PlantNo1

AmaranthCharacters

Plant: time of519
No. of samples
Block

Primary essential character

Methods
Observation

Rank or measurement unit

Remarks
Observed at inflorescence emergence stage: 50 %date

beginning of emergenceof plants ear over 1 cmof inflorescence2

Plant: time ofBlock

Observation

Observed at flowering stage: 40 to 50 % ofdate

flowering

plants begin to bloom (checked by the existenceof anther and pollen)

3

Plant: time of maturity Block

Observation

Observed at mature stage: 50 % of plants

date

produce mature seeds
4

Stem: color

Block

Observation

3:Green5

Leaf blade: color

Block

Observation

1:Light green
7:Red

6

Inflorescence: color

Block

Observation

3:Yellow
8:Brown

7

Inflorescence: type

Block

Observation

1:Amarantiform

8

Inflorescence: shape

Block

Observation

1:Drooping

9

Inflorescence: length

10 plants

Measurement

cm (integer)

4:Yellow

5:Pink3:Medium green

4:Green

7:Purple

Observed at full flowering stage

5:Dark green

Observed at full flowering stage

6:Red

5:Pink

6:Red

7:Purple

Observed at full flowering stage

9:Glomerulate

3:Spreading

5:UprightObserved at full flowering stage

7:Other

Observed at mature stage
Inflorescence length from top to flag leaf inmain stem at mature stage

10

Plant: length

10 plants

Measurement

Measured length of plant from base to top at

cm (integer)

mature stage
11

Seed: color

Block

Observation

1:White3:Yellow

5:Pink

7:Brown

9:Black

Observed at mature stage

PlantNo
1AmaranthCharacters

519
No. of samples

Cotyledon: intensity of Block

Primary optional character

Methods

Rank or measurement unit

Remarks

Observation

1:Absent

3:Weak5:Medium

7:StrongObserved within 3 to 6 days after emergence

Observation

1:Absent

3:Weak5:Medium

7:Strong

Observed within 3 to 6 days after emergence

Observation

datereddish coloration2

Hypocotyl: intensity of Block
reddish coloration

3

Plant: time of harvest

Block(Leafy amaranth) Observed at harvest stage:for vegetable

plant height reaches approximately 25 cm

4Young leaf: length

10 plants

Measurement

cm (roundto the 1st decimal place)

Measured on the young plant with 6 to 8 leaves

5

Young leaf: width

10 plants

Measurement

cm (roundto the 1st decimal place)

Measured on the young plant with 6 to 8 leaves

6Young leaf: ratio

10 plants

Measurement

3:Small

7:Large

Measured on the young plant with 6 to 8 leaves

Observation

3:In middle towards base 5:Moderately towards
base 7:Strongly towards baseObserved on the young plant with 6 to 8 leaves

Block

Observation

3:WeakObserved on the young plant with 6 to 8 leaves

Block

Observation

1:Light green 3:Medium green
7:Red 9:Purple

Block

Observation

3:Colored basal area 5:Central blotch
7:Colored margin and veins

Observed on the young plant with 6 to 8 leaves

Block

Observation

3:Green

5:Red

Observed on the young plant with 6 to 8 leaves

BlockObservation

1:Entire

9:Sinuate

5:Medium

length/width
7

Young leaf: position of Blockwidest point

8

Young leaf: prominence

5:Medium

7:Strongof veins
9Young leaf: main color
on upper side

10

Young leaf:
distribution of

5:Dark green

Observed on the young plant with 6 to 8 leaves

secondary color on
upper side
11

Young leaf: color on

7:Purplethe lower side
12

Leaf: margin

Observed at vegetative stage, just before
inflorescence emergencePlant

Amaranth519

Primary optional character

NoCharacters

13

Stem: color of stripes

Block

Observation

1:Absent

3:Light green

14

Petiole: intensity ofBlock

Observation

1:Absent

3:WeakLeaf blade: presence of Block

Observation

1:Absent

9:Present

Block

Observation

1:Green

3:Silvery

Block

Observation

1:Chimaera 2:Vein 3:Center 4:Margin
shaped 6:Entire 7:Amorphous

18

Inflorescence: attitude Block

Observation

1:Upright 3:Weakly recurved 5:Moderately
recurved 7:Strongly recurved

Observed at full flowering stage

19

Inflorescence: length

Block

Observation3:Shorter

Observed at mature stage

10 plants

Measurement

Number per plant (roundplace)

No. of samples

Methods

Rank or measurement unit
5:Red

5:Medium

Remarks
Observed at full flowering stage

7:Purple

Observed at full flowering stage

7:Strong

reddish coloration
15

Observed at full flowering stage

blotch16

Leaf blade: color of

5:Red

Observed at full flowering stage

7:Purpleblotch17

Leaf blade:distribution of blotch

5:Equal5:V-

7:Longer

Observed at full flowering stageof bract relative toutricle
20

Plant: number ofbranches per main stemto the 1st decimal

Number of first lateral branches (over 30 cm
length with leaves and flowers) on mein stem.
Measured at mature stage

21

Stem: size

10 plants

Measurement

mm (roundto the 1st decimal place)

Measured size of stem on the base at maturestage

22

Seed: shape

Block

Observation

1:Ellipsoid23

Seed: perisperm type10 grains

Measurement

1:Non-glutinous

9:Discoid
9:Glutinous

Observed on dry seeds at harvest time
Observed by reaction to potassium iodide
solution24

Seed: weight per 1000
seeds

Block

Measurementg (roundto the 1st decimal place)Calculated by measuring the 100 seeds atharvest timePlantAmaranth519

Secondary essential character

NoCharacters

1

Lodging tolerance

Block

Observation

3:Weak5:Medium

7:Strong

Observed at mature stage

2

Insect resistance

Block

Observation

3:Weak5:Medium

7:Strong

Observed through a cultivation period (recordNo. of samples

Methods

Rank or measurement unit

Remarks

insect name as far as you know)
3

Mosaic disease

Block

Observation

3:Weak5:Medium

7:Strong

resistance4Bacterial wilt

Extent of mosaic-like symptoms on leaf surface.
Observed through a period of time

Block

Observation

3:Weak5:Medium

7:Strong

Observed at early growth stage

Block

Observation

3:Weak5:Medium

7:Strong

Observed until vegetative stage, just before

resistance5

Stem rot resistanceinflorescence emergencePlantNo
1

Amaranth
Characters

Cold resistance for519
No. of samples
Block

Secondary optional character

Methods
Observation

Rank or measurement unit
3:Weak5:Medium

7:Strong

vegetable

Remarks(Leafy amaranth) Cold torelance is evaluated byseed germination and seedling growth tests
under low temparature2

Hot resistance forBlockObservation

3:Weak5:Medium

7:Strongvegetable
3

Viviparity(Leafy amaranth) Heat torelance is evaluated byseedling growth test under high temparature

BlockObservation

3:High5:Medium

7:Low

Observed at harvest timePlantNo
1

Amaranth
Characters

Seed yield519
No. of samples
Block

Tertiary essential character

Methods
Measurement

Rank or measurement unit
kg/a (roundto the 1st decimal place)

Remarks
Weight of natural dried seed per unit ares (1are)

2

Glossiness of grain

BlockObservation

1:Glossy

9:Powdery

Observed by transillumination survey method(Glossy: transmissive, Powdery: nontransmissive)PlantNo

AmaranthCharacters

519
No. of samples

Tertiary optional characterMethods

Rank or measurement unit

1

GBSSI genotype

Measurement

1:Functional

2Amylose content inMeasurement% (roundMeasurement

mg/100g f.w. (round

9:Non-functional

to the 1st decimal place)

Remarks
Determined by genotyping
Measured by autoanalyzer, etc.

grains3

Ascorbic acid content

to the 1st decimal place)

Measured by High-performance liquid
chromatography (HPLC), reflectometer (such asRQflex), etc.

4

Oxalic acid content

Measurement

mg/g d.w. (roundto the 1st decimal place)

Measured by High-performance liquid
chromatography (HPLC) , etc.

5

Rutin content

Measurement

mg/g d.w. (roundto the 1st decimal place)

Measured by High-performance liquid
chromatography (HPLC) , etc.

*6

Sucrose content

Block

Measurement

mg/g d.w. (roundto the 1st decimal place)

Measured by High-performance liquid
chromatography (HPLC) , etc.

*7

Glucose content

Block

Measurement

mg/g d.w. (roundto the 1st decimal place)

Measured by High-performance liquid
chromatography (HPLC) , etc.

*8Fructose content

Block

Measurement

mg/g d.w. (roundto the 1st decimal place)

Measured by High-performance liquid
chromatography (HPLC) , etc.

*9Total polyphenol

Block

Measurement

mg/g d.w. (roundto the 1st decimal place)

Block

Measurement

mol Trolox/g (round

Measured by Folin-Ciocalteu method, etc

content*10 Antioxidant activity

to the 1st decimal place)

Measured by DPPH free radical scavenging assay,etc* Unlisted item in Genebank Descriptor