★★★ ＜第30回知的財産翻訳検定試験【第16回和文英訳】＞ ★★★
≪１級課題 -バイオテクノロジー-≫【問 1】
Swine that have been infected with the virus in the field develop symptoms
irrespective of their breed, age, and sex, and the season, and many of theinfected swine die. In Japan, classical swine fever occurred for the firsttime in 1888; thereafter, outbreaks recurred nationwide and caused greatdamage to pig farming. Although the application of live vaccines hassuppressed epidemics of the disease since 1969, sporadic occurrence of thedisease has been observed.
The types of symptoms include: a diarrhea type in which bovines show
symptoms of diarrhea with bloody stool, fever, and anorexia; a fever typecharacterized by diphasic fever and leucopenia; a mucosal disease type inwhich bovines show cold-like symptoms such as fever, anorexia, cough, andsialorrhea in the early stage of infection, followed by watery diarrhea anddehydration, leading to death in 1-3 weeks; and a fetal infection type inwhich fetuses are infected in pregnant bovines, which causes stillbirth orresults in termination of pregnancy due to death of the fetuses. In Japan,
bovine viral diarrhea-mucosal disease occurs nationwide and causes greatdamage to cow farming.

【問 2】
Primers which hybridize specifically to a partial region under suchconditions preferably have a nucleotide sequence completely complementary
to the partial region. However, even if primers having a sequence in which
10% or less of the nucleotides are substituted are used, in general, theyhybridize to the target partial region and amplification occurs in many cases.
As such, forward primers that specifically hybridize to a partial region
within the region of nucleotides 1 to 3476 in the nucleotide sequence shown
in SEQ ID NO: 1 include primers consisting of a nucleotide sequence of 15 ormore contiguous nucleotides, preferably 18 or more contiguous nucleotides
within the region of nucleotides 1 to 3476 in the nucleotide sequence shownin SEQ ID NO: 1, primers consisting of a nucleotide sequence in which 10%
or less of the nucleotides in said nucleotide sequence are substituted, andprimers having an additional sequence on the 5' terminus of these primers.
That is, the forward primer may have, in the 3' terminus of the primer, anucleotide sequence of 15 or more contiguous nucleotides within the region ofnucleotides 1 to 3476 in the nucleotide sequence shown in SEQ ID NO: 1, or a
nucleotide sequence in which 10% or less of the nucleotides in said nucleotide
sequence are substituted.

【問 3】
After the culture was completed, the medium in each well was replaced withsclerostin-free medium. Then a portion of the cells attached to the bottomof each well was stripped off in a constant width using a pipet tip. After thestripping, to remove floating cells including the stripped cells, the medium ineach well was replaced with the sclerostin-free medium and the dishes wereincubated at 37°C in the presence of 5% CO2. At the start of the culture
after the stripping, at five hours after the start of the culture, and at sevenhours after the start of the culture, images of the spaces formed by thestripping were captured under a phase-contrast microscope. Changes inareas of the spaces were quantitated by measuring, with Image J software,
the areas of the spaces in the captured images. A resulting value wasobtained by subtracting the area after five hours from the area at the start ofthe culture for each well. The average and standard error for each groupwere calculated using the resulting value for each well. The differences
between two groups were tested using Student's t-test.

【問 4】
Claim 1. A transgenic fish comprising, in the genome thereof, a promoter
that requires a ligand-bound AhR/ARNT complex as a transcription factor
for transcription initiation, and DNA encoding a marker protein, whereinsaid DNA encoding the marker protein is under control of said promoter andwherein said marker protein emits a visually detectable signal.
Claim 2. The transgenic fish of claim 1, wherein said fish is Oryzias.

Claim 5. The transgenic fish of any one of claims 1 to 4, wherein expressionof DNA encoding an endogenous protein that degrades or inhibits said ligand
is downregulated.
Claim 6. A method of detecting or quantifying a substance to be detected,
said method comprising placing the transgenic fish of any one of claims 1 to 5
in a liquid to be subjected to the detection, detecting said signal visually, anddetecting or quantifying the substance to be detected based on the results
thereof.
Claim 8. The method of claim 6 or 7, wherein the transgenic fish comprises
an Oryzias egg and said placing is carried out using said Oryzias egg that is
5 days or older after fertilization.