Ion S5™/PGM™

Run PCR with a
LymphoTrack
Assay

Purify amplicons

Quantify& prepare
library

PrepareDNAsamples

Unparalleledworkflow simplicity
LymphoTrack Assays put lg/ TCR
next-generation sequencing at your fingertips.

Load & run
Ion S5™/PGM™

Analyze

Process datawith included
bioinformatics

These products are sold for Research Use Only; not for use in diagnostic procedures

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Tel +1 858.224.6600 | Fax +1 858.224.6601
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Ion S5™/PGM™

Workflow Summary
gloved hands, remove the Master Mixes from the01 Usingfreezer. Allow the tubes to thaw; then gently vortex to mix.
containment hood or dead air box, pipette 45 µL
02 Inof aMaster
Mix into individual wells of a PCR plate (useQuantify amplicons with an appropriate method (08 Bioanalyzer
2100 or LabChip GX).

e.g.

®

the library by combining 4 nM of each amplicon
09 Createin a single tube (do not include the no template control).

a different indexed Master Mix for each sample andcontrol).

03 Add 0.2 µL EagleTaq™ DNA polymerase to each Master Mix.
dd 5 µL of DNA (at a minimum of 10 ng/µL) from the04 Aunknown
samples and controls to wells containing therespective Master Mix reactions then pipette up anddown 5-10 times to mix.

5 µL of molecular biology grade water to the well
05 Addcontaining the Master Mix for the no template control

10 Dilute the library.
repare templates using the OT2 or Ion Chef system.
11 P*TheOT2 system is used to perform an emulsion PCR tocreate template-positive ion sphere particles (ISPs) thatmust be further enriched.
Ion PGMIon S5OT2Ion PGM Hi-Q OT2 Kit or Ion
PGM Hi-Q view OT2 Kit or OT2200 Kit if only testing TRG

Ion 520 & Ion 530 Kit— OT2Ion
Chef

Not applicableIon 510 & Ion 520 &Ion 530 Kit — Chef

then pipette up and down 5-10 times to mix.

06

 eal the plate and amplify target DNA using theSfollowing thermal cycler program:Standardized Program

StepTemperatureTimeCycle1295 oC95 oC

7 minutes
45 seconds

1345660 oC
72 oC
72 oC
15 oC

45 seconds
90 seconds
10 minutes∞29x
1
1Purify the PCR products using the Agencourt AMPure
07 XP
PCR Purification system. Add 90 µL of particles to®

each 50 µL reaction; elute DNA in 40 µL of eluant.

®

®

templates: Enrich template-positive ISPs with the12 *OIonT2OneTouch(tm)ES.
the S5 or PGM. Load S5 [Ion 520, Ion 530]
13 Initializeor PGM chip [316 v2 BC, 318 v2 BC] with preparedtemplates.

14 Create a Planned Run using the Torrent Browser.
15 Start the S5/PGM run.
nalyze and visualize the acquired data using the16 ALymphoTrack
Software for the S5/PGM.

Ordering Information
CATALOG #PRODUCTS

QUANTITY

7-121-0057

LymphoTrack IGH FR1/2/3 Assay - S5/PGM™

12 indices - 5 sequencing reactions each

7-121-0007

LymphoTrack® IGH FR1 Assay - S5/PGM™

12 indices - 5 sequencing reactions each

7-121-0037

LymphoTrack IGH FR2 Assay - S5/PGM™

12 indices - 5 sequencing reactions each

7-121-0047

LymphoTrack IGH FR3 Assay - S5/PGM™

12 indices - 5 sequencing reactions each

7-122-0007

LymphoTrack IGK Assay - S5/PGM™

12 indices - 5 sequencing reactions each

7-227-0007

LymphoTrack TRG Assay - S5/PGM™

12 indices - 5 sequencing reactions each

7-500-0007

LymphoTrack® Software - S5/PGM™

1 CD

7-500-0008

LymphoTrack MRD Software

1 CD®

®
®
®
®

®

Storage Conditions: -85 °C to -65 °C (DNA controls may be separated from kits and stored at 2 °C to 8 °C).
These products are sold for Research Use Only; not for use in diagnostic procedures.
©2023 Invivoscribe, Inc. (Invivoscribe®). All rights reserved. The trademarks mentioned herein are the property of Invivoscribe and/or its affiliates, or (as to the trademarks of othersused herein) their respective owners. Hi-Q™, ONETOUCH™, and Ion Chef, Ion 316™, Ion 318™, Ion 510™, Ion 520™ and Ion 530™ are trademarks of Thermo Fisher Scientific or its
subsidiaries. AGILENT® and BIOANALYZER® are registered trademarks of Agilent Technologies, Inc. AMPURE® is a registered trademark of Beckman Coulter, Inc. LABCHIP® is aregistered trademark of Perkin Elmer. EAGLETAQ™ is a trademark of Roche.
M-0043 Rev 03 April 2023