Custom Oligo ＤＮＡ Synthe
esisO���� ��� ��n������（�n���dd�n� ����������d O����）�r�d��� ��n�������������� ��n���������r��������nScale

0.03μmol
Desalted

0.03μmol
Cartridge-Purified

0.05μmol

0.2μmol

1.0μmol

Price(/mer)

¥50

¥70

¥90

¥150

¥400

Desalt

¥0-

¥0

¥0

¥0

Cartridge-Purified-¥0¥1,000¥2,000¥4,000HPLC-Purified--¥4,000¥5,000¥10,000

Expected Yield(20mer Desulted)

5 O.D.

3 O.D.

15 O.D.

25 O.D.

80 O.D.

mer Number Limit～40mer～40mer～80mer～90mer～120mer* The delivery fee is free if purchase of over 1 tube of 0.03μmol Desulted Oligo.
* If the purchase of Oligo less than 15mer, the price is the same as the 14 mer oligo regardless of less than 15 mer Oligo.
* BEX will propose more scale and/or more purification according to difficu
ulty of oligo sequence and/or modification.
* The yields depend on the oligo sequence and/or modification.

������r� ����O��n ����Un��� ����������� Ord�r� ������ Ord�r
U ��� �����Un����������������� Ord�r
O dDelivery Date

1 BusinessDay

2 BusinessDay

Desulted～40mer

Cartridge～40mer

Desulted～49mer～80mer

Cartridge～49mer～80mer

3 Buusiness
DDay

4 BusinessDay

5 BusinessDay� BusinessDay～49mer～80mer～49mer～90mer7 BusinessDay

0.03μmol

0.05μmol

HPLC

0 2μmol
0.2μmol

Desulted～90mer

Cartridge～90merHPLC

1.0μmol

Desulted～120mer

Cartridge～120mer

HPLC～49mer～120mer* The order date is 0 business day. (Saturday, Sunday, and Holiday aren’t counted.)* The delivery date depends on the number in one order and modification for oligo.

BEX CO.,LTD.

5

http�//www.bexnet.co.�pe-mail info�bexnnet.co.�pphone �81-3-5375-1071

fax �81-3-5375-5�3�

Custom Oligo ＤＮＡ Syntheesis������� ��������� ����Free for Mix Base＜Mix Base List＞
��SDBN

�

M

�

H

�

I

A,G

A,T

C,G

A,G,T

C,G,T

A,C,G,T

C,T

A,C

G,T

A,T,C

A,C,G

inosine* Additional fees for the change of the rate for mix base, 3’Mix base, andd inosine�������
Scale

0.05μmol

0.2μmol

1.0μmol～5 points × 1 tube

¥8,000¥8,000¥10,000～5 points × 2 tube

¥6,000¥6,000¥8,0005 ～ Points
3’ Inosine¥1,000 addded per additional 1 Inosine¥8,000-¥12,000���������
AAnnealingli Oligo
Oli DNAs
DNA synthesized
th i d andd cartridget id and/ord/ HPLC purififfied
fi d iin BEX
BEX.
Scale

0.05μmol

0.2μmol

1.0μmol

Annealing / 1set of Double Strand¥5,000¥5,000¥5,000Desulted（After Annealing）
HPLC Purification (After Annealing）¥0¥4,000¥5,000¥10,000* Additi
Additionall ffee ffor S
Synthesisth i andd Purification
P ifi ti off single
i l strandedt d d Oligo
Oli DNAD�-����� ��� ������������������S-Oligo DNA is Oligo DNA whose phosphate groups between nucleotides are phosphorothioated. This Oligo DNA is so tolerant
aagainst most nucleases that they are used for the experiments of anti-sense.
Scale

1.0μmol

2.0μmol～

Set-Up Fee¥3,000-Price （/mer）¥400¥200 for Additional 1.0μmol

Cartridge Purification¥4,000¥2,000 for Additional 1.0μmol

HPLC Purification¥10,000¥5,000 for Additional 1.0μmol

��-����
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Scale

0.03μmol Desultedd

0.03μmol Cartridge Purification

Price （/mer）

¥45

¥65

mer Number Limit～35mer～35mer* This service is acceptable for 96 Oligos at once.
* The delivery date is a week for 96 Oligos in one plate.
* The mix base is acceptable (Additional fee for 3’
3 Mix base).
base)* Oligo with less than 15mer is applied for the fee of 14 mer Oligo.
* Inosine, modification, and specialized Oligo are acceptable for 1-tube tyype Oligo.

BEX CO.,LTD.

http�//www.bexnet.co.�pe-mail info�bexnnet.co.�p

phone �81-3-53�5-10�1fax �81-3-53�5-5636

6

Custom Oligo ＤＮＡ Syntheesis
2TQFWEV.KPGWRÞ'PF ᲭÞ'PF/QFKHKECVKQPModification fee is applied for each tube. The volume discounts are appliedafor over 1 tube with the same 5’ end modification in
one order like below. With one tube oligo 5’ end and 3’ end modifiedd, the fee will be combined.
5’ end Modification
5’Cy3※
5’Cy5※
5’FAM
55’HEX
HEX
5’TET
5’ROX（TEXAS RED）
5’TAMRA（Rohdamine）
5’Biotin
5’Amino5’Phosphorylation
5‘Thiol
5’DIG

33’ End Modification

1-4 tube�s�

5-9 tubes

0 05 or 00.2
0.05
2

¥15 000¥15,000¥14 500¥14,500¥14 000¥14,000

1.0¥30,000¥29,000¥28,000

0.05 or 0.2¥15,000¥14,500¥14,000

1.0¥30,000¥29,000¥28,000

0.05 or 0.2¥10,000¥8,000¥7,0001.0¥15,000¥12,000¥10,500

0.05 or 0.2¥10,000¥8,000¥7,0001.0¥15,000¥12,000¥10,500

0.05 or 0.2¥10,000¥8,000¥7,0001.0¥15,000¥12,000¥10,500

0.05 or 0.2¥12,000¥9,600¥8,4001.0¥20,000¥16,000¥14,000

0.05 or 0.2¥12,000¥9,600¥8,40010
1.0¥20 000¥20,000¥16 000¥16,000¥14 000¥14,000¥10,000¥8,000¥7,0001.0¥20,000¥16,000¥14,000

0.05 or 0.2¥5,000¥4,000¥3,5001.0¥10,000¥8,000¥7,0000.05 or 0.2¥5,000¥4,000¥3,5001.0¥10,000¥8,000¥7,0000.2¥15,000¥12,000¥10,500

1.0¥25,000¥20,000¥17,500

0.05 or 0.2¥15,000¥12,000¥10,500

1.0¥25,000¥20,000¥17,500

Scale※ About Cy3,Cy5 oligos:

0.2�mol

1.0�mol

3’Cy3※-¥30,000¥60,000

3’Cy5※-¥30,000¥60,000

3’FAM¥20,000¥20,000¥30,000

3’FITC¥20,000¥20,000¥30,000

3’ROX（TEXAS RED）-

¥20,000¥35,000

33’TAMRA（Rohdamine）
TAMRA（Rohdamine）-¥20,000¥35,000

3’Biotin¥20,000¥20,000¥30,000

3’Amino-¥15,000¥30,000¥10,000¥10,000¥18,000

3‘ThiolS-S-¥45,000¥50,000

3’DIG-¥20,000¥30,000

BEX CO.,LTD.

10 - tubes

0.05 or 0.2

0.05�mol

3’Phosphorylation

7Scale ��mol

http:��www.bexnet.co.�pe-mail info�bexnnet.co.�p

The purchase of this Product includes alimited non-exclusive sublicense under U.S.
Patent No. 5,268486 and any continuation,
continuation-in-part, re-examination,
divisional, reissue or foreign equivalent, touse the Compounds in the Product toperform research. NO OTHER LICENSE IS
GRANTED EXPRESSLY, IMPLIEDLY OR BY
ESTOPPEL. Use of the Product forcommercial purposes is strictly prohibitedwithout written permission from GE
Healthcare Bio-Sciences Corp.
For information concerning availability ofadditional licenses to practice the patented
methodologies, please contact GE Healthcare
Bio-Sciences Corp.

phone �81-3-5375-1071

fax �81-3-5375-5636

Custom Oligo ＤＮＡ Syntheesis
2TQFWEV.KPGWR
5RGECKN /QFKHKECVKQP
We accept the synthesis of specially modified oligos. Some of our performancepis listed blow. Please ask us.
The List of Our perrforamance
Amino Modifier C6-dT

Amino Modifier C3

AAminoModifier C12

Amino C6(MMT)

PC Amino Modifier

Biotin-dTF
Fluorescein-dT

DabcylDabcyl-dT

5’BHQ-1

B
BHQ-1-dT

5’BHQ-2

BHQ-2-dT

D-spacer

SSpacer
9Spacer 18

8-Br-dA

5-Br-dA

55-Br-dU

5-I-dC

5-I-dU

5-F-dU2
2’3’ddA

2’3’ddG

2’3’ddC

2’3’ddT

7
7-deaza-dA

2’-Aminopurine

Etheno-dA

5-Nitoroindole

33-Nitoroindole

5’Psoralen(C2)5’Psoralen(C6)Puromycin

33’Cholesteryl-TEG

Cholesteryl-TEG

DNP-TEG

Glyceryl-CPG

ddeoxypseudo-U

Dithiol

5-Me-dC

3’-5-Me-dC

006-Me-dG

N6-Me-dA8-oxo-dA8-oxo-dG

55-OH-dC

5-OH-dU

5-Hydroxymethyl-dU

Tymin-Dimer

T
Thiol
S-S

etc

&GNKXGT[2TQFWEV
1tube type・Dried up or Diluted in D.W. or TE
・Datasheet with the sequence, M.W., Tm, the rate of GC, O.D., μg, nmol・HPLC Chart （Applicable product only）
96-well plate type・Dried up or Diluted in D.W. or TE・Cluster tube plate: 1.2ml
・Datasheet with the sequence, M.W., Tm, O.D., nmol, the amount off solution（Diluted only） / well position(NQYVQQTFGTPlease send your requests to us.

I: Please emaail or send the order form downloaded from BEX web page byemail. (http://www.bexnet.co.jp)
Emails with the information of the oligo sequence and any other data are
FAX : +81-3-5375-5636
acceptable. E-mail : info@bexnet.co.jp
II: Web forms are also acceptable.

FAQ
Q.Some white crystal is included in the oligo tube. Is this aproblem?
A.Dried-up ligo DNA usually forms 3 types:
1. white crystal
2. clear pellet
3. clear film
Even the same sample will form different from what it was as it isdried up. In any cases, the quality is no problem.

Q.How to keep oligos
A Dried-up
A.Driedup oligos are reserved in the refrigerator (4℃) or the freezer(－20℃) until use. Diluted oligos are cryopreserved. We strongly
recommend that oligos are cryopreserved after dispensed because
the repeat of melting and freezing dramatically lowers the quality.

Q.How is oligo diluted
A.If 100pmol/ul oligo is needed, dilute oligo with the amount (uL) 10
times as much as the amount (uL) value equivalent to “nmol” inthe datasheet delivered together.

BEX CO.,LTD.

http://www.bexnet.co.jp

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

8

Custom RNA Synthesis����� ��� ���������������
������� ����������� ���������
0 2μmol
0.2μmol

1 0μmol
1.0μmol

Scale・Purification
Price (double strands set)

Cartridge

HPLC

Cartridge

HPLC¥20,000¥35,000¥50,000¥80,000

50nmol15nmolChain Length（incl. overhang）

21～23merYield

20nmol5nmol* overhang is applied for dTdT or ｒUrU.
ｒUrU* The listed price applies for 21-23mer（incl. overhang）.
* The listed yield applies for siRNA with no modification and the yield at th
he point of the delivery.

��� ���������Scale

0.2μmol

1.0μmol

Price (/mer)¥2,000¥2,900HPLC Purification¥10,000¥15,000* The fee of Less than 15-mer RNA is applied for one of 14-mer RNA.
* Our staff will contact you about the scale and yield in advance according to the difficulty of synthesis.
* The yield depends on the sequence.

�������� ����Our staff contacts you after the order.

�������� �������
siRNA Synthesis
・Freeze-dried
・Freezedried （Frozen）
・Datasheet Sequence / M.W. / Tm / GC% / μg / nmol
・ The photos of Electrophoresis
・TOF-MASS data
・ HPLC Chart（HPLC order only）
RNA Synthesis
・Freeze-Dried （Frozen）
・Datasheet Sequence / M.W. / Tm / GC% / μg / nmol・HPLC Chart���� �� �����

Please send your requests to us.

BEX CO.,LTD.

9

http://www.bexnet.co.jp

Please email or send the order form downloaded from BEX web page byemail. (http:///www.bexnet.co.jp)
Emails with thhe information of the sequence and any other data are
acceptable E
acceptable.
E-mailmail : info@bexnet.co.jp
info@bexnet co jp
FAX : +81-3-5375-5636
81 3 5375 5636

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636