Accuracy
elevated.

Cellometer™ Ascend™ automated cell counterCellometer Ascend automated cell counter• Introduction• Features and benefits• Technology• Reagents and consumables• Software

We’ve raised
the bar forcell counting.

An integrated, high resolution touchscreen
elevates the user experience.

• Performance• Specifications• Support• Contact us

Introducing the Cellometer™ Ascend™ automated cellcounter, the newest innovation in our renowned, trusted
portfolio of cell counting instruments.
Packed with advanced functionalities including autofocus,
sample slide detection, and automatic result analysis, theCellometer Ascend accelerates your lab workflow, helping
to mitigate human error... all while ensuring a consistent,
standardized cell count.
With its revolutionary design, the Cellometer Ascend haselevated accuracy and reliability in cell counting. Plus, its
user-friendly Matrix™ software incorporates an automatedand sophisticated image analysis workflow that delivers
reliable results in a matter of seconds.

For research use only.
Not for use in diagnostic procedures.
2 |Cellometer Ascend automated cell counter

FEATURES AND BENEFITS• Introduction

Heightened functionality accelerates
your research workflow.

• Features and benefits• Technology• Reagents and consumables• Software• Performance• Specifications• Support• Contact us• Cell counts and viability information: dual fluorescence and brightfieldimaging assist with increasing accuracy for nuclei and cell counting• Fast results: size, count, concentration, and viability calculationsin <60 seconds• Count up to eight samples at a time: choose between a three- or
eight-chamber slide• Multiple fields of view: capture up to eight images per sample
to increase the accuracy of counts• Analysis of various sample types: including cultured cell lines,PBMCs, primary cells, whole blood, and bone marrow• Small sample volume: as little as 10 µl of cell sample needed• Favorites feature: customize experiment settings andsave commonly used assays• Built-in predefined protocols: quickly analyze viability,apoptosis, and transfection efficiency• Pre-loaded cell types: includes saved parameters for over
400 cell types• 21 CFR Part 11 ready: optional add-on that includes
an audit trail, user access control, and digital signature

For research use only.
Not for use in diagnostic procedures.
3 |Cellometer Ascend automated cell counter

TECHNOLOGY• Introduction• Features and benefits

Accuracy elevated.

• Technology• Reagents and consumables• Software• Performance• Specifications• Support• Contact us

Cell counting is fundamental in cell biology laboratories, yet can be a tedious timeconsuming task, prone to the inefficiencies and inaccuracies of manual methods.
Accuracy is paramount for maintaining the integrity of downstream workflows,
especially within cell therapy research.
The Cellometer Ascend distinguishes itself for its exceptional performance:• Proprietary image-based cell identification algorithms• A choice of two auto-focus methods: slide* or image-based focus, to get thebest results for your particular sample and cell type
• Bi-directional (x, y) stage movement to capture themaximum sample volume, especially for samples with low
cell concentration* Slide-based focusing uses a target
on the slide to provide positionalverification and create consistentfocus between samples.

For research use only.
Not for use in diagnostic procedures.
4 |Cellometer Ascend automated cell counter

TECHNOLOGY• Introduction• Features and benefits• Technology• Reagents and consumables• Software• Performance• Specifications• Support• Contact us

Speed elevated.
A key aspect of the Cellometer Ascend’s performance is its counting
speed. Compared to manual counting, it’s about 10 times faster.
• Sample slides are detected automatically: counting begins as soon
as a slide is inserted• Samples are illuminated by high power LEDs minimizing theexposure time and generating fast results• Focusing is automatic, with a choice of two methods to best suit
your sample

For research use only.
Not for use in diagnostic procedures.
5 |Cellometer Ascend automated cell counter

TECHNOLOGY• Introduction• Features and benefits• Technology• Reagents and consumables• Software• Performance• Specifications• Support• Contact us

Technology elevated.
The Cellometer Ascend is the pinnacle for a simplified user-experience. Its
integrated touchscreen and small footprint bring advanced cell counting
capabilities directly onto your benchtop.
• Bright, high-resolution viewing• Flexible screen angle• Glove-friendly touchscreen• Pinch to zoom function• On-screen keyboard for custom naming• Integrated USB/USB-C ports• Wi-Fi or ethernet enabled for network data storage

For research use only.
Not for use in diagnostic procedures.
6 |Cellometer Ascend automated cell counter

REAGENTS AND CONSUMABLES• Introduction• Features and benefits

Better by design.

• Technology• Software

The Cellometer Ascend is accompanied by dedicatedreagents and consumables designed to work together togive you optimal results.

• Performance

Sampling elevated• Specifications

The Cellometer Ascend’s single-use counting slides,with either 3 or 8 counting chambers, bring throughput
flexibility to the benchtop.

• Reagents and consumables• Support• Contact us

Advantages of Cellometer Ascend slides:• Run up to 8 samples at once
• Preform multiple replicates within a single slide• Disposable slides for infectious sample safety control• Mitigated cross-contamination risk
• No clogging• Ideal for fragile cell types such as hepatocytes• Wide dynamic range for cell concentrations• Minimal sample volume of 10 µl for the 8-chamber slide• Composed of shatterproof plastic materials• Integrated autofocus feature on the three-chamber slide• Three-chamber slide is optimized for low
concentration samples

For research use only.
Not for use in diagnostic procedures.
7 |Cellometer Ascend automated cell counter• Introduction

Optimized reagents and consumables analysis• Features and benefits

The dual-fluorescence stain combination AOPI (acridine orange – propidium iodide) is the preferred
method for viability analysis of primary cell samples that may contain debris or red blood cell contamination.

• Technology• Reagents and consumables• Software

The instrument automatically reports live/dead cell number, live/deadcell concentration, mean diameter, and percent viability.

• Performance

In addition, other wash-free and incubation-free assays compliment
standard viability measurements:• Specifications• Calcein AM/PI for vitality• Support• Contact us• Nuclear staining dyes for live and dead cell identification• GFP reporter• Trypan Blue viability• Detection of apoptotic caspase 3 protein

For research use only.
Not for use in diagnostic procedures.
8 |Cellometer Ascend automated cell counter

SOFTWARE• Introduction• Features and benefits• Technology• Reagents and consumables• Software• Performance• Specifications• Support• Contact usAnalysis elevated.
Matrix, Revvity’s cell counting analysis software, now teamed-up with the Cellometer Ascend, is a win-win for users.
Complete with its pre-loaded templates, Matrix simplifies your workflow and accelerates data acquisition, for assays such as viability,apoptosis, and transduction efficiency. The software is comprehensive with built-in templates, and has over 400 pre-loaded cell types.
Additionally, laboratories will benefit from Matrix’s
library of predefined reports as well as the flexibility
to create custom reports with easy-to-generategraphs, images, charts, and tables. As a unique timesaver, a handy ‘Favorites’ feature tags the most-usedassays and cell count settings for speedy setup andexecution in the future.
Cellometer Ascend with Matrix software is
21 CFR Part 11 ready:• Multiple access levels• Password and user protection• Data security• Comprehensive audit trail

For research use only.
Not for use in diagnostic procedures.
9 |Cellometer Ascend automated cell counter

PERFORMANCE• Introduction• Features and benefits• Technology• Reagents and consumables• Software• Performance• Specifications• Support• Contact us

Confidence elevated.
The versatile Cellometer Ascend can analyze various
cell sample types - both primary cell samples as wellas cultured cells, at a wide range of concentrations. In
particular, the system has been optimized to analyze low
concentration samples for applications such as single
cell sequencing.

Cellometer Ascend concentration range

R2 = 0.9954

As well as measuring viable cell counts andconcentrations, the instrument can also perform a rangeof common cell-based assays including apoptosis, nuclei
counting and GFP reporter gene expression.

Graph of results for cell concentration dynamic range on the Cellometer
Ascend. Data set was taken on a concentration series of cultured Jurkat cellline. Samples from 2 x 104 – 4 x 107 cells/mL can be counted without furtherdilution. The %CV at each concentration was below 10%.

For research use only.
Not for use in diagnostic procedures.
10 |Cellometer Ascend automated cell counter• Introduction• Features and benefits• Technology• Reagents and consumables• Software• Performance• Specifications• Support• Contact us

Specifications
Includes:Instrument specifications:

Imaging performance:• Cellometer Ascend automated cellcounter with integrated touchscreen
computer and dual-fluorescence
optic channels

Weight:24 lbs (10.5 kg)

Cell size:

5 – 80 microns

Dimensions:

Width: 9.9” (25 cm)
Depth: 13” (32.8 cm)
Height: 18.7” (47.5 cm)

Conc. range:

2x104 – 4x107 cells/ml• Matrix software• Two ports in front of instrument(one USB A and one USB C)

Input topower adapter:• Three ports in back of instrument(two USB A and one Ethernet)

Output toinstrument:• Power supply and power cord

Display:• Phone/online applications support
during set-up

100-240 VAC,
50/60 Hz, 1.5A
12 VDC, 10A
Touchscreen (10.1”)
with advanced touchcapabilities

Brightfield imaging, fluorescent imaging, andpattern-recognition software to quickly andaccurately decluster, identify, and countindividual cells.
Fluorescence optics:
Excitation/emission:

470 nm/534 nm

Example Fluorophores:• Acridine Orange (AO)
• Calcein AM• CFDA• GFP (Green Fluorescent Protein)

Excitation/emission:

531 nm/655 nm

Example Fluorophores:• Propidium Iodide (PI)• Ethidium Bromide (EtBr)

For research use only.
Not for use in diagnostic procedures.
11 |Cellometer Ascend automated cell counter• Introduction

Count on our support.

• Features and benefits

Revvity focuses on providing laboratory solutions and support to meet andexceed today’s researcher’s specific needs. We collaborate with customers
every day, analyzing new cell types, analyzing new cell-based assays, andexploring new therapeutic areas and methodologies.

• Technology• Reagents and consumables• Software

Through joint application development, we learn from our customers and assist
with integration into their current workflows. Our specialist are committed to
globally supporting cell quantification, in-depth analysis, and optimizing cellbased assays in academic and government research institutions, biotechnology,industrial settings, and more.

• Performance• Specifications• Support• Contact us

Instrument support services

Education services

Maintaining labs is never easy, especially when an instrument is down.
We know you’re responsible for the performance of your laboratory,
and we make sure nothing holds you back. Our field service engineers,
manufacturing site technical services, and research and development
teams are here at your disposal to ensure maximum uptime.

Whether you are looking for a basic instrument refresher
course, simple troubleshooting techniques, generalapplication support, or method optimization, our field
application scientists or service engineers will comedirectly to your lab. Through education, you will gain
knowledge and insights into the latest techniques, not only
increasing your confidence, but also unlocking the fullpotential of your instrument.

You need instruments to be reliable and running with minimal downtime,and you want flexible service agreements that are easy to comprehend.
We understand, and we’re here to help.

For research use only.
Not for use in diagnostic procedures.
12 |www.revvity.com

Revvity, Inc.
940 Winter Street
Waltham, MA 02451 USA
www.revvity.com

For a complete listing of our global offices, visit www.revvity.com
Copyright ©2024, Revvity, Inc. All rights reserved.
1353388