MyGoPERFORMANCE MADE EASY

USER GUIDEWELCOME
Thank you for choosing the MyGo Pro.
The MyGo Pro provides unmatched performancein a convenient format. Novel Full Spectrum Optics
deliver 120 optical channels of fluorescence datafrom every tube for exceptional multiplex PCR.
High-performance Peltier elements, combined withsolid silver blocks, provide both speed and provenworld-leading thermal uniformity.
Performance made easy...

2

CONTENT
This user guide will teach you what you need to know
to start running your MyGo Pro. It describes everythingfrom connecting your instrument to the network todata analysis for your qPCR experiments.
Contents include...

SETUP

A N A LYS I S

Learn how to install your
MyGo Pro - from setting it
up on your workbench, toconfiguring the instrument
and installing the software.

Learn how to easily
analyse your results.

E X P O RT
Learn how to export your datafor use with other software.

TIPS

MAINTENANCE

Learn some useful tips
for using your instrument.

Learn how to look after
your MyGo Pro.

EXPERIMENTTROUBLESHOOTING

Learn the basics of setting
up an experiment on your
MyGo Pro.

If you ever have a problem
with your instrument, learn
how to troubleshoot it here.

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SETUPI N S TA L L I N G YO U R M YG O P R O
You should have the following items in the MyGo Pro flight case:

1. MYGO PRO QPCR INSTRUMENT
2 . M YG O P R O U S E R G U I D E
3 . POWER SUPPLY UNIT AND MAINS CABLE
4. ETHERNET CABLE
5. USB DRIVE
Please keep the flight case and outer box the MyGo Pro camein, in case you need to protect your MyGo Pro during storage
or shipment in future.

Lid

USB Port

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LID
The lid ensures that tubes are seated correctly in the wells,
provides optical isolation, includes a heated lid to reducecondensation, and prevents dust falling into empty wells.

U S B P O RT
Insert the USB drive here to run an experiment from theUSB drive.

D I S P L AY L E D s
The MyGo Pro uses display LEDs to help you understand what
the instrument is doing. Here is a summary of these display LEDs.

Blue

Initialising

Green

Idle

Red

Lid open - Please close the lid

Yellow

Tubes loaded

Blue

Running, low temperature

Pink

Running, medium temperature

Red

Running, high temperature

Cyan

Experiment complete

Red-Flashing

Fault detected5

M YG O P R O CO N N EC T I O N S
The MyGo Pro has three connections. One in thefront and two in back as shown below:

Ethernet PortPower

E T H E R N E T P O RT

POWER
This is to connect your MyGo
Pro to the provided power
supply unit.

This is for connecting your
MyGo Pro to your LAN or
computer.

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POWERING ON YOUR MYGO PRO
Place the MyGo Pro on your lab bench, and thenconnect AC power to the power supply unit. Your
instrument will now turn on after a few seconds. The
display LEDs will light up blue, and then turn green if
a lid is closed or red if not. ​Your MyGo Pro is ready torun. The MyGo Pro uses a 3-pin IEC mains connector.
If you are not using an earthed supply then you must
provide an additional earth connection.

CO N N EC T I N G YO U R M YG O P RO
TO A N E T W O R K , P C O R L A P TO P
Connect one end of the Ethernet cable to the
instrument and the other end to a LAN port, or
directly to your computer. ​Once your MyGo Pro
is connected to a LAN, you can connect to yourinstrument via Wi-Fi if your network supports it.
A summary of these connections is shown on thefollowing page...

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8

MYGO PRO HEATED LID
The MyGo Pro heated lid will get hot. Please do nottouch it. The heated lid will be preheated to 105ºC
if user activity is detected. This enables your run tostart as soon as possible. After 5 minutes of inactivity
the heated lid will be turned off to conserve energy.

Heated Lid
Do not touch9I N S TA L L I N G YO U R
M YG O P RO S O F T WA R EYour MyGo USB drive contains software for Windows,
Mac OS X, and Linux operating systems. Please open
the software file matching your chosen operating
system. The latest version of the software can also be
downloaded from our website mygopcr.com.

WINDOWS

M AC O S X

Double-click​ ​on the
Windows installer and follow
the on-screen instructions
to install your MyGo Pro
software on Windows.

Double-click​on thedisk image and follow
the on-screen instructions
to install your MyGo Pro
software on your Mac PC.

10

CONFIGURING YOUR
MYGO PROWith your MyGo Pro software open, and your
MyGo Pro connected to the network, please connect
to your MyGo Pro Instrument.

Please select Configuration

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Please select MyGo Pro

To add your new MyGo Pro
select the Add b​utton. You
will now be presented witha list of available instruments.

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Double-click​on the
instrument you wish toconnect ​to, or​ pr​ess ​Select ​with the instrument selected.
The instrument will now
be added to the list of ​Registered Instruments
for use in the software.

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TIPS
Here are some great tips to keep in mind whilst running your
MyGo instrument.

1. LID GETS WARM
2 . D O N OT L E AV E T H E L I D O P E N
3 . SPIN YOUR TUBES
4 . R E M OV E A L L B U B B L E S
5 . K E E P YO U R L A B C L E A N
6 . K E E P YO U R I N S T R U M E N T C L E A N
7. PC SETTINGSL I D G E T S WA R M
The MyGo Pro lid can get warm during operation, this iscompletely normal.

D O N OT L E AV E T H E L I D O P E N
If the lid is open dust may fall into the wells and affect theperformance of your instrument.

S P I N YO U R T U B E SThis should ensure that all contents are at the bottom of thetubes, and there are no bubbles present. Reaction mixtures
which are viscous, or contain high levels of detergents, will
require stronger centrifugation to remove bubbles. Pleaseuse sufficient g-force to ensure that no bubbles are present.
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R E M OV E A L L B U B B L E S
Bubbles can cause optical artefacts as shown in the graphbelow. Ensure that no bubbles are present in reaction volumes.

Intensity (RFU)

ArtefactCycle

K E E P YO U R L A B C L E A N
Please keep your work space clean including all lab equipment
like surfaces, pipettes, and tube racks. This will keep the
instrument clean and help maintain good results.

K E E P YO U R I N S T R U M E N T C L E A N
We recommend a routine cleaning of your instrument. To do so
follow the cleaning guide in the Maintenance section.

PC SETTINGS
Please disable all power saving settings e.g. sleep and hibernate.

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EXPERIMENTThis section will teach you everything you need to know
to get started with MyGo Pro experiments. You will learn
how to create, save, open, and close experiments. You will​
also learn how to set up an experiment, including thermal
profile, sample information, and optical settings. Finally
we will show you how to run your new experiment fromthe software, and from the USB​drive ​provided.

CREATE A NEW EXPERIMENT
Below is the MyGo Pro home screen.

Click New to create
a new experiment.
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E X P E R I M E N T S U M M A RY
Once you have created a new experiment theE​xperiment Summary w​ill be displayed as shown below:

By default the experimentname will be “N​ew
Experiment” ​with a date
and time stamp, which
can be edited.
The summary will give
you information about the
instrument you are running,
the settings chosen, and any
useful notes you choose to
add about your experiment.

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OPEN AN EXPERIMENT
By selecting O​pen y​ou can open an experiment asshown below:

FILES OF TYPE
The F
​ iles of Type drop downmenu gives you the option to
open an experiment normally,or as a template that can beused for a new run.

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EXPERIMENT FILE
An experiment file will containall the data and analyses, but
can not be re-run.
To open as an Experiment
File s​elect the first option
shown on the opposite page.

EXPERIMENT FILEA S T E M P L AT E
Opening an experiment file
as a template allows you to
use the same profile, samples,
settings, and analyses to
re-run a previous experiment,
generating new data. When an
experiment file is opened asa template, it has the same:
1. Temperature profile.
2. Optical settings.
3. Samples and targets.
4. Analyses, including settings.
If you wish to open anE​xperiment File as Templates​elect the second option.

USB RUNTo open a USB run navigate to the USB location using the Opendialog window shown to the left. Select Experiment File in theFiles of Type drop down menu, and then double click on the
USB run.
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SAVING AN EXPERIMENT
Once an experiment has completed it can either be
automatically saved to a pre-set location on your computeror manually saved by you. The experiment must be saved
before the software is closed to ensure that data is not lost.

AU TO M AT I C
SAV I N G

To automatically saveyour data when the run
has finished, firstly click
Browse and choose thelocation to save your run to.

When starting a run you will
be prompted with the window
displayed below.

Make sure the first checkbox is selected and yourexperiments will be automatically saved. To preventthe software from showing this dialog again, select thesecond checkbox. You can change these settings laterby clicking Configuration and selecting General Config.

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M A N UA L SAV I N G
Once your run has finished you can save your experimentmanually by clicking Save or Save As. The first time anexperiment is saved, you will be prompted to select a locationand filename to use. After this you can click Save to use thesame location, overwriting the previous version, or Save As tochoose a new location.

C LO S I N G A N E X P E R I M E N T
To close an experiment select C​lose. ​If the experiment has notbeen saved the following dialog box will appear:

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SETTING UP A PROFILE
In this section we will cover Hold Times, Programs andsetting up a temperature profile.
HOLD TIMES
During thermal cycling protocols hold times should be chosen
to allow for the following: thermal equilibration of reaction
volumes; completion of biochemical reactions, for example fulllength strand extension; and optical data acquisition.
Typically, optical data is acquired at the same temperaturethat strand extension occurs. By default this time is set to 30s,
However, this time can be reduced to a minimum of 17s, to savetime, or increased to more than 30s in order to increase PCR
efficiency and optical sensitivity.
For other hold times during cycling protocols, by default times
are set to 10s. However, this time can be reduced to a minimumof 3s, to save time, or increased to more than 10s in order toincrease PCR efficiency.

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A thermal profile is required to perform an experiment.

Select the profile tab as shown below:

PROGRAMS
To add a program to your experiment clickA​dd i​n the P​rograms p​ane. Programs canbe deleted by selecting them in the list thenclicking D​elete. ​The program order can also
be altered by selecting the program to movethen clicking the U​p a​nd D​own arrows.

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T E M P E R AT U R E P R O F I L E
Once programs have been added to the experiment they willappear in the T​emperature Profile p​ane in the order they arepresent in the P​rograms ​pane as shown below:

PROGRAMSETTINGS
To alter the settings ofa particular program you
should first select it, thenrefer to the right hand ​pane.
In​the example above the ​Melting program is selected
and can be edited in theright hand pane.
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SAMPLES
You will now learn how to set up samples and targets
which can be defined as follows:
SA M P L E S

TA R G E T S

A description of the specimen
being analysed e.g. “Mouse #5”

A molecular target e.g. “x174”,detected with a specified
fluorescent reporter e.g. “FAM”

Samples or targets do not need to be defined
before starting a run. They can be added toan experiment, whilst it is running, or once it
has finished. With a new experiment created,
please select the ​Samples tab as shown below:

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A D D I N G SA M P L E S A N D TA R G E T S
Samples and targets can be added and removed fromthe experiment by selecting + and - in the ​Samples
and Targets panes.
The order of samples and targets can be alteredby clicking the ​Up and D​ own arrows in the ​Samples
and Targets panes.

A S S I G N I N G SA M P L E S TO W E L L S
Assign wells by selecting them and clicking ​Set.
Samples can also be unassigned by selecting
wells you wish to un-assign and clicking ​Clear.

A S S I G N I N G TA R G E T S TO W E L L S
Targets can be assigned to wells as a Standard,
Unknown or Negative by clicking Std., Unk., or Neg.,
respectively. Assignments can be cleared by clicking
Clear with the targets and wells you wish to clear
selected. For more information about different types ofstandard controls please contact technical support.

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DY E CO M PAT I B I L I T Y
The MyGo Pro comes pre-calibrated for over 20 different
fluorescent labels, and the software enables users to use any dyewith an emission maximum between 510nm and 750nm. A listof the available dyes on the MyGo Pro can be viewed below. The
Auto Dye File Generation software analysis module enables you tocalibrate your MyGo Pro for additional fluorophores if required.

Factory Calibrated Dyes

		SYBR Green I, ResoLight,
FAM, VIC, HEX, Yellow 555,
Red 610, TexasRed, Cy5, CAL
540, CAL 560, CAL 590, CAL
610, CAL 635, JOE, Pulsar 650,
Quasar 570, Quasar 670, 		Quasar 705, ROX, TAMRA, TET

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SAV I N G A N D O P E N I N G A SA M P L E S E T U P
If you wish to save or open sample and target information setup
you can select ​Save As., or Open, respectively.

Sample and target setups can be saved in the following formats:

CSV

Editable .csv files.

PSD

A proprietary locked file format.

RDML

The Real-time PCR Data Markup Language (RDML)
is a structured and universal data standard forexchanging quantitative PCR (qPCR) data.

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RUN SETTINGS
The MyGo Pro run settings tab is where you will
determine the optical settings, view your dye
calibrations and access the advanced settings.
OPTICS SETTINGS
The MyGo Pro will aquire optical data during thehold times. The longer the hold times are the better
your optical data will be. Different reagents canrequire different optical settings.
I N T E R C A L AT I N G DY E S

These dyes, for example SYBR Green I, ResoLight and EvaGreen,
are bright reagents and require a shorter integration time.
S EQ U E N C E - S P EC I F I C P RO B E S

These dyes, for example FAM, VIC and HEX, are commonlyused for TaqMan genotyping or multiplex experiments.
Hydrolysis probes are not as bright as Intercalating dyes
and therefore require a longer integration time.

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I N T EG R AT I O N T I M E

Integration Time is the exposure time, in seconds, of the​optical​
sensor inside the MyGo instrument during thermal cycling.
Brighter reagents require shorter integration times than dimmer
reagents.

DY E C A L I B R AT I O N S
Here is where you can review the current dyes present
in your experiment. You have the following options
when working with dyes in your MyGo Pro software:
OPEN

RENAME

Open dye files from a file tobe added to this experiment.

Rename your dye files toyour liking.

SAV E

U P DAT E F RO M D E V I C E

Save a dye file from yourexperiment to a file on yourcomputer. These can then be
used on multiple computers
and MyGo Pro instruments.

Upload dye files from your
MyGo Pro to your new
experiment.

R E M OV E

Remove dye files from thisexperiment. Factory calibrated
dye files can not be removed.

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A DVA N C E D S E T T I N G S
Here, if necessary, you can tailor optical settings to
the characteristics of your assay.
You can adjust Amplification Integration Time toincrease, or decrease the sensitivity of the MyGo Pro
fluorescence detection system. Longer integration
times will provide improved signal to noise with dim
fluorescent reporters. Short integration times will
reduce the risk of the fluorescence detection systembeing saturated by very bright fluorescent reporters.
For your convenience, at the end of every run, your
MyGo Pro will record a Recommended Amplification
Integration Time in the Run Log. Runs where every
well contains a strong positive sample will enable
your MyGo Pro to estimate the most accurate
Recommended Integration Time for your assay.
O P T I M I S E M E LT ACQ U I S I T I O N S

By default your MyGo Pro will use automatically optimised
integration time settings during melting analysis. If you wantyour MyGo Pro to use the Amplification Integration Timeduring both amplification and melting analysis, deselect thistick box.

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STARTING A RUN FROMTHE SOFTWARE
To start a run from the MyGo Pro software selectS​tart Run. Y​ou will then be presented with the autosave options (unless you have chosen not to beprompted) and then be asked to choose an instrumentfrom the list of R​egistered Instruments. ​Select an
instrument and press S​elect t​o begin the run.

STARTING A RUN FROM
A USB DRIVE
To start a run using a USB drive select ​Start Run.
Then, when you are asked to choose an instrument,
select ​Start run from USB. You will then be prompted
to find the location of your USB drive. Once selectedpress ​Save and safely remove your USB drive as normal.
The USB drive can now be placed into the instrument
and your experiment will start automatically.

LABELLING YOUR STRIPS
You can write on the lids of your stip caps. Please do notwrite on the sides of your strip tubes. Ink can transferfrom the sides of tubes to the walls of the wells, and thiscan affect the performance of your instrument.
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LOADING STRIPS INTO
YOUR MYGO PRO
In order to ensure that the heated lid is balanced,
please make sure that the mount contains at leasta strip in rows A and D (as shown in blue below)
or single tubes in wells A1, A8, D1 and D8. These
positions can be filled with tubes containing reagents,
or empty tubes.

1

2

3

4

5

A
B

C
D

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6

7

8

ANALYSISThis section will teach you about different typesof analysis available. These can be grouped into thefollowing groups. For information about more analysis
options please contact technical support.
Q UA N T I F I C AT I O NDetermine accurate quantities
of template or relative expression
levels of genes using Absolute
Quantification ​or R​elative
Quantification. ​G E N OT Y P I N G
Use TaqMan probes or High
Resolution Melting to perform
SNP genotyping using A​utomatic
Endpoint Genotyping ​orA​utomatic High Resolution Melt. ​M E LT I N GAutomatically determine themelting temperature of youramplicons using A​utomatic
Tm Calling.

RUN FUSION
Combine data from multiple
runs on your MyGo Pro for
analysis together.
34

SELECT ANALYSIS TYPE
Once your experiment has completed you can add ananalysis type by selecting the “+” button in the bottom
left of the software window.
Analysis types are separated into ​Automatic and M​ anual sections. ​Automatic analysis types use automated data processing toprovide accurate results with minimal user intervention. ​Manual
analysis types enable users to apply traditional analytical methods.

Ticks and crosses indicate
which analyses can be applied
to the data in your experiment.

35

AU TO M AT I C Q UA N T I F I C AT I O N
P O S I T I V E / N EG AT I V E C A L L I N G
The first step in amplification analysis is to determine whichtargets have produced a positive amplification curve, and which
amplifications have produced a negative amplification curve.
For every amplification curve the software calculates
three metrics, these metrics are used to determine which
amplifications are positive. These metrics are:
Q UA L I T Y

Quality is a measure of how clean an amplification appears.
This value will tend to be higher for amplifications with a clear
exponential phase and steady baseline, and lower for noisier orflatter curves.

High
Low

36

INTENSITY

Intensity is a measure of the size of the exponential phase of theamplification. Note that this may be smaller for amplifications
with a negative baseline drift.

High
Low

PERFORMANCE

Performance is a measure related to the observed efficiency of
amplification at later cycles. This value will tend to be lower for
inhibited or out-competed reactions, and higher for efficient reactions.

High

Low

37

SETTINGS
The software uses a combination of Quality (Q), Performance(P), and Intensity (I) thresholds to determine if an amplificationis positive (green zone) or negative (red zone). All values (Q, P
and I) must be above the relevant threshold for an amplification
to be called positive. The Settings tab enables you to review
metrics for amplifications and set appropriate thresholds.

The dots represent individual amplification curves in yourexperiment. All dots that are within the top right of thegraph are called positive and will be subject to normalization
determined in Advanced Settings.
To switch between “Intensity vs Quality” and “Performancevs Quality” select the Graph Axes drop down menu. All 3
thresholds are used for calling even if they are not currently
displayed on the graph.

38

A RT E FAC T F I LT E R I N GBiochemical and physical factors can cause fluorescence levels
to change during a run. These are often observed during early
cycles. Filtering out these artefacts can improve the accuracyof amplification analysis. Two settings enable you to control theprocess of filtering out such artefacts.
E XC L . C YC . M I N .

The software will exclude at least this number of cycles fromdata before performing analysis.
AU TO E XC L . C YC .

By default the software will automatically determine which
cycles at the beginning of a run exhibit artefacts, and exclude
this number of cycles from data before performing analysis.
This feature can be disabled by deselecting this option.

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N O R M A L I SAT I O N O P T I O N S
Here you can choose the way your data is normalised before
display in the amplification graph. By default Backgroundnormalisation is applied to assist data visualisation whentroubleshooting challenging assays. Full normalisation enables
the discrimination of subtle differences in amplification kinetics.
You can choose between the following modes:
Normalisation Mode
None

Background

Full

Background Correction
Normalisation

BAC KG R O U N D CO R R EC T I O N

Background fluorescence, including drift, is estimated andremoved. There are many potential sources of background
fluorescence, e.g. emissions from unquenched probes.

N O R M A L I SAT I O N

Positive amplifications are rescaled to account for intensity
variation between wells. Uncorrected intensity can be affected bya number of factors, e.g. the optical properties of disposables.
Normalised amplifications are rescaled to a nominal value of 1
relative fluorescent unit, with exact endpoint values a functionof curve shape.

40

R E S U LT S A S TA B L E
The results of amplification analysis are presented in the
Results as Table view.

Q UA N T I F I E R S
If you have assigned standards with different known quantifiers
to your experiment, for example a dilution series, you will be able
to see your reaction efficiency by selecting Quantifiers. Here you
will be shown the relationship between Cq and input template
quantity, plus a variety of statistics derived from the standards.

R E L AT I V E Q UA N T I F I C AT I O N
If you are performing relative quantification analysis then selectR​elative Quantification a​t the top of the A​uto Quant. pane.

41

M U LT I P L E X I N G
The MyGo Pro supports multiplexing with a broad variety of dyes.
F O R B E S T R E S U LT S :• Ensure that there is no competition between the
different PCR in the multiplex reaction.
• Ensure that probes have low background fluorescence,for example by using dual-quenched probes.
• Ensure that probe signal strength is balanced, by using
an appropriate concentration of primers and probes.
• Make sure to select the correct dye for each Target.
• Generate assay specific dye files if necessary.

TIPS
Spectral properties of fluorophores can vary as a result ofmany factors, including stereochemistry, sequence context
and buffer chemistry. If you observe spectral crosstalk,generate assay specific dye files to compensate forthese effects.

42

DY E F I L E G E N E R AT I O N
DY E E X P E R I M E N T
Dye file generation allows you to calibrate your MyGo Pro
for new dyes that are not included in the factory-calibrated
list. This calibration is based on a PCR amplification using therelevant dye or labelled probe.

DY E C A L I B R AT I O N R U N
You will need to perform a real time PCR run in order togenerate a dye file. Every well of your MyGo Pro should
contain the same reagents. These reagents should produce asingle PCR product, and a strong signal for the dye that you
are calibrating. Create a sample named after the dye that you
are calibrating, and assign that sample to all wells.

43

AU TO DY E C A L I B R AT I O N
Select the Auto Dye Calibration option in the Select Analysis
Type window. The Auto Dye Calibration analysis module will
analyse changes in fluorescence during the dye calibration run
to create a new dye file.

DY E DATA

Select this tab to view the wells that will be used in thecalibration.
A M P L I F I C AT I O N

Select this tab to view the cycles that will be used in thecalibration.

44

E X P O RT C A L I B R AT I O N
Select the Dye Data tab and you can choose to export thecalibration to a file, or, use it in your current experiment.

Select the Use Wells drop
down menu and you will see
the list of samples present in
the experiment. Select the
sample you wish to create adye from.

U S E C A L I B R AT I O N I N C U R R E N T E X P E R I M E N T

By selecting this option your new dye file will appear in thetarget list in this experiment. This option will not save the dyefile to your computer.
E X P O RT C A L I B R AT I O N TO F I L E

Alternatively you can save this new dye file to your Mac or PC.

45

G E N OT Y P I N G
AU TO M AT I C E N D P O I N T G E N OT Y P I N G
TaqMan genotyping experiments can be analysed automaticallyusing A​utomatic Endpoint Genotyping. ​Genotypes of samples
are determined by the ratio of endpoint fluorescence between
two TaqMan​​probes. Thresholds for the fluorescence ratios are
generated automatically as shown below:

AUTOMATIC HIGH RESOLUTION MELTING
A​utomatic HRM c​an be performed by using theA​utomatic HRM m​odule as shown on the following page:46

D I F F E R E N C E , N O R M A L I Z E D A N D M E LT

Once opened the analysis module will automaticallydetermine optimised parameters and generate a set ofD​ifference, Normalized a​nd M​elt g​raphs.
C LU S T E R I N G

The difference curves will then be converted into aclustering graph showing clusters of different genotypes.

G E N OT Y P E S

The software will automatically determine I​nferredgenotypes b​ased on the clustering graph. You can define yourcontrol G​enotypes u​sing the G​enotypes p​ane.

47

M E LT I N G
AU TO M AT I C T M C A L L I N G
Add an Automatic Tm Calling analyses to identify andcharacterise melting peaks. You can see the results below.

Tm values will
be presented
in the table.

48

AU TO S E T T I N G S
The user has the option
to change the target if
the experiment contains
more than one target. The
signal-to-noise ratio setting
determines how big a peakshould be before it is called.
Increasing this value will
increase specificity ofpeak detection. Decreasing
this value will increase thesensitivity of peak detection.

P E A K S A N D M E LT S
The P​eaks pane will show
you the melt peaks withmelting point indicators.
The M​elts pane will show
you the melting curvesof the same reactions.

49

RUN FUSIONRun Fusion enables you to combine data from multiple
runs from your MyGo Pro together for analysis. To fusemultiple experiments together hold down ​cmd on a
Mac or Ctrl on a PC and select the files you wish to
fuse from the open dialog window as shown below.

Select ​Open and then from thenext dialog select ​Fuse 2 files
into one. You can also open
files as separate experiments
using ​Open 2 Files.

50

If you are fusing experiments from multiple file locations you
must select the first experiment and then select the “+” from the
top left of the open file dialogue window as shown below.

Now hold cmd/Ctrl andselect the next experiment.
Select ​Open to fuse yourexperiments together.

51

EXPORT
When you have finished analysing your data in the
MyGo software, you can export the results in a variety
of ways, from raw data to user defined custom
reports. This section will take you through thesteps you need in order to do this.

TABLE EXPORTFrom panes showing
results in tabular format
you can export data using
the Export button.

52

To export data click the Export button, shownon the previous page. Data can be exported in thefollowing formats:
CSVThis is an editable data file that can be opened inmany spreadsheet applications.

PDF

This file format is suitable for ​archiving, printing​and presentations, but cannot be edited.

RDML EXPORTThe software is able to export Cq values andamplification curves from Auto Quantification andmelt curves from Automatic Tm Calling in RDML
format. To do this select Save as, as if you were saving
your experiment. Now from the Files of Typedrop down menu, select Real-Time PCR Data Markup
Language as shown below:

53GRAPH EXPORTFrom panes showing results as graphs, such as
amplification and melt curve data can be exported
by selecting the button from the below example.

The user can export information as bitmap images (.PNG),
Scalable Vector Graphics (.SVG), Character-Separated Variable(.CSV), or Portable Document Format (.PDF).

PNG

This is a bitmap image.

SVG

A Scalable Vector Graphic is an XML-vector based graphicthat can be used where a high resolution scalable graphis required, for example for papers or web design.

CSV

This contains information about the graph you
are exporting in spreadsheet format.

PDF

This contains the graph you are exporting in the
Portable Document Format. The graph is in vector
format for higher quality.
54

R E P O RT G E N E R AT I O N
Customisable reports can be generated from your experiment
by selecting ​Experiment Report under the ​Experiment tab.
This provides you with the ability to choose which parts of thedata to include​in a report. ​For example, items such as thermal
profiles, sample information and various analysis results, e.g. Tmvalues or quantities can be ​included, or excluded from reports.

Please select Experiment Report.

55

MAINTENANCE
This section will help you take care of your MyGo Pro. ​It covers ​the following​areas:
1. CLEANING

3 . ENVIRONMENTAL
CO N D I T I O N S

2. D I S P O SA B L E S

4 . D I SA S S E M B LY

56

CLEANING
DAY-TO - DAY
For day-to-day cleaning wipe the external surface ofyour instrument with a damp, soft, lint-free cloth. Then
dry your instrument with another soft, lint-free cloth.
Notes: Avoid abrasive cloths, towels, paper towels, andsimilar items that might cause damage. Before cleaning yourinstrument unplug all external power sources, devices, andcables. Do not get moisture into any openings.

CLEANING WELLSIt is important to keep the wells of your instrument
clean. Automated background subtraction processes
mean that low levels of fluorescence contaminationin the wells of your instrument will not affect system
performance. Dirty wells however, can result in
lower fluorescence readings, and can affect heattransfer from wells to tubes. Regular cleaning ofyour instrument’s wells will help prevent dirty wells
affecting the performance of your instrument. The
MyGo Cleaning Kit has been designed to enable you
to clean the wells of your instrument.
For details of the MyGo Cleaning Kit please visit:
http://www.mygopcr.com/cleaning-kit/
Notes: Do not use any other method or materials to clean thewells of your instrument. Doing so may damage yourinstrument.
57

D ECO N TA M I N AT I O N
If your instrument needs decontaminating please
follow the instructions contained in the MyGo
Decontamination Guide.
For details of the MyGo Decontamination Guide please visit:
http://www.mygopcr.com/decontamination/
Please note that for health and safety reasons you must print andcomplete a physical copy of the decontamination form, and include
this with any instrument or lid that is returned. The form must be sentwith the instrument, and we can not accept electronic versions of theform. Failure to comply with these guidelines will result in a charge fordecontamination.

DISPOSABLES
We recommend the use of MyGo disposables
for optimal results. If you wish to use third party
disposables please note the following:
A . Physical dimensions should be the same as the
MyGo disposables to ensure that they fit into yourinstrument, without damaging it.
B. Caps should seal tubes effectively in order to prevent
variability caused by evaporation, and to minimise
the risk of contamination with PCR products.

58

C . Disposables should have reproducible wall 		
thicknesses, which are thin enough to ensure rapid
temperature equilibration, but thick enough to 		
avoid breakages.
In many cases third party disposables will have inferior
thermal and optical characteristics, which will reduce thequality of results obtained from the system.

ENVIRONMENTAL CONDITIONSYO U R W O R K S PAC E
Your MyGo ​Pro should be placed on a surface that isflat, dry, and not subject to draughts. Do not installyour MyGo ​Pro instrument directly in the flow of air
from an air conditioner or fan.​Do not install your
MyGo Pro instrument in a dusty environment.
P R E V E N T I N G CO N TA M I N AT I O NPrevent contamination by wearing gloves, using clean
tube racks, and filter tips. Make sure tubes are sealed, PCR
product is disposed of, and leaks are cleaned immediately.

59

E N V I R O N M E N TA LO P E R AT I N G CO N D I T I O N S
Humidity

MAX: 80% at +32°C
MIN: 30% at +15 to +32°C

Temperature+15°C to +32°C

Pressure

0 to 2000 MAMSL
80 to 106Kpa

E N V I R O N M E N TA L S TO R I N G /T R A N S P O RT I N G / PACKING CONDITIONS
Humidity

Temperature

10% to 95%
No condensation-20°C to +60°C

Pressure

0 to 3000 MAMSL
70 to 106Kpa

D I SA S S E M B LY
Please note that your MyGo Pro contains no userserviceable components inside. Any disassembly of
your MyGo Pro instrument will void all warranties.

60

TROUBLESHOOTINGThis section will help you troubleshoot your MyGo Pro
if you think something is wrong.

FAQ
Here are some frequently asked questions,and the answers.
How do I create a template?

All experiments can be used as atemplate. Click “Open” and thenusing the “file type” selectionin the file dialog to select the“Template” option, then selectthe file to open as a template.

What is the minimum holdtime for thermal cycling?

For holds during thermal cycling
with no optical acquisitions theminimum hold time is 3s. For
holds during thermal cycling
with optical acquisitions theminimum hold time is 17s.

Can I leave my MyGo Pro
powered on overnight?

Yes. It is safe to leave your MyGo
Pro overnight, it will enter a low
power standby mode.

61

Where is the threshold for
determining Cq values in
Auto Quant?

Modern methods ofdetermining Cq values are notbased on simple thresholds.
Modern methods of Cq
determination are model
based. Auto Quant fits a modelof a PCR amplification to thefluorescence data observed.
This model fit then enables
the estimation of a numberof important parameters
including Cq values.

Why do some of myamplification curves drop
down before rising in
Auto Quant?

Correct background removalin Auto Quant relies oncorrect qualitative calling.
Check that appropriate
thresholds are set so that
positive amplifications arecalled positive.

Why is my Cy5 reaction
not working?

Cy5 is a relatively dim reporter,
you may need to increase
Integration Time to improvesignal to noise with Cy5
labelled hydrolysis probes.

62

ERROR MESSAGESYour MyGo Pro will let you know when somethingis wrong by displaying an error. Most errors arereported as messages in the Status Bar in the MyGo
software. Some errors are reported by the instrument
display LEDs.

Status Bar.

63

If you encounter an error, please make sure that the instrument
is running in a lab within the specified environmental conditions,tubes have been loaded correctly, the lid has been fitted
correctly, and all cables are attached correctly and securely.
If the error still occurs, you may need to contact technicalsupport with the following actions described below.

E R R O R M E S SAG E S D I S P L AY E D I N S TAT U S B A R
Message

ActionSaturation Warning.

Please use the log button
to view the detailed message- this will tell you which wells
saw a saturated reading.
To correct, please reducethe integration time setting,or reduce the concentrationof dye or probes.

Run Prepare Error(followed by one of thefollowing messages)

Please follows the instructionsin the error message andcontinue as normal.

a) No tubes loaded.
b) Instrument has had a
hardware error, and is in
failsafe mode.

64c) Instrument is not yet
ready to run.
d) Instrument has finisheda run and is waiting fortubes to be removed.
e) Open lid, remove tubes
and then add new ones.
FAILUREThis message will be followed
by a short code. Pleasecontact Technical Support.

Instrument connection
too slow.

May be reported at the startof a run. Please check thatyour network is properly
configured and is operating
at 100Mb/s or more.

Network error.

Please check that yournetwork is properly
configured and is operating
at 100Mb/s or more.
Always leave your laptop or
PC turned on throughout
the run, and do not use
any sleep, power-saveor hibernate function.
Do not close laptop lid.
Alternatively, perform therun using a USB drive.
65

E R R O R S R E P O RT E D BY
T H E M YG O P R O D I S P L AY L E D S
Some errors are reported by the instrument display
LEDs flashing red. If your instrument display LEDs areflashing red, please contact technical support.

ERROR LOG FILES
In the unlikely event that you encounter a problem
the software can produce log files. These files help todiagnose the problem.
S O F T WA R E E R R O R S
For software issues go to ​Configuration > ​About andselect ​Save Software Logs to File.
H A R D WA R E E R R O R S
For Hardware faults go to Configuration > ​MyGo Pro
and select ​Retrieve Instrument Report Files. Once
exported, please make sure you send the error file, theserial number, and the experiment file that contained theerror to Technical support. It is OK to send experimentsthat have not completed and/or were aborted.

66

NOTES

67

68

Permitted use case
The MyGo Mini instruments are to be use for Research Use only (RUO) and not to be used in an in vitro
diagnostic (IVD) setting.
All trademarks are the property of their respective owners.
Design and specifications are subject to change without notice.

69

MyGo
www.mygopcr.com

Version 004 (EN)