Image scanner for electrophoretic gels

GELSCAN-3

Fluorescence imaging system for protein andDNA electrophoretic gels.
The uniformity of light realized
by the scanner makes it perfectfor quantitative measurements.
Fluorescent reagents: SYPRO ruby, ﬂamingo, SYBR gold, SYBR green, FITC, EtBr

GELSCAN-3 Features● No darkroom required. The same unit can be used forboth f luorescent and visible gels.

● Wet gel can be placed directly on the scanner.
● No focusing or exposure adjustment is required.
Uniformity of light and repeatability make thescanner ideal for use in quantitation.

● Values directly proportional to f luorescence intensity
can be obtained in 16 bits (65536 gradations.)● Max scan size is 310 × 437 mm. Several large gels canbe scanned at the same time.

Reagents supported by each model
For ﬂuorescent gels201801A1

For ﬂuorescent and visible gels201801A2

For visible gels201801A3

Model

201801A1

201801A2

201801A3

Drip-proof

✓

✓

✓

SYPRO Ruby✓✓

Flamingo✓✓

EtBr✓

✓

SYBR Green✓✓FITC✓

✓

CBB✓

✓

Silver staining✓✓※If you would like to try out a scanner before buying one, please avail of ourrental service.

1．Epi-illumination methodphotographic unit

Specifications

lightingequipment

Light source

The wet gel is scanned from above.
Scattered light due to surface
irregularities in the gel becomesbackground noise.

White LED array /Blue LED array (λ_peak = 468 nm)

gel

Sensor

CCD line sensors

Scan size

For ﬂuorescent gels /For ﬂuorescent and visible gels
309 × 424 mm (309 × 406 mm in visualization)
For visible gels

8 bit JPEG / Digital camera image

2． GELSCAN method

310 × 437 mm (309 × 420 mm in visualization)
Optical resolution

2400 ppi (11 μm)

Bit depth

RGB each 16 bit IN /16 bit OUT

Interface

Hi-Speed USB

Detection sensitivity

Protein: 1 [ng / band]
DNA: 1 [pmol / band］

Scanning time

75 seconds(8 cm mini gel electrophoresis, at 300 ppi)

Drip-proof

Gel adhered to platen glass isscanned from below.
The result is a smooth image
without scattering.
16 bit TIFF / GELSCAN image

Wet gel can be placed directly on the platen glass.

Scanner dimensions

W656 × D458 × H190 mm

Weight20 kg

Power consumption

65 WPower source

AC 100–240 V , 50/60 Hz

SoftwareiMeasureScan Std

Application examples● Two-dimensional electrophoretic images of proteins● Improvement of plant varieties

Fluorescent reagents: SYPRO Ruby, Flamingo

ﬂuorescent reagent: SYBR gold

Comparison between GELSCAN and molecular imager FX Pro

Documents provided by the Department of Biologicalfrom BIO-RAD.

Resources Management,

Exhibit: "JHUPO2008 P-39, An evaluation of the performance

The University of Shiga Prefectureof the latest version of the ﬂat-bed ﬂourescence scanner"

DNA polyacrylamide gel: electrophoretic images

Documents provided by Towa Environment Science Co., Ltd.

● Gel size : Two 25 cm x 12 cm sheets can be scanned at the same time.

● Conﬁrmation of ssDNA● A comparison between 2D electrophoretic images and images produced using
ImageMaster (GE)Bio-Rad Molecular Imager FX Pro (Left) / GELSCAN (Right)

area 1Conﬁrmation of ssDNA
ﬂuorescent reagent: FITC
Documents provided by Institute of Industrial Science,
The University of Tokyo

area 2

Q&A
Can the excitation wavelength offluorescence be changed?
What is the difference between the model(201801A3) for use with visible gels andother image scanners available on the market?

Does the variation in luminescence intensity
between the 108 LED chips affect thefluorescent image of the gel?

Excitation / fluorescence wavelengths can becustomized. Please ask for further information
at the time of purchase.

It is drip-proof.
Wet gel can be placed directly on the platen glass.

Images are not affected by diﬀerences in theintensity of flourescence.
The images are corrected automatically because"shading correction" is performed for each scan.
No.

Does the degradation in the light intensity
after the LEDs have been on for an extended
period of time degrade the value of theresulting image?

Developed and manufactured byiMeasure Inc.

2-3-33 Kaichi, Matsumoto, Nagano 390-0876 Japan
Tel: +81-(0)263-50-8651 Fax: +81-(0)263-50-8652

www.imeasure.co.jp"Shading Correction" is performed for each scan.
Moreover, the "exposure time" of the sensor is
automatically adjusted according to the lightintensity of the light source. This maintains theS/N ratio of the resulting image.
Sales agent