Follicusan™ DP

Prevents accelerated
Hair Lossconfidential information © CLR - Chemisches Laboratorium Dr. Kurt Richter GmbH

Introduction• Milk based bioactive signaling molecules• DL-Ethylpanthenol• Inositol• Sulfur-rich amino acids(N-acetyl-Cysteine, N-acetyl-Methionine)

Follicusan™ DP

Hair Follicle

Permanent

Basement membrane
Transient
Dermal papilla cells

Follicusan™ DP

Hair Growth Cycle
AnagenFollicusan™ DP

Catagen

TelogenDermal Papilla Cells: essential for hair follicle
development in the hair growth cycle
DP cells are highly specialized fibroblasts
with multiple roles:• scaffold for hair follicle• cell signaling: activation of stem cells;
downward growth of the hair follicle’s
epithelial part• production of surrounding connective
tissueDP cells in Alopecia Androgenetica:• decreased formation of connective
tissue• loss of proliferation capacity

Follicusan™ DP

DP cells in cell culture:
In vitro self-aggregating behavior
under the influence of Follicusan™ DP.
Key to induction of hair follicle
development.

Follicusan™ DP

In vitro Test Results

Stimulation of Protein Biosynthesis
and Cell Vitality120Stimulation of Protein
Biosynthesis and Cell
Vitality (%)
Protein biosynthesis has
been measured by Crystal
Violet assay, cell vitality by
MTT assay on fibroblast
cells related to untreatedcells.

1.6% Follicusan™ DP

3.2% Follicusan™ DP

100806040200Protein Biosynthesis

Follicusan™ DP

Cell Vitality

Stimulation of ECM Synthesisin Fibroblast Cells

ControlFollicusan™ DP

with 6.4% Follicusan™ DP

Follicusan™ DP

In vitro Test Results
Dermal Papilla Cells

Influence on ATP Content

ATP content (%)
Measured 48h afterapplication of Follicusan™
DP in dermal papilla cells
related to the ATP level in
untreated cells (100%).

130120(Method: luciferase/luciferin assay)
110

100
0.4%

0.8%

Follicusan™ DP

Follicusan™ DP

1.6%

Stimulation of Proliferation
Stimulation ofproliferation (%)
Human dermal papilla cells
were grown under optimal
conditions for 72 h.
A BrdU assay was performed
after another 72 h after
application of different
concentrations ofFollicusan™ DP. Results are
related to the proliferation in
untreated cells (100%).

180

170
160
150
140
130120
110
100
0.8%

1.6%Follicusan™ DP

Follicusan™ DP

3.2%

Stimulation ofCollagen Type IV Biosynthesis
Collagen type IVcontent (%)
Human dermal papilla cells
were grown under optimal
conditions for 72 h.
A Type IV Collagen ELISA
was performed after
another 216 h after
application of different
concentrations ofFollicusan™ DP. Results are
related to the Collagencontent in untreated cells(100%).

120115

110

105

100
0.8%

1.6%

Follicusan™ DP

Follicusan™ DP

3.2%

Stimulation of Heparan Sulfate
Proteoglycan Biosynthesis
Synthesis of HeparanSulfate Proteoglycan (%)
Human dermal papilla cells
were grown under optimal
conditions for 72 h.
A Heparan Sulfate
Proteoglycan ELISA wasperformed after another216 h after application ofdifferent concentrations ofFollicusan™ DP. Results are
related to the HeparanSulfate Proteoglycan content
in untreated cells (100%).

130

120

110

100
0.8%

1.6%

Follicusan™ DP

Follicusan™ DP

3.2%

Follicusan™ DP

In vivo Test Results
Trichogram TechniqueInfluence on Alopecia diffusa

Changes in hair
growth cycles (%)

40Anagen
Telogen

20after 3 months of0treatment with aformulation containing
5% Follicusan™ DP.
-20
Trichograms weretaken from volunteers-40(A, B, C, D) to
determine the stage ofhair growth cycle.
-60-80A

Follicusan™ DPBCD

Influence on Alopecia androgenetica
60

Changes in hair
growth cycles (%)
after 3 months oftreatment
withaformulation containing
5% Follicusan™ DP.
Trichograms weretaken from volunteers(A, B, C, D, E) to
determine the stage ofhair growth cycle.

AnagenTelogen

40200-20-40-60-80
A

Follicusan™ DPBCDEFollicusan™ DP

In vivo Test Results
TrichoScan

TrichoScan• A small area of hair is clipped on the scalp
surface (~ 1.8 cm²)
• After 3 days dye is applied to this area
• Grown hair and not yet grown hair are
visualized by high contrast• Anagen hair grows 0.3 mm/ day,telogen hair shows no active growth• Digital image at approx. 20-fold magnification is taken• Total hair number is calculated, as well as
hair thickness and percentage in anagen/telogen ratio

Follicusan™ DP

Influence on Hair Density

Hair density [1/cm²]

TrichoScans were madefrom 24 female
volunteers suffering from
Alopecia androgenetica.
A formulation with 5%
Follicusan™ DP wasapplied on the scalp
twice daily .

200195
190185180

Standard healthy hairdensity ~ 240/cm²

175
170
inital conditions

Follicusan™ DP

3 months

6 months

Influence on Cumulative Hair Thickness

Cumulative hairthickness [mm/cm²]
TrichoScans were made from24 female volunteers sufferingfrom Alopecia androgenetica.
A formulation with 5%
Follicusan™ DP was appliedon the scalp twice daily.
A healthy hair is 0.1 mm thick,with a density of ~240/cm²,
the cumulative hair thickness
is ~24 mm/cm².

1817,51716,51615,515

initial conditions

Follicusan™ DP

3 months

6 months

Influence on Anagen Hair Rate

Anagen rate [%]TrichoScans were madefrom 24 female
volunteers suffering from
Alopecia androgenetica.
A formulation with 5%
Follicusan™ DP wasapplied on the scalp
twice daily.
Standard healthy
percentage of hair inanagen phase ~ 85%.

80757065
initial conditions

Follicusan™ DP

3 months

6 months

Summary• Prevents functional disorders of scalp andfollicle cells through vitalization•Stimulates proliferation and ECM synthesisof human dermal papilla cells•

Stops the progression of Alopecia androgenetica in women

INCI Name:

Water, Alcohol Denat., Panthenyl Ethyl Ether, Milk Protein, Lactose, Inositol, Acetyl
Cysteine, Acetyl Methionine, Sodium Citrate, Citric Acid

Dosage:3.0 - 5.0%

pH-range:> 5.8

Preservation:

preserved with phenoxyethanol and dehydracetic acid (Na salt)

Follicusan™ DP