Core Shell Particle

Totally porous particle

Add C18 hydrophobicity to PFP separation!
Increased retention and improved durability
Comparison of separation
Fluorobenzene

Standard samples

Column dimension: 4.6 x 150 mm
Mobile phase: CH3OH/H2O=75/25
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = Uracil, 2 = Caffeine, 3 = Phenol, 4 = Butylbenzene
5 = o-Terphenyl, 6 = Amylbenzene, 7 = Triphenylene
Hydrogen bond

PFP
PFP&C18
C18(Caffeine/Phenol)

Hydrophobicity(Amylbenzene/Butylbenzene)(Triphenylene/o-Terphenyl)

Steric selectivity

1.00
1.00
0.39

1.31
1.47
1.60

2.38
2.64
1.46

Column: SunShell PFP 2.6 μm, 4.6 x 150 mm
SunShell PFP&C18 2.6 μm, 4.6 x 150 mm
SunShell C18 2.6 μm, 4.6 x 150 mm
Mobile phase: Methanol/water=60/40
Flow rate: 1.0 mL / minTemperature: 40 oC
Detection: UV@250 nm
Sample:1 = Benzene, 2 = Fluorobenzene, 3 = Toluene, 4 = α,α,α-Trifluorotoluene
FCH3FFF

Compared to PFP, PFP & C18 has the same stereoselectivity, but the hydrophobicity isgreatly increased and the retention time is extended. PFP & C18 has achieved thesame separation as that of PFP, such as the separation of fluorobenzenes.

Relative plate

Stability test
Conditions for durability testing
Column: Sunniest PFP&C18 5 μm, 2.1x150 mm
Sunniest PFP 5 μm, 2.1x150 mm
Mobil phase: CH3OH/20mM PB pH7.0=70/30
Flow rate: 0.2 mL/min
Temperature: 40 ºC

3 times

PFP＆C18PFP
Elution time/hConditions for measuring theoreticalplates
Column: PFP&C18 5 μm, 2.1x150 mm
PFP 5 μm, 2.1x150 mm
Mobil phase: Acetonitrile/water=70/30
Acetonitrile/water=60/40
Flow rate: 0.2 mL/min
Temperature: 40 ºC
Sample: Acenaphthene

Due to the increased hydrophobicity of the surface,
the durability of PFP & C18 is approximately threetimes that of conventional PFP.

ChromaNik Technologies Inc.

Comparison of retention times for highly polar compounds
２

1Column: Sunniest PFP&C18, PFP 5 μm, 150 x 4.6 mm
Mobile phase: 12.5 mM Ammonium Formate (pH3)
Flow rate: 1.0 mL/min
Temperature: 25 ºC
Detection: UV@260nm

Sunniest PFP＆C18２112Sunniest PFPOHOHNHOOHHONH2HOOH

Levodopa

Epinephrine

In the separation of highly polar compounds such as catecholamines, the selectivity ofPFP and PFP & C18 changes greatly, and PFP tends to be more retained.

◆ Specification of SunShell PFP&C18, Sunniest PFP&C18
Bonded phase

Core shell silica and totally porous silica
Pore size(μm)

Corediameter(μm)

Porediameter(nm)

SunShell PFP&C18

2.6 μm

1.6 μmSunniest PFP&C18

5 μm

0 μm

Specific surface
area (m2/g)

Carbonloading(%)

Stationary phase

USP Lline

End-capping

Maximumpressure

pH range

9 nm

150 m2/g

6%

Pentafluorophenyl + C18

L43

TMS end-capping

60 MPa2-8

12 nm

340 m2/g

14%

Pentafluorophenyl + C18

L43

TMS end-capping

30 MPa2-8※For the maximum operating pressure, the value described in the test report of each column has priority over the above value.

PFP&C18 stationary phase schematic diagramOO SiOOOO SiFFFFF◆ Ordering information

SunShell PFP&C18, 2.6 µm

Sunniest PFP&C18,
5 µm

Inner diameter (mm)2.1

Length (mm)305075100
150

Catalog No.
CV6931
CV6941CV6951CV6961
CV6971

Inner diameter (mm)2.1

Length (mm)50100150250

Catalog No.
EV3241
EV3261
EV3271
EV3281

3.04.61020

Catalog No. Catalog No. Catalog No. Catalog No.
CV6331CV6431----------CV6341
CV6441----------CV6351
CV6451----------CV6361
CV6461----------CV6371
CV6471-----------

3.0

4.61020

Catalog No. Catalog No. Catalog No. Catalog No.
EV3341
EV3441----------EV3361
EV3461----------EV3371
EV3471----------EV3381
EV3481
EV3781
EV3881

USP categoryL43

USP category

L43

Manufacturer

ChromaNik Technologies Inc.

1909

6-3-1 Namiyoke, Minato-ku, Osaka, 552-0001 Japan
TEL: +81-6-6581-0885 FAX: +81-6-6581-0890
E-mail: info@chromanik.co.jp
URL: http://chromanik.co.jp