Safe Handling Guide forRadioactive Reagents:
For Those Using Radioisotopes for the First TimeEven more detailed information
is published in the web version(only Japanese).

RIkkumaOverview of Handling Radioactive Reagents
We have prepared this guide to help researchers who will handle radioactive reagents to safely
and smoothly conduct experiments. Each item is explained in even more detail in J-RAM ( acomprehensive information site for radioactive reagents) operated by the Japan Radioisotope
Association as a web version.
Please access J-RAM from the QR code on the right o the following
URL: https://j-ram.org/.

Overview of Handling Radioactive ReagentsFirst, let’s get an overview of handling radioactive reagents and then learn about eachmatter on the subsequent pages.

1. Learn about the Characteristics of Radioactive Reagents (p. 3 to p. 8)
1) Basic Knowledge

We explain about radiation, exposure, protection and the main Radioisotopes.
It is easy to confuse radiation, radioactive and Radioisotopes, so we carefully
explain these.

2. Make an Experiment Plan (p. 9)2) Preparations Before Entering a Controlled Area

Make an experiment plan and then check with the Radiation Safety Manager whether
you can handle the radioactive reagent you wish to use in the controlled area.

3. Register As a Radiation worker (p. 10)
2) Preparations Before Entering a Controlled Area

To conduct an experiment at a facility, you need to become a Radiation worker atthat facility. Undergo a medical examination and training to become a Radiation
worker.

4. Order the Radioactive Reagents (p. 10)
2) Preparations Before Entering a Controlled Area
Prepare a purchase order and obtain the approval of the Radiation Protection
Supervisor. After that, the Radiation Safety Manager will place an order with theJapan Radioisotope Association to complete it. Please refer to J-RAM for details.

15. Enter the Controlled Area (p. 11)
3) Method of Entering a Controlled Area)

You will conduct your experiment in a laboratory (work room) in a controlled area unlikewith regular experiments. Fill in the entry record book, make advance preparations andcheck the method of entering the controlled area/use room.

6. Receiving the Radioactive Reagents (p. 12)4) Receipt and Storage of Radioactive Reagents

In most cases, radioactive reagents are carried to a designated location (e.g.,a Radioisotope control room). The Radiation Safety Manager will receive the radioactive
reagents. However, the user will have to understand the method of storing them.

7. Start the Experiment (p.13 to 16)5) Handling of Radioactive ReagentsTake the radioactive reagents from the storage room into the laboratory.
It is very important to have the expertise to prevent contamination unlike with regularexperiments.

8. Clean Up the Things You Have Used (p.17 to 19)
6 to 8) Contamination and Disposal

Measure the contamination at the end of your experiment. Return the radioactive
reagents you have finished using to the storage room. Properly dispose of contaminatedequipment. If a laboratory bench is contaminated, contact the Radiation Safety Manager
and take appropriate action.

9. Exit the Controlled Area (p. 20)
9) Method of Exiting a Controlled Area

Wash your hands and then test your body and the articles you will take out of thecontrolled area for contamination in the contamination test room. After that, change yourclothes and return your personal dosimeter. Finally, fill in the entry record book and exit
the controlled area.

Contamination
Inspection RoomRadioactive reagents may bedifferent from normal reagents,
but they are not complicated if
you organize them.

21-1) Basic Knowledge

Features of Radioisotope Experiments(1) Diverse Research Areas Where Radioisotopes Are Used
• It is possible to use Radioisotopes in diverse research areas – from the elucidation of specific life
phenomena to drug discovery research.
• In particular, there has been progress in recent years on imaging technologies using positronemission tomography (PET) and single photon emission computed tomography (SPECT).
Accordingly, the areas in which Radioisotope experiments are used are expanding.

Basic research: Molecular cell biology and biochemistry
Applied research: Medical science, pharmacy, veterinary medicine, nutritional
science and agriculture(2) Lineup of Diverse Radioactive Reagents• Radioisotope can label various compounds. Therefore, there are many product lineups.
This means it is possible to conduct experiments to meet various needs.
• It is also possible to label compounds by themselves using Radioisotopes.
Therefore, there is a wide range of possibilities here.
• It is possible to manufacture desired Radioisotopes with an accelerator.
It is also possible to use these in experiments as standalone Radioisotopes.

Diverse labeling targets: DNA, RNA, peptides, proteins, antibodies, compounds
and cells(3) Chemical Behavior• Chemical behavior is almost the same as the original compound even if labeled.
Therefore, Radioisotopes display strengths in experiments to analyze the dynamic state ofcompounds (e.g., pharmacokinetic analysis).

(4) High Sensitivity• Radioisotope experiments have an extremely high detection sensitivity.
This means it is possible to detect very small amounts of substances.
Accordingly, even a very small dose may be sufficient.

(5) Quantification•

3

Radioisotope experiments are characterized by high quantification.
If you require quantification, you should consider a Radioisotope experiment.

1-2) Basic Knowledge
Main Experiment Techniques Possible Using
Radioisotopes• It is possible to conduct various experiments according to the research purpose and application.
These all feature excellent sensitivity and quantification compared to other techniques.

Experiment

Experiment Overview

Radiometric ligand-binding

Measure the binding of radiolabeled ligands to cells or cell
membranes including the desired receptor.

35S GTP binding

This is a technique(method) to measure the activity of G
protein-coupled receptors (GPCRs).

Radioimmunoassays

Radioimmunoassays (RIAs) use antibodies to detect andquantitate the amount of antigen (analyte) in a sample. It is
possible to quantify proteins(a method of high sensitive protein
quantification) with a principle similar to the ELISA – a nonRadioisotope technique.

Thymidine incorporation

This is a technique(method) to assess(study) cell proliferation.
Assess natural or synthetic compounds that stimulate or inhibit
the proliferation of cells (e.g., lymphocytes).

51Cr release assay

This is a technique(method) to quantify cytotoxicity in tumorsand viral cells(cytolysis) in particular.

DNA and RNA labeling in vitro

Radioisotope labeled nucleic acid probe makes possible todetect nucleic acid and nucleic acid binding proteins withspecific base sequences.

125I Labeling of proteins

Iodize proteins and use them for receptor binding assays.

ADME test using rats and miceThis is a technique(method) to assess absorption, distribution,
metabolism and excretion using Radioisotope labeled
compounds.

Plant Radioisotope imaging

This is a technique(method) to assess the distribution andmovement of chemical elements themselves in plants.

Reference: Fundamental Method Guide of Life-science
Experiment Using Radioisotope• We are currently preparing an experiment guide for beginners
containing the experiment techniques used in the life science field.
(We plan to release this in FY2019.)
Make a second choice with radioactive reagents if you
wish to acquire data with high sensitivity or
quantification or if you are not able to obtain data as
you desired with a fluorescent reagent.

41-3) Basic Knowledge

What Are Radiation and Radioisotopes?
(1) Familiar Radiation• Radiation exists in nature. Humans live in that.
• The feature of radiation is that we cannot feel it with our five senses– we cannot see it, we cannot hear it and it is tasteless and odorless.

(2) Radiation Categories and Their Characteristics• We can classify radiation into α rays, β rays, γ rays and neutron rays.
The experiment characteristics and handling precautions differ depending on the category.
• Radiation has the property of penetrating substances.
The penetration strength and distance differ depending on the category.
Accordingly, the detection methods and protection methods differ.

The features of each are
published in the web
version!

(3) Radioisotopes (Radioisotopes)• Atoms with the same number ofprotons but a different number ofneutrons are said to have a mutualisotopic relationship.
• When the nucleus emits radiationand changes (radioactive decay) inthat, we call this a radioisotope(Radioisotope).
• Radioisotopes cause radioactive decay
and change to an even more stableatom.
• This property is called radioactive. We
use becquerels (Bqs) as the unit for this.

51-4) Basic Knowledge(4) Radioactive Decay• Radioactive decay refers to the phenomenon in which Radioisotopes change into stable
nuclei by emitting radiation.
• The main forms of radioactive decay are α decay, β decay and γ ray emission.
• β decay contains β－ decay, β＋ decay and orbital electron capture.

β decay contains β－ decay,β＋ decay and EC decay!

(6) Radioactive Reagents• Radioactive reagents indicate reagentsfor which some of the atoms of the target
compound have been replaced with
Radioisotopes.
• It is possible to investigate the chemical
behavior (movement and distribution) in thesystem and the chemical reaction processby measuring the radiation emitted fromthese Radioisotopes.

61-5) Basic Knowledge

What Is Exposure?
• When radiation enters the human body, it ionizes and excites the atoms that constitute thebiopolymers.
• If DNA is damaged during this process, it may affect cells as well as organs and the human body.
• If radioactive reagents are handled and protected properly, the effect on the human body isextremely small. Therefore, there is no need for excessive worry.
• Radioisotopes are found outside the body. Receiving radiation from outside the body is called
external exposure while receiving radiation from inside the body is called internal exposure.
DNA severed!

*Exposure from radioactive reagents is about
several μSv at most.

Radioisotope

What Is Radiation Protection?
(1) External Exposure Protection• It is possible to reduce external exposure by paying attention to distance, shielding and time.
Distance

Shielding

Keep away fromRadioisotopes asmuch as possible!

Use shielding materials
between Radioisotopes
and yourself!

Time

Shorten the time you
emit Radioisotopes!

(2) Internal Exposure Protection• Radiation is continuously emitted until discharged outside your body.
Therefore, there is a high possibility of a greater effect on your body.
• The following acts are prohibited inside controlled areas because Radioisotopes may be taken
into your body.
Eating and drinking

7

Smoking

Ｗearing Makeup1-6) Basic Knowledge

Radioisotope Lineup(1) Overview• Radioactive reagents have various characteristics for each category of Radioisotope used for thelabeling.
• It is possible to reduce exposure and safely conduct experiments by handling according to
the characteristics for each Radioisotope.
• We list only a lineup here. We introduce the features in the web version.

(2) Life Science Experiment Applications
3H

14C

32P

35S

51Cr

125I

Half-life

12.3
years

5,700
years

14.3 days

87.5 days

27.7 days

59.4 days

Shielding*Not
necessary

1 cm-thickacrylicplate

1 cm-thickacrylic plate

1 cm-thickacrylic plate

Halved witha 3 mm leadplate

Halved with
0.02 mm lead
foil

Category ofradiation

β⁻

β⁻

β⁻

β⁻

γ and Xrays

γ and X
rays

*Shield to prevent external exposure in regular Radioisotope experiments.

(3) Molecular Imaging Research Applications
11C

13N

15O

18F

67Ga

99ｍTc

111In

201Tl

Half-life

20
minutes

10
minutes2
minutes

110
minutes

3.3
days

6 hours

2.8
days

3days

Experimentapplication

PET

PET

PET

PET

SPECT

SPECT

SPECT

SPECT

Categoryofradiation

β⁺

β⁺

β⁺

β⁺

γ

γ

γ

γ

(4) Cancer Treatment Research Applications
90Y

131I

177Lu

211At

223Ra

225Ac

Half-life

2.7days

8 days

6.7 days

7.2 hours

11.4 days

10 daysCategoryofradiation

β⁻

β⁻

β⁻

α

α

α

8

2-1) Preparations Before Entering a Controlled Area

1. Make an Experiment Plan
(1) Experiment Plan•••

Make an experiment plan and then check with the Radiation Safety Manager whether
you can handle the radioactive reagent you wish to use in the controlled area.
You may not be able to conduct your desired experiment at the facility, so
please consult with the Radiation Safety Manager on the content of yourexperiment.
Please refer to the Basic Life Science Experiment Guide for details of how to makeyour experiment plan. (Released in December 2019.)(2) Controlled Area••

The controlled area is the location where the Radioisotopes are handled.
It refers to the special area established so that regular people cannot enter it indiscriminately.
Radioactive reagents are handled in the work room (hereinafter referred to as the “use room”) within thecontrolled area be provided for in the law .

Controlled area→(Differs depending on the site)*Radioisotope Usage Precautions• You cannot take out from the controlled area and use even small amounts of radioactive reagents.
• However, if use outside the controlled area would be useful (e.g., if using a device installed outside the controlled area orradioisotopes below the exemption level) use outside the controlled area may be permitted by obtaining the permission of the
Nuclear Regulation Authority in advance.

We recommend reading the textbook on the rightif you wish to lean more detailed basic knowledge(only Japanese).
URL:https://www.jrias.or.jp/books/
cat3/cat33/nyumon.html#06

92-2) Preparations Before Entering a Controlled Area

2. Register as a Radiation worker• To conduct an experiment at a facility, you need to become a Radiation worker at that facility.
• Please apply to register with the facility’s Radiation Safety Manager and submit the necessary
documents (e.g., usage plan).

2-1. Medical Examination• Undergo a medical examination before entering the controlled area and for each period
not exceeding one year after entering it.
Oral interview ：exposure history, etc.
Test or medical examination: Blood, skin and eye tests, etc.

2-2. Education and Training (At the Number of Hours Specified by the Facility)• The number of hours differs depending on the facility, but please take the following training
courses.
• Take the training courses before entering the controlled area and for each period up to theend of the fiscal term in which the previous training was given after entering it.
Effects of radiation on human body (30 minutes or more)
Safe handling of radioisotopes, etc. or radiation generating apparatuses (1 hour or more)
Acts and regulations on the prevention of radiation hazards
and a radiation hazards prevention program (30 minutes or more)

3. Request an Order• Request the order for radioactive reagents to a Radiation Safety Manager.
• If purchasing from the Japan Radioisotope Association : Radiation Safety Manager places anorder with the Radiopharmaceuticals and Labeled Compounds Section of the Japan
Radioisotope Association.
Please refer to J-RAM for details.

• Check in advance with the Radiation Safety Manager so that your personal dosimeter canbe prepared on the day.
* You can also obtain radioactive reagents by transferring between facilities.
However, please check with the facility’s Radiation Safety Manager about the necessary procedures.

103) Method of Entering a Controlled Area

1. Completion of the Entry Record Book
• Fill in your name, purpose, entry location and time.
• There are also some facilities that manage this with key cards.

2. Advance Preparations• Put on your experiment clothes and wear a personal
dosimeter to monitor the amount of radiation you areexposed to. (As a rule, men should attach it to their chest
while women should attach it to their abdomen.)(3) Entry into the Controlled Area
• Take off your shoes and put on slippers for the controlled area.

Personal Dosimeter
Attachment Position(4) Entry into the Use Room
• Properly put on protective equipment (e.g., gloves and masks) before handling radioactive
reagents.

Take care because yourgloves are liable tobecoming contaminated♪

114) Receipt and Storage of Radioactive Reagents

1. Delivery and Receipt• In most cases, radioactive reagents are carried to a designatedlocation (e.g., the Radioisotope control room) by the operator. The
Radiation Safety Manager will receive the radioactive reagents.
• Manage the schedule to avoid it not being possible to receive or checkthe radioactive reagents for a long time after they have been carried.

2. Product Check• Remove the security bottle (outer container) after putting on
your gloves in the designated location (e.g., in the draft
chamber) and visually check there are no liquid leakages.
• Check that the compounds described on the vial label andshipping voucher are actually what have been ordered.

Security bottle
Vial

3. Contamination Check and Cardboard Disposal• Check with a radiation survey meter that the removed
shielding container and cardboard are not contaminated.
• Discard the cardboard after removing the labels, marks
and other stickers that indicate it is a radioactive
substance.

4. Storage and Record of Radioactive Reagents• Store and record radioactive reagents according to
the procedures of the facility.
• Fill out the Radioisotope name, amount ofradioactivity, date and your name on the container.
• Store in the storage facility (e.g., storage room orstorage box) at the appropriate temperature (normal
temperature, refrigerated or frozen) until use.

Manage the radioactive
reagents withresponsibility♪

125-1) Handling of Radioactive Reagents

1. Preparation of the Necessary Materials••••

Prepare in advance the things you will take into the controlled area based on your experiment
plan.
Take in as few things as possible and minimize the equipment you put out on the laboratory
bench to reduce the risk of contamination.
Once contaminated, you cannot take out anything no matter how expensive (e.g., watches) if
they cannot be decontaminated.
The following gives examples of what you will take into the controlled area.
Micropipettes

Tips

Tubes

Garbage bags and otherconsumables

Non-Radioisotope reagents

Writing materials

2. Measures Against Contamination• Perform work in a tray (affixing filter paper or similar to it is recommended) to prevent the
spread of contamination.
• If using polyethylene filter paper, you can prevent Radioisotope permeation contamination byusing it so that the polyethylene is on the bottom and the filter paper is on the top.

3. Preparation of Shielding Materials• In general, use an acrylic plate for Radioisotopes that emit β rays and use lead blocks for thosethat emit γ rays.
• Take care because high energy β rays emit bremsstrahlung rays with the same properties as γ
rays.
Shield from high energy β rays
with acrylic plates and lead blocks
in that order!

Bremsstrahlung

Excess energy appears whenchanging course
Acrylic

13Lead5-2) Handling of Radioactive Reagents

4. Background (BG) Measurement Before the Start ofYour Experiment• Natural radiation exists as BG.
• This also serves as the index when
conducting a contamination test.
Therefore, please measure it before
conducting your experiment.
• Set the radiation survey meter to amode that produces sound.
If you also leave the power on during
your experiment, it will help to preventthe spread of contamination.

5. Mock Experiment (Cold Run)
• We recommend you conduct a mockexperiment in advance using coloredwater as the radioactive reagent to reduce
the experiment time and to understandwhere contamination is likely to occur.

Is the equipment in a safe and easy-touse position? Have you assembled
everything you need? Is there anything
unnecessary or wasteful

6. Final Confirmation of Protective Measures
and Carrying the Reagents• Check that you are properly wearing the protective equipment (e.g., masks and arm covers).
• Take the radioactive reagents out of the storage room.
• Are there things you need to carry and move around in the laboratory?
If so, put them in a tray to carry them around. This reduces the risk dropping them. At thesame time, it ensures a distance with the Radioisotopes to lessen the risk ofcontamination and exposure.
Also check that there is a negative pressure
inside the draft chamber.
This is very important to prevent
contaminated air from flowing into the room
Please check with the Radiation Safety Manger forthe method to check there is negative pressure.

145-3) Handling of Radioactive Reagents

7. Opening of the Radioactive Reagents• Remove the vial containing the radioactive reagent from the shielded container using tweezers in
the draft chamber.
• If handling a radioactive reagent in the form of a solution, please open it after centrifuging it andcollecting the solution at the bottom of the container.
• There is a risk of contamination by opening and closing a vial repeatedly, so avoid this.
• Please use a clean pipette tip or syringe when removing the solution from the vial.

Example of Opening a Vial
You can easily open the vial lid by using a security bottle.
1) Open the lid of the security bottle.

2) Place the octagonal wrench on the lid of
the vial so it engages with the cap.

3) Turn the octagonal wrenchcounterclockwise and remove the cap.

8. Method of Fractionating Radioactive Reagents• Place the vial in a centrifuge and collect the solution
at the bottom.
• Adjust the differential pressure while paying attention
to venting from the container and then carefully open
the lid.
• Fractionate the solution with a pipette.
• Take care when opening and closing the lid
because contamination is liable to occur.

15

We have published themethod of fractionating
radioactive reagents using
syringes, needles andcharcoal filters on the web
version. We will also upload
videos in turn♪

5-4) Handling of Radioactive Reagents

9. Handling of Various Materials to Prevent Contamination• Basically, handle radioactive reagents in the same way as normal reagents.
There is a risk of contamination in the same way as with normal reagents.
• In addition, there is a risk of unintentional exposure due to contamination.
• Please frequently check for contamination with a radiation survey meter while working.
• We give tips for handling micropipettes below. We introduce conical tubes, microtubes andsyringes in the web version.

Micropipette Usage to Prevent Contamination
1) Volume• Allow for a surplus suction volume or use a tip with filter.

2) Tips for Suction and Discharge• Slowly and smoothly press the push button in.

3) Tip• Do not insert the tip deeply into the solution.
• Do not leave in a state in which the tip with radioactive reagent attached isinserted.
• Remove the tip using a tip injector without directly removing by hand.

10. Cleaning Up• Wash washable glass equipment.
• Carefully wash so that water does not splash outside after wiping off the attached solution
with paper in order from the equipment with the least amount of contamination.
• Conduct a contamination test on the equipment and locations you used to check that there are no
problems compared with the BG after washing and then return the equipment to its original
location.
• Store the remaining radioactive reagent in the storage room or dispose of it.

Wipe off withpaper

Wipe off withpaper

Contamination
test

Wash

16

6) Contamination Measurement

1. Overview

There is a direct method of
measuring directly and an indirect
method of measuring what has been
wiped off with smear filter paper.

• Radiation survey meters cannot measure everything.
Therefore, select a measuring instrument andmeasurement method appropriate for eachmeasurement target.

2. Direct Method• Use a radiation survey meter to check the strength of the radiation in the space and the surface
contamination of objects.
Types of Survey
Meter

Measurement Targets

Overview(For measuring surface
contamination)β, X and γ rays

Use to detect normal β rays with ageneral radiation survey meter.

Scintillation

X and γ rays: NaI radiation survey
meterΑ rays: ZnS radiation survey meter
125I: 125I radiation survey meter

A device that measures the air dose
per unit time.
Other than ZnS: basically used todetect γ rays

GM tube(For measuring the doserate)

3. Indirect Method (Smear Method)

3H and14C cannot be measured withthe direct method. Wipe off with thesmear method and then measure witha liquid scintillation counter.

• Select the smear method if it is not possible to measure it as it is or if yourmeasurement target is α rays and β rays (low energy β rays).
• Use special filter paper to wipe off rough surfaces.

4. Contamination Assessment• You can determine that there is no contamination if there is no significant difference with
the BG count rate.
• The assessment method differs depending on the radiation survey meter. Accordingly, pleasecheck that assessment method.

177) What to Do If There Is Contamination

Flow of What to Do If There Is Contamination• No matter how much care you take of contamination, it will still actually occur.
• If you confirm there is contamination, please seek the cooperation of those around you without
tackling it alone to prevent the spread of contamination.
• It is important to not be afraid, to not panic and to not take it lightly when dealing withcontamination.

(1) Clarification of the Contaminated Locations• Use a radiation survey meter toclarify the contaminated locations.

Once you know thecontaminated locations,
enclose them with tape tomake it easier to work♪

(2) Contact• Contact the people and places specified by the facility (e.g., Radiation Safety Manager andcontrol room).

(3) Removal Work• Perform removal work from the locations with the least amount of contamination so thatthe contamination does not spread according to the instructions of the Radiation Safety
Manager.
• Please repeatedly decontaminate until the contamination drops to the BG level.
Soak solution in paper
and then dispose of
the paper

Wipe away from theperiphery to the center

Measure again andassess(4) Report• Once you have finished removing the contamination, report the date, your name, results andother details to the Radiation Safety Manager.
• Contamination is inevitable. What is very important is dealing with it afterward.
• Contamination may remain even with a small amount of contamination you dealt with by
yourself.
Therefore, please make sure to report incidents to the Radiation Safety Manager.
• We have uploaded the detailed method of dealing with contamination in the web version.
Please check it out.

188) Disposal of Radioactive ReagentsContaminated Waste• Radioisotope waste produced in experiments using radioactive reagents has a different method of
disposal to regular waste.
• Please make sure to check the sorting and disposal methods with the Radiation Safety Manager.
• Please treat samples that have finished undergoing a series of operations as contaminated waste.
• Although it differs by facility, basically, garbage cans are established for each category below.

Category

Targets
Papers, cloths and wood chips

Combustibles

Flame retardantmaterials

Plastic tubes and poly-vials (please remove the remaining liquid),
poly-sheets, Styrofoam and other plastic products, rubber gloves
and other rubber products

Incombustibles

Glass vials (please remove the remaining liquid), glass instruments andother glass products, vinyl chloride products, hypodermic needles,
aluminum foil and other metal products, silicon products and Teflon products

Non-compressed
incombustibles

Metal lumps, steel frames, concrete pieces, cast metals,
machines and equipment, large quantities of beta plates, small
quantities of TLC plates, and large quantities of activated charcoal

Inorganic liquids

Organic liquids

Animals

Inorganic liquids, (e.g., waste washing liquids and cell culture liquids)(please do not mix with organic liquids)

Waste scintillator liquid (please do not mix with other organic liquids)

Dried animals, and bedding, floor coverings and sawdust towhich feces and urine are stuck

Non-contaminated Waste

Please treat samples that you havefinished experimenting on as
contaminated waste!

• Dispose of non-contaminated waste with the same disposal
method as in regular experiments.

199) Method of Exiting a Controlled Area

1. Enter the Contamination Test Roomand Wash Your Hands• Wash your hands before the contamination test.
Please thoroughly wash even up to your wrists.

2. Test Your Body and Articles for Contamination• Test your hands, feet and experiment clothes forcontamination with a hand and foot cross monitor.
• In addition, check the contamination of articles you willtake out of the controlled area with a radiation survey
meter.
• You cannot exit the controlled area without
undergoing a contamination test.
• Moreover, you cannot take out articles from thecontrolled area unless they fall below the take-out
standards.
• If there is contamination, contact the facility’s Radiation
Safety Manager and make sure to decontaminate
before exiting the controlled area.

Please test yourexperiment clothes forcontamination witha horizonal detector.

It is not possible to measure contaminationfrom 3H and 14C with this method.

3. Return Your Experiment Clothes and Personal
Dosimeter• Leave your experiment clothes in the controlled area and store your personal dosimeteroutside the controlled area after checking the reading.
• Leaving your personal dosimeter affixed to your experiment clothing or leaving it in the nondesignated position may lead to incorrect measurement results.

4. Complete the Entry Record Book
• Fill in the usage records, disposal records and time.
• There are some facilities that automatically record
information with IC cards.

5. Exit the Controlled Area

We introduce this in moredetail in the web version.
Please take a look!

(only Japanese)

20-

MEMO --

MEMO -

Hiroyuki Iizuka:
Shinsuke Katoh:
Masayuki Hara:
Keiichi Matsunami:(THE UNIVERSITY OF TOKYO, School of Engineering Environment, Health and Safety Office)(YOKOHAMA UNIVERSITY OF PHARMACY, Department of Health Pharmacy)

Cooperation:
Responsibility for publication:

Revvity Co., Ltd.
Radiopharmaceuticals and Labeled Compounds Section,
Radiopharmaceuticals Division, Japan Isotope Association(TOKYO MEDICAL AND DENTAL UNIVERSITY (TMDU), Institute of Research, Research Core)(JUNTENDO UNIVERSITY, Graduate School of Medicine)

2-28-45 Honkomagome, Bunkyo-ku, Tokyo, 113-8941
Mail: shiyaku@jrias.or.jp
Published in October 2019