Density MediaSpecial Application

Double Gradient Centrifugation

Size

Order No.

100 ml60-00091-10

250 ml60-00091-11

500 ml60-00091-12

Density Gradient Media

Size

Order No.

www.pluriselect.com

100 ml60-00092-10

250 ml60-00092-11

500 ml60-00092-12

Size

Order No.

100 ml60-00093-10

250 ml60-00093-11

500 ml60-00093-12

Size

Order No.

1,000,000

100 ml60-00094-10

250 ml60-00094-11

500,000

Combine PBMC and Leuko Spin Medium

500 ml60-00094-12

The combination of different Spin Media increases your possibilities for sample preparation with density centrifugation. A blood sample can be split into PBMC (Fig. 9)
and granulocytes (Fig. 10) within one centrifugation step. This method allows you
to access untouched granulocyte enrichment with high yield and purity.

Leuko Spin Medium

PBMC Spin Medium(Lympho Spin Medium)

PBMC 24+ Spin Medium(Lympho 24+ Spin Medium)Fig. 8: Layers after double gradientcentrifugation.

PLT Spin Medium

02,000,000

4,000,000

6,000,000

FSC - A

Fig. 9: Enriched PBMC fraction withdouble gradient centrifugation*

00500,000

SSC - A

SSC - A

1,000,000

1,600,000

1,600,000

Fig. 7: Preparation of double gradient

02,000,000

4,000,000

pluriMate

6,000,000

FSC - A

Fig. 10: Enriched granulocyte fraction(up to 97%) with double gradientcentrifugation**Flow cytometry analysis was gated on CD45+ cells.

Advantages using a double GradientTubes to support the density centrifugation process.
Tube SizeSample Volume

Order No.

pluriMate-22 ml

0.25 - 1 ml

44-00002-10

pluriMate-1515 ml

1 - 12 ml

44-00015-10

pluriMate-5050 ml

5 - 20 ml

44-00050-10✓ CTC enrichment in combination with pluriSpin CD45depletion

pluriSelect Life Science

pluriSelect USA

pluriSpin CD15 depletion or eosinophil granulocytes in

Deutscher Platz 5c04103 Leipzig
Germany

Spring Valley,CA 91977
USAcombination with pluriSpin CD16 depletion

Phone: +49 341 333858-0

Phone: 619-202-4297
support.usa@pluriselect.com✓ Fast enrichment of untouched granulocytes✓ Access to basophil granulocytes in combination with

✓ Multiple cell fractions within one centrifugation step

Global Headofficesupport@pluriselect.com

www.pluriselect.com/products/density-gradient-centrifugation.html
V 1.2

DataWorkflow
1,600,000✓ Enrich cells with high yield and a maximum viability

1,600,000

Facts & Features

Whole Blood
SSC - A

SSC - A

500,000✓ No training or special equipment required

500,000

centrifugation

PBMC Spin

1,000,000

1,000,000✓ Usable with standard protocols for density gradient✓ Isolate from whole blood, buffy coat or cord blood0

Mononuclear cells (PBMC)

PBMC 24+ Spin Medium(Lympho 24+ Spin Medium)

Mononuclear cells from8 - 48 hours old peripheral blood

PLT Spin Medium

Platelets

1,600,000

1,600,000

1,000,000

SSC - A
500,000

500,000

0002,000,000

4,000,000

6,000,000

Fig. 3: Enriched white blood cells with Leuko
Spin Medium*pluriMate® centrifuge with brake onand pure of sample after centrifugation
into a fresh tube.

4,000,000

6,000,000Fig. 4: Enriched platelets with PLT Spin Medium*

The major white blood cell populations of whole blood (Fig. 1) with interest for
research and development are lymphocytes (black), monocytes (pink), granulocytes(blue) and platelets (green). The usage of the Spin Media allows to enrich thedifferent cell populations for a wide range of downstream applications.

PBMC 24+ Spin Medium - access to old blood - improve your results

1,000,000

PBMC Spin

PBMC 24+ Spin

500,000

500,000

SSC - A

SSC - ApluriMate® avoids the mixing of sample
and density media while overlaying.

2,000,000

FSC - A00

pluriMate® easy and fast handling ofsample preparation with density media.

0

FSC - APBMC (peripheral blood mononuclear cells), PMNC (polymorphonuclear cells)

pluriMate®

6,000,000

PLT Spin

1,600,000

PBMC Spin Medium(Lympho Spin Medium)

4,000,000

1,000,000

All Leukocytes (PBMC, PMNC,granulocytes)

2,000,000

Fig. 2: Enriched PBMC with PBMC Spin Medium*

Leuko Spin

1,600,000

Leuko Spin Medium

0FSC - A

SSC - A

✓ Usage of different Spin Media with double density

Enriched Cell Population

6,000,000

1,000,000

separation

Spin Medium

4,000,000

Fig. 1: Whole blood, major cell populations*✓ Usable for sample preparation for magnetic cell

Available Density Mediafor human cell enrichment

2,000,000

FSC - A

with pluriSpin negative cell separation

gradient centrifugation for special applications

00✓ Enrichment of untouched specific cells in combination

02,000,000

4,000,000

6,000,000

FSC - A

Fig. 5: Enriched PBMC with PBMC Spin Medium
from blood 24 hours after blood donation*

02,000,000

4,000,000

6,000,000

FSC - A

Fig. 6: Enriched PBMC with PBMC 24+ Spin
Medium from blood 24 hours after blood
donation*

Most density gradient media are limited for the use of fresh whole blood. PBMC 24+Spin Medium is recommended for the use of whole blood that is older than 8 hours.
This medium helps to reduce the time depended contamination of the PBMC fraction
with granulocytes and debris (see Fig 5 and Fig. 6).
*Flow cytometry analysis was gated on CD45+ cells.