2013 PRODUCT CATALOGNEUROPHYSIOLOGICAL PRODUCTS
FOR laboratory RESEARCHPINNACLE TECHNOLOGY, INC. | 2721 OREGON STREET | LAWRENCE, KS 66046
WWW.PINNACLET.COM | PHONE (785) 832-8866 | FAX (785) 749-9214 | SALES@PINNACLET.COM

Thank you for choosing Pinnacle Technology for your research needs. We offer a range ofturn-key systems for neurophysiological studies using freely moving animals and are committed
to developing new tools that simplify measurement, reduce cost, and enable new research. In
addition, Pinnacle offers a host of supporting products ranging from cages to software analysis
suites. We pride ourselves in providing exceptional customer service and are available to assist
you with every stage of your research process.
By forging collaborative relationships with our clients, we are able to develop cutting-edge toolsthat improve and simplify your research. We look forward to working with you.
All the Best,

Donna A. Johnson
President and CEO

INFORMATION and policies
Website:www.pinnaclet.com

Phone:(785) 832-8866

Email:

sales@pinnaclet.com

Fax:(785) 749-9214

Shipping Address:

2721 Oregon Street
Lawrence, KS 66046
USA

Ordering Information:
General: Products may be ordered directly from Pinnacle Technology, Inc., or from one of our approved distributors (see: http://www.pinnaclet.
com/distributors.html). Some products may not be available in all countries.
Biosensors and Carbon Fiber Sensors: 3LQQDFOH7HFKQRORJ\,QFUHTXLUHV¿YHEXVLQHVVGD\VQRWLFHSULRUWRUHTXHVWHGGDWHRIVKLSPHQW
IRUELRVHQVRURUFDUERQ¿EHUVHQVRURUGHUVPayment Terms: Net 30 days from date of invoice for customers with established credit. Prepayment or COD may be required if credit has not been
established. Major credit cards are accepted. Unpaid balances are subject to a late-payment fee of 1.5% per month. Pro-forma invoices are available
for international orders.
Use of Products: All Pinnacle Technology, Inc., products are sold for laboratory research use only. Pinnacle Technology, Inc., products have not been
approved by any government agency for use in human subjects or human testing.
Shipping Information:
United States: All orders ship F.O.B. Lawrence, KS. Standard orders are shipped FedEx® Ground (biosensor orders are shipped FedEx® 2nd'D\ )UHLJKWFKDUJHVDUHDGGHGWRWKH¿QDOLQYRLFH
International: Purchaser is responsible for payment of all import duties, tariffs, taxes, insurance, and other related charges. Pinnacle
Technology, Inc., ships via the purchaser’s courier of choice (UPS®, FedEx®, DHL®) using the purchaser’s courier account number. Orders
WILL NOT BE SHIPPED without this information. Pinnacle Technology, Inc., accommodates orders shipped through domestic shipping brokers.
Product/Price Notices:3ULFHVDQGVSHFL¿FDWLRQVDUHVXEMHFWWRFKDQJHZLWKRXWQRWLFH
Product Return Policy: All product returns require a Return Merchandise Authorization (RMA) number. Contact a Pinnacle Technology, Inc.,
representative to obtain an RMA number and proper RMA documentation. Returns should be shipped to Pinnacle Technology, Inc., within 30 days of
RMA number issuance. RMA documentation must be included in the return shipment, and the customer is responsible for all shipping and handlingcharges. Standard items that have not been used or damaged may be returned within 10 days of original delivery for a credit or refund. A 15%
restocking charge will be deducted from the refund or credit at Pinnacle Technology, Inc.’s discretion. Pinnacle Technology, Inc., does not offer refunds
RUFUHGLWVRQVSHFLDOFXVWRPRUPDGHWRRUGHUSURGXFWVZLWKFXVWRPPRGL¿FDWLRQV$OOSURGXFWVUHWXUQHGIRUUHSDLURUUHSODFHPHQWPXVWEHVDQLWDU\cleaned appropriately, and securely packaged.
Warranty Information: In general, products are warranted against defects in material and workmanship. Purchasers must comply with Pinnacle
Technology, Inc.’s policy regarding returns. Refer to Pinnacle Technology, Inc.’s website (www.pinnaclet.com) for detailed warranty information.

TABLE OF CONTENTSSENSORS

4-8

Biosensors and Carbon Fiber Sensors

4

Guide Cannulas

5

Wireless System for Rats

6

Tethered System for Mice

7

Desktop Potentiostat

8

Calibration Kits

8

EEG/EMG
System Overview

9-14
9-11

Three-Channel Systems for Mice and Rats

12

Four-Channel Systems for Mice and Rats

13

EEG/EMG/Biosensor Systems for Mice and Rats

14

SLEEP DEPRIVATION

15SYNCHRONIZED VIDEO

16

SOFTWARE
Sirenia® Basic
Sirenia® Sleep Pro
Sirenia® Seizure Pro

FSCVSystem Overview
Tethered System for Mice
Wireless System for Rats

ADDITIONAL PRODUCTS
Cages, Stands, and Accessory Products
Handheld Isolated Potentiostat
Analog Adapters
Swivels/Commutators
Master Clock

17-19
17
18
19

20-21
20
21
21

22-23
22
23
23
23
23

SENSORS

Pinnacle biosensors
BIOSENSORS monitor real-time changes in neurochemical concentrations. With Pinnacle’s turn-key electronic and software systems,
users can record and analyze second-by-second concentration changes of CNS neurochemicals in freely moving animals.
Pinnaclecurrently

offersPlatinum-Iridium
Surfaceglutamate, glucose, lactate,choline,andIntegrated Ag/AgCl
Reference Electrode

350 μmfunction by the enzymemediated processing of theanalyte of interest.

This

Pt-Ir Sensing Cavity

176 μmhydrogen peroxide that isthen detected by oxidation
Pt-Irelectrode.

Electroactive

interferents

1 mm

present in the brain are
excludedvia

Outer-Diffusion
Limiting Membrane *

a

BSA

e-

H2O2

e-

H2O2

e-

H2O2

e-

H2O2

PTFE Coated Electrode

240 μmresults in the production ofaEnzymeLayerethanolbiosensors. Our biosensors

atInner-Selective
Membrane

Epoxy Tip

Analyte ofInterest~200 - 225 μm
Interferent

passive* Outer-diffusion limiting membrane may not be present on all Pinnacle biosensors

selective membrane andthrough active removal when necessary.

COMMON USES

2XUVHQVRUV DUH VKLSSHG ZLWKLQ ¿YH EXVLQHVV GD\V RIRUGHU UHFHLSW
and include a warranty. Custom sensor sizes are also available.

In vivo monitoring of brain chemical microenvironments

Contact Pinnacle for details.

BIOSENSOR CHARACTERISTICS
In Vivo Lifetime

Limit of Detection

Glutamate

36 hours

0.05 - 0.1 μM

Glucose96+ hours

2 - 5 μM

Lactate96+ hours

5 - 10 μM

Ethanol

6 - 8 hours

0.1 - 0.5 μM

Choline8+ hours

0.05 - 0.1 μM

Neurochemical monitoring during behavioral and physiologicalactivities
Drug screening, including neuropharmacological effects,GHQWL¿FDWLRQRIELRPDUNHUV
Investigating cognition, behavior, circadian cycles, stress, learning,memory, sleep, seizure, vigilance state, and new drug effects

Real-time
Data
Low
AnalyteConsumption

High
Specificity
CARBON FIBER SENSORS (CFS) are used in conjunction

Advantages

with Pinnacle’s tethered and wireless potentiostats to measure
WKH SUHVHQFH RI WRWDO ELRJHQLF DPLQHV LQ WKH EUDLQ XVLQJ ¿[HG
potential amperometry (FPA). They are also used with fast scan
cyclic voltammetry (FSCV) systems. All Pinnacle CFSs requirean Ag/AgCl reference electrode (7065). The sensors are 34 μm
in diameter and 0.5 mm in length.

4

No sample
Post-processing

Fast
Sample Rate
Fast
Response TimeSENSORS

Biosensors and guide cannulas
BIOSENSORSDUHSXUFKDVHGE\FDQQXODW\SHDQGDQDO\WHRILQWHUHVWFDUERQ¿EHUVHQVRUVDUHSXUFKDVHGE\FDQQXODW\SH3LQQDFOH
recommends the use of a guide cannula system for optimal results when implanting and recording from sensors in the brain of a freely
moving animal. We manufacture standard biosensor electrodes (Pt-Ir wire with an integrated Ag/AgCl reference) that are compatible
with two guide cannula types: BASi cannulas for rats and smaller BASi

Guide cannula types

cannulas for mice. Sensors with no cannula are also available for

Product

Item #

BASi guide cannula for rats

7030

BASi guide cannula for mice

7032

purchase. All sensors sold by Pinnacle are for non-human use only.

Sensor types
Product

Item #
7001

No cannula headpieceBASi RAT CANNULA
End of Guide Cannula with Inserted Biosensor

O Ring19.5 mm

Guide Cannula Shaft: 0.7 mm

7001-Choline

7001-Glutamate

7001-CFS *7001-Ethanol

7001-Lactate

7001-CFS-F **7001-Glucose

BASi cannula headpiece for rats (wireless)

7002

BASi cannula headpiece for mice

7004

BASi cannula headpiece for rats (tethered)7011

4.6 mm

2.5 mm from end ofguide shaft to midpointof sensing cavity

10 mm

*

Sensing Cavity: 1 mm

7001-CFS: Carbon Fiber Sensor
&)6)&DUERQ)LEHU6HQVRU ¿[HGLQFDQQXOD$OOVHQVRUW\SHVFDQEHRUGHUHGLQDQ\RIWKHDQDO\WHVFRQ¿JXUDWLRQV

BASi MOUSE CANNULA

listed under 7001. For example, a glutamate biosensor with a BASicannula headpiece for mice is ordered as 7004-Glutamate.

End of Guide Cannula with Inserted Biosensor

2.5 mmGuide Cannula Shaft: 0.65 mm

6 mm~1.3 mm from end ofguide shaft to midpointof sensing cavity

3.2 mm

5 mm

Custom sensors are available
Contact a Pinnacle representativeat (785) 832-8866

Sensing Cavity: 1 mm

glutamate interference response18ȝ0
L-Glutathione

12

ȝ0
5-HT

ȝ0
GABA

ȝ0
L-Aspartic
Acid

16

ȝ0
L-Cysteine

10
8
10 ȝ0
Glutamate

6

ȝ0
NE

ȝ0
Tryptophan

ȝ0
DA

ȝ0
L-Tyrosineȝ0L-Glutamineȝ0Ascorbate

selective

fast

4
ȝ0
Glutamate2linear14

Current (nA)14

Oxidation Current (nA)Glutamate Biosensor Linearity

20

12
10
8
6
4
2
0

0
0

100

200

300

400

500

600

700

Time (s)

0

5

10

15

20

25

30

35

40

45

50

Glutamate (μM)

Pinnacle biosensors are selective for the analyte of interest and are linear at normal in vivo oxygenation levels over a physiologically5

relevant range, as shown in the diagrams above.

5

SENSORS

Wireless system for rats
The BLUETOOTH® POTENTIOSTAT SYSTEM uses Bluetooth® technology to wirelessly record up to two biosensors simultaneously in
one animal. The battery-powered potentiostat, housed in Pinnacle’s headmounted Rat Hat, communicates with a paired USB dongle up
to seven meters away. Multiple potentiostats can be used in one room, allowing for high capacity biosensor studies.

1
4

Stereotaxically placed guide cannula(s) allow for biosensor insertion postsurgery.

2

The wireless system uses a Rat Hat to house the electronics, battery, guide
FDQQXOD DQG ELRVHQVRU 7KH 5DW +DW ERWWRP LV DI¿[HG WR WKH VNXOO ZLWK ERQH
screws and dental acrylic.

33

A low-powered, wireless, two-channel potentiostat applies a bias and transmits
up to two digitized signals to a paired Bluetooth® USB dongle that interfaces with

BLE 1

M

Pinnacle’s Sirenia® Acquisition software for data recording.
4
2

The Rat Hat top protects the system, allowing for group housed and behavioral
experiments.

1

Key features
Weight: 11.8 grams

Transmission Radius: 6 meters

Supports up to 2 simultaneous biosensor recordings

Record from Multiple animals within same Room

Accessory kit
8100-K7:

Bluetooth® Potentiostat Accessories

7030 - BASi cannula for rats (4)8134-20 - Test load (2)7035-R-BAS - Probe holder for BASi rat cannula8154-72 - 8172 template

8107-BLE - Rat Hat top

8241-S - Screwdriver for 1/8” screws

8108-BLE - Rat Hat bottom (4)9005 - 7-port powered USB hub

8172 - Bluetooth potentiostat

8111 - 1/8” screws (pkg. of 12)9031 - Battery (pkg. of 5)9052 - USB extension cable8112 - Drill bit (2)9054 - Bluetooth® dongle

Contact Pinnacle for dual biosensor implantation accessories. Biosensors sold separately.

Hardware kit
8100-K5:®

Bluetooth Potentiostat System®

Software and manuals are also included.

Tethered systems also available

disposable

items

6

For rats
Item #

Product

7030

BASi rat guide cannula8108-BLE

Rat Hat bottom

8111

1/8” screws (pkg. of 12)

8112

Drill bit9031

Battery for potentiostat (pkg. of 5)

SENSORS

Tethered system for mice
The MOUSE BIOSENSOR SYSTEM IHDWXUHVFRQ¿JXUDEOHLQSXWFKDQQHOVWRUHFRUGQHXURFKHPLFDOFRQFHQWUDWLRQV7KLVWHWKHUHGV\VWHPHPSOR\VDKHDGPRXQWHGSUHDPSOL¿HUIRUPHDVXULQJXSWRWZRELRVHQVRUVVLPXOWDQHRXVO\LQRQHDQLPDOSURYLGLQJDWXUQNH\VROXWLRQIRU
biosensor recordings in mice.

1
4

Stereotaxically placed guide cannulas allow for the insertion of biosensors postVXUJHU\3UHIDEULFDWHGKHDGPRXQWVDUHDI¿[HGWRWKHVNXOOZLWKGHQWDODFU\OLFDQG
DFWDVDFRQQHFWLRQSRUWIRUWKHWZRFKDQQHOELRVHQVRUSUHDPSOL¿HU

3

2+HDGPRXQWHGSUHDPSOL¿HUVKRXVHWZRFRQQHFWRUVIRUELRVHQVRUVDQGDUHNH\WR
quality, artifact-free data.

I

T

3

A low-torque commutator allows for unencumbered freedom of movement.

4

7KHGDWDFRQGLWLRQLQJDQGDFTXLVLWLRQV\VWHPSHUIRUPVVHFRQGDU\DPSOL¿FDWLRQ

®

Products

DQG¿OWHULQJEHIRUHVHQGLQJGDWDWR3LQQDFOH¶V6LUHQLD® Acquisition software forZ19collection.

2

Key features
Low torque

Weight: 2.2 grams

Headmounted amplification

Supports up to 2 simultaneous biosensor recordings

1

Accessory kits
8400-K3-BIO:

Hardware kit
8400-K1-2BIO:

Tethered Mouse Biosensor System

8401 - Data conditioning and acquisition system
8406-2BIO - 0RXVHSUHDPSOL¿HUIRUXSWRWZRELRVHQVRUVTethered Accessory Kit for One Biosensor

7032 - BASi mouse cannula (6)8209 - 0.10” screws (3 pkgs. of 8)7033 - Bio-only headmount (6)8241-F - Screwdriver for mouse screws

7035-M-BAS - Probe holder for mouse cannula

8254 - 23-gauge needle (6)8134 - Test load (2)9005 - 7-port powered USB hub

8400-K3-2BIO:

Tethered Accessory Kit for Two Biosensors

8408 - Mouse commutator/swivel with 18” mounting plate
Components of this kit are the same as above except for the quantities of 7032 (12) and 7035-M-BAS (2).
Cables for one animal, software, and manuals are also included.

Biosensors sold separately.

For mice

7

Item #

Product

7032

BASi mouse guide cannula

7033

Bio-only headmount8209

0.10” screws (pkg. of 8)8254

23-gauge needle

Add synchronized video
See page 16 for details.

7

SENSORS

Desktop potentiostat
PINNACLE’S FOUR-CHANNEL DESKTOP POTENTIOSTAT provides a cost-effective, easy-to-use, and highly accurate system forthe development and use of high impedance, amperometric biosensors and biosensor arrays. It is well suited for anesthetized animal
experiments, brain slices, and other in vitro studies. The system is compatible with Pinnacle’s biosensors and third-party sensors.

Hardware kit
8100-K4:

Desktop Potentiostat System

8102 - Desktop potentiostat
8109 - Sensor adapter cables (pkg. of 4)

Key features

8118 - Power supply

Four independent channels

Maximum sampling rate: 4 Hz

8124 - Shorting cap

7ZRWHUPLQDO¿[HGSRWHQWLDOCurrent range: 0 - 20 uA

8125 - BNC to alligator leads cable (4)Synchronous channel sampling

Bias range: -2.048 to +2.048 V

8155 - BNC test load (4)9005 - 7-port powered USB hub
Cables, software, and manuals are also included.

Compatible with third-party sensors

Calibration kits
In order to relate the in vivo current changes measured by a biosensor to actual changes
in analyte concentration, it is necessary to calibrate the biosensor at the conclusion of thein vivo experiment. Pinnacle offers a number of in vitro calibration systems that allow thesimultaneous calibration of up to four biosensors.

Calibration kits
Product

Kit #Tethered mouse in vitro calibration kit

7000-K1-T

Wireless rat in vitro calibration kit (use with BASi cannulas)

7000-K2-W-BAS

Correlating current to concentration
Shown in the image on the left are in vivo recordings from
two alcohol biosensors implanted contralaterally in the cortex
of a Wistar rat. An ethanol bolus (1 g/kg) was delivered at
the sixty-minute mark. Data were transformed to changes
in ethanol concentration based on each sensor’s postcalibration. INSET: The raw, untransformed current (nA) fromthe two alcohol biosensors as acquired through Pinnacle’s
acquisition software.

8EEG/EMG SYSTEMS

EEG/EMG SYSTEMSTHREE-CHANNEL AND FOUR-CHANNEL BIOPOTENTIAL RECORDING SYSTEMS are available for sleep, seizure, and general
EHKDYLRUDOSDUDGLJPVLQIUHHO\PRYLQJPLFHDQGUDWV%RWK((*(0*V\VWHPVXVHKHDGPRXQWHGSUHDPSOL¿HUVWRSURGXFHH[FHSWLRQDOO\clean waveforms, even during animal movement. The four-channel system provides all the great features of the three-channel system
DORQJZLWKDQH[WUDFKDQQHOFRQ¿JXUDWLRQÀH[LELOLW\DQGWKHDGGHGFDSDELOLW\RILQFRUSRUDWLQJVLPXOWDQHRXVELRVHQVRUPHDVXUHPHQWV
6HHWKH³6\VWHP)HDWXUHV´FKDUWWRGHWHUPLQHZKLFKV\VWHPEHWWHU¿WV\RXUUHVHDUFKQHHGV

two turn-key systems
SYSTEM FEATURES

3 CHANNEL

4 CHANNEL

Available for both mice and rats
Optimized for sleep and seizure experiments
No cable artifact$GMXVWDEOHJDLQDQGORZSDVV¿OWHUVSampling rate up to 2000 Hz per channel
Digital input/output controls
Analog output option)XOO\FRQ¿JXUDEOHFKDQQHOVBiosensor support
5HFRQ¿JXUHYLDSUHDPSOL¿HUH[FKDQJH

ƒ6\VWHP%UHDNGRZQ +RZ3UHDPSOL¿HUV:RUN Pages 10-11
ƒThree- & Four-Channel Systems, Hardware & Accessory Kits: Pages 12-13
ƒ$GGD%LRVHQVRUWR((*(0*5HFRUGLQJV Page 14

COMMON
USES

zzz

F
S

SLEEP STUDIESSEIZURE RESEARCH

DEPTH ELECTRODESCORTICAL RECORDINGSCOGNITIVE STUDIESLOCAL FIELD POTENTIAL

low noise
SYNCHRONIZEDVIDEO

TURN-KEY
SYSTEMADVANTAGES
advancedanalysis tools

simplesurgeries
FREE ACQUISITIONSOFTWARE

9

Two channels of EEG data can be captured alongside EMG data using Pinnacle’s three-channel
biopotential recording system.

9EEG/EMG SYSTEMSSYSTEM BREAKDOWNOUR TURN-KEY SYSTEMS DUHHQJLQHHUHGWRGHOLYHUFOHDQDUWLIDFWIUHHGDWD((*DQG(0*ZDYHIRUPVDUHDPSOL¿HGDQG¿OWHUHGDW
WKHKHDGRIWKHDQLPDOE\DSUHDPSOL¿HU6LJQDOVDUHWKHQSDVVHGWKURXJKWKHORZWRUTXHVZLYHOWRWKHGDWDFRQGLWLRQLQJDQGDFTXLVLWLRQV\VWHPIRU¿QDOVWDJHDPSOL¿FDWLRQDQG¿OWHULQJ(DFKFKDQQHOLQRXUWKUHHDQGIRXUFKDQQHOV\VWHPVIHDWXUHVLQGHSHQGHQWDGMXVWDEOH
JDLQDQG¿OWHUVHWWLQJV
Data are collected using Pinnacle’s free acquisition software, Sirenia®. The software allows users to view EEG/EMG recordings in userGH¿QHGWLPHSHULRGVPDQXDOO\VFRUHVOHHSDQGUHYLHZVHL]XUHHYHQWV$OOGDWDFDQEHFRQ¿JXUHGIRUH[SRUWWRPRVWVSUHDGVKHHWDQG
database programs and are compatible with our advanced analysis software packages. See pages 17-19 for additional information on
Pinnacle software.
DATA CONDITIONING AND ACQUISITION

tethered systems for rats

A data conditioning and acquisition system (DCAS) performs secondary
DPSOL¿FDWLRQDQG¿OWHULQJEHIRUHVHQGLQJGDWDWR3LQQDFOH¶V6LUHQLD® Acquisition
software for collection via a USB connection.

SPECIFICATIONSCOMMUTATORA Plastics One commutator is mounted above theƒAdjustable Sampling Rates: 200 - 2,000 Hzƒ6RIWZDUH&RQ¿JXUDEOH/RZ3DVV)LOWHUV 10 Hz - 1 kHz

cage. The commutator’s two-plug setup allows forŶ

even rotation of the rotor.
ŶPRODUCT PART NUMBER KEY:
3-Channel SystemŶ 4-Channel System

CABLE

1

An 18” tether from Plastics One connects the
FRPPXWDWRUWRWKHSUHDPSOL¿HU7KHFDEOH¶VZLUHV
are protected by a metal spring coil.

2
PREAMPLIFIER
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delivery of clean, artifact-free data. A Plastics One
VFUHZFRQQHFWRULVXVHGWRVHFXUHWKHSUHDPSOL¿HU

I

to the head of the animal.
Ŷ3

Z19

RAT HEADMOUNT

4
Prefabricated rat headmounts use Plastics
2QH ¿WWLQJV PRXQWHG RQ D  PP ;  PP
board with EEG or EMG electrode wires
attached. An additional two-pin electrode is
XVHGIRU((*FRQ¿JXUDWLRQV

10

5

T
®

Products

EEG/EMG SYSTEMSHEADMOUNTED AMPLIFICATION=

CLEANER DATAHow Our preamplifiers Work
Gain and High-Pass filters
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measurements between two electrodes. Each channel also
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tethered systems for micegain

high-pass filters

SeizureX100

1.0 Hz

Sleep

X100

0.5 HzSeizure

X100

10 Hz

Sleep

X100

10 Hz

mouse CONFIGURATIONs
EEG Channel(s)EMG Channel

COMMUTATOR

rat CONFIGURATIONs

A low-torque commutator, which is mounted above
the cage, allows for unencumbered freedom of

gain

high-pass filters

SeizureX10

1.0 HzEEG Channel(s)movement.
Rotational Torque: <2 x 10-4 N-m
Ŷ

Sleep

X100

0.5 HzSeizure

X10

10 Hz

Sleep

X100

10 Hz

EMG Channel

Shared and fully independent Channels

CABLE

6WDQGDUG WKUHH DQG IRXUFKDQQHO SUHDPSOL¿HUV KDYH WZRchannels sharing a common electrode and either one or

A 14” tether connects the commutator to the
SUHDPSOL¿HU 6L[ LQVXODWHG ZLUHV DUH EDQGHG

two independent channels, respectively. Fully independent,

together to create this lightweight cable that is

GLIIHUHQWLDO SUHDPSOL¿HUV DUH DOVR DYDLODEOH 6HH GLDJUDPV

ideal for use with very small animals.

below.
Perspective: 3LQVH[WHQGLQJIURPSUHDPSOL¿HU

PREAMPLIFIER
6LJQDOV DUH DPSOL¿HG DQG ¿OWHUHG DW WKH KHDG RI
WKH DQLPDO XVLQJ RXU SUHDPSOL¿HUV 7KLV HQVXUHVthe delivery of clean, artifact-free data. The mouse
SUHDPSOL¿HUFRQQHFWVWRDKHDGPRXQWYLDDIULFWLRQ¿WŶ
Three-Channel System
6WDQGDUG&RQ¿JXUDWLRQ
(For Mice)Three-Channel System)XOO\,QGHSHQGHQW&RQ¿JXUDWLRQ
(For Mice)

Four-Channel System
6WDQGDUG&RQ¿JXUDWLRQ
(For Mice)

MOUSE HEADMOUNTCHANNEL 1
CHANNEL 2

Prefabricatedheadmounts

or

FRQQHFWRUVVXSSRUWÀH[LEOH

for reproducible electrodeelectrode placement forplacement,

customizable cortical or

andprovidedepth recordings.

SHAREDGROUND

eight-pin

reduce surgery time, allow

ready-to-insert EMG leads.

11

Six-pin

CHANNEL 3
CHANNEL 4

CUSTOM CONFIGURATIONS AVAILABLE
Contact a Pinnacle representativeat (785) 832-8866

11

EEG/EMG SYSTEMSTHREE CHANNEL SYSTEMS
Pinnacle’s THREE-CHANNEL TETHERED SYSTEM allows researchers to simultaneously record three channels of EEG and/or EMG
GDWD7KHGDWDFRQGLWLRQLQJDQGDFTXLVLWLRQV\VWHP '&$6 DQGSUHDPSOL¿HUDUHSUHFRQ¿JXUHGDQGRUGHUHGDVDPDWFKLQJSDLU6WDQGDUGFRQ¿JXUDWLRQVLQFOXGH((*(0*FKDQQHOVIRUVOHHSRUVHL]XUHVWXGLHVDQG((*FKDQQHOVIRUVHL]XUHUHVHDUFK)XOO\LQGHSHQGHQWSUHDPSOL¿HUVDUHDOVRDYDLODEOH/HDUQPRUHDERXWSUHDPSOL¿HUVDQGKRZRXUWXUQNH\V\VWHPVZRUNRQSDJHVACCESSORY KITS

systems for mice

8200-K3-SL/SE:

HARDWARE KITS
&RQ¿JXUDWLRQ
2 EEG/1 EMG

2 EEG/1 EMG for Sleep or Seizure

8201 - Mouse headmount (4)8249 - Test source

Sleep

Seizure8209 - 0.10” screws (pkg. of 8)8254 - 23-gauge needle (4)8200-K1-SL

8200-K1-SE

8212 - 0.12” screws (pkg. of 8)8255 - Multimeter

8200-K1-iSE

8226 - Twin pack of silver epoxy (2)3 EEG

8200-K1-SE3

8241-F - Screwdriver for EEG screws

3 EEG*

8200-K1-iSE3

2 EEG/1 EMG*

A

8200-K1-iSL

Contents:
8202 - 0RXVHSUHDPSOL¿HU
8204 - Mouse commutator/swivel with 14” mounting plate
8206 - Data conditioning and acquisition system

8200-K3-iSL/iSE:

9005 - 7-port powered USB hub

2 EEG/1 EMG for Sleep or Seizure*

Components of this kit are the same as above except 8201 is replaced with
8431. In addition, it contains an 8-pin to 6-pin adapter (8272) and 20- 0.10”
screws with wire leads (8403) instead of products 8209, 8212, and 8226.

8200-K3-SE3:
Cables for one animal, software, and manuals are also included.
NOTE: Products 8202 and 8206 come in multiple varieties:
SL, SE, SE3, DSL*, DSE*, and DSE3*.

Posterior

2 EEG / 1 EMG for Mice

3 EEG for Seizure
Posterior

3 EEG for Mice

Components of this kit are the same as above except 8201 is replacedwith 8235-SM. In addition, it contains 24- 0.10” screws with wire leads(8403) instead of products 8209, 8212, and 8226.

* Fully Independent

8200-K3-iSE3:

ACCESSORY KITS

HARDWARE KITS
2 EEG/1 EMG
3 EEG

Sleep
8200-K2-SL

orComponents of this kit are the same as above except 8201 is replaced with
8415-SM. In addition, it contains an 8-pin to 6-pin adapter (8272) and 280.10” screws with wire leads (8403) instead of products 8209, 8212, and 8226.

systems for rats
&RQ¿JXUDWLRQ

3 EEG for Seizure*

Seizure8200-K2-SE
8200-K2-SE3

Contents:
8206 - Data conditioning and acquisition system
8213 - 5DWSUHDPSOL¿HU
8214 - Rat commutator/swivel with 14” mounting plate

8200-K4-SL/SE:P

2 EEG / 1 EMG for Rats

2 EEG/1 EMG for Sleep or Seizure

8112 - Drill bit

8249 - Test source

8239 - Rat headmount (4)8255 - Multimeter

8241-S - Screwdriver for 1/8” screws

9005 - 7-port powered USB hub

8247 - 1/8” screws with wire leads (16)8200-K4-SE3:

All accessory kits contain items needed
for completion of four or six surgeries.

3 EEG for Seizure

All quantities are (1) unless otherwise
Cables for one animal, software, and manuals are also included.
NOTE: Products 8206 and 8213 come in three varieties:
SL, SE, and SE3.

have your own amplifier?
Learn more about using Pinnacle’s
SUHDPSOL¿HUVZLWKWKLUGSDUW\V\VWHPV
on page 23.

12

noted after the product description.

Components of this kit are the same as above except for the quantity of 8247
(24). In addition, 8239 is replaced with 8239-SE3.

disposable

items

For rats
Item #

Product

8112

Drill bit8239

2 EEG/1 EMG rat headmount8239-SE3

3 EEG rat headmount8247

1/8” screws with wire leads

8425

2-pin electrodeEEG/EMG SYSTEMSFOUR CHANNEL SYSTEMSThe FOUR-CHANNEL TETHERED SYSTEM VXSSRUWVXSWRIRXUELRSRWHQWLDOLQSXWFKDQQHOV7KLVV\VWHPLVDPRQJWKHPRVWÀH[LEOHRI
3LQQDFOH¶VKDUGZDUHGHYLFHVEHFDXVHXVHUVFDQHDVLO\PRGLI\WKHFRQ¿JXUDWLRQE\FKDQJLQJRQO\WKHSUHDPSOL¿HU6WDQGDUGFRQ¿JXUDWLRQV
include 3 EEG/1 EMG channels and 4 EEG channels for seizure research. )RUV\VWHPDQGSUHDPSOL¿HUVSHFL¿FDWLRQVUHIHUWRSDJHV

systems for mice
Anterior&RQ¿JXUDWLRQ

Posterior

ACCESSORY KITS

HARDWARE KITS

3 EEG/1 EMG for Seizure

3 EEG/1 EMG

8400-K1-SE31M

8241-F - Screwdriver for EEG screws

8403 - 0.10” screws with wire leads (36)4 EEG

8400-K1-SE4

8249 - Test source

8431 - 3 EEG/1 EMG headmount (6)8254 - 23-gauge needle (6)8432 - 8-pin to 6-pin adapter

8255 - Multimeter

9005 - 7-port powered USB hub

Contents:

4 EEG for Mice

8400-K3-SE31M:

SeizureSleep

8401 - Data conditioning and acquisition system
8406 - 0RXVHSUHDPSOL¿HU
8408 - Mouse commutator/swivel with 18” mounting plate

Cables for one animal, software, and manuals are also
included. NOTE: Product 8406 comes in multiple varieties:
SE31M and SE4.

8400-K3-SE4:

4 EEG for Seizure

Components of this kit are the same as above except for the quantity of 8403
(48). In addition, product 8431 is replaced with 8415-SM.

Posterior

4 EEG for Rats

simple surgeriessystems for rats

Pinnacle’s prefabricated headmounts and&RQ¿JXUDWLRQIRU FRQQHFWLQJ HOHFWURGHV WR SUHDPSOL¿HUV6WDLQOHVV VWHHO VFUHZV DI¿[ WR WKH VNXOOACCESSORY KITS

HARDWARE KITS

connectors provide fast and easy solutions

Sleep

4 EEG

Seizure8400-K2-SE4

doubling as anchors and electrodes for EEG
Contents:

data acquisition. Depth electrodes can be

8401 - Data conditioning and acquisition system
8407 - 5DWSUHDPSOL¿HU
8409 - Rat commutator/swivel with 18” mounting plate

soldered to connectors for LFP recordings.
)RU

FRQ¿JXUDWLRQVVXSSRUWLQJPXVFOH

movement, EMG leads easily insert into the

8400-K4-SE4:

4 EEG for Seizure

8112 - Drill bit

8249 - Test source

8239-SE3 - 3 EEG headmount (4)8255 - Multimeter

8241-S - Screwdriver for EEG screws

8425 - 2-pin electrode (4)8247 - 1/8” screws with wire leads (24)9005 - 7-port powered USB hub

back or neck muscles.
Cables for one animal, software, and manuals are also included.
NOTE: Product 8407 comes in multiple varieties. This kit includes
an 8407-SE4.

+HDGPRXQWHG$PSOL¿FDWLRQ Ensures clean, artifact-free data
Ease of Use: Systems are easily assembled for immediate use
Turn-Key System: Provides complete solutions for data acquisition and analysis

For mice

13

Item #

Product

Item #

Product

8201

Mouse headmount8254

23-gauge needle

8209

0.10” screws (pkg. of 8)8403

0.10” screws with wire leads

82120.12” screws (pkg. of 8)8415-SM8-pin connector

8226

Twin pack of silver epoxy

8431

3 EEG/1 EMG headmount8235-SM6-pin connector

add synchronizedvideo
See page 16 for details.

13EEG/EMG SYSTEMSADD A BIOSENSOR CHANNELANY PINNACLE BIOSENSOR can be used in conjunction with our four-channel EEG/EMG system for recording up to two simultaneous
biosensor channels. Adding biosensors enables the correlation of biopotential and neurochemical activity in freely moving mice and rats,
PDNLQJWKHV\VWHPSHUIHFWIRUXQORFNLQJQHZ¿QGLQJVGXULQJVOHHSVHL]XUHDQGEHKDYLRUDOVWXGLHV,WLVDOVRSRVVLEOHIRUWKHV\VWHP
to acquire two biosensor inputs on a single channel. This allows for the simultaneous capture of data from three biopotential and twobiosensor signals. Learn more about biosensors on pages 4-5.

systems for micesystems for rats
HARDWARE KITS

HARDWARE KITS
&RQ¿JXUDWLRQ

Sleep

Seizure&RQ¿JXUDWLRQ

Sleep

Seizure1 EEG/1 EMG/2 Biosensor

8400-K1-SL-2BIO

8400-K1-SE-2BIO

2 EEG/1 EMG/1 Biosensor

8400-K2-SL

8400-K2-SE

2 EEG/1 EMG/1 Biosensor

8400-K1-SL

8400-K1-SE

3 EEG/1 Biosensor

2 EEG/1 EMG/2 Biosensor

8400-K1-5SL

8400-K1-5SE

Contents:

3 EEG/1 Biosensor

8400-K1-SE3

3 EEG/2 Biosensor

8400-K1-5SE3

8400-K2-SE3

8401 - Data conditioning and acquisition system
8407 -5DWSUHDPSOL¿HU
8409 - Rat commutator/swivel with 18” mounting plate

Contents:
Cables for one animal, software, and manuals are also included.
NOTE: Product 8407 comes in multiple varieties: SL, SE, and SE3.

8401 - Data conditioning and acquisition system
8406 -0RXVHSUHDPSOL¿HU
8408 - Mouse commutator/swivel with 18” mounting plate

Cables for one animal, software, and manuals are also included.
NOTE: Product 8406 comes in multiple varieties: SL, SE, SE3,
SL-2BIO, SE-2BIO, 5SL, 5SE, and 5SE3.

ACCESSORY KITS
8400-K3-SL/SE-2BIO:

1 EEG/1 EMG/2 Biosensor for Sleep or Seizure

7032 - BASi mouse cannula (12)8254 - 23-gauge needle (6)7035-M-BAS - BASi cannula probe holder (2)8255 - Multimeter

8134 - Test load (2)8402 - EEG/EMG/Bio headmount (6)8209 - 0.10” screws (2 pkgs. of 8)8403 - 0.10” screws with wire leads (12)8241-F - Screwdriver for EEG screws

8429-M - Mouse BASi stylet ground8249 - Test source

9005 - 7-port powered USB hub

8400-K3-SL/SE:

ACCESSORY KITS
8400-K4-SL/SE-BAS:

2 EEG/1 EMG/1 Biosensor for Sleep or Seizure

7030 - BASi rat cannula (4)8247 - 1.8” screws with wire leads (12)7035-R-BAS - BASi cannula probe holder

8249 - Test source

8111 - 1/8” screws (pkg. of 12)8255 - Multimeter

8112 - Drill bit

8427 - Test load (2)8239 - Rat headmount (4)8429-BAS - BASi stylet ground8241-S - Screwdriver for EEG screws

9005 - 7-port powered USB hub

8400-K4-SE3-BAS:

3 EEG/1 Biosensor for Seizure

Components of this kit are the same as above except for the quantity of 8247 (20). In
addition, product 8239 is replaced with 8239-SE3.

2 EEG/1 EMG/1 Biosensor for Sleep or Seizure

Components of this kit are the same as above except quantities for 7032 (6),7035-M-BAS (1), 8209 (1 pkg. of 8), and 8403 (18).

8400-K3-5SL/5SE:

2 EEG/1 EMG/2 Biosensor for Sleep or Seizure

Combined EEG/EMG/Biosensor Systems

Components of this kit are the same as above except quantities for 8209 (1 pkg. of 8)
and 8403 (18).

Lactate
100 μM

8400-K3-SE3:

3 EEG/1 Biosensor for Seizure
EEG

GlucoseComponents of this kit are the same as above except quantities for 7032 (6), 7035-MBAS (1), 8209 (1 pkg. of 8), and 8403 (30). In addition, product 8402 is replaced with
8235-SM.

250 μV

EMG

8400-K3-5SE3:

3 EEG/2 Biosensor for Seizure

Components of this kit are the same as above except quantities for 8209 (1 pkg. of 8)
and 8403 (30). In addition, product 8402 is replaced with 8235-SM.

Biosensors sold separately.

14

All accessory kits contain items needed for completion of four or six surgeries.
All quantities are (1) unless otherwise noted after the product description.

00.5

1.0 Hours

1.5

2.0

2.5

EEG and EMG waveforms are plotted simultaneously with calibrated biosensor
traces for lactate and glucose recorded from a single animal.

SLEEP DEPRIVATIONSLEEP DEPRIVATION SYSTEM
Pinnacle’s AUTOMATED SLEEP DEPRIVATION SYSTEM offers a unique solution for sleep deprivation and fragmentation studiesof mice and rats by eliminating the need for direct human intervention. The rotation of a metal bar positioned above the cage bedding
disturbs the animal in a manner similar to gentle handling, preventing sleep without imposing forced exercise. Studies have shown thesystem to be an effective method for both long-term sleep fragmentation and total sleep deprivation studies. It is sold as either a Stand
Alone System or a Feedback System, which uses EEG/EMG activity to determine sleep state.

Stand alone system
Pinnacle’s Stand Alone Sleep Deprivation system allows users to program thesystem to turn on or off according to the research schedule. Researchers canuse Pinnacle’s EEG/EMG and biosensor recording systems or use the system
in conjunction with third-party recording systems. The software scheduler canbe set to a second-by-second basis, allowing for sleep fragmentation studies.
For shift work simulation studies, each system can be programmed to turn
RQRURIIRQDQKRXUO\GDLO\ZHHNO\RUPRQWKO\XVHUGH¿QHGVFKHGXOH2QFH
the schedule is set, the unit operates independently and can be moved to any
location. Users can upgrade to feedback mode at any time.

feedback system
The Feedback System provides all the functionality of the Stand Alone systemalong with the capability of adding real-time EEG and EMG feedback to ensure
the bar rotates only when the animal is entering a sleep-like state. Rotation of

automated sleep deprivation systems

WKHEDUFHDVHVRQFHWKHDQLPDOKDVEHHQDZDNHIRUDXVHUGH¿QHGSHULRGRI

Product

Item #

time, limiting excessive exercise and stress effects normally associated withStand Alone for Mice

9000-K5-S

constant stimulus applications.

Feedback for Mice

9000-K5

Stand Alone for Rats

9000-K6-S

Feedback for Rats

9000-K6

In Feedback Mode, users can perform yoked controlled experiments in which
multiple sleep deprivation systems can be slaved together to operate under auniform set of experimental parameters. When connected together in software,
two or more systems can be programmed to turn on or off simultaneously.

100

Key features
Performs short-term, long-term, or
chronic partial sleep deprivation studies
Automatic feedback and stand-alone
modes
Yoked control functionality

Minutes of Sleep/2h

Gently restricts sleep without
unnecessary exercise or stress

Baseline90

Sleep Deprivation

80
70
60
50
40
30
20
10

Minimizes resources needed to manually
sleep deprive animals

0
0-1

2-3

4-5

6-7

8-9

10-11

12-13

14-15

16-17

18-19

20-21

22-23

Hours of Recording

EFFECTIVENESS OF SLEEP DEPRIVATION SYSTEM ON MICE
Comparison of baseline sleep (purple bars) with a 24-hour sleep deprivation period (gold bars) using rotating bar and active EEG feedback. Six mice (male, C57BL6) were maintained on a 12-hour lights-on(horizontal tan)/ 12-hour lights-off (horizontal black) schedule. EEG and EMG activity was recorded for 24 hours (baseline). During the following 24 hours (SD), baseline data were used to program individual
sleep deprivation units to rotate when the mouse entered a sleep-like state. Vertical bars represent sleep time in 2-hour bins.

15

15

SYNCHRONIZED VIDEO

synchronized video capability
synchronized video systems

Pinnacle’s VIDEO SYSTEM can record up to four simultaneous in vivo experimentswith synchronized video collection on one computer. Pinnacle’s Sirenia software

Product

Item #

automatically synchronizes recorded video with EEG/EMG waveforms and1-Camera Synchronized Video System

9000-K1

biosensor activity to provide an accurate visual representation of animal activity

2-Camera Synchronized Video System

9000-K2

3-Camera Synchronized Video System

9000-K3

4-Camera Synchronized Video System

9000-K4®

and behavior. Synchronized video can be ordered as an accessory to new systems
or easily added to any research experiment. The base video package includes a

Each system comes with a quantity of cables and licenses matching
the number of cameras each kit supports.
Cameras are sold separately.

SUHFRQ¿JXUHGFRPSXWHUZLWKDPSOHKDUGGLVNVWRUDJHDOOQHFHVVDU\FDEOHVDQG
a Sirenia® software license key. Two camera options are available with different
optics. Up to four cameras can be added to a single video system.

option 1:
Pinnacle’s

domecamerapackagedome camera kit

includes a camera that mounts above the9000-K9:

cage. Built-in infrared illumination adjuststolighting

conditions

Dome Camera System

9022 - Dome camera with built-in IR source

automatically,

allowing recording in reduced lighting

box camera kit

and complete darkness.

9000-K10:

option 2:

Box Camera System

9056 - Box camera

9058 - Mounting clamp

9057 - IR source

9056-LENS - 4 mm lens

The box camera has improved optics

additional products

and low-light performance, as well asÀH[LELOLW\ LQ PRXQWLQJ RSWLRQV ,W FDQbe mounted above the cage using
Pinnacle’s standard mounting plate,on the cage stand, or to a tripod for

Product

Item #

Variable Focus Lens

9056-VF

Tripod9059

recording at lower angles. A separate,
automatic infrared illumination source
Pinnacle’s video camera setup
highlighting camera options and
PRXQWLQJ
FRQ¿JXUDWLRQV 7KH
independent IR source is not shown.

allows video capture in conditions of
reduced lighting and complete darkness.

KEY FEATURES
Record in color or grayscale and from any angle
Flexible ¿OHVL]HPDQDJHPHQW
Resolution up to 640 x 480 pixels and 30 frames per second
Unrestricted video playback
Record in low light or complete darkness
Synchronized Video with data recordings

16

Integrated video can be added toany

Pinnacle

recordingsystemthrough Sirenia® software.

Video issynchronized within 100 milliseconds
tosimultaneous

biosensor data.

EEG,

EMG,

or

SOFTWARE

sirenia® software

X-Y T

R AC K I N GAll Pinnacle hardware is shipped with our FREE SIRENIA®
vi

de

o
de

ze

d

SOFTWARE PACKAGE. This package features an acquisition

au to m

n

at e
d

or

in

g

ch

systems, camera capture is seamlessly integrated and synchronized

sy n

ro

biosensor, sleep, and seizure recordings. For users of our video

se

c
si
ba

s leeps leep

p ro

ure

®

as i csireniaproeb

software programs that automate the sleep scoring and seizureur

In addition, Pinnacle currently offers two advanced analysis

s ei zizwith biosensor and biopotential data.

scni

platform, as well as basic review and analysis programs for

tio
i va
pr

LGHQWL¿FDWLRQSURFHVVHV Learn more about Sirenia® Sleep Pro anduisition

pr

o

+

bi

o

third-party data streams

bios

pr

o+

bio

acq

Sirenia® Seizure Pro on pages 18-19.

ensor
pro

®

Sirenia supports the integration of third-party data streams
via a National Instruments I/O module. These data can be

COMING SOON

simultaneously recorded along with Pinnacle’s biopotential
and biosensor data.
Product

Item #

National Instruments I/O module

9032

DOWNLOAD SIRENIA® FOR FREE
at pinnaclet.com/sirenia.html

ACQUIREREVIEWN_1

Xk = ɯɩn=0

ANALYZE

Key features
BIOSENSOR 1

All Pinnacle hardware can be recorded
to one platform
Basic seizure and sleep analysis tools
Flexible data management

BIOSENSOR 2

Multiple export capabilities
Record synchronized video with all
Pinnacle recording devices

EEG

I/O functionality
Data consolidation
EMG

Free updates

FULL MOTION VIDEO

Two biosensor traces and EEG/EMG waveforms are recorded simultaneously with full-color video using Sirenia® Acquisition.

17

17

SOFTWARE

sirenia® sleep pro
SIRENIA® SLEEP PROZDVGHVLJQHGVSHFL¿FDOO\ZLWKVOHHSUHVHDUFKHUVLQPLQG,WRIIHUVDXWRPDWHGSRZHUDQDO\VLVVHPLDXWRPDWHG
scoring methods, and advanced tabular and graphical analysis for investigation of sleep data sets. All EEG/EMG and video data sets
UHFRUGHGZLWK3LQQDFOHVRIWZDUHDVZHOODVWKLUGSDUW\(')DQGWH[W¿OHVFDQEHLPSRUWHGIRUDQDO\VLVscoring tools

threshold scoringcluster scoring

Semi-automated scoring:- Cluster- Threshold

Mouse

Customizable scoring keys
Real-time hypnograms
Spectral plots
Heat maps

Threshold scoring uses power spectral analysis to create rule setsbased on adjustable power boundaries in one or more channels
and automatically scores epochs that fall within those boundaries.

Cluster scoring allows rapid scoring of similar epochs based on
power analysis plots. Clusters of epochs can be easily scoredby drawing circles around similar groups and assigning a stage.

AUTOMATED ANALYSISCUSTOMIZABLE PLOTTING

Power analysis

Time comparison plots

Sleep stage andsleep bout analysis

Automatic graphgeneration

Peak frequency analysis

Plot multiple power bands
with hypnogram andother data

Compare user scores

8VHUFRQ¿JXUHG VOHHS VWDJHV DQG VOHHS ERXWV DUHautomatically generated for fast and easy analysis.
Analysis can be broken down into light and darkcycles as well as day-by-day reports.

With customized plotting features, data can beplotted on top of one another (i.e., theta/delta bands)
and zoomed in and out to view detailed changes,
providing a powerful and innovative way to review
and analyze data.

Accuracy of sleep pro software

sirenia® sleep pro

A combination of cluster, threshold, and manual scoring
tools were used by four experienced and novice scorers toseparately score three different mouse data ¿OHV$OO¿OHV

Item #

Sirenia® Sleep Pro Analysis Software

9035

ZHUHFRPSDUHGWRH[SHUWKDQGVFRUHGGDWD¿OHV7KHRYHUDOOThe software package can be installed on multiple computers, though

DYHUDJH DJUHHPHQW RI WKH IRXU VFRUHUV IRU DOO WKH ¿OHV DV

each seat is limited to one computer running the program at a time.

compared to the expert is shown below.

Purchase includes one year of free upgrades. Contact Pinnacle forSleep State

additional package options.

Wake

89.7%

NREM

94.7%

REM

80.2%
0

20

40

60

80

100

Percentage Correct Compared to Expert

18

Product

30-DAY

E
FTRRE
L
IA

Download the 30-day free software trials at
www.pinnaclet.com/sleepPRO.html andwww.pinnaclet.com/seizurePRO.html.

Contact a Pinnacle representative for your key:(785) 832-8866 or sales@pinnaclet.com

SOFTWARE

sirenia® seizure pro
The SIRENIA® SEIZURE PRODGYDQFHGDQDO\VLVSDFNDJHSURYLGHVDSODWIRUPIRUTXLFNO\LGHQWLI\LQJDQGDQDO\]LQJXVHUGH¿QHGVHL]XUH
HYHQWVRYHUDJLYHQWLPHSHULRG2QFHYHUL¿HGHYHQWVDUHORJJHGLQDGDWDEDVHDQG((*(0*GDWDDUHDXWRPDWLFDOO\DQDO\]HGDQG
VWRUHGWRFUHDWHFXVWRPL]DEOHUHSRUWVDQGJUDSKVRIDJJUHJDWHVHL]XUHGDWD7KLUGSDUW\(')¿OHVFDQEHLPSRUWHGIRUDQDO\VLV

identification and logging tools
3RVVLEOH VHL]XUH HYHQWV DUH LGHQWL¿HG DQG PDUNHG E\ XVHUFRQ¿JXUHG
parameters, including Root Mean Square (RMS) power, line length, andIUHTXHQF\ UDQJH 8VHUV FDQ GH¿QH EDVHOLQH RU VHL]XUH SDUDPHWHUV IRUindividual animals by highlighting a portion of data. The software scans the
HQWLUH¿OHDQGDQQRWDWHVGDWDPDWFKLQJWKHGH¿QHGSDUDPHWHUV8VHUVUHYLHZDQG FRQ¿UP VHL]XUH HYHQWV DGMXVW WKH DQDO\VLV ZLQGRZ LI QHFHVVDU\  DQGlog EEG/EMG data for advanced analysis. Spectral plots and heat maps
DUH DYDLODEOH WR FRQ¿UP VHL]XUH HYHQWV 5DFLQH¶V VFDOH UDWLQJV VHL]XUHFODVVL¿FDWLRQDQGQRWHVFDQEHDGGHGWRDQ\ORJJHGHYHQWIRUIXWXUHUHSRUWLQJ

automated analysis

customizable graphing

When automated analysis is performed on stored EEG/EMG

Customizable plots and graphs provide users a powerful and

GDWD WKH FKDUDFWHULVWLFV RI HDFK VHL]XUH HYHQW DUH TXDQWL¿HG

innovative way to review and analyze seizure data. Powerover time. The following seizure characteristics are included in

VSHFWUD EDQGV VSHFWURJUDPV DQG WLPHOLQHV RI XVHUGH¿QHG

the analysis:

seizure characteristics are generated using these tools.

Peak frequency

Time from last seizure

Seizure duration

Line length

Percentages of power
frequency bands

Peak voltage

Allcharts and graphs can be easily saved as high-resolution
images for use in publications and presentations.

EEG 1 FULL

All computed data can be exported for use in external
spreadsheet programs. In addition, EEG/EMG data with video
FDQ EH FRQVROLGDWHG LQ XVHUGH¿QHG WLPH VHJPHQWV DURXQG

EEG 1 GAMMAPDUNHGVHL]XUHHYHQWVIRUVPDOOHU¿OHPDQDJHPHQW3OD\EDFN
of EEG/EMG data with synchronized video can be exported as
DQ$9,¿OHIRUXVHLQSXEOLFDWLRQVDQGSUHVHQWDWLRQVEEG 1 LINE LENGTH

Accuracy of sEIZURE detection
Seizure detection was performed using RMS power and line length
VHSDUDWHO\ RQ ¿YH LQGLYLGXDO mousedata ¿OHV $OO ¿OHV ZHUH

sirenia® seizure pro

FRPSDUHG WR DQ H[SHUW KDQGVFRUHU¶V ¿OHV $JUHHPHQW RI WKH WZRdetection methods as compared to the expert is shown below.

Product®

Sirenia Seizure Pro Analysis Software

Item #
9037# of Seizure
Events Marked

Accuracy vs.
Expert Scorer# of False Positives

Expert Scorer

21

N/A

N/A

each seat is limited to one computer running the program at a time.

RMS Power

23

100%

2

Purchase includes one year of free upgrades. Contact Pinnacle forLine Length

21

100%

0

The software package can be installed on multiple computers, thoughadditional package options.

19

Data courtesy of Drs. Philip Haydon and Jerome Clasadonte (Expert Scorer)
Tufts University School of Medicine, Department of Neuroscience

19

FAST SCAN CYCLIC VOLTAMMETRY SYSTEM
Pinnacle’s FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM is robust, turn-

NOR
EP

FSCV
HRINE
EP
IN

DOPAMINE

STRESS RESPONSE

NH\DQGVSHFL¿FDOO\GHVLJQHGWRVLPSOLI\WKHPHDVXUHPHQWRIFDWHFKRODPLQHV LHCIRCADIAN RHYTHMS

dopamine, norepinephrine, and serotonin). It functions by rapidly cycling a voltage

AROUSAL

ENERGYDFURVVDQLPSODQWHGFDUERQ¿EHUVHQVRUDQGPHDVXULQJWKHUHVXOWDQWFXUUHQW%RWK

DEPRESSION

WELL-BEING
SLEEP

SE

R O TO N I N

400 nM DopamineRed

sweep specs

synchronized
video

-0.4

V

excellent temporal
resolution

eep

LEARNING

APPETITE

software.

on

1.1

good spatial
resolution

V

Oxi

dat

-0.4

turn-keysystemadvantages

ucti

V

V sw

REINFORCEMENT

SOCIAL RANK

for controlling an external stimulus. The system is shipped with Pinnacle’s 8500

FSC

REWARDATTENTION

MOOD

the tethered system for mice and the wireless system for rats have built-in support

PLEASURE

FOCUS

FLIGHT OR FIGHT

ion

Voltage span:-1.1 V to +1.3 V

Range:

250 - 400 V/s

Sweeps/second:

5 - 10

Points/sweep:

1000

Free
Softwarebuilt-in stimulus
support
3LQQDFOHRIIHUVDFRPSOHWHOLQHRIFDUERQ¿EHUVHQVRUV
that have been optimized for FSCV applications.

TIM

E

see pages 4-5 for more Information

software features
The 8500 software supports traditional,
short recording paradigms (recordings of
two minutes or less) as well as longer-term
recordings using an extended, continuousmode.
Background subtraction
Heat maps
3D visualization
8VHUVHOHFWDEOH¿OWHUVAnimated voltammograms
Data export

20

A screenshot of Pinnacle’s 8500 software with integrated video.

FSCVTETHERED SYSTEM FOR MICEThe TETHERED FSCV SYSTEM allows researchers to harness the powerful genetics of the mousemodel to address underlying mechanisms of biogenic neurotransmitter release and function. A
headmounted FSCV board sends signals through a low-torque commutator to an interface boxthat streams data to the host PC.

HARDWARE KITS
8500-K1:

accessory KITSFSCV Mouse System (tethered)

8500-K3:

FSCV Accessories for Mice (tethered)

8408 - Mouse commutator with 18” mounting plate7033 - Bio-only headmount (4)8254 - 23-gauge needle (4)8503 - Tethered FSCV headstage

7036 - Clamp rod

8509 - 100K test load

8504 - FSCV interface box

7037 - Probe clamp accessory

8510 - 100M test load

Cables for one animal, software, and manuals arealso included.

7039 - Allen wrench

9005 - 7-port powered USB hub

8212 - 0.12” screws (pkg. of 8)9033 - NiMH batteries (pkg. of 4)8241-F - Screwdriver for mouse screws

9034 - Battery charger

Pinnacle’s tethered FSCV system.

key features
sweep rate: 10 sweeps/second

headmounted amplification

1000 points/sweep

low-torque swivel

calibration kit
Product

Item #FSCV Calibration Kit*

8500-K5

wireless SYSTEM FOR rats

* Used for mouse and rat systems

The WIRELESS RAT SYSTEM USES BLUETOOTH® to transmit data. The system resides in a headmounted enclosure and wirelesslytransmits data to a computer. The battery is easily accessible and readily exchangeable in real-time to support extended recordings. The
system is ideal for mazes and enclosed environments, such as metabolic and behavioral chambers.

HARDWARE KITS
8500-K2:

accessory KITS
8500-K4:FSCV Accessories for Rats (wireless)7036 - Clamp rod

8506 - Battery with cover7038 - Probe clamp accessory

8507 - Rat Hat top

7039 - Allen wrench

8508 - Rat Hat bottom (4)8111 - 1/8” screws (pkg. of 12)8509 - 100K test load

8112 - Drill bit

8510 - 100M test load

8147-A - Hex screwdriver

9024 - Battery charger

FSCV Rat System (wireless)

8501 - Wireless FSCV board
8502 - Bluetooth® dongle
9052 - USB extension cable
Software and manuals are also included.

Pinnacle’s wireless FSCV system.

8241-S - Screwdriver for 1/8” screws

key features
sweep rate: 5 sweeps/second

bluetooth transmission

Ag/AgCl reference electrodes

1000 points/sweep

record from multiple animals within the same room

are sold separately.

disposable

items

21

&DUERQ¿EHUVHQVRUVDQG®

For Mice

For Rats

Item #

Product

Item #

Product7033

Bio-only headmount8111

1/8” screws (pkg. of 12)

8212

0.12” screws (pkg. of 8)

8112

Drill bit

8254

23-gauge needle

8506

Battery with cover

9033

NiMH batteries (pkg. of 4)8508

Rat Hat bottom

21

ADDITIONAL PRODUCTS

animal housing
CIRCULAR CAGES AND CAGE ACCESSORIES are designed toVivarium Products

allow rodents to freely move around a cage while connected to a

Product

Item #

tether. All cages are made of ¼” clear acrylic and are suitable forCage lid for mice

8265-Muse with most commercial cage washing equipment. Multiple sizes

Cage lid for rats

8265-Rare available.

Cage extender for mice

8228-4ex

Cage extender for rats

8238-4ex

Cage for mice (10” diameter, 8” tall)

8228

Cage for rats (12” diameter, 12” tall)

8238

Cage lids allow for additional containment ofCage for rats (14” diameter, 12” tall)

8273

active animals. The design provides security

Cage stand

9009

CAGE LID

without compromising natural cable movement.
Lids can be easily removed for cleaning andfeeding.

CAGE EXTENDER

Increase the height of our standard cages using
FDJHH[WHQGHUV7KHH[WHQGHUV¿WVQXJO\RQWRS
of Pinnacle’s cages and add 4” (per extension)
to cage height.

CAGE
Pinnacle’s cages are ideal for rodent researchbecause their circular design allows the animal
to move freely around the circumference of thecage without creating too much slack in thecable when the animal rests near cage center.
The open top makes cleaning and feedingeasy and accessible. One water bottle, whichattaches to the side of the cage, is included witheach purchase.

CAGE STAND

Pinnacle’s stands accommodate a single
animal cage and mounting plate. The standard
stand’s split design makes it compatible withboth mouse and rat cages and all of Pinnacle’s
tethered recording systems. It is adjustable to24” tall.

CUSTOM FOOD HOPPER

NEED EXTRAS?

Cages with an integrated, external foodhopper are also available. Contact a Pinnacle

22

Product

Item #

Water bottle with holder

8251

14” mounting plate8258

18” mounting plate8426

representative for additional information.

ADDITIONAL PRODUCTS

miscellaneous items
Handheld isolated potentiostat
This general purpose, wireless, one-channel potentiostat can be used

Key features

for a wide range of amperometric systems, including lab-on-chip andbiosensors. Its isolated design makes it uniquely suited for in-channel
detection in capillary electrophoresis systems. The system is powered byPortable

Bluetooth® transmission

Sample rate: 13 Hz

Transmission range: 7 m

Bias range: 0 - 4 V

Current range: 80 uA

Resolution: 24 bits

Battery life: ~100 hrs(continuous use)a standard lithium 9V battery and uses Smart Bluetooth® telemetry to a
USB dongle for reliable data transfer. The potentiostat is fully supported
by Pinnacle’s Sirenia® software suite.

Product

Kit #Handheld Isolated Potentiostat

9000-K7*

COMMUTATORANALOG ADAPTERMASTER CLOCK

Pinnacle’s mouse commutators are ideal for use inAnalog adapters are excellent solutions for researchers

studies that require a low-torque electrical swivel.

ZKR KDYH H[LVWLQJ DPSOL¿FDWLRQDFTXLVLWLRQ V\VWHPVThe master clock is a central timing source for very

The lightweight nature of our commutators provides

but require the noise reduction provided by Pinnacle’s

long-term experiments that require high-precisiona platform for transferring electrical signals without

KHDGPRXQWHG SUHDPSOL¿HUV DQG FRPPXWDWRUV 7KHsynchronization of multiple devices. The unit connects

VLJQL¿FDQWO\DOWHULQJWKHEHKDYLRURIWKHDQLPDO&XVWRP

adapters can be used to connect Pinnacle mouse anddirectly to other devices via three TTL ports andadapters can be ordered for integration into existing

UDWSUHDPSOL¿HUVZLWKWKLUGSDUW\GDWDFROOHFWLRQV\VWHPVcommunicates with data acquisition software using athird-party cabling/connection schemes. Available for

USB cable. Because it eliminates latency, the mastermice only.

clock is perfect for optogenetics and behavioral studies.

Product

Kit #Product

Item #

3-Channel Analog Adapter

8242-K*

Six-pin commutator

8204

Product

Kit #4-Channel Analog Adapter

8442-K*

Ten-pin commutator

8408

Master Clock

9000-K8** Cables, software, and manuals are included in all kits.

learn more
Pinnacle’s products are used daily to advance research at academic institutions, research hospitals, contract research organizations, and pharmaceuticalcompanies across the world. Learn more about how current customers are using our products by visiting the Info Center on our website at www.pinnaclet.
com/info-center.html.

publications

posters

linkedin forum

Many Pinnacle customers regularly publish their

Missed a conference? Visit us online to review

Sign up for our LinkedIn group in order to

VFLHQWL¿F ZRUN LQ SHHUUHYLHZHG MRXUQDOV 7KLV

recent posters presented by Pinnacle and our

network and share information with otherspace is dedicated to sharing our customers’

customers at Neuroscience, AES, Sleep, andscientists,

research with other users of Pinnacle products

other major conferences.

and executives who are shaping theconferences

distributors

3LQQDFOH7HFKQRORJ\UHJXODUO\DWWHQGVVFLHQWL¿F

Pinnacle

conferences and meetings hosted both in theour products available to an international

United States and internationally. Please stop bycustomer base by teaming with a growing list

our booth at upcoming conferences to chat withof distributors from across the globe. Visit oura representative about how our cutting-edge

website at www.pinnaclet.com/distributors.html

tools can improve and simplify your research.

for an updated list.

ourwebsitetechnicians,

ELRPHGLFDOUHVHDUFK¿HOG

and systems.

Visitresearchers,atTechnology

is

proud

to

make

www.pinnaclet.com/

conferences.html for a complete list of events.

23

23

elevate your research

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RESULTSPINNACLE TECHNOLOGY, INC. | 2721 OREGON STREET | LAWRENCE, KS 66046

24

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