The Joint Meeting of the 3rd Symposium on “Oral Health
Science “ and the 3rd symposium on “Dental andCraniofacial Morphogenesis and Tissue Regeneration”

PROGRAM

January 26, 2008
Fukuoka Recent Hotel, Fukuoka, Japan.

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PROGRAM
Opening Remark
9:15-9:20

Hiroshi Nakanishi, Project Leader

9:20- 9:30

Akifumi Akamine , Dean of Kyushu University Faculty of Dental Science

Invited Lecutures<Chair persons: Kazuaki Nonaka & Hidetaka Sakai>
9:30- 10:10 Invited lecture-1“Development of a Bioengineered Organ Germ Method for Future Tooth
Regenerative Therapy”
Takashi Tsuji
Faculty of Industrial Science and Technology, Tokyo University ofScience, Tissue Engineering Research Center, Tokyo University ofScience, Japan
10:10-10:50 Invited lecture-2“BMP-signaling in craniofacial development”
James F. Martin
Texas A&M Health Science Center, Institute of Biosciences andTechnology, Huston, TX, USA.
Short break
11:10-11:50

Invited lecture-3
Akihiro Umezawa
Department of Reproductive Biology, National Institute for Child
Health and Development, Tokyo, Japan.

Lunch
12:00-13:00<Chair person: Seiji Nakamura>
13:00-13:40

Invited lecture-4

13:40-14:20

Invited lecture-5“Anti-Aging Medicine for Dentistry in Japan –Seeking innovative oral
health care –”Ichiro Saito
Department of Pathology, Tsurumi University School of Dental Medicine,
Yokohama, Japan.

Short break
Presentations of Annual Research Reports on the Projects
Part 1<Chair person: Toshio Kukita>
14:40-15:05“Roles of thymosin beta 4 and nucleolin in the developing tooth germ of themouse lower first molar”
Ieyoshi Kobayashi, Tamotsu Kiyoshima, Hideaki Sakai

15:05-15:30“Protogenin, a new member of the immunoglobulin superfamily, is implicatedin the development of mouse lower first molar”
Tamotsu Kiyoshima, Ieyoshi Kobayashi, Hideaki Sakai

15:30-15:55“TGF- signaling plays a role in osteo-chondroprogenitor cell lineage
determination during mandible development ”
Kyoko Oka
Section of Pediatric Dentistry, Division of Oral Health, Growth andDevelopment, Kyushu University Hospital

15:55-16:20“A possible suppressive role of galectin-3 in osteoclastic bone destruction
accompanying adjuvant-induced arthritis in rats”
Li, Y., Teramachi, J., Nagata, K., Wu, Z., Kukita, A., Akamine, A.,
Kukita, T.

16:20-16:45“Inhibition of the inflammation-induced bone destruction throughmodulating phagocytes functions by phosphatidylserine-liposomes”
Wu, Z., Ma, H.M., Kukita, T. and Nakanishi, H.

Short breakPart 2
<Chair person: Dr. Yoshihisa Yamashita>
17:00-17:25“Comparative Studies of the peri-implant epithelium of rat gingivae
betweenplatform-switched c andnon-switched c implantsystem”
Ikiru Atsuta
Section of Removable Prosthodontics, Division of Oral Rehabilitation,
Faculty of Dental Science Kyushu University, Japan.
17:25-17:50“An attempt to make a new method of definitive diagnosis in dry mouth
Masafumi Masafumi”
Section of Oral and Maxillofacial Oncology, Division of Maxillofacial
Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu
University, Fukuoka, Japan.

17:50-18:15

Nakamura

18:15-18:40

Shigemura

18:40-19:05“”

Getting Together Party<M.C.: Yuzo Ninomiya, Project Leader>
19:20-21:00
Closing Remarks
20:50-21:00

Kiyoshi Koyano, Associate Director of Kyushu University HospitalDevelopment of a Bioengineered Organ Germ Method
for Future Tooth Regenerative Therapy
Takashi Tsuji
Faculty of Industrial Science and Technology, Tokyo University of Science
Tissue Engineering Research Center, Tokyo University of Science, JAPAN.

The ultimate goal of regenerative therapy is to develop fully functioning bioengineered organs
that can replace lost or damaged organs after disease, injury or aging.

The development of

three-dimensionally reconstructed bioengineered organs from dissociated single cells in vitro
is a goal of this technology.

The “tooth” is a good feasibility study model in regenerative

medicine because cells are easily obtained and even if extracted from an adult for study in thetransplanted model, the process is rarely life-threatening.

To bioengineer ectodermal organs

such as teeth and whisker follicles, we developed a three-dimensional organ-germ culture
method using collagen gel.

The bioengineered tooth germ generates a structurally correct

tooth following both transplantations under a subrenal capsule in vivo and also in in vitroorgan cultures with a high frequency.

By in situ hybridization analysis, it was indicated that

our current tooth germ model reproduced the interaction between epithelial and mesenchymal
cells in early tooth organogenesis.

We additionally generated a tooth cavity by extraction ofa mandibular incisor in adult mice and find that a complete tooth structure, showing
penetration of nerve fibers, can be successfully developed following the engraftment ofbioengineered primordium isolated from cultured bioengineered tooth germ.

Our model

provides a substantial advance in the development of bioengineered organ replacement
strategies and regenerative therapies.

In this symposium, I would like to discuss why andexplain how we are trying with the hurdles of “tooth regenerative therapy” approached fromorganogenesis.

Figure: Representative phase contrast images
showing a bioengineered tooth germ (left) andEM

teeth (right) developed in a subrenal capsule for14 days. E: epithelial cells, M: mesenchymal cells.

BMP-signaling in craniofacial development
James F. Martin
Texas A&M Health Science Center, Institute of Biosciences and Technology, Huston, TX, USA.

The calvarium forms the skull vault that is necessary for brain protection. We investingatedBone morphogenetic prottein4 (Bmp4) function in calvarial development using conditional
gene targeting in mice. Using a Bmp4 conditional null allele and the Wnt1cre transgene toinactivate Bmp4 in cranial neural crest (CNC), we found that Bmp4 deficient embryos hadparietal foramina (PFM, OMIM #168500), a defect that involves failed fontanelle closure.
Bmp4 was required to maintain cell proliferation of undifferentiated head mesenchyme. Msx1
and Msx2 expression was diminished while Twist1 expression was unchanged in Bmp4 mutantembryos. We investigated the genetic relationship between Twist and Bmp4-signaling using aconditional null allele of Twist1. Our data indicate a strong gentic interaction between Twist1
and Bmp4 indicating that Twist 1 is a component of the Bmp-signaling pathway.

Anti-Aging Medicine for Dentistry in Japan– Seeking innovative oral health care –Ichiro Saito
Department of Pathology, Tsurumi University School of Dental Medicine, Yokohama, JAPAN

Anti-aging medicine is defined as the medicine which makes maintaining health and youth
possible while growing older. The aim of this innovative medicine is not simply to extend thelife span, but to prevent age-associated physical and mental decline and maintain a high qualityof life thus enabling continued social productivity. Instruction on health enhancement andtherapy based on anti-aging health care, namely anti-aging medicine, is a new preventive
method and a concrete effort to achieve the Ministry of Health, Labor and Welfare’s goal of its“Healthy Japan 21” campaign to promote better health in the 21st century. It is also hoped that
evidence-based anti-aging dental medicine will be implemented in the dental medicine field.
In the year 2050 almost 40% of Japan’s population will be over the age of 65 years. The
decline in the incidence of dental caries and periodontal disease means that the time for atransformation in conventional dental medicine is imminent and it will be essential tointroduce anti-aging medicine to the dental field in order to create a framework for innovative
oral health care.
Saliva has growth factors and antibacterial agents important for biological homeostasis,and various other substances vital for maintaining general health. It is therefore an essential
theme for the implementation of anti-aging medicine and various methods are proposed tostimulate salivary secretion. Initial evidence suggests the possibility of clinical application ofanti-oxidant supplements to treat oxidative-stress related aging diseases, including age-related
dry mouth disorders.
The dentistry field also has a deep involvement in heavy-metal contamination. It is
becoming evident the influence on aging which dental materials used in dental treatment anddentures have. The medical field is also hoping that dentists will make active efforts todiagnose and improve these conditions. It is clear that the anti-aging medicine region in which
dentistry is involved is very diverse. Close cooperation between the dentistry and medical
fields will make it possible to implement the above methods thus resulting in a high level ofanti-aging medicine.