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MAST CARBA PAcE®

Pseudomonas, Acinetobacter, Enterobacterales
Rapid carbapenemase detection• Rapid results inless than 10 minutes• Simple test processin 5 easy steps• Cost effective

screening solution• High sensitivity and specificity

MAST CARBA PAcE®

An increase in antimicrobial resistance is one of the most urgent global challenges facing the field ofhealthcare. Healthcare associated infections (HAI) are one of the most common adverse effects in caredelivery and the occurrences of epidemics of HAI are a major public health problem (WHO 2017).
Carbapenem resistant Gram negative bacteria, including Enterobacterales (CRE), Pseudomonas aeruginosa
and Acinetobacter baumannii are an emerging cause of HAI and accordingly a global public healthcare
concern due to high levels of antimicrobial resistance and ability for widespread transmission.
It is important to recognise carriers quickly to prevent and control the spread in nosocomial and community
settings and also to aid antibiotic stewardship.

MAST® CARBA PAcE
Mast Group Ltd. remains at the forefront of the fight against such threats and as such has developed acolorimetric test which rapidly detects carbapenemase producing Pseudomonas spp., Acinetobacter spp.
and Enterobacterales using a novel chromogenic cephalosporin analogue for which there is a patentpending for its application.
The new MAST® CARBA PAcE is a simple cost effective screening solution providing fast results within
10 minutes, yet reliable in detecting carbapenemase production to support patient treatment. Additionally,no special equipment is required allowing it to be easily integrated into any laboratory workflows.
The basis of this colorimetric test is the hydrolysis of the chromogenic cephalosporin analogue, eliminating
the effects of ESBL and AmpC enzymes using suitable inhibitor compounds, providing specificcarbapenemase detection. Therefore only distinguishing epidemiologically significant carbapenem
resistance, avoiding unnecessary patient isolation.					Benefits of MAST® CARBA PAcE
Rapid time to result–			Once the test organism is added to the test 			solution results are available within 10 minutes

Cost effective– Conveniently contains 48 tests per kit, allowing quick			 identification of carbapenemase producers, assisting			 with prompt isolation of carriers to prevent cross			 transmission
Easily integrated into any laboratory –			Requiring only standard microbiological supplies 		making this adaptable to any laboratory workflows

Easy to Use–						

Simple test procedure, with 5 simple steps (see figure 1),colour change from yellow to orange/red indicates 		carbapenemase activity

Quality–						

Validated using a panel of organisms including VIM, IMP,NDM, OXA-48-like, OXA-23 and KPC high sensitivity andspecificity of 96% and 91% respectively

Supports effective IPC–			Rapid and reliable detection supports infection 			prevention and controlMAST CARBA PAcE®

Simple test Process
Reconstitute

Reconstitute the pellet bytipping the entire contentsof vial RB into vial PEL.

Dispense

Allow the pellet tofully dissolve at room
temperature for 1 minuteand mix contents by gently
vortexing for 10 seconds.

Dispense 250µl ofreconstituted solution into
the tubes provided.
One tube per test.

Use a pure, fresh culture of
the test organism.

Take an approximate
1 - 5µl loopful of organism.

Add to the tube containing
test solution.

Incubate

Read by eye

Inoculate-Incubate at 35±1°C for
10 minutes.

++++++

Record the colour of the test solution immediately or up
to 20 minutes after incubation.

Figure 1 - MAST® CARBA PAcE Process

Mix well by vortexing for
20 seconds.

MAST CARBA PAcE®

Ordering Information
Order Code

Product

Pack Size

No. Tests

PACE-ID

MAST CARBA PAcE
For the rapid detection of carbapenemase producing
Enterobacterales, Pseudomonas spp. & Acinetobacter spp.

4 × 12 tests

48

Pack Size

No. Tests®

Additional products from Masts AMR range
Order CodeProduct

D73C

MASTDISCS Combi Carba plus

5 × 50 discs

50

D72C

MASTDISCS Combi AmpC, ESBL & Carbapenemase
Detection Set

6 × 50 discs

50D74MAST®ICT Indirect Carbapenemase Test

2525

D71C

MASTDISCS ID CAT-ID - Carbapenemase Activity Test

5 × 50 discs

250

®
®

®

References
Guidelines for the prevention and control of carbapenem-resistant Enterobacteriaceae, Acinetobacter baumannii and Pseudomonas aeruginosain health care facilities.World Health Organisation 2017.
Acute trust toolkit for the early detection, management and control of carbapenemase-producing Enterobacteriaceae (Published: December 2013
PHE publications gateway number: 2013314)

Acknowledgement
HMRZ compound used in this product was developed by Dr. Hideaki Hanaki of Kitasato Institute, Japan.

ZT233 v2.0 CA 06/19

United Kingdom
Mast Group Ltd.
Mast House
Derby Road, Bootle
Merseyside L20 1EA

Germany
Mast Diagnostica GmbH
Feldstrasse 20
DE-23858 Reinfeld

FranceMast Diagnostic
12 Rue Jean-Jacques Mention
CS 91106
80011 Amiens CEDEX 1

Tel: + 44 (0) 151 933 7277
Fax: + 44 (0) 151 944 1332
e-mail: sales@mast-group.com

Tel: + 49 (0) 4533 2007 0
Fax: + 49 (0) 4533 2007 68
e-mail: mast@mast-diagnostica.deTél. + 33 (0) 322 80 80 67
Fax + 33 (0) 322 80 99 22
e-mail: info@mast-diagnostic.fr

www.mast-group.com