NIPPON Genetics EUROPE GmbHwww.nippongenetics.eu
www.n-genetics.com

Source: Acinetobacter calcoaceticus

Restriction Enzyme
Acc I
Cat.#FG-AccI

Size
1,000 units

Reaction conditions
1X FastGene® Buffer IV, 37℃
1X FastGene® FastCut Buffer, 37℃
Conc.
4 units/μl

Store at -20℃
Supplied with: 10X FastGene® Buffer IV(FG-REB4)
10X FastGene® FastCut Buffer (FG-REBHF)
6X DNA Loading Buffer
Sterile water
Recognition site

5’ … G T M K A C … 3’
3’ … C A K M T G … 5’FastGene® FastCut Buffer
FastGene® restriction enzyme can cut substrate DNA in 5-15 minwith FastGene® FastCut Buffer.

1X FastGene® Buffer IV
20 mM Tris-acetate (pH 7.9 at 25℃)
50 mM potassium acetate
10 mM magnesium acetate
100 μg/ml BSA

Unit definition
One unit is defined as the amount of enzyme required forcomplete digestion of 1 μg bacteriophage λ at 37℃ for 1 hr in
50 μl reaction mixtures.

Quality control
For Research Use Only. Not for use in diagnostic procedures.

Dilution buffer
FastGene® Diluent
ANIPPON Genetics
EUROPE GmbHHeatInactivationwww.nippongenetics.eu- Unit definition assay- Overdigestion assay- Endonuclease assay- Extreme pure assay

Standard reaction condition- Normal protocol
Component

Final Conc.

VolumeAcc
I can be inactivated at 80℃ for 20 min.
www.n-genetics.com

Substrate DNA

1 μg

Methylation sensitivity

10X FastGene® Buffer IV

1X

5 μl

Acc I

4 unit1 μl

dam methylation: Not sensitive
dcm methylation: Not sensitive

Restriction Enzyme
Acc I incubation
ProlongedSource: Acinetobacter calcoaceticus

CpG methylation: Conditionally sensitive

Reactionconditions
Sterile water

A minimum amount of enzyme required to digest 1 μg substrate
Cat.#SizeConc.
DNA for 16 hr; 0.13 U.
FG-AccI
1,000 units
4 units/μl

FastGene®Component
FastCut Buffer

Relative activity in FastGene® Buffers

Store ®
atBuffer-20℃ I:FastGene
75%
100%
FastGene® Buffer II:®
FastGene Buffer III:100%
® Buffer IV
Suppliedwith:
10X
FastGene(FG-REB4)®
100%
FastGene Buffer IV:® FastCut Buffer (FG-REBHF)
FastGene
FastGene® FastCut 10X
Buffer:100%
6X DNA Loading Buffer
Sterile waterNote
It does not cleave DNA with 3 or fewer bases on each side ofthe Recognition
recognition site.
site At least 13 bases are required beyond theends of the recognition site for efficient cleavage. Cleavage ofmammalian genomic DNA is blocked by CpG methylation
overlapping its5’recognition
Both… G T Msequence.
K A C …
3’M13 and pUC19
contain a single
3’Acc… ICsite.
A K M T G … 5’up to 50 μl®
1X FastGene
IV, for37℃→ IncubateBufferat 37℃
1 hr
1X FastGene® FastCut Buffer, 37℃- Fast protocol

Final Conc.
VolumeFastGene® restriction enzyme can cut substrate DNA in 5-15 min
Substrate DNA
1 μg
X μl
®
with FastGene FastCut Buffer.
10X FastGene® FastCut Buffer
1X5 μl® Buffer IV
1XAcc
FastGene
I4 unit
1 μl
20 mM Tris-acetate (pH 7.9 @ 25℃)
Sterilewaterup
to50μl50 mM potassium acetate→ Incubate
at 37℃for 15 min
10 mM
magnesiumacetate
100 μg/ml BSA※ We recommend 5-10 units of enzyme per µg DNA and 1020 units for genomic DNA in a 1 h digest.

Unit definition

One unit is defined as the amount of enzyme required forcomplete digestion of 1 μg bacteriophage λ at 37℃ for 1 hr in
50 μl reaction mixtures.

Quality control
For Research Use Only. Not for use in diagnostic procedures.

X μl- Unit definition assay- Overdigestion assay- Endonuclease assay- Extreme pure assay