pluriBead®

FAST, EASY, GENTLE!

Application Example

Clinical studies

pluriSelectpluriSelectSpring Valley, CA 91977
USADeutscher Platz 5c04103 Leipzig
Germany

Phone: 619-928-9265
support.usa@pluriselect.com
sales.usa@pluriselect.com

Phone: +49 341 333858-0

USA

www.pluriselect.com

Worldwide

support@pluriselect.com
sales@pluriselect.com

V 1.1

Clinical studies with pluriBead®

pluriBead® Cascade
Simultaneous isolation for two different targets

Introduction
pluriBead® allows for positive non-magnetic cell isolation fromany sample material. The procedure is simple: Sieve thepluriBead® with bound target cells down a strainer; pluriBead®
with your target cells stays on top, while the unwanted cells runthrough. After detaching, target cells are ready.

pluriBead® and clinical studies
When performing clinical studies or trials, sample material fromyour patients is rare. The ability to separate more than one cell
type from the same complex sample enables a new variety ofanalysis for studies in clinical trials and clinical research.
Current methods for blood analysis are serviceable for immediate stabilization of the blood (PAXGene®), but analysis of single
cell populations (magnetic cell sorting) is time and manpower
consuming. A lot of important sample material is wasted.

pluriBead® Cascade allows for the isolation of two cell types atthe same time. Just combine S-pluriBead® and M-pluriBead®.
Due to their different bead sizes, it is possible to incubate bothat the same time and separate afterwards through different
mesh sizes.
You need an S-pluriBead® Kit and a compatible M-pluriBead® Kit.

Cell separation scheme:

Wash
BufferLabeling

Incubation

IsolationApplication examples:
CD4+, CD8+ (helper T cell, cytotoxic T cell)
CD3+, CD19+ (T cell, B cell)CD14+, CD15+ (monocyte, granulocyte)

Current methods

pluriBead®

Limitation of possible targets(no proteins, viruses ormicroorganisms)

All types of targets, including
proteins, viruses andmicroorganisms

pluriBead® Step by Step

Cost-intensive equipment, timeconsuming procedure, highnumber of laboratory staff

No extra tools (except a mixer
and a centrifuge), fast procedure,
minimal staff requirement

Very fast, but only for global
analysis

Simple cell isolation within 10 30 minutes

Cells cannot be sorted afterstabilization

Flow-through can be used forfurther isolation steps

Small sample volume (2 ml wholeblood per tube)

Works with samples from
0.2 - 45 ml

Magnetic cell separation
Only for one cell population
No separation of different cell
types

Different targets from one
sample via differently sizedcatcher particles and strainers

Sample preparation needed

Direct application in whole bloodand other biological sera

Key feature: Multi-target cell isolation with pluriBead®
Find both methods on the right.

Processing**After isolation and washing, both target cell types can be used for stabilization, lysis or detachment.

Their shortcomings are numerous, but pluriBead® helps to overcome these deficits.

PAXGene® and Tempus™ BloodRNA

Washing

Sequential isolation for up to six different targets
pluriBead® Step by Step involves chain cell isolation withpluriBead® kits of various antibodies. This allows for theisolation of up to six different cell types from the same
sample.
Cell separation scheme:

Detachment
bufferWash
BufferLabelingIncubation

Isolation

Washing

Detachment

Labeling

Incubation

Isolation

After each sieving step (isolation),
you can already start to label theremaining sample for isolating a
different cell type

Application examples:
CD3+, CD14+, CD15+, CD19+ (T cell, monocyte, granulocyte, B cell)
CD3+, CD9+, CD15+, CD235a+ (T cell, platelet, granulocyte, RBC)
CD4+, CD8+, CD19+ (helper T cell, cytotoxic T cell, B cell)