800-462-3417
For our catalog pdf
go to:
www.kendricklabs.com/pric.htm

Kendrick Labs Update
www.kendricklabs.com
Volume 2, Issue 1

Ask to be on ourmailing list:

August 2007

NEW! RADical approach to finding protein differences
between tissue, serum or organelle sample pairs.

♦ To be notified of
new developments
quickly.
♦To receive couponsand beta test offers.

Page 2 (over)
Examples of 2D gelsfrom RAD columns
Message fromJon Johansen,
Lab Manager

UNRAVEL YOUR
PR0TEOMIC
PROBLEMSWITH 2D GELSFOLLOWED BY
MASSSPECTROMETRY

Kendrick Labs Inc is offering RAD Technology in collaboration with GeneTel Labs (www.genetellab.com). GeneTel personnel will prepare chicken antibodies against your samples, generate reusable
antibody columns, and pass the sample(s) over the columns. Kendrick Labs will dialyze the samples,
measure protein concentration, run 2D gels, compare the patterns, and arrange for identification ofchanging proteins by mass spectrometry (MS). Price: $3600 for RAD, sample preparation, 2D gels/computer analysis, electronic images and complete report for 1 pair of samples. MS is additional.
Dr. David Huang of GeneTel Labs, who developed the RAD method (patent pending), has determinedthat protein depletion by the affinity columns depends on protein abundance and epitope number.
Mammalian proteins in high abundance are selectively removed by the chicken antibodies as well as
highly antigenic proteins in low abundance. The effluents from the reciprocal affinity columns containa complex mixture of proteins enriched for differences. Over for 2D gel images from an example:
David Huang, Ph.D.
GeneTel Labs LLC

Nancy Kendrick, Ph.D.
Kendrick Labs Inc

dhuang@genetel-lab.com

nancy@kendricklabs.com

877-248-4316

800-462-3417

2D gels from RAD and original samples
For our catalog pdf
go to:
www.kendricklabs.com/pric.htm

Kendrick Labs Inc
1202 Ann St
Madison, WI 53713
800-462-3417
Local: 608-258-1565
Fax: 608-258-1569

2d@kendricklabs.com
www.kendricklabs.com

Call or email fora price quote or
to discuss yourproject.

2 for 1
CouponFigure 1. 2D gel patterns from RAD and original samples from mammalian tissue homogenates(withpermission). The client has requested anonymity and non-disclosure of the tissue and disease pending
publication. Protein spots unique to or enriched in the control sample by eye are outlined in blue
while those unique to or enriched in the diseased sample are outlined in red. All differences wereconfirmed on duplicate 2D gels. Only 3 subtle differences were observed for the original samples,while 14 strong differences were observed for the RAD samples. A computerized comparison of theRAD 2D gels (duplicate gels/sample) is in progress.

Message from our Lab Manager, Jon Johansen:
To receive a free 2D gelwith your order (of at least 2gels) note NL:2-1 Coupon
on your sample ID form.
Includes silver staining but
not computer comparisons.
Expires 1/1/08.

Check out recent
references citing
Kendrick Labs
www.kendricklabs.com/
References-clients.htm

2D gels are especially useful for studying posttranslational modifications because they can
detect single charge changes. Evidence of this isshown below. GE Healthcare’s carbamylated
CPK pI standard was run on our 2D gels withand without SDS. The creatine phosphokinase
had been heated with urea buffer so that positively charged lysines were blocked one-by-one
by carbamylation. The charge isoforms resolve
well on 2D gels. The patterns +/- SDS are identical because SDS is completely stripped fromthe protein during isoelectric focusing.

Many people use Western blotting to detect new
charge isoforms. Western blotting is 10-100
times more sensitive than other staining methods
and gives beautiful results. Recently we havebeen focusing on phosphoprotein Western blotting using the PY20 antibody for P-Tyr and theQiagen Q5/Q7 antibodies for P-Ser and P-Thr.
See the links under “Western blots” on our web
page for details.
As always, call or email either me or Nancy todiscuss your project or for a quote. Our goal isto bring your project to a successful conclusion
so that you’ll tell your friends. Once we agree
on a project, Klabs will
hustle for you.
Jon Johansen, Lab Manager
Kendrick Labs Inc
800-462-3417
jon@kendricklabs.com