Product Data SheetdSurf 3 x 4mL of 3M™ Novec™ 7500 Engineered Fluid with 2% dSURF

DR-RE-SU-12

dSurf 30mL of 3M™ Novec™ 7500 Engineered Fluid with 2% dSURF

DR-RE-SU-30

dOil 120mL 3M™ Novec™ 7500 Engineered Fluid

DR-RE-SU-A1

Ver. 10.2018 + 9.2019

Table of Contents

1. Overview…………………………………………………………...…3
2. Benefits …………………………………………………..……………3
3. How to use dSURF…………………………………………….……...4
4. Contact information……….………………………........................4dSURF product data sheet, Fluigent
Local distributor in Japan: ASICON Tokyo Ltd.

2Overview
Droplet microfluidics has emerged as powerful tool for high-throughput
biomolecular analysis. A water in oil droplet is used as a picoliter reactor inwhich a single object such as a cell, a DNA strand, a protein/peptide can be
encapsulated with various reagents. In 2015, the McCarroll lab* at Harvard
opened a new path with the Drop-seq method for studying RNA expression
genome-wide in thousands of cells in a run. Today, several dPCR systems arecommercially available. Fluorinated oils have shown several advantages
compare to other carrier fluids such as mineral oils. Their low viscosity allows foreasy handling in microfluidic systems without need of high-pressure pumps. Also,
they show a better PDMS compatibility due to minimum swelling. They are also
more adapted to biochemical experiments due to low organic compoundtransfer drop to drop and they have shown better biocompatibility in long termin droplet cell culture experiments. To maintain stable droplets in fluorinated oil
the use of fluorosurfactant is mandatory.

dSURF is a new generation of fluorosurfactant providing highly reliable droplet
production and stability even under strong conditions such dPCR temperature
cycles or long-term cell culture experiments. dSURF has been validated withseveral dyes showing limited diffusion outside the droplets. Its biocompatibility
has been qualified through in droplet bacteria, yeast and mammalian cellgrowth.
* http://mccarrolllab.org/dropseq/dSURF product data sheet, Fluigent
Local distributor in Japan: ASICON Tokyo Ltd.

3Benefits} Highly reliable droplet formation and production from 20µm to 100µmdiameter. Compatible with droplet generation chips by microfluidic
ChipShop.
} FAM™, HEX™, VIC® and EvaGreen® based dPCR validated.
} Suited for long-term cell culture experiments} High purity

How to use dSURF
dSURF comes diluted at 2% in 3M™ Novec™ 7500 oil. Depending on theapplication, dSURF can be used directly at 2% concentration. However, forother applications this concentration can be reduced to 0,5% or 1%. Fluigent
provides pure fluorinated Novec™ 7500 oil (dOIL, part number: DR-RE-SU-P1) forsuch dilutions. According to our applications notes and customer’s procedures,
Fluigent recommends using the 2% concentration for dPCR experiments anddown to 0.5% -1% concentration for cell culture experiments. Each experimentbeing different, it is recommended to perform preliminary testing to determine
the best concentration.
Visit www.asicon-tokyo.com/cs07.php suggesting Flow EZ pressure controllers,
P-Caps and thermoplastic polymer chips by microfluidic ChipShop for easy
droplet generation.

Contact information
Fluigent has worldwide exclusivity for selling dSURF.
⽇本代理店 / Your local contact in Japan:
ASICON Tokyo Ltd.
asicon-tokyo@asicon-tokyo.com
www.asicon-tokyo.com

dSURF product data sheet, Fluigent
Local distributor in Japan: ASICON Tokyo Ltd.

4