MyGo MiniFA Q S H E E T

PERSONAL PRECISION

What is the minimum hold time forthermal cycling?

For holds during thermal cycling, the minimum hold time is 3 seconds.

Can I use disposables from othervendors?

Yes. However, in order to ensure optimal performance, please ensure that any
3rd party disposables:
1) Are mechanically identical to the standard MyGo disposables, to ensure agood fit in the mounts and correct operation of the heated lid.
2) Provide vapour tight seals when closed.
3) Are optically transparent.
4) Are biocompatible, and free of DNAse and RNAse contamination.

How do I create a template?

All experiments can be used as a template. Click “Open”, then use the “filetype” selection in the file dialog to select the “Template” option, then selectthe file to open as a template.

What quenchers can I use in theinstrument?

For best results use non fluorescent quenchers, for example BHQ.

Can I clean the wells to remove
potential contaminants?

Please contact technical support.

Do I need to optically calibrate theinstrument before using it?

The MyGo Mini comes pre-calibrated for 11 dyes. If the dyes you want toinstall are pre-calibrated then you do not have to run a dye calibration.

When should I generate a dye file?

We would recommend generating dye files if you are using a dye that is notpresent on the list of pre-calibrated dyes. In addition, generating dye files canhelp with multiplexing or other challenging applications.

Can I use dye files across multiple
instruments?

Yes, dye files can be used on different instruments of the same model (i.e.
MyGo Mini S).

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Why do some amplification curves
disappear when I change from Fullto Background normalisation in
Auto Quant?

In Full normalisation mode the scale of low intensity amplifications is boosted. InBackground normalization mode the scale of low intensity amplifications
is not boosted.

Can my dye files be used on
historical runs?

Yes. To add new dye files to existing experiments, go to the “Run Settings” taband select the “Display Dye Calibrations” checkbox, then click the “Open”
button to import a dye calibration file.

How will I know if there is a newsoftware version?

The latest version of software is available at www.mygopcr.com

Do the MyGo instruments conform Yes
to the electrical safety requirementsof IEC 61010 or equivalent and theelectromagnetic compatibility
requirements of IEC 61326 orequivalent?
Will my instrument run withempty tubes?

Yes. The MyGo Mini does not need PCR reagents to be present before
performing a run.

I only want to run one tube in myMyGo Mini. Is this possible?

Yes, please ensure that tubes, with or without reactions are present in wells 1, 5,
9 and 13.

Can I change the run settings once a No. Once the run has been started, optical and thermal settings can not be changed.
run has started?
Can I start a run before setting up
my samples and targets
information?

Yes. You can start an experiment without setting up samples and targets.

Can I change how my samples andtargets are set up while myexperiment is running?

Yes, the MyGo Mini will acquire data from all 3 optical channels in every well at
every acquisition. This means the user can view raw data from all channels in all
wells. You are not restricted to data from a channel chosen at the start of a run.

Do the instruments have built inmemory?

Yes. This memory provides for a data buffer during runs to reduce the risk thatdata is lost due to a poor connection between your PC and the instrument.
Please ensure that you save your data on your PC once your run has finished.

Why don’t the MyGo instruments
have power switches?

All moving parts, including power switches, represent potential points of failure
for instruments. For this reason MyGo instruments do not have powerswitches. MyGo instruments are designed to be left connected to a power
supply at all times. When not in use MyGo instruments enter a low power
standby mode. If you need a power switch for your MyGo instrument, then they
are available as accessories.

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Do I need to use ROX as a passivereference in my MyGo instrument?

Some real-time PCR instruments require the use of ROX as a fluorescentpassive reference to correct for optical artefacts. Generally, moderninstruments do not require the use of passive fluorescent references.
The MyGo instruments do not require the use of ROX as a passive reference.

My LAN is unreliable, how can I
reduce the risk of losing data?

You can run your instrument directly from a USB drive, to which data will be saved.

Where is the threshold for
determining Cq values in Auto
Quant?

Modern methods of determining Cq values are not based on simple thresholds.
Modern methods of Cq determination are model based. Auto Quant fits a modelof a PCR amplification to the fluorescence data observed. This model fit thenenables the estimation of a number of important parameters including Cq values.

Why do some of my amplification
curves drop down before rising inAuto Quant?

Correct background removal in Auto Quant relies on correct qualitative calling.
Please check that appropriate thresholds are set so that positive amplifications
are called positive.

Is it normal for the lid of myinstrument to get warm during use?

Yes.

Can I run the MyGo Mini froma battery?

Yes. A power supply unit, that enables the MyGo Mini to run from a 12V
automotive battery, is available as an accessory.

How many MyGo Mini instruments
can I control from one PC?

You can control up to 400 MyGo Mini instruments from one PC.

What does integration time mean?

“Integration time” is the exposure time used by the camera in your MyGo
instrument when measuring fluorescence.

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What kind of computer is required
to run the MyGo Mini software?

The MyGo software runs on windows, Mac and Linux and does not require adedicated PC or laptop.

PC Requirements
The table below outlines minimum computing requirements to run the MyGo software.
Processor

Intel Core i3 2GHz (or equivalent AMD)

Memory8 GB

Hard Disk250 GB

LAN

RJ-45 LAN (100MBit)USB

USB 2.0 (if using USB for runs)

Display Resolution

Display Resolution 1280* 1024 of greater

Operating System

Microsoft Windows 8 or 10

Version 003
IT-IS Life Science Ltd
Bldg 1000, Units 1201 & 1202, City Gate, Mahon, Cork, Republic of Ireland
contact@itislifescience.com +353 21 601 0371 www.mygopcr.com

MyGo

MyGo Mini
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