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12

http://nucleosome.kyushu-u.ac.jp

News Letter
1.
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2015
13th AprDNA:

Scientific Reports()

Scientific Reports
Nucleic Acids Research

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Scientific Reports
Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing
CENP-A and H3.3.
Arimura Y, Shirayama K, Horikoshi N, Fujita R, Taguchi H, Kagawa W, Fukagawa T, Almouzni G, Kurumizaka H
Sci. Rep. 2014 Nov 19;4:7115.
http://www.nature.com/srep/2014/141119/srep07115/full/srep07115.html

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(1) Mislocalization of the centromeric histone variant CenH3/CENP-A in human cells depends on the chaperone DAXX., Lacoste N,
Woolfe A, Tachiwana H, Garea AV, Barth T, Cantaloube S, Kurumizaka H, Imhof A, Almouzni G., Mol. Cell. 2014 Feb 20;53(4):631-644.

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Scientific Reports

H26. 11/12

Computational image analysis of colony and nuclear morphology to evaluate human induced
pluripotent stem cells
Tokunaga K, Saitoh N, Goldberg IG, Sakamoto C, Yasuda Y, Yoshida Y, Yamanaka S, Nakao M.
Sci. Rep. 2014 Nov 11;4:6996.
http://www.nature.com/srep/2014/141111/srep06996/full/srep06996.html

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Nucleic Acids Research
Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle.
Harada A, Maehara K, Sato Y, Konno D, Tachibana T, Kimura H, Ohkawa Y
Nucleic Acids Res. 2015 Jan;43(2):775-86.
http://nar.oxfordjournals.org/content/43/2/775.long

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26

原著論文
領域内共同研究による論文が 22 報発表されました。
DNA binding properties of the actin-related protein Arp8 and its role in DNA repair.
Osakabe A, Takahashi Y, Murakami H, Otawa K, Tachiwana H, Oma Y, Nishijima H, Shibahara KI, Kurumizaka H, Harata M
PLoS One. 2014 Oct 9;9(10):e108354.
Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3
Arimura Y, Shirayama K, Horikoshi N, Fujita R, Taguchi H, Kagawa W, Fukagawa T, Almouzni G and Kurumizaka H
Sci Rep, 2014, 4: 7115
Distinct features of the histone core structure in nucleosomes containing the histone H2A.B variant
Sugiyama M, Arimura Y, Shirayama K, Fujita R, Oba Y, Sato N, Inoue R, Oda T, Sato M, Heenan RK and Kurumizaka H
Biophys J, 2014, 106(10): 2206-2213
Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1
Machida S, Takaku M, Ikura M, Sun J, Suzuki H, Kobayashi W, Kinomura A, Osakabe A, Tachiwana H, Horikoshi Y, Fukuto A, Matsuda R, Ura K,
Tashiro S, Ikura T and Kurumizaka H
Sci Rep, 2014, 4: 4863
Histone H4 Lys 20 monomethylation of the CENP-A nucleosome is essential for kinetochore assembly
Hori T, Shang WH, Toyoda A, Misu S, Monma N, Ikeo K, Molina O, Vargiu G, Fujiyama A, Kimura H, Earnshaw WC and Fukagawa T
Dev Cell, 2014, 29(6): 740-749
The CENP-O complex requirement varies among different cell types
Kagawa N, Hori T, Hoki Y, Hosoya O, Tsutsui K, Saga Y, Sado T and Fukagawa T
Chromosome Res, 2014, 22(3): 293-303
Two arginine residues suppress the flexibility of nucleosomal DNA in the canonical nucleosome core
Kono H, Shirayama K, Arimura Y, Tachiwana H and Kurumizaka H
PLoS One, 2015, 10(3): e0120635
Regulation of RNA polymerase II activation by histone acetylation in single living cells
Stasevich TJ, Hayashi-Takanaka Y, Sato Y, Maehara K, Ohkawa Y, Sakata-Sogawa K, Tokunaga M, Nagase T, Nozaki N, Mcnally JG and Kimura H
Nature, 2014, 516(7530): 272-275
Evaluation of chemical fluorescent dyes as a protein conjugation partner for live cell imaging
Hayashi-Takanaka Y, Stasevich TJ, Kurumizaka H, Nozaki N and Kimura H
PLoS One, 2014, 9(9): e106271
Heterochromatin dynamics during the differentiation process revealed by the DNA methylation reporter mouse, MethylRO
Ueda J, Maehara K, Mashiko D, Ichinose T, Yao T, Hori M, Sato Y, Kimura H, Ohkawa Y and Yamagata K
Stem Cell Reports, 2014, 2(6): 910-924
Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena
Iwamoto M, Koujin T, Osakada H, Mori C, Kojidani T, Matsuda A, Asakawa H, Hiraoka Y and Haraguchi T
J Cell Sci, 2015, doi: 10.1242/jcs.167353
Fluorescence correlation spectroscopy with visible-wavelength superconducting nanowire single-photon detector
Yamashita T, Liu D, Miki S, Yamamoto J, Haraguchi T, Kinjo M, Hiraoka Y, Wang Z and Terai H
Opt Express, 2014, 22(23): 28783-28789
Externally Controllable Molecular Communication
Nakano T, Kobayashi S, Suda T, Okaie Y, Hiraoka Y, Haraguchi T
IEEE J Sel Area Comm, 2014, 32 (12), 2417-2431, doi: 10.1109/JSAC.2014.2367667

Characterization of nuclear pore complex components in fission yeast Schizosaccharomyces pombe.
Asakawa H, Yang HJ, Yamamoto TG, Ohtsuki C, Chikashige Y, Sakata-Sogawa K, Tokunaga M, Iwamoto M, Hiraoka Y, Haraguchi T
Nucleus, 5(2):149-162
Nuclear actin activates human transcription factor genes including the OCT4 gene
Yamazaki S, Yamamoto K, Tokunaga M, Sakata-Sogawa K and Harata M
Biosci Biotechnol Biochem, 2015, 79(2): 242-246
Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle
Harada A, Maehara K, Sato Y, Konno D, Tachibana T, Kimura H and Ohkawa Y
Nucleic Acids Res, 2015, 43(2): 775-786
Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1
Baba T, Otake H, Sato T, Miyabayashi K, Shishido Y, Wang CY, Shima Y, Kimura H, Yagi M, Ishihara Y, Hino S, Ogawa H, Nakao M, Yamazaki T,
Kang D, Ohkawa Y, Suyama M, Chung BC and Morohashi K
Nat Commun, 2014, 5: 3634
Meiotic nuclear movements in fission yeast are regulated by the transcription factor Mei4 downstream of a Cds1-dependent replication
checkpoint pathway
Ruan K, Yamamoto TG, Asakawa H, Chikashige Y, Masukata H, Haraguchi T and Hiraoka Y
Genes Cells, 2015, 20(3): 160-172
Chromosomes rein back the spindle pole body during horsetail movement in fission yeast meiosis
Chikashige Y, Yamane M, Okamasa K, Mori C, Fukuta N, Matsuda A, Haraguchi T and Hiraoka Y
Cell Struct Funct, 2014, 39(2): 93-100
Regulation of homologous recombinational repair by lamin B1 in radiation-induced DNA damage
Liu NA, Sun J, Kono K, Horikoshi Y, Ikura T, Tong X, Haraguchi T and Tashiro S
FASEB J, 2015, doi: 10.1096/fj.14-265546
Reorganization of damaged chromatin by the exchange of histone variant H2A.Z-2
Nishibuchi I, Suzuki H, Kinomura A, Sun J, Liu NA, Horikoshi Y, Shima H, Kusakabe M, Harata M, Fukagawa T, Ikura T, Ishida T, Nagata Y andTashiro S
Int J Radiat Oncol Biol Phys, 2014, 89(4): 736-744
Adhesion of suspension cells on a coverslip in serum-free conditions
Nakayama T, Mihara K, Kawata J, Kimura H and Saitoh H
Anal Biochem, 2014, 466(1-3)

その他 81 報の論文が発表されました。
Charge-neutralization effect of the tail regions on the histone H2A/H2B dimer structure
Saikusa K, Shimoyama S, Asano Y, Nagadoi A, Sato M, Kurumizaka H, Nishimura Y and Akashi S
Protein Sci, 2015, doi: 10.1002/pro.2673
Mass spectrometric approach for characterizing the disordered tail regions of the histone H2A/H2B dimer
Saikusa K, Nagadoi A, Hara K, Fuchigami S, Kurumizaka H, Nishimura Y and Akashi S
Anal Chem, 2015, 87(4): 2220-2227
Human tNASP promotes in vitro nucleosome assembly with histone H3.3
Kato D, Osakabe A, Tachiwana H, Tanaka H and Kurumizaka H
Biochemistry, 2015, 54(5): 1171-1179
A method for evaluating nucleosome stability with a protein-binding fluorescent dye
Taguchi H, Horikoshi N, Arimura Y and Kurumizaka H
Methods, 2014, 70(2-3): 119-126
N-terminal phosphorylation of HP1alpha increases its nucleosome-binding specificity
Nishibuchi G, Machida S, Osakabe A, Murakoshi H, Hiragami-Hamada K, Nakagawa R, Fischle W, Nishimura Y, Kurumizaka H, Tagami H andNakayama J
Nucleic Acids Res, 2014, 42(20): 12498-12511
Expression and purification of human FANCI and FANCD2 using Escherichia coli cells
Takahashi D, Sato K, Shimomuki M, Takata M and Kurumizaka H
Protein Expr Purif, 2014, 103: 8-15
FANCD2 binds CtIP and regulates DNA-end resection during DNA interstrand crosslink repair
Unno J, Itaya A, Taoka M, Sato K, Tomida J, Sakai W, Sugasawa K, Ishiai M, Ikura T, Isobe T, Kurumizaka H and Takata M
Cell Rep, 2014, 7(4): 1039-1047
Defective FANCI binding by a fanconi anemia-related FANCD2 mutant
Sato K, Ishiai M, Takata M and Kurumizaka H
PLoS One, 2014, 9(12): e114752
Structure of human nucleosome containing the testis-specific histone variant TSH2B.
Urahama T, Horikoshi N, Osakabe A, Tachiwana H, Kurumizaka H.
Acta Crystallogr F Struct Biol Commun, 2014 Apr;70(Pt 4):444-9.
Local dynamics coupled to hydration water determines DNA-sequence-dependent deformability
Nakagawa H, Yonetani Y, Nakajima K, Ohira-Kawamura S, Kikuchi T, Inamura Y, Kataoka M and Kono H
Phys Rev E Stat Nonlin Soft Matter Phys, 2014, 90(2): 022723

Intensity of Diffracted X-rays from Biomolecules with Radiation Damage Caused by Strong X-ray Pulses
Kai T, Tokuhiga A, Moribayashi K, Fukuda Y, Kono H, Go N
J. Phys. Soc. Jpn. 2014, 83: 094301 doi: 10.7566/JPSJ.83.094301
An annexin A1-FPR1 interaction contributes to necroptosis of keratinocytes in severe cutaneous adverse drug reactions
Saito N, Qiao H, Yanagi T, Shinkuma S, Nishimura K, Suto A, Fujita Y, Suzuki S, Nomura T, Nakamura H, Nagao K, Obuse C, Shimizu H and Abe R
Sci Transl Med, 2014, 6(245): 245ra295
A novel method for purification of the endogenously expressed fission yeast Set2 complex
Suzuki S, Nagao K, Obuse C, Murakami Y and Takahata S
Protein Expr Purif, 2014, 97: 44-49
CxxC-ZF domain is needed for KDM2A to demethylate histone in rDNA promoter in response to starvation
Tanaka Y, Umata T, Okamoto K, Obuse C and Tsuneoka M
Cell Struct Funct, 2014, 39(1): 79-92
Coordinated expression of H3K9 histone methyltransferases during tooth development in mice
Kamiunten T, Ideno H, Shimada A, Nakamura Y, Kimura H, Nakashima K and Nifuji A
Histochem Cell Biol, 2015, 143(3): 259-266
Phenotype Specific Analyses Reveal Distinct Regulatory Mechanism for Chronically Activated p53
Kirschner K, Samarajiwa SA, Cairns JM, Menon S, Perez-Mancera PA, Tomimatsu K, Bermejo-Rodriguez C, Ito Y, Chandra T, Narita M, Lyons SK,
Lynch AG, Kimura H, Ohbayashi T, Tavare S and Narita M
PLoS Genet, 2015, 11(3): e1005053
H3K36 Trimethylation-Mediated Epigenetic Regulation is Activated by Bam and Promotes Germ Cell Differentiation During Early
Oogenesis in Drosophila
Mukai M, Hira S, Nakamura K, Nakamura S, Kimura H, Sato M and Kobayashi S
Biol Open, 2015, 4(2): 119-124
Quantifying histone and RNA polymerase II post-translational modification dynamics in mother and daughter cells
Stasevich TJ, Sato Y, Nozaki N and Kimura H
Methods, 2014, 70(2-3): 77-88
Hierarchical molecular events driven by oocyte-specific factors lead to rapid and extensive reprogramming
Jullien J, Miyamoto K, Pasque V, Allen GE, Bradshaw CR, Garrett NJ, Halley-Stott RP, Kimura H, Ohsumi K and Gurdon JB
Mol Cell, 2014, 55(4): 524-536
Nuclear dynamics of topoisomerase IIbeta reflects its catalytic activity that is regulated by binding of RNA to the C-terminal domain
Onoda A, Hosoya O, Sano K, Kiyama K, Kimura H, Kawano S, Furuta R, Miyaji M, Tsutsui K and Tsutsui KM
Nucleic Acids Res, 2014, 42(14): 9005-9020
Nucleosomal regulation of chromatin composition and nuclear assembly revealed by histone depletion
Zierhut C, Jenness C, Kimura H and Funabiki H
Nat Struct Mol Biol, 2014, 21(7): 617-625
Loss of histone H4K20 trimethylation predicts poor prognosis in breast cancer and is associated with invasive activity
Yokoyama Y, Matsumoto A, Hieda M, Shinchi Y, Ogihara E, Hamada M, Nishioka Y, Kimura H, Yoshidome K, Tsujimoto M and Matsuura N
Breast Cancer Res, 2014, 16(3): R66
DNA methylation reader MECP2: cell type- and differentiation stage-specific protein distribution
Song C, Feodorova Y, Guy J, Peichl L, Jost KL, Kimura H, Cardoso MC, Bird A, Leonhardt H, Joffe B and Solovei I
Epigenetics Chromatin, 2014, 7: 17
Direct evidence for pitavastatin induced chromatin structure change in the KLF4 gene in endothelial cells
Maejima T, Inoue T, Kanki Y, Kohro T, Li G, Ohta Y, Kimura H, Kobayashi M, Taguchi A, Tsutsumi S, Iwanari H, Yamamoto S, Aruga H, Dong S,
Stevens JF, Poh HM, Yamamoto K, Kawamura T, Mimura I, Suehiro J, Sugiyama A, Kaneki K, Shibata H, Yoshinaka Y, Doi T, Asanuma A, Tanabe S,
Tanaka T, Minami T, Hamakubo T, Sakai J, Nozaki N, Aburatani H, Nangaku M, Ruan X, Tanabe H, Ruan Y, Ihara S, Endo A, Kodama T and Wada Y
PLoS One, 2014, 9(5): e96005
Stella preserves maternal chromosome integrity by inhibiting 5hmC-induced gammaH2AX accumulation
Nakatani T, Yamagata K, Kimura T, Oda M, Nakashima H, Hori M, Sekita Y, Arakawa T, Nakamura T and Nakano T
EMBO Rep, 2015, doi: 10.15252/embr.201439427
Improved and robust detection of cell nuclei from four dimensional fluorescence images
Bashar MK, Yamagata K and Kobayashi TJ
PLoS One, 2014, 9(7): e101891
Deficiency of a lipid droplet protein, perilipin 5, suppresses myocardial lipid accumulation, thereby preventing type 1 diabetes-induced
heart malfunction
Kuramoto K, Sakai F, Yoshinori N, Nakamura TY, Wakabayashi S, Kojidani T, Haraguchi T, Hirose F and Osumi T
Mol Cell Biol, 2014, 34(14): 2721-2731
A facile preparation of glass-supported lipid bilayers for analyzing molecular dynamics
Ito Y, Sakata-Sogawa K and Tokunaga M
Anal Sci, 2014, 30(12): 1103-1106
Proteomic identification of heterogeneous nuclear ribonucleoprotein K as a novel cold-associated autoantigen in patients with secondary
Raynaud's phenomenon
Yang L, Fujimoto M, Murota H, Serada S, Fujimoto M, Honda H, Yamada K, Suzuki K, Nishikawa A, Hosono Y, Yoneda Y, Takehara K, Imura Y,
Mimori T, Takeuchi T, Katayama I and Naka T
Rheumatology (Oxford), 2015, 54(2): 349-358

Structural basis for the selective nuclear import of the C2H2 zinc-finger protein Snail by importin beta
Choi S, Yamashita E, Yasuhara N, Song J, Son SY, Won YH, Hong HR, Shin YS, Sekimoto T, Park IY, Yoneda Y and Lee SJ
Acta Crystallogr D Biol Crystallogr, 2014, 70(Pt 4): 1050-1060
Transcriptional program of Kpna2/Importin-alpha2 regulates cellular differentiation-coupled circadian clock development in mammaliancells
Umemura Y, Koike N, Matsumoto T, Yoo SH, Chen Z, Yasuhara N, Takahashi JS and Yagita K
Proc Natl Acad Sci U S A, 2014, 111(47): E5039-5048
The linker histone in Saccharomyces cerevisiae interacts with actin-related protein 4 and both regulate chromatin structure and cellularmorphology
Georgieva M, Staneva D, Uzunova K, Efremov T, Balashev K, Harata M and Miloshev G
Int J Biochem Cell Biol, 2015, 59: 182-192
SWR1 and INO80 chromatin remodelers contribute to DNA double-strand break perinuclear anchorage site choice
Horigome C, Oma Y, Konishi T, Schmid R, Marcomini I, Hauer MH, Dion V, Harata M and Gasser SM
Mol Cell, 2014, 55(4): 626-639
Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block
Kalendova A, Kalasova I, Yamazaki S, Ulicna L, Harata M and Hozak P
Histochem Cell Biol, 2014, 142(2): 139-152
Computational image analysis of colony and nuclear morphology to evaluate human induced pluripotent stem cells
Tokunaga K, Saitoh N, Goldberg IG, Sakamoto C, Yasuda Y, Yoshida Y, Yamanaka S and Nakao M
Sci Rep, 2014, 4: 6996
Spatial re-organization of myogenic regulatory sequences temporally controls gene expression
Harada A, Mallappa C, Okada S, Butler JT, Baker SP, Lawrence JB, Ohkawa Y and Imbalzano AN
Nucleic Acids Res, 2015, 43(4): 2008-2021
MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex
Takahashi H, Takigawa I, Watanabe M, Anwar D, Shibata M, Tomomori-Sato C, Sato S, Ranjan A, Seidel CW, Tsukiyama T, Mizushima W, Hayashi M,
Ohkawa Y, Conaway JW, Conaway RC and Hatakeyama S
Nat Commun, 2015, 6: 5941
A genome-wide analysis identifies a notch-RBP-Jkappa-IL-7Ralpha axis that controls IL-17-producing gammadelta T cell homeostasis inmice
Nakamura M, Shibata K, Hatano S, Sato T, Ohkawa Y, Yamada H, Ikuta K and Yoshikai Y
J Immunol, 2015, 194(1): 243-251
Interleukin-10-producing plasmablasts exert regulatory function in autoimmune inflammation
Matsumoto M, Baba A, Yokota T, Nishikawa H, Ohkawa Y, Kayama H, Kallies A, Nutt SL, Sakaguchi S, Takeda K, Kurosaki T and Baba Y
Immunity, 2014, 41(6): 1040-1051
SraTailor: graphical user interface software for processing and visualizing ChIP-seq data
Oki S, Maehara K, Ohkawa Y and Meno C
Genes Cells, 2014, 19(12): 919-926
Genome-wide analysis of histone modifications in human endometrial stromal cells
Tamura I, Ohkawa Y, Sato T, Suyama M, Jozaki K, Okada M, Lee L, Maekawa R, Asada H, Sato S, Yamagata Y, Tamura H and Sugino N
Mol Endocrinol, 2014, 28(10): 1656-1669
Acute hyperglycemia impairs functional improvement after spinal cord injury in mice and humans
Kobayakawa K, Kumamaru H, Saiwai H, Kubota K, Ohkawa Y, Kishimoto J, Yokota K, Ideta R, Shiba K, Tozaki-Saitoh H, Inoue K, Iwamoto Y andOkada SSci Transl Med, 2014, 6(256): 256ra137
Establishment of neutralizing rat monoclonal antibodies for fibroblast growth factor-2
Tanaka M, Yamaguchi M, Shiota M, Kawamoto Y, Takahashi K, Inagaki A, Osada-Oka M, Harada A, Wanibuchi H, Izumi Y, Miura K, Iwao H and
Ohkawa Y
Monoclon Antib Immunodiagn Immunother, 2014, 33(4): 261-269
Identification of myelin transcription factor 1 (MyT1) as a subunit of the neural cell type-specific lysine-specific demethylase 1 (LSD1)complexYokoyama A, Igarashi K, Sato T, Takagi K, Otsuka IM, Shishido Y, Baba T, Ito R, Kanno J, Ohkawa Y, Morohashi K and Sugawara A
J Biol Chem, 2014, 289(26): 18152-18162
Hsc70 contributes to cancer cell survival by preventing Rab1A degradation under stress conditions
Tanaka M, Mun S, Harada A, Ohkawa Y, Inagaki A, Sano S, Takahashi K, Izumi Y, Osada-Oka M, Wanibuchi H, Yamagata M, Yukimura T, Miura K,
Shiota M and Iwao H
PLoS One, 2014, 9(5): e96785
Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment tomicrotubules
Iemura K and Tanaka K
Nat Commun, 2015, 6: 6447
CLIP-170 recruits PLK1 to kinetochores during early mitosis for chromosome alignment
Amin MA, Itoh G, Iemura K, Ikeda M and Tanaka K
J Cell Sci, 2014, 127(Pt 13): 2818-2824
Foxa2 acts as a co-activator potentiating expression of the Nurr1-induced DA phenotype via epigenetic regulation
Yi SH, He XB, Rhee YH, Park CH, Takizawa T, Nakashima K and Lee SH
Development, 2014, 141(4): 761-772

DBTMEE: a database of transcriptome in mouse early embryos
Park SJ, Shirahige K, Ohsugi M and Nakai K
Nucleic Acids Res, 2015, 43(Database issue): D771-776
The 19S proteasome subunit Rpt3 regulates distribution of CENP-A by associating with centromeric chromatin
Kitagawa T, Ishii K, Takeda K and Matsumoto T
Nat Commun, 2014, 5: 3597
The International Nucleome Consortium
Tashiro S and Lanctot C
Nucleus, 2015, 1-4, doi: 10.1080/19491034.2015.1022703
The transcription repressors Bach2 and Bach1 promote B cell development by repressing the myeloid program
Itoh-Nakadai A, Hikota R, Muto A, Kometani K, Watanabe-Matsui M, Sato Y, Kobayashi M, Nakamura A, Miura Y, Yano Y, Tashiro S, Sun J, Ikawa T,
Ochiai K, Kurosaki T and Igarashi K
Nat Immunol, 2014, 15(12): 1171-1180
Smoking cessation reverses DNA double-strand breaks in human mononuclear cells
Ishida M, Ishida T, Tashiro S, Uchida H, Sakai C, Hironobe N, Miura K, Hashimoto Y, Arihiro K, Chayama K, Kihara Y and Yoshizumi M
PLoS One, 2014, 9(8): e103993
X chromosome reactivation dynamics reveal stages of reprogramming to pluripotency
Pasque V, Tchieu J, Karnik R, Uyeda M, Sadhu Dimashkie A, Case D, Papp B, Bonora G, Patel S, Ho R, Schmidt R, Mckee R, Sado T, Tada T,
Meissner A and Plath K
Cell, 2014, 159(7): 1681-1697
Amplified and selective assay of collagens by enzymatic and fluorescent reactions
Yasmin H, Kabashima T, Rahman MS, Shibata T and Kai M
Sci Rep, 2014, 4: 4950
Carbon nanofiber-based luminol-biotin probe for sensitive chemiluminescence detection of protein
Baj S, Krawczyk T, Pradel N, Azam MG, Shibata T, Dragusha S, Skutil K, Pawlyta M and Kai M
Anal Sci, 2014, 30(11): 1051-1056
Effect of Atmospheric-Pressure Helium Plasma Jet on Cell Culture Medium
Takamura N, Wang D, Satoh T, Namihira T, Saitoh H, Akiyama H
ELECTRON COMM JPN, 2014, 97(11): 65-73, doi: 10.1002/ecj.11636
SUMO-modification and elimination of the active DNA demethylation enzyme TDG in cultured human cells
Moriyama T, Fujimitsu Y, Yoshikai Y, Sasano T, Yamada K, Murakami M, Urano T, Sugasawa K and Saitoh H
Biochem Biophys Res Commun, 2014, 447(3): 419-424
The SUMO-targeted ubiquitin ligase RNF4 localizes to etoposide-exposed mitotic chromosomes: implication for a novel DNA damage
response during mitosis
Saito M, Fujimitsu Y, Sasano T, Yoshikai Y, Ban-Ishihara R, Nariai Y, Urano T and Saitoh H
Biochem Biophys Res Commun, 2014, 447(1): 83-88
Inhibition of protein SUMOylation by davidiin, an ellagitannin from Davidia involucrata
Takemoto M, Kawamura Y, Hirohama M, Yamaguchi Y, Handa H, Saitoh H, Nakao Y, Kawada M, Khalid K, Koshino H, Kimura K, Ito A and Yoshida M
J Antibiot (Tokyo), 2014, 67(4): 335-338
Assay methods for small ubiquitin-like modifier (SUMO)-SUMO-interacting motif (SIM) interactions in vivo and in vitro using a
split-luciferase complementation system
Hirohama M, Voet AR, Ozawa T, Saitoh H, Nakao Y, Zhang KY, Ito A and Yoshida M
Anal Biochem, 2014, 448(92-94
Construction of a mouse Aos1-Uba2 chimeric SUMO-E1 enzyme, mAU, and its expression in baculovirus-insect cells
Nakayama T, Yuasa E, Kanemaru A, Saito M and Saitoh H
Bioengineered, 2014, 5(2): 133-137
Genomic DNAs in a human leukemia cell line unfold after cold shock, with formation of neutrophil extracellular trap-like structures
Kawata J, Kikuchi M and Saitoh H
Biotechnol Lett, 2014, 36(2): 241-250
Antagonistic controls of chromatin and mrna start site selection by Tup family corepressors and the CCAAT-binding factor
Asada R, Takemata N, Hoffman CS, Ohta K and Hirota K
Mol Cell Biol, 2015, 35(5): 847-855
The POLD3 subunit of DNA polymerase delta can promote translesion synthesis independently of DNA polymerase zeta
Hirota K, Yoshikiyo K, Guilbaud G, Tsurimoto T, Murai J, Tsuda M, Phillips LG, Narita T, Nishihara K, Kobayashi K, Yamada K, Nakamura J, Pommier
Y, Lehmann A, Sale JE and Takeda S
Nucleic Acids Res, 2015, 43(3): 1671-1683
Development of a Targeted Flip-in System in Avian DT40 Cells
Kobayashi K, Fujii T, Asada R, Ooka M and Hirota K
PLoS One, 2015, 10(3): e0122006
Rad18 and Rnf8 facilitate homologous recombination by two distinct mechanisms, promoting Rad51 focus formation and suppressing thetoxic effect of nonhomologous end joining
Kobayashi S, Kasaishi Y, Nakada S, Takagi T, Era S, Motegi A, Chiu RK, Takeda S and Hirota K
Oncogene, 2014, doi: 10.1038/onc.2014.371
SUMO-targeted ubiquitin ligase RNF4 plays a critical role in preventing chromosome loss
Hirota K, Tsuda M, Murai J, Takagi T, Keka IS, Narita T, Fujita M, Sasanuma H, Kobayashi J and Takeda S
Genes Cells, 2014, 19(10): 743-754

RNA cytidine acetyltransferase of small-subunit ribosomal RNA: identification of acetylation sites and the responsible acetyltransferase infission yeast, Schizosaccharomyces pombe
Taoka M, Ishikawa D, Nobe Y, Ishikawa H, Yamauchi Y, Terukina G, Nakayama H, Hirota K, Takahashi N and Isobe T
PLoS One, 2014, 9(11): e112156
RNase MRP cleaves pre-tRNASer-Met in the tRNA maturation pathway
Saito Y, Takeda J, Adachi K, Nobe Y, Kobayashi J, Hirota K, Oliveira DV, Taoka M and Isobe T
PLoS One, 2014, 9(11): e112488
Evolution of pre-existing versus acquired resistance to platinum drugs and PARP inhibitors in BRCA-associated cancers
Yamamoto KN, Hirota K, Takeda S and Haeno H
PLoS One, 2014, 9(8): e105724
Molecular basis for SMC rod formation and its dissolution upon DNA binding
Soh YM, Burmann F, Shin HC, Oda T, Jin KS, Toseland CP, Kim C, Lee H, Kim SJ, Kong MS, Durand-Diebold ML, Kim YG, Kim HM, Lee NK, Sato M,
Oh BH and Gruber S
Mol Cell, 2015, 57(2): 290-303
Fidelity consequences of the impaired interaction between DNA polymerase epsilon and the GINS complex
Garbacz M, Araki H, Flis K, Bebenek A, Zawada AE, Jonczyk P, Makiela-Dzbenska K and Fijalkowska IJ
DNA Repair (Amst), 2015, doi: 10.1016/j.dnarep.2015.02.007
Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin
Itou H, Muramatsu S, Shirakihara Y and Araki H
Structure, 2014, 22(9): 1341-1347
Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells
Gallego-Paez LM, Tanaka H, Bando M, Takahashi M, Nozaki N, Nakato R, Shirahige K and Hirota T
Mol Biol Cell, 2014, 25(2): 302-317
Structural and functional analysis of Hikeshi, a new nuclear transport receptor of Hsp70s
Song J, Kose S, Watanabe A, Son SY, Choi S, Hong H, Yamashita E, Park IY, Imamoto N and Lee SJ
Acta Crystallogr D Biol Crystallogr, 2015, 71(Pt 3): 473-483
Analytic 3D imaging of mammalian nucleus at nanoscale using coherent x-rays and optical fluorescence microscopy
Song C, Takagi M, Park J, Xu R, Gallagher-Jones M, Imamoto N and Ishikawa T
Biophys J, 2014, 107(5): 1074-1081
Ki67 antigen contributes to the timely accumulation of protein phosphatase 1gamma on anaphase chromosomes
Takagi M, Nishiyama Y, Taguchi A and Imamoto N
J Biol Chem, 2014, 289(33): 22877-22887
Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery
Booth DG, Takagi M, Sanchez-Pulido L, Petfalski E, Vargiu G, Samejima K, Imamoto N, Ponting CP, Tollervey D, Earnshaw WC and Vagnarelli P
Elife, 2014, 3, e01641
The Schizosaccharomyces pombe Hikeshi/Opi10 protein has similar biochemical functions to its human homolog but acts in different
physiological contexts
Oda Y, Kimura M, Kose S, Fasken MB, Corbett AH and Imamoto N
FEBS Lett, 2014, 588(10): 1899-1905
A novel facility for 3D micro-irradiation of living cells in a controlled environment by MeV ions
Mackel V, Meissl W, Ikeda T, Clever M, Meissl E, Kobayashi T, Kojima TM, Imamoto N, Ogiwara K and Yamazaki Y
Rev Sci Instrum, 2014, 85(1): 014302

英文総説
領域内共同研究による英文総説が 1 報発表されました。
Visualization of epigenetic modifications in preimplantation embryos
Kimura H and Yamagata K
Methods Mol Biol, 2015, 1222: 127-147

その他 11 報の英文総説が発表されました。
Nucleosome organization and chromatin dynamics in telomeres
Ichikawa Y, Nishimura Y, Kurumizaka H and Shimizu M
Biomol Concepts, 2015, 6(1): 67-75
Histone modification sensors in living cells
Kimura H and Sato Y
Optical Probes in Biology (Jin Zhang, Sohum Mehta, and Carsten Schultz ed), CRC Press, 2015
Histone modifications associated with cancer cell migration and invasion
Hieda M, Matsuura N and Kimura H
Methods Mol Biol, 2015, 1238: 301-317
Live CLEM imaging to analyze nuclear structures at high resolution
Haraguchi T, Osakada H and Koujin T
Methods Mol Biol, 2015, 1262: 89-103

Current view of the potential roles of proteins enriched on the inactive X chromosome
Nakajima T and Sado T
Genes Genet Syst, 2014, 89(4): 151-157
Reconstitution of nucleocytoplasmic transport using digitonin-permeabilized cells
Kose S, Funakoshi T and Imamoto N
Methods Mol Biol, 2015, 1262: 291-303
Novel approaches for the identification of nuclear transport receptor substrates
Kimura M, Thakar K, Karaca S, Imamoto N and Kehlenbach RH
Methods Cell Biol, 2014, 122: 353-378
Analysis of nucleocytoplasmic transport in digitonin-permeabilized cells under different cellular conditions
Furuta M, Kose S, Kehlenbach RH and Imamoto N
Methods Cell Biol, 2014, 122: 331-352
Cell-fusion method to visualize interphase nuclear pore formation
Maeshima K, Funakoshi T and Imamoto N
Methods Cell Biol, 2014, 122: 239-254
Nucleocytoplasmic transport under stress conditions and its role in HSP70 chaperone systems
Kose S and Imamoto N
Biochim Biophys Acta, 2014, 1840(9): 2953-2960
Biological significance of the importin-beta family-dependent nucleocytoplasmic transport pathways
Kimura M and Imamoto N
Traffic, 2014, 15(7): 727-748
Control of nuclear size by NPC proteinsTakagi M and Imamoto N
Adv Exp Med Biol, 2014, 773: 571-591

和文総説・著書（分担執筆）
22 報

特許
4 件（出願中を含む）

アウトリーチ活動
43 件

受賞
2014

7

8

2014

11

1

2015

3

14

15

5.

Chromatin Structure, Dynamics, and Function
http://www.knt-ec.net/2015/iscsdf/
2015

8

23-26

Invited speakers:
Frederic Berger (GMI, Austria)
Kerstin Bystricky (Univ. Toulouse, France)
Philippe Collas (Univ. Oslo, Norway)
Jessica Downs (University of Sussex, UK)
Roland Foisner (MFPL, Austria)
Peter Fraser (Babraham Inst., UK)
Martin W. Hetzer (Salk Inst., USA)
Anthony Imbalzano (Univ. Mass, USA)
Joachim Lingner (EPFL, Switzerland)
Michael Guy Poirier (Ohio State Univ., USA)
Kannanganattu V. Prasanth (Univ. Illinois, USA)Maria-Elena Torres-Padilla (IGBMC, France)
Paul A. Wade (NIEHS, NIH, USA

2015

5

7-9

編集後記：本号は年度末に発行する予定でしたが、諸事に忙殺されてかないませんでした。書類を早目に片づけられる能力が欲しいです。昨年度はたくさんの成果が得られ、皆様のご活躍がうかがわれます。また、領域内共同研究による成果も多く発表されています。
今年度は中間評価もありますので、引き続頑張っていきましょう。

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