MMS 研究会_共同研究レポート＜共同研究 4＞
研究題名： 小核試験におけるマウスの投与経路差の研究 (ip vs po)
研究成果： 投与経路の差は当時小核試験では一般的であった ip と実際の暴露経路に近い po に絞って比較検討がなされた．これまでと同様に定量的な差は認められたが，定性的な差は認められなかった．また，LD50 に対する割合で比較すると，定量的な差も小さくなった．近年，十分な暴露のために ip 投与の知見が要求される場合が認められたが，2017 年の IWGT の議論の結果，適切な理由がない限り，ヒトで妥当な暴露経路（po 投与など）を用いるべきとされた．
発表論文：
1)

Hayashi M, Sutou S, Shimada H, Sato S, Sasaki YF, Wakata A. Difference between intraperitoneal and oral gavage
application in the micronucleus test. The 3rd collaborative study by CSGMT/JEMS.MMS. Collaborative Study Group for theMicronucleus Test/Mammalian Mutagenesis Study Group of the Environmental Mutagen Society of Japan. Mutat Res. 1989
Aug; 223(4): 329-44.

2)

Hayashi M, Sofuni T, Kodama Y, Ishidate M, Tamura H. Micronucleus test with 1-β-d-arabinofuranosylcytosine administeredby intraperitoneal injection and oral gavage, Mutat Res. 1989 Aug; 223(4): 345-348.

3)

Hara T, Makita T, Horiya N, Ozawa S, Ohba M, Naito J, Shibuya T. Micronucleus test with 6-mercaptopurine monohydrate
administered intraperitoneally and orally, Mutat Res. 1989 Aug; 223(4): 349-352.

4)

Awogi T, Sato T. Micronucleus test with benzo[a]pyrene using a single peroral administration and intraperitoneal injection
in males of the MS/Ae and CD-1 mouse strains, Mutat Res. 1989 Aug; 223(4): 353-356.

5)Mizuhashi F, Murata K, Kitagaki T, Nishitomi T, Hashimoto A, Kido A. Administration-route-related difference in themicronucleus test with 7,12-dimethylbenz[a]anthracene, Mutat Res. 1989 Aug; 223(4): 357-360.

6)

Sugiyama C, Kobayashi H, Kishi M. Micronucleus test with 2-acetylaminofluorene by intraperitoneal injection and oraladministration, Mutat Res. 1989 Aug; 223(4): 361-364.

7)

Hachiya N, Aruga F, Aida M, Quan TH, Takizawa Y. Micronucleus induction in mouse bone marrow by phenacetin
administered intraperitoneally or orally, Mutat Res. 1989 Aug; 223(4): 365-368.
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MMS 研究会_共同研究レポート

8)

Wakata A, Yamashita T, Tamaoki M, Ohshima T, Kojima M, Micronucleus test with cyclophosphamide administered byintraperitoneal injection and oral gavage, Mutat Res. 1989 Aug; 223(4): 369-372.

9)

Kondo K, Suzuki H, Hoshi K, Yasui H. Micronucleus test with ethyl methanesulfonate administered by intraperitoneal
injection and oral gavage, Mutat Res. 1989 Aug; 223(4): 373-375.

10) Sutou S, Sato S, Hitotsumachi S, Kimura Y. Administration-route-related difference in the micronucleus test with N-ethylN-nitrosourea, Mutat Res. 1989 Aug; 223(4): 377-381.
11) Tsuyoshi T, Takeuchi M, Hirono H, Masamoto Y. Micronucleus test with methyl methanesulfonate administered byintraperitoneal injection and oral gavage, Mutat Res. 1989 Aug; 223(4): 383-386.
12) Sato S, Inui N, Ikeda Y, Hiraga Y. A comparison of intraperitoneal injection and oral gavage in the micronucleus test withmitomycin C in mice, Mutat Res. 1989 Aug; 223(4): 387-390.
13) Asano N, Morita T, Watanabe Y. Micronucleus test with colchicine given by intraperitoneal injection and oral gavage, Mutat
Res. 1989 Aug; 223(4): 391-394.
14) Ohuchida A, Furukawa A, Kondo Y, Ono T, Nito S, Micronucleus test with vincristine administered by intraperitoneal
injection and oral gavage, Mutat Res. 1989 Aug; 223(4): 395-398.
15) Nakajima M, Kitazawa M, Oba K, Kitagawa Y, Toyoda Y. Effect of route of administration in the micronucleus test withpotassium bromate, Mutat Res. 1989 Aug; 223(4): 399-402.
16) Shindo Y, Toyoda Y, Kawamura K, Kurebe M, Shimada H, Hattori C, Satake S. Micronucleus test with potassiumchromate(VI) administered intraperitoneally and orally to mice, Mutat Res. 1989 Aug; 223(4): 403-406.
17) Suzuki S, Atai H, Hatakeyama Y, Hara M, Nakagawa S. Administration-route-related differences in the micronucleus testwith benzene, Mutat Res. 1989 Aug; 223(4): 407-410.
18) Inoue T, Imanishi H, Watanabe M, Sasaki YūF, Shirasu Y, Ando N, Ishii S. Micronucleus test with procarbazine hydrochloride
administered by intraperitoneal injection and oral gavage, Mutat Res. 1989 Aug; 223(4): 411-414.

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