THE NEWSLETTER OF ACTIVE MOTIF — October 2005 • volume 6 • number 4

Look inside for theunique products...
... you’ve been
searching for

IN
THIS
ISSUE

2 NEW: CodeQuest™ Brings High-throughput
Bioinformatics to Your Desktop
3 Sensitive Protein Quantiﬁcation Using Fluorescence
4 Novel Dyes for Improved Capillary Electrophoresis

5 Optimized Co-IP of Nuclear Protein Complexes
6 Multiple Tools to Study Kinase Activity
7 Study NFκB Binding at Any DNA SequenceResearch Informatics

NEW: CodeQuest™ Brings High-throughput Bioinformatics to Your Desktop
CodeQuest™ is an accelerated desktop
workstation that makes it easy to buildand process sophisticated informatics pipelines. The system combinescomputer cluster performance withdrag-and-drop pipeline development,
bringing high-throughput bioinformatics
capabilities to your lab in a compact,easy-to-use system.
Complete bioinformatics workstation
CodeQuest™ utilizes the DeCypher
Engine™ accelerator card to drive theincluded BLAST, Smith-Waterman, HMM,
GeneDetective and RepeatMasker
algorithms with the performance of
50-750 CPUs. So, you’ll get answers toyour bioinformatics questions in minutes
or hours, rather than in days or weeks.
Easy-to-use across the lab
The PipeWorks™ user interface enables
you to combine accelerated algorithms
with open source applications into rapid
analysis pipelines (Figure 1). This frees
you from the laborious steps required
to integrate data from multiple web
searches. And, because PipeWorks canbe run from any desktop computer, theentire lab can use CodeQuest to validate
results or annotate sets of genes.
CodeQuest speeds many common
bioinformatics tasks (Table 1), including:• Screening speciﬁc microarray probes• Annotating new genomes• Fast Swiss-Prot protein searches• Identifying microRNAs• Mapping SNPs to a genome
Get more done, and fast!
CodeQuest’s high performance and easeof use makes it possible to complete
projects you hadn’t thought possible.
Visit www.timelogic.com/codequest tolearn how CodeQuest can speed yourresearch informatics activities.

2

CodeQuest includes over
50 informatics analysis
applications

Build workflows bydraging tools, databases, plus input/
output steps to thedesign canvas

Incorporate open source
software for custom
workflow steps

Easily specify multiple
outputs (i.e., savealignments in NCBIBLAST format, and use
sequence-only results as
input for new analyses)

Quick access to yoursequence collections
and databases
Users can view job status
and prioritize pending jobs

Figure 1: The PipeWorks interface simpliﬁed analysis pipeline design with drag-and-drop ease.

Application

AnalysisTime

BLASTX

Annotate 50 BACs against the Human genome

6 hours

T-BLASTN

Compare 4,500 bacterial protein sequences with 192 translated bacterial
genomes2 hours

BLASTN

Screen 26,000 50-mers against the rat genome

1 hour

HMM

Compare 25,000 sequences to PFAM for protein domain classiﬁcation

5 hours

Smith-Waterman Compare 25,000 mouse proteins to 49,000 Human protein sequences

7 hours

GeneDetective

3 hours

Build intron/exon maps for 200 genes to explore alternative splicing

Table 1: What can I accomplish on CodeQuest in a single day?

Uncover gene-to-disease links with AKS
AlmaKnowledgeServer (AKS) is a
powerful literature mining system forelucidating connections between genefunction, disease and related chemicalcompounds. AKS helps you visualize
complex relationships through interactive diagrams, and streamlines yourexploration of CodeQuest results.

Product
CodeQuest™
AlmaKnowledgeServer for CodeQuest™

Your personal informatics supercomputer
CodeQuest includes a powerful dualCPU HP workstation with 2 GB RAM, 800
GB of storage, and a 19” ﬂat panel monitor. And because the system includes
PipeWorks, a DeCypher Engine and all of
our accelerated algorithms, CodeQuest
is a much better value than a computercluster, and is signiﬁcantly easier to use.

Description

Catalog No.

Biocomputing workstation
Literature mining module

75002
75105

Toll Free — 1 877 222 9543

October 2005 • volume 6 • number 4

Fluorescent Protein Quantification

Sensitive Protein Quantiﬁcation Using Fluorescence
Active Motif’s ProStain™ Protein Quantiﬁcation Kit is a simple, sensitive alternative to traditional methods for determining protein levels. Offering high signal
strength, unique spectral properties and robust conjugation, the kit offers limitsof detection that are superior to other ﬂuorescent-based systems, as well as
traditionally used methods, such as the Bradford assay.

“The spectral properties and robust
conjugation of the ProStain
Protein Quantiﬁcation Kit makemeasurement fast and accurate.”
Fast, simple conjugation
Using ProStain is fast and easy; the kit
provides ﬂuorescent dye, dilution buffer
and a protein standard. Simply resuspend the dye and add it to the wells ofa microplate, then add a serial dilutionof the standard protein to produce aStandard Curve, along with your sample.
After a 30-minute, room-temperatureincubation, simply read the ﬂuorescence
to quantify your samples.

www.activemotif.com

absorb (503 nm)

emit (600 nm)

absorb (612 nm)

Fluorescence excitation

1.0

Unbound
Conjugated

0.8

0.6

0.4

0.2

emit (665 nm)

0
400 450 500 550 600 650 700 750 800

Wavelength (nm)Figure 1: Absorption/emission spectra of free/bound dye.
Normalized absorption and emission spectra of free(solid lines) and conjugated dye (dotted lines) in
phosphate buffer of pH 7.2.

Contaminating substances
Unlike many protein determination
methods, the ProStain Protein Quantiﬁcation Kit has been shown to be resistant to the effects of many contaminating agents, such as detergents and salts(Figure 2). However, to achieve the best
results the Standard Curve should be
80

Relative fluorescence

Broad spectral shift for better results
A disadvantage of many protein quantiﬁcation methods, such as Bradford assays,
is that the absorbance spectra of thefree and conjugated forms of the dyepartially overlap. This causes non-linear
protein measurement because free dye
is excited by the same wavelength oflight used to excite the bound dye. In
contrast, the free versus conjugatedabsorbance maxima of the ﬂuorescentdye provided in ProStain are separated
by 108 nm. This means that when conjugated sample is excited at ~500 nm (forexample, at 488 nm), only conjugated
dye is excited. In addition, the emission
intensity of free dye is 50-fold lower
than conjugated dye; taken together,
these features effectively eliminate
background (Figure 1).

70
60
50

BSA alone
BSA + 20 mM NaCl
BSA + 2 mM MgCl2
BSA + 10 mM Tris
BSA + 5 mM (NH4)2SO4
BSA + 0.02% SDS

40
30
20
10
0

1

2

3

4

5

BSA (µg)
Figure 2: Standard Curves of BSA.
Increasing amounts of BSA protein were quantified
using the Fluorescent Protein Quantification Kit in thepresence of a variety of contaminants.

created using the same conditions
as your samples. For example, if yoursamples are prepared in an unusual buffer, then your Standard Curve should be
prepared using the same buffer.
Consistent complex quantiﬁcation
To be effective, a protein quantiﬁcation
method must be consistent when used on
different samples. To show the advantagesof ProStain, it and the Bradford assay wereused to produce BSA Standard Curves
and then to quantify known amounts ofthree different nuclear extracts. Results
obtained with ProStain showed a greater
degree of accuracy than the Bradford
assay, which displayed inconsistent results
between different samples types andconcentrations (Table 1). This type ofinconsistency will impair downstream
analyses performed with your samples as
it will cause signiﬁcant loading errors inyour Westerns, ELISAs and other assays. To
improve the results of your downstream
assays, quantify using ProStain.
HeLa (µg/ml)

ProStain

Bradford

0.5

0.57 (13.8%)

1.39 (177.0%)

1.0

1.18 (17.9%)

0.82 (18.3%)

5.0

5.31 (6.2%)

5.21 (4.2%)

Jurkat (µg/ml)

ProStain

Bradford

0.5

0.46 (7.7%)

0.88 (75.8%)

1.0

0.98 (1.9%)

1.63 (63.2%)

5.0

5.15 (2.9%)

4.84 (3.2%)

MCF-7 (µg/ml)

ProStain

Bradford

0.5

0.51 (1.1%)

0.48 (3.2%)

1.0

1.02 (1.6%)

0.79 (20.7%)

5.0

5.63 (12.7%)

3.52 (29.6%)

Table 1: ProStain and Bradford quantiﬁcation of extracts.
Known amounts of 3 nuclear extracts were assayed intriplicate. The average concentration calculated, and its
error from the known amount quantified, are shown.

Product

FormatCatalog No.

ProStain™ Protein Quantiﬁcation Kit

1000 rxns

15001

Nuclear Extract Kit

100 rxns

40010

3Capillary Electrophoresis

Novel Dyes for Improved Capillary Electrophoresis
Active Motif’s novel CE Dyes are a signiﬁcant improvement over existing
stains used to label proteins prior to capillary electrophoresis (CE). Their easeof use, spectral shift upon conjugation, high quantum yield and ability tomaintain the natural ionic character of labeled protein make the
CE Dyes ideal for use in CE.

added, followed by incubation at either
room temperature or 50ºC dependingon the CE Dye used. Completion of thereaction can be observed by eye due toa change in sample color from blue tored. It’s as simple as that!
Large Stokes Shift minimizes backgroundIn addition to their non-toxic and charge
properties, CE Dyes also undergo a significant change in spectral properties upon
conjugation to amine groups. In their
free state, CE Dyes have very weak ﬂuorescence (quantum yield < 1%), with anabsorbance maximum of 612 nm and anemission maximum of 665 nm. However,upon reaction with a primary amine theabsorbance maximum shortwave-shifts
by more than 100 nm to 503 nm andemits strongly with a quantum yield of
more than 50% at 600 nm. This change
in spectra and quantum yield meansthat the presence of unconjugated label
does not affect downstream analysis andsigniﬁcantly reduces background effects.

Protein analysis using CE
Derivatization of proteins with ﬂuorescent labels prior to CE is commonly used
to overcome the lack of sensitivity whenanalyzing proteins via UV absorbanceor natural ﬂuorescence. In general, theproteins are labeled by attaching dye
molecules to the primary amines oflysine residues. Lysine is used primarily
because it is a relatively abundant aminoacid, and also because lysine residues
are often located at the surface of theprotein. However, as dye labeling is nonuniform, and because proteins usuallycontain many lysine residues, inconsistent labeling of multiple lysine residues
within a single protein is common. If
the dye used affects the overall protein
charge, then a single protein will give rise
to a series of products, each with a different charge. This can cause problems
during separation and other steps.

Non-toxic – for user-friendly labeling
A further disadvantage of commonlyused dyes, such as o-pthaldialdehyde,
naphthalene-2,3-dicarboxaldehyde
and 3-(2-furoyl)quinoline-2-carboxaldehyde, is that derivatization must be
performed in the presence of cyanide.
Use of such a highly toxic compound is
not only undesirable, it creates signiﬁcant disposal problems for the unusedmaterial. In contrast, the CE Dye labelingprocess is simple to perform, non-toxic
and requires only a 30-minute incubation at either room temperature or 50ºC,
depending on the CE Dye used.

One charge – for clear separation
Like other dyes, Active Motif’s CE Dyes
utilize highly abundant and accessible
lysine residues for their attachment
chemistry. However, CE Dyes overcome
the limitation of other dye systems by
maintaining the positive charge of theamine group following dye conjugation(Figure 1). This means that proteins labeled with CE Dyes will not display bandbroadening or require adjusted ioniccharacter calculations to be performed.
The result is that CE Dyes provide ahighly sensitive labeling system thatmaintains the natural charge propertiesof every protein within your sample.

“Labeling proteins for capillary electrophoresis is easy with CE Dyes, while
the large Stokes Shift and charge maintenance help improve your results.”

4

The CE Dye method
Labeling samples with CE Dyes is fastand convenient. The protein sample ofinterest is dissolved in bicarbonate solution (pH unadjusted) and stock CE Dye is

Get better CE results starting now
To improve your CE results, give us a callto order CE Dyes today.

CH3

CH3

Protein(CH2)4NH3O

R

Protein(CH2)4

R

N
CH3

CH3

Figure 1: Labeling with CE Dyes maintains each protein’s natural charge.
Chemical equation depicting the chemistry of CE Dye labeling, which demonstrates the preservation of the positive chargeof the lysine residue of a protein. R stands for the respective chromogenic/fluorogenic group.

Product

FormatCatalog No.

CE Dye 503
CE Dye 540

1 kit
1 kit

15101
15102

Toll Free — 1 877 222 9543

October 2005 • volume 6 • number 4

Co-Immunoprecipitation

Optimized Co-IP of Nuclear Protein Complexes
The Nuclear Complex Co-IP Kit simpliﬁes co-immunoprecipitation studies ofnuclear protein complexes by providing you with optimized reagents for both
nuclear extract preparation and immunoprecipitation.

250

Better Co-IP method and reagentsThe extraction process of the Nuclear
Complex Co-IP Kit was designed toisolate intact protein complexes fromthe nuclear compartments of the cell,
especially complexes that had beenbound to DNA. And, the kit’s versatile
Co-IP reagents offer you the ﬂexibility tovary the stringency of the Co-IP buffer
compositions. This gives you the ability
to study tightly bound or weak protein
complexes with equal ease.

Figure 2: Western blot analysis of the IP’d p33 subunitof the RNA pol II complex.
HeLa cells were grown to confluence on 100 mm plates
and nuclear extracts were prepared using the kit’s
extraction reagents. For IP experiments, the stringencyof the IP High Buffer was increased by supplementing
with NaCl and Detergent. 100 µg of nuclear extract wasused per IP reaction and incubated with either 2 µg
p33 antibody or no antibody. Following the IP, Western
blot analysis was performed using RNA pol II mouse
mAb at 0.1 µg/ml followed by anti-mouse HRP at 1:1000.
Detection of the p33/RNA pol II complex by the RNA
pol II antibody (lane 3) demonstrates that the Co-IP
was successful in maintaining the protein complex. The
input HeLa extract (lane 1) was run as a control for theWestern blot using 0.1 µg/ml RNA pol II.

Maintain the complex
Co-Immunoprecipitation (Co-IP) is often
used to ﬁnd and study protein/protein
interactions. In Co-IP, a ﬁrst antibody
is used to immunoprecipitate a target
antigen, which also co-precipitates any

associated, interacting proteins. Theinteracting proteins are then detectedby Western blot using antibodies targeted against the interacting proteins ofinterest. However, traditional methods
for performing Co-IP are not optimalfor studying complexes of DNA-binding
proteins because these complexes are
frequently disrupted during the extraction process. The immunoprecipitation
process can also be problematic because
many protein complexes are alteredor disrupted by the salt and detergent
composition of the immunoprecipitationbuffers. In response, the protocol and reagents included in the Nuclear Complex
Co-IP Kit were designed to help maintain
nuclear protein complexes during their
extraction and immunoprecipitation.

“The kit improves Co-IP of DNA-binding proteins by providing extraction andimmunoprecipitation components that help maintain nuclear protein complexes.”
Cell nucleus containing the twointeracting proteins of interest

1

2

3

Nuclear extraction

Add antibody
binding beads

Add antibody directed against
one of the proteins of interest

Lane 1. Mol. Weight Marker
Lane 2. Extract
Lane 3. negative IP (no ab)
Lane 4. Co-IP

Wash & collect theimmunoprecipitated proteins

Immunoprecipitate theproteins of interest

Figure 1: Flowchart of the Co-Immunoprecipitation process.

Product

FormatCatalog No.

Nuclear Complex Co-IP Kit

50 rxns54001

www.activemotif.com

2

3

Lane 1 Western blot control
Lane 2 Negative Control (no antibody used in IP)
Lane 3 Co-IP: IP using p33/WB using RNA pol II

Easily modify stringency
The Co-IP Kit contains high and low
stringency IP buffers, as well as salt anddetergent. Addition of salt and detergent is ideal for robust protein/protein
interactions because it reduces background. However, because unstable complexes may not withstand high stringencies, the kit’s convenient format makes it
simple to modify stringency as required
for each particular protein complex.
Advantages• Simple and efﬁcient• Optimized extraction procedure
preserves nuclear protein complexes• Easily alter IP stringency to detect
interactions of varying strengths

4Western blot analysis of the immunoprecipitated proteinsusing an antibody directed against the second protein of interest

1

Order one today
The Nuclear Complex Co-IP Kit offers a
simple, ﬂexible alternative to traditional
immunoprecipitation. To ﬁnd out more
about the Nuclear Complex Co-IP Kit,
visit us at www.activemotif.com.

5

KinasesMultiple Tools to Study Kinase Activity
As interest in kinase activation and its effect on cellular regulation increases,
so does the need for optimized tools to measure phosphorylation and theactivity of kinases. In response, Active Motif has released several new active
recombinant kinase proteins. In addition, the innovative FACE™ Cell-based
ELISA Kits enable the detection of phosphorylated kinases directly within thecell without the need for extracts, gels or blotting.

1.4
1.2
1

Phospho-ERK

0.8

Total-ERK

0.6
0.4
0.2
0

0

1000
100
10
PMA induction (ng/ml)

Figure 1: Colorimetric measurement of phosphorylated
ERK and total ERK.
Murine Macrophage 4/4 cells were cultured in 96-wellplates and serum-starved for 16 hours. Cells were thenstimulated with the indicated amounts of Phorbol 12myristate 13-acetate (PMA) for 10 minutes and fixed. Total
ERK and phospho ERK were each assayed in triplicate
using the phospho-ERK and total-ERK antibodies included in the FACE ERK1/2 Kit. Data was plotted after correction for cell number (performed through use of Crystal
Violet). Note that the induction treatment did not affectthe overall level of total ERK.

NEW - Active Recombinant Kinases
Recombinant AKT1

Recombinant AKT2

Recombinant AKT3

Recombinant CHK2

Recombinant CK2α1

Recombinant CK2α2

Recombinant CK2β

Recombinant eIF2α

Recombinant ERK1

Recombinant ERK2

Recombinant JNK2α1

Recombinant JNK2α2

Recombinant MEK1

Recombinant MKK6 mutant Recombinant p38α

Recombinant PKA

Active proteins for kinase assays
In addition to FACE, Active Motif now
offers an extensive range of active
recombinant kinase proteins. All of thesenew proteins are pre-validated for use
in both Western blotting and activity
assays, so you can feel conﬁdent in yourresults (Figure 2). Give us a call or visit
us at www.activemotif.com for moredetails, including downloadable technical data sheets.

Activity (cpm)

OD450 nmSimpliﬁed phospho-detection
FACE™ (Fast Activated Cell-Based ELISA)
Kits offer you a simple, sensitive and
efﬁcient method for monitoring kinase
phosphorylation (Figure 1). They enable modiﬁcation-speciﬁc analysis to be
performed directly within the cell, which
eliminates the need for time-consuming
extract preparation and Western blotting. In addition, FACE Kits yield resultsthat are more quantitative than Westerns,
further improving your results. All FACE
Kits provide both the phosphorylated andtotal antibody for the kinase of interest,
so you can compare phospho to native
protein levels in the same kit. FACE Kits
are available in both Colorimetric andChemiluminescent formats.

How FACE works
In FACE, cells are cultured in 96-wellplates and stimulated to induce thepathway of choice. Following stimulation, cells are rapidly ﬁxed to preserve
activation-speciﬁc protein modiﬁcations. Each well is then incubated with a
primary antibody speciﬁc for the protein
of interest. Subsequent incubation withsecondary HRP-conjugated antibody anddeveloping solution provides a colorimetric or chemiluminescent readout.

160,000
140,000
120,000
100,000
80,00060,00040,00020,000
0

0

10

20

30

40

50

ng/assayFigure 2: ERK1 Kinase Assay.
Varying amounts of the ERK1 enzyme were used in a kinase
assay to phosphorylate MBP (Myelin Basic Protein).

Recombinant PKCαFACE™ Product Line
FACE™ AKTFACE™ ATF-2

FACE™ Bad

FACE™ c-Jun (S63)

FACE™ c-Jun (S73)

FACE™ c-Src

FACE™ EGFR (Y992)

FACE™ EGFR (Y1173)

FACE™ ErbB-2 (Y877)

FACE™ ErbB-2 (Y1248)

FACE™ ERK1/2

FACE™ FAKFACE™ FKHR (FOXO1)FACE™ GSK3β

FACE™ JAK1FACE™ JNKFACE™ MEK1/2

FACE™ NFκB Proﬁler

FACE™ p38

FACE™ PI3 Kinase p85

FACE™ STAT2

FACE™ STAT4

FACE™ STAT6

6

All your kinase needs
Active Motif’s broad line of products forstudying both kinase phosphorylation
and activity provide you with the tools
you need. To learn more, please returnthe enclosed reply card or visit us atwww.activemotif.com.

Toll Free — 1 877 222 9543

October 2005 • volume 6 • number 4

D N A - b i n d i n gK iEnLaI sS eA s

Study NFκB Binding at Any DNA Sequence

Flexi Kits give you options
Flexi Kits are the latest version of ouroriginal TransAM Kits, which are themethod of choice for studying transcription factor binding activity. Original
TransAM Kits offer a non-radioactive
alternative to gelshift assays by providing a 96-well plate that is pre-coatedwith the consensus-binding site for thefactor of interest. While this is convenient for measuring binding activity at aconsensus site, it does not enable you to
study alternative sites. In contrast, Flexi
Kits provide all of our proven TransAM
reagents (antibodies, controls and reaction buffers), but give you the ﬂexibility
to test the relative binding of NFκB at
various promoters, or at mutated sites.

How do TransAM Flexi Kits work?
In Flexi Kits, you ﬁrst design biotinylated
oligos or PCR products that contain thebinding sites you wish to study. Eacholigo is then incubated with nuclear
extract that has been treated to activate
the NFκB member you wish to study.
The extract/oligo mixture is thentransferred to a 96-well, streptavidincoated plate, which captures the bound,biotinylated oligonucleotide. A primary
antibody speciﬁc for activated NFκB
is added, followed by HRP-conjugatedsecondary antibody and developing
reagent. The levels of DNA-bound NFκB
are then read on a spectrophotometer,
which provides a quantitative readout of
NFκB binding at your site (Figure 1).

“TransAM Flexi Kits make it possible to quickly quantify the binding of
NFκB family members at any binding site you choose.”
Add primary
antibody

Add the extract-DNA
cocktail to plate

1 hr.

Add anti-IgG
HRP conjugate

1 hr.

Incubate biotinylated DNA with cell extractcontaining activated transcription factor

Add developing
and stop solution

1 hr.

Streptavidin
coated plate

Figure 1: Flowchart of the TransAM Flexi procedure.

Product

FormatCatalog No.

TransAM™ Flexi NFκB Family

2 x 96-well plate

43298

TransAM™ Flexi NFκB p50

1 x 96-well plate

41098

TransAM™ Flexi NFκB p65

1 x 96-well plate

40098

www.activemotif.com

TransAM Flexi NFκB advantages• Monitor NFκB binding at any site
• Sensitive & quantitative• Results in less than 5 hours• Assay cell or tissue samples
NFκB activation (OD450 nm)

Active Motif’s TransAM™ Flexi NFκB Kits provide a fast, quantitative method
for proﬁling the binding of the NFκB Family members p65, p50, p52, c-Rel
and RelB at any DNA-binding site you choose.

0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0

Untreated
Treatedconsensus
control

Igκ

IL-8

uPA

50 bp
5´

TF Binding site

3´BIgκ - GGAAAGTCCC
IL-8 - GGAAATTCCA
uPA - GTACTTTCCC

Figure 2: Measuring NFκB p65 binding at different sites.
Five µg of nuclear extract from untreated and TNF-αtreated HeLa cells were used to assay the binding affinity
of NFκB p65 for four different biotinylated 50-mer
oligos. Each of the 3 test oligos (Igκ, IL-8 and uPA) contained the wild-type binding site of a promoter regulated
by NFκB. These sites were compared to the consensusbinding site control provided in TransAM Flexi Kits.

Compare binding at multiple sites
Flexi Kits make it easy to quantitate thebinding of NFκB family members at any
binding site. To demonstrate, three different oligos, each containing different native
NFκB binding sites and known to havedifferent afﬁnities for activated NFκB p65,
were synthesized. Following induction by
TNF-α, the relative binding of activated
p65 at each site was measured. The results
conﬁrm that NFκB p65 had its weakest
afﬁnity for the IL-8 promoter (Figure 2).
Get Flexi today
TransAM Flexi Kits make it fast and easy
to study binding of NFκB members (p50,p65, p52, c-Rel and RelB) at any sequence
you choose. For complete details, visit us
at www.activemotif.com/transam.

7

Active Motif Ofﬁces
U.S.

EUROPE

JAPAN

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Carlsbad, CA 92008
Toll Free: 877 222 9543
Direct: 760 431 1263
Fax: 760 431 1351
Email: sales@activemotif.com

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Other countries, Direct: +32 (0)2 653 0001
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Email: eurotech@activemotif.com

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Direct: +81 (0)3 5225 3638
Fax: +81 (0)3 5261 8733
Email: japantech@activemotif.com

Distribution Network
Active Motif is represented in the
countries listed to the right throughour network of distributors. For
additional contact and ordering
information, please visit the
distributor page on our website:www.activemotif.com/distributors.

8

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