Universal Mixer

MAN UA L

pluriPlix®

02

Introduction

fig. pluriPlix®

pluriPlix® Content
NumberComponents

1

1-armed pluriPlix® or2-armed pluriPlix®

1 or 2

Sample holder - withmagnetic connectors

1

ManualImportant Notes to Read before Use

Attention: Magnetism - Magnetic field: Notethat the mixer and the sample holder containpowerful magnets. Avoid direct contact withmagnetic media. Malfunction or failure ofcardiac pacemakers may be caused throughstrong magnetic fields. Disturbance of „nonanti-magnetic“ clocks and other mechanicaldevices. Disruption and loss of media possible. Threat to electronic or other devices:
The magnetic field of the magnets is verystrong and far reaching. Among the mostvulnerable devices are TVs and monitors,
credit cards, computer diskettes and otherstorage devices, video tapes, hearing aids
and heart pacemakers, etc. pluriPlix® works
in conjunction with a magnetic stirrer only.
When used on a magnetic stirrer, the heating
unit of the magnetic stirrer must be turned
off and cooled off.

Introduction
Product Information• This product contains no electricalcomponents.
• Do not open the device. Components can
not be reassembled.

Loading Capacity

Additional materials required whenusing pluriPlix® with pluriBead®

pluriPlix® is a non-electric universal mixer.
It can be used for the incubation of fluid
samples.

pluriPlix® is designed for a maximum loadof 4 x 50 ml reaction tubes per sample
holder. The maximum weight of 240 g
per holder should not be exceeded. For
maximum load operation we recommend amagnetic stirrrer with 10 watt power output.
The driving energy is low, so overloading
should be avoided. There is no hazard to theuser through the input power.

Blocking the Rotation of pluriPlix®

Do not block the rotation movement of thepluriPlix®. Mind the correct adjustment ofthe reaction tubes in the sample holder.

Storage & Stability

pluriPlix® should be stored and operated
in a dry environment (humidity 0-70%).
pluriPlix® works at a temperature rangeof 0°C to 45°C. Deviant usage will result
in reduced performance.

Safety Information

To avoid the risk of infection (e.g. from HIVor hepatitis B viruses) or injury when working with biological and chemical materials,
always wear a suitable lab coat, disposable
gloves and protective goggles.

Warnings & Limitations• When using pluriPlix® on a magnetic stirrer
the heating unit must be turned off andcooled off.
• This product is developed for laboratory
use only. Users must follow theappropriate laboratory guidelines.

03• Disposable gloves• Pipettes• Filter tips or Pipette tips
• Sample tubes

Maintenance & Cleaning

pluriPlix® operates maintenance free.
Clean the base unit with cleaning tissue orwith 70% alcohol. The mixer or any components should not be submerged in liquids
or cleaned in a dish washer. Autoclaving willlead to the destruction of the mixer and thetube holder.

04

Handling

Step 01 place pluriPlix® onmagnetic stirrer

1 Attach the sample holder to pluriPlix®
			 in vertical position.
2 Attach the sample holder to pluriPlix®
						 in horizontal position.
3 Attach the sample holder to pluriPlix®
						 in the favored angle.

• 0 degree angle: Bring the shortest markon the sample holder fitting in alignment
with the nose of the axle.

Place the pluriPlix® mixer on a magnetic stirrer. Make sure that the heating unit of yourmagnetic stirrer is turned off and cooled off.
Position the mixer in the center of the magnetic stirrer so that the magnetic force of thestirrer is optimally transferred to the pluriPlix® drive shaft.

Step 02 attach sample
holder in favored angle
for overhead or tilting
rotation mixing

1• 10 degree angle: Bring the longest markon the sample holder fitting in alignment
with the nose of the axle.
• 2.5, 5 or 7.5 degree angle:
Use the intervals to attach the sample
holder to pluriPlix®

Step 03 load pluriPlix®
with samples and adjust
tubes

4567

2

10 degrees
4 The pluriBead® mixing container 					 should be positioned in the sample			 holder so that it fits tightly.

35 Lids of larger reaction tubes						 (e.g. 50 ml tubes) should face the			 pluriPlix® housing. Adjust the lids close			 to the sample holder to avoid blocking			 of the rotation.

Handling
6 For overhead mixing, tubes should be			 centered in the sample holder.

05

Step 06 switch off
magnetic stirrer			
7 Differently sized slots in the sample			 holder also allow for the mixing			 of small reaction tubes			 (e.g. 1 - 2 ml tubes).
Load your samples onto the pluriPlix®
sample holder. Make sure that the lids areproperly closed to prevent leakage of sample material. Adjust the reaction tubes so as
to allow the sample holder to rotate freely
without being blocked. Tubes may be
adjusted so that the screw cap of thereaction tube faces the pluriPlix® housing.

Step 04 adjust rotation
speed

Slowly increase the speed of your magnetic
stirrer to adjust the rotation speed.

Step 05 mix samples

Rotate / mix your samples for the designated period of time.

After completing the mixing procedure, switch
off the magnetic stirrer, remove the sample
holder from pluriPlix® and remove the reaction tubes from the sample holder.

06

Using pluriPlix® in connection with pluriBead®

Using pluriPlix® in connection with pluriBead® cell separation systems ensures thoroughand gentle mixing of all samples as well as very low cell stress. For the use of pluriBead®
cell isolation systems please refer to the pluriBead® manual.

10 degrees• add pluriBead®
• add sample• close lids

Add the appropriate amount of pluriBead®
to the mixing container. Add your sample
material and close the lids of the mixing
container tightly. For more detailed information refer to pluriBead® manual.

• place pluriPlix®
on magnetic
stirrer• load pluriPlix®
with samples

Place the pluriPlix® mixer on a magnetic
stirrer. Make sure to turn off the heating unitof your magnetic stirrer. Load your samples
onto the pluriPlix® sample holder. Make surethat the lids are properly closed to prevent
leakage.

• attach sample
holder in 10
degree angle

Attach the sample holder to pluriPlix® in a
10 degree angle for thorough and gentle
mixing of the sample. For that purpose,bring the longest mark on the sample
holder fitting in alignment with the nose ofthe axle.

• adjust tubes

Larger 50 ml reaction tubes should beadjusted so that the screw cap faces thepluriPlix housing.

• adjust rotation
speed• mix samples• switch off
magnetic
stirrer

Slowly increase the speed of your magnetic
stirrer and mix your samples. Afterwards,
switch off the magnetic stirrer, remove thereaction tube holder from pluriPlix® andremove the tubes from the tube holder.
Afterwards, switch off the magnetic stirrer,
remove the sample holder from pluriPlixand unload the tubes from the sampleholder.

Questions & Anwsers

Question

Supporting Note

SolutionWhy does mypluriPlix not mix ?

• pluriPlix® drive shaft
is not in contact with themagnetic field of the magnetic stirrer• place pluriPlix in the centerof magnetic stirrer• speed of magnetic stirrer
is increased too fast

• switch off speed of themagnetic stirrer and thenslowly increase it again• pluriPlix® is overloaded• do not exceed the total
weight of 240 g per sample
holder and balance out thetubes evenly• samples on pluriPlix® are
portioned unequally• arrange the samples sothat the weight on every
sample holder is nearly equal• tubes are blocking the rotation of the sample holder• adjust the tubes so thatthey do not obstruct the rotation of the sample holder• the rotation speed of thesamples can be adjustedwith the speed dial of themagnetic stirrer.

• Method 1 (magnetic stirrer
has no RPM display): place
pluriPlix® onto the magneticstirrer. Locate the nose on
the axle and set the speed
dial of the mixer to a specificspeed. Count the revolutions
per 1 minute. If the count is
between 10 and 15, speed
is appropriate for use withpluriBead® separation kit.

How do I calculate therotation speed of mysamples ?

• For the use with the pluriBead® cell separation kit
we recommend a rotation
speed setting of 10 - 15rpm in tilting rotationmode.

• Method 2 (stirrer with RPM
display): divide RPM settingof stirrer by 40 to obtainpluriPlix® rotation speed, e.g.
600/40 = 15 rpm

07

pluriSelect USA

pluriSelect Worldwide

8912 Bancroft View Drive
San Diego (Spring Valley), CA 91977
USADeutscher Platz 5c04103 Leipzig
Germany

Phone: 619-928-9265
Fax: 619-828 8003
support.usa@pluriselect.com
sales.usa@pluriselect.com

Phone: +49 341 333858-0
Fax: +49 341 333 858 99
support@pluriselect.com
sales@pluriselect.com

www.pluriselect.com

0.1 V