ONCOLOGY

Colon Cancer
Mutation
Profiling

Conquer Sample Limitations
In Colon Cancer Testing
Reliable detection of low abundance somatic mutations in colorectal cancer samples canbe challenging due to the small specimen size and low percentage of tumor cells. New
testing guidelines recommend expanded RAS and RAF genotyping, further constraining thelimited specimens due to the increased number of targets.

AGENA'S SOLUTION
UltraSEEK™ Colon Panel
Enables study of disease progression and resistance from CTCs and ctDNA, detecting over
100 variants from a single blood draw at as low as 0.1% minor allele frequency (MAF).1iPLEX® HS Colon Panel
Facilitates variant detection across 86 variants, as low as 1% MAF from poor quality anddegraded samples such as FFPE tissue and cytology blocks.2

For Research Use Only.
Not for use in diagnostic procedures.

Genes & Mutations for Colon Panels
Pre-designed panels for the identification of prognostic and therapeutic markers across 5 genes
for comprehensive profiling of colon cancer.
GeneCoverage*# of Variants
UltraSEEK Colon Panel

iPLEX HS Colon Panel

53BRAFCodons 469 (exon 11), 594, 600 (exon 15)

EGFR

Extracellular domain mutations in exon 12

102

KRAS

Codons 12, 13 (exon 2), 59, 61 (exo 3), 117 and 146 (exon 4)

48

47

NRAS

Codons 12, 13 (exon 2), 59, 61 (exon 3), 117 and 146 (exon 4)

40

30

PIK3CA

Codons 542, 545 (exon 9) and 1047 (exon 20)

44

107

86

Total Variants* Complete variant list available upon request

ASSAY WORKFLOW
DNA to data in as little as 8 hours with minimal manual processing time enables greater lab efficiency.
Simplified reporting with automated software generates clear results.

ORDERING INFORMATION
Catalog No.

ItemSample Type# Samples

Chip Format

13142F

Complete UltraSEEK Colon Panel Set

13144F

Complete UltraSEEK Colon Panel Set

Plasma3296

Plasma809613150

Complete UltraSEEK Colon Panel Set

Plasma

32384

13151

Complete UltraSEEK Colon Panel Set

Plasma80384

13130F

Complete iPLEX HS Colon Panel Set

Tissue48

9613132F

Complete iPLEX HS Colon Panel Set

Tissue

120

96

13134

Complete iPLEX HS Colon Panel Set

Tissue

48384

13135

Complete iPLEX HS Colon Panel Set

Tissue120384The panel sets contain assay specific primers and all the required reagents to process DNA samples on the MassARRAY system.

References
1. R. Avula et al. Assessment of UltraSEEK Colon Cancer Panel for Detection of Low Frequency Somatic Mutations in Blood. Poster session presented at:
Association of Molecular Pathology Annual Meeting; 2017 Nov 16-18; Salt Lake City, UT.
2. R.T. Birse, D. Irwin. Reliable Detection of Low Abundance Somatic Mutations of EGFR, KRAS, BRAF, NRAS and PIK3CA in Metastatic Colorectal
Adenocarcinomas Using iPLEX HS, a New Highly Sensitive Assay for MassARRAY. Poster session presented at Association of Molecular Pathology Annual
Meeting; 2016 Nov 10-12; Charlotte, NC.

For Research Use Only. Not for use in diagnostic procedures.

Agena Bioscience, Inc.
4755 Eastgate MallSan Diego, CA 92121
Phone: +1.858.882.2800

ONC0006 01

Order Desk: +1.858.202.9301
Order Desk Fax: +1.858.202.9220
orderdesk@agenabio.com
Web: agenabio.com

USEUAP
CN+1.877.4.GENOME+49.40.899676.0+61.7.3088.1600+86.21.6427.0566

MassARRAY, iPLEX and Agena Bioscience are registered trademarks of Agena Bioscience, Inc.
UltraSEEK is a trademark of Agena Bioscience, Inc. ©2018 Agena Bioscience, Inc. All rights reserved.