The Selectivity Company

YMC HT
Glass Columns
High Temperature Glass Columns
for laboratory scale
High Temperature
Applications
Easy Handlingand Operation
Self-packing

www.ymc.eu

HT2

HTContents

Product Range...................................................................................................................................................................................................................... 4
YMC HT Glass Columns.............................................................................................................................................................................................. 5
YMC HT Glass Columns – Your Benefits..................................................................................................................................................... 6
Engineered for Temperature Sensitive Applications..................................................................................................................... 7
Easy Handling and Column Packing............................................................................................................................................................... 8
Packing Adapter.............................................................................................................................................................................................................. 8
Full Resin Compatibility.............................................................................................................................................................................................. 9
Chemical Resistance.................................................................................................................................................................................................. 10
Easy Maintenance......................................................................................................................................................................................................... 11
Specifications....................................................................................................................................................................................................................12
Ordering Guide.................................................................................................................................................................................................................. 13
Column.................................................................................................................................................................................................................................13
Packing Adapter............................................................................................................................................................................................................ 14
Accessories and Spare Parts.............................................................................................................................................................................. 15
Training and Seminars............................................................................................................................................................................................... 16Laboratory Services..................................................................................................................................................................................................... 17More about YMC..............................................................................................................................................................................................................18
Questionnaire for YMC Glass Columns.................................................................................................................................................... 193

Product Range

HTProduct Range
Inner Diameter [mm]10, 15, 25, 50

Length Glass Body [mm]120, 200, 300, 450, 750, 1000

Temperature Range [°C]

4–70

Pressure Range* [bar]30–10* Pressure limit depends on inner diameter

4Wetted Parts

PEEK pistons, glass frits, FFKM O-ring,borosilicate glass bodyConnections

The columns are supplied with different adapters
to allow direct connection to any LC-system(e.g. ÄKTA system)

Accessories Supplied(columns with ID 10–15 mm)

1 x frit removal tool
2 x plugs, PTFE (1/4"-28G)
1 x 2 m FEP tubing (0.8 x 1.6 mm)
4 x 1/4"-28G nut and ferrule for 1/16" tubing
2 x M6 nut and ferrule for 1/16" tubing
2 x 10-32 fitting for 1/16" tubing

Accessories Supplied(columns with ID 25–50 mm)

1 x frit removal tool
2 x plugs, PTFE (1/4"-28G)
1 x 2 m FEP tubing (1.6 x 3.2 mm)
4 x 1/4"-28G nut and ferrule for 1/8" tubing
2 x M6 nut and ferrule for 1/8" tubing

YMC HT Glass ColumnsHTYMC HT Glass Columns
YMC HT glass columns are the perfect match forhigh temperature applications. The new column
line has been especially developed for conditionsof up to 70 °C. This offers more flexibility in yourmethod development. Due to the innovative column
design aqueous buffers and organic solvents canbe used. Easy handling and self-packing make theYMC HT glass columns your ideal partner forelevated temperature-sensitive applications.

• First choice for applications atelevated temperatures up to 70 °C• Pressure limit 30–10 bar dependingon the inner diameter• Practical column stands available forcolumns with 25 mm and 50 mm ID5HTYMC HT Glass Columns – Your Benefits

YMC HT Glass Columns – Your Benefits
Engineered for
Temperature-Sensitive Applications• Wide temperature range from 4–70 °C• Long lasting column design• Reliable connection to the heating jacket

Easy Handling and Column Packing• Optimum flow distribution• Reliable sealing• Reproducible packing results

Full Resin Compatibility• For all types of silica and soft gels
• Metal free flow path for BioLC applications• Resistant towards organic solvents

Easy Maintenance• Fast replacement wetted parts(e. g. frits and O-rings)• Efficient cleaning due to highsolvent resistance6

Engineered for Temperature Sensitive Applications

HT

Engineered for Temperature Sensitive Applications
Due to the high temperature stability and innovative design, temperatures of 4–70 °C can be used evenfor continuous operation. The robust column hardware makes it possible.

Borosilicate glass 3.3 heating jacket provides full organic solvent compatibility for efficient cleaning.
This can drastically extend the column lifetime.

7HT

Easy Handling and Column Packing / Packing Adapter

Easy Handling and Column Packing
The fully porous frits, which are securely fixed in thepiston, lead to optimal flow distribution. In combination with the linear piston movement, excellent
packing results are achieved. Even at temperaturesof 70 °C, reliable sealing due to the squeezed O-ringis guaranteed.

Packing Adapter
Packing adapters are used if the packing method requires additional column volume during the packing
process. For the slurry method a suspension of approximately 30–50 % packing material is prepared andtherefore often a higher column volume required. The adapter is coupled to the column an gives the higher
volume for packing. The additional packing solvent and packing adapter above the finished column bed caneasily be removed after the packing process.
1. Coupling unit
2. Snap ring
3. Spring washer
4. Counter screw
5. Glass bodyCompletely compwith ECO ad atible
apter
12
34

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Full Resin Compatibility / Available Resin from YMCFull Resin Compatibility
The YMC HT columns are produced exclusively from highly robust materials. Still, the flow path remains
metal-free. This allows the versatile use of YMC HT columns for all common LC modes such as NP, RP, affinity,
IEX and HIC.

ModeYMC HTIEX
SEC
HIC
Affinity
MMC
RPNP

Available Resin from YMC
Overview Resins for preparative Bioseparations

9HT

Chemical ResistanceChemical Resistance
Solvent

Wetted parts:
Borosilicate glass 3.3, PEEK,FFKM, FEP and glass frits

AcetoneAcetonitrile
Ammonium dihydrogen phosphate
Cyclohexane
Dichlormethane
EDTA (3 %)Acetic acid (6 %)Ethanol
Ethyl acetaten-hexane
Isopropanol
Methanol
NaOH (2 M)
HCl (1 M)
Urea (8 M)
NaCl (1 M)

Limited resistant

Na2SO4 (0.5 M)

Resistant

If your solvent is not listed, please contact us for a chemical resistance check.

Note: The resistance of the standard versions is based on the resistance ofeach wetted part. If one part is limited or not resistant, the whole column ismarked with this characteristic as this part is critical.
For all columns, where the combination of the wetted parts varies from the above
listed ones, the chemical resistance can vary, too. Therefore, please contact us
if you need assistance regarding the chemical resistance.

10

Easy Maintenance

HTEasy Maintenance
Quick and easy maintenance: The simple construction of the end piece makes it possible to replace
spare parts such as O-rings and frits quickly and easily.
1. Frit
2. O-ring
3. External piston
4. Inner piston
5. Counter screw
6. Spring washer
7. Snap ring
8. Counter nut
9. Screw nut

9382
4
1765

For ordering accessories or spare parts, please contact YMC!
11HTSpecifications

Specifications
Inner diameter column[mm]10152550

12

Pressure limit
[bar]

30

25

15

10

Bed length range[mm]

Column volume range[mL]

15–120

1.5–9

95–200

9–15

195–300

17–23

345–450

30–35

645–750

55–58

905–1000

77–78

10–120

2–19

90–200

18–32

190–300

37–49

335–450

65–73

635–750

121–122885–1000

163–170

10–120

6–60

90–20055–100

195–300

113–156

345–450

200–230

645–750

375–390895–1000

515–520

20–12045–235100–200

225–390

205–300

453–593355–450

785–885655–750

1450–1480

905–10001970–2000

HT

Ordering Guide Column

Ordering GuideColumn (Glass body and TWO adjustable end pieces)
The part numbers for the columns contain information on the column type, inner diameter, maximum bedlength and frits.
Inner diameter column[mm]

HT10HT15HT25Maximum bed length[mm]Product code

120

HT10/120G10200

HT10/200G10300
45010–16HT10/300G10
HT10/450G10

750

HT10/750G10

1000

HT10/999G10120

HT15/120G10200

HT15/200G10300
45010–16

HT15/300G10
HT15/450G10750

HT15/750G10

1000

HT15/999G10120

HT25/120G10200

HT25/200G10300
45010–16

HT25/300G10
HT25/450G10750

HT25/750G10

1000

HT25/999G10120

HT50/120G10200

HT50/200G10300
HT50Frit porosity[µm]

45010–16HT50/300G10HT50/450G10750

HT50/750G10

1000

HT50/999G1013HT

Ordering Guide Packing Adapter

Also findabout our gla outs s copacking serv lumnice.

Packing Adapter (Column coupler and glass body)Two different columns – one packing adapter:

The same packing adapters can be used for the new YMC HT as well as for the ECO line.

1. Select the correct column coupler
Inner
diameter column

Identification
for coupling unit

O-ring (FFKM)

ECO10 (10 mm ID)
ECO15 (15 mm ID)

K-3

KU/Combinations
ECO25 (25 mm ID)
ECO50 (50 mm ID)
ExampleECO10KU/K-3

2. Select the correct glass body
The inner diameters for the glass body must be the same as that of the YMC HT column.

Glass bodies with the following dimensions are available:
Inner Diameter [mm]Column length [mm]10, 15, 25, 50

120, 200, 450, 750, 1000

The length of the additional glass body must be selected according to the resin packaging instructions.
To select the correct length of the additional glass body, the following points can be used as a guide:•Double the volume of slurry compared to the required packed bed volume
if packing silica materials•Triple the volume of slurry for softer packing materials

The part number of the glass body is: ECO ID / length (e.g. ECO 10/120)

If you need assistance in choosing the right packing adapter, please contact YMC!

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Accessories and Spare Parts

HT

Accessories and Spare Parts
1. Column stand:For optimal positioning of the YMC HT glass column, column stands are available for IDs of 25 and 50 mm.
The reliable and handy design enables the column to be set up quickly and easily.

2. Packing Adapter:
Packing adapters are available for all available inner diameters in different lengths.
For more information see pages 8 and 14.

3. Spare Parts:
1. O-rings and frits
2. Glass body
3. Fittings and tubing/accessory packFor the complete listing of all available accessories and spare parts and their related ordering codes,
please request our pricelists as PDF files.

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HT

Training and Seminars

Training and Seminars
Glass Column Packing – Laboratory Scale and Pilot ColumnsT

his training will enable you to pack laboratory and process scale columns – using theory as well as practical experience. Different packing methods and procedures for preparing the packing process will
be discussed. In the hands-on part of the training, you will pack a column yourself in our applicationlaboratory. Helpful tips for troubleshooting and method optimisation will complement the seminar.

Online Seminar:
Theory of Glass Column Packing

Contents and Learning Objectives• How to achieve your packed column – packing methods you can use• Basics of preparation

Learn all strategies tosuccessfully pack a glass column• Hands-on experience – all steps you require for packing your column

Contents and learning objectives• Packing procedures: flow packing, axial compression and pack-in-place• Column qualification: determination of HETP and asymmetry using pulse test and transition analysis• Troubleshooting

Target Group
Glass column users with alternative qualifications (laboratory assistants, technicians,engineers, chemists).

Number of Attendees
4–8 people

Duration
2 hours

Date and Price• How you determine column efficiency• Troubleshooting – what you can learn from column
performance evaluation results

You can find actual dates and prices online athttps://ymc.eu/customer-training-and-education.html
Please contact us for further information and inquiries:
Dr. Mathias Hehn
Head of Laboratories
Phone: +49 2064 427-283
Fax: +49 2064 427-222
Email: hehn@ymc.eu

www.ymc.eu

Target group
Glass column users with alternative qualifications(laboratory assistants, technicians, engineers, chemists)
You can find actual dates for seminars in our seminar flyer
at www.ymc.eu or you can contact us directly:
Phone: +49 2064 427-0
Fax:+49 2064 427-222
Email: info@ymc.eu

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HTLaboratory Services

Laboratory Services
Save time and choose a packed column
If you are working on a tight schedule or you do not have sufficient experiencein column packing here is a solution!
Rely on the experience of YMC and ask for a packed YMC HT, ECO or ECOPLUS column.
Our knowledge of packing is your benefit!

Our services include packing of• YMC HT, ECO and ECOPLUS columns		 Depending on your required bed length, you can choose a column from our portfolio. If you can’t		 find a suitable standard product, we will gladly discuss the options of a customised column with you.
•						New glass columns and refill of your used YMC HT, ECO or ECOPLUS column
A second option for purchasing new column hardware is to use the option of our refill service if
you already have a YMC HT, ECO or ECOPLUS column. This service includes changing of frits anda quality control for the glass columns.

• Stationary phases from YMC as well as from other suppliers		 If your method is restricted to a certain stationary phase, we pack your column according		 to your needs, using stationary phases made by YMC or any other supplier.

How it works:Firstdiscussion ofrequirements

OfferColumnpackingColumn releaseaccordingto yourrequirements

Shipment
to yourlaboratory

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HT

More about YMCMore about YMC
YMC’s BioPro IEX are polymeric ion exchange
resins for the purification of biologicals.
They represent one part of the three part
YMC-Prep portfolio: silica, hybrid silica
and polymer media.

YMC-Triart Prep is a hybrid silica phase, stablebetween pH 2.0–10.0. It is available with various
modifications for RP purifications.

YMC PilotPLUS is the new standard for yourpilot scale applications. It is completely biocompatible
and characterised by safe and easy handling.
Packing, operating and unpacking the column
is a smooth procedure due to a numberof significant advantages.

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Questionnaire for YMC Glass Columns

For help in selecting your laboratory or pilot scale glass column, please fill in this questionnaire andsend a scan to info@ymc.eu.

Questionnaire for YMC Glass Columns
1. Inner diameter (mm):____________________________________________________________

2. Minimum bed length (mm):_______________________________________________________

3. Maximum bed length (mm):______________________________________________________

4. Bed volume (mL):_______________________________________________________________

5. Maximum pressure (bar): ________________________________________________________

6. Biocompatibility:

yesno

7. Packing material: _______________________________________________________________

8. Particle size of packing material: _________________________________________________

9. Solvents:_______________________________________________________________________

10. Operating temerature:___________________________________________________________

11. Quotation for packing adapter required:yesno

12. Regulatory requirements:________________________________________________________

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Your local distributor:Europe GmbHSchöttmannshof 19D-46539 Dinslaken
Germany
Phone +49 2064 427-0, Fax +49 2064 427-222
www.ymc.eu

Schweiz GmbHIm Wasenboden 84056 Basel
Switzerland
Phone +41 61561 80-50, Fax +41 61561 80-59
www.ymc-schweiz.ch

CO., LTD.

YMC Karasuma-Gojo Bld. 284 Daigo-cho,Karasuma Nishiiru Gojo-dori Shimogyo-ku,
Kyoto 600-8106 Japan
Phone +81 75342 45-15, Fax +81 75342 45-50
www.ymc.co.jp

YMC HT Glass Columns 04/2024_EN_V1.0