MyGo

FA Q S H E E T

PERFORMANCE MADE EASY

What is the minimum hold time forthermal cycling?

For holds during thermal cycling with no optical acquisitions, the minimum
hold time is 3s. For holds during thermal cycling with optical acquisitions, theminimum hold time is 17s.

Can I use disposables from othervendors?

Yes. However, in order to ensure optimal performance, please ensure that any
3rd party disposables:
1) Are mechanically identical to the standard MyGo disposables, to ensure agood fit in the mounts and correct operation of the heated lid.
2) Provide vapour tight seals when closed.
3) Are optically transparent.
4) Are biocompatible, and free of DNAse and RNAse contamination.

How do I create a template?

All experiments can be used as a template. Click “Open”, then use the “filetype” selection in the file dialog to select the “Template” option, then selectthe file to open as a template.

What quenchers can I use in theinstrument?

For best results use non fluorescent quenchers for example BHQ. If you needto use fluorescent quenchers, e.g. TAMRA, make sure to add the fluorescentquencher to the list of targets in the sample setup to enable spectral
deconvolution.

Can I clean the wells to remove
potential contaminants?

Please contact technical support.

Do I need to optically calibrate theinstrument before using it?

The MyGo Pro comes pre-calibrated for 22 dyes. If the dyes you want toinstall are pre-calibrated then you do not have to run a dye calibration.

When should I generate a dye file?

We would recommend generating dye files if you are using a dye that is notpresent on the list of pre-calibrated dyes. In addition, generating dye files canhelp with multiplexing or other challenging applications.

Can I use dye files across multiple
instruments?

Yes, dye files can be used on different instruments of the same model (i.e.
MyGo Pro).

Page 1/4

Why do some amplification curves
disappear when I change from Fullto Background normalisation in
Auto Quant?

In Full normalisation mode the scale of low intensity amplifications is boosted. InBackground normalization mode the scale of low intensity amplifications
is not boosted.

Can my dye files be used on
historical runs?

Yes. To add new dye files to existing experiments, go to the “Run Settings” taband select the “Display Dye Calibrations” checkbox, then click the “Open”
button to import a dye calibration file.

How will I know if there is a newsoftware version?

The latest version of software is available at www.mygopcr.com

Why have you not made a 96 wellplate MyGo Pro?

We have prioritised data quality over the number of wells. The optical architecture which enables this in the MyGo Pro can not be used for 96 wells, because
it involves fibre optics interfaced to the sides of all wells. In a 96 well block wecannot physically access the sides of all wells with fibre optics.

Do the MyGo instruments conform Yes
to the electrical safety requirementsof IEC 61010 or equivalent and theelectromagnetic compatibility requirements of IEC 61326 or equivalent?
Will my instrument run with empty
tubes?

Yes. The MyGo Pro does not need PCR reagents to be present before performing
a run. However, the MyGo Pro will not run if disposables are not loaded.

Will the MyGo Pro lid lock withoutany tubes in?

No. The MyGo Pro senses the presence of tubes before it locks the lid. If there
are no tubes present, the lid will not lock.

I only want to run one 8 well strip
Yes, however, an empty tube strip should also be loaded so that rows 1 and 4of reactions in my MyGo Pro. Is this are filled.
possible?
Can I change the run settings once a No. Once the run has been started, optical and thermal settings can not be
run has started?
changed.
Can I start a run before setting up
my samples and targets
information?

Yes. You can start an experiment without setting up samples and targets.

Can I change how my samples andtargets are set up while myexperiment is running?

Yes, the MyGo Pro will acquire data from all 120 optical channels in every well at
every acquisition. This means the user can view raw data from all channels in all
wells. You are not restricted to data from a channel chosen at the start of a run.

Do the instruments have built inmemory?

Yes. This memory provides for a data buffer during runs to reduce the risk thatdata is lost due to a poor connection between your PC and the instrument.
Please ensure that you save your data on your PC once your run has finished.

Page 2/4

Can I open the MyGo Pro lid
without switching off the power tothe instrument?

Yes, in the software go to Configuration > MyGo Pro > select your instrument >Open Instrument Lid.

Why don’t the MyGo instruments
have power switches?

All moving parts, including power switches, represent potential points of failure
for instruments. For this reason MyGo instruments do not have powerswitches. MyGo instruments are designed to be left connected to a power
supply at all times. When not in use MyGo instruments enter a low power
standby mode. If you need a power switch for your MyGo instrument, thenthey are available as accessories.

Do I need to use ROX as a passivereference in my MyGo instrument?

Some real-time PCR instruments require the use of ROX as a fluorescentpassive reference to correct for optical artefacts. Generally, modern
instruments do not require the use of passive fluorescent references. TheMyGo instruments do not require the use of ROX as a passive reference.

My LAN is unreliable, how can I
reduce the risk of losing data?

You can run your instrument directly from a USB drive, to which data will be saved.

Where is the threshold for
determining Cq values in
Auto Quant?

Modern methods of determining Cq values are not based on simple thresholds.
Modern methods of Cq determination are model based. Auto Quant fits a modelof a PCR amplification to the fluorescence data observed. This model fit thenenables the estimation of a number of important parameters including Cq values.

Why do some of my amplification
curves drop down before rising in
Auto Quant?

Correct background removal in Auto Quant relies on correct qualitative calling.
Please check that appropriate thresholds are set so that positive amplifications
are called positive.

How many MyGo Pro instruments
can I control from one PC?

You can control up to 200 MyGo Pro instrument from one PC.

Is it normal for the lid of myinstrument to get warm
during use?

Yes.

What does integration time mean?

“Integration time” is the exposure time used by the camera in your MyGo
instrument when measuring fluorescence.

Why is my Cy5 reaction notworking?

Cy5 is a relatively dim reporter, you may need to increase Integration Time toimprove signal to noise with Cy5 labelled hydrolysis probes.

Page 3/4

What kind of computer is required
to run the MyGo Pro software?

The MyGo software runs on windows, Mac and Linux and does not require adedicated PC or laptop.

PC Requirements
The table below outlines minimum computing requirements to run the MyGo software.
Processor

Intel Core i3 2GHz (or equivalent AMD)

Memory8 GB

Hard Disk250 GBLAN

RJ-45 LAN (100MBit)USB

USB 2.0 (if using USB for runs)

Display Resolution

Display Resolution 1280* 1024 of greater

Operating System

Microsoft Windows 8 or 10Version 003
IT-IS Life Science Ltd
Bldg 1000, Units 1201 & 1202, City Gate, Mahon, Cork, Republic of Ireland
contact@itislifescience.com +353 21 601 0371 www.mygopcr.com

MyGo

MyGo Mini
Page 4/4