NIPPON Genetics EUROPE GmbHwww.nippongenetics.eu
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Source: Bacillus globigii

Restriction Enzyme
Bgl I
Cat.#FG-BglISize
2,000 units

Reaction conditions
1X FastGene® Buffer III 37℃
1X FastGene® FastCut Buffer, 37℃
Conc.
5 units/μl

Store at -20℃

FastGene® FastCut Buffer
FastGene® restriction enzyme can cut substrate DNA in 5-15
with FastGene® FastCut Buffer.

1X FastGene® Buffer III
Supplied with: 10X FastGene® Buffer III(FG-REB3)
10X FastGene® FastCut Buffer (FG-REBHF)
6X DNA Loading Buffer
Sterile water

50 mM Tris-HCl (pH 7.9 at 25℃)
100 mM NaCl
10 mM MgCl2
100 μg/ml BSA

Recognition site

Unit definition
One unit is defined as the amount of enzyme required forcomplete digestion of 1 μg bacteriophage λ at 37℃ for 1 hr in
50 μl reaction mixtures.

Quality control
For Research Use Only. Not for use in diagnostic procedures.

Dilution buffer:

FastGene® Diluent B.

NIPPON Genetics EUROPE GmbHHeat
Inactivationwww.nippongenetics.eu- Unit definition assay- Overdigestion assay- Endonuclease assay- Extreme pure assay

Standard reaction condition- Normal protocol
Component

Final Conc.

VolumeBgl
I can be inactivated at 65℃ for 20 min.
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Substrate DNA

1 μg

X μl

Methylation sensitivity

10X FastGene® Buffer III

1X

5 μl

Bgl I

5 unitdam methylation: Not sensitive
dcm methylation: Not sensitiveCpG methylation: Conditionally sensitive

Restriction Enzyme
Prolonged
Bgl I incubation

A minimum amount of enzyme required to digest 1 μg substrate
DNA for 16 hr; 0.13 U.
Cat.#SizeConc.
® Buffers
Relative
FG-BglIactivity in FastGene
2,000 units
5 units/μl®
FastGene Buffer I:
75%® Buffer
Store at-20℃ II:
FastGene
75%®
100%
FastGene Buffer III:®
50%
FastGene Buffer IV:®
Suppliedwith:
10X
FastGene(FG-REB3)®
FastGene FastCut Buffer:
100%III
10X FastGene® FastCut Buffer (FG-REBHF)
Note
6X DNA Loading Buffer
Five-fold higher activity
can beobtained at pH 9.5 than pH 7.5.
Sterilewater
Cleavage of supercoiled DNA requires more (10 fold) enzyme. The
sequences of sticky ends produced by this enzyme are unique to eachsite,Recognition
thus, this enzymesite can be conveniently used for unique cloning
strategies; replacement of a wild-type fragment with a mutant one in aplasmid. Cleavage of mammalian genomic DNA can be blocked by
CpG methylation that partially overlaps its recognition sequence. It issensitive to impurities in DNA and buffer condition.

Source: Bacillus globigii

Reactionconditions
Sterile water® Buffer III 37℃
1X FastGene→ Incubate
at 37℃ for 1 hr
1X FastGene® FastCut Buffer, 37℃- Fast protocol

FastGene®Component
FastCut Buffer

Final Conc.
VolumeFastGene® restriction enzyme can cut substrate DNA in 5-15
Substrate DNA
1μg
X μl
with FastGene® FastCut Buffer.
®
10X FastGene FastCut Buffer
1X5 μl® Buffer III
1X BglFastGeneI

50 mM Tris-HCl (pH 7.9 at 25℃)
water100SterilemM NaCl

5 unit1 μl
up to 50 μl→ Incubate
10 mM
MgCl2 at 37℃ for 15 min
100 μg/ml BSA※ We recommend 5-10 units of enzyme per µg DNA and 10units for genomic DNA in a 1 h digest.
Unit20definition

One unit is defined as the amount of enzyme required forcomplete digestion of 1 µg bacteriophage λ at 37℃ for 1 hr in
50 μl reaction mixtures.

Quality control
For Research Use Only. Not for use in diagnostic procedures.

1 μlup to 50 μl- Unit definition assay- Overdigestion assay- Endonuclease assay- Extreme pure assay