Antibody Preparation��l�cl���l ���i��d� ��e������i�����d�c� �i�e����l�cl���l ���i��d� ��e�����i��
Fee
Antigenicity Investigation ＋ Peptide (～20AA, 80%, KLH）*1 ＋ Immmunization （Two Rabbits）

Delivery¥270,000/ set

Antigenicity Investigation ＋ Peptide （～20AA, 80%, KLH）*1 ＋ Immmunization（Two Rabitts）＋ Affinity Purification¥370,000/ set

Immunization （One Rabbit）¥100,000

Immunization (Two Rabitts）¥170,000> 3 and halfmonths> 2 and halfmonth

*1 Antigen Peptide is synthesized to the amount necessary for antibody preparation.
pAdditional amounts of the peptides applies
to an additional fee.

���i��FeeDelivery

Affinity Purification < 10ml

¥120,0000/sample> 2 weeks

Protein A Purification < 10ml

¥85,0000/sample> 2 weeks

Additional Immunization （Max 2 immunizations)for One Rabbit¥¥12,000Please ask

Workflow�eli�e�� ���d�c�
・Antiserum・Purified Antibody（for Purification Order）
・Operation
Oti Report
Rt

Suppply of Antigen

Peptide Synthesis

Protein Preparation

5 immunizations by 2 weeks （0W、2W、4W、6W、8W）
3 Antiserum Samplings （0W、5W、7W）

��������i��
・In the case of no increase in antibody valency, actual
costs for this service should be payable. The duration ofimmunization will be postponed according to the requests.
The additional immunization fee should be ppayable
yeven inthe case.
・The delivery date may be changeable according to theoperation.
・The freight of the sample to BEX should be payable.

Sending Antiserum Samplings
Antibody titer Check (2 ELISA)

Evaluation

Collection of Whole Blood or Additional Immunization

�l�� �� ��de�Please email orr send the order form downloaded from BEX web pageE-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

・Antigen ( > 3mmg) chilled* The shipment isn’t necessary in the case of use of peptide synthesized in BEX orprotein prepare
ed in BEX.

II. Please send the sample and order form to us.

・Order form
Address : 〒1773-0004 4F 61-15 2-Chome Itabashi Itabashi-ku Tokyo
BEX CO.,, LTD.
TEL: +81-3-5375-1071

BEX CO.,LTD.

http://www.bexnet.co.jp

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

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Antibody Preparation���������� �������� ������������（�������-����������）・In the system of “Quality-Guaranteed” the customers are free froom the risk unique to the biotechnological sample preparation:
Even if the preparation is failed, the money will never come again. Therefore,T
we provide Quality-Guaranteed service of monoclonal
antibody preparation. If the clone providing antibodies isn’t obtaineed, the fee isn’t payable. The condition of the “free” is that no
positive well is observed in the ELISA screening.
・Keeping mice in good condition is the key to prepare a good antibody.
ody We apply the method to lose the damage to mice in theimmunization operation and make two immunizations and final booster. Moreover, the systemized schedule from immunization toestablishing hybridoma can give you both of the low price and accuurate delivery.

������� ���������������� �������� �����������Price�elivery¥9000,000/Sample> 3 months

Price�eliveryAffinity Purification （ < 10ml ）¥1220,000/ Sample> 2 weeks

Protein A Purification（ < 10ml）¥85,000/ Sample> 2 weeks

Ascites Preparation¥200,000/ 1 animal> 1 month

Additional Clone¥35,000/ 1 clone

Please ask

Mainly mice: immunization to hybridoma establishment
Max 10 clones* Balb/c for the mouse or Wister line for the rat is used if no requests.

������Flow of Operatio
on
1, Antigen

Please get > 1mg of sam
mple ready.
Please ask us if you nee
ed to use a little mount of the antigen

2,Immunization

Two immunizations and final booster to Mouse
MAX in the case of protein antigen
First selection of Titer-M
Adjuvant may not be useed in the case of bacteria and cells

3,Cell Fusion

Cell fusion by PEG method with Mouse myeloma P3U1 is carried out individually.
Lymphocyte Cells are obbtained from spleen and lymph node.
PEG1500 is used for cell fusion.

4, Screening

ELISA is used for the anntibody screening. The plate combined with bacteria
and/or cells is acceptable. The antibody to immunized antigen is selected.

Free if Step 4 isn
isn’tt reached for the .Quality-G
Quality Guaranteed monoclonal preparation
5,Sample �elivery

BEX C�.,LT�.

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The supernatants of thee selected clones cultured in the 24-well plate isdelivered. Please evaluaate the samples.

�,Cloning

Selected clones are cloned. （Max 10 clones）Two clonings are usuallyy conducted by limiting dilution method.

�,Clone Establishment
Frozen Ampule Preparation

EstablishedEbli h d hybridoma
h b ida proliferated.
arelifd > 3 frozen
ftubesb forf eachhclone are prepared.
Subclass will be measured by the kit

http://www.bexnet.co.jp

e-mail info�bexnnet.co.jp

phone �81-3-53�5-10�1fax �81-3-53�5-5�3�Antibody Preparation�e���er� �ro����・>= 3 Frozen hybridoma vials for one clone (3～5×106 cells / vial）
・Cultured supernatant.
・Subclass data�n�or����on
・The quality-guaranteed may not be acceptable if the preparation is extremely difficult.
・If the preparation is not applied to the quality-guaranteed, the actuual fee should be payable in the unavoidable termination
during the operation.
・The delivery date will be changeable according to the operation.
・The freight of the sample to BEX should be payable.

F�o� �o �r�er
Please email orr send the order form downloaded from BEX web pageE-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

・Antigen ( > 1mg)
1Frozen* The shipment isn’t necessary in the case of use of peptide synthesized in
BEX or proteinn prepared in BEX.
II Please send the sample and order form to us.
II.
us・Order form
Address : 〒1773-0004 4F 61-15 2-Chome Itabashi Itabashi-ku Tokyo BEX
CO., LTD. TEL
L: +81-3-5375-1071

BEX CO.,LTD.

http://www.bexnet.co.jp

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

14