MiSeqTM

Run PCR with a
LymphoTrack
Assay

Purify amplicons

Quantify& prepare
library

PrepareDNAsamples

Unparalleledworkflow simplicity
LymphoTrack Assays put lg/ TCR
next-generation sequencing at your fingertips.
Load & run
MiSeq®

Analyze

Process datawith included
bioinformatics
software

These products are sold for Research Use Only; not for use in diagnostic procedures

Invivoscribe, Inc.
Tel +1 858.224.6600 | Fax +1 858.224.6601
sales@invivoscribe.com

www.invivoscribe.com

Invivoscribe Technologies, SARL
Tel +33 (0) 4.42.01.78.10 | Fax +33 (0) 4.88.56.22.89
sales-eu@invivoscribe.com

MiSeqTM

Workflow Summary
the Master Mix tubes to thaw; then gently01 	Allowvortex to mix.
containment hood or dead air box, pipette 45 µL
02 of	In aMaster
Mix into individual wells of a PCR plate(use a different indexed Master Mix for each sampleand control).

03	Add 0.2 µL EagleTaq™ DNA polymerase to each
Master Mix.

04

the PCR products using the Agencourt AMPure07 	Purify
XP PCR Purification system. When using the LymphoTrack®

®

TRB Assay add 35 µL of particles to each 50 µL reaction,
for all other LymphoTrack Assays add 50 uL of particles toeach 50 µL reaction; elute DNA in 25 µL of eluant.

08	
Quantify amplicons using the KAPA™ libraryquantification kit according to the kit instructions.
Dilute amplicons 1:4,000 before proceeding to qPCR.	Add 5 µL of DNA (at a minimum of 10 ng/µL) from theunknown samples and controls to wells containing therespective Master Mix reactions then pipette up anddown 5-10 times to mix.

equal amounts of amplicons from samples and09 	Poolpositive and negative controls (do not include the noAdd 5 µL of molecular biology grade water to the05 	wellcontaining the Master Mix for the no template

10 	Denature and dilute the library.
Load 600 µL of denatured and diluted library on the11 	MiSeq
Reagent Cartridge.

control then pipette up and down 5-10 times to mix.

the plate and amplify target DNA using the06 	Seal
following thermal cycler program:template control), dilute 1:1,000 then quantify the libraryusing the KAPA library quantification kit.

®

StepTemperatureTimeCycle195 oC

7 minutes

1

2o

95 C

45 seconds

3o60 C

45 seconds

472 oC

90 seconds

5o72 C

10 minutes

1

615 oC∞112	
Set up a MiSeq® sample sheet using the Illumina®
Experiment Manager or provided Sample Sheet *.csv
file.

13 	Start the MiSeq run.
and visualize the acquired data using the14 	Analyze
LymphoTrack Software for the MiSeq .
®

29x*

®

*LymphoTrack IGHV Leader Somatic Hypermutation Assay (32 cycles).

CATALOG #PRODUCTS

QUANTITY

7-121-0129
7-121-0139
7-121-0009
7-121-0039
7-121-0149
7-121-0089
7-121-0099
7-121-0109
7-121-0119
7-121-0059
7-121-0069
7-122-0009
7-122-0019
7-225-0009
7-225-0019
7-227-0019
7-227-0009
7-500-0009
7-500-0008

LymphoTrack® IGH FR1/2/3 Assay Kit A - MiSeqTM
LymphoTrack® IGH FR1/2/3 Assay Panel - MiSeq TM
LymphoTrack® IGH FR1 Assay Kit A - MiSeqTM
LymphoTrack® IGH FR1 Assay Panel - MiSeqTM
LymphoTrack® IGH FR1 Assay Panel B - MiSeqTM
LymphoTrack® IGH FR2 Assay Kit A - MiSeqTM
LymphoTrack® IGH FR2 Assay Panel - MiSeqTM
LymphoTrack® IGH FR3 Assay Kit A - MiSeqTM
LymphoTrack® IGH FR3 Assay Panel - MiSeqTM
LymphoTrack® IGHV Leader Somatic Hypermutation Assay Kit A - MiSeqTM
LymphoTrack® IGHV Leader Somatic Hypermutation Assay Panel - MiSeqTM
LymphoTrack® IGK Assay Kit A - MiSeqTM
LymphoTrack® IGK Assay Panel - MiSeqTM
LymphoTrack® TRB Assay Kit A - MiSeqTM
LymphoTrack® TRB Assay Panel - MiSeqTM
LymphoTrack® TRG Assay Kit A - MiSeqTM
LymphoTrack® TRG Assay Panel - MiSeqTM
LymphoTrack® Software - MiSeqTM
LymphoTrack® MRD Software

indices 1-8 (5 sequencing reactions each)indices 1-24 (5 sequencing reactions each)indices 1-8 (5 sequencing reactions each)indices 1-24 (5 sequencing reactions each)indices 25-48 (5 sequencing reaction each)indices 1-8 (5 sequencing reactions each)indices 1-24 (5 sequencing reactions each)indices 1-8 (5 sequencing reactions each)indices 1-24 (5 sequencing reactions each)indices 1-8 (5 sequencing reactions each)indices 1-24 (5 sequencing reactions each)indices 1-8 (5 sequencing reactions each)indices 1-24 (5 sequencing reactions each)indices 1-8 (5 sequencing reactions each)indices 1-24 (5 sequencing reactions each)indices 1-8 (5 sequencing reactions each)indices 1-24 (5 sequencing reactions each)
1 CD
1 CD

Storage Conditions: -85˚C to -65˚C (DNA controls may be seperated from kits and stored at 2˚C to 8˚C
These products are sold for Research Use Only; not for use in diagnostic procedures.
©2023 Invivoscribe, Inc. (Invivoscribe®). All rights reserved. The trademarks mentioned herein are the property of Invivoscribe and/or its affiliates, or(as to the trademarks of others used herein) their respective owners. ILLUMINA® and Miseq® are registered trademarks of Illumina, Inc. AGENCOURT® and AMPURE®
are registered trademarks of Beckman Coulter, Inc. EAGLETAQ™ is a trademark of Roche. KAPA™ is a trademark of Kapa Biosystems.

M-0045 Rev03 April 2023