M179-A48
Page 1For Research Use Only.
Not for use in diagnostic procedures.

Anti-GM130-Alexa Fluor® 488
CODE No.

M179-A48

CLONALITY
CLONEISOTYPE
QUANTITY

Monoclonal
5G8
Mouse IgG2a 100 L, 1 mg/mL

SOURCEIMMUNOGEN
FORMURATION

Purified IgG from hybridoma supernatant
Human GM130, C-terminal (synthetic peptide)
PBS containing 1% BSA and 0.09% NaN3.

*Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when
disposing materials containing azide into drain.

STORAGEThis antibody solution is stable for one year from the date of purchase when stored at 4°C.

APPLICATIONS-CONFIRMED
Immunocytochemistry

20 g/mL

SPECIES CROSS REACTIVITY on WB
Species

HumanMouse

Rat

Hamster

Cells

HeLa, 293T, A549

Not tested

Not tested

Not tested

Reactivity







Entrez Gene ID

2801 (Human)

REFERENCES

1) Diao, A., et al., J. Biol. Chem. 283, 6957-6967 (2008)
2) Alvarez, C., et al., J. Biol. Chem. 276, 2693-2700 (2001)

For more information, please visit our web site https://ruo.mbl.co.jp/LABEL LICENSES:This product is provided under an agreement between LIFE TECHNOLOGIES Corporation, and Medical & Biological Laboratories Co.,
LTD, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, andcorresponding non-U.S. equivalents, owned by or licensed to LIFE TECHNOLOGIES Corporation. For information on purchasing a license
to this product for any other use, contact LIFE TECHNOLOGIES Corporation, Molecular Probes Detection Technologies, BusinessDevelopment, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

MEDICAL & BIOLOGICAL LABORATORIES CO., LTD.
URL https://ruo.mbl.co.jp/
e-mail support@mbl.co.jp, TEL 052-238-1904

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WB: Western blotting
IP: Immunoprecipitation
IC: Immunocytochemistry
IHC: Immunohistochemistry
FCM: Flow cytometryM179-A48
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Immunocytochemistrye1) Spread the cells in the nutrient condition on a glass slide, then incubate in a CO2 incubator for one night.
2) Remove the culture supernatant2 by careful aspiration.
3) Fix the cells by immersing the slide in 4% paraformaldehyde (PFA)/PBS for 10 minutes at room temperature (20~25oC).
4) Prepare a wash container such as a 500 mL beaker with a magnetic stirrer. Then wash the fixed cells on the glass slide bysoaking the slide with a plenty of PBS in the wash container for 5 minutes. Take care not to touch the cells. Repeat anotherwash twice more.
5) Immerse the slide in 0.2% Triton X-100/PBS for 10 minutes at room temperature.
6) Wash the slide 2 times with PBS.
7) Add 200 L of the primary antibody diluted with 2% fetal calf serum (FCS)/PBS as suggested in the APPLICATIONS ontothe cells and incubate for 30 minutes at room temperature. (Optimization of antibody concentration or incubation condition isrecommended if necessary.)8) Wash the slide 2 times with PBS.
9) Counter stain with DAPI for 5 minutes at room temperature.
10) Wash the slide 2 times with PBS.
11) Wipe excess liquid from slide but take care not to touch the cells. Never leave the cells to dry.
12) Promptly add mounting medium onto the slide, then put a cover slip on it.
(Positive control for Immunocytechemistry; HeLa)

Immunocytochemical detection of GM130 in HeLa
Green: M179-A48
Blue: DAPI

110930-1