Pipette Accuracy Tester
PT-Series
AD-4212B-PT
AD-4212A-PT
FX-300i-PT

INSTRUCTION MANUAL

1WMPD4001794E

© 2015 A&D Company, Limited. All rights reserved.
No part of this publication may be reproduced, transmitted, transcribed, or translated into
any language in any form by any means without the written permission of A&D Company,
Limited.

The contents of this manual and the specifications of the instrument covered by thismanual are subject to change for improvement without notice.

Microsoft, Windows, Excel and Word are either registered trademarks or trademarks ofMicrosoft Corporation in the United States and/or other countries.

CONTENTS
1.
1-1
1-2
1-3
1-4
1-5

INTRODUCTION.............................................................................................................. 2
Unpacking the Tester...................................................................................................... 2
Description of Each Component ..................................................................................... 3
WinCT-Pipette, Software for Pipette Accuracy Testing.................................................... 4
Function of the Evaporation Trap.................................................................................... 5
Description of a Sample Cup .......................................................................................... 5

2.

ASSEMBLING THE TESTER ........................................................................................... 6
2-1 AD-4212B-PT / AD-4212A-PT ........................................................................................ 6
2-2 FX-300i-PT..................................................................................................................... 8

3.

PRECAUTIONS BEFORE USE...................................................................................... 10

4.

ANTI-STATIC TREATMENT ............................................................................................11

5.

INSTALLING WinCT-Pipette............................................................................................11

6.

CONNECTING A PC TO THE BALANCE ....................................................................... 12
6-1 Connecting a PC .......................................................................................................... 12
6-2 Checking COM Port Numbers ...................................................................................... 13

7.

CALIBRATING THE BALANCE ...................................................................................... 14
7-1 AD-4212B-PT / AD-4212A-PT ...................................................................................... 14
7-2 FX-300i-PT................................................................................................................... 15

8.

PREPARATION BEFORE USE ...................................................................................... 16
8-1 Filling the Evaporation Trap with Water ........................................................................ 16
8-2 Preparing the Test Liquid .............................................................................................. 16
8-3 Preparing the Water-absorbent Sheet .......................................................................... 16
8-4 Using the Thermometer................................................................................................ 17

9.

MEASUREMENT ........................................................................................................... 23
9-1 Measuring Procedure ................................................................................................... 23
9-2 Judging the Results...................................................................................................... 23
9-3 Example Causes of Measurement Errors ..................................................................... 23

10. AD-4212B-PT / AD-4212A-PT ........................................................................................ 25
10-1 Factory Settings ........................................................................................................... 25
10-2 Display Lock Function .................................................................................................. 26
10-3 Auto Print Mode............................................................................................................ 26
11.

TROUBLESHOOTING ................................................................................................... 27

12. SPECIFICATIONS.......................................................................................................... 28
12-1 Options......................................................................................................................... 29
13.

APPENDIX ..................................................................................................................... 30

11. INTRODUCTION
The PT series Pipette Accuracy Tester performs an accuracy test of the volume of a pipette in accordance with
ISO 8655.
This manual describes how the PT series Pipette Accuracy Tester works and how to get the most out of it interms of performance. It includes how to assemble the tester, how to measure and how to use the software,
WinCT-Pipette, for pipette accuracy testing.
Read this manual thoroughly before using the tester and keep it at hand for future reference.
For detailed information on handling the balance, refer to the instruction manual for the balance used.
NOTE

ISO 8655 is the international standard that includes pipette calibration and specifies a test
method for accuracy of the pipette volume. For detailed information, visit our web site.

1-1 Unpacking the Tester
NOTE

The pipette accuracy tester is contained in a carrying case. The case is only for carrying
the tester and is not solid enough to endure transportation. So, do not transport thetester using the carrying case. When transporting the tester, be sure to use the original
packing material.
When handling the tester, be careful not to drop it, even if it is placed in the carrying case.

The illustrations below show what the PT series consists of. Note that the USB converter is shipped in a
separate package, but it can be placed in the carrying case as shown below.
A shoulder belt and a key are provided with the carrying case. Use the shoulder belt to carry the case.
NOTE

Please confirm that the AC adapter type is correct for your local voltage and power
receptacle type.

AD-4212B-PT / AD-4212A-PT
The connection cable provided as an accessory is one meter long.

2

FX-300i-PT

1-2 Description of Each Component
 Evaporation trap
A chamber to prevent the evaporation of the dispensed test liquid (distilled water).
(Refer to “1-4 Function of the Evaporation Trap”.)
 Sample cup (Two types: 5 mL and 30 mL)
A measuring cup to contain the test liquid dispensed from a pipette, with awater-absorbent sheet installed, to prevent water droplets from remaining on theedge of the pipette tip.
 Test liquid cup
A container to keep a test liquid such as distilled water.
 Thermometer (with a clamp)
Measures the temperature of the test liquid.
 RS-232C cable and USB converter
Connects to a PC (personal computer) to transmit the test values to the PC.
 WinCT-Pipette
Software for pipette accuracy testing to convert the test liquid mass into a
volumetric value.
 Calibration weight (with a pair of tweezers to handle the weight)
A weight to calibrate the balance.

31-3 WinCT-Pipette, Software for Pipette Accuracy Testing
Features
The mass value of the distilled water dispensed from a pipette is transmitted from the balance to a PC.
Using the temperature of the test liquid and the barometric pressure that are previously entered, the PC
calculates the conversion factor (Z factor) from a mass value to a volumetric value and converts themass value into a volumetric value automatically, using the Z factor.
The measurement results are compared with the specifications previously entered, to perform
judgment whether the pipette will pass or fail.
Besides the pipette volume, test numbers, test dates and pipette information (manufacturer, model,
serial number) can be recorded as test data. The test data, output to a printer or stored in the PC,
allows easy management of pipette accuracy and reduces errors due to data mismanagement.
The specifications of accuracy and repeatability can be entered either as an absolute value (L) or
as a relative value (percentage to the pipette volume).
The mass value data from the balance can be transmitted to the PC either using a COM port orusing a USB connection. (For a USB connection, use the accessory USB converter.)For testing various pipettes easily, multiple test conditions (pipette volume, number ofmeasurements and specifications) can be set previously. When multiple operators are set
previously, an operator can be selected easily as necessary.
NOTE

For detailed information on WinCT-Pipette, refer to “How to use WinCT-Pipette.pdf” in the
WinCT-Pipette CD-ROM.

Main Window
When WinCT-Pipette starts up, the window below opens
Specifications
Enter the pipette volume, specificationsof accuracy and repeatability used
for pass/fail judgment.
Measured Values
Displays the mass values transmitted fromthe balance and the volumetric values
obtained using the Z factor.
Measurement Results
Displays the measurement results
and judgment results.

Measurement environment
Enter the ambient relative humidity,
the distilled water temperature andthe barometric pressure.
The Z factor is automatically calculated using the values of the
distilled water temperature and the barometric pressure.

4

1-4 Function of the Evaporation Trap
In the pipette accuracy test using the gravimetric method, to obtain the mass of the test liquid, distilled water
is dispensed from the pipette into the sample cup installed on the balance. The obtained value, however, isgenerally smaller due to evaporation loss of the test liquid. To prevent evaporation of the test liquid, theevaporation trap is used. The ISO 8655 standard recommends that the humidity of the measurement
environment be greater than 50% RH.
Water is poured into the groove of the evaporation trap. This helps to maintain the humidity inside theevaporation trap high and reduces evaporation. Using the evaporation trap reduces the evaporation of
the test liquid, compared with when the evaporation trap is not used. This yields a measurement
environment where there is no need to consider measurement errors due to evaporation loss of the test
liquid.
Another function of the evaporation trap is one as a breeze break, to prevent drafts in the measurement
environment from influencing the weighing operation and enable a stable weighing.
The evaporation trap is designed for an easy dispensing of the test liquid. The upper part has a sloped
surface so that the pipette can be inserted from obliquely above.

1-5 Description of a Sample Cup
When dispensing a test liquid from the pipette, to perform an accuracy test of the volume of a pipette, apossible cause of errors is water droplets remaining on the edge of the pipette tip. Using thewater-absorbent sheet in the sample cup prevents this and increases the accuracy of a test.
As shown in the illustration below, a water-absorbent sheet is rolled into a cylinder and is placed along
the inner wall of the sample cup.
By dispensing the test liquid with the edge of the pipette tip touching the sheet, the sheet will absorb
any droplet which may otherwise remain on the edge of the tip. Thus all the test liquid will be dispensed
into the sample cup.
The water-absorbent sheets are made of a RoHS-compliant material for safety.
The sheet, once used, can be cleaned and used repetitively.

52. ASSEMBLING THE TESTER
2-1 AD-4212B-PT / AD-4212A-PT
Display

1. Place the weighing unit on a solid table. For details

Connection
cableon the installation site, refer to “3. PRECAUTIONSBEFORE USE”.
Secure the display to the stand using the angle
adjustment knobs. Connect the display and theWeighing
unit

Stand

Angle
adjustment
knob

weighing unit using the connection cable.
Evaporation
trap
base
2. Install the evaporation trap base on the weighing unit.

3. Install the sample cup holder (30 mL) on the base.

5 mL sample
cup

When the 30 mL sample cup is used:
Insert the sample cup into the holder.
NOTE

Sample cup
holder (5 mL)

The actual capacity of the 30 mL
sample cup is 25 mL.

Sample cup
holder (30 mL)

When the 5 mL sample cup is used:
Insert the sample cup holder (5 mL) into thesample cup holder (30 mL), and then insert
the sample cup

4. Install the lower part of the evaporation trap on

Lower part ofthe evaporation trap

the weighing unit, mating the tabs with theholes on the base.

Tab
Hole

6

30 mL sample
cup

5. Attach the upper part of the evaporation trap to thelower part, mating the tab with the notch.

Upper part ofthe evaporation trap
Tab
Lower part ofthe evaporation trap

Notch

6. Adjust the direction of the evaporation trap so that the pipette insertion opening on the sloped
surface is faced to allow easy operation.

Pipetteinsertion
opening

Facing to the rightof the weighing unit
右Facing to the leftof the weighing unit
左Pipetteinsertion
opening

Cap B

7. Install cap B on the evaporation trap.
Remove cap A from the sloped surface.

Upper part ofthe evaporation trap

7

Cap A

2-2 FX-300i-PT
1. Place the balance on a solid table.
For details on the installation site, refer to “3.
PRECAUTIONS BEFORE USE”.
.

2. Install the fitting boss on the balance, then install the
Evaporation
trap
base

evaporation trap base on the fitting boss.

Fittingboss

3. Install the sample cup holder (30 mL) on the base.
5 mL sample
cup

When the 30 mL sample cup is used:
Insert the sample cup into the holder.
NOTE

The actual capacity of the 30 mL sample
cup is 25 mL.

Sample cup
holder (5 mL)

When the 5 mL sample cup is used:
Sample cup
holder (30 mL)

Insert the sample cup holder (5 mL) into thesample cup holder (30 mL), and then insert
the sample cup

8

30 mL samplecup

4. Install the lower part of the evaporation trap on
the weighing unit, mating the tabs with the holes

Lower part ofthe evaporation trapon the base.
Tab
Hole

5. Attach the upper part of the evaporation trap toUpper part ofthe evaporation trap

the lower part, mating the tab with the notch.

Tab
Lower part ofthe evaporation trap

Notch

6. Adjust the direction of the evaporation trap so that the pipette insertion opening on the sloped
surface is faced to allow an easy operation.

Pipetteinsertion
opening

Facing to the rightof the balance

右Facing to the leftof the balance

左Pipetteinsertion
opening

Cap B

7. Install cap B on the evaporation trap.
Remove cap A from the sloped surface.

Upper part ofthe evaporation trap

9

Cap A

3. PRECAUTIONS BEFORE USE
To get the optimum performance from the balance and acquire accurate weighing data, note thefollowing, especially when using the AD-4212B-PT / AD-4212A-PT with a minimum weighing value of0.001 mg / 0.1 mg.
Install the balance where the measurement environment is appropriate. The best operating
temperature is 15°C to 30°C ±0.5°C with greater than 50% RH.
Install the balance where it is not exposed to direct sunlight and it is not affected by air fromheaters or air conditioners. Especially be careful when using the AD-4212B-PT. It responds even tovery subtle airflow.
Install the balance where it is free of dust.
Install the balance away from equipment which produces magnetic fields.
Install the balance in a stable place avoiding vibration and shock. Corners of rooms (close to pillars
or walls) far from a passage way are best, as they are less prone to vibration.
The weighing table should be solid and free from vibration, drafts and as level as possible.
Level the balance by adjusting the leveling feet and confirm it using the bubble spirit level.
Before use, warm up the balance for the appropriate duration, with nothing on the weighing pan.
AD-4212B-PT / AD-4212A-PT: one hour or more
FX-300i-PT: 30 minutes or more
When using the AD-4212B-PT with a minimum weighing value of 0.001 mg, keep the balance
connected to power constantly.
Calibrate the balance before use or after having moved it to another location. Use the accessory
calibration weight for calibration. For details, refer to “7. CALIBRATING THE BALANCE”.

Caution
Do not install the balance where flammable or corrosive gas is present.

10

4. ANTI-STATIC TREATMENT
An anti-static treatment has been applied to the evaporation trap. Cleaning it with alcohol will reduce
the effect of the treatment.
If the evaporation trap has acquired a static charge, apply an anti-static agent or use the optional
AD-1683 DC static eliminator to remove the static electricity.

5. INSTALLING WinCT-Pipette
For detailed information on the WinCT-Pipette program, refer to “How to use WinCT-Pipette.pdf” in the
WinCT-Pipette CD-ROM.
For installing WinCT-Pipette, refer to “Readme.txt” in the WinCT-Pipette CD-ROM.
System requirements
OS
CPU
RAM
Hard disk available space
Display

Windows XP SP2 or later
Pentium or the equivalent, 1GHz or more recommended
512 MB or more recommended
Approximately 50 MB
1024 x 768 or more recommended

116. CONNECTING A PC TO THE BALANCE
To make a connection between a PC and the balance, use the RS-232C cable or use both the
RS-232C cable and the USB converter.

6-1 Connecting a PC
When a PC COM port is used
When using the COM port of a PC to make a connection to the balance, use the accessory RS-232C
cable.

AD-4212B-PT / AD-4212A-PT

FX-300i-PT

When a PC USB port is used
When using the USB port of a PC to make a connection to the balance, use both the accessory
RS-232C cable and the USB converter (shipped in a separate package).
The USB converter, when connected to the USB port of a PC, adds a COM port to the PC. Whenconnecting the USB converter to the USB port for the first time, installing a driver is required. (SomePCs may require a driver installation even when the USB port used is changed.)
The driver installation CD and the instruction manual are contained in the same package with the USB
converter. For instructions on installation, read the manual.
After driver installation, connect the USB converter to the balance, using the RS-232C cable. Now a COM
port is added and communications between the PC and the balance is enabled.

NOTE

Although the USB converter is shipped in a separate package, it can be placed in the carryingcase.
AC adapter jackRS-232C interface

USB converter

AC adapter jackRS-232C cable

RS-232C interface
RS-232C
cable
USB converter

Weighing unit
connection jack

Personal computer

Personal computer

AD-4212B-PT / AD-4212A-PT

FX-300i-PT

12

6-2 Checking COM Port Numbers
COM port numbers vary depending on the PC used. Use the Windows Device Manager to check COM port
numbers.

NOTE

When the USB converter is used, check the COM port number with the USB converter
connected.

1 Click [Start], [Settings] and [Control Panel].
2 Double-click [System].
3 Click [Hardware] and [Device Manager].
4 Double-click [Port (COM & LPT)]. COM port numbers will be displayed.
When the USB converter is used, “ATEN USB to Serial Bridge (COMx)” appears.
In the example below, the COM port number is 4.
The COM port for a personal computer is displayed as “Communications Port (COMx)”.
In the example below, the personal computer has two COM ports. Therefore, two COM ports
appear; “Communications Port (COM1)” and “Communications Port (COM2)”
Change the WinCT-Pipette COM port number to that of the COM port used.

Checking COM port numbers in [Device Manager]137. CALIBRATING THE BALANCE
For details on calibration, read the balance instruction manual in addition to this manual.
Before calibration, refer to “3. PRECAUTIONS BEFORE USE”. Especially when calibrating theAD-4212B-PT / AD-4212A-PT with a minimum weighing value of 0.001 mg / 0.1 mg, make sure that the
installation environment is appropriate and the balance is warmed up for the appropriate duration.

7-1 AD-4212B-PT / AD-4212A-PT
1. Remove the sample cup, but leave two sample
cup holders (5 mL and 30 mL) inside theSample cup
holder (5 mL)

evaporation trap, as shown to the right.

Upper part ofthe evaporation trap

Lower part ofthe evaporation trap
2. Press and hold the CAL key until

Caloutisdisplayed, then release the CAL key.
3. When

Cal 0

is displayed, press the

Sample cup
holder (30 mL)

PRINT key to enter the zero point.

Tab

4. After the zero point is entered, the calibration
weight value

50

is displayed.

Remove the upper part of the evaporation trap
while holding the lower part of the evaporation

Calibration
weight (50 g)

trap.
Place the accessory calibration weight on thesample cup holder (5 mL).
Attach the upper part of the evaporation trap
to the lower part, mating the tab with thenotch.

5. Press the PRINT key to calibrate the weighing
capacity. Whenend

is displayed, remove

the calibration weight. The balance will returnto the weighing mode.
6. Place the calibration weight on the sample

cup holder (5 mL) to confirm that calibration
has been completed successfully.

14

Notch

7-2 FX-300i-PT
Upper part ofthe evaporation trap

1. Remove the 30 mL sample cup and leave only
the sample cup holder (30 mL) inside theevaporation trap as shown to the right.

2. Press and hold the CAL key until

CaloutLower part ofthe evaporation trapisSample cup
holder (30 mL)

displayed, then release the CAL key.
3. When

Cal 0

is displayed, press the

PRINT key to enter the zero point.

Tab(4 in all)

4. After the zero point is entered, the calibration
weight value

100

is displayed.

Calibration
weight (100 g)

Remove the evaporation trap by holding thelower part of the evaporation trap.
Place the accessory calibration weight on thesample cup holder (30 mL).

Base

Attach the evaporation trap on the base,Hole(4 in all)mating the tabs with the holes.

5. Press the PRINT key to calibrate the weighing
capacity. Whenend

is displayed, remove

the calibration weight. The balance will returnto the weighing mode.
6. Place the calibration weight on the sample

cup holder (30 mL) to confirm that calibration
has been completed successfully.

NOTE

The evaporation trap can be used as a breeze break.
Calibration can be performed, using the weighing pan and the breeze break provided asaccessories.

158. PREPARATION BEFORE USE
The measurement environment recommended by ISO 8655 is as follows.
Temperature: Constant between 15°C and 30°C, changes in temperature ±0.5°C.
Humidity: Greater than 50% RH

8-1 Filling the Evaporation Trap with Water
Remove the evaporation trap from the balance. Separate the upper and lower part of the evaporation trap.
Pour water into the groove along the circumference of the inner wall of the lower part of the evaporation trap,
using a wash bottle or a pipette, up to the center between the two lines on the evaporation trap.
Wash bottle
NOTE When pouring water, use much care notto spill water around the fitting boss ofthe balance weighing pan.
Lower part ofthe evaporation trap

Water levelreference lines

Attach the upper part of the evaporation trap to the lower part and place the evaporation trap on thebalance or weighing unit.
It takes approximately 15 minutes for the humidity inside the evaporation trap to stabilize to about 85% RH. The
time required for the humidity to stabilize varies depending on the measurement environment.

8-2 Preparing the Test Liquid
Pour distilled water into the test liquid cup.
Place the thermometer in the cup, using the clamp as shown below.
Leave as is to allow the temperature of the distilled water to stabilize. ISO 8655 recommends that thetest liquid be acclimatized to the measurement environment for two hours or more.

Thermometer

Clamp

Insert the thermometer
into the cup so that theclamp is hooked on theedge of the cup.

Test liquid cup

8-3 Preparing the Water-absorbent Sheet
To stabilize the evaporation amount of the dispensed test liquid from the sheet, moisten the sheet withthe test liquid (approx. 600L for the 5-mL sample cup sheet, and approx. 4000L for the 30-mL sample
cup sheet) before the test.

16

8-4 Using the Thermometer
Each part name
LCD

Battery cover[ON/OFF] button[AUTO OFF] button[MAX/MINI] button

Sheath holder

SheathMaterial: Main body, battery cover: Acrylonitrile-butadiene- styrene (ABS)
Button: Rubber
Display: Polycarbonate (PC)
Sheath: SUS304
Sheath holder: Polycarbonate (PC)
Sheath holder fitting: Iron (Nickel plating)
Display

Auto power off function

Maximum value display
Minimum value display

Measurement temperature display(Maximum value /Minimum value display)

Low battery indicator

Error displayloHiAppears when the measurement temperature is below the lower limit for the display range,or there is an error in the temperature sensor or internal circuit.
Appears when the measurement temperature exceeds the upper limit for the display range,or there is an error in the temperature sensor or internal circuit.

17

Replacing The Battery
The battery is not installed when you purchase the thermometer. Properly install the battery in thethermometer by the following procedure.
Also, when the “” indicator appears at the bottom right in the display, replace the old battery with a

new one by the following procedure.
* Purchase the battery at your local convenience store, supermarket, hardware store or electrical store.
* When the main body is damp, wipe the moisture fully and replace the battery.
The thermometer may be damaged if water gets inside.
1. Turn the battery cover on the rear side of the main body in counterclockwise direction by using a
coin, and remove the battery cover.
2. Remove the old battery.
3. Place a new CR2032 coin type lithium battery with the + side facing up.
4. Place the battery cover back on.
As shown in the figure below, place the battery cover so that the mark on the battery cover is alignedwith the upper mark on the main body. Then, turn the battery cover in clockwise direction using acoin until the mark on the battery cover is aligned with the lower mark on the main body.
The battery cover that is not secured properly may result in entering water inside and damaging thethermometer.

OP
EN

CL
OS
E

CLOSE
OPEN

CAUTION
Precautions on the battery use
 The provided battery is for monitoring purposes, and its life may therefore be shorter thanthe battery life specified in this manual.
 Remove the battery from the thermometer and keep it elsewhere when you are not using thethermometer for a long period of time.
 Use the specified battery (CR2032 coin type lithium battery 1 pc) only.
 Be sure to insert the battery with the + side facing up. Reverse polarity may not only cause
operation errors but also damage the thermometer.
 Do not recharge the battery, short-circuit, disassemble or thrown into the fire. It may
explode or leak and cause personal injury.
 Keep the battery out of the reach of children. If swallowed, consult a physician immediately.
 For environmental protection, follow the local regulations to dispose of a used battery.

18

Measuring the temperature
1. Press the [ON/OFF] button to turn the power on.
2. Remove the sheath holder when it is attached to the sheath.
3. Measure the temperature by one of the following methods
depending on the material to be measured.

When measuring the liquid
Immerse the sensor into the position where you would like tomeasure the temperature.
When there is steam arising from the liquid, handle thethermometer so that the display is not exposed to the steam.
Otherwise the steam may damage the thermometer.

When measuring the semisolid material
Stick the sensor to the depth where you would like to measure thetemperature in the material.
Attempting to forcibly stick the sensor into the material when themeasurement material is too solid may damage the sheath. In
such case, a device that enables smooth insertion of the sensor
is required.

19

When measuring the gas
Place the sensor in the location where you would like to measure the temperature and wait until thedisplay becomes stable.
When measuring a high temperature atmosphere such as steam, use a shield to protect the display
from high temperature.
Gas

ShieldCAUTION
The thermometer detects a temperature at the tip of the sheath.
To protect the thermometer from damage, do not expose parts other than the sheath to highor low temperatures of the substances to be measured.
SheathTemperature detection part

Storing the maximum and minimum temperatures in memory
The thermometer can automatically store the maximum

Currently
measuredtemperatureand minimum temperatures measured after the battery is
installed in the thermometer (or after the memory is reset).
Press thePress the [MAX/MIN] button to display the maximum or[MAX/MIN] button

minimum temperature as shown on the right.

Maximum
temperature
display

The thermometer automatically displays the current
measurement temperature if no operation is made for 30

Press the [MAX/MIN]

seconds while on the maximum or minimum temperature

buttondisplay

Minimum
temperature
display

To check the maximum and minimum temperatures for a
new measurement, reset the memory before starting thePress the [MAX/MIN]

measurement as described in “Resetting the maximum andbuttonminimum temperatures stored in memory” below.

20

CAUTION
The maximum and minimum temperatures stored in memory are reset automatically when
the battery is removed from the thermometer.

Resetting the maximum and minimum temperatures stored in memory
To reset the maximum and minimum temperatures stored inmemory, perform the following:℃ ＭAX

Press the [MAX/MIN] button to display the maximum or minimum
temperature. Press the [MAX/MIN] button again and hold it for
more than two seconds. The display goes into the bar display, andboth the maximum and minimum temperatures stored in memory
are reset to store new maximum and minimum temperatures.

About the auto power off function and turning the power off
Auto power off function℃ AUTO

The thermometer has the auto power off function.
The auto power off function is disabled when the battery has been
replaced.
To enable the auto power off function, press the [AUTO OFF]button one time. The “AUTO” appears at the upper right in thedisplay to show that the auto power off function is now enabled.
With the auto power off function, the thermometer automaticallyturns the power off when no operation is made for about fiveminutes. To disable the auto power off function, press the [AUTO
OFF] button again.
The “AUTO” at the upper right in the display disappears, and theauto power off function becomes disabled.

Turning the power off
To turn the power off immediately, press the [ON/OFF] button.

21

How to use the sheath holder
Use the sheath holder to protect the sheath when you carry the thermometer.
Be sure the sheath holder is removed from the sheath when performing measurements.
To prevent the sheath holder from deformation or damage, before using the sheath holder, allow the
sheath to return to room temperature after high or low temperature measurements.

Daily careAccurate measurements cannot be made if the temperature detection part is dirty. It should be kept
clean when performing measurements.
Do not rub the main body to clean. Rinse the dirt off using water.
When the dirt is hard to remove, wipe it off gently using a kitchen sponge, etc., dampened with a neutral
detergent.
Do not use abrasives or volatile solvents such as thinner or benzine.

Specifications
Sensor: Thermistor

Temperature measurement range: -40.0°C to +260.0°C

Display resolution: 0.1°C

Measurement accuracy: ±1.0°C (-9.9 to 59.9°C)±2.0°C (-19.9 to -10.0°C, 60.0 to 99.9°C)±3.0°C (-29.9 to -20.0°C, 100.0 to 199.9°C)±4.0°C (-40.0 to -30.0°C, 200.0 to 260.0°C)

Display refresh rate

: Every second

Waterproof level: JIS IPX7 (Waterproof for 30 minutes at a water depth of 1 meter, still waterat room temperature)

Power supply: CR2032 x 1 pc

Battery life

: Approx.1 year (When used for one hour per day)Operating temperature and humidity : 0 to 40°C, 75%RH or less, No condensation (Excluding the sheath)
Storage temperature and humidity: 0 to 50°C, 75%RH or less, No condensation

External dimensions of the sheath : 2.8 mm (Tip: 2.2 mm, length 20 mm)
Approx. 120 mm long
External dimensions: 40(W) x 187(H) x 17(D) mm (Excluding the protrusion)

Weight: Approx. 27 g (Including the battery and sheath holder)Standard accessories: Instruction manual, sheath holder, battery (For monitoring purposes)

229. MEASUREMENT
9-1 Measuring Procedure
Using the predetermined pipette volume, perform the measurement the specified times.
ISO 8655 recommends ten measurements for each for the volumes described below.
 At the nominal volume of a fixed volume pipette At the following three points of an adjustable volume pipette
1. 10% of the maximum volume or the lower limit of the adjustable volume range, whichever is greater.
2. 50% of the maximum volume
3. 100% of the maximum volume
NOTE

Pipette volumes and number of measurements can be changed.

9-2 Judging the Results
WinCT-Pipette converts the mass values into volumetric values, compares these with thepredetermined judgment standard and performs judgment whether the pipette will pass or fail.
 Judgment standard
The averaged value (exceeds / does not exceed) the specified maximum permissible error.
Repeatability (exceeds / does not exceed) the specified maximum permissible error.

9-3 Example Causes of Measurement Errors
Evaporation of the test liquid
Evaporation of the distilled water dispensed from the pipette into the sample cup can be a cause ofmeasurement error.
To reduce errors caused by evaporation
Be sure to use the evaporation trap. Using the evaporation trap, evaporation of the test liquid can be
reduced to 0.05 mg per minute (0.05 L per minute).
With the AD-4212B-PT / AD-4212A-PT, use the display lock function. For details, refer to “10-2Display Lock Function”.

Water temperature and barometric pressure
Correctness of the Z factor, that is used to convert a mass value to a volumetric value, depends on thedensity by the water temperature.
The barometric pressure, even if changed in the range between 950 hPa and 1050 hPa, influences
measurements only by 0.01%.
Water temperature, if changed by 5°C, influences measurements by 0.11%.

23

To reduce errors caused by water temperature
Use the correct Z factor. To obtain the correct Z factor, measure the water temperature using theaccessory thermometer and enter the value in WinCT-Pipette.
For a stable water temperature, allow the temperature of the test liquid to acclimatize to themeasurement environment for an appropriate duration of time (ISO 8655 recommends two hours ormore) before measurement.

Vibration
Vibration yields unstable measurement values.
To reduce errors caused by vibration
Install the balance in a stable place avoiding vibration and shock. Use a solid weighing table that is
free from vibration.
If the installation site is the second floor or higher, use the AD-1685 anti-vibration table.

Airflow
Airflow in the measurement environment yields unstable measurement values
To reduce errors caused by airflow
Install the balance where it is not affected by heaters or air conditioners or where no drafts exist.
Especially when using the AD-4212B-PT / AD-4212A-PT with a minimum weighing value of 0.001 mg /0.1 mg, use much care in choosing an installation site or cover the weighing unit.

Operator
An operator’s pipetting technique influences measurements.
To reduce errors caused by the pipetting techniqueLearn and use the correct pipette technique.

2410. AD-4212B-PT / AD-4212A-PT
The AD-4212B-PT / AD-4212A-PT is designed exclusively as a pipette accuracy tester.
Although the performance as a balance is the same as the AD-4212B / AD-4212A production weighing
unit, some of the specifications are different.

10-1 Factory Settings
When shipped, the factory settings of the AD-4212B-PT / AD-4212A-PT as a pipette accuracy tester are
as follows.

Unit Weighing unit: mg
 Minimum weighing value for the AD-4212B-PT: 0.1 mg
Each time the SAMPLE key is pressed, the minimum weighing value changes as below:
0.1 mg  0.01 mg  0.001 mg  1 mg  0.1 mg 
Press the SAMPLE key to switch the minimum weighing value as necessary.
For testing a liquid with a volume of less than 100 L, the recommended minimum weighing value is0.01 mg (0.01 L). With a volume of less than 10 L, 0.001 mg (0.001 L).

Function table
Functions related to pipette accuracy testing are as follows.
Class

Item and Parameter

Cond
Condition
trc
ba5fncEnvironment Zero tracking
Display
5pd
Display refresh rate
ploc
Display lock function

Cp fncComparator

Cp
Comparator mode

Description
Fast weighing speed, sensitive value

0
FAST
1
MID.
2 Slow weighing speed, stable value
SLOW
0 OFF
1 Normal
2 Strong
3 Very strong
0 5 times/second
1 10 times/second
0 OFF
1 ON
0 No comparison
1 Comparison, excluding “near zero” when stable or overloaded
2 Comparison, including “near zero” when stable or overloaded
3 Continuous comparison, excluding “near zero”
4 Continuous comparison, including “near zero”
Factory setting

25

10-2 Display Lock Function
To use this function, set “Display lock function (ploc)” to “ON (1)”.

Why use this function
This function is used to compensate time-elapsed errors caused by evaporation.
Even when the evaporation trap is used, an evaporation of 0.05 mg per minute (0.05 L per minute) will
occur. This evaporation influences the measurement, particularly when the volume of the test liquid issmall. For such a situation, the display lock function is useful.

How this function works
After the test liquid is dispensed from the pipette, measurement starts. When the mass value is stable,
data is averaged. After a certain period of time, the averaged value is locked in the display.
Processing indicator
Stabilization indicator
IlluminatedProcessing indicator
Blinking

Averaging process
NOTE

Locked display

The RE-ZERO operation unlocks the display.

10-3 Auto Print ModeIn the balance function setting of “Data output (dout)”, when “Data output mode (prt)” is set to “Autoprint mode A (1)”, the mass value will be transmitted to WinCT-Pipette automatically once the mass
value of the dispensed test liquid becomes stable.

2611. TROUBLESHOOTING Displayed values are unstable.
The AD-4212B-PT / AD-4212A-PT has a minimum weighing value of 0.001 mg / 0.1 mg and is
influenced by the measurement environment.
Measures to take Use the accessory breeze break.
 Referring to “3. PRECAUTIONS BEFORE USE”, check the measurement environment for sourcesof drafts, vibration and static electricity.
 The AD-4212B-PT with a minimum weighing value of 0.001 mg is prone to low-frequency vibrationsuch as shaking buildings. Particularly, earthquake, wind and changes in the barometric pressure
influence measurement. To avoid vibration, install the balance on the first floor, in the corners ofrooms (close to pillars or walls) far from a passage way.
 Use the AD-1685 anti-vibration table.
 Use the optional AD-1683 DC static eliminator or use the optional AD-1684 electrostatic field meter
if the cause of the unstable values may be due to static charges.
 Repeatability of the measurement values is low. The measurement values are not correct.
Measures to take
 Referring to “3. PRECAUTIONS BEFORE USE”, check the measurement environment. Using theaccessory calibration weight, measure the balance repeatability and check the balance
performance.
 Referring to “9-3 Example Causes of Measurement Errors”, check the measurement environment
for causes of errors.

 Balance error displays
Refer to the balance instruction manual.

27

12. SPECIFICATIONS
AD-4212B-PT

AD-4212A-PT

FX-300i-PT

Weighing capacity *1

110 g / 31 g / 5.1 g *2

110 g

320 g

Minimum weighing
value

0.1 mg / 0.01 mg / 0.001 mg

0.1 mg

1 mg

Linearity±0.2 mg / ±0.05 mg / ±0.05 mg±0.3 mg±2 mg

Repeatability(Standard deviation)

0.1 mg / 0.05 mg / 0.015 mg

0.15 mg

1 mg

Dimensions

Weighing unit: 80 (W) x 230 (D) x 200 (H) mm
Display (with a stand): 237 (W) x 150 (D) x 155 (H) mm

193 (W) x 262.5 (D)x190 (H) mm

Standard accessories  Instruction manual*3 Balance including the weighing pan unit, breeze break, AC adapter and AC
adapter ID label
 Calibration weight with a pair of tweezers
 Evaporation trap Sample cup (30 mL x 2 / 5 mL x 2) *4
 Sample cup holder (30 mL / 5 mL)
 Thermometer USB communications kit (USB converter, RS-232C cable, Instruction
manual) WinCT-Pipette (CD-ROM)
 Carrying case with a shoulder belt and a key
AC adapter

Please confirm that the AC adapter type is correct for your local voltage andpower receptacle type.

Pr consumption

Approx. 11VA (supplied to the AC adapter)

Carrying casedimensions

470 (W) x 150 (D) x 355 (H) mm

Mass (With allaccessories in a case)Approx. 7.6 kg

Approx. 7.2 kg

Approx. 6.4 kg*1 When the balance weighing pan is used.
*2 The AD-4212B-PT is equipped with a smart range function. When the minimum weighing value isset to 0.001 mg and the mass value exceeds 5.1 g, the minimum weighing value will switch to 0.01
mg, and when the mass value exceeds 31 g, the minimum weighing value will switch to 0.1 mg
automatically. Even under this circumstance, pressing the RE-ZERO key tares the value andweighing with the minimum weighing value of 0.001 mg is available from that point up to 5.1 g.

*3 The standard accessories for the AD-4212B-PT / AD-4212A-PT / FX-300i-PT are different fromthose for the AD-4212B / AD-4212A / FX-300i.

*4 Sample cups and water-absorbent sheets are available separately for purchase as options.

28

12-1 Options
Name and components
5-mL sample cup (with a water-absorbent sheet installed)
5-mL sample cup
5 pcs
Lid for 5-mL sample cup
5 pcs
Water-absorbent sheet for 5-mL sample cup
5 pcs(The sheet is installed in the cup before shipping.)
30-mL sample cup (with a water-absorbent sheet installed)
30-mL sample cup
5 pcs
Lid for 30-mL sample cup
5 pcs
Water-absorbent sheet for 30-mL sample cup
5 pcs(The sheet is installed in the cup before shipping.)
Water-absorbent sheet for the 5-mL sample cup
5 pcs
Water-absorbent sheet for the 30-mL sample cup
5 pcs

29

Model

AX-PT-01

AX-PT-02

AX-PT-03
AX-PT-04

13. APPENDIX
The table below lists the relation between the ISO 8655 requirements and the PT series pipette accuracy
testers.
ISO 8655 requirements

Pipettenominalvolume*5

Maximum permissible error
Accuracy

Repeatability

(L)

±%

±L

%

L

1

5.0

0.05

5

0.05

2

4.0

0.08

2

0.04

5

2.5

0.125

1.5

0.075

10

1.2

0.12

0.8

0.08

20

1.0

0.2

0.5

0.1

50

1.0

0.5

0.4

0.2

100

0.8

0.8

0.3

0.3

200

0.8

1.6

0.3

0.6

500

0.8

4.0

0.3

1.5

1000

0.8

8.0

0.3

3.0

2000

0.8

16

0.3

6.0

5000

0.8

40

0.3

15

10000

0.6

60

0.3

30

Balance minimum
weighing value
mg

0.001

Pipette accuracy tester
corresponding to the requirements

0.01

The AD-4212B-PT *6

0.1

The AD-4212A-PT

Daily inspection, simplified verification*7

FX-300i-PT*5 The maximum volume selectable for variable volume pipettes*6 The AD-4212B-PT can be used for the pipette volume range from 1 L to 10000 L.
We recommend using the following balance with the BM-014 to ensure measurement stability andprecision in the range that a minimum weighing value is 0.001 mg.
BM-20 or BM-22 : Micro analytical balance.
BM-014 : Pipette accuracy tester kit for the BM series to measure the pipette volume.
*7 The minimum weighing value, 1 mg, approximately corresponds to 1 L. If a pipette volume is 1000 L, atest can be performed with a resolution of 0.1%. If 200 L, 0.5%.
NOTE Pipette accuracy testing is performed using a high-resolution balance. Make sure that themeasurement environment is free from vibration, drafts and air from air conditioners. For
details, refer to “3. PRECAUTIONS BEFORE USE”.

30

MEMO

31

MEMO

32

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