Research Activity Report
Supported by “Leading Graduate Program in Primatology and Wildlife Science”
2014. 06, 03
Affiliation/Position

Science Officer, School of Biological Sciences, Universiti Sains Malaysia

Name

Mohd Abdul Muin Md Akil

1. Country/location of visit
Kyoto University, Japan.

2. Research project
Genome Training Course

3. Date (departing from/returning to Japan)
2014.05.29 – 2014.06.05

4. Main host researcher and affiliation
Professor at Shiro Koshima, Wildlife Research Centre, Kyoto University

5. Progress and results of your research/activity

Genome training course 2014 was held for 8 days. This training requires each personnel to choose one among a few
titles of genome course prepared by the instructor, in accordance to their level of experience with genome research. Thecourse namely are:Next Generation Sequencing (Medium to expert level)
1. Monkey host genome
2. Insect genome
3. Plant Genome
4. Gut microbiota
Sanger Direct Sequencing (Beginner level)
1. Plant DNA
2. Insect DNA
During this training course, I have chose to do Insect Genome using Next Generation Sequencing. Next generation
sequencing method is new to me, so the learning process is quite tough, as we need to learn and analyze many samples
in such a short time.
Samples used were the DNA samples taken during the Yakushima Island fieldwork. For insects genome, we used thefecal samples stored in RNA later buffer. We also added a few samples from leopard feces and giant oarfish gut
samples. In total, 21 samples were used.
DNA extraction and purification from fecal samples were done using QiaAmp DNA Stool mini kit and a standard
protocol prepared by the instructor. Extracted DNA was quantified using NanoDrop and Qubit dsDNA HS Assay Kit.
Prior to the sequencing, Agilent Tapestation was used to measure the quality of the DNA in each sample. Samples withgood results will be put into Illumina MiSeq next generation sequencer.
After the sequencing result is out, Claident software and R statistical package were used to analyze and summarize theresults.
For insect genome, the result is not so good where we only find one good match for the insect DNA obtained from theClaident database. As for the matching with Insect DNA group result, we also only get one good result with 99.7%
match.
In conclusion, I really enjoy this training and I learnt many new techniques and methods in DNA extractions andanalysis. Hopefully I can use it for my future research and study.
Submit to：report@wildlife-science.org

2014.05.27 version

Research Activity Report
Supported by “Leading Graduate Program in Primatology and Wildlife Science”
6. Others

Submit to：report@wildlife-science.org

2014.05.27 version

Research Activity Report
Supported by “Leading Graduate Program in Primatology and Wildlife Science”

I would like to extend my gratitude especially to Prof. Shiro Kohshima and Wildlife ResearchCentre for inviting me to participate in this training. I also would like to thank all of the lecturers
and mentors involved with this training. Your valuable guidance and knowledge sharing during thistraining will always remain in my mind and forever be appreciated. To my supervisor, AssociateProf. Shahrul Anuar, thanks a lot for choosing me as one of the representative for Universiti Sains
Malaysia. The knowledge and experience gained from this training will be shared and used tofurther enlighten our knowledge on wildlife research and conservation.

Thank you.

Submit to：report@wildlife-science.org

2014.05.27 version