Immunoassays
Luminex Portfolio

R&D Systems™
Luminex Portfolio®

Instruments, Assays, and Software

R&D Systems™ has acomplete solution to
optimize productivity
across your Luminex®
workflow.

Luminex Instruments and R&D
Systems Assays
From screening biomarkers for further evaluation to validating resultsfor drug development, multiplex immunoassays offer several critical
performance advantages. The ability to measure multiple targets in asingle assay allows researchers to detect hundreds of analytes in a single
run while being judicious with precious sample volumes.
As one of the most trusted platforms for high-level protein multiplexing,
R&D Systems Luminex assays are designed to maximize multiplexing
capacity and flexibility while maintaining assay specificity. Utilizing
Luminex xMAP® microparticle technology, Luminex assays from R&D
Systems are rigorously validated for accuracy, reproducibility, and lot-tolot consistency.

Luminex Assays by R&D Systems
R&D Systems Luminex Assays are ideal for researchers needing toevaluate multiple analytes for trends in protein expression in diseasestates or following treatment. Validated and tested for optimum
performance, R&D Systems Luminex Assays are designed to optimize
multiplexing capabilities while delivering consistent and accurate results.
• Choose from a rapidly expanding menu of over 450 analytes• Select kits designed for human, mouse, rat, non-human primate, andporcine analytes• Run a multiple analyte assay in 3–3.5 hours• Assay multiple analytes in a small sample volume (< 50 μL)• Choose from three flexible formats

Validation and Testing
Antibody pairs are selected and tested to confirm the parallel detectionof natural and recombinant standard protein and to ensure the accurate
determination of target analyte within biological samples
R&D Systems Luminex Assays are validated for use with cell culture
supernatants, plasma, or serum.
Assays for each target analyte are screened against all target analytes toconfirm low antibody cross-reactivity.

02

B I O -T E C H N E ®

// R & D S Y S T E M S L U M I N E X P O R T F O L I O

03

Luminex Instrumentation
Luminex instruments offer proven precision and reliability, making themamong the most widely used multiplexing platforms in the market. Usinga mixture of color-coded beads pre-coated with capture antibodies,
powerful xMAP technology offers the ability to bind up to 50 different
analyte targets in solution. Upon completion of an R&D Systems Luminex
assay, beads are analyzed with a dedicated dual-laser flow-based
detection instrument. From the Luminex 200 to the advanced xMAPINTELLIFLEX, choose the Luminex instrument that best meets your uniqueapplication requirements.

Find the Luminex
multiplexing systemperfect for yourlaboratory’s needs.

TA B L E // 0 1

Luminex Instrument
Luminex 200
Instrument System

Luminex FLEXMAP
3D Instrument
SystemLuminex xMAPINTELLIFLEX
SystemLuminex xMAPINTELLIFLEX
DR-SE System

Up to 100

Up to 500

Up to 500

Up to 500

Read time for
96 Well Plate~40 minutes~20 minutes~20 minutes~20 minutes

Read time for
384 Well Plate

N/A~75 minutes~75 minutes~75 minutes

Dynamic Range

3.5 logarithmicunits

4.5 logarithmicunits≥5.5 logarithmicunits≥5.5 logarithmicunits

Microtiter Wells

96

96 and 384

96 and 384

96 and 384

Dimensions(W x D x H)

25.3” x 23.5” x 12.5”
64cm x 60cm x
32.5cm23” x 25.7” x 18”
58.4cm x 63.5cm x
45.7cm23” x 24” x 30”
58.4cm x 61cm x
76.2cm23” x 24” x 30”
58.4cm x 61cm x
76.2cmTouchscreen

N/A

N/A

Yes

Yes

Automated Startup

N/A

N/A

Yes

Yes

532 nm (green)

532 nm (green)

532 nm (green)

532 nm (green) and
405 nm (violet)

Dual Reporter Readout

N/A

N/A

N/A

Yes

Barcode Reader for
Calibration andVerification Reagents

N/A

N/A

Yes

Yes

Number of Targets
Multiplex Capacity

Reporter Laser

04

B I O -T E C H N E ®

Quantist Luminex data analysis software helps you
extract valuable insights from data complexity.
Use this software to evaluate the quality of yourstandards, controls, and unknowns. Evaluate thelong-term consistency of your Luminex data by using
Quantist to make inter-assay comparisons. Import
CSV files from any Luminex instrument and export
ready-to-graph, Excel files.
• Easily exclude individual wells or aggregates

TA B L E // 0 2

Quantist Minimum
Software Requirements

Processor

3.0 GHz i7 6th gen

Ram

8 GB

Hard Drive

256 GB

Ports

1-USB 1-NIC

OS

Windows 10 Pro 64bit

Screen Resolution

1600x900 24 bit color// R & D S Y S T E M S L U M I N E X P O R T F O L I O

Quantist Software• Remove low bead counts with a single click• Assign or update well assignments in the Luminex
96‑well plate• Export optimized data for graphing

Try free for 30 days | bio-techne.com/reagents/luminex/luminex-software-quantist05

NEW Luminex xMAPINTELLIFLEX
The xMAP INTELLIFLEX system is an advanced
multiplexing platform that delivers accurate andreliable protein measurement results. Featuring
a second reporter laser (405 nm) alongside thetraditional reporter channel, the INTELLIFLEX systemenables the detection of two parameters on a single
bead, allowing you to maximize your data points andthe number of samples you can process. Designed
for a 96- or 384-well high throughput format, the
INTELLIFLEX system can save substantial timecompared to a single-plex or lower-throughput assay

INTELLIFLEX Features.
• 5.5 logarithmic units of dynamic range
Flexibility for multiplex targets minimizes theneed to dilute samples to fall within the rangeof the assay.
• Integrated touchscreen PC
Integrated touchscreen enables space savings andconvenient and intuitive setup.

• Built-in barcode reader
Faster setup and convenient tracking system,
particularly for large experiments.
• Automated startup, shutdown andmaintenance routines
Increase your productivity withwalk-away routines.

xMAP INTELLIFLEX
DR-SE System
All features of the xMAP INTELLIFLEX System plus:• Dual reporter system
Second violet (405 nm) laser allows you tomonitor two parameters per analyte.
• Side-eject door
Enables end-to-end automation to
maximize throughput.

06

B I O -T E C H N E ®

Intelliflex System
xMAP® INTELLIFLEX RUO

xMAP® INTELLIFLEX DR-SE RUO®

INTELLIFLEX-RUOINTELLIFLEX-DRSE-RUO

Multiplexing Capability

Up to 500 individual analytes per well

Up to 500 individual analytes per well;
Up to 2 parameters per analyte

Typical Dynamic Range≥5.5 logs≥5.5 logs≥4.5 logs (second reporter channel*)Sensitivity

Commercially availalbe kits deliver sensitivity as low as sub-picogram level

Part Number
Performance// R & D S Y S T E M S L U M I N E X P O R T F O L I O

TA B L E // 0 3

GeneralWeight54.4 kg (120lbs)

System Warm-Up

30 minOperating Temperature

15-30°C (59-85°F)

Operating System

Microsoft Windows 10 IoT Enterprise LTSC

Barcode Reader

For importing target values from the xMAP INTEILLIFLEX Calibration and Performance
Verification Kits

Reagents Included

xMAP® INTELLIFLEX Performance Verification Kit
xMAP® INTELLIFLEX Calibration Kit
xMAP® Sheath Fluid PLUS, 20 L

Fluidics
Sheath Pressure

8-13 psi

Sample Injection Rate

2 μL/sec

Sample Uptake Volume

10-200 μL

OpticsLasers

1 Classification laser
1 Reporter laser

Classification Laser
Excitiation

638 nmReporter Laser 1(RP1) Excitation

532 nmReporter Laser 2(RP2) Excitation*n/a

RP1 Detector

565-589 nm

RP2 Detectorn/a

1 Classification laser
2 Reporter lasers*

405 nm421-441 nm

The second reporter channel is only available in thexMAP INTELLIFLEX DR-SE RUO model.
*

07

Luminex 200
The Luminex 200 is the most popular installed
instrument for basic multiplexing protein needs.
• Simultaneously multiplex up to 100 analytes in asingle microplate well
• Delivers fast and cost-effective bioassay results
on many assay formats• Calibrated using a calibration and averification kit, each providing 25 uses

TA B L E // 0 4

LUMINEX 200
Luminex 200 System with xPONENT 4.3

08

Part Number

LX200-XPON-RUO

Core Instrument

Luminex 200 flow-based bead reader with: Sheath Delivery System; X-Y Platform
for 96-well plates; Sample probe-needle; 1L sheath bottle, 1.2L Waste bottle;
Sample probe height adjustment kit; Automated Maintenance Plate; Heater Block(96 well plate); Documentation

Available Power Cords(pick one)

USA, Australia, Brazil, Denmark, Germany, Sweden, France, Belgium, Spain,
Switzerland, Israel, UK, Italy, Japan, India,China, S. Africa, Argentina,
Korea, Taiwan

Barcode Scanner

Optional, at no charge with purchase of new system

Software Included

xPONENT 4.3 (3 seats)
Allele-call module (3 seats)
Remote Web Monitoring module (3 seats)

Optional Software(extra charges apply)

LIS integration module
Automation interface module
Basic Security Module
21 CFR Part 11 Module (supersedes Basic Security)
Additional seats

Reagents Included

LX2R-CAL-K25, qty 1 (separately shipped)
LX2R-PVER-K25, qty 1 (separately shipped)
40-50021, xMAP Sheath Fluid (20L), qty 1 (ships with LX200)

Desktop PC with WIN 10 64-bit,
US English & xPONENT 4.3

Dell Optiplex with 2 Port PCI RS232 Serial Adapter Card
Dell Warranty Transfer
Dell 17” LCD monitor with soundbar
Dell Keyboard & mouse

B I O -T E C H N E ®

The FLEXMAP 3D is an advanced and highly versatile
multiplexing platform. Fast read time, compatibility
with 96- and 384-well plates, and ease of integration
with front-end automation systems make the
FLEXMAP 3D the platform of choice for highthroughput applications.
• Multiplexing: Up to 500 analytes in a single
sample volume// R & D S Y S T E M S L U M I N E X P O R T F O L I O

FLEXMAP 3D®

• Dynamic Range (Typical): >4.5 logs
• Read Time: 96-well plate in ≤ 20 min (up to
144,000 tests/hour); 384-well plate in ≤ 75 min (upto 153,600 tests/hour)• Daily Start-Up/Shut-Down: ≤ 30 min
TA B L E // 0 5

FLEXMAP 3D
FLEXMAP 3D xPONENT 4.3 System
Part Number

FLEXMAP-3D-RUO

Core Instrument

FLEXMAP 3D flow based bead reader with: Sample probe-needle; Off plate
reagent block; Waste container; Sample probe height adjustment tool; Barcode
Scanner; Heater Block 96-well; Heater Block 386- well; Documentation

Available Power Cords(pick one)

USA, Australia, Brazil, Denmark, Germany, Sweden, France, Belgium, Spain,
Switzerland, Israel, UK, Italy, Japan, India, China, S. Africa, Argentina, Korea,
Taiwan, Pakistan

Software Included

xPONENT 4.3 (3 seats),
Allele-call module (3 seats)
Remote Web Monitoring module (3 seats)

Optional Software(extra charges apply)

LIS integration module
Automation interface module
Basic Security Module
21 CFR Part 11 Module (supersedes Basic Security)
Additional seats

Reagents Included

F3D-CAL-K25, qty 1 (separately shipped)
F3D-PVER-K25, qty 1 (separately shipped)
40-50021, xMAP Sheath Fluid (20L), qty 1 (Ships with FLEXMAP 3D)

Desktop PC with Win 10 64-bit,
US English

PC, Desktop, USFF, WIN 10 with xPONENT 4.3
Microsoft® Windows® 10 Professional 64 bit, version 1709 or greater
Dell Warranty Transfer
Arm Mounted ELO 19” Touch Screen Monitor
AX2010 USB Stereo Speaker System
Keyboard & Mouse

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TA B L E // 0 6

xMAP® Instrument Reagents
Reagent

Pack Size

Part Number

MAGPIXMAGPIX Drive Fluid, 4 × 750 mL containers
MAGPIX Calibration Kit
MAGPIX Performance Verification Kit

4 pack
25 uses
25 uses40-50030
MPX-CAL-K25
MPX-PVER-K25

Luminex 100/200(all versions)

Sheath Fluid
Sheath Concentrate Pack (20x)

20 L
1L

40-50021
40-50023

Luminex 100/200(xPONENT only)

Luminex 100/200 Calibration Kit
Luminex 100/200 Performance Verification Kit

25 uses
25 uses

LX2R-CAL-K25
LX2R-PVER-K25

FLEXMAP 3D

Sheath Fluid
Sheath Concentrate Pack (20x)
FLEXMAP 3D Calibration Kit
FLEXMAP 3D Performance Verification Kit

20L
1L
25 uses
25 uses40-50021
40-50023
F3D-CAL-K25
F3D-PVER-K25

INTELLIFLEXxMAP® INTELLIFLEX Performance Verification Kit
RUO
xMAP® INTELLIFLEX Calibration Kit RUO
xMAP® Sheath Fluid PLUS RUO
xMAP® Sheath Concentrate PLUS

20 uses
20 uses
20L
1L

IFX-PVER-K20
IFX-CAL-K20
40-50021
40-50023

Magnetic Plate Separator
The Luminex Magnetic Plate Separator, LU-MAG-01,
is a modified plate magnet intended to separate a
96-well plate of MagPlex Microspheres during assay
preparation steps. The side clasps on this magnet
have been specially designed to hold a microplate
securely to the magnet, enabling the user to quickly

10

invert and decant it over a waste container. Similar
to the method used in an ELISA, this manual plate
washing method has been shown to effectively
remove nearly all of the supernatant from every wellon the plate, with virtually no bead carry-over.

B I O -T E C H N E ®
// R & D S Y S T E M S L U M I N E X P O R T F O L I O

TA B L E // 0 7

Instrument Service Plans
Luminex 200, FLEXMAP 3D, and INTELLIFLEX
Number of visits for emergency repair

Unlimited

Emergency repair costs included in plan

All Costs Included

Preventative maintenance visits per year

1–2

On-site response time (business days)

1–2

Priority scheduling for service requests

Included

Product updates and hardware modifications

Included

Software support

IncludedUnlimited 24 ×7 × 365 remote support

Included (Unlimited, during business hours - Japan)

On-site assay support, as needed(Luminex Assays Only)

Not Included

Learn More | bio-techne.com/instruments/luminex

Bio-Techne Academy
The Luminex Learning Plan at the Bio-Techne
Academy has all the training you need toget up andrunning with your Luminex instrument, assays, andQuantist Analysis software. The course includes
modules on Luminex technology, installation,ordering and running R&D Systems assays, how toanalyze data, instrument maintenance protocols andtroubleshooting. Align the team with courses ranging
from a quick refresher to new employee training.

ACADEMYTHE ONLINE EXPERIENCE

Self-register to get more! | academy.bio-techne.com/learn

11Luminex Assay
Offerings

Ordering Your R&D Systems
Luminex Assays
The R&D Systems Luminex Assay Online Ordering Tool is designed to help
you choose the right Luminex Assay for your unique research needs.
• Save your selected Luminex Assay configuration for easy re-ordering• View how the selection of each assay specification changes the final
kit options• Find pricing options for premixed or user mixed High
Performance Assays• Alter your assay selections at any time with the tool’s easy navigation• Get access to ordering or technical support via phone and email
Ordering assays online is easy | bio-techne.com/luminex-assay-customization-tool

Custom Luminex Services
Employ our assay experts and our extensive in-house collection of
antibodies and proteins to build your specific custom multiplex panel.
Custom services may include performance optimization of analytes fromour menu of over 450 analytes, or the creation of an assay for a biomarker
that we don’t currently offer in any product categories, or even theevaluation of specific sample types that our kits were not initially tested
for. Expedite your project by putting our experience and reagents to workfor you.
• Create multiplex assays for existing or new analytes• Develop assays for your unique specifications• Validate for your specific sample types• Supply agreements available
Learn more online | bio-techne.com/services/custom-luminex-services

12

B I O -T E C H N E ®

R&D Systems Luminex
High Performance Assays

Personalize your multiplex immunoassay panel using
Luminex Discovery Assays. Create your panel of up
to 50 analytes with the largest customizable menu
on the market. Explore our large catalog of human,
mouse, rat, and porcine targets. All assays can be
performed in 3.5 hours or less.

Correlated with the gold standard Quantikine™ ELISA
kits, our Luminex High Performance Assay panels
are optimized with custom diluents and extensively
validated to ensure consistent and accurate results.
Pre-defined multiplex panels are designed using key
analytes from chosen research areas.

• Most flexible panel building on the market• Our most accurate and precise bead-based
multianalyte profiling kits.

• Custom built panels of up to 50 analytes• Panels come pre-mixed for easy set-up• Validated and tested for optimum performance104// R & D S Y S T E M S L U M I N E X P O R T F O L I O

R&D Systems Luminex
Discovery Assays• High sensitivity panels available for low
abundance proteins.
• Large XL Cytokine panels for detecting 38-46
analytes.
• Flexible panel options from configurable premixed
kits, user mix, and off‑the‑shelf fixed panels

CXCL16 Standard
Natural CXCL16
103

MFI

Unstimulated vs. Stimulated PBMCs

140,000

101
1

101

102

103

pg/mL

102

Unstimulated (before)
Stimulated (after)

100,000

60,000CXCL16 (pg/mL)
20,000Figure 1. Luminex Assays undergo testing to ensure that theydetect natural proteins in parallel with the recombinant protein
standard. A dilution curve detecting natural or recombinant
standard CXCL16 is shown. Parallel detection of natural andrecombinant standard proteins ensure that the concentration
within a biological sample can be accurately determined incomparison to the recombinant protein used to generate thestandard curve.
Learn More | bio-techne.com/reagents/luminex-assays/
luminex-discovery-assays

0
IFNg

TNFa

IL-10

IL-1raMIP-1a MIP-1B

IL-1raAnalyteFigure 2. The Human XL Cytokine Performance Panel,
FCSTM18B, detects the inflammatory response of CytokineRelease Syndrome (CRS) biomarkers. Unstimulated andstimulated PBMCs were tested for multiple analytes to measure
the different levels that are seen in CRS. The Human XL Cytokine
Performance Panel’s high sensitivity and superior accuracy
offers the most reliable method of measuring CRS-associatedinflammatory cytokines.
Learn More | bio-techne.com/reagents/luminex-assays/
luminex-high-performance-assays

13

TA B L E // 0 8

Choose Your R&D Systems
Luminex Assay Format
Luminex Discovery Assay

Species Available

Luminex High
Performance Assay

Human

✓

✓

Mouse

✓

✓

Rat

✓
✓

PrimatePorcine✓Sample Volume RequiredµL Sample≤ 25 - 50≤ 25 - 50

Multiplex Analyte
Capacity

Magnetic

50 User-Selected

User-Selected from Panel,or Fixed Panel

Serum

✓

✓

Plasma✓✓Cell Culture Supernates✓

See Datasheet**

Validated Sample Types

Run Time
Kit Preparation

Assay Validation

Analytes Available*

See Datasheet**

SalivaSee Datasheet**Urine

See Datasheet**

Hours

3–3.5

2.5–5

✓

✓

Premixed✓

End-user Mixed
Mean Sensitivity*< the low standard≤ 3/4 the low standard

Intra-assay Precision

< 20%

< 15%

Intra-assay Precision

< 30%

< 17%

Recovery(Individual Samples)

N/A

70–130%

Assay Linearity

Dose-Dependent
Decrease in Values

1:2–1:8

Linearity(Individual Samples)

Dose-Dependent
Decrease in Values

70–130%

Human

> 350

>135

Mouse

> 108

45

Rat

17

0

Primate036

Porcine20

0

Mean sensitivity details | bio-techne.com/luminex-assay-customization-tool

**

14

Milk

For more information | bio-techne.com/reagents/luminex-assays/luminex-high-performance-assays

B I O -T E C H N E ®

// R & D S Y S T E M S L U M I N E X P O R T F O L I O

15TA B L E // 0 9

R&D Systems Luminex® High Performance
Assays Fixed Panels
Human Cytokine Panel 15-Plex, LKTM011B
IFN-alphaIL-1 beta/IL-1F2

IL-3

IL-7

IL-15

IFN-gamma

IL-1ra/IL-1F3

IL-4

IL-9

IL-33

IL-1 alpha/IL-1F1

IL-2

IL-6

IL-10

VEGF

Human Chemokine Panel 8-Plex, LKTM012B
CCL2/JE/
MCP-1

CCL4/MIP-1 beta

CCL11/Eotaxin

CXCL10/IP-10/
CRG-2

CCL3/MIP-1
alpha

CCL5/RANTESCXCL1/GRO alphaIL-8/CXCL8Human Growth Factor Panel 21-Plex, LKTM013B
CCL3/MIP-1
alpha

EGF

Granzyme B

IL-9

PDGF-AA

CCL19/MIP-3
beta

FGF basic/FGF2/bFGF

IFN-beta

IL-17E/IL-25

PDGF-AB/BB

CD40 Ligand/
TNFSF5

Flt-3 Ligand/FLT3L

IL-3

IL-33

TGF-alpha

CXCL2/GRO
beta

G-CSF

IL-8/CXCL8

PD-L1/B7-H1

TRAIL/TNFSF10

VEGF

Human Immunotherapy Panel 25- Plex, LKTM010B
CCL2/JE/
MCP-1

GM-CSF

IL-1 beta/IL-1F2

IL-8/CXCL8

IL-15

CCL3/MIP-1
alpha

Granzyme B

IL-1ra/IL-1F3

IL-9

IL-17/IL-17A

CCL4/MIP-1
beta

IFN-alpha

IL-2

IL-10

IL-33

CD40 Ligand/
TNFSF5

IFN-gamma

IL-4

IL-12 p70

PD-L1/B7-H1

CXCL10/IP-10/
CRG-2

IL-1 alpha/IL-1F1

IL-6

IL-13

TNF-alpha

TNF-beta

Human Th1/Th2 Panel 13-Plex, LKTM008B
GM-CSF

IL-2

IL-6

IL-12 p70

IFN-gamma

IL-4

IL-9

IL-13

IL-1 beta/IL-1F2

IL-5

IL-10

TNF-alpha

Human Th9/Th17/Th22 Panel 18-Plex, LKTM009B

16

CCL20/MIP-3
alpha

IFN-gamma

IL-4

IL-9

IL-13

IL-17E/IL-25

CD40 Ligand/
TNFSF5IL-1 beta/IL-1F2

IL-5

IL-10

IL-15

IL-33

GM-CSF

IL-2

IL-6

IL-12 p70

IL-17/IL-17A

TNF-alphaB I O -T E C H N E ®

R&D Systems Luminex® High Performance
Assays Fixed Panels Continued
Human TIMP Panel 4-Plex, LKTM003
TIMP-1

TIMP-2

TIMP-3

TIMP-4

Human XL Cytokine Panel 46-Plex, LKTM014B
CCL2/JE/
MCP-1

CXCL1/GRO alphaGranzyme B

IL-3

IL-12 p70

PDGF-AB/BB

CCL3/MIP-1
alpha

CXCL2/GRO beta

IFN-alpha 2/IFNA2

IL-4

IL-13

TGF-alphaCCL4/MIP-1
beta

CXCL10/IP-10/
CRG-2

IFN-beta

IL-5

IL-15

TNF-alphaCCL5/RANTES

EGF

IFN-gamma

IL-6

IL-17/IL-17A

TNF-beta

CCL11/Eotaxin

FGF basic/FGF2/
bFGF

IL-1 alpha/IL-1F1

IL-7

IL-17E/IL-25

TRAIL/TNFSF10

CCL19/MIP-3
beta

Flt-3 Ligand/FLT3L

IL-1 beta/IL-1F2

IL-8/CXCL8

IL-33

VEGF

CCL20/MIP-3
alpha

G-CSF

IL-1ra/IL-1F3

IL-9

PD-L1/B7-H1

CD40 Ligand/
TNFSF5

GM-CSF

IL-2

IL-10

PDGF-AA// R & D S Y S T E M S L U M I N E X P O R T F O L I O

TA B L E // 0 9

Mouse XL Cytokine Panel 45-Plex, LKTM015
BAFF/BLyS/TNSFS13B

CXCL1/KC

I-CAM

IL-5

IL-16

LIX

CCL2/JE/
MCP-1

CXCL10/IP-10/
CRG-2

IFN-gamma

IL-6

IL-17A

M-CSF

CCL3/MIP-1
alpha

EGF

IL- 1 alpha/IL-1F1

IL-7

IL-18

TIMP-1

CCL4/MIP-1
beta

FGF-basic

IL-1 beta/IL-1F2

IL-9

IL-21

TNF-alphaCCL5/RANTES

Flt-3 ligand

IL-1ra/IL-1F3

IL-10

IL-27

VEGF

CCL11/Eotaxin

G-CSF

IL-2

IL-11

IL-31

CCL19/
MIP-3beta

GDF-15

IL-3

IL-12 p70

LDL R

Chitinase3-like 1

GM-CSF

IL-4

IL-13

LIF

17

TA B L E // 1 0

R&D Systems Luminex®
High Performance Assays
Human Biomarker Panel A, Premix FCSTM13, User Mix LBAM000
BAFF/BLyS

CD14

CXCL13/BLC/
BCA-1

IL-2 Ra

CCL20/MIP-3
alpha

CD27/TNFRSF7

gp130

CD25/IL-2R alpha

TNF RII/TNFRSF1B

Human Cytokine Panel A, Premix FCSTM03, User Mix LUHM000
CCL2/MCP-1

CXCL5/ENA-78

GM-CSF

IL-1ra/IL-1F3

IL-6

Thrombopoietin/Tpo

CCL3/MIP-1 alphaCXCL8/IL-8

IFN-gamma

IL-2

IL-10

VEGF

CCL4/MIP-1 beta

FGF basic

IL-1 alpha/IL-1F1

IL-4

IL-17

CCL5/RANTES

G-CSF

IL-1 beta/IL-1F2

IL-5

TNF-alpha

Human High Sensitivity Cytokine Panel A, Premix FCSTM09, User Mix LHSCM000
CXCL8/IL-8

IFN-gamma

IL-2

IL-5

IL-10

TNF-alpha

GM-CSF

IL-1 beta/IL-1F2

IL-4

IL-6

IL-12 p70

VEGF

Human High Sensitivity Cytokine Panel B, Premix FCSTM14, User Mix LBHS000
GM-CSF

IL-2

IL-7

IL-17A

IL-23

IL-36 beta

IFN-g

IL-5

IL-13

IL-17F

IL-31

TNF-alpha

IL-1b

IL-6

IL-15

IL-22

IL-33

Human Immuno-Oncology Panel Performance Panel, Premix FCSTM24
CCL17/TARC

CD40/TNFRSF5CXCL5/ENA-78

LAG-3

PD-L2/B7-DC

CD27/TNFRSF7

CD25/IL-2R alpha

HVEM/TNFRSF14

OX40 Ligand/
TNFSF4

TIM-3

CD28

CTLA-4

IFN-gamma

PD-L1/B7-H1

Human Kidney Biomarker Panel, Premix FCSTM16, User Mix LHK000
Clusterin

CXCL10/IP-10/
CRG-2

Cystatin C

Lipocalin-2/NGAL

Osteopontin
(OPN)

TFF3

Human MMP Panel, Premix FCSTM07, User Mix LMPM000
MMP-1

MMP-3

MMP-8

MMP-10

MMP-13

MMP-2

MMP-7

MMP-9

MMP-12

EMMPRIN

Human Metabolic Panel, Premix FCSTM19

18

C-Peptide

Ghrelin

Glucagon

IL-6

Leptin/OB

Peptide YY

CCL2/JE/MCP-1

GLP-1

IFN-gamma

InsulinPancreatic
Polypeptide/PP

TNF-alphaB I O -T E C H N E ®
// R & D S Y S T E M S L U M I N E X P O R T F O L I O

TA B L E // 1 0

R&D Systems Luminex®
High Performance Assays Continued
Human Obesity Panel, Premix FCSTM08, User Mix LOBM000
Adiponectin/
Acrp30CCL2/MCP-1

IL-6

Leptin/OB

Serpin E1/PAI-1

C-Reactive
Protein/CRP

Comp. Factor D/
Adipsin

IL-10

Resistin

TNF-a

Human Tumor Biomarker Panel, Premix FCSTM25
AFP

CEACAM-5

Fas Ligand

IL-6

Oseopontin/OPN

Thyroglobulin

CA125

CG beta/HCGbeta

FGF basic

IL-8

Prolactin

TNF-alphaCA15-3Chromagranin A/
CHGA

Glypican-1

LeptinPSA Total

VEGF

CA19-9CYFRA21-1

HE4/WFDC2

MIF

SCF/c-kit ligand

CD40 Ligand

Fas

HGF

Neuron-specificenolase/NSE

TGF-alpha

Human XL Cytokine Panel, Premix FCSTM18B, User Mix LUXLM000B
CCL2/MCP -1

CX3CL1/
Fractalkine

GM-CSF

IL-2

IL-10

PDGF-AA

CCL3/MIP-1 alpha

CXCL1/GRO alphaGranzyme B

IL-3

IL-12 p70

PDGF-BB

CCL4/MIP-1 beta

CXCL2/GRO beta

IFN- alpha

IL-4

IL-13

TGF- alphaCCL5/RANTESCXCL10/IP-10

IFN- beta

IL-5

IL-15

TNF- alphaCCL11/Eotaxin

EGF

IFN- gamma

IL-6

IL-17A

TNF-beta

CCL19/MIP-3 beta

FGF basic/FGF-2

IL-1 alphaIL-7

IL-17E/IL-25

TRAIL

CCL20/MIP-3
alpha

Flt-3 Ligand

IL-1 beta

IL-8/CXCL8

IL-33

VEGF

CD40 Ligand

G-CSF

IL-1raIL-9

PD-L1/B7-H1

Multi-species TGF-b Panel, Premix FCSTM17, User Mix LTGM00
TGF- beta1

TGF- beta2

TGF- beta3

Mouse XL Cytokine Performance Panel, Premix FCSTM20, User Mix LMXL000
BAFF/BLyS/TNSFS13B

CXCL1/KC

I-CAM

IL-5

IL-16

LIX

CCL2/JE/MCP-1

CXCL10/IP-10/
CRG-2

IFN-gamma

IL-6

IL-17A

M-CSF

CCL3/MIP-1 alphaEGF

IL- 1 alpha/IL-1F1

IL-7

IL-18

TIMP-1

CCL4/MIP-1 beta

FGF-basic

IL-1 beta/IL-1F2

IL-9

IL-21

TNF-alphaCCL5/RANTES

Flt-3 ligand

IL-1ra/IL-1F3

IL-10

IL-27

VEGF

CCL11/Eotaxin

G-CSF

IL-2

IL-11

IL-31

CCL19/MIP-3beta

GDF-15

IL-3

IL-12 p70

LDL R

Chitinase3-like 1

GM-CSF

IL-4

IL-13

LIF

19

TA B L E // 1 0

R&D Systems Luminex®
High Performance Assays Continued
Non Human Primate XL Cytokine Panel, Premix FCSTM21
BDNF

CXCL2/GRO
beta/MIP-2/
CINC-3

GM-CSF

IL-4

IL-12 p70

PD-L1/B7-H1

CCL2/JE/MCP-1

CXCL10/IP-10/
CRG-2

Granzyme B

IL-5

IL-13

PDGF-AA

CCL5/RANTESCXCL11/I-TAC

IFN- alpha

IL-6

IL-15

PDGF-BB

CCL11/Eotaxin

CXCL13/BLC/
BCA-1

IFN- beta

IL-7

IL-17/IL-17A

TGF- alphaCCL20/MIP-3
alpha

FGF basic/FGF2/
bFGF

IFN gamma

IL-8/CXCL8

IL-21

TNF- alphaCD40 Ligand/
TNFSF5

G-CSF

IL-1 beta/IL-1F2

IL-10

MIP-1 beta

VEGF

TA B L E // 1 1

R&D Systems Luminex®
Discovery Assays
Human Discovery Assay, Catalog # LXSAHM

20

4-1BB/TNFRSF9/CD137

Angiopoietin-like Protein 4/ANGPTL4

CA125/MUC16

ADAMTS13

Angiopoietin-like Protein 6/ANGPTL6

CA15-3/MUC-1

Adiponectin/Acrp30

APP

Calbindin D

Aggrecan

APRIL/TNFSF13

Carbonic Anhydrase IX/CA9

AgRP/ART

Atrial Natriuretic Peptide/ANPCathepsin S

ALCAM/CD166

B7-H3

CCL1/I-309/TCA-3

Aldehyde Dehydrogenase 1-A1/
ALDH1A1

BAFF/BLyS/TNFSF13B

CCL11/Eotaxinalpha 1-Microglobulin

BCMA/TNFRSF17

CCL13/MCP-4

alpha 2-Macroglobulin

BDNF

CCL14/HCC-1/HCC-3

alpha-Fetoprotein/AFP

beta 2-Microglobulin

CCL15/MIP-1 deltaalpha-N-acetylglucosaminidase/
NAGLU

beta-NGF

CCL17/TARC

alpha-Synuclein

BMP-10

CCL18/PARC

Angiogenin

BMP-2

CCL19/MIP-3 beta

Angiopoietin-1

BMP-4

CCL2/JE/MCP-1

Angiopoietin-2

BMP-7

CCL20/MIP-3 alphaAngiopoietin-like Protein 3/ANGPTL3

BMP-9

CCL21/6Ckine

B I O -T E C H N E ®

R&D Systems Luminex®
Discovery Assays Continued
CCL22/MDC

Complement Factor D/Adipsin

EpCAM/TROP1

CCL23/MPIF-1

Contactin-1

EphA2

CCL24/Eotaxin-2/MPIF-2

C-Peptide

ErbB2/Her2

CCL25/TECK

C-Reactive Protein/CRP

ErbB3/Her3

CCL26/Eotaxin-3

CriptoE-Selectin/CD62E

CCL27/CTACK

CX3CL1/Fractalkine

FABP4/A-FABP

CCL28

CXCL1/GRO alpha/KC/CINC-1

Fas Ligand/TNFSF6

CCL3/MIP-1 alpha

CXCL10/IP-10/CRG-2

Fas/TNFRSF6/CD95

CCL4/MIP-1 beta

CXCL11/I-TAC

Ferritin

CCL5/RANTESCXCL13/BLC/BCA-1

Fetuin A/AHSG

CCL7/MCP-3/MARCCXCL14/BRAK

FGF acidic/FGF1

CCL8/MCP-2

CXCL16

FGF basic/FGF2/bFGF

CD117/c-kit

CXCL2/GRO beta/MIP-2/CINC-3

FGF-13

CD14

CXCL4/PF4

FGF-23CD163

CXCL5/ENA-78

Fibroblast Activation Protein alpha/FAP

CD23/Fc epsilon RII

CXCL6/GCP-2

Fibronectin

CD25/IL-2R alphaCXCL7/NAP-2

Flt-3 Ligand/FLT3L

CD27/TNFRSF7CXCL9/MIG

Follistatin-like 1/FSTL1

CD30/TNFRSF8

Cystatin C

Follistatin-related Gene Protein/FLRG

CD31/PECAM-1

DcR3/TNFRSF6B

Furin

CD40 Ligand/TNFSF5

D-dimer

Galectin-1

CD40/TNFRSF5

Dkk-1

Galectin-3

CD44

DPPIV/CD26

Galectin-3BP/MAC-2BP

CEACAM-1/CD66a

DR3/TNFRSF25

Galectin-9

CEACAM-5/CD66e

EGF

Gas6

Chemerin

EMMPRIN/CD147

G-CSF

Chitinase 3-like 1

Endocan/ESM-1

GDF-15

Coagulation Factor III/Tissue Factor

Endoglin/CD105

GDNF

Coagulation Factor XIV/Protein C

Endostatin

GITR/TNFRSF18

Collagen IV alpha 1

Endothelin-1

GM-CSF

Complement Component C2

Enolase 2/Neuron-specific Enolase

gp130

Complement Component C5a

ENPP-2/Autotaxin

Granzyme A

Complement Component C9

EN-RAGE/S100A12

Granzyme B// R & D S Y S T E M S L U M I N E X P O R T F O L I O

TA B L E // 1 1

21

TA B L E // 1 1

R&D Systems Luminex®
Discovery Assays Continued

22

Growth Hormone

IL-1ra/IL-1F3

Lymphotoxin-alpha/TNF-beta

HB-EGF

IL-2

MAdCAM-1

HE4/WFDC2

IL-21

MBL

HGF

IL-23

MCAM/CD146

HGFR/c-MET

IL-27

M-CSF

HMW Adiponectin/Acrp30

IL-28A/IFN-lambda 2

M-CSF R/CD115

HTRA2/Omi

IL-28B/IFN-lambda 3

Mesothelin

ICAM-1/CD54

IL-3

MFG-E8

IFN-alpha

IL-31

MIA

IFN-beta

IL-33

MICA

IFN-gamma

IL-34

MidkineIFN-gamma R1/CD119

IL-36 beta/IL-1F8MIF

IGFBP-1

IL-4

MMP-1

IGFBP-2

IL-4R alphaMMP-12

IGFBP-3

IL-5

MMP-13

IGFBP-4

IL-6

MMP-2

IGFBP-6

IL-6R alpha

MMP-3

IGFBP-rp1/IGFBP-7

IL-7

MMP-7

IL-1 alpha/IL-1F1

IL-8/CXCL8

MMP-8

IL-1 beta/IL-1F2

Insulin

MMP-9

IL-1 RI

ITIH4

MSP/MST1

IL-1 RII

Kallikrein 3/PSA

Myeloperoxidase/MPO

IL-10

Kallikrein 5

Myoglobin

IL-11

Kallikrein 6/Neurosin

N-Cadherin

IL-12 p70

Lactoferrin

NCAM-1/CD56

IL-12/IL-23 p40

LBP

Nectin-4

IL-13

Leptin R

Nephrin

IL-15

Leptin/OB

Neuregulin-1 beta 1/NRG1 beta 1

IL-16

LIF

Neuropilin-1

IL-17/IL-17A

LIGHT/TNFSF14

NT-3

IL-17C

Lipocalin-2/NGAL

NT-4

IL-17E/IL-25

LRG1

Oncostatin M/OSM

IL-18/IL-1F4

L-Selectin/CD62L

Osteoactivin/GPNMB

IL-19

LumicanOsteopontin/OPN

B I O -T E C H N E ®

R&D Systems Luminex®
Discovery Assays Continued
Osteoprotegerin/TNFRSF11B

SCGF/CLEC11a

TNF-alphaPark7/DJ-1

Serpin A10/ZPI

Total Inhibin

PBEF/Visfatin

Serpin A12

TRACP/PAP/ACP5

PDGF-AA

Serpin A4/Kallistatin

TRAIL R2/TNFRSF10B

PDGF-AB

Serpin A7/TBG

TRAIL R3/TNFRSF10C

PDGF-BB

Serpin B3/SCCA1TRAIL/TNFSF10

PDGF-CC

Serpin C1/Antithrombin-III

TRANCE/TNFSF11/RANK L

PDGF-DD

Serpin E1/PAI-1

TREM-1

PD-L1/B7-H1

Serpin F1/PEDF

Troponin I

Pentraxin 2/SAP

SHBG

TSLP

Pentraxin 3/TSG-14

SLPI

UCH-L1/PGP9.5

Periostin/OSF-2

SOST/Sclerostin

ULBP-1

PLA2G7/PAF-AH/Lp-PLA2SPARC

ULBP-2/5/6

PlGF

SP-D

ULBP-3

Procalcitonin

ST2/IL-33R

ULBP-4/RAET1E

Pro-Collagen I alpha 1

Stanniocalcin 1/STC-1

uPAR

Progranulin/PGRN

Syndecan-1/CD138

u-Plasminogen Activator (uPA)/Urokinase

Prolactin

Syndecan-4

Uromodulin

Properdin

TACI/TNFRSF13B

Uteroglobin/SCGB1A1

Proprotein Convertase 9/PCSK9Tau

VAP-1/AOC3

Protein S/PROS1

Tenascin C

VCAM-1/CD106

Proteinase 3/Myeloblastin/PRTN3

TFF3

VEGF

P-Selectin/CD62P

TFPI

VEGF-CRAGE/AGER

TfR (Transferrin R)

VEGFR1/Flt-1

RBP4/Retinol-Binding Protein 4

TGF-alpha

VEGFR2/KDR/Flk-1

Reg3A

Thrombomodulin/BDCA-3

VEGFR3/Flt-4

Relaxin-2

Thrombopoietin/Tpo

Vitamin D BP

Renin

Thrombospondin-2

vWF-A2

ResistinThymidine Kinase 1

ROBO4

Tie-2

S100A8

TIM-1/KIM-1/HAVCRS100A9TIMP-1

S100B

TNF RI/TNFRSF1A

SCF/c-kit Ligand

TNF RII/TNFRSF1B// R & D S Y S T E M S L U M I N E X P O R T F O L I O

TA B L E // 1 1

23

TA B L E // 1 1

R&D Systems Luminex®
Discovery Assays Continued
Mouse Discovery Assay, Catalog # LXSAMSM

24

Adiponectin/Acrp30

Endoglin/CD105

IL-7

Angiopoietin-2

FABP4/A-FABP

LDLR

BAFF/BLyS/TNFSF13B

Fas Ligand/TNFSF6

LIX

beta-NGF

FGF basic/FGF2/bFGF

M-CSF

C1qR1/CD93

FGF-21

MMP-12

CCL11/Eotaxin

G-CSF

MMP-2

CCL12/MCP-5

GDF-15

MMP-3

CCL19/MIP-3 beta

GM-CSF

MMP-8

CCL2/JE/MCP-1

Granzyme B

MMP-9

CCL20/MIP-3 alphaHaptoglobin

Nephrin

CCL21/6Ckine

HGF

Oncostatin M/OSM

CCL22/MDC

ICAM-1/CD54

Osteopontin/OPN

CCL3/MIP-1 alphaIFN-gamma

Osteoprotegerin/TNFRSF11B

CCL4/MIP-1 beta

IGFBP-1

PDGF-AA

CCL5/RANTESIGFBP-3

PDGF-BB

CCL7/MCP-3/MARC

IGF-I/IGF-1

Periostin/OSF-2

CCL8/MCP-2

IL-1 alpha/IL-1F1

PlGF-2

Chitinase 3-like 1

IL-1 beta/IL-1F2

Podocalyxin

Complement Factor D/Adipsin

IL-10

Prolactin

C-Reactive Protein/CRP

IL-12 p70

Proprotein Convertase 9/PCSK9CXCL1/GRO alpha/KC/CINC-1

IL-13

P-Selectin/CD62P

CXCL10/IP-10/CRG-2

IL-16

Renin

CXCL12/SDF-1 alphaIL-17/IL-17A

Resistin

CXCL13/BLC/BCA-1

IL-17E/IL-25

S100A8CXCL16

IL-2

S100A9CXCL2/GRO beta/MIP-2/CINC-3

IL-27

Serpin E1/PAI-1

Cystatin C

IL-3

SP-D

Dkk-1

IL-33

Syndecan-1/CD138

DPPIV/CD26

IL-4

Thrombospondin-4

EGF

IL-5

TIM-1/KIM-1/HAVCR

EGFR

IL-6

TIMP-1

EMMPRIN/CD147

IL-6R alpha

TIMP-4

B I O -T E C H N E ®

R&D Systems Luminex®
Discovery Assays Continued
TNF RI/TNFRSF1A

TRANCE/TNFSF11/RANK L

VEGFTNF RII/TNFRSF1B

TWEAK/TNFSF12VEGFR2/KDR/Flk-1

TNF-alphauPAR

Rat Discovery Assay, Catalog # LXSARMCXCL1/GRO alpha/KC/CINC-1

IL-1 alpha/IL-1F1

IL-4

CXCL2/GRO beta/MIP-2/CINC-3

IL-1 beta/IL-1F2

IL-6

CXCL3/GRO gamma/CINC-2/DCIP-1

IL-10

L-Selectin/CD62L

GM-CSF

IL-13

TIMP-1

ICAM-1/CD54

IL-18/IL-1F4

TNF-alpha

IFN-gamma

IL-2

VEGF

// R & D S Y S T E M S L U M I N E X P O R T F O L I O

TA B L E // 1 1

Porcine Discovery Assay, Catalog # LXSAPM
CD31/PECAM-1

IL-10

IL-8/CXCL8

CD34

IL-12

MMP-1

C-Reactive Protein/CRP

IL-18/IL-1F4

PDGF-BB

GM-CSF

IL-1ra/IL-1F3

Serpin E1/PAI-1

IFN-gamma

IL-2

TNF-alphaIL-1 alpha/IL-1F1

IL-4

IL-1 beta/IL-1F2

IL-6

Luminex®, Luminex® 200™, FLEXMAP 3D ®, and xMAPINTELLIFLEX® are trademarks of Luminex Corporation.

25

26

B I O -T E C H N E ®

// R & D S Y S T E M S L U M I N E X P O R T F O L I O

Notes:

27

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