JWBeStSel-532-CFR
BeStSel CFR ProgramHigh estimation accuracy fora wide range of proteins
including β-structure-rich-proteins such as antibodies.

Compatible with GxP andsatisfying CSV, ER/ES andDI for practicing ALCOA+principle.

Providing eight types ofsecondary structure
information

Secondary structure

Helix 1 – regular α-helix
The middle part of α-helices

Helix 2 – distorted α-helix

Left-twisted

2-2 residues at the ends of α-helices

Anti 1 – left-twisted β-strand
Left-hand twisted antiparallel β-sheet

Anti 2 – relaxed β-strand

RelaxedRelaxed (slightly right-hand twisted) antiparallel β-sheet

Anti 3 – right-twisted β-strand
Right-hand twisted antiparallel β-sheet

Parallel β-strand
Parallel β-sheet

Right-twisted

TurnTurn, as defined by DSSPOthers3,10-helix, π-helix, β-bridge, bend, loop/irregularand invisible regions of the structure

Secondary structure information obtained by BeStSel algorism

Automatic Y-axis conversion and concentration calculationby amino acid sequence and absorption

APPLICATION Secondary structure prediction of therapeutic antibodies
CD spectra of antibodies

This program utilizes ‘BeStSel engine’, which is an algorism developed by Dr. József Kardos and Dr. András Micsonai, et. al. at ELTE
(Eötvös Loránd University, Hungary).

ersthO

rm
TuPa
ra

3

Wavelength/nm

0ti

250

An

240

2230ti

220

An

210

1200

ti

190

An

0102

NRMSD
= 0.02ix

Herceptin®

Experimental
Fitted
SD

el

5000

20H

NRMSD
= 0.01030

1RIABNI™

Experimental
FittedSD

ix

MRE/deg.cm2dmol-1

5000- MabThera®
- RIABNI™
- Herceptin®

40el

NRMSD
= 0.020HMabThera®

Experimental
FittedSD

Fraction(%)

5000

Secondary structure prediction
50

Regulatory Compliance withSpectra Manager™ 2.5 CFR

JASCO Spectra Manager™ 2.5 CFR is designed and developed under ALCOA+ and is a total solution
platform to create accurate and complete data.
WindowsRegister

User Account

Explorer

Access rightC:\

Access to the system is recorded as log.

Administrators

DeleteSpcMngUsers

WorkGroup
FolderRegisterUser AccountWorkgroup 1

Access Level

Access Level

Administrators
Power Users
Users
Limited Users

Managers

DivisionDiion

Workgroup
folder 1

Workgroup
folder 1

Parameter
DataOperatorsvisSystem Policy

DeleteAnalysts

Parameter
Data

Workgroup
folder 2
Parameter
Data

Workgroup 2
Access Level
Managers

Audit Trail(Data log)

DeleteWorkgroup
folder 2

Monitoring

Parameter
Data

Analysts
Operators

Monitoring

Workgroup folder monitoring program
RecordOverview

Solution for data integrity
Enduring Electronic Record
Based on prohibiting function to delete electronic record
and to overwrite save, and also functions for backup andrestore data, electronic records can be saved properly
and can be searched accurately during the data
lifecycle.
User Management
Based on the dual security category ([Access Level] and [WorkGroup]), it is possible to manage different authorization processes
in flexible and independent as total analysis systems,instrumentations and analytical applications.

Audit Trail
It is categorized as 4 different records (system log, application log anddata log), and it is recorded. Each log can be filtered and displayed
under recorded date, user name etc, and it can be exported for audit
trail review.

User Account Security

Computerized System Validation

Based on functions to prevent account duplication or toSpectra Manager™ 2.5 CFR is developed and manufactured properly

protect password, and to prevent unauthorized access,under quality control system adapted ISO 9001, and adapted CSV

administrative authorizations such as system access andstandard.

electronic signature etc., can be managed strictly.

The contents of this material are for reference and illustrative
purposes only. Information, descriptions, and specifications in thispublication are subject to change without notice. JASCO assumes noresponsibility and will not be liable for any errors or omissions
contained herein or for incidental, consequential damages or losses inconnection with the furnishing, performance or use of this material.
ISO-9 0 0 1
ISO-14001

CERTIFIED

Products described herein aredesigned and manufactured by
ISO-9001 and ISO-14001 certified
JASCO Corporation

JASCO CORPORATION

2967-5, Ishikawa-machi, Hachioji-shi, Tokyo 192-8537 Japan
Tel: +81-42-649-5177 Fax:+81-42-646-4515
Web: www.jasco-global.com
DE6607-2311-03