COINS Seminar #21“Polymer coatings for dynamic adjustment of specific andnon-specific interactions for cell culture”

CNRS Researcher, Emmanuelle MARIEENS - Chemistry Department, Biophysical Chemistry Group(Paris, France)
日時：2016 年 10 月 25 日（火）15:00～16:00（受付開始 14:30）
会場：ナノ医療イノベーションセンター（iCONM）3 階 3001 号室
定員：30 名
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Email: jimukyoku-coins@kawasaki-net.ne.jp—Abstract—
Tools providing in situ control of the formation of patterns on surfaces are highly in demand forsensors, cell biology, and tissue engineering, to orient cell migration, proliferation. These surfaces
must initially be repellent to cells and proteins (stealthy), and become prone to cell adhesion, orspecific binding upon non-toxic stimulation. Reaching this goal is however technically challenging,and needs usually to combine advanced microfabrication with surface chemistry. To make such
patterning more versatile, we tailored copolymer chains that spontaneously bind from their water
solutions onto most usual materials (e.g. petri dish, glass plates, etc…), form biocompatible densehydrophilic brushes, and afford stimuli-triggered patterning. Conversion of the properties ofpatches was achieved by either exposing the surface to solutions of bioorthogonally reactive small
molecules, or by temperature variation.
Poly(L-Lysine)-graft-poly(ethyleneglycol) copolymers (PLL-g-PEG) are cationic chains successfully used to coat, uponphysisorption most anionic surfaces. In aqueous solutions, the resulting monolayer of PEG imparts the substrates with ahigh resistance to adsorption of proteins. Patterns were formed by burning this layer with deep UV, and subsequent(re)coating of the uncoated regions with another grafted PLL derivative. This approach allowed photopatterning at
micrometer resolutions for advanced spatial control. Using PEG grafts that carried azido groups, we obtained reactive
patterns surrounded by a non-reactive PEGylated surface. Attachment of molecules, or proteins was triggered on demandon the initially repellent azido-presenting pattern, by copper-free “click” addition with alcyne-attached fluorophores, biotin,or peptides. The reaction with RGD peptide was for instance implemented in the presence of cells floating above the surface,
thereby triggering with high temporal resolution cell adhesion, or migration. To allow a dynamic on/off control of theinteractions with the patterns, we used alternatively PLL carrying temperature-responsive grafts that undergo a transition
from well-hydrated, protein-repellent state to hydrophobic state above 32°C. These PLL-PNIPAM copolymers allowed usto thermo modulate cell adhesion.
*主催: JST COI プログラム
スマートライフケア社会への変革を先導するものづくりオープンイノベーション拠点 (COINS)*座長: Horacio Cabral（オラシオ カブラル）
東京大学大学院工学系研究科バイオエンジニアリング専攻 准教授＊問い合わせ先：川崎市産業振興財団 COINS 支援事務局 TEL: 044-589-5785
E-mail: jimukyoku-coins@kawasaki-net.ne.jp Web: http://coins.kawasaki-net.ne.jp/

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