MMS 研究会_共同研究レポート＜共同研究 6＞
研究題名： 小核試験における AO 超生体染色法の検証
研究成果： Hayashi らによって開発されたアクリジンオレンジを用いる超生体染色を用いる小核試験の性能を検証した．試験の手法が簡単であることもあり，期待どおりの結果が，安定していられた．また，標本作製時期も験体投与後 48 時間でほとんどの化学物質の小核誘発性を検出することが出来た．
発表論文：
1)

The Collaborative Study Group for the Micronucleus Test. Micronucleus test with mouse peripheral blood erythrocytes byacridine orange supravital staining: the summary report of the 5th collaborative study by CSGMT/JEMS.MMS. Mutat Res.
1992 Feb-Mar;278(2-3):83-98.

2)

Hatanaka Y, Kitagawa Y, Toyoda Y, Kawata T, Ando N, Kawabata Y, Iwai M, Arimura H. Micronucleus test withcyclophosphamide using mouse peripheral blood reticulocytes, Mutat Res. 1992, 278, 99-101.

3)

Sato S, Taketomi M, Morita T. Simplified mouse peripheral reticulocyte micronucleus test with dimethylnitrosamine, Mutat
Res. 1992, 278, 103-107.

4)

Kondo K, Ozawa S. Micronucleus test with ethyl methanesulfonate in mouse peripheral blood reticulocytes stained
supravitally using acridine orange-coated slides, Mutat Res. 1992, 278, 109-111.

5)

Hitotsumachi S, Kimura Y, Katoh M, Ishihara N, Hara T, Shibuya T. Micronucleus tests on N-ethyl-N-nitrosourea with mouseperipheral blood reticulocytes using acridine orange-coated slides, Mutat Res. 1992, 278, 113-115.

6)

Sugiyama C, Miyamae Y, Kobayashi H, Fujino Y, Mori M, Ohara K. The micronucleus test of methyl methanesulfonate withmouse peripheral blood reticulocytes using acridine orange-coated slides, Mutat Res. 1992, 278, 117-120.

7)

Kuramochi M, Seki H, Tazawa T, Sakai S, Sakai Y. The micronucleus test with mouse peripheral blood on N-methyl-N'-nitroN-nitrosoguanidine and mitomycin C, Mutat Res. 1992, 278, 121-125.

8)

Yamamura E, Hirono H, Takeuchi M, Kojima M, Aoki S. The micronucleus assay with mouse peripheral blood reticulocytes
using acridine orange-coated slides with triethylenemelamine, Mutat Res. 1992, 278, 127-130.
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9)

Iwakura K, Tamura H, Matsumoto A, Ajimi S, Ogura S, Kakimoto K, Matsumoto T, Hayashi M. The micronucleus assay withperipheral blood reticulocytes by acridine orange supravital staining with 1-β-d-arabinofuranosylcytosine, Mutat Res. 1992,278, 131-137.

10) Ohuchida A, Furukawa A, Yoshida J, Watanabe M, Aruga F, Miwa Y, Shinkawa K, Kinae N. Micronucleus assays on 5fluorouracil and 6-mercaptopurine with mouse peripheral blood reticulocytes, Mutat Res. 1992, 278, 139-143.
11) Kasahara Y, Wakata A, Nakai Y, Yuno K, Miura D, Yagi K, Hirabayashi K, Makita T. The micronucleus test using peripheral
blood reticulocytes from methotrexate-treated mice, Mutat Res. 1992, 278, 145-151.
12) Asano No, Hagiwara T. The mouse peripheral blood micronucleus test with 2-acetylaminofluorene using the acridine orange
supravital staining method, Mutat Res. 1992, 278, 153-157.
13) Higashikuni N, Baba T, Nakamura T, Sutou S. The micronucleus test with peripheral reticulocytes from phenacetin-treatedmice, Mutat Res. 1992, 278, 159-164.
14) Shimada H, Suzuki H, Itoh S, Hattori C, Matsuura Y, Tada S, Watanabe C. The micronucleus test of benzo[a]pyrene withmouse and rat peripheral blood reticulocytes, Mutat Res. 1992, 278, 165-168.
15) Suzuki T, Tamai K, Kodama Y, Asita AO, Matsuoka A, Sofuni T, Kurita M, Ohtsuki H, Hiwatashi T, Hayashi M. Micronucleus
induction in mouse peripheral reticulocytes by 7,12-dimethylbenz[a]anthracene, Mutat Res. 1992, 278, 169-173.
16) Hara M, Nakagawa S, Fujioka E, Ayukawa E, Izushi T. Detection of micronuclei in peripheral blood of mitomycin C-treatedmice using supravital staining with acridine orange, Mutat Res. 1992, 278, 175-179.
17) Awogi T, Murata K, Uejima M, Kuwahara T, Asanami S, Shimono K, Morita T. Induction of micronucleated reticulocytes bypotassium bromate and potassium chromate in CD-1 male mice, Mutat Res. 1992, 278, 181-185.
18) Kondo Y, Honda S, Nakajima M, Miyahana K, Hayashi M, Shinagawa Y, Sato S, Inoue K, Nito S, Fumio A. Micronucleus testwith vincristine sulfate and colchicine in peripheral blood reticulocytes of mice using acridine orange supravital staining,Mutat Res. 1992, 278, 187-191.
19) Hatakeyama Y, Nakajima E, Atai H, Suzuki S. Effects of benzene in a micronucleus test on peripheral blood utilizing acridine2MMS 研究会_共同研究レポート

orange-coated slides, Mutat Res. 1992, 278, 193-195.
20) Romagna F, Matsumura H, Watanabe M, Kato T, Shirasu Y, Ohmori K, Yamada H, Sasaki YF. Micronucleus evaluation inperipheral blood reticulocytes of mice treated with procarbazine hydrochloride or mitomycin C, Mutat Res. 1992, 278, 197204.
21) Kishi M, Horiguchi Y, Watanabe S, Hayashi M. Validation of the mouse peripheral blood micronucleus assay using acridine
orange supravital staining with urethane, Mutat Res. 1992, 278, 205-208.
22) Hayashi M, Kodama Y, Awogi T, Suzuki T, Asita AO, Sofuni T. The micronucleus assay using peripheral blood reticulocytes
from mitomycin C- and cyclophosphamide-treated rats, Mutat Res. 1992, 278, 209-213.

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