Location map of the Institute of Tropical Medicine,
Nagasaki University in Nagasaki City

INSTITUTE OF TROPICAL MEDICINENAGASAKI UNIVERSITY

Mt. Inasa

Ｎ

Ropeway

Nagasaki Bay

Urakami

fo

rK

ak

for Akasako−
OhashiuraHamaguchi-machi
Urakamieki-mae
Nagasaki−
Ohato

Igakubu-me

Nagasakieki-mae

School of
Dentistry
University
Hospital

School ofMedicine

Urakami−tenshudo-mae

Isibashi
Tsukimachi

Prefectural OfficeInstitute of Tropical
Medicine

City Hall−−
Chuobashi−−
Kokaido-mae
Suwajinja-mae−
Shokakuji-shita

JR Kyushu
Streetcar
Main bus routfor Mogi

for Hotarujaya

How to get the Institute
１．From Nagasaki Station （JR Kyushu）
Get on a bus (Nagasaki Bus, Line ８）at Nagasakieki-mae and get off at Igakubu-mae.
Five minutes walk from the bus stop.
Get on a streetcar at Nagasakieki-mae（for Akasako, Line １or３）, and get off at
Hamaguchi-machi. Ten minutes walk from the streetcar stop.
Ten minutes by taxi from the station.
２．From Urakami Station（JR Kyushu）
Twenty minutes walk.
Five minutes taxi.
３．From Nagasaki Airport
Get on limousine at the airport（for Nagasaki）, and get off at Nagasaki-ekimae, andthen continue to１．
Get on limousine at the airport（for Nagasaki）, and get off at Ohashi, and then twenty
minutes walk from the bus stop or ５ minutes by taxi.
About one hour by taxi from the airport.
Location
１‐
１
２‐４ Sakamoto Nagasaki ８５２‐
８５２３
URL http : //www.tm.nagasaki-u.ac.jp

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熱研案内パンフ（英文）＊ＣＭＹＫASFOU ND E D 1 9 42

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SEPTEMBER

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Published on September ８，２０００Edited by the Information and Reference Center of Tropical Medicine, Institute of Tropical Medicine,
Nagasaki University
Printed by Showado Printing Co.,１００７‐２ Nagano machi, Isahaya８
５
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MISSION STATEMENT
Institute of Tropical Medicine, Nagasaki University

The tropics, the most ecologically diverse region on the Earth, presents an ongoing complexity of tropicaldiseases and other health problems. In view of the remarkable advances made in the field of international exchange in recent years, it is imperative that these problems be addressed from a global perspective.
Based on this understanding, the Institute of Tropical Medicine, Nagasaki University, aims to overcome
tropical diseases, particularly infectious diseases, and the various health problems associated with them, in cooperation with related institutions, to strive for excellence in the following areas :１．Spear-head research in tropical medicine and international health
２．Global contribution through disease control and health promotion in the tropics by applying the
fruits of the research
３．Cultivation of the researchers and specialists in the above fields

General view of the InstitutePreface
The Institute of Tropical Medicine, Nagasaki University, established in
１９４
２，
is a unique government-supported institution for the research on tropical
medicine, both in the basic and applied fields. Its reorganization led to the firstcollaborative institute in medical science in Japan in１９８９，and designation as
one of the Centers of Excellence in１
９９５．Present organization of the instituteis composed of３major research fields（１１departments，
１domestic visiting
department，
１overseas visiting department），２centers, and１clinical unit.
In November１９９６，the１４th International Congress for Tropical Medicine
and Malaria was held in Nagasaki, presided by Prof. Emeritus Keizo Matsumoto, with active participation by
the staffs of the institute, resulting in the globalization of the institute and its staffs. On this occasion, a memorandum of collaborative research was signed between the institute and the National Institute of Allergy and Infectious Diseases/National Institute of Health. the US.
According to the first external review in１９９６，the institute worked out its Mission Statement as shown on
a separate page. In１
９９８，the Reference Center of the institute was reorganized into Information and ReferenceCenter for Tropical Diseases, which is responsible to provide various information on tropical medicine, in addition to its previous activities. During the Education and Culture Week in１９９７，special exhibition of the Center was carried out in order to improve the understanding on the institute and tropical medicine by the societies.
Follow-up of the participants in the tropical medicine training course was carried out on its２
０th Anniversary in１
９９
７．Since a similar course operated at the Institute of Medical Science, the University of Tokyo wasclosed in１
９９９，the course in our institute became the sole training course on tropical medicine in Japan, operating with１
５participants in２
０００．The number of applicant has been in excess to the number of admitted participants during the past several years. Many of them have already been to some tropical areas, and were motivated for further studies through their own experiences.
Another group training course "Research on Tropical Medicine" sponsored by JICA was significantly
strengthened in１
９９７，by extending its period to１
２months, inviting participants nearly from１
０countries. For
such activities contributing to the friendship with foreign countries, the institute was officially commended by
the Minister of Foreign Affairs of Japan in July１
９９８．The title of the certificate endowed to the participantsin the group training course was changed from previous "Certificate" to "Diploma".
In the year２０００，the exchange program under the core university system by JSPS was newly establishedutilizing our institute and National Institute of Hygiene and Epidemiology as the core university in Japan and
Vietnam, respectively. This project has drawn much attention by the external scientists as an example of future
project type research scheme in which researchers from multiple departments can participate regardless of
their different speciality.
Memorandom of academic exchange program was signed between Nagasaki university and Mahidol university, Bangkok, Thailand.
In the year１９９
９，JICA newly established "Developmental Partner Project", and our Malaria Control Project was among subjects approved by the JICA. This would be another overseas research project of the institute. Besides, International Program of Parasitic Diseases. so-called Hashimoto Initiatives, has been participated by a number of investigators of this institute.
According to the current reformation policy of Japanese government, all national university in Japan arescheduled to be independent organizations. This future outlook should be accepted as de facto, followed by appropriate responses. The Institute of Tropical Medicine, Nagasaki University, is also not an exception, and isrequired to effectively concentrate its resources to appropriate subjects along the line shown in its Mission
Statement, towards international contribution in the field of tropical medicine and international health.
Kind and generous support to the institute is highly appreciated.
１１August２
０００
Akira Igarashi, MD, PhD
Professor and Dean
Institute of Tropical Medicine
Nagasaki University―１―

Contents
Preface…………………………………………………………………………………………１

Contents…………………………………………………………………………………………２

Historical Review………………………………………………………………………………３

Successive Deans of the Institute……………………………………………………………４

Organizational Chart…………………………………………………………………………５

Collaboration Research………………………………………………………………………６

Institute of Tropical Medicine Steering Committee………………………………………７

Institute of Tropical Medicine Collaboration Professional Committee
Scope of Activity………………………………………………………………………………９

Postgraduate School…………………………………………………………………………９Three-month Course on Tropical Medicine and Related Studies
Training Course in Research of Tropical Medicine
Public Lectures at the InstitutePublications…………………８…………………………９…………………………………………１
０………………………………………………………………１
０……………………………………………………………………………………１
０

Department of Virology………………………………………………………………………１
１

Department of Bacteriology…………………………………………………………………１
２

Department of Protozoology…………………………………………………………………１
３

Department of Parasitology…………………………………………………………………１
４

Department of Thermal Adaptation
Department of Biochemistry
Department of Pathology…………………………………………………………１
５…………………………………………………………………１
６………………………………………………………………………１
７

Department of Internal Medicine……………………………………………………………１
８

Department of Preventive Medicine and AIDS Research…………………………………１
９

Department of Medical Entomology…………………………………………………………２
０

Department of Social Environment…………………………………………………………２
１

Department of Environmental Physiology……………………………………………………２
２

Department of Internal Medicine（University Hospital）
Animal Research Center………………………………………………………………………２
３

Information and Reference Center of Tropical Medicine
Central Laboratory
Library…………………………………２３…………………………………２４……………………………………………………………………………２４…………………………………………………………………………………………２
５

Administration…………………………………………………………………………………２
５

Location map of the Institute of Tropical Medicine on Sakamoto Campusof Nagasaki University…………………………………………………………………………２
６

Ground plan of the Institute, 1st Floor, 2nd Floor…………………………………………２
７

Ground plan of the Institute, 3rd Floor, 4th Floor…………………………………………２
８Ground plan of the Animal Research Center
Telephone Number…………………………………………………２
９……………………………………………………………………………３
０―２―

Historical Review
The Institute of Tropical Medicine, Nagasaki
University was originally founded in March 1942, as
the East Asian Research Institute of Endemics, inorder to perform basic and applied studies on endemic diseases in East Asia. At the beginning, mostof its research activities were field studies in mainland China, performed by several department such as
Pathology, Bacteriology, Internal Medicine and Dermatology of Nagasaki College of Medicine. Unfortunately, all the facilities and research materials werecompletely destroyed instantaneously along with theMedical School by the atomic bomb which explodedon August 9th, 1945. As a result, development of theinstitute and its research activities were severely inhibited.
In April 1946, the institute was named as the
Research Institute of Endemics attached to Nagasaki
College of Medicine, and moved to Isahaya City in
May in order to resume its research activities. In accordance with the Act on the Foundation of National
Schools in May 1949, the institute was renamed as
the Research Institute of Endemics, Nagasaki University. Becauce of the severe flood in Isahaya City,
the construction of the new building in Sakamotomachi, Nagasaki City was started in 1960, and the institute moved to new building in April, 1961. At thattime, there were only two departments, Pathology
and Clinics, however, since 1964, new departments
were established every Year, such as Epidemiology,
Parasitology, Virology and at the end of 1966, thefirst extension of the building was completed.
In June 1967, according to the partial alterationof the Act on the Foundation of National Schools, thename of the institute was changed to the present one,

Animal Research Centerin order to perform basic as well as applied studies
on tropical medicine. At the same time, the Department of Internal Medicine of the institute with 20 bed
facilities was opened in the University Hospital. In
1974, Department of Bacteriology and ReferenceCenter as an attached facility were opened. In 1978,
the Department of Preventive Medicine suppoeted byvisiting staff and the Training Course of Tropical
Medicine were started. In 1979, Ward of Infectious
animals became Animal Research Center. In March
1980, the 2nd extension of the main building was concluded. In September 1983, the Training Course in
Research for Tropical Medicine by JICA was opened.
In 1984,Department of Protozoology was established.
In July 1985, the 3rd extension of the building wascompleted. In 1987, Department of Medical Entomology was established. In 1989, the institute wasreorganized to a collaboration research institute. In
1991, Department of Biochemistry was added. In
March 1994, the 4th extension of the building wascompleted, and in April, 1994, the institute was reorganized to 3 research fields, Tropical Microbiology,
Pathogenesis and Clinical Sciences, and Environmental Medicine, with addition of 2 new research departments, Thermal Adaptation and Social Environment, and the institute has 12 research departments
at present. In 1995, the Institute was designated as
one of the "Centers of Excellence" in the forefront ofscientific research. In 1996, a new research department, Molecular Epidemiology, was established under the Research Field of Microbiology to invite anoverseas visiting professor. In 1997, the ReferenceCenter was abolished and in its place the Information
and Reference Center of Tropical Medicine was established, symbolizing continuous consolidation andre-organization of the Institute.

The ３rd and ４th extension building including Information and Reference Centerof Tropical Medicine, The Institute of Tropical Medicine, Nagasaki University―３―

Successive Deans of the Institute（East Asian Research Institute of Endemics）
Susumu Tsunoo

May．
４，
１
９
４
２-Aug．
２
２，
１
９
４
５

Kohei Koyano

Dec．
２
２，
１
９
４
５-Jan．
２
３，
１
９
４
８

Kiyoshi Takase

Jan．
２
４，
１
９
４
８-Aug．
３
１，
１
９
４
８

Noboru Tokura

Sept．
１，
１
９
４
８-May．
３
０，
１
９
４
９

（Research Institute of Endemics）
Noboru Tokura

May．
３
１，
１
９
４
９-Aug．
３
１，
１
９
５
８

Nanzaburo Omori

Sept．
１，
１
９
５
８-Nov．
３
０，
１
９
６
３

Hideo Fukumi

Dec．
１，
１
９
６
３-May．
３
１，
１
９
６
７

（Institute of Tropical Medicine）
Hideo Fukumi

June．
１，
１
９
６
７-Nov．
３
０，
１
９
６
９

Daisuke Katamine

Dec．
１，
１
９
６
９-Nov．
３
０，
１
９
７
３

Kaoru Hayashi

Dec．
１，
１
９
７
３-Nov．
３
０，
１
９
７
７

Tatsuro Naito

Dec．
１，
１
９
７
７-Nov．
３
０，
１
９
７
９

Daisuke Katamine

Dec．
１，
１
９
７
９-Apr．
１，
１
９
８
１

Keizo Matsumoto

Apr．
２，
１
９
８
１-Apr．
１，１
９９１

Hideo Itakura

Apr．
２，
１
９
９
１-Apr．
１，
１
９
９３

Mitsuo Kosaka

Apr．
２，
１
９
９
３-Apr．
１，
１
９
９
７

Akira Igarashi

Apr．
２，
１
９
９
７-Up to the present―４―Organizational Chart
Department of Virology
Department of Bacteriology
Research Field ofMicrobiology

Department of Protozoology
Department of Parasitology
Department of Molecular Epidemiology(Overseas Visiting Professor)
Department of Thermal Adaptation
Department of Biochemistry

Research Field ofPathogenesis
and Clinical
Sciences

Department of Pathology
Department of Internal Medicine
Department of Preventive Medicine
and AIDS Research
Department of Medical Entomology

Dean

Faculty
Meeting

Research Field ofEnvironmental
Medicine

Department of Social Environment
Department of Environmental Physiology

Attached
Facilities

Animal Research Center
Information and Reference Centerof Tropical Medicine
Central Laboratory
Library
General Affairs Unit

Administration

Accounting Unit
Collaboration Research Unit

University
Hospital

Clinical
Department

Department of Internal Medicine―５―These activities of the institute are expected toCollaboration researchcontribute to remarkable progress of tropical medicine.

The institute has conducted research in the field

The senior staff composed of professors of theof tropical medicine for the past ６
０ years since its

institute and the executive committee make plans forestablishment in１
９４
２．The institute was reorganizedresearch work. The steering committee and the col-to make extensive collaboration with other universi-

laboration professional committee composed of expe-

ties and institute in the field of interdisciplinary

rienced scientists coordinate the various collabora-

tropical medicine with molecular biology, entomology,tion researchs.

anthropology, social medicine, etc.

Collaboration Research Sytem

Steering Committee

The senior
staff（Professors）

Collaboration professional
committee

Executive committee―６―

Institute of Tropical Medicine Steering Committee
The University of Tokyo
National Institute of Infectious
Diseases
Nagasaki University

Professor Emeritus
Honorary Post
Professor Emeritus

Manabu Sassa
Akira Oya
Keizo Matsumoto

The Institute of Medical Science
The University of Tokyo

Director

Ken-ichi Arai

Research Institute for Microbial
Diseases Osaka University

Director

Yoshitake Nishimune

The International Medical Center

President

Yoshio Yazaki

National Institute of Infectious
Diseases

Director

Yoshifumi Takeda

Japan International Cooporationof Welfare Services

Senior Medical Adviser

Takashi Wagatsuma

Kobe University

Professor

Shigeaki Sato

Professor

Keiji Ide

Nagasaki University
Faculty of Economics
School of Medicine

Dean

Hiroshi Saitou

Institute of Tropical Medicine

Dean◎Akira Igarashi

〃
〃
〃
〃
〃
〃
〃
〃
〃
〃

Professor

Toshiya Hirayama

Professor

Hiroji Kanbara

Professor

Yoshiki Aoki

Professor

Michio Nakamura

Professor

Hideyo Itakura

Professor

Tsuyoshi Nagatake

Professor

Naoki Yamamoto

Professor

Masahiro Takagi

Professor

Tsutomu Mizota

Professor

Masaki Shimada◎：Chairman―７―Institute of Tropical Medicine Collaboration Professoional Committee
Gunma University

Professor

Mamoru Suzuki

Kyusyu University

Professor Emeritus

Isao Tada

Kobe University

Professor

Haku Hotta

Kumamoto University

Professor

Hiroshi Maeda

University of the Ryukyus

Professor

Shigeo Nonaka

Oita Medical College

Professor

Kumato Mifune

Miyazaki Medical College

Professor

Youichi Minamishima

University of Occupational andEnvironmental HealthProfessor

Takekiyo Yoshimura

Osaka International University

Professor

Yuji Yamamoto

Keio University

Professor

Yoshiyasu Takefuji

School of Medicine

Professor

Taiichirou Takemoto〃

Professor

Shigeru Katamine

School of Pharmaceutical Sciences

Professor

Nobuyuki Kobayashi

Institute of Tropical Medicine

Professor◎Hideyo Itakura〃

Professor

Akira Igarashi〃

Professor

Toshiya Hirayama〃

Professor

Hiroji Kanbara〃

Professor

Yoshiki Aoki〃

Professor

Michio Nakamura〃

Professor

Tsuyoshi Nagatake〃

Professor

Naoki Yamamoto〃

Professor

Masahiro Takagi〃

Professor

Tsutomu Mizota〃

Professor

Masaaki Shimada〃

Assistant
Professor

Nobu Oowatari

Nagasaki University◎：Chairman―８―

Scope of Activity

Postgraduate School

Based upon the mission statement issued in１
９９
９，

The Docter Course in the Institute belongs toin the Institute of Tropical Medicine, basic and ap-

the Postgraduate School of Medical Science in Na-

plied studies on tropical medicine are being under-

gasaki University. Students who want to study in thetaken. These studies aim at developing knowledge for

Course must pass the entrance examinatoin of thediagnosis, treatment and prevention of tropical dis-

School and thereafter can select the special subject

eases focusing on infectious ones.

listed by each professor in the Institute. The number

The research activities consists of three major
fields ; research on pathogens such as viruses, bac-of the student in ２
０００ is ３１（including foreign students）.

teria, protozoa and helminthes, research on humanpathology and research on natural and social environ-Three-month Course on Tropical
Medicine and Related Studies

ment. Research subjects are of wide range from thebench to the latrine, from molecular biology to fieldsurveys at grassroot level. Collaborative studies
with World Health Organization, Ministry of Foreign

The course aims to provide participating per-Affairs of Japan, Japan International Cooperation

sons with a better understanding of the health prob-

Agency, foreign universities and institutions are onlems in tropics, to increase their ability to cope withgoing.

them, and to give a fuller knowledge of the cultures

The achievements attained through these activi-

and the life of the people in tropics.

ties have been published in journals inside and out-

Physicians and professionally qualified persons

side Japan as well as in the "Tropical Medicine", anworking in health and related fields may apply for ad-

official journal of the institute.

mission to the course. Fifteen candidates are accepted to the course in a year. The course consists of
１３weeks （June-August） of instruction and examination. Teaching is undertaken by the full-time staff
and guest lecturers.
The course provids lectures and laboratory andfield practices in virology, bacteriology, protozoology, parasitology, medical entomology, environmental
physiology, biochemistry, pathology, internal medicine, and geography and culture in tropics. Candidates who completed successfully the course areawarded the Diploma in Tropical Medicine.

Adomission ceremony in１
９
９
９

―９―

Training Course in Researchof Tropical Medicine

Public Lecturesat the InstituteArrangements for conducting the course in this In-

The Institute of Tropical Medicine has public lec-

stitute are administered by Japan International Coopera-

tures for citizens annually. The lectures address issues

tion Agency, commissioned by the Government of Japan

pertaining to travellers on tropical diseases. It is intended

to execute Technical Cooperation Programs from １９８３．

to prepare the public to maintain good health abroad. The

This course is conducted by the Government of Ja-

other aim is to open our intellect and knowledge to thepan as a part of its Technical Cooperation Programs for

public.

developing countries with a view of contributing to upgrading their standards in tropical medicine and to pro-

Publications

moting friendly relations to the countries.
The purpose of this course is to help the control ofendemic and epidemic diseases infesting the Torrid Zone.

Tropical Medicine

Through the guidance of the staff of the Institute, the par-This is the quarterly journal that publishes the origi-

ticipants will enrich their fundamental knowledge andnal articles from this institute. The journal was first pub-practical techniques for various medical problems in thelished in March，
１９５９as Endemic Diseases Bulletin oftropics.

Nagasaki University . In１９６７，the name was changed toTropical Medicine .

Individual participants will study on a selected subject in the responsible department for a year. Capacity ofeach department is normally limited to one person.

Institute of Tropical Medicine,
Nagasaki University
The first English guide for foreign visitors wasprinted in １９７１．The revised adition is published every
year.

Adomission ceremony in１
９
９
９

―１
０―

By the letter from Dr. San Tae Han, fromer Direc-

Department of Virology

tor of WHO Regional Office for the Western Pacific（WPRO) dated２
３November１９９３，the Department

This Department has been conducting basic andof Virology, Institute of Tropical Medicine, Nagasaki

applied research on mosquito-borne flaviviral dis-

University was designated as WHO Collaboratingeases such as Japanese encephalitis（JE）, dengue fe-

Centre for Reference and Research on Tropical Vi-ver （DF）/dengue hemorrhagic fever （DHF）, asral Diseases. On ９ August１
９９４，Inauguration Cere-

well as hepatitis C.

mony was held at Pompe Hall by the presence of Dr.

Outbreak investigations on mosquito-borne flaviviral

Han and approximately １２０ guests, which was fol-diseases

lowed by the WHO Workshop Epidemiology andRequested by Japan International Cooperation

Control on DF/DHF and JE . The Department has

Agency（JICA）, outbreak investigation was con-received WHO fellows from Vietnam. Fiji, and theducted on the acute encephalitis which was caused byPhilippines, dispatching WHO short-term consult-Nipah virus in Malaysia from September １
９９８ toants on the subjects relevant to its terms of refer-

March １９９９．Another investigation was carried out,

ence. Dr.Kouich Morita was appointed as the Re-

requested by the Ministry of Health and Welfare, on

gional Advisor on Infectious Diseases, WHO-WPRO,

the West Nile virus encephalitis occurred in New

from１
６May１９９５to１５May１９９８．According to theYork during the summer in１９９９．

letter from Dr. Shigeru Omi, present Director of
WHO-WPRO, the designation of Virology Depart-

Analysis on the structure and function of JE and dengue viral genome

ment as the WHO Collaborating Centre was extended
to２３September２００３．

Nucleotide sequence analysis and molecular expression of JE and dengue viral genome have been
carried out together with long PCR techniques in order to study molecular epidemiology, epitope analysis, biological activity of nonstructural proteins, andvirulent viral gene responsible for viral pathogenesis.
Development of rapid diagnosis on flaviviral diseases
Viral genome detection and identification by
PCR, preparation of diagnostic antigen
from infected cell culture fluid, and simplification of
IgM antibody detection have been carried out.
Activities as a WHO Collaborating Centre

Akira Igarashi
Professor
Assistant professor Kouichi Morita
Research Associate Futoshi Hasebe
Postdoctoral Fellow（COE） Maria del Carmen Parquet
Senior Research Assistant Toshiko Ueno
Senior Research Assistant Mutsumi Nagata
Kazumi Jodai
Technician
Postgraduate student Afjal Hossain Khan
Postgraduate student Shu Ko-ha
Postgraduate student Edward Gitau Mathenge
Paresh Sumatilal Shah
Rompaku Fellow

Laboratory of recombinant DNA experiments（P２‐level）

WHO Workshop―１１―Department of Bacteriology
Our major research interest is to elucidate the
etiologic agents isolated from pathogenic bacteria
related to the worldwide emerging and re-emerging
diseases.
Studies on the cellular and molecular mechanisms of diarrhea induced by bacterial enterotoxins :Aeromonas sobria hemolysin is important in thepathogenesis of diarrhea caused by this enteropathogenic bacterium. By immunoprecipitation analysis
using hemolysin and anti-hemolysin antibody, a ６
６kDa protein（p６６）was identified as a receptor for A.
０７cells. The p６
６wassobria hemolysin on Intestine４
found to be a glycosylphosphatidylinositol-anchoredglycoprotein expressed on the surface of Intestine
４０７cells.（Ref. Microb. Pathog.（１
９
９
９）２
７：２
１
５）
Focusing on the molecular mechanisms of the diarrhea induced by heat-stable enterotoxins（STd）ofenteropathogenic bacteria, we are also studying １）
interaction of Escherichia coli heat-stable enterotoxin with its receptor and ２）
activation of guanylate
cyclase（GC-C）by STa.
（Ref. Eur. J. Biochem．
（１
９
９
９）２
６
３：３
３
８）
Studies on the pathogenesis of Helicobacter pylori :To investigate a potential mechanism of how H.
pylori establishes infection, we investigates the hostparasite relationships of H. pylori , focusing on vacuolating cytotoxin A（VacA）and Cag pathogenicity island （Cag PAI）.
１）VacA exposed to alkaline or acid conditions,with subsequent neutralization, exhibits enhanced
vacuolating activity ; the acid or alkali-activated
VacA appears to bind a cell surface receptor proteinof 〜２
５０kDa. N-terminal and internal amino acid sequence is consistent with the hypothesis that p２
５０is
RPTPβ. Phorbolmyristate （PMA, TPA） induces
differentiation of the human leukemic cell line HL-６０
into cells with macrophage-like characteristics andenhances the susceptibility of HL-６０cells to VacA.
PMA induced expression of RPTPβmRNA and protein as determined by RT-PCR and indirect immunofluorescence studies. Vitamin D３ and IFN-γ,
which stimulate defferentiation of HL-６０cells into a
monocyte-like cells, also induced VacA sensitivity

and expression of RPTPβ mRNA, whereas １．
２％
DMSO and retinoic acid, which stimulated the maturation of HL-６
０ into granulocyte-like cells did not.
RPTPβ anti-sense oligonucleotide inhibited induction of VacA sensitivity and expression of RPTPβ.
Double immunostaining studies also indicated that
newly expressed RPTPβ colocalized with VacA in
PMA-treated HL-６０cells. BKN-２
１cells transfectedwith the RPTPβ cDNA acquired VacA sensitivity.
All data are consistent with the conclusion that acquisition of VacA sensitivity by PMA-treated HL-６０cells results from induction of RPTPβ, a protein thatfunction as the VacA receptor. (Ref. J. Biol. Chem.
（１
９
９
９）
２
７
４：３
６
６
９
３，
J. Biol.Chem.
（２
０
０
０）
２
７
５
：
１
５
２
０
０）
２）Human β-defensin-２（hBD-２）is an antimicrobial peptide which belongs to one of the most important host defence system against bacterial infection in several epithelial tissues. We studied the effect of H. pylori on the expression of hBD-２ mRNAin MKE４
５ gastric mucosal cells. H. pylori , but notculture filtrate, increased hBD-２mRNAlevel inMKN４５cells, whereas thus inductive effect of H. py０
７ cells werelori was not detected when Intestine４
incubated with H. pylori . Among the tested strainsof H. pylori , which lacks Cag PAI, did not induce
hBD-２ mRNA in MKN４５ cells. These results suggested that cag PAI of H. pylori is important for inductive expression of hBD-２ mRNA in MKN４５cells.
Exposure of MKN４５ cells to Salmonella typhimurium, S. enteritidis , S. typhi , and S. dublin ,５，resulted in remarkbut not Escherichia coli ML３able induction of hBD-２ mRNA.
（Ref. Biochem. Biophys. Res. Commun.
（１
９
９
９）
２
８
３
：
７
７
０，
Infect.Immun.
（２
０
０
０）
６
８
：
１
８
０
６）
Studies on the development of cholera vaccine.
The overexpression of fimbriae of Vibrio cholerae O１ is uuder study for use in cholera vaccine
trial.（Ref. Microbiol. Immunol.
（２
０
０
０）４
４
：
４
３
９）

Cytotoxicity of VacA toxin through its binding toreceptor-protein tyrosine phosphataseβ―１２―Professor
Assistant professor
Research Associate
Research Associate
Technologist
Technician
Postgraduate Student
Research Associate

Toshiya Hirayama
Yoshio Ichinose
Masahiko Ehara
Akihiro Wada
Mamoru Iwami
Kayo Honda
Takahiro Kimura
Akitoyo Ichinose

Laboratory

Study of trypanosomes

Department of Protozoology

１）Functions and expression mechanism of transsialidase.

Our main purpose is to clarify infection mecha-

２）Adaptation mechanisms of Trypanosoma species

nisms of intracellular protozoan parasites.

to environments.
３）Modification of infected host-cells by Trypano-soma cruzi .

Study of malaria parasites

４）Simple diagnostic methods for Chagas' disease in１）Specific immune reaction in malaria.
２）Surviving strategies of Plamodium falciparum inmammalian hosts.
３）Epidemiology of human malaria.

endemic fields.
Other studies１）Molecular epidemiology of pathogenic strains ofEntamoeba histolytica in the Philippines.
２）Epidemiology of cryptosporidiosis

Laboratory for culture

Plasmodia sporozoites from ruptured oocysts in
Anopheline mosquito

Professor

Hiroji Kanbara

Assistant Professor

Haruki Uemura

Research Associate

Tetsuo Yanagi

Research Associate

Shusuke Nakazawa

Technician

Miki Kinoshita

Technician

Kurenai Matsuo

Postgraduate Student

Mie Kato

Postgraduate Student

Katsunori Shinohara

Postgraduate Student

Ton That Ai Long

Postgraduate Student

Chaturong Putaporntip

Postgraduate Student

Mohammed Nasir Shuaibu

Postgraduate Student

Maria Cecilia Huaman

Postgraduate Student

Toshio Miyazaki

Amastigotes（left）and trypomastigotes（right）of
Trypanosoma cruzi―１３―

Department of Parasitology
The research activities are concentrated on filariasis and schistosomiasis which constitute the major public health problems in the tropics.
Filariasis
Brugia malayi （Cheju strain, periodic form）, B.
pahangi and the vector mosquito, Aedes aegypti(Liverpool strain）have been maintained in the laboratory for many years. Highlights of recent studies
are as follows.
１）
Mode of action of diethylcarbamazine（DEC）:
Recently we reported that DEC is a competitive
inhibitor of acetylcholinesterase of filarial worms,
DEC inhibits cell proliferation, disrupts the microtubules of LLC-MK２ cells, inhibits polymerizationof microtubules and disrupts the pre-formed microtubles in vitro.
２）Development of a simple and sensitive method for
determination of serum concentration of ivermectin（IVM）and DEC :IVM and DEC modified partially in their chemical structure successfully produced the antibody
against drugs. Therefore the serum concentration（５ ng/ml）of IVM and DEC can be determined by
EIA.
３）Combination of DEC and IVM for the treatmentof filariasis :Several lines of evidence indicate that DEC andIVM interact additively or synergistically against
microfilariae and adult worms of B. pahangi in vivo
and in vitro.
４）Screening of antifilarial drugs from medical
plants :Vernonia amygdalina from Africa, Neurolaena
lobata from Guatemala and Cardiospermun halicacabum from Thailand were effective in vitro on B.
pahangi adult worms and microfilariae.
５）Epidemiology and control of bancroftian filariasis :A research project was carried in Kwale, Kenya,in cooperation with Kenya Medical Research Insti-tute（KEMRI）during the period of１
９９０and１
９９
６．
Transmission potential and morbidity were studied.
Mass-chemotherapy with combination of DEC andNaHCO３ was evalualed.
Schistosimoasis
Schistosoma mansoni （Puerto Rican strain and
Kenyan strain）, S. haematobium （Kenyan strain）andsome strain of vector snails have been maintained in
the laboratory. Highlights of recent studies are as
follows.
１）Swimming behavior of miracidia :cAMP is involved in the control of ciliary beating and chemotaxis of miracidia.
２）Mechanisms of penetration of cercariae into skin :The studies suggest the involvement of protein
kinase C in proteolytic enzyme release from cercariae.
３）Epidemiology and control of S. haematobium infection :Since １９８１，the research project on Schistosomiasis haematobia was carried out in Kwale, Kenya,in cooperation with KEMRI for１５years. The highlights of our studies are human water contact study,
cercarial concentration in natural water, ecology of
Bulinus globosus , usefulness of urinary reagent
strips, new immunodiagnostic test（urine ELISA,
modified COPT）, effect of piped water supply, KAP
study（knowledge, attitute and practices）,health education, morbidity studies using ultrasound and environmental modification for snail control.
Recently high prevalence of bladder cancer and liver
fibrosis is reported in the community.

Ultrasonography of the bladder of the patient infectedwith S. haematobium―１
４―Professor
Assistant Professor
Research Associate
Technologist
Technician
Postgraduate Student
Postgraduate Student
Postgraduate Student
Postgraduate Student
Postgraduate Student

Yoshiki Aoki
Yasunori Fujimaki
Kanji Watanabe
Mitsumasa Miura
Nami Fujii
Tomoharu Ohki
Hidehiko Yamauchi
Hiroshi Matsuyama
Gunawardena Nipul Kithsiri
Teruyo Kusaba

Medical plant used by traditional healer in Kenya

the other hand, the athletes who trained for a longtime are tolerant to heat, as their sweat response issuppressed. The roles of central and peripheral
mechanisms are being investigated.

Department ofThermal Adaptation
Study on the adaptation mechanisms to the hottropical environment in human beings and animals
and its application of tropical medicine are the aims
in the Department, which was newly established in
１９９４．In collaboration with the Department of Environmental Physiology, the following researches areon going.
Study on long-term heat acclimatization in tropical inhabitantsCore temperature, skin temperatures, local
sweat rates, and metabolic rate during heat load or
exercise and Ach sensitivity of the sweat gland arecompared between Japanese and tropical inhabitants（Thai and Africans）. It has been revealed that in a
hot environment, tropical inhabitants can efficiently
regulate their body temperature with less amount ofsweat compared to Japanese, because of their excellent dry heat loss ability（conduction, convection andradiation） and suppression of ineffective sweating
which drips off the skin. Suppressed sweating in
tropical residents is attributed to adaptation in both
central（upward shift of threshold core temperaturefor sweating and suppression of the gain）and peripheral（suppression of Ach sensitivity of the sweat
gland）sudomotor mechanisms. This stucy is carriedout in collaboration with the Department of Physiology, Chiang Mai University, Thailand.
Study on cross-adaptation of physical training to heat
After a short-term physical training, heat tolerance is enhanced by the activated sweat response. On

Study on cellular and molecular heat-tolerance in temperature sensitive mutant cells
The temperature-sensitive mutant from mousebreast cancer cells, tsFT１０１，is multinuclei when
cultured at ３
９℃. The induction of thermotolerance
and also the induction of heat shock proteins due topre-heating at４５℃ for３
０min are very little in tsFT
１０１ cells cultured at ３
９℃.The mechanisms of thermotolerance in cellular and molecular level are underinvestigation by using this cell line.
Study on cold adaptation in the weak heattolerant animal, pika（Ochotona）
The pika（Ochotona）living in the cold areas or
high mountains attracts attentions as an animal modelof cold adaptation as well as highaltitude adaptation.
Th pika is intolerant to heat for lack of efficientheat loss mechanisms, and does not hibernate in winter. To clarify the cold adaptation mechanisms in pikas, physiological studies on atonomic and behavioral
thermoreguration, anatomical studies and the fieldstudies in their natural habitats in China and Mongolia are on going.
Comparison of thermal adaptability among tropical, subtropical and temperate subjects
We compare the natural environmental adaptability through out the effect of environmental temperature and sunlight on functional, morphological,
and organic changes in tropical（Thai）, subtropical（Nepal） and temperate（Japanese）subjects.

Experiment of thermal sweating in the environmental
chamber―１５―Professor（concurrent）
Research Associate

Eiko kaneda

Laboratory of cell culture

For the expression of gp９１phox in these cells, weDepartement of Biochemistrypreviously suggested a common transcriptional activator which requires the position-５３ based on an

Our research interest is focused on the molecu-

analysis of a novel patient with chronic granuloma-

lar events ocurring in inflammatory cells for the de-tous disease. HAF-１ and PU．
１were indentified as

fense against invading microbes.

Reactive oxygencandidates for this activator. We, therefore, exam-

species are essential for killing most of bacteria,

ined ６０ fragments with onebase substitutions neigh-fungi, and parasites. We are therefore investigating

boring to-５３，and found two sequences ; one has amechanisms for the expression and activation of

mutation at-５６and impairs the binding of HAF-１，

superoxide-degenerating NADPH oxidase system.

and the other mutation at-５０and impairs the binding

GATA-３ as the Eosinophil-specific Repressor for theof PU．
１The-５０mutant promoter but not-５６ mutant

Expression of gp９１phox, an Electrontransferring Compo-

one exhibited decreased reporter activity, indicating

nent in Phagocyte NADPH Oxidase System

PU．
１，but not HAF-１，to be an essential activator

In a systematic search for cis-elements regulat-

for the expression of the gene in those cells （Fig．
１）．

ing gp９
１phox exression in eosinophil lineage, we identi-

A discovery of a similar patient with a point mutation

fied an inhibitory element containing a GATA con-at-５２ which abolished the binding of PU． １ con-sensus site at the proximal promoter and GATA-３

firmed our conclusion.

as the specific protein binding to that site. Two-base

Our future aim is to apply these findings to in-pair substitution at the consensus site abolished in-innovation of techniques to control tropical diseases

hibition of the promoter activity in eosinophil-and allergy.

committed HL６
０-C１
５ cells, indicating that the
GATA-３ binding to the site is a repressor in theProfessor

Michio Nakamura

cells. Because eosinophil is the only cell expressing

Assistant Professor

Atsushi Kumatori

GATA-３ among human phagocytes and B lympho-

Research Associate

Shoichi Suzuki

cytes, GATA-３ is an eosinophil lineage-specific re-Technologist

Toshiyuki Moriuchi

pressor of gp９
１phox gene.

Postgraduate Student

Hao Li Jun

Postgraduate Student

MD. Rafiqul Islam

Postgraduate Student

Maki Takata

Postgraduate Student

K. A. Deepa

Visiting Researcher

Yang TanPU．
１but not HAF-１ is a Common Activator for
the Expression of gp９
１phox in Neutrophils, Monocytes and
B Lymphocytes-56G m-50G m
-53T m
Wild
pXP2
0

100

200

300

400

500

Relative LUC Activity

Fig．
１．Reporter gene expression driven by various mutant gp９
１phox promoters―１６―Facilities consist of histology, immunopathology

Department of Pathology

and molecular pathology laboratories, and electron
microscopy.

Research projects are fundamentally geopa-

Present research projects are as follows :thological and histopathological investigation of

１．Pathology of tropical diseases including infec-

tropical diseases in collaboration with other over-tions, neoplasms, and other diseases.

seas or domestic institutions.

２．Liver diseases in the tropics such as viral hepa-

The pathology department is responsible fortitis, cirrhosis of the liver, hepatocellular carcinoma,

postmortem examinations and histological diagnostic

and other endemic diseases.

pathology of surgical speciments including special

３．Geopathology, histopathology and molecular pa-

research work on liver biopsy at the Nagasaki Uni-

thology of Kaposi’s sarcoma

versity Hospital.

４．Geopathology of tumors and viral oncogenes in

In addition to the routine services mentioned

East Africa.

above, the department is also responsible for lec-

５．Geopathology of tumors in South-east Asia.

tures and practice for the undergraduate students at

６．Interdisciplinary study on environmental pathol-Nagasaki University School of Medicine on pathol-ogy in the tropics :ogy of the hematopoietic system, liver, gallbaldder,ecology and disease manifestations.

environmental factors, humanbiliary ducts and pancreas.
The postgraduate course of ordinary pathology,

Professor

Hideyo Itakura

tropical pathology and liver pathology for graduate

Associate Professor

Kan Toriyama

students of medicine is a four-year program leading

Research Associate

Masachika Senba

to Ph. D.

Research Sssociate

Masachika Iseki

Technician

Akemi Arao

The five-year professional pathologist programis offered, leading to the qualified pathologist of theJapanese Society of Pathology.

Laboratory of microtome

Histology slide conference―１７―．Rapid diagnonsis by molecular biological techniqueDepartment of Internal Medicine
Our main studies are described in the followings.
．Comparative study on respiratory infections between
Thailand and Japan
Our department has promoted collaboration
study on respiratory infections with Chaing Mai University in Thailand. Since then the pathogens of respiratory infections has been recognized. As a result
the antimicrobial treatment has been appropriately
modified.

．The laboratory and clinical studies on bacterial respiratory infections

．Studies on anti-inflammatory mediator therapy inchronic respiratory infections
Our studies have revealed that the neutrophil
activator, IL-８ and neutrophil elastase, play a very
important role in chronic respiratory infections. The
studies for anti-mediator therapies are undergoing.

．Basic and clinical studies on nosocomial infections by（PCR）
Using PCR（Polymerase Chain Reaction）technique, it is possible to detect the pathogens rapidly
even if a few number of pathogens are present inclinical specimens. We are using the PCR for the diagnosis of mycobacterial infections and obtaining
satisfactory results.

．Collaboration study in Uganda on the treatment of

AIDS patients with infections（tuberculosis, cryptococcal meningitis and e.t.c）
Since１９９０we have investigated the condition of
AIDS in Uganda to save patients complicated with
infections. Caollaboration project has been with the
School of Medicine, Makerere University, Uganda.

．Basic and clinical studies on influenza
．Chinical study on acute respiratory infections andbacterial meningitis among the children in Bangladesh．Study on Bacterial adherence of respiratory infection.

Staphylococcus aureus
Staphylococcal infection is one of the serious
problems of nosocomial infections. We established amethod for the management of nosocomial infections.
Although we could reduce the occurrence of MRSA
infections, there are various problems concerning
this organisms particularly its pathogenecity.

．Analysis of the normal nasal and oropharyngeal

flora in children and pediatric patients with upper respiratory infections

Laboratory

Professor
Associate Professor
Research Associate
Research Associate
Research Fellow
Postgraduate Student
Postgraduate Student
Postgraduate Student
Postgraduate Student
Postgraduate Student

Tsuyoshi Nagatake
Kazunori Oishi
Hideaki Amano
Kiwao Watanabe
Kamruddin Ahmed
Kazushi Motomura
Tomoo Ukon
Yuko Yamaguchi
Mariko Saito
Junko Goto

Laboratory―１
８―

HTLV-I-infected cells have been shown to have

Department of Preventive
Medicine and AIDS Researchhigh levels of active NF-κB and Tax has been demonstrated to active some cellular genes by causing an
increase in HF-κB levels. However, in the ATL sam-This department was newly added to the insti-

ples, viral mRAN are not detected. These results in-tute in １
９７
８ as a research division open to visiting

dicate that the celluar genes are constitutively over-professors from other universities and institutes. It

expressed in the absence of Tax in vivo. These find-is run by concurrent research staff for the present.

ings imply that there is another mechanism independ-

We have planned and started from January of１
９９７a

ent of Tax underlying the NF-κB activation in fresh

series of fundamental research to answer the ques-

ATL cells. The mechanism of Tax independent NF-

tion how and what mechanisms retroviral infection

κB activation is under investigation by using TL-

may lead to several diseases including acquired im-

Oml cells.

munodeficiency syndrome（AIDS）and adult T-cellleukemia （ATL）.

Study on mechanism of apotosis induction in HIV or
HTVL-I infection
Generally, the length of time between HIV infec-

Study on the mechanism of transactivation of severaltion and development of AIDS is considered to becellular genes by HTLV-I-Tax and HIV-Tat
HTLV-I and HIV are known to be causative

１０ years on average. HIV infection is accompanied

agents for ATL and AIDS, respectively. HTLV-I-

by the progressive loss of CD４ Tcells. Apoptosis, a

Tax and HIV-Tat are nuclear proteins which tran-

form of programmed cell death, has been implicatedscriptionally trans activate not only their own en-

in pathogenicity related to infection with HIV.

hancers in the long terminal repeat but also a number

HTLV-I Tax also leads to apoptotic cell death.

of cellular genes. We have previously demonstrated

Apoptotic pathway and its mechanism which accountthe capacity of the Tax of HTLV-I to modulate thefor the pathophysiology in HIV or HTLV-I infected

expression of various cellular genes : the cytokine

individuals are under investigation by using Tcells

genes for IL-１α, IL-６，IL-８，IL-１０，and the celltransfected to constitutively express Tat or Tax.

adhesion molecule gene for ICAM-１．We are now intending to study the mechanisms of transactivation ofVisiting Professor

Naoki Yamamoto

cellular genes including IL-８，IL-１
０，ICAM-１，and

Visiting Associate Professor

Takao Masuda

iNOS by Tax or Tat.

Research Associate

Naoki Mori

Technician

Masako Sasaki

Study on the mechanism of Tax independent NF-κB activation

Laboratory for biochemical research

Tissue culture room

―１
９―

acters among gegraphical strains. Feeding behavior

Department ofMedical Entomology

and population dynamics of these mosquitoes also arestudied in the laboratory and fields.
３．Vector control technique related studies
Basic study of copepods as hopeful biological

Main interest in the department is analysis ofenvironmental factors that affect the trasmission of
insect-borne diseases, and pursuing better vector

control agent against vector mosquito larvae. Biochemical approach to insecticide registance in vector
mosquitoes.

control strategy that is harmonious to the environment.

Professor

Masahiro Takagi

Assistant Professor

Yoshio Tsuda

Research Associate

Nobuko Tuno

laria has been conducted at several fields in South-

Technician

Emiko Urakawa

east Asian countries. Spatial and temporal changes

Postgraduate Student

Yukiko Higain larval habitats, vegetation, and the impact of hu-

Postgraduate Student

Ronald Enrique Marales

man activity on the environment are evaluated in re-

Postgraduate Student

Hamady Dieng

lation to abundance of vectors, host preference, andPostgraduate Student

Tomomitsu Satoother population parameters. The GIS/RS is intro-

Postgraduate Student

Maiko Hasegawa

duced as a new tool to analyze the environment. Geo-

Roupaku Student

Wannapa Suwonkerd

１．Physiology and ecology of malaria vectors
A long-term monitoring on main vectors of ma-graphical strains of several Anopheles are comparatively studied by morphological, physiological, andgenetic approach.
２．Ecology and control of dengue vectors
Epidemiological studies have been conducted atseveral fields in Southeast Asian countries. Monitoring by oviposition traps and mark-release-recaptureexperiment are performed to establish more realistic
larval indices. In the laboratory Aedes aegypti andAe. albopictus are studied on their ecological char-

Stereomicroscopic observation of mosquitoes

Ramp traps for collecting mosquitoes―２０―Department ofSocial Environment
This Department, started five years ago, covers
interdisciplinary field of Environmental and Social
Medicine related to health services and social welfare problems in developing countries.
Interdisciplinary approach includes studies in
Social Sciences and Humanities. Further, methods
and actual means of international cooperation toward
developing countries has been sought to utilize in order to promote inter-departmental linkage for information accumulated with specific analysis and professional response to the public particularly in thefield infectious diseases control.
Under this context, basic and applied research
have been carried out on the following prospective
themes from the view point of environmental and social aspects for the purpose of promoting assistanceto minimize health problems in tropical areas :１．Study and analysis on social (incl. life style, political and economical) background which regulates
frequency / areas / combination of diseases of thepresence of epidemics.
２．Study on the effect of individual and social environment toward endemic and epidemics.
３．Standardization of human security measures in
the field of health services from the view points ofnutrition and ３Es ie.economy, environment &education.
４．Study on control of communicable diseases
among the regional and international health careprograms.
５．Study on quantitative and qualitative values of
medical and health services under the ODA ie. Official Development Assistance programs by Japanese government.
６．Feasibility study on comprehensive information

Dept. of Social Environmental Medicine often serves
as a secretariat for Int’l Symposia.

filing and network system for the sake of promoting health and social welfare services in developing countries, by epidemics, by regions, by administrative measures.
７．Reformation and coordination of health manpower traning program / system to meet the needof tropical area.
８．Comparative studies on the control of imfectious
diseases in tropical Asia.
Specific themes of joint cooperative researchand research seminar designated to the Department
in FY２０００ under the scheme of Research Institutefor Nationally Joint-Use are listed below. The
Technical and Management Committee recommended
that interdisciplinary research collaboration should
be realized from the viewpoint of middle and longterm range and managed not only by single department but by the whole Institute as one of the important mandates.
Joint Research projects’s themes :a ）Linkage and balance between mass-poverty / environment / culture, and control measures fortropical medicine.
b ）Historical study on tropical medicine.
c ）Study on HIV / AIDS epidemic in Eastern Africa.
Research Seminar topic :Study on comparative element and balance between development / environment / culture for control and prevention of tropical diseases.
Professor
Research Associate
Research Associate
Research Assistant
Postgraduate Student

Tsutomu Mizota
Taro Yamamoto（on mission）
Susumu Tanimura
Eiko Tara
Chizuko Suzuki

Active chairmanship role creates interdisciplinary cooperative fruits.

―２
１―

Department ofEnvironmental Physiology
Since １９
７
７，this Department has been studying
the interactions between tropical environment andthe human beings and animals from the view point of
the environmental physiology and aiming to apply the
results to tropical medicine. In １９９６，the Magnetic
Resonance Imaging（MRI）for animal experiment was
induced in this Institute. The two environmental
chambers in which air temperature and relative humidity are controlled belong to the Department.
Study of effective protect method from ultraviolet rays
Ultraviolet rays（UV）contained by Sun lightcause various damage in the living body, and UV is alarge quantity and strong power in the Tropical Zone
and the mountains in particularly. Therefore, thecreatures on earth acquired various protect methods
for ultraviolet rays harmful to body in a process ofevolution.
We have been promoting the study of protect
methods from ultraviolet rays in Yellow mouse inhabiting desert, Pika inhabiting the mountains of
３２００m above see level and other several kinds of wild
mammals.
In this study, we examine countermeasures forultraviolet rays in the Tropical Zone and ozone holein the environmental disruption. For the purpose, we
study effective protect mechanisms for ultraviolet
rays from wild mammalian animals, which inhabit desert or the mountains for many generations understrong UV conditions.

The influence of change in biophylaxis functions induced by ultraviolet radiation to infection of tropical disease
Skin cancer occurs by strong ultraviolet radiation for a long term. The exposure to more little
mount of ulrtaviolet rays may be influence biophylaxis function to infection. Ultraviolet rays in the
Tropical Zone are strong, and many persons sufferfrom infectious disease. There are approximately
２５０ million patients with schistosomiasis in Tropical Zone.
In our experiment on infection of Schistosoma
mansoni cercariae to mouse, ultraviolet radiationgroup by ultraviolet lamp（UV-B）was statistical differencei the number of Schistosoma mansoni cercariae invaded into skin and recovery the adult warms
compared with non-irradiation group. We experiment
the influence of ultraviolet rays in Sun light on it
now.
This study elucidates influence of ultraviolet
rays for infectious disease and contributes to the infectious disease control, in particular, it in the
Tropical Zone with strong ultraviolet rays.
Study on the mechanisms of heat and cold tolerance inhibernators
Hibernators possess a characteristic ability totolerate cold. To clarify the mechanisms of cold tolerance, the neural, endocrine and cardiovascular systems and the induction of cold shock protein are being compared between hibernators and nonhibernators （pika, rat and rabbit）.
Study on the coordination of extensor and flexor muscles
in ballistic action by surface electrode myogram and encephalogram analyzer
During ballistic finger extension, flexor muscles
coordinate with extensor muscles. The significanceof coordination of flexor and extensor muscles is under investigation in the athletes who trained ballistic
actions.
Associate Professor
Reserch Associate
Technician
Technician

Nobu Ohwatari
Jeong-Beom Lee
Junko Kawashima
Junko Hayashima

Yellow mouse inhabiting desert

Pika inhabiting the mountains of３２００m

Environmental controlled chamber and the data acquisition and analysis system―２２―

Department of Internal Medicine

Animal Research Center（University Hospital）
In December１９７４，Prof. Keizo Matsumoto（imcumbent Emeritus Prof.）arrived as a professor of
the Department of Internal Medicine, from when theWard of Internal Medicine in the University Hospital
started its real activities. Since April １
９９４，Dr.
Tsuyoshi Nagatake has succeeded as a next professor after the retirement of Prof. Keizo Matsumoto.
The department is now one of the leading in the fieldof infectious diseases in the respiratory tract and infectious diseases in the tropical area. Besides lectures and training for medical students we make research on respiratory infections using quantitative
culture method with inflammatory cytology of the
sputum as well as bronchial secretes taken throughbronchofiberscope. Antibiotic concentration in clinical specimens and susceptibility of causative organisms to various drugs are also measured to evaluate
efficacy of chemotherapy. Clinical training for a doctor as a trainee is done to make a high quality physician, as not only a specialist for infectious field but ageneral physician for whole internal medicine.
Professor and Chief
Associate Professor
Assistant Professor
Research Associate
Research Associate
Research Associate
Research Fellow
Research Fellow
Research Fellow
Research Fellow
InternInternTsuyoshi Nagatake
Kazunori Oishi
Hiroshi Watanabe
Norichika Aso
Hiroyuki Yoshimine
Shouzaburo Onizuka
Yoshiko Tsuchihashi
Hiroaki Mitsushima
Reiki Kuroki
Shinichi Kurita
Naoki Tashiro
Mayumi Terada

The center makes it the principal aim to ensure thesafety of animal experiments which deal with the pathogenic microorganisms and to build up the successful generation of experimental animals, microorganisms andparasites. The building consists of７ breeding rooms ofexperimental animals，
２laboratories, one breeding roomof snails, one insectarium, two P３-level biohazard laboratory and breeding room. The air pressure is kept to be always negative to avoid outflow from inside even at entrance to building. Since the building has the most thorough ventilation through HEPA filter, the microorganisms could not leak out to outside of the building. The
water used is given chlorination and drained off. The
breeding of animals and experiments are done according
to the guideline for animal experiment edited by Nagasaki University. The laboratory animals bred in thecenter are mice, rats, hamsters, rabbits, gerbils, snails andmosquitoes. The center is largely used for the original
research by Department of Virology, Bacteriology, Protozoology, Parasitology, Biochemistry, Environmental
Physiology and Internal Medicine.
Professor and Director
Research AssociateMichio Nakamura
Akiyoshi Utsunomiya

Mongolian gerbil （ Meriones unguicalatus ）Clinical conference―２
３―

Infomation and ReferenceCenter of Tropical Medicine

Central Laboratory

The Information and Reference Center was es-

Many up-to-date equipements listed below aretablished in April １
９９７．The center provides neces-

ready in use in the laboratory. Electron microsco-

sary information and references related to tropicaldiseases to all the staff,

graduate students,

re-searchers and trainees, as well as members of thepies（TEM and SEM of Jeol）, a Ultramicrotome(Reichert）, a flow cytometer（FACScan）, a cellpublic who visit the Institute. Currently available atsorter（FACSsatar plus）,scanning electron micros-

the center are books, maps, periodicals, pamphlets,

copies（Bio rad MRC６００and Ueiss LSM）, microma-films, videocassette tapes, photo slides, photographs,

nipulation systems（Nikon-Narishige and Zeiss-charts, exhibition panels, animal specimens as well as
pathological tissues specimens. These materials are
now being digitized and are provided to the public

Shimazu）, Bioimage analizers（Hamamatsu Pheotonics, Bio rad GS-２５０and Pharmacia Image Master）,athrough cyberspace. The center also gathers scien-

peptide synthesizer（Millipore ６００E）, a peptide se-tific, social economic and cultural information re-quencer（ Shimazu

lated to tropical diseases.

（Perkin-Elmer ３
７３A-７０ and Pharmacia AFL）
, a

The center carries out eco-epidemiologicalstudies on tropical diseases to project the future oftropical infectious diseases from both local and

PPSQ-１０）,DNA

sequancers

DNA / RNA extraction system（Perkin-Elmer３
４１３０），a real-time surface plasmon detector（Fisons

global perspective. Taxonomy and ecology of vector

IAsys）, two P３ rooms, Super centrifugal machine

insects and mites of infectious diseases are also the（optima L-９０K）. Many kinds of experiments for cellresearch subjects of the center.

biology can be carried out with use of these equipements and others set in individual departments.

Professor

Masaaki Shimada

Associate Professor

Hiroshi Suzuki

Research AssistantToshihumi Oyama

Research AssistantChika Yamaguchi

Technician

Kiyomi Suda

Professor and Chief

Toshiya Hirayama

Research Associate

Akitoyo Ichinose

Postgraduate Student Tomoko Kisu
Postgraduate Student Yoshiki Hamada

Exhibition room

The TEM of electron microscope Laboratory―２４―

Library

Administration

Although there were systematic changes in Na-

Shunichiro Nakamura, Head Official

gasaki University Library, the library in this institute, a branch of Medical Library, has been perform-

General Affairs Unit

ing the same activities as beginning in１９４
９．This li-

Kou Mihara, Chiefbrary possesses approximately ９８４０ professional

Yoji Hashiguchi, Sub-Chiefbooks and ４
６
７ periodicals in the various fields ofNanami Tsuji, Assistant Staff

tropical medicine especially related to infectious dis-

Toshiko Ueno, Research Assistanteases. Most of them are arranged in the library forfree reading by the staff of the Institute and author-

Accounting Unit
Hisao Ii, Chief

ized persons.

Kouzou Aota, Staff
Professor and Chief

Tsutomu Mizota

Kazuki Ikeno, Staff

LibrarianKiyoko Baba

Yumiko Yamada, Assistant Staff
Asuka Matsuo, Assistant Staff
Collaboration Research Unit
Chikayoshi Aoki, Chief
Narumi Sasaki, Staff
Junko Suenaga, Assistant Staff

New arrivals cornerBook shelf corner

Administration office―２
５―

Location map of the Institute ofTropical Medicine on SakamotoCampus of Nagasaki University

Playground;;Gymnasium

Laboratory Animal Center
for Biomedical ResearchRadioisotope
CenterParking

Medical Materials
Center for Atomic
Bomb Casuality

Sub-Gate

Information and ReferenceCenter of Tropical Medicine
In part of the
3rd and 4th Floor()

Main Building of
the Institute ofTropical Medicine

School of Medicine
AtomicDiseaseInstitute

Animal Research Center

ポ
ン
ペ
会
館

Main Gate
MedicalBranchLibrary
AlumniHall

Commemoration
Hall

Back Gate

Pompe
Hall

N

for University Hospital

―２
６―

137

Electoron Microscopes

Ground plan of the Institute136

Central Laboratory

1st Floor
Administration
Library
Central Laboratory（in part）
Department of Pathology
Department of Thermal Adaptation
Department of Molecular
Epidemiology

135

ToiletLibrary

138

EPS
Seminar
room

139

134

140

Meetingroom
Lecture
room

120

117

121

Trainingroom

Trainee'sroom

116 Department

122of Molecular
Epidemiology

Toilet115 Department

123

of Thermal
Adaptationａ Informationｂ Professor's Officeｃ Associate Professor's Officeｄ Laboratory

133

124

114

Insetarinm
Ｃ,Ｄ
125

113

Insetarium
Ａ,Ｂ

Lift

d6
112

126

d4
132

101

131

129

Head
Official's
OfficeDean's
Officed5
111

129 127

Toilet

c

dl
110

a
102

Administration

103

104

Copy
room

Night
duty
room

Department of Pathology

d2
Entrance 105

240

238

Laboratory
（P3）

106

107

242

d

d
244
（P3）

235

a

234

d2

d1

247

d2

d
（P3）

b

232

Lift

c

231

201

221

218

a
217

222

Toilet229

Central Laboratory
230

Department of Parasitology

d3

d1

215 Research

Associate's
Office
224

228

d4

b

216

223

ａ Informationｂ Professor's Officeｃ Associate Professor's（or Lecture's） Officeｄ Laboratory

Department of Protozoology
233

219

c

Department of Lnternal Medicine
Department of Protozoology
Department of Preventive Medicine
and AIDS ResearchCentral Laboratory

d
246

109

220

2nd Floor
Assosiate's
OfficeDepartment of Parasitology

d4
245

108

Microtomesa243

Toilet EPS

Stainingroom

237

Department ofPreventive Medicine and AIDS Research 236 Reserch
241

d3

b

Central
239

b

214

225

213

226

212

CentoralLaboratory

d3
d5
d4

227

211

d3
202

d

203

Department of Internal MedicinedCentral Laboratory

a
204

b
205 206

―２
７―

207

d1
208

d2
209

210

343

344

342

ａ

Department of Medical Entomology

d1

341

d2

ｂ

3rd Floor
Department of Virology
Department of Bacteriology
Department of Social Environment
Department of Medical Entomology
Central Laboratory
Information and ReferenceCenter of Tropical Medicine

340
345 346

d3

Toilet

d

d

347
339

EPS

348

Computerroom

Staff room

338

c
349

337

Exhibition
room（1）

Exhibition
room（2）

Information and ReferenceCenter of Tropical Medicine
336 Audio-

Visual
room
350

335

Computer
room

ａ

Lift

321

320

CentralLaboratory

d5

319

c
318

322

b

Toilet323

317

Research
Associate's
Office
316

324

d4
315

325

d3
301

334

b

333

332

330

CentralLaboratory

302

329

328

d8

Toiletd9

331aDepartment of Virology

ａ Informationｂ Professor's Officeｃ Associate Professor's（or Lecture's） Officeｄ Laboratory

d2

326

314

327

（P2）

d1

303

Department of Bacteriology
Department ofSocial Environment

d1

304

305

d2
306

b
307

d3
308

a
309

d4
310

d5
311

d6
312

414

415

Department of Biochemistryad1

4th Floor
Department of Environmental
Physiology
Department of Biochemistry
Central Laboratory （in part）
Section for Experimental
Small Animals
Information and Reference Centerof Tropical Medicine （in part）

413
416

417
EPS

Toilet

d2

b

419

412

418

a

d3
420

411

d

b

Information
and ReferenceCenter of
TropicalMedicine

410

421

409

d6

d1

d2

Department of Environmental
Physiology
408
Lift

407

401
Research
Associate's
Office
402

404

d4

d5

422
Biotron foranimalsd3
403cａ Informationｂ Professor's Officeｃ Associate Professor's（or Lecturer's） Officeｄ Laboratory

406

Central laboratory
405
Biotron forhuman being

―２
８―

423

424

425

Section for Experimental
small animals

426

d7
313

Ground plan of the Animal Research Center

Information

Snailrearingroom

1st Floor

Insectarium

Storage（2）
Air
showerMachinery

Entrance
Lift
Laboratory forbiohazard

Small
animalrearing
room
（1）

Small
animalrearingroom（2）

Storage（1）Smallanimalrearingroom（3）

Middleanimalrearingroom（1）

2nd Floor
AutoclaveExperimental
infectionroom（1）

Experimental
infectionroom（2）

Lift

Infected animal
rearing room
（1）

3rd Floor

Middle animal
rearing room
（2）

Infected animal
rearing room
（2）

Air
conditioning
machinery

Autoclave
Lift
Immune animal
rearing room―２
９―

Infected animal
rearing room（3）

Telephone Number
Institute of Tropical Medicine, Nagasaki University ………………………………………０
９５（８４９）７８００
Extensions
Dean ………………………………………………………………………………………４７００

８４９‐７８０１

Head of Administrative Office ……………………………………………………………４７０１

８４９‐７８０２

Chief of General Affairs Unit ……………………………………………………………４７０２
General Affairs Unit ………………………………………………………………………４７０３

８４９‐７８０３

Facsimile …………………………………………………………………………………４７０５

８４９‐７８０５

Chief of Accounting Unit …………………………………………………………………４７０６
Accounting Unit …………………………………………………………………………４７０７

８４９‐７８０６

Accounting Unit …………………………………………………………………………４
７０８
Chief of Collaboration Research Unit ……………………………………………………４７０９
Collaboration Research Unit ………………………………………………………………４７１０

８４９‐７８０７

Meeting Room ………………………………………………………………………… ４
７１１
Night Duty Room …………………………………………………………………………４７０４

８４９‐７８０４

Department of Virology
Professor …………………………………………………………………………４７３３

８４９‐７８２７

Associate Professor ………………………………………………………………４７３４

８４９‐７８２８

Information ………………………………………………………………………４７３５

８４９‐７８２９Facsimile …………………………………………………………………………４７３６

８４９‐７８３０

Department of Bacteriology
Professor …………………………………………………………………………４７３７

８４９‐７８３１

Lab．
９ ……………………………………………………………………………４７３８

８４９‐７８３２

Information ………………………………………………………………………４７３９８４９‐７８３３

Department of Protozoology
Professor …………………………………………………………………………４７４１

８４９‐７８３５

Associate Professor ………………………………………………………………４７４２

８４９‐７８３６

Lab．
３ ……………………………………………………………………………４７４３

８４９‐７８３７

Information ………………………………………………………………………４７４４

８４９‐７８３８Department of Parasitology
Professor………………………………………………………………………４７２８

８４９‐７８２２

Associate Professor……………………………………………………………４７２９８４９‐７８２３

Research Associate……………………………………………………………４７３０

８４９‐７８２４……………………………………………………………………４７３１

８４９‐７８２５

Information

Department of Molecular Epidemiology
Professor………………………………………………………………………４７７０

８４９‐７８６０

Department of Thermal Adaptation
Professor （Concurrent）…………………………………………………………４７２４

８４９‐７８１８Department of Biochemistry
Professor …………………………………………………………………………４７５４

８４９‐７８４８

Lab．
１ ……………………………………………………………………………４７５５

８４９‐７８４９Lab．
２ ……………………………………………………………………………４７５６

８４９‐７８５０

Information ………………………………………………………………………４７５７

８４９‐７８５１―３０―

Department of Pathology

Extensions

Professor …………………………………………………………………………４７
１９

８４
９-７８１
８

Associate Professor ………………………………………………………………４７
２０

８４
９-７８１
４

Lab．
２ ……………………………………………………………………………４７
２１

８４
９-７８１
５

Information ………………………………………………………………………４
７
２２

８４
９-７８１
６

Department of Internal Medicine
Professor …………………………………………………………………………４７
４６

８４
９-７８４
０

Associate Professor ………………………………………………………………４７
４７

８４
９-７８４
１

Information ………………………………………………………………………４
７
４８

８４
９-７８４
２

Facsimile …………………………………………………………………………４７
４９

８４
９-７８４
３

Department of Preventive Medicine and AIDS Research
Professor …………………………………………………………………………４７
５０

８４
９-７８４
４

Research Associate ………………………………………………………………４７
５１

８４
９-７８４
５

Information ………………………………………………………………………４
７
５２

８４
９-７８４
６

Department of Medical Entomology
professor …………………………………………………………………………４
７
１６

８４
９-７８１
０

Staff room ………………………………………………………………………４
７
１７

８４
９-７８１
１

Information ………………………………………………………………………４
７
１５

８４
９-７８０
９

Facsimile …………………………………………………………………………４７
１８

８４
９-７８１
２

Department of Social Environment
Professor …………………………………………………………………………４７
７４

８４
９-７８６
４

Information ………………………………………………………………………４
７
７５

８４
９-７８６
５

Information ………………………………………………………………………４
７
７６

８４
９-７８６
６

Facsimile …………………………………………………………………………４７
７７

８４
９-７８６
７

Department of Environmental Pyhsiology
Professor …………………………………………………………………………４７
２４

８４
９-７８１
８

Associate Professor ………………………………………………………………４７
２５

８４
９-７８１
９

Information ………………………………………………………………………４
７
２６

８４
９-７８２
０

Facsimile …………………………………………………………………………４７
２７

８４
９-７８２
１

Animal Research Center
Information ………………………………………………………………………４
７
６２

８４
９-７８５
６

Laboratory ………………………………………………………………………４
７
６３

８４
９-７８５
７

Information and Reference Center of Tropical Medicine
Professor …………………………………………………………………………４７
６０

８４
９-７８５
４

Associate Professor ………………………………………………………………４７
５９

８４
９-７８５
３

Computer Room………………………………………………………………４７
７８

８４
９-７８６
８

Information ………………………………………………………………………４
７
６０

８４
９-７８５
４

Facsimile …………………………………………………………………………４７
７９

８４
９-７８６
９

Central Laboratory
Electron Microscope Room ………………………………………………………４
７
６５

８４
９-７８５
９

Computer Room …………………………………………………………………４７６６
FACS Room………………………………………………………………………４７６７
Section for Experimental Animals ………………………………………………４７６９
Library ……………………………………………………………………………………４７
１４

８４
９-７８０
８

Location map of the Institute of Tropical Medicine,
Nagasaki University in Nagasaki City

INSTITUTE OF TROPICAL MEDICINENAGASAKI UNIVERSITY

Mt. Inasa

Ｎ

Ropeway

Nagasaki Bay

Urakami

fo

rK

ak

for Akasako−
OhashiuraHamaguchi-machi
Urakamieki-mae
Nagasaki−
Ohato

Igakubu-me

Nagasakieki-mae

School of
Dentistry
University
Hospital

School ofMedicine

Urakami−tenshudo-mae

Isibashi
Tsukimachi

Prefectural OfficeInstitute of Tropical
Medicine

City Hall−−
Chuobashi−−
Kokaido-mae
Suwajinja-mae−
Shokakuji-shita

JR Kyushu
Streetcar
Main bus routfor Mogi

for Hotarujaya

How to get the Institute
１．From Nagasaki Station （JR Kyushu）
Get on a bus (Nagasaki Bus, Line ８）at Nagasakieki-mae and get off at Igakubu-mae.
Five minutes walk from the bus stop.
Get on a streetcar at Nagasakieki-mae（for Akasako, Line １or３）, and get off at
Hamaguchi-machi. Ten minutes walk from the streetcar stop.
Ten minutes by taxi from the station.
２．From Urakami Station（JR Kyushu）
Twenty minutes walk.
Five minutes taxi.
３．From Nagasaki Airport
Get on limousine at the airport（for Nagasaki）, and get off at Nagasaki-ekimae, andthen continue to１．
Get on limousine at the airport（for Nagasaki）, and get off at Ohashi, and then twenty
minutes walk from the bus stop or ５ minutes by taxi.
About one hour by taxi from the airport.
Location
１‐
１
２‐４ Sakamoto Nagasaki ８５２‐
８５２３
URL http : //www.tm.nagasaki-u.ac.jp

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熱研案内パンフ（英文）＊ＣＭＹＫASFOU ND E D 1 9 42

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I UNIVIT

SEPTEMBER

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Published on September ８，２０００Edited by the Information and Reference Center of Tropical Medicine, Institute of Tropical Medicine,
Nagasaki University
Printed by Showado Printing Co.,１００７‐２ Nagano machi, Isahaya８
５
４
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０
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３
６，Japan

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