Calcium Flux Assay Services
with hiPSC-derived Neurons Generated by Quick-TissueTM technology
Applications
Neuronal Characterization
Disease Phenotyping
Neurotoxicity Screening
Electrophysiological Assay with Calcium Flux Assays
Calcium is an essential intracellular messenger in neurons, generating a multitude of intracellular signals that control key
neuronal functions. Calcium Flux assays offer a useful platform for investigating a variety of functional characteristics ofneurons in a high-throughput manner. hiPSC-derived neurons generated by our Quick-TissueTM Technology show
strong and consistent synchrony, which is suitable for Calcium Flux assays.

Advantages of Working with Elixirgen Scientific
1. One-Stop Services: We do more than just Assays
Projectdesign

Human
iPSCs

iPSC
expansion

Qualitycontrol

Differentiation

AssayNo need to work with multiple service providers! We offer differentiation services with assay services. Assays can be
performed with neurons differentiated from customer iPSCs including disease state iPSCs.

2.

Stable assays with a novel coating method
CV0.39

CV
0.42

CV CV
0.37 0.37

Plate1
Plate2

Co-cultured withprimary astrocytes

Co-cultured withiPSC-derived astrocytes

Ricoh’s coating
Conventional coating
Ricoh has developed a novel coating method that enables better cell adhesion to the plate and less aggregation compared tothe conventional methods. The improved culture conditions offer stable Calcium ﬂux assays with small well-to-well andplate-to-plate variability. Quick-Neuron™ Excitatory Neurons were co-cultured with human primary astrocytes or
Quick-Glia™Astrocytes (iPSC-derived astrocytes) on 384-well plates for 6 weeks post-thaw and used for Calcium Flux assays.
Web ● www.elixirgensci.com ∣ Phone ● +1 (443) 869-5420 ∣ Email ● info@elixirgensci.com

Calcium Flux Assay Services
Key Components of Calcium Flux Assay Services

Cells

CalciumFluxAssayAnalysis

Our standard
Human iPSC-derived excitatory neurons from a healthy control line (EX-SeV-CW50065)
co-cultured with human iPSC-derived astrocytes from a control (AS-SeV-CW50065)
Options●
Other neuron subtype: Cholinergic neurons (motor neurons)●
Other iPSCs from CIRM iPSC repository: Alzheimer’s disease, Epilepsy, Autism,etc.
●Starting from customer iPSC lines is also an option.
Our standard
FDSS/μCELL (Hamamatsu Photonics) + 384-well plate
10 compounds at 4 doses with n=6 biological replicates(240 wells per one 384-well plate)
Recording 20 min before and after test compound administration
Options
FDSS/μCELL (Hamamatsu Photonics) + 96-well plate
5 compounds at 2 doses with n=6 biological replicates(60 wells per one 96-well plate)
Our standard
Ca2+ spike number, base amplitude, Ca2+ spike amplitude,
Ca2+ spike duration, Ca2+ spike area, spike to spike time
Options
Other secondary analysis may be possible upon requestLog (Change ratio of spike
number (after/before))Eﬀect of Various Compounds on Number of Ca2+ Spikes(Na+ channel blocker)(K+ channel blocker)(Glu R antagonist)(GABA R agonist)(Antiepileptic agent)

Human iPSC-derived neurons co-cultured with human primary astrocytes responded to various neurotransmitters, receptor
antagonists and ion channel blockers as expected.

We oﬀer multiple services that can be combined with
Calcium Flux Assays. Contact us to request a quote or aconsultation.

Check out
our website formoreinformation!

Web ● www.elixirgensci.com ∣ Phone ● +1 (443) 869-5420 ∣ Email ● info@elixirgensci.com