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cell invasion by down-regulating Smad2 expression inhuman gastric cancer. Vitexin(2',4'-dihydroxy-6'-methoxychalcone) is a type offlavonoid isolated from the plant Vitex negundo, which
has been demonstrated to have anti-cancer effects. The
purpose of the present study was to evaluate themolecular mechanism by which vitexin exerts its anticancer activity in human gastric cancer. The effect ofvitexin treatment on the invasion of gastric cancer cells
was determined using Matrigel invasion assays. The
levels of Smad2 phosphorylation and protein
expression were measured by Western blotting. The
Smad2 gene promoter activity and Smad2 mRNA
expression were analyzed by luciferase reporter assays
and real-time PCR, respectively. The effect of Smad2
overexpression on vitexin-induced cell invasion wasalso analyzed. Vitexin inhibited the invasion of gastriccancer cells by down-regulating the expression of
Smad2 and Smad2 phosphorylation. Overexpression of
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