NEW
Bioinert Coated Columns

YMC Accura BioPro IEXOligonucleotides
Antibodies &Proteins
LC / MS Analyses

Highly accurate results
Exceptional recoveriesHigh throughput
Excellent reproducibility

Bioinert coated IEX columns

Non-porous YMC Accura BioPro IEXFeatures• Exceptionally high recoveries without preconditioning• Very sharp peak shapes with high sensitivities• Superior reproducibility and no carry-over effects• High efficiency and rapid throughput analyses• New rigid surface coated hardware

Ideal choice for• Oligonucleotides, nucleotides• Antibodies, proteins and peptides• Sensitive LC/MS analyses

Specification
YMC Accura BioPro IEX QF

YMC Accura BioPro IEX SF

hydrophilic polymer(polymethacrylate)hydrophilic polymer(polymethacrylate)Particle size / μm

3, 53, 5Pore size / nm

non-porous

non-porous

Charged group+-CH2N (CH3)3-(CH2)3SO3-Cl-Na+

2.0–12.0

2.0–12.0

MatrixCounter ion
Available pH range
Temperature range

4–60 °C

Pressure limit

3 µm: 15–20 MPa, 5 µm: 10–30 MPa

Column hardware

bioinert coated stainless steel

Frit hardware

HBioPro IEX
QFCH3CN

CH3H

CH3+strong anion
exchanger

BioPro IEXSFHCnHSO3–strong cationexchanger

Durable bioinert coating

Coating thickness: 400–1600 nm

The robust bioinert coating used on YMC Accura hardware is 130 to 320-foldthicker making it more durable than other similar hardware concepts. A longterm inertness against sensitive substances is ensured.
In order to demonstrate its robustness, a YMC Accura column was packed
multiple times. Even though this is quite a challenge for the column surface,
the coating remains unaffected (SEM* picture: top area is bare steel forcomparison).

*Scanning Electron Microscope

Page 2/8

Virtually No Adsorption on Oligonucleot

Guaranteed sensitivity and recovery
Sharp peaks and reliable recovery
for oligonucleotides
YMC-Accura
BioPro IEX QF

mV
20YMC Accura BioPro IEX QF

mV
2015151010n on Oligonucleotide
Analysis554th3rd2nd01st

Standard PEEK column
0mV
205101500min

Standard PEEK column

Column: 5 μm, 100 X 4.6 mmI.D.
Eluent: A) 20 mM Tris-HCl (pH 8.1)B) 20 mM Tris-HCl (pH 8.1) containing 1.0 M NaClO4
25-40%B (0-15 min), 40%B (15-20 min)Flow rate: 1.0 mL/min
Temperature: 60℃Detection: UV at 260 nm
Injection: 4 µL (5 nmol/mL)
Sample: 21mer RNA

1510Oligonucleotides ma
to their adsorption
recovery and peak
Accura BioPro IEX isAdsorption

4th

53rd
2nd
1st00in

5

1015minColumns:

YMC Accura BioPro IEX QF (5 µm) 100 x 4.6 mm ID (bioinert coated hardware)
BioPro IEX QF (5 µm) 100 x 4.6 mm ID (standard hardware)Part Nos.:
QF00S05-1046PTC
Oligonucleotides
may show poor peak shape/recovery on IEX analysis mainly due
QF00S05-1046WP
Eluent: to theirA) 20adsorption
mM Tris-HCl (pHonto8.1) the column hardware. Accura BioPro IEX gives goodB) 20 mM Tris-HCl (pH 8.1) containing 1.0 M NaClO4
recoveryandpeakshapefromGradient:25–40%B (0–15 min), 40%B (15–20min)the first injection. The results are reproducible.
Flow Accura
rate:
1.0mL/min
BioPro IEX is ideal for analysis of oligonucleotides. It does not require anyTemperature: 60 °C
preconditioning process before analysis.
Detection:
UV at 260 nm
Injection:
4 µL (5 nmol/mL)
Sample:21mer RNA
System:bioinert HPLC

Oligonucleotides generally exhibit poor peak shape and therefore low recovery in AEX analysis, mainly due toadsorption onto the column hardware. YMC Accura BioPro IEX columns provide high recovery and very goodpeak shapes from the first injection. This makes YMC Accura BioPro IEX QF columns ideal for the analysis ofoligonucleotides with reproducible results. The columns show stable peak areas from the 1st injection, so that nopreconditioning is required.
Page 3/8

Immediate reliability
No preconditioning required for reliable results

YMC Accura BioPro IEX SFmAU
300mAU300

000-50010102030

250

150

150

100

1005050

2013rd250 3

2nd2

1st200 1rdndmin

30stmin

2000-50150

0-500YMC Accura BioPro IEX SF (5 µm) 100 x 4.6 mm ID (bioinert coated hardware)
BioPro IEXthSF (5 µm) 100 x 4.6 mm 120%ID (standard 120%hardware)
100%Part Nos.:
SF00S05-1046PTC
100%
100
SF00S05-1046WP
rd80%80%
Eluent:A) 20 mM NaH2PO4-Na 2HPO4 (pH 6.8)
60%
60%containing 0.2 M NaClB) 20 mM NaH2PO4-Na 2HPO4 (pH 6.8)
50nd min), 0%B (30–45 min)Gradient:
0–50%B (0–3040%
40%
Flowrate:
0.5 mL/min
20%
20%
Temperature: 25 °C
st0%0%
Detection:
UV at 280 nm
0 50 252575 50
100 75Injection:
5 µL
Total load [µg] Total load [µg]Sample:
Bevacizumab (5 mg/mL)
System:bioinert UHPLCRecovery of Bevacizumab

43212030

1010202030

4th

4th3rd3rdnd

2nd
1ststmin30

Recovery of BevacizumabColumns:

20mAU300mAU 250
200300
4th

4th100100-50mAU
300

200200Standard PEEK column

min-500100

1020

Recovery of Bevacizumab

Higher recovery for low loading amounts
120%
100%80%60%
40%20%YMC Accura
PEEK hardware

0%255075100Total load [µg]The high inertness of YMC Accura BioPro IEX columns also requires no preconditioning in CEX analyses.
Especially at low loading amounts, YMC Accura BioPro IEX SF columns provide higher recoveries comparedto the standard PEEK column.
Page 4/8min

Column dimensions
according to analysis type
150 mm Length Column for High Resolution Analysis

Different column lengths for each separation purpose
2mV

Shortest
run time

80

50 x 4.6 mm ID

60

25–40%B (0–10 min)40

1Rs(1,2) = 3.2ColumnEluent

200010mV60

25–40%B (0–20 min)

140Rs(1,2) = 4.6

20001020Highest
resolution

80

260

25–40%B (0–30 min)

40Rs(1,2) = 5.1

120high resolution analyses.
Single-stranded RNA (antisense) and Doublestranded RNA are separated with Accura BioPro
IEX QF. As the column length becomes longer,resolution of two peaks becomes greater. Using alonger column can be one of the solutions toimprove resolution.

30 minmV

150 x 4.6 mm ID: Accura BioPro IEX QF (5 μm): A) 20 mM Tris-HCl (pH 8.1)
B) 20 mM Tris-HCl (pH 8.1) containing 1.0 M NaClO4: 0.5 mL/min: 60℃: UV at 260 nm: 8 µL (each 5 nmol/mL)

Bestise BioPro IEX has 150 mm length option forcompromAccura

280100 x 4.6 mm ID

Flow rate
Temperature
Detection
Injection

30 min20

1. antisense strand
2. siRNA duplex

00Columns:
Part Nos.:

Eluent:1020

YMC Accura BioPro IEX QF (5 µm)
QF00S05-0546PTC
QF00S05-1046PTC
QF00S05-1546PTC
A) 20 mM Tris-HCl (pH 8.1)
B) 20 mM Tris-HCl (pH 8.1) containing 1.0 M NaClO430 min

Flow rate:
0.5 mL/min
Temperature: 60 °C
Detection:
UV at 260 nm
Injection:
8 µL (each 5 nmol/mL)
System:bioinert HPLCYMC Accura BioPro IEX columns are available with different lengths for specific purposes. Short columns
provide short run times and high throughput, whilst retaining good resolution. Longer columns offer a higherresolution that is required for challenging samples, such as the separation of single- and double-stranded RNA.

Ideally suited for native IEX-MSmAU

1004030

a200Column:
Part No.:
Eluent1):

c10500bmAU

W0.5h (b'): 0.125
Rs(a'/b'): 2.50Rs(b'/c') : 1.51

01020

YMC Accura BioPro IEX SF (3 µm) 100 x 2.1 mm ID
SF00S03-10Q1PTC
A) 20 mM CH3COONH4-CH3COOH (pH 5.6)
B) 140 mM CH3COONH4-10 mM NH4HCO3 (pH 7.4)
Gradient: 20%B (0–2 min), 20–100%B (2–18 min), 100%B (18–22 min)Flow rate: 0.1 mL/min

14.0

15.0

16.0minmin

Temperature: 25 °C
Detection:
UV at 280 nm
Injection:
2 µL
Sample:Trastuzumab (1 mg/mL)
System:bioinert UHPLC			1) Anal. Chem. 2018, 90, 13013-13020.

Smaller column IDs that allow lower flow rates and volatile mobile phases are necessities for coupling tomass detection. Such high sensitivity analyses are ideally performed using YMC Accura BioPro IEX columns.
Another positive effect is the decreased solvent consumption and lower amount of sample required.
Page 5/8

Reproducibility and stability
Excellent batch-to-batch reproducibility

Ideal foruse inntrol !
quality co

Column lot E

Column lot D

Column lot C

Column lot B

Column lot A
05

1015

2025

Column:Part No.:
Eluent:30

BioPro IEX SF (5 μm) 100 x 4.6 mm ID
SF00S05-1046WPA) 20 mM NaH2PO4-Na 2HPO4 (pH 6.5)
B) 20 mM NaH2PO4-Na 2HPO4 (pH 6.5) containing 0.2 M NaClGradient: 0–50%B (0.5–30 min)min

Flow rate:
Temperature:
Detection:
Injection:
Sample:

0.5 mL/min
25 °C
UV at 215 nm
10 μL
Monoclonal antibody (IgG1)

BioPro IEX columns exhibit excellent batch-to-batch reproducibility – as in this example – for mAb analysis
with resolution of peaks for small charge variants. All gel batches are inspected by rigorous quality control
tests and must meet the required criteria before release.
BioPro IEX columns are the best choice for the quality control of mAbs, proteins, oligonucleotides andother biopharmaceuticals.

High temperature stabilitymAU45mAU

2113540353030252520120h at60ºC

201510151055000Column:
Part No.:
Eluent:510

BioPro IEX QF (5 µm) 100 x 4.6 mm ID
QF00S05-1046WPA) 10 mM NaOHB) 10 mM NaOH containing 1.0 M NaClO4Gradient: 25–55%B (0–15 min), 100%B (15–20 min)Flow rate: 1.0 ml/min

015 min

52Stableretention
times and sepeak shap
1015 minTemperature: 25 ºC
Detection:
UV at 260 nm
Injection:
4 µl (each 5 nmol/ml)
Sample:1) 5’-TCATCACACTGAATACCAAT-3’ (DNA 20mer)2) 5’-GTCATCACACTGAATACCAAT-3’ (DNA 21mer)

Page 6/8

Application examples

Application: Analysis of Plasmid (Topological Isomers)

Analysis of topological isomers of plasmids
ColumnEluent: Accura BioPro IEX QF (5 μm), 100 X 4.6 mmI.D.
: A) 20 mM Tris-HCl (pH 8.5)
B) 20 mM Tris-HCl (pH 8.5) containing 1.0 M NaCl
77%B (0-1 min), 77-85%B (1-15 min): 0.4 mL/min: 25℃: UV at 260 nm: 1 µL (50-65 μg/mL): pUC19 plasmid extracted from E. coli (2686 bp)
pUC19 plasmid digested with nicking endonuclease
pUC19 plasmid digested with restriction enzyme BacoiledrepusehEven thighhtiw
mrof
ciencyfife
noitctransfearated.
pesllew
isSupercoiled (SC)mAU

Flow rate
Temperature
Detection
Injection
Sample (SC)
(OC)
(L)

Open circular (OC)20Linear (L)15105Plasmid DNA is analyzed with Accura BioPro
QF. Supercoiled form, which has high transfe
efficiency, is well separated with other topolo
isomers.

00105minColumn:Part No.:
Eluent:YMC Accura BioPro IEX QF (5 μm) 100 x 4.6 mm ID
QF00S05-1046PTC
A) 20 mM Tris-HCl (pH 8.5)
B) 20 mM Tris-HCl (pH 8.5) containing 1.0 M NaCl
Gradient: 77%B (0–1 min), 77–85%B (1–15 min)Flow rate: 0.4 mL/min

Temperature: 25 °C
Detection:
UV at 260 nm
Injection:
1 µL (50–65 μg/mL)
Samples:
pUC19 plasmid extracted from E. coli (2686 bp) (SC)
pUC19 plasmid digested with nicking endonuclease Nt.BspQI (OC)
pUC19 plasmid digested with restriction enzyme BamHI (L)
System:bioinert UHPLCHigher resolution for monoclonal antibody analyses

lutionoserreW (b') : 0.081
tBet!
ht
di
Rs(a'/b') : 2.99
w
Rs(b'/c') : 1.27
and peak
mAU

mAU
x10

2x10

2.0
1.9
1.8
1.7
1.6
1.5
1.4
1.3

22.0

a b

W0.5(b) = 0.060
Rs(a,b) = 0.88
Rs(b,c) = 1.31

1.9
1.8

1.6

3.8

1.5

3.6

1.4

3.4

1.3

3.2
3.0

1.2

2.6

1.0

2.4
2.2

0.9

1.2

c2.8

1.1

1.1

YMC-Accura
BioPro IEX SF
YMC Accura BioPro IEX SF
100 x 4.6 mm ID
1.0
0.9

2.0

0.8

0.8

1.8

0.7

1.6

0.7

1.4

0.6

0.6

1.2

0.5

0.5

1.0
0.8

0.4

0.4

0.6

0.3

0.4

0.2

0.2
0.0

0.1

7.4

0.0
12

34

56

789

10111213

1415

7.6

7.8

8.0

8.2

8.4

8.6

8.8

9.0

9.29.4

9.6

9.8

10.0

10.2

10.4

100 x 4.6 mm ID

0.3
0.2
0.1
0.0

min

1mAU

1.8
1.7
1.6
1.5
1.4
1.3
1.2

23456mAU

2x10
2.0
1.9

0.5

1.7

x10 1

2x10
2.0bW0.5(b) = 0.121
Rs(a,b) = 0.41
Rs(b,c) = 0.70

1.9
1.8
1.7

x10 1

1.6

3.8

1.5

3.6
3.4

1.4

3.2

a

3.0
2.8

1.1

2.6
2.4

1.0

1.3
1.2
1.1

2.0
1.8

0.8

1.6

0.7

1.0c

2.2

0.9

0.9

1.4

0.6

1.2
1.00.5

0.8

0.4

0.6

0.3

0.4
0.2

0.2

0.0

0.1

8.2

8.4

8.6

8.8

9.0

9.2

9.4

9.6

9.8

10.0

W0.5(b') : 0.109
Rs(a'/b') : 2.58
Rs(b'/c') : 1.15

10.2

10.4

10.6

10.8

11.0

11.2

ProPac3R3RSCXProPacSCX
100x4.0mm
100 x 4.0 mm IDID

0.0

0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0

12

345

678

91011121314

Column: 		 YMC Accura BioPro IEX SF (3 µm) 100 x 4.6 mm ID
Part No.:		 SF00S03-1046PTCEluent:		 A) 10 mM MES*-NaOH (pH 6.6)		 B) 10 mM MES*-NaOH (pH 6.6) containing 1 M NaClGradient:		 0–20%B (0–15 min)Flow rate:		 0.5 mL/min for 4.6 mm ID, 0.378 mL/min for 4.0 mm ID

151min2Temperature: 25 °C
Detection:
UV at 280 nm
Injection:
2 µL for 4.6 mm ID, 1.5 µL for 4.0 mm ID
Sample:Bevacizumab (5 mg/mL)
System:bioinert UHPLCProPac is a trademark of Thermo Fisher Scientific Inc.

*2-(N-morpholino) ethanesulfonic acidPage 7/834567

Ordering information3 µm non-porous analytical columns, bioinert coated hardware (max. pressure 15–20 MPa)
Phase

Column ID(mm)

Column length(mm)50 (15 MPa)

100 (15 MPa)

150 (20 MPa)

YMC Accura BioPro IEX QF

2.1
4.6

QF00S03-05Q1PTC
QF00S03-0546PTC

QF00S03-10Q1PTC
QF00S03-1046PTC

QF00S03-15Q1PTC
QF00S03-1546PTC

YMC Accura BioPro IEX SF

2.1
4.6

SF00S03-05Q1PTC
SF00S03-0546PTC

SF00S03-10Q1PTC
SF00S03-1046PTC

SF00S03-15Q1PTC
SF00S03-1546PTC

5 µm non-porous analytical columns, bioinert coated hardware (max. pressure 10–30 MPa)
Phase

Column ID(mm)

Column length(mm)50 (10 MPa)

100 (12 MPa)

150 (18 MPa)

250 (30 MPa)

YMC Accura BioPro IEX QF

2.1
4.6

QF00S05-05Q1PTC
QF00S05-0546PTC

QF00S05-10Q1PTC
QF00S05-1046PTC

QF00S05-15Q1PTC
QF00S05-1546PTC–QF00S05-2546PTC

YMC Accura BioPro IEX SF

2.1
4.6

SF00S05-05Q1PTC
SF00S05-0546PTC

SF00S05-10Q1PTC
SF00S05-1046PTC

SF00S05-15Q1PTC
SF00S05-1546PTC–SF00S05-2546PTCYMC CO., LTD.

https://www.ymc.co.jp/

YMC Europe GmbHhttps://ymc.eu/

YMC America, Inc.

https://www.ymcamerica.com/

YMC Switzerland LLC

http://www.ymc-schweiz.ch

YMC India Pvt. Ltd.
https://ymcindia.com/

YMC Shanghai Rep. Officehttps://www.ymcchina.com/

YMC Korea Co., Ltd.

https://www.ymckorea.com/

YMC Taiwan Co., Ltd.
https://ymctaiwan.com/

YMC Singapore Tradelinks Pte. Ltd.
https://ymc.sg/

Page 8/8