C-Kit Education / Space
Protein Crystallization Experiment Kit Series
C-Kit Education/Space are active learning kits for high school
students or higher on the theme of protein crystal growth. Inthe experiment, protein crystal growth is caused by specificsalt concentration. Success of these experiments is to obtain
beautiful crystals which are visible and memorable forstudents. C-Kit Space will make students getting closer to theInternational Space Station (ISS).
“C-Kit Education” is an experimental kit forscience literacy education in class. It enables
students to investigate protein crystallization
experiments with the goal of obtaining highquality crystals.
"C-Kit Space“, an advanced kit, provides
students the opportunity to take part in space Lysozyme crystal
experiment to crystallize their samples underthe microgravity in ISS. This experiment can be
implemented with the results obtained by theformer kit.
(The microgravity environment with little disturbanceis suitable for growing high-quality protein crystals.)“C-Kit Education”, which is a science Literacy Learning Kit,
consists of “Crystal Preparation Kit” (CRT130-1E) and“Crystal Observation Kit” (CRT130-3E).
The
CrystalPreparation
Kit
contains all experimental tools andreagents : NaCl solution, KClsolution,buffersolution
andLysozyme, a stable anti-bacterial
protein. It enables to investigate
the effects of 6 salt solutions atdifferent
concentrations.
The
purpose of this kit is learning how
to design experiments, how to mix
reagents, how to observe andCRT130-1E
discuss the results.

C-Kit Education / Space
Protein Crystallization Experiment Kit Series
The kit includes a detailed instruction with web videos forstudents, and a teaching material for teachers to lead theactive learning successful. The teaching material shows
micrographs of the experimental results with the crystallization
reagents at various concentrations. Crystal Observation Kit
contains a light and a loupe to observe crystals without amicroscope.
“C-Kit Space” is a teaching material for the “space experimentalclass ( https://www.asmilla.com/en-universe/detail/?id=2010 )”held by Manned Space Systems Corporation, Japan (JAMSS).
This class consists of a lecture on the space environment byexperts, and sample preparation for space experiment. The
crystallization samples are loaded onto a rocket andtransported to ISS. High-quality crystals are grown on the ISS
for a month and then returned to the students.
By watching the live streaming (NASA TV) of launching rocket
carrying their samples, students will be motivated to participate
in science and technology development in the future.
C-Kit Space
Exposure to cutting-edge
science and technology

C-Kit Education
Science literacy study
CRT130-1E : JPY 35,000CRT130-3E : JPY 3,100Suggested retail price(excluding tax and shipping fees)

Additional fee is required
for the space experiments.
Please contact to JAMSS.

Products / General Information

Information for space experiment

Fukasawa 5-14-15, Setagaya-ku
Tokyo, 158-0081 Japan
TEL +81-3-5809-1561
FAX +81-3-6411-6261
MAIL : info@confsci.co.jp

Ohtemachi Bld. 8F, Ohtemachi 1-6-1,
Chiyoda-ku Tokyo, 100-0004 Japan
TEL +81-3-3211-2060

Confocal Science Inc.

http://www.confsci.co.jp/

Japan Manned Space Systems Co.

https://www.jamss.co.jp/space_utilizat
ion/kirara/

The contents of this catalog are subject to change without notice. (Ver.2312_1)

C-Kit Education

For science literacy study

C-Kit Education （ CRT130-1E/3E ） is an experimental kit forgroup learning on the theme of protein crystal growth.
Students investigate the effect of crystallization reagents(NaCl or KCl) on lysozyme crystal growth by the counter
diffusion method*. Through this experiment, students candevelop their scientific literacy by designing the experiment,
mixing reagents, performing the accurate manipulation,observing crystals, and discussing the results, on their own.
(The kit includes a detailed instruction with web videos forstudents, and a teaching material for teachers.)

Protein solution
Crystals
Crystallizationreagent

Crystallization
reagent diffuses
into the protein
solution

Crystallization* Mechanism of crystallization using the counter diffusion method
The crystallization reagent slowly diffuses into the lysozyme solution, andgenerates various forms of crystals or precipitate due to the crystallization
reagent.
The lesson in classroom is given as follows.
(This is not the only way.)
1. Each group selects NaCl or KCl as the reagents, that is the main
research subject of this experiment.
2. Discuss an experiment to determine the best concentration of salt togrow good Lysozyme crystals (4 conditions). Create a mixing protocol
for reagents for the conditions.
3. Conduct the crystallization experiments, and observe the crystal
growth on days 7 and 14.
4. Determine the concentration of salt that grows large, well-shaped
crystals (high-quality crystals), and then conduct experiments using
both NaCl and KCl at the same concentration.
5. Observe the crystal growth on days 7 and 14 to confirm thereproducibility and discuss the difference in effectiveness between
NaCl and KCl.
6. Presentation and discussion on the obtained results.

C-Kit Education
Crystal Preparation Kit

Name
Suggestedretail price

35,000 yen(transportation fee, tax,not included)

Model

CRT130-1E

No.

Product nameQty.

Description

1Capillary (φ0.7 mm×47 mm)

6Create crystals inside this. Both ends are round cut.

2

Gel tube (φ1.0 mm×1 cm)

6

Place between protein solution and crystallization
reagent. Soaked in 50 mM acetate buffer.

3

Space grade lysozyme 25 mg/mL,ca. 0.1 mL (for 6 capillaries)

1

Be sure to collect the protein solution at the bottom of thetube. Dissolved in 50 mM acetate buffer.

4salt solutions for crystallization
reagents 4 mL

5250 mM acetate buffer 4 mL

1

For Lysozyme crystallization, crystallization solutions
containing 50 mM acetate buffer (pH 4.5) are required.

6

Uｌtrapure water １４ mL

1

For reagent dilution

7

sealing compound

1

Used to seal the capillary end

8Screw cap tube

8

6 for prepare crystallization reagent and 2 spare tubes

9

1 mL syringe

12

2 for salt, 1 for buffer, 6 for crystallization reagent, 1 forultrapure water, 2 spare syringes

10

Syringe tip parts

12

Use by attaching it to the tip of the syringe

11

Clear round tube (5 mL)

6

For crystal growth observation

12

Paper test tube stand1

For reagent preparation and crystal growth observation

13

1 pair of polarizing plates

１

For crystal growth observation

14Instructions

1

1 each 2 M NaCl, KCl to prepare crystallization reagents

Crystal Observation Kit

Name

Suggested 3,100 yen (transportationModelretail pricefee, tax, not included)
No.

Product name

CRT130-3E

Qty.

Description

1

Loupe

1

Observe crystals from above

2

LED push light

1Crystals sandwiched between polarizing plates areirradiated from below.

3AA battery

3For push light

Products / General Information

Confocal Science Inc.

Fukasawa 5-14-15, Setagaya-ku Tokyo, 158-0081 Japan
TEL +81-3-5809-1561 FAX +81-3-6411-6261 MAIL : info@confsci.co.jp

http://www.confsci.co.jp/
The contents of this catalog are subject to change without notice. (Ver.2312_1)