometimes calledin the medical hospital.
Local groups like dental associations often ask us to hold some lectures, trainings, workshops for safe dental
treatment. These proposals are welcomed by our staﬀs. When intravenous sedation case are introduced, some
responsible staﬀ is sent to their own clinic.
(6)

Clinical Performances

Any patient is welcomed, especially patients of oral surgery and implant operation who need special care using
genearl anesthesia and sedation.
Referred patients from open practitioners are also accepted for safe and comfortable dental treatment.
(7)

Publications[Original Articles]
1. Inoue T, Sugiyama T, Ikoma T, Shimazu H, Wakita R, Fukayama H. Drug delivery and transmission oflidocaine using iontophoresis in combination with direct and alternating currents Journal of Medical and
Dental Sciences. 2016; 63(4); 71-77
2. Sasaki Y, Kato S, Miura M, Fukayama H. Correlation between body movements and salivary secretion
during sedation Anesthesia Pgogress. 2016; 63; 185-191
3. Fjii-Abe K, Kawahara H, Fukayama H. An analysis of green discoloration of urine caused by propofol
infusion Journal of Clinical Anesthesia. 2016; 35; 358-360
4. Matsumura T, Kubota K, Sakamoto K, Fukayama H. NGF is induced bylidocaine in human skin ﬁbroblast
cell in vitro Masui. 2016.03; 65; 288-290[Books etc]
1. Kazuhiro Shimoyama, Haruhisa Fukayama. Dictionary of Gerodontology. Ishiyaku Publishers, INC.,
2016.03 (ISBN : 978-4-263-45793-1)― 20 ―

Oral
Oral Health
Health Sciences
Sciences[Conference Activities & Talks]
1. Ryo Wakita and Haruhisa Fukayama. . Waveform of End-tidal Carbon Dioxide is useful to detect
hypopnea during intravenous sedation. 36th Myamnar Dental Conference 2016.01 Yangon
2. Haruhisa Fukayama. Education of anesthesia in dentistry. 36tn Myanmar Dental Conference & 17th
FDI-MDA joint Educational Meeting 2016.01.28 Yangon, Myanmar
3. Haruhisa Fukayama. Safe Management of Medically Compromised Patients - How can we manage thepatients ? -. The 38th Annual Scientiﬁc Conference on Dental Research 2016.04.05 Ho Chi Minh City,
Vietnam
4. Ryo Wakita and Haruhisa Fukayama.. Emergency response in Tokyo Medical and Dental University
Dental Hospital. -Two years statistical reports-. 7th Mandaley Dental Conference and Mid-year Meetingof Myanmar Dental association, 2016.07 Mandaley
5. Haruhisa Fukayama. Sedation for persons with disabilities. The 20th General Assembly, the 39th Annual
Scientiﬁc Meeting and Dental Exhibition of the Association for Dental Sciences of the Rebublic of China
2016.09.02 Taipei, Taiwan
6. Transient ST-segment elevation and decreased blood pressure in a patient under sedation after an oral
surgery: A case report.. 2016.10.29
7. Medical emergencies in 14,671 invasive dental treatment cases of medically compromised elderly patients.
2016.10.29[Social Contribution]
1. BLS training course in MDA conference, Myanmar Dental Association, Yangon, 2016.01.30― 21 ―

Oral Health Sciences

Oral Health Sciences

Orofacial Pain Management

Professor
Masahiko SHIMADA
Junior Associate Professor
Akira NISHIYAMA
Assistant Professor
Yoko YAMAZAKI
Hospital Staﬀ
Hiroko KIMURA, Daisuke TOMIZAWA、Hiroko IMURA, Masako TOBE,
Hiroyuki ISHIYAMA, Maya SAKAMOTO
Graduate Student
Akitoshi HOSODA, Kaori TUKAGOSHI、Rena NAKAYAMA,
Nguyen Ho QUYNH ANH Ngan Nguyen, Liang Shanshan(1)OutlineMain research subjects of orofacial pain management is to establish the diagnosis and treatment of the disease
with a pain, abnormal sensation, sensory paralysis, abnormal movement, motor paralysis and temporomandibular disorders , in particular, is to elucidate the mechanism of pain, neuropathic pain, temporomandibular disorders.

(2)

Research

Main research subjects of orofacial pain management is to establish the diagnosis and treatment of the disease
with a pain, abnormal sensation, sensory paralysis, abnormal movement, motor paralysis and temporomandibular disorders , in particular, is to elucidate the mechanism of pain, neuropathic pain, temporomandibular disorders.
1)New Treatment methods for neuropathic pain2)Analyses of abnormal orofacila pain
3)Study on Biological Response to Dental Interventions4)Analyses and new treatment of dysgeusia5)Development of multidimensional evaluation system for etiological factors of TMD
6)Inﬂuence of patients psychosomatic factors for TMD
7)Sleep bruxism: its etiology, inﬂuence and treatment8)Eﬀectiveness of physiological therapy for TMD9)Mechanisms of occlusal discomfort(3)

Education

Purpose of education for students and residents in this course is to provide an opportunity to learn basic
knowledge on diagnostic and treatment of the disease with a pain, abnormal sensation, sensory paralysis,
abnormal movement, motor paralysis and temporomandibular disorders in the orofacial area. In special course
for graduate students, main objective of orofacial pain management is to learn the diagnosis and treatmentof the disease with a pain, abnormal sensation, sensory paralysis, abnormal movement, motor paralysis andtemporomandibular disorders in the orofacial area, in particular, mechanism of pain, neuropathic pain,
temporomandibular disorders, and we instruct statistical techniques especially with the multi variate analysis
by using clinical data acquired from patients with temporomandibular disorders (TMD).

― 22 ―

Oral Health Sciences

Oral Health Sciences(4)

Clinical Services & Other Works

Orofacial Pain Clinic is concerned with the the pain, abnormal sensation, sensory paralysis, abnormal movement, and motor paralysis in the orofacial area and management of orofacial pain clinic is pharmacotherapy, nerve block, stimulation of the pheripheral nerves including acupuncture and psychotherapies. Temporomandibular joint clinic provides diagnosis and treatment for diseases and disfunctions of temporomandibular
joint and masticatory muscles. We also provide the treatments for the nocturnal bruxism and the occlusal
discomfort.
(5)

Clinical Performances

Orofacial Pain Clinic is concerned with the the pain, abnormal sensation, sensory paralysis, abnormal movement, and motor paralysis in the orofacial area and management of orofacial pain clinic is pharmacotherapy, nerve block, stimulation of the pheripheral nerves including acupuncture and psychotherapies. Temporomandibular joint clinic provides diagnosis and treatment for diseases and disfunctions of temporomandibular
joint and masticatory muscles. We also provide the treatments for the nocturnal bruxism and the occlusal
discomfort.
(6)

Publications[Original Articles]
1. Imura Hiroko, Shimada Masahiko, Yamazaki Yoko, Sugimoto Kumiko. Characteristic changes of saliva
and taste in burning mouth syndrome patients Jouranal of Oral Pathology and Medicine. 2016.03; 45(3);231-236
2. Nishiyama A, Tsuchida E. Relationship Between Wind Instrument Playing Habits and Symptoms of
Temporomandibular Disorders in Non-Professional Musicians The Open Dentistry Journal. 2016.08; 10;411-416[Conference Activities & Talks]
1. Ayako Kubota, Yoko Kono, Hiroko Imura, Hiroji Shimomura, Kiyoko Kanamori, Yasuko Kawakami, Shiro
Mataki, Kumiko Sugimoto. Oral health status and behavior of Japanese university students. International
Symposium on Dental Hygiene 2016 2016.06 Basel, Switzerland
2. Hiroyuki Ishiyama, Shusuke Inukai, Akira Nishiyama, Masayuki Hideshima, Shuhei Nakamura, Chisato
Ida, Yuko Mitsuma, Shota Hayashi, Akihito Uesato, Toshihide Fujie, Meiyo Tamaoka, Yasunari Miyazaki,
Masahiko Shimada, Noriyuki Wakabayashi . Eﬀect of jaw-opening exercise on prevention of temporomandibular disorders pain associated with oral appliance therapy and compliance in obstructive sleep
apnea patients: A randomized, double-blind, placebo-controlled trial.. Japanese Society of Sleep Researchthe 41th Annual Meeting 2016.07.07 Tokyo
3. Tomoko Niimi, Yoko Yamazaki, Masato Kawashima, Daisuke Tomizawa, HIroko Imura, Akitoshi Hosoda,
Masahiko Shimada. Eﬀectiveness of Rikkosan for Intraoral Intractable Pain. IASP 2016.09.27 Yokohama
4. Nguyen Ho Quynh Anh, Yamazaki Yoko, Imura Hiroko, Taira Masato, Kurabayashi Tohru, Shimada
Masahiko. ERIPHERAL INJURIES MAY LEAD TO GREY MATTER VOLUME REDUCTION IN
POST TRAUMATIC TRIGEMINAL NEUROPATHIC PAIN. IASP 2016.09.28 Yokohama
5. Nguyen Ho Quynh Anh, Yamazaki Yoko, Imura Hiroko, Norio Yoshino ,Taira Masato, Kurabayashi Tohru,
Shimada Masahiko. TRIGEMINAL NEURALGIA AND THE BRAIN PLASTICITY. The 81th Annual
Meeting of The Stomatological Society 2016.11.26 Tokyo― 23 ―

Oral Health Sciences

Oral Health Sciences

Pediatric Dentistry

Associate Professor
Michiyo MIYASHIN
Assistant Professor
Yoshiaki HASHIMOTO, Haruko FUJITA(∼Mar.), Mizuho MOTEGI,Satoko KAKINO, Tomoki UEHARA(Aug.∼)
Clinical Professor
Keiichi TAKEI
Adjunct Lecturer
Hitoyata SHIMOKAWA, Mitsuko INOUE, Masayo ONO(Apr.∼),Hiroaki NAGAI, Nobutaka ISOGAWA,
Asuri jayawarudeina(Apr.∼)Yuki IMAMURA(∼Mar.), Naoko UEHARA, Makiko TAKASHI,
Natsumi TSUCHIHASHI(Apr.∼), Sachiko ITOH(Apr.∼）Syuko MURATA(∼Mar.)
Hospital staﬀ
Yukie Nakajima, Atsushi OISHI, Sachi GOTOH,
Taki KEKIYA, Kanae WADA, Sachiko ITOH(∼Mar),
Tomoki UEHARA(Apr.∼Jul.)
Graduate Student
Tomoki UEHARA(∼Mar.), Kuniomi NAKAMURA,
SHEN Dong He, GANBOLD Khongorzul, Erika KUBOTA,
IJBARA Manhal M.A., ZUMULAITI Shaokelati,
WIT Yee Wint(Oct.∼), Satomi FURUSAWA,
Shigeki NAGAHIRO(Apr.∼)Research Student
Mami AOKI(∼Mar.), Miki IIDA(∼Mar.), Anri OHTA(∼Mar.),Chikako SATOH(∼Mar.), Aoi AKAIKE, Chika INOHARA,
Maya KATAOKA(∼Sep.), Kenichi MIURA(Apr.∼)Enrolled dentist
Ayako TOKUDA, Gaku SHIMADA(Sep.∼), Tomoko KAWAMURA(May.∼)
Yui SEKIDO(Aug.∼), Mana KATAOKA(Nov.∼)(1)

OutlineThe Department of Pediatric Dentistry was founded in 1955, as the ﬁrst in Japan. Pediatric dentistry is a subjectof clinical dentistry that deal with education and research of not only developmental oral health sciences but also

― 24 ―

Oral Health Sciences

Oral Health Sciences

prevention and treatment methods of the diseases which disturb oro-facial growth and development of children.
(2)

ResearchResearch Subjects
1) Physiological and biological studies on the stomatognathic function of children
2) Studies on the development and developmental disturbance of the teeth
3) Studies on the growth and development of the maxillofacial cranium and the dentition
4) Development of new Endodontics and Traumatology for deciduous and immature permanent teeth5) Basic research on clinical pediatric dentistry(3)

Education

Lecture subjects
Pediatric dentistry, Oral pediatrics(4)

Lectures & Courses

The main objective of pediatric dentistry in this graduate course is to provide students an opportunity to study
the theory and the method for the guidance of the oro-facial growth and development and for the diagnosis,prevention and treatment of diseases and malfunctions which disturb the oro-facial growth and development
during the period of childhood.
Oral pediatrics is a subject of clinical dentistry that deal with education and research of not only maintenanceand promotion of the oral health for growing children but also prevention and treatment methods of diseases
and malfunctions which disturb oral health of growing children. The main objective of oral pediatrics in thisgraduate course is to provide students an opportunity to understand that a child is a living body with mental,
physical, and physiological characteristics which are diﬀerent from those of adults and to study the pathogenesis,
prevention, and treatment of the particular oral diseases in childhood. Students are also taught the theory andthe method of ongoing health care that is necessary for maintaining and promoting oral health from infant toadult. In addition, they are taught the clinical signiﬁcance and importance of the behavioral management ofchild patients and the necessity and importance of understanding and cooperation of the parents to it.
(5)

Clinical Services & Other Works

The pediatric dentistry clinic in the department of oro-facial development and function provides the comprehensive dental treatment for a child while growing.
(6)

Clinical Performances

The examination, diagnosis, and treatment of the oral diseases and the oral abnormalities are performed in theclinic. In addition, health guidance, preventive measures, and the long-term oral health management by theperiodical checking system are carried out, in order to keep and promote oral health from infant to adult.
(7)

Publications[Original Articles]
1. Wada K, Ikeda E, Wada J, Inoue G, Miyasaka M, Miyashin M. Wear characteristics of trimethylolpropane
trimethacrylate ﬁller-containing resins for the full crown restoration of primary molars. Dental Materials
Journal. 2016; 35(4); 585-593
2. Yamada A, Kakino S, Matsuura Y. Photoacoustic detection of blood in dental pulp by using short-time
Fourier transform. Proceeding of SPIE. 2016.02; 9708-102
3. Tanaka S, Uehara N, Tsuchihashi N, Sugimoto K. Emotional relationships between child patients andtheir mothers during dental treatments. Journal of Dental Sciences. 2016.04; 11; 287-292― 25 ―

Oral Health Sciences

Oral Health Sciences

4. Sasagawa I, Oka S, Mikami M, Yokosuka H, Ishiyama M, Imai A, Shimokawa H and Uchida U. Immunohistochemical and Western blot analyses of ganoine in the ganoid scales of Lepisosteus oculatus, anactinopterygian ﬁsh. J. Exp. Zool (Mol. Dev. Evol). 2016.05; 326B(3); 193-209
5. Uehara T, Mise-Omata S, Matsui M, Tabata Y, Murali R, Miyashin M, Aoki K. Delivery of RANKLBinding Peptide OP3-4 Promotes BMP-2-Induced Maxillary Bone Regeneration. J. Dent. Res. 2016.06;
95(6); 665-672
6. Ito S, Shimada Y, Sadr A, Nakajima Y, Miyashin M, Tagami J, Sumi Y. Assessment of occlusal ﬁsure
depth and sealant penetration using optical coherence tomography. Dental Materials Journal. 2016.06;
35(3); 432-433
7. Seko Y, Azuma N, Yokoi T, Kami D, Ishii R, Nishina S, Toyoda M, Shimokawa H, Umezawa A. Anteroposterior Patterning of Gene Expression in the Human Infant Sclera: Chondrogenic Potential and Wnt
Signaling. Curr Eye Res. 2016.06; 1-10
8. Yamada A, Kakino S, Matsuura Y. Detection of Photoacostic Signals from Blood in Dental Pulp. Optics
and Photonics Journal. 2016.09; 6; 229-236
9. Taki Sekiya, Hiroko Imura, Sachiko Ito, Michiyo Miyashin, Kumiko Sugimoto. Development of real-time
stress monitoring system in pediatric dentistry: Assessment from autonomic nerve and brain activities
2016.11; 54(4); 443-449[Conference Activities & Talks]
1. Tamura Y, Mise S, Sugamori Y, Bhuyan MZH, Takahashi M, Uehara T, Arai Y, Miyashin M, Wakabayashi
N, Aoki K. A pharmacological role of metallothionein in zinc-treated cartilage-progenitor cells. The 13th
Asia Paciﬁc Federation of Pharmacologists Meeting 2016.02.01 Bangkok
2. Yamada A, Kakino S, Matsuura Y. Photoacoustic detection of blood in dental pulp by using short-time
Fourier transform. Biomedical Optics Conference (BiOS), SPIE 2016.02.16 San Francisco, USA
3. Sugawara Y, Kashimada A, Moriyama K, Baba S, Imai K, Nishikomori R, Motegi M, Takeuchi Y, Morio
T. Aicardi-Goutieres and Singleton-Merten overlapping phenotype due to IFIH1 mutation.. The 13th
International Congress of Human Genetics 2016.04.06 Kyoto, Japan
4. Oishi A, Shimokawa H, Wit Yee Wint, Arakawa S, Miyashin M. Eﬀects of Nanobubble water on humancells derived from impacted supernumerary teeth. 10th Biennial Conference of the Pediatric Dentistry
Association of Asia in conjunction with 54th Annual Conference of the Japanese Society of Pediatric
Dentistry 2016.05.27 Tokyo, Japan
5. Gotoh S, Fujita H, Shimokawa H, Miyashin M. A Case of Hyper-IgE Syndrome, Dental Treatment andHistologic Examinations of a Deciduous Tooth. 10th Biennial Conference of the Pediatric Dentistry
Association of Asia 2016.05.27 Tokyo, Japan
6. Ganbold K, Kakino S, Matsuura Y, Matoba K, Miyashin M. Relevance of pulpal blood ﬂow and root
development of young permanent teeth measured by Transmitted-light Plethysmography (TLP). 10th
Biennial Conference of the Pediatric Dentistry Association of Asia 2016.05.27 Tokyo
7. Ijbara M, Wada K, Miyashin K. Simulated Occlusal Wear In Immature Teeth: 3D Analysis. 10th Biennial
Conference of the Pediatric Dentistry Association of Asia 2016.05.28 Tokyo, Japan
8. Kakino S, Ganbold K, Miyashin M. Pulpal circulation in developing healthy and traumatized teeth using TLP. 19th World Congress on Dental Traumatology and 5th Trans-Tasman Endodontic Conference
2016.08.11 Brisbane, Australia
9. Nakamura K, Oishi A, Nakajima Y, Shibata S, Miyashin M. Longer-term follow-up data of experimental
traumatic dislocations and root fractures. The 23rd General Meeting of the Japanese Association for
Dental Science 2016.10.21 Fukuoka[Awards & Honors]
1. Poster Presentation, Second Place Award, International Association for Dental Traumatology, 2016.08― 26 ―

Oral
Oral Health
Health Sciences
Sciences

Orthodontic Science

Professor
Associate Professor
Junior Associate Professor
Assistant Professor

Takashi ONO

Yoshiro MATSUMOTO, Zuisei KANNO, Jun HOSOMICHI
Kazuo SHIMAZAKI, Ippei WATARI, Satoshi KOKAI, Ikuo YONEMITSU
Takayoshi ISHIDA
Project Assistant Professor (International Exchange Center)
Yuji ISHIDA
Dental Resident
Naoki SHIBUTANI (Apr-), Hiroko OMORI(-Mar), Yasuhiro SHIMIZU, Risa USUMI
Chiho KATO, Hidemasa OKIHARA, Yuhei IKEDA (Apr-)
Graduate Students Shuji OISHI (-Mar), Soma KITA (-Mar)
Yoichiro KUMA (-Mar), Tomomi SAKAGUCHI (-Mar), Mio MAKIGUCHI (-Mar)
Hiroyuki YAMAGUCHI (-Mar), Jin-Gyu AN, Wei-Jen LAI (-Sep)
Yasunori ABE, Yukano FUKUSHIMA, Yuki KASAHARA
Takuya OGAWA, Iku SHIBATA, Karin Harumi UCHIMA KOECKLIN
Akemi KANAGUCHI, Velusamy PAVETHY NATH, Eri SAITO
Kayo KIMURA, Yuta NAKAI, Kenzo WATAKABE
Erusu NIN, Huan TANG, Roody BEAUBOEUF
Edward CHO, Masamu INOUE, Erika OZAWA
Moe SATO, Kasumi HATANO, Keiko FUKINO
Shin-Sheng Yang, Lu ZAHO, Thi kim Uyen DONG
Meshari Faleh H ALRESHIDI, Misaki AOYAGI (Apr-)
Yuta UCHIKAWA (Apr-), Akihiro KOYAMA (Apr-), Kim Sun-min (Oct-)
Phyo Thura Aung (Oct-)
Graduate School Research Students Hiroko OMORI (Apr-), Yukiha FUNAKI (-Mar),
Ayako KAWABE (-Mar), Rieko ONO (-Mar), Arisa SAWADA (-Mar),
Emina WAKASUGI (-Mar), Yuhei IKEDA (-Mar), Toshihiro IMAMURA
Minami MIYASAKA, Mutsumi MIYAZAKI, Asuka OKITO
Ayako KIRII, Syusuke UESUGI, Shuji OISHI (Apr-), Soma KITA (Apr-)
Yoichiro KUMA (Apr-), Tomomi SAKAGUCHI (Apr-)
Mio MAKIGUCHI (Apr-), Hiroyuki YAMAGUCHI (Apr-)
Tomonari MATSUMURA, Junpei SUZUKI, Kyohei YAMADA
Misako KOKETSU, Takahiro SHIMAMINE, Katsuhiko SUZUKI
Mirei HAGIWARA, Chiho SATOKAWA, Asuka MANABE
Shuntaro SAWAZAKI, Makiko OKUZAWA, Preska KEO (-Mar)
Xiyuan GUO (-Mar), Wu YANG (-Mar), Sun-min KIM (-Sep)
Masako KAWADA (Apr-), Takashi TANAKA (Apr-)
Shunya HORIUCHI (Apr-), Shams SHAHRIAR MOHD (Apr-)
Haixin HONG (Oct-)
Visiting Research Scholars Jui-Chin HSU, Wei-Jen LAI (Oct-)(1)

OutlineOrthodontic Science is one of the dental sciences which propose to control the craniofacial growth and development in equilibrium with the whole body, and also deals with the prevention and/or treatment of malocclusion― 27 ―

Oral
HealthSciences
Oral
HealthSciencesand related disorders, by which the alteration of maxillofacial function with aging could be kept to the mostsuitable condition.
(2)

ResearchResearch Subjects
1) Biomechanical study of occlusion
2) Studies on biological response and functional adaptation followed by orthodontic and occlusal stimulation
3) Clinical application of autotransplantation in orthodontic treatment
4) Studies on interrelation between malocclusion and temporomandibular joint5) Studies on occlusion and age-related changes in cranio-maxillofacial morphology and function6) Studies on interrelation between cranio-maxillofacial complex and whole body7) Development of mechanics and materials for orthodontic treatment
8) Pathophysiological studies on sleep and breathing disorders
9) Studies on interrelation between breathing and body function(3)

Education

Subjects of Education:
Orthodontic Science, Pathophysiology for Malocclusion, Biology for Functional Adaptation(4)

Lectures & Courses

Orthodontic Science
1) To explain the unhealthy physiological condition of malocclusion and deepen the scientiﬁc basis for orthodontic treatment.
2) To understand the biological reaction and adaptation of occlusal tissues to mechanical stresses such as occlusal force or orthodontic force, and also the changes with aging.
3) To explain the art for controlling the morphologic and functional problems of occlusion in orthodontic treatment, from the view points of biomaterials and biomechanics.
4) To enlighten the social dentistry for the needs and demands of orthodontic treatment.
Pathophysiology for Malocclusion
To understand the alteration of occlusal function and morphology with aging, and to explain the pathologicalcondition of malocclusion from the viewpoint of physiology, biomechanics, biology and sociology.
Biology for Functional Adaptation
To understand the procedure of biological reaction and adaptation of occlusal system to the orthodontic stimuli,
including the inﬂuence of aging, and to provide the control of the surroundings of the occlusal system.
(5)

Clinical Services & Other Works

Clinical Services
In the ﬁeld of practical orthodontic, with the development of materials and treatment techniques, we havetaken initiatives in two big turning points at all time. Namely, one is the Direct Bonding System which has
made it possible to attach brackets directly to the teeth surface without orthodontic metal bands. Another isthe development of Super-Elastic Ti-Ni Alloy Wire, and following Improved Super-Elastic Ti-Ni Alloy Wire.
With these new wires, we have provided an epoch-making orthodontic technique, where teeth could be moved
more eﬃciently and safely with light continuous forces, and in consequences, the limits for teeth movement are
expanded and the treatment outcomes are also improved. On the other hand, in order to determine the scientiﬁcbasis for the needs of orthodontic treatment, we are engaging in the study of pathophysiology of malocclusion,and these research results are getting feedback to the orthodontic practices as soon as possible to stimulate thedevelopment of new treatment protocols.
Students in the graduate course not only pursue their scientiﬁc researches but also being educated in accordancewith our curriculum for the post-graduated clinical program. In this program, we aim to bring up the leading― 28 ―

Oral
Oral Health
Health Sciences
Sciences

persons of next generation who have highly specialized knowledge and skills of orthodontics as well as prominent
minds of clinical researches.

(6)

Clinical Performances

Highlights of Clinical Services
1) Orthodontic treatments by using Improved Super-Elastic Ti-Ni Alloy Wire
2) Comprehensive Orthodontic Treatments
With the cooperation of related ﬁeld, we provide comprehensive treatments for those patients with cleft lips andpalates and other congenital anomalies, jaw deformities, maxillofacial functional disorders, periodontal diseases,
impacted teeth, autotransplantation combined cases, and usages of implant anchorages.
(7)

Publications[Original Articles]
1. Oishi S, Shimizu Y, Hosomichi J, Kuma Y, Nagai H, Maeda H, Usumi-Fujita R, Kaneko S, Shitano C,
Suzuki J, Yoshida K, Ono T. Intermittent hypoxia induces disturbances in craniofacial growth and defects
in craniofacial morphology. Arch Oral Biol. 2016.01; 61; 115-124
2. Uesugi S, Yonemitsu I, Kokai S, Takei M, Omura S, Ono T. Features in subjects with the frontal occlusal
plane inclined toward the contralateral side of the mandibular deviation. Am J Orthod Dentofacial Orthop.
2016.01; 149(1); 46-54
3. Miyazaki M, Yonemitsu I, Takei M, Kure-Hattori I, Ono T. The imbalance of masticatory muscle activity
aﬀects the asymmetric growth of condylar cartilage and subchondral bone in rats. Arch Oral Biol. 2016.03;
63; 22-31
4. Abbassy MA, Watari I, Bakry AS, Ono T, Hassan A. Calcitonin and vitamin D3 have high therapeuticpotential for improving diabetic mandibular growth. Int J Oral Sci. 2016.03; 8(1); 39-44
5. Yamada K, Ohmori H, Kirimoto H, Shimazaki H, Ono T. Relation between condylar morphology andtemporomandibular joint sounds in patients with mandibular laterodeviation. Orthod Waves-Jpn Ed.
2016.03; 75(1); 1-7
6. Arai Y, Aoki K, Shimizu Y, Tabata Y, Ono T, Murali R, Mise-Omata S, Wakabayashi N. Peptide-induced
de novo bone formation after tooth extraction prevents alveolar bone loss in a murine tooth extraction
model. Eur J Pharmacol. 2016.04;
7. Tomomi Sakaguchi-Kuma, Nao Hayashi, Hitomi Fujishiro, Kumiko Yamaguchi, Kazuo Shimazaki, Takashi
Ono, Keiichi Akita. An anatomic study of the attachments on the condylar process of the mandible: muscle
bundles from the temporalis Surg Radiol Anat. 2016.05; 38(4); 461-467
8. Maekawa M, Kanno Z, Ono T, Uo M. Development of the all-plastic orthodontic wires. 2016.05; 35(3);189-192
9. Honda K, Omura S, Fujita K, Shibutani N, Yonemitsu I, Shimazaki K, Murata S, Takasu H, Yamashita
Y, Iwai T, Ono T, Tohnai I. A survey of treatment for dentofacial deformities in the past 16 years in oral
and maxillofacial surgery/orthodontics, Yokohama city university medical center. The Japanese Journalof Jaw Deformities. 2016.08; 26(3); 195-201
10. Oishi S, Shimizu Y, Hosomichi J, Kuma Y, Nagai H, Maeda H, Usumi-Fujita R, Kaneko S, Shibutani N, Suzuki J, Yoshida K, Ono T. Intermittent hypoxia Inﬂuences alveolar bone proper microstructure via
hypoxia-inducible factor and VEGF expression in periodontal ligaments of growing rats. Front phsyiol.
2016.09; 7; Article 416
11. Dei A, Miyamoto JJ, Takada JI, Ono T, Moriyama K. Evaluation of blood ﬂow and electromyographic
activity in the perioral muscles. Eur J Orthod. 2016.10;― 29 ―

Oral
HealthSciences
Oral
HealthSciences

12. Kita S, Oshima M, Shimazaki K, Iwai T, Omura S, Ono T. Computational ﬂuid dynamic study of nasal
respiratory function before and after bimaxillary orthognathic surgery with bone trimming at the inferioredge of the pyriform aperture. J Oral Maxillofac Surg. 2016.11; 74(11); 2241-2251
13. Lai WJ, Midorikawa Y, Kanno Z, Takemura H, Suga K, Soga K, Ono T, Uo M. Development and modiﬁcation of a device for three-dimensional measurement of orthodontic force system: The V-bend system
re-visited. Dent Mater J. 2016.12; 35(6); 908-917
14. Makiguchi M, Funaki Y, Kato C, Okihara H, Ishida T, Yabushita T, Kokai S, Ono T. Eﬀects of increased
occlusal vertical dimension on the jaw-opening reﬂex in adult rats. Arch Oral Biol. 2016.12; 72; 39-46
15. Honda K, Omura S, Fujita K, Shibutani N, Yonemitsu I, Shimazaki K, Murata S, Takasu H, Yamashita
Y, Iwai T, Ono T, Tohnai I. A case of mandibular protrusion with excessive vertical maxillary deﬁciency
treated with simultaneous maxillo-mandibular distraction osteogenesis. The Japanese Journal of Jaw
Deformities. 2016.12; 26(4); 275-283
16. Koji Honda, Susumu Omura, Koichi Fujita, Naoki Shibutani, Ikuo Yonemitsu, Kazuo Shimazaki, Shogo
Murata, Hikaru Takasu, Yosuke Yamashita, Toshinori Iwai, Takashi Ono, Iwai Tohnai . A Case ofMandibular Protrusion with Excessive Vertical Maxillary Deﬁciency Treated with Simultaneous Maxillomandibular Distraction Osteogenesis 2016.12; 26(4); 275-283[Conference Activities & Talks]
1. Kasahara Y, Usumi-Fujita R, Hosomichi J, Kaneko S, Ishida Y, Shibutani N, Shimizu Y, Okito A, Oishi S,
Kuma Y, Ono T. Eﬀect of low-intensity pulsed ultrasound on periodontal tissues in occlusal hypofunctional
teeth. The 38th Annual Scientiﬁc Conference on Dental Research 2016.04.04 Ho Chi Minh city, Vietnam
2. Velusamy P, Matin K, Shimada Y, Kanno Z, Ono T, Tagami J. Detection of Hidden Enamel Demineralization beneath Orthodontic Brackets using Optical Coherence Tomography. The 116 th Annual session- American Association of Orthodontists 2016.04.29 Florida, USA
3. Ozawa E, Honda E, Ohmori H, Shimazaki K, Kurabayashi T, Ono T. Inﬂuence of orthodontic appliancederived artifacts on 3-T MRI movies. The 94th General Session & Exhibition of the International Association for Dental Research 2016.05.26 Seoul, Korea
4. Hsu J, Watari I, Ono R, Mizumachi-Kubono M, Funaki Y, Kokai S, Ono T. Degeneration of fungiform
and circumvallate papillae following molar extraction and unilateral nasal obstruction in rats.. The 17th
International Symposium on Olfaction and Taste 2016.06.07 Kanagawa
5. Beauboeuf R, Watari I, Hsu J, Mizumachi-Kubono M, Inoue K , Watabe T, Ono T. Expression of GLP-1
and GLP-1 receptor in rat circumvallate papilla during growth period . The 17th International Symposium
on Olfaction and Taste 2016.06.07 Kanagawa
6. Ren E, Watari I, Hsu J, Mizumachi-Kubono M, Inoue K, Watabe T, Ono T. Unilateral nasal obstructioneﬀects taste cell in circumvallate papillae in rats. The 17th International Symposium on Olfaction and
Taste 2016.06.07 Kanagawa
7. Abe Y, Uchima Koecklin KH, Kato C, Kokai S, Ono . Eﬀects of nasal obstruction on development of the
motor representation within the facial primary motor cortex in growing rats.. The 92nd Congress of theEuropean Orthodontic Society 2016.06.11 Stockholm, Sweden
8. Kimura K, Shimazaki K, Sugimoto K, Ono T. Inﬂuence of Habitual Mouth-breathing on Taste Sensation.
The 35th Annual Meeting of the International Association for Dental Research Korean Division 2016.06.23
Seoul, Korea
9. Satokawa C, Nishiyama A, Suzuki K, Uesugi S, Kokai S, Ono T. Changes in tissue oxygen saturation
during various continuous clenching tasks. The 94th General Session & Exhibition of the International
Association for Dental Research 2016.06.24 Seoul, Korea
10. Kita S, Shimazaki K, Yajima Y, Iwai T, Omura S, Ono T. Computational ﬂuid dynamics study on
the respiratory function before/after orthognathic surgery with pyriform aperture trimming. 2016.06.24
Tokyo― 30 ―

Oral
Oral Health
Health Sciences
Sciences

11. Ohmori H, Kirimoto H, Ono T. Associations among TMDs, craniofacial morphologies, premature contact,and occlusal force in female children patients with malocclusion. The 29th Annual Meeting of the JapaneseSociety for the Temporomandibular Joint 2016.07.17 Kanagawa
12. Fukushima Y, Inoue M, Ono Take, Wake H, Nakashima T, Ono T. Reduced mastication impairs mandibular growth and memory. The 58th Annual Meeting of Japanese Association for Oral Biology 2016.08.25
Sapporo13. Ono T. Myths and facts in the Japanese Ni-Ti archwire. The 10th Asia Paciﬁc Orthodontic Conference
2016.09.01 Bali, Indonesia
14. Imamura T, Kanno Z, Wada T, Honda E, Uo M, Ono T. Inﬁltration of trace metal ions in the oral mucosa
analyzed using SR-XRF, XAFS, and ICP-MS. The 10th Asia Paciﬁc Orthodontic Conference 2016.09.01
Bali, Indonesia
15. Fukino K, Sakaguhci-Kuma T, Shimazaki K, Ono T, Akita K. An anatomical study concerning superior
region of the superior constrictor of the pharynx. The 8th international Symposium of Clinical and Applied
Anatomy 2016.09.01 Budapest, Hungry
16. Oishi S, Shimizu Y, Hosomichi J, Kuma Y,
Usumi-Fujita R, Maeda H, Nagai H, Kaneko S, Shibutani N, Suzuki J, Yoshida K, Ono T. Intermittent hypoxia inﬂuences bone microstructure via hypoxia
inducible factor and VEGF in growing rats. The 10th Asia Paciﬁc Orthodontic Conference 2016.09.01
Bali, Indonesia
17. Sakaguchi-Kuma T, Fukino K, Shimazaki K, Yamaguchi K, Okuda I, Ono T, Akita K. An anatomic
study of the functional relationships between the temporalis with reference to the anterolateral muscle
bundle and the facial muscle layer. The 8th International Symposium of Clinical and Applied Anatomy
2016.09.01 Budapest, Hungary
18. Yamaguchi H, Ishida Y, Hosoimichi J, Watanabe R, Suzuki J, Ono T. Transfection of NF-κ B decoy
into the periodontium using ultrasound-microbubble technique can suppress alveolar bone resorption inperiodontal model rats. The 59th Autum Meeting of The Japanese Society of Periodontology 2016.10.07
Niigata
19. Matsumoto Y. For the better development of occlusion: Oral myofunctional therapy and orthodontic
treatment. The 27th Annual Meeting of the Japanese Sciety of Clinical Sports Mediceine 2016.11.05
Chiba
20. Shimamine T, Yonemitsu I, Shibutani N, Koketsu M, Imai H, Fujita K, Omura S, Ono T. Changes inupper airway morphology after Le Fort I and horse-shoe osteotomies in maxillary protrusion. The 75th
Annual Meeting of the Japanese Orthodontic Society 2016.11.08 Tokushima
21. Tang H, Yonemitsu I, Ikeda Y, Miyazaki M, Shibata S, Ono T. Eﬀect of unilateral nasal obstruction onmorphology of jaw-closing muscles in growing rat. The 75th Annual Meeting of the Japanese Orthodontic
Society 2016.11.08 Tokushima
22. Imai H, Omura S, Fujita K, Honda K, Shibutani N, Yonemitsu I, Shimazaki K, Tohnai I, Ono T. Accuracyof maxillary repositioning by using SLM technique: veriﬁcation by digitalization of model surgery. The
75th Annual Meeting of the Japanese Orthodontic Society 2016.11.08 Tokushima
23. Abe Y, Kato C, Uchima Koecklin KH, Okihara H, Ishida T, Yabushita T, Kokai S, Ono T. Nasal obstruction aﬀects development of the representation within the orofacial primary motor cortex in rats. The 75th
Annual Meeting of the Japanese Orthodontic Society 2016.11.08 Tokushima
24. Hatano K, Ishida Y, Yamaguchi H, Jun Hosomichi J, Suzuki J, Kasahara Y, Oishi S, Kuma Y, Okito A,
Usumi-Fujita R, Shimizu Y, Shibutani N, Kaneko S, Ono T. The inhibition of SDF-1/CXCR4 signaling
interrupts experimental tooth movement in rats. The 75th Annual Meeting of the Japanese Orthodontic
Society 2016.11.08 Tokushima
25. Uchima Koecklin KH, Kato C, Kataguchi T, Okihara H, Ishida T, Fujita K, Yabushita T, Kokai S, Ono
T. Force changes of the tongue-protruding muscles after nasal obstruction during the growth period inrats. The 75th Annual Meeting of the Japanese Orthodontic Society 2016.11.08 Tokushima― 31 ―

Oral
HealthSciences
Oral
HealthSciences

26. Yonemitsu I, Fujita K, Imai H, Manabe A, Fukuyama E, Shibutani N, Shimazaki K, Omura S, Tohnai I,
Ono T. Retreatment of a subject with hemi-mandibular hyperplasia by orthognathic surgery and condylectomy. The 75th Annual Meeting of the Japanese Orthodontic Society 2016.11.08 Tokushima
27. Kato C, Kokai S, Ono T. An adult case of orthodontic movement with a contralateral maxillary incisor
through the midpalatal suture. The 75th Annual Meeting of the Japanese Orthodontic Society 2016.11.08
Tokushima
28. Koketsu M, Yonemitsu I, Shibutani N, Shimamine T, Imai H, Fujita K, Omura S, Ono T. Changes inupper airway morphology after Le Fort I and horse-shoe osteotomies in mandibular prognathism. The
75th Annual Meeting of the Japanese Orthodontic Society 2016.11.08 Tokushima
29. Honda K, Fujita K, Imai H, Shibutani N, Yonemitsu I, Shimazaki K, Yamashita Y, Takasu H, Murata
S, Iwai T, Hirota M, Omura S, Tohnai I, Ono T. Orthognathic surgery and implant-supported bridge ina jaw-deformity patient caused by traﬃc accidental injury. The 75th Annual Meeting of the Japanese
Orthodontic Society 2016.11.08 Tokushima
30. Watakabe K, Yonemitsu I, Ikeda Y, Miyazaki M, Ono T. Decrease in blood oxygen saturation associatedwith nasal obstruction inﬂuences mandibular morphology in growing rats. The 75th Annual Meeting of
the Japanese Orthodontic Society 2016.11.08 Tokushima
31. Kuma Y, Usumi-Fujita R, Hosomichi J, Oishi S, Yamaguchi H, Okito A, Shimizu Y, Shibutani N, Ishida
Y, Kaneko S, Suzuki J, Yoshida K, Ono T. Intermittent hypoxia alters the growth of rat nasal cavity
via the inﬂammatory pathway activation. The 75th Annual Meeting of the Japanese Orthodontic Society
2016.11.08 Tokushima
32. Shimizu Y, Aoki K, Kato G, Ono T. Occlusal disharmony-induced stress causes osteopenia of the lumbar vertebrae and long bones in mice. The 75th Annual Meeting of the Japanese Orthodontic Society
2016.11.08 Tokushima
33. Kasahara Y, Usumi-Fujita R, Kuma Y, Oishi S, Okito A, Shimizu Y, Shibutani N, Ishida Y, Kaneko S,
Hosomichi J, Ono T. Low-intensity pulsed ultrasound recovers the periodontal tissues in occlusal hypofunctional teeth. The 75th Annual Meeting of the Japanese Orthodontic Society 2016.11.08 Tokushima
34. Sakaguchi-Kuma T, Ishida Y, Kawabe A, Imamura T, Ikeda Y, Oishi S, Matsumura T, Ono T. Three
dimensional analyses of relationship between skeletal pattern and shape of mandibular alveolar bone. The
75th Annual Meeting of the Japanese Orthodontic Society 2016.11.08 Tokushima
35. Kanaguchi Arita A, Yonemitsu I, Ikeda Y, Miyazaki M, Ono T. Eﬀects of low-intensity pulsed ultrasoundstimulation on the mandibular condyle for osteoarthritis lesions. The 75th Annual Meeting of the Japanese
Orthodontic Society 2016.11.09 Tokushima
36. Usumi-Fujita R, Nakakuki K, Fujita K, Yonemitsu I, Fukuyama E, Ono T. A case with condylar hyperplasic facial asymmetry treated by unilateral alveolar segmental osteotomy. The 75th Annual Meeting of
the Japanese Orthodontic Society 2016.11.09 Tokushima
37. Uchima Koecklin KH, Hiranuma M, Kato C, Funaki Y, Kataguchi T, Yabushita T, Kokai S, Ono T.
Unilateral nasal obstruction during later growth periods aﬀects craniofacial function in rats. The 81th
Annual Meeting of The Stomatological Society, Japan 2016.11.26 Tokyo
38. Ono T. Myths and facts in Japanese Ni-Ti archwire. The 29th TAO Annual Meeting 2016.12.02 Taipei,
Taiwan39. Lai WJ, Midorikawa Y, Kanno Z, Ono T. Development of a device for three-dimensional measurement oforthodontic force systems requiring high operability.. The 29th TAO Annual Meeting 2016.12.02 Taipei,
Taiwan[Awards & Honors]
1. Yamaguchi H. The 75th Annual Meeting of the Japanese Orthodontic Society, Excellent Exhibition Award,
Japanese Orthodontic Society, 2016.11
2. Kato C. The 75th Annual Meeting of the Japanese Orthodontic Society, Excellent Exhibition Award,
Japanese Orthodontic Society, 2016.11― 32 ―

Oral
Oral Health
Health Sciences
Sciences

3. Usumi-Fujita R. The 75th Annual Meeting of the Japanese Orthodontic Society, Excellent Exhibition
Award, Japanese Orthodontic Society, 2016.11
4. Fukushima Y. The 75th Annual Meeting of the Japanese Orthodontic Society, Excellent Exhibition Award,
Japanese Orthodontic Society, 2016.11― 33 ―

Oral Health Sciences

Oral Health Sciences

Cariology and Operative Dentistry

Professor: Junji Tagami
Associate Professor: Masayuki Otsuki
Junior Associate Professor: Toru Nikaido, Masatoshi Nakajima
Assistant Professor: Takako Yoshikawa, Yasushi Shimada, Yuichi Kitasako（〜 March）, Go Inoue,
Keiichi Hosaka, Tomohiro Takagaki, Rena Takahashi
Hospital Staﬀ: Masahiro Takahashi（〜 March）, Oto Aramaki,
Naoko Matsui, Ikumi Wada（〜 March）, Yukiko Tanno（〜 March）, Ayaka Chiba（April 〜）,
Takaaki Sato（April 〜）, Kento Sato（April 〜）Specially Appointed Assistant Professor: Noriko Hiraishi, NHM Khairul Matin
JSPS Research Fellowship Noriko Hiraishi(January 〜)
Secretary: Shiori Ogi, Takako Nakagawa
Graduate Student: Shigeyuki Nagai（〜 March）, Rena Oguro（〜 March）, Takahide Ibusuki（〜 March）,
Asami Aida（〜 March）, Ayaka Chiba（〜 March）, Kei Horie（〜 March）, Tomoka Ueno（〜 March）,
Hiroki Tezuka（〜 March）, Kento Sato（〜 March）,
Takaaki Sato（〜 March）, Masami Arai（〜 March）,
Maria Nakamura（〜 March）, Ritsuko Mashiko（〜 March）,
Alaa Turkistani（〜 March）, Kong Kalyan（〜 March）,
Baba Bista（〜 March）,
Maria Jacinta Rosario Hernandez Romero（〜 March）,
Zaher Bukhari（〜 March）, Patrycja Zakilna Majkut（〜 March）,
Rui Guan（〜 September）,Ayaka Sto, Yuka Tsuda,
Nami Takashino, Takashi Hatayama,
Chihiro Matsuura, Yukinori Kano, Yuuki Naruse, Juri Hayashi,
Miho Sugiura, Yuta Sumitani, Atsuko Tagami, Keiki Nakamura, Yukari Noda, Mari Okada, Keita Taguchi,
Jorge Espigares, Junji Atomura, Thwe Zin Ei,
Hamed Atrgiran Yazdi, Tomoko Tabata, Ayako Nakamoto,Narifumi Takahashi, Takuya Nakata,
Daisuke Araoka, Luong Dao Minh Nguyet, Khine Win Zan,
Khin Yupar Kyaw, Amr Abdelaziz Aly Aly SAAD,
Michelle Sunico SEGARRA, Wa Than Lin、Dhaifallah Abdullah ALQARNI,
Ali Guzan AL-GHAMDI, Sae Akehashi, Nao Takahashi,
Yuna Kanamori, Yukina Ochiai, Kurumi Ide, Yusuke Kuno,
Yuki Ito, Shigeki Uchinuma, Daiki Nagano, Yusuke Kakinuma,
Akira Nakane, RIMA ZAKZOUK、HALABI SOMAYAH ABDULRAHMAN A、
RUMMANI GHASSAN MAHMOOD S, ALQAHTANI ALI AWAD M、
ARAVETI SANDEEP KUMAR, Hosea Lalrin Muana, Sai Kham Lyann、
Aye Ko Ko, Meiken Hayashi（April 〜）, Shou Obayashi（April 〜）,
Saori Muta（April 〜）, Kazuhide Uonekura（April 〜）, Satomi Mtsunaga（April 〜）, Shin Rozan（April 〜）,
Yukiko Tanno（April 〜）, HESHAM HASSAN OSMAN MOHAMMED（April 〜）,
AHMED MOHAMED ABDELRAHMAN ABDOU（October ）,MAHMOUD MOHAMED SAYED ALSAADI, HANI BAKHEET S（October ）,ALMASABI, WALEED ABDULQADER M（October ）,WIJETUNGA CHAMARI LASINDRA（October ）, AHMED（October ）,Soe Yupaing（October ）, Swe Zin Aung（October ）,QUTAIBA Y A A ALSANDI（October ）, San San Mayphyo Aung（October ）Research Student: Shinji Ogura, Mineo Kijima,
Erick Luz Madrigal, San San May Phyo Aung（ September）,
Khine Win Zan（April September）, Qutaiba Alsandi（April September）,
Chamari Lasindra Wijetunga（April September）, Martina Vicheva（April September）,
VICHEVA MARTINA GEORGIEVA（October ）― 34 ―

Oral Health Sciences

Oral Health Sciences(1)

OutlineTMDU possesses the longest history as a national dental university in Japan. We have contributed to theprogress of science and education through presenting a number of world leading graduates in the ﬁeld of dentistry. Many of their achievements are now recognized as global standards in the ﬁeld of dental research andclinical practice.
At Cariology and Operative Dentistry, we believe that the ultimate goal of the oral health care programs is toprovide well-being of the patients. In order to achieve this goal, besides the clinical training that we oﬀer to thelicensed graduate students, high-caliber research projects are being carried out aimed at developing, enhancing
and evaluation of the materials and techniques in dentistry; particularly for adhesives, caries prevention, diagnosis and treatment, and oral health maintenance.
Cariology and Operative Dentistry is a home to the late professor Takao Fusayama, who developed the Caries
Detector for removal of the caries, and promoted Total-etch technique and other restorative techniques
using adhesive resin composite for the minimally invasive caries treatment.
Our group, consisting of members of the faculty, staﬀ and graduate students, is among the international leadersin the ongoing dental research. I would hereby like to extend an invitation to those fellows and prospect graduate students interested in perusing high-level research and gaining an insight into modern concepts to join our
diverse international team of scientists.

(2)

Research

1) Evaluation of dentin bonding systems
Adhesion of bonding materials to enamel, dentin and cementum of tooth are evaluated using methods such as
the microshear and the microtensile bond strength tests. Factors aﬀecting adhesion such as the region and caries
state of tooth substrate, light-curing irradiation, release of ﬂuoride from material, tooth preparation methods,
root canal treatment of the tooth, etc. have been investigated. We have also focused on the diﬀerence between
various adhesives system in terms of their composition, performance and bonding durability.
2) Super Enamel and Super Dentin
Using various electron microscopy techniques, we have demonstrated that resistance of enamel and dentin toacid attack could be increased in an acid-base resistant zone which was formed following the application ofsome self-etching dental adhesives. We proposed that the diﬀusion of such acidic monomers beyond the classic
hybrid layer (interfacial zone) and their ion-exchange interactions with the available hydroxyapatite could resultin formation of stable organic-inorganic complexes, and that the structures should be termed super tooth ,which includes the reinforced enamel and dentin.
3) Development of OCT for establishing its clinical application
Optical coherent tomography (OCT) is a noninvasive, cross-sectional imaging system that can visualize theinternal structures nondestructively and without exposure to X-ray or ionizing radiation. Our research has
aimed to further develop OCT and introduce a dental OCT system that can be used to diagnose dental defects
and diseases such as tooth cracking and caries.
4) Non-destructive test of adhesive restorations
We are working to establish a method for non-destructive detection of gap and secondary caries beneath composite restorations using optical coherence tomography (OCT).
5) Evaluation of polymerization behavior of light-cured resin composites
Aim to establish clinical techniques to compensate polymerization shrinkage stress of resin composite, we evaluated eﬀect of adhesives, resin composite composition, light curing methods and cavity conﬁguration factor(Cfactor) on polymerization shrinkage stress using micro-focus X-ray computed tomography (micro-CT) and 3D
visualization method.
6) Resin coating techniqueResin coating using a bonding agent and ﬂowable composite beneﬁts the adaptation of indirect restorations todentin surface which is a key interface within a restoration. We have proposed that this resin coating technique
should be technique of choice for placement of indirect restorations.
7) Research on optical properties of the dental structure
As a part of the OCT development project, we work on characterization of the basic optical properties such asattenuation coeﬃcient and refractive index of dentin and enamel, and their changes following demineralization― 35 ―

Oral Health Sciences

Oral Health Sciences

and remineralization.
8) Research on direct core build up materials
Adhesive performance to the root canal dentin by resin core build up systems has been evaluated. These materials can be used in combination with ﬁber posts.
9) Study on dental erosion
Erosive loss of enamel due to consumption of acidic beverages and some drugs has been evaluated using 3D
focus-variation microscopy as well as proﬁlometry.
10) Caries risk assessment
We have investigated caries risk based on the measurement of saliva buﬀering capacity in samples collected frompatients. We have also probed the association between the pH of lesion surface and caries activity.
11) Adhesion of cariogenic bacteria to dentin surface
We have developed a model to experimentally evaluate factors aﬀecting the ability of cariogenic bacteria suchas S.mutans to attach to the tooth surface in the initial phase of bioﬁlm formation.
12) Biocompatibility of resin-based dental adhesives
Immunohistochemical studies have been performed to evaluate the eﬀects of various adhesive materials on dental
pulp tissue.
13) The potential of ﬂuoride- and/or Calcium containing materials on caries prevention
Inhibitory eﬀects of CPP-ACP paste and ﬂuoride on the enamel and dentin demineralization have been evaluated by the micro-focus X-ray computed tomography (micro-CT) non-destructively. We have also establisheda standard methodology for assessment of lesion parameters such as depth and mineral loss for micro-CT.
14) Evaluation of caries removal methods
We have evaluated the eﬀect of caries removal method by the conventional rotary cutting instruments in comparison with new caries removal methods such as chemical removal agents, laser irradiation and abrasion on theadhesion performance and restoration success.
15) Development and evaluation of aesthetic dental materials
We have worked on optical properties and color match of the composite resins, in addition to clinical applications of tooth whitening materials .
16) Clinical research
We have created a protocol to evaluate the long-term and short-term performance of restorative materials in
the patients who were admitted to the operative dentistry clinics at TMDU Dental Hospital.

(3)

Education

Cariology and Operative Dentistry section oﬀers a four-year graduate program. First-year graduate students
attend lectures and seminars given in the graduate school and are expected to gain an understanding of thefundamentals about methodology and the knowledge necessary for their research. The contents of the classes
given in our section include topics related to cariology and operative dentistry: caries diagnosis, biocompatibility,
caries treatment and restoration, prevention and control, dental materials, new instruments and equipment. In
keeping with the internationally orientated philosophy of this section, lectures are conducted in English andare open to all foreign students. First-year graduate students also undergo clinical training the procedures ofmodern adhesive restorations. Laboratory work, which commences in the ﬁrst year, is performed under thesupervision of our faculty staﬀ. During the four-year program, several papers are required to be presented
in domestic and / or international conferences and submitted to journals. The minimum requirements arecompleting the prescribed courses, a supervised research project and a dissertation for the degree published ina top international journal.

(4)

Lectures & Courses

The ultimate goal of the oral health care programs is to provide well-being of the patients. In order to achieve
this goal, besides the clinical training that we oﬀer to the licensed graduate students, high-caliber research
projects are being carried out aimed at developing, enhancing and evaluation of the materials and techniques indentistry; particularly for adhesives, caries prevention, diagnosis and treatment, and oral health maintenance.

― 36 ―

Oral Health Sciences

Oral Health Sciences(5)

Clinical Services & Other Works

Full-time faculty see patients in Operative Dentistry and Endodontics, and provide restoration of teeth withﬁllings for dental cavities, trauma and tooth wear, and root canal treatments. The faculty members supervise
both pre-and postdoctoral students in the clinic.
(6)

Clinical Performances

Our Operative Dentistry clinic provide restoration of teeth with ﬁllings for dental cavities, trauma and tooth
wear under Minimal intervention concept. The clinical services are based on accumulated scientiﬁc researches.
(7)

Publications[Original Articles]
1. Takako Yoshikawa, Makoto Morigami, Alireza Sadr, Junji Tagami . Environmental SEM and dye penetration observation on resin-tooth interface using diﬀerent light curing method Dental Materials Journal.
2016; 35(1); 89-96
2. Chiba A, Zhou J, Nakajima M, Tan J, Tagami J, Scheﬀel DLS, Hebling J, Agee KA, Breschi L, Grégoire
G, Jang SS, Tay FR, Pashley DH. The eﬀects of ethanol on the size-exclusion characteristics of type Ⅰ
collagen to adhesive resin monomers Acta Biomaterialia. 2016;
3. Sachiko Ito, Yasushi Shimada, Alireza Sadr, Yukie Nakajima, Michiyo Miyashin, Junji Tagami, Yasunori
Sumi. Assessment of occlusal ﬁssure depth and sealant penetration using optical coherence tomography.
Dent Mater J. 2016; 35(3); 432-439
4. Hiroki Tezuka, Yasushi Shimada, Khairul Matin, Masaomi Ikeda, Alireza Sadr, Yasunori Sumi, Junji
Tagami. Assessment of cervical demineralization induced by Streptococcus mutans using swept-source
optical coherence tomography. J Med Imaging (Bellingham). 2016.01; 3(1); 014504
5. A Chiba, J Zhou, M Nakajima, J Tan, J Tagami, D L S Scheﬀel, J Hebling, K A Agee, L Breschi, G
Grégoire, S S Jang, F R Tay, D H Pashley. The eﬀects of ethanol on the size-exclusion characteristics oftype I dentin collagen to adhesive resin monomers. Acta Biomater. 2016.03; 33; 235-241
6. Maria Jacinta Rosario H Romero, Syozi Nakashima, Toru Nikaido, Alireza Sadr, Junji Tagami. In vitro
dentine remineralization with a potential salivary phosphoprotein homologue. Arch. Oral Biol.. 2016.03;
68; 35-42
7. Baba Bista, Syozi Nakashima, Toru Nikaido, Alireza Sadr, Tomohiro Takagaki, Maria J R H Romero,
Takaaki Sato, Junji Tagami. Adsorption behavior of methacryloyloxydecyl dihydrogen phosphate on an
apatite surface at neutral pH. Eur. J. Oral Sci.. 2016.04; 124(2); 195-203
8. Md Soﬁqul Islam, Sahar Jameel Khunkar, Syozi Nakashima, Alireza Sadr, Toru Nikaido, Junji Tagami.
Comparative study of demineralized collagen degradation determined by hydroxyproline assay and microscopic depth measurement. J Dent. 2016.04; 47; 94-97
9. Takaaki Sato, Tomohiro Takagaki, Naoko Matsui, Hidenori Hamba, Alireza Sadr, Toru Nikaido, Junji
Tagami. Morphological Evaluation of the Adhesive/Enamel interfaces of Two-step Self-etching Adhesives
and Multimode One-bottle Self-etching Adhesives. J Adhes Dent. 2016.06; 18(3); 223-229
10. Rui Guan, Tomohiro Takagaki, Naoko Matsui, Takaaki Sato, Michael F Burrow, Joseph Palamara, Toru
Nikaido, Junji Tagami. Dentin bonding performance using Weibull statistics and evaluation of acid-baseresistant zone formation of recently introduced adhesives. Dent Mater J. 2016.06;
11. Junichi Shinagawa, Go Inoue, Toru Nikaido, Masaomi Ikeda, Alireza Sadr, Junji Tagami. Dentin bonding
performance and interface observation of an MMA-based restorative material. Dent Mater J. 2016.06;
12. Ornnicha Thanatvarakorn, Taweesak Prasansuttiporn, Masahiro Takahashi, Suppason Thittaweerat, Richard
M Foxton, Shizuko Ichinose, Junji Tagami, Masatoshi Nakajima. Eﬀect of Scrubbing Technique with Mild
Self-etching Adhesives on Dentin Bond Strengths and Nanoleakage Expression. J Adhes Dent. 2016.06;
18(3); 197-204― 37 ―

Oral Health Sciences

Oral Health Sciences

13. Wataru Komada, Tasuku Inagaki, Yoji Ueda, Satoshi Omori, Keiichi Hosaka, Junji Tagami, Hiroyuki
Miura. Inﬂuence of water immersion on the mechanical properties of ﬁber posts. J Prosthodont Res.
2016.06;
14. Kanae Wada, Eri Ikeda, Junichiro Wada, Go Inoue, Munenaga Miyasaka, Michiyo Miyashin. Wearcharacteristics of trimethylolpropane trimethacrylate ﬁller-containing resins for the full crown restorationof primary molars. Dent Mater J. 2016.06; 35(4); 585-593
15. Ehab Z Alsayed, Ilnaz Hariri, Syozi Nakashima, Yasushi Shimada, Turki A Bakhsh, Junji Tagami, Alireza
Sadr. Eﬀects of coating materials on nanoindentation hardness of enamel and adjacent areas. Dent Mater.
2016.06; 32(6); 807-816
16. Takaaki Sato, Tomohiro Takagaki, Naoko Matsui, Hidenori Hamba, Alireza Sadr, Toru Nikaido, Junji
Tagami. Morphological Evaluation of the Adhesive/Enamel interfaces of Two-step Self-etching Adhesives
and Multimode One-bottle Self-etching Adhesives. J Adhes Dent. 2016.06; 18(3); 223-229
17. Minh Nguyet Dao Luong, Yasushi Shimada, Alaa Turkistani, Junji Tagami, Yasunori Sumi, Alireza Sadr.
Fractography of interface after microtensile bond strength test using swept-source optical coherence tomography. Dent Mater. 2016.07; 32(7); 862-869
18. Tomoka Ueno, Yasushi Shimada, Khairul Matin, Yuan Zhou, Ikumi Wada, Alireza Sadr, Yasunori Sumi,
Junji Tagami. Optical analysis of enamel and dentin caries in relation to mineral density using sweptsource optical coherence tomography. J Med Imaging (Bellingham). 2016.07; 3(3); 035507
19. Rena Oguro, Masatoshi Nakajima, Naoko Seki, Alireza Sadr, Junji Tagami, Yasunori Sumi. The role ofenamel thickness and refractive index on human tooth colour. J Dent. 2016.08; 51; 36-44
20. Asami Aida, Masatoshi Nakajima, Naoko Seki, Yukinori Kano, Richard M Foxton, Junji Tagami. Eﬀect
of enamel margin conﬁguration on color change of resin composite restoration. Dent Mater J. 2016.08;
35(4); 675-683
21. Ornnicha Thanatvarakorn, Md Soﬁqul Islam, Syozi Nakashima, Alireza Sadr, Toru Nikaido, Junji Tagami.
Eﬀects of zinc ﬂuoride on inhibiting dentin demineralization and collagen degradation in vitro: A comparison of various topical ﬂuoride agents. Dent Mater J. 2016.08;
22. Yuan Zhou, Yasushi Shimada, Khairul Matin, Alireza Sadr, Yasunori Sumi, Junji Tagami. Assessment ofbacterial demineralization around composite restorations using swept-source optical coherence tomography(SS-OCT). Dent Mater. 2016.09; 32(9); 1177-1188
23. Kei Horie, Yasushi Shimada, Khairul Matin, Masaomi Ikeda, Alireza Sadr, Yasunori Sumi, Junji Tagami.
Monitoring of cariogenic demineralization at the enamel-composite interface using swept-source opticalcoherence tomography. Dent Mater. 2016.09; 32(9); 1103-1112
24. Kalyan Kong, Noriko Hiraishi, Mohannad Nassar, Masayuki Otsuki, Cynthia K Y Yiu, Junji Tagami. Effect of phytic acid etchant on resin-dentin bonding: Monomer penetration and stability of dentin collagen.
J Prosthodont Res. 2016.10;
25. Takako YOSHIKAWA, Alireza SADR, Makoto ARAKAWA, Junji TAGAMI. . Bond strength of resin
composites to cavity ﬂoor and cavity wall dentin. Asian Paciﬁc Journal of Dentistry. 2016.11; 16; 23-26
26. Miho Sugiura, Yuichi Kitasako, Alireza Sadr, Yasushi Shimada, Yasunori Sumi, Junji Tagami. White
spot lesion remineralization by sugar-free chewing gum containing bio-available calcium and ﬂuoride: A
double-blind randomized controlled trial. Journal of Dentistry. 2016.11; 54; 86-91
27. Takako YOSHIKAWA, Alireza SADR, Junji TAGAMI. . Eﬀects of C-factor on bond strength to ﬂoor
and wall dentin. Dental Materials Journal. 2016.12; 35(6); 918-922
28. Iori Sugita, Syozi Nakashima, Masaomi Ikeda, Michael F Burrow, Toru Nikaido, Shisei Kubo, Junji
Tagami, Yasunori Sumi. A pilot study to assess the morphology and progression of non-carious cervicallesions. J Dent. 2016.12;― 38 ―

Oral Health Sciences

Oral Health Sciences[Books etc]
1. Junji Tagami. Quarterly dental care. First publication, 2016.04

2. ryo imai,ritu aoki,reiko kaduki,hiko hyakusoku. rehabilitation makeup-To be visible makeup therapy
option-. kokuseidou publishing company, 2016.06
3. nico. Quintessence publishing, 2016.08[Misc]
1. 3. Yasuko Momoi, Akihiko Shimizu, Mikako Hayashi, Satoshi Imazato, Masako Unemori, Yuichi Kitasako,
Shisei Kubo, Rena Takahashi, Shozi Nakashima, Toru Nikaido, Masayoshi Fukushima, Morioki Fujitani,
Chinami Yamaki, Kenichi Sugai. Root caries management: Evidence and consensus based report Curr
Oral Health Rep. 2016.03; 30; 1-7[Conference Activities & Talks]
1. Keiichi Hosaka. Direct Composite Restorations. Invited Lecture 2016.01.15 Trisakty University, Jakarta,
Indonesia
2. Keiichi Hosaka. Lecture Direct Veneer & Class 3-4 Restorations. Sminar Sehari Tentang Estetik Veneer,
Hukum, Kode Etik Dan Perpajakan 2016.01.16 Jakarta, Indonesia
3. Keiichi Hosaka. Hands On Direct Veneer & Class 3-4 Restorations. Sminar Sehari Tentang Estetik Veneer,
Hukum, Kode Etik Dan Perpajakan 2016.01.16 Jakarta, Indonesia
4. Yasushi Shimada. Optical coherence tomography as a new tool for caries diagnosis and assessment of
restoration. KPPIKG 2016 2016.02.27 Jakarta
5. Yuan Zhou, Khairul Matin. Evaluation of Resin Inﬁltration to Prevent Progression of Root Caries.
American Association for Dental Research (AADR) Annual Meeting 2016.03.16 Los Angeles, Calif, USA
6. Yuki Naruse, Tomohiro Tkakagaki, Naoko Matsui, Takaaki Sato, Toru Nikaido, Masaomi Ikeda, Junji
Tagami. Eﬀects of alumina-blasting for a CAD/CAM resin block on adhesion. 45th Annual meeting ofAmerican Association for Dental Research 2016.03.17 Los Angels
7. Tomoko TABATA, Yasushi SHIMADA, Alireza SADR, Junji TAGAMI, Yasunori SUMI. Assessment ofEnamel Crack at Cavosurface Margin Using 3D SS-OCT. 45th annual meeting of AADR 2016.03.17 Los
Angeles
8. Chihiro MATSUURA, Yasushi SHIMADA, Alireza SADR, Junji TAGAMI, Yasunori SUMI . 3D assessment of dentin caries under composite restorations using SS-OCT . AADR 2016.03.17 Los Angeles
9. Rui Guan, Tomohiro Takagaki, Toru Nikaido, Takahiro Wada, Motohiro Uo, Junji Tagami. Adsorption
behavior of phosphoric functional monomers to 3Y-TZP surface.. 45th Annual meeting of American
Association for Dental Research 2016.03.17
10. Ornnicha Thanatvarakorn, Taweesak Prasansuttiporn, Masahiro Takahashi, Suppason Thittaweerat, Junji
Tagami, Masatoshi Nakajima. Scrubbing eﬀect of one-step self-etch adhesives on dentin bonding. 45th
AADR 2016.03.18 Los Angeles, Calif
11. Masatoshi Nakajima, Ornnicha Thanatvarakorn, Taweesak Prasansuttiporn, Keiichi Hosaka, Junji Tagami.
Eﬀect of smear layer-deproteinizing on dentin bonding of self-etch adhesives. 45th AADR 2016.03.18 Los
Angeles, Calif
12. Taweesak Prasansuttiporn, Ornnicha Thanatvarakorn, Junji Tagami, Masatoshi Nakajima. Bonding durability of dental adhesive to smear layer-deproteinized dentin. 45th AADR 2016.03.18 Los Angeles, Calif
13. David Pashley, Ayaka Chiba, Jianfeng Zou, Masatoshi Nakajima, Jianguo Tan, Junji Tagami, Debora
Scheﬀel, Josimeri Heblingm, Kelli Agee, Lorenzo Breschi, Genevieve Gregorie, Seung Jang, Franklin Tay.
Eﬀect of ethanol on size-exclusion characteristics of dentin-collagen to monomer. 45th AADR 2016.03.18
Los Angeles, Calif.

― 39 ―

Oral Health Sciences

Oral Health Sciences

14. Inoue G, Mashiko R, Nakashima S, Tagami J. Surface assessment on artiﬁcial caries-aﬀected dentin with
FCP-COMPLEX. 45th Annual Meeting of American Association for Dental Research 2016.03.19 Los
Angeles, USA
15. Prof. Nairn H F Wilson. History and reasons behind current trends in dentistry in the UK. 2016.04.04
Tokyo medical and dental university
16. Junji Tagami. About composite resin restration. 2016.05.18
17. Noriko Hiraishi, Fumiaki Hayashi, Tomohiro Takagaki, Masayuki Otsuki, Junji Tagami. Interaction of
MDP-based adhesive with dentin studied by solid-state NMR. 2016.06 Seoul, Korea
18. Microtensile bond strength to dentin of a newly developed one-step self-etch system containing hydrophilic
amide monomer. 2016.06.09
19. Sae AKEHASHI, Rena TAKAHASHI, Toru NIKAIDO, Junji TAGAMI. Enhancement of Dentin Bond
Strength of Resin Cement using New Resin Coating Materials. The 144th Meeting of the Japanese Societyof Conservative Dentistry 2016.06.10 Tochigi, Japan
20. Khin Yupar Kyaw, Masayuki Otsuki, Junji Tagami . Eﬀect of application of sodium ﬂuoride on in-oﬃce
bleaching. 2016.06.10
21. Nagano Daiki. The eﬀect of surface treatment on repairing procedure of aged resin composite in thediﬀerent term of water immersion. 2016.06.10
22. Rainer Guggenburger. How innovation comes to live in dentistry - from idea to clinical success. Special
lecture 2016.06.17 Tokyo medical and dental University Cariology and Operative Dentistry
23. Aramaki O, Kawashima N, Shimada Y, Okiji T, Tagami J . Three-dimensional analysis of Iba1+ macrophages
in human dental pulp using whole mount immunostaining. 94th General Session & Exhibition of the IADR
2016.06.22 Seoul, Korea
24. Yasushi Shimada, Juri Hayashi, Ikumi Wada, Oto Aramaki, Alireza Sadr, Yasunori Sumi, Junji Tagami.
3D Assessment of Dental Caries Using Swept-source Optical Coherence Tomography in Vivo. 94th Generalsession & exhibition of the IADR 2016.06.23 Seoul, Korea
25. Rena Takahashi, Yasushi Shimada, Yu-Chih Chiang, Ikumi Wada, Yasunori Sumi, Junji Tagami. Comparison of light-cured pit and ﬁssure sealants on enamel demineralization using swept-source optical coherence
tomography. 94th General Session & Exhibition of the IADR 2016.06.24 Seoul, Korea
26. Yu-Jung Lai, Ting-Chen Liu, Szu-Ying Huang, Rena Takahashi, Yasushi Shimada, Junji Tagami, Yu-Chih
Chiang. Eﬀects of S-PRG Fillers-containing Composite on Demineralization Inhibition of Enamel Surface:
Optical Coherence Tomography (OCT) and Micro Computed Tomography (Micro CT) Approaches. 94th
General Session & Exhibition of the IADR 2016.06.24 Seoul, Korea
27. Junji ATOMURA. Inﬂuence of FCP-COMPLEX on bond strength to caries-aﬀected dentin. 2016 IADR/APR
General Session in seoul 2016.06.24 KOREA
28. Otsuki M, Sawada K, Arakawa S, ShibaA, ShiodaG, Tagami J. Eﬀect of ozone gel on tooth bleaching.
94th General Session of the IADR 2016.06.25 Seoul, Korea
29. Otsuki M, Nagai S, Alireza S, Shimada Y, Hayashi J, Tagami J, Sumi Y. Eﬀect of Er:YAG laser preparation
on resin-cavity interface evaluated by SS-OCT. 2016 International Conference od QLF 2016.06.26 Seoul,
Korea
30. Noda S, Kawashima N, Hashimoto K, Aramaki O, Yamamoto M, Okiji T. Eﬀects of conﬂuent culture
condition on dental pulp stem cell surface markers, proliferation, gene expression. IADR Pulp Biology
and Regeneration Group Satellite Symposium 2016 2016.06.28 Nagoya
31. Akira Nakane, Masayuki Otsuki, Junji Tagami . Change of dentin surface by low-power irradiation of
Er:YAG laser. 15th Congress of the World Federation for Laser Dentistry 2016.07.19 Nagoya
32. Yuki Ito,Masayuki Otsuki, Junji Tagami, Takao Asaki, Norika Okawara. Eﬀect of pH conditioners on
tooth bleaching. 2016.08.27― 40 ―

Oral Health Sciences

Oral Health Sciences

33. Eﬀect of cavity depth on Ultimate Tensile Strength of bulk ﬁll ﬂowable resin. 2016.09.10
34. Yuan Zhou, Yasushi Shimada, Khairul Matin, Yasunori Sumi, Junji Tagami. . Assessment of Root Caries
under Wet and Dry Conditions Using Swept-source Optical Coherence Tomography (SS-OCT).. The
145th Meeting of the Japanese Society of Conservative Dentistry (JSCD). 2016.10 Nagano, Japan.
35. Keiichi Hosaka. Adhesive Dentistry meets Direct Composite Restoration. Invited Lecture 2016.10.14
Chulalongkorn University, Bangkok, Thailand
36. Junji Tagami. Creating a future dentistry with the adhesive dentistry. Research Day with CU,UI,UMPandTMDU
Dental Students 2016 2016.10.18 Akio Suzuki memorial Hall in TMDU
37. Yuna KANAMORI, Rena TAKAHASHI, Toru NIKAIDO, Junji TAGAMI. The eﬀect of light irradiation on dentin bond strengths of dual-cure resin cements. The 145th Meeting of Japanese Society ofConservative Dentistry 2016.10.27 Nagano, Japan
38. Yukina Ochiai, Go Inoue, Junji Atomura, Toru Nikaido, Junji Tagami. The evaluation of bond strengthof calcium containing adhesive system . 2016.10.27 Nagano
39. Junji Tagami. The top researcher talks "The best adhesion". 2016.11.13 Tokyo international forum
40. Junji Tagami. New trend of dental materials, adehesive resin and composite resin. 2016.11.23 Study
medicine in Czech Republic
41. Zhou Yuan, Matin Khairul, Shimada Yasushi, Sumi Yasunori, Tagami Junji. . Evaluation of ResinInﬁltration to Reduce Enamel Cracks by Swept-source Optical Coherence Tomography (SS-OCT).. The
35th Annual Meeting of Japan Society for Adhesive Dentistry 2016.12 Hokkaido, Japan
42. Sato Takaaki, Takagaki Tomohiro, Nikaido Toru, Tagami Junji. Observation of Acid-base Resistant Zone
on adhesive/enamel interface using newly developed one step self-etching adhesive. The 35th Annual
Meeting of Japan Society for Adhesive Dentistry 2016.12.03
43. Interfacial adaptation of composite restorations before and after light-curing .
44. Toothbrushing timing after acidic drinks aﬀects enamel loss in-situ. Boston, USA
45. The evaluation of bond strength of calcium containing adhesive system to artiﬁcial demineralized dentin.

― 41 ―

Oral
HealthSciences
Oral
HealthSciences

Fixed Prosthodontics
Professor
Hiroyuki MIURA
Associate Professor
Kenichi YOSHIDA
Junior Associate Professor
Daizo OKADA
Wataru KOMADA
Assistant Professor
Chiharu SHIN
Shiho OTAKE
Reiko OGURA
Satoshi OMORI
Reina NEMOTO
Attending Staﬀ
Tasuku INAGAKI
Yoji UEDA
Rie FUJITA
Tazuko MAKIYAMA
Hiroyuki OKAMOTO
Miho SATO
Kyoshi MATSUKAWA
Fujino OSHIMA
Yoko ISHIKAWA
Natsuko IWATA
Mariko KUBO
Kazuhisa FUJITA
Hideto MATSUI
Risa YAMADALuo Siyang
Michika MINAMIFUCHI
Ayana URABA
Bakhit Mohammed Yassin M
Paisankobrit Vibul
Rana ASANO
Ayaka SHIRASAKI
Mina TAKITA
Shiro RIKITOKUKai SHIBAGUCHI
Kiriko SUGANO
Kenichiro HAYASHI
Mayuko MATSUMURA(1)

Research

1)Occlusion and Mastication.(mandibular position, mandibular movement, articulator, masticatory eﬃciency)
2)Inﬂuence of mechanical stress caused by occlusal contact on stomatognathic system. (Tooth displacement,
distortion of alveolar bone, occlusal contact, proximal contact etc.)― 42 ―

Oral
Oral Health
Health Sciences
Sciences

3)Relationship of main occluding area and occlusal contact4)Research on post and core(materials, stress analysis etc.)
5)Clinical application of latest technology and development of new materials (CAD/CAM, Zirconia, opticalimpression etc.)
6)Inﬂuence of occlusal contact for an important prosthesis on the periodontal tissues of the antagonist.
7)Application of laser welding in crown and bridge restorations.
8)Inﬂuence of dental materials for periodontal tissues and biological body.
9)Functional analysis of abnormal stomatognathic function(2)

Lectures & Courses

The major subjects of the studies are occlusion of Cr-Br prostheses (ﬁxed restoration such as crown and ﬁxed
partial denture), analysis of mandibular movement, inﬂuence of crown and periodontal tissue and its systemicaﬀect, accuracy of manufacturing processes of crown (i.e. casting, soldering, luting and adjustment of occlusion), functional analysis of stomatognathic system and development of apatite ceramic implant. The research
themes are investigated with measurement systems of mandibular movement, measuring instruments of tooth
micro-displacement, electromyography, measurement apparatus of dimensional accuracy, EPMA (electron probe
microanalyzer) for analyzing very small amount of dental alloy and histopathological methods. Clinical training
and general lecture on prosthodontics are prepared for the graduate students in the ﬁrst year. After the second
year they will have special training for their research methods and experiments will be performed according
to the research plan. In the last year the students will write the paper for thesis under the direction of theprofessor.

(3)

Clinical Services & Other Works

1) Clinic for prosthodontics (Prosthodontics practice clinic)
This clinic is organized by clinical teams, and 4 to 8 dentists compose 1 team working in cooperation between
teams. Here oﬀers a complete range of restorative, rehabilitative, and esthetic dentistry, treatment types include
since simple one teeth to complete oral rehabilitation using the latest technologies.
2) Clinic for dental allergy (Dental allergy clinic)
This clinic provides allergy tests test for dental alloys and dental materials on potential patients before dental
treatment, besides, patients with skin and/or oral deseases histories induced by previous dental restorations.
The causal allergen/s is/are identiﬁed by patch tests or if some metal restoration is allergy set on, is analyze
by Electron Probe Micro Analyzer (EPMA), removing out only restoration such content allergens.

(4)

Publications[Original Articles]
1. Oshima Ｆ, Okada Ｄ, Ogura R, Shin C, Ueda Y, Inagaki T, Fujita R, Miura H. A ﬁnite element analysisof stress distribution in roots with diﬀerent types of post systems. Asian Paciﬁc Journal of Dentistry.
2016; 16(1); 1-7
2. Kosuke Nozaki, Risa Yamada, Hiroyuki Miura, Kimihiro Yamashita, Akiko Nagai. Antibacterial activityof silver nanoparticles coanting on zirconia against oral bacteria Visual-JW 2016. 2016; 41― 43 ―

Oral
HealthSciences
Oral
HealthSciences

3. Ana Milheiro, Kosuke Nozaki, Cornelis J Kleverlaan, Joris Muris, Hiroyuki Miura, Albert J Feilzer. In
vitro cytotoxicity of metallic ions released from dental alloys. Odontology. 2016.05; 104(2); 136-142[Conference Activities & Talks]
1. Omori S, Takeuchi K, Inagaki T, Miura H. Relationship between framework thickness and fracture strengthof Ce-TZP/Al crown. 45th Annual Meeting & Exhibition of the AADR 2016.03.16 Los Angels, California
2. Ishikawa Y, Komada W, Inagaki T, Nemoto R, Omori S, and Miura H. Root Surface-strain of Bridge
Using Diﬀerent Post and Core Systems. AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los
Angeles, CA, USA
3. Fujita R, Takita M, Otake S, Shirasaki A, Miura H. Bond strengths of cements to a new indirect resin
composite. AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los Angeles, CA, USA
4. Iwata N, Nozaki K, Miura H, Yamashita K, Nagai A. Surface modiﬁcation of Ti implant with controlled
micro/nano-structured topography. AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los Angeles,
CA, USA
5. Risa Yamada, Kosuke Nozaki, Hiroyuki Miura, Kimihiro Yamashita and Akiko Nagai. Antimicrobial activity of silver nanoparticle-coated yttria-stabilized zirconia. AADR/CADR Annual Meeting & Exhibition
2016.03.17 Los Angeles, CA, USA
6. Minamifuchi.M, Nemoto.R, Inagaki.T, Uraba.A, Nozaki.K and Miura.H. Inﬂuence of framework design
on shear stress distribution . AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los Angeles, CA,USA
7. Nemoto R, Shirasaki A , Otake S , Takita M, Miura H. Bond strengths of cements to a new CAD/CAM
resin composite . AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los Angeles, CA, USA
8. Otake S, Uraba A, Minamifuchi M, Miura H. Bond strengths of cements to a new polyetheretherketone
material. AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los Angeles, CA, USA
9. Uraba A, Inagaki T, Nemoto R, Minamifuchi M, Nozaki K, Miura H. The shear stress distribution of two
diﬀerent cantilever RBFPDs. AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los Angeles, CA,
USA
10. Kubo M, Komada W, Otake S, Inagaki T, Omori S, Miura H. Fracture strength of composite resin corewith glass ﬁber ribbon.. AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los Angeles, CA, USA
11. Asano R, Okada D, Shin C, Rikitoku S, Shirasaki A, Takita M, Shibaguchi K, Uraba A, Minamifuchi M,
Ishikawa Y, Iwata N, Kubo M, Fujita K, Matsui H, Yamada R, Matsukawa K and Miura H. Statistical
Analysis of Prosthetic Restorations in year 2014-2015 at Tokyo Medical and Dental University ― Single
Restorations— . AADR/CADR Annual Meeting & Exhibition 2016.03.18 Los Angeles, CA, USA
12. Rikitoku S, Omori S, Okada D, Shin C, Asano R, Shirasaki A, Takita M, Shibaguchi K, Uraba A,
Minamifuchi M, Ishikawa Y, Iwata N, Kubo M, Fujita K, Matsui H, Yamada R, Matsukawa K and Miura H.
Statistical Analysis of Prosthetic Restorations in year 2014-2015 at Tokyo Medical and Dental University- Dental Prosthesis for Missing Teeth - . AADR/CADR Annual Meeting & Exhibition 2016.03.18 Los
Angeles, CA, USA
13. Shibaguchi K, Okamoto H, Noda A, Matsui H, Fukumoto I, Hani H, Kitazaki H, Matsumura M, Miura
H. Recent trends in Relationship between Pustulosis Palmoplantaris and Metal Allergy. 2016.03.19
AADR/CADR Annual Meeting & Exhibition Los Angeles, CA, USA
14. Fujita K, Nozaki K, Yamashita K, Miura H, and Nagai A. Diﬀerentiation of periodontal ligament cell onhydroxyl- and carbonated-apatites. AADR/CADR Annual Meeting & Exhibition 2016.03.19 Los Angeles,
CA, USA
15. Nozaki K, Fujita K, Miura H, Yamashita K, Nagai A. Bone remodeling-optimization of carbonated apatite
for guided bone regeneration. AADR/CADR Annual Meeting & Exhibition 2016.03.19 Los Angeles, CA,USA― 44 ―

Oral
Oral Health
Health Sciences
Sciences

16. Kosuke Nozaki, Kazuhisa Fujita, Naohiro Horiuchi, Miho Nakamura, Hiroyuki Miura, Kimihiro Yamashita, Akiko Nagai. In vivo evaluation of the role of carbonate ion in hydroxyapatite for bone remodeling. 10th World Biomaterials Congress 2016.05.20
17. Matukawa K,Nemoto R, Inagaki T, Kubo M, Oomori T, Yoshida K, Miura H. Tensile Bond Strengths ofResin Cements to Zirconia Fixed Partial Dentures. 2016.05.28 Sapporo, Japan
18. W. Komada, S. Otake, R. Nemoto, K. Yoshida, H. Miura . Pull-out strength of glass ﬁber post withcomposite resin. The 27th Annual Meeting of Japan Academy of Esthetic Dentistry 2016.08.27 Sapporo
19. Kosuke Nozaki, Risa Yamada, Hiroyuki Miura, Kimihiro Yamashita, Akiko Nagai. Antibacterial activityof silver nanoparticles coating on zirconia against oral bacteria. The 1st International Symposium on
Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development
2016.10.17― 45 ―

Oral Health Sciences

Oral Health Sciences

Pulp Biology and Endodontics

Professor:
Takashi OKIJI
Associate Professor:
Mitsuhiro SUNAKAWA
Junior Associate Professor:
Atsushi TAKEDA, Hideharu IKEDA
Assistant Professor:
Arata EBIHARA, Nobuyuki KAWASHIMA, Hiroyuki MATSUMOTO, Reiko WADACHI,
Noriyuki SUZUKI, Tomoatsu KANEKO, Satoshi WATANABE, Jun KAWAMURA
Hospital Staﬀ:
Kei KOMATSU, Mioko YAMAMOTO, Yasumiko WAKABAYASHI, Yoshiko IINO,
Shintaro URABA, Kazuhisa SATAKE, Hiroyuki HARADA
Graduate Student:
Kazuto FURUHATA, Sailiman AIERKEN, Kento TAZAWA, Daisuke TOKITA, Kanako YAO,
Alamuddin BAKHIT, Miki NISHIJO, Kenrato HASHIMOTO, Donghoon KANG, Keisuke NARA,
Sonoko NODA, Tomoyuki HONGO, Mayuko FUJII, Bayan RASHED, Youhei FUKUMORI,
Yuki KASAHARA, Shunsuke KIMURA, Masashi KURAMOTO, Bin GU,
Akira KOUNO, Yasuhiro HOSHIHARA, Keiichiro MAKI, Shinya YAMAUCHI,
Thaw Dar OO, Phyo Pyai SONE, Su Yee Myo ZAW, Pyae Hein HTUN
Research Student:
Shota IKEDA, Shingo AOKI, Masahiko KUSANO, Natumi SABA,
Yadanar Su PHYO, Xiang AO(1)

OutlineThe Department of Pulp Biology and Endodontics deals with endodontics/endodontology, and is concernedwith research and clinical practice on the prevention, diagnosis and treatment of dental pulp and periapicaldiseases. In order to preserve and well maintain the function of the teeth in the oral cavity, it is importantto understand the structural and functional features of the dental pulp and protect it carefully from noxious
stimuli. However, pulp diseases, if left untreated, may progress to develop pulp necrosis and apical periodontitis,where meticulous treatment is required to eliminate infection from the complex root canal system. The goal ofendodontics is to achieve long term maintenance of tooth function by the prevention and treatment of pulpal
and periapical diseases.

― 46 ―

Oral Health Sciences

Oral Health Sciences(2)

Research

1) Dental pulp tissue engineering
2) Cell biology of odontoblasts, pulp calls and dental pulp stem cells
3) Analysis of regulatory factors associated with pulp inﬂammation
4) Root canal irrigation regimens for pulp regeneration therapy5) Laser-activated root canal irrigation
6) Bioactivity evaluation of newly developed pulp capping materials and root canal sealers
7) Mechanical property analysis of NiTi rotary instruments for safe and eﬃcient root canal preparation
8) Evaluation of cone-beam computed tomography in the diagnosis of endodontic lesions9) Application of optical coherence tomography to endodontic diagnosis(3)

Education

The educational aim of the Department of Pulp Biology and Endodontics is to cultivate students so that they
can obtain knowledge and skills required for leading scientists, researchers or practitioners of endodontics. Since
recent progress of pulp biology and endodontics is remarkable, the students are educated to acquire the newest
knowledge on modern endodontology and its related subjects, such as neuroscience, microbiology, molecularbiology, immunology and biomaterial sciences, and are trained to master the newest technology of endodontics.
All the students are asked to add new ﬁndings to the ﬁeld of endodontics based on their own original research.
(4)

Clinical Services & Other Works

The Department of Pulp Biology and Endodontics is in charge of the Clinic of Operative Dentistry and Edodontics in our Dental Hospital, together with the Department of Cariology & Operative Dentistry, and oﬀers theglobal standard of care in the treatment of pulpal and periapical diseases to our patients. We provide clinicalcare in the full spectrum of endodontics including;· Vital pulp therapies,· Nonsurgical root canal therapies,· Root canal retreatments,· Endodontic microsurgeries,· Internal tooth bleaching, and· Post-endodontic restorations(5)

Clinical Performances

The latest development of endodontics is remarkable as seen in root canal instrumentation by super-elastic
Ni-Ti rotary ﬁles, diagnosis by cone beam computed tomography, and microendodontics by using a surgicaloperating microscope. In particular, microendodontics has dramatically changed conventional blind endodontics into more predictable endodontics since it allows us to obtain accurate diagnostic information andprovide precise procedures under an illuminated and magniﬁed view. Also, we seek to provide evidence-based
endodontic treatment based on our laboratory and clinical research.
(6)

Publications[Original Articles]
1. Sakaue Y, Domon H, Oda M, Takenaka S, Kubo M, Fukuyama Y, Okiji T, Terao Y. Anti-bioﬁlm and bactericidal eﬀects of Magnolia bark-derived magnolol and honokiol on Streptococcus mutans. Microbiology& Immunology. 2016.01; 60(1); 10-16
2. Yamamoto S, Han L, Okiji T. S-PRG ﬁller extract solutions induce dentinal tubule occulusion and elemental incorporation in ground human dentin The Journal of Japan Endodontic Association. 2016.01;
37(1); 31-37― 47 ―

Oral Health Sciences

Oral Health Sciences

3. Kawashima N, Saito M, Okiji T. Osr2 (odd-skipped related 2) induced diﬀerentiation of hard-tissue forming
cells in mouse dental papilla cells The journal of Japan Endodontic Association. 2016.01; 37(1); 38-44
4. Edanami N, Shigetani Y, Yoshiba K, Hinata G, Yoshiba N, Okiji T. Evaluation of the biocompatibilityof a 4-META containing resin-based root canal sealer in the rat subcutaneous tissue Japanese Journal ofConservative Dentistry. 2016.02; 59(1); 65-73
5. Ishizaki H, Nakajima T, Ito H, Okumura N, Kobayashi T, Uoshima K, Takagi R, Okiji T, Fujii N.
Analysis and safety management of incidents in post-graduate clinical training The Journal of Japanese
Dental Education. 2016.04; 32(1); 29-36
6. Han L, Okiji T. An ion extract obtained from mineral trioxide aggregate induced dentin remineralizationand dentinal tubule occlusion in artiﬁcially demineralized bovine dentin. American Journal of Dentistry.
2016.06; 29(3); 166-170
7. Han L, Yamamoto S, Okiji T. Eﬀect of a prototype S-PRG ﬁller-containing endodontic paste on root
canal dentin: dentinal tubule occlusion and incorporation of elements Japanese Journal of Conservative
Dentistry. 2016.06; 59(3); 279-286
8. Shigetani Y, Ohkura N, Yoshiba K, Ohshima H, Hosoya A, Yoshiba N, Okiji T. GaAlAs laser-induced
pulp mineralization involves dentin matrix protein 1 and osteopontin expression. Oral Diseases. 2016.07;
22(5); 399-405
9. Takafumi Ito, Tomoatsu Kaneko, Yukiko Sueyama, Reika Kaneko, Takashi Okiji. Dental pulp tissue
engineering of pulpotomized rat molars with bone marrow mesenchymal stem cells. Odontology. 2016.11;
10. Komatsu K, Ebihara A, Okiji T. Diﬀerential diagnosis of vertical root fractures using a three-dimensionalbone defect evaluation system Journal of Japan Association of Dental Traumatology. 2016.12; 12(1);
26-35
11. Y Sueyama, T Kaneko, T Ito, T Okiji. Eﬀect of lipopolysaccharide stimulation on stem cell-associatedmarker-expressing cells. Int Endod J. 2016.12;
12. Yamamoto S, Han L, Noiri Y, Okiji T. Evaluation of the Ca ion release, pH and surface apatite formationof a prototype tricalcium silicate cement. International Endodontic Journal. 2016.12; Epub ahead ofprint
13. Uraba S,Ebihara A,Komatsu K,Ohbayashi N,Okiji T. Ability of cone-beam computed tomography todetect periapical lesions that were not detected by periapical radiography: A retrospective assessment
according to tooth group Journal of Endodontics.
[Books etc]
1. Kaneko T, Sueyama Y, Okiji T, Nör JE. Understand Cancer - Research and Treatment. iConcept Press,2016.03 (ISBN : 978-1-922227-386)
2. Okiji T. Case assessment and strategy for endodontics. 2016.05 (ISBN : 978-7-5381-9510-1)[Misc]
1. Nishijo M, Watanabe S, Okiji T. Outcome of orthograde retreatment after faild apicoectomy: use ofmineral trioxide aggregate apical plug 2016.03; 35(3); 203-205
2. Watanabe S, Okiji T. Application of laser to endodontics The Journal of Japan Society for Laser Surgery
and Medicine. 2016.04; 37(1); 37-42
3. Noda S, Watanabe S, Okiji T. A prospective study of the incidence of asymptomatic pulp necrosis following
crown preparation The Quintessence. 2016.06; 35(６); 226-228
4. Kawashima N, Okiji T. Odontoblasts: Specialized hard-tissue-forming cells in the dentin-pulp complex
Congenital Anomalies (Kyoto). 2016.07; 56(4); 144-153
5. Okiji T. Mineral trioxide aggregate: current development and physicochemical properties The Journal of
the Japanese Society for Dental Materials and Devices. 2016.07; 35(4, 5); 237-240― 48 ―

Oral Health Sciences

Oral Health Sciences

6. Hashimoto K, Watanabe S, Okiji T. Treatment outcome of repaired root perforation: a systematic review
and meta-analysis the Quintessence. 2016.10; 35(10); 218-220
7. Sunakawa M. Infection Control in Deentistry Carlisle. 2016.12; 21(3); 1-3[Conference Activities & Talks]
1. Sueyama Y, Kaneko T, Ito T, Okiji T. Eﬀects of lipopolysaccharide for three kinds of dental pulp related stem cells. Universitas Indonesia-Niigata University collaborative symposium 2016.01.09 Lombok,
Indonesia
2. Komatsu K, Ebihara A, Okiji T. Diﬀerential diagnosis of vertical root fractures using a three-dimensionalbone defect evaluation new system. The 6th conference of Japan Association of Dental Traumatology
2016.04.17 Tokyo
3. Ikeda H. Dentin hypersensitivity from the point of view of pulpal nerves and cells -Preconception andtruth-. Federation of Endodontic Association (World Endodontic Congress) 2016.06.03 Cape Town, South
Africa
4. TOKITA Daisuke, NISHIJO Miki, MIYARA Kana, EBIHARA Arata, OKIJI Takash. Analysis of stresses
developed on the root during root canal shaping with diﬀerent reciprocal motions. The 144th JSCD
Meeting 2016.06.09 Utsunomiya, Tochigi
5. Watanabe S, Hongo T, Ide A, Satake K, Yao K, Ebihara A, Okiji T. Diode laser activation of sodium
hypochlorite for lateral root canal soft tissue dissolution. The 144th Meeting of the Japanese Society ofConservative Dentistry 2016.06.09 Utsunomiya
6. Nishijo M, Tokita D, Miyara K, Ebihara A, Okiji T. Evaluation of shaping ability and cyclic fatigue resistance during glide path preparation with prototype automatic root canal preparation system. The144th
Meeting of the Japanese Society of Conservative Dentidtry 2016.06.09 Utsunomiya
7. Yamamoto S, Han L, Okiji T. Evaluation of a prototype S-PRG ﬁller-containing endodontic material:
Dentinal tubule occlusion and element incorporation into the root canal dentin. The144th Meeting of theJapanese Society of Conservative Dentidtry 2016.06.09 Utsunomiya, Tochigi
8. Ohkura N, Yoshiba N, Yoshiba K, Oda Y, Okiji T. Eﬀect of prostaglandin EP4 receptor agonist on
cultured human dental pulp tissue. The144th Meeting of the Japanese Society of Conservative Dentidtry
2016.06.09 Utsunomiya, Tochigi
9. Tazawa K, Ikeda H, Kawashima N, Okiji T. Expression of TRPM8 in human odontoblasts. The 144th
Meeting of the Japanese Society of Conservative Dentistry 2016.06.09 Utsunomiya
10. Ikeda H, Aierken S, Tazawa K, Okiji T. Quantitative evaluation of the eﬀects of etching/whitening materials on enamel permeability. The 144th Meeting of the Japanese Society of Conservative Dentistry2016.06.09 Utsunomiya
11. Fukumori Y, Nishijo M, Tokita D, Miyara K, Ebihara A, Okiji T. Comparative evaluation of mechanicalproperties with diﬀerent tapered nickel-titanium ﬁles -cyclic fatigue and torsional load-. The 144th Meetingof the Japanese Society of Conservative Dentistry 2016.06.09 Utsunomiya
12. Bakhit A, Kawashima N, Yamamoto M, Hashimoto K, Noda S, Nara K, Okiji T. Eﬀects of strontium
ranelate on the mouse dental papillae cells (MDPs). IADR Pulp Biology and Regeneration Group Satellite
Symposium 2016 2016.06.22 Nagoya
13. Hongo T, Watanabe S, Ide A, Satake K, Yao K, Ebihara A, Kobayashi C, Okiji T. Kinetics of vaporized cavitation bubbles during root canal irrigation activated by diode laser. 94th General Session andExhibition of the IADR 2016.06.22 Seoul, Korea
14. Sueyama Y, Kaneko T, Ito T, Okiji T. Lipopolysaccharide induces proliferation and CD146-upregulationof dental pulp stem cell. 94rd General Session and Exhibition of the IADR 2016.06.22 Seoul, Korea
15. Tazawa K, Ikeda H, Kawashima N, Ozawa M, Suda H, Okiji T. Expression of TRPM8 in freshly isolated
human odontoblasts. 94th General Session and Exhibition of the IADR 2016.06.22 Seoul, Korea― 49 ―

Oral Health Sciences

Oral Health Sciences

16. Ikeda H, Suda H, Okiji T. Cold stimulus activates ionic channels in freshly isolated human odontoblasts.
94rd General Session and Exhibition of the IADR 2016.06.22 Seoul, Korea
17. Aramaki O, Kawashima N, Shimada Y, Okiji T, Tagami J . Three-dimensional analysis of Iba1+ macrophages
in human dental pulp using whole mount immunostaining. 94th General Session & Exhibition of the IADR
2016.06.22 Seoul, Korea
18. Ikeda H, Aierken S, Tazawa K, Suda H, Okiji T. Quantitative analysis of changes in human enamel permeability after acid-etching/whitening procedures. IADR Pulp Biology and Regeneration Group Satellite
Symposium 2016 2016.06.26 Nagoya
19. Kawashima N, Noda S, Hashimoto K, Yamamoto M, Okiji T. Properties of dental pulp derived-mesenchymal
stem cells and eﬀects of culture conditions. IADR Pulp Biology and Regeneration Group Satellite Symposium 2016 2016.06.26 Nagoya
20. Yoshiba N, Yoshiba K, Ohkura N, Edanami N, Takeuchi R, Tohma A, Oda Y, Hosoya A, Nakamura
H, Okiji T. Fibrillin-1 microﬁbrils inﬂuence human dental pulp regeneration. IADR Pulp Biology andRegeneration Group Satellite Symposium 2016 2016.06.28 Nagoya
21. Hashimoto K, Kawashima N, Nara K, Noda S, Saito M, Okiji T. Recovery eﬀects of EDTA treatment on
the attachment and diﬀerentiation of mouse dental pulp cells cultured on NaOCl-treated dentin. IADR
Pulp Biology and Regeneration Group Satellite Symposium 2016 2016.06.28 Nagoya
22. Noda S, Kawashima N, Hashimoto K, Aramaki O, Yamamoto M, Okiji T. Eﬀects of conﬂuent culture
condition on dental pulp stem cell surface markers, proliferation, gene expression. IADR Pulp Biology
and Regeneration Group Satellite Symposium 2016 2016.06.28 Nagoya
23. Nara K, Kawashima N, Noda S, Hashimoto K, Bakhit A, Okiji T. Increase of miR21 expression in dental
pulp cells and tissues in response to LPS stimulation. IADR Pulp Biology and Regeneration Group
Satellite Symposium 2016 2016.06.28 Nagoya
24. Ebihara A, Komatsu K, Kawamura J, Tsuruta J, Kinoshita A, Okiji T. Application of ﬂip teaching topreclinical practice of endodontics. The 35th General and Scientiﬁc Meeting of the Japanese Dental
Education Association 2016.07.02 Osaka
25. Kawamura J, Ebihara A, Suzuku N, Okiji T. Application of dental operating microscope to preclinicalpractice of endodontics. The 35th General and Scientiﬁc Meeting of the Japanese Dental Education
Association 2016.07.02 Osaka
26. Kaneko T, Niwano K, Noiri Y, Maeda T, Okiji T. Introduction of the use of Ni-Ti rotary instruments
in preliminary clinical practice of endodontics; root canal preparation using R-phase Ni-Ti rotary instruments. The 35th General and Scientiﬁc Meeting of the Japanese Dental Education Association 2016.07.02
Osaka
27. Komatsu K, Ebihara A, Okiji T. Diﬀerential diagnosis of vertical root fractures using a three-dimensionalbone defect evaluation system: new indexes. The 16th conference of Japan Association of Dental Traumatology 2016.07.16 Kobe
28. Watanabe S, Ide A, Yao K, Satake K, Hongo T, Ebihara S, Kobayashi C, Okiji T. Pressure generated
outside the apical foramen during root canal irrigation activated by diode laser. The 15th Congress of theWorld Federation for Laser Dentistry 2016.07.17 Nagoya
29. Hongo T, Watanabe S, Ide A, Satake K, Yao K, Ebihara A, Okiji T. Kinetics of vaporized cavitation
bubbles during root canal irrigation activated by diode laser. The 15th Congress of the World Federation
for Laser Dentistry 2016.07.17 Nagoya
30. Iino Y, Yoshioka T, Hanada T, Ebihara A, Sunakawa M, Sumi Y, Okiji T. Visualization of substructuresof resected apical root surface by swept-source optical coherence tomography. The 15th Congress of theWorld Federation for Laser Dentistry 2016.07.17 Nagoya
31. Rashed BA, Ebihara A, Iino Y, Yoshioka T, Hanada T, Sunakawa M, Sumi Y, Okiji T. Evaluation of root
canal anatomy of maxillary premolars using swept source optical coherence tomography in comaparison
with dental operating microscope and cone beam computed tomography. The 15th Congress of the World
Federation for Laser Dentistry 2016.07.18 Nagoya― 50 ―

Oral Health Sciences

Oral Health Sciences

32. TOKITA Daisuke,NISHIJO Miki,MAKI Keiichiro,MIYARA Kana,EBIHARA Arata,OKIJI Takashi. Analysis of stresses during root canal shaping with TF Adaptive system. T h e 3 7 t h A n n u a l S c i e n t i
f i c M e e t i n g o f J E A 2016.07.23
33. Nakajima A, Yao K, Ide A, Hongo T, Satake K, Watanabe S, Okiji T. Eﬃcacy of a new ultrasonic device
in removing calcium hydroxide. The 37th Annual Scientiﬁc Meeting of Japan Endodontic Association
2016.07.23 Nagoya
34. Yamamoto S, Han L, Noiri Y, Okiji T. Evaluation of bioactivity on the prototype tricalcium silicate
cement. The 37th Annual Scientiﬁc Meeting of Japan Endodontic Association 2016.07.23 Nagoya
35. Ikeda S,Kim S,Ide A,Satake K, Watanabe S,Okiji T. Cone-Beam Computed Tomography Analysis of Root
Canal Conﬁguration of Maxillary and Mandibular Molars. The 37th Annual Scientiﬁc Meeting of Japan
Endodontic Association 2016.07.23 Nagoya
36. Maki K, Nishijo M, Tokita D, Ebihara A, Okiji T. Change of cyclic fatigue and cantilever bending properties of EndoWave instruments after clinical use . The 37th Annual Scientiﬁc Meeting of Japan Endodontic
Association 2016.07.24 Nagoya
37. Kaneko T, Ito T, Sueyama Y, Okiji T. Immunohistochemical characterization of macrophage-like cells inmesenchymal stem cell-implanted engineered pulp tissues. The 37th Annual Scientiﬁc Meeting of Japan
Endodontic Association 2016.07.24 Nagoya
38. Nishijo M, Maki K, Tokita D, Miyara K, Ebihara A, Okiji T. Shaping ability of ProTaper NEXT driven
by a new automatic root canal preparation device in simulated root canals performed by undergraduate
students. The 37th Annual Scientiﬁc Meeting of Japan Endodontic Association 2016.07.24 Nagoya
39. Fujii M, kawashima N, Okiji T. Induction of HIF1 α in human dental pulp cells by lipopolysaccharide.
The 58th Annual Meeting of Japanese of Association of Oral Biology 2016.08.24 Sapporo
40. Sueyama Y, Kaneko T, Ito T, Okiji T. Implantation of stem cells with endothelial cells accelerates tissue
regeneration. Japanese Association for Dental Research Hatton Award Competition 2016.09.02 Tokyo
41. Okiji T. Defense, repair and regeneration of the dentin/pulp complex: a biological basis for vital pulp
therapy . Penn Endodontic Global Symposium in Japan: Endodontics in Implant Era 2016.09.10 Tokyo
42. Kagaya N, Sawa E, Kobayashi N, Hanaoka S, Kobayashi M, Matsumoto H, Suzuki H. Patient’s attitude
survey to "harmful rumor" of HIV/AIDS. The 33rd Annual Meeting of The Japan Society for Disability
and Oral Hearlth 2016.10.02 Omiya
43. Iino Y, Ebihara A, Yoshioka T, Hanada T, Sunakawa M, Sum Y, Okiji T. Application of optical coherence
tomography in endodontics. The 23rd General Meeting of the Japanese Association for Dental Science2016.10.21 Fukuoka
44. Tazawa K, Ikeda H, Gu J, Okiji T. Human odontoblasts have a ability to detect cold stimulation. The
23rd General Meeting of the Japanese Association for Dental Scicnce 2016.10.21 Fukuoka
45. Okiji T. Vital pulp therapy: biological basis and current concepts. The 18th Joint Scientiﬁc Meeting of
Korean Academy of Conservative Dentistry - Japanese Society of Conservative Dentistry 2016.10.23 Seoul,Korea
46. Nara K, Kawashima N, Noda S, Hashimoto K, Okiji T. MicroRNA21 down-regulates the expression of
inﬂammatory mediators in LPS-stimulated human dental pulp cells via an NF-κ B dependent mechanism.
The 18th Joint Scientiﬁc Meeting of Korean Academy of Conservative Dentistry - Japanese Society ofConservative Dentistry 2016.10.23 Seoul, Korea
47. Kang D, Wada T, Uo M, Okiji T. Evaluation of mechanical properties of an experimental synthetic
tricalcium silicate cement. The 18th Joint Scientiﬁc Meeting of Korean Academy of Conservative Dentistry- Japanese Society of Conservative Dentistry 2016.10.23 Seoul, Korea
48. XU Jing, TOKITA Daisuke, KIMURA Shunsuke, MAKI keiichirou, NISHIJO Miki, MIYARA Kana,
EBIHARA Arata, OKIJI Takashi . Evaluation of mechanical properties of ProTaper Gold and ProTaper
NEXT instruments. 2016.10.26― 51 ―

Oral Health Sciences

Oral Health Sciences

49. Kuramoto M, Kawashima N, Bakhit A, Nara K, Fujii M, Noda S, Hashimoto K, Saitoh M, Okiji T. Eﬀects
of mineral trioxide aggregate on mouse dental papillae cells treated with lipopolysaccharide. The 145th
Meeting of the Japanese Society of Conservative Dentistry 2016.10.27 Matsumoto
50. Yasumiko Wakabayashi, Nobuyuki Kawashima, Alamuddin Yassin Bakhit, Kentaro Hashimoto, Sonoko
Noda, Keisuke Nara, Takashi Okiji. Cytotoxicity and osteoblast-diﬀerentiation induction of a prototype S-PRG ﬁller-containing endodontic sealer. The japanese society of conservative dentistry 2016.10.28
Matsumoto City, Nagano Prefecture
51. Sunakawa M. Sterilization and disinfection methods of the used instruments in the dental clinic -actuals
at the dental hospital, Tokyo Medical and Dental University-. Instrument Management & Infection
Prevention Synposium 2016.10.30 Tokyo
52. Watanabe S. 30 Evidences quick update. Innovative Zahnmedizin 2016.11.06 Tokyo
53. Kawashima N, Hashimoto K, Okiji T. Rescue of mouse dental papillae cell-attachment to sodium hypochloritetreated dentin disks by EDTA treatment. The 49th Scientiﬁc Meeting of Korean Academy of Endodontics
2016.11.19 Seoul, Korea
54. Gu B, Kaneko T, Sueyama Y, Sone PP, Okiji T. Immunohistochemical characterization of M2 macrophages
in a rat experimental model of coronal pulp tissue engineering. The 59th Symposiumu of The JapaneseSociety of Microscopy 2016.11.19 Tokyo
55. Ikeda H. Microcirculation of the dental pulp. Symposium on Pulp Biology in Taiwan 2016.12.27 Taipei,
Taiwan[Awards & Honors]
1. Best oral presentation award 2nd prize (Evaluation of root canal anatomy of maxillary premolars using
swept source optical coherence tomography in comparison with dental operating microscope and cone
beam computed tomography, Rashed BA), The 15th Congress of the World Federation for Laser Dentistry,
2016.07
2. Best poster award (Rescue of mouse dental papillae cell-attachment to sodium hypochlorite-treated dentin
disks by EDTA treatment, Nobuyuki Kawashima), The 49th Scientiﬁc Meeting of Korean Academy ofEndodontics, 2016.11― 52 ―

Oral
Oral Health
Health Sciences
Sciences

Removable Partial Prosthodontics

Professor - Noriyuki Wakabayashi
Associate professor - Kenji Fueki
Junior associate professor - Takeshi Ueno
Assisstant professors- Ichiro Minami, Juro Wadachi, Shusuke Inukai, Eiko Kohno, Junnichiro Wada, Natsuko Murakami
Hospital staﬀ
- Atsushi Takaichi, Natsuki Suzuki, Maho Shiozawa, Yuki Arai, Yuka Kajima, Chiaki Tsutsumi, Yasuha Nogawa
Graduate students- Keigo Isoshima, Hideaki Inagawa, Yuka Inamochi, Keiichiro Uchikura, Hisami Okawara,
Kittikundecha Nuttaphon,Hiroki Saito, Yuya Satokawa, Erina Seki,
Kensuke Takakusaki, Tenhaku Tan, Kazutoshi Nakamura, Tomiharu Nagayama,
Gen Nabeshima, Yoko Hayashi, Hironari Hayama, Masahiro Hirasawa,
Toshiki Yamazaki, Chie Yoshihara, Chie Watanabe(1)OutlineThe Department of Removable Partial Prosthodontics specializes in removable partial denture treatment, which
is one of the major disciplines of clinical dentistry. The department has taken the baton from the First Department of Prosthodontics and the Department of Masticatory Function Rehabilitation.
Our objectives are to enhance the art and science of removable prosthodontics for the management of various
oral conditions associated with tooth loss, from a single missing tooth to a single remaining tooth, in the maxillary and/or mandibular arch.

(2)

Research

1. Function and Physiology in Partial Denture Wearers
2. Optimization of Partial Denture Design based on Stress Analysis
3. Development and Applications of New Prosthodontic Biomaterials
4. Biology of Oral Tissues in Denture Wearers
5. Epidemiology and Education for Removable Partial Prosthodontics(3)

Education

School of DentistryYear 1Introduction to Dentistry― 53 ―

Oral
HealthSciences
Oral
HealthSciencesYear 3Tooth Carving
Introduction to Clinical Dentistry
Observation and assistance at clinic term Ⅰ and II
Basic Occlusal Reconstruction
Introduction to Research article writing and Patent acquisition
Year 4
Research Project
Removable Partial Prosthodontics
Advanced knowledge and skill with occlusion
Experience learning of dental practiceYears 5 and 6
Case study
Dental Practice and Clinical Practicum for Comprehensive Patient Care (Clinical Internship)
School of Oral Health Care Sciences, School of Oral Health Care Sciences
Year 2 Prosthodontics
Years 3 and 4 Practice for Dental Hygiene Care
School of Oral Health Care Sciences, Course for Oral Health EngineeringYear 2 Removable Prosthodontics Technology(4)

Lectures & Courses

The Department of Removable Partial Prosthodontics oﬀers a variety of educational courses pertaining to thespecialized clinical management of tooth loss, primarily for undergraduate students at the School of Dentistry.
The courses include lectures, tutorials, hands-on sessions, clinical simulations, and clinical internship instructions.
The ability to perform clinical operations on patients with tooth loss, including the skills required for medical
interviews, oral examinations, diagnosis, decision making, impression making, bite registration, denture design
and technology, and denture delivery and maintenance, is essential for clinical dental professionals. The objectives of our courses are to provide learning and training in contemporary removable prosthodontics and enhance
the knowledge and skills of students to help them develop and ﬂourish in their future career as dental and oral
health professionals.

(5)

Clinical Services & Other Works

All faculty members of the Department of Removable Partial Prosthodontics are assigned to treat patients at the
Prosthodontics department in the Dental Hospital of the Tokyo Medical and Dental University. While the rateof tooth retention has increased in comparison with previously reported rates, the number of patients in need ofa removable partial denture has increased. Our specialists primarily work on advanced cases that are referredfrom other departments and outside the hospital. The departmental mission also includes the development andapplication of new prosthodontic materials, their clinical trial, and the spread of novel and inventive knowledge
to the community.
(6)

Clinical Performances

The department specializes in removable partial prosthodontics and aims to restore missing teeth and associatedoral tissues; improve physiological functions such as occlusion, mastication, swallowing, and speech; maintainnormal oral sensation; and restore the original appearance of individual patients.

― 54 ―

Oral
Oral Health
Health Sciences
Sciences

Treatment modalities, materials, and denture design are all based on the case history and chief complaint of
the patients. The ultimate goal is to improve the oral health-related quality of life of patients.
(7)

Publications[Original Articles]
1. Wada K, Ikeda E, Wada J, Inoue G, Miyasaka M, Miyashin M. Wear characteristics of trimethylolpropane
trimethacrylate ﬁller-containing resins for the full crown restoration of primary molars. Dental Materials
Journal. 2016; 35(4); 585-593
2. Matsuura H, Yamamoto T, Wada J, Inukai S, Hideshima M. A detection method using phonetic segments
for the inﬂuence of fatigue on speech 2016; J99-A(9); 351-360
3. Kajima Y, Takaichi A, Yasue T, Doi H, Takahashi H, Hanawa T, Wakabayashi N.. Evaluation of theshear bond strength of dental porcelain and the low magnetic susceptibility Zr-14Nb alloy. Journal of the
Mechanical Behavior of Biomedical Materials. 2016.01; 53; 131-141
4. Yuk Kajima, AtsushiTakaichi, Takayuki Nakamoto, Takahiro Kimura,Yoshiaki Yogo, Maki Ashida, Hisashi
Doi, Naoyuki Nomura, Hidekazu Takahashie, Takao Hanawa, Noriyuki Wakabayashi. Fatigue strengthof Co-Cr-Mo alloy clasps prepared by selective laser melting. Journal of the mechanical behavior of
biomedical materials. 2016.06; 59; 446-458
5. Tsunglin James Lee, Takeshi Ueno, Naoyuki Nomura, Noriyuki Wakabayashi, Takao Hanawa. TitaniumZirconium Binary Alloy as Dental Implant Material: Analysis of the Inﬂuence of Compositional Change
on Mechanical Properties and In Vitro Biologic Response. Int J Oral Maxillofac Implants. 2016.06; 31(3);547-554
6. K Fueki, Y Igarashi, Y Maeda, K Baba, K Koyano, K Sasaki, Y Akagawa, T Kuboki, S Kasugai, N R
Garrett. Eﬀect of prosthetic restoration on masticatory function in patients with shortened dental arches:a multicentre study. J Oral Rehabil. 2016.07; 43(7); 534-542
7. Yuki Arai, Kazuhiro Aoki, Yasuhiro Shimizu, Yasuhiko Tabata, Takashi Ono, Ramachandran Murali, Setsuko Mise-Omata, Noriyuki Wakabayashi. Peptide-induced de novo bone formation after tooth extraction
prevents alveolar bone loss in a murine tooth extraction model. Eur. J. Pharmacol.. 2016.07; 782; 89-97
8. Kohji Nagata, Sufyan K. Garoushi, Pekka K. Vallittu, Noriyuki Wakabayashi, Hidekazu Takahashi, Lippo
V. J. Lassila. Fracture behavior of single-structure ﬁber-reinforced composite restorations Acta Biomaterialia Odontologica Scandinavica. 2016.07; 2(1); 118-124
9. Hiroshi Matsuura, Yuji Hojo, Koichi Sawasaki, Junichiro Wada, Shusuke Inukai, Masayuki Hideshima.
An E-Learning System of Phrase Accent and Intonation for Learners of Japanese, Based on Phonetic
Segment Labels and Fundamental Frequency 2016.08; 16; 29-39
10. H Kumagai, K Fueki, E Yoshida-Kohno, N Wakabayashi. Factors associated with mucosal pain in patientswith partial removable dental prostheses. J Oral Rehabil. 2016.09; 43(9); 683-691
11. Takeshi Ueno, Takayuki Ikeda, Naoki Tsukimura, Manabu Ishijima, Hajime Minamikawa, Yoshihiko
Sugita, Masahiro Yamada, Noriyuki Wakabayashi, Takahiro Ogawa. Novel antioxidant capability oftitanium induced by UV light treatment. Biomaterials. 2016.11; 108; 177-186
12. Teisuke Akimoto, Takeshi Ueno, Yusuke Tsutsumi, Hisashi Doi, Takao Hanawa, Noriyuki Wakabayashi.
Evaluation of corrosion resistance of implant-use Ti-Zr binary alloys with a range of compositions. J.
Biomed. Mater. Res. Part B Appl. Biomater.. 2016.11;
13. Yasuha Ikawa, Ayako Mochizuki, Keisuke Katayama, Takafumi Kato, Minako Ikeda, Yuka Abe, Shiro
Nakamura, Kiyomi Nakayama, Noriyuki Wakabayashi, Kazuyoshi Baba, Tomio Inoue. Eﬀects of citalopram on jaw-closing muscle activity during sleep and wakefulness in mice. Neurosci. Res.. 2016.12; 113;
48-55

― 55 ―

Oral
HealthSciences
Oral
HealthSciences[Books etc]
1. Standard Partial Denture. 2016.01 (ISBN : 978-4-7624-2656-8)
2. Wada J, Takaichi A, Wakabayashi N. Partial Denture Decision Making. Ishiyaku Publishing, 2016.02(ISBN : 978-4-263-44460-3)
3. Masaru Yatabe, Kaori Maehata. Denture Q&A. 2016.09 (ISBN : 978-4-88510-358-2)[Misc]
1. Hisatomo Kondo,Shogo Ozawa,Takashi Sawase,Atsuro Yokoyama,Hideshi Sekine,Takeo Maida,Yasunori
Ayukawa,Tamaki Nakano,Takayasu Kubo,Ryuji Hosokawa,Yoritoki Tomotake,Hirofumi Kido,Morio Ochi,Makoto
Shiota,Masahiko Ozeki,Masahiro Nishimura, Yoshinobu Maeda,Hideki Aita,Katsushi Tamaki,Kenji Fueki,Hiroaki
Tsukasaki,Takahiro Ono,Yasuyuki Matsushita,Yoshizo Matsuka,Hajime Minakuchi,Rika Kuwatsuru,Shuichiro
Yamashita,Tomoaki Iizuka,Kazuyoshi Baba,Masanori Fujisawa,Kiyoshi Koyano,Hirofumi Yatani. Occlusal
consideration for ﬁxed implant prosthesis in mandibular posterior region Ann Jpn Prosthodont Soc.
2016.01; 8(1); 1-8
2. Katsushi Tamaki, Shoichi Ishigaki, Takumi Ogawa, Hitoshi Oguchi, Takafumi Kato, Takeshi Suganuma,
Atsushi Shimada, Shinsuke Sadamori, Yoshihiro Tsukiyama, Youji Nishikawa, Shin-Ichi Masumi, Taihiko
Yamaguchi, Hideki Aita, Takahiro Ono, Hisatomo Kondo, Hiroaki Tsukasaki, Kenji Fueki, Masanori
Fujisawa, Yoshizo Matsuka, Kazuyoshi Baba, Kiyoshi Koyano. Japan Prosthodontic Society position
paper on "occlusal discomfort syndrome". J Prosthodont Res. 2016.07; 60(3); 156-166
3. Natsuko Murakami, Junichiro Wada, Noriyuki Wakabayashi. Q11_Why is tooth preparation necessary
when making a partial denture? DHstyle Special issue Denture Q&A. 2016.09; 10(10); 34-35
4. Natsuko Murakami, Junichiro Wada, Noriyuki Wakabayashi. Q12_What is a snap impression? DHstyleSpecial issue Denture Q&A. 2016.09; 10(10); 36-37
5. Natsuko Murakami, Junichiro Wada, Noriyuki Wakabayashi. Q13_What is a study model? DHstyleSpecial issue Denture Q&A. 2016.09; 10(10); 38-39
6. Natsuko Murakami, Junichiro Wada, Noriyuki Wakabayashi. Q14_What are the points of taking denture
impression? DHstyle Special issue Denture Q&A. 2016.09; 10(10); 40-41
7. Natsuko Murakami, Junichiro Wada, Noriyuki Wakabayashi. Q15_How to care for gag reﬂex patients
DHstyle Special issue Denture Q&A. 2016.09; 10(10); 42-43
8. Natsuko Murakami, Junichiro Wada, Noriyuki Wakabayashi. Q16_How to pour dental plaster models?
DHstyle Special issue Denture Q&A. 2016.09; 10(10); 44-45
9. Junichiro Wada, Natsuko Murakami, Noriyuki Wakabayashi. Q17_What should we check when bitetaking? 2016.09; 10(10); 46-47
10. Junichiro Wada, Natsuko Murakami, Noriyuki Wakabayashi. Q18_What should we check when we try-in
the RPD? 2016.09; 10(10); 48-49
11. Junichiro Wada, Natsuko Murakami, Noriyuki Wakabayashi. Q19_What should we do at the day of RPD
placement? 2016.09; 10(10); 50-51
12. Fueki K. Non-metal clasp dentures: More evidence is needed for optimal clinical application. J ProsthodontRes. 2016.10; 60(4); 227-228
13. Akihito Uezato, Mitsuhiro Enomoto, Meiyo Tamaoka, Mizue Hobo, Shusuke Inukai, Masayuki Hideshima,
Yasunari Miyazaki, Toru Nishikawa and Kazuyoshi Yagishit. Shorter sleep onset latency in patients
undergoing hyperbaric oxygen treatment Psychiatry and Clinical Neurosciences. 2016.12; 71(1); 73-74― 56 ―

Oral
Oral Health
Health Sciences
Sciences[Conference Activities & Talks]
1. Yukihiko Tamura, Setsuko Mise, Yasutaka Sugamori, Md. Zahirul Haq Bhuyan, Mariko Takahashi,
Tomoki Uehara, Yuki Arai, Michiyo Miyashin, Noriyuki Wakabayashi, Kazuhiro Aoki. A pharmacological role of metallothionein in zinc-treated cartilage-progenitor cells. APFP 2016(The 13th Asia Paciﬁc
Federation of Pharmacologists Meeting) 2016.02.01 The Berkeley Hotel Pratunam,Bangkok (Thailand)
2. Masuru Yatabe. Non-metal clasp denture 2016. 2016.02.07
3. Wakabayashi N. Future Perspectives of Dental Education and Research in Globalized Society. Programme
for visit from Tokyo Medical and Dental University (TMDU) 16-18 March 2016 2016.03.15 Gothenburg,
Sweden4. Yogo Y,Kajima Y,Takaichi A,Nakamoto T,Doi H,Takahashi H,Nomura N,Wakabayashi N. The eﬀect ofheat treatment on microstructure and mechanical properties of Co-Cr-Mo alloys fabricated by selective
laser melting process. The 67th General Session of the Japanese Society for Dental Materials and Devices
2016.04.17 Fukuoka
5. Mochizuki A, Ikawa Y, Kato T, Katayama K, Nakamura S, Nakayama K, Baba K, Inoue T. The eﬀects
of Citalopram on the modulation of masseter muscle activity during non-REM sleep . 57th Scientiﬁc
Meeting of the Japanese Society of Stomatognathic Function 2016.04.23 Saitama
6. Yoshihara C, Ueno T, Chen P, Tsutsumi Y, Hanawa T, Wakabayashi N. Inverse behavior between osteoblasts and ﬁbroblasts on carbon-coated titanium surfaces. 10th World Biomaterials Congress (WBC2016)2016.05.17 Montreal,Canada
7. Akimoto T, Ueno T, Tsutsumi Y, Doi H, Hanawa T, Wakabayashi N. The Corrosion Resistance of TiZr Binary Alloy with Compositional Change. 10th World Biomaterials Congress (WBC2016) 2016.05.17
Montreal,Canada
8. Ueno T, Wakabayashi N, Ogawa T. . An anti-oxidant property of titanium induced by ultraviolet photoactivation. . The 10th World Biomaterials Congress (WBC) 2016.05.22 Montreal, Canada
9. Masaru Yatabe. Non-metal clasp denture: from designing to maintenance. 2016.05.22
10. Hiroyuki Ishiyama, Shusuke Inukai, Akira Nishiyama, Masayuki Hideshima, Shuhei Nakamura, Chisato
Ida, Yuko Mitsuma, Shota Hayashi, Akihito Uesato, Toshihide Fujie, Meiyo Tamaoka, Yasunari Miyazaki,
Masahiko Shimada, Noriyuki Wakabayashi . Eﬀect of jaw-opening exercise on prevention of temporomandibular disorders pain associated with oral appliance therapy and compliance in obstructive sleep
apnea patients: A randomized, double-blind, placebo-controlled trial.. Japanese Society of Sleep Researchthe 41th Annual Meeting 2016.07.07 Tokyo
11. Current status of CPAP therapy for OSAS in late stage elderly at our hospital. 2016.07.07
12. Practice and practical experience of OA therapy for dental clinic for sleep disorders. 2016.07.07
13. Wada J,Hideshima M, Inukai S, Matsuura H, Wakabayashi N. Inﬂuence of shape of dental arch and palate
on the accuracy of speech production disturbed by major connector of maxillary removable partial denture.
The 125th Annual Meeting of the Japan Prosthodontic Society 2016.07.09 Kanazawa
14. In vitro analysis of two body wear of CAD/CAM machinable ceramics . 2016.07.09
15. Kumagai H, Fueki K, Kohno E, Wakabayashi N. A structural equation model relating mucosal pain andsubjective masticatory function in patients with removable partial dentures. . The 125th Annual Meetingof the Japan Prosthodontic Society 2016.07.09 Kanazawa
16. Fueki K, Kohno E, Yatabe M, Wakabayashi N. Eﬃcacy of Non-metal Clasp Dentures with Metal Framework: A Randomized Cross-over Trial. 125th JPS general meeting 2016.07.10 Kanazawa
17. Takaichi A,Kajima Y,Yogo Y, Takahashi H, Wakabayashi N. The heat treatment of Co-Cr-Mo alloys
fabricated by selective laser melting process. The 125yh annual meeting of the japan prosthodontic
society 2016.07.10 Kanazawa
18. Watanabe C, Wada J, Mizutani K, Watanabe H, Wakabayashi N. The eﬀect of removable partial denture
placement on periodontal structures of abutment teeth in patients of periodontitis: A digital subtraction
analysis. The 125th Scientiﬁc Meeting of Japan Prosthodontic Society 2016.07.10 Kanazawa― 57 ―

Oral
HealthSciences
Oral
HealthSciences

19. C Tsutsumi, K Takakusaki, M Hirasawa, N Wakabayashi. Reduction of Candida bioﬁlm adhesion byincorporation of prereacted glass ionomer ﬁller in denture base resin. 2016.07.10
20. Kajima Y, Takaichi A, Wakabayashi N. The change of magnetic susceptibility and density in dental alloyaﬀect the range of artifact. The 125yh annual meeting of the japan prosthodontic society 2016.07.10
Kanazawa
21. Wakabayashi N.. Education and Research in Tokyo Medical and Dental University. 2016.08.02 Boston
University School of Dental Medicine, 7th Floor, Room G-718
22. Ikawa Y, Mochizuki A, Kato T, Katayama K, Abe Y, Nakamura S, nakayama K, Baba K, Wakabayashi
N, Inoue T. The eﬀects of Citalopram on the modulation of masseter muscle activity during non-REM
sleep. The 58th Annual Meeting of Japanese Association for Oral Biology 2016.08.26 Sapporo
23. K.takakusaki, C.tsutsumi, M.hirasawa, K.takakuda, K.fueki, N.wakabayashi. Eﬀect of S-PRG ﬁller content on C.albicans adherence and mechanical propertys of tissue conditioner. Japanese society for bacteriology 2016.10.06 Tokyo
24. A.Wirianski, R.Harakawa, CC.Peck, GM.Murray, I.Minami. Jaw Muscle Electromyographic Activity
During Chewing is Altered by Isometric Resistance Jaw Exercises.. 23rd General Meeting of the Japanese
Association for Dental Science. 2016.10.22 Hakata, Fukuoka.
25. Yuki Arai, Kazuhiro Aoki, Noriyuki Wakabayashi. Peptide-induced de novo bone formation after tooth
extraction prevents alveolar bone loss. The 5th Tri-University Consortium on Oral Science and Education
2016.10.27 Peking University
26. Ogawa M, Koyanagi T, Takeuchi Y, Katagiri S, Ikawa T, Takeuchi S, Sekiuchi T, Arai Y, Kazama R,
Wakabayashi N, Izumi Y. Evaluation of palatal mucosal thickness measurements using CBCT. 145th
Meeting of the Japanese Society of Conservative Dentistry 2016.10.28 Matsumoto
27. Ogawa M, Koyanagi T, Takeuchi T, Katagiri S, Ikawa T, Takeuchi S, Sekiuchi T, Arai Y, Kazama R,
Wakabayashi N, Izumi Y. Evaluation of palatal mucosal thickness measurements using CBCT. 145th
Meeting of the Japanese Society of Conservative Dentistry 2016.10.28 Matsumoto
28. Kajima Y, Takaichi A, Kittikundecha N, Nakamoto T, Doi H, Takahashi H, Nomura N, Hanawa T,
Wakabayashi N. Eﬀects of heat treatment on the microstructure and mechanical property of selective
laser melted Co–Cr–Mo alloys. . International Dental Materials Congress 2016 (IDMC 2016) 2016.11.05
Bali (Indonesia)
29. Takaichi A, Kajima Y, Kittikundecha N, Nakamoto T, Nomura N, Takahashi H, Hanawa T, and Wakabayashi N . Eﬀects of support structure on the fatigue strength of selective laser melted Co-Cr-Mo clasps.
International Dental Materials Congress 2016 (IDMC2016) 2016.11.06 Bali, Indonesia
30. Inagawa H, Suzuki N, Hachikawa M, Wakabayashi N. Conversion to bone Young’s modulus from CT values:
Validity assessment in mouse maxilla.. Scientiﬁc Meeting of the Kanetsu Branch of Japan Prosthodontic
Society Niigata, Japan 2016.11.06 Niigata
31. Yuka Inamochi, Kenji Fueki, Masato Taira, Noriyuki Wakabayashi . Adaptive change in chewing-related
brain activity while wearing a palatal plate: An fMRI study. The 81th Annual Meeting of The Stomatological Society, Japan 2016.11.26 Tokyo Medical and Dental University
32. Kenji Fueki. Clinical evidence for non-metal clasp denture. Prostho’16 2016.12.10 Tokyo[Awards & Honors]
1. DENTSPLY Merit Award, Japan Prosthodontic Society , 2016.07
2. International Poster Award Presentation, The Japanese Association for Dental Science., 2016.10― 58 ―

Oral
Oral Health
Health Sciences
Sciences

Oral Implantology and Regenerative Dental Medicine

Professor and Chair
Shohei KASUGAI
Associate Professor
Makoto SHIOTA
Assistant Professor(Lecturer) Noriko TACHIKAWA, Shinji KURODA
Assistant Professor
Hidemi NAKATA
Medical Staﬀs:
Kazuhiro KON, Masaki FUJII, Takayuki MIYAHARA, Tokuo AKINO,
Masahiro SHIMOGISHI, Masahiro ISHIWATA, Chiharu IMAKITA, Maiko Yamamoto

Graduate Students:
Yu YAMASHITA, Taiji HAMADA, Masaki SHIBASAKI, Emi OKADA, DING Lin, NGUYEN Vo Ngoc Trang, BOOSAN

Graduate Research Students:
Shuichi KOYAMA, Tadamasa YOSHIDA, Toshimitsu SHIGEMATSU, Toshihiko MORIKAWA,
Kazuhiko INOUE, Takeshi WATANABE, Arihiro IWATA, Narumi SATO,
Gou INOUE, Seiji OHARA, Junya HAMAGUCHI,
Dotetsu TAKONAI, Yoshitaka TANABE, Maho AKATSUKA,
Munemitsu MIYASAKA, Akihiro Takahashi, Kento TAIRA, You-kyoung KIM, Mizuki SATO, Yoko Oba, Chang Kuangs
Resident:
Kaho TAMURA

Registered Residents:
Toshifumi KOJIMA, Kensuke FUKUTOMI, Reina SHIBUYA, Aoi SAKUYAMA, Kazuhisa TSURUMI, Ai KOBAYASHI
Clinical Professor (Faculty of Dentistry)
Hideaki KATSUYAMAClinical Visiting Associate Professor
Koji HAGINO
Clinical Visiting Associate Professor
Tsuneji OKADA
Adjunct Assistant Professor (Faculty of Dentistry):
Maho KON, Yuki DATE, Akiko FURUICHI, Hiroshi KOBAYASHI,
Daisuke SATO, Shang GAO
Adjunct Assistant Professor (Dental Hospital):
Hisatomo KONDO, Hidemichi KIHARA, Toru KANAI(1)

Research

Materials and structures of dental implant prostheses
Implant design and surface modiﬁcation of dental implant
Dental implant and its surrounding tissues
Regeneration of soft tissues
Regeneration of bone

― 59 ―

Oral
HealthSciences
Oral
HealthSciences

Adipose-derived stem cells for osteogenesis(2)

Lectures & Courses

Currently, oral rehabilitation with dental implant is very eﬀective and predictable. It is absolutely important
for the dental student to understand dental implant treatment compared to other modalities. Nine hours
lectures for the 5th year dental students are the introduction part. Each of these students has a chance to see
patient examination process and several steps of treatment planning for half a day in the dental implant clinic.
Furthermore, each of the 6th year students have a chance to see surgical procedures, prosthodontic treatments
and maintenance procedures. In the residential program, we accepted 9 dentists and teach them more advanced
contents of dental implant treatment.
In the doctoral course of Implantology Biomaterial sciences, structural engineering, anatomical structures,
diagnosis and technical innovations are overviewed. In the doctoral course of Regenerative Dental Medicine,
tissue engineering concept, regeneration of soft tissue and bone and recent technological advancements in theseﬁeld are overviewed.
(3)

Clinical Services & Other WorksIn Dental Implant Clinic in the dental hospital, we treat partially or fully edentulous patients with dental
implants. If soft tissue management and/or bone augmentation procedures are required, we also do thesesurgeries. Number of patients in Dental Implant Clinic is increasing every year and approximately 120 patients
per day are treated, which is extremely over our capacities. Approximately 2,000 implants were installed in2012. Patients with some clinical problems, who are treated in other clinics, are increasing and this is a great
concern.
(4)

Publications[Original Articles]
1. You-kyoung KIM, Hidemi NAKATA, Maiko YAMAMOTO, Munemitsu MIYASAKA, Shohei KASUGAI,*Shinji KURODA. Osteogenic Potential of Mouse Periosteum-derived Cell Sorted for CD90 in vitro andin vivo STEM CELLS Translational Medicine. 2016; 5; 1-8
2. Hidemi Nakata, Maiko Yamamoto, Emi Okada, Tomoko Nagayama, Shohei Kasugai, *Shinji Kuroda.
Osteogenic potential of adipose-derived macrospheroids cocultured with CD11b+ monocytes International
Journal of Oral and Maxillofacial Implant. 2016;
3. Hidemi Nakata, *Shinji Kuroda, Noriko Tachikawa, Emi Okada, Maho Akatsuka, Shohei Kasugai, Hisatomo
Kondo. Histological and micro-computed tomographic observations after maxillary sinus augmentation
with porous hydroxyapatite alloplasts: a clinical case series SpringerPlus. 2016;
4. Madi M, Zakaria O, Ichinose S, Kasugai S. Eﬀect of induced periimplantitis on dental implants with andwithout ultrathin hydroxyapatite coating. Implant Dentistry. 2016; 25(1); 39-46
5. Hidemi Nakata, Shinji Kuroda, Noriko Tachikawa, Munemitsu Miyasaka, Kenya Yoneda, Hisatomo
Kondo, Shohei Kasugai. Clinical outcomes of aesthetic and functional rehabilitation with dental implants
in patients with alveolar cleft Journal of Tissue Science & Engineering. 2016; 6(160);
6. Yu Yamashita, Kang Chen, Shinji Kuroda, Shohei Kasugai. Stability of Platelet-rich Fibrin in vivo:
Histological Study in Rats Journal of Oral Tissue Engineering. 2016; 14(2); 83-90
7. Sato D, Kanazawa M, Kim YK, Yokoyama S, Omura Y, Ozeki M, Minakuchi S, Kasugai S, Baba K.
Immediate loading of two freestanding implants placed by computer-guided ﬂapless surgery supporting amandibular overdenture with magnetic attachments. J Prosthodont Res. 2016.01; 60(1); 54-62
8. Trang N Nguyen Vo, Jia Hao, Josh Chou, Masamitsu Oshima, Kazuhiro Aoki, Shinji Kuroda, Boosana
Kaboosaya, Shohei Kasugai. Ligature induced peri-implantitis: tissue destruction and inﬂammatory
progression in a murine model. Clin Oral Implants Res. 2016.01;― 60 ―

Oral
Oral Health
Health Sciences
Sciences

9. Kei Fuchigami, Motohiro Munakata, Takaaki Kitazume, Noriko Tachikawa, Shohei Kasugai, Shinji Kuroda.
A diversity of peri-implant mucosal thickness by site Clinical Oral Implants Research. 2016.02;
10. Omura Y, Kanazawa M, Sato D, Kasugai S, Minakuchi S. Comparison of patient-reported outcomes
between immediately and conventionally loaded mandibular two-implant overdentures: A preliminary
study. J Prosthodont Res. 2016.07; 60(3); 185-192
11. Sato M, Chen P, Tsutsumi Y, Shiota M, Hanawa T, Kasugai S . Eﬀect of strontium ions on calciﬁcationof preosteoblasts cultured on porous calcium- and phosphate-containing titanium oxide layers formed by
micro-arc oxidation Dent. Mater. J.. 2016.07; 35(4); 627-634
12. Kazuhiro Kon, Makoto Shiota, Maho Ozeki, Shohei Kasugai. The eﬀect of graft bone particle size onbone augmentation in a rabbit cranial vertical augmentation model: a microcomputed tomography study.
Int J Oral Maxillofac Implants. 29(2); 402-406[Conference Activities & Talks]
1. Emi Okada, Hidemi Nakata, Shohei Kasugai, Shinji Kuroda. Short-term exposure of bisphosphonates
may enhance osteoblast diﬀerentiation through osteoclasts. The Annual Meeting of the Academy of
Osseointegration 2016.02.18 San Diego, CA, USA
2. M. Kanazawa, M. Iwaki, D. Sato, Y. Omura, A. Miyayasu, S. Kasugai, S. Minakuchi.. Immediate Loadingof two-implants mandibular overdentures: 4-years prospective study.. 94th General Session & Exhibitionof the IADR 2016.06 Seoul, Republic of Korea
3. Asami Mari,Miyayasu Anna,Kanazawa Manabu,Takeshita Shin,Tanoue Mariko,Sato Daisuke,Oda Ken,Shimizu
Haruki,Morisawa Masayuki,Kasugai Shohei,Minakuchi Shunsuke. Cost-eﬀectiveness analysis of mandibular single-implant overdentures and conventional complete dentures Study protocol for a randomized controlled clinical trial . The 125th Scientiﬁc Meeting of Japan Prosthodontic Society 2016.07.09 Kanazawa
4. Shimada Ryo, Kanazawa Manabu, Miyayasu Annna, Tanoue Mariko, Sato Daisuke, Kusumoto Yuriko,
Abe Yuka, Yokowama Sawako, Baba Kazemi, Minakuchi Syunnsuke. Prospective clinical trial of IARPD
with short implants -research protocol- . 2016.11.06 gihu

― 61 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

Plastic and Reconstructive Surgery

Professor: Mutsumi Okazaki
Junior Associate Professor: Hiroki Mori
Assistant Professor (Hospital Staﬀ): Noriko Uemura
Project Assistant Professor（Hospital Staﬀ）：Kentaro Tanaka
Graduate Student: Aki Takada, Takuya Higashino, Tsutomu Homma, Satoshi Usami,
Mayuko Hamanaga, Nobuko Suesada(1)

Research

Basic research
1. A mechanism and prophylaxis of the post-inﬂammatory pigmentation
2. A scarless wound healing
3. The blood circulation study of the ﬂap using indocyanine green
4. Donor speciﬁcity on various ﬂaps or full thickness skin
Clinical research
1. Sensory recovery and contour prediction in the breast reconstruction
2. The algorithmic development and the evaluation of various reconstructions in the skull base reconstruction
3. The development of reconstructive method after an oral cavity / pharyngeal cancer resection - Aiming at
the functional preservation
4. The classiﬁcation of the symptom and static and dynamic reconstruction of the facial paralysis
5. A classiﬁcation and the algorithmic development in blepharoptosis surgery
6. The objective evaluation for the ischemic limb and therapeutic strategy utilizing wound healing mechanism
7. The prospective studies about the color reproducibility of the medical tattoo in the nipple areola reconstruction(2)

Education

Plastic surgery is a specialized branch of surgery concerned with the repair of deformities and the correctionof functional deﬁcits. The specialty of plastic surgery covers a wide range of procedures, and unlike othermedical specialties which concentrate on one particular area of the body, plastic surgeons are involved in thereconstruction and remolding of nearly all external body structures.

(3)

Clinical Performances

We cover the whole ﬁeld of plastic surgery. In particular, we deal with the following ﬁeld; congenital anormaly(cleft lip and palate, microtia blepharoptosis or polydactyly etc), LASERs, cutaneous malignant tumor, skinulcer, breast reconstruction, head and neck reconstruction, facial palsy, axillary osmidrosis.

― 62 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction(4)

Publications[Original Articles]
1. Mori H, Uemura N, Okazaki M, Nakagawa T, Sato T. Nipple malposition after nipple-sparing mastectomy
and expander–implant reconstruction Breast Cancer. 2016; 23; 740-744
2. Uemura N, Okazaki M, Mori H. Anatomical and histological study to determine the border of sole skinSurgical and Radiologic Anatomy. 2016; 38(7); 767-773
3. Tanaka K, Okazaki M, Homma T, Yano T, Mori H. Bilateral inferior alveolar nerve reconstruction witha vascularized sural nerve graft included in a free ﬁbular osteocutaneous ﬂap after segmental mandibulectomy Head & Neck. 2016; 38(5); E111-E114
4. Usami S, Yamamoto M, Okazaki M. Intraoperative ultrasound-assisted repair of zone III ﬂexor tendon
rupture with a minimal incision The Journal of Hand Surgery. 2016; 41(9); 1003-1004
5. Usami S, Kodaira S, Okazaki M. Primary On-top Plasty for Treatment of Short-type Postaxial Polydactylyof the Foot Annals of Plastic Surgery. 2016; 77(2); 223-225
6. Usami S, Ohkubo A, Okazaki M. Vascularized Nerve Bypass Graft: A Case Report of an Additional
Treatment for Poor Sensory Recovery PRS Global Open. 2016; 4(4); e686-e689
7. Yano T, Okazaki M, Tanaka K, Iida H. The Flap Sandwich Technique for a Safe and Aesthetic Skull Base
Reconstruction Annals of Plastic Surgery. 2016; 76(2); 193-197
8. Yano T, Okazaki M, Tanaka K, Tsunoda A, Aoyagi M, Kishimoto S. Use of Intraoperative Fluorescent
Indocyanine Green Angiography for Real-Time Vascular Evaluation of Pericranial Flaps Annals of Plastic
Surgery. 2016; 76(2); 198-204
9. Yano T, Okazaki M, Tanaka K, Tsunoda A, Aoyagi M, Kishimoto S. Feasibility and Advantage of a
Muscle-Sparing Laterally Based Pericranial Flap The Journal of Craniofacial Surgery. 2016; 27(3); 552557
10. Yano T, Suesada N, Usami S. Esthetic Craniofacial Bony and Skull Base Reconstruction Using Flap
Wrapping Technique The Journal of Craniofacial Surgery. 2016; 27(5); 1234-1238
11. Sakisaka M, Kurita M, Okazaki M, Kagaya Y, Takushima A, Harii K. Drug-Induced Atrial Fibrillation
Aggravates the Results of Flap Surgery in a Rat Model Annals of Plastic Surgery. 2016; 76; 244-248
12. Namiki T, Miura K, Ueno M, Tanaka K, Yokozeki H. Case of basal cell carcinoma with myoepithelial
diﬀerentiation: Its characteristic clinical and histopathological features The Journal of Dermatology. 2016;
43(9); 1109-1110[Conference Activities & Talks]
1. Mori H, Uemura N, Okazaki M. Orbital myectomy using brow lift approach in blepharospasm. The 13th
Japan-Korea Congress of Plastic and Reconstructive Surgery 2016.05.17 Kanazawa
2. Usami S, Kawahara S, Shibahara H, Abe K, Yamaguchi T, Minamikawa Y. Surface replacement arthroplasty using Self Locking Finger Joint implant for the painful proximal interphalangeal joint osteoarthritis.
The 13th Japan-Korea Congress of Plastic and Reconstructive Surgery 2016.05.17 Kanazawa
3. Homma T, Okazaki M, Tanaka K, Uemura N. Simultaneous surgical treatment for smile dysfunction andlagophthalmos involving a dual latissimus dorsi neuromuscular ﬂap transfer combined with fascia lata
grafting. The 3rd meeting of Asian Paciﬁc Federation of Societies 2016.06.04 Beijin
4. Tanaka K, Suesada N, Yano T, Okazaki M. Indication of Reconstructive Surgery for Middle Skull Base
Defects. The 7th International Congress of the World Federation of Skull Base Societies 2016.06.15 Osaka
5. Imai K, Tanaka K, Suesada N, Homma T, Okazaki M, Kishimoto S. One-stage reconstruction of theextensive defects involving middle skull base and orbital ﬂoor with the combination of temporalis muscle
ﬂap and free ALT ﬂap. The 7th International Congress of the World Federation of Skull Base Societies
2016.06.17 Osaka― 63 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

6. Mori H, Mihara R, Okazaki M. Nipple reconstruction with dorsal skin provides better projection thanreconstruction with abdominal or breast skin with cartilage grafting. Plastic Surgery The Meeting 20162016.09.24 Los Angels
7. Tanaka K, Igari K, Kishino M, Usami S, Homma T, Toyofuku T, Inoue Y, Okazaki M. The possibility offree tissue transfer to be the nutrient ﬂap for critical ischemic foot based on the postoperative angiographyﬁndings. World Union of Wound Healing Societies 2016.09.25 Florence
8. Haraguchi M, Tanaka K, Sumita YI, Okazaki M, Taniguchi H. Prosthetic rehabilitation for a patient withextensive vascular malformation in the head and neck region. The 63rd Annual Meeting of the American
Academy of Maxillofacial Prosthetics 2016.10.01 San Diego― 64 ―

Maxillofacial
Maxillofacial andand Neck
Neck Reconstruction
Reconstruction

Head and Neck Surgery

Professor: Takahiro Asagkage
Project professor: Seiji Kishimoto
Junior associate professor: Takuro Sumi, Yosuke Ariizumi
Assistant professor: Yusuke Kiyokawa, Fuminori Nomura
Senior Resident: Fuminori Nomura, Akihisa Tasaki, Ryuhei Okada
Student: Hirofumi Fukushima, Takao Tokumaru, Masaharu Kishikawa(1)OutlineOur department is responsible for clinical management, education and research in the ﬁeld of head and necksurgery. Clinically, the Department of Head and Neck Surgery manages the tumor of extensive area of head andneck, except brain, eye and vertebra. Surgical and medical treatment of the head and neck tumors are mainly
employed in our department.

(2)

Research

Surgical approaches to the skull base and deep area of the face.
Surgical anatomy of the skull base.
Establishment of the standard neck dissection.
Treatment of pediatric head and neck tumors.
Chemoradiotherapy for head and neck cancers.
Clinical application of navigation system and 3D entity model surgery for skull base surgery.
Diagnosis and treatment for superﬁcial squamous cell carcinoma of head and neck region.
Human papilloma virus infection and head and neck cancer.
Polymorphisms in alcohol metabolism genes and Head and Neck Cancers.

(3)

Education

Education: Undergraduate Course
In the classes at the 3th grade of medical school, head and neck oncology are systematically lectured. Clinicalpractice is experienced at the 4th to 6th grade in the out-patient clinic, the ward, and operating theater.
Education :Graduate Course
Education and researches at the graduate school are focused on (1)surgery of the head and neck tumor,(2)clinical management of the patients with head and neck tumor, and (3)clinical anatomy of head and neckregion.

― 65 ―

Maxillofacial andReconstruction
Maxillofacial
and Neck
Neck Reconstruction(4)

Publications[Original Articles]
1. .Hirofumi Fukushima,Takeharu Kanazawa,Kazuyoshi Kawabata,et al. Indwelling Voice Prosthesis insertion after Total Pharyngolaryngectomy with Free Jejunal Reconstruction. Laryngoscope Investigative
Otolaryngology. 2016;
2. Kondo T, Sato Y, Tanaka H,Fukushima H,et al. Two Cases of Ectopic Hamartomatous Thymoma Masquerading as Sarcoma. Case Rep Otolaryngol.. 2016;
3. K Ichikura, A Yamashita, S Kishimoto, et al. Persistence of psychological distress and correlated factors
amongpatients with head and neck cancer Palliative and Supportive Care. 2016.02; 14(1); 42-51
4. Ichikura Kanako, Yamashita Aya, Sugimoto Taro, Kishimoto Seiji, Matsushima Eisuke. Persistence of
psychological distress and correlated factors among patients with head and neck cancer. Palliat Support
Care. 2016.02; 14(1); 42-51
5. Nakano K, Sato Y, Toshiyasu T, Fukushima H,et al. Predictive factors of head and neck squamous cell
carcinoma patient tolerance to high-dose cisplatin in concurrent chemoradiotherapy. Mol Clin Oncol.
2016.02; 4(2); 303-309
6. T Yano,M Okazaki,K Tanaka.A Tsunoda,M Aoyagi,S Kishimoto . Use of Intraoperative Fluorescent
Indocyanine Green Angiography for Real-Time Vascular Evaluation of Pericranial Flaps Annals of plastic
surgery . 2016.02; 76(2); 198-204
7. T Yano, M Okazaki, K Tanaka, S Kishimoto:etal. Feasibility and Advantage of a Muscle-Sparing Laterally
Based Pericranial Flap. Journal of Craniofacial Surgery. 2016.05; 27(3); 552-557
8. Nakano K, Sato Y, Sasaki T, Shimbashi W, Fukushima H,et al. Combination chemotherapy of carboplatin
and paclitaxel for advanced/metastatic salivary gland carcinoma patients: diﬀerences in responses by
diﬀerent pathological diagnoses Acta Otolaryngol. . 2016.09; 136(9); 948-951
9. K. Nakagawa, R Yoshimura, Skishimoto,etc. Tongue reconstruction with minimal donor site morbidity
using a deep inferior epigastric perforator (DIEP) free ﬂap in a 6-year-old girl Microsurgery . 33(6);487-490[Conference Activities & Talks]
1. M Kishimoto, T Nomura, S Kishimoto 、et al. A case report of advanced infantile ﬁbromatosis involving
masseter muscle and mandible. . 16th Japan-Korea joint meeting of ORL-HNS, 2016.03.29 東京
2. Sugimoto T.. ELPS for the patients with laryngo-pharyngeal cancer. A Collaborative Laboratory Investigation by Stanford Head and Neck Surgery and Intuitive Surgical, Inc.. Transoral Endoscopic Head &Neck Surgery for the Hypopharynx and Cervical Esophagus, 2016.05.24 Calfornia ,USA
3. Sugimoto T. How I do it? ELPS using a small diameter upper GI endoscope. 2016 International Symposium of Transoral Head & Neck Surgery 2016.05.28 Seoul、Korea
4. Sugimoto T. How to Maintain Quality and Standards of Transoral Head and Neck Surgery Oncological
Outcomes in transoral head and neck surgery including ELPS. 2016 International Symposium of Transoral
Head & Neck Surgery 2016.05.28 Seoul、Korea
5. Okada R, Sugimoto T, Sumi, et al. The successful performance of two transoral partial hypopharyngectomy operations after chemotherapy for local metachronous recurrence following chemoradiotherapy foradvanced hypopharyngeal cancer: A case report. ANHS 9th international conference on head and neckcancer. 2016.06 washington
6. Asakage T. Chairman. Breakfast Seminar 2-3 Ultimate Management of Orbital and Frontal Sinus Tumor..
7th International congress of the world federation of skull base societies 2016.06.14 Osaka
7. Asakage T. Surgical treatment of frontal sinus carcinoma. Breakfast Seminar 2-3 Ultimate Managementof Orbital and Frontal Sinus Tumor. 7th International congress of the world federation of skull basesocieties. 2016.06.15 Osaka― 66 ―

Maxillofacial
Maxillofacial andand Neck
Neck Reconstruction
Reconstruction

S Kishimoto,et al. One-stage reconstruction of the extensive defects
8. K Imai, K Tanaka, N Suesada,involving middle skull base and orbital ﬂoor with the combination of temporalis muscle ﬂap and free ALT
ﬂap.. 7th International congress of the world federation of skull base societies 2016.06.15 osaka
9. Y Kawano、M Aoyagi、T Asakage, S Kishimoto. Multidisciplinary surgical approach for advanced juvenile
nasopharyngeal angioﬁbroma with skull base and cavernous sinus extension. . 7th International Congressof the World Federation of Skull Base Societies 2016.06.15 Osaka
10. T Sugawara, M Aoyagi, S Kishimoto, et al. Extended orbital exenteration for sinonasal malignancy
w/ orbital apex extension. 7th International Congress of the World Federation of Skull Base Societies
2016.06.15 Osaka
11. S Kishimoto, A Tsunoda, et al. Craniofacial surgery for advanced juvenile nasopharyngeal angioﬁbromaswith intracranial extension. 7th International Congress of the World Federation of Skull Base Societies
2016.06.15 Osaka12. S Kishimoto, A Tsunoda,et al. Surgical treatment of pediatric sarcomas in the skull base. . 7th International Congress of the World Federation of Skull Base Societies 2016.06.15 Osaka
13. Ryuhei Okada, Taro Sugimoto, Takuro Sumi, Taro Fujikawa, Yusuke Kiyokawa, Fuminori Nomura, Akihisa Tasaki, Tomoaki Asamori, Seiji Kishimoto, Takahiro Asakage. Chemotherapy and followed endoscopic laryngo-pharyngeal surgery (ELPS) is useful for recurrence of pharyngeal carcinoma: a case report.
AHNS 9TH International Conference on Head and Neck Cancer 2016.07.16 Washington
14. S Kishimoto. Surgical treatment for pediatric head and neck sarcomas with skull base invasion . 2016
Annual Meeting of Taiwan Head and Neck Society in conjunction with International Head and Neck
Cancer Conference & Cross-Straits HNS Forum( 2016.10.15 Taipei
15. S Kishimoto. Lecture "Craniofacial approach to the skull base", . Morning Lecture in Tainan Cheng Gung
University 2016.10.17 Tainan
16. Asakage T. Year in review from Japan. International session 16 head and neck cancer . 54th Japan
Society Clinical Oncology annual meeting 2016.10.20 Yokohama
17. R Okada, T Sugimoto, T Sumi, T Fujikawa, S Kishimoto、et al. The successful performance of two
transoral partial hypopharyngectomy operations after chemotherapy for local metachronous recurrence
following chemoradiotherapy for advanced hypopharyngeal cancer A case report.. AHNS 9th International Conference on Head and Neck Cancer Seattle USA― 67 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

Radiation Therapeutics and Oncology

Professor Ryoichi Yoshimura
Lecturers Kazuma Toda
Research Associates Keiko Nakagawa,Mio Kojima
HospitalStaﬀmembers HirohumiKuwabara(∼Mar),TakuyaNagano(Apr∼)
ResJdent TakuyaNagano(∼Mar)(1)

OutlineAt the Department of Radiation Therapeutics and Oncology, clinical services, research, and education related
to radiotherapy for all cancers are undertaken.
(2)

Research

Mainly clinical research related to radiotherapy is performed.
(3)

Education

Lectures are given to medical students or graduate students, and clinical clerkship is organized.
After the students enter our department, comprehensive training is provided at both our department and theDepartment of Diagnostic Radiology and Nuclear Medicine for 3 years, since the certiﬁed radiologist exam iscommon.
Our department holds a radiation oncology seminar for students and residents with the Department ofRadiation Oncology of Juntendo University and Showa University.
Lectures for medical students or graduate students, and clinical clerkship are performed.

(4)

Lectures & Courses

Our department teaches students and residents about cancer therapy from the radiation oncologist’s perspective.
Every student/resident of this department aims to be a certiﬁed radiation oncologist.

(5)

Clinical Services & Other Works

All the staﬀ members are engaged in performing external beam radiation therapy or high-dose-rate or lowdose-rate brachytherapy in the hospital.
A total of 596 patients, including 132 head and neck cancer patients, 126 urological cancer patients, 90 breast
cancer patients, 64 lung cancer patients, and 53 esophageal cancer patients, were treated by external beam
radiotherapy at our hospital in 2015. Moreover, high-dose-rate brachytherapy was performed in 51 patientswith uterine cancer, and low-dose-rate brachytherapy in 58 patients with oral cancer,and in 19 patients withprostate cancer.

― 68 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction(6)

Clinical Performances

Our department specializes in low-dose-rate brachytherapy for oral cancer patients.
(7)

Publications[Original Articles]
1. Taito Asahina, Atsushi Kaida, Tatsuaki Goto, Ryo-Ichi Yoshimura, Keisuke Sasai, Masahiko Miura.
Temporo-spatial cell-cycle kinetics in HeLa cells irradiated by Ir-192 high dose-rate remote afterloading
system (HDR-RALS). Radiat Oncol. 2016.07; 11; 99
2. Murakami N, Kato S, Nakano T, Yamanaka T, Sakurai H, Yoshimura R, Hiratsuka J, Kuroda Y, Yoshioka
K, Itami J. A phase I/II clinical trial for the hybrid of intracavitary and interstitial brachytherapy for
locally advanced cervical cancer. Bio Med Central Cancer. 2016.08; 16(640);[Conference Activities & Talks]
1. Ryoichi Yoshimura. Multi-institutional joint research of brachytherapy Head and Neck/Breast. JapaneseGroup of Brachytherapy/Japanese Society for Therapeutic Radiology and Oncology 2016.05.28 Osaka
2. Hirofumi Kuwabara, Akira Toriihara, Keiko Nakagawa, Kazuma Toda, Ukihide Tateishi, Ryoichi Yoshimura.
Utility of metabolic tumor burden calculated from dual-time-point FDG positron emission tomography/computed tomography to evaluate prognostic value of oropharyngeal or hypopharyngeal cancer..
ASTRO 2016 Annual Meeting 2016.09.25
3. Ryoichi Yoshimura. Brachytherapy techniques: intracavitary implants. The 4th Congress of the Japan
Oncoplastic Breast Surgery Society 2016.10.06 Chiba
4. Watanabe H, Ozaki Y, Miura M, Yoshimura R, Sumi Y, Kurabayashi T. Estimation of whole bodyradiation exposure induced by oral cancer brachytherapy using Monte Carlo simulation. The 11th AsianCongress of Oral and Maxillofacial Radiology 2016.11.12 Chiang Mai, Thailand
5. Retrospective review of POEMS syndrome initially treated with radiotherapy in our institute. The 29th
annual meeting of the japanese society for radiation oncology 2016.11.27
6. Yuasa-Nakagawa K, Yoshimura R, Toda K, Kojima M, Shibuya H. The therapeutic changes inﬂuence on
the treatment results of the hypopharyngeal cancer. ESMO-ASIA 2016 2016.12.17 Singapore― 69 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

Maxillofacial Anatomy

Professor
Shunichi SHIBATA
Assistant Professor
Shun-ichi SHIKANO
Graduate Student
Masato Takahashi
Graduate Student
Maki Hasegawa
Gradiate Student Shota Kusaka
Lecturer
Rei Sato
PD Chiho Watanabe(1)OutlineMaxillofacial anatomy section is engaged in lecture and practical course of gross anatomy and dental anatomy
in undergraduate school. In graduate school, this section is engaged in morphological studies of hard tissues
such as tooth, bone and cartilage.
(2)

ResearchResearch Subjects
1) Structural features of mandibular condylar cartilage.
2) Mechanism of epithelial attachment of junctional epithelium in human gingiva.
3) Comparative histology and embryology of teeth.
4) Observation on the structural features of oral mucous
5) Anatomical names of the structures of human skeletal system.
6) Hyaluronan synthesis in tooth germ.
7) Studies on regeneration of jaw bone.
8) Structural features of dental pulp and extracellular matrix(3)

Education

In Undergraduate school
Lecture for 2nd degree students: Human structure I, II, Dental anatomy, Neuroanatomy,
Practical course for 2nd degree students: Gross Anatomy, Neuroanatomy, Dental Anatomy
Lecture and practical course for 5th degree students: Clinical craniofacial anatomy
In Graduate school
Lecture, seminar and practical course to understand the function of various oral organs in a morphological
viewpoint, and to evaluate various vital phenomenon encountered in medical practice.
(4)

Lectures & Courses

The main purpose of education in undergraduate school is to understand human structure and function fromthe viewpoints of gross anatomy. In line with this purpose, we execute lectures of systematic anatomy (osteology, myology, neurology, angiology, splanchnology) and topographic anatomy (craniofacial anatomy). To
understand three-dimensional structures of human body, we execute practical course of human gross anatomy― 70 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

after completing lectures. In the practical course, we make an eﬀort to make students understand ethics asdental students to be bright future dentist.
The main purpose of education in graduate school is to understand various vital phenomenon, which we encounter in research ﬁelds of basic and clinical sciences, from the viewpoints of morphology. In lectures, we teachvarious techniques to investigate structural features from the standpoints of light and electron microscopy, organ
and tissue culture, and molecular biology.
(5)

Publications[Original Articles]
1. Sato R, Fukuoka H, Yokohama-Tamaki T, Kaku M, Shibata S. Immunohistochemical localization oftenascin-C in rat periodontal ligament with references to alveolar bone remodeling Anatomical Science
International. 2016.02; 91(2); 196-206
2. Hirano-Kawamoto A, Honkura Y, Shibata S, Abe S, Murakami G, Katori Y. Cricoarytenoid articulationin elderly Japanese with special reference to morphology of the synovial and capsular tissues Annals of
Otology, Rhinology, and Laryngology. 2016.03; 125(3); 219-227
3. Hayashi K, Takahashi T, Ikegawa M, Horie M, Oyaizu T, Enomoto M, Shibata S, Yagishita K, Ueno T.
The facilitatory eﬀects of hyperbaric oxygen treatment on membrane bone wound healing in a rat calvarial
defect model. Undersea Hyperbaric Medicine. 2016.03; 43(2); 135-142
4. Cho KH, Jin ZW, Abe H, Shibata S, Murakami G, Rodríguez-Vázquez JF. Neural-dural transition at thethoracic and lumbar spinal nerve roots: a histological study of human late-stage fetuses BioMed ResearchInternational. 2016.05; 2016(8163519);
5. Morita T, Fujikawa K, Baba O, Shibata S. An in situ hybridization study of Hyaluronan synthase (Has)mRNA in developing mouse molar and incisor tooth germs Gene Expression Patterns. 2016.05; 21(1);28-40
6. Noda M, Aoki A, Mizutani K, Lin T, Komaki M, Shibata S, Izumi Y. High-frequency pulsed low-level
diode laser therapy accelerates wound healing of tooth extraction socket: An in vivo study. Lasers inSurgery and Medicine. 2016.07; 48(10); 955-964
7. Jin ZW, Abe H, Jin Y, Shibata S, Murakami G, Rodríguez-Vázquez JF. Positional changes in tendon
insertions from bone to fascia: development of the pes anserinus and semimembranosus muscle insertionin human foetuses Folia Morphologica. 2016.11; 75(4); 503-511[Conference Activities & Talks]
1. Shibata S. Expression of Hyaluronan Synthase (Has) in Developing Mouse Molar and Incisor Tooth Germ.
2015 Frontier meeting in Hong Kong 2016.02.12 The University of Hong Kong, Hong Kong , China.
2. Ito Y, Takahashi Y, Hikita R, Watanabe C, Akiyama S, Higashihori N, Moriyama K. Long-term changes
in clinical crown height after surgical orthodontic treatment for mandibular prognathism. The 10th Asia
Paciﬁc orthodontic Conference and 11th Annual Meeting of the Indonesian Association of Orthodontists
2016.09.03 Bali,Indonesia
3. Masahiro Noda, Akira Aoki, Koji Mizutani, Taichen Lin, Motohiro Komaki, Shunichi Shibata, Yuichi
Izumi. Eﬀect of low level laser irradiation on early stage of wound healing of tooth extraction socket. The
23rd General Meeting of the Japanese Association for Dental Science 2016.10 Fukuoka― 71 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

Cognitive Neurobiology

Professor Masato Taira
Junior Associate Professor Hisayuki Ojima
Assistant Professor Narumi Katsuyama
Assistant Professor Nobuo Usui
Research fellow Rui Watanabe
Graduate Student Eriko Kikuchi(Tachi)
Yuko Imai
Kono Youko
Maki Okada
Hiroaki Tanaka
Adjunct instructor Saneyuki Mizutani(1)

OutlineHigher brain functions have been studied in this laboratory. To explore the individual functions listed below,
functional MRI, psychological or psychophysical approach, and traditional electrophysiological recordings together with animal training are applied to animal models such as non-human primates and rodents as well as tohuman. Our goal is to clarify how perceptional mechanisms underlying higher brain functions are described by
the neuronal activity pattern and how cortical wide connectivity is interacted while brain is judging, decision
making, extracting biological meanings of sounds including languages.
(2)

Research

1. Neural Mechanisms of control of motor behavior.
Research is aimed at understanding the brain mechanisms of execution and control of the motion and behaviorof animals and human.
2. Neuronal mechanisms for perception and cognition.
Research is aimed at understanding the brain mechanisms of perception and cognition of objects through visionand tactile senses of animals and human.
3. Processing of natural sounds in auditory cortex
Research is aimed at understanding the brain mechanisms of hearing and vocalization of animals.

(3)

Education

1. Lectures of unit "Functions of Nervous Systems I (Introduction to Neurophysiology, Motor Functions)
Basic knowledge of neurophysiology will be lectured as an introduction together with the motor functions.
2. Lectures of unit "Functions of Nervous Systems II (Perception, Emotion, Instinct, Sleep, Higher functions) .
A series of lectures will be taught on functions of the sensation, perception, and motion as well as the neuralmechanisms of higher brain functions.
3. Lectures of unit "Homeostatic Functions for Life Support".
Lectures will be taught on the structure of the autonomic nervous system and its regulatory mechanisms in thecirculation, respiration, digestion/absorption, humor/body temperature, metabolism, excretion, and internal
secretion/reproduction.

― 72 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

4. Lectures of unit "Oral Physiology"
Lectures will be taught on the structure and function of various somatosensory organs in the oral cavity. Neural
regulations of mastication and deglutition as well as the secretion mechanism of saliva will also be learned.
5. Unit of Practice in Physiological Functions
The purpose of the practice is to learn about the physiological mechanisms underlying the normal functions ofhuman body through experiments. The goal is to master the basic experimental procedures, and to experience
how to capture and analyze the data in order to draw conclusions.

(4)

Lectures & Courses

Students are expected to be voluntarily involved in research activity. Brain is an extremely complicated organ
in terms of its morphology and functions. Learning of textbook knowledge is mandatory and is followed bycapturing up-to-date scientiﬁc information through reading published articles. Once the overview of the ﬁeld
in which graduates/undergraduate students are interested in is completed, they will start to be engaged inon-going research topics under the tutelage of laboratory staﬀ. Through this process, students are encouraged
to associate their own research interests with the on-going research topics and will learn practically how data
can be captured, rearranged and analyzed through real experiments.

(5)

Publications[Original Articles]
1. Hisayuki Ojima and Junsei Horikawa. Recognition of modiﬁed conditioning sounds by competitively
trained guinea pigs Frontiers in Behavioral Neuroscience. 2016.01; 26(9); 373
2. Yasuhiro Mochizuki, Tomokatsu Onaga, Hideaki Shimazaki, Takeaki Shimokawa, Yasuhiro Tsubo, Rie
Kimura, Akiko Saiki, Yutaka Sakai, Yoshikazu Isomura, Shigeyoshi Fujisawa, Ken-Ichi Shibata, Daichi
Hirai, Takahiro Furuta, Takeshi Kaneko, Susumu Takahashi, Tomoaki Nakazono, Seiya Ishino, Yoshio
Sakurai, Takashi Kitsukawa, Jong Won Lee, Hyunjung Lee, Min Whan Jung, Cecilia Babul, Pedro E
Maldonado, Kazutaka Takahashi, Fritzie I Arce-McShane, Callum F Ross, Barry J Sessle, Nicholas G
Hatsopoulos, Thomas Brochier, Alexa Riehle, Paul Chorley, Sonja Grün, Hisao Nishijo, Satoe IchiharaTakeda, Shintaro Funahashi, Keisetsu Shima, Hajime Mushiake, Yukako Yamane, Hiroshi Tamura, Ichiro
Fujita, Naoko Inaba, Kenji Kawano, Sergei Kurkin, Kikuro Fukushima, Kiyoshi Kurata, Masato Taira,Ken-Ichiro Tsutsui, Tadashi Ogawa, Hidehiko Komatsu, Kowa Koida, Keisuke Toyama, Barry J Richmond,
Shigeru Shinomoto. Similarity in Neuronal Firing Regimes across Mammalian Species. J. Neurosci..
2016.05; 36(21); 5736-5747
3. Rui Watanabe, Takahiro Higuchi. Behavioral advantages of the ﬁrst-person perspective model for imitation. Frontiers in Psychology. 2016.05; 7(701);
4. Chiharu Matsuda, Toshio Shimizu, Yuki Nakayama, Michiko Haraguchi, Yoko Mochizuki, Chiyoko Hakuta,
Masato Taira, Takaya Numayama, Masanobu Kinoshita. Analysis of resting salivation rate in patientswith amyotrophic lateral sclerosis using tracheostomy invasive ventilation. Rinsho Shinkeigaku. 2016.07;
56(7); 465-471
5. T Shinozaki, Y Imamura, R Kohashi, K Dezawa, Y Nakaya, Y Sato, K Watanabe, Y Morimoto, T
Shizukuishi, O Abe, T Haji, K Tabei, M Taira. Spatial and Temporal Brain Responses to Noxious Heat
Thermal Stimuli in Burning Mouth Syndrome. J. Dent. Res.. 2016.09; 95(10); 1138-1146
6. Narumi Katsuyama , Nobuo Usui, Masato Taira. Activation of the Human MT Complex by Motion in
Depth Induced by a Moving Cast Shadow PLoS One. 2016.09; 11(9); e0162555
7. Chiharu Matsuda, Toshio Shimizu, Yuki Nakayama, Michiko Haraguchi, Chiyoko Hakuta, Yumi Itagaki,
Akiko Ogura, Kanako Murata, Masato Taira, Takaya Numayama, Masanobu Kinoshita. Macroglossia in
advanced amyotrophic lateral sclerosis. Muscle Nerve. 2016.09; 54(3); 386-390― 73 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction[Books etc]
1. Fukushima M, Ojima H. Information Processing in the Auditory Ventral Stream Brain and Nerve 68(11);1371-1378. 2016.11[Misc]
1. Makoto Fukushima, Hisayuki Ojima. [ Information Processing in the Auditory Ventral Stream] . Brain
Nerve. 2016.11; 68(11); 1371-1378[Conference Activities & Talks]
1. Hayashi K, Ojima H, Horikawa J . Changes in temporal pattern of AI activity after interval discriminationof repetitive sounds in guinea pigs. 39th Annual Midwinter Meeting of ARO 2016.02.20 San Diego, CA,
USA
2. Eriko Kikuchi,Hisayuki Ojima,Akiko Yamashita,Narumi Katsuyama,Masato Taira. Eﬀects of stimulus
intensity on the induced jaw movement patterns in guinea pigs. 8th CBIR Inspive Symposium 2016.02.20
3. Ayako Kubota, Yoko Kono, Hiroko Imura, Hiroji Shimomura, Kiyoko Kanamori, Yasuko Kawakami, Shiro
Mataki, Kumiko Sugimoto. Oral health status and behavior of Japanese university students. International
Symposium on Dental Hygiene 2016 2016.06 Basel, Switzerland
4. Ikeda A, Miyamoto J, Usui N, Taira M, Moriyama K.. Eﬀect of mastication on satiety using an attentional bias task.. 94th General Session & Exhibition of the International Association for Dental Research
2016.06.24
5. Akitsu Ikeda, Jun Miyamoto, Nobuo Usui, Masato Taira, Keiji Moriyama. Eﬀect of Mastication on Satiety
Using an Attentional Bias Task. 94th General Session&Exhibition of the IADR 2016.06.24
6. Sawada A, Usui N, Shimazaki K, Taira M, Ono T.. Neural mechanisms underlying time-dependentchanges in orthodontic pain.. 94th General Session & Exhibition of the International Association for
Dental Research 2016.06.25
7. Alisa Sawada, Nobuo Usui, Kazuo Shimazaki, Masato Taira, Takashi Ono. Neural Mechanisms Underlying
Time-dependent Changes in Orthodontic Pain. 2016.06.25
8. Rui Watanabe et al.. Eﬀects of perspective and motion of body parts on imitation behavior: investigationfrom neural aspects by fMRI. 10TH FENS FORUM OF NEUROSCIENCE 2016.07.04
9. Hisayuki Ojima,Koki Hayashi,Masato Taira,Junsei Horikawa. Changes in Temporal Activation Pattern of
the Guinea Pig’s AI after Interval Discrimination. The 39th Annual Meeting of the Japanese Neuroscience
Society 2016.07.21 Yokohama, Japan
10. Fujiwara J, Tsutsui K-I, Taira M, Ugawa Y, Eifuku S. Neural mechanism of elf-consistency in social
behavior . The 39th Annual Meeting of the Japan Neuroscience Society 2016.07.21
11. Eﬀects of stimulus tensities on the induced jaw movement patterns in guinea pigs. 2016.07.21
12. Eriko Kikuchi,Hisayuki Ojima,Akiko Yamashita,Narumi Katsuyama,Masato Taira. Eﬀects of stimulus
intensities on the induced jaw movement patterns in guinea pigs. The 39th Annual Meeting of the Japan
Neuroscience Society 2016.07.22
13. J. Fujiwara. P.N. Tobler. K.I. Tsutsui. M. Taira. Y. Ugawa. S. Eifuku. Neural mechanisms underlying
self-consistency in social behavior. 46th Annual Meeting of the Society for Neuroscience 2016.11.15― 74 ―

Maxillofacial
Maxillofacial andand Neck
Neck Reconstruction
Reconstruction

Molecular Craniofacial Embryology

Staﬀs and Students
Professor
Associate Professor
Tenure Track Assistant Professor
Part-time lecturers
Visiting Researcher
Graduate Students(1)

Sachiko Iseki
Masa-Aki Ikeda
Masaki Takechi
Hirofumi Doi, Shumpei Yamada,Shigeru Okuhara
Yoichiro Ninomiya
Toshiko Furutera, Mya Nandar,
Charoenlarp Ponkawee,
Norisuke Yokoyama,
Erika Kubota (Pedodontics),
Tian Xiaohui (Oral Implantology
and Regenerative Dental
Medicine),
Rajendran Arun Kumar,
Takahiko Yamada (Maxillofacial Surgery), Vou Hoang Tri (Oct-)

Research

1) Molecular mechanisms of mammalian craniofacial development
2) Application of developmental mechanisms to regenerative medicine
3) Identiﬁcation of tissue stem cells in craniofacial region and molecular mechanism of their stemness
4) Regulation of gene expression in cell growth and stress response
5) Nuclear architecture and function in regulating gene expression(2)

Publications[Original Articles]
1. Otsuki Y., Ueda K., Satoh C., Maekawa R., Yoshiura K., Iseki S.. Intermediate phenotype between
ADULT syndrome and EEC syndrome caused by R243Q mutation in TP63 Plastic and Reconstructive
Surgery-Global Open. 2016.12; 4(12); e1185[Misc]
1. Takechi, M., Kitazawa, T., Hirasawa, T., Hirai, T., Iseki, S., Kurihara, H., Kuratani, S.. Developmental
mechanisms of the tympanic membrane in mammals and non-mammalian amniotes. Congenit Anom
(Kyoto). 2016.01; 56(1); 12-17[Conference Activities & Talks]
1. Ikeda MA. Novel Candidates of Therapeutic Targets for Head and Neck Squamous Cell Carcinomas
Deﬁned by Recent Comprehensive Mutation Analysis . The 17th Scientiﬁc and Refresher Course in
Dentistry (KPPIKG 2016) 2016.02.27 Jakarta, Indonesia― 75 ―

Maxillofacial andReconstruction
Maxillofacial
and Neck
Neck Reconstruction

2. Yuki Otsuki, Koichi Ueda, Chisei Sato, Koichiro Yoshiura, Sachiko Iseki. A intermediate phenotype
between ADULT syndrome and EE syndrome caused by R243Q mutation in TP63.. The 13th Japan
Korea Congress of Plastic and Reconstructive Surgery 2016.05.15― 76 ―

Maxillofacial
Maxillofacial andand Neck
Neck Reconstruction
Reconstruction

Cellular Physiological Chemistry

Associate Professor Ken-ichi Nakahama
Assistant Professor Yasuhiro Yoshimatsu
Junior Associate Professor Hiroshi Fujita, Yasuki Ishizaki, Masao Saito
Research Student: Mizuki Nagata,:Syun Nishihara:Hiroki Kuwahara:Syuhei Fukuda:Asuka Okito(1)

OutlineIn our Lab, we study the role of cell-communication in bone remodeling, cancer and vascular calciﬁcation using
various techniques, for example, cell culture, molecular biology and mutant mice.

(2)

ResearchResearch Subjects
1, Cell-cell communication and cell functions
2, Bone remodeling and cell communications
3, Cancer and cell communications
4, Mechanism of vascular calciﬁcation(3)

Education

For undergraduate students. We have some class in biological chemistry for the second grader.
For graduate students. These students can choose the one of themes in our lab. They have to attend meetingsand seminars in our Lab.

(4)

Lectures & Courses

Undergraduate students should understand basic biochemistry and physiology under healthy/diseased conditions.
Graduate students are expected to solve the problems by themselves. However, appropriate suggestions will begiven by at least three supervisors whenever you want.

― 77 ―

Maxillofacial andReconstruction
Maxillofacial
and Neck
Neck Reconstruction(5)

Publications[Original Articles]
1. Tsuyoshi Matsuura, Shizuko Ichinose, Masako Akiyama, Yuki Kasahara, Noriko Tachikawa, Ken-Ichi
Nakahama. Involvement of CX3CL1 in the Migration of Osteoclast Precursors Across Osteoblast Layer
Stimulated by Interleukin-1ß. J. Cell. Physiol.. 2016.08;[Misc]
1. Yasuhiro Yoshimatsu, Hideki Miyazaki, Tetsuro Watabe. Roles of signaling and transcriptional networks
in pathological lymphangiogenesis. Adv. Drug Deliv. Rev.. 2016.04; 99(Pt B); 161-171
2. Yasuhiro Yoshimatsu. Roles of Transcription Factors in the Formation of Lymphatic Vessels Inﬂammation
and Immunity. 2016.08; 24(5); 372-378[Conference Activities & Talks]
1. Ken-ichi Nakahama, Tsuyoshi Matuura, Takako Tachikawa. Involvement of CX3CL1 in the migration of
osteoclast precursors across osteoblast layer stimulated by interleukin-1beta.. The 2nd annual meeting ofJapanese Society of Osteoimmunology 2016.07.07
2. Asuka Okito, Masako Akiyama, Jun Hosomichi, Takashi Ono, Ken-ichi Nakahama. Transcriptional regulation of osteopontin in osteoblasts.. The 2nd annual meeting of Japanese Society of Osteoimmunology
2016.07.07
3. Masahiro Shinohara, Ken-ichi Nakahama, Hiroshi Asahara. Regulation of osteoblastic bone formation byclass IA PI3K in osteocytes. The 2nd annual meeting of Japanese Society of Osteoimmunology 2016.07.07
4. Kazuki Takahashi, Katarzyna Anna Inoue,Yasuhiro Yoshimatsu. Hironori Harada and Tetsuro Watabe.
Role of TGF-β in the invasiveness of oral cancer cell lines. The 88th Annual Meeting of Molecular Biology
Society of Japan 2016.12 Yokohama
5. Yasuhiro Yoshimatsu. Roles of signaling and transcriptional networks in the formation and maintenanceof lymphatic vasculature. The 24th Annual Meeting of the Japanese Vascular Biology and Medicine
Organization/ The 14th Korea-Japan Joint Symposium on Vascular Biology 2016.12.10 Nagasaki― 78 ―

Maxillofacial
Maxillofacial andand Neck
Neck Reconstruction
Reconstruction

Biodesign

Professor Kazuo TAKAKUDA
Assistant Professor Hisashi DOI
Mari YUASA
Research Assistants Shukan OKANO
Hiroyuki MASUNO
Noriko NAKAISHI
Graduate Students (Doctoral coarse)
Hiroki IKEDA, Hiroyuki KUSABA,
Hisaya NOMATA, Eiko MARUKAWA(1)

Outline

Biodesign division started in 1951 at establishment of the dental materials research institute as one of theconstituting department. Since then, the division changed its name from the department of machinery, thedepartment of precision machinery, the division of mechanics, and the division of biodesign as the institutecontinued reorganization till the present form of the Institute of Biomaterials and Bioengineering. Although
our research work was originally concentrated to cutting tool for dentistry, we are now investigating mechanics of living tissues and biomaterials, biomechanical compatibility of tissues and artiﬁcial materials, and thedevelopment of artiﬁcial organs bearing the mechanical functions in living bodies.
(2)

Education

Basic-Clinical Borderless Education(3)

Publications[Original Articles]
1. Wei Wang, Soichiro Itoh, Kazuo Takakuda. Comparative study of the eﬃcacy of decellularization treatment of allogenic and xenogeneic nerves as nerve conduits. J Biomed Mater Res A. 2016.02; 104(2);445-454
2. Yagihara A, Kawasaki R, Mita A, Takakuda K.. Impact of Dynamic and Static Load on Bone Around
Implants: An Experimental Study in a Rat Model Int J Oral Maxillofac Implants. 2016.05; 31(3); 49-56
3. Kusaba H, Terada-Nakaishi M, Wang W, Itoh S, Nozaki K, Nagai A, Ichinose S, Takakuda K.. Comparisonof nerve regenerative eﬃcacy between decellularized nerve graft and nonwoven chitosan conduit Biomed
Mater Eng. 2016.05; 27(1); 75-85
4. Suzuki R, Takakuda K.. Wound healing eﬃcacy of a 660-nm diode laser in a rat incisional wound model
Lasers Med Sci.. 2016.08; 31(8); 1683-1689― 79 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

Maxillofacial Surgery

Junior Associate Professor: Satoshi YAMAGUCHI, Narikazu UZAWA
Assistant Professor: Hiroyuki YOSHITAKE, Yasuyuki MICHI, Kazuto KUROHARA, Kouichi NAKAKUKI,
Yoshio OHYAMA(from April)Keiichi MORITA
Hospital Staﬀ: Itaru SONODA, Kunihiro MYO, Nobuyoshi TOMOMATSU, Chie AKATSU, Tomomi SAKUMA,
Ryousuke NAGAOKA, Yoshinori INABA, Jun SUMINO, Chika MIURA,(from April)Yukihiko HASHIDA, Masahiko TERAUCHI, Machiko KOSUGIGraduate Student:Reiko HOSHI, Sakie KATSUMURA, Tomoki KANEMARU, Uyannga ENKHBOLD,
Syun NISHIHARA, Erina TONOUCHI, Takasuke INADA Durugu-nn BATBO-RUDO,Eri SONE,Narumi OSIBE, Keiichiro NAKAZATO,Hirosi KATO
Takahiko YAMADA, Yusun KIMU
(from April)Katsuya HYOUDOU, Daisuke YAMAMOTO
Student: (from April)Mari SHIBATA, Kentarou SUGIYAMA, Natsuko HONDA, Mayuko MARUIWA,
Tokiwa OOISHI, Mana TAKAGI
Emeritus Professor: Teruo AMAGASAClinical professor: Masashi YAMASHIRO
Part-time Lecturer: Kazuki HASEGAWA, Hitoshi MIYAOKA, Hiroyuki WAKE, Takahumi YAMADA,
Fumiaki SATO, Junichi ISHII, Eizi FUZII,Junzi KOBAYASHI,
Akiko KOBAYASHI, Yutaka SATO, Yasushi NIINAKA, Takao WATANABE
Testuo SUZUKI, Masayuki YAMANE, Takashi MISHIMAGI,
Shigehiro ABE,Miho MIZUTANI, Yasuhiro KURASAWA,
Chieko MICHIKAWA, Erika OUE, Norihiro ENDO
(1)

ResearchResearch Subjects
1) Head and Neck Surgery: Innovation of management patients with benign and malignant tumors and cysts
in oral and facial region.
2) Reconstructive Surgery: Developing method of correcting jaw, facical bone and facial soft tissue trouble left
as the result of removal of disease or previous trauma.
3) Correction of Birth Defects: Improving surgically correction of birth defects of the face and skull, including
cleft lip and palate.
4) Dentofacial Deformities and Orthognathic Surgery: Development of new surgical techniques to improving
reconstruct and realign the upper and lower jaws.
5) Temporomandibular Joint Disorders: Renewing skills in the diagnosis and treatment due to temporomandibular joint problem.
6) Oral Mucosa Disease: Creation new method with light and color for diagnosis of oral mucosa disease, including leukoplakia and cancer.

― 80 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction(2)

Lectures & Courses

Oral and maxillofacial surgery is a surgical specialty involving the diagnosis, surgical treatment and managementof defects and injures related to the function and aesthetics of the face and jaws. In order to practice the fullscope of the specialty, oral and maxillofacial surgeons are required education in dentistry, medicine and surgery
for regional requirement.
(3)

Clinical Services & Other Works

Total number of new patients was 6312 of 2015.
(4)

Clinical Performances

Clinical Services
1) Diagnosis, removing and reconstruction of jaw, oral or facial tumor or cyst.
2) Diagnosis and treatment of cleft lip and palate.
3) Treatment of jaw aligned with orthognathic surgery.
4) Therapy of temporomandibular disorder with or without temporomandibular joint surgery.
5) Diagnosis and treatment of oral mucosa disease.
6) Treatment of inﬂammation in the region jaw and facial trauma.
7) Extraction tooth including wisdom tooth.

(5)

Publications[Original Articles]
1. Okuyama K, Michi Y, Mizutani M, Yamashiro M, Kaida A, Harada K. Clinical study on mandibular
fracture after marginal resection of the mandible. Oral Surg Oral Med Oral Pathol Oral Radiol.. 2016;
2. Kato Y, Ogasawara S, Oki H, Honma R, Takagi M, Fujii Y, Nakamura T, Saidoh N, Kanno H, Umetsu M,
Kamata S, Kubo H, Yamada M, Sawa Y, Morita K, Harada H, Suzuki H, Kaneko MK. Novel Monoclonal
Antibody LpMab-17 Developed by CasMab Technology Distinguishes Human Podoplanin from Monkey
Podoplanin. Monoclon Antib Immunodiagn Immunother. . 2016; 35(2); 109-116
3. Kohmoto T, Tsuji A, Morita K, Naruto T, Masuda K, Kashimada K, Enomoto K, Morio T, Harada
H, Imoto I. A novel COL11A1 missense mutation in siblings with non-ocular Stickler syndrome. Hum
Genome Var.. 2016; 7(3); 16003
4. Khanom R, Nguyen CT, Kayamori K, Zhao X, Morita K, Miki Y, Katsube K, Yamaguchi A, Sakamoto K.
Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth. PLoS One. . 2016; 11(8); e0161163
5. Pal SK, Nguyen CT, Morita KI, Miki Y, Kayamori K, Yamaguchi A, Sakamoto K. THBS1 is induced
by TGFB1 in the cancer stroma and promotes invasion of oral squamous cell carcinoma. J Oral Pathol
Med.. 2016; 45(10); 730-739
6. Sato M, Morita K, Kabasawa Y, Harada H. Bilateral nasolabial cysts: a case report. J Med Case Rep..
2016; 10(1); 246
7. Kondo Y, Ohno T, Nishii N, Harada K, Yagita H, Azuma M. Diﬀerential contribution of three immune
checkpoint (VISTA, CTLA-4, PD-1) pathways to antitumor responses against squamous cell carcinoma.
Oral Oncology. 2016; 57; 54-60
8. Matsushita Y, Nakakuki K, Kosugi M, Kurohara K, Harada K. Does Intraoral miniplate ﬁxation have agood postoperative stability after sagittal splitting ramus osteotomy?: Comparison with intraoral bicortical screw ﬁxation. Journal of Oral and Maxillofacial Surgery. 2016.01; 74; 181-189― 81 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

9. Nakajima K, Kho DH, Yanagawa T, Harazono Y, Hogan V, Chen W, Ali-Fehmi R, Mehra R, Raz A.
Galectin-3 cleavage alters bone remodeling: Diﬀerent outcomes in breast and prostate cancer skeletal
metastasis. Cancer Res.. 2016.03; 76(6); 1391-1402
10. Morita T, Yamashiro M, Kayamori K, Mizutani M, Nakakuki K, Michi Y, Uzawa N, Izumo T, Harada K.
Primary intraosseous squamous cell carcinoma derived from a maxillary cyst: A case report and literature
review. Mol Clin Oncol.. 2016.04; 4(4); 553-558
11. Kayamori K, Katsube K, Sakamoto K, Ohyama Y, Hirai H, Yukimori A, Ohata Y, Akashi T, Saitoh M,
Harada K, Harada H, Yamaguchi A. NOTCH3 Is Induced in Cancer-Associated Fibroblasts and Promotes
Angiogenesis in Oral Squamous Cell Carcinoma. PLoS One.. 2016.04; 11(4); e0154112
12. Suzuki M, Deno M, Myers M, Asakage T, Takahashi K, Saito K, Mori Y, Saito H, Ichikawa Y, YamamotoMitani N, Miyashita M. Anxiety and depression in patients after surgery for head and neck cancer in Japan.
Palliat Support Care.. 2016.05; 299(8); 1110-1120
13. Okuyama K, Michi Y, Mizutani M, Yamashiro M, Kaida A, Harada K. Clinical study on mandibular
fracture after marginal resection of the mandible. Oral Surg Oral Med Oral Pathol Oral Radiol. 2016.05;
121(5); 461-467
14. Badri MK, Zhang H, Ohyama Y, Venkitapathi S, Kamiya N, Takeda H, Ray M, Scott G, Tsuji T, Kunieda
T, Mishina Y, Mochida Y. Ellis van Creveld2 is required for postnatal craniofacial bone development. Anat
Rec (Hoboken).. 2016.05; 299(8); 1110-1120
15. Yoshitake H, Kayamori K, Wake S, Sato F, Kino K,Harada K. Synovial chondromatosis of the right side
temporomandibular joint extending to the middle cranial fossa: A case report with 7-year postoperative
follow up and expression of a biomarker of cell proliferative activity. Anat Rec (Hoboken).. 2016.06; 20;
133-137
16. Kondo Y, Ohno T, Nishii N, Harada K, Yagita H, Azuma M. Diﬀerential contribution of three immune
checkpoint (VISTA, CTLA-4, PD-1) pathways to antitumor responses against squamous cell carcinoma.
Oral Oncol.. 2016.06; 57; 54-60
17. Ohyama Y, Lin JH, Govitvattana N, Lin IP, Venkitapathi S, Alamoudi A, Husein D, An C, Hotta H,
Kaku M, Mochida Y. FAM20A binds to and regulates FAM20C localization. Sci Rep.. 2016.06; 6; 27784
18. Wake S, Yoshitake H, Kayamori K, Izumo T, Harada K. Expression of CD90 decreases with progressionof synovial chondromatosis in the temporomandibular joint. Cranio.. 2016.07; 34(4); 250-256
19. Jaha H, Husein D, Ohyama Y, Xu D, Suzuki S, Huang GT, Mochida Y. N-terminal Dentin Sialoprotein fragment induces type I collagen production and upregulates dentinogenesis marker expression in
osteoblasts. Biochemistry and Biophysics Reports.. 2016.07; 6; 190-196
20. Shuji Hibiya, Kiichiro Tsuchiya, Ryohei Hayashi, Keita Fukushima, Nobukatsu Horita, Sho Watanabe,Tomoaki Shirasaki, Ryu Nishimura, Natsuko Kimura, Tatsunori Nishimura, Noriko Gotoh, Shigeru Oshima, Ryuichi Okamoto, Tetsuya Nakamura, Mamoru Watanabe. Long-term inﬂammation transforms
intestinal epithelial cells of colonic organoids. J Crohns Colitis. 2016.10;
21. Masanori Kobayashi, Shigeru Oshima, Chiaki Maeyashiki, Yoichi Nibe, Kana Otsubo, Yu Matsuzawa,
Yasuhiro Nemoto, Takashi Nagaishi, Ryuichi Okamoto, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru
Watanabe. The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonicdevelopment. Sci Rep. 2016.11; 6; 36780
22. Morita T, Uzawa N, Mogushi K, Sumino J, Michikawa C, Takahashi KI, Myo K, Izumo T, Harada K.
Characterizing Genetic Transitions of Copy Number Alterations and Allelic Imbalances in Oral Tongue
Carcinoma Metastasis. Genes Chromosomes Cancer.. 2016.12; 55; 975-986
23. Terauchi M, Inada T, Tonegawa A, Tamura A, Yamaguchi S, Harada K, Yui N. Supramolecular inclusion
complexation of simvastatin with methylated β-cyclodextrins for promoting osteogenic diﬀerentiation.
Int. J. Biol. Macromol.. 2016.12; 93(PtB); 1492-1498― 82 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction[Conference Activities & Talks]

1. Morita K, Naruto T, Tanimoto K, Yasukawa C, Oikawa Y, Masuda K, Imoto I, Inazawa J, Omura K,
Harada H. Simultaneous detection of both single nucleotide variations and copy number alterations by
next-generation sequencing in gorlin syndrome. . The 13th International Congress of Human Genetics
2016.04.03 Kyoto
2. Uezono M, Takakuda K, Hiratsuka T, Ogasawara T, Oshima S, Sato T, Kikuchi M, Suzuki S, Harada K,
Moriyama K. Mechanical properties of newly formed bone around subperiosteal devices evaluated withmicro-indentation test.. 10th World Biomaterials Congress (WBC) 2016.05.17 Montreal, Canada.
3. Masahiko Terauchi, Takasuke Inada, Atsushi Tamura, Satoshi Yamaguchi, Nobuhiko Yui. Osteoregeneration by supramolecular sulfonated polurotaxane/BMP-2 polyelectrolyte complexes in mouse calvarial
defect model.. 11th International Conference Medical Applications of Novel Biomaterials and Nanotechnology, 5thInternational Conference Smart and Multifunctional Materials Structures & Systems. Centro
Congressi 2016.06.05 Italy
4. Uezono M, Takakuda K, Hiratsuka T, Ogasawara T, Kikuchi M, Harada K, Suzuki S, Moriyama K..
Yield Strength of Newly Formed Bone Around Subperiosteal Anchorage Devices. 94th General Session &Exhibition of International Association for Dental Research. 2016.06.22 Seoul,Korea
5. Kondo Y, Ohno T, Azuma M. Combined VISTA and CTLA-4 immune-checkpoint blockade enhances
antitumor immunity. 94th Annual Meeting of the International Association for Dental Research (IADR
2016) 2016.06.22 Seoul, Korea
6. Horita N, Demitrack ES, Giﬀord GB, Keeley TM, Siebel CW, Samuelson LC. Notch1 and Notch2 regulate
epithelial cell proliferation in mouse and human gastric corpus. The 2016 Gordon Research Conferenceon Notch Signaling in Development, Regeneration & Disease 2016.08.01 Lewiston（USA）
7. Yoshitake H. Development of the new instrument for measurement of mouth opening and mouth-opening
pathway. 23rd Congress of the European Association for Cranio Maxillo-Facial Surgery 2016.09.13 London,UK
8. Yoshitake H. Development of the new TMJ rehabilitation device that induces a protrusive sliding movement of the mandibular condyle. 23rd Congress of the European Association for Cranio Maxillo-Facial
Surgery 2016.09.13 London, UK
9. Masahiko Terauchi, Atsushi Tamura, Nobuhiko Yui.. Supramolecular Sulfonated Polyrotaxanes complexedwith Bone Morphogenetic Protein-2 for Bone Tissue Engineering. International Dental Materials Congress2016 2016.11.04 Indonesia
10. Ogasawara T, Uezono M, Takakuda K, Kikuchi M, Hiratsuka T, Suzuki S, Moriyama K. Finite element
analysis of bone bonding strength for the orthodontic subperiosteal devices. The 75th Annual Meeting of
the Japanese Orthodontic Sciety 2016.11.07 Tokushima[Patents]
1. MEDICAL DEVICE, DEVICE STRUCTURE FOR DENTAL, HEAD AND NECK SURGICAL AND
ORTHOPEDIC USE, AND METHOD FOR JOINING MEDICAL DEVICE TO BONE , Announcement
Number：WO 2016136913― 83 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

Maxillofacial Orthognathics
Professor

Keiji MORIYAMA

Associate Professor
Junior Associate Professor
Assistant Professor

Shoichi SUZUKI
Takuya OGAWA
Michiko TSUJI, Norihisa HIGASHIHORI,
Jun MIYAMOTO, Yukiho KOBAYASHI,
Yosuke ITO

Clinical Fellow

Rina HIKITA, Yuko KOMAZAKI,Kenji OGURA, Yuki TAKAHASHI,
Naoki KOUDA, Sakiko AKIYAMA,
Takuya ASAMI

Post-doctoral Fellow

Chiho KADOTA, Masayoshi UEZONO

Graduate Student

Keiko MURAMOTO, Akitsu IKEDA,
Kouhei YAHIRO, Ayumi SHOJI,
Taizo HIRATSUKA, Entei RIN,
Miyu ARAKI, Takeshi OGASAWARA,
Yoshihiro YAMAGATA, Aung Bhone Myat,
Hiroyuki KAMIMOTO, Kyoko HIRABAYASHI,
Kenta FUNAHASHI, Hideyuki YOSHIZAWA,
Wu Yu Yun, Kaori KADOWAKI,
Soonhwa KANG, Sayuri SAITO,
Hidekazu MATSUMOTO, Takayuki MIYAZAKI,Teramoto Iida Airy, Phyo Thiha,
Nay Myo Min Swe
Graduate International Research Student
Naomi YAMAMOTO,Rie KINOSHITA,
Ruriko NAKAMURA, Misato HANDA,
Syuhei AKIYAMA, Megumi ARIMURA,
Asuka TAMURA, Cheng Shih-wei Eric,
Kenjiro MATSUMURA, Yuri BABA,
Yukiko KINOSHITA, Chisato TOMINAGA,
Daichi HAYAKA, Thili HLA Myint,
Yuki NAKAZAWA, Sahori MATSUNO
Visiting Research Scholar
Tsasan Tumurkhuu, Lisa R.Amir
Part-time Lecturer
Tatsuo KAWAMOTO, Naoto SUDA,
Takafumi SUSAMI, Tamiko TERASHIMA,
Yoshiyuki KATO, Yasuo ISHIWATA,
Yoshiyuki BABA, Toshimoto TENGAN,
Masahiko YOKOZEKI, Shigetoshi HIYAMA,
Shigeki TAKAHASHI, Momotoshi SHIGA,
Hiroki FUKUOKA, Junichi TAKADA,
Maristela Sayuri INOUE, Keiichi KATAOKA,
Hiroyuki SUZUKI, Ryo MARUOKA― 84 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction(1)

Research

１）Basic and clinical studies of cleft lip and/or palate and other congenital craniofacial conditions
２）Morphological and physiological studies of facial deformity３）Physiological study about control mechanism of stomatognathic function４）Functional MRI study in the craniofacial region５）Clarify the factors of malocclusion with epidemiological technique(2)

Education

The goal of the program of Maxillofacial Orthognathics is to provide information related to craniofacial growthand development, and stomatognathic function in order to develop basic knowledge and skills for the treatmentof the patients with a wide variety of malocclusion. It also provides valuable information of diagnosis and treatment planning for orthodontic and orthognathic therapies of the patients with jaw deformities and congenital
craniofacial anomalies.
Comprehensive care by a team of specialists including maxillofacial surgeons, orthodontists, speech therapists
etc. is needed for the treatment of the patients with cleft lip and palate and other craniofacial anomalies. The
Graduate Program provides the clinical education of orthodontics as a part of the multi-disciplinary approachfor such patients.

(3)

Clinical Performances

In the Clinic, we treat a large number of patients presenting a variety of malocclusions to be assigned togroup practice in order to gain valuable experience in diagnosis, treatment planning, orthodontic therapy, andpatient management. Especially for patients born with cleft lip and/or palate and who need craniofacial andorthognathic surgery, we have clinical meetings and conferences for the comprehensive care through a teamapproach with maxillofacial surgeons, maxillofacial prosthodontists and speech therapists. We also provide
supportive counseling to families who have members with congenital anomalies before the treatment.

(4)

Publications[Original Articles]
1. Koda N, Sato T, Shinohara M, Ichinose S, Ito Y, Nakamichi R, Kayama T, Suzuki H, Moriyama K,
Asahara H . The transcription factor mohawk homeobox regulates homeostasis of the periodontal ligament.
Development. 2016;
2. Lin W, Ezura Y, Izu Y, Aryal SA, Kawasaki M, Chantida PN, Moriyama K, Noda M. Proﬁlin Expression Is
Regulated by Bone Morphogenetic Protein (BMP) in Osteoblastic Cells. Journal of Cellular Biochemistry.
2016.03; 117(3); 621-628
3. Morishita M, Muramatsu T, Suto Y, Hirai M, Konishi T, Hayashi S, Shigemizu D, Tsunoda T, Moriyama
K, Inazawa J. Chromothripsis-like chromosomal rearrangements induced by ionizing radiation using proton
microbeam irradiation system. Oncotarget. 2016.03; 7(9); 10182-10192
4. Duarte C, Kobayashi Y, Morita J, Kawamoto T, Moriyama K. A preliminary investigation of the eﬀectof relaxin on bone remodelling in suture expansion. The European Journal of Orthodontics. 2016.05;
5. Sato M, Baba Y, Haruyama N, Higashihori N, Tsuji M, Suzuki S, Moriyama K. Clinicostatistical analysis
of congenitally missing permanent teeth in Japanese patients with cleft lip and/or palate Orthodontic
Waves. 2016.06; 75(2); 41-45
6. Suzuki H, Ito Y, Shinohara M, Yamashita S, Ichinose S, Kishida A, Oyaizu T, Kayama T, Nakamichi
R, Koda N, Yagishita K, Lotz MK, Okawa A, Asahara H.. Gene targeting of the transcription factor
Mohawk in rats causes heterotopic ossiﬁcation of Achilles tendon via failed tenogenesis. Proceedings of
the National Academy of Sciences of the United States of America. 2016.07; 113(28); 7840-7845― 85 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

7. Sato C, Ogawa T, Tsuge R, Shiga M, Tsuji M, Baba Y, Kosaki K, Moriyama K. Systemic and maxillofacialcharacteristics of 11 Japanese children with Russell-Silver syndrome. Congenit Anom (Kyoto). 2016.09;
56(5); 217-225
8. Dei A, Miyamoto JJ, Takada J, Ono T, Moriyama K. Evaluation of blood ﬂow and electromyographic
activity in the perioral muscles. European Journal of Orthodontics. 2016.10; 38(5); 525-531
9. Tumurkhuu T, Fujiwara T, Komazaki Y, Kawaguchi Y, Tanaka T, Inazawa J, Ganburged G, Bazar A,
Ogawa T, Moriyama K. Association between maternal education and malocclusion in Mongolian adolescents: a cross-sectional study. BMJ Open. 2016.11; 6(11);
10. Ogura K, Iimura T, Makino Y, Sugie-Oya A, Takakura A, Takao-Kawabata R, Ishizuya T, Moriyama K,
Yamaguchi A. Short-term intermittent administration of parathyroid hormone facilitates osteogenesis by
diﬀerent mechanisms in cancellous and cortical bone. Bone Reports. 2016.12; 5; 7-14
[Books etc]
1. Kobayashi Y, Duarte C, Moriyama K.. Biomarkers in Bone Disease. Part of the series Biomarkers in
Disease: Methods, Discoveries and Applications. 2016.07 (ISBN : 978-94-007-7745-3)[Conference Activities & Talks]
1. Koda N, Shinohara M, Nakamichi R, Ito Y, Ichinose S, Moriyama K, Asahara H. Roles of Transcriptional
Factor Mohawk in Periodontal Ligament. IBMS Herbert Fleisch Workshop 2016.02.28 Brugge,Belgium
2. Hikita R, Matsuno S, Asami T, Ogawa T, Baba Y, Tsuji M, Moriyama K. Systemic and cranimaxillofacialcharacteristics of patients with Williams syndrome. International Congress of Human Genetics 2016.
2016.04.03 Kyoto
3. Tsuji M, Ogura K, Hikita R, Kobayashi Y, Moriyama K. Maxillofacial morphological characteristics of two
Japanese patients with chromosome 18p deletion syndrome. International Congress of Human Genetics
2016. 2016.04.03 Kyoto
4. Uezono M, Takakuda K, Hiratsuka T, Ogasawara T, Oshima S, Sato T, Kikuchi M, Suzuki S, Harada K,
Moriyama K. Mechanical properties of newly formed bone around subperiosteal devices evaluated withmicro-indentation test.. 10th World Biomaterials Congress (WBC) 2016.05.17 Montreal, Canada.
5. Ikeda A, Miyamoto JJ, Usui N, Taira M, Moriyama K.. Eﬀect of mastication on satiety using an attentional bias task. . 94th General Session & Exhibition of International Association for Dental Research.
2016.06.22 Seoul,Korea
6. Uezono M, Takakuda K, Hiratsuka T, Ogasawara T, Kikuchi M, Harada K, Suzuki S, Moriyama K..
Yield Strength of Newly Formed Bone Around Subperiosteal Anchorage Devices. 94th General Session &Exhibition of International Association for Dental Research. 2016.06.22 Seoul,Korea
7. Koda N, Shinohara M, Nakamichi R, Ito Y, Ichinose S, Asahara H, Moriyama K. Roles of Transcriptional
Factor Mohawk in Periodontal Ligament. 94th General Session & Exhibition of International Association
for Dental Research. 2016.06.22 Seoul,Korea
8. Lin W, Ezura Y, Izu Y, Moriyama K, Noda M.. Role of Proﬁlin1 in BMP-induced Activities in Skeletal
Cells. 94th General Session & Exhibition of International Association for Dental Research. 2016.06.22
Seoul,Korea
9. The Dental Education Consortium to promote healthy longevity -Second report- Agendas after the second
year. 2016.07.02
10. Moriyama K. Dental Equilibrium Revisited : Soft Tissue Considerations in Treatment of Dentofacial
Deformity. The 10th Asia Paciﬁc orthodontic Conference and 11th Annual Meeting of the Indonesian
Association of Orthodontists 2016.09.03 Bali,Indonesia
11. Ito Y, Takahashi Y, Hikita R, Watanabe C, Akiyama S, Higashihori N, Moriyama K. Long-term changes
in clinical crown height after surgical orthodontic treatment for mandibular prognathism. The 10th Asia
Paciﬁc orthodontic Conference and 11th Annual Meeting of the Indonesian Association of Orthodontists
2016.09.03 Bali,Indonesia― 86 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

12. Higashihori N, Hikita R, Moriyama K. Surgical-orthodontic treatment for facial asymmetry patient whose
occlusal plane canted opposite to the mandibular deviation. The 10th Asia Paciﬁc orthodontic Conference
and 11th Annual Meeting of the Indonesian Association of Orthodontists 2016.09.03 Bali,Indonesia
13. Akiyama S, Ogura K, Miyamoto JJ, Komazaki Y, Takada J, Moriyama K. Clinical study of Marfan
syndrome patients: intraoral characteristics.. The 49th Annual Scientiﬁc Congress,Korean Association of
Orthodontists 2016.10.07 Seoul,Korea
14. Baba Y, Komazaki Y, Miyamoto J, Ogura K, Ahiko N, Baba Y, Tuji M, Moriyama K. Maxillofacial
morphology of 4 cases of Marfan-related disorders in mixed dentition stage. The 49th Annual Scientiﬁc
Congress,Korean Association of Orthodontists 2016.10.07 Seoul,Korea
15. Arimura M, Komazaki Y, Miyamoto JJ, Ogura K, Kobayashi Y, Moriyama K. Clinical study of JapaneseMarfan syndrome patients: craniofacial morphology. The 49th Annual Scientiﬁc Congress,Korean Association of Orthodontists 2016.10.07 Seoul,Korea
16. Lin W. Role of Proﬁlin1 in Skeletal Cells. Tri-University Consortiume 5th Tri-University Consortium
2016.10.27 Peking,China
17. Ogawa T, Muramoto K, Moriyama K. Long-tern observation of maxillofacial growth in a Klippel-TrenaunayWeber syndrome patienr. The 75th Annual Meeting of the Japanese Orthodontic Sciety 2016.11.07
18. Shoji A, Ono T, Nakashima T, Moriyama K. Elucidation of the mechanism on alveolar bone remodeling during orthodontic tooth movement. The 75th Annual Meeting of the Japanese Orthodontic Sciety
2016.11.07
19. Asami T, Ito Y, Akiyama S, Hirabasyshi K, Ogasawara T, Kurohara K, Moriyama K. A case report ofsurgical orthodontic treatment fot monozygotic twins with mandibular prognathism. The 75th Annual
Meeting of the Japanese Orthodontic Sciety 2016.11.07 Tokushima
20. Ogura K, Iimura T, Yamagushi A, Moriyama K. Eﬀect of intermittent administration of parathyroid
hormone on osteogenesis. The 75th Annual Meeting of the Japanese Orthodontic Sciety 2016.11.07
Tokushima
21. Yahiro K, Higashiori N, Moriyama K. The roles of histone methyltransferase Setdb1 during craniofacial
development. The 75th Annual Meeting of the Japanese Orthodontic Sciety 2016.11.07 Tokushima
22. Ogasawara T, Uezono M, Takakuda K, Kikuchi M, Hiratsuka T, Suzuki S, Moriyama K. Finite element
analysis of bone bonding strength for the orthodontic subperiosteal devices. The 75th Annual Meeting of
the Japanese Orthodontic Sciety 2016.11.07 Tokushima
23. Kobayashi Y, Tsuji M, Hikita R, Ogura K, Moriyama K. Analysis of maxillofacial morphology of craniosynostosis patients: comparison between Apert syndrome and Crouzon syndrome. The 75th Annual
Meeting of the Japanese Orthodontic Sciety 2016.11.07 Tokushima
24. Tamura A, Ogura K, Miyamoto J, Komazaki Y, Takada J, Yoshizawa H, Go I, Tsuji M, Moriyama K.
Clinical study of Marfan syndrome patients: medical symptoms. The 75th Annual Meeting of the Japanese
Orthodontic Sciety 2016.11.07 Tokushima
25. Takahashi Y, Higashihori N, Moriyama K. treatment of a high-angle class II malocclusion with condylar
resorption using an orthodontic anchor plate. The 75th Annual Meeting of the Japanese Orthodontic
Sciety 2016.11.07 Tokushima
26. Kobayashi Y, Moriyama K. Identiﬁcation of micro RNA responsible for the onest of craniosynostosis andcranial sutural development. The 75th Annual Meeting of the Japanese Orthodontic Sciety 2016.11.07
Tokushima
27. Arimura M, Komazaki Y, Miyamoyo J, Ogura K, Takada J, Cheng E, Baba Y, Teramoto A, Funahashi
K, Kobayashi Y, Moriyama K. Clinical study of Marfan syndrome patients:Craniofacial morphology. The
75th Annual Meeting of the Japanese Orthodontic Sciety 2016.11.07 Tokushima
28. Akiyama S, Ogura K, Miyamoto J, Komazaki Y, Takada J, Go I, Tsuji M, Moriyama K. Clinical studyof Marfan syndrome patients: Intraoral characteristics. The 75th Annual Meeting of the Japanese Orthodontic Sciety 2016.11.07 Tokushima― 87 ―

Maxillofacial and Neck Reconstruction

Maxillofacial and Neck Reconstruction

29. Baba Y, Komazaki Y, Miyamoto J, Ogura K, Takada J, Hirabayashi K, Ahiko N, Baba Y, Kobayashi Y,
Tsuji M, Moriyama K. Maxillofacial morphology of 4 cases of Marfan-related disorders in mixed dentition
stage. The 75th Annual Meeting of the Japanese Orthodontic Sciety 2016.11.07 Tokushima
30. Hikita R, Tsuji M, Kobayashi Y, Ogura K, Moriyama K. Craniofacial characteristics of two Seathre-Chozen
syndrome cases. The 75th Annual Meeting of the Japanese Orthodontic Sciety 2016.11.07 Tokushima
31. Matsumura K, Ogura K, Miyamoto J, Komazaki Y, Takada J, Kaminoto H, Hikita R, Moriyama K.
Clinical study of Marfan syndrome patients: Morphological characteristics of pharyngeal airway andintraoral soft tissue. The 75th Annual Meeting of the Japanese Orthodontic Sciety 2016.11.07 Tokushima
32. Araki M, Komazaki Y, Ogawa T, Tumurkhuu T, Ganburged G, Bazar A, Fujiwara T, Moriyam K. Association between malocclusion and oral health-related quality of life in Mongolian adolescents. The 75th
Annual Meeting of the Japanese Orthodontic Sciety 2016.11.07 Tokushima
33. Asami T, Ito Y, Moriyama K. Surgical orthodontic treatment of high-angle Class II malocclusion. The
29th Taiwan Association of Orthodontists Annual Meeting 2016.12.02 Taipei, Taiwan[Patents]
1. MEDICAL DEVICE, DEVICE STRUCTURE FOR DENTAL, HEAD AND NECK SURGICAL AND
ORTHOPEDIC USE, AND METHOD FOR JOINING MEDICAL DEVICE TO BONE , Announcement
Number：WO 2016136913― 88 ―

Maxillofacial
Maxillofacial andand Neck
Neck Reconstruction
Reconstruction

Maxillofacial Prosthetics

2016

April

Professor
TANIGUCHI Hisashi
Junior Associate Professor
SUMITA Yuka
Assistant Professor
HATTORI Mariko
OTOMARU Takafumi
Clinical Staﬀ
HARAGUCHI Mihoko
MURASE Mai
YOSHI Shigen
Graduate Student
KANAZAKI Ayako
ELBASHTI Mahmoud Ellarousi
YANAGI Ayaka
SAID Mohamed Moustafa
ASWEHLEE Amel Mohamed
LI Na
KAMIYANAGI Ayuko
KELIMU Shajidan
YEERKEN Yesiboli
Part-time Lecturer
SATO Iwao
NOMURA koji
Part-time Special Student
HATANO Noriko
WATANABE Mao
KAMARUDIN Kamarul Hisham
ZHANG Manjin
LIU Rongguang
Speech-Language-Hearing Therapist
MIBU Michiko― 89 ―

Maxillofacial andReconstruction
Maxillofacial
and Neck
Neck Reconstruction(1)

OutlineDepartment of Maxillofacial Prosthetic is the special unit of the prosthodontic and/or prosthetic treatment
for patients with defects in oral and/or maxillofacial regions. The main objective of this course is to provide
students with opportunity to gain sound understanding of the restoration of functional and esthetic disordersof oral and/or maxillofacial areas that are caused by congenital developmental or acquired diseases by meansof the high-advanced dental and medical cares.
(2)

Research

Our department is the special unit for the prosthodontic treatment for patients with congenital or acquired
defects in head and neck regions. The main goal of the research is to establish a novel theory and feedback it
to the clinic to improve the quality of life of each patient. In this respect, we are focusing on several projects.
Diagnosis of functional impairment in patients with a maxillofacial defectTreatments for functional rehabilitation of patients with a maxillofacial defect
Masticatory evaluation in patients with a maxillofacial defectSpeech evaluation in patients with a maxillofacial defectDevelopment of new materials for facial prosthesis(3)

Publications[Original Articles]
1. Aimaijiang Y, Otomaru T, Taniguchi H. Relationships between perceived chewing ability, objective masticatory function and oral health-related quality of life in mandibulectomy or glossectomy patients with adento-maxillary prosthesis. Journal of Prosthodontic Research. 2016.04; 60(2); 92-97
2. Haraguchi M, Michi Y, Tanaka K, Harada H, Sumita YI, Harada K, Okazaki M, Taniguchi H. Appliances
aimed improvement of oral form in postoperative oral cancer patients - Two modiﬁed oral stent cases -.
2016.05; 42(1); 13-20
3. Otomaru T, Sumita YI, Aimaijiang Y, Munakata Y, Tachikawa N, Kasugai S, Taniguchi H. Rehabilitationof a bilateral maxillectomy patient with a free ﬁbula osteocutaneous ﬂap and with an implant-retained
obturator: a clinical report. Journal of Prosthodontics. 2016.06; 25(4); 341-348
4. Nomura K, Otomaru T, Kosaka M, Yoshi S, Said M, Taniguchi H. Prosthodontic rehabilitation of anadolescent patient with maxillary resection up to 7 years -a case report- Maxillofacial Prosthetics. 2016.06;
39(1); 15-20
5. Elbashti ME, Hattori M, Sumita YI, Aswehlee A, Yoshi S, Taniguchi H. Creating a digitized database of
maxillofacial prostheses (obturators): A pilot study. Journal of Advanced Prosthodontics. 2016.06; 8(3);219-223
6. Li N, Otomaru T, Said M, Munakata M, Tachikawa N, Kasugai S, Taniguchi H. Prosthodontic rehabilitation in a maxillectomy patient with an implant-supported overdenture: a clinical report. Cresco Journalof Clinical Case Reports. 2016.08; 1(1); 1-7
7. Hattori M, Sumita YI, Elbashti ME, Kurtz KS, Taniguchi H. Eﬀect of Experimental Palatal Prosthesis
on Voice Onset Time. J Prosthodont. 2016.08;
8. Oki M, Kanazaki A, Taniguchi H. Osteoradionecrosis following carbon ion radiotherapy: Case history
report of a soft palate defect. International Journal of Prosthodontics. 2016.09; 29(5); 448-450
9. Elbashti ME, Sumita YI, Aswehlee AM, Haraguchi M, Otomaru T, Hattori M, Taniguchi H. Preschool
and School Phases of Postmaxillectomy Prosthetic Rehabilitation in a Child: A Clinical Report. J
Prosthodont. 2016.10;
10. Said M, Otomaru T, Aimaijiang Y, Li N, Taniguchi H. Association between masticatory function and oral
health-related quality of life in partial maxillectomy patients. International Journal of Prosthodontics.
2016.11; 29(6); 561-564― 90 ―

Maxillofacial
Maxillofacial andand Neck
Neck Reconstruction
Reconstruction

11. Elbashti ME, Aswehlee A, Hattori M, Sumita YI, Taniguchi H. The role of digitization in the rapid
reproduction of an obturator in a frail elderly patient. International Journal of Prosthodontics. 2016.11;29(6); 592-594
12. Sumita YI, Hattori M, Shigen Y, Taniguchi H. Facial prosthesis using an acrylic base for a patient witha large facial defect. JSM Dental Surgery. 2016.11; 1(1); 1006
13. Kanazaki A, Otomaru, Said M, Li N, Taniguchi H. Eﬀect of chewing on body sway in patients wearing
dento-maxillary prosthesis after head and neck tumor resection. Maxillofacial Prosthetics. 2016.12; 39(2);53-59
14. Sumita YI. Consideration point for the morphological change in a long-term follow up Maxillofacial Prosthetics. 2016.12; 39(2); 68-71
15. Yoshi S, Sumita YI, Hattori M, Ohbayashi N, Kurabayashi T, Taniguchi H. The morphological evaluationof denture space in glossectomy patients Maxillofacial Prosthetics. 2016.12; 39(2); 60-67[Conference Activities & Talks]
1. Sakata T, Matsumoto Y, Haraguchi M, Watanabe H, Nanba Y, Adachi T, Kaida A, Miura M, Yoshimura
R, Taniguchi H. A clinical report of perioperative oral care by a dental hygienist in brachytherapy fora tongue cancer patient. The 34th Annual Meeting of Japanese Society of Oral Oncology 2016.01.22
Kanagawa
2. Kamiyanagi A, Sumita YI, Taniguchi H, Chikai M, Kimura K, Seki Y, Ino S. Evaluation of the acoustic
characteristics of swallowing and expiratory sounds using throat microphone with AE sensor . Tsukuba
BME Collaboration Forum 2016 2016.01.22
3. Nagai H, Haraguchi M, Sumita YI, Taniguchi H. Satisfaction improvement with a stent in postoperative
edentulous mandibulectomy patient. 88th Annual Scientiﬁc Meeting of American Prosthodontic Scoeity
2016.02 Chicago, USA
4. Said M, Otomaru T, Yeerken Y, Taniguchi H. Prosthetic treatment outcomes in partial maxillectomy patients with and without oronasal communication. 88th Annual Scientiﬁc Meeting of American Prosthodontic Scoeity 2016.02 Chicago, USA
5. Elbashti ME, Aswehlee AM, Hattori M, Sumita YI, Taniguchi H. The role of digital technologies in maxillofacial prosthetic 3D presentations. 11th Biennial Meeting of the International Society for Maxillofacial
Rehabilitation 2016.05.04 Belgrade, Serbia
6. Elbashti ME, Hattori M, Patzelt SBM, Schulze D, Sumita YI, Taniguchi H. Evaluating the feasibility andaccuracy of digitizing edentulous maxillectomy defects: A comparative study. 11th Biennial Meeting of
the International Society for Maxillofacial Rehabilitation 2016.05.04 Belgrade, Serbia
7. Elbashti ME, Said M, Aswehlee AM, Hattori M, Sumita YI, Taniguchi H. Maxillofacial prosthetic literaturein prosthodontic journals: A 10-year observation. 11th Biennial Meeting of the International Society for
Maxillofacial Rehabilitation 2016.05.04 Belgrade, Serbia
8. Elbashti ME. Application of digital technology in prosthetic rehabilitation of head and neck cancer patients. 52nd Tokyo International Exchange Center-TIEC Research and Presentation 2016.05.14 Tokyo,
Japan
9. Otomaru T, Oki M, Yoshi S, Suzuki T, Taniguchi H. Considerration of a trial method to fabricate anindividual lead shield in radiotherapy of eyelid tumor using three dimensional digital technology. 33rd
Annual Meeting of Japanese Academy of Maxillofacial Prosthetics 2016.06 Niigata
10. Kamiyanagi A, Sumita YI, Nagai H, Murase M, Haraguchi M, Hattori M, Otomaru T, Taniguchi H. A
study of changes in the number of maxillectomy patients fabricated immediate surgical obturator at our
department. 33rd Annual Meeting of Japanese Academy of Maxillofacial Prosthetics 2016.06 Niigata
11. Li N, Otomaru T, Said M, Kamiyanagi A, Yeerken Y, Taniguchi H. Factors aﬀecting sleep quality inmaxillectomy patients with dento-maxillary prosthesis. 33rd Annual Meeting of Japanese Academy ofMaxillofacial Prosthetics 2016.06 Niigata― 91 ―

Maxillofacial andReconstruction
Maxillofacial
and Neck
Neck Reconstruction

12. Elbashti ME, Hattori M, Aswehlee AM, Sumita YI, Taniguchi H. Adoption and use of digital technologies in maxillofacial prosthetics in Japan. 33rd Annual Meeting of Japanese Academy of Maxillofacial
Prosthetics 2016.06 Niigata
13. Sumita YI. Consideration point for the morphological change in a long-term follow-up. 21st JAMP
2016.06.04 Niigata
14. Haraguchi M, Sumita YI, Taniguchi H. Immediate surgical obturator treatment for maxillectomy patients.
The 40th Annual Meeting of Japan Society for Head and Neck Cance 2016.06.09 Saitama
15. Hattori M, Sumita YI, Elbashti ME, Kelimu S, Aswehlee A, Taniguchi H. New powder-free palatogram
method using sandblast: In vitro examination. 2016 meeting of the International Association of Dental
Research - General Session 2016.06.25 Seoul, Korea
16. Churei H, Shrestha A, Sumita YI, Suzuki T, Matsubara H, Taniguchi H, Ueno T. Evaluation of an
obturator type of sports mouthguard for a maxillectomy sports-active patient. The 125th Annual Meetingof the Japan Prosthodontic Society 2016.07 Ishikawa
17. Murase M, Yanagi A, Sumita YI, Taniguchi H. Nutrition assessment for a maxillectomy patient frompreoperative period to insertion of deﬁnitive obturator period. The 125th Annual Meeting of the Japan
Prosthodontic Society 2016.07 Ishikawa
18. Yanagi A, Murase M, Sumita YI, Taniguchi H. Investigation of Nutritional Status using Mini Nutritional
Assessment-Short Form and Analysis of the Relevant Factors in Head and Neck Tumor/Cancer Patients.
The 125th Annual Meeting of the Japan Prosthodontic Society 2016.07 Ishikawa
19. Sumita YI. Consideration of the soft tissue can brush up the dental examination. The 125th Annual
Meeting of the Japan Prosthodontic Society 2016.07.10 Ishikawa
20. Hattori M. Prosthodontic treatment after maxillectomy and the spontaneously closed perforation. The
125th Annual Meeting of the Japan Prosthodontic Society 2016.07.10
21. Hattori M, Elbashti ME, Sumita YI, Taniguchi H. Visualization of voice sound in a patient after headand-neck cancer surgery. 12th International Voice Symposium Salzburg 2016.08.28 Salzburg, Austria
22. Elbashti ME, Aswehlee A, Hattori M, Sumita YI, Taniguchi H. Geometrical evaluation of prosthetic rehabilitation eﬀect on facial appearance of mandibulectomy patients. 2nd Annual Meeting of the International
Academy for Digital Dental Medicine 2016.09.04 Busan, Korea
23. Elbashti ME, Hattori M, Patzelt SBM, Schulze D, Aswehlee A, Sumita YI, Taniguchi H. Precision andtrueness of computerized optical impressions in maxillectomy defects: An in vitro 3D comparison. 2nd
Annual Meeting of the International Academy for Digital Dental Medicine 2016.09.04 Busan, Korea
24. Aswehlee A, ElbashtiME, Hattori M, Sumita YI, Taniguchi H. Feasibility and accuracy of non-contact 3D
digitizers in facial defect: An in vitro 3D comparison. 2nd Annual Meeting of the International Academy
for Digital Dental Medicine 2016.09.04 Busan, Korea
25. Hattori M, Aggarwal H, Kelimu S, Namba T, Nagai H, Sumita YI, Taniguchi H. Eﬀect of palatal prosthesison consonant duration of aﬀricate sounds. 40th European Prosthodontic Association and 65th German
Society for Prosthetic Dentistry and Biomaterials 2016.09.17 Halle, Germany
26. Sumita YI, Haraguchi M, Murase M, Yoshi S, Hattori M, Otomaru T, Taniguchi H. Prostheses for brachy
therapy in TMDU. The 23rd General Meeting of the Japanese for Dental Science 2016.10 Fukuoka
27. Sumita YI, Haraguchi M, Murase M, Yoshi S, Hattori M, Otomaru T, Taniguchi H. Immediate surgicalobuturator in TMDU. The 23rd General Meeting of the Japanese for Dental Science 2016.10 Fukuoka
28. Haraguchi M, Tanaka K, Sumita YI, Okazaki M, Taniguchi H. Prosthetic rehabilitation for a patient withextensive vascular malformation in the head and neck region. The 63rd Annual Meeting of the American
Academy of Maxillofacial Prosthetics 2016.10 San Diego, USA
29. Hatano N, Sumita YI, Otomaru T, Takano T, Taniguchi H. An application of maxillofacial prosthesis ina maxillectomy patient with edentulous maxillae and the ﬂap. The 63rd Annual Meeting of the American
Academy of Maxillofacial Prosthetics 2016.10 San Diego, USA― 92 ―

Maxillofacial
Maxillofacial andand Neck
Neck Reconstruction
Reconstruction

30. Li N, Otomaru T, Taniguchi H. Sleep survery in long-term head and neck cancer surviviors with dentomaxillary prostheses. The 63rd Annual Meeting of the American Academy of Maxillofacial Prosthetics
2016.10 San Diego, USA
31. Murase M, Sumita YI, Taniguchi H. A case of mandibular retrognathia with neuroﬁbromatosis type 1
and medication-related osteonecrosis of the jaw. The 63rd Annual Meeting of the American Academy ofMaxillofacial Prosthetics 2016.10 San Diego, USA
32. Nagai H, Haraguchi M, Hattori M, Sumita YI, Taniguchi H. Speech assessment in mandibulectomy patientswith stents. The 63rd Annual Meeting of the American Academy of Maxillofacial Prosthetics 2016.10 San
Diego, USA
33. Hattori M, Patzelt SBM, Sumita YI, Taniguchi H. Lateral embouchure impression in the dental treatmentof a saxophonist. 15.Symposium der Deutschen Gesellschaft für Musikphysiologie und Musikermedizin
2016.10.07 Berlin, Germany
34. Yagihara K, Ishii J, Katsurano M, Tsuchida E, Okamura T, Haraguchi M, Ishikawa A, Yagishita H, Izumo
T, Okabe S. Prognosis of tongue cancer patients treated with local resection plus skin graft -part 2-. 54th
Annual Meeting of Japan Society of Clinical Oncology 2016.10.22 Kanagawa
35. Suzuki A, Otomaru T, Taniguchi H, Nakata H, KAsugai S, Kuroda S. A modal analysis of ﬁxed implant
supported prosthetic devices of the six abutments embedded in edentulous maxillae. 20th Annual Meetingof Japanese Academy of Maxillofacial Implants 2016.12.03 Tokyo
36. Sumita YI. Functional improvements with maxillofacial prostheses. The 2nd Scientiﬁc Meeting of Japan
Proshodontic Society "Prostho ’16" 2016.12.11 Tokyo[Awards & Honors]
1. International Collage of Prosthodontists research grant award (Mahmoud Elbashti), International Collage
of Prosthodontists, 2016.04
2. 3rd place prize winner of poster presentation award of the 11th Biennial Meeting of the International
Society for Maxillofacial Rehabilitation-ISMR (Mahmoud Elbashti), International Society for Maxillofacial
Rehabilitation-ISMR, 2016.05
3. 2nd prize of best poster award of the 2nd Annual Meeting of the International Academy for Digital Dental
Medicine (Mahmoud Elbashti), International Academy for Digital Dental Medicine, 2016.09
4. First Place in the poster competition of the 63rd Annual Meeting of the American Academy of Maxillofacial
Prosthetics (Li Na) , 2016.10― 93 ―

Bio-Matrix

Bio-Matrix

Cell Biology

Professor : Takao Nakata
Associate Professor : Akihiro Inoue
Junior Associate Professor : Tomohiro Ishii
Assistant Professor : Toshifumi Asano
Technical Staﬀ : Satoko Nakamura(1)

OutlineWe started a new laboratory from April 2009. We are interested in the cellular responses to spatio-temporal
activation of signaling molecules. For this purpose, we took synthetic approaches combined with optogenetics.
We introduce the photo switches into cells, and analyze signaling systems quantitatively. Research will be
conducted by using molecular biology, molecular genetics, cell biology, theoretical biology, and live-imaging
techniques.
(2)

Research

We are studying cell signaling using optogenetics. We made photo-switch of various signaling proteins andintroduced them into cells. Parts of the cells were stimulated by blue lasers. The photo-switches were activatedlocally with in the cells and we observe the cell phenotypes by time-laps microscope using these techniques
we can understand molecular mechanisms of cell signaling in spatio temporal fashion and also can manipulate
cellular conditions using these switches.
(3)

Education

We teach histology and cell biology to 2nd year medical students. The courses are composed of sets of lecture
and laboratory study of tissues and organs. Our goal in undergraduate course is to provide students with
fundamental knowledge and skill to analyze microscopic samples of normal human body.
In new curriculum in lecture provide students information on ﬁne structure and hints or laboratory work thishelps the students to sketch the tissue in their laboratory work. we aimed our lecture provide fundamental
knowledge of human tissues and organ to learn clinical lectures. In laboratory work we adopt classical sketchof tissues because we believe it shows ability of students to search the place representive of area and extractessential structure. Evaluation depends on paper test, sketch and laboratory test.
In cell biology course we start a little bit advanced lectures such as cell death, cell cycle because we avoid
to teach the same contents they have learned in past year biology course. We also provide more stimulative
lectures in later half of the course such as autophagy zebraﬁsh genetics and mathematical model.

(4)

Publications[Misc]
1. Tomohiro Ishii, Takao Nakata. Light manipulation of intracellular Ca2+ signals by a genetically encoded
protein Experimental Medicine. 2016.03; 34(4); 601-606― 94 ―

Bio-Matrix

Bio-Matrix[Conference Activities & Talks]

1. Shiori Seki, Tomohiro Ishii, Takao Nakata. Optical control of intracellular Ca2+ signals and simultaneous
imaging of Ca2+ ﬂactuation and cellular events. The 121st Annual Meeting of the Japanese Associationof Anatomists 2016.03.28
2. Tomohiro Ishii, Takao Nakata. BACCS, a novel optogenetic tool controlling intracellular Ca2+ signals.
The 121st Annual Meeting of the Japanese Association of Anatomists 2016.03.28
3. Tomohiro Ishii, Takao Nakata. Optical control of Ca2+ signaling by a synthetic protein BACCS. International and Interdisciplinary Symposium 2016 "Towards a New Era of Cardiovascular Research" 2016.07.13
Tokyo Medical and Dental University, Tokyo
4. Tomohiro Ishii, Koji Sato, Toshiyuki Kakumoto, Shigenori Miura, Kazushige Touhara, Shoji Takeuchi,
Takao Nakata. Light control of intracellular Ca2+ signals by a genetically encoded protein, BACCS. The
39th Annual Meeting of the Japan Neuroscience Society 2016.07.21 Paciﬁco Yokohama, Yokohama
5. Tomohiro Ishii, Koji Sato, Takao Nakata. A novel optogenetic tool controlling intracellular Ca2+ signals.
8th Symposium on Optogenetics 2016.09.29 Keio University, Mita, Tokyo― 95 ―

Bio-Matrix

Bio-Matrix

Medical Biochemistry

Professor Yutaka Hata
Assistant Professor Hiroaki Iwasa
Assistant Professor Kyoko Arimoto-Matsuzaki
Assistant Professor Junichi Maruyama
Other two staﬀs(1)

Research

1) The biological and chemical approach to study the Hippo pathway that controls cell proliferation, cell
diﬀerentiation, and cell death.
2) Versatile roles of the tumor suppressive RASSF proteins3) Discovery and development of chemical compounds that suppress cancer stemness and metastasis
4) Discovery and development of chemical compounds that facilitate myogenesis and prevent muscle atrophy5) Physiological meanings of the formation of the stress granules in mammalian cells.

(2)

Education

１：Undergraduate course
We organaized the course of Biochemmistry for the undergraduate students.
２：Master course
We organized the course of Biochemmistry for the master students.
３：Others
We gave a lecture about metabolism of cancer cells.
We gave a lecture entitled "How is the life of human maintained?" for the students of Tokyo University of Foreign
Studies.

(3)

Lectures & Courses

1) Undergraduate
We organize the course, Medical Biochemistry . The students are requested through these courses to obtaina comprehensive integrated knowledge of human biochemistry, which is important to understand how healthis maintained and which molecular and biochemical events cause human diseases and underlie the rational
treatments.
2) Graduate and others
We are studying the signaling pathway that regulates cell proliferation, cell diﬀerentiation, cell polarity, and cell
death. This pathway is well conserved from ﬂy to human. The mutations of the components lead to oncogenesis
and organ malformation. Several recent studies suggest that this pathway is implicated in inﬂammation andcell diﬀerentiation such as adipogenesis, osteogenesis, and keratinocyte diﬀerentiation. The pathway plays animportant role in various human diseases and could be a new therapeutic target. We give lectures about― 96 ―

Bio-Matrix

Bio-Matrix

our current studies to graduate students and others, and provide graduate students with the opportunity toparticipate in them.
(4)

Publications[Original Articles]
1. Maruyama J, Kobayashi Y, Umeda T, Vandewalle A, Takeda K, Ichijo H, Naguro I. Osmotic stress induces
the phosphorylation of WNK4 Ser575 via the p38MAPK-MK pathway. Scientiﬁc Report. 2016.01; 6;18710
2. Arimoto-Matsuzaki K, Saito H, Takekawa M.. TIA1 oxidation inhibits stress granule assembly and sensitizes cells to stress-induced apoptosis. Nature Communications. 2016.01;
3. Nagashima S, Maruyama J, Kawano S, Iwasa H, Nakagawa K, Ishigami-Yuasa M, Kagechika H, Nishina
H, Hata Y. . Validation of chemical compound library screening for transcriptional co-activator with PDZbinding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer
Science. 2016.07; 107(6); 791-802
4. Katano T, Fukuda M, Furue H, Yamazaki M, Abe M, Watanabe M, Nishida K, Yao I, Yamada A, Hata
Y, Okumura N, Nakazawa T, Yamamoto T, Sakimura K, Takao T, Ito S. Involvement of Brain-Enriched
Guanylate Kinase-Associated Protein (BEGAIN) in Chronic Pain after Peripheral Nerve Injury. eNeuro.
2016.10; 3(5); 110-116
5. Nagashima S, Bao Y, Hata Y.. The Hippo pathway as drug targets in cancer therapy and regenerative
medicine. Current Drug Targets. 2016.11;[Books etc]
1. Iwasa H, Shimizu T, Hata Y.. Encyclopedia of Signaling Molecules RASSF6. Springer, (ISBN : 978-14419-0460-7)
2. Xu X, Kodaka M, Iwasa H, Hata Y. Encyclopedia of Signaling Molecules MAGI2/S-SCAM. SPringer,
3. Kodaka M, Xu X, Yang X,Maruyama J, Hata Y. SPringer Protocol Appication of split-GFP reassembly
assay to the study of the in vitro myogenesis and myofusion.. Springer,[Misc]
1. Yutaka Hata, Takeru Sawada. Hippo tumor suppressor pathway Journal of Molecular Targeted Therapy
for Cancer. 14(4); 10-16― 97 ―

Bio-Matrix

Bio-Matrix

Joint Surgery and Sports Medicine

Takeshi Muneta
Hideyuki Koga
Kazumasa Miyatake, Mio Udo
Department of Joint Reconstruction
Tomoyuki Mochizuki
Department of Cartilage Regeneration
Kunikazu Tsuji
Toshifumi Watanabe
Hiroki Katagiri
Yoshie Seki, Enichi Nakatsuru, Arata Yuki
Akimasa Kimura, Mikio Shioda, Kanehiro Hiyama
Mari Uomizu, Kim Minde, Etsuko Matsumura
Kei Inomata, Hiroko Ueki, Mai Katakura, Takashi Kondo, Shinji Hagio, Takashi Hoshino
Masaki Amemiya, Naoko Araya, Hiroaki Onuma
Miyoko Ojima, Kahaer Abula
Aiko Ymada, Risa Tada, Haruno Kuroda(1)

Research

1. Development and establishment of isolation and expansion of mesenchymal stem cells
2. Research of biological characteristics of mesenchymal stem cells
3. Development and improvement of treatment method of articular cartilage defect with mesenchymal stemcells
4. Development of treatment strategy to joint structure injuries with mesenchymal stem cells
5. Development of artiﬁcial cartilage and bone
6. Development of novel agents related to bone and cartilage metabolism
7. Genetical approach to bone and cartilage metabolism
8. Clarifying mechanism and control of post-injury and postoperative inﬂammation with tissue ﬁbrosis
9. Clarifying mechanism of joint pain with novel treatment strategy
10. Novel treatment strategy for cuﬀtear(2)

Lectures & Courses

We are working with the Orthopaedic and Spinal Surgery as a Department of Orthopaedic Surgery of University
Hospital. The doctors start to have education of orthopedic surgery as a member of the department from the
staﬀs of the department of Orthopaedic Surgery according to the orthopaedic education and training program
after completing the two-year fundamental education and training program as a junior resident. They experiencea lot of traumatic patients and deepen their basic orthopaedic skills for two-year junior orthopaedic training in
one of the branch hospitals every year. They expand their skills and obtain orthopaedic specialty educations in
the advanced two-year education and training program. After completing a six-year educational program of the― 98 ―

Bio-Matrix

Bio-Matrix

orthopaedic surgery, they are recommended to apply to the orthopedic specialist form the Japanese Orthopaedic
Association. They usually apply to enter the graduate school program after 4 or 5 years of clinical experience.
We encourage not only orthopedic doctors but doctors of other specialty, veterinarian doctors and physicaltherapists, etc to work with us.
(3)

Clinical Services & Other Works

Treatment for sports injuries
Prevention, conservative treatment and rehabilitation for sports injuries
Anatomic double-bundle anterior cruciate ligament (ACL) reconstruction for ACL injuries
Surgical treatment for knee multiple ligament injuries
Surgical treatment for meniscal injuries to restore meniscal function
Regenerative medicine for unrepairable meniscus and cartilage injuries
Treatment for osteoarthritis (OA)
Conservative approaches to early OA
Joint-sparing surgeries such as osteotomies for moderate OA
Total arthroplasties for severe OA
Clinical researches and clinical results for above-mentioned approaches have been presented at both domestic
and international congresses, as well as reported in Japanese and English articles.
(4)

Clinical PerformancesSports injuries
We have been performing double-bundle ACL reconstruction since 1994 as a pioneer, and reported good clinicaloutcomes regarding knee stability, ratio of return to sports and patients’ satisfaction. As for meniscal surgeries,
we have been trying to repair as much as possible to restore meniscal function. In addition, we have developed anovel surgical procedure to restore meniscal function for patients with post-meniscectomy and discoid meniscus.
We have also analyzed mechanisms and preventive methods for ACL injuries, and ﬁrst in the world clariﬁed
a detailed ACL injury mechanism. Based on the ﬁndings, various approaches to ACL injury prevention andrehabilitation after ACL reconstruction is being conducted.
Arthroplasties
We have developed a new total knee system called Actiyas, named after the combination of active and healed("iyas" in Japanese), which is speciﬁcally designed for Japanese. In order to develop this, we analyzed Japanesebone morphology, and this system is designed for more functional and "active" motion without knee pain,and eventually patients to be "healed". Ceramic is used for the femoral implant, by which we can expect
lesser implant wear, resulting in better long-term results. We perform simultaneous bilateral arthroplasties for
Bilateral OA patients, helping them earlier return to daily life.
Regenerative medicine for cartilage and meniscus injuries using synovial stem cells
Based on numerous basic researches performed in our laboratory, we started stem cell therapy for cartilage
injuries using synovial stem cells since April 2008. In this therapy, we take synovium from patients at day surgery,culture synovial stem cells at the cell processing center in our university, and transplant them arthroscopically.
The safety and eﬀectiveness had been already conﬁrmed. In addition, we also started a clinical trial of synovial
stem cell transplantation for unrepairable meniscal tear since August 2014.

(5)

Publications[Original Articles]
1. Takashi Ikeda, Junya Aizawa, Hiroshi Nagasawa, Ikuko Gomi, Hiroyuki Kugota, Keigo Nanjo, Tetsuya
Jinno, Tadashi Masuda, Sadao Morita. Eﬀects and feasibility of exercise therapy combined with branched― 99 ―

Bio-Matrix

Bio-Matrix

chain amino acid supplementation on muscle strengthening in frail and pre-frail elderly people requiringlong-term care: A crossover trial Applied Physiology, Nutrition, and Metabolism. 2016; 41(4); 438-445
2. Ryohei Takada, Tetsuya Jinno, Daisuke Koga, Yuki Yamauchi, Yoshinori Asou, Takeshi Muneta, Atsushi
Okawa. Limited signiﬁcance of screening computed tomography after cementless total hip arthroplasty
with highly cross-linked polyethylene at 7 to 10 years of follow-up Mod Rheumatol. 2016; 26(5); 757-760
3. Tsuyoshi Yamada, Toshitaka Yoshii, Satoru Egawa, Ryohei Takada, Takashi Hirai, Hiroyuki Inose, Tsuyoshi
Kato, Tetsuya Jinno, Atsushi Okawa. Drain tip culture is not prognostic for surgical site infection in spinal
surgery under prophylactic use of antibiotics Spine (Phila Pa 1976). 2016; 41(14); 1179-1184
4. Takanori Kokubun, Naohiko Kanemura, Kenji Murata, Hideki Moriyama, Sadao Morita, Tetsuya Jinno,
Hidetoshi Ihara, Kiyomi Takayanagi. Eﬀect of changing the joint kinematics of knees with a ruptured
anterior cruciate ligament on the molecular biological responses in a spontaneous healing in a rat model
Am J Sports Med. 2016; 44(11); 2900-2910
5. Ryohei Takada, Tetsuya Jinno, Daisuke Koga, Masanobu Hirao, Takeshi Muneta, Atsushi Okawa. Comparison of wear rate and osteolysis between annealed and remelted highly cross-linked polyethylene intotal hip arthroplasty. A 7 to 10 year follow-up Orthop Traumatol Surg Res. 2016; 102(6); 707-721
6. Yusuke Nakagawa, Ichiro Sekiya, Shimpei Kondo, Takashi Tabuchi, Shizuko Ichinose, Hideyuki Koga,
Kunikazu Tsuji, Takeshi Muneta. Relationship between MRI T1 rho value and histological ﬁndings ofintact and radially incised menisci in microminipigs. J Magn Reson Imaging. 2016.02; 43(2); 434-445
7. Yusuke Nakagawa, Takeshi Muneta, Koji Otabe, Nobutake Ozeki, Mitsuru Mizuno, Mio Udo, Ryusuke
Saito, Katsuaki Yanagisawa, Shizuko Ichinose, Hideyuki Koga, Kunikazu Tsuji, Ichiro Sekiya. Cartilage
Derived from Bone Marrow Mesenchymal Stem Cells Expresses Lubricin In Vitro and In Vivo. PLoS
ONE. 2016.02; 11(2); e0148777
8. Toshifumi Watanabe, Takeshi Muneta, Kazuyoshi Yagishita, Kenji Hara, Hideyuki Koga, Ichiro Sekiya.
Closed Suction Drainage Is Not Necessary for Total Knee Arthroplasty: A Prospective Study on Simultaneous Bilateral Surgeries of a Mean Follow-Up of 5.5 Years. J Arthroplasty. 2016.03; 31(3); 641-645
9. Katsuaki Yanagisawa, Takeshi Muneta, Nobutake Ozeki, Yusuke Nakagawa, Mio Udo, Ryusuke Saito,
Hideyuki Koga, Kunikazu Tsuji, Ichiro Sekiya. Weekly injections of Hylan G-F 20 delay cartilage degeneration in partial meniscectomized rat knees. BMC Musculoskelet Disord. 2016.04; 17(1); 188
10. Kazumasa Miyatake, Takeshi Muneta, Miyoko Ojima, Jun Yamada, Yu Matsukura, Kahaer Abula, Ichiro
Sekiya, Kunikazu Tsuji. Coordinate and synergistic eﬀects of extensive treadmill exercise and ovariectomy
on articular cartilage degeneration. BMC Musculoskelet Disord. 2016.05; 17(1); 238
11. Nobutake Ozeki, Takeshi Muneta, Hideyuki Koga, Yusuke Nakagawa, Mitsuru Mizuno, Kunikazu Tsuji,
Y Mabuchi, C Akazawa, E Kobayashi, K Matsumoto, K Futamura, T Saito, Ichiro Sekiya. Not single
but periodic injections of synovial mesenchymal stem cells maintain viable cells in knees and inhibit
osteoarthritis progression in rats. Osteoarthr. Cartil.. 2016.06; 24(6); 1061-1070
12. Hideyuki Koga, Takeshi Muneta. Graft impingement ACL Injury and Its Treatment. 2016.06; 267-278
13. Hideyuki Koga, Takeshi Muneta. ACL injury mechanisms ACL Injury and Its Treatment. 2016.06;
113-128
14. Takeshi Muneta, Hideyuki Koga. Part X Revision ACL Reconstruction. 36 Double-Bundle Technique.
ACL Injury and Its Treatment, Ochi M, Shino K, Yasuda K, Kurosaka M, Eds.. 2016.06; 453-468
15. Mio Udo, Takeshi Muneta, Kunikazu Tsuji, Nobutake Ozeki, Yusuke Nakagawa, Toshiyuki Ohara, Ryusuke
Saito, Katsuaki Yanagisawa, Hideyuki Koga, Ichiro Sekiya. Monoiodoacetic acid induces arthritis and synovitis in rats in a dose- and time-dependent manner: proposed model-speciﬁc scoring systems. Osteoarthr.
Cartil.. 2016.07; 24(7); 1284-1291
16. Takeshi Muneta, Hideyuki Koga, Tomomasa Nakamura, Masafumi Horie, Toshifumi Watanabe, Ichiro
Sekiya. Behind-remnant arthroscopic observation and scoring of femoral attachment of injured anterior
cruciate ligament. Knee Surg Sports Traumatol Arthrosc. 2016.09; 24(9); 2906-2914― 100 ―

Bio-Matrix

Bio-Matrix

17. Hideyuki Koga, Takeshi Muneta, Toshifumi Watanabe, Tomoyuki Mochizuki, Masafumi Horie, Tomomasa
Nakamura, Koji Otabe, Yusuke Nakagawa, Ichiro Sekiya. Two-Year Outcomes After Arthroscopic Lateral
Meniscus Centralization. Arthroscopy. 2016.10; 32(10); 2000-2008
18. Arata Yuuki, Takeshi Muneta, Toshiyuki Ohara, Ichiro Sekiya, Hideyuki Koga. Associated lateral/medialknee instability and its relevant factors in anterior cruciate ligament-injured knees. J Orthop Sci. 2016.11;
19. Toshifumi Watanabe, Takeshi Muneta, Hideyuki Koga, Masafumi Horie, Tomomasa Nakamura, Koji
Otabe, Yusuke Nakagawa, Mai Katakura, Ichiro Sekiya. In-vivo kinematics of high-ﬂex posterior-stabilized
total knee prosthesis designed for Asian populations. Int Orthop. 2016.11; 40(11); 2295-2302
20. Kaori Nakamura, Hideyuki Koga, Ichiro Sekiya, Toshifumi Watanabe, Tomoyuki Mochizuki, Masafumi
Horie, Tomomasa Nakamura, Koji Otabe, Takeshi Muneta. Dynamic Evaluation of Pivot-Shift Phenomenon in Double-Bundle Anterior Cruciate Ligament Reconstruction Using Triaxial Accelerometer.
Arthroscopy. 2016.12; 32(12); 2532-2538
21. Toshiyuki Ohara, Takeshi Muneta, Yusuke Nakagawa, Yu Matsukura, Shizuko Ichinose, Hideyuki Koga,
Kunikazu Tsuji, Ichiro Sekiya. < Original Article> Hypoxia enhances proliferation through increase ofcolony formation rate with chondrogenic potential in primary synovial mesenchymal stem cells. J. Med.
Dent. Sci.. 2016.12; 63(4); 61-70[Conference Activities & Talks]
1. Gaku Koyano, Tetsuya Jinno, Daisuke Koga, Takeshi Muneta, Atsushi Okawa. Bilateral Comparison of
Hip Joint Morphology between the Hips with and without Herniation Pit. Orthopaedic Research Society
2016.03
2. Hideyuki Koga,Takeshi Muneta, Ichiro Sekiya. Clinical Outcomes after Arthroscopic Centralization of an
Extruded Lateral Meniscus. AAOS 2016.03.02
3. Toshiyuki Ohara,Takeshi Muneta, Yusuke Nakagawa, Yu Matsukura, Kunikazu Tsuji, Ichiro Sekiya. Hypoxia enhances proliferation with chondrogenic potential in primary synovial MSCs. Orthopaedic ResearchSociety 2016 Annual Meeting 2016.03.05
4. Yusuke Nakagawa, Hideyuki Koga, Takeshi Muneta, Kiuchi S, Ono H, Nagata T, Toshifumi Watanabe,
Masafumi Horie, Tomomasa Nakamura, Koji Otabe, Mai Katakura, Hisako Katano, Ichiro Sekiya. Threedimensional Reconstructed MRI Evaluation of Arthroscopic Centralization Technique for Extruded Lateral
Meniscus. Orthopaedic Research Society 2016 Annual Meeting 2016.03.05
5. Toshifumi Watanabe,Takeshi Muneta, Ichiro Sekiya, Hideyuki Koga, Masafumi Horie, Tomomasa Nakamura, Koji Otabe, Scott Banks. In-vivo Kinematics of Posterior-stabilized Total Knee Prosthesis Designed
for Asian. Orthopaedic Research Society 2016 Annual Meeting 2016.03.05
6. Kaori Nakamrua, Kunikazu Tsuji, Hiroki Katagiri, Makiko Inoue, Miyoko Ojima, Ichiro Sekiya, Takeshi
Muneta. Dynamic analysis of inﬂammatory cells in synovial ﬂuid after index anterior cruciate ligament
reconstruction surgery. Osteoarthritis Research Society 2016 Annual Meeting 2016.03.05
7. Etsuko Matsumura, Takeshi Muneta, Kunikazu Tsuji, Keiichiro Komori, Ichiro Sekiya. Il-1 β Enhances
Proliferation And Chondrogenic Potential Of Synovial MSCs. Orthopaedic Research Society 2016 Annual
Meeting 2016.03.05 Orlando,USA
8. Nobutake Ozeki, Junpei Matsuda, Takeshi Muneta, Hideyuki Koga, Kunikazu Tsuji, Kenta Katagiri,
Mitsuru Mizuno, T Saito, Ichiro Sekiya. Biomechanical analysis of centralization with an anchor formeniscus extrusion in a porcine model. Orthopaedic Research Society 2016 Annual Meeting 2016.03.05
Orlando, USA
9. Yuji Kohno, Mitsuru Mizuno, Kenta Katagiri, Koji Otabe, Nobutake Ozeki, Masafumi Horie, Hideyuki
Koga, Kunikazu Tsuji, Mikio Matsumoto, Haruka Kaneko, Yuji Takazawa, Takeshi Muneta, Ichiro Sekiya.
Yields and chondrogenic potential of synovial MSCs are comparable between RA and OA patients who
undergo TKA. Orthopaedic Research Society 2016 Annual Meeting 2016.03.05― 101 ―

Bio-Matrix

Bio-Matrix

10. Mitsuru Mizuno, Hisako Katano, Koji Otabe, Keiichiro Komori, Yukie Matsumoto,Nobutake Ozeki, Kunikazu Tsuji, Takeshi Muneta, Ichiro Sekiya. PDGF-AA/AB In Human Serum Are Potential Indicators Of
The Proliferative Capacity Of Human Synovial Mesenchymal Stem Cells. Orthopaedic Research Society
2016 Annual Meeting 2016.03.06 Orlando, USA
11. Mitsuru Mizuno, Hisako Katano, Yo Mabuchi, Yusuke Ogata, Shizuko Ichinose, Koji Otabe, Keiichiro
Komori, Yukie Matsumoto, Nobutake Ozeki, Kunikazu Tsuji, Chihiro Akazawa, Takeshi Muneta, Ichiro
Sekiya. Properties of MSCs Derived From Surface, Stroma, and Perivascular Synovium Of Oa Patients.
Orthopaedic Research Society 2016 Annual Meeting 2016.03.06 Orlando, USA
12. Mana Naritomi, Mitsuru Mizuno, Hisako Katano, Koji Otabe, Nobutake Ozeki, Kunikazu Tsuji, Takeshi
Muneta, Ichiro Sekiya. Interleukin-1 β Promotes In Vitro Chondrogenesis Of Synovial Mesenchymal StemCells. Orthopaedic Research Society 2016 Annual Meeting 2016.03.06 Orlando, USA
13. Hideyuki Koga,Kaori Nakamura, Ichiro Sekiya, Takeshi Muneta. Minimum required tension in doublebundle anterior cruciate ligament reconstruction based on objective evaluation of pivot shift phenomenonusing triaxial accelerometer. ACL Study Group Meeting 2016.03.13
14. Miyoko Ojima, Kunikazu Tstuji, Koji Otabe, Masafumi Horie, Hideyuki Koga, Ichiro Sekiya, Takeshi
Muneta. Diﬀerent methods of detaching adherent cells signiﬁcantly aﬀect the detection of stem cell
antigens in synovial mesenchymal stem cells.. OARSI 2016 World Congress on Osteoarthritis 2016.03.31
15. Hideyuki Koga. Keynote lecture: ACL injury - video analysis. 17th ESSKA 2016.05.04 Barcelona, Spain
16. Hideyuki Koga, Takeshi Muneta, Ichiro Sekiya. Clinical and Radiographic Outcomes after Arthroscopic
Centralization of Lateral Meniscus Extrusion. 17th ESSKA 2016.05.04 Barcelona, Spain
17. Toshifumi Watanabe, Takeshi Muneta, Kenji Hara, Hideyuki Koga, Masafumi Horie, Tomomasa Nakamura, Koji Otabe, Yusuke Nakagawa, Mai Katakura, Atsushi Okawa, Ichiro Sekiya. Is Closed Suction
Drainage Necessary for Total Knee Arthroplasty? :A Prospective Study of a Mean Follow-up of 5.5 Years.
89th Annual Meeting of JOA 2016.05.12 Yokohama, Japan
18. Hideyuki Koga, Takeshi Muneta, Toshifumi Watanabe, Tomoyuki Mochizuki, Masafumi Horie, Tomomasa
Nakamura, Koji Otabe, Yusuke Nakagawas, Mai Katakura, Atsushi Okawa, Ichiro Sekiya. Two-year
results of remnant-preserved double-bundle anterior cruciate ligament reconstruction: Frontside approachversus behind-remnant approach. 89th JOA 2016.05.12 Yokohama, Japan
19. Hideyuki Koga, Takeshi Muneta, Toshifumi Watanabe, Tomoyuki Mochizuki, Masafumi Horie, Tomomasa
Nakamura, Koji Otabe, Yusuke Nakagawas, Mai Katakura, Atsushi Okawa, Ichiro Sekiya. Clinical outcomes after arthroscopic centralization of lateral meniscus extrusion. 89th JOA 2016.05.12 Yokohama,Japan
20. Mari Uomizu, Hideya Yoshimura, Akimoto Nimura, Takashi Miyamoto, Tomoyuki Mochizuki. Return toSports Activity after Arthroscopic Rotator Cuﬀ Repair on the Middle-aged and Senior Patients. The 13th
International Congress of Shoulder and Elbow Surgery 2016.05.18 Korea
21. Ryohei Takada, Tetsuya Jinno, Daisuke Koga, Masanobu Hirao, Takeshi Muneta, Atsushi Okawa. Comparison of wear rate and osteolysis between annealed and remelted highly cross-linked polyethylene intotal hip arthroplasty. 17th EFORT 2016.06
22. Ryohei Takada, Tetsuya Jinno, Daisuke Koga, Masanobu Hirao, Takeshi Muneta, Atsushi Okawa. Limited
signiﬁcance of screening computed tomography after cementless total hip arthroplasty with highly crosslinked polyethylene. 17th EFORT 2016.06
23. Hideyuki Koga. Arthroscopic Centralization of an Extruded Lateral Meniscus. JOSKAS-SIGASCOT
travelling fellowship 2016.09.20 Parma, Italy
24. Hideyuki Koga. Arthroscopic Centralization of an Extruded Lateral Meniscus. JOSKAS-SIGASCOT
travelling fellowship 2016.09.22 Milan, Italy
25. Etsuko Matsumura, Kunikazu Tsuji, Keiichiro Komori, Hideyuki Koga, Ichiro Sekiya，Takeshi Muneta.
nvestigate the eﬀects of pretreatment of IL-1 β on proliferation and chondrogenic diﬀerentiation of synovial
MSCs. ICRS 2016 2016.09.24― 102 ―

Bio-Matrix

Bio-Matrix

26. Miyoko Ojima, Kunikazu Tstuji, Koji Otabe, Masafumi Horie, Hideyuki Koga, Ichiro Sekiya, Takeshi
Muneta. Analyses of biologically active substances in synovial ﬂuid which support proliferation of synovial
mesenchymal stem cells. International Cartilage Repair Society 2016 Annual Meeting 2016.09.24
27. Hideyuki Koga, Takeshi Muneta, Ichiro Sekiya. Two-year outcomes after arthroscopic centralization of an
extruded lateral meniscus. 6th SIGASCOT 2016.09.28 Florence, Italy
28. Hideyuki Koga. Arthroscopic Centralization of an Extruded Lateral Meniscus. JOSKAS-SIGASCOT
travelling fellowship 2016.10.03 Rome, Italy
29. Hideyuki Koga. Minimum required tension in double-bundle ACL reconstruction based on objective evaluation of pivot shift phenomenon using triaxial accelerometer. JOSKAS-SIGASCOT travelling fellowship2016.10.03 Rome, Italy
30. Hideyuki Koga. Minimum required tension in double-bundle ACL reconstruction based on objective evaluation of pivot shift phenomenon using triaxial accelerometer. JOSKAS-SIGASCOT travelling fellowship2016.10.05 Rome, Italy― 103 ―

Bio-Matrix

Bio-Matrix

Biostructural Science

Associate Professor: Makoto TABATA
Technician: Makoto SUGIURA
Secretary: Tomoko YAMAMOTO
Graduate Student: Takafumi NAKANO(1)

OutlineSection of biostructural science is the inheritor of the laboratory of Oral Anatomy II, then our focus is understanding of the mechanism and histology of tooth formation. And also we study the tooth evolution using ﬁshscale and tooth.
(2)

Research

The study of the mechanisms of dental formation and their evolution is the central focus of our research.
Followings are rough description of current research subjects in our laboratory.
1) Study of the mechanism in tooth development
2) Study of the mechanism in ameloblast diﬀerentiation
3) Histological and developmental study of ﬁsh scales and teeth4) Space experiments for the bone biology using ﬁsh(3)

Lectures & Courses

We are inheritor of the laboratory of Oral Anatomy II, then we involved in the education of histology, embryology,
and oral histology.
In the ﬁrst place, anatomy and histology is the study to learn the structure, the name, and the function of"HUMAN BODY". Then the subject histology is not able to separate from subject anatomy, relate to physiology,
pathology, and embryology and further become to be the fundamentals of clinical subjects. So we carry out of
our subjects, with an awareness of the relationships between histology and other subjects.
On the curriculum of the 2nd grade of dental students, lectures of histology contains practical histology using
tissue sections and microscopy. This skills work is a good opportunity to know the variation and the ﬁnesse of
the human body in histology.

― 104 ―

Bio-Matrix
Bio-Matrix

Pharmacology

Staﬀs and Students(April, 2016)
Associate Professor Kazuhiro AOKIAssistant Professor Yukihiko TAMURA
Technologist Mariko TAKAHASHI
Researchers
Nobuyoshi TOMOMATSU (Maxillofacial Surgery)
Yasuhiro SHIMIZU (Orthodontic Science)
Tomoki UEHARA (Pediatric Dentistry)
Yuki ARAI (Removable Prosthodontics)
Graduate Students
Md. Haque Bhuyan ZAHIRUL
Toru TAKEMOTO(Oral and Maxillofacial Surgery)
Kenya YONEDA(Regenerative Dental Medicine)
Hideaki INAGAWA(Removal Prosthodontics)
Eri SONE(Maxillofacial Surgery)
Hisami OKAWARA(Removable Prosthodontics)
Michiko OZAWA
Lecturers
Etsuko TAKAHASHI
Akira NIFUJI
Nozomi HASEGAWA
Eiichi MURASE
Yoshihiro WAKI
Hiroyuki SETO
Toshimi SATO
Genki KATO
Setsuko MISE
Kiichi NONAKA(1)

ResearchResearch subjects
1) Pharmacological analyses of formation and resorption on bones and teeth
2) Identiﬁcation of a new therapeutic target for hard tissue-related diseases
3) Translational research for hard tissue regeneration
4) Interdisciplinary research toward the application of peptide drug5) Analyses of drug side eﬀects appeared at oral tissues― 105 ―

Bio-Matrix
Bio-Matrix(2)

Lectures & Courses

Purpose of Education
Pharmacology is situated between the basic and clinical sciences and is important for dental students. There isa growing demand on the dental clinicians to know huge knowledge of drugs and how to use them for patients.
For these purpose, the ﬁrst lecture is aimed to teach the scientiﬁc aspects of pharmacology and how drugs act
on the various organ system. The second lecture deals with drugs of medical and dental ﬁelds and the last withdrugs of special importance of dentistry. Dental students learn the principle of pharmacology through laboratory
practice. Following these learning, they must acquire an adequate background for drug use in general practice.

(3)

Publications[Original Articles]
1. Trang N Nguyen Vo, Jia Hao, Josh Chou, Masamitsu Oshima, Kazuhiro Aoki, Shinji Kuroda, Boosana
Kaboosaya, Shohei Kasugai. Ligature induced peri-implantitis: tissue destruction and inﬂammatory
progression in a murine model. Clin Oral Implants Res. 2016.01;
2. T Uehara, S Mise-Omata, M Matsui, Y Tabata, R Murali, M Miyashin, K Aoki. Delivery of RANKLBinding Peptide OP3-4 Promotes BMP-2-Induced Maxillary Bone Regeneration. J. Dent. Res.. 2016.03;
3. Moe Htet, Marwa Madi, Ossama Zakaria, Takayuki Miyahara, Wang Xin, Zayar Lin, Kazuhiro Aoki,
Shohei Kasugai. Decontamination of Anodized Implant Surface with Diﬀerent Modalities for Peri-Implantitis
Treatment: Lasers and Mechanical Debridement with Citric Acid. J. Periodontol.. 2016.03; 1-17
4. Yuki Arai, Kazuhiro Aoki, Yasuhiro Shimizu, Yasuhiko Tabata, Takashi Ono, Ramachandran Murali, Setsuko Mise-Omata, Noriyuki Wakabayashi. Peptide-induced de novo bone formation after tooth extraction
prevents alveolar bone loss in a murine tooth extraction model. Eur. J. Pharmacol.. 2016.07; 782; 89-97
5. Yasutaka Sugamori, Setsuko Mise-Omata, Chizuko Maeda, Shigeki Aoki, Yasuhiko Tabata, Ramachandran Murali, Hisataka Yasuda, Nobuyuki Udagawa, Hiroshi Suzuki, Masashi Honma, Kazuhiro Aoki.
Peptide drugs accelerate BMP-2-induced calvarial bone regeneration and stimulate osteoblast diﬀerentiation through mTORC1 signaling. Bioessays. 2016.08; 38(8); 717-725[Conference Activities & Talks]
1. Yukihiko Tamura, Setsuko Mise, Yasutaka Sugamori, Md. Zahirul Haq Bhuyan, Mariko Takahashi,
Tomoki Uehara, Yuki Arai, Michiyo Miyashin, Noriyuki Wakabayashi, Kazuhiro Aoki. A pharmacological role of metallothionein in zinc-treated cartilage-progenitor cells. APFP 2016(The 13th Asia Paciﬁc
Federation of Pharmacologists Meeting) 2016.02.01 The Berkeley Hotel Pratunam,Bangkok (Thailand)
2. Mikami R, Aoki A, Mizutani K, Tamura Y, Aoki K, Izumi Y. Low-level laser irradiation using a highfrequency ultra-short pulsed blue laser enhances osteoblasts proliferation and diﬀerentiation. The 59th
Spring Meeting of The Japanese Society of Periodontology 2016.05.20 Kagoshima
3. Md.Zahirul Haque Bhuyan, Chizuko Maeda, Yuki Yoshinari, Marin Kawasaki, Yasutaka Sugamori, Genki
Kato, Mariko Takahashi, Yukihiko Tamura, Kazuhiro Aoki. The therapeutic eﬀects of the RANKLbinding peptides on cartilage destruction.. 13th Bone Biology Forum 2016.08.20 Cross Wave Makuhari(Chiba)4. Mikami R, Aoki A, Mizutani K, Tamura Y, Aoki K, Izumi Y. Low-level blue laser irradiation enhancesosteoblast proliferation and diﬀerentiation. The 102nd Annual Meeting of the American Academy of
Periodontology 2016.09.10 San Diego, USA
5. Mikami R, Aoki A, Mizutani K, Tamura Y, Aoki K, Izumi Y. Low-level blue laser irradiation enhancesosteoblast proliferation and diﬀerentiation. The 102nd Annual Meeting of the American Academy of
Periodontology 2016.09.10 San Diego Convention Center(USA)― 106 ―

Bio-Matrix
Bio-Matrix

Connective Tissue Regeneration

Associate Professor Tamayuki SHINOMURA(1)OutlineOur group is interested in the restoration of damaged connective tissue. In general, connective tissue is characterized by the presence of abundant extracellular matrix, and its function is highly dependent on the propertiesof extracellular matrix. Therefore, to restore connective tissue normal, it is essential for us to understand the
behavior of extracellular matrix molecules. So, we pursue research on the molecular mechanisms underlying theformation and maintenance of extracellular matrix in connective tissues.
(2)

Research

Currently, we are engaged in the following research subject.
1. Study on the mechanisms that regulate the expression of type II collagen and aggrecan in chondrocytes.
2. Study on transcription factors necessary for the maintenance of chondrogenic phenotype.
3. Study on the molecular dynamics of extracellular matrix in connective tissues.

(3)

Education

We give a lecture on molecular biology in general and laboratory exercise for undergraduate students. Also,in our graduate course, we oﬀer lectures on the synthesis and formation of extracellular matrix focusing on
cartilage tissue.

(4)

Lectures & Courses

We provide education based on the belief that an integrated and organized connection of various knowledge isimportant to understand life phenomena.
(5)

Publications[Original Articles]
1. K, Ito, T. Shinomura. Development and application of a new silent reporter system to quantitate theactivity of enhancer elements in the type II collagen gene Gene. 2016.03; 585; 13-21
2. Tani, H., Sato, Y., Ueda, M., Miyazaki, Y., Suginami, K., Horie, A., Konishi, I., and Shinomura, T..
Role of Versican in the Pathogenesis of Peritoneal Endometriosis The Journal of Clinical Endocrinology& Metabolism. 2016.11; 101(11); 4349-4356― 107 ―

Bio-Matrix
Bio-Matrix

3. Nakayama Y, Kobayashi R, Matsui S, Matsumura H, Iwai Y, Noda K, Yamazaki M, Kurita-Ochiai T,
Yoshimura A, Shinomura T, Ganss B, Ogata Y.. Localization and expression pattern of amelotin, odontogenic ameloblast-associated protein and follicular dendritic cell-secreted protein in the junctional epithelium of inﬂamed gingiva Odontology. 2016.11;― 108 ―

Bio-Matrix
Bio-Matrix

Biochemistry

Professor Testuro Watabe
Associate Professor Miki Yokoyama
Junior Associate Professor Yasuhiro Kumei
Assistant Professor Katarzyna Anna Podyma-Inoue
Technical staﬀ Kazue Terasawa
Part-time Lecturer Zeredo, Jorge Luis Lopes, Akira Asari
Graduate student Akihiko Inagawa, Takumi Matsuda
Postgraduate Rajapakshe Mudiyanselage Anupama Rasadari Rajapakshe(1)OutlineSince cancer is the leading cause of death in Japan, we need to develop novel strategies to cure it. Tumor
consists of not only cancer cells but also the non-cancerous cells including ﬁbroblasts, immune cells and cells
that comprise the blood and lymphatic vessels. We aim to elucidate the mechanisms how cancer cells become
malignant by the various cytokines in cancer microenvironment in order to develop novel therapeutic strategies
targeting multiple components of cancer microenvironment.
(2)

Research(1) Understanding the molecular mechanisms underlying endothelial-mesenchymal transition (EndMT)Endothelial cells undergo diﬀerentiation into mesenchymal cells during not only various physiological processes
including heart valve formation but also pathological processes including cancer progression, heart failure anddiabetes. However, the molecular mechanisms that regulate such endothelial-mesenchymal transition (EndMT)
remain to be elucidated. We aim to study the molecular mechanisms underlying EndMT in order to identify
novel targets and attempt to develop therapeutic strategies for EndMT-related diseases.
(2) Elucidation of the molecular mechanisms underlying tumor angio- and lympangiognesis
Tumor angiogenesis and lymphangiogenesis are key features of tumor progression and metastasis. While multiple signaling pathways have been implicated in the formation of blood and lymphatic vessels, the molecularmechanisms underlying these processes have not yet fully elucidated. Recent ﬁndings revealed that membersof the transforming growth factor-β (TGF-β) family play pivotal roles on in angiogenesis and lymphangiogenesis, and that abnormalities in TGF-β family signaling lead to development of certain vascular disorders,
including hereditary hemorrhagic telangiectasia (HHT), pulmonary arterial hypertension, Marfan syndrome andLoeys-Dietz syndrome. We attempt to elucidate the molecular mechanisms how TGF-β family signals regulate
antiogenesis and lymphaniogenesis in tumor microenvironment.
(3) Understanding the molecular mechanisms underlying metastasis of cancer cells
Epithelial-mesenchymal transition (EMT) plays important roles in various physiological and pathological processes, and is regulated by signaling pathways mediated by cytokines including TGF- β. Using various typesof in vitro cultured oral carcinoma cells and in vivo systems, we aim to identify the molecules involved in theacquisition of invasive properties of cancer cells, in order to develop novel therapeutic strategies.
(4) Structural and functional mapping of lysosomal membranes
Lysosomes are ubiquitous organelles rich in hydrolytic enzymes, responsible for the degradation of macro-― 109 ―

Bio-Matrix
Bio-Matrix

molecules derived from the extracellular space through endocytosis or phagocytosis, and from the cytoplasm
through autophagy. These processes are vital to acquire energy source and essential materials, to defend against
pathogen, and to clear unnecessary or potentially harmful intracellular obstacles. Furthermore, accumulating
evidence has revealed that the lysosome surface is a crucial site for the activation of mammalian or mechanistic
target of rapamycin complex 1 (mTORC1), a master regulator of cell growth and metabolism. mTORC1. Clinically, impairment of lysosome function gives rise to progressive and ultimately serious damages in widespreadtissue and organ, particularly in brain, bones and connective tissues.
Lysosome-associated membrane proteins 1 and 2 (LAMP-1 and LAMP-2) have a large, heavily glycosylated
luminal domain composed of the two subdomains, and are the most abundant protein components in lysosome
membranes. LAMP-1 and LAMP-2 have distinct functions, and the presence of both proteins together is required for the vital essential regulation of autophagy to avoid embryonic lethality. However, the structural
aspects of LAMP-1 and LAMP-2 haves not been elucidated. Here, we demonstrated that the subdomains of
LAMP-1 and LAMP-2 have adopt the unique β-prism fold. Furthermore, we found that the N-domain of the
LAMP-1 is necessary, whereas that of LAMP-2 is repressive, for the organization of a multimeric assembly of
the LAMPs. Accordingly, the present study suggests for the ﬁrst time that the assembly modes of LAMP-1
and LAMP-2 are diﬀerent, which may underlie their distinct functions [ Biochemical and Biophysical ResearchCommunications 479, 489-495 (2016)] .
(5) Heparan sulfate proteoglycan-dependent cellular logistics
Heparan sulfate proteoglycans (HSPGs) are one of the basic constituents of plasma membranes and have ability
to interact with a number of the extracellular ligands. HSPGs have been suggested to mediate the traﬃcking ofvarious macromolecules from the cell surface. Growth factors, cytokines, lipoproteins, cell penetrating peptides,
polycation-nucleic acid complexes, along with exosomes, and pathogens enter cells through HSPG-dependentendocytosis. HSPGs-dependent endocytic events have been involved in tumor progression, stressing the importance of the identiﬁcation of HSPG species participating in a formation of various endocytic complexes. We
have characterized the intracellular traﬃcking complexes formed in the presence of HSPGs in a rat C6 glioma
cell line model. Successful isolation of HSPG-positive transport vesicles followed by detailed proteomic analysis
allowed us the identiﬁcation of over eighty proteins related to vesicular transport; i.e. endocytosis or recycling.
Part of HSPGs in glioma cells found to be internalized through clathrin-dependent endocytosis and underwent
recycling. Further characterization of HSPG-rich vesicular compartments will help us understand the natureof HSPG-ligand interactions and to design the tools for targeted delivery of ligands into the cells (reported inCancer Genomics & Proteomics, 2016, 13, 443-452).
(6) Posture, behavior, and motion sickness of common marmosets in low gravities.
In planetary development projects such as manned Mars exploration that will take longer than 3 years, longterm biological adaptation to weightlessness and partial-gravity environments is a critical issue. Since rodents
of short lifespan (rats and mice) cannot survive in such a long-term ﬂight, other animals of longer lifespanmust be used alternatively so as to conduct appropriate studies on posture and exploration as well as voicecommunication and social behaviors of animals that have some relevance to long-term manned spaceﬂight.
Common marmoset belonging to the same anthropoid with humans has a lifespan of 15 years is characterized
by unique social behavior resembling humans with abundant squealing. In parabolic ﬂight experiment carriedout in February 2016, we ﬁrst examined the adaptation and social behavior of common marmoset under suchconditions as Moon and Mars simulation or weightlessness. We have gained new knowledge on the response
and behavior of primates in partial gravities.

(3)

Education

For the second-year undergraduate students, we are in charge of the unit, Molecular aspect of cell biology
and Laboratory course under the module of Molecular basis of biology . The contents of Molecular
aspect of cell biology (lecture) includes, topics related to the structure and function of membranes, transport
across membranes, organization and function of intracellular organelles, intracellular traﬃcking, cytoskeleton,
extracellular matrix, signal transduction, cell cycle and cell death. During the laboratory course, the puriﬁcation and characterization of an enzyme (pH-dependency, eﬀects of inhibitors, comparison of isozymes, kinetic
analysis) were done.
For the graduate students, in order to demonstrate various research examples, we lectured on the following― 110 ―

Bio-Matrix
Bio-Matrix

subjects; (1) cell-surface assembly of proteins (CD38 as an example), (2) membrane domains (lipid rafts) andsphingosine-1-phosphate signaling, (3) structure and function of proteoglycans, (4) structure and role of extracellular matrix (5) analysis of the response to the gravity change at a cellular and individual levels and its
clinical application. All the lectures were done in English. We also hold the special lectures "Tissue Myeloid
Progenitors Diﬀerentiate into Pericytes in Developing Skin Vasculature" (October 14, 2016) and New functionof lysosomes in osteoclasts and osteoblasts (December 22, 2016).
(4)

Lectures & Courses

For the undergraduate students, our aim is to provide the students with the basic knowledge in biochemistry tohelp them to understand cellular function based on the structure and function of biomolecules. For the graduate
students, we encourage them to acquire an ability and research skill to study the cellular responses at molecularlevels.
(5)

Publications[Original Articles]
1. Masato Morikawa, Daizo Koinuma, Anna Mizutani, Natsumi Kawasaki, Katarina Holmborn, Anders
Sundqvist, Shuichi Tsutsumi, Tetsuro Watabe, Hiroyuki Aburatani, Carl-Henrik Heldin, Kohei Miyazono.
BMP Sustains Embryonic Stem Cell Self-Renewal through Distinct Functions of Diﬀerent Krüppel-like
Factors. Stem Cell Reports. 2016.01; 6(1); 64-73
2. Akihiro Katsura, Hiroshi I Suzuki, Toshihide Ueno, Hajime Mihira, Tomoko Yamazaki, Takahiko Yasuda, Tetsuro Watabe, Hiroyuki Mano, Yoshitsugu Yamada, Kohei Miyazono. MicroRNA-31 is a positive
modulator of endothelial-mesenchymal transition and associated secretory phenotype induced by TGFβ. Genes Cells. 2016.01; 21(1); 99-116
3. Fusa Ogata, Katsuhito Fujiu, Sahohime Matsumoto, Yukiteru Nakayama, Munehiko Shibata, Yuichi Oike,
Isao Koshima, Tetsuro Watabe, Ryozo Nagai, Ichiro Manabe. Excess Lymphangiogenesis Cooperatively
Induced by Macrophages and CD4(+) T Cells Drives the Pathogenesis of Lymphedema. J. Invest. Dermatol.. 2016.03; 136(3); 706-714
4. Kyohei Muguruma, Fumika Yakushiji, Ryosuke Kawamata, Daichi Akiyama, Risako Arima, Takuya Shirasaka, Yamato Kikkawa, Akihiro Taguchi, Kentaro Takayama, Takeshi Fukuhara, Tetsuro Watabe, Yuji
Ito, Yoshio Hayashi. Novel Hybrid Compound of a Plinabulin Prodrug with an IgG Binding Peptide forGenerating a Tumor Selective Noncovalent-Type Antibody-Drug Conjugate. Bioconjug. Chem.. 2016.07;
27(7); 1606-1613
5. Kazue Terasawa, Yuri Tomabechi, Mariko Ikeda, Haruhiko Ehara, Mutsuko Kukimoto-Niino, Motoaki
Wakiyama, Katarzyna A Podyma-Inoue, Anupama R Rajapakshe, Tetsuro Watabe, Mikako Shirouzu,
Miki Hara-Yokoyama. Lysosome-associated membrane proteins-1 and -2 (LAMP-1 and LAMP-2) assemble
via distinct modes. Biochem. Biophys. Res. Commun.. 2016.10; 479(3); 489-495
6. Katarzyna A Podyma-Inoue, Takuya Moriwaki, Anupama R Rajapakshe, Kazue Terasawa, Miki HaraYokoyama. Characterization of Heparan Sulfate Proteoglycan-positive Recycling Endosomes Isolated from
Glioma Cells. Cancer Genomics Proteomics. 2016.12; 13(6); 443-452
7. Y Kumei, R Shimokawa, M Kimoto, Y Kawauchi, H Shimokawa, K Makita, K Ohya, K Toda. Gravity
stress elevates the nociceptive threshold level with immunohistochemical changes in the rat brain. Acta
Astronaut. 49(3-10); 381-390[Misc]
1. Yasuhiro Yoshimatsu, Hideki Miyazaki, Tetsuro Watabe. Roles of signaling and transcriptional networks
in pathological lymphangiogenesis. Adv. Drug Deliv. Rev.. 2016.04; 99(Pt B); 161-171
2. Takenobu Katagiri, Tetsuro Watabe. Bone Morphogenetic Proteins. Cold Spring Harb Perspect Biol.
2016.06; 8(6);― 111 ―

Bio-Matrix
Bio-Matrix

3. Yasuhiro Yoshimatsu. Roles of Transcription Factors in the Formation of Lymphatic Vessels Inﬂammation
and Immunity. 2016.08; 24(5); 372-378
4. Tetsuro Watabe. Towards understanding cancer microenvironment Journal of the Stomatological Society.
2016.11; 83(3); 103-106[Conference Activities & Talks]
1. Tetsuro Watabe, Yuichi Akatsu, Yasuhiro Yoshimatsu, Kohei Miyazono. Dual targeting of vascular endothelial growth factor and bone morphogenetic protein-9/10 impairs tumor growth through inhibition ofangiogenesis. 10th AACR-JCA Joint Conference 2016.02.17
2. Tetsuro Watabe. Roles of TGF-ß signals in endothelial-to-mesenchymal transition (EndMT) of lymphatic
endothelial cells. Gordon Research Conference Lymphatics 2016.03.23 Ventura, USA
3. Ershu Ren, Ippei Watari, Jui-Chin Hsu, Rieko Ono, Emina Wakasugi-Aoyama, Mariko MizumachiKubono, Katarzyna A. Podyma-Inoue, Tetsuro Watabe, Takashi Ono. Unilateral nasal obstruction affects taste cells in circumvallate papillae in rat. The 17th International symposium on olfaction and taste(ISOT2016) 2016.05 Yokohama, Japan
4. Beauboeuf Roody, Ippei Watari, Jui-Chin Hsu, Mariko Mizumachi-Kubono, Katarzyna A. Podyma-Inoue,
Tetsuro Watabe, Takashi Ono. Expression of GLP-1 and GLP-1 receptor in rat circumvallate papillae
during growth period. The 17th International symposium on olfaction and taste (ISOT2016) 2016.05
Yokohama, Japan
5. Anupama R. Rajapakshe, Katarzyna A. Podyma-Inoue, Kazue Terasawa, and Miki Hara-Yokoyama.
Lysosome-associated membrane proteins (LAMPs) regulate intracellular positioning of mitochondria in
MC3T3-E1 cells. The Pathobiology of the Lysosome and Lysosomal Diseases Conference 2016.07 Cambridge, England
6. Katarzyna A. Podyma-Inoue, Miki Yokoyama and Tetsuro Watabe. Characterization of extracellular
vesicles isolated from osteoblastic cells lines. The 89th Annual Meeting of Japanese Biochemistry Society
2016.09 Sendai, Japan
7. Tetsuro Watabe. Roles of signaling networks during endothelial-to-mesenchymal transition (EndMT) in
cancer microenvironment.. 2016.10.08
8. Yasuhiro Kumei, Jorge Zeredo, Ryota Watahiki, Machiko Hattori, Hitoshi Hibino, Kensuke Kagiyama,
Kazumasa Fukasawa, Hirofumi Hashimoto, Noriaki Ishioka, Yuko Inatomi . Motion Sickness of Common Marmosets in Lunar and Martian Gravity Environments by Parabolic Flight. American Society for
Gravitational and Space Research 2016.10.27
9. Tetsuro Watabe. Dual targeting of vascular endothelial growth factor and bone morphogenetic protein9/10 impairs tumor growth through inhibition of angiogenesis. 11th International BMP Conference
2016.10.28 Boston
10. Ryota Watahiki, Jorge Zeredo, Kensuke Kagiyama, Hirofumi Hashimoto, Noriaki Ishioka, Yuko Inatomi,
Otto Baba, Shuji Aou, Kiyohisa Natsume, Yasuhiro Kumei. Postural and Behavioral Response of Primates (Common Marmoset) to Martian, Lunar, and Zero Gravity Conditions. American Society for
Gravitational and Space Research 2016.10.29
11. Kazuki Takahashi, Katarzyna Anna Inoue,Yasuhiro Yoshimatsu. Hironori Harada and Tetsuro Watabe.
Role of TGF-β in the invasiveness of oral cancer cell lines. The 39th Annual Meeting of Molecular Biology
Society of Japan 2016.12 Yokohama
12. Yasuhiro Yoshimatsu. Roles of signaling and transcriptional networks in the formation and maintenanceof lymphatic vasculature. The 24th Annual Meeting of the Japanese Vascular Biology and Medicine
Organization/ The 14th Korea-Japan Joint Symposium on Vascular Biology 2016.12.10 Nagasaki― 112 ―

Bio-Matrix
Bio-Matrix

Cell Signaling

Professor(Principal Investigator)Tomoki NAKASHIMA
Assistant Professor Mikihito HAYASHI
Assistant Professor Takehito ONO(1)ResearchResearch Subjects1)Regulation of bone remodeling by bone cells2)Identiﬁcation of bone-derived systemic regulatory factors (osteokines)
3)Mechanism of sensing and adapting to mechanical stress4)Functional analysis of genes by gene manipulations and gene-disrupted mice5)Development of clinical application by experimental animal disease models(2)

Education

Purpose of Education
Organized signal networks in the body are crucial for the higher physiological functions and the tissue organization. To understand the regulation of signal events, we take on cell signaling course including the molecularmechanism of both the intra cellular and the inter cellular signal transduction. Especially, the course will
be focused on the molecular networks of signal transduction in osteoclasts, osteoblasts and osteocytes which is a
new integrated ﬁeld of osteonetwork (systemic network between bone and other systems). Besides, to promote
the practical and clinical understanding, the course will deal with the molecular mechanism of osteoporosis andinﬂammatory bone destructed diseases, such as periodontal disease and rheumatoid arthritis, in parallel withthe basic molecular biology.

(3)

Publications[Original Articles]
1. Takehito Ono, Kazuo Okamoto, Tomoki Nakashima, Takeshi Nitta, Shohei Hori, Yoichiro Iwakura, Hiroshi
Takayanagi. IL-17-producing γδ T cells enhance bone regeneration. Nat Commun. 2016.03; 7; 10928
2. Aritoshi Iida, Weirong Xing, Martine K F Docx, Tomoki Nakashima, Zheng Wang, Mamori Kimizuka,
Wim Van Hul, Dietz Rating, Jürgen Spranger, Hirohumi Ohashi, Noriko Miyake, Naomichi Matsumoto,Subburaman Mohan, Gen Nishimura, Geert Mortier, Shiro Ikegawa. Identiﬁcation of biallelic LRRK1
mutations in osteosclerotic metaphyseal dysplasia and evidence for locus heterogeneity. J. Med. Genet..
2016.04;
3. Asuka Terashima, Kazuo Okamoto, Tomoki Nakashima, Shizuo Akira, Koichi Ikuta, Hiroshi Takayanagi.
Sepsis-Induced Osteoblast Ablation Causes Immunodeﬁciency. Immunity. 2016.06; 44(6); 1434-1443― 113 ―

Bio-Matrix
Bio-Matrix[Misc]
1. Semaphorin and osteoporosis 2016.10; 26(10); 1419-1427― 114 ―

Bio-Matrix
Bio-Matrix

Periodontology

Professor Yuichi IZUMIAssociate Professor Hisashi WATANABE
Lecturer Akira AOKI
Research Associate
Yasuo TAKEUCHI, Tatuya AKIZUKI, Koji MIZUTANI, Sayaka KATAGIRI

Graduate Students
Yasuo ITO, Takahiko SHIBA, Shogo MAEKAWA, Ayano UEKUBO,
Wataru ONO, Hiroki SATO, Takeaki SUDO, Mizuki NAGATA, Kiichi MARUYAMA, Kosei YANO,
Chantida Pawauputanon Na Mahasarakham
Ammar Shujaa Addin,
Tooru TAKAGI, Anri OHTSU, Eri IKEDA (TAZAKI), Sayuri UDAGAWA,
Risako MIKAMI, Rina KOMAZAKI, Daisuke KIDO, Takashi ODE,
Sophannary Kong, Thatawee Khemwong, Nay Aung,
Shinta SUZUKI（April∼), Yuto OOSUGI, Kohei TAKEDA, Yosuke TUCHITANI, Shunsuke FUKUBA, Naho SUZUKI,
Kaori FUJIWARA, Naho SUZUKI,
Naoki SASAKI（April∼), Munehiro OKADA（April∼), Chihiro KANO（April∼), Yuji KATO（April∼), Rie KAWAMU
Hospital Staﬀ: 7, Research Student: 20, Registered dentist: 41(1)

OutlinePeriodontology is a branch of dental sciences which deals with the research, prevention and treatment of
periodontal diseases. Periodontal disease is a general disease name which occurred in the periodontal tissue:
gingiva, periodontium, cementum and alveolar bone. At present, it is indicated mainly an acute or chronic
inﬂammatory diseases. The mission of our department was to educate etiology of periodontal diseases, host
response, oral bacteria, periodontal medicine, regenerative therapy and so on profoundly, and to ﬁnd a solution
through discussion research outcomes as to periodontal destructive process and to develop a novel periodontal
treatment modalities.
(2)

Research

1) Periodontopathic bacteria and their pathogenicity
2) Inﬂammatory and immunological factors in periodontal diseases
3) Analyses of growth factors and bio materials in periodontal regeneration
4) Clinical applications of laser in periodontics
5) Inﬂuence of periodontal disease on general health(3)

Lectures & Courses

Periodontology is a branch of dental science which deals with supporting structures of teeth, diseases andconditions aﬀect them. Main objectives of periodontology in the graduate course is to provide students basic― 115 ―

Bio-Matrix
Bio-Matrix

knowledge of etiology of periodontal diseases, its treatment modality and prognosis, and also to study advanced
regenerative therapy.
(4)

Clinical Performances

Periodontal clinic provides diagnosis, treatment and prevention of periodontal disease. Periodontal surgery andregenerative therapy are also performed in the clinic.
(5)

Publications[Original Articles]
1. Thanakun S, Pornprasertsuk-Damrongsri S, Gokyu M, Kobayashi H, Izumi Y. Inverse Association of
Plasma IgG Antibody to Aggregatibacter actinomycetemcomitans and High C-Reactive Protein Levels in
Patients with Metabolic Syndrome and Periodontitis. PLoS ONE. 2016; 11(2); e0148638
2. Kobayashi N, Suzuki JI, Aoyama N, Sato H, Akimoto S, Wakayama K, Kumagai H, Ikeda Y, Akazawa H,
Komuro I, Izumi Y, Isobe M. Toll-like receptor 4 signaling has a critical role in Porphyromonas gingivalisaccelerated neointimal formation after arterial injury in mice. Hypertension Research. 2016; 39(10);717-722
3. Tsumanuma Y, Iwata T, Kinoshita A, Washio K, Yoshida T, Yamada A, Takagi R, Yamato M, Okano T,
Izumi Y. Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal
Cell Sheets in Canine Critical-Size Supra-Alveolar Periodontal Defect Model. BioResearch Open Access.
2016.01; 5(1); 22-36
4. Ikawa T, Akizuki T, Matsuura T, Hoshi S, Ammar S, Kinoshita A, Oda S, Izumi Y. Ridge Preservation After Tooth Extraction With Buccal Bone Plate Deﬁciency Using Tunnel Structured β-Tricalcium
Phosphate Blocks: A 2-Month Histologic Pilot Study in Beagle Dogs. J. Periodontol.. 2016.02; 87(2);175-183
5. Hoshi S, Akizuki T, Matsuura T, Ikawa T, Kinoshita A, Oda A, Tabata Y, Matsui M, Izumi Y. Ridge
augmentation using recombinant human ﬁbroblast growth factor-2 with biodegradable gelatin sponges
incorporating β-tricalcium phosphate: a preclinical study in dogs. J. Periodont. Res.. 2016.02; 51(1);77-85
6. Lin T, Kawamura R, Aoki A, Ichinose S, Mizutani K, Taniguchi Y, Eguro T, Saito N, Izumi Y. Energy
output reduction and surface alteration of quartz tips following Er:YAG laser contact irradiation on softand hard tissues in vitro. Dental Materials Journal. 2016.02; 35(1); 51-62
7. Khamaisi M, Katagiri S, Keenan H, Park K, Maeda Y, Li Q, Qi W, Thomou T, Eschuk D, Tellechea
A, Veves A, Huang C, Orgill DP, Wagers A, King GL. PKC δ inhibition normalizes the wound-healing
capacity of diabetic human ﬁbroblasts. J. Clin. Invest.. 2016.03; 126(3); 837-853
8. Kitamura M, Akamatsu M, Kawanami M, Furuichi Y, Fujii T, Mori M, Kunimatsu K, Shimauchi H, Ogata
Y, Yamamoto M, Nakagawa T, Sato S, Ito K, Ogasawara T, Izumi Y, Gomi K, Yamazaki K, Yoshie H,
Fukuda M, Noguchi T, Takashiba S, Kurihara H, Nagata T, Hamachi T, Maeda K, Yokota M, Sakagami
R, Hara Y, Noguchi K, Furuuchi T, Sasano T, Imai E, Ohmae M, Koizumi H, Watanuki M, Murakami
S. Randomized Placebo-Controlled and Controlled Non-Inferiority Phase III Trials Comparing Trafermin, a Recombinant Human Fibroblast Growth Factor 2, and Enamel Matrix Derivative in Periodontal
Regeneration in Intrabony Defects. J. Bone Miner. Res.. 2016.04; 31(4); 806-814
9. Shiheido Y, Maejima Y, Suzuki J, Aoyama N, Kaneko M, Watanabe R, Sakamaki Y, Wakayama K,
Ikeda Y, Akazawa H, Ichinose S, Komuro I, Izumi Y, Isobe M. Porphyromonas gingivalis, a periodontal
pathogen, enhances myocardial vulnerability, thereby promoting post-infarct cardiac rupture. J. Mol.
Cell. Cardiol.. 2016.04;
10. Miyazaki H, Ito Y, Ohshiro T, Kato J, Romeo U, Nomura T, Makiguchi T, Ogawa M, Watanabe H, Yokoo
S . Laser treatments of oral vascular lesions - Approach to the oral vascular lesions with the intalesionallaser photocoagulation technique - Journal of Japanese Society for Laser Surgery and Medicine. 2016.04;
37(1); 58-65― 116 ―

Bio-Matrix
Bio-Matrix

11. Thanakun S, Izumi Y. Eﬀect of Periodontitis on Adiponectin, C-Reactive Protein, and Immunoglobulin
G Against Porphyromonas gingivalis in Thai People With Overweight or Obese Status. J. Periodontol..
2016.05; 87(5); 566-576
12. Park K, Mima A, Li Q, Rask-Madsen C, He P, Mizutani K, Katagiri S, Maeda Y, Wu IH, Khamaisi M,
Preil SR, Maddaloni E, Sørensen D, Rasmussen LM, Huang PL, King GL. Insulin decreases atherosclerosis
by inducing endothelin receptor B expression. JCI Insight. 2016.05; 1(6); e86574
13. Tooi M, Komaki M, Morioka C, Honda I, Iwasaki K, Yokoyama N, Ayame H, Izumi Y, Morita I. Placenta
Mesenchymal Stem Cell Derived Exosomes Confer Plasticity on Fibroblasts. J. Cell. Biochem.. 2016.07;
117(7); 1658-1670
14. Suzuki J, Imai Y, Aoki M, Fujita D, Takeda N, Aoyama N, Wakayama K, Ikeda Y, Kumagai H, Akazawa
H, Izumi Y, Isobe M, Komuro I, Hirata Y. Periodontitis May Deteriorate Sinus of Valsalva Dilatation in
Marfan Syndrome Patients. Int Heart J. 2016.07; 57(4); 456-460
15. Noda M, Aoki A, Mizutani K, Lin T, Komaki M, Shibata S, Izumi Y. High-frequency pulsed low-level
diode laser therapy accelerates wound healing of tooth extraction socket: An in vivo study. Lasers inSurgery and Medicine. 2016.07; 48(10); 955-964
16. Taniguchi Y, Aoki A, Sakai K, Mizutani K, Meinzer W, Izumi Y. A Novel Surgical Procedure for Er:YAG
Laser-Assisted Periodontal Regenerative Therapy: Case Series. Int J Periodontics Restorative Dent.
2016.07; 36(4); 507-515
17. Taniguchi JB, Kondo K, Fujita K, Chen X, Homma H, Sudo T, Mao Y, Watase K, Tanaka T,Tagawa
K, Tamura T, Muramatsu SI, Okazawa H. RpA1 ameliorates symptoms of mutant Ataxin-1 knock-inmice and enhances DNA damage repair. Hum Mol Genet. 2016.;  
18. Shiba T, Watanabe T, Kachi H, Koyanagi T, Maruyama N, Murase K, Takeuchi Y, Maruyama F, Izumi
Y, Nakagawa I. Distinct interacting core taxa in co-occurrence networks enable discrimination of polymicrobial oral diseases with similar symptoms. Sci Rep. 2016.08; 6; 30997
19. Katagiri S, Park K, Maeda Y, Rao TN, Khamaisi M, Li Q, Yokomizo H, Mima A, Lancerotto L, Wagers
A, Orgill DP, King GL. Overexpressing IRS1 in Endothelial Cells Enhances Angioblast Diﬀerentiationand Wound Healing in Diabetes and Insulin Resistance. Diabetes. 2016.09; 65(9); 2760-2771
20. Akazawa ,, Iwasaki ,, Nagata ., Yokoyama /, Ayame ), Yamaki ,, Tanaka :, Honda *, Morioka $,
Kimura 5, Komaki ., Kishida ", Izumi :, Morita*. Double-layered cell transfer technology for boneregeneration. Sci Rep.2016.09; 6; 33286
21. Lee BS, Lee CC, Lin HP, Shih WA, Hsieh WL, Lai CH, Takeuchi Y, Chen YW. A functional chitosan
membrane with grafted epigallocatechin-3-gallate and lovastatin enhances periodontal tissue regenerationin dogs. Carbohydr Polym. 2016.10; 151; 790-802
22. Onizuka S, Iwata T, Park S, Nakai K, Yamato M, Okano T, Izumi Y. ZBTB16 as a Downstream Target
Gene of Osterix Regulates Osteoblastogenesis of Human Multipotent Mesenchymal Stromal Cells. J. Cell.
Biochem.. 2016.10; 117(10); 2423-2434
23. Ogata Y, Nakayama Y, Tatsumi J, Kubota T, Sato S, Nishida T, Takeuchi Y, Onitsuka T, Sakagami R,
Nozaki T, Murakami S, Matsubara N, Tanaka M, Yoshino T, Ota J, Nakagawa T, Ishihara Y, Ito T, Saito
A, Yamaki K, Matsuzaki E, Hidaka T, Sasaki D, Yaegashi T, Yasuda T, Shibutani T, Noguchi K, Araki
H, Ikumi N, Aoyama Y, Kogai H, Nemoto K, Deguchi S, Takiguchi T, Yamamoto M, Inokuchi K, Ito T,
Kado T, Furuichi Y, Kanazashi M, Gomi K, Takagi Y, Kubokawa K, Yoshinari N, Hasegawa Y, Hirose
T, Sase T, Arita H, Kodama T, Shin K, Izumi Y, Yoshie H. Prevalence and risk factors for peri-implantdiseases in Japanese adult dental patients. J Oral Sci. 2016.10; e-pub;
24. Nagata M, Nagata M, Kanie T, Shima K. Clinical and Histologic Aspects of Cervical Cemental Tear as a
Risk for Periodontal Diseases. Clinical Advances in Periodontics. 2016.11; 6(4); 167-174
25. Ito H, Numabe Y, Hashimoto S, Sekino S, Murakshi E, Ishiguro H, Sasaki D, Yaegashi T, Takai H,
Mezawa M, Ogata Y, Watanabe H, Hagiwara S, Izumi Y, Hiroshima Y, Kido J, Nagata T, Kunimatsu
K. Correlation between gingival crevicular ﬂuid hemoglobin content and periodontal clinical parameters
J Periodontol. 2016.11; 87(11); 1314-1319― 117 ―

Bio-Matrix
Bio-Matrix

26. Lin T, Aoki A, Saito N, Yumoto M, Nakajima S, Nagasaka K, Ichinose S, Mizutani K, Wada S, Izumi
Y. Dental hard tissue ablation using mid-infrared tunable nanosecond pulsed Cr:CdSe laser. Lasers Surg
Med. 2016.12; 48(10); 965-977
27. Morozumi T, Nakagawa T, Nomura Y, Sugaya T, Kawanami M, Suzuki F, Takahashi K, Abe Y, Sato S,
Makino-Oi A, Saito A, Takano S, Minabe M, Nakayama Y, Ogata Y, Kobayashi H, Izumi Y, Sugano N,
Ito K, Sekino S, Numabe Y, Fukaya C, Yoshinari N, Fukuda M, Noguchi T, Kono T, Umeda M, Fujise
O, Nishimura F, Yoshimura A, Hara Y, Nakamura T, Noguchi K, Kakuta E, Hanada N, Takashiba S,
Yoshie H. Salivary pathogen and serum antibody to assess the progression of chronic periodontitis: a24-mo prospective multicenter cohort study. J. Periodont. Res.. 2016.12; 51(6); 768-778
28. Uekubo A, Hiratsuka K, Aoki A, Takeuchi Y, Abiko Y, Izumi Y. Eﬀect of antimicrobial photodynamic
therapy using rose bengal and blue light-emitting diode on Porphyromonas gingivalis in vitro: Inﬂuenceof oxygen during treatment Laser Therapy. 2016.12; 25(4); 299-308[Books etc]
1. Izumi Y. NHK Today’s Health June issue. NHK Publishing, 2016.05
2. Izumi Y. The latest countermeasures of "muscle" "bone" "teeth" "dementias". SHUFU TO SEIKATSUSHA CO., 2016.09 (ISBN : 978-4391638776)
3. Izumi Y, Nikaidou M. Correspondence to vertical bone defects. Igaku Joho-sha, 2016.10
4. Izumi Y, Oda S,Takeuchi Y, Mizutani K. Incident and Recovery of periodontal surgery Q&A96. Quintessence, 2016.11
5. Watanabe H. Ultra-Illustrated Periodontal Disease. Hoken, 2016.12 (ISBN : 978-4-86513-281-6)
6. Izumi Y, Akizuki T. Periodontal treatment 1 - Periodontal tissue examination, initial treatment -. Morita,
2016.12[Misc]
1. Taniguchi Y, Aoki A, Mizutani K, Izumi Y. Periodontal therapy applying features of Er:YAG laser JSLSM.
2016.03; 37(1); 43-51
2. Aoki A. Introdacution of laser institution JSLSM. 2016.03; 37(1); 124-126
3. Mizutani K, Aoki A, Coluzzi D, Yukna R, Wang C, Pavlic V, Izumi Y. Lasers in minimally invasive
periodontal and peri-implant therapy. Periodontol. 2000. 2016.06; 71(1); 185-212
4. Aoki A, Takeuchi Y, Hiratsuka K, Izumi Y. Antibacterial photodynamic therapy inperiodontal therapy- Current status of aPDT in clinical and Latest research on LED applications Journal of Clinical andExperimental Medicine. 2016.09; 258(11); 1058-1062
5. Takeuchi Y. General risk factors for peri-implant disease Journal of the Japanese Society of Oral Implantology. 2016.12; 29(4); 219-225[Conference Activities & Talks]
1. Aoki A. New periodontal therapy using lasers - Periodontal Phototherapy. The 36th Laser Society Annual
Meeting 2016.01.10 Nagoya
2. Izumi Y. The relation between general health condition and periodontal disease as oral infectious disease.
The 19th Annual Scientiﬁc Meeting of the Japan Society of Metabolism and Clinical Nutrition 2016.01.10
Yokohama
3. Aoki A. Periodontal treatment with Er:YAG laser seen in the light of basic and clinical evidence . Er:
YAG Laser Users Conference 2016.01.17 Omiya
4. Aoki A. Periodontal Er:YAG Laser Operation. Tokyo Medical and Dental University Dental Alumni
association CDE Practice course 2016.02.21 Tokyo― 118 ―

Bio-Matrix
Bio-Matrix

5. Shiba T, Watanabe T, Kachi H, Maruyama F, Takeuchi Y, Koyanagi T, Maruyama N, Murase K, Izumi
Y, Nakagawa I. Dissimilarities of functional keystone species in co-occurrence network enable discrimination of intractable polymicrobial infectious diseases. SMBE Satellite Workshop on Genome Evolution in
Pathogen Transmission and Disease 2016.02.24 Nagano
6. Watanabe H. Basis of periodontol diseases and its ﬂow of periodontal therapy. GC dental college 2016.02.29
Tokyo
7. Akizuki T. Role of the high bioavailable curcumin of the treatment and prevention of the life-style diseses.
Symposium 21. The 89th Annual Meeting of the Japanese Pharmacological Society 2016.03.10 Yokohama
8. Ikeda E, Takeuchi Y, Suda W, Ikeda Y, Nakasato A, Hattori M, Izumi Y. Pyrosequencing analysis of
subgingival microbiota in chronic periodontitis. 45th Annual Meeting and Exhibition of the American
Association for Dental Research 2016.03.18 Los angeles, USA
9. Kido D, Maruyama K, Izumi Y. A case report of comprehensive treatment for a patient with generalized
severe chronic periodontitis based on a bacteriological examination. The 30th anniversary KYUSYUIMPLANT RESEARCH GROUP (KIRG) workshop 2016.03.26 Fukuoka
10. Koyanagi T, Kobayashi H, Takeuchi S, Izumi Y. Analysis of peri-implant bone defect conﬁguration using
cone beam CT. 2016.03.26
11. Otsu A, Suda T, Takeuchi Y, Izumi Y. Clinical eﬀect of sodium hypochloriet diution water applied indental supply duct line. 17th Annual meeting of the Japan Society for Oral Functional Water 2016.03.27
Osaka
12. Sudo T, Okada Y, Kobayashi H, Gokyu M, Tanaka T, Izumi Y. Whole-exome sequencing reveals a novel
gene as a cause of Aggressive periodontitis in Japanese consanguineous families. The 13th International
Congress of Human Genetics 2016.04.04 Kyoto
13. Sugawara Y, Kashimada A, Moriyama K, Baba S, Imai K, Nishikomori R, Motegi M, Takeuchi Y, Morio
T. Aicardi-Goutieres and Singleton-Merten overlapping phenotype due to IFIH1 mutation.. The 13th
International Congress of Human Genetics 2016.04.06 Kyoto, Japan
14. Taniguchi Y. Best clinical case clinical case description. Geistlich Biomaterials 2016.04.12 on line
15. Aoki A. Er:YAG Laser in Periodontics.
2016.04.14 Orlando,USA

The 23rd Academy of Laser Dentistry Annual Conference

16. Watanabe H. Novel dental therapy developed by laser. 6th annual meeting of Japan Association of Dental
traumatology 2016.04.17 Tokyo
17. Ikawa T, Akizuki T, Matsuura T, Hoshi S, Ono W, Maruyama K, Shujaa Addin A, Izumi Y. Histologicalevaluation of the tunnel β-tricalcium phosphate blocks for ridge preservation after tooth extraction withbuccal bone deﬁciency in beagle dogs. Osteology International Symposium 2016.04.21 Principauté deMonaco18. Mikami R, Aoki A, Mizutani K, Tamura Y, Aoki K, Izumi Y. Low-level laser irradiation using a highfrequency ultra-short pulsed blue laser enhances osteoblasts proliferation and diﬀerentiation. The 59th
Spring Meeting of The Japanese Society of Periodontology 2016.05.20 Kagoshima
19. Aoki A. Eﬀects of lights on periodontal pathogens and basic studies of aPDT - Applications of LED.
Symposium Ⅰ: Is antimicrobial photodynamic therapy eﬀective in the periodontal therapy ?. The 59th
Spring Meeting of the Japanese Society of Periodontology 2016.05.20 Kagoshsima
20. Yashima A, Morozumi T, Ujiie Y, Yoshie H, Hokari T, Izumi Y, Mizutani K, Takamatsu H, Minaba M,
Akizuki T, Miyauchi S, Yoshino T, Tanaka M, Tanaka Y, Gomi K. Eﬀectiveness of Azythromycin on
one-stage full-mouth SRP. -Biological response and clinical outcome-. The 59th Spring Meeting of The
Japanese Society of Periodontology. 2016.05.20 Kagoshima
21. Morozumi T, Yashima A, Gomi K, Ujiie Y, Izumi Y, Mizutani K, Takamatsu H, Minabe M, Akizuki
T, Miyaushi S, Yoshino T, Tanaka M, Tanaka Y, Hokari T, Yoshie H. Biological response and Clincicaloutcome after application of one-stage full-mouth SRP. The 59th Spring Meeting of The Japanese Societyof Periodontology. 2016.05.20 Kagoshima― 119 ―

Bio-Matrix
Bio-Matrix

22. Katagiri S, Shigaki-Katagiri A, Takeuchi Y. A case report of oral rehabilitation for severe chronic periodontitis with occlusal trauma. The 59th Spring Meeting of The Japanese Society of Periodontology
2016.05.21 Kagoshima
23. Shiba T, Watanabe T, Koyanagi T, Maruyama N, Takeuchi Y, Maruyama F, Nakagawa I, Izumi Y.
Homeostasis maintenance mechanism inferred from polymicrobial oral diseases with similar symptoms.
2016.05.21 Kagoshima
24. Komaki M, Aoki A, Iwasaki K, Izumi Y. Coverage of class III gingival recession localized at anterior
mandibular teeth using connective tissue graft. The 59th Spring Meeting of The Japanese Society of
Periodontology 2016.05.21
25. Shujaa Addin A, Akizuki T, Hoshi S, Matsuura T, Ikawa T, Maruyama K, Ono W, Fukuba S, Izumi Y.
Eﬀect of rhFGF-2 Combined with Gelatin/β-TCP Sponge on Root Coverage: a Pilot Study in Beagle
Dogs. The 59th Spring Meeting of The Japanese Society of Periodontology 2016.05.22 Kagoshima
26. Aoki A. Current status of Er:YAG Laser in periodontics and peri-implant therapy - From basic studies
to clinical applications: Part I and Part II. 10th Congress of the Asia Paciﬁc Laser Institute 2016.05.29
Taipei, Taiwan
27. Takeuchi S, Koyanagi T, Ogawa M, Ikawa T, Suda T, Takeuchi Y, Izumi Y. The application of corn beam
computed topography data for the diagnosis of furcation defects. The 144th Meeting of the JapaneseSociety of Conservative Dentistry 2016.06.06 Utsunomiya
28. Tooi M, Komaki M, Iwasaki K, Yokoyama N, Nagata M, Izumi Y, Morita I. Placenta mesenchymal stem
cell derived exosomes alter diﬀerentiation competence in ﬁbroblasts. The 144th Meeting of the JapaneseSociety of Conservative Dentistry 2016.06.09 Utsunomiya
29. Akazawa K, Iwasaki K, Komaki M, Yokoyama N, Izumi Y, Morita I. Construction of double-layered celltransferred-amnion using osteoblasts and periodontal ligament stem cells by cell transfer technology. The
37th Spring Meeting of The Japanese Society of Inﬂammation and Regeneration 2016.06.17 Kyoto
30. Watanabe H. Novel periodontal plastic surgery using Er:YAG laser. 94th General Session & Exhibitionof the International Association for Dental Research (IADR) 2016.06.22 Seoul, Republic of Korea
31. Shujaa Addin A, Akizuki T, Hoshi S, Matsuura T, Ikawa T, Maruyama K, Ono W, Fukuba S, Izumi Y.
Eﬀect of rhFGF-2/Gelatin-β-TCP on Root Coverage in Beagle Dogs. 94th General Session & Exhibitionof the International association for Dental Research Meeting 2016.06.23 Seoul, Republic of Korea.
32. Shinkai K, Oda S, Yamaguch K, Yamada M, Yoshikawa K, Mitani A, Watanabe H. A proposal of guideline
for lecture of laser dentistry. 35th annual meeting of Japanese Dental Education Association 2016.07.02
Osaka
33. Watanabe C, Wada J, Mizutani K, Watanabe H, Wakabayashi N. The eﬀect of removable partial denture
placement on periodontal structures of abutment teeth in patients of periodontitis: A digital subtraction
analysis. The 125th Scientiﬁc Meeting of Japan Prosthodontic Society 2016.07.10 Kanazawa
34. Izumi Y. The clinical epidemiologic survey on periodontal conditions of patients with cardiovasculardiseases. The 48th Annual Scientiﬁc Meeting of the Japan Atherosclerosis Society 2016.07.14 Tokyo
35. Aoki A. Soft tissue application. Basic Laser Certiﬁcation Course, 15th Congress of the World Federation
for Laser Dentistry 2016.07.16 Nagoya
36. Lin Tai-Chen, Aoki A, Kawamura T, Takagi T, Uekubo A, Mikami R, Mizutani K, Izumi Y. Soft andhard tissue ablation using a Q-switched 2.81 μ m Er:ZBLAN ﬁber laser. The 28th Annual Meeting ofJapanese Society for Laser Dentistry 2016.07.16 Nagoya
37. Taniguchi Y. A novel surgical procedure of Er:YAG laser-assisted bone regenerative therapy. 15th Congressof the World Federation for Laser Dentistry 2016.07.16 Nagoya
38. Watanabe H. Novel periodontics in laser dentistry. 15th Congress of World Federation for Laser Dentistry2016.07.17 Nagoya― 120 ―

Bio-Matrix
Bio-Matrix

39. Watanabe H, Belal MH. Comparative study on morphologic changes and cell attachment of periodontitisaﬀected root surfaces followingconditioning with CO2 and Er:YAG laser irradiations. 15th Congress ofthe World Federation for Laser Dentistry 2016.07.17 Nagoya
40. Aoki A, Mizutani K, Taniguchi Y, Komaki M, Oda S, Watanabe H, Izumi Y. Er:YAG Laser-assisted
comprehensive periodontal pocket therapy (Er-LCPT): case series. 15th Congress of the World Federation
for Laser Dentistry 2016.07.17 Nagoya
41. Eguro T, Takanori Suzuki T, Aoki A, Mizoguchi T, Izumi Y. Soft tissue management with the Er:YAG
laser in implant therapy. 15th Congress of the World Federation for Laser Dentistry 2016.07.17 Nagoya
42. Miyazaki H, Romeo U, Wada Y, Vecchio AD, Kato J, Tanaka K, Noguchi T, Maruoka Y, Watanabe H,
Harada H, Asamura S. Treatment strategies for oral vascular anomalies using intralesional laser photocoagulation. 15th Congress of World Federation for Laser Dentistry 2016.07.18 Nagoya
43. Tsukui A, Watanabe H. Clinical applications of Er:YAG laser in peri-implantitis therapy - Case report.
15th Congress of World Federation for Laser Dentistry 2016.07.18 Nagoya
44. Yano K, Washio K, Tsumanuma Y, Iwata T, Yamato M, Ohta K, Okano T, Izumi Y . Phenotypic analysisof bone and cartilage in Tsukushi-deﬁcient mice. The 34th Annual Meeting of the Japanese Society forBone and Mineral Research 2016.07.21 Osaka
45. Komaki M, Tooi M, Iwasaki K, Morita I . Mesenchymal stem cell derived exosomes alter diﬀerentiationcompetence in ﬁbroblasts. The 34th Anuual Meeting of the Japanese Society for Bone and Mineral
Research 2016.07.22 Osaka
46. Taniguchi Y. The application of Er:YAG laser-assisted bone reganerative therapy for periodontics andimplant treatment: case report and bibliographical consideration . Clinical research institute for Er:YAG
laser treatment, 19th meeting 2016.08.07 Tokyo
47. Watanabe T, Shiba T, Kachi H, Takeuchi Y, Maruyama F, Izumi Y, Nakagawa I. Metatranscriptomic
analyses of microbiota in peri-implantitis and periodontitis lesions. The 58th Annual Meeting of Japanese
Association for Oral Biology 2016.08.25 Sapporo
48. Iwata T, Yamato M, Washio K, Tsumanuma Y, Yamada A, Onizuka S, Izumi Y, Ando T, Okano T,
Ishikawa I. Periodontal Regeneration with Autologous Periodontal Ligament-derived Cell Sheets in humans.. TERMIS-AP2016 2016.09.04 Taipei, Taiwan
49. Benhamou V, Sarmiento HL, Aoki A. Er:YAG laser: Hands-on course, Er: YAG Lasers - Daily Applications in Periodontics: Training Course. Morita Er:YAG laser user meeting 2016.09.09 San Diego,USA
50. Aoki A. Er:YAG Laser in Periodontics and Implant Therapy - From Basic Studies to Clinical Applications.
Er: YAG Lasers - Daily Applications in Periodontics: Training Course. Morita Er:YAG laser user meeting2016.09.09 San Diego, USA
51. Aoki A. Current status of Er:YAG laser therapy in management of peri-implant disease. 5th Pan Paciﬁc
Session Implant Therapy For the Periodontally Compromised Patient. The 102nd Annuaml Meeting ofAmerican Academy of Periodontology 2016.09.09 San Diego, USA
52. Mikami R, Aoki A, Mizutani K, Tamura Y, Aoki K, Izumi Y. Low-level blue laser irradiation enhancesosteoblast proliferation and diﬀerentiation. The 102nd Annual Meeting of the American Academy of
Periodontology 2016.09.10 San Diego, USA
53. Maekawa S, Katagiri S, Takeuchi Y, Komazaki R, Ohtsu A, Udagawa S, Izumi Y. Bone metabolic microarray analysis of ligature-induced periodontitis in streptozotocin- induced diabetic mice. The 102nd
Annual Meeting of American Academy of Periodontology 2016.09.10 San Diego, USA
54. Ono W, Ogiso M, Maruyama K, Mineno S, Izumi Y. Histological analysis of implantation to augmentedbone using Casing Method". The 102nd Annual Meeting of the American Academy of Periodontology
2016.09.10 San Diego, USA
55. Maruyama K, Ogiso M, Ono W, Mineno S, Izumi Y. The histological analysis of great-sized ridge augmentation applying the Casing Method . The 102nd Annual Meeting of the American Academy of
Periodontology 2016.09.10 San Diego, USA― 121 ―

Bio-Matrix
Bio-Matrix

56. Shiba T, Watanabe T, Koyanagi T, Maruyama N, Takeuchi Y, Maruyama F, Izumi Y, Nakagawa I.
Functional keystone species enable discrimination of peri-implantitis and periodontitis. The 102nd Annual
Meeting of the American Academy of Periodontology 2016.09.10 San Diego, USA
57. Shujaa Addin A, Hoshi S, Matsuura T, Ikawa T, Maruyama K, Ono W, Fukuba S, Izumi Y. rhFGF2/gelatin-β TCP in the treatment of gingival recessions in dogs. The 102nd meeting of American Academyof Periodontology 2016.09.12 San Deigo, USA
58. Watanabe H. Novel periodontal therapy using Er:YAG laser- Basis and clinical outcome. The 102nd
Annual Meeting of the American Academy of Periodontology 2016.09.13 San Diego, USA
59. Takagi T, Aoki A, Lin T, Taniguchi Y,Tachikawa N, Shinoki T, IzumiI Y. The eﬀectiveness of Erbium
family laser on dental implant surface -Investigation of appropriate parameter for irradiation and removability of artiﬁcial calciﬁed deposits on dental implant-. The 46th Annual Meeting of the Japanese Societyof Oral Implantology 2016.09.17 Nagoya
60. Taniguchi Y, Koyanagi T, Lin T, Izumi Y. Simultaneous ﬁxture placement with various bone augmentation
techniques using new designed ﬁxtures: a case report. Europian association for osseointegration 25th
annual scientiﬁc meeting 2016.09.30 Paris, France
61. Masahiro Noda, Akira Aoki, Koji Mizutani, Taichen Lin, Motohiro Komaki, Shunichi Shibata, Yuichi
Izumi. Eﬀect of low level laser irradiation on early stage of wound healing of tooth extraction socket. The
23rd General Meeting of the Japanese Association for Dental Science 2016.10 Fukuoka
62. Tsubokawa M, Aoki A, Kakizaki S, Lin T, Mizutani K, Oda S, Sumi Y, Izumi Y. Optical measurementof periodontal pocket depth using optical coherence tomography (OCT) . The 59th Autumn Meeting of
The Japanese Society of Periodontology 2016.10.06 Niigata
63. Maruyama K, Ogiso M, Ono W, Mineno S, Izumi Y. The histological analysis of great-sized ridge augmentation applying the "Casing Method". The 59th Autumn Meeting of The Japanese Society of Periodontology 2016.10.07 Niigata
64. Kakizaki S, Mizutani K, Osugi Y, Katagiri S, Aoki A, Izumi Y. The questionnaire survey about explanationof intra-oral photography using iPad in dental student clinic. The 59th Autumn Meeting of The JapaneseSociety of Periodontology 2016.10.07 Niigata
65. Nagata M, Iwasaki K, Akazawa K, Komaki K, Yokoyama N, Izumi Y, Morita I. Conditioned medium
from periodontal ligament stem cells enhances periodontal regeneration. The 59th Autumn Meeting of
The Japanese Society of Periodontology 2016.10.07 Niigata
66. Aoki A. Er:YAG laser-assisted comprehensive periodontal pocket therapy. the 23rd General Meeting of
the Japanese Association for Dental Science 2016.10.21 Fukuoka
67. Watanabe H, Shinoki T(Moderater), Yoshimine Y, Fujitani M, Aoki A. Symposium: Inovation of conservative dentistry by laser. The 23rd General Meeting of The Japanese Association for Dental Science2016.10.22 Fukuoka
68. Aoki A, Mizutani K, Taniguchi Y, Watanabe H, Izumi Y. Application of Er:YAG laser in periodontal
pocket therapy and ﬂap surgery. The 23rd General Meeting of the Japanese Association for Dental
Science 2016.10.23 Fukuoka
69. Ogawa M, Koyanagi T, Takeuchi T, Katagiri S, Ikawa T, Takeuchi S, Sekiuchi T, Arai Y, Kazama R,
Wakabayashi N, Izumi Y. Evaluation of palatal mucosal thickness measurements using CBCT. 145th
Meeting of the Japanese Society of Conservative Dentistry 2016.10.28 Matsumoto
70. Aoki A. Periodontal Disease - Pathogenesis, Diagnosis and Management. 2016 Tokyo Medical and Dental University - Taipei Medical University.Joint Symposium on Immunity, Inﬂammation, and Infection
2016.11.12 Taipei, Taiwan
71. Aoki A. Eﬀects of ultraviolet LEDs on periodontal pathogenic bacteria. Heisi 28 ﬁscal year Development
cost of longevity medical research 26-6 "Research on the development of new diagnostic and treatment
devices using infrared lights and lasers" Group meeting 2016.11.17 Obu
72. Aoki A. Application of Er:YAG laser in periodontal therapy. The 18 th Advanced Laser Application
Technology Seminar 2016.11.17 Yokohama― 122 ―

Bio-Matrix
Bio-Matrix

73. Ikeda E, Grazieli M, Ikeda Y, Wang y, Fine N, Sheikh Z, Casati M, Ganss B, Glogauer M, Tenenbaum
H. Antioxidants/Aryl hydrocarbon-Receptor Antagonist Resveratorol and Curcumin Both Prevent andReverse Periodontitis-Mediated Bone Loss in Rodents. 4th international conference of resveratrol andhealth 2016.11.18 Taipei, Taiwan
74. Watanabe H. Laser dentistry - its basis and clinical application . 4th educational seminar of Japanese
Association for Laser Dentistry 2016.11.24 Sapporo
75. Hatase M, Watanabe H, Ejiri K, Tsumanuma Y, Izumi Y. Role of Calprotectin in Periodontal Diagnosis.
The 81st Annual Meeting of the Stomatological Society 2016.11.26 Tokyo
76. Ode T, Podyma-Inoue KA, Terasawa K, Watabe T, Izumi Y, Yokoyama M. PDMP, a ceramide analogue,
acts as an inhibitor of mTORC1 by inducing its translocation from lysosome to endoplasmic reticulum.
The 81th Annual Meeting of the Stomatological Society 2016.11.26 Tokyo
77. Aoki A. Periodontal Er:YAG Laser Operation. Dental Alumni Association Tokyo Medical and Dental
University CDE Practical training course 2016.12.04 Tokyo
78. Takahashi H, Katagiri S, Eguchi Y. Periodontal Pathogens aﬀect on NAFLD/NASH. The 41st meetingof Eastern Branch of the Japan Society of Hepatology 2016.12.09 Tokyo[Awards & Honors]
1. The 37th Spring Meeting of The Japanese Society of Inﬂammation and Regeneration. Excellence Award(Akazawa K), The Japanese Society of Inﬂammation and Regeneration, 2016
2. 2016 Research encouragement award for postgraduate student (Sudo T), 2016.03
3. 2016 Morita Scholarship Foundation (Sudo T), Morita Scholarship Foundation, 2016.07
4. Young Researcher’s Award, Japanese Society of Laser Dentistry (Sawabe M), The 28th Annual Meetingof Japanese Society for Laser Dentistry, 2016.07
5. 2016 Iwadare Scholarship Foundation (Sudo T), Iwadare Scholarship Foundation, 2016.08
6. Sunstar Young Investigator Award (Nagata M), The Japanese Society of Periodontology, 2016.10[Others]
1. Platina Life TOKYO MX TV (Aoki A), 2016.02
Periodontology and Alzheimer disease
2. Platina Life TOKYO MX TV (Aoki A), 2016.02
Periodontology and Premature labor― 123 ―

Public HealthPublic Health

Global Health Promotion

Professor: Takeo Fujiwara, MD, MPH, PhD (Apr-)
Assistant Professor: Keiko Nakamura, MD, PhD (-Mar)
Junior Associate Professor: Masashi Kizuki, MD, MPH, PhD; Kaoruko Seino, PhD (-Mar)
Assistant Professor: Ayako Morita, PhD
Research Fellow of Japan Society for the Promotion of Science: Yukako Tani, PhD; Satomi Doi, PhD（Apr-Sep)
Project Researcher: Aya Isumi, PhD; Satomi Doi, PhD (Oct-)(1)

Outline

The purpose of this course is to develop the knowledge and skills of the participants to prevent diseases. Participants will: understand broad risk factors from indiviual factors (e.g., genetic factor) and environmental factors,
especially social detrerminants, their inter actions; make causal inference applying a life-course perspective on
diseease onset (e.g., long-term eﬀect of fetus or childhood exposure); perform advanced statstics; acquire attitudes toward social contribution through writeing and publishing scientiﬁc papers in international journals.
The ﬁnal goal is that the participants are able to plan and implement health policy or preogran to prevent
diseases in a real life setting.
(2)

Research

The main focus of the department is as follows:
1. Social epidemiology (impact of social inequality, social capital, social network, and social support on health)
2. Life-course epidemiology (impact of child poverty and adverse childhood experiences on health) and international comparison study
3. Prevention on child abuse and neglect
4. Disaster and child and their family’s mental health
5. Nutrition during pregnancy or early childhood and health(3)

Education

The faculty of department teach Public Health and Social Medicine for grade 3 medical students, Public Health
Practice for grade 4 medical students, and graduate students seminars.
(4)

Lectures & Courses

The purpose of this course is to develop the knowledge and skills of the participants to prevent diseases. Participants will: understand broad risk factors from indiviual factors (e.g., genetic factor) and environmental factors,
especially social detrerminants, their inter actions; make causal inference applying a life-course perspective on
diseease onset (e.g., long-term eﬀect of fetus or childhood exposure); perform advanced statstics; acquire attitudes toward social contribution through writeing and publishing scientiﬁc papers in international journals.
The ﬁnal goal is that the participants are able to plan and implement health policy or preogran to prevent
diseases in a real life setting.
The participants will be able to: 1. explain the risk of disease.
2. verbalize own research question and develop a hypothesis to test it.

― 124 ―

Public HealthPublic Health

3. develop research ﬁeld or access secondary data to test the hypothesis.
4. explain an epidemiologic study design.
5. calculate a sample size.
6. analyse basic model (multivariate analysis, logistic analysis, etc) and conduct adnvaced analysis (multilevel
analysis, propensity score moathcing, multiple imputation, etc)
7. justify the research question logically, in scientiﬁc writing in English.
8. develop an intervention (policy or program) and design a study protocol to assess its eﬀectiveness.
(5)

Publications[Original Articles]
1. Sachiko Baba, Hiroyasu Iso, Takeo Fujiwara. Area-Level and Individual-Level Factors for Teenage Motherhood: A Multilevel Analysis in Japan. PLoS ONE. 2016; 11(11); e0166345
2. Akiko Mizuta, Takeo Fujiwara, Toshiyuki Ojima. Association between economic status and body massindex among adolescents: a community-based cross-sectional study in Japan. BMC Obes. 2016; 3; 47
3. Yosuke Inoue, Andrew Stickley, Aki Yazawa, Kokoro Shirai, Airi Amemiya, Naoki Kondo, Katsunori
Kondo, Toshiyuki Ojima, Masamichi Hanazato, Norimichi Suzuki, Takeo Fujiwara. Neighborhood Characteristics and Cardiovascular Risk among Older People in Japan: Findings from the JAGES Project.
PLoS ONE. 2016; 11(10); e0164525
4. Naho Morisaki, Takeo Fujiwara, Reiko Horikawa. The Impact of Parental Personality on Birth Outcomes:
A Prospective Cohort Study. PLoS ONE. 2016; 11(6); e0157080
5. Seung Chik Jwa, Kohei Ogawa, Minatsu Kobayashi, Naho Morisaki, Haruhiko Sago, Takeo Fujiwara.
Validation of a food-frequency questionnaire for assessing vitamin intake of Japanese women in early andlate pregnancy with and without nausea and vomiting. J Nutr Sci. 2016; 5; e27
6. Kei Nagaoka, Takeo Fujiwara. Impact of Subsidies and Socioeconomic Status on Varicella Vaccination in
Greater Tokyo, Japan. Front Pediatr. 2016; 4; 19
7. Yosuke Inoue, Andrew Stickley, Aki Yazawa, Takeo Fujiwara, Katsunori Kondo, Naoki Kondo. Monthof birth is associated with mortality among older people in Japan: Findings from the JAGES cohort.
Chronobiol. Int.. 2016; 33(4); 441-447
8. Satomi Doi, Kengo Yokomitsu, Yuji Sakano. The relationships between valued action, behavioral activation, avoidance, and reinforcement in a sample of college students Psychological Reports. 2016; 118(1);5-22
9. Ayako Morita, Kazumi Natsuhara, Gwendalyn Vengiau, Cindy Chia-Jung Chen, Shingo Odani, Tsukasa
Inaoka, Kiyoshi Tadokoro, Kazuhiro Suda, Takuro Furusawa, Peter Siba, Suparat Phuanukoonnon, Masahiro
Umezaki. Reduced morning cortisol concentration in saliva was associated with obesity: Evidence from
community-dwelling adults in papua new guinea. Am. J. Hum. Biol.. 2016.01;
10. Masahiro Umezaki, Yuichi I Naito, Takumi Tsutaya, Jun Baba, Kiyoshi Tadokoro, Shingo Odani, Ayako
Morita, Kazumi Natsuhara, Suparat Phuanukoonnon, Gwendalyn Vengiau, Peter M Siba, Minoru Yoneda.
Association between sex inequality in animal protein intake and economic development in the Papua New
Guinea highlands: The carbon and nitrogen isotopic composition of scalp hair and ﬁngernail. Am. J.
Phys. Anthropol.. 2016.01; 159(1); 164-173
11. Satomi Doi. The elaboration of behavioral activation approach focusing on promoting value-based behavior 2016.03;
12. Satomi Doi, Kengo Yokomitsu, Yuji Sakano. Behavioral Activation Approach with Focus on Values : A
Meta-Analysis 2016.03; 16(2); 207-218
13. Hiroaki Honma, Makiko Okuyama, Takeo Fujiwara, Shukei Gouzu. Large-scale disasters and PTSD
in young children: A follow-up study of preschool children severely aﬀected by the Great East Japan
Earthquake Japanese Journal of Child and Adolescent Psychiatry. 2016.04; 57(2); 283-297― 125 ―

Public HealthPublic Health

14. Shihoko Koyama, Jun Aida, Masashige Saito, Naoki Kondo, Yukihiro Sato, Yusuke Matsuyama, YukakoTani, Yuri Sasaki, Katsunori Kondo, Toshiyuki Ojima, Tatsuo Yamamoto, Toru Tsuboya, Ken Osaka.
Community social capital and tooth loss in Japanese older people: a longitudinal cohort study. BMJ
Open. 2016.04; 6(4); e010768
15. Mashal MO, Nakamura K, Kizuki M. Hidden burden of non-medical spending associated with inpatientcare among the poor in Afghanistan. International Journal of Public Health. 2016.05; 61; 661-671
16. Airi Amemiya, Takeo Fujiwara. Association between maternal intimate partner violence victimization
during pregnancy and maternal abusive behavior towards infants at 4 months of age in Japan. Child
Abuse Negl. 2016.05; 55; 32-39
17. Sayaka Aoki, Keiji Hashimoto, Natsuha Ikeda, Makoto Takekoh, Takeo Fujiwara, Naho Morisaki, Hidetoshi
Mezawa, Yoshiyuki Tachibana, Yukihiro Ohya. Comparison of the Kyoto Scale of Psychological Development 2001 with the parent-rated Kinder Infant Development Scale (KIDS). Brain Dev.. 2016.05; 38(5);481-490
18. Rami Al Rifai, Keiko Nakamura, Kaoruko Seino. Decline in the prevalence of anaemia among children ofpre-school age after implementation of wheat ﬂour fortiﬁcation with multiple micronutrients in Jordan.
Public Health Nutr. 2016.06; 19; 1486-1497
19. Aya Isumi, Takeo Fujiwara. Association of adverse childhood experiences with shaking and smothering
behaviors among Japanese caregivers. Child Abuse and Neglect. 2016.07; 57; 12-20
20. Nakamura K, Chiba M, Seino K. Factors inﬂuencing reduction of risk of lifestyle related disease amongchildren. 2016.08; 31; 381-386
21. Katsura Igai, Manabu Itakura, Suguru Nishijima, Hirohito Tsurumaru, Wataru Suda, Takumi Tsutaya,
Eriko Tomitsuka, Kiyoshi Tadokoro, Jun Baba, Shingo Odani, Kazumi Natsuhara, Ayako Morita, Minoru
Yoneda, Andrew R Greenhill, Paul F Horwood, Jun-Ichi Inoue, Moriya Ohkuma, Yuichi Hongoh, Taro
Yamamoto, Peter M Siba, Masahira Hattori, Kiwamu Minamisawa, Masahiro Umezaki. Nitrogen ﬁxationand nifH diversity in human gut microbiota. Sci Rep. 2016.08; 6; 31942
22. Junko Yagi, Takeo Fujiwara, Takehito Yambe, Makiko Okuyama, Ichiro Kawachi, Akio Sakai. Does social
capital reduce child behavior problems? Results from the Great East Japan Earthquake follow-up forChildren Study. Soc Psychiatry Psychiatr Epidemiol. 2016.08; 51(8); 1117-1123
23. Nobuyuki Nosaka, Takeo Fujiwara, Emily Knaup, Ayumi Okada, Hirokazu Tsukahara. Validity of Mothers’ Reports of Children’s Weight in Japan. Acta Med. Okayama. 2016.08; 70(4); 255-259
24. Yukako Tani, Naoki Kondo, Yuiko Nagamine, Tomohiro Shinozaki, Katsunori Kondo, Ichiro Kawachi,
Takeo Fujiwara. Childhood socioeconomic disadvantage is associated with lower mortality in older
Japanese men: the JAGES cohort study. Int J Epidemiol. 2016.08; 45(4); 1226-1235
25. Manami Ochi, Takeo Fujiwara. Association Between Parental Social Interaction and Behavior Problems
in Oﬀspring: a Population-Based Study in Japan. Int J Behav Med. 2016.08; 23(4); 447-457
26. Yukako Tani, Takeo Fujiwara, Naoki Kondo, Hisashi Noma, Yuri Sasaki, Katsunori Kondo. Childhood
Socioeconomic Status and Onset of Depression among Japanese Older Adults: The JAGES Prospective
Cohort Study. Am J Geriatr Psychiatry. 2016.09; 24(9); 717-726
27. Naho Morisaki, Ichiro Kawachi, Emily Oken, Takeo Fujiwara. Parental Characteristics can Explain Why
Japanese Women Give Birth to the Smallest Infants in the United States. Paediatr Perinat Epidemiol.
2016.09; 30(5); 473-478
28. Shaﬁqullah H,Morita A,Nakamura K, Seino K . The family planning conundrum in Afghanistan Health
Promotion International. 2016.10;
29. Al-Sobaihi S, Nakamura K, Kizuki M.. Undernutrition among children under 5 years of age in Yemen: Roleof adequate childcare provided by adults under conditions of food insecurity. Journal of Rural Medicine.
2016.11; 11(2); 47-57
30. Tumurkhuu T, Fujiwara T, Komazaki Y, Kawaguchi Y, Tanaka T, Inazawa J, Ganburged G, Bazar A,
Ogawa T, Moriyama K. Association between maternal education and malocclusion in Mongolian adolescents: a cross-sectional study. BMJ Open. 2016.11; 6(11);― 126 ―

Public HealthPublic Health

31. Munehiro Furuichi, Takeo Fujiwara, Akinari Fukuda, Mureo Kasahara, Isao Miyairi. Fulminant Hepatic
Failure as a Risk Factor for Cytomegalovirus Infection in Children Receiving Preemptive Therapy After
Living Donor Liver Transplantation. Transplantation. 2016.11; 100(11); 2404-2409
32. Aki Yazawa, Yosuke Inoue, Takeo Fujiwara, Andrew Stickley, Kokoro Shirai, Airi Amemiya, Naoki Kondo,
Chiho Watanabe, Katsunori Kondo. Association between social participation and hypertension amongolder people in Japan: the JAGES Study. Hypertens. Res.. 2016.11; 39(11); 818-824
33. Hisashi Eguchi, Akihito Shimazu, Takeo Fujiwara, Noboru Iwata, Kyoko Shimada, Masaya Takahashi,
Masahito Tokita, Izumi Watai, Norito Kawakami. The eﬀects of workplace psychosocial factors on whether
Japanese dual-earner couples with preschool children have additional children: a prospective study. Ind
Health. 2016.12; 54(6); 498-504
34. Yusuke Matsuyama, Takeo Fujiwara, Jun Aida, Richard G Watt, Naoki Kondo, Tatsuo Yamamoto, Katsunori Kondo, Ken Osaka. Experience of childhood abuse and later number of remaining teeth in older
Japanese: a life-course study from Japan Gerontological Evaluation Study project. Community Dent Oral
Epidemiol. 2016.12; 44(6); 531-539[Misc]
1. Takeo Fujiwara, Naho Morisaki, Yukiko Honda, Makiko Sampei, Yukako Tani. Chemicals, Nutrition, andAutism Spectrum Disorder: A Mini-Review. Front Neurosci. 2016; 10; 174[Conference Activities & Talks]
1. Nakamura K. Post-disaster excess deaths of NCD and Disaster Health Emergency Assistance Team..
WHO Meeting on Healthy and Resilient Cities 2016.01.11 Gandong-gu, Seoul, Republic of Korea
2. Nakamura K. Healthy Cities and Food is Asia. HCSO-AFHC Symposium 2016.02.02 Tokyo
3. Nakamura K. Examples of actions to address urban health challenges: Urban health system roles andfunctions: Emergency Preparedness Plan. Meeting on Implementation of the Regional Framework for
Urban Health in the Western Paciﬁc (2016-2020) 2016.02.29 Manila, Philippines
4. Takeo Fujiwara, Yukako Tani. Does eat-vegetable-ﬁrst policy prevent children s caries?. 8th Annual
Meeting, International Society for Social Capital research 2016.05.27
5. Satomi Doi, Takeo Fujiwara. The association between leaving children home alone and mental health:Result from A-CHILD study.. The 8th Annual Meeting International Society for Social Capital research
2016.05.30
6. Kaori Honjo, Naoki Kondo, Yukako Tani, Masashige Saitoh, Katsunori Kondo. Living arrangements andrisk of depressive symptoms among older adults in Japan: The JAGES longitudinal survey. Society of
Epidemiologic Research 49th Annual Meeting 2016.06 Miami, USA
7. Aya Isumi, Takeo Fujiwara. Shaken Baby Syndrome and Social Capital: A Multilevel Analysis of Caregivers of Infants in Japan. The Society for Pediatric and Perinatal Epidemiologic Research 29th Annual
Meeting 2016.06.21 Miami, Florida, USA
8. Aya Isumi, Takeo Fujiwara. Association of Adverse Childhood Experiences with Shaking and Smothering
Behaviors among Japanese Caregivers . The 2016 Epidemiology Congress of the Americas 2016.06.22
Miami, Florida, USA
9. Takeo Fujiwara, Aya Isumi. Interaction eﬀect of unexpected pregnancy and younger motherhood on infantabuse: A retrospective cohort study in Japan. 2016.06.23
10. Satomi Doi, Kengo Yokomitsu, Koki Takagaki et al.. The eﬀect of values intervention in behavioral
activation in college students: Randomized controlled trial. The 8th World Congress of Behavioral andCognitive Therapies 2016.06.24
11. Shihoko Koyama, Jun Aida, Masashige Saito, Naoki Kondo, Yukihiro Sato, Yusuke Matsuyama, YukakoTani, Yuri Sasaki, Katsunori Kondo, Toshiyuki Ojima, Tatsuo Yamamoto, Toru Tsuboya, Ken Osaka.
Community social capital and oral health in Japanese older people. IADR PBRG Symposium 20162016.06.27 Nagoya, Japan― 127 ―

Public HealthPublic Health

12. Nakamura K. Health Supportive City Development. The 12th Annual Conference of the Japan Chapterof the Alliance for Healthy Cities 2016.07.26
13. Nakamura K. Public Health. Graduate School Seminar at School of Public Health, MUHAS 2016.08.03
Dar es Salaam, Tanzania
14. Nakamura K, Fawkes S. The Foresight Approach towards Healthy and Resilient Cities. The 7th Global
Conference of the Alliance for Healthy Cities 2016.08.29 Wonju, Republic of Korea
15. Aya Isumi, Takeo Fujiwara. Association of positive parenting practice with shaking and smothering among
caregivers of infants in Japan. 21st International Congress on Child Abuse and Neglect 2016.08.29 Calgary,
Canada
16. Nakamura K. Nationwide program for prevention and control of lifestyle related disease, Japan.. 8th NCD
Forum Seminar 2016.09.28 Seoul, Republic of Korea
17. Masashi Kizuki, Manami Ochi, Aya Isumi, Tsuguhiko Kato, Takeo Fujiwara. Association between mental
health of children and time parents return home from work. 75th Annual Meating of Japanese Society of
Public Health 2016.10.26 Osaka city, Osaka
18. Aya Isumi, Makiko Sampei, Takeo Fujiwara. The eﬀect of the educational video on infant crying on
prevention of Shaken Baby Syndrome among Japanese caregivers. 75th Annual Meeting of JapaneseSociety of Public Health 2016.10.27 Osaka city, Osaka
19. Masashi Kizuki, Takeo Fujiwara. Association between family support and psychological distress, as modiﬁed by attachment patterns. 9th European Public Health Conference 2016.11.10 Vienna, Austria
20. Aya Isumi, Takeo Fujiwara. Interaction eﬀects of unintended pregnancy and maternal age on infant abusein Japan. 9th European Public Health Conference 2016.11.12 Vienna, Austria
21. Takeo Fujiwara, Manami Ochi, Aya Isumi, Tsuguhiko Kato. Modiﬁable mediators on the association
between child poverty and health in Japan. 9th European Public Health Conference 2016.11.12
22. Aya Isumi, Manami Ochi, Tsuguhiko Kato, Takeo Fujiwara. Child maltreatment among economicallydisadvantaged families: Evidence from A-CHILD Study . 22th Annual Meeting of Japanese Society for
Prevention of Child Abuse and Neglect 2016.11.26 Osaka city, Osaka
23. Kaori Honjo, Koichiro Shiba, Yukako Tani, Yuri Sasaki, Katsunori Kondo, Naoki Kondo. The risk of depressive symptoms according to living alone and social support among Japanese older people: A ﬁxed effects analysis of longitudinal data from the Japan Gerontological Evaluation Study. ICBM2016 2016.12.10
Melbourne, Australia― 128 ―

Public Health
HealthPublicEnvironmental Parasitology

Professor Nobuo OHTA
Associate Professor Nobuaki AKAO
Lecturer Takashi KUMAGAI
Assistant Professor Akina HINO、Wataru KAGAYA (2016.4 〜)
Project Lecturer Mitsuko OHASHI (AMED J-GRID)PhD Course Students：Masafumi YAMABE, Emmanuel BLAY, Koﬁ KWOFIE,
Ripa JAMAL (〜 2016.9), Daisuke KOBAYASHI, Katsumi MAEZAWA, Nobuhide HATA,
Michael AMOA-BOSOMPEM (2016.10 〜)
Master Course Students: Junya NAKAMICHI
Research student: Kiichi YAMAMOTO(1)OutlineParasitic diseases are still serious health matters in the developing world. In spite of those situations, there
is still no enough achievement in the disease control. Moreover, recent situations such as global warming ordevelopment of rapid transportations, parasitic infections are rather spreading even in developed countries. Our
purpose of research and education are based on the global view, and comprehensive research should be done
involving development of new tools for disease control. Parasites are highly evolved animals and host-parasite
interplay is complicated. Uncovering those enigma is a big challenge for health and welfare of people all over
the world.
Purpose of our Department is developing human resources to make contributions for health and welfare of thepeople living in areas with parasitic infections.
Our Department manages the Joint research project with Noguchi Memorial Institute for Medical Research,
Ghana, supported by AMED, Japan.
(2)

Research(1) Pathological Research on zoonotic parasitoses: Toxocariasis, Diloﬁlariasis(2) Epidemiological survey of parasitic diseases: Opistorchis infection, Schistosomiasis were analyzed by the useof new diagnostic tools in SE Asian areas.
(3) Molecular epidemiology of tropical diseases: Drug resistance of Aedes mosquitoes, Molecular detection ofarthropod viruses in mosquitoes, and their metagenomic analyses..
(4) Immunopathology of schistosomiasis: Regulation of egg-granuloma formation in schistosomiasis japonica.
(5) Drug development against parasitic infection: New drug candidates for schistosomiasis were investigated fortheir target compounds.
(6) Regulation of gene expression in parasitic helminthes: RNAi and parasitism in schistosome parasites.
(7) Molecular and epidemiological research on parasitic infections and vector mosquitoes in West African subregion.
(3)

Education

Because of the recent development and the global changes in social system and life style, parasitic infections
are becoming more heterogeneous. When we consider about factors promoting spreading parasitic diseases,
multidisciplinary approaches are needed: medical, zoological and sociological.

― 129 ―

Public HealthPublicHealth

Our laboratory mainly deals with pathophysiology and epidemiology of parasitic infections. Immunology,
molecular biology and clinico-pathology are approaches employed. Main subjects in our laboratory are schistosomiasis, zoonotic helminthiases, malaria and trypanosomiasis, all of which include laboratory and ﬁeld investigations. In the schistosomiasis research, we focus on developing new diagnostic method by DNA detection in
the sample, and uncovering immunopathogenesis of the typical hepatic inﬂammation. For zoonotic helminthiases, developments of diagnostic tools are urgent matters to be studied. Our laboratory is one of the reference
stations for the diagnosis in Japan.
Since 2008, TMDU started collaboration project on research on infectious diseases at Noguchi Memorial Institute for Medical Research. At the collaboration center at NMIMR, molecular approaches to discover new drug
targets for African trypanosomiasis are underway.
Our Department managed Public Health Medicine Course under the special grant from Ministry of Education,
Culture, Sports, Science and Technology, Japan.
(4)

Lectures & Courses

Lecture and practices of basic and clinical parasitology are given. Further more, Field practice is importantfor future career. It is important to have ﬁeld experiences where each student ﬁnd matters and problems to beclariﬁed. Together with those, ﬁnal goal is to develop human resources with enough knowledge and experiences.
(5)

Clinical Services & Other Works

Clinical services for the diagnosis of parasitic infections are our routine activities. Furthermore, epidemiologicalsurveillance and disease control activities in the endemic ﬁelds are intended to enhance health and welfare ofresidents.
(6)

Publications[Original Articles]
1. Ikeuchi N, Itoi T, Tonozuka R, Mukai S, Koyama Y, Tsuchiya T, Matsumoto T, Mizuno Y, Nakamura
I, Hino A, Moriyasu F. Strongyloides stercoralis Infection Causing Obstructive Jaundice and Refractory
Pancreatitis: A Lesson Learned from a Case Study. Internal Medicine. 2016; 55(15); 2081-2086
2. Hino A, Maruyama H, Kikuchi T.. A novel method to assess the biodiversity of parasites using 18S rDNA
Illumina sequencing; parasitome analysis method. Parasitology international. 2016.01;
3. Ayi I, Kwoﬁe KD, Blay EA, Osei JH, Frempong KK, Koku R, Ghansah A, Lartey M, Suzuki T, Boakye
DA, Koram KA, Ohta N.. Clonal types of Toxoplasma gondii among immune compromised and immune
competent individuals in Accra, Ghana. Parasitology International. 2016.06; 65(3); 238-244
4. Motomura A, Norose K, Hoshioka Y, Torimitsu S, Chiba F, Makino Y, Inokuchi G, Yajima D, Ohta
N, Kumagai T, Iwase H.. Forensic study of Schistosoma japonicum eggs found in an autopsy case.
Parasitology International. 2016.06; 65(3); 285-287
5. Ayumi Motomura, Kazumi Norose, Yumi Hoshioka, Suguru Torimitsu, Fumiko Chiba, Yohsuke Makino,
Go Inokuchi, Daisuke Yajima, Nobuo Ohta, Takashi Kumagai, Hirotaro Iwase. Forensic study of Schistosoma japonicum eggs found in an autopsy case. Parasitol. Int.. 2016.06; 65(3); 285-287
6. Koﬁ D Kwoﬁe, Nguyen Huu Tung, Mitsuko Suzuki-Ohashi, Michael Amoa-Bosompem, Richard Adegle,
Maxwell M Sakyiamah, Frederick Ayertey, Koﬁ Baﬀour-Awuah Owusu, Isaac Tuﬀour, Philip Atchoglo,
Kwadwo K Frempong, William K Anyan, Takuhiro Uto, Osamu Morinaga, Taizo Yamashita, Frederic
Aboagye, Alfred A Appiah, Regina Appiah-Opong, Alexander K Nyarko, Yasuchika Yamaguchi, Dominic
Edoh, Kwadwo A Koram, Shoji Yamaoka, Daniel A Boakye, Nobuo Ohta, Yukihiro Shoyama, Irene Ayi.
Antitrypanosomal Activities and Mechanisms of Action of Novel Tetracyclic Iridoids from Morinda lucida
Benth. Antimicrob. Agents Chemother.. 2016.06; 60(6); 3283-3290
7. Kawada H, Higa Y, Futami K, Muranami Y, Kawashima E, Osei JH, Sakyi KY, Dadzie S, de Souza DK,
Appau M, Ohta N, Suzuki T, Minakawa T.. Discovery of point mutations in the voltage-gated sodium
channel from African Aedes aegypti populations: Potential phylogetic reasons for gene introgression. PLoS
Neglected Tropical Diseases. 2016.06; 15(6); e0004780― 130 ―

Public Health
HealthPublic8. Ayi I, Sowah AO, Blay EA, Suzuki T, Ohta N, Aye-Kumi PF.. Toxoplasma gondii infections among
pregnant women, children and HIV infected persons in Accra, Ghana. Tropical Medicine and Health.
2016.06; 44(1); 17-24
9. Suzuki T, Osei JH, Sasaki A, Adimazoya M, Appau M, Boakye DA, Ohta N, Dadzie S.. Risk of transmission of viral haemorrhagic fevers and the insecticide susceptibility status of Aedes aegypti (lLinaeus)
in some sites in Accra, Ghana. Ghana Medical Journal. 2016.06; 50(3); 136-141
10. Amoa-Bosompem M, Ohashi M, Mosore MT, Agyapong J, Tung NH, Kwoﬁe KD, Ayetey F, Owusu KBA,
Tuﬀour I, Achogle P, Djameh GI, Azerygyik FA, Botchie SK, Anyan WK, Appiah-Opong R, Uto T,
Morinaga O, Appiah FA, Ayu I, Shoyama M, Ohta N.. In vitro anti-leishmania activity of tetracyclic
iridoids from Morindca lucida benth. Tropical Medicine and Health. 2016.08; 44(1); 25
11. Jamal R, Shimogawara R, Yamamoto K, Ohta N.. Anti-trypanosome eﬀects of nutritional supplements
and vitamin D3: in vitro and in vivo eﬃcacy against Trypanosoma brucei brucei. Tropical Medicine and
Health. 2016.08; 44(2); 26
12. Taisei Kikuchi, Akina Hino, Teruhisa Tanaka, Myo Pa Pa Thet Hnin Htwe Aung, Tanzila Afrin, Eiji
Nagayasu, Ryusei Tanaka, Miwa Higashiarakawa, Kyu Kyu Win, Tetsuo Hirata, Wah Win Htike, Jiro
Fujita, Haruhiko Maruyama. Genome-Wide Analyses of Individual Strongyloides stercoralis (Nematoda:
Rhabditoidea) Provide Insights into Population Structure and Reproductive Life Cycles. PLoS Neglected
Tropical Diseases. 2016.12; 10(12); e0005253
13. Kwoﬁe KD, Ghansah A, Osei JH, Frempong KK, Obed S, Frimpong EH, Boakye DA, Suzuki T, Ohta N,
Ayi I.. Indication of risk of mother-to-child Toxoplasma gondii transmission in the Greater Accra regionof Ghana. Maternal and Child Health Journal. 2016.12; 20(12); 2581-2588
14. Kobayashi D, Isawa H, Ejiri H, Sasaki T, Sunahara T, Futami K, Tsuda Y, Katayama Y, Mizutani T,
Minakawa N, Ohta N, Sawabe K.. Complete genome sequencing and phylogenetic analysis of a Getah
virus strain (Genus Alphavirus, Family Togaviridae) isolated from Culex tritaeniorhynchus mosquitoes in
Nagasaki, Japan in 2012. Vector Borne Zoonotic Diseases. 2016.12; 16(12); 769-776[Books etc]
1. Nobuo Ohta. Current Therapy 2016. Igaku Shoin Co., 2016.01[Conference Activities & Talks]
1. Takashi Kumagai, Koichiro Ichimura, Masafumi Yamabe, Rieko Shimogawara, Nobuo Ohta. Femalebiased miRNAs productions through the extracellular vesicles induced by the erythrocyte uptake in theadult worms of Schistosoma japonicum. U.S.-Japan Cooperative Medical Sciences Program (USJCMSP)Parasitic Disease Panel 2016.01.14 Bethesda, Maryland, USA
2. Akina Hino, Taisei Kikuchi, Kosuke Tanimoto, Chinami Sonobe, Takashi Kumagai, Yasuhide Saitoh,
Rieko Shimogawara, Haruhiko Maruyama, Nobuo Ohta. Phylogenetic analysis of Oncomelania spp. ,intermediate snails of Schistosoma japonicum, with whole mitochondria genome. The 85th Annual meetingof the Japanese society of Parasitology 2016.03.19
3. Wataru Kagaya, Chim Chan, James Kongere, Jesse Gitaka, Lucy Okell, Akira Kaneko. Mass drug
administration to eliminate malaria on Ngodhe Island in Lake Victoria, Kenya: a pilot study. RSTMH
Biennial Meeting - Challenges in Disease Elimination 2016.09.15 Cambridge
4. Takashi Kumagai, Moritoshi Iwagami, Masafumi Yamabe, Sengdeuane Keomalaphet, Phonepadith Khattignavong, Lavy Lorphacan, Pheovaly Soundala, Bouasy Hongvanthong, Nobuo Ohta, Paul T. Brey,
Shigeyuki Kano. The application of the LAMP method to Opisthorchis viverrini and Schistosoma mekongi
infections in Champasak Province, southern Lao PDR.. The 10th National Health Research Forum2016.10.27 Savannakhet Province, Lao PDR― 131 ―

Public HealthPublic HealthForensic Medicine

Professor
Koichi UEMURA
Associate Professor
Toshihiko AKI
Junior Associate Professor
Kana UNUMA
Assistant Professor
Takeshi FUNAKOSHI
Specially Appointed Assistant Professor
Kanako NORITAKE
Graduate Student
Atsushi YAMADA
Izumi FUNAKOSHI
Naho HIRAYAMA
Yusuke FUJIIHaruka KOJIMA
Ryo WATANABE
Rina KASEDA
Tomomi SANO
(1)

ResearchResearch Subjects
1) Toxicology2) Alcohol medicine
3) Forensic pathology(2)

Education

Purpose of education
Forensic medicine provides fundamental human rights, public safety and nation s welfare to make a fairjudgment on the items on the law which requires the medical knowledge. Education of forensic medicine isincluded forensic medicine in a narrow sense and medical law. Purpose of education in forensic medicine is toprovide students opportunity to study the essential knowledge of the relationship between medical and society(include law, ethics, suit and administration). Students are also taught a blood type and an alcohol medicine― 132 ―

Public HealthPublic Healthin a practical training.

(3)

Clinical Services & Other Works

Practical services
Forensic Medicine provides the expert opinion on a living body and a corpse to clarify causes of wound anddeath, mainly entrusted by a public prosecutor or the police, thereby, contributing fair trial in a court.

(4)

Publications[Original Articles]
1. Watanabe M, Unuma K, Makino Y, Komatsu A, Yamada A, Nagai H, Uemura K. An Autopsy Case of
Asphyxia Caused by Uncontrolled Hemorrhage Following Transbronchial Lung Biopsy J Clin Respir Dis
Care. 2016;
2. Yusuke Fujii, Takeshi Funakoshi, Kana Unuma, Kanako Noritake, Toshihiko Aki, Koichi Uemura. Hydrogen sulﬁde donor NaHS induces death of alveolar epithelial L2 cells that is associated with cellularshrinkage, transgelin expression and myosin phosphorylation. J Toxicol Sci. 2016; 41(5); 645-654
3. Haruka Kimura-Kojima, Kana Unuma, Takeshi Funakoshi, Chizuru Kato, Ayumi Komatsu, Toshihiko
Aki, Koichi Uemura. Increased MFG-E8 expression and its implications in the vascular pathophysiology
of cocaine abuse. J Toxicol Pathol. 2016.04; 29(2); 131-138
4. Takeshi Funakoshi, Toshihiko Aki, Masateru Tajiri, Kana Unuma, Koichi Uemura. Necroptosis-like Neuronal Cell Death Caused by Cellular Cholesterol Accumulation. J. Biol. Chem.. 2016.11; 291(48);25050-25065― 133 ―

Public HealthPublic Health

Health Care Management and Planning

Professor
Kazuo KAWAHARA
Assistant Professor
Makiko SUGAWA
Graduate Student Jian CHEN
Woonkwan HYUN
Masakazu KIKUCHI
Yoko KOMURA
Daisuke KUMAZAWA
Masao MURATA
Hisashi OMOTE
Masataka YANO
Hayato TAKAYAMA
Ritsuki NEGISHI
Katsunori OHOYAMA(1)OutlineBy analyzing the Japanese healthcare policies and system and by reviewing their interaction with society, thestructural characteristics and issues can be clariﬁed. To resolve or ﬁnd better ways to handle these issues, we
conduct research into public health and welfare, and its related disciplinary areas. With the cooperation ofactive policy makers and personnel from the healthcare departments, the research results can be applied to thepresent healthcare policies and system. Through this education on collecting data, clarifying issues, analyzing
the situation, and evaluating options, students taking this course are expected to grow in their ability to makehealthcare policies.
(2)

Research

In the academic areas mentioned above, we conduct research under the following topic areas:
1) The signiﬁcance of public healthcare planning, its challenges, and inﬂuences on the healthcare system
We conduct research on issues related to new healthcare policies including planning, analysis, issue resolution,and making positive changes to the healthcare plan. This research area includes the Japanese emergency medical
service and the impartial evaluation of the travel distance of aid agents and the time required for them to reach
their destination.
2) Structural choices concerning national blood services
In Japan, we experienced HIV infection from tainted blood products. There were various causes for thisevent, and improvements are required in all processes: collecting blood, screening blood, manufacturing blood
products, and following-up on the usage of these products. By analyzing background information related to theadverse events and their causes, we can propose the most appropriate policies related to blood services, thus
ensuring safety, and securing a stable supply. To achieve a stable supply of blood products, we also conduct
epidemiological studies to review guidelines on collecting blood.
3)

The government role in preventing medical errors― 134 ―

Public HealthPublic Health

Issues related to medical errors and adverse events have recently attracted a great deal of attention in Japan.
We study the role that the government should play regarding various medical errors and their prevention as
well as review and address the ﬁnancial loss caused by blood-related adverse events and policies on prevention.
4)
Structural analyses of healthcare system in the community
By reviewing and analyzing activities related to disease prevention and health promotion conducted by local
healthcare centers, we research the role of the local healthcare system and its eﬀectiveness and eﬃciency.
5) Systemizing and evaluating public health policies
We review the processes of creating public health policies and systems, address the association with the creatingprocesses and stakeholders such as political parties and lobby groups, evaluate their policies, and then suggest
improvements to these policies and systems.
6) The role of healthcare communication to ﬁll in gaps between medical providers and patients, and toshare the uncertainties related to medicine and healthcare
7)

The inﬂuence of healthcare communication on patient and medial safety

8) Reviewing communication tools and skills, and their systematic introduction into the healthcare
system in order to realize patient participation and proactive involvement in treatment processes(3)

Publications[Original Articles]
1. Takamichi Kogure, Masahiko Sumitani, Hiroaki Abe, Jun Hozumi, Reo Inoue, Kazuhito Mietani, Kazuo
Kawahara, and Yoshitsugu Yamada. . Ischemic Ulcer Pain Is Both Nociceptive and Neuropathic Pain
Based on a Discriminant Function Analysis Using the McGill Pain Questionnaire Journal of Pain& Palliative Care Pharmacotherapy. 2017.04; 0(0); 1-7― 135 ―

Public Health

Medical Genomics

Molecular Epidemiology

Professor: Masaaki MURAMATSU
Associate Professor : Noriko SATO
Assistant Professor : Chihiro Imai
Adjunct Instructor : Katsuko SUDO, Jun-ichi TAGUCHI

Graduate Student: Kaung Si Thu,
Khin Thet Thet Zaw, Yuko Maeda, Fujitani,
Tay Zar Kyaw, Tadaaki Katsuta, Jyun-ya Hagiwara, Shilpa Pavethynath
Norihiko Satake, Maidina Abudushataer, Ake Ko Ko Minn, Zong Yuan, Kenji Suzuki, Hirokazu Sakamoto, Yuiri Tsubota
Research Student: Tong Daike(1)

OutlineMany common chronic diseases are multifactorial in that they are caused by multiple genetic and environmental
factors. By applying the technology and information of human genome to epidemiological studies, we aim toclarify the role of genetic polymorphisms, epigenetic changes, as well as their interaction with environmental
factors, which may contribute to the development of these diseases.

(2)

Research

Our research subjects are as follows.
1. Gene-environment interaction that aﬀects the onset of metabolic syndrome and its related phenotypes.
2. Genetic factors that aﬀect the severity of pathological atherosclerosis.
3. Severe cutaneous adverse response (Stevens-Jhonson s Syndrome) and HLA genotypes.
4. The role of epigenetic regulation and fetal programming in common diseases.
5. Application of personal genome to preemptive & preventive medicine.

(3)

Education

Masaaki Muramatsu:Holistic Study of Disease Prevention Ⅰ
Masaaki Muramatsu:Environmental/Social Health
Masaaki Muramatsu:Negotiation and Debate in English
Noriko Sato, Masaaki Muramatsu: Bioscience I
Noriko Sato: Molecular and Cellular Biology― 136 ―

Public Health

Medical Genomics(4)

Lectures & Courses

We focus on common diseases such as diabetes, hypertension, obesity, metabolic syndrome, and atherosclerosis
which are caused by multiple genetic and environmental factors, and aim to decipher these factors as well as
their interactions by applying the technology and information of human genome to epidemiology. Our goal is
not only to identify disease genes and polymorphisms but also to elucidate gene-environment interactions that
contribute to the onset and progression of the diseases. Epigenetic changes in common diseases are also in
our scope. A new project has been started to study methods for educating genome-based health literacy byemploying information generated from personal genome sequences.
(5)

Publications[Original Articles]
1. Kumpei Tanisawa, Yasumichi Arai, Nobuyoshi Hirose, Hiroshi Shimokata, Yoshiji Yamada, Hisashi Kawai,
Motonaga Kojima, Shuichi Obuchi, Hirohiko Hirano, Hideyo Yoshida, Hiroyuki Suzuki, Yoshinori Fujiwara, Kazushige Ihara, Maki Sugaya, Tomio Arai, Seijiro Mori, Motoji Sawabe, Noriko Sato, Masaaki
Muramatsu, Mitsuru Higuchi, Yao-Wen Liu, Qing-Peng Kong, Masashi Tanaka. Exome-wide Association
Study Identiﬁes CLEC3B Missense Variant p.S106G as Being Associated With Extreme Longevity in East
Asian Populations. J. Gerontol. A Biol. Sci. Med. Sci.. 2016.05; 72(3); 309-318
2. Kaung Si Thu, Noriko Sato, Shinobu Ikeda, Makiko Naka-Mieno, Tomio Arai, Seijiro Mori, Motoji Sawabe,
Masaaki Muramatsu, Masashi Tanaka. Association of polymorphisms of the transporter associated with
antigen processing (TAP2) gene with pulmonary tuberculosis in an elderly Japanese population. APMIS.
2016.08; 124(8); 675-680
3. Heying Zhou, Seijiro Mori, Tatsuro Ishizaki, Masashi Tanaka, Kumpei Tanisawa, Makiko Naka Mieno,
Motoji Sawabe, Tomio Arai, Masaaki Muramatsu, Yoshiji Yamada, Hideki Ito. Genetic risk score basedon the lifetime prevalence of femoral fracture in 924 consecutive autopsies of Japanese males. J. Bone
Miner. Metab.. 2016.11; 34(6); 685-691
4. Khin Thet Thet Zaw, Noriko Sato, Shinobu Ikeda, Kaung Si Thu, Makiko Naka Mieno, Tomio Arai, Seijiro
Mori, Tetsushi Furukawa, Tetsuo Sasano, Motoji Sawabe, Masashi Tanaka, Masaaki Muramatsu. Association of ZFHX3 gene variation with atrial ﬁbrillation, cerebral infarction, and lung thromboembolism:
An autopsy study. J Cardiol. 2016.12; 70(2); 180-184
5. Yuko Maeda, Noriko Sato, Makiko Naka-Mieno, Seijiro Mori, Tomio Arai, Masashi Tanaka, Masaaki
Muramatsu, Motoji Sawabe. Association of non-synonymous variants in WIPF3 and LIPA genes withabdominal aortic aneurysm: an autopsy study. J Geriatr Cardiol. 2016.12; 13(12); 960-967[Conference Activities & Talks]
1. Noriko Sato, Hidemi Takimoto, Tay Zar Kyaw, Nay Chi Htun, Chihiro Imai, Asako Igarashi,Yuiri Tsubota,
Reiko Tajirika-Shirai, Satoshi Yago, Tomoko Aoyama, Motoko Okamitsu, Naoyuki Miyasaka. Cohort
proﬁle: Birth Cohort – Gene and Environment Interaction Study of TMDU (BC-GENIST). The 5th Annual
Meeting of the Japan Society for Developmental Origins of Health and Disease
2. Noriko Sato. To understand the mechanisms by which metabolic disease develop due to antenatal nutritional environment . The 63rd meeting for the Japanese society of nutrition and dietetics 2016.09.08
3. Kaung Si Thu, Noriko Sato, Shinobu Ikeda, Makiko Naka-Mieno, Tomio Arai, Seijiro Mori, Motoji Sawabe,
Masashi Tanaka, Masaaki Muramatsu. Association of polymorphisms of the transporter associated with
antigen processing (TAP2) gene with pulmonary tuberculosis in an elderly Japanese population. The
Molecular Biology Society of Japan 2016 2016.12.02 Yokohama
4. Khin Thet Thet Zaw, Noriko Sato, Shinobu Ikeda, Kaung Si Thu, Makiko Naka-Mieno, Tomio Arai,
Seijiro Mori, Tetsushi Furakawa, Tetsuo Sasano, Motoji Sawabe, Masashi Tanaka, Masaaki Muramatsu.
Phenome scan of ZFHX3 gene variation: association with atrial ﬁbrillation, cerebral infarction, and lungthromboembolism. The Molecular Biology Society of Japan 2016 2016.12.02― 137 ―

Public HealthPublic HealthResearch Development

Faculty Staﬀ
Professor
Kozo TAKASE
Graduate Students
Doctor course
Akemi HIRABAYASHI
Tomoko IZUGAMI
Akira MIURA
Hidehiro ANDO
Yasumasa OOSHIRO
Hideki TERUYA
Masakazu HARAMO
Rinshuu SHIMABUKURO
Kazushige ENDOH
Master course (Master of Medical Administration)
Ai SONODA
Yoshimi NAKANISHI
Daisuke NAMEKI
Jun HATTORI
Satoshi YABE
Atsushi YOSHIDA(1)

Research

1) Introduction of Clinical Pathway in hospital2) Medical law suit and professional information
3) Quality management of medical law suit4) Organizational logic for hospital5) Health care policy and rational
6) Management of medical information and privacy7) Hospitality in medicine
8) Clinical guideline and medical quality
9) Development of medical engineering apparatus(2)

Education

1) Hospital Information Management
2) Medical Informatics, statistics
3) TQM in medicine
4) Biological bias and data management5) Medical Law and Ethics― 138 ―

Public HealthPublic Health6) Medical induction course for Judges and Prosecutors (collaborated with the Supreme Court and Departmentof Justice)7) Medical Engineering special program with Tokyo Institute of Technology8) Health Promotion Policy program (General Medicine, Risk Management in Medicine) with Hitotsubashi
University(3)

Lectures & Courses

Study on development of medical system and hospital management
Goals/outline:
The goals supposed in the lecture are mastering the technique of implementation of research development
and acquiring the ability of management of projects.
(4)

Clinical Services & Other Works

Kozo TAKASE
Committee member of Legal Training for Judicial Apprentice, Japanese Supreme Court
Chief Editorial Board of Japanese Society for Clinical Pathway(5)

Publications― 139 ―

Public HealthPublic Health

Health Policy and Informatics

Professor:Kiyohide FUSHIMI
Graduate Student:Asako TUKASAKI, kyoko SHINODA,
Ayako MATSUO, Motoko TAIMA(SANO), Toshihiro TAMAKI,
Yuya MIZUNO, Hiroki AIZAWA, Tetu OHNUMA, Akira HOMMA,
Eishi UECHI, Nobuo SAKATA, Tomomitu ICHIKAWA,
Mariko KODAN, Norihiko INOUE, Kyoko HIRANO, Mihoko OTA,
Ken KAWASAKI, Natsuko KANAZAWA, Shunsuke EDAKUBO,
Yoshiteru YANO, Yoko NUKAYA
Graduate Research Student:Masahiro INOUE(1)

Research

1) Functional diﬀerentiation and coordination of healthcare facilities
2) Development and application of patient case mix system for Japanese healthcare settings3) Application of information technology to standardization of health care and sharing of health care information.

(2)

Education

Health policy informatics is a branch of health policy science which deals with the application of information
technology to health policy research. Main objective of health policy informatics in the graduate course isto acquire ability to independently design, manage and accomplish researches in health policy and healthinformatics ﬁelds.

(3)

Publications[Original Articles]
1. So Kato, Hirotaka Chikuda, Junichi Ohya, Takeshi Oichi, Hiroki Matsui, Kiyohide Fushimi, Katsushi
Takeshita, Sakae Tanaka, Hideo Yasunaga. Risk of infectious complications associated with blood transfusion in elective spinal surgery-a propensity score matched analysis. Spine J. 2016.01; 16(1); 55-60
2. Takashi Tagami, Hiroki Matsui, Kiyohide Fushimi, Hideo Yasunaga. Prophylactic Antibiotics May Improve Outcome in Patients With Severe Burns Requiring Mechanical Ventilation: Propensity Score Analysis of a Japanese Nationwide Database. Clin. Infect. Dis.. 2016.01; 62(1); 60-66
3. Tomoki Wada, Hideo Yasunaga, Hiromasa Horiguchi, Takehiro Matsubara, Kiyohide Fushimi, Susumu
Nakajima, Naoki Yahagi. Outcomes of Argatroban Treatment in Patients With Atherothrombotic Stroke:
Observational Nationwide Study in Japan. Stroke. 2016.02; 47(2); 471-476
4. Sayaka Suzuki, Hideo Yasunaga, Hiroki Matsui, Kiyohide Fushimi, Yuki Saito, Tatsuya Yamasoba. Factors
Associated With Neck Hematoma After Thyroidectomy: A Retrospective Analysis Using a Japanese
Inpatient Database. Medicine (Baltimore). 2016.02; 95(7); e2812― 140 ―

Public HealthPublic Health

5. Junichi Ohya, Yasushi Oshima, Hirotaka Chikuda, Takeshi Oichi, Hiroki Matsui, Kiyohide Fushimi, Sakae
Tanaka, Hideo Yasunaga. Does the microendoscopic technique reduce mortality and major complications
in patients undergoing lumbar discectomy? A propensity score-matched analysis using a nationwide
administrative database. Neurosurg Focus. 2016.02; 40(2); E5
6. Tsuyoshi Hamada, Hideo Yasunaga, Yousuke Nakai, Hiroyuki Isayama, Hiroki Matsui, Kiyohide Fushimi,
Kazuhiko Koike. Interstitial lung disease associated with gemcitabine: A Japanese retrospective cohort
study. Respirology. 2016.02; 21(2); 338-343
7. Masao Iwagami, Hideo Yasunaga, Eisei Noiri, Hiromasa Horiguchi, Kiyohide Fushimi, Takehiro Matsubara, Naoki Yahagi, Masaomi Nangaku, Kent Doi. Potential Survival Beneﬁt of Polymyxin B Hemoperfusion in Septic Shock Patients on Continuous Renal Replacement Therapy: A Propensity-Matched
Analysis. Blood Purif.. 2016.02; 42(1); 9-17
8. Hanako Ishikawa, Hideo Yasunaga, Hiroki Matsui, Kiyohide Fushimi, Norito Kawakami. Diﬀerences
in cancer stage, treatment and in-hospital mortality between patients with and without schizophrenia:
retrospective matched-pair cohort study. Br J Psychiatry. 2016.03; 208(3); 239-244
9. Takashi Tagami, Hiroki Matsui, Kiyohide Fushimi, Hideo Yasunaga. Changes in Therapeutic Hypothermia
and Coronary Intervention Provision and In-Hospital Mortality of Patients With Out-of-Hospital Cardiac
Arrest: A Nationwide Database Study. Crit. Care Med.. 2016.03; 44(3); 488-495
10. Yusuke Tsuda, Hideo Yasunaga, Hiromasa Horiguchi, Kiyohide Fushimi, Hirotaka Kawano, Sakae Tanaka.
Complications and Postoperative Mortality Rate After Surgery for Pathological Femur Fracture Related
to Bone Metastasis: Analysis of a Nationwide Database. Ann. Surg. Oncol.. 2016.03; 23(3); 801-810
11. Ryo Momosaki, Hideo Yasunaga, Wataru Kakuda, Hiroki Matsui, Kiyohide Fushimi, Masahiro Abo. Very
Early versus Delayed Rehabilitation for Acute Ischemic Stroke Patients with Intravenous Recombinant
Tissue Plasminogen Activator: A Nationwide Retrospective Cohort Study. Cerebrovasc. Dis.. 2016.03;
42(1-2); 41-48
12. Sayaka Suzuki, Hideo Yasunaga, Hiroki Matsui, Hiromasa Horiguchi, Kiyohide Fushimi, Tatsuya Yamasoba. Pharyngocutaneous ﬁstula and delay in free oral feeding after pharyngolaryngectomy for hypopharyngeal cancer. Head Neck. 2016.04; 38(S1); E625-E630
13. Shotaro Aso, Hiroki Matsui, Kiyohide Fushimi, Hideo Yasunaga. In-hospital mortality and successful
weaning from venoarterial extracorporeal membrane oxygenation: analysis of 5,263 patients using a national inpatient database in Japan. Crit Care. 2016.04; 20(1); 80
14. Y Sasabuchi, H Yasunaga, H Matsui, A K Lefor, K Fushimi. Prolonged propofol infusion for mechanicallyventilated children. Anaesthesia. 2016.04; 71(4); 424-428
15. Momosaki Ryo, Yasunaga Hideo, Matsui Hiroki, Horiguchi Hiromasa, Fushimi Kiyohide, Abo Masahiro.
Predictive factors for oral intake after aspiration pneumonia in older adults. Geriatr Gerontol Int. 2016.05;
16(5); 556-560
16. Toshiaki Isogai, Hideo Yasunaga, Hiroki Matsui, Hiroyuki Tanaka, Kiyohide Fushimi. Relationship between hospital volume and major cardiac complications of rotational atherectomy: A nationwide retrospective cohort study in Japan. J Cardiol. 2016.05; 67(5); 442-448
17. Ryo Momosaki, Hideo Yasunaga, Hiroki Matsui, Kiyohide Fushimi, Masahiro Abo. Proton Pump Inhibitors versus Histamine-2 Receptor Antagonists and Risk of Pneumonia in Patients with Acute Stroke.
J Stroke Cerebrovasc Dis. 2016.05; 25(5); 1035-1040
18. Sachiko Ono, Miho Ishimaru, Yosuke Ono, Hiroki Matsui, Kiyohide Fushimi, Hideo Yasunaga. Impact
of Body Mass Index on the Outcomes of Open Reduction for Mandibular Fractures. J. Oral Maxillofac.
Surg.. 2016.05; 74(5); 1024:e1-1024:e5
19. Junko Hirashima, Hayato Yamana, Hiroki Matsui, Kiyohide Fushimi, Hideo Yasunaga. Eﬀect of intravenous magnesium sulfate on mortality in patients with severe acute asthma. Respirology. 2016.05; 21(4);668-673
20. Michio Naganuma, Hiroki Matsui, Kiyohide Fushimi, Hideo Yasunaga. Clinical features of isolated dissections of abdominal aortic branches. Heart Vessels. 2016.06; 31(6); 1006-1009― 141 ―

Public HealthPublic Health21. Miho Ishimaru, Sachiko Ono, Sayaka Suzuki, Hiroki Matsui, Kiyohide Fushimi, Hideo Yasunaga. Risk
Factors for Free Flap Failure in 2,846 Patients With Head and Neck Cancer: A National Database Studyin Japan. J. Oral Maxillofac. Surg.. 2016.06; 74(6); 1265-1270
22. Yutaka Yamaji, Hideo Yasunaga, Yoshihiro Hirata, Atsuo Yamada, Shuntaro Yoshida, Hiromasa Horiguchi,
Kiyohide Fushimi, Kazuhiko Koike. Association Between Colorectal Cancer and Atherosclerotic Diseases:
A Study Using a National Inpatient Database in Japan. Dig. Dis. Sci.. 2016.06; 61(6); 1677-1685
23. Yasuhiro Yamauchi, Hideo Yasunaga, Wakae Hasegawa, Yukiyo Sakamoto, Hideyuki Takeshima, Taisuke
Jo, Hiroki Matsui, Kiyohide Fushimi, Takahide Nagase. Eﬀect of outpatient therapy with inhaled corticosteroids on decreasing in-hospital mortality from pneumonia in patients with COPD. Int J Chron Obstruct
Pulmon Dis. 2016.06; 11; 1403-1411
24. Keishi Oda, Kazuhiro Yatera, Yoshihisa Fujino, Hiroshi Ishimoto, Hiroyuki Nakao, Tetsuya Hanaka,
Takaaki Ogoshi, Takashi Kido, Kiyohide Fushimi, Shinya Matsuda, Hiroshi Mukae. Eﬃcacy of concurrent
treatments in idiopathic pulmonary ﬁbrosis patients with a rapid progression of respiratory failure: ananalysis of a national administrative database in Japan. BMC Pulm Med. 2016.06; 16(1); 91
25. Toshiaki Isogai, Hiroki Matsui, Hiroyuki Tanaka, Kiyohide Fushimi, Hideo Yasunaga. Early β-blocker use
and in-hospital mortality in patients with Takotsubo cardiomyopathy. Heart. 2016.07; 102(13); 1029-1035
26. Yusuke Sasabuchi, Hiroki Matsui, Alan K Lefor, Kiyohide Fushimi, Hideo Yasunaga. Risks and Beneﬁts
of Stress Ulcer Prophylaxis for Patients With Severe Sepsis. Crit. Care Med.. 2016.07; 44(7); e464-e469
27. Hironori Uematsu, Susumu Kunisawa, Kazuto Yamashita, Kiyohide Fushimi, Yuichi Imanaka. Impact ofweekend admission on in-hospital mortality in severe community-acquired pneumonia patients in Japan.
Respirology. 2016.07; 21(5); 905-910
28. Sayaka Suzuki, Hideo Yasunaga, Hiroki Matsui, Kiyohide Fushimi, Tatsuya Yamasoba. Factors associatedwith prolonged duration of post-tympanoplasty local treatment in adult chronic otitis media patients: A
retrospective observational study using a Japanese inpatient database. Auris Nasus Larynx. 2016.08;
43(4); 377-381
29. Tetsu Ohnuma, Daisuke Shinjo, Kiyohide Fushimi. Hospital mortality of patients aged 80 and older after
surgical repair for type A acute aortic dissection in Japan. Medicine (Baltimore). 2016.08; 95(31); e4408
30. Sayaka Suzuki, Hideo Yasunaga, Hiroki Matsui, Kiyohide Fushimi, Yuki Saito, Tatsuya Yamasoba. Cerebral infarction after intraarterial and intravenous chemoradiotherapy for head and neck cancer: A retrospective analysis using a Japanese inpatient database. Head Neck. 2016.09; 38(9); 1354-1358
31. Yasuhiro Yamauchi, Hideo Yasunaga, Yukiyo Sakamoto, Wakae Hasegawa, Hideyuki Takeshima, Hirokazu
Urushiyama, Taisuke Jo, Hiroki Matsui, Kiyohide Fushimi, Takahide Nagase. Mortality associated withbone fractures in COPD patients. Int J Chron Obstruct Pulmon Dis. 2016.09; 11; 2335-2340
32. Seiko Mizuno, Susumu Kunisawa, Noriko Sasaki, Kiyohide Fushimi, Yuichi Imanaka. In-hospital mortality and treatment patterns in acute myocardial infarction patients admitted during national cardiology
meeting dates. Int. J. Cardiol.. 2016.10; 220; 929-936
33. Nobuo Sakata, Sayuri Shimizu, Fumio Hirano, Kiyohide Fushimi. Epidemiological study of adult-onset
Still’s disease using a Japanese administrative database. Rheumatol. Int.. 2016.10; 36(10); 1399-1405
34. Hayato Yamana, Hiroki Matsui, Takashi Tagami, Junko Hirashima, Kiyohide Fushimi, Hideo Yasunaga.
De-escalation versus continuation of empirical antimicrobial therapy in community-acquired pneumonia.
J. Infect.. 2016.10; 73(4); 314-325
35. Hayato Yamana, Hiromasa Horiguchi, Kiyohide Fushimi, Hideo Yasunaga. Comparison of ProcedureBased and Diagnosis-Based Identiﬁcations of Severe Sepsis and Disseminated Intravascular Coagulationin Administrative Data. J Epidemiol. 2016.10; 26(10); 530-537
36. Takumi Matsumoto, Hideo Yasunaga, Hiroki Matsui, Kiyohide Fushimi, Naohiro Izawa, Tetsuro Yasui,
Yuho Kadono, Sakae Tanaka. Time trends and risk factors for perioperative complications in total ankle
arthroplasty: retrospective analysis using a national database in Japan. BMC Musculoskelet Disord.
2016.10; 17(1); 450― 142 ―

Public HealthPublic Health

37. Takashi Tagami, Hiroki Matsui, Masamune Kuno, Yuuta Moroe, Junya Kaneko, Kyoko Unemoto, Kiyohide Fushimi, Hideo Yasunaga. Early antibiotics administration during targeted temperature management after out-of-hospital cardiac arrest: a nationwide database study. BMC Anesthesiol. 2016.10; 16(1);89
38. Takashi Tagami, Hiroki Matsui, Chie Tanaka, Junya Kaneko, Masamune Kuno, Saori Ishinokami, Kyoko
Unemoto, Kiyohide Fushimi, Hideo Yasunaga. Amiodarone Compared with Lidocaine for Out-Of-Hospital
Cardiac Arrest with Refractory Ventricular Fibrillation on Hospital Arrival: a Nationwide Database Study.
Cardiovasc Drugs Ther. 2016.10; 30(5); 485-491
39. Toshiaki Isogai, Hiroki Matsui, Hiroyuki Tanaka, Kiyohide Fushimi, Hideo Yasunaga. Atrial natriuretic
peptide therapy and in-hospital mortality in acute myocardial infarction patients undergoing percutaneous
coronary intervention. Int. J. Cardiol.. 2016.11; 222; 163-170
40. Shotaro Aso, Hiroki Matsui, Kiyohide Fushimi, Hideo Yasunaga. The Eﬀect of Intraaortic Balloon Pumping Under Venoarterial Extracorporeal Membrane Oxygenation on Mortality of Cardiogenic Patients: An
Analysis Using a Nationwide Inpatient Database. Crit. Care Med.. 2016.11; 44(11); 1974-1979
41. Yasuo Nakahara, Hideo Yasunaga, Haruhi Inokuchi, Naoshi Ogata, Hiromasa Horiguchi, Shinya Matsuda,
Kiyohide Fushimi, Nobuhiko Haga. Mortality-Reducing Eﬀect of Rehabilitation for COPD: Observational
Propensity-Matched Cohort Study Using a Nationwide Database. Respir Care. 2016.11; 61(11); 14971504
42. Tsuyoshi Hamada, Hideo Yasunaga, Yousuke Nakai, Hiroyuki Isayama, Hiroki Matsui, Kiyohide Fushimi,
Kazuhiko Koike. No weekend eﬀect on outcomes of severe acute pancreatitis in Japan: data from thediagnosis procedure combination database. J. Gastroenterol.. 2016.11; 51(11); 1063-1072
43. Nobuaki Michihata, Hiroki Matsui, Kiyohide Fushimi, Hideo Yasunaga. Hospital volume and mortality
due to preterm patent ductus arteriosus. Pediatr Int. 2016.11; 58(11); 1171-1175
44. Maiko Yagi, Hideo Yasunaga, Hiroki Matsui, Kiyohide Fushimi, Masashi Fujimoto, Teruyuki Koyama,
Junko Fujitani. Eﬀect of early rehabilitation on activities of daily living in patients with aspiration
pneumonia. Geriatr Gerontol Int. 2016.11; 16(11); 1181-1187
45. Susumu Kunisawa, Kiyohide Fushimi, Yuichi Imanaka. Reducing Length of Hospital Stay Does Not
Increase Readmission Rates in Early-Stage Gastric, Colon, and Lung Cancer Surgical Cases in JapaneseAcute Care Hospitals. PLoS ONE. 2016.11; 11(11); e0166269
46. Ryuji Sasaki, Hideo Yasunaga, Hiroki Matsui, Nobuaki Michihata, Kiyohide Fushimi. Hospital Volume
and Mortality in Mechanically Ventilated Children: Analysis of a National Inpatient Database in Japan.
Pediatr Crit Care Med. 2016.11; 17(11); 1041-1044
47. Hironori Uematsu, Kazuto Yamashita, Susumu Kunisawa, Kiyohide Fushimi, Yuichi Imanaka. The economic burden of methicillin-resistant Staphylococcus aureus in community-onset pneumonia inpatients.
Am J Infect Control. 2016.12; 44(12); 1628-1633
48. Ryota Niikura, Hideo Yasunaga, Atsuo Yamada, Hiroki Matsui, Kiyohide Fushimi, Yoshihiro Hirata,
Kazuhiko Koike. Factors predicting adverse events associated with therapeutic colonoscopy for colorectal
neoplasia: a retrospective nationwide study in Japan. Gastrointest. Endosc.. 2016.12; 84(6); 971-982.e6
49. Takashi Tagami, Hiroki Matsui, Saori Ishinokami, Masao Oyanagi, Akiko Kitahashi, Reo Fukuda, Kyoko
Unemoto, Kiyohide Fushimi, Hideo Yasunaga. Amiodarone or nifekalant upon hospital arrival for refractory ventricular ﬁbrillation after out-of-hospital cardiac arrest. Resuscitation. 2016.12; 109; 127-132
50. Tomoki Wada, Hideo Yasunaga, Hiromasa Horiguchi, Kiyohide Fushimi, Takehiro Matsubara, Susumu
Nakajima, Naoki Yahagi. Ozagrel for Patients With Noncardioembolic Ischemic Stroke: A Propensity
Score-Matched Analysis. J Stroke Cerebrovasc Dis. 2016.12; 25(12); 2828-2837― 143 ―

Public HealthPublic Health[Conference Activities & Talks]
1. Kiyoshi Murata，Yasuhiro otomo，Kiyohide Fushimi．. Quality Management of Acute Care Surgery
Training Program based on PDCA cycle. 17th European Congress of Trauma and Emergency Surgery
2016.04.24 Vienna, Austria
2. Ohta, M., Fushimi, K. . Real-World Comparing Intravenous Busulfan to Total Body Irradiation in
Hematopoietic Stem Cell Transplantation with A Japanese Diagnosis Procedure Combination(DPC)
Database. ISPOR 7th Asia-Paciﬁc Conference 2016.09.03 Singapore
3. Mutsuko Moriwaki，Yuko Sase，Mikayo Toba，Kiyohide Fushimi．. Visualization of the current situation of the use of antibacterial drugs by using medical data and an approach to improvement activities.
International Forum on Quality and Safety in Healthcare. 2016.09.26 Singapore
4. Mikayo Toba, Mutsuko Moriwaki, Noriko Oshima, Kimio Wakana, Akira Wakabayashi, Satoshi Kobayashi,
Kiyohide Fushimi.. Caluculation of inpatient mortality after surgeryfor uterine endometrial cancer using a anationwide administractive database. International Forum on Quality and Safety in Healthcare.
2016.09.26 Singapore― 144 ―

Public Health
HealthPublicLife Sciences and Bioethics

Masayuki Yoshida
Yusuke Ebana
Hiroko Kohbata
Mizuko Osaka
Eiichiro Kanda(1)

OutlineDepartment of Life Sciences and Bioethics （Bioethics Research Center) oﬀers classes and seminars regarding
bioethics, research ethics, and clinical ethics in Graduate School of Medical and Dental Sciences, Graduate Schoolof Health Care Sciences, and School of Medicine. Our lecture includes fundamental bioethics and research ethics
so that students can absorb the current concept of the bioethics and research ethics. We try to include clinicalmaterials such as cases of genetic counseling, where ethics-based approach is critically important.
Apart from class for juniors, we give bioethics seminars for hospital staﬀ and faculties based on the research
ethics guideline revised 2008, in which attendance of bioethics lecture is mandatory for any person who conducts
medical research.
We dynamically participated in extra-campus activities; such as the ethical committee members of the National
Institute of Health etc.

(2)

Publications[Original Articles]
1. Yuki Hasegawa, Satomi Hamada, Takuro Nishimura, Takeshi Sasaki, Yusuke Ebana, Mihoko Kawabata,
Masahiko Goya, Mitsuaki Isobe, Takatoshi Koyama, Tetsushi Furukawa, Kenzo Hirao, Tetsuo Sasano.
Novel Dielectric Coagulometer Identiﬁes Hypercoagulability in Patients with a High CHADS2 Score without Atrial Fibrillation. PLoS ONE. 2016; 11(6); e0156557
2. Yusuke Ebana, Hiroko Terui-Kohbata, Sayako Takahashi, Yoshida Masayuki.. Genetic counseling fora case with breast cancer who underwent a whole-exome sequencing in a private company The 13th
International Congress of Human Genetics Program. 2016; 229
3. Eiichiro Kanda, Masumi Ai, Mitsuyo Okazaki, Masayuki Yoshida, Yoshitaka Maeda. Association of HighDensity Lipoprotein Subclasses with Chronic Kidney Disease Progression, Atherosclerosis, and Klotho.
PLoS ONE. 2016; 11(11); e0166459
4. Kataoka H, Ariyama Y, Deushi M, Osaka M, Nitta K, Yoshida M.. Inhibitory Eﬀect of Serotonin Antagonist on Leukocyte-Endothelial Interactions In Vivo and In Vitro. PROS ONE. 2016.01; 11(1); e0147929
5. Ito S, Osaka M, Edamatsu T, Itoh Y, Yoshida M.. Crucial Role of the Aryl Hydrocarbon Receptor (AhR)
in Indoxyl Sulfate-Induced Vascular Inﬂammation. Journal of Atherosclerosis Thrombosis. 2016.02;
6. Mizuko Osaka, Shunsuke Ito, Masaki Honda, Yukihiro Inomata, Kensuke Egashira, Masayuki Yoshida.
Critical role of the C5a-activated neutrophils in high-fat diet-induced vascular inﬂammation. Scientiﬁc
Reports. 2016.02; 6; 21391― 145 ―

Public HealthPublicHealth

7. Yoichi Otaki, Yusuke Ebana, Shunji Yoshikawa, Mitsuaki Isobe. Dielectric permittivity change detects
the process of blood coagulation: Comparative study of dielectric coagulometry with rotational thromboelastometry. Thromb. Res.. 2016.06; 145; 3-11
8. Hirokazu Ohigashi, Natsuko Tamura, Yusuke Ebana, Masayoshi Harigai, Yasuhiro Maejima, Takashi
Ashikaga, Mitsuaki Isobe. Eﬀects of immunosuppressive and biological agents on refractory Takayasu
arteritis patients unresponsive to glucocorticoid treatment. J Cardiol. 2016.08;
9. Shunsuke Ito, Mizuko Osaka, Takeo Edamatsu, Yoshiharu Itoh, Masayuki Yoshida. Crucial Role of the
Aryl Hydrocarbon Receptor (AhR) in Indoxyl Sulfate-Induced Vascular Inﬂammation. J. Atheroscler.
Thromb.. 2016.08; 23(8); 960-975
10. Michiyo Deushi, Mizuko Osaka, Kaku Nakano, Kyoichi Osada, Kensuke Egashira, Masayuki Yoshida.
Ezetimibe reduced hepatic steatosis induced by dietary oxysterols in nonhuman primates. FEBS Open
Bio. 2016.10; 6(10); 1008-1015
11. Tani M, Horvath KV, Lamarche B, Couture P, Burnett JR, Schaefer EJ, Asztalos BF. Eﬀects of Lipoprotein Lipase and Hepatic Lipase Deﬁciency on High-Density Lipoproteins. Atherosclerosis . 2016.10; 253;
7-14
12. Lian Liu, Yusuke Ebana, Jun-Ichi Nitta, Yoshihide Takahashi, Shinsuke Miyazaki, Toshihiro Tanaka,
Masatoshi Komura, Mitsuaki Isobe, Tetsushi Furukawa. Genetic Variants Associated With Susceptibility
to Atrial Fibrillation in a Japanese Population. Can J Cardiol. 2016.11;[Misc]
1. Yusuke Ebana. Ethics in Clinical Research Obstet. Gynecol.. 2016.10; 83(10); 1119-1123[Conference Activities & Talks]
1. Osaka Mizuko, Ito Shunsuke, Yoshida Masayuki. High-Fat Diet-triggered Neutrophil Activation Initiates
Leukocyte Adhesion to Murine Femoral Artery in vivo. Japanese Circulation Society Scientiﬁc Meeting2016.03
2. Yusuke Ebana, Hiroko Terui-Kohbata, Sayako Takahashi, Yoshida Masayuki. Genetic counseling for a casewith breast cancer who underwent a whole-exome sequencing in a private company. The 13th International
Congress of Human Genetics 2016.04
3. Yusuke Ebana Hiroko Kohbata, Sayako Takahashi, Masayuki Yoshida. Genetic counselling for a case withbreast cancer who underwent a whole-exome sequencing in a private company. The 13th International
Congress Society of Human Genetics 2016.04.06
4. Hiroko Kohbata, Yusuke Ebana, Sayako Takahashi, Masayuki Yoshida. Genetic Counseling for the Resultof a Company’s Personal Exome Sequencing: a Case Report. Japanese Society for Genetic Counseling2016.04.08 Kyoto
5. Osaka M, Yoshida M. Role of complement component C5a for development of atherosclerosis.. The 48th
Annual Scientiﬁc Meeting of the Japan Atherosclerosis Society 2016.07.14 Tokyo
6. Mizuko Osaka, Masaki Honda, Yukihiro Inomata, Kensuke Egashira, Masayuki Yoshida. C5a play an important role in high fat diet-induced neutrophil activation. XXVIth INTERNATIONAL COMPLEMENTWORKSHOP 2016.09.04
7. Mariko Tani, Yuko Kamata, Michiyo Deushi, Mizuko Osaka, Masayuki Yoshida. 7-ketocholesterol Induces
Leukocyte Interaction to Endothelial Cells thorough p38MAPK-dependent pathway. 19th International
Vascular Biology Meeting 2016.10 Sheraton Boston Hotel, Boston, USA
8. Mizuko Osaka, Masaki Honda, Yukihiro Inomata, Masayuki Yoshida. Critical role of complement C5aand neutrophils during high-fat diet-induced vascular inﬂammation.. 19th International vascular biology
meeting 2016.10.30 Boston
9. Osaka M, Yoshida M. High fat diet induces vascular inﬂammation via upregulation of complement component C5a.. the Society of Cerebral-Cardio-Vascular Anti-Aging 2016.12.17 Tokyo― 146 ―

Public Health
HealthPublicForensic Dentistry

Professor Koichi SAKURADA
Assistant Professor Hajime UTSUNO
Assistant Professor Namiko ISHII
Graduate Student Saki MINEGISHI(1)

OutlineForensic dentistry plays an important role in society through the use identiﬁcation of victims after major
accidents or disasters using dental ﬁndings, as well as the identiﬁcation of cadavers or persons from biologicalsamples in relation to crime. In particular, the establishment of two laws related to cause of death investigationin June 2012 further promoted research, identiﬁcation, and education related to individual identiﬁcation. The
primary function of our laboratory is the identiﬁcation of individuals from hard tissues such as teeth and bones,
soft tissues, body ﬂuids, or facial images, using the latest molecular biological and imaging techniques.
(2)

Research

1. Individual identiﬁcation● Identiﬁcation based on dental ﬁndings● Identiﬁcation using hard tissues such as teeth and bones, soft tissues, and body ﬂuids● Identiﬁcation based on facial reconstruction and image analysis
2. Child abuse and neglect
3. Dental accidents and lawsuits
4. Problems associated with the dental care system
5. Forensic toxicology(3)

Education

We teach dental students a relation between death investigation systems and dentists and make them understandthat society expects them as dentists to perform individual identiﬁcation based on dental ﬁndings. Also, students
are likely to have opportunities to assist the regional administrative and police activities in the future. To
protect the rights of the deceased individuals and improve public health, dental students need to acquire basic
knowledge about forensic medical sciences including postmortem changes and cause of death identiﬁcation. To
foster independent researchers, we teach graduate students the latest research directions in forensic dentistry andhow to plan their own research project. In addition, students learn practical individual identiﬁcation methods
and their importance through forensic autopsy.
(4)

Lectures & Courses

We believe that students learn more eﬀectively in an environment where they can simultaneously conduct
practical work and research.

― 147 ―

Public HealthPublicHealth(5)

Clinical Services & Other Works

Forensic autopsy for the identiﬁcation of cadavers and other related activities. Individual identiﬁcation following
accidents or disasters. Participation in disaster prevention and individual identiﬁcation training programs heldby various communities.
(6)

Publications[Original Articles]
1. Ken Watanabe, Tomoko Akutsu, Koichi Sakurada. Development of a Real-Time PCR-Based Method
for Analyzing Semen-Speciﬁc Unmethylated DNA Regions and Methylation Status in Aged Body Fluid
Stains. J. Forensic Sci.. 2016.01; 61 Suppl 1; S208-S212
2. Suguru Torimitsu, Yohsuke Makino, Hisako Saitoh, Ayaka Sakuma, Namiko Ishii, Daisuke Yajima, Go
Inokuchi, Ayumi Motomura, Fumiko Chiba, Rutsuko Yamaguchi, Mari Hashimoto, Yumi Hoshioka, Hirotaro Iwase. Stature estimation from skull measurements using multidetector computed tomographic
images: A Japanese forensic sample. Leg Med (Tokyo). 2016.01; 18; 75-80
3. Ken Watanabe, Tomoko Akutsu, Ayari Takamura, Koichi Sakurada. Evaluation of a blood-speciﬁc DNA
methylated region and trial for allele-speciﬁc blood identiﬁcation from mixed body ﬂuid DNA. Leg Med(Tokyo). 2016.09; 22; 49-53
4. Namiko Ishii, Ayaka Sakuma, Yohsuke Makino, Suguru Torimitsu, Daisuke Yajima, Go Inokuchi, Ayumi
Motomura, Fumiko Chiba, Yumi Hoshioka, Hirotaro Iwase, Hisako Saitoh. Incidence of three-rooted
mandibular ﬁrst molars among contemporary Japanese individuals determined using multidetector computed tomography. Leg Med (Tokyo). 2016.09; 22; 9-12
5. Suguru Torimitsu, Yohsuke Makino, Hisako Saitoh, Ayaka Sakuma, Namiko Ishii, Daisuke Yajima, Go
Inokuchi, Ayumi Motomura, Fumiko Chiba, Rutsuko Yamaguchi, Mari Hashimoto, Yumi Hoshioka, Hirotaro Iwase. Sexual determination based on multidetector computed tomographic measurements of thesecond cervical vertebra in a contemporary Japanese population. Forensic Sci. Int.. 2016.09; 266; 588.e1588.e6
6. N Ishii, Y Makino, M Fujita, A Sakuma, S Torimitsu, F Chiba, D Yajima, G Inokuchi, A Motomura,N H Iwase, H Saitoh. Assessing age-related change in Japanese mental foramen opening direction using
multidetector computed tomography. J Forensic Odontostomatol. 2016.12; 2(34); 11-20[Books etc]
1. Koichi Sakurada. Teeth speak louder than words the great mission that a dentist can contribute to thesociety . DENTAL DIAMOND, 2016.07
2. Koichi Sakurada. Teeth speak louder than words what methods are used for private identiﬁcation ? .
DENTAL DIAMOND, 2016.08
3. Koichi Sakurada. Teeth speak louder than words why is the tooth useful for private identiﬁcatoin? .
DENTAL DIAMOND, 2016.09
4. Koichi Sakurada. Teeth speak louder than words private identiﬁcation by forensic autopsies . DENTAL
DIAMOND, 2016.10
5. Koichi Sakurada. Teeth speak louder than words oral photograph and dental chart . DENTAL DIAMOND, 2016.11
6. Koichi Sakurada. Teeth speak louder than words dental X-rays and CT images . DENTAL DIAMOND,
2016.12[Misc]
1. Victor A Voicu, Andrei Valentin Medvedovici, Koichi Sakurada, Hikoto Ohta, Flavian Stefan Rǎdulescu,
Dalia Simona Miron. The forgotten or underestimated relevance of biopharmaceutical-based assessments
for the oral absorption studies of oxime reactivators. Expert Opin Drug Metab Toxicol. 2016.07; 12(7);743-752― 148 ―

Public Health
HealthPublic[Conference Activities & Talks]
1. Susumu Ohtani, Ran Iguchi, Hidenori Nene, Noboru Adachi, Kanako Noritake, Joji Funakoshi, Koichi
Sakurada, Koichi Uemura, Yoshihiro Yamada. A case that the age estimation from the tooth by theamino acid racemization method was particulary eﬀective for identiﬁcation . The 100 Congress of theJapanese Society of Legal Medicine 2016.06.17 Shinagawa-ku, Kyurian
2. Torimitsu S, Makino Y, Saitoh H, Sakuma A, Ishii N, Yajima D, Inokuchi G, Motomura A, Chiba F,
Yamaguchi R, Hashimoto M, Hoshioka Y, Iwase H.. Stature estimation from skull measurements using
multidetector computed tomographic images: A Japanese forensic sample.. The 100st Congress Of The
Japanese Society Of Legal Mrdicine 2016.06.17 Kyuriann,Higashiooi, Shinagawa-ku, Tokyo
3. Saitoh H, Yusa K, Moriya T, Takeyama M, Sakuma A, Ishii N, Abe H, Takahashi S, Chiba F, Torimitsu
S, Iwase H, Iino M, Tokanai H.. The radiocarbon birth-dating estimation using teeth : a study ofsample processing. The 100th Congress Of The Japanese Society Of Legal Medicine 2016.06.17 Kyuriann,
Higashiooi, Shinagawa-ku, Tokyo
4. Hajime Utsuno, Namiko Ishii, Saki Minegishi, Koichi Sakurada. Oculusion state of the gender gap whichshould be noted when performing the face restruction method. The 15th National Meeting of Police
Dentist Society 2016.09.03 Gifu city
5. Hajime Utsuno, Namiko Ishii, Saki Minegishi, and Koichi Sakurada.. Signiﬁcant diﬀerence of occlusion
and Sexual demorphisms for then performing facial reconstruction.. The 15th National Meeting of Police
Dentist Society 2016.09.03 Gifu
6. Saegusa F, Saitoh H, Ishii N, Sakuma A, Hiraga T, Takahashi T, Nakayama S, Hayashi K, Omori M,
Uchida K, Shishikura K, Kimata S, Saito H.. For the dental identiﬁcation , the Japan dental associationin cooperation with the police work with the department of Legal Medicine at Chiba University . The
15th National Meeting of Police Dentist Society 2016.09.03 Gifu Grand Hotel, Gifu-shi
7. Hajime Utsuno, Namiko Ishii, Saki Minegishi, Koichi Sakurada. Oculusion state of the gender gap whichshould be noted when performing the face restruction method. The 15th National Meeting of Police
Dentist Society 2016.09.03 Gifu city
8. Hajime Utsuno. Archaeological Facial Reconstruction from Juvenile Skull. The 70th Annual Meeting of
the Anthropological Society of Nippon 2016.10.10 Niigata
9. Koichi Sakurada, Ken Watanabe, Tomoko Akutsu. Seminar 4 :Introduction of forensic scientiﬁc methods
from bloodstains and body ﬂuid stains. 10th Anniversary Meeting of Japanese Society of Forensic Dental
Science 2016.11.06 Station Conference Tokyo
10. Torimitsu S, Makino Y, Saitoh H, Sakuma A, Ishii N, Yajima D, Inokuchi G, Motomura A, Chiba F,
Yamaguchi R, Hashimoto M, Hoshioka Y, Iwase H.. Sex estimation based on scapula analysis in a
Japanese population using multidetector computed tomography.. The 10th Congress Of The JapaneseSociety Of Forensic Dental Science 2016.11.06 Station Conference Tokyo
11. Sugimoto T., Utsuno H.. Questionnair survey on relaps of post orthodontic treatment in the patients ofOmiya Orthodontic Clinin. The 75th Annual Meeting of the Japanese Orthodontic Society 2016.11.08
Tokushima
12. Koichi Sakurada. Project for Promoting Leading-edge Research in Oral Science, Kickoﬀ Symposium, Session 2:Society and Education. The 81st Annual Meeting of the Stomatological Society of Japan 2016.11.26
Tokyo Medical and Dental University[Social Contribution]
1. Individual identiﬁcation using dental ﬁndings (50 cases), 2016, 2016.01.01 - 2016.12.31― 149 ―

Public HealthPublic Health

Health Care Economics

Koichi Kawabuchi
Isao Igarashi(1)

Outline

The role of health care extends to improving such aspects of life as dietary habit and relationship with others,
and is deeply connected to quality of life. Looking back, however, discussion on healthcare has too often
originated from political dynamics and interests of parties involved, and not from the voices of general public.
This is due partially to the lack of quality data available among the people. The reality of healthcare andwhat it brings to the society are not necessarily always clear to the general public. In terms of dental care
especially, it is hard to say that enough evidences have been established and widely recognized among people
to the extent that matches to its importance in providing quality life. Thus, we apply economics in conducting
interdisciplinary review of healthcare along with other related ﬁelds, and pursue how the healthcare systemshould be for the people.

(2)

ResearchResearch activities involve conducting analysis on phenomena and observations in health care from the viewpoint of macro as well as micro economics.
Main focuses are:
1) Cross-sectional research on healthcare, dental care, nursing care, long-term care, and pharmaceutics fromthe viewpoint of economics
2) Proposals on policy making in eﬃcient delivery of healthcare, nursing care, and long-term care3) Borderless and mutual development of various specialties such as dental care, healthcare, economics, management and accounting.

(3)

Education

Understanding the methods of research on phenomena and observations in health care ﬁeld through economics
point of view. As we have many part-time students with jobs as well as foreign students, the lecture will
be centered around such topics as the approach to a research theme in economics and other social sciences(especially empirical studies) , how to proceed with the research, and paper writing. Speciﬁcally, we will
provide outline of healthcare economics by a weekly lecture for Ph. D. candidates as well as once a year lecture
for the master course. Some of them adapt more interactive style of problem solving with input from visiting
lecturers. Emphasis is placed on methods of quantitative analysis, learning both theoretical and empiricalapproaches to phenomena and observations in health care ﬁeld through economics point of view. Lectures forundergraduate education will be focused on the outline of healthcare economics in dental care, with speciﬁcthemes as 1) Economical analysis of dental healthcare, 2) Expenditure on dental care, 3) Reimbursement, and
4) Quality assessment of dental care.

― 150 ―

Public HealthPublic Health(4)

Lectures & Courses

Faced with recent changes in healthcare and long-term care, core hospitals and other healthcare related institutions in communities with responsibility of supporting front-line healthcare long for personnel competent inhealthcare management. Call for such personnel is strong among research organizations and public oﬃces as
well, looking for those who are proﬁcient in qualitative and quantitative analysis. Therefore, we aim to train
students to be capable in making immediate contribution to the healthcare and welfare ﬁeld, and to educate
future "academic doctors" who can voice their messages in policy making.
(5)

Clinical Services & Other Works

Igarashi, Assistant Professor in our department, practices three times a week in the clinic for Oral Diagnosis
and General Dentistry of the Dental Hospital. Findings from our research activities are shared to the publicthrough papers, reports, lectures and symposiums in both academic and less academic settings. Comments on
healthcare reform, for instance, have been televised on and printed in various media.
(6)

Publications[Misc]
1. Kazumitsu Nawata, Koichi kawabuchi. Comparison of the Length of Stay and Medical Expenditures
among Japanese Hospitals for Type 2 Diabetes Treatments: The Box-Cox Transformation Model under
Heteroscedasticity Health. 2016.01; 8(1);2. Koichi Kawabuchi, Yusuke Kabeya. FY2016 Estimating the Impact of the New Income Deduction System
for Over-the-Counter Drug Expenses- JAPAN HOSPITALS. 2016.07; 35;[Conference Activities & Talks]
1. Policies on Healthcare and Nursing care in Japan and The Integrated Community Care System.. International (Sino-Japan) Summit on Integrated Care 2016.04.09
2. Koichi Kawabuchi. Looking for solutions to issues in elderly care. CHINA-HOSPEQ 2016 2016.08.19
3. Koichi Kawabuchi, Yusuke Kabeya. Impact of promoting self-medication on medical costs in Japan: a
new research proposal. 11th WSMI Asia-Paciﬁc Conference & 3rd APSMI General Assembly Meeting at
Nagoya 2016.10.13
4. Koichi Kawabuchi. Promoting self-medication in Japan∼focusing on OTC drugs and tax cut policy. the26th International Symposium at the Center for Japanese Studies, Fudan University 2016.11.05― 151 ―

Public HealthPublic Health

Dental Education Development

Professor: Ikuko MORIO
Assistant: Professor Naoko SEKI
Graduate Student: Chinatsu MATSUKAWA
Graduate Student: Akira TAKINAGA
Graduate Student: NGUYEN THI THANH TAM
Graduate Student: Mio NAITO(1)

Research

1) Research on curriculum for health care professional education
2) Comparative study of domestic and international dental education
3) Research and development of educational methods in health care professional education
4) Research and development of English education programs in health care professional education(2)

Education

Main educational goal of this section as part of graduate school is to help students in health care sciences learn thebasics of medical/dental curriculum: educational objectives, strategies and evaluation. This section is currently
involved in the undergraduate dental education as the coordinators of multiple modules: the students research
project, courses for global communication, and the electives including various English courses and courses forinternational exchange for dental students.
(3)

Clinical Services & Other Works[ Coordination for Seminar, Symposium, Workshop or other events]
1.Essential Expertise for Clinical Dentistry 1 (Seminar), Tokyo, Japan (TMDU). February 26, March 25, April
15, May 20, June 17, July 15.
2.Essential Expertise for Clinical Dentistry 2 (Seminar), Tokyo, Japan (TMDU). October 27, November 24,
December 15.
3. TMDU Dental Training Program 2016, Tokyo, Japan. October 7-18.
(4)Publications[Original Articles]
1. Takehara S, Wright FAC, Kawaguchi Y, Ishida Y, Morio I, and Tagami J. Characteristics of undergraduate
dental students in Japan: English competency and willingness to study abroad International Dental
Journal. 2016; 66; 311-317
2. Seki N, Moross J, Sunaga M, Hobo K, Miyoshi T, Nitta H, Kinoshita A, Morio I. Evaluation of simulation
learning materials use to ﬁll the gap in Japanese dental English education. Journal of Medical and Dental
Sciences. 2016.03; 63(1); 1-8
3. Rena Oguro, Masatoshi Nakajima, Naoko Seki, Alireza Sadr, Junji Tagami, Yasunori Sumi. The role ofenamel thickness and refractive index on human tooth colour. J Dent. 2016.08; 51; 36-44― 152 ―

Public HealthPublic Health

4. Asami Aida, Masatoshi Nakajima, Naoko Seki, Yukinori Kano, Richard M Foxton, Junji Tagami. Eﬀect
of enamel margin conﬁguration on color change of resin composite restoration. Dent Mater J. 2016.08;
35(4); 675-683[Conference Activities & Talks]
1. Naito M,Shinada K,Kunitsuka K,Yamamoto R,Onishi T,Nishi M,Seki N,Morio I,Taniyama K. Intervention
survey on oral health at newspaper printing factories. 89th Annual Meeting of the Japan Society for
Occupational Health 2016.05.27 Fukushima city
2. Morio I. Quality Assurance System in Japanese Dental Education. The 12th International Conference of
Asian Academy of Preventive Dentistry 2016.05.29 Tokyo, Japan
3. Kurumi IDE, Masatoshi NAKAJIMA, Keiichi HOSAKA, Masahiro TAKAHASHI, Naoko SEKI, Junji
TAGAMI. Eﬀect of cavity depth on Ultimate Tensile Strength of bulk ﬁll ﬂowable resin. The 144th
Meeting of the Japanese Society of Conservative Dentistry 2016.06.10 Tochigi, Japan
4. Morio I. Dental Education and Accreditation in Japan. The 1st East Asian Conference on Dental Education and Accreditation 2016.06.25 Seoul, Republic of Korea
5. Morio I. Globalization of universities - current situation and future direction. Annual Meeting of the 35th
Japanese Dental Education Association 2016.07.02 Osaka, Japan
6. Nguyen T, Seki N , Morio I. Comparing stress of dental students in clinical/preclinical practices at the
University of Ho Chi Minh City, Vietnam. Annual Meeting of the 35th Japanese Dental Education
Association 2016.07.02 Osaka, Japan
7. Moross J, Seki N, Sunaga M, Otsuka H, Naito M, Shinada K, Morio I, Kinoshita A. Development of ESL
clinical simulation teaching materials for dental hygienist education. Annual Meeting of the 35th Japanese
Dental Education Association 2016.07.02 Osaka, Japan
8. Nguyen T, Seki N, Morio I. Association between stress and academic performance in dental students. The
27th annual scientiﬁc meeting, South East Asia Association for Dental Education. 2016.09.09 Ho Chi
Minh City, Vietnam
9. Tsuruta J, Noritake K, Mizutani K, Seki N, Kondo K, Arakawa S, Araki K. A new IPW course -working
in a dental team. The 27th annual scientiﬁc meeting, South East Asia Association for Dental Education.
2016.09.09 Ho Chi Minh City, Vietnam
10. Morio I, Araki K. Quality Assurance of Dental Education in Japan. 27th Annual Scientiﬁc MeetingSouth
East Asia Association for Dental Education (SEAADE) 2016.09.09 Ho Chi Minh City, Vietnam
11. Morio I. What has been brought about by the implementation of this 5-year project. Re-Inventing Japan
Project_Inter-university exchange program toward medical and dental networking in Southeast Asia International Symposium "Developing Global Health Leaders" 2016.10.11 Tokyo, Japan
12. Anna Miyayasu, Manabu Kanazawa, Yuriko Komagamine, Naoko Seki. Decision making for prosthodontics treatment in a case of multiple tooth loss. Essential Expertise for Clinical Dentistry 2 2016.10.27
Tokyo, Japan
13. Seki N, Moross J, Otsuka H, Sunaga M, Naito M, Takatsuna Y, Kondo K, Shinada K, Morio I, Kinoshita
A. Learning outcome provided by ESL clinical simulation teaching materials for dental hygiene education.
81st Annual Meeting of the Stomatological Society 2016.11.26 Tokyo
14. Takinaga A, Seki N, Morio I. Present Situation after Employing the Foreign Nurses Based on the Economic
Partnership Agreement (EPA nurses) -From the Experience at Japanese Red Cross Ashikaga Hospital-.
81st Annual Meeting of the Stomatological Society 2016.11.26 Tokyo
15. Nguyen T, Seki N, Morio I . Stress and academic performance in dental students at Tokyo Medical and
Dental University. 81st Annual Meeting of the Stomatological Society 2016.11.26 Tokyo― 153 ―

Public HealthPublic Health

Oral Health Promotion

Professor Yoko Kawaguchi
Associate Professor Masayuki Ueno
Assistant Professor Takashi Zaitsu
Hospital Staﬀ Akiko OhshiroOﬃce administrator Reika Nagaoka
Research Assistant Professor Sachiko Takehara (the Institute of Global Aﬀairs)
Registered Resident Hiromi Nishiyama
Graduate Student Anastasiya Blizniuk(∼September)
Nguyen Thi Hoang Yen
Yuka Shizuma
Kaung Myat Thwin
Toshiya Kanazawa
Takashi Tanemura
Jin Aoki
Mitsue Kamisawa(1)

ResearchResearch Subjects
Research topics are innovative, academic and international research in the ﬁeld of dental public health andpreventive dentistry to proceed with oral health promotion that contributes to human health. The current
main research themes are:
1. Epidemiology and prevention of dental disease
2. Oral health care system
3. Relationship between oral health and general health
4. Oral health promotion
5. Diagnosis and treatment system construction of oral malodor
6. International oral health(2)

Lectures & Courses

1) Graduate School, Oral Health Promotion
The educational purpose is to foster professionals in dental public health and preventive dentistry who can thinkoral health problems as related issues with living environment, life style, health policy and social condition, andcan conduct innovative, academic and international research on oral health for maintaining and improving oral
health.
2) Undergraduate Education
The department is in charge of module units of Introduction to dentistry , Environment and society II
and Comprehensive problem exercise for the third year dental students, and module units of Basis for
dentistry , Prevention and health management I , Prevention and health management II and Dentistry
and nutrition" for the fourth year dental students. The department is also in charge of Experiential research― 154 ―

Public HealthPublic Healthexercise for the fourth year dental students, and Comprehensive clinical practice phase I & II
and ﬁfth year dental students, in cooperation with other departments.

for the fourth(3)

Clinical Services & Other Works

Clinical Services
Fresh breath clinic in Dental hospital, Tokyo Medical and Dental University is a special clinic for diagnosis,
treatment and prevention of oral malodor. About half of oral malodor patients are referred from other departments in the dental hospital or outside dental clinics. Other patients visit the clinic by ﬁnding the informationof the clinic from mass media such as the internet, newspapers and television.
For oral malodor examination, gas chromatography and gas sensor instrument are used to measure the concentration of volatile sulfur compounds (VSCs) along with the organoleptic test. Oral malodor is treated basedon diagnosis by precise measurement and oral examination, besides psychological aspects of the patient arepaid attention. Treatment of oral malodor needs continuous periodontal disease management and oral care incooperation with oral care department in the dental hospital and patient s family dentist.

(4)

Publications[Original Articles]
1. S Takehara,FAC Wright,Y Kawaguchi,Y Ishida,I Morio,J Tagami. Characteristics of undergraduate dental
students in Japan:English competency and willingness to study abroad International Dental Journal. 2016;
66; 311-317
2. Kaung Myat Thwin, Takashi Zaitsu, Masayuki Ueno, Yoko Kawaguchi.. Early Childhood Caries andRelated Risk Factors among Myanmar Preschool Children Int J Clin Prev Dent. 2016; 12(4); 229-235
3. Masayuki Ueno, Norie Sawada, Manami Inoue, Shoichiro Tsugane, Yoko Kawaguchi.. Association between
adult s height and dentition status: JPHC oral health study Asian Pac J Health Sci. 2016; 3(4s); 81-88
4. Sachiko Komori, Takashi Zaitsu, Sachiko Takehara, Masayuki Ueno, Yoko Kawaguchi. The relationshipof salivary viscosity with halitosis Int J Clin Prev Dent. 2016; 12(1); 13-22
5. H Rani,Ueno M,Zaitsu T,Kawaguchi Y. Oral malodour among adolescents and its association with healthbehaviour and oral health status International Journal of Dental Hygiene. 2016; 14; 135-141
6. Blizniuk A, Ueno M, Zaitsu T, Kawaguchi Y. Association between self-reported and clinical oral healthstatus in Belarusian adults. Journal of Investigative and Clinical Dentistry . 2016.01; 0(0); 1-6
7. Blizniuk A, Furukawa S,Ueno M, Kawaguchi Y. Evaluation of English Websites on Dental Caries by Using
Consumer Evaluation Tools 2016.01; 0(0); 1-7
8. Tumurkhuu T, Fujiwara T, Komazaki Y, Kawaguchi Y, Tanaka T, Inazawa J, Ganburged G, Bazar A,
Ogawa T, Moriyama K. Association between maternal education and malocclusion in Mongolian adolescents: a cross-sectional study. BMJ Open. 2016.11; 6(11);[Books etc]
1. Yoko Kawaguchi, Sachiko Takehara, Yuji Ishida, Kazuhiro Yonemoto. Dental Terminology in 8 Languages.
Oral Health Association of Japan, 2016.05[Conference Activities & Talks]
1. Yoko Kawaguchi. Strategies and approaches for tackling Early Childhood Caries(ECC). The36th Myanmar Dental Conference 2016.01.26 University of dental Medicine Yangon、Myanmar― 155 ―

Public HealthPublic Health

2. Sachiko Takeheara,Ikuko Morio,Yoko Kawaguchi. Eﬀects of exchange programs on perception among
Japanese/overseas dental students. The 12th International Conference of Asian Academy of PreventiveDentistry. Joint meeting with The 65th General Meeting of Japanese Society for Oral Health 2016.05.27
Tokyo Medical and Dental University
3. Mitsue Kamisawa,Mitsue Onodera,Takashi Zaitsu,Masayuki Ueno,Yoko Kawaguchi,. Oral health care forpsychiatric inpatients by nurses. The 12th International Conference of Asian Academy of PreventiveDentistry. Joint meeting with The 65th General Meeting of Japanese Society for Oral Health 2016.05.27
Tokyo Medical and Dental University
4. Akiko Oshiro,Yuka Shizuma,Toshiya Kamisawa,Mitsue Kamisawa,Sachiko Takehara,Takashi Zaitsu,Masayuki
Ueno,Yoko Kawaguchi. Relationship between oral malodor and the number of bacteria in tongue coating,.
The 12th International Conference of Asian Academy of PreventiveDentistry. Joint meeting with The 65th
General Meeting of Japanese Society for Oral Health 2016.05.27 Tokyo Medical and Dental University
5. Masayuki Ueno,Satoko Ohara,Yoko Kawaguchi. The association of active and passive smoking with oral
health in adults, The 12th International Conference of Asian Academy of PreventiveDentistry. The 12th
International Conference of Asian Academy of PreventiveDentistry. Joint meeting with The 65th General
Meeting of Japanese Society for Oral Health 2016.05.27 Tokyo Medical and Dental University
6. Takashi Zaitsu,Toshiya Kanazawa,Kaung Myat Thwin,Thi Hoang Yen Nguyen,Akiko Oshiro, Masayuki
Ueno,Yoko Kawaguchi. Oral health behaviors and symptoms of employees classiﬁed by worksite industry.
The 12th International Conference of Asian Academy of PreventiveDentistry. Joint meeting with The 65th
General Meeting of Japanese Society for Oral Health 2016.05.27 Tokyo Medical and Dental University
7. Takashi Zaitsu,Mitsue Kamisawa,Yuka Shizuma,Anastasiya Blizniuk,Sachiko Takehara,Reika Nagaoka,Masayuki
Ueno,Yoko Kawaguchi. Oral health status of employees classiﬁed by worksite industry. The 12th International Conference of Asian Academy of PreventiveDentistry. Joint meeting with The 65th General
Meeting of Japanese Society for Oral Health 2016.05.27 Tokyo Medical and Dental University
8. Yuka Shizuma ,Takashi Zaitsu, Masayuki Ueno ,Yoko Kawaguchi. Association of self-assessment with oral
health status and behavior among adolescents. The 12th International Conference of Asian Academy ofPreventiveDentistry. Joint meeting with The 65th General Meeting of Japanese Society for Oral Health2016.05.27 Tokyo Medical and Dental University
9. Haslina Rani, Masayuki Ueno, Takashi Zaitsu, Yoko Kawaguchi. A case report on volatile sulﬁde compounds changes in breath. The 12th International Conference of Asian Academy of PreventiveDentistry.
Joint meeting with The 65th General Meeting of Japanese Society for Oral Health 2016.05.27 Tokyo
Medical and Dental University
10. Takashi Zaitsu. Space Dentistry –Teledental System in Space and Antarctic Environments-, The 12th
International Conference of Asian Academy of PreventiveDentistry.. The 12th International Conferenceof Asian Academy of PreventiveDentistry. Joint meeting with The 65th General Meeting of JapaneseSociety for Oral Health 2016.05.27 Tokyo Medical and Dental University
11. Nguyen Thi Hoang Yen, Takashi Zaitsu, Sachiko Takehara, Masayuki Ueno, Yoko Kawaguchi. Early
childhood caries and related risk factors in Vietnam, The 12th International Conference of Asian Academyof PreventiveDentistry.. The 12th International Conference of Asian Academy of PreventiveDentistry.
Joint meeting with The 65th General Meeting of Japanese Society for Oral Health 2016.05.27 Tokyo
Medical and Dental University
12. Kaung Myat Thwin, Takashi Zaitsu, Masayuki Ueno, Yoko Kawaguchi. Early childhood caries and related
risk factors among Myanmar preschool children,. The 12th International Conference of Asian Academy ofPreventiveDentistry. Joint meeting with The 65th General Meeting of Japanese Society for Oral Health2016.05.27 Tokyo Medical and Dental University
13. Anastasia Blizniuk,Takashi Zaitsu, Masayuki Ueno, Yoko Kawaguchi. Oral Health Literacy and its impact
on oral health in Belarusian adults. The 12th International Conference of Asian Academy of PreventiveDentistry. Joint meeting with The 65th General Meeting of Japanese Society for Oral Health 2016.05.27
Tokyo Medical and Dental University
14. Zaitsu T, Kawaguchi Y. Oral health status on of employees classiﬁed by work schedule. IADR/APR
General Session & Exhibition 2016.06.23 Seoul, Republic of Korea― 156 ―

Public HealthPublic Health

15. Yen Hoang Thi Nguen,Masayuki Ueno,Takashi Zaitsu,Yoko Kawaguchi. Early Childhood Caries of PreschoolChildren in Hue,Vietnam. The 23rd General Meeting of The Japanese Association for Dental Science2016.10.21 Fukuoka international Congress Center
16. Takashi Zaitsu, Yoko Kawaguchi. Teledental System in the Environments of Space and Antarctica. The
5th Tri-University Consortium on Oral Science and Education 2016.10.27 China― 157 ―

Public HealthPublic HealthSports Medicine and Dentistry[ Associate Professor] Toshiaki Ueno
[ Assistant Professor] Toshiyuki Takahashi, Hiroshi Churei[ Clinical fellow] Katsuhide Kurokawa[ Graduate Student] Abhishekhi Shrestha, Mai Ikegawa, Takahiro Sirako, Yuriko Yoshida, Nana Shiota,
Gen Tanabe, Kiyonobu Yamamura, Chiho Shibata, Phyu Sin Tun[ Research Student] Satoshi Kawabata, Aki Kanasaki[ Part-time Instructor] Goshi Kondo, Yukio Sasaki, Ryo Sato, Takuto Yamanaka,Sachiko Fujino[ Part-time Resident] Hiromi Miura, Koichiro Adachi, Keisuke Abe, Kenji Takeuchi, Takayuki Ishigami,
Kairi Hayashi, Takaaki Fukuda，Akihiro Mitsuyama,Sintaro Fukasawa,(1)

OutlineSport medicine/dentistry is a branch of clinical medical and dental sciences which deals with the clinical management of oral health of athletes and sports-active people, the safety measures of sports-related traumatic
injuries and disorders, and medical and dental supports to improve athletic performance.
(2)

Research

1) Oral health promotion of athletes and sports-active people(1) Field survey of oral health conditions in athletes and sports-active people(2) Changes of oral environment associated with physical and sporting activities(3) Inﬂuences of sports drinks and supplements on oral health2) Safety measures of sports-related dental and maxillofacial traumatic injuries(1) Diagnosis and treatment techniques for sports-related dental and maxillofacial injuries(2) Development and innovation of sports mouthguard(3) Development and innovation of sports faceguard(4) Development and innovation of scuba diving mouthpiece3) Correlations between occlusion and general motor functions(1) Biomechanical assessment of motor performance associated with occlusion(2) Electrophysiological analysis of neuromuscular function associated with occlusion
4) Correlations between occlusion and body posture(1)Eﬀect of occlusion on static posture(2)Inﬂuence of occlusion on dynamic posture5) Relations between mastication and occlusion and brain functions
6) Application of HBO therapy to sports-related dental diseases and traumatic injury(3)

Education

academic classes for sports medicine/dentistryin undergraduate and graduate courses in undergraduate andgraduate courses are listed as follows;
1)D1:Shigaku-Gaisetu
2)D3:Sogo-Kadai-Ensyu
3)D3:Rinsyo-Taiken-Jissyu― 158 ―

Public HealthPublic Health4)D4:Kenkyu-Taiken-Jissyu
5)D5:Hatten-Shika-Rinsyo/Sports Dentistry6)D5-D6:Hokatsu Rinsyo-Jissyu Phase I to II
7)OH2:Kiso-Kagaku-Jissyu
8)OH3:Seijin-Koukuhoken-Eiseigaku
9)OH3-OH4:Koukuhoken-Eisei-Jissyu
10)OE4:Sports dental engineering11)Master course:Kankyo-Syakai-Ishigaku
12)PhD course: Tokuron, Ensyu and Kenkyu-Jissyu of Sports Medicine and Dentitry13)Clinical training course:Sports Dentistry(4)

Lectures & Courses

Main objectives of academic education programs of sports medicine/dentistry in from undergraduate to graduate courses is to provide the students to study the oral health conditions in athletes and sports-active people,
the changes of oral environment associated with physical and sporting activities, the possible correlations between occlusion and general motor functions and body posture, the novel techniques of sports mouthguard
and faceguard, the relations between mastication and occlusion and brain functions, and so on. Students arealso taught to advanced knowledge on sports medicine/dentistry and up-to-date techniques to fabricate custom
mouthguard and faceguard.

(5)

Clinical Services & Other WorksSports dentistry clinic in Dental Hospital of Tokyo Medical and Dental University oﬀers comprehensive careand clinical management for athletes and sports-active people suﬀered dental diseases and traumatic injuries.
Custom-ﬁtted protective gears such as mouthguard and faceguard against sports-related dental and maxillofacial
trauma are also handled for participants in contact sports such as a boxing, American football, rugby football,hockey, lacrosse, and martial art. Sports dentistry clinic is positioned as a dental branch of Clinical Center forSports Medicine and Dentistry under TMDU Sports Science Organization. In addition, Our clinic maintains
close cooperation with Japan Institute of Sports Science/National Training Center under Japan Sport Council.

(6)

Clinical Performances< Certiﬁed specialists>
Toshiaki Ueno (1.JASA Sports Dentist certiﬁed by Japan Sports Association, 2.Dental specialist certiﬁed by
Japanese Academy of Sports Dentistry, 3.Mouthguard technical instructor certiﬁed by JASD, 4.Dental specialist
certiﬁed by Japanese Academy of Maxillofacial Prosthetics)
Toshiyuki Takahashi (1.MG technical instructor certiﬁed by JASD)
Hiroshi Churei (1.JASA Sports Dentist certiﬁed by Japan Sports Association, 2.Dental specialist certiﬁed by
JASD, 3. MG technical instructor certiﬁed by JASD)
Katsuhide Kurokawa (1.MG technical instructor certiﬁed by JASD)
Sachiko Fujino (1.Dental specialist certiﬁed by JASD, 2.MG technical instructor certiﬁed by JASD)
Keisuke Abe (1.JASA Sports Dentist certiﬁed by Japan Sports Association, 2.Dental specialist certiﬁed by
JASD, 3.MG technical instructor certiﬁed by JASD)
Takayuki Ishigami (1.MG technical instructor certiﬁed by JASD)
Kairi Hayashi (1.MG technical instructor certiﬁed by JASD)
Akihiro Mitsuyama (1.MG technical instructor certiﬁed by JASD, 2.Dental specialist certiﬁed by Japan Association of Dental Traumatology)
Shintaro Fukasawa (1.MG technical instructor certiﬁed by JASD, 2.Dental materials adviser certiﬁed by JapaneseSociety of Dental Materials and Devices)
Mai Tanabe (1. Sports dental hygienist certiﬁed by JASD)
Takahiro Shirako(1.MG technical instructor certiﬁed by JASD, 2.Dental specialist certiﬁed by JADT)Aki Kanasaki(1. Sports dental hygienist certiﬁed by JASD)― 159 ―

Public Health(7)

Public HealthPublications[Original Articles]
1. Hayashi K, Takahashi T, Tanabe M, Horie M, Oyaizu T, Enomoto M, Shibata S, Yagishita K, Ueno T.
The facilitatory eﬀects of hyperbaric oxygen treatment on membrane bone wound healing in a rat calvarial
defect model Undersea Hyperb Med. 2016.03; 43(2); 135-142
2. Mitsuyama A, Takahashi T, Ueno T. Eﬀects of teeth clenching on soleus H reﬂex during lower limb muscle
fatigue J Prosthodontic Res. 2016.06; Epub ahead of print
3. Fukasawa S, Churei H, Chowdhury RU, Shirako T, Shahrin S, Shrestha A, Wada T, Uo M, Takahashi
H, Ueno T. Application of addition-cured silicone denture relining materials to mouthguard adjustment
Dent Mater J. 2016.07; 35(4); 635-643
4. Shrestha A, Takahashi T, Kurokawa K, Mitsuyama A, Hayashi K, Ishigami T, Shahrin S, Chowdhury
RU, Churei H, Ueno T. Eﬀects of mouthguards on electromyographic activity of masticatory muscles Int
J Sports Dent. 2016.10; 9(1); 27-37
5. Ishigami T, Takahashi T, Kurokawa K, Ueno T. Eﬀects of occlusal supporting area on dynamic postureagainst disturbance by electrical stimulation Int J Sports Dent. 2016.10; 9(1); 7-17
6. Takahiro Wada, Hiroshi Churei, Toshiaki Ueno, Motohiro Uo. High shock absorbing faceguard usingﬁber-reinforced plastic (FRP) and elastomer The Journal of the Japanese Society for Dental Materials
and Devices. 2016.11; 35(6); 325-328
7. Nakamura T, Yoshida Y, Churei H, Aizawa J, Hirohata K, Ohmi T, Ohji S, Takahashi T, Enomoto M,
Ueno T, Yagishita K. The eﬀect of teeth clenching on dynamic balance at jump-landing: a pilot study J
Appl Biomech. 2016.12; Epub ahead of print
8. Fukasawa S, Churei H, Chowdhury RU, Shahrin S, Shrestha A, Wada T, Uo M, Takahashi H, Ueno T.
Diﬀerences among shock-absorbing capabilities of mouthguard materials Dent Traumatol. 2016.12; 32(6);474-479[Conference Activities & Talks]
1. Ikegawa M, Takahashi T, Ueno T. Eﬀects of water gargling on changes of saliva pH and buﬀering capacity
by taking sports drinks during exercise. 7th International Conference of Sports & Society 2016.06.02
Hawaii, U.S.A.
2. Takahiro Wada, Hiroshi Churei, Shintaro Fukasawa, Takahiro Shirako, Toshiaki Ueno, Motohiro Uo.
Shock-absorbing property of face guard by incorporating a glass-ﬁber reinforced plastic and space. 2016.06.12
3. Shirako K, Churei H, Ueno T. Splinting by use of novel luxated tooth model and Periotest. 19th World
Congress on Dental Traumatology 2016.08.11 Brisbane, Australia
4. Takahiro Wada, Hiroshi Churei, Haruka Takayanagi, Mako Yokose, Shitaro Fukasawa, Takahiro Shirako,
Toshiaki Ueno, Naohiko Iwasaki, Hidekazu Takahashi, Motohiro Uo. Development of a faceguard using a
glass-ﬁber reinforced thermoplastic (GFRTP) and shock escape space. Visual-JW 2016 2016.10.17 Suita,
Osaka, Japan
5. Takahiro Wada, Hiroshi Churei, Shintaro Fukasawa, Takahiro Shirako, Toshio Hongo, Toshiaki Ueno,
Motohiro Uo. Analysis of shock absorption ability of a faceguard by incorporating a glass-ﬁber reinforced
thermoplastic and space. International Dental Materials Congress 2016 2016.11.05 Bali, Indonesia
6. Tanabe G, Churei H, Wada T, Uo M, Takahashi H, Ueno T. Eﬀect of the use of commercial cleaning
liquid in thermoforming process of mouthguard materials. International Dental Materials Congress 20162016.11.05 Bali, Indonesia― 160 ―

Public Health
HealthPublicEducational Media Development

Professor KINOSHITA Atsuhiro
Assistant Professor SUNAGA Masayo
Graduate Student MIYOSHI Tomoe
Graduate Student HOBO Koki
Graduate Student CAO Ridan
Graduate Student AKIYAMA Kyoko
Graduate Student TAKATSUNA Yukiko(1)

Research

1) Development of computer-assisted clinical simulation system for medical and dental practice training.
In our university, we executed the Establishment of Computer-Assisted Education System on Clinical Simulation
for Medical and Dental Practice Training project, which was adopted as part of the Support Program forDistinctive University Education in 2005, and developed the computer simulation materials on clinical education
by utilizing digital clinical data from our Medical and Dental hospitals. We have expanded our study into a
new project, ’Progress of Computer-Assisted Simulation for Medical and Dental Practice Training. ComputerAssisted Simulation Promoting Clinical Inference, Decision-making, Problem Solving and Cooperation Abilities
of Health Professionals’, which was subsequently selected to be part of the Program for Promoting the University
Education Reform in 2009 by the Ministry of Education, Culture, Sports, Science and Technology. After utilizing
the simulation materials for our students, we will evaluate and analyze their educational eﬃcacy. Furthermore,
we will develop a computer-assisted clinical simulation system for the entire university.
2) Development of new education system using information and communication technologies for medical anddental students.
At our university, we executed the Integration of Information and Communication Technologies into ClinicalTraining project, which was adopted as part of the Support Program for the Contemporary Educational Needs
in 2007. The aim of this program is to integrate traditional educational methods with advanced information andcommunication technologies in order to allow clinical training, practical training and lectures to be eﬀectively
interlinked. By expanding digital content and employing an automatic visual recording system, we are planningto establish a digital archive of treatments and surgeries, demonstrations of dental techniques, lectures andstudent training. We will then launch an on-demand distribution system in order to incorporate this content
into clinical education, which the students will be able to use for their self-evaluation and learning.
3) Development and utilization of an educational media for medical and dental students.
• Development and Study of Computerized Dental Simulator for Training of Dental Cavity Preparation and
Prosthodontic Tooth Preparation practices:
We plan to develop a new computerized dental simulator and evaluate its eﬀectiveness for training in dental
cavity preparation and prosthodontic tooth preparation.
• Development and Study of Dental Model and Kit for Practical Training:
Dental and dental hygiene students must acquire skills for measuring periodontal pockets and must learn to
identify the base of the pocket. However, few dental models are commercially available, and students cannot
measure deep periodontal pockets by practicing on one another. Thus, we developed a new dental model withwhich the students can practice the probing of deep periodontal pockets, and plan to evaluate its eﬀectivenessin training and evaluation of examiners.
• Development of Composing and Screening System for Original 3D Movies from Operator’s Viewpoint:― 161 ―

Public HealthPublicHealthIf students can experience and recognize three-dimensional space from the operator’s (instructor’s) viewpoint
during their practice sessions and lectures, it would have educational beneﬁts. Thus, we plan to develop a
Composing and Screening System for Original 3D Movies from an Operator’s Viewpoint. Furthermore, we will
improve the quality of distance learning and remotely operated instruction using the superimposing method.
• Development of Dental Handpiece System with CCD camera:
We plan to develop a system equipped with a CCD camera, mirror and reverse image units in order to allow
students in the lecture room to observe dental treatment sites in real-time, thereby giving them a sense of being
at a clinic.

(2)

Education

We will assist graduate students in understanding new educational systems and media utilizing informationcommunication technologies, such as the computer-assisted education system, the e-learning system and thelive broadcasting lecture system. We will also assist these students in mastering how to create related educational media and apply it to medical, dental, nursing and dental hygiene education, as well as interprofessional
cooperation.
First-year students at the School of Dentistry, and ﬁrst and second-year students at the School of Oral HealthCare Sciences will learn to process media information and create media content, as well as how to search theInternet for information that is necessary for their study and research activities. They will also learn how tomake use of various databases.
Fourth-year students at the School of Dentistry will acquire the practical knowledge, communication skills andattitude to build good relationships with patients by gaining clinical experience at an early stage. This practiceconsists of two units; clinical experience in the teaching clinic and the computer-assisted simulation practice.
This experience will enhance the students abilities, enabling them to be eﬀective clinicians.

(3)

Publications[Original Articles]
1. Yuka Tsumanuma, Takanori Iwata, Atsuhiro Kinoshita, Kaoru Washio, Toshiyuki Yoshida, Azusa Yamada, Ryo Takagi, Masayuki Yamato, Teruo Okano, Yuichi Izumi. Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size SupraAlveolar Periodontal Defect Model. Biores Open Access. 2016.01; 5(1); 22-36
2. Michiyo Kurosa, Ken-ichi Tonami, Satoko Ohara, Sachi Umemori, Kanako Noritake, Masayo Sunaga,
Atsuhiro Kinoshita, Kouji Araki. Diﬀerences between Computer-Based and Paper-Based Assessments of
the Clinical Reasoning Competency of Dental Students. Kokubyo Gakkai Zasshi. 2016.03; 83(1); 25-33
3. Naoko Seki, Janelle Moross, Masayo Sunaga, Koki Hobo, Tomoe Miyoshi, Hiroshi Nitta, Atsuhiro Kinoshita, Ikuko Morio. Evaluation of simulation learning materials use to ﬁll the gap in Japanese dental
English education. J. Med. Dent. Sci.. 2016.03; 63(1); 1-8
4. Hiromi Otsuka, Keiko Kondo, Yuki Ohara, Masayo Yasuda, Natsuki Kishimoto, Masayo Sunaga, KeikoEndo, Shinichi Arakawa, Atsuhiro Kinoshita, Kayoko Shinada. An Inter- and Intraprofessional Education
Program in Which Dental Hygiene Students Instruct Medical and Dental Students. J Dent Educ. 2016.09;
80(9); 1062-1070
5. Masayo Sunaga, Masato Minabe, Koji Inagaki, Atsuhiro Kinoshita. Eﬀectiveness of a Specially Designed
Dental Model for Training, Evaluation, and Standardization of Pocket Probing. J Dent Educ. 2016.12;80(12); 1430-1439― 162 ―

Public Health
HealthPublicInsured Medical Care Management

Professor Masumi AI
J Associate Professor Junichiro ISHIOKA
Graduate Student Hiroshi KAWAMURA(1)

OutlineOur department supports an appropriate practice on insured medical care and billing for medical service feesat the TMDU medical hospital.
We also focus on development of methodology and materials for education on medical insurance system andrules for insured medical treatment.

(2)

Research

1) Development of methodology and materials for education on medical insurance system and rules for insured
medical treatment.
2) Studies on management and supports for billing for medical service fees at insurance medical institutions.
3) Studies on aﬀairs of medical insurance system and provision of medical services.
In addition, the staﬀ has been engaged in clinical studies and epidemiological studies on lipid metabolism,
diabetes mellitus, atherosclerosis, laboratory medicine, and urology.

(3)

Education

The staﬀ has been in charge for education of social health insurance system and rules for insured medical
treatment at the TMDU medical hospital (May 2016 and February 2017).
A doctor course student is in his second year.

(4)

Lectures & Courses*Providing practical supports for an appropriate insured medical care in the clinical ﬁelds.
Providing individual support for an appropriate billing for medical service fees at the medical hospital.
We also focus on development of methodology and materials for education on medical insurance system andrules for insured medical treatment.

(5)

Clinical Services & Other Works

The staﬀ has been in charge for assisting appropriate medical fee claims, and also providing clinical service on
diabetes, dyslipidemia, atherosclerosis, geriatrics, and Urology.

― 163 ―

Public HealthPublicHealth(6)

Clinical Performances

As a managing section of the medical hospital, we collaborate all kinds of hospital workers practically andeﬃciently to provide an appropriate insured medical care.
(7)

Publications[Original Articles]
1. Eiichiro Kanda, Masumi Ai, Mitsuyo Okazaki, Masayuki Yoshida, Yoshitaka Maeda. Association of HighDensity Lipoprotein Subclasses with Chronic Kidney Disease Progression, Atherosclerosis, and Klotho.
PLoS ONE. 2016; 11(11); e0166459
2. Yoh Matsuoka, Noboru Numao, Kazutaka Saito, Hiroshi Tanaka, Jiro Kumagai, Soichiro Yoshida, Junichiro Ishioka, Fumitaka Koga, Hitoshi Masuda, Satoru Kawakami, Yasuhisa Fujii, Kazunori Kihara.
Candidate selection for quadrant-based focal ablation through a combination of diﬀusion-weighted magnetic resonance imaging and prostate biopsy. BJU Int.. 2016.01; 117(1); 94-101
3. Takayuki Nakayama, Noboru Numao, Soichiro Yoshida, Junichiro Ishioka, Yoh Matsuoka, Kazutaka Saito,
Yasuhisa Fujii, Kazunori Kihara. A novel interactive educational system in the operating room–the IE
system. BMC Med Educ. 2016.02; 16; 44
4. Naoko Kawamura, Minato Yokoyama, Yasuhisa Fujii, Junichiro Ishioka, Noboru Numao, Yoh Matsuoka,
Kazutaka Saito, Chizuru Arisawa, Tetsuo Okuno, Akira Noro, Shinji Morimoto, Kazunori Kihara. Recovery of renal function after radical nephrectomy and risk factors for postoperative severe renal impairment:
A Japanese multicenter longitudinal study. Int. J. Urol.. 2016.03; 23(3); 219-223
5. Hiroaki Ikezaki, Masumi Ai, Ernst J Schaefer, Seiko Otokozawa, Bela F Asztalos, Katsuyuki Nakajima,
Yanhua Zhou, Ching-Ti Liu, Paul F Jacques, L Adrienne Cupples, Norihiro Furusyo. Ethnic Diﬀerencesin Glucose Homeostasis Markers between the Kyushu-Okinawa Population Study and the Framingham
Oﬀspring Study. Sci Rep. 2016.11; 6; 36725
6. Yoshio Fujitani, Shimpei Fujimoto, Kiyohito Takahashi, Hiroaki Satoh, Takahisa Hirose, Toru Hiyoshi,
Masumi Ai, Yosuke Okada, Masahiko Gosho, Tomoya Mita, Hirotaka Watada. Eﬀects of linagliptin
monotherapy compared with voglibose on postprandial blood glucose responses in Japanese patients with
type 2 diabetes: Linagliptin Study of Eﬀects on Postprandial blood glucose (L-STEP). Diabetes Res.
Clin. Pract.. 2016.11; 121; 146-156[Misc]
1. Matsuoka Y, Inoue M, Ito M, Yoshida S, Yokoyama M, Ishioka J, Numao N, Saito K, Fujii Y, Kihara K.
Gassless single-port RoboSurgeon radical prostatectomy using an endoscopic manipulator robot 2016.07;
8(1); 31-35[Conference Activities & Talks]
1. Yoshida S et al. . Potential utility of standardized apparent diﬀusion coeﬃcient value as a biomarker
predicting clinical aggressiveness of bladder cancer with various MRI protocols at various institutions.
European Association of Urology annual congress 2016.03.12 Munich, Germany
2. Tanaka H et al. Novel stepwise algorithm using CT and MRI for diﬀerential diagnosis of fat-poor angiomyolipoma in small renal masses: Development and external validation. European Association of
Urology annual congress 2016.03.12 Munich, Germany
3. Ishioka J et al. When should patients undergo prostate biopsy? Decision analysis using diﬀerences in
the health-related quality-of-life between pre-biopsy healthy men and patients with castration-resistant
prostate cancer. European Association of Urology annual congress 2016.03.12 Munich, Germany
4. Fujiwara M et al.. Antibiotic prophylaxis is avoidable in minimally invasive clean surgery for renal or
adrenal tumors: A prospective study of 678 cases. Munich, Germany 2016.03.12 Munich, Germany― 164 ―

Public Health
HealthPublic5. 1.Fujii Y, Kihara K, Tanaka H, Saito K., Yoshida S, Yokoyama M., Ishioka J, Matsuoka Y, Numao N.
. Bladder-sparing protocol consisting of ow-dose chemoradiotherapy and consolidative partial cystectomy
against muscle-invasive bladder cancer: Oncological and functional outcome in elderly patients..
European Association of Urology annual congress 2016.03.13 Germany
6. 5.Yokoyama M, Kawamura N, Fujii Y, Inoue M, Ishioka J, Numao N, Matsuoka Y, Saito K, Arisawa
C, Okuno T, Noro A, Morimoto S, Kihara K. . Longitudinal changes in renal function after radical
nephrectomy and risk factors for postoperative severe renal impairment: A Japanese multicenter study
using a linear mixed model analysis. European Association of Urology annual congress 2016.03.13 Germany
7. Moriyama S et al. A new quantitative method for characterizing small renal masses: MRI intensity ratio
curve analysis. European Association of Urology annual congress 2016.03.13 Munich, Germany
8. Waseda Y. et al.. Elevation of urinary metanephrine/normetanephrine and impairment of glucose
toler-ance predict the development of hypoglycemia after resection of pheochromocytoma.
European Association of Urology annual congress 2016.03.13
9. Nakamura Y et al. Renal dysfunction manifestation in patients with adrenal Cushing’s syndrome after
adrenalectomy. European Association of Urology annual congress 2016.03.13 Munich, Germany
10. 4.Kawamura N, Yokoyama M, Fujii Y, Inoue M, Ito M, Yoshida S, Ishioka J, Numao N, Matsuoka Y,
Saito K, Kihara K.. Longitudinal preservation of renal function after clamp-less partial nephrectomy: An
analysis of patients with chronic kidney disease.. European Association of Urology annual congress2016.03.14 Germany
11. 14.Yasuda Y, Saito K, Kawamura N, Yuasa T, Yokoyama M, Matsuoka Y, Ishioka J, Numao N, Okuno
T, Yamamoto S, Takahashi S, Yonese J, Fujii Y, Kihara K. Prognostic signiﬁcance of early CRP response
for metastatic renal cell carcinoma treated with tyrosine kinase inhibitor. European Association of
Urology annual congress 2016.03.14 Germany
12. Matsuoka Y, Ishioka J, Tanaka H, inoue M, Ito M, Yoshida S, Yokoyama M, Numao N, Saito K, Fujii Y,
Kihara K. Impact of PI-RADS version 2 on MRI diagnosis for extracapsular extension of prostate cancer:
A multireader study. European Association of Urology annual congress 2016.03.14 Munich, Germany
13. Inoue M et al. . Prediction of therapeutic resistance using ERBB2 expression status and Ki-67 labelling
index in muscle-invasive bladder cancer patients treated with chemoradiation-based selective bladdersparing approach. European Association of Urology annual congress 2016.03.14 Munich, Germany
14. Matsuoka Y, Numao N, Saito K, Tanaka H, Inoue M, Ito M, Yoshida S, Yokoyama M, Ishioka J, Fujii
Y, Kihara K. Applicability analysis of focal therapy to intermediate- and high-risk prostatecancer. .annual meeting of the American Urological Association 2016.05.06 San Diego, USA
15. Numao N et al. Optimal combination of MRI-targeted biopsy and systematic biopsy for men with suspicionof prostate cancer. annual meeting of the American Urological Association 2016.05.06 San diego, USA
16. Ishioka J et al.. The incidence of late bladder tumor recurrence of upper tract urothelial carcinoma andoptimal cystoscopic follow-up: A multicenter study.. annual meeting of the American Urological
Association 2016.05.06 San diego, USA
17. Nakamura Y et al.. Masked renal dysfunction in patients with adrenal Cushing’s syndrome manifested
by adrenalectomy. annual meeting of the American Urological Association 2016.05.06 San diego, USA
18. Tanaka H et al. Development and validation of a novel stepwise algorithm using CT and MRI for
diagnosis of fat-poor angiomyolipoma in small renal masses. .annual meeting of the American Urological
Association 2016.05.07― 165 ―

Public HealthPublicHealth

19. Ito M et al. Likelihood of underlying Lynch syndrome in Japanese upper tract urothelial carcinoma
patients. annual meeting of the American Urological Association 2016.05.07 San diego, USA,
20. Kobayashi M et al. Maximum standardized uptake value of 18F-FDG PET/CT predicts survivalof patients with advanced urothelial carcinoma. annual meeting of the American Urological Association
2016.05.07 San diego, USA
21. Fujii Y et al.. Bladder-sparing protocol consisting of low-dose chemoradiotherapy and consolidative partial
cystectomy against muscle-invasive bladder cancer: Comparison of oncological and functional outcomes
between older and younger patients. annual meeting of the American Urological Association
2016.05.08 USA
22. Ishioka J et al. Prognostic impact of C-reactive protein kinetics on survival of patients with advanced
urothelial carcinoma. A multicenter study. . annual meeting of the American Urological Association
2016.05.08 San diego, USA
23. 8.Ishioka J et al. Yokoyama M., Kawamura N., Fujii Y., Inoue M., Ishioka J., Numao N., Matsuoka
Y., Saito K., Arisawa C., Okuno T., Noro A., Morimoto S., Kihara K.. Longitudinal renal function
recovery after radical nephrectomy and risk factors for postoperative severe renal impairment: a Japanesemulticenter study.. annual meeting of the American Urological Association 2016.05.08 San diego, USA
24. Matsuoka Y, Ishioka J, Tanaka H, Inoue M, Ito M, Yoshida S, Yokoyama M, Numao N, Saito K, Fujii Y,
Kihara K. Impact of the Prostate Imaging Reporting and Data System version 2 (PI-RADS v2) on theassessment of extracapsular extension in prostate cancer: A multireader study.. annual meeting of theAmerican Urological Association 2016.05.09 San Diego, USA
25. 13.Nakayama T, Kazutaka S, Fujii Y, Waseda Y, Moriyama S, Tanaka H, Kawamura N, Inoue M, Ito M,
Yoshida, S, Yokoyama M, Matsuoka Y, Numao N, Kihara K. Association of M2 macrophage invasion
with C-reactive protein in renal cell carcinoma.. annual meeting of the American Urological Association
2016.05.09 San diego, USA
26. Masaharu Inoue, Fumitaka Koga, Soichiro Yoshida, Hajime Tanaka, Shuichiro Kobayashi, Minato Yokoyama,
Junichiro Ishioka, Yoh Matsuoka, Noboru Numao, Kazutaka Saito, Yasuhisa Fujii, Kazunori Kihara.
Predictive model for therapeutic resistance using ERBB2 expression status and Ki-67 labeling index inmuscle-invasive bladder cancer patients treated with chemoradiotherapy-based bladder-sparing protocol.
AUA2016 Annual Meeting 2016.05.09 San Diego, USA
27. Matsuoka Y, Saito K, Numao N, Tanaka H, Inoue M, Ito M, Yoshida S, Yokoyama M, Ishioka J, Fujii
Y, Kihara K. Risk assessment for undertreatment in focal therapy and its applicability to intermediateand high-risk prostate cancer. 9th International Symposium on Focal Therapy and Imaging in prostate& kidney cancer 2016.06.23 Durham, USA
28. Saito K, Matsuoka Y, Numao N, Hayashi K, Toda K, Ito M, Inoue M, Yoshida S, Yokoyama M, Ishioka J,
Fujii Y, Yoshimura R, Kihara K. Hemi-gland brachytherapy for localized prostate cancer. 9th International
Symposium on Focal Therapy and Imaging in prostate & kidney cancer 2016.06.23 Durham, USA[Others]
1. IRB Member, Tokyo Institute of Technology 20112. IRB Member, Sony Corporation 2012-― 166 ―

Public Health
HealthPublicDepartmentofHealth
GlobalEntrepreneurship
Health Entrepreneurship
GlobalProfessor: Keiko Nakamura, MD, PhD
Junior Associate Professor: Kaoruko Seino, PhD
International Researcher: Layla McCay, MBChB
Graduate Student: Nguen Huu Chau Duc, MD; Rakprasit Jutarat, MPH;
Shagdarsuren Tserendulam,MA; Saber Al-Sobaihi, MPH;
Aya Anzai,RN; Omar Mohammad Mashal,MD;
Shiro Ochi, MSc,PhD; Tomoko Terada, MD,
Dasavanh Manivong, MD, MSc; Delgermaa Doshzeveg, MPH;
Iskander Isaac Maro, MD, MPH; Yoshiko Shimozawa, PT, MMs;
Hoang Thuy Linh Nguyen, MD, MPH; Ahmad Shekib Arab,MD;
Yuri Tashiro,MPharm,MPH;Deogratius Bintabara MD, MSc;
TJ Robionson Moncatar, RN, MPH; Kathryn Lizbeth Lucena Siongco, RN, RM, MAN(1)

Outline

The department of Global Health Entrepreneurship seeks to elucidate physical, social, economic and cultural
factors determining inequity in health. The department works closely with WHO and other international
agencies to help develop guidelines of scientiﬁc evaluation and recommended practices.

(2)

Research

Major Research Topics:
1) Transﬁguration of the ecosystem and its interaction with human health2) Socio-cultural factors determining health3) Social entrepreneurship development through applying the Healthy Settings approach4) Use of information technology to improve public health5) International health workforce and trade in health services6) Universal health covrage in ageing society(3)

Education

PhD programs
Our doctoral program provides a ﬂexible curriculum that allows students to customize their research goals,
methods, and activities based upon their own interests and preferences. Students on the Public Health Medicine(PHM) track of the Disease Prevention Global Leader Program (DP-GLP) attain the skills required for publichealth professionals with an international perspective. The program prepares them for leadership roles in public
institutions. Advanced students from many countries around the world are now enrolled. All the classes are
conducted in English, thus facilitating the acquisition of international communication skills.
A rich variety of educational activities have been arranged in the program. These include: individual discussion
sessions with professors and other faculty members; ﬁeld investigations; and seminars on various topics suchas community health care, community medicine, public health policy, biostatistics, academic presentation,
development of foreign language skills, and communication skills. Students work closely with faculty members
on an individual basis in setting the right direction for their research and conﬁrmation of their progress.

― 167 ―

Public HealthPublicHealthMaster Programs
Master degree students receive systematic intensive training that leads to the acquisition of broad expertise in
the ﬁeld of global public health. This program is open to students who have majored in any ﬁeld.

(4)

Lectures & Courses

The objective of our postgraduate education is to provide professional qualiﬁcations to high-caliber people
who exhibit leadership in the advancement of public health and promotion of health on an international scale.
The department helps students attain the knowledge, skills, attitude, and experiences that are necessary forcompetent health specialists and social entrepreneurs in healthcare.
By completion of the doctoral course, the participants are expected to be able to:•Assess health and well being the populations in local, national, and international settings,•Assess evidence to show eﬀectiveness of health interventions, programs and strategies,•Think strategically to develop local, national, and international policies,•Manage projects to successful completion•Demonstrate leadership in local, national, or international public health programs•Communicate properly when listening, presenting, writing, and negotiating•Pursue a full-cycle of an academic, public health research project•Facilitate learning of staﬀ, students, and colleagues, and•Practice and respect professional ethics in a socio-culturally diverse environment.

(5)

Publications[Original Articles]
1. Mashal MO, Nakamura K, Kizuki M. Hidden burden of non-medical spending associated with inpatientcare among the poor in Afghanistan. International Journal of Public Health. 2016.05; 61; 661-671
2. Rami Al Rifai, Keiko Nakamura, Kaoruko Seino. Decline in the prevalence of anaemia among children ofpre-school age after implementation of wheat ﬂour fortiﬁcation with multiple micronutrients in Jordan.
Public Health Nutr. 2016.06; 19; 1486-1497
3. Nakamura K, Chiba M, Seino K. Factors inﬂuencing reduction of risk of lifestyle related disease amongchildren. 2016.08; 31; 381-386
4. Shaﬁqullah H,Morita A,Nakamura K, Seino K . The family planning conundrum in Afghanistan Health
Promotion International. 2016.10;
5. Al-Sobaihi S, Nakamura K, Kizuki M.. Undernutrition among children under 5 years of age in Yemen: Roleof adequate childcare provided by adults under conditions of food insecurity. Journal of Rural Medicine.
2016.11; 11(2); 47-57[Conference Activities & Talks]
1. Nakamura K. Post-disaster excess deaths of NCD and Disaster Health Emergency Assistance Team..
WHO Meeting on Healthy and Resilient Cities 2016.01.11 Gandong-gu, Seoul, Republic of Korea
2. Nakamura K. Healthy Cities and Food is Asia. HCSO-AFHC Symposium 2016.02.02 Tokyo
3. Nakamura K. Examples of actions to address urban health challenges: Urban health system roles andfunctions: Emergency Preparedness Plan. Meeting on Implementation of the Regional Framework for
Urban Health in the Western Paciﬁc (2016-2020) 2016.02.29 Manila, Philippines
4. Nakamura K. Health Supportive City Development. The 12th Annual Conference of the Japan Chapterof the Alliance for Healthy Cities 2016.07.26― 168 ―

Public Health
HealthPublic5. Nakamura K. Public Health. Graduate School Seminar at School of Public Health, MUHAS 2016.08.03
Dar es Salaam, Tanzania
6. Nakamura K, Fawkes S. The Foresight Approach towards Healthy and Resilient Cities. The 7th Global
Conference of the Alliance for Healthy Cities 2016.08.29 Wonju, Republic of Korea
7. Nakamura K. Nationwide program for prevention and control of lifestyle related disease, Japan.. 8th NCD
Forum Seminar 2016.09.28 Seoul, Republic of Korea― 169 ―

Gerontology and Gerodontology

Gerontology and Gerodontology

Geriatrics and Vascular Medicine

Professor：Kentaro SHIMOKADO
Associate Professor：Eiji KANEKO, Shohei SHINOZAKI
Junior Associate Professor：Masashi BEPPU
Assistant Professor：Yasuko ABE
Graduate Student：Kenji TOYOSIMA, Norihiko IZUMIMOTO
Yasuko USHIO, I, Hirofumi MASUTOMI
Tomomi HAKAMADA, Marie NAKAMURA
Rie MASUDO, Suguru MABUCHI, Mari SASAKI(1)

Research

1) Cell biological mechanisms of atherogenesis
2) Mechanisms involved in dyslipidemia
3) Mechanisms of aging and age-related diseases
4) Undergraduate and postgraduate education in geriatrics(2)

Education1) Undergraduate education of medical students with a particular emphasis on geriatrics
2) Development of research on aging and age-related diseases with a particular emphasis on atherosclerosis(3)

Clinical Services & Other Works

As a division of the Department of Internal Medicine, we are taking care of elderly patients who are better
treated by us rather than by highly specialized experts both at the outpatient clinics and the ward of ouruniversity hospital. We also provide subspecialty service such as oriental herbal medicine, mononuclear celltransplantation for PAD, and dyslipidemia clinic.
(4)

Publications[Original Articles]
1. Takahashi Keita, Takisawa Shoko, Shimokado Kentaro, Kono Nozomu, Arai Hiroyuki, Ishigami Akihito.
Age-related changes of vitamin E: alpha-tocopherol levels in plasma and various tissues of mice and hepaticalpha-tocopherol transfer protein. Eur J Nutr. 2016.02;
2. Sasaki Mari, Shinozaki Shohei, Shimokado Kentaro. Sulforaphane promotes murine hair growth by accelerating the degradation of dihydrotestosterone. Biochem Biophys Res Commun. 2016.03; 472(1); 250-254― 170 ―

Gerontology and Gerodontology

Gerontology and Gerodontology

3. Masutomi Hirofumi, Kawashima Saki, Kondo Yoshitaka, Uchida Yoshiaki, Jang Byungki, Choi EunKyoung, Kim Yong-Sun, Shimokado Kentaro, Ishigami Akihito. Induction of peptidylarginine deiminase
2 and 3 by dibutyryl cAMP via cAMP-PKA signaling in human astrocytoma U-251MG cells. J Neurosci
Res. 2016.10;
4. Chiba Tsuyoshi, Noji Keiko, Shinozaki Shohei, Suzuki Sachina, Umegaki Keizo, Shimokado Kentaro. Dietinduced non-alcoholic fatty liver disease aﬀects expression of major cytochrome P450 genes in a mousemodel. J Pharm Pharmacol. 2016.12; 68(12); 1567-1576― 171 ―

Gerontology and Gerodontology

Gerontology and Gerodontology

Rehabilitation Medicine

Associate Professor Tetsuya JINNO
Graduate Student
Tomoko ARAKI,
Kashitarou HYOUDOU
Chisato HOSHINO
Kazuko KATSUKI
Takanori KOKUBUN
Takashi IKEDA(1)

ResearchResearch Subjects
1) Rehabilitation for total joint arthroplasty2) Three dimentional motion analysis of upper/lower extremities and gait analysis
3) Nutritional management in rehabilitation
4) Biomechanical research for prevention of sports injury(2)

Lectures & Courses

Purpose of Education:
Rehabilitation medicine consists of physical, occupational and speech therapy. Main theme of rehabilitation
medicine in graduate course is to study 3-dimentional motion analysis in activities of daily living and molecularbiological analysis of disuse atrophy.
(3)

Publications[Original Articles]
1. Takashi Ikeda, Junya Aizawa, Hiroshi Nagasawa, Ikuko Gomi, Hiroyuki Kugota, Keigo Nanjo, Tetsuya
Jinno, Tadashi Masuda, Sadao Morita. Eﬀects and feasibility of exercise therapy combined with branchedchain amino acid supplementation on muscle strengthening in frail and pre-frail elderly people requiringlong-term care: A crossover trial Applied Physiology, Nutrition, and Metabolism. 2016; 41(4); 438-445
2. Ryohei Takada, Tetsuya Jinno, Daisuke Koga, Yuki Yamauchi, Yoshinori Asou, Takeshi Muneta, Atsushi
Okawa. Limited signiﬁcance of screening computed tomography after cementless total hip arthroplasty
with highly cross-linked polyethylene at 7 to 10 years of follow-up Mod Rheumatol. 2016; 26(5); 757-760
3. Tsuyoshi Yamada, Toshitaka Yoshii, Satoru Egawa, Ryohei Takada, Takashi Hirai, Hiroyuki Inose, Tsuyoshi
Kato, Tetsuya Jinno, Atsushi Okawa. Drain tip culture is not prognostic for surgical site infection in spinal
surgery under prophylactic use of antibiotics Spine (Phila Pa 1976). 2016; 41(14); 1179-1184
4. Takanori Kokubun, Naohiko Kanemura, Kenji Murata, Hideki Moriyama, Sadao Morita, Tetsuya Jinno,
Hidetoshi Ihara, Kiyomi Takayanagi. Eﬀect of changing the joint kinematics of knees with a ruptured
anterior cruciate ligament on the molecular biological responses in a spontaneous healing in a rat model
Am J Sports Med. 2016; 44(11); 2900-2910― 172 ―

Gerontology and Gerodontology

Gerontology and Gerodontology

5. Ryohei Takada, Tetsuya Jinno, Daisuke Koga, Masanobu Hirao, Takeshi Muneta, Atsushi Okawa. Comparison of wear rate and osteolysis between annealed and remelted highly cross-linked polyethylene intotal hip arthroplasty. A 7 to 10 year follow-up Orthop Traumatol Surg Res. 2016; 102(6); 707-721[Conference Activities & Talks]
1. Gaku Koyano, Tetsuya Jinno, Daisuke Koga, Takeshi Muneta, Atsushi Okawa. Bilateral Comparison of
Hip Joint Morphology between the Hips with and without Herniation Pit. Orthopaedic Research Society
2016.03
2. Ryohei Takada, Tetsuya Jinno, Daisuke Koga, Masanobu Hirao, Takeshi Muneta, Atsushi Okawa. Comparison of wear rate and osteolysis between annealed and remelted highly cross-linked polyethylene intotal hip arthroplasty. 17th EFORT 2016.06
3. Ryohei Takada, Tetsuya Jinno, Daisuke Koga, Masanobu Hirao, Takeshi Muneta, Atsushi Okawa. Limited
signiﬁcance of screening computed tomography after cementless total hip arthroplasty with highly crosslinked polyethylene. 17th EFORT 2016.06― 173 ―

Gerontology and Gerodontology

Gerontology and Gerodontology

Gerodontology and Oral Rehabilitation

Professor
MINAKUCHI Shunsuke
Associate Professor
TOHARA Haruka, OWATARI Tsuneto
Junior Associate Professor
SEKITA Toshiaki, KOBAYASHI Ken-ichi
Assistant Professor
AKIBA Norihisa, INOKOSHI Masanao, KANAZAWA Manabu, KOMAGAMINE Yuriko, MOTOMURA Kazuo,
NAKANE Ayako, SATO Yusuke, WAKASUGI Yoko, OKUBO Mai
Project Assistant Professor
HAMA Youhei
Dental Resident
YOSHIZAKI Taro, NAKMURA Toshinarim, HIRAYAMA Daisuke, INOUE Minoru, JOU Ayami,
TANOUE Mariko, YAMAGA Eijirou, YOSHIZUMI Yuu, NOMOTO Akiko, SATO Marie, OZAWA Emi
Graduate Student
AMAGAI Noriko, ANNDOU Mariko, ARAKIDA Toshio, ASAMI Mari, BABA Yuuya, CHANTARAMANEE Ariya,
DOUKE Midori, KAGIFUKU Yuko, KAIDILIYA Yalikun, Sato Eriko, KAMOCHI Gou, KHAING Myat Thu,
KUROSAWA Yukiko, KYOSAKA Yuka, MATSUBARA Mariko, MATSUDA Yuhei, MIURA Akemi, MIYAYASU Anna,OOWADA Gaku, SHIMIZU Haruki, SHIMIZUBATA Makoto, SHINOZAKI Hiromichi, SOEDA Hitomi,
SUZUKI Hiroyuki, Shimada Ryo, TAGASHIRA Itoe, TUN Min Bo, VO Lam Thuy, YAMAGUCHI Kouhei,
YAMAZAKI Yasuhiro, YOSHIMI Kanako, YOSHINAKA Shinn, HIROKO Namai, KUBOTA Chieko, OBA Shoko
Student
HIGASHINAKAGAWA Anri, JOKO Natsuka, KAKU Jakuenn, KANEKO Seiko, KAWASHIMA Mina,
MACHIDA Nami, NAMIKI Chizuru, NISHIKI Gen, NISHIMIYA Yui, ODA Ken, OKANO Sakiko,
TAKADA Shintaro, TAMURA Atsuko, TANIMOTO Hiroyuki, UEDA Kaori, UENO Taro, Tsugawa Eriko,
YAGUCHI Shiho, YOSHII Eiji, AOKI Yumi, AWANO Mikiko, NAITO Yoko,
Staﬀ
YAJIMA Yuriko, YOSHIZAKI Ayaka
Visiting Lecturer
Ando Kazuo, Fujishima Ichiro, SANO Haruo, Hiratsuka Tomohiro, INOKUCHI Nobuhiro
FUJITANI Junko, KAGAWA Tomonori, UEMURA Ken ichi, KIKUCHI Keisuke, SATOU Koutetsu,
Kobayashi Shoji, Kojo Akiko, MURATA Shino, Matsuo Koichiro, Hino Naohiko, Nishibori Masakazu
Osada Eiji, Ozaki Ken-ichiro, SUSA Chiaki, Saito Eiichi, Satoda Takahiro, Terasaka Satoshi,
Hirano Shigezo, Kunimitsu Takashi, TAKESHITA Shin, TAKEUCHI Shuhei, TANAKA Shinji, Takahashi Noboru
Takeuchi Satoshi, Usui Yoshie, Watanabe Ikki, YAMADA Ayako, YAMAMOTO Shinta, Yoneyama Takeyoshi
Yuki Hirazima, ZHAO Hui Zi, Akimoto Kazuhiro, HAYASHI Keiji, HAYASHI Yukiko, Seki Takeo, Oono Tomohisa
YAMADA Azusa, YAMAMOTO Katsura(1)

Research

1) Medical management of Elderly Patients During Dental Treatment
2) New Examination Method for Dry Mouth
3) Oral Stereognosis Ability in the Elderly
4) Threshold of Mucous Membrane under Denture Base in Elderly Oral Mucosa Patients
5) State of the art Lasers in Zirconia Prosthetic Processing and Pain-free Treatment― 174 ―

Gerontology and Gerodontology

Gerontology and Gerodontology6) Denture Mobility7) Deglutition in Elderly Patients Requiring Nursing Care
8) Eating and Swallowing Rehabilitation in Post-Oral Tumor Surgery Patients
9) Dysphagia of Medullary Infarction Patients
10) Dental Approaches to Dysphagia11) Screening Methods of Silent Aspiration
12) Swallowing Dynamics and Brain Activity13) Stress analyses of implant overdenture14) Factorial analysis of complete denture prosthesis
15) Resilient denture lining material16) CAD/CAM system for fabricating complete dentures17) Evaluations of masticatory performance using color-changeable chewing gum(2)

Education

Given the increased health needs of an aging society, we aim to integrate diverse clinical specialties related
to geriatric dental practice and to educate individuals of fundamental studies in each ﬁeld. We emphasize acomprehensive approach to patient interactions by examining daily life functionality rather than focusing only
on their diseases.
With regard to dysphagia, which can lead to aspiration pneumonia, we provide comprehensive education on
causes, diagnostic methods, and rehabilitation options from a dentistry point of view. Since we regard rehabilitation as the medicine of daily living, we emphasize that dysphagia rehabilitation should be considered amethod to ameliorate disability rather than diseases by introducing practical approaches in addition to factual
knowledge.

(3)

Clinical Performances

We manage the prosthodontic, special care and dysphagia rehabilitation departments.

(4)

Publications[Original Articles]
1. Marie Sato. Eﬀectsof Thyroid Cartilage Skin Traction on Swallowing Function in Elderly People 2016;31(2); 100-107
2. Enri Nakayama, Haruka Tohara, Kotomi Sakai, Mayumi Hayata, Sayako Ohnishi, Sekino Jumpei, Hidetaka Tsuzuki, Koichiro Ueda. Predictive Factors associated with oral intake ability in gastrostomy patients
under long-term care Journal of Nutrition Health & Aging,. 2016;
3. Inokoshi M, Yoshihara K, Nagaoka N, Nakanishi M, De Munck J, Minakuchi S, Vanmeensel K, Zhang
F, Yoshida Y, Vleugels J, Naert I, Van Meerbeek B. Structural and Chemical Analysis of the ZirconiaVeneering Ceramic Interface. J Dent Res. 2016.01; 95(1); 102-109
4. Sato D, Kanazawa M, Kim YK, Yokoyama S, Omura Y, Ozeki M, Minakuchi S, Kasugai S, Baba K.
Immediate loading of two freestanding implants placed by computer-guided ﬂapless surgery supporting amandibular overdenture with magnetic attachments. J Prosthodont Res. 2016.01; 60(1); 54-62
5. Nami Machida, Haruka Tohara, Koji Hara, Ayano Kumakura, Yoko Wakasugi, Ayako Nakane, Shunsuke
Minakuchi. Eﬀects of aging and sarcopenia on tongue pressure and jaw-opening force. Geriatr Gerontol
Int. 2016.01;
6. Yamamoto S, Kanazawa M, Hirayama D, Nakamura T, Arakida T, Minakuchi S. In vitro evaluation ofbasal shapes and oﬀset values of artiﬁcial teeth for CAD/CAM complete dentures. Computers in Biology
and Medicine. 2016.01; 68(1); 84-89― 175 ―

Gerontology and Gerodontology

Gerontology and Gerodontology

7. Zhang F, Batuk M, Hadermann J, Manfredi G, Marien A, Vanmeensel K, Inokoshi M, Van Meerbeek B,
Naert I, Vleugels J.. Eﬀect of cation dopant radius on the hydrothermal stability of tetragonal zirconia:
Grain boundary segregation and oxygen vacancy annihilation Acta Mater. 2016.03; 106; 48-58
8. Huizi Zhao, Norihisa Akiba, Hiroyuki Tanimoto, Taro Yoshizaki, Kaidiliya Yalikun, Shunsuke Minakuchi.
Eﬀects of temperature-responsive hydrogel on viscosity of denture adhesives. Dent Mater J. 2016.04;
35(2); 210-215
9. Inokoshi M, Pongprueksa P, De Munck J, Zhang F, Vanmeensel K, Minakuchi S, Vleugels J, Naert I, Van
Meerbeek B. Inﬂuence of Light Irradiation Through Zirconia on the Degree of Conversion of Composite
Cements. J Adhes Dent. 2016.04; 18(2); 161-171
10. Uchida Yoko, Kubota Chieko. Study on the relation between LUTS and xerostomia in community residents
International Nursing Care Research. 2016.04; 15(1); 95-103
11. Eﬀect of Added Oil and Fat on the Easiness of Swallow of Modiﬁed Diet for Dysphagia Persons 2016.06;
31(1); 58-65
12. Omura Y, Kanazawa M, Sato D, Kasugai S, Minakuchi S. Comparison of patient-reported outcomes
between immediately and conventionally loaded mandibular two-implant overdentures: A preliminary
study. J Prosthodont Res. 2016.07; 60(3); 185-192
13. Komagamine Y, Kanazawa M, Sasaki Y, Sato Y, Minakuchi S. Prognoses of new complete dentures fromthe patient’s denture assessment of existing dentures. Clinical Oral Investigations. 2016.07;
14. Oda K, Kanazawa M, Takeshita S, Minakuchi S.. Inﬂuence of implant number on the movement ofmandibular implant overdentures. J Prosthet Dent.. 2016.09;
15. Sakai K, Nakayama E, Tohara H, Maeda T, Sugimoto M, Takehisa T, > Takehisa Y, Ueda K. ongue
Strength is Associated with Grip Strength and Nutritional Status in Older Adult Inpatients of a Rehabilitation Hospital. 2016.09;
16. Miura Y, Nakagami G, Yabunaka K, Tohara H, Hara K, Noguchi H, Mori T, Sanada H.. Detecting
pharyngeal post-swallow residue by ultrasound examination: a case series Med Ultrason. 2016.09; 18(3);288-293
17. Takahiro Arakawa,Yusuke Kuroki, Hiroki Nitta, Prem Chouhan, Koji Toma, Shin-ichi Sawada, Shuhei
Takeuchi, Toshiaki Sekita, Kazunari Akiyoshi, Shunsuke Minakuchi, Kohji Mitsubayashi. Mouthguard
biosensor with telemetry system for monitoring of saliva glucose: A novel cavitas sensor Biosensors andBioelectronics . 2016.10; 84; 106-111
18. Suguru Kimoto, Yasuhiko Kawai, Atsuko Gunji, Hisatomo Kondo, Taro Nomura, Tomohiko Murakami,
Akito Tsuboi, Guang Hong, Shunsuke Minakuchi, Yusuke Sato, Gaku Ohwada, Tetsuya Suzuki, Katsuhiko
Kimoto, Noriyuki Hoshi, Makiko Saita, Yoshikazu Yoneyama, Yohei Sato, Masakazu Morokuma, Joji
Okazaki, Takeshi Maeda, Kenichiro Nakai, Tetsuo Ichikawa, Kan Nagao, Keiko Fujimoto, Hiroshi Murata,
Tadafumi Kurogi, Kazuhiro Yoshida, Masahiro Nishimura, Yasuhiro Nishi, Mamoru Murakami, Toshio
Hosoi,Taizo Hamada. Study protocol for a multi-center, randomized controlled trial to develop Japanese
denture adhesive guidelines for patients with complete dentures: the Denture Adhesive Guideline trial:
study protocol for a randomized controlled trial Trials. 2016.10; 17;
19. Komagamine Y, Kanazawa M, Iwaki M, Jo A, Suzuki H, Amagai N, Minakuchi S. Combined eﬀect of newcomplete dentures and simple dietary advice on nutritional status in edentulous patients: study protocol
for a randomized controlled trial. Trials. 2016.11; 17(1); 539-546
20. Zhang F, Inokoshi M, Batuk M, Hadermann J, Naert I, Van Meerbeek B, Vleugels J. Strength, toughness
and aging stability of highly-translucent Y-TZP ceramics for dental restorations. Dent Mater. 2016.12;32(12); e327-e337
21. Mariko Tanoue, Manabu Kanazawa, Shin Takeshita, Shunsuke Minakuchi. Eﬀects of clip materials onstress distribution to maxillary implant overdentures with bar attachments Journal of Prosthetic Dentistry.
22. Khaing Myat Thu, Khin Swe Aye, Aung Htang. In vitro study of Coronal Leakage of Four Temporary
Filling Materials Immersed in Alcoholic Methylene Blue Dye Myanmar Dental Journal . 20(1); 49-57― 176 ―

Gerontology and Gerodontology

Gerontology and Gerodontology

23. Yu Yoshizumi,Shinya Mikushi,Ayako Nakane,Haruka Tohara,Shunsuke Minakuchi. Dynamics of Swallowing Tablets during the Recovery Period following Surgery for Tongue Cancer Otolaryngology.
[Books etc]
1. Shinya Mikushi, Haruka Tohara. Anatomy for Plastic Surgery of the Face, Head and Neck. 2016
[Misc]
1. Manabu Kanazawa, Maiko Iwaki, Shinta Yamamoto, Toshio Arakida and Shunsuke Minakuchi. The
complete denture with using CAD/CAM system 2016.06; 35(3); 185-188
2. Inokoshi M, Minakuchi S. Bond strategy for zirconia ceramics The Journal of the Japanese Society for
Dental Materials and Devices. 2016.07; 35(4-5); 265-268[Conference Activities & Talks]
1. Jo A,Kanazawa M,Sato Y,Akiba N,Iwaki M, Komagamine Y, Minakuchi S. Inﬂuence of the diﬀerent
impression methods for complete denture fabrication on masticatory ability. 2016.01.24
2. Manabu Kanazawa. Live border molding for complete dentures. Essential Expertise for Clinical Dentistry
2 2016.02.09
3. Manabu Kanazawa. Mandibular 2-implant overdenture. Essential expertise for clinical density 2016.02.26
4. Yuka Kyosaka,Shusaku Tayama,Tsuneto Owatari . A case report of prilocaine-induced methemoglobinemia in a medically compromised elderly. 2016.03.06
5. Arakida T,Kanazawa M,Yamamoto S,Iwaki M, Minakuchi S,Suzuki T. The eﬀect if external light of digital
impression. The eﬀect of external light of digital impression 2016.05.29
6. Inokoshi M, Zhang F, De Munck J, Vanmeensel K, Minakuchi S, Vleugels J, Naert I and Van Meerbeek
B. Inﬂuence of surface treatment on the bending strength of zirconia. 94th General Session & Exhibitionof the IADR 2016.06 Seoul, Korea
7. M. Kanazawa, M. Iwaki, D. Sato, Y. Omura, A. Miyayasu, S. Kasugai, S. Minakuchi.. Immediate Loadingof two-implants mandibular overdentures: 4-years prospective study.. 94th General Session & Exhibitionof the IADR 2016.06 Seoul, Republic of Korea
8. Y. Komagamine, M. Kanazawa, H. Suzuki, N. Amagai, S. Minakuchi.. Eﬀect of New Removable Dentures
on Foods and Nutrients Intakes . 94th General Session & Exhibition of the IADR 2016.06 Seoul, Republicof Korea
9. Yuka Kyosaka,Tsuneto Owatari,Kazuki Takahashi,Natsuka Joko,Ayano Hayashi,Kazuhiro Shimoyama,Shunsuke
Minakuchi. A case report of drug-induced arrhythmia : Excessive use of tiotropium in a medically compromised elderly in dental treatment.. 2016.06.18
10. Eriko Kajisa, Haruka Tohara, Ayako Nakane, Atsuko Tamura, Kohei Yamaguchi, Kanako Yoshimi, Mina
Kawashima, Mikiko Awano, Shunsuke Minakuchi. The Relationship between Jaw Opening Force andSkeletal Muscle Mass including Suprahyoid Muscles. 2016.06.18
11. Hiromichi Shinozaki, Haruka Tohara, Mariko Matsubara, Yasuhiro Yamazaki, Koji Hara, Ayako Nakane,
Yoko Wakasugi, Shunsuke Minakuchi. Eﬀect of Decreased Jaw Opening Force on Hyoid bone Movement
and its Resting Position in Healthy Elderly Subjects. 2016.06.18 Asty Tokushima
12. Minoru Inoue, Tsuneto Owatari, Hiroyuki Suzuki, Seiko Kaneko, Makoto Shimizubata, Noriko Amagai,
Syunsuke Minakuchi, Kazuhiro Shimoyama. A case report of the urgent hospitalization of an elderly
patient due to the actualized lung infection after the tooth extraction. 2016.06.19
13. Kanako Yoshimi,Haruka Tohara,Eriko Kajisa,Kohei Yamaguchi,Mikiko Awano,Mina Kawashima,Yoko
Wakasugi,Ayako Nakane,Shunsuke Minakuchi. The Relationship between Oral Function and Leisure Activity in Community-dwelling Elderly People. 2016.06.19― 177 ―

Gerontology and Gerodontology

Gerontology and Gerodontology

14. M. YAMAMOTO, K. MOTOMURA, H. NAKAMURA, T. YOSHIOKA, F. YOSHIKAWA, S. TANAKA.
Optimum conditions for resistance-heat-bending of NiTi arch wire. 94rd General Session & Exhibition of
the IADR, 3rd Meeting of the Asia Paciﬁc Region, 35th Annual Meeting of the IADR Korean Division
2016.06.23 Seoul
15. F.YOSHIKAWA, K.MOTOMURA, H.NAKAMURA, T.YOSHIOKA, M.YAMAMOTO, S.TANAKA. Improving adhesion of zirconia by surface modiﬁcation. 94rd General Session & Exhibition of the IADR,
3rd Meeting of the Asia Paciﬁc Region, 35th Annual Meeting of the IADR Korean Division 2016.06.24
Seoul
16. Anna Miyayasu, Ayami Jo, Manabu Kanazawa, Yusuke Sato, Maiko Iwaki, Norihisa Akiba, Shunsuke
Minakuchi. Cost Analysis of the diﬀerent impression methods for complete dentures. 94th General
Session & Exhibition of the IADR 2016.06.25 Seoul, Korea
17. Y Kaidiliya, M Kanazawa, S Takeshita, K Oda ,M Tanoue, A Miyayasu, V L Thuy, K M Thu, M Asami,
H Shimizu, S Minakuchi. Stress Analysis of Mandibular Implant Overdentures with Diﬀerent Implant
Positions. 94th General Session & Exhibition of the IADR 2016.06.25 Seoul, Korea
18. Hama Y, Minakuchi S, Sasaki K, Maeda T, Hamura A, Ichinohe T, Keiji M. The Dental Education
Consortium to promote healthy longevity -Second report- Agendas after the second year. 2016.07.02
19. Multicenter Randomized Clinical Study of Denture Adhesive to Establish the Guideline: Study by the
Subgroup Analysis for a Denture Adhesive on the Eﬀect of the Masticatory Performance. 2016.07.09
20. Arakida T,Kanazawa M,Yamamoto S,Iwaki M, Minakuchi S,Suzuki T,Oki M,Kamijo S,Ando K,Morisawa
K. The eﬀect of external light of digital impression time and accuracy. 2016.07.09
21. Miyayasu A, Jo A, Kanazawa M, Sato Y, Iwaki M, Akiba N, Minakuchi S. Cost-eﬀectiveness analysisof the impression methods for mandibular complete dentures in randomized controlled trial. The 125th
Annual Meeting of the Japan Prosthodontic Society 2016.07.09 Kanazawa
22. Hiroyuki Suzuki,Manabu Kanazawa,Yuriko Komagamine,Maiko Iwaki,Ayami Jo,Noriko Amagai,Shunsuke
Minakuchi. Combined eﬀect of new complete dentures and simple dietary counseling on nutritional statusin edentulous patients : study protocol for a randomized controlled trial. The 125th Scientiﬁc Meeting ofJapan Prosthodontic Society 2016.07.09 Kanazawa
23. Asami Mari,Miyayasu Anna,Kanazawa Manabu,Takeshita Shin,Tanoue Mariko,Sato Daisuke,Oda Ken,Shimizu
Haruki,Morisawa Masayuki,Kasugai Shohei,Minakuchi Shunsuke. Cost-eﬀectiveness analysis of mandibular single-implant overdentures and conventional complete dentures Study protocol for a randomized controlled clinical trial . The 125th Scientiﬁc Meeting of Japan Prosthodontic Society 2016.07.09 Kanazawa
24. Kobayashi K, Sekita T, Kobayashi K, Hoshino T, Takeuchi S. A 25-year Follow-up of the Patient with
Advanced Periodontal Disease Treated by Cross-arch Fixed Bridge. 2016.07.09
25. Kobayashi K, Sekita T, Kobayashi K, Hoshino T, Takeuchi S. A 25-year Follow-up of the Patient with
Advanced Periodontal Disease Treated by Cross-arch Fixed Bridge. The 125rd Annual Meeting of Japan
Prothodontic Society 2016.07.09 Kanazawa
26. Zhang F, Vanmeensel K, Inokoshi M, Van Meerbeek B, Naert I, Vleugels J.. Towards aging resistantzirconia: Grain boundary engineering. International Congress on Ceramics (ICC6) 2016.08 Dresden
27. Yoko Wakasugi, Toshiyuki Yamamoto, Miho Murata, Haruka Tohara, Syunsuke Minakuchi. Eﬀect of an
impaired oral stage of swallowing in patients with Parkinson’s disease. 2016.09.23
28. Y. Komagamine, M. Kanazawa. N. Akiba, Y. Sato, M. Okubo, Y. Hama, S. Minakuchi. The comparisonof the nutritional status between pre- and post- provision with new complete denture among edentulous
people. The 23rd General Meeting of the Japanese Association for Dental Science 2016.10.21 Fukuoka
Convention Center, Hakata, Fukuoka
29. Anna Miyayasu, Manabu Kanazawa, Yuriko Komagamine, Naoko Seki. Decision making for prosthodontics treatment in a case of multiple tooth loss. Essential Expertise for Clinical Dentistry 2 2016.10.27
Tokyo, Japan
30. Inokoshi M. Basics of dental zirconia. Tokyo Meister Course Lecture 2016 2016.10.30 Tokyo― 178 ―

Gerontology and Gerodontology

Gerontology and Gerodontology

31. Kaidiliya Yalikun, Manabu Kanazawa, Shin Takeshita, Ken Oda ,Mariko Tanoue, Anna Miyayasu, Vo
Lam Thuy, Khaing Myat Thu, Mari Asami, Haruki Shimizu, Ryo Shimada, Shunsuke Minakuchi. Strain
Analysis of Mandibular Implant Overdentures in Diﬀerent Dislodging Position with Diﬀerent Implant
Number.. International Dental Materials Congress 2016 (IDMC2016) 2016.11 Bali, Indonesia
32. Shimada Ryo, Kanazawa Manabu, Miyayasu Annna, Tanoue Mariko, Sato Daisuke, Kusumoto Yuriko,
Abe Yuka, Yokowama Sawako, Baba Kazemi, Minakuchi Syunnsuke. Prospective clinical trial of IARPD
with short implants -research protocol- . 2016.11.06 gihu
33. Go KAMOCHI, Norihisa AKIBA, Toshinari NAKAMURA, Shunsuke MINAKUCHI. Eﬀect of water absorption on stress relaxation behavior of home reliner. 2016.11.26[Others]
1. Kobayashi K, Principle of tooth preparation, 2016.04
Hands-oncourse
2. Kobayashi K, Principle of tooth preparation, 2016.10
Hands-on course
3. Kobayashi K, Principle of tooth preparation for molar teeth, 2016.11
Hands-on course― 179 ―

Comprehensive Patient Care

Comprehensive Patient Care

Laboratory Medicine

Professor Shuji TOHDA
Research Associate Mai ITOH
Graduate Students Mika OHTAKA, Erika SHIRATORI, Shohei NOGAMI, Yuki KOHDA(1)

OutlineLaboratory medicine is a ﬁeld of research to develop analytical methods of pathophysiology of various diseases,
new diagnostic tests, and diagnosis-supporting system using laboratory tests.
(2)

Research

Our research subjects are as follows:
1) Cellular and molecular mechanism of abnormal growth of acute leukemia cells and drug-sensitivity tests formolecularly targeted thepapy
2) Molecular diagnostic tests for cancer and detection of minimal residual cancer cells3) Mechanism of abnormal growth of lymphoma cells4) Molecular diagnostic tests for infectious diseases(3)

Education

To graduates students, we provide opportunity to study and develop novel diagnostic tests using cellular andmolecular biological techeque in our laboratories.
To undergraduate students, we give a lecture and practical training on laboratory medicine.

(4)

Lectures & Courses

Main objective of Laboratory Medicine in the graduate course is to provide students opportunity to study
analysis of pathophysiology, development of new diagnostic tests, and establishment of diagnosis-supporting
system using laboratory tests. We focus on the analysis of pathophysiology of hematological malignancies andthe development of molecular diagnostic tests for cancer and infectious diseases.
(5)

Clinical Services & Other Works

We are performing laboratory tests for hematology, clinical chemistry, immunology, and microbiology in cooperation with doctors and technologists of clinical laboratory in University hospital. We give a lecture onlaboratory tests at meetings of laboratory medicine-related societies.
(6)

Clinical Performances

We are developing new diagnostic methods collaborating with various clinical departments. We are also supporting them in their diagnostic procedure.

― 180 ―

Comprehensive Patient Care

Comprehensive Patient Care
(7)

Publications[Original Articles]
1. Mari Kamiya, Peter Y Shane, Makoto Soejima, Shuji Tohda, Nobuyuki Miyasaka, Hitoshi Kohsaka.
IgG4-Related Sialoadenitis with a Skin Lesion and Multiple Mononeuropathies Suggesting Coexistent
Cryoglobulinemic Vasculitis. Intern. Med.. 2016; 55(10); 1355-1361
2. Megumi Akiyama, Kota Yoshifuji, Tetsuya Fukuda, Shuji Tohda, Tohru Miki, Osamu Miura, Masahide
Yamamoto. Fulminant visceral disseminated varicella-zoster virus infection without skin involvement ina patient with autoimmune hemolytic anemia on prednisolone therapy. Rinsho Ketsueki. 2016.04; 57(4);467-471
3. Noriko Kawaguchi-Ihara, Mai Itoh, Ikuo Murohashi, Shuji Tohda. Establishment of a quenching probe
method for detection of NPM1 mutations in acute myeloid leukemia cells. Oncol Lett. 2016.04; 11(4);2429-2432
4. Hiroko Nagata, Mina Nakagawa, Yuki Nishimura-Sakurai, Yu Asano, Tomoyuki Tsunoda, Masato Miyoshi,
Shun Kaneko, Fumio Goto, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako Murakawa, Sayuri Nitta,
Yasuhiro Itsui, Seishin Azuma, Sei Kakinuma, Naoko Tojo, Shuji Tohda, Yasuhiro Asahina, Mamoru
Watanabe, . Serial measurement of Wisteria ﬂoribunda agglutinin positive Mac-2-binding protein is
useful for predicting liver ﬁbrosis and the development of hepatocellular carcinoma in chronic hepatitis C
patients treated with IFN-based and IFN-free therapy.[ Epub ahead of print] Hepatol Int. 2016.07;
5. Shinji Ogihara, Ryoichi Saito, Etsuko Sawabe, Michio Hagihara, Shuji Tohda. First Japanese case of
infectious endocarditis due to Enterococcus faecalis small-colony variants. J. Infect. Chemother.. 2016.10;
22(10); 716-719
6. Gene Igawa, Mika Casey, Etsuko Sawabe, Yoko Nukui, Shu Okugawa, Kyoji Moriya, Ryuji Koike, Shuji
Tohda, Ryoichi Saito. Comparison of agar dilution and broth microdilution methods for Clostridium
diﬃcile antimicrobial susceptibility testing. J Glob Antimicrob Resist. 2016.12; 7; 43-45[Conference Activities & Talks]
1. Amano E, Suzuki M, Ozaki K, Ishibashi S, Akaza M, Kanouchi T, Tohda S, Yokota T. A case of sensory
dominant CIDP with conduction delay predominantly in the most distal segment (Japanese). 10th Tokyo
Metropolitan Neuromuscular Electrodiagnosis Forum 2016.01.30 Tokyo
2. Shohei Nogami, Mai Itoh, Shuji Tohda. Clinical usefulness of allele-speciﬁc/quenching probe method for
detection of the MYD88 L265P mutation. The 14th Asian Society for Clinical Pathology and Laboratory
Medicine Congress 2016.03.26 Taipei
3. Ohta N, Kanouchi T, Akaza M, Aoyagi E, Narumi J, Yanagi N, Sumi Y, Yokota T, Hagihara M, Tohda
S. A study of quality control of nerve conduction study. 57th annual meeting of the Japanese Society of
Neurology 2016.05.20 Kobe
4. Kazuaki Yamamoto, Seiya Watanabe, Michio Hagihara, Shuji Tohda. Development of a novel measurement method for L-hydroxyproline using a new enzyme. The 32th World Congress of Biomedical
Laboratory Science 2016.09.02
5. Naoya Ichimura, Ayako Itoi, Yuki Ohkubo, Yuki Koda, Michio Hagihara, Shuji Tohda. Development of
an internal quality control application for immature granulocytes diﬀerential. The 32th World Congressof Biomedical Laboratory Science 2016.09.02 Kobe
6. Naoya Ichimura, Ayako Itoi, Yuki Ohkubo, Yuki Koda, Michio Hagihara, Shuji Tohda. Evaluation of aninternal QC application for correctly recognizing immature granulocytes on blood smear. The 32th World
Congress of Biomedical Laboratory Science 2016.09.02 Kobe
7. Kanouchi T, Sekiguchi T, Tohda S, Yokota T. Does the impairment of upper motor neurons have aninﬂuence upon the speed of degeneration of lower motor neurons in ALS. 46th annual meeting of theJapanese Society of Clinical Neurophysiology 2016.10.28 Kohriyama― 181 ―

Comprehensive Patient Care

Comprehensive Patient Care

8. Aoyagi E, Kanouchi T, Akaza M, Iida S, Narumi J, Ohta N, Sumi Y, Yokota T, Hagihara M, Tohda S.
A study of quality control of nerve conduction study. 46th annual meeting of the Japanese Society ofClinical Neurophysiology 2016.10.29 Kohriyama
9. Erika Shiratori, Mai Itoh, Mika Ohtaka, Shohei Nogami, Shuji Tohda. Mechanisms of suppressive eﬀects
of MYD88 Inhibitors on the Growth of Lymphoma and Leukemia Cells. The 58th American Society of
Hematology 2016.12.04 San Diego― 182 ―

Comprehensive
Comprehensive Patient
Patient Care
CareIntensive Care Medicine

Professor:
Hidenobu Shigemitsu (2016.9.1 -)Associate Professor
Hideo Takahashi(2016.4.1 -)Yushi Adachi(2015.4.1 - 2016.6.30)
Assistant Professor:
Takahiro Masuda (Intensive Care Unit) (2014.4.1 -)Kenji Wakabayashi (2015.4.1 -)Akihiro Haramo (Intensive Care Unit) (2015.4.1 - 2017.3.31)
Nobuhiro Shiota (Intensive Care Unit) (2015.4.1 -)Arisa Fukagawa (Intensive Care Unit) (2016.4.1 - 2017.3.31)
Kanae Ochiai (Intensive Care Unit) (2016.7.1 - 2017.1.31)
Takafumi Ohmori (Anesthesiology) (2016.4.1 - 2017.3.31)
Fellow:
Fumi Maruyama (Intensive Care Unit) (2014.4.1 -)Postgraduate students:
Mariko Senda (2014.4.1 -)Yusuke Mitsui (2016.4.1 -)Shotaro Matsumoto (2016.4.1 -)(1)OutlineCritical care medicine provides intensive care and treatment for critically ill patients. To treat critically illpatients, intensivists have to catch the changes of the patients condition by monitoring and evaluation, andpractice appropriate therapy. It is important that intensivists practice minute-to-minute titration therapy incooperation with other multidisciplinary professionals.
Practice of critical care medicine includes intensive care for various types of shock, acute respiratory distresssyndrome/acute lung injury, sepsis, multiple organ dysfunction syndrome, abnormal acid-base balance, electrolyte disturbance, acute kidney injury, central nervous system dysfunction, and hospital-acquired infection,mechanical ventilation, pharmacological support, cardiopulmonary support system, blood puriﬁcation, and nutrition support.

(2)

Research

Our themes of research are derived from clinical questions in critically ill patients.
Clinical research:
1) Conducted Multiple institutes randomized control trial: Japanese Trial for Acute Kidney Injury in Postcardiovascular Surgery by Atrial Natriuretic Peptide (JAPAN study).

― 183 ―

Comprehensive Patient
Patient Care
Care
Comprehensive2) Participated multiple center clinical investigation: Mechanical ventilation practice investigation (MVP investigation).
3) Participated multiple center randomized control trial: Acid-base Balance & Oxygen management in patientsrequired mechancial Ventilation - Evaluation study (ABOVE study).
Eleven Japanese hospitals were included in this trial.
Basic research:
1) Eﬀect of inhaled insulin on acute lung injury (Nakazawa, Funded by Grant-in-Aid for challenging Exploratory
Research)
2) Interaction between diﬀefent anaesthetics (Adachi, funded by Grand-in-Aid for Scientiric Research C)3) Mechanism of inﬂammation in acute lung injury (Wakabayashi, funded by Grant-in-Aid for Young Scientists
B)4) Role of microvesicles in bronchopulmonary dysplasia (Wakabayashi, funded by Grant-in-Aid for Young Scientists B)
5) Role of urinary microvesicles in acute liver failure (Wakabayashi, funded by National Center of Child Healthand Development)(3)

Education

Undergraduate education
Lectures: Fourth-year medical students
1) Mechanical ventilation (Wakabayashi)
Clinical clerkship: Fifth-year and Sixth-year medical students
Critical care medicine is a branch of faculty of medicine which deals with monitoring and care of critically illpatients. Main objective of critical care medicine is to provide students opportunity to study diagnosis andtreatment of critically ill patients in the intensive care unit (ICU). Students are taught on clinical practice in
the ICU. Students take charge of 1-2 patients with attending physician and intensivist. Students check clinicaldata every morning and evening and make system-oriented presentation at ICU rounds.
Conference: Students are assigned to read recent articles of critical care medicine and make presentations by
power point at the conference.
Residents: Residents are training in the ICU for 2-3 months. They study respiratory, circulatory, and metabolic
management of critically ill patients. They learn how to use ultrasound and bronchoscope.
(4)

Clinical Services & Other WorksIntensivists are staying in the ICU, and take charge treatment of critically ill patients in the ICU.
Every morning, intensivists, nurses, pharmarcist, nutritionist, rehabilitation staﬀs, infection control staﬀs, medical engineers, and attending physicians get together, go round, and talk about the best treatment of the patients.

(5)

Publications[Original Articles]
1. Yukako Obata, Yushi U Adachi, Katsumi Suzuki, Taiga Itagaki, Hiromi Kato, Maiko Satomoto, Yoshiki
Nakajima. The Inﬂuence of Diﬀerences in Solvents and Concentration on the Eﬃcacy of Propofol at
Induction of Anesthesia. Anesthesiol Res Pract. 2016.01; 2016; 9178523
2. Chieko Mitaka, Takahiro Masuda, Koji Kido, Tokujiro Uchida, Shinya Abe, Taku Miyasho, Makoto
Tomita, Eiichi Inada. Polymyxin B hemoperfusion prevents acute kidney injury in sepsis model. J. Surg.
Res.. 2016.03; 201(1); 59-68
3. Z Sun, M Satomoto, Y U Adachi, H Kinoshita, K Makita. Inhibiting NADPH oxidase protects against
long-term memory impairment induced by neonatal sevoﬂurane exposure in mice. Br J Anaesth. 2016.05;
117(1); 80-86
4. Takahiro Masuda, Koichi Nakazawa, Chieko Mitaka. A case of atypical hemolytic uremic syndrome
triggered by a surgery, and treated with eculizumab. J Jpn Soc Intensive Care Med. 2016.11; 23(6);673-674― 184 ―

Comprehensive
Comprehensive Patient
Patient Care
Care

[Misc]
1. Koichi Nakazawa, Ken Shinoda. An overview of incidence and outcome of perioperative pulmmonary
aspiration The Japanese Journal of Anesthesiology . 2016.01; 65(1); 4-12
[Conference Activities & Talks]
1. Wakabayashi K, Adachi YU, Tateishi T, Inase N, Nakazawa K. Ventilator management for the patientwith acute exacerbation of interstitial pneumonia. Japanese Society of Intensive Care Society 2016.02
2. Eﬀects of non-invasive positive airway pressures on right internal jugular vein size. . 2016.05.30
3. Kenji Wakabayashi. Biotrauma and multiple organ dysfunction syndrome. The 38th annual congress ofJapanese Society of Respiratory Care Medicine 2016.07 Nagoya, Japan― 185 ―

Comprehensive Patient Care

Comprehensive Patient Care

Liaison Psychiatry and Palliative Medicine

Associate Professor Eisuke MATSUSHIMA
Assistant Professor Miho MIYAJIMA
Visiting Lecture Tetsuya Matsuda,Katsuya Ota,
Kanako Ichikura,Takashi Hosaka,
Clinical Psychologist Aya Yamashita,Nao Nakayama,
Graduate Student Noriko Ishiduka,Miho Itou,
Toshi Kuriyama,Hiroshi Koubou,
Toshimi Takano,Rie Tani,
Takamasa Noda,Noriko Yoshida,
Hiroko Arioka,Nami Kondou,
Hiroki Sakurai,Mariko Takaki,
Sumie Nemoto,Reiko Matsuda,
Kisaragi Suzuki,Yoko Suzuki,
Jun Nakagawa,Mayo Fujiwara,
Kanako Amano,Sayaka Ozaka,
Saori Koshimoto,Shiho Matsuoka,
Kazuhiro Kosugi,Takafumi Watanabe,
Mayuko Iijima,Yu Okura,Jun Kakou,
Kensuke Komatsu,
Research Student Okihiko Aihara, Ryuho Ibaraki.
Secretary Yoriko Mizukane(1)

Outline

The purpose of the section is to help understanding characteristics of psychosocial distress in patients with physical and mental disorders from a comprehensive viewpoint. Objects are mainly physical patients accompaniedwith pain, anxiety, depressive mood and so on. Students study these patients symptoms, how to diagnose,
practice of treatment and methods of preventive measures.
(2)

Research

1) Assessment of mental state in cancer and other physical patients using written questionnaire
2) Research on quality of life (QOL) in cancer patients and their families
3) Investigation cognitive function of patients with organic disorders (SLE, diabetics, and so on) undergoing abattery of psychometry tests and neuroimaging examinations
4) Explanation for the relationship between physical symptoms and mental states in patients with psychosomatic diseases including chronic pain and irritable bowel syndrome (IBS)
5) Examination for physiological phenomenon of psychiatric patients using eye mark recorder, electroencephalogram (EEG) and functional MRI (fMRI)― 186 ―

Comprehensive Patient Care

Comprehensive Patient Care

(3)

Clinical Services & Other Works

Psychosomatic clinic provides consultation-liaison psychiatry services at the request of the treating medical or
surgical staﬀs. Patients accompanied with insomnia, anxiety, depressive mood and delirium are treated withpsychotherapy and prescription medicines.
(4)

Publications[Original Articles]
1. Fujiwara K,Miyajima M,Yamakawa T,Abe E,Suzuki Y,Sawada Y,Kano M,Maehara T,Ota K,Sasai-Sakuma
T,Sasano T,Matsuura M,Matsushima E. Epileptic seizure prediction based on multivariate statistical process control of heart rate variability features IEEE Trans Biomed Enq. 2016; 63(6); 1321-1332
2. Kanako Ichikura, Aya Yamashita, Taro Sugimoto, Seiji Kishimoto, Eisuke Matsushima. Persistence of
psychological distress and correlated factors among patients with head and neck cancer. Palliat Support
Care. 2016.02; 14(1); 42-51
3. Shiho Matsuoka, Naoko Kato, Takahiro Kayane, Michiyo Yamada, Masako Koizumi, Toshimi Ikegame,
Miyuki Tsuchihashi-Makaya. Development and Validation of a Heart Failure-Speciﬁc Health Literacy
Scale. J Cardiovasc Nurs. 2016.03; 31(2); 131-139
4. Jun Hamano, Takashi Yamaguchi, Isseki Maeda, Akihiko Suga, Takayuki Hisanaga, Tatsuhiko Ishihara,
Tomoyuki Iwashita, Keisuke Kaneishi, Shohei Kawagoe, Toshiyuki Kuriyama, Takashi Maeda, IchiroMori, Nobuhisa Nakajima, Tomohiro Nishi, Hiroki Sakurai, Satofumi Shimoyama, Takuya Shinjo, Hiroto
Shirayama, Takeshi Yamada, Tatsuya Morita. Multicenter cohort study on the survival time of cancer
patients dying at home or in a hospital: Does place matter? Cancer. 2016.05; 122(9); 1453-1460
5. Koichi Fujiwara, Miho Miyajima, Toshitaka Yamakawa, Erika Abe, Yoko Suzuki, Yuriko Sawada, Manabu
Kano, Taketoshi Maehara, Katsuya Ohta, Taeko Sasai-Sakuma, Tetsuo Sasano, Masato Matsuura, Eisuke
Matsushima. Epileptic Seizure Prediction Based on Multivariate Statistical Process Control of Heart Rate
Variability Features. IEEE Trans Biomed Eng. 2016.06; 63(6); 1321-1332
6. Naoki Matsuo, Tatsuya Morita, Yoshinobu Matsuda, Kenichiro Okamoto, Yoshihisa Matsumoto, Keisuke
Kaneishi, Takuya Odagiri, Hiroki Sakurai, Hideki Katayama, Ichiro Mori, Hirohide Yamada, Hiroaki
Watanabe, Taro Yokoyama, Takashi Yamaguchi, Tomohiro Nishi, Akemi Shirado, Shuji Hiramoto, Toshio
Watanabe, Hiroyuki Kohara, Satofumi Shimoyama, Etsuko Aruga, Mika Baba, Koki Sumita, Satoru Iwase.
Predictors of Responses to Corticosteroids for Cancer-Related Fatigue in Advanced Cancer Patients: A
Multicenter, Prospective, Observational Study. J Pain Symptom Manage. 2016.07; 52(1); 64-72
7. Kanako Ichikura, Yasuyuki Okumura, Takashi Takeuchi. Associations of Adverse Clinical Course andIngested Substances among Patients with Deliberate Drug Poisoning: A Cohort Study from an Intensive
Care Unit in Japan. PLoS ONE. 2016.08; 11(8); e0161996
8. Miho Ito, Eisuke Matsushima. Presentation of Coping Strategies Associated with Physical and Mental
Health During Health Check-ups. Community Ment Health J. 2016.08; 53(3); 297-305
9. Ando K, Soshi T, Nakazawa K, Noda T, Okada T. Risk Factors for Problematic Behaviors among Forensic
Outpatients under the Medical Treatment and Supervision Act in Japan. Front Psychiatry. 2016.08; 7;
144
10. Shiho Matsuoka, Miyuki Tsuchihashi-Makaya, Akiko Okada, Yoshiyuki Aoki, Mariko Fukawa, Yoko Ishida,
Etsuko Nakatsugawa, Debra K. Moser. . Japanese Version of the Control Attitude Scale-Revised is Validand Reliable in Patients With Heart Failure J Card Fail. 2016.08; 22(8); s78
11. Shiho Matsuoka, Miyuki Tsuchihashi-Makaya, Takahiro Kayane, Michiyo Yamada, Rumi Wakabayashi,
Naoko P Kato, Miyuki Yazawa. Health literacy is independently associated with self-care behavior inpatients with heart failure. Patient Educ Couns. 2016.09; 99(6); 1026-1032― 187 ―

Comprehensive Patient Care

Comprehensive Patient Care

12. Shiho Matsuoka, Akiko Hayashi, Miyuki Tsuchihashi-Makaya. Daily Physical Symptom Burden in Patients with Heart Failure: A Systematic Review. J Card Fail. 2016.09; 22(9); s210
13. Midori Ninomiya-Baba, Junko Matsuo, Daimei Sasayama, Hiroaki Hori, Toshiya Teraishi, Miho Ota,
Kotaro Hattori, Takamasa Noda, Ikki Ishida, Shigenobu Shibata, Hiroshi Kunugi. Association of bodymass index-related single nucleotide polymorphisms with psychiatric disease and memory performance ina Japanese population. Acta Neuropsychiatr. 2016.12; 1-10
14. Go Taniguchi, Miho Miyajima, Masako Watanabe, Yoshiko Murata, Daichi Sone, Yutaka Watanabe,
Mitsutoshi Okazaki, Motonori Kobayashi-Kimura, Masaaki Kato, Teiichi Onuma. . Nonconvulsive statusepilepticus in the elderly associated with newer antidepressants used at therapeutic doses: A report ofthree cases. Epilepsy Behav. (accepted).
[Conference Activities & Talks]
1. Jun Kako,Masamitsu Kobayashi,Yusuke Kanno,Asao Ogawa. Establishing a binary cut-oﬀ point for thephysical scores of edmonton symptom assessment system-revised Japanese version. The MASCC/ISOO
2016 Annual Meeting 2016.06.23
2. Jun Kako, Masamitsu Kobayashi, Yusuke Kanno, Keita Tagami, Chiemi Saito. Administration of
intranasal vinegar was eﬀective for persistent hiccups with an advanced cancer patient in palliative caresetting a case report. The MASCC/ISOO 2016 Annual Meeting 2016.06.23
3. Masamitsu Kobayashi, Jun Kako, Yusuke Kanno, Asao Ogawa. Evaluation of physical symptoms using
the edmonton symptom assessment system-revised Japanese version and binary data. The MASCC/ISOO
2016 Annual Meeting 2016.06.23
4. Sasaki A, Tomita N, Kawashima I, Nishi Y, Nomura M, Setoyama S, Noda T, Kumano H. Eﬀectivenessand mechanism of Attention Training Technique on depressed patients by observing two cases.. 8thWorld Congress of Behavioural and Cognitive Therapies 2016.06.24 Australia (Melbourne Convention
and Exhibition Centre)
5. Kanako Ichikura,Shin-ichi Suzuki. Experience-oriented learning vs. lecture-based learning about cognitive
behavioral therapy for lifestyle modiﬁcation: a pilot study. 8th World Congress of Behavioural andCognitive Therapies 2016.06.25 Melbourne, Australia
6. A randomized controlled trial evaluating the eﬀect of workbook intervention on QOL in patients receiving
chemotherapy. . 2016.07.28
7. Miho Miyajima. Real-Time Epileptic Seizure Prediction System Employing a Wearable HRV Telemeterand a Smartphone. 38TH Annual International Conference of The IEEE Engineering in Medicine andBiology Society 2016.08.19 Orlando
8. Changes of heart rate and heart rate variability in the patients who found prolonged seizure during
electroconvulsive therapy. 2016.10
9. Changes of corrected QT interval during electroconvulsive therapy relate to the risk of ventricular arrhythmias. 2016.10
10. Kanako Amano,Osamu Takahashi. Psychological issues and interventions for young partners in palliative
care ward-How can we support young partners in short term?-. 18th World Congress International Psychooncology Society 2016.10.18 Dublin/Ireland― 188 ―

Comprehensive
Comprehensive Patient
Patient Care
Care

Pharmacokinetics and Pharmacodynamics

Professor Masato Yasuhara
Associate Professor Masashi Nagata
Research student Yuri Kimura
Secretary Takako Iguchi(1)

Research

1) Kinetics of drug action in disease states
2) Therapeutic drug monitoring and clinical pharmacokinetics(2)

Education

Department of Pharmacokinetics and Pharmacodynamics is in charge of the education of pharmacokinetics andpharmacodynamics for the establishment of safe and eﬀective drug therapy. In the graduate course, the lectureon the recent progress of the pharmacokinetic analysis and drug transport will be given. Students will have thepractice of pharmacokinetic analysis and animal experiments.
(3)

Publications[Original Articles]
1. Yuki Aoki, Reiji Miyawaki, Kohsuke Imai, Masatoshi Takagi, Michiko Kajiwara, Yasuyoshi Ishiwata,
Masato Yasuhara, Tomohiro Morio, Shuki Mizutani, Daisuke Tomizawa. Haploidentical Bone Marrow
Transplantation With Clofarabine and Busulfan Conditioning for a Child With Multiple Recurrent Acute
Lymphoblastic Leukemia. J. Pediatr. Hematol. Oncol.. 2016.01; 38(1); 39-41
2. M. Nagata, M. Nakajima, Y. Ishiwata, Y. Takahashi, H. Takahashi, K. Negishi and M. Yasuhara. Mechanism underlying induction of hyperglycemia in rats by single administration of olanzapine. Biol. Pharm.
Bull.. 2016.05; 39(5); 754-761
3. Yasuyoshi Ishiwata, Masashi Nagata, Takafumi Arai, Misato Makiishi, Maho Yoshikawa, Hiromitsu Takahashi, Hitoshi Kohsaka, Masato Yasuhara. Eﬀects of Miconazole Oral Gel on Blood Concentrations of
Tacrolimus and Cyclosporine: A Retrospective Observational Study. Ther Drug Monit. 2016.12; 38(6);717-721[Conference Activities & Talks]
1. M. Nagata, M. Nakajima, Y. Ishiwata, Y. Takahashi, H. Takahashi, M. Yasuhara. Pharmacokineticpharmacocynamic modeling of olanzapine-induced acute hyperglycemia in rats. ISSX2016 2016.06.12
Busan, Korea
2. Ishiwata Y, Nagata M, Arai T, Makiishi M, Yoshikawa M, Takahashi H, Kohsaka H, Yasuhara M. Drugdrug Interaction of Miconazole Oral Gel with Tacrolimus and Cyclosporine After Oral Administration. ２
０１６ AAPS Annual Meeting and Exposition 2016.11.15 Denver, USA― 189 ―

Comprehensive Patient Care

Comprehensive Patient Care

Medical Education Research and Development

Professor Yujiro TANAKA
Junior Associate Professor Makoto TAKAHASHI
Junior Associate Professor Yasuhiro ITSUI
Junior Associate Professor Toshifumi KUDO
Junior Associate Professor Eriko OKADA
Senior Resident Fukiko KITAHATA(1)

OutlineDepartment of General Medicine was established in 2000, when Prof. Yujiro Tanaka assumed the role of chairman of the department. Since then, our aim has been to coordinate and support a wide range of new innovations
for the department of medicine and its aﬃliated hospitals. Accordingly, we launched the following projects tocarry out our mission; 1) Designing a new postgraduate clinical training program for TMDU aﬃliated hospitals,
2) Forming a patient support system including social casework, 3) Establishing the Center for Cell Therapy,
and 4) Reforming undergraduate medical education.
In response to the expansion of our activities, we have had some reforms in our organizations. 1) We founded
the Center for Postgraduate Medical Education in 2002. (Director: Prof. Tanaka. Associate Director: Dr.
Masanaga Yamawaki/former, Dr. Yoshihito Momohara/former, and Dr. Makoto Takahashi/previous) 2) In
2002, we also established the Center for Health and Welfare. And two years later in 2004, it was developed
into an independent center as the Department of Medicine when Dr. Masayoshi Shichiri was appointed as
the Director. 3) The Center for Cell Therapy, which was ﬁrst established as a part of the Blood Transfusion
Department in March, 2001, became an independent organization in 2003. Then Dr. Tomohiro Morio became
the director. 4) Prof. Tanaka became a member of the Board of Education and worked at the committee forcurriculum renovations in the Department of Medicine. Then he became the chair of the Education Committee
in 2004.
In addition to the curriculum reforms, the Department of General Medicine has been in charge of early clinicaltraining, PBL implementation, supervising patient-doctor communication education, OSCE (objective structured clinical examination) preparation, and BSL (bedside learning). We have also promoted educational
alliances with Harvard University since 2002 and with Imperial College, London since 2003.
As mentioned above, a couple of years after their launch, the Center for Health and Welfare and the Center for
Cell Therapy became independent from the Department of General Medicine. Meanwhile, new working groups
were formed within the department in 2004; the Working Group for Ward Management and the Safety Management Committee. The Department of General Medicine also devised an evaluation system for the residency
training program (EPOC), which was later adopted as a national online evaluation system for postgraduate
clinical training. We are working in close cooperation with Center for Interprofessional Education which we
took in part of its establishment to materialize the interprofessional education introduced due to a revision of
new curriculum in 2011.
(2)

ResearchResearch on continuing education in clinical EBM (Tanaka)
Although the theory of EBM (Evidence-Based Medicine) has become common knowledge, there are many
practical problems yet to be solved. Research on teaching and assessment techniques for under-and post-― 190 ―

Comprehensive Patient Care

Comprehensive Patient Care
graduate clinical training are ongoing.

Medical risk education using the HAZOP method-through analyzing basic surgical procedure (Takahashi)
Structured risk analysis methods, HAZOP, are applied for medical risk management. We have also developed
computer software for risk analysis with HAZOP. As a method of medical education for medical risk as well,
HAZOP is a comprehensive method that is eﬀective in reducing medical errors.
Review of clinical training in postgraduate clinical education (Takahashi)
The performance evaluation system using EPOC, which is used in 60% of educational hospitals in Japan, wasprimarily developed at Tokyo Medical and Dental University. We applied this system to a clerkship program
to compare its educational eﬀect with that of a residency program.
General research on medical education (Tanaka, Takahashi, Itsui, Kudou, Okada)
We are developing a comprehensive research project regarding postgraduate medical education, primary care inrural regions, development of clinical competence, and a new PBL system.

(3)

Education

Undergraduate Education
As a division, which is responsible for the education of students and residents, our primary goal is to foster
doctors who have both a ’patient-centered perspective as a specialist’ and ’up-to-date knowledge as a generalist’. To achieve our goal, we are designing and oﬀering a continuing medical educational (CME) program forclerkship students, emphasizing on educational systems spreading among multiple departments. Since we think
it is crucial to foster medical prospective with a patient-centered perspective, we introduced an early exposure
course (MIC: Medical Introductory Course) for the 1st and 2nd year medical students, as well as some medicine
oriented English courses, including a special course titled "Language and Philosophy of Western Medicine" regarding some of the needs of this globalized era of medicine. In addition, we are managing a training course
for simulated patients who can contribute to medical education cooperating with the International Center for
Medical Education at the University of Tokyo. To improve the quality of clinical training, we are currently
developing an evaluation system for tutors and trainers.
Postgraduate Education (Clinical Training)
Our department has oﬀered the postgraduate clinical training since 2004 according to the new national residency system in Japan. We have also played an important role in developing the online evaluation system forpostgraduate clinical training (EPOC), which is used in 60% of education hospitals in Japan. Results of thequestionnaire in Match, 2013 showed one of the highest satisfaction rate among all national universities.
Postgraduate Education (Master s degree courses)
We have been oﬀering master s degree courses in Medical Administration since this MMA program started in
2004, and were in charge of two courses this year, Human resources management and Leadership in the
medical care.

(4)

Clinical Services & Other Works

Second Opinion (Itsui)
Our hospital is open to the public who ask for second opinions about their recommended treatments so that
we can continue to contribute to the provision of safe and high-quality advanced medical technology. Over 300
consultation cases have been performed for patients coming from other hospitals nationwide. The purpose of thissection is to assist the patients to exercise their right of self-determination and to be informed of new treatments
and diagnostic tests. To provide a qualiﬁed second opinion, we have organized the network of specialists in
TMDU.
Patient Safety (Kudou)
Dr.Ooka is the General Risk Manager of our university hospital, and our department regularly organizes seminars
and training courses. In collaboration with other departments (e.g., Skills Laboratory Center, Infectious Control
Committee, etc.), we are working for greater safety and quality of healthcare.

― 191 ―

Comprehensive Patient Care

(5)

Comprehensive Patient Care

Publications[Original Articles]
1. Zainuer A, Inoue Y, Kudo T, Toyofuku T, Igari K, Kawano T.. Usefulness of the tranfer function index
for diagnosing peripheral arterial disease in patients with arterial calciﬁcation. J Med Dent Sci. 2016;
63(1); 29-35
2. Igari K, Kudo T, Toyofuku T, Inoue Y.. A novel approach to infrainguinal arteries with chronic total
occlusions using the high frequency vibrational device. Int Angiol. 2016; 35(2); 212-216
3. Igari K, Kudo T, Toyofuku T, Inoue Y.. The outcomes of endovascular aneurysm repair with the chimney
technique for juxtarenal aneurysms. Ann Throac Cardiovasc Surg. 2016; 22(3); 174-180
4. Yamamoto Y, Igari K, Toyofuku T, Kudo T, Inoue Y.. Late stent graft infection after the emergency
endovascular repair of a secondary iliac artery-enteric ﬁstula treated with graft removal and in situ aortic
reconstruction using femoral veins. Ann Thorac Cardiovasc Surg. 2016; [ Epub ahead of print]
5. Yamamoto Y, Kudo T, Igari K, Toyofuku T, Inoue Y.. Radial artery aneurysm in the anatomical snuﬀ
box: A case report and literature review. Int J Surg Case Rep. 2016; 27; 44-47
6. Igari K, Kudo T, Toyofuku T, Inoue Y.. Relationship of inﬂammatory biomarkers with severity of peripheral arterial disease. Int J Vasc Med. 2016; 6015701
7. Igari K, Kudo T, Toyofuku T, Inoue Y.. A case of successful coil embolization for a late-onset type Ia
endoleak after endovascular aneurysm repair with the chimney technique. Case Rep Vasc Med. 2016;
5307416
8. Igari K, Kudo T, Toyofuku T, Inoue Y.. Endothelial dysfunction of patients with peripheral arterial
disease measured by peripheral arterial tonometry. Int J Vasc Med. 2016; 3805380
9. Igari K, Kudo T, Toyofuku T, Inoue Y.. The relationship between endothelial dysfunction and endothelial
cell markers in peripheral arterial disease. PLoS ONE. 2016; 11(11); e0166840
10. Ryohei Hayashi, Kiichiro Tsuchiya, Keita Fukushima, Nobukatsu Horita, Shuji Hibiya, Keisuke Kitagaki,
Mariko Negi, Eisaku Itoh, Takumi Akashi, Yoshinobu Eishi, Eriko Okada, Akihiro Araki, Kazuo Ohtsuka,
Shinji Fukuda, Hiroshi Ohno, Ryuichi Okamoto, Tetsuya Nakamura, Shinji Tanaka, Kazuaki Chayama,
Mamoru Watanabe. Reduced Human α-defensin 6 in Noninﬂamed Jejunal Tissue of Patients with Crohn’s
Disease. Inﬂamm. Bowel Dis. 2016.05; 22(5); 1119-1128[Misc]
1. Makoto Takahashi. Residency and board certiﬁcation of orthopaedic surgery in USA Orthopaedic Surgery
and Traumatology. 2016.09; 59(10); 1319-1332[Conference Activities & Talks]
1. Igari K, Kudo T, Toyofuku T, Inoue Y.. Relationship of inﬂammatory biomarkers, PTX3 with severityof peripheral arterial disease.. 84th European Atherosclerosis Society Congress 2016.05.31 Innsbruck(Austria)
2. Igari K, Kudo T, Toyofuku T, Inoue Y.. Total endovascular treatment for acute deep venous thrombosis
by catheter-directed thrombolysis.. Asian Society for Vascular Surgery 2016 2016.10.21 Singapore
3. Yamamoto Y, Ichinose T, Nakamura M, Nishizawa M, Igari K, Toyofuku T, Kudo T, Inoue Y.. Multiple
recurrent pseudoaneurysms after EVAR in a patient with Behçet s disease.. The 13th Tokyo Shanghai
International Symposium for Vascular Surgery 2016.11.13 Tokyo (Japan)― 192 ―

Comprehensive
Comprehensive Patient
Patient Care
Care

Acute Critical Care and Disaster Medicine

Professor Yasuhiro Otomo(1)

Outline1. Purpose of Education
We, the department of acute critical care and disaster medicine, investigate following wide range of ﬁelds, suchas the search for mechanisms of biological response to severe stresses, the development of strategy for multiple
organ dysfunction from the view of intensive care medicine, basic and clinical research about trauma, trauma
preventive medicine and disaster medicine. Our targets of research are practical and cutting edge to work notonly as a medical scientist but as a researcher for government projects.
2. Research Subjects
Basic research of the mechanism of multiple organ dysfunction following hemorrhagic/septic shock
Development of strategy for multiple organ dysfunction
Basic and clinical research of multiple trauma
Trauma epidemiology and trauma preventive medicine
Disaster medicine
Clinical research of cerebrovascular disease on acute phase
3. Clinical Services
Our emergency center was authorized to hold the 21st level I center in Tokyo on April 1, 2007. We givetreatments over 8000 patients annual who are under critical condition like multiple organ dysfunction, severe
sepsis and septic shock, life-threatening trauma as well. We also contribute to medical services, rushing to theemergency scene by a Doctor-Car/Helicopter at times.

(2)

Publications[Original Articles]
1. Junichi Inoue, Atsushi Shiraishi, Ayako Yoshiyuki, Koichi Haruta, Hiroki Matsui, Yasuhiro Otomo. Resuscitative endovascular balloon occlusion of the aorta might be dangerous in patients with severe torso
trauma: a propensity score analysis. J Trauma Acute Care Surg. 2016.01;
2. Mukawa, M. Nariai, T. Inaji, M. Tamada, N. Maehara, T. Matsushima, Y. Ohno, K. Negi, M. Kobayashi,
D.. First autopsy analysis of a neovascularized arterial network induced by indirect bypass surgery formoyamoya disease J Neurosurg. 2016.05; 124(5); 1211-1214
3. Akira Endo, Atsushi Shiraishi, Shigeki Kushimoto, Yasushiro Otomo. Veriﬁcation of conventional criteriaof the lethal triad and development of novel criteria as an indicator of decision making
4. Akira Endo, Atsushi Shiraishi, Yasuhiro Otomo, Shigeki Kushimoto. Development of novel criteria ofthe lethal triad as an indicator of decision making in current trauma care: a retrospective multicenter
observational study in Japan Critical Care Medicine.

― 193 ―

Comprehensive Patient
Patient Care
Care
Comprehensive[Conference Activities & Talks]
1. Jun-ichi Inoue, Atushi Shiraishi, Ayako Yoshiyuki, Koichi Haruta, Yasuhiro Otomo. RESUSCITATIVEENDOVASCULAR BALLOON OCCLUSION OF THE AORTA (REBOA) MIGHT BE DANGEROUS
IN PATIENTS WITH SEVERE TORSO TRAUMA ─ A PROPENSITY SCORE ANALYSIS SAYS ─.
74th Annual Meeting of AAST and Clinical Congress of Acute Care Surgery 2016.09.09 Las Vegas, NV,
United States― 194 ―

Comprehensive
Comprehensive Patient
Patient Care
Care

Clinical Oncology

Professor MIYAKE Satoshi
Project Associate Professor
OOOKA Shinya
Project Assistant Professor
SAKASHITA Hiroyuki
Project Assistant Professor
ITOU Hiromitsu
Graduate Student
SATOU Noriyuki(1)

OutlineDepartment of Clinical Oncology was established in May 2012 to promote the ﬁeld of palliative medicine andcancer chemotherapy according to Training Program for Next Generation Specialists to Promote Cancer
Therapy . As for the education in medical school, we are involved in the course of Hematology-Oncology
block and have a class of palliative medicine in the third year grade. In addition, we have a class of clinicalethics mainly focusing on the end-of-life care. As for the post-graduate education, we organized the Training
Program for Next Generation Specialists to Promote Cancer Therapy .
(2)

Research

1)Application of palliative care when the patient is diagnosed as cancer.
2)Improvement of QOL in the end-of -life care of cancer patients.
3)Communication skills in the team health care.
4)Multi-institutional research in pancreatic cancer treatment.
5)The role of biomarkers for newly developed anti-cancer drugs in lung cancer.
(3)

Education1)Hematology/Oncology2)GI tract cancer3)Lung cancer4)Ethics5)Communication(4)

Clinical Services & Other Works

Department of Clinical Oncology manages Cancer Center of the medical school hospital.
There are ﬁve divisions below.
1)Division of palliative medicine― 195 ―

Comprehensive Patient
Patient Care
Care
Comprehensive2)Division of cancer chemotherapy3)Division of cancer registory4)Division of coordination of cancer treatment
5)Division of cancer consultation and support(5)

Publications[Original Articles]
1. Nakajima Y, Kawada K, Tokairin Y, Tomita M, Miyake S, Kawano T.. Prognostic Factors for PostRecurrence Survival in Patients with Thoracic Esophageal Squamous Cell Carcinoma after Curative Resection. Dig Surg. 2016; 33(2); 136-145
2. Retrospective Analyses of Esophageal Bypass Surgery for Patients with Esophagorespiratory Fistulas
Caused by Esophageal Carcinomas. World J Surg. 2016.05; 40(5); 1158-1164― 196 ―

Comprehensive
Comprehensive Patient
Patient Care
Care

Dentistry for Persons with Disabilities

Associate Professor
Osamu SHINOZUKA
Junior Associate Professor（Part-time）
Minoru INADA
Goro SEKIGUCHI
Naoki HAYASHI
Yohei TAKEUCHI
Syohei TAMURA
Moriyuki NAKAMURA
Assistant Professor
Yasuka KUSUMOTO
Graduate Student
Aiko HOSHIAI
Hospital Staﬀ
Taiji HOSHIAI
Ayana NATORI
Anna KUMAKUrA
Hirotoshi Yamawaki
Graduate International Research Student
Shohei TAKAHASHI
Clinical Junior Associate Professor（Part-time）
Seiji SAKURAI
Tomo SUZUKI
Yosuke KINOSHITA(1)

Research

1) Formation of oral bioﬁlm2) Elimination of oral bioﬁlm of persons with disabilities
3) Oral health status of the medically compromised patients
4) Oral management of genetic syndrome(2)

Lectures & Courses

Our department was started as a graduate course of the special dentistry section on April, 1999. The sections
are the dentistry for persons with disabilities and medically compromised individuals .
The main objective of this course is to provide the opportunity for students to understand the outline of thereconstruction of functional and esthetic disorders of oral and/or maxillofacial areas by means of the high-― 197 ―

Comprehensive Patient
Patient Care
Care
Comprehensive

advanced dental cares for patients with special needs.

(3)

Clinical Performances

The clinical purpose of our department is to treat oral problems of special patients who are unable toreceive normal dental care by reason of a disability which may be physical, mental, medical, or emotional, or
combination of any of these under using behavior management and systemic support.
For example,
1) The patients requiring behavior management are physically disabled, intellectual disability, autistic spectrum
disorder, etc.
2) The patients requiring systemic support are internal impediment, dental phobia, etc.

(4)

Publications[Original Articles]
1. Takutoshi Inoue, Tomoyuki Miyamoto, Haruhisa Fukayama, Yasuka Kusumoto, Osamu Shinozuka. Intravenous Sdation for Dental Treatment in a Patient with Wiskott-Aldrich Syndrome Jourmal of Japanese
Dental Society of Anesthesiology. 2016.01; 44(1); 17-19― 198 ―

Comprehensive
Comprehensive Patient
Patient Care
Care

General Dentistry

Associate Professor Shigeru ODA
Junior Associate Professor Masayuki HIDESHIMA
Junior Associate Professor Satoko OHARA
Junior Associate Professor Ken-ichi TONAMI
Assistant Professor Azusa YAMADA
Assistant Professor Sachi UMEMORIAssistant Professor Kanako NORITAKE
Hospital Staﬀ Shuuhei NAKAMURAI
Hospital Staﬀ Yuko MITSUMA
Hospital Staﬀ Mina GOTO
Hospital Staﬀ Maiko IWAKI
Hospital Staﬀ Yasuyuki KIMURA
Hospital Staﬀ Shota HAYASHI
Hospital Staﬀ Naoki ISHIHARA
Hospital Staﬀ Shogo MAEKAWA(1)

OutlineRecent dentistry is sectionalized into various specialized ﬁelds of research and education. On the other hand, asa general practitioner, a dentist must possess not only integrated knowledge and skills of all the ﬁelds but also
should be competent to apply such generalized knowledge and skills to individual patients. The department ofGeneral Dentistry performs researches and education for practicing such general and holistic dentistry. General
dentistry related to clinical and aﬀective education for dental students and residents. Therefore, the research
theme includes dental education as well as oral diagnosis and general dentistry, cooperating with the departmentof Educational System in Dentistry, Behavioral Dentistry and Educational Media Development. Researches forsleep apnea syndrome have been also conducted in association with Dental Clinic for Sleep Disorders. The
clinic of department of General Dentistry is Oral Diagnosis and General Dentistry which missions in the Dental
Hospital are initial diagnosis for new outpatients and general dental practice. Clinics of General Dentistry 1
and 2, where dental students and residents are trained, also relate to the department of General Dentistry.

(2)

Research

Recent research themes are listed below.
1. Study for structure of health problems and treatment planning for dental patients.
2. Study for veriﬁcation and improvement of oral diagnosis.
3. Study for dental education for dental students and residents.

― 199 ―

Comprehensive Patient
Patient Care
Care
Comprehensive(3)

Education

The educational objective of General Dentistry is that the students/residents acquire transversal academic
framework of dental knowledge and skills and competency to provide patients with personalized treatment.
(4)

Lectures & Courses

1. Introduction to the Behavioral Science. (For the 2nd-year dental students)
2. Holistic Oral Diagnosis. (For the 5th-year dental students)
3. Comprehensive Clinical Training Phase Ⅰ Phase Ⅱ. (For the 5th and 6th-year dental students)
4. Clinical training (For the dental residents)(5)

Clinical Services & Other Works

The clinic of department of General Dentistry is Oral Diagnosis and General Dentistry. In the clinic, dental
examination and health assessment for new outpatients are conducted to decide initial treatment plan and aclinic in charge for the patients. The patients who need comprehensive dental care and who cannot cooperate
students’ practice also attend this clinic to receive general dental practice.

(6)

Clinical Performances

In the Dental Hospital, the clinic of Oral Diagnosis and General Dentistry is the ﬁrst clinic for new outpatients.
That is, the clinic is responsible for constructing good relationship between patients and the hospital. Therefore,
the doctors pay attention actively to psychosocial aspects of patients during examination. In diagnosing anddeciding clinic for patients, initial-treatment plans are introduced to patients. In this process, doctors thinktreatment plans together with patients thoroughly to obtain sound informed consent. Many patients who visits
the dental hospital demands high medical level of the university hospital while not a few patients needs primarycare. Oral Diagnosis and General Dentistry provides such patients with general dental practice to meet various
kinds of patients’ needs, makes eﬀort to improve patients’ satisfaction.
(7)

Publications[Original Articles]
1. Yuka Tsumanuma, Takanori Iwata, Atsuhiro Kinoshita, Kaoru Washio, Toshiyuki Yoshida, Azusa Yamada, Ryo Takagi, Masayuki Yamato, Teruo Okano, Yuichi Izumi. Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size SupraAlveolar Periodontal Defect Model BioResearch Open Access. 2016.01; 5(1); 22-36
2. Hoshi S, Akizuki T, Matsuura T, Ikawa T, Kinoshita A, Oda S, Tabata Y, Matsui M, Izumi Y . Ridge
augmentation using recombinant human ﬁbroblast growth factor-2 with biodegradable gelatin sponges incorporating β-tricalcium phosphate: a preclinical study in dogs Journal of Periodontal Research. 2016.01;
51(1); 77-85
3. Ikawa T, Akizuki T, Matsuura T, Hoshi S, Ammar S, A, Kinoshita A, Oda S, Izumi Y. Ridge Preservation
After Tooth Extraction With Buccal Bone Plate Deﬁciency Using Tunnel Structured Β-Tricalcium Phosphate Blocks: A 2-Month Histological Pilot Study in Beagle Dogs Journal of Periodontology. 2016.02;
87(2); 175-183
4. Kurosa Michiyo, Tonami Ken-ichi, Ohara Satoko, Umemori Sachi, Noritake Kanako, Sunaga Masayo,
Kinoshita Atuhiro and Araki Kouji. Diﬀerennces between Computer-Based and Paper-Based Assessmentsof the Clinical Reasoning Competency of Dental Students The Journal of The Stomatological Society,Japan. 2016.03; 83(1); 25-33
5. Komagamine Y, Kanazawa M, Iwaki M, Jo A, Suzuki H, Amagai N, Minakuchi S. Combined eﬀect of newcomplete dentures and simple dietary advice on nutritional status in edentulous patients: study protocol
for a randomized controlled trial. Trials. 2016.11; 17(1); 539-546― 200 ―

Comprehensive
Comprehensive Patient
Patient Care
Care

[Books etc]
1. Shunsuke Minakuchi, Manabu Kanazawa, Norihisa Akiba, Yusuke Sato, Maiko Iwaki, Mai Okubo, Yuriko
Komagamine. The live lecture of the complete denture -The ﬁrst step of the full mouth reconstruction-.
2016.05
2. Izumi Y, Nikaidou M. Correspondence to vertical bone defects. Igaku Joho-sha, 2016.10[Misc]
1. Masayuki Hideshima, Syuhei Nakamura, Hisashi Matsubara, Tatsu Suzuki. Oral Appliances for Sleep
Apnea Syndrome The Journal of the Japanese Society for Dental Materials and Devices, The Journal of
Dental Engineering. 2016.01; 35(1); 10-13
2. Manabu Kanazawa, Maiko Iwaki, Shinta Yamamoto, Toshio Arakida and Shunsuke Minakuchi. The
complete denture with using CAD/CAM system 2016.06; 35(3); 185-188
3. ODA Shigeru and HOTTA Yasunori. Preface: With a View to Reconsidering Peri-Implant Disease:
Prevention and Treatment Journal of Japanese Society of Oral Implantology. 2016.12; 29(4); 207[Conference Activities & Talks]
1. Jo A,Kanazawa M,Sato Y,Akiba N,Iwaki M, Komagamine Y, Minakuchi S. Inﬂuence of the diﬀerent
impression methods for complete denture fabrication on masticatory ability. 2016.01.24
2. Arakida T,Kanazawa M,Yamamoto S,Iwaki M, Minakuchi S,Suzuki T. The eﬀect if external light of digital
impression. The eﬀect of external light of digital impression 2016.05.29
3. M. Kanazawa, M. Iwaki, D. Sato, Y. Omura, A. Miyayasu, S. Kasugai, S. Minakuchi.. Immediate Loadingof two-implants mandibular overdentures: 4-years prospective study.. 94th General Session & Exhibitionof the IADR 2016.06 Seoul, Republic of Korea
4. Tonami K,Sano K,Iwasaki N,Takahashi H,Araki K. TEM image of dentin after Xe excimer lamp irradiation. The 144th Meeting of the Japanese Society of Cnsevative Dentistry 2016.06.09
5. Anna Miyayasu, Ayami Jo, Manabu Kanazawa, Yusuke Sato, Maiko Iwaki, Norihisa Akiba, Shunsuke
Minakuchi. Cost Analysis of the diﬀerent impression methods for complete dentures. 94th General
Session & Exhibition of the IADR 2016.06.25 Seoul, Korea
6. Ken-ichi TONAMI, Azusa YAMADA, Sachi UMEMORI, Kanako NORITAKE, Yasuyuki KIMURA1,
Maiko IWAKI, Masayo SUNAGA, Masayuki HIDESHIMA, Shigeru ODA, Hiroshi NITTA, Atsuhiro KINOSHITA, Kouji ARAKI, Shiro MATAKI. Inﬂuential factor in medical ethics practice on class evaluation
by students after three years. 2016.07.01
7. Hiroyuki Ishiyama, Shusuke Inukai, Akira Nishiyama, Masayuki Hideshima, Shuhei Nakamura, Chisato
Ida, Yuko Mitsuma, Shota Hayashi, Akihito Uesato, Toshihide Fujie, Meiyo Tamaoka, Yasunari Miyazaki,
Masahiko Shimada, Noriyuki Wakabayashi . Eﬀect of jaw-opening exercise on prevention of temporomandibular disorders pain associated with oral appliance therapy and compliance in obstructive sleep
apnea patients: A randomized, double-blind, placebo-controlled trial.. Japanese Society of Sleep Researchthe 41th Annual Meeting 2016.07.07 Tokyo
8. Multicenter Randomized Clinical Study of Denture Adhesive to Establish the Guideline: Study by the
Subgroup Analysis for a Denture Adhesive on the Eﬀect of the Masticatory Performance. 2016.07.09
9. Arakida T,Kanazawa M,Yamamoto S,Iwaki M, Minakuchi S,Suzuki T,Oki M,Kamijo S,Ando K,Morisawa
K. The eﬀect of external light of digital impression time and accuracy. 2016.07.09
10. Miyayasu A, Jo A, Kanazawa M, Sato Y, Iwaki M, Akiba N, Minakuchi S. Cost-eﬀectiveness analysisof the impression methods for mandibular complete dentures in randomized controlled trial. The 125th
Annual Meeting of the Japan Prosthodontic Society 2016.07.09 Kanazawa
11. Hiroyuki Suzuki,Manabu Kanazawa,Yuriko Komagamine,Maiko Iwaki,Ayami Jo,Noriko Amagai,Shunsuke
Minakuchi. Combined eﬀect of new complete dentures and simple dietary counseling on nutritional statusin edentulous patients : study protocol for a randomized controlled trial. The 125th Scientiﬁc Meeting ofJapan Prosthodontic Society 2016.07.09 Kanazawa― 201 ―

Comprehensive Patient
Patient Care
Care
Comprehensive

12. Wada J,Hideshima M, Inukai S, Matsuura H, Wakabayashi N. Inﬂuence of shape of dental arch and palate
on the accuracy of speech production disturbed by major connector of maxillary removable partial denture.
The 125th Annual Meeting of the Japan Prosthodontic Society 2016.07.09 Kanazawa
13. Akira Aoki, Koji Mizutani, Yoichi Taniguchi, Motohiro Komaki, Shigeru Oda, Hisashi Watanabe, Yuichi
Izumi. Er:YAG Laser-assisted Comprehensive Periodontal Pocket Therapy (Er-LCPT): Case Series. The
15th Congress of the World Federation for Laser Dentistry 2016.07.17 Nagoya、Japan
14. Jun Tsuruta, Kanako Noritake, Kouji Mizutani, Naoko Seki, Keiko Kondou, Shin-ichi Arakawa, Kouji
ARAKI. A Trial for a new IPW course for Dental students and Oral health sciences students working
in a dental team . Association of Dental Education in Europe, Annual Conference 2016.08.25 Balcelona
15. Jun Tsuruta, Kanako Noritake, Kouji Mizutani, Naoko Seki, Keiko Kondou, Shin-ichi Arakawa, Kouji
ARAKI. A new IPW course working in a dental team. 27th Annual meeting, Southeast Asia Association
for Dental Education 2016.09.08 Ho chi ming city
16. Son Hoang le, Ly Thi-Bich Nguyen, Lan Thi-Quynh Ngo, Sachi Umemori, Ken-ichi Tonami, Shiro Mataki.
Impact of preparatory information on patient undergoing third molar surgery. 27th SEAADE Annual
Scientiﬁc Meeting 2016.09.08 Ho Chi Minh City, Viet Nam
17. Maekawa S, Katagiri S, Takeuchi Y, Komazaki R, Ohtsu A, Udagawa S, Izumi Y. Bone metabolic microarray analysis of ligature-induced periodontitis in streptozotocin- induced diabetic mice. 102nd Annual
Meeting of American Academy of Periodontology 2016.09.10 San Diego, USA
18. Kenichi Tonami, Yasuyuki Kimura, Miyuki Umemori, Masayuki Hideshima, Shiro Mataki, Kouji Araki.
Basic reseach for morphological diagnosis using fractal dimension. 2016.09.22
19. Jun Tsuruta, Kanako Noritake, Koji Mizutani, Naoko Seki, Keiko Kondo, Shinichi Arakawa, Kouji Araki.
Implementing a new IPW course in the dental student clinic . The 5th Tri-University Consortium on Oral
Science and Education 2016.10.27 Beijing, China[Awards & Honors]
1. Naoki Ishihara. JADSM KIKUCHI Award 2016, 2016.11― 202 ―

Comprehensive
Comprehensive Patient
Patient Care
Care

Psychosomatic Dentistry(1)

Professor

Akira Toyofuku

Assistant Professor

Miho Takenoshita

Hospital Staﬀ

Takeru Kyuragi, Anna Suzuki-Miura, Yojiro Umezaki

Graduate Student

Yukiko Shinohara, Anna Suzuki-Miura, Rou Mikuzuki
Kaoru Kawasaki,Shiori Sugawara, TU Thi Huyen Trang

Lecturer（part-time）

Haruhiko Motomura, Ayano Katagiri, Tatsuya Yoshikawa

OutlinePsychosomatic dentistry is the only one department in Japan, which research and develop new diagnosis andtreatment methods for MUOS such as BMS, AO, PBS etc.
(2)

Research

1)Study on pathophysiological mechanisms of oral psychosomatic disorders2)Psychosomatic study on oro-facial medically and psychiatrically unexplained symptoms
3) Brain imaging of oral psychosomatic disorders4)Psychopharmacological study on oral psychosomatic disorders(3)

Education

It is not uncommon to see the patients diagnosed with Oral Psychosomatic Disorders , so there is a growing
need for proper treatment of the disorders from both sides of doctors and patients. It is, therefore, extremely
important for dental students to instruct in psychosomatic dentistry. However, few Dental Universities in Japanare following this. At the same time, there s a great deal of misunderstanding about psychosomatic dentistry,in spite of we have many years of consistent education. For example, Your work is only hearing to complaints
from patients , Patients with not otherwise speciﬁed mental illness is eventually referred to your clinic , or
The mission of your clinic is to calm down your patients with unidentiﬁed dental and oral complaints .
So, regarding undergraduate medical education, we focus on not only lessons from lectures and books but also
practical experience trough clinical training. We have comprehensive medical teaching for ﬁfth and sixth-year
students. Students can listen to patient s complaints directly and deepen their understanding. Actually
they can see patients with dental psychosomatic disorders, and they know that these disorders are treatable.
Moreover, they can learn negative eﬀects of wrong ideas as a psychogenic disorder, and they can understandserious distress in patients and family members.
This practice is arduous eﬀort, but in the future, it is hoped that eﬀorts will be made to facilitate uniformed
services for patients with dental psychosomatic disorders, enhance coping skills for refractory cases, and reducetrouble with patients by the graduates of our department who mastered psychosomatic dentistry.
It is important to have identity as a dentist on practice of psychosomatic dentistry. Therefore we have advanced― 203 ―

Comprehensive Patient
Patient Care
Care
Comprehensive

strengthening of human resource development. In particular, we focus on cultivation of dentists who can bereadily applied their knowledge of psychosomatic medicine to clinical practice. And we are working towards
establishment of psychosomatic dentistry introduced psychotherapy.
Also regarding education for graduate student, we focus on clinical practice for development of dentists who
have great skill in psychosomatic dentistry.

(4)

Clinical Services & Other Works

We take charge of Psychosomatic Dentistry clinic in dental hospital of Tokyo Medical and Dental University. This special clinic is for patients with oral psychosomatic disorders, such as glossodynia (burning mouth
syndrome), atypical facial pain, atypical odontalgia, oral dysesthesia, occlusal discomfort(dysesthesia).
Main psychosomatic treatment is psychopharmacological one with SSRIs(Selective Serotonin Reuptake Inhibitors), SNRI(Serotonin-Noradrenaline Reuptake Inhibitor), SDAs(Serotonin-Dopamin antagonists) etc. And
supportive psychotherapies are applied.
Intractable cases are increasing year by year, we take care of every patient and have good clinical courses about
70% of them.
We believe there are exactly oral psychosomatic disorders , and dentists should be in charge of treatment.
Psychosis, as a matter of course, should be taken care by psychiatrists, so we discriminate them from oral
psychosomatic disorders, and properly refer to psychiatry.
On the other hand, on functional somatic symptoms secondary to psychiatry disorders , which are refer tous from psychiatrists, we do our best in cooperation with psychiatrists.
We have about 600 new outpatients per year, and almost of them were referred from other specialists not
only in dentistry but also internal medicine, otorhinolaryngology, dermatology, psychosomatic medicine, andpsychiatry. They come from the Metropolitan area, of course, Osaka, Kyushu, Hokkaido and so on. We takeﬁne-grained care and follow up, total number of patients is up to 10,000 per year.
We have a mission to meet the demand of these patients and their families, so better treatment outcome andincreasing eﬃciency are required, and cooperation with other medical specialists is needed.

(5)

Clinical Performances

Psychosomatic dentistry clinic is very unique, specialized for patients with MUOS. We have treated a large
number of patients with various oral psychosomatic problems. With our unrivalled clinical experiences for
MUOS, we oﬀer our best clinical setting for the treatments of every MUOS.
(6)

Publications[Original Articles]
1. Yojiro Umezaki, Bashar W Badran, William H DeVries, Jkeonye Moss, Theresa Gonzales, Mark S George.
The Eﬃcacy of Daily Prefrontal Repetitive Transcranial Magnetic Stimulation (rTMS) for Burning Mouth
Syndrome (BMS): A Randomized Controlled Single-blind Study. Brain Stimul. 2016.03; 9(2); 234-242
2. Yojiro Umezaki, Miho Takenoshita, Akira Toyofuku. Low-dose aripiprazole for refractory burning mouth
syndrome. Neuropsychiatr Dis Treat. 2016.05; 12; 1229-1231
3. Miho Takenoshita, Haruhiko Motomura, Akira Toyofuku. Low-Dose Aripiprazole Augmentation in AmitriptylineResistant Burning Mouth Syndrome: Results from Two Cases. Pain Med. 2016.08;[Misc]
1. Yojiro Umezaki, Anna Miura, Motoko Watanabe, Miho Takenoshita, Akihito Uezato, Akira Toriihara,
Toru Nishikawa, Akira Toyofuku. Oral cenesthopathy. Biopsychosoc Med. 2016; 10; 20
2. Akira Toyofuku. Psychosomatic problems in dentistry. Biopsychosoc Med. 2016.04; 10; 14― 204 ―

Comprehensive
Comprehensive Patient
Patient Care
Care

[Conference Activities & Talks]
1. Akira Toriihara, Shin Nakamura, Akira Toyofuku, Ukihide Tateishi. Usefulness of Molecular Imaging for
Patients with Dental Diseases.. SNMMI annual meeting 2016.06.13 San Diego, California, USA
2. Yojiro Umezaki, Anna Miura, Yukiko Shinohara, Lou Mikuzuki, Shiori Sugawara, Kaoru Kawasaki, Takeru
Kyuragi, Motoko Watanabe, Miho Takenoshita, Akira Toyofuku . Clinicostatistical Study on the Patients with Oral Cenesthopathy. 17th Congress of the Asian College of Psychosomatic Medicine (ACPM)
2016.08.21 Fukuoka, Japan
3. Miho TAKENOSHITA, Anna MIURA, Yukiko SHINOHARA, Lou MIKUZUKI, Kaoru KAWASAKI, Shiori SUGAWARA, Trang THI HUYEN YU, Takeru KYURAGI, Yojiro UMEZAKI,Motoko WATANABE,
Ayano KATAGIRI, Tatsuya YOSHIKAWA, Akira TOYOFUKU. Two Cases of Atypical Odontalgia Successfully Treated by Adding Low-Dose of Aripiprazole to Mirtazapine. 17th Congress of the Asian Collegeof Psychosomatic Medicine (ACPM) 2016.08.21 Fukuoka, Japan
4. Motoko Watanabe, Anna Miura, Yukiko Shinohara, Lou Mikuzuki, Yojiro Umezaki, Takeru Kyuragi,
Tatsuya Yoshikawa, Miho Takenoshita, Akira Toyofuku . Psychopharmacological Outcomes and Its Predictors for Burning Mouth Syndrome: 2 Years Follow Up Study. 17th Congress of the Asian College of
Psychosomatic Medicine (ACPM) 2016.08.21 Fukuoka, Japan
5. Anna Miura, Yukiko Shinohara, Lou Mikuzuki, Shiori Sugawara, Kaoru Kawasaki,Tu Thi Huyen Trang,
Yojiro Umezaki, Miho Takenoshita, Akira Toyofuku . Psychiatric Comorbidities in Patients with Persistent
Dento-Alveolar Pain Disorder (Atypical Odontalgia). 17th Congress of the Asian College of Psychosomatic
Medicine (ACPM) 2016.08.21 Fukuoka, Japan― 205 ―

Comprehensive Patient Care

Comprehensive Patient Care

Behavioral Dentistry

Professor
Shiro Mataki
Associate Professor
Hiroshi Nitta
Research Associate
Azusa Yamada
Research Associate
Sachi Umemori
Graduate Student
Ayako Kubota
Graduate Student
Le Son Hoang (Vietnam)
Graduate Student
Shizuka Tanaka(1)Research

1）Construction of educational system of behavioral dentistry for dental students2）Application of behavioral science to development of dental educational curriculum3）Patients’ evaluation of the dental hospital and the dental educational system4）Application of behavioral science to dental clinic(2)

Lectures & Courses

Topic of Behavioral Dentistry included characteristics of human behavior, especially of relationship between
patients and dental staﬀ based on the informed consent. Main objective of behavioral dentistry in the graduate
course is to provide students opportunity to study application of behavioral science to deal with dental patients
showing various perception and behavior in clinic.
(3)

Clinical Services & Other Works

Behavioral Dentistry provides medical interview for preliminary diagnosis and general dental practice at theclinic of oral diagnosis and general dentist cooperating with General Dentistry.
(4)

Publications[Original Articles]
1. Yuka Tsumanuma, Takanori Iwata, Atsuhiro Kinoshita, Kaoru Washio, Toshiyuki Yoshida, Azusa Yamada, Ryo Takagi, Masayuki Yamato, Teruo Okano, Yuichi Izumi. Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size SupraAlveolar Periodontal Defect Model BioResearch Open Access. 2016.01; 5(1); 22-36
2. Michiyo Kurosa, Ken-ichi Tonami, Satoko Ohara, Sachi Umemori, Kanako Noritake, Masayo Sunaga,
Atsuhiro Kinoshita, Kouji Araki. Diﬀerences between Computer-Based and Paper-Based Assessments of
the Clinical Reasoning Competency of Dental Students. Kokubyo Gakkai Zasshi. 2016.03; 83(1); 25-33
3. Seki N, Moross J, Sunaga M, Hobo K, Miyoshi T, Nitta H, Kinoshita A, Morio I. Evaluation of simulation
learning materials use to ﬁll the gap in Japanese dental English education. Journal of Medical and Dental
Sciences. 2016.03; 63(1); 1-8― 206 ―

Comprehensive Patient Care

Comprehensive Patient Care

4. Yuki Ohara, Hirohiko Hirano, Hideyo Yoshida, Shuichi Obuchi, Kazushige Ihara, Yoshinori Fujiwara,
Shiro Mataki. . Prevalence and factors associated with xerostomia and hyposalivation among communitydwelling older people in Japan. Gerodontology. 2016.03; 33(1); 20-27
5. Yuki Ohara, Naomi Yoshida, Hisashi Kawai, Shuichi Obuchi, Hideyo Yoshida, Shiro Mataki, Hirohiko
Hirano, Yutaka Watanabe. Development of an oral health-related self-eﬃcacy scale for use with older
adults. Geriatr Gerontol Int. 2016.08;[Books etc]
1. Hiroshi Nitta, Sayaka Katagiri. Happening recovery in periodontal surgery. Quintessence PublishingCo.,Ltd, 2016.11 (ISBN : 978-4-7812-0522-9)[Conference Activities & Talks]
1. Ayako Kubota, Yoko Kono, Hiroko Imura, Hiroji Shimomura, Kiyoko Kanamori, Yasuko Kawakami, Shiro
Mataki, Kumiko Sugimoto. Oral health status and behavior of Japanese university students. International
Symposium on Dental Hygiene 2016 2016.06 Basel, Switzerland
2. Naomi Yoshida, Kumiko Sugimoto, Sato Yamanaka, Hiroyuki Sakamaki, Yoko Yamazaki, Asako Kimura,
Hiroko Imura, Yoko Kono, Ayako Kubota. Oral and holistic health evaluation and dental health status
in dental hygienists at menopausal stage. International Symposium on Dental Hygiene 2016 2016.06.23
Basel
3. Ken-ichi TONAMI, Azusa YAMADA, Sachi UMEMORI, Kanako NORITAKE, Yasuyuki KIMURA1,
Maiko IWAKI, Masayo SUNAGA, Masayuki HIDESHIMA, Shigeru ODA, Hiroshi NITTA, Atsuhiro KINOSHITA, Kouji ARAKI, Shiro MATAKI. Inﬂuential factor in medical ethics practice on class evaluation
by students after three years. 2016.07.01
4. Son Hoang le, Ly Thi-Bich Nguyen, Lan Thi-Quynh Ngo, Sachi Umemori, Ken-ichi Tonami, Shiro Mataki.
Impact of preparatory information on patient undergoing third molar surgery. 27th SEAADE Annual
Scientiﬁc Meeting 2016.09.08 Ho Chi Minh City, Viet Nam
5. Kenichi Tonami, Yasuyuki Kimura, Miyuki Umemori, Masayuki Hideshima, Shiro Mataki, Kouji Araki.
Basic reseach for morphological diagnosis using fractal dimension. 2016.09.22― 207 ―

Comprehensive Patient Care

Comprehensive Patient Care

Professional Development in Health Sciences

Professor Kazuki Takada
Associate Professor Jun Tsuruta
Associate Professor Mina Nakagawa
Junior Associate Professor Kumiko Yamaguchi(1)

OutlineWorldwide, accelerated aging and the shift in disease burdens have created a demand for innovations in healthsciences, healthcare, and the healthcare delivery system. Innovation requires not only a vast amount of knowledge and superior skills, but also critical and creative thinking skills. Innovation concerning new drugs and medical devices further requires understanding of the entire ﬂow and process of research and development. In our department, we provides educational opportunities for learners to acquire high-level and practical knowledge of thefollowings: history of medical and dental education in Japan, professional education/development/certiﬁcationin Japan and North American/European countries, key pedagogical theories and learning methods, processbased approach and logic models in curriculum development, and competencies and their assessment/evaluation.
(2)

Research· Needs assessment in health care and in professional development in health science ﬁelds · Curriculum
development for professionals of the future needs in health sciences· Interprofessional education curriculum development(3)

Clinical Services & Other Works

Medical Hospital
Kazuki TAKADA : Rheumatology
Mina NAKAGAWA : Gastroenterology and Hepatology
Dental Hospital
Jun TSURUTA : Oral Diagnosis and General Dentistry(4)

Publications[Original Articles]
1. Satoshi Otani, Sei Kakinuma, Akihide Kamiya, Fumio Goto, Shun Kaneko, Masato Miyoshi, Tomoyuki
Tsunoda, Yu Asano, Fukiko Kawai-Kitahata, Sayuri Nitta, Toru Nakata, Ryuichi Okamoto, Yasuhiro Itsui,
Mina Nakagawa, Seishin Azuma, Yasuhiro Asahina, Tomoyuki Yamaguchi, Naohiko Koshikawa, Motoharu
Seiki, Hiromitsu Nakauchi, Mamoru Watanabe. Matrix metalloproteinase-14 mediates formation of bileducts and hepatic maturation of fetal hepatic progenitor cells. Biochem. Biophys. Res. Commun.
2016.01; 469(4); 1062-1068
2. Fukiko Kawai-Kitahata, Yasuhiro Asahina, Shinji Tanaka, Sei Kakinuma, Miyako Murakawa, Sayuri Nitta,
Takako Watanabe, Satoshi Otani, Miki Taniguchi, Fumio Goto, Hiroko Nagata, Shun Kaneko, Megumi― 208 ―

Comprehensive Patient Care

Comprehensive Patient Care

Tasaka-Fujita, Yuki Nishimura-Sakurai, Seishin Azuma, Yasuhiro Itsui, Mina Nakagawa, Minoru Tanabe,
Shinichi Takano, Mitsuharu Fukasawa, Minoru Sakamoto, Shinya Maekawa, Nobuyuki Enomoto, Mamoru
Watanabe. Comprehensive analyses of mutations and hepatitis B virus integration in hepatocellular
carcinoma with clinicopathological features. J. Gastroenterol. 2016.03; 51(5); 473-486
3. Miki Taniguchi, Megumi Tasaka-Fujita, Mina Nakagawa, Takako Watanabe, Fukiko Kawai-Kitahata,
Satoshi Otani, Fumio Goto, Hiroko Nagata, Shun Kaneko, Sayuri Nitta, Miyako Murakawa, Yuki NishimuraSakurai, Seishin Azuma, Yasuhiro Itsui, Kenichi Mori, Shintaro Yagi, Sei Kakinuma, Yasuhiro Asahina,
Mamoru Watanabe. Evaluation of Interferon Resistance in Newly Established Genotype 1b Hepatitis C
Virus Cell Culture System. J Clin Transl Hepatol. 2016.03; 4(1); 5-11
4. Seishin Azuma, Yasuhiro Asahina, Yuki Nishimura-Sakurai, Sei Kakinuma, Shun Kaneko, Hiroko Nagata,
Fumio Goto, Satoshi Ootani, Fukiko Kawai-Kitahata, Miki Taniguchi, Miyako Murakawa, Takako Watanabe, Megumi Tasaka-Fujita, Yasuhiro Itsui, Mina Nakagawa, Mamoru Watanabe. Eﬃcacy of additional
radiofrequency ablation after transcatheter arterial chemoembolization for intermediate hepatocellular
carcinoma. Hepatol Res. 2016.04; 46(4); 312-319
5. Shun Kaneko, Sei Kakinuma, Yasuhiro Asahina, Akihide Kamiya, Masato Miyoshi, Tomoyuki Tsunoda,
Sayuri Nitta, Yu Asano, Hiroko Nagata, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako Murakawa, Yasuhiro Itsui, Mina Nakagawa, Seishin Azuma, Hiromitsu Nakauchi, Hironori Nishitsuji, Saneyuki Ujino,
Kunitada Shimotohno, Masashi Iwamoto, Koichi Watashi, Takaji Wakita, Mamoru Watanabe. Human
induced pluripotent stem cell-derived hepatic cell lines as a new model for host interaction with hepatitis
B virus. Sci Rep. 2016.07; 6; 29358
6. Hiroko Nagata, Mina Nakagawa, Yuki Nishimura-Sakurai, Yu Asano, Tomoyuki Tsunoda, Masato Miyoshi,
Shun Kaneko, Fumio Goto, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako Murakawa, Sayuri Nitta,
Yasuhiro Itsui, Seishin Azuma, Sei Kakinuma, Naoko Tojo, Shuji Tohda, Yasuhiro Asahina, Mamoru
Watanabe, . Serial measurement of Wisteria ﬂoribunda agglutinin positive Mac-2-binding protein is
useful for predicting liver ﬁbrosis and the development of hepatocellular carcinoma in chronic hepatitis C
patients treated with IFN-based and IFN-free therapy.[ Epub ahead of print] Hepatol Int. 2016.07;
7. Fumio Goto, Sei Kakinuma, Masato Miyoshi, Tomoyuki Tsunoda, Shun Kaneko, Ayako Sato, Yu Asano,
Satoshi Otani, Seishin Azuma, Hiroko Nagata, Fukiko Kawai-Kitahata, Miyako Murakawa, Sayuri Nitta,
Yasuhiro Itsui, Mina Nakagawa, Yasuhiro Asahina, Mamoru Watanabe. Bone morphogenetic protein-4
modulates proliferation and terminal diﬀerentiation of fetal hepatic stem/progenitor cells. Hepatol. Res.
2016.09;
8. Miyako Murakawa, Yasuhiro Asahina, Fukiko Kawai-Kitahata, Mina Nakagawa, Sayuri Nitta, Satoshi
Otani, Hiroko Nagata, Shun Kaneko, Yu Asano, Tomoyuki Tsunoda, Masato Miyoshi, Yasuhiro Itsui,
Seishin Azuma, Sei Kakinuma, Yasuhito Tanaka, Sayuki Iijima, Kaoru Tsuchiya, Namiki Izumi, Shuji
Tohda, Mamoru Watanabe. Hepatic IFNL4 expression is associated with non-response to interferonbased therapy through the regulation of basal interferon-stimulated gene expression in chronic hepatitis
C patients. [ Epub ahead of print] J. Med. Virol. 2016.12;[Conference Activities & Talks]
1. Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyoshi M, Asano Y, Nagata H,
Goto F, Otani S, Kitahata F, Murakawa M , Itsui Y, Nakagawa M, Azuma S, Watanabe M. A Model forInteraction between HBV and Host Cells Derived from Human Induced Pluripotent Stem Cells. The 25th
Asian Paciﬁc Association for the Study of the Liver 2016.02.22 International Convention Center Pamir,Tokyo（JAPAN）
2. Nakagawa M. 【HCV 】Chair. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22
International Convention Center Pamir, Tokyo（JAPAN）
3. Kawai-Kitahata F, Asahina Y, Tanaka S, Kakinuma S, Murakawa M, Nitta S, Otani S, Goto F, Nagata
H, Kaneko S, Asano Y, Miyoshi M, Tsunoda T, Azuma S, Itsui Y, Nakagawa M, Tanabe M, Maekawa
S, Enomoto N, Watanabe M. Comprehensive analyses of mutations and hepatitis B virus integrationin hepatocellular carcinoma. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22
International Convention Center Pamir, Tokyo（JAPAN）― 209 ―

Comprehensive Patient Care

Comprehensive Patient Care

4. Nagata H, Sakurai Y, Nakagawa M, Kakinuma S, Azuma S, Murakawa M, Nitta S, Itsui Y, Asahina Y,
Watanabe M. Serial changes in M2BPGi levels as predictors of ﬁbrosis and HCC in chronic hepatitis C.
The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22 International Convention Center
Pamir, Tokyo（JAPAN）
5. Nakagawa M. 【HCV 11】Chair. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.23
International Convention Center Pamir, Tokyo（JAPAN）
6. Nakagawa M. 【HCV 12】Chair. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.23
International Convention Center Pamir, Tokyo（JAPAN）
7. Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyoshi M, Asano Y, Nagata H ,Goto F, Otani S, Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Watanabe M. A new model
for studying interaction between hepatitis B virus and host cells derived from human induced pluripotent
stem cells. EASL The International Liver Congress 2016 2016.04.14 Barcelona（Spain）
8. Jun Tsuruta, Kanako Noritake, Kouji Mizutani, Naoko Seki, Keiko Kondou, Shin-ichi Arakawa, Kouji
ARAKI. A Trial for a new IPW course for Dental students and Oral health sciences students working
in a dental team . Association of Dental Education in Europe, Annual Conference 2016.08.25 Balcelona
9. Jun Tsuruta, Kanako Noritake, Kouji Mizutani, Naoko Seki, Keiko Kondou, Shin-ichi Arakawa, Kouji
ARAKI. A new IPW course working in a dental team. 27th Annual meeting, Southeast Asia Association
for Dental Education 2016.09.08 Ho chi ming city
10. Jun Tsuruta. Implementing a new IPW course in the dental student clinic. The 5th Tri-University
Consortium on Oral Science and Education 2016.10.27 Peking, China
11. Shun Kaneko, Sei Kakinuma, Yasuhiro Asahina, Akihide Kamiya, Masato Miyoshi, Tomoyuki Tsunoda,
Sayuri Nitta, Ayako Sato, Yu Asano, Hiroko Nagata, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako
Murakawa, Yasuhiro Itsui, Mina Nakagawa, Seishin Azuma, Mamoru Watanabe. Genetically modiﬁed
human induced pluripotent stem cell-derived hepatic progenitor-like cell lines as a model for interaction
between hepatitis B virus and host cells. AASLD The Liver meeting 2016 2016.11.11 Boston（USA）[Awards & Honors]
1. Poster Presentation Award, 2016.09[Others]
1. Recognized Reviewer Status, 2016.02
As a reviewer; reviewing for Journal of Prosthodontic Research― 210 ―

Cognitive
Cognitive andand Behavioral
Behavioral Medicine
Medicine

Neuroanatomy and Cellular Neurobiology

Professor: TERADA Sumio
Assistant Professor: KAWAGISHI Masahiko
Assistant Professor: SAITO Kenta
Assistant Professor: SATO Keisuke
Graduate Student, MD-PhD Course, Research Fellow of the Japan Society for the Promotion of Science:
SATO Fumiya(until March)
Lab Manager, Administrative Assistant: TAGUCHI Mie(1)Research

Our lab has focused in two major directions:(1) How are cytoplasmic proteins transported in cells, and what other intracellular elements are necessary for
their quality control during transport?
How are the dynamics of cytoskeletal proteins in neurons regulated
and coordinated?
Neuronal cells such as neurons and glial cells are atypical and asymmetric in their morphology; both of themhaving long processes. They have to endure the burden of energy-consuming long-distance intracellular transport, and develop specialized cytoskeletal structures. Both intracellular transport and cytoskeletal dynamics
are inseparably interrelated, and essential for the cellular homeostasis and function. One of the main interestsof our laboratory is to understand how their dynamics are regulated and how these dynamics deﬁne neuronalmorphologies and functions.
(2) How do inhalation anesthetics exert their eﬀects on synaptic transmissions?
Our interests are in deciphering the long-lasting mystery of inhalation anesthetic eﬀects on synaptic transmissions, major mechanism in mammals that insures secure and painless surgical operations. We use electrophysiological preparations as well as newly developed spectroscopic techniques to identify their principles.
(2)

Education

Department of neuroanatomy and cellular neurobiology takes charge of basic neuroscience education for medical
undergraduate student (Lectures and Wet labs), especially from the morphological point of view.
For graduate school students, our group oﬀers introductory courses on both optical and electron microscopy(Lectures and Wet labs), with close relation to molecular and cellular neurobiology.
(3)

Publications[Original Articles]
1. Fumiya Sato, Hitoshi Asakawa, Takeshi Fukuma, Sumio Terada. Semi-in-situ atomic force microscopy
imaging of intracellular neuroﬁlaments under physiological conditions through the ’sandwich’ method.

― 211 ―

Cognitive andand Behavioral
Behavioral Medicine
Medicine
Cognitive

Microscopy (Oxf). 2016.08; 65(4); 316-324[Misc]
1. Kenta Saito, Masafumi Nakano,Takeharu Nagai. Luminescence Imaging: (a) Multicolor Visualization of
Ca2+ Dynamics in Diﬀerent Cellular Compartments and (b) Video-Rate Tumor Detection in a Freely
Moving Mouse. Meth. Mol. Biol.. 2016.01; 1461; 289-297[Conference Activities & Talks]
1. Terada S.. Cytoskeletal dynamics in neurons and beyond. Lecture 2016.01 China Soochow University
CAM-SU GRC
2. Terada S.. Close-up pictures of cytoskeletal dynamics in neurons. The 7th Asia Paciﬁc International
Congress of Anatomists 2016.03 Singapore
3. Masahiko Kawagishi, Yuki Obara, Takayuki Suzuki, Masumi Hayashi, Kazuhiko Misawa, Sumio Terada.
Direct Label-Free Measurement of the Distribution of Small Molecular Weight Compound Inside Thick
Biological Tissue using Coherent Raman Microspectroscopy. Biophysical Society 60th Annual Meeting2016.03.01 Los Angeles, California, USA― 212 ―

Cognitive
Cognitive andand Behavioral
Behavioral Medicine
Medicine

Systems Neurophysiology

Professor Izumi Sugihara
Associate Professor Yuriko Sugiuchi
Lecturer Yoshiko Izawa
Assistant Professor Mayu Takahashi
JSPS Postdoctoral Research Fellow Hermina Nedelescu
Students (dorcor) 6
Part-time lecturer Hirofumi Fujita(1)

OutlineDepartment of Systems Neurophysiology, formarly Department of Physiology #1 of the medical school, is oneof the basic medicine departments and take charge of research and education in the ﬁeld of neurophysiology
and related neurosciences.

(2)

Research

Our main interest lies in clarifying the structures that underlies function of the central nervous system andthen understanding their function. We are focused on the part of the central nervous system that is involvedin control of eye movements. The eye movement control system is located in the cerebrum, brainstem andcerebellum, has been studied in great detail and is important clinically. The cerebellum itself is another site offocus. Dysfunction of the cerebellum causes ataxia, a movement disorder associated with impaired control of
movement. We use electrophysiological, morphological and cell-biological approaches.
1) Cerebellar function
Distinct regions in the cerebellum make speciﬁc connections with diﬀerent areas of the brain and are involved in
the control of various movements including eye movements. For example, the neuronal circuitry that connects thelateral cerebrum, pontine nuclei, cerebellar cortex (hemisphere), cerebellar nucleus (dentate nucl.), thalamus andcerebrum is important for initiation, execution and control of movements. To understand cerebellar function, it
is important to understand the organization of the cerebellum into distinct anatomical regions, to characterize
the speciﬁc neuronal circuitry of these regions, and to identify how the cerebellum is organized into regions
and functions by way of the input and output systems. Our systematic approach to this question includes(developmental) anatomy, molecular biology, and electrophysiology. We have expertise in neuronal labeling withmarker molecules and tracers, single-axonal reconstruction, three-dimensional mapping of neuronal projection
patterns.

(3)

Education

We participate in Introductory Neurophysiology, Neuroscience and Physiology Lab courses for medical students(2nd year) as well as in courses for graduate students. We mainly teach the neurophysiology sections in thesecourses. Our goal is for students to understand normal function of nerve cells and the nervous system and,― 213 ―

Cognitive andand Behavioral
Behavioral Medicine
Medicine
Cognitiveon this ground, to understand pathological states of the nervous system in disease. For this purpose, we giveclinically-oriented lectures and laboratory courses linked with morphology and pharmacology.
(4)

Lectures & Courses

Our lectures cover transport and electric potential of the cell membrane, excitation and synaptic transmission(Introductory Neurophysiology), sensory systems, motor systems, autonomic nervous systems, and higher brain
function (Neuroscience), i.e. neurophysiology in general from the molecular, cellular through the organismic
levels. To promote students’ self-learning attitude, we sometimes employ an "active-learning" style. In thelaboratory course, we promote student-teacher discussion in small groups. We have had three "elective researchcourse" students.
(5)

Publications[Books etc]
1. Yuanjun Luo, Izumi Sugihara. The Olivocerebellar Tract. Springer, 2016 (ISBN : 978-3-319-24549-2)[Conference Activities & Talks]
1. Mayu Takahashi. Functional roles of commissural inhibitory and excitatory connections between the
superior colliculi for control of saccades in relation to the VOR system and Listing s law. Dissecting andUnderstanding Mechanisms for Functional Shift of Brain Network 2016.03.03
2. Sugiuchi Y, Takahashi M, and Shinoda Y.. Supranuclear pathways for vertical saccade compared withthose for horizontal saccade and vestibuloocular reﬂex. . The 29th Barany Society Meeting 2016 2016.06.05
Seoul, Korea.
3. Yoshiko Izawa, Hisao Suzuki. Activity of frontal eye ﬁeld ﬁxation neurons and its relation to the suppression of saccades and smooth pursuit. The 29th Barany Society Meeting 2016 2016.06.06 Seoul, Korea
4. Mayu TAKAHASHI. TECTAL COMMISSURAL CONNECTIONS AND THEIR FUNCTIONAL ROLEOF VERTICAL SACCADES IN RELATION TO LISTING’S LAW AND VOR. 29th Barany Society
Meeting 2016 2016.06.06
5. Mayu Takahashi. Neural circuit of the superior collisulus output system for control of coordinated eye
and head movements. The 39th Annual Meeting of the Japan Neuroscience Society 2016.07.22― 214 ―

Cognitive
Cognitive andand Behavioral
Behavioral Medicine
Medicine

Pharmacology and Neurobiology

Professor:Tsutomu TANABE
Assistant professor:Hironao SAEGUSA
Assistant professor:Makoto FUJIKAWA
Assistant professor:Daisuke TANAKA(1)

OutlineMany intriguing mysteries left in the issue of brain function like (1) learning and memory, (2) cognition andbehavior, (3) generation of consciousness, (4) personality and mentality. On the other hand, in the modernday world with a complicated human relations and prolonged life span, necessity of deeper understanding anddevelopment of the means to cure the numerous neurological disorders and pain is enormously increased.

(2)

Research

1. Regulation of Microglial function in Neuroinﬂammation/Neurodegenerative diseases
2. Regulation of Macrophage function in Inﬂammatory bowel disease and Rheumatoid arthritis
3. Energy metabolic imaging at single cell level of cancer stem cell/cancer cell using Bioluminescence and FRET
and Imaging
4. Energy metabolic imaging at single cell level of neuron, microglia and astrocyte in the degenerative area ofthe mouse model of various neurodegenerative diseases
5. Neural mechanisms of pleasure and motivation in feeding
6. Molecular basis of Calcium channelopathy
7. Alteration of Neuron-Glia interaction in Neurological disorders(3)

Education

Undergraduate course: Pharmacology course provides the principle of pharmacological basis of therapeutics.
Several representative therapeutic drugs in each disease will be picked up and systematic lectures -from basic
pharmacology to mechanism of action, drug metabolism, clinical application and side eﬀects- will be provided.
Students are projected to acquire self-learning skills during the course and expected to be ready for handlingclinical cases by pharmacological means.
We consider education through the pharmacology lab work is important. Students are given opportunity todissect out several tissues (heart, skeletal muscle, ileum and vas deferens) from living animals by themselves
and test the eﬀect of a number of drugs including speciﬁc agonist, antagonist and non-selective drugs. Lab
work course is divided into two parts. In the ﬁrst part, students were given several known drugs for testing
the known eﬀect on these tissues. In the second part, students are given two unknown drugs and requested to
identify the name and concentration of each drug using the tissues they prepare by themselves.
Graduate course: During the ﬁrst couple of months, students are requested to acquire basic techniques ofbiochemistry, molecular biology, pharmacology and electrophysiology that are routinely used in our laboratory.
Then students will be given a small project to do using the techniques they have learned during the initial― 215 ―

Cognitive andand Behavioral
Behavioral Medicine
Medicine
Cognitive

training. Students are also required to read relevant scientiﬁc papers and conduct seminar style lectures toother lab members monthly. After completion of the initial phase, students start their own project under thesupervision of the faculties in the lab.

― 216 ―

Cognitive
Cognitive andand Behavioral
Behavioral Medicine
Medicine

Molecular Neuroscience

Professor Kohichi Tanaka
Associate Professor Tomomi Aida
Assistant Professor Saeko Ishida
Assistant Professor Yuichi Hiraoka (2015/5/1 )Graduate Student (doctor course)
Zhao Zhuoyang
Kaori Sugiyama
Takehisa Handa
Graduate Student (master course)
Mina Kusunose
Moeko Tanaka
Haruka Takigawa
Hiroshi Ogawa
Technical Staﬀ
Harumi Ishikubo
Secretary
Satomi Ohno
(1)

OutlineThe ﬁnal goal of our research is to understand molecular, cellular, and neuronal ensemble mechanisms underlying
higher order brain functions including learning and memory. For that purpose, we combine molecular genetics,
physiological and behavioral methods. The laboratory also studies the mechanism that underlies neuronal cell
death and regeneration.
(2)

Research

1. Functions of glutamate transporters in the brain
Glutamate is a major excitatory neurotransmitter and plays an important role in neuronal plasticity and neurotoxicity in the central nervous system. Glutamate transport proteins provide the mechanism by which synaptically released glutamate is inactivated and kept below toxic levels in the extracellular space. By now, ﬁve
subtypes of high-aﬃnity glutamate transporters have been identiﬁed in the mammalian brain. Our lab studies
the physiological and pathological roles of glutamate transporter subtypes using subtype-speciﬁc knockout mice.
Clinical phenotypes of spinocerebellar ataxia type-5 (SCA5) and spectrin-associated autosomal recessive cerebellar ataxia type-1 (SPARCA1) are mirrored in mice lacking β-III spectrin (β-III-/-). One function of β-III
spectrin is the stabilization of the Purkinje cell-speciﬁc glutamate transporter EAAT4 at the plasma membrane.
In β-III-/- mice EAAT4 levels are reduced from an early age. In contrast levels of the glutamate transporter
GLAST, expressed in Bergmann glia, only fall progressively from 3 months onwards. Here we elucidated theroles of these two glutamate transporters in cerebellar pathogenesis mediated through loss of β-III spectrin
function by studying EAAT4 and GLAST knockout mice as well as crosses of both with β-III-/- mice. Our― 217 ―

Cognitive andand Behavioral
Behavioral Medicine
Medicine
Cognitive

data demonstrate that EAAT4 loss, but not abnormal AMPA receptor composition, in young β-III-/- mice
underlies early Purkinje cell hyper-excitability and that subsequent loss of GLAST, superimposed on the earlier
deﬁciency of EAAT4, is responsible for Purkinje cell loss and progression of motor deﬁcits. Yet the loss of
GLAST appears to be independent of EAAT4 loss, highlighting that other aspects of Purkinje cell dysfunction
underpin the pathogenic loss of GLAST. Finally, our results demonstrate that Purkinje cells in the posterior
cerebellum of β-III-/- mice are most susceptible to the combined loss of EAAT4 and GLAST, with degenerationof proximal dendrites, the site of climbing ﬁbre innervation, most pronounced. This highlights the necessity foreﬃcient glutamate clearance from these regions and identiﬁes dysregulation of glutamatergic neurotransmission
particularly within the posterior cerebellum as a key mechanism in SCA5 and SPARCA1 pathogenesis.
We investigated the cytoprotective eﬀect of geranylgeranylacetone (GGA) on RGCs degeneration using a normal
tension glaucoma (NTG) mouse model, which lacks GLAST. Three-week-old GLAST+/− mice were given oral
administration of GGA at 100, 300, or 600 mg/kg/day or vehicle alone, and littermate control mice were given
vehicle alone for 14 days, respectively. The number of RGCs of GLAST+/− mice signiﬁcantly decreased, as
compared to that of control mice. RGC loss was signiﬁcantly suppressed by administration of GGA at 600
mg/kg/day, compared with vehicle alone. Following GGA administration, HSP70 was signiﬁcantly upregulated
together with reduction in the activities of caspase-9 and -3. Our studies highlight HSP70 induction in theretina is available to suppress RGC degeneration, and thus GGA may be applicable for NTG as a promisingtherapy.
2. Development of genome editing technologies
Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing longhomology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Casbased precise integration into the target chromosome (PITCh) system for a gene cassette knock-in without longhomology arms mediated by microhomology-mediated end-joining.
Here, we identiﬁed exonuclease 1 (Exo1) as an enhancer for PITCh in human cells. By combining the Exo1
and PITCh-directed donor vectors, we achieved convenient one-step knock-in of gene cassettes and ﬂoxed allele
both in human cells and mouse zygotes. Our results provide a technical platform for high-throughput knock-in.

(3)

Education

Goals/Outline:
Students should generate genetically modiﬁed animals to comprehensively understand the cognitive mechanisms
at the level of molecule to behavior. Then, students should analyze cognitive deﬁcits of mutant animals andthose molecular mechanisms.
Available programs:
Participation in the ongoing research project; as needed
Training for cell biology: ﬁve times a year 13:00 – 16:00
Experiment:
1. Gene cloning and generation of targeting vector.
2. Generation of genetically modiﬁed mice
3. Behavioral analysis of the mice
4. Morphological analysis of central nervous systems.

(4)

Lectures & Courses

The aim of this practice is to learn molecular biological, anatomical, electrophysiological and psychologicalapproaches to elucidate the mechanism of cognition. Moreover, based on previous case reports of cognitivedeﬁcits, students should plan and discuss what kinds of the researches are possible and meaningful to elucidate
the pathology of these diseases, leading to unveil the mechanism of cognition.

― 218 ―

Cognitive
Cognitive andand Behavioral
Behavioral Medicine
Medicine(5)

Publications[Original Articles]
1. Shinji Miyazaki, Yuichi Hiraoka, Shizu Hidema, Katsuhiko Nishimori. Prenatal minocycline treatment
alters synaptic protein expression, and rescues reduced mother call rate in oxytocin receptor-knockout
mice Biochemical and Biophysical Research Communications. 2016.02; Volume 472( Issue 2); 319-323
2. Elise Marsan, Saeko Ishida, Adrien Schramm, Sarah Weckhuysen, Giuseppe Muraca, Sarah Lecas, Ning
Liang, Caroline Treins, Mario Pende, Delphine Roussel, Michel Le Van Quyen, Tomoji Mashimo, Takehito
Kaneko, Takashi Yamamoto, Tetsushi Sakuma, Séverine Mahon, Richard Miles, Eric Leguern, Stéphane
Charpier, Stéphanie Baulac. Depdc5 knockout rat: A novel model of mTORopathy. Neurobiol. Dis..
2016.05; 89; 180-189
3. Shizu Hidema, Tomokazu Fukuda, Yuichi Hiraoka, Hiroaki Mizukami, Ryotaro Hayashi, Ayano Otsuka,
Shingo Suzuki, Shinji Miyazaki, and Katsuhiko Nishimori. Generation of Oxtr cDNA(HA) -Ires-Cre Micefor Gene Expression in an Oxytocin Receptor Speciﬁc Manner. Journal of Cellular Biochemistry. 2016.05;
Volume 117, (Number 5 ); 1099-1111
4. Emma M Perkins, Daumante Suminaite, Yvonne L Clarkson, Sin Kwan Lee, Alastair R Lyndon, Jeﬀrey
D Rothstein, David J A Wyllie, Kohichi Tanaka, Mandy Jackson. Posterior cerebellar Purkinje cells in an
SCA5/SPARCA1 mouse model are especially vulnerable to the synergistic eﬀect of loss of β-III spectrin
and GLAST. Hum. Mol. Genet.. 2016.10; 25(20); 4448-4461
5. Zhenyu Dong, Yasuhiro Shinmei, Yoko Dong, Saori Inafuku, Junichi Fukuhara, Ryo Ando, Nobuyoshi
Kitaichi, Atsuhiro Kanda, Kohichi Tanaka, Kousuke Noda, Takayuki Harada, Shinki Chin, Susumu Ishida.
Eﬀect of geranylgeranylacetone on the protection of retinal ganglion cells in a mouse model of normal
tension glaucoma. Heliyon. 2016.10; 2(10); e00191
6. Tomomi Aida, Shota Nakade, Tetsushi Sakuma, Yayoi Izu, Ayu Oishi, Keiji Mochida, Harumi Ishikubo,
Takako Usami, Hidenori Aizawa, Takashi Yamamoto, Kohichi Tanaka. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ. BMC Genomics. 2016.11; 17(1); 979― 219 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

Neuropathology

Professor: Hitoshi Okazawa
Practical professor: Kazuhiko Tagawa
Project Lecturer/Part-time Lecturer: Haruhisa Inoue, Masaki Sone, Toshiki Uchihara
Project assistant lecturer: Hiroshi Tsuda
Assistant professor: Kyota Fujita
Project Assistant professor: Xigui Chen, Hidenori Homma, Kazumi Motoki, Emiko Yamanishi
Graduate Student：Mao Ying、Juliana Bosso Taniguchi、Eriko Hoshino、Hikari Tanaka、Tomohiro Kumaki(1)

OutlineThe goals of our research are to elucidate molecular mechanisms of neurodegenerative disorders as well as ofmental retardation, and to develop novel therapeutics for those intractable diseases. In neurodegeneration,
we are now focusing on polyglutamine diseases including hereditary spinocerebellar degenerations and Huntington’s disease. Knowledge from transcriptome and proteome analyses of the pathologies will lead to new
types of molecular therapeutics. In reference to mental retardation, we are developing animal models andanalyzing molecular pathologies of our original molecule PQBP1 whose mutations cause mental retardation
with microcephaly. This line of research is also for developing new therapeutics of the common but intractablediseases.
(2)

ResearchResearch Contents
Following studies have been intensively carried out in our laboratory.
1) Investigation of molecular pathologies of neurodegenerative diseases.
2) Studies on impairment of DNA-repair in polyglutamine diseases.
3) Development of new seed drugs for neurodegeneration.
4) Development of new seed drug for mental retardation.
5) Investigation of molecular functions of Oct-3/4
Below is the brief report of this year s progress.
1. Gene therapy for Spinocerebellar Ataxia model mice using AAV-RpA1 vector
Spinocerebellar ataxia type-1 (SCA1) is an intractable neurological disease caused by CAG repeat expansion of
the Ataxin-1 (Atxn1) gene. This mutant Atxn-1 induces dysfunctions and neurodegeneration mainly of cerebellar Purkinje cells and spinal motor neurons. We previously reported that DNA damage repair is one of thedomain of SCA1 molecular pathology. Comprehensive proteome analysis of soluble nuclear proteins indicatedthat HMGB1/2 were decreased in neurons expressing mutant Atxn1 in comparison to those expressing normal
Atxn1 (Qi et al., 2007). We also showed that mitochondrial HMGB1 was decreased in mutant Atxn1-KI mice
(Ito et al., 2015). Mutant Atxn1 increased DNA damage, which were rescued by supplementation of HMGB1(Qi et al., 2007; Ito et al., 2015).
RpA1 is a critical molecule involved in multiple type of DNA damage repair system. We previously revealed
that RpA1 was another target molecule of DNA damage repair in SCA1 that was expected from gene screensin Drosophila models. In this study, we tested gene therapy of mutant Atxn1-KI mice with adeno-associated― 220 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

virus (AAV) vector expressing RpA1.
AAV-RpA1 vector was injected into the surface of the cerebellum (subarachnoid space). We found signiﬁcanteﬀects on motor functions, normalization of DNA damage markers, and improved Purkinje cell morphology.
Surprisingly, such eﬀects lasted for 50 weeks following AAV-RpA1 infection. We also conﬁrmed that AAV-RpA1
indirectly recovered multiple cellular functions such as RNA splicing, transcription and cell cycle as well as abnormal morphology of dendrite and dendritic spine of Purkinje cells in Atxn1-KI mice.
2. Strategy of molecular target for pathological signaling before aggregation of beta-Amyloid.
Pathological feature of neurodegenerative disease such as Alzheimer s disease is abnormal protein aggregation
inside or outside of cells. In Alzheimer s disease, senile plaque composed of beta-Amyloid and neuroﬁbrillarytangle composed of tau. Based on amyloid hypothesis, clinical trials have been challenged with the aim of clearance of extracellular amyloid aggregation. However, antibody therapy against beta-Amyloid did not succeed
the recovery of cognitive decline although it could reduce the amyloid plaque. Therefore, our recent researchinterest is focusing on the earlier interpretation of antibody treatment before aggregation of beta-Amyloid, or
new molecular target therapy based on early-phase (phase 0) change of pathological signaling.
Previously we performed comprehensive phosphoproteome analysis in order to identify the phase 0 molecular
events, and we identiﬁed 17 core-protein phosphorylation changed from early-phase to late-phase, and among
them, 3 phosphoprotein were changed at phase 0. MARCKS, one of these proteins, is known as a substrate of
PKC, was phosphorylated not only PKC-phosphorylation site but also other phosphorylation site modiﬁed byanother kinases. Thus, in the present study, we compared the change of molecular pattern in model mice andhuman postmortem brain in order to detect the key phosphorylation site. We found that Ser46 were phosphorylated at early-phase, and antibody against pSer46-MARCKS depicted degenerative neuritis surrounded theamyloid plaques. We also found the pSer46-MARCKS lost its ability to bind cytoskeletal protein, actin, andfailed to maintain the spine. Phosphorylation of MARCKS at ser46 was due to HMGB1 (one of the DAMPS;
damage-associated molecular pattern), not beta-Amyloid. Also, HMGB1 in spinal ﬂuid of patients with earlyprogression was shown higher than others, which suggest that pSer46-MARCKS is closely related to the pathology of Alzheimer s disease in human patients. Finally, we tried the rescue experiments using neutralizing
antibody against HMGB1, and we succeeded the decrease of pSer46-MARCKS, rescued the reduction of spine,
and improved the cognitive decline in model mice. HMGB1 can be released from not only leakage from deadcells but also hyperactive living neurons. Therefore, antibody therapy targeting HMGB1 have a potential topathology of phase 0 before aggregation of beta-amyloid, and it might be beneﬁcial for prevention of AD pathogenesis.
3. New therapeutic strategy for Huntington s disease: targeting the third atypical cell death.
The common important feature of neurodegenerative diseases, such as Alzheimer s disease, Parkinson s disease,
Huntington s disease, Spinocerebellar Ataxia, Amyotrophic lateral sclerosis, is slow-progressive neurodegeneration over the decades. Such slow-progressive dysfunction of neuronal cells and their cell death have diﬀerent
aspects from other neurological disorders such as cerebral infarction or cerebral hemorrhage, which symptoms
have been completed within a few minutes or a few hours. The characteristics of cell death in neurodegenerative
disease have been greatly debated. The typical types of cell death are apoptosis and necrosis, but there are othertypes of cell death such as autophagy, necroptosis, and TRIAD (transcriptional repression-induced atypical cell
death). We previously identiﬁed TRIAD, which can be triggered by speciﬁc inhibition of RNA polymerase II
and can be executed slow-progressively. The morphological characteristics of TRIAD are diﬀerent from thatof apoptosis or autophagy, but key morphology is abnormal expansion of endoplasmic reticulum (ER). Also,
we previously reported that YAP, a candidate molecule obtained through comprehensive expression analysis,might be involved in signal cascade of TRIAD execution. In the present studies, we ﬁrst performed screeningof TRIAD-related molecules associated with several types of cell death, then mixed with bioinformatics bymatching the data with protein-protein interaction database in order to comprehensive search for identiﬁcationof signal network of TRIAD. Then, we found hnRNP, a RNA-binding molecule, and Htt (causative gene for
Huntington s disease) are related to TRIAD (Mao et al., 2016a)
On the other hand, we found that mutant-Htt-expressing neurons showed not apoptotic or necrotic cell deathbut cell death with asymmetric cytoplasmic expansion. This expansion was endoplasmic reticulum expansion,and by two-photon microscopic analysis, we revealed that similar expansion of ER could be seen in living model
mice brain carrying mutant Htt (Mao et al., 2016b).
We also tried to understand the molecular mechanism under the execution of TRIAD, and we found that loss of
YAP-TEAD dependent survival signal is mainly contributed. Especially activation of Hippo-pathway prevented
the intranuclear translocation of YAP, and phosphorylation of YAP by Plk1 increased binding of YAP/p73, also

― 221 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

mutant Htt bounded to and incorporated YAP into its inclusion, which all leads to the loss of survival signaling(ﬁgure C in Highlight ). Finally, we treated S1P to mutant Htt Knockin mice in order to promote YAPtranslocation to nucleus and prevent Hippo-pathway activation, and we succeeded to improve their behavioral
impairment and the abnormal control of ER size. What is more, we found ER expansion in human HD patientsby electron microscopy. We also conﬁrmed the biochemical and histological alteration of hippo-pathway in human HD patients as well as HD model mice（Yamanishi et al., 2017）. Collectively, our present studies suggestthat treatment targeting signaling pathway of TRIAD is beneﬁcial for HD pathology.
4. Identiﬁcation of candidate seed drugs for Huntington s disease by integrated screens of chemical libraries.
We previously reported the pathological function of Ku70 in Huntington s disease. It is a key molecule for
non-homologous end-joining (NHEJ), a type of DNA double-strand break repair that functions in non-dividing
cells such as diﬀerentiated neurons. By protein-protein interaction screenings, we identiﬁed Ku70 as moleculethat directly interacts with Htt (Enokido et al, 2010). Furthermore, transgenic overexpression of Ku70 resulted
in one of the longest lifespan extensions in HD model mice.
Given such previous results have suggested the importance of Ku70 relative to various mediator molecules,
we screened chemicals that could inhibit the interaction between Ku70 and mutant Htt. We employed both
wet and dry screening (MF20 and Discovery Studio, respectively) for the initial step, performed a secondary
screening with single molecule ﬂuorescence spectroscopy, and examined the phenotypic eﬀects in Drosophila andmouse models as the third and fourth screens. Then we found that two chemicals exerted an in vivo eﬀect in amouse HD model such as lifespan extension, body weight recovery, motor function recovery, and DNA damage
repair. Unexpectedly, the selected chemicals enhanced rather than inhibited Htt aggregation, as indicated bydynamic light scattering analysis. This may provide additional insight about whether polyQ aggregates are
toxic, protective or both.

(3)

Education

As educational tasks, we have lecture and experiment classes of neuropathology for medical/dental graduate
school program and medical school program. We also have general pathology and neuropathology classes forgraduate school for health sciences, and clinical anatomical and therapeutic pathology classes for research
students.

(4)

Lectures & Courses

We provide students with opportunities to learn practical research techniques on neuropathology, especiallynerurodegenerative diseases.
(5)

Publications[Original Articles]
1. Mao, Y., Tamura, T., Yuki, Y., Abe, D., Tamada, Y., Imoto, S., Tanaka, H., Homma, H., Tagawa, K.,
Miyano, S., and Okazawa, H. The hnRNP-Htt axis regulates necrotic cell death induced by transcriptional
repression through impaired RNA splicing. Cell Death & Disease. 2016.04; 7; e2207
2. Mizuguchi, M., Obita, T., Kajiyama, A., Kozakai, Y., Nakai, T., Nabeshima, Y. and Okazawa, H..
Allosteric modulation of the binding aﬃnity between PQBP1 and the spliceosomal protein U5-15kD.
FEBS Lett.. 2016.06; 590(14); 2221-2231
3. Taniguchi, JB., Kondo, K., Fujita, K., Chen, X., Homma, H., Sudo, T., Mao, Y., Watase, K., Tanaka, T.,
Tagawa, K., Tamura, T., Muramatsu, SI., Okazawa, H.. RpA1 ameliorates symptoms of mutant Ataxin-1
knock-in mice and enhances DNA damage repair. Hum Mol Genet.. 2016.08;
4. Fujita, K., Motoki, K., Tagawa, K., Chen, X., Hama, H., Nakajima, K., Homma, H., Tamura, T., Watanabe, H., Katsuno, M., Matsumi, C., Kajikawa, M., Saito, T., Saido, T., Sobue, G., Miyawaki, A., Okazawa,
H.. HMGB1, a pathogenic molecule that induces neurite degeneration via TLR4-MARCKS, is a potential
therapeutic target for Alzheimer’s disease. Scientiﬁc Reports.. 2016.08; 6; 31895― 222 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

5. Mao, Y., Chen, X., Xu, M.,Fujita, K., Sasabe, K.,Homma, H., Murata, M., Tagawa, K., Tamura, T., Kaye,J., Finkbeiner, S., Blandino, G., Sudol, M., Okazawa, H. . Targeting TEAD/YAP-transcription-dependentnecrosis, TRIAD, ameliorates Huntington s disease pathology. Hum Mol Genet.. 2016.09;
6. Imamura, T., Fujita, K., Tagawa, K., Ikura, T., Chen, X., Homma, H., Tamura, T., Mao, Y., Taniguchi,
JB., Motoki, K., Nakabayashi, M., Ito, N., Yamada, K., Tomii, K., Okano, H., Kaye, J., Finkbeiner, S.,
Okazawa, H.. Identiﬁcation of hepta-histidine as a candidate drug for Huntington s disease by in silico-in
vitro-in vivo-integrated screens of chemical libraries. Scientiﬁc Reports.. 2016.09; 6; 33861
7. Mao, Y., Chen, X., Xu, M.,Fujita, K., Sasabe, K.,Homma, H., Murata, M., Tagawa, K., Tamura, T., Kaye,J., Finkbeiner, S., Blandino, G., Sudol, M., Okazawa, H. . Targeting TEAD/YAP-transcription-dependentnecrosis, TRIAD, ameliorates Huntington s disease pathology. Hum Mol Genet.. 2016.09;[Conference Activities & Talks]
1. Tamura T, Shiraishi R, Sone M, Okazawa H. Systematic Analysis of Fly Models Elucidates Sleep disturbance in Huntington’s Disease. The 57th Annual Meeting of the Japanese Society of Neurology 2016.05.18
Kobe International Exhibition Hall (Kobe)
2. Ito H, Fujita K, Chen X, Okazawa H. Analysis of molecular mechanism and development of therapeutic
methods in spinocerebellar ataxia type 1 (SCA1). The 57th Annual Meeting of the Japanese Society of
Neurology 2016.05.18 Kobe International Exhibition Hall (Kobe)
3. Takuya Tamura, Risa Shiraishi, Masaki Sone, Hitoshi Okazawa. Systematic Analysis of Fly Models
Elucidates Sleep disturbance in Huntington’s Disease. The 57th Annual Meeting of the Japanese Societyof Neurology 2016.05.18 Kobe International Exhibition Hall (Kobe)
4. Hikaru Ito, Kyota Fujita, Xigui Chen, Hitoshi Okazawa. Analysis of molecular mechanism and development of therapeutic methods in spinocerebellar ataxia type 1 (SCA1). The 57th Annual Meeting of theJapanese Society of Neurology 2016.05.18 Kobe International Exhibition Hall (Kobe)
5. Chen X, Kondo K, Motoki K, Homma H, Okazawa H. Fasting activates macroautophagy in neurons ofAD mice but is insuﬃcient to degrade amyloid-beta. The 57th Annual Meeting of the Japanese Societyof Neurology 2016.05.19 Kobe International Exhibition Hall (Kobe)
6. Mao Y, Tamura T, Yuki Y, Abe D, Tamada Y, Imoto S, Tanaka H, Homma H, Tagawa K, Miyano
S, Okazawa H. The hnRNP-Htt axis regulates necrotic cell death induced by transcriptional repression
through impaired RNA splicing. The 39th Annual Meeting of the Japan Neuroscience Society 2016.07.20
Paciﬁco Yokohama (Yokohama)
7. Okazawa H. Gene therapy to conquer spinocerebellar ataxia. The 22nd Annual Meeting of Japan Societyof Gene and Cell Therapy 2016.07.30 Toranomon Hills Forum (Tokyo)
8. Mizuguchi M, Nabeshima Y, Obita T, Okazawa H. Interaction of spliceosomal proteins PQBP1, U515kD, and WBP11. The XXVIIth International Conference on Magnetic Resonance in Biological Systems
2016.08.22 International Conference Center Kyoto (Kyoto)
9. Furotani K, Yajima T, Toyokawa M, Nakayama M, Tamura T, Okazawa H, Sone M. Eﬀect of inhibitionof synaptic delivery of APP by loss-of-function of yata for the Drosophila Alzheimer’s disease model. The
12th Japanese Drosophila Research Conference 2016.09.09 Rikkyo University (Tokyo)
10. Okazawa H. Therapeutics development of Alzheimer s disease targeting on Phase 0. 15th SurugadaiInternational Symposium 2016.11.29 Tokyo Medical and Dental University (Tokyo)
11. Furotani K, Yajima T, Toyokawa M, Nakayama M, Tamura T, Okazawa H, Sone M. Eﬀect of inhibitionof synaptic delivery of APP by loss-of-function of yata for the Drosophila Alzheimer’s disease model. The
39th Annual Meeting of the Molecular Biology Society of Japan 2016.12.01 Rikkyo University (Tokyo)― 223 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

Ophthalmology and Visual Science

Professor;Kyoko Ohno-Matsui
Specially-appointed professor;Makoto Aihara
Junior Associate Professor;Hiroshi Takase, Koju Kamoi, Takeshi Yoshida
Assistant Professor;Shintaro Horie, Kei Morohoshi, Tae Yokoi
Graduate student;Tomoka Ishida, Yuko Iwasaki, Liu hongcling, keijia Cao, Yuxin Fang
(1)

OutlineOur department was established in 1944. Prof. Jin Ohtsuka initiated research on myopia in 1946, and Emeritus
Prof. Takashi Tokoro established high myopia clinic in 1974 as the world only clinic speciﬁc to pathologic
myopia. To date, clinical practice as well as basic research on myopia have continuously been performed in our
department. Uveitis clinic was established by Emeritus Prof. Manabu Mochizuki in 1988. Since Prof. Kyoko
Ohno-Matsui was appointed to a professorship in our department, clinical practice and basic research on wide
variety of ﬁelds such as glaucoma, cataract, diabetic retinopathy, vitreoretinal disorder, and macular diseases
in addition to myopia and uveitis have been actively performed.
(2)

Research

1. High myopia
1) Analysis of retinochoroidal complications in high myopia (choroidal neovascularization, myopic tractional
retinopathy)
2) Evaluation of the molecular mechanism of choroidal angiogenesis using the cultured cells as well as experimental animals (collaboratory project with Department of Cellular Physiological Chemistry)3) Gene analysis of highly myopic patients (collaborator project with Kyoto University)4) Establishment of a novel therapy to prevent an axial elongation or the formation of posterior staphyloma
2. Ocular immunology and inﬂammation
1) Evaluation of the molecular mechanism of immunoregulartion in intraocular inﬂammation
2) Pathogenic mechanism of intraocular inﬂammatory diseases
3) Development of novel treatments of intraocular inﬂammation
4) Molecular diagnosis of virus-infected uveitis and intraocular lymphomas.
3. Neuro-ophthalmology
1) Evaluation of the change of the circulation as well as the glucose metabolism in the visual cortex using
positron emission tomography (PET) in various ocular disorders
2) Mechanism of visual pathway in normal conditions as well as in the patients with amblyopia.
4. Vitreoretinal disorder1) Development of a novel treatment for vitreoretinal disorders like retinal detachment, diabetic retinopathy,
and macular holes.
5. Strabismus and amblyopia clinic
1) Eﬀect of the visual background on binocular vision as well as the inﬂuence of strabismus on dynamic visualacuity.

― 224 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine(3)

Education

Undergraduate education of ophthalmology is composed of 1) classes on histology and physiology of the eye, andon diagnosis and treatment of ocular disorders, 2) combination block in which clinical examination is trained,
and the diagnostic process is actively learned through group discussion using case series, 3) pre-clerkship andclerkship in which the medical students practically learn the major ocular disorders by seeing the patients anddiscussing in the conference.
After the initial residency of the ﬁrst two years, the residency in ophthalmology in programmed for four years
according to the educational program on diploma of ophthalmology by Japanese ophthalmological society.
The graduate students are expected to be academic doctors who develop and perform highly-qualiﬁed ophthalmologists, as well as become scientists who can perform basic research focusing on their clinical interest.
(4)

Lectures & Courses

Main objective of ophthalmology and visual science in the graduate course is to obtain the highly-advanced
knowledge in the diagnosis and the treatment of various ocular disorders and to perform the basic researchbased on clinical experience.
(5)

Clinical Services & Other Works

Clinical practice is organized by the general ophthalmology clinic as well as the several subspecialty clinics.
When the patients visited our department, they are screened in the general clinic, and then the ﬁnal decision of
the diagnosis and treatment is made in cooperation with each subspecialty clinic. Subspecialty clinics include
high myopia clinic, uveitis clinic, glaucoma clinic, vitreoretinal disorder clinic, diabetic retinopathy clinic, neuroophthalmology clinic, and medical retina clinic. Approximately, 1,300 surgeries are performed per year (e.g.,cataract surgery, vitreoretinal surgery, glaucoma surgery, strabismus surgery).
(6)

Publications[Original Articles]
1. Koju Kamoi, Yoichi Nagata, Manabu Mochizuki, Daisuke Kobayashi, Nobuhiro Ohno, Kaoru Uchimaru,
Arinobu Tojo, Kyoko Ohno-Matsui.. Formation of Segmental Rounded Nodules During Inﬁltration of
Adult T-Cell Leukemia Cells Into the Ocular Mucous Membrane. Cornea. 2016.01; 35(1); 137-139
2. Kamoi K, Nagata Y, Mochizuki M, Kobayashi D, Ohno N, Uchimaru K, Tojo A, Ohno-Matsui K. Formation of segmental rounded nodules during the inﬁltration of adult T-cell leukemia cells into ocular mucous
membrane. Cornea. 2016.01; 35(1); 137-139
3. Higashide T, Ohkubo S, Hangai M, Ito Y, Shimada N, Ohno-Matsui K, Terasaki H, Sugiyama K, Chew P,
Li KK, Yoshimura N. Inﬂuence of clinical factors and magniﬁcation correction on normal thickness proﬁlesof macular retinal layers using optical coherence tomography. PLoS ONE. 2016.01; 11(1); e0147782
4. Ohno-Matsui K, Jonas JB, Spaide RF. Macular bruch membrane holes in choroidal neovascularizationrelated myopic macular atrophy by swept-source optical coherence tomography. Am. J. Ophthalmol..
2016.02; 162; 133-139.e1
5. Koh V, Tan C, Tan PT, Tan M, Balla V, Nah G, Ching-Yu C, Ohno-Matsui K, Tan MM, Yang A, Zhao
P, Wong TY, Seang-Mei S. Myopic maculopathy and optic disc changes in highly-myopic young Asianeyes and impact on visual acuity. Am. J. Ophthalmol.. 2016.04; 164; 69-79
6. Jonas JB, Xu L, Wang YX, Bi HS, Wu JF, Jiang WJ, Nangia V, Sinha A, Zhu D, Tao Y, Guo Y, You QS,
Wu LJ, Tao LX, Guo XH, Ohno-Matsui K, Panda-Jonas S. Education-related parameters in high myopia:
Adults versus school children. PLoS ONE. 2016.05; 11(5); e0154554
7. Kimura Y, Akagi T, Miyake M, Yamashiro K, Yoshikawa M, Yamada H, Hasegawa T, Suda K, Nakanishi
H, Ohashi-Ikeda H, Gotoh N, Hangai M, Moriyama M, Ohno-Matsui K, Yoshimura N. Association between the CDKN2B-AS1 gene and primary open angle glaucoma with high myopia in japanese patients.
Ophthalmic Genet. 2016.06; 37(2); 242-244― 225 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

8. Ohno-Matsui K, Jonas JB, Spaide RF. Macular bruch’s membrane holes in highly myopic patchy chorioretinal atrophy. Am. J. Ophthalmol.. 2016.06; 166; 22-28
9. Holz FG, Tufail A, Leveziel N, Lai TY, Lanzetta P, Wong TY, Yu HG, Chen YX, Heinrichs N, Pilz S,
Ohno-Matsui K. Ranibizumab in myopic choroidal neovascularization: A subgroup analysis by ethnicity,age, and ocular characteristics in RADIANCE. Ophthalmologica. 2016.06; 236(1); 19-28
10. Shinohara K, Moriyama M, Shimada N, Yoshida T, Ohno-Matsui K. Characteristics of peripapillarystaphylomas associated with high myopia determined by swept-source optical coherence tomography. Am.
J. Ophthalmol. 2016.06; 169; 138-144
11. Iwasaki Y, Sugita S, Mandai M, Yonemura S, Onishi A, Ito S, Mochizuki M, Ohno-Matsui K, Takahashi
M. Diﬀerentiation/puriﬁcation protocol for retinal pigment epithelium from mouse induced pluripotent
stem cells as a research tool. PLoS ONE. 2016.07; 11(7); e0158282
12. Yokoi T, Jonas JB, Shimada N, Nagaoka N, Moriyama M, Yoshida T, Ohno-Matsui K. Peripapillary
diﬀuse chorioretinal atrophy in children as a sign of eventual pathologic myopia in adults. Ophthalmology.
2016.08; 123(8); 1783-1787
13. Shinohara K, Moriyama M, Shimada N, Yoshida T, Ohno-Matsui K. Peripheral pigmented streaks in eyeswith pathologic myopia. Retina (Philadelphia, Pa.). 2016.08; 36(8); 1573-1578
14. Akaike S, Kamoi K, Tezsuka M, Tomizawa D, Yoshimura R, Takagi M, Ohno-Matsui K. Ocular manifestation in myeloid/NK cell precursor acute leukemia: a case report. Diagnosed by ﬂow cytometry and
PCR from aqueous humor. Medicine (Baltimore). 2016.09; 95(38); e4967
15. Karube H, Kamoi K, Ohno-Matsui K. Anti-TNF therapy in the management of ocular attacks in anelderly patient with long-standing Behçet’s disease. Int Med Case Rep J. 2016.09; 9; 301-304
16. Jonas JB, Bi HS, Wu JF, Xu L, Wang YX, Wei WB, Nangia V, Sinha A, Guo Y, You QS, Ohno-Matsui
K, Panda-Jonas S. Corneal curvature radius in myopia of schoolchildren versus adult myopia. Cornea.
2016.10; 35(10); 1333-1337
17. Hiroki Akiyama, Hiroshi Takase, Fumito Kubo, Tohru Miki, Masahide Yamamoto, Makoto Tomita, Manabu Mochizuki, Osamu Miura, Ayako Arai. High-dose methotrexate following intravitreal methotrexate
administration in preventing central nervous system involvement of primary intraocular lymphoma. Cancer Science. 2016.10; 107(10); 1458-1464
18. Ohno-Matsui K. Pathologic Myopia. Asia Pac J Ophthalmol (Phila). 2016.11; 5(6); 415-423
19. Yoshihiko Usui, Narsing A Rao, Hiroshi Takase, Kinya Tsubota, Kazuhiko Umazume, Daniel Diaz-Aguilar,
Takeshi Kezuka, Manabu Mochizuki, Hiroshi Goto, Sunao Sugita. Comprehensive polymerase chain
reaction assay for detection of pathogenic DNA in lymphoproliferative disorders of the ocular adnexa. Sci
Rep. 2016.11; 6; 36621[Books etc]
1. Koju Kamoi, Manabu Mochizuki. Intraocular Inﬂammation. Springer, 2016.01 (ISBN : 978-3-540-753872)[Misc]
1. Ohno-Matsui K, Lai TY, Lai CC, Cheung CM. Updates of pathologic myopia. Prog Retin Eye Res.
2016.05; 52; 156-187
2. Manabu Mochizuki, Koju Kamoi. F1000Prime Recommendation of [ Gül A et al., Ann Rheum Dis 2012,
71(4):563-6] . F1000 Prime.

― 226 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine[Conference Activities & Talks]

1. Kyoko Ohno-Matsui, Tomoka Ishida. OCT angiography for choroidal neovascularization due to pathologic
myopia. 39th Annual Macula Society Meeting 2016.02.23 Miami, USA
2. Kyoko Ohno-Matsui, Tomoka Ishida. Examinations of intrasclerally located Zinn-Haller ring by OCT
angiography. 39th Annual Macula Society Meeting 2016.02.24 Miami, USA
3. Ohno-Matsui K. Analyses of the Presence and Types of Posterior Staphyloma Using Wide-Field Imaging.
Fourth Annual International Retinal Imaging Symposium 2016.03.19 Los Angeles
4. Ohno-Matsui K. Pathologic ｍ yopia and its complications. 2016 APAO(Asia-Paciﬁc Academy of Ophthalmology) 2016.03.24 Taiwan,China
5. Ohno-Matsui K. Bruch membrane and myopic maculopathy. 2016 APAO(Asia-Paciﬁc Academy of Ophthalmology) 2016.03.24 Taiwan,China
6. Ohno-Matsui K. Bruch s membrane and pathologic myopia. 2016 APAO(Asia-Paciﬁc Academy of Ophthalmology) 2016.03.25 Taiwan,China
7. Ohno-Matsui K. Swept-source OCT in pathologic myopia. 2016 APAO(Asia-Paciﬁc Academy of Ophthalmology) 2016.03.25 Taiwan,China
8. Ohno-Matsui K. High myopia and myopia control forum. International Retinal Forum / Annual Meetingof Chinese Ocular Fundus Diseases Society 2016.04.14 China
9. Horie S, Ide M, Morohoshi K, Ohno-Matsui K. Evaluation of En-face OCT images in diabetic macularedema with anti-VEGF treatment. ARVO 2016 2016.05.02
10. Yoshida T, Ishida T, Kejia C, Shinohara K, Hongding L, Ohno-Matsui K. The correlation between sirt1
and muller cells in the development of CNV. ARVO 2016 2016.05.04 Seattle, USA
11. Shinohara K, Yoshida T, Ishida T, Honding L, Morita I, Ohno-Matsui K. Establishment of novel therapy
using ﬁbroblasts transplantation on sclera for experimental myopia in rats. ARVO 2016 2016.05.04
12. Nagaoka N, Morohoshi K, Jonas JB, Ohno-Matsui K. Optic disc size and visual ﬁeld defects in highmyopia. ARVO 2016 2016.05.05 Seattle, USA.
13. Ishii M, Ishida T, Morohoshi K, Ikegaya Y, Shinohara K, Ohono-Matsui K. Detection of Zinn-Haller
arterial ring in highly myopic eyes by optical coherence tomography angiography. ARVO 2016 2016.05.05
14. Ohno-Matsui K. Imaging of pathologic myopia. Samsung Medical Center Retina Symposium 2016.06.25
Seoul, Korea
15. Ohno-Matsui K, Alkabes M, Mateo C, Moriyama M. Features of posterior staphylomas analyzed by wideﬁeld fundus imaging and 3DMRI in patients with pathologic myopia. XXXth Meetinng of the Club Jules
Gonin 2016.07.07 Bordeaux, France
16. Ohno-Matsui K. Imaging of optic nerve in highly myopic eyes. XXII International society for Eye Research
Biennial Meeting(ISER） 2016.09.27 Tokyo
17. Ohno-Matsui K. Diagnosis of pathlogic myopia and over view. 3rd International conference on Pathologic
Myopia 2016.09.30 Tokyo
18. Ohno-Matsui K. Imaging of Pathologic Myopia. 3rd International conference on Pathologic Myopia
2016.10.01 Tokyo
19. Grzybowski A, Repka MX, Koﬄer BH, Ohno-Matsui K, Trier K, Morgan I, Tan D. New Approaches to
the Prevention and Treatment of Myopia. AAO(American Academy of Ophthalmology)2016 2016.10.17
Chicago,USA
20. Ohno-Matsui K, Cheung G, Lai T, Hirakata A. Diagnosis and Management of Complications due toPathologic Myopia. AAO(American Academy of Ophthalmology)2016 2016.10.17 Chicago,USA
21. Stanga P, Moussa MS, Ohno-Matsui K, Sadda SR. Spectral Domain and Swept Source OCT in the
Diagnosis and Management of Vitreoretinal and Uveitic Disorders. AAO(American Academy of Ophthalmology)2016 2016.10.18 Chicago,USA― 227 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

22. Ohno-Matsui K. Fundus imaging of Pathologic Myopia. The 70th Annual Congress of Japan Clinical
Ophthalmology 2016.11.03 Kyoto
23. Yokoi T, Ohno-Matsui K. Unilateral Multiple Drusenoid Lesions Detected by OCT in Eyes with High
Myopia. The 70th Annual Congress of Japan Clinical Ophthalmology 2016.11.04 Kyoto
24. Ohno-Matsui K. Peripapillary diﬀuse atrophy is an indicator in children for eventual pathologic myopia in
adults. 2016 Autumn Meeting of Ophthalmological Society of Taiwan 2016.11.13 Kaohsiung,Taiwan(Chung
Gung Memorial Hospital)
25. Ohno-Matsui K . Cutting edfe of ocular imaging for pathologic myopia. The 55th Annual Meeting ofJapanese Retina and Vitreous Society 2016.12.03 Tokyo[Others]
1. Pathologic Myopia-cutting edge of this blinding disease, 2016.03
Tung Wah Eastern Hospital― 228 ―

Cognitive
Cognitive andand Behavioral
Behavioral Medicine
Medicine

Otorhinolaryngology

Professor: Takeshi Tsutsumi
Associate Professor: Yoshiyuki Kawashima
Junior Associate Professor: Yasuhiro Suzuki
Assistant Professor: Taku Itou, Tarou Fujikawa, Yuichiro Inaba
Hospital Staﬀ: Takamori Takeda, Ayako Maruyama, Yumiko Tateishi,
Tomohide Hamajima, Tomoki O-oka
Graduate Student: Keiko Ohno, Ayane Makabe, Takamori Takeda, Motomu Honjo(1)

Research

1) Deafness gene analysis
2) Neurophysiological study of hearing
3) Histoanatomical study of ear, nose, throat, head, and neck4) Eye movement analysis in patients with dizziness5) Clinical study of treatment and prognosis in patients with allergic rhinitis, acute and chronic sinusitis, andbenign tumors6) Treatment of tinnitus
7) Treatment using endoscope8) Development of a new mapping procedure for cochlear implant
9) Bio-Marker of external ear canal carcinoma(2)

Lectures & Courses

Pre-graduate clinical education
Clinical systematic lecture covers anatomy, a general idea of diseases, their pathological conditions and treatments in the ﬁeld of otorhinolaryngology. Clinical clerkship I (general diagnostic training) provides instructionin the diagnosis and testing techniques of the otorhinolaryngological ﬁeld; clinical clerkship II (clinical training)provides detailed explanations of disease mechanisms, training in the performance of examinations, and clinicalresponsibilities involving both inpatient and outpatient care. Clinical clerkship III provides advanced training
beyond the scope of clinical clerkship II. In particular, students develop an advanced understanding of otorhinolaryngological diseases by conducting outpatient procedures (including taking histories, visual inspection, andpalpation), and gaining practical experience in assessment and diagnosis of patients’ conditions. Furthermore,in the clinical clerkshipIII, students also attend a micro-conference on teaching. Finally, students are assigned
to patients throughout their treatment, consistently dealing with the same individuals before, during, and after
surgery; this allows the students to become familiar with the course of clinical care.

(3)

Clinical Performances

Otorhinolaryngology clinic provides full examinations and treatment for diseases in ear, nose, throat, head,and neck, including dizziness, sudden deafness, facial palsy, infectious disease and benign as well as malignant
disease in the otorhinolaryngeal area. We have performed the ﬁrst implementation of bone anchored hearing aid
implant in Japan and since then we have experienced many patients for this surgery. We also have performed― 229 ―

Cognitive andand Behavioral
Behavioral Medicine
Medicine
Cognitive

surgery for patients with malignant disease as well as skull base lesions in collaboration with the Department of
the Head and Neck Surgery. Our outpatient clinic includes general ear, nose and throat clinic as well as allergy,
sinusitis, dizziness, otitis media, tumor, deafness, and tinnitus clinic.
(4)

Publications[Original Articles]
1. Yamamoto Y, Hirai C, Takeda T, Tasaki A, Inaba Y, Kiyokawa Y, Fujikawa T, Suzuki Y, Tsutsumi T..
The analysis of up and down-beating nystagmus on electronystagmography. Equilibrium Research. 2016;75; 106-114
2. Yoshihiro Noguchi, Masatoki Takahashi, Taku Ito, Taro Fujikawa, Yoshiyuki Kawashima, Ken Kitamura.
Delayed restoration of maximum speech discrimination scores in patients with idiopathic sudden sensorineural hearing loss. Auris Nasus Larynx. 2016.10; 43(5); 495-500
3. Ayako Maruyama, Atsunobu Tsunoda, Masatoki Takahashi, Seiji Kishimoto, Masami Suzuki. Nasopharyngeal pleomorphic adenoma presenting as otitis media with eﬀusion: case report and literature review.
Am J Otolaryngol. 35(1); 73-76
4. Masahiro Kishikawa, Atsunobu Tsunoda, Yoji Tanaka, Seiji Kishimoto. Large nasopharyngeal inverted
papilloma presenting with rustling tinnitus. Am J Otolaryngol. 35(3); 402-404[Conference Activities & Talks]
1. Takeda.T, Tasaki.A, InabaY, Kiyokawa.Y, Fujikawa.T, Suzuki.Y, Tsutsumi.T. Evaluating Listing s planefrom video-occulography on spinocerebellar degeneration. 29th Barany Society meeting 2016 2016.06
2. Tsutsumi T, Takeda T, Ikeda T, Fukuoka Y.. Evaluation of gravity perception using Listing s planeanalysis.. 29th Barany Society Meeting 2016.06.05― 230 ―

Cognitive
CognitiveandandBehavioral
BehavioralMedicine
Medicine

Neurology and Neurological Science
Professor YOKOTA Takanori
Junior Associate Professor ISHIBASHI Satoru
Junior Associate Professor
NISHIDA Yoichiro
Assistant Professor OHKUBO Takuya
Assistant Professor SATO Nozomu
Assistant Professor OZAKI Kokoro
Assistant Professor HATTORI Takaaki
Assistant Professor NUMASAWA Yoshiyuki
Project Professor SANJO Nobuo
Project Junior Associate Professor NAGATA Tetsuya
Project Assistant Professor KUWAHARA Hiroya
Project Assistant Professor YOSHIOKA Kotaro
Project Assistant Professor YUI Daishi
Project Assistant Professor SAKAUE Fumika
Project Researcher ASADA Ken
Graduate Student ITO Yoko
Graduate Student HIGASHI Miwa
Graduate Student Yagi Yohsuke
Graduate Student ZENIYA Satoshi
Graduate Student IWASAWA Eri
Graduate Student OHYAGI Masaki
Graduate Student HASEGAWA Jyuri
Graduate Student SHINTAKU Hiroshi
Graduate Student FURUKAWA Fumiko
Graduate Student MIYASHITA Akiko
Graduate Student FUJITA Kyohe
Graduate Student MAJIMA Takamasa
Graduate Student ONO Daisuke
Graduate Student HIRATA Kose
Graduate Student ISHIZU Nobutaka
Graduate Student LI Fu Ying
Graduate Student SONG Jin Dong
Graduate Student GUO Huijia
Graduate Student HU Yajun
Graduate Student ZHANG Yong Quan
Graduate Student SU SU Lei Mon
Graduate Student REYILA Mamuti
Graduate Student MAMUTI HASIYATI
Graduate Student DAIZO Kaiichi
Graduate Student YASUDA Eiji
Graduate Student MITSUHASHI Yuta
Graduate Student TAMURA Keigo
Graduate Student KUNIEDA Taiki
Research Student SANO Tatsuhiko
Research Student TAMAKI Toshihiro(1)

Research

1) Development of base technology on nucleic acid medicine and its application to neurological disorders
2) Discovery of biomarker in body ﬂuid for neurological diseases
3) Pathogenesis of Alzheimer disease4) Pathogenesis and therapies of amyotrophic lateral sclerosis (ALS)
5) Pathogenesis and therapies of cerebrovascular diseases― 231 ―

Cognitive andand Behavioral
Behavioral Medicine
Medicine
Cognitive6) Genetical and pathomechanical studies of spinocerebellar ataxias
7) Regulation of blood-brain barrier8) Electrophysiological studies9) Leading-edge neuroradiological studies10) Neuropathological studies of biopsied and autopsied samples(2)

Lectures & Courses

Neurology is a medical specialty concerned with the diagnosis and treatment of disorders of the nervous system
including the brain, spinal cord, peripheral nerves, autonomic nerves and skeletal muscles. Since the nervous
system extends to the whole body and regulate all the organs, neurologists have to examine and understandmany symptoms of the whole brain and body.
The Department of Neurology and Neurological Science at Tokyo Medical and Dental University oﬀers a uniqueclinical neurological training for specialist in a three-year residency program. This program is designed
to provide the highest quality clinical training in the clinical practice of neurology, either in an academic or apractice career. To accomplish this, the program integrates extensive practical exposure to all aspects of current
clinical neurology with a ﬁrm grounding in underlying scientiﬁc principles and methods of clinical investigations
such as electrophysiology, neuromuscular pathology, stroke, dementia, neuroimaging, and neurogenetics. The
faculty and staﬀ are committed to facilitate resident education and training.
After completion of their training for three years, senior residents are equipped with a lot of clinical experience
as attending doctors or teaching assistants in the university hospital and aﬃliated hospitals. They are eligible
for the board certiﬁcation by the Japanese Society of Neurology.

(3)

Clinical Services & Other Works

We daily see about 100 out-patients and 36 in-patients, and oﬀer in- and out-patient consultation services
through the weekday and on weekends. We diagnose and treat patients with stroke, multiple sclerosis, Parkinson’s disease, spinocerebellar ataxia, ALS, myopathies, neuropathies, meningitis/encephalitis, and hundreds ofother neurological issues. We also have the out-patients clinic specialized to patients with amnesia. Ourpatients will be reliably evaluated and diagnosed with some skillful techniques, such as the electrophysiological,
neuroradiological, and neuropsychological tests and pathological diagnosis of biopsied nerves and muscles.
(4)

Publications[Original Articles]
1. Tsutomu Nakamura, Fumiko Arima-Yoshida, Fumika Sakaue, Yukiko Nasu-Nishimura, Yasuko Takeda,
Ken Matsuura, Natacha Akshoomoﬀ, Sarah N Mattson, Paul D Grossfeld, Toshiya Manabe, Tetsu
Akiyama. PX-RICS-deﬁcient mice mimic autism spectrum disorder in Jacobsen syndrome through impaired GABAA receptor traﬃcking. Nat Commun. 2016; 7; 10861
2. Enomoto M, Hirai T, Kaburagi H, Yokota T. Eﬃcient Gene Suppression in Dorsal Root Ganglia andSpinal Cord Using Adeno-associated Virus Vectors Encoding Short-Hairpin RNA. Methods Mol Biol.
2016; 1364; 277-290
3. Yamada K, Kobayashi H, Bo R, Takahashi T, Purevsuren J, Hasegawa Y, Taketani T, Fukuda S, Ohkubo
T, Yokota T, Watanabe M, Tsunemi T, Mizusawa H, Takuma H, Shioya A, Ishii A, Tamaoka A, Shigematsu Y, Sugie H, Yamaguchi S. Clinical, biochemical and molecular investigation of adult-onset glutaricacidemia type II: Characteristics in comparison with pediatric cases. Brain Dev. 2016; 38(3); 293-301
4. Nobuo Sanjo,Satoko Kina,Yukiko Shishido-Hara,Yurie Nose,Satoru Ishibashi,Tetsuya Fukuda,Taketoshi
Maehatra,Yoshinobu Eishi,Hidehiro Mizusawa,Takanori Yokota. A Case of Progressive Multifocal Leukoencephalopathy with Balanced CD4/CD8 T-Cell Inﬁltration and Good Response to Meﬂoquine Treatment.
Internal Medicine. 2016.01; 55(12); 1631-1635
5. Eric Vallabh Minikel,Sonia M. Vallabh,Monkol Lek,Karol O. Estrada,Kaitline E. Samocya,J. Fah Sathirapongsasuti,Cory Y. McLean,Joice Y. Tung,Linda P.C.Yu, Pierluigi Gambetti,Janis Blevins,Shuling― 232 ―

Cognitive
CognitiveandandBehavioral
BehavioralMedicine
Medicine

Zhamg,Yvonne Cohen,Wei Chen,Masahito Yamada,Tsuyoshi Hamaguchi,Nobuo Sanjo,Hidehiro Mizusawa,Yoshikazu
Nakamura,Tetsuyuki Kitamoto,Steven J. Collins,Alison Boyd,Robert G Will,Richard Knight,Claudia Ponto,Inga
Zerr,Theo Kraus,Sabina Eigenbrod,Armin Giese,Jesus de Pedro Cuesta,Stéphane Haïk,Jean-Louis Laplanche,Jean Philippe Brandel,Michael Boenke,Markku Laakso,Karen Mohlke,Francis S.Collins,Anna Kähler,Kinberly Chambert, Steven McCarroll,Patrick Sullivan,Christina M. Hultman,Shaun M.Purcell,Pamela
Sklar,Cornelia M.van Dujin,F.Rivadeneira Ramirez,Arfan Ikram,Sven J.van der Lee,Jeannette M.VergeerDrop,André G.Uitterlinden,Mark J.Daley,Daniel G.MacArthur,. Quantifying prion disease penetrance
using large population control cohorts. Science Translational Medicine 2016. 2016.01; 8(322); 322R9322RA9
6. Yokota T, Kanouchi T, Ohkubo T. ALS: Quantitative analysis of disease progression from a new point ofview 2015 Annual report of the research committee of CNS degenerative diseases, The ministry of health,labour and welfare of Japan. 2016.03; 58-61
7. Mutsuki Kuraoka, En Kimura, Tetsuya Nagata, Takashi Okada, Yoshitsugu Aoki, Hisateru Tachimori,
Naohiro Yonemoto, Michihiro Imamura, Shin’ichi Takeda. Serum Osteopontin as a Novel Biomarker for
Muscle Regeneration in Duchenne Muscular Dystrophy. Am. J. Pathol.. 2016.05; 186(5); 1302-1312
8. Koichi Kimura, Hiroyuki Morita, Masao Daimon, Masaru Horio, Takayuki Kawata, Tomoko Nakao,
Megumi Hirokawa, Ruriko Kitao, Daisuke Watanabe, Tetsuo Komori, Tetsuya Nagata, Shin’ichi Takeda,
Hirofumi Komaki, Kazuhiko Segawa, Takashi Nakajima, Katsu Takenaka, Issei Komuro. Utility of Cystatin C for Estimating Glomerular Filtration Rate in Patients With Muscular Dystrophy. Int Heart J.
2016.05; 57(3); 386-388
9. Koya Ono, Ken Murata, Toshinari Arai, Tomoyuki Kino, Keisuke Onaka, Satoru Takahashi, Hirotaka
Sato, Hiroya Kuwahara. Posterior reversible encephalopathy syndrome after iron administration in apatient with chronic severe anemia. Journal of the Neurological Sciences. 2016.08; 367; 333-334
10. Mitsuru Yoneyama,Hiroshi Mitoma,Nobuo Sanjo, Maya Higuma,Hiroo Terashi,Takanori Yokota. Ambulatory Gait Behavior in Patients with Dementia: A Comparison with Parkinson’s Disease. Transactions
on Neural System and Rehabilitation Engineering 2015 . 2016.08; 24(8); 817-826
11. Ryo Yamasaki, Takuya Matsushita, Toshiyuki Fukazawa, Kazumasa Yokoyama, Kazuo Fujihara, Mieko
Ogino, Takanori Yokota, Katsuichi Miyamoto, Masaaki Niino, Kyoichi Nomura, Ryo Tomioka, Masami
Tanaka, Izumi Kawachi, Takashi Ohashi, Ken-Ichi Kaida, Makoto Matsui, Yuji Nakatsuji, Hirofumi Ochi,
Hikoaki Fukaura, Takashi Kanda, Akiko Nagaishi, Kanae Togo, Hidehiro Mizusawa, Hiroyuki Murai, JunIchi Kira. Eﬃcacy of intravenous methylprednisolone pulse therapy in patients with multiple sclerosis andneuromyelitis optica. Mult. Scler.. 2016.09; 22(10); 1337-1348
12. Hitoshi Suzuki, Yoshitsugu Aoki, Toshiki Kameyama, Takashi Saito, Satoru Masuda, Jun Tanihata, Tetsuya Nagata, Akila Mayeda, Shin’ichi Takeda, Toshifumi Tsukahara. Endogenous Multiple Exon Skipping
and Back-Splicing at the DMD Mutation Hotspot. Int J Mol Sci. 2016.10; 17(10);
13. Nobutaka Ishizu, Daishi Yui, Akira Hebisawa, Hidenori Aizawa, Wanpeng Cui, Yuko Fujita, Kenji
Hashimoto, Itsuki Ajioka, Hidehiro Mizusawa, Takanori Yokota, Kei Watase. Impaired striatal dopamine
release in homozygous Vps35 D620N knock-in mice. Hum. Mol. Genet.. 2016.10; 25(20); 4507-4517
14. Nobuko Yamaguchi, Sonoko Misawa, Yasunori Sato, Kengo Nagashima, Kanako Katayama, Yukari Sekiguchi,
Yuta Iwai, Hiroshi Amino, Tomoki Suichi, Takanori Yokota, Yoichiro Nishida, Nobuo Kohara, Koichi Hirata, Kazutoshi Nishiyama, Ichiro Yabe, Ken-Ichi Kaida, Norihiro Suzuki, Hiroyuki Nodera, Shoji Tsuji,
Haruki Koike, Jun-Ichi Kira, Hideki Hanaoka, Susumu Kusunoki, Satoshi Kuwabara, . A Prospective,
Multicenter, Randomized Phase II Study to Evaluate the Eﬃcacy and Safety of Eculizumab in Patientswith Guillain-Barré Syndrome (GBS): Protocol of Japanese Eculizumab Trial for GBS (JET-GBS). JMIRRes Protoc. 2016.11; 5(4); e210
[Misc]
1. Yutaro Asami, Kotaro Yoshioka, Kazutaka Nishina, Tetsuya Nagata, Takanori Yokota. Drug delivery
system of therapeutic oligonucleotides. Drug Discov Ther. 2016; 10(5); 256-262
2. Eri Iwasawa, Masahiko Ichijo, Satoru Ishibashi, Takanori Yokota. Acute development of collateral circulation and therapeutic prospects in ischemic stroke. Neural Regen Res. 2016.03; 11(3); 368-371― 233 ―

CognitiveandandBehavioral
BehavioralMedicine
Medicine
Cognitive

3. Masaki Ohyagi, Takanori Yokota. Oligonucleotide therapeutics for treatment of neurological degenerative
diseases 2016.08; 67(4); 349-353
4. Takanori Yokota, Kazutaka Nishina, Hiroya Kuwahara. Gene Therapy with therapeutic oligonucleotides
Neurological Therapeutics. 2016.11; 33(3); 303-306[Conference Activities & Talks]
1. Amano E, Suzuki M, Ozaki K, Ishibashi S, Akaza M, Kanouchi T, Tohda S, Yokota T. A case of sensory
dominant CIDP with conduction delay predominantly in the most distal segment (Japanese). 10th Tokyo
Metropolitan Neuromuscular Electrodiagnosis Forum 2016.01.30 Tokyo
2. Asada K, Canestrari E, Fu X, Li Z, Makowski E, Wu YC, Mito JK, Kirsch DG, Baraban J, Paroo Z..
Rescuing dicer Defects via Inhibition of an Anti-dicing Nuclease. The 2nd Annual Meeting of the Japan
miRNA Society 2016.02.06 Nagoya
3. Song J, Shimoura T, Kuwahara H, Yoshida-Tanaka K, Nishina K,, Nagata
T,, Yokota T. New platformgtechnology to regulate the blood-brain barrier function in vivo. 第 8 回 CBIR+ONSA 共催若手インスパイ
アシンポジウム 2016.02.20 Tokyo
4. Takanori Yokota. DNA/RNA heteroduplex oligonucleotide as a novel concept of therapeutic oligonucleotide. 8th AsiaTIDES Oligonucleotide and Peptide Therapeutics 2016.02.25 Kyoto
5. Takanori Yokota. DNA/RNA heteroduplex oligonucleotide for highly eﬃcient gene silencing. Oligonucleotide Therapeutics & Delivery 2016.04.04 Boston
6. Hiroya Kuwahara, Takahiro Shimoura, Jindong Song, Kie Yoshida-Tanaka, Kazutaka Nishina, Tetsuya
Nagata, Takanori Yokota. Hetero-duplex oligonucleotide to regulate the blood-brain barrier function in
vivo. Oligonucleotide Therapeutics & Delivery 2016.04.04 Boston
7. Fumiko Furukawa ,Nobuo Sanjo,Atsushi Kobayashi,Tsuyoshi Hamaguchi,Masahito Yamada,Tetsuyuki Kitamoto,Hidehiro Mizusawa. Diﬀerential association of amyloid-β with PrPSc pathology in each genetic
prion disease.. Prion2016 2016.05.10 Tokyo,JPN
8. Ryusuke Ae,Yoshikazu Nakamura, Ichiro Takumi,Nobuo Sanjo,Tetsuyuki Kitamoto,Hidehiro Mizusawa.
Epidemiologic features of human prion diseases in Japan: a prospective 15-year surveillance study..
Prion2016 2016.05.10 Tokyo,JPN
9. Kenji Sakai,Tsuyoshi Hamaguchi,Nobuo Sanjo,Hiroyuki Murai,Moeko Noguchi-Shinohara,Ichiro Nozaki,Yoshikazu
Nakamura,Tetsuyuki Kitamoto,Hidehiro Mizusawa,Masahito Yamada. Diﬀusion-weighted images in patients with dura mater garft-assosiated Creutzfeldt-Jakob disease.. Prion2016 2016.05.10 Tokyo,JPN
10. Yuko Ishimura,Tadashi Tsukamoto,Kazuo Kuwata,Masahito Yamada,Katsumi Doh-ura,Yoshio Tsuboi,Katsuya
Sato,Yoshikazu Nakamura,Nobuo Sanjo,Chieko Tamura,Hidehiro Mizusawa . The Japan Consortiumof Prion diseases (JACOP) for patients’ registration and clinical studies of Prion diseases in Japan. .
Prion2016 2016.05.10 Tokyo,JPN
11. Ichiro Takumi,Nobuhito Saito,Nobuo Sanjo,Shunsaku Takayanagi,Chieko Tamura,Tadashi Tsukamoto,Yoshiyuki
Kuroiwa,Ryusuke Ae,Yoshikazu Nakamura,Tetsuyuki Kitamoto,Tsuyoshi Hamaguchi,Masahito Yamada,Hidehiro
Mizusawa. CJD incidents in Japan.. Prion2016 2016.05.10 Tokyo,JPN
12. Tadashi Tsukamoto,Ryusuke Ae,Yoshikazu Nakamura,Nobuo Sanjo,Tetsuyuki Kitamoto,Katsuya Satoh,Tsuyoshi
Hamaguchi,Masahito Yamada,Hidehiro Mizusawa. Human Prion Diseases Surveillance and registration
system in Japan.. Prion2016 2016.05.10 Tokyo,JPN
13. Tsuyoshi Hamagchi,Kenji Sakai,Tetsuyuki Kitamoto,Masaki Takao,Shigeo Murayama,Yasushi Iwasaki,Mari
Yoshida,Hiroshi Shimizu,Akiyoshi Kakita,Hitoshi Takahashi,Hiroyoshi Suzuki,Hironobu Naiki,Nobuo Sanjo,Hidehiro
Mizusawa,Masahito Yamada. Cerebral β-Amyloidsis in patients with dura mater graft-associated CreutzfeldtJakob disease.. Prion2016 2016.05.10 Tokyo,JPN
14. Kanouchi T, Sekiguchi T, Yokota T. A pilot study on disease progression of ALS by quantitative analysis
from a new viewpoint. 57th annual meeting of the Japanese Society of Neurology 2016.05.18 Kobe

― 234 ―

Cognitive
CognitiveandandBehavioral
BehavioralMedicine
Medicine

15. Jindong Song, Tetsuya Nagata, Wenying Piao, Kazutaka Nishina, Takuya Okubo, Keisuke Abe, Masato
Hasegawa, Takanori Yokota. Inhibition of alpha-synuclein ﬁbril assembly by antisense oligonucleotide inmice. 57th Annual Meeting of the Japanese Society of Neurology 2016.05.18 Kobe
16. Fuying Li, Satoru Ishibashi, Kotaro Yoshioka, Takehiko Wada, Masahiko Ichijo, Eri Iwasawa, Jindong
Song, Yongquan Zhang, Takanori Yokota. Heteroduplex oligonucleotide reduced gene expression in focal
ischemic brain in mice. 57th Annual Meeting of the Japanese Society of Neurology 2016.05.18 Kobe
17. Nobuo Sanjo, Hidehiro Mizusawa, Takanori Yokota. Various neurological manifestations of lymphoma.
57th Annual Meeting of the Japanese Society of Neurology 2016.05.18 Kobe
18. Yoichiro Nishida, Winkler Ethan, Sagare Abhay, De Vivo Darryl, Zlokovic Berislav. GLUT1 reductions
at the BBB exacerbate Alzheimer disease vasculo-neuronal dysfunction. 57th Annual Meeting of theJapanese Society of Neurology 2016.05.19 Kobe
19. Ogata H, Yamasaki R, Oka N, Kuwahara M, Suzuki H, Kusunoki S, Yagi Y, Yokota T, Matsushita T,
Kira J-I. Useful laboratory markers for predicting anti-NF155 antibody status among CIDP patients. 57th
Annual Meeting of the Japanese Society of Neurology 2016.05.19 Kobe
20. Hiroya Kuwahara, Takahiro Shimoura, Jindong Song, Kie Yoshida-Tanaka, Kazutaka Nishina, Tetsuya
Nagata, Takanori Yokota. A novel platform technology to regulate the blood-brain barrier in vivo. 57th
Annual Meeting of the Japanese Society of Neurology 2016.05.19 Kobe
21. Kazutaka Nishina, Kotaro Yoshioka, Tomoko Nishina, Hiroya Kuwahara, Tetsuya Nagata, Takanori
Yokota. Novel oligonucleotide based on DNA/RNA heteroduplex structures. 57th Annual Meeting of
the Japanese Society of Neurology 2016.05.19 Kobe
22. Nozomu Sato, Kinya Ishikawa, Hidehiro Mizusawa, Takanori Yokota. Variations in the mutation forspinocerebellar ataxia type 31 require cautious genetic testing. 57th Annual Meeting of the JapaneseSociety of Neurology 2016.05.19 Kobe
23. Eri Iwasawa, Satoru Ishibashi, Masahiko Ichijo, Fu Ying Li, Takanori Yokota. Upregulation and distribution of sphingosine-1-phosphate receptor 1 in acute ischemic stroke. 57th Annual Meeting of the JapaneseSociety of Neurology 2016.05.19 Kobe
24. Kotaro Yoshioka, Taiki Kunieda, Kie Tanaka, Wenying Piao, Hiroya Kuwahara, Kazutaka Nishina, Tetsuya Nagata, Takanori Yokota. A new type of double–stranded structure improved potency of antisense
oligonucleotide for FAP. 57th Annual Meeting of the Japanese Society of Neurology 2016.05.19 Kobe
25. Keisuke Abe, Mio Tajiri, Daishi Yui, Hiroki Sasaguri, Takuya Ohkubo, Takanori Yokota. A C-terminal
fragment of TDP-43 in exosomes mediates propagation of ALS pathology. 57th Annual Meeting of theJapanese Society of Neurology 2016.05.20 Kobe
26. Kazumasa Soga, Kinya Ishikawa, Tokuro Huruya, Hidehiro Mizusawa, Takanori Yokota. A clinical andneuropathological study on homozygous mutations with spinocerebellar ataxia type 6. 57th Annual Meeting of the Japanese Society of Neurology 2016.05.20 Kobe
27. Toshiro Kanazawa, Yurie Nose, Yutaro Asami, Jin Nonami, Masahide Iijima, Hiroaki Tanaka, Takanori
Yokota. Clinical features of patients with motor ﬂuctuations before intravenous thrombolysis. 57th Annual
Meeting of the Japanese Society of Neurology 2016.05.20 Kobe
28. Masahiko Ichijo, Satoru Ishibashi, Kazunori Miki, Eri Iwasawa, Kyohei Fujita, Hiroaki Yokote, Takeshi
Amino, Tomoyuki Kamata, Takanori Yokota. Signiﬁcance of development and reversion of collaterals
in outcome after intravenous thrombolysis. 57th Annual Meeting of the Japanese Society of Neurology
2016.05.20 Kobe
29. Shintaku H, Kanouchi T, Yokota T. Pathophysiology of myoclonus in corticobasal syndrome. 57th annual
meeting of the Japanese Society of Neurology 2016.05.21 Kobe
30. Hiroshi Doi, Shigeru Koyano, Misako Kunii, Shunta Hashiguchi, Hitaru Kishida, Kenichi Tanaka, Masaaki
Shiina, Kazuhiro Ogata, Fumiko Hirashima, Yukichi Inoue, Nozomu Sato, Kokoro Ozaki, Kiyobumi Ohta,
Takanori Yokota, Hidehiro Mizusawa, Jun Mitsui, Shoji Tsuji, Naomichi Matsumoto, Kinya Ishikawa,
Fumiaki Tanaka. The clinical and pathological features of autosomal-dominant SCA with CACNA1G
mutation. 57th Annual Meeting of the Japanese Society of Neurology 2016.05.21 Kobe

― 235 ―

Cognitive andand Behavioral
Behavioral Medicine
Medicine
Cognitive

31. Hiroya Kuwahara, Hiroto Fujigasaki, Tohru Tanizawa, Ken Matsumura, Mutsufusa Watanabe, Takanori
Yokota, Toshiki Uchihara. An autopsy case of ALS with argyrophilic grain disease showing PSP-likecytopathology predominantly in cerebral cortex. The 57th Annual Meeting of the Japanese Society of
Neuropathology 2016.06.02 Hirosaki
32. Ogata H, Yamasaki R, Oka N, Kuwahara M, Suzuki H, Kusunoki S, Yagi Y, Matsushita T, Yokota T, Kira
J-I. Valuable objective markers for predicting anti-neurofascin 155 antibody status among CIDP patients.
The International Inﬂammatory Neuropathy Consortium 2016 2016.06.22 Glasgow
33. Taiki Kunieda, Kotaro Yoshioka, Yumiko Sujino, Kie Tanaka, Wenying Piao, Hiroya Kuwahara, Kazutaka
Nishina, Tetsuya Nagata, Takanori Yokota. Development of antisense oligonucleotide with a new type ofa double stranded structure. The RNA society of Japan 18th annual meeting (The 21st annual meetingof the RNA society) 2016.06.29 Kyoto
34. Kotaro Yoshioka, Yumiko Sujino, Taiki Kunieda, Kie Tanaka, Wenying Piao, Hiroya Kuwahara, Kazutaka
Nishina, Tetsuya Nagata, Takanori Yokota. A new mechanism of microRNA inhibition by a new type of
duplex oligonucleotide. The RNA society of Japan 18th annual meeting (The 21st annual meeting of the
RNA society) 2016.06.29 Kyoto
35. Fumika Sakaue, Eiji Yasuda, Tatsuhiko Sano, Taro Ishiguro, Tetsuya Nagata, Takanori Yokota. Chemically modiﬁed RNAs inhibit TDP-43 aggregation. The RNA society of Japan 18th annual meeting (The
21st annual meeting of the RNA society) 2016.06.29 Kyoto
36. Kazutaka Nishina, Kie Yoshida-Tanaka, Wenying Piao, Takanori Yokota. DNA/RNA heteroduplex
oligonucleotide (HDO) for highly eﬃcient gene silencing. The RNA society of Japan 18th annual meeting(The 21st annual meeting of the RNA society) 2016.06.30 Kyoto
37. Yoko Ito,Nobuo Sanjo,Yukiko Matsumoto,Takanori Yokota. Optimal starting time of cholinesterase inhibitors for amnestic MCI.. Alzheimer’s Association International Conference 2016 2016.07.14 Toronto,Canada
38. Fumiko Furukawa, Nobuo Sanjo, Atsushi Kobayashi, Tsuyoshi Hamaguchi, Masahito Yamada, Tetsuyuki
Kitamoto, Hidehiro Mizusawa, Takanori Yokota. Colocalization of A β 42 with PrPSc-plaques in thebrain of the Gerstmann-Sträussler-Scheinker disease with the P105L mutation. Alzheimer’s Association
International Conference 2016 2016.07.24 Toronto
39. Yoichiro Nishida, Daishi Yui, Tomoko Nishina, Kaoru Mogushi, Satoru Ishibashi, Itsuki Ajioka, Kinya
Ishikawa, Hidehiro Mizusawa, Shigeo Murayama, Takanori Yokota. Enhanced Pla2g3 expression by oxidative stress decreases the IDE and exacerbates AD. Alzheimer’s Association International Conference
2016 2016.07.26 Toronto
40. Takanori Yokota. Propagation of TDP-43 in ALS. Propagation in Neurodegenerative Disease Conference
2016 2016.08.11 Dublin
41. Masahito Yamada,Tsuyoshi Hamaguchi,Yu Taniguchi,Kenji Sakai,Tetsuyuki Kitamoto,Masaki Takao,Shigeo
Murayama,Yasushi Iwasaki,Mari Yoshida,Hiroshi Shimizu,Akiyoshi Kakita,Hitoshi Takahashi,Hiroyoshi
Suzuki,Hironobu Naiki,Nobuo Sanjo,Hidehiro Mizusawa,. Possible iatrogenic transmissison of cerebral
amyloid angiopathy and subpialA β deposition via cadaveric dura mater grafting.. 5th International
CAA conference 2016 2016.09.08 Boston,USA
42. Hiroya Kuwahara. Development of nucleic acid therapeutics to target the blood-brain barrier. The 22nd
Symposium of Forum for Pharmaceutical Technology Innovation 2016.09.09 Tokyo
43. Hiroya Kuwahara, Takahiro Shimoura, Jindong Song, Kie Yoshida-Tanaka, Tadahaya Mizuno, Tatsuki
Mochizuki, Masaki Ohyagi, Kazutaka Nishina, Tetsuya Nagata, Hiroyuki Kusuhara, Takanori Yokota.
New platform technology to regulate the blood-brain barrier function in vivo. 19th International Symposium on Signal Transduction at the Blood-Brain Barriers 2016.09.15 Copenhagen
44. Taiki Kunieda, Kotaro Yoshioka, Yumiko Sujino, Kie Tanaka, Wenying Piao, Hiroya Kuwahara, Kazutaka
Nishina, Tetsuya Nagata, Takanori Yokota. Pharmacokinetic study of double stranded antisense oligonucleotide: Hetero Chimera Duplex Oligonucleotide (HCDO). 12th Annual Meeting of the Oligonucleotide
Therapeutics Society 2016.09.26 Montreal― 236 ―

Cognitive
CognitiveandandBehavioral
BehavioralMedicine
Medicine

45. Kotaro Yoshioka, Yumiko Sujino, Taiki Kunieda, Kie Tanaka, Wenying Piao, Hiroya Kuwahara, Kazutaka
Nishina, Tetsuya Nagata, Takanori Yokota. Intracellular mechanisms of microRNA inhibition by a new
double-stranded structure for therapeutic oligonucleotide. 12th Annual Meeting of the Oligonucleotide
Therapeutics Society 2016.09.27 Montreal
46. Takanori Yokota. DNA/RNA heteroduplex oligonucleotide. 12th Annual Meeting of the Oligonucleotide
Therapeutics Society 2016.09.27 Montreal
47. Yohsuke Yagi, Takuya Ohkubo, Hideya Kawaji, Akira Machida, Haruka Miyata, Saori Goda, Sugata
Roy, Yoshihide Hayashizaki, Harukazu Suzuki, Takanori Yokota. Small RNA proﬁling of cerebrospinal
ﬂuid exosomes using next-generation sequencing. American Society for Exosomes and Microvesicles 20162016.10.21 California
48. Iida S, Kanouchi T, Suzuki M, Sanjo N, Nishida Y, Yokota T. Distribution patterns of nerve conduction
abnormality in CIDP. 46th annual meeting of the Japanese Society of Clinical Neurophysiology 2016.10.28
Kohriyama
49. Hiroya Kuwahara, Takahiro Shimoura, Jindong Song, Kie Yoshida-Tanaka, Tadahaya Mizuno, Tatsuki
Mochizuki, Kazutaka Nishina, Tetsuya Nagata, Hiroyuki Kusuhara, Takanori Yokota. Novel oligonucleotide to regulate the blood-brain barrier. 3rd International Conference on Biomaterials Science 2016.11.28
Tokyo
50. Takanori Yokota. DNA/RNA heteroduplex oligonucleotide for highly eﬃcient gene silencing. 3rd International Conference on Biomaterials Science. 2016.11.30 Tokyo
51. Noriko Nakamura, Yasutaka Anraku, Hiroya Kuwahara, Takanori Yokota, Kazunori Kataoka. Development of innovative therapeutic nanomachines for intractable neurological diseases. 3rd COINS International Symposium 2016.12.15 Kawasaki
52. Hiroya Kuwahara, Yasutaka Anraku, Kazunori Kataoka, Takanori Yokota. Investigation of delivery mechanism of BBB-crossing nanomachine. 3rd COINS International Symposium 2016.12.15 Kawasaki― 237 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

Psychiatry and Behavioral Sciences

Professor
Toru NISHIKAWA,Takayuki OKADA
Associate Professor
Akeo KURUMAJI
Junior Associate Professor
Naoki YAMAMOTO(∼2016.6),Takashi TAKEUCHI
Assistant Professor
Daisuke JITOKU,Akihito UEZATO,Hiroo MITSUSADA(∼2016.3),
Yuichiro ABE,Kohei HINO(∼2016.3),Hitoshi MUTO(2016.4∼),
Mizue HOBO(Department of Sleep Modulatory Medicine)(∼2016.3),
Takehiro TAMURA(2016.7∼),Shunsuke TAKAGI(2016.4∼9),
Takako NAKANOTANI(2016.10∼)
Medical Staﬀ
Hitoshi MUTO(∼2016.3),Takehiro TAMURA(∼2016.6),
Shunsuke TAKAGI(∼2016.3),Ryotaro SAITO(∼2016.3),
Hiroki SHIWAKU,Kohei MISE(2016.4∼),Satoshi TAKAHASHI(2016.4∼),
Tomonori TOMISHIGE(2016.4∼),Masashi NAGASE(2016.7∼),
Mariko SUNAHARA(2016.12∼)
Medical Fellow
Sayuri ISHIWATA(∼2016.3)
Technical AssistantAsami UMINO, Sayuri ISHIWATA(2016.4∼12),Masakazu UMINO,
Momoko KOBAYASHI(∼2016.3)
Clinical Psychologist
Kazunori MURAKAMI,Yukari WAKAYAMA,Yasuhiro OKA,
Miyuki SAITO,Hisashi YAMADA
Psychiatric Social Worker
Yoshifumi KANEKO,Noriko NUMAGUCHI,
Ayano SOMEYA(∼2016.3),Sayaka KOJIMA
Graduate Students
Masakazu UMINO,Kazuo TAKIGUCHI, Momoko KOBAYASHI,
Megumi GOTO,Ko FURUTA,Shigehiro OGATA,Hidetoshi KINOSHITA,
Ryotaro SAITO,Koji TAKEDA, Mai KATO,Kanae HAYAKAWA(1)

OutlineOur laboratory is committed to comprehensive research on endogenous psychosis， neurosis， and epilepsy
through biological, psychological and social approaches. In collaboration with external research facilities, weare also involved in social psychiatry. child and adolescent psychiatry, and brain imaging studies.
(2)

Research

1)Studies in neurochemistry(i)Molecular genetic studies to clarify the causes and conditions of neuropsychiatric diseases:― 238 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

Using animal models with psychotic symptom-causing agents, we are involved in a study to isolate new candidate gene clusters associated with the pathogenesis and pathophysiology of neuropsychiatric disorders from theviewpoint of developmental pharmacology. We are examining the eﬀects of candidate gene clusters in patientswith neuropsychiatric disorders.
(ii)Studies in biochemical pharmacology to develop new therapeutic methods for neuropsychiatric disorders.
2) Neurophysiological and psychophysiological studies(i)Studies of neurotransmitter receptor binding in neuropsychiatric disorders with PET:
We are working together with the National Institute of Radiological Sciences to investigate the binding activitiesof dopamine receptors in various brain areas of the patients with schizophrenia and mood disorders.
(ii)A study of sleep stages and behavior in neuropsychiatric diseases:
A study is being carried out to examine sleep stages and behavior using an originally developed automatic
analysis device (polysomnography) in patients with various psychiatric disorders.
A study on brain functioning in psychiatric disorders by using the near-infrared spectroscopy (NIRS): To obtainan insight into biological markers of psychiatric disorders, changes in regional brain functions during psychological tasks are examined by measuring the relative concentrations of oxyhemoglobin using NIRS in combination
with MRI in the brain areas of the patients with schizophrenia and mood disorders.
3) Psychopathological studies
We are conducting psychological studies of neuropsychiatric diseases from the aspects of phenomenology, anthropology, and linguistics, while employing a psychotherapeutic approach. Other research activities include a
review of basic psychiatric concepts and a basic study for the c1assiﬁcation and diagnosis of psychiatric disorders, which are important recent issues. In addition to endogenous psychosis including schizophrenia and manic
depressive disorder. we are also involved in psychoanalytic studies of neurosis and borderline personality disorder, which are attracting increasing attention, and psychotherapies for them, as well as pathological researchon pathography and art therapy in terms of creativity.
(3)

Education

Following the two-year period of mandatory clinical training, basic professional training in psychiatry will be
provided for 6-9 months mainly in the university. In the second term of training, they will acquire knowledge andclinical experience necessary for neuropsychiatrists，and undergo practical training at aﬃliated medical facilities
to become qualiﬁed psychiatrists. Undergraduate education, which places emphasis on clinical clerkship training
after a systematic series of lecture course and seminar-based classes, is designed to develop students’ problemsolving skills, and increase their motivation to learn neuropsychiatry, with support from external facilities.
(4)

Lectures & Courses

In the ﬁrst term (two years) of postgraduate training, residents will learn basic laboratory procedures anddiagnostictechniques,psychotherapy and drug treatment and laws and regulations related to clinical practice,
and acquire other general knowledge，all being essential for biologic ，la psychological, social, and ethicalapproaches to neuropsychiatric diseases.
(5)

Clinical Services & Other Works

Clinical practiceApproximately eighty new outpatients visit our department every month, about 30% of which are classiﬁed ashaving mood disorders" (F3) by ICO-10, followed by "neurotic, stress-related, and somatoform disorders" (F4)and schizophrenia, schizophrenic and paranoid disorders" (F2). We are also actively involved in consultationand liaison psychiatry for inpatients in other departments. Patients with snile dementia, child and adolescent
psychiatric disorders, substance, dependence, and neurosis requiring intensive psychotherapy are often referred
to related and advanced facilities for specialized treatment. Since this facility, the psychiatric department of ageneral hospital, is used for university education and training, most inpatients are c1assiﬁed as F2, followed by F4
and F3 (ICD-10). We also provide care and treatment for patients with sleep rhythm disorders and neurologicaldisorders, including epilepsy and senile dementia. In addition to drug treatment, we have introduced andprovided mECT (modiﬁed electroconvulsive therapy) for inpatients, and individual and group psychotherapy
for the patients in our psychiatric ward and clinic and day care center in close collaboration with rehabilitation
facilities in the community. The day care team consists of a doctor, two nurses. And a psycho-social-worker
or a clinical psychologist. Day care (partial hospitalization) is the transitional element between inpatient― 239 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

and outpatient care and its indications have a wide range of psychiatric disorders as follows: schizophrenia,
depression, bipolar disorder, adjustment disorder and personality disorders. Each member has the own aim andthe team gives care with diﬀerent types of framework. Our day care team regards the potentiality of group very
important and the group process could contribute to therapeutic eﬀect. With this kind of experience, patients
could develop their ability to communicate with other people and readapt to social situations.
(i)A study of biological indicators in schizophrenia with eye cameras:
We are not only involved in studies of monozygotic twins，early-onset patients，and children at a high risk inJapan, but also in an international joint research project of the WHO as a center in charge of operations.
(6)

Clinical Performances

Since the 2011 ﬁscal year, we have been conducting the clinical trial of D-cycloserine for negative symptoms
such as avolition, ﬂattening of aﬀect, and poverty of thought and cognitive dysfunction of schizophrenia. We
also have been treating patients with schizophrenia with clozapine, which has been approved for refractoryschizophrenia. We have started getting systematically involved in the treatment of patients complaining of painor dysesthesia in oral regions in liaison with the Dental Hospital, which is the unique characteristics of ouruniversity. In the 2014 ﬁscal year, we started the psychoeducational program for patients with bipolar disorder,
which focuses on the prevention of relapse. In addition, there is an increasing number of liaison activities forthe prevention of delirium especially in the surgical wards, the intervention to patients with suicide attempts inthe emergency room, and the mental support for peripartum patients in cooperation with the staﬀs.
(7)

Publications[Original Articles]
1. Mizue Hobo, Akihito Uezato, Mitsunori Nishiyama, Mayumi Suzuki, Jiro Kurata, Koshi Makita, Naoki
Yamamoto, Toru Nishikawa. A case of malignant catatonia with idiopathic pulmonary arterial hypertension treated by electroconvulsive therapy. BMC Psychiatry. 2016; 16(1); 130
2. Yojiro Umezaki, Anna Miura, Motoko Watanabe, Miho Takenoshita, Akihito Uezato, Akira Toriihara,
Toru Nishikawa, Akira Toyofuku. Oral cenesthopathy. Biopsychosoc Med. 2016; 10; 20
3. Dean B, Gibbons AS, Boer S, Uezato A, Meador-Woodruﬀ J, Scarr E, McCullumsmith RE. Changes
in cortical N-methyl-d-aspartate receptors and post-synaptic density protein 95 in schizophrenia, mooddisorders and suicide Aust N Z J Psychiatry . 2016; 50(3); 275-283
4. Akeo Kurumaji, Michio Itasaka, Akihito Uezato, Kazuo Takiguchi, Daisuke Jitoku, Mizue Hobo, Kiyotaka
Nemoto. A Reduced Gray Matter Volume in Patients with Bipolar II Disorder in a Japanese Sample: aComparison with Schizophrenia Bipolar Disorder: Open Access. 2016; 2(2);5. Nishida M, Nakashima Y, Nishikawa T. Slow sleep spindle and procedural memory consolidation inpatients with major depressive disorder. Nat Sci Sleep. 2016.01; 8; 63-72
6. Okumura Y, Hatta K, Wada K, Takeuchi T, Kishi Y; DELIRIA-J Group. Expert opinions on the ﬁrst-line
pharmacological treatment for delirium in Japan: a conjoint analysis Int Psychogeriatr. 2016.06; 28(6);1041-1050
7. Yoshiike T, Nishida M, Yagishita K, Nariai T, Ishii K, Nishikawa T. AlteredSleep Spindles in Delayed
Encephalopathy after Acute Carbon Monoxide Poisoning. J Clin Sleep Med. 2016.06; 12(6); 913-915
8. Balu DT, Li Y, Takagi S, Presti KT, Ramikie TS, Rook JM, Jones CK, Lindsley CW, Conn PJ, Bolshakov
VY, Coyle JT. An mGlu5-Positive Allosteric Modulator Rescues the Neuroplasticity Deﬁcits in a Genetic
Model of NMDA Receptor Hypofunction in Schizophrenia. Neuropsychopharmacology. 2016.08; 41(8);2052-2061
9. Ando K, Soshi T, Nakazawa K, Noda T, Okada T. Risk Factors for Problematic Behaviors among Forensic
Outpatients under the Medical Treatment and Supervision Act in Japan. Front Psychiatry. 2016.08; 7;
144
10. Ichikura K, Okumura Y, Takeuchi T. Associations of adverse clinical course and ingested substances among
patients with deliberate drug-poisoning: a cohort study from an intensive care unit in Japan PLOS ONE.
2016.08; 11(8);― 240 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine[Books etc]

1. Nishikawa T．Eds: Yoshimura T, Nishikawa T and Homma H. D-Serine signaling and schizophrenia,
Chapter 6, Part II Physiological Functions and Pathophysiology of D-Serine , In D-Amino AcidsPhysiology, Metabolism, and Application- ．. Springer, Tokyo, 2016
2. Nishikawa T．Eds: Yoshimura T, Nishikawa T and Homma H. Overview, Chapter 2, Part II Physiological Functions and Pathophysiology of D-Serine , In D-Amino Acids-Physiology, Metabolism, andApplication- ．. Springer, Tokyo, 2016
[Conference Activities & Talks]
1. Masashi Ohta, Masahito Nakataki , Mai Tamaru, Hiroko Kubo, Chiaki Nakayama, Yasuhiro Funakoshi,
Akihito Uezato, Shusuke Numata, Jun-ichi Iga, Satsuki Sumitani, Masafumi Harada, Toru Nishikawa,
Tetsuro Ohmori. Functional connectivity of the medial prefrontal cortex in schizophrenia patients treatedwith D-cycloserine: A resting state functional MRI study. 46th Annual Meeting of the Japanese Societyof Neuropsychopharmacology. 2016.07.02 Seoul
2. Sayuri Ishiwata, kotaro Hattori, Daimei sasayama, Toshiya teraishi, Tomoko Miyakawa, Yuuki Yokota,
Ryo Matsumura, Toru Nishikawa, Hiroshi Kunugi. Evaluation of plasma and cerebrospinal ﬂuid G72
protein levels and their correlations with psychiatric symptoms in schizophrenia and major depression.
30th CINP World congress of Neuropsychopharmacology 2016.07.04 Seoul
3. Yuichiro Abe. IPSRT- a sleep psychotherapeutic intervention based on social zeitgeber theory of bipolar
disorder.. Japanese Society of Sleep Research The 41st Annual Meeting InternationalForum 2016.07.09
Tokyo
4. Watanabe K，Ono S，Wachi T，Yokota K，Otsuka Y，Hirama K，Ando K，Okada T. Preliminary development and validity assessment of a culture-free version of the NRIPS and NCNP s Forensic Ability
Screening Test(N2-FAST). The 31st International Congress of Psychology (ICP2016) 2016.07.24 Yokohama, Kanagawa (PACIFICO Yokohama)
5. Okada T. Indiscriminate Mass Murders in Japan. The 17th Paciﬁc Rim College of Psychiatrists (PRCP)
Scientiﬁc Meeting 2016.11.03 Kaohsiung, Taiwan (Kaohsiung Exhibition Center)
6. A. UEZATO, N. YAMAMOTO, D. JITOKU, S. HIRAOKA, E.HARAMO, E. HIRAAKI, Y. IWAYAMA,
T. TOYOTA, M. UMINO, A. UMINO, Y.IWATA, K. SUZUKI, M. KIKUCHI, T. HASHIMOTO, N.
KANAHARA, A. KURUMAJI, T.YOSHIKAWA, T. NISHIKAWA. Genetic Association between DLG1
gene and schizophrenia. Neuroscience 2016 2016.11.15 San Diego
7. Okada T. Social Safety Nets - Crime Prevention, Correction and Victim Protection of Random Killing
Spree cases. 2016 Judicial Protection and Crime Prevention Forum: Strengthen Cooperation and Future
Challenges 2016.12.16 Tapiei, Taiwan (Hsinchuang United Oﬃce Building, Exective Yuan)[Awards & Honors]
1. JSNP Excellent Presentation Award for CINP2016 (Sayuri Ishiwata), The Japanese Society of Neuropsychopharmacology, 2016.07― 241 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

Neurosurgery

Professor: Taketoshi Maehara
Associate Professor: Tadashi Nariai
Assistant Professors: Yoji Tanaka and Motoki Inaji
Hospital stuﬀs:Takashi Sugawara, Kaoru Tamura,Kazutaka Sumita and Takumi Kudo,
Graduate Students: Masahumi Sasaki, Yoshiteru Obata,Yousuke Ishii, Sakyo Hirai, Yasuhiro Ueda, Jun Karakama, Takahiro Ogishima, Shihori Hayashi, Kazuhide Shimizu, Don(1)

OutlineThere are various attracting subjects in the ﬁeld of clinical or basic research. It is essential to acquire thesuﬃcient knowledge and insight into the pathological conditions as well as normal functions of the central
nervous system and spinal cord, which will directly beneﬁt for the improvement of clinical results. Main
educational purpose of neurosurgery in the graduate course is to provide students opportunity to acquire theproper technique as well as the broad knowledge, and to nurture the mind of exploration.
In the clinical practice, it is important to attach priority to the patients, considering their background. Also in
surgery, it is important to preserve the normal brain functions by employing the cutting edge technique. In the
research ﬁeld, it is essential to introduce and develop the latest knowledge and technology by establishing thereciprocal relationship with the other laboratory institutions.
(2)

Research

Brain tumors
1. Analysis of the mechanism of tumor proliferation and inﬁltration, and its application to treatment
2. Analysis of both proliferative and inhibitory cancer genes in cerebral and spinal tumors
3. Studies of photodynamic therapy, irradiation therapy, agents of chemotherapy, immunotherapy, and inhibitionof angiogenesis
4. Development of the multi-modal navigation system integrated with anatomical, hemodynamic, and functional
information for brain tumor surgery and evaluate its eﬃcacy.
Vascular diseases in the central nervous system and spinal cord
1. Analysis of pathogenesis of vasospasm after subarachnoid hemorrhage and its application to treatment
2. Studies of circulatory disturbance in ischemic and hemorrhagic diseases, and reversibility of the brain tissue
3. Investigations of pathology of Moyamoya disease and the eﬀects of indirect surgical anastomosis on thisentity4. Solutions of problems in the development of endovascular surgery
Neurotrauma
1. Analysis of cell damage and its reversibility, dynamic simulation in cerebrospinal injury
2. Animal experiments concerning treatment of cerebrospinal injury
Functional neurosurgery
1. Pathological analysis and treatment of temporal lobe epilepsy
2. Analysis of intracellular signal transductions― 242 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

Others
1. Studies of human cerebral circulation, metabolism, and functions using PET, MRI/S, and MEG
2. Studies of receptors in the central nervous system using PET
3. Experiments of brain diseases using animal model MRI and PET(3)Clinical Services & Other Works

Neurosurgery is a clinical department dealing with various diseases of central nervous system and spinal cord
including tumors, vascular diseases, trauma, congenital malformation, functional disorders, and infection.
(4)

Publications[Original Articles]
1. Furuse M, Nonoguchi N, Kuroiwa T, Miyamoto S, Arakawa Y, Shinoda J, Miwa K, Iuchi T, Tsuboi K,
Houkin K, Terasaka S, Tabei Y, Nakamura H, Nagane M, Sugiyama K, Terasaki M, Abe T, Narita Y,
Saito N, Mukasa A, Ogasawara K, Beppu T, Kumabe T, Nariai T, Tsuyuguchi N, Nakatani E, Kurisu
S, Nakagawa Y, Miyatake SI.. A prospective, multicentre, single-arm clinical trial of bevacizumab forpatients with surgically untreatable, symptomatic brain radiation necrosisdagger. Neurooncol Pract.
2016; 3; 272-280
2. Sugawara T, Maehara T, Nariai T, Aoyagi M, Ohno K. Independent predictors of shunt-dependent normalpressure hydrocephalus after aneurysmal subarachnoid hemorrhage. Journal of neurosurgical sciences..
2016; 60; 154-158
3. Atsunobu Tsunoda, Yoji Tanaka, Takanobu Sato, Susumu Kirimura, Ken Kitamura. Vertigo by Breast
Cancer Metastasis 33 Years after Treatment. Intern. Med.. 2016; 55(3); 311-313
4. Miho Watanabe, Haiying Li, Aimee G Kim, Aaron Weilerstein, Anteneta Radu, Marcus Davey, Stavros
Loukogeorgakis, Melissa D Sánchez, Kazutaka Sumita, Naoki Morimoto, Masaya Yamamoto, Yasuhiko
Tabata, Alan W Flake. Complete tissue coverage achieved by scaﬀold-based tissue engineering in the fetal
sheep model of Myelomeningocele. Biomaterials. 2016.01; 76; 133-143
5. Nobuo Sanjo,Satoko Kina,Yukiko Shishido-Hara,Yurie Nose,Satoru Ishibashi,Tetsuya Fukuda,Taketoshi
Maehara,Yoshinobu Eishi,Hidehiro Mizusawa,Takanori Yokota. A Case of Progressive Multifocal Leukoencephalopathy with Balanced CD4/CD8 T-Cell Inﬁltration and Good Response to Meﬂoquine Treatment.
Internal Medicine. 2016.01; 55(12); 1631-1635
6. Kazutaka Sumita, Yu-Hua Lo, Koh Takeuchi, Miki Senda, Satoshi Kofuji, Yoshiki Ikeda, Jumpei Terakawa, Mika Sasaki, Hirofumi Yoshino, Nazanin Majd, Yuxiang Zheng, Emily Rose Kahoud, Takehiro
Yokota, Brooke M Emerling, John M Asara, Tetsuo Ishida, Jason W Locasale, Takiko Daikoku, Dimitrios
Anastasiou, Toshiya Senda, Atsuo T Sasaki. The Lipid Kinase PI5P4K β Is an Intracellular GTP Sensor
for Metabolism and Tumorigenesis. Mol. Cell. 2016.01; 61(2); 187-198
7. Katsushige Sato, Shihori Hayashi, Motoki Inaji, Yoko Momose-Sato. Oscillations in the embryonic chickolfactory bulb: initial expression and development revealed by optical imaging with a voltage-sensitivedye. Eur. J. Neurosci.. 2016.02;
8. T Nakamura, K Tateishi, T Niwa, Y Matsushita, K Tamura, M Kinoshita, K Tanaka, S Fukushima, H
Takami, H Arita, A Kubo, T Shuto, M Ohno, Y Miyakita, S Kocialkowski, T Sasayama, N Hashimoto, T
Maehara, S Shibui, T Ushijima, N Kawahara, Y Narita, K Ichimura. Recurrent mutations of CD79B and
MYD88 are the hallmark of primary central nervous system lymphomas. Neuropathol. Appl. Neurobiol..
2016.04; 42(3); 279-290
9. Mukawa, M. Nariai, T. Inaji, M. Tamada, N. Maehara, T. Matsushima, Y. Ohno, K. Negi, M. Kobayashi,
D.. First autopsy analysis of a neovascularized arterial network induced by indirect bypass surgery formoyamoya disease J Neurosurg. 2016.05; 124(5); 1211-1214― 243 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

10. Koichi Ichimura, Shintaro Fukushima, Yasushi Totoki, Yuko Matsushita, Ayaka Otsuka, Arata Tomiyama,
Tohru Niwa, Hirokazu Takami, Taishi Nakamura, Tomonari Suzuki, Kohei Fukuoka, Takaaki Yanagisawa, Kazuhiko Mishima, Yoichi Nakazato, Fumie Hosoda, Yoshitaka Narita, Soichiro Shibui, Akihiko
Yoshida, Akitake Mukasa, Nobuhito Saito, Toshihiro Kumabe, Masayuki Kanamori, Teiji Tominaga, Keiichi Kobayashi, Saki Shimizu, Motoo Nagane, Toshihiko Iuchi, Masahiro Mizoguchi, Koji Yoshimoto,
Kaoru Tamura, Taketoshi Maehara, Kazuhiko Sugiyama, Mitsutoshi Nakada, Keiichi Sakai, Yonehiro
Kanemura, Masahiro Nonaka, Akio Asai, Kiyotaka Yokogami, Hideo Takeshima, Nobutaka Kawahara,
Tatsuya Takayama, Masahiro Yao, Mamoru Kato, Hiromi Nakamura, Natsuko Hama, Ryuichi Sakai,
Toshikazu Ushijima, Masao Matsutani, Tatsuhiro Shibata, Ryo Nishikawa, . Recurrent neomorphic mutations of MTOR in central nervous system and testicular germ cell tumors may be targeted for therapy.
Acta Neuropathol.. 2016.06; 131(6); 889-901
11. Yoshiike T, Nishida M, Yagishita K, Nariai T, Ishii K, Nishikawa T.. Altered Sleep Spindles in Delayed
Encephalopathy after Acute Carbon Monoxide Poisoning. J Clin Sleep Med.. 2016.06; 12(6); 913-915
12. Hideyuki Arita, Kai Yamasaki, Yuko Matsushita, Taishi Nakamura, Asanao Shimokawa, Hirokazu Takami,
Shota Tanaka, Akitake Mukasa, Mitsuaki Shirahata, Saki Shimizu, Kaori Suzuki, Kuniaki Saito, Keiichi
Kobayashi, Fumi Higuchi, Takeo Uzuka, Ryohei Otani, Kaoru Tamura, Kazutaka Sumita, Makoto Ohno,
Yasuji Miyakita, Naoki Kagawa, Naoya Hashimoto, Ryusuke Hatae, Koji Yoshimoto, Naoki Shinojima,
Hideo Nakamura, Yonehiro Kanemura, Yoshiko Okita, Manabu Kinoshita, Kenichi Ishibashi, Tomoko
Shofuda, Yoshinori Kodama, Kanji Mori, Yusuke Tomogane, Junya Fukai, Koji Fujita, Yuzo Terakawa,
Naohiro Tsuyuguchi, Shusuke Moriuchi, Masahiro Nonaka, Hiroyoshi Suzuki, Makoto Shibuya, Taketoshi
Maehara, Nobuhito Saito, Motoo Nagane, Nobutaka Kawahara, Keisuke Ueki, Toshiki Yoshimine, Etsuo Miyaoka, Ryo Nishikawa, Takashi Komori, Yoshitaka Narita, Koichi Ichimura. A combination of
TERT promoter mutation and MGMT methylation status predicts clinically relevant subgroups of newly
diagnosed glioblastomas. Acta Neuropathol Commun. 2016.08; 4(1); 79
13. Maki Mukawa, Tadashi Nariai, Hideaki Onda, Taku Yoneyama, Yasuo Aihara, Kengo Hirota, Takumi
Kudo, Kazutaka Sumita, Taketoshi Maehara, Takakazu Kawamata, Hidetoshi Kasuya, Hiroyuki Akagawa. Exome Sequencing Identiﬁed CCER2 as a Novel Candidate Gene for Moyamoya Disease. J Stroke
Cerebrovasc Dis. 2016.10;
14. Shoko Hara, Osamu Tone, Mutsuya Hara, Yohei Sato, Hideko Hashimoto, Akihiro Watanabe, Satoshi
Kaneko, Satoka Hashimoto, Masashi Tamaki. Postoperative Chronic Subdural Hematoma Following
Unruptured Cerebral Aneurysm Surgery Surgery for Cerebral Stroke. 2016.11; 44(6); 453-460
15. Shin Hirota, Motoki Inaji, Tadashi Nariai, Mutsuya Hara, Masashi Tamaki, Taketoshi Maehara, Hiroki
Tomita, Osamu Tone. Correlations between Cognitive Impairments and Employment Status in Patientswith Diﬀuse Axonal Injury. Neurol. Med. Chir. (Tokyo). 2016.12;
16. Masahiro Kishikawa, Atsunobu Tsunoda, Yoji Tanaka, Seiji Kishimoto. Large nasopharyngeal inverted
papilloma presenting with rustling tinnitus. Am J Otolaryngol. 35(3); 402-404[Books etc]
1. Tadashi Nariai. Moyamoya Disease. 2016.01[Conference Activities & Talks]
1. Shoko Hara, Yoji Tanaka, Motoki Inaji, Kenji Ishii, Taketoshi Maehara, Masaaki Hori, Shigeki Aoki andTadashi Nariai. non-invasive evaluation of CBF, perfusion delay and microstructure of moyamoya diseaseusing new analysis techniques of ASL and diﬀusion MRI. The 45th Annual Meeting of The JapaneseSociety of Neuroradiology 2016.02.20 Yamagata, Japan
2. Nariai, T. . Early amelioration of hemodynamics of moyamoya disease by indirect bypass surgery.. Seminarat PLA hospital 2016.02.29 Beijing
3. Nariai, T. . Educational Course: Neuroimaging with PET/MRI. Brain. Perfusion & Permeability Imaging.. ISMRM 2016 2016.05.07 .Singapore.

― 244 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

4. Y. Ishii, T. Nariai, Y. Tanaka, M. Yamashina, H. Satoh, S. Wakabayashi ,Y. Suyama, H. Aihara, H.
Kajikawa, T. Maehara.. Chronological Evaluation of Cerebral Hemodynamics by Dynamic Susceptibility
Contrast Magnetic Resonance Imaging after Indirect Bypass Surgery for Moyamoya Disease. ISMRM
2016 2016.05.11 Singapore
5. Tamura, K., Aoyagi, M., Dong, X., Kobayashi, D., Ando, N., Ogishima, T., Yamamoto, M., Maehara,
T.. Involvement of COX2, PGE2 and VEGF in vestibular schwannoma growth and cyst formation after
gamma knife stereotactic radiosurgery. The 34rd Annual Meeting of the Japan Society of Brain Tumor
Pathology 2016.05.27 Tokyo
6. Takashi Sugawara,
Masaru Aoyagi,
Masashi Tamaki,
Yano Tomoyuki,
Atsunobu Tsunoda,
Kikuo Ohno,
Taketoshi Maehara,
Seiji Kishimoto. Extended Orbital Exenteration for Sinonasal
Malignancy w/ Orbital Apex Extension. 7th International Congress of the World Federation of Skull
Base Societies 2016.06.16 Osaka
7. Takashi Sugawara, MD , PhD, Masaru Aoyagi, MD , PhD, Takahiro Ogishima, MD, Masashi Tamaki, MD, PhD, Tomoyuki Yano, MD , Atsunobu Tsunoda, MD , PhD, Kikuo Ohno, MD , PhD, Taketoshi Maehara,
MD , PhD, and Seiji Kishimoto, MD , PhD. Extended orbital exenteration for sinonasal malignancy withorbital apex extension: surgical technique and clinical analysis. 28th Annual Meeting of Japanese Society
for Skull Base Surgery 2016.06.16 Osaka
8. Tadashi Nariai. . Progress and remaining questions on surgical treatment of juvenile patients withmoyamoya disease. . 8th European-Japanese Cerebrovascular Congress 2016.06.24 Zurich. Switzerland
9. Tadashi Nariai.. Novel imaging technique for CBF and cerebrovascular autoregulation in patients withmoyamoya disease.. 8th European-Japanese Cerebrovascular Congress. 2016.06.25
10. Akitaka Muta, Mikio Hiura, Tadashi Nariai, Taketoshi Maehara, Muneyuki Sakata, Keiichi Oda, Jun
Toyohara, Kiichi Ishiwata、Kenji Ishii. Regional cerebral blood ﬂow during post exercise hypotension
following a single bout aerobic exercise examined by PET and TCD.. OHBM2016 2016.06.29 Geneve
11. Shoko Hara, Yoji Tanaka, Ayana Sawayanagi, Daisu Abe, Yasuhiro Ueda, Shihori Hayashi, Motoki Inaji,
Kenji Ishii, Taketoshi Maehara, Tadashi Nariai. The meaning of visual assessment of ASL-MRI may diﬀer
between moyamoya disease and atherosclerotic cereblovascular disease. The 75th Annual Meeting of theJapan Neurosurgical Society 2016.09.29 Fukuoka, Japan
12. Satoshi Kaneko, Takashi Sugawara, Kazutaka Sumita, Motoki Inaji, Yoji Tanaka, Tadashi Nariai, Daisuke
Kobayashi, Taketoshi Maehara. The outcome and prognosis of malignant meningiomas in our faculty. The
75th Annual Meeting of the Japan Neurosurgical Society 2016.09.29 Fukuoka, Japan
13. Kei Ito, Kaoru Tamura, Daisuke Kobayashi, Kazutaka Sumita, Msashi Tamaki, Taketoshi Maehara. Long
term survivors with glioblastoma; clinical and pathological analysis. The 75th Annual Meeting of theJapan Neurosurgical Society 2016.09.29 Fukuoka, Japan
14. Hara S, Nariai T, Murata S, Tsuruta K, Tanaka Y, Hori M, Maehara T, Aoki S. Neurite orientation
dispersion and density imaging revealed the brain microstructural ischemic damage of moyamoya disease.
Neuroscience 2016 2016.11.15 San Diego, Calﬁronia, U.S.
15. Shoko Hara, Masaaki Hori, Syo Murata, Ryo Ueda, Misaki Nakazawa, Yoji Tanaka, Taketoshi Maehara,
Shigeki Aoki, and Tadashi Nariai.. Neurite Orientation Dispersion and Density Imaging revealed the brain
microstructural ischemic damage in patients with moyamoya disease.. SfN2016 2016.11.15 San Diego
16. Kei Ito, Kaoru Tamura, Daisuke Kobayashi, Msashi Tamaki, Kazutaka Sumita, Taketoshi Maehara. Long
term survivors with glioblastoma. The 34th Annual Meeting of the Japan Society for Neuro-oncology
2016.12.04 Koufu, Japan― 245 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine

Endovascular Surgery

Professor Shigeru Nemoto
Associate Professor Yoshikazu Yoshino
Assistant Professor Kazunori Miki
Clinical Fellow Jun Karakama
Secretary Yoko Yanagida, Hitomi Kuwahara(1)

OutlineThere are various attracting subjects in the ﬁeld of clinical or basic research. It is essential to acquire the suﬃcient knowledge and insight into the pathological conditions as well as normal functions of the vascular system,
which will directly beneﬁt for the improvement of clinical results. Main educational purpose of Endovascular
Surgery in the graduate course is to provide physicians/students opportunity to acquire the proper techniqueas well as the broad knowledge, and to nurture the mind of exploration.

(2)

Research

Our experimental research program is objected to elucidate unsolved questions derived from daily clinicalexperience. To treat vascular diseases of central nervous system, facial and head-neck legions, we need tounderstand detailed vascular anatomy, accurate function of these organs and exact pathophysiology of eachdisease. Our essential research target is the hemodynamics in the vascular diseases of these lesions. Especially
we are interested in the integration of the ﬂuid engineering technology into the endovascular ﬁeld in an eﬀort
to open a new frontier of surgical treatment.

(3)

Education

Course objects of Endovascular Surgery in the graduate course is to acquire the proper technique as well as thebasic knowledge of neuroendovascular surgery.

(4)

Lectures & Courses

Main educational purpose of Endovascular Surgery in the graduate course is to provide students the proper
technique as well as the basic knowledge of neuroendovascular surgery.

(5)

Clinical Services & Other Works

Our major clinical and extracurricular activities are as follows. 1. Endovascular surgery for diseases of central
nervous system, facial and head-neck legions. 2. Analysis of cerebrovascular diseases using computational ﬂuid
dynamics (CFD). 3. Development of integrated training system for the endovascular surgery.

― 246 ―

Cognitive and Behavioral Medicine

Cognitive and Behavioral Medicine(6)

Clinical Performances

Endovascular Surgery is a clinical department dealing with various vascular diseases of central nervous system,
spinal cord, facial and head-neck lesions including tumors, congenital malformation, and functional disorders.
(7)

Publications[Conference Activities & Talks]
1. Shigeru Nemoto. Characteristics of stent retriever on removing clot —bench test using artiﬁcial clot andendovascular simulation system.. AAFITN
12th Asian Australasian Federation of Interventional andTherapeutic Neuroradiology 2016.03.24
2. Evaluating the eﬀect of intra arterial fasudil injection for vasospasm of subarachnoid hemorrhage using
the novel angiographic analysis.. 2016.04.11
3. The role and controversy of adjunctive technique for cerebral aneurysm coil embolization. . 2016.04.16
4. Shigeru Nemoto. IC occlusion immediately after coil embolization of Pcom aneurysm.. CerebrovascularComplications Conference (3C) 2016.06.14 Jacksonhole，Wyoming
5. Shigeru Nemoto. 3D vascular model is valuable in neurointervention.. ESNR （European Society of
Neuroradiology） 39th annual meeting 2016.09.15 Beograd,Serbia
6. Eﬃcacy of standard endovascular treatment for paraclinoid aneurysms. 2016.10.01
7. Shigeru Nemoto. Multiple Enterprise stents for vertebral aneurysms.. Siemen Advisory Meeting. 2016.11.05
Tokyo RihgaRoyalHotels
8. Endovascular Revascularization for Chronic Total Occlusion of the Internal Carotid Artery.. 2016.11.24
9. Eﬃcacy of standard endovascular treatment for paraclinoid aneurysms. 2016.11.26― 247 ―

Cognitive and Behavioral Medicine

NCNP Brain Physiology and Pathology
1. Staffs and Students
Collaborative Professor

Mikio HOSHINO

Collaborative Professor

Yu-ichi GOTO

Collaborative Professor

Hiroshi KUNUGICollaborative Professor

Manabu HONDA

Collaborative Professor

Noritaka ICHINOHE

Collaborative Associate Professor

Yoshitsugu AOKI(1)Research
1) Investigation of the molecular machinery underlying brain development.
(Mikio Hoshino; Department of Biochemistry and Cellular Biology, National Instituteof Neuroscience, NCNP)
We are investigating molecular machinery underlying nervous system development,
especially focusing on neuron-subtype specification, nervous system regionalization andneuronal migration. We are also interested in human diseases/disorders caused bydisorganized development of the nervous system.
The AUTS2 (Autism susceptibility candidate 2) gene has been related to various
psychiatric disorders, such as ASD (autism spectrum disorders), schizophrenia, ADHD,
and epilepsy. We generated mouse model in which AUTS2 gene was disrupted andanalyzed its behavior. We also investigated molecular machinery to specify excitatory
and inhibitory neurons in the cerebellum and climbing fiber neurons in the hindbrain.
2) Molecular genetic and genomic study for intellectual disability in Japan.
(Yu-ichi Goto, Department of Mental Retardation and Birth Defect Research, National
Institute of Neuroscience, NCNP)
One of the major causes of intellectual disability (ID) is based on mutations in therelated genes, which are timely and locally expressed in concert with one another incentral nervous system. ID is a phenotype derived from the inappropriate expression ofthese genes. Recent advances in molecular genetics and genome medicine have pushed
us on with systematic analysis of ID patients, especially on X-linked RQHV. In2013, weinvestigated the genetic causes and pathophysiology of mitochondrial disease, Rett
syndrome, and disease with cortical and white matter dysplasia. We found a uniqueeffect of reactive oxygen species to alteration of heteroplasmy (Ling P, et al. Mol Biol

― 248 ―

Cognitive and Behavioral Medicine

Cell 2016), GLIIHUHQWLDO blocking against nerve and cardiac commitment of iPS cells by
mitochondrial respiratory dysfunction㸦Yokota M, et al. Cell Death Dis 2017㸧a nd we
studied knock-out mice of Igfbp3 that is downstream of mecp2 associated with Rett
syndrome (Dai H, et al. Am J Pathol 2017).
3) Clinical research on mood disorders and schizophrenia(Hiroshi Kunugi, Department of Mental Disorder Research, National Institute of
Neuroscience, NCNP)
The pathogenesis and physiology of mood disorders and schizophrenia remain elusive,
and their biomarkers have not yet been established. Our department, which is incollaboration with the National Center of Neurology and Psychiatry Hospital, is trying
to develop objective diagnostic markers for these diseases, employing omics approach,brain imaging, and physiological studies. We also aim to develop new treatment on thebasis of key molecules and nutrition. In this year, we published genome-wide database
regulating protein levels in the cerebrospinal fluid (Hun Mol Genet, 2016), metabolome
finding on postmortem schizophrenia samples (Schizophr Res, 2016), the effect of ketogenic diet on
cognitive functions in elderly subjects (Psychopharmacology, 2016), reduced bacterial counts ofBifidobacterium and Lactobacillus in major depression (J Affect Disord, 2016).
4) Noninvasive study on pathophysiology of human higher brain function.
(Manabu Honda, Department of Functional Brain Research, National Institute of
Neuroscience, NCNP)
We try to reveal various human higher brain functions including sensory, motor,
thought, emotion and KANSEI functions and pathophysiology underlying higher brain
function disorders by integrating multiple noninvasive brain imaging techniques. We
also pursue researches for developing a new non-pharmacological therapy by means of
the hypersonic effect, that is, sounds containing rich high-frequency component of air
vibration above the human audible range activate the deep-lying brain structures
including midbrain and diencephalon.
In 2016, as basic researches, we showed that the natural life span of mice prolonged by
the enrichment of acoustic environment. As clinical researches, we developed the audio
system with ultra-wide rage and wide directivity available for clinical use and examined
effects of hypersonic sounds on clinical symptoms of BPSD using the originallydeveloped audio system with ultra-wide rage and wide directivity.
5) Primate Social brains: their development, anatomy, physiology and patho-physiology.

― 249 ―

Cognitive and Behavioral Medicine(Noritaka Ichinohe, Department of Ultrastructural Research, National Institute of
Neuroscience, NCNP)
We are aiming to elucidate the neural circuit mechanisms of primate social brain
using Common Marmoset, new primate model animal. Emphases are on theirdevelopment, anatomy, physiology and patho-physiology.
This year, we studied mechanisms of material perception, especially glossy materials
at medial temporal sulcus (Miyawaka and Banno et al., Front Neural Circuits, 2017).
6) Molecular pathogenesis and therapies for neuromuscular diseases(Yoshitsugu Aoki, Department of Molecular Therapy, National, National Institute of
Neuroscience, NCNP)
Our research group focuses on genetic neuromuscular diseases including fatal
Duchenne muscular dystrophy (DMD) and amyotrophic lateral sclerosis (ALS). Our lab
integrates molecular, pharmacologic, proteomic and genomic methodologies to clarify
the molecular mechanisms of disease pathogenesis and develop novel genetic therapies
for

the

diseases.

Especially,

we

are

dedicatedtothedevelopmentofantisense-oligonucleotides based drugs for the diseases in collaboration with Profs.
Matthew Wood, Oxford, Kevin Talbot, Oxford, Toshifumi Yokota, Alberta, Fazel
Shabanpoor, Melbourne and Samir El-Andaloussi, Karolinska Institutet.
During the academic year 15/16, we have discovered a fundamentally disturbed
molecular pathway in C9orf72-related ALS and frontotemporal dementia that identifies
a novel extracellular vesicle trafficking, determines the molecular mechanisms and forthe first time links ALS pathogenesis with extracellular vesicle biogenesis (Aoki et al.,
BRAIN, 2017). The goal of the laboratory is a better understanding and improved
treatment of fatal and currently untreated neuromuscular diseases.

(2) Education
The nervous system is a very fine and complex organ to elicit the higher brain
function and its malfunction causes a variety of neurological and psychiatric disorders
in humans. In this course, students learn the structure, development and function of
the normal nervous and muscle systems as well as pathology of developmental disorders,
psychiatric disorders, neurological diseases and muscle diseases. Students also study
the latest progress of advanced remedy for neuromuscular diseases.

(3) Publications― 250 ―

Cognitive and Behavioral Medicine[Original Articles]
1)

Hanai S, Sukigara S, Dai H, Owa T, Horike S, Otsuki T, Saito T, Nakagawa E,
Ikegaya N, Kaido T, Sato N, Takahashi A, Sugai K, Saito Y, Sasaki M, Hoshino M,
Goto Y, Koizumi S, Itoh M. Pathologic active mTOR mutation in brain
malformation with intractable epilepsy leads to cell-autonomous migration delay.
American Journal of Pathology, in press

2)

Ling P, Rong N, Hatakeyama H, Goto Y, Shibata T, Yoshida M. Reactive oxygen
species stimulate mitochondrial allele segregation toward homoplasmy in humancells. Mol Biol Cell 27(5): 1684-1693, 20163)

Yokota M, Hatakeyama H, Ono Y, Kanazawa M, Goto Y. Mitochondrial respiratory
dysfunction disturbs neuronal and cardiac lineage commitment of human iPSCs.
Cell Death Dis 8: e2551, 20174)

Dai H, Goto Y, Itoh M. Insulin-like growth factor binding protein-3 deficiency leads
to behavior impairment with monoaminergic and synaptic dysfunction. Am J
Pathol 187(2): 390-400, 20175)

Kuroki N, Kashiwagi H, Ota M, Ishikawa M, Kunugi H, Sato N, Hirabayashi N,
Ota T. Brain structure differences among male schizophrenic patients with historyof serious violent acts: an MRI voxel-based morphometric study. BMC Psychiatry.
2017;17(1):105.

6)

Hidese S, Hattori K, Sasayama D, Miyakawa T, Matsumura R, Yokota Y, Ishida I,
Matsuo J, Noda T, Yoshida S, Teraishi T, Hori H, Ota M, Kunugi H. Cerebrospinal
fluid neural cell adhesion molecule levels and their correlation with clinicalvariables in patients with schizophrenia, bipolar disorder, and major depressive
disorder. Prog Neuropsychopharmacol Biol Psychiatry. 2017;76:12-18.

7)

Ikeda M, Takahashi A, Kamatani Y, Okahisa Y, Kunugi H, Mori N, Sasaki T,
Ohmori T, Okamoto Y, Kawasaki H, Shimodera S, Kato T, Yoneda H, Yoshimura R,
Iyo M, Matsuda K, Akiyama M, Ashikawa K, Kashiwase K, Tokunaga K, Kondo K,
Saito T, Shimasaki A, Kawase K, Kitajima T, Matsuo K, Itokawa M, Someya T,
Inada T, Hashimoto R, Inoue T, Akiyama K, Tanii H, Arai H, Kanba S, Ozaki N,
Kusumi I, Yoshikawa T, Kubo M, Iwata N. A genome-wide association study
identifies two novel susceptibility loci and trans population polygenicity associatedwith bipolar disorder. Mol Psychiatry. 2017 Jan 24.

8)

Matsuo J, Ota M, Hidese S, Hori H, Teraishi T, Ishida I, Hiraishi M, Kunugi H.
Sexually dimorphic deficits of prepulse inhibition in patients with major depressive
disorder and their relationship to symptoms: A large single ethnicity study. J Affect
Disord. 2017;211:75-82.

― 251 ―

Cognitive and Behavioral Medicine

9)

Sasayama D, Hattori K, Ogawa S, Yokota Y, Matsumura R, Teraishi T, Hori H, Ota
M, Yoshida S, Kunugi H. Genome-wide quantitative trait loci mapping of thehuman cerebrospinal fluid proteome. Hum Mol Genet, in press

10) Ota M, Sato N, Hidese S, Teraishi T, Maikusa N, Matsuda H, Hattori K, Kunugi H.
Structural differences in hippocampal subfields among schizophrenia patients,
major depressive disorder patients, and healthy subjects. Psychiatry Res.
2017;259:54-59.
11) Kasahara T, Ishiwata M, Kakiuchi C, Fuke S, Iwata N, Ozaki N, Kunugi H, Minabe
Y, Nakamura K, Iwata Y, Fujii K, Kanba S, Ujike H, Kusumi I, Kataoka M, Matoba
N, Takata A, Iwamoto K, Yoshikawa T, Kato T. Enrichment of deleterious variantsof mitochondrial DNA polymerase gene (POLG1) in bipolar disorder. Psychiatry
Clin Neurosci, in press
12) Setoyama D, Kato TA, Hashimoto R, Kunugi H, Hattori K, Hayakawa K,
Sato-Kasai M, Shimokawa N, Kaneko S, Yoshida S, Goto YI, Yasuda Y, Yamamori
H, Ohgidani M, Sagata N, Miura D, Kang D, Kanba S. Plasma Metabolites Predict
Severity of Depression and Suicidal Ideation in Psychiatric Patients-A Multicenter
Pilot Analysis. PLoS One. 2016;11(12):e0165267.
13) Ninomiya-Baba M, Matsuo J, Sasayama D, Hori H, Teraishi T, Ota M, Hattori K,
Noda T, Ishida I, Shibata S, Kunugi H. Association of body mass index-related
single nucleotide polymorphisms with psychiatric disease and memory performancein a Japanese population. Acta Neuropsychiatr, in press
14) Fujii T, Hattori K, Miyakawa T, Ohashi Y, Sato H, Kunugi H. Metabolic profile
alterations in the postmortem brains of patients with schizophrenia using capillaryelectrophoresis-mass spectrometry. Schizophr Res, in press
15) Ota M, Matsuo J, Ishida I, Hattori K, Teraishi T, Tonouchi H, Ashida K, Takahashi T, Kunugi
H. Effect of a ketogenic meal on cognitive function in elderly adults: potential for cognitiveenhancement. Psychopharmacology (Berl). 2016;233(21-22):3797-3802.
16) Yoshimura A, Kawamata M, Yoshioka Y, Katsuda T, Kikuchi H, Nagai Y, Adachi N,
Numakawa T, Kunugi H, Ochiya T, Tamai Y. Generation of a novel transgenic rat
model for tracing extracellular vesicles in body fluids. Sci Rep. 2016;6:31172.
17) Matsuo J, Ota M, Hori H, Hidese S, Teraishi T, Ishida I, Hiraishi M, Kunugi H. A
large single ethnicity study of prepulse inhibition in schizophrenia: Separate
analysis by sex focusing on effect of symptoms. J Psychiatr Res. 2016;82:155-62.
18) Ota M, Hori H, Sato N, Yoshida F, Hattori K, Teraishi T, Kunugi H. Effects ofankyrin 3 gene risk variants on brain structures in patients with bipolar disorderand healthy subjects. Psychiatry Clin Neurosci. 2016 Nov;70(11):498-506.

― 252 ―

Cognitive and Behavioral Medicine

19) Odaka H, Numakawa T, Yoshimura A, Nakajima S, Adachi N, Ooshima Y, Inoue T,
Kunugi H. Chronic glucocorticoid exposure suppressed the differentiation andsurvival of embryonic neural stem/progenitor cells: Possible involvement of ERK
andPI3K/Aktsignalingintheneuronaldifferentiation.

NeurosciRes.

2016;113:28-36.
20) Hidese S, Ota M, Wakabayashi C, Noda T, Ozawa H, Okubo T, Kunugi H. Effects ofchronic l-theanine administration in patients with major depressive disorder: an
open-label study. Acta Neuropsychiatr. 2016 Jul 11:1-8.
21) Aizawa E, Tsuji H, Asahara T, Takahashi T, Teraishi T, Yoshida S, Ota M, Koga N,
Hattori K, Kunugi H. Possible association of Bifidobacterium and Lactobacillus in
the gut microbiota of patients with major depressive disorder. J Affect Disord.
2016;202:254-7.
22) Yoshimura A, Numakawa T, Odaka H, Adachi N, Tamai Y, Kunugi H. Negative
regulation of microRNA-132 in expression of synaptic proteins in neuronaldifferentiation of embryonic neural stem cells. Neurochem Int. 2016;97:26-33.
23) Hori H, Koga N, Hidese S, Nagashima A, Kim Y, Higuchi T, Kunugi H. 24-h activity
rhythm and sleep in depressed outpatients. J Psychiatr Res. 2016;77:27-34.
24) Hori Y, Ogura J, Ihara N, Higashi T, Tashiro T, Honda M, Hanakawa T:
Development of a removable head fixation device for longitudinal behavioral andimaging studies in rats. J Neurosci Methods, 264: 11-15, 2016.
25) Yamashita Y, Fujimura T, Katahira K, Honda M, Okada M, and Okanoya K:
Context sensitivity in the detection of changes in facial emotion. Scientific Reports,
6: 27798-27798, 2016.
26) Miyakawa N, Banno T, Abe H, Tani T, Suzuki W, and Ichinohe N. Representation of
Glossy Material Surface in Ventral Superior Temporal Sulcal Area of Common
Marmosets. Front. Neural Circuits, 11, 17.2017.
27) Echigoya Y, Nakamura A, Nagata T, Urasawa N, Kuraoka M, Saito T, Aoki Y, Kole
R, Partridge T, Takeda S, Yokota T. Effects of systemic multiexon skipping withpeptide-conjugated morpholinos in the heart of a dog model of Duchenne muscular
dystrophy. Proc Natl Acad Sci U S A. 2017, in press.
28) Aoki Y, 㸦corresponding author㸧Manzano R, Lee Y, Aoki M, Takeda S, Fukuda M,
Talbot K, Wood MJA. C9ORF72 and RAB7L1 regulate vesicle trafficking in
Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. BRAIN. 23 Feb
2017.
29) Suzuki H, Aoki Y, Kameyama T, Saito T, Masuda S, Tanihata J, Nagata T, Mayeda
A, Takeda S, Tsukahara T. Endogenous Multiple Exon Skipping and Back-Splicing― 253 ―

Cognitive and Behavioral Medicine

at the DMD Mutation Hotspot. Int J Mol Sci. 2016 Oct 13;17(10).
30) Nakamura A, Fueki N, Shiba N, Motoki H, Miyazaki D, Nishizawa H, Echigoya Y,
Yokota T, Aoki Y, Takeda S. Deletion of exons 3-9 encompassing a mutational hot
spot in the DMD gene presents an asymptomatic phenotype, indicating a target
region for multiexon skipping therapy. J Hum Genet. 2016 Jul;61(7):663-7.
31) Kuraoka M, Kimura E, Nagata T, Okada T, Aoki Y, Tachimori H, Yonemoto N,
Imamura M, Takeda S. Serum Osteopontin as a Novel Biomarker for Muscle
Regenerationin

DuchenneMuscular

May;186(5):1302-12.

― 254 ―

Dystrophy.

Am

J

Pathol.

2016

Bio-Environmental
Bio-Environmental Response
Response

Immune Regulation

Professor Hajime Karasuyama, M.D., Ph.D.
Junior Associate Professor Yoshinori Yamanishi, M.D., Ph.D.
Assistant Professor Shingo Sato, Ph.D.
Assistant Professor Soichiro Yoshikawa, Ph.D.
(1)

Research

1) Role of basophils in immune disorders such as allergy2) Role of basophils in protective immunity against infections3) in vivo imaging of basophil-mediated immune responses(2)

Education

Main objective of the immunology course for undergraduate students is to provide them the basic ideas how theimmune system works and is regulated in various physiological and pathological settings including infections,cancer, autoimmune and allergic disorders, and organ transplantation. In the immunology course for graduate
students, they study molecular mechanisms underlying the development of immune diseases including allergy
and parasitic infection, by employing advanced technology in molecular biology, biochemistry, cellular biology
and developmental engineering.
(3)

Publications[Original Articles]
1. Adachi, T., Kakuta, S., Aihara, Y., Kamiya, T., Watanabe, Y., Osakabe, N., Hazato, N., Miyawaki, A.,
Yoshikawa, S., Usami, T., Karasuyama, H., Kimoto-Nira, H., Hirayama, K., M Tsuji, N.. Visualization of
Probiotic-Mediated Ca(2+) Signaling in Intestinal Epithelial Cells In Vivo. Front Immunol. 2016; 7; 601
2. Yoshikawa, S., Usami, T., Kikuta, J., Ishii, M., Sasano, T., Sugiyama, K., Furukawa, T., Nakasho, E.,
Takayanagi, H., Tedder, T. F., Karasuyama, H., Miyawaki, A., Adachi, T.. Intravital imaging of Ca(2+)
signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before thepathological onset. Sci Rep. 2016.01; 6; 18738
3. Matsukawa, T., Izawa, K., Isobe, M., Takahashi, M., Maehara, A., Yamanishi, Y., Kaitani, A., Okumura,K., Teshima, T., Kitamura, T., Kitaura, J.. Ceramide-CD300f binding suppresses experimental colitis by
inhibiting ATP-mediated mast cell activation. Gut. 2016.05; Epub ahead of print;
4. Baba, T., Tanabe, Y., Yoshikawa, S., Yamanishi, Y., Morishita, S., Komatsu, N., Karasuyama, H., Hirao,A., Mukaida N.. MIP-1 α/CCL3-expressing basophil-lineage cells drive the leukemic hematopoiesis ofchronic myeloid leukemia in mice. Blood. 2016.05; 127(21); 2607-2617
5. Schiechl, G., Hermann, F.J., Rodriguez Gomez, M., Kutzi, S., Schmidbauer, K., Talke, Y., Neumayer, S.,
Goebel, N., Renner, K., Brühl, H., Karasuyama, H., Obata-Ninomiya, K., K Utpatel, K., Evert, M., Hirt,
S.W., Geissler, E.K., Fichtner-Feigl, S., Mack, M.. Basophils Trigger Fibroblast Activation in Cardiac
Allograft Fibrosis Development. Am. J. Transplant.. 2016.09; 16(9); 2574-2588― 255 ―

Bio-Environmental Response
Response
Bio-Environmental

6. Horiguchi, K., Yoshikawa, S., Saito, A., Haddad, S., Ohta, T., Miyake, K., Yamanishi, Y., Karasuyama,
H.. Real-time imaging of mast cell degranulation in vitro and in vivo. Biochem. Biophys. Res. Commun..
2016.10; 479(3); 517-522
7. Kojima, T., Tsuchiya, K., Ikemizu, S., Yoshikawa, S., Yamanishi, Y., Watanabe, M., Karasuyama, H..
Novel CD200 homologues iSEC1 and iSEC2 are gastrointestinal secretory cell-specific ligands of inhibitory
receptor CD200R. Sci Rep. 2016.11; 6; 36457
8. Venturelli, N., Lexmond, W. S., Ohsaki, A., Nurko, S., Karasuyama, H., Fiebiger, E., Oyoshi, M. K..
Allergic skin sensitization promotes eosinophilic esophagitis through the IL-33-basophil axis in mice. J.
Allergy Clin. Immunol.. 2016.11; 138(5); 1367-1380.e5
9. Iki, M., Tanaka, K., Deki, H. Fujimaki, M., Sato, S., Yoshikawa, S., Yamanishi, Y., Karasuyama, K..
Basophil tryptase mMCP-11 plays a crucial role in IgE-mediated, delayed-onset allergic inflammation in
mice. Blood. 2016.12; 128(25); 2909-2918[Misc]
1. Yamanishi, Y., Karasuyama, H.. Basophils and mast cells in immunity and inflammation. Semin Immunopathol. 2016.09; 38(5); 535-537
2. Yamanishi, Y., Karasuyama, H.. Basophil-derived IL-4 plays versatile roles in immunity. Semin Immunopathol. 2016.09; 38(5); 615-622[Conference Activities & Talks]
1. Nagao, T., Takahashi, S., Kawawa, M., Miyake, K., Yoshikawa, S., Sato, S., Yamanishi, Y., Karasuyama,
H.. Molecular properties of carrier protein in allergen are critical for the development of IgE- and basophilmediated allergic inflammation in the skin.. Environment controlling normal and diseased hematopoetic
and immune systems 2016.03.02 Yokohama, Japan
2. Karasuyama, H.. Emerging roles of basophils and IgE in allergy and immunity.. FASEB Science Research
Conference 2016.07.26 Florida
3. Yoshikawa, S., Oh-hora, M., Adachi, T., Yamanishi, Y., karasuyama, H. . STIM1/2 plays essential rolein basophil activation and development of IgE-mediated chronic allergic inflammation. . International
Congress of Immunology 2016 2016.08.21 Melbourne, Autralia
4. Nagao, T., Takahashi, S., Kawawa, M., Miyake, K., Yoshikawa, S., Yamanishi, Y., Karasuyama, H.. Sugar
modification of carrier protein in allergen determines the magnitude of IgE- and basophil-mediated allergic
inflammation.. International Congress of Immunology 2016 2016.08.23 Melbourne, Autralia
5. Tsutsui, H., Yamanishi, Y., Yoshikawa, S., Sato, S., Karasuyama, H.. mMCP-8, a basophil specific protease, triggers an inflammatory response by stimulating fibroblasts to produce chemokines. The 16th International Congress of Immunology.. International Congress of Immunology 2016 2016.08.23 Melbourne,
Autralia
6. Karasuyama, H.. Emerging roles of basophils in acquired protective immunity to tick infestation.. XXVInternational Congress of Entomology 2016.09.29 Orland
7. Yoshikawa, S., Oh-hora, M., Yamanishi, Y., Karasuyama, H.. STIM2 plays essential role in cytokineinduced IL-4 production in basophil.. The 45th Annual Meeting of The Japanese Society for Immunology
2016.12.05 Okinawa, Japan
8. Iki, M., Tanaka, K., Deki, H., Nagao, T, Horibe, M., Sato, S., Yoshikawa, S., Yamanishi Y., Karasuyama,
H.. Basophil-selective mMCP-11 plays crucial roles in the development of IgE-mediated chronic allergic
inflammation in the skin.. The 45th Annual Meeting of the Japanese Society for Immunology 2016.12.06
Okinawa
9. Miyake, K., Shiozawa, N., Nagao, T., Kawawa, M., Yoshikawa, S., Yamanishi, Y., Karasuyama, H..
Basophils gain the capacity of antigen presentation by acquiring MHC class II molecules from dendritic
cells through trogocytosis.. The 45th Annual Meeting of the Japanese Society for Immunology 2016.12.06
Okinawa― 256 ―

Bio-Environmental
Bio-Environmental Response
Response

10. Karasuyama, H.. Emerging roles of basophils in allergy and immunity.. 41th JSID Annual Meeting2016.12.09 Sendai[Awards & Honors]
1. Best Presentation Award Winners 2016(The 45th Annual Meeting of the Japanese Society for Immunology)(Misako Iki), The Japanese Society for Immunology, 2016.12― 257 ―

Bio-Environmental Response

Bio-Environmental Response

Molecular Virology

Professor：Shoji YAMAOKA
Project Professor：Eiji IDO
Assistant Professor: Hiroaki TAKEUCHI,Takeshi YOSHIDA,
Momoe ITSUMI
Medical Technologist：Yoshio INAGAKISecretary：Kumiko THORPE-MATSUI-StudentsPh.D. course: Miho OHSAKO
Hideki SAITO
Hirona ICHIKAWA
Naoto SUZUKINDZINU JERRY KWAMEAZIATI ISHMAEL DZIGBDRDI KWASIMXWELL MAMFE SAKYIAMAH
Master course: Maiko TAKAGI
Tsuyoshi HORIKAWA
Kensuke WADA
Chie MASAKI
Saki HASHIMOTO
Takuya HYODO(1)OutlineMicrobiology covers several aspects of bacteriology, immunology and virology. Through the studies on various
microbes it is expected to understand host-parasite relationship and mechanisms of pathogenicity. Unlike thepast, microbiology has rapidly been drawn to the center of the biological stage.
Our laboratory mainly deals with viral oncogenesis and immunodeﬁciency in humans. Several projects arecarried out with particular emphasis on investigation into the mechanisms of viral replication and pathogenesis
induced by human retroviruses (HIV-1 and HTLV-I) and human herpes viruses. The purpose of many of thestudies being undertaken is to identify critical events and molecules responsible for the eﬃcient replication ofthese viruses, and in case of human retroviruses, those for transformation or destruction of normal lymphocytes.
Virological, immunological and molecular approaches are being applied for this purpose.

(2)

Research

The following studies have been extensively carried out in out laboratory with various biological and molecularbiological techniques:- Pathogenesis of HIV and HTLV (mutation, virulence,― 258 ―

Bio-Environmental Response

Bio-Environmental Response
apoptosis, polymorphism).
- Studies on signal transduction pathways targeted byviral proteins.
- Molecular cloning by genetic approaches of components
essential for virus replication in mammalian cells.

(3)

Education

We are engaged in the lectures and practices on the basic aspects of infections for the 2nd year medical students
and in the pre-clinical clerkship for the 4th year medical students. Students are also accepted in the ProjectSemester Program. Graduate course students carry out research on virology and oncology in the laboratory
and join seminars and progress meetings.
(4)

Lectures & Courses

Students can learn the structure, replication, function and genetics of micro-organisms as well as the hostpathogen interactions based on the front-line molecular and microbiological sciences.
(5)

Publications[Original Articles]
1. Ito H, Tanaka S, Akiyama Y, Shimada S, Adikrisna R, Matsumura S, Aihara A, Mitsunori Y, Ban D,
Ochiai T, Kudo A, Arii S, Yamaoka S, Tanabe M. Dominant Expression of DCLK1 in Human Pancreatic
Cancer Stem Cells Accelerates Tumor Invasion and Metastasis. PLoS ONE. 2016.01; 11(1); e0146564
2. Masaaki Sugimoto · Kenichi Kohashi · Momoe Itsumi · Masaki Shiota · Tatsuro Abe · Yuichi Yamada ·Kentaro Kuroiwa · Seiji Naito. Epithelial to Mesenchymal Transition in Clear Cell Renal Cell Carcinoma
with Rhabdoid Features J Natl Cancer Inst. 2016.02; 108(7);3. Momoe Itsumi · Masaki Shiota · Ario Takeuchi · Eiji Kashiwagi · Junichi Inokuchi · Katsunori Tatsugami· Shunichi Kajioka · Takeshi Uchiumi · Seiji Naito · Masatoshi Eto · Akira Yokomizo. Equol inhibits
prostate cancer growth via degradation of the androgen receptor by Skp2 2016.04;
4. Kwoﬁe KD, Tung NH, Suzuki-Ohashi M, Amoa-Bosompem M, Adegle R, Sakyiamah MM, Ayertey F,
Owusu KB, Tuﬀour I, Atchoglo P, Frempong KK, Anyan WK, Uto T, Morinaga O, Yamashita T, Aboagye
F, Appiah AA, Appiah-Opong R, Nyarko AK, Yamaguchi Y, Edoh D, Koram KA, Yamaoka S, Boakye DA,
Ohta N, Shoyama Y, Ayi I. . Antitrypanosomal Activities and Mechanisms of Action of Novel Tetracyclic
Iridoids from Morinda lucida Benth. Antimicrob Agents Chemother.. 2016.05; 60(6); 3283-3290
5. Masaki Shiota · Naohiro Fujimoto · Kenjiro Imada · Akira Yokomizo · Momoe Itsumi · Ario Takeuchi· Hidetoshi Kuruma · Junichi Inokuchi · Katsunori Tatsugami · Takeshi Uchiumi · Yoshinao Oda ·Seiji
Naito. Potential Role for YB-1 in Castration-Resistant Prostate Cancer and Resistance to Enzalutamide
Through the Androgen Receptor V7 Pathobiology.. 2016.05; 83(6); 277-286
6. Koﬁ D Kwoﬁe, Nguyen Huu Tung, Mitsuko Suzuki-Ohashi, Michael Amoa-Bosompem, Richard Adegle,
Maxwell M Sakyiamah, Frederick Ayertey, Koﬁ Baﬀour-Awuah Owusu, Isaac Tuﬀour, Philip Atchoglo,
Kwadwo K Frempong, William K Anyan, Takuhiro Uto, Osamu Morinaga, Taizo Yamashita, Frederic
Aboagye, Alfred A Appiah, Regina Appiah-Opong, Alexander K Nyarko, Yasuchika Yamaguchi, Dominic
Edoh, Kwadwo A Koram, Shoji Yamaoka, Daniel A Boakye, Nobuo Ohta, Yukihiro Shoyama, Irene Ayi.
Antitrypanosomal Activities and Mechanisms of Action of Novel Tetracyclic Iridoids from Morinda lucida
Benth. Antimicrob. Agents Chemother.. 2016.06; 60(6); 3283-3290
7. Shiota M, Fujimoto N, Itsumi M, Takeuchi A, Inokuchi J, Tatsugami K, Yokomizo A, Kajioka S, Uchiumi
T, Eto M.. Gene polymorphisms in antioxidant enzymes correlate with the eﬃcacy of androgen-deprivation
therapy for prostate cancer with implications of oxidative stress Ann Oncol.. 2016.12;― 259 ―

Bio-Environmental Response

Bio-Environmental Response

8. Ito H, Tanaka S, Akiyama Y, Shimada S, Adikrisna R, Matsumura S, Aihara A, Mitsunori Y, Ban D,
Ochiai T, Kudo A, Arii S, Yamaoka S, Tanabe M. . Dominant Expression of DCLK1 in Human Pancreatic
Cancer Stem Cells Accelerates Tumor Invasion and Metastasis. PLoS One. . 11(1); e0146564[Conference Activities & Talks]
1. Hiroaki Takeuchi. The timing and triggering of HIV-1 uncoating: the essential role of MELK for optimal
capsid core disassembly.. U.S-Japan Cooperative Medical Sciences Program (USJCMSP) AIDS Panel
Meeting 2016.01.13 Washington DC, USA
2. Momoe Itsumi, Masaki Shiota, Akira Yokomizo, Eiji Kashiwagi, Ario Takeuchi, Kenjiro Imada, Katsunori
Tatsugami, Junichi Inokuchi, Takeshi Uchiumi, Shunichi Kajioka, Seiji Naito, Masatoshi Etoh. Serotoninreceptor antagonist inhibits prostate cancer progression. The 75th Annual Meeting of the Japanese Cancer
Association 2016.10.06 Paciﬁco Yokohama
3. Takeshi Yoshida, Akiko Hamano, Hiroaki Takeuchi, Shoji Yamaoka. Identiﬁcation of a new host factor
inhibiting HIV-1 replication. 2016.11.24 Kagoshima
4. Hiroaki Takeuchi. Host factors regulated HIV-1 capsid disassembly. The 30th Annual Meeting of theJapanese Society for AIDS Research 2016.11.26 Kagoshima, JAPAN― 260 ―

Bio-Environmental
Bio-Environmental Response
Response

Immunotherapeutics

Professor: Mari KANNAGI
Associate Professor: Takao MASUDA
Assistant Professor: Atsuhiko HASEGAWA (Lecturer)
Assistant Professor: Yoshiko NAGANO
Visiting Researcher:: Sayaka ITO
Graduate Student: Yoko SATO, Satomi ANDO, Tatsuro TAKAHATA, Yuji MURAKAMI, Leila SAWADA, Riho KOG(1)OutlineOur research area is in between clinical and basic science, involving immunology, microbiology, and oncology.
Persistent viral infection causes various diseases by inducing immunodeﬁciency, malignancy, autoimmunity, andinﬂammation. Human immunodeﬁciency virus (HIV) causes acquired immunodeﬁciency syndrome (AIDS),
and Human T-cell leukemia virus type-I (HTLV-I) causes adult T-cell leukemia (ATL) and various chronic
inﬂammatory autoimmune-like diseases. To understand mechanisms of these diseases, investigation on hostimmunity is indispensable. Immune responses are usually protective but sometimes harmful for the host, andare important determinants for disease manifestation. The goal of our research is elucidation of the role ofhost immunity in the diseases in order to develop eﬀective immunotherapy. We also investigate intracellularmechanisms of viral replication to target direct molecules for therapy.
Research Subjects
1. Analysis of immunological risks for ATL development in HTLV-I-carriers.
2. Development of anti-tumor vaccine against ATL.
3. Immunological and molecular mechanism of HTLV-1-induced leukemogenesis.
4. Molecular mechanism of HIV replication especially related to HIV-1 integrase.
5. Experiments based on gene therapy to suppress HIV-1 replication.

(2)

Research① Development and clinical study of anti-ATL vaccine therapy with Tax peptide-pulsed autologous dendritic
cells.
Adult T-cell leukemia/lymphoma (ATL) is a human T-cell leukemia virus type-I (HTLV-I)-infected T-cell malignancy with poor prognosis. We developed a novel therapeutic vaccine designed to augment an HTLV-I Taxspeciﬁc cytotoxic T lymphocyte (CTL) response that has been implicated in anti-ATL eﬀects, and conducted apilot study to investigate its safety and eﬃcacy in collaboration of Tokyo Medical and Dental University, National Kyushu Cancer Center, and Kyushu University. The vaccine consists of autologous dendritic cells pulsedwith Tax peptides corresponding to the CTL epitopes. Two of three patients administered with the vaccine
achieved partial and complete remission without severe side eﬀects. The clinical outcomes of this pilot study
indicate that the Tax peptide-pulsed DC vaccine is a safe and promising immunotherapy for ATL（Suehiro, Y.,
Hasegawa, A., et al. Brit J Haematol. 169: 356-367, 2015. doi: 10.1111/bjh.13302）.
② Involvement of innate immune response in HTLV-1 pathogenesis.
The constitutive activation of NF κ B plays an important role in leukemogenesis of adult T-cell leukemia/lymphoma(ATL) caused by human T-cell leukemia virus type-1 (HTLV-1). Although HTLV-1 Tax is known to activate― 261 ―

Bio-Environmental Response
Response
Bio-Environmental

NF κ B, ATL cells exhibit NF κ B activities even in the absence of Tax expression, the mechanism of which
has been a long-puzzling question. We demonstrate that both double-stranded RNA-dependent protein kinase(PKR) and anti-sense HTLV-1 transcripts are involved in the constitutive NF κ B activation in Tax-negative
ATL cells. Our ﬁndings elucidate a novel Tax-independent mechanism of NF κ B activation underlying HTLV-1
leukemogenesis in which host antiviral responses are involved (Kinpara, S., et al. Leukemia, 29:1425-1444, 2015.
doi: 10.1038/leu.2015.1).

(3)

Education① For under graduate students of the medical school, we participate in education of basic immunology I, andII, the project semester, and the preclinical clarkship.
② Graduate students are trained for basic skills in the ﬁeld of immunology and virology to handle biohazard
materials. We provide the opportunity to research for mechanisms of the retro-virus-mediated diseases anddevelopment of immunological therapeutics. All the stuﬀs and students participate in maintenance of thelaboratory and periodical seminars to discuss about their own studies and keep up with the latest knowledge
and information in the area.

(4)

Lectures & Courses

We always think of the clinical signiﬁcance of the results of basic research. We try to ﬁnd an eﬀective therapy
by approaching from basic research to understand the disease mechanisms and solve the problem. The diseasemechanisms that we study include leukemogenesis, inﬂammation, immunosuppression, and autoimmunity inpersistent virus infection. Through these studies, we contribute to clinical therapies as well as medical sciences.
(5)

Clinical Services & Other Works

We developed an anti-ATL immunotherapy (Tax peptide-pulsed dendritic cell vaccine), which is under clinicalstudies in collaboration with National Kyushu Cancer Center and Kyushu University. We evaluate anti-tumor
and anti-virus T-cell responses in HTLV-1-infected patients with or without various therapies including theimmunotherapy and hematopoietic stem cell transplantation, in response to requests from clinical doctors.
(6)

Publications[Original Articles]
1. Takahata, T., Takeda, E.,Tokunaga, K., Yokoyama, M., Huang, Y-L., Hasegawa, A., Shioda, T., Sato
H, Kannagi, M., Masuda T. Critical Contribution of Tyr15 in the HIV-1 Integrase (IN) in Facilitating
IN Assembly and Nonenzymatic Function through the IN Precursor Form with Reverse
Transcriptase.
J. Virol.. 2016; 90; 4563-4578
2. Masako Nomaguchi, Naoya Doi, Yosuke Sakai, Hirotaka Ode, Yasumasa Iwatani, Takamasa Ueno, Yui
Matsumoto, Yasuyuki Miyazaki, Takao Masuda, Akio Adachi. Natural Single-Nucleotide Variations in
the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression
Levels. J. Virol.. 2016.05; 90(9); 4563-4578― 262 ―

Bio-Environmental
Bio-Environmental Response
Response

Cellular and Environmental Biology

Associate Professor Masayuki HARA
(1)

ResearchResearch Subjects
1) Reaction mechanisms of cellular protection systems against environmental oxidation stresses.
2) Modifying mechanisms in higher order structure of chromatin in cellular diﬀerentiation.
3) Shifting mechanisms in proteome proﬁles of cell organelle between pre and post conditions in environment,
cell diﬀerentiation, disease, or drug exposure.

(2)

Education

Living organisms were inﬂuenced their life by environment and adapted themselves to it, however, they formed
environment and aﬀected it. In other words, the species that cannot ﬁt the changing environment were fallen andreplaced by the new species which could adapt itself to. The organisms are as a part of the global environment,
so it is thought that the individual structure and working of them are necessary environmental measures for theirsurvival. It may be said that it is excessive suddenness of the change that human activity is environmentallyimpacted now.
Main objective of cellular and environmental biology in the graduate course is to provide students opportunity
to study the reaction and adaptation of the organisms for the environmental change at cellular level, to consider
hazardous property, toxicity, or physiological activity of environmental (or man-made) factor, and to mention
the biotechnical action to the environmental problems.

(3)

Publications[Original Articles]
1. Masayuki Hara, Kiyoshi Nomura. A Model Calculation and its Interpretation of the Rradiation Eﬀects
Based on the Ionization of Intracellular Water Molecules. Japanese Journal of Radiation Safety Management. 2016.11; 15(2); 164-172― 263 ―

Bio-Environmental Response

Advanced Molecular Medicine

Biodefense Research

Professor Toshiaki Ohteki
Junior Associate Professor (to Dec. 14) Nobuyuki Onai
Adjunct Lecturer (from Dec.15) Nobuyuki Onai
Adjunct Lecturer (JST PREST) Taku Sato
Assistant Professor Yusuke Nakanishi
Junior Assistant Professor Junpei Asano
Junior Assistant Professor Mihoko Kajita
Research Fellow (SONY) Tomohiko Nakamura
Research Fellow Shunsuke Kawamura
Graduate Student Minako Inazawa
Graduate Student Kana Minamide
Graduate Student Shuhei Imamura
Research Technician Shoko Kuroda
Research Technician Kisho Shiseki
Research Technician Rumiko Nakamura
Secretarial Assistant Hisako Kamioka(1)

OutlineOur research projects focus on understanding the dynamic maintenance and transﬁguration of homeostasis
in the living body. Our goal is to deﬁne the homeostasis mechanism under conditions of health and disease.
To accomplish this goal, we are trying to clarify the molecular basis of induction and failure of homeostasis
by focusing on immune cells in particular mononuclear phagocytes (dendritic cells and macrophages), tissue
stem cells, and their functional interplay in the immunological and non-immunological organs, such as skin andintestine. On the basis of our ﬁndings, we will further pursue our research in the hope of developing new rational
therapies for prevention and treatment of disease.
(2)

Research

1. Research on mononuclear phagocytes
1) Discovery of a novel source of mononuclear phagocytes
In 1968, Drs. Ralph van Fruth and Zanvil A. Cohn proposed a concept of mononuclear phagocytes that include
monocytes and macrophages. In 1973, Dr. Ralph Steinman discovered dendritic cells (DCs), thereby redeﬁning
the mononuclear phagocytes as a population consisting of monocytes, macrophages and also DCs. It has been
recently continuing epoch-making discoveries in the ﬁeld of mononuclear phagocytes and their functions are now
beyond classical Immunology and rather extend to broad life phenomenon, e.g. tissue development/regeneration,wound-healing, and establishment of various inﬂammatory diseases.
DCs consist of conventional DCs (cDCs) and plasmacytoid DCs (pDCs), both of which play critical regulatory
roles in the immune system. cDCs exhibit prominent antigen-presenting ability, whereas pDCs are characterized
by their capacity to produce large amounts of type I interferons (IFNs). We have discovered the DC progenitors
in the mouse bone marrow, and named common DC progenitors (CDPs) (Immunity 2013; Nat Immunol 2007).
Interestingly, CDPs are divided into 2 subpopulations. One is M-CSF receptor (R)+ CDPs mainly producing
cDCs, and the other M-CSFR-CDPs producing a large number of pDCs. In addition to CDPs, common
monocyte/macrophage progenitors, cMoP, identiﬁed in the mouse bone marrow and spleen by other group in― 264 ―

Bio-Environmental Response

Advanced Molecular Medicine

2013.
Based on these achievements in mouse, we have been trying to identify human progenitors of mononuclear
phagocytes, and most recently succeeded to identify human cMoP (in revision). Human cMoP gives rise to
only monocytes but not other hematopoietic cells including DCs. Given that monocytes and monocyte-derived
macrophages cause a variety of inﬂammatory disorders, including metabolic syndromes and tumor development,
our studies shed light on possible therapeutic applications for infectious diseases, cancers and autoimmune
diseases.
2) Roles of mononuclear phagocytes in inﬂammatory bowel disease
Breakdown of the intestinal epithelial layer s barrier function results in the inﬂow of commensal ﬂora
and improper immune responses against the commensal ﬂora, leading to inﬂammatory bowel disease (IBD)
development. Using a mouse dextran sodium sulfate (DSS)–induced colitis model, we showed that commensal
Gram-positive bacteria trigger the mobilization of inﬂammatory monocytes and macrophages into the colon(Mucosal Immunol 2015). TNF-α, a representative cytokine that aggravates colitis and a promising therapeutictarget, was predominantly produced by monocytes/macrophages. Among macrophage subpopulations, Ly6c+
macrophages were a major colitogenic subset producing TNF-α. In addition, IFN-γ–Stat1 pathway was
required for histone acetylation at the promoter regions of the Tnf loci in macrophages, indicating that IFN-γ–
dependent epigenetic regulation instructs the development of colitogenic macrophages. Our study may provide
new therapeutic targets, e.g. inhibition of acetyl transferase in macrophage, for treating IBD and colon cancer(in revision).
2. Research on tissue stem cells
1) Understanding of tissue homeostasis and its breakdown on the basis of immune cell-tissue stem cell interplay
We found that type I IFNs induce proliferation and exhaustion in hematopoietic stem cells (HSCs), andthat interferon regulatory factor-2 (IRF2), a transcriptional suppressor of type I IFN signaling, preserves theself-renewal and multi-lineage diﬀerentiation capacity of HSCs (Nat Med 2009). Based on this ﬁnding, weshow that type I IFN preconditioning, without irradiation or DNA alkylating agents, signiﬁcantly enhanced
the HSC engraftment eﬃciency in wild type (WT) recipient mice (Blood 2013). Based on these achievements,
we have further found that physiological levels of type I IFN signaling also aﬀect other tissue stem cells, e.g.
intestinal stem cells (ISCs) and hair follicle stem cells (HFSCs). Elucidation of detailed mechanisms is currently
in progress.
3. Collaborative research with other institutes
In collaboration with RIKEN, Institute of Physical and Chemical Research, we performed microbiota analysis
by 16S rRNA sequencing, and found that there is no signiﬁcant change in the feces of mice with excess IFN
signals speciﬁcally in intestinal epithelial cells. As the mice showed defective regeneration capacity of ISCs, weconcluded that it is unlikely due to the altered commensal composition (manuscript in preparation).

(3)

Education

Immunology lectures in Faculty of Medicine, Masters Degree, and Doctoral Programs, Graduate School Seminarin other universities as a adjunct lecturer, and educational and research guidance for individual graduate
students.
(4)

Publications[Original Articles]
1. Nobuyuki Onai, Toshiaki Ohteki. Isolation of Dendritic Cell Progenitor and Bone Marrow Progenitor
Cells from Mouse. Methods Mol. Biol.. 2016.01; 1423; 53-59
2. Jiajia Liu, Yong-Mei Guo, Nobuyuki Onai, Hideaki Ohyagi, Makoto Hirokawa, Naoto Takahashi, Hiroyuki Tagawa, Kumi Ubukawa, Isuzu Kobayashi, Hiroyuki Tezuka, Yoshihiro Minamiya, Toshiaki Ohteki,
Kenichi Sawada. Cytosine-Phosphorothionate-Guanine Oligodeoxynucleotides Exacerbates Hemophagocytosis by Inducing Tumor Necrosis Factor-Alpha Production in Mice after Bone Marrow Transplantation.
Biol. Blood Marrow Transplant.. 2016.04; 22(4); 627-636― 265 ―

Bio-Environmental Response

Advanced Molecular Medicine

3. Takayuki Yokoi, Kentarou Yokoi, Kazumasa Akiyama, Takashi Higuchi, Yohta Shimada, Hiroshi Kobayashi,
Taku Sato, Toshiaki Ohteki, Makoto Otsu, Hiromitsu Nakauchi, Hiroyuki Ida, Toya Ohashi. Nonmyeloablative preconditioning with ACK2 (anti-c-kit antibody) is eﬃcient in bone marrow transplantation
for murine models of mucopolysaccharidosis type II. Mol. Genet. Metab.. 2016.11; 119(3); 232-238[Awards & Honors]
1. Young Investigator’s Aword (Shunsuke Kawamura), The 24th International Symposium on Molecular Cell
Biology of Macrophages , 2016.06― 266 ―

Bio-Environmental
Bio-Environmental Response
Response

Pathological Cell Biology

Professor：Shigeomi SHIMIZU
Associate Professor：Norio SHIMIZU
Junior Associate Professor：Satoko ARAKAWA
Project Associate Professor：Masatsune TSUJIOKA, Satoshi TORIIAssistant Professor：Shinya HONDA
Project Assistant Professor：Hirofumi YAMAGUCHI,
Michiko MUROHASHI, Nobuhiro FUJIKAKE, Hajime SAKURAI
Tokunin Assistant Professor： Minkyon SHIN, Mariko SUNADA
Postdoctoral fellow (PD1)：Go Yoshida
Secretary：Hitomi Fukabori, Setsu TAMAI, Kayo HATTANDA
Research Assistant：Ikuyo YOSHINO, Kyoko TSUJIMURA, Ikuko NAKANOMYO, Naomi KOJIMA,
Hikari SHIMADA, Yuta YUNOMAE, Yumi IWASAKI, Hiromi KOJIMA
Graduate Student：Yuna SUGIMOTO, Toyokazu SEKI, Saori NOGUCHI, Yuta GOTO,
Miyuki NAKAI, Hatuki ENDO, Hajime MOCHIDUKI, Ryo OKUNO(1)Outline1) Analysis of apoptosis mechanism
2) Analysis of non-apoptotic cell death (autophagic cell death)3) Physiological and pathological roles of cell death in mammals
4) Analysis of alternative macroautophagy mechanism5) Physiological and pathological roles of autophagy in mammals
6) Development of novel EBV infection animal models using the hNOG mice7) Development of an exhaustive pathogenic microbe screening system(2)

Research

Main objective in the graduate course is to provide students opportunity to study the molecular mechanisms ofcell death and autophagy, the cell death-related diseases, the physiological and pathological roles of autophagy,
and the development mechanism of Epstein-Barr virus (EBV) infection, the employment of immunodeﬁciency
animals for the creation of virus research models and development of an exhaustive pathogenic microbial screening system.
(3)

Education

Main objective in the graduate course is to provide students opportunity to study the molecular mechanisms ofcell death and autophagy, the cell death-related diseases, the physiological and pathological roles of autophagy,
and the development mechanism of Epstein-Barr virus (EBV) infection, the employment of immunodeﬁciency
animals for the creation of virus research models and development of an exhaustive pathogenic microbial screening system.

― 267 ―

Bio-Environmental Response
Response
Bio-Environmental(4)

Publications[Original Articles]
1. Arai A, Sakashita C, Hirose C, Imadome K, Yamamoto M, Jinta M, Fujiwara S, Tomita M, Shimizu
N, Morio T, and Miura O.. Hematopoietic stem cell transplantation for adults with EBV-positive Tor NK-cell lymphoproliferative disorders: eﬃcacy and predictive markers Bone Marrow Transplantation.
2016;
2. Uchiyama H, Uehara K, Nagashima T, Nakata A, Sato K, Mihara Y, Komatsu K, Takanari J, Shimizu
S, Wakame K.. Global Liver Gene Expression Analysisi on a Murine Metabolic Syndrome Model Treatedby Low-molecular-weight Lychee Fruit Polyphenol. Anticancer Research.. 2016; 36(7); 3705-3713
3. Yoshida T, Tsujioka M, Honda S, Tanaka M, Shimizu S.. Autophagy suppresses cell migration by degrading GEF-H1, a RhoA GEF. OncoTaget.. 2016; 7(23); 34420-34429
4. Nasu Y, Benke A, Arakawa S, Yoshida GJ, Kawamura G, Manley S, Shimizu S, Ozawa T. In Situ Characterization of Bak Clusters Responsible for Cell Death Using Single Molecule Localization Microscopy.
Scientiﬁc Reports.. 2016; 13(6); 27505
5. Yamaguchi H, Arakawa S, Kanaseki T, Miyatsuka T, Fujitani Y, Watada H, Tsujimoto Y, Shimizu S..
Golgi membrane-associated degradation pathway in yeast and mammals. EMBO J.. 2016; 35(18); 19912007
6. Konishi A, Izumi T, Shimizu S.. TRF2 Protein Interacts with Core Histones to Stabilize Chromosome
Ends. J.Biol.Chem.. 2016; 291(39); 20798-20810
7. Torii S, Yoshida T, Arakawa S, Honda S, Nakanishi A, Shimizu S.. Identiﬁcation of PPM1D as an essential
Ulk1 phosphatase for genotoxic stress-induced autophagy. EMBO R.. 2016; 17(11); 1552-1564
8. Watanabe Y, Honda S, Konishi A, Arakawa S, Murohashi M, Yamaguchi H, Torii S, Tanabe M, Tanaka
S, Warabi E, Shimizu S.. Autophagy controls centrosome number by degrading Cep63. Nature Commun..
2016; 7; 13508
9. Natsuko Inazawa, Tsukasa Hori, Masaki Yamamoto, Naoki Hatakeyama, Yuko Yoto, Masanori Nojima,
Hiroshi Yasui, Nobuhiro Suzuki, Norio Shimizu, Hiroyuki Tsutsumi. HHV-6 encephalitis may complicate
the early phase after allogeneic hematopoietic stem cell transplantation: Detection by qualitative multiplex
PCR and subsequent quantitative real-time PCR. J. Med. Virol.. 2016.02; 88(2); 319-323
10. Natsuko Inazawa, Tsukasa Hori, Masanori Nojima, Makoto Saito, Keita Igarashi, Masaki Yamamoto,
Norio Shimizu, Yoko Yuto, Hiroyuki Tsutsumi. Virus reactivations after autologous hematopoietic stem
cell transplantation detected by multiplex PCR assay. J. Med. Virol.. 2016.07;
11. Shotaro Ando, Jun-Ichi Kawada, Takahiro Watanabe, Michio Suzuki, Yoshitaka Sato, Yuka Torii, Masato
Asai, Fumi Goshima, Takayuki Murata, Norio Shimizu, Yoshinori Ito, Hiroshi Kimura. Tofacitinib induces
G1 cell-cycle arrest and inhibits tumor growth in Epstein-Barr virus-associated T and natural killer cell
lymphoma cells. Oncotarget. 2016.11; 7(47); 76793-76805
12. Ng SB, Ohshima K, Selvarajan V, Huang G, Choo SN, Miyoshi H, Shimizu N, Reghunathan R, Chua HC,
Yeoh AE, Quah TC, Koh LP, Tan PL, Chng WJ. . Epstein-Barr virus-associated T/natural killer-cell
lymphoproliferative disorder in children and young adults has similar molecular signature to extranodal
nasal natural killer/T-cell lymphoma but shows distinctive stem cell-like phenotype. Leuk Lymphoma..
56; 2408-2415
13. Wu T, Wang S, Wu J, Lin Z, Sui X, Xu X, Shimizu N, Chen B, Wang X.. Icaritin induces lytic cytotoxicity
in extranodal NK/T-cell lymphoma. J Exp Clin Cancer Res. . 34; 17
14. Kozaki T, Komano J, Kanbayashi D, Takahama M, Misawa T, Satoh T, Takeuchi O, Kawai T, Shimizu
S,Matsuura Y, Akira S, Saitoh T.. Mitochondrial damage elicits a TCDD-inducible poly(ADP-ribose)polymerase-mediated antiviral response. PNAS..
[Books etc]
1. Shimizu S. Autophagic Cell Death and Cancer Chemotherapeutics. . Springer,― 268 ―

Bio-Environmental
Bio-Environmental Response
Response[Misc]
1. Arakawa S, Honda S, Torii S, Tsujioka M, Shimizu S.. Monitoring of Atg5-independent Mitophagy.
Methods in Molecular Biology,"Mitophagy".
[Conference Activities & Talks]
1. Shimizu S. Identiﬁcation of natural puroduct against fatty liver disease based on the induction of alternative autophagy. ICNIM2016 2016.07.15 Sapporo
2. Haruna Shibayama, Ken-ichi Imadome, Chizuko Sakashita, Ken Watanabe, Norio Shimizu, Takatoshi
Koyama, Shigeyoshi Fujiwara, Osamu Miura, Ayako Arai. In vitro and in vivo eﬀects of proteasome
inhibitor bortezomib on Epstein-Barr virus-positive T- or NK-cell lymphoproliferative diseases. 17th
International Symposium on Epstein-Barr virus (EBV) and Associated Diseases 2016.08.10 Zurich
3. Shimizu S. Alterntive Autophagy is Essential for Neuronal Cell Maintenance. Brain Protein Aiging and
Dementia Contorol International Workshop 2016.09.09 Nagoya
4. Haruna Shibayama, Ken-ichi Imadome, Chizuko Sakashita, Ken Watanabe, Fuyuko Kawano, Norio Shimizu,
Takatoshi Koyama, Shigeyoshi Fujiwara, Osamu Miura, Ayako Arai. In vitro and in vivo eﬀects of bortezomib on EBV-T/NK-LPDs. The 78th Annual Meeting of the Japanese Society of Hematology 2016.10.14
Yokohama
5. Shimizu S. Mitophagy and alternative autophagy. The 13th Conference of Asian Society for Mitochondrial
Reseach and Medicine 2016.10.31 Shinagawa― 269 ―

Bio-Environmental Response

Bio-Environmental Response

Pediatrics and Developmental Biology

Professor: Tomohiro MORIO
Associate Professor: Hirokazu KANEGANE
Assistant Professor: Kenichi KASHIMADA, Atsuko TAKI
Project Assistant Professor:
Eriko TANAKA, Masakatsu YANAGIMACHI, Chikako MORIOKA, Noriko MITSUIKI, Yoshichika MAEDA,
Shinpei BABA, Kengo MORIYAMA, Norimasa TADA, Ryo AOKI, Keisuke OKAMOTO
Graduate Students:
Toshiaki ONO, Tomohiro UDAGAWA, Keisuke TANAKA, Tomonori SUZUKI, Risa NOMURA,
Yusuke KAJIKAWA, Atsumi HOSOKAWA-TSUJI, Motoi YAMASHITA, Tsubasa OKANO, Ayako KASHIMADA,
Chika KOBAYASHI, Yuichi MIYAGAWA, Akito SUTANI, Yohei YAMAGUCHI, Shintaro ONO,
Fumika TOKUNAGA, Eri KUMAKI, Miko SHIGENO, Lin LIN, Ouwen YOU, Rina NISHII
Department of Pediatrics, Neonatal and Maternal Medicine
Professor: Shozaburo DOI
Associate Professor: Kohsuke IMAI, Masatoshi TAKAGI(1)

OutlineOur department delivers the most advanced care available today to infants and children. The specialties of ourdepartment cover most pediatric diseases, including hematology-oncology, immunology, cardiology, neurology,
endocrinology, nephrology, neonatology, allergy and rheumatology. On the other hand, our scientiﬁc and academic activities encompass a wide spectrum of basic and clinical areas and all levels of biologic organization,from the molecule to international populations. The goal of our research is to improve the health of children.
We focus on innovation and the development of new strategies for clarifying pathogenesis, diagnostic tests, andtherapeutic interventions.
(2)

Research

Our research covers many specialties of pediatric diseases, and the researches are based on from bench to bedside. Our current main projects are
1. Identiﬁcation of responsible genes for primary immunodeﬁciency (PID) development.
2. Development of therapeuti approach for PID
3. Research in autoimmune lymphoproliferative syndrome (ALPS) and RAS-associated ALPS like syndrome
4. Quality assessment of iPS cells for clinical application
5. Regulation of granulocyte activation and apoptosis
6. Development of innovative techniques for immune therapy after hematopoietic stem cell transplantation.
7. Elucidating the pathogenesis of glomerulosclerosis formation and the mechanisms in podocyte.
8. Research of infectivity in patients with refractory nephrotic syndrome
9. Revealing the mechanisms of immunological relevant to pediatric idiopathic nephrotic syndrome.
10. Mechanisms of idiopathic pulmonary arterial hypertension
11. Guideline for cardiomyopathy in children
12. Social background in pediatric heart transplantation― 270 ―

Bio-Environmental Response

Bio-Environmental Response

13. Clinical background of Eisenmenger syndrome in Japan
14. Lung injury induced by cytokines/monocytes/granulocytes
15. Pathogenesis of periventricular leukomalacia and bronchopulmonary dysplasia
16. Elucidating the molecular mechanisms of gonadal development
17. Molecular pathology of congenital adrenal diseases and disorder of sex development
18. Molecular pathology of diabetes mellitus caused by mutations of the insulin receptor
19. ATM dependent cellular diﬀerentiation
20. Neural diseases based on defective DNA damage response
21. Investigation of molecule marker determine the prognosis of infant leukemia
22. Development of therapeutic strategy targeting homologous recombination repair
23. Genetic background of leukemia development
We are collaborating with Medical Research Institute at TMDU, Tokyo University, Institute of Medical Science,
Hiroshima University, Istitute Nazionale Tumori (Dr. D. Delia), University of Queensland (Prof. PeterKoopman), Erasmus University (Prof. Jacques van Dongen), Yonsei University (Profs. H. Kim, and SK Lee),Sony Life Science Laboratories, National Institute for Longevity Sciences, National Research Institute for Child
Health and Development, RIKEN Center for Integrative Medical Science, Kazusa DNA Research Institute,Tokyo Metropolitan Institute for Medical Science, Juntendo University, and many other laboratories.
● Hematology/Oncology/Immunology Group (Basic Research)
Our research focuses on the dissection of molecular basis of immune regulation, cancer development, DNAdamage response. Our aim in research focusing on elucidation of molecular pathogenesis and development oftherapeutic approach for pediatric catastrophic disease, especially in immunological disorder. Our researcharea is also located in the ﬁeld of tumor development, DNA damage response and primary immunodeﬁciencies.
Some of these research is tightly linked each other. The directions; from bench to clinic and clinic to bench
is favorably utilized for research style. Nationwide survey for common variable immunodeﬁciency (CVID) is
executing under the leader
ship of Dr. Morio. Through the analysis of patient samples using next generation sequencing technology, we
have identiﬁed several rare disease causing gene mutations from a CVID patients, and pursuing characterization. Research focusing on X linked lymphoproliferative disease has been conducted by Dr. Kanegane. iPS cell
directed innovative drug discovery has been performed by Dr. Takagi collaborated with Dr. Ohtsu in Tokyo
University. In terms with ataxia telangiectasia, research group was organized by Dr. Takagi to improve patient
outcome. Cellular diﬀerentiation has been investigated using patient derived iPS cells. To maintain quality oflife in PID, web based interaction between patient side and hospital side has been established under leadershipby Dr. Imai.
In the clinical setting, viral infection is one of a critical factor that aﬀect the outcome of hematopoietic stem
cell transplantation. To overcome this disadvantage, we have been starting the generation of multi virus speciﬁccytotoxic T cell. This year our group revealed how chromosomal integrated HHV6 is activated in severe combined immunodeﬁciency patient. This ﬁndings is notable discovery in ﬁeld of infection and immunodeﬁciency.
In regenerative medicine ﬁeld, Dr. Morio organized research group to evaluate the quality of clinical use iPS
cell. Comprehensive microbe monitoring system has been developing collaborate with Dr. Shimizu at TMDU
Medical research institute, and non-invasive genomic alteration detection system has been developing collaborate
with Dr. Inazawa at TMDU Medical research institute and Dr. Ohara at Kazusa genome institute.
In the oncology ﬁeld, we have focusing on that how DNA damage response prevent oncogenic transformation.
In addition, development of therapeutics targeting DNA damage and repair pathway is conducting. Comprehensive genome research for leukemia has been conducted. These research will be lead to identiﬁcation of novel
therapeutic approach for pediatric leukemia. Clinical trial based on these discovery is currently under consideration.
● Cardiology GroupWe performed both the basic and clinical studies.
As for the basic studies, we have consecutively focused on the theme inﬂammation and pulmonary hypertension(PH) and have engaged in the basic research to clarify the mechanisms of PH and development of treatment.
However, we moved to focus on pulmonary hypertension (PH) induced right ventricular (RV) remodeling which
is one of the important factors related to its prognosis. We became to be interested in Pannexin (PANX) which
can play a role as heart mechanosensor. PANX is a protein which exists in cell membrane and belongs to be
the gap junction channel family. Dr. Furukawa is Professor of department of Bio-informational Pharmacology― 271 ―

Bio-Environmental Response

Bio-Environmental Response

and clariﬁed that PANX expressed on the left ventricle was associated with TAC-induced left ventricular (LV)
hypertrophy by using PANX K/O mice. Our research aim is to clarify that PANX plays an important rolefor PH-induced RV hypertrophy as heart mechanosensor as well as TAC-induced LV hypertrophy. It may bepossible to improve the prognosis in PH patients by regulation of PANX which is expressed on the RV.
AS for the clinical studies, we were engaged in three multi-center-associated clinical studies. Two of them
Guideline for cardiomyopathy in children and Social background in pediatric heart transplantation were
supported by Health and Labor Sciences Research Grant, and one of them Clinical backgrounds of Eisenmenger
syndrome in Japan was supported by The Japanese Society of Pediatric Cardiology and Cardiac Surgery.
● Neurology Group
Our research is focused on elucidating the pathogenesis of neurological disorders, such as neurodegenerative andneuroinﬂammatry diseases, based on molecular approach.
Ataxia-telangiectasia (AT) is one of the major neurodegenerative diseases caused by defective DNA damage
responses. We established iPS cell lines from AT patients and investigating the neuropathological mechanism
and trying to identify a novel therapeutic approach. Further, we are exploring to discover unidentiﬁed genetic
diseases with DNA repair-deﬁciency disorders using next-generation sequencing technology.
Marinesco-Sjögren syndrome, a disease associated with ER(Endoplasmic Reticulum) stress, is caused by abnormal unfolded protein response, and we aim to identify the pathological mechanisms at the cellular level. We
expect our research would provide therapeutic approach to the disease.
In collaborating with other research institutes, we have identiﬁed the adverse eﬀect of BZDs on a mouse modelof inﬂammation-induced status epilepticus. We showed that MDZ therapy signiﬁcantly increased the risk forneurological sequelae in immature mice, including hyperactivity (collaborative project with Haruo Okado, Tokyo
Metropolitan Institute of Medical Science).
Elucidating immunological roles of the purinergic receptor signal for microglial inﬂammatory reaction is another target of our research. We have established P2RY12 KO microglial cell line that enabled us to investigate
purinergic receptor signals on inﬂammatory cytokine production from microglia (collaborative project with Hiroshi Sakuma, Tokyo Metropolitan Institute of Medical Science).
Clinical and epidemiological study is another priority ﬁeld. We have been conducting a clinical research toevaluate the eﬃcacy and safety of very-low-dose betamethasone therapy in ataxia-telangiectasia. In multiinstitutional study, conducted by Shinichi Hirose, Fukuoka University, we have provided the genetic tests forthe patients with epilepsy and febrile-seizures. We have also contributed to a nationwide survey of rare intractable epilepsy syndrome registry project, operated by Yushi Inoue, NHO Shizuoka Institute of Epilepsy and
Neurological Disorders.
● Endocrinology Group
Currently, our research is focused on elucidating the molecular mechanisms of congenital diseases of endocrineorgans, especially adrenal glands and gonads. We are looking at developing the radical treatment systems forthe congenital endocrine diseases by using regenerative medicine as a ﬁnal target.
Our ongoing projects are bellows#1: Molecular mechanisms of gonadal development
We are trying to elucidate molecular mechanisms of gonadal development, especially, focusing on elucidating
epigenetic network of gonadal development and gonadal cell diﬀerentiation. Our projects also include identifying
the precise function of transcription factors, such as Sf1, Foxl2 and Sox9.
In collaborating with M. Kanai (Department of Experimental Animal Model for Human Disease), Shuji Takada(Department of Systems BioMedicine, National Center for Child Health and Development), P. Koopman (IMB.
The university of Queensland, Brisbane, Australia), we use in vitro and in vivo approach.
#2: Molecular pathology in congenital adrenal hyperplasia.
Congenital adrenal hyperplasia is one of disorders of sex development (DSDs), and now we are treating morethan 40 patients.
Including the relationships between genotype and phenotype, the molecular mechanisms of the disease have notbeen clariﬁed yet. Including functional analyses of novel mutations of the steroidogenic enzymes, we are trying
to elucidate the pathological mechanisms of the disease.
#3: Elucidating the mechanisms of congenital disorder of endocrine organs
Identifying the molecular mechanisms of congenital disorder of endocrine organs is also our important target.
Understanding the molecular pathology of the diseases will lead to develop innovative treatment of the diseases.
Current ongoing projects will be integrated systematicall, and will be applicable to develop innovative treatmentof congenital endocrine disorder, including approaches using regenerative medicine.

― 272 ―

Bio-Environmental Response

Bio-Environmental Response● Nephrology GroupWe are conducting researches to elucidate the mechanisms glomerulosclerosis and nephrotic syndrome: Elucidating the pathogenesis of glomerulosclerosis formation and the mechanisms in podocyte, research of infectivity
in patients with refractory nephrotic syndrome, and revealing the mechanisms of immunological relevant topediatric idiopathic nephrotic syndrome.
We are collaborating research with Division of Nephrology, Department of Internal Medicine, Juntendo University.
We participate in multi-institutional joint research of refractory nephrotic syndrome operated by Japanese Study
Group of Kidney Disease in Children.
● Neonatology group
We are investigating a novel therapy with umbilical cord blood derived mesenchymal stem cells for treating
periventricular leukomalacia using intrauterine infection model in cooperation with division of Cellular Physiological Chemistry and Nanomedicine (DNP) in TMDU.
● Allergy GroupOne of our main project goals is to elucidate the immunological mechanisms of food allergy such as that against
milk and eggs. Clinical and epidemiological study on food allergy is another major ﬁeld in our study. We
are conducting clinical studies of speciﬁc oral tolerance induction in food allergy in which the oﬀending foodis administered orally in order to achieve tolerance. Changing dietary habits in our country has led to an increases in allergies induced by uncommon food allergens, therefore we are analyzing the frequency and severityof immediate-type reactions induced by foods other than major food allergens such as hen eggs, cow s milk
and wheat in early childhood.
In collaboration with the Japanese Society of Pediatric Allergy and Clinical Immunology, we are conducting several clinical studies to reﬁne pharmacologic therapy listed in the Japanese pediatric guideline for the treatment
and management of asthma.

(3)

Education

The new curriculum of education which was started on April, 2011 ﬁnally covered all graders from the 1st to
6th of the Medical Science in the School of Medicine. In fact, the 6th graders were ﬁrst educated under the new
curriculum.
The education of the 19 basic medical sciences was ﬁnished in the 2nd graders under the new curriculum, so
the education of the clinical medicine was started from the ﬁrst of the 3rd graders. The 3rd graders and theﬁrst two months of the 4th graders were proposed systematic lectures, which was called Block Lecture andperformed together with Pediatrics, Obstetrics and Gynecology as Reproduction and Development block.
The Block Lecture was composed of 25 lectures including introduction, laboratory examination, metabolism,
emergency, nutrition, cardiology, gastroenterology, infection, allergy, endocrinology(2), development, hereditarydisorder, neonatology(2), neurology(2), pediatric dentist, immunology, collagen disease, child health, hematology
and oncology, nephrology, home healthcare and child abuse, four topics including fetus medicine, transitional
medicine, advanced medicine and pediatric psychiatry, three cases including congenital heart disease, two hybridwith delivery and neonatology, and one and a half team based learning (TBL).
Moreover, we also took charge of several lectures in another blocks, for example ﬁve lectures and one case related
to pediatric cardiology in Cardiology block, three lectures and one case related to pediatric oncology inOncology and Malignancy block, one lecture related to pediatric pulmonology in Pulmonology block,and three lectures related to pediatric infection in Infection and Clinics block. Total 47 systematic lectures
were almost produced by the staﬀs of Department of Pediatrics and Developmental Biology, and Departmentof Pediatrics, Perinatal and Maternal Medicine except ﬁve lectures by two another department staﬀs and three
part-time lecturers.
Opportunity of training in scientiﬁc research, so-called project semester, was provided for the 4th graders during
June to November. Three student was engaged in the pediatric research in 2016. Then the 5th graders weredivided into the small groups, and started and continued for three months to learn the introduction of ClinicalClerkship, so-called Pre-clerkship. It was also performed together with Pediatrics, Obstetrics and Gynecology
as Reproduction and Development block. The small group education chieﬂy for diﬀerential diagnosis was
performed and divided into seven sessions, which was load test and mass screening in endocrine disorders,
neurological evaluation for development, oncology, and immunology, renal function and ﬂuid therapy, neonatal
evaluation and diseases, congenital heart diseases, infection. The clinical reasoning, that is how to proceed thediﬀerential diagnosis, was very important for the clinicians and adopted in PCC since 2014.

― 273 ―

Bio-Environmental Response

Bio-Environmental Response

After the OSCE and CBT was ﬁnished in the end of the 4th graders, clinical clerkship (CC) was started
at the beginning of the 5th graders. One month practice in pediatric clinical training was provided for the
5th to 6th graders among 16 months, where every student belonged to one of the professional clinical teams(Hematology/Oncology/Immunology, Cardiology, Neurology, Endocrinology, Neonatology and Nephrology) in
the University Hospital or some aﬃliated hospitals (Tsuchiura Kyodo General Hospital, Kawaguchi Municipal
Medical Center or North Tokyo Medical Center), and studied clinical practice as one of the team members.
Our educational duty is not only for the students of medical school but for them of dental school and healthscience school. We provide lectures on general pediatrics for each of their students several times per year. Juniorclinical fellows who are in the training course of pediatric practice under the supervision of senior staﬀs werealso expected to supervise these medical students. The style of clinical training was maintained and the 1styear trainee as well as the 2nd year trainee could choose the training in the pediatric ward for two months.
On the other hand, the 2nd year trainee was in general engaged in the basic training for one month in thepediatric ward in some aﬃliated hospitals (Musashino Red Cross Hospital, Soka Municipal Hospital or North
Tokyo Medical Center). Depending on the individuals, they could select the advanced training at the pediatricward in The University Hospital for two to eight months.
(4)

Lectures & Courses

It is a goal of education for the 3rd and 4th graders (ﬁrst half) of medical students to learn the whole pictureof general pediatric diseases, and for the 4th graders (latter half, so-called project semester) to touch the basic
research, get the fundamental way of thinking and skills of experiments. On the other hand, it is a goal forthe 5th and 6th graders (so-called pre-Clerkship and Clinical Clerkship), to be in charge of each patient withpediatric staﬀs and experience the general steps under the clinical medicine, for example, the following steps
how to interview the medical history, get the physical ﬁndings, plan the laboratory examinations, diﬀerentiallydiagnose by analyzing the personal data, describe the clinical records, and discuss about the treatment planning.
Moreover, we educate the students of dentistry and health care sciences, who learn not only general pediatricdiseases but the importance of pediatrics as playing roles of total coordination and mutual cooperation beyond
specialty for children s care.
Junior clinical trainees, previously started to train the pediatrics from the 2nd year, became to be able toelect the pediatric training for two months from the 1st year, actually however, the fellows who desired tooptionally choose the pediatric training did not necessarily perform it because of too many applicants. The
2nd year junior clinical trainees were divided two groups. Those only required pediatric training for one
month were generally planned to experience the common pediatric diseases in the aﬃliated hospitals. On theother hand, those electively selected pediatrics were basically planned to train almost in university hospitals
together with at the aﬃliated hospitals for one month. Senior clinical trainees were rotated among in theuniversity hospitals and chief aﬃliated hospitals, planned to experience all kinds of pediatric diseases related tooncology, cardiology, neurology, infections and immunology, endocrinology and metabolic diseases, neonatology,
nephrology, pulmonology, digestive diseases, and genomics.
(5)

Clinical Services & Other Works● Hematology/Oncology/Immunology Group
Hematology/Oncology/Immunology Group treats children with primary immunodeﬁciency, hematological malignancies, hematological disorders, and malignant solid tumors. Our team consists of 9 staﬀs, including 5
senior with diplomate of board of pediatrics, hematology, and/or pediatric hematology/oncology and 4 juniorstaﬀs. We oﬀer a team-based high-quality and evidence-based clinical care for both inpatients and outpatients.
Additionally, we have a cooperative system for medical liaison with other professional facilities including St.
Luke s International Hospital and Juntendo University Hospital; joint clinical conference and trainee exchange
program has been ongoing..
1. Participation in multi-center cooperative clinical research group: In collaboration with national co-operative
clinical research group, such as the Tokyo Children s Cancer Study Group (TCCSG) and Japanese Childrens Cancer Study Group (JCCG), we oﬀer our patients opportunities to participate in the latest clinical trials
and contribute to establish both standard and novel therapies for childhood cancers and other non-malignant
diseases.
2. Number of newly admitted patient was 37 (15 PID, 14 hematological malignancy and 2 solid tumor)
Hematopoietic cell transplantation (HCT): In 2016, we performed HCT for 10 cases; related bone marrow transplantation (BMT) (n=2) (including 2 haploid identical or non sibling donor source), unrelated BMT (n=6),― 274 ―

Bio-Environmental Response

Bio-Environmental Response

unrelated peripheral blood stem cell transplantation (PBSCT) (n=1), unrelated cord blood transplantation(n=1). 6 cases of them were for PID patients. Our experience of HCT exceeds 172 cases including more than
65 cases with primary immunodeﬁciency diseases, so far. We are also working on novel HCT methods, such astransplantation with haplo-identical donor and killer inhibitory receptor (KIR) ligand mismatched donor, anduse of reduced-intensity conditioning aiming for reduction of late eﬀects in HSCT recipients.
3. Three Investigator oriented clinical trials were performed. Eﬃcacy and safety of zoledoronate for RALD.
HCT for defective DNA damage response associated primary immunodeﬁciencies.
4. long-term follow-up for childhood cancer survivors (CSS): In cooperation with pediatric endocrionologists,
CLS (child life specialist) and psychotherapists, we are taking care of cancer survivors and supporting their
quality of life.
● Cardiology GroupThe University Hospital has been certiﬁed as a training institute to produce Board Certiﬁed Pediatric Cardiologist recognized by Japanese Society of Pediatric Cardiology and Cardiac Surgery. In TMDU aﬃliatedhospitals, there were 16 Board Certiﬁed Pediatric Cardiologists. In 2016 there were four full-time doctors, one
Board Certiﬁed Pediatric Cardiologist (Doi S, MD, PhD) and three pre-Board Certiﬁed Pediatric Cardiologists(Komiya E, MD, Yamaguchi Y, MD, Maeda Y, MD) in The University Hospital, who were engaged in the
diagnosis and treatment for every kind of heart disease patients both in the pediatric ward and the ﬁeld forpediatric outpatients. Another Board Certiﬁed Pediatric Cardiologist (Izumida N, MD, PhD) helped to see theoutpatients as a part-time doctor. Dr. Yamaguchi Y were belonged to be a staﬀ of pediatric cardiovascularsurgery.
The hospitalization number of patients was 102, which was a little bit decreased compared to the last year
and the average length of hospital stay was 7.9 days, which was shorter than the last year. The diseases ofadmitted patients were 59 CHD, acquired heart diseases such as 12 PH, 14 cardiac arrhythmias, 10 Kawasaki
diseases (KD), and seven another disease. Forty-ﬁve cardiac catheterizations were performed, which contained
31 CHD, 8 PH, 5 KD and 1 cardiac arrhythmias. Catheter interventions were performed on one patient who
was ventricular tachycardia for the ﬁrst time in pediatric cardiology group. Twenty CHD patients (9 VSDs, 9
ASDs, one TOF and one cAVSD) were surgically operated. Infants under 1 year old was 6 with 4 PAH. PA
banding operation, which is classiﬁed in Risk category 3, was performed on cAVSD with Down syndrome inthis year.
PH patients were admitted for diagnosis, evaluation of treatments or decision of treatment strategy. The mostimportant thing is early diagnosis and early initiation of treatment for PH, which is nominated for diﬃcult-cured
and progressive disease. Therefore, we decided to positively treat by receiving up-front combination therapy(uCT) with three kinds of disease targeted drugs and inducing continuous venous infusion of epoprostenol.
Four patients were treated with uCT and one patient was induced epoprostenol. As the result, we succeeded indecreasing pulmonary arterial pressure as well as increase in cardiac output and decrease in pulmonary vascular
resistance. We also started to use a couple of new speciﬁc medications on PH such as subcutaneous treprostinil,
oral macitentan and riociguat. Among them, eﬀective subcutaneous trprostinil was induced for more two patients. As the result, three pediatric patients were induced subcutaneous trprostinil in TMDU which was 60%
in Japan.
Out-patients for pediatric cardiology were up to 2,000 patients with the 1,500 examinations of echocardiogram.
Moreover, Holter 24-hours ECG monitoring examination and Treadmill exercise tolerance examination wererespectively performed on about 100 patients.
We have participated in the school heart screening program of Tokyo Metropolitan Institute for Preventive
Medicine and Tokyo Medical Association, and checked 9,000 students ECG records in elementary, junior highand senior high schools. The students who were needed the third stage checkup visited The University Hospital,
examined at out- or in-patients ﬁelds and ﬁnally decided the exercise restriction level in school life.
● Neurology GroupChild neurology group provides highly specialized diagnostic approach and medical care for neurological disorders such as epilepsy, neuromuscular disorders, infection of nervous system, neurodegenerative diseases andgenetic syndromes. In particular, in cooperation with the department of neurosurgery, we evaluate the indication for surgical treatment and then perfome surgical operation such as focal brain resection to the patient of
intractable epilepsy.
● Endocrinology GroupWe provide highly speciﬁc diagnostic approach and therapy for pediatric endocrine disorders, such as growth retardation, hypogonadism, thyroid diseases, disorder of sex development (DSD), disorder of Ca-P-PTH metabolism,― 275 ―

Bio-Environmental Response

Bio-Environmental Response

type1 diabetes mellitus. In collaborating with the satellite hospitals, we are following more than a thousand
patients, and the annual number of inpatients with endocrine disorder of our university is more than 100.
Senior physician of our group is an adviser of Tokyo Health Service Association, and supervising the newborn
screening of congenital adrenal hyperplasia in Tokyo.
Among many pediatric endocrine disorders, we are directing our eﬀort at the disorders of adrenal gland and sex
development (DSD), and looking at establishing the clinical center for those patients with pediatric-urologist
and other co-medical staﬀs.
● Nephrology Group
Nephrology Group provides diagnosis and treatment for patients with various kidney disease: acute and chronic
glomerular diseases, nephrotic syndrome, chronic kidney disease, congenital abnormality of kidney and urinary
tract, and acute kidney injury that requires blood puriﬁcation. We perform kidney biopsy and imaging examination for diagnosis in our hospital. We willingly participate in urinary analysis screening for school children.
This year, we treated children with congenital nephrotic syndrome, refractory nephrotic syndrome, IgA nephropathy, acute kidney injury, chronic kidney disease, vesicoureteral reﬂux, and so on. We performed peritoneal
dialysis for low-body-weight children and provided acute hemodialysis for children who developed acute kidney
injury. We performed kidney biopsy about 40 cases a year. Group conference is held on 4th Thursday every
month for lecture and discussion about the cases we experienced. Graduate students present their researchat the conference to share research progress. We participate to some conferences that pediatric nephrologists
from other institutions gather, that give us the valuable opportunities to discuss the critical cases with otherspecialists.
Some members of our group receive training for pediatric nephrologists at National Research Institute for Child
Health to improve their skills.
We present case reports of our patients and research reports in several major conferences such as the meetingof Japanese society of nephrology and the meeting of Japanese society of pediatric nephrology.
● Neonatology group1) Our NICU (Neonatal Intensive Care Unit) was established on April 2012 with 6 beds, and provide intensive
care for preterm infants (> 28 weeks of gestation and/or > 1000g of birth weights). We also take care of
critically ill newborns, those with congenital heart disease, hematological disorder, etc., in cooperation withother pediatric subspecialty groups.
2) As a designated Perinatal Cooperation Hospital in Tokyo, we accept newborn patients from various areas inTokyo.
● Allergy GroupThe qualiﬁed allergists of the group attend both inpatient and outpatient care units for allergic diseases inaﬃliated hospitals of our university, where not only the standard medical services following clinical guidelines
for allergic diseases are provided, but also highly advanced treatment such as oral immunotherapy for foodallergy as well.
We conduct public educational activities about allergic diseases, organizing speciﬁc lecture meetings in cooperation with local public health center and/or medical societies.

(6)

Clinical Performances● Hematology-Oncology/ Immunology Group
Hematology-Oncology/ Immunology Group provides diagnosis, treatment and pathological analysis of hematological malignancies and primary immunodeﬁciency diseases. We perform hematopoietic stem cell transplantation for refractory diseases. Especially, we treat the largest number of primary immunodeﬁciency disease
patients in Japan. We participate in multi-center cooperative clinical research to establish both standard andnovel therapies for childhood cancers, and also participate in industry-based clinical trials for drug, such as
anticancer drug, approval.
● Cardiology Group
Cardiology group perform diagnosis, evaluation of treatment or decision of treatment strategy for PH patients.
We positively treat by up-front combination therapy (uCT) with three kinds of disease targeted drugs andcontinuous venous infusion of epoprostenol. Worthy of special mention, surgical operation in children withcongenital heart diseases was restarted after about 30 years blank.

― 276 ―

Bio-Environmental Response

Bio-Environmental Response● Neurology Group
Neurology group provide highly specialized diagnostic approach and medical care for neurological disorders
such as epilepsy, neuromuscular disorders, nervous system infections, neurodegenerative diseases and genetic
syndromes.
● Endocrinology GroupThe leader of our endocrinology group is a supervisor of congenital adrenal hyperplasia (CAH) newborn screening in Tokyo. We treat many CAH (21-OHD) patients and performed couples of clinical studies. We also focus
on disorder of sex development (DSD) and long-term follow-up for childhood cancer survivors (CSS). We aremanaging a Type 1 DM patients association (Wakamatsu-kai) and organize the summer camp every year.
● Nephrology GroupWe treat various pediatric kidney diseases, such as congenital nephrotic syndrome, refractory nephrotic syndrome, IgA nephropathy, etc. Kidney biopsy is performed to more than 40 patients. We provide acute hemodialysis treatment and peritoneal dialysis for low-body-weight patients (under 10kg) in cooperation with departmentof blood puriﬁcation.
● Neonatology Group
Our NICU (Neonatal Intensive Care Unit) provides intensive care for preterm infants and critically ill newborns.
As a designated Perinatal Cooperation Hospital in Tokyo, we accept newborn patients from various areas inTokyo by collaborating with comprehensive reproductive medicine.
● Allergy GroupWe focus on clinical care of severe and complicated allergic diseases such as food allergy-induced anaphylaxis,
food-dependent exercise-induced anaphylaxis, food protein-induced enterocolitis syndrome and oral allergy syndrome induced by cross-reactivity among food, inhalant and contact allergens. We extensively perform foodchallenge tests not only for correct diagnosis of food allergy but for preparation of oral immunotherapy incooperation with the aﬃliated hospitals.

(7)

Publications[Original Articles]
1. T Wada, Toma T, M Yasui, M Inoue, K Kawa, K Imai, T Morio, A Yachie. Diﬀerent Clinical Phenotypes
in 2 Siblings With X-Linked Severe Combined Immunodeﬁciency. J Investig Allergol Clin Immunol. 2016;
26(1); 63-65
2. Tomohiro Kohmoto, Atsumi Tsuji, Kei-Ichi Morita, Takuya Naruto, Kiyoshi Masuda, Kenichi Kashimada,
Keisuke Enomoto, Tomohiro Morio, Hiroyuki Harada, Issei Imoto. A novel COL11A1 missense mutationin siblings with non-ocular Stickler syndrome. Hum Genome Var. 2016; 3; 16003
3. Kotaro Kai, Maki Sumida, Yaeko Motoyoshi, Yuichi Ogawa, Katsuyuki Miki, Kazuhiro Iwadoh, Akihito
Sannomiya,ToruMurakami,IchiroKoyama,KumikoKitajima,IchiroNakajima,TomohiroMorio,ShoheiFuchinoue. Successful Long-term Graft Survival of a Renal Transplantation Patient with WiskottAldrichSyndrome. Intern. Med. 2016; 55(13); 1761-1763
4. Saito Y, Nishida N, Nomura K, Adachi Y, Kanegane H.. A case of myeloid sarcoma followed by acute
myeloid leukemia (AMIL M0). J Hematol Oncol. 2016; 53(3); 300-304
5. Mami Miyado, Masafumi Inui, Maki Igarashi, Yuko Katoh-Fukui, Kei Takasawa, Akiko Hakoda, Junko
Kanno, Kenichi Kashimada, Kenji Miyado, Moe Tamano, Tsutomu Ogata, Shuji Takada, Maki Fukami.
The p.R92W variant of NR5A1/Nr5a1 induces testicular development of 46,XX gonads in humans, but
not in mice: phenotypic comparison of human patients and mutation-induced mice. Biol Sex Diﬀer. 2016;
7; 56
6. Yuki Aoki, Reiji Miyawaki, Kohsuke Imai, Masatoshi Takagi, Michiko Kajiwara, Yasuyoshi Ishiwata,
Masato Yasuhara, Tomohiro Morio, Shuki Mizutani, Daisuke Tomizawa. Haploidentical Bone Marrow
TransplantationWithClofarabineandBusulfanConditioningforaChildWithMultipleRecurrentAcute
Lymphoblastic Leukemia. J. Pediatr. Hematol. Oncol. 2016.01; 38(1); 39-41― 277 ―

Bio-Environmental Response

Bio-Environmental Response

7. Seiichi Hayakawa, Satoshi Okada, Miyuki Tsumura, Sonoko Sakata, Yoshitaka Ueno, Kohsuke Imai,TomohiroMorio,OsamuOhara,KazuakiChayama,MasaoKobayashi.APatientwithCTLA-4HaploinsuﬃciencyPresentingGastricCancer.J.Clin.Immunol.2016.01;36(1);28-32
8. Laura Gámez-Díaz, Dietrich August, Polina Stepensky, Shoshana Revel-Vilk, Markus G Seidel, Mitsuiki
Noriko,TomohiroMorio,AustenJJWorth,JacobBlessing,FrankVandeVeerdonk,TobiasFeuchtinger,Maria Kanariou, Annette Schmitt-Graeﬀ, Sophie Jung, Suranjith Seneviratne, Siobhan Burns, Bernd H
Belohradsky, Nima Rezaei, Shahrzad Bakhtiar, Carsten Speckmann, Michael Jordan, Bodo Grimbacher.
The extended phenotype of LPS-responsive beige-like anchor protein (LRBA) deﬁciency. J. Allergy Clin.
Immunol.2016.01;137(1);223-230
9. T Muto, Y Takeda, S Tsukamoto, S Sakai, N Mimura, C Ohwada, M Takeuchi, E Sakaida, S Ota, T
Iseki, N Shimizu, T Morio, C Nakaseko. Successful treatment of cytomegalovirus enteritis after unrelated
allogeneic stem cell transplantation by the infusion of ex vivo-expanded CD4(+) lymphocytes derived
from the recipient’s peripheral blood donor cells. Transpl Infect Dis. 2016.02; 18(1); 93-97
10. KeiTakasawa,SayakaTakeda,MasatoNishioka,HiroshiSakuma,TomohiroMorio,MasayukiShimohira.
Steroid-responsive Status Epilepticus Caused by Human Parvovirus B19 Encephalitis. Pediatr. Infect.
Dis. J. 2016.02; 35(2); 227-228
11. Yumiko Nakano, Takeshi Hayashi, Kentaro Deguchi, Kota Sato, Nozomi Hishikawa, Toru Yamashita,Yasuyuki Ohta, Yoshiki Takao, Tomohiro Morio, Koji Abe. Two young stroke patients associated
withregular intravenous immunoglobulin (IVIg) therapy. J. Neurol. Sci. 2016.02; 361; 9-12
12. Kazuhiro Ikegame, Kohsuke Imai, Motoi Yamashita, Akihiro Hoshino, Hirokazu Kanegane, Tomohiro
Morio, Katsuji Kaida, Takayuki Inoue, Toshihiro Soma, Hiroya Tamaki, Masaya Okada, Hiroyasu Ogawa.
Allogeneic stem cell transplantation for X-linked agammaglobulinemia using reduced intensity conditioning
as a model of the reconstitution of humoral immunity. J Hematol Oncol. 2016.02; 9(1); 9
13. AArai,CSakashita,CHirose,K-IImadome,MYamamoto,MJinta,SFujiwara,MTomita,NShimizu,T Morio, O Miura. Hematopoietic stem cell transplantation for adults with EBV-positive T- or NK-cell
lymphoproliferative disorders: eﬃcacy and predictive markers. Bone Marrow Transplant. 2016.02; 51(6);
879-882
14. Amit Rawat, Kohsuke Imai, Deepti Suri, Anju Gupta, Swapnil Bhisikar, Biman Saikia, Ranjana W Minz,
Shobha Sehgal, Surjit Singh. Ataxia Telangiectasia Masquerading as Hyper IgM Syndrome. Indian J
Pediatr. 2016.03; 83(3); 270-271
15. Katsutsugu Umeda, Souichi Adachi, Yasuo Horikoshi, Kohsuke Imai, Kiminori Terui, Mikiya Endo, Tetsuo
Mitsui, Keisuke Kato, Katsuyoshi Koh, Ryosuke Kajiwara, Rieko Ito, Yoshitoshi Otsuka, Masami Inoue,
Eiichi Ishii, Hiromasa Yabe. Allogeneic hematopoietic stem cell transplantation for Chediak-Higashi
syndrome. Pediatr Transplant. 2016.03; 20(2); 271-275
16. Hiroshi Moritake, Masatoshi Takagi, Mariko Kinoshita, Osamu Ohara, Shojiro Yamamoto, Sayaka Moriguchi,
Hiroyuki Nunoi. Autoimmunity Including Intestinal Behçet Disease Bearing the KRAS Mutation in Lymphocytes: A Case Report. Pediatrics. 2016.03; 137(3); 1-5
17. Masakatsu Yanagimachi, Takashi Ohya, Tomoko Yokosuka, Ryosuke Kajiwara, Fumiko Tanaka, Hiroaki
Goto, Takehiro Takashima, Tomohiro Morio, Shumpei Yokota. The Potential and Limits of Hematopoietic
Stem Cell Transplantation for the Treatment of Autosomal Dominant Hyper-IgE Syndrome. J. Clin.
Immunol.. 2016.04; 36(5); 511-516
18. Julie Toubiana, Satoshi Okada, Julia Hiller, Matias Oleastro, Macarena Lagos Gomez, Juan Carlos Aldave Becerra, Marie Ouachée-Chardin, Fanny Fouyssac, Katta Mohan Girisha, Amos Etzioni, Joris Van
Montfrans, Yildiz Camcioglu, Leigh Ann Kerns, Bernd Belohradsky, Stéphane Blanche, Aziz Bousﬁha,
Carlos Rodriguez-Gallego, Isabelle Meyts, Kai Kisand, Janine Reichenbach, Ellen D Renner, Sergio Rosenzweig, Bodo Grimbacher, Frank L van de Veerdonk, Claudia Traidl-Hoﬀmann, Capucine Picard, Laszlo
Marodi, Tomohiro Morio, Masao Kobayashi, Desa Lilic, Joshua D Milner, Steven Holland, Jean-Laurent
Casanova, Anne Puel. Heterozygous STAT1 gain-of-function mutations underlie an unexpectedly broadclinical phenotype: an international survey of 274 patients from 167 kindreds. Blood. 2016.04; 127(25);3154-3164― 278 ―

Bio-Environmental Response

Bio-Environmental Response

Mitsuiki, Akifumi Endo, Yuki Aoki, Toshiaki
Ono,
19. Chika Kobayashi, Masayuki Nagasawa, Noriko
Kohsuke Imai, Masatoshi Takagi, Michiko Kajiwara, Tomohiro Morio.. Recombinant Human SolubleThrombomodulin is Eﬀective for Severe Childhood EBV-HLH. 2016.05; 2(1); 1-4
20. Yuki Tsujita, Kanako Mitsui-Sekinaka, Kohsuke Imai, Tzu-Wen Yeh, Noriko Mitsuiki, Takaki Asano, Hidenori Ohnishi, Zenichiro Kato, Yujin Sekinaka, Kiyotaka Zaha, Tamaki Kato, Tsubasa Okano, Takehiro
Takashima, Kaoru Kobayashi, Mitsuaki Kimura, Tomoaki Kunitsu, Yoshihiro Maruo, Hirokazu Kanegane, Masatoshi Takagi, Kenichi Yoshida, Yusuke Okuno, Hideki Muramatsu, Yuichi Shiraishi, Kenichi
Chiba, Hiroko Tanaka, Satoru Miyano, Seiji Kojima, Seishi Ogawa, Osamu Ohara, Satoshi Okada, Masao
Kobayashi, Tomohiro Morio, Shigeaki Nonoyama. Phosphatase and tensin homolog (PTEN) mutation
can cause activated phosphatidylinositol 3-kinase δ syndrome-like immunodeﬁciency. J. Allergy Clin.
Immunol.. 2016.06; 138(6); 1672-1680
21. Takasawa K, Miyakawa Y, Masamune A, Kashimada K, Shimohira M. Fibrocalculous pancreatic diabetes
in a Japanese girl with severe motor and intellectual disabilities. Acta Diabetologica. 2016.06; 53(3);507-510
22. Elodie Elkaim, Benedicte Neven, Julie Bruneau, Kanako Mitsui-Sekinaka, Aurelie Stanislas, Lucie Heurtier,
Carrie L Lucas, Helen Matthews, Marie-Céline Deau, Svetlana Sharapova, James Curtis, Janine Reichenbach, Catherine Glastre, David A Parry, Gururaj Arumugakani, Elizabeth McDermott, Sara Sebnem Kilic,
Motoi Yamashita, Despina Moshous, Hicham Lamrini, Burkhard Otremba, Andrew Gennery, Tanya Coulter, Isabella Quinti, Jean-Louis Stephan, Vassilios Lougaris, Nicholas Brodszki, Vincent Barlogis, Takaki
Asano, Lionel Galicier, David Boutboul, Shigeaki Nonoyama, Andrew Cant, Kohsuke Imai, Capucine
Picard, Sergey Nejentsev, Thierry Jo Molina, Michael Lenardo, Sinisa Savic, Marina Cavazzana, Alain
Fischer, Anne Durandy, Sven Kracker. Clinical and immunologic phenotype associated with activatedphosphoinositide 3-kinase δ syndrome 2: A cohort study. J. Allergy Clin. Immunol.. 2016.07; 138(1);210-218.e9
23. Shima H, Tanaka T, Kamimaki T, Dateki S, Muroya K, Horikawa R, Kanno J, Adachi M, Naiki Y, Tanaka
H, Mabe H, Yagasaki H, Kure S, Matsubara Y, Tajima T, Kashimada K, Ishii T, Asakura Y, Fujiwara
I, Soneda S, Nagasaki K, Hamajima T, Kanzaki S, Jinno T, Ogata T, Fukami M; Japanese SHOX study
group.. Systematic molecular analyses of SHOX in Japanese patients with idiopathic short stature andLeri-Weill dyschondrosteosis. Journal of human genetics. 2016.07; 61(7); 585-591
24. Shigeru Takishima, Keisuke Nakajima, Risa Nomura, Atsumi Tsuji-Hosokawa, Nozomi Matsuda, Yohei
Matsubara, Makoto Ono, Kentaro Miyai, Kei Takasawa, Tomohiro Morio, Yukihiro Hasegawa, Kenichi
Kashimada. Lower body weight and BMI at birth were associated with early adiposity rebound in 21hydroxylase deﬁciency patients. Endocr. J.. 2016.08; 63(11); 983-990
25. Katsutsugu Umeda, Souichi Adachi, Shiro Tanaka, Mizuka Miki, Keiko Okada, Yoshiko Hashii, Masami
Inoue, Yuko Cho, Katsuyoshi Koh, Hiroaki Goto, Ryosuke Kajiwara, Nobuyuki Hyakuna, Koji Kato,
Tomohiro Morio, Hiromasa Yabe, . Comparison of second transplantation and donor lymphocyte infusion
for donor mixed chimerism after allogeneic stem cell transplantation for nonmalignant diseases. Pediatr
Blood Cancer. 2016.08; 63(12); 2221-2229
26. Takuya Wakamiya, Tatsunori Hokosaki, Shin-Ichi Tsujimoto, Keisuke Kadota, Yusuke Nakano, Shigeo
Watanabe, Mari Iwamoto, Masakatsu Yanagimachi, Shuichi Ito. Eﬀect of VKORC1, CYP2C9, CFP4F2,
and GGCX Gene Polymorphisms on Warfarin Dose in Japanese Pediatric Patients. Mol Diagn Ther.
2016.08; 20(4); 393-400
27. A Hayashi, R Tanoshima, S-I Tsujimoto, M Yanagimachi, M Takeuchi, K Sasaki, J Ikeda, R Kajiwara, S
Ito, H Takahashi. Crizotinib treatment for refractory pediatric acute myeloid leukemia with RAN-binding
protein 2-anaplastic lymphoma kinase fusion gene. Blood Cancer J. 2016.08; 6(8); e456
28. Midori Yukizawa, Masakatsu Yanagimachi, Koji Sasaki, Reo Tanoshima, Hiromi Kato, Tomoko Yokosuka,
Ryosuke Kajiwara, Fumiko Tanaka, Hiroaki Goto, and Shumpei Yokota. Evaluation of infection before
hematopoietic stem cell transplantation for patients with primary immunodeﬁciency diseases by FDG-PETThe Journal of the Japan Pediatric Society. 2016.08; 120(8); 1220-1226
29. Shumpei Yokota, Yasuhiko Itoh, Tomohiro Morio, Hideki Origasa, Naokata Sumitomo, Minako Tomobe,
Kunihiko Tanaka, Seiji Minota. Tocilizumab in systemic juvenile idiopathic arthritis in a real-world
clinicalsetting: resultsfrom1yearofpostmarketingsurveillancefollow-upof417patientsinJapan. Ann.
Rheum. Dis. 2016.09; 75(9); 1650-1660― 279 ―

Bio-Environmental Response

Bio-Environmental Response

30. Takeshi Sato, Tsubasa Okano, Mari Tanaka-Kubota, Shunsuke Kimura, Satoshi Miyamoto, Shintaro Ono,
Motoi Yamashita, Noriko Mitsuiki, Masatoshi Takagi, Kohsuke Imai, Michiko Kajiwara, Takasuke Ebato,
Shohei Ogata, Hirotsugu Oda, Osamu Ohara, Hirokazu Kanegane, Tomohiro Morio. Novel compound
heterozygous mutations in a Japanese girl with Janus kinase 3 deﬁciency. Pediatr Int. 2016.09; 58(10);1076-1080
31. Akaike S, Kamoi K, Tezsuka M, Tomizawa D, Yoshimura R, Takagi M, Ohno-Matsui K. Ocular manifestation in myeloid/NK cell precursor acute leukemia: a case report. Diagnosed by ﬂow cytometry and
PCR from aqueous humor. Medicine (Baltimore). 2016.09; 95(38); e4967
32. Surjit Singh, Amit Rawat, Deepti Suri, Anju Gupta, Ravinder Garg, Biman Saikia, Ranjana WalkerMinz, Shobha Sehgal, Koon-Wing Chan, Yu Lung Lau, Chikako Kamae, Kenichi Honma, Noriko Nakagawa, Kohsuke Imai, Shigeaki Nonoyama, Koichi Oshima, Noriko Mitsuiki, Osamu Ohara. X-linked
agammaglobulinemia: Twenty years of single-center experience from North West India. Ann.
AllergyAsthma Immunol. 2016.10; 117(4); 405-411
33. DaieiKojima,HidekiMuramatsu,YusukeOkuno,ShinsukeKataoka,NorihiroMurakami,YoshihiroTanahashi, Kyogo Suzuki, Tamaki Kato, Yuko Sekiya, Nozomu Kawashima, Atsushi Narita, Nobuhiro Nishio,
Asahito Hama, Kohsuke Imai, Shigeaki Nonoyama, Yoshiyuki Takahashi, Seiji Kojima. Successful T-cell
reconstitution after unrelated cord blood transplantation in a patient with complete DiGeorge syndrome.
J. Allergy Clin. Immunol. 2016.11; 138(5); 1471-1473.e4
34. Minoru Asada, Shuki Mizutani, Masatoshi Takagi, Hidenori Suzuki. Antipsychotics promote neural differentiation of human iPS cell-derived neural stem cells. Biochem. Biophys. Res. Commun. 2016.11;
480(4); 615-621
35. Shin-Ichi Tsujimoto, Masakatsu Yanagimachi, Reo Tanoshima, Kevin Y Urayama, Fumiko Tanaka, Noriko
Aida, Hiroaki Goto, Shuichi Ito. Inﬂuence of ADORA2A gene polymorphism on leukoencephalopathy risk
in MTX-treated pediatric patients aﬀected by hematological malignancies. Pediatr Blood Cancer. 2016.11;
63(11); 1983-1989
36. Hiroshi Moritake, Megumi Obara, Yusuke Saito, Ayako Kashimada, Masatoshi Takagi, Megumi FunakoshiTago, Tomofusa Fukuyama, Mikio Yoshioka, Akira Inoue, Hiroyuki Komatsu, Hideki Nishitoh, Hiroaki
Kataoka, Hiroyuki Nunoi. A mouse model reveals that Mfsd2a is critical for unfolded protein response
upon exposure to tunicamycin. Hum. Cell. 2016.11;
37. Akifumi Endo, Daisuke Tomizawa, Yuki Aoki, Tomohiro Morio, Shuki Mizutani, Masatoshi
Takagi.EWSR1/ELF5 induces acute myeloid leukemia by inhibiting p53/p21 pathway. Cancer Sci.
2016.12;107(12); 1745-1754
38. Mayu Ishibashi, Tomoko Yokosuka, Masakatsu D Yanagimachi, Fuminori Iwasaki, Shin-Ichi Tsujimoto,Koji Sasaki, Masanobu Takeuchi, Reo Tanoshima, Hiromi Kato, Ryosuke Kajiwara, Fumiko Tanaka,
Hiroaki Goto, Shumpei Yokota. Clinical Courses of Two Pediatric Patients with Acute Megakaryoblastic
Leukemia Harboring the CBFA2T3-GLIS2 Fusion Gene. Turk J Haematol. 2016.12; 33(4); 331-334[Misc]
1. Masatoshi Takagi, Kevin Urayama. Inherited genetic variants associated with childhood lymphoblastic
leukemia risk. The Japanese Journal of Clinical Hematology. 2016.07; 57(7); 891-899[Conference Activities & Talks]
1. MikhailRojavin,HirokazuKanegane,MichaelBorte,Imaik,AlphonseP.Hubsch,HelenaSoop,StephenR. Jolles. Pooled Analysis of Patient Treatment Satisfaction from Five Hizentra Studies. 2016 AAAAIANNUAL MEETING 2016.03.04 Los Angeles 64"
2. Imai K. HYPER-IGM SYNDREOMS DUE TO CSR DEFECTS-HOW TO TREAT ACCORDING TO
GENETIC SUBTYPES?. ASPID Spring School 2016.04.27 HongKong $IBJOB― 280 ―

Bio-Environmental Response

Bio-Environmental Response

3. Imai K. PIDJ AND PIER-PID DATABASE IN JAPAN FOR DOCTORS AND PATIENTS-HOW IT
FACILITATES CARE AND RESEARCH?. ASPID Spring School 2016.04.27 HongKong
4. Kanegane H. Use of Flow Cytometry in PID diagnosis. APSID Spring School 2016.04.28 Hong Kong
5. Kanegane H. Immunoglobulin Replacement for Agammaglobulinemia – Intravenous or Subcutaneous?.
ASPID Spring School 2016.05.01 HongKong $IBJOB
6. Okano T, Imai K, Tsujita Y, Mitsuiki , Mitsui-Sekinaka K, Takashima T, Kanegane H, Nonoyma S.
Broad Spectrum of Clinical Manifestations and Outcome of Hematopoietic Stem Cell Transplantation in
Japanese Patients with Activated P13KD Syndrome Type1. ASPID Spring School 2016.05.01 Hong Kong
7. Akihiro Hoshino, Hirokazu Kanegane. ABNORMAL HEMATOPOIESIS AND AUTOIMMUNITY IN
HUMANS WITH GERMLINE IKZF1 MUTATIONS. 2016.05.27
8. Doi S. Congenital Heart Disease associated with Pulmonary Arterial Hypertension.. Panal Discussion.
Riociguat Meet the Experts 2016.05.27 Tokyo  Japan
9. Kengo Moriyama, Shinpei Baba, Kazue Kimura, Yuji Sugawara. Clinical consideration of six cases ofAngelman syndrome. The 58th Annual Meeting of the Japanese Society of Child Neurology 2016.06.04
Toky +BQBO
10. Inﬂuence of ABCB1 polymorphisms with drug-responsiveness to Japanese children with epilepsy. 2016.06.05
11. Masatoshi Takagi, Misa Yoshida, Yoshino Nemoto, Hiroyuki Tamaichi, Rika Tsuchida, Masafumi Seki,
Kumiko Uryu, Noriko Hoshino, Rina Nishii, Satoshi Miyamoto, Masahiro Saito, Toshiaki Shimizu, Ryoji
Hanada, Hideo Kaneko, Toshiyuki Fukao, Takatoshi Koyama, Yuichi Shiraishi, Kenichi Chiba, Hiroko
Tanaka, Satoru Miyano, Yusuke Sato Sato, Keisuke Kataoka, Yusuke Okuno, Kenichi Yoshida, Tomohiro
Morio, Akira Oka, Miki Ohira, Yasuhide Hayashi, Akira Nakagawara, Seishi Ogawa, Shuki Mizutani,
Junko Takita. Loss of ATM function confers risk for advanced stage neuroblastoma but provides atherapeutic target for poly-ADP ribose polymerase inhibitors. Advances in Neuroblastoma Research
Congress 2016 2016.06.21 Queensland, Australia
12. Haruna Shibayama, Ken-ichi Imadome, Chizuko Sakashita, Ken Watanabe, Norio Shimizu, Takatoshi
Koyama, Shigeyoshi Fujiwara, Osamu Miura, Ayako Arai. In vitro and in vivo eﬀects of proteasome
inhibitor bortezomib on Epstein-Barr virus-positive T- or NK-cell lymphoproliferative diseases. 17th
International Symposium on Epstein-Barr virus (EBV) and Associated Diseases 2016.08.10 Zurich
13. Type l interferon response induced by transfected self DNA. 2016.09.03
14. Schwab C, Gabrysch A, Hoshino M, Slatter M, Hayakawa S, Schubert D, Klemann C, Frede N, Salzer U,
Speckmann C, Freiberger T, Lorenz H.M, Hauck F, Klobuch S, Zeissig S, Leichtner A, Van de Veerdonk
F, Elemnto O, Giulino-Roth L, Duckers G, Niehues T, Alsina L, Deya-Martinez A, Kanderiva V, Fronkova
E, Price C, Patino V, Blumberg R, Yesim Kucuk Z, Seneviratne S, Kanariou M, Lougaris V, Albert M,
Giese T, Litzman J, Ehl S, Okada S, Imai K, Dybedai I, Tasken K, Wolﬀ D, Warnatz K, Hambleton S,
Arkwright P, Cant A, Kanegane H, Grimbacher B.. The Clinical Spectrum and Treatment Options of 82
Individuals with CTLA-4Deﬁciency. 17th Biennial Meeting of the European Society for Immunodeﬁciency
2016.09.22 Barcelona, Spain
15. Mitsui-Sekinaka K, Imai K, Tsujita Y, Mitsuiki N, Asano T, Sekinaka Y, Kanegane H, Yoshida K, Miyano
S, Kojima S, Ogawa S, Ohara O, Okada S, Kobayashi M, Takagi M, Morio T, Nonoyama S.. Activated
PI3 Kinase Delta Syndrome (APDS)-Like Immunodeﬁciency Caused by PTEN Mutation. 17th Biennial
Meeting of the European Society for Immunodeﬁciencies 2016.09.22 Barcelona, Spain
16. Hoshino A, Okada S, Yoshida K, Nishida N, Yamashita M, Okano T, Tsumura M, Nishimura S, Sakata
S, Takagi M, Imai K, Miyano S, Ogawa S, Kojima S, Nonoyama S, Morio T, Kanegane H. . Abnormal
Hematopoiesis and Autoimmunity in Humans with Germline IKZF1 Mutations. 17th Biennial Meeting of
the European Society for Immunodeﬁciencies 2016.09.22 Barcelona, Spain
17. Leiding J,Okada S,Shcherbina A,Abinun M,Pulsipher M,Hagin D,Linemans C,Sullivan K,Bunin N,Kilic
S,De La Calle-Martin O,Roiman C,Aldave J.C,Iguchi A,Imai K,Ohkawa T,Gennery A,Slatter M,Ochs
H,Mori T,Torgerson T. Hematopoietic Stem cell Transplantation in Patients with Gain of Function STAT1
Mutation. 17th Biennial Meeting of the European Society for Immunodeﬁciencies 2016.09.22 Barcelona,
Spain― 281 ―

Bio-Environmental Response

Bio-Environmental Response

18. Sekinaka Y,Mitsuiki N,Imai K,Yabe M,Mitsui-Sekinaka K,Honma K,Arai A,Yoshida K,Miyano S,Seiji
K,Hira A,Takata M,Ohara O,Ogawa S,Morio T,Nonoyama S. Common Variable Immunodeﬁciency Caused
by Fanc Mutations. 17th Biennial Meeting of the European Society for Immunodeﬁciencies 2016.09.22
Brcelona, Spain
19. S. Singh, A. Rawat, D. Suri, A. Gupta, R. Garg, B. Saikia, R. Minz, S. Sehga, K. Chan, Y. Lau, C. Kamae, K. Honma, N. Nakagawa, K. Imai, S. Nonoyama, K. Oshima, N. Mitsuiki, O. Ohara. SPECTRUM
OF BTK GENE MUTATIONS IN A SINGLE-CENTRE COHORT OF X-LINKED AGAMMAGLOBULINEMIA FROM NORTH INDIA. 17th Biennial Meeting of the European Society for Immunodeﬁciency
2016.09.22 Barcelona Spain
20. L.A. Henderson, A.E. O’Connell, J.I. Chu, Y.N. Lee, S. Volpi, M. Armant, I.B. Barlan, A. Ozen, S. Baris,
I.C. Hanson, J.M. Despotovic, A. Saitoh, T. Takachi, K. Imai, A. King, S. Arredondo, A. Galy, D.A.
Williams,S.Y. Pai, L.D. Notarangelo. GENE THERAPY USING A SELF-INACTIVATING LENTIVIRAL VECTOR AMELIORATES T CELL RECEPTOR REPERTOIRE ABNORMALITIES IN PATIENTS WITH WISKOTT-ALDRICH SYNDROME. 17th Biennial Meeting of the European Society
for Immunodeﬁciency 2016.09.22 Barcelona Spain
21. Okano T, Watanabe E, Takashima T, Nishikawa T, Kawano Y, Tomoda T, Tanaka-Kubota M, Miyamoto
S, Yeh T, Yamashita M, Tanaka K, Mitusiki N, Ohara O, Takagi M, Imai K, Kanegane H, Morio T..
IGG1 Gammopathy in X-SCID Caused by Maternal T and B cell Engraftment. 17th Biennial Meeting of
the European Society for Immunodeﬁciencies 2016.09.23 Barcelona, Spain
22. Jolles S, Rojavin M, Nelson Jr. R, Wasserman R.L, Borte M, Kanegane H, Imai K.. Eﬃcacy and Safetyof HIZENTRA in Patients with Primary Immunodeﬁciency in Japan, Europe, and the US. 17th Biennial
Meeting of17th Biennial Meeting of the European Society for Immunodeﬁciencies 2016.09.23 Barcelona,
Spain
23. Moriya K, Tanita K, Ohnishi H, Niizuma H, Rikiishi T, Nishikomori R, Ishige T, Imai K, Kanegane
H, Sasahara Y, Arakawa H, Kure S, Morio T. I < kappa> B-α S32 Mutations Underly Ectodermal
Dysplasia with Immunodeﬁciency Manifestations and Correlation with Immunological Parameters.. 17th
Biennial Meeting of the European Society for Immunodeﬁciencies 2016.09.23 Brcelona, Spain
24. Rojavin M, Kanegane H, Borte H, Imai K, Hubsch A, Jolles S.. Analysis of Patient Treatment Satisfaction
from Five Studies of HIZENTRA 20% Subcutaneous Immunoglobulin. 17th Biennial Meeting of theEuropean Society for Immunodeﬁciencies 2016.09.23 Barcelona, Spain
25. T. Mizukami,M. Iwata-Okada, T. Nishimura, T. Kawai, M. Onodera, K. Imai, S. Nonoyama, O. Ohara,
H. Nunoi. CLINICAL AND GENETIC PROFILES OF CHRONIC GRANULOMATOUS DISEASE IN
JAPAN. 17th Biennial Meeting of the European Society for Immunodeﬁciencies 2016.09.23 Barucelona
Spain
26. OnoS,OkanoT,HoshinoA,YanagimachiM,TakagiM,ImaiK,MorioT,KaneganeH.Hematopoietic
stemcelltransplantationforXIAPdeﬁciency.XLP-WAS2016Symposium-London2016.09.26London,UK
27. Kanegane H, Hoshino A, Takashima T, Yeh T-W, Okano T, Yamashita M, Imai K, Morio T. . Selective dysregulation of Epstein Barr virus infection in hypomorphic ZAP70 mutation. XLP-WAS 2016
Symposium-London 2016.09.26 London, UK
28. ImaiK.PrimaryantibodydeﬁciencyinJapan:overviewandP13Krelateddisorders.XLP-WAS2016
Symposium-London2016.09.27London 6,
29. Kanegane H. Recent advances in primary antibody immunodeﬁciency and immunoglobulin replacement
therapy. 21th Congress of Chinese Pediatric Society 2016.10 Zhuhai, PRC
30. HarunaShibayama,Ken-ichiImadome,ChizukoSakashita,KenWatanabe,FuyukoKawano,NorioShimizu,
TakatoshiKoyama,ShigeyoshiFujiwara,OsamuMiura,AyakoArai. InvitroandinvivoeﬀectsofbortezomibonEBV-T/NK-LPDs. The78thAnnualMeetingoftheJapaneseSocietyofHematology2016.10.14
Yokohama +BQBO― 282 ―

Bio-Environmental Response

Bio-Environmental Response

31. Atsumi Tsuji, Kei Takasawa, Risa Nomura, Yuichi Miyakawa, Chikahiko Numakura, Atsushi Hijikata,
Tsuyoshi Shirai, Yoshihiro Ogawa, Kashimada, Tomohiro Morio. Molecular mechanisms of insulin resistance in two cases of primary insulin receptor defect-associated diseases. The 9th biennial scientiﬁcmeeting of the Asia Paciﬁc Paediatric Endocrine Society 2016.10.19 Tokyo, Japan
32. Akihiro Hoshino, Satoshi Okada, Kenichi Yoshida, Naonori Nishida, Yusuke Okuno, Hiroo Ueno, Motoi Yamashita, Tsubasa Okano, Miyuki Tsumura, Shiho Nishimura, Sonoko Sakata, Masatoshi Takagi,
Kohsuke Imai, Yuichi Shiraishi, Kenichi Chiba, Hiroko Tanaka, Satoru Miyano, Seishi Ogawa, Seiji Kojima, Shigeaki Nonoyama, Tomohiro Morio, Hirokazu Kanegane. ABNORMAL HEMATOPOIESIS AND
AUTOIMMUNITY IN HUMANS WITH GERMLINE IKZF1 MUTATIONS. 2016.10.21
33. DoiS.RecentClinicalAdvancesinPediatricPulmonaryHypertension..The6thCongressofAsiaPaciﬁc
PediatricCardiacSociety20162016.10.22Shanghai China
34. Maskatsu Yanagimachi. Genetic background for pediatric rheumatologic disorder. The 26th Annual
Meeting of Pediatric Rheumatology Association of Japan 2016.10.22
35. ImaiK.HematpopoieticstemcelltransplantationforP‘rimary.The21stCongressoftheAsianPaciﬁcSocietyofRespirology(APSR2016)2016.11.11Thailand, Bangkok
36. Tomoda T, Okuno M, Sutani A, Tsuji-Hosokawa A, Imai K, Kashimada K, Urakami T, MorioT. Combined
Therapy with pump and long acting insulin for type 1 diabetes mellitus caused by STAT1 mutation. The
9th Beinnial Scientiﬁc Meeting of the Asia Paciﬁc Paediatric Endocrine Society/ The 50th Annual Meetingof the Japanese Society for Pediatric Endocrinology 2016.11.17 Tokyo, Japan
37. Akito Sutani, Takeru Yamauchi, Ryuichi Nakagawa, Nozomi Matsuda, Yuichi Miyakawa, Risa Nomura,
Atsumi Tsuji-Hosokawa, Shigeru Takishima, Yohei Matsubara, Kei Takasawa, Misako Nagatsuma, Makoto
Ono, Kenichi Kashimada, Tomohiro Morio. Fifty cases of congenital hypothyroidism re-evaluation taken
in our hospital. The 9th Biennial Scientiﬁc Meeting of the Asia Paciﬁc Endocrine Society/ The 50th
Annual Meeting of the Japanese Society for Pediatric Endocrinology 2016.11.17 Tokyo, Japan
38. Maki Igarashi, Kei Takasawa, Akiko Hakoda, Junko Kanno, Shuji Takada, Mami Miyado, Toshihiro
Tajima, Ryohei Sekido, Tsutomu Ogata, Kenichi Kashimada, Maki Fukami. Identical NR5A1 Missense
Mutations in Two Unrelated 46, XX Individuals with Testicular Tissues. The 9th Biennial Scientiﬁc
Meeting of the Asia Paciﬁc Endocrine Society/ The 50th Annual Meeting of the Japanese Society for
Pediatric Endocrinology 2016.11.17 Tokyo, Japan
39. Masahiro Goto, Kazuhisa Akiba, Kenichi Kashimada, Koji Muroya, Masanori Adachi, Tsuyoshi Isojima,
Yukhiro Hasegawa. Infantile body weight gain up to 60 days of age is associated with height at 1 year of
age in classical 21-hydroxylase deﬁciency. The 9th Biennial Scientiﬁc Meeting of the Asia Paciﬁc Endocrine
Society/ The 50th Annual Meeting of the Japanese Society for Pediatric Endocrinology 2016.11.17 Tokyo,
Japan
40. Ryuichi Nakagawa, Akito Sutani, Tsuji Atsumi, Yohei Matsubara, Shigeru Takishima, Kei Takasawa,
Kentaro Miyai, Makoto Ono, Toshikaza Oonishi, Kenichi Kashimada, Tomohiro Morio. The level of LH
and FSH would be a monitoring marker for adult male 21-OHD patient. The 9th Biennial Scientiﬁc
Meeting of the Asia Paciﬁc Endocrine Society/ The 50th Annual Meeting of the Japanese Society for
Pediatric Endocrinology 2016.11.17 Tokyo, Japan
41. Kazuhisa Akiba, Masahiro Goto, Yoshihiko Morikawa, Kenichi Kashimada, Koji Muroya, Masayoshi
Adachi, Yukihiro Hasegawa. Hydrocortisone dose between 180 and 360 days may change Ht- SDS during
the ﬁrst 1 year of life with 21 OHD. The 9th Biennial Scientiﬁc Meeting of the Asia Paciﬁc Endocrine
Society/ The 50th Annual Meeting of the Japanese Society for Pediatric Endocrinology 2016.11.17 Tokyo,
Japan
42. Risa Nomura, Maki Gau, Takeru Yamauchi, Akito Sutani, Atsumi Tsji-Hosokawa, Yohei Matsubara,
Kentaro Miyai, Susumu Hosokawa, Gen Nishimura, Kenichi Kashimada. Myhre syndrome: a progressive
connective tissue disorder with short stature. The 9th Biennial Scientiﬁc Meeting of the Asia Paciﬁc Endocrine Society/ The 50th Annual Meeting of the Japanese Society for Pediatric Endocrinology 2016.11.17
Tokyo, Japan― 283 ―

Bio-Environmental Response

Bio-Environmental Response

43. Atsumi Tsuji, Kei Takasawa, Risa Nomura, Yuichi Miyakawa, Chikahiko Numakura, Atsushi Hijikata,
Tsuyoshi Shirai, YoshihiroOgawa, Kenichi Kashimada, Tomohiro Morio. Molecular mechanisms of insulin
resistance in two cases of primary insulin receptor defect-associated diseases. The 9th Biennial Scientiﬁc
Meeting of the Asia Paciﬁc Endocrine Society/ The 50th Annual Meeting of the Japanese Society for
Pediatric Endocrinology 2016.11.17 Tokyo, Japan
44. Yuichi Miyakwa, Akito Sutani, Atsumi Tsuji, Tomohiro Morio, Kenichi Kashimada. A case of Atypical
Pubertal Development Caused by Dopamine Antagonist. The 9th Biennial Scientiﬁc Meeting of the Asia
Paciﬁc Endocrine Society/ The 50th Annual Meeting of the Japanese Society for Pediatric Endocrinology
2016.11.17 Tokyo, Japan― 284 ―

Bio-Environmental
Bio-Environmental Response
Response

Rheumatology

Professor
Professor emeritus
Professor

Hitoshi KOHSAKA
Nobuyuki MIYASAKA
Masaaki MORI (1)Tetsuo KUBOTA (2)Kazuki TAKADA (3)Ryuji KOIKE (2)Associate Professor
Toshihiro MATSUI (1)Junior Associate Professor Hideyuki IWAI (5)Assistant Professor
Fumitaka MIZOGUCHI, Tetsuya SAITO,
Naoki KIMURA, Hisanori HASEGAWA,
Natsuka UMEZAWA, Waka YOKOYAMA,
Fumio HIRANO (1), Shoko KASAI (4),
Yoko YOSHIHASHI (3)Visiting Lecturer
Nobuyuki MIYASAKA, Hiroyuki HAGIYAMA,
Makoto SOEJIMA,Tatsuhiko SUGIHARA, Fumihiko SUZUKI,
Akito TAKAMURA,Kaori IMAI, Reiko TSUBATA,
Jyunko NISHIO,
Graduate Student
Hirokazu SASAKI, Akio YAMAMOTO,
Tatsuhiko SHINOHARA,Yasuhiro TAGAWA,
Mari Kamiya, Fumiaki KONDO,
Seiji NODA, Taku HONDA
Resident Physician
Wakako KAWASAKI, Yuriko YAGYU, Nao TANAKA,
Yoichi IMAI,Motohiko SATO, Seiya OBA
Oﬃce Administrator
Kaori KONNO, Shoko AIBA, Minako WATANABE,
Tomoko TAKAHASHI (1)Technical Staﬀ
Hidemi KASAHARA, Kazuko YAMAZAKI(1) Department of Lifetime Clinical Immunology, (2) Medical Innovation Promotion Center,(3) Curricular Management Division, Institute of Education, (4) Clinical Research Center，(5) Faculty of Medicine(1)

Research

Following studies have been extensively carried out in our laboratory with various biochemical, immunological,
molecular biological and statistical techniques:
1) Investigation of mechanism and development of new therapeutics for the treatment of rheumatoid arthritis
and dermato/polymyositis.
2) Establishment of evidence-based treatment of rheumatic diseases by implementing several cohort studies.
We collaborate with department of lifetime clinical immunology and pediatrics.
(2)

Education

We have provided medical students and graduates with the opportunity to obtain the ability to identify
important clinical problems and to solve them by clinical reasoning through their active participation into the― 285 ―

Bio-Environmental Response
Response
Bio-Environmental

diagnosis and management of various rheumatic diseases.
(3)

Clinical Services & Other Works

We have provided care to many patients with diverse rheumatic diseases. We have aimed to practice evidencebased medicine and to provide care that is in accordance with the global standard. We have contributed to thedevelopment of potential new drugs and treatments through participation into industry- as well as investigatorinitiated clinical trials for chemical and biological agents. We have also contributed to the reﬁnement of thecare of rheumatic disease patients through the conduct of various pharmacovigilance studies.
We cooperate with department of lifetime clinical immunology and pediatrics.
(4)

Publications[Original Articles]
1. Mineto Ota, Hideyuki Iwai, Kaori Imai, Mari Kamiya, Yoko Yoshihashi-Nakazato, Nobuyuki Miyasaka,
Hitoshi Kohsaka. Acute Tubulointerstitial Nephritis Associated with Inﬂiximab in a Patient with Crohn’s
Disease. Intern. Med.. 2016; 55(10); 1367-1370
2. Kohno S, Tamura K, Niki Y, Izumikawa K, Oka S, Ogawa K, Kadota J, Kamei K, Takata T, Takesue Y,
Teruya K, Tokimatsu I, Fukuda T, Maesaki S, Makimura K, Mikamo H, Mitsutake K, Miyazaki Y, Mori
M, Yasuoka A, Yano K, Yamanaka N, Yoshida M.. Executive summary of Japanese domestic guidelines
for management of deep-seated mycosis. Med Mycol J . 2016; 57(4); 117-163
3. Kato H, Mori M, Oba M, Kawahara H, Kaneko T.. Persistence and Half-lives of Anti-measles and Antirubella Antibodies in Japanese Hospital Workers: A Longitudinal Study. Intern Med. 2016; 55(18);2587-2594
4. Hirokazu Sasaki, Hayato Yamazaki, Hitoshi Kohsaka. A Case of ANCA-associated Large Vessel Vasculitis
with Multiple Saccular Aneurysms. J. Rheumatol.. 2016.01; 43(1); 179
5. Hiroyuki Tomimitsu, Akiko Ohta, Masaki Nagai, Motoko Nishina, Shoichiro Ishihara, Hitoshi Kohsaka.
Epidemiologic analysis of the clinical features of Japanese patients with polymyositis and dermatomyositis.
Mod Rheumatol. 2016.01; 26(3); 398-402
6. Tadashi Hosoya, Hideyuki Iwai, Yu Yamaguchi, Kimito Kawahata, Nobuyuki Miyasaka, Hitoshi Kohsaka.
Cell cycle regulation therapy combined with cytokine blockade enhances antiarthritic eﬀects without
increasing immune suppression. Ann. Rheum. Dis.. 2016.01; 75(1); 253-259
7. Matsuo Yusuke, Mizoguchi Fumitaka, Saito Tetsuya, Kawahata Kimito, Ueha Satoshi, Matsushima Kouji,
Inagaki Yutaka, Miyasaka Nobuyuki, Kohsaka Hitoshi. Local ﬁbroblast proliferation but not inﬂux is
responsible for synovial hyperplasia in a murine model of rheumatoid arthritis. Biochem Biophys Res
Commun. 2016.02; 470(3); 504-509
8. Yokoyama K, Mori M, Yoshida A. Mycophenolate mofetil therapy for two cases of antiphospholipid
antibody-associated chorea. Mod Rheumatol. 2016.02;
9. Aya Inaba, Yuko Hamasaki, Kenji Ishikura, Riku Hamada, Tomoyuki Sakai, Hiroshi Hataya, Fumiyo
Komaki, Tetsuji Kaneko, Masaaki Mori, Masataka Honda. Long-term outcome of idiopathic steroidresistant nephrotic syndrome in children. Pediatr. Nephrol.. 2016.03; 31(3); 425-434
10. Y Tagawa, T Saito, K Takada, K Kawahata, H Kohsaka. Successful treatment of severe refractory lupus
hepatitis with mycophenolate mofetil. Lupus. 2016.04; 25(5); 543-546
11. Akie Hirata, Yasuo Suenaga, Tomoya Miyamura, Toshihiro Matsui, Shigeto Tohma, Eiichi Suematsu,
Keizo Ohnaka, Ryoichi Takayanagi. Eﬀect of early treatment on physical function in daily managementof rheumatoid arthritis: a 5-year longitudinal study of rheumatoid arthritis patients in the National
Database of Rheumatic Diseases in Japan. Int J Rheum Dis. 2016.04;
12. Ge X, Tsang K, He L, Garcia RA, Ermann J, Mizoguchi F, Zhang M, Zhou B, Zhou B, Aliprantis AO.
NFAT restricts osteochondroma formation from entheseal progenitors JCI Insight. 2016.04; 1(4); e86254― 286 ―

Bio-Environmental
Bio-Environmental Response
Response

13. Fumio Hirano, Fumitaka Mizoguchi, Masayoshi Harigai, Nobuyuki Miyasaka, Hitoshi Kohsaka. Tacrolimus
successfully used to control refractory eosinophilic granulomatosis with polyangiitis complicated by invasive aspergillosis and chronic hepatitis B. International Journal of Rheumatic Diseases. 2016.04;
14. Mineto Ota, Mari Kihara, Akito Takamura, Hitoshi Kohsaka. Anti-EJ antibody positive interstitial lungdisease with skin changes at the ﬁngertips. Nihon Rinsho Meneki Gakkai Kaishi. 2016.05; 39(2); 150-153
15. Mari Kamiya, Peter Y Shane, Makoto Soejima, Shuji Tohda, Nobuyuki Miyasaka, Hitoshi Kohsaka.
IgG4-Related Sialoadenitis with a Skin Lesion and Multiple Mononeuropathies Suggesting Coexistent
Cryoglobulinemic Vasculitis. Intern. Med.. 2016.05; 55(10); 1355-1361
16. Tomo Nozawa, Masaaki Mori, Kenichi Nishimura, Nodoka Sakurai, Masako Kikuchi, Ryoki Hara, Shumpei
Yokota. Usefulness of two interferon-γ release assays for rheumatic disease. Pediatr Int. 2016.05; 58(5);347-352
17. Kawasaki Aya, Hasebe Narumi, Hidaka Misaki, Hirano Fumio, Sada Ken-Ei, Kobayashi Shigeto, Yamada Hidehiro, Furukawa Hiroshi, Yamagata Kunihiro, Sumida Takayuki, Miyasaka Nobuyuki, Tohma
Shigeto, Ozaki Shoichi, Matsuo Seiichi, Hashimoto Hiroshi, Makino Hirofumi, Arimura Yoshihiro, Harigai
Masayoshi, Tsuchiya Naoyuki. Protective Role of HLA-DRB1*13:02 against Microscopic Polyangiitis and
MPO-ANCA-Positive Vasculitides in a Japanese Population: A Case-Control Study. PLoS One. 2016.05;
11(5); e0154393
18. H Takada, T Hosoya, N Kimura, K Kawahata, H Kohsaka. Successful use of rituximab in a patient withrefractory thrombotic thrombocytopaenic purpura complicated by polymyositis. Scand. J. Rheumatol..
2016.06; 1-2
19. Yoko Yoshihashi-Nakazato, Kimito Kawahata, Naoki Kimura, Nobuyuki Miyasaka, Hitoshi Kohsaka.
Interferon-γ, but Not Interleukin-4, Suppresses Experimental Polymyositis. Arthritis & rheumatology(Hoboken, N.J.). 2016.06; 68(6); 1505-1510
20. Toru Doi, Tokuhide Doi, Naohiro Kawamura, Toshihiro Matsui, Akiko Komiya, Zaika Tei, Gaku Niitsuma,
Junichi Kunogi. The usefulness of neutrophil CD64 expression for diagnosing infection after orthopaedic
surgery in dialysis patients. J Orthop Sci. 2016.07; 21(4); 546-551
21. Sakai Ryoko, Hirano Fumio, Kihara Mari, Yokoyama Waka, Yamazaki Hayato, Harada Sayoko, Nanki
Toshihiro, Koike Ryuji, Miyasaka Nobuyuki, Harigai Masayoshi. High prevalence of cardiovascular comorbidities in patients with rheumatoid arthritis from a population-based cross-sectional study of a Japanese
health insurance database. Mod Rheumatol. 2016.07; 26(4); 522-528
22. Atsushi Hashimoto, Akiko Komiya, Toshihiro Matsui, Shigeto Tohma. Neutrophil CD64 for the diagnosisof organizing pneumonia in patients with rheumatoid arthritis. Mod Rheumatol. 2016.09; 26(5); 802-803
23. Taichi Kanetaka, Masaaki Mori, Ken-Ichi Nishimura, Tomo Nozawa, Masako Kikuchi, Nodoka Sakurai,
Ryoki Hara, Kazuko Yamazaki, Shumpei Yokota. Characteristics of FDG-PET ﬁndings in the diagnosisof systemic juvenile idiopathic arthritis. Mod Rheumatol. 2016.09; 26(3); 362-367
24. Hideaki Kato, Masaaki Mori, Mari Oba, Haruyo Kawahara, Takeshi Kaneko. Persistence and Half-livesof Anti-measles and Anti-rubella Antibodies in Japanese Hospital Workers: A Longitudinal Study. Intern
Med.. 2016.09; 55(18); 2587-2594
25. Nobuo Sakata, Sayuri Shimizu, Fumio Hirano, Kiyohide Fushimi. Epidemiological study of adult-onset
Still’s disease using a Japanese administrative database. Rheumatol. Int.. 2016.10; 36(10); 1399-1405
26. Takeshi Kusuda, Tadashi Hosoya, Takayasu Mori, Katsuhito Ihara, Hidenori Nishida, Motoko Chiga, Eisei
Sohara, Tatemitsu Rai, Ryuji Koike, Shinichi Uchida, Hitoshi Kohsaka. Acquired Gitelman Syndrome inan Anti-SSA Antibody-positive Patient with a SLC12A3 Heterozygous Mutation. Intern. Med.. 2016.11;55(21); 3201-3204
27. Yasuyoshi Ishiwata, Masashi Nagata, Takafumi Arai, Misato Makiishi, Maho Yoshikawa, Hiromitsu Takahashi, Hitoshi Kohsaka, Masato Yasuhara. Eﬀects of Miconazole Oral Gel on Blood Concentrations of
Tacrolimus and Cyclosporine: A Retrospective Observational Study. Ther Drug Monit. 2016.12; 38(6);717-721― 287 ―

Bio-Environmental Response
Response
Bio-Environmental

28. Fumio Hirano, Waka Yokoyama, Hayato Yamazaki, Koichi Amano, Atsushi Kawakami, Taichi Hayashi,
Naoto Tamura, Shinsuke Yasuda, Hiroaki Dobashi, Takao Fujii, Satoshi Ito, Yuko Kaneko, Toshihiro
Matsui, Yasuaki Okuda, Kazuyoshi Saito, Fumihito Suzuki, Ryusuke Yoshimi, Ryoko Sakai, Ryuji Koike,
Hitoshi Kohsaka, Nobuyuki Miyasaka, Masayoshi Harigai, . Achieving simpliﬁed disease activity index
remission in patients with active rheumatoid arthritis is associated with subsequent good functional andstructural outcomes in a real-world clinical setting under a treat-to-target strategy. Mod Rheumatol.
2016.12; 1-9
29. Okiyama N, Hasegawa H, Oida T, Hirata S, Yokozeki H, Fujimoto M, Miyasaka M, Kohsaka H. Experimental myositis inducible with transfer of dendritic cells presenting a skeletal muscle C protein-derived
CD8 epitope peptide Int Immunol.
30. Kimura N, Hirata S, Miyasaka N, Kawahata K, Kohsaka H. Injury and subsequent regeneration of muscles
activate local innate immunity to facilitate development and relapse of autoimmune myositis Arthritis
Rheumatol.
31. Eri Kato, Tetsuji Sawada, Koichiro Tahara, Haeru Hayashi, Mayu Tago, Hiroaki Mori, Jinju Nishino,
Toshihiro Matsui, Shigeto Tohma. The age at onset of rheumatoid arthritis is increasing in Japan: anationwide database study[Misc]
1. Shigeru Kohno, Kazuo Tamura, Yoshihito Niki, Koichi Izumikawa, Shinichi Oka, Kenji Ogawa, Junichi
Kadota, Katsuhiko Kamei, Yoshinobu Kanda, Tetsuya Kiuchi, Kazutoshi Shibuya, Syunji Takakura, Toru
Takata, Yoshio Takesue, Katsuji Teruya, Issei Tokimatsu, Takahiro Fukuda, Shigefumi Maesaki, Koichi
Makimura, Hiroshige Mikamo, Kotaro Mitsutake, Yoshitsugu Miyazaki, Masaaki Mori, Akira Yasuoka,
Keiko Yano, Noboru Yamanaka, Minoru Yoshida. Executive Summary of Japanese Domestic Guidelines
for Management of Deep-seated Mycosis 2014. Med Mycol J. 2016.04; 57(4); E117-E163[Conference Activities & Talks]
1. Tsuyoshi Yamada, Hideyuki Iwai, Sayuri Nitta, Masakazu Nagahori, Masayoshi Harigai. Creating Medical Innovators – a Whole-brain Approach to Hea. the 13th Asia Paciﬁc Medical Education Conference
2016.01.15 Singapore
2. Fumitaka Mizoguchi, Kamil Slowikowski, Nir Hacohen, Peter A Nigrovic, Soumya Raychaudhuri, Michael
B Brenner. Identiﬁcation of Synovial Fibroblast Subsets That Deﬁne Pathology in Rheumatoid Arthritis.
Stromal cells in immunity, Keystone symposia 2016.02.09 Keystone, USA
3. Fumitaka Mizoguchi. Single cell and whole-tissue transcriptomics identify pathogenic ﬁbroblast subsets
in rheumatoid arthritis. Queen Elizabeth Hospital, University of Birmingham, UK 2016.03.03 Queen
Elizabeth Hospital, University of Birmingham, UK
4. Tetuya Saito,Kimito Kawahata,Hitoshi Kohsaka. Pathogenic role of lysol oxidase-like 2 in rheumatoid
arthritis. The 60th Annual General Assembly and Scientiﬁc Mwwting of the Japan College of Rheumatology 2016.04 Yokohama
5. Hitoshi Kohsaka. Use of anti-myositis biological agents available and unavailable on the market. 2016
Annual Symposium in Rheumatism Research Institute 2016.06.18 Seoul, Korea
6. Fumitaka Mizoguchi, Kamil Slowikowski, Soumya Raychaudhuri, Michael B Brenner. . Single cell transcriptomics and protein expression analysis deﬁne disease-speciﬁc ﬁbroblast subsets in rheumatoid arthritis
joint tissue.. Second Osteoimmunology confernece 2016.07.06 Okinawa
7. Hirokazu Sasaki, Akito Takamura, Kimito Kawahata, Kohsuke Imai, Tomohiro Morio, and Hitoshi Kohsaka.
Altered peripheral lymphocyte subset repertoires that reﬂect clinical features of dermatomyositis andpolymyositis. The 13th International Workshop on Autoantibodies and Autoimmunity 2016.10.11 Kyoto
8. Hirokazu Sasaki, Akito Takamura, Kimito Kawahata, Kohsuke Imai, Tomohiro Morio, and Hitoshi Kohsaka.
Abnormal composition of circulating T and B cells in patients with polymyositis and dermatomyositis is
more biased in those with interstitial lung diseases.. ACR/ARHP Annual Meeting 2016.11.13 Washington,DC― 288 ―

Bio-Environmental
Bio-Environmental Response
Response

9. Fumitaka Mizoguchi, Hisanori Hasegawa, and Hitoshi Kohsaka. Functional Screening of MicroRNAs Using
the Inhibitor Library Identiﬁed MicroRNAs to Regulate Expression of MMP-3 and IL-6 in Rheumatoid
Arthritis Synovial Fibroblasts. 2016 American College of Rheumatology/ Association of Rheumatology
Health Professionals Annual Meeting 2016.11.14 Washington DC, USA― 289 ―

Bio-Environmental Response

Bio-Environmental Response

Dermatology

Professor: Hiroo YOKOZEKI
Associate Professor: Ken IGAWA
Junior Associate Professor: Takeshi NAMIKI,Takaaki HANAFUSA
Project Junior Associate Professor: Kaoru TAKAYAMA
Assistant Professor: Shoun TOKORO,Takashi HASHIMOTO,Kohei NOJIMA
Senior Resident: Sakiko CHIKAZAWA,Ai INAGE,Yumiko SONE,Keiko INUI,Michiko NAKAMURA
Resident: Chika OMIGAWA
Doctoral Student: Rie YU, Takichi MUNETSUGU,Minako INAZAWA,Chen YUE,Sayaka KIKUCHI,
Aiko FURUI, Kohei KATO, Sally ESHIBA, Rumi SUZUKI, Takahiro ISHIKAWA,
Al-Busani Hind Abdullah Ahmed, Yutaro HANDATechnical Assistant: Chiyako MIYAGISHI,Yoshiko ITO
Staﬀ Assistant: Masae SAKATA,Mayuko HAYASHI,Yu KAWAMURA(1)

OutlineDermatology is a department of medical science which educates students to make a diagnosis and treatment forskin diseases. Main objective of Dermatology in the graduate course is to provide students opportunity to study
advanced Immunodermatology, physiology, pathology and allergology, and also to study making diagnosis ofskin diseases and operation techniques. Students are also taught on skin oncology (melanoma, angiosarcoma)
and its related laboratory technology depending on their research project.
(2)

Research

1) Mechanisms of contact hypersensitivity2) Pathological etiology of atopic dermatitis
3) Mechanisms of eosinophil recruitment to the skin4) Roles of basophils in human skin diseases
5) Functional roles of PGD2 and its receptors in allergic inﬂammation
6) Therapeutic approach for skin diseases by stable form of galectin-97) Analysis of pathological mochanisms’ of hyperhidrosis8) Investigation of mediators for itch
9) Pathological etiology of chronic prurigo
10) Therapeutic approach for angiosarcoma with HVJ-E.
11) To establish the in vitro diseases model of dermatological disorders using human induced pluripotent stemcell12) Murine food allergy model with transcutaneous sensitization(3)

Clinical Performances

Dermatology clinic provides an advanced treatment for skin diseases; skin tumors, infectious diseases, skinallergy, collagen diseases and psoriasis. Recently, we established the gene thearies (STAT6 decoy ODN) for
severe atopic dermatitis in the clinic.

― 290 ―

Bio-Environmental Response

Bio-Environmental Response(4)

Publications[Original Articles]
1. Yokozeki H.. New Pathologies of Skin Disorders Identiﬁed from the History of Perspiration Research.
Curr Probl Dermatol.. 2016; 51; 1-6
2. Suzuki R, Yikelamu A, Tanaka R, Igawa K, Yokozeki H, Yaguchi T.. Studies in Phylogeny, Development of
Rapid IdentiﬁcationMethods, Antifungal Susceptibility, and Growth Rates of Clinical Strains of Sporothrix
schenckii Complex in Japan. Med Mycol J.. 2016; 57(3); e47-e57
3. Sone Y, Namiki T, Munetsugu T, Ueno M, Tokoro S, Nishizawa A, Takayama K, Yokozeki H. A case ofsubungual melanoma with bone invasion: Destructive local invasion and multiple skin metastases. J Eur
Acad Dermatol Venereol. 2016.01; 30(6); 1049-1050
4. Yoshioka Y, Hanafusa T, Namiki T, Nojima K, Amano M, Tokoro S, Igawa K, Yokozeki H. . Drug-induced
hypersensitivity syndrome by ethambutol: A case report. J Dermatol.. 2016.02; 43(8); 971-972
5. Kondo M, Igawa K, Munetsugu T, Shibama S, Nishizawa A, Tokoro S, Yokozeki H.. Increasing Numbersof Mast Cells in Skin Lesions of Hyperpigmented Mycosis Fungoides with Large-Cell Transformation. Ann
Dermatol.. 2016.02; 28(1); 115-116
6. Hirohata A, Hanafusa T, Igawa K, Inoue-Nishimoto T, Mabuchi-Kiyohara E, Nishide M, Yokozeki H,
Ikegami R.. Oral tacrolimus for the treatment of generalized morphea. Eur J Dermatol. . 2016.02; 26(1);112-113
7. Arima Y, Namiki T, Kato K, Tokoro S, Takayama K, Yokozeki H.. Case of extensive pyoderma gangrenosum with a neutrophilic leukemoid reaction. J Dermatol.. 2016.02; 43(2); 217-218
8. Matsuura-Otsuki Y, Hanafusa T, Igawa K, Sato H, Nishizawa A, Yokozeki H.. Macrophage activation
syndrome triggered by disseminated tuberculosis with tuberculous gumma in a patient with adult-onset
Still’s disease and Good’s syndrome. Eur J Dermatol.. 2016.03; 26(3); 309-311
9. Namiki T, Miura K, Ueno M, Tanaka K, Yokozeki H.. Case of basal cell carcinoma with myoepithelial
diﬀerentiation: Its characteristic clinical and histopathological features. J Dermatol.. 2016.03; 43(9);1109-1110
10. Namiki T, Arai M, Miura K, Yokozeki H.. A case of phakomatosis pigmentovascularis type II: portwine stain and dermal melanocytosis with cutis marmorata telangiectatica congenita-like lesions. Eur J
Dermatol.. 2016.03; 26(3); 203-303
11. Hanafusa T, Kato K, Azukizawa H, Miyazaki J, Takeda J, Katayama I.. B-1 B cell progenitors transiently
and partially express keratin 5 during diﬀerentiation in bone marrow. J Dermatol Sci.. 2016.03; 81(3);173-181
12. Suzuki R, Fukuyama K, Miyazaki Y, Namiki T. Contact urricaria syndrome and protein contact dermatitis
caused by glycerin enema. JAAD Case Reports . 2016.03; 2(2); 108-110
13. Hirohata A, Hanafusa T, Igawa K, Inoue-Nishimoto T, Mabuchi-Kiyohara E, Nakai C, Kasugai T, Yokozeki
H, Ikegami R.. Bullous erythema ab igne with cutaneous reactive angiomatosis. Eur J Dermatol. .
2016.04; 26(2); 191-192
14. Inui K, Hanafusa T, Namiki T, Ueno M, Igawa K, Yokozeki H.. Intractable Postoperative Wounds Caused
by Self-Inﬂicted Trauma in a Patient with Cutaneous Munchausen Syndrome Presenting as a Pyoderma
Gangrenosum-Like Lesion. Case Rep Dermatol.. 2016.04; 8(1); 97-101
15. Namiki T, Miura K, Ueno M, Arima Y, Nishizawa A, Yokozeki H.. Four Diﬀerent Tumors Arising in a
Nevus Sebaceous. Case Rep Dermatol.. 2016.04; 8(1); 75-79
16. Matsuura-Otsuki Y, Hanafusa T, Namiki T, Ueno M, Inazawa M, Tokoro S, Igawa K, Nakamura M, Igari
K, Inoue Y, Yokozeki H.. Peripheral arterial bypass surgery for intractable wounds caused by limited
cutaneous systemic sclerosis. J Dermatol. . 2016.04;
17. UgajinT,ShibamaS,NishidaK,YokozekiH..Metallothioneinsarerequiredforhumanbasophilinterleukin-4geneinductionviaFcɛRI stimulation.AllergolInt..2016.04;― 291 ―

Bio-Environmental Response

Bio-Environmental Response

18. Nojima K, Namiki T, Inui K, Hanafusa T, Miura K, Asakage T, Yokozeki H.. Rapid reduction of nasal
melanoma by nivolumab. Eur J Dermatol.. 2016.04; 26(4); 405-406
19. Namiki T, Ichiyama S, Funasaka Y, Ito M, Kanzaki A, Miura K, Nojima K, Saeki H, Yokozeki H, Tanaka
M. . Dermoscopy of pigmented papillated Bowen disease: A report of two cases. J Dermatol.. 2016.05;
20. Namiki T, Miura K, Nojima K, Ueno M, Tanaka K, Tanaka M, Yokozeki H. . Ulcerated giant pilomatricoma with appearance of cutaneous malignancy on positron emission tomography/computed tomography.
J Dermatol. . 2016.05;
21. Kato K, Namiki T, Tokoro S, Miura K, Yokozeki H.. Histiocytoid Sweet syndrome with ophthalmologic
involvements: A novel association with uveitis. J Dermatol.. 2016.05;
22. Amano M, Hanafusa T, Inui K, Nakadai T, Nojima K, Tokoro S, Namiki T, Igawa K, Miura K, Yokozeki
H.. Microscopic polyangiitis presenting with multiple punched-out ulcers: A case report. J Dermatol. .
2016.05;
23. Namiki T, Takahashi M, Nojima K, Ueno M, Hanafusa T, Tokoro S, Yokozeki H.. Phakomatosis pigmentovascularis type IIb: A case with Klippel-Trenáunay syndrome and extensive dermal melanocytosis as
nevus of Ota, nevus of Ito and ectopic Mongolian spots. J Dermatol.. 2016.07;
24. Namiki T, Iikawa M, Otsuki Y, Ueno M, Yokozeki H.. Ectopic extramammary Paget disease mimicking
Bowen disease. J Am Acad Dermatol. . 2016.07; 75(1); e9-e10
25. Kato K, Namiki T, Tokoro S, Takayama K, Yokozeki H. . Bullous eosinophilic annular erythema. J
Dermatol.. 2016.08;
26. Funazumi M, Namiki T, Arima Y, Kato K, Nojima K, Tanaka K, Miura K, Yokozeki H.. Increased
Inﬁltration of CD8(+) T Cells by Dacarbazine in a Patient with Mucosal Penile Melanoma Refractory to
Nivolumab. Ann Dermatol. . 2016.08; 28(4); 486-490
27. Chen Y, Yokozeki H, Katagiri K. . Physiological and functional changes in the stratum corneum restoredby estrogen in an ovariectomized mice model of climacterium. Exp Dermatol.. 2016.09;
28. Munetsugu T, Igawa K, Fujimoto T, Shibama S, Nishizawa A, Yokozeki H.. Cold-induced hyperhidrosis:
possible association with hyper-IgE syndrome. Int J Dermatol.. 2016.10;
29. Kato K, Namiki T, Yokozeki H.. Acquired anhidrosis in a case of autoimmune autonomic ganglionopathy.
J Dermatol.. 2016.10;
30. Munetsugu T, Fujimoto T, Oshima Y, Sano K, Murota H, Satoh T, Iwase S, Asahina M, Nakazato
Y, Yokozeki H.. Revised guideline for the diagnosis and treatment of acquired idiopathic generalized
anhidrosis in Japan. J Dermatol.. 2016.10;
31. Chikazawa S, Hanafusa T, Ozaki K, Namiki T, Amano M, Ueno M, Tokoro S, Igawa K, Sato T, Ishikawa
K, Yokota T, Yokozeki H.. Incomplete Behçet’s disease with calf muscle pain and MRI hyperintensity due
to possible thrombophlebitis. Eur J Dermatol.. 2016.10;
32. Nojima K, Namiki T, Tokoro S, Miura K, Yokozeki H.. A case of nodular subungual melanoma without
Hutchinson’s nail sign. Australas J Dermatol.. 2016.10;
33. Matsuura-Otsuki Y, Hanafusa T, Namiki T, Ueno M, Tokoro S, Igawa K, Yokozeki H.. Relapsed nasal
extranodal NK/T-cell lymphoma with secondary cutaneous involvement presenting as a phlegmon-likelesion. Eur J Dermatol.. 2016.10; 26(6); 630-631
34. Namiki T, Miura K, Tokoro S, Tanaka M, Yokozeki H.. Dermoscopic features of lymphocytoma cutis: A
case report of a representative dermoscopic feature. Dermatol. . 2016.11; 43(11); 1367-1368
35. Kato K, Igawa K, Nishizawa A, Takayama K, Yokozeki H. . Allergic contact dermatitis induced by theanionic surfactant, sodium N-methyl-N-(1-oxododecyl)-beta-alaninate, contained in a daily-use shampoo.
J Eur Acad Dermatol Venereol.. 2016.11; 30(11); e123-e124
36. Amano M, Hanafusa T, Chikazawa S, Ueno M, Namiki T, Igawa K, Miura K, Yokozeki H.. Bazex
Syndrome in Lung Squamous Cell Carcinoma: High Expression of Epidermal Growth Factor Receptor in
Lesional Keratinocytes with Th2 Immune Shift. Case Rep Dermatol.. 2016.12; 8(3); 358-362― 292 ―

Bio-Environmental Response

Bio-Environmental Response

37. Namiki T, Tokoro S, Hanafusa T, Yokozeki H.. Image Gallery: Periorbital and temporal dermal melanocytosis of hypohidrotic ectodermal dysplasia. Br J Dermatol.. 2016.12; 175(6); e146-e147
38. Yu R, Igawa K, Handa Y, Munetsugu T, Satoh T, Yokozeki H.. Basophils and mast cells are crucial forreactions due to epicutaneous sensitization to ovalbumin. Exp Dermatol.. 2016.12;
39. Namiki T, Hanafusa T, Ueno M, Miura K, Yokozeki H.. Severe Oral Ulcers Associated With Nivolumab
Treatment. JAMA Dermatol. . 2016.12;[Books etc]
1. Yokozeki H, Murota H, Katayama I.. Current Problems in Dermatology. Perspiration Research.. Karger„
2016
2. Yokozeki H.. Current Problems in Dermatology. New pathologies of skin disorders identiﬁed from thehistory of perspiration research. Perspiration Research.. Karger„ 2016
3. Inoue R. . Current Problems in Dermatology. New ﬁnding on the mechanism of perspiration including
aquaporin-5 water channel. . Karger„ 2016
4. Nishizawa A.. Current Problems in Dermatology. Dyshidrotic Eczema and its Relationship to metalallergy.. Karger„ 2016
5. Fujimoto T.. Current Problems in Dermatology. Pathophysiology and treatment of Hyperhidrosis..
Karger„ 2016
[Conference Activities & Talks]
1. Namiki T.. Overexpression of NUAK2 has a signiﬁcant impact on the survival of acral melanoma patients.
. 46th Annual ESDR Meeting 2016 2016.09
2. Hanafusa T.. Evaluation of endothelial cells derived from induced pluripotent stem cells from systemicsclerosis patients .. 46th Annual ESDR Meeting 2016 2016.09
3. Hashimoto T.. Implication of amphiregulin in basophil-dependent prurigo-like skin reactions.. 46th Annual ESDR Meeting 2016.09
4. Namiki T, Nojima K, Funazami M, Ishikawa M, Kaneko Y, Tanemura A, Katayama I, Mori T, Yamazaki
N, Yokozeki H, Hearing VJ. Over-expression of NUAK2 has a signiﬁcant impact on the survival of acralmelanomas patients and a relevance with DNA copy number gain. The 27th Annual Meeting of theJapanese Society for Pigment Cell Research 2016.10
5. Hanafusa T, Namiki T, Amano M, Inui K, Chikazawa S, Nojima K, Ueno M, Igawa K, Yokozeki H.
. Two cases of adverse reactions with anti-PD-1 and anti-CTLA4 antibody therapies used for treating
malignant melanoma manifesting as graft-versus-host-like reactions and autommune hypophysitis.. The
12th Meeting of the German-Japanese Society of Dermatology 2016.10
6. Hashimoto T, Satoh T, Yokozeki H. . Deneralized pruritus in primary sclerosing cholangitis: implicationsof histamine release by lysophosphatidic acid.. The 12th Meeting of the German-Japanese Society of
Dermatology 2016.10
7. Hashimoto T, Satoh T, Yokozeki H.. Pruritus in scabies infestation: Implications of Th2 immunity andIL-31.. Montagna Symposium on Biology of Skin 2016.10
8. Chen Y, Yokozeki H, Katagiri K. . Dysfuntion of the corneum in an ovariectomized mice model ofclimacterium is associated with aberrant epidermal diﬀerentiation and decreased expression of desmoglein1.. The 12th Meeting of the German-Japanese Society of Dermatology 2016.10
9. Funazumi M, Nojima K. Ishikawa M. Kaneko Y, Tanemura A, Katayama I, Mori T, Yamazaki N, Yokozeki
H, Hearling V.J.. The expression of NUAK2 aﬀects the prognosis of acral melanoma patiants. . The 27th
Annual Meeting of the Japanese Society For Pigment Cell Research,Gifu 2016.11
10. Omigawa C, Chikazawa S, Hashimoto T, Hanafusa T, Namiki T, Igawa K, Yokozeki H.. Multiple ﬁxed
drug eruption caused by trimethoprim/sufamethoxazole in a patient with systemic lupus erythematosus
and anti-laminin 332 mucous membranous pemphigoid,. The 4th Eastern Asia Dematology 2016.11― 293 ―

Bio-Environmental Response

Bio-Environmental Response

11. Namiki T, Nojima K, Funazumi M, Ishikawa M, Kaneko Y, Tanemura A, Katayama I, Mori T, Yamazaki
N, Yokozeki H, Hearing VJ. The signiﬁcance of over-expression of NUAK2 on the survival of patientswith acral melanomas and its relevance with DNA copy number gain. The 41th Annual Meeting of theJapanese Society for Investigative Dermatology 2016.12― 294 ―

Bio-Environmental Response

NCCHDChildHealthandDevelopment
1. Stuffs and Students
Collaborative Professor
Collaborative Professor
Collaborative Professor
Collaborative Professor
Collaborative Professor
Collaborative Professor

Akutsu, Hidenori
Onodera, Masashi
Fukami, Maki
Hata, Kenichiro
Takada, Shuji
Matsumoto, Kenji
2. Purpose of Education
The goal of this course is to learn the developmental process of human life from the viewpoints of latest molecular biology andgenetics. Medical science for child health and development is the study to comprehensively grasp various health problems related
to “human life cycle” to begin with the fertilization and to continue to the next generation through generation and development.
Students of this course are required to understand a role and a function of medical care for child health and development, to acquireability to handle such health problems and support relevant person with specialized theory and technique.


3. Research Subjects
1)Exploring molecular mechanism for acquisition of zygote totipotency, epigenetic reprogramming and pluripotency in stem cells
Application studies for reproductive medicine and regenerative medicine(Akutsu, Hidenori; Center for Regenerative Medicine, National Institute for Child Health and Development)
Exploring molecular mechanism for acquisition of zygote totipotency, epigenetic reprogramming and pluripotency in stem cells.
Application studies for reproductive medicine and regenerative medicine.
2) Studying for cellular model in human severe disease by advancing flow cytometry(Onodera, Masashi; Dept. of Human Genetics, National Institute for Child Health and Development)
We aim to identify causative genes for child intractable hereditary diseases and analyze their functions to develop new genebased therapeutic options. We also establish iPS cells from peripheral blood or skin fibroblasts obtained from patients withintractable hereditary diseases such as primary immunodeficiencies and congenital metabolic disorders.
3) Elucidation of genetic abnormality in congenital severe metabolic diseases using advanced genetic analysis(Fukami, Maki; Dept. of Molecular Endocrinology, National Institute for Child Health and Development)
Our objective is to clarify the molecular basis of congenital endocrine-related disorders and apply our findings to new
innovations in clinical medicine. We investigate the molecular basis of single gene disorders, epigenetic/inprinting disorders, andmultifanctorial disorder.
4) Elucidating for molecular mechanism of perinatal abnormality using system biology(Hata, Kenichiro; Dept. of Maternal-Fetal Biology, National Institute for Child Health and Development)
We aim to clarify mechanisms underlysing abnormalities in fetal development and placentation, and/or perinatal diseases with
developmental defects. To identify the underlying mechanisms of perinatal diseases, we take advantage of post-genomic
technologies and investigate etiologies using an integrated genomic and epigenomic approach.
5)Identification of target molecules in severe diseases and establishment of disease model mice by studying molecularmechanisms of genomic imprinting, gametogenesis and sexual differentiation(Takada, Shuji; Dept. of Systems Biomedicine, National Institute for Child Health and Development)
Our aim is to reveal the molecular mechanisms underlying embryonic development, cell differentiation and tissue formation andapply our findings to understand the causes of developmental diseases.
6)Elucidation for allergic disease mechanism and target molecules using molecular biology and ‘omics’ technology(Matsumoto, Kenji; Dept. of Allergy and Clinical Immunology, National Institute for Child Health and Development)
Our mission is to clarify the precise pathogenic mechanisms of various immunological and allergic diseases, such as Kawasaki
disease, various allergic diseases, allogenic immune tolerance, congenital viral infections and severe infectious diseases. To
achieve this, we employ various experimental approaches, including epidemiology, clinical and basic research. Our ultimate aim is
to develop better means of preventing, diagnosing and treating allergic and immunological and infectious diseases based on our
research findings and cumulative knowledge.
― 295 ―




Bio-Environmental Response

4. Publications[Original Articles]

1. Fukuda A, Mitani A, Miyashita T, Sado T, Umezawa A, Akutsu H*. Maintenance of Xist imprinting depends
on chromatin condensation state and Rnf12 dosage in mice. PLoS Genet 2016; 12(10): e1006375.
2. Akutsu H, Nasu M, Morinaga S, Motoyama T, Homma N, Machida M, Yamazaki-Inoue M, Okamura K,
Nakabayashi K, Takada S, Nakamura N, Kanzaki S, Hata K, Umezawa A. In vivo maturation of human embryonicstem cell-derived teratoma over time. Regenerative Therapy 2016; 5: 31-39.
3. Liao H, Sato H, Chiba R, Kawai T, Nakabayashi K, Hata K, Akutsu H, Fujiwara S, Nakamura H. Humancytomegalovirus downregulates SLITRK6 expression through IE2. J Neurovirol. 2016 Aug 16. [Epub ahead ofprint]
4. Fukuda A, Mitani A, Miyashita T, Kobayashi H, Umezawa A, Akutsu H*. Spatiotemporal dynamics of Oct4
protein localization during preimplantation development in mice. Reproduction. 2016 Nov; 152(5): 417-430.
5. Tateno H, Saito S, Hiemori K, Kiyoi K, Hasehira K, Toyoda M, Onuma Y, Ito Y, Akutsu H, Hirabayashi J. Į2-6
sialylation is a marker of the differentiation potential of human mesenchymal stem cells. Glycobiology. 2016 Apr
2. pii: cww039. [Epub ahead of print]
6. Katoh-Fukui Y, Igarashi M, Nagasaki K, Horikawa R, Nagai T, Tsuchiya T, Suzuki E, Miyado M, Hata K,
Nakabayashi K, Hayashi K, Matsubara Y, Baba T, Morohashi K, Igarashi A, Ogata T, Takada S, Fukami M.
Testicular Dysgenesis/Regression without Campomelic Dysplasia in Patients Carrying Missense Mutations andUpstream Deletion of SOX9. Mol Genet Genom Med. 3(6): 550–557, 2016
7. Matsubara K, Murakami N, Fukami M, Kagami M, Nagai T, Ogata T. Risk assessment of medically assisted
reproduction and advanced maternal ages in the development of Prader-Willi syndrome due to UPD(15)mat. Clin
Genet. 89(5): 614–619, 2016
8. Kon M, Saito K, Mitsui T, Miyado M, Igarashi M, Moriya K, Nonomura K, Shinohara N, Ogata T, Fukami M.
Copy-Number Variations of the Azoospermia Factor Region or SRY Are Not Associated with the Risk of
Hypospadias. Sex Dev. 10(1): 12–15, 20169.
Maruo Y, Nagasaki K, Matsui K, Mimura Y, Mori A, Fukami M, Takeuchi Y. Natural course of congenital
hypothyroidism by dual oxidase 2 mutations from the neonatal period through puberty. Eur J Endocrinol. 174(4):453–463, 201610. Saito K, Matsuzaki T, Iwasa T, Miyado M, Saito H, Hasegawa T, Homma K, Inoue E, Miyashiro Y, Kubota T,
Irahara M, Ogata T, Fukami M. Steroidogenic pathways involved in androgen biosynthesis in eumenorrheic
women and patients with polycystic ovary syndrome. J Steroid Biochem Mol Biol. 158: 31–37, 201611. Shima H, Tanaka T, Kamimaki T, Dateki S, Muroya K, Horikawa R, Kanno J, Adachi M, Naiki Y, Tanaka H,
Mabe H, Yagasaki H, Kure S, Matsubara Y, Tajima T, Kashimada K, Ishii T, Asakura Y, Fujiwara I, Soneda S,
Nagasaki K, Hamajima T, Kanzaki S, Jinno T, Ogata T, Fukami M; Japanese SHOX study group. Systematic
Molecular Analyses of SHOX in Japanese Patients with Idiopathic Short Stature and Leri-Weill
Dyschondrosteosis. J Hum Genet. 61(7): 585–591, 2016
12. Fujisawa Y, Sakaguchi K, Ono H, Yamaguchi R, Kato F, Kagami M, Fukami M, Ogata T.㻌 Combined
Steroidogenic Characters of Fetal Adrenal and Leydig Cells in Childhood Adrenocortical Carcinoma. J Steroid
Biochem Mol Biol. 159: 86䇵93, 2016
13. Okuno M, Yorifuji T, Kagami M, Ayabe T, Urakami T, Kawamura T, Kikuchi N, Yokota I, Kikuchi T,
Amemiya S, Suzuki J, Ogata T, Sugihara S, Fukami M; Japanese Study Group of Insulin Therapy for Childhoodand Adolescent Diabetes (JSGIT). Chromosome 6q24 methylation defects are uncommon in childhood-onset nonautoimmune diabetes mellitus patients born appropriate- or large-for-gestational age. Clin Pediatr Endocrinol.
25(3): 99–102, 201614. Saito K, Matsuzaki T, Iwasa T, Miyado M, Saito H, Kubota T, Irahara M, Ogata T, Fukami M. Blood
allopregnanolone in patients with polycystic ovary syndrome. Clin Endocrinol. 85(1): 151–152, 201615. Sano S, Matsubara K, Nagasaki K, Kikuchi T, Nakabayashi K, Hata K, Fukami M, Kagami M, Ogata T.
Beckwith-Wiedemann syndrome and pseudohypoparathyroidism type Ib in a patient with multilocus methylation
defects: a female-dominant phenomenon. J Hum Genet. 61(8): 765–769, 2016
16. Yokoi T, Nishina S, Fukami M, Ogata T, Hosono K, Hotta Y, Azuma N. Genotype-phenotype correlation of
PAX6 gene mutations in aniridia. Hum Genome Var. 3: 15052, 2016
17. Koyama Y, Homma K, Fukami M, Miwa M, Ikeda K, Ogata T, Murata M, Hasegawa T. Classic and nonclassic 21-hydroxylase deficiency can be discriminated from P450 oxidoreductase deficiency in Japanese infants
by urinary steroid metabolites. Clin Pediatr Endocrinol. 25(2): 37–44, 201618. Narumi S, Amano N, Ishii T, Katsumata N, Muroya K, Adachi M, Toyoshima K, Tanaka Y, Fukuzawa R,
Miyako K, Kinjo S, Ohga S, Ihara K, Inoue H, Kinjo T, Hara T, Kohno M, Yamada S, Urano H, Kitagawa Y,
Tsugawa K, Higa A, Miyawaki M, Okutani T, Kizaki Z, Hamada H, Kihara M, Shiga K, Yamaguchi T, Kenmochi
M, Kitajima H, Fukami M, Shimizu A, Kudoh J, Shibata S, Okano H, Miyake N, Matsumoto N, Hasegawa T.
SAMD9 mutations cause a novel multisystem disorder, MIRAGE syndrome, and are associated with loss ofchromosome 7. Nat Genet. 48(7): 792–797, 2016
― 296 ―

Bio-Environmental Response

19. Nakamura A, Hamaguchi E, Horikawa R, Nishimura Y, Matsubara K, Sano S, Nagasaki K, Matsubara Y,
Umezawa A, Tajima T, Ogata T, Kagami M, Okamura K, Fukami M. Complex Genomic Rearrangement within the
GNAS Region Associated with Familial Pseudohypoparathyroidism Type 1b. J Clin Endocrinol Metab. 101(7):2623–2627, 2016
20. Naiki Y, Miyado M, Horikawa R, Katsumata N, Onodera M, Pang S, Ogata T, Fukami M. Extra-Adrenal
Induction of Cyp21a1 Ameliorates Systemic Steroid Metabolism in a Mouse Model of Congenital Adrenal
Hyperplasia. Endocr J. 63(10): 897–904, 2016
21. Ayabe T, Fukami M, Ogata T, Kawamura T, Urakami T, Kikuchi N, Yokota I, Ihara K, Takemoto K, Mukai T,
Nishii A, Kikuchi T, Mori T, Shimura N, Sasaki G, Kizu R, Takubo N, Soneda S, Fujisawa T, Takaya R, Kizaki Z,
Kanzaki S, Hanaki K, Matsuura N, Kasahara Y, Kosaka K, Takahashi T, Minamitani K, Matsuo S, Mochizuki H,
Kobayashi K, Koike A, Horikawa R, Teno S, Tsubouchi K, Mochizuki T, Igarashi Y, Amemiya S, Sugihara S.
Variants associated with autoimmune Type 1 diabetes in Japanese children: implications for age-specific effects ofcis-regulatory haplotypes at 17q12-q21. Diabet Med. 33(12): 1717–1722, 2016
22. Fujisawa Y, Fukami M, Hasegawa T, Uematsu A, Muroya K, Ogata T. Long-term clinical course in threepatients with MAMLD1 mutations. Endocr J. 63(9): 835–839, 2016
23. Goto M, Yamamoto Y, Ishii M, Nakamura A, Sano S, Kagami M, Fukami M, Saito R, Araki S, Kubo K,
Kawagoe R, Kawada Y, Kusuhara K. Sporadic pseudohypoparathyroidism type-1b with asymptomatic
hypocalcemia.. Pediatr Int. 58(11): 1229–1231, 201624. Shima H, Yatsuga S, Nakamura A, Sano S, Sasaki T, Katsumata N, Suzuki E, Hata K, Nakabayashi K,
Momozawa Y, Kubo M, Okamura K, Kure S, Matsubara Y, Ogata T, Narumi S, Fukami M. NR0B1 Frameshift
Mutation in a Boy with Idiopathic Central Precocious Puberty. Sex Dev. 10(4): 205–209, 201625. Suzuki E, Shima H, Toki M, Narumi S. Ogata T. Kamimaki T, Fukami M. Complex X-chromosomal
rearrangements in two women with ovarian dysfunction: Implications for chromothripsis/chromoanasynthesisdependent and -independent origins of complex genomic alterations. Cytogenet Genome Res. 150(2): 86-92, 2016
26. Okuno M, Kasahara Y, Onodera M, Takubo N, Okajima M, Suga S, Watanabe N, Suzuki J, Ayabe T, Urakami
T, Kawamura T, Kikuchi N, Yokota I, Kikuchi T, Amemiya S, Nakabayashi K, Hayashi K, Hata K, Matsubara Y,
Ogata T, Fukami M, Sugihara S. Nucleotide substitutions in CD101, the human homolog of a diabetes
susceptibility gene in non-obese diabetic mouse, in patients with type 1 diabetes. J Diabetes Investig. 2016. [Epubahead of print]
27. Miyado M, Inui M, Igarashi M, Katoh-Fukui Y, Takasawa K, Hakoda A, Kanno J, Kashimada K, Miyado K,
Tamano M, Ogata T, Takada S, Fukami M. The p.R92W variant of NR5A1/Nr5a1 induces testicular developmentof 46,XX gonads in humans, but not in mice: Phenotypic comparison of human patients and mutation-induced
mice. Biol Sex Differ. 7: 56, 2016
28. Miyado M, Miyado K, Nakamura A, Fukami M, Yamada G, Oda SI. Expression patterns of Fgf8 and Shh inthe developing external genitalia of Suncus murinus. Reproduction. 2016 [Epub ahead of print]
29. Montalbano A, Juergensen L, Roeth R, Weiss B, Fukami M, Fricke-Otto S, Binder G, Ogata T, Decker E,
Nuernberg G, Hassel D, Rappold GA. Retinoic acid catabolizing enzyme CYP26C1 is a genetic modifier in
SHOX deficiency. EMBO Mol Med. 8(12): 1455–1469, 2016
30. Naiki Y, Miyado M, Horikawa R, Katsumata N, Onodera M, Pang S, Ogata T, Fukami M. Extra-adrenal
induction of Cyp21a1 ameliorates systemic steroid metabolism in a mouse model of congenital adrenal
hyperplasia. Endocr J 63: 897-904, 2016.
31. Nagaya M, Watanabe M, Kobayashi M, Nakano K, Arai Y, Asano Y, Takeishi T, Umeki I, Fukuda T, Yashima
S, Takayanagi S, Watanabe N, Onodera M, Matsunari H, Umeyama K, Nagashima H. A transgenic-cloned pig
model expressing non-fluorescent modified Plum. J Reprod Dev 62: 511-520, 2016.
32. Kawano Y, Nakae J, Watanabe N, Kikuchi T, Tateya S, Tamori Y, Kaneko M, Abe T, Onodera M, Itoh H.
Colonic Pro-inflammatory Macrophages Cause Insulin Resistance in an Intestinal Ccl2/Ccr2-Dependent Manner.
Cell Metab 24: 295-310, 2016.
33. Ichida Y, Utsunomiya Y, Onodera M: Effect of the linkers between the zinc fingers in zinc finger protein 809
on gene silencing and nuclear localization. BBRC 471: 533-538, 2016.
34. Obayashi N, Arai K, Nakano N, Mizukami T, Kawai T, Yamamoto S, Shimizu H, Nunoi H, Shimizu T, Tang J,
Onodera M: Leopard skin-like colonic mucosa: A novel endoscopic finding of chronic granulomatous diseaseassociated colits. J. Pediatr Gastroenteol Nutr 62: 56-59, 2016.
35. Ichida Y, Utsunomiya Y, Onodera M: The third to fifth zinc fingers play an essential role in the binding of
ZFP809 to the MLV-derived PBS. BBRC 469: 490-494, 2016.
36. Tomono T, Hirai Y, Okada H, Adachi K, Ishii A, Shimada T, Onodera M, Tamaoka A, Okada T:
Ultracentrifugation-free chromatography-mediated large-scale purification of recombinant adeno-associated virus
serotype 1 (rAAV1). Mol Ther Methods Clin Dev 3: 15058, 2016.
37. Tsumura H, Ito M, Takami M, Arai M, Li XK, Hamatani T, Igarashi A, Takada S, Miyado K, Umezawa A, Ito
Y,㻌 Conditional deletion of CD98hc inhibits osteoclast development. Biochemistry and Biophysics Reports, 5,203210,2016― 297 ―




Bio-Environmental Response

38. Terao M, Tamano M, Hara S, Kato T, Kinoshita M, Takada S, Utilization of the CRISPR/Cas9 system for theefficient production of mutant mice using crRNA/tracrRNA with Cas9 nickase and FokI-dCas9. Experimental
Animals, 65,275-283,2016
39. Miyamoto Y, Tamano M, Torii T, Kawahara K, Nakamura K, Tanoue A, Takada S, Yamauchi J, Data
supporting the role of Fyn in initiating myelination in the peripheral nervous system. Data in Brief, 1,10981105,2016
40. Fujinaga H, Fujinaga H, Watanabe N, Kato T, Tamano M, Terao M, Takada S, Ito Y, Umezawa A, Kuroda M,
Cord Blood-Derived Endothelial Colony-Forming Cell Function is Disrupted in Congenital Diaphragmatic Hernia.
American journal of Physiology, Lung cellular and molecular physiology, 310,L1143-1154,2016
41. Okamura K, Sakaguchi H, Sakamoto-Abutani R, Nakanishi M, Nishimura K, Yamazaki-Inoue M, Ohtaka M,
Periasamy VS, Alshatwi AA, Higuchi A, Hanaoka K, Nakabayashi K, Takada S, Hata K, Toyoda M, Umezawa A,
Distinctive features of single nucleotide alterations in induced pluripotent stem cells with different types of DNA
repair deficiency disorders. Scientific Reports, 6,26342,2016
42. Hara S, Kato T, Goto Y, Kubota S, Tamano M, Terao M, Takada S, Microinjection-based generation of mutant
mice with a double mutation and a 0.5 Mb deletion in their genome by the CRISPR/Cas9 system. Journal of
Reproduction and Development, 62,531-536,2016
43. Akutsu H, Nasu M, Morinaga S, Motoyama T, Honma N, Machida M, Yamazaki-Inoue M, Okamura K,
Nakabayashi K, Takada S, Nakamura N, Kanzaki S, Hata K, Umezawa A, In vivo maturation of human embryonicstem cell-derived teratoma over time. Regenerative Therapy, 5,31-39,2016
44. Miyado M, Inui M, Igarashi M, Katoh-Fukui Y, Takasawa K, Hakoda A, Kanno J, Kashimada K, Miyado K,
Tamano M, Ogata T, Takada S, Fukami M, The p.R92W variant of NR5A1/Nr5a1 induces testicular developmentof 46,XX gonads in humans, but not in mice: Phenotypic comparison of human patients and mutation-induced
mice. Biology of Sex Differences, 7,56,2016
45. Takeda T, Unno H, Morita H, Futamura K, Emi-Sugie M, Arae K, Shoda T, Okada N, Igarashi A, Inoue E,
Kitazawa H, Nakae S, Saito H, Matsumoto K, Matsuda A. Platelets constitutively express interleukin-33 protein
and modulate eosinophilic airway inflammation. J Allergy Clin Immunol. 2016;138:1395-1403.
46. Shoda T, Matsuda A, Arai K, Shimizu H, Morita H, Orihara K, Okada N, Narita M, Ohya Y, Saito H,
Matsumoto K, Nomura I. Sera of patients with infantile eosinophilic gastroenteritis showed a specific increase inboth thymic stromal lymphopoietin and IL-33 levels. J Allergy Clin Immunol. 2016;138:299-303.
47. Horimukai K, Morita K, Narita M, Kondo M , Kabashima S, Inoue E, Sasaki T, Niizeki H, Saito H, Matsumoto
K, Ohya Y. Transepidermal water loss measurement during infancy can predict the subsequent development of
atopic dermatitis. Allergol Int. 2016;65:103-8.
48. Fujishima H, Okada N, Matsumoto K, Fukagawa K, Igarashi A, Matsuda A, Ono J, Ohta S, Mukai H,
Yoshikawa M, Izuhara K. The usefulness of measuring tear periostin for the diagnosis and management of ocularallergic diseases. J Allergy Clin Immunol. 2016;138:459-67.
49. Fukuie T, Hirakawa S, Narita M, Nomura I, Matsumoto K, Tokura Y, Ohya Y. Potential preventive effects ofproactive therapy on sensitisation in moderate to severe childhood atopic dermatitis: a randomised, investigatorblinded, controlled study. J Dermatol. 2016;43:1283-92.
50. Morita H, Saito H, Matsumoto K, Nakae S. Regulatory roles of mast cells in immune responses. Semin
Immunopathol. 2016;38:623-9.
51. Shoda T, Futamura K, Orihara K, Emi-Sugie M, Saito H, Matsumoto K, Matsuda A. Recent advances in theroles of vascular endothelial cells in allergic inflammation. Allergol Int. 2016;65:21-9.
52. Ozeki N, Sekiya I, Muneta T, Koga H, Nakagawa Y, Mizuno M, Tsuji K, Mabuchi Y, Akazawa C, Kobayashi
E, Futamura K, Matsumoto K, Saito T. Not single but periodic injections of synovial mesenchymal stem cells
maintain viable cells in knees and inhibit osteoarthritis progression in rats. Osteoarthritis Cartilage. 2016;24:106170.
53. Shibui A, Takamori A, Tolba MEM, Nambu A, Shimura E, Yamaguchi S, Sanjoba C, Suto H, Sudo K,
Okumura K, Sugano S, Morita H, Saito H, Matsumoto K, Nakae S. IL-25, IL-33 and TSLP receptor are not criticalfor development of experimental murine malaria. Biochemis Biophysics Rep. 2016;1:191-5.
54. Nakanishi W, Hiraishi Y, Yamaguchi S, Takamori A, Morita H, Matsumoto K, Saito H, Sudo K, Yamasoba T,
Nakae S. TSLP receptor is not essential for house dust mite-induced allergic rhinitis in mice. Biochemis
Biophysics Rep. 2016;7: 119-23.
55. Hiraishi Y, Nambu A, Shibui A , Nakanishi W, Yamaguchi S, Morita H, Iikura M, McKenzie ANJ, Matsumoto
K, Sudo K, Yamasoba T, Nagase T, Nakae S. TIM-3 is not essential for development of airway inflammationinduced by house dust mite antigens. Allergol Int. 2016;65:459-65.
56. Saito K, Arai E, Maekawa K, Ishikawa M, Fujimoto H, Taguchi R, Matsumoto K, Kanai Y, Saito Y. Lipidomic
Signatures and Associated Transcriptomic Profiles of Clear Cell Renal Cell Carcinoma. Sci Rep. 2016;6:28932.
57. Kayano M, Higaki S, Satoh J. Matsumoto K, Matsubara E, Takikawa O, Niida S.: Plasma microRNAbiomarker detection for mild cognitive impairment using differential correlation analysis. Biomarker Research.
2016:4;22.
58. Matsumoto K, Izuhara K.: Anaphylaxis-two stories not covered by the current guidelines. Allergol Int.
2016;65:1-2.
― 298 ―

Bio-Environmental Response

59. Kitade S, Onoyama I, Kobayashi H, Yagi H, Yoshida S, Kato M, Tsunematsu R, Asanoma K, Sonoda K, Wake
N, Hata K, Nakayama KI, Kato K : FBXW7 is involved in the acquisition of the malignant phenotype in epithelial
ovarian tumors. Cancer Sci. 2016;107:1399-1405
60. Kawai T, Hata K : Reproductive/Developmental Abnormalities Induced by Epigenetic Aberrations andPossible Environmental Causes. Nihon Eiseigaku Zasshi. 2016;71:195-199
61. Shima H, Yatsuga S, Nakamura A, Sano S, Sasaki T, Katsumata N, Suzuki E, Hata K, Nakabayashi K,
Momozawa Y, Kubo M, Okamura K, Kure S, Matsubara Y, Ogata T, Narumi S, Fukami M : NR0B1 Frameshift
Mutation in a Boy with Idiopathic Central Precocious Puberty. Sex Dev. 2016;10:205-209
62. Morita S, Noguchi H, Horii T, Nakabayashi K, Kimura M, Okamura K, Sakai A, Nakashima H, Hata K,
Nakashima K, Hatada I : Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1
catalytic domain fusions. Nat Biotechnol. 2016;34:1060-1065
63. Murakami M, Yoshimoto T, Nakano Y, Tsuchiya K, Minami I, Bouchi R, Fujii Y, Nakabayashi K, Hashimoto
K, Hata K, Kihara K, Ogawa Y : Expression of inflammation-related genes in aldosterone-producing adenomas
with KCNJ5 mutation. Biochem Biophys Res Commun. 2016;476:614-619
64. Okamura K, Sakaguchi H, Sakamoto-Abutani R, Nakanishi M, Nishimura K, Yamazaki-Inoue M, Ohtaka M,
Periasamy VS, Alshatwi AA, Higuchi A, Hanaoka K, Nakabayashi K, Takada S, Hata K, Toyoda M, Umezawa A :Distinctive features of single nucleotide alterations in induced pluripotent stem cells with different types of DNA
repair deficiency disorders. Sci Rep. 2016;6:26342
65. Sano S, Matsubara K, Nagasaki K, Kikuchi T, Nakabayashi K, Hata K, Fukami M, Kagami M, Ogata T :Beckwith-Wiedemann syndrome and pseudohypoparathyroidism type Ib in a patient with multilocus imprinting
disturbance: a female-dominant phenomenon? J Hum Genet. 2016;61:765-769
66. Higasa K, Miyake N, Yoshimura J, Okamura K, Niihori T, Saitsu H, Doi K, Shimizu M, Nakabayashi K, Aoki
Y, Tsurusaki Y, Morishita S, Kawaguchi T, Migita O, Nakayama K, Nakashima M, Mitsui J, Narahara M, Hayashi
K, Funayama R, Yamaguchi D, Ishiura H, Ko WY, Hata K, Nagashima T, Yamada R, Matsubara Y, Umezawa A,
Tsuji S, Matsumoto N, Matsuda F : Human genetic variation database, a reference database of genetic variations in
the Japanese population. J Hum Genet. 2016;61:547-553
67. Rumbajan JM, Yamaguchi Y, Nakabayashi K, Higashimoto K, Yatsuki H, Nishioka K, Matsuoka K, Aoki S,
Toda S, Takeda S, Seki H, Hatada I, Hata K, Soejima H, Joh K : The HUS1B promoter is hypomethylated in theplacentas of low-birth-weight infants. Gene. 2016;583:141-146
68. Morita S, Nakabayashi K, Kawai T, Hayashi K, Horii T, Kimura M, Kamei Y, Ogawa Y, Hata K, Hatada I :Gene expression profiling of white adipose tissue reveals paternal transmission of proneness to obesity. Sci Rep.
2016;6:21693
69. Masuda A, Katoh N, Nakabayashi K, Kato K, Sonoda K, Kitade M, Takeda S, Hata K, Tomikawa J : An
improved method for isolation of epithelial and stromal cells from the human endometrium. J Reprod Dev.
2016;62:213-218
70. Yoneda N, Yoneda S, Niimi H, Ueno T, Hayashi S, Ito M, Shiozaki A, Urushiyama D, Hata K, Suda W, Hattori
M, Kigawa M, Kitajima I, Saito S : Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma andOther Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm
Labor. Am J Reprod Immunol. 2016;75:112-125
71. Ito Y, Maehara K, Kaneki E, Matsuoka K, Sugahara N, Miyata T, Kamura H, Yamaguchi Y, Kono A,
Nakabayashi K, Migita O, Higashimoto K, Soejima H, Okamoto A, Nakamura H, Kimura T, Wake N, Taniguchi T,
Hata K : Novel Nonsense Mutation in the NLRP7 Gene Associated with Recurrent Hydatidiform Mole. Gynecol
Obstet Invest. 2016;81;353-358
72. Maupetit-Méhouas S, Montibus B, Nury D, Tayama C, Wassef M, Kota SK, Fogli A, Cerqueira Campos F,
Hata K, Feil R, Magueron R, Nakabayashi K, Court F, Arnaud P : Imprinting control regions (ICRs) are marked bymono-allelic bivalent chromatin when transcriptionally inactive. Nucleic Acids Res. 2016;44:621-635
73. Nohara K, Okamura K, Suzuki T, Murai H, Ito T, Shinjo K, Takumi S, Michikawa T, Kondo Y, Hata K :Augmenting effects of gestational arsenite exposure of C3H mice on the hepatic tumors of the F2 male offspring
via the F1 male offspring. J Appl Toxicol. 2016;36:105-112
74. Hayashi K, Kawai YL, Yura K, Yoshida MA, Ogura A, Hata K, Nakabayashi K, Okamura K : Complete
genome sequence of the mitochondrial DNA of the sparkling enope squid, Watasenia scintillans. MitochondrialDNA. 2016;27:1842-1843

>5HYLHZ$UWLFOHV@

1. Fukami M, Seki A, Ogata T. SHOX Haploinsufficiency as a cause of syndromic and non-syndromic shortstature. Mol Syndromol 7(1): 3–11, 2016
2. Fukami M, Shima H, Suzuki E, Ogata T, Matsubara K, Kamimaki T, Catastrophic Cellular Events Leading toComplex Chromosomal Rearrangements in the Germline. Clin Genet 2016 (in press)>%RRNV@
― 299 ―

Systemic Organ Regulation

Systemic Organ Regulation

Human Pathology

Professor: Yoshinobu EISHI
Assistant Professor: Daisuke KOBAYASHI,Takashi ITO,Mariko NEGI
Laboratory Technician: Asuka FURUKAWA
Technical Assistant: Yuki ISHIGE
Graduate Students:(Doctor Program) Yoshimi SUZUKI,Katsumi OISHI,Akira TAKEMOTO,
Tomohisa OGAWA,Tomohito AYABE,Nobuyasu AWANO,
Tomoya KAKEGAWA,Yuji SEKINE,Kurara AKITA
Masahiro YAMAMOTO(MD-PhD course) Yuriko WADA
(Master’s Program) Daisuke SAKONISHI,Momoko SAKAGUCHI
Reserch Student: Yuka HIROTA
Secretary: Miho IWAMITSU,Mayako TOKUNAGA(1)OutlinePathology in a medical department used to be the general study ﬁeld about human disease. Later, Microbiology
and Parasitology had been separated from the ﬁeld of Pathology. Lately, interdisciplinary of traditional study
ﬁeld had been advanced and new speciﬁc academic disciplines are developed. Pathology is currently under thesame trend. Basic divisions such as Experimental and Cell Biological pathology are becoming independent fromthe clinicopathological ﬁeld. Thus, Human Pathology has become the main category of pathology.
The principles of Human Pathology are to educate clinical pathologists with accurate pathological diagnosis
skills of human disease, to research theses that are directly related to human disease, and to educate pathologicalresearchers with ability to perform such research.

(2)

Research

1)Endogenous infection (diseases caused by indigenous microorganisms in susceptible hosts)2)Cancer research (histopathology, carcinogenesis, prognostic factors, and so on)(3)

Education

In the course, they usually spend the ﬁrst two years for anatomical pathology training, searching for their own
research theme and another two years for researches and thesis-writing.
(4)

Lectures & Courses

Department of Human Pathology provides a graduate course for future pathologists to train the skills andknowledge of anatomical pathology and develop the abilities for medical researches. Graduate students are― 300 ―

Systemic Organ Regulation

Systemic Organ Regulation

educated to associate their researches with problems in diagnosis and treatment of diseases and etiologies of thediseases of unknown causes.
(5)

Clinical Performances

After the Meiji Era, the department of Human Pathology in medical faculty belonged under the basic medical
sciences; however, Pathology in the existing hospitals is essentially the clinical medicine. Diagnosis of patientsin each clinical department is done by taking the biopsy of diseased tissues or collecting the cell samples byeither endoscope or surgery. Then, the lesions are analyzed with the microscope, and pathological diagnosis
is reported to the clinical departments. The samples of organs and tissues taken from the surgery are used to
study the spread of the lesion and its characteristics, and also to examine the adequacy of surgery. It is also
used to determine future treatment policy. During the course of patients treatments, sample tissues are taken
periodically and are analyzed pathologically to see therapeutic eﬀect. If a patient has unfortunately joined the
majority, morbid anatomy is done by the pathologists along with the patient s attending physician. They
study the resulting eﬀects of laboratory ﬁndings and choice of treatment, and improve the future diagnosis andtreatments. The department of Human Pathology and Surgical Pathology technically work as one although
they are separated in this university s organizational structure. Human Pathology does not directly work withthe patients; however, it is involved directly with the diagnosis as well as the treatments. Strong cooperation
between clinicians and pathologists is essential for the best practice, and is required for the university hospitalas an advanced treatment hospital. Therefore, doctors of Human Pathology study, research and practicepathology to be the great pathologists so-called the doctor of doctors.
(6)

Publications[Original Articles]
1. Wakaba Yamaguchi, Naofumi Yui, Toshikage Nagao, Gaku Oshikawa, Mariko Negi, Soichiro Iimori,Tomokazu Okado, Tatemitsu Rai, Shinichi Uchida. Bence-Jones Protein λ-type Multiple Myeloma
Patient Withdrawn from Maintenance Hemodialysis after Long-term Bortezomib and Dexamethasone
Therapy. Intern. Med.. 2016; 55(3); 263-268
2. Nobuo Sanjo,Satoko Kina,Yukiko Shishido-Hara,Yurie Nose,Satoru Ishibashi,Tetsuya Fukuda,Taketoshi
Maehara,Yoshinobu Eishi,Hidehiro Mizusawa,Takanori Yokota. A Case of Progressive Multifocal Leukoencephalopathy with Balanced CD4/CD8 T-Cell Inﬁltration and Good Response to Meﬂoquine Treatment.
Internal Medicine. 2016.01; 55(12); 1631-1635
3. Akiyama Y, Koda Y, Byeon SJ, Shimada S, Nishikawaji T, Sakamoto A, Chen Y, Kojima K, Kawano
T, Eishi Y, Deng D, Kim WH, Zhu WG, Yuasa Y, Tanaka S. Reduced expression of SET7/9, a histone
mono-methyltransferase, is associated with gastric cancer progression. Oncotarget. 2016.01; 7(4); 39663983
4. Okada T, Tanaka K, Kawachi H, Ito T,Nishikage T,Odagaki T,Zárate AJ, Kronberg U, López-Köstner
F,Karelovic S, Flores S, Estela R, Tsubaki M, Uetake H, Eishi Y, Kawano T. International collaboration
between japan and chile to improve detection rates in colorectal cancer screening. Cancer. 2016.01; 122(1);71-77
5. Kamoi K, Nagata Y, Mochizuki M, Kobayashi D, Ohno N, Uchimaru K, Tojo A, Ohno-Matsui K.. Formation of Segmental Rounded Nodules During Inﬁltration of Adult T-Cell Leukemia Cells Into the Ocular
Mucous Membrane. Cornea. . 2016.01; 35(1); 137-139
6. Yamada I, Hikishima K, Miyasaka N, Kato K, Kojima K, Kawano T, Ito E, Kobayashi D, Eishi Y, Okano
H. Gastric carcinoma: evaluation with diﬀusion-tensor MR imaging and tractography ex vivo. Magn
Reson Imaging. 2016.02; 34(2); 144-151
7. Tadahisa Numakura, Tsutomu Tamada, Masayuki Nara, Soshi Muramatsu, Koji Murakami, Toshiaki
Kikuchi, Makoto Kobayashi, Miho Muroi, Tatsuma Okazaki, Sho Takagi, Yoshinobu Eishi, Masakazu
Ichinose. Simultaneous development of sarcoidosis and cutaneous vasculitis in a patient with refractory
Crohn’s disease during inﬂiximab therapy. BMC Pulm Med. 2016.02; 16; 30― 301 ―

Systemic Organ Regulation

Systemic Organ Regulation

8. Mukawa, M. Nariai, T. Inaji, M. Tamada, N. Maehara, T. Matsushima, Y. Ohno, K. Negi, M. Kobayashi,
D.. First autopsy analysis of a neovascularized arterial network induced by indirect bypass surgery formoyamoya disease J Neurosurg. 2016.05; 124(5); 1211-1214
9. Atsuko Konta, Kouichi Ozaki, Yasuhiko Sakata, Atsushi Takahashi, Takashi Morizono, Shinichiro Suna,
Yoshihiro Onouchi, Tatsuhiko Tsunoda, Michiaki Kubo, Issei Komuro, Yoshinobu Eishi, Toshihiro Tanaka.
A functional SNP in FLT1 increases risk of coronary artery disease in a Japanese population. J. Hum.
Genet.. 2016.05; 61(5); 435-441
10. Makiko Egawa, Naoyuki Miyasaka, Takuyuki Kubo, Miho Naitou, Takashi Ito, Toshiro Kubota. A caseof puerperal group a streptococcal sepsis complicated by ovarian vein thrombosis. J Obstet Gynaecol.
2016.05; 1-5
11. Ryohei Hayashi, Kiichiro Tsuchiya, Keita Fukushima, Nobukatsu Horita, Shuji Hibiya, Keisuke Kitagaki,
Mariko Negi, Eisaku Itoh, Takumi Akashi, Yoshinobu Eishi, Eriko Okada, Akihiro Araki, Kazuo Ohtsuka,
Shinji Fukuda, Hiroshi Ohno, Ryuichi Okamoto, Tetsuya Nakamura, Shinji Tanaka, Kazuaki Chayama,
Mamoru Watanabe. Reduced Human α-defensin 6 in Noninﬂamed Jejunal Tissue of Patients with Crohn’s
Disease. Inﬂamm. Bowel Dis. 2016.05; 22(5); 1119-1128
12. Nariaki Kokuho, Yasuhiro Terasaki, Hirokazu Urushiyama, Mika Terasaki, Shinobu Kunugi, Taisuke
Morimoto, Arata Azuma, Jitsuo Usuda, Akihiko Gemma, Yoshinobu Eishi, Akira Shimizu. Pulmonary
mucosa-associated lymphoid tissue lymphoma associated with pulmonary sarcoidosis: a case report andliterature review. Hum. Pathol.. 2016.05; 51; 57-63
13. Teruko Nakamura, Asuka Furukawa, Keisuke Uchida, Tomohisa Ogawa, Tomoki Tamura, Daisuke Sakonishi, Yuriko Wada, Yoshimi Suzuki, Yuki Ishige, Junko Minami, Takumi Akashi, Yoshinobu Eishi. Autophagy Induced by Intracellular Infection of Propionibacterium acnes. PLoS ONE. 2016.05; 11(5);e0156298
14. Masahiro Asakawa, Takanobu Yoshimoto, Mitsutane Ota, Mitsuyuki Numasawa, Yuriko Sasahara, Takato
Takeuchi, Yujiro Nakano, Norihiko Oohara, Masanori Murakami, Ryotaro Bouchi, Isao Minami, Kyoichiro Tsuchiya, Koshi Hashimoto, Hajime Izumiyama, Naoko Kawamura, Kazunori Kihara, Mariko
Negi, Takumi Akashi, Yoshinobu Eishi, Hironobu Sasano, Yoshihiro Ogawa. A Case of Cushing’s Syndrome with Multiple Adrenocortical Adenomas Composed of Compact Cells and Clear Cells. Endocr.
Pathol.. 2016.06; 27(2); 136-141
15. Eriko Katsuta, Atsushi Kudo, Takumi Akashi, Yusuke Mitsunori, Satoshi Matsumura, Arihiro Aihara,
Daisuke Ban, Takanori Ochiai, Shinji Tanaka, Yoshinobu Eishi, Minoru Tanabe. Macroscopic morphology
for estimation of malignant potential in pancreatic neuroendocrine neoplasm. J. Cancer Res. Clin. Oncol..
2016.06; 142(6); 1299-1306
16. Sanae Shimamura, Naoto Yokogawa, Kengo Murata, Tetsuo Yamaguchi, Keisuke Uchida, Yoshinobu Eishi.
Saddle nose with sarcoidosis: "A great imitator" of relapsing polychondritis. Mod Rheumatol. 2016.06;
1-5
17. Yamada I, Hikishima K, Miyasaka N, Kato K, Ito E, Kojima K, Kawano T, Kobayashi D, Eishi Y, Okano
H. q-Space MR imaging of gastric carcinoma ex vivo: correlation with histopathologic ﬁndings. Magn
Reson Med. 2016.08; 76(2); 602-612
18. Ryuichi Okamoto, Mariko Negi, Syohei Tomii, Yoshinobu Eishi, Mamoru Watanabe. Diagnosis andtreatment of microscopic colitis. Clin J Gastroenterol. 2016.08; 9(4); 169-174
19. Nishikawaji T, Akiyama Y, Shimada S, Kojima K, Kawano T, Eishi Y, Yuasa Y, Tanaka S. Oncogenic rolesof the SETDB2 histone methyltransferase in gastric cancer. Oncotarget. 2016.10; 7(41); 67251-67265
20. Saori R Yoshii, Akiko Kuma, Takumi Akashi, Taichi Hara, Atsushi Yamamoto, Yoshitaka Kurikawa,
Eisuke Itakura, Satoshi Tsukamoto, Hiroshi Shitara, Yoshinobu Eishi, Noboru Mizushima. Systemic
Analysis of Atg5-Null Mice Rescued from Neonatal Lethality by Transgenic ATG5 Expression in Neurons.
Dev. Cell. 2016.10; 39(1); 116-130[Conference Activities & Talks]
1. Yoshinobu EISHI. ABC method clinical trial protocol. Thai gastric cancer society meeting 2016.08.22
Bangkok, Thailand― 302 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

Physiology and Cell Biology

Lecturer: Shingo Sato
Assistant professor: Hiroki Ochi
Assistant professor: Satoko Sunamura(1)

OutlineRecent progress in molecular biology and genetics advanced our understanding of molecular basis of physiological function and pathophysiological mechanisms of various diseases. Besides, signal transduction systemusing intercellular, intersystem, and inter-organ networks has been shown to be essential for whole-body homeostatic function. In our department, we are studying on the inter-organ regulatory networks of metabolism,
especially between bone and the other organs.
(2)

Research

1. Studies on the regulatory network of metabolism between bone and the other internal organs: It had
been believed that bone is controlled by local environment through the action of hormones and cytokines,
independently of the other organs. However, our discovery that leptin regulates bone formation through thecentral nervous system shed light on a new regulatory system of bone metabolism, i.e., neuronal control (Takeda
S, Cell, 2002、Nature, 2005). In addition, we have also demonstrated that neuromedin U, an anorexigenic
neuropeptide, regulates bone formation through the central nervous system (Sato S, Nat Med, 2007). Moreover,recent studies have revealed that FGF23 or osteocalcin, which is secreted by bone, regulates the metabolism ofkidney or pancreas. Thus, bone is now considered as a major player for whole-body homeostasis, and forms a
regulatory network of metabolism together with the other organs. We are now conducting further experiments
to clarify a comprehensive network between bone and the other organs.
2. Studies on the regulation of bone metabolism by sensory nerves: We have recently revealed that sensory
nerves inside bones have a crucial role in regulating bone mass, and that the penetration of sensory nerves intobones is necessary for normal bone development or fracture healing (Fukuda T, Nature, 2013). Based on theseﬁndings, we are now conducting further experiments to develop novel therapeutic approaches to osteoporosis.
3. Studies on the regulation of bone metabolism and bone metastasis by microRNA: microRNA (miRNA) isa small non-coding RNA molecule, and regulates various developmental and homeostatic events in vertebrates
and invertebrates. Aberrant expression of miRNA has been implicated in numerous disease states, and miRNAbased therapies are under investigation. We have previously demonstrated the physiological role of miRNA in
osteoblast diﬀerentiation (PNAS, 2009). We are now conducting further experiments to identify novel miRNAs
regulating bone metabolism or bone metastasis.
4. Studies on the mechanical regulation of musculoskeletal system: In Japan, the number of patients withosteoporosis or sarcopenia have rapidly increased. Mechanical stress is known to be essential for the maintenanceof bone volume or muscle strength. However, the mechanism of the mechanical regulation remains elusive. We
are now elucidating the role of gravity on bone or muscle homeostasis as well as on neurovascular formation inbone or muscle tissues by utilizing a tail suspension mouse model.
5. Studies on the mechanism in the development of bone and soft tissue sarcomas: Sarcomas are malignancies― 303 ―

Systemic Organ
Organ Regulation
Regulation
Systemic

derived from mesenchymal tissues such as bone, muscle, fat, and cartilage. Molecular mechanisms in theoccurrence and growth of sarcomas have yet to be elucidated. Even the cell of origin for most sarcomas still
remains unclear. We have recently established a novel mouse model for osteosarcoma and also demonstrated
that sarcomas could be derived from pericytes with mutations of crucial genes. Based on these ﬁndings, we are
now conducting further experiments to elucidate the detailed mechanism of sarcoma development.
(3)

Education

We give lectures and laboratory teachings about physiology to sophomore medical students. We also teachexperimental techniques to a lot of undergraduate students to develop young basic scientist. PhD students
are required to join our research team and learn various experimental techniques including molecular biology,
cellular biology, and physiology.
(4)

Lectures & Courses

All students are expected to understand the background of the research ﬁeld and bring up relevant scientiﬁcquestions to verify the hypothesis. They are also expected to develop their scientiﬁc thinking with eﬀective questions and cultivate their abilities to analyze obtained results objectively, discuss them logically and scientiﬁcally,and present them eﬀectively.
(5)

Publications[Original Articles]
1. Yoshinori Asou, Munetaka Iwata, Hiroki Ochi, Maierhaba Ailixiding, Zulipiya Aibibula, Jinying Piao,
Guangwen Jin, Yasushi Hara, Atsushi Okawa. Pleiotropic Functions of High Fat Diet in the Etiology of
Osteoarthritis. PLoS ONE. 2016; 11(9); e0162794
2. Cheng Xu, Hiroki Ochi, Toru Fukuda, Shingo Sato, Satoko Sunamura, Takeshi Takarada, Eiichi Hinoi,
Atsushi Okawa, Shu Takeda. Circadian clock regulates bone resorption in mice J. Bone Miner. Res..
2016.02; 31(7); 1344-1355
3. Masanori Saito, Mieradili Mulati, S Zakiah A Talib, Philipp Kaldis, Shu Takeda, Atsushi Okawa, Hiroyuki
Inose. The Indispensable Role of Cyclin-Dependent Kinase 1 in Skeletal Development. Sci Rep. 2016.02;
6; 20622
4. Yoichi Imai, Eri Ohta, Shu Takeda, Satoko Sunamura, Mariko Ishibashi, Hideto Tamura, Yan-Hua Wang,
Atsuko Deguchi, Junji Tanaka, Yoshiro Maru, Toshiko Motoji. Histone deacetylase inhibitor panobinostat
induces calcineurin degradation in multiple myeloma. JCI Insight. 2016.04; 1(5); e85061
5. Sato S, Tang YJ, Wei Q, Hirata M, Weng A, Han I, Okawa A, Takeda S, Whetstone H, Nadesan P,Kirsch
DG, Wunder JS, Alman BA. Mesenchymal tumors can derive from Ng2/Cspg4-expressing pericytes with
β-catenin modulating the neoplastic phenotype Cell Reports. 2016.07; 16(4); 917-927
6. Takashi Iezaki, Kakeru Ozaki, Kazuya Fukasawa, Makoto Inoue, Shigetaka Kitajima, Takeshi Muneta,
Shu Takeda, Hiroyuki Fujita, Yuki Onishi, Tetsuhiro Horie, Yukio Yoneda, Takeshi Takarada, Eiichi Hinoi.
ATF3 deﬁciency in chondrocytes alleviates osteoarthritis development. J. Pathol.. 2016.08; 239(4); 426437
7. Takeshi Takarada, Cheng Xu, Hiroki Ochi, Ryota Nakazato, Daisuke Yamada, Saki Nakamura, Ayumi
Kodama, Shigeki Shimba, Michihiro Mieda, Kazuya Fukasawa, Kakeru Ozaki, Takashi Iezaki, Koichi Fujikawa, Yukio Yoneda, Rika Numano, Akiko Hida, Hajime Tei, Shu Takeda, Eiichi Hinoi. Bone Resorption
Is Regulated by Circadian Clock in Osteoblasts. J. Bone Miner. Res.. 2016.12;
8. Masahiro Niimura, Tsuyoshi Sato, Yuichiro Enoki, Masahiko Okubo, Shoichiro Kokabu, Shu Takeda,
Tetsuya Yoda. Semaphorin 3A Promotes Dendrite Elongation of Osteocytes in Association with Downregulation of CDK6. In Vivo. 30(3); 231-236― 304 ―

Systemic
Regulation
Systemic Organ
Organ Regulation[Misc]
1. Shingo Sato, Shu Takeda. RANKL/RANK/OPG genetically engineered mice PharmaNavigator. 2016;
2. Toru Fukuda, Shu Takeda. Regulation of bone homeostasis by nerve system The medical frontline.
2016.02; 71(2); 223-229
3. Sato S, Takeda S. The regulation of various organs by osteoblasts Clinical Calcium. 2016.05; 26(5);721-727[Conference Activities & Talks]
1. Yukihiko Tamura, Setsuko Mise, Yasutaka Sugamori, Md. Zahirul Haq Bhuyan, Mariko Takahashi,
Tomoki Uehara, Yuki Arai, Michiyo Miyashin, Noriyuki Wakabayashi, Kazuhiro Aoki. A pharmacological role of metallothionein in zinc-treated cartilage-progenitor cells. APFP 2016(The 13th Asia Paciﬁc
Federation of Pharmacologists Meeting) 2016.02.01 The Berkeley Hotel Pratunam,Bangkok (Thailand)
2. Shingo Sato. The cell of origin of bone and soft tissue sarcomas. 242th Kanto musculoskeletal tumor
workshop 2016.03.08 Tokyo
3. Shingo Sato, Takashi Hirai, Dai Ukegawa, Seiichi Matsumoto, Shu Takeda, Atsushi Okawa. Reduction in
the frequency of emergency surgery by medical and dental combined treatment system for bone metastasis.
The 49th Annual Musculoskeletal Tumor Meeting of the Japanese Orthopaedic Association 2016.07.14
Tokyo
4. Shingo Sato, Benjamin Alman, Shu Takeda, Atsushi Okawa. The same cellular origin of musculoskeletal
tumor and pituitary tumor. The 31st Annual Research Meeting of the Japanese Orthopaedic Association
2016.10.13 Fukuoka
5. Shingo Sato, Yuning J. Tang, Qingxia Wei, Makoto Hirata, Ilkyu Han, Shu Takeda, David G. Kirsch, Jay
S. Wunder, Benjamin A. Alman. Mesenchymal tumors can derive from NG2-expressing pericytes withbeta-catenin modulating the neoplastic phenotype. Connective Tissue Oncology Society Meeting 20162016.11.11 Lisbon, Portugal
6. Shingo Sato, Hiroki, Ochi, Shu Takeda. Novel molecular regulation of postmenopausal osteoporosis by
the inter-organ metabolic network. The 39th Annual Meeting of the Molecular Biology Society of Japan
2016.12.02 Yokohama
7. Shingo Sato, Hiroki Ochi, Shu Takeda. Regulation of whole body homeostasis by organ-network betweenbone and other organs. Cardiovascular and Metabolic Week (CVMW) 2016 2016.12.16 Tokyo― 305 ―

Systemic Organ Regulation

Systemic Organ Regulation

Molecular Cellular Cardiology

Professor
Assistant professor
Post-doc (RPD)
Post-doc (PD)Post-doc
D4(Dept. Cardiovascular Medicine)
D4
M2
M2
M1
M1
Technician
Technician
Secretary(1)

Tetsushi Furukawa
Kensuke Ihara
Masami Kodama
Nozomi Hayashiji
Kentaro Takahashi
Masahiro Yamazoe
Lian Liu
Shun Fukuda
Xiaoki Yang
Shuhei Ishii
Sun Yihan
Tomoko Ando
Reiko Kimura
Kuniko Yamaguchi

OutlineThis laboratory focuses on understanding pathogenesis of intractable and common cardiovascular diseases using
multidisciplinary approach (patch-clamp, cell biology, optical recording, genetic analysis, and computational
analysis). Our ultimate goal is to improve diagnosis and management of intractable and common cardiovasculardiseases.
(2)

Research

1. Pathogenesis of atrial ﬁbrillation (AF)Up to the present, we have carried 3 GWAS (genome-wide association study); 1st GWAS with 250K SNPs, 2nd
GWAS with 610K SNPs, and 3rd GWAS with 1000K SNPs. The 1st GWAS gave us 2 AF-sensitive SNPs, the
2nd GWAS 13 AF-sensitive SNPs (Nat. Genet. 2012;44;670-675, Circulation 2014;130:1225-1235), and the 3rd
GWAS 16 AF-sensitive SNPs (under revision). Using the SNPs identiﬁed in the 2nd GWAS, we constructedweighed genetic risk score (GRS), which could predict AF development with 60% sensitivity and 61% speciﬁcity(Can. Heart J. 2017 [ in press] , J. Cardiol. 2017 [ in press] ).
2. Pannexin for ischemic preconditioning
Pannexin is a member of gap-junction channel, which is not involved in the formation of gap-junction channels
between cells, but provides hemi-channels on the surface of the membrane. Pannexin functions as chloride channels in the basal condition and as ATP-release channels after mechanical stimuli. Being suﬀered from myocardial
infarction, hearts with prior ischemic events (angina) develop reduced size infarcted size compared with those
without prior ischemic events. The eﬀect of prior ischemic events is referred as ischemic pre-conditioning .
Though extracellular ATP is known to play an important role for ischemic pre-conditioning, the source of ATP
is not known. Using pannexin-1 (a major isoform of pannexin in hearts) KO mouse, we found that ATP released
during hypoxia was much lower in KO mice than in WT mice. Ischemic pre-conditioning reduced the infarcted
area in WT mice, which was almost abolished in KO mice. Taken together, we conclude that hypoxia-activatedpannexin provides extracellular ATP, which is required for the ischemic pre-conditioning.

― 306 ―

Systemic Organ Regulation

Systemic Organ Regulation

3. Safety cardiac pharmacology and toxicology using iPS cells and mathematical modeling
We aim to contribute to assessments for drug-induced lethal arrhythmias which have been a major reason for
drug withdrawal from market. Novel multidisciplinary approaches using in silico mathematical models (collaborating with Dr. Takashi Ashihara at Shiga University of Medical Science) and human iPS cells-derived
cardiomyocytes (collaborating with Dr. Yasunari Kanda and Dr. Yuko Sekino at National Institute of Health
Sciences) are developing in order to predict lethal drug-induced arrhythmias at pre-clinical safety pharmacological and toxicological tests. In this year, we developed a simulation model which can describe automaticity ofelectrical activity of human iPS cells-derived cardiomyocytes, and found that KCNJ2 expression is crucial forthe automaticity (under revision).
4.Cardiac development and regeneration
The technologies for cardiomyocyte induction from human iPSCs gave us one of progressive tools for clinical application. However, the cardiomyocyte characters greatly vary by their diﬀerentiation time and theirplaces. Even long-cultured cardiomyocytes form human iPSCs still keep immature characters with arrhythmia
or tachycardia. To address this issue, we identiﬁed that a novel transcription factor, Sall1 acts as a key player
for heart induction via regulation of major cardiac genes such as Islet1/Nkx2-5/Tbx5/Gata4/Mef2c etc (Morita
et al., JMCC 2016), and cooperatively functions with a cardiac gene for heart speciﬁcation and its maturation(Morita et al., submitted 2017). We also found that these genes function during cardiac regeneration in mammals (Nakamura et al., Develop. Growth. Diﬀer. 2016). In addition, to address the mechanisms of congenital
heart defects and heart failure in adults, we found the evidence that some epigenetic genes from RNA-sequence
between males and females in human/rodents might enhanced these symptoms more worse (Tsuji et al., revised
2017; Hori et al., submitted 2017).

(3)

Education

School of Medicine
2nd grade Introduction to Neurophysiology (2 units)
2nd grade Physiology (6 units)
3rd grade Cardiology (1 unit)
4th grade Project semester
School of Dentistry
3rd grade Pharmacology III(2 units)
3rd grade Practice for Pathophysiological Sciences (2 units)
School of Health Care Medicine
3rd/4th grade Cardiac physiology (8 units)(4)

Publications[Original Articles]
1. Yuki Hasegawa, Satomi Hamada, Takuro Nishimura, Takeshi Sasaki, Yusuke Ebana, Mihoko Kawabata,
Masahiko Goya, Mitsuaki Isobe, Takatoshi Koyama, Tetsushi Furukawa, Kenzo Hirao, Tetsuo Sasano.
Novel Dielectric Coagulometer Identiﬁes Hypercoagulability in Patients with a High CHADS2 Score without Atrial Fibrillation. PLoS ONE. 2016; 11(6); e0156557
2. Soichiro Yoshikawa, Takako Usami, Junichi Kikuta, Masaru Ishii, Tetsuo Sasano, Koji Sugiyama, Tetsushi
Furukawa, Eiji Nakasho, Hiroshi Takayanagi, Thomas F Tedder, Hajime Karasuyama, Atsushi Miyawaki,
Takahiro Adachi. Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic
mice: indication of autoimmune diseases before the pathological onset. Sci Rep. 2016.01; 6; 18738
3. Kentaro Takahashi, Tetsuo Sasano, Koji Sugiyama, Junko Kurokawa, Noriko Tamura, Yurie Soejima, Motoji Sawabe, Mitsuaki Isobe, Tetsushi Furukawa. High-fat diet increases vulnerability to atrial arrhythmia
by conduction disturbance via miR-27b. J. Mol. Cell. Cardiol.. 2016.01; 90; 38-46
4. Lopez-Redondo F, Kurokawa J, Nomura F, Kaneko T, Hamada T, Furukawa T, Yasuda K.. A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived
cardiomyocytes. Journal Pharmacological Science. 2016.04; 8613(16); 30032-30039― 307 ―

Systemic Organ Regulation

Systemic Organ Regulation

5. Sugiyama K, Sasano T, Kurokawa J, Takahashi K, Okamura T, Kato N, Isobe M, Furukawa T. Oxidative
Stress Induced Ventricular Arrhythmia and Impairment of Cardiac Function in Nos1ap Deleted Mice
International Heart Journal. 2016.05; 57(3); 341-349
6. Yoichi Otaki, Yusuke Ebana, Shunji Yoshikawa, Mitsuaki Isobe. Dielectric permittivity change detects
the process of blood coagulation: Comparative study of dielectric coagulometry with rotational thromboelastometry. Thromb. Res.. 2016.06; 145; 3-11
7. Hirokazu Ohigashi, Natsuko Tamura, Yusuke Ebana, Masayoshi Harigai, Yasuhiro Maejima, Takashi
Ashikaga, Mitsuaki Isobe. Eﬀects of immunosuppressive and biological agents on refractory Takayasu
arteritis patients unresponsive to glucocorticoid treatment. J Cardiol. 2016.08;
8. Okata S, Yuasa S, Suzuki T, Ito S, Makita N, Yoshida T, Li M, Kurokawa J, Seki T, Egashira T, Aizawa
Y, Kodaira M, Motoda C, Yozu G, Shimojima M, Hayashiji N, Hashimoto H, Kuroda Y, Tanaka A,
Murata M, Aiba T, Shimizu W, Horie M, Kamiya K, Furukawa T, Fukuda K. Embryonic type Na+channel -subunit, SCN3B masks the disease phenotype of Brugada syndrome Science Reports. 2016.09;
6; 34198
9. Lian Liu, Yusuke Ebana, Jun-Ichi Nitta, Yoshihide Takahashi, Shinsuke Miyazaki, Toshihiro Tanaka,
Masatoshi Komura, Mitsuaki Isobe, Tetsushi Furukawa. Genetic Variants Associated With Susceptibility
to Atrial Fibrillation in a Japanese Population. Can J Cardiol. 2016.11;[Misc]
1. Kurokawa J, Kodama M, Clancy CE and Furukawa T. Sex hormonal regulation of cardiac ion channels
in drug-induced QT syndromes Pharmacology & Therapeutics. 2016; 168; 23-28
2. Yamazoe M, Furukawa T. Long-Term Prognosis of Catecholaminergic Polymorphic Ventricular Tachycardia Patients With Ryanodine Receptor (RYR2) Mutations 2016.08; 80(9); 1892-1894
3. Yusuke Ebana. Ethics in Clinical Research Obstet. Gynecol.. 2016.10; 83(10); 1119-1123[Conference Activities & Talks]
1. 1. Sasano T, Sugiyama K, Kurokawa J, Takahashi K, Okamura T, Kato N, Isobe M, Furukawa T. Genetic
deletion of Nos1ap induced ventricular arrhythmia and impairment of cardiac function under oxidative
stress condition. 5th International Conference of Cardiovascular Medicine 2016.03.05 Taichung, Taiwan
2. Takahashi K, Sasano T, Sugiyama K, Isobe M, Furukawa T. Contribution of pannexin-1 to cardioprotection and arrhythmogenicity induced by ischemia/reperfusion injury. The 80th Annual Scientiﬁc Meetingof the Japanese Circulation Society 2016.03.18 Sendai
3. Takahashi K, Sasano T, Sugiyama K, Orui Y, Isobe M, Furukawa T. Contribution of pannexin-1 tocardioprotection and arrhythmogenicity induced by preconditioning against ischemia/reperfusion injury.
The 80th Annual Scientiﬁc Meeting of the Japanese Circulation Society 2016.03.18 Sendai
4. Hayashi Y, Nitta J, Iwasaki T, Lee K, Watanabe K, Hirao T, Kato N, Inamura Y, Suzuki M, Negi K,
Satoh A, Yamato T, Matsumurea Y, Asakawa K, Ebana Y, Furukawa T, Gohya M, Hirao K, Isobe M.
The occurrence of ectopy from pulmonary veins in atrial ﬁbrillation in middle-aged women. The 80th
Annual Scientiﬁc Meeting of the Japanese Circulation Society 2016.03.19 Sendai
5. Ito S, Ebana Y, Furukawa T. Neurl, ubiquitin E3 ligase, contributes to the maintenance of sarcomere
structure and the regulation of autophagy. The 80th Annual Scientiﬁc Meeting of the Japanese Circulation
Society 2016.03.19 Sendai
6. Hayashi Y, Nitta J, Iwasaki T, Lee K, Watanabe K, Hirao T, Kato N, Inamura Y, Suzuki M, Negi K,
Satoh A, Yamato T, Matsumurea Y, Asakawa K, Ebana Y, Furukawa T, Gohya M, Hirao K, Isobe M.
The genotype of SNPs related whether ectopy is from pulmonary vein or others in atrial ﬁbrillation. The
80th Annual Scientiﬁc Meeting of the Japanese Circulation Society 2016.03.19 Sendai
7. Hamada S, Hasegawa Y, Nishimura T, Furukawa T, Isobe M, Hirao K, Sasano T. Novel dielectric blood
coagulometry can evaluate factor Xa and thrombin activity individually. The 80th Annual Scientiﬁc
Meeting of the Japanese Circulation Society 2016.03.20 Sendai― 308 ―

Systemic Organ Regulation

Systemic Organ Regulation

8. Yusuke Ebana Hiroko Kohbata, Sayako Takahashi, Masayuki Yoshida. Genetic counselling for a case withbreast cancer who underwent a whole-exome sequencing in a private company. The 13th International
Congress Society of Human Genetics 2016.04.06
9. Furukawa T, Liu L, Ebana Y, Nitta J, Takahashi Y, Miyazaki M, Isobe M, Tanaka T . Gene-Guided
Therapy in AF. Heart Rhythm Society’s 37th Annual Scientiﬁc Sessions 2016.05.06 San Francisco
10. Sasano T, Sugiyama K, Kurokawa J, Takahashi K, Okamura T, Kato N, Isobe M, Furukawa T. Oxidative
Stress Induced Ventricular Arrhythmia and Impairment of Cardiac Function in Nos1ap Deleted Mice.
The 9th international conference on the biology, chemistry, and therapeutic approach of Nitric Oxide
2016.05.22
11. Tetsushi Furukawa. Add-on type cardiac development and arrhythmias. Annual Meeting of the JapaneseHeart Rhythm Society 2016 July 15, 2016 2016.07.15 Sapporo, Hokkaido
12. Tetsushi Furukawa. Mechanisms of arrhythmias in heart failure and new advances in its management.
Annual Meeting of the Japanese Heart Rhythm Society 2016 2016.07.16 Sapporo, Hokkaido
13. Lian Liu, Yusuke Ebana, Jun-ichi Nitta, Yoshihide Takahashi, Shinsuke Miyazaki, Toshihiro Tanaka,
Masatoshi Komura, Mitsuaki Isobe, Tetsushi Furukawa. Common genetic variants indicate the risk of
atrial ﬁbrillation in Japanese population.. The 63th Annual Meeting of the Japanese Heart Rhythm
Society 2016.07.16 Sapporo
14. Sasano T, Hamada S, Hasegawa Y, Nishimura T, Yoshitake T, Shiohira S, Sasaki T, Kawabata M, Goya M,
Isobe M, Furukawa T, Hirao K. Individual evaluation of whole blood coagulability and factor Xa activity
by novel dielectric blood coagulometry.. The 63th Annual Meeting of the Japanese Heart Rhythm Society
2016.07.17 Sapporo
15. Kurokawa J, Kanda Y, Kodama M, Sekino Y, Furukawa T. Eﬀects of anticancer drugs on contractile
behaviors of human- induced pluripotent stem cell-derived cardiomyocyte monolayers evaluated with animage-based analysis using motion ﬁeld imaging technique. 2016 SPS/JSPS/CSPT 2016.09.19 Vancouver,Canada
16. Furukawa T, Okata S, Yuasa S, Suzuki T, Makita N, Kurokawa J, Egashira T, Yamakawa H, Seki T,
Aizawa T, Hashimoto H, Kuroda Y, Tanaka A, Yae K, Murata M, Aiba T, Shimizu W, Horie M, Kodama
I, Ogawa S, Fukuda K. Disease modeling using iPS cells. The 78th Annual Scientiﬁc Meeting of theJapanese Circulation Society Tokyo
17. Sato Y, Satoh A, Nitta J, Honda Y, Kuroda S, Sekigawa M, Kanoh M, Suzuki M, Inaba O, Muramatsu
K, Yamato T, Matsumura Y, Asakawa K, Ebana Y, Furukawa T, Hirao K, Isobe M. Impact of SNP on
IL6R (rs7514452) for age at onset of atrial ﬁbrillation. The 78th Annual Scientiﬁc Meeting of the Japanese
Circulation Society Tokyo
18. Sekigawa M, Satoh A, Nitta J, Sato Y, Honda Y, Kuroda S, Kanoh M, Suzuki M, Inaba O, Muramatsu
K, Yamato T, Matsumura Y, Asakawa K, Ebana Y, Furukawa T, Hirao K, Isobe M. Eﬀect of SNP on
9q22 (rs6479562) on the progression from paroxysmal atrial ﬁbrillation to persistent atrial ﬁbrillation.
The 78th Annual Scientiﬁc Meeting of the Japanese Circulation Society Tokyo― 309 ―

Systemic Organ Regulation

Systemic Organ Regulation

Stem Cell Regulation

Professor Tetsuya TAGA
Associate Professor Ikuo NOBUHISAAssistant Professor Kouichi TABUTechnical Assistant/Administrative Assistant Kazuko INOUE(1)OutlineResearch in this department has been conducted to elucidate the mechanisms by which stem cells are regulated.
The major focus has been on neural stem cells, hematopoietic stem cells, and cancer stem cells. The study
is aimed to understand development, maintenance, and regeneration of the central nervous system and thehematopoietic system, and to obtain a clue to tackle the problem of cancer recurrence. Particular attention is
given to cell-external cues (such as cytokines) and cell-intrinsic programs (including epigenetic modiﬁcation),taking cross-interactions of transcriptional regulatory signals into consideration.
(2)

ResearchResearch Subjects in this department are as follows:
1) Molecular basis for the maintenance of neural stem cells
2) Regulation of the neural stem cell fate
3) Characterization of hematopoietic stem cells in fetal hematopoietic organs
4) Characterization of cancer stem cells and their niche
5) Epigenetic regulation of neural development(3)

Education

Our education has been conducted to elucidate the mechanisms by which stem cells are regulated. The major
focus has been on neural stem cells, hematopoietic stem cells, and cancer stem cells. The study is aimed tounderstand development, maintenance, and regeneration of the central nervous system and the hematopoietic
system, and to obtain a clue to tackle the problem of cancer recurrence. The projects have been performed, forinstance by elucidation of stem cell characteristics, analysis of transcriptional regulatory signaling pathways,
and identiﬁcation of niche signals.
(4)

Lectures & Courses

Under our education program, students will learn the molecular basis of stem cell regulation in view of cellextrinsic signals and cell intrinsic-programs during tissue development, maintenance, and regeneration frommolecular to whole-body levels. Students will receive exposure to cutting edge concepts and research technologies, and study regulatory mechanisms in neural, hematopoietic, and cancer stem cells. With emphasis
also on physiological and pathological conditions surrounding the stem cells, we aims to improve student’s
understanding of stem cells from multiple viewpoints.

― 310 ―

Systemic Organ Regulation

Systemic Organ Regulation(5)

Publications[Original Articles]
1. Inagaki T, Kusunoki S, Tabu K, Okabe H, Yamada I, Taga T, Matsumoto A, Makino S, Takeda S, andKato K. Up-regulation of lymphocyte antigen 6 complex expression in side-population cells derived froma human trophoblast cell line HTR-8/SVneo. Hum Cell. 2016.01; 29; 10-21
2. Sudo G, Kagawa T, Kokubu Y, Inazawa J, and Taga T. Increase of GFAP-positive astrocytes in histone
demethylase GASC1/KDM4C/JMJD2C hypomorphic mutant mice. Genes Cells. 2016.03; 21; 218-225
3. Kokubu Y, Tabu K, Wang W, Muramatsu N, Murota Y, Nobuhisa I, Jinushi M, and Taga T. Induction of
protumoral CD11chigh macrophages by glioma cancer stem cells through GM-CSF. Genes Cells. 2016.03;
21; 241-251
4. Tabu K, Muramatsu N, Mangani C, Wu M, Zhang R, Kimura T, Terashima K, Bizen N, Kimura R, Wang
W, Murota Y, Kokubu Y, Nobuhisa I, Kagawa T, Kitabayashi I, Bradley M, and Taga T. A synthetic
polymer scaﬀold reveals the self-maintenance strategies of rat glioma stem cells by organization of theadvantageous niche. Stem Cells. 2016.05; 34; 1151-1162
5. Kimura T, Wang L, Tabu K, Tsuda M, Tanino M, Maekawa A, Nishihara H, Hiraga H, Taga T, Oda Y,
and Tanaka S. Identiﬁcation and analysis of CXCR4-positive synovial sarcoma-initiating cells. Oncogene.
2016.07; 35; 3932-3943
6. Murota Y, Tabu K, and Taga T. Requirement of ABC transporter inhibition and Hoechst 33342 dye
deprivation for the assessment of side population-deﬁned C6 glioma stem cell metabolism using ﬂuorescentprobes. BMC Cancer. 2016.11; 16; 847
7. Wang W, Tabu K, Hagiya Y, Sugiyama Y, Kokubu Y, Murota Y, Ogura SI, and Taga T. Enhancementof 5-aminolevulinic acid-based ﬂuorescence detection of side population-deﬁned glioma stem cells by ironchelation. Sci Rep. 2016.12; In press[Conference Activities & Talks]
1. Sudo G. Analysis of pathogenic mechanisms of neurodevelopmental disorder-like phenotype exhibited byhistone demethylase Gasc1 hypomorphic mutant mice. The 9th Annual Meeting for Japanese Developmental Neuroscientists 2016.03.18 Tokyo Medical and Dental University, Tokyo
2. Saito K, Nobuhisa I, Anani M, Harada K, Takahashi S, and Taga T. Mechanism of maintaining hematopoietic stem and progenitor cell phenotype of intra-aortic cell clusters in the AGM region through the Sox17Notch1-Hes1 axis. The 14th Stem Cell Symposium 2016.05.20 Awaji Yumebutai International ConferenceCenter, Hyogo, Japan
3. Tabu K, Kokubu Y, Muramatsu N, Nomoto S, Wang W and Taga T. Adaptive response of rat C6 glioma
stem cells to iron deprivation by which the development of tumor inﬁltrating macrophages is induced.
The 14th Stem Cell Research Symposium 2016.05.20 Awaji Yumebutai International Conference Center,
Awaji, Japan
4. Wang W, Tabu K, Sugiyama Y, Hagiya Y, Ogura S and Taga T. Resistance of glioma stem cells to 5aminolevulinic acid (ALA)-based detection due to enhanced metabolic conversion of protoporphyrin IX.
The 14th Stem Cell Research Symposium 2016.05.20 Awaji Yumebutai International Conference Center,
Awaji, Japan
5. Murota Y, Tabu K and Taga T. C6 glioma stem cell-derived extracellular vesicles promote the developmentof macrophages. The 14th Stem Cell Research Symposium 2016.05.20 Awaji Yumebutai International
Conference Center, Awaji, Japan
6. Tabu K, Wang W, Murota Y and Taga T. Adaptive response of C6 glioma stem cells to iron deprivation
through macrophage induction. The 75th Annual Meeting of the Japanese Cancer Association 2016.10.06
Paciﬁco Yokohama, Yokohama, Japan
7. Wang W, Tabu K, Hagiya Y, Murota Y, Ogura S and Taga T. Enhancement of 5-aminolevulinic acid-based
ﬂuorescence detection of glioma stem cells by chelating iron. The 75th Annual Meeting of the JapaneseCancer Association 2016.10.06 Paciﬁco Yokohama, Yokohama, Japan― 311 ―

Systemic Organ Regulation

Systemic Organ Regulation

8. Saito K, Nobuhisa I, Anani M, Harada K, Takahashi S, Lickert H, Kanai M, Kanai Y, and Taga T.
Maintenance of hematopoietic stem and progenitor cell phenotype of intra-aortic cell clusters in the AGM
region through the Sox17-Notch1-Hes1 axis. The 39th Annual Meeting of the Molecular Biology Societyof Japan 2016.12.01 Paciﬁco Yokohama, Yokohama, Japan
9. Takahashi S, Nobuhisa I, Saito K, and Taga T. Involvement of transcription factor Sox17-mediated expression of adhesion molecules in hematopoietic cell cluster formation in midgestation mouse dorsal aorta. The
39th Annual Meeting of the Molecular Biology Society of Japan 2016.12.01 Paciﬁco Yokohama, Yokohama,Japan
10. Kagawa T, Yamaguchi Y, Sudo G, Kokubu Y, Hattori S, Takao K, Miyakawa T, Inazawa J, and Taga T.
Astroglial development is regulated by DNA and histone methylation: from molecular basis to behavioral
abnormalities in gene-manipulated mice. Cold Spring Harbor Asia meeting on Novel Insights into Glia
Function & Dysfunction 2016.12.06 Suzhou Industrial Park Conference Center, Suzhou, China― 312 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

Molecular Pharmacology

Professor: Masaki Noda, M.D., Ph.D.
Associate Professor: Yoichi Ezura, M.D., Ph.D.
Assistant Professor: Yayoi Izu, DVM, Ph.D.
Research Assistant Professor: Smriti Aryal A.C., DDS, Ph.D.
(1)

OutlineIn order to contribute to the establishment of therapy and prevention for osteoporosis and the other calciumrelated disorders, we are elucidating molecular mechanisms underlying regulation of calcium metabolism withemphases on bone formation and resorption. Skeletal system is the largest storage site for calcium in a living
body and its metabolism is conducted by a complex cell society consisting of bone-forming osteoblasts andbone-resorbing osteoclasts as well as stromal cells and chondrocytes. In our department, we take molecular andcellular biological approaches to study the mechanisms underlying regulation of development, diﬀerentiation,and function of these cells.
(2)

Research

Bone is the major organ for calcium metabolism in our body. The regulation of bone metabolism is mediated by abalance between osteoblastic bone formation and osteoclastic bone resorption. These activities are maintained inbalance and called bone remodeling. Imbalance of the remodeling results in development of bone disorders, suchas osteoporosis. Osteoblasts are diﬀerentiated from mesenchymal stem cells. These cells are under the regulation
by local and systematical factors, such as growth factors and hormones. These factors activate intracellularsignaling, which promotes transcription factors thereby delineates cell diﬀerentiation. In our laboratory, we are
studying the process from various aspects of bone cell regulation including transcription factors, cytokines andhormones. To promote our study, knockout and transgenic mice, gene introduction via virus, global analysis
of gene expression, and genome database analysis are used. Our study will provide the basic understandingsof bone homeostasis, which will contribute the development of measures for diagnosis and treatment of bonedisorders.
(3)

Publications[Original Articles]
1. Sakie Katsumura, Yoichi Ezura, Yayoi Izu, Jumpei Shirakawa, Atsushi Miyawaki, Kiyoshi Harada, Masaki
Noda. Beta Adrenergic Receptor Stimulation Suppresses Cell Migration in Association with Cell Cycle
Transition in Osteoblasts-Live Imaging Analyses Based on FUCCI System. J. Cell. Physiol.. 2016.02;
231(2); 496-504
2. Katsumura S, Ezura Y, Izu Y, Shirakawa J, Miyawaki A, Harada K, Noda M. Beta Adrenergic Receptor Stimulation Suppresses Cell Migration in Association with Cell Cycle Transition in Osteoblasts-Live
Imaging Analyses Based on FUCCI System J Cell Physiol. 2016.02; 231(2); 496-504
3. Lin W, Ezura Y, Izu Y, Aryal SA, Kawasaki M, Chantida PN, Moriyama K, Noda M. Proﬁlin Expression Is
Regulated by Bone Morphogenetic Protein (BMP) in Osteoblastic Cells. Journal of Cellular Biochemistry.
2016.03; 117(3); 621-628― 313 ―

Systemic Organ
Organ Regulation
Regulation
Systemic

4. Shirakawa J, Harada H, Noda M, Ezura Y. PTH-Induced Osteoblast Proliferation Requires Upregulationof the Ubiquitin-Speciﬁc Peptidase 2 (Usp2) Expression Calcif Tissue Int. 2016.03; 98(3); 306-315
5. Lin W, Ezura Y, Izu Y, Aryal SA, Kawasaki M, Chantida PN, Moriyama K, Noda M. Proﬁlin Expression
Is Regulated by Bone Morphogenetic Protein (BMP) in Osteoblastic Cells J Cell Biochem. 2016.03;
117(3); 621-628― 314 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

Stem Cell Biology

Professor : Emi Nishimura, M.D., Ph.D.
Associate Professor: Daisuke Nanba, Ph.D
Assistant Professor : Hiroyuki Matsumura, Ph.D.
Project Assistant Professor :Yasuaki Mohri, Ph.D.
Makoto Fukuda, Ph.D.
JSPS Research Fellow : Hironobu Morinaga, Ph.D.
(1)

OutlineStem cell systems play fundamental roles in tissue turnover and homeostasis. Our goal is to understand themechanisms of tissue homeostasis driven by stem cell systems and to apply the knowledge to better understandthe mechanisms underlying speciﬁc tissue decline, cancer development and other diseases associated with aging.
We further aim to apply this knowledge to regenerative medicine using somatic stem cells and the treatment ofcancer as well as other age-associated diseases.
(2)

Research

1) Identiﬁcation of stem cells in the skin
The skin is the largest organ in the body. Hair follicles in the skin constantly renew themselves by alternate
phases of growth, regression and rest. During this process, mature melanocytes (pigment cells) in hair follicles
are replaced by a new cell population every hair cycle. We previously identiﬁed the source of those melanocytes,
melanocyte stem cells (McSC), which are located in the hair follicle bulge and supply mature melanocytes
required for hair and skin pigmentation (Nishimura EK et al. Nature 2002). We currently identiﬁed McSCs ineccrine sweat glands in non-hair-bearing skin areas as well. Also we are currently searching for the prospective
method for identiﬁcation of epidermal keratinocyte stem cells in mouse and human skin.
2) Mechanisms of stem cell maintenance
The underlying mechanisms of stem cell maintenance is a fundamental issue in stem cell biology and medicine.
We previously demonstrated that the niche microenvironment plays dominant role in melanocyte stem cellfate determination (Nishimura EK et al. 2002). We then revealed that hair follicle stem cells (HFSC), whichsurround McSCs in the hair follicle bulge-subbulge area, serve as a functional niche for McSC maintenancethrough transforming growth factor β (TGF-β)(Nishimura EK et al. Cell Stem Cell, 2010)(Tanimura S et al.
Cell Stem Cell 2011). As intrinsic defects in stem cells such as caused by Mitf or Bcl2 deﬁciency also induces
McSC depletion which leads to the progressive expression of hair graying phenotype, incomplete maintenanceof McSCs either by defective signaling from the stem cell niche or by intrinsic defects in stem cells induces the
progressive hair graying phenotype.
3) Mechanisms for stem cell aging and quality control of stem cell pools.
Physiological hair graying is the most obvious outward sign of aging in mammals, yet it has been unclear whatcauses the incomplete maintenance of MsSCs during the course of aging (Nishimura EK et al. Science 2005).
We have found that genotoxic stress abrogates renewal of McSCs by triggering their diﬀerentiation without
inducingstem cell apoptosis nor cellular senescence. Our ﬁndings indicated that a stem cell renewal checkpoint exists to maintain the quality of the melanocyte stem cell pool (Inomata K, Aoto T et al. Cell 2009).
Interestingly, a similar mechanism actually underlies epithelial tissue aging (See Highlight).

― 315 ―

Systemic Organ
Organ Regulation
Regulation
Systemic

4) Development of skin regeneration technology with human skin stem cells
Human epidermal keratinocyte stem cells can be cultivated under suitable conditions, and generate a progeny
large enough to entirely reconstitute the epidermis of an adult human. This has enabled the autologous transplantation of cultured epidermal sheets onto patients with extensive burns. However, the cultured keratinocytes
can regenerate only the epidermis and cannot suppress dermal scarring. To develop novel skin regeneration technology, we have investigated human epidermal keratinocytes and dermal ﬁbroblasts, and obtained the following
results. 1) Human epidermal keratinocyte stem cells can be identiﬁed in situ by analyzing cell motion during
their cultivation (Nanba et al., J. Cell Biol., 2015, Tate et al., J. Dermatol. Sci. 2015). The identiﬁcation of
keratinocyte stem cells by image analysis is a valid parameter for quality control of cultured keratinocytes fortransplantation, and improves the clinical outcome of cell therapy and the eﬃciency of cell manufacturing forregenerative medicine. 2) Human dermal ﬁbroblasts can be categorized at least two functional clonal types bycomprehensive phenotypic and gene expression proﬁling (Hiraoka et al., J. Dermatol. Sci. in press). One ishighly proliferative, while the other is less proliferative but has the ability to remodel the tissue architecture.
The proliferative clones are predominant in infants, but decrease with physiological aging. These data have
implications regarding the functional heterogeneity of dermal ﬁbroblasts and skin repair and aging.

(3)

Publications[Original Articles]
1. Hiroyuki Matsumura,* Yasuaki Mohri,* Nguyen Thanh Binh,* Hironobu Morinaga, Makoto Fukuda,
Mayumi Ito, Sotaro Kurata, Jan Hoeijmakers, Emi K. Nishimura. Hair follicle aging is driven by transepidermal elimination of stem cells via COL17A1 proteolysis Science. 2016.02; 351(6273); 575-589
2. Karigane D, Kobayashi H, Morikawa T, Ootomo Y, Sakaki M, Nagamatsu G, Kubota Y, Goda N, Matsumoto M, Nishimura EK, Soga T, Otsu K, Suematsu M, Okamoto S, Suda T, and Takubo K. P38
α Activates Purine Metabolism to Initiate Hematopoietic Stem/Progenitor Cell Cycling in Response to
Stress Cell Stem Cell. 2016.08; 19(2); 192-204[Conference Activities & Talks]
1. Emi Nishimura. Regulation of Tissue Stem Cells in Hair Follicle Regulation and Aging. The 89th Annual
Meeting of the Japanese Pharmacological Society 2016.03.09
2. Emi Nishimura. stem cell aging: a clue to understand hair thinning and graying. 1st International
Symposium on Stem Cell Aging and Disease 2016.06.29 Tokyo, Japan
3. Emi Nishimura. Mechanisms of hair follicle aging and hair loss. Cell Symposia: Aging and Metabolism
2016 2016.07.10 Melia Sitges, Spain
4. Emi Nishimura. New Insights in Stem Cell Research & Age-related Changes in Skin and Hair Pigmentation. 46th Annual ESDR Meeting 2016.09 Munich, Germany
5. Emi Nishimura. Melanocyte Stem Cells in eccrine seat glands: a potential of acral melanoma. 12th
Meeting od the German-Japanese Society of Dermatology 2016.10.13 Nagano, Japan― 316 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

Respiratory Medicine

Professor: Naohiko INASE
Junior Associate Professor: Kimitake TSUCHIYA
Assistant Professor: Toshihide FUJIE, Tomoya TATEISHI, Haruhiko FURUSAWA, Masahiro ISHIZUKA,
Graduate Students: Mayuko TAO, Sahoko CHIBA, Yumi SAKAKIBARA,
Masahiro MASUO, Tsuyoshi SHIRAI, Makiko SUGIURA, Yuta ADACHI, Ken UCHIBORI,
Yu KUSAKA, Rie SAKAKIBARA, Manabu SEMA, Tomoko TERADA, Yoshihisa NUKUI, Takayuki HONDA,
Takahiro MITSUMURA, Sho SHIBATA, Yukihisa INOUE, Rie KURASHIGE, Hiroaki SAITOH, Satoshi HANZAWA(1)OutlineRespiratory Medicine deals with a variety of pulmonary diseases including tumors, infectious diseases, allergic
diseases, non-allergic inﬂammatory diseases, and genetic disorders.

(2)

Research

1) Pathogenesis of hypersensitivity pneumonitis and detection of environmental causative antign
2) Airway remodeling in bronchial asthma model3) Acute exacerbation in pulmonary ﬁbrosis
4) Proteomics of pulmonary ﬁbrosis5) Pathogenesis of pulmonary ﬁbrosis and emphysema(3)

Education

Main objective in the graduate course is to provide students to study speciﬁc diagnostic modalities as well asbasic scientiﬁc ﬁndings regarding the pathogenesis of pulmonary diseases. Students are also taught on basic
science and its related laboratory technology depending upon their research subject.

(4)

Lectures & Courses

Students should try to understand a variety of pulmonary diseases in terms of scientiﬁc aspect and make anappropriate plan to examine unsolved research questions.

(5)

Clinical Services & Other Works

Our clinic provides a full spectrum of diagnosis and treatment of a variety of pulmonary diseases. Consultant
system is open to all departments in this hospital and daily clinical conference regarding inpatients is organizedby professors of the department. In outpatient clinic, chemotherapy, home oxygen therapy, management of sleep
apnea, and arrange of clinical studies are provided.

― 317 ―

Systemic Organ
Organ Regulation
Regulation
Systemic(6)

Clinical Performances

We have immunological tools to examine hypersensitivity pneumonitis including antigen inhalation challenge
test, speciﬁc antibody against causative antigen, and lymphocyte proliferation test. Many patients with interstitial lug diseases in Japan are referred to our clinic.
(7)

Publications[Original Articles]
1. Chiba S, Tsuchiya K, Akashi T, Ishizuka M, Okamoto T, Furusawa H, Tateishi T, Kishino M, Miyazaki Y,
Tateishi U, Takemura T, Inase N. Chronic hypersensitivity pneumonitis with a usual interstitial pneumonia(UIP)-like pattern: Correlation between histopathological and clinical ﬁndings. CHEST. 2016; 149; 14731481
2. Sakakibara Y, Ohtani Y, Jinta T, Fujie T, Miyazaki Y, Inase N, Saito R, Akaza M, Sasano T, Sumi
Y. Concentration of immunoglobulin G antibodies against pertussis toxin does not decrease over a long
period of time in Japan. Internal Medicine. 2016; 55; 3257-3263
3. Masuo M, Miyazaki Y, Suhara K, Ishizuka M, Fujie T, Inase N. Factors associated with positive inhalation
provocation test results in subjects suspected of having chronic bird-related hypersensitivity pneumonitis.
Respir Investig. 2016; 54; 454-461
4. Suhara K, Miyazaki Y, Okamoto T, Ishizuka M, Tsuchiya K, Inase N. Fragmented gelsolins are increased inrheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern. Allergol
Int. 2016.01; 65(1); 88-95[Misc]
1. Miyazaki Y, Tsutsui T, Inase N. Treatment and monitoring of hypersensitivity pneumonitis. Expert Rev
Clin Immunol.. 2016.09; 12(9); 953-962[Conference Activities & Talks]
1. Nukui, Y, Miyazaki Y, Inase N. Proteomics analysis of BALF in sarcoidosis associated pulmonary ﬁbrosis.
ATS International Conference 2016.05.15 San Francisco
2. Tateishi, Fujie T, Miyazaki Y, Inase N. Clinical characteristics of community-acquired pneumonia in thepatients with pulmonary ﬁbrosis. ATS International Conference 2016.05.15 San Francisco
3. Sema M, Sakashita H, Miyazaki Y, Inase N. Clinical characteristics of lung cancer diagnosed duringfollow-up of interstitial pneumonia. ATS International Conference 2016.05.16 San Francisco
4. Wakabayashi K, Adachi Y, Tateishi T, Inase N, Nakazawa K. Ventilator type and setting for patients withacute respiratory failure on chronic interstitial lung disease. ATS International Conference 2016.05.17 San
Francisco
5. Masuo M, Miyazaki Y, Fujie T, Inase N, Nakamura M, Ohbo K. Human epididymis protein 4 is a novel
biomarker for chronic ﬁbrosing interstitial pneumonias. ATS International Conference 2016.05.17 San
Francisco
6. Furusawa H, Sugiura M, Miyazaki Y, Inase N. The usefulness of direct hemoperfusion with polymyxin
B-immobilized ﬁber (PMX-DHP) treatment on acute exacerbation of interstitial pneumonitis. ATS International Conference 2016.05.17 San Francisco
7. Jin Y,Shimada S,Yamauchi S,Matsubara O,Yamanaka K,Inase N. Myeloid-derived suppressor cell expression within the microenvironment of lung adenocarcinoma.. IASLC 17th World Conference of Lung
Cancer, Vienna 2016.11.04 AUSTRIA― 318 ―

Systemic Organ Regulation

Systemic Organ Regulation

Gastroenterology and Hepatology
Professor

Mamoru WATANABE

Professor

Yasuhiro ASAHINA (Department for Hepatitis Control)
Kazuo OHTSUKA (Department of Endoscopic Diagnosis and Therapeutics)
Ryuichi OKAMOTO (Center for Stem Cell and Regenerative Medicine)
Tetsuya NAKAMURA(Department of Advanced Therapeutics in Gastrointestinal Diseases)

Associate Professor
Kiichiro TSUCHIYA
Shinya OOKA (Cancer Center)
Mina NAKAGAWA (Center for Interprofessional Education)
Akihiro ARAKI (Center for Personalized Medicine for Healthy Aging)
Project Associate Professor
Masakazu NAGAHORI
Junior Associate Professor
Cheng-Hsin AZUMASei KAKINUMA (Department for Hepatitis Control),Yasuhiro ITSUI (Department of General Medicine)
Eriko OKADA (Department of Endoscopic Diagnosis and Therapeutics)
Katsuyoshi MATSUOKA(Department of Advanced Therapeutics in Gastrointestinal Diseases)
Assistant Professor
Shigeru OSHIMA, Takashi NAGAISHI, Toshimitsu FUJII, Yasuhiro NEMOTO
Tomohiro MIZUTANI(Department of Advanced Therapeutics in Gastrointestinal Diseases)
Michio ONIZAWA(Department of Advanced Therapeutics in Gastrointestinal Diseases)
Masayoshi FUKUDA(Department of Endoscopic Diagnosis and Therapeutics)
Miyako MURAKAWA(Clinical Laboratory)
Project Assistant Professor
Sayuri NITTA, Ryu NISHIMURA
Hospital Staﬀ

AMED Fellow
Fellow

Isamu SHIBATA, Kenichiro KOHASHI, Sachi SAKURAI, Kengo NOZAKI, Kenji OTANI,
Tatsuo MURANO, Taichi MATSUMOTO, Masanori KOBAYASHI, Toru NAKATA,
Yoichi NIBE, Masato YAUCHI, Konomi KUWAHARA, Maiko KIMURA,
Fukiko KITAHATA, Kento TAKENAKA, Nobuhiro KATSUKURA
Keita FUKUSHIMA, Syuji HIBIYA,
Satoru FUjii

Graduate Student
Fumio GOTO, Taichi MATSUMOTO, Masanori KOBAYASHI, Toru NAKATA,
Youichi NIBE, Satoru FUJII, Kento TAKENAKA, Hiroko NAGATA,
Shun KANEKO, Chiaki MAEYASHIKI, Taro WATABE, Yuka MATSUMOTO,
Kohei SUZUKI, Akinori HOSOYA, Yu ASANO, Emi INOUE, Shintaro AKIYAMA,
Fumiaki ISHIBASHI, Ami KAWAMOTO, Masato MIYOSHI, Tomoyuki TSUNODA,
Yuria TAKEI, JOSE Nisha, Sho ANZAI, Tomoaki SHIRASAKI, Shohei TANAKA,
Reiko KUNO, Kana OTSUBO, Ayako Sato, Sho WARANABE, JOSE Nisha,― 319 ―
Arisa TOUKAISystemic Organ Regulation(1)

Systemic Organ Regulation

OutlineResearch project is selected from the clinical problems in the Gastroenterology and Hepatology to understandthe research policy, as clinical science that the results of research project ﬁnally should be restored to clinicalmedicine.
The purpose of this course is the understanding the situation of inﬂammatory bowel disease (IBD) in Japanand the problems about the pathogenesis and intractable cause of IBD. In addition, the understanding thepatogeneisis and problems about the liver diseases such as viral hepatitis, cirrhosis and hepatocelluar carcinoma
is the purpose of this course.

(2)

Research

Basic Research Projects
Systemic Organ Regulation· Elucidating the pathophysiology of inﬂammatory bowel diseases and development of treatment by diseasespeciﬁc
immue-regulation.
· Development of novel therapeutics for inﬂammatory and allergic diseases based on gut–speciﬁc mucosal immune
regulation.
· Basic research and clinical application of regenerative medicine in gastrointestinal diseases.
· Analysis of interferon-resistant hepatitis C virus.
· Comprehensive analysis of susceptibility genes for various gastrointestinal diseases.
· Crosstalk of the signaling pathways in intestinal epithelial cells.
· Functional analysis of the intestine using primary cell curture in vitro.
(3)

Education

We believe that the central role of clinical departments in the graduate school is to establish basis for the
innovative medicine / medical treatment in the next generation. Basic research lead by clinical concepts, anddevelopment of novel therapeutics established upon basic research are both critically required to achieve our
mission. Therefore, our primary goal is set to train highly educated and experienced clinician-researchers in theﬁeld of gastroenterology and hepatology.
In the clinical area, we pursue development and application of highly advanced technologies, including novel
endoscopic procedures, for sophisticated diagnosis and treatment of gastrointestinal and liver diseases. In basic
research, our principle is to achieve "clinical science", a research evoked from various clinical problems, and also
directed to launch innovative therapeutic procedures to the daily clinical practice. Based on these principals, weare running research projects to 1) develop novel therapy for refractory inﬂammatory bowel diseases, 2) prevent
progression of liver failure in chronic hepatitis patients and 3) improve anti-cancer therapy for the treatment ofgastrointestinal malignancies, by expanding our distinct basic research ﬁndings in the area of mucosal immunology, liver immunology, regenerative medicine and virology, to various clinical settings. Moreover, we promote
both intra- and inter-national exchanges of researchers, and provide good opportunities to study abroad. The
ﬁnal goal of our education is to promote students to become a well-developed clinician researcher, and also a
leading expert in the ﬁeld of gastroenterology and hepatology.
(4)

Lectures & Courses

Research Conference
Journal Club(5)

every Tuesday 18:00 19:30
every Tuesday 18:00 19:30

Clinical Services & Other Works

Expert Areas in Clinical Practice· Immune-regulation based treatment of inﬂammatory bowel diseases.

― 320 ―

Systemic Organ Regulation

Systemic Organ Regulation· Prevention of chronic hepatitis progression to hepatocellular cancer and liver failure, by virology-based
treatment strategy.
· Clinical trial of innovative treatment for hepatocellular cancer.
· Diagnosis and treatment of small intestinal diseases by balloon enteroscopy and capsule enteroscopy.
· Advanced diagnosis and treatment of colonic diseases by colonoscopy.
· Development of minimally-invasive diagnostic modalities for gastrointestinal diseases (i.e. MR enteroclysis).
· Improved chemotherapy for gastric and pancreatic malignancies.
(6)

Clinical Performances

Therapeutics of inﬂammatory bowel diseases by corrections of immunological disfuctions.
Diagnostic and interventional gastrointestinal endoscopy
Antiviral therapies against chronic viral hepatitis and preventions of hepatic malignancy novel intervensions ofhepatic malignancy.
(7)

Publications[Original Articles]
1. Jun Itakura, Masayuki Kurosaki, Chitomi Hasebe, Yukio Osaki, Kouji Joko, Hitoshi Yagisawa, Shinya
Sakita, Hiroaki Okushin, Takashi Satou, Hiroyuki Hisai, Takehiko Abe, Keiji Tsuji, Takashi Tamada,Haruhiko Kobashi, Akeri Mitsuda, Yasushi Ide, Chikara Ogawa, Syotaro Tsuruta, Kouichi Takaguchi,
Miyako Murakawa, Yasuhiro Asahina, Nobuyuki Enomoto, Namiki Izumi. Complex Pattern of ResistanceAssociated Substitutions of Hepatitis C Virus after Daclatasvir/Asunaprevir Treatment Failure. PLoS
ONE. 2016; 11(10); e0165339
2. Takashi Nagaishi, Taro Watabe, Nisha Jose, Arisa Tokai, Toshimitsu Fujii, Katsuyoshi Matsuoka, Masakazu
Nagahori, Kazuo Ohtsuka, Mamoru Watanabe. Epithelial Nuclear Factor-κ B Activation in Inﬂammatory
Bowel Diseases and Colitis-Associated Carcinogenesis Digestion. 2016.01; 93(1); 40-46
3. Yasuhiro Asahina, Mamoru Watanabe. Need for appropriate programs for prevention of hepatitis B
infection: Lessons from the latest nationwide survey in Japan. J. Gastroenterol. Hepatol. 2016.01; 31(1);12-13
4. Satoshi Otani, Sei Kakinuma, Akihide Kamiya, Fumio Goto, Shun Kaneko, Masato Miyoshi, Tomoyuki
Tsunoda, Yu Asano, Fukiko Kawai-Kitahata, Sayuri Nitta, Toru Nakata, Ryuichi Okamoto, Yasuhiro Itsui,
Mina Nakagawa, Seishin Azuma, Yasuhiro Asahina, Tomoyuki Yamaguchi, Naohiko Koshikawa, Motoharu
Seiki, Hiromitsu Nakauchi, Mamoru Watanabe. Matrix metalloproteinase-14 mediates formation of bileducts and hepatic maturation of fetal hepatic progenitor cells. Biochem. Biophys. Res. Commun.
2016.01; 469(4); 1062-1068
5. Tetsuhide Ito, Hiroshi Ishiguro, Hirotaka Ohara, Terumi Kamisawa, Junichi Sakagami, Naohiro Sata,
Yoshifumi Takeyama, Morihisa Hirota, Hiroyuki Miyakawa, Hisato Igarashi, Lingaku Lee, Takashi Fujiyama, Masayuki Hijioka, Keijiro Ueda, Yuichi Tachibana, Yoshio Sogame, Hiroaki Yasuda, Ryusuke
Kato, Keisho Kataoka, Keiko Shiratori, Masanori Sugiyama, Kazuichi Okazaki, Shigeyuki Kawa, Yusuke
Tando, Yoshikazu Kinoshita, Mamoru Watanabe, Tooru Shimosegawa. Evidence-based clinical practiceguidelines for chronic pancreatitis 2015. J. Gastroenterol. 2016.02; 51(2); 85-92
6. Yasuhiro Asahina, Namiki Izumi, Kumada Hiromitsu, Masayuki Kurosaki, Kazuhiko Koike, Fumitaka
Suzuki, Hajime Takikawa, Atsushi Tanaka, Eiji Tanaka, Yasuhito Tanaka, Hirohito Tsubouchi, Norio
Hayashi, Naoki Hiramatsu, Hiroshi Yotsuyanagi. JSH Guidelines for the Management of Hepatitis C
Virus Infection: A 2016 update for genotype 1 and 2. Hepatol. Res. 2016.02; 46(2); 129-165
7. Fukiko Kawai-Kitahata, Yasuhiro Asahina, Shinji Tanaka, Sei Kakinuma, Miyako Murakawa, Sayuri Nitta,
Takako Watanabe, Satoshi Otani, Miki Taniguchi, Fumio Goto, Hiroko Nagata, Shun Kaneko, Megumi
Tasaka-Fujita, Yuki Nishimura-Sakurai, Seishin Azuma, Yasuhiro Itsui, Mina Nakagawa, Minoru Tanabe,
Shinichi Takano, Mitsuharu Fukasawa, Minoru Sakamoto, Shinya Maekawa, Nobuyuki Enomoto, Mamoru
Watanabe. Comprehensive analyses of mutations and hepatitis B virus integration in hepatocellular
carcinoma with clinicopathological features. J. Gastroenterol. 2016.03; 51(5); 473-486― 321 ―

Systemic Organ Regulation

Systemic Organ Regulation

8. Taku Kobayashi, Yasuo Suzuki, Satoshi Motoya, Fumihito Hirai, Haruhiko Ogata, Hiroaki Ito, Noriko
Sato, Kunihiko Ozaki, Mamoru Watanabe, Toshifumi Hibi. First trough level of inﬂiximab at week 2
predicts future outcomes of induction therapy in ulcerative colitis-results from a multicenter prospective
randomized controlled trial and its post hoc analysis. J. Gastroenterol. 2016.03; 51(3); 241-251
9. Kengo Nozaki, Wakana Mochizuki, Yuka Matsumoto, Taichi Matsumoto, Masayoshi Fukuda, Tomohiro
Mizutani, Mamoru Watanabe, Tetsuya Nakamura. Co-culture with intestinal epithelial organoids allows
eﬃcient expansion and motility analysis of intraepithelial lymphocytes. J. Gastroenterol. 2016.03; 51(3);206-213
10. Miki Taniguchi, Megumi Tasaka-Fujita, Mina Nakagawa, Takako Watanabe, Fukiko Kawai-Kitahata,
Satoshi Otani, Fumio Goto, Hiroko Nagata, Shun Kaneko, Sayuri Nitta, Miyako Murakawa, Yuki NishimuraSakurai, Seishin Azuma, Yasuhiro Itsui, Kenichi Mori, Shintaro Yagi, Sei Kakinuma, Yasuhiro Asahina,
Mamoru Watanabe. Evaluation of Interferon Resistance in Newly Established Genotype 1b Hepatitis C
Virus Cell Culture System. J Clin Transl Hepatol. 2016.03; 4(1); 5-11
11. Seishin Azuma, Yasuhiro Asahina, Yuki Nishimura-Sakurai, Sei Kakinuma, Shun Kaneko, Hiroko Nagata,
Fumio Goto, Satoshi Ootani, Fukiko Kawai-Kitahata, Miki Taniguchi, Miyako Murakawa, Takako Watanabe, Megumi Tasaka-Fujita, Yasuhiro Itsui, Mina Nakagawa, Mamoru Watanabe. Eﬃcacy of additional
radiofrequency ablation after transcatheter arterial chemoembolization for intermediate hepatocellular
carcinoma. Hepatol Res. 2016.04; 46(4); 312-319
12. Kazuo Ohtsuka, Kento Takenaka, Yoshio Kitazume, Toshimitsu Fujii, Katsuyoshi Matsuoka, Maiko
Kimura, Takashi Nagaishi, Mamoru Watanabe. Magnetic resonance enterography for the evaluationof the deep small intestine in Crohn’s disease. Intest Res. 2016.04; 14(2); 120-126
13. Shunsuke Komoto, Satoshi Motoya, Yuji Nishiwaki, Toshiyuki Matsui, Reiko Kunisaki, Katsuyoshi Matsuoka, Naoki Yoshimura, Takashi Kagaya, Makoto Naganuma, Nobuyuki Hida, Mamoru Watanabe, Toshifumi Hibi, Yasuo Suzuki, Soichiro Miura, Ryota Hokari, . Pregnancy outcome in women with inﬂammatory
bowel disease treated with anti-tumor necrosis factor and/or thiopurine therapy: a multicenter study fromJapan Intest Res. 2016.04; 14(2); 139-145
14. Ryuichi Okamoto, Mamoru Watanabe. Investigating cell therapy for inﬂammatory bowel disease. Expert
Opin Biol Ther. 2016.04; 1-9
15. Ryohei Hayashi, Kiichiro Tsuchiya, Keita Fukushima, Nobukatsu Horita, Shuji Hibiya, Keisuke Kitagaki,
Mariko Negi, Eisaku Itoh, Takumi Akashi, Yoshinobu Eishi, Eriko Okada, Akihiro Araki, Kazuo Ohtsuka,
Shinji Fukuda, Hiroshi Ohno, Ryuichi Okamoto, Tetsuya Nakamura, Shinji Tanaka, Kazuaki Chayama,
Mamoru Watanabe. Reduced Human α-defensin 6 in Noninﬂamed Jejunal Tissue of Patients with Crohn’s
Disease. Inﬂamm. Bowel Dis. 2016.05; 22(5); 1119-1128
16. Emma Calabrese, Christian Maaser, Francesca Zorzi, Klaus Kannengiesser, Stephen B Hanauer, David H
Bruining, Marietta Iacucci, Giovanni Maconi, Kerri L Novak, Remo Panaccione, Deike Strobel, Stephanie
R Wilson, Mamoru Watanabe, Francesco Pallone, Subrata Ghosh. Bowel Ultrasonography in the Management of Crohn’s Disease. A Review with Recommendations of an International Panel of Experts.
Inﬂamm. Bowel Dis. 2016.05; 22(5); 1168-1183
17. Kara G Lassen, Craig I McKenzie, Muriel Mari, Tatsuro Murano, Jakob Begun, Leigh A Baxt, Gautam
Goel, Eduardo J Villablanca, Szu-Yu Kuo, Hailiang Huang, Laurence Macia, Atul K Bhan, Marcel Batten,
Mark J Daly, Fulvio Reggiori, Charles R Mackay, Ramnik J Xavier. Genetic Coding Variant in GPR65
Alters Lysosomal pH and Links Lysosomal Dysfunction with Colitis Risk. Immunity. 2016.06; 44(6);1392-1405
18. Shun Kaneko, Sei Kakinuma, Yasuhiro Asahina, Akihide Kamiya, Masato Miyoshi, Tomoyuki Tsunoda,
Sayuri Nitta, Yu Asano, Hiroko Nagata, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako Murakawa, Yasuhiro Itsui, Mina Nakagawa, Seishin Azuma, Hiromitsu Nakauchi, Hironori Nishitsuji, Saneyuki Ujino,
Kunitada Shimotohno, Masashi Iwamoto, Koichi Watashi, Takaji Wakita, Mamoru Watanabe. Human
induced pluripotent stem cell-derived hepatic cell lines as a new model for host interaction with hepatitis
B virus. Sci Rep. 2016.07; 6; 29358
19. Hiroshi Fukui, Hidetsugu Saito, Yoshiyuki Ueno, Hirofumi Uto, Katsutoshi Obara, Isao Sakaida, Akitaka
Shibuya, Masataka Seike, Sumiko Nagoshi, Makoto Segawa, Hirohito Tsubouchi, Hisataka Moriwaki,― 322 ―

Systemic Organ Regulation

Systemic Organ Regulation

Akinobu Kato, Etsuko Hashimoto, Kojiro Michitaka, Toshikazu Murawaki, Kentaro Sugano, Mamoru
Watanabe, Tooru Shimosegawa. Evidence-based clinical practice guidelines for liver cirrhosis 2015. J.
Gastroenterol. 2016.07; 51(7); 629-650
20. Taichi Matsumoto, Wakana Mochizuki, Yoichi Nibe, Shintaro Akiyama, Yuka Matsumoto, Kengo Nozaki,
Masayoshi Fukuda, Ayumi Hayashi, Tomohiro Mizutani, Shigeru Oshima, Mamoru Watanabe, Tetsuya
Nakamura. Retinol Promotes In Vitro Growth of Proximal Colon Organoids through a Retinoic AcidIndependent Mechanism. PLoS ONE. 2016.08; 11(8); e0162049
21. Ryuichi Okamoto, Mariko Negi, Syohei Tomii, Yoshinobu Eishi, Mamoru Watanabe. Diagnosis andtreatment of microscopic colitis. Clin J Gastroenterol. 2016.08; 9(4); 169-174
22. Katsuhiko Iwakiri, Yoshikazu Kinoshita, Yasuki Habu, Tadayuki Oshima, Noriaki Manabe, Yasuhiro Fujiwara, Akihito Nagahara, Osamu Kawamura, Ryuichi Iwakiri, Soji Ozawa, Kiyoshi Ashida, Shuichi Ohara,
Hideyuki Kashiwagi, Kyoichi Adachi, Kazuhide Higuchi, Hiroto Miwa, Kazuma Fujimoto, Motoyasu Kusano, Yoshio Hoshihara, Tatsuyuki Kawano, Ken Haruma, Michio Hongo, Kentaro Sugano, Mamoru
Watanabe, Tooru Shimosegawa. Evidence-based clinical practice guidelines for gastroesophageal reﬂux
disease 2015. J. Gastroenterol. 2016.08; 51(8); 751-767
23. Naoki Hosoe, Kenji Watanabe, Takako Miyazaki, Masaaki Shimatani, Takahiro Wakamatsu, Kazuichi
Okazaki, Motohiro Esaki, Takayuki Matsumoto, Takayuki Abe, Takanori Kanai, Kazuo Ohtsuka, Mamoru
Watanabe, Keiichi Ikeda, Hisao Tajiri, Naoki Ohmiya, Masanao Nakamura, Hidemi Goto, Tomoyuki
Tsujikawa, Haruhiko Ogata. Evaluation of performance of the Omni mode for detecting video capsuleendoscopy images: A multicenter randomized controlled trial. Endosc Int Open. 2016.08; 4(8); E878-E882
24. Haruhiko Ogata, Mamoru Watanabe, Toshiyuki Matsui, Hidenori Hase, Motohiro Okayasu, Tsuyoshi
Tsuchiya, Yasuhiko Shinmura, Toshifumi Hibi. Safety of Adalimumab and Predictors of Adverse Eventsin 1693 Japanese Patients with Crohn’s Disease. J Crohns Colitis. 2016.09; 10(9); 1033-1041
25. Masato Miyoshi, Sei Kakinuma, Yoko Tanabe, Koji Ishii, Tian-Cheng Li, Takaji Wakita, Yukio Tsuura,
Hideki Watanabe, Yasuhiro Asahina, Mamoru Watanabe, Takaaki Ikeda. Chronic Hepatitis E Infection
in a Persistently Immunosuppressed Patient Unable to Be Eliminated after Ribavirin Therapy. Intern.
Med. 2016.10; 55(19); 2811-2817
26. Sayuri Nitta, Yasuhiro Asahina, Mami Matsuda, Norie Yamada, Ryuichi Sugiyama, Takahiro Masaki,
Ryosuke Suzuki, Nobuyuki Kato, Mamoru Watanabe, Takaji Wakita, Takanobu Kato. Eﬀects of ResistanceAssociated NS5A Mutations in Hepatitis C Virus on Viral Production and Susceptibility to Antiviral
Reagents. Sci Rep. 2016.10; 6; 34652
27. Yoshihito Kano, Jonathan D Cook, Jeﬀrey E Lee, Michael Ohh. New structural and functional insight
into the regulation of Ras. Semin. Cell Dev. Biol. 2016.10; 58; 70-78
28. Hiroko Nagata, Mina Nakagawa, Yuki Nishimura-Sakurai, Yu Asano, Tomoyuki Tsunoda, Masato Miyoshi,
Shun Kaneko, Fumio Goto, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako Murakawa, Sayuri Nitta,
Yasuhiro Itsui, Seishin Azuma, Sei Kakinuma, Naoko Tojo, Shuji Tohda, Yasuhiro Asahina, Mamoru
Watanabe. Serial measurement of Wisteria ﬂoribunda agglutinin positive Mac-2-binding protein is usefulfor predicting liver ﬁbrosis and the development of hepatocellular carcinoma in chronic hepatitis C patientstreated with IFN-based and IFN-free therapy. Hepatol Int. 2016.11; 10(6); 956-964
29. Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Toru Nakata, Tatsuro Murano, Go Ito,
Hiromichi Shimizu, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Akihiro
Araki, Kazuo Ohtsuka, Ryuichi Okamoto, Mamoru Watanabe. PGE2 is a direct and robust mediator ofanion/ﬂuid secretion by human intestinal epithelial cells. Sci Rep. 2016.11; 6; 36795
30. Masanori Kobayashi, Shigeru Oshima, Chiaki Maeyashiki, Yoichi Nibe, Kana Otsubo, Yu Matsuzawa,
Yasuhiro Nemoto, Takashi Nagaishi, Ryuichi Okamoto, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru
Watanabe. The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonicdevelopment. Sci Rep. 2016.11; 6; 36780
31. Toshiyuki Kojima, Kiichiro Tsuchiya, Shinji Ikemizu, Soichiro Yoshikawa, Yoshinori Yamanishi, Mamoru
Watanabe, Hajime Karasuyama. Novel CD200 homologues iSEC1 and iSEC2 are gastrointestinal secretorycell-speciﬁc ligands of inhibitory receptor CD200R. Sci Rep. 2016.11; 6; 36457― 323 ―

Systemic Organ Regulation

Systemic Organ Regulation

32. Hitomi Takada, Kaoru Tsuchiya, Yutaka Yasui, Natsuko Nakakuki, Nobuharu Tamaki, Shoko Suzuki,
Hiroyuki Nakanishi, Jun Itakura, Yuka Takahashi, Masayuki Kurosaki, Yasuhiro Asahina, Nobuyuki
Enomoto, Namiki Izumi. Irregular vascular pattern by contrast-enhanced ultrasonography and high serum
Lens culinaris agglutinin-reactive fraction of alpha-fetoprotein level predict poor outcome after successful
radiofrequency ablation in patients with early-stage hepatocellular carcinoma. Cancer Med. 2016.11;
5(11); 3111-3120
33. Ryuichi Okamoto, Mamoru Watanabe. Functional relevance of intestinal epithelial cells in inﬂammatory
bowel disease. Nihon Rinsho Meneki Gakkai Kaishi. 2016.11; 39(6); 522-527
34. Toshiaki Watanabe, Yoichi Ajioka, Keiichi Mitsuyama, Kenji Watanabe, Hiroyuki Hanai, Hiroshi Nakase,
Reiko Kunisaki, Keiji Matsuda, Ryuichi Iwakiri, Nobuyuki Hida, Shinji Tanaka, Yoshiaki Takeuchi, Kazuo
Ohtsuka, Kazunari Murakami, Kiyonori Kobayashi, Yasushi Iwao, Masakazu Nagahori, Bunei Iizuka,
Keisuke Hata, Masahiro Igarashi, Ichiro Hirata, Shin-Ei Kudo, Takayuki Matsumoto, Fumiaki Ueno, Gen
Watanabe, Masahiro Ikegami, Yoko Ito, Koji Oba, Eisuke Inoue, Naoki Tomotsugu, Toru Takebayashi,
Kenichi Sugihara, Yasuo Suzuki, Mamoru Watanabe, Toshifumi Hibi. Comparison of Targeted vs Random
Biopsies for Surveillance of Ulcerative Colitis-Associated Colorectal Cancer. Gastroenterology. 2016.12;151(6); 1122-1130[Misc]
1. Ryuichi Okamoto, Mamoru Watanabe. Role of epithelial cells in the pathogenesis and treatment ofinﬂammatory bowel disease. J. Gastroenterol. 2016.01; 51(1); 11-21
2. Klionsky DJ, Oshima S, et al. （2387/1536 ）. Guidelines for the use and interpretation of assays formonitoring autophagy (3rd edition). Autophagy. 2016.01; 12(1); 1-222[Conference Activities & Talks]
1. Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyoshi M, Asano Y, Nagata H,
Goto F, Otani S, Kitahata F, Murakawa M , Itsui Y, Nakagawa M, Azuma S, Watanabe M. A Model forInteraction between HBV and Host Cells Derived from Human Induced Pluripotent Stem Cells. The 25th
Asian Paciﬁc Association for the Study of the Liver 2016.02.22 International Convention Center Pamir,Tokyo（JAPAN）
2. Asahina Y. 【HCV 2】Chair . The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22
International Convention Center Pamir, Tokyo（JAPAN）
3. Asahina Y. 【HCV 5】Chair. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22
International Convention Center Pamir, Tokyo（JAPAN）
4. Nakagawa M. 【HCV 】Chair. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22
International Convention Center Pamir, Tokyo（JAPAN）
5. Kawai-Kitahata F, Asahina Y, Tanaka S, Kakinuma S, Murakawa M, Nitta S, Otani S, Goto F, Nagata
H, Kaneko S, Asano Y, Miyoshi M, Tsunoda T, Azuma S, Itsui Y, Nakagawa M, Tanabe M, Maekawa
S, Enomoto N, Watanabe M. Comprehensive analyses of mutations and hepatitis B virus integrationin hepatocellular carcinoma. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22
International Convention Center Pamir, Tokyo（JAPAN）
6. Nagata H, Sakurai Y, Nakagawa M, Kakinuma S, Azuma S, Murakawa M, Nitta S, Itsui Y, Asahina Y,
Watanabe M. Serial changes in M2BPGi levels as predictors of ﬁbrosis and HCC in chronic hepatitis C.
The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22 International Convention Center
Pamir, Tokyo（JAPAN）
7. Nitta S, Asahina Y, Wakita T, Kato T. In vitro characterization and drug sensitivity analysis for HCV
NS5A resistance-associated variants. The 25th Asian Paciﬁc Association for the Study of the Liver2016.02.22 International Convention Center Pamir, Tokyo（JAPAN）
8. Nakagawa M. 【HCV 11】Chair. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.23
International Convention Center Pamir, Tokyo（JAPAN）― 324 ―

Systemic Organ Regulation

Systemic Organ Regulation

9. Nakagawa M. 【HCV 12】Chair. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.23
International Convention Center Pamir, Tokyo（JAPAN）
10. Matsumoto T, Motoya S, Watanabe K, Hisamatsu T, Nakase H, Yoshimura N, Ishida T, Kato S, Nakagawa
T, Esaki M, Nagahori M, Matsui T, Naito Y, Kanai T, Suzuki Y, Nojima M, Watanabe M, Hibi T.
Comparison of adalimumab monotherapy and a combination with azathioprine for patients with Crohns Disease: A prospective, multicentre, open-labeled clinical trial (DIAMOND Study). 11th Congress of
ECCO - European Crohn s and Colitis Organisation 2016.03.18 Amsterdam（Nederland）
11. Matsuoka K, Saito E, Fujii T, Takenaka K, Iwamoto F, Kimura M, Nagahori M, Ohtsuka K, Watanabe M. Eﬃcacy and safety of sequential rescue therapy with inﬂiximab and tacrolimus in patients withcorticosteroid-refractory Ulcerative Colitis. 11th Congress of ECCO - European Crohn s and Colitis
Organisation 2016.03.19 Amsterdam（Nederland）
12. Kobayashi T, Suzuki Y, Motoya S, Hirai F, Ogata H, Ito H, Sato N, Ozaki K, Watanabe M, Hibi T.
Factors associated with the ﬁrst trough level of inﬂiximab at week 2 that predicts short- and long-term
outcomes in Ulcerative Colitis. 11th Congress of ECCO - European Crohn s and Colitis Organisation
2016.03.19 Amsterdam（Nederland）
13. Watanabe M. Gut Microbiota, Epithelial Cells and Stem Cell Therapy in IBD. AGA/CSG IBD Symposium2016.04.08 Baijing（China）
14. Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyoshi M, Asano Y, Nagata H ,Goto F, Otani S, Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Watanabe M. A new model
for studying interaction between hepatitis B virus and host cells derived from human induced pluripotent
stem cells. EASL The International Liver Congress 2016 2016.04.14 Barcelona（Spain）
15. Takenaka K, Ohtsuka K, Kitazume Y, Nagahori M, Matsuoka K, Fujii T, Kimura M, Fujioka T, Watanabe
M. Enteroscopic and MR ﬁndings of small intestine in Crohn s disease. The 7th APTC 2016.04.20 Tokyo（JAPAN）
16. Tetsuya Nakamura. 【Session 2: Gut homeostasis and IBD】Intestinal stem cell transplantation andregenerative medicine. The 102nd JSGE-The 5th International Forum 2016.04.21 Tokyo（JAPAN）
17. Kazuo Ohtsuka, Kento Takenaka, Mamoru Watanabe. 【Session 3: Imaging for follow up of IBD】
Assessment of small intestinal lesions of Crohn s disease. The 102nd JSGE-The 5th International Forum2016.04.21 Tokyo（JAPAN）
18. Ryuichi Okamoto, Mamoru Watanabe. 【Session 4: Novel therapeutic approaches and emerging issues in
IBD】Application of regenerative medicine for the treatment of inﬂammatory bowel disease. The 102nd
JSGE-The 5th International Forum 2016.04.21 Tokyo（JAPAN）
19. Masanori Kobayashi, Shigeru Oshima, Yoishi Nibe, Chiaki Maeyashiki, Mamoru Watanabe. UBA52,
ubiquitin hybrid protein, regulates NF κ B signaling. The 102nd JSGE-The 5th International Forum2016.04.21 Tokyo（JAPAN）
20. Kentaro Takenaka, Kazuo Ohtsuka, Yoshio Kitazume, Masakazu Nagahori, Katsuyoshi Matsuoka, Toshimitsu Fujii, Maiko Kimura, Tomoyuki Fujioka Mamoru Watanabe. Magnetic resonance evaluation for deep
small intestinal strictures in patients with Crohn s disease. The 102nd JSGE-The 5th International
Forum 2016.04.21 Tokyo（JAPAN）
21. Mamoru Watanabe. Closing Remarks. The 102nd JSGE-The 5th International Forum 2016.04.22 Tokyo（JAPAN）
22. Kano Y, Jen Jen Yeh, Zhong-Yin Zhang, Ohh M. Novel treatment strategy for pancreatic cancer bytargeting the undruggable Ras oncoprotein. AACR Special Conference on Pancreatic Cancer 2016.05.14
Orlando（USA)23. Shuji Hibiya, Kiichiro Tsuchiya, Tomoaki Shirasaki, Keita Fukushima, Shigeru Oshima, Ryuichi Okamoto,Tetsuya Nakamura, Mamoru Watanabe. Construction of in vitro model for inﬂammatory bowel diseaseusing primary colonic organoid. DDW2016 2016.05.21 San Diego（USA）― 325 ―

Systemic Organ Regulation

Systemic Organ Regulation

24. Kohei Suzuki, Satoru Fujii, Ami Kawamoto, Fumiaki Ishibashi, Toru Nakata, Go Ito, Hiromichi Shimizu,
Kiichiro Tsuchiya, Tetsuya Nakamura, Kazuo Otsuka, Ryuichi Okamoto, Mamoru Watanabe. Singlecell level analysis reveals hoterogeneous expression of stem-cell speciﬁc genes in human small intestinalorganoids. DDW2016 2016.05.21 San Diego（USA）
25. Takashi Nagaishi, Arisa Tokai, Taro Watabe, Nisha Jose, Akinori Hosoya, Motomi Yamazaki, Richard
S.Blumberg, Mamoru Watanabe. CEACAM1 may resulate BCR signaling in the activated B cells.
DDW2016 2016.05.21 San Diego（USA）
26. Taichi Matsumoto, Wakana Mochizuki, Yoichi Nibe, Shintaro Akiyama, Yuka Matsumoto, Kengo Nozaki,
Tomohiro Mizutani, Shigeru Ochima, Mamotu Watanabe, Tetsuya Nakamura. Rethinol stimulates proliferation of epithelial stem cells of the proximal colon through a retinoic acid-indeoendent mechanism.
DDW2016 2016.05.21 San Diego（USA）
27. Taku Kobayashi, Yasuo Suzuki, Satoshi Motoya, Fumihito Hirai, Haruhiko Ogata, Hiroaki Ito, Noriko
Sato, Kunihiko Ozaki, Mamoru Watanabe, Toshifumi Hibi. Factors Associated With the First TroughLevel of Inﬂiximab at Week 2 That Predicts Short- and Long-Term Outcomes in Ulcerative Colitis.
DDW2016 2016.05.21 San Diego（USA）
28. Emma Calabrese, Torsten Kucharzik, Christian Maaser, Giovanni Maconi, Deike Strobel, Stephanie Wilson, Francesca Zorzi, Kerri L. Novak, David H. Bruining, Marietta Iacucci, Mamoru Watanabe, Elisabetta
Lolli, Carlo Chiaramonte, Stephen B. Hanauer, Remo Panaccione, Francesco Pallone, Subrata Ghosh.
Inter-Observer Agreement in Bowel Ultrasonography for Diagnostic Assessment in Patients With Crohn’s
Disease. DDW2016 2016.05.21 San Diego（USA）
29. Toru Nakata, Go Ito, Hiromichi Shimizu, Satoru Fujii, Kohei Suzuki, Fumiaki Ishibashi, Ami Kawamoto,Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Ryuichi Okamoto, Mamoru Watanabe. Jagged1
is indispensable for the maintenance of tumor stem cell niche in APC-Deﬁcient intestinal adenomas.
DDW2016 2016.05.22 San Diego（USA）
30. Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Toru Nakata, Go Ito, Hiromichi Shimizu,
Tomohiro Mizutani, Kiichiro Tsuchiya, Tetsuya Nakamura, Kazuo Ohtsuka, Ryuichi Okamoto, Mamoru
Watanabe. In Vitro organoid model reveals PGF2 as a Robust Mediator of chloride & water secretion bt
human intestinal epithelial cells. DDW2016 2016.05.23 San Diego（USA）
31. Kazuo Ohtsuka, Kento Takenaka, Toshimitsu Fujii, Katsuyoshi Matsuoka, Masakazu Nagahori, Kengo
Nozaki, Masayoshi Fukuda, Mamoru Watanabe. Mucosal Healing is imoietant for better outcomes ofendosdopic balloon dilatation for smakk intestinal stricture of crohn’s disease. DDW2016 2016.05.23 San
Diego（USA）
32. Chiaki Maeyashiki, Shigeru Oshima, Yoishi Nibe, Masanori Kobayashi, Mamoru Watanabe. Indentiﬁcation of autophagic regulators as therapeutic targets in intestinal epithelial cells. DDW2016 2016.05.23
San Diego（USA）
33. Tetsuya Nakamura. 【Innovative Approaches to Curing Intestinal Failure: The Future of Cell and Tissue
Therapy】Therapeutic Transplantation of Human Intestinal Stem Cells. DDW2016 2016.05.23 San Diego（USA）
34. Katsuyoshi Matsuoka, Makoto Naganuma, Toshiyuki Matsui, Makoto Arai, Satoshi Tanida, Kazuya Kitamura, Noriyuki Horiki, Mikihiro Fujiya, Masaki Iimuro, Keiichi Mitsuyama, Masayuki Saruta, Takayuki
Matsumoto, Hiroko Nebiki, Motohiro Esaki, Fukunori Kinjo, Kiyoshi Oketani, Seiichiro Hojo, Hideto
Akama, Toshio Imai, Hirohito Tsubouchi, Toshifumi Hibi, Takanori Kanai. Safety, Tolerability and Eﬃcacy of E6011, Anti-Human Fractalkine Monoclonal Antibody, in the First-in-Patient Study for Crohn’s
Disease. DDW2016 2016.05.23 San Diego（USA）
35. Kiichiro Tsuchiya, Shuji Hibiya, Keita Fukushima, Tomoaki Shirasaki, Ryuichi Okamoto, Tetsuya Nakamura, Mamoru Watanabe. ATOH1 Protein stabillzation in tumor acquires the morphological change tosignet ring cell enrichment in colon cancer. DDW2016 2016.05.24 San Diego（USA）
36. Masanori Kobayashi, Shigeru Oshima, Yoishi Nibe, Chiaki Maeyashiki, Mamoru Watanabe. UBA52,
ubiquitin hybrid ribisomal protein, regulates NF-κ B signaling in colon cancer. DDW2016 2016.05.24
San Diego（USA）― 326 ―

Systemic Organ Regulation

Systemic Organ Regulation

37. Takayuki Matsumoto, Satoshi Motoya, Kenji Watanabe, Tadakazu Hisamatsu, Hiroshi Nakase, Naoki
Yochimura, Tetsuya Ishida, Shingo Kato, Tomoo Nakagawa, Motohiro Esaki, Masakazu Nagahori, Toshiyuki
Matsui, Yuji Naito, Takanori Kanai, Yasuo Suzuki, Masanori Nojima, Mamoru Watanabe, Toshifumi Hibi.
Comparison of abalimumab monotherapy and a combination with azathioprone for patients with crogn’s
disease:A prospective, multicenter, open-labeled clinical trial (DIAMOND Study). DDW2016 2016.05.24
San Diego（USA）
38. Asahina Y. Hepatocarcinogenesis after hepatitis C viral eradication.. The 10th APASL Single Topic
Conference 2016.06.08 Kaoshiung（Taiwan）
39. Tokai A, Nagaishi T, Watabe T, Jose N, Hosoya A, Yamazaki M, Onizawa M, Richard S. Blumberg,
Watanabe M . Activated B cell receptor signaling may be regulated by CEACAM1. FOCIS 2016 2016.06.23
Boston40. Meng-Tzu Weng, Katsuyoshi Matsuoka, Mamoru Watanabe, JY Lee, Sang Hyoung Park, Suk-Kyun Yang,
Shu-Chen Wei. Thromboembolic events associated with inﬂammatory bowel disease in Asia: incidence
and possible risk factors analysis. AOCC2016 2016.07.07 Kyoto International Conference Center, Kyoto（JAPAN）
41. Shinta Mizuno, Kosaku Nanki, Katsuyoshi Matsuoka, Keiko Ono, Makoto Mutaguchi, Mari Arai, Shinya
Sugimoto, Hiroki Kiyohara, Kozue Takeshita, Makoto Naganuma, Takanori Kanai. Eﬃcacy and safety of
fecal microbiota transplantation on ulcerative colitis. AOCC2016 2016.07.07 Kyoto International Conference Center, Kyoto（JAPAN）
42. Tetsuya Nakamura. Stem-Cell Based Therapies for Intestinal Diseases. AOCC 2016 2016.07.08 Kyoto
International Conference Center, Kyoto（JAPAN）
43. Katsuyoshi Matsuoka, Naoki Yoshimura, Mamoru Watanabe, Satoshi Motoya, Keiichi Tominaga, Ryuichi
Iwakiri, Kenji Watanabe, Toshifumi Hibi, For the AJM300 Study Group. AJM300, an oral α 4 integrin
antagonist, as induction therapy for active ulcerative colitis: a randomized trial. AOCC2016 2016.07.08
Kyoto International Conference Center, Kyoto（JAPAN）
44. Tomoaki Shirasaki, Kiichiro Tsuchiya, Shuji Hibiya, Ryu Nishimura, Keita Fukushima, Sho Watanabe,
Ryuichi Okamoto, Tetsuya Nakamura and Mamoru Watanabe. Stimulation with the ligands of Toll likereceptors in primary human organoids. AOCC2016 2016.07.08 Kyoto International Conference Center,
Kyoto（JAPAN）
45. Ami Kawamoto, Go Ito, Toru Nakata, Satoru Fujii, Kohei Suzuki, Fumiaki Ishibashi, Hiromichi Shimizu,
Kiichiro Tsuchiya, Tetsuya Nakamura, Ryuichi Okamoto, Mamoru Watanabe. OLFM4 and UBD are
up-regulated in the inﬂamed intestinal epithelia of IBD patients by synergy of Notch signaling and TNFα. AOCC2016 2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）
46. Maiko Kimura, Katsuyoshi Matsuoka, Eiko Saito, Toshimitsu Fujii, Kento Takenaka, Masakazu Nagahori, Kazuo Ohtsuka, Mamoru Watanabe. Salvage therapy for corticosteroid-refractory ulcerative colitis
patients. AOCC2016 2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）
47. Kento Takenaka, Kazuo Ohtsuka, Yoshio Kitazume, Masakazu Nagahori, Katsuyoshi Matsuoka, Toshimitsu Fujii, Maiko Kimura, Tomoyuki Fujioka, Mamoru Watanabe. Enteroscopic and MR ﬁndings of small
bowel in Crohn s disease. AOCC2016 2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）
48. Minami Hama, Ayumi Hayashi, Tomohiro Mizutani, Satoru Fujii, Tatsuro Murano, Kiichiro Tsuchiya, Tetsuya Nakamura, Ken Watanabe, Norio Shimizu, Ryuichi Okamoto, Mamoru Watanabe. Prevalence of viral
DNA in the mucosal tissue and cultured organoids of inﬂammatory bowel disease patients. AOCC2016
2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）
49. Toshifumi Hibi, Satoshi Motoya, Kori Wallace, Andreas Lazar, Yasuko Nishimura, Morio Ozawa, Roopal
Thakkar, Anne M. Robinson, Mamoru Watanabe. Adalimumab 80 mg Every Other Week in JapanesePatients with Crohn s Disease Who Lost Response to Maintenance Therapy. AOCC2016 2016.07.08
Kyoto International Conference Center, Kyoto（JAPAN）
50. Shuji Hibiya, Kiichiro Tsuchiya, Tomoaki Shirasaki, Keita Fukushima, Shigeru Oshima, Ryuichi Okamoto,Tetsuya Nakamura, Mamoru Watanabe. Mouse colonic organoid acquires wnt-independent survival property after long-term inﬂammation. AOCC2016 2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）― 327 ―

Systemic Organ Regulation

Systemic Organ Regulation

51. Taro Watabe, Takashi Nagaishi, Arisa Tokai, Akinori Hosoya, Nisha Jose, Yudai Kojima, Motomi Yamazaki, Richard S. Blumberg, Mamoru Watanabe. BCR signaling in the activated B cells may be regulated
by CD66a. AOCC2016 2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）
52. Toshimitsu Fujii, Kento Takenaka, Maiko Kimura, Katsuyoshi Matsuoka, Masakazu Nagahori, Kazuo
Ohtsuka, Mamoru Watanabe. The evaluation for small bowel strictures on MR enterocolonography inpatients with Crohn s disease. AOCC2016 2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）
53. Kiichiro Tsuchiya, Shuji Hibiya, Keita Fukushima, Tomoaki Shirasaki, Shigeru Oshima, Ryuichi Okamoto,Tetsuya Nakamura, Mamoru Watanabe. Atoh1 protein stabilization in colon tumor acquires the phenotype
of signet ring cell carcinoma with cancer stem cell enrichment. AOCC2016 2016.07.08 Kyoto International
Conference Center, Kyoto（JAPAN）
54. Masakazu Nagahori. Anal ﬁstula - Seton ﬁrst or anti-TNF ﬁrst : anti-TNF ﬁrst. AOCC2016 2016.07.09
Kyoto International Conference Center, Kyoto（JAPAN）
55. Kazuo Ohtsuka, Kento Takenaka, Toshimitsu Fujii, Katsuyoshi Matsuoka, Masakazu Nagahori, Maiko
Kimura, Masayoshi Fukuda, Nobuhiro Katsukura, Mamoru Watanabe. Mucosal healing improves prognosis of endoscopic balloon dilatation for small intestinal strictures of Crohn s disease. AOCC2016
2016.07.09 Kyoto International Conference Center, Kyoto（JAPAN）
56. Kiichiro Tsuchiya. 【Poster Oral：Group 4】Chair. AOCC2016 2016.07.09 Kyoto International ConferenceCenter, Kyoto（JAPAN）
57. Nagaishi T, Tokai A, Watabe T, Jose N, Hosoya A, Yamazaki M, Blumberg RS, Watanabe M. BCR
signaling in the activated B cells may be regulated by CEACAM1. Mucosal Immunology Course &Symposium (MICS）2016 2016.07.29 Toronto（Canada）
58. Horita N, Demitrack ES, Giﬀord GB, Keeley TM, Siebel CW, Samuelson LC. Notch1 and Notch2 regulate
epithelial cell proliferation in mouse and human gastric corpus. The 2016 Gordon Research Conferenceon Notch Signaling in Development, Regeneration & Disease 2016.08.01 Lewiston（USA）
59. Watanabe M. Stem Cell Replacement in Gut. FNM2016 2016.08.26 San Francisco（USA）
60. Watanabe M. Intestinal Epithelial Stem Cells for the Treatment of Colitis.. eMed Meeting 2016 2016.10.04
Germany
61. Watanabe M. Gut Microbiota, Epithelial Cells and Stem Cell Therapy in GI disease. Microbiome Science
Days 2016.10.07 Germany
62. Tetsuya Nakamura. Therapeutic transplantation of intestinal stem cells. International Conference on
Bio-fabrication and Bio-monitoring (ICBB 2016) 2016.10.14 National Taiwan University, Taipei（China）
63. Toshimitsu Fujii, Kento Takenaka, Maiko Kimura, Katsuyoshi Matsuoka, Masakazu Nagahori, Kazuo
Ohtsuka, Mamoru Watanabe. MR enterocolonography evaluation for small bowel strictures in patientswith Crohn s disease: comparison with balloon enteroscopy. UEGW2016 2016.10.18 Vienna（Austria）
64. Hibiya S, Tsuchiya K, Watanabe S, Shirasaki T, Oshima S, Okamoto R, Nakamura T, Watanabe M.
Construction of in vitro model of ulcerative colitis using mouse primary colonic organoid. UEGW2016
2016.10.18 Vienna（Austria）
65. Hosoya A, Nagaishi T, Tokai A, Watabe T, Jose N, Kojima Y, Yamazaki M, Richard S. Blumberg,
Watanabe M. CEACAM1 regulation of BCR signaling in the activated B cells. UEGW2016 2016.10.19
VIENNA
66. Asahina Y. Recent progress in anti-HCV therapy and challenges toward HCC eradication.. Academic
Forum of Liver Disease in Northeast China and the Annual Convention for the Study of Liver Diseases in
Jilin Province in 2016. 2016.10.29 Changchun（China）
67. Watanabe M. 【Innovative Topics-L5】Chair. APDW2016 2016.11.03 Kobe convention center, KOBE（JAPAN）
68. Watanabe M. 【Hands-on Seminar：Clinical Science】Chair. APDW2016 2016.11.03 Kobe convention
center, KOBE（JAPAN）― 328 ―

Systemic Organ Regulation

Systemic Organ Regulation

69. Asahina Y. 【International Session（Symposium）1】Genomics of hepatocellular carcinoma: Hepatitis
virus infection and hepatocarcinogenesis. Chair . JDDW2016 2016.11.03 Kobe Cinvention Center（KobeJAPAN）
70. Kiichiro Tsuchiya. 【Digital Poster：Lower GI "Cell/Molecular Biology/Pathology 1"】Chair. APDW2016
2016.11.03 Kobe convention center, KOBE（JAPAN）
71. Tsuchiya K, Hibiya S, Fukushima K, Shirasaki T, Oshima S, Okamoto R, Nakamura T, Watanabe M.
Atoh1 protein stabilization in colon tumor acquires the morphological change to signet ring cell carcinoma
with cancer stem cell enrichment. APDW2016 2016.11.03 Kobe convention center, KOBE（JAPAN）
72. Fujii T, Takenaka K, Kitazume Y, Kimura M, Matsuoka K, Nagahori M, Ohtsuka K, Watanabe M. MR
enterocolonography can detect small bowel strictures and predict surgery in patients with Crohn s disease:
Comparison with balloon enteroscopy. APDW2016 2016.11.03 Kobe convention center, KOBE（JAPAN）
73. Watabe T, Nagaishi T, Suzuki M, Yamazaki M, Onizawa M, Jose N, Tokai A, Hosoya A, Kojima Y,
Watanabe M. Type 2 TNF receptor signaling in colonic epithelia may be involved in the development ofcolitis-associated carcinogenesis. APDW 2016 2016.11.03 Kobe Cinvention Center（Kobe JAPAN）
74. Watabe T, Nagaishi T, Tokai A, Jose N, Hosoya A, Kojima Y, Yamazaki M, Richard S. Blumberg,
Watanabe M. CD66a regulation of BCR signaling in the activated B cells . APDW 2016 2016.11.04 Kobe
Cinvention Center（Kobe JAPAN）
75. Watanabe M.【IBD Consensus Symposium：Asia Paciﬁc Consensus on IBD Management】Chair. APDW2016
2016.11.05 Kobe convention center, KOBE（JAPAN）
76. Takenaka K, Ohtsuka K, Watanabe M. Magnetic resonance evaluation for small bowel strictures of Crohns disease: comparison with balloon enteroscopy. APDW2016 2016.11.05 Kobe convention center, KOBE（JAPAN）
77. Shun Kaneko, Sei Kakinuma, Yasuhiro Asahina, Akihide Kamiya, Masato Miyoshi, Tomoyuki Tsunoda,
Sayuri Nitta, Ayako Sato, Yu Asano, Hiroko Nagata, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako
Murakawa, Yasuhiro Itsui, Mina Nakagawa, Seishin Azuma, Mamoru Watanabe. Genetically modiﬁed
human induced pluripotent stem cell-derived hepatic progenitor-like cell lines as a model for interaction
between hepatitis B virus and host cells. AASLD The Liver meeting 2016 2016.11.11 Boston（USA）
78. Wai-Kay Seto , Yasuhiro Asahina , Cheng-Yuan Peng , Carol Stanciu , Djamal Abdurakhmanov , John F.
Flaherty , Kyungpil Kim , Anuj Gaggar , Mani Subramanian, Fehmi Tabak, Tuan T. Nguyen, Henry LikYuen Chan. Reduced Changes In Bone Mineral Density In CHB Patients Receiving Tenofovir Alfenamide(TAF) Com-pared With Tenofovir disoproxil fumarate. AASLD The Liver meeting 2016 2016.11.11 Boston（USA）
79. Tetsuya Nakamura. Stem cell-based therapies for intestinal diseases. International Conference on Recent
Advances in Inﬂammation 2016 2016.11.11 National Cheng Kung University, Tainan（Taiwan）
80. Watanabe M. Inﬂammation-related Carcinogenesis in Intestine.. 2016 TMDU-TMU Joint Symposium2016.11.12 Taipei Medical University,Taipei（China）
81. Kawamoto A, Nakata T, Fujii S, Suzuki K, Ishibashi F, Shimizu H, Tsuchiya K, Nakamura T, Okamoto
R, Watanabe M. Notch signaling and TNF-α synergistically up-regulate stem cell-speciﬁc genes, OLFM4and UBD, in the inﬂamed intestinal epithelia of IBD patients. 2016 Advances in Inﬂammatory Bowel
Diseases, Crohn s & Colitis Foundation of America’s Clinical & Research Conference (CCFA) 2016.12.10
Orlando, Florida― 329 ―

Systemic Organ Regulation

Systemic Organ Regulation

Specialized Surgeries< Division of Specialized Surgeries>
Professor: Hiroyuki UETAKE
Associate Professor: Yoshinori INOUE
Junior Associate Professor: Toshiaki ISHIKAWA, Tsuyoshi NAKAGAWA, Kentaro OKAMOTO
Assistant Professor: Takahiro TOYOHUKU, Goshi ODA< Department of Professional Development>
Junior Associate Professor: Toshifumi KUDO
< Department of Translational Oncology>
Associate Professor: Megumi ISHIGURO< Graduate School Student>
Kimihiro IGARI, Masato NISHIZAWA, Yohei YAMAMOTO,
Tsuyoshi ICHINOSE, Mio FUKUDA(1)

OutlineDivision of Specialized Surgeries have been launched in April 2015, which consists of 4 clinical departments in
the Medical Hospital:- Division of Chemotherapy and Oncosurgery- Division of Vascular Surgery- Division of Breast Surgery- Division of Pediatric surgery(2)

Research

Main themes of our research activities- Identiﬁcation of prognostic factors and the predictive factors for chemo-responsiveness in gastrointestinal andbreast cancer, by molecular biological technique and immuno-histochemoical approach- Micro circulation in severe ischemic extremity- Relation between vascular disease and periodontitis- Development of new device for evaluating hemodynamics- Development of safety central venous catheterization- Establishment of telemedicine(3)

Education

Main objective in the graduate course is to bring up the well-rounded surgeons who has international andscientiﬁc feelings.

― 330 ―

Systemic Organ Regulation

Systemic Organ Regulation(4)

Publications[Original Articles]
1. Masuda T, Ishikawa T, Mogushi K, Okazaki S, Ishiguro M, Iida S, Mizushima H, Tanaka H, Uetake H,
Sugihara K.. Overexpression of the S100A2 protein as a prognostic marker for patients with stage II and
III colorectal cancer. Int J Oncol. 2016; 48(3); 975-982
2. Ishikawa T, Uetake H, Murotani K, Kobunai T, Ishiguro M, Matsi S, Sugihara K.. Genome-wide DNA
Copy-number Analysis in ACTS-CC Trial of Adjuvant Chemotherapy for Stage III Colonic Cancer. Anticancer Research. 2016; 36; 853-860
3. Baba H, Ishikawa T, Mogushi K, Ishiguro M, Uetake H, Tanaka H, Sugihara K.. Identiﬁcation of SATB1
as a speciﬁc biomarker for lymph node metastasis in colorectal cancer. Anticancer Research. 2016; 36;4069-4076
4. Matsusaka S, Cao S, Hanna DL, Sunakawa Y, Ueno M, Mizunuma N, Zhang W, Yang D, Ning Y, Stintzing
S, Sebio A, Stremitzer S, Yamauchi S, Parekh A, Okazaki S, Berger MD, El-Khoueiry R, Mendez A,
Ichikawa W, Loupakis F, Lenz HJ.. CXCR4 polymorphism predicts progression-free survival in metastatic
colorectal cancer patients treated with ﬁrst-line bevacizumab-based chemotherapy. Pharmacogenomics J.
2016; [ Epub ahead of print]
5. Igari K, Inoue Y, Iwai T.. The epidemiologic and clinical ﬁndings of patients with Buerger disease. Ann
Vasc Surg. 2016; 30; 263-269
6. Zainuer A, Inoue Y, Kudo T, Toyofuku T, Igari K, Kawano T.. Usefulness of the tranfer function index
for diagnosing peripheral arterial disease in patients with arterial calciﬁcation. J Med Dent Sci. 2016;
63(1); 29-35
7. Igari K, Kudo T, Toyofuku T, Inoue Y.. A novel approach to infrainguinal arteries with chronic total
occlusions using the high frequency vibrational device. Int Angiol. 2016; 35(2); 212-216
8. Igari K, Kudo T, Toyofuku T, Inoue Y.. The outcomes of endovascular aneurysm repair with the chimney
technique for juxtarenal aneurysms. Ann Throac Cardiovasc Surg. 2016; 22(3); 174-180
9. Yamamoto Y, Igari K, Toyofuku T, Kudo T, Inoue Y.. Late stent graft infection after the emergency
endovascular repair of a secondary iliac artery-enteric ﬁstula treated with graft removal and in situ aortic
reconstruction using femoral veins. Ann Thorac Cardiovasc Surg. 2016; [ Epub ahead of print]
10. Yamamoto Y, Kudo T, Igari K, Toyofuku T, Inoue Y.. Radial artery aneurysm in the anatomical snuﬀ
box: A case report and literature review. Int J Surg Case Rep. 2016; 27; 44-47
11. Igari K, Kudo T, Toyofuku T, Inoue Y.. Relationship of inﬂammatory biomarkers with severity of peripheral arterial disease. Int J Vasc Med. 2016; 6015701
12. Igari K, Kudo T, Toyofuku T, Inoue Y.. A case of successful coil embolization for a late-onset type Ia
endoleak after endovascular aneurysm repair with the chimney technique. Case Rep Vasc Med. 2016;
5307416
13. Igari K, Kudo T, Toyofuku T, Inoue Y.. Endothelial dysfunction of patients with peripheral arterial
disease measured by peripheral arterial tonometry. Int J Vasc Med. 2016; 3805380
14. Igari K, Kudo T, Toyofuku T, Inoue Y.. The relationship between endothelial dysfunction and endothelial
cell markers in peripheral arterial disease. PLoS ONE. 2016; 11(11); e0166840
15. Igari K, Inoue Y, Iwai T.. An experimental model of peripheral vascular disease involving the travenous
injection of oral bacteria. Ann Vasc Dis. 2016; 9(4); 267-271[Conference Activities & Talks]
1. Okamoto K, Tani Y, Yamaguchi T, Ogino K, Tsuchioka T, Matsudera S, Watanabe S, Kato H.. A Caseof Meckel’s diverticulum torsion, correctly diagnosed by preoperative computed tomography.. 24th Asian
Association Pediatric Surgeons 2016.05.24 Fukuoka (Japan)― 331 ―

Systemic Organ Regulation

Systemic Organ Regulation

2. Igari K, Kudo T, Toyofuku T, Inoue Y.. Relationship of inﬂammatory biomarkers, PTX3 with severityof peripheral arterial disease.. 84th European Atherosclerosis Society Congress 2016.05.31 Innsbruck(Austria)
3. Kajiwara Y, Ishiguro M, Teramukai S, Matsuda C, Fujii S, Kinugasa Y, Nakamoto Y, Kotake M, Sakamoto
Y, Kurachi K, Maeda A, Komori K, Tomita N, Simada Y, Takahashi K, Kotake K, Watanabe M, Mochizuki
H, Sugihara K.. A randomized phase III trial of 1-year adjuvant chemotherapy with oral tegafur-uracil(UFT) vs. surgery alone in stage II colon cancer.. American Society of Clinical Oncology (ASCO) 2016
Annual Meeting 2016.06.04 Chicago (USA)
4. Miura T, Sunami E, Kusumoto T, Ota M, Yoshida K, Tomita N, Maeda A, Mochizuki I, Okabe M,
Kunieda K, Yamauchi J, Itabashi M, Kotake K, Takahashi K, Baba H, Boku N, Aiba K, Ishiguro M,
Morita S, Sugihara K.. Planned safety analysis of the ACTS-CC 02 trial: A randomized phase III trialof S-1/oxaliplatin (SOX) versus UFT/LV as adjuvant chemotherapy for high-risk stage III colon cancer..
American Society of Clinical Oncology (ASCO) 2016 Annual Meeting 2016.06.04 Chicago (USA)
5. Igari K, Kudo T, Toyofuku T, Inoue Y.. Total endovascular treatment for acute deep venous thrombosis
by catheter-directed thrombolysis.. Asian Society for Vascular Surgery 2016 2016.10.21 Singapore
6. Ishiguro M.. Colorectal cancer database in Japan..
2016.11.05 Seoul (Korea)

68th Congress of the Korean Surgical Society.

7. Yamamoto Y, Ichinose T, Nakamura M, Nishizawa M, Igari K, Toyofuku T, Kudo T, Inoue Y.. Multiple
recurrent pseudoaneurysms after EVAR in a patient with Behçet s disease.. The 13th Tokyo Shanghai
International Symposium for Vascular Surgery 2016.11.13 Tokyo (Japan)― 332 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

Cardiovascular Medicine

Professor
Mitsuaki Isobe
Clinical Professor
Kenzo Hirao
Endowed Professor
Takashi Ashikaga
Associate Professor
Tetsuo Sasano (Graduate School of Health Care Sciences,
Biofunctional Informatics)
Masahiko Goya Kiyoshi Nobori (Clinical Research Center )Junior Associate Professor
Yasuhiro Maejima, Yusuke Ebana (Medical Research InstituteBio-informational Pharmacology)
Assistant Professor
Mihoko Kawabata, Ryoko Azuma, Shunji Yoshikawa Takeshi Sasaki,
Yuji Konishi, Takanobu Yamamoto, Yu Hatano
Specially-Appointed Professor
Shingo Maeda, Kentaro Takahashi
Endowed Assistant Professor
Daisuke Ueshima
Joint Research Assistant Professor
Yasuhiro Shirai
Graduate Student
Yusuke Ito, Natsuko Tamura, Ryo Watabe, Tetsuo Ohmi, Tomoko Manno,
Kei Takayama, Ryota Iwatsuka, Yoichi Otaki, Atsuhiko Yagishita,
Tatsuya Fujinami, Tomoyo Sugiyama, Osamu Inaba, Masahito Suzuki,
Naoyuki Miwa, Rena Nakamura, Tomoyuki Umemoto, Norifumi Murai, Riri Watanabe,
Masahiro Yamazoe, Shunsuke Hirose, Tetsuo Yamaguchi, Hironori Sato, Maki Ohno,
Keiichi Hishikari, Takashi Nakagawa, Hidetsugu Nomoto, Yasuaki Tanaka,
Tetsumin Lee, Yukihiro Inamura, Mie Ochida, Tomomasa Takamiya Kensuke Hirasawa,
Ohnish Kentaro, Mituhisa Asano, Nobutaka Kato, Naohiko Kawaguchi,
Shinsuke Iwai, Yuki Osaka, Nobuyuki Kagiyama, Shunsuke Kuroda, Takuro Nisimura,
Junji Matuda, Makoto Araki, Kusa Shigeki, Kazuya Yamao, Takamitu Takagi,
Yoshihisa Kanechi, Nagamine Shyo, Rei Masuda, Hidehiro Shirai, Naoko Kato,
Takashi Nida

(1)

Research

The purposes of our investigation are to reveal the etiology and pathophysiology of cardiovascular diseases,
thereby developing novel technologies for diagnosis and treatment. For that purpose we investigate clinical casesand experimental animal models. Our faculty members pursue a wide variety of basic research, ranging frominvestigations on the molecular mechanism of heart failure to the development of novel interventional devices
for patients with angina pectoris. Current strengths of the program include innovation of the next-generation
drug-eluting balloon for percutaneous coronary intervention by the Chief of this Department, Mitsuaki Isobe― 333 ―

Systemic Organ
Organ Regulation
Regulation
Systemic

MD, and molecular cardiology under Yasuhiro Maejima MD, PhD. We also actively investigate immunocardiology, the molecular mechanism of pulmonary hypertension and periodontitis-associated cardiovascular diseases.
1) Clinical study of gene therapy for coronary artery disease (Isobe)2) Clinical study for treatment of acute coronary syndrome (Isobe, Ashikaga, Yoshikawa)
3) Molecular mechanism and treatment of myocardial ischemia and reperfusion injury (Isobe)4) Molecular mechanism and treatment of coronary restenosis and vascular disease (Isobe)5) Gene therapy of myocarditis and cardiac chronic rejection (Isobe, Suzuki)6) Cardiac rejection and immunological tolerance (development of safe immunosuppressive therapy) (Isobe,
Suzuki)7) Treatment of heart failure and cardiomyopahty by myocardial regeneration (Isobe, Maejima)
8) Regulation of arteriosclerosis by targeting transcription factors (Isobe, Maejima)
9) Gene therapy of vascular disease (Isobe)10) Diagnostic imaging of aortitis (Isobe)11) Molecular mechanism and treatment of aortitis (Isobe, Maejima)
12) Assessment of vascular endothelial dysfunction in vasculitis, heart failure and arrhythmia (Isobe)13) Application in gene therapy for heart failure and cardiomyopathy (Isobe, Maejima)14) Molecular system of myocardial remodeling in heart failure and ventricular hypertrophy (Isobe)
15) Therapy of sleep apnea syndrome with heart failure (Isobe)16) Assessment by imaging of coronary artery and cardiac function (Isobe, Tezuka)17) System of origin with tachyarrhythmias (particularly supraventricular tachycardia) (Hirao)18) Medical therapy and ablation for tachyarrhythmias (Hirao)
19) Research for the conduction of atrio-ventricular node (Hirao)
20) Research and Therapy for arrhythmia by using cardioendoscopy (Hirao)21) Research of atrial ﬁbrillation from origin of pulmonary vein (Hirao)
22) Research of genetic factor with atrial ﬁbrillation (Hirao)
23) Research of ablation for atrial ﬁbrillation (Hirao, Goya)
There are many fruitful collaborative eﬀorts between our department and other departments with the TMDU,
such as the Department of Bio-informational Pharmacology, Medical Research Institute. Collaborations withother institutions are also common. Especially, we closely collaborate with the Department of Advanced Clinical
Science and Therapeutics, University of Tokyo (Junichi Suzuki MD, PhD). Our cardiologists continuously contribute to establish evidence-based cardiovascular medicine through clinical researches. So far, we are engagedin over ten clinical studies. The targets of our clinical research include heart failure, ischemic heart disease,arrhythmia, cardiac imaging and Takayasu arteritis.

(2)

Education

The Department of Cardiovascular Medicine at Tokyo Medical and Dental University (TMDU) primarily aims
at oﬀering patient-centered care for every person who suﬀer from cardiovascular diseases, including ischemic
heart diseases, arrhythmia, heart failure, valvular disorders and vasculitis. Our cardiologists are experts inelectrophysiology, interventional cardiology, heart failure, and cardiac imaging who make full use of state-of-theart diagnostic tests and therapeutic procedures to provide high-quality care for every patient. We also actively
engage in basic and clinical research to elucidate the mechanism of heart & vessel disorders for providing novel
therapeutic strategies to the patients of cardiovascular diseases. In addition to high-quality patient care andinnovative research, our faculty members are vigorously involved in the education and training to the young
physicians and researchers specializing in cardiovascular medicine. Thus, we are continuously making every
eﬀort to serve the highest quality of patient care, education and innovative research of cardiovascular medicine.
(3)

Clinical Services & Other Works

Our clinical training program provides the trainee with outstanding skills in clinical cardiology. The trainees
will develop their clinical knowledge, clinical judgment, procedural skills and interpersonal skills required as a
specialist in cardiovascular diseases. The program provides clinical cardiology training not only at the University
Hospital but also at our outstanding aﬃliate hospitals (N=22), including Kameda General Hospital, Musashino
Red-Cross Hospital, Tsuchiura Kyodo Hospital and Yokosuka Kyosai Hospital. Our training program for re-― 334 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

search emphasizes developing academic cardiologists who will become leaders in cardiovascular research. The
program oﬀers training of basic, clinical and translational researches not only at the Graduate School of ourdepartment but also at other departments with the TMDU and at other institutions described above.
(4)

Publications[Original Articles]
1. Yuki Hasegawa, Satomi Hamada, Takuro Nishimura, Takeshi Sasaki, Yusuke Ebana, Mihoko Kawabata,
Masahiko Goya, Mitsuaki Isobe, Takatoshi Koyama, Tetsushi Furukawa, Kenzo Hirao, Tetsuo Sasano.
Novel Dielectric Coagulometer Identiﬁes Hypercoagulability in Patients with a High CHADS2 Score without Atrial Fibrillation. PLoS ONE. 2016; 11(6); e0156557
2. Kentaro Takahashi, Tetsuo Sasano, Koji Sugiyama, Junko Kurokawa, Noriko Tamura, Yurie Soejima, Motoji Sawabe, Mitsuaki Isobe, Tetsushi Furukawa. High-fat diet increases vulnerability to atrial arrhythmia
by conduction disturbance via miR-27b. J. Mol. Cell. Cardiol.. 2016.01; 90; 38-46
3. Hirofumi Zempo, Jun-Ichi Suzuki, Masahito Ogawa, Ryo Watanabe, Mitsuaki Isobe. A diﬀerent role ofangiotensin II type 1a receptor in the development and hypertrophy of plantaris muscle in mice. J. Appl.
Genet.. 2016.02; 57(1); 91-97
4. Shiheido Y, Maejima Y, Suzuki J, Aoyama N, Kaneko M, Watanabe R, Sakamaki Y, Wakayama K,
Ikeda Y, Akazawa H, Ichinose S, Komuro I, Izumi Y, Isobe M. Porphyromonas gingivalis, a periodontal
pathogen, enhances myocardial vulnerability, thereby promoting post-infarct cardiac rupture. J. Mol.
Cell. Cardiol.. 2016.04;
5. Yoichi Otaki, Yusuke Ebana, Shunji Yoshikawa, Mitsuaki Isobe. Dielectric permittivity change detects
the process of blood coagulation: Comparative study of dielectric coagulometry with rotational thromboelastometry. Thromb. Res.. 2016.06; 145; 3-11
6. Hirokazu Ohigashi, Natsuko Tamura, Yusuke Ebana, Masayoshi Harigai, Yasuhiro Maejima, Takashi
Ashikaga, Mitsuaki Isobe. Eﬀects of immunosuppressive and biological agents on refractory Takayasu
arteritis patients unresponsive to glucocorticoid treatment. J Cardiol. 2016.08;
7. Lian Liu, Yusuke Ebana, Jun-Ichi Nitta, Yoshihide Takahashi, Shinsuke Miyazaki, Toshihiro Tanaka,
Masatoshi Komura, Mitsuaki Isobe, Tetsushi Furukawa. Genetic Variants Associated With Susceptibility
to Atrial Fibrillation in a Japanese Population. Can J Cardiol. 2016.11;
8. Hirofumi Zempo, Jun-Ichi Suzuki, Masahito Ogawa, Ryo Watanabe, Katsuhito Fujiu, Ichiro Manabe,
Simon J Conway, Yoshiaki Taniyama, Ryuichi Morishita, Yasunobu Hirata, Mitsuaki Isobe, Ryozo Nagai.
Inﬂuence of periostin-positive cell-speciﬁc Klf5 deletion on aortic thickening in DOCA-salt hypertensive
mice. Hypertens. Res.. 2016.11; 39(11); 764-768[Misc]
1. Yasuhiro Maejima, Mitsuaki Isobe, Junichi Sadoshima. Regulation of autophagy by Beclin 1 in the heart.
J. Mol. Cell. Cardiol.. 2016.06; 95; 19-25
2. Yusuke Ebana. Ethics in Clinical Research Obstet. Gynecol.. 2016.10; 83(10); 1119-1123[Conference Activities & Talks]
1. Yusuke Ebana Hiroko Kohbata, Sayako Takahashi, Masayuki Yoshida. Genetic counselling for a case withbreast cancer who underwent a whole-exome sequencing in a private company. The 13th International
Congress Society of Human Genetics 2016.04.06
2. Kiyoshi Nobori. HOW TO BALANCE THE NEED FOR GLOBAL TRIALS AND THE NEED FOR REGIONAL DATA? : Dosing and PK matters. 13th Global CardioVascular Clinical Trials Forum 2016.12.01
Embassy of France, 4101 Reservoir Rd NW, Washington D.C. 20007, USA― 335 ―

Systemic Organ Regulation

Systemic Organ Regulation

Anesthesiology

Professor: Koshi Makita
Associate Professor: Tokujiro Uchida
Junior Associate Professor:Jiro Kurata, Seiji Ishikawa,
Satoshi Toyama
Assistant Professor: Maiko Satomoto, Akio Masuda, Mamoru Yamamoto
Takashi Hakusui, Yusuke Ito, Akihiro Haramo, Tetsuo Koyanagi,
Akiko Kitajo, Izumi Ebana
Staﬀ: Sonomi Tanaka,Sayomi Nagai, Takeshi Kasuga, Manami Tanaka
Yohei Tetsuyuki, Atsushi Ito
Resident: Tsubasa Akune, Tomoe Tajiri
Postgraduate Student:Fukami Nakajima,Yutaka Miura,
Yuzuru Inatomi,Hiroyuki Ito, Hiroyuki Kobinata, Eri Ikeda,
Tomoko Ishibashi, Hiroto Yamamoto, Yudai Yamamoto, Yu Qi,
Zhang Shuo, ZhongLiang Sun
Research Student:Tianjao Li, Suet May Chan
(1)

OutlineA comprehensive understanding of research trends, research methods, and analysis of results by introducing thelatest papers published in prestigious journals related to anesthesiology.
(2)

Research

1)Discovering most eﬀective ventilation methods for injury lungs.
2) Therapeutic mechanism of mesenchymal stem cell for lung injury (rat and mouse model)
3) Studies on the central nervous system eﬀects of general anesthetics by human electrocorticogram and functional neuroimaging.
4) Studies on the mechanisms of cerebral pain processing and pain chroniﬁcation by human functional magnetic
resonance imaging and positron emission tomography.
5) Studies on the eﬀects of protective one-lung ventilation on ventilatory mechanics.
6) Epidemiologic studies to identify incidence of, and risk factors for postoperative acute kidney injury in patients undergoing liver resection.
7) Studies on the eﬀect of anesthetics on the developing brain.

(3)

Publications[Original Articles]
1. Mizue Hobo, Akihito Uezato, Mitsunori Nishiyama, Mayumi Suzuki, Jiro Kurata, Koshi Makita, Naoki
Yamamoto, Toru Nishikawa. A case of malignant catatonia with idiopathic pulmonary arterial hypertension treated by electroconvulsive therapy. BMC Psychiatry. 2016; 16(1); 130
2. Emi Nakamura, Hiroyuki Kinoshita, Guo-Gang Feng, Hisaki Hayashi, Maiko Satomoto, Motohiko Sato,
Yoshihiro Fujiwara. Sevoﬂurane Inhalation Accelerates the Long-Term Memory Consolidation via Small― 336 ―

Systemic Organ Regulation

Systemic Organ Regulation

GTPase Overexpression in the Hippocampus of Mice in Adolescence. PLoS ONE. 2016; 11(9); e0163151
3. Maiko Satomoto, Zhongliang Sun, Yushi U Adachi, Koshi Makita. Sugammadex-Enhanced Neuronal
Apoptosis following Neonatal Sevoﬂurane Exposure in Mice. Anesthesiol Res Pract. 2016; 2016; 9682703
4. Yukako Obata, Yushi U Adachi, Katsumi Suzuki, Taiga Itagaki, Hiromi Kato, Maiko Satomoto, Yoshiki
Nakajima. The Inﬂuence of Diﬀerences in Solvents and Concentration on the Eﬃcacy of Propofol at
Induction of Anesthesia. Anesthesiol Res Pract. 2016.01; 2016; 9178523
5. Z Sun, M Satomoto, Y U Adachi, H Kinoshita, K Makita. Inhibiting NADPH oxidase protects against
long-term memory impairment induced by neonatal sevoﬂurane exposure in mice. Br J Anaesth. 2016.05;
117(1); 80-86
6. Kazawa M, Fukagawa A, Ito H, Toyama S, Makita K. Risk of diﬃcult intubation may increase with age
in pediatric patients with pycnodysostosis. Pediatric Anesthesia. 2016.05; 5; 567-568
7. Maiko Satomoto, Zhongliang Sun, Yushi U Adachi, Koshi Makita. Neonatal Sevoﬂurane Exposure Induces
Adulthood Fear-induced Learning Disability and Decreases Glutamatergic Neurons in the Basolateral
Amygdala. J Neurosurg Anesthesiol. 2016.11;
8. Yu Qi, Tokujiro Uchida, Mamoru Yamamoto, Yudai Yamamoto, Koji Kido, Hiroyuki Ito, Nagara Ohno,
Miho Asahara, Yoshitsugu Yamada, Osamu Yamaguchi, Chieko Mitaka, Makoto Tomita, Koshi Makita.
Perioperative Elevation in Cell-Free DNA Levels in Patients Undergoing Cardiac Surgery: Possible Contribution of Neutrophil Extracellular Traps to Perioperative Renal Dysfunction. Anesthesiology Researchand Practice. 2016.11; 2794364[Books etc]
1. Lohser J, Ishikawa S. Chapter 6, Clinical management of one-lung ventilation. Slinger P Ed., Principles
and practice of anesthesia for thoracic surgery. Springer, 2011, pp 83-101..
2. Lohser J, Ishikawa S. Chapter 5, Physiology of the lateral decubitus position, open chest, and one-lungventilation. Slinger P Ed., Principles and practice of anesthesia for thoracic surgery. Springer, 2011, pp
71-82..
[Misc]
1. Koichi Nakazawa, Ken Shinoda. An overview of incidence and outcome of perioperative pulmmonary
aspiration The Japanese Journal of Anesthesiology . 2016.01; 65(1); 4-12
2. Jiro Kurata. Pulse oximeter: Oxygenation and hemodynamics at a glance Tha Japanese Journal of Acute
Medicine. 2016.01; 40(1); 5-8
3. Maiko Satomoto, Koshi Makita. Anesthesia-induced neurotoxicity in an animal model of the developing
brain: mechanism and therapies. Neural Regen Res. 2016.09; 11(9); 1407-1408[Conference Activities & Talks]
1. Induction and mechanism of cytotoxicity by long-term propofol treatment in human induced pluripotent
stem cell-derived neurons. 2016.05.26
2. A retrospective survey on Combined Spinal Epidural Anesthesia for colectomy with cardio-pulmonary
complications during past eleven years. 2016.09.03
3. Koji Kido, Hiroyuki Ito, Yudai Yamamoto, Tokujiro Uchida, Koshi Makita. Dose-dependent Cytotoxicityof Propofol in Cultured Human-induced Pluripotent Stem Cell-derived Neurons. Anesthesiology 2016
annual meeting 2016.10.23 Chicago
4. SUN Zhongliang, Maiko Satomoto, RI Shinnbinn, Koshi Makita. Sugammadex-enhanced neuronal apoptosis following neonatal sevoﬂurane exposure in mice. The 36th Annual Meeting lf the Japan Society for
Clinical Anesthesia 2016.11.03 Kochi― 337 ―

Systemic Organ Regulation

Systemic Organ Regulation

Cardiovascular Surgery

Professor Hirokuni ARAI
Associate Professor Tomohiro MIZUNO
Junior Associate Professor Keiji OI
Assistant Professor Masafumi YASHIMA, Tsuyoshi HACHIMARU, Eiki NAGAOKA, Tatsuki FUJIWARA,
Masashi TAKESHITA
Graduate Student Hidehito KUROKI, Tatsuki FUJIWARA, Dai TASAKI
Hospital Staﬀ 2(1)

Research

1) Developing safe and high quality surgical strategy in coronary artery bypass grafting surgery.
2) Developing new surgical technique for ischemic heart disease3) Developing new surgical technique for beating mitral valve surgery
4) Clinical research for artiﬁcial heart5) Research for new regenerative therapy for failing heart to recover cardiac function(2)

Education

Cardiovascular Surgery is a branch of surgery which deals with heart and vascular (mainly aortic) disease.
Main objective of our department in the graduate course is to provide medical students an opportunity to study
surgical anatomy, pathophysiology, pharmacology, and advanced surgical treatment for heart and aortic disease.
Students are also taught basic research for the surgical treatment for heart and aortic disease. We also provide
clinical training program for young surgeon to obtain Japanese cardiovascular surgical board.
(3)

Clinical Performances

Our department provides well-advanced surgical treatment of heart and aortic surgery. We perform oﬀ-pumpcoronary artery bypass grafting for more than 90% of patients with coronary artery disease, mitral valve repair,
not valve replacement, for almost all patients with mitral valve regargitation. New surgical reconstruction
technique is provided for patients with functional mitral regurgitation due to severe heart failure. For elderly
patients, we oﬀer minimally invasive aortic surgery such as thoracic endovascular aortic repair (TEVAR) andhybrid aortic surgery without cardiopulmonary bypass for aortic arch and thoracoabdominal aortic disease.
(4)

Publications[Original Articles]
1. Fujiwara T, Sakota D, Ouchi K, Endo S, Tahara T, Murashige T, Kosaka R, Oi K, Mizuno T, Maruyama
O, Arai H.. Optical dynamic analysis of thrombus inside a centrifugal blood pump during extracorporeal
mechanical circulatory support in a porcine model Artif Organs. 2016; In Press;― 338 ―

Systemic Organ Regulation

Systemic Organ Regulation

2. Daisuke Sakota, Tomotaka Murashige, Ryo Kosaka, Tatsuki Fujiwara, Katsuhiro Ouchi, Masahiro Nishida,
Osamu Maruyama. Noninvasive optical imaging of thrombus formation in mechanical circulatory support
devices Journal of Biorheology. 2016; 30; 6-12
3. Shioiri A, Kurumaji A, Takeuchi T, Nemoto K, Arai H, Nishikawa T. . A Decrease in the Volume of Gray
Matter as a Risk Factor for Postoperative Delirium Revealed by an Atlas-based Method. The American
Journal of Geriatric Psychiatry. 2016.07; 24(7); 528-536
4. Minoru Ono, Yoshiki Sawa, Takeshi Nakatani, Ryuji Tominaga, Yoshiro Matsui, Kenji Yamazaki, Yoshikatsu
Saiki, Hiroshi Niinami, Goro Matsumiya, Hirokuni Arai, . Japanese Multicenter Outcomes With the HeartMate II Left Ventricular Assist Device in Patients With Small Body Surface Area. Circ. J.. 2016.08;
80(9); 1931-1936
5. Daisuke Sakota, Tatsuki Fujiwara, Katsuhiro Ouchi, Katsuyuki Kuwana, Hiroyuki Yamazaki, Osamu
Maruyama. Development of an Optical Detector of Thrombus Formation on the Pivot Bearing of a
Rotary Blood Pump. Artiﬁcial Organs. 2016.09; 40(9); 834-841
6. Tomomi Hasegawa, Munetaka Masuda, Meinoshin Okumura, Hirokuni Arai, Junjiro Kobayashi, Yoshikatsu
Saiki, Kazuo Tanemoto, Hiroshi Nishida, Noboru Motomura. Trends and outcomes in neonatal cardiac
surgery for congenital heart disease in Japan from 1996 to 2010. Eur J Cardiothorac Surg. 2016.10; 1-7
7. Tomohiro Mizuno, Koso Egi, Kenji Sakai, Keiji Oi, Tsuyoshi Hachimaru, Tohru Makita, Kiyotoshi Oishi,
Hirokuni Arai. Minimally Circulatory-Assisted On-Pump Beating Coronary Artery Bypass Grafting for
Patients With Complex Conditions for Oﬀ-Pump Surgery. Artif Organs. 2016.10;
8. Munetaka Masuda, Hiroyuki Kuwano, Meinoshin Okumura, Hirokuni Arai, Shunsuke Endo, Yuichiro
Doki, Junjiro Kobayashi, Noboru Motomura, Hiroshi Nishida, Yoshikatsu Saiki, Fumihiro Tanaka, Kazuo
Tanemoto, Yasushi Toh, Hiroyasu Yokomise. Erratum to: Thoracic and cardiovascular surgery in Japan
during 2013 : Annual report by The Japanese Association for Thoracic Surgery. Gen Thorac Cardiovasc
Surg. 2016.12; 64; 496-500[Books etc]
1. Mizuno T, Arai H.. Oﬀ-Pump Coronary Artery Bypass, Chapter 9: Exposure / Chapter 11: Securing theDistal Anastomosis Site: Coronary Artery Occlusion, Shunt, and Blower. Springer Japan, 2016
[Conference Activities & Talks]
1. Tasaki D., Mizuno T., Oi K., Hachimaru T., Nagaoka E., Kuroki H., Fujiwara T., Kinoshita R., Arai
H.. Therapeutic Strategy of Malignant Cardiac Tumor. the 24th Annual meeting of Asian Society for
Cardiovascular ant Thoracic Surgery (ASCVTS2016) 2016.04.07 Taipei,Taiwan
2. Eiki Nagaoka, Tomohiro Mizuno, Keiji Oi, Tuyoshi Hachimaru, Hidehito Kuroki, Dai Tasaki, Tatsuki
Fujiwara, Ryouji Kinoshita, Hirokuni Arai. The Eﬀect of Papillary Muscle Relocation on Posterior LeaﬂetTethering in Functional Mitral Regurgitation. the 24th Annual meeting of Asian Society for Cardiovascular
ant Thoracic Surgery (ASCVTS2016) 2016.04.08 Taipei,Taiwan
3. Keji Oi, Tomohiro Mizuno, Tsuyoshi Hachimaru, Eiki Nagaoka, Hidehito Kuroki, Dai Tasaki, Tatsuki
Fujiwara, Ryoji Kinoshita, Hirokuni Arai. LONG-TERM OUTCOME OF TOTAL ARTERIAL GRAFTCABG WITH SINGLE INTERNAL MAMMARY ARTERY AND OTHER ARTERIAL GRAFTS. the24th Annual meeting of Asian Society for Cardiovascular ant Thoracic Surgery (ASCVTS2016) 2016.04.08
Taipei,Taiwan
4. Kinoshita R., Mizuno T., Hachimaru T., Arai H., Oi K., Nagaoka E., Kuroki H., Tasaki D., Fujiwara T.,
Yashima M., Takeshita M. . Multiple Inﬂammatory Aortic Aneurysms due to Vasculitis. AATS Aortic
Symposium 2016 2016.05.12 New York,USA
5. Mizuno T., Hachimaru T., Oi K., Nagaoka E., Kuroki H., Tasaki D., Fujiwara T., Kinoshita R., Arai
H.. Total Debranching TEVAR for Extended Thoracic Aortic Disease Including Aortic Arch. American
Association for Thoracic Surgery 2016.05.12 New York, USA― 339 ―

Systemic Organ Regulation

Systemic Organ Regulation

6. K Ohuchi, T Mizuno, K Oi, M Yashima, T Hachimaru, E Nagaoka, T Fujiwara, H Kuroki, D Tasaki, M
Takeshita, R Kinoshita, S Endo, M Kanai, S Suzuki, T Kimura, H Arai. Attempt for the Developmentof Cardiac Surgery Training Program Combined with Live Animal Surgery. The 63rd Annual Meeting ofJapanese Association for Animal Science 2016.05.18
7. Mizuno T., Hachimaru T., Oi K., Yashima M., Nagaoka E., Kuroki H., Tasaki D., Fujiwara T., Takeshita
M., Kinoshita R., Arai H.. Surgical Strategy to Balance less Invasiveness with Complete Aortic Repair
and Less Neurologic and Aorta-Related Complications for Extended Thoracic Aortic Disease Including
Aortic Arch. ISMICS(International Society for Minimally Invasive Cardiothoracic Surgery) 2016 Annual
Scientiﬁc Meeting 2016.06.15 Montreal Quebec, Canada
8. Impact of Papillary Muscle Relocation on Prognosis of Patient with Functional Mitral Regurgitation.
2016.07.14 Fukuoka/Japan
9. Fujiwara T, Arai H, Mizuno T, Oi K, Yashima M, Hachimaru T, Nagaoka E, Kuroki H, Tasaki D,
Takeshita M, Kinoshita R, Ohuchi K. Which is a better acute-phase circulatory support for cardiogenic
shock patients in INTERMACS proﬁle-1: a pulsatile VAD or a centrifugal pump?. 24th Annual Congressof the International Society for Rotary Blood Pumps 2016.09.21 Ibaraki
10. Ohuchi K, Mizuno T, Oi K, Yashima M, Hachimaru T, Nagaoka E, Fujiwara T, Kuroki H, Tasaki D,
Takeshita M, Kinoshita R, Endo S, Kanai M, Kimura T, Arai H. Development of Heart Surgery Training
Program Combined with Live Animal Surgery. 24th Congress of the International Society for Rotary
Blood Pumps 2016.09.21
11. Fujiwara T, Arai H, Mizuno T, Oi K, Yashima M, Hachimaru T, Nagaoka E, Kuroki H, Tasaki D,
Takeshita M, Kinoshita R, Ohuchi K. Which is a better acute-phase circulatory support for cardiogenic
shock patients in INTERMACS Proﬁle-1: A pulsatile VAD or a centrifugal pump?. 24th Congress of theInternational Society for Rotary Blood Pumps 2016.09.21
12. Sakota D, Fujiwara T, Ouchi K, Kuwana K, Yamazaki H, Arai H, Maruyama O. Development of an opticalthrombus detector on the pivot bearing of a rotary blood pump. 24th Congress of the International Society
for Rotary Blood Pumps 2016.09.22
13. Arai H.. (Panelist) Coronary Artery Bypass Graft: Minimally Invasive and Hybrid Revascularisation..
30th European Association for Cardio-Thoracic Surgery 2016.10.04 Barcelona, Spain
14. Hirokuni Arai. （Moderator) Coronary Revascularization. ISMICS 2016 Winter Workshop 2016.10.28
Kyoto
15. Mizuno T.. Total debranching TEVAR. ISMICS 2016 Winter Workshop 2016.10.28 Kyoto
16. E Nagaoka, T Mizuno, K Oi, T Hachimaru, H Kuroki, D Tasaki, T Fujiwara, R Kinoshita, H Arai.
On-pump Beating Heart Mitral Valve Repair without Aortic Cross-clamping: Tips and Tricks for Safe
Practice. ISMICS 2016 Winter Workshop 2016.10.29 Kyoto
17. Arai H. Epi-aortic Ultrasound: Standard of Care. AATS International Coronary Congress 2016 2016.11.11
New Delhi, India
18. Arai H. (moderator) Parallel Session V: BITA Grafting: How to Harvest and Use
International Coronary Congress 2016 2016.11.11 New Delhi, India

BITA Grafts. AATS

19. Arai H. (moderator) Parallel Session X: Mini Debates, Hot Topics 2.. AATS International Coronary
Congress 2016 2016.11.12 New Delhi, India
20. Arai H. (moderator) Parallel Session XVI: Management of Ischemic MR, Heart Failure and Unbypassable
Targets. AATS International Coronary Congress 2016 2016.11.13 New Delhi, India
21. Keji Oi, Tomohiro Mizuno, Masafumi Yashima, Tsuyoshi Hachimaru, Eiki Nagaoka, Hidehito Kuroki,
Dai Tasaki, Tatsuki Fujiwara, Masashi Takeshita, Ryoji Kinoshita, Hirokuni Arai. Elevated Preoperative
Hemoglobin A1c Level and Adverse Longterm Outcomes After Coronary Artery Bypass Surgery in Patients
With Renal Dysfunction and Diabetes. American Heart Association 2016 2016.11.14 NewOrleans,USA
22. Arai H. Posterior leaﬂet New Repair Technique. Medtronic Mitral Academy Japan 2016 2016.11.19 Tokyo― 340 ―

Systemic Organ Regulation

Systemic Organ Regulation

23. Arai H. Papillary Muscle Relocation in Functional MR cases. . Medtronic Mitral Academy Japan 20162016.11.20 Tokyo
24. Arai H. Innovation in Cardiac Surgery. the 30th Kalyanakit Kitiyakara Lecture 2016.11.28 Bangkok,
Thailand
25. Arai H. How to improve the quality of modern CABG. Inter-hospital Conference 39 2016.12.17 Bangkok,
Thailand
26. Arai H. Target vessels exposure, ﬂow measurement and ultrasound imaging. 5th RAMA OPCAB Meeting2016.12.19 Bangkok, Thailand
27. Arai H. My approach to reduce Neurological complication in CABG. 5th RAMA OPCAB Meeting2016.12.20 Bangkok, Thailand[Patents]
1. OSTEOSYNTHESIS MEMBER, Announcement Number：CN106137358A― 341 ―

Systemic Organ Regulation

Systemic Organ Regulation

Nephrology

Professor:
Shinichi UCHIDA
Tatemitsu RAI (Dept. of Nephrology and Regional Medicine (Ibaraki))
Associate Professor:
Tomokazu OKADO (Dept. of Blood Puriﬁcation)
Eisei SOHARA
Junior Associate Professor:
Shotaro NAITO
Assistant Professor:
Naohiro NOMURA
Soichiro IIMORI (Dept. of Blood Puriﬁcation)
Hidehiko SATO (Project Assistant Professor)
Daiei TAKAHASHI (Dept. of Blood Puriﬁcation)
Moko ZENIYA (Dept. of Nephrology and Regional Medicine (Ibaraki))
Graduate Student:
Fumiaki ANDO, Yuki YOSHIZAKI, Yohei ARAI, Yuri KASAGIEmi SASAKI, Shintaro MANDAI, Sayaka YOSHIDA
Wang Yuan Long, Hiroaki KIKUCHI, Wakana SHODA,
Hiroko HASHIMOTO
Takuya Fujimaru, Taisuke FURUSHO, Yoshiaki MATSUURA
Hospital Staﬀ:
Norikazu SHOJI (Dept. of Blood Puriﬁcation),
Naohiro TAKAHASHI, Eriko TAKEHARA,
Yuko MATSUMOTO (Dept. of Blood Puriﬁcation),Takashi MIYAZAKI, Emi SAKAMOTO,
Isao KONDO, Soichiro SUZUKI
Technician:Chieko IIJIMA, Motoko CHIGA
Secretary:
Asa MURANO, Yukiko ITO(1)Outline

The policy of the Department of Nephrology is to accomplish trustworthy medicine and to educate excellent
academic scientists and nephrologists. Our department is one of the initial institutes that started the hemodialysis therapy in Japan, and thus, has a long experience of clinical practice of kidney diseases.
We are now investigating pathophysiological mechanisms of various kidney diseases including genetic renal diseases. Furthermore, we are taking a proactive stance in developing innovative therapy. We hope new young
scientists and physicians join us for future science and nephrology.

― 342 ―

Systemic Organ Regulation

Systemic Organ Regulation(2)

Research

The theme of our study is "to investigate the mechanisms of maintaining blood pressure and body ﬂuids
homeostasis regulated by the kidney and to clear the pathophysiology caused by their failure, and to develop
novel strategies for their treatment. This would lead to the development of kidney disease therapy itself andwould also lead to studying for multiple organ failure caused by chronic kidney disease (CKD).
In 2016, our 17 presentations, including 2 oral presentations, were adopted in the annual meeting of American
society of nephrology (ASN KIDNEY WEEK). Moreover, the articles written by Fumiaki Ando and Wakana
Shoda, were accepted by Nature Communication (IF: 11.33) and Kidney International (IF: 7.68) respectivily.
Both of them were presented in "press release".
In addition to them, a lot of our members have got prizes in various medical meetings regardless of whether
they are in or out of the country. Further, comprehensive diagnosis of inherited kidney diseases using next
generation sequencer (NGS) and clinical studies on genome information are now on track and is contributing tovarious genetic diagnosis of many patients. CKD-ROUTE study, which is the clinical cohort study with 1,000
subjects we take the initiative have been ﬁnished the observation period of 3 years, and useful knowledge would
be expected to be obtained.

(3)

Education

Undergraduate education"(Systematic lecture)
For third grade medical students, we are conducting lectures organized in a three-week block form together
with urology and pathology sections. Under the name of "Body Fluid Regulation and Urology" Block, thestudents can learn intensively about kidney and urologic diseases during a period of three weeks. In thislecture, we incorporate PBL (problem-based learning) lectures and lectures held by actual patients, so that thestudents will be able to study independently and bi-directionally.
(Project semester)
We accept several students every year in the project semester, in which they are expected to participate in theforefront research with the assistance of graduate students.
(Clinical clerkship)
For ﬁfth grade students who ﬁnished the systematic lectures and project semester, we provide the pre-clinicalclerkship (PCC) lectures for ten weeks, which are more practical and comprehensive than that the previous
lectures held in the classroom. After PCC, the students will undergo clinical clerkship (CC), in which they will
actually take charge of patients in the hospital ward, and study about kidney diseases while developing theirclinical skills. They will be in charge of one new inpatient each week, make a presentation about their patientat the medical conference every week, and are expected to learn about the pathophysiology of various kidneydiseases in depth.
"Postgraduate education"
After the two-year initial training after graduation, postgraduate doctors will be engaged in clinical training as
nephrologists either in the University Hospital or aﬃliated hospitals as senior trainees, and during this period,
we teach them so that they can be aware about unsolved clinical problems. We are planning to bring them up
as academic doctors .
Research activities in the graduate school are quite active, and by carrying out the state-of-the-art research as
described above, we are training doctors to be able to excel in both basic and clinical works.

(4)

Clinical Services & Other Works

Our department is one of the initial institutes that started the hemodialysis therapy in Japan, and thus, has along experience of clinical practice of kidney diseases. We have close coordination with aﬃliated hospitals, andare performing CKD-ROUTE clinical cohort study stated above in cooperation with 15 of hospitals and we arecoping with revealing the pathophysiology of CKD patients. We have been actively adopting the educational
admission for CKD patients and it has been showing the signiﬁcant inhibitory eﬀect on progression of kidney― 343 ―

Systemic Organ Regulation

Systemic Organ Regulationdisease. For end-stage renal disease patients, vascular access surgery, peritoneal dialysis-related surgery, andinduction of dialysis are consistently carried out in our department.
Our hemopuriﬁcation center is one of the biggest institutions among 42 hospitals belonging to national
universities. In 2015, the number of newly started dialysis patients was in 1st pace, the number of plasma
exchange was 1st place, the number of hemodialysis was 2nd place, and total number of hemopuriﬁcation therapy
was within 2nd place. Furthermore, we developed diagnostic panel of hereditary diseases like nephrogenic
diabetes insipidus, peudohypoaldosteronism type II, and Liddle syndrome, and then we receive requests ofgenetic tests from all over the country.

(5)

Publications[Original Articles]
1. Takeshi Kusuda, Tadashi Hosoya, Takayasu Mori, Katsuhito Ihara, Hidenori Nishida, Motoko Chiga, Eisei
Sohara, Tatemitsu Rai, Ryuji Koike, Shinichi Uchida, Hitoshi Kohsaka. Acquired Gitelman Syndrome inan Anti-SSA Antibody-positive Patient with a SLC12A3 Heterozygous Mutation. Intern. Med.. 2016;
55(21); 3201-3204
2. Wakaba Yamaguchi, Naofumi Yui, Toshikage Nagao, Gaku Oshikawa, Mariko Negi, Soichiro Iimori,Tomokazu Okado, Tatemitsu Rai, Shinichi Uchida. Bence-Jones Protein λ-type Multiple Myeloma
Patient Withdrawn from Maintenance Hemodialysis after Long-term Bortezomib and Dexamethasone
Therapy. Intern. Med.. 2016; 55(3); 263-268
3. Hiroaki Kikuchi, Eiichiro Kanda, Shintaro Mandai, Masanobu Akazawa, Soichiro Iimori, Katsuyuki Oi,
Shotaro Naito, Yumi Noda, Takayuki Toda, Teiichi Tamura, Sei Sasaki, Eisei Sohara, Tomokazu Okado,
Tatemitsu Rai, Shinichi Uchida. Combination of low body mass index and serum albumin level is associated with chronic kidney disease progression: the chronic kidney disease-research of outcomes in treatment
and epidemiology (CKD-ROUTE) study. Clin. Exp. Nephrol.. 2016.02;
4. Shintaro Mandai, Eiichiro Kanda, Soichiro Iimori, Shotaro Naito, Yumi Noda, Hiroaki Kikuchi, Masanobu
Akazawa, Katsuyuki Oi, Takayuki Toda, Eisei Sohara, Tomokazu Okado, Sei Sasaki, Tatemitsu Rai,
Shinichi Uchida. Association of serum chloride level with mortality and cardiovascular events in chronic
kidney disease: the CKD-ROUTE study. Clin. Exp. Nephrol.. 2016.04;
5. Rahman Asadur, Kittikulsuth Wararat, Fujisawa Yoshihide, Suﬁun Abu, Raﬁq Kazi, Hitomi Hirofumi,
Nakano Daisuke, Sohara Eisei, Uchida Shinichi, Nishiyama Akira. Eﬀects of diuretics on sodium-dependent
glucose cotransporter 2 inhibitor-induced changes in blood pressure in obese rats suﬀering from themetabolic syndrome. J Hypertens. 2016.05; 34(5); 893-906
6. Arai Yohei, Kanda Eiichiro, Iimori Soichiro, Naito Shotaro, Noda Yumi, Kawasaki Tomoki, Sato Hidehiko,Ando Ryoichi, Sasaki Sei, Sohara Eisei, Okado Tomokazu, Rai Tatemitsu, Uchida Shinichi. The use ofvitamin D analogs is independently associated with the favorable renal prognosis in chronic kidney diseasestages 4-5: the CKD-ROUTE study. Clin Exp Nephrol. 2016.06;
7. Imai Eri, Kaneko Shuzo, Mori Takayasu, Okado Tomokazu, Uchida Shinichi, Tsukamoto Yusuke. A novel
heterozygous mutation in the ATP6V0A4 gene encoding the V-ATPase a4 subunit in an adult patientwith incomplete distal renal tubular acidosis. Clin Kidney J. 2016.06; 9(3); 424-428
8. Ohkubo Atsushi, Okado Tomokazu, Miyamoto Satoko, Goto Keigo, Yamamoto Motoki, Maeda Takuma,
Itagaki Ayako, Seshima Hiroshi, Kurashima Naoki, Sohara Eisei, Uchida Shinichi, Rai Tatemitsu. Removal Characteristics of Immunoadsorption With the Immusorba TR-350 Column Using Conventionaland Selective Plasma Separators. Ther Apher Dial. 2016.08; 20(4); 360-367
9. Miyamoto Satoko, Ohkubo Atsushi, Seshima Hiroshi, Maeda Takuma, Itagaki Ayako, Kurashima Naoki,
Iimori Soichiro, Naito Shotaro, Sohara Eisei, Rai Tatemitsu, Uchida Shinichi, Okado Tomokazu. Removal Dynamics of Immunoglobulin and Fibrinogen by Conventional Plasma Exchange, Selective Plasma
Exchange, and a Combination of the Two. Ther Apher Dial. 2016.08; 20(4); 342-347
10. Mitani Marie, Furuichi Munehiro, Narumi Satoshi, Hasegawa Tomonobu, Chiga Motoko, Uchida Shinichi,
Sato Seiji. A patient with pseudohypoaldosteronism type II complicated by congenital hypopituitarism
carrying a KLHL3 mutation. Clin Pediatr Endocrinol. 2016.10; 25(4); 127-134― 344 ―

Systemic Organ Regulation

Systemic Organ Regulation

11. Cheung W Pui, Nomura Naohiro, Nair V Anil, Pathomthongtaweechai Nutthapoom, Ueberdiek Lars, Lu
Hua A Jenny, Brown Dennis, Bouley Richard . EGF Receptor Inhibition by Erlotinib Increases Aquaporin
2-Mediated Renal Water Reabsorption. J. Am. Soc. Nephrol.. 2016.10; 27(10); 3105-3116
12. Wakana Shoda, Naohiro Nomura, Fumiaki Ando, Yutaro Mor, Takayasu Mori, Eisei Sohara, Tatemitsu
Rai, Shinichi Uchida. Calcineurin inhibitors block sodium-chloride cotransporter dephosphorylation in
response to high potassium intake Kidney International . 2016.10; 91; 402-411
13. Ando Fumiaki, Sohara Eisei, Morimoto Tetsuji, Yui Naofumi, Nomura Naohiro, Kikuchi Eriko, Takahashi Daiei, Mori Takayasu, Vandewalle Alain, Rai Tatemitsu, Sasaki Sei, Kondo Yoshiaki, Uchida
Shinichi. Wnt5a induces renal AQP2 expression by activating calcineurin signalling pathway. Nat Commun. 2016.11; 7; 13636
14. Imai Eri, Kaneko Shuzo, Mori Takayasu, Okado Tomokazu, Uchida Shinichi, Tsukamoto Yusuke. Erratum:
A novel heterozygous mutation in the ATP6V0A4 gene encoding the V-ATPase a4 subunit in an adultpatient with incomplete distal renal tubular acidosis. Clin Kidney J. 2016.12; 9(6); 871[Misc]
1. Yuki Yoshizaki, Shinichi Uchida. Renal tubular acidosis(Fanconi syndrome included) JMEDJ. 2016.04;[Conference Activities & Talks]
1. Sohara E. WNK signaling and KLHL3/Cullin3 ubiquitin ligase complex in salt-sensitive hypertension.
The 89th Annual Meeting of the Japanese Pharmacological Society 2016.02.09
2. Arai Yohei, Sohara Eisei, Takahashi Daiei, Oda Mayumi, Ko Shigeru, Ko Minoru, Rai Tatemitsu, Uchida
Shinichi. CaMk1g phosphorylates WNK4 S1190. The 59th Annual Meeting of the Japanese Society ofNephrology 2016.06.18 Yokohama
3. Wakana Shoda, Naohiro Nomura, Fumiaki Ando, Eisei Sohara, Tatemitsu Rai, Shinichi Uchida. Highpotassium intake promotes urinary potassium excretion by activation of calcineurin and dephosphorylationof Na-Cl cotransporter(NCC). The 59th Annual Meeting of the Japanese Society of Nephrology 2016.06.18
Yokohama
4. Emi Sasaki, Eisei Sohara, Koichiro Susa, Takayasu Mori, Kiyoshi Isobe, Yuya Araki, Yuichi Inoue,
Tatemitsu Rai, Shinichi Uchida,. Generation and analysis of KLHL3 knockout mice. The 59th Annual
meeting of Japanese Society of Nephrology 2016.06.18 Yokohama
5. Yuanlong Wnang, Eisei Sohara, Moko Zeniya, Yutaro Mori, Daiei Takahashi, Shotaro Naito, Tatemitsu
Rai, Shinichi Uchida. High Potassium Regulates Vascular Tonus through KLHL2 Protein and WNK3SPAK-NKCC1 Phosphorylation Cascade. The 59th Annual Meeting of the Japanese Society of Nephrology
2016.06.18
6. Taisuke Furusho, Noriyuki Toshima, Takashi Miyazaki, Itaru Yasuda, Shotaro Takakuwa, Makiko Konishi,
Minami Toda, Yatsuka Saito, Suguru Hirasawa, Noriyoshi Toki, Takaie Kuki, Hiromi Kihira, Manabu
Haneda, Yasuhide Nishio. Relationship between arterial and interstitial lesions in IgA nephropathy. The
59th Annual Meeting of the Japanese Society of Nephrology 2016.06.19 Yokohama
7. Yuki Yoshizaki, Eisei Sohara, Takayasu Mori, Daiei Takahashi, Moko Zeniya, Yutaro Mori, Yuya Araki,
Fumiaki Ando, Tatemitsu Rai, Shinichi Uchida. Drug Repositioning Screening for Keap1-Nrf2 Binding
Inhibitors by Using Fluorescent Correlation Spectroscopy. The 59th Annual Meeting of the JapaneseSociety of Nephrology 2016.06.19 Yokohama
8. Ando F, Sohara E, Yui N, Nomura N, Kikuchi E, Takahashi D, Mori T, Rai T, Sasaki S, and Uchida S..
Calcineurin is a target for the treatment of nephrogenic diabetes insipidus. . 3rd Next Generation Kidney
Research Meeting. 2016.08 Tokyo
9. Soichiro Suzuki, et al.. Two cases of Hypercalcemia leading to End Stage Kidney Disease. The 46th
Eastern Regional Meeting of the Japanese Society of Nephrology 2016.10.08
10. Wakana Shoda, Naohiro Nomura, Fumiaki Ando, Yutaro Mori, Takayasu Mori, Eisei Sohara, Tatemitsu
Rai, Shinichi Uchida. Calcineurin rapidly dephosphorylates sodium-chloride cotransporter in response tohigh potassium intake. The 26th Ochanomizu Jin Kenkyukai 2016.10.27 Tokyo― 345 ―

Systemic Organ Regulation

Systemic Organ Regulation

11. Eriko Takehara, Daiei Takahashi, Yujiro Nakano, Koichiro Susa, Moko Zeniya, Hidehiko Sato, Soichiro
Iimori, Naohiro Nomura, Shotaro Naito, Eisei Sohara, Tomokazu Okado, Tatemitsu Rai, Shinichi Uchida.
Severe alkarosis and hypokaremia in a patient with pancreatic neuroendocrine tumor. Rinsho Taieki
2016.10.29
12. Zeniya M, Mori T, Yui N, Nomura N, Chiga M, Sohara E, Rai T, Uchida S.. The proteasome inhibitor
bortezomib attenuates renal ﬁbrosis in mice.. The 49th Annual Meeting of American Society of Nephrology
2016.11 Chicago
13. Hiroaki Kikuchi, Naohiro Nomura, Yoji Andrew Minamishima, Tatemitsu Rai, Shinichi Uchida andEisei Sohara.. Targeted Metabolomic Analysis of Kidney from the Subtotal Nephrectomy Mouse Modelof Chronic Kidney Disease. The 49th Annual Meeting of American Society of Nephrology. 2016.11.15
Chicago.
14. Yutaro Mori, Shintaro Mandai, Takayasu Mori, Eisei Sohara, Tatematsu Rai, Shinichi Uchida. Generationof p62/SQSTM1 (p62) KO Cell Lines Using CRISPR/Cas9 -The Role of p62 on WNK1 Regulation by
Changes of Extracellular Potassium Concentration-. The 49th Annual Meeting of the American Societyof Nephrology 2016.11.17 Chicago
15. Emi Sasaki, Koichiro Susa, Takayasu Mori, Kiyoshi Isobe, Yuya Araki, Yuichi Inoue, Tatemitsu Rai,
Shinichi Uchida, Eisei Sohara. Generation and Analysis of KLHL3 Knockout Mice. The 49th Annual
Meeting of American Society of nephrology 2016.11.18 Chicago
16. Naofumi Yui, Sei Sasaki, Shinichi Uchida. Regulated Ser-261 Dephosphorylation Combined with Ser-256
and Ser-269 Phosphorulation in the C-terminus of Aquaporin-2 Water Channel. American Society ofNephrology 2016.11.18 Chicago
17. Yohei Arai, Eiichiro Kanda, Soichiro Iimori, Shotaro Naito, Yumi Noda, Sei Sasaki, Eisei Sohara, Tomokazu
Okado, Tatemitsu Rai, Shinichi Uchida. Low White Blood Cell Count Is Independently Associated with
the Progression of Chronic Kidney Disease in the Elderly: The CKD-ROUTE Study. The 49th Annual
Meeting of American Society of Nephrology 2016.11.18 Chicago
18. Wakana Shoda, Naohiro Nomura, Fumiaki Ando, Yutaro Mori, Takayasu Mori, Eisei Sohara, Tatemitsu
Rai, Shinichi Uchida. Calcineurin Rapidly Dephosphorylates Sodium-ChlorideCotransporter in Response
to High Potassium Intake. The 49th Annual Meeting of American Society of nephrology. 2016.11.18
Chicago19. Yuki Yoshizaki, Eisei Sohara, Takayasu Mori, Eriko Kikuchi, Daiei Takahashi, Moko Zeniya, Yuya Araki,
Yutaro Mori, Fumiaki Ando, Tatemitsu Rai, Shinichi Uchida.. Drug Repositioning Screening for Keap1Nrf2 Binding Inhibitors by Using Fluorescent Correlation Spectroscopy. The 49th Annual Meeting ofAmerican Society of Nephrology 2016.11.18 Chicago
20. Takuya Fujimaru, Takayasu Mori, Shintaro Mandai, Motoko Chiga, Hiroaki Kikuchi, Fumiaki Ando,
Yutaro Mori, Naohiro Nomura, Shotaro Naito, Tomokazu Okado, Tatemitsu Rai, Shinichi Uchida, Eisei
Sohara. Development of a Customized Diagnostic Panel for Targeted Exome Sequencing of Polycystic
Kidney Diseases. Kidney Week 2016 2016.11.19 Chicago, IL
21. Daiei Takahashi, Takayasu Mori, Eisei Sohara, Motoko Chiga, Yuichi Inoue, Naohiro Nomura, Moko
Zeniya, Tatemitsu Rai, and Shinichi Uchida. WNK4 regulates adipocyte diﬀerentiation in 3T3-L1 cells.
The 47th Annual Meeting of American Society of Nephrology 2016.11.19 Chicago, IL.
22. Yuya Araki, Tatemitsu Rai, Eisei Sohara, Takayasu Mori, Yuichi Inoue, Yutaro Mori, Shinichi Uchida. The
Pseudohypoaldosteronism Type II-Causing Mutant Cul3 Protein Forms Dimer with KLHL3 and Inhibits
the Degradation of WNK4 with a Dominant-Negative Eﬀect. The 49th Annual Meeting of American
Society of Nephrology. 2016.11.19 Chicago― 346 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

Comprehensive Reproductive Medicine

Professor : Toshiro KUBOTA , Naoyuki MIYASAKA
Associate Professor : Satoshi OBAYASHI
Project Professor : Naoyuki MIYASAKA, Masakazu TERAUCHI
Junior Associate Professor : Naoyuki YOSHIKI, Tatsuya HARADA
Project Associate Professor : Masakazu TERAUCHI
Project Junior Associate Professor: Akira WAKABAYASHI
Assistant Professor : Kimio WAKANA, Tomonori ISHIKAWA,Yuki IWAHARA,Noriko OSHIMA- SUDO,
Shiro HIRAMITSU,Mayumi YOKOTA,Atsushi YAMAMOTO,Takafumi TSUKADA,
Nobuyu KIKIDERA, Asuka HIROSE
Project Assistant Professor : Makiko EGAWA , Ayako FUDONO
HospitalStaﬀ :Maki TAKAO,Yasunori YOSHINO,Atsusi FUSEGI,Reiko NAKAMURA, Miho NAITO,
Takuyuki KUBO
Graduate Student :Reiko SHIRAI, Makoto IIZUKA, Kiyotaka TAKAGI, Izumi HONDA,Asuka HIROSE,
Takashi NAKASUJI,Kazuki SAITO, Akiko FURUSAWA, Takuto MATSUURA, Rie OI,
Takayuki TATSUMI,Masaki SEKIGUCHI,Tamami ODAI,Kenta TAKAHASHI,Mayumi KOBAYASHI,
Ayumi YAMAGUCHI,Yuri SUKENOBE,Nozomi UTIDA(1)

ResearchResearch divisions :1) Research in physiology, endocrinology and metabolism in the reproductive medicine
2) Research of female physical and mental change with aging
3) Pathophysiological examination of gynecological malignant tumor4) Clinical research and basic research in perinatal medicine
Available scientiﬁc procedures :1, Cell culture technique of ovarian granulosa cells, endometrial cells, malignant cells, osteoblast and so on.
2, Determination of intracellular calcium (by Fura 2 method and patch clump )3, Measurement of intra-cellular IP34, Hormonal assay in plasma, urine, follicular ﬂuid (RIA & EIA)
5, Immunohistochemistry with ABC method
6, Analysis of micro-structure with electrical microscopy
7, Determination with molecular biological technique.
8, Physiological determination with isometric tension change
9, Determination of cerebral blood ﬂow with MRI in cerebral infarction
10, Analysis of protein expression with ﬂow cytometry― 347 ―

Systemic Organ
Organ Regulation
Regulation
Systemic(2)

Education

CRM (OB/GY) department has an obligation to oﬀer medical services, education, research as one of the clinicaldepartments in national graduate school, and has duty on making a mutual cooperation with local gynecologicalinstitutions.
Our main objectives are
1, Investigation for a new progress in treatment technique
2, Acquisition of medical knowledge and procedure
3, Providing systemic lecture about women s physiological and pathological change during adolescence throughsenescence.
Aims of research works are focusing on reproductive medicine, perinatal medicine and oncology.
Educational intention in medical doctor course and nursing course includes systemic lectures, clinical conferences
and special lecture by many extramural speakers. During Bed-Side Learning period, students should be treatedas one of medical stuﬀs, attend all of deliveries and be present at gynecological procedure. Several OB/GY
institutions will be provided as an extramural drills.

(3)

Clinical Performances

For intractable sterilization, satisfactory results are obtained with endoscopic examinations and IVF-ET methods. Health care unit for menopausal women was established , where inspections for atherosclerosis, osteoporosis(DEXA), autonomic nervous system are performed, and postmenopausal managements are provided including
HRT, mental care and counseling.
After construction of LDR(labor, delivery, recovery) unit, cure for complicated pregnancies is now carried out,
and cases of deliveries are rising now.
Malignant gynecological tumor is also an important aim of this department, for which surgery, chemotherapy
and radiotherapy with complete cure are applied to patients. For benign tumor and endometriosis, laparoscopic
operations are aggressively performed, whose number is now increasing.

(4)

Publications[Original Articles]
1. Asuka Hirose, Masakazu Terauchi, Mihoko Akiyoshi, Yoko Owa, Kiyoko Kato, Toshiro Kubota. Depressive symptoms are associated with oxidative stress in middle-aged women: a cross-sectional study.
Biopsychosoc Med. 2016; 10; 12
2. Tatsumi T, S.C. Jwa, Kuwahara A, Irahara M, Kubota T, Saito H. No increased risk of major congenital
anomalies or adverse pregnancy or neonatal outcomes following letrozole use in assisted reproductive
technology Human Reproduction. 2016;
3. Yamada I, Hikishima K, Miyasaka N, Kato K, Kojima K, Kawano T, Ito E, Kobayashi D, Eishi Y, Okano
H. Gastric carcinoma: evaluation with diﬀusion-tensor MR imaging and tractography ex vivo. Magn
Reson Imaging. 2016.02; 34(2); 144-151
4. Asuka Hirose, Masakazu Terauchi, Mihoko Akiyoshi, Yoko Owa, Kiyoko Kato, Toshiro Kubota. Low-dose
isoﬂavone aglycone alleviates psychological symptoms of menopause in Japanese women: a randomized,
double-blind, placebo-controlled study. Arch. Gynecol. Obstet.. 2016.03; 293(3); 609-615
5. Saito K, Matsuzaki T, Iwasa T, Miyado M, Saito H, Kubota T, Irahara M, Ogata T, Fukami M.. Blood
allopregnanolone levels in women with polycystic ovary syndrome. Clinical Endocrinology. 2016.04;
6. Saito K, Matsuzaki T, Iwasa T, Miyado M, Saito H, Hasegawa T, Homma K, Inoue E, Miyashiro Y,
Kubota T, Irahara M, Ogata T, Fukami M. Steroidogenic pathways involved in androgen biosynthesis ineumenorrheic women and patients with polycystic ovary syndrome Journal of Steroid Biochemistry andMolecular Biology. 2016.04; 158; 31-37
7. Kon M, Saito K, Mitsui T, Miyado M, Igarashi M, Moriya K, Nonomura K, Shinohara N, Ogata T, Fukami
M.. Copy-number Variations of the Azoospermia Factor Region or SRY Are Not Associated with the Rickof Hypospadias. Sexual Development. 2016.04;― 348 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

8. Haruka Maneyama, Naoyuki Miyasaka, Kimio Wakana, Megumi Nakamura, Yoshio Kitazume, Toshiro
Kubota. Vanishing intravenous leiomyomatosis after hysterectomy: Assessment of the need to perform
complete resection. J. Obstet. Gynaecol. Res.. 2016.04;
9. Takahashi K, Yoshida H. Watanabe R, Ishikawa M, Ikeda SI, Kato T. Metastasis of extra-ammpullary
duodenal adenocarcinoma to the uterine cervix Malaysian Journal of Pathology Malays J Pathol. 2016.04;
38 (1); 45-48
10. Makiko Egawa, Naoyuki Miyasaka, Takuyuki Kubo, Miho Naitou, Takashi Ito, Toshiro Kubota. A caseof puerperal group a streptococcal sepsis complicated by ovarian vein thrombosis. J Obstet Gynaecol.
2016.05; 1-5
11. Ikeda Y, Furusawa A, Kitagawa R, Tokinaga A, Ito F, Ukita M, Nomura H, Yamagami W, Tanabe
H, Mikami M, Takeshima N, Yaegashi N. Practice patterns of adjuvant therapy for intermediate/highrecurrence risk cervical cancer patients in Japan J Gynecol Oncol. 2016.05; 27(3);12. Yoshiki N, Kubota T. Two-port laparoscopic ovarian cystectomy using 3-mm instruments. Gynecol Minim
Invasive Ther. 2016.05; 5(2); 78-80
13. Yoshiki N. Single-incision laparoscopic myomectomy: A review of the literature and available evidence
Gynecol Minim Invasive Ther. 2016.05; 5(2); 54-63
14. Makoto Iizuka, Naoyuki Miyasaka, Yuki Hirose, Mikayo Toba, Shuichi Sakamoto, Toshiro Kubota. Is
there a diﬀerential impact of parity on factors regulating maternal peripheral resistance? Hypertens.
Res.. 2016.06;
15. A Hirose, M Terauchi, M Akiyoshi, Y Owa, K Kato, T Kubota. Subjective insomnia is associated withlow sleep eﬃciency and fatigue in middle-aged women. Climacteric. 2016.08; 19(4); 369-374
16. Yamada I, Hikishima K, Miyasaka N, Kato K, Ito E, Kojima K, Kawano T, Kobayashi D, Eishi Y, Okano
H. q-Space MR imaging of gastric carcinoma ex vivo: correlation with histopathologic ﬁndings. Magn
Reson Med. 2016.08; 76(2); 602-612
17. Dolmans MM and Iwahara Y, Donnez J, Soares M, Vaerman JL , Amorim CA, Poirel H. Evaluation ofminimal disseminated disease in cryopreserved ovarian tissue from bone and soft tissue sarcoma patients
Human Reproduction. 2016.09; 31; 2292-2302
18. Kihara A, Wakana K, Kubota T, Kitagawa M. SLUG expression is an indicator of tumour recurrence inhigh-grade endometrial carcinomas Histopathology. 2016.09; 69(3); 374-382
19. Miyasaka N, Egawa M, Isobe M, Inoue Y, Kubota T. Obstetrical management of patients with extraanatomic vascular bypass grafts due to takayasu arteritis Obstet Gynaecol Res. 2016.10; 42(12); 18641869
20. Yoshii SR, Kuma A, Akashi T, Hara T, Yamamoto A, Kurikawa Y, Itakura E, Tsukamoto S, Shitara
H, Eishi Y, Mizushima N. Systemic Analysis of Atg5-Null Mice Rescued from Neonatal Lethality by
Transgenic ATG5Expression in Neurons Dev Cell. . 2016.10; 10;39(1); 116-130
21. Matsuura T, Otsuka I. Eﬀectiveness of Carboplatin plus Taxane Chemotherapy for Advanced or Recurrent
Uterine Serous Carcinoma Gan To Kagaku Ryoho. 2016.11; 43(11); 1401-1404
22. Yuji Komaki, Keigo Hikishima, Shinsuke Shibata, Tsunehiko Konomi, Fumiko Seki, Masayuki Yamada,Naoyuki Miyasaka, Kanehiro Fujiyoshi, Hirotaka J Okano, Masaya Nakamura, Hideyuki Okano. Functional brain mapping using speciﬁc sensory-circuit stimulation and a theoretical graph network analysis
in mice with neuropathic allodynia. Sci Rep. 2016.11; 6; 3782
23. Miyasaka N, Miura O, Kawaguchi T, Arima N, Morishita E, Usuki K, Morita Y, Nishiwaki K, Ninomiya
H,Gotoh A, Imashuku S, Urabe A, Shichishima T, Nishimura J, Kanakura Y. . Pregnancy outcomes ofpatients with paroxysmal nocturnal hemoglobinuria treated with eculizumab: A Japanese experience andupdated review. Int. j. hematol. .
24. Sekiguchi M, Mioyazaki O, Wada S, Nosaka S, Sago H. Prenatal diagnosis of Pfeiﬀer syndrome type II
using ultralow dose CT {Online}― 349 ―

Systemic Organ
Organ Regulation
Regulation
Systemic[Books etc]
1. Miyasaka N,Miura O. Pregnancy in Paroxysmal Nocturnal Hemoglobinuria. Paroxysmal Nocturnal Hemoglobinuria, 2016
[Misc]
1. Masakazu Terauchi. Menopausal symptoms and the Kampo medicine: Tokishakuyakusan, Kamishoyosan,
and Keishibukuryogan Recent Progress in Kampo Medicine in Obstetrics and Gynecology. 2016.04; 33;
23-30[Conference Activities & Talks]
1. Oi R, Yamashita T, Takeda Y, Nakayama S, Hatakeyama M, Maruyama T, Sakamoto H, Adachi T, Okai
T, Kubota T, Nakabayashi M. Indication and detective rate of amniocentesis changed by the adoption of
non-invasive prenatal genetic testing. 13th International Congress of Human Genetics 2016.04.04 Kyoto
2. Hiraoka K, Hayashi N, Kaji T, Yamaguchi K, Ichihashi A, Suhara T,Ohtsuka Y, Kidera N, Ishikawa T,
Ohuchi K, Takagi K. Can Piezo-ICSI improve the fertilization rates of junior embryologists and how many
procedures are needed?. . The 6th Congress of the Asia Paciﬁc Initiative on Reproduction 2016.04.09
3. Masakazu Terauchi. Palpitation in middle-aged women is associated with anxiety. 68th Annual Congressof Japan Society of Obstetrics and Gynecology 2016.04.22
4. Ishikawa T, Kubota T. 27-hydroxycholesterol promotes estrogen receptor positive breast cancer. 68th
Annual Congress of Japan Society of Obstetrics and Gynecology, Tokyo 2016.04.22
5. Kuwahara A, Ishikawa T, Ishihara O, Kugu K, Sawa R, Banno K, Saito H,
Irahara M. Assisted
Reproductive Technology（ART）in Japan 2013. Annual report of Japan ART registry. 68th Annual
Congress of Japan Society of Obstetrics and Gynecology 2016.04.22 Tokyo
6. Akihiko Ueda, Makoto Akiyama, Takashi Nakasuji, Shota Nii. Cesarean section in Japan : Epidemiology,
trends, and surgical procedures. 68th Annual Congress of Japan Society of Obstetrics and Gynecology
2016.04.23 Tokyo
7. Nakasuji T, Sakumoto T, Araki R, Kuwahara A, Kubota T. Saito H. The Incidence of Monozygotic
Twinning in Assisted Reproductive Technology in Japan. 2016ACOG Annual Clinical and Scientiﬁc
Meeting 2016.05 Washington DC, USA
8. Masakazu Terauchi, Asuka Hirose, Mihoko Akiyoshi, Toshiro Kubota. Palpitation in Middle-aged WomenIs Associated with Anxiety. 18th International Society of Psychosomatic Obstetrics and Gynecology
Congress 2016.05.12 Malaga, Spain
9. Asuka Hirose, Masakazu Terauchi, Mihoko Akiyoshi, Yoko Owa, Kiyoko Kato, Toshiro Kubota. Subjective insomnia is associated with low sleep eﬃciency and feeling of fatigue in middle-aged women.. 18th
International Society of Psychosomatic Obstetrics and Gynecology Congress 2016.05.13
10. Furusawa A ,Inoue J, Miyasaka N, Inazawa J. Diﬀerential requirement of amino acids on cell survival ofovarian cancer cells . 3rd Annual Meeting of the International Ovarian Cancer Consortium in conjuction
with the International Symposium of Tumor Microenvironment and Therapy Resistance 2016.08.29
11. Masakazu Terauchi, Asuka Hirose, Mihoko Akiyoshi, Makoto Iizuka, Naoyuki Miyasaka, Toshiro Kubota.
Palpitation in middle-aged women is associated with anxiety. 15th World Congress on the Menopause
2016.09.30 Prague, Czech Republic
12. Yamaguchi A, Iizuka M, Ohkura Y, Obayashi S. Functional change in rat tail artery by Gamma-Oryzanol.
The North American Menopause Society 2016.10.07
13. Oshima N, Fusegi A, Furusawa A, Toba M, Wakana K, Wakabayashi A, Obayashi S. Adjuvant Therapy
After Radical Hysterectomy for Cervical Cancer. Biennial Meeting of the International Gynecologic Cancer
Society 2016.10.30― 350 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

14. Takahashi K, Kato T, Kobayshi M , Takehara Y, Tsukada T, Tate K, Shimizu H, Uehara T, Ishikawa M,
Ikeda S . Oncologic outcome and adverse events after para-aortic lymphadenectomy for FIGO stage IIIC
endometrioid adenocarcinoma of the uterine body. 16th Beinnial meeting of the International Gynecologic
Cancer Society, 2016.10.30
15. Kazuki Saito, Toshiya Matsuzaki, Mami Miyado, Momori Katsumi, Shigeru Nakamura, Minoru Irahara,
Tsutomu Ogata, Maki Fukami. Androgen measurement in women with polycystic ovary syndrome: Comparison between immunoassays and liquid chromatography-tandem mass spectrometry. APPES-JESPE
joint meeting 2016.11.18 Tokyo
16. Kawai K, Ohuchi K, Nara K, Miyagawa T, Kidera N, Iwahara Y, Yamamoto A, Ishikawa T, Kawahara M,
Teraoka K, Harada T. Eﬃcacy of Random-start Controlled Ovarian Stimulation in Breast Cancer Patients
Asian Society for Fertility Preservation.. The 1st Asia congress on fertility preservation 2016.11.19
17. Ishikawa T, Nonaka M, Murakata S, Kidera N, Yamamoto A, Iwahara Y, Miyasaka N. Fertility preservation at Tokyo Medical and Dental University: Our 1year experience Asian Society for Fertility Preservation. The 1st Asia congress on fertility preservation 2016.11.24― 351 ―

Systemic Organ Regulation

Systemic Organ Regulation

Urology

Professor and Chairman: Kazunori Kihara (-March), Yasuhisa Fujii (August-)
Associate Professor: Yasuhisa Fujii (-July)
Junior Associate Professor: Kazutaka Saito,
Noboru Numao (-February Department of Insured Medical Care Management),
Yoh Matsuoka, Junichiro Ishioka (-February, March- Department of Insured Medical Care Management)
Assistant Professor: Minato Yokoyama, Soichiro Yoshida, Toshiki Kijima (March-), Masaya Ito,
Masaharu Inoue
Hospital Staﬀ: Naoko Kawamura, Hajime Tanaka, Takayuki Nakayama, Shingo Moriyama (-March),
Yuma Waseda, Yuki Nakamura (-October), Motohiro Fujiwara (-June), Shugo Yajima (November-),
Keita Izumi (April-)
Graduate Student: Naoko Kawamura (-March), Sachi Kitayama, Toshihiro Kanda (-March), Saori Araki,
Yosuke Yasuda, Sho Uehara, Yuma Waseda, Hiroshi Fukushima, Shingo Moriyama (April-)
Project professor: Kazunori Kihara (April-)(1)

OutlineOur mission is to establish and provide the best urological care to all patients in the super aging society which
all over the world is facing.
Besides oﬀering urological practices of the international standard, we are making a continuous eﬀort to improvedaily practices based on the evidences of the clinical and translational research which we commit under theconcept of Bed to Bench, Feedback to Bed .
To realize the mission, we have been developing various procedures.
1. Gasless Single-Port RoboSurgeon urological surgery which can be applied to most of patients with urologicaltumors is a safe and educational minimally invasive surgery satisfying no CO2 gas insuﬄation, no peritoneal
injury, no multiple ports, and no high cost.
2. Bladder sparing treatment consisting of transurethral resection of bladder tumor, low-dose chemoradiotherapy
and Gasless Single-Port RoboSurgeon partial cystectomy with pelvic lymph node dissection can preserve urinaryand sexual function without compromising curability in carefully selected patients with muscle-invasive bladdercancer for which radical cystectomy with urinary diversion is the standard treatment.
3. Gasless Single-Port RoboSurgeon clampless partial nephrectomy for kidney cancer potentially preserves
maximal renal function with minimal invasiveness especially in patients with single kidney or impaired renal
function.
4. Focal brachytherapy for localized prostate cancer precisely diagnosed by MRI and meticulous biopsy canprovide cure without deteriorating urinary and sexual function.
The continuous commitment to clinical and translational research is reﬂected to publications in international
journals, presentations at international meetings and awards.

(2)

Research

Clinical Research
1) Innovation and establishment of a minimally invasive surgery, Gasless Single-Port RoboSurgeon urologicalsurgery2) Development of optimal MRI-ultrasonography fusion prostate needle biopsy― 352 ―

Systemic Organ Regulation

Systemic Organ Regulation

3) Sequential combination therapy to prolong survival of advanced prostate cancer patients
4) Development and establishment of curative and minimally invasive bladder preservation using low-dose
chemoradiotherapy plus Gasless Single-Port RoboSurgeon partial cyctectomy against muscle-invasive bladdercancer5) Development and establishment of Gasless Single-Port RoboSurgeon clampless partial nephrectomy against
kidney cancer6) Development and establishment of focal brachytherapy against localized prostate cancer
7) Sequential combination therapy to prolong survival of advanced kidney cancer patients, starting with immunotherapy combined with multiple molecular targeted agents
8) Application of diﬀusion-weighed MRI to diagnosis, assessment of therapeutic eﬀects and monitoring of relapse
in urological cancer
9) Application of serum C-reactive protein as a prognostic biomarker of urological malignancies and as a marker
for surgical invasiveness10) Development of prognostic prediction model for non-muscle-invasive bladder cancer
Translational Research1) Development of diﬀerentiation-inducing therapy against hormone-resistant prostate carcinomas
2) Investigation on molecular mechanisms, in particular deregulation of the NO system, underlying voiding anderectile dysfunction to develop rational therapy3) Overcoming therapeutic resistance to chemo- and/or radiotherapy against urological malignancies using novel
molecular targeted agents
4) Investigation on functional roles of p63 protein in urothelial carcinomas(3)

Lectures & Courses

Our top priority is to establish the best urological practice in the super aging society which all over the world isfacing. We are committed to oﬀering educational programs to facilitate the development of outstanding academic
urologists of the next generation. We believe that one of our missions is to educate students, residents and fellows
in the art and science of urology and thereby to train the future leaders in the ﬁeld. The continuous commitment
to clinical and translational research is reﬂected to publications in international journals, presentations atinternational meetings and awards.
(4)

Clinical Performances

Our mission is to provide the best urological care to all patients. Besides oﬀering urological practices of theinternational standard, we are making a continuous eﬀort to improve daily practices. The Gasless Single-Port
RoboSurgeon urological surgery, which we have innovated its concept and developed surgical techniques speciﬁc
to all urological organs, has been oﬃcially approved as medical services provided by the Japanese Governmental
Health Insurance System in April 2008. These minimally invasive surgical techniques can be fundamentally
applied to all patients having urological malignancies, even those having locally advanced disease and previous
histories of abdominal surgery.
(5)

Publications[Original Articles]
1. Madoka Kataoka, Hiroshi Fukushima, Yasukazu Nakanishi, Minato Yokoyama, Nobuaki Funata, Toru
Motoi, Ken-Ichi Tobisu, Fumitaka Koga. Retroperitoneal Teratoma in an Adult: A Potential Pitfall in
the Diﬀerential Diagnosis of Adrenal Myelolipoma. Case Rep Urol. 2016; 2016; 5141769
2. Yoh Matsuoka, Noboru Numao, Kazutaka Saito, Hiroshi Tanaka, Jiro Kumagai, Soichiro Yoshida, Junichiro Ishioka, Fumitaka Koga, Hitoshi Masuda, Satoru Kawakami, Yasuhisa Fujii, Kazunori Kihara.
Candidate selection for quadrant-based focal ablation through a combination of diﬀusion-weighted magnetic resonance imaging and prostate biopsy. BJU Int.. 2016.01; 117(1); 94-101
3. Hajime Tanaka, Soichiro Yoshida, Yoshinobu Komai, Yasuyuki Sakai, Shinji Urakami, Takeshi Yuasa,
Shinya Yamamoto, Hitoshi Masuda, Mitsuru Koizumi, Atsushi Kohno, Iwao Fukui, Junji Yonese, Ya-― 353 ―

Systemic Organ Regulation

Systemic Organ Regulation

suhisa Fujii, Kazunori Kihara. Clinical Value of 18F-Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography in Upper Tract Urothelial Carcinoma: Impact on Detection of Metastases
and Patient Management. Urol. Int.. 2016.01; 96(1); 65-72
4. Takayuki Nakayama, Noboru Numao, Soichiro Yoshida, Junichiro Ishioka, Yoh Matsuoka, Kazutaka Saito,
Yasuhisa Fujii, Kazunori Kihara. A novel interactive educational system in the operating room–the IE
system. BMC Med Educ. 2016.02; 16; 44
5. Naoko Kawamura, Minato Yokoyama, Yasuhisa Fujii, Junichiro Ishioka, Noboru Numao, Yoh Matsuoka,
Kazutaka Saito, Chizuru Arisawa, Tetsuo Okuno, Akira Noro, Shinji Morimoto, Kazunori Kihara. Recovery of renal function after radical nephrectomy and risk factors for postoperative severe renal impairment:
A Japanese multicenter longitudinal study. Int. J. Urol.. 2016.03; 23(3); 219-223
6. Masanori Murakami, Takanobu Yoshimoto, Yujiro Nakano, Kyoichiro Tsuchiya, Isao Minami, Ryotaro
Bouchi, Yasuhisa Fujii, Kazuhiko Nakabayashi, Koshi Hashimoto, Ken-Ichiro Hata, Kazunori Kihara,
Yoshihiro Ogawa. Expression of inﬂammation-related genes in aldosterone-producing adenomas withKCNJ5 mutation. Biochem. Biophys. Res. Commun.. 2016.08; 476(4); 614-619
7. Toshihiro Kanda, Syohei Fukuda, Naotaka Fukui, Yu Ohkubo, Tomoko Kazumoto, Yoshihiro Saito, Ayataka Ishikawa, Masafumi Kurosumi, Yukio Kageyama, Yasuhisa Fujii, Kazunori Kihara. Favorable
outcome of intraoperative radiotherapy to the primary site in patients with metastatic prostate cancer.
Int. J. Clin. Oncol.. 2016.08; 21(4); 764-772
8. Kawano S, Komai Y, Ishioka J, Sakai Y, Fuse N, Ito M, Kihara K, Saito N.. Development of prognostic
model for predicting survival after retrograde placement of ureteral stent in advanced gastrointestinal cancer patients and its evaluation by decision curve analysis. MINERVA UROLOGICA E NEFROLOGICA.
2016.10; 68(5); 437-443
9. Mutsushi Yamasaki, Takeshi Yuasa, Sho Uehara, Yasuhisa Fujii, Shinya Yamamoto, Hitoshi Masuda,
Iwao Fukui, Junji Yonese. Improvement of renal function by changing the bone-modifying agent fromzoledronic acid to denosumab. Int. J. Clin. Oncol.. 2016.12; 21(6); 1191-1195[Misc]
1. Kazutaka Saito. Words of Wisdom. Re: Nephron-sparing Techniques Independently Decrease the Riskof Cardiovascular Events Relative to Radical Nephrectomy in Patients with a T1a-T1b Renal Mass andNormal Preoperative Renal Function. Eur. Urol.. 2016.03; 69(3); 538
2. Kazutaka Saito. Words of Wisdom. Re: Evaluation of Functional Outcomes After Laparoscopic Partial
Nephrectomy Using Renal Scintigraphy: Clamped vs. Clampless Technique. Eur. Urol.. 2016.04; 69(4);753
3. Minato Yokoyama. Editorial Comment to Outcomes of artiﬁcial urinary sphincter placement in octogenarians. Int. J. Urol.. 2016.05; 23(5); 423-424
4. Kazutaka Saito. Local Treatment of the Prostate in Metastatic Prostate Cancer: Need to Change theConcept? Eur. Urol.. 2016.06;
5. Matsuoka Y, Inoue M, Ito M, Yoshida S, Yokoyama M, Ishioka J, Numao N, Saito K, Fujii Y, Kihara K.
Gassless single-port RoboSurgeon radical prostatectomy using an endoscopic manipulator robot 2016.07;
8(1); 31-35
6. Matsuoka Y, Inoue M, Ito M, Yoshida S, Yokoyama M, Ishioka J, Numao N, Saito K, Fujii Y, Kihara K.
Gassless single-port RoboSurgeon radical prostatectomy using an endoscopic manipulator robot 2016.07;
8(1); 31-35
7. Kazutaka Saito. Reply to Umberto Capitanio, Paolo Dell’Oglio, Alessandro Larcher, and Francesco
Montorsi’s Reply to Kazutaka Saito’s Words of Wisdom re: Nephron-sparing Techniques Independently
Decrease the Risk of Cardiovascular Events Relative to Radical Nephrectomy in Patients with a T1a-T1b
Renal Mass and Normal Preoperative Renal Function. Eur Urol. 2016;69:538. Eur. Urol.. 2016.10; 70(4);e99― 354 ―

Systemic Organ Regulation

Systemic Organ Regulation[Conference Activities & Talks]

1. Saito K. Focal Brachytherapy for Localized Prostate Cancer . 5th Metropolitan Urology Seminar2016.01.23 Tokyo, Japan
2. Yoshida S et al. . Potential utility of standardized apparent diﬀusion coeﬃcient value as a biomarker
predicting clinical aggressiveness of bladder cancer with various MRI protocols at various
institutions. .European Association of Urology annual congress 2016.03.12 Munich, Germany
3. Tanaka H et al. Novel stepwise algorithm using CT and MRI for diﬀerential diagnosis of fat-poor angiomyolipoma in small renal masses: Development and external validation. . European Association of
Urology annual congress 2016.03.12 Munich, Germany
4. Ishioka J et al. When should patients undergo prostate biopsy? Decision analysis using diﬀerences in
the health-related quality-of-life between pre-biopsy healthy men and patients with castration-resistant
prostate cancer. .European Association of Urology annual congress 2016.03.12 Munich, Germany
5. Fujiwara M et al.. Antibiotic prophylaxis is avoidable in minimally invasive clean surgery for renal or
adrenal tumors: A prospective study of 678 cases. Munich, Germany 2016.03.12 Munich, Germany
6. 1.Fujii Y, Kihara K, Tanaka H, Saito K., Yoshida S, Yokoyama M., Ishioka J, Matsuoka Y, Numao N.
. Bladder-sparing protocol consisting of ow-dose chemoradiotherapy and consolidative partial cystectomy
against muscle-invasive bladder cancer: Oncological and functional outcome in elderly patients..
European Association of Urology annual congress 2016.03.13 Germany
7. 5.Yokoyama M, Kawamura N, Fujii Y, Inoue M, Ishioka J, Numao N, Matsuoka Y, Saito K, Arisawa
C, Okuno T, Noro A, Morimoto S, Kihara K. . Longitudinal changes in renal function after radical
nephrectomy and risk factors for postoperative severe renal impairment: A Japanese multicenter study
using a linear mixed model analysis. European Association of Urology annual congress 2016.03.13 Germany
8. Moriyama S et al. A new quantitative method for characterizing small renal masses: MRI intensity ratio
curve analysis. European Association of Urology annual congress 2016.03.13 Munich, Germany
9. Waseda Y. et al.. Elevation of urinary metanephrine/normetanephrine and impairment of glucose
toler-ance predict the development of hypoglycemia after resection of pheochromocytoma.
European Association of Urology annual congress 2016.03.13
10. Nakamura Y et al. Renal dysfunction manifestation in patients with adrenal Cushing’s syndrome after
adrenalectomy. European Association of Urology annual congress 2016.03.13 Munich, Germany
11. Masaharu Inoue, Fumitaka Koga, Soiciro Yoshida, Hajime Tanaka, Shuichiro Kobayashi, Masaya Ito,
Minato Yokoyama, Junichiro Ishioka, Yoh Matsuoka, Noboru Numao, Kazutaka Saito, Yasuhisa Fujii,
Kazunori Kihara. Prediction of therapeutic resistance using ERBB2 expression status and Ki-67 labelingindex in muscle-invasive bladder cancer patients treated with chemoradiation-based selective bladdersparing approach. the 31st Annual EAU Congress 2016.03.14
12. 4.Kawamura N, Yokoyama M, Fujii Y, Inoue M, Ito M, Yoshida S, Ishioka J, Numao N, Matsuoka Y,
Saito K, Kihara K.. Longitudinal preservation of renal function after clamp-less partial nephrectomy: An
analysis of patients with chronic kidney disease.. European Association of Urology annual congress2016.03.14 Germany
13. 14.Yasuda Y, Saito K, Kawamura N, Yuasa T, Yokoyama M, Matsuoka Y, Ishioka J, Numao N, Okuno
T, Yamamoto S, Takahashi S, Yonese J, Fujii Y, Kihara K. Prognostic signiﬁcance of early CRP response
for metastatic renal cell carcinoma treated with tyrosine kinase inhibitor. European Association of
Urology annual congress 2016.03.14 Germany
14. Matsuoka Y, Ishioka J, Tanaka H, inoue M, Ito M, Yoshida S, Yokoyama M, Numao N, Saito K, Fujii Y,
Kihara K. Impact of PI-RADS version 2 on MRI diagnosis for extracapsular extension of prostate cancer:
A multireader study. European Association of Urology annual congress 2016.03.14 Munich, Germany― 355 ―

Systemic Organ Regulation

Systemic Organ Regulation

15. Yoshida S et al. The utility of diﬀusion-weighted MRI as an imaging biomarker of upper urinary tract
cancer: A preoperative prognostic indicator reﬂecting histological grade. . European Association of Urology
annual congress 2016.03.14 Munich, Germany
16. Inoue M et al. . Prediction of therapeutic resistance using ERBB2 expression status and Ki-67 labelling
index in muscle-invasive bladder cancer patients treated with chemoradiation-based selective bladdersparing approach. . European Association of Urology annual congress 2016.03.14 Munich, Germany
17. Matsuoka Y, Numao N, Saito K, Tanaka H, Inoue M, Ito M, Yoshida S, Yokoyama M, Ishioka J, Fujii
Y, Kihara K. Applicability analysis of focal therapy to intermediate- and high-risk prostate cancer. .
annual meeting of the American Urological Association 2016.05.06 San Diego, USA
18. Waseda Y et al. Impact of elevated urinary metanephrine/normetanephrine and impaired glucose tolerance
test on the development of hypoglycemia following removal of pheochromocytoma.
annual meeting of the American Urological Association 2016.05.06 San diego
19. Numao N et al. Optimal combination of MRI-targeted biopsy and systematic biopsy for men with suspicionof prostate cancer. . annual meeting of the American Urological Association 2016.05.06 San diego, USA
20. Ishioka J et al.. The incidence of late bladder tumor recurrence of upper tract urothelial carcinoma andoptimal cystoscopic follow-up: A multicenter study.. annual meeting of the American Urological
Association 2016.05.06 San diego, USA
21. Ishioka J et al. Prognostic impact of C-reactive protein kinetics on survival of patients with advanced
urothelial carcinoma. A multicenter study. . annual meeting of the American Urological Association
2016.05.06 San diego, USA
22. Nakamura Y et al.. Masked renal dysfunction in patients with adrenal Cushing’s syndrome manifested
by adrenalectomy. annual meeting of the American Urological Association 2016.05.06 San diego, USA
23. Nakamura Y et al. . High speciﬁcity of diﬀusion-weighted MRI in diﬀerentiating residual bladder cancerfrom postoperative changes. annual meeting of the American Urological Association 2016.05.06 San diego,USA
24. 21.Nakanishi Y, Fukushima H, Yokoyama M, Kataoka M, Tobisu K, Koga F. Can systematic biopsy be
safely omitted in the era of MRI/TRUS-fusion targeted prostate biopsy?. annual meeting of theAmerican Urological Association 2016.05.06 San diego, USA
25. Yoshida S et al. . Apparent diﬀusion coeﬃcient as a prognostic biomarker of upper urinary tract
cancer..annual meeting of the American Urological Association 2016.05.07 San diego, USA
26. Tanaka H et al. Development and validation of a novel stepwise algorithm using CT and MRI for
diagnosis of fat-poor angiomyolipoma in small renal masses. . annual meeting of the American Urological
Association 2016.05.07
27. Ito M et al. Likelihood of underlying Lynch syndrome in Japanese upper tract urothelial carcinoma
patients. annual meeting of the American Urological Association 2016.05.07 San diego, USA,
28. Ito M et al. Likelihood of underlying Lynch syndrome in Japanese upper tract urothelial carcinoma
patients. . San diego, USA 2016.05.07
29. Kobayashi M et al. Maximum standardized uptake value of 18F-FDG PET/CT predicts survivalof patients with advanced urothelial carcinoma. annual meeting of the American Urological Association
2016.05.07 San diego, USA
30. Fujiwara M et al.. Feasibility of non-use of antimicrobial prophylaxis in minimally invasive. annual
meeting of the American Urological Association 2016.05.07 San diego, USA― 356 ―

Systemic Organ Regulation

Systemic Organ Regulation

31. Fukushima H, Nakanishi Y, Kataoka M, Tobisu K, Koga F. Prognostic signiﬁcance of postoperativeskeletal muscle mass recovery in metastatic renal cell carcinoma patients who underwent cytoreductive
nephrectomy. annual meeting of the American Urological Association 2016.05.07 San diego, USA
32. Fujii Y et al.. Bladder-sparing protocol consisting of low-dose chemoradiotherapy and consolidative partial
cystectomy against muscle-invasive bladder cancer: Comparison of oncological and functional outcomes
between older and younger patients. annual meeting of the American Urological Association
2016.05.08 USA
33. Matsuoka Y, Ishioka J, Tanaka H, Nakayama T, Inoue M, Ito M, Yoshida S, Yokoyama M, Numao N,
Saito K, Fujii Y, Kihara K. Impact of prebiopsy magnetic resonance imaging (MRI) on preoperative risk
stratiﬁcation for biochemical recurrence after radical prostatectomy. . annual meeting of the American
Urological Association 2016.05.08 San Diego, USA
34. Ishioka J et al. Prognostic impact of C-reactive protein kinetics on survival of patients with advanced
urothelial carcinoma. A multicenter study. . annual meeting of the American Urological Association
2016.05.08 San diego, USA
35. 8.Ishioka J et al. Yokoyama M., Kawamura N., Fujii Y., Inoue M., Ishioka J., Numao N., Matsuoka
Y., Saito K., Arisawa C., Okuno T., Noro A., Morimoto S., Kihara K.. Longitudinal renal function
recovery after radical nephrectomy and risk factors for postoperative severe renal impairment: a Japanesemulticenter study.. annual meeting of the American Urological Association 2016.05.08 San diego, USA
36. Matsuoka Y, Ishioka J, Tanaka H, Inoue M, Ito M, Yoshida S, Yokoyama M, Numao N, Saito K, Fujii Y,
Kihara K. Impact of the Prostate Imaging Reporting and Data System version 2 (PI-RADS v2) on theassessment of extracapsular extension in prostate cancer: A multireader study.. annual meeting of theAmerican Urological Association 2016.05.09 San Diego, USA
37. Moriyama S et al. MRI intensity ratio curve analysis: a new objective method for characterizing small
renal masses.. annual meeting of the American Urological Association 2016.05.09 San diego, USA
38. 13.Nakayama T, Kazutaka S, Fujii Y, Waseda Y, Moriyama S, Tanaka H, Kawamura N, Inoue M, Ito M,
Yoshida, S, Yokoyama M, Matsuoka Y, Numao N, Kihara K. Association of M2 macrophage invasion
with C-reactive protein in renal cell carcinoma.. annual meeting of the American Urological Association
2016.05.09 San diego, USA
39. Masaharu Inoue, Fumitaka Koga, Soichiro Yoshida, Hajime Tanaka, Shuichiro Kobayashi, Minato Yokoyama,
Junichiro Ishioka, Yoh Matsuoka, Noboru Numao, Kazutaka Saito, Yasuhisa Fujii, Kazunori Kihara.
Predictive model for therapeutic resistance using ERBB2 expression status and Ki-67 labeling index inmuscle-invasive bladder cancer patients treated with chemoradiotherapy-based bladder-sparing protocol.
AUA2016 Annual Meeting 2016.05.09 San Diego, USA
40. S. Yoshida, F. Koga, H. Tanaka, H. Fukushima, Y. Nakanishi, M. Yokoyama, J. Ishioka, Y. Matsuoka, N.
Numao, K. Saito, Y. Fujii, K. Kihara.. Apparent diﬀusion coeﬃcient ratio as a potential inter-institutional
marker for histological grade of bladder cancer. 24th International Society of Magnetic Resonance in
Medicine (ISMRM) 2016.05.12 Singapore
41. Matsuoka Y, Saito K, Numao N, Tanaka H, Inoue M, Ito M, Yoshida S, Yokoyama M, Ishioka J, Fujii
Y, Kihara K. Risk assessment for undertreatment in focal therapy and its applicability to intermediateand high-risk prostate cancer. 9th International Symposium on Focal Therapy and Imaging in prostate& kidney cancer 2016.06.23 Durham, USA
42. Saito K, Matsuoka Y, Numao N, Hayashi K, Toda K, Ito M, Inoue M, Yoshida S, Yokoyama M, Ishioka J,
Fujii Y, Yoshimura R, Kihara K. Hemi-gland brachytherapy for localized prostate cancer. 9th International
Symposium on Focal Therapy and Imaging in prostate & kidney cancer 2016.06.23 Durham, USA
43. Ito M et al.. Likelihood of underlying Lynch syndrome in Japanese upper tract urothelial carcinoma
patients. . annual meeting of the American Urological Association San diego, USA― 357 ―

Systemic Organ Regulation

Systemic Organ Regulation[Awards & Honors]
1. Best Poster Presentation, The 31th Annual Congress of the European Association of Urology, European
Association of Urology, 2016.03
2. Best Poster Presentation, The 31th Annual Congress of the European Association of Urology, European
Association of Urology, 2016.03
3. Best Poster Presentation, The 31th Annual Congress of the European Association of Urology, European
Association of Urology, 2016.03
4. Best Poster Presentation, 110th annual meeting of the American Urological Association, American Urological Association , 2016.05― 358 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

Gastrointestinal Surgery

Professor
Project Professor
Masahiro TSUBAKI
Associate Professor
Masamichi YASHUNO, Mikito INOKUCHI, Yasuaki NAKAJIMA
Junior Associate Professor
Kenro KAWADA, Takuya OKADA
Assistant Professor
Yutaka TOKAIRIN, Akifumi KIKUCHI,Toshiro TANIOKA
Akihiro HOSHINO, Shinichi YAMAUCHI
Graduate Student
Yasunori SOMENO, Masafumi OKUDA, Taichi OGOU,
Michiyo TOKURA, Marie HANAOKA, Yuichiro KUME,
Yutaka NAKAJIMA, Toshihiro MATSUI, Tomoki ABURATANI,
Chiharu TOMII, Keisuke OKUNO, Kentaro GOKITA,
Fukuichiro ORITA, Yudai KAWAMURA, Tomiyuki MIURA,
Kazuya YAMAGUCHI, Megumi SASAKI, Rei GHO, Mora ANDRES(1)

Research

1) Development of esophageal surgery.
2) Development of gastric surgery.
3) Development of colorectal surgery.

(2)

Education

The history of the department started as both the Department of Esophageal and General Surgery and theDepartment of Surgical Oncology of TMDU, and many surgeons and researchers in various specialties havegathered and have been keeping a high level of activities. Our main purposes of education are to make thepost-graduate physicians grown up to excellent surgeons and to contribute in development of medical/surgicalsciences. Surgeons with high-level medical knowledge and techniques are expected to grow up in this department.
Moreover, making surgeons with matured humanity is one of the purposes. The department has a peacefulatmosphere and stands for active work in solving diﬃcult problems.

(3)

Clinical Performances

Main clinical services are diagnosis and treatment for esophageal, gastric and colorectal diseases. Postgraduate students learn and study general surgery and sub-specialty, e.g. esophageal surgery, gastric surgery
and colorectal surgery. The territory of clinics is wide and the department provides a full spectrum of standard
and special technologies such as minimally invasive surgery and extended radical surgery for malignancies.

― 359 ―

Systemic Organ
Organ Regulation
Regulation
Systemic(4)

Publications[Original Articles]
1. Okada T, Tanaka K, Kawachi H, Ito T, Nishikage T, Odagaki T, Zárate AJ, Kronberg U, López-Köstner
F, Karelovic S, Flores S, Estela R, Tsubaki M, Uetake H, Eishi Y, Kawano T. International collaboration
between japan and chile to improve detection rates in colorectal cancer screening. Cancer. 2016; 1(122);
71-77
2. Miyawaki Y, Nakajima Y, Kawada K, Okada T, Tokairin Y, Kawano T.. Eﬃcacy of docetaxel, cisplatin,
and 5-ﬂuorouracil chemotherapy for superﬁcial esophageal squamous cell carcinoma. Dis Esophagus.
2016;
3. Ishibashi T, Ishikawa S, Suzuki A, Miyawaki Y, Kawano T, Makita K.. Successful Anesthesia Management for 2-Stage Surgical Procedure of a Refractory Tracheogastric Tube Fistula After Esophagectomy.
A A Case Rep. 2016; 6(4); 84-87
4. Yutaka Tokairin, Yasuaki Nakajima, Kenro Kawada, Toru Kawamura, Tatsuyuki Kawano and Akira
Nalashima. An evaluation of the diagnostic potential of CT scans and herniography for inguinal hernias
Int Surg. 2016;
5. Yutaka Tokairin, Yasuaki Nakajima, Kenro Kawada, Akihiro Hoshino, Kagami Nagai and Tatsuyuki
Kawano. The usefulness of a long forceps technique in single-incision laparoscopic surgery for totally
extraperitoneal repair of inguinal hernia Int Surg. 2016;
6. Kumagai Y,Takubo K,Kawada K,Higashi M,Ishiguro T,Sobajima J,Fukuchi M,Ishibashi K,Mochiki E,Aida
J,Kawano T,Ishida H.. Endocytoscopic observation of various types of esophagitis Esophagus. 2016; 13;200-207
7. Kumagai Y,Sobajima J,Higashi M,Ishigurro T,Fukuchi M,Ishibashi K,Mochiki E,Yakabi K,Kawano T,Tamaru
j,Ishida H. Tumor-asociated macrophages and angiogenesis in early stage esophagneal squamous cell carcinoma Esophagus. 2016; 13; 245-253
8. Nakajima Y, Kawada K, Tokairin Y, Miyawaki Y, Okada T, Miyake S, Kawano T.. Retrospective Analysesof Esophageal Bypass Surgery for Patients with Esophagorespiratory Fistulas Caused by Esophageal
Carcinomas. World J Surg. 2016; 40(5); 1158-1164
9. Nakajima Y, Kawada K, Tokairin Y, Tomita T, Satoshi M, Kawano T.. Prognostic factors for postrecurrence survival in patients with thoracic esophageal squamous cell carcinoma after curative resection.
Dig Surg. 2016; 33(2); 136-145
10. Nakajima Y, Kawada K, Tokairin Y, Hoshino A, Miyawaki Y, Okada T, Miyake S, Kawano T.. Larynxpreserving surgery for cervical esophageal carcinoma can preserve the vocal function and improve theclinical outcome. Esophagus. 2016;
11. Nishikawaji T, Akiyama Y, Shimada S, Kojima K, Kawano T, Eishi Y, Yuasa Y, Tanaka S.
. Oncogenic roles of the SETDB2 histone methyltransferase in gastric cancer. Oncotarget. 2016; 7(41); 6725167265
12. IshiharaR,Oyama T, Abe S,Takahashi H, Ono H, Fujisaki J,Kaise M, Goda K, Kawada K,Koike T,Takeuchi
M, Matsuda R,Hirasawa D, Yamada M,Kodaira J,Tanaka M,Omae M,Matsui A,Kanesaka T,Takahashi
A,Hirooka S,Saito M,Tsuji Y,Maeda Y,Yamashita H, Oda J,Tomita Y,Matsunaga T,Terai S,Ozawa S,Kawano
T, Seto Y. Risk of metastasis in adenocarcinoma of the esophagus a multicencrt retrospective study in ajapanese population J Gastroenterol. 2016;
13. Takashi Ito, Hiroshi Kawachi, Paulina Peñaloza, Alejandro José Zárate,Alejandra Ponce, Maki Kobayashi,
Takuya Okada, Tomoyuki Odagak1,Koji Tanaka, Tetsuro Nishikage, Udo Kronberg, Masahiro Tsubaki,Yoshinobu Eishi, Tatsuyuki Kawano, Francisco López-Köstner. Protocolo Estandarizado de Anatomía
Patológica de lesiones polipoideas en Proyecto de Prevención de Neoplasias Colorrectales (PRENEC) en
Chile. Gastroenterol. Latinoam. 2016; 27; 37-46― 360 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

14. Takashi Ito, Hiroshi Kawachi, Paulina Peñaloza, Alejandro José Zárate,Alejandra Ponce, Maki Kobayashi,
Takuya Okada, Tomoyuki Odagak1,Koji Tanaka, Tetsuro Nishikage, Udo Kronberg, Masahiro Tsubaki,Yoshinobu Eishi, Tatsuyuki Kawano, Francisco López-Köstner. Protocolo Estandarizado de Anatomía
Patológica de lesiones polipoideas en Proyecto de Prevención de Neoplasias Colorrectales (PRENEC) en
Chile. Gastroenterol. Latinoam. 2016; 27; 37-46
15. T Muramatsu, K-i Kozaki, S Imoto6, R Yamaguchi, H Tsuda, T Kawano, N Fujiwara, M Morishita,
S Miyano and J Inazawa. The hypusine cascade promotes cancer progression and metastasis throughthe regulation of RhoA in squamous cell carcinoma Oncogene (2016), 1–13 Oncogene advance online
publication. 2016;
16. Nakajima Y, Kawada K, Tokairin Y, Tomita M, Miyake S, Kawano T. Prognostic Factors for PostRecurrence Survival in Patients with Thoracic Esophageal Squamous Cell Carcinoma after Curative Resection. Dig Surg (Epub ahead of print).
17. Nakajima Y, Kawada K, Tokairin Y, Miyawaki Y, Okada T, Satoshi Miyake, Kawano T. Retrospective Analyses of Esophageal Bypass Surgery for Patients with Esophagorespiratory Fistulas Caused by
Esophageal Carcinomas. World J Surg (Epub ahead of print).
[Conference Activities & Talks]
1. Nakajima Y, Kawada K, Tokairin Y, Chiba T, Hoshino A, Miyawaki Y, Okada T, Kawano T.. Super-highlarynx-preserving surgery for cervical esophageal carcinoma.. 51st Congress of the European Society for
Surgical Research 2016.05.28 Prague
2. Nakajima Y, Kawada K, Tokairin Y, Hoshino A, Okada T, Ogo T, Okuda M, Kawamura Y, Yamaguchi
K, Kawano T. A pilot trial of S-1 and paclitaxel in relapsed or refractory esophageal squamous cell
carcinoma pre-treated by docetaxel, cisplatin and 5-ﬂuorouracil chemotherapy.. 26th World Congress of
the International Association of Surgeons, Gastroenterologists and Oncologists 2016.09.10 Seoul
3. Nakajima Y, Kawada K, Tokairin Y, Chiba T, Hoshino A, Okada T, Okuda M, Ogo T, Nakajima Y,
Matsui T, Kawano T.. Larynx-preserving surgery for cervical esophageal carcinomaextending aroundthe esophageal oriﬁce.. 15th WORLD CONGRESS International Society for Diseases of the Esophagus（ISDE） 2016.09.19 Singapore
4. Yutaka Tokairin，Yasuaki Nakajima，Kenro Kawada，Tetsuma Chiba，Akihiro Hoshino，Takuya Okada，Naoto Fujiwara，Yutaka Nakajima，Hisashi Fujiwara，Taichi Ogo，Masafumi Okuda，Yuichiro Kume，
Toshihiro Matsui，Kagami Nagai，Keiichi Akita，Tatsuyuki Kawano.. Anatomical feature during upper mediastinal lymph nodes dissection using a trans-bicervical approach under the pneumomediastinum.. 15th
WORLD CONGRESS International Society for Diseases of the Esophagus（ISDE） 2016.09.20 Singapore
5. Kenro Kawada. Long-term prognosis of esophageal squmoaus cell carcinoma invading muscularis mucosae:comparison between ER and radical surgery. 15th WORLD CONGRESS International Society for
Diseases of the Esophagus（ISDE） 2016.09.20 Singapore
6. Masafumi Okuda.. Mucoepidermoid carcinoma of the esophagus detected in minimal lesion.. 15th
WORLD CONGRESS International Society for Diseases of the Esophagus（ISDE） 2016.09.20 Singapore
7. Akihiro Hoshino.. The optimal wavelength for low-concentration iodine staining with ﬂexible spectral
imaging color enhancement for esophageal cancer screening.. 15th WORLD CONGRESS International
Society for Diseases of the Esophagus（ISDE） 2016.09.20 Singapore
8. Taichi Ogo, Kenro Kawda, Yasuaki Nakajima, Yutaka Tokairin, Tetsuma Chiba, Akihiro Hoshino, Takuya
Okada, Masafumi Okuda, Yutaka Nakajima, Toshihiro Matsui, Cho, Mora, Yudai Kawamura, Kazuya
Yamaguchi, Takashi Ito, Tatsuyuki Kawano. Analysis of avascular area of superﬁcial esophageal cancers
with magnifying endoscopy after formalin ﬁxation.. 15th WORLD CONGRESS International Society for
Diseases of the Esophagus（ISDE） 2016.09.20 Singapore
9. Yutaka Tokairin. Mediastinoscopic approach with bilateral cervico-hiatal pneumomediastinum in radical
esophagectomy.. 40th World Congress of the International College of Surgeons 2016.10.25 Kyoto― 361 ―

Systemic Organ
Organ Regulation
Regulation
Systemic

10. Hisashi Fujiwara, Yasuaki Nakajima, Kenro Kawada, Yutaka Tokairin, Masafumi Okuda, Taichi Ogo, Katsumasa Saito, Naoto Fujiwara, Tairou Ryoutokuji, Takuya Okada, Yutaka Miyawaki, Youichi Kumagai,
Kagami Nagai, Tatsuyuki Kawano. . Eﬃcacy of endoscopy for evaluating esophago-gastric anastomosis
on post-esophagectomy day 1. . 14th World Congress of the International Society for Diseases of theEsophagus― 362 ―

Systemic
Regulation
Systemic Organ
Organ Regulation

Thoracic Surgery

Professor Kenichi Okubo
Junior Associate Professor Hironori Ishibashi
Assistant Professor Masashi Kobayashi
Hospital assistant professor Chihiro Takasaki
Graduate Student
Sachiko Imai
Graduate Student
Akiko Sugawara
Graduate Student
Ken Takahashi
Graduate Student
Katsutoshi Seto
Graduate Student
Ryo Wakejima
Graduate Student
Syunichi Baba
Graduate Student
Yasuhiro Nakashima(1)

OutlineDepartment of Thoracic Surgery deal with clinical management, basic and clinical research, and education ofthoracic surgery, which includes surgical diagnosis and treatment of respiratory diseases.
(2)

Research· Minimally invasive surgery for lung cancer· Multimodal treatments for thoracic malignancies· Surgery for metastatic lung tumors· Clinico-pathological studies on lung cancer(3)

Education

Department of Thoracic Surgery has a mission to educate medical post-graduates for expert thoracic surgeons.
Thoracic surgeon requires the Board of Surgery and the Board of Thoracic Surgery to perform clinical cares asa specialist. We provide clinical specialty course for thoracic surgery and graduate course for thoracic surgery,and support to obtain the boards.
(4)

Clinical Services & Other Works

Out-patient Clinic: Tuesday, Thursday,Friday
Operative Day: Monday, Wednesday, Thursday, Friday
Clinical Conference: Monday
Chest Conference : Monday
Clinico-pathological Conference: Wednesday
Medical Round: every morning
Professor’s Round: Tuesday
Journal Club: Tuesday (every other week)
Mortality & Morbidity Conference: Tuesday (every other week)
Lab Meeting: Tuesday (monthly)― 363 ―

Systemic Organ
Organ Regulation
Regulation
Systemic

Scientiﬁc Meeting: Japan Surgical Society, Japanese Association for Chest Surgery, Japanese Association for
Thoracic Surgery, Japan Lung Cancer Society, Japan Society for Respiratory Endoscopy(5)

Clinical Performances

Thoracic Surgery deal with surgical treatment for lung, mediastinum, pleura and chest wall. We provide highgrade medical care as a university hospital. We oﬀer less invasive surgery for early-stage lung cancers or benign
diseases, and multimodality treatment for locally advanced thoracic malignancies.
(6)

Publications[Original Articles]
1. Iwata T, Yoshino I, Yoshida S, Ikeda N, Tsuboi M, Asato Y, Katakami N, Sakamoto K, Yamashita Y,
Okami J, Mitsudomi T, Yamashita M, Yokouchi H, Okubo K, Okada M, Takenoyama M, Chida M, Tomii
K, Matsuura M, Azuma A, Iwasawa T, Kuwano K, Sakai S, Hiroshima K, Fukuoka J, Yoshimura K, Tada
H, Nakagawa K, Nakanishi Y; West Japan Oncology Group. A phase II trial evaluating the eﬃcacy andsafety of perioperative pirfenidone for prevention of acute exacerbation of idiopathic pulmonary ﬁbrosis
in lung cancer patients undergoing pulmonary resection West Japan Oncology Group 6711 L (PEOPLE
Study). Respir Res.. 2016.07; 17(1); 90
2. Hironori Ishibashi, Masashi Kobayashi, Chihiro Takasaki, Seiji Ishikawa, Yutaka Miura, Koshi Makita,
Kenichi Okubo. Eﬃcacy of Supraglottic Airway for Preventing Lung Injury Associated with Coughing at
Extubation after Pulmonary Lobectomy. World Journal of Surgery. 2016.08; 40(8); 1892-1898
3. Chihiro Takasaki, Masashi Kobayashi, Hironori Ishibashi, Takumi Akashi, Kenichi Okubo. Expression of
hypoxia-inducible factor-1 aﬀects tumor proliferation and antiapoptosis in surgically resected lung cancer
Molecular and Clinical Oncology. 2016.08; 5(2); 295-300
4. Kobayashi M, Huang C. L., Sonobe M, Kikuchi R, & Date H. Ad-shWnt2b Vector Therapy Demonstrates
Antitumor Activity in Orthotopic Intrapleural Models as Monitored with the In Vitro Imaging System(IVIS). Anticancer Research. 2016.11; 36(11); 5887-5893
5. Sachiko K,Hironori Ishibashi,Ken Takahashi, Kenichi Okubo,. Transcervical excision of thymoma andvideo-assisted thoracoscopic extended thymectomy (VATET) for ectopic cervical thymoma with myasthenia gravis: report of a case General Thoracic and Cardiovascular Surgery . 2016.12; 64(12); 752-751
6. Tomii S, Akashi T, Ando N, Tamura T, Sakurai A, Terada A, Furukawa A, Suzuki Y, Kayamori K,
Sakamoto K, Ishibashi H, Eishi Y.. Cortical Actin Alteration at the Matrix-Side Cytoplasm in Lung
Adenocarcinoma Cells and Its Signiﬁcance in Invasion. Pathobiology.(in print). 2016.12;[Others]
1. Successful excision of a massive bleeding schwannoma by thoracoscopic surgery., 2016.04
Asian Cardiovascular & Thoracic Annals2016, Vol. 24(5) 484-486. Hironori Ishibashi, Chihiro Takasaki
and Kenichi Okubo
2. Preservation of the lung after 4-year compression by ﬁbrous tumors of pleura, 2016.06
Asian Cardiovascular & Thoracic Annals 2016, Vol. 24(7)718-721
Hironori Ishibashi, Ken Takahashi, Sachiko Kumazawa and Kenichi Okubo.

― 364 ―

Systemic Organ Regulation

Igakuken Disease-oriented Molecular Biology

Visiting Professor

Takahiko Hara

Visiting Professor

Masanari Itokawa

Visiting Professor

Masato Hasegawa

Visiting Professor

Haruo Okado

Graduate Student Mai

Kanokoda,

MarinoNakajima, Rena Takahashi, Natsumi Yokote,Narumi Ikeda (April~), Risa Kanda (April~),
Kasumi Kori (April~), Riichi Okuda (April~)(1) Research[Takahiko Hara]

We attempt to elucidate how tissue stem cells (hematopoietic stem

cells, skeletal muscle stem cells, etc.) are developed in embryos and maintained in
adults by utilizing in vitro differentiation systems of ES/iPS cells and conditional KO
mouse strains. In addition, we advance the molecular biology of CXCL14, which is
involved in obesity-induced diabetes, carcinogenesis, feeding behavior, etc.
[Masanari Itokawa]

Our research focuses on unraveling the pathophysiology ofmental illnesses using molecular biology tools. Our ultimate goal is to identify new
disease mechanisms, leading to the development of novel and more efficacious
therapies. We perform genetic association studies, as well as metabolomics studies
using samples from patients with mental disorders. Any abnormalities identified
from patient samples are investigated further, using in vitro and in vivo systems,
such as, cell culture assays to highlight functional alterations and behavioral studies
in gene knockout mouse models.
[Masato Hasegawa]We investigate the molecular pathogenesis and progression ofneurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease andamyotrophic lateral sclerosis. We use biochemistry, immunohistochemistry andmolecular biology in all our work of in vitro, cellular and animal models to findeffective ways for clinical therapy.
[Haruo Okado]

To discover the fundamental cause of various nervous diseases, e.g.,

brain tumors, brain malformations, and neurodevelopmental disorders, we will study
the molecular mechanisms for the regulation of neural development in the cerebral
cortex using gene-targeted mice, primary cultures, viral vectors, in-utero― 365 ―

Systemic Organ Regulationelectroporation, real-time imaging of slice culture, immunohistochemistry, andtranscription analysis.

(2) Education
We will educate students for the purpose that they could investigate molecularmechanisms of life-threatening diseases such as cancer, diabetes, schizophrenia,
amyotrophic lateral sclerosis, and brain malformations. Trained students will
eventually help us to develop novel therapeutic strategies against them. In addition,
they must learn the importance of good animal models (including genetically engineeredmice), which faithfully reproduce symptom and progression of the diseases.

(3) Publications[Original Articles]
1.

M. Kawaguchi, K. Kitajima, M. Kanokoda, H. Suzuki, K. Miyashita, M. Nakajima,
H. Nuriya, K. Kasahara, and T. Hara. Efficient production of platelets from mouseembryonic stem cells by enforced expression of Gata2 in late hemogenic endothelial
cells. Biochem. Biophys. Res. Commun., 474: 462-468, 2016.

2.

K. Tamura, H. Naraba, T. Hara, K. Nakamura, M. Yoshie, H. Kogo, and E.
Tachikawa. A positive feedback loop between progesterone and microsomal
prostaglandin E synthase-1-mediated PGE2 promotes production of both in mouse
granulosa cells. Prostaglandins Other Lipid Mediat., 123: 56-62, 2016.

3.

T. Suzuki, Y. Kazuki, M. Oshimura, and T. Hara. Highly efficient transfer ofchromosomes to a broad range of target cells using Chinese hamster ovary cells
expressing murine leukemia virus-derived envelope proteins. PLoS ONE, 11:e0157187, 2016.

4.

K. Tanegashima, Y. Sato-Miyata, M. Funakoshi, Y. Nishito, T. Aigaki, and T. Hara.
Epigenetic regulation of the glucose transporter gene Slc2a1 by b-hydroxybutyrate
underlies preferential glucose supply to the brain of fasted mice. Genes Cells, 22:71-83, 2017.

5.

H. Ohtsuka, T. Iguchi, M. Hayashi, M. Kaneda, K. Iida, M. Shimonaka, T. Hara, M.
Arai, Y. Koike, N. Yamamoto, and K. Kasahara. SDF-1ǂ/CXCR4 signaling in lipid
rafts induces platelet aggregation via PI3 kinase-dependent Akt phosphorylation.

PLoS ONE, 12: e0169609, 2017.
6.

R. Mizutani, R. Saiga, S. Takekoshi, C. Inomoto, N. Nakamura, M. Itokawa, M.

― 366 ―

Systemic Organ Regulation

Arai, K. Oshima, A. Takeuchi, K. Uesugi, Y. Terada, and Y. Suzuki. A method for
estimating spatial resolution of real image in the Fourier domain. J Microsc., 261:
57-66. 2016.
7.

I. Kushima, B. Aleksic, M. Nakatochi, T. Shimamura, T. Shiino, A. Yoshimi, H.
Kimura, Y. Takasaki, C. Wang, J. Xing, K. Ishizuka, T. Oya-Ito, Y. Nakamura, Y.
Arioka, T. Maeda, M. Yamamoto, M. Yoshida, H. Noma, S. Hamada, M. Morikawa, Y.
Uno, T. Okada, T. Iidaka, S. Iritani, T. Yamamoto, M. Miyashita, A. Kobori, M. Arai,
M. Itokawa, M.-C. Cheng, Y.-A. Chuang, C.-H. Chen, M. Suzuki, T. Yoshikawa, S.
Numata, T. Ohmori, S. Kunimoto, D. Mori, N. Iwata, and N. Ozaki. High-resolution
copy number variation analysis of schizophrenia in Japan. Molecular Psychiatry,doi:10.1038/mp.2016.88 published online 2016.

8.

M. Miyashita, T. Watanabe, T. Ichikawa, K. Toriumi, Y. Horiuchi, A. Kobori, I.
Kushima, R. Hashimoto, M. Fukumoto, S. Koike, H. Ujike, T. Arinami, Y.
Tatebayashi, K. Kasai, M. Takeda, N. Ozaki, Y. Okazaki, T. Yoshikawa, N. Amano,
S. Washizuka, H. Yamamoto, T. Miyata, M. Itokawa, Y. Yamamoto, and M. Arai.
The regulation of soluble receptor for AGEs contributes to carbonyl stress inschizophrenia. Biochem. Biophys. Res. Commun., 479: 447-452, 2016.

9.

D. Abe, M. Arai, and M. Itokawa. Music-evoked emotions in schizophrenia.

Schizophr Res., doi.org/10.1016/j.schres.2016.12.013 published on line 2016.
10. A. Tarutani, G. Suzuki, A. Shimozawa, T. Nonaka, H. Akiyama, S.-I. Hisanaga, and
M. Hasegawa. The Effect of Fragmented Pathogenic ǂ-Synuclein Seeds on
Prion-like Propagation. J. Biol. Chem., 291: 18675-18688, 2016.
11. T. Oikawa, T. Nonaka, M. Terada, A. Tamaoka, S.-I. Hisanaga, and M. Hasegawa.
ǂ-Synuclein Fibrils Exhibit Gain of Toxic Function, Promoting Tau Aggregation andInhibiting Microtubule Assembly. J. Biol. Chem., 291: 15046-15056, 2016.
12. F. Kametani, T. Obi, T. Shishido, H. Akatsu, S. Murayama, Y. Saito, M. Yoshida,
and M. Hasegawa. Mass spectrometric analysis of accumulated TDP-43 in
amyotrophic lateral sclerosis brains. Sci. Rep., 6: 23281, 2016.
13. S. Shimonaka, T. Nonaka, G. Suzuki, S.-I. Hisanaga, and M. Hasegawa M.
Templated aggregation of TDP-43 by seeding with TDP-43 peptide fibrils. J. Biol.

Chem. 291: 8896-8907, 2016.
14. Y. Tanaka, T. Nonaka, G. Suzuki, F. Kametani, and M. Hasegawa. Gain-of-function
profilin 1 mutations linked to familial amyotrophic lateral sclerosis cause
seed-dependent intracellular TDP-43 aggregation. Hum. Mol. Genet., 25:
1420-1433, 2016.
15. T. Nonaka, G. Suzuki, Y. Tanaka, F. Kametani, S. Hirai, H. Okado, T. Miyashita, M.

― 367 ―

Systemic Organ Regulation

Saitoe, H. Akiyama, H. Masai, and M. Hasegawa. Phosphorylation of TAR
DNA-binding Protein of 43 kDa (TDP-43) by Truncated Casein Kinase 1ǅ Triggers
Mislocalization and Accumulation of TDP-43. J. Biol. Chem. 291: 5473-5483, 2016.
16. S. Taniguchi�Watanabe, T. Arai, F. Kametani, T. Nonaka, M. Masuda�Suzukake,
A. Tarutani, S. Murayama, Y. Saito, K. Arima, M. Yoshida. H. Akiyama, A.
Robinson, D. Mann, T. Iwatsubo, and M. Hasegawa. Biochemical classification oftauopathies by immunoblot, protein sequence and mass spectrometric analyses ofsarkosyl-insoluble and trypsin-resistant tau. Acta Neuropathol. 131: 267-280, 2016.

[Review Articles]
1.

R. Saiga, R. Mizutani, C. Inomoto, S. Takekoshi, N. Nakamura, A. Tsuboi, M.
Osawa, M. Arai, K. Oshima, M. Itokawa, K. Uesugi, A. Takeuchi, Y. Terada, and Y.
Suzuki. Three-dimensional structure of brain tissue at submicrometer resolution.

AIP Conference Proceedings., 1696, 2016.
2.

M. Hasegawa, T. Nonaka, and M. Masuda-Suzukake. Prion-like mechanisms andpotential therapeutic targets in neurodegenerative disorders. Pharmacol Ther, pii:
S0163-7258(16) 30236-4, 2016.

3.

M. Hasegawa. Molecular Mechanisms in the Pathogenesis of Alzheimer's disease
and Tauopathies-Prion-Like Seeded Aggregation and Phosphorylation.

Biomolecules, 6, 2016.
4.

Y. Tanaka, and M. Hasegawa. Profilin 1 mutants form aggregates that induce
accumulation of prion-like TDP-43. Prion 10: 283-9, 2016.

[Books]1.

M. Hasegawa. Molecular Biology of Dementia with Lewy Bodies, Dementia with

Lewy Bodies, Chapter 4: P41-58, Springer, Edited by Kenji Kosaka.
2.

M. Hasegawa, T. Nonaka, and M. Masuda-Suzukake. ǂ-Synuclein: Experimental
pathology. Cold Spring Harb. Perspect. Med. 1; 6(9), 2016.

[Conference Activities & Talks]
1.

K. Kitajima, K. Kitajima and T. Hara. In vitro differentiation of pluripotent stem
cells to hematopoietic stem cell-like cells (Oral presentation). 14th IGAKUKENInternational Symposium-IGAKUKEN Summit for Japan and Korea Science
Leaders 2016, 2016.6.30-7.1, Kamikitazawa.

2.

T. Hara, K. Tanegashima, R. Takahashi, H. Nuriya, N. Naruse, K. Tsuji, A.
Shigenaga, and A. Otaka. A Novel Function of a CXC-type Chemokine CXCL14 as― 368 ―

Systemic Organ Regulationa Specific Carrier of CpG DNA into Dendritic Cells for Activating Toll-like Receptor
9-mediated Adaptive Immunity. 58th American Society of Hematology Annual
Meeting and Exposition, 2016.12.3-6, San Diego.
3.

M. Hasegawa. Molecular mechanisms of TDP-43 aggregation. Brain Protein Agingand Dementia Control International Workshop, 2016.9.9, Nagoya.

4.

H. Okado. Regulation of brain development by transcription repressor RP58 12th
IGAKUKEN International Symposium IGAKUKEN Summit for Japan and Korea
Science Leaders 2016, 2016.7.29, Tokyo.

― 369 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

Clinical Anatomy

Professor Keiichi AKITAAssociate Professor Akimoto NIMURA(Exercise machinery and tools ability morphology lecture)
Junior Associate Professor Kumiko YAMAGUCHI(Department of Professional Development in Health Science)
Assistant Professor Masayo HARADA, Hisayo NASU
Takashi MIYAMOTO(Exercise machinery and tools ability morphology lecture)
Research Technician Kiyomi SAIKAWA, Masahiro TSUTSUMI
Parttime lecturer Kenji IBUKURO, Itsuko OKUDA,
Sachiyuki TSUKADA
Graduate Student Hitomi FUJISHIRO(∼March),Tatsuya TAMAKI(∼March),Yasuo NAKAJIMA,
Kazuhito SEKIZAWA,Keiko OKUMURA, Ryuhei OKADA, Eichirou KAGAWA,
Motoki TANAKA, Kotaro EGUCHI, Nobuaki KAWAI, Saya HORIUCHI,
Pawaree NONTHASAEN,Natnicha KAMPAN,Kentaro AMAHA,
Yusuke UEDA,Yasunori TATARA, Shota HOSHIKA, Phichaya BARAMEE,
Atsuhiko OCHI(April∼),Souichi HATTORI(April∼),Kou Miwa(April∼),
Satoru MURO(April∼),Suriyut JANARUK(April∼)
Research Student Hirokazu SAKAMOTO,Mamiko SUZUKI,Masataka NAKAZAWA(1)OutlineDepartment of Clinical Anatomy supports clinical medicine through formulation of human anatomical anddevelopmental biological bases of diagnoses and surgical procedures. We handle the whole body in humananatomical researches. We think it is classic but important to represent human morphology for exactly what
they are based on meticulous observations of human body structures regardless of diagnostic technics andsurgical procedures. Our researches are aimed to share languages among all clinicians based on clinical anatomy
by describing the results of observations in an accessible way for clinicians. In addition, we perform analyses
using experimental embryological approaches and developmental biological approaches, because we think it isimportant to consider how human structures are constructed.
(2)

Research

1) Clinical anatomic study of the shoulder joint and rotator cuﬀ.
2) Clinical anatomic study of the anal region for the rectoanal surgery.
3) Cadaveric study of the female pelvis for the gynecologic oncology and colposcopy.
4) Analyses of the lamination in the masticatory muscles with special reference of nerve supply.
5) Embryological study of the diﬀerentiation of cloaca and surrounding muscles.

(3)

Education

Clinical anatomy is generally considered as the practical application of anatomical knowledge to diagnosis andtreatment, however we think that this course is a part of pure anatomical science based on the ﬁndings of themorphological observations of the human bodies. Main objective of Clinical anatomy in the graduate course is to― 370 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

make detailed anatomical data to answer the questions developed from clinical ﬁelds especially by surgeons andradiologists. We collaborate with many clinicians: ENT, orthopedics, gynecology, thoracic surgery, radiology
and so on, and our projects have been broad areas. Students are expected to get ﬁne dissection techniques ofhuman bodies and also learn techniques of histology and embryological experiments. By using these techniques,
we study the spatial relationships of organs, vessels nerves, and also try to examine their developmental processes
in various projects.
(4)

Lectures & Courses

Theories and hypotheses of morphogenesis derived from descriptive anatomy and descriptive biology have been
conﬁrmed and modiﬁed by experimental biology. Furthermore, progresses of developmental biology identiﬁed
molecules and signaling pathways involved in the morphogenesis. Progresses in the developmental biology alsoveriﬁed morphological hypotheses, and added revisions to the morphological models. The postulates of themorphological models which are currently investigated were built and completed by Anatomy. However, we
ﬁnd Anatomy is still not completed and has many obscure issues through careful dissection of human body.
It might be thought that everything was done and there could be no new ﬁnding in the human anatomicalﬁeld anymore because the anatomy employs the classic procedures such as the gross anatomy. However, there
are still a lot of unclear anatomical topics, because they had not been focused and not investigated with theirclinical signiﬁcances.
(5)

Publications[Original Articles]
1. Tomomi Sakaguchi-Kuma, Nao Hayashi, Hitomi Fujishiro, Kumiko Yamaguchi, Kazuo Shimazaki, Takashi
Ono, Keiichi Akita. An anatomic study of the attachments on the condylar process of the mandible: muscle
bundles from the temporalis Surg Radiol Anat. 2016.05; 38(4); 461-467
2. Yuichiro Tsukada, Masaaki Ito, Kentaro Watanabe, Kumiko Yamaguchi, Motohiro Kojima, Ryuichi
Hayashi, Keiichi Akita, Norio Saito. Topographic Anatomy of the Anal Sphincter Complex and Levator Ani Muscle as It Relates to Intersphincteric Resection for Very Low Rectal Disease. Dis Colon
Rectum. 2016.05; 59(5); 426-433
3. Haruhiko Shimura, Akimoto Nimura, Hisayo Nasu, Hitomi Fujishiro, Junya Imatani, Atsushi Okawa,Keiichi Akita. Joint capsule attachment to the coronoid process of the ulna: an anatomic study with
implications regarding the type 1 fractures of the coronoid process of the O’Driscoll classiﬁcation. J
Shoulder Elbow Surg. 2016.09; 25(9); 1517-1522
4. Masataka Nakazawa, Akimoto Nimura, Tomoyuki Mochizuki, Masahiro Koizumi, Tatsuo Sato, Keiichi
Akita. The Orientation and Variation of the Acromioclavicular Ligament: An Anatomic Study. Am J
Sports Med. 2016.10; 44(10); 2690-2695
5. Kenji Ibukuro, Takaya Takeguchi, Hozumi Fukuda, Shoko Abe, Kimiko Tobe. Spatial anatomy of theround ligament, gallbladder, and intrahepatic vessels in patients with right-sided round ligament of theliver Surg Radiol Anat. 2016.11; 38(9); 1061-1067[Books etc]
1. Akimoto Nimura, Hisayo Nasu, Tomoyuki Mochizuki, Keiichi Akita. Advances in Shoulder Surgery.
Springer, 2016 (ISBN : 978-4-431-55986-3)
2. Keiichi Akita, Akimoto Nimura. Bergman’s Comprehensive Encyclopedia of Human Anatomic Variation.
Wiley, 2016 (ISBN : 978-1-1184-30354)[Conference Activities & Talks]
1. Yusuke Ueda, Hiroyuki Sugaya, Norimasa Takahashi, Keisuke Matsuki, Morihito Tokai, Kazutomo Onishi,
Shota Hoshika. Diﬀerence in Site of PASTA Lesion between Shoulders with Recurrent Anterior Instability
and Throwing Injuries. 2016 Annual Meeting 2016.03.02 Florida― 371 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

2. Mari Uomizu, Hideya Yoshimura, Akimoto Nimura, Takashi Miyamoto, Tomoyuki Mochizuki. Return toSports Activity after Arthroscopic Rotator Cuﬀ Repair on the Middle-aged and Senior Patients. The 13th
International Congress of Shoulder and Elbow Surgery 2016.05.18 Korea
3. Daisuke Ban, Keiichi Akita, Kumiko Yamaguchi, Hiroki Ueda, Minoru Tanabe. ANATOMICAL STUDY
OF THE UNCUS OF THE PANCREAS FOR THE SURGERY IN PANCREATODUODENECTOMY.
50th ANNUAL MEETING OF THE PANCREAS CLUB 2016.05.20 San Diego
4. Tatsuo Sato, Masashi Ito, Hirokazu Sakamoto. Two Major Lymphatic Pathways from the Gallbladder tothe Para-aortic Lymph Nodes(DVD). 33rd Annual Meeting American Association of Clinical Anatomists
2016.06.13 California
5. Keiichi Akita, Satoru Muro, Kumiko Yamaguchi, Hisayo Nasu, Phichaya Baramee, Janyaruk Suriyut.
Mutual Relationships among Perineal Muscles Based on Anatomic and Histologic Findings. 33rd Annual
Meeting American Association of Clinical Anatomists 2016.06.15 California
6. Itsuko Okuda, Keiichi Akita, Yasuo Nakajima. Magnetic Resonance-Thoracic and Ductography:Thoracic
Duct Variations and Contribution for the Thoracic Surgery. 33rd Annual Meeting American Associationof Clinical Anatomists 2016.06.15 California
7. Ryuhei Okada, Taro Sugimoto, Takuro Sumi, Taro Fujikawa, Yusuke Kiyokawa, Fuminori Nomura, Akihisa Tasaki, Tomoaki Asamori, Seiji Kishimoto, Takahiro Asakage. Chemotherapy and followed endoscopic laryngo-pharyngeal surgery (ELPS) is useful for recurrence of pharyngeal carcinoma: a case report.
AHNS 9TH International Conference on Head and Neck Cancer 2016.07.16 Washington
8. Keiichi Akita. Recent progresses of Clinical Anatomy of Shoulder joint with special reference to Rotator
cuﬀ muscles. Asian Association of Clinical anatomy 2016.08.06 China
9. Keiichi Akita. Research Application in Surgical Anatomy. Research Month for Surgical Resident Course
2016.08.25 Thailand
10. Satoru Muro, Phichaya Baramee, Janyaruk Suriyut, Hisayo Nasu, Masayo Harada, Kumiko Yamaguchi,
Keiichi Akita. Structural analysis of the anterior and posterior regions of the external anal sphincter; thespatial relationship with the bulbospongiosus and the coccyx. 8th ISCAA, 2016.08.31 Hungary
11. Kumiko Yamaguchi, Satoru Muro, Keiichi Akita. Anatomy of Treitz’s muscle of anal canal. 8th ISCAA
2016.08.31 Hungary
12. Natnicha Kampan, Itsuko Okuda, Hisayo Nasu, Kumiko Yamaguchi, Keiichi Akita. The Transitional
Muscle of Eyes and Its Complex Relations with Neighboring Muscles. The 8th International Symposiumof Clinical and Applied Anatomy; ISCAA 2016 2016.08.31 Hungary
13. Pawaree Nonthasaen, Hisayo Nasu, Eiichirou Kagawa, Akimoto Nimura, Keiichi Akita. The morphology
study of the extraforaminal ligaments in cervical and thoracic levels. 8th ISCAA 2016.08.31 Hungary
14. Keiko Okumura. Female sexual wellness; application of products. World meeting on sexual medicine
2016.09.22 China
15. Keiichi Akita. Trends in Anatomy Teaching for Clinical Education. International symposium of Medical
Education 2016.10.08 Taiwan
16. Pawaree Nonthasaen, Hisayo Nasu, Eiichirou Kagawa, Akimoto Nimura and Keiichi Akita. Changesof morphological features of the extraforaminal ligaments of the spinal nerve roots in cervico-thoracic
transitional zone. The 4th International Anatomical Sciences and Cell Biology Conference; IASCBC 20162016.12.04 Hong Kong
17. Natnicha Kampan, Itsuko Okuda, Hisayo Nasu, Kumiko Yamaguchi, Keiichi Akita. The Transition ofComplex Periorbital Muscle Bundles with Their Adjacent Muscles. The 4th International Anatomical
Sciences and Cell Biology Conference; IASCBC 2016 2016.12.06 HongKong
18. Keiichi Akita. Recent progresses of Clinical Anatomy of Shoulder joint with special reference to Rotator
cuﬀ muscles. Seminar in Department of Anatomy, Semmelweis University, Faculty of Medicine 2016.12.13
Poland― 372 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

Systems BioMedicine

Professor Hiroshi ASAHARA
Junior Associate Professor
Masahiro SHINOHARA
Project Junior Associate Professor
Masaki MORI
Tenure truck Assistant Professor
Yoshiaki ITOAssistant Professor
Satoshi YAMASHITA
Project Assistant Professor
Tomoki CHIBA, Takahide MATSUSHIMA
Project Researcher
Yoko TANAKA
Graduate Students
Takeshi SAITO, Kensuke KATAOKA, Shoya KITADA, Soichi FURUKAWA,
Naoki KODA, Hidetsugu SUZUKI, Takashi NAKASUJI, Hiroto YAMAMOTO,
Takahiro MITSUMURA, Ryo NAKAMICHI, Tomohiro KAYAMA, Masashi NAITO,
Yusuke MOCHIZUKI, Rin OKUMURA(1)

Research

We revealed that A to I RNA editing altered target genes of microRNAs using a reporter library.
The function and regulatory mechanisms of eIF2 gamma will be examined.
Search for novel regulator of microRNA by high throughput screening.
Established novel strategies for the osteoporosis by targeting molecules critical for the bone homeostasis.
Revealed the molecular mechanism by which osteocytes regulate bone homeostasis.
Analyzed the bone phenotype of spaceﬂight mice.
Analyzed molecular mechanisms by which the bone tissue regulates the energy metabolism.
The Screening of novel Damage-associated molecular patterns proteinsProtein localization analysis by High-throughput microscope system
MiRNA which regulates cartilage homeostasis was identiﬁed.
We developed screening system for miRNA target genes using reporter vector library.
MicroRNA KO mice were generated using CRISPR/Cas9 system, and its function in skeletal pattern formation
was analyzed.
Molecular mechanisms and in vivo roles of RNA-binding proteins and long non-coding RNAs in the context ofinﬂammatory response(2)

Education

Under Graduate:
Conducting Molecular Genetics , which is a series of lectures to understand the gene expression machineryand human genetics and their application to current medicine and biology. Under graduate students can join
the lab works to learn the skills for molecular biology and pathology.
Graduate School:
Organizing Development and Regeneration lecture series to understand the basis for regenerative medicine
and reproduction at the level of molecular genetics.
Students can join the Lab to perform researches using various experimental techniques, such as microarray, cellbased high throughput screening etc. Using these techniques, core molecular network for tissue development― 373 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced

and inﬂammatory diseases will be examined, which forms the basis of systems biomedicine.

(3)

Publications[Original Articles]
1. Masamichi Doiguchi, Takeya Nakagawa, Yuko Imamura, Mitsuhiro Yoneda, Miki Higashi, Kazuishi Kubota, Satoshi Yamashita, Hiroshi Asahara, Midori Iida, Satoshi Fujii, Tsuyoshi Ikura, Ziying Liu, Tulip
Nandu, W Lee Kraus, Hitoshi Ueda, Takashi Ito. SMARCAD1 is an ATP-dependent stimulator of nucleosomal H2A acetylation via CBP, resulting in transcriptional regulation. Sci Rep. 2016; 6; 20179
2. Shimbo M, Kudo T, Hamada M, Jeon H, Imamura Y, Asano K, Okada R, Tsunakawa Y, Mizuno S, Yagami
KI, Ishikawa C, Li H, Shiga T, Ishida J, Hamada J, Murata K, Ishimaru T, Hashimoto M, Fukamizu A,
Yamane M, Ikawa M, Morita H, Shinohara M, Asahara H, Akiyama T, Akiyama N, Sasanuma H, Yoshida
N, Zhou R, Wang YY, Ito T, Kokubu Y, Noguchi TK, Ishimine H, Kurisaki A, Shiba D, Mizuno H,
Shirakawa M, Ito N, Takeda S, Takahashi S.. Ground-based assessment of JAXA mouse habitat cage unit
by mouse phenotypic studies. Experimental Animals. 2016;
3. Kayama T, Mori M, Ito Y, Matsushima T, Nakamichi R, Suzuki H, Ichinose S, Saito M, Marumo K, Asahara H.. Gtf2ird1-dependent Mohawk (Mkx) expression regulates mechanosensing properties of tendon.
Mol Cell Biol. 2016.02;
4. Doiguchi M, Nakagawa T, Imamura Y, Yoneda M, Higashi M, Kubota K, Yamashita S, Asahara H, Iida M,
Fujii S, Ikura T, Liu Z, Nandu T, Kraus WL, Ueda H, Ito T. SMARCAD1 is an ATP-dependent stimulatorof nucleosomal H2A acetylation via CBP, resulting in transcriptional regulation Sci Rep. 2016.02;
5. Tomohiro Kayama, Masaki Mori, Yoshiaki Ito, Takahide Matsushima, Ryo Nakamichi, Hidetsugu Suzuki,
Shizuko Ichinose, Mitsuru Saito, Keishi Marumo, Hiroshi Asahara. Gtf2ird1-dependent Mohawk (Mkx)expression regulates mechanosensing properties of tendon. Mol. Cell. Biol.. 2016.02;
6. Otero M, Peng H, El Hachem K, Culley KL, Wondimu EB, Quinn J, Asahara H, Tsuchimochi K, Hashimoto
K, Goldring MB.. ELF3 modulates type II collagen gene (COL2A1) transcription in chondrocytes by
Inhibiting SOX9-CBP/p300-driven histone acetyltransferase activity. Connect Tissue Res.. 2016.06;
7. Suzuki H, Ito Y, Shinohara M, Yamashita S, Ichinose S, Kishida A, Oyaizu T, Kayama T, Nakamichi
R, Koda N, Yagishita K, Lotz MK, Okawa A, Asahara H.. Gene targeting of the transcription factor
Mohawk in rats causes heterotopic ossiﬁcation of Achilles tendon via failed tenogenesis. Proceedings of
the National Academy of Sciences of the United States of America. 2016.07; 113(28); 7840-7845
8. Naito M, Mori M, Inagawa M, Miyata K, Hashimoto N, Tanaka S, Asahara H.. Dnmt3a Regulates
Proliferation of Muscle Satellite Cells via p57Kip2. PLoS Genetics. . 2016.07; 12(7); e1006167
9. Ryo Nakamichi, Yoshiaki Ito, Masafumi Inui, Naoko Onizuka, Tomohiro Kayama, Kensuke Kataoka,
Hidetsugu Suzuki, Masaki Mori, Masayo Inagawa, Shizuko Ichinose, Martin K Lotz, Daisuke Sakai, Koichi
Masuda, Toshifumi Ozaki, Hiroshi Asahara. Mohawk promotes the maintenance and regeneration of theouter annulus ﬁbrosus of intervertebral discs. Nat Commun. 2016.08; 7; 12503
10. Asahara H*.. Current Status and Strategy of microRNA Research for Cartilage Development and Osteoarthritis Pathogenesis. J Bone Metab. . 2016.08; 23(3); 121-127
11. Saito T, Hara S, Tamano M, Asahara H, Takada S.. Deletion of conserved sequences in IG-DMR at
Dlk1-Gtl2 locus suggests their involvement in expression of paternally expressed genes in mice. J Reprod
Dev.. 2016.12;
12. Hironori Nakagami, Mariana Kiomy Osako, Yoichi Takami, Rie Hanayama, Hiroshi Koriyama, Masaki
Mori, Hiroki Hayashi, Hideo Shimizu, Ryuichi Morishita. Diﬀerential response of vascular hepatocyte
growth factor concentration and lipid accumulation between telmisartan and losartan in ApoE-deﬁcientmice. Mol Med Rep. 1(5); 657-661
13. Suzuki H, Ito Y, Shinohara M, Yamashita S, Ichinose S, Kishida A, Oyaizu T, Kayama T, Nakamichi R,
Koda N, Yagishita K, Lotz MK, Okawa A, Asahara H. Gene targeting of the transcription factor Mohawk
in rats causes heterotropic ossiﬁcation of Achilles tendon via failed tenogenesis. Proc Natl Acad Sci USA.
113; 7840-7845― 374 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

14. Koda N, Sato T, Shinohara M, Ichinose S, Ito Y, Nakamichi R, Kayama T, Kataoka K, Suzuki H,
Moriyama K, Asahara H. Mohawk transcription factor regulates homeostasis of the periodontal ligament.
Development. 144; 313-320
15. Ohmae S, Noma N, Toyomoto M, Shinohara M, Takeiri M, Fuji H, Takemoto K, Iwaisako K, Fujita T,
Takeda N, Kawatani M, Aoyama M, Hagiwara M, Ishihama Y, Asagiri M. Actin-binding protein coronin
1A controls osteoclastic bone resorption by regulating lysosomal secretion of cathepsin K Sci Rep. inpress;[Books etc]
1. Y. Ito, H. Asahara. non-coding miRNA. 2016.06
2. Y. Ito, S.Mokuda, K.Miyata, T. Matsushima, H. Asahara. Chronic Inﬂammation. Springer, 2016.11
3. Ito Y, Mokuda S, Miyata K, Matsushima T, Asahara H*. . Chronic Inﬂammation (Chapter 6). Springer,
2016.11[Misc]
1. T. Chiba, Y. Ito, H. Asahara.. Post-Transcriptional Gene Regulation by MicroRNA and RNA-Binding
Protein Biomedical Sciences. 2016;
2. Gene targeting of the transcription factor Mohawk in rats causes heterotopic ossiﬁcation of Achilles tendon
via failed tenogenesis 2016.12;[Conference Activities & Talks]
1. Koda N, Shinohara M, et al.. Roles of transcription factor Mohawk in periodontal ligament. International
Bone and Mineral Society Herbert Fleisch Workshop 2016 2016.02
2. Elucidating The Transcriptional Network of Mechanosensitive Tendon Master Gene Mohawk (Mkx).
2016.03
3. Tomohiro Kayama,Masaki Mori,Yoshiaki Ito,Ryo Nakamichi,Takahide Matsushima,Shizuko Ichinose,Mitsuru
saito,Keishi Marumo,Hiroshi Asahara. Elucidating The Transcriptional Network Of Mechanosensitive
Tendon Master Gene Mohawk(Mkx). Orthopaedic Research Society Annual meeting 2016 Orlando 2016.03.06
Florida4. Hidetsugu Suzuki. Mohawk Knock-Out Rats Revealed Early Heterotopic Ossiﬁvation of Achilles Tendon
Through Failed Tenogenesis. Orthopaedic Research Society Annual meeting 2016 Orlando 2016.03.07
Florida5. Yoko Tanaka-Watanabe, et al.. MicroRNA-381 ameliorates arthritis by controlling functions of ﬁbroblastlike synoviocytes, which are aﬀected by A to I RNA editing.. Annual European Congress of Rhumatology
2016 2016.06.08 London, UK
6. Hiroshi Asahara. Gene targeting of the transcription factor Mohawk in rats causes heterotopic ossiﬁcationof Achilles tendon via failed tenogenesis. Stem Cell, Growth Factor Signaling and Bone Biology 2016.07.25
Hangzhou
7. Yoko Tanaka, et al.. Regulation of arthritis by microRNA-381. 2016.10.14 Tokyo, Japan
8. Kensuke Kataoka、Ysoshiaki ITO,Tomoki CHIBA,Ryo NAKAMICHI,HiroshiASAHARA. Tendon Regeneration and Development by Transcription Factor Mkx. Visual-JW 2016 2016.10.17
9. Takahide MATSUSHIMA、Naoki GOSHIMA,Hiroshi ASAHARA. The High-Throughput Microscopy Screening System with Genome-Wide cDNA Expression Libraries. Visual-JW 2016 2016.10.17
10. Yoko Tanaka-Watanabe, et al.. MicroRNA-381 mimic resulted in a therapeutic eﬀect on arthritis bycontrolling several functions of ﬁbroblast-like synoviocytes.. Cold Spring Harbor Asia conference on Bone& Cartilage: From Development to Human Diseases 2016.10.25 Suzhou, China
11. Yoko Tanaka-Watanabe, . A to I RNA editing of microRNA-381 dysregulates proliferation of ﬁbroblastlike synoviocytes. Cell Symposia: Functional RNAs. Cell Symposia: Functional RNAs 2016.11.07― 375 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced

12. Yoko Tanaka, et al.. Function of microRNA-381 in arthritis. 2016.11.30 yokohama, Japan
13. Koda N, Shinohara M, et al.. Roles of transcription factor Mohawk in periodontal ligament. International
Association for Dental Research 2016 2016.12
14. Y.Ito, A. Inoue, T. Seers, Y. Hato, A. Igarashi, T. Toyama, H. Asahara. Identiﬁcation of targets of
microRNA-34a using a reporter library system. The 39th Annual Meeting of the Molecular Biology
Society of Japan 2016.12.02[Awards & Honors]
1. Pediatric Academic Societies (PAS) and Asian Society for Pediatric Research (ASPR) Joint Meeting 2014,
Travel Award North America― 376 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

Comprehensive Pathology

Professor Masanobu KITAGAWA
Junior assocoate Professor Morito KURATA
Assistant Professor Kouhei YAMAMOTO, Iichiro ONISHI
Laboratory Technician Miori INOUE
Technical Assistant Sachiko ISHIBASHI, Masumi IKEDA,
Graduate Students Xiao Hai JIN, Yuko KINOWAKI,
Atsushi KIHARA, GULINISHA Aihemaiti
Masae Yanai, Mariko Muto,
Ryoko KATO, Naoko OZAWA
Norikazu MIYAMOTO, Masafumi INOUE,
Keiichi KINOWAKI, Naoko Tsuyama,
Yusuke FURUKAWA, Risa Hou,
Sumito Shingaki, Masanori Matsuda,
Tan Wang, Akiko YAMAMOTO,
Miyaka Umemori, Momoko Yamada,
Takuya Maeda, Miu katsuyama,
Tatsunori Mineo, Vilayvong Sulideyh,
Luangxay Thitsamay,
Abudushalamu Muyashaer,(1)

OutlineMain objective of comprehensive pathology in the graduate course is to acquire the technique of clinical andbasic pathology. This course provides students opportunity to study clinical pathology (for example, histologicaland cytological diagnosis, autopsy, clinico-pathologic conference) and also basic pathology (molecular pathology
and molecular biology).
(2)

Research

1) Clinico-pathological study by morphological ﬁndings, immunohistochemistry, and electron microscope, etc.
2) Molecular analysis of leukomogenesis induced by Friend leukemia virus (FLV)
3) Enhancement of apoptosis by virus-derived protein and development of apoptosis-induction cancer therapy4) Molecular pathology of the myelodysplastic syndromes (MDS)
5) Clariﬁcation of drug resistance mechanism for hematopoietic malignancies
6) Comprehensive research for aging focus on the decreased immune competence7) Molecular biology of the cancer progression and metastasis(3)

Education

Main objective of comprehensive pathology in the graduate course is to acquire the techniques of clinical andbasic pathology. This course provides students opportunities to study clinical pathology (for example, histological and cytological diagnosis, autopsy, clinico-pathologic conference) and also basic pathology (molecularpathology and molecular biology).

― 377 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced(4)

Publications[Original Articles]
1. Yusuke F, Kentaro T, Katsuhide I, Maky IO, Kenichi A, Satoshi K, Masanobu K, Jun K. Learning andMemory Deﬁcits in Male Adult Mice Treated with a Benzodiazepine Sleep-Inducing Drug during theJuvenile Period Front. Neurosci.. 2016; 10; 3389
2. Susumu Kirimura, Morito Kurata, Yasunori Nakagawa, Iichiroh Onishi, Shiho Abe-Suzuki, Shinya Abe,
Kouhei Yamamoto, Masanobu Kitagawa. Role of microRNA-29b in myelodysplastic syndromes duringtransformation to overt leukaemia. Pathology. 2016.04; 48(3); 233-241
3. Kazuhito Suzuki, Kouhei Yamamoto, Yasuhiro Arakawa, Hisashi Yamada, Keisuke Aiba, Masanobu Kitagawa. Antimyeloma activity of bromodomain inhibitors on the human myeloma cell line U266 by downregulation of MYCL. Anticancer Drugs. 2016.09; 27(8); 756-765
4. Shunichiro Yasuda, Keisuke Tanaka, Ayako Ichikawa, Ken Watanabe, Emi Uchida, Masahide Yamamoto,
Kouhei Yamamoto, Daisuke Mizuchi, Osamu Miura, Tetsuya Fukuda. Aggressive TAFRO syndrome withreversible cardiomyopathy successfully treated with combination chemotherapy. Int. J. Hematol.. 2016.10;
104(4); 512-518
5. Sumito Shingaki, Yumiko Yoshiki, Kouhei Yamamoto, Yasunori Ota, Kenshi Suzuki. Coexistent adrenal
diﬀuse large B cell lymphoma in a patient with Waldenström’s macrogloblinemia/lymphoplasmacytic
lymphoma. Ann. Hematol.. 2016.10; 95(10); 1723-1724
6. Jin XH, Yamamoto K, Abe S, Onishi I, Kirimura S, Aihemaiti G, Kinowaki Y and Kitagawa M. Prognostic
Impact of Cancer Stem Cell-Like Phenotypes in Pancreatic Ductal Adenocarcinoma Journal of Clinicaland Experimental Pathology . 2016.10; 6(295);
7. Morito Kurata, Susan K Rathe, Natashay J Bailey, Natalie K Aumann, Justine M Jones, G Willemijn
Veldhuijzen, Branden S Moriarity, David A Largaespada. Using genome-wide CRISPR library screening
with library resistant DCK to ﬁnd new sources of Ara-C drug resistance in AML. Sci Rep. 2016.11; 6;
36199[Misc]
1. Kouhei Yamamoto, Yukako Miwa, Shiho Abe-Suzuki, Shinya Abe, Susumu Kirimura, Iichiroh Onishi,
Masanobu Kitagawa, Morito Kurata. Extramedullary hematopoiesis: Elucidating the function of thehematopoietic stem cell niche (Review). Mol Med Rep. 2016.01; 13(1); 587-591― 378 ―

Advanced
Advanced Therapeutic
Therapeutic Sciences
Sciences

Molecular Oncology

Professor: Shinji TANAKA
Associate Professor: Yoshimitsu AKIYAMA
Associate Professor: Hiroshi FUKAMACHI
Assistant Professor: Shu SHIMADA
Laboratory Technician: Hiromi NAGASAKI
Graduate Student: Ayuna SAKAMOTO
Graduate Student: Taketo NISHIKAWAJI(1) OutlineTo understand the molecular mechanisms underlying carcinogenesis BOE malignant  progression for
clinicalapplica-tion of cancer prevention, diagnosis and treatment.
(2) Research Molecular analysis of refractory malignancies including liver, pancreatic and scirrhous gastric cancers
 Development of molecularly targeted therapy for refractory malignancies
 Cancer epigenetics/epigenomics and clinical application in refractory malignancies
 Research of cancer stem cells and targFted therapy
 Development of regenerative medicine using stem cell research(3)

Education

Hygiene is our charge. The undergraduate curriculum of hygiene includes lectures, and laboratory studies.
Topics of lectures consist of environmental pollution and human health, world-wide environmental problems,
carcinogen and occupational cancer, smoking-related diseases, infectious diseases including AIDS and hepatitis,
food poisoning, anoxia and heat-related diseases.
(4)

Lectures & Courses

The graduate students pursue their own projects associated with one of researches being in progress in thedivision. Every student can learn the basic scientiﬁc techniques, such as genetic engineering, cell culture andbiochemical procedures. There are also many special lectures on cancer, gene, cell biology and biochemistry
for the graduate students. On weekly seminars, the students present their own research data and introduce
important papers from newly-arrived journals. Once the students get new ﬁndings, they are encouraged topresent them at the domestic or international meeting and write manuscripts.
(5)

Publications[Original Articles]
1. Ito H, Tanaka S, Akiyama Y, Shimada S, Adikrisna R, Matsumura S, Aihara A, Mitsunori Y, Ban D,
Ochiai T, Kudo A, Arii S, Yamaoka S, Tanabe M. Dominant Expression of DCLK1 in Human Pancreatic― 379 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced

Cancer Stem Cells Accelerates Tumor Invasion and Metastasis. PLoS ONE. 2016.01; 11(1); e0146564
2. Akiyama Y, Koda Y, Byeon SJ, Shimada S, Nishikawaji T, Sakamoto A, Chen Y, Kojima K, Kawano
T, Eishi Y, Deng D, Kim WH, Zhu WG, Yuasa Y, Tanaka S. Reduced expression of SET7/9, a histone
mono-methyltransferase, is associated with gastric cancer progression. Oncotarget. 2016.01; 7(4); 39663983
3. Katsuta E, Tanaka S, Mogushi K, Shimada S, Akiyama Y, Aihara A, Matsumura S, Mitsunori Y, Ban D,
Ochiai T, Kudo A, Fukamachi H, Tanaka H, Nakayama K, Arii S, Tanabe M. . CD73 as a therapeutictarget for pancreatic neuroendocrine tumor stem cells. International Journal of Oncology . 2016.02; 48;
657-669
4. Fukiko Kawai-Kitahata, Yasuhiro Asahina, Shinji Tanaka, Sei Kakinuma, Miyako Murakawa, Sayuri Nitta,
Takako Watanabe, Satoshi Otani, Miki Taniguchi, Fumio Goto, Hiroko Nagata, Shun Kaneko, Megumi
Tasaka-Fujita, Yuki Nishimura-Sakurai, Seishin Azuma, Yasuhiro Itsui, Mina Nakagawa, Minoru Tanabe,
Shinichi Takano, Mitsuharu Fukasawa, Minoru Sakamoto, Shinya Maekawa, Nobuyuki Enomoto, Mamoru
Watanabe. Comprehensive analyses of mutations and hepatitis B virus integration in hepatocellular
carcinoma with clinicopathological features. J. Gastroenterol. 2016.03; 51(5); 473-486
5. Lentjes MH, Niessen HE, Akiyama Y, de Bruïne AP, Melotte V, van Engeland M. The emerging role of
GATA transcription factors in development and disease. Expert Rev Mol Med. 2016.03; 18; e3
6. Furuyama T, Tanaka S, Shimada S, Akiyama Y, Matsumura S, Mitsunori Y, Aihara A, Ban D, Ochiai T,
Kudo A, Fukamachi H, Arii S, Kawaguchi Y, Tanabe M. Proteasome activity is required for the initiationof precancerous pancreatic lesions. Sci Rep. 2016.05; 6; 27044
7. Eriko Katsuta, Atsushi Kudo, Takumi Akashi, Yusuke Mitsunori, Satoshi Matsumura, Arihiro Aihara,
Daisuke Ban, Takanori Ochiai, Shinji Tanaka, Yoshinobu Eishi, Minoru Tanabe. Macroscopic morphology
for estimation of malignant potential in pancreatic neuroendocrine neoplasm. J. Cancer Res. Clin. Oncol..
2016.06; 142(6); 1299-1306
8. Akahoshi K, Tanaka S, Mogushi K, Shimada S, Matsumura S, Akiyama Y, Aihara A, Mitsunori Y, Ban
D, Ochiai T, Kudo A, AriiS, Tanabe M. Expression of connective tissue growth factor in the livers of
non-viral hepatocellular carcinoma patients with metabolic risk factors. J. Gastroenterol.. 2016.09; 51(9);910-922
9. Ojima F, Saito Y, Tsuchiya Y, Kayo D, Taniuchi S, Ogoshi M, Fukamachi H, Takeuchi S, Takahashi
S. Runx3 transcription factor regulates ovarian functions and ovulation in female mice J Reprod Dev.
2016.10; 62(5); 479-486
10. Nishikawaji T, Akiyama Y, Shimada S, Kojima K, Kawano T, Eishi Y, Yuasa Y, Tanaka S. Oncogenic rolesof the SETDB2 histone methyltransferase in gastric cancer. Oncotarget. 2016.10; 7(41); 67251-67265
11. Diaz-Lagares A, Crujeiras AB, Lopez-Serra P, Soler M, Setien F, Goyal A, Sandoval J, Hashimoto Y,
Martinez-Cardús A, Gomez A, Heyn H, Moutinho C, Espada J, Vidal A, Paúles M, Galán M, Sala N,
Akiyama Y, Martínez-Iniesta M, Farré L, Villanueva A, Gross M, Diederichs S, Guil S, Esteller M. Epigenetic inactivation of the p53-induced long noncoding RNA TP53 target 1 in human cancer. Proceedingsof the National Academy of Sciences of the United States of America. 2016.11; 113(47); E7535-E7544
12. Munakata K, Uemura M, Tanaka S, Kawai K, Kitahara T, Miyo M, Kano Y, Nishikawa S, Fukusumi T,
Takahashi Y, Hata T, Nishimura J, Takemasa I, Mizushima T, Ikenaga M, Kato T, Murata K, Carethers
JM, Yamamoto H, Doki Y, Mori M. Cancer stem-like properties in colorectal cancer cells with low proteasome activity Clinical Cancer Research. 2016.11; 22(21); 5277-5286
13. Watanabe Y, Honda S, Konishi A, Satoko Arakawa S, Murohashi M, Yamaguchi H, Torii S, Tanabe M,
Tanaka S, Warabi E, Shimizu S. Autophagy controls centrosome number by degrading Cep63. Nature
Communications. 2016.11; 21(7); 13508
14. Furuyama T, Kudo A, Matsumura S, Mitsunori Y, Aihara A, Ban D, Ochiai T, Tanaka S, Tanabe M. Preoperative direct bilirubin to prothrombin time ratio index to prevent liver failure after minor hepatectomy
J Hepatobiliary Pancreat Sci.. 2016.12; 23(12); 763-770― 380 ―

Advanced
Advanced Therapeutic
Therapeutic Sciences
Sciences

15. Oba A, Shimada S, Akiyama Y, Nishikawaji T, Mogushi K, Ito H, Matsumura S, Aihara A, Mitsunori
Y, Ban D, Ochiai T, Kudo A, Asahara H, Kaida A, Miura M, Tanabe M, Tanaka S . ARID2 modulates
DNA damage response in human hepatocellular carcinoma cells Journal of Hepatology, in press.
16. Ueda H, Ban D, Akahoshi K, Mitsunori Y, Matsumura S, Ochiai T, Kudo A, Tanaka S, Tanabe M.
Refractory long-term cholangitis after pancreaticoduodenectomy: a retrospective study World Journal ofSurgery, in press.
17. Oba A, Ban D, Kirimura S, Akahoshi K, Mitsunori Y, Matsumura S, Ochiai T, Kudo A, Tanaka S, Tanabe
M. . Clinical application of the biomarkers for the selection of adjuvant chemotherapy in pancreatic ductal
adenocarcinoma. J Hepatobiliary Pancreat Sci., in press.
18. Ohata Y, Akiyama Y, Shimada S, Mogushi K, Ito H, Matsumura S, Aihara A, Mitsunori Y, Ban D, Ochiai
T, Kudo A, Tanabe M, Tanaka S. . Acquired resistance with epigenetic alterations under long-term antiangiogenic therapy for hepatocellular carcinoma Molecular Cancer Therapeutics, in press.
[Books etc]
1. Tanaka S. Cancer stem cells as therapeutic targets. in Human Stem Cell Toxicity, ed. Sherley JL. (Royal
Society of Chemistry, London), 2016
2. Fukamachi H. Encyclopedia of Cancer, 4th ed. Springer, 2016.01[Misc]
1. Tanaka S.. Molecular pathogenesis and targeted therapy of pancreatic cancer. Annals of Surgical Oncology. 2016.02; Suppl (2); 197-205
2. Tanaka S. Molecular analysis for therapeutic targets of pancreatic cancer. in Molecular diagnosis andtargeting for thoracic and gastrointestinal malignancy, eds. Shimada Y, Yanaga K. (Springer), in press.
[Conference Activities & Talks]
1. Shimada S, Akiyama Y, Fukamachi H, Yuasa Y, Tanaka S. Identiﬁcation of selective inhibitors of diﬀusetype gastric cancer cells by screening annotated compounds. AACR Annual Meeting 2016 2016.04.18 New
Orleans, LA
2. Akiyama Y, Nishikawaji T, Shimada S, Yuasa Y, Tanaka S. Expression changes of the histone methyltransferase genes, SETDB2, in gastric cancers. 26th Seoul International Cancer Symposium 2016.06.17
Seoul, Korea
3. Fukamachi H, Nishikawaji T, Shimada S, Akiyama Y, Yuasa Y, Tsuchiya K, Tanaka S. Characteristics
of diﬀuse-type gastric cancer stem cells. 26th Seoul International Cancer Symposium 2016.06.17 Seoul,
Korea
4. Akiyama Y, Nishikawaji T, Shimada S, Sakamoto A, Deng D, Kim WH, Zhu WG, Yuasa Y, Tanaka S.
Reduced expression and its function of SET7/9, a histone methyltransferase gene, in gastric cancer. The
35th Sapporo International Cancer Symposium 2016.06.24 Sapporo
5. Tanaka S, Ito H, Furuyama T, Aihara A, Matsumura S, Mitsunori Y, Ban D, Ochiai T, Kudo A, Tanabe
M. Personalized medicine to target the cancer stemness of hepato-biliary-pancreatic malignancies (Symposium).. The 72th Annual Meeting of the Japanese Society of Gastroenterological Surgery 2016.07.15
Tokushima
6. Nishikawaji T, Akiyama Y, Shimada S, Yuasa Y, Tanaka S. SETDB2 contributes to gastric cancer progression by deregulating the expression of tumor suppressor genes. The 75th Annual Meeting of the JapaneseCancer Association 2016.10.06 Yokohama
7. Tanaka S, Shimada S, Akiyama Y. Epigenetic target of stemness for metastasis and therapeutic resistancein patients with pancreatic and liver cancers. The 75th Annual Meeting of the Japanese Cancer Association
2016.10.06 Yokohama― 381 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced

8. Fukamachi H, Nishikawaji T, Shimada S, Akiyama Y, Yuasa Y, Tanaka S. Identiﬁcation of MTDs thatsuppress the growth of PDX-derived duﬀuse-type gastric tumor-initiating cells. The 75th Annual Meetingof the Japanese Cancer Association 2016.10.06 Yokohama
9. Akiyama Y, Nishikawaji T, Shimada S, Sakamoto A, Yuasa Y, Tanaka S. Reduced expression of SET7/9,
a histone mono-methyltransferase, is associated with gastric cancer progression. The 75th Annual Meetingof the Japanese Cancer Association 2016.10.07 Yokohama
10. Shimada S, Akiyama Y, Fukamachi H, Yuasa Y, Tanaka S. Identiﬁcation of selective inhibitors of diﬀusetype gastric cancer cells by screening of annotated compounds. The 75th Annual Meeting of the JapaneseCancer Association 2016.10.07 Yokohama
11. Akiyama Y, Nishikawaji T, Shimada S, Deng D, Kim WH, Zhu WG, Yuasa Y, Tanaka S. Expression
changes of the histone H3 lysine methyltransferase genes and their function in gastric cancer. 5th International Cancer Epigenetics Meeting 2016.10.22 Beijing, China[Patents]
1. Dominant negative mutants of IRS-1 and uses there of (Tanaka S, Wands JR), Patent Number：UnitedStates Patent 6,528,479
2. Compositions and methods for detection and treatment of hepatocellular carcinoma (Tanaka S, MacDonald G), Application Number：US 61/811,360― 382 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

Surgical Pathology

Professor：Yoshinobu EISHI
Associate Professor：Takumi AKASHI,Masanobu KITAGAWA
Assistant Professor：Emiko SUGAWARA,Susumu KIRIMURA,
Shohei TOMII,Atsuko KONTA
Hospital Staﬀ Doctor：Keiko MIURA
Secretary：Ayako UENO
(1)

OutlineMissons of diagnostic pathology are summarized to following 4 items. 1) participation to the medical treatmentof the patients through anatomical diagnosis 2) assessment of medical treatment through autopsy examination 3)
training of diagnostic pathologists 4) development of diagnostic methods by anatomical, immunohistochemical,
microbiological and molecular technologies.
In cooperation with departments of human pathology and comprehensive pathology, department of surgicalpathology provides 1. diagnostic pathology services for the clinicians of the aﬃliated hospital 2. education of
medical students and post-graduate students through both lectures and medical practice 3. development of new
methods in diagnosticl pathology.

(2)

Research

1)Analysis of the pathophysiology of the disease, especially invasion mechanism of lung and gastrointestinal
cancers by molecular biological technology.
2)Identiﬁcation of epithelial neoplasms with chromosome translocation
3)Carcinogenesis of prostatic cancer in view of microbiology(3)

Education

Main object of surgical pathology in the course of graduate school is to provide medical students opportunity
to study pathophysiology and diagnosis of core diseases, both neoplastic and non-neoplastic, through biopsy,
surgical and autopsy cases. Another important mission is a training of pathology specialist in the post-graduate
school through diagnostic services of surgical pathology, cytopathology and autopsy.
(4)

Lectures & Courses

The initial purpose of this program is to acquire how to morphologically diagnose both neoplastic and nonneoplastic diseases. In addition, it is also very important to recognize the limitations and problems of morphological diagnosis and to learn the morphological and molecular methods which are necessary for the resolutionof the problems. The ultimate purpose is to develop a new diagnostic method which can resolve the problems
of morphological diagnosis.

― 383 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced(5)

Clinical Services & Other Works

In cooperation with departments of human pathology and comprehensive pathology, department of surgicalpathology provides autopsy services (38 case in a year), cytopathology services (9,957 cases in a year) andsurgical pathology (11,574 cases in a year) for the clinicians of the aﬃliated hospital. Diagnosis is mostly doneby the organ-subspecilized staﬀs. Clinico-pathological conferences have been held about two hundred times in
2014.
(6)

Clinical Performances

Department of diagnostic pathology participates in the medical treatment of the patients through anatomical
diagnosis. In the era of molecule-targeted therapy, specialized information has been requested in the ﬁeld of
pathological diagnosis. In order to appropriately respond to a latest request of clinicians, we practice pathological diagnosis in cooperation with departments of human pathology and comprehensive pathology with latest
techniques, such as immunohistochemistry, electron microscopy, and FISH.
(7)

Publications[Original Articles]
1. Kou Kayamori, Ken-Ichi Katsube, Kei Sakamoto, Yoshio Ohyama, Hideaki Hirai, Akane Yukimori, Yae
Ohata, Takumi Akashi, Masao Saitoh, Kiyoshi Harada, Hiroyuki Harada, Akira Yamaguchi. NOTCH3 Is
Induced in Cancer-Associated Fibroblasts and Promotes Angiogenesis in Oral Squamous Cell Carcinoma.
PLoS ONE. 2016; 11(4); e0154112
2. Teruko Nakamura, Asuka Furukawa, Keisuke Uchida, Tomohisa Ogawa, Tomoki Tamura, Daisuke Sakonishi, Yuriko Wada, Yoshimi Suzuki, Yuki Ishige, Junko Minami, Takumi Akashi, Yoshinobu Eishi. Autophagy Induced by Intracellular Infection of Propionibacterium acnes. PLoS ONE. 2016; 11(5); e0156298
3. Akiyama Y, Koda Y, Byeon SJ, Shimada S, Nishikawaji T, Sakamoto A, Chen Y, Kojima K, Kawano
T, Eishi Y, Deng D, Kim WH, Zhu WG, Yuasa Y, Tanaka S. Reduced expression of SET7/9, a histone
mono-methyltransferase, is associated with gastric cancer progression. Oncotarget. 2016.01; 7(4); 39663983
4. Okada T, Tanaka K, Kawachi H, Ito T,Nishikage T,Odagaki T,Zárate AJ, Kronberg U, López-Köstner
F,Karelovic S, Flores S, Estela R, Tsubaki M, Uetake H, Eishi Y, Kawano T. International collaboration
between japan and chile to improve detection rates in colorectal cancer screening. Cancer. 2016.01; 122(1);71-77
5. Atsuko Konta, Kouichi Ozaki, Yasuhiko Sakata, Atsushi Takahashi, Takashi Morizono, Shinichiro Suna,
Yoshihiro Onouchi, Tatsuhiko Tsunoda, Michiaki Kubo, Issei Komuro, Yoshinobu Eishi, Toshihiro Tanaka.
A functional SNP in FLT1 increases risk of coronary artery disease in a Japanese population. J. Hum.
Genet.. 2016.01;
6. Nobuo Sanjo,Satoko Kina,Yukiko Shishido-Hara,Yurie Nose,Satoru Ishibashi,Tetsuya Fukuda,Taketoshi
Maehatra,Yoshinobu Eishi,Hidehiro Mizusawa,Takanori Yokota. A Case of Progressive Multifocal Leukoencephalopathy with Balanced CD4/CD8 T-Cell Inﬁltration and Good Response to Meﬂoquine Treatment.
Internal Medicine. 2016.01; 55(12); 1631-1635
7. Yamada I, Hikishima K, Miyasaka N, Kato K, Kojima K, Kawano T, Ito E, Kobayashi D, Eishi Y, Okano
H. Gastric carcinoma: evaluation with diﬀusion-tensor MR imaging and tractography ex vivo. Magn
Reson Imaging. 2016.02; 34(2); 144-151
8. Tadahisa Numakura, Tsutomu Tamada, Masayuki Nara, Soshi Muramatsu, Koji Murakami, Toshiaki
Kikuchi, Makoto Kobayashi, Miho Muroi, Tatsuma Okazaki, Sho Takagi, Yoshinobu Eishi, Masakazu
Ichinose. Simultaneous development of sarcoidosis and cutaneous vasculitis in a patient with refractory
Crohn’s disease during inﬂiximab therapy. BMC Pulm Med. 2016.02; 16; 30
9. Ryohei Hayashi, Kiichiro Tsuchiya, Keita Fukushima, Nobukatsu Horita, Shuji Hibiya, Keisuke Kitagaki,
Mariko Negi, Eisaku Itoh, Takumi Akashi, Yoshinobu Eishi, Eriko Okada, Akihiro Araki, Kazuo Ohtsuka,
Shinji Fukuda, Hiroshi Ohno, Ryuichi Okamoto, Tetsuya Nakamura, Shinji Tanaka, Kazuaki Chayama,― 384 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

Mamoru Watanabe. Reduced Human α-defensin 6 in Noninﬂamed Jejunal Tissue of Patients with Crohn’s
Disease. Inﬂamm. Bowel Dis. 2016.05; 22(5); 1119-1128
10. Nariaki Kokuho, Yasuhiro Terasaki, Hirokazu Urushiyama, Mika Terasaki, Shinobu Kunugi, Taisuke
Morimoto, Arata Azuma, Jitsuo Usuda, Akihiko Gemma, Yoshinobu Eishi, Akira Shimizu. Pulmonary
mucosa-associated lymphoid tissue lymphoma associated with pulmonary sarcoidosis: a case report andliterature review. Hum. Pathol.. 2016.05; 51; 57-63
11. Masahiro Asakawa, Takanobu Yoshimoto, Mitsutane Ota, Mitsuyuki Numasawa, Yuriko Sasahara, Takato
Takeuchi, Yujiro Nakano, Norihiko Oohara, Masanori Murakami, Ryotaro Bouchi, Isao Minami, Kyoichiro Tsuchiya, Koshi Hashimoto, Hajime Izumiyama, Naoko Kawamura, Kazunori Kihara, Mariko
Negi, Takumi Akashi, Yoshinobu Eishi, Hironobu Sasano, Yoshihiro Ogawa. A Case of Cushing’s Syndrome with Multiple Adrenocortical Adenomas Composed of Compact Cells and Clear Cells. Endocr.
Pathol.. 2016.06; 27(2); 136-141
12. Sanae Shimamura, Naoto Yokogawa, Kengo Murata, Tetsuo Yamaguchi, Keisuke Uchida, Yoshinobu Eishi.
Saddle nose with sarcoidosis: "A great imitator" of relapsing polychondritis. Mod Rheumatol. 2016.06;
1-5
13. Eriko Katsuta, Atsushi Kudo, Takumi Akashi, Yusuke Mitsunori, Satoshi Matsumura, Arihiro Aihara,
Daisuke Ban, Takanori Ochiai, Shinji Tanaka, Yoshinobu Eishi, Minoru Tanabe. Macroscopic morphology
for estimation of malignant potential in pancreatic neuroendocrine neoplasm. J. Cancer Res. Clin. Oncol..
2016.06; 142(6); 1299-1306
14. Sahoko Chiba, Kimitake Tsuchiya, Takumi Akashi, Masahiro Ishizuka, Tsukasa Okamoto, Haruhiko Furusawa, Tomoya Tateishi, Mitsuhiro Kishino, Yasunari Miyazaki, Ukihide Tateishi, Tamiko Takemura,
Naohiko Inase. Chronic Hypersensitivity Pneumonitis With a Usual Interstitial Pneumonia-Like Pattern:
Correlation Between Histopathologic and Clinical Findings. Chest. 2016.06; 149(6); 1473-1481
15. Yamada I, Hikishima K, Miyasaka N, Kato K, Ito E, Kojima K, Kawano T, Kobayashi D, Eishi Y, Okano
H. q-Space MR imaging of gastric carcinoma ex vivo: correlation with histopathologic ﬁndings. Magn
Reson Med. 2016.08; 76(2); 602-612
16. Ryuichi Okamoto, Mariko Negi, Syohei Tomii, Yoshinobu Eishi, Mamoru Watanabe. Diagnosis andtreatment of microscopic colitis. Clin J Gastroenterol. 2016.08; 9(4); 169-174
17. Chihiro Takasaki, Masashi Kobayashi, Hironori Ishibashi, Takumi Akashi, Kenichi Okubo. Expressionof hypoxia-inducible factor-1 α aﬀects tumor proliferation and antiapoptosis in surgically resected lungcancer. Mol Clin Oncol. 2016.08; 5(2); 295-300
18. Nishikawaji T, Akiyama Y, Shimada S, Kojima K, Kawano T, Eishi Y, Yuasa Y, Tanaka S. Oncogenic rolesof the SETDB2 histone methyltransferase in gastric cancer. Oncotarget. 2016.10; 7(41); 67251-67265
19. Saori R Yoshii, Akiko Kuma, Takumi Akashi, Taichi Hara, Atsushi Yamamoto, Yoshitaka Kurikawa,
Eisuke Itakura, Satoshi Tsukamoto, Hiroshi Shitara, Yoshinobu Eishi, Noboru Mizushima. Systemic
Analysis of Atg5-Null Mice Rescued from Neonatal Lethality by Transgenic ATG5 Expression in Neurons.
Dev. Cell. 2016.10; 39(1); 116-130
20. Kiichiro Tsuchiya, Ryohei Hayashi, Keita Fukushima, Shuji Hibiya, Nobukatsu Horita, Mariko Negi,
Eisaku Itoh, Takumi Akashi, Yoshinobu Eishi, Satoshi Motoya, Yoshiaki Takeuchi, Reiko Kunisaki, Ken
Fukunaga, Shiro Nakamura, Naoki Yoshimura, Masakazu Takazoe, Bunei Iizuka, Yasuo Suzuki, Masakazu
Nagahori, Mamoru Watanabe. CDX2 expression induced by leukocytapheresis might be associated withmucosal healing in ulcerative colitis. J. Gastroenterol. Hepatol.. 2016.11;
21. Jiro Akimoto, Kenta Nagai, Daisuke Ogasawara, Yujiro Tanaka, Hitoshi Izawa, Michihiro Kohno, Keisuke
Uchida, Yoshinobu Eishi. Solitary tentorial sarcoid granuloma associated with Propionibacterium acnes
infection: case report. J. Neurosurg.. 2016.11; 1-4
22. Shohei Tomii, Takumi Akashi, Noboru Ando, Tomoki Tamura, Akira Sakurai, Asami Terada, Asuka
Furukawa, Yoshimi Suzuki, Kou Kayamori, Kei Sakamoto, Hironori Ishibashi, Yoshinobu Eishi. Cortical
Actin Alteration at the Matrix-Side Cytoplasm in Lung Adenocarcinoma Cells and Its Signiﬁcance inInvasion. Pathobiology. 2016.12;― 385 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced

23. Nobuyasu Awano, Minoru Inomata, Soichiro Ikushima, Daisuke Yamada, Masatoshi Hotta, Shunji Tsukuda,
Toshio Kumasaka, Tamiko Takemura, Yoshinobu Eishi. Histological analysis of vasculopathy associatedwith pulmonary hypertension in combined pulmonary ﬁbrosis and emphysema: comparison with idiopathic
pulmonary ﬁbrosis or emphysema alone. Histopathology. 2016.12;[Conference Activities & Talks]
1. Yoshinobu EISHI. ABC method clinical trial protocol. Thai gastric cancer society meeting 2016.08.22
Bangkok, Thailand― 386 ―

Advanced Therapeutic Sciences― 387 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

Signal Gene Regulation

Professor NAKAMURA Masataka
Associate Professor FUNATO Noriko
Assistant Professor MIZUGUCHI Mariko(1)

Research

1) Molecular mechanism of tumorigenesis by human T-cell leukemia virus type I (HTLV-I).
2) Roles of transcription factors in cell diﬀeretiation.
3) Implication of prostaglandin D2 receptor (CRTH2) in allergy reactions.
(2)

Lectures & Courses

The aim of Human Gene Sciences Center is to provide laboratory equipment, room and information for researches
in advanced molecular and cellular biology. In educational objectives in the graduate school, our Center gives
lecture, seminar, training course and individual assistance in research ﬁelds of molecular genetics, immunology
and virology.
(3)

Publications[Original Articles]
1. Yosuke Motai, Masahiko Takahashi, Takayuki Takachi, Masaya Higuchi, Toshifumi Hara, Mariko Mizuguchi,
Yutaka Aoyagi, Shuji Terai, Yuetsu Tanaka, Masahiro Fujii. Human T-cell leukemia virus type 1 (HTLV1) Tax1 oncoprotein but not HTLV-2 Tax2 induces the expression of OX40 ligand by interacting with
p52/p100 and RelB. Virus Genes. 2016.02; 52(1); 4-13
2. Mariko Mizuguchi, Yuka Sasaki, Toshifumi Hara, Masaya Higuchi, Yuetsu Tanaka, Noriko Funato, Nobuyuki
Tanaka, Masahiro Fujii, Masataka Nakamura. Induction of Cell Death in Growing Human T-Cells andCell Survival in Resting Cells in Response to the Human T-Cell Leukemia Virus Type 1 Tax. PLoS ONE.
2016.02; 11(2); e0148217
3. Tomoaki Kumagai, Nozomi Yamaguchi, Hiroyuki Hirai, Shigeyuki Kojima, Yoshiko Kodani, Akihiko
Hashiguchi, Michiko Haida, Masataka Nakamura. Loxoprofen sodium induces the production of complement C5a in human serum. Int. Immunopharmacol.. 2016.02; 33; 55-62
4. Lindsay E Laurie, Hiroki Kokubo, Masataka Nakamura, Yumiko Saga, Noriko Funato. The transcription
factor Hand1 is involved in Runx2-Ihh-regulated endochondral ossiﬁcation PLoS ONE. 2016.02; 11(2);e0150263
5. Noriko Funato, Hiroki Kokubo, Masataka Nakamura, Hiromi Yanagisawa, Yumiko Saga. Speciﬁcation of
jaw identity by the Hand2 transcription factor. Sci Rep. 2016.06; 6; 28405
6. Noriko Funato, Hiroki Kokubo, Yumiko Saga. Transcriptomic analyses of Hand2 transgenic embryos.
Genom Data. 2016.09; 9; 60-62― 388 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

7. Deping Kong, Yujun Shen, Guizhu Liu, Shengkai Zuo, Yong Ji, Ankang Lu, Masataka Nakamura, Michael
Lazarus, Constantine A Stratakis, Richard M Breyer, Ying Yu. PKA regulatory II α subunit is essential
for PGD2-mediated resolution of inﬂammation. J. Exp. Med.. 2016.09; 213(10); 2209-2226
8. Yusuke Onaka, Norihito Shintani, Takanobu Nakazawa, Takuya Kanoh, Yukio Ago, Toshio Matsuda,
Ryota Hashimoto, Kazutaka Ohi, Hiroyuki Hirai, Kin-Ya Nagata, Masataka Nakamura, Atsushi Kasai, Atsuko Hayata-Takano, Kazuki Nagayasu, Kazuhiro Takuma, Asao Ogawa, Akemichi Baba, Hitoshi
Hashimoto. Prostaglandin D2 signaling mediated by the CRTH2 receptor is involved in MK-801-induced
cognitive dysfunction. Behav. Brain Res.. 2016.11; 314; 77-86[Conference Activities & Talks]
1. Funato N. The role of transcription factors in bone development. Nippi Research Institute of Biomatrix
2016.07.11
2. Mariko Mizuguchi, Toshifumi Hara, Manami Yoshita-Takahashi, Yuetsu Tanaka, Takuya Fukushima,
Masataka Nakamura. DNA methylation of the human telomerase reverse transcriptase (hTERT) promoter
in human T-cell leukema virus type-1 (HTLV-1) infected T-cells. The 5th JCA-AACR special joint
conference 2016.07.13
3. Funato N. Hand2 transforms upper jaw to lower jaw by regulating the expression of homeobox transcription
factors. The 58th Annual Meeting of Japanese Association for Oral Biology 2016.08.25― 389 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

Material Biofunctions

Akiko Nagai (Associate Professor)
Kosuke Nozaki (Assistant Professor)(1)

Outline

1. Reaction mechanism between materials and living tissues
Development of standard evaluation process for the safety, biocompatibility, and biofunction of biomedical
materials and devices, based on interfacial reactions between the materials and living tissue.
2. Eﬀects of medical materials and devices on human health
Systematization of inﬂuences of biofunctional materials and artiﬁcial organs on the human body from theviewpoint of medicine.
3. Development of functional materials accelerating bone formation
Development of new surface modiﬁcation processes of bioimplants to accelerate bone formation.
4. Development of evaluation methods for studying interaction of materials with biological function
Development of new intravital observation methods for evaluating biological eﬀects of physical and chemicalfactors.
(2)

Education

The objective and principle of this graduate course is to educate students with materials knowledge demanded to
medical and dental doctors who are leading medical professionals and bioscientists who are capable of carryingout their own research at an international level in the area of their special ﬁelds of science, respectively.
(3)

Publications[Original Articles]
1. Hattori T, Igarashi K, Miyazaki H, Aizawa M, Katayama K, Yamashita K, Nagai A. An electrostatic ﬁeld
on a bioceramic growth surface suppressed leiomyosarcoma cell proliferation but accelerated diﬀerentiation
International Journal of Metallurgical & Materials Engineering. 2016; 2; 127
2. Norio Wada, Naohiro Horiuchi, Kastuyuki Mukougawa, Kosuke Nozaki, Miho Nakamura, Akiko Nagai,
Toshinori Okura, and Kimihiro Yamashita. Electrostatic induction power generator using hydroxyapatite
ceramic electrets Materials Research Bulletin. 2016.02; 74; 50-56
3. Naohiro Horiuchi, Norio Wada, Kosuke Nozaki, Miho Nakamura, Akiko Nagai, and Kimihiro Yamashita.
Dielectric relaxation in monoclinic hydroxyapatite: Observation of hydroxide ion dipoles Journal of Applied Physics. 2016.02; 119(8); 084903-6
4. Chen P, Nagai A, Tsutsumi Y, Ashida M, Doi H, Hanawa T . Diﬀerences in the calciﬁcation of preosteoblast
cultured on sputter-deposited titanium, zirconium, and gold J Biomed Mater Res A. 2016.03; 104(3); 639651― 390 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

5. Fukuhara Y, Kyuzo M, Tsutsumi Y, Nagai A, Chen P, Hanawa, T. Phospholipid polymer electrodepositedon titanium inhibits platelet adhesion J. Biomed. Mater. Res. B Appl. Biomater.. 2016.04; 104(3); 554560
6. Ana Milheiro, Kosuke Nozaki, Cornelis J Kleverlaan, Joris Muris, Hiroyuki Miura, Albert J Feilzer. In
vitro cytotoxicity of metallic ions released from dental alloys. Odontology. 2016.05; 104(2); 136-142[Books etc]
1. N. Horiuchi, N. Wada, M. Nakamura, A. Nagai and K. Yamashita. Handbook of Solid State Chemistry.
Wiley, 2016
[Conference Activities & Talks]
1. Iwata N, Nozaki K, Miura H, Yamashita K, Nagai A. Surface modiﬁcation of Ti implant with controlled
micro/nano-structured topography. AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los Angeles,
CA, USA
2. Risa Yamada, Kosuke Nozaki, Hiroyuki Miura, Kimihiro Yamashita and Akiko Nagai. Antimicrobial activity of silver nanoparticle-coated yttria-stabilized zirconia. AADR/CADR Annual Meeting & Exhibition
2016.03.17 Los Angeles, CA, USA
3. Minamifuchi.M, Nemoto.R, Inagaki.T, Uraba.A, Nozaki.K and Miura.H. Inﬂuence of framework design
on shear stress distribution . AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los Angeles, CA,
USA
4. Uraba A, Inagaki T, Nemoto R, Minamifuchi M, Nozaki K, Miura H. The shear stress distribution of two
diﬀerent cantilever RBFPDs. AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los Angeles, CA,USA
5. Fujita K, Nozaki K, Yamashita K, Miura H, and Nagai A. Diﬀerentiation of periodontal ligament cell onhydroxyl- and carbonated-apatites. AADR/CADR Annual Meeting & Exhibition 2016.03.19 Los Angeles,
CA, USA6. Nozaki K, Fujita K, Miura H, Yamashita K, Nagai A. Bone remodeling-optimization of carbonated apatite
for guided bone regeneration. AADR/CADR Annual Meeting & Exhibition 2016.03.19 Los Angeles, CA,USA
7. Akiko Nagai, Takaaki Masutani, Kosuke Nozaki, Kimihiro Yamashita. Electric stimulation induced bypolarized hydroxyapatite regulates osteoblast proliferation through ERK activation. 10th World Biomaterials Congress 2016.05.18
8. Kosuke Nozaki, Kazuhisa Fujita, Naohiro Horiuchi, Miho Nakamura, Hiroyuki Miura, Kimihiro Yamashita, Akiko Nagai. In vivo evaluation of the role of carbonate ion in hydroxyapatite for bone remodeling. 10th World Biomaterials Congress 2016.05.20
9. Fabrication and evaluation of yttria-stabilized zirconia with antimicrobial properties. 2016.07.09
10. Osteoblast behavior on controlled micro/nano structured titanium surface. 2016.07.09
11. Alkaline Phosphatase Activity of Periodontal Ligament Cell on Collagen-coated Hydroxyl-Apatites. 2016.07.09
12. In vivo evaluation of calcium phosphate with optimized osteoconductivity and solubility by carbonate ion.
2016.07.10
13. Fabrication of antibacterial yttria-stabilized zirconia using silver nanoparticle . 2016.09.09
14. Naohiro Horiuchi, Kosuke Nozaki, Miho Nakamura, Akiko Nagai, Kimihiro Yamashita. Phase transitionin calcium hydroxyapatite observed by thermally stimulated current method. The 9th International Symposium on Inorganic Phosphate Materials (ISIPM-9) 2016.09.26 Tokyo Metropolitan University, Tokyo,Japan― 391 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

15. Kosuke Nozaki, Risa Yamada, Hiroyuki Miura, Kimihiro Yamashita, Akiko Nagai. Antibacterial activityof silver nanoparticles coating on zirconia against oral bacteria. The 1st International Symposium on
Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development
2016.10.17
16. Naohiro Horiuchi, Norio Wada, Kosuke Nozaki, Miho Nakamura, Akiko Nagai, and Kimihiro Yamashita.
Surface charges on hydroxyapatite electrets studied by Kelvin probe method. The 28th Annual Meeting of
the International Society for Ceramics in Medicine (Bioceramics 28) 2016.10.20 Charlotte, North Carolina,
USA
17. Akiko Nagai. Carbonate Substitution in Hydroxyapatite Inﬂuences on Macrophage Diﬀerentiation. K-J
Ceramics 33 2016.11.17 Daejeon,Korea― 392 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

Genetic Regulation

Professor Akinori KIMURA
Associate Professor Takeharu HAYASHI
Assistant Professor Jianbo AN
Research Associate Taeko K. NARUSE(1)OutlineResearch and Education in the Department of Genetic Regulation are carried out by staﬀ members of the
Department of Molecular Pathogenesis, Medical Research institute.
The main purpose of this Department is to decipher the genetic regulation involved in the pathogenesis of
intractable diseases, including hereditary cardiovascular diseases such as hypertrophic cardiomyopathy, dilated
cardiomyopathy and hereditary arrhythmia, autoimmune diseases such as rheumatoid arthritis, type I diabetes
mellitus, autoimmune thyroid diseases (Graves disease and Hashimoto thyroiditis), inﬂammatory bowel diseases(Crohn disease and ulcerative colitis) and SLE, as well as infectious diseases including HIV/AIDS. We also
investigate molecular pathogenesis of coronary heart disease (atherosclerosis) and intractable vascular diseases(Takayasu arteritis and Buerger disease).
(2)

Research

1) Identiﬁcation and functional analysis of disease-related genes for cardiovascular diseases including hereditary
cardiomyopathy and arrhythmia
2) Identiﬁcation and functional analysis of disease-related genes for autoimmune diseases including autoimmune
thyroiditis and inﬂammatory bowel disease3) Identiﬁcation and functional analysis of disease-related genes for infectious diseases including HIV-1 infection4) Structural, functional and evolutionary analyses of MHC and immune-related genes
5) Evolutionary medicine for human diseases(3)

Education

Structural and functional diversity of human genome, are involved in the etiology and pathogenesis of humandiseases. Main objective of Genetic Regulation is to identify the gene mutations or polymorphisms and todecipher the molecular mechanisms involved in the etiology and pathogenesis of intractable diseases, in order todevelop new strategies for diagnosis, treatment and/or prevention of the diseases. Current research is focusedon the intractable cardiovascular diseases (e.g. idiopathic cardiomyopathy, idiopathic arrhythmia, and coronary
heart disease), autoimmune diseases (e.g. Burger disease, Graves disease, and rheumatoid arthritis) and infectious diseases (e.g. HIV/AIDS). In addition, genome diversity in immune-related genes is investigated from theview-point of primate evolution.
(4)

Lectures & Courses

Main purpose is to understand the molecular pathogenesis of various intractable diseases by methodologies inthe ﬁelds of Human Genetics, Genome Medicine, Biochemistry and Cell Biology.

― 393 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced(5)

Publications[Original Articles]
1. Taeko K Naruse, Daisuke Sakurai, Hitoshi Ohtani, Gaurav Sharma, Surendra K Sharma, Madhu Vajpayee,
Narinder K Mehra, Gurvinder Kaur, Akinori Kimura. APOBEC3H polymorphisms and susceptibility to
HIV-1 infection in an Indian population. J. Hum. Genet.. 2016.03; 61(3); 263-265
2. Masayoshi Oikawa, Nobuo Sakamoto, Atsushi Kobayashi, Satoshi Suzuki, Akiomi Yoshihisa, Takayoshi
Yamaki, Kazuhiko Nakazato, Hitoshi Suzuki, Shu-Ichi Saitoh, Yuichirou Kiko, Hajime Nakano, Takeharu
Hayashi, Akinori Kimura, Yasuchika Takeishi. Familial hypertrophic obstructive cardiomyopathy with
the GLA E66Q mutation and zebra body. BMC Cardiovasc Disord. 2016.05; 16; 83
3. Sumire Iseda, Naofumi Takahashi, Hugo Poplimont, Takushi Nomura, Sayuri Seki, Taku Nakane, Midori
Nakamura, Shoi Shi, Hiroshi Ishii, Shota Furukawa, Shigeyoshi Harada, Taeko K Naruse, Akinori Kimura,
Tetsuro Matano, Hiroyuki Yamamoto. Biphasic CD8+ T-Cell Defense in Simian Immunodeﬁciency Virus
Control by Acute-Phase Passive Neutralizing Antibody Immunization. J. Virol.. 2016.07; 90(14); 62766290
4. Hideki Kawai, Shin-Ichiro Morimoto, Yoko Takakuwa, Akihiro Ueda, Ken-Ichi Inada, Masayoshi Sarai,
Takuro Arimura, Tatsuro Mutoh, Akinori Kimura, Yukio Ozaki. Hypertrophic Cardiomyopathy Accompanied by Spinocerebellar Atrophy With a Novel Mutation in Troponin I Gene. Int Heart J. 2016.07;
57(4); 507-510
5. Hiroshi Ishii, Saori Matsuoka, Takushi Nomura, Midori Nakamura, Teiichiro Shiino, Yuko Sato, Naoko
Iwata-Yoshikawa, Hideki Hasegawa, Kazuta Mizuta, Hiromi Sakawaki, Tomoyuki Miura, Yoshio Koyanagi,
Taeko K Naruse, Akinori Kimura, Tetsuro Matano. Association of lymph-node antigens with lower Gagspeciﬁc central-memory and higher Env-speciﬁc eﬀector-memory CD8(+) T-cell frequencies in a macaque
AIDS model. Sci Rep. 2016.07; 6; 30153
6. John M Gregson, Daniel F Freitag, Praveen Surendran, Nathan O Stitziel, Rajiv Chowdhury, Stephen
Burgess, Stephen Kaptoge, Pei Gao, James R Staley, Peter Willeit, Sune F Nielsen, Muriel Caslake,
Stella Trompet, Linda M Polfus, Kari Kuulasmaa, Jukka Kontto, Markus Perola, Stefan Blankenberg,
Giovanni Veronesi, Francesco Gianfagna, Satu Männistö, Akinori Kimura, Honghuang Lin, Dermot F
Reilly, Mathias Gorski, Vladan Mijatovic, , Patricia B Munroe, Georg B Ehret, , Alex Thompson, Maria
Uria-Nickelsen, Anders Malarstig, Abbas Dehghan, , Thomas F Vogt, Taishi Sasaoka, Fumihiko Takeuchi,
Norihiro Kato, Yoshiji Yamada, Frank Kee, Martina Müller-Nurasyid, Jean Ferrières, Dominique Arveiler,
Philippe Amouyel, Veikko Salomaa, Eric Boerwinkle, Simon G Thompson, Ian Ford, J Wouter Jukema,
Naveed Sattar, Chris J Packard, Abdulla Al Shaﬁ Majumder, Dewan S Alam, Panos Deloukas, Heribert
Schunkert, Nilesh J Samani, Sekar Kathiresan, , Børge G Nordestgaard, Danish Saleheen, Joanna Mm
Howson, Emanuele Di Angelantonio, Adam S Butterworth, John Danesh, . Genetic invalidation of LpPLA2 as a therapeutic target: Large-scale study of ﬁve functional Lp-PLA2-lowering alleles. Eur J Prev
Cardiol. 2016.12;[Misc]
1. Toshihiro Tanaka, Akinori Kimura. Cardiovascular genetics. J. Hum. Genet.. 2016.01; 61(1); 1[Conference Activities & Talks]
1. Akinori Kimura, Taeko K. Naruse, Daisuke Sakurai, Jianbo An, Hiroshi Terunuma, Emi E. Nakayama,
Tatsuo Shioda, Gaurav Sharma, Narinder Mehra, Gurvinder Kaur. IkBL regulates susceptibility to HIV-1
infection. The 13th International Congress of Human Genetics 2016.04.04 Kyoto, Japan
2. Taeko K. Naruse, Daisuke Sakurai, Hitoshi Ohtani, Hiroshi Terunuma, Yasumasa Iwatani, Wataru Sugiura,
Gaurav Sharma, Narinder Mehra, Gurvinder Kaur, Akinori Kimura. APOBEC3H polymorphisms areassociated with susceptibility to HIV-1 infection and development of AIDS in Asian populations. The
13th international Congress of Human Genetics 2016.04.04 Kyoto, Japan― 394 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

Applied Gene Medicine

Professor Yoshio MIKI
Associate Professor Akira NAKANISHI
Assistant professor Miho TAKAOKA
Project Assistant Professor Ken MIYAGUCHI
Graduate Student Konuskan Ucar AYSE,
Shun ITO,
Maiko UMEGAKI,Gen SATO
Naoko Otsuka(1)

OutlineSince 1981, cancer has been a top leading cause of death in our country and a novel action is an urgent
social challenge. In Department of Molecular Genetics, we aim to study a basic biology underlying cancer andestablish novel diagnostic and therapeutic modalities based on ﬁndings from the fundamental researches. We
have largely focused on three major research directions to understand the molecular mechanisms of breast cancer
development: 1) Uncovering DNA damage repair function and genome stabilization mechanism, 2) Uncoveringhormone-dependent cellular proliferation, and 3) Determining how the tumor microenvironment contributes tocancer development and progression. Utilizing a wide variety of approaches in genomics, molecular biology,
biochemistry and informatics, we are addressing an integrative understanding of multidisciplinary analyses.

(2)

Research

１．Molecular Mechanisms of Breast Cancer Progression◆ Understanding Molecular Mechanisms of Metastasis, Invasion, Recurrence in Cancer◆ Uncovering Molecular functions of hereditary breast cancer genes, BRCA1 and BRCA2
２．Cancer Genomics Research◆ Cancer Genomics Research with Next-Generation Sequencing◆ Identiﬁcation of Genes Involved in Human Cancer Using Genome-Wide Association Studies
３．Cell Death Signaling in Cancer
４．DNA Damage Repair and Genome Instability in Cancer
５．Hormone-Dependent Breast Cancer Cell Growth
６．Cancer Microenvironment(3)

Education

Our research is directed at understanding the molecular mechanism of carcinogenesis, based on basic molecularcell biology and molecular genetics. We have applied new ﬁndings and information obtained by basic research
to develop the new diagnosis, treatment, and prevention of cancer. Our objective in the graduate course is toprovide students opportunity to study basic science and applied genome science for cancer research.

― 395 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced(4)

Publications[Original Articles]
1. Samir Kumar Pal, Chi Thi Kim Nguyen, Kei-Ichi Morita, Yoshio Miki, Kou Kayamori, Akira Yamaguchi,
Kei Sakamoto. THBS1 is induced by TGFB1 in the cancer stroma and promotes invasion of oral squamouscell carcinoma. J. Oral Pathol. Med.. 2016.02;
2. Junhui Wang, Qianshan Ding, Hiroaki Fujimori, Akira Motegi, Yoshio Miki, Mitsuko Masutani. Lossof CtIP disturbs homologous recombination repair and sensitizes breast cancer cells to PARP inhibitors.
Oncotarget. 2016.02; 7(7); 7701-7714
3. Hiroki Osumi, Eiji Shinozaki, Mitsukuni Suenaga, Satoshi Matsusaka, Tsuyoshi Konishi, Takashi Akiyoshi,
Yoshiya Fujimoto, Satoshi Nagayama, Yosuke Fukunaga, Masashi Ueno, Yoshihiro Mise, Takeaki Ishizawa,
Yosuke Inoue, Yu Takahashi, Akio Saiura, Hirohumi Uehara, Mingyon Mun, Sakae Okumura, Nobuyuki
Mizunuma, Yoshio Miki, Toshiharu Yamaguchi. RAS mutation is a prognostic biomarker in colorectal
cancer patients with metastasectomy. Int. J. Cancer. 2016.03;[Conference Activities & Talks]
1. Hiroko Saito, Miho Takaoka, Akira Nakanishi, Yoshio Miki. Loss of BRCA2 enhances microtubules morestabilization with PTX treatment. Tenth AACR-JCA Joint Conference 2016.02.16 USA― 396 ―

Advanced Therapeutic
Sciences
Medical Genomics

Molecular Cytogenetics

Professor Johji Inazawa M.D., Ph.D.
Lecturer Jun Inoue Ph.D.
Assistant Professor Tomoki Muramatsu Ph.D.
Assistant Professor Yasuyuki Gen M.D., Ph.D.
(1)

Research

1.Identification of genes responsible for intractable diseases including cancer and genomic disorders throughintegrative genomics and epigenomics.
2.Discovery of molecular mechanisms of cancer-related genes, including microRNAs, in the multistep processesof carcinogenesis and cancer progression, such as cancer stem cell, epithelial-mesenchymal transition (EMT),
invasion and metastasis using systems biology.
3.Establishment of autophagy-based diagnosis and therapy in human cancers by understanding cellular contextdependent role of autophagy.
4.Multiple genomic analyses of genetic disorders of unknown etiology, e.g. mental retardation or epilepsy, todetect causative genes and clarify the etiology. Also, an array chip for diagnosis of known congenital disorders,
Genome Disorder Array , was developed and released for a practical use at 2009.
5.Development of innovative techniques for genomics and epigenomics in medical science.
6.Development of practically useful tools for molecular diagnosis of intractable diseases.

(2)

Lectures & Courses

The principal aim of the Department of Molecular Cytogenetics(MCG) is to understand the molecular mechanism underlying intractable diseases, such as cancer and uncharacterized genetic diseases. Main objective of
MCG in the graduate course is to provide students opportunity to study molecular cytogenetic approach for
intractable diseases, identify genes responsible for those diseases, and develop innovative techniques/ practicallyuseful tools for detection of genomic and epigenomic aberrations in those diseases. It is our goal to bridge thegap between basic and clinical research for the benefit of each of the patients.

(3)

Publications[Original Articles]
1. Okada Yukinori, Muramatsu Tomoki, Suita Naomasa, Kanai Masahiro, Kawakami Eiryo, Iotchkova
Valentina, Soranzo Nicole, Inazawa Johji, Tanaka Toshihiro. Significant impact of miRNA-target gene
networks on genetics of human complex traits. Sci Rep. 2016; 6; 22223
2. Maki Morishita, Tomoki Muramatsu, Yumiko Suto, Momoki Hirai, Teruaki Konishi, Shin Hayashi, Daichi
Shigemizu, Tatsuhiko Tsunoda, Keiji Moriyama, Johji Inazawa. Chromothripsis-like chromosomal rearrangements induced by ionizing radiation using proton microbeam irradiation system. Oncotarget.
2016.03; 7(9); 10182-10192― 397 ―

Advanced
Therapeutic Sciences
MedicalGenomics

3. Genki Sudo, Tetsushi Kagawa, Yasuhiro Kokubu, Johji Inazawa, Tetsuya Taga. Increase in GFAP-positive
astrocytes in histone demethylase GASC1/KDM4C/JMJD2C hypomorphic mutant mice. Genes Cells.
2016.03; 21(3); 218-225
4. Daniela Tiaki Uehara, Shin Hayashi, Nobuhiko Okamoto, Seiji Mizuno, Yasutsugu Chinen, Rika Kosaki,
Tomoki Kosho, Kenji Kurosawa, Hiroshi Matsumoto, Hiroshi Mitsubuchi, Hironao Numabe, Shinji Saitoh,
Yoshio Makita, Akira Hata, Issei Imoto, Johji Inazawa. SNP array screening of cryptic genomic imbalancesin 450 Japanese subjects with intellectual disability and multiple congenital anomalies previously negative
for large rearrangements. J. Hum. Genet.. 2016.04; 61(4); 335-343
5. T Muramatsu, K-I Kozaki, S Imoto, R Yamaguchi, H Tsuda, T Kawano, N Fujiwara, M Morishita,
S Miyano, J Inazawa. The hypusine cascade promotes cancer progression and metastasis through theregulation of RhoA in squamous cell carcinoma. Oncogene. 2016.04;
6. Michelle Nuylan, Tatsuyuki Kawano, Johji Inazawa, Jun Inoue. Down-regulation of LAPTM5 in humancancer cells. Oncotarget. 2016.04;
7. Tumurkhuu T, Fujiwara T, Komazaki Y, Kawaguchi Y, Tanaka T, Inazawa J, Ganburged G, Bazar A,
Ogawa T, Moriyama K. Association between maternal education and malocclusion in Mongolian adolescents: a cross-sectional study. BMJ Open. 2016.11; 6(11); e012283[Conference Activities & Talks]
1. Morita K,Naruto T, Tanimoto K, Yasukawa C, Oikawa Y, Masuda K, Imoto I, Inazawa J. Simultaneous
detection of both single nucleotide variations and copy number alterations by nextgeneration sequencing
in Gorlin syndrome.. ICHG2016 2016.04 Kyoto, Japan
2. Kanai M, Okada Y, Muramatsu T, Suita N, Kawakami E, Iotchkova V, Soranzo N, Inazawa J, Tanaka
T. Significant impact of miRNA-target gene networks on genetics of human complex traits. ICHG2016
2016.04 Kyoto, Japan
3. Uehara D.T, S Hayashi, Makita Y ,Hata A, Imoto I ,Inazawa J. Screening of copy number variants in 450
Japanese subjects presenting with intellectual disability (ID) and multiple congenital anomalies (MCA)by SNP array unveiling rare small variants and PPFIA2 as a novel candidate gene for ID. ICHG2016
2016.04.05 Kyoto, Japan
4. Inoue J. MicroRNA-based diagnosis and therapy in NRF2-stabilized tumor. 107th annual meeting ofAmerican Association for Cancer Research 2016 2016.04.18 New Orleans, USA.
5. Muramatsu T, Tanimoto K, Inazawa J. Exploring target genes of eukaryotic initiation factor 5A in cancer
using RNA sequence analysis. 107th annual meeting of American Association for Cancer Research 20162016.04.18 New Orleans, USA.
6. Inoue J, Fujiwara N, Yamamoto S, Kawano T, Inazawa J. MicroRNA-based diagnosis and therapy
in NRF2-stabilized tumor. 107th annual meeting of American Association for Cancer Research 20162016.04.18 New Orleans, USA.

― 398 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

Hematology

Professor Osamu Miura
Professor( Immunotherapy for Hematopoietic Disorders) Norihiko Kawamata
Junior Associate Professor Ayako Arai
Assistant Professor Tetsuya Fukuda, Masahide Yamamoto, Ken Watanabe, Ayako Nogami
Project Assistant Professor Chizuko Sakashita
Senior Resident Tomoko Yamashitai, Tatsuya Saito, Kota Yoshifuji
Graduate Student Yoshihiro Umezawa, Hiroki Akiyama, Shinya Ishida,
Keigo Okada, Emi Uchida, Daisuke Watabae,
Koji Sasaki, Shuhei Fujita(1)

OutlineThe Department of Hematology is responsible for clinical services at our University Hospital with treatmentof patients with various hematological disorders including leukemias, lymphomas, anemia, and hemorrhagic
diseases by chemotherapies, immunotherapies, molecularly-targeted therapies, and hematopoietic cell transplantation. Our department is also responsible for teaching undergraduate students with the lecture course inhematology as well as the clinical clerkship and for training junior and senior residents. Our department is also
actively involved, with doctoral course students, in basic and clinical researches aiming to elucidate the molecular and cellular mechanisms involved in pathogenesis of hematological malignancies as well as in acquisition oftherapy resistance to develop novel eﬃcient therapies against these diseases.
(2)

Research

The research interests and activities of the Department of Hematology are diverse, and the following projects
among others are currently under way. 1. Elucidation of the molecular mechanisms involved in acquisition ofresistance against chemotherapies and molecularly-targeted therapies by leukemic cells from chronic myeloid
leukemia, acute myeloid leukemia, and various myeloproliferative neoplasms expressing aberrant tyrosine kinases, including BCR/ABL, FLT3-ITD, and Jak2-V617F, aiming to develop novel therapeutic strategies toovercome the resistance. 2. Elucidation of the regulation mechanisms for immune responses to indolent lymphoid neoplasms, including chronic lymphocytic leukemia, for developing novel immunotherapies. 3. Elucidationof the pathogenesis of chronic active EB virus infection and development of eﬀective therapeutic modalities.
4. Signal transduction mechanisms from cytokine/growth factor receptors regulating proliferation, survival,
and adhesion of hematopoietic cells including leukemia and lymphoma cells. The Department is also actively
involved in a variety of clinical studies for treatment of various leukemias, lymphomas, and multiple myeloma.
(3)

Education

The Department of hematology is responsible for teaching basic and clinical hematology to the 3rd and 4th
grade students in the integrated hematology course and the hematology and oncology united block course. The― 399 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced

Department is also responsible for teaching the 5th and 6th grade students in clinical clerkship to obtain basic
knowledge and problem-solving abilities in hematology as well as general internal medicine. The Department
is also actively involved in training about 24 junior residents every year to acquire clinical skills in hematology
and internal medicine and about 4 senior residents to practice diagnosis and treatment of various hematologicaldisorders and to obtain the certiﬁcate for hematology specialist. As a division in the Graduate School of
Medical and Dental Sciences, the Department is actively involved in education of 6 to 8 graduate students, whoparticipate in the research projects listed above, to obtain the Ph. D. degree in medicine.
(4)

Lectures & Courses

The major objective of the course is to understand the pathophysiology of blood cells, blood cell-forming organs,and hemostasis to provide a basis for rational diagnosis and treatment of their disorders.
(5)

Clinical Services & Other Works

The Department of Hematology provides diagnosis and treatment for hematological diseases, such as leukemia,
malignant lymphoma, anemia, and thrombocythemia, with chemotherapeutics, molecularly-targeted drugs,
immunosuppressive agents, and hematopoietic cell trans- plantation.
(6)

Clinical Performances

We provide the highest quality of patient care for a wide spectrum of blood diseases and cancers.
(7)

Publications[Original Articles]
1. Yui S, Yamaguchi H, Imadome K, Arai A, Takahashi M, Ohashi R, Asayama T, Kondo A, Moriya K,
Nakayama K, Dan K, Shimizu S, Inokuchi K.. Epstein-Barr virus positive T cell lymphoproliferative disease following cord blood transplantation for acute myelogenous leukemia The Journal of Nippon Medical
School. 2016;
2. Nobuo Sanjo,Satoko Kina,Yukiko Shishido-Hara,Yurie Nose,Satoru Ishibashi,Tetsuya Fukuda,Taketoshi
Maehara,Yoshinobu Eishi,Hidehiro Mizusawa,Takanori Yokota. A Case of Progressive Multifocal Leukoencephalopathy with Balanced CD4/CD8 T-Cell Inﬁltration and Good Response to Meﬂoquine Treatment.
Internal Medicine. 2016.01; 55(12); 1631-1635
3. Uchida Emi, Watanabe Ken, Oshikawa Gaku, Sakasita Chizuko, Kurosu Tetsuya, Fukuda Tetsuya, Arai
Ayako, Murakami Naoki, Miura Osamu, Yamamoto Masahide. Refractory primary myeloid sarcoma of
the breast with MLL-AF9 rearrangement. The Japanese Journal of Clinical Hematology. 2016.01; 57(1);4. Arai A, Sakashita C, Hirose C, Imadome K, Yamamoto M, Jinta M, Fujiwara S, Tomita M, Shimizu
N, Morio T, and Miura O.. Hematopoietic stem cell transplantation for adults with EBV-positive Tor NK-cell lymphoproliferative disorders: eﬃcacy and predictive markers Bone Marrow Transplantation.
2016.02;
5. Naoyuki Miyasaka, Osamu Miura, Tatsuya Kawaguchi, Nobuyoshi Arima, Eriko Morishita, Kensuke
Usuki, Yasuyoshi Morita, Kaichi Nishiwaki, Haruhiko Ninomiya, Akihiko Gotoh, Shinsaku Imashuku,
Akio Urabe, Tsutomu Shichishima, Jun-Ichi Nishimura, Yuzuru Kanakura. Pregnancy outcomes of patients with paroxysmal nocturnal hemoglobinuria treated with eculizumab: a Japanese experience andupdated review. Int. J. Hematol.. 2016.02;
6. Shunsuke Yui, Hiroki Yamaguchi, Ken-ichi Imadome, Ayako Arai, Mikiko Takahashi, Ryuji Ohashi, Hayato
Tamai, Keiichi Moriya, Kazutaka Nakayama, Akira Shimizu, Koiti Inokuchi. Epstein-Barr Virus-positive
T-cell Lymphoproliferative Disease Following Umbilical Cord Blood Transplantation for Acute Myeloid
Leukemia. J Nippon Med Sch. 2016.02; 83(1); 35-42― 400 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

7. Megumi Akiyama, Kota Yoshifuji, Tetsuya Fukuda, Shuji Tohda, Tohru Miki, Osamu Miura, Masahide
Yamamoto. Fulminant visceral disseminated varicella-zoster virus infection without skin involvement ina patient with autoimmune hemolytic anemia on prednisolone therapy. Rinsho Ketsueki. 2016.04; 57(4);467-471
8. Shunichiro Yasuda, Masahide Yamamoto, Tetsuya Fukuda, Yasufumi Ohtsuka, Osamu Miura. Postoperative Atypical Hemolytic Uremic Syndrome Treated Successfully with Eculizumab. Intern. Med.. 2016.05;
55(9); 1171-1175
9. Masahide Yamamoto, Kazuteru Ohashi, Kazuhiko Kakihana, Yuichi Nakamura, Takuya Komeno, Hiroshi
Kojima, Satoshi Morita, Hisashi Sakamaki. A multicenter clinical study to determine the feasible initial
dose of lenalidomide for maintenance therapy in patients with multiple myeloma following autologous
peripheral blood stem-cell transplantation. Mol Clin Oncol. 2016.06; 4(6); 965-970
10. Yoshihiro Umezawa, Tetsuya Kurosu, Hiroki Akiyama, Nang Wu, Ayako Nogami, Toshikage Nagao,
Osamu Miusa. Down regulation of Chk1 by p53 plays a role in synergistic induction of apoptosis bychemotherapeutics and inhibitors for Jak2 or BCR/ABL in hematopoietic cells. Oncotarget. 2016.06;
11. Yuki Shirai, Mitsunori Miyashita, Masako Kawa, Toru Motokura, Fumiaki Sano, Tetsuya Fukuda, Kazuo
Oshimi, Keiko Kazuma. Evaluation of care for leukemia and lymphoma patients during their last hospitalization from the perspective of the bereaved family. Leuk. Res.. 2016.08; 47; 93-99
12. Hiroki Akiyama, Hiroshi Takase, Fumito Kubo, Tohru Miki, Masahide Yamamoto, Makoto Tomita, Manabu Mochizuki, Osamu Miura, Ayako Arai. High-dose methotrexate following intravitreal methotrexate
administration in preventing central nervous system involvement of primary intraocular lymphoma. Cancer Science. 2016.10; 107(10); 1458-1464
13. Shunichiro Yasuda, Keisuke Tanaka, Ayako Ichikawa, Ken Watanabe, Emi Uchida, Masahide Yamamoto,
Kouhei Yamamoto, Daisuke Mizuchi, Osamu Miura, Tetsuya Fukuda. Aggressive TAFRO syndrome withreversible cardiomyopathy successfully treated with combination chemotherapy. Int. J. Hematol.. 2016.10;
104(4); 512-518
14. Koichi Miyamura, Toshihiro Miyamoto, Mitsune Tanimoto, Kazuhito Yamamoto, Shinya Kimura, Tatsuya Kawaguchi, Itaru Matsumura, Tomoko Hata, Hisashi Tsurumi, Shigeki Saito, Masayuki Hino, Seiji
Tadokoro, Kuniaki Meguro, Hideo Hyodo, Masahide Yamamoto, Kohmei Kubo, Junichi Tsukada, Midori Kondo, Makoto Aoki, Hikaru Okada, Masamitsu Yanada, Kazuma Ohyashiki, Masafumi Taniwaki.
Switching to nilotinib in patients with chronic myeloid leukemia in chronic phase with molecular suboptimal response to frontline imatinib: SENSOR ﬁnal results and BIM polymorphism substudy. Leuk. Res..
2016.12; 51; 11-18[Misc]
1. Ayako Arai. The issues of chronic active Epstein-Barr virus infection 2016.09; 73(3); 400-404[Conference Activities & Talks]
1. The impact of 2nd autologous stem cell transplantation (ASCT) in patients with multipe myeloma who
relapsed after 1st ASCT. The 38th Annual Meeting of the Japan Society for Hematopoietic Cell Transplantation 2016.03.05
2. Haruna Shibayama, Ken-ichi Imadome, Chizuko Sakashita, Ken Watanabe, Norio Shimizu, Takatoshi
Koyama, Shigeyoshi Fujiwara, Osamu Miura, Ayako Arai. In vitro and in vivo eﬀects of proteasome
inhibitor bortezomib on Epstein-Barr virus-positive T- or NK-cell lymphoproliferative diseases. 17th
International Symposium on Epstein-Barr virus (EBV) and Associated Diseases 2016.08.10 Zurich
3. Yujin Sekinaka, Noriko Mitsuiki, Kohsuke Imai, Miharu Yabe, Hiromasa Yabe, Masatoshi Takagi, Ayako
Arai, Kenichi Yoshida, Yusuke Okuno, Yuichi Shiraishi, Kenichi Chiba, Hiroko Tanaka, Satoru Miyano,
Seiji Kojima, Asuka Hira, Minoru Takata, Osamu Ohara, Seishi Ogawa, Tomohiro Morio, and Shigeaki
Nonoyama.. Common variable immunodeﬁciency caused by FANC mutations.. 17th Biennial Meeting ofEuropean Society for Immunodeﬁciencie. 2016.09 Barcelona― 401 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced

4. Ayako Nogami, Keigo Okada, Gaku Oshikawa, Shinya Ishida, Hiroki Akiyama, Yoshihiro Umezawa, Tetsuya Kurosu, Osamu Miura. FLT3-ITD Confers Resistance to Bortezomib by Protecting the mTOR
Pathway via the Pim Kinases in AML. The 78th Annual Meeting of the Japanese Society of Hematology
2016.10.13
5. Yoshihiro Umezawa, Hiroki Akiyama, Keigo Okada, Shinya Ishida, Ayako Nogami, Gaku Oshikawa, Tetsuya Kurosu, Osamu Miura. PECAM-1 enhances SDF-1-induced chemotaxis mediated through activationof the PI3K/Akt/mTORC1 pathway. The 78th annual meeting of the Japanese society of hematology
2016.10.14 Yokohama,Japan
6. Haruna Shibayama, Ken-ichi Imadome, Chizuko Sakashita, Ken Watanabe, Fuyuko Kawano, Norio Shimizu,
Takatoshi Koyama, Shigeyoshi Fujiwara, Osamu Miura, Ayako Arai. In vitro and in vivo eﬀects of bortezomib on EBV-T/NK-LPDs. The 78th Annual Meeting of the Japanese Society of Hematology 2016.10.14
Yokohama
7. Hiroki Akiyama,Yoshihiro Umezawa,Keigo Okada,Shinya Ishida,Ayako Nogami,Gaku Oshikawa,Osamu
Miura. Deubiquitinase inhibitor WP1130 blocks JAK2-V617F and FLT3-ITD to induce apoptosis inleukemic cells. The 78th Annual Meeting of the Japanese Society of Hematology 2016.10.15 Yokohama― 402 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

Molecular Endocrinology and Metabolism

Professor: Yoshihiro Ogawa
Associate Professor: Takanobu Yoshimoto, Hajime Izumiyama
Assistant Professor: Isao Minami, Kyoichiro Tsuchiya, Ryotaro Bouchi
Clinical Fellow: Eri Ueda, Yusuke Matsuda, Hideyuki Okuma, Yoshihiro Niitsu
Resident: Seizaburo Matsuda, Asuka Shinmei
Project Assistant Professor: Misa Saijo, Masanori Murakami, Takeru Sakai, Xunmei Yuan1 ,Michiko Shirakawa1 , Ibuki Shirakawa1
Graduate Students (Doctor s course): Kazutaka Tsujimoto, Yasutaka Miyachi, Kumiko Shiba,
Kenichi Kawahori, Maki Kawasaki, Toshihiro Goto, Takuya Ohmura, Megumi Hatano, Yujiro Nakano,
Takato Takeuchi, Masahiro Asakawa, Nozomi Hanzawa, Kentaro Mori, Tatsuya Fukuda
Project Associate Professor: Koshi Hashimoto2 , Kyoko Shirakabe1
1(1)

Department of Organ Network and Metabolism, 2 Department of Preemptive Medicine and Metabolism

Outline1. Purpose of Education
Our training program enables postdoctoral trainees to prepare for the future academic careers and the clinicalpractice in the broad discipline of endocrinology and metabolism. The research program provides mentor-based
training in experimental design, laboratory and clinical research techniques and methodology, and interpretation
and analysis of the results obtained from cellular and molecular biology, physiology, clinical physiology, clinicaltherapeutics, and health sciences. This training program is designed to educate and establish physicianscientist in the ﬁeld of endocrinology and metabolism.
2. Research Subjects
1) Role of adipose tissue inﬂammation in the metabolic syndrome2) Molecular mechanisms of saturated fatty acid-induced chronic inﬂammation
3) Molecular mechanism of vascular injury in diabetes, endocrine and metabolic diseases4) Role of epigenetic regulation in metabolism
5) Mechanism of pathogenesis in endocrine tumors6) Development of novel diagnostic and therapeutic tools in endocrine and metabolic diseases
3. Clinical Services
Comprehensive inpatient and outpatient services in the area of endocrine and metabolic disorders, including:1) diseases of the thyroid, pituitary and adrenal glands.
2) diabetes mellitus, diabetic complications, metabolic syndrome, and obesity3) primary and secondary hypertension4) disorders of calcium metabolism― 403 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced(2)

Publications[Original Articles]
1. Ryotaro Bouchi, Masahiro Asakawa, Norihiko Ohara, Yujiro Nakano, Takato Takeuchi, Masanori Murakami, Yuriko Sasahara, Mitsuyuki Numasawa, Isao Minami, Hajime Izumiyama, Koshi Hashimoto,Takanobu Yoshimoto, Yoshihiro Ogawa. Indirect measure of visceral adiposity ’A Body Shape Index’(ABSI) is associated with arterial stiﬀness in patients with type 2 diabetes. BMJ Open Diabetes Res
Care. 2016; 4(1); e000188
2. Chikara Komiya, Kyoichiro Tsuchiya, Kumiko Shiba, Yasutaka Miyachi, Shunsaku Furuke, Noriko Shimazu, Shinobu Yamaguchi, Kazuo Kanno, Yoshihiro Ogawa. Ipragliﬂozin Improves Hepatic Steatosis in
Obese Mice and Liver Dysfunction in Type 2 Diabetic Patients Irrespective of Body Weight Reduction.
PLoS ONE. 2016; 11(3); e0151511
3. Koshi Hashimoto, Tetsuya Tagami, Hajime Yamakage, Kazuya Muranaka, Masashi Tanaka, Shinji Odori,
Shigeo Kono, Akira Shimatsu, Yoshihiro Ogawa, Noriko Satoh-Asahara. Serum free thyroxine levels areassociated with the eﬃcacy of weight reduction therapy in obese female patients. Endocr. J.. 2016; 63(3);221-229
4. Ryotaro Bouchi, Norihiko Ohara, Masahiro Asakawa, Yujiro Nakano, Takato Takeuchi, Masanori Murakami, Yuriko Sasahara, Mitsuyuki Numasawa, Isao Minami, Hajime Izumiyama, Koshi Hashimoto,Takanobu Yoshimoto, Yoshihiro Ogawa. Is visceral adiposity a modiﬁer for the impact of blood pressure
on arterial stiﬀness and albuminuria in patients with type 2 diabetes? Cardiovasc Diabetol. 2016.01; 15;105. Sumiyo Morita, Kazuhiko Nakabayashi, Tomoko Kawai, Keiko Hayashi, Takuro Horii, Mika Kimura,
Yasutomi Kamei, Yoshihiro Ogawa, Kenichiro Hata, Izuho Hatada. Gene expression proﬁling of white
adipose tissue reveals paternal transmission of proneness to obesity. Sci Rep. 2016.02; 6; 21693
6. Ryotaro Bouchi, Yujiro Nakano, Norihiko Ohara, Takato Takeuchi, Masanori Murakami, Masahiro Asakawa,
Yuriko Sasahara, Mitsuyuki Numasawa, Isao Minami, Hajime Izumiyama, Koshi Hashimoto, Takanobu
Yoshimoto, Yoshihiro Ogawa. Clinical relevance of dual-energy X-ray absorptiometry (DXA) as a simultaneous evaluation of fatty liver disease and atherosclerosis in patients with type 2 diabetes. Cardiovasc
Diabetol. 2016.04; 15; 64
7. Tatsuya Fukuda, Ryotaro Bouchi, Isao Minami, Norihiko Ohara, Yujiro Nakano, Rie Nishitani, Masanori
Murakami, Takato Takeuchi, Momoko Akihisa, Masamichi Fujita, Hajime Izumiyama, Koshi Hashimoto,Takanobu Yoshimoto, Yoshihiro Ogawa. Retrograde pyelonephritis and lumbar spondylitis as a result of
Salmonella typhi in a type 2 diabetes patient with neurogenic bladder. J Diabetes Investig. 2016.05;
7(3); 436-439
8. Rumi Hachiya, Takuya Shiihashi, Ibuki Shirakawa, Yorihiro Iwasaki, Yoshihiro Matsumura, Yumiko Oishi,
Yukiteru Nakayama, Yoshihiro Miyamoto, Ichiro Manabe, Kozue Ochi, Miyako Tanaka, Nobuhito Goda,
Juro Sakai, Takayoshi Suganami, Yoshihiro Ogawa. The H3K9 methyltransferase Setdb1 regulates TLR4mediated inﬂammatory responses in macrophages. Sci Rep. 2016.06; 6; 28845
9. Takanori Chiba, Erika Ishihara, Norio Miyamura, Rika Narumi, Mihoko Kajita, Yasuyuki Fujita, Akira
Suzuki, Yoshihiro Ogawa, Hiroshi Nishina. MDCK cells expressing constitutively active Yes-associatedprotein (YAP) undergo apical extrusion depending on neighboring cell status. Sci Rep. 2016.06; 6; 28383
10. Masahiro Asakawa, Takanobu Yoshimoto, Mitsutane Ota, Mitsuyuki Numasawa, Yuriko Sasahara, Takato
Takeuchi, Yujiro Nakano, Norihiko Oohara, Masanori Murakami, Ryotaro Bouchi, Isao Minami, Kyoichiro Tsuchiya, Koshi Hashimoto, Hajime Izumiyama, Naoko Kawamura, Kazunori Kihara, Mariko
Negi, Takumi Akashi, Yoshinobu Eishi, Hironobu Sasano, Yoshihiro Ogawa. A Case of Cushing’s Syndrome with Multiple Adrenocortical Adenomas Composed of Compact Cells and Clear Cells. Endocr.
Pathol.. 2016.06; 27(2); 136-141
11. Ryotaro Bouchi, Takato Takeuchi, Momoko Akihisa, Norihiko Ohara, Yujiro Nakano, Rie Nishitani,
Masanori Murakami, Tatsuya Fukuda, Masamichi Fujita, Isao Minami, Masatomo Mihara, Takanobu
Yoshimoto, Yoshihiro Ogawa. Increased visceral adiposity with normal weight is associated with theprevalence of non-alcoholic fatty liver disease in Japanese patients with type 2 diabetes. J Diabetes
Investig. 2016.07; 7(4); 607-614― 404 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

12. Masanori Murakami, Takanobu Yoshimoto, Yujiro Nakano, Kyoichiro Tsuchiya, Isao Minami, Ryotaro
Bouchi, Yasuhisa Fujii, Kazuhiko Nakabayashi, Koshi Hashimoto, Ken-Ichiro Hata, Kazunori Kihara,
Yoshihiro Ogawa. Expression of inﬂammation-related genes in aldosterone-producing adenomas withKCNJ5 mutation. Biochem. Biophys. Res. Commun.. 2016.08; 476(4); 614-619
13. Richard Thomas Jennings, Erdenezaya Odkhuu, Akina Nakashima, Naoko Morita, Toshihiko Kobayashi,
Ikuko Yamai, Miyako Tanaka, Takayoshi Suganami, Sanae Haga, Michitaka Ozaki, Yasuharu Watanabe,
Yoshinori Nagai, Kiyoshi Takatsu, Takane Kikuchi-Ueda, Isao Ichimonji, Yoshihiro Ogawa, Hidekazu
Takagi, Tatsuya Yamazaki, Kensuke Miyake, Sachiko Akashi-Takamura. Inﬂammatory responses increase
secretion of MD-1 protein. Int. Immunol.. 2016.10; 28(10); 503-512
14. Megumi Hatano, Toshiro Migita, Tomokazu Ohishi, Yuichi Shima, Yoshihiro Ogawa, Ken-Ichirou Morohashi, Yukihiro Hasegawa, Futoshi Shibasaki. SF-1 deﬁciency causes lipid accumulation in Leydig cells
via suppression of STAR and CYP11A1. Endocrine. 2016.11; 54(2); 484-496― 405 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

Hepatobiliary and Pancreatic Surgery

Director & Professor
Minoru Tanabe MD, PhD
LecturerAtsushi Kudo MD, PhD
Assistant Professor
Takanori Ochiai MD, PhD
Daisuke Ban MD, PhD
Arihiro Aihara MD, PhD （until September）
Hiroaki Ono MD, PhD (since March)
Satoshi Matsumura MD, PhD
Yusuke Mitsunori MD, PhD
Satoshi Matsumura MD, PhD
Hiromitsu Ito MD (until March)
Keiichi Akakhoshi MD, PhD (since October)
Yasuhito Iwao MD, PhD (since April)
Graduate School Students
Keiichi Akakhoshi MD (until March)
Yasuhito Iwao MD (until March)
Hiroki Ueda MD (until September)
Yoshiteru Ohata MD (until March)
Atsushi Oba MD (until March)
Taku Sato MD
Yuki Mizuno MD
Norimichi Chiyonobu MD
Haku Liu MD
Shuichi Watanabe MD
Yoshiya Ishikawa MD
Daisuke Asano MD (joining April)
Jun Yoshino MD (joining April)(1)

Outline

The department of Hepato-Biliary-Pancreatic Surgery at Tokyo Medical and Dental University focus on theliver, biliary tract and pancreas with benign and malignant disorders. We constantly strive to provide thehighest level of complex and innovative surgical care, comprehensive surgical training for tomorrow’s leaders aswell as groundbreaking basic science and clinical research.
(2)

Research

We conduct medical research in both clinical and laboratory settings and develop novel ideas in research which
impact patient outcomes, teaching, and clinical care.
Our research programs encompass:· Biomolecular mechanisms of carcinogenesis, cancer growth, invasion and metastasis― 406 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences· Molecular target therapy for malignant diseases· Cancer stem cell
· Extended indication for hepatectomy· The system of liver microcirculation· Laparoscopic surgery for hepatobiliary pancreatic diseases· Liver transplantation and organ preservation· Treatments for neuroendocrine tumor· Innovation of imaging modality for hepatobiliary pancreatic diseases(3)

Education

Medical students program:
We conduct the various experiences of hepatobiliary pancreatic diseases, diagnosis and management, throughlectures, pre-clinical clerkship and clinical clerkship. Clinical clerkship exposes students to the surgical patientsand basic surgical techniques. It also provides opportunities to participate in peri-operative care as well as
operative procedures. Students learn interpersonal and communication skills that result in the eﬀective exchange
of information and teaming with patients, their families, and professional associates.
Surgical training program:
The aim of our surgical training program cultivates not only training for certiﬁed board surgeons, but also
the future surgical leaders, through experiences from the academic, the operative, and the outpatient aspectsof management in university hospital and aﬃliated hospitals. Clinically, the trainees receive training andexperience in the preoperative, operative, and post-operative care of patients and basic science and clinicalresearch in our training programs strive to help young surgeons develop both technical and cognitive expertise.
(4)

Clinical Performances

Our highly experienced surgeons oﬀer state-of-the-art diagnosis and treatment, such as the multidisciplinary
treatments (based on radical surgery) for advanced malignant diseases, minimally invasive procedures (including
reduced port surgery).
Annually, 255 operations (hepatectomy: 96 cases, pancreatectomy: 73 cases) were performed in 2014, placing
one of the top high volume medical centers in the country for hepatobiliary pancreatic surgery.
The 325 patients with neuroendocrine tumors were treated in 2016. We have treated the largest number of thepatients with NENs in Japan.
(5)

Publications[Original Articles]
1. E. Soeda, K. Hoshino, Y. Izawa, C. Takaoka, C. Isobe, A. Takahashi, N. Takahashi, Y. Yamada, N.
Shimojima, A. Fujino, M. Shinoda, Y. Kitagawa, M. Tanabe, S. Nakamura, N. Taki, A. Sekiguchi, Y.
Nakazawa, T. Turukawa, and T. Kuroda. A report on the Positive Response to an Outdoor Nature
Challenge of a Snow Camp for Young Liver Transplantation Patients Transplant Proceedings. 2016.01;
49; 115-120
2. M. Shimazu, Y. Kato, S. Kawachi, M. Tanabe, K. Hoshino, G. Wakabayashi, Y. Kitagawa, and M. Kitajima. Impact of Portal Hemodynamic Changes in Partial Liver Grafts on Short-Term Graft Regenerationin Living Donor Liver Transplantation Transplant Proceedings. 2016.01; 48; 2747-2755
3. Masaki Kaibori , Hiroyuki Nitta , Michihiro Hayashi , Shigekazu Takemura , Hiroaki Nagano , Kosuke
Matsui , Hisashi Ikoma , Takuya Nakai , Masafumi Yasunaga , Masahiro Kido , Takeshi Aoki , Toshiki
Rikiyama , Keiji Sano , Atsushi Kudo , Satoshi Katagiri , Yuichiro Otsuka , Tamotsu Kuroki , Takeo
Nomi , Koichi Yano , Itaru Endo , Masaki Ueno , Akishige Kanazawa , Hiroaki Terajima , Saiho Ko , Goro
Honda , Yasuji Seyama , Hiroki Sunagawa , Tsukasa Aibara. Questionnaire survey on work motivationsof gastrointestinal and hepatobiliary pancreatic surgeons enrolled in a Japanese national interdisciplinary
program J Hepatobiliary Pancreat Sci . 2016.01; 23; 697-702― 407 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

4. Katsuta E, Tanaka S, Mogushi K, Shimada S, Akiyama Y, Aihara A, Matsumura S, Mitsunori Y, Ban
D, Ochiai T, Kudo A, Fukamachi H, Tanaka H, Nakayama K, Arii S, Tanabe M.. CD73 as a therapeutictarget for pancreatic neuroendocrine tumor stem cells. Int J Oncol. . 2016.02; 48(2); 657-669
5. Fukiko Kawai-Kitahata, Yasuhiro Asahina, Shinji Tanaka, Sei Kakinuma, Miyako Murakawa, Sayuri Nitta,
Takako Watanabe, Satoshi Otani, Miki Taniguchi, Fumio Goto, Hiroko Nagata, Shun Kaneko, Megumi
Tasaka-Fujita, Yuki Nishimura-Sakurai, Seishin Azuma, Yasuhiro Itsui, Mina Nakagawa, Minoru Tanabe,
Shinichi Takano, Mitsuharu Fukasawa, Minoru Sakamoto, Shinya Maekawa, Nobuyuki Enomoto, Mamoru
Watanabe. Comprehensive analyses of mutations and hepatitis B virus integration in hepatocellular
carcinoma with clinicopathological features. J. Gastroenterol. 2016.03; 51(5); 473-486
6. Kawai-Kitahata F, Asahina Y, Tanaka S, Kakinuma S, Murakawa M, Nitta S, Watanabe T, Otani S,
Taniguchi M, Goto F, Nagata H, Kaneko S, Tasaka-Fujita M, Nishimura-Sakurai Y, Azuma S, Itsui Y,
Nakagawa M, Tanabe M, Takano S, Fukasawa M, Sakamoto M, Maekawa S, Enomoto N, Watanabe M..
Comprehensive analyses of mutations and hepatitis B virus integration in hepatocellular carcinoma withclinicopathological features. J Gastroenterol. . 2016.05; 51(5); 473-486
7. Iwasa Y, Kitazume Y, Tateishi U, Saida Y, Ban D, Tanabe M, Takemoto A.. Hepatocellular Carcinoma
Histological Grade Prediction: A Quantitative Comparison of Diﬀusion-Weighted, T2-Weighted, andHepatobiliary-Phase Magnetic Resonance Imaging. J Comput Assist Tomogr. . 2016.05; 40(3); 463470
8. Eriko Katsuta, Atsushi Kudo, Takumi Akashi, Yusuke Mitsunori, Satoshi Matsumura, Arihiro Aihara,
Daisuke Ban, Takanori Ochiai, Shinji Tanaka, Yoshinobu Eishi, Minoru Tanabe. Macroscopic morphology
for estimation of malignant potential in pancreatic neuroendocrine neoplasm J Cancer Res Clin Oncol .
2016.06; 142; 1299-1306
9. Yamada Y, Hoshino K, Irie R, Tomita H, Kato M, Shimojima N, Fujino A, Hibi T, Shinoda M, Obara H,
Itano O, Kawachi S, Tanabe M, Sakamoto M, Kitagawa Y, Kuroda T.. The optimal immunosuppressive
protocol for the portal vein infusion of PGE1 and methylprednisolone in pediatric liver transplantation
for fulminant hepatic failure of unknown etiology. Pediatr Transplant.. 2016.08; 20(5); 640-646
10. Oba A, Ban D, Kirimura S, Akahoshi K, Mitsunori Y, Matsumura S, Ochiai T, Kudo A, Tanaka S, Minoru
T.. Clinical application of the biomarkers for the selection of adjuvant chemotherapy in pancreatic ductal
adenocarcinoma. J Hepatobiliary Pancreat Sci. . 2016.08; 23(8); 480-488
11. Akahoshi K, Tanaka S, Mogushi K, Shimada S, Matsumura S, Akiyama Y, Aihara A, Mitsunori Y, Ban
D, Ochiai T, Kudo A, Arii S, Tanabe M.. Expression of connective tissue growth factor in the livers of
non-viral hepatocellular carcinoma patients with metabolic risk factors. J Gastroenterol. . 2016.09; 51(9);910-922
12. Yuichiro Watanabe, Shinya Honda, Akimitsu Konishi, Satoko Arakawa, Michiko Murohashi, Hirofumi
Yamaguchi, Satoru Torii, Minoru Tanabe, Shinji Tanaka, Eiji Warabi, Shigeomi Shimizu. Autophagy
controls centrosome number by degrading Cep63. Nat Commun. 2016.11; 7; 13508
13. Furuyama T, Kudo A, Matsumura S, Mitsunori Y, Aihara A, Ban D, Ochiai T, Tanaka S, Tanabe M.. Preoperative direct bilirubin to prothrombin time ratio index to prevent liver failure after minor hepatectomy.
J Hepatobiliary Pancreat Sci. . 2016.12; 23(12); 763-770
14. Norihiro Kishida, Masahiro Shinoda, Osamu Itano, Hideaki Obara, Minoru Kitago, Taizo Hibi, Hiroshi
Yagi, Yuta Abe, Kentaro Matsubara, Masanori Odaira, Minoru Tanabe, Motohide Shimazu, Yuko Kitagawa. Increased Incidence of Thrombotic Microangiopathy After ABO-Incompatible Living Donor Liver
Transplantation. Ann. Transplant.. 2016.12; 21; 755-764[Conference Activities & Talks]
1. Akahoshi K, Ochiai T, Ito H, Matsumura S, Mitsunori Y, Aihara A, Ban D, Kudo A, Tanaka S, Tanabe
M. Evaluation of POSSUM score for predicting morbidity after hepatectomy for hepatocellular carcinoma.
11th Annual Academic surgical Congress 2016.02.04 Florida
2. Itou H, Ochiai T, Matsumura S, Mitsunori Y, Aihara A, Ban D, Kudo A, Tanabe M. The consensus andcontroversial points of new IPMN guideline. 11th Annual Academic surgical Congress 2016.02.04 Florida― 408 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

3. Ochiai T, Miura T, Tanaka S, Yamazaki S, Kudo A. Noguchi N, Irie T, Ban D, Aihara A, Matsumura
S, Mitsunori Y, Ito H, Akahoshi K, Tanabe M. Is papillectomy for early ampullary carcinoma justiﬁed?
-Analysis of 65 resected cases - . 11th Annual Academic surgical Congress 2016.02.04 Florida
4. Akahoshi K, Kudo A, Tanabe M. Ischemic Reperfusion Injury by Selective Hepatic Artery Clampig Iduced
the Sinusoidal Flow Increase in Rat. Proceedings of the Second International Symposium 2016.02.11 Aichi
5. Tanabe M. State of the Art in radiofrequency, micro-wave, and cryoablation. ASIA
Course in Hepatobiliary and Pancreatic surgery 2016.02.26 Taiwan

IRCAD Advanced

6. Tanabe M. A diﬃculty scoring system for resection for hepatocellular carcinoma located in segments of
the liver. ASIA IRCAD Advanced Course in Hepatobiliary and Pancreatic surgery 2016.02.26 Taiwan
7. Tanabe M. Technical points: Anatomy around the SMA in pancreatic surgery. ASIA
Course in Hepatobiliary and Pancreatic surgery 2016.02.26 Taiwan

IRCAD Advanced

8. Kudo A, Tanaka S, Tanabe M. Parametric Imaging to estimate HCC malignancy. Ultrasonic Week 20162016.05.28 Kyoto
9. Ban D, Iwao Y, Matsumura S, Ono h, Aihara A, Mitsunori Y, Kudo A, Tanabe M. The optimal pancreatic
stump closure method of a laparoscopic distal pancreatectomy: wrapping and ﬁbrin capping technique..
Second Triangle Symposium of the Poland-Hungary-Japan Surgical Society 2016.06.20 Lublin, Poland
10. Oba A, Ban D, Kirimura S, Akahoshi K, Kudo A, Ito H, Matsumura S, Mitsunori Y, Aihara A, Ochiai
T, Tanaka S, Tanabe M. Impact of the Biomarkers for the Selection of Gemcitabine and S-1 Adjuvant
Chemotherapy in Pancreatic Ductal Adenocarcinoma. IAP&JPS&AOPA2016 2016.08.04 Sendai
11. Ban D, Iwao Y, Ishikawa Y, Watanabe S, Mizuno Y, Chiyonobu N, Ueda H, Ohata Y, Sato T, Mitsunori
Y, Matsumura S, Aihara A, Ochiai T, Kudo A, Tanaka S, Tanabe M. The role of local treatment for therecurrence of pancreatic cancer after pancreatectomy. IAP&JPS&AOPA2016 2016.08.04 Sendai
12. Kudo A, Tanaka S, Tanabe M. Hormonal map determines the indication of regional therapy for patientswith functional pancreatic neuroendocrine tumor. IAP&JPS&AOPA2016 2016.08.05 Sendai
13. Iwao Y, Ban D, Ishikawa Y, Watanabe S, Ishikawa Y, Mizuno Y, Chiyonobu N, Ueda H, Ohata Y, Oba A,
Sato T, Akahoshi K, Ito H, Mitsunori Y, Matsumura S, Aihara A, Ochiai T, Kudo A, Tanaka S, Tanabe
M. analysis of the image ﬁndings around superior mesenteric artery of invasive ductal carcinoma in thepancreas head. IAP&JPS&AOPA2016 2016.08.06 Sendai
14. Tanabe M. Necessity of a systematic preoperative imaging before laparoscopic cholecystomy ? -Eastern
experience. Ircad France 2016 2016.08.21 Strasbourg
15. Tanabe M. Controversies – One port cholesystectomy ; pro side. Ircad France 2016 2016.08.22 Strasbourg
16. Tanabe M. SILS Laparoscopic cholecystectomy Prerecorded LIVE case : Single Port Laparoscopic cholecystectomy. Ircad France 2016 2016.08.22 Strasbourg
17. Tanabe M. Experimental Laboratory - Practice on Live Tissue. Ircad France 2016 2016.08.24 Strasbourg
18. Iwao Y, Ojima H, Tanabe M. Mechanism of Liver Atrophy due to Portal vein Embolization - Association
with Autophagy and Apoptosis - . UEGweek 2016 2016.10.17 Vienna,Austria
19. Sato T, Ochiai T, Iwao Y, Ono H, Mitsunori Y, Matsumura S, Aihara A, Ban D, Kudo A, Tanaka S,
Tanabe. Prognosis and Therapy for Ruptured Hepatocellular Carcinoma: A single Hospital Experiences.
40th World Congress of the International College of Surgeons 2016.10.25 Kyoto
20. Ochiai T, Yoshino J, Asano D, Watanabe S, Ishikawa Y, Mizuno Y, Chiyonobu N, Ueda H, Sato T, Iwao
Y, Ono H, Mitsunori Y, Matsumura S, Aihara A, Ban D, Kudo A, Tanaka S, Tanabe M. The impact
of Neo-adjuvant and Adjuvant Hepatic Arterial Infusion Chemotherapy Combined with Hepatectomy for
Patients with Advanced Hepatocellular Carcinoma. 40th World Congress of the International College ofSurgeons 2016.10.25 Kyoto
21. Ban D. Intraoperative Bleeding Control. WCES & ELSA & CSLES 2016.11.10 Shanghai
22. Tanabe M. Special Lectures. Japan-Taiwan Medical Spectroscopy International Symposium（JTMSIS)
2016.12.07 Awaji Island,Japan― 409 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences[Patents]
1. ORGAN FUNCTION MAINTAINING AND AMELIORATING SOLUTION, Patent Number：PCT/JP2006/304269[Awards & Honors]
1. the Basis Science Travel Grant award of the United European Gastroenterology Week 2016, United European Gastroenterology(UEG), 2016.10― 410 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

Orthopaedic and Spinal Surgery

Professor: Atsushi Okawa
Associate Professor: Toshitaka Yoshii
Assistant Professor: Hiroyuki Inose, Yuko Segawa, Koji Fujita, Takashi Hirai, Yoto Oh, Tsuyoshi Yamada
Department of Orthopaedic Research and Development
Associate Professor: Shinichi Sotome, Yoshinori Asou
Joint Research Department of Advanced Medical Technology
Specially Appointed Professor: Shigenori Kawabata(1)

OutlineMembers of our section and Orthopaedic Joint Sugery section work together in a clinic and OR. Throughthese practices we train to make the clinical diagnosis and to plan the adequate surgery. We study ﬁndings ofclinical problem of the locomotorium lesion such as joints, spine and spinal cord, peripheral nerve disorders,aging, injury, tumorigenesis mechanism, and image ﬁndings. To solve a lot of clinical question and develop new
methodology to treat patients having severe orthopaedic problems, we especcially research spinal cord function,bone regeneration, and pain perception mechanism at dorsal root ganglion.

(2)

ResearchResearch themes:
Bone and cartilage metabolism
Development and evaluation of biomaterials for clinical application
Mechanism of spinal ligament ossiﬁcation
Development of measuring device for spinal cord magnetic signals
Research of bone and spinal metastatic tumors
We collaborate with other sections in our university such as the Clinical Anatomy, the Neurology, and the
Physiology and Cell Biology.

(3)

Education

Our department has several regular program such as "Bedside Professor Round" at Monday 14:30-16:30, "ClinicalConference" at Monday 7:30-9:00, and "Jounal Clubb or Research Progress meeting" at 7:30-8:00 of Tuesday,Thursday, and Friday.
Graduate students in our department can acquire the basic techmiques of orthpaedic research and can learn a
up-dated knowledge of clinical medicaine through regularly-held journal clubs and research meetings.

― 411 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced(4)

Lectures & Courses

Japanese orthpaedic research is charactorised by the fact that orthopaedc surgeon himself participates experiments while he is working as a clinician. A lot of new knowledge concerning bone, cartilage and nerve werediscovered by this so-called "surgeon scientist".
We have already taken a new artiﬁcial bone developed in our section to the market and have been preparing
a revolutional measurering device for spinal magnetic signals. We think it very important that research by asurgeon should be based on clinical problems even when methodoloy of molecular biology is used.
Our graduate students learn basic technique of orthopaedic research and also acquire the ability of life-cotinueing
attitude for clinical studies.
(5)

Clinical Services & Other Works

Our orthopaedic department consists of two graduate school sectiones, the Orthopaedic and Spinal Surgery and
the Joint Surgery and Sports Medicine. We deal wtih all kinds of orthopaedic diseases such as spine, hand, hip,knee,and musclo-skeletal tumor. More than twenty registerd orthopaedic surgeons belong to our department.
Our anterior cervical operation fot OPLL results in a good clinical outcome. We also organize many spinal
surgeons who are members of a nation-wide research organization for spinal ligament ossiﬁcation supported by
the Ministry of Health, Labour and Welfare.
(6)

Clinical Performances

We aim to provide safer surgery to the patinets with intractable spinal disease using many kinds of modality
as navigation, microscopic surgery, spinal cord montoring, and intraoperative CAT scan. Treatments of adultspinal defromity and osteopotic vertebral fracture are our other interest. We have also developed an originalartiﬁcial bone composed of hydroxy-appatite and collagen, now promoting to use aggressively to ﬁll large bone
defect.
(7)

Publications[Original Articles]
1. Yoshinori Asou, Munetaka Iwata, Hiroki Ochi, Maierhaba Ailixiding, Zulipiya Aibibula, Jinying Piao,
Guangwen Jin, Yasushi Hara, Atsushi Okawa. Pleiotropic Functions of High Fat Diet in the Etiology of
Osteoarthritis. PLoS ONE. 2016; 11(9); e0162794
2. Takashi Hirai, Toshitaka Yoshii, Akio Iwanami, Kazuhiro Takeuchi, Kanji Mori, Tsuyoshi Yamada,
Kanichiro Wada, Masao Koda, Yukihiro Matsuyama, Katsushi Takeshita, Masahiko Abematsu, Hirotaka Haro, Masahiko Watanabe, Kei Watanabe, Hiroshi Ozawa, Haruo Kanno, Shiro Imagama, Shunsuke Fujibayashi, Masashi Yamazaki, Morio Matsumoto, Masaya Nakamura, Atsushi Okawa, Yoshiharu
Kawaguchi. Prevalence and Distribution of Ossiﬁed Lesions in the Whole Spine of Patients with CervicalOssiﬁcation of the Posterior Longitudinal Ligament A Multicenter Study (JOSL CT study). PLoS ONE.
2016; 11(8); e0160117
3. Tomokazu Masaoka, Toshitaka Yoshii, Masato Yuasa, Tsuyoshi Yamada, Takashi Taniyama, Ichiro Torigoe, Kenichi Shinomiya, Atsushi Okawa, Sadao Morita, Shinichi Sotome. Bone Defect Regeneration by
a Combination of a β-Tricalcium Phosphate Scaﬀold and Bone Marrow Stromal Cells in a Non-Human
Primate Model. Open Biomed Eng J. 2016; 10; 2-11
4. Takada R, Jinno T, Tsutsumi Y, Doi H, Muneta T, Okawa A. Inhibitory Eﬀect of Zirconium Coating toBone Bonding of Titanium Implants in Rat Femur Mater Trans. 2016;
5. Tsuyoshi Yamada, Toshitaka Yoshii, Hiroaki Yasuda, Atsushi Okawa, and Shinichi Sotome. Dexamethasone Regulates EphA5, a Potential Inhibitory Factor with Osteogenic Capability of Human Bone Marrow
Stromal Cells Stem Cells International. 2016.01; 2016;
6. Yamada T, Yoshii T, Egawa S, Takada R, Hirai T, Inose H, Kato T, Jinno T, Okawa A.. Drain Tip Culture
is Not Prognostic for Surgical Site Infection in Spinal Surgery Under Prophylactic Use of Antibiotics. Spine(Phila Pa 1976). 2016.02;― 412 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

7. Sakie Katsumura, Yoichi Ezura, Yayoi Izu, Jumpei Shirakawa, Atsushi Miyawaki, Kiyoshi Harada, Masaki
Noda. Beta Adrenergic Receptor Stimulation Suppresses Cell Migration in Association with Cell Cycle
Transition in Osteoblasts-Live Imaging Analyses Based on FUCCI System. J. Cell. Physiol.. 2016.02;
231(2); 496-504
8. Yusuke Nakagawa, Ichiro Sekiya, Shimpei Kondo, Takashi Tabuchi, Shizuko Ichinose, Hideyuki Koga,
Kunikazu Tsuji, Takeshi Muneta. Relationship between MRI T1 rho value and histological ﬁndings ofintact and radially incised menisci in microminipigs. J Magn Reson Imaging. 2016.02; 43(2); 434-445
9. Cheng Xu, Hiroki Ochi, Toru Fukuda, Shingo Sato, Satoko Sunamura, Takeshi Takarada, Eiichi Hinoi,
Atsushi Okawa, Shu Takeda. Circadian clock regulates bone resorption in mice J. Bone Miner. Res..
2016.02; 31(7); 1344-1355
10. Shimpei Kondo, Takeshi Muneta, Yusuke Nakagawa, Hideyuki Koga, Toshifumi Watanabe, Kunikazu
Tsuji, Shinichi Sotome, Atsushi Okawa, Shinji Kiuchi, Hideo Ono, Mitsuru Mizuno, Ichiro Sekiya. Transplantation of autologous synovial mesenchymal stem cells promotes meniscus regeneration in aged primates. J. Orthop. Res.. 2016.02;
11. Masanori Saito, Mieradili Mulati, S Zakiah A Talib, Philipp Kaldis, Shu Takeda, Atsushi Okawa, Hiroyuki
Inose. The Indispensable Role of Cyclin-Dependent Kinase 1 in Skeletal Development. Sci Rep. 2016.02;
6; 20622
12. Lin W, Ezura Y, Izu Y, Aryal SA, Kawasaki M, Chantida PN, Moriyama K, Noda M. Proﬁlin Expression Is
Regulated by Bone Morphogenetic Protein (BMP) in Osteoblastic Cells. Journal of Cellular Biochemistry.
2016.03; 117(3); 621-628
13. Shinichi Sotome, Keisuke Ae, Atsushi Okawa, Masafumi Ishizuki, Hideo Morioka, Seiichi Matsumoto,Takashi Nakamura, Satoshi Abe, Yasuo Beppu, Kenichi Shinomiya. Eﬃcacy and safety of porous hydroxyapatite/type 1 collagen composite implantation for bone regeneration: A randomized controlled study.
J Orthop Sci. 2016.03;
14. Tomokazu Masaoka, Tsuyoshi Yamada, Masato Yuasa, Toshitaka Yoshii, Atsushi Okawa, Sadao Morita,
Yuko Kozaka, Masahiro Hirano, Shinichi Sotome. Biomechanical evaluation of the rabbit tibia after
implantation of porous hydroxyapatite/collagen in a rabbit model. J Orthop Sci. 2016.03; 21(2); 230-236
15. Zulipiya Aibibula, Maierhaba Ailixiding, Munetaka Iwata, Jinying Piao, Yasushi Hara, Atsushi Okawa,
Yoshinori Asou. Xanthine oxidoreductase activation is implicated in the onset of metabolic arthritis.
Biochem. Biophys. Res. Commun.. 2016.03; 472(1); 26-32
16. Yamada T, Yoshii T, Hirai T, Inose H, Kato T, Kawabata S, Okawa A.. Clinical Outcomes of Spinal
Surgery for Patients Undergoing Hemodialysis. Orthopedics. 2016.05;
17. Zenya Ito, Yukihiro Matsuyama, Muneharu Ando, Shigenori Kawabata, Tsukasa Kanchiku, Kazunobu
Kida, Yasushi Fujiwara, Kei Yamada, Naoya Yamamoto, Sho Kobayashi, Takanori Saito, Kanichiro Wada,
Kazuhiko Satomi, Kenichi Shinomiya, Toshikazu Tani. What Is the Best Multimodality Combination for
Intraoperative Spinal Cord Monitoring of Motor Function? A Multicenter Study by the Monitoring
Committee of the Japanese Society for Spine Surgery and Related Research. Global Spine J. 2016.05;
6(3); 234-241
18. Nobutake Ozeki, Takeshi Muneta, Hideyuki Koga, Yusuke Nakagawa, Mitsuru Mizuno, Kunikazu Tsuji,
Y Mabuchi, C Akazawa, E Kobayashi, K Matsumoto, K Futamura, T Saito, Ichiro Sekiya. Not single
but periodic injections of synovial mesenchymal stem cells maintain viable cells in knees and inhibit
osteoarthritis progression in rats. Osteoarthr. Cartil.. 2016.06; 24(6); 1061-1070
19. Toshitaka Yoshii, Takashi Hirai, Kenichiro Sakai, Hiroyuki Inose, Tsuyoshi Kato, Atsushi Okawa. Cervical
pedicle screw placement using intraoperative computed tomography imaging with a mobile scanner gantry.
Eur Spine J. 2016.06; 25(6); 1690-1697
20. Kensuke Sekihara, Yuya Kawabata, Shuta Ushio, Satoshi Sumiya, Shigenori Kawabata, Yoshiaki Adachi,
Srikantan S Nagarajan. Dual signal subspace projection (DSSP): a novel algorithm for removing large
interference in biomagnetic measurements. J Neural Eng. 2016.06; 13(3); 036007― 413 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced

21. Suzuki H, Ito Y, Shinohara M, Yamashita S, Ichinose S, Kishida A, Oyaizu T, Kayama T, Nakamichi
R, Koda N, Yagishita K, Lotz MK, Okawa A, Asahara H.. Gene targeting of the transcription factor
Mohawk in rats causes heterotopic ossiﬁcation of Achilles tendon via failed tenogenesis. Proceedings of
the National Academy of Sciences of the United States of America. 2016.07; 113(28); 7840-7845
22. Sato S, Tang YJ, Wei Q, Hirata M, Weng A, Han I, Okawa A, Takeda S, Whetstone H, Nadesan P,Kirsch
DG, Wunder JS, Alman BA. Mesenchymal tumors can derive from Ng2/Cspg4-expressing pericytes with
β-catenin modulating the neoplastic phenotype Cell Reports. 2016.07; 16(4); 917-927
23. Atsushi Kimura, Atsushi Seichi, Katsushi Takeshita, Hirokazu Inoue, Tsuyoshi Kato, Toshitaka Yoshii,
Takeo Furuya, Masao Koda, Kazuhiro Takeuchi, Shunji Matsunaga, Shoji Seki, Yoshimoto Ishikawa, Shiro
Imagama, Masashi Yamazaki, Kanji Mori, Yosuke Kawasaki, Koji Fujita, Kenji Endo, Kimiaki Sato,
Atsushi Okawa. Fall-related Deterioration of Subjective Symptoms in Patients with Cervical Myelopathy.
Spine. 2016.07;
24. Tsuyoshi Yamada, Toshitaka Yoshii, Satoru Egawa, Ryohei Takada, Takashi Hirai, Hiroyuki Inose, Tsuyoshi
Kato, Tetsuya Jinno, Atsushi Okawa. Drain Tip Culture is Not Prognostic for Surgical Site Infection in
Spinal Surgery Under Prophylactic Use of Antibiotics. Spine. 2016.07; 41(14); 1179-1184
25. Haruhiko Shimura, Akimoto Nimura, Hisayo Nasu, Hitomi Fujishiro, Junya Imatani, Atsushi Okawa,Keiichi Akita. Joint capsule attachment to the coronoid process of the ulna: an anatomic study with
implications regarding the type 1 fractures of the coronoid process of the O’Driscoll classiﬁcation. J
Shoulder Elbow Surg. 2016.09; 25(9); 1517-1522
26. Zenya Ito, Yukihiro Matsuyama, Muneharu Ando, Shigenori Kawabata, Tsukasa Kanchiku, Kazunobu
Kida, Yasushi Fujiwara, Kei Yamada, Naoya Yamamoto, Sho Kobayashi, Takanori Saito, Kanichiro Wada,
Nobuaki Tadokoro, Masato Takahashi, Kazuhiko Satomi, Kenichi Shinomiya, Toshikazu Tani. Postoperative Paralysis From Thoracic Ossiﬁcation of Posterior Longitudinal Ligament Surgery Risk Factor of
Neurologic Injury: Nationwide Multiinstitution Survey. Spine. 2016.10; 41(19); E1159-E1163
27. R Takada, T Jinno, D Koga, M Hirao, T Muneta, A Okawa. Comparison of wear rate and osteolysis
between annealed and remelted highly cross-linked polyethylene in total hip arthroplasty. A case control
study at 7 to 10 years follow-up. Orthop Traumatol Surg Res. 2016.10; 102(6); 717-721
28. Toshitaka Yoshii, Kenichiro Sakai, Takashi Hirai, Tsuyoshi Yamada, Hiroyuki Inose, Tsuyoshi Kato,
Mitsuhiro Enomoto, Shoji Tomizawa, Shigenori Kawabata, Yoshiyasu Arai, Atsushi Okawa. Anterior
decompressionwithfusionversusposteriordecompressionwithfusionformassivecervicalossiﬁcationofthe
posteriorlongitudinalligamentwitha≥ 50%canaloccupyingratio:amulticenterretrospectivestudy.SpineJ.2016.11;16(11);1351-1357
29. Hiroyuki Inose, Tsuyoshi Yamada, Mieradili Mulati, Takashi Hirai, Shuta Ushio, Toshitaka Yoshii, Tsuyoshi
Kato, Shigenori Kawabata, Atsushi Okawa. Bone Turnover Markers as a New Predicting Factor for Nonunion After Spinal Fusion Surgery. Spine. 2016.11;
30. Naofumi Taniguchi, Tetsuya Jinno, Daisuke Koga, Tetsuo Hagino, Atsushi Okawa, Hirotaka Haro. Cementless Hip Stem Anteversion in the Dysplastic Hip: A Comparison of Tapered Wedge vs Metaphyseal
Filling. J Arthroplasty. 2016.12;
31. Kanji Mori, Toshitaka Yoshii, Takashi Hirai, Akio Iwanami, Kazuhiro Takeuchi, Tsuyoshi Yamada, ShojiSeki, Takashi Tsuji, Kanehiro Fujiyoshi, Mitsuru Furukawa, Soraya Nishimura, Kanichiro Wada, Masao
Koda, Takeo Furuya, Yukihiro Matsuyama, Tomohiko Hasegawa, Katsushi Takeshita, Atsushi Kimura,
Masahiko Abematsu, Hirotaka Haro, Tetsuro Ohba, Masahiko Watanabe, Hiroyuki Katoh, Kei Watanabe,
Hiroshi Ozawa, Haruo Kanno, Shiro Imagama, Zenya Ito, Shunsuke Fujibayashi, Masashi Yamazaki,
Morio Matsumoto, Masaya Nakamura, Atsushi Okawa, Yoshiharu Kawaguchi. Prevalence and distributionof ossiﬁcation of the supra/interspinous ligaments in symptomatic patients with cervical ossiﬁcation of
the posterior longitudinal ligament of the spine: a CT-based multicenter cross-sectional study. BMC
Musculoskelet Disord. 2016.12; 17(1); 492[Conference Activities & Talks]
1. Tsuyoshi Yamada, Hideyuki Iwai, Sayuri Nitta, Masakazu Nagahori , Masayoshi Harigai . Creating Medical Innovators – a Whole-brain Approach to Healthcare. 13TH ASIA PACIFIC MEDICAL EDUCATIONCONFERENCE (APMEC) 2016 2016.01.15― 414 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

2. Toshiyuki Ohara,Takeshi Muneta, Yusuke Nakagawa, Yu Matsukura, Kunikazu Tsuji, Ichiro Sekiya. Hypoxia enhances proliferation with chondrogenic potential in primary synovial MSCs. Orthopaedic ResearchSociety 2016 Annual Meeting 2016.03.05
3. Yusuke Nakagawa, Hideyuki Koga, Takeshi Muneta, Kiuchi S, Ono H, Nagata T, Toshifumi Watanabe,
Masafumi Horie, Tomomasa Nakamura, Koji Otabe, Mai Katakura, Hisako Katano, Ichiro Sekiya. Threedimensional Reconstructed MRI Evaluation of Arthroscopic Centralization Technique for Extruded Lateral
Meniscus. Orthopaedic Research Society 2016 Annual Meeting 2016.03.05
4. Toshifumi Watanabe,Takeshi Muneta, Ichiro Sekiya, Hideyuki Koga, Masafumi Horie, Tomomasa Nakamura, Koji Otabe, Scott Banks. In-vivo Kinematics of Posterior-stabilized Total Knee Prosthesis Designed
for Asian. Orthopaedic Research Society 2016 Annual Meeting 2016.03.05
5. Gaku Koyano, Tetsuya Jinno, Daisuke Koga, Takeshi Muneta, Atsushi Okawa. Bilateral Comparison of
Hip Joint Morphology between the Hips with and without Herniation Pit. Orthopaedic Research Society
2016 Annual Meeting 2016.03.05 Orlando, USA
6. Hidetsugu Suzuki, Yoshiaki Ito, Masahiro Shinohara, Ryo Nakamichi, Shizuko Ichinose, Atsushi Okawa,
Hiroshi Asahara. Mohawk Knock Out Rats Revealed Early Heterotopic Ossiﬁcation Of Achilles Tendon
Through Failed Tenogenesis . Orthopaedic Research Society 2016 Annual Meeting 2016.03.05 Orlando,USA
7. T. Hirai, T Yoshii, A Iwanami, K Takeuchi, K Mori, A Okawa, Y Kawaguchi.. Co-existence of Ossiﬁcationof the Nuchal Ligament is Associated with Hyperostosis in the Whole Spine in Patients with CervicalOssiﬁcation of the Posterior Longitudinal Ligament . CSRS-AP2016 2016.04.21 seoul, Korea
8. T. Hirai, T Yoshii, A Iwanami, K Takeuchi, K Mori, T Yamada, S Nishimura, M Matsumoto, M Nakamura,A Okawa, Y Kawaguchi.. Incidence and Distribution of Ossiﬁed Lesions in the Whole Spine of Patientswith Cervical Ossiﬁcation of the Posterior Longitudinal Ligament – A Multicenter Study . CSRS-AP2016
2016.04.21 seoul, Korea
9. Tsuyoshi Kato , Toshitaka Yoshii , Hiroyuki Inose , Takashi Hirai, Tsuyoshi Yamada, Atsushi Okawa. Prevertebroplasty transpedicular vertebrography assessment in delayed-union/pseudarthrosis associatedwith osteoporotic vertebral fracture . ISSLS 2016 Singapore 2016.05.17 Singapore
10. Mari Uomizu, Hideya Yoshimura, Akimoto Nimura, Takashi Miyamoto, Tomoyuki Mochizuki. Return toSports Activity after Arthroscopic Rotator Cuﬀ Repair on the Middle-aged and Senior Patients. The 13th
International Congress of Shoulder and Elbow Surgery 2016.05.18 Korea
11. Atsuhiro Tano, Yoto Oh, Yoshiro Kurosa, Atsushi Okawa . Tangential view for percutaneous screwﬁxation of pelvic ring fractures: preoperative calibration of computed tomographic image . 3rd AOTAP
Scientiﬁc Congress & TK Experts’ Symposium 2016.05.27 China
12. Yoto Oh , Yoshiaki Wakabayashi , Yoshiro Kurosa , Koji Fujita , Atsushi Okawa . Pathogenic mechanism
and treatment precaution for atypical femoral fractures: subtype classiﬁcation through CT-based ﬁnite
element analysis. 3rd AOTAP Scientiﬁc Congress & TK Experts’ Symposium 2016.05.27 China
13. Ryohei Takada, Tetsuya Jinno, Daisuke Koga, Masanobu Hirao, Takeshi Muneta, Atsushi Okawa. Limited
signiﬁcance of screening computed tomography after cementless total hip arthroplasty with highly crosslinked polyethylene.. 17th EFORT 2016.06.01 Geneva,Schwzerland
14. Ryohei Takada, Tetsuya Jinno, Daisuke Koga, Masanobu Hirao, Takeshi Muneta, Atsushi Okawa. Comparison of wear rate and osteolysis between annealed and remelted highly cross-linked polyethylene intotal hip arthroplasty.. 17th EFORT 2016.06.01 Geneva,Schwzerland
15. Oyaizu T, Enomoto M, Yagishita K.. Hyperbaric and high-oxygen environments lead to macrophageinﬁltration, stimulate cell proliferation and accelerate muscle regeneration in contused rat skeletal muscle..
Undersea and Hyperbaric Medical Society Annual Scientiﬁc Meeting 2016 2016.06.09 Las Vegas, UnitedStates of America
16. Clinical Outcomes of Lumbar Surgery for Hemodialysis Patients. 2016.09.02
17. Hidetoshi Kaburagi, Koji Fujita, Takashi Miyamoto, Yoshiaki Wakabayashi, Ryuichi Kato, Atsushi Okawa.
Distal radius fracture patients show reduced ability of dynamic body balancing. The American Society
for Bone and Mineral Research 2016 Annual Meeting 2016.09.16 Atlanta, USA― 415 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced

18. Koji Fujita, Hidetoshi Kaburagi, Yoshiaki Wakabayashi, Ryuichi Kato, Atsushi Okawa. Distal Radius
Fracture Patients Show Declined Ability of Dynamic Body Balancing. 71st Annual Meeting of the American Society for Surgery of the Hand（ASSH 2016） 2016.09.29 Austin,USA
19. Koji Fujita, Kenji Kimori, Yutaka Kadonishi, Hiroki Yamanaka, Yoshikazu Ikuta. MRI analysis of Carpal
tunnel syndrome in hemodialysis patients, compared to non-hemodialysis patients.. 71st Annual Meetingof the American Society for Surgery of the Hand（ASSH 2016） 2016.09.29 Austin,USA
20. Yuki Hasegawa, Kensuke Sekihara, Yasuhiro Shirai, Taishi Watanabe, Yoshiaki Adachi, Kenzo Hirao,
Shigenori Kawabata. . Comparison between Hosaka-Cohen transformation and 2D source image in MCG
study .. BIOMAG2016(20th International Conference on Biomagnetism) 2016.10.01 Seoul, Korea
21. Yasuhiro Shirai,Kenzo Hirao, Taishi Watanabe, Shuta Ushio, Shigenori Kawabata. . Visualization of
the electrical current in atrioventricular node by magnetocardiograms.. BIOMAG2016(20th International
Conference on Biomagnetism) 2016.10.01 Seoul, Korea
22. Yasuhiro Shirai,Kenzo Hirao, Tomohiro Shibuya, Shuta Ushio, Shigenori Kawabata. . Mesurement ofmagnetocardiogram using magnetoresistive sensor.. BIOMAG2016(20th International Conference on Biomagnetism) 2016.10.01 Seoul, Korea
23. Shigenori Kawabata, Shuta Ushio, Tomohiko Shibuya, Yoshiaki Adachi, Kensuke Sekihara, Atsushi Okawa.
. Measurement of magnetomyography using an array of magnetoresistive ( MR) sensor.. BIOMAG2016(20thInternational Conference on Biomagnetism) 2016.10.01 Seoul, Korea
24. Shigenori Kawabata, Satoshi Sumiya, Yuko Hoshino, Yoshiaki Adachi, Kensuke Sekihara, Shuta Ushio,
Taishi Watanabe, Atsushi Okawa. . Visualization of electrophysiological activity in cervical spinal cordand spinal nerves by magnetospinography.. BIOMAG2016(20th International Conference on Biomagnetism) 2016.10.01 Seoul, Korea
25. Ushio S, Kawabata S, Sumiya S, Ukegawa D, Sekihara K, Adachi Y, Okawa A. . Non-invasive fuctional
evaluation of lumbar nerveroot and cauda equina with high spatial re-solution by magnetospinographysystem.. BIOMAG2016(20th International Conference on Biomagnetism) 2016.10.01 Seoul, Korea
26. Koji Fujita, Kenji Kimori, Yutaka Kadonishi, Hiroki Yamanaka, Yoshikazu Ikuta. MRI analysis of Carpal
tunnel syndrome in hemodialysis patients, compared to non-hemodialysis patients. International Federation of Societies for Surgery of the Hand 2016 2016.10.24 Buenos Aires, Argentina
27. Koji Fujita, Hidetoshi Kaburagi, Yoshiaki Wakabayashi, Atsushi Okawa. Distal radius fracture patientsshow reduced ability of dynamic body balancing. International Federation of Societies for Surgery of theHand 2016 2016.10.24 Buenos Aires, Argentina
28. Shingo Sato, Yuning J. Tang, Qingxia Wei, Makoto Hirata, Ilkyu Han, Shu Takeda, David G. Kirsch, Jay
S. Wunder, Benjamin A. Alman.. Mesenchymal tumors can derive from NG2-expressing pericytes withbeta-catenin modulating the neoplastic phenotype.. Connective Tissue Oncology Society, the 21st Annual
Meeting 2016.11.09 Lisbon. Portugal
29. Yusuke Minami、Seiichi Matsumoto、Keisuke Ae、Taisuke Tanizawa、Tabu Gokita、Keiko Hayakawa、Yuki Funauchi、Munehisa Kito、Yusuke Sumi、Yoshiya Sugiura. Retrospective study between FNCLCC
grade 2 and grade 3 cases in resectable synovial sarcomas. Connective Tissue Oncology Society 2016
（CTOS2016） 2016.11.09 Lisbon,Portugal
30. Yusuke Minami、Eisuke Kobayashi、Daisuke Kubota、Nokitaka Setsu、Yoshikazu Tanzawa、Fumihiko
Nakatani、Akira Kawai、Hirokazu Chuman. An unusual local invasion of myxoid liposarcoma. Connective
Tissue Oncology Society 2016（CTOS2016） 2016.11.09 Lisbon,Portugal
31. Hidetoshi Kaburagi, Takashi Hirai, Takanori Yokota, Atsushi Okawa, Mitsuhiro Enomoto. Role of voltagegated sodium channels and calcium channel subunit alpha-2/delta-1 in the lumbar dorsal root ganglions
of persistent peripheral neuropathic pain model mice . Society for Neuroscience 2016Annual Meeting2016.11.12 San Diego,USA
32. T Hirai, T Yoshii, S Egawa, K Shinomiya, A Okawa.. Long-Term Results Of A Prospective Comparative
Study Of Anterior Decompression With Fusion (Adf) And Posterior Decompression With Laminoplasty
For The Treatment Of Cervical Spondylotic Myelopathy . 2016 CSRS 2016.11.30 Toronto,Canada― 416 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

33. T Yoshii, T Hirai, K Sakai, A Okawa, K Shinomiya.. A Prospective Comparative Study in Skin Antiseptic
Solutions for Posterior Spine Surgeries: Chlorhexidine-Gluconate Ethanol Vs. Povidone-Iodine . 2016
CSRS 2016.11.30 Toronto,Canada― 417 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

Diagnostic Radiology and Nuclear Medicine

Professor Ukihide Tateishi
Project Professor Yukihisa Saida
Associate Professors Ichiro Yamada
Lecturers Mitsuhiro Kishino(∼Mar),Yoshio Kitazume,
Yoshiaki Katada(Apr∼)
Research Associates Yoshiaki Katada(∼Mar),
Makiko Honda,Tomohiro Yoneyama(Apr∼),Akira Troriihara,
Kaori Okazawa(∼May),Masashi Nakadate(∼May),
Tomoyuki Fujioka, Yoshihiro Iwasa(June∼),Jun Oyama(June∼)Hospital Staﬀ members Yoshihiro Iwasa(∼May),Yoko Shirakawa,
Namiko Matsui(∼Mar),Ken Yamagiwa(Apr∼Sep),
Sayumi Michishige(Oct∼),Akane Ozawa(Oct∼),Kyoko Sugimoto(Oct∼)
Resident Jun Oyama(∼Mar),Emi Yamaga(∼Mar),Ken Yamagiwa(∼Mar),Takuya Adachi(Apr∼),Akane Ozawa(Apr∼Sep),
Kyoko Sugimoto(Apr∼Sep),Nobuhiro Takaya(Apr∼),Kenichi Fukui(Apr∼),Ruonan Li(Apr∼)
Graduate Students Junichi Tsuchiya,Minobu Kamo,
Yusuke Ogihara,Wataru Yamashita,Emi Yamaga,Ryo Miyazawa(1)

OutlineWhile diagnostic radiology and nuclear medicine demand high-level capabilities and therefore extensive training to acquire those capabilities, keeping a watch on developments in medical knowledge and maintaining thoseskills is also an issue for these disciplines. Similarly, it is also necessary to maintain knowledge, skills andcapabilities in ethics, not only radiology knowledge and skills, in order to respond to changes in medical practices as well as the social and political environment. Contributing to the community is a basic responsibilityof diagnostic radiology so it remains the university s mission to unﬂinchingly fulﬁll its responsibility to provide high-level, advanced medical care, working toward resolution of community problems through education,
research and medical activities, as well as to develop the diagnostic radiology professionals who will bear the
responsibility for providing community medical care, and to develop professionals who have a global outlook
and can ﬂourish in this age of globalization. More than ever, advancing the fundamental medical concepts ofpatient-oriented medical care and thorough medical safety management are core principles in the ﬁeldof diagnostic radiology and nuclear medicine, so continuing to maintain capabilities from this perspective isessential in daily practice.
Under the new radiologist system, it is possible to obtain a qualiﬁcation by completing two years of postgraduate clinical training, followed by three years of general training at a training facility approved by theJapan Radiological Society , then sitting the radiologist examination (sixth year after graduation). After passing that examination, it is then possible to obtain a qualiﬁcation in either radiotherapy or diagnostic radiology
by completing a further two years of specialist training and sitting either the radiotherapist or the diagnostic
radiologist examination (eighth year after graduation).
Diagnostic radiology and nuclear medicine was divided oﬀ the specialist ﬁeld responsible for diagnostic ra-― 418 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

diology in July 2013. However, because the radiologist examination covers both treatment and diagnosis, theplan for the three years of general training is to provide it without dividing students into treatment or diagnosis
streams. In compliance with the specialist training curriculum guidelines set out by the Japan RadiologicalSociety, students generally complete about one year of training in the university, then about two years of training in an external aﬃliated hospital. There are currently 15 external aﬃliated hospitals approved by JRS as
training hospitals. When commencing specialist training, students are allocated to their specialist ﬁelds. Afterthe two years of specialist training, all students decide whether to aim to become a radiotherapist or a diagnostic
radiologist. Almost all then set out to obtain a further degree by enrolling in either a post-graduate school oradult graduate school. In addition, many also obtain certiﬁcation as a senior (ﬁrst class) radiation protection
supervisor.
In recent times, diagnostic radiology has been experiencing an increasing load in terms of image processing, the number of image readings, and server storage, owing to improvements in instrument performance. As
hybrid imaging such as PET/CT, SPECT/CT, PET/MRI become more prevalent, the diversiﬁcation of diagnostic methods is accelerating. This trend is expected to continue, so there is a need for work on adequate
personnel responses, including infrastructure improvement. And because the combined use of functional images
to monitor metabolism and blood ﬂow from morphologic images alone will be fundamental, it is essential thatthe university goes on enhancing education for radiologists so that they acquire the capabilities to extract andanalyze clinically useful information from the complex data gathered.
Remote diagnostic imaging is a good example of this. In regard to its responsibility to contribute to thecommunity, there have been changes in the way diagnostic radiology today has been active in society. The
community gives special privileges to the diagnosing doctor, including the exclusive or primary responsibility
to provide speciﬁed medical services. The university must unwaveringly fulﬁll its mission as such by providing
advanced medical care through medical practice, as well as developing the doctors who will provide healthcare
to communities. Although it could not be claimed that the environment surrounding diagnostic radiology incommunity healthcare has fully matured, as specialists, it is necessary to contribute to the development of local
communities and to exercise the privileges granted as specialists: we should carry out our responsibilities while
ﬁrmly holding to this approach.

(2)

Research

Diagnostic specialists must keep up with the latest research in their ﬁelds, applying medical research ﬁndings
to clinical practice, and making use of continuing education, continuing professional development programs,
medical journals, society activities and the internet to maintain their skills. Along with the importance of
maintaining awareness of how to interpret and apply research ﬁndings to the patient, it is also necessary to go
on formulating broad education programs from a specialist perspective, in order to stay well informed about thefundamentals of research methods and to practice appropriate medicine. By managing the faculty eﬀectively,
it will go on clarifying radiological perspectives designed to eﬀect inter-disciplinary research activities, taking awhole-university outlook that crosses over the limits of individual departments or graduate schools. The faculty
ensures the education and research activities at graduate schools are reﬂected in the departments while alsoenergetically tackling strategies to secure external funding and strengthen industry collaborations, with theaim of further improving research results. The faculty continues to actively advance international cooperative
networks with the Radiological Society of North America (RSNA), the European Congress of Radiology (ECR),
the Society of Nuclear Medicine and Molecular Imaging (SNMMI), etc. from the standpoint of diagnostic
radiology and nuclear medicine, and continues to advance research based on a thorough awareness of the
impacts emerging in the ﬁeld and the potentialities developing in related practical application ﬁelds.
Diagnostic radiology provides diagnoses by extracting information about the morphology of organs andtissues, three-dimensional structures. It is important in terms of learning to systematically organize that
information for comparison of imaging study analyses with the reference pathological tissue. Within that, using
CT or MRI for tissue characterization that reﬂects the macro-pathology is important for identifying diseases.
Diagnostic radiology is a discipline in which it is possible to zero in on understanding of a pathologicalcondition by collecting and analyzing blood ﬂow and metabolic data over time. The faculty is continuingresearch into a minimally invasive method of extracting in vivo blood ﬂow data to enable the use of in vivo
dynamic analysis as a biomarker with formulation of dynamic scan protocols that obtain images over time withbolus contrast injection before high-resolution, multi-slice CT or high magnetic ﬁeld MRI. Texture analysis isapplied to both of anatomic and functional imaging modalities. We investigate from ﬁrst order (kurtosis) tohigh order (NGLCM、NGTDM、GLSZM). The faculty is also formulating scan protocols that obtain images overtime with 3D PET/CT, as a minimally invasive technique of extracting in vivo metabolic data. Known tracers― 419 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

include 18F-FDG (glucose metabolism), 11C-choline (lipid metabolism), 11C-acetate (lipid metabolism), 18FFAZA (hypoxia), 18F-FACBC (amino acid metabolism), 62/64Cu-ATSM (redox), 18F-FLT (DNA synthesis),
18F-NaF (bone metabolism), 68Ga-DOTATATE (somatostatin receptor), and 68Ga-DOTA-PEG-RGD/68GaDOTA-RGD (angiogenesis). The usefulness of multiple tracers in the discipline of oncology has been observedin numerous carcinomas. As for 68Ga-DOTATATE, theranostics is applied to introduce 177Lu-DOTATATEPRRT. Given the need for examination with standardized imaging and assessment techniques, by conducting
a multi-center joint study using PET/CT, the faculty is working to realize and to formulate methodologies forstandardization to facilitate participation in global clinical trials in Japan.

(3)

Education

In order to meet the expectations of both the patient and diagnostic radiologist, it is important to know thevalues, especially empathy, capabilities, and autonomy that lie at the core of medical care, and to continue toindependently demonstrate them. Diagnostic radiology demands high-level capabilities, so extensive training
is required to acquire those capabilities, while keeping a watch on developments in medical knowledge andmaintaining those capabilities is also an issue for the discipline. Similarly, it is also necessary to maintainknowledge, skills and capabilities in ethics, not only radiology knowledge and skills, in order to respond tochanges in medical practices as well as the social and political environment. Education in the department
aims to develop professionals equipped with the capabilities to resolve a range of problems and the attitude to
identify and research topics themselves, by developing three subject groups, problem presentation, technical skill
acquisition, and collaboration with the profession, and by formulating and implementing specialist education
based on those groups, from the radiological perspective. Education in the postgraduate school aims to develop
professionals who can resolve the problems faced by humanity from a global viewpoint, implementing research
into leading-edge topics within a framework for research guidance under numerous teachers in addition to
the acquisition of specialist knowledge, delivering education that develops inventive and practical researchcapabilities, from the radiological perspective. An essential aspect of postgraduate school education is theongoing maintenance of capabilities from that perspective.
Under the new radiology specialist system, it is possible to obtain a qualiﬁcation by completing two yearsof post-graduate clinical training, followed by three years of general training at a training facility approved by
the Japan Radiological Society , then sitting the radiology specialist examination (sixth year after graduation).
After passing that examination, it is possible to obtain a qualiﬁcation in either radiotherapy or diagnostic
radiology by completing a further two years of specialist training and sitting either the radiotherapist or the
diagnostic radiologist examination (eighth year after graduation).
In July 2013, radiation oncology was divided into diagnostic radiology and nuclear medicine responsible fordiagnostic radiology and radiotherapeutic oncology, in turn responsible for radiotherapy. However, because theradiologist examination covers both treatment and diagnosis, the plan for the three years of general training isto provide the training without dividing students into treatment or diagnosis streams. In compliance with thespecialist training curriculum guidelines set out by the Japan Radiological Society, students generally complete
about one year of training in the university, then about two years of training in an external aﬃliated hospital.
There are currently 11 external aﬃliated hospitals (ﬁve in Tokyo and six in the Kanto region) approved by
the Society as training hospitals. When students commence specialist training, they will be allocated to their
specialist ﬁelds. After the two years of specialist training, all students decide whether to aim to become aradiotherapist or a diagnostic radiologist. Almost all students then set out to obtain a further degree byenrolling in either a post-graduate school or adult graduate school. From the standpoint of managing sealed
and unsealed sources in nuclear medicine, many students also obtain certiﬁcation as a senior (ﬁrst class) radiation
protection supervisor before engaging in clinical and research work.

(4)

Lectures & Courses

The department delivers education based on the university s fundamental policy aimed at realization ofits mission: to contribute to the development of society, with a speciﬁc mission to bear the responsibility forthe basic functions of education, research and medical care. As a department at the core of medical care, it
develops professionals who can engage in practice across diﬀerent ﬁelds, taking the approach that the standardsof behavior demonstrated by diagnostic radiologists in clinical practice have far more impact than the formal
curriculum in ethics.

― 420 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

The department develops professionals who can continue to work to resolve the issues faced by the university
hospital, professionals who know the values, especially empathy, capabilities, and autonomy that lie at thecore of medical care to meet the expectations of both the patient and student, can independently demonstratethem, and can ﬂourish while maintaining a global outlook. Education in the department aims to develop
professionals equipped with the capabilities to resolve a range of problems and the attitude to identify andresearch topics themselves, by developing three subject groups, problem presentation, technical skill acquisition,and collaboration with the profession, and by formulating and implementing specialist education based on thosegroups, from the radiological perspective. Education in the postgraduate school aims to develop professionals
who can resolve the problems faced by humanity from a global perspective, implementing research into leadingedge topics within a framework for research guidance under numerous teachers in addition to the acquisition of
specialist knowledge, delivering education that develops inventive and practical research capabilities, from theradiological perspective.

(5)

Clinical Services & Other Works

Diagnostic Radiology· CT: A total of three CT scanners are involved in diagnostic radiology: two in the radiology department (64-slice
MDCT) and one in the ER center (16-slice MDCT). Not only has the number of examinations using MDCT
increased, but it has been possible to obtain improved diagnostic performance by reading MPR (multi-planarreconstruction) images and 1-mm thick images.
· MRI: A total of four MRI scanners are involved in diagnostic radiology: two 1.5-tesla scanners and two 3-teslascanners. This has allowed for an increase in examinations.
· Ultrasound: The main examinations carried out by diagnostic radiologists are breast and abdominal examinations.
· Angiography and Interventional Radiology (IVR): In the vascular area: TAE for hepatic carcinoma, PTA andstent placement for occlusive arterial disease, intraarterial injection for pelvic tumor, and emergency hemostasis
for ER center patients. In the non-vascular area: mainly CT-guided chest biopsy, breast mass biopsy and lymph
node biopsy.
· Breast: The department is responsible for breast diagnostic radiology and collaborates with the breast surgery
department in team medical care. The department endeavors to diagnose breast cancer at an early stage, provide
accurate pre-surgery diagnoses, and formulate treatment plans by providing high-quality diagnostic radiology
services combining mammography reading, ultrasound examination, as well as MRI and FDG-PET/CT, in
addition to providing image-guided biopsy and surgical marking.
· Conferences: The department holds inter-disciplinary conferences with all departments on a daily basis. The
department holds its internal conference every Friday and participates in externally conducted conferences,
as appropriate. The department also takes a lead role in organizing conferences aimed at external attendees(Shoheizaka Radiology: twice a year), and the three-university joint conferences (three times a year).
Nuclear Medicine· Since the department began operating its second PET/CT scanner in November 2006, it has been conducting
15 to 16 PET examinations per day, mainly for malignant tumor, as well as eight to 10 general radioisotope
examinations per day, mainly brain and myocardial SPECT. PET/CT for radiation planning and C-11 acetate
PET/CT for hematologic malignancies were introduced in 2016.

(6)

Clinical Performances

Being a core diagnosis and treatment department, diagnostic radiology and nuclear medicine is a departmentthat engages in inter-disciplinary clinical practice forming strong partnerships to meet the needs of its internal client departments and works to resolve issues faced by the university hospital, bearing its responsibility
to unswervingly fulﬁll its mission from a global perspective. Diagnostic radiology and nuclear medicine isequipped with the capabilities to process large volumes of imaging information, it develops problem presentation, technical skill acquisition, and collaboration with all departments, and possesses the characteristics to go
on conscientiously tackling new modalities, probes and contrasts as well as clinical trials.

― 421 ―

Advanced Therapeutic Sciences(7)

Advanced Therapeutic Sciences

Publications[Original Articles]
1. Shibata H, Kato S, Sekine I, Abe K, Araki N, Iguchi H, Izumi T, Inaba Y, Osaka I, Kato S, Kawai
A, Kinuya S, Kodaira M, Kobayashi E, Kobayashi T, Sato J, Shinohara N, Takahashi S, Takamatsu Y,
Takayama K, Takayama K, Tateishi U, Nagakura H, Hosaka M, Morioka H, Moriya T, Yuasa T, Yurikusa
T, Yomiya K, Yoshida M. Diagnosis and treatment of bone metastasis: comprehensive guideline of theJapanese Society of Medical Oncology, Japanese Orthopedic Association, Japanese Urological Association,
and Japanese Society for Radiation Oncology. ESMO Open. 2016; 1(2); e000037
2. Yoshio Kitazume, Shin-ichi Taura, Shuichiro Nakaminato, Osamu Noguchi, Yukiyoshi Masaki, Ichiro
Kasahara, Mitsuhiro Kishino, Ukihide Tateishi. Diﬀusion-Weighted Magnetic Resonance Imaging to Differentiate Malignant from Benign Gallbladder Disorders European Journal of Radiology. 2016;
3. Matsuhisa Akiyuki, Kishino Mitsuhiro, Nakadate Masashi, Kudo Toshifumi, Inoue Yoshinori, Tateishi
Ukihide. Embolosclerotherapy by the Transvenous Approach for Lower Extremity Arteriovenous Malformation in Cowden Syndrome: A Case Report. Ann Vasc Dis. 2016; 9(1); 66-69
4. Uehara Takeaki, Takeno Mitsuhiro, Hama Maasa, Yoshimi Ryusuke, Suda Akiko, Ihata Atsushi, Ueda
Atsuhisa, Tateishi Ukihide, Ishigatsubo Yoshiaki. Deep-inspiration breath-hold (18)F-FDG-PET/CT isuseful for assessment of connective tissue disease associated interstitial pneumonia. Mod Rheumatol.
2016.01; 26(1); 121-127
5. Yamada I, Hikishima K, Miyasaka N, Kato K, Kojima K, Kawano T, Ito E, Kobayashi D, Eishi Y, Okano
H. Gastric carcinoma: evaluation with diﬀusion-tensor MR imaging and tractography ex vivo. Magn
Reson Imaging. 2016.02; 34(2); 144-151
6. Yoshiaki Katada, Shunichi Kondo, Eitoshi Tsuboi, Kyu Rokkaku, Yoshihito Irie, Hitoshi Yokoyama. Endovascular Total Arch Repair Using In Situ Fenestration for Arch Aneurysm and Chronic Type A Dissection. Ann. Thorac. Surg.. 2016.02; 101(2); 625-630
7. Nakaigawa Noboru, Kondo Keiichi, Tateishi Ukihide, Minamimoto Ryogo, Kaneta Tomohiro, Namura
Kazuhiro, Ueno Daiki, Kobayashi Kazuki, Kishida Takeshi, Ikeda Ichiro, Hasumi Hisashi, Makiyama
Kazuhide, Kubota Yoshinobu, Inoue Tomio, Yao Masahiro. FDG PET/CT as a prognostic biomarker inthe era of molecular-targeting therapies: max SUVmax predicts survival of patients with advanced renal
cell carcinoma. BMC Cancer. 2016.02; 16; 67
8. Torii Ikuo, Tateishi Ukihide, Terauchi Takashi, Inoue Tomio. Prognostic implications of diﬀusion-weighted
magnetic resonance imaging in patients with superior sulcus tumors receiving induction chemoradiation
therapy. Jpn J Clin Oncol. 2016.03; 46(3); 264-269
9. Oyama Jun, Mori Kouichi, Imamura Masatoshi, Mizushima Yukiko, Tateishi Ukihide. Size of the intracranial optic nerve and optic tract in neonates at term-equivalent age at magnetic resonance imaging.
Pediatr Radiol. 2016.04; 46(4); 527-533
10. Kazuo Ohtsuka, Kento Takenaka, Yoshio Kitazume, Toshimitsu Fujii, Katsuyoshi Matsuoka, Maiko
Kimura, Takashi Nagaishi, Mamoru Watanabe. Magnetic resonance enterography for the evaluationof the deep small intestine in Crohn’s disease. Intest Res. 2016.04; 14(2); 120-126
11. Haruka Maneyama, Naoyuki Miyasaka, Kimio Wakana, Megumi Nakamura, Yoshio Kitazume, Toshiro
Kubota. Vanishing intravenous leiomyomatosis after hysterectomy: Assessment of the need to perform
complete resection. J. Obstet. Gynaecol. Res.. 2016.04;
12. Iwasa Y, Kitazume Y, Tateishi U, Saida Y, Ban D, Tanabe M, Takemoto A.. Hepatocellular Carcinoma
Histological Grade Prediction: A Quantitative Comparison of Diﬀusion-Weighted, T2-Weighted, andHepatobiliary-Phase Magnetic Resonance Imaging. J Comput Assist Tomogr. . 2016.05; 40(3); 463470
13. Mitsuhiro Tozaki, Yoshifumi Kuroki, Mari Kikuchi, Yasuyuki Kojima, Kazunori Kubota, Hiroshi Nakahara, Yoshinori Ito, Hirofumi Mukai. The Japanese Breast Cancer Society clinical practice guidelines forscreening and imaging diagnosis of breast cancer, 2015 edition Breast Cancer. 2016.06;― 422 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

14. Sahoko Chiba, Kimitake Tsuchiya, Takumi Akashi, Masahiro Ishizuka, Tsukasa Okamoto, Haruhiko Furusawa, Tomoya Tateishi, Mitsuhiro Kishino, Yasunari Miyazaki, Ukihide Tateishi, Tamiko Takemura,
Naohiko Inase. Chronic Hypersensitivity Pneumonitis With a Usual Interstitial Pneumonia-Like Pattern:
Correlation Between Histopathologic and Clinical Findings. Chest. 2016.06; 149(6); 1473-1481
15. Hibi Toshifumi, Hirohata Shunsei, Kikuchi Hirotoshi, Tateishi Ukihide, Sato Noriko, Ozaki Kunihiko,
Kondo Kazuoki, Ishigatsubo Yoshiaki. Inﬂiximab therapy for intestinal, neurological, and vascular involvement in Behcet disease: Eﬃcacy, safety, and pharmacokinetics in a multicenter, prospective, open-label,
single-arm phase 3 study. Medicine (Baltimore). 2016.06; 95(24); e3863
16. Nakadate Masashi, Miyamoto Norikazu, Starkey Jay, Toriihara Akira, Tateishi Ukihide. Anterograde
Degeneration of the Nigrostriatal Pathway Visualized by 123I-FP-CIT SPECT in a Patient With Arteryof Percheron Infarction. Clin Nucl Med. 2016.06; 41(6); 483-484
17. Yamada I, Hikishima K, Miyasaka N, Kato K, Ito E, Kojima K, Kawano T, Kobayashi D, Eishi Y, Okano
H. q-Space MR imaging of gastric carcinoma ex vivo: correlation with histopathologic ﬁndings. Magn
Reson Med. 2016.08; 76(2); 602-612
18. Tomoyuki Fujioka, Kazunori Kubota, Akira Toriihara, Youichi Machida, Kaori Okazawa, Tsuyoshi Nakagawa, Yukihisa Saida, Ukihide Tateishi. Tumor characteristics of ductal carcinoma in situ of breast
visualized on [ F-18] ﬂuorodeoxyglucose-positron emission tomography/computed tomography: Resultsfrom a retrospective study. World J Radiol. 2016.08; 8(8); 743-749
19. Nozaki T, Tasaki A, Horiuchi S, Ochi J, Starkey J, Hara T, Saida Y, Yoshioka H. Predicting Retear after
Repair of Full-Thickness Rotator Cuﬀ Tear: Two-Point Dixon MR Imaging Quantiﬁcation of Fatty Muscle
Degeneration-Initial Experience with 1-year Follow-up. Radiology.. 2016.08; 280(2); 500-509
20. Nozaki T, Morita Y, Hasegawa D, Makidono A, Yoshimoto Y, Starkey J, Kusakawa I, Manabe A, Saida
Y.. Cervical ultrasound and computed tomography of Kawasaki disease: Comparison with lymphadenitis.
Pediatr Int.. 2016.11; 58(11); 1146-1152
21. Kamo Minobu, Nozaki Taiki, Yoshida Kyoko, Tateishi Ukihide, Akita Keiichi. Kinking of the upper ureterin CT urography: anatomic and clinical signiﬁcance. Surg Radiol Anat. 2016.12; 38(10); 1115-1121
22. Masashi Nakadate, Norikazu Miyamoto, Jay Starkey, Akira Toriihara, Ukihide Tateishi. Anterograde
degeneration of the nigrostriatal pathway visualized by 123I-FP-CIT SPECT in patient with artery ofPercheron infarction. Clin Nucl Med. 41; 483-484
23. Yojiro Umezaki, Anna Miura, Motoko Watanabe, Miho Takenoshita, Akihito Uezato, Akira Toriihara,
Toru Nishikawa, Akira Toyofuku. Oral cenesthopathy Biopsychosoc Med. 10; 20[Books etc]
1. Fukui Tsugiya, Nara Nobuo,Yukihisa Saida et al. . Medical Diagnosis 3rd ed.. 2016.02 (ISBN : 978-4260-02064-0)[Conference Activities & Talks]
1. T. Fujioka, E. Yamaga, K. Kubota, M. Kishino, Y. Kitazume, Y. Saida, U. Tateishi. Gastrointestinal
tumor syndrome; What radiologist should know!!. European Congress of Radiology 2016.03
2. Kazunori Kubota, Tomoyuki Fujioka, Kaori Okazawa, Makiko Honda, Emi N Yamaga, Akira Toriihara,
Yukihisa Saida, Ukihide Tateishi. Intratumoral heterogeneity of Breast Cancer in Diagnostic Imaging.
ECR 2016 2016.03.02
3. Kazunori Kubota, Akira Toriihara, Kaori Okazawa, Masashi Nakadate, Tomoyuki Fujioka, Yoshihiro
Iwasa, Emi N Yamaga, Yukihisa Saida, Ukihide Tateishi. FDG-PET/CT Features of Intestinal Intussusception. ECR 2016 2016.03.02
4. Yoshio Kitazume , Tomoyuki Fujioka , Kento Takenaka, Toshimitsu Fujii, Kazuo Ohtsuka, Yukihisa Saida,
Ukihide Tateishi. Can magnetic resonance enterocolonography reﬂect the presence of partial mucosal
healing in patients with Crohn’s disease?. European Congress of Radiology 2016.03.02 Vienna― 423 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

5. Tomoyuki Fujioka, Emi Yamaga, Kazunori Kubota Akira Toriihara, Yukihisa Saida, Ukihide Tateishi.
Diagnostic imaging of olfactory disorder: review according to the ’pathway of smell’. The 4th Congress of
the Japan Oncoplastic Breast Surgery Society 2016.04 Yokohama
6. Akira Toriihara, Masashi Nakadate, Tomoyuki Fujioka, Jun Oyama, Atsunobu Tsunoda, Takuro Sumi,
Ukihide Tateishi. Utility of pretherapeutic FDG-PET/CT for evaluating cancer of external auditory
canal.. JRS 75th Annual Meeting 2016.04.14 Yokohama
7. Yoshio Kitazume , Tomoyuki Fujioka , Kento Takenaka, Kazuo Ohtsuka, Yukihisa Saida, Ukihide Tateishi.
Magnetic Resonance Enterocolonography Reﬂects the Presence of Partial Mucosal Healing in Patients with
Crohn’s Disease. The 75th Annual Meeting of the Japan Radiological Society 2016.04.16
8. Kazunori Kubota, Tomoyuki Fujioka, Kaori Okazawa, Akira Toriihara, Makiko Honda, Emi N Yamaga,
Yukihisa Saida, Ukihide Tateishi. Imaging phenotypes of 18F-FDG PET/CT and MRI in Predicting theClinicopathol ogic Subtypes of Triple Negative Breast Cancers.. The 75th Annual Meeting of the Japan
Radiological Society 2016.04.16
9. Saida Yukihisa, Yamagiwa Ken, Ito Takashi. Grooves on the hepatic surface: Corset liver or anatomicalvariants?. GJRA meeting 2016.06
10. Akira Toriihara, Shin Nakamura, Akira Toyofuku, Ukihide Tateishi. Usefulness of molecular imaging forpatients with dental diseases.. SNMMI 63rd Annual Meeting 2016.06.12
11. Yukihisa Saida,Ken Yamagiwa,Namiko Matsui,Tomoyuki Fujioka,Yoshihiro Iwasa,Mitsuhiro Kishino,Takashi
Ito. Consideration of the etiology of the accessory hepatic ﬁssures.. The 32nd Annual Meeting of JapaneseSociety of Abdominal Radiology 2016.06.24
12. Yoshiaki Katada, Yoshihito Irie, Shunichi Kondo, Hitoshi Nakanowatari, Yoshiki Endo, Hitoshi Yokoyama.
Transcatheter plug closure of mitral paravalvular leaks by transapical approach.. ISMICS2016 winterworkshop 2016.10
13. Yamada I, Yoshino N, Hikishima K, Miyasaka N, Yamauchi S, Uetake H, Yasuno M, Saida Y, Tateishi
U, Kobayashi D, Eishi Y. Colorectal Carcinoma: Ex Vivo Evaluation with 3-T High-Spatial-Resolution
Quantitative T2 Mapping–Correlation with Histopathologic Findings (Yamada I, et al.). The 102nd
Scientiﬁc Assembly and Annual Meeting of the Radiological Society of North America 2016.11 Chicago,USA― 424 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

Human Genetics and Disease Diversity

Professor, Toshihiro Tanaka
Project Assistant Professor, Ryo Watanabe(1)

Research

1) Elucidation of genetic architecture of human metabolic diseases using genome and meta-genome information
2) Identiﬁcation of biomarkers for personalized medicine
3) Pharmacogenomics
4) Functional genomics
5) Statistical genetics and genome drug discovery(2)

Lectures & Courses

As we say Every human is diﬀerent , human genetic diversity has essential impacts on clinical ﬁelds, e.g.
disease risk, clinical eﬃcacy, and drug responses. Our laboratory aims to elucidate the diversity of humanbeing through comprehensive research activities including genome and epi-genome analyses of human diseases,
methodological development of statistical genetics, and human resources cultivation to achieve personalized
medicine.
(3)

Publications[Original Articles]
1. Imamura Minako, Takahashi Atsushi, Yamauchi Toshimasa, Hara Kazuo, Yasuda Kazuki, Grarup Niels,
Zhao Wei, Wang Xu, Huerta-Chagoya Alicia, Hu Cheng, Moon Sanghoon, Long Jirong, Kwak Soo Heon,
Rasheed Asif, Saxena Richa, Ma Ronald C W, Okada Yukinori, Iwata Minoru, Hosoe Jun, Shojima
Nobuhiro, Iwasaki Minaka, Fujita Hayato, Suzuki Ken, Danesh John, Jorgensen Torben, Jorgensen Marit
E, Witte Daniel R, Brandslund Ivan, Christensen Cramer, Hansen Torben, Mercader Josep M, Flannick
Jason, Moreno-Macias Hortensia, Burtt Noel P, Zhang Rong, Kim Young Jin, Zheng Wei, Singh Jai Rup,
Tam Claudia H T, Hirose Hiroshi, Maegawa Hiroshi, Ito Chikako, Kaku Kohei, Watada Hirotaka, Tanaka
Yasushi, Tobe Kazuyuki, Kawamori Ryuzo, Kubo Michiaki, Cho Yoon Shin, Chan Juliana C N, Sanghera
Dharambir, Frossard Philippe, Park Kyong Soo, Shu Xiao-Ou, Kim Bong-Jo, Florez Jose C, Tusie-Luna
Teresa, Jia Weiping, Tai E Shyong, Pedersen Oluf, Saleheen Danish, Maeda Shiro, Kadowaki Takashi.
Genome-wide association studies in the Japanese population identify seven novel loci for type 2 diabetes.
Nat Commun. 2016; 7; 10531
2. Okada Yukinori, Muramatsu Tomoki, Suita Naomasa, Kanai Masahiro, Kawakami Eiryo, Iotchkova
Valentina, Soranzo Nicole, Inazawa Johji, Tanaka Toshihiro. Signiﬁcant impact of miRNA-target gene
networks on genetics of human complex traits. Sci Rep. 2016; 6; 22223
3. Onouchi Yoshihiro, Fukazawa Ryuji, Yamamura Kenichiro, Suzuki Hiroyuki, Kakimoto Nobuyuki, Suenaga Tomohiro, Takeuchi Takashi, Hamada Hiromichi, Honda Takafumi, Yasukawa Kumi, Terai Masaru,
Ebata Ryota, Higashi Kouji, Saji Tsutomu, Kemmotsu Yasushi, Takatsuki Shinichi, Ouchi Kazunobu,
Kishi Fumio, Yoshikawa Tetsushi, Nagai Toshiro, Hamamoto Kunihiro, Sato Yoshitake, Honda Akihito,― 425 ―

Advanced Therapeutic
TherapeuticSciences
Sciences
Advanced

Kobayashi Hironobu, Sato Junichi, Shibuta Shoichi, Miyawaki Masakazu, Oishi Ko, Yamaga Hironobu,
Aoyagi Noriyuki, Yoshiyama Megumi, Miyashita Ritsuko, Murata Yuji, Fujino Akihiro, Ozaki Kouichi,
Kawasaki Tomisaku, Abe Jun, Seki Mitsuru, Kobayashi Tohru, Arakawa Hirokazu, Ogawa Shunichi, Hara
Toshiro, Hata Akira, Tanaka Toshihiro. Variations in ORAI1 Gene Associated with Kawasaki Disease.
PLoS One. 2016; 11(1); e0145486
4. Atsuko Konta, Kouichi Ozaki, Yasuhiko Sakata, Atsushi Takahashi, Takashi Morizono, Shinichiro Suna,
Yoshihiro Onouchi, Tatsuhiko Tsunoda, Michiaki Kubo, Issei Komuro, Yoshinobu Eishi, Toshihiro Tanaka.
A functional SNP in FLT1 increases risk of coronary artery disease in a Japanese population. J. Hum.
Genet.. 2016.01;
5. Hirofumi Zempo, Jun-Ichi Suzuki, Masahito Ogawa, Ryo Watanabe, Mitsuaki Isobe. A diﬀerent role ofangiotensin II type 1a receptor in the development and hypertrophy of plantaris muscle in mice. J. Appl.
Genet.. 2016.02; 57(1); 91-97
6. Sun Celi, Molineros Julio E, Looger Loren L, Zhou Xu-Jie, Kim Kwangwoo, Okada Yukinori, Ma Jianyang,
Qi Yuan-Yuan, Kim-Howard Xana, Motghare Prasenjeet, Bhattarai Krishna, Adler Adam, Bang SoYoung, Lee Hye-Soon, Kim Tae-Hwan, Kang Young Mo, Suh Chang-Hee, Chung Won Tae, Park YongBeom, Choe Jung-Yoon, Shim Seung Cheol, Kochi Yuta, Suzuki Akari, Kubo Michiaki, Sumida Takayuki,
Yamamoto Kazuhiko, Lee Shin-Seok, Kim Young Jin, Han Bok-Ghee, Dozmorov Mikhail, Kaufman Kenneth M, Wren Jonathan D, Harley John B, Shen Nan, Chua Kek Heng, Zhang Hong, Bae Sang-Cheol, Nath
Swapan K. High-density genotyping of immune-related loci identiﬁes new SLE risk variants in individuals
with Asian ancestry. Nat Genet. 2016.03; 48(3); 323-330
7. Shiheido Y, Maejima Y, Suzuki J, Aoyama N, Kaneko M, Watanabe R, Sakamaki Y, Wakayama K,
Ikeda Y, Akazawa H, Ichinose S, Komuro I, Izumi Y, Isobe M. Porphyromonas gingivalis, a periodontal
pathogen, enhances myocardial vulnerability, thereby promoting post-infarct cardiac rupture. J. Mol.
Cell. Cardiol.. 2016.04;
8. Kouichi Ozaki, Toshihiro Tanaka. [ Molecular genetics of coronary artery diseases] . Nippon Rinsho.
2016.06; 74 Suppl 4 Pt 1; 306-313
9. Taniguchi, JB., Kondo, K., Fujita, K., Chen, X., Homma, H., Sudo, T., Mao, Y., Watase, K., Tanaka, T.,
Tagawa, K., Tamura, T., Muramatsu, SI., Okazawa, H.. RpA1 ameliorates symptoms of mutant Ataxin-1
knock-in mice and enhances DNA damage repair. Hum Mol Genet.. 2016.08;
10. Kaneko M, Suzuki J, Aoyama N, Watanabe R, Yoshida A, Shiheido Y, Izumi Y, Isobe M. Toll-like receptor2 has a critical role in periodontal pathogen-induced myocardial ﬁbrosis in the pressure-overloaded murine
hearts. Hypertens. Res.. 2016.09; e-pub;
11. S-W Chang, C W McDonough, Y Gong, T A Johnson, T Tsunoda, E R Gamazon, M A Perera, A
Takahashi, T Tanaka, M Kubo, C J Pepine, J A Johnson, R M Cooper-DeHoﬀ. Genome-wide association
study identiﬁes pharmacogenomic loci linked with speciﬁc antihypertensive drug treatment and new-onset
diabetes. Pharmacogenomics J.. 2016.09;
12. Nobue Yagihara, Hiroshi Watanabe, Phil Barnett, Laetitia Duboscq-Bidot, Atack C Thomas, Ping Yang,
Seiko Ohno, Kanae Hasegawa, Ryozo Kuwano, Stéphanie Chatel, Richard Redon, Jean-Jacques Schott,Vincent Probst, Tamara T Koopmann, Connie R Bezzina, Arthur A M Wilde, Yukiko Nakano, Takeshi
Aiba, Yoshihiro Miyamoto, Shiro Kamakura, Dawood Darbar, Brian S Donahue, Daichi Shigemizu, Toshihiro Tanaka, Tatsuhiko Tsunoda, Masayoshi Suda, Akinori Sato, Tohru Minamino, Naoto Endo, Wataru
Shimizu, Minoru Horie, Dan M Roden, Naomasa Makita. Variants in the SCN5A Promoter AssociatedWith Various Arrhythmia Phenotypes. J Am Heart Assoc. 2016.09; 5(9);13. Masahiro Kanai, Toshihiro Tanaka, Yukinori Okada. Empirical estimation of genome-wide signiﬁcancethresholds based on the 1000 Genomes Project data set. J. Hum. Genet.. 2016.10; 61(10); 861-866
14. Tumurkhuu T, Fujiwara T, Komazaki Y, Kawaguchi Y, Tanaka T, Inazawa J, Ganburged G, Bazar A,
Ogawa T, Moriyama K. Association between maternal education and malocclusion in Mongolian adolescents: a cross-sectional study. BMJ Open. 2016.11; 6(11);15. Lian Liu, Yusuke Ebana, Jun-Ichi Nitta, Yoshihide Takahashi, Shinsuke Miyazaki, Toshihiro Tanaka,
Masatoshi Komura, Mitsuaki Isobe, Tetsushi Furukawa. Genetic Variants Associated With Susceptibility
to Atrial Fibrillation in a Japanese Population. Can J Cardiol. 2016.11;― 426 ―

Advanced
AdvancedTherapeutic
Therapeutic Sciences
Sciences

16. Hirofumi Zempo, Jun-Ichi Suzuki, Masahito Ogawa, Ryo Watanabe, Katsuhito Fujiu, Ichiro Manabe,
Simon J Conway, Yoshiaki Taniyama, Ryuichi Morishita, Yasunobu Hirata, Mitsuaki Isobe, Ryozo Nagai.
Inﬂuence of periostin-positive cell-speciﬁc Klf5 deletion on aortic thickening in DOCA-salt hypertensive
mice. Hypertens. Res.. 2016.11; 39(11); 764-768
17. Tsasan Tumurkhuu, Takeo Fujiwara, Yuko Komazaki, Yoko Kawaguchi, Toshihiro Tanaka, Johji Inazawa,
Ganjargal Ganburged, Amarsaikhan Bazar, Takuya Ogawa, Keiji Moriyama. Association between maternal education and malocclusion in Mongolian adolescents: a cross-sectional study. BMJ Open. 2016.11;
6(11); e012283
18. Mari Ichikawa, Takeshi Aiba, Seiko Ohno, Daichi Shigemizu, Junichi Ozawa, Keiko Sonoda, Megumi
Fukuyama, Hideki Itoh, Yoshihiro Miyamoto, Tatsuhiko Tsunoda, Takeru Makiyama, Toshihiro Tanaka,
Wataru Shimizu, Minoru Horie. Phenotypic Variability of ANK2 Mutations in Patients With Inherited
Primary Arrhythmia Syndromes. Circ. J.. 2016.11; 80(12); 2435-2442
19. Chisato Shimizu, Hariklia Eleftherohorinou, Victoria J Wright, Jihoon Kim, Martin P Alphonse, JamesC Perry, Rolando Cimaz, David Burgner, Nagib Dahdah, Long T Hoang, Chiea Chuen Khor, Andrea Salgado, Adriana H Tremoulet, Sonia Davila, Taco W Kuijpers, Martin L Hibberd, Todd A Johnson, Atsushi
Takahashi, Tatsuhiko Tsunoda, Michiaki Kubo, Toshihiro Tanaka, Yoshihiro Onouchi, Rae S M Yeung,
Lachlan J M Coin, Michael Levin, Jane C Burns, . Genetic Variation in the SLC8A1 Calcium Signaling
Pathway Is Associated With Susceptibility to Kawasaki Disease and Coronary Artery Abnormalities. Circ
Cardiovasc Genet. 2016.12; 9(6); 559-568[Misc]
1. Toshihiro Tanaka, Akinori Kimura. Cardiovascular genetics. J. Hum. Genet.. 2016.01; 61(1); 1[Conference Activities & Talks]
1. Onouchi Y, Nakagawa H, Shigemizu D, Ozaki K, Nakamura Y, Asami Y, Seki M, Kobayashi T, Kochi
Y, Toda T, Satake W, Hata A, Tsunoda T, Tanaka T, Japan Kawasaki Disease Genome Consortium.
Search for genetic variations responsible for giant coronary aneurysms in Kawasaki disease patients bywhole exome sequencing. ICHG2016 2016.04.04
2. Takeaki Sudo, Yukinori Okada, Hiroaki Kobayashi, Misa Gokyu, Yuichi Izumi, Toshihiro Tanaka. Wholeexome sequencing reveals a novel gene as a cause of Aggressive periodontitis in Japanese consanguineousfamilies. The 13th International Congress of Human Genetics 2016.04.04
3. Fares Taie L, Gerber S, Tawara A, Ramirez-Miranda A, Douet JY, Verdin H, Zanteno JC, Kondo H,
Passet B, Yamamoto K, Iwai M, Tanaka T, Nakamura Y, Kimura W, Munnich A, Baere ED, RaymondLetron I, Kaplan J, Calvas P, Roche O, Rozet JM. Submicroscopic deletions at 13q32.1 cause congenital
microcoria. ICHG2016 2016.04.05
4. Ozaki K, Morizono T, Tsunoda T, Kubo M, Tanaka T. Up-regulation of FLT1 by a novel functional SNP
increases risk of coronary artery disease through an inﬂammatory activation. ICHG2016 2016.04.07
5. Kanai M, Tanaka T, Okada Y. Empirical estimation of genome-wide signiﬁcance thresholds based on the1000 Genomes Project dataset. ICHG2016 2016.04.07
6. Kanai M, Okada Y, Muramatsu T, Suita N, Kawakami E, Iotchkova V, Soranzo N, Inazawa J, Tanaka
T. Signiﬁcant impact of miRNA–target gene networks on genetics of human complex traits. ICHG2016
2016.04.07
7. Tanaka T. Exome Analyses of Long QT Syndrome. Symposium20 Annual Meeting of the Japanese Heart
Rhythm Society 2016 2016.07.16 Sapporo Convention Center[Others]
1. Tanaka T. Chairs, 2016.04
Complex traits and polygenic disorders2.
Concurrent Oral Session 20.
ICHG2016― 427 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

Applied Regenerative Medicine

Professor:Ichiro SEKIYAAssistant Professor:Masafumi HORIE, Koji OTABE,
Hisako KATANO
Project Assistant Professor:Nobutake OZEKI,Yusuke NAKAGAWA,
Mitsuru MIZUNO,Keiichiro KOMORI
Graduate Student:Kenta KATAGIRI,Yuji KONO,Naoto WATANABE,
Yoshihisa KUSHIDA,Taihei TODA,Mana NARITOMITechnical Staﬀ：Yukie MATSUMOTO,Shizuka FUJII,
Teruko NAKAMURA
Assistant Clerk:Mika WATANABE,Kimiko TAKANASHI(1)

OutlineOur purpose is to support and advance stem cell research and regenerative medicine for the discovery anddevelopment of cures, therapies, diagnostics and research technologies to relieve human suﬀering from chronic
disease and injury.
(2)

Research

1) Development of regenerative medicine with stem cells.
2) Realization and industrialization of cell and regenerative therapy.
3) Establishment of safety test for regenerative medicine.
4) Translational research.
(3)

Clinical Services & Other Works

We have started a clinical study in that autologous synovial mesenchymal stem cells were transplanted withmeniscus repair to regenerate lateral osteoarthritis of the knee in which the lateral meniscus was extruded andthe cartilage was defected.
(4)

Publications[Original Articles]
1. Yusuke Nakagawa, Ichiro Sekiya, Shimpei Kondo, Takashi Tabuchi, Shizuko Ichinose, Hideyuki Koga,
Kunikazu Tsuji, Takeshi Muneta. Relationship between MRI T1 rho value and histological ﬁndings ofintact and radially incised menisci in microminipigs. J Magn Reson Imaging. 2016.02; 43(2); 434-445
2. Yusuke Nakagawa, Takeshi Muneta, Koji Otabe, Nobutake Ozeki, Mitsuru Mizuno, Mio Udo, Ryusuke
Saito, Katsuaki Yanagisawa, Shizuko Ichinose, Hideyuki Koga, Kunikazu Tsuji, Ichiro Sekiya. Cartilage
Derived from Bone Marrow Mesenchymal Stem Cells Expresses Lubricin In Vitro and In Vivo. PLoS
ONE. 2016.02; 11(2); e0148777― 428 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

3. Chisato Sato, Yoshitaka Iso, Takuya Mizukami, Koji Otabe, Masahiro Sasai, Masaaki Kurata, Takeyuki
Sanbe, Ichiro Sekiya, Akira Miyazaki, Hiroshi Suzuki. Fibroblast growth factor-23 induces cellular senescence in human mesenchymal stem cells from skeletal muscle. Biochem. Biophys. Res. Commun..
2016.02; 470(3); 657-662
4. Shimpei Kondo, Takeshi Muneta, Yusuke Nakagawa, Hideyuki Koga, Toshifumi Watanabe, Kunikazu
Tsuji, Shinichi Sotome, Atsushi Okawa, Shinji Kiuchi, Hideo Ono, Mitsuru Mizuno, Ichiro Sekiya. Transplantation of autologous synovial mesenchymal stem cells promotes meniscus regeneration in aged primates. J. Orthop. Res.. 2016.02;
5. Toshifumi Watanabe, Takeshi Muneta, Kazuyoshi Yagishita, Kenji Hara, Hideyuki Koga, Ichiro Sekiya.
Closed Suction Drainage Is Not Necessary for Total Knee Arthroplasty: A Prospective Study on Simultaneous Bilateral Surgeries of a Mean Follow-Up of 5.5 Years. J Arthroplasty. 2016.03; 31(3); 641-645
6. Katsuaki Yanagisawa, Takeshi Muneta, Nobutake Ozeki, Yusuke Nakagawa, Mio Udo, Ryusuke Saito,
Hideyuki Koga, Kunikazu Tsuji, Ichiro Sekiya. Weekly injections of Hylan G-F 20 delay cartilage degeneration in partial meniscectomized rat knees. BMC Musculoskelet Disord. 2016.04; 17(1); 188
7. Kazumasa Miyatake, Takeshi Muneta, Miyoko Ojima, Jun Yamada, Yu Matsukura, Kahaer Abula, Ichiro
Sekiya, Kunikazu Tsuji. Coordinate and synergistic eﬀects of extensive treadmill exercise and ovariectomy
on articular cartilage degeneration. BMC Musculoskelet Disord. 2016.05; 17(1); 238
8. Mai Katakura, Hideyuki Koga, Kaori Nakamura, Ichiro Sekiya, Takeshi Muneta. Eﬀects of diﬀerent
femoral tunnel positions on tension changes in anterolateral ligament reconstruction. Knee Surg Sports
Traumatol Arthrosc. 2016.05;
9. N Ozeki, T Muneta, H Koga, Y Nakagawa, M Mizuno, K Tsuji, Y Mabuchi, C Akazawa, E Kobayashi, K
Matsumoto, K Futamura, T Saito, I Sekiya. Not single but periodic injections of synovial mesenchymal
stem cells maintain viable cells in knees and inhibit osteoarthritis progression in rats. Osteoarthr. Cartil..
2016.06; 24(6); 1061-1070
10. Hiroki Katagiri, Kaori Nakamura, Toshifumi Watanabe, Hideyuki Koga, Kazuyoshi Yagishita, Ichiro
Sekiya, Takeshi Muneta. Increase of patellofemoral height has decreased maximum knee ﬂexion after
total knee arthroplasty of posterior cruciate-substituting prosthesis in a clinical series. J Orthop Sci.
2016.07; 21(4); 458-462
11. M Udo, T Muneta, K Tsuji, N Ozeki, Y Nakagawa, T Ohara, R Saito, K Yanagisawa, H Koga, I Sekiya.
Monoiodoacetic acid induces arthritis and synovitis in rats in a dose- and time-dependent manner: proposed model-speciﬁc scoring systems. Osteoarthr. Cartil.. 2016.07; 24(7); 1284-1291
12. Takeshi Muneta, Hideyuki Koga, Tomomasa Nakamura, Masafumi Horie, Toshifumi Watanabe, Ichiro
Sekiya. Behind-remnant arthroscopic observation and scoring of femoral attachment of injured anterior
cruciate ligament. Knee Surg Sports Traumatol Arthrosc. 2016.09; 24(9); 2906-2914
13. Koga H, Muneta T, Watanabe T, Mochizuki T, Horie M, Nakamura T, Otabe K, Nakagawa Y, Sekiya I..
Two-Year Outcomes After Arthroscopic Lateral Meniscus Centralization Arthroscopy. 2016.10; 32(10);2000-2008
14. Watanabe T, Muneta T, Koga H, Horie M, Nakamura T, Otabe K, Nakagawa Y, Katakura M, Sekiya I..
In-vivo kinematics of high-ﬂex posterior-stabilized total knee prosthesis designed for Asian populations.
Int Orthop. 2016.11; 40(11); 2295-2302
15. Kaori Nakamura, Hideyuki Koga, Ichiro Sekiya, Toshifumi Watanabe, Tomoyuki Mochizuki, Masafumi
Horie, Tomomasa Nakamura, Koji Otabe, Takeshi Muneta. Dynamic Evaluation of Pivot-Shift Phenomenon in Double-Bundle Anterior Cruciate Ligament Reconstruction Using Triaxial Accelerometer.
Arthroscopy. 2016.12; 32(12); 2532-2538
16. Inoue M, Muneta T, Ojima M, Nakamura K, Koga H, Sekiya I, Okazaki M, Tsuji K.. Inﬂammatory
cytokine levels in synovial ﬂuid 3, 4 days postoperatively and its correlation with early-phase functional
recovery after anterior cruciate ligament reconstruction: a cohort study. J Exp Orthop.. 2016.12; 3(1);30― 429 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

17. Toshiyuki Ohara, Takeshi Muneta, Yusuke Nakagawa, Yu Matsukura, Shizuko Ichinose, Hideyuki Koga,
Kunikazu Tsuji, Ichiro Sekiya. < Original Article> Hypoxia enhances proliferation through increase ofcolony formation rate with chondrogenic potential in primary synovial mesenchymal stem cells. J. Med.
Dent. Sci.. 2016.12; 63(4); 61-70[Conference Activities & Talks]
1. Yusuke Nakagawa, Hideyuki Koga, Takeshi Muneta, Kiuchi S, Ono H, Nagata T, Toshifumi Watanabe,
Masafumi Horie, Tomomasa Nakamura, Koji Otabe, Mai Katakura, Hisako Katano, Ichiro Sekiya. Threedimensional Reconstructed MRI Evaluation of Arthroscopic Centralization Technique for Extruded Lateral
Meniscus. ORS 2016 2016.03.05
2. Koji Otabe, Ken Watanabe, Norio Shimizu, Takeshi Muneta, Ichiro Sekiya. High Sensitivity Virus /Mycoplasma Screening Test Reveals High Prevalence of Parvovirus B19 Infection In Synovial Tissues andBone Marrow. Orthopaedic Research Society 2016 Annual Meeting 2016.03.06 Orlando, USA
3. Mitsuru Mizuno, Hisako Katano, Koji Otabe, Keiichiro Komori, Yukie Matsumoto,Nobutake Ozeki, Kunikazu Tsuji, Takeshi Muneta, Ichiro Sekiya. PDGF-AA/AB In Human Serum Are Potential Indicators Of
The Proliferative Capacity Of Human Synovial Mesenchymal Stem Cells. Orthopaedic Research Society
2016 Annual Meeting 2016.03.06 Orlando, USA
4. Mitsuru Mizuno, Hisako Katano, Yo Mabuchi, Yusuke Ogata, Shizuko Ichinose, Koji Otabe, Keiichiro
Komori, Yukie Matsumoto, Nobutake Ozeki, Kunikazu Tsuji, Chihiro Akazawa, Takeshi Muneta, Ichiro
Sekiya. Properties of MSCs Derived From Surface, Stroma, and Perivascular Synovium Of Oa Patients.
Orthopaedic Research Society 2016 Annual Meeting 2016.03.06 Orlando, USA
5. Kenta Katagiri, Takeshi Muneta, Koji Otabe, Yu Matsukura, Hisako Katano,Ichiro Sekiya. Fibrous
Synovium Releases Higher Number Of MSCs Than Adipose Synovium in a Suspended Synovium Culture
Model. Orthopaedic Research Society 2016 Annual Meeting 2016.03.06 Orlando, USA
6. Mana Naritomi, Mitsuru Mizuno, Hisako Katano, Koji Otabe, Nobutake Ozeki, Kunikazu Tsuji, Takeshi
Muneta, Ichiro Sekiya. Interleukin-1 β Promotes In Vitro Chondrogenesis Of Synovial Mesenchymal StemCells. Orthopaedic Research Society 2016 Annual Meeting 2016.03.06 Orlando, USA
7. Nobutake Ozeki, Junpei Matsuda, Takeshi Muneta, Hideyuki Koga, Kunikazu Tsuji, Kenta Katagiri,
Mitsuru Mizuno, Tomoyuki Saito, Ichiro Sekiya. Biomechanical Analysis of Centralization With an Anchor
for Meniscus Extrusion in a Porcine Model. Orthopaedic Research Society 2016 Annual Meeting 2016.03.07
Orlando, USA
8. Yusuke Nakagawa, Hideyuki Koga, Takeshi Muneta, Shinji Kiuchi, Hideo Ono, Tsuyoshi Nagata, Toshifumi Watanabe, Masafumi Horie, Masafumi Horie, Tomomasa Nakamura, Koji Otabe, Mai Katakura,
Hisako Katano, Ichiro Sekiya. Three-Dimensional Reconstructed MRI Evaluation of Arthroscopic Centralization Technique for Extruded Lateral Meniscus. Orthopaedic Research Society 2016 Annual Meeting2016.03.07 Orlando, USA
9. Ichiro Sekiya. Adult stem cell-based approaches (Intraarticular delivery, model systems vs humans)
Overview. ICRS Summit 2016.04.10 Kyoto, JAPAN10. Ichiro Sekiya. Scientiﬁc update: the synovial joint milieu - implications for cartilage repair. ICRS World
Congress 2016.09.23 Naples,Italy[Awards & Honors]
1. Ichiro Sekiya, The Japanese Society for Regenerative Medicine Award (Clinical Category), The JapaneseSociety for Regenerative Medicine, 2016.03
2. Koji Otabe, Excellence in Translational Science Award from Journal of Orthopaedic Research, Orthopaedic
Research Society, 2016.03
3. Mitsuru Mizuno, Young Investigator Award, The Japanese Tissue Culture Association, 2016.05
4. Yusuke Nakagawa, Grant for Study Abroad by Mitsukoshi Health and Welfare Foundation, Mitsukoshi
Health and Welfare Foundation, 2016.08― 430 ―

Advanced Therapeutic Sciences

JFCR Cancer Biology

Professor

Takuro NAKAMURA

Professor Kiyotaka SHIBA
Professor Akihiro TOMIDA
Professor Toru HIROTA
Associate Professor Kengo TAKEUCHI
Graduate Students Yuki TOGASHI, Yui
SAKAIDA(1) Research
Understanding the mechanisms of carcinogenesis and cancer progression. Studying thebasics of personalized medicine for innovative cancer therapy.

(2) Education
We are committed to training talented and motivated graduate students, helping
launch careers in basic and translational cancer research.
1. Molecular mechanisms of carcinogenesis and identification of cell-of-origin of cancer(Nakamura)
2. Pathological and genetic analysis of human cancer such as malignant lymphoma andlung cancer (Takeuchi)
3. Application of nanobiotechnology in cancer diagnostics (Shiba)
4. Strategy for innovative drug therapy based on cancer biology (Tomida)
5. Signaling pathways that induce cellular senescence in vivo and to elucidate howthese pathways are perturbed in cancer cells
6. Understanding mitotic chromosome dynamics in cancer, to exploit mitotic control tocancer intervention (Hirota)(3) Publications[Original Articles]
1. Iwai, K., Minamisawa, T., Suga, K., Yajima, Y., Shiba, K. Isolation of human salivary
extracellular vesicles by iodixanol density gradient ultracentrifugation and theircharacterizations. J Extracell Vesicles 5, 30829, 2016.
2. Tsukumo, Y., Tsukahara, S., Furuno, A., Iemura, S., Natsume, T., Tomida, A. TBL2
associates with ATF4 mRNA via its WD40 domain and regulates its translation― 431 ―

Advanced Therapeutic Sciences

during ER stress. J Cell Biochem, 117:500-509, 2016.
3. Koido, M., Sakurai, J., Tsukahara, S., Tani, Y., Tomida, A. PMEPA1, a TGF-ǃ- andhypoxia-inducible gene that participates in hypoxic gene expression networks insolid tumors. Biochem Biophys Res Commun, 479:615-6214, 2016.
4. Hiratsuka T, Takei Y, Ohmori R, Imai Y, Ozeki M, Tamaki K, Nakamura T, Tsuruyama T.
ZFP521 contributes to pre-B-cell lymphomagenesis through modulation of the pre-B-cell
receptor signaling pathway. Oncogene, 35:3227-3238, 2016.
5. Yokoyama T, Nakatake M, Kuwata T, Couzinet A, Goitsuka R, Tsutsumi S, Aburatani H, Valk
PJM, Delwel R, Nakamura T. MEIS1-mediated transactivation of synaptotagmin like 1
promotes CXCL12/CXCR4 signaling and leukemogenesis. J Clin Invest, 126:1664-1678, 2016.
6. Tsuruyama T, Hiratsuka T, Aini W, Nakamura T. STAT5A modulates chemokine receptor CCR6
expression and enhances pre-B cell growth in a CCL20-dependent manner. J Cell Biochem,
117:2630-2642, 2016.
7. Minas TZ, Surdez D, Tahereh J, Tanak M, Howarth M, Kang HJ, Han J, Han ZY, Sax B, Kream
BE, Hong SH, Tirode F, Tuckermann J, Toretsky JA, Kenner L, Kovar H, Lee S, Sweet-Cordero
A, Nakamura T, Moriggi R, Delattre O, Uren A. Combined experience of six independent
laboratories

attemptingtocreateanEwing

sarcomamouse

model.

Oncotarget,

DOI:10.18632/oncotarget.9388, 2016.
8. Keeshan K, Vieugue P, Chaudhury S, Rishi L, Gallard C, Liang L, Garcia E, Nakamura T,
Omidvar N, Kogan SC. Cooperative leukaemogenesis in acute myeloid leukaemia and acutepromyelocytic leukaemia reveal C/EBPa as a common target of TRIB1 and PML/RARA.
Haematologica, 101:1228-1236, 2016.
9. Takahashi, M., Wakai, T., Hirota, T. Condensin I-mediated mitotic chromosome assembly
requires association with chromokinesin KIF4A. Genes Dev. 30: 1931-1936, 2016.
10. Nagasaka, K., Hossain, JM., Roberti, JM., *Ellenberg, J., Hirota, T. Sister chromatid resolution
is an intrinsic part of chromosome organization in prophase. Nat Cell Biol. 18: 692-699, 2016.
11. Abe, Y., Sako, K., Takagaki, K., Hirayama, Y., Uchida, KSK., Herman, J., DeLuca, JG., Hirota,
T. HP1-assisted Aurora B kinase activity prevents chromosome segregation errors. Dev. Cell.
36: 487-497, 2016.
12. Abe, Y., Hirota, T. System-level deficiencies in Aurora B control in cancers. Cell Cycle. 10:1-2,
2016.
13. Takahashi, M., Tanaka, K., Wakai, T., Hirota, T. Phosphoproteomic analysis of human mitotic
chromosomes identified a chromokinesin KIF4A. Biomed Res. 37: 161-165, 2016.
14. Harada Y, Koyama T, Takeuchi K, Shoji K, Hoshi K, Oyama Y. NUT midline
carcinoma mimicking a germ cell tumor: a case report. BMC Cancer. 16:895, 2016.
15. Shigematsu Y, Matsuura M, Nishimura N, Tsuyama N, Takeuchi K, Terui Y,― 432 ―

Advanced Therapeutic Sciences

Takeshima N, Hatake K. Intravascular Large B-cell Lymphoma of the Bilateral
Ovaries and Uterus in an Asymptomatic Patient with a t(11;22)(q23;q11)
Constitutional Translocation. Intern Med. 55:3169-3174, 2016.
16. Chang ST, Chen SW, Ho CH, Kuo CC, Sakata S, Takeuchi K, Chuang SS.
Immunophenotypic and genetic characteristics of diffuse large B-cell lymphoma in
Taiwan. J Formos Med Assoc. 115:961-967, 2016.
17. Koyama M, Terauchi T, Koizumi M, Tanaka H, Takeuchi K. Sinonasal oncocytic
Schneiderian papilloma accompanied by intravascular lymphoma: A case report on
FDG-PET/CT imaging. Medicine (Baltimore). 95:e4646, 2016.
18. Kurita D, Takeuchi K, Kobayashi S, Hojo A, Uchino Y, Sakagami M, Ohtake S,
Takahashi H, Miura K, Iriyama N, Sugitani M, Miyoshi H, Hatta Y, Ohshima K,
Takei M. A cyclin D1-negative mantle cell lymphoma with an IGL-CCND2translocation that relapsed with blastoid morphology and aggressive clinicalbehavior. Virchows Arch. 469:471-476, 2016.
19. Kataoka K, Shiraishi Y, Takeda Y, Sakata S, Matsumoto M, Nagano S, Maeda T,
Nagata Y, Kitanaka A, Mizuno S, Tanaka H, Chiba K, Ito S, Watatani Y, Kakiuchi N,
Suzuki H, Yoshizato T, Yoshida K, Sanada M, Itonaga H, Imaizumi Y, Totoki Y,
Munakata W, Nakamura H, Hama N, Shide K, Kubuki Y, Hidaka T, Kameda T,
Masuda K, Minato N, Kashiwase K, Izutsu K, Takaori-Kondo A, Miyazaki Y,
Takahashi S, Shibata T, Kawamoto H, Akatsuka Y, Shimoda K, Takeuchi K, Seya T,
Miyano S, Ogawa S. Aberrant PD-L1 expression through 3'-UTR disruption in
multiple cancers. Nature. 534:402-406, 2016.
20. Katsushima H, Fukuhara N, Ichikawa S, Ota Y, Takeuchi K, Ishizawa K, Sasano H,
Harigae H, Ichinohasama R. Non-biased and complete case registration of lymphoidleukemia and lymphoma for five years: a first representative index of Japan from anepidemiologically stable Miyagi Prefecture. Leuk Lymphoma. 1-9, 2016.
21. Harada A, Oguchi M, Terui Y, Takeuchi K, Igarashi M, Kozuka T, Harada K, Uno T,
Hatake K. Radiation therapy for localized duodenal low-grade follicular lymphoma.
J Radiat Res. 57:412-417, 2016.
22. Inoue M, Toki H, Matsui J, Togashi Y, Dobashi A, Fukumura R, Gondo Y, Minowa O,
Tanaka N, Mori S, Takeuchi K, Noda T. Mouse models for ROS1-fusion-positive lungcancers and their application to the analysis of multikinase inhibitor efficiency.
Carcinogenesis. 37:452-460, 2016.
23. Horiike A, Takeuchi K, Uenami T, Kawano Y, Tanimoto A, Kaburaki K, Tambo Y,
Kudo K, Yanagitani N, Ohyanagi F, Motoi N, Ishikawa Y, Horai T, Nishio M.
Sorafenib treatment for patients with RET fusion-positive non-small cell lung

― 433 ―

Advanced Therapeutic Sciences

cancer. Lung Cancer. 93:43-46, 2016.
24. Katayama R, Sakashita T, Yanagitani N, Ninomiya H, Horiike A, Friboulet L,
Gainor JF, Motoi N, Dobashi A, Sakata S, Tambo Y, Kitazono S, Sato S, Koike S,
John Iafrate A, Mino-Kenudson M, Ishikawa Y, Shaw AT, Engelman JA, Takeuchi K,
Nishio M, Fujita N. P-glycoprotein Mediates Ceritinib Resistance in Anaplastic
Lymphoma Kinase-rearranged Non-small Cell Lung Cancer. EBioMedicine. 3:54-66,
2016.
25. Kusano H, Togashi Y, Akiba J, Moriya F, Baba K, Matsuzaki N, Yuba Y, Shiraishi Y,
Kanamaru H, Kuroda N, Sakata S, Takeuchi K, Yano H. Two Cases of Renal Cell
Carcinoma Harboring a Novel STRN-ALK Fusion Gene. Am J Surg Pathol.
40:761-769, 2016.
26. Sakamoto K, Nakasone H, Togashi Y, Sakata S, Tsuyama N, Baba S, Dobashi A,
Asaka R, Tsai CC, Chuang SS, Izutsu K, Kanda Y, Takeuchi K. ALK-positive large
B-cell lymphoma: identification of EML4-ALK and a review of the literature
focusing on the ALK immunohistochemical staining pattern. Int J Hematol.
103:399-408, 2016.
27. Dobashi A, Tsuyama N, Asaka R, Togashi Y, Ueda K, Sakata S, Baba S, Sakamoto K,
Hatake K, Takeuchi K. Frequent BCOR aberrations in extranodal NK/T-Celllymphoma, nasal type. Genes Chromosomes Cancer. 55:460-471, 2016.
28. Takeuchi K, Togashi Y, Kamihara Y, Fukuyama T, Yoshioka H, Inoue A, Katsuki H,
Kiura K, Nakagawa K, Seto T, Maemondo M, Hida T, Harada M, Ohe Y, Nogami N,
Yamamoto N, Nishio M, Tamura T. Prospective and clinical validation of ALK
immunohistochemistry: results from the phase I/II study of alectinib for
ALK-positive lung cancer (AF-001JP study). Ann Oncol. 27:185-192, 2016.

[Review Articles]
1. Tanaka, K., Hirota, T. Chromosomal instability: a common feature and atherapeutic target of cancer. Review. Biochim Biophys Acta. 1866: 64-75, 2016.
[Conference Activities & Talks]
1. Shiba K.

Differentiation of extracellular vesicles using aptamer functionalized

substrate surface.

XXV International Materials Research Congress 2016

(Cancun) August, 2016
2. Nakamura T. Signaling and transcriptional networks in myeloid leukemogenesis. The 5th
JCA-AACR Special Joint Conference (Urayasu) July, 2016
3. Nakamura T. Modeling soft part sarcoma to clarify interaction between sarcoma cells and― 434 ―

Advanced Therapeutic Sciences

microenvironment. The JSA and JSCS Joint Annual Meeting 2016 (Kyoto) December, 2016
4. Hirota, T. HP1-mediated allosteric regulation of Aurora B prevents chromosome segregation
errors. International symposium on chromosome orchestration system.㸦Awaji㸧March, 2016
5. Hirota, T. Kinetic control of the M/A transition in failsafe mitosis. SKKU International
symposium on biomedical science. (Suwon) October, 2016

― 435 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

Minimally Invasive Medical Treatment

Professor KOJIMA Kazuyuki(1)

Outline1. Investigation and research for the social needs identiﬁcation of minimally invasive medical treatment in thenext generation of the medical and the dental areas.
2. Medical equipment and development of treatment to meet the needs of minimally invasive medical treatmentin the next generation of the medical and the dental ﬁelds.
3. Research and development of the education curriculum and evaluation methods of minimally invasive treatment in the medical and the dental ﬁelds.
4. Development and operation of minimally invasive treatment of industry-academia cooperation in the medical
and the dental ﬁelds.
5. Development and operation of technology certiﬁcation strategy of minimally invasive treatment in the medical
and the dental areas.
(2)

Publications[Original Articles]
1. Inokuhi M,Otsuki S,Ogawa N,Tanioka T,Okuno K,Gokita K,Kawano T,Kojima K. Postoperatibe Complications of Laparoscopi Total Gastrectomy versus Open Total Gastrectomy for Gastric Cancer in a
Meta-Analysis of High-Quality Case-Control Studies. Gastroenterology Res Pract.
2. Nishikawaji t,Akiyama Y,Shimada s,Kojima K,Kawano T,Eishi Y,Yusa Y,Tanaka S. Onogogenic roles of
the SETDB2 histone methyltransferase in gastric cancer. Onocotaeget.
3. Higuchi K,Inokuchi M,Takagi Y,Ishikawa T,Otsuki S,Uetake H,Kojika K,Kaawano T. Cadherin 5 expression correlates with poor survival in human gastric cancer. J Clin Pathol.
4. Nakagawa M, Kojima K,Inokuchi M,Kato K,Sugita H,Otsuki S,Sugihara K. Identiﬁcation of frequency,
severity and risk factors of complications after open gastrectomy: Retrospective analysis prospectively
collected database using the Clavien-Dindo classiﬁcation. J Med Dent Sci.
5. Yamada I, Hikishima K, Miyasaka N, Kato K, Ito E, Kojima K, Kawano T, Kobayashi D, Eishi Y, Okano
H. q-Space MR Imaging of Gastric Carcinoma Ex Vivo: Correlation with Histopathologic Findings. Magn
Reson Med.
6. Inokuchi M, Otsuki S, Murase H, Kojima K, Kawano T. Feasibility of laparoscopy-assisted gastrectomy
for patients with poor physical status: A propensity-score matching study. Int J Surg.
7. Akiyama Y, Koda Y, Byeon SJ, Shimada S, Nishikawaji T, Sakamoto A, Chen Y, Kojima K, Kawano
T, Eishi Y, Deng D, Kim WH, Zhu WG, Yuasa Y, Tanaka S. Reduced expression of SET7/9, a histone
mono-methyltransferase, is associated with fastric cancer progression. Oncotarget.
8. Kobayashi k,Inokuchi M, Takagi Y,Otsuki S,Fujimori Y,Sato Y,Yanaka Y,Higuchi K,Aburatani T,Tomii
C,Uketake H,Kojima K,Kawano T. Prognostic signiﬁcance of PAK4 expression in gastric cancer. J Clin
Pathol.

― 436 ―

Advanced Therapeutic Sciences

Advanced Therapeutic Sciences

9. Yamada I, Hikishima K, Miyasaka N, Kato K, Kojima K, Kawano T, Ito E, Kobayashi D, Eishi Y, Okano
H. Gastric carcinoma: evaluation with diﬀusion-tensor MR imaging and tractography ex vivo. Magnetic
Resonance Imaging.
[Conference Activities & Talks]
1. Kazuyuki Kojima. Laparoscopic Proximal Gastrectomy with Double Tract Reconstruction under 3D
Laparoscopy. The New Golden Standard:3D Laparoscopic Gastric Surgery 2016.08.06 Pusan
2. Kazuyuki Kojima. Learning Pathway to Transition from Open to Lap, speaker. Gastric Inovation Forum2016.10.14 Seoul
3. Kazuyuki Kojima. Laparoscopic reconstruction using circular stapler, speaker. Yonsei Gastric Cancer
Symposium 2016.10.15 Seoul― 437 ―

Life Science and Technology

Life Science and Technology

Biomedical Devices and Instrumentation

Professor: Kohji Mitsubayashi
Junior Associate Professor: Takahiro Arakawa
Assistant Professor: Koji Toma
Lecturer (part-time): Kazuyoshi Yano
Postdoctoral Researcher: Kumiko Miyajima(1)

OutlineOur research is based on a broad range of areas such as electrochemistry, mechanical engineering, electricalengineering, material science and biochemistry. The group aims to pursue interdisciplinary research in bioMEMS, bio-optics, bio-electronics or bioinformatics by combining biotechnology and information technology.
(2)

Research

1. Detachable Cavitas sensors as bioinformation monitoring systems in body cavities"Cavitas sensors" such as a soft contact-lens biosensor and a mouth guard biosensor have been developed fornovel biomonitoring methods by using advanced polymer microelectromechanical systems (MEMS) techniques.
2. Biochemical gas sensor "Bio-sniﬀers" and spatiotemporal gas visualization system Sniﬀ-camera for volatileorganic compounds from human body
Highly selective gas sensors "Bio-sniﬀers" and gas visualization systems "Sniﬀ-camera" for acquiring spatiotemporal information of distribution of volatile chemicals have been developed by exploiting metabolizing enzymes
in human liver. Potential applications of these gas sensors include halitosis analysis, breath alcohol and aldehyde
measurement, medical screening or dental health, etc.
3. Immunosensors for medical treatment and environmental medicine
Development of optical or surface acoustic wave immunosensors have been pursued for semi-continuos (rapid
and repeated) measurement of antigens in body and airborne allergens in living environment.
4. Organic engine based on chemo-mechanical energy conversion
A novel chemo-mechanical energy conversion system (organic engine) that utilizes enzyme reactions and active
transport of chemicals have been constructed. Biomedical applications (chemical pumps, drug release systems,etc.) are also investigated.

(3)

Education

In advanced medicine, technologies enabling to accurately measure biological information are highly demanded.
The development of human-friendly non-invasive measurement methods could release patients from the pain
and the risks of sampling. The students will learn the basic knowledge and skills of biological information measurement through the lectures (e.g., Biomedical Device Science and Engineering , Practice in Global Linkage
between University and Industry and Nanobiotechnology ), seminars and practical training. Especiallyresearch including biochemical measurement, the development of biosensing devices and their applications to
medicine will be carried out based on sensor and biomedical engineering.

― 438 ―

Life Science and Technology

Medical Devices(4)

Lectures & Courses

The students will learn the basic technology related to advanced medicine and biological information measurement. Through practical training, they will also engage in research activities for biochemical measurement,
the development of biosensing devices and their applications to medicine based on sensor and biomedical
engineering . The objective of this course is to help the students be able to think about and conduct a researchby themselves throughout the activities with academic researches.
(5)

Publications[Original Articles]
1. Koji Toma, Kumiko Miyajima, Shin-ichi Sawada, Takahiro Arakawa, Hiroyuki Kudo, Kazunari Akiyoshi,
Kohji Mitsubayashi. Direct measurement of gaseous formaldehyde from food with a ﬁber-optic biochemicalgas sensor (bio-sniﬀer) Sensors and Materials. 2016; 28(12); 1265-1272
2. Po-Jen Chien, Ming Ye, Takuma Suzuki, Koji Toma, Takahiro Arakawa, Yasuhiko Iwasaki, Kohji Mitsubayashi. Optical isopropanol biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH)
Talanta. 2016; 159; 418-424
3. Takahiro Arakawa, Yusuke Kuroki, Hiroki Nitta, Prem Chouhan, Koji Toma, Shin-ichi Sawada, Shuhei
Takeuchi, Toshiaki Sekita, Kazunari Akiyoshi, Shunsuke Minakuchi, Kohji Mitsubayashi. Mouthguard
biosensor with telemetry system for monitoring of saliva glucose: A novel cavitas sensor Biosensors andBioelectronics. 2016; 84; 106-111
4. Hirokazu Saito, Yuki Hashimoto, Takeshi Minamide, Takuo Kon, Koji Toma, Takahiro Arakawa, andKohji Mitsubayashi. Fiber optic bio-sniﬀer (biochemical gas sensor) for gaseous dimethyl sulﬁde Sensors
and Materials. 2016; 28(12); 1295-1301
5. Koji Toma, Yurika Suzuki, Mana Saito, Kumiko Miyajima, Takahiro Arakawa, Hiroji Shimomura, Kohji Mitsubayashi. Fiber-optic chemiﬂuorescent immunosensor for inﬂuenza virus monitoring Journal of
Analytical Bio-Science. 2016; 39(4); 271-276
6. Takahiro Arakawa, Kenta Iitani, Toshiyuki Sato, Koji Toma, Kohji Mitsubayashi. Ethanol vapor imaging
system sniﬀer camera for evaluation of alcohol metabolism from breath and palm skin gas Procedia
Engineering. 2016; 168; 522-528[Misc]
1. Takahiro Arakawa, Kohji Mitsubayashi. Cavitas sensors: Contact lens type sensors & mouthguard sensors
Electroanalysis. 2016; 28; 1170-1187[Conference Activities & Talks]
1. Toma K, Suzuki Y, Saito M, Miyajima K, Arakawa T, Shimomura H, Mitsubayashi K. A chemi-ﬂuorescence
immunosensor with disposable PMMA ﬁber for detection of inﬂuenza viruses. EUROPT(R)ODE XIII
2016.03.20 Graz, Austria
2. Toma K, Arakawa T, Mitsubayashi K. Advanced immunosensors for health care and clinical medicine.
EPFL, Tokyo Tech, TMDU Joint Workshop 2016.04.19 Tokyo, Japan
3. Arakawa T, Iitani K, Sato T, Toma K, Mitsubayashi K. 2D ﬂuorometric sniﬀ-cam with ADH immobilized
mesh and UV-LED sheet for gaseous ethanol imaging from a palm skin. Biosensors 2016 2016.05.25
Gothenburg, Sweden
4. Arakawa T, Kuroki Y, Nitta H, Toma K, Sawada S, Takeuchi S, Sekita T, Akiyoshi K, Minakuchi S,
Mitsubayashi K. A wireless (BLE) mouthguard-biosensor with dental materials for real-time measurementof saliva glucose in oral cavity. Biosensors 2016 2016.05.25 Gothenburg, Sweden
5. Toma K, Miki D, Kishikawa C, Yoshimura N, Miyajima K, Arakawa T, Yatsuda H, Mitsubayashi K. A
surface acoustic wave (SAW) immunosensor with a regeneratable surface enabling repetitive measurementof dust mite allergens. Biosensors 2016 2016.05.25 Gothenburg, Sweden― 439 ―

Life Science and Technology

Medical Devices

6. Ye M, Chien PJ, Suzuki T, Toma K, Sawada S, Arakawa T, Akiyoshi K, Mitsubayashi K. Acetone biosniﬀer(gas-phase biosensor) by detecting NADH consumption on S-ADH reverse reaction for real-time breath
monitoring. Biosensors 2016 2016.05.25 Gothenburg, Sweden
7. Mitsubayashi K. Cavitas (cavity) sensors and Imaging system of human volatiles as non-invasive approaches. Biotechnology and Biochemical Engineering Society of Taiwan (BEST) 2016.06.24
8. Iitani K, Naisierding M, Sato T, Toma K, Arakawa T, Mitsubayashi K. Sniﬀ-cam (gaseous chemical
imaging system) for breath acetaldehyde as a metabolite of ethanol after drinking. APCOT2016 2016.06.26
Kanazawa, Japan
9. Chien PJ, Ye M, Suzuki T, Toma K, Arakawa T, Mitsubayashi K. Gas phase biosensor bio-sniﬀer
acetone measurement and exhaled breath analysis. APCOT2016 2016.06.26 Kanazawa, Japanfor10. Iitani K, Naisierding M, Sato T, Toma K, Arakawa T, Mitsubayashi K. Gas-phase imaging system sniﬀcamera using enzyme mesh for breath acetaldehyde after drinking. IMCS2016 2016.07.10 Jeju, Korea
11. Toma K, Miki D, Yoshimura N, Miyajima K, Arakawa T, Yatsuda H, Mitsubayashi K. A regeneratable
SAW ( Surface Acoustic Wave) immunosensor for repetitive measurement of dust mite allergen. IMCS2016
2016.07.10 Jeju, Korea
12. Kuroki Y, Nitta H, Toma K, Arakawa T, Mitsubayashi K. A mouthguard glucose sensor for monitoringof saliva sugar with ble telemetry system. 2016 IEEE Sixth International Conference on Communications
and Electronics 2016.07.27 Ha Long Bay, Vietnam
13. Arakawa T, Iitani K, Sato T, Naisierding M, Toma K, Mitsubayashi K. Fluorometric imaging system"Sniﬀer-cam" for human breath and skin gas in evaluation of alcohol metabolism. 2016 International
Conference on Optical MEMS and Nanophotonics 2016.07.31 Singapore
14. Arakawa T, Iitani K, Sato T, Toma K, Mitsubayashi K. Ethanol vapor imaging system sniﬀer camera forevaluation of alcohol metabolism from breath and palm skin gas. Eurosensors2016 2016.09.04 Budapest,
Hungary
15. Mitsubayashi K. Cavitas sensors for bio/chemical monitoring: Soft-contact lens & Mouthguard sensors.
2016 International Conference on Flexible and Printed Electronics 2016.09.06
16. Chien P J, Ye M, Suzuki T, Toma K, Arakawa T, Mitsubayashi K. Bio-Sniﬀer (Gas Phase Biosensor) for
Breath Acetone as a Volatile Product of Lipid Metabolism. PRiME 2016 2016.10.02 Honolulu, USA
17. Iitani K, Naisierding M, Sato T, Toma K, Arakawa T, Mitsubayashi K. Sniﬀ-Cam (Bio-Fluorometric
Gas-Imaging System) for Breath Acetaldehyde after Drinking. PRiME 2016 2016.10.02 Honolulu, USA
18. Toma K, Nitta H, Zhang Z, Tomoto K, Shintani Y, Takeuchi S, Arakawa T, Sekita T, Iwasaki Y, Kawarada
H, Minakuchi S, Mitsubayashi K. A Mouthguard Biosensor Using UV Curable Biocompatible Polymer
for Stable Enzyme Immobilization in Saliva Glucose Monitoring. The 1st International Symposium on
Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development(iLIM-1) 2016.10.17 Osaka, Japan
19. Mitsubayashi K. Cavitas sensors and sniﬀ-cam for biomonitoring: Soft contact lens & Mouthguard sensors,
optical bio-sniﬃng of human VOCs. IEEE SENSORS2016 2016.10.30
20. Kishikawa C, Miki D, Miyajima K, Yoshimura N, Yatsuda H, Toma K, Arakawa T, Mitsubayashi K.
SAW immunosensor for on-site monitoring of airborne mite allergen Der f 1 in residential environment.
International Symposium on Biomedical Engineering 2016.11.10 Tokyo, Japan
21. Arakawa T, Mitsubayashi K. Mouthguard and soft contact lens biosensors for non-invasive cavitasmonitoring. International Symposium on Biomedical Engineering, 2016.11.10
22. Chien P J, Ye M, Suzuki T, Tsujii M, Toma K, Arakawa T, Mitsubayashi K. Fluorometric acetone biosniﬀer enabling breath analysis for non-invasive assessment of lipid metabolism. ISLS2016 2016.11.22
Taipei, Taiwan
23. Toma K, Horibe M, Kishikawa C, Yoshimura N, Arakawa T, Yatsuda H, Shimomura H, Mitsubayashi
K. Rapid measurement of mite allergens by a surface acoustic wave (SAW) immunosensor for airborne
allergen monitoring. Bio4Apps2016 2016.12.14 Gold coast, Australia― 440 ―

Life
LifeScience
Science andand Technology
Technology

Biomedical Information

Professor Yuji Miyahara (Concurrent)
Associate Professor Fumimasa NOMURA
Graduate Student
Masao ODAKA
Yoshihiro SUGIO(1)

Research

Studies on Epigenetic Information Stored Living System using Constitutive Reconstruction Strategy
1) Constructing On-chip Organ Model using Microfabrication-Bio Technology.
2) Constructing Artiﬁcial Cell Model using Liposome Technology.
(2)

Publications[Original Articles]
1. López-Redondo F, Kurokawa J, Nomura F, Kaneko T, Hamada T, Furukawa T, Yasuda K. A distribution
analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes Journal of Pharmacological Sciences. 2016.04; 131(2); 141-145
2. Terazono H, Kim H, Nomura F, Yasuda K. Development of a microprocessing-assisted cell-systematic
evolution of ligands by exponential enrichment method for human umbilical vein endothelial cells Japanese
Journal of Applied Physics. 2016.05; 55; 06GN03
3. Nomura F, Hattori A, Terazono H, Kim H, Odaka M, Sugio Y, Yasuda K. Predictive lethal proarrhythmic
risk evaluation using a closed-loop-circuit cell network with human induced pluripotent stem cells derived
cardiomyocytes Japanese Journal of Applied Physics. 2016.05; 55; 06GN07[Conference Activities & Talks]
1. Hayashi T, Tokihiro T, Kurihara H, Nomura F, Yasuda K. Community eﬀect of cardiomyocytes in beating
rhythms is ruled by stable cells. 2016.11.25― 441 ―

Life Science and Technology

Life Science and Technology

Bioelectronics

Staﬀ
Yuji Miyahara (Professor)
Akira Matsumoto (Associate Professor)
Tatsuro Goda (Assistant Professor)
Miyuki Tabata (JSPS Research Fellow)
Yukichi Horiguchi (Project Assistant Professor)
Yoshizumi Toshihiro (Project Assistant Professor)
Taiki Miyazawa (Project Assistant Professor)
Hiroko Matsumoto (Assoiate Research Scientist)
Yuki Morooka (Assoiate Research Scientist)
Chiharu Mizoi (Assoiate Research Scientist)
Sayo Kotaki (Assoiate Research Scientist)
Kayoko Nakagawa (Oﬃce Staﬀ)
Graduate student
Yuki Imaizumi,Hai Wenfeng, Masahiro Touya,Tomoko Sakamaki
Chindanai RATANAPORNCHAROEN, Dilinaer AINIWAER, Hiroaki Hatano(1)

OutlineBioelectronics group is engaged in developing methodologies to determine and analyze functions of biomolecules
and their relationships to diseases based on solid-state biosensor technology. Our interests include design &understanding of physicochemical properties of the interface between biomolecules and the device materials,
signal-transduction mechanism as well as the pursuit of improved sensitivity and selectivity. These technologies
involve many diﬀerent disciplines of science and engineering, through which we propose new solutions to future
medicine.
(2)

Research

1. Bioelectronics for Next-generation DNA Sequencing
Our research is focused on the development of nano-interfaces between biomolecules and semiconducting materials for label-free and highly sensitive electrical monitoring of nucleotide base sequences and their ampliﬁcation
processes. The goal of the project is to provide a smaller and cheaper alternative next-generation DNA sequencer to the traditional techniques that involve optical sensing using ﬂuorescence and bioluminescence.
2. Devices for Early Cancer Diagnosis
For applications to early-stage diagnostics of cancers, we aim to establish the device technology enabling detection of small amount of cancer markers out of blood samples with remarkable quickness and sensitivity.
The focus is on the design of nano-interfaces that involves chemical modiﬁcation of biomolecular targets aswell as solid/ liquid interfaces in order to achieve eﬃcient biomolecular recognitions on the electrode surfaces.
We also pursue optimized materials and the surface property of the electrode in order to obtain remarkably
target-speciﬁc signals out of complicated electrical signals obtained from raw biological samples.
3. Discovering Intra/Extracellular Molecular Dynamics on Inﬂammatory Response
Molecular dynamics at inﬂammation and bacterial infection is investigated using biomimetic surfaces. The term
biomimetics in this context represents mimicking the interplay between biomolecules and local changes of― 442 ―

Life Science and Technology

Life Science and Technology

microenvironment that has evolved as a mechanism for inauguration of immune responses. Our new nano-biotechnology will reveal unidentiﬁed active molecular dynamics in pathophysiology.
4. Artiﬁcial Pancreas to Treat Diabetes
Development of self-regulated insulin delivery systems to treat diabetes is a long-standing challenge of biomedical
engineering. We propose a synthetic gel based solution, which could oﬀer a remarkably simple, electronicsfree and thus signiﬁcantly low-cost alternative to the ongoing eﬀorts of artiﬁcial pancreas.

(3)

Education

1. Engagement: we are engaged in teaching a part of Biomedical Engineering course and mentoring master &doctor students.
2. Course objective: Serum components play crucial roles in metabolic cycles and their concentration homeostasis reﬂects dynamic equilibrium of life. On occasion of abnormal metabolic pathway, it is manifested asa ﬂuctuation of each speciﬁc serum component. Our lecture provides an overview of advanced materials andengineering aimed at determination of body ﬂuids including serum components and mechanisms for their concentration homeostasis.
3. Deepen knowledge of theory, mechanisms, methodologies, application, and limitation of detection technology for biomolecules in various clinical samples. Learn integrative technology of advanced materials/devices
and biology/medicine, present problems and future perspective in bioelectronics. Familiarize each student withother related techniques, lab skills including planning of experiments, presenting research results and preparing
reports.

(4)

Publications[Original Articles]
1. Akira Matsumoto, Mai Yuasa, Hiroko Matsumoto, Mai Sanjo, Miyuki Tabata, Tatsuro Goda, Toru Hoshi,
Takao Aoyagi, Yuji Miyahara. Boronate Functionalized Polymer Gel Based Insulin Delivery System with
Improved Stability in Performance: A Comparative Structure-Function Study 2016.02; 45(4); 460-462
2. Tatsuro Goda, Eriko Yamada, Yurika Katayama, Miyuki Tabata, Akira Matsumoto, Yuji Miyahara.
Potentiometric responses of ion-selective microelectrode with bovine serum albumin adsorption Biosensors
and Bioelectronics. 2016.03; 77; 208-214
3. Ayaka Seichi, Nanami Kozuka, Yuko Kashima, Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Naoko
Iwasawa, Daniel Citterio, Yuji Miyahara, Koji Suzuki. Real-time Monitoring and Detection of Primer
Generation-Rolling Circle Ampliﬁcation of DNA using an Ethidium Ion-Selective Electrode Anal. Sci..
2016.03; 32(5); 505-510
4. Daniel Schaﬀhauser, Michael Fine, Miyuki Tabata, Tatsuro Goda, Yuji Miyahara. Measurement of Rapid
Amiloride-Dependent pH Changes at the Cell Surface Using a Proton-Sensitive Field-Eﬀect Transistor
Biosensors. 2016.03; 6(2); 11
5. Yuki Imaizumi, Tatsuro Goda, Yutaro Toya, Akira Matsumoto, Yuichi Miyahara. Oleyl Group-functionalized
Insulating Gate Transistors for Measuring Extracellular pH of Floating Cells Sci. Technol. Adv. Mater..
2016.07; 17(1); 337-345
6. Tatsuro Goda, Yuji Miyahara. Engineered Zwitterionic Phosphorylcholine Monolayers for Elucidating
Multivalent Binding Kinetics of C-reactive Protein Acta Biomater.. 2016.08; 40; 46-53
7. Zhenyu Gao, Yukichi Horiguchi, Kei Nakai, Akira Matsumura, Minoru Suzuki, Koji Ono, Yukio Nagasaki.
Use of boron cluster-containing redox nanoparticles with ROS scavenging ability in boron neutron capture
therapy to achieve high therapeutic eﬃciency and low adverse eﬀects Biomaterials. 2016.10; 104; 201-212
8. Miyuki Tabata, Chindanai Ratanaporncharoen, Aoi Asano, Yuichi Kitasako, Masaomi Ikeda, TatsuroGoda, Akira Matsumoto, Junji Tagami, Yuji Miyahara. Miniaturized Ir/IrOx pH Sensor for Quantitative
Diagnosis of Dental Caries Procedia Eng.. 2016.11; 168; 598-601― 443 ―

Life Science and Technology

Life Science and Technology

9. Miyuki Tabata, Yurika Katayama, Fahmida Mannan, Ayaka Seichi, Koji Suzuki, Tatsuro Goda, Akira
Matsumoto, Yuji Miyahara. Label-free and Electrochemial Detection of Nucleic Acids Based on Isothermal
Ampliﬁcation in Combination with Solid-state pH Sensor Procedia Eng.. 2016.11; 168; 419-422[Books etc]
1. Yukichi Horiguchi, Yukio Nagasaki. Encyclopedia of Biocolloid and Biointerface Science. John Wiley &Sons, Inc., 2016.06 (ISBN : 978-1-1185-4276-7)[Misc]
1. Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Yuji Miyahara. Field-Eﬀect Transistors for Detectionof Biomolecular Recognition Intelligent Nanosystems for Energy, Information and Biological Technologies.
2016.09; 13-25
2. Tatsuro Goda. Sensing Cellular Microenvironments Using Ion-sensitive Field-eﬀect Transistors 2016.10;34(3); 204-209
3. Tatsuro Goda. Sensing Nanomaterials-induced Cytotoxicity and Nanomedicine Chemical Industry. 2016.11;
67(11); 834-844[Conference Activities & Talks]
1. Goda T, Miyahara Y. Conducting Polymers Bearing Phosphorylcholine Group for Metal-free, Antibodyfree, and Low-impedance Biosensing of C-reactive Protein. 7th Japan-Taiwan Nanomedicine Symposium2016.01.21 Kyoto, Japan
2. Matsumoto A, Miyahara Y. Borono-lectin based Platforms for Bio-sensing and Drug Delivery Applications. The 33rd International Conference on Photopolymer Science and Technology 2016.01.24 Chiba,Japan
3. Matsumoto A. Phenylboronate based Strategies for Drug Delivery Systems and Nanobiosensing. 8th International Symposium on Advanced Plasma Science and its Applications for Nitrides and Nanomaterials/9th
International Conference on Plasma-Nano Technology & Science (ISPlasma2016 / IC-PLANTS2016)2016.03.06 Higashiyama Campus, Nagoya University, Aichi, Japan
4. Ratanaporncharoen C, Tabata M, Kitasako Y, Ikeda M, Tagami J, Goda T, Matsumoto A, Miyahara Y.
Fabrication of Micro Ir/IrOx pH Sensor for Dental Applications. PITTCON 2016 2016.03.06 Atlanta,
Georgia, USA
5. Matsumoto A. Synthetic Gel based Approaches towards Electronics-freeTokyo Tech, TMDU Joint Workshop 2016.04.19 TMDU, Japan

Artiﬁcial Pancreas. EPFL,

6. Horiguchi Y, Goda T, Matsumoto A, Takeuchi H, Yamaoka S, Miyahara Y. Rapid and Label-free Inﬂuenza Virus Detection with Densely Immobilized Sialic Acids on Gold Electrode. ImPACT Mitata PM
International Symposium on InSECT 2016 2016.04.26 Nagoya, Japan
7. Yoshizumi T, Goda T, Matsumoto A, Miyahara Y. Development Progress of a FET Type Gas Sensor
Consisting of Gas-Sensitive Polymer and Porous Gate. ImPACT International Symposium on InSECT
2016 2016.04.26 Nagoya, Japan
8. Goda T, Miyahara Y. Organic bioelectronics create ionic microenvironments of inﬂammation and infection
on phospholipid polymer to elucidate molecular dynamics of C-reactive protein. 10th World Biomaterials
Congress (WBC 2016) 2016.05.17 Montreal, QC, Canada
9. Goda T, Miyahara Y. Cells-Attached pH-Sensing Transistor System Detects Proton-Permeable Nanoporeson Plasma Membranes by Exogenous Chemical Compounds. Biosensors 2016 2016.05.25 Gothenburg,
Sweden10. Ratanaporncharoen C, Tabata M, Kitasako Y, Ikeda M, Tagami J, Goda T, Matsumoto A, Miyahara Y.
Fabrication of Ir/IrOx Micro pH Sensor for Quantitative Dental Caries Evaluation. Asia-Paciﬁc Conference of Transducers and Micro-Nano Technology 2016 (APCOT 2016) 2016.06.26 Kanazawa, Japan― 444 ―

Life Science and Technology

Life Science and Technology

11. Goda T. Evaluation of plasma membrane leakage using protons. 2nd International Symposium on
Nanoarchtectonics for Mechanobiology 2016.07.27 Tsukuba, Ibaraki, Japan
12. Matsumoto A. Takayoshi Suganami, Electronics-free and Symthetic Materials Based Approach toward
Artiﬁcial Pancreas. Seminar at City of Hope 2016.08.23 Pasadena, USA
13. Goda T, Toya M, Hai W, Matsumoto A, Miyahara Y. Functional poly(3,4-ethylenedioxythiophenes) forbioanalytics and diagnostics. Biointerfaces International 2016 2016.08.23 University of Zurich, Zurich,
Switzerland
14. Tabata M, Ratanaporncharoen C, Asano A, Kitasako Y, Ikeda M, Goda T, Matsumoto A, Tagami J,
Miyahara Y. Miniaturized Ir/IrOx pH sensor for quantitative diagnosis of dental caries. Eurosensors2016
2016.09.04 Budapest, Hungary
15. Tabata M, Katayama Y, Mannan F, Seichi A, Suzuki K, Goda T, Matsumoto A, Miyahara Y. Labelfree and electrochemial detection of nucleic acids based on isothermal ampliﬁcation in combination withsolid-state pH sensor. Eurosensors2016 2016.09.04 Budapest, Hungary
16. Tabata M, Katayama Y, Mannan F, Seichi A, Suzuki K, Goda T, Matsumoto A, Miyahara Y. Labelfree and electrochemical detection of nucleic acids based on isothermal ampliﬁcation in combination withsolid-state pH sensor. Eurosensors2016 2016.09.04 Budapest, Hungary
17. Goda T, Toya M, Matsumoto A, Miyahara Y. Zwitterionic phosphorylcholine^tethered polythiophenes
for electrochemical detection of acute-phase biomarker. International Conference on Flexible and Printed
Electronics (ICFPE) 2016 2016.09.06 Yonezawa, Yamagata, Japan
18. Hai W, Horiguchi Y, Goda T, Matsumoto A, Takeuchi H, Yamaoka S, Miyahara Y. Label-free and speciﬁc
Human inﬂuenza virus detection using sialyllactose-immobilized gold electrode. International Conferenceon Flexible and Printed Electronics (ICFPE) 2016 2016.09.06 Yonezawa, Yamagata, Japan
19. Sakamaki T, Matsumoto A, Kondo S, Sakai T, Sato S, Kikuchi A, Tei Y, Goda T, Miyahara Y. Demonstration of environment-sensitive tetra-gel by using stimuli-sensitive macromer. The International Symposium on Visualization in Joining & Welding Science through Advanced Measurements and Simulation in
conjunction with Symposium on the Research Activities of Joint Usage/Research Center on Joining and
Welding & The 1st International Symposium on Creation of Life Innovation Materials for Interdisciplinary
and International Researcher Development (iLIM-1) 2016.10.17 Hotel Hankyu Expo Park, Osaka, Japan
20. Ratanaporncharoen C,Tabata M,Ishihara N,Masu K,Sriyudthsak M,Goda T,Matsumoto A,Miyahara Y.
Miniaturized Ir/IrOx wireless pH sensor for bio-sensing system. The 34th Sensor Symposium on Sensors,
Micromachines and Applied Systems 2016 2016.10.24 Hirado Bunka Center, Nagasaki, Japan
21. Horiguchi Y, Goda T, Matsumoto A, Miyahara Y,. Label-free detection of inﬂuenza subtypes based on
multisite binding to sialic acid receptors immobilized gold electrode. 9th International Microprocesses andNanotechnology Conference (MNC 2016) 2016.11.08 Kyoto, Japan
22. Tabata M, Tenaglia E, Goda T, Matsumoto A, Guiducci C, Miyahara Y. pH-based nucleic acid ampliﬁcation detection on a silicon nanowire chip. International Symposium on Biomedical Engineering, 20162016.11.10 Tokyo, Japan
23. Hai W, Goda T, Matsumoto A, Takeuchi H, Yamaoka S, Miyahara Y. Label-free and speciﬁc humaninﬂuenza virus detection using sialyllactose-grafted conducting polymers. International Symposium on
Biomedical Engineering 2016.11.10 Tokyo, Japan
24. Sakamaki T, Matsumoto A, Kondo S, Sakai T, Sato S, Kikuchi A, Tei Y, Goda T, Miyahara Y. Preparation of environment-sensitive tetra-gel by using stimuli-sensitive macromer. International Symposium on
Biomedical Engineering 2016.11.10 Tokyo, Japan
25. Toya M, Goda T, Matsumoto A, Miyahara Y. Poly(3,4-ethylenedioxythiophene) bearing zwitterionic groupfor biosensing application. International Symposium on Biomedical Engineering 2016.11.10 Tokyo, Japan
26. Ratanaporncharoen C, Tabata M, Kitasako Y, Ikeda M, Goda T, Matsumoto A, Tagami J, Miyahara Y.
Fabrication and characterization of Ir/IrOx pH sensor for quantitative dental caries analysis. International
Symposium on Biomedical Engineering 2016.11.10 Tokyo, Japan― 445 ―

Life Science and Technology

Life Science and Technology

27. Miyahara Y. Detection of biomolecular recognition using bio-transistors. International Symposium on
Biomedical Engineering 2016.11.11 Tokyo,Japan
28. Goda T. Application of Zwitterionic Materials: From Infection Biomarker Sensing to Cell Therapy. the18th Takayanagi Kenjiro Memorial Symposium 2016.11.15 Shizuoka University Hamamatsu Campus,
Hamamatsu, Shizuoka, Japan.
29. Matsumoto A, Suganami T, Matsumoto H, Tanaka M, Ochi K, Moro-oka Y, Ogawa Y, Ishii T, Kataoka K,
Miyahara Y. Electronics-free and synthetic polymeric materials based approach toward artiﬁcial pancreas.
3rd International Conference on Biomaterials Science in Tokyo 2016.11.28 Tokyo, Japan
30. Goda T, Imaizumi Y, Hatano H, Matsumoto A, Miyahara Y. Sensing cell barrier functions by proton
dynamics. 3rd International Conference on Biomaterials Science in Tokyo 2016.11.28 Tokyo, Japan
31. Horiguchi Y, Goda T, Matsumoto A, Miyahara Y. Label-free and speciﬁc virus detection using densely immobilized sialic acid receptors. 3rd International Conference on Biomaterials Science in Tokyo 2016.11.28
Tokyo, Japan
32. Miyazawa T, Nakagawa K, Harigae T, Itaya M, Miyazawa T. Eﬀects of encapsulation in poly-(lacticco-glycolic acid) on metabolism of curcumin via oral administration. 3rd International Conference on
Biomaterials Science in Tokyo 2016.11.28 Tokyo, Japan
33. Sakamaki T, Matsumoto A, Kondo S, Sakai T, Sato S, Kikuchi A, Tei Y, Goda T, Miyahara Y. Preparationof environment-sensitive tetra-gel by using stimuli-sensitive macromer. 3rd International Conference on
Biomaterials Science in Tokyo 2016.11.28 Tokyo, Japan
34. Miyahara Y. Semiconductor devices for biomedical applications. The 3 rd Joint Symposium between
IBB/TMDU and Chulalongkorn University on Biomedical Materials and Engineering 2016.12.15
Bangkok, Thai

― 446 ―

Life
LifeScience
Science andand Technology
Technology

Material-Based Medical Engineering

Prof. Akio KISHIDA
Assoc. Prof. Tsuyoshi KIMURA
Assist. Prof. Yoshihide HASHIMOTO
Research Associate Naoko NAKAMURA
Division of Acellular Tissue and Regenerative Medical Material
Assoc. Prof. Seiichi FUNAMOTO
Lecturer Akitatsu YAMASHITA
Assist. Prof Yongwei ZHANG
Secretary Naomi HIWATARI
Doctor Course Student
Masaki WATANABE(1)

OutlineIn our laboratory, we deal with many research topics from the fundamental study of biomaterials in terms ofmaterial engineering to the application study of the medical devices.
The key words of our policy are "contribution to medical care" and "exploration of basic scientiﬁc principles".

(2)

Research

In order to develop technologies that contribute to the medical and dental care, there is a need for a system tobuild up the design concepts at the molecular level and to realize it. Based on polymer chemistry, organic chemistry, and physical chemistry, we proceed a research aimed at speciﬁc clinical applications using cell engineering,
genetic engineering techniques. Target area are new medical material development, regenerative medicine, genetherapy, and the treatment engineering.
(1) Regenerative medicine using decellularized biological tissue
In order to remove the xenogeneic cells, the living tissue is decellularized using a new processing method, highhydrostatic pressure (HHP) method has been developed. Using this process, complete removal of infectious
bacteria, viruses, and cells was accomplished.
(2) Molecular aggregates formed by the high-hydrostatic pressure process.Hydrogen bond assembles molecularassembly under high pressure. Using the HHP processing of more than 6,000 atmospheres, we prepare thenucleic acid assembly and apply them as gene delivery system.
(3) Extracellular matrix remodeling
We are conducting research on tissue remodeling using artiﬁcially reconstructed extracellular matrix structure.
Speciﬁcally, we are researching the application of as artiﬁcial skin and artiﬁcial cornea of precision design artiﬁcial extracellular matrix structure.

― 447 ―

Life Science
Science andand Technology
Technology
Life(4) Immune control system: technology of speciﬁc cell capture and release
In cancer immunotherapy, by removing regulatory T cells (Treg) that negatively regulates immune reactions,
to be able to enhance the anti-tumor immune responses have been revealed. We are developing technologies tocapture and recover Treg using interfacial science.

(3)

Education

In the Graduate School of Medical and Dental Sciences, we provide the lectures entitled biological functional
materials science , applied biomaterials , and medical, dental and pharmaceutical industrial engineering .
(4)

Publications[Original Articles]
1. Y. Hashimoto, S. Mukai, S. Sawada, Y. Sasaki, K. Akiyoshi. Advanced artiﬁcial extracellular matrices
using amphiphilic nanogel-crosslinked thin ﬁlms to anchor adhesion proteins and cytokines ACS BiomaterSci Eng. 2016.02; 2(3); 375-384
2. Y. Suwa, K. Nam, K. Ozeki, T. Kimura, A. Kishida, T. Masuzawa. Thermal denaturation behavior ofcollagen ﬁbrils in wet and dry environment J Biomed Mater Res Part B: Appl Biomater. 2016.04; 104(3);538-545
3. Y. Hashimoto, S. Hattori, S. Sasaki, T. Honda, T. Kimura, S. Funamoto, H. Kobayashi, A. Kishida.
Ultrastructural analysis of the decellularized cornea after interlamellar keratoplasty and microkeratomeassisted anterior lamellar keratoplasty in a rabbit model Sci Rep. 2016.06; 6; 27734
4. H. Suzuki, Y. Ito, M. Shinohara, S. Yamashita, S. Ichinose, A. Kishida, T. Oyaizu, T. Kayama, R.
Nakamichi, N. Koda, K. Yagishita, MK. Lotz, A. Okawa, H. Asahara. Gene targeting of the transcription
factor mohawk in rats causes heterotopic ossiﬁcation of achilles tendon via failed tenogenesis Proc Natl
Acad Sci USA. 2016.07; 113(28); 7840-7845
5. K. Akazawa, K. Iwasaki, M. Nagata, N. Yokoyama, H. Ayame, K. Yamaki, Y. Tanaka, I. Honda, C.
Morioka, T. Kimura, M. Komaki, A. Kishida, Y. Izumi, I. Morita. Double-layered cell transfer technology
for bone regeneration Sci Rep. 2016.09; 6; 33286
6. T. Kimura, N. Nakamura, N. Sasaki, Y. Hashimoto, S. Sakaguchi, S. Kimura, A. Kishida. Capture andrelease of target cells using a surface that immobilizes an antibody via desthiobiotin–avidin interaction
Sens Mater. 2016.12; 28(12); 1255-1263[Misc]
1. Y. Sawa，K. Matsuda，E. Tatsumi，G. Matsumiya，T. Tsukita，T.Abe，K. Fukunaga，A. Kishida，K.
Kokubo，T. Masuzawa，A. Myoui，M. Nishimura，T. Nishimura，T. Nishinaka, E. Okamoto, S. Tokunaga,
T. Tomo, Y. Yagi, T. Yamaoka. Journal of Artiﬁcial Organs 2015:the year in review Journal of Artiﬁcial
Organs. 2016.03; 19(1); 1-7
2. N. Nakamura, T. Kimura, A. Kishida. Overview of the development, applications, and future perspectivesof decellularized tissues and organs ACS Biomater Sci Eng. 2016.11; 1-9[Conference Activities & Talks]
1. K. S. Furukawa, E. G. Santoso, K. Yoshida, Y. Hirota, O. Yoshino, A. Kishida, T. Hiraoka, Y. Osuga, S.
Saito, T. Ushida. Uterine tissue engineering using a decellularized scaﬀold by hydrostatic pressure. 10th
World Biomaterials Congress 2016.05.17 Montreal,Canada
2. T. Kimura, H. Morita, P. Wu, N. Nakamura, T. Fujisato, A. Kishida. Electrospun ﬁbers covering of
small-diameter decellularized vascular graft for compliance matching. 10th World Biomaterials Congress2016.05.17 Montreal,Canada― 448 ―

Life
LifeScience
Science andand Technology
Technology

3. Ａ. Kishida, Y. Zhang, K. Nam, T. Kimura. Preparation of gradient-type decellularized tissue/polymer
complex for soft tissue-polymer interlinking device. CIMTEC2016 2016.06.05 Perugia, Italy
4. A. Kishida. Biological tissues as new biomaterials. Ireland-Japan Biomaterials and Tissue Engineering
Meeting 2016.06.22 Galway,Ireland
5. A. Kishida. Reconstruction of periodontal tissue using decellularized biological tissue. 2nd Bone andBiomaterials Workshop 2016.08.07 Inari, Finland
6. T. Kimura, P. Wu, N. Nakamura, Y. Hashimoto, A. Yamashita, S. Funamoto, T. Fujisato, A. Kishida. Development of small-diameter decellularized vascular graft combined with electrospun ﬁbers. 2016 Annual
Conference of the Asia-Paciﬁc Society for Artiﬁcial Organs 2016.08.27 Tianjin,China
7. N. Nakamura, T. Kimura, Y. Hashimoto, T. Fujisato, T. Tsuji, A. Kishida. Preparation of decellularized
periodontal ligament for periodontal reconstruction. 2016 Annual Conference of the Asia-Paciﬁc Society
for Artiﬁcial Organs 2016.08.27 Tianjin,China
8. T. Kimura, P. Wu, H. Morita, N. Nakamura, Y. Hashimoto, A. Yamashita, S. Funamoto, T. Fujisato,
A. Kishida. Mechanical and biological properties of small-diameter decellularized vascular graft coveredby electrospun ﬁbers. 9th International Conference on Fiber and Polymer Biotechnology 2016.09.07
osaka,Japan
9. A. Yamashita, Y. Hashimoto, Y. Zhang, S. Funamoto, A. Kishida. Animal model for evaluation ofanti-adhesive materials after open heart surgery. 9th International Conference on Fiber and Polymer
Biotechnology 2016.09.07 Osaka,Japan
10. T. Kimura, Y. Hashimoto, N. Nakamura, Y. Igari, T. Kubota, M. Kondo, S. Higashino, A. Kishida.
Development of decellularaized tissues for controlling stem cell functions. The International Symposium
on Visualization in Joining & Welding Science through Advanced Measurements and Simulation 2016.10.17
Osaka,Japan
11. T. Kimura, N. Nakamura, Y. Hashimoto, S. Sakaguchi, S. Kimura, A. Kishida. Capture and release oflymphocyte on an antibody-immobilized surface. ICBS2016 2016.11.28 Tokyo,Japan
12. N. Nakamura, T. Kimura, Y. Hashimoto, T. Fujisato, T. Tsuji, A. Kishida . Periodontal tissue reconstruction using decellularized PDL matrix and 3D-printed tooth. 2016 TERMIS-Americas Conference &Exhibition 2016.12.11 San Diego,USA
13. T. Kimura, N. Nakamura, Y. Hashimoto, S. Sakaguchi, S. Kimura, A. Kishida . Label-free capture andcollection of target cells using a surface immobilizing antibody. 2016 TERMIS-Americas Conference &Exhibition 2016.12.11 San Diego,USA― 449 ―

Life Science and Technology

Biomolecular Chemistry

Organic and Medicinal Chemistry

Professor Hiroyuki KAGECHIKA
Assistant Professor Syuichi MORI
Assistant Professor Mari YUASAEng Oﬃcial Emiko KAWACHI
Secretary Mayumi SHIKAMA
Graduate Student
Dilihumaer AINIWAER
Yusuke Okazaki
Nozomi Tsuemoto
Sayuri Goryoda
Hidekazu Yokoo
Hiroto Iinuma
Kazuhiro Imaida
Ryohei Iwashita
Tsuyoshi Oikawa
Yuki Noji
Mititake Hirano
Takahiro Miura
Daiki Kato
Chihiro Komatsu
Rie Tsukamoto(1)

Outline1) Medicinal Chemistry of Retinoids
Retinoids regulates various signiﬁcant biological phenomena, such as cell diﬀerentiation, proliferation, morphogenesis, metabolism and homeostasis. We have developed novel synthetic retinoid, Am80 (tamibarotene) as
drug for acute promyelocytic leukemia. Novel synthetic retinoids have been developed foe clinical use in theﬁeld of autoimmune diseases, neurodegenerative diseases, metabolic syndromes.
2) Medicinal Chemistry of Nuclear Receptors
Small hydrophobic molecules such as steroid hormones and activated vitamins A/D control various biologicalphenomena, including growth, development, metabolism, and homeostasis, by binding to and activating speciﬁc nuclear receptors. Nuclear receptors have become one of the most signiﬁcant molecular targets for drug
discovery in the ﬁelds of cancer, metabolic syndrome, autoimmune diseases, and so on. In this project, novel
ligands of various nuclear receptors have been developed.
3) Development of Novel Functional Fluorescent Molecules for Elucidation of Intracellular Signal Transduction
Pathways
Functional ﬂuorescent molecules useful in many ﬁelds of scientiﬁc research, including analytical chemistry orcell biology have been developed.
4) Aromatic Architecture Based on the Steric Properties of N-Methylated Amides
The amide bond structure of amide derivatives often plays a key role in functions such as molecular recognition
events or biological activities. In contrast to the extended trans structures of most secondary amides, the corresponding N-methylated compounds exist in cis form in the crystals and predominantly in cis form in various
solvents. The cis conformational preference is useful as a building block to construct aromatic molecules with― 450 ―

Life Science and Technology

Biomolecular Chemistry
unique crystal or solution structures.

(2)

Lectures & Courses

Organic and Medicinal Chemistry covers several aspects of organic chemistry, medicinal chemistry and chemical
biology. Through this course, students are expected to understand the fundamental knowledge, recent topics,
and experimental techniques related to these ﬁelds.

(3)

Publications[Original Articles]
1. Tsukada, H.; Kawabe, H.; Ohtaka, A.; Saito, Y.: Okamoto, Y.: Tori, M.; Kagechika, H.; Hirota, H.;
Gong, X.; Kuroda, C.; Ohsaki, A.. Two new diterpenoids from Salvia przewarskii Natural ProductCommunications. 2016.01; 11(2); 159-161
2. Persaud, S. D.; Park, S. W.; Ishigami-Yuasa, M.; Koyano-Nakagawa, N.;, Kagechika, H.; Wei, L.-N.. Alltrans-retinoic acid analogs promote cancer cell apoptosis through non-genomic Crabp1 mediating ERK1/2
phosphorylation Science Reports. 2016.03; 6; 22396
3. Shawna D Persaud, Sung Wook Park, Mari Ishigami-Yuasa, Naoko Koyano-Nakagawa, Hiroyuki Kagechika,
Li-Na Wei. All trans-retinoic acid analogs promote cancer cell apoptosis through non-genomic Crabp1
mediating ERK1/2 phosphorylation. Sci Rep. 2016.03; 6; 22396
4. Takemoto, Y.; Ito, A.; Niwa, H.; Okamura, M.; Fujiwara, T.; Hirano, T.; Handa, N.; Umehara, T.; Sonoda,
T.; Ogawa, K.; Tariq, M.; Nishino, N.; Dan, S.; Kagechika, H.; Yamori, T.; Yokoyama, S.; Yoshida, M..
Identiﬁcation of Cyproheptadine as an Inhibitor of SET Domain Containing Lysine Methyltransferase 7/9(Set7/9) That Regulates Estrogen-Dependent Transcription Journol of Medicinal Chemistry. 2016.04;
59(8); 3650-3660
5. Yasushi Takemoto, Akihiro Ito, Hideaki Niwa, Mutsumi Okamura, Takashi Fujiwara, Tomoya Hirano,
Noriko Handa, Takashi Umehara, Takeshi Sonoda, Kenji Ogawa, Mohammad Tariq, Norikazu Nishino,
Shingo Dan, Hiroyuki Kagechika, Takao Yamori, Shigeyuki Yokoyama, Minoru Yoshida. Identiﬁcation of
Cyproheptadine as an Inhibitor of SET Domain Containing Lysine Methyltransferase 7/9 (Set7/9) That
Regulates Estrogen-Dependent Transcription. J. Med. Chem.. 2016.04; 59(8); 3650-3660
6. Shunta Nagashima, Junichi Maruyama, Shodai Kawano, Hiroaki Iwasa, Kentaro Nakagawa, Mari IshigamiYuasa, Hiroyuki Kagechika, Hiroshi Nishina, Yutaka Hata. Validation of chemical compound library
screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci.. 2016.06; 107(6); 791-802
7. Hirano, T.; Noji, Y.; Shiraishi, T.; Ishigami-Yuasa, M.; Kagechika, H.. Development of an OFFON-OFF ﬂuorescent pH sensor suitable for the study of intracellular pH Tetrahedron . 2016.06; 72;4925-4930
8. Nagashima, S.; Maruyama, J.; Kawano, S.; Iwasa, H.; Nakagawa, K.; Ishigami-Yuasa, M.; Kagechika, H.;
Nishina, H.; Hata, Y.. Validation of the chemical compound library screening for TAZ inhibitors by useof green ﬂuorescence protein-fused TAZ Cancer Science. 2016.06; 107(6); 791-802
9. Fujiwara, T.; Ohira, K.; Urushibara, K.; Ito, A.; Yoshida, M.; Kanai, M.; Tanatani, A.; Kagechika, H.;
Hirano, T.. Steric structure–activity relationship of cyproheptadine derivatives as inhibitors of histone
methyltransferase Set7/9 Bioorganic & Medicinal Chemistry. 2016.07; 24; 4318-4323
10. Yokoo, H.; Ohsaki, A.; Kagechika, H.; Hirano T.. Structural development of canthin-5,6-dione moiety as
a ﬂuorescent dye and its application to novel ﬂuorescent sensors Tetrahedron . 2016.08; 72; 5872-5879
11. Tomoya Hirano, Yuki Noji, Takuya Shiraishi, Mari Ishigami-Yuasa, Hiroyuki Kagechika. Development ofan OFF-ON-OFF ﬂuorescent pH sensor suitable for the study of intracellular pH Tetrahedron. 2016.08;
72; 4925-4930― 451 ―

Life Science and Technology

Biomolecular Chemistry

12. Kinoshita, M.; Negishi, M.; Sakai, H.; Hirano, T.; Mori, S.; Fujii, S.; Kagechika, H.; Tanatani, A.. Development of 6-Arylcoumarins as Nonsteroidal Progesterone Antagonists. Structure-activity Relationships
and Fluorescence Properties Bioorganic & Medicinal Chemistry. 2016.09; 24(21); 5602-5610
13. Yamada, A.; Kazui, Y.; Yoshioka, H.; Tanatani, A.; Mori, S.; Kagechika, H.; Fujii, S. . Development of
N-(4-Phenoxyphenyl)benzenesulfonamide Derivatives as Novel Nonsteroidal Progesterone Receptor Antagonists ACS Medicinal Chemistry Letters. 2016.09; 7(12); 1028-1033
14. Takashi Fujiwara, Kasumi Ohira, Ko Urushibara, Akihiro Ito, Minoru Yoshida, Misae Kanai, Aya Tanatani,
Hiroyuki Kagechika, Tomoya Hirano. Steric structure-activity relationship of cyproheptadine derivatives
as inhibitors of histone methyltransferase Set7/9. Bioorg. Med. Chem.. 2016.09; 24(18); 4318-4323
15. Hidetomo Yokoo, Ayumi Ohsaki, Hiroyuki Kagechika, Tomoya Hirano. Structural development of canthin5,6-dione moiety as a ﬂuorescent dye and its application to novel ﬂuorescent sensors Tetrahedron. 2016.09;
72; 5872-5879
16. Okamoto-Uchida, Y.; Yu, R.; Miyamura, N.; Arima, N.; Ishigami-Yuasa, M.; Kagechika, H.; Yoshida,S.; Hosoya, T.; Nawa, M.; Kasama, T.; Asaoka, Y.; Alois, R. W.; Elling, U.; Penninger, J. M.; Nishina,
S.; Azuma, N.; Nishina, H.. The mevalonate pathway regulates primitive streak formation via protein
farnesylation Science Reports. 2016.10; 6; 37697
17. Okamoto-Uchida Y, Yu R, Miyamura N, Arima N, Ishigami-Yuasa M, Kagechika H, Yoshida S, Hosoya
T, Nawa M, Kasama T, Asaoka Y, Alois RW, Elling U, Penninger JM, Nishina S, Azuma N, Nishina H.
The mevalonate pathway regulates primitive streak formation via protein farnesylation Sci Rep. 2016.11;
6; 37697
18. Marie Kinoshita, Mai Negishi, Haruka Sakai, Tomoya Hirano, Shuichi Mori, Shinya Fujii, Hiroyuki
Kagechika, Aya Tanatani. Development of 6-arylcoumarins as nonsteroidal progesterone antagonists.
Structure-activity relationships and ﬂuorescence properties. Bioorg. Med. Chem.. 2016.11; 24(21);5602-5610[Books etc]
1. Research Coaching. Yodosha, 2016.12 (ISBN : 978-4-7581-0159-2)[Conference Activities & Talks]
1. Shuichi Mori, Yusuke Okazaki, Takashi Fujiwara, Asuka Takaguchi,Tomoya Hirano, Hiroyuki Kagechika.
Development of novel assay methods for detection of N-monomethylated lysine using SNAr reaction.
International Symposium for International Symposium for RIKEN Epigenetics Program 2016 2016.02
Saitama
2. Tomoya Hirano, Michitake Hirano, Takashi Fujiwara, Kasumi Ohira, Akihiro Ito, Minoru Yoshida, Hiroyuki Kagechika. Structure-activity relationships of cyproheptadine derivatives as inhibitors of histone
methyltransferase, Set7/9. International Symposium for International Symposium for RIKEN Epigenetics
Program 2016 2016.02 Saitama
3. Hirano, T., Hirano, M., Fujiwara, T., Ohira, K., Ito, A., Yoshida, M., Kagechika, H. . Structure-activity
relationships of cyroheptadine as inhibitors of histone methyltransferase, Set7/9. International symposiumfor RIKEN epigenetics program 2016.02 Tokyo
4. Mori, S., Okazaki, Y., Fujiwara, T., Takaguchi, A., Hirano, T., Kagechika, H.. Development of novel
assay methods for detection of N-monomethylated lysine using SNAr reaction. International symposiumfor RIKEN epigenetics program, 2016.02 Tokyo
5. Tomoya Hirano, Takuya Shiraishi, Yuki Noji, Daiki Kato, Hiroyuki Kagechika. Development and biological application of ﬂuorescent sensors for microenvironmental change. The 96th CSJ Annual Meeting2016.03.25 Kyoto
6. Takuya Shiraishi, Tomoya Hirano, Toshiki Saito, Hiroyuki Kagechika. Development of ﬂuorescent sensors
for zinc ion activated by enzymatic reaction. The 96th CSJ Annual Meeting 2016.03.26 Kyoto― 452 ―

Life Science and Technology

Biomolecular Chemistry

7. Ishigami-Yuasa M, Ekimoto H, Kagechika H.. Synergistic inhibition of several human cancer cell proliferations by a synthetic retinoid tamibarotene (Am80) in combination with the epigenetic modulators.. The
3RD International conference on Retinoids 2016.06.19 Florida, USA
8. Kagehcika, H.. Development of novel retinoids with unique hydrophobic pharmacophore. FASEB meetingon retinoid 2016.06.20 Florida, USA
9. Hirano, T., Shiraishi, T., Noji, Y., Kato, D., Kagechika, H.. Development of ﬂuorescent sensors for
microenvironmental change based on ﬂuorescent compounds library. 252nd ACS National Meeting 2016.08
Philadelphia, USA
10. Yokoo, H., Hirano, T., Ohsaki, A., Kagechika, H.. Development of Novel Fluorescent Sensors Based on
Fluorescent Natural Compounds. 252nd ACS National Meeting 2016.08 Philadelphia, USA
11. Tanatani, A., Tojo, Y., Urushibara, K., Yamamoto, S., Mori, H., Masu, H., Kudo, M., Hirano, T.,
Kagechika, H.. N-Alkylated Pyrroleamide Oligomers with Folded Cis-Amide Conformation. Symposium
on Foldamers 2016 2016.09.26 Bordeaux-Pessac, France
12. Mori, S., Oshida, Y., Watanabe, Y., Yuasa, M., Kagechika, H.. Development of novel store-operated
calcium entry inhibitors. Int. Chem. Biol. Symp 2016.10 Madison, USA
13. Okazaki, Y., Fujiwara, T., Takaguchi, A., Hirano, T., Mori, S., Kagechika, H.. Development of a facile
method to detect the activity of histone methyltransferase based on SNAr reaction. Int. Chem. Biol.
Symp 2016.10 Madison, USA
14. Rawangkan, A., Oya, Y., Iida, K., Kagechika, H., Shudo, K., Fujiki, H., Suganuma, M. . Innovative
strategy of cancer treatment with the combination of Am80, a potent synthetic retinoid, and green tea
catechin via down-regulation ofhistone deacetylase 4, -5, and -6. Int. Symp. Biomed. Engineer.
2016.11.10 Tokyo
15. Hirano, T., Noji, Y., Kato, D., Shiraishi, T., Kagechika, H.. Development of ﬂuorescent sensors for
microenvironemtal change. Int. Symp. Biomed. Engineer. 2016.11.10 Tokyo
16. Mori, S., Takagaki, R., Fujii, S., Matsumura, M., Tanatani, A., Kagechika, H.. Development of mcarborane-based progesterone receptor ligands and their optical resolution by lipase reaction. Int. Symp.
Biomed. Engineer. 2016.11.10 Tokyo
17. Ishigam-Yuasa, M., Ekimoto, H., Kagechika, H.. Synergistic inhibition of several human cancer cellproliferations by a synthetic retinoid tamibarotene (Am80) in combination with the epigenetic modulators.
Int. Symp. Biomed. Engineer. 2016.11.10 Tokyo
18. Kagechika, H.. Development of Functional Molecules with Unique Scaﬀolds. The 3rd Joint Symp.
IBB/TMDU & Chulalongkorn Univ. Biomed. Mater. Engineer. 2016.12.15 Bangkok
19. Ishigami-Yuasa, M., Ekimoto, H., Kagechika, H.. Synergistic inhibition of several human cancer cellproliferations by a synthetic retinoid tamibarotene (Am80) in combination with the epigenetic modulators.
FASEB meeting on retinoid Florida, USA
20. Tsuemoto, N., Mori, S., Kawachi, E., Kagechika, H.. Design and synthesis of novel RAR ligands containing
pentaﬂuorosulfanyl group. FASEB meeting on retinoid Florida, USA[Patents]
1. Lck binding protein, Patent Number：United States Patent 5891673
2. IKK3 kinase, Patent Number：United States Patent 6576439
3. A protein kinase (IKK4) involved in the phosphorrylation of IKB, Patent Number：WO/2001/044444[Works]
1. Expression Vectors http://dna.brc.riken.jp/search/dep3357.html, Other, RIKEN[Awards & Honors]
1. The Vitamin Society of Japan Award, Vitamin Society of Japan, 2016.06― 453 ―

Life Science and Technology

Life Science and Technology

Chemical Bioscience

Professor
Associate Professor
Assistant Professor
Technical AssistantGraduate Students

Collaborators(1)

Takamitsu HOSOYA
Suguru YOSHIDA
Yoshitake NISHIYAMA
Yoshihiro MISAWA, Yuki HAZAMA,
Tomoko KURIBARA
Takamoto MORITA, Kazuya KANEMOTO,
Keisuke UCHIDA, Tomohiro MEGURO,
Kei AKIYAMA, Kazuhiro OYA,
Akira NAGAI, Hana NAKAJIMA,
Yu NAKAMURA, Naoaki MAKIO,
Kotaro MUTSUURA, Shonan CHIN,
Youngchan KIM, Tsubasa MATSUZAWA,
Yuya TAMURA
Shuhei KAMADA, Yoshihiri MIYATA
Keita SHIMIZU, Norikazu TERASHIMA

OutlineDevelopment of new organic synthetic methods, new chemical methodologies, and new chemical tools, those areuseful for biological and drug discovery researches.
(2)

Research

1) Development of novel generation methods for benzyne species and their synthetic applications.
2) Development of new bioconjugation methods using strained alkynes.
3) Target identiﬁcation of drugs or drug candidates by photoaﬃnity labeling based on diazido probe method..
4) Development of new molecular ligation methods based on new azido chemistry.
5) Design and synthesis of eﬃcient substrates for bioluminescence reactions and ﬂuorescent probes for bioimaging
and diagnosis of diseases.
6) Design and synthesis of new PET (positron emission tomography) probe candidates for in vivo imaging topromote drug discovery.
(3)

Publications[Original Articles]
1. Isao Kii, Yuto Sumida, Toshiyasu Goto, Rie Sonamoto, Yukiko Okuno, Suguru Yoshida, Tomoe KatoSumida, Yuka Koike, Minako Abe, Yosuke Nonaka, Teikichi Ikura, Nobutoshi Ito, Hiroshi Shibuya,
Takamitsu Hosoya, Masatoshi Hagiwara. Selective inhibition of the kinase DYRK1A by targeting its
folding process. Nat Commun. 2016; 7; 11391
2. Tomabechi Y, Hosoya T, Ehara H, Sekine S, Shirouzu M, Inouye S. Crystal structure of nanoKAZ:
The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction withcoelenterazine. Biochem Biophys Res Commun. 2016.01; 470(1); 88-93― 454 ―

Life Science and Technology

Life Science and Technology

3. Uetake Y, Niwa T, Hosoya T. Rhodium-Catalyzed ipso-Borylation of Alkylthioarenes via C-S Bond Cleavage Org Lett. 2016.06; 18(11); 2758-2761
4. Yoshida S, Morita T, Hosoya T. Synthesis of Diverse Benzotriazoles from Aryne Precursors Bearing an
Azido Group via Inter- and Intramolecular Cycloadditions Chem Lett. 2016.07; 45(7); 726-728
5. Yoshida S, Shimomori K, Kim Y, Hosoya T. Single C-F Bond Cleavage of Triﬂuoromethylarenes with an
ortho-Silyl Group Angew Chem Int Ed Engl. 2016.08; 55(35); 10406-10409
6. Controlled Generation of 3-Triﬂyloxyarynes Synthesis. 2016.08; 48(23); 4099-4109
7. Tanaka T, Okuyama-Dobashi K, Murakami S, Chen W, Okamoto T, Ueda K, Hosoya T, Matsuura Y,
Ryo A, Tanaka Y, Hagiwara M, Moriishi K. Inhibitory eﬀect of CDK9 inhibitor FIT-039 on hepatitis B
virus propagation Antiviral Res. 2016.09; 133; 156-164
8. Yoshida S, Yano T, Nishiyama Y, Misawa Y, Kondo M, Matsushita T, Igawa K, Tomooka K, Hosoya T.
Thiazolobenzyne: a versatile intermediate for multisubstituted benzothiazoles Chem Commun. 2016.09;
52(75); 11199-11202
9. Doi H, Sato K, Shindou H, Sumi K, Koyama H, Hosoya T, Watanabe Y, Ishii S, Tsukada H, Nakanishi
K, Suzuki M. Blood-brain barrier permeability of ginkgolide: Comparison of the behavior of PET probes
7 α-[ 18 F] ﬂuoro- and 10-O-p-[ 11 C] methylbenzyl ginkgolide B in monkey and rat brains Bioorg Med
Chem. 2016.11; 24(21); 5148-5157
10. Sumida Y, Sumida T, Hashizume D, Hosoya T. Preparation of Aryne-Nickel Complexes from orthoBorylaryl Triﬂates Org Lett. 2016.11; 18(21); 5600-5603
11. Okamoto-Uchida Y, Yu R, Miyamura N, Arima N, Ishigami-Yuasa M, Kagechika H, Yoshida S, Hosoya
T, Nawa M, Kasama T, Asaoka Y, Alois RW, Elling U, Penninger JM, Nishina S, Azuma N, Nishina H.
The mevalonate pathway regulates primitive streak formation via protein farnesylation Sci Rep. 2016.11;
6; 37697
12. Ochiai H, Niwa T, Hosoya T. Stereoinversion of Stereocongested Carbocyclic Alcohols via Triﬂylation and
Subsequent Treatment with Aqueous N,N-Dimethylformamide Org Lett. 2016.12; 18(23); 5982-5985
13. Yoshida S, Nakamura Y, Uchida K, Hazama Y, Hosoya T. Aryne Relay Chemistry en Route to Aminoarenes:
Synthesis of 3-Aminoaryne Precursors via Regioselective Silylamination of 3-(Triﬂyloxy)arynes. Org Lett.
2016.12; 18(23); 6212-6215[Conference Activities & Talks]
1. Uchida K, Yoshida S, Igawa K, Tomooka K, Hosoya T. An eﬃcient generation method and remarkable
reactivities of 3-triﬂyloxybenzyne. The 12th International Symposium on Organic Reactions (ISOR-12)
2016.04.22 Kyoto, Japan
2. Nakamura Y, Uchida K, Hazama Y, Yoshida S, Hosoya T. Facile Synthesis of 3-Aminoaryne Precursors via
Aminosilylation of 3-Triﬂyloxyarynes. The 12th International Symposium on Organic Reactions (ISOR12) 2016.04.23 Kyoto, Japan
3. Yoshida S, Uchida K, Nakamura Y, Nonaka T, Morita T, Nagai A, Igawa K, Tomooka K, Hosoya T.
Trimethylsilylmethyl Grignard Reagent: a Mild Activator Triggering Eﬃcient Generation of Arynes
from Various ortho-Iodoaryl Triﬂates. 11th International Symposium on Carbanion Chemistry (ISCC11) 2016.07.20 Rouen, France
4. Hosoya T. Azido-type selective reactions for target identiﬁcation and probe synthesis. 3rd Symposium of
SPU Innovative Project for Pharmaceutical Analyses of Covalent Modiﬁcation in Biomolecules "ChemistryNatural Product Chemistry, Structural Chemistry, Synthetic Chemistry and Chemical Biology" 2016.09.01
Tokyo, Japan― 455 ―

Life Science and Technology

Advanced Therapeutic Sciences

Biomechanics

Kenji Kawashima
Takahiro Kanno(1)

OutlineOur Lab. mainly working on the development of medical devises and systems based on control engineering,robotics and ﬂuid dynamics.
Key word is system integration such as hardware and software, electrical and pneumatics, human and machine.
(2)

Research

1)Forceps manipulator for minimally invasive surgery2)Power assist devices using pneumatic actuators
3)Medical devices using soft actuators4)Surgical robot system
5)Tele-operation of robots using biological information(3)

Education

Learn about mechanical design and control engineering for medical devices based on biomechanics. Master abasic skill to develop the devices from the researchers and engineers working on the medical devices and systems.
Learn the basic control method of a surgical robot using a personal computer.
(4)

Lectures & Courses

The object is to provide the ability to design and develop medical devices based on biomechanics, which studies
the structure and function of biological systems, with mechanical dynamics, robotics and control engineering.
(5)

Publications[Original Articles]
1. Hongbing Li, Kenji Kawashima. Bilateral teleoperation with delayed force feedback using time domain
passivity controller Robotics and Computer-Integrated Manufacturing. 2016.02; 37; 188-196― 456 ―

Life Science and Technology

Advanced Therapeutic Sciences

2. Mohammadreza Kamali, Seyed Ali Jazayeri, Farid Najaﬁ, Kenji Kawashima, Toshiharu Kagawa. Integrated Nozzle-Flapper Valve with Piezoelectric Actuator and Isothermal Chamber; A Feedback Linearization Multi-Control Device Journal of Mechanical Science and Technology. 2016.05; 20(5); 2293-2301
3. Mohammadreza Kamali, Seyed Ali Jazayeri, Farid Najaﬁ, Kenji Kawashima, Toshiharu Kagawa. Studyon Performance and Control of Piezo-Acuated Nozzle-Flapper Valve with Isothermal Chamber Journal of
Mechanical Engineering. 2016.05; 62(5); 318-328
4. Haruka Sakurai, Takahiro Kanno, Kenji Kawashima . Thin-diameter Chopsticks Robot for Laparoscopic
Surgery IEEE International Conference on Robotics and Automation. 2016.05; 4122-4127
5. Kengo Watanabe, Takahiro Kanno, Kazuhisa Ito, Kenji Kawashima. Human-Integrated Automation of
suturing task with One-Master Two-Slave System for Laparoscopic Surgery IEEE International Conference
on Advanced Intelligent Mechatronics. 2016.07; 1180-1185
6. Kyouhei Takikawa, Ryoken Miyazaki, Takahiro Kanno, Gen Endo, Kenji Kawashima. Pneumaticallydriven multi-DOF surgical forceps manipulator with a bending joint using continuums Journal of Robotics
and Mechatronics. 2016.08; 28(4); 559-567
7. Sho Yoshida, Takahiro Kanno, Kenji Kawashima,. A Novel RCM Mechanism Using Pneumatically Driven
Flexible Joint for Laparoscopic Forceps Holder IEEE International Symposium on Robot and Human
Interactive Communication (RO-MAN 2016). 2016.08;
8. Ryoken Miyazaki, Takahiro Kanno, Kenji Kawashima,. Pneumatically-Driven Hand-held Forceps with
Wrist Joint Operated by Built-in Master Controller IEEE ICMA 2016. 2016.09; 442-447
9. Masahiko Minamoto, Kenji Kawashima, Takahiro Kann. Eﬀect of Force Feedback on a Bulldozer-Type
Robot IEEE ICMA 2016. 2016.09; 2203-2208
10. Hongbing Li, Kenji Kawashima. Achieving Stable Tracking in Wave-Variable-Based Teleoperation. (ACCEPT) IEEE/ASME Transactions on Mechatronics (TMECH).

― 457 ―

Life Science and Technology

Life Science and Technology

Molecular Cell Biology

Professor Hiroshi Shibuya
Associate Professor Toshiyasu Goto
Assistant Professor Atsushi Sato(1)

Lectures & Courses

Various signaling molecules inducing the cell-growth and diﬀerentiation regulate morphogenesis and organogenesis of the vertebrate. The failure of these signal molecules has also been caused with induction of the diseases.
Therefore, the elucidation of signal transduction network regulating generation and diﬀerentiation is important
upon clarifying the mechanism of morphogenesis, organogenesis and diseases. Our research aim is to clarify thesignal transduction network regulating the mechanisms of morphogenesis and organogenesis in developmental
process. We serve these research and following education to provide graduate students who will become seniorscientists in life sciences.
(2)

Publications[Original Articles]
1. Isao Kii, Yuto Sumida, Toshiyasu Goto, Rie Sonamoto, Yukiko Okuno, Suguru Yoshida, Tomoe KatoSumida, Yuka Koike, Minako Abe, Yosuke Nonaka, Teikichi Ikura, Nobutoshi Ito, Hiroshi Shibuya,
Takamitsu Hosoya, Masatoshi Hagiwara. Selective inhibition of the kinase DYRK1A by targeting its
folding process. Nat Commun. 2016; 7; 11391
2. Takashi Fukuzono, Strahil Iv Pastuhov, Okinobu Fukushima, Chun Li, Ayuna Hattori, Shun-Ichiro Iemura,
Tohru Natsume, Hiroshi Shibuya, Hiroshi Hanafusa, Kunihiro Matsumoto, Naoki Hisamoto. Chaperone
complex BAG2-HSC70 regulates localization of Caenorhabditis elegans leucine-rich repeat kinase LRK-1
to the Golgi. Genes Cells. 2016.04; 21(4); 311-324
3. Toshiyasu Goto, Junhei Matsuzawa, Shun-Ichiro Iemura, Tohru Natsume, Hiroshi Shibuya. WDR26 is a
new partner of Axin1 in the canonical Wnt signaling pathway. FEBS Lett.. 2016.05; 590(9); 1291-1303
4. Ogawa Y., Nonaka Y., Goto T., Ohnishi E., Hiramatsu T., Kii I., Yoshida M., Ikura T., Onogi H., Shibuya
H., Hosoya T., Ito N., Hagiwara M.. Development of a novel selective inhibitor of the Downsyndromerelated kinase Dyrk1A. (INPRES) Nat. Commun..

― 458 ―

Life
LifeScience
Science andand Technology
Technology

Developmental and Regenerative Biology

Professor Hiroshi Nishina, Ph.D.
Associate Professor Jun Hirayama, Ph.D.
Assistant Professor Norio Miyamura, Ph.D.
Technical Assistants Yumi Akagawa
Secretary Kazuko Tanaka(1)

OutlineOur goal is to deﬁne the molecular basis for the mechanism of organ formation and regeneration using knockout
mice and mutant ﬁshes. To accomplish this goal, we have focused on deﬁning signaling molecules and pathways
that regulate liver formation and stress responses. Moreover, we are trying to establish a cell therapy for
intractable diseases such as liver failures using self-bone marrow cells. Our study will provide new insights into
understanding the precise molecular mechanisms that underlie organ failures found in human disease and willlead to the development of new rational therapy for the diseases.
(2)

Research

1) Studies on the stress-activated protein kinase (SAPK/JNK) signaling pathway
2) Studies on the Hippo signaling pathway
3) Studies on the cell diﬀerentiation of mouse ES cells
4) Studies on liver formation using a small ﬁsh, Medaka, Oryzias Latipes5) Studies on liver regeneration using mice6) Studies on circadian clock using zebraﬁsh and mice(3)

Publications[Original Articles]
1. Miki Nishio, Keishi Sugimachi, Hiroki Goto, Jia Wang, Takumi Morikawa, Yosuke Miyachi, Yusuke
Takano, Hiroki Hikasa, Tohru Itoh, Satoshi O Suzuki, Hiroki Kurihara, Shinichi Aishima, Andrew Leask,
Takehiko Sasaki, Toru Nakano, Hiroshi Nishina, Yuji Nishikawa, Yoshitaka Sekido, Kazuwa Nakao, Kazuo
Shin-Ya, Koshi Mimori, Akira Suzuki. Dysregulated YAP1/TAZ and TGF-β signaling mediate hepatocarcinogenesis in Mob1a/1b-deﬁcient mice. Proc. Natl. Acad. Sci. U.S.A.. 2016.01; 113(1); E71-E80
2. Yusuke Nasu, Yoichi Asaoka, Misako Namae, Hiroshi Nishina, Hideaki Yoshimura, Takeaki Ozawa. Genetically Encoded Fluorescent Probe for Imaging Apoptosis in Vivo with Spontaneous GFP Complementation. Anal. Chem.. 2016.01; 88(1); 838-844
3. Koichi Fujisawa, Shuji Terai, Taro Takami, Naoki Yamamoto, Takahiro Yamasaki, Toshihiko Matsumoto,
Kazuhito Yamaguchi, Yuji Owada, Hiroshi Nishina, Takafumi Noma, Isao Sakaida. Modulation of anticancer drug sensitivity through the regulation of mitochondrial activity by adenylate kinase 4. J. Exp.
Clin. Cancer Res.. 2016.03; 35; 48
4. Yoichi Asaoka, Yoko Nagai, Misako Namae, Makoto Furutani-Seiki, Hiroshi Nishina. SLC7 family transporters control the establishment of left-right asymmetry during organogenesis in medaka by activating
mTOR signaling. Biochem. Biophys. Res. Commun.. 2016.05; 474(1); 146-153― 459 ―

Life Science
Science andand Technology
Technology
Life

5. Takanori Chiba, Erika Ishihara, Norio Miyamura, Rika Narumi, Mihoko Kajita, Yasuyuki Fujita, Akira
Suzuki, Yoshihiro Ogawa, Hiroshi Nishina. MDCK cells expressing constitutively active Yes-associatedprotein (YAP) undergo apical extrusion depending on neighboring cell status. Sci Rep. 2016.06; 6; 28383
6. Shunta Nagashima, Junichi Maruyama, Shodai Kawano, Hiroaki Iwasa, Kentaro Nakagawa, Mari IshigamiYuasa, Hiroyuki Kagechika, Hiroshi Nishina, Yutaka Hata. Validation of chemical compound library
screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci.. 2016.06; 107(6); 791-802
7. Okamoto-Uchida Y, Yu R, Miyamura N, Arima N, Ishigami-Yuasa M, Kagechika H, Yoshida S, Hosoya
T, Nawa M, Kasama T, Asaoka Y, Alois RW, Elling U, Penninger JM, Nishina S, Azuma N, Nishina H.
The mevalonate pathway regulates primitive streak formation via protein farnesylation Sci Rep. 2016.11;
6; 37697― 460 ―

Life
LifeScience
Science andand Technology
Technology

Immunology

Professor: Takeshi TSUBATA, M.D., Ph.D.
Associate Professor: Takahiro ADACHI, Ph.D.
Assistant Professors: Naoko MATSUBARA Ph.D.
Specially Appointed Assistant Professor: Chizuru AKATSU Ph.D.
Specially Appointed Junior Associate Professor: Ji-Yang WANG Ph.D.
Project Researcher: Miao Tang Ph.D., Toshitaro Takata, Ph.D., Mohanmmad Aslam, Ph.D.
Technicians: Yukie KURUSU, Shigeko NAKANOSecretary: Chikako SAWADA
Graduate Students: Nazim Medzhidov, Yang-Yang Feng
Graduate Students: Amin ALBORZIAN DESHEIKH,
Graduate Students: Sundararaman RENGARAJAN,
Graduate Students: Yang Hongrui, Li Xuexin(1)

Research

The nature of immune responses depends on whether they respond to protein or non-protein antigens because
T lymphocytes recognize only protein antigens. Normal immune system removes pathogens and cancer cells but
does not respond to non-microbial foreign substances or self-antigens. Immune responses to non-microbial foreign substances and self-antigens cause allergy and autoimmune diseases, respectively. How the immune system
distinguishes pathogens from non-microbial antigens and self-antigens is already clariﬁed for protein antigens.
However, little is know about such distinction for non-protein antigens. Immune responses to non-protein antigens play crucial roles in host defense against pathogens such as tuberculosis bacilli and meningococci, andautoimmune diseases such as lupus and immuno-neurological disorders. Thus, immune responses to non-protein
antigens constitute a remaining frontier in immunology research. Followings are our research subjects.
(2)

Education

Lecture course on immunology at the master course aims at giving the students the basic ideas how immune
system recognize and respond to the antigens, and how immune system eﬃciently remove pathogens without
responding to self-antigens and environmental antigens. In the lecture course in bioscience at the doctor course,
lectures on immune responses are given so that the students are introduced with the current topics in theﬁeld of humoral immune responses. Research projects in both master and doctor courses aims at training thestudents to acquire basic research techniques on immunology, molecular biology and biochemistry, and abilities
to conduct cutting-edge research in the ﬁeld of immunology by themselves under supervision.
(3)

Publications[Original Articles]
1. Li, Y., Takahashi, Y., Fujii, S., Suzuki, A., Tsubata, T., Hase, K. and Wang, J-Y.. EAF2 mediates
germinal center B cell apoptosis to suppress excessive immune responses and prevent autoimmunity Nature
Communications. 2016; 7(10836);― 461 ―

Life Science
Science andand Technology
Technology
Life

2. Tsubata, T.. CD22 and CD72 are inhibitory receptors dominantly expressed in B lymphocytes andregulate systemic autoimmune diseases. Z. Rheumatol.. 2016; 75; 86-89
3. Akatsu, C., Shinagawa, K., Numoto, N., Liu, Z., Konuscan, A.U., Aslam, M., Phoon, S., Adachi, T.,
Furukawa, K., Ito, N. and Tsubata. T.. CD72 negatively regulates B lymphocyte responses to the lupusrelated endogenous Toll-like receptor 7 ligand Sm/RNP. J. Exp. Med.. 2016; 213; 2691-2706
4. Adachi T, Kakuta S, Aihara Y, Kamiya T, Watanabe Y, Osakabe N, Hazato N, Miyawaki A, Yoshikawa
S, Usami T, Karasuyama H, Kimoto-Nira H, Hirayama K, Tsuji NM.. Visualization of Probiotic-Mediated
Ca2+ Signaling in Intestinal Epithelial Cells In Vivo. Front Immunol.. 2016; 7(601);[Books etc]
1. Li, Y., Takahashi, Y., Fujii, S., Suzuki, A., Tsubata, T., Hase, K. and Wang, J-Y.. EAF2 mediates
germinal center B cell apoptosis to suppress excessive immune responses and prevent autoimmunity.
Nature Communications, 2016
[Conference Activities & Talks]
1. Kai Jin, Toshihiro Imada, Yusuke Izuta, Shigeru Nakamura, Takahiro Adachi, Kazuo Tsubota. IN VIVO
VISUALIZATION OF Ca2+ DYNAMICS OF MYOEPITHELIAL CELLS IN LACRIMAL GLAND. 8thInternational Conference on the
Tea Film & Ocular Surface Basic Science and Clinical Relevance2016.09.08 FRANCE
2. Chizuru Akatsu,Kenro Shinagawa, Nobutaka Numoto, Zhihong Liu, Mohommad Aslam, Nobutoshi Ito,
Takeshi Tsubata. B cells reactive to Sm/RNP, a lupus-related self-antigen. 12th International Conference
on Protein Phosphatase (ICPP12) 2016.10.28 OSAKA
3. Takeshi Tsubata. B lymphocytes and autoimmunity. Fudan University School of Basic Medical Sciences,
China 2016.11.07 SHANGHAI,CHINA
4. Takeshi Tsubata. Development of a novel glycomimetic with unique immunological activity. . 15th
Surugadai International Symposium "Current Status and Future of Drug Discovery" 2016.11.29 TOKYO
5. TSUJI Noriko M, KAMIYA Tomonori, WATANABE yohei,KAKUTA Shigeru, ADACHI Takahiro. Eﬀects
of fermented food on immune response and gut microbiota of human faecal ﬂora-associated (HFA) mice.
The 45th Annual Meeting of The Japanese Society for Immunology (JSI) 2016.12.05 OKINAWA
6. Medzhidov Nazim, Takata toshitaro, Suzuki Mitsuhiro, Ichinose Shizuko, Tsubata Takeshi. Distinct
ubiquitination level and sorting of B cell receptor. The 45th Annual Meeting of The Japanese Society for
Immunology (JSI) 2016.12.06 OKINAWA
7. ADACHI Takahiro, KARASUYAMA Hajime , YOSHIKAWA Soichiro. Visualization of immune responses
by intravital imaging of Ca2+ signals. The 45th Annual Meeting of The Japanese Society for Immunology(JSI) 2016.12.07 OKINAWA― 462 ―

Life
LifeScience
Science andand Technology
Technology

Epigenetics

Professor Fumitoshi Ishino
Associate Professor Takashi Kohda
Assistant Professor Hirosuke Shiura
Assistant Professor Yuki Kawasaki
Project Lecturere Jiyoung Lee
Adjanct Lecturere Shin Kobayashi(1)

OutlineEpigenetics and Genetics are basics of biology that enables us to elucidate several genomic functions ininheritance, development and evolution of the organisms including our human beings. Genomic imprinting isa mammalian-speciﬁc epigenetic mechanism that gives rise to functional diﬀerences between paternally- andmaternally-derived genomes in development, behavior and growth. Somatic cloned animals give us uniquechances to examine genetically identical but epigenetically diverged animals . Mammalian-speciﬁc LTR
retrotransposon-derived genes are essential for mammalian development, such as placenta and brain functions.
These studies show us how Epigenetics and Genetics are important in mammalian biology. We focus on thesemammalian-speciﬁc genomic functions to elucidate how these genomic functions work and have been evolved
as new genomic functions during evolution. Our ﬁnal goal is to contribute to the human biology as well as
medicine in the 21st century by novel understanding of genomic functions.
(2)

Research

1) Genomic imprinting in human and mammalian development.
2) Placenta function and its evolution in mammals.
3) Somatic cloning: its epigenetic eﬀects and application to regenerative medicine.
4) Assisted reproductive technology: its epigenetic eﬀects and safer application.
5) Role of retrotransposon-derived genes in mammalian speciﬁc genomic functions.

(3)

Education

Graduate School of Medical and Dental Science
Life Science and Technology Track
Bioscience I
Medical and Dental Science and Technology Track
Molecular and Cellular Biology
Develeopmenta and Regenerative Bioscience
Molecular Cell Biology
Introduction to Human Molecular Genetics
Faculty of Medicine
Molecular Genetics― 463 ―

Life Science
Science andand Technology
Technology
Life

(4)

Lectures & Courses

Obtain basic and applicative knowledges and techniques by challenging cutting-edge themes.
(5)

Clinical Services & Other Works

Research Center for Science Systems, Senior Researcher
Japan Society for Biological Sciences, Board member
Japan Society for Epigenetics, Secretary(6)

Publications[Original Articles]
1. Masahito Irie, Akihiko Koga, Tomoko Kaneko-Ishino, Fumitoshi Ishino. An LTR Retrotransposon-Derived
Gene Displays Lineage-Speciﬁc Structural and Putative Species-Speciﬁc Functional Variations in Eutherians. Front Chem. 2016.06; 4; 26
2. Kobayashi S*, Hosoi Y, Shiura H, Yamagata K, Takahashi S, Fujihara Y, Kohda T, Okabe M and Ishino
F.. Live imaging of X chromosome reactivation dynamics in early mouse development can discriminatenaïve from primed pluripotent stem cells. Development. 2016.08; 143(16); 2958-2964
3. Yuki Kawasaki, Yukiko Kuroda, Isao Suetake, Shoji Tajima, Fumitoshi Ishino, Takashi Kohda. A Novelmethod for the simultaneous identiﬁcation of methylcytosine and hydroxymethylcytosine at a single baseresolution. Nucleic Acids Res.. 2016.10; 45(4); e24[Conference Activities & Talks]
1. Masahito Irie, Tomoko Kaneko-Ishino, Fumitoshi Ishino. Cognitive function regulated by an LTR retrotransposonderived gene, Sirh11/Zcchc16. . 1st Korea-Japan International Symposium for Transposable Elements
2016.06.10 Busan, Korea
2. Fumitoshi Ishino. Mammalian-speciﬁc genomic functions: Newly acquired traits generated by genomic
imprinting and LTR retrotransposon-derived genes in mammals. . Annual Meeting of Korean Society forLife Science 2016.08.22 K-Hotel, Gyeongju, Korea
3. Fumitoshi Ishino, Tomoko Kaneko-Ishino. . Mammalian evolution by gene acquisition from Retrotransposons.. The 20th Evolutionary Biology Meeting at Marseilles 2016.09.21 CRDP, Marseilles, France
4. Tomoko Kaneko-Ishino, Masahito Irie, Akihiko Koga, Fumitoshi Ishino.. An LTR retrotransposon derived
SIRH11/ZCCHC16 gene involved in cognitive function displays lineage-speciﬁc structural and putative
species-speciﬁc functional variations in eutherians. . The 20th Evolutionary Biology Meeting at Marseilles
2016.09.22 CRDP, Marseilles, France
5. Futmitoshi Ishino, Tomoko Kaneko-Ishino. Fumitoshi Ishino. Regulation of placental function by mammalianspeciﬁc genes from retrotransposons.. The 2nd IMCR Symposium on Endocrine and Metabolism:International
Frontiers in Homeostatic Regulation Research 2016.11.21 Tojo Hall, Gunma University, Gunma
6. Fumitoshi Ishino, Tomoko Kaneko-Ishino. . Mammalian evolution by gene acquisition from retrotransposons. . Symposium : Unidentiﬁed functions of the genome, The 39th Annual Meeting of MBSJ
2016.12.01 Paciﬁco Yokohama,Yokohama― 464 ―

Life Scienceand Technology
MedicalGenomics

Medical Science Mathematics

Professor: Tatsuhiko Tsunoda, Junior Associate Professor: Daichi Shigemizu, Assistant Professor: Fuyuki Miya
(1)

OutlineMedical application of rapidly progressing omic proﬁling technologies and, in particular, the promotion of personalized/precision/preventive medicine have been keenly desired. Our department overcomes such medical scienceissues by using a combination of mathematics and computational sciences: (1) Integrative analysis of clinical andomic data for exploring etiologies of intractable diseases, (2) Molecular classiﬁcation of and systems approach tounderstanding disease based on omic proﬁling, and (3) Prediction for personalized/precision/preventive medicine- we apply mathematical methods, e.g, machine learning techniques, to optimum therapy prediction for eachpatient when she/he visits to a hospital/medical institute, and we can also apply these methods to disease
prevention based on an individual s health check records.

(2)

Publications[Original Articles]
1. Cristian Pattaro, ..., Madhumathi Rao, Maksim Struchalin, Marcus E Kleber, Margherita Cavalieri, Margot Haun, Marilyn C Cornelis, Marina Ciullo, Mario Pirastu, Mariza de Andrade, Mark A McEvoy, Mark
Woodward, Martin Adam, Massimiliano Cocca, Matthias Nauck, Medea Imboden, Melanie Waldenberger,
Menno Pruijm, Marie Metzger, Michael Stumvoll, Michele K Evans, Michele M Sale, Mika Kähönen,Mladen Boban, Murielle Bochud, , , , , , , , ICBP Consortium; AGEN Consortium; CARDIOGRAM;
CHARGe-Heart Failure Group; ECHOGen Consortium, , , , , , , , Tonu Esko, Toshiko Tanaka, Ulf
Gyllensten, Uwe Völker, Valur Emilsson, Veronique Vitart, Ville Aalto, Vilmundur Gudnason, Vincent
Chouraki, Wei-Min Chen, Wilmar Igl, Winfried März, Wolfgang Koenig, Wolfgang Lieb, Ruth J F Loos,
Yongmei Liu, Harold Snieder, Peter P Pramstaller, Afshin Parsa, Jeﬀrey R O’Connell, Katalin Susztak,
Pavel Hamet, Johanne Tremblay, Ian H de Boer, Carsten A Böger, Wolfram Goessling, Daniel I Chasman,Anna Köttgen, W H Linda Kao, Caroline S Fox. Genetic associations at 53 loci highlight cell types andbiological pathways relevant for kidney function. Nat Commun. 2016.01; 7; 10023
2. Maki Morishita, Tomoki Muramatsu, Yumiko Suto, Momoki Hirai, Teruaki Konishi, Shin Hayashi, Daichi
Shigemizu, Tatsuhiko Tsunoda, Keiji Moriyama, Johji Inazawa. Chromothripsis-like chromosomal rearrangements induced by ionizing radiation using proton microbeam irradiation system. Oncotarget.
2016.03; 7(9); 10182-10192
3. James Lyons, Kuldip K Paliwal, Abdollah Dehzangi, Rhys Heﬀernan, Tatsuhiko Tsunoda, Alok Sharma.
Protein fold recognition using HMM-HMM alignment and dynamic programming. J. Theor. Biol..
2016.03; 393; 67-74
4. Atsuko Konta, Kouichi Ozaki, Yasuhiko Sakata, Atsushi Takahashi, Takashi Morizono, Shinichiro Suna,
Yoshihiro Onouchi, Tatsuhiko Tsunoda, Michiaki Kubo, Issei Komuro, Yoshinobu Eishi, Toshihiro Tanaka.
A functional SNP in FLT1 increases risk of coronary artery disease in a Japanese population. J. Hum.
Genet.. 2016.05; 61(5); 435-441― 465 ―

Life Scienceand Technology
MedicalGenomics

5. Akihiro Fujimoto, Yukinori Okada, Keith A Boroevich, Tatsuhiko Tsunoda, Hiroaki Taniguchi, Hidewaki
Nakagawa. Systematic analysis of mutation distribution in three dimensional protein structures identiﬁes
cancer driver genes. Sci Rep. 2016.05; 6; 26483
6. Akihiro Fujimoto, Mayuko Furuta, Yasushi Totoki, Tatsuhiko Tsunoda, Mamoru Kato, Yuichi Shiraishi,
Hiroko Tanaka, Hiroaki Taniguchi, Yoshiiku Kawakami, Masaki Ueno, Kunihito Gotoh, Shun-Ichi Ariizumi, Christopher P Wardell, Shinya Hayami, Toru Nakamura, Hiroshi Aikata, Koji Arihiro, Keith
A Boroevich, Tetsuo Abe, Kaoru Nakano, Kazuhiro Maejima, Aya Sasaki-Oku, Ayako Ohsawa, Tetsuo
Shibuya, Hiromi Nakamura, Natsuko Hama, Fumie Hosoda, Yasuhito Arai, Shoko Ohashi, Tomoko Urushidate, Genta Nagae, Shogo Yamamoto, Hiroki Ueda, Kenji Tatsuno, Hidenori Ojima, Nobuyoshi Hiraoka,
Takuji Okusaka, Michiaki Kubo, Shigeru Marubashi, Terumasa Yamada, Satoshi Hirano, Masakazu Yamamoto, Hideki Ohdan, Kazuaki Shimada, Osamu Ishikawa, Hiroki Yamaue, Kazuki Chayama, Satoru
Miyano, Hiroyuki Aburatani, Tatsuhiro Shibata, Hidewaki Nakagawa. Whole-genome mutational landscape and characterization of noncoding and structural mutations in liver cancer. Nat. Genet.. 2016.05;
48(5); 500-509
7. Ikumi Hori, Fuyuki Miya, Kei Ohashi, Yutaka Negishi, Ayako Hattori, Naoki Ando, Nobuhiko Okamoto,
Mitsuhiro Kato, Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh.
Novel splicing mutation in the ASXL3 gene causing Bainbridge-Ropers syndrome. Am. J. Med. Genet.
A. 2016.07; 170(7); 1863-1867
8. Hori I*, Miya F*, Ohashi K, Negishi Y, Hattori A, Ando N, Okamoto N, Kato M, Tsunoda T, Yamasaki M,
Kanemura Y, Kosaki K, Saitoh S.. Novel Splicing Mutation in the ASXL3 gene causing Bainbridge-Ropers
Syndrome. Am. J. Med. Genet.. 2016.07; (A 170); 1863-1867
9. Alok Sharma, Daichi Shigemizu, Keith A Boroevich, Yosvany López, Yoichiro Kamatani, Michiaki Kubo,
Tatsuhiko Tsunoda. Stepwise iterative maximum likelihood clustering approach. BMC Bioinformatics.
2016.08; 17(1); 319
10. Fumihito Nozaki, Takashi Kusunoki, Nobuhiko Okamoto, Yuto Yamamoto, Fuyuki Miya, Tatsuhiko Tsunoda, Kenjiro Kosaki, Tomohiro Kumada, Minoru Shibata, Tatsuya Fujii. ALDH18A1-related cutis laxa
syndrome with cyclic vomiting. Brain Dev.. 2016.08; 38(7); 678-684
11. Nozaki F, Kusunoki T, Okamoto N, Yamamoto Y, Miya F, Tsunoda T, Kosaki K, Kumada T, Shibata
M, Fujii T. . ALDH18A1-related cutis laxa syndrome with cyclic vomiting. Brain Dev.. 2016.08; (38);678-684
12. S-W Chang, C W McDonough, Y Gong, T A Johnson, T Tsunoda, E R Gamazon, M A Perera, A
Takahashi, T Tanaka, M Kubo, C J Pepine, J A Johnson, R M Cooper-DeHoﬀ. Genome-wide association
study identiﬁes pharmacogenomic loci linked with speciﬁc antihypertensive drug treatment and new-onset
diabetes. Pharmacogenomics J.. 2016.09;
13. Nobue Yagihara, Hiroshi Watanabe, Phil Barnett, Laetitia Duboscq-Bidot, Atack C Thomas, Ping Yang,
Seiko Ohno, Kanae Hasegawa, Ryozo Kuwano, Stéphanie Chatel, Richard Redon, Jean-Jacques Schott,Vincent Probst, Tamara T Koopmann, Connie R Bezzina, Arthur A M Wilde, Yukiko Nakano, Takeshi
Aiba, Yoshihiro Miyamoto, Shiro Kamakura, Dawood Darbar, Brian S Donahue, Daichi Shigemizu, Toshihiro Tanaka, Tatsuhiko Tsunoda, Masayoshi Suda, Akinori Sato, Tohru Minamino, Naoto Endo, Wataru
Shimizu, Minoru Horie, Dan M Roden, Naomasa Makita. Variants in the SCN5A Promoter AssociatedWith Various Arrhythmia Phenotypes. J Am Heart Assoc. 2016.09; 5(9);14. Mari Ichikawa, Takeshi Aiba, Seiko Ohno, Daichi Shigemizu, Junichi Ozawa, Keiko Sonoda, Megumi
Fukuyama, Hideki Itoh, Yoshihiro Miyamoto, Tatsuhiko Tsunoda, Takeru Makiyama, Toshihiro Tanaka,
Wataru Shimizu, Minoru Horie. Phenotypic Variability of ANK2 Mutations in Patients With Inherited
Primary Arrhythmia Syndromes. Circ. J.. 2016.11; 80(12); 2435-2442
15. Chisato Shimizu, Hariklia Eleftherohorinou, Victoria J Wright, Jihoon Kim, Martin P Alphonse, JamesC Perry, Rolando Cimaz, David Burgner, Nagib Dahdah, Long T Hoang, Chiea Chuen Khor, Andrea Salgado, Adriana H Tremoulet, Sonia Davila, Taco W Kuijpers, Martin L Hibberd, Todd A Johnson, Atsushi
Takahashi, Tatsuhiko Tsunoda, Michiaki Kubo, Toshihiro Tanaka, Yoshihiro Onouchi, Rae S M Yeung,
Lachlan J M Coin, Michael Levin, Jane C Burns, . Genetic Variation in the SLC8A1 Calcium Signaling
Pathway Is Associated With Susceptibility to Kawasaki Disease and Coronary Artery Abnormalities. Circ
Cardiovasc Genet. 2016.12; 9(6); 559-568― 466 ―

Life Scienceand Technology
MedicalGenomics

16. Ronesh Sharma, Shiu Kumar, Tatsuhiko Tsunoda, Ashwini Patil, Alok Sharma. Predicting MoRFs inprotein sequences using HMM proﬁles. BMC Bioinformatics. 2016.12; 17(Suppl 19); 504
17. Harsh Saini, Sunil Pranit Lal, Vimal Vikash Naidu, Vincel Wince Pickering, Gurmeet Singh, Tatsuhiko
Tsunoda, Alok Sharma. Gene masking - a technique to improve accuracy for cancer classiﬁcation withhigh dimensionality in microarray data. BMC Med Genomics. 2016.12; 9(Suppl 3); 74
18. Makiko Tsutsumi, Setsuri Yokoi, Fuyuki Miya, Masafumi Miyata, Mitsuhiro Kato, Nobuhiko Okamoto,
Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh, Hiroki Kurahashi. Novel compound heterozygous variants in PLK4 identiﬁed in a patient with autosomal recessive
microcephaly and chorioretinopathy. Eur. J. Hum. Genet.. 2016.12; 24(12); 1702-1706
19. Tsutsumi M, Yokoi S, Miya F, Miyata M, Kato M, Okamoto N, Tsunoda T, Yamasaki M, Kanemura
Y, Kosaki K, Saitoh S, Kurahashi H.. Novel compound heterozygous variants in PLK4 identiﬁed in apatient with autosomal recessive microcephaly and chorioretinopathy. Eur. J. Hum. Genet.. 2016.12;(24); 1702-1706[Misc]
1. Daichi Shigemizu, Yukihide Momozawa, Michiaki Kubo, Tatsuhiko Tsunoda. How to select exome enrichment kit Experimental Medicine. 2016.04; 34; 955-960[Conference Activities & Talks]
1. Tatsuhiko Tsunoda. Toward new cancer therapy with genomic/omic analysis. 2016.01.20 Chiba
2. Tatsuhiko Tsunoda. Big data analysis paves the way to personalized medicine. 2016.02.04 Tokyo
3. Tatsuhiko Tsunoda. Disease Multi-Omits Analysis on the Basis of Medical Big Data. CREST Symposium"Elucidation of Life System with Trans-omics" 2016.03.03 Tokyo, Japan
4. Tatsuhiko Tsunoda. Multi-omic big data analysis drives precision medicine. CREST International Symposium on Big Data Application 2016.03.04 Tokyo, Japan
5. Daichi Shigemizu, Yukihide Momozawa, Testuo Abe, Takashi Morizono, Keith A. Boroevich, Sadaaki
Takata, Kyota Ashikawa, Michiaki Kubo, Tatsuhiko Tsunoda. Performance comparison of four commercial
human whole-exome capture platforms. The 13th International Congress of Human Genetics 2016.04.03
Kyoto, Japan
6. Fuyuki Miya, Mitsuhiro Kato, Tadashi Shiohama, Nobuhiko Okamoto, Shinji Saitoh, Mami Yamasaki,
Daichi Shigemizu, Tetsuo Abe, Takashi Morizono, Keith A. Boroevich, Kenjiro Kosaki, Yonehiro Kanemura, Tatsuhiko Tsunoda. A combination of targeted enrichment methodologies for whole-exome sequencing reveals novel pathogenic mutations. The 13th International Congress of Human Genetics 2016.04.03
Kyoto, Japan
7. Nobuhiko Okamoto, Fuyuki Miya, Kenichi Nishioka, Hidenobu Soejima, Tatsuhiko Tsunoda, Mitsuhiro
Kato, Shinji Saitoh, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki. Novel MCA/ID syndrome
with ASH1L mutation. The 13th International Congress of Human Genetics 2016.04.03
8. Ikumi Hori, Fuyuki Miya, Kei Ohashi, Yutaka Negishi, Ayako Hattori, Naoki Ando, Nobuhiko Okamoto,
Mitsuhiro Kato, Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh.
Novel Splicing Mutation in the ASXL3 gene causing Bainbridge-Ropers Syndrome. The 13th International
Congress of Human Genetics 2016.04.03
9. Yonehiro Kanemura, Fuyuki Miya, Tomoko Shofuda, Ema Yoshioka, Daisuke Kanematsu, Kyoko Itoh,
Shinji Fushiki, Takeshi Okinaga, Haruhiko Sago, Rika Kosaki, Kyoko Minagawa, Nobuhiko Okamoto,
Tatsuhiko Tsunoda, Mitsuhiro Kato, Shinji Saitoh, Kenjiro Kosaki, Mami Yamasaki. Novel compound
heterozygous mutations in ISPD gene from two cases of Japanese Walker- Warburg syndrome identi ed bywhole-exome sequencing. The 13th International Congress of Human Genetics 2016.04.03 Kyoto, Japan
10. Nobuhiko Okamoto, Fuyuki Miya, Tatsuhiko Tsunoda, Mitsuhiro Kato, Shinji Saitoh, Mami Yamasaki,
Yonehiro Kanemura, Kenjiro Kosaki. Homozygous ADCY5 mutation causes movement disorder withsevere intellectual disability. The 13th International Congress of Human Genetics 2016.04.03― 467 ―

Life Scienceand Technology
MedicalGenomics

11. Yutaka Negishi, Fuyuki Miya, Ayako Hattori, Naoki Ando, Ikumi Hori, Takao Togawa, Kohei Aoyama,
Kei Ohashi, Shinobu Fukumura, Seiji Mizuno, Ayako Umemura, Yoko Kishimoto, Nobuhiko Okamoto,
Mitsuhiro Kato, Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh.
Combination of genetic and biochemical analyses for the diagnosis of PI3K-AKT-mTOR pathway associated megalencephaly syndromes. The 13th International Congress of Human Genetics 2016.04.03 Kyoto,Japan
12. Fuyuki Miya, Mitsuhiro Kato, Tadashi Shiohama, Nobuhiko Okamoto, Shinji Saitoh, Mami Yamasaki,
Daichi Shigemizu, Tetsuo Abe, Takashi Morizono, Keith A Boroevich, Kenjiro Kosaki, Yonehiro Kanemura, Tatsuhiko Tsunoda. A combination of targeted enrichment methodologies for whole-exome sequencing reveals novel pathogenic mutations. ICHG (The 13th International Congress of Human Genetics)
2016.04.03
13. Nobuhiko Okamoto, Fuyuki Miya, Kenichi Nishioka, Hidenobu Soejima, Tatsuhiko Tsunoda, Mitsuhiro
Kato, Shinji Saitoh, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki. Novel MCA/ID syndrome
with ASH1L mutation. ICHG (The 13th International Congress of Human Genetics) 2016.04.03
14. Ikumi Hori, Fuyuki Miya, Kei Ohashi, Yutaka Negishi, Ayako Hattori, Naoki Ando, Nobuhiko Okamoto,
Mitsuhiro Kato, Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh.
Novel Splicing Mutation in the ASXL3 gene causing Bainbridge-Ropers Syndrome. ICHG (The 13th
International Congress of Human Genetics) 2016.04.03
15. Yonehiro Kanemura, Fuyuki Miya, Tomoko Shofuda, Ema Yoshioka, Daisuke Kanematsu, Kyoko Itoh,
Shinji Fushiki, Takeshi Okinaga, Haruhiko Sago, Rika Kosaki, Kyoko Minagawa, Nobuhiko Okamoto,
Tatsuhiko Tsunoda, Mitsuhiro Kato, Shinji Saitoh, Kenjiro Kosaki, Mami Yamasaki. Novel compound
heterozygous mutations in ISPD gene from two cases of Japanese Walker- Warburg syndrome identi ed
by whole-exome sequencing. ICHG (The 13th International Congress of Human Genetics) 2016.04.03
16. Nobuhiko Okamoto, Fuyuki Miya, Tatsuhiko Tsunoda, Mitsuhiro Kato, Shinji Saitoh, Mami Yamasaki,
Yonehiro Kanemura, Kenjiro Kosaki. Homozygous ADCY5 mutation causes movement disorder withsevere intellectual disability. ICHG (The 13th International Congress of Human Genetics) 2016.04.03
17. Yutaka Negishi, Fuyuki Miya, Ayako Hattori, Naoki Ando, Ikumi Hori, Takao Togawa, Kohei Aoyama,
Kei Ohashi, Shinobu Fukumura, Seiji Mizuno, Ayako Umemura, Yoko Kishimoto, Nobuhiko Okamoto,
Mitsuhiro Kato, Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh.
Combination of genetic and biochemical analyses for the diagnosis of PI3K-AKT-mTOR pathway associated megalencephaly syndromes. ICHG (The 13th International Congress of Human Genetics) 2016.04.03
18. Tatsuhiko Tsunoda. Multi-Omic Analysis on the Basis of Medical Big Data. Kuroda Laboratory Seminar2016.05.11 Tokyo, Japan
19. Atsuko Harada, Fuyuki Miya, Hidetsuna Utsunomiya, Takumi Yamanaka, Yamato Yoshikawa, MichikoMiki, Shofuda Tomoko, Mitsuhiro Kato, Shinji Saitoh, Nobuhiko Okamoto, Kenjiro Kosaki, Tatsuhiko
Tsunoda, Yonehiro Kanemura, Mami Yamasaki. Genetic and biochemical analyses for the diagnosis ofmTOR pathway associated megalencephaly syndromes. The 58th Annual Meeting of the Japanese Societyof Child Neurology 2016.06.02
20. Clinical heterogeneity of genetically conﬁrmed nine patients with Vici syndrome. The 58th Annual Meetingof the Japanese Society of Child Neurology 2016.06.02
21. Genetic analysis in Dandy-Walker malformation. The 58th Annual Meeting of the Japanese Society ofChild Neurology 2016.06.02
22. Saeko Sasaki, Fumihito Nozaki, Nobuhiko Okamoto, Kenjiro Kosaki, Fuyuki Miya, Tatsuhiko Tsunoda,
Tomohiro Kumada, Minoru Shibata, Ikuko Hiejima, Anri Hayashi, Mioko Mori, Kenji Inoue, Tetsuya
Fujii. ALDH18A1 Associated Dermatollis: First Case in Japan. The 58th Annual Meeting of the JapaneseSociety of Child Neurology 2016.06.02
23. Daichi Shigemizu. Current Exome Analysis for Identifying Disease Causing Genes. The 2nd Bioinformatics Agora 2016.07.15 Tokyo, Japan
24. Kumar, S., Sharma, R., Sharma, A., Tsunoda, T.. Decimation Filter with Common Spatial Pattern andFishers Discriminant Analysis for Motor Imagery Classiﬁcation. IEEE World Congress on Computational
Intelligence, IJCNN 2016.07.24 Vancouver, Canada― 468 ―

Life Scienceand Technology
MedicalGenomics

25. Tatsuhiko Tsunoda. Exploring etiologies, sub-classiﬁcation, and risk prediction of diseases based on bigdata analysis of clinical and whole omics data in medicine. CREST Big Data Field Joint Symposium2016.08.05 Tokyo, Japan
26. Yosvany López, Alok Sharma, Tatsuhiko Tsunoda. Survey of Highly Mutated Transcription Factor Binding Sites in Human Cancers. 15th International Conference on Bioinformatics (InCoB 2016) 2016.09.21
Singapore
27. Tatsuhiko Tsunoda. Precision Medicine on the Basis of Medical Big Data Analysis. Symposium of theJapan Gene Therapy Association Symposium "Foundations of Genetic Statistics and Clinical Application"2016.10.07 Tokyo, Japan
28. Daichi Shigemizu, Fuyuki Miya, Akihiro Fujimoto, Keith A. Boroevich, Shujiro Okuda, Tatsuhiko Tsunoda. Development of a long indel detection method using the realignment of the misaligned reads. The
American Society of Human Genetics 2016.10.18 Vancouver, Canada
29. Makiko Tsutsumi, Setsuri Yokoi, Fuyuki Miya, Masafumi Miyata, Mitsuhiro Kato, Nobuhiko Okamoto,
Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh, Hiroki Kurahashi. Missense mutations in the PLK4 gene identiﬁed in a patient with autosomal recessive microcephaly
and chorioretinopathy. The American Society of Human Genetics 2016.10.18
30. Makiko Tsutsumi, Setsuri Yokoi, Fuyuki Miya, Masafumi Miyata, Mitsuhiro Kato, Nobuhiko Okamoto,
Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh, Hiroki Kurahashi. Missense mutations in the PLK4 gene identiﬁed in a patient with autosomal recessive microcephaly
and chorioretinopathy. The 66th Annual Meeting of the American Society of Human Genetics 2016.10.18
31. Tatsuhiko Tsunoda. Trans-omic analysis strategy for precision medicine. The 26th Hot Spring Harbor International Symposium - Trans-omics: New Approaches in Biology and Medicine 2016 2016.11.02
Fukuoka, Japan
32. Daichi Shigemizu. Trend of exome analysis for identifying disease causing gene mutation. Science Research
Foundation Research A Symposium (I) Large-scale data analysis and modeling to understand complex
life phenomena 2016.11.07 Kurume
33. Artem Lysenko, Piotr J. Kamola, Keith A. Boroevich, Tatsuhiko Tsunoda. Strategies for discovering
disease-associated modules in integrated biological networks. 9th Annual RECOMB/ISCB Conference on
Regulatory & Systems Genomics 2016.11.07
34. Artem Lysenko, Piotr J. Kamola, Keith A. Boroevich, Tatsuhiko Tsunoda. Comparative evaluation of
approaches for module identiﬁcation in biological networks. 9th Annual RECOMB/ISCB Conference on
Regulatory & Systems Genomics 2016.11.07
35. Chikahiko Numakura, Kazuyuki Nakamura, Fuyuki Miya, Jun Mizota, Tetsuo Mitsui, Mitsuhiro Kato.
Two siblings of skeletal ciliopathy with restrictive ventilatory impairment harbouring mutations in C21orf2
gene. The 50th Annual Meeting of the Japanese Society for Pediatric Endocrinology and the 9th Biennial
Scientiﬁc Meeting of the Asia Paciﬁc Pediatric Endocrinology Society 2016.11.16 Tokyo, Japan
36. Tatsuhiko Tsunoda. Exploring etiologies, sub-classiﬁcation, and risk prediction of diseases based on bigdata analysis of clinical and whole omics data in medicine. CREST Big Data Fields Joint Meeting2016.11.29 Tokyo, Japan
37. Kumar, S., Sharma, A., Mamun, K., Tsunoda, T.. A deep learning approach for motor imagery EEG
signal classiﬁcation. IEEE Asia-Paciﬁc World Congress on Computer Science 2016.12.04
38. Makiko Tsutsumi, Setsuri Yokoi, Fuyuki Miya, Masafumi Miyata, Mitsuhiro Kato, Nobuhiko Okamoto,
Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh, Hiroki Kurahashi. The eﬀect of novel missense mutation of PLK4 gene identiﬁed in microcephaly patients on central
body duplication. The 39th Annual Meeting of the Japan Society of Pediatric Genetics 2016.12.09 Tokyo,
Japan
39. Shinji Saitoh, Izumi Hori, Fuyuki Miya, Daisuke Ieda, Yutaka Negishi, Nobuhiko Okamoto, Ayako Hattori, Mitsuhiro Kato, Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Hiroki
Kurahashi. Two cases of Bainbridge-Ropers syndrome in which ASXL3 gene mutation was identiﬁed. The
39th Annual Meeting of Japan Society of Pediatric Genetics 2016.12.09 Tokyo, Japan― 469 ―

Life Scienceand Technology
MedicalGenomics[Patents]
1. METHOD FOR SELECTING IPS CELL CLONE, AND METHOD FOR SELECTING GENE USED IN
METHOD FOR SELECTING SAME, Announcement Number：WO 2012/115270[Works]1. HML: Tool to perform Hierarchical Maximum Likelihood (HML) Clustering, Software, 2016.03
2. SIML: Matlab package of SIML clustering approach, Software, 2016.08[Awards & Honors]
1. Dream Challenges Certiﬁcate of Best Performance - Disease Module Identiﬁcation DREAM Challenge
Sub-Challenge 2, DREAM Challenge, 2016.11― 470 ―

Life
LifeScience
Science andand Technology
Technology

Structural Biology

Professor Nobutoshi ITO
Associate Professor Teikichi IKURA
Assistant Professor Nobutaka NUMOTO(1)

Outline

The advance of genome science and proteomic analysis has produced a large amount of information about the
primary structure of proteins and their spatial and temporal distributions. On the other hand, most of theproteins only function when they take certain three dimensional structures. As obviously seen in so-called priondiseases, proteins which are chemically correct but structurally incorrect not only fail to function properly but
also can harm cells. Our laboratory aims to understand the function of biological macromolecules at atomic
level through structure analysis and other methods of physical chemistry, in the hope that accumulation of suchknowledge will eventually lead to development of drugs. We are also involved in providing database of such
structural data to scientists through the activities of Protein Data Bank Japan.
(2)

Research

Collaborating with groups within and outside of the university, we are engaged in various research projects
including;1) Physicochemical analysis on the mechanism of the signal transduction for activation of T cells
2) Structural analyses of B-cell coreceptors3) Molecular basis of suppression of HIV-1
4) Structural basis of giant hemoglobins
5) Analysis of interactions between tau protein and Pin1
6) Structure based drug design for protein kinases7) Structural analyses of potential drug targets such as nuclear receptors
8) Improvement in Protein Data Bank

(3)

Lectures & Courses

The students learn theoretical basis of structure determination, mainly X-ray crystallography, of proteins andother biological macromolecules. Recent advance in structural biology is also discussed in seminar. Students
learn lab techniques related to large-scale production, puriﬁcation and crystallization of protein samples. They
also learn computational methods to determine and reﬁne crystal structures.

(4)

Publications[Original Articles]
1. Isao Kii, Yuto Sumida, Toshiyasu Goto, Rie Sonamoto, Yukiko Okuno, Suguru Yoshida, Tomoe KatoSumida, Yuka Koike, Minako Abe, Yosuke Nonaka, Teikichi Ikura, Nobutoshi Ito, Hiroshi Shibuya,― 471 ―

Life Science
Science andand Technology
Technology
Life

Takamitsu Hosoya, Masatoshi Hagiwara. Selective inhibition of the kinase DYRK1A by targeting its
folding process. Nat Commun. 2016; 7; 11391
2. Manjiri R Kulkarni, Nobutaka Numoto, Nobutoshi Ito, Yutaka Kuroda. Modeling and experimental assessment of a buried Leu-Ile mutation in dengue envelope domain III. Biochem. Biophys. Res. Commun..
2016.02; 471(1); 163-168
3. Akira Nakamura, Jun Ohtsuka, Tatsuki Kashiwagi, Nobutaka Numoto, Noriyuki Hirota, Takahiro Ode,
Hidehiko Okada, Koji Nagata, Motosuke Kiyohara, Ei-Ichiro Suzuki, Akiko Kita, Hitoshi Wada, Masaru
Tanokura. In-situ and real-time growth observation of high-quality protein crystals under quasi-microgravity
on earth. Sci Rep. 2016.02; 6; 22127
4. Tomomi Imamura, Kyota Fujita, Kazuhiko Tagawa, Teikichi Ikura, Xigui Chen, Hidenori Homma, Takuya
Tamura, Ying Mao, Juliana Bosso Taniguchi, Kazumi Motoki, Makoto Nakabayashi, Nobutoshi Ito,
Kazunori Yamada, Kentaro Tomii, Hideyuki Okano, Julia Kaye, Steven Finkbeiner, Hitoshi Okazawa.
Identiﬁcation of hepta-histidine as a candidate drug for Huntington’s disease by in silico-in vitro- in
vivo-integrated screens of chemical libraries. Sci Rep. 2016.09; 6; 33861
5. Chizuru Akatsu, Kenro Shinagawa, Nobutaka Numoto, Zhihong Liu, Ayse Konuskan Ucar, Mohammad
Aslam, Shirly Phoon, Takahiro Adachi, Koji Furukawa, Nobutoshi Ito, Takeshi Tsubata. CD72 negatively
regulates B lymphocyte responses to the lupus-related endogenous toll-like receptor 7 ligand Sm/RNP. J.
Exp. Med.. 2016.11; 213(12); 2691-2706[Misc]
1. Teikichi Ikura, Nobutoshi Ito. Crystal Structure of the Vitamin D Receptor Ligand-Binding Domain with
Lithocholic Acids. Vitam. Horm.. 2016; 100; 117-136[Conference Activities & Talks]
1. Nobutaka Numoto, Taro Nakagawa, Nobutoshi Ito, Yoshihiro Fukumori, Kunio Miki. Analysis of theoxygen-dissociation intermediate states of the giant hemoglobin. The 16th Annual Meeting of the Protein
Science Society of Japan 2016.06 Fukuoka
2. Teikichi Ikura, Nobutoshi Ito. Analysis of catalytic mechanism of a protease derived from Pin1. The 16th
Annual Meeting of the Protein Science Society of Japan 2016.06.08 Fukuoka
3. Nobutaka Numoto, Taro Nakagawa, Nobutoshi Ito, Yoshihiro Fukumori, Kunio Miki. Oxygen-dissociationintermediate of the giant hemoglobin from Oligobrachia mashikoi. Annual Meeting 2016 and General
Assembly of the Crystallographic Society of Japan 2016.11 Ibaraki
4. Nobutaka Numoto, Taro Nakagawa, Nobutoshi Ito, Yoshihiro Fukumori, Kunio Miki. Crystallographic
and spectroscopic analysis of the oxygen-dissociation intermediate of the giant hemoglobin. The 54th
Annual Meeting of the Biophysical Society of Japan 2016.11 Ibaraki
5. Satomi Inaba, Nobutaka Numoto, Shuhei Ogawa, Hisayuki Morii, Teikichi Ikura, Ryo Abe, Nobutoshi Ito,
Masayuki Oda. Structural and thermodynamic analysis of interactions between CD28 and SH2 domains.
The 54th Annual Meeting of the Biophysical Society of Japan 2016.11.25 Tsukuba
6. Teikichi Ikura, Nobutoshi Ito. Activity of a protease derived from Pin1 for tau protein. The 54th Annual
Meeting of the Biophysical Society of Japan 2016.11.26 Tsukuba― 472 ―

Life
LifeScience
Science andand Technology
Technology

Neuroscience

Professor Kohichi Tanaka
Associate Professor Tomomi Aida
Assistant Professor Saeko Ishida
Assistant Professor Yuichi Hiraoka (2015/5/1 )Graduate Student (doctor course)
Zhao Zhuoyang
Kaori Sugiyama
Takehisa Handa
Graduate Student (master course)
Mina Kusunose
Moeko Tanaka
Haruka Takigawa
Hiroshi Ogawa
Technical Staﬀ
Harumi Ishikubo
Secretary
Satomi Ohno
(1)

OutlineThe ﬁnal goal of our research is to understand molecular, cellular, and neuronal ensemble mechanisms underlying
higher order brain functions including learning and memory. For that purpose, we combine molecular genetics,
physiological and behavioral methods. The laboratory also studies the mechanism that underlies neuronal cell
death and regeneration.
(2)

Research

1. Functions of glutamate transporters in the brain
Glutamate is a major excitatory neurotransmitter and plays an important role in neuronal plasticity and neurotoxicity in the central nervous system. Glutamate transport proteins provide the mechanism by which synaptically released glutamate is inactivated and kept below toxic levels in the extracellular space. By now, ﬁve
subtypes of high-aﬃnity glutamate transporters have been identiﬁed in the mammalian brain. Our lab studies
the physiological and pathological roles of glutamate transporter subtypes using subtype-speciﬁc knockout mice.
Clinical phenotypes of spinocerebellar ataxia type-5 (SCA5) and spectrin-associated autosomal recessive cerebellar ataxia type-1 (SPARCA1) are mirrored in mice lacking β-III spectrin (β-III-/-). One function of β-III
spectrin is the stabilization of the Purkinje cell-speciﬁc glutamate transporter EAAT4 at the plasma membrane.
In β-III-/- mice EAAT4 levels are reduced from an early age. In contrast levels of the glutamate transporter
GLAST, expressed in Bergmann glia, only fall progressively from 3 months onwards. Here we elucidated theroles of these two glutamate transporters in cerebellar pathogenesis mediated through loss of β-III spectrin
function by studying EAAT4 and GLAST knockout mice as well as crosses of both with β-III-/- mice. Our― 473 ―

Life Science
Science andand Technology
Technology
Lifedata demonstrate that EAAT4 loss, but not abnormal AMPA receptor composition, in young β-III-/- mice
underlies early Purkinje cell hyper-excitability and that subsequent loss of GLAST, superimposed on the earlier
deﬁciency of EAAT4, is responsible for Purkinje cell loss and progression of motor deﬁcits. Yet the loss of
GLAST appears to be independent of EAAT4 loss, highlighting that other aspects of Purkinje cell dysfunction
underpin the pathogenic loss of GLAST. Finally, our results demonstrate that Purkinje cells in the posterior
cerebellum of β-III-/- mice are most susceptible to the combined loss of EAAT4 and GLAST, with degenerationof proximal dendrites, the site of climbing ﬁbre innervation, most pronounced. This highlights the necessity foreﬃcient glutamate clearance from these regions and identiﬁes dysregulation of glutamatergic neurotransmission
particularly within the posterior cerebellum as a key mechanism in SCA5 and SPARCA1 pathogenesis.
We investigated the cytoprotective eﬀect of geranylgeranylacetone (GGA) on RGCs degeneration using a normal
tension glaucoma (NTG) mouse model, which lacks GLAST. Three-week-old GLAST+/− mice were given oral
administration of GGA at 100, 300, or 600 mg/kg/day or vehicle alone, and littermate control mice were given
vehicle alone for 14 days, respectively. The number of RGCs of GLAST+/− mice signiﬁcantly decreased, as
compared to that of control mice. RGC loss was signiﬁcantly suppressed by administration of GGA at 600
mg/kg/day, compared with vehicle alone. Following GGA administration, HSP70 was signiﬁcantly upregulated
together with reduction in the activities of caspase-9 and -3. Our studies highlight HSP70 induction in theretina is available to suppress RGC degeneration, and thus GGA may be applicable for NTG as a promisingtherapy.
2. Development of genome editing technologies
Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing longhomology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Casbased precise integration into the target chromosome (PITCh) system for a gene cassette knock-in without longhomology arms mediated by microhomology-mediated end-joining.
Here, we identiﬁed exonuclease 1 (Exo1) as an enhancer for PITCh in human cells. By combining the Exo1
and PITCh-directed donor vectors, we achieved convenient one-step knock-in of gene cassettes and ﬂoxed allele
both in human cells and mouse zygotes. Our results provide a technical platform for high-throughput knock-in.

(3)

Education

Goals/Outline:
Students should generate genetically modiﬁed animals to comprehensively understand the cognitive mechanisms
at the level of molecule to behavior. Then, students should analyze cognitive deﬁcits of mutant animals andthose molecular mechanisms.
Available programs:
Participation in the ongoing research project; as needed
Training for cell biology: ﬁve times a year 13:00 – 16:00
Experiment:
1. Gene cloning and generation of targeting vector.
2. Generation of genetically modiﬁed mice
3. Behavioral analysis of the mice
4. Morphological analysis of central nervous systems.

(4)

Lectures & Courses

The aim of this practice is to learn molecular biological, anatomical, electrophysiological and psychologicalapproaches to elucidate the mechanism of cognition. Moreover, based on previous case reports of cognitivedeﬁcits, students should plan and discuss what kinds of the researches are possible and meaningful to elucidate
the pathology of these diseases, leading to unveil the mechanism of cognition.

― 474 ―

Life
LifeScience
Science andand Technology
Technology(5)

Publications[Original Articles]
1. Emma M Perkins, Daumante Suminaite, Yvonne L Clarkson, Sin Kwan Lee, Alastair R Lyndon, Jeﬀrey
D Rothstein, David J A Wyllie, Kohichi Tanaka, Mandy Jackson. Posterior cerebellar Purkinje cells in an
SCA5/SPARCA1 mouse model are especially vulnerable to the synergistic eﬀect of loss of β-III spectrin
and GLAST. Hum. Mol. Genet.. 2016.10; 25(20); 4448-4461
2. Zhenyu Dong, Yasuhiro Shinmei, Yoko Dong, Saori Inafuku, Junichi Fukuhara, Ryo Ando, Nobuyoshi
Kitaichi, Atsuhiro Kanda, Kohichi Tanaka, Kousuke Noda, Takayuki Harada, Shinki Chin, Susumu Ishida.
Eﬀect of geranylgeranylacetone on the protection of retinal ganglion cells in a mouse model of normal
tension glaucoma. Heliyon. 2016.10; 2(10); e00191
3. Tomomi Aida, Shota Nakade, Tetsushi Sakuma, Yayoi Izu, Ayu Oishi, Keiji Mochida, Harumi Ishikubo,
Takako Usami, Hidenori Aizawa, Takashi Yamamoto, Kohichi Tanaka. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ. BMC Genomics. 2016.11; 17(1); 979― 475 ―

Life Science and Technology

Life Science and Technology

Epigenetic Epidemiology

Professor: Masaaki MURAMATSU
Associate Professor : Noriko SATO
Assistant Professor : Chihiro Imai
Adjunct Instructor : Katsuko SUDO, Jun-ichi TAGUCHI
Graduate Student: Kaung Si Thu,
Khin Thet Thet Zaw, Yuko Maeda, Fujitani,
Tay Zar Kyaw, Tadaaki Katsuta, Shilpa Pavethynath
Maidina Abudushataer, Ake Ko Ko Minn
Norihiko Satake, Kenji Suzuki, Hirokazu Sakamoto
Yuiri Tsubota
Research Resident: Zong Yuan
(1)

OutlineMany common chronic diseases are multifactorial in that they are caused by multiple genetic and environmental
factors. By applying the technology and information of human genome to epidemiological studies, we aim toclarify the role of genetic polymorphisms, epigenetic changes, as well as their interaction with environmental
factors, which may contribute to the development of these diseases.
(2)

Research

Our research subjects are as follows.
1. Gene-environment interaction that aﬀects the onset of metabolic syndrome and its related phenotypes.
2. Genetic factors that aﬀect the severity of pathological atherosclerosis.
3. Responder vs non-responder of prodrugs and polymorphisms of drug metabolizing enzymes.
4. Severe cutaneous adverse response (Stevens-Jhonson s Syndrome) and HLA genotypes.
5. The role of epigenetic regulation and fetal programming in common diseases.
6. Likelihood ratio based integrated personal risk assessment of type 2 diabetes.

(3)

Education

Masaaki Muramatsu:Holistic Study of Disease Prevention Ⅰ
Masaaki Muramatsu:Environmental/Social Health
Masaaki Muramatsu:Negotiation and Debate in English
Noriko Sato, Masaaki Muramatsu: Bioscience I
Noriko Sato: Molecular and Cellular Biology
Noriko Sato: Introduction to Human Molecular Genetics― 476 ―

Life Science and Technology

Life Science and Technology(4)

Lectures & Courses

We focus on common diseases such as diabetes, hypertension, obesity, metabolic syndrome, and atherosclerosis
which are caused by multiple genetic and environmental factors, and aim to decipher these factors as well as
their interactions by applying the technology and information of human genome to epidemiology. Our goal is
not only to identify disease genes and polymorphisms but also to elucidate gene-environment interactions that
contribute to the onset and progression of the diseases. Epigenetic changes in common diseases are also in
our scope. A new project has been started to study methods for educating genome-based health literacy byemploying information generated from personal genome sequences(5)

Publications[Original Articles]
1. Kumpei Tanisawa, Yasumichi Arai, Nobuyoshi Hirose, Hiroshi Shimokata, Yoshiji Yamada, Hisashi Kawai,
Motonaga Kojima, Shuichi Obuchi, Hirohiko Hirano, Hideyo Yoshida, Hiroyuki Suzuki, Yoshinori Fujiwara, Kazushige Ihara, Maki Sugaya, Tomio Arai, Seijiro Mori, Motoji Sawabe, Noriko Sato, Masaaki
Muramatsu, Mitsuru Higuchi, Yao-Wen Liu, Qing-Peng Kong, Masashi Tanaka. Exome-wide Association
Study Identiﬁes CLEC3B Missense Variant p.S106G as Being Associated With Extreme Longevity in East
Asian Populations. J. Gerontol. A Biol. Sci. Med. Sci.. 2016.05;
2. Kaung Si Thu, Noriko Sato, Shinobu Ikeda, Makiko Naka-Mieno, Tomio Arai, Seijiro Mori, Motoji Sawabe,
Masaaki Muramatsu, Masashi Tanaka. Association of polymorphisms of the transporter associated with
antigen processing (TAP2) gene with pulmonary tuberculosis in an elderly Japanese population. APMIS.
2016.08; 124(8); 675-680
3. Khin Thet Thet Zaw, Noriko Sato, Shinobu Ikeda, Kaung Si Thu, Makiko Naka Mieno, Tomio Arai, Seijiro
Mori, Tetsushi Furukawa, Tetsuo Sasano, Motoji Sawabe, Masashi Tanaka, Masaaki Muramatsu. Association of ZFHX3 gene variation with atrial ﬁbrillation, cerebral infarction, and lung thromboembolism:
An autopsy study. J Cardiol. 2016.12;
4. Yuko Maeda, Noriko Sato, Makiko Naka-Mieno, Seijiro Mori, Tomio Arai, Masashi Tanaka, Masaaki
Muramatsu, Motoji Sawabe. Association of non-synonymous variants in WIPF3 and LIPA genes withabdominal aortic aneurysm: an autopsy study. J Geriatr Cardiol. 2016.12; 13(12); 960-967[Conference Activities & Talks]
1. Noriko Sato, et al.. Cohort proﬁle: Birth Cohort- Gene and Environment Interaction Study of TMDU
(BC-GENIST). The 5th Annual Meeting of the Japan Society for Developmental Origins of Health andDisease 2016.07.23 Tokyo
2. Noriko Sato. To understand the mechanisms by which metabolic disease develop due to antenatal nutritional environment . The 63rd meeting for the Japanese society of nutrition and dietetics 2016.09.08― 477 ―

Life Science and Technology

RIKEN Molecular and Chemical Somatology

Visiting Professor Soichi Kojima
Visiting Professor

Mikiko Sodeoka

Visiting Professor Nobumoto Watanabe
Visiting Professor Yoshiki Yamaguchi
Visiting Professor Takashi Saito
Visiting Professor Motomasa Tanaka
Visiting Lecturer

Takeshi Nakano

Visiting Lecturer

Kosuke Dodo

Visiting Lecturer

Yutaka Furutani

Visiting Lecturer

Qin Xian-Yang

Visiting Lecturer

Akiko Tane

Visiting Lecturer

Ambara R. Pradipta

Visiting Lecturer

Masamichi Nagae

Visiting Lecturer

Nobuhiko Miyasaka

Visiting Lecturer

Tetsuya Koide

Graduate Students
D3

Sayoko Yamasaki
Hari Prasad Dulal
Ryo Fukazawa

(1)

D2

Kruthi Sharamjeet Suvarna

D1

Mengqian Li

M2

Sho Fukushima

Research

Molecular and Chemical Somatology is an interdisciplinary field to understand basis of
Bioorganic Chemistry, Chemical Biology, Structural Biology, Molecular Immunology,
and Molecular Neuroscience as well as their applications to Medicine and Biology bydealing with variety of molecules that regulate cellular functions including low
molecular weight organic compounds, proteins, sugars, and hormones.

Students willhear and discuss about outlines and/or latest topics on discovery, structure, synthesis,
biology, and management of these key molecules/factors, and deepen theirunderstanding of this new study field.

― 478 ―

Life Science and Technology(2)

Education１）Synthetic Organic Chemistry・Design and synthesis of bioactive molecules based on synthetic organic chemistry
and chemical biology research.
２）Chemical Biology
・Discovery, target identification and analyses of mechanism of action of bioactive
compounds that regulate biological function.
３）Molecular Cellular Pathology
・Clarification of pathogenesis of diseases at molecular and cellular levels utilizing
bioprobes.
４）Structural Biology
・Analyses of structure and functions of bioactive glycoproteins and related proteins５）Molecular Immunology
・Regulatory mechanisms for the lymphocyte activation and immune responses.
６）Molecular Neuropathology
・Molecular basis of psychiatric diseases and neurodegenerative disorders(3)

Publications[Original Articles]
1. Ando, J., Asanuma, M., Dodo, K., Yamakoshi, H., Kawata, S.; Fujita, K., Sodeoka,
M. Alkyne-Tag SERS Screening and Identification of Small-Molecule-Binding
Sites in Protein. J. Am. Chem. Soc. 138: 13901-13910, 2016.
2. Subedi, A., Futamura, Y., Nishi, A., Ryo, A., Watanabe, N., and Osada, H.
Highthroughput screening identifies artesunate as selective inhibitor of cancer
stemness. Involvement of mitochondrial metabolism. Biochem Biophys Res
Commun. 477, 737-742 (2016)
3. Dulal HP, Nagae M, Ikeda A, Morita-Matsumoto K, Adachi Y, Ohno N, Yamaguchi
Y, Enhancement of solubility and yield of a β-glucan receptor Dectin-1 C-type
lectin-like domain in Escherichia coli with a solubility-enhancement tag. Protein
Expr. Purif., 123, 97-104 (2016)
4. Takeuchi, A., Badr, M.E.S.G., Miyauchi, K., Ishihara C., Onishi R., Guo Z., Sasaki,T., Ike H., Takumi, A., Tsuji N.M., Murakami, Y., Katakai, T. Kubo M. and Saito,
T.: CRTAM determines the CD4+ cytotoxic T lymphocyte lineage. J. Exp.Med.
213:123-138, 2016.

― 479 ―

Life Science and Technology

5. Hashimoto-Tane A., Sakuma M., Ike H., Yokosuka T., Kimura Y., Ohara O. andSaito T.: Micro adhesion-ring surrounding TCR microclusters are essential for T
cell activation. J. Exp.Med. 213:1609-1625, 2016.
6. Shrestha, R., Shrestha, R.,Qin, X-Y., Kou, T.-F., Oshima, Y., Iwatani, S., Teraoka,
R., Fujii, K., Hara, M., Li, M., Takahashi-Nakaguchi, A., Chibana, H., Lu, J., Cai,
M., Kajiwara, S., and Kojima, S. Fungus-derived hydroxyl radicals kill hepatic
cells by enhancing nuclear transgluminase. Sci Rep. in press.,2017[Review Articles]
1. Hirai, G., Asanuma, M., Tsuchiya, A., Sodeoka, M. Development of dual-specificity
protein phosphatases inhibitors based on focused library approach: Modificationof a core structure and unique inhibition mechanism. J. Synth. Org. Chem., Japan.
74: 532-540, 2016.
2. Watanabe, N., Osada, H. Small molecules that target phosphorylation dependent
protein-protein interaction. Bioorg Med. Chem. 24, 3264-3254 (2016)
3. Hashimoto-Tane, A and Saito, T.: Dynamic regulation of TCR-microcluster andmicrosynapse

for

T

cell

activation. Front.

Immunol

7:255,

2016,

doi:

10.3389/fimmu.2016.00255
4. Tatsukawa, H., Furutani, Y., Hitomi, K., and Kojima, S. Transglutaminase 2, has
opposing roles in the regulation of cellular functions as well as cell growth anddeath. (Review) Cell Death & Disease. 7:e2244, 2016

[Books]
1. Furutani, Y. and Kojima, S. Control of TG functions depending on theirlocalization. In Transglutaminase – multiple functional modifiers and targets for
new drug discovery (Hitomi, K., Kojima, S., and Fesus, L. eds) Springer Tokyo,
43-62, 2016

[Conference Activities & Talks]
1. Sodeoka, M. “Enantioselective Reactions of α-Ketoesters.“ 27th International
Conference on Organometallic Chemistry, Melbourne, Australia, 2016.
2. Saito T. Hashimoto-Tane A. “Microsynapse composed of micro-adhesion ringsurrounding TCR microcluster is essential for T cell activation” EMBO conference(Siena, Italy) September, 2016
3. Saito T. “Dynamic Regulation of T cell Activation at Immune Synapse” POSTEC
Symposium (Pohang, Korea) July, 2016

― 480 ―

Life Science and Technology

4. Kojima, S. “Molecular mechanism by which acyclic retinoid induces nuclear
localization of transglutaminase 2 resulting in selective cell death in liver cancer
stem cells” 3rd International Federation of American Societies for Experimental
Biology (FASEB) Conference on Retinoids, West Palm Beach, FL, USA, June,2016.

― 481 ―

Life Science and Technology

Life Science and Technology

Metallic Biomaterials

Takao HANAWA Prof
Yusuke TSUTSUMI Assoc Prof
Maki ASHIDA Assist Prof
Peng CHEN Assist Prof
Hisashi DOI Assist Prof
Tomoko SETOGUCHI Secretary(1)

Outline1. Bio-functionilization of metals with surface modiﬁcation
Bio-functionalization of metals is investigated with surface treatment techniques, such as molecule immobilization and anodic oxidation. These surface treatments make it possible to inhibit protein adsorption, platelet
adhesion, and bioﬁlm formation, and to enhance wear resistance and hard-tissue compatibility.
2. Development of novel alloys and porous composites for biomedical applications
Novel alloy systems for biomedical applications are designed from the viewpoints of mechanical properties andbiocompatibility. Co-Cr-Mo alloys having high strength and ductility for dental applications are developed.
The porous alloys having low Young s modulus are obtained with selective laser melting technique.
3. Development of Zr-based alloys for minimizing MRI artifacts
Zr-based alloys with low magnetic susceptibility, high strength and corrosion resistance are investigated forminimizing MRI artifact by controlling their microstructure and constituent phase for aneurysm clips, artiﬁcial
joints, and dental implants, etc.
4. Eﬀort to minimalize metal allergyCountermeasure techniques for metal ion release from metallic biomaterials which causes metal allergy are
investigated. Novel reagents of patch testing for the detection of sensitization to metal ions are developed.
(2)

Lectures & Courses

Metallic biomaterials play an important role as medical devices. Our laboratory mainly deals with eﬀects ofcrystal structure, process, and thermal treatment on mechanical properties (e.g. strength or toughness). We
also focus on structure and property of nanometer-scaled surface phenomena: Formation of living tissue on
metals, especially, reactions between biomolecules or cells and metals, changes in surface oxide layers in living
tissues, and electrochemical property of metallic biomaterials. The aim of the education is perfect understandingof metallic biomaterials, enabling students to select a proper material for medical treatments or researches.
(3)

Publications[Original Articles]
1. Park JW, Kang DG, Hanawa T. New bone formation induced by surface strontium-modiﬁed ceramic bone
graft substitute Oral Dis.. 2016.01; 22(1); 53-61
2. Kajima Y, Takaichi A, Yasue T, Doi H, Takahashi H, Hanawa T, Wakabayashi N. Evaluation of the shear
bond strength of dental porcelain and the low magnetic susceptibility Zr-14Nb alloy J. Mech. Behav.
Biomed. Mater.. 2016.01; 53; 131-141― 482 ―

Life Science and Technology

Life Science and Technology

3. Tsukamoto M, Kawa T, Shinonaga T, Chen P, Nagai A, Hanawa T. Cell spreading on titanium periodic
nanostructures with periods of 200, 300 and 600 nm produced by femtosecond laser irradiation Appl.
Phys. A.. 2016.02; 122(2); 120
4. Tsutsumi Y, Niinomi M, Nakai M, Shimabukuro M, Ashida M, Chen P, Doi H, Hanawa T. Electrochemical surface treatment of a β-titanium alloy to realize an antibacterial property and bioactivity Metals.
2016.03; 6(4); 76
5. Chen P, Nagai A, Tsutsumi Y, Ashida M, Doi H, Hanawa T . Diﬀerences in the calciﬁcation of preosteoblast
cultured on sputter-deposited titanium, zirconium, and gold J Biomed Mater Res A. 2016.03; 104(3); 639651
6. Fukuhara Y, Kyuzo M, Tsutsumi Y, Nagai A, Chen P, Hanawa, T. Phospholipid polymer electrodepositedon titanium inhibits platelet adhesion J Biomed Mater Res B Appl Biomater. 2016.04; 104(3); 554-560
7. Lee TJ, Ueno T, Nomura N, Wakabayashi N, Hanawa T. Titanium-zirconium binary alloy as dental
implant material: analysis of the inﬂuence of compositional change on mechanical properties and in vitro
biologic response Int. J. Oral Maxillofac.. 2016.05; 31(3); 547-554
8. Kajima Y, Takaichi A, Nakamoto T, Kimura T, Yogo Y, Ashida M, Doi H, Nomura N, Takahashi H,
Hanawa T, Wakabayashi N. Fatigue strength of Co-Cr-Mo alloy clasps prepared by selective laser melting
J. Mech. Behav. Biomed. Mater.. 2016.06; 59; 446-458
9. Sato M, Chen P, Tsutsumi Y, Shiota M, Hanawa T, Kasugai S . Eﬀect of strontium ions on calciﬁcationof preosteoblasts cultured on porous calcium- and phosphate-containing titanium oxide layers formed by
micro-arc oxidation Dental Materials Journal. 2016.07; 35(4); 627-634
10. Chen P, Ashida M, Doi H, Tsutsumi Y, Horita Z, Hanawa T . Cytocompatibility of Ti–6Al–7Nb throughhigh-pressure torsion processing Mater Trans. 2016.11; 57(12); 2020-2025
11. Kondo R, Nomura N, Doi H, Matsumoto H, Tsutsumi Y, Hanawa T. Eﬀect of heat treatment and thefabrication process on mechanical properties of Zr-14Nb alloy Mater. Trans. . 2016.12; 57(12); 2060-2064
12. Tsutsumi Y, Ashida M, Nakahara K, Serizawa A, Doi H, Grandini CR, Rocha LA, Hanawa T. Micro arc
oxidation of Ti-15Zr-7.5Mo alloy Mater. Trans.. 2016.12; 57(12); 2015-2019
13. Tsustumi Y, Doi H, Nomura N, Ashida M, Chen P, Kawasaki A, Hanawa T. Surface composition andcorrosion resistance of Co-Cr alloys containing high chromium Mater. Trans.. 2016.12; 57(12); 2033-2040[Misc]
1. Hanawa T. Electrodeposition of calcium phosphates, oxides, and molecules to achieve Biocompatibility ofmetals Ref. Module Chem. Mol. Sci. Chem. Eng. . 2016.06;[Conference Activities & Talks]
1. Hanawa T. Future of metalic biomaterials. 10th World Biomaterials Congress (WBC2016) 2016.05.17
Montreal,Canada
2. Hanawa T, Nomura N, Nakano T. Advanced researches of additive manufacturing for medicine in Japan.
10th World Biomaterials Congress (WBC2016) 2016.05.17 Montreal,Canada
3. Yoshihara C, Ueno T, Chen P, Tsutsumi Y, Hanawa T, Wakabayashi N. Inverse behavior between osteoblasts and ﬁbroblasts on carbon-coated titanium surfaces. 10th World Biomaterials Congress (WBC2016)2016.05.17 Montreal,Canada
4. Akimoto T, Ueno T, Tsutsumi Y, Doi H, Hanawa T, Wakabayashi N. The Corrosion Resistance of TiZr Binary Alloy with Compositional Change. 10th World Biomaterials Congress (WBC2016) 2016.05.17
Montreal,Canada
5. Nomura N, Suyalatu, Tsutsumi Y, Hanawa T, Nakamoto T, Kimura T, Kikuchi K, Kawasaki A. Eﬀect of
powder fabrication process on microstructure and mechanical properties of selective laser melted metals
for biomedical applications. 10th World Biomaterials Congress (WBC2016) 2016.05.17 Montreal,Canada― 483 ―

Life Science and Technology

Life Science and Technology

6. Chen P, Tsutsumi Y, Ashida M, Doi H, Hanawa T . Diﬀerent mechanisms of bone formation of preosteoblast on metals in vitro. 10th World Biomaterials Congress (WBC2016) 2016.05.17 Montreal,Canada
7. Tsutsumi Y, Shimabukuro M, Chen P, Ashida M, Doi H, Hanawa T. Anti-bacterial surface coating on
metallic biomaterials by simple electrochemical surface treatment. 10th World Biomaterials Congress(WBC2016) 2016.05.17 Montreal,Canada
8. Chen P, Aso T, Sasaki R, Tsutsumi T, Ashida M, Doi H, Hanawa T. Role of hierarchical topography oftitanium surface on mesenchymal stem cells adhesion and diﬀerentiation behaviours in vitro. 10th World
Biomaterials Congress (WBC2016) 2016.05.17 Montreal,Canada
9. Hanawa T, Nomura N, Ashida M, Tsutsumi Y, Doi H, Chen P. Development of Zr-Mo alloy with low
magnetic susceptibility for spinal insturuments to decrease MRI artifact. THERMEC’2016 2016.05.29
Graz,Austria
10. Hanawa T. Mesenchymal stem cell diﬀerentiation on nano pattern fabricated by femtosecond laser. International Conference on Small Science(ICSS2016) 2016.06.25 Prague, Czech Republic
11. Hanawa T. Morphological Modiﬁcation versus Chemical Modiﬁcation in Metallic Implants. 9th Paciﬁc
Rim International Conference on Advanced Materials and Processing (PRICM 9) 2016.08.01 Kyoto,Japan
12. Tsutsumi Y, Ashida M, Chen P, Doi H, Hanawa T. Evaluation of the allergenic properties of titanium
by metal ion release test in a simulated body environment. 9th Paciﬁc Rim International Conference on
Advanced Materials and Processing (PRICM 9) 2016.08.01 Kyoto,Japan
13. Hanawa T. Vriety of diferentiation of hMSC on nano-pattern fabricated by femto-second laser. 2nd Boneand Biomaterials Workshop 2016.08.07 Inari, Finland
14. Tsutsumi Y. Electrochemical surface modiﬁcation techniques for metallic biomaterials. 2nd Bone andBiomaterials Workshop 2016.08.07 Inari, Finland
15. Hanawa T. Research and development of metallic biomaterials meeting clinical demand. 9th Latin American Congress on Artiﬁcial Organs and Biomaterials (COLAOB 2016) 2016.08.24 Iguas falls, Brazil
16. Correa DRN, Rocha LA, Doi H, Tsutsumi Y, Hanawa T. Micro-arc oxidation of Ti-15Zr-based alloys forosseointegrative implants. XVSBPMat 2016.09.25 Campinas, Brazil
17. Tsukada J, Tsutsumi Y, Ashida M, Chen P, Doi H, Sugawara U, Muto I, Hara N, Hanawa T. Pitting
corrosion behavior of zirconium in a simulated body ﬂuid containing chloride ions. Paciﬁc Rim Meetingon Electrochemical and Solid-State Science (PRiME2016) 2016.10.02 Hawaii, USA
18. Shimabukuro M, Tsutsumi Y, Ashida M, Chen P, Doi H, Hanawa T. Electrochemical surface treatment
for controlling the release of silver ion as antibacterial agent on titanium. Paciﬁc Rim Meeting on Electrochemical and Solid-State Science (PRiME2016) 2016.10.02 Hawaii, USA
19. Tsutsumi Y, Ashida M, Chen P, Doi H, Hanawa T. Corrosion behaviors and metal ion releases of metallic
biomaterials in simulated body ﬂuid. Paciﬁc Rim Meeting on Electrochemical and Solid-State Science(PRiME2016) 2016.10.02 Hawaii, USA
20. Liu H, Isik M, Niinomi M, Chen P, Hanawa T, Fujii H. Microstructure and mechanical properities ofa biomedical Co-Cr-Mo alloy processed by high pressure torsion. The 1st International Symposium on
Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development(iLIM-1) 2016.10.17 Osaka, Japan
21. Uo M, Wada T, Maekawa M, Kanno Z, Ono T, Hanawa T. Development of super engineering plasticV(PEEK) made orthodontic wires. The 1st International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development (iLIM-1) 2016.10.17 Osaka, Japan
22. Miyake M, Tsukamoto M, Sato Y, Oga T, Nagai A, Chen P, Hanawa T. Dependence of Ti plate surface
morphology varied by femtosecond laser irradiation on cell spreading . The 1st International Symposiumon Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development(iLIM-1) 2016.10.17 Osaka, Japan― 484 ―

Life Science and Technology

Life Science and Technology

23. Chen P, Kawa T, Miyake M, Ooga T, Tsukamoto M, Ashida M, Tsutsumi Y, Doi H, Hanawa T. Calciﬁcation of preosteoblast on nano topography fabricated by femtosecond laser. The 1st International
Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher
Development (iLIM-1) 2016.10.17 Osaka, Japan
24. Tsutsumi Y. Surface modiﬁcation of biomedical metallic materials. The 1st International Symposiumon Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development(iLIM-1) 2016.10.17 Osaka, Japan
25. Tsutsumi Y, Shimabukuro M, Ashida M, Chen P, Doi H, Hanawa T. Simple electrochemical surface
treatment to achieve antibacterial property on titanium. The 1st International Symposium on Creationof Life Innovation Materials for Interdisciplinary and International Researcher Development (iLIM-1)
2016.10.17 Osaka, Japan
26. Morita M, Ashida M, Tsutsumi Y, Nomura N, Chen P, Doi H, Hanawa T. Development of MRI compatible
Zr-1Mo alloy. Biomaterials International 2016 2016.10.30 Kenting,Taiwan
27. Hanawa T. Biofunctionalization of metals meeting clinical demand. Biomaterials International 20162016.10.30 Kenting,Taiwan
28. Honma K, Shimojo M, Doi H, Ashida M, Chen P, Tsutsumi Y, Hanawa T. A designing and properties ofmultidimensional system Zr alloys. Biomaterials International 2016 2016.10.30 Kenting,Taiwan
29. Kajima Y, Takaichi A, Kittikundecha N, Nakamoto T, Doi H, Takahashi H, Nomura N, Hanawa T,
Wakabayashi N. Eﬀects of heat treatment on the microstructure and mechanical property of selective
laser melted Co–Cr–Mo alloys. International Dental Materials Congress 2016 (IDMC2016) 2016.11.04
Bali, Indonesia
30. Hanawa T. Surface treatment and modiﬁcation of metals to add biofunctions. International Dental Materials Congress 2016 (IDMC2016) 2016.11.04 Bali, Indonesia
31. Tsutsumi Y, Shimabukuro M, Ashida M, Chen P, Doi H, Hanawa T. The surface treatment to realizeboth antibacterial property and bioactivity. International Dental Materials Congress 2016 (IDMC2016)2016.11.04 Bali, Indonesia
32. Takaichi A, Kajima Y, Kittikundecha N, Nakamoto T, Nomura N, Tkahashi H, Hanawa T, Wakabayashi
N. Eﬀects of support structure on the fatigue strength of selective laser melted Co-Cr-Mo clsps. International Dental Materials Congress 2016 (IDMC2016) 2016.11.04 Bali, Indonesia
33. Oishi M, Tsutsumi Y, Chen P, Doi H, Hanawa T. Change in surface of yttria-stabilized zirconia in waterand Hanks’solution characterized using XPS. International Dental Materials Congress 2016 (IDMC2016)2016.11.04 Bali, Indonesia
34. Tsutsumi Y, Hanawa T. Evaluation of accelerated corrosion reaction of titanium under thin electrolyte
layer simulating oral cavity environment. The 3rd Joint Symposium between IBB/TMDU and Chulalongkorn University on Biomedical Materials and Engineering 2016.12.15 Chulalongkorn University,
Bangkok, Thailand
35. Biofunctinaolization of metallic biomaterials by plasma electrolytic oxidation technique. 26th Annual
Meeting of MRS-J (The Materials Research Society of Japan) 2016.12.19 Yokohama― 485 ―

Life Science and Technology

Life Science and Technology

Inorganic Biomaterials

Professor Kimihiro Yamashita
Associate Prof. Miho Nakamura
Assistant Prof. Naohiro Horiuchi
Research Associate Takako Takuma(1)

Outline(1) Development of Electrovector ceramics
Some ceramics, such as a hydroxyapatite, are able to be ionically polarized by thermoelectrical treatments.Consequently,
the polarized ceramics have large and time-durable induced electrostatic charges on their surfaces. The eﬀects
of the induced charges profoundly dominate the proximate few millimeter regions. We named the eﬀects Electrovector eﬀects and develop Electrovector ceramics deﬁned as ceramics emiting the Electrovector Eﬀects.
(2) Control of electrical space on Electrovector ceramic
To translate the Electrovector ceramics into practical applications for medical devises, electrical space on Electrovector ceramics should be suitably controlled under the poling process. We are evaluating the poling mechanismsof some bioceramics, based on the various disciplines. In particular, we are putting emphasis on the relationshipbetween the origin of electrical space and the crystal structure on the surface of the polarized bio-ceramics. The
crystal defect, crystal distortion and ﬁne change of ion composition of Electrovector ceramics polarized undervarious conditions are systematically investigated.
(3)Manipulation of biological responses by Electrovector ceramics
The electrostatic energies of the Electrovector eﬀects aforementioned dominate the limited proximate areas andcan control reactions locally. Therefore, the Electrovector ceramics can manipulate biological responses in atarget space by both of the surface character and the electrostatic energies of the Electrovector ceramics at ion
and tissue levels. We have demonstrated that the Electrovector ceramics enhanced protein adsorption, proliferation, adhesion, and diﬀerentiation of cultured cells on the ceramics as well as osteoconductivities in vivo bymolecular biological and immunological detections.
(4) Development of applicatable devices by ceramic technologies
We apply the Electrovector ceramics aforementioned to implant systems, such as artiﬁcial bones, bone joints,
tooth roots, and are developing implantable devices with autograft-like osteoconductivities. We are undergoing
improvements of sol-gel method for hydroxyapatite thin ﬁlm coating and materials for vascular regeneration.
We are extending our researches based on ceramic technologies farther, such as a control of oral environment,
an improvement of oral esthetics, more eﬀective and precise diagnosis systems for clinical laboratory medicine.

(2)

Publications[Original Articles]
1. Kosuke Nozaki, Risa Yamada, Hiroyuki Miura, Kimihiro Yamashita, Akiko Nagai. Antibacterial activityof silver nanoparticles coanting on zirconia against oral bacteria Visual-JW 2016. 2016; 41― 486 ―

Life Science and Technology

Life Science and Technology

2. Norio Wada, Naohiro Horiuchi, Kohei Ohta, Shintaro Urasaki, Kohei Yamauchi, and Kimihiro Yamashita.
Controlled in Vivo Nacre Formation in Flat Pearls with Hydroxyapatite Bioceramic Nuclei Crystal Growth& Design. 2016.01; 16(1); 167-173
3. Norio Wada, Naohiro Horiuchi, Kastuyuki Mukougawa, Kosuke Nozaki, Miho Nakamura, Akiko Nagai,
Toshinori Okura, and Kimihiro Yamashita. Electrostatic induction power generator using hydroxyapatite
ceramic electrets Materials Research Bulletin. 2016.02; 74; 50-56
4. Naohiro Horiuchi, Norio Wada, Kosuke Nozaki, Miho Nakamura, Akiko Nagai, and Kimihiro Yamashita.
Dielectric relaxation in monoclinic hydroxyapatite: Observation of hydroxide ion dipoles Journal of Applied Physics. 2016.02; 119(8); 084903-6[Books etc]
1. K. Yamashita. Electrically Active Materials for Medical Devices. Imperial College Press, 2016.10[Misc]
1. Kimihiro Yamashita, Naohiro Horiuchi. Cutting-edge Bio-interface Engineering for Design and Productionof Innovative Bioceramics International Journal of Metallurgical & Materials Engineering. 2016; 2(2); 128
2. T. Okura, N. Yoshida, K. Yamashita. Na+ Superionic Conducting Silicophosphate Glass-Ceramics-Review
Solid State Ionics, 285. 2016.02; 28; 143-154
3. Kazuhiro Kohata, Soichiro Itoh, Naohiro Horiuchi, Taro Yoshioka, and Kimihiro Yamashita. The roleof the collaborative functions of the composite structure of organic and inorganic constituents and theirinﬂuence on the electrical properties of human bone Bio-Medical Materials and Engineering. 2016.08;
27(2-3); 305-314[Conference Activities & Talks]
1. Iwata N, Nozaki K, Miura H, Yamashita K, Nagai A. Surface modiﬁcation of Ti implant with controlled
micro/nano-structured topography. AADR/CADR Annual Meeting & Exhibition 2016.03.17 Los Angeles,
CA, USA
2. Risa Yamada, Kosuke Nozaki, Hiroyuki Miura, Kimihiro Yamashita and Akiko Nagai. Antimicrobial activity of silver nanoparticle-coated yttria-stabilized zirconia. AADR/CADR Annual Meeting & Exhibition
2016.03.17 Los Angeles, CA, USA
3. Fujita K, Nozaki K, Yamashita K, Miura H, and Nagai A. Diﬀerentiation of periodontal ligament cell onhydroxyl- and carbonated-apatites. AADR/CADR Annual Meeting & Exhibition 2016.03.19 Los Angeles,
CA, USA4. Nozaki K, Fujita K, Miura H, Yamashita K, Nagai A. Bone remodeling-optimization of carbonated apatite
for guided bone regeneration. AADR/CADR Annual Meeting & Exhibition 2016.03.19 Los Angeles, CA,USA
5. Akiko Nagai, Takaaki Masutani, Kosuke Nozaki, Kimihiro Yamashita. Electric stimulation induced bypolarized hydroxyapatite regulates osteoblast proliferation through ERK activation. 10th World Biomaterials Congress 2016.05.18
6. Kosuke Nozaki, Kazuhisa Fujita, Naohiro Horiuchi, Miho Nakamura, Hiroyuki Miura, Kimihiro Yamashita, Akiko Nagai. In vivo evaluation of the role of carbonate ion in hydroxyapatite for bone remodeling. 10th World Biomaterials Congress 2016.05.20
7. Fabrication and evaluation of yttria-stabilized zirconia with antimicrobial properties. 2016.07.09
8. Osteoblast behavior on controlled micro/nano structured titanium surface. 2016.07.09
9. Alkaline Phosphatase Activity of Periodontal Ligament Cell on Collagen-coated Hydroxyl-Apatites. 2016.07.09
10. In vivo evaluation of calcium phosphate with optimized osteoconductivity and solubility by carbonate ion.
2016.07.10― 487 ―

Life Science and Technology

Life Science and Technology

11. Fabrication of antibacterial yttria-stabilized zirconia using silver nanoparticle . 2016.09.09
12. Naohiro Horiuchi, Kosuke Nozaki, Miho Nakamura, Akiko Nagai, Kimihiro Yamashita. Phase transitionin calcium hydroxyapatite observed by thermally stimulated current method. The 9th International Symposium on Inorganic Phosphate Materials (ISIPM-9) 2016.09.26 Tokyo Metropolitan University, Tokyo,
Japan
13. Kosuke Nozaki, Risa Yamada, Hiroyuki Miura, Kimihiro Yamashita, Akiko Nagai. Antibacterial activityof silver nanoparticles coating on zirconia against oral bacteria. The 1st International Symposium on
Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development
2016.10.17
14. Naohiro Horiuchi, Norio Wada, Kosuke Nozaki, Miho Nakamura, Akiko Nagai, and Kimihiro Yamashita.
Surface charges on hydroxyapatite electrets studied by Kelvin probe method. The 28th Annual Meeting of
the International Society for Ceramics in Medicine (Bioceramics 28) 2016.10.20 Charlotte, North Carolina,
USA[Patents]
1. METHOD FOR CONTROLLING ORGANISMS AND MATERIAL THEREFORE, METHOD FOR SELECTIVE ADSORPTION OF PROTEINS AND MATERIAL THEREFORE, CEMENT MATERIAL
AND BIOMATERIAL, Patent Number：US6777214B1
2. Material for controlling organisms and for selective adsorption of protein, cement and biomaterial, Patent
Number：EU 00104225.8-2107― 488 ―

Life
LifeScience
Science andand Technology
Technology

Organic Materials

Professor:Nobuhiko YUIAssistant Professor:Atsushi TAMURA
Assistant Professor:Yoshinori ARISAKA
Secretary:Nanae NISHI(1)

Research

1. Design of Dynamic Biomaterials Surfaces
Biomaterials surfaces with dynamic properties are designed by utilizing a molecularly movable architecture ofpolyrotaxanes, and examined their eﬀects on a variety of interactions with living body.
2. Modulation of Cellular Functions by Dynamic Ligand-Polymers
Biologically active ligands are introduced into cyclic molecules in polyrotaxanes, and examined the eﬀects of
their movability on multivalent interactions with receptor proteins and the subsequent events including intracellular metabolisms.
3. Modulation of Cellular Functions by Complexation of Intracellular Functional Supermolecules with Biomolecules
Cytocleavable polyrotaxanes to form complexes with biomolecules such as nucleic acid and protein are designed
and their cellular functions are evaluated.
4. Cytocleavable Polyrotaxanes as Molecular Therapeutics for Congenital Metabolic disorders
Cyclodextrins released from cytocleavable polyrotaxanes speciﬁcally at lysosomes are eﬀective to reduce excess
cholesterol accumulation in lysosomes as well as ameroliate impaired autophagy in lysosomal disorders.

(2)

Publications[Original Articles]
1. Yoshinori Arisaka, Jun Kobayashi, Kazuo Ohashi, Kohei Tatsumi, Kyungsook Kim, Yoshikatsu Akiyama,
Masayuki Yamato, Teruo Okano. A heparin-modiﬁed thermoresponsive surface with heparin-binding
epidermal growth factor-like growth factor for maintaining hepatic functions in vitro and harvesting hepatocyte sheets Regenerative Therapy. 2016; 3; 97-106
2. Yoshinori Arisaka, Yuka Nishijima, Shin-ichi Yusa, Naoya Takeda. Photo-induced in situ crosslinking ofpolymer brushes with dimethyl maleimide moieties for dynamically stimulating stem cell diﬀerentiation
Journal of Biomaterials Science: Polymer Edition. 2016; 27(13); 1331-1340
3. Ai Ozaki, Yoshinori Arisaka, Naoya Takeda. Self-driven perfusion culture system using a paper-based
double-layered scaﬀold Biofabrication. 2016; 8(3); 035010
4. Atsushi Tamura, Asato Tonegawa, Yoshinori Arisaka, Nobuhiko Yui. Versatile synthesis of end-reactive
polyrotaxanes applicable to fabrication of supramolecular biomaterials Beilstein Journal of Organic Chemistry. 2016; 12; 2883-2892― 489 ―

Life Science
Science andand Technology
TechnologyLife

5. A. Tamura, K. Nishida, N. Yui. Lysosomal pH-inducible supramolecular dissociation of polyrotaxanes
possessing acid-labile N-triphenylmethyl end groups and their therapeutic potential for Niemann-Picktype C disease. Science and Technology of Advanced Materials . 2016.07; 17(1); 361-371
6. K. Nishida, A. Tamura, N. Yui. Tailoring the temperature-induced phase transition and coacervate
formation of methylated β-cyclodextrins-threaded polyrotaxanes in aqueous solution. Macromolecules.
2016.08; 49(16); 6021-6030
7. M. Terauchi, T. Inada, A. Tonegawa, A. Tamura,S. Yamaguchi, K. Harada, N. Yui. Supramolecularinclusion complexation of simvastatin with methylated β-cyclodextrins for promoting osteogenic diﬀerentiation. International Journal of Biological Macromolecules. 2016.11; 93; 1492-1498[Conference Activities & Talks]
1. Yoshinori Arisaka, Atsushi Tamura, Nobuhiko Yui. Supermolecule-coated metal surfaces for fabricating
dynamic biointerfaces. The 1st International Symposium on Creation of Life Innovation Materials forInterdisciplinary and International Researcher Development 2016.10.17 Osaka, Japan
2. Atsushi Tamura. Development of acid-labile polyrotaxanes for the treatment of Niemann-Pick type C
disease. The 8th International Workshop on Advanced Materials Science and Nanotechnology (IWAMSN
2016) 2016.11.09 Ha Long City, Vietnam
3. Yoshinori Arisaka, Atsushi Tamura, Nobuhiko Yui. Polyrotaxane-based 2D and 3D materials design forbiomedical applications. International Symposium on Biomedical Engineering 2016.11.10 Tokyo, Japan
4. Yoshinori Arisaka, Atsushi Tamura, Nobuhiko Yui. Supramolecular surfaces with hydrated molecular mobility for constructing dynamic biointerfaces. 3rd International Conference on Biomaterials Science(ICBS2016) 2016.11.28 Tokyo, Japan― 490 ―

Life
LifeScience
Science andand Technology
Technology

Medicinal Chemistry

Professor Hirokazu TAMAMURA, Ph.D.
Associate Professor Wataru NOMURA, Ph.D.
Assistant Professor Takaaki MIZUGUCHI,Ph.D.
Assistant Professor Nami OHASHI,Ph.D.
Technical Assistant Miho TANABE,Ami MASUDA
Assistant Tomoe KAMEI
Adjunct Lecturer Takaki KOIDE
Graduate students
D3 Shohei TAKETOMI,Takuya KOBAYAKAWA,Hikaru TAKANO,Takayoshi HIGASHI
D2 Kiju KONNO,Kei TOYAMA,Kenichi HIRAMATSU,Yuzuna HONDA,Maxwell SAKYIAMAH
D1 Daisuke MATSUMOTO,Daisuke MIYAKI
M2 Yusuke ISHIDA,Moemi KANEKO,Naoyuki KURATA,Ayumi SAITOU,Toshihiko TSUKINOKI
M1 Kento EBIHARA,Tsukasa HASHIMOTO
External Collaborators
Yuko YAMADA,Keisuke HARADA(1)

Outlinefor regulation of
Research in the lab is mainly focused to two topics; 1) development of artiﬁcial enzymes
gene functions and (2) exploration and analyses of cellular functions by methods based on peptide chemistry.
Students will learn how to design research, experimental techniques, and analysis methods of research data.
Research themes are related to multiple research ﬁelds such as molecular biology, chemistry, chemical biology,
and synthetic biology.

(2)

Research

1.Development of conformational-constrained templates for drug discovery.
Drug-discovery templates for conformational restriction, which enable pharmacophores of bioactive compounds(ex. peptides) to be suitably disposed in three-dimensional space, are being developed. Drug discovery for thechemotherapy of cancer, AIDS, Alzheimer s disease, rheumatoid arthritis, SARS, etc. is being performed basedon targeting several receptors, enzymes, etc.
2.Development of bio-probes, bio-sensing, medicinal chemistry towards chemical biology.
Bio-probes that speciﬁcally recognize each receptor or enzyme are being developed for research on chemical
biology involving imaging and sensing.
3.Structural analysis of the interactions between receptors/enzymes and their ligands.
Using X-ray crystal structural analysis, the mechanism of signal transduction operated by binding of ligands toreceptors/enzymes is being analyzed.
4. Development of applications of zinc ﬁnger protein for gene therapy and nano technology.
Utilizing DNA sequence-speciﬁc recognition of zinc ﬁnger protein, technologies for DNA recombination, modiﬁcations, and DNA labeling are being developed.

― 491 ―

Life Science
Science andand Technology
Technology
Life

(3)

Education

Practice
Goals/Outline:
Presenter reports about the recent topics related to molecular biomedicine from Journals. The report must comewith the backgrounds and motivations of research ﬁelds. Research designs, experimental methods, data analyses,
and perspectives for future development will be discussed about the topics. Students are also encouraged toattend to lectures for the graduate course and discuss about the topics with lecturers.
Available programs:
Lectures for the graduate course: as occasion
Journal Club: Every Thursday from 15:00 to 16:30
Lab
Goals/Outline:
Research in the lab is mainly focused to two topics; 1) development of artiﬁcial enzymes for regulation ofgene functions and (2) exploration and analyses of cellular functions by methods based on peptide chemistry.
Students will learn how to design research, experimental techniques, and analysis methods of research data.
Research themes are related to multiple research ﬁelds such as molecular biology, chemistry, chemical biology,
and synthetic biology.
Available program:Lab meeting (progress report): every week, about 1 hour per person (will be announced)(4)

Lectures & Courses

Format:Small group
Venue:
Practice: Seminar room (603) at Institute of Biomaterials and Bioengineering
Lab: Laboratory of Medicinal Chemistry (602) at Institute of Biomaterials and Bioengineering
Grading:
Practice: Attendance and report
Lab: Progress of research and report(5)

Publications[Original Articles]
1. Mizuguchi T, Harada S, Miura T, Ohashi N, Narumi T, Mori H, Irahara Y, Yamada Y, Nomura W,
Matsushita S, Yoshimura K, Tamamura H. A Minimally Cytotoxic CD4 Mimic as an HIV Entry Inhibitor.
Bioorg. Med. Chem. Lett . 2016; 2(26); 397-400
2. Ohashi N, Harada S, Mizuguchi T, Irahara Y, Yamada Y, Kotani M, Nomura W, Matsushita S, Yoshimura
K, Tamamura H. Small Molecular CD4 Mimics Containing Mono-cyclohexyl Moieties as HIV Entry Inhibitors. ChemMedChem a joint special issue with ChemBioChem: Protein–Protein Interactions. 2016;
11(8); 940-946
3. Toyama K, Mizuguchi T, Nomura W, Tamamura H. Functional Evaluation of Fluorescein-Labeled Derivatives of a Peptide Inhibitor of the EGF Receptor Dimerization Bioorg. Med. Chem. 2016; 24(16);3406-3412
4. Hiramatsu K, Honjo T, Rauniyar V, and Toste F.D. Enantioselective Synthesis of Fluoro − Dihydroquinazolones and − Benzooxazinones by Fluorination-Initiated Asymmetric Cyclization Reactions. ACS Catal.
2016; 6(1); 151-154― 492 ―

Life
LifeScience
Science andand Technology
Technology

5. Zhou L, Holt M. T, Ohashi N, Zhao A, Müller M. M, Wang B, and Muir T. W. Evidence that ubiquitylated
H2B corrals hDot1L on the nucleosomal surface to induce H3K79 methylation. Nat Commun. 2016;
6. Abduelmula A, Huang R, Pu Q, Tamamura H, Puopolo G. M & Saberi B. B. SDF-1 Controls the Muscle
and Blood Vessel Formation of the Somite. Int. J. Dev. Biol. 2016; 60(1-2-3)(29-38);
7. Kobayakawa T, Tamamura H. Eﬃcient Synthesis of Xaa-Gly Type (Z)-Chloroalkene Dipeptide Isosteres
via Organocuprate Mediated Reduction. Tetrahedron. 2016; 72(32); 4968-4971
8. Mizuguchi T, Ohashi N, Matsumoto D, Hashimoto C, Nomura W, Yamamoto N, Murakami T, Tamamura
H. Development of Anti-HIV Peptides Based on a Viral Capsid Protein. Biopolymers: Peptide Science.
2016; in press;
9. Hiramatsu K, Tamamura H. A Mild Method for the Direct Fluorination of Pyrroles by Using a Lipophilic
Anionic Phase-Transfer Catalyst. Eur. J. Org. Chem. 2016; 21; 3491-3494
10. Tanaka T, Aoki T, Nomura W, Tamamura H. Bivalent 14-mer Peptide Ligands of CXCR4 with Polyproline
Linkers with Anti-Chemotactic Activity against Jurkat Cells J. Pept. Sci. 2016; in press;
11. Nakamura T, Campbell J.R, Moore A.R, Otsu S, Aikawa H, Tamamura H, Mitsuya H. Development
and Validation of a Cell-based Assay System to Assess Human Immunodeﬁciency Virus Type 1 Integrase
Multimerization J. Virol. Methods. 2016; 236; 196-206
12. Takano H, Narumi T, Nomura W, Tamamura H. Microwave-Assisted Synthesis of Azacoumarin Fluorophores and the Fluorescence Characterization J. Org. Chem. 2016; in press;[Misc]
1. Nomura W, Mizuguchi T & Tamamura H. Multimerized HIV-gp41-derived Peptides as Fusion Inhibitors
and Vaccines Biopolymers: Peptide Science. 2016; 106(4); 622-628[Conference Activities & Talks]
1. Takano H, Narumi T, Nomura W, Furuta T, Tamamura H. Development of water-soluble and highly photosensitive 8-azacoumarin-4-ylmethyl-type photolabile-protecting groups for dynamic analysis of bioactive
molecules. 251st American Chemical Society National Meeting 2016.03.16 San Diego, USA
2. Nomura W, Ohashi N, Tamamura H. In-cell ﬂuorogenic tag-probe system for protein localization anddynamics imaging. 251st American Chemical Society National Meeting 2016.03.17 San Diego, USA
3. Nomura W, Ohashi N, Tamamura H. Tag-probe Systems for Protein Dynamics Imaging. The 16th Akabori
Conference: Japanese-German Symposium on Peptide Science 2016.05.24 Kobe, Japan
4. Nomura W, Sugii T, Tamamura H. Chemically-inducible gene regulation system utilizing sequence-speciﬁcityof TALE and dCas9. FASEB SRC: Genome Engineering: Cutting-Edge Research and Applications
2016.06.08 Lisbon、Portugal
5. Tamamura H. Mid-Size Drugs: HIV Fusion Inhibitors Based on the C34 Dimer/Trimer Derived from gp41.
The 20th Korean Peptide Protein Society Symposium 2016.06.24 Yangyang, Korea
6. Toyama K, Mizuguchi T, Nomura W, and Tamamura H. Development of New Fluorescein-Labeled Peptides with Inhibitory Eﬀect on the EGF Receptor Activation. The 20th Korean Peptide Protein Society
Symposium 2016.06.24 Yangyang, Korea
7. Nomura W, Ohashi N, Tamamura H. Protein Dynamics Imaging Using Tag-probe Systems. The 14th Chinese International Peptide Symposium & the 5th Asia-Paciﬁc International Peptide Symposium 2016.07.06
Nanjing, China
8. Miyaki D, Mizuguchi T, Nomura T, Takahashi D, Tamamura H. Synthesis of Anti-Integrase Stapled
Peptides with a Soluble Linker. The 14th Chinese International Peptide Symposium & the 5th AsiaPaciﬁc International Peptide Symposium 2016.07.06 Nanjing, China
9. Kobayakawa T, Tamamura H. Development of synthetic methodology of chloroalkene-type dipeptide
isosteres for peptidomimetics. 252nd American Chemical Society National Meeting 2016.08.23 Philadelphia, USA― 493 ―

Life Science
Science andand Technology
Technology
Life

10. Tamamura H. Mid-Size Drugs: Dimer& Trimer Mimics of HIV-gp41 Peptides as Fusion Inhibitors. 34th
European Peptide Symposium 2016 & 8th International Peptide Symposium 2016.09.05 Leipzig, Germany
11. Takano H, Nomura W, Tamamura H. Development of a Hydrophilic Caged PKC Ligand with an 8Azacoumarin Photolabile Protecting Group for the Precise Photocontrol of PKC Activation. 34th European Peptide Symposium 2016 & 8th International Peptide Symposium 2016.09.08 Leipzig, Germany
12. Toyama K, Mizuguchi T, Nomura W, Tamamura H. Functional Evaluation of Fluorescein-Labeled Peptides
Based on the EGF Receptor Dimerization Arm Sequences. 34th European Peptide Symposium 2016 &8th International Peptide Symposium 2016.09.08 Leipzig, Germany
13. Tamamura H. Mid-sized drugs based on peptide mimetics. 15th Surugadai International Symposium2016.11.29 Tokyo, Japan
14. Kobayakawa T, Matsuzaki Y, Nomura W, Hozumi K, Nomizu M and Tamamura H. Development of
Chloroalkene Dipeptide Isosteres as Peptidomimetics and Their Application. 2016.12.08 Singapore, Singapore
15. Tamamura H. Mid-size Drugs: Peptide-lead Anti-HIV Agents. The 2nd Peptides and Proteins Symposium
Singapore 2016.12.09 Singapore, Singapore
16. Nomura W, Tamamura H. Design of synthetic peptides mimicking dynamic supramolecular mechanisms in
HIV fusion as antigens and inhibitors against trimer-form of gp41. Antibody Engineering & Therapeutics
2016.12.12 San Diego, California
17. Tamamura H. Medicinal Chemistry/Peptide & Protein Chemistry/Chemical Biology- Peptidomimeticbased Mid-Size Drugs –. The 3rd Joint Symposium between Chulalongkorn University and IBB/TMDU
on Biomedical Materials and Engineering, Chulalongkorn University 2016.12.15 Bangkok, Thailand― 494 ―

Life Science and Technology

NCC Cancer Science

Visiting Professor

Hirofumi ARAKAWA

Visiting Professor

Kenkichi MASUTOMI

Visiting Professor

Ryuji HAMAMOTO

Visiting Associate Professor

Masahiro YASUNAGA

Visiting Associate Professor

Genichiro ISHII

Visiting Associate Professor

Tsutomu OHTA

Visiting Associate Professor

Masato ENARI

Visiting Lecturer

Tohru KIYONO

Visiting Lecturer

Kazunori AOKIVisiting Lecturer

Koji OKAMOTO

Visiting Lecturer

Issay KITABAYASHI

Graduate Students

D3

Kasumi OTSUBO

D2

Yuki YAMAMOTO

M2

Masaki NAGASATO,
Minori YUMOTO,
Sayaka SUGITA,
Manami MIURA

M1Marina HENMI
Hidenobu SUZUKI
Taichi IIJIMA(1)

Research

1. Carcinogenesis and molecular mechanism
2. Functions of cancer-associated genes and their alterations
3. Genomic, epigenomic and proteomic analysis of cancer and personalized medicine
4. Tumor microenvironment
5. Cancer stem cells/non-coding RNA/signaling pathway
6. Molecular target/drug delivery/diagnosis and therapy(2)

Education

To learn knowledge and skill for cancer research, students attend lectures and seminars,
and attend and/or practice research meeting, journal club, scientific meeting, etc. These
practices will enable students to develop an ability to conduct their studies as an― 495 ―

Life Science and Technology

independent cancer researcher in the future. To obtain good skills to carry out
experiments that are required for cancer research, students belong to one of ourresearch groups, and conduct their own studies under the guidance of the instructor
and/or staff. Students perform various experiments involved in genetics, genetechnology, biochemistry, cellular biology, molecular biology, physiology, experimental
animal, pathology, genomic/epigenomic/proteomic analysis, imaging, next generation
sequencing, etc.

(3)

Publications[Original Articles]
1．Kamino H, Nakamura Y, Tsuneki M, Sano H, Miyamoto Y, Kitamura N, Futamura M,
Taniguchi H, Kanai Y, Shida D, Kanemitsu Y, Moriya Y, Yoshida K, Arakawa H.
Mieap-regulated mitochondrial quality control is frequently inactivated in human colorectal
cancer. Oncogenesis. 5: e181, 2016.
2．Maida Y, Yasukawa M, Masutomi K．De novo RNA synthesis by RNA-dependent RNA
polymerase activity of TERT．Mol Cell Biol. 36: 1248-1259, 2016.
3. Piao L, Nakakido M, Suzuki T, Dohmae N, Nakamura Y, Hamamoto R. Automethylation of
SUV39H2, an oncogenic histone lysine methyltransferase, regulates its binding affinity to
substrate proteins. Oncotarget. 7: 22846-22856, 2016.
4. Saloura V, Vougiouklakis T, Zewde M, Kiyotani K, Park JH, Gao G, Karrison T, Lingen M,
Nakamura Y, Hamamoto R. WHSC1L1 drives cell cycle progression through transcriptional
regulation of CDC6 and CDK2 in squamous cell carcinoma of the head and neck. Oncotarget. 7:42527-42538, 2016.
5．Fujiwara Y, Furuta M, Manabe S, Koga Y, Yasunaga M, Matsumura Y. Imaging mass
spectrometry for the precise design of antibody-drug conjugates. Sci Rep. 6: 24954, 2016.
6．Obonai T, Fuchigami H, Furuya F, Kozuka N, Yasunaga M, Matsumura Y. Tumour imaging
by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment. Sci Rep. 6:
23613, 2016.
7．Neri S, Hashimoto H, Kii H, Watanabe H, Masutomi K, Kuwata T, Date H, Tsuboi M, Goto K,
Ochiai A, Ishii G. Cancer cell invasion driven by extracellular matrix remodeling is dependent
on the properties of cancer-associated fibroblasts. J Cancer Res Clin Oncol. 142: 437-446, 2016.
8．Saruwatari K, Ikemura S, Sekihara K, Kuwata T, Fujii S, Umemura S, Kirita K, Matsumoto S,
Yoh K, Niho S, Ohmatsu H, Ochiai A, Kohrogi H, Tsuboi M, Goto K, Ishii G. Aggressive tumor― 496 ―

Life Science and Technology

microenvironment of solid predominant lung adenocarcinoma subtype harboring with epidermal
growth factor receptor mutations. Lung Cancer. 91: 7-14, 2016.
9．Suda Y, Neri S, Hashimoto H, Higuchi Y, Ishibashi M, Sugano M, Masutomi K, Tsuboi M,
Ochiai A, Ishii G. Clonal heterogeneity in osteogenic potential of lung cancer-associatedfibroblasts: promotional effect of osteogenic progenitor cells on cancer cell migration. J Cancer
Res Clin Oncol. 142: 1487-1498, 2016.
10．Uesato S, Matsuura Y, Matsue S, Sumiyoshi T, Hirata Y, Takemoto S, Kawaratani Y, Yamai
Y, Ishida K, Sasaki T, Enari M. Discovery of new low-molecular-weight p53-Mdmx disruptors
and their anti-cancer activities. Bioorg Med Chem. 24: 1919-1926, 2016.
11. Shima Y, Yumoto M, Katsumoto T, Kitabayashi I. MLL is essential for
NUP98-HOXA9-induced leukemia. Leukemia, in press. doi: 10.1038/leu.2017.62. [Epub aheadof print][Review Articles]
1. Hamamoto R, Nakamura Y. Dysregulation of protein methyltransferases in human cancer: an
emerging target class for anticancer therapy. Cancer Sci. 107: 377-384, 2016.
2. Ishii G, Ochiai A, Neri S. Phenotypic and functional heterogeneity of cancer-associatedfibroblast within the tumormicroenvironment. Adv Drug Deliv Rev. 99: 186-1896, 2016.

[Conference Activities & Talks]

1. Yoichiro Yoshioka, Yusuke Nakamura, Ryuji Hamamoto. Critical role of SMYD3-mediatedmethylation in the oncogenic activity of AKT1. AACR/JCA 10th Joint Conference (Maui, USA),
February, 2016.
2. Xiaolan Deng, Yusuke Nakamura, Ryuji Hamamoto. PRMTI enhances formation ofchromosomal passenger complex (CPC) through arginine methylation of INCENP in humancancer cells. AACR/JCA 10th Joint Conference (Maui, USA), February, 2016.
3. Theodore Vougiouklakis, Vassiliki Saloura, Yusuke Nakamura, Ryuji Hamamoto.
SUV420H1 enhances the phosphorylation and transcription of ERK1 in cancer cells. AACR
107th annual meeting (New Orleans, USA), April, 2016.
4. Xiaolan Deng, Yusuke Nakamura, Ryuji Hamamoto. SMYD2-mediated lysine
methylation regulates nuclear transport of beta-catenin in human cancer cells.

― 497 ―AACR

Life Science and Technology

107th annual meeting (New Orleans, USA), April, 2016.
5．Masahiro Yasunaga, Yasuhiro Matsumura. The role of the taurine transporter SLC6A6 inpromoting prosurvival activity and multidrug resistance of colorectal cancer.
American Association for Cancer Research Annual Meeting (New Orleans, USA), April, 2016.
6. Masahiro Yasunaga, Shino Manabe, Atushi Tsuji,

Masaru Furuta, Koretsugu Ogata, Yuki

Fujiwara, Yoshikazu Koga, Yasuhiro Matsumura. Development of CAST therapy by utilizing
molecular imaging. World Molecular Imaging Congress (New York, USA), September, 2016.
7. Masahiro Yasunaga, Shino Manabe, Atushi Tsuji,

Masaru Furuta, Koretsugu Ogata, Yuki

Fujiwara, Yoshikazu Koga, Yasuhiro Matsumura. Development of ADC utilizing molecularimaging. 27th Antibody Engineering & Therapeutics (San Diego, USA), December, 2016.
8. Shima Y, Yumoto M, Katsumoto T, Kitabayashi I. MLL Is Essential for
NUP98-HOXA9-Induced Leukemia．The 10th AACR-JCA Joint Conference (Maui, USA),
February, 2016.

― 498 ―

Life
LifeScience
Science andand Technology
Technology

Cellular and Molecular Medicine

associate professor Yumiko Oishi MD., Ph.D
assistant professor Shinichiro Hayashi Ph.D
assistant professor Sumio Hayakawa Ph.D
(1)

OutlineCardiovascular disease, as a consequent of the obesity related metabolic syndrome, remains a signiﬁcant causeof morbidity and mortality in industrialized societies. A major eﬀort of our laboratory has been to investigate the molecular mechanism of an initiation and progression of metabolic syndrome from the viewpoint oftranscriptional regulation. Since macrophages contribute to all phages of the pathogenesis of atherosclerosis,
we have extensively studied the macrophage diversity that respond to various stress within tissue environment.
Additionally, it is recognized that sarcopenia (skeletal muscle loss with) is important for the pathogenesis of
metabolic syndrome. The long term goals of our current study are to elucidate: 1) the mechanism of the link
between cellular metabolism and immune response of macrophage 2) the mechanism of chronic inﬂammationthat leads to metabolic syndrome, and 3) the mechanism responsible for pathogenesis of sarcopenia and skeletal
muscle degeneration.
(2)

Research

1．Mechanisms of Coordinated regulation of inﬂammatory response and lipid homeostasis in macrophage
Chronic low-grade inﬂammation has been recognized as a key contributing factor in the onset and progressionof metabolic syndrome and atherosclerosis. As a multifunctional eﬀector cell, macrophage play pivotal roles inboth the enhancement and resolution of this inﬂammatory process. By utilizing molecular biology technique,lipidomics and bioinformatics, we found that the lipid homeostasis is coordinately regulated with inﬂammatory
response in macrophage. TLR4 activation rapidly, and transiently inhibits Liver X receptor (LXR) signaling, andsubsequently activates Sterol regulatory element-binding protein (SREBP). In the late phase of inﬂammation,
LXR and SREBP work together to increase anti-inﬂammatory fatty acid synthesis, necessary for a resolutionof inﬂammation. Thus, transcriptional/signaling network involving LXR and SREBP play a pivotal role in
the regulation of lipid homeostasis and cellular function. By elucidating the crosstalk between cellar function
and metabolism, we would be able to accumulate beneﬁcial knowledge to develop novel therapeutic strategy
targeting macrophages for the prevention and treatment of metabolic syndrome.
2. Mechanism of skeletal muscle degeneration
Skeletal muscle consume 40% of total energy, playing a key role for the pathogenesis of metabolic syndrome.
Sarcopenia is the degenerative loss of skeletal muscle mass, quality and strength associated with aging. Although
the causes and mechanisms of sarcopenia still remains unclear, one of the hypotheses is reduction of the numberof satellite cells, stem cells in adult muscle, and failure of satellite cell activation. We identiﬁed KLF5 as a novel
factor that play a pivotal role in skeletal muscle degeneration. KlF5 is a Zinc-ﬁnger transcription factor involvedin the self-renewal and proliferation of embryonic stem cell and cancer stem cell. KLF5 is transiently inducedin the myoblast during diﬀerentiation and it plays critical role for muscle degeneration and repair. Although
Klf5 is not expressed in the quiescent satellite cells, its expression is dramatically increased in the satellite cellswith age. Now we are testing the hypothesis whether the dysregulation of Klf5 causes a mulfunction of satellite
cells.

― 499 ―

Life Science
Science andand Technology
Technology
Life

(3)

Education

Lecture: Doctoral Program, Life Science and Technology Track #6110, Bioscience I
Lecture: Master’s Program, Medical and Dental Science and Technology Track, Molecular biology
Lecture: School of Dentistry, Second grade, Basic molecular mechanisms of life

(4)

Publications[Original Articles]
1. Rumi Hachiya, Takuya Shiihashi, Ibuki Shirakawa, Yorihiro Iwasaki, Yoshihiro Matsumura, Yumiko Oishi,
Yukiteru Nakayama, Yoshihiro Miyamoto, Ichiro Manabe, Kozue Ochi, Miyako Tanaka, Nobuhito Goda,
Juro Sakai, Takayoshi Suganami, Yoshihiro Ogawa. The H3K9 methyltransferase Setdb1 regulates TLR4mediated inﬂammatory responses in macrophages. Sci Rep. 2016.06; 6; 28845
2. Taisho Yamada, Hiromasa Horimoto, Takeshi Kameyama, Sumio Hayakawa, Hiroaki Yamato, Masayoshi
Dazai, Ayato Takada, Hiroshi Kida, Debbie Bott, Angela C Zhou, David Hutin, Tania H Watts, Masahiro
Asaka, Jason Matthews, Akinori Takaoka. Constitutive aryl hydrocarbon receptor signaling constrains
type I interferon-mediated antiviral innate defense. Nat. Immunol.. 2016.06; 17(6); 687-694
3. Shinichiro Hayashi, Ichiro Manabe, Yumi Suzuki, Frédéric Relaix, Yumiko Oishi. Klf5 regulates muscle diﬀerentiation by directly targeting muscle-speciﬁc genes in cooperation with MyoD in mice. Elife.
2016.10; 5;[Books etc]
1. Takuji Suzuki, Noriyuki Miyoshi, Sumio Hayakawa, Shinjiro Imai, Mamoru Isemura, and Yoriyuki Nakamura. Beverage Impacts on Health and Nutrition. Springer International Publishing, 2016
[Misc]
1. Sumio Hayakawa, Kieko Saito, Noriyuki Miyoshi, Tomokazu Ohishi, Yumiko Oishi, Mamoru Miyoshi,
Yoriyuki Nakamura. Anti-Cancer Eﬀects of Green Tea by Either Anti- or Pro- Oxidative Mechanisms.
Asian Pac. J. Cancer Prev.. 2016; 17(4); 1649-1654
2. Yumiko Oishi, Ichiro Manabe. Chronic inﬂammation and atherosclerosis 2016.01; 45(1); 12-17
3. Yumiko Oishi, Ichiro Manabe. Integrated regulation of the cellular metabolism and function of immune
cells in adipose tissue. Clin. Exp. Pharmacol. Physiol.. 2016.03; 43(3); 294-303
4. Yumiko Oishi, Ichiro Manabe. Immune system in the organ crosstalk and metabolism Journal of Clinicaland Experimental Medicine. 2016.05; 257(6); 597-600
5. Yumiko Oishi, Ichiro Manabe. Transcriptional, epigenetic, and transcriptome analysis of macrophage
Journal of Clinical and Experimental Medicine. 2016.06; 257(6); 733-737
6. Yumiko Oishi, Ichiro Manabe. Macrophages in age-related chronic inﬂammatory diseases Aging and
Mechanisms of Disease. 2016.07; 2; 16018
7. Yumiko Oishi, Ichiro Manabe. Zinc-ﬁnger transcription factors 2016.08; 175-180
8. Yumiko Oishi, Ichiro Manabe. parabiosis in aging science Anti-Aging medicine. 2016.10; 12(5); 650-656
9. Yumiko Oishi, Ichiro Manabe. Macrophage lineages in atherosclerosis Journal of Clinical and Experimental
Medicine. 2016.10; 259(5); 475-479
10. Yumiko Oishi, Ichiro Manabe. B cells in adipose tissue inﬂammation The Medical Frontline. 2016.11;
71(917); 2217-2221― 500 ―

Life
LifeScience
Science andand Technology
Technology[Conference Activities & Talks]
1. Yumiko Oishi. Coordinated regulation of immune response, circadian rhythm and cellular metabolism inmacrophage as a novel therapeutic target. The 80th Annual Scientiﬁc meeting of the Japanese Circulation
Society 2016.03.20
2. Sumio Hayakawa, Yumiko Oishi . The crosstalk between chronic inﬂammation and lipid metabolism. The
24th International Symposium on Molecular Cell Biology of Macrophage 2016.06.04 Tokyo, Japan
3. Yumiko Oishi. Coordinated regulation of inﬂammatory response and lipid metabolism in macrophage.
The 48th annual scientiﬁc meeting of the Japan Atherosclerosis society 2016.07.15 Tokyo
4. Shinichiro Hayashi, Ichiro Manabe, Yumi Suzuki, Yumiko Oishi. Klf5 regulates skeletal muscle diﬀerentiation by directly targeting muscle-speciﬁc genes. FASEB SRC -KLF AND SP TRANSCRIPTION
FACTORS IN DISEASE AND REGENERATIVE MEDICINE- 2016.08.07 Snowmass, CO
5. S. Hayakawa, Y. Oishi . Inﬂammatory response regulates the transcription factor SREBP activation.
. The 57th International Conference on the Bioscience of Lipids (ICBL) 2016.09.04 Chamonix-MontBlanc,France― 501 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology

Lifetime Oral Health Care Sciences

Professor Shinichi ARAKAWA
Junior Associate Professor Keiko KONDO
Assistant Professor Masayo YASUDA
Graduate Student Mizuki DOINO,Tona WATANABE
Resident Hidehiro SHIOYAMA(1)OutlineMain objective of Lifetime Oral Health Care Sciences is to understand and learn how oral health care contributes
to the preservation of general health and healthy life expectancy. Students also learn the newest knowledge on
oralpathology and oral health promotion, and are trained to master the modality of oral health care.
Regarding research, the eﬀects of the functional waters to organism and clinical application of them were
investigated.

(2)

Research

1）Clinical and basic studies on Ozone nano-bubble water（NBW3）:antimicrobila activity and eﬀects to eukaryotic cells (induction of anti-oxydant capacities and wound healing activities etc.)
2）Study on virulence factors of periodontopathic bacteria3）Development of education system for dental（oral）hygienists to prevent oral diseases4）Development of assessment program in technical education for dental（oral）hygienists(3)

Education

Main objective of Lifetime Oral Health Care Sciences is to understand and learn how oral health care contributes
to the preservation of general health and healthy life expectancy. Students also learn the newest knowledge on
oralpathology and oral health promotion, and are trained to master the modality of oral health care.

(4)

Lectures & Courses

Main objective of Lifetime Oral Health Care Sciences is to understand and learn how oral health care contributes
to the preservation of general health and healthy life expectancy. Students also learn the newest knowledge on
oralpathology and oral health promotion, and are trained to master the modality of oral health care

(5)

Clinical Services & Other Works

Oral care clinic provides prevention of oral diseases, such as dental caries or periodontal diseases for maintaining
patients oral and general health in a lifetime.

― 502 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology(6)

Clinical Performances

Oral care clinic provides prevention of oral diseases, such as dental caries or periodontal diseases for maintaining
patients oral and general health in a lifetime.
(7)

Publications[Original Articles]
1. H. Otuska, S. Arakawa, A. Kinoshita, K. Shinada, et al.. Development of an interprofessional education
program where dental hygiene students instruct medical and dental students. Journal of Dental Education.
2016; 80; 1062-1070
2. Doino, Mizuki; Arakawa, Shinichi; Yokoyama, Miki; Sasaki, Yoshiyuki; Kondo, Keiko; Yasuda, Masayo..
Evaluation of the relationship between salivary concentration of anti-heat shock protein immunoglobulin
and clinical manifestations of Beh?et’s disease. Scandinavian Journal of Rheumatology. 2016;[Misc]
1. Shinichi Arakawa. Ozone antiseptic shows potential for treating severe gum infections Asia Research News2015.
[Conference Activities & Talks]
1. Atsushi Oishi, Hitoyata Shimokawa, Wit Yee Wint, Shinichi Arakawa, Michiyo Miyashin. Eﬀects ofNanobubble water on human cells derived from impacted supernumerary teeth. 10th Biennial Conferenceof the Pediatric Dentistry Association of Asia in conjunction with 54th Annual Conference of the JapaneseSociety of Pediatric Dentistry 2016.05.26 Tokyo
2. Jun Tsuruta, Kanako Noritake, Kouji Mizutani, Naoko Seki, Keiko Kondou, Shinichi Arakawa, Kouji
ARAKI. A new course Working in a team . SEAADE Annual Scientiﬁc Meeting 2016.09.08 Ho Chi Minhcity, Vietnam
3. Jun Tsuruta,Kanako Noritake, Koji Mizutani, Naoko Seki,Keiko Kondo, Shinichi Arakawa,Kouji Araki.
Implementing a new IPW course in the dental student clinic. Peking University School of Stomatology·
Tri-university 2016.10.27 Peking University School of Stomatology
4. Seki N, Moross J, Otsuka H, Sunaga M, Naito M, Takatsuna Y, Kondo K, Shinada K, Morio I, Kinoshita
A. Learning outcome provided by ESL clinical simulation teaching materials for dental hygiene education.
81st Annual Meeting of the Stomatological Society 2016.11.26 Tokyo
5. Watanabe T. ,Yasuda M,. Kondo K., Arakawa S.,. Evaluation for the method of intervension for oaral
health improvement for people with disabilities at the day care center.. The Stomatological Society,Japan
2016.11.26 Tokyo Medical and Dental University― 503 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology

Oral Care for Systemic Health Support

Professor KABASAWA Yuhji(1)

Outline(1) Education
We teach the knowledge and skills necessary for oral health activities through classes in charge. Speciﬁcally,
we will teach about the relationship between oral health and general health such as perioperative oral function
management and periodontal disease and diabetes. In addition to teaching health assessment of the oral
and maxillofacial area, we will acquire basic knowledge and skills through vital signs measurement practice,
emergency life-saving activity practice, which is one of general health evaluation indicators.
Furthermore, we lecture on the pathology, pathology, diagnosis and treatment related to mandibular oral cavity
disease, and teach necessary knowledge on oral health education, prevention of oral diseases of people with basic
diseases in the medical ﬁeld.
(2) Research
We will conduct research to support maintenance and promotion of health by oral health. Especially contributeto people’s health and well-being through research on perioperative oral cavity function management, researchon oral care of people with underlying diseases, research on regeneration of jawbone with FGF-2, etc.
(3) ClinicalIn order to maintain and promote general health through oral health, we cooperate with each outpatient at thedentistry hospital and do dental prophylactic treatment of the patient and oral health education at oral care
outpatient. In addition to oral care for inpatients at dental and medical hospital while working in cooperation
with nurses, nutritionists, pharmacists and others, they practice oral care according to the condition of patientsas a member of team medicine.
(2)

Research

1. Oral health care of patients with oral cancer, cleft lip and palate and other oral diseases
2. Oral health related QOL of patients with oral cancer, cleft lip and palate, dry mouth
3. Basic research of tooth brush, peeling sponge and tooth paste
4. Morphological, functional research, and oral health of patients with cleft lip and palate
5. Research for safety in supplements in oral functions
6. Basic research for bone regeneration using FGF-2.
(3)

Lectures & Courses

From the standpoint of oral health and general health, educate dental hygienists who can contribute to people’s
health and welfare by teaching the knowledge and skills necessary for oral health care centering on oral medicine
education.
(4)

Clinical Services & Other Works

Oral care department, in cooperation with each outpatient in the dental school attached hospital, in order tomaintain and improve the general health through oral health, do patients’ dental preventive measures and oral

― 504 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology

health education in oral care outpatient. In addition, we do oral care for hospitalized patients in the dentistry
department and medical hospital aﬃliated hospitals, we receive consultation about patient oral care from ward
nurses, and instruct oral care methods according to patient condition.
In oral surgery unit, we are engaged in diagnosis, treatment, oral health guidance etc of various oral disease
patients.
(5)

Clinical Performances

Based on knowledge of oral medicine through oral care department, we are conducting perioperative oral function
management with more specialized expertise.

― 505 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology

Preventive Oral Health Care Sciences

Professor
Kayoko SHINADA
Assistant Professor
Hiromi OTSUKA
Part-time lecturer Atsushi OHYAMA
Chiyoko HAKUTA
Graduate Students Master Course
Tomomi ABE,
Makoto KAWANO,
Chie YOSHIZU,(1)

OutlineIn order to cultivate students’ abilities to prevent and detect oral diseases at an early stage, which are important
to maintain and improve the nation’s health, we help students acquire deep academic knowledge and high standard skills in preventive oral health care such as skills to check over the condition of oral cavities. Additionally,
we help students develop skills to provide oral health counseling and oral health promotion, and nurture humanresources who can actively contribute the development of oral health promotion.
(2)

Research

1) Preventive Oral Health Care Sciences① Incident factors and preventive methods on dental caries② Incident factors and preventive methods on periodontal disease③ Incident factors and preventive methods on oral malodor④ Incident factors and preventive methods on other oral diseases2) Development of education system for the patients to prevent oraldiseases and for dental hygiene students.
3) Development of new assessment programs in technical education for
dental hygienist students.

(3)

Clinical Services & Other Works

In our Oral Health Care Clinic, dental hygienists support patients’ oral health care, and prevent dental caries
and periodontal diseases, for the patients to maintain their oral health for the entire lifetime.
(4)

Publications[Original Articles]
1. Hiromi O, Keiko K, Yuki O, Masayo Y, Natsuki K, Masayo S, Keiko E, Shinichi A, Atsuhiro K, Kayoko S.
An inter- and intraprofessioal education program in which dental hygiene students instruct medical anddental students Journal of Dental Education. 2016.09; 80(9); 1062-1070― 506 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology[Conference Activities & Talks]

1. Naito M,Shinada K,Kunitsuka K,Yamamoto R,Onishi T,Nishi M,Seki N,Morio I,Taniyama K. Intervention
survey on oral health at newspaper printing factories. 89th Annual Meeting of the Japan Society for
Occupational Health 2016.05.27 Fukushima city
2. Moross J, Seki N, Sunaga M, Otsuka H, Naito M, Shinada K, Morio I, Kinoshita A. Development of ESL
clinical simulation teaching materials for dental hygienist education. Annual Meeting of the 35th Japanese
Dental Education Association 2016.07.02 Osaka, Japan
3. Kanako Toda, Tomomi Abe, Makoto Kawano, Chie Yoshizu, Hiromi Ohtsuka, Isao Minami, Yoshihiro
Ogawa, Koji Araki, Kayoko Shinada. The eﬀect of oral health education to body and oral health withi
diabetic patients. The 11th Annual Meeting of Japan Dental Hygienists’Association 2016.09.18 Hiroshima
4. Kanako Toda, Keiko Matsuo, Mayuka Taga, Hitomi Arimatsu, Takuo Ishii, Masayoshi Hukushima, Yuichi
Ohshima, Yoshinori Toriyama. Research discussion on "Interprofessinal work". The 11th Annual Meetingof Japan Dental Hygienists’Association 2016.09.19 Hiroshima
5. Yoko Ishii, Kanako Toda, Yuka Shizuma, Hiroko Mega, Kayoko Shinada. Follow-up survey of oral environment and oral health situation in infants. The 75th Annual Meeting of Japanese Society of Public
Health 2016.10.26 Osaka
6. Seki N, Moross J, Otsuka H, Sunaga M, Naito M, Takatsuna Y, Kondo K, Shinada K, Morio I, Kinoshita
A. Learning outcome provided by ESL clinical simulation teaching materials for dental hygiene education.
81st Annual Meeting of the Stomatological Society 2016.11.26 Tokyo
7. Tomomi Abe, Makoto Kawano, Hiromi Otsuka, Kayoko Shinada. Eﬀects of oral care and function improvement program on nursing care elderly. The 81th Stomatological Society Meeting 2016.11.26 Tokyo
8. Kawano Makoto, Abe Tomomi, Yoshizu Chie, Toda Kanako, Otsuka Hiromi, Shinada Kayoko. Survey
on educational present condition and dental hygiene process. The 81th Stomatological Society Meeting2016.11.26― 507 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology

Oral Hearth Sciences for Community Welfare

Professor Junichi FURUYA
Junior Associate Professor Keiko ENDO
Graduate Student Michiyo OBANA
Graduate Student Chiaki MATSUBARA
Graduate Research Student Junji TOKUNAGA(1)Outline

The role of Department of Oral Health Sciences for Community Welfare is to develop education, practice,research for people who can play an important role in medical care and welfare of super-aging society. The
department is particularly focusing on improving oral health such as mastication, swallowing, and oral hygiene
through dysphagia rehabilitation, diet modiﬁcation support, multi-disciprinaly team approach so that the department contributes to prevent and improve aspiration pneumonia, malnutrition, and quality of life.
All educational and research activities are based on clinical practice and experiences so that knowledge and skillsof oral function will be acquired. Concretely, oral functional rehabilitation and oral hygiene care are performed
as oral health management for hospitalized and institutionalized patients, and out patient in clinic.In addition,
we supply multi-disciplinary team approach as a member of NST (Nutrition Support Team), PCT (Palliative
Care Team), Visiting Dysphagia Rehabilitation Team in medical hospital of TMDU.

(2)

Research

1. Oral hygiene, dysphagia rehabilitation, and diet modiﬁcation support in medical care with multi-disciplinary
team approach.
2. Welfare, well-being and life support for people with dysphagia.
3. Oral function, nutrition, and enjoyment of eating in older people.
4. Professional oral hygiene care by dental hygienists
5. Collaboration of medical care, health, nursing-care, and welfare.

(3)

Education

Gerodontology
Welfare for older people
Nursing-care for older people
Prosthodontics
Home visiting dentistry
Community dental care
Social worketc

― 508 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology(4)

Publications[Original Articles]
1. S Onodera, J Furuya, H Yamamoto, Y Tamada, H Kondo. Eﬀects of wearing and removing dentures on
oropharyngeal motility during swallowing. J Oral Rehabil. 2016.11; 43(11); 847-854[Conference Activities & Talks]
1. S Onodera, J Furuya, Y Tamada, H Yamamoto, T Sato, A Hara, A Itsukaichi, S Aki, H Kondo. Impacts
of wearing dentures on oro-pharyngeal movement during pharyngeal swallowing . Twenty-fourth Annual
Meeting of the Dysphagia Research Society 2016.02.26 Tuscon, Arizona, the USA
2. Junichi Furuya. Functional Occlusal Scheme for complete dentures. Peking University Hospital of Stomatology First Clinical Division 2016.11.05 Beijing
3. CMatsubara, K Endo, M Obana, J Furuya. Relationships among experience of health education, oral
Function, and oral health-related quality of life in community-dwelling older individuals. The 81st meetingof Stomatological Society 2016.11.25 Tokyo― 509 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology

Oral Health Care Education

Associate Professor
Keiko Endo
Junior Associate Professor Yuki Ohara(1)

Research

1) Education for dental hygienist students
2) Oral health promotion program3) Development of dental hygiene process(2)

Education

Oral health care education is special ﬁeld of study which deals with establishment of theoretid and skill for
health promotion to contribute to the development of the health. Educational objects of Oral health careeducation in the graduate course is to foster human resources who will be able to implement health promotion
program in collaboration with other career or residents in many ﬁelds.
(3)

Clinical Services & Other Works

In oral health care clinic，dental hygienists support patients oral health care，and prevent dental caries andperiodontal diseases for the patients to maintain the their oral and general health in the entire lifetime．
(4)

Publications[Original Articles]
1. Yuki Ohara, Hirohiko Hirano, Hideyo Yoshida, Shuichi Obuchi, Kazushige Ihara, Yoshinori Fujiwara, Shiro
Mataki. Prevalence and factors associated with xerostomia and hyposalivation among community-dwellingolder people in Japan. Gerodontology. 2016.03; 33(1); 20-27
2. Daisuke Takagi, Hirohiko Hirano, Yutaka Watanabe, Ayako Edahiro, Yuki Ohara, Hideyo Yoshida,
Hunkyung Kim, Kohji Murakami, Shouji Hironaka. Relationship between skeletal muscle mass and swallowing function in patients with Alzheimer’s disease. Geriatr Gerontol Int. 2016.05;
3. Chiyoko Hakuta, Ayako Okada, Kaoru Sogabe, Erika Kakuta, Keiko Endo, Susumu Imai, Masaaki
Okamoto, Yoshiaki Nomura,Nobuhiro Hanada. Opportunistic Bacteria in Tonsil and Dental Plaque are
Indicator for Oral Care Opportunistic Bacteria in Tonsil and Dental Plaque are Indicator for Oral Care.
2016.05; 2(1); 30-34
4. Shiho Morishita, Yutaka Watanabe, Yuki Ohara, Ayako Edahiro, Emiko Sato, Takeo Suga, Hirohiko
Hirano. Factors associated with older adults’ need for oral hygiene management by dental professionals.
Geriatr Gerontol Int. 2016.08; 16(8); 956-962
5. Yuki Ohara, Naomi Yoshida, Hisashi Kawai, Shuichi Obuchi, Hideyo Yoshida, Shiro Mataki, Hirohiko
Hirano, Yutaka Watanabe. Development of an oral health-related self-eﬃcacy scale for use with older
adults. Geriatr Gerontol Int. 2016.08;― 510 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology

6. Yutaka Watanabe, Hirohiko Hirano, Hidenori Arai, Shiho Morishita, Yuki Ohara, Ayako Edahiro, Masaharu Murakami, Hiroyuki Shimada, Takeshi Kikutani, Takao Suzuki. Relationship Between Frailty andOral Function in Community-Dwelling Elderly Adults. J Am Geriatr Soc. 2016.09;
7. Hiromi Otsuka, Keiko Kondo, Yuki Ohara, Masayo Yasuda, Natsuki Kishimoto, Masayo Sunaga, KeikoEndo, Shinichi Arakawa, Atsuhiro Kinoshita, Kayoko Shinada. An Inter- and Intraprofessional Education
Program in Which Dental Hygiene Students Instruct Medical and Dental Students. J Dent Educ. 2016.09;
80(9); 1062-1070[Misc]
1. Hunkyung Kim, Hirohiko Hirano, Ayako Edahiro, Yuki Ohara, Yutaka Watanabe, Narumi Kojima, Miji
Kim, Erika Hosoi, Yuko Yoshida, Hideyo Yoshida, Shoji Shinkai. Sarcopenia: Prevalence and associatedfactors based on diﬀerent suggested deﬁnitions in community-dwelling older adults. Geriatr Gerontol Int.
2016.03; 16 Suppl 1; 110-122
2. Problems and the attempt to improve patients safety education at dental hygiene education in our univerisity 2016.05; 7(1); 39-44[Conference Activities & Talks]
1. Yuki Ohara, Hirohiko Hirano, Yutaka Watanabe, Ayako Edahiro,Shiho Morishita, Maki Shirobe, KeikoEndo. Risk factors associated with aspiration in older persons requiring long-term care: An investigation
with a 2-year follow-up. The 12th International Conference of Asian Academy of Preventive Dentistry2016.05.27 Tokyo
2. Shino Suma, Yutaka Watanabe, Hidenori Arai, Kenji Matsushita, Takashi Sakurai, Hirohiko Hirano,
Ayako Edahiro, Yuki Ohara. Diﬀerential factors aﬀect the appetite in AD and MCI patients. The 12th
International Conference of Asian Academy of Preventive Dentistry 2016.05.27
3. Yuki Ohara. Implication of oral function for frailty and disability. 2nd Asian Conference for frailty andsarcopenia Asian Aging Forum 2016.11.04 Nagoya― 511 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology

Basic Sciences of Oral Health Care

Junior Associate Professor Yujiro Sakamoto(1)

OutlineGraduate School of Medical and Dental Sciences has been reorganized in April 2012, and the section of Basic
Sciences of Oral Health Care was established in Medical and Dental Science and Technology master s program
course.
(2)

Research

1) Basic medical and dental studies for oral health care2) Basic study on clinical application of oral health care3) Gross anatomical study of head and neck region(3)

Education

Purpose of Education
Basic sciences of oral health care is a branch of morphological sciences, developmental biology, pathology and theneurosciences to understand the structure and function of human body and its pathological conditions. Students
are taught in more detail about the normal tooth anatomy and occlusal function as well as the anatomy of thehead and neck with speciﬁc attention to the skull, muscles, nerves, and arteries associated with the mouth andteeth. In addition, students are also taught the oral pathology and dental pharmacology and pharmaceutics.
Subjects and contents.
• Structure and function of human body I and II: anatomy, histology, physiology, embryology, oral anatomy,
oral histology, oral physiology.
• Mechanism of disease and promotion of recovery process: pathology, oral pathology, microbiology, immunology, pharmacology.
• Dental Pharmacology and Pharmaceutics.
• Graduation thesis:(4)

Publications[Books etc]
1. Sakamoto Y. 26, Pharyngeal muscles; 28, Prevertebral and craniocervical junction muscles. In: Bergman’sComprehensive Encyclopedia of Human Anatomic Variation. Wiley-Blackwell, 2016 (ISBN : 978-1-11843035-4)
2. Sakamoto Y. 26, Pharyngeal muscles; 28, Prevertebral and craniocervical junction muscles. In: Bergman’sComprehensive Encyclopedia of Human Anatomic Variation (E-book). Wiley-Blackwell, 2016 (ISBN :978-1-118-43030-9)― 512 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology[Conference Activities & Talks]

1. Sakamoto Y. Gross anatomical observations on the conﬁguration of the extrinsic muscles of the tongue.
The 33rd annual meeting American Association of Clinical Anatomists. 2016.06 Oakland, USA.

― 513 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology

Basic Oral Health Engineering

Professor Kazuhiro Aoki
Associate Professor
Meiko Oki
Assistant Professor
Shingo Kamijoh(1)

OutlineBasic Oral Health Engineering is a department assigned to basic science ﬁeld of oral health in three mastercourse departments which were reorganized from the departments of Oral Health Engineering Course in 2015.
The department is originated in Basic Oral Health Sciences.
The department of Basic Oral Health Engineering aims to create a scientiﬁc foundation for the clinical applications based on interdisciplinary research between engineering and biology and/or interface studies between
basic and clinical sciences. We believe that these research activities, which are focused on the region of the oralcavity, can contribute to attaining healthy and happy living conditions. We have the responsibility to train ourstudents to be medical personnel who are eager to contribute to people’s happiness through a broad range ofeducational courses from basic level courses to professional level courses, which integrate areas of study such as
the structure and function of the human body, stomatognathic region, pharmacology and the research process.
(2)

ResearchResearch Subjects
1) The development of surface coating materials for the prevention of plaque growth2) Research related to the connection between oral bacteria and systemic health3) The development of non-invasive methods of bone mass augmentation
4) The fabrication of facial prostheses using a three-dimensional rapid manufacturing method
5) Clinical studies of treatments for patients with maxillofacial defects
6) The development of materials for facial prostheses
7) The relationship between "medical care to support life" and the dental technician
8) The education of dental technicians using computer simulation training(3)

Lectures & Courses

Basic Oral Health Engineering is a department of oral health engineering which deals with the basic oral healthsciences to perform evidence-based oral health care and prosthetic treatments to support people to promoteoral health and improve quality of life. Main objective of Basic Oral Health Engineering in the undergraduate
course is to provide students opportunity to study the structure and function of the human body, fabricationof dental and maxillofacial prostheses, dental CAD/CAM technology and research process.
(4)

Clinical Performances

Clinical activities· Maxillofacial prosthetic rehabilitation for patients with maxillofacial defects· Making dental and maxillofacial prostheses― 514 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology(5)

Publications[Original Articles]
1. Imura Hiroko, Shimada Masahiko, Yamazaki Yoko, Sugimoto Kumiko. Characteristic changes of saliva
and taste in burning mouth syndrome patients Jouranal of Oral Pathology and Medicine. 2016.03; 45(3);231-236
2. Shizuka Tanaka, Naoko Uehara, Natsumi Tsuchihashi, Kumiko Sugimoto. Emotional relationships between child patients and their mothers during dental treatments Journal of Dental Sciences. 2016.03; 11;287-292
3. Meiko Oki, Tetsuya Suzuki, Hidekazu Takahashi. A modiﬁed indirect method for fabricating silicone
soft-lined complete dentures Journal of Prosthetic Dentistry. 2016.07;
4. Oki M, Kanazaki A, Taniguchi H. Osteoradionecrosis following carbon ion radiotherapy: Case history
report of a soft palate defect. International Journal of Prosthodontics. 2016.09; 29(5); 448-450
5. Taki Sekiya, Hiroko Imura, SachikoIto, Michiyo Miyashin, Kumiko Sugimoto . Development of real-time
stress monitoring system in pediatric dentistry: Assessment from autonomic nerve and brain activities
The Japanese Journal of Pediatric Dentistry. 2016.12; 54(4); 443-449[Conference Activities & Talks]
1. Hiroji Shimomura, Kiyoko Kanamori, Yasuko Kawakami, Kumiko Sugimoto, Ayako Kubota. Survey oforal health status and behavior of medical university students. Collaborative Project Workshop 2015 ofBunkyo Gakuin University Research Institute 2016.05.07 Tokyo
2. Yoko Kono, Ayako Kubota, Mika Matsushima, Masato Taira, Kumiko Sugimoto. Eﬀect of intraoral
stimulation with capsaicin on salivary secretion and neural activity. 17th International Symposium on
Olfaction and Taste 2016.06.05 Yokohama
3. Yu-mi TSUCHIDA，Yuji KABASAWA，Namiaki TAKAHARA，Shingo, KAMIJO，Tetsuya SUZUKI.
Accuracy of a 3D Model for Surgical Support in Orthognathics. Advanced Digital Technology (ADT)
North America & Japan Regional Leadership Groups Conference 2016.06.17 San Diego California USA
4. Kayo Kimura, Kazuo Shimazaki, Kumiko Sugimoto, Takashi Ono. Inﬂuence of habitual mouth-breathingon taste sensation. 94th General Session and Exhibition of The IADR 2016.06.22 Seoul
5. Ayako Kubota, Yoko Kono, Hiroko Imura, Hiroji Shimomura, Kiyoko Kanamori, Yasuko Kawakami,
Shiro Mataki, Kumiko Sugimoto. Oral health status and behavior of Japanese university students. .
International Symposium on Dental Hygiene 2016 2016.06.23 Basel
6. Kumiko Sugimoto, Naomi Yoshida, Yoko Yamazaki, Hiroko Imura, Yoko Kono, Ayako Kubota, Sato
Yamanaka, Hiroyuki Sakamaki, Asako Kimura. Objective assessment of oral and holistic health in dental
hygienists at menopausal stage. International Symposium on Dental Hygiene 2016 2016.06.23 Basel
7. Naomi Yoshida, Kumiko Sugimoto, Sato Yamanaka, Hiroyuki Sakamaki, Yoko Yamazaki, Asako Kimura,
Hiroko Imura, Yoko Kono, Ayako Kubota. Oral and holistic health evaluation and dental health status
in dental hygienists at menopausal stage. International Symposium on Dental Hygiene 2016 2016.06.23
Basel
8. Ayako Kubota, Yasunari Miyazaki, Yoshimi Sakurai, Shiro Mataki, Kumiko Sugimoto. Cross-sectional
study on oral health of university students. Japan Dental Hygienists’ Association The 11th Academic
Meeting 2016.09.17 Hiroshima
9. Ai Hasegawa, Hiromi Otsuka, Kumiko Sugimoto. Investigation of inﬂuences of habitual mouth-bleathingon halitosis and lip function. The 11th Academic Meeting of Japan Dental Hygienists’ Association
2016.09.17 Hiroshima― 515 ―

Medical and Dental Science and Technology

Medical and Dental Science and Technology

10. Ayako Kubota, Yasunari Miyazaki, Yoshimi Sakurai, Shiro Mataki, Kumiko Sugimoto. Cross-sectional
survey of oral healthcare for university students in Kanto-Koshinetsu District Association . The 54th
Annual Meeting of Japan University Health Association 2016.10.05 Osaka
11. Tamaki Hada, Hidekazu Takahashi, Shingo Kamijo, Masaomi Ikeda, Toshio Kitamura, Shizuo Higuchi,
Tetsuya Suzuki. EFFECT OF CAD/CAM FABRICATED FRAMEWORK ON COMPLETE DENTURE
DEFORMATION. ACADEMY OF DENTAL MATERIALS (ADM) Annual Meeting 2016.10.12 Chicago,
IL, USA12. Yui Kasai, Kumiko Sugimoto, Shingo Kamijo, Meiko Oki. Investigation on the role of dental technicians
among the dentists practicing home-visit dental care. The 81th Annual Meeting of the StomatologicalSociety 2016.11.26 Tokyo
13. Kumiko Sugimoto. Mechanisms undelying the function of eating. The 7th Annual Meeting of Japan
Society of Dental Hygiene Education 2016.12.10 Tokyo― 516 ―

MedicalMedicalandandDental
DentalScience
Science andand Technology
Technology

Oral Biomaterials Development Engineering

Professor
Junior Associate Professor
Assistant Professor

Hidekazu TAKAHASHI
Tohru YASUE
Naohiko IWASAKI

Graduate student (Master cource) Yuko NAKAJIMA
Special research student Sasipin LAUVAHUTANON (April to May, Chulalongkorn Univ)
Reaserch student Chen Hsuan (July, Taipei Medical Univ.)
Reaserch student
Lin, Jhih-Ni (July, Taipei Medical Univ.)(1)

OutlineBasic knowledge of dental materials and devices for oral health engineering are provided for student. Basic
excerise for dental materials and prosthetic traing are also provided.
Development and evaluation of new dental materials are preformed.

(2)

Research

1. Evaluation of various factors on mechanical properties of teeth substance.
2. Evaluation of fatigue properties of dentin and dental materials using miniature testing pieces
3. Measurement of characteristics of dental ceramic materials and establishment of new testing methods fordental ceramics
4. Measurement of precise deformation using non-contact methods
5. Development of new composite resin with similar machinability of dentin
6. Study on dental root fracture mechanism
7. Applicayion of various types of ﬁberglass for dentistry
8. Evaluation of composite resin mechanical properties and improvement their bonding eﬃciency to various
materials.
9. Evaluation of impact force absorption of mouthguard and face protect materials(3)

Education

Dental material science is not only one of basic medical and dental science but also one of clinical dental science.
In our department, we will educate students to obtain practical knowledge of the dental materials and devices
used in dentistry and to improve skill how to deal with these materials and devices. Our goals of education are
to achieve high quality of dental practice with well-understanding dental material and devices.
The aim for education is to obtain the basic knowledge of dental material science and technology. The lecture
is simultaneously provided with the laboratory instructions within the limit of the possible.

― 517 ―

Medical andand Dental
Dental Science
Science andand Technology
Technology
Medical(4)

Lectures & Courses

Dental material science is not only one of basic medical and dental science but also one of clinical dental science.
In our department, we will educate students to obtain practical knowledge of the dental materials and devices
used in dentistry and to improve skill how to deal with these materials and devices. Our goals of education are
to achieve high quality of dental practice with well-understanding dental material and devices.
The aim for education is to obtain the basic knowledge of dental material science and technology. The lecture
is simultaneously provided with the laboratory instructions within the limit of the possible. Presentation not
only domestic but also international meeting is strongly encouraged.
(5)

Clinical Services & Other Works

Participatation in various congresses are strongly recommended. Assistance for standard publication is also
cooporated. Especially, Prof. Takahashi, head of Oral Biomaterials Engineering acts as the chairperson of ISO
TC106 Dentistry/SC9 Dental CAD/CAM systems for publishing ISO standards.
(6)

Publications[Misc]
1. Hidekazu TAKAHASHI. Outlook of CAD/CAM resin blocks approved by social insurance system Journalof Japan Academy of Digital Dentistry. 2016.07; 6(1); 3-6[Conference Activities & Talks]
1. Hidekazu TAKAHASHI. General inofmation for CAD/CAM composite resin blocks.. Project seminar ofJapanese Academy of Digital Dentistry 2016.02.21 Tokyo
2. Takahashi H, Iwasaki N, Suzuki T. Flexural properties of recent composite resin blocks for CAD/CAM.
2nd meeting of International Academy of Digital Dental Medicine 2016.09.03 Busan
3. Nakajima Y, Iwasaki N, Takahashi H, Yoshida M, Honda E, Kurabayashi T. MRI Artifacts and Radiopacity of CAD/CAM Composite Resin Blocks. The 2016 Academy of Dental Materials Annual Meeting2016.10.13 Chicago
4. Takahashi H, Iwasaki N, Sumi Y. Eﬀect of Cavity Design on Gap Formation Observed by OCT. The 2016
Academy of Dental Materials Annual Meeting 2016.10.14 Chicago
5. Takahashi H, Iwasaki N, Yasue T, Sumi Y. Observation of dental restoration gap formation using optical coherence tomography. 1st International Symposium on Creation of Life Innovation Materials forInterdisciplinary and International Researcher 2016.10.17 Suita, Osaka, Japan[Awards & Honors]
1. Best Poster Award (3rd prize), International Academy for Digital Dental Medicine, 2016.09― 518 ―

MedicalMedicalandandDental
DentalScience
Science andand Technology
Technology

Oral Prosthetic Engineering

Professor Tetsuya SUZUKI
Junior Associate Professor Masaomi IKEDA
Research Associate Kouichi FUKAWA(1)

OutlineOral Health Information Technology educates deepen understanding of the production of the dental prosthesis
using the latest computer science and cultivate basics power to new technology development. This course
cultivates the ability to oﬀer high quality medical technology taking advantage of expertise or knowledge.
And the purpose of this section is to educate professional dental technologists who has ability to apply newly
developed materials and technologies and who is able to contribute in not only clinical situation but also
research institution or educational organization at international levels. Presently, the latest technologies suchas dental implant and dental CAD/CAM etc became popular by the development of materials and the progressin technologies among dental treatment. Therefore, it is necessary to understand and lean knowledge about
newly developed materials and technologies for properly control the dental laboratory works. Furthermore, It isnecessary that the communication skill for report information about the materials and technologies to dentists
and dental hygienists. Based on these evidences, it is an education in which specialists are raised to not bebound by classiﬁcation as technologist and have ambition.

(2)

Research

1) The advanced technology which utilized a CADCAM system.
2) The education of dental technician which utilized computer simulation training.
3) Relation of "medical care to support life" and the dental technician.
4) Evaluation of newly developed materials.

(3)

Education

Introduction of Oral Health Engineering, Introduction of Clinical Dental Technology, Formative Arts Practice,
Basic Technology of Manufacturing, Teeth Morphological Curving, Advanced Teeth Morphological Curving,
Conservative Dentistry, Science of Occlusion, Advanced Science of Occlusion, Communication Theory, Health
Promotion, Basic Fixed Prosthodontics, Advanced Fixed Prosthodontics, Complete Denture Prosthodontics, Removable Partial Prosthodontics, Oral Rehabilitation Engineering, Laws for Dental Technologists, CAD/CAM
System Technology, CAD/CAM System Technology Practice, Advanced Technology for Oral Health, Ceramic
Processing Technology Practice, Management and Regulation for Dental Technologists, Pediatric Dental Technology, Orthodontic Dental Technology, Maxillofacial Prosthetics Technology, Digital Image Processing Practice,Oral Appliances, Comprehensive Oral Rehabilitation Engineering Practice, Graduation Research, Graduation
Product― 519 ―

Medical andand Dental
Dental Science
Science andand Technology
Technology
Medical(4)

Publications[Original Articles]
1. Fang-Yu Fan , Chung-Chieh Chang , Jhih-Ni Lin , Yi-Yun Cai , Chien-Yie Tsay ,Tetsuya Suzuki , Hidekazu
Takahashi , Yung-Ning Pan , Chung-Kwei Lin. Characterization of bioactive glass coated carbon nanotubes prepared by sol-gel process International Journal of Applied Glass Science. 2016;
2. Tezuka H, Shimada Y, Matin K, Ikeda M, Sadr A, Sumi Y, Tagami J.. Assessment of cervical demineralization induced by Streptococcus mutans using swept-source optical coherence tomography. J Med
Imaging. 2016.01; 3(1);3. Shinagawa J, Inoue G, Nikaido T, Ikeda M, Sadr A, Tagami J.. Dentin bonding performance and interface
observation of an MMA-based restorative material. Dent Mater J.. 2016.07; 35(4); 578-584
4. Meiko Oki, Tetsuya Suzuki, Hidekazu Takahashi. A modiﬁed indirect method for fabricating silicone
soft-lined complete dentures Journal of Prosthetic Dentistry. 2016.07;
5. Horie K, Shimada Y, Matin K, Ikeda M, Sadr A, Sumi Y, Tagami J.. Monitoring of cariogenic demineralization at the enamel-composite interface using swept-source optical coherence tomography. Dent Mater..
2016.09; 32(9); 1103-1112
6. Suguru Kimoto, Yasuhiko Kawai, Atsuko Gunji, Hisatomo Kondo, Taro Nomura, Tomohiko Murakami,
Akito Tsuboi, Guang Hong, Shunsuke Minakuchi, Yusuke Sato, Gaku Ohwada, Tetsuya Suzuki, Katsuhiko
Kimoto, Noriyuki Hoshi, Makiko Saita, Yoshikazu Yoneyama, Yohei Sato, Masakazu Morokuma, Joji
Okazaki, Takeshi Maeda, Kenichiro Nakai, Tetsuo Ichikawa, Kan Nagao, Keiko Fujimoto, Hiroshi Murata,
Tadafumi Kurogi, Kazuhiro Yoshida, Masahiro Nishimura, Yasuhiro Nishi, Mamoru Murakami, Toshio
Hosoi,Taizo Hamada. Study protocol for a multi-center, randomized controlled trial to develop Japanese
denture adhesive guidelines for patients with complete dentures: the Denture Adhesive Guideline trial:
study protocol for a randomized controlled trial Trials. 2016.10; 17;[Conference Activities & Talks]
1. Yuki Naruse, Tomohiro Tkakagaki, Naoko Matsui, Takaaki Sato, Toru Nikaido, Masaomi Ikeda, Junji
Tagami. Eﬀects of alumina-blasting for a CAD/CAM resin block on adhesion. 45th Annual meeting ofAmerican Association for Dental Research 2016.03.17 Los Angels
2. Arakida T,Kanazawa M,Yamamoto S,Iwaki M, Minakuchi S,Suzuki T. The eﬀect if external light of digital
impression. The eﬀect of external light of digital impression 2016.05.29
3. Microtensile bond strength to dentin of a newly developed one-step self-etch system containing hydrophilic
amide monomer. 2016.06.09
4. Yu-mi TSUCHIDA，Yuji KABASAWA，Namiaki TAKAHARA，Shingo, KAMIJO，Tetsuya SUZUKI.
Accuracy of a 3D Model for Surgical Support in Orthognathics. Advanced Digital Technology (ADT)
North America & Japan Regional Leadership Groups Conference 2016.06.17 San Diego California USA
5. Multicenter Randomized Clinical Study of Denture Adhesive to Establish the Guideline: Study by the
Subgroup Analysis for a Denture Adhesive on the Eﬀect of the Masticatory Performance. 2016.07.09
6. Arakida T,Kanazawa M,Yamamoto S,Iwaki M, Minakuchi S,Suzuki T,Oki M,Kamijo S,Ando K,Morisawa
K. The eﬀect of external light of digital impression time and accuracy. 2016.07.09― 520 ―

Endowed
Endowed Departments
Departments

Department of Nanomedicine

Lecturer Kengo Iwasaki
Associate Professor Motohiro Komaki
Project researchers Kenichi Morinaga (〜2017.3.31(1)

Naoki Yokoyama)

OutlineDepartment of Nanomedicine has been established as endow courses in 2005 by a suggestion of Ikuo Morita(Excutive director and Excutive vice president of Research and International Cooperation) and ﬁnancing of
Dai Nippon Printing Co., Ltd (DNP). We work on a team from Medical, Dental and Science to conduct basic
researches of oﬀ-the-shelf regenerative therapies using stem cells or their derivatives.
(2)

ResearchMesenchymal stem cells residing within tissues are a promising source of cells for regenerative therapy due
to their multi-diﬀerentiation and high proliferative potency. We study to develop safer and more eﬀective
therapeutic application of stem cells. We study novel periodontal regenerative therapy by using cell-transfer
and -patterning technique. Also we study cell-free system (stem cell-conditioned medium and extracellular
vesicles)for various disease.
(3)

Lectures & Courses

In the lecture for periodontolgy（D4, D5）, we learn biological principle necessary for periodontal regenerationand the basics of periodontal surgical procedure. In graduate school lecture, we understand a mechanism ofwound healing and a principle of therapeutic potential of stem cells through the latest ﬁndings such as woundhealing-stimulating activity by the humoral factor secreted by stem cells. Also, we introduce novel intercellularcommunication vehicle as such extra cellular vesicles (exosomes), transfer and patterning technique and give an
outline about application of stem cells to tissue engineering.
(4)

Clinical Services & Other Works

In dental hospital(Periodontics), Drs. Komaki and Iwasaki as periodontal specialists and preceptors oﬀer dental
treatments of the highest standard in accord with the needs of patients and clinical training of students.
(5)

Publications[Original Articles]
1. Tooi M, Komaki M, Morioka C, Honda I, Iwasaki K, Yokoyama N, Ayame H, Izumi Y, Morita I. Placenta
Mesenchymal Stem Cell Derived Exosomes Confer Plasticity on Fibroblasts. J. Cell. Biochem.. 2016.07;
117(7); 1658-1670
2. Noda M, Aoki A, Mizutani K, Lin T, Komaki M, Shibata S, Izumi Y. High-frequency pulsed low-level
diode laser therapy accelerates wound healing of tooth extraction socket: An in vivo study. Lasers inSurgery and Medicine. 2016.07; 48(10); 955-964― 521 ―

Endowed Departments
Departments
Endowed3. Keiko Akazawa, Kengo Iwasaki, Mizuki Nagata, Naoki Yokoyama, Hirohito Ayame, Kazumasa Yamaki,
Yuichi Tanaka, Izumi Honda, Chikako Morioka, Tsuyoshi Kimura, Motohiro Komaki, Akio Kishida, Yuichi
Izumi, Ikuo Morita. Double-layered cell transfer technology for bone regeneration. Scientiﬁc Reports.
2016.09; 6; 33286[Conference Activities & Talks]
1. Komaki M, Aoki A, Iwasaki K, Izumi Y. Coverage of class III gingival recession localized at anterior
mandibular teeth using connective tissue graft case report. 59th Spring Meeting for Japanese Society of
Periodontology 2016.05.19 Kagoshima
2. Komaki M, Aoki A, Iwasaki K, Izumi Y. Coverage of class III gingival recession localized at anterior
mandibular teeth using connective tissue graft. The 59th Spring Meeting of The Japanese Society of
Periodontology 2016.05.21
3. Tooi M, Komaki M, Iwasaki K, Yokoyama N, Nagata M, Izumi Y, Morita I. Placenta mesenchymal stem
cell derived exosomes alter diﬀerentiation competence in ﬁbroblasts. The 144th Meeting of the JapaneseSociety of Conservative Dentistry 2016.06.09 Utsunomiya
4. Komaki M, Tooi M, Iwasaki K, Morita I. MSC exosomes alter conpetency of ﬁbroblasts to diﬀerentiation
stimuli. The 34th Annual Meeting of the Japanese Society for Bone and Mineral Research 2016.07.20
Osaka
5. Komaki M, Tooi M, Iwasaki K, Morita I . Mesenchymal stem cell derived exosomes alter diﬀerentiationcompetence in ﬁbroblasts. The 34th Anuual Meeting of the Japanese Society for Bone and Mineral
Research 2016.07.22 Osaka
6. Masahiro Noda, Akira Aoki, Koji Mizutani, Taichen Lin, Motohiro Komaki, Shunichi Shibata, Yuichi
Izumi. Eﬀect of low level laser irradiation on early stage of wound healing of tooth extraction socket. The
23rd General Meeting of the Japanese Association for Dental Science 2016.10 Fukuoka
7. Kengo Iwasaki, Mizuki Nagata, Keiko Akazawa, Naoki Yokoyama, Motohiro Komaki, Ikuo Morita. The
eﬀect of spheroid culture on the expression of stem cell-related genes in periodontal ligament stem cells.
The 145th Meeting of The Japanese Society of Conservative Dentistry 2016.10.28 Matsumoto― 522 ―

Endowed
Endowed Departments
Departments

Department of Liver Disease Control

Professor
Senior Associate Professor

Yasuhiro ASAHINA
Sei KAKINUMA

Graduate Student(collaboration with Department of Gastroenterology and Hepatology in TMDU)
Fumio GOTO(-03/2016), Shun KANEKO, Hiroko NAGATA,Yu ASANO,
Emi INOUE, Tomoyuki TSUNODA, Masato MIYOSHI,
Ayako SATO (04/2016-)(1)

OutlinePatients died from chronic liver diseases, including liver cancer, are about 40,000 persons per a year in Japan.
Liver transplantation remains the only eﬀective treatment available to patients with end-stage liver diseases.
Because of a serious shortage of donors for allogeneic liver transplantation, an alternative therapy is needed.
Prevention of hepatocarcinogenesis and hepatic ﬁbrosis is also necessary for patients with chronic hepatitis, andthe development of eﬀective treatment for progressive liver diseases has been quite essential. We believe thatthe central role of clinical departments in the graduate school of TMDU is to establish basis for the innovative
medical treatment in the next generation. To achieve our mission, both basic research lead by clinical concepts
and development of novel therapeutics established upon basic research are required.
Our section is a donation-funded department collaborating with the Department of Gastroenterology and Hepatology in TMDU. Most of basic research projects, education for students, and clinical contributions including
multicenter studies are collaboration with the Department of Gastroenterology and Hepatology in TMDU. The
goal of our education is to promote students to become a well-developed hepatologist, and also a leading expert
in the ﬁeld of Hepatology and Gastroenterology.

(2)

Research

Our principle is to achieve a research evoked from various clinical problems, and also directed to launch innovative therapeutic procedures to the daily clinical practice.
We focus on the basic studies of molecular mechanism regulating development, pathophysiology, progression,and therapeutic resistance of hepatocellular carcinoma (HCC). We also focus on the development of novel disease models using human induced pluripotent stem cells, the research for molecular mechanisms regulating livertissue regeneration and hepatic ﬁbrogenesis, the study for molecular mechanisms regulating diﬀerentiation ofhepatic stem/progenitor cells, analysis of mechanisms regulating escape of hepatitis viruses from innate immune
systems in host cells, and factors for therapeutic resistance against antiviral agents.
Research projects· Research for mechanism regulating development, pathophysiology, progression, and therapeutic resistance of
HCC, based on molecular biology and genome informatics· Development of novel disease models using human induced pluripotent stem cells to elucidate the pathophysiology of liver diseases· Research for molecular mechanisms regulating liver tissue regeneration and hepatic ﬁbrogenesis― 523 ―

Endowed Departments
Departments
Endowed· Research for molecular mechanisms regulating diﬀerentiation of hepatic stem/progenitor cells· Analyses of molecular mechanisms regulating escape of hepatitis viruses from innate immune systems in hostcells, and clinical factors for therapeutic resistance against antiviral agents.

(3)

Education

Primary goal for education in our section is to train highly educated and experienced clinician-researchers inthe ﬁeld of hepatology. Our goal for education of graduate students is to produce clinical researchers thinking
from a wide perspective and to bring up leaders of the next generation in hepatology.
(4)

Lectures & Courses

Our lectures and courses are collaboration with the Department of Gastroenterology and Hepatology in Schoolof Medicine, TMDU. We also educate clinical residents in Medical Hospital of TMDU and graduate students of
the Department of Gastroenterology and Hepatology, in TMDU in collaboration with such department.
(5)

Clinical Services & Other Works

For the treatment of patients with diseases of liver, biliary duct, and pancreas in Medical Hospital of TMDU, we
collaborate with the Department of Gastroenterology and Hepatology in TMDU. In the clinical section, we pursue development and application of highly advanced technologies, including novel procedures, for sophisticateddiagnosis and treatment of diseases of liver, biliary duct, and pancreas. We also operate a lot of multicenter
study collaborating with the Department of Gastroenterology and Hepatology in TMDU. We participate in four
research projects for treatment and eradiation of hepatitis virus funded by Japan Agency for Medical Researchand Development (AMED). We published a lot of studies in peer-reviewed international journals and presented
the recent works in a lot of international and domestic conferences as described below.
(6)

Clinical Performances

For the treatment of patients with diseases of liver, biliary duct, and pancreas in Medical Hospital of TMDU, we
collaborate with the Department of Gastroenterology and Hepatology in TMDU. We have recently establishedthe outpatient department specialized for chronic hepatitis, cirrhosis, and HCC. We have operated a lot of
multicenter study about eﬃcacy of treatment against viral hepatitis, named as Ochyanomizu Liver Conference .
More than 2000 patients with viral hepatitis were enrolled in such studies, and they have clariﬁed the clinicalfactors predicting accurately the therapeutic prognosis and risk for development of HCC. We are developing thescreening programs for the early detection of HCC in patients with chronic hepatitis after eradication of viruses
utilizing non-invasive elastography, novel serum markers, and dynamic contrast-enhanced ultrasonography.
For the treatment of HCC, three-dimensional location and structure of tumors and vessels are evaluated bymultilateral approaches using dynamic contrast-enhanced ultrasonography, Gd-EOB-DTPA enhanced MRI,
and real-time virtual ultrasonography (RVS). We have reported the utility and safety of such therapeutic
approaches. We are providing patients the appropriate therapeutic option based on collective multimodal
therapeutic strategy in collaboration with departments of surgery and radiology.

(7)

Publications[Original Articles]
1. Jun Itakura, Masayuki Kurosaki, Chitomi Hasebe, Yukio Osaki, Kouji Joko, Hitoshi Yagisawa, Shinya
Sakita, Hiroaki Okushin, Takashi Satou, Hiroyuki Hisai, Takehiko Abe, Keiji Tsuji, Takashi Tamada,Haruhiko Kobashi, Akeri Mitsuda, Yasushi Ide, Chikara Ogawa, Syotaro Tsuruta, Kouichi Takaguchi,
Miyako Murakawa, Yasuhiro Asahina, Nobuyuki Enomoto, Namiki Izumi. Complex Pattern of ResistanceAssociated Substitutions of Hepatitis C Virus after Daclatasvir/Asunaprevir Treatment Failure. PLoS
ONE. 2016; 11(10); e0165339― 524 ―

Endowed
Endowed Departments
Departments

2. Yasuhiro Asahina, Mamoru Watanabe. Need for appropriate programs for prevention of hepatitis B
infection: Lessons from the latest nationwide survey in Japan. J. Gastroenterol. Hepatol. 2016.01; 31(1);12-13
3. Satoshi Otani, Sei Kakinuma, Akihide Kamiya, Fumio Goto, Shun Kaneko, Masato Miyoshi, Tomoyuki
Tsunoda, Yu Asano, Fukiko Kawai-Kitahata, Sayuri Nitta, Toru Nakata, Ryuichi Okamoto, Yasuhiro Itsui,
Mina Nakagawa, Seishin Azuma, Yasuhiro Asahina, Tomoyuki Yamaguchi, Naohiko Koshikawa, Motoharu
Seiki, Hiromitsu Nakauchi, Mamoru Watanabe. Matrix metalloproteinase-14 mediates formation of bileducts and hepatic maturation of fetal hepatic progenitor cells. Biochem. Biophys. Res. Commun.
2016.01; 469(4); 1062-1068
4. Yasuhiro Asahina, Namiki Izumi, Kumada Hiromitsu, Masayuki Kurosaki, Kazuhiko Koike, Fumitaka
Suzuki, Hajime Takikawa, Atsushi Tanaka, Eiji Tanaka, Yasuhito Tanaka, Hirohito Tsubouchi, Norio
Hayashi, Naoki Hiramatsu, Hiroshi Yotsuyanagi. JSH Guidelines for the Management of Hepatitis C
Virus Infection: A 2016 update for genotype 1 and 2. Hepatol. Res. 2016.02; 46(2); 129-165
5. Fukiko Kawai-Kitahata, Yasuhiro Asahina, Shinji Tanaka, Sei Kakinuma, Miyako Murakawa, Sayuri Nitta,
Takako Watanabe, Satoshi Otani, Miki Taniguchi, Fumio Goto, Hiroko Nagata, Shun Kaneko, Megumi
Tasaka-Fujita, Yuki Nishimura-Sakurai, Seishin Azuma, Yasuhiro Itsui, Mina Nakagawa, Minoru Tanabe,
Shinichi Takano, Mitsuharu Fukasawa, Minoru Sakamoto, Shinya Maekawa, Nobuyuki Enomoto, Mamoru
Watanabe. Comprehensive analyses of mutations and hepatitis B virus integration in hepatocellular
carcinoma with clinicopathological features. J. Gastroenterol. 2016.03; 51(5); 473-486
6. Miki Taniguchi, Megumi Tasaka-Fujita, Mina Nakagawa, Takako Watanabe, Fukiko Kawai-Kitahata,
Satoshi Otani, Fumio Goto, Hiroko Nagata, Shun Kaneko, Sayuri Nitta, Miyako Murakawa, Yuki NishimuraSakurai, Seishin Azuma, Yasuhiro Itsui, Kenichi Mori, Shintaro Yagi, Sei Kakinuma, Yasuhiro Asahina,
Mamoru Watanabe. Evaluation of Interferon Resistance in Newly Established Genotype 1b Hepatitis C
Virus Cell Culture System. J Clin Transl Hepatol. 2016.03; 4(1); 5-11
7. Seishin Azuma, Yasuhiro Asahina, Yuki Nishimura-Sakurai, Sei Kakinuma, Shun Kaneko, Hiroko Nagata,
Fumio Goto, Satoshi Ootani, Fukiko Kawai-Kitahata, Miki Taniguchi, Miyako Murakawa, Takako Watanabe, Megumi Tasaka-Fujita, Yasuhiro Itsui, Mina Nakagawa, Mamoru Watanabe. Eﬃcacy of additional
radiofrequency ablation after transcatheter arterial chemoembolization for intermediate hepatocellular
carcinoma. Hepatol Res. 2016.04; 46(4); 312-319
8. Shun Kaneko, Sei Kakinuma, Yasuhiro Asahina, Akihide Kamiya, Masato Miyoshi, Tomoyuki Tsunoda,
Sayuri Nitta, Yu Asano, Hiroko Nagata, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako Murakawa, Yasuhiro Itsui, Mina Nakagawa, Seishin Azuma, Hiromitsu Nakauchi, Hironori Nishitsuji, Saneyuki Ujino,
Kunitada Shimotohno, Masashi Iwamoto, Koichi Watashi, Takaji Wakita, Mamoru Watanabe. Human
induced pluripotent stem cell-derived hepatic cell lines as a new model for host interaction with hepatitis
B virus. Sci Rep. 2016.07; 6; 29358
9. Hiroko Nagata, Mina Nakagawa, Yuki Nishimura-Sakurai, Yu Asano, Tomoyuki Tsunoda, Masato Miyoshi,
Shun Kaneko, Fumio Goto, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako Murakawa, Sayuri Nitta,
Yasuhiro Itsui, Seishin Azuma, Sei Kakinuma, Naoko Tojo, Shuji Tohda, Yasuhiro Asahina, Mamoru
Watanabe, . Serial measurement of Wisteria ﬂoribunda agglutinin positive Mac-2-binding protein is
useful for predicting liver ﬁbrosis and the development of hepatocellular carcinoma in chronic hepatitis C
patients treated with IFN-based and IFN-free therapy.[ Epub ahead of print] Hepatol Int. 2016.07;
10. Fumio Goto, Sei Kakinuma, Masato Miyoshi, Tomoyuki Tsunoda, Shun Kaneko, Ayako Sato, Yu Asano,
Satoshi Otani, Seishin Azuma, Hiroko Nagata, Fukiko Kawai-Kitahata, Miyako Murakawa, Sayuri Nitta,
Yasuhiro Itsui, Mina Nakagawa, Yasuhiro Asahina, Mamoru Watanabe. Bone morphogenetic protein-4
modulates proliferation and terminal diﬀerentiation of fetal hepatic stem/progenitor cells. Hepatol. Res.
2016.09;
11. Masato Miyoshi, Sei Kakinuma, Yoko Tanabe, Koji Ishii, Tian-Cheng Li, Takaji Wakita, Yukio Tsuura,
Hideki Watanabe, Yasuhiro Asahina, Mamoru Watanabe, Takaaki Ikeda. Chronic Hepatitis E Infection
in a Persistently Immunosuppressed Patient Unable to Be Eliminated after Ribavirin Therapy. Intern.
Med. 2016.10; 55(19); 2811-2817― 525 ―

Endowed Departments
Departments
Endowed12. Sayuri Nitta, Yasuhiro Asahina, Mami Matsuda, Norie Yamada, Ryuichi Sugiyama, Takahiro Masaki,
Ryosuke Suzuki, Nobuyuki Kato, Mamoru Watanabe, Takaji Wakita, Takanobu Kato. Eﬀects of ResistanceAssociated NS5A Mutations in Hepatitis C Virus on Viral Production and Susceptibility to Antiviral
Reagents. Sci Rep. 2016.10; 6; 34652
13. Hitomi Takada, Kaoru Tsuchiya, Yutaka Yasui, Natsuko Nakakuki, Nobuharu Tamaki, Shoko Suzuki,
Hiroyuki Nakanishi, Jun Itakura, Yuka Takahashi, Masayuki Kurosaki, Yasuhiro Asahina, Nobuyuki
Enomoto, Namiki Izumi. Irregular vascular pattern by contrast-enhanced ultrasonography and high serum
Lens culinaris agglutinin-reactive fraction of alpha-fetoprotein level predict poor outcome after successful
radiofrequency ablation in patients with early-stage hepatocellular carcinoma. Cancer Med. 2016.11;
5(11); 3111-3120
14. Miyako Murakawa, Yasuhiro Asahina, Fukiko Kawai-Kitahata, Mina Nakagawa, Sayuri Nitta, Satoshi
Otani, Hiroko Nagata, Shun Kaneko, Yu Asano, Tomoyuki Tsunoda, Masato Miyoshi, Yasuhiro Itsui,
Seishin Azuma, Sei Kakinuma, Yasuhito Tanaka, Sayuki Iijima, Kaoru Tsuchiya, Namiki Izumi, Shuji
Tohda, Mamoru Watanabe. Hepatic IFNL4 expression is associated with non-response to interferonbased therapy through the regulation of basal interferon-stimulated gene expression in chronic hepatitis
C patients. [ Epub ahead of print] J. Med. Virol. 2016.12;[Conference Activities & Talks]
1. Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyoshi M, Asano Y, Nagata H,
Goto F, Otani S, Kitahata F, Murakawa M , Itsui Y, Nakagawa M, Azuma S, Watanabe M. A Model forInteraction between HBV and Host Cells Derived from Human Induced Pluripotent Stem Cells. The 25th
Asian Paciﬁc Association for the Study of the Liver 2016.02.22 International Convention Center Pamir,Tokyo（JAPAN）
2. Asahina Y. 【HCV 2】Chair . The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22
International Convention Center Pamir, Tokyo（JAPAN）
3. Asahina Y. 【HCV 5】Chair. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22
International Convention Center Pamir, Tokyo（JAPAN）
4. Kakinuma S. 【Basic science】Chair. The 25th Asian Paciﬁc Association for the Study of the Liver2016.02.22 International Convention Center Pamir, Tokyo（JAPAN）
5. Kawai-Kitahata F, Asahina Y, Tanaka S, Kakinuma S, Murakawa M, Nitta S, Otani S, Goto F, Nagata
H, Kaneko S, Asano Y, Miyoshi M, Tsunoda T, Azuma S, Itsui Y, Nakagawa M, Tanabe M, Maekawa
S, Enomoto N, Watanabe M. Comprehensive analyses of mutations and hepatitis B virus integrationin hepatocellular carcinoma. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22
International Convention Center Pamir, Tokyo（JAPAN）
6. Nagata H, Sakurai Y, Nakagawa M, Kakinuma S, Azuma S, Murakawa M, Nitta S, Itsui Y, Asahina Y,
Watanabe M. Serial changes in M2BPGi levels as predictors of ﬁbrosis and HCC in chronic hepatitis C.
The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22 International Convention Center
Pamir, Tokyo（JAPAN）
7. Nitta S, Asahina Y, Wakita T, Kato T.. In vitro characterization and drug sensitivity analysis for HCV
NS5A resistance-associated variants.. The 25th Asian Paciﬁc Association for the Study of the Liver2016.02.22 International Convention Center Pamir, Tokyo（JAPAN）
8. Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyoshi M, Asano Y, Nagata H ,Goto F, Otani S, Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Watanabe M. A new model
for studying interaction between hepatitis B virus and host cells derived from human induced pluripotent
stem cells. EASL The International Liver Congress 2016 2016.04.14 Barcelona（Spain）
9. Asahina Y. Hepatocarcinogenesis after hepatitis C viral eradication.. The 10th APASL Single Topic
Conference 2016.06.08 Kaoshiung（Taiwan）
10. Asahina Y. Recent progress in anti-HCV therapy and challenges toward HCC eradication.. Academic
Forum of Liver Disease in Northeast China and the Annual Convention for the Study of Liver Diseases in
Jilin Province in 2016. 2016.10.29 Changchun（China）― 526 ―

Endowed
Endowed Departments
Departments

11. Asahina Y. 【International Session（Symposium）1】Genomics of hepatocellular carcinoma: Hepatitis
virus infection and hepatocarcinogenesis. Chair . JDDW2016 2016.11.03 Kobe Cinvention Center（KobeJAPAN）
12. Shun Kaneko, Sei Kakinuma, Yasuhiro Asahina, Akihide Kamiya, Masato Miyoshi, Tomoyuki Tsunoda,
Sayuri Nitta, Ayako Sato, Yu Asano, Hiroko Nagata, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako
Murakawa, Yasuhiro Itsui, Mina Nakagawa, Seishin Azuma, Mamoru Watanabe. Genetically modiﬁed
human induced pluripotent stem cell-derived hepatic progenitor-like cell lines as a model for interaction
between hepatitis B virus and host cells. AASLD The Liver meeting 2016 2016.11.11 Boston（USA）
13. Wai-Kay Seto , Yasuhiro Asahina , Cheng-Yuan Peng , Carol Stanciu , Djamal Abdurakhmanov , John F.
Flaherty , Kyungpil Kim , Anuj Gaggar , Mani Subramanian, Fehmi Tabak, Tuan T. Nguyen, Henry LikYuen Chan. Reduced Changes In Bone Mineral Density In CHB Patients Receiving Tenofovir Alfenamide(TAF) Com-pared With Tenofovir disoproxil fumarate. AASLD The Liver meeting 2016 2016.11.11 Boston（USA）― 527 ―

Endowed Departments

Endowed Departments

Department of Advanced Therapeutics for GI Diseases

Professor
Junior Associate Professor
Assistant Professor

Tetsuya NAKAMURA
Katsuyoshi MATSUOKA
Tomohiro MIZUTANI（-03/2016)
Michio ONIZAWA （08/2016-)

Graduate Student
Yuka MATSUMOTO, Shintaro AKIYAMA(1)OutlineThe goal of our department is to develop novel therapeutic strategies for inﬂammatory bowel diseases (IBD) in
humans. With multiple layers of support of corporations who wish to contribute to our mission, we have been
focusing on IBD research from the clinical and basic science perspectives, providing an exceptional education
program for graduate students at TMDU.

(2)

Research

Our research activities focus on the key areas listed below. We have a particular emphasis on translational(bench to clinic) research on IBD.
· Research on the intestinal epithelium to develop regenerative medicine approaches for IBD· The study of mucosal immunology to develop novel approaches for the diagnosis and treatment of IBD(3)

Education

We share our expertise and teaching program in graduate course education with the Department of Gastroenterology and Hepatology at TMDU. We are also involved in many programs designed for undergraduates.

(4)

Lectures & Courses

Our goal is to create future leaders who are able to reach the highest level of quality in IBD research throughthe training of fellows and graduate/undergraduate students.

(5)

Clinical Services & Other Works

We focus on developing highly advanced technologies, including novel procedures, for diagnosis and treatmentof IBD in collaboration with the Department of Gastroenterology and Hepatology at TMDU. In addition, we
have been playing a major role in nation-wide survey and multi-center studies on IBD, which is funded by theJapanese Ministry of Health, Labor and Welfare.

― 528 ―

Endowed Departments

Endowed Departments(6)

Clinical Performances· Development of new treatment protocol for IBD patients with stem cell therapy or immunomodulators.
· Development of minimally-invasive diagnostic modalities for inﬂammatory bowel diseases (i.e. MRE).
· Diagnosis and treatment of small intestinal lesions of inﬂammatory bowel diseases by double-balloon enteroscopy.
(7)

Publications[Original Articles]
1. Kengo Nozaki, Wakana Mochizuki, Yuka Matsumoto, Taichi Matsumoto, Masayoshi Fukuda, Tomohiro
Mizutani, Mamoru Watanabe, Tetsuya Nakamura. Co-culture with intestinal epithelial organoids allows
eﬃcient expansion and motility analysis of intraepithelial lymphocytes. J. Gastroenterol. 2016.03; 51(3);206-213
2. Shunsuke Komoto, Satoshi Motoya, Yuji Nishiwaki, Toshiyuki Matsui, Reiko Kunisaki, Katsuyoshi Matsuoka, Naoki Yoshimura, Takashi Kagaya, Makoto Naganuma, Nobuyuki Hida, Mamoru Watanabe, Toshifumi Hibi, Yasuo Suzuki, Soichiro Miura, Ryota Hokari, . Pregnancy outcome in women with inﬂammatory
bowel disease treated with anti-tumor necrosis factor and/or thiopurine therapy: a multicenter study fromJapan. Intest Res. 2016.04; 14(2); 139-145
3. Kazuo Ohtsuka, Kento Takenaka, Yoshio Kitazume, Toshimitsu Fujii, Katsuyoshi Matsuoka, Maiko
Kimura, Takashi Nagaishi, Mamoru Watanabe. Magnetic resonance enterography for the evaluationof the deep small intestine in Crohn’s disease. Intest Res. 2016.04; 14(2); 120-126
4. Ryohei Hayashi, Kiichiro Tsuchiya, Keita Fukushima, Nobukatsu Horita, Shuji Hibiya, Keisuke Kitagaki,
Mariko Negi, Eisaku Itoh, Takumi Akashi, Yoshinobu Eishi, Eriko Okada, Akihiro Araki, Kazuo Ohtsuka,
Shinji Fukuda, Hiroshi Ohno, Ryuichi Okamoto, Tetsuya Nakamura, Shinji Tanaka, Kazuaki Chayama,
Mamoru Watanabe. Reduced Human α-defensin 6 in Noninﬂamed Jejunal Tissue of Patients with Crohn’s
Disease. Inﬂamm. Bowel Dis. 2016.05; 22(5); 1119-1128
5. Taichi Matsumoto, Wakana Mochizuki, Yoichi Nibe, Shintaro Akiyama, Yuka Matsumoto, Kengo Nozaki,
Masayoshi Fukuda, Ayumi Hayashi, Tomohiro Mizutani, Shigeru Oshima, Mamoru Watanabe, Tetsuya
Nakamura. Retinol Promotes In Vitro Growth of Proximal Colon Organoids through a Retinoic AcidIndependent Mechanism. PLoS ONE. 2016.08; 11(8); e0162049
6. Shuji Hibiya, Kiichiro Tsuchiya, Ryohei Hayashi, Keita Fukushima, Nobukatsu Horita, Sho Watanabe,Tomoaki Shirasaki, Ryu Nishimura, Natsuko Kimura, Tatsunori Nishimura, Noriko Gotoh, Shigeru Oshima, Ryuichi Okamoto, Tetsuya Nakamura, Mamoru Watanabe. Long-term inﬂammation transforms
intestinal epithelial cells of colonic organoids. J Crohns Colitis. 2016.10;
7. Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Toru Nakata, Tatsuro Murano, Go Ito,
Hiromichi Shimizu, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Akihiro
Araki, Kazuo Ohtsuka, Ryuichi Okamoto, Mamoru Watanabe. PGE2 is a direct and robust mediator ofanion/ﬂuid secretion by human intestinal epithelial cells. Sci Rep. 2016.11; 6; 36795
8. Masanori Kobayashi, Shigeru Oshima, Chiaki Maeyashiki, Yoichi Nibe, Kana Otsubo, Yu Matsuzawa,
Yasuhiro Nemoto, Takashi Nagaishi, Ryuichi Okamoto, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru
Watanabe. The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonicdevelopment. Sci Rep. 2016.11; 6; 36780
9. Kento Takenaka, Kazuo Ohtsuka, Yoshio Kitazume, Katsuyoshi Matsuoka, Toshimitsu Fujii, Masakazu
Nagahori, Maiko Kimura, Tomoyuki Fujioka, Akihiro Araki, Mamoru Watanabe. Magnetic resonanceevaluation for small bowel strictures in Crohn’s disease: comparison with balloon enteroscopy. [ Epubahead of print] J. Gastroenterol. 2016.11;[Conference Activities & Talks]
1. K. Matsuoka, E. Saito, T. Fujii, K. Takenaka, F. Iwamoto, M. Kimura, M. Nagahori, K. Ohtsuka, M.
Watanabe. Eﬃcacy and safety of sequential rescue therapy with inﬂiximab and tacrolimus in patients― 529 ―

Endowed Departments

Endowed Departmentswith corticosteroid-refractory Ulcerative Colitis. 11th Congress of ECCO - European Crohn s and Colitis
Organisation 2016.03.19 Amsterdam（Nederland）
2. Takenaka K, Ohtsuka K, Kitazume Y, Nagahori M, Matsuoka K, Fujii T, Kimura M, Fujioka T, Watanabe
M. Enteroscopic and MR ﬁndings of small intestine in Crohn s disease. The 7th APTC 2016.04.20 Tokyo（JAPAN）
3. Tetsuya Nakamura. 【Session 2: Gut homeostasis and IBD】Intestinal stem cell transplantation andregenerative medicine. The 102nd JSGE-The 5th International Forum 2016.04.21 Tokyo（JAPAN）
4. Shuji Hibiya, Kiichiro Tsuchiya, Tomoaki Shirasaki, Keita Fukushima, Shigeru Oshima, Ryuichi Okamoto,Tetsuya Nakamura, Mamoru Watanabe. Construction of in vitro model for inﬂammatory bowel diseaseusing primary colonic organoid. DDW2016 2016.05.21 San Diego（USA）
5. Kohei Suzuki, Satoru Fujii, Ami Kawamoto, Fumiaki Ishibashi, Toru Nakata, Go Ito, Hiromichi Shimizu,
Kiichiro Tsuchiya, Tetsuya Nakamura, Kazuo Otsuka, Ryuichi Okamoto, Mamoru Watanabe. Singlecell level analysis reveals hoterogeneous expression of stem-cell speciﬁc genes in human small intestinalorganoids. DDW2016 2016.05.21 San Diego（USA）
6. Taichi Matsumoto, Wakana Mochizuki, Yoichi Nibe, Shintaro Akiyama, Yuka Matsumoto, Kengo Nozaki,
Tomohiro Mizutani, Shigeru Ochima, Mamotu Watanabe, Tetsuya Nakamura. Rethinol stimulates proliferation of epithelial stem cells of the proximal colon through a retinoic acid-indeoendent mechanism.
DDW2016 2016.05.21 San Diego（USA）
7. Toru Nakata, Go Ito, Hiromichi Shimizu, Satoru Fujii, Kohei Suzuki, Fumiaki Ishibashi, Ami Kawamoto,Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Ryuichi Okamoto, Mamoru Watanabe. Jagged1
is indispensable for the maintenance of tumor stem cell niche in APC-Deﬁcient intestinal adenomas.
DDW2016 2016.05.22 San Diego（USA）
8. Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Toru Nakata, Go Ito, Hiromichi Shimizu,
Tomohiro Mizutani, Kiichiro Tsuchiya, Tetsuya Nakamura, Kazuo Ohtsuka, Ryuichi Okamoto, Mamoru
Watanabe. In Vitro organoid model reveals PGF2 as a Robust Mediator of chloride & water secretion bt
human intestinal epithelial cells. DDW2016 2016.05.23 San Diego（USA）
9. Kazuo Ohtsuka, Kento Takenaka, Toshimitsu Fujii, Katsuyoshi Matsuoka, Masakazu Nagahori, Kengo
Nozaki, Masayoshi Fukuda, Mamoru Watanabe. Mucosal Healing is imoietant for better outcomes ofendosdopic balloon dilatation for smakk intestinal stricture of crohn’s disease. DDW2016 2016.05.23 San
Diego（USA）
10. Tetsuya Nakamura. 【Innovative Approaches to Curing Intestinal Failure: The Future of Cell and Tissue
Therapy】Therapeutic Transplantation of Human Intestinal Stem Cells. DDW2016 2016.05.23 San Diego（USA）
11. Katsuyoshi Matsuoka, Makoto Naganuma, Toshiyuki Matsui, Makoto Arai, Satoshi Tanida, Kazuya Kitamura, Noriyuki Horiki, Mikihiro Fujiya, Masaki Iimuro, Keiichi Mitsuyama, Masayuki Saruta, Takayuki
Matsumoto, Hiroko Nebiki, Motohiro Esaki, Fukunori Kinjo, Kiyoshi Oketani, Seiichiro Hojo, Hideto
Akama, Toshio Imai, Hirohito Tsubouchi, Toshifumi Hibi, Takanori Kanai. Safety, Tolerability and Eﬃcacy of E6011, Anti-Human Fractalkine Monoclonal Antibody, in the First-in-Patient Study for Crohn’s
Disease. DDW2016 2016.05.23 San Diego（USA）
12. Shinta Mizuno, Kosaku Nanki, Katsuyoshi Matsuoka, Keiko Ono, Makoto Mutaguchi, Mari Arai, Shinya
Sugimoto, Hiroki Kiyohara, Kozue Takeshita, Makoto Naganuma, Takanori Kanai. Eﬃcacy and safety of
fecal microbiota transplantation on ulcerative colitis. AOCC2016 2016.07.07 Kyoto International Conference Center, Kyoto（JAPAN）
13. Meng-Tzu Weng, Katsuyoshi Matsuoka, Mamoru Watanabe, JY Lee, Sang Hyoung Park, Suk-Kyun Yang,
Shu-Chen Wei. Thromboembolic events associated with inﬂammatory bowel disease in Asia: incidence
and possible risk factors analysis. AOCC2016 2016.07.07 Kyoto International Conference Center, Kyoto（JAPAN）
14. Tetsuya Nakamura. Stem-Cell Based Therapies for Intestinal Diseases. AOCC 2016 2016.07.08 Kyoto
International Conference Center, Kyoto（JAPAN）― 530 ―

Endowed Departments

Endowed Departments

15. Katsuyoshi Matsuoka, Naoki Yoshimura, Mamoru Watanabe, Satoshi Motoya, Keiichi Tominaga, Ryuichi
Iwakiri, Kenji Watanabe, Toshifumi Hibi, For the AJM300 Study Group. AJM300, an oral α 4 integrin
antagonist, as induction therapy for active ulcerative colitis: a randomized trial. AOCC2016 2016.07.08
Kyoto International Conference Center, Kyoto（JAPAN）
16. Tomoaki Shirasaki, Kiichiro Tsuchiya, Shuji Hibiya, Ryu Nishimura, Keita Fukushima, Sho Watanabe,
Ryuichi Okamoto, Tetsuya Nakamura and Mamoru Watanabe. Stimulation with the ligands of Toll likereceptors in primary human organoids. AOCC2016 2016.07.08 Kyoto International Conference Center,
Kyoto（JAPAN）
17. Maiko Kimura, Katsuyoshi Matsuoka, Eiko Saito, Toshimitsu Fujii, Kento Takenaka, Masakazu Nagahori, Kazuo Ohtsuka, Mamoru Watanabe. Salvage therapy for corticosteroid-refractory ulcerative colitis
patients. AOCC2016 2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）
18. Kento Takenaka, Kazuo Ohtsuka, Yoshio Kitazume, Masakazu Nagahori, Katsuyoshi Matsuoka, Toshimitsu Fujii, Maiko Kimura, Tomoyuki Fujioka, Mamoru Watanabe. Enteroscopic and MR ﬁndings of small
bowel in Crohn s disease. AOCC2016 2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）
19. Minami Hama, Ayumi Hayashi, Tomohiro Mizutani, Satoru Fujii, Tatsuro Murano, Kiichiro Tsuchiya, Tetsuya Nakamura, Ken Watanabe, Norio Shimizu, Ryuichi Okamoto, Mamoru Watanabe. Prevalence of viral
DNA in the mucosal tissue and cultured organoids of inﬂammatory bowel disease patients.. AOCC2016
2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）
20. Shuji Hibiya, Kiichiro Tsuchiya, Tomoaki Shirasaki, Keita Fukushima, Shigeru Oshima, Ryuichi Okamoto,Tetsuya Nakamura, Mamoru Watanabe. Mouse colonic organoid acquires wnt-independent survival property after long-term inﬂammation. AOCC2016 2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）
21. Toshimitsu Fujii, Kento Takenaka, Maiko Kimura, Katsuyoshi Matsuoka, Masakazu Nagahori, Kazuo
Ohtsuka, Mamoru Watanabe. The evaluation for small bowel strictures on MR enterocolonography inpatients with Crohn s disease. AOCC2016 2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）
22. Kiichiro Tsuchiya, Shuji Hibiya, Keita Fukushima, Tomoaki Shirasaki, Shigeru Oshima, Ryuichi Okamoto,Tetsuya Nakamura, Mamoru Watanabe. Atoh1 protein stabilization in colon tumor acquires the phenotype
of signet ring cell carcinoma with cancer stem cell enrichment. AOCC2016 2016.07.08 Kyoto International
Conference Center, Kyoto（JAPAN）
23. Kazuo Ohtsuka, Kento Takenaka, Toshimitsu Fujii, Katsuyoshi Matsuoka, Masakazu Nagahori, Maiko
Kimura, Masayoshi Fukuda, Nobuhiro Katsukura, Mamoru Watanabe. Mucosal healing improves prognosis of endoscopic balloon dilatation for small intestinal strictures of Crohn s disease. AOCC2016
2016.07.09 Kyoto International Conference Center, Kyoto（JAPAN）
24. Tetsuya Nakamura. Therapeutic transplantation of intestinal stem cells. International Conference on
Bio-fabrication and Bio-monitoring (ICBB 2016) 2016.10.14 National Taiwan University, Taipei（China）
25. Toshimitsu Fujii, Kento Takenaka, Maiko Kimura, Katsuyoshi Matsuoka, Masakazu Nagahori, Kazuo
Ohtsuka, Mamoru Watanabe. MR enterocolonography evaluation for small bowel strictures in patientswith Crohn s disease: comparison with balloon enteroscopy. UEGW2016 2016.10.18 Vienna（Austria）
26. Hibiya S, Tsuchiya K, Watanabe S, Shirasaki T, Oshima S, Okamoto R, Nakamura T, Watanabe M.
Construction of in vitro model of ulcerative colitis using mouse primary colonic organoid. UEGW2016
2016.10.18 Vienna（Austria）
27. Tsuchiya K, Hibiya S, Fukushima K, Shirasaki T, Oshima S, Okamoto R, Nakamura T, Watanabe M.
Atoh1 protein stabilization in colon tumor acquires the morphological change to signet ring cell carcinoma with cancer stem cell enrichment. APDW2016 2016.11.03 Kobe convention center, KOBE（JAPAN）
28. Fujii T, Takenaka K, Kitazume Y, Kimura M, Matsuoka K, Nagahori M, Ohtsuka K, Watanabe M. MR
enterocolonography can detect small bowel strictures and predict surgery in patients with Crohn s disease:
Comparison with balloon enteroscopy. APDW2016 2016.11.03 Kobe convention center, KOBE（JAPAN）
29. Tetsuya Nakamura. Stem cell-based therapies for intestinal diseases. International Conference on Recent
Advances in Inﬂammation 2016 2016.11.11 National Cheng Kung University, Tainan（Taiwan）― 531 ―

Endowed Departments

Endowed Departments

Department of Sleep Modulatory Medicine

Professor: Naohiko Inase
Associate Professor: Meiyo Tamaoka
Assistant Professor: Mizue Hobo
Assistant Professor: Toshihide Fujie
Technician: Megumi Sato(1)

Research

1.Eﬀects of NMDA-type glutamate receptor co-agonist on gamma oscillations and sleep in schizophrenia.
2.Open-label trial of hyperbaric oxygen therapy on sleep quality
3.Development of the evaluation system for the eﬃcacy of oral appliances on obstructive sleep apnea syndrome.
4.Development of portable home polysomnograph system.
5.The association between OSA and short term blood-pressure variability.
6.The association between OSA and interstitial pneumonia.
7. 24 hours SpO2 monitoring for patients with chronic respiratory failure(2)

Education

Education of sleep medicine for students, residents and technicians(3)

Clinical Services & Other Works

Clinical Center for Pleasant Sleep provides a variety of medical service for sleep disorder especially for sleep
apnea syndrome.
· Out-patient Clinic
Monday: AM Dr. Terada (Pulmonary Medicine)
PM Dr. Fujie (Pulmonary Medicine)
Tuesday: AM Dr. Hirai (Psychiatry)
Wednesday: AM Dr. Miyazaki (Health Service Center)
Thursday: AM Dr. Tamaoka (Sleep Modulatory Medicine)
PM Dr. Tamaoka (Sleep Modulatory Medicine)
Friday: AM Dr. Uezato (Psychiatry)
AM Dr. Tateishi (Pulmonary Medicine)
PM Dr. Tateishi(Pulmonary Medicine)· Outpatient visits: 5,282 (New:311) for FY 2016
· Examination: polysomnography: 134 patiensts for FY 2016

― 532 ―

Endowed Departments

Endowed Departments(4)

Publications[Original Articles]
1. Chiba S, Tsuchiya K, Nukui Y, Sema M, Tamaoka M, Sumi Y, Miyazaki Y, Inase N.. Interstitial Changes
in Asthma-COPD Overlap Syndrome (ACOS) The Clinical Respiratory Journal. 2016.02;
2. Hobo M, Uezato A, Nishiyama M, Suzuki M, Kurata J, Makita K, Yamamoto N, Nishikawa T. A case of
malignant catatonia with idiopathic pulmonary arterial hypertension treated by electroconvulsive therapy
BMC Psychiatry. 2016.05; 16; 130[Conference Activities & Talks]
1. Meiyo Tamoaka, et al.. CPAP treatment for SAS in multiple system atrophy. The 56th Annual Meetingof Japanese Respiratory Society 2016.04.08 Kyoto
2. Hiroyuki Ishiyama, Shusuke Inukai, Akira Nishiyama, Masayuki Hideshima, Shuhei Nakamura, Chisato
Ida, Yuko Mitsuma, Shota Hayashi, Akihito Uesato, Toshihide Fujie, Meiyo Tamaoka, Yasunari Miyazaki,
Masahiko Shimada, Noriyuki Wakabayashi . Eﬀect of jaw-opening exercise on prevention of temporomandibular disorders pain associated with oral appliance therapy and compliance in obstructive sleep
apnea patients: A randomized, double-blind, placebo-controlled trial.. Japanese Society of Sleep Researchthe 41th Annual Meeting 2016.07.07 Tokyo
3. Meiyo Tamaoka. Current status of CPAP therapy for OSAS in late stage elderly at our hospital. The
41st annual meeting of Japanese Society of Sleep Research 2016.07.07 Tokyo
4. Akihito Uezato, Meiyo Tamaoka. Evaluation of Sleep Apnea Syndrome using a portable electroencephalograph.. The 8th Congress of Integrated Sleep Medicine Society Japan 2016.08.06― 533 ―

Endowed Departments

Endowed Departments

Department of Women’s HealthProfessor Masakazu Terauchi MD PhD; Assistant Professor Asuka Hirose MD PhD (concurrent)(1)

OutlineJapanese women boast world s #1 longevity, although the ﬁnal stage of their lives is not necessarily of good
health-related quality. To stay physically and psychologically sound in later life, women need to optimize
their health starting from their midlife, especially through good diet and exercise. Tokyo Medical and Dental
University (TMDU) Department of Obstetrics and Gynecology have promoted midlife women s health with ourrenowned Systemic Health and Nutrition Education Program (SHNEP) since 1995, which inspired Kikkoman
Corporation to generously support to establish a new department in TMDU focusing on Health Maintenanceof Women through Food and Nutrition in 2012. Dr. Masakazu Terauchi, Associate Professor and Chairof TMDU Department of Women s Health, is intensively studying with his colleagues about the changes inwomen s bodies and minds induced by aging, and the eﬀects of bioactive food ingredients on them.
(2)

Research

Department of Women s Health has dealt with a variety of topics listed below since its inception in 2012,
mainly focusing our research on the eﬀects of bioactive food ingredients on women s physical and psychologicalhealth.
- Eﬀects of grape seed extract on middle-aged women’s health-related quality of life
- Eﬀects of hormone therapy and keishibukuryogan on blood pressure in perimenopausal and postmenopausal
women- Eﬀects of nonbenzodiazepine, melatonin receptor agonist, and Kampo medication on sleep disturbances inperimenopausal and postmenopausal women- Eﬀects of selective sertonin reuptake inhibitors on subjective and objective sleep parameters in middle-aged
women with depression- Eﬀects of oral contraceptive pills on sleep disturbances in young women with primary dysmenorrhea- Eﬀects of tomato juice on cardiovascular risk markers in middle-aged women- Eﬀects of soy isoﬂavone aglicone on middle-aged women’s health-related quality of life
- Menopausal hormone therapy: route of administration and platelet-derived microparticles- Eﬀects of soy lecithin on middle-aged women’s tiredness- Eﬀects of soy milk on middle-aged women’s sleep(3)

Education

Cooperating with the Department of Obstetrics and Gynecology, we have shared responsibility in the educationof Obstetrics and Gynecology, as well as in the training of medical students on clinical clerkship.
(4)

Clinical Services & Other Works

Cooperating with the Department of Obstetrics and Gynecology, we have provided a comprehensive diagnosis,
treatment, and disease management solution for women suﬀering from:- menopausal symptoms― 534 ―

Endowed Departments

Endowed Departments- premature ovarian insuﬃciency- postmenopausal osteoporosis- dyslipidemia- hypertension- pelvic organ prolapse- lower urinary tract syndrome- depression- anxiety disorder- insomnia- dysmenorrhea- premenstrual syndrome etc.
(5)

Publications[Original Articles]
1. Asuka Hirose, Masakazu Terauchi, Mihoko Akiyoshi, Yoko Owa, Kiyoko Kato, Toshiro Kubota. Depressive symptoms are associated with oxidative stress in middle-aged women: a cross-sectional study.
Biopsychosoc Med. 2016; 10; 12
2. Asuka Hirose, Masakazu Terauchi, Mihoko Akiyoshi, Yoko Owa, Kiyoko Kato, Toshiro Kubota. Low-dose
isoﬂavone aglycone alleviates psychological symptoms of menopause in Japanese women: a randomized,
double-blind, placebo-controlled study. Arch. Gynecol. Obstet.. 2016.03; 293(3); 609-615
3. A Hirose, M Terauchi, M Akiyoshi, Y Owa, K Kato, T Kubota. Subjective insomnia is associated withlow sleep eﬃciency and fatigue in middle-aged women. Climacteric. 2016.08; 19(4); 369-374[Misc]
1. Masakazu Terauchi. Menopausal symptoms and the Kampo medicine: Tokishakuyakusan, Kamishoyosan,
and Keishibukuryogan Recent Progress in Kampo Medicine in Obstetrics and Gynecology. 2016.04; 33;
23-30[Conference Activities & Talks]
1. Masakazu Terauchi. Palpitation in middle-aged women is associated with anxiety. 68th Annual Congressof Japan Society of Obstetrics and Gynecology 2016.04.22
2. Masakazu Terauchi, Asuka Hirose, Mihoko Akiyoshi, Toshiro Kubota. Palpitation in Middle-aged WomenIs Associated with Anxiety. 18th International Society of Psychosomatic Obstetrics and Gynecology
Congress 2016.05.12 Malaga, Spain
3. Masakazu Terauchi, Asuka Hirose, Mihoko Akiyoshi, Makoto Iizuka, Naoyuki Miyasaka, Toshiro Kubota.
Palpitation in middle-aged women is associated with anxiety. 15th World Congress on the Menopause
2016.09.30 Prague, Czech Republic― 535 ―

Endowed Departments

Endowed Departments

Department of Advanced Surgical Technology Research and Development

Associate Professor Katsuhiro OHUCHI, Ph.D
Associate Professor Tomohiro MIZUNO, MD, Ph.D
Part-time Lecturer Naoyuki YOKOYAMA, Ph.D
Part-time Lecturer Tarou KIMURA, Ph.D
Part-time Lecturer Daisuke SAKOTA, Ph.D
Part-time Lecturer Nobuo WATANABE, Ph.D
(1)

OutlineSurgical technology has been improving follows to the development of advanced instruments and therapeuticstrategies. On the one hand, innovation in surgery inevitably requires individual operator’s skill and tailoringof the intervention to the patient. The aim of our laboratory is research and development of developmental
pathway for modern surgical technology through the large animal experiment. Following to the framework forthe stages in surgical innovation, such as ideas, development, exploration, assessment and pre-clinical study,
we will serve as a mediator between basic science and clinical practice. And more, implementation of humanresources development in the ﬁeld of advanced surgery is also another intention of our laboratory.
(2)

Publications[Original Articles]
1. Fujiwara T, Sakota D, Ouchi K, Endo S, Tahara T, Murashige T, Kosaka R, Oi K, Mizuno T, Maruyama
O, Arai H.. Optical dynamic analysis of thrombus inside a centrifugal blood pump during extracorporeal
mechanical circulatory support in a porcine model Artif Organs. 2016; In Press;
2. Daisuke Sakota, Tomotaka Murashige, Ryo Kosaka, Tatsuki Fujiwara, Katsuhiro Ouchi, Masahiro Nishida,
Osamu Maruyama. Noninvasive optical imaging of thrombus formation in mechanical circulatory support
devices Journal of Biorheology. 2016; 30; 6-12
3. Daisuke Sakota, Tatsuki Fujiwara, Katsuhiro Ouchi, Katsuyuki Kuwana, Hiroyuki Yamazaki, Osamu
Maruyama. Development of an Optical Detector of Thrombus Formation on the Pivot Bearing of a
Rotary Blood Pump. Artiﬁcial Organs. 2016.09; 40(9); 834-841[Conference Activities & Talks]
1. K Ohuchi, T Mizuno, K Oi, M Yashima, T Hachimaru, E Nagaoka, T Fujiwara, H Kuroki, D Tasaki, M
Takeshita, R Kinoshita, S Endo, M Kanai, S Suzuki, T Kimura, H Arai. Attempt for the Developmentof Cardiac Surgery Training Program Combined with Live Animal Surgery. The 63rd Annual Meeting ofJapanese Association for Animal Science 2016.05.18
2. Ohuchi K, Mizuno T, Oi K, Yashima M, Hachimaru T, Nagaoka E, Fujiwara T, Kuroki H, Tasaki D,
Takeshita M, Kinoshita R, Endo S, Kanai M, Kimura T, Arai H. Development of Heart Surgery Training
Program Combined with Live Animal Surgery. 24th Congress of the International Society for Rotary
Blood Pumps 2016.09.21
3. Fujiwara T, Arai H, Mizuno T, Oi K, Yashima M, Hachimaru T, Nagaoka E, Kuroki H, Tasaki D,
Takeshita M, Kinoshita R, Ohuchi K. Which is a better acute-phase circulatory support for cardiogenic― 536 ―

Endowed Departments

Endowed Departments

shock patients in INTERMACS Proﬁle-1: A pulsatile VAD or a centrifugal pump?. 24th Congress of theInternational Society for Rotary Blood Pumps 2016.09.21
4. Sakota D, Fujiwara T, Ouchi K, Kuwana K, Yamazaki H, Arai H, Maruyama O. Development of an opticalthrombus detector on the pivot bearing of a rotary blood pump. 24th Congress of the International Society
for Rotary Blood Pumps 2016.09.22― 537 ―

Endowed Departments

Endowed Departments

Department of Arteriosclerosis and Vascular Biology

Associate Professor Shohei Shinozaki
Assistant Professor Yasuko Abe(1)

OutlineThe overall focus of our laboratory is to clarify the mechanism of the arteriosclerosis clear as well as aiming
at development of the novel remedy of the arteriosclerosis. We also try to understand the role of nitrogenoxide in the molecular pathogenesis in human diseases. Inﬂammation is implicated in a variety of humandiseases, whereas it is a necessary and adaptive response to environmental or intrinsic stress. We found that
S-nitrosylation (the covalent attachment of nitric oxide to thiols) plays the important role in insulin resistanceand aging related diseases. We’re analyzing these mechanisms using the technique of the biochemistry, themolecular biology, proteomic approach and genetic alteration mouse, such as S-nitrosoglutathione reductase(GSNOR) deﬁciency or transgene.
(2)

Research· Basic research on arteriosclerosis· Search and identiﬁcation for novel S-nitrosylated protein· Molecular mechanism of metabolic syndromes through S-nitrosylation(3)

Education

In accordance with aging and westernization of diet,atherosclerotic disease has been increasing in Japan. Recently, the chronic inﬂammation has been focused as the mechanism of arteriosclerosis. It has been demonstrated
that inﬂammation is deeply involved in the initial stage of atherosclerosis. However, treatments with anti- inﬂammatory drugs, such as NSAIDs, for atherosclerosis generally have not been successes. Thus we consider
that there are some unknown mechanisms between development of arteriosclerosis and inﬂammation.
(4)

Lectures & Courses

Our course’s education policy is to obtain basic research skills for elucidating pathogenesis of atherosclerosis.
(5)

Publications[Original Articles]
1. Sasaki Mari, Shinozaki Shohei, Shimokado Kentaro. Sulforaphane promotes murine hair growth by accelerating the degradation of dihydrotestosterone. Biochem Biophys Res Commun. 2016.03; 472(1); 250-254
2. Chiba Tsuyoshi, Noji Keiko, Shinozaki Shohei, Suzuki Sachina, Umegaki Keizo, Shimokado Kentaro. Dietinduced non-alcoholic fatty liver disease aﬀects expression of major cytochrome P450 genes in a mousemodel. J Pharm Pharmacol. 2016.12; 68(12); 1567-1576― 538 ―

Endowed Departments

Endowed Departments[Conference Activities & Talks]

1. Shohei Shinozaki . S-nitrosylation of SIRT1 as a hub of and inﬂammatory spira. The 16th Annual
Scientiﬁc Meeting of the Nitric Oxide Society of Japan 2016.05.20 Sendai
2. Shohei Shinozaki. Type 2 diabetes and chronic inﬂammation.. The 58th Annual meeting of Japan
Geriatrics Society 2016.06.09 Kanazawa[Others]
1. Grant-in-Aid for Young Scientists (B) JSPS KAKENHI (16K19048)
S-nitrosylation, a novel posttranslational protein modiﬁcation, mediated glycolipid metabolism disorderand chronic inﬂammation. 2016-2017
2. Takeda Science Foundation for Medical Research
The missing link between the metabolic syndrome pathogenesis and chronic inﬂammation. 2016-2018
3. Grant-in-Aid for Exploratory Research JSPS KAKENHI (16K15120)
Elucidation of a role of de-nitrosylation in septic myocardial injury and development for therapeutic drugs.
PI: Toshihiro Tanioka. Role on Project: Co- Investigator 2016-2017― 539 ―

Joint Research Departments+PJOU 3FTFBSDI %FQBSUNFOUT

Department of Translational Oncology< Department of Translational Oncology>
Associate Professor: Megumi ISHIGURO
Junior Associate Professor (concurrent): Toshiaki ISHIKAWA(1)

OutlineDepartment of Translational Oncology aims to establish the personalized therapy in chemotherapy for
gastrointestinal cancers through identiﬁcation of the predictive factors for chemo-responsiveness and prognosis.
(2)

Research

Main themes of our research activities is identiﬁcation of the predictive factors for chemo-responsiveness andprognosis by using our well-organized clinical database, fresh frozen and paraﬃn-embedded samples, and molecular biological technique, i.e. gene expression micro allay analysis, and DNA copy number analysis.
(3)

Publications[Original Articles]
1. Masuda T, Ishikawa T, Mogushi K, Okazaki S, Ishiguro M, Iida S, Mizushima H, Tanaka H, Uetake H,
Sugihara K.. Overexpression of the S100A2 protein as a prognostic marker for patients with stage II and
III colorectal cancer. Int J Oncol. 2016; 48(3); 975-982
2. Ishikawa T, Uetake H, Murotani K, Kobunai T, Ishiguro M, Matsi S, Sugihara K.. Genome-wide DNA
Copy-number Analysis in ACTS-CC Trial of Adjuvant Chemotherapy for Stage III Colonic Cancer. Anticancer Research. 2016; 36; 853-860
3. Baba H, Ishikawa T, Mogushi K, Ishiguro M, Uetake H, Tanaka H, Sugihara K.. Identiﬁcation of SATB1
as a speciﬁc biomarker for lymph node metastasis in colorectal cancer. Anticancer Research. 2016; 36;4069-4076[Conference Activities & Talks]
1. Kajiwara Y, Ishiguro M, Teramukai S, Matsuda C, Fujii S, Kinugasa Y, Nakamoto Y, Kotake M, Sakamoto
Y, Kurachi K, Maeda A, Komori K, Tomita N, Simada Y, Takahashi K, Kotake K, Watanabe M, Mochizuki
H, Sugihara K.. A randomized phase III trial of 1-year adjuvant chemotherapy with oral tegafur-uracil(UFT) vs. surgery alone in stage II colon cancer.. American Society of Clinical Oncology (ASCO) 2016
Annual Meeting 2016.06.04 Chicago (USA)
2. Miura T, Sunami E, Kusumoto T, Ota M, Yoshida K, Tomita N, Maeda A, Mochizuki I, Okabe M,
Kunieda K, Yamauchi J, Itabashi M, Kotake K, Takahashi K, Baba H, Boku N, Aiba K, Ishiguro M,
Morita S, Sugihara K.. Planned safety analysis of the ACTS-CC 02 trial: A randomized phase III trialof S-1/oxaliplatin (SOX) versus UFT/LV as adjuvant chemotherapy for high-risk stage III colon cancer..
American Society of Clinical Oncology (ASCO) 2016 Annual Meeting 2016.06.04 Chicago (USA)― 540 ―

Joint Research Departments+PJOU 3FTFBSDI %FQBSUNFOUT
3. Ishiguro M.. Colorectal cancer database in Japan..
2016.11.05 Seoul (Korea)― 541 ―

68th Congress of the Korean Surgical Society.

University Hospitals

Central Clinical Facilities

Clinical Laboratory

General Manager-Proﬀesor : Shuji Tohda
Associate Manager-Junior Associate Professor : Tadashi Kanouchi
Assistant Professor : Naomi Murakami
Assistant Professor : Ryoko Azuma
Assistant Professor : Miyako Murakawa
Medical Staﬀ : Shintaro Iida
(1)

Research

Our research subjects are1) New genetic tests for hematological disorders,2) Development of tests for molecular pathology and drug sensitivity of hematological malignancies,3) Morphological abnormalities of the cells in myelodysplastic syndrome and myeloproliferative disorders,
4) Genotypic analysis of bacteria for monitoring those transmission in the hospital,
5) Development of electrophysiological diagnostic tests for peripheral neuropathies,6) Clinical and electrophysiological study for amyotrophic lateral sclerosis,7) Quality control of nerve conduction study,8) Development of medical information system for clinical laboratory tests.

(2)

Education

The staﬀs lectured on clinical laboratory medicine and gave technical training on clinical laboratory tests andphysiolosical function tests to not only the medical students and medical technologist students in the facultyof medicine of the university but also those in the other vocational school for medical technologists. We gave
lessons to our university students in the master s course of graduate school of medical and dental sciences ,too. In our clinical laboratory in 2016, ten junior residents of the medical hospital of university had a generaltraining for clinical laboratory medicine, including bacteriological examinations and ultrasonography. Hands-on
seminars of Gram staining, urinary sediment, cardiac and abdominal ultrasonography have been repeatedly
held for residents of the hospital.
(3)

Clinical Services & Other Works

Clinical laboratory bears an important responsibility for advanced and high quality medical care. Our
clinical laboratory is based on the principle of providing the speedy and high quality tests. The highest levelof advanced tests, such as qualitative and quantitative analysis of various viral DNA by the PCR method, arealso introduced here. We starts taking a blood sample at 8:05 and it results in shortening the waiting time ofpatients and in speedy report of the test results. In the night time and holidays, the clinical laboratory provides
blood products for transfusion in cooperation with the blood transfusion service of the hospital. The updated― 542 ―

University Hospitals

Central Clinical Facilities

information on antibiotic sensitivity of the pathogens in each ward is also provided online regularly. Together
with the division of infection control and prevention, we monitor the nosocomial transmission of bacteria suchas MRSA by genotypic analysis of those. Our clinical laboratory and blood transfusion service have received
accreditation of ISO15189 (Medical laboratories - Particular requirements for quality and competence) in June
2014, and renewed it with the latest version in December 2016. It means that the clinical laboratory is aninternational standard on quality and that our hospital is allowed to conduct the international clinical trials.
(4)

Publications[Original Articles]
1. Mari Kamiya, Peter Y Shane, Makoto Soejima, Shuji Tohda, Nobuyuki Miyasaka, Hitoshi Kohsaka.
IgG4-Related Sialoadenitis with a Skin Lesion and Multiple Mononeuropathies Suggesting Coexistent
Cryoglobulinemic Vasculitis. Intern. Med.. 2016; 55(10); 1355-1361
2. Uchida Emi, Watanabe Ken, Oshikawa Gaku, Sakasita Chizuko, Kurosu Tetsuya, Fukuda Tetsuya, Arai
Ayako, Murakami Naoki, Miura Osamu, Yamamoto Masahide. Refractory primary myeloid sarcoma of
the breast with MLL-AF9 rearrangement. The Japanese Journal of Clinical Hematology. 2016.01; 57(1);3. Fukiko Kawai-Kitahata, Yasuhiro Asahina, Shinji Tanaka, Sei Kakinuma, Miyako Murakawa, Sayuri Nitta,
Takako Watanabe, Satoshi Otani, Miki Taniguchi, Fumio Goto, Hiroko Nagata, Shun Kaneko, Megumi
Tasaka-Fujita, Yuki Nishimura-Sakurai, Seishin Azuma, Yasuhiro Itsui, Mina Nakagawa, Minoru Tanabe,
Shinichi Takano, Mitsuharu Fukasawa, Minoru Sakamoto, Shinya Maekawa, Nobuyuki Enomoto, Mamoru
Watanabe. Comprehensive analyses of mutations and hepatitis B virus integration in hepatocellular
carcinoma with clinicopathological features. J. Gastroenterol. 2016.03; 51(5); 473-486
4. Yokota T, Kanouchi T, Ohkubo T. ALS: Quantitative analysis of disease progression from a new point ofview 2015 Annual report of the research committee of CNS degenerative diseases, The ministry of health,labour and welfare of Japan. 2016.03; 58-61
5. Miki Taniguchi, Megumi Tasaka-Fujita, Mina Nakagawa, Takako Watanabe, Fukiko Kawai-Kitahata,
Satoshi Otani, Fumio Goto, Hiroko Nagata, Shun Kaneko, Sayuri Nitta, Miyako Murakawa, Yuki NishimuraSakurai, Seishin Azuma, Yasuhiro Itsui, Kenichi Mori, Shintaro Yagi, Sei Kakinuma, Yasuhiro Asahina,
Mamoru Watanabe. Evaluation of Interferon Resistance in Newly Established Genotype 1b Hepatitis C
Virus Cell Culture System. J Clin Transl Hepatol. 2016.03; 4(1); 5-11
6. Seishin Azuma, Yasuhiro Asahina, Yuki Nishimura-Sakurai, Sei Kakinuma, Shun Kaneko, Hiroko Nagata,
Fumio Goto, Satoshi Ootani, Fukiko Kawai-Kitahata, Miki Taniguchi, Miyako Murakawa, Takako Watanabe, Megumi Tasaka-Fujita, Yasuhiro Itsui, Mina Nakagawa, Mamoru Watanabe. Eﬃcacy of additional
radiofrequency ablation after transcatheter arterial chemoembolization for intermediate hepatocellular
carcinoma. Hepatol Res. 2016.04; 46(4); 312-319
7. Megumi Akiyama, Kota Yoshifuji, Tetsuya Fukuda, Shuji Tohda, Tohru Miki, Osamu Miura, Masahide
Yamamoto. Fulminant visceral disseminated varicella-zoster virus infection without skin involvement ina patient with autoimmune hemolytic anemia on prednisolone therapy. Rinsho Ketsueki. 2016.04; 57(4);467-471
8. Noriko Kawaguchi-Ihara, Mai Itoh, Ikuo Murohashi, Shuji Tohda. Establishment of a quenching probe
method for detection of NPM1 mutations in acute myeloid leukemia cells. Oncol Lett. 2016.04; 11(4);2429-2432
9. Shun Kaneko, Sei Kakinuma, Yasuhiro Asahina, Akihide Kamiya, Masato Miyoshi, Tomoyuki Tsunoda,
Sayuri Nitta, Yu Asano, Hiroko Nagata, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako Murakawa, Yasuhiro Itsui, Mina Nakagawa, Seishin Azuma, Hiromitsu Nakauchi, Hironori Nishitsuji, Saneyuki Ujino,
Kunitada Shimotohno, Masashi Iwamoto, Koichi Watashi, Takaji Wakita, Mamoru Watanabe. Human
induced pluripotent stem cell-derived hepatic cell lines as a new model for host interaction with hepatitis
B virus. Sci Rep. 2016.07; 6; 29358
10. Hiroko Nagata, Mina Nakagawa, Yuki Nishimura-Sakurai, Yu Asano, Tomoyuki Tsunoda, Masato Miyoshi,
Shun Kaneko, Fumio Goto, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako Murakawa, Sayuri Nitta,
Yasuhiro Itsui, Seishin Azuma, Sei Kakinuma, Naoko Tojo, Shuji Tohda, Yasuhiro Asahina, Mamoru― 543 ―

University Hospitals

Central Clinical Facilities

Watanabe, . Serial measurement of Wisteria ﬂoribunda agglutinin positive Mac-2-binding protein is
useful for predicting liver ﬁbrosis and the development of hepatocellular carcinoma in chronic hepatitis C
patients treated with IFN-based and IFN-free therapy.[ Epub ahead of print] Hepatol Int. 2016.07;
11. Fumio Goto, Sei Kakinuma, Masato Miyoshi, Tomoyuki Tsunoda, Shun Kaneko, Ayako Sato, Yu Asano,
Satoshi Otani, Seishin Azuma, Hiroko Nagata, Fukiko Kawai-Kitahata, Miyako Murakawa, Sayuri Nitta,
Yasuhiro Itsui, Mina Nakagawa, Yasuhiro Asahina, Mamoru Watanabe. Bone morphogenetic protein-4
modulates proliferation and terminal diﬀerentiation of fetal hepatic stem/progenitor cells. Hepatol. Res.
2016.09;
12. Shinji Ogihara, Ryoichi Saito, Etsuko Sawabe, Michio Hagihara, Shuji Tohda. First Japanese case of
infectious endocarditis due to Enterococcus faecalis small-colony variants. J. Infect. Chemother. 2016.10;
22(10); 716-719
13. Gene Igawa, Mika Casey, Etsuko Sawabe, Yoko Nukui, Shu Okugawa, Kyoji Moriya, Ryuji Koike, Shuji
Tohda, Ryoichi Saito. Comparison of agar dilution and broth microdilution methods for Clostridium
diﬃcile antimicrobial susceptibility testing. J Glob Antimicrob Resist. 2016.12; 43-45
14. Miyako Murakawa, Yasuhiro Asahina, Fukiko Kawai-Kitahata, Mina Nakagawa, Sayuri Nitta, Satoshi
Otani, Hiroko Nagata, Shun Kaneko, Yu Asano, Tomoyuki Tsunoda, Masato Miyoshi, Yasuhiro Itsui,
Seishin Azuma, Sei Kakinuma, Yasuhito Tanaka, Sayuki Iijima, Kaoru Tsuchiya, Namiki Izumi, Shuji
Tohda, Mamoru Watanabe. Hepatic IFNL4 expression is associated with non-response to interferonbased therapy through the regulation of basal interferon-stimulated gene expression in chronic hepatitis
C patients. [ Epub ahead of print] J. Med. Virol. 2016.12;[Conference Activities & Talks]
1. Amano E, Suzuki M, Ozaki K, Ishibashi S, Akaza M, Kanouchi T, Tohda S, Yokota T. A case of sensory
dominant CIDP with conduction delay predominantly in the most distal segment (Japanese). 10th Tokyo
Metropolitan Neuromuscular Electrodiagnosis Forum 2016.01.30 Tokyo
2. Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyoshi M, Asano Y, Nagata H,
Goto F, Otani S, Kitahata F, Murakawa M , Itsui Y, Nakagawa M, Azuma S, Watanabe M. A Model forInteraction between HBV and Host Cells Derived from Human Induced Pluripotent Stem Cells. The 25th
Asian Paciﬁc Association for the Study of the Liver 2016.02.22 International Convention Center Pamir,Tokyo（JAPAN）
3. Kawai-Kitahata F, Asahina Y, Tanaka S, Kakinuma S, Murakawa M, Nitta S, Otani S, Goto F, Nagata
H, Kaneko S, Asano Y, Miyoshi M, Tsunoda T, Azuma S, Itsui Y, Nakagawa M, Tanabe M, Maekawa
S, Enomoto N, Watanabe M. Comprehensive analyses of mutations and hepatitis B virus integrationin hepatocellular carcinoma. The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22
International Convention Center Pamir, Tokyo（JAPAN）
4. Nagata H, Sakurai Y, Nakagawa M, Kakinuma S, Azuma S, Murakawa M, Nitta S, Itsui Y, Asahina Y,
Watanabe M. Serial changes in M2BPGi levels as predictors of ﬁbrosis and HCC in chronic hepatitis C.
The 25th Asian Paciﬁc Association for the Study of the Liver 2016.02.22 International Convention Center
Pamir, Tokyo（JAPAN）
5. Shohei Nogami, Mai Itoh, Shuji Tohda. Clinical usefulness of allele-speciﬁc/quenching probe method for
detection of the MYD88 L265P mutation. The 14th Asian Society for Clinical Pathology and Laboratory
Medicine Congress 2016.03.26 Taipei
6. Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyoshi M, Asano Y, Nagata H ,Goto F, Otani S, Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Watanabe M. A new model
for studying interaction between hepatitis B virus and host cells derived from human induced pluripotent
stem cells. EASL The International Liver Congress 2016 2016.04.14 Barcelona（Spain）
7. Kanouchi T, Sekiguchi T, Yokota T. A pilot study on disease progression of ALS by quantitative analysis
from a new viewpoint. 57th annual meeting of the Japanese Society of Neurology 2016.05.18 Kobe
8. Ohta N, Kanouchi T, Akaza M, Aoyagi E, Narumi J, Yanagi N, Sumi Y, Yokota T, Hagihara M, Tohda
S. A study of quality control of nerve conduction study. 57th annual meeting of the Japanese Society of
Neurology 2016.05.20 Kobe

― 544 ―

University Hospitals

Central Clinical Facilities

9. Shintaku H, Kanouchi T, Yokota T. Pathophysiology of myoclonus in corticobasal syndrome. 57th annual
meeting of the Japanese Society of Neurology 2016.05.21 Kobe
10. Ohhara M, Sanjo N, Majima T, Kanouchi T, Yokota T. Long term prognosis of anti-MAG antibody
positive neuropathy. 57th Annual meeting of the Japanese Society of Neurology 2016.05.21 Kobe
11. Tadashi Kanouchi. Nerve conduction study in the upper extremity. 13th neuromuscular electrodiagnosis
seminar 2016.07.02 Tokyo
12. Kanouchi T, Higashihara K. Ulnar entrapment neuropathy. 13th neuromuscular electrodiagnosis seminar2016.07.02 Tokyo
13. Tadashi Kanouchi. First step in nerve conduction study. 11th technical training courses of clinicalneurophysiology, Tokyo 2016.07.31 Tokyo
14. Kazuaki Yamamoto, Seiya Watanabe, Michio Hagihara, Shuji Tohda. Development of a novel measurement method for L-hydroxyproline using a new enzyme. The 32th World Congress of Biomedical
Laboratory Science 2016.09.02
15. Naoya Ichimura, Ayako Itoi, Yuki Ohkubo, Yuki Koda, Michio Hagihara, Shuji Tohda. Development of
an internal quality control application for immature granulocytes diﬀerential. The 32th World Congressof Biomedical Laboratory Science 2016.09.02 Kobe
16. Naoya Ichimura, Ayako Itoi, Yuki Ohkubo, Yuki Koda, Michio Hagihara, Shuji Tohda. Evaluation of aninternal QC application for correctly recognizing immature granulocytes on blood smear. The 32th World
Congress of Biomedical Laboratory Science 2016.09.02 Kobe
17. Kanouchi T, Sekiguchi T, Tohda S, Yokota T. Does the impairment of upper motor neurons have aninﬂuence upon the speed of degeneration of lower motor neurons in ALS. 46th annual meeting of theJapanese Society of Clinical Neurophysiology 2016.10.28 Kohriyama
18. Iida S, Kanouchi T, Suzuki M, Sanjo N, Nishida Y, Yokota T. Distribution patterns of nerve conduction
abnormality in CIDP. 46th annual meeting of the Japanese Society of Clinical Neurophysiology 2016.10.28
Kohriyama
19. Aoyagi E, Kanouchi T, Akaza M, Iida S, Narumi J, Ohta N, Sumi Y, Yokota T, Hagihara M, Tohda S.
A study of quality control of nerve conduction study. 46th annual meeting of the Japanese Society ofClinical Neurophysiology 2016.10.29 Kohriyama
20. Shun Kaneko, Sei Kakinuma, Yasuhiro Asahina, Akihide Kamiya, Masato Miyoshi, Tomoyuki Tsunoda,
Sayuri Nitta, Ayako Sato, Yu Asano, Hiroko Nagata, Satoshi Otani, Fukiko Kawai-Kitahata, Miyako
Murakawa, Yasuhiro Itsui, Mina Nakagawa, Seishin Azuma, Mamoru Watanabe. Genetically modiﬁed
human induced pluripotent stem cell-derived hepatic progenitor-like cell lines as a model for interaction
between hepatitis B virus and host cells. AASLD The Liver meeting 2016 2016.11.11 Boston（USA）
21. Erika Shiratori, Mai Itoh, Mika Ohtaka, Shohei Nogami, Shuji Tohda. Mechanisms of suppressive eﬀects
of MYD88 Inhibitors on the Growth of Lymphoma and Leukemia Cells. The 58th American Society of
Hematology 2016.12.04 San Diego― 545 ―

University Hospitals

Central Clinical Facilities

Transfusion Medicine

Director(Lecturer) Michiko KAJIWARA
Assistant Director(Medical Technologist) Naoki OHTOMO
Section Chief Medical Technologist Keiko BABA
Assisitant Section Chief Medical Technologist Yukiko OHISHIClinical Fellow Shihoko SUWA
Medical Technologist Yukari USUIMedical Technologist Kaoru OKUYAMA
Medical Technologist Shiho KOBAYASHI
Medical Technologist Chihiro TOYAMA
Medical Technologist Eriko FURUYA(1)

Research

1) Practice of safe and appropriate transfusion therapy (including prevention of medical accident related transfusion)2) Basic and clinical research of hematopoietic stem cell transplantation(2)

Lectures & Courses

Transfusion therapy is a supplementation of the blood component, but it also has aspects of cell therapy andtransplantation. So, it is important to practice safe and appropriate transfusion therapy. Clinical tests oftransfusion, such as blood type test, are most basic immunological test technique. The accurate understanding
and practice of these tests is also necessary for the safety of medical treatment. From this point of view, we
educate the students of school of medicine, school of allied health sciences, graduate school of medical anddental sciences, medical doctors, and co-medicals.

(3)

Clinical Services & Other Works

4. Clinical Services (The result of 2016)
1) The amount of blood products used
Red cell component products 11,657 Units (5,892 bags)
Platelet concentration 25,749 Units (2,239 bags)
Fresh frozen plasma 9,880 Units (4,233 bags)
2) Autologous blood collection and transfusion
Autologous blood collection 363 cases (468 times，909 Units)
Autologous blood transfusion 317 cases (764 Units)
3) The number of clinical tests of transfusion
Blood typing 10,760
Anti red blood cell antibody test 5,298
Cross match 9,152
4) Hematopoetic stem cell harvest
Autologous peripheral blood stem cell harvest 6 cases 6 times― 546 ―

University Hospitals

Central Clinical Facilities
Allogenic peripheral blood stem cell harvest 5 cases 7 times
Autologous peripheral mononuclear cell harvest 0 case 0 time
Allogenic bone marrow harvest 13 cases 13 times(Including Japan Marrow Donor Program donors)5) Hematopoetic stem cell transplantation(The evaluation and preservation of the stem cells were done in our
department)
Autologous peripheral blood stem cell transplantation 7 cases 8 times
Allogenic peripheral blood stem cell transplantation 4 cases 4 times
Autologous peripheral mononuclear cell transplantation 0 case 0 time
Allogenic bone marrow transplantation 14 cases 14 times
Allogenic umbilical cord blood transplantation 4 cases 4 times(4)

Publications[Original Articles]
1. Yuki Aoki, Reiji Miyawaki, Kohsuke Imai, Masatoshi Takagi, Michiko Kajiwara, Yasuyoshi Ishiwata,
Masato Yasuhara, Tomohiro Morio, Shuki Mizutani, Daisuke Tomizawa. Haploidentical Bone Marrow
Transplantation With Clofarabine and Busulfan Conditioning for a Child With Multiple Recurrent Acute
Lymphoblastic Leukemia. J. Pediatr. Hematol. Oncol.. 2016.01; 38(1); 39-41
2. Takeshi Sato, Tsubasa Okano, Mari Tanaka-Kubota, Shunsuke Kimura, Satoshi Miyamoto, Shintaro Ono,
Motoi Yamashita, Noriko Mitsuiki, Masatoshi Takagi, Kohsuke Imai, Michiko Kajiwara, Takasuke Ebato,
Shohei Ogata, Hirotsugu Oda, Osamu Ohara, Hirokazu Kanegane, Tomohiro Morio. Novel compound
heterozygous mutations in a Japanese girl with Janus kinase 3 deﬁciency. Pediatr Int. 2016.09; 58(10);1076-1080
3. Daisuke Tomizawa, Akifumi Endo, Michiko Kajiwara, Hirotoshi Sakaguchi, Kimikazu Matsumoto, Makoto
Kaneda, Takashi Taga. Acute lymphoblastic leukemia in patients with Down syndrome with a previous
history of acute myeloid leukemia. Pediatr Blood Cancer. 2016.12;[Conference Activities & Talks]
1. Shiho KOBAYASHI, Naoki OTOMO, Kaoru OKUYAMA, Shihoko SUWA, Michiko KAJIWARA. Studyof the performance and usefulness of "ORTHO VISION Max" in TMDU. The 32nd World Congress ofBiomedical Laboratory Science 2016.09.02 Kobe International Conference Center, JAPAN2. Marie Scully, Paul Knoebl, MD, Karim Kentouche, Lawrence Rice, Jerzy Windyga, Reinhard Schneppenheim, Johanna A Kremer Hovinga, Michiko Kajiwara, Caterina Maggiore, Jennifer Doralt, Leah Martell,and Bruce M. Ewenstein. Pharmacodynamic Proﬁle of a Recombinant ADAMTS13 (BAX930) in Hereditary Thrombotic Thrombocytopenic Purpura (Upshaw-Schulman Syndrome (USS)). American Society of
Hematology, 58th Annual Meeting 2016.12.03 San Diego, CA, USA― 547 ―

University Hospitals

Central Clinical Facilities

Hyperbaric Medical Center

Senior Director and Associate Professor; Kazuyoshi YAGISHITA
Junior Associate Professor ; Mitsuhiro ENOMOTO
Senior Resident; Takuya OYAIZU
Adjunct Lecturer; Yasushi KOJIMA, Masaharu SHIBAYAMA
Researcher; Masaki HORIE, Toshihiro KONDOH, Naohiro MITSUMOTO
Staﬀ Assistant; Kiyomi ITOH

(1)OutlineHyperbaric oxygen therapy (HBO), which can dissolve oxygen in serum in population to atomic pressure andtransport oxygen to ischemic tissue, is an established therapy for treatment of several conditions, including
decompression illness, carbon monoxide poisoning, acute arterior disturbance, and peripheral ischemic disease.
The mechanism of HBO can be described as hyperoxygenation in ischemic soft tissues, reduction of edema,
stimulation of ﬁbroblast proliferation and diﬀerentiation, increased collagen formation and cross-linking, angiogenesis, and improved preservation of energy metabolism.
This curious treatment has clinically many kinds of eﬃcacy, however, the mechanism of the eﬀect has not been
fully understood, and many researchers in the world still attempt to reveal the mechanism of the eﬀect of HBO.
This HBO can stimulate the interest of medical students, basic researchers, and clinical doctors, and this hyperbaric medical center can provide opportunities to study hyperbaric oxygen therapy ﬁeld.

(2)

ResearchResearch Subjects
1) Soft tissue injuries related with sports activities
2) HBO for conditioning in sports activities
3) Diving medicine
4) Hyperbaric oxygen therapy(3)

Education

HBO can stimulate the interest of medical students, basic researchers, and clinical doctors, and this hyperbaric
medical center can provide opportunities to study hyperbaric oxygen therapy ﬁeld.

(4)

Clinical Services & Other Works

In 2015, 6,624 times hyperbaric oxygen therapy (HBO) in 685 patients were performed in the university hospital.

― 548 ―

University Hospitals

Central Clinical Facilities(5)

Clinical Performances

HBO is applied for several conditions, including decompression illness, carbon monoxide poisoning, infection,wound healing, delayed radiation injury, acute arterial disturbance, and peripheral ischemic disease. Recently,
for the purpose of rapid recovery from injury, we perform HBO aggressively for soft tissue injury related withsports activities including compartment syndrome, ankle sprain, knee ligament injury, and muscle contusion.

(6)

Publications[Original Articles]
1. Saya Watanabe, Junya Aizawa, Manabu Shimoda, Mitsuhiro Enomoto, Tomomasa Nakamura, Atushi
Okawa, Kazuyoshi Yagishita. Eﬀect of short-term fatigue, induced by high-intensity exercise, on the
proﬁle of the ground reaction force during single-leg anterior drop-jumps. J Phys Ther Sci. 2016; 28(12);3371-3375
2. Toshifumi Watanabe, Takeshi Muneta, Kazuyoshi Yagishita, Kenji Hara, Hideyuki Koga, Ichiro Sekiya.
Closed Suction Drainage Is Not Necessary for Total Knee Arthroplasty: A Prospective Study on Simultaneous Bilateral Surgeries of a Mean Follow-Up of 5.5 Years. J Arthroplasty. 2016.03; 31(3); 641-645
3. YASUSHI KOJIMA, MITSUHIRO ENOMOTO, TAKUYA OYAIZU, KAZUYOSHI YAGISHITA. Whatis the optimal management of decompression illness?: Treatment at Medical Hospital of Tokyo Medical
and Dental University The Japanese Journal of Hyperbaric and Undersea Medicine. 2016.03; 51(1); 14-18
4. Kairi Hayashi, Toshiyuki Takahashi, Mai Ikegawa, Masaki Horie, Takuya Oyaizu, Mitsuhiro Enomoto,Shunichi Shibata, Kazuyoshi Yagishita, Toshiaki Ueno. The facilitatory eﬀects of hyperbaric oxygen
treatment on membrane bone wound healing in a rat calvarial defect model. Undersea Hyperb Med.
2016.03; 43(2); 135-142
5. Yoshiike T, Nishida M, Yagishita K, Nariai T, Ishii K, Nishikawa T.. Altered Sleep Spindles in Delayed
Encephalopathy after Acute Carbon Monoxide Poisoning. J Clin Sleep Med.. 2016.06; 12(6); 913-915
6. Suzuki H, Ito Y, Shinohara M, Yamashita S, Ichinose S, Kishida A, Oyaizu T, Kayama T, Nakamichi
R, Koda N, Yagishita K, Lotz MK, Okawa A, Asahara H.. Gene targeting of the transcription factor
Mohawk in rats causes heterotopic ossiﬁcation of Achilles tendon via failed tenogenesis. Proceedings of
the National Academy of Sciences of the United States of America. 2016.07; 113(28); 7840-7845
7. Hiroki Katagiri, Kaori Nakamura, Toshifumi Watanabe, Hideyuki Koga, Kazuyoshi Yagishita, Ichiro
Sekiya, Takeshi Muneta. Increase of patellofemoral height has decreased maximum knee ﬂexion after
total knee arthroplasty of posterior cruciate-substituting prosthesis in a clinical series. J Orthop Sci.
2016.07; 21(4); 458-462
8. Junya Aizawa, Shunsuke Ohji, Hideyuki Koga, Tadashi Masuda, Kazuyoshi Yagishita. Correlations between sagittal plane kinematics and landing impact force during single-leg lateral jump-landings. J Phys
Ther Sci. 2016.08; 28(8); 2316-2321
9. Yoko Yamamoto, Yoshihiro Noguchi, Mitsuhiro Enomoto, Kazuyoshi Yagishita, Ken Kitamura. Otologicalcomplications associated with hyperbaric oxygen therapy. Eur Arch Otorhinolaryngol. 2016.09; 273(9);2487-2493
10. Toshitaka Yoshii, Kenichiro Sakai, Takashi Hirai, Tsuyoshi Yamada, Hiroyuki Inose, Tsuyoshi Kato,
Mitsuhiro Enomoto, Shoji Tomizawa, Shigenori Kawabata, Yoshiyasu Arai, Atsushi Okawa. Anterior
decompressionwithfusionversusposteriordecompressionwithfusionformassivecervicalossiﬁcationofthe
posteriorlongitudinalligamentwitha ≥50%canaloccupyingratio:amulticenterretrospectivestudy.SpineJ.2016.11;16(11);1351-1357
11. Kazuyoshi Yagishita, Tetsuya Jinno, Daisuke Koga, Tsuyoshi Kato, Mitsuhiro Enomoto, Tsuyoshi Kato,
Takeshi Muneta, Atsushi Okawa. Transient osteoporosis of the hip treated with hyperbaric oxygen therapy: a case series. Undersea Hyperb Med. 2016.11; 43(7); 847-854― 549 ―

University Hospitals

Central Clinical Facilities

12. Tomomasa Nakamura, Yuriko Yoshida, Hiroshi Churei, Junya Aizawa, Kenji Hirohata, Takehiro Ohmi,
Shunsuke Ohji, Toshiyuki Takahashi, Mitsuhiro Enomoto, Toshiaki Ueno, Kazuyoshi Yagishita. The Eﬀect
of Teeth Clenching on Dynamic Balance at Jump-Landing: A Pilot Study. J Appl Biomech. 2016.12;
1-19
13. Toshitaka Yoshii, Takashi Hirai, Kenichiro Sakai, Shinichi Sotome, Mitsuhiro Enomoto, Tsuyoshi Yamada,
Hiroyuki Inose, Tsuyoshi Kato, Shigenori Kawabata, Atsushi Okawa. Anterior Cervical Corpectomy and
Fusion Using a Synthetic Hydroxyapatite Graft for Ossiﬁcation of the Posterior Longitudinal Ligament.
Orthopedics. 2016.12; 1-6[Misc]
1. Mitsuhiro Enomoto, Takashi Hirai, Hidetoshi Kaburagi, Takanori Yokota. Eﬃcient Gene Suppressionin Dorsal Root Ganglia and Spinal Cord Using Adeno-Associated Virus Vectors Encoding Short-Hairpin
RNA. Methods Mol. Biol.. 2016; 1364; 277-290
2. Mitsuhiro Enomoto. Therapeutic eﬀects of neurotrophic factors in experimental spinal cord injury models
Journal of Neurorestoratology. 2016; (4); 15-22[Conference Activities & Talks]
1. Yasushi Kojima, Mitsuhiro Enomoto, Takuya Oyaizu, Akiko Kojima, Kazuyoshi Yagishita,N. Kagaya.
Recreational diving related fatalities in Japan (2004-2014). 45th South Paciﬁc Underwater Medicine
Society Annual Meeting 2016.05 Natadola coast, FIJI
2. Oyaizu T, Enomoto M, Yagishita K.. Hyperbaric and high-oxygen environments lead to macrophageinﬁltration, stimulate cell proliferation and accelerate muscle regeneration in contused rat skeletal muscle..
Undersea and Hyperbaric Medical Society Annual Scientiﬁc Meeting 2016 2016.06.09 Las Vegas, UnitedStates of America
3. Hidetoshi Kaburagi, Takashi Hirai, Takanori Yokota , Atsushi Okawa,Mitsuhiro Enomoto. Role ofvoltage-gated sodium channels and calcium channel subunit alpha-2/delta-1 in the lumbar dorsal root
ganglions of persistent peripheral neuropathic pain model mice.. Neuroscience2016 2016.11.15 San Diego― 550 ―

University
CentralClinical Hospitals
Facilities

Center for Cell Therapy

Director: Tomohiro Morio (Department of Pediatrics)
Vise Director: Ichiro Sekiya (Center for Stem Cell and Regenerative Medicine)
Quality control manager: Norio Shimizu (Center for Stem Cell and Regenerative Medicine)
Product manager: Michiko Kajiwara (Department of Blood Transfusion Medicine)Technicians: Yuri Kohno, Minhua Sun, Ayako Tsuji
Technicians (From Collaborative Research): Takafumi Kato, Takuya Okazaki
Clerical Assistant: Akiko Hoshikawa, Jun Kusano(1)

Research

1. Development of innovative techniques for quality assurance of cell products
2. Development of a novel measure for rapid and sensitive detection of multiple pathogens
3. Development of multi-virus speciﬁc T lymphocytes for adoptive immunotherapy
4. Research on a regeneration system of the cartilage bone from the synovial membrane (Department of Orthopedic Surgery)
5. Development of novel peptide-pulsed dendritic therapy for adult T-cell leukemia (Department of Immunotherapeutics / Department of Hematology)
We are planning to start multi-virus speciﬁc T cell therapy for opportunistic viral infection post hematopoietic
cell transplantation and expanded colon epithelium for various bowel disorders following approval from thecertiﬁed committee for regenerative medicine and from MHLW.

(2)

Education

We provide assistance to prepare standard operation procedure (SOP) and oﬀer on-the-job training for cellprocessing/ manipulating procedures and that for quality assurance at the center. Facility for the educationand training was recently installed at the CPC annex.
(3)

Clinical Services & Other Works

The cell products cuurently prepared in our center include#1 Synovium-derived mesenchymal stem cells#2 Proccessed peripheral blood stem cells
The center oﬀers our novel detection system for 12 diﬀerent viruses in rapid and sensitive manner for the
doctors at TMDU Medical Hospital. We also measure virus loads of the detected virus using a real time PCRsystem. We measured 1,190 samples until September, 2016. The multi-virus PCR system has been oﬀered by
Department of Clinical Laboratory thereafter.

― 551 ―

University
Hospitals
CentralClinicalFacilities(4)

Clinical Performances

Our center in TMDU Medical Hospital was renovated and re-started operation as of March 2015. We have ﬁve
independent cell processing rooms (class 10,000 clean rooms). All the rooms are equipped with a bio-safetycabinet. The hardware as well as software used in our center fulﬁlls all the guidelines that are required for the
preparation of cell products of clinical grade.
(5)

Publications[Original Articles]
1. Arai A, Sakashita C, Hirose C, Imadome K, Yamamoto M, Jinta M, Fujiwara S, Tomita M, Shimizu
N, Morio T, and Miura O.. Hematopoietic stem cell transplantation for adults with EBV-positive Tor NK-cell lymphoproliferative disorders: eﬃcacy and predictive markers Bone Marrow Transplantation.
2016;
2. T Wada, Toma T, M Yasui, M Inoue, K Kawa, K Imai, T Morio, A Yachie. Diﬀerent Clinical Phenotypes
in 2 Siblings With X-Linked Severe Combined Immunodeﬁciency. J Investig Allergol Clin Immunol. 2016;
26(1); 63-65
3. Tomohiro Kohmoto, Atsumi Tsuji, Kei-Ichi Morita, Takuya Naruto, Kiyoshi Masuda, Kenichi Kashimada,
Keisuke Enomoto, Tomohiro Morio, Hiroyuki Harada, Issei Imoto. A novel COL11A1 missense mutationin siblings with non-ocular Stickler syndrome. Hum Genome Var. 2016; 3; 16003
4. Kotaro Kai, Maki Sumida, Yaeko Motoyoshi, Yuichi Ogawa, Katsuyuki Miki, Kazuhiro Iwadoh, Akihito
Sannomiya, Toru Murakami, Ichiro Koyama, Kumiko Kitajima, Ichiro Nakajima, Tomohiro Morio, Shohei
Fuchinoue. Successful Long-term Graft Survival of a Renal Transplantation Patient with Wiskott-Aldrich
Syndrome. Intern. Med.. 2016; 55(13); 1761-1763
5. Yuki Aoki, Reiji Miyawaki, Kohsuke Imai, Masatoshi Takagi, Michiko Kajiwara, Yasuyoshi Ishiwata,
Masato Yasuhara, Tomohiro Morio, Shuki Mizutani, Daisuke Tomizawa. Haploidentical Bone Marrow
Transplantation With Clofarabine and Busulfan Conditioning for a Child With Multiple Recurrent Acute
Lymphoblastic Leukemia. J. Pediatr. Hematol. Oncol.. 2016.01; 38(1); 39-41
6. Seiichi Hayakawa, Satoshi Okada, Miyuki Tsumura, Sonoko Sakata, Yoshitaka Ueno, Kohsuke Imai,
Tomohiro Morio, Osamu Ohara, Kazuaki Chayama, Masao Kobayashi. A Patient with CTLA-4 Haploinsuﬃciency Presenting Gastric Cancer. J. Clin. Immunol.. 2016.01; 36(1); 28-32
7. Laura Gámez-Díaz, Dietrich August, Polina Stepensky, Shoshana Revel-Vilk, Markus G Seidel, Mitsuiki
Noriko, Tomohiro Morio, Austen J J Worth, Jacob Blessing, Frank Van de Veerdonk, Tobias Feuchtinger,
Maria Kanariou, Annette Schmitt-Graeﬀ, Sophie Jung, Suranjith Seneviratne, Siobhan Burns, Bernd H
Belohradsky, Nima Rezaei, Shahrzad Bakhtiar, Carsten Speckmann, Michael Jordan, Bodo Grimbacher.
The extended phenotype of LPS-responsive beige-like anchor protein (LRBA) deﬁciency. J. Allergy Clin.
Immunol.. 2016.01; 137(1); 223-230
8. T Muto, Y Takeda, S Tsukamoto, S Sakai, N Mimura, C Ohwada, M Takeuchi, E Sakaida, S Ota, T
Iseki, N Shimizu, T Morio, C Nakaseko. Successful treatment of cytomegalovirus enteritis after unrelated
allogeneic stem cell transplantation by the infusion of ex vivo-expanded CD4(+) lymphocytes derived
from the recipient’s peripheral blood donor cells. Transpl Infect Dis. 2016.02; 18(1); 93-97
9. Natsuko Inazawa, Tsukasa Hori, Masaki Yamamoto, Naoki Hatakeyama, Yuko Yoto, Masanori Nojima,
Hiroshi Yasui, Nobuhiro Suzuki, Norio Shimizu, Hiroyuki Tsutsumi. HHV-6 encephalitis may complicate
the early phase after allogeneic hematopoietic stem cell transplantation: Detection by qualitative multiplex
PCR and subsequent quantitative real-time PCR. J. Med. Virol.. 2016.02; 88(2); 319-323
10. Kei Takasawa, Sayaka Takeda, Masato Nishioka, Hiroshi Sakuma, Tomohiro Morio, Masayuki Shimohira.
Steroid-responsive Status Epilepticus Caused by Human Parvovirus B19 Encephalitis. Pediatr. Infect.
Dis. J.. 2016.02; 35(2); 227-228
11. Yusuke Nakagawa, Ichiro Sekiya, Shimpei Kondo, Takashi Tabuchi, Shizuko Ichinose, Hideyuki Koga,
Kunikazu Tsuji, Takeshi Muneta. Relationship between MRI T1 rho value and histological ﬁndings ofintact and radially incised menisci in microminipigs. J Magn Reson Imaging. 2016.02; 43(2); 434-445― 552 ―

University
CentralClinical Hospitals
Facilities

12. Yumiko Nakano, Takeshi Hayashi, Kentaro Deguchi, Kota Sato, Nozomi Hishikawa, Toru Yamashita,
Yasuyuki Ohta, Yoshiki Takao, Tomohiro Morio, Koji Abe. Two young stroke patients associated withregular intravenous immunoglobulin (IVIg) therapy. J. Neurol. Sci.. 2016.02; 361; 9-12
13. Kazuhiro Ikegame, Kohsuke Imai, Motoi Yamashita, Akihiro Hoshino, Hirokazu Kanegane, Tomohiro
Morio, Katsuji Kaida, Takayuki Inoue, Toshihiro Soma, Hiroya Tamaki, Masaya Okada, Hiroyasu Ogawa.
Allogeneic stem cell transplantation for X-linked agammaglobulinemia using reduced intensity conditioning
as a model of the reconstitution of humoral immunity. J Hematol Oncol. 2016.02; 9(1); 9
14. A Arai, C Sakashita, C Hirose, K-I Imadome, M Yamamoto, M Jinta, S Fujiwara, M Tomita, N Shimizu,
T Morio, O Miura. Hematopoietic stem cell transplantation for adults with EBV-positive T- or NK-cell
lymphoproliferative disorders: eﬃcacy and predictive markers. Bone Marrow Transplant.. 2016.02; 51(6);879-882
15. Yusuke Nakagawa, Takeshi Muneta, Koji Otabe, Nobutake Ozeki, Mitsuru Mizuno, Mio Udo, Ryusuke
Saito, Katsuaki Yanagisawa, Shizuko Ichinose, Hideyuki Koga, Kunikazu Tsuji, Ichiro Sekiya. Cartilage
Derived from Bone Marrow Mesenchymal Stem Cells Expresses Lubricin In Vitro and In Vivo. PLoS
ONE. 2016.02; 11(2); e0148777
16. Chisato Sato, Yoshitaka Iso, Takuya Mizukami, Koji Otabe, Masahiro Sasai, Masaaki Kurata, Takeyuki
Sanbe, Ichiro Sekiya, Akira Miyazaki, Hiroshi Suzuki. Fibroblast growth factor-23 induces cellular senescence in human mesenchymal stem cells from skeletal muscle. Biochem. Biophys. Res. Commun..
2016.02; 470(3); 657-662
17. Shimpei Kondo, Takeshi Muneta, Yusuke Nakagawa, Hideyuki Koga, Toshifumi Watanabe, Kunikazu
Tsuji, Shinichi Sotome, Atsushi Okawa, Shinji Kiuchi, Hideo Ono, Mitsuru Mizuno, Ichiro Sekiya. Transplantation of autologous synovial mesenchymal stem cells promotes meniscus regeneration in aged primates. J. Orthop. Res.. 2016.02;
18. Toshifumi Watanabe, Takeshi Muneta, Kazuyoshi Yagishita, Kenji Hara, Hideyuki Koga, Ichiro Sekiya.
Closed Suction Drainage Is Not Necessary for Total Knee Arthroplasty: A Prospective Study on Simultaneous Bilateral Surgeries of a Mean Follow-Up of 5.5 Years. J Arthroplasty. 2016.03; 31(3); 641-645
19. Masakatsu Yanagimachi, Takashi Ohya, Tomoko Yokosuka, Ryosuke Kajiwara, Fumiko Tanaka, Hiroaki
Goto, Takehiro Takashima, Tomohiro Morio, Shumpei Yokota. The Potential and Limits of Hematopoietic
Stem Cell Transplantation for the Treatment of Autosomal Dominant Hyper-IgE Syndrome. J. Clin.
Immunol.. 2016.04; 36(5); 511-516
20. Julie Toubiana, Satoshi Okada, Julia Hiller, Matias Oleastro, Macarena Lagos Gomez, Juan Carlos Aldave Becerra, Marie Ouachée-Chardin, Fanny Fouyssac, Katta Mohan Girisha, Amos Etzioni, Joris Van
Montfrans, Yildiz Camcioglu, Leigh Ann Kerns, Bernd Belohradsky, Stéphane Blanche, Aziz Bousﬁha,
Carlos Rodriguez-Gallego, Isabelle Meyts, Kai Kisand, Janine Reichenbach, Ellen D Renner, Sergio Rosenzweig, Bodo Grimbacher, Frank L van de Veerdonk, Claudia Traidl-Hoﬀmann, Capucine Picard, Laszlo
Marodi, Tomohiro Morio, Masao Kobayashi, Desa Lilic, Joshua D Milner, Steven Holland, Jean-Laurent
Casanova, Anne Puel. Heterozygous STAT1 gain-of-function mutations underlie an unexpectedly broadclinical phenotype: an international survey of 274 patients from 167 kindreds. Blood. 2016.04; 127(25);3154-3164
21. Katsuaki Yanagisawa, Takeshi Muneta, Nobutake Ozeki, Yusuke Nakagawa, Mio Udo, Ryusuke Saito,
Hideyuki Koga, Kunikazu Tsuji, Ichiro Sekiya. Weekly injections of Hylan G-F 20 delay cartilage degeneration in partial meniscectomized rat knees. BMC Musculoskelet Disord. 2016.04; 17(1); 188
22. Kazumasa Miyatake, Takeshi Muneta, Miyoko Ojima, Jun Yamada, Yu Matsukura, Kahaer Abula, Ichiro
Sekiya, Kunikazu Tsuji. Coordinate and synergistic eﬀects of extensive treadmill exercise and ovariectomy
on articular cartilage degeneration. BMC Musculoskelet Disord. 2016.05; 17(1); 238
23. Mai Katakura, Hideyuki Koga, Kaori Nakamura, Ichiro Sekiya, Takeshi Muneta. Eﬀects of diﬀerent
femoral tunnel positions on tension changes in anterolateral ligament reconstruction. Knee Surg Sports
Traumatol Arthrosc. 2016.05;
24. N Ozeki, T Muneta, H Koga, Y Nakagawa, M Mizuno, K Tsuji, Y Mabuchi, C Akazawa, E Kobayashi, K
Matsumoto, K Futamura, T Saito, I Sekiya. Not single but periodic injections of synovial mesenchymal
stem cells maintain viable cells in knees and inhibit osteoarthritis progression in rats. Osteoarthr. Cartil..
2016.06; 24(6); 1061-1070― 553 ―

University
Hospitals
CentralClinicalFacilities

25. Yuki Tsujita, Kanako Mitsui-Sekinaka, Kohsuke Imai, Tzu-Wen Yeh, Noriko Mitsuiki, Takaki Asano, Hidenori Ohnishi, Zenichiro Kato, Yujin Sekinaka, Kiyotaka Zaha, Tamaki Kato, Tsubasa Okano, Takehiro
Takashima, Kaoru Kobayashi, Mitsuaki Kimura, Tomoaki Kunitsu, Yoshihiro Maruo, Hirokazu Kanegane, Masatoshi Takagi, Kenichi Yoshida, Yusuke Okuno, Hideki Muramatsu, Yuichi Shiraishi, Kenichi
Chiba, Hiroko Tanaka, Satoru Miyano, Seiji Kojima, Seishi Ogawa, Osamu Ohara, Satoshi Okada, Masao
Kobayashi, Tomohiro Morio, Shigeaki Nonoyama. Phosphatase and tensin homolog (PTEN) mutation
can cause activated phosphatidylinositol 3-kinase δ syndrome-like immunodeﬁciency. J. Allergy Clin.
Immunol.. 2016.06; 138(6); 1672-1680
26. Hiroki Katagiri, Kaori Nakamura, Toshifumi Watanabe, Hideyuki Koga, Kazuyoshi Yagishita, Ichiro
Sekiya, Takeshi Muneta. Increase of patellofemoral height has decreased maximum knee ﬂexion after
total knee arthroplasty of posterior cruciate-substituting prosthesis in a clinical series. J Orthop Sci.
2016.07; 21(4); 458-462
27. Mio Udo, Takeshi Muneta, Kunikazu Tsuji, Nobutake Ozeki, Yusuke Nakagawa, Toshiyuki Ohara, Ryusuke
Saito, Katsuaki Yanagisawa, Hideyuki Koga, Ichiro Sekiya. Monoiodoacetic acid induces arthritis and synovitis in rats in a dose- and time-dependent manner: proposed model-speciﬁc scoring systems. Osteoarthr.
Cartil.. 2016.07; 24(7); 1284-1291
28. Natsuko Inazawa, Tsukasa Hori, Masanori Nojima, Makoto Saito, Keita Igarashi, Masaki Yamamoto,
Norio Shimizu, Yoko Yuto, Hiroyuki Tsutsumi. Virus reactivations after autologous hematopoietic stem
cell transplantation detected by multiplex PCR assay. J. Med. Virol.. 2016.07;
29. Shigeru Takishima, Keisuke Nakajima, Risa Nomura, Atsumi Tsuji-Hosokawa, Nozomi Matsuda, Yohei
Matsubara, Makoto Ono, Kentaro Miyai, Kei Takasawa, Tomohiro Morio, Yukihiro Hasegawa, Kenichi
Kashimada. Lower body weight and BMI at birth were associated with early adiposity rebound in 21hydroxylase deﬁciency patients. Endocr. J.. 2016.08; 63(11); 983-990
30. Katsutsugu Umeda, Souichi Adachi, Shiro Tanaka, Mizuka Miki, Keiko Okada, Yoshiko Hashii, Masami
Inoue, Yuko Cho, Katsuyoshi Koh, Hiroaki Goto, Ryosuke Kajiwara, Nobuyuki Hyakuna, Koji Kato,
Tomohiro Morio, Hiromasa Yabe, . Comparison of second transplantation and donor lymphocyte infusion
for donor mixed chimerism after allogeneic stem cell transplantation for nonmalignant diseases. Pediatr
Blood Cancer. 2016.08; 63(12); 2221-2229
31. Shumpei Yokota, Yasuhiko Itoh, Tomohiro Morio, Hideki Origasa, Naokata Sumitomo, Minako Tomobe,
Kunihiko Tanaka, Seiji Minota. Tocilizumab in systemic juvenile idiopathic arthritis in a real-world
clinical setting: results from 1 year of postmarketing surveillance follow-up of 417 patients in Japan. Ann.
Rheum. Dis.. 2016.09; 75(9); 1650-1660
32. Takeshi Muneta, Hideyuki Koga, Tomomasa Nakamura, Masafumi Horie, Toshifumi Watanabe, Ichiro
Sekiya. Behind-remnant arthroscopic observation and scoring of femoral attachment of injured anterior
cruciate ligament. Knee Surg Sports Traumatol Arthrosc. 2016.09; 24(9); 2906-2914
33. Takeshi Sato, Tsubasa Okano, Mari Tanaka-Kubota, Shunsuke Kimura, Satoshi Miyamoto, Shintaro Ono,
Motoi Yamashita, Noriko Mitsuiki, Masatoshi Takagi, Kohsuke Imai, Michiko Kajiwara, Takasuke Ebato,
Shohei Ogata, Hirotsugu Oda, Osamu Ohara, Hirokazu Kanegane, Tomohiro Morio. Novel compound
heterozygous mutations in a Japanese girl with Janus kinase 3 deﬁciency. Pediatr Int. 2016.09; 58(10);1076-1080
34. Koga H, Muneta T, Watanabe T, Mochizuki T, Horie M, Nakamura T, Otabe K, Nakagawa Y, Sekiya I..
Two-Year Outcomes After Arthroscopic Lateral Meniscus Centralization Arthroscopy. 2016.10; 32(10);2000-2008
35. Shotaro Ando, Jun-Ichi Kawada, Takahiro Watanabe, Michio Suzuki, Yoshitaka Sato, Yuka Torii, Masato
Asai, Fumi Goshima, Takayuki Murata, Norio Shimizu, Yoshinori Ito, Hiroshi Kimura. Tofacitinib induces
G1 cell-cycle arrest and inhibits tumor growth in Epstein-Barr virus-associated T and natural killer cell
lymphoma cells. Oncotarget. 2016.11; 7(47); 76793-76805
36. Watanabe T, Muneta T, Koga H, Horie M, Nakamura T, Otabe K, Nakagawa Y, Katakura M, Sekiya I..
In-vivo kinematics of high-ﬂex posterior-stabilized total knee prosthesis designed for Asian populations.
Int Orthop. 2016.11; 40(11); 2295-2302― 554 ―

University
CentralClinical Hospitals
Facilities

37. Akifumi Endo, Daisuke Tomizawa, Yuki Aoki, Tomohiro Morio, Shuki Mizutani, Masatoshi Takagi.
EWSR1/ELF5 induces acute myeloid leukemia by inhibiting p53/p21 pathway. Cancer Sci.. 2016.12;
107(12); 1745-1754
38. Kaori Nakamura, Hideyuki Koga, Ichiro Sekiya, Toshifumi Watanabe, Tomoyuki Mochizuki, Masafumi
Horie, Tomomasa Nakamura, Koji Otabe, Takeshi Muneta. Dynamic Evaluation of Pivot-Shift Phenomenon in Double-Bundle Anterior Cruciate Ligament Reconstruction Using Triaxial Accelerometer.
Arthroscopy. 2016.12; 32(12); 2532-2538
39. Inoue M, Muneta T, Ojima M, Nakamura K, Koga H, Sekiya I, Okazaki M, Tsuji K.. Inﬂammatory
cytokine levels in synovial ﬂuid 3, 4 days postoperatively and its correlation with early-phase functional
recovery after anterior cruciate ligament reconstruction: a cohort study. J Exp Orthop.. 2016.12; 3(1);30
40. Toshiyuki Ohara, Takeshi Muneta, Yusuke Nakagawa, Yu Matsukura, Shizuko Ichinose, Hideyuki Koga,
Kunikazu Tsuji, Ichiro Sekiya. < Original Article> Hypoxia enhances proliferation through increase ofcolony formation rate with chondrogenic potential in primary synovial mesenchymal stem cells. J. Med.
Dent. Sci.. 2016.12; 63(4); 61-70
41. Daisuke Tomizawa, Akifumi Endo, Michiko Kajiwara, Hirotoshi Sakaguchi, Kimikazu Matsumoto, Makoto
Kaneda, Takashi Taga. Acute lymphoblastic leukemia in patients with Down syndrome with a previous
history of acute myeloid leukemia. Pediatr Blood Cancer. 2016.12;[Conference Activities & Talks]
1. Koji Otabe, Ken Watanabe, Norio Shimizu, Takeshi Muneta, Ichiro Sekiya. High Sensitivity Virus /Mycoplasma Screening Test Reveals High Prevalence of Parvovirus B19 Infection In Synovial Tissues andBone Marrow. Orthopaedic Research Society 2016 Annual Meeting 2016.03.06 Orlando, USA
2. Mitsuru Mizuno, Hisako Katano, Koji Otabe, Keiichiro Komori, Yukie Matsumoto,Nobutake Ozeki, Kunikazu Tsuji, Takeshi Muneta, Ichiro Sekiya. PDGF-AA/AB In Human Serum Are Potential Indicators Of
The Proliferative Capacity Of Human Synovial Mesenchymal Stem Cells. Orthopaedic Research Society
2016 Annual Meeting 2016.03.06 Orlando, USA
3. Mitsuru Mizuno, Hisako Katano, Yo Mabuchi, Yusuke Ogata, Shizuko Ichinose, Koji Otabe, Keiichiro
Komori, Yukie Matsumoto, Nobutake Ozeki, Kunikazu Tsuji, Chihiro Akazawa, Takeshi Muneta, Ichiro
Sekiya. Properties of MSCs Derived From Surface, Stroma, and Perivascular Synovium Of Oa Patients.
Orthopaedic Research Society 2016 Annual Meeting 2016.03.06 Orlando, USA
4. Kenta Katagiri, Takeshi Muneta, Koji Otabe, Yu Matsukura, Hisako Katano,Ichiro Sekiya. Fibrous
Synovium Releases Higher Number Of MSCs Than Adipose Synovium in a Suspended Synovium Culture
Model. Orthopaedic Research Society 2016 Annual Meeting 2016.03.06 Orlando, USA
5. Mana Naritomi, Mitsuru Mizuno, Hisako Katano, Koji Otabe, Nobutake Ozeki, Kunikazu Tsuji, Takeshi
Muneta, Ichiro Sekiya. Interleukin-1 β Promotes In Vitro Chondrogenesis Of Synovial Mesenchymal StemCells. Orthopaedic Research Society 2016 Annual Meeting 2016.03.06 Orlando, USA
6. Nobutake Ozeki, Junpei Matsuda, Takeshi Muneta, Hideyuki Koga, Kunikazu Tsuji, Kenta Katagiri,
Mitsuru Mizuno, Tomoyuki Saito, Ichiro Sekiya. Biomechanical Analysis of Centralization With an Anchor
for Meniscus Extrusion in a Porcine Model. Orthopaedic Research Society 2016 Annual Meeting 2016.03.07
Orlando, USA
7. Yusuke Nakagawa, Hideyuki Koga, Takeshi Muneta, Shinji Kiuchi, Hideo Ono, Tsuyoshi Nagata, Toshifumi Watanabe, Masafumi Horie, Masafumi Horie, Tomomasa Nakamura, Koji Otabe, Mai Katakura,
Hisako Katano, Ichiro Sekiya. Three-Dimensional Reconstructed MRI Evaluation of Arthroscopic Centralization Technique for Extruded Lateral Meniscus. Orthopaedic Research Society 2016 Annual Meeting2016.03.07 Orlando, USA
8. Ichiro Sekiya. Adult stem cell-based approaches (Intraarticular delivery, model systems vs humans)
Overview. ICRS Summit 2016.04.10 Kyoto, JAPAN― 555 ―

University
Hospitals
CentralClinicalFacilities

9. Haruna Shibayama, Ken-ichi Imadome, Chizuko Sakashita, Ken Watanabe, Norio Shimizu, Takatoshi
Koyama, Shigeyoshi Fujiwara, Osamu Miura, Ayako Arai. In vitro and in vivo eﬀects of proteasome
inhibitor bortezomib on Epstein-Barr virus-positive T- or NK-cell lymphoproliferative diseases. 17th
International Symposium on Epstein-Barr virus (EBV) and Associated Diseases 2016.08.10 Zurich
10. Shiho KOBAYASHI, Naoki OTOMO, Kaoru OKUYAMA, Shihoko SUWA, Michiko KAJIWARA. Studyof the performance and usefulness of "ORTHO VISION Max" in TMDU. The 32nd World Congress ofBiomedical Laboratory Science 2016.09.02 Kobe International Conference Center, JAPAN11. Mitsui-Sekinaka K, Imai K, Tsujita Y, Mitsuiki N, Asano T, Sekinaka Y, Kanegane H, Yoshida K, Miyano
S, Kojima S, Ogawa S, Ohara O, Okada S, Kobayashi M, Takagi M, Morio T, Nonoyama S.. Activated
PI3 Kinase Delta Syndrome (APDS)-Like Immunodeﬁciency Caused by PTEN Mutation. 17th Biennial
Meeting of the European Society for Immunodeﬁciencies 2016.09.22 Barcelona, Spain
12. Hoshino A, Okada S, Yoshida K, Nishida N, Yamashita M, Okano T, Tsumura M, Nishimura S, Sakata
S, Takagi M, Imai K, Miyano S, Ogawa S, Kojima S, Nonoyama S, Morio T, Kanegane H. . Abnormal
Hematopoiesis and Autoimmunity in Humans with Germline IKZF1 Mutations. 17th Biennial Meeting of
the European Society for Immunodeﬁciencies 2016.09.22 Barcelona, Spain
13. Leiding J,Okada S,Shcherbina A,Abinun M,Pulsipher M,Hagin D,Linemans C,Sullivan K,Bunin N,Kilic
S,De La Calle-Martin O,Roiman C,Aldave J.C,Iguchi A,Imai K,Ohkawa T,Gennery A,Slatter M,Ochs
H,Mori T,Torgerson T. Hematopoietic Stem cell Transplantation in Patients with Gain of Function STAT1
Mutation. 17th Biennial Meeting of the European Society for Immunodeﬁciencies 2016.09.22 Barcelona,
Spain
14. Sekinaka Y,Mitsuiki N,Imai K,Yabe M,Mitsui-Sekinaka K,Honma K,Arai A,Yoshida K,Miyano S,Seiji
K,Hira A,Takata M,Ohara O,Ogawa S,Morio T,Nonoyama S. Common Variable Immunodeﬁciency Caused
by Fanc Mutations. 17th Biennial Meeting of the European Society for Immunodeﬁciencies 2016.09.22
Brcelona, Spain
15. Ichiro Sekiya. Scientiﬁc update: the synovial joint milieu - implications for cartilage repair. ICRS World
Congress 2016.09.23 Naples,Italy
16. Okano T, Watanabe E, Takashima T, Nishikawa T, Kawano Y, Tomoda T, Tanaka-Kubota M, Miyamoto
S, Yeh T, Yamashita M, Tanaka K, Mitusiki N, Ohara O, Takagi M, Imai K, Kanegane H, Morio T..
IGG1 Gammopathy in X-SCID Caused by Maternal T and B cell Engraftment. 17th Biennial Meeting of
the European Society for Immunodeﬁciencies 2016.09.23 Barcelona, Spain
17. Moriya K, Tanita K, Ohnishi H, Niizuma H, Rikiishi T, Nishikomori R, Ishige T, Imai K, Kanegane
H, Sasahara Y, Arakawa H, Kure S, Morio T. I < kappa> B-α S32 Mutations Underly Ectodermal
Dysplasia with Immunodeﬁciency Manifestations and Correlation with Immunological Parameters.. 17th
Biennial Meeting of the European Society for Immunodeﬁciencies 2016.09.23 Brcelona, Spain
18. Haruna Shibayama, Ken-ichi Imadome, Chizuko Sakashita, Ken Watanabe, Fuyuko Kawano, Norio Shimizu,
Takatoshi Koyama, Shigeyoshi Fujiwara, Osamu Miura, Ayako Arai. In vitro and in vivo eﬀects of bortezomib on EBV-T/NK-LPDs. The 78th Annual Meeting of the Japanese Society of Hematology 2016.10.14
Yokohama
19. Akihiro Hoshino, Satoshi Okada, Kenichi Yoshida, Naonori Nishida, Yusuke Okuno, Hiroo Ueno, Motoi Yamashita, Tsubasa Okano, Miyuki Tsumura, Shiho Nishimura, Sonoko Sakata, Masatoshi Takagi,
Kohsuke Imai, Yuichi Shiraishi, Kenichi Chiba, Hiroko Tanaka, Satoru Miyano, Seishi Ogawa, Seiji Kojima, Shigeaki Nonoyama, Tomohiro Morio, Hirokazu Kanegane. ABNORMAL HEMATOPOIESIS AND
AUTOIMMUNITY IN HUMANS WITH GERMLINE IKZF1 MUTATIONS. 2016.10.21
20. Marie Scully, Paul Knoebl, MD, Karim Kentouche, Lawrence Rice, Jerzy Windyga, Reinhard Schneppenheim, Johanna A Kremer Hovinga, Michiko Kajiwara, Caterina Maggiore, Jennifer Doralt, Leah Martell,and Bruce M. Ewenstein. Pharmacodynamic Proﬁle of a Recombinant ADAMTS13 (BAX930) in Hereditary Thrombotic Thrombocytopenic Purpura (Upshaw-Schulman Syndrome (USS)). American Society of
Hematology, 58th Annual Meeting 2016.12.03 San Diego, CA, USA
21. Akihiro Hoshino, Satoshi Okada, Kenichi Yoshida, Yusuke Okuno, Miyuki Tsumura, Shiho Nishimura,
Sonoko Sakata, Masatoshi Takagi, Kohsuke Imai, Yuichi Shiraishi, Kenichi Chiba, Hiroko Tanaka, Satoru― 556 ―

University
CentralClinical Hospitals
Facilities

Miyano, Seishi Ogawa, Seiji Kojima, Shigeaki Nonoyama, Tomohiro Morio, Hirokazu Kanegane. ABNORMAL HEMATOPOIESIS AND AUTOIMMUNITY IN HUMANS WITH GERMLINE IKZF1 MUTATIONS. 2016.12.16[Awards & Honors]
1. Ichiro Sekiya, The Japanese Society for Regenerative Medicine Award (Clinical Category), The JapaneseSociety for Regenerative Medicine, 2016.03
2. Koji Otabe, Excellence in Translational Science Award from Journal of Orthopaedic Research, Orthopaedic
Research Society, 2016.03
3. Mitsuru Mizuno, Young Investigator Award, The Japanese Tissue Culture Association, 2016.05
4. Yusuke Nakagawa, Grant for Study Abroad by Mitsukoshi Health and Welfare Foundation, Mitsukoshi
Health and Welfare Foundation, 2016.08― 557 ―

University Hospitals

Clinics for General Dentistry

Cleanroom

Associate Professor SUNAKAWA Mitsuhiro
Assistant Professor MATSUMOTO Hiroyuki(1)Research

1) The development of disposalble hygienic materials for dental use.
2) The survey for the oral diseases in patients with HIV.
3) The survey for the relationship between the consousness of the staﬀ and students and the needle stick accident
in the hospital.

(2)

Education

The improvement of the nosocominal infection control system in the University Hospital, Faculty of Dentistry,
Tokyo Medical and Dental University and the education of the actual infection control method to all staﬀ andclinical course students.
(3)

Publications[Misc]
1. Sunakawa M. Infection Control in Deentistry Carlisle. 2016.12; 21(3); 1-3[Conference Activities & Talks]
1. Iino Y, Yoshioka T, Hanada T, Ebihara A, Sunakawa M, Sumi Y, Okiji T. Visualization of substructuresof resected apical root surface by swept-source optical coherence tomography. The 15th Congress of theWorld Federation for Laser Dentistry 2016.07.17 Nagoya
2. Rashed BA, Ebihara A, Iino Y, Yoshioka T, Hanada T, Sunakawa M, Sumi Y, Okiji T. Evaluation of root
canal anatomy of maxillary premolars using swept source optical coherence tomography in comaparison
with dental operating microscope and cone beam computed tomography. The 15th Congress of the World
Federation for Laser Dentistry 2016.07.18 Nagoya
3. Kagaya N, Sawa E, Kobayashi N, Hanaoka S, Kobayashi M, Matsumoto H, Suzuki H. Patient’s attitude
survey to "harmful rumor" of HIV/AIDS. The 33rd Annual Meeting of The Japan Society for Disability
and Oral Hearlth 2016.10.02 Omiya
4. Iino Y, Ebihara A, Yoshioka T, Hanada T, Sunakawa M, Sum Y, Okiji T. Application of optical coherence
tomography in endodontics. The 23rd General Meeting of the Japanese Association for Dental Science2016.10.21 Fukuoka
5. Sunakawa M. Sterilization and disinfection methods of the used instruments in the dental clinic -actuals
at the dental hospital, Tokyo Medical and Dental University-. Instrument Management & Infection
Prevention Synposium 2016.10.30 Tokyo― 558 ―

University
CentralClinical Hospitals
Facilities

Center for Development of Devices and Drugs in Dentistry

Director Junji TAGAMI
Co-Director Hidekazu TAKAHASHI
Hideki HARASAWA
Naoko HARADA
Member Miwako WAGAI (CRC)
Kazuko KOJIMA (CRC)(1)

OutlineCenter for development of devices and drugs in dentistry was established in April, 2004 and is committed to awide range of activities, such as education, consultation for new devices and drugs application, and support forclinical trials in University Hospital of Dentistry.
(2)

Education

We provide a program for the 3rd year students of the School of Dentistry, also for the 2nd year students of
the School of Oral Health Care Sciences majoring in Oral Health Engineering to help them to gain fundamental
knowledge of Pharmaceutical Aﬀairs Act which is required for development and application of dental devices.
Collaborating with the Institute of Biomaterials and Bioengineering, we lecture the 1st year students in Masters Program at Graduate School of Medical and Dental Sciences on issues and systems related to the missionthat many outcomes from studies about innovative dental devices and materials will be put into use without
device-lag .

(3)

Clinical Services & Other Works

1. Clinical trial supporting Services
In order to accomplish clinical trials successfully, we manage and support from planning, paper work to patientcare as a main oﬃce of clinical trials in University Hospital of Dentistry.
2. Consultation Services
We provide consultation services about various issues concerning the Pharmaceutical Aﬀairs Act, not only forpharmaceutical and dental companies but also for dentists and researchers in our University.
By the supporting services of clinical trials, we hope that applicant will be able to form a protocol adequately
and eﬀectively, and to start the clinical trial swiftly.

(4)

Clinical Performances· Consultation achievements
We managed and supported one clinical trial for dental device in 2016.

― 559 ―

University
Hospitals
CentralClinicalFacilities

The 37 consultation services concerning dental devices were performed in 2016.

(5)

Publications[Original Articles]
1. Miho Sugiura, Yuichi Kitasako, Alireza Sadr, Yasushi Shimada, Yasunori Sumi, Junji Tagami. White
spot lesion remineralization by sugar-free chewing gum containing bio-available calcium and ﬂuoride: A
double-blind randomized controlled trial. Journal of Dentistry. 2016.11; 54; 86-91[Books etc]
1. Junji Tagami. Quarterly dental care. First publication, 2016.04
2. ryo imai,ritu aoki,reiko kaduki,hiko hyakusoku. rehabilitation makeup-To be visible makeup therapy
option-. kokuseidou publishing company, 2016.06
3. nico. Quintessence publishing, 2016.08[Misc]
1. Hidekazu TAKAHASHI. Outlook of CAD/CAM resin blocks approved by social insurance system Journalof Japan Academy of Digital Dentistry. 2016.07; 6(1); 3-6[Conference Activities & Talks]
1. Hidekazu TAKAHASHI. General inofmation for CAD/CAM composite resin blocks.. Project seminar ofJapanese Academy of Digital Dentistry 2016.02.21 Tokyo
2. Yuan Zhou, Khairul Matin. Evaluation of Resin Inﬁltration to Prevent Progression of Root Caries.
American Association for Dental Research (AADR) Annual Meeting 2016.03.16 Los Angeles, Calif, USA
3. Chihiro MATSUURA, Yasushi SHIMADA, Alireza SADR, Junji TAGAMI, Yasunori SUMI . 3D assessment of dentin caries under composite restorations using SS-OCT . 45th annual meeting of AADR
2016.03.17 Los Angeles
4. Tomoko TABATA, Yasushi SHIMADA, Alireza SADR, Junji TAGAMI, Yasunori SUMI. Assessment ofEnamel Crack at Cavosurface Margin Using 3D SS-OCT. 45th annual meeting of AADR 2016.03.17 Los
Angeles
5. Yuki Naruse, Tomohiro Tkakagaki, Naoko Matsui, Takaaki Sato, Toru Nikaido, Masaomi Ikeda, Junji
Tagami. Eﬀects of alumina-blasting for a CAD/CAM resin block on adhesion. 45th Annual meeting ofAmerican Association for Dental Research 2016.03.17 Los Angels
6. Masatoshi Nakajima, Ornnicha Thanatvarakorn, Taweesak Prasansuttiporn, Keiichi Hosaka, Junji Tagami.
Eﬀect of smear layer-deproteinizing on dentin bonding of self-etch adhesives. 45th AADR 2016.03.18 Los
Angeles, Calif
7. Taweesak Prasansuttiporn, Ornnicha Thanatvarakorn, Junji Tagami, Masatoshi Nakajima. Bonding durability of dental adhesive to smear layer-deproteinized dentin. 45th AADR 2016.03.18 Los Angeles, Calif
8. Ornnicha Thanatvarakorn, Taweesak Prasansuttiporn, Masahiro Takahashi, Suppason Thittaweerat, Junji
Tagami, Masatoshi Nakajima. Scrubbing eﬀect of one-step self-etch adhesives on dentin bonding. 45th
AADR 2016.03.18 Los Angeles, Calif
9. David Pashley, Ayaka Chiba, Jianfeng Zou, Masatoshi Nakajima, Jianguo Tan, Junji Tagami, Debora
Scheﬀel, Josimeri Heblingm, Kelli Agee, Lorenzo Breschi, Genevieve Gregorie, Seung Jang, Franklin Tay.
Eﬀect of ethanol on size-exclusion characteristics of dentin-collagen to monomer. 45th AADR 2016.03.18
Los Angeles, Calif.
10. Inoue G, Mashiko R, Nakashima S, Tagami J. Surface assessment on artiﬁcial caries-aﬀected dentin with
FCP-COMPLEX. 45th Annual Meeting of American Association for Dental Research 2016.03.19 Los
Angeles, USA― 560 ―

University
CentralClinical Hospitals
Facilities

11. Prof. Nairn H F Wilson. History and reasons behind current trends in dentistry in the UK. 2016.04.04
Tokyo medical and dental university
12. Junji Tagami. About composite resin restration. 2016.05.18
13. Microtensile bond strength to dentin of a newly developed one-step self-etch system containing hydrophilic
amide monomer. 2016.06.09
14. Sae AKEHASHI, Rena TAKAHASHI, Toru NIKAIDO, Junji TAGAMI. Enhancement of Dentin Bond
Strength of Resin Cement using New Resin Coating Materials. The 144th Meeting of the Japanese Societyof Conservative Dentistry 2016.06.10 Tochigi, Japan
15. Aramaki O, Kawashima N, Shimada Y, Okiji T, Tagami J . Three-dimensional analysis of Iba1+ macrophages
in human dental pulp using whole mount immunostaining. 94th General session & exhibition of the IADR
2016.06.22 Seoul, Korea
16. Rena Takahashi, Yasushi Shimada, Yu-Chih Chiang, Ikumi Wada, Yasunori Sumi, Junji Tagami. Comparison of light-cured pit and ﬁssure sealants on enamel demineralization using swept-source optical coherence
tomography. 94th General Session & Exhibition of the IADR 2016.06.24 Seoul, Korea
17. Yu-Jung Lai, Ting-Chen Liu, Szu-Ying Huang, Rena Takahashi, Yasushi Shimada, Junji Tagami, Yu-Chih
Chiang. Eﬀects of S-PRG Fillers-containing Composite on Demineralization Inhibition of Enamel Surface:
Optical Coherence Tomography (OCT) and Micro Computed Tomography (Micro CT) Approaches. 94th
General Session & Exhibition of the IADR 2016.06.24 Seoul, Korea
18. Eﬀect of cavity depth on Ultimate Tensile Strength of bulk ﬁll ﬂowable resin. 2016.09.10
19. Nakajima Y, Iwasaki N, Takahashi H, Yoshida M, Honda E, Kurabayashi T. MRI Artifacts and Radiopacity of CAD/CAM Composite Resin Blocks. The 2016 Academy of Dental Materials Annual Meeting2016.10.13 Chicago
20. Takahashi H, Iwasaki N, Sumi Y. Eﬀect of Cavity Design on Gap Formation Observed by OCT. The 2016
Academy of Dental Materials Annual Meeting 2016.10.14 Chicago
21. Takahashi H, Iwasaki N, Yasue T, Sumi Y. Observation of dental restoration gap formation using optical coherence tomography. 1st International Symposium on Creation of Life Innovation Materials forInterdisciplinary and International Researcher 2016.10.17 Suita, Osaka, Japan
22. Junji Tagami. Creating a future dentistry with the adhesive dentistry. Research Day with CU,UI,UMPandTMDU
Dental Students 2016 2016.10.18 Akio Suzuki memorial Hall in TMDU
23. Yuna KANAMORI, Rena TAKAHASHI, Toru NIKAIDO, Junji TAGAMI. The eﬀect of light irradiation on dentin bond strengths of dual-cure resin cements. The 145th Meeting of Japanese Society ofConservative Dentistry 2016.10.27 Nagano, Japan
24. Junji Tagami. New trend of dental materials, adehesive resin and composite resin. 2016.11.23 Study
medicine in Czech Republic
25. The evaluation of bond strength of calcium containing adhesive system to artiﬁcial demineralized dentin.
[Awards & Honors]
1. Best Poster Award (3rd prize), International Academy for Digital Dental Medicine, 2016.09― 561 ―

Sports Science Organization

Sports Science Organization

Clinical Center for Sports Medicine and Sports Dentistry

Clinical Center of Sports Medicine
Center Chief and Junior Associate Professor ; Kazuyoshi YAGISHITA
Junior Associate Professor ; Mitsuhiro ENOMOTOTokunin Assistant; Tomomasa NAKAMURA
Chief of Athletic Rehabilitation; Junya AIZAWA
Physiotherapist; Kenji HIROHATA, Takehiro OHMI, Shunsuke OHJI
Secretary; Kiyomi ITOHSports Medicine/Dentistry
Associate Professor; Toshiaki UENO
Assistant Professor;Toshiyuki TAKAHASHI, Hiroshi CHUREI
Hospital Staﬀ; atsuhide KUROKAWA
Graduate Student; Akihiro MITSUYAMA, Sintaro FUKASAWA,
Abhishekhi SHRESTHA,Mai IKEGAWA, Takahiro SHIRAKO,
Yuriko YOSHIDA,Nana SHIOTA,Hajime TANABE(1)

OutlineCenter of Sports Medicine and Sports Dentistry was established as a bridgehead for sports medical science andsports dental science which deals the clinical management of trauma and disorder for athletes and sports-active
people, and the safety measures and prevention of sports-related traumatic injuries and disorders. Center ofSports Medicine and Sports Dentistry is consisted of Clinical Center of Sports Medicine in University Hospitalof Medicine and Sports Medicine/Dentistry and Sports dentistry clinic in University Hospital of Dentistry.
(2)

Research○ Clinical Center of Sports Medicine
1) Athletic rehabilitation for rapid recovery from injury and high performance in athletes.
1)-a Intervention of core strength in patients with anterior cruciate ligament reconstruction.
1)-b Treatment from the aspect of core function in patients with overuse and fatigue fracture.
2) Evaluation methods for core function.
3) Development of dynamic stability.
4) Hyperbaric oxygen treatment4)-a Soft tissue injuries related with sports activities.
4)-b Conditioning in sports activities○ Sports Medicine/Dentistry1) Oral health promotion of athletes and sports-active people1)-a Field survey of oral health conditions in athletes and sports-active people1)-b Changes of oral environment associated with physical and sporting activities1)-c Inﬂuences of sports drinks and supplements on oral health2) Safety measures of sports-related dental and maxillofacial traumatic injuries2)-a Diagnosis and treatment techniques for sports-related dental and maxillofacial injuries2)-b Development and innovation of sports mouthguard― 562 ―

Sports Science Organization

Sports Science Organization2)-c Development and innovation of sports faceguard2)-d Development and innovation of scuba diving mouthpiece3) Correlations between occlusion and general motor functions3)-a Biomechanical assessment of motor performance associated with occlusion3)-b Electrophysiological analysis of neuromuscular function associated with occlusion
4) Correlations between occlusion and body posture5) Relations between mastication and occlusion and brain functions
6) Application of HBO therapy to sports-related dental diseases and traumatic injury(3)

Clinical Services & Other Works

Center of Sports Medicine and Sports Dentistry clinic oﬀers comprehensive care and clinical management forathletes and sports-active people suﬀered traumatic injuries, overuse disorders, disorders related with internal
medicine, and dental diseases.
○ Clinical Center of Sports Medicine
Number of patients (From January 2015 to December 2015)
Section of out-patient clinic: 4,480
Section of athletic rehabilitation: 3,747○ Sports Medicine/Dentistry, Sports dentistry clinic
Sports dentistry clinic oﬀers comprehensive care and clinical management for athletes and sports-active people
suﬀered dental diseases and traumatic injuries. Custom-ﬁtted protective gears such as mouthguard and faceguard against sports-related dental and maxillofacial trauma are also handled for participants in contact sports
such as a boxing, American football, rugby football, hockey, lacrosse, and martial art.

(4)

Publications[Original Articles]
1. Takashi Ikeda, Tetsuya jinnno, Junya Aizawa, Tadashi Masuda, Kazuo Hirakawa, Kazunari Ninomiya,Kouji Suzuki, Sadao Morita. Eﬀects of perioperative factors and hip geometry on hip abductor muscle
strength during the ﬁrst 6 months after anterolateral total hip arthroplasty. Journal of Physical Therapy
Science. 2016; in press;
2. T Nakamura, Y Yoshida, H Churei, J Aizawa, K Hirohata, T Ohmi, S Ohji, T Takahashi, M Enomoto,
T Ueno, K Yagishita. The eﬀect of teeth clenching on dynamic balance at jumplanding: a pilot study
Journal of Applied Biomechanics. 2016;
3. Saya Watanabe, Junya Aizawa, Manabu Shimoda, Mitsuhiro Enomoto, Tomomasa Nakamura, Atushi
Okawa, Kazuyoshi Yagishita. Eﬀect of short-term fatigue, induced by high-intensity exercise, on the
proﬁle of the ground reaction force during single-leg anterior drop-jumps. J Phys Ther Sci. 2016; 28(12);3371-3375
4. Toshifumi Watanabe, Takeshi Muneta, Kazuyoshi Yagishita, Kenji Hara, Hideyuki Koga, Ichiro Sekiya.
Closed Suction Drainage Is Not Necessary for Total Knee Arthroplasty: A Prospective Study on Simultaneous Bilateral Surgeries of a Mean Follow-Up of 5.5 Years. J Arthroplasty. 2016.03; 31(3); 641-645
5. YASUSHI KOJIMA, MITSUHIRO ENOMOTO, TAKUYA OYAIZU, KAZUYOSHI YAGISHITA. Whatis the optimal management of decompression illness?: Treatment at Medical Hospital of Tokyo Medical
and Dental University The Japanese Journal of Hyperbaric and Undersea Medicine. 2016.03; 51(1); 14-18
6. Hayashi K, Takahashi T, Tanabe M, Horie M, Oyaizu T, Enomoto M, Shibata S, Yagishita K, Ueno T.
The facilitatory eﬀects of hyperbaric oxygen treatment on membrane bone wound healing in a rat calvarial
defect model Undersea Hyperb Med. 2016.03; 43(2); 135-142
7. Kairi Hayashi, Toshiyuki Takahashi, Mai Ikegawa, Masaki Horie, Takuya Oyaizu, Mitsuhiro Enomoto,Shunichi Shibata, Kazuyoshi Yagishita, Toshiaki Ueno. The facilitatory eﬀects of hyperbaric oxygen― 563 ―

Sports Science Organization

Sports Science Organization

treatment on membrane bone wound healing in a rat calvarial defect model. Undersea Hyperb Med.
2016.03; 43(2); 135-142
8. Takashi Ikeda, Junya Aizawa, Hiroshi Nagasawa, Ikuko Gomi, Hiroyuki Kugota, Keigo Nanjo, Tetsuya
Jinno, Tadashi Masuda, Sadao Morita. Eﬀects and feasibility of exercise therapy combined with branchedchain amino acid supplementation on muscle strengthening in frail and pre-frail elderly people requiringlong-term care: A crossover trial. Applied Physiology, Nutrition, and Metabolism. 2016.04; 41(1); 438-445
9. Yoshiike T, Nishida M, Yagishita K, Nariai T, Ishii K, Nishikawa T.. Altered Sleep Spindles in Delayed
Encephalopathy after Acute Carbon Monoxide Poisoning. J Clin Sleep Med.. 2016.06; 12(6); 913-915
10. Suzuki H, Ito Y, Shinohara M, Yamashita S, Ichinose S, Kishida A, Oyaizu T, Kayama T, Nakamichi
R, Koda N, Yagishita K, Lotz MK, Okawa A, Asahara H.. Gene targeting of the transcription factor
Mohawk in rats causes heterotopic ossiﬁcation of Achilles tendon via failed tenogenesis. Proceedings of
the National Academy of Sciences of the United States of America. 2016.07; 113(28); 7840-7845
11. Hiroki Katagiri, Kaori Nakamura, Toshifumi Watanabe, Hideyuki Koga, Kazuyoshi Yagishita, Ichiro
Sekiya, Takeshi Muneta. Increase of patellofemoral height has decreased maximum knee ﬂexion after
total knee arthroplasty of posterior cruciate-substituting prosthesis in a clinical series. J Orthop Sci.
2016.07; 21(4); 458-462
12. Fukasawa S, Churei H, Chowdhury RU, Shirako T, Shahrin S, Shrestha A, Wada T, Uo M, Takahashi
H, Ueno T. Application of addition-cured silicone denture relining materials to mouthguard adjustment
Dent Mater J. 2016.07; 35(4); 635-643
13. Junya Aizawa, Shunsuke Ohji, Hideyuki Koga, Tadashi Masuda, Kazuyoshi Yagishita. Correlations between sagittal plane kinematics and landing impact force during single-leg lateral jump-landings. J Phys
Ther Sci. 2016.08; 28(8); 2316-2321
14. Yoko Yamamoto, Yoshihiro Noguchi, Mitsuhiro Enomoto, Kazuyoshi Yagishita, Ken Kitamura. Otologicalcomplications associated with hyperbaric oxygen therapy. Eur Arch Otorhinolaryngol. 2016.09; 273(9);2487-2493
15. Ishigami T, Takahashi T, Kurokawa K, Ueno T. Eﬀects of occlusal supporting area on dynamic postureagainst disturbance by electrical stimulation Int J Sports Dent. 2016.10; 9(1); 7-17
16. Shrestha A, Takahashi T, Kurokawa K, Mitsuyama A, Hayashi K, Ishigami T, Shahrin S, Chowdhury
RU, Churei H, Ueno T. Eﬀects of mouthguards on electromyographic activity of masticatory muscles Int
J Sports Dent. 2016.10; 9(1); 27-37
17. Takahiro Wada, Hiroshi Churei, Toshiaki Ueno, Motohiro Uo. High shock absorbing faceguard usingﬁber-reinforced plastic (FRP) and elastomer The Journal of the Japanese Society for Dental Materials
and Devices. 2016.11; 35(6); 325-328
18. Nakamura T, Yoshida Y, et al. The Eﬀect of Teeth Clenching on Dynamic Balance at Jump-Landing: A
Pilot Study. Journal of Applied Biomechanics. 2016.11; 19; 1-19
19. Toshitaka Yoshii, Kenichiro Sakai, Takashi Hirai, Tsuyoshi Yamada, Hiroyuki Inose, Tsuyoshi Kato,
Mitsuhiro Enomoto, Shoji Tomizawa, Shigenori Kawabata, Yoshiyasu Arai, Atsushi Okawa. Anterior
decompressionwithfusionversusposteriordecompressionwithfusionformassivecervicalossiﬁcationofthe
posteriorlongitudinalligamentwitha≥50%canaloccupyingratio:amulticenterretrospectivestudy.SpineJ.2016.11;16(11);1351-1357
20. Kazuyoshi Yagishita, Tetsuya Jinno, Daisuke Koga, Tsuyoshi Kato, Mitsuhiro Enomoto, Tsuyoshi Kato,
Takeshi Muneta, Atsushi Okawa. Transient osteoporosis of the hip treated with hyperbaric oxygen therapy: a case series. Undersea Hyperb Med. 2016.11; 43(7); 847-854
21. Tomomasa Nakamura, Yuriko Yoshida, Hiroshi Churei, Junya Aizawa, Kenji Hirohata, Takehiro Ohmi,
Shunsuke Ohji, Toshiyuki Takahashi, Mitsuhiro Enomoto, Toshiaki Ueno, Kazuyoshi Yagishita. The Eﬀect
of Teeth Clenching on Dynamic Balance at Jump-Landing: A Pilot Study. J Appl Biomech. 2016.12;
1-19
22. Fukasawa S, Churei H, Chowdhury RU, Shahrin S, Shrestha A, Wada T, Uo M, Takahashi H, Ueno T.
Diﬀerences among shock-absorbing capabilities of mouthguard materials Dent Traumatol. 2016.12; 32(6);474-479― 564 ―

Sports Science Organization

Sports Science Organization

23. Toshitaka Yoshii, Takashi Hirai, Kenichiro Sakai, Shinichi Sotome, Mitsuhiro Enomoto, Tsuyoshi Yamada,
Hiroyuki Inose, Tsuyoshi Kato, Shigenori Kawabata, Atsushi Okawa. Anterior Cervical Corpectomy and
Fusion Using a Synthetic Hydroxyapatite Graft for Ossiﬁcation of the Posterior Longitudinal Ligament.
Orthopedics. 2016.12; 1-6
24. Shinichi Shirasawa, Hideyuki Koga, Masafumi Horie, Tomomasa Nakamura, Toshifumi Watanabe, Ichiro
Sekiya, Takeshi Muneta. Forced knee extension test is a manual test that correlates with the unstablefeelings of patients with ACL injury before and after reconstruction. Knee. 2016.12; 23(6); 1159-1163[Misc]
1. Mitsuhiro Enomoto, Takashi Hirai, Hidetoshi Kaburagi, Takanori Yokota. Eﬃcient Gene Suppressionin Dorsal Root Ganglia and Spinal Cord Using Adeno-Associated Virus Vectors Encoding Short-Hairpin
RNA. Methods Mol. Biol.. 2016; 1364; 277-290
2. Mitsuhiro Enomoto. Therapeutic eﬀects of neurotrophic factors in experimental spinal cord injury models
Journal of Neurorestoratology. 2016; (4); 15-22[Conference Activities & Talks]
1. Ryota Shimamura, Takumi Yamada, Sadaya Misaki, Takehiro Omi, Takanobu Morozumi, Yoshinao Sato,
Nami Shida, Kazutaka Sugimoto. The Gait Analysis of Patients after Bi-Cruciate Retaining TKA. The
46th Annual Meeting of the Japanese Society for Replacement Arthroplasty 2016.02.26
2. Shunsuke Ohji, Junya Aizawa, Kenji Hirohata, Takehiro Ohmi, Tomomasa Nakamura, Kazuyoshi Yagishita. Characterization of vertical and posterior ground reaction force during single leg jump-landing
with body rotation. Société Internationale de Traumatologie et Médicine des Sports d Hiver 2016.03
3. Yusuke Nakagawa, Hideyuki Koga, Takeshi Muneta, Kiuchi S, Ono H, Nagata T, Toshifumi Watanabe,
Masafumi Horie, Tomomasa Nakamura, Koji Otabe, Mai Katakura, Hisako Katano, Ichiro Sekiya. Threedimensional Reconstructed MRI Evaluation of Arthroscopic Centralization Technique for Extruded Lateral
Meniscus. Orthopaedic Research Society 2016 Annual Meeting 2016.03.05
4. Toshifumi Watanabe,Takeshi Muneta, Ichiro Sekiya, Hideyuki Koga, Masafumi Horie, Tomomasa Nakamura, Koji Otabe, Scott Banks. In-vivo Kinematics of Posterior-stabilized Total Knee Prosthesis Designed
for Asian. Orthopaedic Research Society 2016 Annual Meeting 2016.03.05
5. Ikegawa M, Takahashi T, Ueno T. Eﬀects of water gargling on changes of saliva pH and buﬀering capacity
by taking sports drinks during exercise. 7th International Conference of Sports & Society 2016.06.02
Hawaii, U.S.A.
6. Takahiro Wada, Hiroshi Churei, Shintaro Fukasawa, Takahiro Shirako, Toshiaki Ueno, Motohiro Uo.
Shock-absorbing property of face guard by incorporating a glass-ﬁber reinforced plastic and space. 2016.06.12
7. Shirako K, Churei H, Ueno T. Splinting by use of novel luxated tooth model and Periotest. 19th World
Congress on Dental Traumatology 2016.08.11 Brisbane, Australia
8. Akiko Kawashima, Takumi Yamada, Takehiro Omi, Yoshinao Sato, Ryota Shimamura. Hip Joint Reaction Force in the Single Leg Standing and Kneeling Postures. Asian Confederation of Physical Therapy
Congress 2016.10.07 Kuala Lumpur, Malaysia
9. Yoshinao Sato, Takumi Yamada, Takehiro Omi, Ryota Shimamura. Comparison of Foot Kinetics in the
Frontal Plane during Gait Initiation between Young and Elderly Subjects. Asian Confederation of Physical
Therapy Congress 2016.10.07 Kuala Lumpur, Malaysia
10. Takehiro Omi , Takumi Yamada, Yoshinao Satoh Ryota Shimamura. Simulation of the eﬀect of decreasing
lateral component of ﬂoorground reaction force during walking on mechanical stress of knee joint. Asian
Confederation of Physical Therapy Congress 2016.10.07 Kuala Lumpur, Malaysia
11. Takahiro Wada, Hiroshi Churei, Haruka Takayanagi, Mako Yokose, Shitaro Fukasawa, Takahiro Shirako,
Toshiaki Ueno, Naohiko Iwasaki, Hidekazu Takahashi, Motohiro Uo. Development of a faceguard using a
glass-ﬁber reinforced thermoplastic (GFRTP) and shock escape space. Visual-JW 2016 2016.10.17 Suita,
Osaka, Japan― 565 ―

Sports Science Organization

Sports Science Organization

12. Takahiro Wada, Hiroshi Churei, Shintaro Fukasawa, Takahiro Shirako, Toshio Hongo, Toshiaki Ueno,
Motohiro Uo. Analysis of shock absorption ability of a faceguard by incorporating a glass-ﬁber reinforced
thermoplastic and space. International Dental Materials Congress 2016 2016.11.05 Bali, Indonesia
13. Tanabe G, Churei H, Wada T, Uo M, Takahashi H, Ueno T. Eﬀect of the use of commercial cleaning
liquid in thermoforming process of mouthguard materials. International Dental Materials Congress 20162016.11.05 Bali, Indonesia
14. Hidetoshi Kaburagi, Takashi Hirai, Takanori Yokota , Atsushi Okawa,Mitsuhiro Enomoto. Role ofvoltage-gated sodium channels and calcium channel subunit alpha-2/delta-1 in the lumbar dorsal root
ganglions of persistent peripheral neuropathic pain model mice.. Neuroscience2016 2016.11.15 San Diego― 566 ―