Table of Contents

1. Foreword

1		
2. History
2-1. Establishment Background 

22-2. Laboratory Transition 

22-3. Publications and Grants

3

3. Management
3-1. Basic Policies on Institute Management

43-2. Research Exchange Promotion

6

3-3. Diversity and Inclusion

9

4. Education
4-1. Graduate Education

11

4-2. Training the Next Generation of Researchers

12

5. Research
5-1. Laboratory Introductions 

14

6. Comments from the Reviewer Board
6-1. Yingzi Yang (Harvard Univ., USA)

44

6-2. Akihiko Yoshimura (Keio Univ., Japan) 46

6-3. Injune Kim (KAIST, Korea) 48

7. Discussion and Future Prospects
7-1. Toshio Suda (Former Director)

49

7-2. Hitoshi Takizawa (Current Director)

50

8. Appendix
8-1. List of Staff and Students

51

8-2. List of Graduates

53

8-3. List of Interns

548-4. List of Publications

56

8-5. List of Grants

76

8-6. List of Seminars & Symposia

778-7. List of Visiting Researchers

91

8-8. List of Advisory Board

93

9.Editorial Note 941. Foreword

Foreword     I am happy to announce that herewith we have issued a report with an overview of thevision and identity of International Research Center for Medical Sciences (IRCMS) in Kumamoto
University, Japan. This report provides a summary of a number of past and current activities andaccomplishments that all the researchers within the research center have worked on together, for the
first 9 years since its establishment in 2015. IRCMS has introduced a new research system with two
unique features, a transparent and effective governance and an international research environment
under the leadership of the director. The former has enabled a new personnel system in Japan
including annual salaries and cross-appointment which are distinguishable from those existing in theJapanese university, while the latter has enabled English-based organization and operation both inscience and administration, including recruitment of international students/PIs, core facility, open lab
system and facilitating collaborations with overseas institutions.
The target research field of IRCMS has been stem cell, development, aging, and cancer, in orderto understand the basic principle of somatic stem cell biology and its application to medicine: blood
stem cells and their potential to reconstitute blood and immune system; vascular cell development
and its metabolism to treat adult disease; brain stem cell development and brain disorders; skinstem cell homeostasis and aging toward anti-aging/rejuvenation therapy; endothelial-mesenchymaltransition to cancer and developing a measure to prevent/treat cancer. Along with the researchdevelopment, IRCMS has also developed its own training programs, such as an internship program
to recruit students, a fellowship to support graduate students, lecture series (“science and me”, “nextgeneration PI seminar”), social events such as happy hour, public events, etc.
With the above-mentioned efforts that have been made for the last 9 years, I am confident
that IRCMS has successfully established an internationally recognized research center in the fieldof stem cell and blood research, and fostering young
researchers for future science. I hope that we canfurther grow and expand our science hand in hand with
our peers, and that our progress and achievements
could help to open a new door to biology and medicine
in the future.
Director, International Center for Medical Sciences
Kumamoto University
Hitoshi Takizawa―1―2. History
2-1. Establishment Background
The Aim of International Research Center for Medical Sciences (IRCMS)
IRCMS is an organization established in 2015 in conjunction with the “University Strategy Council”. Upon its
establishment, the following was identified as roles that the IRCMS should fulfill:- Oversee research organizations in the field of life sciences at Kumamoto University.
- Conduct international cutting-edge research.
- Promote international collaborative research.
- Foster and reconstruct life science research hubs.
- Identify and nurture leading young talents.
- Extend world-class distinctive research and sharpen new research areas.
- Promote integrated research and clinical studies beyond departmental boundaries to enhance functionality.
The establishment process of the IRCMS
In 2013, Professor Masafumi Takiguchi, then the head of the AIDS Research Center at Kumamoto University, took
the initiative to establish a new international research institute that conducts cutting-edge medical research. His
proposal was approved by the university headquarters, marking the beginning of the IRCMS. In March 2014, a fivestory research building was completed in the Honjo-Naka campus. Professor Toshio Suda (National University ofSingapore) was designated as the inaugural director of the center.
On January 15, 2015, a kickoff symposium was held, where world-renowned researchers in areas of stem cells,
AIDS, and cancer delivered lectures. In the symposium, attendants discussed which direction IRCMS should go asa research institute.
Starting from April 2015, four young Principal Investigators (PIs) from the AIDS Research Center moved in, andresearch activities in IRCMS officially began. Additionally, at that time, President Isao Taniguchi granted ten-year
positions to three PIs. Recruits for these positions included Goro Sashida, Hitoshi Takizawa, and Guojun Sheng.
Laboratory activities commenced with a focus on AIDS studies and stem cell research.
In 2016, a similar organization, the International Research Organization for Science and Technology (IROAST)
was established in the Kurokami campus to develop an international hub in the field of engineering research. Both
IRCMS and IROAST initiated organizational exchanges to achieve the common goal of internationalization, holding
frequent joint meetings and collaborations.
In 2023, Hitoshi Takizawa succeeded Toshio Suda as the director of IRCMS.

2-2. Laboratory Transition
Current Status:
IRCMS has surpassed the initial expectations and has developed well as an international research center. It currently
comprises 12 Principal Investigators (PIs) and approximately 80 researchers, 40% of whom being foreign nationals
as of March 2023. The center2015 2016 2017 2018 2019 2020 2021 2022 2023 2024
(Relocation)
has played an advanced role in Suda Lab.
(The University of Miyazaki, Japan)
Nishiyama Lab.
various aspects of Kumamoto Baba Lab.
(Faculty of Life Science, Kumamoto University)
Sashida Lab.
University’s institutional reforms.
Takizawa Lab.
(National University of Singapore)
Osato Lab.
However, the following issues have
Sheng Lab.
arisen: One of the challenges lies
(Japanese Foundation for Cancer Research)
Ishimoto Lab.
Mizuno Lab.
in transitioning from a “temporary”
Umemoto Lab.
(Kyushu University, Japan)
organization with the 10-year
Sada Lab.
Arima Lab.
term limit to a more sustainable
Kurotaki Lab.
Sato Lab.
Miharada Lab.
o r g a n i z a t i o n a l m o d e l , w h i l e Suzu Lab.
Kurihara Lab.
(Joint Research Center for
Ueno Lab.
solidifying financial foundation.
Takahashi Lab.
Human Retrovirus Infection
Ariumi Lab.
Kumamoto University)

Hanke Lab.
Rowland-Jones Lab.
Appay Lab.
Schoenbach Lab.

―2―

(Astana University, Kazakhstan)2-3. Publications and Grants
Among the missions that IRCMS was given upon its establishment, conducting international cutting-edge research,promoting international collaborative research, and striving for the advancement of world-class research made asignificant advancement. Therefore, in order to report internationally recognized researches, each laboratory madesubstantial efforts, resulting in the following achievements between 2015 and 2023:
Papers published from IRCMS: 306
Among them, international co-authored papers: 188 (61%)
Top 10% papers: 62 (20%)*Criteria:
Search by PI name on Web of Science (2015-2023)
Confirm the search results with the PI to ensure they are IRCMS achievements.
Remove duplicate papers.

External funds obtained by IRCMS
External Funds obtained by IRCMS (2016 - 2023)
500,000,000
400,000,000
300,000,000
200,000,000
100,000,000
0FY 2016

FY 2016
直接経費（円）Direct expense (JPY)
間接経費（円）Indirect expense (JPY)
合計 （円）
Total(JPY)2016年度との比較（％）

Compared to FY2016 (%)

FY 2017

FY 2017

FY 2018

FY 2019

FY 2020

FY 2021

直接経費（円）

間接経費（円）Direct expense (JPY)

Indirect expense (JPY)FY 2018

FY 2019

FY 2020

FY 2021

FY 2022

FY 2023

FY 2022

FY 2023

125,546,156 162,676,142 212,327,051 195,263,692 217,493,678 267,737,049 379,908,922 332,661,694

32,863,844

40,257,459

36,446,501

46,036,108

38,788,846

46,827,000

77,307,029

55,456,708

158,410,000 202,933,601 248,773,552 241,299,800 256,282,524 314,564,049 457,215,951 388,118,402

N/A

128.1

157.0

―3―

152.3

161.8

198.6

288.6

191.3

3. Management
3-1. Basic Policies on Institute Management
Managements and operations are discussed in monthly PI meeting, and PIs and administrative staff work together toachieve the set goal.

Administrative Office (2015-)
The Administrative Office provides personnel, finance, facility,
and research support to all IRCMS researchers, to let them focus
on their research activities without being burdened by extra
administrative work. In contrast to the traditional Japanese lab
system, so-called KOUZA (courses), where each lab has theirself-funded secretaries, our Laboratory Support Service hasa centralized system in which one Laboratory Support Officer
is responsible for 3-4 laboratories on average and assists
administrative procedures associated with research.
The IRCMS also provides any supports in English, the official
language of the center, and offers life support in Japan forresearchers and students coming from abroad, including visa
applications, travel to Japan, finding accommodation, resident
registration, opening a bank account, and finding day care for
their children, to create an international research environment.
With this support, in addition to the Core Facility and theOpen-Lab system, we have created an environment in which
researchers arriving from overseas can start their researchsmoothly and effectively.
Core Facility (2017-)
The International Core-facility of Advanced Life Sciences (ICALS) was established in 2017 with funding from the
Ministry of Education, Culture, Sports, Science and Technology for the purpose of sharing research equipment
between departments in Kumamoto University.
To facilitate collaborative research, ICALS provides research support including mass cytometry, cell sorting,molecular biology technical support, electron microscopy sample preparation, and intravital multiphoton microscopy.
Activity summary1.) Joint research
The shared equipment was used in 35 joint research projects both domestically and internationally, which
contributed to publishing 13 papers.
2.) Contract research
The shared equipment was used in more than 400 contract research experiments. As a result, there wasincome of over 16 million yen in equipment usage revenue.

―4―3.) Others
To promote the sharing of equipment, we have hosted 40 technical seminars and training sessions.
Members         	Hidenobu Mizuno PhD (Meeting chair, general management, microscope management)
Daisuke Kurotaki PhD (Server management)
Yosuke Tanaka PhD (Cell sorter management)
Akifumi Kiyota PhD (Common equipment management, contract research)
Natsumi Miharada PhD (Seminars and training sessions arrangement, inventory management)
PI meeting: PIs gather once a month to share information on and discuss ongoing and future institutional/university
operations as well as future direction that IRCMS envisions. The meeting materials are prepared in Japanese andEnglish, covering topics submitted by the attendees. Discussions focus on recent research progress in papers
and grant acquisitions, ongoing paper preparation as well as potential collaborations within IRCMS and with otherinstitutions.
Personnel: Young researchers who are proactive in their respective research area and motivated for science arerecruited with a fixed-term and annual salary. The candidates are reviewed by the center director, IRCMS professors,and professors in related fields within the university, in terms of the innovative and original elements of the previous
and future research as well as international recognition. Researchers at the postdoctoral level or above undergo anannual performance review, and their salaries are assessed based on the evaluation.
Visiting positions:
In addition to the Advisory Committee, visiting full and associate professors from overseas are appointed as adjunctfaculty members to facilitate international collaborative research and acquire research grants of international funding
bodies.
Lab space, office and data management:
All laboratories are arranged in an open space area, and office spaceis also shared to promote scientific discussion/collaborations. Alllarge equipment is primarily managed and operated through the Core
Facility, and data management is done online through transfer from theequippment directly to an internal server installed in IRCMS. The data
can be analyzed either in the researchers’ PC or computing serverwhenever needed.
Research activities:
Beside independent research done by Principal Investigators (PIs), postdoctoral researchers, and graduate
students, institutional seminars and symposia are organized in English on a monthly basis, by IRCMS researchersand distinguished speakers around world both on site and online.
★ A technician was awarded the Commendation of the Minister of Education, Culture, Sports, Scienceand Technology (MEXT)
Ms. Keida Takako, a technician, was selected and commended
by MEXT as a person of distinguished service in the field of
medical education ( 医学教育等関係業務功労者表彰 ) in 2023.
This award recognizes distinguished service in education andresearch related to medicine and dentistry at national, public,
and private universities, as well as in work related to patientcare. She has worked as a medical technologist and technicalassistant at Kumamoto University for more than 20 years, andwas honored for her long and outstanding contributions. Herlife story can be found in the Nature (“Peer-to-peer careers advice for research managers: Six technicians andmanagers share tips on how their roles can be better developed and supported.” Vol. 606, 16 June 2022, 609-611).
―5―

Research Alliances:
Collaborating and forming alliances with other research institutions and industries can help increase resource
sharing and research efficiency, eventually improving financial sustainability and research outcome. Strengthening
collaboration with the industry through industry-academia partnerships can also promote research projects andtechnology transfers that generate revenue, thereby strengthen the financial foundation.
Public Relations:
IRCMS also focuses on public relations, actively spreading information on IRCMS activities in English and Japanesethrough our official website and various social media (Facebook, X, YouTube, Instagram, and LinkedIn). These
outlets play a vital role in making IRCMS activities visible from the world to elevate our presence.

3-2. Research Exchange Promotion
Collaboration with Clinical Departments of Kumamoto University Hospital:
To promote translational researches, IRCMS recruited Dr Takatsugu Ishimoto from the Department of Gastrointestinal
Surgery at Kumamoto University Hospital, Dr Koichi Nishiyama from the Department of Circulatory Medicine, andDr Yuichiro Arima (current vice director) as Principal Investigators (PIs), propeling research in cancer and vascularbiology.
Collaboration with IROAST of Kumamoto University:
As mentioned in the section of IRCMS history, The International Advanced Science and Technology Research
Organization (IROAST), the IRCMS counterpart in the Faculty of Advanced Science and Technology, was set up
in 2016 following IRCMS establishment. Since then, IRCMS and IROAST have aimed to foster interdisciplinary
collaborative research, conducting regular joint symposia. Significant research outcomes, notably in the fields
of imaging and sheet CT, have been achieved. Additionally, two proposals from Principal Investigators (Arimaand Takizawa) have been funded by the interdisciplinary program, called the Frontier Project, led by Kumamoto
University, which aims to develop novel domains of fusion science. Capitalizing on the foundation of interdisciplinary
research cultivated thus far, IRCMS will continue to strive for the promotion of collaborative efforts both within andoutside the university.
★ IRCMS-IROAST collaborative research (PI: Hidenobu Mizuno)
Dr. Mizuno has been conducting joint research with Dr. Higaki of IROAST (currently a professor at the Facultyof Science) for five years, on data extraction from microscopic images. Their research interests span a wide
range of fields, including stem cell biology, neuroscience, and botanical science. Some of their works cameto fruition as research papers (Higaki and Mizuno, 2020; Takihara, Higaki et al., 2022).

The results were published in Kumamoto University’s magazine (Kumadai-Tsushin Vol.83, 2023) to contributeto improving the university’s outreach activities.

―6―

Publications:
Four-dimensional imaging with virtual reality to quantitatively explore jigsaw puzzle-like morphogenesis of
Arabidopsis cotyledon pavement cells
Higaki T and Mizuno H
Plant Biotechnology 20(4) 429-435 (2020)
Bone marrow imaging reveals the migration dynamics of neonatal hematopoietic stem cells
Takihara Y, Higaki T, Yokomizo T, Umemoto T, Ariyoshi K, Hashimoto M, Sezaki M, Takizawa H, Inoue T, Suda
T and Mizuno H
Communications Biology 5(1) 776 (2022)
International collaborations:PIs involved in international activities have invited renowned overseas researchers to give seminars at IRCMS. Our
history of international exchanges began with collaborative AIDS research with the University of Oxford. In terms of
medical-engineering collaboration, joint symposia with KAIST (Korea Advanced Institute of Science and Technology)
have been alternately held in Daejeon (South Korea) and Kumamoto (Japan), taking place together with a retreat. In
2018, the proposal from IRCMS was selected for the Core-to-Core Program with support from the Japan Society for
the Promotion of Science (JSPS). This led to enhanced international exchanges in stem cell research with institutions
such as the University of Cambridge, Lund University, the University of Zurich, and the University of Cincinnati.
Specific activities supported by this program included research meetings, short stays for young researchers, andattendance at international conferences.
Collaboration with KAIST (Korea)
KAIST is a prestigious research institute in Daejeon, South Korea. Since 2000, Drs Gou Young Koh and Toshio
Suda have collaborated in stem cells and vascular biology. This collaboration has developed into the University
collaboration between KAIST and Kumamoto University under Memorandum of Understanding. Since 2018, we
have been hosting the KU-KAIST Joint Symposium alternatively in Japan and South Korea. Due to the impact ofthe pandemic, in-person events became difficult, but we adapted by organizing an online seminar series featuringprominent researchers from abroad. Especially with strong support of KAIST’s Professors Pilhan Kim and Injune Kim,
our relationship with KAIST has become robust, and several international collaborative research projects have beenlaunched. We aim to further deepen our joint research with KAIST and continue to play a role as an international hub
in the future.

The first KU-KAIST Joint Symposium washeld in KAIST, Daejeon, South Korea, from
October 21 to October 23, 2018. Hitoshi
Takizawa served as the organizer from
Kumamoto University, with several researchers
participating from both IRCMS and IROAST.

2nd KU-KAIST Joint Symposium-4th IRCMS & IROAST Joint
Seminar Jan. 25, 2019―7―

JSPS Core-to-Core Program
In the JSPS Core-to-Core program “Integrative approach for normal and leukemic stem cells”, basic researchmainly on hematopoietic stem cells in Japan has been strongly recombined with clinical research on leukemia/
hematopoietic diseases to establish an international research center for the pathology of stress hematopoiesis/
leukemia through integrated basic and clinical studies focusing on our theme “stress and senescence/
carcinogenesis.” This program aims to educate young researchers to be more competitive with leading researchers
worldwide.
Research:
Project 1: Interactions between hematopoietic stem cells and their microenvironment (niche)
To elucidate the interaction and changes in the function of hematopoietic stem cells and their microenvironment andniche, and the mechanisms involved in the deterioration and transformation of hematopoietic stem cells in steadystate and stress state. In addition, aging of hematopoietic stem cells will be analyzed and verified from the genomic,
epigenomic and protein levels in an integrated manner. We have been working on mechanism of quality control ofproteins found by Dr. Miharada and his colleagues, which are important for stem cell aging. Through integrated data
analysis and molecular biological and biochemical analyses of individual genes, proteins, and cell functions, Dr.
Suda, Dr. Takizawa and Dr. Umemoto will be able to understand the mechanisms underlying clonal hematopoiesis
in the elderly, who are considered to be premalignant. We will also attempt to develop a control method forhematopoietic stem cells.
Project 2: Aging and malignant transformation of hematopoietic stem cells
To examine the molecular basis for the development and progression of acute myeloid leukemia andmyelodysplastic syndrome caused by abnormal signal, metabolic, and epigenetic regulation specific to cancer
stem cells. Dr. Sashida and Dr. Sheng have been studying the molecular mechanisms of cancer stem cells throughfull advantage of the excellent single cell analysis and data analysis technologies available at Kumamoto University
and its partners in the United Kingdom, Germany, and the United States. In addition, we conduct joint researchwith the NIH, USA that has excellent technology for chromosome structure analysis, where Dr. Kurotaki has visitedmany times since the program launch. We had a symposium on normal an maligrant hematopoiesis in NY in 2023.
In addition, based on the construction of the optical path of thetwo-photon microscope, which Dr. Mizuno developed throughjoint research during his visit to Germany, he carried out intravital
imaging of bone marrow with high resolution, high brightness andwide field, and proceeded with the analysis. Based on these, wewill analyze not only leukemia cells, but also gene expression
abnormalities dependent on interactions with the bone marrowniche, and mechanisms of leukemia development by signal andmetabolic networks.
Project 3: Clonal evolution of leukemia and pathological analysisof adult T cell leukemia
To identify leukemia initiating cells of adult T cell leukemia (ATL)
driven by HTLV1 infection, and to clarify the pathogenesis of ATL
by focusing on epigenetic regulation mechanism. Dr. Matsuoka,
Dr. Yasunaga, and Dr. Sato found that TAX, a viral gene, isintermittently expressed in ATL, and they jointly investigate
the effect of TAX on the dynamics of infected cells and themechanism of escape from host immunity. The results of the joint
research were published in two academic papers. A part of themechanism of disease state progress of leukemia was clarified
from single cell omics analysis of ATL crisis mechanism andfunctional analysis of responsible factor. In addition to ATL, thefindings will be applied to non-ATL leukemias and hematopoietic
neoplasms to examine differences in disease mechanisms.

―8―

The 21st IRCMS Symposium on The Dynamics and Development of Normal and
Malignant Hematopoietic Stem Cells held on Feb. 20-21, 2024

3-3. Diversity and Inclusion
Kids Space:
We take initiative in creating more accommodating working environment for working parents. One of which is theKids Space, where a former PI’s office was repurposed into a room with toys and a playmat for our researchers/staff
with children. The room provides them with secure environment where parents can keep an eye on their children
while they work.
Science Communication - “Gateway to Life Science” :IRCMS has launched the Gateway to Life Science seminar series in 2023. By bringing research and non-researchstaff together more than ever, we aim to make the institute more open to the public. Our institute has always been
based on holding academic events in English, but we have set a new goal to further expand our reach to theresidents who live around our institute and to children, including high school students who are interested in studying
at universities in the future. Through the experience of the pandemic, we have been able to reflect on our trust inscience. In general, research conducted at universities and research institutes is not always accessible to mostpeople. The same can be said for our laboratories, such as basic biology and basic medicine. Through this seminarseries, we will strive to cultivate scientific communication skills among all IRCMS staff. The series is available on our
media outlets such as YouTube.
Kumadai-Hub:
Kumadai-Hub is an organization of “self-proclaimed” young researchers and students at Kumamoto University. It
was founded in December 2019 and has been growing over the last few years. We use the term “self-proclaimed”
because the definition of “young” is ambiguous, so our members gather as “people with a fresh mind”. It started asa free small gathering of researchers/research-enthusiasts from different areas, and has grown in size to about 160
members in a 4-year span.

Group photo at the 3rd Kumadai-Hub Poster Session(2023.12.19.)

We have regular meetings every three months
onsite and online.
―9―Currently, Japanese universities’ policies are full of trendy words such as “young people’s development,”“internationalization,” “joint research,” and “fusion research”. As much as these topics are important, nothingcompares to “our own mindset and actions”. In this sense, we developed our community with the help of researchadministrator, emphasizing the fact that researchers need a fixed space that they come and go when they needand discuss their ideas, similar to what greeks would do in agoras, their public discussion spaces. We have regularquarterly meetings (once every three months) and also hold diverse social events. Recently some collaborations
have emerged through such activities. Despite being always on the look for possibilities of “different fields’
collaboration” and “joint research development” that may emerge in the meetings, this is not our primary goal. We
just want to make Kumamoto University a fun place like a “Salon” that let researchers discuss without barriers. Thisis the Kumadai-Hub’s main mission.

Our cross-disciplinary exchange events areheld in the form of poster presentations. We
discuss the layout of the posters together anddesign them to facilitate the exchange.

Creating opportunities for casual interaction not
only within the university’s conference rooms but
also outside of the university.

Kumadai-Hub is a group open not only to professional researchers, but also to students, technical staffs,
administrative staffs, and all others involved in university research. Despite Kumamoto University having a large
group of researchers spread through its five faculties and three campuses, these researchers don’t actuallyknow each other very well. We had a poster exhibition in 2022 that gave us an opportunity for us to communicate
with each other. The most interesting point was that technical and administrative staffs, who are usually on the
management side, also made and exhibited posters to introduce their own work. Researchers and students tried to
avoid using “cryptic jargons” and focused on having more friendly presentations. The most interesting part was thatthese rules were not something discussed, but people came to this conclusion themselves. Studies and sciences
are not exclusive and should not be restricted to a small group, so we need to be able to share them with everyone
and explain them to anyone. We need more science communicators! This is Kumadai-Hub’s another mission. Lastly,
our community values face-to-face, but we also value online connections. Since we are in Japan, most members
are more comfortable with Japanese, however we also communicate in English and any other language is welcome.
Our greatest value is that we don’t have barriers based on whether you are Japanese or non-Japanese, male orfemale, senior or young, your field of interest, or your job title or position. Kumamoto University has a place where
we can discuss freely, flatly, and happily. Kumadai-Hub have been selected for the Cabinet Office（内閣府）’s Good
Practice in 2024.

Our administrative staff is also creating
and presenting a poster.

One of Kumadai-Hub’s slogans is “Everyone is equal in front of research”.
Administrative staff, technical staff, researchers, and students all support
the community by contributing their own expertise and strengths.
― 10 ―

4. Education
Basic Concepts:
Although IRCMS is not primarily a part of an educational domain of the university, we contribute to life scienceeducation for undergrad and graduate students by delivering lectures in various educational programs, such as
graduate seminars, special lectures, and medical English education. IRCMS also hosts 3rd grade medical students
for a practical course of medical education to let them do research for a couple of months every year.

4-1. Graduate Education
Research Internship:
Our highest priorities are developing global collaborations with renowned institutions around the world and fostering
people exchange with partner institutions. For this end, the IRCMS launched the Research Internship Program in2015 and has given opportunities of hands-on research experiences to undergrad/graduate students who belong
to overseas university/institution. This project supports travel and accomodation cost for students to stay in IRCMS
for a couple of months and carry out a project under the supervision of IRCMS laboratories. Students who havecompleted the internship program will be given an internship certificate by the IRCMS director. In the past, someinterns had good impression on IRCMS during their internship, and later rejoined IRCMS as full graduate students ofKumamoto University, and continued their projects for their PhD thesis.

Fellowship
IRCMS fellowship program was founded in 2016. The aim of this program is to provide financial support toenrolled graduate students with outstanding academic qualifications and strong motivation to conduct cuttingedge biomedical research under the supervision of host IRCMS PIs. The candidate students are selected throughsubmitted paper documents and online or in-person interviews, among those who are enrolled in the Graduate
School of Medical Sciences, Kumamoto University. The selected students are expected to pursue their degrees andgraduate studies in one of IRCMS laboratories.

― 11 ―

D5 Medical and Life Science Seminar in Graduate School Program (D5 Seminar):
IRCMS organizes a yearly seminar course in the curriculum of Graduate School Program of Kumamoto University.
The course is offered to graduate students in English and is composed of monthly seminars given by leading
researchers from Japan and overseas. The seminars are open to all Kumamoto University members (including
PIs, postdoctoral scientists, graduate students, and technical staff members). Young scientists are encouraged
to interact with the speakers during the seminar and discuss their own research with the speakers in face-to-facemeetings before or after the seminar.
74 “D5 seminars” have been arranged by IRCMS since 2017, of which 21 were given by researchers based in Japanand 53 by international scientists [Europe (17), North America (15) and Asia-Pacific regions (21)]. The majority (42/74)
of the D5 seminars were delivered onsite, providing IRCMS members with great opportunities to be updated with
cutting-edge scientific discoveries and to interact with leading scientists around the world.
Themes of D5 seminars vary year by year, centered around research areas covered by IRCMS researches. Past
focuses included stem cell biology, vascular biology, cancer biology and developmental biology. In the upcoming
D5 seminar series (FY2024), we have lined up twelve leading researchers from around the world to talk about
epigenetic regulation in stem cell biology and diseases.

JASSO Agreement Dispatch Program (JASSO 協定派遣 )Taking advantage of our collaborative relationship with KAIST in medical education, we are also formulating
initiatives through the Japan Student Service Organization (JASSO) Agreement Dispatch Program. This program
allows Kumamoto University undergraduate students to experience research in KAIST for one month or more. KAIST
provides accommodation, while JASSO supports the students’ living expenses. Through this program, four medical
students have had valuable experiences under Prof. Injune Kim, Prof. You-Me Kim, Prof. Young Seok Ju, and Won-Il
Jeong. By providing research opportunities overseas with researchers and students of Kumamoto University, IRCMS
is expected to enhance its role as a hub for international collaboration at Kumamoto University. Therefore, we willcontinue to promote initiatives for student and faculty dispatch, including unique activities, in the future.

4-2. Training the Next Generation of Researchers
WIM (Weekly IRCMS Meeting)Weekly IRCMS meeting (WIM) is organized regularly where one or two presenters of not only senior researchers
but also young researchers and students present their research progress every week. WIM has two aims, one
is to improve presentation and communication skills and the other is to seek intra-institutional collaborations: allresearchers basically gather in one conference room and discuss their research progress to help them improve and/or advance their research. Importantly, every IRCMS researcher can have a chance to give an oral presentation in
English and also to be exposed to opinions/critiques by other researchers outside of their own laboratories before
paper submission. Especially for young researchers, this opportunity is quite useful as a pre-training for not only thepresentation in international scientific meetings but also the question-and-answer session. Young researchers canalso see and learn good examples of presentations by senior IRCMS members. Moreover, WIM presentations are
required to be concise and easy-to-understand, and this can contribute to new communications or collaborations,
between not only young researchers but also laboratories. Thus, WIM becomes a kind of core training program foryoung researchers as well as scientific linkage between researchers or laboratories.

― 12 ―

“Science and Me” lecture series:
IRCMS hosts a lecture series called “Science and Me”, consisting mainly of online lectures. We invite sciencerelated professionals to give personal accounts of their respective careers. The aim of this lecture series is to offer
young scientists at IRCMS an opportunity to interact with well-known and well-respected professionals as individual
human beings, each with their unique gifts, limitations and career trajectories. Such interactions serve to strengthen
the self-confidence of young scientists during periods of uncertainty, provide them with role models, and broaden
their perspectives of science-related careers beyond IRCMS.
Since 2021, “Science and Me” lecture series has hosted 20 speakers (12 from Japan and 8 from overseas). All
speakers shared their life stories with generosity and honesty. We had distinguished professors recounting their
dreams when they were budding scientists and mid-career PIs detailing how they coped with scientific research on
one hand, and social and cultural obligations on the other. We had editors from major publishers (Nature, Science
and Cell) reminiscing about why they chose scientific editing over research and how they endeavored to safeguard
publication quality. We also had science writer for a major international newspaper recounting crucial roles playedby scientific research and science outreach during the recent pandemic, and senior executive in the automobile
industry highlighting the importance of strengthening international outlook in maintaining competitiveness of R&D inJapan.
This lecture series had attracted over one thousand participants cumulatively and was enjoyed by both junior andsenior scientists from IRCMS and from other research organizations in Japan.

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5. Research

5-1. Laboratory Introductions

Laboratory of Stem Cell Regulation
Establishment Year

2015

PI			Toshio Suda
Current Members
Yosuke Tanaka, PhD (Lecturer)			
Yabushita Tomohiro, MD, PhD (Postdoctoral Fellow)
			
Ma Wenjuan, PhD (Postdoctoral Fellow)
			
Takako Ideue (Technical staff)
			Miho Kataoka, PhD (Technical staff)
			Yuri Suda (Secretary)

Former Members
Tomomasa Yokomizo, PhD (Lecturer)			
Hiroshi Sakamoto, PhD (Researcher)			Terumasa Umemoto, PhD (Researcher)			Ahad Khalil Nezhad Zein Abad (Postdoctoral Fellow)
			Michihiro Hashimoto, PhD (Postdoctoral Fellow)
			
Yu Matsuzaki (Technical staff)
			
Wei Weng (Technical staff)
			Tamie Endo (Secretary)

Introduction
HSCs give rise to additional stem cells (self-renewal) and to progenitor cells (differentiation). Their fate decision isdetermined both by cell autonomous programs and by the surrounding microenvironment, or niche. My past work
encompasses the study of the intrinsic and extrinsic regulation of HSCs, purification of potent HSCs, analyses ofcell differentiation processes, identification of cytokine signalings in hematopoiesis, and the characterization of HSC
niches. Based on these results, we are currently advancing interventions in niche, particularly in the field of cancer
biology.
Projects
1)Study on HSC self-renewal
Hematopoietic stem cells (HSCs) are multipotent cells, able to differentiate into various types of blood cells inthe body. During embryonic development, it is essential to expand the pool of HSCs for continued growth while
concurrently differentiating into specialized blood cell types. However, until now it has remained uncertain how
these two apparently conflicting processes are achieved simultaneously. To answer this question, we utilized in-vivo
genetic tracing to analyze the formation of HSCs and specialized progenitor cells from intra-arterial hematopoietic
clusters.
In 2018, we found that the hepatic leukemia factor (Hlf) expression marks a developmental continuum between
HSC precursors and HSCs (J Exp Med, 2018). We developed Hlf-tdTomato reporter mouse which revealed that
Hlf is exclusively expressed in aortic hematopoietic clusters and fetal liver HSCs, but not myeloid progenitors andhematopoietic clusters in the yolk sac. HSC specification regulated by the Evi-1/Hlf axis is activated only within Hlfpositive (Hlf+) nascent hematopoietic clusters. These results strongly suggest that HSCs and myeloid progenitors
are generated from distinct and specific HE (hemogenic endothelial cells) (Nature, 2022).
Through kinetic study, the researchers discovered that contrary to established scientific dogma, progenitor cells were
not in fact descendants of HSCs, but HSCs and progenitor cells both develop from the Hlf+ precursor population.
Whether Hlf+ cells would develop into HSCs or progenitor cells was found to be dependent on the expression levelsof the transcription factor Evi1. Hlf+ cells with high levels of Evi1 primarily migrated to intraembryonic arteries andpreferentially gave rise to HSCs. These findings not only shed new light on cell development pathways, but also
open up potentially more efficient avenues of expanding HSC populations for research protocols, which up until now
have required the combined expression of several transcription factors, instead of just one. Their results may also
open up insights onto how cancer cells multiply without differentiating.
To analyze the metabolic change of hematopoietic cells, we have studied the mitochondrial function.

― 14 ―

Evidence of self-replicating hematopoietic stem cells during the embryonic period of hematopoiesis. (left) In
Evi1 heterozygous mutants, the number of HSCs decreases, but the progenitor cells remain unchanged. (right)
Conversely, overexpression of the Evi1 gene in mice leads to an increase in stem cells, and these cells are capableof reconstituting the bone marrow upon transplantation.
2)Mitochondria transfer in hematopoietic cells
We have shown the functionally important intercellular mitochondria transfer between erythroblasts and macrophageniches during anemic stress, hence we are very much interested in finding out how bone marrow stromal cells
support steady state hematopoiesis, as well as mediating stress and aging, particularly from the perspectives ofintercellular mitochondrial transfer (J Exp Med, 2022 ).
However, the exact underlying frequency and triggers of mitochondrial transfer is largely unknown. In our invitro preliminary study of niche and HSC co-culture, niche cells produce prominent cell protrusions containing
mitochondria as well as mitochondria containing extracellular vesicles. We will clarify tunneling nanotubes (TNT),
protrusions and bulbs containing mitochondria in HSCs.
We plan to investigate several stress hematopoiesis models (DNA damage stress, inflammation stress, anemic
stress, aging etc), and transplant BM niche cells from mice with fluorescence labelled mitochondria (mito-dendra2mice) to investigate the presence of mitochondria transport and their functional relevance.
3)Thrombopoietin (Thpo) modulates stem cell quiescence through cell metabolism
On the basis of hypothesis that Thpo regulates HSC numbers and quiescence, we developed and analyzed HSCs
from conventional (Thpo-/-) mice (Haematologica, 2020). In accordance with previous reports, Thpo-/- adult mice
exhibited significantly low number of quiescent BM HSCs. However, when the remaining HSCs in Thpo-/- were
purified, collected and competitively transplanted to lethally irradiated recipient mice, Thpo-deficient HSCs exhibited
similar repopulation potential to wild-type controls. Administration of Thpo-agonist, Romiplostim, to Thpo-/- mice,stimulated the expansion of Thpo-deficient HSCs; the same dose of Romiplostim stimulated proliferation and HSC
exhaustion in wild-type HSCs. RNA-seq analysis of Thpo-deficient HSCs exhibited an enrichment in genes involvedin a variety of cell metabolic processes such as protein synthesis, autophagy and mitochondria metabolism.
Further functional and metabolic analysis of Thpo-/- HSCs revealed that Thpo-/- HSCs exhibit lower mitochondria
volume, mitochondria ATP synthesis compared to wild-type controls. Upon Romiplostim treatment, Thpo-/- HSCs
regained mitochondria volume and ATP production yet at lower accompanying levels of ROS production comparedto Thpo+/+ HSCs. These data suggested that Thpo regulates HSC stem cell quiescence through the modulation of
mitochondrial metabolism. Also, HSCs which are regulated to quiescence in Thpo-/- mice, may be a heterogeneous
subpopulation of HSCs whose reactivity to Thpo signalling differs from most adult HSCs. On the other hand,administration of Romiplostim to mice recovering from myelosuppression exhibited enhanced self-renewal of HSCs.
This indicates that stress activated-HSCs differ in response to Thpo compared to physiological HSCs and supports
the heterogenous nature of Thpo-sensitivity. However, the precise nature of Thpo-/- HSCs (or Thpo independent
HSCs) and how they are regulated without Thpo is yet to be determined (Blood, 2021).
4)A Myb enhancer-guided analysis of basophil and mast cell differentiation
We have analyzed enhancer region of c-myb, which regulates HSC fate. But the results were unexpected. The
transcription factor MYB is a crucial regulator of hematopoietic stem and progenitor cells. However, the nature oflineage-specific enhancer usage of the Myb gene is largely unknown. We identify the Myb-68 enhancer, a regulatory
element which marks basophils and mast cells. Using the Myb-68 enhancer activity, we show a population of
granulocyte-macrophage progenitors with higher potential to differentiate into basophils and mast cells. Single
cell RNA-seq demonstrates the differentiation trajectory is continuous from progenitors to mature basophils in vivo,
characterizes bone marrow cells with a gene signature of mast cells, and identifies LILRB4 as a surface marker ofbasophil maturation. Together, our study leads to a better understanding of how MYB expression is regulated ina lineage-associated manner, and also shows how a combination of lineage-related reporter mice and single-celltranscriptomics can overcome the rarity of target cells and enhance our understanding of gene expression programs
that control cell differentiation in vivo (Nature Comm, 2022).
― 15 ―

Laboratory of Transcriptional Regulation in Leukemogenesis
Establishment Year

2015

PI			Goro Sashida
Current Members
Jie Bai, PhD (Postdoctoral Fellow)
			Mayumi Hirayama, DDS, PhD (Postdoctoral Fellow)
			
Minoru Terashima, PhD (Postdoctoral Fellow)
			
Mihoko Iimori, MT (Technical staff)
			
Ai Hamashima, BS (Technical staff, Master student)			Supannika Sorin, MS (PhD student)			
Eerdunduleng, MD (PhD student)			
Arusyak Ivanyan, MD (PhD student)			
Atsushi Wada, MD (PhD student)			
Keiji Matsufuji, DDS (Technical staff)
			Machiko Mitsushio (Secretary)
Former Members
			
			
			
			
			
			
			
			

Takako Ideue, MD (Researcher)
Gaber Abdallah Abdallah, MD (Visiting Researcher)
Kuan Jew Win, MD, PhD (Researcher (JSPS-RONPAKU))
Wang Yongjie (PhD student)
Takako Yokomizo, PhD (Postdoctoral Fellow)
Yuqi Sun, MD, PhD (PhD student)
Sho Kubota, PhD: (Asistant Professor)
Ratthaphong Phumphu, BS (Visiting PhD student)
Mariko Morii, PhD (Postdoctoral Fellow)

Collaborations		
			
			
			
			
			
			
			
			

Gang Huang (UT Health San Antonio, USA)
Kanlayanee Sawanyawisuth (Khon Kaen University, Thailand)
Kotaro Shide (Miyazaki University)
Mitsuo Oshimura (Tottori University)
Yasuhiro Kazuki (Tottori University)
Nariko Arimura (Tohoku University)
Takatsugu Ishimoto (Japanese Foundation for Cancer Research)
Masatake Araki (Kumamoto University)
Kimi Araki (Kumamoto University)

Introduction
Goro Sashida recieved his MD from Tokyo Medical University in 1996. He then undertook his PhD in medicine under
the supervision of Dr. Kazuma Ohyashiki at the Department of Hematology, Tokyo Medical University in 2002. In
2005, he joined Dr. Stephen Nimer’s laboratory at Memorial Sloan-Kettering Cancer Center (New York, USA; Dr.
Nimer moved to Miami). In 2009, he moved to Dr. Gang Huang’s laboratory at Cincinnati Children’s Hospital Medical
Center (Ohio, USA; Dr. Huang moved to San Antonio), where he became interested in the study of epigenetic
alteration in leukemia. In 2010, he went back to Japan and joined Dr. Atsushi Iwama’s laboratory at Chiba University(Chiba, Japan; Dr. Iwama moved to Tokyo). In December 2014, he established his own research group “Laboratoryof Transcriptional Regulation of Leukemogenesis” at International Research Center for Medical Sciences, Kumamoto
University, and in 2016 he was promoted to Professor. We have been working on molecular mechanisms by whichhematopoietic stem cells develop myeloid malignancies. We are also working on HMGA/HMGN chromatin modifiersin fluke-induced bile duct cancer in collaboration with Dr. Sawanyawisuth in Khon Kaen Univeristy in Thailand and― 16 ―

visiting students.
Projects
To understand how hematopoietic stem cells develop myeloid malignancies:
Myelodysplastic syndrome (MDS) is a group of clonal disorders of hematopoietic stem cells characterized byineffective hematopoiesis, peripheral blood cytopenias, dysplastic blood cells and a predisposition to acute myeloid
leukemia (AML). Given that MDS cells are phenotypically and genetically heterogeneous, the molecular mechanismof the development of MDS is incompletely understood. Recent genome sequencing studies have identified various
somatic mutations of epigenetic modifiers in patients with myeloid malignancies. Aging is known to increase the riskof myeloid malignancies including MDS, conceivably due to environmental stress-induced genetic and/or epigenetic
alterations. Therefore, the accumulation of epigenetic alterations appears to provide pre-MDS stem cells andfacilitate the initiation and propagation of MDS. Our laboratory is working to determine how genetic and epigenetic
alterations collaborate to promote the development of myeloid malignancy by utilizing comprehensive approaches.
Several areas of current investigation on malignant hematopoiesis in the lab include:
1.Understanding how transcription factors and chromatin modifiers drive development of MDS and AML
We have elucidated the epigenomic regulatory mechanisms of RUNX1 transcription factor and MLL1, among others,in the United States and at Chiba University (Zhang Y et al. Blood 2012; Tanaka S, et al Blood 2012). We were thefirst to report the stem cell regulatory mechanism of SF3B1, a splicing factor that is frequently mutated in clonal
hematopoiesis (Wang C, et al. Blood 2014). Furthermore, we established several myelodysplastic syndrome models
using genetically engineered mice with EZH2 and TET2, which are frequently mutated in hematopoietic tumors, toelucidate the pathogenetic basis of the disease due to epigenomic dysregulation (Muto T, et al. J Exp Med 2013;
Sashida G, et al. Nature Commun 2014; Mochizuki-Kashio M, et al. Blood 2015; Hasegawa N, et al. Leukemia 2017).
At Kumamoto University, we are trying to reveal the pathogenetic basis of myeloid malignancies and refractory rareleukemia, focusing on chromatin dynamics and enhancer dysfunction (Sashida G, et al. J Exp Med 2016; Wang C,
et al. J Clin Invest 2018; Kubota S, et al. Nature Commun 2019; Yokomizo-Nakano T, et al. Cancer Res 2020; Bai J,
et al. Oncogene 2021).
2.Understanding how aging and environmental stresses impair normal stem cell and drive development of
myeloid malignancies
We generated inducible leukemia models such as aclinically relevant RUNX1-ETO leukemia murine model(Abdallah MG, et al. Leukemia 2021) and study themolecular mechanisims of age-dependent developmentof leukemia. Recently, we demonstrated that a priorstimulation with bacterial and viral products followed byloss of the Tet2 gene facilitated the development of MDS
via up-regulating target genes of the Elf1 transcription
factor and remodeling the epigenome in hematopoietic
stem cells in a manner that was dependent on Polo-likekinases (Plk) downstream of Tlr3/4-Trif signaling, but did
not increase genomic mutations (See right picture). Our
study proposes the Trif-Plk-Elf1 axis as a potential target
for therapeutic interventions for MDS (Yokomizo-Nakano T,
et al. J Exp Med 2023).
3.Understanding how Trisomy impacts on stem cell function and drives development of myeloid malignancies(e.g. Trisomy 8 and Down syndrome)
We generated murine and human Trisomy models and have been studying the molecular mechanisims of Trisomydependent premature aging in stem cell and development of leukemia.

― 17 ―

Laboratory of Cancer Metabolism
Establishment Year

2015

PI			Masaya Baba
Current Members
Shintaro Funasaki, PhD (Postdoctoral Fellow)			
Hidekazu Nishizawa, MD (PhD student)			
Jinglong Tang, (PhD student)			
Ola Shalaby, MD (PhD student)			
Wu Chang, (PhD student)			
Shoichiro Kuroda, MD (PhD student)
			Ziyang Zhang, (Master student)			
Takumi Shirahama, (Master student)
			Nobuko Irie, (Technical staff)
			Haruka Fukushita, (Secretary)
Former Members
		

		
		
			
Collaborations		
			
			
			

Wenjuan Ma, (PhD student)
Wilan Krisna, (PhD student)
Sally Mehanna, (PhD student/Postdoctoral Fellow)
Eita Uezono, (PhD student)
W. Marston Linehan (National Institutes of Health, USA)
Jonathan R. Keller (National Institute of Health, USA)
Arnim Pause (McGill University, Canada)
Brian M. Iritani (University of Washington, USA)

Introduction
The molecular investigation into Kidney Cancer development began by recruiting patients with hereditary kidneycancers, such as von Hippel Lindau Disease, Hereditary Papillary Renal Carcinoma, and Brit Hogg Dubé Syndrome.
Genetic analysis of these familial kidney cancers unveiled novel tumor suppressor genes and proto-oncogenes,
including VHL, FLCN, and MET. The discovery of these genes not only elucidated the molecular mechanisms
underlying kidney cancer development but also opened up new avenues of research. For instance, the discovery of
the VHL gene contributed to the development of oxygen sensing biology, earning a Nobel Prize.
In 2002, a novel tumor suppressor gene, “FLCN,” was identified as the causative gene for the hereditary kidneycancer syndrome “Brit Hogg Dubé Syndrome.” We found that the FLCN protein interacts with novel proteins FNIP1/2,
which in turn bind to AMPK, a key regulator of energy sensing, and participate in the mTORC1 signaling pathway.
Additionally, we showed that FLCN regulates the activity of the transcription factor “TFE3”. Since 2015, our laboratory
has been dedicated to unraveling the molecular role of the FLCN signaling pathway.

Projects
1.)Molecular function of the FLCN-TFE3 Axis
We generated a conditional Flcn knockout mouse and observed that kidney-specific Flcn knockout mice displayed
hypercellular proliferation and overactivated mTORC1. Our research suggests that Flcn loss induces a metabolic
shift towards oxidative phosphorylation by hyperactivating TFE3, resulting in elevated expression of Ppargc1a.
Additionally, we have shown that the Flcn-Tfe3 axis is crucial for normal hematopoiesis, maintaining bonehomeostasis, phagocyte function, and endothelial cell identity.

― 18 ―

2.)The oncogenic role of TFE3 and its modification for innovative therapeutic approaches.
TFE3 becomes hyperactivated in the absence of FLCN or through the formation of chimeric genes with other genetic
elements. We have generated a mouse model expressing a fusion gene “PRCC-TFE3”, which is found in a sporadictranslocation kidney cancer(tRCC). The PRCC-TFE3 expressing mice demonstrated hyper cell proliferation, resulting
in the kidney cancer development. By utilizing this mouse model, we have identified a novel biomarker, “GPNMB,”
along with RET, as a promising candidate molecule for a groundbreaking targeted therapy. Having established that
PRCC-TFE3 is a constitutively active oncogenic transcription factor, we endeavored to curb its oncogenic activity byimpeding its binding to the target sequences. Furthermore, we conducted a CRISPR/Cas9-mediated genome-wide
screening with PRCC-TFE3 expressing cells to identify a candidate targeting molecule. We have identified CCNC
(Cyclin C), which forms a complex with CDK8/19 and regulates the function of the mediator complex, as a potential
candidate molecule. Indeed, the treatment of PRCC-TFE3 expressing cells with a CDK8/19 inhibitor significantly
suppressed the transcriptional activity of PRCC-TFE3. These findings lay the foundation for the development of novel
therapeutics for kidney cancers with hyperactivated TFE3.

― 19 ―

Laboratory of Stem Cell StressEstablishment Year2015

PI’s Name		

Hitoshi Takizawa

Current Members
Tatsuya Morishima, MD, PhD (Lecturer)			Mayu Kawai, MD. PhD (Postdoctoral Fellow)
			
Pui Yu Ho, PhD (Postdoctoral Fellow)
			
Yuxin Wang, MD (Postdoctoral Fellow)			Alban Johansson, MS (PhD students)			Ismael C. Adolf, MS (PhD students)
			Tomson Kosasih, MS (PhD students)			
Md Naeem Hossain, MS (PhD students)
			
Sayuri Nakata, PhD (Technical staff)
			Yoko Takizawa (Secretary)
			Rina Nagata (Secretary)
Former Members
			
			
			
			
			
			
			

Ryo Sato, (Undergraduate / Assistant technical staff)
Maiko Sezaki PhD (PhD student/Postdoctoral fellow)
Aya Kudo (Undergraduate/Assistant technical staff)
Md. Fakruddin, PhD (Postdoctoral fellow)
Yoshikazu Hayashi, DDS, PhD (Postdoctoral fellow)
Ryo Harai (Undergraduate/Assistant technical staff)
Mio Urata (Technical staff)
Otowa Takahashi (Technical staff)

Collaborations		
Takayuki Yamashita, Fujita Health University
			
Shinji Fukuda, Keio University
			
Paola Betancur, University of California San Francisco, USA			
Roland Huber, A*STAR, Singapore
			Nina Cabezas-Wellscheid, Max Plank Institute of Immunology and Epigenetics, Germany			
Falk Butter, Mainz University, Germany			Pilhan Kim, Korean Advanced Institute of Science and Technology, South Korea
			
Elisa Laurenti, Stem Cell Institute, University of Cambridge, UK			Simon Mendez-Ferrer, Stem Cell Institute, University of Cambridge, UK			Victor Appay, Université Paris-Sorbonne, France			César Nombela-Arrieta, Div. of Experimental Hematology, University Hospital Zurich, Switzerland			Fumio Arai, Div. of Stem Cell Regulation, Kyushyu University, Japan			
Susumu Goyama, Institute of Medical Sciences, The Univeristy of Tokyo, Japan			
Ben MacArthur, University of Southampton, UK			Yosuke Mukoyama, National Institute of Health, USA			
Koji Eto, CiRA, Kyoto University, Japan			
Toshio Suda, National University of Singapore, Singapore			Markus G. Manz, Div. of Hematology, University Hospital Zurich, Switzerland
Visiting Scientists
Momoko Yoshimoto, M.D. Ph.D, Western Michigan University, USA			
Keisuke Horikawa, M.D. Ph.D, Australian National University, Australia			Toshimi Yoshida, Ph.D., Massachusetts, General Hospital, Boston, USA			Taichi Shimura, Kozo Keikaku Engieneering, Inc., Japan			
Koichi Takahashi, M.D. Ph.D, The University of Texas, MD Anderson Cancer Center, USA			
Maiko Sezaki, Ph.D, University of Texas Health San Antonio, USA
			Kanako Wakahashi, M.D. Ph.D, Spanish National Center for Cardiovascular Research, Spain
Introduction and History
Hematopoiesis is one of stem cell-sustained somatic organs in which billions of blood cells are being formed every
day throughout lifetime of an individual. Blood homeostasis is maintained by a very rare population of hematopoietc
stem cells (HSCs) that slowly self-renew and multi-lineage differentiate into functionally mature blood cells via rapidly
dividing lineage committed intermediate progenitors. Such high regenerative capacity of HSC is conserved in a
specialized microenvironment in the bone marrow (BM), often referred to as “BM niche” that supplies pivotal factorsfor HSC maintenance.

― 20 ―

Projects
Our projects to understand physiology and pathophysiology of hematopoietic stem cells are subdivided into thefollowing three specific aims:
1.) Studying human HSC and niche biology by using a novel humanized mouse model with functional humanbone organ
While genetic engineering of animal models have allowed to uncover many vital factors that regulate self-renewal
and differentiation of HSCs, little is known about the cellular and molecular components of the human BM niche.
In this project, we take a developmental tissue engineering
approach that allows to ex vivo generate a human cartilage
template (ossicle) with human adult BM-derived mesenchymal
stromal cells (MSCs), and to in vivo develop functional humanbone organs capable of human HSC quiescence (Scotti,
Piccinini, Takizawa, et. al., PNAS 2013; Fritsch, et al., Exp.
Hematol., 2018). To study human BM niche in steady-stateand during hematopoietic regeneration, we aim to generate
the ossicles derived from normal human BM-MSCs, anddissect cellular and molecular BM niche components by
reengineering the ossicles via genetic manipulation of MSCs.
This transplantable, re-engineerable human bone organ unit willserve as a versatile platform not only to understand human BM
niche homeostasis and disease, but also to preclinically test
newly-developed drugs or develop new therapy for blood cancer.
2.) Innate immune signaling-mediated sensing mechanism in HSC/progenitor
Upon hematopoietic challenges such as infection and inflammation, short-lived immune effector cells are rapidly
consumed at the site of hematopoietic demand, and need to be replenished by HSCs in bone marrow (BM)eventually. Fundamental questions are 1) how the hematopoietic demand in peripheral tissue is sensed andtranslated to hematopoiesis by distal BM-resident HSC, 2) if HSC/progenitor can retain the adaptation programand fine-tune hematopoiesis for future inflammation. In this project, we aim to tackle these questions by using
mouse models in which acute or chronic inflammation is induced upon bacterial infection, microbial infiltration,
autoimmunity and so on. Systemic injection of lipopolysaccharide (LPS, a ligand for Toll-like receptor (TLR)-4),
recapitulating bacterial infection, has demonstrated that LPS directly activates TLR4 in HSCs and decreased theircompetitive fitness through increased division and DNA damage responses (Takizawa et al, Cell Stem Cell 2017).
Bacteria also co-exist with our body and constitute commensal microbe. Upon acute colitis allowing microbial
infiltration, a specific type of microbial species activate BM hematopoiesis through TLRs and direct hematopoietic
progenitors into inflamed lymph node in which they generate anti-inflammatory effector cells to dissolve inflammationand enhance tissue repair (Sezaki et al., EMBO 2022). Given
microbial invasion can occur not only upon gut damage but
also under relatively physiological situations such as ageing,these findings indicate pathogen recognition receptormediated demand-adapted program in HSC, and propose
possibility that chronic inflammation triggered by tissue
damage, ageing, increases risk for development of clonal
hematopoiesis, accumulation of genetic ablations, andmalignant transformation, as observed in the populationbased studies and animal models. We have extended thesefindings to study how inflamed HSC influences lifelong
hemato-immune function.
3.) Understanding of ageing-associated functional changes in HSC and their-derived hemato-immune system
Ageing of hemato-lymphoid system is characterized by thymic involution, expansion of memory B and T cellpool, narrowed spectrum of acquired immune receptors,
favored myelopoiesis at the expense of lymphopoiesis,
clonal hematopoiesis, all of which together lead to increased
susceptibility to infection, and increased risk of myeloid bloodcancer. Some of these ageing phenotypes are attributed
from functional changes of HSCs, including decreased selfrenewal, myeloid biased lineage output, and accumulationof genetic ablations. Here we set out to ask the questions,
1) how relatively intrinsic and extrinsic changes affect HSC
functionality during ageing, e.g., clonal hematopoiesis, and 2)
what is the biological impact of aged HSC-derived alterationsof hemato-immune system on other organ homeostasis,
mainly focusing on HSC competitive fitness and their-derived
inflammatory signals.
― 21 ―

Laboratory of Gastrointestinal Cancer Biology
Establishment Year

2016

PI 			Takatsugu Ishimoto
Current Members
Takashi Semba (Researcher)
			Masaya Yamazaki (Researcher)			Lingfeng Fu (Researcher)			Atsuko Yonemura (Technical staff)			Yoko Yamazaki (Technical staff)
			Akiho Nishimura (PhD student)			
Yiling Tong (PhD student)			
Huaitao Wang (PhD student)			Yuya Okamoto (Undergraduate student)			Emi Hirata (Secretary)			
Tadahito Yasuda (Visiting Researcher)			Tomoyuki Uchihara (Visiting Researcher)			Masahiro Fukuda (Visiting Researcher)
Former Member
Keisuke Miyake (Technical staff)
			Mayu Koiwa (Undergraduate student)			
Kosuke Yamada (Undergraduate students)
			Chiemi Tsutsumi (Secretary)

Introduction
Genetic factors, viral infection, and chronic inflammation are known to be risk factors in the cancer incidence.
These factors not only accumulate direct damage to the cells from which cancer originates, but also dynamicallyalter the surrounding environment. Our previous studies have shown that interactions between cancer cells and thesurrounding tumor microenvironment, such as immune cells and fibroblasts, can lead to cancer progression andresistance to the treatment. By recognizing cancer as a highly diverse tissue, we aim for radical cure of cancer bysimultaneously blocking multiple targets.
Research
1, Elucidation of the mechanism of tumor fibrosis
It is still unclear why scirrhous gastric cancer and pancreatic cancer with severe fibrosis are “intractable” cancers.
We are challenging to elucidate the entire fibrosis signalling network in tumor tissues by combining single-cell
analysis and functional analysis using fibrotic tumor mouse model, focusing on the diversity of tumor stroma. Basedon the comprehensive data, we expect to generate signals that can be applied to tissue fibrosis other than cancer,
and to elucidate the mechanism of carcinogenesis that occurs frequently in fibrotic tissues.
2, Establishment of cancer treatment strategies and biomarkers targeting tumor stroma
Immune checkpoint inhibitors are expected to become a new pillar of cancer therapy, but only a limited numberof patients still benefit from the therapy. Therefore, the establishment of therapeutic strategies that dramaticallyincrease the therapeutic efficacy of immunotherapy is an urgent issue. We have demonstrated that combinedcancer immunotherapy using stromal reprogramming in combination with immune checkpoint inhibitors is effectivein treating tumors that show severe fibrosis. Currently, we are conducting research to identify biomarkers that can beused to select patients who will benefit from this combination therapy.

― 22 ―

3, Research on tissue stem cells and cancer stem cells during carcinogenesis
The origin (roots) of cancer cells vary by organ and tissue type, resulting in a diversity of cancer cells. As a result,treatment options for cancer are complex, and uniform treatment cannot be sufficiently effective. To explore theroots of cancer, we must understand how tissue stem and progenitor cells, which play important roles in tissue
maintenance and regeneration, behave during cancer development. To this end, we are using mouse models that
can trace the differentiation lineage of tissue stem cells to visualize the carcinogenesis process and clarify the earlychanges that occur at the tissue level.
4, Elucidation of the mechanism of cancer progression caused by multi-organ interactions
During the process from cancer development to metastasis formation, cancer cells create a favorable environment
for themselves by interacting with various organs and cells in addition to the primary site of cancer. We aim toelucidate the impact of minute changes that occur through trans-organ communication on cancer progression andto suppress distant metastasis by inhibiting the formation of a favorable environment for cancer.

― 23 ―

Laboratory of Developmental Morphogenesis
Establishment Year

2016

PI			Guojun Sheng
Current Members
			
			
			
			
			
			
			
			
			
			

Wei Weng, PhD (Postdoctoral Fellow)
Rieko Asai, PhD (Postdoctoral Fellow)
Hiroki Nagai, MS (Researcher)Galym Ismagulov, MS (PhD student)
Anil Deka, PhD (Visiting Assistant Professor)
Nagisa Matsubara(Undergraduate Student, Faculty of Science)
Marin Nakakubo (Undergraduate Student, Faculty of Science)
Tomoka Seto (Undergraduate Student, Faculty of Science)
Fumiya Shigaki (Undergraduate Student, Faculty of Science)
Ayaka Yoshida (Undergraduate Student, Faculty of Science)
Koko Ishida (Undergraduate Student, Faculty of Science)

Former Members
			
			
			

Sofiane Hamidi (Postdoctoral fellow)
Amaya Jauregi Miguel (Postdoctoral fellow)
Ruslan Deviatiiarov, PhD (Researcher)
Ratchapon Prakongkaew (Exchange Master Student)

Collaborations		
			
			
			
			
			
			
			
			
			
			
			
			
			
			
			
			

Takaichi Fukuda (Kumamoto University)
Mikako Fujita (Kumamoto University)
Oleg Gusev (Kazan Federal University)
Ruby Huang (National Taiwan University)
Sittipon Intarapat (Mahidol University)
Hiroshi Kagami (Shinshu University)
Hiroshi Kiyonari (RIKEN BDR)
Ruda Lee (Kumamoto University)
Alfonso Martinez-Arias (Universitat Pompeu Fabra)
Tatsuo Shibata (RIKEN BDR)
Woranop Sukparangsi (Burapha University)
Mikiko Tanaka (Tokyo Institute of Technology)
Jean Paul Thiery (Guangzhou Laboratory)
Kazuhiro Wada (Hokkaido University)
Qilong Ying (University of Southern California)
Guojie Zhang (Zhejiang University)
Zhongjun Zhou (University of Hong Kong)

Introduction
Our lab uses the avian animal model and studies cellular and molecular mechanisms regulating cell fate changes
during early embryonic development. Our research focuses on the epithelial-to-mesenchymal transition (EMT)
process during gastrulation, early mesoderm differentiation, extraembryonic tissue development and avian stem cellbiology. We are also interested in understanding the link between developmental and cancer EMTs and that between
normal and abnormal states of mesoderm lineage specification in development and diseases.

― 24 ―

Projects
1.EMT regulation before and during gastrulation
In this project, we study the relationship between cellular morphogenesis (mainly the processes of epithelialmesenchymal transition and mesenchymal-epithelial transition, but we are also interested in other types ofmorphogenesis) and cellular lineage differentiation (Hamidi et al 2020 Development; Sheng 2021 Development;
Sheng et al 2021 Science). In the context of gastrulation, we are currently investigating how EMT transcriptional
regulators can affect the timing of pluripotency exit and embryonic patterning. We are also investigating how thesetwo components of developmental regulation (of shape and function) are coupled (and under certain conditions
decoupled) during embryonic axis formation.
2.Mesoderm lineage specification and extraembryonic mesoderm differentiation
In this project, we study how blood, endothelial and smooth muscle cells are specified after mesoderm formation (thistakes place immediate after gastrulation) (Nagai et al 2018 IJDB; Weng et al 2020 Haematologica) and how thesecells contribute to extra-embryonic tissue function (Nagai et al 2022 Phil Trans RSL). We are currently investigating
how master regulators control the differentiation process of these cell lineages and how the avian embryo canbe adapted to investigate human stem cell differentiation towards these lineages. We are also investigating how
extraembryonic mesoderm cells contribute functionally to the chorio-allantoic membrane (an embryonic organ
involved in breathing).
3.Avian omics and avian stem cell biology technologies
In this project, we aim to develop and optimize avian embryonic stem cells and to use such technologicalbreakthroughs for developmental modeling and for endangered species conservation. We also utilize avian
omics tools to help us gain genome-level understanding of avian development (Deviatiiarov et al 2023 Genome
Biology; Lizio et al 2017 PLOS Biology). Our current project involves using chicken embryonic stem cells to model
gastrulation in vitro, generating embryonic stem cells of peafowls for species conservation, and utilizing single cell
technologies for better understanding of pre-gastrulation lineage heterogeneity, including heterogeneity in primordial
germ cell specification.

A developing chicken embryo with its associatedextraembryonic tissues, the most prominent of which is
the yolk sac. Other extraembryonic tissues include theamnion, allantois and chorion. The latter two fuse to form thechorioallantoic membrane, serving as the embryonic “lung”.
Presence of the amnion defines this group of vertebrates
as the amniotes (birds, reptiles and mammals). Avian
chorioallantoic membrane is functionally and anatomicallyequivalent to mammalian placenta.

― 25 ―

Relationship between embryo proper andextraembryonic tissues in amniotes. Top:avian embryo; bottom: mammalian embryo.
ch: chorion; am: amnion; ys: yolk sac;
al: allantois. Gold color indicates cells of
mesoderm origin.

Laboratory of Multi-dimensional Imaging
Establishment Year

2018

PI			Hidenobu Mizuno
Current Members
Hiromi Mori MS, (Technical staff)
			
Ryoko Koitabashi (Technical staff)
			Elvira Abzhanova MD (PhD student)			
Takamitsu Egashira (Master student)			
Ayami Kohara (Undergraduate student)			Yuzuki Kawae (Undergraduate student)
			Rina Nagata (Secretary)
Former Members
			Madhura Subba Rao PhD (PhD student, Postdoctoral Fellow)
Kazunori Ariyoshi MD (Undergraduate student)

Collaborations		
			
			
			
			
			
			
			
			
			
			
			
			

Hayato Chiba (Professor, Tohoku University)
Takashi Hayakawa (Assistant professor, Nihon University)
Takumi Higaki (Professor, Kumamoto University)
Yusuke Hirabayashi (Associate professor, University of Tokyo)
Takuji Iwasato (Professor, National Institute of Genetics)
Carl C. H. Petersen (Professor, EPFL)
Yoshiaki Tagawa (Professor, Kagoshima University)
Yuji Takihara (Lecturer, Kumamoto University)
Nao Tamagawa (Assistant professor, Kagoshima University)
Masafumi Tsuboi (Assistant professor, University of Tokyo)
Naofumi Uesaka (Professor, Tokyo Medical Dental University)
Tomohisa Toda (Professor, FAU Erlangen)
Keita Tamura (Assistant professor, University of Cambridge)

Introduction
The formation of cerebral neural circuits during development is the basis of higher brain functions such as cognition.
It has been shown that neural activity in the cerebral cortex is involved in circuit development such as axon anddendrite formation (Katz and Shatz, Science, 1996; Mizuno et al., J Neurosci, 2007; Eur. J Neurosci, 2010; Neuron
2014; Rao and Mizuno, Neurosci Res 2021). In recent years, we have discovered using in vivo single-cell two-photon
microscopy that there is synchronous neural activity that is independent of external input (correlated spontaneous
activity) in the developing cerebral cortex (Mizuno et al. al., Cell Rep 2018). Our research goal is to clarify the roleof correlated spontaneous activity in the formation of neural circuits. Also, the Mizuno laboratory is contributing to
the promotion of joint research both domestically and internationally through intravital imaging of various animal andplant tissues (Plant Biotech 2020; Development 2022; Commun Biol 2022; eNeuro 2023).
Projects
Our projects to understand the mechanisms for neuronal circuit development are subdivided into the following two
specific aims:
1.) Elucidating the mechanism of neural circuit formation by correlated spontaneous activity
We have found that correlated spontaneous neural activity exists in the developing cerebral cortex (Mizuno et al.,Cell Rep 2018). However, the causal relationship between neural activity patterns and neural circuit formation is still
unclear.
In this project, we developed a method for simultaneous in-vivo imaging of the activity patterns and cell

― 26 ―

morphology of cerebral cortical neurons (Mizuno et al., J Neurosci 2021). We visualized neural activity patterns
and cell morphology by introducing the calcium indicator GCaMP and the red fluorescent protein RFP. In order tosimultaneously acquire GCaMP and RFP signals, we built a two-photon microscope system that can use excitation
lasers with two different wavelengths. By examining the morphological changes of cells whose activity patterns areobserved, the correlation between neural activity patterns and cell morphological development will be clarified. In
addition, we are developing an in vivo optogenetics system to inhibit normal neural activity patterns. Our strategy
will clarify the causal relationship between neural activity patterns and neural circuit formation.
2.) Elucidating the mechanism of brain neural circuit disorders to improve brain diseases
Cognitive impairment in patients is often caused by brain circuit disorders. Intellectual disability occurs in about
2% of the population, and dementia affects one in four elderly people. For these reasons, there is an extremely highsocietal need for neural circuit reconstruction. However, approaches for circuit reconstruction are limited.
In this project, we will establish a method to induce the reconstruction of neural circuits by applying optogenetic
techniques to the cerebral cortex of disease model animals. As a disease model, we have been using NMDA-typeglutamate receptor-deficient animals and RasGAP NF1 (the causative gene for neurofibromatosis type I)-deficientanimals in which abnormal neural circuit formation have been found in the sensory cortex of the cerebral cortex(Mizuno et al., Neuron 2014; Rao et al., Front Neurosci, 2022). Furthermore, we will develop a less invasive method
for reconstructing neural circuits by stimulating sensory organs. This study leads to the creation of new approach forneural circuit reconstruction.

A. Mizuno Lab members celebrating Dr. Madhura Rao’s PhD degree (March, 2023).
B. Customized intravital imaging system (two-photon microscope) in Mizuno Lab.
C. Research summary. To analyze the dynamics of neural activity in the newborn brain, we conduct intravital imagingof neurons into which calcium indicator is introduced.

― 27 ―

Laboratory of Hematopoietic Stem Cell Engineering
Establishment Year

2019

PI

Terumasa Umemoto

Current Members

Michie Uchikawa (Technical staff)
Alban Johansson (PhD student)
Nazuki Doi (Secretary)

Former Members

Noriko Kataoka (Technical staff)
Shah Adil Ishtiyaq,PhD (Postdoctoral Fellow)

Collaborations

Asumi Yamada (Chiba University)
Kunimasa Ohta (Kyushu University)
Ayako Ishizu (Tokyo Women’s Medical University)







Introduction
Hematopoietic stem cells (HSCs) govern life-long maintenance of blood cell generation through their extraordinary
capacities for “self-renewal” and “multi-differentiation”. Many previous studies suggest that HSCs maintain quiescentstate to suppress excess cell divisions, which prevent them from unexpected differentiation or attenuation of theirstem cell activity, thereby contributing to long-term maintenance of hematopoietic homeostasis. Indeed, HSC
divisions induced by ex vivo culture also promote HSC differentiation or decreased HSC activity. However, uponstress hematopoiesis after irradiation or the administration of anti-cancer drug, HSCs actively divide to regenerate
hematopoietic system through the appropriate balance between self-renewing and differentiation divisions. However,it remains unclear what and how HSCs determine cell fate of their progeny cells during or after cell divisions.
Laboratory of Hematopoietic Stem Cell Engineering was established in 2019 by the support with an unrestrictedgrant from the Chemo-Sero-Therapeutic Research Institute (KAKETUSKEN). Currently, we have studied about themechanism for the regulation of self-renewal division or cell fate in HSCs especially during bone marrow regenerationor during HSC divisions. So far, we have been involved in the publication of more than 15 research papers after
the startup of our laboratory. In particular, we published our research paper, which shows the mechanism of how
HSCs determine cell fate through the combination between mitochondrial metabolism and the change of chromatin
accessibility profiles during
BM regeneration (Umemoto
et al, EMBO J, 2022). This
paper shed light to a novel
relationship between themaintenance of stem-ness,
cell division and mitochondrial
metabolic regulation in
HSCs. When our paper was
published, a review article
that explains our research
was also simultaneously
published (Lisi ‐ Vega et
al. EMBO J, 2022; Figure 1).
We believe that our research
output will contribute to thedevelopment of technologies
for regenerative therapies byusing HSCs.

― 28 ―

Projects
We mainly have two projects that try to understand the mechanism of how to regulate self-renewing divisions or cellfate in HSCs especially during cell divisions from the aspect of metabolic, epigenetic or cytokine signal regulation(Figure 2).
1.) Clarify the mechanism of how self-renewing HSC enable to maintain stem cell activity during cell division.
During hematopoietic regeneration after bone marrow are damaged by irradiation or anti-cancer drug treatment,
many previous studies strongly suggest that HSCs wake up from quiescent state in the steady state initiate cell
divisions including self-renewing division. However, it was difficult to analyze HSCs in this phase since conventional
methods to identify HSCs cannot be applied due to their drastic change of immunophenotype during hematopoietic
regeneration. To solve this issue, we established the way to identify HSCs under almost all situations including
hematopoietic regeneration and enable the accurate and detailed analyses of HSCs during hematopoietic
regeneration (Umemoto et al, EMBO J, 2017; Umemoto et al, J Exp Med, 2018). Taking advantage of this method,
we could identify a unique metabolic state in self-renewing HSCs during bone marrow (BM) regeneration and foundthat this metabolic state is critical for the maintenance of stem cell property during or after HSC division. Currently,
we are trying to understand the mechanism of how this unique metabolism regulates HSC fate during HSC division.
2.) The development of technologies for ex vivo expansion of human HSCs
In this project, we try to apply our findings obtained from mouse HSCs as described above to the development oftechnologies for ex vivo expansion of human HSCs. In particular, ex vivo expansion of BM-derived human HSCs
is not reported yet, while the recent report showed the success of ex vivo expansion of cord blood-derived human
HSCs. The establishmentof ex vivo expansion ofBM-derived human HSCs
is expected to contributeto the development
or improvement ofg e n e t h e r a p ie s usi ng
autologous HSCs.
Indeed, our modification
recapitulating theunique metabolism
observed in selfrenewing mouse HSCs
during hematopoietic
regeneration contributes
to the maintenance ofstem cell phenotypes
during the culture of BMderivd human HSCs.
Currently, we are trying
to establish a technology
to expand human HSCs
by improving our culture
system through additionsof not only further
candidate factors foundin our findings regarding
mouse HSCs but also theengineering approach.

― 29 ―

Laboratory of Skin Regeneration and Aging
Establishment Year
PI

2019

		Aiko Sada

Current Members
Nguyen Thi Kim Nguyen, PhD (Postdoctoral Fellow)
			Tomson Kosasih (PhD students)			Thisakorn Dumrongphuttidecha (PhD student)			
Wenxin Fan (PhD student)			Phung Hung Manh (PhD student)			
Trisha Biswas Shanta (PhD student)
			
Hinata Date (PhD student)			
Ikuto Nishikawa (Master student)			
Takako Keida (Technical staff)
			Rina Nagata (Secretary)
Former Members
			
			
			

Erna Raja, PhD (Postdoctoral Fellow)
Mizuho Ishikawa, PhD (Postdoctoral Fellow)
Yen Xuan Ngo (PhD student)
Bhanubhong Prommalee (PhD student)

Collaborations								Jun Mizuno (National Cheng Kung University, Taiwan)
Chalermchai Pilapong (Chiang Mai University, Thailand)
Edmond Young (The University of Manchester, UK)

Introduction
Tissue stem cells maintain tissue integrity during homeostasis and exhibit remarkable plasticity in response tostresses and injuries. A misregulation of stem cells leads to tissue dysfunction, such as impaired wound healing,
chronic inflammation, tumorigenesis, and aging. Emerging evidence suggests that stem cell populations in adulttissues are heterogeneous and play distinct roles in physiological and pathological conditions.
Our research focuses on elucidating the cellular dynamics and regulatory mechanisms of tissue stem cells during
skin regeneration, inflammation and aging processes. We have identified a novel stem cell population in mouse skinepidermis (Sada et al., Nat Cell Biol 2016) and established genetic tools and molecular markers to analyze thesecells in vivo. Such stem cell proliferative heterogeneity is shown to be present not only in mouse skin, but also in
the other epithelial tissues of the eye and oral mucosa, as well as in human skin. We are now combining cellularand molecular biology methods, mouse genetics, omics analysis, bioengineering, and glycobiology to analyze
the function of biomolecules involved in skin stem cell regulation. Our research goal is to identify the drivers andeffectors of stem cell dysfunction; targeting these factors will allow us to prevent and treat diseases at the stem celllevel, with potential applications in regenerative therapies and future treatments for cancer, aging, and other skindiseases.
Projects
Project 1: Elucidating the Mechanisms of Stem Cell Aging
Aging is the gradual decline of physiological functions over time. The theory of “stem cell aging” proposes thataging is caused by the misregulation or dysfunction of tissue stem cells during aging; however, the crucial drivers
for stem cell aging at cellular and molecular levels still remain largely unknown.
Aging epithelial tissues have impaired wound healing and barrier function and an increased risk of cancer. We
previously found that epidermal stem cell populations with different cell division frequencies coexist in mouse
epidermis (Nat Cell Biol 2016), but the role of these stem cell populations in skin aging was unknown. We recently― 30 ―

reported that a rapidly-dividing epidermal stem cell population declines with age and that maintenance of
extracellular matrix integrity is critical for its regulation (EMBO Rep, 20220). We are currently conducting further
functional analysis of the environmental and stem cell intrinsic factors that induce stem cell aging and skindysfunction.
Project 2: Development and evaluation of bioengineered 3D skin model
The development of in vitro culture of primary keratinocytes and epidermal autografts was the first breakthrough inskin regenerative therapy. Cultured epidermal autografts were successfully transplanted into severe burn patientsin the 1980s. In 2017, genetically modified epidermal stem cells were used to treat junctional epidermolysis bullosa,opening a new avenue for skin regeneration therapy. However, creating a complete skin substitute that mimics
physiological 3D tissue structure and cellular composition in vitro remains a major scientific and clinical challenge.
In particular, it has not yet been possible to reproduce the proliferative heterogeneity of epidermal stem cells that we
found in our mouse experiments under in vitro culture conditions. There is also a lack of markers and tools to assess
the quality of 3D skin equivalents.
Our aim is to develop 3D skin models and evaluate their cellular and molecular components. By optimizing culture
conditions, we aim to reproduce the heterogeneity patterns of epidermal stem cells and their niche environment
in vitro. Future applications of human 3D skin models include regenerative therapy, drug screening, and diseasemodeling.
Project 3: The roles of glycans in skin aging and stem cell function
The presence of glycans determines the structure, stability, and localization of glycoproteins. Glycans play essential
roles in physiological and pathological conditions such as development, tumorigenesis, and inflammation. They arealso required for stem cell regulation by modulating cell-cell and cell-matrix interactions. The study of glycans inmammalian tissue stem cells has been challenging because stem cells are rare and biochemical analysis of glycans
requires large amounts of samples.
Using lectin microarrays, a platform for high-throughput glycan structural analysis, we performed comprehensive
glycan profiling of mouse epidermal stem cells during skin aging (Aging Cell, 2020). We demonstrated thatepidermal stem cells undergo an age-dependent “glycome shift” from high mannose to sialylated complex-typeN-glycans. We identified two lectin probes, a mannose-binder rHeltuba and an a2-3 Sia-binder rGal8N, as novel
biomarkers that preferentially detect young and old epidermal stem cells, respectively. We are currently addressing
how changes in glycans affect protein function and aging phenotype in skin. Glycosylation detected by lectins
serves as a molecular marker of aging, and functional studies offer potential intervention strategies for healthy aging
and longevity.

― 31 ―

Laboratory of Developmental Cardiology
Establishment Year
PI

2021

		Yuichiro Arima

Current Members
			
			
			
			
			
			
			
			

Toshihiro Yamada, MD, PhD (Postdoctoral Fellow)
Xu Yuqing, MS (PhD student)
Akira Fujiyama, MD (PhD student)
Kei Morikawa, MD (PhD student)
Takumi Nagakura, MD (PhD student)
Yasuyo Kimura (Technical staff)
Chiharu Esumi, PhD (Technical staff)
Miho Kataoka (Technical staff)
Megumi Nagahiro (Technical staff)

Former Members

Khlood Ahmed (PhD student)

Collaborations
						

Taro Takizawa (DANDRITE-Nordic-EMBL, Denmark)Suvi Linna-Kuosmanen (University of Eastern Finland, Finland)
Zeeshan Ahmed (Rutgers Robert Wood Johnson Medical School, USA)

Introduction
Arima laboratory has two research themes: the relationship between cardiovascular development and pathology,
and ketone body metabolism. Developmental Origins of Health and Diseases (DOHaD) is a concept that theperinatal environmental stress influences the acquisition of a predisposition of lifestyle-related diseases in adulthood.
Although epidemiological evidences are robust its underlying mechanisms are not well understood. We are trying
to clarify the relation between the cardiovascular development and adult-onset disease through basic research.
In addition, in the process of analyzing the neonatal period, it became clear that ketone body synthesis becomes
active during the neonatal period. To elucidate the significance of ketone body metabolism in the neonatal period,
we have generated knockout mice for HMG-CoA synthase2 (Hmgcs2), the rate-limiting enzyme in ketone bodysynthesis, and discovered a new function of ketone body synthesis. In recent years, it has been suggested that amoderate increase in ketone body concentration may have a positive effect on the body, but we do not know thedetails such as whether the concentration is important or the timing of the increase is important. Therefore, we arealso working on the development of a mouse model that can freely control ketone body metabolism by applying
optogenetical engineering techniques.
In addition, we value morphological evaluation when looking at phenotypes, and are working to develop imaging
methods using micro/nano CT.
Projects
We have two important themes: the relationship between cardiovascular development and diseases, and ketone
body metabolism. In addition, we value seeing the morphology precisely, and we are trying to create a new imaging
technique using the computed tomography (CT).
1. Association between cardiovascular development and adult-onset diseases
Developmental Origins of Health and Diseases (DOHaD) is known as the concept that the perinatal environment
influences disease predisposition trait in adult (susceptible trait to lifestyle-related diseases). In particular, many
reports have been published on low birth weight infants, and epidemiologically, it has been clarified that the risk ofdeveloping hypertension and ischemic heart disease after adulthood increases with low birth weight. However, it
is still unclear why perinatal stress increases the risk of heart disease in the future, and sufficient countermeasures
cannot be taken.
Therefore, in order to verify the DOHaD theory by basic research, our laboratory is conducting analyses to clarify theeffects on the pathogenesis of heart disease by making full use of various mouse models. By advancing this project,― 32 ―

we aim to build a long-lived society where even those who have experienced difficulties in the perinatal period canlive healthy and prosperous lives.

2. Effects of ketone bodies on health and diseases
Ketone bodies are metabolites, which rise during fasting. They are known as an energy source during starvation. In
the process of analyzing the relationship between heart development and pathology in theme 1, We happened to
notice that ketone body synthesis becomes active during the neonatal period. To elucidate the significance of ketone
body metabolism in the neonatal period, we generated knockout mice for HMG-CoA synthase2 (Hmgcs2), the ratelimiting enzyme for ketone body synthesis. Analysis of Hmgcs2 KO neonates revealed that they rapidly develop
fatty liver after birth. It was also found that in insufficient ketone body production, hyper-acetylation of mitochondrial
proteins suppress their function(Arima Nature Metabolism 2021).

Fortunately, We had the opportunity to discover a new function of ketone body metabolism, but at the same time,
various questions and interests arose. Until now, the accumulation of ketone bodies has led to a serious condition
called ketoacidosis, so the negative effects have been emphasized. However, recent reports illuminate thatmoderate increase on ketone bodies has beneficial role for health. But it is very difficult to define this “moderate”
ketone body concentration for health. Therefore, we are also trying to develop a murine model that can artificiallycontrol ketone body metabolism by applying optical engineering techniques.
3. Analysis of micro-architecture using X-ray CT
Computed tomography (CT) is a non-destructive imaging method and an indispensable tool for diagnosing andtreating diseases. The spatial resolution of CT used clinically is about 0.5 mm, but the technological innovation of CT
equipment used in the research field is remarkable, and the highest performance CT equipment is capable of spatial
discrimination of 500 nm. The Faculty of Engineering at Kumamoto University has created an organization called the
X-Earth Center that integrates these latest CT devices, and we participate in this group to establish the collaborative
research between medicine and engineering.
We have developed tissue specific CT imaging methods, such as the structure of murine blood vessels (Arima JAHA
2018, Seya JoVE 2021). Using these methodological advances, we try to develop a new quantitative analysis for
microenvironmental structures.
― 33 ―

Laboratory of Chromatin Organization in Immune Cell Development
Establishment Year

2021

PI

Daisuke Kurotaki

Current Members

Kenta Kikuchi, Ph.D (Postdoctoral Fellow)
Anastasiia Shuliak, MD (Master Student)
Ayu Kurotaki (Technical staff)
Haruka Fukushita (Secretary)

Collaborations

Keiko Ozato (NIH, USA)
Pedro P. Rocha (NIH, USA)
Keji Zhao (NIH, USA)
Leah Rosin (NIH, USA)
Tomohiko Tamura (Yokohama City University)
Koji Yasutomo (Tokushima University)
Utako Yokoyama (Tokyo Medical University)
Kisaburo Nagamune (National Institute of Infectious Diseases)
Yukihiko Hiroshima (Kanagawa Cancer Center)
Kimi Araki (Kumamoto University)
Tokio Tani (Kumamoto University)

Introduction
Our lab started in 2021. The main research focus of my lab is to understand the significance of changes in higherorder chromatin structures and epigenome in immune cell differentiation and responses. We reported on the
dynamics of higher-order chromatin structures in the differentiation and activation of dendritic cells, a type of innate
immune cells, which was published in PNAS (Kurotaki et al. PNAS 2022). We are still analyzing the function of cisregulatory regions in immune cell differentiation and functions. The members at the start of the lab were myself and
Dr. Kenta Kikuchi, a postdoctoral fellow. In 2022, Ms. Ayu Kurotaki joined our lab as a technical staff, and in 2023,
Dr. Anastasiia Shuliak joined our lab as a master’s graduate student. Ms. Haruka Fukushita is also contributing tohelp with administrative work. We are currently collaborating with more than 10 labs in Japan and other countries
using our next-generation sequencing technologies such as Hi-C, CUT&Tag and bioinformatics analysis, and havepublished more than 10 collaborative research papers since the establishment of the laboratory (Yokomizo-Nakano
et al. J Exp Med 2023; Ito et al. J Biol Chem 2023; Umemoto et al. EMBO J 2022; Kanki et al. Cell Rep 2022; andothers).
Project
1.Understanding mechanism underlying establishment and functions of 3D chromatin structure
Higher-order chromatin structuresand epigenetic modifications
dynamically change during celldifferentiation and response toexter nal stimuli; however, theirfunctional significance in theestablishment of cell type-specificand stimulation-dependent gene
expression patterns remains elusive.
Mononuclear phagocytes, suchas monocytes and dendritic cells,
derived from hematopoietic stem
― 34 ―

cells, are essential for various immune responses. In our laboratory, we clarify the functional roles of chromatin
structures, epigenetic modifications, and cis-regulatory elements in the transcriptional control of mononuclear
phagocytes during differentiation and inflammation using next-generation sequencing technologies and genomeedited mice.
Recently, we found that chromatin structures of host defense gene loci are already established in uninfected
dendritic cells before infection and stimulation (Fig, Kurotaki et al. PNAS 2022). We hypothesize that this preparationof chromatin structures is a fundamental principle for rapid immune responses to pathogens. We are currently
testing this hypothesis.
2.Understanding mechanism underlying emergency myelopoiesis
Hematopoietic stem cells (HSCs) generate various types of blood cells through intermediate progenitors. Pioneeringstudies have identified HSCs and multipotent progenitors based on the different patterns of cell surface markers.
Recent advances in technologies such as single-cell RNA-sequencing (scRNA-seq) and in vivo lineage tracing
have led to the realization that HSCs and early progenitors are highly heterogeneous and include subpopulations
with distinct differentiation potentials, suggesting an early lineage specification during hematopoiesis. However, themechanisms underlying the generation of their heterogeneity and early commitment are largely unknown.
To understand the mechanisms of early lineage specification, we performed single-cell RNA-seq in mouse
multipotent progenitor cells. We found a subpopulation of multipotent progenitor cells that express the transcription
factor IRF8, which is an essential transcription factor for dendritic cell (DC) differentiation. The progenitor population
expressing IRF8 preferentially differentiates into DCs in vivo but does not generate significant numbers of
monocytes, neutrophils, or lymphocytes (Fig) (Kurotaki et al Blood 2019). However, the immunological significance ofsuch lineage determination to DCs in early hematopoiesis is unknown. In this project, we use cytokine administration
to mice and several infection models to understand the role of early DC fate determination in host defense to
elucidate the principles of hematopoietic differentiation during infection.

― 35 ―

Laboratory of Proteostasis in Stem Cells
Establishment Year

2021

PI’s Name		

Kenichi Miharada, Ph.D.

Current Members
Kiyoka Saito, Ph.D., Postdoctoral Fellow
			
Maolake Aerken, M.D. Ph.D., Postdoctoral Fellow			Natsumi Miharada, Ph.D., Research Specialist			
Zhu Xinhe, M.S., Research Support Associate			Jingyi Tian, M.D., PhD students
			Machiko Mitsushio, Secretary
Collaborations		
			
			
			
			
			
			
			
			
			
			
			
			

Hiroshi Nittono, Junshin Clinic Bile Acid Institute, Tokyo, Japan.
Roman Zubarev, Karolinska Institute, Solna, Sweden.
Jonas Larsson, Lund University, Sweden.
Martin L Olsson, Lund University, Sweden.
Krzysztof Szade, Jagiellonian University, Poland.
Genta Kakiyama, Virginia Commonwealth University, VA.
Rajgopal Govindarajan, Ohio State University, OH.
Mitsuru Morimoto, RIKEN BDR, Kobe, Japan.
Makoto Makishima, Nihon University, Tokyo, Japan.
Osamu Yoshino, Yamanashi University, Yamanashi, Japan.
Mitsuyoshi Suzuki, Juntendo University, Tokyo, Japan.
Youichi Haga, Toho University, Kanagawa, Japan.
Ryo Kurita, Japanese Red Cross Society, Tokyo, Japan.

Visiting Scientists
			

Youichi Haga, Toho University, Kanagawa, Japan.
Haruki Shirouzu, Waseda University, Tokyo, Japan.

Introduction and History
The process of tissue regeneration and organ development is tightly regulated by complex mechanisms. Stemcells play major roles in these events, which are controlled at multiple levels. Disruption of the system has hugerisks resulting in malignant transformation and developmental failure. Our team has previously reported that proper
control of protein folding is crucial for hematopoietic stem cells (HSC) in order to inhibit the elevation of unfolded
protein response (UPR), and bile acid can support protein folding as a molecular chaperone. Importantly, we havedemonstrated that maternal bile acid is an essential factor for fetal hematopoietic stem cells to expand in the fetalliver. Thus, we are particularly interested in proteostatic and metabolic controls of stem cell regulation and fetalmaternal crosstalk during organ development. Our research output is expected to contribute to future stem celltherapies and the improvement of pregnancy complications.
Kenichi Miharada has started his own research group as a principal investigator (PI) at Lund Stem Cell Center, Lund
University, Sweden, to study the regulation of UPR in HSC. Since 2021, he has become a member of IRCMS as aprofessor and started a new lab; Proteostasis in Stem Cells.

Projects
1.) Discovering molecular mechanisms governing fetal-maternal crosstalk
While the general importance of the maternal body during pregnancy is commonly and broadly understood, themolecular basis for building this complex mechanism has been poorly discovered. The maternal body plays apivotal role in supporting embryonal/fetal development by providing essential nutrients and degrading toxic insults
through the placenta. Since placentas function as a potent barrier that limits the transmission of most proteins,metabolites are considered the main players in the fetal-maternal crosstalk.
In this project, we aim to uncover novel roles of maternal metabolism in fetal development. In particular, we are
studying the importance of maternal bile acid (BA) metabolism during pregnancy. Our previous study reported thatmaternal BAs are transmitted to the fetal liver and support an expansion of HSC by suppressing the induction of
UPR as a chemical chaperone (Sigurdsson et al., Cell Stem Cell, 2016). Recently, we discovered an additional roleof the alternative pathway of BA synthesis, which secures fetal development by degrading toxic oxysterols (Suzuki― 36 ―

et al., submitted). For these studies, we have employed advanced proteomics technologies (TPP, PISA) basedon the thermal stability of proteins. We are now utilizing these technologies to study more fundamental molecularregulations in which metabolites control target proteins both under physiological and pathogenic conditions.

Currently, it has been conceptualized that buds of many diseases are already formed during
embryogenesis, which would affect the whole adult life. Understanding how fetal development is secured
is imperative to reduce such disease risks.
2.) Proteostatic controls and stress responses in HSC
Adult HSCs maintained at a dormant state synthesize proteins at a lower level than other cell types. We and othergroups have discovered that HSC have poor protein folding capacity and are vulnerable to accumulation of un-/misfolded proteins, therefore being predisposed to induction of UPR (Miharada et al., Cell Rep., 2014; Koide et al.,iScience, 2020). However, it remains unclear how HSC keeps protein synthesis low. Furthermore, different protein
folding capacities could mean the existence of proteins having distinct conformational structures in HSCs. We areutilizing TPP and PISA to identify such proteins which are predicted to be key regulators of HSCs.
While the majority of HSCs are in a dormant state, they start to proliferate and give rise to desired cell types undercertain stress situations. Although molecular mechanisms regulating HSC activation under inflammation andinfection have been studied, whether and how HSCs respond to severe anemic conditions is unknown. We havedemonstrated that lipoprotein metabolism is one of key factors controlling the HSC response under acute anemic
conditions (Saito et al., under revision). We are now further studying the extrinsic and intrinsic factors contributing to
the HSC response to anemia.
3.) Establishment of mature red blood cells from immortalized cell sources
Transfusion is an essential treatment for patients who are suffering from anemia and severe bleeding. Since theblood supply for transfusion is insufficient in many countries including Japan, alternative blood sources independentof donation are desired. We have established immortalized human erythroid cell lines (HiDEP, HUDEP, ELLU; Kurita,
Miharada, et al., PLoS ONE, 2013, Soboleva et al., Hum Cell, 2022) and developed efficient differentiation systems(Miharada et al., Nat Biotechnol., 2005; Soboleva et al., Commun. Biol., 2021). We are continuing our challenge toestablish better resources and methods enabling a large scale ex vivo red blood cell production.

Our research focuses on uncovering novel mechanisms triggering diseases through metabolite-protein
interactions, independent of gene deletion/mutation. We have discovered the importance of maternal bile
acid synthetic pathways in fetal development.
― 37 ―

Laboratory of Multicellular Dynamics
Establishment Year2024

PI’s Name

Hiroki Kurihara, MD, PhD

Current Members

Rieko Asai, PhD (WAKAKUSU Researcher)

Visiting Scientists

Sachiko Miyagawa-Tomita, PhD, Yamazaki University of Animal Health Technology, Tokyo, Japan
Akiyasu Iwase, PhD, The University of Tokyo, Tokyo, Japan

Introduction
In our laboratory, we investigate the intricate processes of multicellular dynamics during embryogenesis.
Specifically, we focus on understanding the mechanisms governing early embryogenesis, craniofacial andcardiac morphogenesis, and angiogenesis. Our investigation utilizes a diverse array of techniques including
mouse genetics, experimental embryology of avian embryos, cellular dynamics imaging, single-cell analysis, andmathematical modeling. Additionally, we explore the mechanisms that underlie human diseases based in the context
of developmental biology.

Projects
1. Craniofacial development
The branchial (pharyngeal) arches are a segmental series of bulging structures common and characteristic for allvertebrate embryos. They are mainly formed by migratory cranial neural crests, which give rise to various skeletalcomponents including the jaw and middle ear structures. We have revealed that endothelin-1 (ET-1), first identified
as an endothelium-derived vasoconstrictor peptide, and its receptor ETAR signaling acts as a molecular switch that
determines the lower jaw identity by using mouse genetics.
From this research, several new insights in the field of evolutionary developmental biology have been obtained. In
mouse embryos, the absence of the Endothelin signal results in the failure to form the eardrum and the external
ear canal. In contrast, in chicken embryos, there is instead an occurrence of duplication formation. This reveals
that the eardrum is a product of convergent evolution that independently evolved in mammals and reptiles/birds.
Furthermore, the facial structure of mammals deviates significantly from the common facial development patternfound in jawed vertebrates. It undergoes dynamic evolution, altering the tip of the upper jaw in reptiles andamphibians to create a protruding nose while simultaneously developing new bones to reshape the mouth tip. Thishas been elucidated through experimental embryology and comparative studies, including the analysis of fossils.
Recently, two different recurrent mutations in the Endothelin-A receptor (ETAR) gene EDNRA have been identified
as causative for mandibulofacial dysostosis with alopecia (MFDA), a rare congenital disorder in humans. The
mechanism of its pathogenesis has been elucidated through the use of mouse genetics, pharmacologicalexperiments, and molecular dynamics (MD) simulations. This has revealed that the single amino acid EDNRA
mutations distant from the ligand binding site allosterically affect ligand binding, inhibiting ligand dissociation andincreasing binding affinity.
Mandibulofacial dysostosis
with alopecia results from ETAR
gain-of-function mutations via
a l l o s t e r i c e ff e c t s o n l i g a n dbinding. (from https://www.jci.
org/articles/view/151536)
Endothelin signaling determines the identity of jaw structures.

2. Cardiac development
We have advanced our analysis regarding three cell types that migrate through the cardiac outflow tract andcontribute to heart formation:― 38 ―

1) We clarified that mesodermal cells from theextraembryonic somatopleure, which serves as
the amniotic primordium, migrate into the embryoand differentiate into cardiomyocytes and vascularendothelial cells. We also revealed that BMP and FGF
signals are involved in inducing these differentiations.
2) Through transcriptome analysis of neural crest cells
migrating into the heart and spatial transcriptome
analysis, we unveiled a diverse cell population,
including undifferentiated cell groups resemblingstem cells and precursors, as well as smooth musclelike cells. We also conducted a time-evolving lineage
prediction for these cell populations.
3) In coronary artery formation, we found the
involvement of lymphatic endothelial cells that are
believed to originate from the periaortic capillary plexus around the aortic root during embryonic development. We
demonstrated the role of Semaphorin signals in this process.
Furthermore, through comparative anatomical and developmental analysis of coronary arteries, we elucidated that in
amphibians, teleost fish, and cartilaginous fish, the primary vessels formed during embryonic development directly
supply the heart, whereas, in amniotes (mammals, birds, reptiles), these primary vessels undergo remodeling
during development, forming a new entrance to establish the coronary arteries. This provides a new foundation forunderstanding the causes of congenital coronary artery abnormalities.
3. Angiogenesis
Angiogenesis is a morphogenetic process that produces branching vascular structures during embryogenesis
and various (patho-)physiological conditions. We have identified characteristic cellular behaviors in angiogenic
processes, including dynamic changes in forward-backward movement, tip cell overtaking and resultant cell mixing.
Although the cellular behaviors appear complex and arbitrary, different types of mathematical modeling (stochastic
vs. deterministic) and experimental verification indicated that some deterministic cell-cell interactions are critical for
vascular elongation and possibly branching.
Recently, we found differences in branch-forming capacity among cell types and some regularities in directional
cell movement using in vitro angiogenesis experiments using mouse vascular explants and an endothelial cell line
by refined cell-tracking system. Together with single-cell analyses of cell movement and gene expression, novel
mathematical modeling and experimental verification using constitutional approaches are under way in collaboration
with Professor Tetsuji Tokihiro (Graduate School of Mathematical Sciences, The University of Tokyo, Musashino
University) and his colleagues, to elucidate the possible cellular mechanisms underlying branch formation inangiogenesis.
To elucidate the collective motility characteristics
of endothelial cells that enable sprout elongation,
we analyzed the dynamics of mouse pancreatic
islet microvascular-derived endothelial cell line
MS-1 cells. We discovered that their directional
movement is influenced by cell contacts, with a
combination of linear motion with directionality
and rotational movement as its basis. Throughvarious inhibitors and genetic modifications using
genome editing, it has become evident that theseendothelial cell-specific motility characteristics are
specifically regulated by adhesion molecules suchas VE-cadherin. Currently, we are advancing the
elucidation of the coordination mechanism between
cell motility and transcriptional control by contrasting
gene expression profiles and cell behavioral
phenotypes.

― 39 ―

Laboratory of Epigenetic InheritanceEstablishment Year2024

PI’s Name		

Yuta Takahashi

Current Members
Satoshi Watanabe, PhD (Assistant Professor)
			Rina Nagata, (Secretary)
Collaborations		
			
			
			
			

Jun Wu, UT Southwestern Medical Center
Itaru Imayoshi, Kyoto University
Jae Myoung Suh, KAIST
Keiichiro Suzuki, Osaka University
Juan Carlos Izpisua Belmonte, Altos LabsIntroduction
Epigenetic signatures refer to chemical modifications to DNA or chromatin that play a crucial role in regulating
gene expression without altering the DNA sequence. While it is largely accepted that inheritance of parental traits ismainly driven by the inheritance of genomic DNA, epigenetic inheritance in mammals has been a long-standing andfascinating issue of debate. Unlike genomic DNA sequences, which are relatively stable, epigenetic signatures canpotentially change in response to external stimuli, and the transmission of these changes to offspring suggests thatthe parental generation’s growth environment may influence the phenotypes and of their descendants.
Our laboratory aims to elucidate the mechanisms of transgenerational epigenetic inheritance in mammals using ourunique DNA methylation editing technologies, DNA methylation edited mouse models, and human pluripotent stem
cell models. These endeavors not only serve to increase our understanding of mammalian embryonic development
and evolution but also offer insights into the etiology, diagnosis, and susceptibility of non-genetically inherited humandiseases.

Projects
Exploring the mechanisms underlying transgenerational inheritance of epigenetic signatures
In our previous work, we haveinvestigated the mechanism
by which only CpG islands(CGIs), which are CpG-richregions usually associatedwith promoter regions, remain
unmethylated in the highly
methylated mammalian
genome. In this process, wedemonstrated that insertion of
CpG-free DNA, which doesn’t
contain any CG sequences, into
targeted CGIs induces de novo
methylation of the entire CGI bydisrupting methylation-blocking
machinery in human pluripotent
stem cells (PSCs) (Figure 1) (Y. Takahashi et al., Science, 2017). The acquired methylation is stably maintained evenafter CpG-free DNA removal, extensive passaging, and differentiation. Thus, this approach allows for targeted
CGI methylation editing. Using this approach, we have successfully generated a PSC model with cancer-related
epimutation at MLH1 CGI and corrected an aberrant imprinting in Angelman Syndrome patient specific iPSCs.
Next, we have determined an intriguing question whether transgenerational epigenetic inheritance can take placein mammals using the epigenetically modified mice generated by our unique approach. While transgenerational
epigenetic inheritance is extensively documented in bacteria, protists, fungi, plants, and certain invertebrate― 40 ―

animals, in mammals, it is generally
assumed that bimodal dynamic
epigenetic reprogramming in primordial
germ cells (PGCs) and zygotes serves
t o p re v e n t i n h e r i t a n c e o f a n c e s t r a lepigenetic signatures. However, giventhat it has been reported that epigenome
reprogramming may be incomplete andsome of abnormal DNA methylation at
CGIs may be transmitted from parents
to their offspring in humans, it remains
unclear whether epigenetic signatures
can be passed to offspring in mammals.
We demonstrated that DNA methylationo f p ro m o te r- a s s o c i a te d C G I s c a n
be transmitted from parents to theiroffspring in mice (Figure 2) (Y. Takahashi
et al., Cell, 2023). Using our unique
DNA methylation editing strategies, we
generated DNA methylation edited cells
in which CGIs of two metabolism related
genes, the low-density lipoprotein receptor(Ldlr) and the Ankyrin repeat domain 26(Ankrd26), were specifically methylated,
and their targeted loci were silenced.
Injection of these methylated ESCs into
host mouse 8-cell stage embryos enabled us to generate DNA methylation-edited mice exhibiting altered metabolic
phenotypes. This approach is currently the only way to generate DNA methylation-edited mouse model,
which allows for the exploration of the role of epigenetic regulation in physiological and pathological states in vivo.
Moreover, the acquired methylation of the targeted CGI and the phenotypic traits were transmitted subsequent
generations through both male and female germlines (Figure 2). These observations provide first direct evidenceof transgenerational epigenetic inheritance in mammals, which may not only serve to increase our knowledgeof mammalian embryonic development and evolution, but they may also allow for a better understanding of the
etiology, diagnosis, and offspring’s susceptibility of non-genetically inherited human diseases.
These results regarding the inheritance of methylated CGIs gave rise to a significant question about how the DNA
methylation of CGIs is transmitted to offspring through parental germlines even though there is dynamic epigenetic
reprogramming during development of primordial germ cells and after fertilization. We found that the heritable DNA
methylation of the CGIs is erased in PGCs once and, subsequently, reestablished in next generation embryos after
implantation (Figure 3), indicating that even though sperm and oocyte carry unmethylated CGI to zygote, DNA
methylation can be reestablished in next generation. These results suggest that, instead of DNA methylation,
sperm and oocyte carry “epigenetic memory” to zygotes, and thereby DNA methylation can be reestablisheddepending on the memory. Our laboratory is attempting to investigate the nature of the epigenetic memory
and elucidate themechanisms underlying
transgenerational
epigenetic inheritancein mammals.

― 41 ―

WAKAKUSU Researchers
The Wakakusu Researcher program, established in 2024, is a new initiative designed to support the independenceof young researchers. This project provides institutional support to young researchers in developing their own
research programs, learning how to manage laboratories and to write grants, and interacting with international
researchers, all under close communication with their mentor PIs.
In its inaugural year, the following three individuals have begun their activities as Wakakusu Researchers.

Norika Liu
Macrophages are the most plastic and functionally diverse cells in the blood system and are found in all tissues.
Although macrophages are central to the immune system that regulates tissue homeostasis, they have not received
much attention because of their diversity and lack of integrated understanding. However, macrophages are actuallyan essential cell type in understanding tissue development, physiology, and pathophysiology. Tissue macrophages
are often classified according to their inflammatory status and origin, but recent advances in bioinformatics
technology have revealed that their roles are far more diverse than currently accepted.
Our laboratory focuses on the close relationship between tissue macrophages and blood vessels. Vascular
cells are the major cell population of the heart, perhaps more so than cardiomyocytes. Therefore, the interaction
between macrophages and vascular cells may have a significant impact on cardiac development, physiology, andpathophysiology. Our recent studies have discovered a novel origin for cardiac macrophages, which contributeexclusively to local morphogenesis. In addition, there are several evidences that tissue macrophages play a central
role in vasculogenesis and ultimately organogenesis. We are now investigating the interactive role of macrophages
and vasculatures at the cellular/molecular level using tools to ablate or induce macrophages in a tissue-specificmanner. By combining top-notch bioinformatics and imaging techniques, we are making it possible to unveil
macrophage biology in cardiac development and aging.

― 42 ―

Tatsuya Morishima“Epitranscriptome” includes all the biochemical modifications of the RNA that do not involve a change in theribonucleotide sequence. RNA modification has come to public attention since Novel Prize in Physiology or Medicine
was awarded to the RNA modification work that enabled the development of effective mRNA vaccines against
COVID-19 in 2023.
Transfer RNA (tRNA) is a type of RNA which brings amino acids to rebosomes where proteins are synthesized. It
has been known for decades that tRNA is subject to chemical modifications which stabilize tRNA structure andpromote protein translation through stabilizing decoding process. However, the physiological significance of tRNA
modifications still remains unclear in many organs, including hematopoietic system.
We are working on the role of the mitochondrial tRNA taurine modification enzyme, Mitochondrial tRNA translation
optimization 1 (Mto1), which stabilizes mitochondrial DNA-coded protein translation in hematopoiesis. We foundthat hematopoietic-specific Mto1 conditional knockout (cKO) mice were lethal in the later stage of the fetal life
due to severe anemia.
Mechanistically, mitochondrial
oxidative phosphor ylationc o m p l e x e s w e re s e v e re l y
impaired in the Mto1 cKO fetalliver and this was followed bycytoplasmic iron accumulation.
Overloaded cytoplasmic ironpromoted heme biosynthesis,
which induced an unfolded
p ro t e i n re s p o n s e i n M t o 1
cKO erythroblasts (Morishima
et al. bioRxiv 2023). We are
currently working on the roleof Mto1 in adult hematopoiesis
using inducible knockout
system.

Rieko Asai
My research goal is to understand how our cardiovascular system initiates and is patterned. The heart is the firstorgan to form during embryogenesis, and its circulatory function is essential throughout entire life. While congenital
heart defects affect nearly 1% of births per year, the cell biological mechanisms underlying heart development (socalled ‘cardiac morphogenesis’) are not well understood.
The cardiogenic mesoderm, composed of the first and second heart fields, is the earliest cardiac progenitor
cells and the main source of myocardium. Interestingly, these heart fields bilaterally form along the midline, andthen, the heart tube looping and chamber formation happen with the left-right (LR) asymmetry. To approach
unrevealed mechanisms behind these bilateral-lateral patterning in early cardiac morphogenesis, I focus on thecellular processes, such as cell
migration, proliferation, and polarity.
The centrosome is the microtubuleorganizing center and involved inthese processes. By using highresolution imaging combined withmolecular and genetic approaches
and quantitative/flow dynamics
analyses, I examine the role of thecentrosome behaviors in the cardiac
morphogenesis.
These research interests would impact
the comprehensive understandingof dynamic processes throughdevelopment, maintenance and failure
in complex life systems.
― 43 ―

6. Comments from the Reviewer Board

Yingzi Yang, Ph.D.
Professor
Associate Dean for Research
Harvard School of Dental Medicine
Harvard Stem Cell InstituteJune 14th, 2024
It is a pleasure to read the scientific report of the International Research Center for Medical Sciences(IRCMS) at the Kumamoto University. I recently visited the IRCMS and feel that I am in a good
position to comment on the extraordinary achievements and trajectory of the IRCMS. In short, the
IRCMS at Kumamoto University is at the forefront of biomedical research discovery. In the shorttime since its founding in 2015 in conjunction with the “University Strategy Council”, the IRCMS has
fulfilled all the roles identified for it by achieving tremendous success through the groundbreaking
work of its talented researchers, world-class facilities, and nurturing environment for trainees. By
asking ambitious questions across diverse yet interrelated fields, IRCMS scientists are pushing theboundaries of our understanding of critical areas like stem cell biology, cancer, immunology anddevelopmental biology. Through innovative projects and fruitful collaborations, they are generatinggame-changing insights that will transform patient care. It is tremendously exciting to see what
new frontiers the ambitious and creative labs at the IRCMS will help unlock in the years to come.
Their pioneering spirit of exploration promises many more breakthroughs that advance life sciences
globally.
Cutting-Edge Research and Impact: The IRCMS brings together over 10 dynamic research groups
investigating important questions in hematopoiesis, cancer, immunology, and developmental biology.
Their work is truly pushing boundaries and here are some examples:• In hematopoiesis, they’ve uncovered regulatory mechanisms underlying HSC maintenance,regeneration and ageing.
• Exciting metabolic pathways and epigenetic regulators provide new insights into stem cell
regulation.
• The cancer research is identifying novel drivers with great potential for targeted therapies inrenal cell carcinoma and pancreatic cancer. Understanding tumor microenvironments will betransformative.
• Immunology studies are illuminating key molecular players and transcription factors in dendritic
cell differentiation, an area ripe for application.
• Developmental biology projects on organogenesis are revealing fundamental mechanisms
underlying epithelial-to-mesenchymal transition (EMT) with implications across early embryonicdevelopment and cancer.
Over 300 publications including those in top journals like Cell and Nature clearly demonstrate thecenter’s research excellence and extraordinary impacts at multiple fronts of biomedical sciences.
Collaborations are generating synergies that advance fields like T cell biology and metabolism. The
future is bright as they continue asking ambitious questions!

― 44 ―

World-Class Resources and Partnerships: The cutting-edge core facilities at the IRCMS empower
innovative science. The Flow Cytometry, Bioimaging, and Genomics Cores enable single-cell
analysis, live tissue imaging, and omics approaches critical to projects. A unique strength of IRCMS
is the active and rich international partnerships, which have strengthened the IRCMS throughvisiting researchers
whoResources
contribute and
new Partnerships:
perspectives andFellowship
programs
alsoIRCMS
play
World-Class
Thereagents.
cutting-edgecore facilities
at thea key roleempower
cultivating
the next science.
generation
scientists.
The frequentinternational
symposiums
innovative
TheofFlow
Cytometry,
Bioimaging,and Genomics
Cores andenableimaging, and'omicsapproaches
criticalbenefitted
to projects.
A uniqueseminars single-cellsponsored analysis,by IRCMSlive
aretissueof exceptionallyhighqualityand significantly
researchstrengthof IRCMS isthe active
andandrichtheinternational
exchanges
and collaboration
beyondIRCMS
Kumamotopartnerships,
University. which have strengthened the
IRCMS through visiting researchers who contribute new perspectives and reagents. Fellowship
programs also play a key role cultivating the next generation of scientists. The frequent
Nurturing Talent and Driving Discovery: Graduate and postdoctoral trainees participate in handsinternational symposiums and seminars sponsored by IRCMS are of exceptionally high quality
on internships
and longer-term
fellowships.
100 individuals
from Japan,
Asia,
Europe,and significantly
benefitted
researchOver
exchanges
and collaboration
beyondIRCMS
andandtheNorth America
are beingor have been trained at the IRCMS to date. Exchanges with esteemed
Kumamoto
University.
partner institutions additionally provide global research experiences.
Nurturing Talent and Driving Discovery: Graduate and postdoctoral trainees participate inhands-on internships and longer-term fellowships. Over 100 individuals from Japan, Asia,
Governance: Centrally, the IRCMS is directed by the Director Dr. Hitoshi Takizawa and Vice Director
Europe, and North America are being or have been trained at the IRCMS to date. Exchanges
Dr. Yuichiro
Arima,
with strategic
guidance froman international
Advisory
Board.experiences.
The concept of PI
withesteemed
partner institutions
additionallyprovide global
research(Principal Investigator) has been successfully introduced to realize a departure from the traditionalcourse-based
system inCentrally,
Japan. Thissystem ishasemphasized
the autonomy
of researchers
whoVice
Governance:
the IRCMS
directedby the Director
Dr. Hitoshi
Takizawa and
Director
Yuichiro
Arima,
guidancefrom an international
Advisory
Board. The
independently
planDr.
andconduct
their with
own strategic
studies. To
foster interaction
among diverse
researchers,
conceptofPI(Principal
Investigator)
has
been
successfully
introduced
torealizeadeparture
the laboratories were designed to be open spaces, adopting an open-lab format. Simultaneously,from the traditional course-based system in Japan. This system has emphasized the autonomy
efforts have been made to enhance core facilities, ensuring that experiments could commence fromof researchers who independently plan and conduct their own studies. To foster interaction
the day researchers
arrived.
Core facilities
are jointly governed
to maximize
among diverse
researchers,
the laboratories
were designed
to beefficiency.
open spaces, adopting an
open-lab format. Simultaneously, efforts have been made to enhance core facilities, ensuringthat experiments
could commencefrom theasday
researchers
arrived.
jointly
This organizationalmodel facilitates
IRCMS activities
a unifiedresearch
hub.Core
It hasfacilities
proven arehighlyto maximize
efficiency.
effective governedin establishing
the IRCMS
as a world-leading life sciences research center in under adecade by driving research excellence, cultivating young talent, and leveraging resources throughThis organizational model facilitates IRCMS activities as a unified research hub. It has provencollaborative partnerships. The IRCMS is well-positioned to continue making pioneering biomedical
highly effective in establishing the IRCMS as a world-leading life sciences research center indiscoveries
on aninternational
scale.researchWithin a excellence,
decade, thecultivating
IRCMS hasestablisheditselfleveraging
as a leader
under
a decadeby driving
young
talent, andby fostering
excellence,
empoweringcollaborative
discovery,
andnurturingyoung
scientists.
With
resources through collaborative partnerships. The IRCMS is well-positioned to continue
makingpioneering
discoveries
onpromises
an international
scale.
Within a decade,theadvance
IRCMS has
their ambitious
spirit biomedicalof exploration,
the futureeven more
breakthroughsthat will
establisheditselfTheas aidealeaderby fostering
excellence,
discovery,life sciences
worldwide.
of fusion
environment
and empoweringscience that collaborative
IRCMS will launch
as andnurturingyoung
scientists.
With
their
ambitious
spiritofexploration,
thefuturepromiseseven
an initiative “communication-based science” will be transformation in promoting multi-disciplinary
more breakthroughs that will advance life sciences worldwide. The idea of fusion environment
collaborations.
The IRCMS’s
continued
success
be exciting
to witness! These said,
I hopewill
thatand sciencethat IRCMS
will launch
as willan initiative“communication-based
science”
bethe university
considerstreating
thisten-yearperiodasaformofTenure
Track
for
PIs,
allowing
transformation in promoting multi-disciplinary collaborations. The IRCMS's continued successdistinguishedindividuals
to witness!
transitionThese
into permanent
positions,maintainthe crucial
productivity
will beexciting tosaid, I hopethat thethus
university
considerstreating
this ten-year
periodas a form of Tenure Track for PIs, allowing distinguished individuals to transition intoand stabilityof IRCMS.
permanent positions, thus maintain the crucial productivity and stability of IRCMS.
Sincerely yours,
Sincerely yours,

YingziYang
Yingzi
Yang
Professorof Developmental
Biology
Professor of Developmental
Biology
Harvard School of Dental Medicine
Harvard School of Dental Medicine― 45 ―

Akihiko YOSHIMURA
Professor
Tokyo University of Science
Research Institute for Biomedical Sciences

June 16th, 2024

General Comments
I have read the Scientific Report of the International Research Center for Medical Sciences (IRCMS)
at Kumamoto University and am very impressed. Having had the opportunity to visit IRCMS several
times and engage with PIs and students, I feel well-positioned to comment on the achievements andchallenges of IRCMS. Under the leadership of former Director Toshio Suda, IRCMS has introduced
a new research system characterized by transparent and effective governance and an international
research environment. This innovative approach has set IRCMS apart from other research centers
in Japan. The efforts made over the past ten years are commendable. One unique strength of
IRCMS is its active and rich international partnerships, with visiting scholars enhancing its research.
Due to former Director Suda’s focus on hematopoietic research, IRCMS has attracted many bloodrelated researchers, including those specializing in blood stem cells, the immune system, and thevascular system. This focus makes it easier to see the direction of the project and positions IRCMS
as a leading center for hematopoietic research worldwide. The center is also gaining internationalrecognition through various joint research projects and exchange programs with overseas
institutions. With many graduate students coming from abroad, I am confident that IRCMS willcontinue to develop as a globally recognized institute.
Scientific Achievements
Over the past nine years, IRCMS has published 306 original papers, with about 20% in top journals
such as Cell and Nature, showcasing its strength. The international collaboration is evident, with 61%of the papers coauthored internationally, enhancing IRCMS’s global presence. Many of these papers
are considered cutting-edge and significant globally. Additionally, the number and total amount ofgrants received have tripled since IRCMS’s establishment.
Human Resource Development
The fellowship programs at IRCMS have been playing crucial roles in fostering the next generation ofscientists. Over 100 individuals from Japan, Asia, Europe, and North America have received training
at IRCMS, significantly contributing to research exchange and collaboration. Graduate students andpost-doctoral trainees benefit from practical internships, long-term fellowships, and global researchexperiences through exchanges with esteemed partner institutions.
Governance
Currently, IRCMS is governed by Director Hitoshi Takizawa and Deputy Director Yuichiro Arima,with strategic guidance from an international advisory board, which is commendable. IRCMS has
adopted a new personnel system, including stipends and cross-appointments, setting it apartfrom other Japanese universities. This system has proven effective in establishing IRCMS as aworld-leading life science research center in less than a decade by identifying young talent PI
and supporting their research excellence, and leveraging collaborative resources. IRCMS is wellpositioned to continue pioneering biomedical discoveries on an international scale.

― 46 ―

Research Institute for Biomedical Science
Tokyo University of Science2669 Yamazaki, Noda-shi, Chiba, 278-0022, Japanand leveraging collaborative resources. IRCMS is well-positioned to continue pioneering biomedical
discoveries on an international scale.
Future Challenges
FutureChallenges
To support
IRCMS’scontinued development, I offer a few suggestions:(1) Research
IRCMSdevelopment,
has promoted
To supportDirection:
IRCMS's continued
I offerinterdisciplinary
a few suggestions: research addressing criticalbiologicalproblems
andhumanWith former research
Directoraddressing
Suda’s criticalguidance,the(1) Research
Direction:
IRCMS
has diseases.
promoted interdisciplinary
biologicalhematopoietic
has seen
considerable
success.
forward,theIRCMS
looks incorporatingproblems field
and humandiseases.
With former
Director Moving
Suda's guidance,
hematopoietic
field has seen
oncology,
developmental
biology,regeneration,and immunology,
aimingto becomeaconsiderable
success. Moving
forward,IRCMS looks incorporating
oncology,
developmental
biology,broadregeneration,
research center.
In
this
case,
it
is
crucialtopresentacohesive
research
philosophy
and immunology, aiming to become a broad research center. In this case, it is crucial toencompassing
allfields.research
Additionally,
the differences
betweenthelong-termandpresent a cohesive
philosophy
encompassinginallsignificancefields. Additionally,
differencesinshort-term
researchlaboratories
in
IRCMS
needs
clarification.
It
may
be
importanttoconsidersignificance between long-term and short-term research laboratories in IRCMS needs clarification. It
the difference
from thetoInstituteEmbryology
andforGenetics
Kumamotoand
University.
may be importantconsiderfor
theMoleculardifference fromthe InstituteMolecularatEmbryology
Genetics
Alternatively,
possible
integration
of
the
two
stronginstitutes
may
be
worth
discussing.
These
at Kumamoto University. Alternatively, possibly integration of the two strong institutes
maydecisionsshouldinvolve
input
from
the
International
Advisory
Committee
anddomestic
experts,
worthwhile to be discussed. These decisions should involve input from the International Advisory
but ultimately, the leadership of the Director will be pivotal.
Committee and domestic experts, but ultimately, the leadership of the Director will be pivotal.

(2) Maintenance as the University-linked Institute: Originally established with a 10-year time limit,(2) Maintenance as the University-linked Institute: Originally established with a 10-year time limit,
IRCMS has achieved significant success. It is natural to consider extending its development for
IRCMS has achieved significant success. It is natural to consider extending its development for another
another 10 or 20 years. The issue of the institute’s time limit needs to be discussed, potentially
10 or 20 years. The issue of the institute's time limit needs to be discussed, potentially considering
considering changing the institute’s name or merging it with another affiliated institute.
changingshould
the institute's
nameinoramerging
with anotheraffiliatedinstitute. Continuation should be
Continuation
be planned
way thatit ensures
futuredevelopment.
planned in a way that ensures future development.
(3) Issues Related to the Status of Researchers: IRCMS has a 10-year limit for hiring researchers,(3)theIssuesRelated ofto theStatus of Researchers:
IRCMS
has a 10-year
limit for hiring researchers,
with
with
possibility
transitioning
to a permanent
positionafter reappointment
review. Earlythepossibilityoftransitioning
toapermanent
position
after
reappointment
review.
Early
PIs
have
begun
PIs have begun this review process, and their transition to permanent status is expected tothis review
process,theandprecarious
their transition
to permanent
status istenure
expected
be smooth.
However, thebe smooth.
However,positionof a 10-year
fortoyoung
PI investigators
precarious
positionofa10-yeartenurefor
young
PI
investigators
needs
consideration.
The
possibility
needs consideration. The possibility that young talented researchers may choose morestablethat young
talented or
researchersmaychoosebe
morestable universities,
institutes,often
or industries
cannot beuniversities,
institutes,
industries
cannotignored.
Other universities
link evaluation
ignored. Other
universities often
link evaluationand promotion,
conducting
evaluations
and promotion,
conducting
evaluations
relatively
early (about
5 years)
and relatively
decidingearlyon(aboutcontinued
5 years) andemployment,
deciding on expiration,
continued
employment,or shiftingKumamoto
to lifetime employment.
expiration,or shifting
to lifetime
employment.
University
shouldKumamoto
considerUniversity
this approachto maintainproductivity
and stability
at andIRCMS
by atallowing
should consider
this approachto maintainproductivity
stability
IRCMS
excellent
personnel
to transition
to permanent
positions
aspositions
soon asas
possible.
by allowing
excellent
personnel
to transition topermanent
soon as possible.
(4) Financial
Issues:
While
IRCMS
is a
withaasignificant
significant
presencein Japan,(4) Financial
Issues:While
IRCMS
is uniquea unique institution
institution withpresencein Japan,furtherfurthermeasures
measuresareneededtoenhancethispresence,
particularlyinsecuring
stableoperatingare needed to enhance this presence, particularly in securing stable operating funds.
funds.Kumamoto
KumamotoUniversity
University
shoulddevelopstrategies
to theensurethe long-term
financialshoulddevelopstrategies
to ensurelong-term
financial stability
andstability
growthand growthof IRCMS.
Challenge
to PremierWorld PremierInternational
Center(WPI)
Initiative(WPI)of IRCMS.
Challenge
to World
International
Research ResearchCenter Initiative
programisprogramisimportant.
In
this
case,
IRCMS
shouldhave
aninitiative
among
variousfacultiesofimportant. In this case, IRCMS should have an initiative among various faculties of Kumamoto
Kumamoto
University.
University.
Sincerely yours,
Sincerely yours,

Akihiko YOSHIMURA
Akihiko
YOSHIMURA
Professor
Professor
Tokyo University of Science
Institutefor Biomedical Sciences
TokyoResearch
Universityof Science
Research Institute for Biomedical Sciences― 47 ―

May 17th, 2024
May 17th, 2024
To whom
it may concern,
I wasvery impressedby the remarkable progress of IRCMS over just nine years. The commitmentof all its members has undoubtedly driven this outstanding performance, particularly under the
I was very of
impressedby theremarkableprogressToshio
of IRCMS
overandjust nine
years.director
The commitmentof allits
leadershipfounding
director
Professor
Suda
current
Professor
HitoshimembersI has
undoubtedly
drivenperformance,
particularly
under the leadershipof continuous
founding
Takizawa.
wouldlike to offer
a thisfewoutstanding
suggestions
from an external
perspective
to aid the
director
Professor
ToshioSuda
andcurrent
director
Professor
HitoshiTakizawa.
I
would
like
to
offer
a few
progress of IRCMS in Kumamoto.
suggestions from an external perspective to aid the continuous progress of IRCMS in Kumamoto.

IRCMS has pursued barrier-free research, embracing global collaboration. The center has hosted
IRCMS haspursuedandbarrier-freeresearch,
globalcollaboration.
The centerhas hosted
numerous
scholars
students
from embracing
around theworld
for symposia,
seminars,andnumerous
exchangescholarsandstudents
fromaround
the
world
for
symposia,
seminars,
andexchange
programs
annually
since
programs annually since its inception. It has also dispatched students to other countries, including
its inception.
It has also
dispatchedstudents
to otherincludingSouth partner
Korea. KAIST
based inSouth
Korea. KAIST
based
in SouthKorea
has countries,
been a key
overseasof IRCMS,
withSouth Koreafrom
has been
overseas partnerof IRCMS,
with researchers
frominstitutions
visiting
each
researchers
botha key
institutions
visiting
each other’s
campuses
forbothjoint
symposia,
seminars,other's
campusesfor
joint
symposia,
seminars,andcollaborations.
Additionally,
IRCMS
has
supportedand collaborations. Additionally, IRCMS has supported multidisciplinary research tackling ultimate
multidisciplinary
researchtacklingdiseases.
ultimate biologicalquestionsand humanachievements
diseases. To stimulate
biologicalquestionsand human
To stimulate
collaboration,
should becollaboration,achievements
shouldbeevaluatedwithoutbias;
forexample,co-first
authors
and co- be
evaluated without bias; for example, co-first authors and cocorresponding authors should
corresponding
should beregardlessconsidered ofequal
contribution
their position in theconsideredequalauthors
in contribution
theirinpositionin the regardlessauthorshipoflist.
authorship list.
IRCMS operates core facilities equipped with high-end devices, with staff members providing stateof-theart
Its central
serverwith
is particularly
timelywith
in thisof datascience.
Importantly,
IRCMStechnologies.
operates core facilities
equipped
high-end devices,
stafferamembers
providingstate-of-thesustaining
thisservicerequiresstablefunding
to
keep
pace
with
rapid
technologicaladvances.
art technologies. Its central server is particularly timely in this era of data science. Importantly, sustaining
this service requires stable funding to keep pace with rapid technological advances.
IRCMS maintains a sound balance between education and research. The center offers a variety ofeducation
programsa soundand hassuccessfully
recruitedandmany
graduatestudents
abroad.
Ensuring
IRCMS maintains
balancebetween education
research.
The center
offersfrom
a varietyof education
thatprograms
graduatecomplete
their theses
without students
dropping
outabroad.
is crucial.
Manyresearchers,
andstudents
has successfully
recruited
many graduate
from
Ensuring
that
graduate
includingpostdoctoral
fellows,
have
workedat
IRCMS.
While
theseexperienced
scholars
students complete their theses without dropping out is crucial. Many researchers, including postdoctoralare
expected
produce
outputs,
they stillneed sophisticatedto becomefellows, tohave
workedsignificant
at IRCMS.research
While theseexperienced
scholars
are expected tomentoringproduce significant
matureinvestigators.
The
centercan
supporttheir
careerdevelopment
throughvariousmeans
research outputs, they still need sophisticated mentoring to become mature investigators. The center suitedcanto IRCMS.
support their career development through various means suited to IRCMS.
TheThestatustransitionis quitedynamic,
with approximatelyone
newandPI anotherjoiningPIandstatusofof laboratorylaboratory transitionis quitedynamic,
with approximately
one new PI
joining
anotherPI
leaving
each
year.
While
this
dynamic
natureallowsfororganizational
flexibility,
leaving each year. While this dynamic nature allows for organizational flexibility, lab stability is critical forlab
stabilityis criticalfor sustained
Theremembers
are alsoinmany
past
various job
sustained
productivity.
There areproductivity.
also many pastvariousjobmembers
positions, inparticularly
positions,particularly
administrative
staff,
secretaries,
andtechnicians.
Offering
promotion
administrative staff, secretaries, and technicians. Offering promotion opportunities or permanent
job
opportunities
or permanent job positions can help retain and recruit talented investigators. Extending
positions can help retain and recruit talented investigators. Extending job periods can also enhance job
job periods can also enhance job stability.
stability.
I eagerly anticipate that IRCMS will thrive as a global hub institute, building on its past and current
I eagerly anticipate that IRCMS will thrive as a global hub institute, building on its past and current
commitment to research and education.
commitment to research and education.

Injune Kim, PhD
Injune Kim, PhD
Professor
Professor
Korea Advanced Institute of Science and Technology
Korea Advanced Institute of Science and Technology― 48 ―

7. Discussion and Future Prospects

Discussion:
The IRCMS (International Research Center for Medical Science) established within Kumamoto
University in 2015, has successfully developed its research under the main themes of Stem Cell andCancer, achieving numerous accomplishments. The concept of PI (Principal Investigator) has been
introduced as an attempt to realize a departure from the traditional course-based system in Japan. In
other words, it has emphasized the autonomy of researchers who independently plan and conduct
their own studies. To foster interaction among diverse researchers, the laboratories were designed to
be open spaces, adopting an open-lab format. Simultaneously, efforts have been made to enhancecore facilities, ensuring that experiments could commence from the day researchers arrived.
Additionally, by adopting English as the official language and actively recruiting foreign PIs, thecenter has played a significant role as an international research hub. The recruitment of faculty,
postdoctoral fellows, and interns through international open calls has been progressing smoothly.
Operating independently without affiliation to any faculty or institute, the research center functions as
a flexible organizational entity alongside IROAST (International Research organization of Advanced
Science and Technology), providing a certain degree of academic freedom and agility to ensure
innovation. However, this flexibility also comes with a downside, as it may lack stability.
Currently, the application of the 10-year rule for non-regular employment is posing a challenge.
Personally, I hope that the university considers treating this ten-year period as a form of Tenure Track,
allowing distinguished individuals to transition into permanent positions, thus addressing the stability
concerns.
At the same time, it is imperative that this institute attains a more esteemed standing, positioning
itself favorably to secure grants for cutting-edge advancements in life sciences, particularly within
the realms of stem cell research and cancer studies. I am confident that IRCMS will emerge asa pivotal player in the global communication of life sciences over the next decade. This will beachieved by fostering robust collaborations on an international scale.

Toshio Suda

― 49 ―

Future Prospects:
While a number of achievements such as establishment of new organization and research
environment, development of global collaboration, and creation of human knowledge in science,
all of which are primary goals of IRCMS, has been made for the past, we realize that there is anincreasing number of things to be improved for coming years. This is because as science grows,
system and people must grow together. I would envision the next step for us to move forward isdeveloping a modern research system that has a certain degree of flexibility and sustainability toadapt our research activities to scientific and social demands changing day by day and year by
year.
First, I think a fusion between research and administration is absolutely necessary to move things
forward efficiently and effectively. It has been long standing mindset in traditional Japanese
university system that these two elements are separated from each other and work unidirectionally,
from research to administration, often causing an unnecessary doubt and conflict between them.
Researchers think administrators should serve them, while administrators think researchers can
ask them for anything they need (on the one hand, bureaucratic, on the other hand, authoritarian).
However, throughout entire IRCMS operations, we have learned that mutual trust and close
communication between researchers and administrators is a key to formulating the next generation
university system and advancing research for the future.
Second, new discoveries often come with the development of new technologies. One of the recent
trends that drives cutting-edge science is multi-disciplinary research. I think developmental andstem cell biology have relatively good compatibility with emerging technologies in terms of theirapplication to biology and medicine. I personally believe that life science will develop more if it isfused with other disciplines, such as physics, chemistry, to create new knowledge and technologies,
as exemplified by mechanobiology, redox biology, synthetic biology, space biology and many others.
Such fusion science will help us to better understand physiology and pathophysiology, and beyond
that, open a new door for future biomedicine.
To brew the idea of fusion environment and science, IRCMS will launch an initiative, called“communication-based science” with a flat organization, where everyone feels free to discuss
together and cooperate hand in hand. I hope that this initiative will make IRCMS identity more visible
and unique as a research hub to be recognized by and connected with the world, and to reward our
dedication to advance science and increase knowledge for the future. I am excited to see what will
happen in the coming years.

Hitoshi Takizawa― 50 ―

8. Appendix

8-1. List of Staff and Students

IRCMS Member as 2024.2.1.
Administrative Staff:
Tetsuji Kiyonaga (Division Manager)
Yuki Yoshioka (Chief)
Mai Matsushita
Nanako Watanabe
Ryunosuke Naito
Secretary:
Haruka Fukushita
Machiko Mitsushio
Nazuki Doi
Rina Nagata
Yoko Takizawa
Core Facility Staff:
Akifumi Kiyota, PhD
Hanami Sakai,MA
Natsumi Miharada, PhD
Technician:Ai Hamashima
Ayu Kurotaki
Chiharu Esumi, PhD
Hiromi Mori, MS
Megumi Nagahiro
Michie Uchikawa
Miho Kataoka, PhD
Mihoko Iimori, MT
Natsumi Miharada, PhD
Nobuko Irie
Ryoko Koitabashi
Sayuri Nakata, PhD
Takako Ideue
Takako Keida
Xinhe Zhu, MS
Yasuyo Kimura
Yuxin Wang, MD
Researcher:
Hitoshi Takizawa, PhD (PI) (Director)
Yuichiro Arima, MD, PhD (PI) (Vice Director)
Aiko Sada, PhD (PI)Daisuke Kurotaki, PhD (PI)
Goro Sashida, MD, PhD (PI)
Guojun Sheng, PhD (PI)
Hidenobu Mizuno, PhD(PI)
Kenichi Miharada, PhD (PI)Masaya Baba, Md, PhD (PI)
Terumasa Umemoto, PhD(PI)Toshio Suda, MD, PhD (PI) (Ex. Director)
Hiroki Nagai, PhD
Jie Bai, PhD

Kenta Kikuchi, PhD
Kiyoka Saito, PhD
Mariko Morii, PhD
Mayu Kawai, MD, PhD
Mayumi Hirayama, DDS, PhD
Motomi Osato, MD, PhD
Pui Yu Ho, PhD
Rieko Asai, PhD
Shintaro Funasaki, PhD
Tatsuya Morishima, MD, PhD
Tomohiro Yabushita, MD, PhD
Wei Weng, PhD
Yosuke Tanaka, PhD
Student:
Ai Hamashima
Akiho Nishimura, MD
Akira Fujiyama, MD
Alban Johansson, MS
Anastasiia Shuliak,MD
Arusyak Ivanyan, MD
Eerdunduleng,MDElvira Abzhanova, MD
Galym Ismagulov, MS
Hidekazu Nishizawa, MD
Hinata Date, MS
Ikuto Nishikawa
Ismael C. Adolf, MS
Jinglong Tian, MD
Kei Morikawa, MD
Md Naeem Hossain, MS
Ola Shalaby,MD
Phung Hung Manh, MS
Shoichiro Kuroda, MD
Supannika Sorin, MS
Takamitsu Egashira
Takumi Nagakura, MD
Thisakorn Dumrongphuttidecha, MS
Tomson Kosasih, MS
Trisha Biswas Shanta,
Wenxin Fan, MS
Wu Chang, MD
Yuqing Xu, MS
Ziyang Zhang

Previous Members* List name only
Administrative Staff:
Anett Iwamoto
Hitomi Shimizu
Izumi Mori
Kunio Otaguro
Masae Sakata
Miho Shinmoto
Minako Nishikawa
Naoto Hamada
Noritaka Matsuki
Ritsuko Anzai
Seika Onishi
Setsuyo Nishida
Shinya Murakami
Shuhei Yagi
Takaaki Jo
Takeshi Nishikawa
Tomoe Goto
Yousuke Kondo
Yuji Uchiyama
Secretary:
Ayako Ishida
Chiemi Tsutsumi
Emi Hirata
Junko Kurowarabi
Junko Uemura
Kaori Tomoda
Miho Miike
Minako Yoshino
Nao Syogenji
Noriko Tokushige
Tamie Endo
Yui Sakuma
Yuka Endo
Yukari Ando
Yuri Suda
Core Facility Staff:
Hisanori Wakamatsu
Hitomi Nagayama
Keisuke Miyake
Ryoko Koitabashi
Subinoy Biswas
Technician:Atsuko Yonemura
Aya Kudo
Kanako Nasu
Keisuke Miyake― 51 ―

Mariko Inoue
Michiyo Tokunaga
Mio Urata
Natsuki Morinaga
Noriko Kataoka
Otowa Takahashi
Ryo Harai
Ryo Sato
Takako Ideue
Tsai Tai Tang
Yoko Ogata
Yoko Yamazaki
Yoshimi Yamaguchi
Yu Matsuzaki
Researcher:
Christian Schoenbach (PI)Koichi Nishiyama (PI)Motomi Osato (PI)Shinya Suzu (PI)
Takamasa Ueno (PI)
Takatsugu Ishimoto (PI)Yasuo Ariumi (PI)
Yorifumi Sato (PI)Ahad Khalil Nezhad Zein Abad
Amaya Jauregi MiguelElkhateeb Enas Hamed
Erna Rajah
Gaber Abdallah Abdallah
Hiroo Katsuya
Hiroshi Sakamoto
Itsuki Kunita
Jocelyn Yves Patrick Nicolas Turpin
Kuan Jew Win
Lingfeng Fu
Macdonald Mahiti
Madhura Subba Rao
Maiko Sezaki
Mako Toyada
Maolake Aerken
Masaya Yamazaki
Md. Fakruddin
Michihiro Hashimoto
Mikinori Ueno
Mizuho Ishikawa
Nasser Hesham Elsayed Metwally
Nguyen Thi Kim Nguyen
Osamu Noyori
Paola Miyazato
Partho Protim Adhikary
Ruslan Deviatiiarov
Sally Mehanna

Sanshiro Hanada
Shah Adil Ishtiyaq
Sho Kubota
Sofiane Hamidi
Subinoy Biswas
Takako Yokomizo
Takashi Semba
Tomomasa Yokomizo
Yasuyuki Hanada
Yoshikazu Hayashi

Student:
Amira Abdel-Daim Ibrahim Ahmed
Benjy Jek Yang Tan
Bhanubhong Prommalee
BulukeDoreen Kamori
Eita Uezono
Feng Wei
Francis Mwimanzi
Fumimasa Kitamura
George Peter Judicate Bdossi
Godfrey Barabona
Hesham Nasser
Hiroyuki Fukuda
Huaitao Wang
Isaac Ngare Akuma
Jun Zhang
Kazunori Ariyoshi
Khlood Ahmed
Khlood Khalil
Kosuke Yamada
Kota Arima
Lingfeng Fu
Madhura Subba Rao
Maiko Sezaki
Mayu Koiwa
Misaki Matsuo
Mohammad Saiful Islam
Omnia Reda Abdelsalam Abdelrahman
Ratchapon Prakongkaew
Rokeya Siddiqui
Rumi Itoyama
Sally Mehanna
Sameh Elsayed Fouad Lotfi
Saori Iwase
Sumit Sheoran
Supannika Sorin
Tadahito Yasuda
Takahiko Akiyama
Takuya Tajiri― 52 ―Tomoyuki Uchihara
Toong Seng Tan
Tsugio Eto
Wenjuan Ma
Wentao Gui
Wilan Krisna
Xichen Hu
Yang Liu
Yen Xuan Ngo
Yiling Tong
Yuechao Xia
Yuqi Sun
Yuya Okamoto

8. Appendix

8-2. List of Graduates

1.

Daisuke Izumi, PhD degree, 2016,
The mechanisms of invasiveness in gastric cancer promoted bycancer-associated fibroblasts through CXCL12/CXCR4 signaling
Ishimoto

2.

Keisuke Miyake, PhD degree, 2018,
Activation of Transforming Growth Factor Beta 1 Signaling in
Gastric Cancer-associated Fibroblasts Increases Their Motility,
via Expression of Rhomboid 5 Homolog 2, and Ability to Induce
Invasiveness of Gastric Cancer Cells
Ishimoto

14. Rumi Itoyama, PhD degree, 2022,
Metabolic shift to serine biosynthesis through 3-PG accumulation
and PHGDH induction promotes tumor growth in pancreaticcancerIshimoto
15. Buluke, PhD degree, 2023,
Tumor microenvironmental 15-PGDH depletion promotes fibrotic
tumor formation and angiogenesis in pancreatic cancer
Ishimoto
16. Feng Wei, PhD degree, 2023,
A novel tdTomato transgenic mouse model to visualize FAPpositive cancer-associated fibroblasts
Ishimoto

3.

Kota Arima, PhD degree, 2018,
Down-regulation of 15-PGDH by interleukin-1 beta from activatedmacrophages leads to poor prognosis in pancreatic cancer
Ishimoto

4.

Jew-Win Kuan, PhD degree, 2019,
Low prevalence of the BCR-ABL1 fusion gene in a normal
population in southern Sarawak.
Sashida17. Fumimasa Kitamura, PhD degree, 2023,
Cancer-associated fibroblasts reuse cancer-derived lactate tomaintain a fibrotic and immunosuppressive microenvironment inpancreatic cancer.
Ishimoto

5.

Tsugio Eto, PhD degree, 2019,
Impact of loss-of-function mutations at the RNF43 locus oncolorectal cancer development and progression
Ishimoto

18. Madhura Subba Rao, PhD degree, 2023,
Ras GTPase-activating proteins control neuronal circuit
development in barrel cortex layer 4
Mizuno6.

Tomoyuki Uchihara, PhD degree, 2020,
Extracellular Vesicles from Cancer-Associated Fibroblasts
Containing Annexin A6 Induces FAK-YAP Activation by Stabilizing
ﾀ1 Integrin, Enhancing Drug Resistance
Ishimoto

19. Takahiko Akiyama, PhD degree, 2023,
Stromal reprogramming through dual PDGFR ｿ/ ﾀ blockade
boosts the efficacy of anti-PD-1 immunotherapy in fibrotic tumors
Ishimoto

7.

Galym Ismagulov, Master’s degree, 2021,
Developing a chicken embryo-based in vivo system for analyzing
developmental potentials of hiPS cells
Sheng

8.

Maiko Sezaki, PhD degree, 2021,
CD271+CD51+PALLADIN- Human Mesenchymal Stromal Cells
Possess Enhanced Ossicle-Forming Potential
Takizawa

21. Jun Zhang, PhD degree, 2024,
IL-1 ﾀ derived from mixed-polarized macrophages activates
fibroblasts and synergistically forms a cancer-promoting
microenvironment.
Ishimoto

9.

Tadahito Yasuda, PhD degree, 2021,
Inflammation-driven senescence-associated secretory phenotype
in cancer-associated fibroblasts enhances peritoneal dissemination
Ishimoto

22. Takamitsu Egashira, Master’s degree, 2024,
Simultaneous two-photon imaging of neuronal activity andmorphology in neonatal cerebral cortex layer 4.
Mizuno10. Wenjuan Ma, PhD degree, 2021,
Folliculin regulates osteoclastogenesis through metabolic
regulation
Baba

23. Xichen Hu, PhD degree, 2024,
Downregulation of 15-PGDH enhances MASH-HCC development
via fatty acid-induced T-cell exhaustion.
Ishimoto

20. Atsuko Yonemura, PhD degree, 2024,
Mesothelial cells with mesenchymal features enhance peritoneal
dissemination by forming a protumorigenic microenvironment.
Ishimoto

11. Yuqi Su, PhD degree, 2021,
The acidic domain of Hmga2 and the domain’s linker region are
critical for driving self-renewal of hematopoietic stem cell.
Sashida12. Hiromi Mizuno, Master’s degree, 2022,
Intra-axonal mitochondria distribution in developing callosal axons
Mizuno

13. Lingfeng Fu, PhD degree, 2022,
Intracellular MUC20 variant 2 maintains mitochondrial calcium
homeostasis and enhances drug resistance in gastric cancer
Ishimoto― 53 ―

8. Appendix

8-3. List of Interns

1.

2015, Rihab Gam,Tunisia,Newcastle University, UK,Takizawa Lab

24. 2017, Noor Nabilah, Binti Talik Sisin,Malaysia,University of Sains
Malaysia,Osato Lab

2.

2015, Yu Zhang,China,Pekin University Ditan Teaching Hospital,
China,Takiguchi Lab

25. 2017, Roman Kogay,Kazakhstan,Nazarbayev University,
Kazakhstan,Shoenbach lab

3.

2015, Olexander Zhuk,Ukraine,National University of Kyiv,
Ukraine,Sheng Lab

26. 2017, Luh Ade Wilankrisna,Indonesia,Airlangga University,
Indonesia,Baba Lab

4.

2015, Benjy Jec Yang Tan,Malaysia,University Sains Malaysia,SatoLab

27. 2017, Sabera Saima,Bangladesh,Dhaka University,
Bangladesh,Takizawa Lab

5.

2015, Omnia Abdel Rahman,Egypt,High Institute of Public HealthAlexandria University, Egypt,Suzu Lab

28. 2017, Maria Veronica Saliba Pineda,Argentina,University of Buenos
Aires, Argentina,Sato Lab

6.

2015, Peter Masunga,Tanzania,Muhimbili University of Health andAllied Sciences, Tanzania,Ueno Lab

29. 2017, Galym Ismagulov,Kazakhstan,Nazarbayev University,
Kazakhstan,Schoenbach Lab

7.

2015, Hajihosseini Najaf Abadi Zohreh,Iran,Islamic Azad University,
Iran,Takizawa Lab

30. 2018, Md Shariful Islam,Bangladesh,Mawlana Bhashani Scienceand Technology University, Bangladesh,Osato Lab

8.

2015, Lilian Nkinda,Tanzania,Muhimbili University of Health andAllied Sciences, Tanzania,Ueno Lab

31. 2018, Sumit Sheoran,India,Monash University, Australia,Takizawa
Lab

9.

2016, George Peter Judicate Ndossi,Tanzania,Muhimbili University
of Health and Allied Sciences, Tanzania,Ueno Lab

32. 2018, Mohammad Umer Sharif Shohan,Bangladesh,Universit of
Dhaka, Banglash,Sato Lab

10. 2016, Amarni Thomas,New Zealand,University of Otago, New
Zealand,Osato lab

33. 2018, Nur Azrah Fazera Binti Mohd Aariffin,Malaysia,Universiti
Saints Malaysia,Suzu Lab

11. 2016, Hayaza Suhailah,Indonesia,University of Airlangga,
Indonesia,Takizawa Lab

34. 2018, Tabassum Rahman Sunfi,Bangladesh,University of Dhake,
Bangladesh,Sashida Lab

12. 2016, Ramazan Ugur Bora,Turkey,Dokuz Eylul University,
Turkey,Baba Lab

35. 2018, Madhura Subba Rao,India,Manipal University, India,MizunoLab

13. 2016, Arif, Nur Muhammad Ansori,Indonesia,Airlangga University,
Indonesia,Suzu Lab

36. 2018, Christopher, Jin Jie Chan,Singapore,University of Cambridge,
UK,Sheng Lab

14. 2016, Shekha Selemani Alinisi,Tanzania,Muhimbili University of
Health and Allied Sciences, Tanzania,Ueno Lab

37. 2018, Colver Ken HoweNe,Singapore,University of Cambridge,
UK,Ishimoto Lab

15. 2016, Md Emdad Ullah,Bangladesh,University of Dhake,
Bangladesh,Sashida Lab

38. 2018, Jieun Moon,Korea,KAIST, Korea,Nishiyama Lab

16. 2016, Soyeon Ahn,Korea,KAIST, Korea,Nishiyama Lab

39. 2019, Malini Rethnam,Singapore,National University ofSingapore,Suda Lab

17. 2016, Yong Chuan Tan,Singapore,National University ofSingapore,Suda Lab

40. 2019, Elvira Abzhanova,Kazakhstan,Nazarbayev University,
Kazakhstan,Mizuno Lab

18. 2016, Yulia Rahmawati,Indonesia,Airlangga University,
Indonesia,Sato lab

41. 2019, Aizhan Baltabay,Kazakhstan,Astana Medical University,
Kazakhstan,Sashida Lab

19. 2017, Mariyam Akizhanova,Kazakhstan,Nazarbayev University,
Kazakhstan,Sheng Lab

42. 2019, Alban, August Johansson,Sweden,Lund University,
Sweden,Takizawa Lab

20. 2017, Almira Zhantuyakova,Kazakhstan,Nazarbayev University,
Kazakhstan,Suda lab

43. 2019, Vincent, Pascal Hahaut,Belgium,University of Liege,
Belgium,Sato Lab

21. 2017, Sumaiya Zaman,Bangladesh,University of Dhake,
Bangladesh,Ariumi Lab

44. 2019, Fetemeh Mirzapourshafiyi,India,Max Planck Institute forHeart and Lung Research, Germany,Nishiyama Lab

22. 2017, Aigul Sharip,Kazakhstan,Nazarbayev University,
Kazakhstan,Schoenbach lab

45. 2019, Aidana Mustafa,Kazakhstan,Nazarbayev University,
Kazakhstan,Takizawa Lab

23. 2017, Upendo Ozeniel Kibwana,Tanzania,Muhimbili University of
Health and Allied Sciences, Tanzania,Ueno Lab

46. 2019, Michael, John Casey,Ireland,University of Southampton,
UK,Suda Lab― 54 ―

47. 2019, Nazia Islam,Bangladesh,Nazarbayev University,
Kazakhstan,Sheng Lab
48. 2019, Aiganym Abduraimova,Kazakhstan,Nazarbayev University,
Kazakhstan,Sheng Lab
49. 2020, Shiv Jha,Nepal,Tribhuvan University, Nepal,Suda Lab

50. 2020, Md Naeem Hossain,Bangladesh,University of Dhake,
Bangladesh,Takizawa Lab
51. 2022, Abdullah Baransel Yalcin,Turkey,Mersin University Health
Sciences Institute Stem Cell ang Regenerative, Turkey,Suda Lab
52. 2022, Baby Naznin,Bangladesh,University of Dhaka,Bangladesh,Baba Lab
53. 2022, Mai Abdelgawad,Egypt,Suez Canal University, Egypt,Sada
Lab
54. 2022, Katharina Miriam Schonberger,Germany,Albert-Ludwigs
University Freiburg,Takizawa Lab
55. 2022, Raikhangul Gabdrashova,Kazakhstan,University of Turin,
Italy,Mizuno Lab
56. 2022, Azamat Akhmetkaliyev,Kazakhstan,Nazarbayev University,
Kazakhstan,Suda Lab
57. 2022, Arusyak Ivanyan,Armenia,Yerevan State Medical
University,Sashida Lab
58. 2023, En Chi Lai,Taiwan,Cardiff University, UK,Takizawa Lab

59. 2023, Gaukhar Mukash,Kazakhstan,University of Eastern
Finland,Miharada Lab
60. 2023, Rina Iwata,Japan,University of Chicago, USA,Suda Lab

61. 2023, Teona Paresishvili,Georgia,Tbilisi State Medical University,
Georgia,Sashida Lab
62. 2023, Kulkarni Shreya,India,Indian Institute of Science Education
and Research, Pune,Sheng Lab
63. 2023, Sakib Mohammad Nazmus,Bangladesh,Industrial
Biotechnology Lab, Jahangirnagar University, Bangladesh,KurotakiLab
64. 2023, Kumar Gaurav,India,Indian Institute of Science Education
and Research, Bhopal,Sheng Lab
65. 2023, Antonius Yulanda,Indonesia,University of Surabaya,
Indonesia,Miharada Lab
66. 2023, Bassey Esther Edet,Nigeria,University of Uyo, Nigeria,Arima
Lab― 55 ―

8. Appendix

8-4. List of Publications

1.

CLEC-2 in megakaryocytes is critical for maintenance ofhematopoietic stem cells in the bone marrow
Nakamura-Ishizu, Ayako; Takubo, Keiyo; Kobayashi, Hiroshi;
Suzuki-Inoue, Katsue; Suda, Toshio
JOURNAL OF EXPERIMENTAL MEDICINE 212.12:21332146,2015 MEDLINE:26552707 Article

2.

Autonomy and Non-autonomy of Angiogenic Cell Movements
Revealed by Experiment-Driven Mathematical Modeling
Sugihara, Kei; Nishiyama, Koichi; Fukuhara, Shigetomo; Uemura,
Akiyoshi; Arima, Satoshi; Kobayashi, Ryo; Koehn-Luque, Alvaro;
Mochizuki, Naoki; Suda, Toshio; Ogawa, Hisao; Kurihara, Hiroki
CELL REPORTS 13.9:1814-1827,2015 MEDLINE:26655898 Article

3.

The developmental basis of mesenchymal stem/stromal cells(MSCs)
Sheng, Guojun
BMC DEVELOPMENTAL BIOLOGY 15.n/a:n/a,2015
MEDLINE:26589542 Article

4.

Mouse models for core binding factor leukemia
Chin, D. W. L.; Watanabe-Okochi, N.; Wang, C. Q.; Tergaonkar, V.;
Osato, M.
LEUKEMIA 29.10:1970-1980,2015 MEDLINE:26165235 Review

5.

Aspp1 Preserves Hematopoietic Stem Cell Pool Integrity andPrevents Malignant Transformation
Yamashita, Masayuki; Nitta, Eriko; Suda, Toshio
CELL STEM CELL 17.1:23-34,2015 MEDLINE:26119234 Article

6.

Ezh2 loss in hematopoietic stem cells predisposes mice todevelop heterogeneous malignancies in an Ezh1-dependent
mannerMochizuki-Kashio, Makiko; Aoyama, Kazumasa; Sashida, Goro;
Oshima, Motohiko; Tomioka, Takahisa; Muto, Tomoya; Wang,
Changshan; Iwama, Atsushi
BLOOD 126.10:1172-1183,2015 MEDLINE:26219303 Article

7.

Integrative Analysis of the Acquisition of Pluripotency in
PGCs Reveals the Mutually Exclusive Roles of Blimp-I andAKT Signaling
Nagamatsu, Go; Saito, Shigeru; Takubo, Keiyo; Suda, Toshio
STEM CELL REPORTS 5.1:111-124,2015 MEDLINE:26050930
Article8.

Non-Lethal Ionizing Radiation Promotes Aging-Like
Phenotypic Changes of Human Hematopoietic Stem andProgenitor Cells in Humanized MiceWang, Changshan; Oshima, Motohiko; Sashida, Goro; Tomioka,
Takahisa; Hasegawa, Nagisa; Mochizuki-Kashio, Makiko;
Nakajima-Takagi, Yaeko; Kusunoki, Yoichiro; Kyoizumi, Seishi; Imai,
Kazue; Nakachi, Kei; Iwama, Atsushi
PLOS ONE 10.7:n/a,2015 MEDLINE:26161905 Article9.

miR-9-3p plays a tumour-suppressor role by targeting TAZ(WWTR1) in hepatocellular carcinoma cells
Higashi, T.; Hayashi, H.; Ishimoto, T.; Takeyama, H.; Kaida, T.;
Arima, K.; Taki, K.; Sakamoto, K.; Kuroki, H.; Okabe, H.; Nitta, H.;
Hashimoto, D.; Chikamoto, A.; Beppu, T.; Baba, H.
BRITISH JOURNAL OF CANCER 113.2:252-258,2015
MEDLINE:26125451 Article

10. Analysis of circulating tumor cells derived from advanced
gastric cancer
Toyoshima, Kosei; Hayashi, Akira; Kashiwagi, Masahide; Hayashi,
Naoko; Iwatsuki, Masaaki; Ishimoto, Takatsugu; Baba, Yoshifumi;Baba, Hideo; Ohta, Yoshikazu
INTERNATIONAL JOURNAL OF CANCER 137.4:991-998,2015
MEDLINE:25622566 Article

11. Fbxl10 overexpression in murine hematopoietic stem
cells induces leukemia involving metabolic activation andupregulation of Nsg2
Ueda, Takeshi; Nagamachi, Akiko; Takubo, Keiyo; Yamasaki,
Norimasa; Matsui, Hirotaka; Kanai, Akinori; Nakata, Yuichiro; Ikeda,
Kenichiro; Konuma, Takaaki; Oda, Hideaki; Wolff, Linda; Honda,
Zen-ichiro; Wu, Xudong; Helin, Kristian; Iwama, Atsushi; Suda,
Toshio; Inaba, Toshiya; Honda, Hiroaki
BLOOD 125.22:3437-3446,2015 MEDLINE:25872778 Article
12. Hematopoietic stem cell enhancer: a powerful tool in stem
cell biology
Koh, Cai Ping; Ng, Cherry Ee Lin; Nah, Giselle Sek Suan; Wang,
Chelsia Qiuxia; Tergaonkar, Vinay; Matsumura, Takayoshi;
Yokomizo, Tomomasa; Suda, Toshio; Osato, Motomi
HISTOLOGY AND HISTOPATHOLOGY 30.6:661-672,2015
MEDLINE:25574754 Review
13. Proliferation Following Tetraploidization Regulates the Sizeand Number of Erythrocytes in the Blood Flow During Medaka
Development, as Revealed by the Abnormal Karyotype ofErythrocytes in the Medaka TFDP1 Mutant
Taimatsu, Kiyohito; Takubo, Keiyo; Maruyama, Kouichi; Suda,
Toshio; Kudo, Akira
DEVELOPMENTAL DYNAMICS 244.5:651-668,2015
MEDLINE:25648602 Article
14. Bacterial c-di-GMP Affects Hematopoietic Stem/Progenitors
and Their Niches through STING
Kobayashi, Hiroshi; Kobayashi, Chiharu I.; Nakamura-Ishizu, Ayako;
Karigane, Daiki; Haeno, Hiroshi; Yamamoto, Kimiyo N.; Sato, Taku;
Ohteki, Toshiaki; Hayakawa, Yoshihiro; Barber, Glen N.; Kurokawa,
Mineo; Suda, Toshio; Takubo, Keiyo
CELL REPORTS 11.1:71-84,2015 MEDLINE:25843711 Article
15. Folliculin-interacting proteins Fnip1 and Fnip2 play criticalroles in kidney tumor suppression in cooperation with Flcn
Hasumi, Hisashi; Baba, Masaya; Hasumi, Yukiko; Lang, Martin;
Huang, Ying; Oh, Hyoungbin F.; Matsuo, Masayuki; Merino, MariaJ.; Yao, Masahiro; Ito, Yusuke; Furuya, Mitsuko; Iribe, Yasuhiro;
Kodama, Tatsuhiko; Southon, Eileen; Tessarollo, Lino; Nagashima,
Kunio; Haines, Diana C.; Linehan, W. Marston; Schmidt, Laura S.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES
OF THE UNITED STATES OF AMERICA 112.13:E1624-E1631,2015
MEDLINE:25775561 Article
16. The sensitivity of gastric cancer to trastuzumab is regulatedby the miR-223/FBXW7 pathway
Eto, Kojiro; Iwatsuki, Masaaki; Watanabe, Masayuki; Ishimoto,Takatsugu; Ida, Satoshi; Imamura, Yu; Iwagami, Shiro; Baba,
Yoshifumi; Sakamoto, Yasuo; Miyamoto, Yuji; Yoshida, Naoya;
Baba, Hideo
INTERNATIONAL JOURNAL OF CANCER 136.7:1537-1545,2015
MEDLINE:25159729 Article
17. Determining c-Myb Protein Levels Can Isolate Functional
Hematopoietic Stem Cell Subtypes
Sakamoto, Hiroshi; Takeda, Naoki; Arai, Fumio; Hosokawa,
Kentaro; Garcia, Paloma; Suda, Toshio; Frampton, Jon; Ogawa,
Minetaro
STEM CELLS 33.2:479-490,2015 MEDLINE:25329760 Article
18. 2016 update on APBioNet’s annual international conferenceon bioinformatics (InCoB)
Schonbach, Christian; Verma, Chandra; Wee, Lawrence Jin Kiat;
Bond, Peter John; Ranganathan, Shoba
BMC GENOMICS 17.n/a:n/a,2016 MEDLINE:28155656 Article― 56 ―

19. Bioinformatics and systems biology research update from the15th International Conference on Bioinformatics (InCoB2016)
Schonbach, Christian; Verma, Chandra; Bond, Peter J.;
Ranganathan, Shoba
BMC BIOINFORMATICS 17.n/a:n/a,2016 MEDLINE:28155668
Article20. Self-renewal of a purified Tie2(+) hematopoietic stem cell
population relies on mitochondrial clearance
Ito, Kyoko; Turcotte, Raphael; Cui, Jinhua; Zimmerman, Samuel E.;
Pinho, Sandra; Mizoguchi, Toshihide; Arai, Fumio; Runnels, JudithM.; Alt, Clemens; Teruya-Feldstein, Julie; Mar, Jessica C.; Singh,
Rajat; Suda, Toshio; Lin, Charles P.; Frenette, Paul S.; Ito, Keisuke
SCIENCE 354.6316:1156-1160,2016 MEDLINE:27738012 Article
21. Human Microbiome Fusobacterium Nucleatum in Esophageal
Cancer Tissue Is Associated with Prognosis
Yamamura, Kensuke; Baba, Yoshifumi; Nakagawa, Shigeki;Mima, Kosuke; Miyake, Keisuke; Nakamura, Kenichi; Sawayama,
Hiroshi; Kinoshita, Koichi; Ishimoto, Takatsugu; Iwatsuki, Masaaki;
Sakamoto, Yasuo; Yamashita, Yoichi; Yoshida, Naoya; Watanabe,
Masayuki; Baba, Hideo
CLINICAL CANCER RESEARCH 22.22:5574-5581,2016
MEDLINE:27769987 Article
22. MPL expression on AML blasts predicts peripheral bloodneutropenia and thrombocytopenia
Rauch, Philipp J.; Ellegast, Jana M.; Widmer, Corinne C.; Fritsch,
Kristin; Goede, Jeroen S.; Valk, Peter J. M.; Loewenberg, Bob;
Takizawa, Hitoshi; Manz, Markus G.
BLOOD 128.18:2253-2257,2016 MEDLINE:27574191 Article
23. Inflamm-Aging of Hematopoiesis, Hematopoietic Stem Cells
and the Bone Marrow Microenvironment.
Kovtonyuk LV, Fritsch K, Feng X, Manz MG, Takizawa H*.2016
24. Genetic, epidemiologic and clinicopathologic studies ofJapanese Asian patients with Birt-Hogg-Dube syndrome
Furuya, Mitsuko; Yao, Masahiro; Tanaka, Reiko; Nagashima, Yoji;
Kuroda, Naoto; Hasumi, Hisashi; Baba, Masaya; Matsushima, Jun;
Nomura, Fumio; Nakatani, Yukio
CLINICAL GENETICS 90.5:403-412,2016 MEDLINE:27220747
Article25. Setdb1 maintains hematopoietic stem and progenitor cells by
restricting the ectopic activation of nonhematopoietic genes
Koide, Shuhei; Oshima, Motohiko; Takubo, Keiyo; Yamazaki,
Satoshi; Nitta, Eriko; Saraya, Atsunori; Aoyama, Kazumasa; Kato,
Yuko; Miyagi, Satoru; Nakajima-Takagi, Yaeko; Chiba, Tetsuhiro;
Matsui, Hirotaka; Arai, Fumio; Suzuki, Yutaka; Kimura, Hiroshi;
Nakauchi, Hiromitsu; Suda, Toshio; Shinkai, Yoichi; Iwama, Atsushi
BLOOD 128.5:638-649,2016 MEDLINE:27301860 Article
26. p38 alpha Ac tiva tes Purine Me tabolism to Initia te
Hematopoietic Stem/Progenitor Cell Cycling in Response to
Stress
Karigane, Daiki; Kobayashi, Hiroshi; Morikawa, Takayuki; Ootomo,
Yukako; Sakai, Mashito; Nagamatsu, Go; Kubota, Yoshiaki;
Goda, Nobuhito; Matsumoto, Michihiro; Nishimura, Emi K.; Soga,
Tomoyoshi; Otsu, Kinya; Suematsu, Makoto; Okamoto, Shinichiro;Suda, Toshio; Takubo, Keiyo
CELL STEM CELL 19.2:192-204,2016 MEDLINE:27345838 Article
27. The loss of Ezh2 drives the pathogenesis of myelofibrosis
and sensitizes tumor-initiating cells to bromodomain
inhibition
Sashida, Goro; Wang, Changshan; Tomioka, Takahisa; Oshima,
Motohiko; Aoyama, Kazumasa; Kanai, Akinori; Mochizuki-Kashio,
Makiko; Harada, Hironori; Shimoda, Kazuya; Iwama, Atsushi
JOURNAL OF EXPERIMENTAL MEDICINE 213.8:14591477,2016 MEDLINE:27401345 Article

28. Repopulation dynamics of single haematopoietic stem cells
in mouse transplantation experiments: Importance of stem
cell composition in competitor cells
Ema, Hideo; Uchinomiya, Kouki; Morita, Yohei; Suda, Toshio; Iwasa,
Yoh
JOURNAL OF THEORETICAL BIOLOGY 394.n/a:57-67,2016
MEDLINE:26802482 Article
29. Maintenanc e o f the func tional integrit y o f mousehematopoiesis by EED and promotion of leukemogenesis by
EED haploinsufficiency
Ikeda, Kenichiro; Ueda, Takeshi; Yamasaki, Norimasa; Nakata,
Yuichiro; Sera, Yasuyuki; Nagamachi, Akiko; Miyama, Takahiko;
Kobayashi, Hiroshi; Takubo, Keiyo; Kanai, Akinori; Oda, Hideaki;
Wolff, Linda; Honda, Zen-ichiro; Ichinohe, Tatsuo; Matsubara, Akio;
Suda, Toshio; Inaba, Toshiya; Honda, Hiroaki
SCIENTIFIC REPORTS 6.n/a:n/a,2016 MEDLINE:27432459 Article
30. Regulation of hematopoietic stem cell integrity through p53
and its related factors
Yamashita, Masayuki; Nitta, Eriko; Suda, Toshio
HEMATOPOIETIC STEM CELLS IX 1370.n/a:45-54,2016
MEDLINE:26695737 Article

31. Human adipose tissue-derived stromal cells can differentiate
into megakaryocytes and platelets by secreting endogenous
thrombopoietin
Ono-Uruga, Y.; Tozawa, K.; Horiuchi, T.; Murata, M.; Okamoto, S.;
Ikeda, Y.; Suda, T.; Matsubara, Y.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS 14.6:12851297,2016 MEDLINE:26990635 Article
32. Enhanced thrombopoietin but not G-CSF receptor stimulationinduces self-renewing hematopoietic stem cell divisions invivo
Kovtonyuk, Larisa V.; Manz, Markus G.; Takizawa, Hitoshi
BLOOD 127.25:3175-3179,2016 MEDLINE:27146433 Article
33. Effects of a Single EscapeMutation on T Cell and HIV-1 Coadaptation
Sun, Xiaoming; Shi, Yi; Akahoshi, Tomohiro; Fujiwara, Mamoru;
Gatanaga, Hiroyuki; Schonbach, Christian; Kuse, Nozomi; Appay,
Victor; Gao, George F.; Oka, Shinichi; Takiguchi, Masafumi
CELL REPORTS 15.10:2279-2291,2016 MEDLINE:27239036
Article34. Lack of Phenotypical and Morphological Evidences ofEndothelial to Hematopoietic Transition in the Murine
Embryonic Head during Hematopoietic Stem Cell Emergence
Iizuka, Kazuhide; Yokomizo, Tomomasa; Watanabe, Naoki; Tanaka,
Yosuke; Osato, Motomi; Takaku, Tomoiku; Komatsu, Norio
PLOS ONE 11.5:n/a,2016 MEDLINE:27227884 Article
35. Flt1/VEGFR1 heterozygosity causes transient embryonic
edema
Otowa, Yasunori; Moriwaki, Kazumasa; Sano, Keigo; Shirakabe,
Masanori; Yonemura, Shigenobu; Shibuya, Masabumi; Rossant,Janet; Suda, Toshio; Kakeji, Yoshihiro; Hirashima, Masanori
SCIENTIFIC REPORTS 6.n/a:n/a,2016 MEDLINE:27251772 Article
36. Epigenetic field cancerization in gastrointestinal cancers
Baba, Yoshifumi; Ishimoto, Takatsugu; Kurashige, Junji; Iwatsuki,
Masaaki; Sakamoto, Yasuo; Yoshida, Naoya; Watanabe, Masayuki;
Baba, Hideo
CANCER LETTERS 375.2:360-366,2016 MEDLINE:26971491
Review― 57 ―

37. Chronic AMPK activation via loss of FLCN induces functional
beige adipose tissue through PGC-1 alpha/ERR alphaYan, Ming; Audet-Walsh, Etienne; Manteghi, Sanaz; Dufour,
Catherine Rosa; Walker, Benjamin; Baba, Masaya; St-Pierre, Julie;
Giguere, Vincent; Pause, Arnim
GENES & DEVELOPMENT 30.9:1034-1046,2016
MEDLINE:27151976 Article
38. Assessment of platelet-derived thrombogenicity with thetotal thrombus-formation analysis system in coronary arterydisease patients receiving antiplatelet therapy
Arima, Y.; Kaikita, K.; Ishii, M.; Ito, M.; Sueta, D.; Oimatsu, Y.;
Sakamoto, K.; Tsujita, K.; Kojima, S.; Nakagawa, K.; Hokimoto, S.;
Ogawa, H.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS 14.4:850859,2016 MEDLINE:26773298 Article
39. Loss of Folliculin Disrupts Hematopoietic Stem Cell
Quiescence and Homeostasis Resulting in Bone Marrow
FailureBaba, Masaya; Toyama, Hirofumi; Sun, Lei; Takubo, Keiyo; Suh,
Hyung-Chan; Hasumi, Hisashi; Nakamura-Ishizu, Ayako; Hasumi,
Yukiko; Klarmann, Kimberly D.; Nakagata, Naomi; Schmidt, LauraS.; Linehan, W. Marston; Suda, Toshio; Keller, Jonathan R.
STEM CELLS 34.4:1068-1082,2016 MEDLINE:27095138 Article
40. Birt-Hogg-Dube syndrome: Clinical and molecular aspects ofrecently identified kidney cancer syndrome
Hasumi, Hisashi; Baba, Masaya; Hasumi, Yukiko; Furuya, Mitsuko;
Yao, Masahiro
INTERNATIONAL JOURNAL OF UROLOGY 23.3:204-210,2016
MEDLINE:26608100 Review
41. Current perspectives toward the identification of key players
in gastric cancer microRNA dysregulation
Ishimoto, Takatsugu; Baba, Hideo; Izumi, Daisuke; Sugihara,
Hidetaka; Kurashige, Junji; Iwatsuki, Masaaki; Tan, Patrick
INTERNATIONAL JOURNAL OF CANCER 138.6:1337-1349,2016
MEDLINE:26041092 Review
42. How hematopoietic stem/progenitors and their niche sense
and respond to infectious stress
Kobayashi, Hiroshi; Suda, Toshio; Takubo, Keiyo
EXPERIMENTAL HEMATOLOGY 44.2:92-100,2016
MEDLINE:26646990 Review

45. REVIEW OF HEREDITARY LEIOMYOMATOSIS RENALCELL CARCINOMA WITH FOCUS ON CLINICAL AND
PATHOBIOLOGICAL ASPECTS OF RENAL TUMORS
Kuroda, Naoto; Ohe, Chisato; Kato, Ikuma; Furuya, Mitsuko; Baba,
Masaya; Nagashima, Yoji; Nakatani, Yukio; Murakami, Ichiro; Zhou,
Ming; Michal, Michal; He, Ondrej; Amin, Mahul B.
POLISH JOURNAL OF PATHOLOGY 68.4:284-290,2017
MEDLINE:29517197 Review
46. Reactive Oxygen Species and Mitochondrial Homeostasis as
Regulators of Stem Cell Fate and FunctionTan, Darren Q.; Suda, Toshio
ANTIOXIDANTS & REDOX SIGNALING 29.2:149-168,2017
MEDLINE:28708000 Review
47. Mfsd2b is essential for the sphingosine-1-phosphate export inerythrocytes and platelets
Vu, Thiet M.; Ishizu, Ayako-Nakamura; Foo, Juat Chin; Xiu Ru Toh;
Zhang, Fangyu; Whee, Ding Ming; Torta, Federico; CazenaveGassiot, Amaury; Matsumura, Takayoshi; Kim, Sangho; Toh,
Sue-Anne E. S.; Suda, Toshio; Silver, David L.; Wenk, Markus R.;
Nguyen, Long N.
NATURE 550.7677:524-+,2017 MEDLINE:29045386 Article
48. A DNA Contact Map for the Mouse Runx1 Gene Identifies
Novel Haematopoietic Enhancers
Marsman, Judith; Thomas, Amarni; Osato, Motomi; O’Sullivan,
Justin M.; Horsfield, Julia A.
SCIENTIFIC REPORTS 7.n/a:n/a,2017 MEDLINE:29042628 Article
49. In Vivo Generation of Engraftable Murine Hematopoietic
Stem Cells by Gfi1b, c-Fos, and Gata2 Overexpression within
Teratoma
Tsukada, Masao; Ota, Yasunori; Wilkinson, Adam C.; Becker, Hans
J.; Osato, Motomi; Nakauchi, Hiromitsu; Yamazaki, Satoshi
STEM CELL REPORTS 9.4:1024-1033,2017 MEDLINE:28943250
Article50. The telomere binding protein Pot1 maintains haematopoietic
stem cell activity with age
Hosokawa, Kentaro; MacArthur, Ben D.; Ikushima, YoshikoMatsumoto; Toyama, Hirofumi; Masuhiro, Yoshikazu; Hanazawa,
Shigemasa; Suda, Toshio; Arai, Fumio
NATURE COMMUNICATIONS 8.n/a:n/a,2017 MEDLINE:28986560
Article43. CXCL12/CXCR4 activation by cancer-associated fibroblasts
promotes integrin beta 1 clustering and invasiveness ingastric cancer
Izumi, Daisuke; Ishimoto, Takatsugu; Miyake, Keisuke; Sugihara,
Hidetaka; Eto, Kojiro; Sawayama, Hiroshi; Yasuda, Tadahito;
Kiyozumi, Yuki; Kaida, Takayoshi; Kurashige, Junji; Imamura,Yu; Hiyoshi, Yukiharu; Iwatsuki, Masaaki; Iwagami, Shiro; Baba,
Yoshifumi; Sakamoto, Yasuo; Miyamoto, Yuji; Yoshida, Naoya;
Watanabe, Masayuki; Takamori, Hiroshi; Araki, Norie; Tan, Patrick;Baba, Hideo
INTERNATIONAL JOURNAL OF CANCER 138.5:1207-1219,2016
MEDLINE:26414794 Article

51. Runx Family Genes in Tissue Stem Cell Dynamics
Wang, Chelsia Qiuxia; Mok, Michelle Meng Huang; Yokomizo,
Tomomasa; Tergaonkar, Vinay; Osato, Motomi
RUNX PROTEINS IN DEVELOPMENT AND CANCER 962.n/
a:117-138,2017 MEDLINE:28299655 Article

44. Lysine-specific demethylase-1 contributes to malignant
behavior by regulation of invasive activity and metabolic shift
in esophageal cancer
Kosumi, Keisuke; Baba, Yoshifumi; Sakamoto, Akihisa; Ishimoto,Takatsugu; Harada, Kazuto; Nakamura, Kenichi; Kurashige, Junji;
Hiyoshi, Yukiharu; Iwatsuki, Masaaki; Iwagami, Shiro; Sakamoto,Yasuo; Miyamoto, Yuji; Yoshida, Naoya; Oki, Eiji; Watanabe,
Masayuki; Hino, Shinjiro; Nakao, Mitsuyoshi; Baba, Hideo
INTERNATIONAL JOURNAL OF CANCER 138.2:428-439,2016
MEDLINE:26240060 Article

53. Systematic analysis of transcription start sites in avian
development
Lizio, Marina; Deviatiiarov, Ruslan; Nagai, Hiroki; Galan, Laura;
Arner, Erik; Itoh, Masayoshi; Lassmann, Timo; Kasukawa, Takeya;
Hasegawa, Akira; Ros, Marian A.; Hayashizaki, Yoshihide; Carninci,
Piero; Forrest, Alistair R. R.; Kawaji, Hideya; Gusev, Oleg; Sheng,
GuojunPLOS BIOLOGY 15.9:n/a,2017 MEDLINE:28873399 Article

52. RUNX3 is oncogenic in natural killer/T-cell lymphoma and istranscriptionally regulated by MYC
Selvarajan, V.; Osato, M.; Nah, G. S. S.; Yan, J.; Chung, T-H; Voon,
D. C-C; Ito, Y.; Ham, M. F.; Salto-Tellez, M.; Shimizu, N.; Choo, S-N;
Fan, S.; Chng, W-J; Ng, S-B
LEUKEMIA 31.10:2219-2227,2017 MEDLINE:28119527 Article― 58 ―

54. Colorectal Cancer Stem Cells Acquire ChemoresistanceThrough the Upregulation of F-Box/WD Repeat-Containing
Protein 7 and the Consequent Degradation of c-Myc
Izumi, Daisuke; Ishimoto, Takatsugu; Miyake, Keisuke; Eto, Tsugio;
Arima, Kota; Kiyozumi, Yuki; Uchihara, Tomoyuki; Kurashige, Junji;
Iwatsuki, Masaaki; Baba, Yoshifumi; Sakamoto, Yasuo; Miyamoto,Yuji; Yoshida, Naoya; Watanabe, Masayuki; Goel, Ajay; Tan,
Patrick; Baba, Hideo
STEM CELLS 35.9:2027-2036,2017 MEDLINE:28699179 Article
55. Integrin alpha v beta 3 enhances the suppressive effect ofinterferon-gamma on hematopoietic stem cells
Umemoto, Terumasa; Matsuzaki, Yu; Shiratsuchi, Yoshiko;Hashimoto, Michihiro; Yoshimoto, Takayuki; Nakamura-Ishizu,
Ayako; Petrich, Brian; Yamato, Masayuki; Suda, Toshio
EMBO JOURNAL 36.16:2390-2403,2017 MEDLINE:28673932
Article56. Pathogen-Induced TLR4-TRIF Innate Immune Signaling in
Hematopoietic Stem Cells Promotes Proliferation but Reduces
Competitive Fitness
Takizawa, Hitoshi; Fritsch, Kristin; Kovtonyuk, Larisa V.; Saito,
Yasuyuki; Yakkala, Chakradhar; Jacobs, Kurt; Ahuja, Akshay
K.; Lopes, Massimo; Hausmann, Annika; Hardt, Wolf-Dietrich;
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60. Activation of Transforming Growth Factor Beta 1 Signaling
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77. Pathobiological Pseudohypoxia as a Putative Mechanism
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107.TFE3 Xp11.2 Translocation Renal Cell Carcinoma Mouse Model
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Hisashi; Huang, Ying; Kato, Ikuma; Kadomatsu, Tsuyoshi; Satou,
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Aiko; Kurahashi, Ryoma; Nishimoto, Koshiro; Oyama, Masafumi;
Nagashima, Yoji; Kuroda, Naoto; Araki, Kimi; Eto, Masatoshi; Yao,
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108.Can PD-L1 expression evaluated by biopsy sample accuratelyreflect its expression in the whole tumour in gastric cancer?
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Kiyozumi, Yuki; Eto, Kojiro; Hiyoshi, Yukiharu; Ishimoto, Takatsugu;
Iwagami, Shiro; Baba, Yoshifumi; Miyamoto, Yuji; Yoshida, Naoya;
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BRITISH JOURNAL OF CANCER 121.3:278-280,2019
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109.Multifaceted roles of thrombopoietin in hematopoietic stem
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110.RBM10-TFE3 renal cell carcinoma characterised byparacentric inversion with consistent closely split signals in
break-apart fluorescence in-situ hybridisation: study of 10
cases and a literature review
Kato, Ikuma; Furuya, Mitsuko; Baba, Masaya; Kameda, Yoichi;
Yasuda, Masanori; Nishimoto, Koshiro; Oyama, Masafumi;
Yamasaki, Toshinari; Ogawa, Osamu; Niino, Hitoshi; Nakaigawa,
Noboru; Yano, Yuta; Sakamoto, Kazumasa; Urata, Yoji; Mikami,
Kazuya; Yamasaki, Shigetaka; Tanaka, Reiko; Takagi, Toshio;
Kondo, Tsunenori; Nagashima, Yoji
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111.Deregulated Polycomb functions in myeloproliferative
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112.ISM1 regulates NODAL signaling and asymmetric organ
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JOURNAL OF CELL BIOLOGY 218.7:2388-2402,2019
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113.Hlf marks the developmental pathway for hematopoietic stem
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Yokomizo, Tomomasa; Watanabe, Naoki; Umemoto, Terumasa;
Matsuo, Junichi; Harai, Ryota; Kihara, Yoshihiko; Nakamura, Eri;
Tada, Norihiro; Sato, Tomohiko; Takaku, Tomoiku; Shimono, Akihiko;
Takizawa, Hitoshi; Nakagata, Naomi; Mori, Seiichi; Kurokawa,
Mineo; Tenen, Daniel G.; Osato, Motomi; Suda, Toshio; Komatsu,
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114.MicroRNA-204-5p: A novel candidate urinary biomarker of
Xp11.2 translocation renal cell carcinoma
Kurahashi, Ryoma; Kadomatsu, Tsuyoshi; Baba, Masaya; Hara,
Chiaki; Itoh, Hitoshi; Miyata, Keishi; Endo, Motoyoshi; Morinaga,
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Article115.TUBB1 dysfunction in inherited thrombocytopenia causes
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BRITISH JOURNAL OF HAEMATOLOGY 185.5:888-902,2019
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116.Biological heterogeneity and versatility of cancer-associatedfibroblasts in the tumor microenvironment
Bu, Luke; Baba, Hideo; Yoshida, Naoya; Miyake, Keisuke; Yasuda,
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ONCOGENE 38.25:4887-4901,2019 MEDLINE:30816343 Review
117.Development of the hematopoietic system: Role ofinflammatory factors
Hayashi, Yoshikazu; Sezaki, Maiko; Takizawa, Hitoshi
WILEY INTERDISCIPLINARY REVIEWS-DEVELOPMENTAL
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118.Evolution of the avian digital pattern
Kawahata, Kenta; Cordeiro, Ingrid Rosenburg; Ueda, Shogo;
Sheng, Guojun; Moriyama, Yuuta; Nishimori, Chika; Yu, Reiko;Koizumi, Makoto; Okabe, Masataka; Tanaka, Mikiko
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119.PD-L1 Expression, Tumor-infiltrating Lymphocytes, andClinical Outcome in Patients With Surgically Resected
Esophageal Cancer
Yagi, Taisuke; Baba, Yoshifumi; Ishimoto, Takatsugu; Iwatsuki,
Masaaki; Miyamoto, Yuji; Yoshida, Naoya; Watanabe, Masayuki;
Baba, Hideo
ANNALS OF SURGERY 269.3:471-478,2019 MEDLINE:29206673
Article120.B i o m e c h a ni c a l r e g ul a t i o n o f E M T a n d e p i t h e li a lmorphogenesis in amniote epiblast
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PHYSICAL BIOLOGY 16.4:n/a,2019 MEDLINE:30875695 Review
121.A genomewide transcriptomic approach identifies a novel
gene expression signature for the detection of lymph nodemetastasis in patients with early stage gastric cancer
Izumi, Daisuke; Gao, Feng; Toden, Shusuke; Sonohara, Fuminori;
Kanda, Mitsuro; Ishimoto, Takatsugu; Kodera, Yasuhiro; Wang, Xin;
Baba, Hideo; Goel, Ajay
EBIOMEDICINE 41.n/a:268-275,2019 MEDLINE:30772302 Article
122.Sphingosine-1-phosphate signaling modulates terminal
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Yang, Chong; Hashimoto, Michihiro; Lin, Quy Xiao Xuan; Tan,
Darren Qiancheng; Suda, Toshio
EXPERIMENTAL HEMATOLOGY 72.n/a:47-59,2019
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123.Lineage-specific RUNX2 super-enhancer activates MYC andpromotes the development of blastic plasmacytoid dendritic
cell neoplasm
Kubota, Sho; Tokunaga, Kenji; Umezu, Tomohiro; YokomizoNakano, Takako; Sun, Yuqi; Oshima, Motohiko; Tan, Kar Ton; Yang,Henry; Kanai, Akinori; Iwanaga, Eisaku; Asou, Norio; Maeda,
Takahiro; Nakagata, Naomi; Iwama, Atsushi; Ohyashiki, Kazuma;
Osato, Motomi; Sashida, Goro
NATURE COMMUNICATIONS 10.n/a:n/a,2019
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124.TIAM1 promotes chemoresistance and tumor invasiveness incolorectal cancer
Izumi, Daisuke; Toden, Shusuke; Ureta, Elsie; Ishimoto, Takatsugu;
Baba, Hideo; Goel, Ajay
CELL DEATH & DISEASE 10.n/a:n/a,2019 MEDLINE:30890693
Article125.Tumour-associated macrophages are associated with poor
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Miyamoto, Yuji; Yoshida, Naoya; Watanabe, Masayuki; Komohara,
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EUROPEAN JOURNAL OF CANCER 111.n/a:38-49,2019
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126.PRMT5 Modulates Splicing for Genome Integrity and
Preserves Proteostasis of Hematopoietic Stem Cells
Tan, Darren Qiancheng; Li, Ying; Yang, Chong; Li, Jia; Tan, Shi Hao;
Chin, Desmond Wai Loon; Nakamura-Ishizu, Ayako; Yang, Henry;Suda, Toshio
CELL REPORTS 26.9:2316-+,2019 MEDLINE:30811983 Article
127.Inhibition of 15-PGDH causes Kras-driven tumor expansion
through prostaglandin E2-ALDH1 signaling in the pancreas
Arima, Kota; Ohmuraya, Masaki; Miyake, Keisuke; Koiwa, Mayu;
Uchihara, Tomoyuki; Izumi, Daisuke; Gao, Feng; Yonemura, Atsuko;
Bu, Luke; Okabe, Hirohisa; Imai, Katsunori; Hashimoto, Daisuke;
Baba, Yoshifumi; Chikamoto, Akira; Yamashita, Yo-ichi; Furukawa,
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ONCOGENE 38.8:1211-1224,2019 MEDLINE:30250298 Article
128.Modification of the bone marrow MSC population in a
xenograft model of early multiple myeloma
Berlier, Jessica L.; Rethnam, Malini; Majeed, A’Qilah Banu Binte
Abdul; Suda, Toshio
BIOCHEMICAL AND BIOPHYSICAL RESEARCHCOMMUNICATIONS 508.4:1175-1181,2019 MEDLINE:30553454
Article129.Progress in characterizing the linkage between Fusobacterium
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Liu, Yang; Baba, Yoshifumi; Ishimoto, Takatsugu; Iwatsuki, Masaaki;
Hiyoshi, Yukiharu; Miyamoto, Yuji; Yoshida, Naoya; Wu, Rong;Baba, Hideo
JOURNAL OF GASTROENTEROLOGY 54.1:33-41,2019
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130.Progress in characterizing the linkage between Fusobacterium
nucleatum and gastrointestinal cancer
Liu, Yang; Baba, Yoshifumi; Ishimoto, Takatsugu; Iwatsuki, Masaaki;
Hiyoshi, Yukiharu; Miyamoto, Yuji; Yoshida, Naoya; Wu, Rong;Baba, Hideo
JOURNAL OF GASTROENTEROLOGY 54.1:33-41,2019
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131.Defining compartmentalized stem cell populations with
distinct cell division dynamics in the ocular surface
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Ishii, Ryutaro; Yanagisawa, Hiromi; Sada, Aiko
DEVELOPMENT 147.24:n/a,2020 n/a Article
132.Four-dimensional imaging with virtual reality to quantitatively
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cotyledon pavement cells
Higaki, Takumi; Mizuno, Hidenobu
PLANT BIOTECHNOLOGY 37.4:429-435,2020
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133.Blood and lymphatic systems are segregated by the FLCN
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Tai-Nagara, Ikue; Hasumi, Yukiko; Kusumoto, Dai; Hasumi, Hisashi;
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Saya, Hideyuki; Liu, Chang; Li, Wenling; Mukouyama, Yoh-suke;
Marston Linehan, W.; Liu, Xinyi; Hirashima, Masanori; Suzuki,
Yutaka; Funasaki, Shintaro; Satou, Yorifumi; Furuya, Mitsuko; Baba,
Masaya; Kubota, Yoshiaki
NATURE COMMUNICATIONS 11.1:n/a,2020 MEDLINE:33298956
Article134.Machine Learning of Hematopoietic Stem Cell Divisions from
Paired Daughter Cell Expression Profiles Reveals Effects ofAging on Self-Renewal
Arai, Fumio; Stumpf, Patrick S.; Ikushima, Yoshiko M.; Hosokawa,
Kentaro; Roch, Aline; Lutolf, Matthias P.; Suda, Toshio; MacArthur,
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135.Fusobacterium nucleatum confers chemoresistance bymodulating autophagy in oesophageal squamous cell
carcinoma
Liu, Yang; Baba, Yoshifumi; Ishimoto, Takatsugu; Tsutsuki, Hiroyasu;
Zhang, Tianli; Nomoto, Daichi; Okadome, Kazuo; Yamamura,Kensuke; Harada, Kazuto; Eto, Kojiro; Hiyoshi, Yukiharu; Iwatsuki,
Masaaki; Nagai, Yohei; Iwagami, Shiro; Miyamoto, Yuji; Yoshida,
Naoya; Komohara, Yoshihiro; Ohmuraya, Masaki; Wang, Xiaoming;
Ajani, Jaffer A.; Sawa, Tomohiro; Baba, Hideo
BRITISH JOURNAL OF CANCER 124.5:963-974,2020
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136.Immuno-Modulation of Hematopoietic Stem and Progenitor
Cells in Inflammation
Sezaki, Maiko; Hayashi, Yoshikazu; Wang, Yuxin; Johansson,
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FRONTIERS IN IMMUNOLOGY 11.n/a:n/a,2020
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137.A new perfusion culture method with a self-organizedcapillary network
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PLOS ONE 15.10:n/a,2020 MEDLINE:33112918 Article
138.Trastuzumab upregulates programmed death ligand-1
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Yamashita, Kohei; Iwatsuki, Masaaki; Yasuda-Yoshihara, Noriko;Morinaga, Takeshi; Nakao, Yosuke; Harada, Kazuto; Eto, Kojiro;
Kurashige, Junji; Hiyoshi, Yukiharu; Ishimoto, Takatsugu; Nagai,
Yohei; Iwagami, Shiro; Baba, Yoshifumi; Miyamoto, Yuji; Yoshida,
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BRITISH JOURNAL OF CANCER 124.3:595-603,2020
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139.Hematopoietic stem cells acquire survival advantage by loss
of RUNX1 methylation identified in familial leukemia
Matsumura, Takayoshi; Nakamura-Ishizu, Ayako; Muddineni, Siva
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Tokunaga, Kenji; Tirado-Magallanes, Roberto; Sian, Stephanie;
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Osato, Motomi; Suda, Toshio
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140.Developmental Phase Transitions in Spatial Organization of
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Nakazawa, Shingo; Yoshimura, Yumiko; Takagi, Masahiro; Mizuno,
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JOURNAL OF NEUROSCIENCE 40.40:7637-7650,2020
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141.Extracellular Vesicles from Cancer-Associated Fibroblasts
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Stabilizing ﾀ1 Integrin, Enhancing Drug ResistanceUchihara, Tomoyuki; Miyake, Keisuke; Yonemura, Atsuko;
Komohara, Yoshihiro; Itoyama, Rumi; Koiwa, Mayu; Yasuda,
Tadahito; Arima, Kota; Harada, Kazuto; Eto, Kojiro; Hayashi,
Hiromitsu; Iwatsuki, Masaaki; Iwagami, Shiro; Baba, Yoshifumi;
Yoshida, Naoya; Yashiro, Masakazu; Masuda, Mari; Ajani, Jaffer A.;
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CANCER RESEARCH 80.16:3222-3235,2020 MEDLINE:32605995
Article142.PRC2 insufficiency causes p53-dependent dyserythropoiesis
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143.Functional diversity of cancer-associated fibroblasts inmodulating drug resistance
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Ishimoto, Takatsugu
CANCER SCIENCE 111.10:3468-3477,2020 MEDLINE:33044028
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144.EVI1 triggers metabolic reprogramming associated withleukemogenesis and increases sensitivity to L-asparaginase
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Kamimura, Sachiyo; Suekane, Akira; Ogoh, Honami; Matsuo,
Hidemasa; Adachi, Souichi; Taga, Takashi; Tomizawa, Daisuke;
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Ramilowski, Jordan A.; Abugessaisa, Imad; Ishizu, Yuri; Noma,
Shohei; Tarui, Hiroshi; Taylor, Martin S.; Lassmann, Timo; Itoh,
Masayoshi; Kasukawa, Takeya; Kawaji, Hideya; Marchionni,
Luigi; Sheng, Guojun; Forrest, Alistair R. R.; Khachigian, Levon M.;
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147.Establishment of bone marrow-derived M-CSF receptordependent self-renewing macrophages
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CELL DEATH DISCOVERY 6.1:n/a,2020 n/a Article
148.Calreticulin haploinsufficiency augments stem cell activity
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Nakamura, Kenichi; Hidaka, Tomonori; Kubuki, Yoko; Iwakiri,
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149.Glycome profiling by lectin microarray reveals dynamic glycan
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150.Tumor immune microenvironment and immune checkpoint
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Baba, Yoshifumi; Nomoto, Daichi; Okadome, Kazuo; Ishimoto,Takatsugu; Iwatsuki, Masaaki; Miyamoto, Yuji; Yoshida, Naoya;
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151.Diphtheria toxin-mediated transposon-driven poly (A)-trapping
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Bai, Jie; Kondo, Ryohei; Mayasari, N. Ika; Shigeoka, Toshiaki;
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Ishida, Yasumasa
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152.Prognostic Nutritional Index, Tumor-infiltrating Lymphocytes,
and Prognosis in Patients with Esophageal Cancer
Okadome, Kazuo; Baba, Yoshifumi; Yagi, Taisuke; Kiyozumi, Yuki;
Ishimoto, Takatsugu; Iwatsuki, Masaaki; Miyamoto, Yuji; Yoshida,
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ANNALS OF SURGERY 271.4:693-700,2020 MEDLINE:30308614
Article153.Overexpression of RUNX3 Represses RUNX1 to Drive
Transformation of Myelodysplastic Syndrome
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Akinori; Tanaka, Daiki; Oshima, Motohiko; Harada, Yuka; Ohyashiki,
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CANCER RESEARCH 80.12:2523-2536,2020 MEDLINE:32341038
Article154.Developmental origins of health and disease theory in
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Arima, Yuichiro; Fukuoka, Hideoki
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155.Guidelines and definitions for research on epithelialmesenchymal transition
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Rik; Ford, Heide L.; Fuxe, Jonas; Garcia de Herreros, Antonio;
Goodall, Gregory J.; Hadjantonakis, Anna-Katerina; Huang, Ruby
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Yeesim; Levine, Herbert; Liu, Jinsong; Longmore, Gregory D.;
Mani, Sendurai A.; Massague, Joan; Mayor, Roberto; McClay,David; Mostov, Keith E.; Newgreen, Donald F.; Nieto, M. Angela;
Puisieux, Alain; Runyan, Raymond; Savagner, Pierre; Stanger,
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NATURE REVIEWS MOLECULAR CELL BIOLOGY 21.6:341352,2020 MEDLINE:32300252 Review
156.RhoJ integrates attractive and repulsive cues in directionalmigration of endothelial cells
Fukushima, Yoko; Nishiyama, Koichi; Kataoka, Hiroshi; Fruttiger,
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157.Clinical Importance of Mean Corpuscular Volume as a
Prognostic Marker After Esophagectomy for Esophageal
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Yoshida, Naoya; Kosumi, Keisuke; Tokunaga, Ryuma; Yoshifumi,
Baba; Nagai, Yohei; Miyamoto, Yuji; Iwagami, Shiro; Iwatsuki,
Masaaki; Hiyoshi, Yukiharu; Ishimoto, Takatsugu; Eto, Kojiro;
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Article158.FLCN alteration drives metabolic reprogramming towards
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Kawahara, Takashi; Ito, Yusuke; Muraoka, Kentaro; Nakaigawa,
Noboru; Kimura, Yayoi; Baba, Masaya; Nagahama, Kiyotaka;
Takahata, Hiroyuki; Saito, Ichiro; Schmidt, Laura S.; Linehan, W.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCHCOMMUNICATIONS 522.4:931-938,2020 MEDLINE:31806376
Article159.Mesenchymal-epithelial transition regulates initiation ofpluripotency exit before gastrulation
Hamidi, Sofiane; Nakaya, Yukiko; Nagai, Hiroki; Alev, Cantas;
Kasukawa, Takeya; Chhabra, Sapna; Lee, Ruda; Niwa, Hitoshi;
Warmflash, Aryeh; Shibata, Tatsuo; Sheng, Guojun
DEVELOPMENT 147.3:n/a,2020 MEDLINE:32014865 Article
160.RUNX3 levels in human hematopoietic progenitors are
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Balogh, Peter; Adelman, Emmalee R.; Pluvinage, John, V; Capaldo,
Brian J.; Freeman, Katie C.; Singh, Sandeep; Elagib, Kamaleldin
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Li, Hui; Gru, Alejandro A.; Price, Elizabeth A.; Schrier, Stanley L.;
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161.Systematic Review of Normal Subjects Harbouring BCR-ABL1
Fusion Gene
Kuan, Jew Win; Su, Anselm Ting; Leong, Chooi Fun; Osato,
Motomi; Sashida, Goro
ACTA HAEMATOLOGICA 143.2:96-111,2020 MEDLINE:31401626
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162.Prognostic and clinical impact of PD-L2 and PD-L1 expression
in a cohort of 437 oesophageal cancers
Okadome, Kazuo; Baba, Yoshifumi; Nomoto, Daichi; Yagi, Taisuke;
Kalikawe, Rebecca; Harada, Kazuto; Hiyoshi, Yukiharu; Nagai,
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Miyamoto, Yuji; Yoshida, Naoya; Watanabe, Masayuki; Komohara,
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BRITISH JOURNAL OF CANCER 122.10:1535-1543,2020
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163.iPSC-Derived Platelets Depleted of HLA Class I Are Inert toAnti-HLA Class I and Natural Killer Cell Immunity
Suzuki, Daisuke; Flahou, Charlotte; Yoshikawa, Norihide; Stirblyte,
Ieva; Hayashi, Yoshikazu; Sawaguchi, Akira; Akasaka, Marina;
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Takuya; Fujio, Kosuke; Manz, Markus G.; Hotta, Akitsu; Takizawa,
Hitoshi; Eto, Koji; Sugimoto, Naoshi
STEM CELL REPORTS 14.1:49-59,2020 MEDLINE:31883921
Article164.Histone H3 K4/9/27 Trimethylation Levels Affect Wound
Healing and Stem Cell Dynamics in Adult Skin
Kang, Sangjo; Long, Kylie; Wang, Sherry; Sada, Aiko; Tumbar,
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Article165.A FLCN- TFE3 Feedback Loop Prevents Excessive
Glycogenesis and Phagocyte Activation by Regulating
Lysosome Activity
Endoh, Mitsuhiro; Baba, Masaya; Endoh, Tamie; Hirayama,
Akiyoshi; Nakamura-Ishizu, Ayako; Umemoto, Terumasa;
Hashimoto, Michihiro; Nagashima, Kunio; Soga, Tomoyoshi; Lang,
Martin; Schmidt, Laura S.; Linehan, W. Marston; Suda, Toshio
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166.Gastric Cancer Stem Cells: Current Insights into the Immune
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Fu, Lingfeng; Bu, Luke; Yasuda, Tadahito; Koiwa, Mayu; Akiyama,
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BIOMEDICINES 8.1:n/a,2020 MEDLINE:31935894 Review
167.Prognostic impacts of the combined positive score andthe tumor proportion score for programmed death ligand-1
expression by double immunohistochemical staining inpatients with advanced gastric cancer
Yamashita, Kohei; Iwatsuki, Masaaki; Harada, Kazuto; Eto, Kojiro;
Hiyoshi, Yukiharu; Ishimoto, Takatsugu; Nagai, Yohei; Iwagami,
Shiro; Miyamoto, Yuji; Yoshida, Naoya; Komohara, Yoshihiro; Ajani,
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GASTRIC CANCER 23.1:95-104,2020 MEDLINE:31451991 Article
168.A two-pronged anti-leukemic agent based on a hyaluronic
acid-green tea catechin conjugate for inducing targeted cell
death and terminal differentiation
Liang, Kun; Bae, Ki Hyun; Nambu, Akiko; Dutta, Bibek; Chung, Joo
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BIOMATERIALS SCIENCE 8.1:497-505,2020 MEDLINE:31761907
Article169.Elongation of the high right atrium to coronary sinus
conduction time predicts the recurrence of atrial fibrillation
after radiofrequency catheter ablation
Kanemaru, Yusuke; Arima, Yuichiro; Kaikita, Koichi; Kiyama,
Takuya; Kaneko, Shozo; Ito, Miwa; Yamabe, Hiroshige; Motozato,
Kota; Yamanaga, Kenshi; Fujisue, Koichiro; Sueta, Daisuke;
Takashio, Seiji; Araki, Satoshi; Usuku, Hiroki; Nakamura, Taishi;
Fukunaga, Takashi; Suzuki, Satoru; Izumiya, Yasuhiro; Sakamoto,
Kenji; Soejima, Hirofumi; Yamamoto, Eiichiro; Kawano, Hiroaki;
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INTERNATIONAL JOURNAL OF CARDIOLOGY 300.n/a:147153,2020 MEDLINE:31785957 Article
170.Cardiomyocyte Sirt (Sirtuin) 7 Ameliorates Stress-Induced
Cardiac Hypertrophy by Interacting With and Deacetylating
GATA4
Yamamura, Satoru; Izumiya, Yasuhiro; Araki, Satoshi; Nakamura,
Taishi; Kimura, Yuichi; Hanatani, Shinsuke; Yamada, Toshihiro;
Ishida, Toshifumi; Yamamoto, Masahiro; Onoue, Yoshiro; Arima,
Yuichiro; Yamamoto, Eiichiro; Sunagawa, Yoichi; Yoshizawa,
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Tsujita, Kenichi
HYPERTENSION 75.1:98-108,2020 MEDLINE:31735083 Article
171.Vascularized cancer on a chip: The effect of perfusion on
growth and drug delivery of tumor spheroid
Nashimoto, Yuji; Okada, Ryu; Hanada, Sanshiro; Arima, Yuichiro;
Nishiyama, Koichi; Miura, Takashi; Yokokawa, Ryuji
BIOMATERIALS 229.n/a:n/a,2020 MEDLINE:31710953 Article
172.A novel PDK1 inhibitor, JX06, inhibits glycolysis and induces
apoptosis in multiple myeloma cells
Kawano, Yawara; Sasano, Takayuki; Arima, Yuichiro; Kushima,
Saki; Tsujita, Kenichi; Matsuoka, Masao; Hata, Hiroyuki
BIOCHEMICAL AND BIOPHYSICAL RESEARCHCOMMUNICATIONS 587.n/a:153-159,2021 MEDLINE:34875534
Article173.The primitive streak and cellular principles of building anamniote body through gastrulation
Sheng, Guojun; Arias, Alfonso Martinez; Sutherland, Ann
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174.PD-L1 and PD-L2 expression s ta tus in rela tion tochemotherapy in primary and metastatic esophageal
squamous cell carcinoma
Okadome, Kazuo; Baba, Yoshifumi; Yasuda-Yoshihara, Noriko;Nomoto, Daichi; Yagi, Taisuke; Toihata, Tasuku; Ogawa, Katsuhiro;
Sawayama, Hiroshi; Ishimoto, Takatsugu; Iwatsuki, Masaaki;
Iwagami, Shiro; Miyamoto, Yuji; Yoshida, Naoya; Watanabe,
Masayuki; Komohara, Yoshihiro; Baba, Hideo
CANCER SCIENCE 113.2:399-410,2021 MEDLINE:34773342
Article175.Sirt7 Deficiency Attenuates Neointimal Formation Following
Vascular Injury by Modulating Vascular Smooth Muscle Cell
Proliferation
Kimura, Yuichi; Izumiya, Yasuhiro; Araki, Satoshi; Yamamura,
Satoru; Hanatani, Shinsuke; Onoue, Yoshiro; Ishida, Toshifumi;
Arima, Yuichiro; Nakamura, Taishi; Yamamoto, Eiichiro; Senokuchi,
Takafumi; Yoshizawa, Tatsuya; Sata, Masataka; Kim-Mitsuyama,
Shokei; Nakagata, Naomi; Bober, Eva; Braun, Thomas; Kaikita,
Koichi; Yamagata, Kazuya; Tsujita, Kenichi
CIRCULATION JOURNAL 85.12:2232-2240,2021
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176.Establishment of an immortalized human erythroid cell line
sustaining differentiation potential without inducible gene
expression system
Soboleva, Svetlana; Kurita, Ryo; Kajitani, Naoko; Akerstrand, Hugo;
Miharada, Kenichi
HUMAN CELL 35.1:408-417,2021 MEDLINE:34817797 Article
177.Cellular senescence in the tumor microenvironment andcontext-specific cancer treatment strategies
Yasuda, Tadahito; Baba, Hideo; Ishimoto, Takatsugu
FEBS JOURNAL 290.5:1290-1302,2021 MEDLINE:34653317
Review
178.Metabolic shift to serine biosynthesis through 3-PG
accumulation and PHGDH induction promotes tumor growthin pancreatic cancer
Itoyama, Rumi; Yasuda-Yoshihara, Noriko; Kitamura, Fumimasa;
Yasuda, Tadahito; Bu, Luke; Yonemura, Atsuko; Uchihara,
Tomoyuki; Arima, Kota; Hu, Xichen; Jun, Zhang; Okamoto, Yuya;
Akiyama, Takahiko; Yamashita, Kohei; Nakao, Yosuke; Yusa,
Toshihiko; Kitano, Yuki; Higashi, Takaaki; Miyata, Tatsunori; Imai,
Katsunori; Hayashi, Hiromitsu; Yamashita, Yo-ichi; Mikawa, Takumi;
Kondoh, Hiroshi; Baba, Hideo; Ishimoto, Takatsugu
CANCER LETTERS 523.n/a:29-42,2021 MEDLINE:34508795
Article179.Isolation and Culture of Primary Oral Keratinocytes from the
Adult Mouse Palate
Ngo, Yen Xuan; Haga, Kenta; Suzuki, Ayako; Kato, Hiroko;
Yanagisawa, Hiromi; Izumi, Kenji; Sada, Aiko
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS n/a.175:n/a,2021 MEDLINE:34633387 Article
180.Relationship between Fusobacterium nucleatum andantitumor immunity in colorectal cancer liver metastasis
Sakamoto, Yuki; Mima, Kosuke; Ishimoto, Takatsugu; Ogata,
Yoko; Imai, Katsunori; Miyamoto, Yuji; Akiyama, Takahiko; Daitoku,
Nobuya; Hiyoshi, Yukiharu; Iwatsuki, Masaaki; Baba, Yoshifumi;
Iwagami, Shiro; Yamashita, Yo-ichi; Yoshida, Naoya; Komohara,
Yoshihiro; Ogino, Shuji; Baba, Hideo
CANCER SCIENCE 112.11:4470-4477,2021 MEDLINE:34464993
Article181.Irf5 siRNA-loaded biodegradable lipid nanoparticles
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Kawase, Wataru; Kurotaki, Daisuke; Suzuki, Yuta; Ishihara,
Hiroshi; Ban, Tatsuma; Sato, Go R.; Ichikawa, Juri; Yanai, Hideyuki;
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MOLECULAR THERAPY-NUCLEIC ACIDS 25.n/a:708-715,2021
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182.Insufficiency of non-canonical PRC1 synergizes with
JAK2V617F in the development of myelofibrosis
Shinoda, Daisuke; Nakajima-Takagi, Yaeko; Oshima, Motohiko;
Koide, Shuhei; Aoyama, Kazumasa; Saraya, Atsunori; Harada,
Hironori; Rahmutulla, Bahityar; Kaneda, Atsushi; Yamaguchi,
Kiyoshi; Furukawa, Yoichi; Koseki, Haruhiko; Shimoda, Kazuya;
Tanaka, Tomoaki; Sashida, Goro; Iwama, Atsushi
LEUKEMIA 36.2:452-463,2021 MEDLINE:34497325 Article
183.Assessment of the Diagnostic Efficiency of a Liquid Biopsy
Assay for Early Detection of Gastric Cancer
Izumi, Daisuke; Zhu, Zhongxu; Chen, Yuetong; Toden, Shusuke;
Huo, Xinying; Kanda, Mitsuro; Ishimoto, Takatsugu; Gu, Dongying;Tan, Miaomiao; Kodera, Yasuhiro; Baba, Hideo; Li, Wei; Chen,
Jinfei; Wang, Xin; Goel, Ajay
JAMA NETWORK OPEN 4.8:n/a,2021 MEDLINE:34427680 Article

184.Remote control of neural function by X-ray-induced
scintillation
Matsubara, Takanori; Yanagida, Takayuki; Kawaguchi, Noriaki;
Nakano, Takashi; Yoshimoto, Junichiro; Sezaki, Maiko; Takizawa,
Hitoshi; Tsunoda, Satoshi P.; Horigane, Shin-ichiro; Ueda, Shuhei;
Takemoto-Kimura, Sayaka; Kandori, Hideki; Yamanaka, Akihiro;
Yamashita, Takayuki
NATURE COMMUNICATIONS 12.1:n/a,2021 MEDLINE:34294698
Article185.Thrombopoietin maintains cell numbers of hematopoietic
stem and progenitor cells with megakaryopoietic potential
O’Neill, Aled; Chin, Desmond; Tan, Darren; Majeed, A’Qilah Banu
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HAEMATOLOGICA 106.7:1883-1891,2021 MEDLINE:32527954
Article186.East Asian variant aldehyde dehydrogenase type 2 genotype
exacerbates ischemia/reperfusion injury with ST-elevation
myocardial infarction in men: possible sex differences
Ishida, Toshifumi; Arima, Yuichiro; Mizuno, Yuji; Harada, Eisaku;
Yamashita, Takayoshi; Sueta, Daisuke; Sakamoto, Kenji; Suzuki,
Satoru; Kaikita, Koichi; Yamada, Yoshihiro; Shimomura, Hideki;
Oniki, Kentaro; Saruwatari, Junji; Hokimoto, Seiji; Yasue, Hirofumi;
Tsujita, Kenichi
HEART AND VESSELS 37.2:184-193,2021 MEDLINE:34259924
Article187.Prognostic significance of liver stiffness assessed by
fibrosis-4 index in patients with heart failure
Takae, Masafumi; Fujisue, Koichiro; Yamamoto, Eiichiro; Egashira,
Koichi; Komorita, Takashi; Oike, Fumi; Nishihara, Taiki; Yamamoto,
Masahiro; Hirakawa, Kyoko; Tabata, Noriaki; Tokitsu, Takanori;
Yamanaga, Kenshi; Sueta, Daisuke; Hanatani, Shinsuke;
Nakamura, Taishi; Usuku, Hiroki; Araki, Satoshi; Arima, Yuichiro;
Takashio, Seiji; Suzuki, Satoru; Kaikita, Koichi; Matsushita, Kenichi;
Tsujita, Kenichi
ESC HEART FAILURE 8.5:3809-3821,2021 MEDLINE:34156170
Article188.Identification of potential chemical compounds enhancing
generation of enucleated cells from immortalized humanerythroid cell lines
Soboleva, Svetlana; Kurita, Ryo; Ek, Fredrik; Akerstrand, Hugo;
Silverio-Alves, Rita; Olsson, Roger; Nakamura, Yukio; Miharada,
Kenichi
COMMUNICATIONS BIOLOGY 4.1:n/a,2021 MEDLINE:34083702
Article189.Inflammation-Induced Tumorigenesis and Metastasis
Hibino, Sana; Kawazoe, Tetsuro; Kasahara, Hidenori; Itoh, Shinji;
Ishimoto, Takatsugu; Sakata-Yanagimoto, Mamiko; Taniguchi, Koji
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
22.11:n/a,2021 MEDLINE:34063828 Review
190.Elucidating mechanisms of neuronal circuit formation in layer
4 of the somatosensory cortex via intravital imaging
Rao, Madhura S.; Mizuno, Hidenobu
NEUROSCIENCE RESEARCH 167.n/a:47-53,2021
MEDLINE:33309867 Review
191.CD271(+)CD51(+)PALLADIN(-) Human Mesenchymal Stromal
Cells Possess Enhanced Ossicle-Forming Potential
Sezaki, Maiko; Biswas, Subinoy; Nakata, Sayuri; Oshima, Motohiko;
Koide, Shuhei; Ho, Nicole Pui Yu; Okamoto, Nobukazu; Miyamoto,Takeshi; Iwama, Atsushi; Takizawa, Hitoshi
STEM CELLS AND DEVELOPMENT 30.14:725-735,2021
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192.Super-enhancers for RUNX3 are required for cell proliferationin EBV-infected B cell lines
Hosoi, Hiroki; Niibori-Nambu, Akiko; Nah, Giselle Sek Suan;
Bahirvani, Avinash Govind; Mok, Michelle Meng Huang; Sanda,
Takaomi; Kumar, Alan Prem; Tenen, Daniel G.; Ito, Yoshiaki; Sonoki,Takashi; Osato, Motomi
GENE 774.n/a:n/a,2021 MEDLINE:33444684 Article
193.Mitochondria transfer from early stages of erythroblasts totheir macrophage niche via tunnelling nanotubes
Yang, Chong; Endoh, Mitsuhiro; Tan, Darren Q.; Nakamura-Ishizu,
Ayako; Takihara, Yuji; Matsumura, Takayoshi; Suda, Toshio
BRITISH JOURNAL OF HAEMATOLOGY 193.6:1260-1274,2021
MEDLINE:34036571 Article
194.Defining epithelial-mesenchymal transitions in animal
development
Sheng, Guojun
DEVELOPMENT 148.8:n/a,2021 MEDLINE:33913481 Article
195.Prolonged maintenance of hematopoietic stem cells thatescape from thrombopoietin deprivation
Nakamura-Ishizu, Ayako; Chin, Desmond Wai Loon; Matsumura,Takayoshi; Tan, Darren Qiancheng; Mochizuki-Kashio, Makiko;Deng Jianwen; Suda, Toshio
BLOOD 137.19:2609-2620,2021 MEDLINE:33657206 Article
196.Dysregulation of Amphiregulin stimulates the pathogenesis of
cystic lymphangioma
Yoshida, Naofumi; Yamamoto, Seiji; Hamashima, Takeru; Okuno,
Noriko; Okita, Naruho; Horikawa, Shinjiro; Hayashi, Masao; Thanh
Chung Dang; Quang Linh Nguyen; Nishiyama, Koichi; Makino,
Teruhiko; Ishii, Yoko; Tomihara, Kei; Shimizu, Tadamichi; Shibuya,
Masabumi; Noguchi, Makoto; Sasahara, Masakiyo
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES
OF THE UNITED STATES OF AMERICA 118.19:n/a,2021
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197.Mitochondria Turnover and Lysosomal Function in
Hematopoietic Stem Cell Metabolism
Mochizuki-Kashio, Makiko; Shiozaki, Hiroko; Suda, Toshio;
Nakamura-Ishizu, Ayako
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
22.9:n/a,2021 MEDLINE:33924874 Review
198.Two faces of RUNX3 in myeloid transformation
Yokomizo-Nakano, Takako; Sashida, Goro
EXPERIMENTAL HEMATOLOGY 97.n/a:14-20,2021
MEDLINE:33600870 Review
199.UTX maintains the functional integrity of the murine
hematopoietic system by globally regulating aging-associatedgenes
Sera, Yasuyuki; Nakata, Yuichiro; Ueda, Takeshi; Yamasaki,
Norimasa; Koide, Shuhei; Kobayashi, Hiroshi; Ikeda, Ken-ichiro;
Kobatake, Kohei; Iwasaki, Masayuki; Oda, Hideaki; Wolff, Linda;
Kanai, Akinori; Nagamachi, Akiko; Inaba, Toshiya; Sotomaru,
Yusuke; Ichinohe, Tatsuo; Koizumi, Miho; Miyakawa, Yoshihiko;
Honda, Zen-ichiro; Iwama, Atsushi; Suda, Toshio; Takubo, Keiyo;
Honda, Hiroaki
BLOOD 137.7:908-922,2021 MEDLINE:33174606 Article
200.Contribution of PDGFR alpha-positive cells in maintenanceand injury responses in mouse large vessels
Kimura, Kenichi; Ramirez, Karina; Nguyen, Tram Anh Vu;
Yamashiro, Yoshito; Sada, Aiko; Yanagisawa, Hiromi
SCIENTIFIC REPORTS 11.1:n/a,2021 MEDLINE:33883668 Article

201.Semaphorin3E-PlexinD1 signaling in coronary artery andlymphatic vessel development with clinical implications inmyocardial recovery
Maruyama, Kazuaki; Naemura, Kazuaki; Arima, Yuichiro; Uchijima,
Yasunobu; Nagao, Hiroaki; Yoshihara, Kenji; Singh, Manvendra K.;
Uemura, Akiyoshi; Matsuzaki, Fumio; Yoshida, Yutaka; Kurihara,
Yukiko; Miyagawa-Tomita, Sachiko; Kurihara, Hiroki
ISCIENCE 24.4:n/a,2021 MEDLINE:33870127 Article
202.Fit-For-All iPSC-Derived Cell Therapies and Their Evaluation in
Humanized Mice With NK Cell Immunity
Flahou, Charlotte; Morishima, Tatsuya; Takizawa, Hitoshi; Sugimoto,
NaoshiFRONTIERS IN IMMUNOLOGY 12.n/a:n/a,2021
MEDLINE:33897711 Review
203.Autophagy is dispensable for the maintenance ofhematopoietic stem cells in neonates
Hashimoto, Michihiro; Umemoto, Terumasa; Nakamura-Ishizu,
Ayako; Matsumura, Takayoshi; Yokomizo, Tomomasa; Sezaki,
Maiko; Takizawa, Hitoshi; Suda, Toshio
BLOOD ADVANCES 5.6:1594-1604,2021 MEDLINE:33710340
Article204.Epithelial-Mesenchymal Transition in Liver Fluke-Induced
Cholangiocarcinoma
Sawanyawisuth, Kanlayanee; Sashida, Goro; Sheng, Guojun
CANCERS 13.4:n/a,2021 MEDLINE:33672838 Review
205.Murine neonatal ketogenesis preserves mitochondrialenergetics by preventing protein hyperacetylation
Arima, Yuichiro; Nakagawa, Yoshiko; Takeo, Toru; Ishida, Toshifumi;
Yamada, Toshihiro; Hino, Shinjiro; Nakao, Mitsuyoshi; Hanada,
Sanshiro; Umemoto, Terumasa; Suda, Toshio; Sakuma, Tetsushi;
Yamamoto, Takashi; Watanabe, Takehisa; Nagaoka, Katsuya;
Tanaka, Yasuhito; Kawamura, Yumiko K.; Tonami, Kazuo; Kurihara,
Hiroki; Sato, Yoshifumi; Yamagata, Kazuya; Nakamura, Taishi; Araki,
Satoshi; Yamamoto, Eiichiro; Izumiya, Yasuhiro; Sakamoto, Kenji;
Kaikita, Koichi; Matsushita, Kenichi; Nishiyama, Koichi; Nakagata,
Naomi; Tsujita, Kenichi
NATURE METABOLISM 3.2:196-+,2021 MEDLINE:33619377
Article206.Inflammation-driven senescence-associated secretory
phenotype in cancer-associated fibroblasts enhances
peritoneal dissemination
Yasuda, Tadahito; Koiwa, Mayu; Yonemura, Atsuko; Miyake,
Keisuke; Kariya, Ryusho; Kubota, Sho; Yokomizo-Nakano, Takako;
Yasuda-Yoshihara, Noriko; Uchihara, Tomoyuki; Itoyama, Rumi; Bu,
Luke; Fu, Lingfeng; Arima, Kota; Izumi, Daisuke; Iwagami, Shiro;
Eto, Kojiro; Iwatsuki, Masaaki; Baba, Yoshifumi; Yoshida, Naoya;
Ohguchi, Hiroto; Okada, Seiji; Matsusaki, Keisuke; Sashida, Goro;
Takahashi, Akiko; Tan, Patrick; Baba, Hideo; Ishimoto, Takatsugu
CELL REPORTS 34.8:n/a,2021 MEDLINE:33626356 Article
207.Epithelial-Mesenchymal Transition Drives Three-Dimensional
Morphogenesis in Mammalian Early Development
Ismagulov, Galym; Hamidi, Sofiane; Sheng, Guojun
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 9.n/a:n/a,2021 MEDLINE:33644076 Article
208.NMDA Receptor Enhances Correlation of Spontaneous
Activity in Neonatal Barrel Cortex
Mizuno, Hidenobu; Rao, Madhura S.; Mizuno, Hiromi; Sato, Takuya;
Nakazawa, Shingo; Iwasato, Takuji
JOURNAL OF NEUROSCIENCE 41.6:1207-1217,2021
MEDLINE:33372060 Article― 68 ―

209.Vasculature-driven stem cell population coordinates tissue
scaling in dynamic organs
Ichijo, R.; Kabata, M.; Kidoya, H.; Muramatsu, F.; Ishibashi, R.; Abe,K.; Tsutsui, K.; Kubo, H.; Iizuka, Y.; Kitano, S.; Miyachi, H.; Kubota, Y.;
Fujiwara, H.; Sada, A.; Yamamoto, T.; Toyoshima, F.
SCIENCE ADVANCES 7.7:n/a,2021 MEDLINE:33568475 Article
210.Overexpression of Hmga2 activates Igf2bp2 and remodels
transcriptional program of Tet2-deficient stem cells in myeloid
transformation
Bai, Jie; Yokomizo-Nakano, Takako; Kubota, Sho; Sun, Yuqi; Kanai,
Akinori; Iimori, Mihoko; Harada, Hironori; Iwama, Atsushi; Sashida,
Goro
ONCOGENE 40.8:1531-1541,2021 MEDLINE:33452460 Article
211.Myeloma cells self-promote migration by regulating TAB1driven TIMP-1 expression in mesenchymal stem cells
Rethnam, Malini; Tan, Darren Qiancheng; Suda, Toshio
BIOCHEMICAL AND BIOPHYSICAL RESEARCHCOMMUNICATIONS 534.n/a:843-848,2021 MEDLINE:33183761
Article212.Single-cell transcriptomes underscore genetically distinct
tumor characteristics and microenvironment for hereditarykidney cancers
Jikuya, Ryosuke; Murakami, Koichi; Nishiyama, Akira; Kato,
Ikuma; Furuya, Mitsuko; Nakabayashi, Jun; Ramilowski, Jordan A.;
Hamanoue, Haruka; Maejima, Kazuhiro; Fujita, Masashi; Mitome,
Taku; Ohtake, Shinji; Noguchi, Go; Kawaura, Sachi; Odaka,
Hisakazu; Kawahara, Takashi; Komeya, Mitsuru; Shinoki, Risa;
Ueno, Daiki; Ito, Hiroki; Ito, Yusuke; Muraoka, Kentaro; Hayashi,
Narihiko; Kondo, Keiichi; Nakaigawa, Noboru; Hatano, Koji;
Baba, Masaya; Suda, Toshio; Kodama, Tatsuhiko; Fujii, Satoshi;
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Tomohiko; Hasumi, Hisashi
ISCIENCE 25.6:n/a,2022 MEDLINE:35874919 Article
213.Highly efficient Runx1 enhancer eR1-mediated genetic
engineering for fetal, child and adult hematopoietic stem cells
Koh, Cai Ping; Bahirvani, Avinash Govind; Wang, Chelsia Qiuxia;
Yokomizo, Tomomasa; Ng, Cherry Ee Lin; Du, Linsen; Tergaonkar,
Vinay; Voon, Dominic Chih-Cheng; Kitamura, Hiroaki; Hosoi, Hiroki;
Sonoki, Takashi; Michelle, Mok Meng Huang; Tan, Lii Jye; NiiboriNambu, Akiko; Zhang, Yi; Perkins, Archibald S.; Hossain, Zakir;
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GENE 851.n/a:n/a,2022 MEDLINE:36384171 Article
214.Quantitative Analysis of Sympathetic and Nociceptive
Innervation Across Bone Marrow Regions in Mice
Fujita, Shinya; Morikawa, Takayuki; Tamaki, Shinpei; Sezaki, Maiko;
Takizawa, Hitoshi; Okamoto, Shinichiro; Kataoka, Keisuke; Takubo,
Keiyo
EXPERIMENTAL HEMATOLOGY 112.n/a:44-+,2022
MEDLINE:35907584 Article
215.Hepatic leukemia factor-expressing paraxial mesoderm cells
contribute to the developing brain vasculature
Koui, Yuta; Ideue, Takako; Boylan, Michael; Anderson, Matthew J.;
Osato, Motomi; Suda, Toshio; Yokomizo, Tomomasa; Mukouyama,
Yoh-suke
BIOLOGY OPEN 11.9:n/a,2022 MEDLINE:36017733 Article
216.Pericytes and shear stress each alter the shape of a selfassembled vascular network
Fujimoto, Kazuya; Erickson, Scott; Nakayama, Masamune; Ihara,
Hiroki; Sugihara, Kei; Nashimoto, Yuji; Nishiyama, Koichi; Miura,
Takashi; Yokokawa, Ryuji
LAB ON A CHIP 23.2:306-317,2022 MEDLINE:36537555 Article

217.IL-1 beta derived from mixed-polarized macrophages activates
fibroblasts and synergistically forms a cancer-promoting
microenvironment
Zhang, Jun; Fu, Lingfeng; Yasuda-Yoshihara, Noriko; Yonemura,
Atsuko; Wei, Feng; Bu, Luke; Hu, Xichen; Akiyama, Takahiko;
Kitamura, Fumimasa; Yasuda, Tadahito; Semba, Takashi; Uchihara,
Tomoyuki; Itoyama, Rumi; Yamashita, Kohei; Eto, Kojiro; Iwagami,
Shiro; Yashiro, Masakazu; Komohara, Yoshihiro; Baba, Hideo;
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218.Tsukushi proteoglycan maintains RNA splicing anddevelopmental signaling network in GFAP-expressing
subventricular zone neural stem/progenitor cells
Istiaq, Arif; Umemoto, Terumasa; Ito, Naofumi; Suda, Toshio;
Shimamura, Kenji; Ohta, Kunimasa
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 10.n/a:n/a,2022 MEDLINE:36478736 Article
219.IMPDH inhibition activates TLR-VCAM1 pathway andsuppresses the development of MLL-fusion leukemia
Liu, Xiaoxiao; Sato, Naru; Yabushita, Tomohiro; Li, Jingmei; Jia,
Yuhan; Tamura, Moe; Asada, Shuhei; Fujino, Takeshi; Fukushima,
Tsuyoshi; Yonezawa, Taishi; Tanaka, Yosuke; Fukuyama, Tomofusa;
Tsuchiya, Akiho; Shikata, Shiori; Iwamura, Hiroyuki; Kinouchi,
Chieko; Komatsu, Kensuke; Yamasaki, Satoshi; Shibata, Tatsuhiro;
Sasaki, Atsuo T.; Schibler, Janet; Wunderlich, Mark; O’Brien, Eric;
Mizukawa, Benjamin; Mulloy, James C.; Sugiura, Yuki; Takizawa,
Hitoshi; Shibata, Takuma; Miyake, Kensuke; Kitamura, Toshio;
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EMBO MOLECULAR MEDICINE 15.1:n/a,2022
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220.C A R D 11 m u t a t i o n a n d H B Z e x p r e s s i o n i n d u c elymphoproliferative disease and adult T-cell leukemia/
lymphoma
Kameda, Takuro; Shide, Kotaro; Kamiunten, Ayako; Kogure,Yasunori; Morishita, Daisuke; Koya, Junji; Tahira, Yuki; Akizuki,
Keiichi; Yokomizo-Nakano, Takako; Kubota, Sho; Marutsuka,
Kosuke; Sekine, Masaaki; Hidaka, Tomonori; Kubuki, Yoko; Kitai,
Yuichi; Matsuda, Tadashi; Yoda, Akinori; Ohshima, Takayuki;
Sugiyama, Midori; Sashida, Goro; Kataoka, Keisuke; Ogawa,Seishi; Shimoda, Kazuya
COMMUNICATIONS BIOLOGY 5.1:n/a,2022 MEDLINE:36446869
Article221.A Myb enhancer-guided analysis of basophil and mast celldifferentiation
Matsumura, Takayoshi; Totani, Haruhito; Gunji, Yoshitaka; Fukuda,
Masahiro; Yokomori, Rui; Deng, Jianwen; Rethnam, Malini; Yang,
Chong; Tan, Tze King; Karasawa, Tadayoshi; Kario, Kazuomi;
Takahashi, Masafumi; Osato, Motomi; Sanda, Takaomi; Suda,
ToshioNATURE COMMUNICATIONS 13.1:n/a,2022 MEDLINE:36400777
Article222.Iron accelerates Fusobacterium nucleatum-induced CCL8
expression in macrophages and is associated with colorectal
cancer progression
Yamane, Taishi; Kanamori, Yohei; Sawayama, Hiroshi; Yano,
Hiromu; Nita, Akihiro; Ohta, Yudai; Hinokuma, Hironori; Maeda,
Ayato; Iwai, Akiko; Matsumoto, Takashi; Shimoda, Mayuko; Niimura,
Mayumi; Usuki, Shingo; Yasuda-Yoshihara, Noriko; Niwa, Masato;Baba, Yoshifumi; Ishimoto, Takatsugu; Komohara, Yoshihiro; Sawa,
Tomohiro; Hirayama, Tasuku; Baba, Hideo; Moroishi, Toshiro
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223.Leukopenia, macrocytosis, and thrombocytopenia occur inyoung adults with Down syndrome
Hamaguchi, Yo; Kondoh, Tatsuro; Fukuda, Masafumi; Yamasaki,
Kazumi; Yoshiura, Koh-ichiro; Moriuchi, Hiroyuki; Morii, Mariko;
Muramatsu, Masashi; Minami, Takashi; Osato, Motomi
GENE 835.n/a:n/a,2022 MEDLINE:35690282 Article
224.Loss of METTL3 attenuates blastic plasmacytoid dendritic
cell neoplasm response to PRMT5 inhibition via IFN signaling
Rethnam, Malini; Tan, Darren Qiancheng; Tan, Shi Hao; Li, Jia;
Yokomori, Rui; Li, Ying; Yang, Henry; Sanda, Takaomi; Suda, Toshio
BLOOD ADVANCES 6.18:5330-5344,2022 MEDLINE:35482445
Article225.The extracellular matrix fibulin 7 maintains epidermal stem
cell heterogeneity during skin aging
Raja, Erna; Changarathil, Gopakumar; Oinam, Lalhaba; Tsunezumi,
Jun; Ngo, Yen Xuan; Ishii, Ryutaro; Sasaki, Takako; ImanakaYoshida, Kyoko; Yanagisawa, Hiromi; Sada, Aiko
EMBO REPORTS 23.12:n/a,2022 MEDLINE:36278510 Article
226.Mesothelial fusion mediates chorioallantoic membrane
formation
Nagai, Hiroki; Tanoue, Yuki; Nakamura, Tomonori; Chan,
Christopher J. J.; Yamada, Shigehito; Saitou, Mitinori; Fukuda,
Takaichi; Sheng, Guojun
PHILOSOPHICAL TRANSACTIONS OF THE ROYALSOCIETY B-BIOLOGICAL SCIENCES 377.1865:n/a,2022
MEDLINE:36252211 Article
227.Hematopoietic stem and progenitor cells integrate microbial
signals to promote post-inflammation gut tissue repair
Sezaki, Maiko; Hayashi, Yoshikazu; Nakato, Gaku; Wang, Yuxin;
Nakata, Sayuri; Biswas, Subinoy; Morishima, Tatsuya; Fakruddin,
Md; Moon, Jieun; Ahn, Soyeon; Kim, Pilhan; Miyamoto, Yuji; Baba,
Hideo; Fukuda, Shinji; Takizawa, Hitoshi
EMBO JOURNAL 41.22:n/a,2022 MEDLINE:36254590 Article
228.C hro m a t in s t ru c ture un d e rg o e s glo b a l a n d l o c a lreorganization during murine dendritic cell development andactivation
Kurotaki, Daisuke; Kikuchi, Kenta; Cui, Kairong; Kawase, Wataru;
Saeki, Keita; Fukumoto, Junpei; Nishiyama, Akira; Nagamune,
Kisaburo; Zhao, Keji; Ozato, Keiko; Rocha, Pedro P.; Tamura,
Tomohiko
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES
OF THE UNITED STATES OF AMERICA 119.34:n/a,2022
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229.Ras GTPase-activating proteins control neuronal circuit
development in barrel cortex layer 4
Rao, Madhura S. S.; Mizuno, Hiromi; Iwasato, Takuji; Mizuno,
Hidenobu
FRONTIERS IN NEUROSCIENCE 16.n/a:n/a,2022
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230.Mitochondria transfer mediates stress erythropoiesis byaltering the bioenergetic profiles of early erythroblasts
through CD47
Yang, Chong; Yokomori, Rui; Chua, Lee Hui; Tan, Shi Hao; Tan,
Darren Qiancheng; Miharada, Kenichi; Sanda, Takaomi; Suda,
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231.Independent origins of fetal liver haematopoietic stem andprogenitor cells
Yokomizo, Tomomasa; Ideue, Takako; Morino-Koga, Saori; Tham,
Cheng Yong; Sato, Tomohiko; Takeda, Naoki; Kubota, Yoshiaki;
Kurokawa, Mineo; Komatsu, Norio; Ogawa, Minetaro; Araki, Kimi;
Osato, Motomi; Suda, Toshio
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232.Independent origins of fetal liver haematopoietic stem andprogenitor cells
Yokomizo, Tomomasa; Ideue, Takako; Morino-Koga, Saori; Tham,
Cheng Yong; Sato, Tomohiko; Takeda, Naoki; Kubota, Yoshiaki;
Kurokawa, Mineo; Komatsu, Norio; Ogawa, Minetaro; Araki, Kimi;
Osato, Motomi; Suda, Toshio
NATURE 609.7928:779-+,2022 MEDLINE:36104564 Article
233.A Randomized, Double-Blind Comparison Study of Royal
Jelly to Augment Vascular Endothelial Function in Healthy
Volunteers
Fujisue, Koichiro; Yamamoto, Eiichiro; Sueta, Daisuke; Arima,
Yuichiro; Hirakawa, Kyoko; Tabata, Noriaki; Ishii, Masanobu;
Ito, Miwa; Yamanaga, Kenshi; Hanatani, Shinsuke; Hoshiyama,
Tadashi; Kanazawa, Hisanori; Takashio, Seiji; Araki, Satoshi;
Usuku, Hiroki; Nakamura, Taishi; Soejima, Hirofumi; Kaikita, Koichi;
Kawano, Hiroaki; Matsushita, Kenichi; Tsujita, Kenichi
JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS
29.9:1285-1294,2022 MEDLINE:34588374 Article
234.Tumor microenvironmental 15-PGDH depletion promotes
fibrotic tumor formation and angiogenesis in pancreaticcancer
Bu, Luke; Yonemura, Atsuko; Yasuda-Yoshihara, Noriko; Uchihara,
Tomoyuki; Ismagulov, Galym; Takasugi, Sanae; Yasuda, Tadahito;
Okamoto, Yuya; Kitamura, Fumimasa; Akiyama, Takahiko; Arima,
Kota; Itoyama, Rumi; Zhang, Jun; Fu, Lingfeng; Hu, Xichen; Wei,
Feng; Arima, Yuichiro; Moroishi, Toshiro; Nishiyama, Koichi; Sheng,
Guojun; Mukunoki, Toshifumi; Otani, Jun; Baba, Hideo; Ishimoto,Takatsugu
CANCER SCIENCE 113.10:3579-3592,2022 MEDLINE:35848891
Article235.Bone marrow imaging reveals the migration dynamics of
neonatal hematopoietic stem cells
Takihara, Yuji; Higaki, Takumi; Yokomizo, Tomomasa; Umemoto,
Terumasa; Ariyoshi, Kazunori; Hashimoto, Michihiro; Sezaki, Maiko;
Takizawa, Hitoshi; Inoue, Toshihiro; Suda, Toshio; Mizuno, Hidenobu
COMMUNICATIONS BIOLOGY 5.1:n/a,2022 MEDLINE:35918480
Article236.Validation of the obesity paradox by body mass index andwaist circumference in patients undergoing percutaneous
coronary intervention
Shirahama, Yuichiro; Tabata, Noriaki; Sakamoto, Kenji; Sato,
Ryota; Yamanaga, Kenshi; Fujisue, Koichiro; Sueta, Daisuke;
Araki, Satoshi; Takashio, Seiji; Arima, Yuichiro; Hokimoto, Seiji;
Sato, Koji; Sakamoto, Tomohiro; Nakao, Koichi; Shimomura,
Hideki; Matsumura, Toshiyuki; Tayama, Shinji; Fujimoto, Kazuteru;
Oshima, Shuichi; Nakamura, Shinichi; Tsunoda, Ryusuke; Hirose,
Toyoki; Kikuta, Koichi; Sakaino, Naritsugu; Yamamoto, Nobuyasu;
Kajiwara, Ichiro; Suzuki, Satoru; Yamamoto, Eiichiro; Kaikita, Koichi;
Matsushita, Kenichi; Tsujita, Kenichi
INTERNATIONAL JOURNAL OF OBESITY 46.10:1840-1848,2022
MEDLINE:35902692 Article
237.Malnutrition-associated high bleeding risk with low
thrombogenicity in patients undergoing percutaneous
coronary intervention
Nakanishi, Nobuhiro; Kaikita, Koichi; Ishii, Masanobu; Kuyama,
Naoto; Tabata, Noriaki; Ito, Miwa; Yamanaga, Kenshi; Fujisue,
Koichiro; Hoshiyama, Tadashi; Kanazawa, Hisanori; Hanatani,
Shinsuke; Sueta, Daisuke; Takashio, Seiji; Arima, Yuichiro;
Araki, Satoshi; Usuku, Hiroki; Nakamura, Taishi; Suzuki, Satoru;
Yamamoto, Eiichiro; Soejima, Hirofumi; Matsushita, Kenichi; Tsujita,
Kenichi
NUTRITION METABOLISM AND CARDIOVASCULARDISEASES 32.5:1227-1235,2022 MEDLINE:35197212 Article― 70 ―

238.Overexpression of HMGN3 nucleosome binding protein is
associated with tumor invasion and TGF-? expression incholangiocarcinoma
Sorin, Supannika; Pokaew, Nongnapas; Vaeteewoottacharn,
Kulthida; Waraasawapati, Sakda; Pairojkul, Chawalit; Sashida,
Goro; Sawanyawisuth, Kanlayanee
SCIENCEASIA 48.5:518-+,2022 n/a Article
239.Recent advances in? sickle and niche? research-Tribute to Dr.
Paul S Frenette
Torres, Lidiane S.; Asada, Noboru; Weiss, Mitchell J.; Trumpp,
Andreas; Suda, Toshio; Scadden, David T.; Ito, Keisuke
STEM CELL REPORTS 17.7:1509-1535,2022 MEDLINE:35830837
Review
240.Depth-targeted intracortical microstroke by two-photon
photothrombosis in rodent brain
Fukuda, Masahiro; Matsumura, Takayoshi; Suda, Toshio; Hirase,
HajimeNEUROPHOTONICS 9.2:n/a,2022 MEDLINE:35311215 Article
241.Prolonged Myocardial Regenerative Capacity in Neonatal
Opossum
Nishiyama, Chihiro; Saito, Yuichi; Sakaguchi, Akane; Kaneko,
Mari; Kiyonari, Hiroshi; Xu, Yuqing; Arima, Yuichiro; Uosaki, Hideki;
Kimura, Wataru
CIRCULATION 146.2:125-139,2022 MEDLINE:35616010 Article
242.Deficiency of the kidney tubular angiotensin II type1 receptorassociated protein ATRAP exacerbates streptozotocininduced diabetic glomerular injury via reducing protective
macrophage polarization
Haruhara, Kotaro; Suzuki, Toru; Wakui, Hiromichi; Azushima,
Kengo; Kurotaki, Daisuke; Kawase, Wataru; Uneda, Kazushi;
Kobayashi, Ryu; Ohki, Kohji; Kinguchi, Sho; Yamaji, Takahiro;
Kato, Ikuma; Ohashi, Kenichi; Yamashita, Akio; Tamura, Tomohiko;
Tsuboi, Nobuo; Yokoo, Takashi; Tamura, Kouichi
KIDNEY INTERNATIONAL 101.5:912-928,2022
MEDLINE:35240129 Article
243.Gut microbiome in gastrointestinal cancer: a friend or foe?
Liu, Yang; Baba, Yoshifumi; Ishimoto, Takatsugu; Gu, Xi; Zhang,Jun; Nomoto, Daichi; Okadome; Baba, Hideo; Qiu, Peng
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
18.10:4101-4117,2022 MEDLINE:35844804 Review
244.Unique molecular and functional features of extramedullary
hematopoietic stem and progenitor cell reservoirs in humans
Mende, Nicole; Bastos, Hugo P.; Santoro, Antonella; Mahbubani,
Krishnaa T.; Ciaurro, Valerio; Calderbank, Emily F.; Londono, Mariana
Quiroga; Sham, Kendig; Mantica, Giovanna; Morishima, Tatsuya;
Mitchell, Emily; Lidonnici, Maria Rosa; Meier-Abt, Fabienne; Hayler,
Daniel; Jardine, Laura; Curd, Abbie; Haniffa, Muzlifah; Ferrari,
Giuliana; Takizawa, Hitoshi; Wilson, Nicola K.; Gottgens, Berthold;
Saeb-Parsy, Kourosh; Frontini, Mattia; Laurenti, Elisa
BLOOD 139.23:3387-3401,2022 MEDLINE:35073399 Article
245.Prognostic value of right ventricular global longitudinal strain
in transthyretin amyloid cardiomyopathy
Usuku, Hiroki; Takashio, Seiji; Yamamoto, Eiichiro; Yamada,Toshihiro; Egashira, Koichi; Morioka, Mami; Nishi, Masato; Komorita,
Takashi; Oike, Fumi; Tabata, Noriaki; Ishii, Masanobu; Yamanaga,
Kenshi; Fujisue, Koichiro; Sueta, Daisuke; Arima, Yuichiro; Araki,
Satoshi; Oda, Seitaro; Misumi, Yohei; Kawano, Hiroaki; Matsushita,
Kenichi; Ueda, Mitsuharu; Matsui, Hirotaka; Tsujita, Kenichi
JOURNAL OF CARDIOLOGY 80.1:56-63,2022
MEDLINE:35282945 Article

246.HMGN3 represses transcription of epithelial regulators
to promote migration of cholangiocarcinoma in a SNAI2dependent manner
Sorin, Supannika; Kubota, Sho; Hamidi, Sofiane; YokomizoNakano, Takako; Vaeteewoottacharn, Kulthida; Wongkham, Sopit;
Waraasawapati, Sakda; Pairojkul, Chawalit; Bai, Jie; Morii, Mariko;
Sheng, Guojun; Sawanyawisuth, Kanlayanee; Sashida, Goro
FASEB JOURNAL 36.7:n/a,2022 MEDLINE:35635715 Article
247.Induction of enucleation in primary and immortalized
erythroid cells
Soboleva, Svetlana; Miharada, Kenichi
INTERNATIONAL JOURNAL OF HEMATOLOGY 116.2:192198,2022 MEDLINE:35610497 Article
248.Mechanical loading of intraluminal pressure mediates woundangiogenesis by regulating the TOCA family of F-BAR
proteins
Yuge, Shinya; Nishiyama, Koichi; Arima, Yuichiro; Hanada,
Yasuyuki; Oguri-Nakamura, Eri; Hanada, Sanshiro; Ishii, Tomohiro;
Wakayama, Yuki; Hasegawa, Urara; Tsujita, Kazuya; Yokokawa,
Ryuji; Miura, Takashi; Itoh, Toshiki; Tsujita, Kenichi; Mochizuki,
Naoki; Fukuhara, Shigetomo
NATURE COMMUNICATIONS 13.1:n/a,2022 MEDLINE:35551172
Article249.Targeting chemoresistance in Xp11.2 translocation renal cell
carcinoma using a novel polyamide-chlorambucil conjugate
Funasaki, Shintaro; Mehanna, Sally; Ma, Wenjuan; Nishizawa,
Hidekazu; Kamikubo, Yasuhiko; Sugiyama, Hiroshi; Ikeda, Shuji;Motoshima, Takanobu; Hasumi, Hisashi; Linehan, W. Marston;
Schmidt, Laura S.; Ricketts, Chris; Suda, Toshio; Oike, Yuichi;
Kamba, Tomomi; Baba, Masaya
CANCER SCIENCE 113.7:2352-2367,2022 MEDLINE:35396773
Article250.Fusobacterium nucleatum promotes esophageal squamouscell carcinoma progression via the NOD1/RIPK2/NF- ﾈB
pathway
Nomoto, Daichi; Baba, Yoshifumi; Liu, Yang; Tsutsuki, Hiroyasu;
Okadome, Kazuo; Harada, Kazuto; Ishimoto, Takatsugu; Iwatsuki,
Masaaki; Iwagami, Shiro; Miyamoto, Yuji; Yoshida, Naoya;
Watanabe, Masayuki; Moroishi, Toshiro; Komohara, Yoshihiro;
Sawa, Tomohiro; Baba, Hideo
CANCER LETTERS 530.n/a:59-67,2022 MEDLINE:35033591
Article251.Multidimensional Dynamics and Forecast Models of Network
Public Opinions Based on the Fusion of Smart Transportation
and Big Data
Sheng, Guojun; Guan, Yi
JOURNAL OF ADVANCED TRANSPORTATION 2022.n/a:n/a,2022
n/a Article
252.Live imaging of avian epiblast and anterior mesendoderm
grafting reveals the complexity of cell dynamics during earlybrain development
Yoshihi, Koya; Kato, Kagayaki; Iida, Hideaki; Teramoto, Machiko;
Kawamura, Akihito; Watanabe, Yusaku; Nunome, Mitsuo; Nakano,
Mikiharu; Matsuda, Yoichi; Sato, Yuki; Mizuno, Hidenobu; Iwasato,
Takuji; Ishii, Yasuo; Kondoh, Hisato
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253.Utility of left atrial and ventricular strain for diagnosis oftransthyretin amyloid cardiomyopathy in aortic stenosis
Oike, Fumi; Usuku, Hiroki; Yamamoto, Eiichiro; Marume, Kyohei;
Takashio, Seiji; Ishii, Masanobu; Tabata, Noriaki; Fujisue, Koichiro;
Yamanaga, Kenshi; Sueta, Daisuke; Hanatani, Shinsuke; Arima,
Yuichiro; Araki, Satoshi; Oda, Seitaro; Kawano, Hiroaki; Soejima,
Hirofumi; Matsushita, Kenichi; Ueda, Mitsuharu; Fukui, Toshihiro;
Tsujita, Kenichi
ESC HEART FAILURE 9.3:1976-1986,2022 MEDLINE:35338611
Article254.Preoperative skeletal muscle status is associated with tumorinfiltrating lymphocytes and prognosis in patients withcolorectal cancer
Daitoku, Nobuya; Miyamoto, Yuji; Hiyoshi, Yukiharu; Tokunaga,
Ryuma; Sakamoto, Yuki; Sawayama, Hiroshi; Ishimoto, Takatsugu;
Baba, Yoshifumi; Yoshida, Naoya; Baba, Hideo
ANNALS OF GASTROENTEROLOGICAL SURGERY 6.5:658666,2022 MEDLINE:36091309 Article
255.The Gut Microbiota in Inflammatory Bowel Disease
Qiu, Peng; Ishimoto, Takatsugu; Fu, Lingfeng; Zhang, Jun; Zhang,
Zhenyong; Liu, Yang
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
12.n/a:n/a,2022 MEDLINE:35273921 Review
256.Single-Cell Atlas of Lineage States, Tumor Microenvironment,
and Subtype-Specific Expression Programs in Gastric Cancer
Kumar, Vikrant; Ramnarayanan, Kalpana; Sundar, Raghav;
Padmanabhan, Nisha; Srivastava, Supriya; Koiwa, Mayu; Yasuda,
Tadahito; Koh, Vivien; Huang, Kie Kyon; Tay, Su Ting; Ho, Shamaine
Wei Ting; Tan, Angie Lay Keng; Ishimoto, Takatsugu; Kim, Guowei;
Shabbir, Asim; Chen, Qingfeng; Zhang, Biyan; Xu, Shengli; Lam,
Kong-Peng; Lum, Huey Yew Jeffrey; Teh, Ming; Yong, Wei Peng;
So, Jimmy Bok Yan; Tan, Patrick
CANCER DISCOVERY 12.3:670-691,2022 MEDLINE:34642171
Article257.ATP citrate lyase controls hematopoietic stem cell fate andsupports bone marrow regeneration
Umemoto, Terumasa; Johansson, Alban; Ahmad, Shah Adil
Ishtiyaq; Hashimoto, Michihiro; Kubota, Sho; Kikuchi, Kenta; Odaka,
Haruki; Era, Takumi; Kurotaki, Daisuke; Sashida, Goro; Suda, Toshio
EMBO JOURNAL 41.8:n/a,2022 MEDLINE:35229328 Article
258.Inflammation Regulates Haematopoietic Stem Cells and Their
Niche
Ho, Nicole Pui-Yu; Takizawa, Hitoshi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
23.3:n/a,2022 MEDLINE:35163048 Review
259.Bivalent-histone-marked immediate-early gene regulation isvital for VEGF-responsive angiogenesis
Kanki, Yasuharu; Muramatsu, Masashi; Miyamura, Yuri; Kikuchi,
Kenta; Higashijima, Yoshiki; Nakaki, Ryo; Suehiro, Jun-ichi; Sasaki,
Yuji; Kubota, Yoshiaki; Koseki, Haruhiko; Morioka, Hiroshi; Kodama,
Tatsuhiko; Nakao, Mitsuyoshi; Kurotaki, Daisuke; Aburatani,
Hiroyuki; Minami, Takashi
CELL REPORTS 38.6:n/a,2022 MEDLINE:35139389 Article

261.Increased soluble programed cell death-ligand 1 is associatedwith acute coronary syndrome
Fujisue, Koichiro; Yamamoto, Eiichiro; Sueta, Daisuke; Takae,
Masafumi; Nishihara, Taiki; Komorita, Takashi; Usuku, Hiroki;
Yamanaga, Kenshi; Ito, Miwa; Hoshiyama, Tadashi; Kanazawa,
Hisanori; Takashio, Seiji; Arima, Yuichiro; Araki, Satoshi; Soejima,
Hirofumi; Kaikita, Koichi; Matsushita, Kenichi; Tsujita, Kenichi
INTERNATIONAL JOURNAL OF CARDIOLOGY 349.n/a:6Jan,2022 MEDLINE:34843822 Article
262.Clinicopathological characteristics and prognosis of poorly
cohesive cell subtype of gastric cancer
Nakamura, Kenichi; Eto, Kojiro; Iwagami, Shiro; Ogawa, Katsuhiro;
Sawayama, Hiroshi; Ishimoto, Takatsugu; Iwatsuki, Masaaki; Baba,
Yoshifumi; Miyamoto, Yuji; Yoshida, Naoya; Baba, Hideo
INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
27.3:512-519,2022 MEDLINE:35084597 Article
263.Transcriptomic analysis of functional diversity of humanumbilical cord blood hematopoietic stem/progenitor cells inerythroid differentiation
Soboleva, Svetlana; Akerstrand, Hugo; Miharada, Kenichi
INTERNATIONAL JOURNAL OF HEMATOLOGY 115.4:481488,2022 MEDLINE:35088351 Article
264.CD244 expression represents functional decline of murine
hematopoietic stem cells after in vitro culture
Koide, Shuhei; Sigurdsson, Valgardur; Radulovic, Visnja; Saito,
Kiyoka; Zheng, Zhiqian; Lang, Stefan; Soneji, Shamit; Iwama,
Atsushi; Miharada, Kenichi
ISCIENCE 25.1:n/a,2022 MEDLINE:35005548 Article
265.The acidic domain of Hmga2 and the domain’s linker region
are critical for driving self-renewal of hematopoietic stem cellSun, Yuqi; Kubota, Sho; Iimori, Mihoko; Hamashima, Ai; Murakami,
Haruka; Bai, Jie; Morii, Mariko; Yokomizo-Nakano, Takako; Osato,
Motomi; Araki, Kimi; Sashida, Goro
INTERNATIONAL JOURNAL OF HEMATOLOGY 115.4:553562,2022 MEDLINE:35067851 Article
266.IL-1 mediates microbiome-induced inflammaging ofhematopoietic stem cells in mice
Kovtonyuk, Larisa, V; Caiado, Francisco; Garcia-Martin, Santiago;
Manz, Eva-Maria; Helbling, Patrick; Takizawa, Hitoshi; Boettcher,
Steffen; Al-Shahrour, Fatima; Nombela-Arrieta, Cesar; Slack,
Emma; Manz, Markus G.
BLOOD 139.1:44-58,2022 MEDLINE:34525198 Article
267.Eliminating chronic myeloid leukemia stem cells by IRAK1/4
inhibitors
Tanaka, Yosuke; Takeda, Reina; Fukushima, Tsuyoshi; Mikami,
Keiko; Tsuchiya, Shun; Tamura, Moe; Adachi, Keito; Umemoto,
Terumasa; Asada, Shuhei; Watanabe, Naoki; Morishita, Soji;
Imai, Misa; Nagata, Masayoshi; Araki, Marito; Takizawa, Hitoshi;
Fukuyama, Tomofusa; Lamagna, Chrystelle; Masuda, Esteban S.;
Ito, Ryoji; Goyama, Susumu; Komatsu, Norio; Takaku, Tomoiku;
Kitamura, Toshio
NATURE COMMUNICATIONS 13.1:n/a,2022 MEDLINE:35022428
Article260.Intracellular MUC20 variant 2 maintains mitochondrial calcium
homeostasis and enhances drug resistance in gastric cancer
Fu, Lingfeng; Yonemura, Atsuko; Yasuda-Yoshihara, Noriko;Umemoto, Terumasa; Zhang, Jun; Yasuda, Tadahito; Uchihara,
Tomoyuki; Akiyama, Takahiko; Kitamura, Fumimasa; Yamashita,
Kohei; Okamoto, Yuya; Bu, Luke; Wei, Feng; Hu, Xichen; Liu, Yang;
Ajani, Jaffer A.; Tan, Patrick; Baba, Hideo; Ishimoto, Takatsugu
GASTRIC CANCER 25.3:542-557,2022 MEDLINE:35166958
Article― 72 ―

268.Impact of cerebrovascular comorbidity on prognosis inJapanese patients undergoing PCI: 1-year data from Japanesemulticenter registry (KICS)
Ahmed, Khlood; Arima, Yuichiro; Tabata, Noriaki; Ishii, Masanobu;
Sato, Ryota; Yamashita, Takayoshi; Yamanaga, Kenshi; Takizawa,
Hitoshi; Hokimoto, Seiji; Sueta, Daisuke; Araki, Satoshi; Fujisue,
Koichiro; Takashio, Seiji; Fujimoto, Kazuteru; Shimomura, Hideki;
Tsunoda, Ryusuke; Hirose, Toyoki; Sato, Koji; Kikuta, Koichi;
Sakaino, Naritsugu; Nakamura, Shinichi; Yamamoto, Nobuyasu;
Matsumura, Toshiyuki; Kajiwara, Ichiro; Tayama, Shinji; Sakamoto,Tomohiro; Nakao, Koichi; Oshima, Shuichi; Yamamoto, Eiichiro;
Sakamoto, Kenji; Kaikita, Koichi; Matsushita, Kenichi; Tsujita,
Kenichi
HEART AND VESSELS 37.6:911-918,2022 MEDLINE:35013771
Article269.Impact of cerebrovascular comorbidity on prognosis inJapanese patients undergoing PCI: 1-year data from Japanesemulticenter registry (KICS)
Ahmed, Khlood; Arima, Yuichiro; Tabata, Noriaki; Ishii, Masanobu;
Sato, Ryota; Yamashita, Takayoshi; Yamanaga, Kenshi; Takizawa,
Hitoshi; Hokimoto, Seiji; Sueta, Daisuke; Araki, Satoshi; Fujisue,
Koichiro; Takashio, Seiji; Fujimoto, Kazuteru; Shimomura, Hideki;
Tsunoda, Ryusuke; Hirose, Toyoki; Sato, Koji; Kikuta, Koichi;
Sakaino, Naritsugu; Nakamura, Shinichi; Yamamoto, Nobuyasu;
Matsumura, Toshiyuki; Kajiwara, Ichiro; Tayama, Shinji; Sakamoto,Tomohiro; Nakao, Koichi; Oshima, Shuichi; Yamamoto, Eiichiro;
Sakamoto, Kenji; Kaikita, Koichi; Matsushita, Kenichi; Tsujita,
Kenichi
HEART AND VESSELS 37.6:911-918,2022 MEDLINE:35013771
Article270.MEK inhibition suppresses metastatic progression of KRASmutated gastric cancer
Yamasaki, Juntaro; Hirata, Yuki; Otsuki, Yuji; Suina, Kentaro; Saito,
Yoshiyuki; Masuda, Kenta; Okazaki, Shogo; Ishimoto, Takatsugu;
Saya, Hideyuki; Nagano, Osamu
CANCER SCIENCE 113.3:916-925,2022 MEDLINE:34931404
Article271.GPRC5C drives branched-chain amino acid metabolism inleukemogenesis
Zhang, Yu Wei; Mess, Julian; Lalioti, Maria-Eleni; Romero-Mulero,
Mari Carmen; Velasco-Hernandez, Talia; Obier, Nadine; Karantzelis,
Nikolaos; Rettkowski, Jasmin; Schoenberger, Katharina; Karabacz,
Noemie; Jaecklein, Karin; Morishima, Tatsuya; Trincado, Juan Luis;
Romecin, Paola; Martinez, Alba; Takizawa, Hitoshi; Shoumariyeh,
Khalid; Renders, Simon; Zeiser, Robert; Pahl, Heike L.; Beliveau,
Francois; Hebert, Josee; Lehnertz, Bernhard; Sauvageau, Guy;
Menendez, Pablo; Cabezas-Wallscheid, Nina
BLOOD ADVANCES 7.24:7525-7538,2023 MEDLINE:37639313
Article272.The Impact of Ketone Body Metabolism on Mitochondrial
Function and Cardiovascular Diseases
Arima, Yuichiro
JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS
30.12:1751-1758,2023 MEDLINE:37766574 Review

273.A Bird’s-Eye View of Endangered Species Conservation:
Avian Genomics and Stem Cell Approaches for Green
Peafowl (Pavo muticus)
Intarapat, Sittipon; Sukparangsi, Woranop; Gusev, Oleg; Sheng,
Guojun
GENES 14.11:n/a,2023 MEDLINE:38002983 Review

274.Loss of endothelial membrane KIT ligand affects systemic
KIT ligand levels but not bone marrow hematopoietic stem
cells
Matsuoka, Sahoko; Facchini, Raffaella; Luis, Tiago C.; Carrelha,
Joana; Woll, Petter S.; Mizukami, Takuo; Wu, Bishan; Boukarabila,
Hanane; Buono, Mario; Norfo, Ruggiero; Arai, Fumio; Suda, Toshio;
Mead, Adam J.; Nerlov, Claus; Jacobsen, Sten Eirik W.
BLOOD 142.19:1622-1632,2023 MEDLINE:37562000 Article
275.Functional and molecular profiling of hematopoietic stem
cells during regeneration
Rydstrom, Anna; Grahn, Tan H. M.; Niroula, Abhishek; Mansell,Els; Van der Garde, Mark; Pertesi, Maroulio; Subramaniam,
Agatheeswaran; Soneji, Shamit; Zubarev, Roman; Enver, Tariq;
Nilsson, Bjorn; Miharada, Kenichi; Larsson, Jonas; Karlssona,
StefanEXPERIMENTAL HEMATOLOGY 127.n/a:40-51,2023
MEDLINE:37666355 Article
276.Phospholipid metabolic adaptation promotes survival of IDH2
mutant acute myeloid leukemia cells
Morishima, Tatsuya; Takahashi, Koichi; Chin, Desmond Wai Loon;
Wang, Yuxin; Tokunaga, Kenji; Arima, Yuichiro; Matsuoka, Masao;
Suda, Toshio; Takizawa, Hitoshi
CANCER SCIENCE n/a.n/a:n/a,2023 MEDLINE:37882467 Article
277.Akkermansia muciniphila induces slow extramedullary
hematopoiesis via cooperative IL-1R/TLR signals
Wang, Yuxin; Morishima, Tatsuya; Sezaki, Maiko; Sato, Ryo;
Nakato, Gaku; Fukuda, Shinji; Kobiyama, Kouji; Ishii, Ken J.; Li,
Yuhua; Takizawa, Hitoshi
EMBO REPORTS n/a.n/a:n/a,2023 MEDLINE:37870318 Article
278.MBTD1 preserves adult hematopoietic stem cell pool size andfunction
Takubo, Keiyo; Htun, Phyo Wai; Ueda, Takeshi; Sera, Yasuyuki;
Iwasaki, Masayuki; Koizumi, Miho; Shiroshita, Kohei; Kobayashi,
Hiroshi; Haraguchi, Miho; Watanuki, Shintaro; Honda, Zen-ichiro;
Yamasaki, Norimasa; Ishizu, Ayako Nakamura-; Arai, Fumio;
Motoyama, Noboru; Hatta, Tomohisa; Natsume, Tohru; Suda,
Toshio; Honda, Hiroaki
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES
OF THE UNITED STATES OF AMERICA 120.32:n/a,2023
MEDLINE:37523546 Article
279.Prognostic Significance of Soluble PD-L 1 on Cardiovascular
Outcomes in Patients with Coronary Artery DiseaseMiyazaki, Shuhei; Fujisue, Koichiro; Yamanaga, Kenshi; Sueta,
Daisuke; Usuku, Hiroki; Tabata, Noriaki; Ishii, Masanobu; Hanatani,
Shinsuke; Hoshiyama, Tadashi; Kanazawa, Hisanori; Takashio, Seiji;
Arima, Yuichiro; Araki, Satoshi; Yamamoto, Eiichiro; Matsushita,
Kenichi; Tsujita, Kenichi
JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS n/a.n/
a:n/a,2023 MEDLINE:37793811 Article
280.T1a Renal Cell Carcinoma With Metastasis: Japanese Societyof Renal Cancer Retrospective Multi-institute Results
Kato, Taigo; Wang, Cong; Masumori, Naoya; Yamasaki, Toshinari;Matsumoto, Hiroaki; Baba, Masaya; Ito, Keiichi; Kimura, Takahiro;
Kitamura, Hiroshi; Takahashi, Masayuki; Hongo, Fumiya; Hasine,
Katsuyoshi; Eto, Masatoshi; Ikeda, Jun-ichiro; Nonomura, Norio;
Shinohara, Nobuo
ANTICANCER RESEARCH 43.9:4061-4065,2023
MEDLINE:37648301 Article
281.Dosage compensation of Z sex chromosome genes in avian
fibroblast cells
Deviatiiarov, Ruslan; Nagai, Hiroki; Ismagulov, Galym; Stupina,
Anastasia; Wada, Kazuhiro; Ide, Shinji; Toji, Noriyuki; Zhang, Heng;
Sukparangsi, Woranop; Intarapat, Sittipon; Gusev, Oleg; Sheng,
GuojunGENOME BIOLOGY 24.1:n/a,2023 MEDLINE:37730643 Article― 73 ―

282.A stepwise and digital pattern of RSK phosphorylation
determines the outcome of thymic selection
Funasaki, Shintaro; Hatano, Atsushi; Nakatsumi, Hirokazu; Koga,
Daisuke; Sugahara, Osamu; Yumimoto, Kanae; Baba, Masaya;
Matsumoto, Masaki; Nakayama, Keiichi I.
ISCIENCE 26.9:n/a,2023 MEDLINE:37646020 Article
283.ANGPTL2-mediated epigenetic repression of MHC-I in tumor
cells accelerates tumor immune evasion
Kadomatsu, Tsuyoshi; Hara, Chiaki; Kurahashi, Ryoma; Horiguchi,
Haruki; Morinaga, Jun; Miyata, Keishi; Kurano, Sohtaro; Kanemaru,
Hisashi; Fukushima, Satoshi; Araki, Kimi; Baba, Masaya; Linehan, W.
Marston; Kamba, Tomomi; Oike, Yuichi
MOLECULAR ONCOLOGY 17.12:2637-2658,2023
MEDLINE:37452654 Article
284.Restoring tumor immunogenicity with dendritic cell
reprogramming
Zimmermannova, Olga; Ferreira, Alexandra G.; Ascic, Ervin;
Santiago, Marta Velasco; Kurochkin, Ilia; Hansen, Morten; Met,
Ozcan; Caiado, Ines; Shapiro, Ilja E.; Michaux, Justine; Humbert,
Marion; Soto-Cabrera, Diego; Benonisson, Hreinn; SilverioAlves, Rita; Gomez-Jimenez, David; Bernardo, Carina; Bauden,
Monika; Andersson, Roland; Hoglund, Mattias; Miharada, Kenichi;
Nakamura, Yukio; Hugues, Stephanie; Greiff, Lennart; Lindstedt,
Malin; Rosa, Fabio F.; Pires, Cristiana F.; Bassani-Sternberg, Michal;
Svane, Inge Marie; Pereira, Carlos-Filipe
SCIENCE IMMUNOLOGY 8.85:n/a,2023 MEDLINE:37418548
Article285.Differential effects of overweight/obesity depending on
the severity of heart failure complicating acute myocardial
infarction in Japan
Matsushita, Kenichi; Kojima, Sunao; Hirakawa, Kyoko; Tabata,
Noriaki; Ito, Miwa; Yamanaga, Kenshi; Fujisue, Koichiro; Hoshiyama,
Tadashi; Hanatani, Shinsuke; Sueta, Daisuke; Kanazawa, Hisanori;
Takashio, Seiji; Arima, Yuichiro; Araki, Satoshi; Usuku, Hiroki;
Suzuki, Satoru; Yamamoto, Eiichiro; Nakamura, Taishi; Soejima,
Hirofumi; Kaikita, Koichi; Tsujita, Kenichi
PROGRESS IN CARDIOVASCULAR DISEASES 78.n/a:4957,2023 MEDLINE:36481210 Review
286.Inositol pyrophosphate profiling reveals regulatory roles of
IP6K2-dependent enhanced IP7 metabolism in the entericnervous system
Ito, Masatoshi; Fujii, Natsuko; Kohara, Saori; Hori, Shuho; Tanaka,
Masayuki; Wittwer, Christopher; Kikuchi, Kenta; Iijima, Takatoshi;
Kakimoto, Yu; Hirabayashi, Kenichi; Kurotaki, Daisuke; Jessen,
Henning J.; Saiardi, Adolfo; Nagata, Eiichiro
JOURNAL OF BIOLOGICAL CHEMISTRY 299.3:n/a,2023
MEDLINE:36681123 Article
287.Comparative analyses define differences between BHDassociated renal tumour and sporadic chromophobe renal
cell carcinoma
Jikuya, Ryosuke; Johnson, Todd A.; Maejima, Kazuhiro; An, Jisong;
Ju, Young-Seok; Lee, Hwajin; Ha, Kyungsik; Song, Woojeung;
Kim, Youngwook; Okawa, Yuki; Sasagawa, Shota; Kanazashi,
Yuki; Fujita, Masashi; Imoto, Seiya; Mitome, Taku; Ohtake, Shinji;
Noguchi, Go; Kawaura, Sachi; Iribe, Yasuhiro; Aomori, Kota;
Tatenuma, Tomoyuki; Komeya, Mitsuru; Ito, Hiroki; Ito, Yusuke;
Muraoka, Kentaro; Furuya, Mitsuko; Kato, Ikuma; Fujii, Satoshi;
Hamanoue, Haruka; Tamura, Tomohiko; Baba, Masaya; Suda,
Toshio; Kodama, Tatsuhiko; Makiyama, Kazuhide; Yao, Masahiro;
Shuch, Brian M.; Ricketts, Christopher J.; Schmidt, Laura S.;
Linehan, W. Marston; Nakagawa, Hidewaki; Hasumi, Hisashi
EBIOMEDICINE 92.n/a:n/a,2023 MEDLINE:37182269 Article

288.High-throughput telomere length measurement at nucleotide
resolution using the PacBio high fidelity sequencing platform
Tham, Cheng-Yong; Poon, LaiFong; Yan, TingDong; Koh, Javier
Yu Peng; Ramlee, Muhammad Khairul; Teoh, Vania Swee Imm;
Zhang, Suihan; Cai, Yi; Hong, Zebin; Lee, Gina S. S.; Liu, Jin; Song,Hai Wei; Hwang, William Ying Khee; Teh, Bin Tean; Tan, Patrick; Xu,
Lifeng; Koh, Angela S.; Osato, Motomi; Li, Shang
NATURE COMMUNICATIONS 14.1:n/a,2023 MEDLINE:36650155
Article289.IL-6 Levels Correlate with Prognosis and Immunosuppressive
Stromal Cells in Patients with Colorectal Cancer
Yamamoto, Tsunenori; Tsunedomi, Ryouichi; Nakajima, Masao;
Suzuki, Nobuaki; Yoshida, Shin; Tomochika, Shinobu; Xu, Ming;
Nakagami, Yuki; Matsui, Hiroto; Tokumitsu, Yukio; Shindo, Yoshitaro;
Watanabe, Yusaku; Iida, Michihisa; Takeda, Shigeru; Hazama,
Shoichi; Tanabe, Tsuyoshi; Ioka, Tatsuya; Hoshii, Yoshinobu; Kiyota,
Akifumi; Takizawa, Hitoshi; Kawakami, Yutaka; Ueno, Tomio;
Nagano, Hiroaki
ANNALS OF SURGICAL ONCOLOGY n/a.n/a:n/a,2023
MEDLINE:37222942 Article
290.Genomic approaches to identify and investigate genes
associated with atrial fibrillation and hear t failure
susceptibility
Patel, Kush Ketan; Venkatesan, Cynthia; Abdelhalim, Habiba;
Zeeshan, Saman; Arima, Yuichiro; Linna-Kuosmanen, Suvi; Ahmed,
Zeeshan
HUMAN GENOMICS 17.1:n/a,2023 MEDLINE:37270590 Review
291.A n inn o v a t i o n s e r v i c e s y s t e m a n d p e r s o n a li z e drecommendation for customer-product interaction life cycle insmart product service system
Zhang, Xianyu; Chen, Lucheng; Sheng, Guojun; Lu, Xiaoping;Ming, Xinguo
JOURNAL OF CLEANER PRODUCTION 398.n/a:n/a,2023 n/a
Article292.Exposure to microbial products followed by loss of Tet2
promotes myelodysplastic syndrome via remodeling HSCs
Yokomizo-Nakano, Takako; Hamashima, Ai; Kubota, Sho; Bai, Jie;
Sorin, Supannika; Sun, Yuqi; Kikuchi, Kenta; Iimori, Mihoko; Morii,
Mariko; Kanai, Akinori; Iwama, Atsushi; Huang, Gang; Kurotaki,
Daisuke; Takizawa, Hitoshi; Matsui, Hirotaka; Sashida, Goro
JOURNAL OF EXPERIMENTAL MEDICINE 220.7:n/a,2023
MEDLINE:37071125 Article
293.Stromal Reprogramming through Dual PDGFRa/b Blockade
Boosts the Efficacy of Anti-PD-1 in Fibrotic Tumors
Akiyama, Takahiko; Yasuda, Tadahito; Uchihara, Tomoyuki;
Yasuda-Yoshihara, Noriko; Tan, Benjy J. Y.; Yonemura, Atsuko;
Semba, Takashi; Yamasaki, Juntaro; Komohara, Yoshihiro; Ohnishi,
Koji; Wei, Feng; Fu, Lingfeng; Zhang, Jun; Kitamura, Fumimasa;
Yamashita, Kohei; Eto, Kojiro; Iwagami, Shiro; Tsukamoto, Hirotake;Umemoto, Terumasa; Masuda, Mari; Nagano, Osamu; Satou,
Yorifumi; Saya, Hideyuki; Tan, Patrick; Baba, Hideo; Ishimoto,Takatsugu
CANCER RESEARCH 83.5:753-770,2023 MEDLINE:36543251
Article294.Stem cell regulation and dynamics in myeloid malignancies
Sashida, Goro
INTERNATIONAL JOURNAL OF HEMATOLOGY 117.6:789790,2023 MEDLINE:37191835 Article― 74 ―

295.Humanized mouse models with endogenously developed
human natural killer cells for in vivo immunogenicity testingof HLA class I- edited iPSC-derived cells
Flahou, Charlotte; Morishima, Tatsuya; Higashi, Natsumi; Hayashi,
Yoshikazu; Xu, Huaigeng; Wang, Bo; Zhang, Chaoqi; Ninomiya,Atsushi; Qiu, Wei -Yin; Yuzuriha, Akinori; Suzuki, Daisuke;
Nakamura, Sou; Manz, Markus; Kaneko, Shin; Hotta, Akitsu;
Takizawa, Hitoshi; Eto, Koji; Sugimoto, Naoshi
BIOCHEMICAL AND BIOPHYSICAL RESEARCHCOMMUNICATIONS 662.n/a:76-83,2023 MEDLINE:37099813
Article296.Childhood hematopoietic stem cells constitute the permissive
window for RUNX1-ETO leukemogenesis
Abdallah, Mohamed Gaber; Teoh, Vania Swee Imm; Dutta, Bibek;
Yokomizo, Tomomasa; Osato, Motomi
INTERNATIONAL JOURNAL OF HEMATOLOGY 117.6:830838,2023 MEDLINE:37129801 Article
297.The Significance of SPP1 in Lung Cancers and Its Impact as aMarker for Protumor Tumor-Associated Macrophages
Matsubara, Eri; Yano, Hiromu; Pan, Cheng; Komohara, Yoshihiro;
Fujiwara, Yukio; Zhao, Shukang; Shinchi, Yusuke; Kurotaki,
Daisuke; Suzuki, Makoto
CANCERS 15.8:n/a,2023 MEDLINE:37190178 Review
298.Clinical, electrocardiographic, and echocardiographic
parameters associated with the development of pacing andimplantable cardioverter-defibrillator indication in patientswith transthyretin amyloid cardiomyopathy
Kawahara, Yusei; Kanazawa, Hisanori; Takashio, Seiji; Tsuruta,
Yuichiro; Sumi, Hitoshi; Kiyama, Takuya; Kaneko, Shozo; Ito,
Miwa; Hoshiyama, Tadashi; Hirakawa, Kyoko; Ishii, Masanobu;
Tabata, Noriaki; Yamanaga, Kenshi; Fujisue, Koichiro; Hanatani,
Shinsuke; Sueta, Daisuke; Arima, Yuichiro; Araki, Satoshi; Usuku,
Hiroki; Nakamura, Taishi; Yamamoto, Eiichiro; Soejima, Hirofumi;
Matsushita, Kenichi; Kawano, Hiroaki; Tsujita, Kenichi
EUROPACE 25.5:n/a,2023 MEDLINE:37099643 Article

302.The early neutrophil-committed progenitors aberrantly
differentiate into immunoregulatory monocytes during
emergency myelopoiesis
Ikeda, Naoki; Kubota, Hiroaki; Suzuki, Risa; Morita, Mitsuki;
Yoshimura, Ayana; Osada, Yuya; Kishida, Keigo; Kitamura, Daiki;
Iwata, Ayaka; Yotsumoto, Satoshi; Kurotaki, Daisuke; Nishimura,
Koutarou; Nishiyama, Akira; Tamura, Tomohiko; Kamatani, Takashi;
Tsunoda, Tatsuhiko; Murakawa, Miyako; Asahina, Yasuhiro;
Hayashi, Yoshihiro; Harada, Hironori; Harada, Yuka; Yokota,
Asumi; Hirai, Hideyo; Seki, Takao; Kuwahara, Makoto; Yamashta,
Masakatsu; Shichino, Shigeyuki; Tanaka, Masato; Asano, Kenichi
CELL REPORTS 42.3:n/a,2023 MEDLINE:36862552 Article
303.Involvement of protumor macrophages in breast cancer
progression and characterization of macrophage phenotypes
Komohara, Yoshihiro; Kurotaki, Daisuke; Tsukamoto, Hirotake;Miyasato, Yuko; Yano, Hiromu; Pan, Cheng; Yamamoto, Yutaka;
Fujiwara, Yukio
CANCER SCIENCE 114.6:2220-2229,2023 MEDLINE:36748310
Review
304.Multivalent mannose-conjugated siRNA causes robust genesilencing in pancreatic macrophages in vivo
Yamazaki, Kazuto; Kubara, Kenji; Suzuki, Yuta; Hihara, Taro;
Kurotaki, Daisuke; Tamura, Tomohiko; Ito, Masashi; Tsukahara,
KappeiEUROPEAN JOURNAL OF PHARMACEUTICS AND
BIOPHARMACEUTICS 183.n/a:61-73,2023 MEDLINE:36603692
Article305.MiT/TFE Family Renal Cell Carcinoma
Tang, Jinglong; Baba, Masaya
GENES 14.1:n/a,2023 MEDLINE:36672892 Review
306.The RUNX Family, a Novel Multifaceted Guardian of the
GenomeDutta, Bibek; Osato, Motomi
CELLS 12.2:n/a,2023 MEDLINE:36672189 Review

299.TFE3-immunopositive papillary renal cell carcinoma: A
clinicopathological, immunohistochemical, and genetic study
Takamatsu, Dai; Kohashi, Kenichi; Kiyozawa, Daisuke; Kinoshita,
Fumio; Ieiri, Kosuke; Baba, Masaya; Eto, Masatoshi; Oda, Yoshinao
PATHOLOGY RESEARCH AND PRACTICE 242.n/a:n/a,2023
MEDLINE:36669395 Article
300.MAC-1 marks a quiescent and functionally superior HSC
subset during regeneration
Rydstrom, Anna; Mansell, Els; Sigurdsson, Valgardur; Sjoberg,
Julia; Soneji, Shamit; Miharada, Kenichi; Larsson, Jonas
STEM CELL REPORTS 18.3:736-748,2023 MEDLINE:36868231
Article301.A gain-of-function mutation in microRNA 142 is sufficient tocause the development of T-cell leukemia in mice
Kawano, Shingo; Araki, Kimi; Bai, Jie; Furukawa, Imari; Tateishi,
Keigo; Yoshinobu, Kumiko; Usuki, Shingo; Nimmo, Rachael A.;
Kaname, Tadashi; Yoshihara, Masaharu; Takahashi, Satoru;
Sashida, Goro; Araki, Masatake
CANCER SCIENCE 114.7:2821-2834,2023 MEDLINE:36945113
Article― 75 ―

8. Appendix

8-5. List of Grants

Akaeda Medical Research Foundation（赤枝医学研究財団）
Astellas Foundation for Research on Metabolic Disorders（アステラス病態代謝研究会）
Chikumashobo Ltd.（株式会社筑摩書房）
Chugai Foundation for Innovative Drug Discovery Science (C-FINDs)（中外創薬科学財団）
Daiichi Sankyo Foundation of Life Science（第一三共生命科学研究振興財団）
Foundation for Promotion of Cancer Research（がん研究振興財団）
Friends of Leukemia Research Fund（NPO 法人白血病研究基金を育てる会）
G-7 Scholarship Foundation（G-7 奨学財団）
Hoyu Science Foundation（ホーユー科学財団）
Inoue Foundation for Science（井上科学振興財団）
Japan Agency for Medical Research and Development (AMED)（日本医療研究開発機構）
Japan Cardiovascular Research Foundation（循環器病研究振興財団）
Japan Research Foundation for Clinical Pharmacology（臨床薬理研究振興財団）
Japan Science and Technology Agency (JST)（科学技術振興機構）
Japanese Society of Hematology（日本血液学会）
Kanae Foundation for the Promotion of Medical Science（かなえ医薬振興財団）
Kato Memorial Bioscience Foundation（加藤記念バイオサイエンス振興財団）
Kobayashi Foundation for Cancer Research（小林がん学術振興会）
KOSÉ Cosmetology Research Foundation（コーセーコスメトロジー研究財団）
Kowa Life Science Foundation（興和生命科学振興財団）
Koyanagi Foundation（小柳財団）
Kyutec（ふくおかフィナンシャルグループ企業育成財団）
Ministry of Education, Culture, Sports, Science and Technology (MEXT)（文部科学省）
Ministry of Health, Labour and Welfare (MHLW)（厚生労働省）
Mochida Memorial Foundation for Medical and Pharmaceutial Research（持田記念医学薬学振興財団）
Mother and Child Health Foundation（母子健康協会）
Nagao Research Fund.（永尾武難病研究基金）
Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering（中谷医工計測技術振興財団）
Narishige Neuroscience Research Foundation（成茂神経科学研究助成基金）
Nippon Genetics Co., Ltd.（日本ジェネティクス株式会社）
Novartis Pharma K.K.（ノバルティスファーマ株式会社）
Ono Medical Research Foundation（小野医学研究財団）
Organization of the Smoking Research Foundation.（喫煙科学研究財団）
Princess Takamatsu Cancer Research Fund.（高松宮妃癌研究基金）
Research Foundation for Opto-Science and Technology（光科学技術研究振興財団）
Senri Life Science Foundation（千里ライフサイエンス振興財団）
SENSHIN Medical Research Foundation（先進医薬研究振興財団）
SGH Foundation（SGH 財団）
Shin-Nihon Senshin Foundation.（新日本先進医療研究財団）
Suntory Foundation for Life Sciences（サントリー生命科学財団）
Takeda Science Foundation（武田科学振興財団）
Terumo Life Science Foundation（テルモ生命科学振興財団）
The Cell Science Research Foundation（細胞科学研究財団）
The Chemo-Sero-Therapeutic Research Institute（化学及血清療法研究所）
The Former Tokyo Biochemical Research Foundation (TBRF)（東京生化学研究会）
The Higo Foundation for Promotion of Medical Education and Research（肥後医育振興会）
The Ichiro Kanehara Foundation（金原一郎記念医学医療振興財団）
The Inamori Foundation（稲森記念財団）
The Japan Prize Foundation（国際科学技術財団）
The Japan Research Foundation for Healthy Aging（健康加齢医学振興財団）
The Japan Science Society (JSS)（日本科学協会）
The Japan Society for the Promotion of Science (JSPS)（日本学術振興会）
The Mitsubishi Foundation（三菱財団）
The Naito Science & Engineering Foundation（内藤記念科学振興財団）
The Nakatomi Foundation（中冨健康科学振興財団）
The NOVARTIS Foundation (Japan) for the Promotion of Science（ノバルティス科学振興財団）
The Sumitomo Foundation（住友財団）
The Waksman Foundation of Japan Inc（日本ワックスマン財団）
The Yasuda Medical Foundation（安田記念医学財団）
Uehara Memorial Foundation（上原記念生命科学財団）
Yamada Bee Farm（株式会社山田養蜂場）― 76 ―

8. Appendix

8-6. List of Seminars & Symposia • Symposia

1.

IRCMS Kickoff Symposium, 2015/1/15, Hideo Baba, Kumamoto
University; Charles Bangham, Imperial College London; Atsushi
Iwama, Chiba University; Gou Young Koh, Kumamoto University;
Richard Koup, National Institutes of Health (NIH); Ben D.
MacArthur, University of Southampton; Masao Matsuoka, Kyoto
University; Andrew McMichael, University of Oxford; Ryuichi
Nishinakamura, Kumamoto University; Shinya Suzu, Kumamoto
University; Hitoshi Takizawa, Kumamoto University; Daniel G.
Tenen, National University of Singapore, Onsite, Kumamoto City

2.

International Symposium on Nuclear Structure andEpigenetics, 2015/8/27, Kerstin Bystricky, University of Toulouse;
ShinjiroHino, Kumamoto University; Yorifumi Satou, Kumamoto
University; Kannanganattu V. Prasanth, University of Illinois; Shinichi
Nakagawa, RIKEN; TokioTani, Kumamoto University; Noriko Saitoh,
Kumamoto University, Onsite, Kumamoto City3.

The 2nd Kumamoto IRCMS International Symposium, ,2016/11/2, Tsvee Lapidot, Weizmann Institute of Science; Linheng
Li, Stowers Institute for Medical Research; Hitoshi Takiawa,
Kumamoto University; Yorifumi Sato, Kumamoto University;
Hitoshi Niwa, Kumamoto University; Koichi Nishiyama, Kumamoto
University; Takamasa Ueno, Kumamoto University; Pilhan Kim,
KAIST; Gang Huang, Cincinnati Children’s Hospital Medical Center;
Richard Koup, National Institutes of Health (NIH); Andres Finzi,
Université de Montréal; Francesco Simonetti, University of Milan;
Tetsuro Matano, National Institute of Infection Disease(NIID),
Kumamoto University; Masaya Baba, Kumamoto University;
Shinya Suzu, Kumamoto University; Takatsugu Ishimoto,Kumamoto University; Hiroyuki Gatanaga, National Center for
Global Health and Medicine(NCGM), Kumamoto University; RafaelCasellas, National Institutes of Health (NIH)RIKEN; Kenji Maeda,
National Center for Global Health and Medicine(NCGM); Yosuke
Mukoyama, National Institutes of Health (NIH); Taketoshi Mizutani,
Institute of Microbial Chemistry(IMC); Ryuichi Nishinakamura,
Kumamoto University; Guojun Sheng, Kumamoto University;
Zhansong Lin, Kumamoto University; Atsuko Hachiya, Nagoya
Medical Center; Kenzo Tokunaga, National Institute of Infection Disease(NIID), Onsite, Kumamoto City

4.

IRCMS mini-symposium “Cellular Cross-talk in Health andDisease”, 2017/3/2, Angela Nieto, Instituto de Neurociencias
CSIC-UMH; Masaya Baba, Kumamoto University; Guojun Sheng,
Kumamoto University; Toshio Suda, Kumamoto University;
Tomomasa Yokomizo, Kumamoto University; Takatsugu Ishimoto,Kumamoto University, Onsite, Kumamoto City5.

The 29th IRCMS Seminar (IRCMS mini-symposium “Cell Fatein Development”),, 2017/9/21, Tsvee Lapidot, Weizmann Instituteof Science; Fumio Matsuzaki, RIKEN; Shosei Yoshida, National
Institute for Basic Biology (NIBB); Tatsuo Shibata, RIKEN; Toshihiko
Fujimori, National Institute for Basic Biology (NIBB); Kohei Homma,
Keio University, Onsite, Kumamoto City

6.

The 1st KU-KAIST Joint Symposium (The 3rd IRCMS &IROAST Joint Seminar), 2018/10/21-2018/10/23, Hitoshi Takiawa,
Kumamoto University; Jinjyu Han, KAIST; Goro Sashida, Kumamoto
University; Koichi Nishiyama, Kumamoto University; Injune
Kim, KAIST; Guojun Sheng, Kumamoto University; Ho Min Kim,
KAIST; Takumi Higaki, Kumamoto University; Hidenobu Mizuno,
Kumamoto University; Pilhan Kim, KAIST; Aeju Lee, Kumamoto
University; Young Seok Ju, KAIST; Yorifumi Sato, Kumamoto
University; Yufeng Zheng, Kumamoto University; Jaemyoung Suh,
KAIST, Onsite, Daejeon Metropolitan City, Korea

7.

The 2nd KU-KAIST Joint Symposium (The 4th IRCMS &IROAST Joint Seminar), 2019/01/23-2019/01/25, Young Seok
Ju, KAIST; Hiroto Ohguchi, Kumamoto University; Tokio Tani,
Kumamoto University; Takashi Minami, Kumamoto University; Jae
Myoung Suh, KAIST; Takatsugu Ishimoto, Kumamoto University;
Su-Hyung Park, KAIST; Masaya Baba, Kumamoto University; Injune
Kim, KAIST; Yuichiro Arima, Kumamoto University; Gou Young
Koh, KAIST; Toshio Suda, Kumamoto University; Takuro Niidome,
Kumamoto University; Thinh Minh Do, Kumamoto University; Pilhan
Kim, KAIST; Yoshitaka Nakanishi, Kumamoto University, Onsite,
Kumamoto City and Aso City

8.

JSPS & NUS 2nd Joint Symposium “New Horizonz in Normal
and Cancer Stem Cell Research”, 2018/1/18-2018/1/20, Gou
Young Koh, KAIST; César Nombela-Arrieta, University of Zurich;
Wee Joo CHNG, Cancer Science Institute of Singapore; Yoshiaki
Ito, Cancer Science Institute of Singapore; Sudhakar JHA, Cancer
Science Institute of Singapore; H. Phillip KOEFFLER, Cancer
Science Institute of Singapore, University of California Los Angeles;
Nam Long NGUYEN, Cancer Science Institute of Singapore;
Takaomi SANDA, Cancer Science Institute of Singapore; Patrick
TAN, Cancer Science Institute of Singapore; Bin Tean TEH, Cancer
Science Institute of Singapore; Daniel G. TENEN, Cancer Science
Institute of Singapore; Fumio ARAI, Kyushu University; Masaya
BABA, Kumamoto Univerity; Patrick Tan, National University ofSingapore; Susumu GOYAMA, University of Tokyo; Atsushi HIRAO,
Kanazawa University; Takatsugu Ishimoto, Kumamoto University;
Hiroaki HONDA, Tokyo Women’s Medical UniversityAgency for
Science, Technology and Research (A*STAR); Atsushi Iwama,
Chiba University; Yoshikane Kikushige, Kyushu University; David
Epstein, Duke-NUS; Fan-Yan Wei, Kumamoto University; Kazuhiro
MORISHITA, Miyazaki University; Masao Matsuoka, Kumamoto
University; Darren Qiancheng Tan, National University ofSingapore; Polly Leilei Chen, National University of Singapore; Emi
NISHIMURA, Tokyo Medical and Dental University; Motomi OSATO,
Kumamoto University; Md. Zakir Hossain, National University ofSingapore; Masashi SANADA, Nagoya Medical Center; Toshiro
SATO, Keio University; Toshio SUDA, Kumamoto University,
National University of Singapore; Hitoshi TAKIZAWA, Kumamoto
University; Keiyo TAKUBO, National Center for Global Health andMedicine, Onsite, Kumamoto City

― 77 ―

9.

The 35th International Kumamoto Medical Bioscience
Symposium, 2019/11/11, Gerhard Christofori, University of Basel;
Jonas Fuxe, Karolinska Institute; Amy Wilson, Hudson Institute ofMedical Research; Koshi Mimori, Kyushu University; Rik Derynck,
University of California San Francisco; Takanori Eguchi, Okayama
University; Masatoshi Takeichi, RIKEN; Andrew Ewald, Johns
Hopkins University; Xing Dai, University of California Irvine; PierreFrançois Lenne, Marseille Developmental Biology Institute (IBDM);David Sherwood, Duke University; Vignesh Sundararajan, National
University of Singapore; Naoto Ueno, National Institute for Basic
Biology (NIBB); Yoshiko Takahashi, Kyoto University; Ryuichi
Nishinakamura, Kumamoto University; Martin Cheung, Universityof Hong Kong; Robert Mayor, University College London;
keith Mostov, University of California San FranciscoKumamoto
University; Pilar Blancafort, University of Western Australia; Klaudia
Skrzypek, Jagiellonian University; Andreas Möller, QIMR-B; Katia
Georgopoulos, Harvard University; Dingcheng Gao, Cornell
University; Cedric Blanpain, Université libre de Bruxelles; Qing
Nie, University of California Irvine; Mohit Jolly, Indian Institute of
Science; Ruby Huang, National Taiwan University; Julia Kleemann,
University of Erlangen-Nuremberg; Kazuhide Watanabe, RIKEN;
Thomas Brabletz, University of Erlangen-Nuremberg; Hideyuki
Saya, Keio University; Yibin Kang, Princeton University; Nami
Sugiyama, University of Basel; Seong-jin Kim, Seoul National
University; Jean-Paul Thiery, National University of Singapore;
Ritu Shrestha, University of Queensland; Hiroshi Sasaki, Osaka
University; Christoph Viebahn, Göttingen; Yuki Sato, Kyushu
University; Angela Nieto, Instituto de Neurociencias UMH-CSIC; Ian
Overton, Queen’s University Belfast; Sofiane Hamidi, Kumamoto
University; Kyra Campbell, University of Sheffield; Anna-Katherina
Hadjantonakis, Memorial Sloan Kettering Cancer Center; FuchouTang, Peking University; Yukiko Nakaya, RIKEN; Barry Thompson,
Crick Institute; Theresa Vincent, Uppsala University; Kathryn
Cheah, University of Hong Kong, Onsite, Kumamoto City

10. KU-KAIST Joint Symposium / The 6th IROAST/IRCMS
Joint Seminar, 2019/11/11- 2019/11/12, Young Seok Ju, KAIST;
Takatsugu Ishimoto, Kumamoto University; Inkyung Jung, KAIST;
Ruda Lee, Kumamoto University; Terumasa Umemoto, Kumamoto
University; You-Me Kim, KAIST; Yoshikazu Hayashi, Kumamoto
University; Aiko Sada, Kumamoto University; Jinju Han, KAIST;
Hidenobu Mizuno, Kumamoto University; Ki-Jun Yoon, KAIST;
Jae-Byum Chang, KAIST; Taeyun Ku, KAIST; Koichi Nishiyama,
Kumamoto University; Jae Myung Suh, KAIST; Yuichiro Arima,
Kumamoto University; Jee Myung Yang, KAIST; Tomomasa
Yokomizo, Kumamoto UniversityKAIST; Sanshiro Hanada,
Kumamoto University; Jingu Lee, KAIST; Minwoo Kim, Kumamoto
University; Kwang-eun Kim, KAIST, Onsite, Daejeon Metropolitan
City, Korea
11. The 7th IROAST-IRCMS Joint Seminar, 2020/8/25, Hidenobu
Mizuno, Kumamoto University; Koichi Nishiyama, Kumamoto
University; Tokio Tani, Kumamoto University; Buluke, Kumamoto
University; Sheng Guojun, Kumamoto University; Kazuki
Takashima, Kumamoto University; Makiko Kobayashi, Kumamoto
University, Online, 12. The 5th IRCMS Symposium JSPS Core to Core Program
Virtual Mini Symposium, 2020/11/11, Terumasa Umemoto,Kumamoto University; Marie-Dominique Filippi, Cincinnati Children’s
Hospital Medical Center; Goro Sashida, Kumamoto University;
Gang Huang, Cincinnati Children’s Hospital Medical Center, Online,-

13. The 4th KAIST-KU Workshop and Joint Symposium ,2021/2/22, Akiko Kobayashi, Kumamoto University; Jessie
Sungyun Jeon, KAIST; Shinichiro Sawa, Kumamoto University;
Daisuke Kurotaki, Kumamoto University; Jong-Eun Park, KAIST;
Tokio Tani, Kumamoto University; Jinkuk Kim, KAIST; Pilhan Kim,
KAIST; Hidenobu Mizuno, Kumamoto University; Won-Il JEONG,
KAIST; Masashi Muramatsu, Kumamoto University; Ji Eun OH,
KAIST; Keiichiro Ishiguro, Kumamoto University; Takuro NIIDOME,
Kumamoto University; Hyun Jung CHUNG, KAIST; Yufeng ZHENG,
Kumamoto University; 0, 0; 0, 00; Aileen Rowan, Imperial College
London; Jun-ichirou Yasunaga, Kumamoto University; Yorifumi
Satou, Kumamoto University; Charles Bangham, Imperial College
London, Online, 14. The 6th IRCMS Symposium Core to Core Program Virtual
Mini Symposium on HTLV-1 Leukemogenesis, 2021/3/12,
Aileen Rowan, Imperial College London; Jun-ichirou Yasunaga,
Kumamoto University; Yorifumi Satou, Kumamoto University;
Charles Bangham, Imperial College London, Online, 15. The 7th IRCMS Symposium Core to Core Program Virtual
Mini Symposium on Immunity, 2021/5/27, Mikael Sigvardsson,
Lund University; Marc H.G.P. Raaijmakers, Erasmus MC, The
Netherlands; Filipe Pereira, Lund University; Daisuke Kurotaki,
Kumamoto University, Online, 16. The 8th IRCMS Symposium 74th IRCMS seminar mini
Symposium, 2021/11/4, Dr. Koji Eto, Kyoto University; Naoya
Takayama, Chiba University; Satoshi Yamazaki, University of Tsukuba, Onsite and Online, Kumamoto City
17. The 9th IRCMS Symposium Core to core Program Symposium
on Hematopoiesis and Leukemia, 2022/1/13-2022/1/14, Ayako
Ishizu, Tokyo Women’s Medical University; Fumio Arai, Kyushu
University; Hitoshi Takizawa, Kumamoto University; Atsushi Hirao,
Kanazawa University; Kenichi Miharada, Kumamoto University;
Takaomi Sanda, National University of Singapore; Tomohiko
Tamura, Yokohama City University; Toshio Suda, Kumamoto
University; Jonas Larsson, Lund University; Elisa Laurenti,
University of Cambridge; Mamiko Sakata-Yanagimoto, University ofTsukuba; Goro Sashida, Kumamoto University; Akihiko Yokoyama,
National Cancer Center; Atsushi Iwama, University of Tokyo; Issay
Kitabayashi, National Cancer Center; Takuro Nakamura, Cancer
Institute of JFCR; Toshio Kitamura, University of Tokyo,Onsite andOnline, Kumamoto City
18. The 10th IRCMS Symposium 78th IRCMS seminar mini
Symposium, 2022/3/15, Nobuaki Takahashi, Kyoto University; Joji
Kusuyama, Tohoku University, Onsite and Online, Kumamoto City
19. The 11th IRCMS Symposium Core to Core Symposium,
2022/6/14, Pedro P. Rocha, National Institutes of Health (NIH);
Leah Rosin, National Institutes of Health (NIH); Azusa Inoue, RIK
EN, Online, 20. The 12th IRCMS Symposium
83rd IRCMS seminarsymposium, 2022/7/29, Rohit Pappu, Washington University;
Masayuki Oginuma, Osaka University; Yusuke Ono, Kumamoto
University; Jin Zhang, University of California San Diego; Shosei
Yoshida, National Institute for Basic Biology (NIBB); Oleg Gusev,
Juntendo University; Daisuke Nanba, University of Tokyo, Onsite
and Online, Kumamoto City

― 78 ―

21. The 5th KU-KAIST Joint Symposium, 2022/10/20-2022/10/21,
Yuichiro Arima, Kumamoto University; Won-il Jeong, KAIST; Pilhan
Kim, KAIST; Keisuke Ito, Albert Einstein College of Medicine;
Jinju Han, KAIST; Hidenobu Mizuno, Kumamoto University; Hyun
Jung Chung, KAIST; Ji Min Lee, KAIST; Yoon Sung Nam, KAIST;
Daisuke Kurotaki, Kumamoto University; Sungmin Son, KAIST;
Hitoshi Takizawa, Kumamoto University; Sho Kubota, Kumamoto
University; Ji Eun Oh, KAIST; Tatsuya Morishima, Kumamoto
University; Ji Ho Park, KAIST; Sangyong Jon, KAIST, Onsite andOnline, Kumamoto City
22. The 13th IRCMS Symposium
Applications of Mass
Cytometry in Basic and Clinical Research, 2023/1/26,
Takatsugu Ishimoto, Kumamoto University; Nagesh Kalakonda,
University of Liverpool, Online, 23. The 14th IRCMS Symposium Core to Core Symposium,
2023/2/15, Mayu Kawai, Kumamoto University; Jie Bai, Kumamoto
University; Mayumi Hirayama, Kumamoto University; Mariko Morii,
Kumamoto University; Atsuko Yonemura, Kumamoto University;
Daichi Inoue, Foundation for Biomedical Research and Innovation
at Kobe; Kenta Kikuchi, Kumamoto University; Kiyoka Saito,
Kumamoto University; Alban Johansson, Kumamoto University;
Yuxin Wang, Kumamoto University; Pui Yu Ho, Kumamoto
University; Ai Kotani, Tokai University, Onsite and Online,
Kumamoto City
24. The 15th IRCMS Symposium 90th IRCMS SeminarSymposium, 2023/2/20, Daisuke Umeno, Waseda University;
Hina Kosakamoto, RIKEN; Hiroaki Okae, Kumamoto University;
Hiroko Sano, Kurume University; Jacob Hanna, Weizmann Instituteof Science; Julia Marzi, University of Tübingen; Kenichi Miharada,
Kumamoto University; Li-Kun Phng, RIKEN; Reika Tei, Stanford
University; Yingke Wu, Max Planck Institute for Polymer Research,
Onsite and Online, Kumamoto City

29. The 38th International Kumamoto Medical and Bioscience
Symposium “Construction of Functional Nervous System”,
2023/12/14-2023/12/15, Hayato Chiba, Tohoku University; Natalia
De Marco Gracia, Weill Cornell Medicine; Takashi Hayakawa,
Nihon University; Yusuke Hirabayashi, University of Tokyo; Takeshi
Imai, Kyushu University; Kazuya Iwamoto, Kumamoto University;
Partrick Kanold, Johns Hopkins University; Chiaki Maruyama,
Tokyo Metropolitan Institute of Medical Science; Goichi Miyoshi,
Gunma University; Hidenobu Mizuno, Kumamoto University;
Hirofumi Morishita, Mount Sinai Medicine; Naoki Nakagawa,National Institute of Genetics; Tatsuo Sato, Kagoshima University;
Tomohisa Toda, Friedrich-Alexander-Universität; Chitoku Toda,
Kumamoto University; Naofumi Uesaka, Tokyo Medical and
Dental University; Jun Hatakeyama, Kumamoto University, Onsite,
Kumamoto City
30 The 21st IRCMS Symposium on The Dynamics andDevelopment of Normal and Malignant Hematopoietic StemCells, 2024/2/20-2024/2/21, Takayuki Hoshii, Chiba University;
Hannah Uckelmann, Goethe University Frankfurt; Toshio Suda,
Kumamoto University; Satoshi Yamazaki, University of Tokyo;
Atsushi Iwama, University of Tokyo; Satoru Miyagi, Shimane
University; Goro Sashida, Kumamoto University; Nariko Arimura,Tohoku University; Makoto Makishima, Nihon University; Kenichi
Miharada, Kumamoto University; Yuichiro Arima, Kumamoto
University; Yorifumi Sato, Kumamoto University; Junichiro
Yasaunaga, Kumamoto University; Hitoshi Takizawa, Kumamoto
University; Takanori Teshima, Hokkaido University; Onsite andOnline, Kumamoto CityCity and Aso City

25. The 16th IRCMS Symposium Korea-Japan Blood and Vessel
Symposium in Kumamoto, 2023/7/11, Kyungsun Heo, Chungnam
National University; Junyeop Lee, Asan Medical Center, University
of Ulsan; Norika Liu, Jikei University; Akiyasu Iwase, The Universityof Tokyo; Atsushi Hirao, Kanazawa University, Onsite and Online,
Kumamoto City
26. The 17th IRCMS Symposium Rise of Diversity in Sciences,2023/09/28-2023/9/29, Mahekta Gujar, National University ofSingapore; Momoko Hamano, Kyushu Institute of Technology;
Mizuki Honda, Kyoto University; Mai Inagaki, Tokushima Univerity;
Yasuko Inaba, Nara Institute of Science and Technology (NAIST);
Ayaka Ito, Nagoya University; Sayako Katada, Kyushu University;
Tatsuyoshi Kono, Indiana University; Miho Sekai, Kyoto University;
Guojun Sheng, Kumamoto University; Miyuki Suzuki, California
Institute of Technology; Miwa Tanaka, Japanese Foundation For
Cancer Research; Kanako Wakahashi, Fundación Centro Nacionalde Investigaciones Cardiovasculares (CNIC); Yingzi Yang, Harvard
University, Onsite and Online, Kumamoto City
27. The 18th IRCMS Symposium 26th IRCMS symposium,
2023/10/24, Emmanuelle Passegué, Columbia University; Amélie
Collins, Columbia University; Daisuke Kurotaki, Kumamoto
University; Kenichi Miharada, Kumamoto University; Hitoshi
Takizawa, Kumamoto University, Onsite and Online, Kumamoto
City
28. The 6th KU-KAIST Symposium, 2023/11/6, Young Seok Ju,
KAIST; Rieko Asai, Kumamoto University; Woojung Shin, KAIST;
Yuichiro Arima, Kumamoto University; Jae Myung Suh, KAIST;
Hyeuk Jong Lee, Institute for Basic Science (IBS); Jinkuk Kim,
KAIST; Daisuke Kurotaki, Kumamoto University; Hitoshi Takizawa,
Kumamoto University; Ki-Hun Jeong, KAIST; Pilhan Kim, KAIST;
Seon Pyo Hong, Institute for Basic Science (IBS), Onsite, Daejeon
Metropolitan City, Korea― 79 ―

8. Appendix
1.

8-6. List of Seminars & Symposia • IRCMS Seminars

The inaugural IRCMS seminar, 2014/6/2, Ng King Pan,
National University of Singapore, Chronic Myelogenous
Leukemia:resistance, persistence, and beyond; Motomi
Osato, National University of Singapore, Runx1 hematopoietic
stem cell enhancer and its activity in other organs; Fumio
Arai, Kyushu University, Paired daughter cell analyses for the
regulation of the self-renewal division of hematopoietic stem
cells; Onsite2.

The 2nd IRCMS Seminar, 2014/7/9, Toshio Kuroki, Japan
Society for the Promotion of Science, The key to create theworld-acknowledged institute - from the experience of World
Premier International Research Center Initiative (WPI); Onsite

3.

The 1st Ad Hoc IRCMS Seminar, 2014/7/31, Ryuichi
Nishinakamura and Atsuhiro Taguchi, Kumamoto University,
Redefining the origin of nephron progenitors enables
generation of three-dimensional kidney structures frompluripotent stem cells.; Masaya Baba, Kumamoto University,
Functional role of a novel tumor suppressor gene Flcn inkidney; Motoko Yanagita, Kyoto University, New concept andcontroversies in renal fibrosis; Onsite

4.

The 3rd IRCMS Seminar, 2014/9/3, Yosuke Mukoyama,
National Institute of Health (NIH), Studles on Neuro-Vascular
Wlring Develop Scientific Networking for Career Development~Get Tenure at NIH?~; Onsite

5.

6.

The 2nd Ad Hoc IRCMS Seminar, 2014/10/27, Akiyoshi
Uemura, Nagoya city University, Novel drug targets for thetreatment of diabetic retinopathy; Yoshiaki Kubota, Keio
university, Neuro-vascular interaction in retinal development
and pathology; Sumiko Watanabe, University of Tokyo, Cell
Lineage specific mechanisms of retinal cell differentiation andmaintenance by histone lysine residues methylation; Onsite
International Collaborative Research Group for Life Sciences,
2014/11/19, Alvaro Köhn-Luque, Dresden University ofTechnology, Mathematical and computational modeling inbiomedical research: What is it good for H; Onsite

7.

International Collaborative Research Group for Life Sciences,
2014/12/16, Leonid L Nikitenko, Keble College, University of
Oxford, Novel (lymph)angiogenic factors and their receptors:
From discovery towards translational research?; Onsite

8.

The 3rd Ad Hoc IRCMS Seminar, 2014/12/17, Jun Yokota,
Institute of Predictive and Personalized Medicine of Cancer(IMPPC), Career Genome: From Knowledge to Cancer Clinics;
Onsite9.

Cancer stem-like cells in liver-fluke associatedcholangiocarcinoma, 2015/1/9, Kulthida Vaeteewoottacharn,
Khon Kaen University, Cancer stem-like cells in liver-fluke
associated cholangiocarcinoma; Onsite

10. IRCMS & CAIDS Seminar, 2015/1/23, Reuben S. Harris,
University of Minnesota, Virus Restriction and Cancer Mutationby the APOBEC Family of DNA Cytosine Deaminases; Onsite
11. The 4th Ad Hoc IRCMS Seminar, 2015/1/28, Takahiro Maeda,
Brigham and Women’s Hospital, Harvard Medical School, Roleof the transcription factor LRF in normal hematopoiesis andfetal globin repression.; Onsite
12. The 5th Ad Hoc IRCMS Seminar, 2015/5/18, Ng King Pan,
National University of Singapore, Role of HIFs & ARG2 inhematological malignancy; Yosuke Mukoyama, National
Institutes of Health (NIH), Guidance of Vascular Patterning in
Organ Development; Onsite

13. The 5th IRCMS Seminar, 2015/6/11, Guojun Sheng, RIKEN,
Using the avian model for understanding human diseases;
Onsite
14. The 6th Ad Hoc IRCMS Seminar, 2015/6/12, Momoko
Yoshimoto, Indiana University, Layered immune theory-Stemcell independent lymphopoiesis in the mouse embryo; Onsite
15. Retro - Kyoten & IRCMS International Seminar, 2015/6/26,
Marcelo J. Kuroda, Tulane National Primate Research Center,
Evidence that long-lived macrophages are an important SIV
reservoir in the rhesus macaque model of AIDS: lessons fromthe lung; Onsite
16. The 7th IRCMS Seminar, 2015/7/13, Atsushi Iwama, Chiba
University, Epigenetic regulation of hematopoiesis by thepolycomb-group genes; Nobuyuki Takakura, Osaka University,
Discovery of endothelial stem cell population in the preexisting blood vessels; Toshio Suda, Kumamoto University,
Fate of hematopoietic stem cells; Kiyoshi Takatsu, Toyama
Prefectural Institute for Pharmaceutical Research, Revisiting
the identification of IL-5 and IL-5R: IL-5-producing innate
lymphoid cell and its role in the immunity and inflammation;
Onsite17. The 8th IRCMS Seminar, 2015/7/14, Tomas Hanke, Universityof Oxford, Development of an HIV-1 Vaccine; Onsite
18. The 9th IRCMS Seminar, 2015/9/24, Tsvee Lapidot, Weizmann
Institute of Science, Metabolic regulation of blood and bone
forming stem cells: The role of ROS and Nitric Oxide; Onsite
19. The 10th Ad Hoc IRCMS Seminar, 2016/1/21, Koji Eto, Kyoto
University, Engineering biogenesis of human platelets by anewtype of bioreactor system; Seishi Ogawa, Kyoto University,
Clonal origin of Myelodysplastic syndromes; Onsite
20. The 11th Ad Hoc IRCMS Seminar, 2016/3/10, Mikael
Sigvardsson, Lund University, Shaping up a lineage: Lessons
from B-Lymphocyte development; Onsite
21. Seminar by International collaborative Research Group forLife Sciences （生命科学系国際共同研究拠点共同研究旅費
支 援 事 業 セ ミ ナ ー）, 2016/3/10, Dong Ha Bhang, Universityof Pennsylvania, Restoration of spermatogenesis after
chemotherapy-induced infertility; Federica Bertocchini,
IBBTEC, ?; Onsite
22. The 12th Ad Hoc IRCMS Seminar, 2016/3/17, Charles R M
Bangham, Imperial College London, How does HTLV-1 persist
and cause disease?; Onsite
23. The 13th IRCMS Seminar, 2016/4/1, Mary Carrington, National
Institutes of Health (NIH), Immunogenetic variation that
impacts human health and disease: a tricky balancing act;
Onsite24. The 14th IRCMS Seminar, 2016/4/1, Sarah L Rowland-Jones,
University of Oxford, New insights into protective immunity ininfant HIV-1 and adult HIV-2 infection; Onsite
25. The 15th IRCMS Seminar, 2016/6/30, Marcelo J. Kuroda,
Tulane National Primate Research Center, Macrophagedamage by SIV promotes TB reactivation in the TB/SIV rhesus
co-infection model; Onsite
26. The 16th IRCMS Seminar Towards a theory of stem celldifferentiation, 2016/7/27, Ben D Macarthur, University ofSouthampton, Towards a theory of stem cell differentiation;
Onsite― 80 ―

27. The 17th IRCMS Seminar, 2016/8/10, Victor Appay, Institut
national de la santé et de la recherche médicale (INSERM),
Decline of immunity with aging in humans; Sarah RowlandJones, University of Oxford, The role of host restriction factors
from the TRIM family in protective immunity in HIV-2 infection;
Onsite28. International Collaborative Research Group for Life Sciences
seminar, 2016/10/28, Elisa Laurenti, University of Cambridge,
Molecular and functional heterogeneity in the humanhaematopoietic stem cell compartment; Onsite
29. The 18th IRCMS Seminar, 2017/1/26, Masahiro Fukuda,
National University of Singapore, In vivo imaging of
intraperitoneal organs using two-photon microscope; Onsite
30. The 19th IRCMS Seminar, 2017/2/2, Christian Schönbach,Kumamoto University, Microbiome parameters at theintersection of environment and human health; Guojun Sheng,
Kumamoto University, Understanding disease mechanism
using developmental models of epithelial-mesenchymaltransition; Goro Sashida, Kumamoto University, Two facesof the polycomb-group gene EZH2 in hematologicalmalignancies; Hitoshi Takizawa, Kumamoto University,
Hematopoietic stem cell behavior in homeostasis andinflammation; Onsite
31. The 21st IRCMS Seminar, 2017/3/16, Dmitriy Viderman,
Nazarbayev University, Potential Benefits of Hemofiltration in
Acute Ischemic Stroke Management; Onsite
32. The 22nd IRCMS Seminar, 2017/3/22, Paul Horton, National
Institute of Advanced Industrial Science and Technology(AIST), Hallmarks of slow translation initiation revealed in
mitochondrially localizing mRNA sequences; Onsite
33. The 23rd IRCMS Seminar, 2017/4/20, Yosuke Tanaka,
University of Tokyo, Resolving early mesoderm diversification
through single-cell expression profiling; Aeju Lee, Kumamoto
University, Polymeric Nanoconstructs for multimodal imaging
and combination therapy; Onsite
34. The 24th IRCMS Seminar, 2017/6/7, Tomohiko Tamura,
Yokohama City University, Regulation of myelopoiesis by thetranscription factor IRF8; Onsite
35. The 25th IRCMS Seminar, 2017/6/9, Eishu Hirata, Kanazawa
Medical University, Imaging ‘Failure’ How BRAF inhibition
generates drug tolerant microenvironments; Onsite
36. The 26th IRCMS Seminar, 2017/6/12, Estelle DUPREZ, Centreof Research in Cancerology of Marseille, Epigenetic regulation
in myeloid malignancies: Molecular mechanism and clinicalutility.; Onsite
37. The 27th IRCMS Seminar, 2017/6/27, Masahiro Ono, Imperial
College London, Investigating in vivo dynamics of regulatory
T-cell generation by novel transcriptional timer; Onsite
38. The 28th IRCMS Seminar, 2017/9/11, Makoto Yawata, National
University of Singapore, Human diversity in the regulatory
mechanisms of natural killer cells - implications for immunology
and clinical medicine; Onsite

39. The 30th IRCMS Seminar, 2017/10/23, Keisuke Ito, Albert
Einstein College of Medicine, Metabolism, control of cell fatedecisions, and hematopoietic stem cell renewal; Momoko
Yoshimoto, University of Texas, Hematopoietic stem cell andstem cell independent hematopoiesis; Elisa Laurenti, Universityof Cambridge, Functional and molecular heterogeneity within
the human haematopoietic stem cell compartment; Daisuke
Nakada, Baylor College of Medicine, Oncogenic Prdm16
mutation transforms MEPs to cause myeloid malignancies;
Onsite40. The 31st IRCMS Seminar, 2017/11/15, Kasem Kulkeaw,
Kyushu University, Study of a new player in epigenetic
regulation of erythropoiesis; Onsite
41. The 32nd IRCMS Seminar, 2017/12/20, Noriko Satake,
University of California Davis, Beyond chemotherapy :Targeting cancer and cancer stem cells; Onsite
43. The 33rd IRCMS Seminar, 2018/2/27, Vladimir Brusic,
Nazarbayev University, Machine Learning and Artificial
Intelligence in Biomedicine; Onsite
42. IRCMS & IROAST Joint Seminar on Bioengineering |Cutting
Edge Collaborations, 2018/1/22, Koichi Nishiyama, Kumamoto
University, interdisciplinary approaches to understanding of
the underlying mechanisms of anglogenesis; Yorifumi Sato,
Kumamoto University, Application of next generation DNA
sequecing technology for viral research; Yufeng Zheng,
Kumamoto University, Metallic Biomaterials used in medicine;
Aeju Lee, Kumamoto University, Multifunctional nanoparticles
for imaging and therapy; Shinya Hayami, Kumamoto
University, Multi-function materials-Aiming for collaboration
between medicine and engineering-; Toshifumi Mukunoki,
Kumamoto University, Application of micro-focused X-ray
CT scanner for geoenvironmental engineering; Toshitaka
Yamakawa, Kumamoto University, Wearable and implantablesensor electronics for health and clinical care; Gou Koutaki,
Kumamoto University, Image processing through pharmacyengineering collaboration; Yuta Nakashima, Kumamoto
University, Microdevices and micro-nano technology for biomedical application; Hayato Ishikawa, Kumamoto University,
Isolation, total synthesis and medimedicinal chemistry of
biologically active natural product; Onsite
44. The 34th IRCMS Seminar, 2018/3/19, Jin-Sung Park, Institutefor Basic Science (IBS), Tumor Vessel Normalization : An
Alternative Approach for Treating Cancer; Onsite
45. The 35th IRCMS Seminar, 2018/3/20, Yoshikane Kikushige,
Kyushu University, Identification of BCAAs metabolism
pathway as a common machinery for maintaining thestemness of human acute leukemia; Anthony D. Ho, University
of Heidelberg, Proteome Landscapes of Aging of Human
Hematopoietic Stem Cells & Warburg Effect; Onsite
46. The 36th IRCMS Seminar, 2018/4/3, Tatsuya Morishima,
University Hospital Tübingen, The role of NAMPT-NAD+-SIRTs
pathway - mediated protein deacetylation in hematopoiesis
and leukemogenesis; Onsite
47. The 37th IRCMS Seminar, 2018/4/23, Koichi Takahashi,
University of Texas MD Anderson Cancer Center, Evolution of
AML genome and epigenome during therapy; Onsite
48. The 38th IRCMS Seminar, 2018/5/31, Kenichi Miharada, Lund
University, Proteostatic control in stem cell regulation anddevelopment; Onsite― 81 ―

49. The 39th IRCMS Seminar, 2018/6/26, Paul S. Frenette, Albert
Einstein College of Medicine, Young, Old, and revitalized HSC
niches; Onsite
50. The 40th IRCMS Seminar, 2018/7/17, Takayuki Hoshii,
Harvard Medical School, Identification and targeting of thetranscriptional vulnerabilities in leukemia; Onsite
51. The 2nd IROAST & IRCMS Joint Seminar - The 33rd IROAST
Seminar, 2018/8/21, Hitoshi Takizawa, Kumamoto University,
Single cell analysis for hematopoietic stem cell biology;
Hidenobu Mizuno, Kumamoto University, In vivo two-photon
imaging to elucidate mechanisms for brain development;
Mitsuhiro Aida, Kumamoto University, Shoot stem cell formationin plant development; Takumi Higaki, Kumamoto University,
Quantification and classification of bioimages; Onsite
52. The 41st IRCMS Seminar, 2018/9/25, Hideki Makishima,
Kyoto University, Sequential acquisition of mutations inmyelodysplastic syndromes; Onsite
53. The 43rd IRCMS Seminar, 2018/10/25, Simon Mendez-Ferrer,
University of Cambridge, Microenvironmental contributions tomyeloproliferative disorders; Onsite
54. The 42nd IRCMS Seminar, 2018/11/1, Nicole Mende,
University of Cambridge, Sequential acquisition of mutations inmyelodysplastic syndromes; Onsite
55. The 44th IRCMS Seminar, 2019/1/22, Masayuki Iwasaki, Tokyo
Women’s Medical University, Essential non-canonical role for
15-PGDH in MLL-rearranged AAML stem cells; Onsite
56. The 46th IRCMS Seminar, 2019/3/26, Hisayuki Yao, Kyushu
University, Acute lymphoblastic leukemia cells make their way
to invade the central nervous system; Kentaro Hosokawa,
Kyushu University, Role of Foxp2 in the maintenance of thequiescent state of HSCs; Fumio Arai, Kyushu University,
Hematopoietic stem cell self-renewal depends on cell divisionhistory; Onsite
57. The 47th IRCMS Seminar “Hematopoietic stem cells
endowed lab” kick off seminar, 2019/4/15, Yoshikazu Hayashi,
Kumamoto University, 造 血 幹 細 胞 文 化・ 逃 走 メ カ ニ ズ ム の理解ちその制御〜治療応用を目指して〜 ; Tatsuya Morishima,
Kumamoto University, 細胞内 NAD ＋調節による造血幹細胞維持・増幅培養法の開発 ; Terumasa Umemoto, Kumamoto University,
エネルギー代謝を介した造血幹細胞維持構造 ; Kenzaburo Tani,
University of Tokyo, 遺伝子治療の歴史と最近の進歩 ; Onsite
58. The 48th IRCMS Seminar, 2019/5/24, Naihe Jing, Shanghai
Institute of Biochemistry and Cell Biology, Moleculararchitecture of lineage specification and tissue organization inearly mouse embryo; Onsite
59. The 49th IRCMS Seminar, 2019/5/28, Wooseok Seo, RIKEN,
Regulation of anti-tumor immunity by RUNX/CBF ﾀ throughfine-turning of host CCL5; Onsite
60. The 50th IRCMS Seminar, 2019/7/18, Aiko Sada, University ofTsukuba, Epithelial stem cells in tissue regeneration and aging;
Onsite61. The 51st IRCMS Seminar, 2019/7/25, Masanori Nakayama,
Max-Planck-Institute for Heart and Lung Research, Light anddark sides of aPKC in endothelial cells; Onsite

62. The 52nd IRCMS Seminar, 2019/8/27, Terumasa Umemoto,Kumamoto University, エネルギー代謝による造血幹細胞制御
構造 ; Ishimoto Takatsugu, Kumamoto University, 腫瘍間質に依存するびまん性胃がん新展メカニズムの解明 ; Arima Yuichiro,
Kumamoto University, gentle MACS を用いた新生児期心筋細胞・幹細胞単離の取り組みと応用 ; Onsite
63. The 53rd IRCMS Seminar, 2019/8/28, Shi-Hao Tan, National
University of Singapore, TAL1 Induced Core Regulatory Circuit
Regulates Expression of Downstream Oncogenic Factors
through Enhancer Associated ncRNA in T-ALL; Onsite
64. The 54th IRCMS Seminar, 2019/8/30, Baeg Gyeong Hun,
National University of Singapore, Redox homeostasis in the
Drosophila testis germline stem cell maintenance; Onsite
65. The 55th IRCMS Seminar, 2019/9/12, Adam C Wilkinson,
Stanford University, Long-Term Ex Vivo Expansion Of
Hematopoietic Stem Cells; Onsite
66. The 57th IRCMS Seminar, 2019/10/1, Koji Taniguchi, Keio
University, The role of IL-6 family cytokines in intestinal
regeneration and cancer; Onsite
67. The 56th IRCMS Seminar, 2019/10/4, Cantas Alev, Kyoto
University, Recapitulating human somitogenesis in vitro; Onsite
68. The 58th IRCMS Seminar, 2019/10/15, Marc H.G.P.
Raaijmakers, Erasmus Medical Center Cancer Institute,
Hematopoietic stem cell responses to bone marrow failure;
Onsite69. The 5th IRCMS &IROAST Joint Seminar- 60th IROAST
Seminar, 2019/10/29, Takatsugu Ishimoto, Kumamoto
University, ; Toshifumi Mukunoki, Kumamoto University, ; Jun
Otani, Kumamoto University, MicroCT-based quantification offibrosis and stiffness of tumor microenvironment; Hidenobu
Mizuno, Kumamoto University, ; Takumi Higaki, Kumamoto
University, Quantative bioimage analysis to elucidate dynamics
of stem cells in living animals; Hitoshi Takiawa, Kumamoto
University, ; Yorifumi Sato, Kumamoto University, Bioinformatics
analysis of single cell transcriptome and proteome date;
Onsite70. The 60th IRCMS Seminar, 2019/11/13, Gang Huang, Cincinnati
Children’s Hospital Medical Center, Two Drops of the Cytokine
Storm in HLH; Onsite
71. The 61st IRCMS Seminar, 2019/11/21, Nozomu Yachie,
University of Tokyo, DNA barcode technologies to dissect
heterogeneous biological systems; Onsite
72. The 62nd IRCMS Seminar, 2020/2/12, Kenichi Tanaka,
CABINET SECRETARIAT, なぜ「次世代医療基盤法」か〜現場から提供されるデータの利活用の成果が現場へ還元される社会全体の好循環を目指して〜 ; Onsite
73. The 64th IRCMS Seminar, 2020/2/13, Makiko Mochizuki, Tokyo
Women’s Medical University, Fanconi anemia protein FANCD2
confers with replication stress through the hematopoietic stem
cell expansion in fetal liver; Onsite
74. The 63rd IRCMS Seminar, 2020/2/17, Tsuneyuki Tatsuke,
Kumamoto University, 新シルク蚕業構想 - 養蚕業の過去と未来 ;Onsite75. The 66th IRCMS Seminar, 2020/7/31, Yusuke Shimakawa,
Institut Pasteur, Mother-to-child transmission of hepatitis B: canit be eliminated?; Onsite― 82 ―

76. The 7th IROAST-IRCMS Joint Seminar/ 67th IRCMS Seminar,
2020/8/25, Hidenobu Mizuno, Kumamoto University,
Quantitative bioimage analysis to elucidate dynamics ofhematopoietic stem cells in living animals; Koichi Nishiyama,
Kumamoto University, Combined in vitro and in silico analyses
to dissect roles of blood flow in metastatic intravasation ofcancer cells.; Tokio Tani, Kumamoto University, Developmentof the novel drugs for Adult T-cell Leukemia based on themodifications of nuclear morphology; Buluke, Kumamoto
University, Significance of tumor microenvironmental 15pgdh
depletion in pancreatic cancer; Guojun Sheng, Kumamoto
University, MMP sensors and sensor-based in vivo deliveryof small molecules; Kazuki Takashima, Kumamoto University,
Micro-Mechanical Characterization and Their Application
to Biomedical Sciences; Makiko Kobayashi, Kumamoto
University, Wearable low-cost ultrasonic/piezoelectric sensors
for medical applications; Online
77. The 68th IRCMS Seminar, 2020/9/2, Masahiro Ono, Imperial
College London, Mechanisms of T cell-mediated immune
regulation at the single cell level; Onsite and Online
78. The 69th IRCMS Seminar, 2020/10/20, Ayuko Hoshino, Tokyo
Institute of Technology, Cancer exosomal proteins: Roles in
pre-metastatic niche formation and biomarker potential; Online
79. The 70th IRCMS Seminar, 2020/12/25, Satoru Takahashi,
University of Tsukuba, Functional analysis of transcription
factor MafB in macrophage lineage; Onsite and Online
80. The 71st IRCMS Seminar, 2021/1/12, Masanori Nakayama,
Max-Planck-Institute for Heart and Lung Research,
Transplantation of the hiPSC-derived cardiomyocyte patch
alters extra cellular matrix composition directly affecting
cardiac fibroblasts after MI; Onsite and Online
81. The 72nd IRCMS seminar, 2021/9/13, Yoshihiro Hayashi, Tokyo
University, Dysregulated mitochondrial dynamics as a critical
mediator of MDS ineffective hematopoiesis; Online
82. The 73rd IRCMS seminar, 2021/9/29, Makoto Suematsu, Keio
University, Metabolites as molecular glues to trigger proteinprotein interactions that regulate diseases; Online
83. The 75th IRCMS seminar, 2022/1/31, Yosuke Tanaka, The
University of Tokyo, Hematopoiesis ~developmental, normal,
and leukemic~; Onsite and Online
84. The 8th IRCMS & IROAST Joint Seminar / 76th IRCMS
Seminar, 2022/3/8, Hidenobu Mizuno, Kumamoto University,
Quantitative bioimage analysis to elucidate dynamics ofhematopoietic stem cells in living animals; Guojun Sheng,
Kumamoto University, Evaluation of target-specific polymericnanoparticles for inhibitation of cancer cell metastasis; Kenichi
Miharada, Kumamoto University, A large scale red blood cell
production towards future transfusion therapies; Hiroki Matsuo,
Kumamoto University, Development of ferroelectric materials
for miniaturized energy storage applications; Online
85. The 77th IRCMS seminar, 2022/3/11, Izumi Ohigashi,
University of Tokushima, The mechanism regulating theformation of thymus function; Onsite and Online

88. The 81st IRCMS seminar, 2022/5/25, Motomi Osato, National
University of Singapore, Age matters in leukemia.; Onsite andOnline89. The 82nd IRCMS seminar, 2022/7/5, Yusuke Shimakawa,
Institut Pasteur, Development of a tool to meet local publichealth needs in resource-limited countries:a rapid diagnostic
test to identify high-risk women for hepatitis B mother-to-childtransmission; Online
90. The 84th IRCMS Seminar, 2022/8/23, Tomoya Muto,
Department of Hematology, Chiba University Hospital, Innate
immune pathways and inflammation in myeloid malignancies;
Onsite and Online
91. The 85th IRCMS seminar, 2022/9/16, Takeru Kaido, Medical
doctor and novelist, ; Masahiro Ono, Imperial College London,
感染症と北里柴三郎 ; Onsite and Online
92. The 86th IRCMS seminar, 2022/11/24, Woranop Sukparangsi,
Burapha University, Evolutionary Origin of Vertebrate OCT4/
POU5 @Functions in Supporting Pluripotency; Onsite andOnline93. The 87th IRCMS seminar, 2022/12/5, Gen-Sheng Feng,
University of California San Diego, Dissecting Shp2 and cellsignaling in health and diseases: 30 years’ research; Online
94. The 88th IRCMS seminar, 2023/1/5, Momoko Yoshimoto,
University of Texas, Multiple waves of hematopoiesis in themouse embryo constitute adult immune system; Onsite andOnline95. The 89th IRCMS seminar, 2023/1/18, Roland G. Huber,
Bioinformatics Institute, Identifying functional RNA elements inviral genomes; Onsite and Online
96. The 91st IRCMS Seminar, 2023/4/7, Ruby Huang, National
Taiwan University, Spatial Profiling of Intra-tumoral
Heterogeneity in Ovarian Clear Cell Carcinoma; Onsite andOnline97. The 93rd IRCMS Seminar, 2023/4/12, Takuya Shiota, Miyazaki
University, Photo-crosslinking Approach Reveals Mechanisms
of Protein Translocation at Work in Vivo; Onsite and Online
98. The 92nd IRCMS Seminar, 2023/4/24, Jian-Dong Huang,
University of Hong Kong, Engineering Bacteria to Form
Biological Structures and to Suppress Cancer Cell Metastasis;
Onsite and Online
99. The 94th IRCMS Seminar, 2023/5/22, Go Nagamatsu,
Yamanashi University, Analysis of the mechanism for
maintaining the dormancy of oocyte in primordial follicle using
an in vitro culture system; Onsite and Online
100. The 95th IRCMS Seminar, 2023/5/23, Anthony D Ho, University
of Heidelberg, Aging of Human Hematopoiesis is Causedby Clonal Expansion of a Subset with Senescence Signaturein the Non-primed Stem and Progenitor Cell Compartment;
Online86. The 79th IRCMS seminar, 2022/3/25, Toshimi Yoshida, Harvard
Medical School, The role of epigenetic regulators in early hem
atopoiesis; Onsite and Online

101. The 94th IRCMS Seminar, 2023/9/14, Takumi Maruhashi,
University of Tokyo, Immune regulation by LAG-3, a unique
inhibitory co-receptor; Onsite and Online

87. The 80th IRCMS seminar, 2022/4/26, Ahad Khalilnezhad,
Agency for Science Technology and Research (A*STAR),
Decoding Myelopoiesis throough the Lens of Integrative
Single-cell Multi-omics; Online

102. The 95th IRCMS Seminar, 2023/9/25, Yuanyuan Guo, Tohoku
University, Multimodal Bio-Interfaces: Advancements in
Microelectronic Fiber Technology; Onsite and Online― 83 ―

103. The 96th IRCMS Seminar, 2023/10/18, Wataru Kimura, RIEKN,
Transition in Heart Regeneration from Birth Onward; Onsite
and Online
104. The 97th IRCMS Seminar, 2023/11/10, Takashi Mikawa,
University of California San Francisco, Distance between
Homologous Chromosomes 相 同 染 色 体 間 の 距 離 の 調 節 ;Onsite and Online
105. The 98th IRCMS Seminar, 2023/11/22, Fumiko Toyoshima,
Kyoto University, Maternal Organ Remodeling and Fetal
Growth; Onsite and Online
106. 106th IRCMS Seminar, 2024/3/1, Oleg Gusev, Juntendo
University, Cartilage regeneration, quick blood coagulationand more in spiny mice Acomys cahirinus.; Dmitry Tayursky,
Kazan Federal University, Introduction of Kazan Federal
University; Onsite and Online― 84 ―

8. Appendix

8-6. List of Seminars & Symposia • KU-KAIST Seminar Series

1.

2021/7/7; Eiji Yoshihara, University of California Los Angeles,
Engineering islet organoids for immuse evasion, Online

2.

2021/7/21; Michelle Monje Deisseroth, Stanford University, Neuronglial interactions in health and desease: from cognition to cancer,
Online21. 2022/6/29; Wonhwa Cho, University of Illinois Chicago,
Development of resistance-proof cancer drugs, Online

3.

2021/7/28; Yuichiro Arima, Kumamoto University, Ketone bodymetabolism and biohomeostasis, Online

22. 2022/7/6; Ji Min Lee, KAIST, Controlling nuclear translocation of
drug machinery by epigenetic engineering, Online

4.

2021/8/11; Yusuke Ono, Kumamoto University, Hox-specified
positional memory in muscle stem cells, Online

23. 2022/7/14; Katja Schenke-Layland, University of Tubingen,
Regenerative and personalized medicine applications- Learningfrom nature’s blueprint, Online

5.

2021/8/26; Hellmut Augustin, Heidelburg University, Vascular
Control of Tunor Progression and Metastasis, Online

6.

7.

2021/9/1; Hanseul Yang, KAIST, How stem cells juggle lineage
choice: Epigenetic mechanisms of tissue regeneration of adult hairfollicle stem cell, Online
2021/9/15; Cholsoon Jang, University of California Irvine,
Organismal dietary nutrient metabolism, Online

8.

2021/9/22; Miharada Kenichi, Kumamoto University, Role for bile
acid in tissue regeneration and fetal organ development, Online

9.

2021/10/6; Jeong Seok Lee, Genome Insights, Cohort based multiomics approach to understand chronic inflammatory diseases,
Online20. 2022/6/22; Shalin Naik, Walter and Eliza Hall Institute of Medical
Research, Clonal Biology in development, immunology and cancer,
Online24. 2022/8/18; Min-Kyu Yum, Cambridge University, Tracing oncogenedriven paracrine remodelling of the intestinal stem cell niche,
Online25. 2022/8/31; Keji Zhao, National Institutes of Health (NIH), Single-celland 3D tools for epigenetic studies, Online
26. 2022/9/22; Simona Parrinello, University of College London,
Glioblastoma invasion hijacks brain development and regeneration,
Online10. 2021/10/14; Ono Masahiro, Imperial College London, Elucidation oftime-dependent changes of T-cell activities in vivo by new single
cell analysis methods, Online
11. 2021/10/28; Chujyo Takeshi, Kumamoto University, How doestransfer RNA methylation deficiency case intellectual disability?,
Online12. 2021/11/3; Pilnam Kim, KAIST, The matrix: A new dimension incancer research, Online
13. 2021/11/11; Yufeng Zheng, Peking University, Biodegradable
metals, Online
14. 2021/11/24; Mukoyama Yosuke, National Institutes of Health (NIH),
What controls UNS-specific vascular structure?, Online
15. 2022/5/11; Sagar Bapat, University of California San Fancisco,
Obesity as an altered immunological state, Online
16. 2022/5/26; Taro Kitazawa, DANDRITE-Nordic-EMBL, Epigenetic
and transcriptional basis of neuroplasticity, Online
17. 2022/6/2; Malin Parmer, Lund University, Stem cell therapy forbrain repair in Parkinson’s disease, Online
18. 2022/6/8; Hakho Lee, Harvard Medical School, Promise of liquidbiopsy for cancer management, Online
19. 2022/6/15; Chang Gon Kim, Yonsei University, Dissecting theimmune landscape of cancer for therapeutic intervention, Online― 85 ―

8. Appendix

8-6. List of Seminars & Symposia • “Science and Me” Series

1.

2021/1/8; Toshio Suda, Kumamoto University, How can we wnjoy
the research and develop the career path?, Online

20. 2022/11/4; Eiji Hara, Osaka University, Starting from curiosity,
Online2.

2021/2/12; Hitoshi Niwa, Kumamoto University, Science at thebench, Online

21. 2023/3/17; Angela Nieto, Institute of Neuroscience UMH-CSIC,
My 30 years travelling with cells from fundamental research to
Biomedicine, Online

3.

2021/3/5; Takashi Hiyama, Kumamoto University, My Research Life
for Half Century - A Long, Long Time Ago, Online

4.

2021/4/9; Aiko Sada, Kumamoto University, Decision making foryour life and career, Online

5.

2021/5/7; Gou Young Koh, KAIST, Endless Exploring toward
Vascular Research, Online

6.

2021/6/11; Yoh-suke Mukouyama, National Institutes of Health (NIH),
Connecting the dots: What makes all the difference in research,
Online7.

2021/7/2; Kazuhiro Nagata, Kyoto University, Kyoto Sangyo
University, Zig-zag pathway of my research career - Looking forthe scientific fun, Online

8.

2021/7/4; Spyros Goulas, Cell Press, My journey: from country tocountry and from the bench to an editor, Online

9.

2021/9/3; Shinichi Nishikawa, Kyoto University, Historical basis of
21st Century life science, Online

10. 2021/10/15; Hideyuki Saya, Institute for Advanced Medical
Research (IAMR) , Keio University, If you build it, they will come,Online11. 2021/11/12; Carl Zimmer, New York Times, Telling the Stories of
Science., Online
12. 2022/1/28; Kenichi Yamamura, Kumamoto University, Follow yourpassion- struggling alone without money or mouse cages, Online
13. 2022/2/25; Li-Kun Phng, RIKEN, Scientific exploration: a journeythat crosses boundaries, Online
14. 2022/3/25; Takanori Sonoda, The Maureen and Mike Mansfield
Foundation, Powered by Dreams!, Online
15. 2022/6/3; Kiyoshi Takatsu, Toyama Prefectural Institute for
Pharmaceutical Research, Molecular Basis of Innate Inflammation
and Immunometabolism, Online
16. 2022/7/1; Ryuichi Nishinakamura, Kumamoto University, Be smart.
Stay foolish, Online
17. 2022/8/19; Kinya Ota, Academia Sinica, The Straw Millionaire
Strategy: how to trade my skills, Online
18. 2022/9/2; Henry Gee, Nature, Meet a Nature editor: All you wanted
to know but were afraid to ask, Online
19. 2022/9/16; Valda Vinson, Science, Behind the scenes at Science,
Online― 86 ―

8. Appendix

8-6. List of Seminars & Symposia  • WIM (FY2022-2023)

1.

2022/4/7; Yuichiro Arima, Ketone bodies mitigate hypertension andobesity-induced myocardial hypertrophy., Online

21. 2022/10/6; Ruslan Deviatiiarov, Avian promoterome: sex
dimorphism in embryonic fibroblasts, Onsite and Online

2.

2022/4/14; Toshio Suda, Thrombopoietin as an expansion factor
for hematopoietic stem cells, Online

22. 2022/10/13; Nicole Pui-Yu Ho, Human MSC-derived bone organ
improves self-renewal of transplanted human HSCs, Onsite andOnline3.

2022/4/21; Ahmad Shah Adil Ishtiyaq, Elucidation of Mitol Functionfor Hematopoietic Stem Cell Maintenance, Online

4.

2022/4/28; Maiko Sezaki, Hematopoietic stem and progenitor cells
integrate microbial signals to promote tissue repair, Online

5.

2022/5/12; Ruslan Deviatiiarov, Atlas of transcribed regulatory
elements in healthy and diseased human hearts, Online

6.

2022/5/19; Kenichi Miharada, Identification of tumour-derived
negative regulators of erythropoiesis towards novel therapies forcancer-related anemia, Online

7.

2022/5/26; Daisuke Kurotaki, Deciphering the cis-regulatory codeof inflammatory dendritic cell differentiation, Online

8.

2022/6/2; Takatsugu Ishimoto, Stromal amelioration boosts theefficacy of anti-PD-1 immunotherapy in TGF- ﾀ signaling-driven
fibrotic tumors, Online

9.

2022/6/9; Yosuke Tanaka, Identification of differentiation roots ofhematopoietic stem cells by multi-barcoding, Online

10. 2022/6/16; Benjy Tan Jek Yang, Dissecting the immunopathology
mechanisms of HTLV-1-associated diseases by single-cell RNAseq, Online

23. 2022/10/27; Atsuko Yonemura, Single-cell proteomic profiling of
malignant ascites in gastric cancer reveals the microenvironment
promoting peritoneal dissemination, Onsite and Online
24. 2022/11/10; Daisuke Kurotaki, Identification of master transcription
factors for pancreatic tumor-associated macrophages, Online
25. 2022/11/17; Johansson Alban, Functional HSCs preferentiallymigrate to spleen during hematopoietic regeneration., Onsite andOnline26. 2022/11/24; Nguyen Thi Kim Nguyen, Elucidating the role ofglycosylation in epidermal stem cell aging, Onsite and Online
27. 2022/12/1; Shintaro Funasaki, Hypoxia response pathway controls
tumor development in Xp11.2 translocation renal cell carcinoma.,
Online28. 2022/12/8; Hidenobu Mizuno, Intravital imaging of callosal
projection development in neonates., Online
29. 2022/12/15; Ma Wenjuan, Metabolic regulation in erythropoiesis bysystemic ketogenesis in fasted mouse., Online
30. 2022/12/22; Guojun Sheng, Amniote chorioallantoic membraneand mammalian placentation, Onsite and Online

11. 2022/6/23; Aiko Sada, Cellular and molecular mechanisms
regulating proliferative heterogeneity of epidermal stem cells,
Onsite and Online

31. 2023/1/12; Tomson Kosasih, The role of commensal bacteria on
HSC maturation in neonatal bone marrow, Onsite and Online

12. 2022/6/30; Yuichiro Arima, Therapeutic Potential of Ketone Body
Production, Onsite and Online

32. 2023/1/19; Kenichi Miharada, Maternal 27-hydroxylase activity isessential for fetal organ formation, Online

13. 2022/7/7; Amaya Jauregi Miguel, TBX3 acts as tissue-specificcomponent of the Wnt/ ﾀ-catenin transcriptional complex, Onsite
and Online

33. 2023/1/26; Masahiro Fukuda, Dynamics of gastric cancer cells invivo, Online

14. 2022/7/14; Tatsuya Morishima, Oncometabolite independent
phospholipid metabolic adaptation promotes IDH2 mutant AML
cell survival, Onsite and Online
15. 2022/7/21; Maolake Aerken, Glucocorticoid signaling delays
castration-induced regression in murine models of prostate cancer,
Onsite and Online
16. 2022/7/28; Xichen Hu, Downregulation of 15-PGDH enhanced
NASH-HCC development through the induction of T cell
exhaustion, Onsite and Online
17. 2022/9/8; Masaya Baba, Hypoxia response pathway controls
metabolic shift & tumor development in Xp11.2 translocation renal
cell carcinoma, Onsite and Online
18. 2022/9/15; Erna Raja, Fibulin 7 maintains epidermal stem cell
heterogeneity during skin aging and future directions, Online
19. 2022/9/22; Xu Yuqing, Regulating ketogenesis by blue light.,
Onsite and Online
20. 2022/9/29; Ahad Khalil Nezhad Zein Abad, Integrated Single-Cell
Multi-omics Reveals Commonalities between Human and Mouse
HSCs, Onsite and Online

34. 2023/2/2; Rao.S. Madhura, Ras-GAPs Control Dendritic
Development in Barrel Cortex Layer 4 Neurons, Onsite and Online
35. 2023/2/9; Terumasa Umemoto, Gultaminolysis regulates the fate ofhematopoietic stem cells under proliferation conditions, Online
36. 2023/2/16; Daisuke Kurotaki, Deciphering cis-regulatory codes ofinflammatory dendritic cell differentiation, Online
37. 2023/3/2; Mariko Morii, Functional and molecular consequences of
Trisomy 21 on hematopoietic stem cells, Onsite and Online
38. 2023/3/9; Masaya Baba, Drug discovery for lymphedema bytargeting FLCN/FNIP protein complex, Onsite and Online
39. 2023/3/16; Mizuho Ishikawa, Regulatory mechanisms of epidermal
stem cell heterogeneity by undulating structures, Onsite andOnline40. 2023/3/23; Akira Fujiyama, Effects of Ketone bodies on lifestyle
related diseases, Onsite and Online
41. 2023/3/30; Johansson Alban, Innate immune memory formationand maintenance in HSCs, Onsite and Online― 87 ―

42. 2023/4/6; Goro Sashida, Exposure to microbial products
followed by loss of Tet2 promotes myelodysplastic syndrome via
remodeling stem cells, Onsite and Online

61. 2023/10/5; Jie Bai, Understanding of Trisomy 8 stem cell function
and transformation by using a new Trisomy 8 model, Onsite andOnline43. 2023/4/13; Tatsuya Morishima, Mitochondrial translation regulates
terminal erythroid differentiation by maintaining iron homeostasis,
Onsite and Online

63. 2023/10/26; Alban Johansson, The mechanism for hematopoietic
stem cell migration from bone marrow to spleen isdynamicallychanged during hematopoietic regeneration, Onsite and Online

44. 2023/4/20; Yosuke Tanaka, A large scale paired-daughter cell
analysis reveals that hematopoietic stem cells exhibit different
division patterns according to their subtypes., Onsite and Online

64. 2023/11/2; Masaya Baba, Unraveling transcriptional regulation intranslocation Renal Cell Carcinoma, Onsite and Online

45. 2023/4/27; Takashi Senba, Cancer-associated fibroblasts
reuse cancer-derived lactate to maintain a fibrotic andimmunosuppressive microenvironment in pancreatic cancer,
Onsite and Online
46. 2023/5/11; People who went to Lund last month, Report on 20th
Anniversary Conference at Lund Stem Cell Center, Onsite andOnline47. 2023/5/18; Yuxin Wang, Akkermansia Muciniphila induces delayed
extramedullary hematopoiesis through cooperative IL-1R and TLR
signals, Onsite and Online
48. 2023/5/25; Spencer Yang Chong, Deciphering the regulatory
landscape of murine splenic response to anaemic stress at single
cell resolution., Online
49. 2023/6/1; Nguyen T. K. Nguyen, Elucidating the role ofglycosylation in epidermal stem cell aging, Onsite and Online

65. 2023/11/9; Mariko Morii, Premature aging and HSC defects in
Down syndrome., Onsite and Online
62. 2023/10/12; Rieko Asai, Coupling-uncoupling of cell flow andmidline morphogenesis in gastrulation, Onsite and Online
66. 2023/11/30; Aiko Sada, Stem cell division dynamics underlying
skin regeneration and aging, Onsite and Online
67. 2023/12/7; Daisuke Kurotaki, Differential roles of enhancer andpromoter in hematopoietic cell differentiation and gene expression,
online68. 2023/12/14; Mayumi Hirayama, Haploinsufficiency of Tie2 in blood
cells suppresses MDS progression, Onsite and Online
69. 2023/12/21; Hidenobu Mizuno, Spontaneous neuronal activity andthe formation of functional brain microcircuits, Onsite and Online

50. 2023/6/8; Ruslan Deviatiiarov, From cold torpor to rethinking
chicken embryo, Onsite and Online

70. 2024/1/18; Kiyoka Saito, Hematopoietic stem cell responses underacute anemic conditions, and the role of heterogeous nuclear
ribonucleoproteins (hnRNPs) in hematopoietic stem cells, Onsite
and Online

51. 2023/6/15; Maolake Aerken, Myokines induce pro-angiogenic
signaling in prostate cancer., Onsite and Online

71. 2024/1/25; Mayu Kawai, The divergent cell fates of neonatal bonemarrow hematopoietic stem cells, Onsite and Online

52. 2023/6/22; Shintaro Funasaki, Hypoxia response pathway controls
metabolic shift and tumor development in Xp11.2 translocation
renal cell carcinoma, Onsite and Online

72. 2024/2/1; Guojun Sheng, Chorioallantoic membrane: its formation,
maturation and use in cancer biology, Onsite and Online

53. 2023/6/29; Mayu Kawai, Dissecting the HSC heterogeneity in thedeveloping bone marrow, Onsite and Online

73. 2014/2/8; Kenichi Miharada, Disruption of maternal bile acidmetabolism leads to adverse pregnancy outcomes, Onsite andOnline54. 2023/7/6; Kenta Kikuchi, Analysis of inflammatory dendritic celldifferentiation and function, Onsite and Online
55. 2023/7/13; Hiroki Nagai, Avian pluripotency in vitro., Onsite andOnline56. 2023/7/21; Phung Hung Manh, IL1R2 maintains epidermal stem
cell heterogeneity to mitigate TPA-induced skin inflammation,
Onsite and Online
57. 2023/7/27; Lingfeng Fu, Pre-metastatic stromal activation in
uncontrollable hyperglycemic patients enhances hematogenous
metastasis., Onsite and Online
58. 2023/9/7; Yuqing Xu, Neonatal ketone surge consolidates
cardiomyocytes identity through histone modification, Onsite andOnline59. 2023/9/14; Ho pui Yu, Microbial Signal-induced Molecular
Reprogramming in HSCs and Machine Learning, Onsite andOnline74. 2024/2/15; Yosuke Tanaka, DNA-barcode lineage tracing identify
a super dormant subset in hematopoietic stem cells, Onsite andOnline75. 2024/2/22; Tomohiro Yabushita, Plcl1 regulates HSC quiescence
via intracellular Ca2+ alteration, Onsite and Online
76. 2024/2/29; Yuichiro Arima, Ketone body metabolism regulates
blood homeostasis., Onsite and Online
78. 2024/3/14; Tatsuya Morishima, The role of mitochondrial tRNA
modification in hematopoiesis, Onsite and Online
79. 2024/3/21; Kenta Kikuchi, Molecular mechanisms of inflammatory
dendritic cell differentiation, Onsite and Online
80. 2024/3/28; Goro Sashida, Hmga2 is vitalized to control
hematopoietic stem cell dynamics and drive regeneration in stressconditions, Onsite and Online

60. 2023/9/21; Wei Weng, ETV2 induces endothelial, but not
hematopoietic, lineage specification in birds, Onsite and Online― 88 ―

8. Appendix

8-6. List of Seminars & Symposia  • D5 Seminar1.

2017/4/19; Victor Appay, Institut national de la santé et de larecherche médicale (INSERM), The decline of the immune systemwith aging, Onsite

2.

2017/5/31; Goro Sashida, Kumamoto University, Epigenetic
dysregulation in leukemia, Onsite

3.

2017/6/21; Tomas HANKE, Oxford University, Attaching the Achilles
heel of HIV, Onsite

4.

2017/7/11; Yosuke Mukoyama, National Institutes of Health (NIH),
Building Heart and Circulatory System: New Questions andParadigms, Onsite

5.

21. 2019/3/20; Antoon Moorman, University of Amsterdam,
Development of the cardiac building plan, Onsite
22. 2019/4/10; Ben D. MacArthur, University of Southampton, Machinelearning of cell identities from single cell expression data., Onsite
23. 2019/5/15; Terumasa Umemoto, Kumamoto University, Energy
metabolism regulates hematopoietic stem cell divisions, Onsite
24. 2019/6/13; Long N. Nguyen, National University of Singapore, The
roles of lysolipid transporters for health and disease., Onsite
25. 2019/7/10; Yosuke Mukoyama, National Institutes of Health (NIH),
Macrophage control of brain development, Onsite

2017/9/13; Koichi Nishiyama, Kumamoto University, Understandingof angiogenic mechanisms as a multicellular morphogenesis,
Onsite26. 2019/8/28; Yuichiro Arima, Kumamoto University, Ketone Body
Metabolism in Health and Diseases, Onsite

6.

2017/10/25; Elisa Laurenti, University of Cambridge,
Heterogeneous properties of human hematopoietic stem cells,
Onsite27. 2019/9/11; Masanori Nakayama, Max-Planck-Institute for Heartand Lung Research, Digenic inheritance of mutations in EPH and
SLC26A4 in Pendred syndrome, Onsite

7.

2017/11/22; Pilhan Kim, KAIST, In Vivo Visualization of Dynamic
Cellular Phenotype by Real-Time Intravital Microscopy, Onsite

28. 2019/9/25; Masahiro Ono, Imperial College London, Investigatingtime-dependent dynamic mechanisms of T cell regulation by
single cell technologies, Onsite

8.

2017/12/14; Toshio Suda, Kumamoto University, Stem Cells andTheir Niche, Onsite

9.

2018/1/24; Hitoshi Takizwa, Kumamoto University, Stem cells under
inflammatory stress, Onsite

10. 2018/2/14; Guojun Sheng, Kumamoto University, Cellularmorphogenesis in development, Onsite
11. 2018/3/15; Masaya Baba, Kumamoto University, The molecularbasis of kidney cancer, Onsite
12. 2018/5/30; Motomi Osato, National University of Singapore, Runx
tales: a small transcription factor family and human diseases,
Onsite29. 2019/10/9; Pilhan Kim, KAIST, Real-time IntraVital Microscopy (IVM): In Vivo Live Cell Imaging Platform, Onsite
30. 2019/11/6; Ruby Yun-Ju Huang, National Taiwan University,
GRHL2: a pioneer factor, Onsite
31. 2019/12/18; Eui-Cheol Shin, KAIST, Medical Significance of IL-15Induced T Cell Activation, Onsite
32. 2020/1/15; Keisuke Horikawa, Australian National University,
Lymphoma mutations - The dark side of B cell development,
Onsite33. 2020/6/24; Masahiro Fukuda, National University of Singapore, invivo imaging 101, Online

13. 2018/6/15; Makoto Yawata, National University of Singapore,
Utilizing high-dimensional cytometry to investigate human NK cell
response mechanisms against multiple myeloma subpopulations,
Onsite34. 2020/7/8; Koichi Takahashi, The University of Texas MD Anderson
Cancer Center, Precision clonal mapping in acute myeloid
leukemia with single-cell genomics, Online

14. 2018/7/4; Victor Appay, Institut national de la santé et de larecherche médicale (INSERM), Exhaustion of primary immune
resources with old age, Onsite

35. 2020/8/18; Young Seok Ju, KAIST, Tracing Oncogene
Rearrangements in the Mutational History of Lung
Adenocarcinoma, Online

15. 2018/9/12; Ruby Huang, Department of obstetrics of Gynaecology,
NUH, The epigenetic landscape and 3D chromatin architecture ofepithelial-mesenchymal, Onsite

36. 2020/9/23; Masanori Nakayama, Max-Planck-Institute for Heartand Lung Research, The effort to fill the gap between academic
research and drug discovery, Online

16. 2018/10/31; Pilhan Kim, KAIST, Real-time Intravital Microscopy : In
Vivo Live Cell Imaging, Onsite

37. 2020/10/7; Kenichi Miharada, Lund University, Regulation ofproteostasis and lipid metabolism in hematopoiesis and fetal
development, Online

17. 2018/11/7; Toshio Suda, Kumamoto University, Purine metabolism
in the divisions of hematopoietic stem cells, Onsite
18. 2018/11/21; Gang Huang, Cincinnati Children’s Hospital
Medical Center, Insights into the Function of SETD2 and H3K36
Trimethylation in Leukemia, Chemo-resistance and Hematopoietic
Stem Cells, Onsite
19. 2019/1/8; Toshimi Yoshida, Massachusetts general hospital,
Harvard Medical School, Transcriptional regulation in early
hematopoiesis, Onsite
20. 2019/2/6; Hidenobu Mizuno, Kumamoto University, In vivo imaging
to understand the mechanisms of brain disorders, Onsite

38. 2020/11/4; Pilhan Kim, KAIST, Real-time IntraVital Microscopy:
Catch the Scene In Vivo!, Online
39. 2020/12/16; Pentao Liu, University of Hong Kong, Expanded
Potential Stem Cells: A New Tool for Basic and Translational
Research, Online
40. 2021/1/27; Knut Woltjen, Kyoto University, Precise gene editing inhuman iPSCs, Online
41. 2021/3/12; Jae Myoung Suh, KAIST, Hippo signaling regulates
adipocyte dedifferentiation and tissue homeostasis, Online― 89 ―

42. 2021/3/31; Nina Cabezas-Wallscheid, Max-Planck-Instituteof Immunobiology and Epigenetics, Regulation of dormant
hematopoietic stem cells, Online

60. 2022/10/14; Mako Kamiya, Tokyo Insitute of Technology,
Activatable Chemical Probes for Fluorescence Imaging of Cancer
and Raman Imaging of Plural Enzyme Activities, Online

43. 2021/4/21; Jinju Han, KAIST, microRNAs regulating neurogenesis,
Online61. 2022/11/30; Takaomi Sanda, National University of Singapore,
Molecular pathogenesis of T-cell acute lymphoblastic leukemia,
Online44. 2021/5/25; Injune Kim, KAIST, Regulation of vascular barrier
integrity in the retina and brain, Online
45. 2021/6/16; Masayo Takahashi, Vision Care Inc. & Kobe Eye Center
Hospital, Retinal Organoid Transplantation, Online
46. 2021/7/14; Keita Tamura, École Polytechnique Fédérale deLausanne (EPFL), Cortex-wide neural processing for goal-directedbehavior, Online
47. 2021/8/25; Masahiro Shin, University of Massachusetts Medical
School, Valves are a conserved feature of the zebrafish lymphatic
system, Online
48. 2021/9/29; Paola Betancur, University of California San Francisco,
An Introduction to the Biology of Cancer and the Therapeutic
Approaches Used for its Treatment, Online
49. 2021/10/13; Roland G. Huber, Agency for Science, Technology
and Research (A*STAR), Integrative modeling of viral genome
structures, Online
50. 2021/11/17; Zilong Wen, Hong Kong University of Science andTechnology, The Origin, Heterogeneity, and Maintenance ofMicroglia in Zebrafish, Online
51. 2022/1/26; Jay W. Shin, Agency for Science Technology andResearch (A*STAR), Genome Institute of Singapore (GIS), Building
the Human Cell Regulatory Atlas in Japan, Online
52. 2022/2/16; Xinyang Zhao, The University of Alabama at
Birmingham, Signaling via protein arginine methylation inmegakaryocyte differentiation, Online
53. 2022/2/22; Fabiana Perna, Indiana University, Mapping the cellsurface proteome of hematological malignancies to develop
innovative immune therapies, Online
54. 2022/3/23; Ly Vu, University of British Columbia, Toward targeting
post-transcriptional gene regulation in Leukemia, Online
55. 2022/5/18; Miki Ebisuya, EMBL Barcelona, SYNTHETICDEVELOPMENTAL BIOLOGY: Cross-species comparison andmanipulation of organoids, Online
56. 2 0 2 2 / 6 / 2 2; To m o his a To da, D e u t s c h e s Z e n t r um f ür
Neurodegenerative Erkrankungen (DZNE), Roles of long-lived
cellular constituents in the maintenance of brain, Online

62. 2022/12/21; Nami Sugiyama, ETH Zurich, Distinct contributionsof partial and full EMT to breast cancer malignancy, Onsite andOnline63. 2023/1/11; Masayuki Yazawa, Columbia University, Targeting Ion
Channels for Drug Discovery - Cardiac arrhythmia & COVID-19,
Onsite and Online
64. 2023/2/22; Kanae Ando, Tokyo Metropolitan University,
Mechanisms underlying Alzheimer’s disease and related
neurodegeneration: how tau proteins become toxic?, Onsite andOnline65. 2023/3/15; Nicolas Rivron, Harunobu Kagawa., AustralianAcademy of Science, Blastoids: modeling mouse and humanblastocyst development and implantation with stem cells, Onsite
and Online
66. 2023/5/17; Takahiro Masuda, kyushu University, Ontogeny andfunction of diverse brain macrophages, Onsite and Online
67. 2023/6/28; Mari Sato, Hokkaido University, Bone is hearing. Fat isseeing., Onsite and Online
68. 2023/7/19; Tiago C. Luis, Imperial College London, Role ofthe hematopoietic stem cell niche in re-establishing blood cellhomeostasis upon stress., Online
69. 2023/8/30; Jing Huang, National Institutes of Health (NIH),
Unveiling the Non-Transcriptional Roles of CBFB in Breast Cancer
Tumorigenesis and Treatment, Online
70. 2023/9/27; Akihiro Yoshimura, Keio University, Molecular
Mechanism of Cytokine Signal Regulation and Immune Tolerance
by SOCS and NR4a, Onsite and Online
71. 2023/10/17; Fanyan Wei, Tohoku University, Multifaceted RNA
modification in life and disease, Onsite and Online
72. 2023/11/9; Paul Liu, National Institutes of Health (NIH), RUNX1 in
CBFB-MYH11 leukemia and familial platelet disorder, Online
73. 2024/1/31; Takaaki Akaike, Tohoku University, Supersulfide biology
and translational medicine for disease control, Onsite and Online
74. 2024/3/27; Xin Sun, University of California San Diego, Building
and Rebuilding the Lung, Onsite and Online

57. 2022/7/27; Keiko Nomura, Tokyo Institute of Technology,
Mechanosensation mediated by PIEZO channel in the sensory
neurons and the vascular system, Online
58. 2022/8/24; Shannon Elisabeth, University of Chicago, Type 1 andtype 2 calreticulin mutations activate discrete unfolded protein
response pathways to drive myeloproliferative neoplasms, Online
59. 2022/9/12; Josephine Galipon, Keio University, Biogenesis
mechanisms: studying the morphology and molecular biology ofnon-model organisms in their natural habitat, Online― 90 ―

8. Appendix

8-7. List of Visiting Researchers

Hidekazu Watanabe, Visiting Assistant Prof.,
University of California, 2014/10/1 - 2015/3/31
Guojun Sheng, Visiting Prof., RIKEN, 2015/9/1 2015/9/30
Hitoshi Takizawa, Visiting Prof., University Hospital
Zurich and University, 2016/10/1 - 2016/12/31
Sarah Louise Rowland-Jones, Visiting Prof.,
University of Oxford, 2016/2/1 - 2018/1/31
Hideo Ema, Visiting Prof., Chinese Academy ofMedical Sciences, 2015/1/1 - 2018/3/31
Marcelo Kuroda, Visiting Prof., Tulane University,
2015/12/1 - 2019/3/31
Masayuki Iwasaki, Visiting Associate Prof., Stanford
University, 2016/1/1 - 2019/3/31
Brusic Vladimir, Visiting Prof., Grifith University/
Nazarbayev University, 2017/4/1 - 2019/3/31
Tsvee Lapidot, Visiting Prof., Weizmann Institute of
Science, 2017/4/1 - 2019/3/31
Michihiro Hashimoto, Visiting Assistant Prof.,
National University of Singapore, 2017/10/1 2019/3/31
Christian Schönbach, Visiting Prof., Nazarbayev
University, 2018/4/1 - 2019/3/31
Ayako Ishizu, Visiting Associate Prof., National
University of Singapore, 2016/4/1 - 2020/3/31
Mitsuhiro Endo, Visiting Associate Prof., National
University of Singapore, 2016/4/12 - 2020/3/31
Mahiti Macdonald Reuben, Visiting Lecturer, SaintFrancis University, 2018/1/16 - 2020/3/31
Nam Long Nguyen, Visiting Associate Prof.,
National University of Singapore, 2018/10/1 2020/3/31
Kenichi Miharada, Visiting Associate Prof., Lund
University, 2018/2/1 - 2021/3/31
Baeg Gyeong Hun, Visiting Associate Prof., National
University of Singapore, 2019/4/1 - 2021/3/31

Masanori Nakayama, Associate Prof., Max-PlanckInstitute for Hear and Lung Research, 2019/6/1 2023/3/31
Victor Appay, Visiting Prof., Institut national dela santé et de la recherche médicale (INSERM),
2020/2/1 - 2023/3/31
Hitomi Nagayama, Visiting Prof., National
Sanatorium Kikuchi Keifuen, 2021/4/1 - 2023/3/31
Mayu Kawai, Visiting Lecturer, National Institutes of
Health (NIH), 2021/10/1 - 2023/3/31
Ben D MacArthur, Visiting Prof., University ofSouthampton, 2014/10/1 - 2024/3/31
Momoko Yoshimoto, Visiting Associate Prof.,
Western Michigan University, 2014/10/1 - 2024/3/31
Yosuke Mukoyama, Visiting Prof., National Institutesof Health (NIH), 2014/10/1 - 2024/3/31
Gang Huang, Visiting Prof., University of Texas
Health Science Center at San Antonio, 2018/4/1 2024/3/31
Ruby Huang, Visiting Prof., National Taiwan
University, 2018/4/1 - 2024/3/31
Keisuke Horikawa, Visiting Associate Prof.,
Australian National University, 2018/4/1 - 2024/3/31
Bin Tean Teh, Visiting Prof., National Cancer Centre
Singapore/ Duke-NUS Medical School, 2019/4/1 2024/3/31
Shimakawa Yusuke, Visiting Prof., Institut Pasteur,2020/7/1 - 2024/3/31
Ito Keisuke, Visiting Prof., Albert Einstein College ofMedicine, 2020/8/1 - 2024/3/31
Takashi Semba, Visiting Lecturer, National
Sanatorium Kikuchi Keifuen, 2020/10/1 - 2024/3/31
Taichi Shimura, Visiting Associate Prof., Kozo
Keikaku Engineering Inc., 2020/12/1 - 2024/3/31
Shah Adil Ishtiyaq Ahmad, Visiting AssistantProf., Mawlana Bhashani Science and Technology
University, 2022/4/1 - 2024/3/31

Tan Shi Hao, Visiting Associate Prof., National
University of Singapore, 2019/10/1 - 2021/3/31

Tomohisa Toda, Visiting Associate Prof., FriedrichAlexander-Universität Erlangen-Nürnberg, 2022/4/1 2024/3/31

GouYoung Koh, Visiting Prof., Institute for Basic
Science, 2014/10/1 - 2022/3/31

Oleg Gusev, Visiting Associate Prof., Kazan Federal
University/ Juntendo University, 2022/5/1 - 2024/3/31

Masahiro Ono, Visiting Associate Prof., Imperial
College London, 2017/4/1 - 2023/3/31

Elisa Laurenti, Visiting Associate Prof., University ofCambridge, 2017/4/1 - 2025/3/31

Makoto Yawata, Associate Prof., National Universityof Singapore, 2017/5/1 - 2023/3/31

Pilhan Kim, Visiting Associate Prof., KAIST, 2017/4/1- 2025/3/31― 91 ―

Toshimi Yoshida, Visiting Associate Prof.,
Massachusetts General Hospital, 2017/4/1 2025/3/31
Masahiro Fukuda, Visiting Assistant Prof., DukeNUS Medical School, 2017/4/1 - 2025/3/31
Koichi Takahashi, Visiting Associate Prof.,
University of Texas, 2017/5/30 - 2025/3/31
Anthony D Ho, Visiting Prof., Heiderberg University,
2017/8/1 - 2025/3/31
Motomi Osato, Visiting Prof., Kumamoto Kenhoku
Hospital, 2019/4/1 - 2025/3/31
Nina Cabezas-Wallscheid, Visiting AssociateProf., Max Planck Institute of Immunobiology andEpigenetics, 2019/5/1 - 2025/3/31
Maiko Sezaki, Visiting Assistant Prof., Universityof Texas Health Science Center at San Antonio,
2022/6/1 - 2025/3/31
Yingke Wu, Visiting Associate Prof., Max Planck
Institute for Polymer Research, 2023/5/1 - 2025/3/31
Takatsugu Ishimoto, Visiting Prof., JapaneseFoundation for Cancer Research (JFCR), 2023/8/1 2025/3/31
Rieko Asai, Visiting Assistant Prof., University ofCalifornia, 2023/8/1 - 2023/9/30
Kanako Wakahashi, Visiting Assistant Prof.,
Fundación Centro Nacional de Investigaciones
Cardiovasculares (CNIC) Carlos V, 2023/10/20 2024/1/31
Yumiko Matsubara, Visiting Associate Prof., Keio
University, 2023/1/1 - 2025/3/31― 92 ―

8. Appendix

8-8. List of Advisory Board• Charles Bangham, BM, PhD (Imperial College London)• Gou Young Koh, MD, PhD (Korea Advanced Institute of Science and Technology (KAIST))• Richard Koup, MD (National Institute of Health (NIH))• Andrew McMichael, MD (University of Oxford)• Yosuke Mukoyama, PhD (National Institute of Health (NIH))• Daniel G Tenen, MD (National University of Singapore)― 93 ―

9.Editorial Note

We decided to recapitulate our history and publish it as IRCMS trajectory upon the 10-year anniversaryof the establishment of the IRCMS (International Research Center for Medical Sciences). We hadthought for a long time that we needed to do it, but once we actually started preparing, we realized
how difficult it is to revisit history. We would like to share with you some of the improvements andperspectives that we found during this process.
What troubled us in assembling our 10 years of accomplishments was the fact that past data was
not kept by the organization, perhaps because IRCMS is a special organization within Kumamoto
University and regular rules were exempted. However, records are also important to verify what
we have tried to do as a special (advanced) organization and to spread know-how that we haveaccumulated about how to run a special organization. It is desirable to keep records in a form thatmany people can refer to, rather than relying on individuals to keep progress and records.
What we noticed when we were putting this book together is that the member list was created in thesummer after the 2016 Kumamoto earthquakes. In disaster-prone Japan, we need to be preparedfor various risks in addition to normal organizational operations. Ensuring the health and safety of
our members is a prerequisite for the performance of our research. We also experienced COVID-19
pandemic from 2020. Our institute, where face-to-face research exchanges were very active, took adevastating hit. But that experience has prompted us to update our technology, bringing new normality
and new value. We collaborated with overseas institutions and researchers to organize online seminars,
providing many graduate students and young researchers with opportunities to learn and study.
The pandemic experience was also an opportunity to reflect on trust in science. What is true, whoshould we believe, and who are the professionals? We have been trying to find ways to communicate
science through our “Science and Me” and “Gateway to Life Science” seminar series. We hope that
this publication will provide more concrete information about our activities, achievements, and policies
to many people.
Through the creation of this Trajectory, we once again felt that the unique structure of IRCMS has been
established thanks to the tremendous efforts by the researchers and staff who have been involved
so far. Based on this history, we hope to present to you all the further achievements of our current
members in the next Trajectory.
Sincerely,
Hanami Sakai, Nanako Watanabe, and Yuichiro Arima― 94 ―