Dharmacon™ Reagents

Edit-R™ All-in-one lentiviral sgRNA controls
Requirements

B. Edit-R All-in-one lentiviral sgRNA positive controls

25 μL × 2 (50 μL total) of 1 × 107 TU/mL or greater

Itemized list of contents

Lentiviral Particles (net quantity 0.025 mL per vial),
Dry Ice (net quantity 15 kg)
A. Edit-R All-in-one lentiviral sgRNA non-targeting controls (NTC)Cat #Item description

Lot number_Titer*

Volume Qty

VSGC11954

Edit-R All-in-one LentiviralsgRNA mCMV Non-targeting
Control #1

V21110502_1.34 × 108 TU/mL
V22072903_2.37 × 107 TU/mL
V23050503_2.29 × 108 TU/mL
V24022303_1.56 x 108 TU/mL25 μL

2

VSGC12059

Edit-R All-in-one LentiviralsgRNA mCMV-EGFP
Non-targeting Control #1

V22102002_1.04 × 108 TU/mL
V23051204_5.84 × 107 TU/mL
V24012706_3.96 × 107 TU/mL

25 μL

2

VSGC11964

Edit-R All-in-one LentiviralsgRNA hEF1a Non-targeting
Control #1

V23011303_1.38 × 108 TU/mL
V23031608_2.01 x 108 TU/mL
V23063003_6.86 x 107 TU/mL
V24011201_4.29 x 107 TU/mL25 μL

2

Edit-R All-in-one LentiviralsgRNA hEF1a-EGFP
Non-targeting Control #1

V22060306_4.67 × 108 TU/mL
V22121503_8.24 × 107 TU/mL
V23051204_5.58 × 107 TU/mL
V23060205_1.69 × 107 TU/mL
V23072801_1.93 × 107 TU/mL
V24042603_3.40 × 107 TU/mL

25 μL

VSGC11966

Edit-R All-in-One LentiviralsgRNA hEF1a Non-Targeting
Control #2

V20081302_1.30 × 108 TU/mL
V21012802_9.55 × 107 TU/mL
V22100703_2.91 × 107 TU/mL

25 μL

2

VSGC11956

Edit-R All-in-One LentiviralsgRNA mCMV Non-Targeting
Control #2

V23050504_2.87 × 108 TU/mL25 μL

2

VSGC12063

Cat #Item description

Lot number_Titer*

Volume Qty

VSGH11974

Edit-R Human All-in-one
DNMT3Bb mCMV LentiviralsgRNA Positive Control

V18102602_5.10 × 107 TU/mL

25 μL

2

VSGH12099

Edit-R Human All-in-one
DNMT3B mCMV-EGFP
Lentiviral sgRNA PositiveControl

V22091501_5.78 × 107 TU/mL
V22120206_1.80 × 107 TU/mL
V22120904_3.04 × 107 TU/mL25 μL

2

VSGH11976

Edit-R Human All-in-oneDNMT3B hEF1a LentiviralsgRNA Positive Control

V19090603_7.44 × 107 TU/mL25 μL

2

VSGH12103

Edit-R Human All-in-one
DNMT3B hEF1a-EGFP
Lentiviral sgRNA PositiveControl

V21081904_2.04 × 107 TU/mL
V24021502_2.46 × 107 TU/mL

25 μL

2

VSGH11978

Edit-R Human All-in-one PPIBmCMV Lentiviral sgRNA
Positive Control

V18102603_4.23 × 107 TU/mL

25 μL

2

VSGH12107

Edit-R Human All-in-one PPIB
mCMV-EGFP Lentiviral sgRNA
Positive Control

V21110404_1.57 × 107 TU/mLV24020802_2.59 × 107 TU/mL25 μL

2

VSGH11980

Edit-R Human All-in-one PPIBhEF1a Lentiviral sgRNA
Positive Control

V19090506_8.87 × 107 TU/mL25 μL

2

VSGH12111

Edit-R Human All-in-one PPIBhEF1a-EGFP Lentiviral sgRNA
Positive Control

V22100705_1.63 × 107 TU/mL
V23050306_1.04 × 107 TU/mL25 μL

22Shipping and storage

Store at –80 °C. Edit-R All-in-one Lentiviral sgRNA Controls are shippedon dry ice and should be stored in a –80 °C freezer upon arrival.
Edit-R lentiviral particles will remain stable for at least one year without
any appreciable loss in titer. When ready to use, thaw the viral particles
on ice. The viral titers indicated above for each sample are determinedfrom an aliquot that has been through one freeze-thaw. The vials in thispackage have not been thawed; therefore, the indicated titers areaccurate for the ﬁrst thaw. However, every subsequent freeze-thaw may
result in a decrease in titer of two to ﬁve-fold. Any viral particles
remaining after the ﬁrst thaw can be aliquoted into smaller volumes andstored at –80 °C.

Classiﬁcation of Edit-R lentiviral sgRNA controls
Edit-R viral particles are not capable of causing permanent disability,
life-threatening or fatal disease in otherwise healthy humans or animals,
and therefore are not classiﬁed as infectious substances under IATA andDOT guidelines.

Description of goods

Replication incompetent viral-like particles of human, mouse, and/or rat
origin; intended use is for research purposes only.
Proper packaging and labeling of shipments will be utilized to ensure
safe and timely transit of Edit-R sgRNA products.

The information provided in this document is valid for the specified lot number and date of analysis. This information is for reference purposes only and does
not constitute a warranty or guarantee of the product’s suitability for any specific use. Revvity, Inc., its subsidiaries, and/or affiliates (collectively, “Revvity”) do
not assume any liability for any errors or damages arising from the use of this document or the product described herein. REVVITY EXPRESSLY DISCLAIMS ALL
WARRANTIES, INCLUDING WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, REGARDLESS OF WHETHER ORAL OR WRITTEN,EXPRESS OR IMPLIED, ALLEGEDLY ARISING FROM ANY USAGE OF ANY TRADE OR ANY COURSE OF DEALING, IN CONNECTION WITH THE USE OF
INFORMATION CONTAINED HEREIN OR THE PRODUCT ITSELF.

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