Research Activity Report
Supported by “Leading Graduate Program in Primatology and Wildlife Science”(Please be sure to submit this report after the trip that supported by PWS.)

2014. 06. 09
Affiliation/Position

National Institute of Amazonian Research/INPA

NameWaleska Gravena

1. Country/location of visit
Japan, Kyoto

2. Research project
Study on the microbiota metagenomics of the Japanese monkey

3. Date (departing from/returning to Japan)
2014. 05. 29 – 2014. 06. 06 (08 days)

4. Main host researcher and affiliation
Dr. Agata, Professor at WRC Institute, Kyoto University

5. Progress and results of your research/activity (You can attach extra pages if needed)
Please insert one or more pictures (to be publicly released). Below each picture, please provide a brief description.

During my stay in Kyoto, I conducted research in genomics using feces of monkeys collected on the island of
Yakushima and Koshima. This study aimed to compare the constitution of the intestinal microbiota of monkeys ofdifferent groups and especially between different islands in order to try to estimate from the establishment of themicrobiota if the animals have the same diet in different localities.
For this, we use collected samples to extract DNA and amplify the 16S gene region, considered a barcode in bacteria
and it is mainly used for species identification. The genome training course aimed to teach us the techniques of sample
preparation to use the next generation sequencing platforms, and especially as the data obtained by this new equipment
should be analyzed. So, after preparation of the samples, they were injected into a flowcell used for sequencing in
MiSeq equipment (Illumina).
Several teachers gave lectures and taught us how to analyze the data obtained by next-generation sequencing in new
programs developed by them and using the R platform.
After the course, we analyzed data obtained with our samples and prepare a poster (Figure 1) with our results for data
presentation at the 3rd International Seminar on Biodiversity and Evolution (Figure 2), where we also had theopportunity to know other works in the area of genomics performed by fellow students.
I am currently working on a report based on these results for publication.
In Brazil I am working with genetics for 14 years, but never had the opportunity to undertake work in the area ofgenomics. This course was very important for my career because I intend to use the techniques I learned in Japan insimilar studies with samples from the Amazon.

6. Others

Submit to：report@wildlife-science.org

2014.05.27 version

Research Activity Report
Supported by “Leading Graduate Program in Primatology and Wildlife Science”(Please be sure to submit this report after the trip that supported by PWS.)

Figure 1 – Poster with the results of the Genome Training Course presented in the International Seminar.

Submit to：report@wildlife-science.org

2014.05.27 version

Research Activity Report
Supported by “Leading Graduate Program in Primatology and Wildlife Science”(Please be sure to submit this report after the trip that supported by PWS.)

Figure 2 – Poster presentation at the 3rd International Seminar on Biodiversity and Evolution.

Submit to：report@wildlife-science.org

2014.05.27 version