Test Compounds

ReportRepeatReviseSimple, Safe Dosing
Research Diets, Inc. will incorporate your compounds into any
experimental diet. Feeding test compounds eliminates dosing
related stress to the animal, eliminates vehicle effects, and saves
time and labor. Consult with one of our scientists on the formula,determine the dosage required and the diet will be produced andshipped in 5 to 7 business days.

Repeat Formula and Dose Response
Consistent OpenSource Diet® formulation provides a clean,
repeatable control diet for your research. Precise, graded additionof test compounds to your specified control diet allows evaluationof dose response effects in your animal model. We can blend yourcompound homogeneously into any diet, typically to ppm andeven as low as parts per billion.

Examples of Compounds Added• Chemotherapeutics• COX-2 Inhibitors• Antioxidants• Nutraceuticals• Statins• Insulin sensitizers• NSAIDS

A Dose Response Study

Pelleted Micro Batches

30 mg/kg

Research Diets will incorporate test compounds into pelleted
micro batches as small as 200 grams for those compounds thatare in limited supply. Our production facility is well equippedto pellet small batches at precise doses.

20 mg/kg
10 mg/kg
0 mg/kg

We start by
thoroughly mixing
the compound in asmall bowl with apremix and dye.

How to Calculate the Diet Dose of your Compound

Then we transfer
the contents of thesmall bowl into alarger bowl.

The dye assures thatthe compound ishomogeneously
mixed and readyfor pelleting.

STEP 1: VARIABLES NEEDED

Variables

Units

Single Daily DoseSD =mg Cmpd/kg BW/day

Body WeightBW =gm BW/animal

Daily Food Intake

FI =gm Diet/day

Diet Dose

DD=mg Cmpd/kg Diet

STEP 2: PLUG FIGURES INTO FORMULA

Formula: DD= (SD X BW)/FITest Compounds
Pica Behavior

Contact our Resource Center for valuable insight from more than
25 years of product experience incorporating test compounds intodiet. Let us formulate the diets to meet your specific study needs.
Custom OpenSource Diets
Research Diets, Inc has pioneered the incorporation oftest compounds into pelleted purified diets.
Our scientists specialize in providing custom purified
OpenSource diets. By carefully designing the diet formula
to fit your protocol, you have complete control over smallor large changes in diet composition. Plus you are able toreport what your animals were fed, repeat the formulaand revise diet composition as necessary.

Visceral illness is an undesirable side effect of some pharmaceuticaltreatments and experimental paradigms, and confounds the study ofappetite regulation. By definition, visceral illness describes a range ofnausea and concomitant anorexia caused by many factors, including
ingestion of toxins, infections, presence of cancer, etc. Consumptionof non-nutritive substances (pica behavior) such as kaolin, has been
documented to reflect feelings of visceral illness in rats and mice.

Kaolin Pellets

Value Added Resource
The value of our products is in the scientific support weprovide. Our Resource Center is staffed with Masters andPh.D. level scientists with access to over 14,000 original
formulas and a database of more than 3,800 journal
articles. We welcome the opportunity to talk science withresearchers throughout the world as we maintain ourleadership role as the knowledge base for OpenSource
Diet formulation.

BioDAQ Episodic Intake Monitor
BioDAQ E2 Episodic monitoring system measures thead libitum food and water intake behavior of singly
housed lab rats and mice at very high resolution in theirhome cage. Computer controlled electronics record foodand water intake episodically by measuring the moment to-moment, undisturbed intake behavior of the animals
being studied. The animal’s behavior defines the variable
period for these intake measurements through theirnative behavior.

Where NutriPhenomics® Begins

Kaolin Use in Research• Pre-clinical Drug Discovery• Obesity and diabetes• Oncology• Radiation Therapy• Chemotherapeutics• Nausea research• Anti-emetics• Cachexia

Cost Effective Indicator
Research Diets, Inc. offers, a non-nutritive kaolin pellet for use inyour research. It is a cost effective, easy to use, early indicator of
visceral illness in your experimental animals. Early identificationof this adverse experience profile of a compound saves money andstreamlines the rational drug design process. Researchers studying
metabolic disorders rely on food intake measures as a primary
endpoint in evaluating the efficacy of experimental treatments.
However, anorectic effects can reflect primary efficacy or secondary
effects due to visceral illness. Consumption of kaolin offers early
insight into the mechanisms operating in your in vivo studies.

Research Diets, Inc.
20 Jules Lane
New Brunswick, NJ 08901 USA
Tel: 732.247.2390
Fax: 732.247.2340
info@researchdiets.com© 2009 Research Diets, Inc. All rights reserved. TC-3000-2-09