原著論文

1.

corresponding author の場合 *Weidong Zhang, Ayako Miura, Md Moin Abu Saleh, Koichiro Shimizu, Yuichiro Mita, Ryota Tanida, Satoshi
Hirako, Seiji Shioda, Valery Gmyr, Julie Kerr-Conte, Francois Pattou, Chunhuan Jin, Yoshikatsu Kanai, Kazuki
Sasaki, Naoto Minamino, Hideyuki Sakoda, Masamitsu Nakazato. The NERP-4-SNAT2 axis regulates pancreatic
β-cell maintenance and function. Nature Communications 14: 8158, 2023.

2.

Durga Paudel, Yasuhiro Kuramitsu, Osamu Uehara, Tetsuro Morikawa, Koki Yoshida, Sarita Giri, Syed Taufiqul
Islam, Takao Kitagawa, Tadashi Kondo, Kazuki Sasaki, Hirofumi Matsuoka, Hiroko Miura, Yoshihiro Abiko:
Proteomic and microbiota analyses of the oral cavity during psychological stress. PLoS ONE 17: e0268155, 2022.

3.

Shingo Miyamoto, Yoshiko Nagano, Makoto Miyazaki, Yuko Nagamura, Kazuki Sasaki, Takeshi Kawamura,Kazuyoshi Yanagihara, Toshio Imai, Rieko Ohki, Masakazu Yashiro, Masato Tanaka, Ryuichi Sakai, Hideki
Yamaguchi: Integrin α5 mediates cancer cell-fibroblast adhesion and peritoneal dissemination of diffuse-type
gastric carcinoma. Cancer Letters. 526: 335-345, 2022.

4.

Yuko Nagamura, Makoto Miyazaki, Yoshiko Nagano, Masako Yuki, Kiyoko Fukami, Kazuyoshi Yanagihara,
Kazuki Sasaki, Ryuichi Sakai, Hideki Yamaguchi: PLEKHA5 regulates the survival and peritoneal disseminationof diffuse-type gastric carcinoma cells with Met gene. Oncogenesis 10: 25, 2021.

5.

Satsuki Miyazaki, Aya Tashiro, Takashi Tsuchiya, Kazuki Sasaki, Jun-ichi Miyazaki. Establishment of a longterm stable β-cell line and its application to analyze the effect of Gcg expression on insulin secretion. Scientific
Reports 11: 477, 2021

6.

Takashi Tsuchiya, Aya Nakayama, Takeshi Kawamura, Kazuki Sasaki*. Capillary electrophoresis electrosprayionization-mass spectrometry for peptidomics-based processing site determination. Biochemical and BiophysicalResearch Communications 533: 872-8, 2020

7.

Kazuki Sasaki*, Takashi Tsuchiya, Tsukasa Osaki. Isolation of Endogenous Peptides from Cultured
Cell Conditioned Media for Mass Spectrometry. Methods in Molecular Biology 1719:51-8, 20188.

Takashi Tsuchiya, Hiroshi Iwakura, Naoto Minamino, Kenji Kangawa, Kazuki Sasaki*. Endogenous peptide
profile for elucidating biosynthetic processing of the ghrelin precursor. Biochemical and Biophysical ResearchCommunications 490:1142-46, 20179.

Cherl Namkoong, Koji Toshinai, Zaved Waise, Hideyuki Sakoda, Kazuki Sasaki, Yoichi Ueta, Min-Seon Kim,
Naoto Minamino, Masamitsu Nakazato. NERP-2 regulates gastric acid secretion and gastric emptying via theorexin pathway. Biochemical and Biophysical Research Communications 485:409-13, 201710.

Soon Gang Choi, Qian Wang, Jingjing Jia, Maria Chikina, Hanna Pincas, Georgia Dolios, Kazuki Sasaki, Rong
Wang, Naoto Minamino, Stephen R. J. Salton and Stuart C. Sealfon. Characterization of gonadotrope
secretoproteome identifies neurosecretory protein VGF-derived peptide suppression of follicle-stimulating
hormone gene expression. Journal of Biological Chemistry 291:21322-34, 201611.

Takashi Tsuchiya, Tsukasa Osaki, Naoto Minamino, Kazuki Sasaki*. Peptidomics for studying limited proteolysis.
Journal of Proteome Research 14:4921-31, 2015

12.

Ji-Won Kim, Marie Rhee, Jae-Hyung Park, Hideki Yamaguchi, Kazuki Sasaki, Naoto Minamino, Masamitsu
Nakazato, Dae-Kyu Song, Kun-Ho Yoon. Chronic effects of neuroendocrine regulatory peptide (NERP-1 and -2)

on insulin secretion and gene expression in pancreatic β-cells. Biochemical and Biophysical ResearchCommunications 457:148-53, 2015
13.

Koji Toshinai, Takeshi Saito, Hideki Yamaguchi, Kazuki Sasaki, Wakaba Tsuchimochi, Naoto Minamino, Yoichi
Ueta, Masamitsu Nakazato. Neuroendocrine regulatory peptide-1 and -2 (NERPs) inhibit the excitability ofmagnocellular neurosecretory cells in the hypothalamus. Brain Research 1563:52-60, 201414.

Mio Nonaka, Ryang Kim, Hotaka Fukushima, Kazuki Sasaki, Kanzo Suzuki, Michiko Okamura, Yuichiro Ishii,
Takashi Kawashima, Satoshi Kamijo, Sayaka Takemoto-Kimura, Hiroyuki Okuno, Satoshi Kida, Haruhiko Bito.
Region-Specific Activation of CRTC1-CREB signaling mediates long-term fear memory. Neuron 84:92-106, 201415.

Arata Tomiyama, Takamasa Uekita, Reiko Kamata, Kazuki Sasaki, Junko Takita, Miki Ohira, Akira Nakagawara,
Chifumi Kitanaka, Kentaro Mori, Hideki Yamaguchi, Ryuichi Sakai. Flotillin-1 regulates oncogenic signaling inneuroblastoma cells by regulating ALK membrane association. Cancer Research 74:3790-801, 201416.

Kyohei Tokizane, Hiroyuki Konishi, Masaya Yasui, Tokiko Ogawa, Kazuki Sasaki, Naoto Minamino, Hiroshi
Kiyama. Continuous stress promotes expression of VGF in melanotroph via suppression of dopamine. Molecular
and Cellular Endocrinology 372:49-56, 201317.

Kazuki Sasaki*, Tsukasa Osaki, Naoto Minamino. Large-scale identification of endogenous secretory peptides
using electron transfer dissociation mass spectrometry. Molecular and Cellular Proteomics 12:700-9, 201318.

Shintaro Shimamura, Kazuki Sasaki, Masamitsu Tanaka. The Src substrate SKAP2 regulates actin assembly byinteracting with WAVE2 and cortactin proteins. Journal of Biological Chemistry 288:1171-83, 201319.

Abu Saleh Md. Moin, Hideki Yamaguchi, Marie Rhee, Ji-Wom Kim, Koji Toshinai, T. M. Zaved Waise, Farhana
Naznin, Takashi Matsuo, Kazuki Sasaki, Naoto Minamino, Kun-Ho Yoon, Masamitsu Nakazato. Neuroendocrine
regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro. Biochemical andBiophysical Research Communications 428:512-7, 201220.

Hiroaki Fujihara, Kazuki Sasaki, Emi Mishiro-Sato, Toyoaki Ohbuchi, Govindan Dayanithi, Motoo Yamasaki,
Yoichi Ueta, Naoto Minamino. Molecular characterization and biological function of neuroendocrine regulatory
peptide-3 in the rat. Endocrinology 153:1377-86, 201221.

Tsukasa Osaki, Kazuki Sasaki*, Naoto Minamino. Peptidomics-based discovery of an antimicrobial peptide
derived from insulin-like growth factor-binding protein 5. Journal of Proteome Research 10:1870-80, 2011

22.

Reiko Yagi, Masamitsu Tanaka, Kazuki Sasaki, Reiko Kamata, Yukihiro Nakanishi, Yae Kanai, Ryuichi Sakai.
ARAP3 inhibits peritoneal dissemination of scirrhous gastric carcinoma cells by regulating cell adhesion andinvasion. Oncogene 30:1413-21, 201123.

Kazuki Sasaki*, Noriyuki Takahashi, Mitsuo Satoh, Motoo Yamasaki, Naoto Minamino. A peptidomics strategy
for discovering endogenous bioactive peptides. Journal of Proteome Research 9:5047-52, 201024.

Koji Toshinai, Hideki Yamaguchi, Haruaki Kageyama, Takashi Matsuo, Keiichi Koshinaka, Kazuki Sasaki, Seiji
Shioda, Naoto Minamino, Masamitsu Nakazato. Neuroendocrine regulatory peptide-2 regulates feeding behavior
via the orexin system in the hypothalamus. American Journal of Physiology, Endocrinology and Metabolism
299:E394-401, 201025.

Takashi Matsuo, Hideki Yamaguchi, Haruaki Kageyama, Kazuki Sasaki, Seiji Shioda, Naoto Minamino,
Masamitsu Nakazato. Localization of neuroendocrine regulatory peptide-1 and-2 in human tissues. Regulatory
Peptides 163:43-8, 2010

26.

Emi Mishiro-Sato, Kazuki Sasaki, Takashi Matsuo, Haruaki Kageyama, Hideki Yamaguchi, Yukari Date, Masako
Matsubara, Takehiro Ishizu, Kumiko Yoshizawa-Kumagaye, Yoshinori Satomi, Toshifumi Takao, Seiji Shioda,
Masamitsu Nakazato, Naoto Minamino. Distribution of neuroendocrine regulatory peptide-1 and -2, andproteolytic processing of their precursor VGF protein in the rat. Journal of Neurochemistry 114:1097-106, 201027.

Aiko Tominaga, Hideki Sugawara, Toshitaka Futagawa, Kazuhiko Inoue, Kazuki Sasaki, Naoto Minamino,
Mamoru Hatakeyama, Hiroshi Handa, Atsuro Miyata. Characterization of the testis-specific promoter region in thehuman pituitary adenylate cyclase-activating polypeptide (PACAP) gene. Genes to Cells 15:595-606, 201028.

Kazuki Sasaki*, Yoshinori Satomi, Toshifumi Takao, Naoto Minamino. Snapshot peptidomics of the regulated
secretory pathway. Molecular and Cellular Proteomics 8:1638-47, 2009

29.

Masamitsu Tanaka, Kazuki Sasaki, Reiko Kamata, Yukari Hoshino, Kazuyoshi Yanagihara, Ryuichi Sakai. A novel
RNA-binding protein, Ossa/C9orf10, regulates activity of Src kinases to protect cells from oxidative stressinduced apoptosis. Molecular and Cellular Biology 29:402-13, 200930.

Tetsuo Ito, Noboru Maki, Osamu Hazeki, Kazuki Sasaki, Munenori Nekooki. Extracellular and transmembraneregion of a podocalyxin-like protein 1 fragment identified from colon cancer cell lines. Cell Biology International
31:1518-24, 2007

31.

Hideki Yamaguchi, Kazuki Sasaki (first author equal contribution), Yoshinori Satomi, Takuya Shimbara, Haruaki
Kageyama, Mondal MS, Koji Toshinai, Yukari Date, Luis J. González, Seiji Shioda, Toshifumi Takao, Masamitsu
Nakazato, Naoto Minamino. Peptidomic identification and biological validation of neuroendocrine regulatory
peptide-1 and -2. Journal of Biological Chemistry 282:26354-60, 2007

32.

Masamitsu Tanaka, Kazuki Sasaki, Reiko Kamata, Ryuichi Sakai. The C-terminus of ephrin-B1 regulates
metalloproteinase secretion and invasion of cancer cells. Journal of Cell Science 120:2179-89, 2007

33.

Tadaomi Naka, Etsuko Katsumata, Kazuki Sasaki, Naoto Minamino, Motoi Yoshioka, Yoshio Takei. Natriuretic
peptides in cetaceans: identification, molecular characterization and changes in plasma concentration after landing.
Zoological Science 24:577-87, 200734.

Sayaka Nagata, Johji Kato, Kazuki Sasaki, Naoto Minamino, Tanenao Eto, Kazuo Kitamura. Isolation andidentification of proangiotensin-12, a possible component of the renin-angiotensin system. Biochemical andBiophysical Research Communications 350:1026-31, 200635.

Yuko Yamaguchi, Mineo Kurokawa, Yoichi Imai, Koji Izutsu, Takashi Asai, Motoshi Ichikawa, Go Yamamoto,
Eriko Nitta, Tetsuya Yamagata, Kazuki Sasaki, Kinuko Mitani, Seishi Ogawa, Shigeru Chiba, Hisamaru Hirai.
AML1 is functionally regulated through p300-mediated acetylation on specific lysine residues. Journal of
Biological Chemistry 279:15630-8, 2004

36.

Kazuki Sasaki*, Kae Sato, Yasuto Akiyama, Kazuyoshi Yanagihara, Masaaki Oka, Ken Yamaguchi. Peptidomicsbased approach reveals the secretion of the 29-residue COOH-terminal fragment of the putative tumor suppressor
protein DMBT1 from pancreatic adenocarcinoma cell lines. Cancer Research 62:4894-8, 2002

37.

Yasuto Akiyama, Kouji Maruyama, Tohru Mochizuki, Kazuki Sasaki, Yoichi Takaue, Ken Yamaguchi.
Identification of HLA-A24-restricted CTL epitope encoded by the matrix protein pp65 of human cytomegalovirus.
Immunology Letters 83:21-30, 2002

38.

Kouji Maruyama, Yasuto Akiyama, Jinyan Cheng, Noriko Nara-Ashizawa, Takashi Hojo, Kazuki Sasaki, Ken
Yamaguchi. Hamster DEC-205, its primary structure, tissue and cellular distribution. Cancer Letters 181:223-32,

2002
39.

Kae Sato, Kazuki Sasaki*, Ming-Sound Tsao, Ken Yamaguchi. Peptide differential display of serum-free
conditioned medium from cancer cell lines. Cancer Letters 176:199-203, 2002

40.

Takao Ohkubo, Yasuhiko Sugawara, Kazuki Sasaki, Koji Maruyama, Naganari Ohkura, Masatoshi Makuuchi.
Early induction of nerve growth factor-induced genes after liver Resection-reperfusion injury. Journal of
Hepatology 36:210-7, 200241.

Kae Sato, Kazuki Sasaki*, Yasuto Akiyama, Ken Yamaguchi. Mass spectrometric high-throughput analysis ofserum-free conditioned medium from cancer cell lines. Cancer Letters 170:153-9, 2001

42.

Kouji Maruyama, Toshihiko Tsukada, Munehiro Honda, Noriko Nara-Ashizawa, Kiyoteru Noguchi, Jinyan Cheng,
Naganari Ohkura, Kazuki Sasaki, Ken Yamaguchi. Complementary DNA structure and genomic organization of
Drosophila menin. Molecular and Cellular Endocrinology 168:135-40, 2000

43.

Takao Ohkubo, Naganari Ohkura, Kouji Maruyama, Kazuki Sasaki, Koichi Nagasaki, Hiroaki Hanzawa,
Toshihiko Tsukada, Ken Yamaguchi. Early induction of the orphan nuclear receptor NOR-1 during cell death of
the human breast cancer cell line MCF-7. Molecular and Cellular Endocrinology 162:151-6, 2000

44.

Koichi Nagasaki, Kazuki Sasaki, Nicolai Maass, Toshihiko Tsukada, Hiroaki Hanzawa, Ken Yamaguchi.
Staurosporine enhances cAMP-induced expression of neural-specific gene VGF and tyrosine hydroxylase.
Neuroscience Letters 267:177-80, 1999

45.

Naganari Ohkura, Mikiko Ito, Toshihiko Tsukada, Kazuki Sasaki, Ken Yamaguchi, Keizaburo Miki. Alternative
splicing generates isoforms of human neuron-derived orphan receptor-1 (NOR-1) mRNA. Gene 211:79-85, 1998

46.

Kazuki Sasaki*, Kouji Maruyama, Eiji Nishimura, Toshihiko Tsukada, Ken Yamaguchi. Differentiation of cultured
neuroblastoma induced by staurosporine and cyclic AMP: methods for assessing a neuronal phenotype. Brain
Research Brain Research Protocols 1:399-405, 1997

47.

Kouji Maruyama, Toshihiko Tsukada, Shuji Bandoh, Kazuki Sasaki, Naganari Ohkura, Ken Yamaguchi. Retinoic
acids differentially regulate NOR-1 and its closely related orphan nuclear receptor genes in breast cancer cell line
MCF-7. Biochemical and Biophysical Research Communications 231:417-20, 1997

48.

Kouji Maruyama, Toshihiko Tsukada, Shuji Bandoh, Kazuki Sasaki, Naganari Ohkura, Ken Yamaguchi.
Expression of the putative transcription factor NOR-1 in the nervous, the endocrine and the immune systems andthe developing brain of the rat. Neuroendocrinology 65:2-8, 1997

49.

Naganari Ohkura, Mikiko Ito, Toshihiko Tsukada, Kazuki Sasaki, Ken Yamaguchi, Keizaburo Miki. Structure,
mapping and expression of a human NOR-1 gene, the third member of the Nur77/NGFI-B family. Biochimica etBiophysica Acta 1308:205-14, 1996

50.

Kazuki Sasaki*, Toshihiko Tsukada, Kouji Maruyama, Ken Yamaguchi. Long-term regulation of synapsin I gene
expression and neuronal morphology by cyclic AMP and low-dose staurosporine. Molecular Brain Research
40:157-60, 1996

51.

Eiji Nishimura, Kazuki Sasaki*, Kouji Maruyama, Toshihiko Tsukada, Ken Yamaguchi. Decrease in neuronRestrictive silencer factor (NRSF) mRNA levels during differentiation of cultured neuroblastoma cells.
Neuroscience Letters 211:101-4, 1996

52.

Naoko Kajimura, Hideaki Iseki, Rie Tanaka, Chiharu Ohue, Kotomi Otsubo, Motomichi Gyoutoku, Kazuki

Sasaki, Yasuto Akiyama, Ken Yamaguchi. Toxohormones Responsible for cancer cachexia syndrome in nude micebearing human cancer cell lines. Cancer Chemotherapy and Pharmacology 38 Suppl:S48-52, 1996,
53.

Kazuki Sasaki*, Toshihiko Tsukada, Isamu Adachi, Ken Yamaguchi. Staurosporine potentiates cAMP-mediatedpromoter activity of the vasoactive intestinal polypeptide gene in rat pheochromocytoma PC12 cells. Biochemical
and Biophysical Research Communications 214:1114-20, 1995

54.

Kouji Maruyama, Toshihiko Tsukada, Shuji Bandoh, Kazuki Sasaki, Naganari Ohkura, Ken Yamaguchi.
Expression of NOR-1 and its closely related members of the steroid/thyroid hormone receptor superfamily inhuman neuroblastoma cell lines. Cancer Letters 96:117-22, 1995

55.

Kazuki Sasaki*, Isamu Adachi, Noboru Yanaihara, Ken Yamaguchi. Neuronal cell differentiation of humanneuroblastoma NB-OK-I cells by staurosporine plus pituitary adenylate cyclase-activating peptide-38. Biomedical
Research 15:247-52, 1994

招待講演
1.

佐々木一樹：生理活性ペプチド探索のためのペプチドミクス

日本プロテオーム学会

2022 年 8 月 8日
2.

佐々木一樹：生理活性ペプチド探索への質量分析の応用- ペプチドミクス
ンポジウム

3.

京都大学薬学部

薬学の未来を考える京都シ2013 年 10 月 5 日

佐々木一樹：ペプチドミクスによる生理活性ペプチドの探索―実際と実例

第 15 回関東ハートセミナ

ー 2013 年 2 月 8 日
4.

佐々木一樹：日本生化学会シンポジウム「ペプチドミクスのための質量分析」

5.

佐々木一樹：日本内分泌学会

2011 年 9 月 22 日

内分泌代謝学サマーセミナー「質量分析を活用する分泌ペプチドーム解析」2010 年 7 月 9 日
6.

7.

Kazuki Sasaki: Role of ETD in peptidomics.

7th Annual Conference of Electron Capture andTransfer Dissociation2009 年 12 月 8 日

佐々木一樹、南野 直人:

日本生化学シンポジウム「分泌顆粒内ペプチドの解析から明らかになる世界」 2009 年 10 月 23 日
8.

佐々木一樹、南野 直人:

日本心血管内分泌代謝学会シンポジウム「ペプチドームからの新規生理活性ペプチド探索：NERP の発見」2007 年 11 月 17 日
9.

佐々木一樹：日本キャピラリー電気泳動学会シンポジウム「生理活性ペプチド探索のためのペプチドミ
クス」2007 年 11 月 15 日

10.

佐々木一樹：日本質量分析学会関西談話会「高分解能質量分析計によるペプチドミクス」2007 年 10 月
22 日

11.

佐々木一樹、尾崎司、南野 直人：日本薬学会シンポジウム「生理活性ペプチド探索を支援するペプチドミクス」2006 年 3 月 30 日
12.

佐々木一樹：日本プロテオーム学会シンポジウム「ペプチド性腫瘍マーカー探索のためのペプチドミク
ス」2005 年 5 月 20 日

13.

佐々木一樹：日本癌学会シンポジウム「質量分析計を用いる腫瘍マーカー探索」2001 年 9 月 28 日