Building 3

Low Temperature Facility

3rd Floor

The facility is equipped with laboratories andequipment for separation and purification offunctional substances such as proteins andpolysaccharides from living bodies using thelow-temperature environment. It also has

Low
Temperature
Facility

Laser and High-Tech Masticatory
Function
Equipment Facility
Research Facility

laboratories and equipment in which dentalEVmaterials can be examined under theenvironment at extremely low temperature
or constant temperature and humidity.

A two-dimensional electrophoresis apparatus
for protein analysis is also equipped.

Ultralow temperature tank R-100LM

Medical freezer MDF-U442 (SANYO)

Freeze dryer DC800 (YAMATO)

PhotographProcessing
FacilityLaser and High-Tech Equipment Facility

Building 3

3rd Floor

Laser is one of the most important devices

that humans invented in this century. A laser
beam has three characteristics: goodmonochromaticity, good directivity, and highpower. In quantum mechanics, light is aLow
Temperature
Facilitycollection of particles (quantum) called

Laser and High-Tech Masticatory
Function
Equipment Facility
Research Facility

PhotographProcessing
FacilityEV

photons, and the uniformity of the energyof a photon leads to directivity, and a laser
beam containing a very large number ofphotons leads to high power. It is widely applied in the fields of dentistry and medicine such as:

surface treatment of dental enamel, giving acid resistance, removal of dentin caries, reduction ofocclusal pain, laser scalpel, treatment for retinal detachment, LASIK (ophthalmology), and removalof lentigines/melanin spots (dermatology).

This institution is currently used as an open laboratory for experimentation by faculty members,
postgraduates, and undergraduates of School of Dentistry and Faculty of Health Sciences.

Microscopic electrophysiological system patch-clamp (Nikon)
A device for examining ion channels such as potential difference
and conductivity by attaching a cell membrane of a specific cellto the tip of a fine glass electrode with a pore under a microscope.

Open laboratory

Masticatory Function Research Facility

Building 3

3rd Floor

Laboratory equipment for various research

related to mastication is installed. In particular,a shield room equipped with devices formeasuring and recording biopotentials(e.g. jaw and facial muscle activity) during
mastication and a measuring instrument

Low
Temperature
Facility

Laser and High-Tech Masticatory
Function
Equipment Facility
Research Facility

PhotographProcessing
Facility

EVfor plantar pressure distribution are used
for the study of the relationship between

masticatory movement and systemic function.

Shield room
Electromagnetic shielding room to improve the measurement
accuracy of the biological monitor.

Pressure distribution measurement system I-SCAN, T-SCAN (NITTA)
The pressure distribution is measured using an ultra-thin film sensor.

Transcranial magnetic stimulator Magstim2002 (Miyuki Giken)
A device that uses a single magnetic stimulation pulse to induce
tissue current in the body and stimulates nerves and muscle tissue.

Photograph-Processing Facility

Building 3

3rd Floor

There is a device that can digitalize analogous

images obtained from various microscopes withthe latest devices necessary for processes suchas image monitoring with good reproducibility,
image processing, image analysis, stereo

image construction, and image output. WithLow
Temperature
Facility

Laser and High-Tech Masticatory
Function
Equipment Facility
Research Facility

PhotographProcessingFacilityEVthe three-dimensional bone quality/bonemorphology measurement software, it cananalyze the three-dimensional network

structure of cancellous bone based on bone tomographic images. Also, a large printer enables
the preparation through to the materials for the publication of papers, etc.

Bone quality/bone morphology measurement software
TRI/3D-Bon (RATOC Systems)
It is possible to analyze images of bone quality, bone density,bone morphology, etc. from images using permanently installedmicro-focus X-ray CT in the morphological research facility.

Large printer MAXART PX-H10000 (EPSON)
It can print a poster up to approx. 1.12 m * 2.5 m.

Microscope image input device Mac (Olympus)
The optical microscope images can be imported into a computerwith a high vision still image camera.

Building 3

Tissue Culture Facility

2nd Floor

The facility is equipped with equipment mainlyfor cell culture. There are 3 clean benches and
13 CO2 incubators, which are mainly used for
cultivation of human/animal-derived cells.
Instruments for analysis are also installed

TissueCultureFacilitysuch as an inverted phase contrast

Analytical
Instrument
Facility

BiomaterialsResearchFacility

EV

Director's OﬃceOﬃce
MeetingRoom, etc.

microscope for observation of cultured

cells, a photographing device for observationof cells, and a confocal laser microscope.

Clean bench (DALTON)
Equipment to prevent contamination of dust and environmental
microorganisms and perform aseptic technique during cell culture

CO2 Incubator (YAMATO)
Device that adjusts the internal temperature and CO2
temperature for cell culture

Confocal laser scanning microscope
LSM 700 2ch UGRB (Carl Zeiss)
Three-dimensional images can be created by a combination offluorescence measurements and optical sectioning of specimens
in a confocal fashion with a laser beamBuilding 3

Analytical Instrument Facility

2nd Floor

This facility meets the needs to obtain knowledge
about the type of substances and its content,chemical structure, and existential state.

Equipped with biological molecular interaction
analyzers, X-ray diffractometer, imagingsystem, real-time PCR system, highTissueCultureFacility

Analytical
Instrument
Facility

Biomaterials
ResearchFacility

EV

Director's Oﬃce
Oﬃce
MeetingRoom, etc.

performance liquid chromatography,

spectrophotometer, ultracentrifuge, etc.

autoMACS Pro Separator (Miltenyi Biotec)
A computer-controlled automated magnetic cell separation device
using MACS technology, which is fully automated from magnetic
labeling to separation of samples. It allows high speed and highpurity continuous cell separation up to 6 samples.

Real-time PCR system Step One Plus
Device to measure real-time polymerase chain reaction (PCR)
amplification. It is used for gene expression, SNPs typing baseinformation, genetic recombinant food testing, detection ofviruses and pathogens, and analysis of the number of copies
of introduced genes.

FACSVerseTM Flow cytometer (Beckton Dickinson)
A cell analyzer that uses up to 8 parameters with 3 types of lasers(488 nm/640 nm/405 nm). Apoptosis, cell cycle, cell proliferation,and cytokine detection are possible by using a special reagent kit.
There are also FACSCaliburTM and FACSAriaTM cell sorter in thefacility.

Building 3Biomaterials Research Facility

2nd Floor

The facility has equipment to study the physical properties of materials. A universal testingmachine, Servo Pulsar, and multiple microplate reader are installed.

TissueCultureFacility

AnalyticalInstrument
Facility

Biomaterials
ResearchFacility

EV

Director's Oﬃce
Oﬃce
MeetingRoom, etc.

Universal testing machine Autograph AGS-X (SHIMADZU)
Device to measure compressive strength, tensile strength,
shear strength of experimental samples

Servo Pulsar EHF-F1 (SHIMADZU)
Device to investigate mechanical properties such as tensile
strength, bending, fatigue, creep, and destructive elasticity
by applying load to samples such as uniaxial tensile strength,compression, and repetition

Multi-microplate reader SpectraMax Plus
Capable of detecting visible to ultraviolet absorption, fluorescenceintensity, fluorescence polarization, time-resolved fluorescenceand luminescence. It is used for DNA/RNA/protein assay andpurity test, cytotoxicity test, etc.

Building 3

Biomaterials Research Facility

1st Floor

Analytical devices to examine microstructures

such as biological tissues and dental materials

and devices for the preparation of their samples
are installed. Main instruments include

precision analyzers such as transmission
electron microscope, scanning electron

Biomaterials Research Facility

CentralEquipment
RoomSeminar
RoomLecture
Rooms for
GraduateSchoolEVmicroscope, micro-focus X-ray CT, andHS all-in-one fluorescence microscope,

as well as a microtome for sample preparation,

critical point dryer, freeze dryer, ion sputtering device, and vacuum vapor deposition apparatus.

A scanning photoelectron spectrometer PHI X-tool (ULVAC-PHI) was installed with the subsidy for
improvement of research facilities provided by the Ministry of Education, Culture, Sports, Scienceand Technology in 2013.

Microfocus X-ray CT SKYSCAN1275
It can nondestructively examine the distribution density inside thesubstances by X-ray irradiation and take CT images.

Field emission-type scanning electron microscope S-4800 (Hitachi)High resolution can be observed at low accelerated voltages withhigh resolution FE-SEM. Super ExB to suppress charge-up oredge effect is mounted.

Scanning electron spectrometer PHI X-tool (ULVAC-PHI)
Photo-electron spectra released from a surface at a depth ofabout 10 nm are measured and information on surface chemical
analysis is obtained such as surface composition and chemicalbonding status.

Building 3

Biomaterials Research Facility

1st Floor

The facility is equipped with equipment to
prepare specimens for use in research.

Biomaterials Research Facility

CentralEquipment
RoomSeminar
RoomLecture
Rooms forGraduate
SchoolEV

Distilled water/pure water purification machine (ADVANTEC)
Device to purify distilled water, ion-exchanged water,and pure water used for research.

Crash ice making machine CM-350AWE (Hoshizaki)
Crush ice machine for cooling samples, etc. in research.

Dry ice machine (ISIS)
Device to prepare dry ice for freezing samples and coolingsamples during transportation

Building 3

Dental biology facility

Basement Floor

It is equipped with a gene introduction device,nucleic acid extraction device, Sanger sequencer,
454 GS Junior System (the next generation

sequencer), etc. to conduct basic dental medicine
research, mainly genetic analysis. A cell sorter

EVAnimals Facility

Dental biologyfacility

to analyze gene expression and to examine therelationship with functional differentiation of cells
is installed in the analytical equipment facility.

Sequence system GSjunior (Roche Diagnostics)
A bench-top size sequencer that enables analysis of bacterial
genome information and human gene polymorphism/expression
analysis.

Spiral plater EDDY JET2 (IUL)
Automatic plater capable of sucking a sample to a spiral smear onan agar plate by using a dedicated disposable microsyringe.

High-speed cooling centrifuge himac CR22G (HITACHI)
High-speed cooling centrifuge capable of using from 15 mL ofconical tube to 1.5 L dedicated bottle by replacing rotors.

Building 3

Animals Facility

Basement Floor

Facility where laboratory animals are housed

EV

Animals Facility

Dental biology
facility

About animal experiments
Animal experiments must be conducted not only following the scientific methods ingeneral experiments but also in a way in which the life of laboratory animals is respected and unnecessary pain is not given. Matters related to animal welfare andanimal management are specified in the "Act on Welfare and Management of Animals"(the Animal Welfare Act). The Animal Welfare Act specifies 3Rs in animal experiments(Reduction, Refinement, and Replacement) as "consideration when animals are used
for scientific purposes."

1. Relevant rules“Standards relating to the Care and Keeping and Reducing Pain of Laboratory Animals”
by the Ministry of the Environment and the "Rules for animal experiments in Osaka Dental
University" are stipulated in accordance with the "Basic guidelines for conduct of animalexperiments in research institutions, etc." notified by the public notice of the Ministry ofEducation, Culture, Sports, Science and Technology, and show the points to be observedin planning and conducting animal experiments.

2. Self-inspection/evaluation report on animal experiments
Based on the public notice of the Ministry of Education, Culture, Sports, Science andTechnology, "self-inspection and evaluation on animal experiments" are conducted.

3. Cross-validation program for animal experiments
External evaluation is performed for "self-inspection and evaluation on animal experiments."