1st AIST International Imaging Workshop
January 19-25, 2014
Biomedical Research InstituteNational Institute of Advanced Industrial Science and Technology (AIST)
1-1-1 Higashi, Tsukuba, JapanThe 1st AIST International Imaging Workshop is a residential one-week course held at the Biomedical ResearchInstitute, AIST offering a wide range (from the principles of light microscopy to spectral imaging) of microscopy training
to young talented researchers. Participants will be trained through hands-on practical sessions in several critical andimportant issues related to imaging technologies.

Who can join?
The course is suitable for both beginners and experienced users wanting to gain a greater understanding of themicroscope. Students are selected through recommendation from their supervisor internationally from a range ofbackgrounds. Preference will be given to doctoral level students.

Language?
The course, including the course materials, will be conducted in English.
The course will have a maximum of 12-15 delegates.

Support?
Selected candidates will be provided (1) Airfare return by economy class through the most direct route from theinternational airport in their home country to Japan, (2) Bus fare from Narita International airport to Tsukuba Center,(3) Single room accommodation at the AIST Guest House or Hotels around and (4) Allowance to cover the daily
meals.

Course Details
Laboratory Course 1: Basic training in Microscopy
Anatomy of the light microscope, Basic Optics, Lenses, Bright field, Phase
contrast, DIC, Fluorescence, Fluorochromes and Filters, Fluorescenceimaging, Basic microscope maintenanceLaboratory Course 2: Basic training in Laser Scanning Microscopy
Learn the basic technique for a confocal microscopy
Laboratory Course 3: Training in Multi-photon Laser Microscopy
Observation of a deep brain layer by using two-photon
Photo activation,Calcium imaging after laser stimulation
Laboratory Course 4: Training in Spectral confocal & Super-resolution
Microscopy
Separation of cellular components from four-color fluorescent-labeled cells.
Learn the technique for super-resolution observation

ProgramJanuary 19, 2014 Arrival and Reception
January 20, 2014
Course 1- Basic training in Microscopy
Course 2: LSM(All members)
January 21, 2014 ~ January 23, 2014
Course 3: Multi-photon Laser Microscopy
Course 4: Spectral & Super Resolution
Microscopy
Course 5: Bioluminescence Screening &Imaging*Course 3- 5 will be held with a group of 3-5
members by rotation

Laboratory Course 5: Training in Bioluminescence Screening & Imaging
Learn the principle and techniques in high throughput screening & cell
imaging based on bioluminescence technology

January 24, 2014 Tour to imaging or life
sciences companies in and around Tsukuba

Several lectures on the novel techniques of microscopy will be given (TBA)

January 25, 2014 Farewell and Departure

Instructors: Y. Ohmiya, Y. Onishi, K. Katoh, T. Ebihara, T. Ochiishi, M. Doi, T. Mizuno and A. Nagasaki
Organizers: Y. Ohmiya, R. Wadhwa, M. Doi and S. KaulSponsors: Nikon and Olympus
Request for application form and further information should be mailed to: do-m@aist.go.jp or s-kaul@aist.go.jp
Deadline for application: November 15, 2013