MMS 研究会_共同研究レポート＜共同研究 7＞
研究題名： IARC モノグラフ掲載の発ガン物質と小核試験の相関
研究成果： 本共同研究は海外のラボを巻き込んだ大規模なものとなり，論文作成までに多くの時間を要した．IARC でグループ 1, 2A, 2Bに分類された化合物で入手可能なものを探し，104 種類の被験物質について小核試験を実施し，試験法自体の性能を求めた．がん原性の判っている被験物質のみの検討であったので，検出力は評価できても精度等は対象外となった．ただし，小核試験の感度は低いと言われていた頃であり，ここで高い検出力を示せたことは小核試験に取って大きな意義があるものと考えることが出来る．また，化学物質の特性によって検出しやすいものと，検出が難しいもののあることも判明した．
発表論文：
1)

Morita T, Asano N, Awogi T, Sasaki YF, Sato S, Shimada H, Sutou S, Suzuki T, Wakata A, Sofuni T, Hayashi M. Evaluationof the rodent micronucleus assay in the screening of IARC carcinogens (groups 1, 2A and 2B) the summary report of the 6th
collaborative study by CSGMT/JEMS MMS. Collaborative Study of the Micronucleus Group Test. Mammalian Mutagenicity
Study Group. Mutat Res. 1997 Feb 28;389(1):3-122. Erratum in: Mutation Res., 391 (1997) 259-267. （Reference における文献番号表示）

2)

Kondoh Y, Nakajima E, Nito S, Asano Y, Ariyuki F, Higashikuni T. Assessment of the human carcinogens arsenic acid andarsenic trichloride in the mouse micronucleus assay, MMS Comm. 1994; 2: 117-121.

3)

Noguchi T, Asakura M, Sugiyama T, Matsushlma T. Asbestos (chrysotile) does not induce micronuclei in mouse bone marrowby intraperitoneal or intravenous injections, MMS Comm. 1994; 2: 75-77.

4)

Kondoh S, Sugiyama T. Melphalan is a potent in vivo clastogen as revealed by the mouse bone marrow micronucleus test,MMS Comm. 1994; 2: 97-98.

5)

Yamamura E, Hirono H, Takeuchi M. Nickel oxide(Ni2O3) did not induce micronuclei in mouse bone marrow cells, MMS
Comm. 1994; 2: 17-19.

6)Sugiyama C, Kobayashi H, Asano N, Ohuchida A, Kimura Y, Hitotsumachi S. In vivo and in vitro cytogenetic studies of foursex hormones, MMS Comm. 1995; 3: 1-13.
1

MMS 研究会_共同研究レポート

7)

Miyamae Y, Hirai O, Yamada H, Sakaguchi M, Sasaki YF, Kojima H, Sato A, Higashikuni N, Sutou S. The induction ofmicronuclei in mice by dimethyl sulfate and diethyl sulfate, MMS Comm. 1995; 3: 25-32.

8)

Satake K, Hayakawa Y, Watanabe M, Matsumura H, MatsumotoK, Ohta T. No induction of mouse bone marrow micronuclei
by 4,4'-methylene bis(2-chloroaniline), MMS Comm. 1994; 2: 15-16.

9)

Cui X, Higashikuni N, Sutou S. Comparative study of micronucleus induction by nitrogen mustard in the peripheral bloodand bone marrow of mice and rats, MMS Comm. 1995; 3: 69-75.

10) Higashikuni, N, Morita T, Sutou S. N-nitrosodiethylamine induces micronuclei at doses below the maximum tolerated dose,
MMS Comm., 1994; 2: 1-6.
11) Kimura Y, Hitotsumachi S. No induction of micronuclei by vinyl bromide in Mouse bone marrow cells, MMS Comm., 1994; 2:9-11.
12) Ozawa S, Kimura Y, Hitotsumachi S. Acetaldehyde induces micronuclei in mice administered intraperitoneally, MMS Comm.,
1994; 2: 33-35.
13) Miura D, Kasahara Y, Morita K, Nakai Y, Hirabayashi K, Wada H, Takahashi Y, Izawa Y, Makita T. Acetamide induced
neither bacterial gene mutations nor micronuclei in mice, MMS Comm., 1994; 2: 49-57.
14) Higashikuni, N, Hara M, Nakagawa S, Sutou S. 2-(2-furyl)-3-5-(5-nitro-2-furyl) acrylamide (AF-2) is a weak in vivo clastogenas revealed by the micronucleus assay, Mutat, Res. 1994 ;320: 149-156.
15) Asanami S, Shimono K, Uejima M, Yamamura E, Hirono H, Takeuchi M. Mouse micronucleus test of β-butyrolactone and βproppiolacltone, MMS Comm., 1994; 2: 109-116.
16) Shimada Y, Ozawa S. 4-chloro-o-phenylenediamine is clastogenic in the mouse bone marrow micronucleus test, MMS Comm.,
1994; 2: 25-28.
17) Sasaki YF, Sakaguchi M, Yamada H. Evidence for non-clastogenicity to mice of possible human carcinogen (IARC) p-cresidine,MMS Comm., 1994; 2: 83-85.
18) Kondoh S, Sugihara T. No induction of micronuclei in mice after intraperitoneal injection of 2,4-diaminoanisole
2MMS 研究会_共同研究レポート

dihydrochloride, MMS Comm., 1994; 2: 95-96.
19) Sasaki YF, Sakaguchi M, Yamada H, Matsuhashi T. Evaluation of micronucleus induction in mice by four organochlorine
pesticides: 1,2-dlbromo-3-chloropropane, 1,3-dichloroprope, 1,2-dichloroethane, and nitrofen, MMS Comm., 1994; 2: 87-93.
20) Ozawa S. No clastogenicity of dichloromethane in the mouse bone marrow as administered intraperitoneally, MMS Comm.,1994; 2: 29-31.
21) Ohuchida A, Kouchi Y, Sugiyama C, Kobayashi H, Atai H, Y. Hatakeyama Y. The in vivo mutagenic potential of hydrazineand its derivatives, MMS Comm., 1995; 3: 15-23.
22) Morita T. No clastogenicity of 1,4-dioxane as examined in the mouse peripheral blood micronucleus test, MMS Comm., 1994;
2: 7-8.
23) Suzuki Watanabe C, Nakae S. 1,2.3,4,5,6-Hexachlorocyclo-hexane does not induce micronucleus in mice, MMS Comm., 1994;
2: 41-43.
24) Honda S, Hayashi M, Shinagawa Y, Sato S, Naruse Y, Kagamimori S. Methylthiouracil does not induce micronuclei in mousereticulocytes, MMS Comm., 1994; 2: 37-40.
25) Suzuki T, Hayashi M, Honma M, Sofuni T. Micronuccleus induction by monocrotaline in mouse peripheral blood, MMS Comm.,
1995; 3: 81-85.
26) Sato S, Takeuchi M. Cytogenetic effects of 2-nitropropane in in vitro and in vivo, MMS Comm., 1994; 2: 123-127.
27) Satake K, Hayakawa Y, Watanabe M. N-nitrosodiethanolamine is not a clastogen as revealed by the mouse bone marrow
micronucleus test, MMS Comm., 1994; 2: 13-14.
28) Miyamae Y, Hirai O. No induction of micronuclei in the peripheral reticulocytes of N-nitrosopiperidine-treated mice, MMSComm., 1994; 2: 45-47.
29) Noguchi T, Asakura M, Sugiyama T, Matsushima T. N-Nitoroso-di-n-propylamine induces micronuclei in partiallyhepatectomized rat liver but not in mouse bone marrow cells, MMS Comm., 1994; 2: 79-82.
30) Shimada. Y, Ono S, Kobayashi S, Cui X, Sutou S. No micronucleus induction by phenobarbital sodium as revealed in mouse3MMS 研究会_共同研究レポート

bone marrow and peripheral blood tests, MMS Comm., 1995; 3: 61-67.
31) Yamamura E, Hirono H, Takeuchi M. 1,3-Propane sultone-induced micronuclei in mouse peripheral blood, MMS Comm.,1994; 2: 21-23.
32) Nakai, Y, Hirabayashi K, Takahashi Y, Miura D, Kasahara Y, Moruta K and Izawa Y. The genetic toxicology of o-toluidinewith special reference to its non-clastogenicity in vivo, MMS Comm., 1994; 2: 99-108.
33) Sato S, Taketomi M. The mouse micronucleus test with formaldehyde, MMS Comm., 1994; 2: 69-73.

4