Products
pluriSpin® Human PLT Depletion

19-00002-31

If you can handle
gradient centrifugation,then you can handle
pluriSpin®, too.

pluriSpin® Human PLT Depletion for processing 100 ml wholeblood.
pluriSpin® Human Monocyte Enrichment

19-01001-31

pluriSpin® Human Monocyte Depletion19-01002-31

pluriSpin® Human T Cell Enrichment

19-02001-31

pluriSpin® Human T Cell Depletion

19-02002-31

pluriSpin® Human CD4⁺ T Cell Enrichment

19-03001-31

pluriSpin® Human CD4⁺ T Cell Depletion

19-03002-31

pluriSpin® Human CD8⁺ T Cell Enrichment19-04001-31

pluriSpin® Human CD8⁺ Cell Depletion

19-04002-31

pluriSpin® Human NK Cell Enrichment

19-05001-31

pluriSpin® Human NK Cell Depletion

19-05002-31

pluriSpin® Human B Cell Enrichment

19-06001-31

pluriSpin® Human CD19⁺ Cell Depletion

19-06002-31

pluriSpin® Human Granulocyte Enrichment1

19-07001-31

pluriSpin® Human CD45 Depletion

19-08001-31

pluriSpin
Negative Cell Isolation
For Untouched Cells

All products available for processing 40 ml or 200 ml wholeblood (2 ml or 10 ml vials).
1Use Leuko Spin Medium for granulocyte enrichment.

Density Gradient Media
Leuko Spin Medium

60-00091-10

Lympho Spin Medium

60-00092-10

Lympho 24+ Spin Medium

60-00093-10

PLT Spin Medium

60-00094-10(all white blood cells)(PBMC)(PBMC from 12 - 48 hours old peripheral blood)(Platelets)

pluriSelectpluriSelect Life Science

Spring Valley, CA 91977
USADeutscher Platz 5c04103 Leipzig
Germany

Phone: 619-202-4297
support.usa@pluriselect.com

Phone: +49 341 333858-0

USApluriMate
Tubes to support centrifugation process.
pluriMate-22 ml

44-00002-10

pluriMate-15

15 ml

44-00015-10pluriMate-50

50 ml

44-00050-10

Worldwide

support@pluriselect.com

www.pluriselect.com/plurispin.html

V 1.4

✓ Affordable one step negative cell separation✓ Independent of RBC concentration

How It WorksLabelpluriSpin

Facts & Features

Add pluriSpin®
(50 µl per 1 ml sample)✓ Based on density centrifugation✓ Untouched cells for maximum viability✓ No columns, no magnets✓ No training or special equipment required✓ Isolate from whole blood, buffy coat orPurity Improvement with PLT Depletion
pluriSpin® Human PLT Depletion is designed to reduceplatelet contamination.
It increases purity of enriched cells through a density
gradient centrifugation in one step and can be combinedwith negative separation techniques such as pluriSpin®.

Unwanted cells arebound to pluriSpin
suspension

Incubate 15 minpluriSpin® Human
Monocyte Enrichment

pluriSpin® Human Monocyte
Enrichment & pluriSpin® Human
PLT Depletion

89.1% platelet contamination

20.4% platelet contamination

on pluriPlix® orrolling mixer

cord blood✓ Remove platelet contamination

Layer over density
gradient medium

with PLT Depletion

Typical Results Using pluriSpin®
Human Monocyte Enrichment
Enriched: 88.6% CD14+ cells

Start: 7% CD14+ cells in WB

Density gradient medium

Spin

15 min at 800 x g,
Room Temperature,brake oﬀ

PlasmaEnriched cells
Density gradient medium
RBCs and unwanted cells

Starting with fresh peripheral blood, the CD14+ cell
content of the enriched fraction is typically 84% - 92%.

CollectpluriMate®

PurityTarget Cell Type

Enriched Purity

Depleted Purity

Monocytes

84% - 92%

0.2% - 1%T cells

88% - 95%

0.2% - 2%

CD4+ T cells

88% - 94%

0.2% - 1%

CD8+ T cells84% - 95%

0.2% - 0.7%

NK cells

75% - 90%

0.5% - 3%

B cells

75% - 85%

0.1% - 0.4%

Desired cells
Unwanted cells
Red blood cells

Enriched cells

Wash

10‘

300 x gpluriMate® was developed for
optimal separation of leukocytes
and peripheral blood mononuclear
cells (PBMC) from whole blood andbone marrow.
Tubes are available in sizes 2 ml, 15 ml
and 50 ml, pre-filled and unfilled.