News Release
25 September 2023
EPS Innovative Medicine (Japan) Co., Ltd.

Having prospect for the development ofsimple, highly effective and safe sublingual vaccines

Our Research Division (Research Center) is conducting research focusing on sublingual vaccines
among vaccines administered orally.

【Three Major Benefits of Sublingual Vaccines】
・Easy taking by oneself

・Wide range of effectiveness from infection prevention to suppression of severity・High level of safety【Summary】① Easy taking by oneself
The current method of vaccination against influenza viruses and COVID-19 is injectionsubcutaneously or intramuscularly, but with sublingual vaccines, tablets are administeredsublingually by oneself, so specialized equipment such as syringes and needles and doctors are notrequired.
Therefore, sublingual vaccines are expected to be widely used in areas with poor medicalenvironments.
② Wide range of effectiveness from infection prevention to suppression of severity
There are various antibodies related to immunity, but the sites that work are different, andantibodies called secretory type IgA are on the mucous membranes and antibodies called IgG arein the blood. Viruses such as influenza viruses and COVID-19 do not directly infect the blood,
but infect the mucous membrane of the upper respiratory tract, so current vaccines that aim toincrease IgG production can prevent severe illness, but are not expected to be effective inpreventing infection itself. In order to prevent infection with these viruses, it is necessary to1 / 3

produce secretory type IgA by sublingual administration, but there are various issues and this has
not been realized.。
However, our Research Center has developed a method to produce secretory type IgA bysublingual administration of a vaccine in a preclinical study using cynomolgus macaques.
Furthermore, we confirmed that IgG in the blood is also produced by this method.
Therefore, sublingual vaccines can be expected to be effective in preventing infection itself while
preventing severe illness after infection, just like current vaccines.
③ High level of safety
Current vaccines have various side effects such as swelling, pain, and fever at the inoculation site
after vaccination, but in the study using cynomolgus macaques with the sublingual vaccine, theseside effects were not confirmed.
The sublingual vaccine is expected to be safer to use than current vaccines.

【Publication and New-release】
The research of our Research Center, which examined the usefulness of the sublingual vaccine
described above, was published in the journal "Biology Methods＆Protocols”.

Title: SARS-CoV-2 sublingual vaccine with RBD antigen and poly(I:C)
adjuvant: Preclinical study in cynomolgus macaques
URL: https://academic.oup.com/biomethods/article/8/1/bpad017/7266774

In addition, this paper was released separately by the University Oxford Press to the whole world.
Title: Rapid acting, oral vaccines are coming soon
URL: https://www.eurekalert.org/news-releases/1000684【About Biology Methods＆Protocols】
Biology Methods and Protocols is an online scientific journal published by Oxford University
Press in UK that publishes papers on biology, biomedicine, preclinical and translational research.

【About EPS Innovative Medicine Co., Ltd.】We are a core company of EPS Creative Health Technology Group Co., Ltd. in the pharmaceutical
discovery business. Our goal is to develop the seeds introduced from academia and the seeds foundin our research department based on the knowledge and know-how cultivated by the EPS Groupover many years, expanding the value of seeds and aims at promoting to Japan and China markets,

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or to license out to the partner enterprises.

Inquiries regarding this matter
EPS Innovative Medicine Co., Ltd.
Research Center Kazuki Tajima
E-mail：tajima.kazuki374@eps.co.jp

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