Patchliner
Because quality does matterPatchliner

Most versatile automated patchclamp system on the market
The Patchliner is a fully automated patch clamp

temperature control and dynamic clamp makesystem which records from either 4 or 8 cells

this instrument the most versatile APC system on

simultaneously. The HEKA EPC10 amplifiers, fullthe market. The Patchliner is tried and tested andcontrol over the pipette speed, ability to change

is appreciated in academia, industry and CROs

protocols on-the-fly and flexible add-ons foralike since its introduction in 2006.

Patchliner

Key features at a glance1

Up to 8 cells recorded simultaneously

5

Dynamic clamp (optional)

2

High success rates

6

Fast external application3

Voltage and current clamp as standard

7

Minimized exposure time

4

Sophisticated temperature control

8

Unlimited compound application

Patchliner

Powerful data acquisition
and analysis
PatchControlHT is a graphical user interface

Data Analysis Package for the Patchliner

for straightforward and easy programming ofaccelerates data analysis by automatic loading

protocols and experimental parameters.

of data and EC50 or IC50 calculation.

• Quick and easy set up of experimentalparameters• Based on IgorPro• Transfer voltage protocols from manual setup• Analysis performed with a few mouse clicks(IC50, EC50, Vhalf)• Display of online analysis during experiment• Display of raw data traces facilitates QC• Expert and User modes of operation• Instant recalculation of average plots after
manual QC• Changes on-the-fly• Pool data from different chips and days

PatchControlHT
Color-coding of seal resistance andcompound addition facilitates an easy
overview of the experiment in usermode.

Patchliner

Versatile and flexible:
Research highlights
nAChRα7

1 µM Nic

30 °C

TRPV3

10 µM Nic

34 °C

30 µM Nic

38 °C

100 µM Nic

42 °C

300 µM Nic

46 °C

1 mM Nic

50 °C
54 °C

0 nA

200 pA
5 ms−100 mV

5 nA
100 mV

50 mV

Ligand-gated ion channels

Heat activation

Fast pipetting speed and ability to stack solutions

The sophisticated temperature control can beensures reliable activation of fast desensitizing

used to activate heat activated channels suchligand-gated ion channels such as nicotinic α7

as TRPV3. Solution is heated in the pipette andreceptors. Stacking solutions inside the pipette

rapidly applied to the cell causing rapid andminimizes exposure time by eliminating lengthytransient activation of TRP channels.

pick-up times.

0 free Ca2+

−93 pA constant current

1 μM free Ca2+

200 pS/pF

5 mM BaCl2

300 pS/pF
400 pS/pF
500 pS/pF
600 pS/pF
700 pS/pF

0 mV

00.5 nA

20 mV
50 ms

200 msInternal exchange

Dynamic clamp

The internal solution of the Patchliner can be

The Dynamite8, an add-on for the Patchliner for

exchanged during the experiment. This can be

automated dynamic clamp, facilitating stableused to activate Ca2+-activated channels suchmembrane potential and action potential

as KCa3.1.

duration.

PatchlinerYour research, our passion

Ion channels

Assay development and validation

Ideal for both voltage- and ligand-gated ion

Full flexibility to design assays for a wide variety ofchannels.

cell lines.

Drug Discovery

CiPA validation study

Suitable for all phases of drug discovery inThe Patchliner is delivered fully compliant with thepharma and biotech.

latest CiPA recommended guidelines.

Academic research

Channelopathies

Easy solutions tailored to ensure your next

Sophisticated functional assays for the discovery

scientific breakthroughs.

of ion channel mutations.

CROs

Primary cell/iPSC profiling

Deliver accurate, reproducible and high quality

Low cell consumption for recording primary anddata on time and within budget.

stem cells.

Patchliner

The Patchliner includes• Patchliner Quattro: 4 amplifier channels• Patchliner Octo: 8 amplifier channels• 1–2 HEKA EPC10 Quadro amplifiers• Windows 10 OS with PatchControlHT and PatchMaster software• Patchliner Data Analysis Package (incl. Igor Pro)
• NPC-16 borosilicate recording chips• Reagent starter kit• On-site installation support• 1 year warranty with further optional comprehensive service plans available

Specifications
Average whole-cell stability>30 minutes

Successful whole-cell recordings

70 – 90%

Throughput

250 – 600 data points per day

Seal resistance>1 GΩ

Series resistance<10 MΩ

Chip resistance

Low, medium, high, ultra-high, multi-hole (4X)

Perfusion time constant<20 msMinimum exposure time

200 msAmplifier channels

4 or 8Temperature control

Up to 60 °C (heating only)

Current clamp

Integrated as standard

CoolingPlate

Cooling of cell storage and compounds(optional)

accelerate your researchnanion Europe
info@nanion.dephone: +49 89 2190 95 - 0
www.nanion.denanion USA
info@naniontech.com
phone: 1-888-9-NANION
www.naniontech.com
nanion China
andy.di@nanion.cn
phone: +86 10 82 17 6388
www.nanion.cn
nanion Japan
info@nanion.jp
phone: +81 3 6457 8773
www.nanion.jp

To find your nearest Nanion
representative, visit our website:www.nanion.de