Cell-Related Service�ro�e�n ���re���on �� ���������n �e���Transfection to Mammalian cells, and preparation and purification ofo the targeted protein.
Amino acid substitution, defective mutant, etc are acceptable to esstablish the expression system. Please ask.

�ro���� ��ne��
Price

DeliveryConstruction of the vector *1¥80,000～/Sample> 2 weeks

Expression Analysis *2¥150,000/ Sample> 1 month

Small-Amounted Culture（100mL)・Purification Review *3¥200,000/ Sample> 1 month

Large-Amounted Culture (1L)・Purification *4¥400,000/ Sample> 2 month*1 The vector to add His-Tag or GST-Tag is used. Any other tag is accepptable. Please ask.
The price and delivery date to construct the Expressing vector dependds on the operation. Please refer to “subcloning” and/or “cDNA cloning.”*2 SDS-PAGE and Western Blotting is used to check the expression afterr small scaled transfection.
*3 The review may cause no acceptability for the large-amounted culture and purification afterwards.
*4 Affinity purification is used. Dialysis and/or gel filtration are acceptablee (resulting in additional fees). The condition for the expression should be
provided in advance in the case of the order only of large-amounted culturre and purification.

Flow of Operation�e���er� �ro����・Purified Protein
・Data of Expression Analysis
・Operation Reports

1,Establish the Vector
Preparation of the targeted gene from your vector and/or PCR
Ppproduction.
Check of insertion sequence.

�n�or����on

2,Expression Analysis

・The yield depends on the expressed protein.
・If necessary, NDA is agreeable. The operation is conductedunder the NDA
・The actual fee is payable in the unavoidable termination
after the discussion with the customers.
・ The deliveryy date depends
pon the operation.
p・The freight of the sample to BEX is payable.

The vecotr is transfected to the targeted cellTSSDS-PAGE
and Western Blotting is used to check the expression.

3,Small-Amounted Culture・Purification ReviewYYield
and purity check after expression and purification (100mL)

4,Large-Amounted Culture・Purification
Large-amounted culture under the condition decided above.
LPPurification
by Tag-specific affinity columnsF�o� �o �r�er
Please email orr send the order form downloaded from BEX web pageE-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

・Vector 2μg( > 100ng/μl） / cDNA（ > 1μg) / Cell （5×106 cells） /Frozen
Tissue （aboutt 10mg） / Total RNA（ > 10μg） / etc.
・Order Form
II. Please send the sample and order form to us.
Address: 〒1733-0004 4F 61-15 2-Chome Itabashi-ku Itabashi, Tokyo
BEX CO., LTD.
TEL: +81-3-5375-1071+BEX CO.,LTD.

http://www.bexnet.co.jp

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636

16

Cell-Related Service�ta��e Transfectant Esta��ishh�ent
Mammalian cells (CHO, COS, HEK293), or any other cell lines are trransfected, and the stable transfectant is established.

�rod�ct �ine���t �� Transfectant
�ta��eTf t t Esta��ish�ent
E t ��i ht

Stable Transfectant Establishment

PriceDei�ivery¥1,000,000/ Sample> 3 months* Expression analysis is conducted by Real-Time PCR or Westernblotting. Please choose one.
* The price and delivery date to constuct the Expressing vector depends ono the operation. Please refer to “subcloning” and/or “cDNA cloning.”* Special expression can be acceptable. Please ask.

��tionPriceDeivery

Additional Expression Analysis (Real-Time PCR or WesternBlotting)¥100,000/sample> 2 weeks

Western Blotting by Membrane fraction¥200,000/ sample> 2 weeks

Flow of OperationDe�i�ery �rod�ct
・DMSO Stock Cell Line （～1×106cells）
・Data of Expression Analysis
・Operation Report

1,Transfection
Transfection by Lipofection. The transfection condition is set in theTccaseof the cell lines with no performance of transfection.

2,Drug Screening

�nfor�ation
・There is no guarantee for the number of the clone sincesome clones drop out the targeted gene.
・If necessary, NDA is agreeable. The operation isconducted under the NDA
・The actual fee is payable in the unavoidable termination
after the discussion with the customers.
・The delivery date depends on the operation.
・The freight of the sample to BEX is payable.

Selection of the cells transfected with the vector by drug screening
SC
Checkof the drug sensitivity for the cell with no performance.

3,Cloning
C
Cloning
to establish max 10 clones by limiting dilution method

4,Expression Analysis
Expression Analysis by Real-Time PCR or Western Blotting
ET customer checks the result and pick up the clone.
TheF�o� to �rder
Please email orr send the order form downloaded from BEX web pageE-mail : info@bbexnet.co.jp
FAX : +81-3-5375-5636

I. Please send your requests to us.

・Cell Line （ > 1 Frozen Vial(s)）
・Vector 2μg ( > 100ng/μl）
・Order form

Frozen & Dry iced
Frozen & Dry iced

II. Please send the sample and order form to us.
Address: 〒1733-0004 4F 61-15 2-Chome Itabashi-ku Itabashi, Tokyo
BEX CO., LTD.
TEL: +81-3-5375-1071++813 5375 1071

BEX CO.,LTD.

17

http://www.bexnet.co.jp

e-mail info@bexnnet.co.jp

phone +81-3-5375-1071

fax +81-3-5375-5636