MCE USA
Tel:609-228-6898

Email: tech@MedChemExpress.com

Compound Handling Instructions

1

What are the shipping conditions of MCE products?

A: MCE stable products are shipped at room temperature.
B: Liquid or special products are shipped with blue ice or MCE® Cooling Rack in afoam holding box. MCE products are relatively stable at room temperature. Theirquality will not be affected if the blue ice melts upon receiving, and products canto tech@MedChemExpress.com. If you can not find the solubility information
you are looking for, or you are having difficulty preparing a product in solution
form, you can also get help via the email above. Once prepared, aliquot the stock
solution to routine usage volumes and store at -20°C or -80°C. Avoid repeatedfreeze and thaw cycles.

MCE is dedicated to provide high quality products and meet customer
satisfaction. If you have any questions or concerns, please contact after-sales
staff on phone 609-228-6898 or by email sales@MedChemExpress.com.

Samplebe used with confidence.
DMSO > A mg/mL

DMSO soluble (A mg can be dissolved in 1 mL
DMSO, saturation unknown)

H2O < B mg/mL
DMSO < C mg/mL

H2O insoluble or slightly soluble
DMSO insoluble or slightly soluble (C mg can notbe dissolved in 1 mL DMSO)

2How can I obtain product Data Sheet, QA ﬁles, etc.?

A: We automatically send product Data Sheet and QA documents to your email
address after shipment.

Molarity Calculator from MCE official website is recommended for thecalculation: www.MedChemExpress.com/Molarity%20Calculator.htm

B: You can download these files on the product webpage of our websiteMedChemExpress.com.
C: You can also request the materials from our customer service.

6How do I dilute the compound solution for cell assay?

Stock solution using ddH2O as a solvent can be directly diluted with medium toprepare the working solution.

3

What are the proper storage guidelines for MCE product?

When DMSO is used to prepare the stock solution, the stock solution is diluted in
the culture medium to prepare a working solution. Make sure the concentration

Recommended storage conditions and precautions regarding proper productof DMSO is <0.5% to avoid poisoning the cells. A negative control in thehandling are contained in the product Certificate of Analysis (COA).

experiment is usually the culture medium with DMSO at the same concentration.

Here are GENERAL storage guidelines for compounds:

It is recommended that the process of dilution is performed in a stepwise

Powder:In solvent:-20°C

3 years

4°C

2 years-80°C

6 months-20°C

1 month

If the solution is stored at -20°C for more than one month, it should be
re-examined to ensure its efficacy. Avoid repeated freeze and thaw cycles.
Storage conditions for some special products should refer to their COAs.

manner to avoid compound precipitating caused by fast change ofconcentration.

7

How do I use these compounds during animal experiments? ie:method of administration, dosage, solvent preparation, andadministrative cycle?

Stock solution using ddH2O as a solvent can be directly diluted with PBS or 0.9%
NaCl to ready the working solution.

4

How should I handle the products before formulation or use?

During transportation, the compounds may adhere to the neck or cap of the vial.
Before opening the vial, please centrifuge to gather the compound at the bottomof the vial.

Stock solution using DMSO as a solvent can also be diluted with PBS or 0.9%
NaCl to prepare the working solution. In order to reduce its toxicity to animals, thefinal concentration of DMSO in working solution should preferably be 2% or lower.
When precipitates form during the dilution process due to their low watersolubility, you can also use a co-solvent to help dissolve the compounds.
Commonco-solvents

containglycerol,

Tween

80,

sodiumcarboxymethylcellulose (CMC-Na), cyclodextrin, PEG400, etc. A suspension can65

How can I prepare the compound stock solution?

Select the appropriate solvent for the preparation of stock solution based on youralso be used for oral or intraperitoneal injection. Please send an email totech@MedChemExpress.com if you require further assistance.

experiment needs.

Methods of administration and solvent preparation used in publications may be

Solubility information is available at the product webpage. Currently we only offer

available at the product webpage. MCE has not independently confirmed thesolubility data in DMSO and/or water, for solubility of other solvents, please email

accuracy of these methods and they are distributed for reference only.

Conversion between different animal models based on BSA:

Administration volumes considered good practice (and possible maximal
dose volumes):Species

Weight (kg)Body Surface Area (m2)

Km factor

Dog

10

0.5

20

Rabbit

1.8

0.15

12

Guinea pig

0.4

0.05

8Rat

0.15

0.025

6Hamster

0.08

0.02

5

Mouse

0.02

0.007

3

Animal A (mg/kg) = Animal B (mg/kg) multiplied by68

Route and volumes (mL/kg)
Species

Orals.c.

i.p.

i.m.

i.v. (bolus)

i.v. (slow inj.)

Mouse

10 (50)10 (40)20 (80)0.05 (0.1)

5(25)

Rat

10 (40)5 (10)

10 (20)0.1 (0.2)

5(20)Rabbit10 (15)1 (2)5 (20)

0.25 (0.5)

2(10)

Dog

5 (15)1 (2)

1 (20)

0.25 (0.5)

2.5

(5)

Animal B Km

Products are for research use only and are not intended for human use.

Animal A Km

We do not sell to patients.

Is the compound sterile?

DMSO or water is commonly used to prepare compound solutions. If sterilization is needed, it is recommended to filter the working solution through a 0.22 μm sterile
filter membrane. Before filtering, please ensure that both the stock solution and working solution are clear and free of precipitation under microscopic observation.
Avoid using high-temperature and high-pressure sterilization methods.
1. If DMSO is used as the solvent, the prepared solution is generally sterile due to DMSO's antimicrobial properties. However, it is important to keep the operating
environment and the instrument sterile. It is recommended to prepare a 1,000× DMSO stock solution before the experiment. If contamination occurs, filter the working
solution for sterilization.
2. If water is used as the solvent, it is recommended to prepare a stock solution of 50× or higher with sterile water, and then filter the working solution for sterilization.

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