( // ¼ ,**/

89- 
Some Problems of Legionella in the Hot Spring Waters
Naoyuki KATO School of Medicine, Toho University

+32+    Saito et al., +32+  ! "# $%& '%( )*+

$%& 0$0 1230( *,+-.(/40506789 : =>?@ AB#0(. ;<  %(/"#$.
! %&C8DE FGHIJKLMN0(/ =#=#O$.PQ
23R0(/ /&  S23Q! %$ 23TUVJWXYZ 0"#[\,& Q ]^VJ!/WXY="0N_JK# %&VJ#$%P0 `$. / &* ,***  - abcdefEg23'!TU ,- h0 , h506,& = ,**,  2 a
ijekg( - lmno23TU ,3/ h0 1 h506)*,p+#0(qr 6# %&""23#

#s]-t/(uv(w&VJx#$ 2,Zy-.^ . z {|}no,/~o?o/08h1234$.56^ 78Z- /[\# 98ZW
Z:0WXZ;Q"#0VJ0(/& y<W=>0  WRQ?N8#%(@# & "WXY8AB Z#8AWX<x# ?N8ZB ?N898CZ'!0N8<x,- *8 Dl?mo}8EF
$G - C , 8A<xZ£!0(/W¡](/& ¢RVJx#

HB08Q/N8<x D¤¥o¦Z§¨?N8I©08QM

 ª«¬/#(/& =^W­8A<x®/(=¥o¦I©. N8
<xJ¯°± /#²]&""VJWX®³ , t Kt/(´¯&+& VJ0(/23Zµ¶$.L!µM·(/K,&NVJ¸µOQ"# %(/¹.(/ /23D/&,&ZºBQPBºB»Z¡!0(/¤N8<xZ-(/WXµW98ZCQ?N8Z=º0(0+/ NPB,N8 
(/ /K,&#$»¼90

  !"
#$%&'()

*+,-.

/0'1 23

4#$'*+,

567'(89 *+( :;<= >?@ A?@ B@ CDE
FG H#' *+I 19 JKL(MN 1:;*+,OPM:; Q, *.,R*.. ppm ST U VW +.* ppm XY!"Z[9 :;*+( pHH\]^ _`a9 b pH

H#$'(XYHQ :;,c.de f\ ghi(j'f!"'19 klm#$'( no:;l p]^ _`qr stu.vwx 19y #$' :;*+zZ{|}~(+9  .!]:; Q ,9 M:; Q  -9 :;! .9  I }9 yMN 1*+ >?@!A?@ 19 >?@*.*/ ppm 'pH |}3z*+]^,(s'Q[ 2 +8> ,& #$%&'( *.*- ppm9 ~( f

H, S, Cl , SO.

,Q' (|}I a }p Q a¡]N¢7I'19 *+ Q'(£¤z,o3 2¥¦§¨+3218

W© 'st ª«o}

9A?@*+'( pH,¬\­7!"'19 o® pH 1 '(]^ ¯q}pH 3 '(]^,[| °±²³´|} 2Lin et.al., ,**,89 #$,µ(56}9 kza¶· ¸!"'19 >?@¹!MN 1 + ©I " 1} ³´º

 L. pneumophila » // ¼ J,**/L2e+AB CD Mº91   !"#$%&'()*+ , -./0,+  12345(6789:  ;<= 1*>2*? +- AB CD EFGHI= JK ,L534@M NO+PQR STCUVG

WXYZ[\]^ 34_`aX<bc

+d^<2efg+hij 2e+ek k5lm2eABCD+5no(p  3dq7rs+t]u2e8,+vwu<=d"X =Wp  M AB CD+x5y= qz )*"{|+]u2e+ek5WX}qd~ , Acanthamoeba

 VNC ++}xXt b ( Viable But

Nonculturable JVNCL +( JMichael et al., +331L =q2eAB C

Dc+d$xX^5AB CDῌGD JLegionella pneumophilaL 5
 VNC +!5a"X =q2" />./? pH />3 +xb
 z 2" .*>./? pH />2  VNC ax^" +d L. pneumophila  2e+2" ./? ^ pH  / + xXt  Xu#5$I@Y z5 8+;<= #%+x !
&¡¢'£¤:5¥ JOhno et al., ,**-L+dAB CD2e¦+§ VNC x¨©+2e¦5ª«8Z¬G­®I= ^++AB CD¯<^+ °+(z-±²¬³GB+§ ´+[µ¶·=-5)AB CDz(¸5¹X

U¼92

 

pneumophila " #$%& '  ! L.

) * + 

(,-./0 (* 1) +,*** 23  45 6
 ,-./07$8 )9":;<6=>//0"?@A',-.=>/B0 # C95 5   D
L. pneumophila EFG H6 ! # C"I =>/B0'#J $%  5 +,-./0  GK L&MN

H'O (K) L&MN"*P H5  QRST= (U#+ V,,-./0-# -W XY.6
(L. pneumophila " /^_`,-R[\]Z

6A01"A* A-W5 6D 235 6QRST= 4aa;  b

`Bc 5dF)efgFRfhR 3 b`Bc #i5j +k/ mm l m%&;;/ b`Bc

" A no ) 68 6pq789 QRST=r59s + at: "uo )   !QRST= ;v5<t wx< (=>) QRST= *.,k*.. mgῌl =>y?t z@ * D A

" A  ;6B{|"<6CF}~D 3E"'FF)68 8#"7F 8#m5;; "m;  G (>S,c0*  $)  >S,c0QRST=H )  I Jtw) KLMNO#PQR) 8# (ST* <UE4VW6 L. pneumophila "35  ;6 X6 D  *.,k*.. mgῌl tYZ  " ) 68   ) "D<6 [5 6QRST="tYZ"\6A)-<3 ]"5 6 Di5: 6 ' t`#UabcdlD#;#^J_Jt Uef5d'<tg<hiJ "$5"H]j5 ) V?

U  k  5 ) Ulm¡ - am"n% ) ¢6 jU 7  KLMN9 £ ¤Eio ¤io ¥¦pD6pῌ§Dqr©< ! qr)

7¨'stª«\_ )¬ i# 'uv­ 5 )6   p®wD  Utux4C¯y wz"{$ +1 | , ° ,.
}±~5  / ° ,. }D u5) _­¯y f² . ³D" 5(p®w ,**/* ´¯y"<uo ) lµ| <)668¶U·¸ FU  ¶QRST=i5¸  ¹6D

 . ³D" 5 # <6)



7º"3 U) )¬ D»  A º // ¼ H,**/U#$|93

῍ ῌ ῎   ! " #$%&'(῎)*+, -./01 (234(56! "' (78/9:4 ;<=>?(@!" %AB 8/9:4/0CD;
;<(=>E 'B FG HIJ4%AB KL4 , MNOPLQRS? 5(T<D U V<;῍W῎)X*+YZ[\?
5(]^<D " _`DKLab῎)c8defgῒhCD :KL?

5(E" _4idefg:KL?

((FD+c8jk6lB mn:op? 5(q῍! " r ῎)5_stu(vwx6yz{Bmn|opB }~:& q῍! "a);</0CD 5(! " 5D)#8 +B defgIB g W[\4 5(" )#8

0* |5(( B 1* |5(:? "#$ 1 a:*4 " ῑ<` ¡?
s!+B _ῑ¢5(#$£¤¥s! " 4 V<;

KL:& 5(q῍!6 " 4;45D;<#$P ¦! ;23CD

5(;<B ῏ῌ§¨©ῐ4;#$;B C<KLabªs«¬ !6 "ῑῐῒ῏­® H,**/UB ¯°­ῌ¯°±²B #$¥(῏ῌ www.env.go.jp/nature/³´µ¶fB L.A. H+321U : ·¸¹B º 3 »"Lin,Yu-sen E., Radisav D. Vidic., Janet E.Stout. and Victor L. Yu (,**,) : Negative e#ect ofhigh pH on biocidal e$cacy of copper and silver ions in controlling Legionella
pneumophila. Appl Environ Microbiol., 02, ,1++ῌ,1+/.
Michael, S., Levente, E., Rudolf, A. and Jörg, H. (+331) : Resuscitation of Viable but Nonculturable Legionella Pneumophila Philadelphia JR-, by Acanthamoeba castellanii. Appl Environ Microbiol., 0-, ,*.1ῌ,*/-.
Ohno, A., Kato, N., Yamada, K. and Yamaguchi, K. (,**-) : Factors inﬂuencing of Legionella
pneumophila serotype + in hot spring water and tap water. Appl Environ Microbiol., 03,,/.*ῌ,/.1.
Saito, A., Shimada, T., Nagasawa, M., Tanaka, H., Ito, N., Shigeno, Y., Yamaguchi, K., Nakatomi,M. and Hara, K. (+32+) : The ﬁrst case of Legionnaires’ disease in Japan. Kansensyogaku
Zasshi, //, +,.ῌ+,2.