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INSTITUTE OF TROPICAL MEDICINENAGASAKI UNIVERSITY

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２００２

MISSION STATEMENT
Institute of Tropical Medicine, Nagasaki University

The tropics, the most ecologically diverse region on the Earth, presents an ongoing complexity of tropical diseases and other health problems. In view of the remarkable advances made in
the field of international exchange in recent years, it is imperative that these problems be addressed from a global perspective.
Based on this understanding, the Institute of Tropical Medicine, Nagasaki University, aims
to overcome tropical diseases, particularly infectious diseases, and the various health problems
associated with them, in cooperation with related institutions, to strive for excellence in the following areas:
１．Spear-head research in tropical medicine and international health
２．Global contribution through disease control and health promotion in the tropics byapplying the fruits of the research
３．Cultivation of the researchers and specialists in the above fields

General view of the Institute
Coverpage: Children after Sunday mass (Tanzania)

Preface
The Institute of Tropical Medicine, Nagasaki University, established in
1942, is a unique government-assisted institution for the research on tropical
medicine, both in the basic and applied fields. Its reorganization led to thefirst collaborative institute in medical science in Japan in 1989, and designation as one of the Centers of Excellence in 1995 by Ministry of Education, Science and Culture. Present organization of the institute is composed of 3 major
research fields (11 departments, 1 domestic visiting department, 1 overseas
visiting department), 2 centers, and 1 clinical unit.
According to the first external review in 1996, the institute worked out its Mission Statement as shown ona back cover. To fulfil our mission, we continue the research and other related activities. The highlights are
as follows.
＊Activities on the Mission; Spear-head research in tropical medicine and international health
１．Strategic researches have been conducted to develop the novel weapon against tropical diseases; analysis
of genomic structure of Japanese encephalitis and dengue viruses, structure and function of the receptors forbacterial enterotoxins, mechanisms for expression and activation of superoxide degenerating NADPH oxidase system, and immuno-genetical analysis of tropical diseases.
２．Epidemiological studies and control have been carried out on malaria, dengue fever / dengue hemorrhagic
fever, acute respiratory infections, Kaposi's sarcoma and schistosomiasis in Southeast Asia and Africa.
３．Environmental factors, such as vector and socio-economic problem, which cause the spread of tropical diseases in the developing countries have been studied.
Department of virology has been designated as WHO Collaborating Centre for Reference and Research on
Tropical Viral Diseases.
＊Activities on the Mission; Global contribution through diseases control and health promotion in the tropics
by applying the fruits of the research
１．Staffs have given their technical co-operation to disease control program in developing countries as WHO
short-term consultants, JICA experts and other consultants.
２．Some of our professors participate in Hashimoto Initiative, the global parasite control which is organized
and carried out under leadership of Japanese Government.
３．In 2001, the institute will carry out the intellectual and technical leadership as Developmental Project
Partner of JICA Malaria Control Project in Indonesia .
＊Activities on the Mission; Cultivation of the researchers and specialists in the above fields
１．Staffs of the institute conduct the doctorate degree course which belongs to Graduate School of Biomedical Sciences in Nagasaki University.
２．Since 1978, the institute offers a 3-month course of Tropical Medicine and Related Studies.
３．Since 1983, the institute holds one year training course for foreign participants entitled Research in
Tropical Medicine sponsored by JICA.
In addition, the institute has a Home page by which we educate the public why tropical medicine is important for the future well being of humankind and information on the tropical diseases in the world.
This pamphlet is one of our advocacy efforts to the public. It offers a brief but intelligible explanation on
our research activities and other related social activities. Any suggestions and supports will be gratefully appreciated.
May, 200２
Yoshiki Aoki M. D., D. M. Sc.
Dean and Professor
Institute of Tropical Medicine
Nagasaki University―１―

Contents
Preface…………………………………………………………………………………………１

Contents…………………………………………………………………………………………２

Historical Review………………………………………………………………………………３

Successive Deans of the Institute……………………………………………………………４

Organizational Chart…………………………………………………………………………５

Collaboration Research………………………………………………………………………６

Institute of Tropical Medicine Steering Committee………………………………………７

Institute of Tropical Medicine Collaboration Professional Committee
Scope of Activity………………………………………………………………………………９

Postgraduate School…………………………………………………………………………９Three-month Course on Tropical Medicine and Related Studies
Training Course in Research of Tropical Medicine
Public Lectures at the InstitutePublications…………………８…………………………９…………………………………………１
０………………………………………………………………１
０……………………………………………………………………………………１
０

Department of Virology………………………………………………………………………１
１

Department of Bacteriology…………………………………………………………………１
２

Department of Protozoology…………………………………………………………………１
３

Department of Parasitology…………………………………………………………………１
４

Department of Thermal Adaptation
Department of Biochemistry
Department of Pathology…………………………………………………………１
５…………………………………………………………………１
６………………………………………………………………………１
７

Department of Internal Medicine……………………………………………………………１
８

Department of Preventive Medicine and AIDS Research
Department of Vector Ecology & Environment
Department of Social Environment……………………………………………２０…………………………………………………………２
１

Department of Molecular Immunogenetics…………………………………………………２
２

Department of Internal Medicine（University Hospital）
Animal Research Center

Administration…………………………………２３………………………………………………………………………２
３

Research Center for Tropical Infectious Diseases
Central Laboratory…………………………………１
９…………………………………………２４……………………………………………………………………………２
５…………………………………………………………………………………２
５

Location map of the Institute of Tropical Medicine on Sakamoto Campusof Nagasaki University
Telephone Number…………………………………………………………………………２
６……………………………………………………………………………２７―２―

Historical Review
The Institute of Tropical Medicine, Nagasaki
University was originally founded in March 1942, as
the East Asian Research Institute of Endemics, inorder to perform basic and applied studies on endemic diseases in East Asia. At the beginning, mostof its research activities were field studies in mainland China, performed by several department such as
Pathology, Bacteriology, Internal Medicine and Dermatology of Nagasaki College of Medicine. Unfortunately, all the facilities and research materials werecompletely destroyed instantaneously along with theMedical School by the atomic bomb which explodedon August 9 th, 1945. As a result, development of
the institute and its research activities were severely inhibited.
In April 1946, the institute was named as the
Research Institute of Endemics attached to Nagasaki
College of Medicine, and moved to Isahaya City in
May in order to resume its research activities. In accordance with the Act on the Foundation of National
Schools in May 1949, the institute was renamed as
the Research Institute of Endemics, Nagasaki University. Becauce of the severe flood in Isahaya City,
the construction of the new building in Sakamotomachi, Nagasaki City was started in 1960, and the institute moved to new building in April, 1961. At thattime, there were only two departments, Pathology
and Clinics, however, since 1964, new departments
were established every Year, such as Epidemiology,
Parasitology, Virology and at the end of 1966, thefirst extension of the building was completed.
In June 1967, according to the partial alterationof the Act on the Foundation of National Schools, thename of the institute was changed to the present one,in order to perform basic as well as applied studies

on tropical medicine. At the same time, the Department of Internal Medicine of the institute with 20 bed
facilities was opened in the University Hospital. In
1974, Department of Bacteriology and ReferenceCenter as an attached facility were opened. In 1978,
the Department of Preventive Medicine suppoeted byvisiting staff and the Training Course of Tropical
Medicine were started. In 1979, Ward of Infectious
animals became Animal Research Center. In March
1980, the 2 nd extension of the main building was
concluded. In September 1983, the Training Course
in Research for Tropical Medicine by JICA was
opened. In 1984,Department of Protozoology was established. In July 1985, the 3 rd extension of thebuilding was completed. In 1987, Department ofMedical Entomology was established. In 1989, theinstitute was reorganized to a collaboration research
institute. In 1991, Department of Biochemistry wasadded. In March 1994, the 4 th extension of thebuilding was completed, and in April, 1994, the institute was reorganized to 3 research fields, Tropical
Microbiology, Pathogenesis and Clinical Sciences,and Environmental Medicine, with addition of 2 newresearch departments, Thermal Adaptation and Social Environment, and the institute has 12 research
departments at present. In 1995, the Institute wasdesignated as one of the Centers of Excellence inthe forefront of scientific research. In 1996, a newresearch department, Molecular Epidemiology, wasestablished under the Research Field of Microbiology to invite an overseas visiting professor. In 1997,
the Reference Center was abolished and in its place
the Information and Reference Center of Tropical
Medicine was established, then in ２００１，
it was abolished and in its place the Research Center for Tropical Infectious Diseases was established, symbolizing
continuous consolidation and re-organization of theInstitute.

―３―

Successive Deans of the Institute（East Asian Research Institute of Endemics）
Susumu Tsunoo

May．
４，
１
９
４
２-Aug．
２
２，
１
９
４
５

Kohei Koyano

Dec．
２
２，
１
９
４
５-Jan．
２
３，
１
９
４
８

Kiyoshi Takase

Jan．
２
４，
１
９
４
８-Aug．
３
１，
１
９
４
８

Noboru Tokura

Sept．
１，
１
９
４
８-May．
３
０，
１
９
４
９

（Research Institute of Endemics）
Noboru Tokura

May．
３
１，
１
９
４
９-Aug．
３
１，
１
９
５
８

Nanzaburo Omori

Sept．
１，
１
９
５
８-Nov．
３
０，
１
９
６
３

Hideo Fukumi

Dec．
１，
１
９
６
３-May．
３
１，
１
９
６
７

（Institute of Tropical Medicine）
Hideo Fukumi

June．
１，
１
９
６
７-Nov．
３
０，
１
９
６
９

Daisuke Katamine

Dec．
１，
１
９
６
９-Nov．
３
０，
１
９
７
３

Kaoru Hayashi

Dec．
１，
１
９
７
３-Nov．
３
０，
１
９
７
７

Tatsuro Naito

Dec．
１，
１
９
７
７-Nov．
３
０，
１
９
７
９

Daisuke Katamine

Dec．
１，
１
９
７
９-Apr．
１，
１
９
８
１

Keizo Matsumoto

Apr．
２，
１
９
８
１-Apr．
１，１
９
９１

Hideyo Itakura

Apr．
２，
１
９
９
１-Apr．
１，
１
９
９３

Mitsuo Kosaka

Apr．
２，
１
９
９
３-Apr．
１，
１
９
９
７

Akira Igarashi

Apr．
２，
１
９
９
７-May．
３
１，
２
０
０
１

Yoshiki Aoki

Apr．
１，
２
０
０
１-Up to the present―４―Organizational Chart
Department of Virology
Department of Bacteriology
Research Field ofMicrobiology

Department of Protozoology
Department of Parasitology
Department of Molecular Epidemiology(Overseas Visiting Professor)
Department of Thermal Adaptation
Department of Biochemistry

Research Field ofPathogenesis
and Clinical
Sciences

Department of Pathology
Department of Internal Medicine
Department of Preventive Medicine
and AIDS Research
Department of Vector Ecology& Environment
Department of Social Environment

Dean

Faculty
Meeting

Research Field ofEnvironmental
Medicine

Department of Environmental Physiology
Attached
Facilities

Animal Research Center
Research Center for
Tropical Infectious Diseases
Central Laboratory
General Affairs Unit

Administration

Accounting Unit
Collaboration Research Unit

University
Hospital

Clinical
Department

Department of Internal Medicine―５―These activities of the institute are expected toCollaboration researchcontribute to remarkable progress of tropical medicine.

The institute has conducted research in the field

The senior staff composed of professors of theof tropical medicine for the past ６
０ years since its

institute and the executive committee make plans forestablishment in１
９４
２．The institute was reorganizedresearch work. The steering committee and the col-to make extensive collaboration with other universi-

laboration professional committee composed of expe-

ties and institute in the field of interdisciplinary

rienced scientists coordinate the various collabora-

tropical medicine with molecular biology, entomology,tion researchs.

anthropology, social medicine, etc.

Collaboration Research Sytem

Steering Committee

The senior
staff（Professors）

Collaboration professional
committee

Executive committee―６―

Institute of Tropical Medicine Steering Committee
The University of Tokyo

Professor Emeritus

National Institute of Infectious
DiseasesHonorary Post

Nagasaki University

Professor Emeritus

Manabu Sassa
Akira Oya
Keizo Matsumoto

The Institute of Medical Science
The University of Tokyo

Director

Ken-ichi Arai

Research Institute for Microbial
Diseases Osaka University

Professor

Yoshitake Nishimune

The International Medical Center

President

Yoshio Yazaki

Japan International Cooporationof Welfare Services

Senior Medical Adviser

Takashi Wagatsuma

Kobe University

Professor

Shigeaki Sato

Japanese Foundation for AIDS Prevention

Director

Tadao Shimao

Professor

Keiji Ide

Nagasaki University
Faculty of Economics
Graduate School of Biomedical Sciences

Dean

Institute of Tropical Medicine

Dean

〃
〃
〃
〃
〃
〃
〃
〃
〃
〃
〃
〃

Koutaro Taniyama◎Yoshiki

Aoki

Professor

Kouich Morita

Professor

Toshiya Hirayama

Professor

Hiroji Kanbara

Professor

Michio Nakamura

Professor

Takuya Iwasaki

Professor

Tsuyoshi Nagatake

Professor

Naoki Yamamoto

Professor

Masahiro Takagi

Professor

Tsutomu Mizota

Professor

Kenji Hirayama

Professor

Masaaki Shimada

Professor

Kazuhiko Moji◎：Chairman―７―Institute of Tropical Medicine Collaboration Professoional Committee
Gunma University

Professor

Mamoru Suzuki

Kyusyu University

Professor Emeritus

Isao Tada

Kobe University

Professor

Haku Hotta

Kumamoto University

Professor

Hiroshi Maeda

University of the Ryukyus

Professor

Shigeo Nonaka

Oita Medical College

Professor

Kumato Mifune

University of Occupational andEnvironmental HealthProfessor

Takekiyo Yoshimura

Osaka International University

Professor

Yuji Yamamoto

Keio University

Professor

Yoshiyasu TakefujiNagasaki International University

Professor

Taiichirou Takemoto

Professor

Shigeru Katamine〃

Professor

Nobuyuki Kobayashi

Institute of Tropical Medicine

Professor◎Tsuyoshi Nagatake〃

Professor

Kouichi Morita〃

Professor

Toshiya Hirayama〃

Professor

Hiroji Kanbara〃

Professor

Yoshiki Aoki〃

Professor

Michio Nakamura〃

Professor

Takuya Iwasaki〃

Professor

Naoki Yamamoto〃

Professor

Masahiro Takagi〃

Professor

Tsutomu Mizota〃

Professor

Kenji Hirayama〃

Professor

Masaaki Shimada〃

Professor

Kazuhiko Moji

Nagasaki University
Graduate School of Biomedical Sciences◎：Chairman―８―

Scope of Activities

Postgraduate School

Based on the following research organization

In April，
２００２the system of Doctor Courses in Na-

and intimate linkages with other research institutes

gasaki University has been changed. In the new sys-

and universities, the institute aims at accomplishing

tem the Graduate school of Medical Science, Dental

a mission

Science and Pharmacology were integrated into theSpear-head research in tropical medicine

and international health .

Graduate School of Biomedical Science consisting of● To study comprehensively the tropical diseases

four courses. All the departments in the Institute be-which are raging in the developing countries, re-long to the Course of Infection Research. Students

search organization of the institute consists of 3

who want to study tropical medicine in the Institutemajor research fields which deal with the classicalare required to apply to the office of the Graduate

triad of

School of Biomedical Science through the professor

human-agent-environment determinantsof infectious diseases and Research Center forof each department.

Tropical Infectious Diseases.
● The research of tropical medicine faces the inevi-

Three-month Course on Tropical
Medicine and Related Studies

table fact that the bench is in the bush . Therefore the institute has a close linkage with the overseas institutes in Asia, Africa and South Americas
and continues the joint studies. Memorandums of

The course aims to provide participating per-academic exchange programs were signed between

sons with a better understanding of the health prob-

Nagasaki University and 3 overseas institutes. Inlems in tropics, to increase their ability to cope with

2000, the exchange program under the core univer-them, and to give a fuller knowledge of the culturessity system by JSPS was newly established be-

and the life of the people in tropics.
Physicians and professionally qualified persons

tween our Institute and National Institute of Hy-

working in health and related fields may apply for ad-giene and Epidemiology in Vietnam.
● The prevalence of tropical diseases depends on

mission to the course. Fifteen candidates are ac-

the geographic, social end economic factors.

cepted to the course in a year. The course consists ofTherefore the institute has established the special

１３weeks （June-August） of instruction and exami-

research system which helps forward the multidis-

nation. Teaching is undertaken by the full-time staff

ciplinary studies on tropical diseases.

and guest lecturers.
The course provides lectures and laboratory andfield practices in virology, bacteriology, protozoology, parasitology, medical entomology, environmental
physiology, biochemistry, pathology, genetics, epidemiology, human ecology, social medicine, internal
medicine, and geography and culture in tropics. Candidates who completed successfully the course areawarded the Diploma in Tropical Medicine.

Adomission ceremony in２
０
０
２

―９―

Training Course in Researchof Tropical Medicine

Public Lecturesat the InstituteArrangements for conducting the course in this In-

Annually, the Institute of Tropical Medicine holds

stitute are administered by Japan International Coopera-

public lectures for the citizens. The lectures address is-tion Agency, commissioned by the Government of Japansues pertaining to travellers to regions endemic to tropi-

to execute Technical Cooperation Programs from １９８３．

cal diseases. It is intended to prepare the public to main-

This course is conducted by the Government of Ja-

tain good health abroad. The other aim is to open our in-

pan as a part of its Technical Cooperation Programs fortellect and knowledge to the public.

developing countries with a view of contributing to upgrading their standards in tropical medicine and to pro-

Publications

moting friendly relations to the countries.
The purpose of this course is to help the control ofendemic and epidemic diseases infesting the Torrid Zone.

Tropical Medicine

Through the guidance of the staff of the Institute, the par-This is a quarterly journal with publications of origi-

ticipants will enrich their fundamental knowledge andnal articles from the Institute. The journal was first pub-practical techniques for various medical problems in thelished in March １
９５９ as Endemic Diseases Bulletin oftropics.

Nagasaki University . In１９６７，the name was changed

Individual participants will study on a selected subject in the responsible department for a year. Capacity ofto Tropical Medicine . This year, publication was suspended.

each department is normally limited to one person.
Institute of Tropical Medicine, Nagasaki University
The first English guide for foreign visitors wasprinted in １９７１. A revised edition is published every
year.

Adomission ceremony in２
０
０
１

―１
０―

Department of Virology

Activities as a WHO Collaborating Center
By the letter from Dr. S. T. Han, former Direc-

This Department has been conducting basic andtor of WHO Regional Office for the Western Pacificapplied research on mosquito-borne flaviviral dis-

(WPRO) dated 23 November 1993, the Department ofeases such as Japanese encephalitis (JE), dengue fe-

Virology, Institute of Tropical Medicine, Nagasakiver (DF) / dengue hemorrhagic fever (DHF), as well

University was designated as WHO Collaborating

as hepatitis C.

Center for Reference and Research on Tropical Viral Diseases. On 9 August 1994, Inauguration Cere-

Analysis on the structure and function of JE and den-mony was held at Pompe Hall by the presence of Dr.

gue viral genome

Han and approximately 120 guests, which was fol-

Nucleotide sequence analysis and molecular expression of JE and dengue viral genome have been
carried out together with long PCR techniques in order to study molecular epidemiology, epitope analysis, biological activity of nonstructural proteins, andvirulent viral gene responsible for viral pathogenesis.

lowed by the WHO Workshop

Epidemiology andControl on DF /DHF and JE . The Department hasreceived WHO fellows from Vietnam, Fiji, and thePhilippines, and dispated WHO short-term consultants on the subjects relevant to its terms of reference. Dr. Kouichi Morita was appointed as the Regional Advisor on Communicable Diseases, WHOWPRO, from 16 May 1995 to 15 May 1998. Accord-

Analysis on apoptosis induced by flavivirus infections.
Flavivirus infection induces apoptosis of the infected cells. Characterization of apoptosis and its

ing to the letter from Dr. Shigeru Omi, present Director of WHO-WPRO, the Collaborating Center
was extended to 23 September 2003.

relation to pathogenecity has been studied in cell line

Professor

Kouichi Morita

and animal model.

Research Associate

Futoshi Hasebe

Development of rapid diagnosis of flaviviral diseases

Research Associate

Shingo Inoue

Guest Research Fellow

Maria del Carmen Parquet

Viral genome detection and identification by

Senior Research Assistant

Tomomi Yamaguchi

PCR, preparation of diagnostic antigen from infected

Technician

Kazumi Jodai

cell culture fluid, and simplification of IgM antibody

Postgraduate student

Afjal Hossain Khan

detection have been carried out.

Postgraduate student

Edward G. Mathenge

Newly identified JE neutralizing epitope 503

Postgraduate student

Thai Hong Thicam

Postgraduate student

Yu Fuxum

Ronpaku Fellow

Paresh Sumatilal ShahJICA Student

Gissel Garcia Menendez

P 2 Level laboratory―１１―Department of Bacteriology
Our major research interest is to elucidate the
etiologic agents isolated from pathogenic bacteria
related to the worldwide emerging and re-emerging
diseases.
Studies on the cellular and molecular mechanisms of diarrhea induced by bacterial enterotoxins and Salmonella :Aeromonas sobria hemolysin is important in thepathogenesis of diarrhea caused by this enteropathogenic
bacterium .
Glycosylphosphatidylinositolanchored glycoprotein was identified as a receptor
for A. sobria hemolysin on Intestine 407 cells. (Ref.
Microb. Pathog. (1999) 27 : 215)
Focusing on the molecular mechanisms of the diarrhea induced by heat-stable enterotoxins (STa) ofenteropathogenic bacteria, we are studying 1) interaction of Escherichia coli heat-stable enterotoxin
with its receptor and 2) activation of guanylate cyclase (GC-C) by STa. (Ref. Eur. J. Biochem. (1999)
263: 338) Inflammation caused by Salmonella is also
investigated (J. Biol. Chem. (2001) 276.３
０５２
１)Studies on the pathogenesis of Helicobacter pylori :To investigate a potential mechanism of how H.
pylori establishes infection, we investigates the hostparasite relationships of H. pylori , focusing on vacuolating cytotoxin A (VacA) and Cag pathogenicity island (CagPAI).
1) VacA exposed to alkaline or acid conditions,with subsequent neutralization, exhibits enhanced
vacuolating activity; the acid or alkali-activated
VacA appears to bind a cell surface receptor proteinof 〜250 kDa. N-terminal and internal amino acidsequence is consistent with the hypothesis that p 250
is RPTPβ. Phorbolmyristate (PMA, TPA) induces
differentiation of the human leukemic cell line HL-60
into cells with macrophage-like characteristics andenhances the susceptibility of HL-60 cells to VacA.
PMA induced expression of RPTPβ mRNA and protein as determined by RT-PCR and indirect immunofluorescence studies. Vitamin D 3 and IFN-γ,
which stimulate defferentiation of HL-60 cells into a
monocyte-like cells, also induced VacA sensitivity
and expression of RPTPβ mRNA, whereas 1.2%
DMSO and retinoic acid, which stimulated the maturation of HL-60 into granulocyte-like cells did not.
RPTPβ anti-sense oligonucleotide inhibited induc-tion of VacA sensitivity and expression of RPTPβ.
Double immunostaining studies also indicated that
newly expressed RPTPβ colocalized with VacA in
PMA-treated HL-60 cells. BKN-21 cells transfected with the RPTPβ cDNA acquired VacA sensitivity. All data are consistent with the conclusionthat acquisition of VacA sensitivity by PMA-treated
HL-60 cells results from induction of RPTPβ, a protein that function as the VacA receptor. (Ref. J. Biol.
Chem. (1999) 274: 36693, J. Biol. Chem. (2000) 275:
15200) The detail mechanism of VacA action was
briefly clarified (Ref. J. Clin. Inv. (2001) 107: 15200)
2) Human β-defensin-2 (hBD-2) is an antimicrobial peptide which belongs to one of the most important host defence system against bacterial infection
in several epithelial tissues. We studied the effect of
H. pyloli on the expression of hBD-2 mRNA in MKE
45 gastric mucosal cells. H. pylori , but not culture
filtrate, increased hBD-2 mRNA level in MKN 45
cells, whereas thus inductive effect of H. pylori was
not detected when Intestine 407 cells were incubatedwith H. pylori . Among the tested strains of H. pylori ,which lacks Cag PAI, did not induce hBD-2 mRNA in
MKN 45 cells. These results suggested that cag PAIof H. pylori is important for inductive expression of
hBD-2 mRNA through NF-KB activation in MKN 45
cells.
Exposure of MKN 45 cells to Salmonella typhimurium, S. enteritidis , S. typhi , and S. dublin ,but not Escherichia coli ML 35, resulted in remarkable induction of hBD-2 mRNA. (Ref. Biochem.
Biophys. Res. Commun. (1999) 283: 770, Infect.
Immun. (2000) 68: 1806, Cell. Microb. (2001) 3:
115)
Studies on the development of cholera vaccine:The overexpression of fimbriae of Vibrio cholerae
O 1 is under study for use in cholera vaccine trial.
(Ref. Microbiol. Immunol. (2000) 44: 439)

Cytotoxicity of VacA toxin through its binding toreceptor-protein tyrosine phosphataseβ―１２―Professor
Toshiya Hirayama
Assistant professor
Yoshio Ichinose
Research Associate
Masahiko Ehara
Research Associate
Akihiro Wada
Research Associate
Akitoyo Ichinose
Technologist
Mamoru Iwami
Technician
Kayo Honda
Postgraduate Student Takahiro Kimura
Postgraduate Student Masaaki Nakayama

Laboratory

Study of trypanosomes

Department of Protozoology

１）Functions and expression mechanism of transsialidase.

Our main purpose is to clarify infection mecha-

２）Adaptation mechanisms of Trypanosoma species
to environments.

nisms of intracellular protozoan parasites.

３）Modification of infected host-cells by Trypano-soma cruzi .

Study of malaria parasites

４）Simple diagnostic methods for Chagas' disease in１）Specific immune reaction in malaria.

endemic fields.

２）Surviving strategies of Plamodium falciparum inmammalian hosts.
３）Epidemiology of human malaria.

Other studies１）Molecular epidemiology of pathogenic strains ofEntamoeba histolytica .
２）Epidemiology of cryptosporidiosis

Laboratory for culture

Plasmodia sporozoites from ruptured oocysts in
Anopheline mosquito

Professor

Hiroji Kanbara

Assistant Professor

Haruki Uemura

Research Associate

Shusuke Nakazawa

Technician

Miki Kinoshita

Technician

Kurenai Tomimaru

Technician

Kazumi Yoshinaga

Postgraduate Student

Mie Kato

Postgraduate Student

Katsunori Shinohara

Postgraduate Student

Ton That Ai Long

Postgraduate Student

Chaturong Putaporntip

Postgraduate Student

Mohammed Nasir Shuaibu

Postgraduate Student

Maria Cecilia Huaman

Postgraduate Student

Toshio Miyazaki

Postgraduate Student

Sandra Ines Juarez

JICA Student

Acleus Malinzi Rutta

JICA Student

Fidel Angel Nunez Fernandez

Amastigotes（left）and trypomastigotes（right）of
Trypanosoma cruzi―１３―

Department of Parasitology
The research activities are concentrated on filariasis, schistosomiasis, and intestinal helminthiasis
which constitute the major public health problems in
the tropics.
Filariasis

Brugia malayi (Cheju strain, periodic form),B.
pahangi and the vector mosquito,Aedes aegypti (Liverpool strain)have been maintained in the laboratory
for many years. Highlights of recent studies are as
follows.
１）Development of a simple and sensitive method for
determination of serum concentration of ivermectin(IVM) and diethylcarbamazine (DEC):IVM and DEC modified partially in their chemical structure successfully produced the antibody
against drugs. Therefore the serum concentration(5ng/ml)of IVM and DEC can be determined by EIA.
２）Screening of antifilarial drugs from medical
plants:Vernonia amygdalina from Africa, Neurolaena
lobata from Guatemala and Cardiospermun halicacabum from Thailand, were effective in vitro on B.
pahangi adult worms and microfilariae.
３）Epidemiology and control of bancroftian filariasis:
A research project was carried in Kwale, Kenya,in cooperation with Kenya Medical Research Institute (KEMRI) during the period of 1990 and 1996.
Transmission potential and morbidity were studied.
Mass-chemotherapy with combination of DEC andNaHCO３ was evalualed.

follows.
１）Swimming behavior of miracidia:
cAMP is involved in the control of ciliary beating and chemotaxis of miracidia.
２）Mechanisms of penetration of cercariae into skin:
The studies suggest the involvement of protein
kinase C in proteolytic enzyme release from cercariae.
３）Epidemiology and control of S. haematobium infection:
Since 1981, the research project on Schistosomiasis haematobia was carried out in Kwale, Kenya,in cooperation with KEMRI for 20 years. The highlights of our studies are human water contact study,
cercarial concentration in natural water, ecology of
Bulinus globosus , usefulness of urinary reagent
strips, new immunodiagnostic test (urine ELISA,
modified COPT) , effect of piped water supply, KAP
study (knowledge, attitude and practices), health education, morbidity studies using ultrasound, environmental modification for snail control, prevalence of
bladder cancer and liver fibrosis, and qualitative andquantitative studies on difficulty of urination in thecommunity.
Intestinal helminthiasis
Since 2000, epidemiologial studies on intestinal
helminthiasis has been taken up. The studies aim toshow that mode of transmission is different in countries and main determinant is human behaviour.
Professor
Assistant Professor
Research Associate
Technologist
Technician
Postgraduate Student
Postgraduate Student
Postgraduate Student
Postgraduate Student
Postgraduate Student

Schistosimoasis

Schistosoma mansoni (Puerto Rican strain and
Kenyan strain), S. haematobium (Kenyan strain)andsome strain of vector snails have been maintained in
the laboratory. Highlights of recent studies are as

Examination of difficulty of urination by using Uroflowmeter in an endemic area of Kenya

Yoshiki Aoki
Yasunori Fujimaki
Kanji Watanabe
Mitsumasa Miura
Satomi Tominaga
Tomoharu Ohki
Hidehiko Yamauchi
Hiroshi Matsuyama
Gunawardena Nipul Kithsiri
Teruyo Kusaba

Examination of contamination of soil by eggs and larvaeof intestinal helminthis in Vietnam―１４―

Department ofThermal Adaptation
The department was established in１
９９
４to study on
the influence and damage of tropical environment on
human and animals. Then study result is applied inprotection and care of tropical disease for improving
infection and symptom.
Study on short and long term heat acclimatization of humanOn the short term heat acclimatization, it experiment to expose Japanese to heat in environmental
chamber for period from spring to summer. They
sweat too mach and high electrolyte concentration in
the sweat for surplus reaction, so they have big damage as compensation of body temperature control.
Heat loss responses of Tropical inhabitants arestudied in the short term heat acclimatization. Their
nonevaporative heat loss by blood circulation are effective, and their sweat is a little with low electrolyte concentration as comparison with Japanese it.
Therefore, they sweat minimum volume, and haveheat tolerance(Left figure).
Victims by heat stroke decrease, who are mainly
children and old men. Society and economy develop
by ability adaptation for heat acquired, which increases working ability without discomfort and fatigue.
A study of effective defense method from ultraviolet
rays
Ultraviolet rays (UV) contained by Sun lightcause various damage in the living body, and UV is alarge quantity and strong power in the Tropical Zone
and the mountains in particularly. Therefore,thecreatures on earth acquired various defense methods
for ultraviolet rays harmful to body in a process ofevolution.
We have been promoting the study of defense
methods from ultraviolet rays in wild animals which
inhabit in a low latitude area, the desert and mountains (Right figure: black layer of body hair absorbs
UV) in particular.
For the purpose, we study effective defense
mechanisms for ultraviolet rays in wild animals, thenwe design the best defense method against ultraviolet
rays with nature and harmless.
The influence of change in biophylaxis functions induced by ultraviolet radiation to infection of tropical disease
Skin cancer occurs by strong ultraviolet radiation for a long term. The exposure to more little
mount of ultraviolet rays may be influence biophylaxis function to infection. UV in the Tropical Zone
are strong, and many persons suffer from infectious
disease. There are approximately 250 million patients with schistosomiasis in Tropical Zone.
In our experiment on infection of Schistosoma
mansoni cercariae to mouse, ultraviolet radiationgroup by ultraviolet lamp (UV-B) was statistical difference in the number of Schistosoma mansoni cercariae invaded into skin and recovery the adult
warms compared with nonirradiation group. We experiment the influence of ultraviolet rays in Sun
light on it now.
This study elucidates influence of ultraviolet
rays for infectious disease and contributes to the infectious disease control, in particular,in the Tropical
Zone with strong ultraviolet rays.
Associate professor (Additional post)
Nobu Ohwatari

Experiment of thermal sweating in the environmental
chamber―１
５―

Pika inhabiting the mountains of 3200!

For the expression of gp９１phox in these cells, weDepartement of Biochemistrypreviously suggested a common transcriptional activator which requires the position-５３ based on an

Our research interest is focused on the molecu-

analysis of a novel patient with chronic granuloma-

lar events ocurring in inflammatory cells for the de-tous disease. HAF-１ and PU．
１were indentified as

fense against invading microbes.

Reactive oxygencandidates for this activator. We, therefore, exam-

species are essential for killing most of bacteria,

ined ６０ fragments with onebase substitutions neigh-fungi, and parasites. We are therefore investigating

boring to-５３，and found two sequences;one has a mu-mechanisms for the expression and activation oftation at-５６and impairs the binding of HAF-１， andsuperoxide-degenerating NADPH oxidase system.

the other mutation at-５０ and impairs the binding of

GATA-３ as the Eosinophil-specific Repressor for the

PU．
１The-５０mutant promoter but not-５６mutant one

Expression of gp９１phox, an Electrontransferring Compo-

exhibited decreased reporter activity, indicating

nent in Phagocyte NADPH Oxidase System

PU．
１，but not HAF-１，to be an essential activator

In a systematic search for cis-elements regulat-

for the expression of the gene in those cells （Fig．
１）．

ing gp９
１phox exression in eosinophil lineage, we identi-

A discovery of a similar patient with a point mutation

fied an inhibitory element containing a GATA con-at-５２ which abolished the binding of PU． １ con-sensus site at the proximal promoter and GATA-３

firmed our conclusion.

as the specific protein binding to that site. Two-base

Our future aim is to apply these findings to in-pair substitution at the consensus site abolished in-innovation of techniques to control tropical diseases

hibition of the promoter activity in eosinophil-and allergy.

committed HL６０-C１５ cells, indicating that the
GATA-３ binding to the site is a repressor in theProfessor

Michio Nakamura

cells. Because eosinophil is the only cell expressing

Assistant Professor

Atsushi Kumatori

GATA-３ among human phagocytes and B lympho-

Research Associate

Shoichi Suzuki

cytes, GATA-３ is an eosinophil lineage-specific re-Technologist

Toshiyuki Moriuchi

pressor of gp９
１phox gene.

Postgraduate Student

Yoshito Fujii

Postgraduate Student

Maki Yahiro

Postgraduate Student

K. A. Deepa

JICA Student

Jorge Frage Nodarse

PU．
１but not HAF-１ is a Common Activator for
the Expression of gp９
１phox in Neutrophils, Monocytes and
B Lymphocytes-56G m-50G m
-53T m
Wild
pXP2
0

100

200

300

400

500

Relative LUC Activity

Fig．
１．Reporter gene expression driven by various mutant gp９
１phox promoters―１６―Department of Pathology /Division of Clinical Investigation

Virus infections of the central nervous system.
In the southeast and east Asia flavivirus encephalitis, such as Japanese encephalitis and tick-

In１
９
７０，
the Late Professor Toyosuke WATAN-

borne encephalitis, and rabies are still big problems.

ABE classified tropical diseases into４groups:

Recently, enterovirus ７１infection among children is

１．Diseases caused by pathogens, uniquely present

frequently associated with serious neurologicalmanifestaions in these areas. We are investigatingin the tropical areas.
２．Diseases induced by environmental factors suchthe clinicopathological aspect of these infections andalso performing the animal experiments to establish

as tropic climate.
３．Diseases specific for some habitants in thetropical areas due to their high susceptibility.
４．Diseases difficult to prevent in developing coun-

their pathogenesis and prevention.
Carcinogenesis in papillomavirus infection.
Neoplasms, dysplasia and condylomas of the mu-tries.
Now, we are investigating the pathogenesis ofcosa and skin are investigating for the presence oftropical diseases based on this concept, especiallypapillomaviruses using the molecular and pathologi-

for infectious diseases caused by viruses, and aim tocal methods. In addition, we are developing an animal

establish the fundamental basis of their prevention

model of mucosal carcinogenesis using hamster.

and treatment.

Virus infections of the respiratory tract.

Activities

Human cases with airborne-infections such as
influenza and measles are studied for pathological di-

Pathological diagnosis of infectious diseases.
We are developing various antibodies and molecular probes available for diagnosis of infectious
diseases on the clinical specimens obtained at cytol-

agnosis and their pathogeneses.
Pathology of viral hepatitis.

ogy, biopsy, necropsy and autopsy. We are also per-Epidemiological and histopathological studies

forming various animal experiments to obtain posi-

are being done in the southeast Asia for viral hepati-tive controls for this analysis and to analyze theire

tis and its sequelae.

pathogeneses.
Professor

IWASAKI, Takuya

Associate Professor TORIYAMA, Kan
Research Associate SENBA, Masachika
Research Associate HAYASAKA, Daisuke

Legend: The central nervous tissue of cynomolgus monkeys inoculated with enterovirus７１(EV７１）． Left: Hematoxylin−eosin stain.
Right: Immunostaining for EV７
１capsid antigens.

―１
７―

Technician

FUKUDA, Akemi

JICA Student

Driss Ahmed MOULAYE

Department of Internal Medicine%．Analysis of the risk factor for nosocomial pneumonia in mouse experimental model

Our main studies are described in the followings.
!．Comparative study on respiratory infections between other countries and Japan
Our department has promoted collaboration
study on respiratory infections with Thailand, Bangladesh, Vietnam, Uganda and Kwait. Since then thepathogens of respiratory infections has been recognized. As a result the antimicrobial treatment has
been appropriately modified.

&．Collaboration study in Uganda on the treatment of
AIDS patients with infections(tuberculosis, cryptococcal
meningitis and e. t. c)
Since 1990 we have investigated the condition of
AIDS in Uganda to save patients complicated with
infections. Caollaboration project has been with the
School of Medicine, Makerere University, Uganda.
'．Basic and clinical studies on influenza"．The laboratory and clinical studies on bacterial respiratory infections(．Chinical study on acute respiratory infections andbacterial meningitis among the children in Bangladesh#．Studies on anti-inflammatory mediator therapy inchronic respiratory infections
Our studies have revealed that the neutrophil
activator, IL-8 and neutrophil elastase, play a very
important role in chronic respiratory infections. The
studies for anti-mediator therapies are undergoing.

)．Study on Bacterial adherence of respiratory infection.

$．Basic and clinical studies on nosocomial infections
by Staphylococcus aureus
Staphylococcal infection is one of the serious
problems of nosocomial infections. We established amethod for the management of nosocomial infections.
Although we could reduce the occurrence of
MRSA infections, there are various problems concerning this organisms particularly its pathogenecity.

Laboratory of bacterial research

Professor
Tsuyoshi Nagatake
Associate Professor
Kazunori Oishi
Research Associate
Hideaki Amano
Research Associate
Kiwao Watanabe
Postgraduate Student Mariko Saito
Postgraduate Student Jun Koyama
Postgraduate Student Ai Murakami
Postgraduate Student Takayuki Oike
Postgraduate Student Toshitaka Sukisaki
Research Student
Chen meng
JICA Student Olivia Sebastian Rusizoka

Laboratory of biochemical research―１８―

HTLV-I-infected cells have been shown to have

Department of Preventive
Medicine and AIDS Researchhigh levels of active NF-κB and Tax has been demonstrated to active some cellular genes by causing an
increase in HF-κB levels. However, in the ATL sam-This department was newly added to the insti-

ples, viral mRAN are not detected. These results in-tute in １
９７
８ as a research division open to visiting

dicate that the celluar genes are constitutively over-professors from other universities and institutes. It

expressed in the absence of Tax in vivo. These find-is run by concurrent research staff for the present.

ings imply that there is another mechanism independ-

We have planned and started from January of１
９９７a

ent of Tax underlying the NF-κB activation in fresh

series of fundamental research to answer the ques-

ATL cells. The mechanism of Tax independent NF-

tion how and what mechanisms retroviral infection

κB activation is under investigation by using TL-

may lead to several diseases including acquired im-

Oml cells.

munodeficiency syndrome（AIDS）and adult T-cellleukemia （ATL）.

Study on mechanism of apotosis induction in HIV or
HTVL-I infection
Generally, the length of time between HIV infec-

Study on the mechanism of transactivation of severaltion and development of AIDS is considered to becellular genes by HTLV-I-Tax and HIV-Tat
HTLV-I and HIV are known to be causative

１０ years on average. HIV infection is accompanied

agents for ATL and AIDS, respectively. HTLV-I-

by the progressive loss of CD４ Tcells. Apoptosis, a

Tax and HIV-Tat are nuclear proteins which tran-

form of programmed cell death, has been implicatedscriptionally trans activate not only their own en-

in pathogenicity related to infection with HIV.

hancers in the long terminal repeat but also a number

HTLV-I Tax also leads to apoptotic cell death.

of cellular genes. We have previously demonstrated

Apoptotic pathway and its mechanism which accountthe capacity of the Tax of HTLV-I to modulate thefor the pathophysiology in HIV or HTLV-I infected

expression of various cellular genes: the cytokine

individuals are under investigation by using Tcells

genes for IL-１α, IL-６，IL-８，IL-１０，and the celltransfected to constitutively express Tat or Tax.

adhesion molecule gene for ICAM-１．We are now intending to study the mechanisms of transactivation ofVisiting Professor

Naoki Yamamoto

cellular genes including IL-８，IL-１０，ICAM-１，and

Visiting Associate Professor

Hironori SatoiNOS by Tax or Tat.

Technician

Masako Sasaki

Study on the mechanism of Tax independent NF-κB activation

Laboratory for biochemical research

Tissue culture room

―１
９―

experiment are performed to establish more realistic

Department of
Vector Ecology & Environment

larval indices. In the laboratory Aedes aegypti andAe. albopictus are studied on their ecological characters among geographical strains. Feeding behavior

Main interest of the department is analysis ofenvironmental factors that affect the trasmission of
insect-borne diseases, and pursuing environmental

and population dynamics of these mosquitoes also arestudied in the laboratory and fields.
３．Vector control technique related studies

friendly vector control strategy.

Basic study of copepods as a hopeful biological

１．Physiology and ecology of malaria vectors

control agent against vector mosquito larvae. Bio-

A long-term monitoring on main vectors of malaria has been conducted at several fields in South-

chemical approach to insecticide registance in vector
mosquitoes.

east Asian countries. Spatial and temporal changes
in larval habitats, vegetation, and the impact of hu-

Professor

Masahiro Takagi

man activity on the environment are evaluated in re-Assistant Professor

Yoshio Tsuda

lation to abundance of vectors, host preference, andResearch Associate

Nobuko Tuno

other population parameters. The GIS/RS is intro-

Research AssistantToshiko Ueno

duced as a new tool to analyze the environment. Geo-

Technician

Emiko Kawashimagraphical strains of several Anopheles are compara-

Technician

Yoshihide Maekawa

tively studied by morphological, physiological, andPostgraduate Student

Hamady Dieng

genetic approach.

Postgraduate Student

Tomomitsu Sato

２．Ecology and control of dengue vectors

Postgraduate Student

Maiko Hasegawa

Postgraduate Student

Susumu Saita

several fields in Southeast Asian countries. Monitor-

JICA Student

Hu Xi-Mining by oviposition traps and mark-release-recapture

JICA Student

Githuto Joyce Nyambura

Epidemiological studies have been conducted at

Stereomicroscopic observation of mosquitoes

Ramp traps for collecting mosquitoes―２０―Department ofSocial Environment
Specific Features of the Department
This Department covers interdisciplinary fieldof Environmental and Social Medicine related tohealth services and social welfare problems in developing countries.
Interdisciplinary approach includes studies in
Social Sciences and Humanities. Further, methods
and actual means of international cooperation toward
developing countries has been sought to utilize in order to promote inter-departmental linkage for information accumulated with specific analysis and professional response to the public particularly in thefield of infectious diseases diagnosis and control.
Under this context, basic and applied research
have been carried out in the following prospective
themes from the view point of environmental and social aspects for the purpose of promoting assistanceto minimize health problems in tropical areas:
Area of Research Activities:
１．Study and analysis on social (incl. life style, political and economical) background which regulates
frequency / areas / combination of diseases of thepresence of epidemics.
２．Study on the effect of individual and social environment toward endemic and epidemics.
３．Standardization of human security measures in
the field of health services from the view points ofnutrition and 3 Es ie.economy, environment andeducation.
４．Study on control of communicable diseases
among the regional and international health careprograms.
５．Study on quantitative and qualitative values of
medical and health services under the ODA ie. Official Development Assistance programs by Japa-

Dept. of Social and Environmental Medicine often
serves as a secretariat for Int’l Symposia.

nese government.
６．Feasibility study on comprehensive information
filing and network system for the sake of promoting health and social welfare services in developing countries, by epidemics, by regions, by administrative measures.
７．Reformation and coordination of health manpower traning program / system to meet the needof tropical area.
８．Comparative studies on the control of infectious
diseases in tropical Asia.
Specific themes of joint cooperative researchand research seminar designated to the Department
in FY 2002 under the scheme of Research Institutefor Nationally Joint-Use are listed below. The
Technical and Management Committee recommended
that interdisciplinary research collaboration should
be realized from the viewpoint of middle andlong-term range and managed not only by single
department but by the whole Institute as one of theimportant mandates.
Joint Research projects themes:a)Social and Environmental Diagnosis of Tropical
Diseases for its Control and Prevention.
b) Study on Application of Geographical Information
system in Tropical Diseases Controlc)Research on HIV / AIDS epidemic and its Control
in Developing Countries.
Research Seminar topic:
Tropical Diseases Control as Risk Management.
Professor
Research Associate
Research Associate
Research Assistant
Postgraduate Student
Research Student
JICA Student

Tsutomu Mizota
Susumu Tanimura
Eiko Kaneda
Eiko Tara
Chizuko Suzuki
Qin Liang
Susana Marta Borroto

Active chairmanship role creates interdisciplinary cooperative fruits.

―２
１―

(TNF-alpha promoter) alleles to cerebral malaria
２) Protective Immunity in falciparum malaria
HLA-Class I restricted CD 8 Tcell analysis in
the immunity

Department of
Molecular Immunogenetics
This department is focusing on the pathogenic
genetic factors of the host and the parasite in themost important tropical infectious diseases as follows.
１. Schistosoma japonicum
１) Pathogenesis of hepatosolenic disease
Immnopathology controlled by HLA-ClassII
genes is analysed by T cell epitope, Tcell cloning, tetramer analysis, Cytokine network analysis, ELISPOT analysis, 4 color FACS, animalmodel of HLA Tg mini-pig system
Identification of pathogenic genes by Multiple
families (MF) genetic analysis
Association analysis between disease and immune related polymorphic genes２) Molecular analysis of protective immunity in humans
Protective antigens effective for humans aresearched by checking reactivity of superresistant individuals in China.
Experimental animal model (pig)Analysis of protective immunity in pig, and Vaccine development targetedto somula stage antigens.
Estimation of mRNA expression in somula by
SAGE method
３) Surveillance system
Development of novel methodology for the longterm surveillance
Environmental research by GIS/RS is introduced to Hilly type schistosomiasis project
２. Malaria
１) Pathogenesis of Severe malaria
Functional analysis of the susceptible TNFP

３. Trypanosoma cruzi
１) Pathogenesis of the complications
Human genetic factors to develop Chagas heart,or the Mega disease by using HLA-class I,
MICA, B, TNFP, and other relevant genes.
Pathogenicity of the parasite
By Comparisons between three different pathogenic parasite lines derived from human patientswith different clinical types, cardiac, digestive,
and no complication.
Biological variety of the parasite lines molecular basis of the difference between intracellualr
proliferative (Peru 1,2) and non-proliferative,(H 1, H 23) are analysed by 2 D２) Protective immunity
Identification of a resistant gene
Analysis of the human T cell immunity
Protection from transplacental fetal infection.
４. Dengu virus
１) Pathogenesis of the DHF (Dengue Hemorragic
Fever)
Host factors will be detected by the Populational genetic analysis of the patients with DHF
and non DHF.
StaffsProfessor
Kenji Hirayama
Associate Professor Nobu Ohwatari
Assistant Professor Mihoko Kikuchi
Guest Research Fellow MOHAMED Raafat Taha
Technician
Junko Hayashima
Technician
Junko Kawashima
Postgraduate Student Ratawan Ubalee
JICA Student
Tran Phu Manh Sieu

Laboratory of Molecular Genetics

Clean Room for Cell Cuture―２２―

Department of Internal Medicine

Animal Research Center（University Hospital）
The department is one of the leading in the fieldof infectious diseases in the respiratory tract and infectious diseases in the tropical area. Besides lectures and training for medical students we make research on respiratory infections using quantitative
culture method with inflammatory cytology of the
sputum as well as bronchial secretes taken throughbronchofiberscope. Antibiotic concentration in clinical specimens and susceptibility of causative organisms to various drugs are also measured to evaluate
efficacy of chemotherapy. Clinical training for a doctor as a trainee is done to make a high quality physician, as not only a specialist for infectious field but ageneral physician for whole internal medicine.
Professor and Chief
Associate Professor
Assistant Professor
Research Associate
Research Associate
Research Associate
Research Fellow
Research Fellow
Research Fellow
Research Fellow
Research Fellow
Research Fellow
InternInternInternInternInternInternInternTsuyoshi Nagatake
Kazunori Oishi
Hiroshi Watanabe
Norichika Aso
Hiroyuki Yoshimine
Shouzaburo Onizuka
Takeshi Yamaryo
Konosuke Morimoto
Reiki Kuroki
Shinichi Kurita
Kazuhiko Hoshino
Kazuhiko Koyama
Yoshitaka Harada
Tsuyoshi Kuramoto
Reiko Mizutani
Masahiro Takaki
Takeshi Tanaka
Takahiro Nakama
Kei Matsuki

The center makes it the principal aim to ensure
the safety of animal experiments which deal with thepathogenic microorganisms and to build up the successful generation of experimental animals, microorganisms and parasites. The building consists of 7
breeding rooms of experimental animals, 2 laboratories, one breeding room of snails, one insectarium,
two P 3-level biohazard laboratory and breedingroom. The air pressure is kept to be always negative
to avoid outflow from inside even at entrance tobuilding. Since the building has the most thoroughventilation through HEPA filter, any microbe cannot
leak out to outside of the building. The water used isgiven chlorination and drained off. The breeding animals and experiments are done according to the
Guideline for Animal Experiment edited by Nagasaki
University. The laboratory animals bred in the center are mice, rats, hamsters, gerbils, rabbits, snails
and mosquitoes.
Professor and Director
Michio Nakamura
Research Associate
Tetsuo Yanagi
Senior Research Assistant Mariko Kozato

Mongolian gerbil （ Meriones unguicalatus ）Clinical conference―２
３―poisonous marine animals.

Research Center for Tropical
Infectious Diseases

The center carries out eco-epidemiologicalstudies on tropical diseases by means of fieldwork at
the actual site of the problem. We also use computer

The Research Center for Tropical Infectious

science technology, such as the geographical infor-

Diseases, Which Inherited its history from the infor-

mation system, remote sensing and information ex-

mation and Reference Center of Tropical Medicine,changing system, which project the future of tropicalwas established in April 2001. According to the his-

infectious diseases from both local and global per-tory the center has three missions.

spective. One of our main interest is the role of hu-

The center functions as a museum of tropicalman behavior in the transmission of tropical infec-

diseases, which is unique in Japan. Next, the centertious diseases. Taxonomy and ecology of vector in-also provides necessary information and references

sects and mites of infectious diseases are also the re-related to tropical diseases to all the staff, graduate

search subjects of the center.

students, researchers and trainees, as well as members of the public who visit the Institute. The third

Professor

Masaaki Shimada

mission of the center is to contribute to the global

Professor

Kazuhiko Mojicontrol of the tropical infectious diseases.

We

Associate Professor

Hiroshi Suzuki

analyze the factors that regulate the epidemics of theAssistant Professor

Yuji Ataka

diseases to find appropriate control measure of the

Research AssistantToshihumi Oyama

infection.

Research Assistant

Kyoko Sakitani

Technician

Kiyomi Suda

References currently available at the center are

Graduate Student

Tomoko Kisu

books, maps, periodicals, pamphlets, films, videocas-

Graduate Student

Yuko Nakao

sette tapes, photo slides, photographs, charts, exhi-

Graduate Student

Akio Ohno

bition panels, animal specimens as well as pathologi-

JICA Student

Mingu David Mathu

cal tissue specimens. These materials are now being
digitized and are provided to the public through cyberspace. The center also gathers scientific, social
economic, and cultural information related to tropical diseases. Our current exhibitions consist of panels of major tropical diseases, diarrhea diseases,
acute infectious diseases, AIDS related diseases,
and specimen of parasites, vector arthropods andExhibition room

Schistosomiasis transmission site
(Collection of vector snails)―２
４―Central Laboratory

Administration

There are several equipments at this laboratory.

Yoji Shimizu, Head Official

It is open for collaboration research with all part ofGeneral Affairs Unit

Japan followings are the equipments now available.
Electron microscopies (TEM and SEM of jeol), a Ultramicrotome( Reichert ) ,

a

flow

cytometer(FACScan), a cell sorter (FACSsatar plus), scanning

Rikio Yoshidomi, Chief
Hidemitsu Baba, Sub-Chief
Nanami Tsuji, Assistant Staff

electron microscopies (Bio rad MRC 600 and Zeiss
LSM), micromanipulation system (Nikon-Narishige
and Zeiss-shimazu), Bioimage analizers (Hamamatsu
Pheotonics, Bio rad GS-250 and Pharmacia Image
master), a peptide synthesizer (Millipore 600 E), a
peptide sequencer (Shimazu PPSQ-10), DNA sequancers(Perkin-Elmer 373-70

andAccounting Unit
Tsukasa Urakawa, Chief
Tsukasa Harada,Staff
Kouzou Aota, Staff
Yumiko Yamada, Assistant Staff
Asuka Matsuo, Assistant Staff

Pharmacia

AFL), a DNA/RNA extraction system (Perkin-

Collaboration Research Unit

Elmer 341-30), a real-time surface plasmon detecter

Keiko Matsuda, Chief(Fisons IAsys), two P 3 rooms, Super sentrifugal

Narumi Sasaki, Sub-Chiefmachine (optima L-90 K). with other equipments, ex-

Junko Suenaga, Assistant Staff

periments on cell biology and histochemistry can be
carried out.

Professor and Chief

Toshiya Hirayama

Research Associate

Akitoyo Ichinose

Research Assistant

Kumi Tamura

The SEM of electron microscope Laboratory

Administration office―２
５―

Location map of the Institute ofTropical Medicine on SakamotoCampus of Nagasaki University

PlaygroundGymnasium

Laboratory Animal Center
for Biomedical Research

Parking

Radioisotope
CenterMedical Materials
Center for Atomic
Bomb Casuality
Research Center for
Tropical Infectious Diseases
In part of the
3rd and 4th Floor

Sub-Gate()

Main Building of
the Institute ofTropical Medicine
School of Medicine
AtomicDiseaseInstitute

Animal Research Center

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ン
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館

Main Gate
MedicalBranchLibrary
AlumniHall

Commemoration
Hall

Pompe
Hall

Back GateN

for University Hospital―２
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Telephone Number
Institute of Tropical Medicine, Nagasaki University ………………………………………０
９５（８４９）７８００
Extensions
Dean ………………………………………………………………………………………４７００

８４９‐７８０１

Head of Administrative Office ……………………………………………………………４７０１

８４９‐７８０２

Chief of General Affairs Unit ……………………………………………………………４７０２
General Affairs Unit ………………………………………………………………………４７０３

８４９‐７８０３

Facsimile …………………………………………………………………………………４７０５

８４９‐７８０５

Chief of Accounting Unit …………………………………………………………………４７０６
Accounting Unit …………………………………………………………………………４７０７

８４９‐７８０６

Accounting Unit …………………………………………………………………………４
７０８
Chief of Collaboration Research Unit ……………………………………………………４７０９
Collaboration Research Unit ………………………………………………………………４７１０

８４９‐７８０７

Meeting Room ………………………………………………………………………… ４
７１１
Department of Virology
Professor …………………………………………………………………………４７３３

８４９‐７８２７

Associate Professor ………………………………………………………………４７３４

８４９‐７８２８

Information ………………………………………………………………………４７３５

８４９‐７８２９Facsimile …………………………………………………………………………４７３６

８４９‐７８３０

Department of Bacteriology
Professor …………………………………………………………………………４７３７

８４９‐７８３１

Lab．
９ ……………………………………………………………………………４７３８

８４９‐７８３２

Information ………………………………………………………………………４７３９８４９‐７８３３

Department of Protozoology
Professor …………………………………………………………………………４７４１

８４９‐７８３５

Associate Professor ………………………………………………………………４７４２

８４９‐７８３６

Lab．
３ ……………………………………………………………………………４７４３

８４９‐７８３７

Information ………………………………………………………………………４７４４

８４９‐７８３８Department of Parasitology
Professor………………………………………………………………………４７２８

８４９‐７８２２

Associate Professor……………………………………………………………４７２９８４９‐７８２３

Research Associate……………………………………………………………４７３０

８４９‐７８２４……………………………………………………………………４７３１

８４９‐７８２５

Information

Department of Molecular Epidemiology
Professor………………………………………………………………………４７７０

８４９‐７８６０

Department of Thermal Adaptation
Professor （Concurrent）…………………………………………………………４７２６

８４９‐７８２０

Department of Biochemistry
Professor …………………………………………………………………………４７５４

８４９‐７８４８

Lab．
１ ……………………………………………………………………………４７５５

８４９‐７８４９Lab．
２ ……………………………………………………………………………４７５６

８４９‐７８５０

Information ………………………………………………………………………４７５７

８４９‐７８５１―２
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Department of Pathology

Extensions

Professor …………………………………………………………………………４７１９８４９-７８１３

Associate Professor ………………………………………………………………４
７２０

８４９-７８１４

Lab．
２ ……………………………………………………………………………４７２１

８４９-７８１５

Information ………………………………………………………………………４７２２

８４９-７８１６

Department of Internal Medicine
Professor …………………………………………………………………………４７４６

８４９-７８４０

Associate Professor ………………………………………………………………４７４７

８４９-７８４１

Information ………………………………………………………………………４７４８

８４９-７８４２

Facsimile …………………………………………………………………………４７４９８４９-７８４３

Department of Preventive Medicine and AIDS Research
Professor …………………………………………………………………………４７５０

８４９-７８４４

Research Associate ………………………………………………………………４７５１

８４９-７８４５

Information ………………………………………………………………………４７５２

８４９-７８４６

Department of Vector Ecology & Environment
professor …………………………………………………………………………４７１６

８４９-７８１０

Staff room ………………………………………………………………………４７１７

８４９-７８１１

Information ………………………………………………………………………４７１５

８４９-７８０９

Facsimile …………………………………………………………………………４７１８

８４９-７８１２

Department of Social Environment
Professor …………………………………………………………………………４７７４

８４９-７８６４

Information ………………………………………………………………………４７７５

８４９-７８６５

Information ………………………………………………………………………４７７６

８４９-７８６６

Facsimile …………………………………………………………………………４７７７

８４９-７８６７

Department of Environmental Pyhsiology
Professor …………………………………………………………………………４７２４

８４９-７８１８

Associate Professor ………………………………………………………………４７２５

８４９-７８１９

Information ………………………………………………………………………４７２６

８４９-７８２０

Facsimile …………………………………………………………………………４７２７

８４９-７８２１

Animal Research Center
Information ………………………………………………………………………４７６２

８４９-７８５６

Laboratory ………………………………………………………………………４７６３

８４９-７８５７

Research Center for Tropical Infectious Diseases
Professor …………………………………………………………………………４７７８

８４９-７８６８

Professor …………………………………………………………………………４７１４

８４９-７８０８

Associate Professor ………………………………………………………………４７５９

８４９-７８５３

Computer Room! ………………………………………………………………４７７８

８４９-７８６８

Information ………………………………………………………………………４７６０

８４９-７８５４

Facsimile …………………………………………………………………………４７７９

８４９-７８６９

Central Laboratory
Electron Microscope Room ………………………………………………………４
７６５
Computer Room …………………………………………………………………４７６６
FACS Room………………………………………………………………………４７６７
Section for Experimental Animals ………………………………………………４７６９―２
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８４９-７８５９

Location map of the Institute of Tropical Medicine,
Nagasaki University in Nagasaki City

Mt. Inasa

Ｎ

Ropeway

Nagasaki Bay

Urakami

fo

rK

og

ak

for Akasako−
OhashiuraHamaguchi-machi
Urakamieki-mae
Nagasaki−
Ohato

Igakubu-mae

Nagasakieki-mae

School of
Dentistry
University
Hospital

School ofMedicine

Urakami−tenshudo-mae

Isibashi
Tsukimachi

Prefectural OfficeInstitute of Tropical
Medicine

City Hall−−
Chuobashi−−
Kokaido-mae
Suwajinja-mae−
Shokakuji-shita

JR Kyushu
Streetcar
Main bus routfor Mogi

for Hotarujaya

How to get the Institute
１．From Nagasaki Station （JR Kyushu）!Get on a bus (Nagasaki Bus, Line ８）at Nagasakieki-mae and get off at Igakubu-mae.
Five minutes walk from the bus stop.
"Get on a streetcar at Nagasakieki-mae（for Akasako, Line １or３）, and get off at
Hamaguchi-machi. Ten minutes walk from the streetcar stop.
#Ten minutes by taxi from the station.
２．From Urakami Station（JR Kyushu）!Twenty minutes walk.
"Five minutes taxi.
３．From Nagasaki Airport!Get on limousine at the airport（for Nagasaki）, and get off at Nagasaki-ekimae, andthen continue to１．
"Get on limousine at the airport（for Nagasaki）, and get off at Ohashi, and then twenty
minutes walk from the bus stop or ５ minutes by taxi.
#About one hour by taxi from the airport.
Location
１‐
１２‐４ Sakamoto Nagasaki ８５２‐
８５２３
URL http://www.tm.nagasaki-u.ac.jp

Published on May３
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Edited by the Information and Reference Center of Tropical Medicine, Institute of Tropical Medicine,
Nagasaki University
Printed by Showado Printing Co.,１００７‐２ Nagano machi, Isahaya８
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６，Japan