MiSeq®

Run PCR with a
LymphoTrack Dx
Assay

Purify amplicons

Quantify& prepare
libraryPrepare
DNAsamples

Unparalleledworkflow simplicity
LymphoTrack Dx Assays put lg/ TCR
next-generation sequencing at your fingertips.
Load & run
MiSeq®

Analyze

Process datawith included
bioinformatics
software

These are in vitro diagnostic products, and are not available for sale or use within North America.

www.invivoscribe.com

Invivoscribe
Tel +1 858.224.6600 | Fax +1 858.224.6601
sales@invivoscribe.com
10222 Barnes Canyon Rd., Bldg. 1San Diego, CA 92121 | USA

Invivoscribe Technologies, SARL
Tel +33 (0)4.42.01.78.10 | Fax +33 (0)4.88.56.22.89
sales-eu@invivoscribe.com
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ZI Athélia IV | 13600 La Ciotat | FranceMiSeq®

Workflow Summary	Allow the Master Mix to thaw;
01
then gently vortex to mix.

07	In a containment hood or dead air box, pipette 45 µL
02
of Master Mix into individual wells of a PCR plate(use a different indexed Master Mix for each sampleand control).

03	Add 5 µL of DNA (at a minimum concentration of0410 ng/µL) from the unknown samples and 5µLs of	Add 0.2 µL EagleTaq™ DNA polymerase to eachMaster Mix.	Add 5 µL of molecular biology grade water to the05
well containing the Master Mix for the no templatecontrol then pipette up and down 5-10 times to mix.	Seal the plate and amplify target DNA using the06
following thermal cycler program:Temperature108	Quantify amplicons using the KAPA™ libraryquantification kit according to the kit instructions.
Dilute amplicons 1:4,000 before proceeding to qPCR.	Pool equal amounts of amplicons from samples and09
positive and negative controls (do not include the nocontrols to wells containing the respective Master Mix
reactions then pipette up and down 5-10 times to mix.

Step	Purify the PCR products using the AgencourtR AMPureR
XP PCR Purification system. Add *50 µL of particles toeach 50 µL reaction; elute DNA in 25 µL of eluant. *For theLymphoTrack TRB Assay: Add 35 µL of particles & proceedas otherwise directed.

TimeCycle

95 oC

7 minutes

1

295 oC

45 seconds

360 oC45 seconds

4o72 C

90 seconds

572 oC615 oC

10 minutes∞29x*
1template control), dilute 1:1,000 then quantify the libraryusing the KAPA library quantification kit.

10	oad 600 µL of denatured and diluted library on the11 LMiSeq
Reagent Cartridge.	Denature and dilute the library.

12	
Set up a MiSeq sample sheet using the Illumina®
Experiment Manager or provided Sample Sheet *.csv
file.

13	Analyze the acquired data using the LymphoTrack Dx
14 Software for the MiSeq.	Start the MiSeq run.

1*LymphoTrack IGHV Leader Somatic Hypermutation Assay (32 cycles)

CATALOG #9-121-0129

PRODUCTS
LymphoTrack® Dx IGH FR1/2/3 Assay Kit A - MiSeq®

QUANTITY
8 + 8 + 8 indices (5 sequencing reactions each)

9-121-0139

LymphoTrack® Dx IGH FR1/2/3 Assay Panel - MiSeq®

24 + 24 + 24 indices (5 sequencing reactions each)

9-121-0009

LymphoTrack Dx IGH FR1 Assay Kit A - MiSeq
LymphoTrack® Dx IGH FR1 Assay Panel - MiSeq®

8 indices (5 sequencing reactions each)

9-121-0039
9-121-0089

LymphoTrack® Dx IGH FR2 Assay Kit A - MiSeq®

8 indices (5 sequencing reactions each)

9-121-0099

LymphoTrack® Dx IGH FR2 Assay Panel - MiSeq®

24 indices (5 sequencing reactions each)

9-121-0109

LymphoTrack® Dx IGH FR3 Assay Kit A - MiSeq®

8 indices (5 sequencing reactions each)

9-121-0119

LymphoTrack® Dx IGH FR3 Assay Panel - MiSeq®

24 indices (5 sequencing reactions each)

9-121-0059
9-121-0069

LymphoTrack® Dx IGHV Leader Somatic Hypermutation Assay Kit A - MiSeq®
LymphoTrack® Dx IGHV Leader Somatic Hypermutation Assay Panel - MiSeq®

8 indices (5 sequencing reactions each)
24 indices (5 sequencing reactions each)

9-122-0009

LymphoTrack® Dx IGK Assay Kit A - MiSeq®

8 indices (5 sequencing reactions each)

9-122-0019

LymphoTrack® Dx IGK Assay Panel - MiSeq®

24 indices (5 sequencing reactions each)

9-225-0009

LymphoTrack® Dx TRB Assay Kit A - MiSeq®

8 indices (5 sequencing reactions each)

9-225-0019

LymphoTrack® Dx TRB Assay Panel - MiSeq®

24 indices (5 sequencing reactions each)

9-227-0019

LymphoTrack Dx TRG Assay Kit A - MiSeq

8 indices (5 sequencing reactions each)

9-227-0009

LymphoTrack® Dx TRG Assay Panel - MiSeq®
LymphoTrack® Dx Software - MiSeq®

9-500-0009

®

®

®

®

24 indices (5 sequencing reactions each)

24 indices (5 sequencing reactions each)
1 CD complimentary with purchase

Storage Conditions: -85 °C to 65 °C (DNA controls may be separated from kits and stored at 2 °C to 8 °C).
These are in vitro diagnostic products, and are not available for sale or use within North America.
©2021 Invivoscribe, Inc. (Invivoscribe®). All rights reserved. The trademarks mentioned herein are the property of Invivoscribe and/or its affiliates,or (as to the trademarks of others used herein) their respective owners. ILLUMINA® and Miseq® are registered trademarks of Illumina, Inc. AGENCOURT® and AMPURE®
are registered trademarks of Beckman Coulter, Inc. EAGLETAQ™ is a trademark of Roche. KAPA™ is a trademark of Kapa Biosystems.

M-0046 Rev01 | May 2021