37th Annual Meeting of the Society for Actinomycetes Japan
Dates: September 7 (Thu) - 8 (Fri), 2023
Venue: Higashi Hiroshima Arts & Culture Hall Kurara
September 7 (Thu)
9:00

Venue Opened

9:30

Opening Remark

9:35

Oral Presentation
9:35

O-1

Proposal of a novel species of the genus Nocardia isolated from clinicalspecimens○ Moriyuki Hamada1,2, Narumi Enomoto1, Masahiro Toyokawa3, Hiroki
Takahashi2, Takashi Yaguchi2(NITE, NBRC1, Mycology Res. Cent., Chiba Univ.2, Fukushima Med. Univ.3)

9:48

O-2

Extensive screening of bioactive compounds in Indonesian Actinomycetes○Rukman Muslimin1,2, Alimuddin Ali3, Nurjannah3, Sho Ogaki1, Kuninobu
Inada4, Hiroki Koyama5, Hisato Kuniyoshi1, Kenji Arakawa1(1Grad. Sch. Integr. Sci. Life, Hiroshima Univ., 2STIFA Makassar, 3Biol.
Dept., Makassar State Univ., 4N-BARD, Hiroshima Univ., 5Grad. Sch. Mar.
Sci. Tech., Tokyo Univ. Mar. Sci. Tech.)

10:01 O-3

Various properties and anticancer activities of actinomycetes isolated
from Izu-Akazawa deep-sea water○ Chiaki Imada1, Jun Wakasugi1 , Yuji Shibata2 , Katsuhisa Yamada2(1Tokyo Univ. of Mar. Sci. and Technol., 2DHC Co.)

10:14

Break（10 min）

10:24

Short Presentation by Conference Sponsors

10:36

Oral Presentation
10:36 O-4

Secondary metabolic enhancement by environmental stress
Kento Tsuchiya1，Yuki Hasuike1，Ryusuke Usuda1, ○Masakazu Kataoka1,
2, 3, 4

(1Grad, Sch. BioMedical Sc. Shinshu Univ., 2RCAM Shinshu Univ., 3 CREST・JST, 4COI-NEXT・JST)
10:49 O-5

A possible mechanism of erythromycin resistance in Streptomyces 23S
rRNA mutants○Kosuke Tanioka1, Yuta Nakashima1, Yu Imai2, Takeshi Hosaka1,2（1Grad. Sch. of Sci. and Technol., Shinshu Univ., 2IBS-ICCER, Shinshu
Univ.）

11:02 O-6

Microbial

chemicalcommunications

drive

hyphalbranchingofactinomycetes○Shumpei Asamizu1,2, Manami Kato1,3, Hiroyasu Onaka1,4(1Univ. of Tokyo., 2Kobe Univ., 3Keio Univ., 4Gakushuin Univ.)
11:15

Break（10 min）

11:25

Invited Lecture I
How c-di-GMP controls progression through the Streptomyces life cycle
Mark Buttner（John Innes Centre, UK）

12:00

Break（90 min, Lunch）

13:30

The SAJ Plenary Meeting

14:00

Award Ceremony

14:25

Award Lecture (Ōmura Award)
Biosynthetic studies on bioactive secondary metabolites in actinomycetes
Ikuro Abe (Graduate School of Pharmaceutical Sciences, The Univesity of Tokyo)

15:05

Award Lectures (Hamada Award)
Biosynthetic studies on the nucleoside antibiotics produced by actinobacteria
Taro Shiraishi（Graduate School of Agricultural and Life Science, The University ofTokyo）

15:25

Break（15min）

15:40

Invited Lecture II
Actinobacterial Response to Oxidants and Antibiotics: Novel Strategies
Jung-Hye Roe (Seoul National University, Korea)

16:15

Break（15min）

16:25

Poster Session I（odd numbers）

17:25

Break

17:30

Move to the banquet venue

18:30

Banquet（18:30 ‒ 20:30）

20:30

Banquet close

September 8 (Fri)
09:00

Venue Opened

09:15

Oral Presentation
9:15

O-7

Involvement

of

the

sortase-mediatedcellwall-localized

protein

AMIS_68180 in sporangium formation in Actinoplanes missouriensis○Zhuwen Tan1, Takeaki Tezuka1,2, Yasuo Ohnishi1,3(1Grad. Sch. of Agric. and Life Sci., The Univ. of Tokyo, 2Grad. Sch. Infection
Control Sci., Kitasato Univ., 3CRIIM, The Univ. of Tokyo)
9:28

O-8

Taxonogenomic analysis of marine-derived Streptomyces sp. N11-50 andthe profile of PKS and NRPS gene clusters○Hisayuki Komaki1, Yasuhiro Igarashi2, Tomohiko Tamura2(1NBRC, 2Fac. Eng., Toyama Prefectural Univ.)

9:41

O-9

Isolation, structure determination, and analysis for thermotolerancepromoting mechanism of heat shock metabolite (HSM) produced by

Streptomyces sp. JA74
○ Yurika Okumura1, Shun Saito1, Kayo Funayama1, Keisuke Fukaya2,
Daisuke Urabe2, Midori A. Arai1(1Grad. Sch. Sci. Tech., Keio Univ., 2Grad. Eng. Biotec., Toyama Pref. Univ.)
9:54

O-10

Biosynthetic Studies on Antitumor Antibiotic Mitomycins○ Yudai Takahashi1, Yo Nakagawa1, Takeshi Tsunoda2, Tohru Dairi2,
Yasushi Ogasawara2(1Grad. Sch. Chem. Sci. Eng., Hokkaido Univ., 2Grad. Sch. Eng., Hokkaido
Univ.)

10:07 O-11

Heterologous production of a new lanthipeptide nocardiopeptin○Ryo Kobayashi, Keita Saito, Shinya Kodani(Grad. Sch. Agr. Sci. Shizuoka Univ.)

10:20 O-12

Study for fluostatins, Actinomycete secondary metabolites○ Shigehiro Tohyama, Masaki Hatano, Chigusa Hayashi, Hideyuki
Muramatsu, Isao Momose, Hiroyuki Igarashi(Institute of Microbial Chemistry)

10:33 O-13

Structural and mechanistic insights into the ATP-dependent isomerization
during biosynthesis of albomycins○ Richiro Ushimaru1,2, Takahiro Mori1, Ziyang Zheng2, Hung-wen Liu2,
Ikuro Abe1
(1Grad. Sch. Pharm. Sci., Univ. Tokyo., 2Dept. Chem., UT at Austin)

10:46

Break（10min）

10:56

Poster Session II（even numbers）

11:56

Break（74 min, Lunch）

13:10Invited Lecture III
Structural enzymology and diversity-oriented biosynthesis for development of new
glycopeptide antibiotics
Tsung-Lin Li (Academia Sinica, Taiwan)

13:45

Break（10min）

13:55

Oral Presentation
13:55 O-14

Biosynthesisofverticilactams

produced

Streptomycesbyspiroverticillatus JC-8444○ Yu Zheng, Katsuyuki Sakai, Hiroshi Takagi, Yumi Shiozaki-Sato,
Toshihiko Nogawa, Shunji Takahashi(RIKEN CSRS)
14:08 O-15

Structural and functional analysis of the ATP-dependent diazotase CmaA6○Seiji Kawai1, Yohei Katsuyama1,2, Yasuo Ohnishi1,2(1Grad. Sch. of Agric. and Life Sci., The Univ. of Tokyo, 2CRIIM, The Univ.
of Tokyo)

14:21 O-16

Improvement of the cellular internalization of the canonical CPP-protein
conjugates by ε-poly-L-α-lysine○ Yamato Takeuchi, Fumihito Hasebe, Chitose Maruyama, Yoshimitsu
Hamano(Biosci. Biotec., Fukui Pref. Univ.)

14:34 O-17

Thymoqinone is an organocatalyst causing oxidative stress
Takahide Miyamoto1, ○Tatsuya Nishiyama2, Takehiro Nagasawa2, Kenji
Ueda1,2(1Grad. Sch. Biosci. Biotec, Nihon Univ., 2College of Bioresource Sciences,
Nihon Univ.)

14:47 O-18

Extracellular expression of Streptomyces spp. laccases in Rhodococcus

erythropolis○Jyothi Priya Putcha1,2 , Wataru Kitagawa1,2(1Grad School of Agriculture, Hokkaido University,

2

Bioproduction

Research Institute, AIST)
15:00 O-19

Surface structure of cells that constitute floating flocs specifically formed
during hexadecane degradation by Rhodococcus jostii RHA1
○Kohei Inoue1, Noraiza Suhaim1, Nurul Syahirah Shamsol Anuar1, Keisuke
Miyazawa2, Takeshi Fukuma2, Yosuke Tashiro3, Kenshi Suzuki1, Naoki
Sunagawa1, Yasuo Ohnishi1,4, Hirofumi Hara1,4(1Grad. Sch. of Agric. and Life Sci., Univ. of Tokyo, 2Inst. of Sci. and Eng.,
Kanazawa Univ., 3Grad. Sch. of Intgr. Sci. Technol., Shizuoka Univ.,

4

15:13 O-20

CRIIM, Univ. of Tokyo)

Design of rpoB mutation using machine-learning Sparse analysis.
○ Takashi Tamura1, Hiromu Hasegawa1, Tadayoshi Kanao1, Michiko
Nemoto1, Michio Yamamoto2(1Grad. Sch. Env. Life Sci., Okayama Univ., 2Osaka Univ.)

15:26

Break（5 min）

15:31

Poster Award Ceremony

15:50

SAJ38th Announcement

15:55

Closing Remarks

Poster Presentation
P-1

Taxonomic study of Actinoplanes isolates isolated from Republic of the Union of
Myanmar and Japan○Masaru Watanabe1, Tomohiro Arai1, Tomohiro Iwabuchi1, Moriyuki Hamada2,
Tomohiko Tamura2, Susumu Kokubo1, Youji Nakagawa1, Nyunt Phay3, Masayuki
Hayakawa1,4, Hideki Yamamura1(1Fac. Life Environ. Sci,, Univ. Yamanashi, 2NITE-NBRC, 3Pathein Univ., 4Yamanashi
Pref. Univ.)

P-2

Optimization of the method for isolating actinomycetes from a single soil particle○Mizuki Abe1, Yuka Nakanishi2, Yu Imai3, Takeshi Hosaka1,2,3（1Grad. Sch. of Sci. and Technol., Shinshu Univ. 2Fac. of Agric., Shinshu Univ. 3IBSICCER., Shinshu Univ.）

P-3

Explore for useful actinomycetes with NO signal○Masaki Takei1, Shinsaku Ito2, Shunsuke Yajima2, Yasuyuki Sasaki2(1Grad.Sch Life Sci.,Tokyo Univ.of Agri., 2Grad. Sch.Agri.,Tokyo Univ.of Agri.)

P-4

Isolation of actinomycetes in soil environment using Water-in-Oil Droplets (WODL)○Jo Saito1, Akihiro Nakamura2, Yuu Fujimura3, Wataru Ogasawara2, Natsumi Saito4(1Dept. Adv. Eng., NIT, Tsuruoka College, 2Nagaoka Univ. of Tech., 3 On-Chip
Biotechnologies Co., Ltd, 4Dept. Creative Eng., NIT Tsuruoka College)

P-5

Taxonomic identification and secondary metabolite evaluation of Krasilnikovia related
strains isolated from Republic of the Union of Myanmar and Japan○Shunsuke Waki1, Yuki Higashiyama1, Maho Inoue1, Moriyuki Hamada2, Tomohiko
Tamura2, Susumu Kokubo1, Youji Nakagawa1, Nyunt Phay3, Masayuki Hayakawa1,4,
Hideki Yamamura1(1Fac. Life Environ. Sci, Univ. Yamanashi, 2NITE-NBRC, 3Pathein Univ., Yamanashi
Pref. Univ.)

P-6

Isolation and taxonomic study of actinomycetes from agricultural products andfertilizers○Akira Také1, Yoshihiko Sakaguchi2, Yuki Inahashi3,4, Kazuyoshi Gotoh5, Shunji
Hayashi1(1Sch. Med., Kitasato Univ., 2Pharm. Sci., Tokushima Bunri Univ., 3Grad. Sch. Infection
Control Sci., Kitasato Univ., 4Ōmura Satoshi Mem. Inst., Kitasato Univ., 5Grad. Sch.
Health Sci., Okayama Univ.)

P-7

Verification of effectiveness for lysozyme pretreatment in selective isolation ofactinomycetes○Hanako Naito, Tomomi Asano, Narumi Enomoto, Tomohiko Tamura, Moriyuki
Hamada(NITE・NBRC)
P-8

Genome analysis of rare actinomycete, Kitasatospora cystarginea NBRC14836○Junfu Morii, Daichi Morita, Teruo Kuroda, Takanori Kumagai(Grad. Sch. Biomed. Health Sci., Hiroshima Univ.)

P-9

A rare-cutting restriction endonuclease-mediated genome editing system for
Actinobacterial strains○Syunsuke Kabaya1, Nobuki Sakurai2, Tadao Oikawa1, Kazuya Yamanaka1(1Grad. Sch. Sci. Eng. Kansai Univ., 2JNC Corp. Yokohama RC)

P-10

Engineering a diaminopimelate pathway in Streptomyces albulus for enhanced
production of ε-poly-L-lysine○Yoshiya Miyake1, Fumihito Hasebe2, Yoshimitsu Hamano2, Tadao Oikawa1, Kazuya
Yamanaka1(1Grad. Sch. Sci. Eng. Kansai Univ., 2Fukui Prefectural Univ.)

P-11

Observation of the unique cellulargrowth of the genus Sporichthya○Xinzhe Guo1, Norio Takeshita2, Takeaki Tezuka1,3, Yasuo Ohnishi1,4(1Grad. Sch. of Agric. and Life Sci., Univ. of Tokyo, 2MiCS, Faculty of Life and Environ.
Sci., Univ. of Tsukuba, 3Grad. Sch. of Infection Control Sci., Kitasato Univ., 4CRIIM,
Univ. of Tokyo)

P-12

Identification of AMIS_66880-66890 as key genes for sporangium membrane
formation in Actinoplanes missouriensis○Hayato Ito1, Takeaki Tezuka1,2, Yasuo Ohnishi1,3(1Grad. Sch. of Agri. and Life Sci., Univ. of Tokyo, 2Grad. Sch. of Infection Control Sci.,
Kitasato Univ., 3CRIIM, Univ. of Tokyo)

P-13

Search for a lipase(s) involved in sporangium dehiscence in Actinoplanes

missouriensis○Shixuan Hu1, Takeaki Tezuka1,2, Yasuo Ohnishi1,3(1Grad. Sch. of Agric. and Life Sci., Univ. of Tokyo, 2Grad. Sch. of Infection Control Sci.,
Kitasato Univ. 3CRIIM, Univ. of Tokyo)
P-14

Light-inducible and genome-integrative high expression system "iLiEX"○Airi Watanabe, Kibune Chihiro, Hideaki Takano(Grad. Sch. Bioresour. Sci, Nihon Univ.)

P-15

High production system of recombinant protein in Streptomyces using genomeintegrative vector○Taichi Okazaki, Hideaki Takano(Grad. Sch. Bioresour. Sci, Nihon Univ.)

P-16

Establishment of a simple DNA transfer system for Rhodococcus spp. and its
application for light-inducible gene expression○Soichiro Mitsuke, Hideaki Takano

(Grad. Sch. Bioresour. Sci, Nihon Univ.)
P-17

Development and application of a novel vitamin B12 bioassay using Actinomycetes○Zhang Yushu, Hideaki Takano(Grad. Sch. Bioresour. Sci, Nihon Univ.)

P-18

Multicopy number plasmid-based light-inducible expression system "pLiEX" inStreptomyces○Ryuta Noya, Hideaki Takano(Grad. Sch. Bioresour. Sci, Nihon Univ.)
P-19

Analysis of the concentration-dependent effects of antibiotics on spore germination ofactinomycetes○Keiichiro Mukai1,3, Ami Yoshimatsu2, Yu Imai3, Takeshi Hosaka1,2,3（1Grad. Sch. of Med. Sci. and Technol., Shinshu Univ., 2Fac. of Agric., Shinshu Univ.,
3

P-20

IBS-ICCER, Shinshu Univ.）

Analysis of the concentration-dependent effects of ribosome-targeting antibiotics on
streptomycetes○Tomoko Shibayama1, Keiichiro Mukai1,2, Yu Imai2, Takeshi Hosaka1,2(1Grad. Sch. of Sci. and Technol., Shinshu Univ., 2IBS-ICCER, Shinshu Univ.

P-21

Effects of acid stress on nucleic acid antibiotic production and expression of quality
control genes in the actinomycete Streptomyces incarnatus○Mao Kubo, Haruka Yamagata, Yuriko Nakashima, Tadayoshi Kanao, Michiko
Nemoto, Takashi Tamura(Grad. Sch. Env. Life. Sci, Okayama Univ.)

P-22

The Effect of mutagenesis of the rpoB gene on H457 to increase sinefungin
production○Hiromu Hasegawa, Tadayoshi Kanao, Michiko Nemoto, Takashi Tamura(Grad. Sch. Env. Life Sci., Okayama Univ.)

P-23

Novel contractile injection systems of Streptomyces davawensis JCM4913○Toshiki Nagakubo1,2, Tatsuya Nishiyama3, Tatsuya Yamamoto1, Nobuhiko Nomura1,2,4,
Masanori Toyofuku1,2(1Life and Environ. Sci., Univ. Tsukuba, 2MiCS, Univ. Tsukuba, 3Biores. Sci., Nihon
Univ., 4TARA Center, Univ. Tsukuba)

P-24

Isolation, structure determination and analysis for production mechanism of heatshock metabolites (HSMs) produced by Streptomyces sp. AY2○Yanagi Mori1, Shun Saito1, Yohei Kathuyama2,3 , Yasuo Ohnishi2,3, Midori A. Arai1(1Grad. Sch. Sci. Tech., Keio Univ., 2Grad. Sch. of Agric. and Life Sci., and 3CRIIM,
UTokyo)P-25

Isolation, structure determination, and evaluation of thermotolerance-promoting
activity of heat shock metabolites (HSMs) produced by Streptomyces sp. HR41

○Shun Saito, Sosuke Kataoka, Shiina Suzuki, Midori A. Arai
(Grad. Sch. Sci. Tech., Keio Univ.)
P-26

Functional analysis of the keto reductase Gra-6 regulates the stereochemistry of
granaticin biosynthesis (2nd)○Kazuki Ishikawa1,2, Noriko Kusuoku1, Minori Hashimoto1, Chiharu Nozaki1, Makoto
Hashimoto1,2, Koji Ichinose1,2(1Fac. Pharm. Musashino Univ., 2Research Institute of Pharm. Sci., Musashino Univ.)

P-27

Sulfoxidation of labionin enhances antibacterial activity○Shinta Ijichi, Shotaro Hoshino, Shumpei Asamizu, Hiroyasu Onaka(Dept. of Life Sci., Gakushuin Univ.)

P-28

Exploration of arsenic secondary metabolic pathways distributed in actinomycetes○Shotaro Hoshino, Shumpei Asamizu, Hiroyasu Onaka(Dept. of Life Sci., Gakushuin Univ.)

P-29

New salicyloylglycerol from Thailand coral-derived Micromonospora○Haruka Doyo1, Enjuro Harunari1, Wongsakorn Phongsopitanun2, Somboon
Tanasupawat2, Makamas Sutthacheep3, Thamasak Yeemin3, Yasuhiro Igarashi1（1Toyama Pref. Univ., 2Chulalongkorn Univ., 3Ramkhamhaeng Univ.）

P-30

Novel phenylpropanoid produced by protoplastization of Streptomyces○Enjuro Harunari, Nodoka Yago, Yasuhiro Igarashi（Toyama Pref. Univ.）

P-31

Study on the biosynthetic mechanism of β-homolysine found in resormycin○Chisaki Imahori1, Kazuya Yamanaka2, Yasushi Ogasawara3, Masayuki Igarashi4,
Tohru Dairi3, Fumihito Hasebe1, Yoshimitsu Hamano1, Chitose Maruyama1(1Grad. Sch. Biosci. Biotec., Fukui Pref. Univ., 2Fac. Chem. Mater. Bioeng., Kansai Univ.,3

P-32

Grad. Sch. Eng., Hokkaido Univ., 4Institute of Microbial Chemistry, BIKAKEN.)

Identification of novel methionine biosynthetic pathway in Streptomyces albulus○Kazuya Adachi, Chitose Maruyama, Yoshimitsu Hamano, Fumihito Hasebe(Grad. Sch. Biosci. Biotec., Fukui Pref. Univ.)

P-33

Searching for Specific Inhibitors of the Alternative Pathway of Peptidoglycan
Biosynthesis
Shuhei Umetsu1, Takeshi Tsunoda2, Yuki Inahashi3, Kenichi Nonaka3, ○Yasushi
Ogasawara2, Tohru Dairi2(1Grad. Sch. Chem. Sci. Eng and 2Grad. Sch. Eng, Hokkaido University, 3Ōmura Inst.,
Kitasato Univ.)

P-34

Biosynthetic studies on natural product containing ⍺,⍺-disubstituted amino acid
○Takeshi Tsunoda1, Shunkichi Furumura2, Haruka Yamazaki2, Yasushi Ogasawara1,
Tohru Dairi1(1Grad. Sch. Eng., Hokkaido Univ., 2Grad. Sch. Chem. Sci. Eng., Hokkaido Univ.)

P-35

Direct cloning and refactoring of an orphan biosynthesis gene cluster putatively for
non-ribosomal peptide with branching structure in Streptomyces lydicamycinicus○Daichi Kitaura, Tadao Oikawa, Kazuya Yamanaka(Grad. Sch. Sci. Eng. Kansai Univ.)

P-36

Isolation of new kinanthraquinones and analsis of biosynthetic mechanism using aheterologous expression system○Katsuyuki Sakai, Yushi Futamura, Hiroyuki Koshino, Hiroyuki Osada, Shunji
Takahashi(REKEN CSRS)

P-37

Expression of SYO_1.56 SARP Regulator Unveils Unprecedented Elasnin Derivatives
with Remarkable Antibacterial Activity○Islam A. Abdelhakim1,2, Yushi Futamura3, Naoko Kito1, Arisa Shibata4, Sachiko
Masuda4, Ken Shirasu4, Hiroyuki Osada3, Jun Ishikawa5, Shunji Takahashi1(1Nat. Prod. Biosynth., RIKEN CSRS, 2Fac. Pharm., Assiut Univ., 3Chem. Res. Dev.,
RIKEN CSRS, 4Plant Immunity, RIKEN CSRS, 5NIID)

P-38

The Structure-Activity Relationship of Novel Antibiotic MM249 Compounds○Makiko Komagata1,2, Hikaru Abe1, Maya Umekita1, Atsushi Tsugita3, Yoshimasa
Ishizaki1, Rie Arisaka1, Masaki Hatano1, Tomoyuki Kimura1, Ryuichi Sawa1, Yoshikazu
Tanaka3, Takeshi Yokoyama3, Takumi Watanabe1, Masayuki Igarashi1(1Institute of Microbial Chemistry, BIKAKEN, 2Grad. Sch. Sci. & Eng., Keio Univ., 3Grad.
Sch. Life Sci., Tohoku Univ.)

P-39

Crystal structure analysis of cytochrome P450 enzyme RosC catalyzing the same-site
multistep oxidation reactions○Yohei Iizaka, Hironori Suzuki, Haruki Kawasaki, Kanon Fujimoto, Atsushi Fukumoto,Shuji Noguchi, Yojiro Anzai(Fac. Pharmaceutical Sci. Toho Univ.)

P-40

Study of quorum sensing inhibitors with α-pyridone ring structure produced byactinomycetes○Jiahao Zeng, Asumi Kasahara, Riku Takeuchi, Yohei Iizaka, Atsushi Fukumoto, Yojiro
Anzai(Fac. Pharm. Sci., Toho Univ.)

P-41

Isolation and proposed biosynthetic pathway of a novel secondary metabolite
produced by amino group-carrier protein in Streptomyces lydicus ATCC 25470○Yuta Hayashi1, Yusuke Sone1, Ayako Yoshida1,2, Saori Kosono1,2, Makoto
Nishiyama1,2(1Grad. Sch. of Agri. and Life Sci., UTokyo, 2CRIIM, UTokyo)

P-42

Modification of the substrate recognition of AzpC, an AMPylation enzyme derived
from alazopeptin biosynthesis

○Takashi Maruyama1, Kouki Miyake1, Seiji Kawai1, Yoshitaka Moriwaki1,2, Tohru
Terada1,2, Yohei Katsuyama1,2, Yasuo Ohnishi1,2（1Grad. Sch. of Agric. and Life Sci., The Univ. of Tokyo, 2CRIIM, The Univ. of Tokyo）
P-43

The biosynthesis of streptazones, polyketides with a cyclopenta[b]pyridine ring from

Streptomyces○Taichi Hiramatsu1, Yohei Katsuyama1, 2, Yasuo Ohnishi1, 2(1Grad. Sch. of Agric. and Life Sci., The Univ. of Tokyo, 2CRIIM, The Univ. of Tokyo)
P-44

Elucidation of the pathway leading to spirocycle formation in yoropyrazone
biosynthesis○Suzuna Kiyofuji1, Yohei Katsuyama1,2, Yasuo Ohnishi1,2(1Grad. Sch. of Agric. and Life Sci., The Univ. of Tokyo, 2CRIIM, The Univ. of Tokyo)

P-45

Characterization of a tyrosine isocyanide synthase HzmA
〇Hiroto Maruyama, Kumiko Imachi, Kenichi Matsuda, Toshiyuki Wakimoto(Faculty of Pharmaceutical Sciences, Hokkaido University)

P-46

Metabolomic analysis of membrane-vesicles-induced natural product fromactinomycetes○Ryusuke Nakada, Aya Yoshimura, Toshiyuki Wakimoto(Faculty of Pharmaceutical Science, Hokkaido University)

P-47

Identification of lavencidin biosynthetic genes in S. lavendulae FRI-5 by Target-AID
technology○Ryo Otsuka1, Yu Sato2, Eiji Okamura3, Kohsuke Honda1,4, Shigeru Kitani1,3（1ICBiotech, Osaka Univ., 2Grad. Sch. Sci. Tech. Innov. Agric., Yamaguchi Univ., 3Col.
Sci. Eng., Aoyama Gakuin Univ., 4OTRI, Osaka Univ.）

P-48

The metabolism of black pepper bioactive compound in an actinomycete○Pu Jian1, Takuto Kumano2, Mio Kimura3, Makoto Kurisaki3, Yoshiteru Hashimoto2,
Michihiko Kobayashi2(1Agro-biological resource sciences, University of Tsukuba, 2MiCS, University ofTsukuba, 3Graduate School of Life and Environmental Sciences, University ofTsukuba)

P-49

Genome analysis of rare actinomycete Actinoplanes nipponensis NBRC14063○Takuma Iwami, Daichi Morita, Teruo Kuroda, Takanori Kumagai(Grad. Sch. Biomed. Health Sci., Hiroshima Univ.)

P-50

Analysis of molecular diversity and versatility of butenolide-type signaling molecule
SRB○Asahi Hirata1, Momoko Akimoto1, Hazuki Fujita2, Miho Sumiyoshi3, Teshima Aiko1,
Kenji Arakawa1,2（1Grad. Sch. Integr. Sci. Life, Hiroshima Univ., 2Hiroshima Univ. 3Grad. Sch. AdSM,
Hiroshima Univ.）

P-51

Structural and biosynthetic investigation of novel hydrazide compounds produced in amutant KA57 of Streptomyces rochei○Haruka Nagano1, Yu Tanaka1, Mei Okano1, Takuya Kishimoto2, Ayaka Tatsukawa2
Hirofumi Kunitake2, Kaito Fukumori3, Atsushi Fukumoto4, Yojiro Anzai4, Kenji
Arakawa1,2,3(1Grad. Sch. Integr. Sci. Life, Hiroshima Univ., 2Grad. Sch. AdSM, Hiroshima
Univ., 3Hiroshima Univ., 4Faculty of Pharmacy, Toho Univ.)

P-52

Functional evaluation of esterified lankacidin derivatives based on computational
prediction〇Mei Okano1, Natsumi Nishiura2, Rukman Muslimin2, Aiko Teshima2, Mohamed Ali
Elrefaiy3, Kiep Min Do4, Takeshi Kodama4, Hiroyuki Morita4, Ahmed T. Ayoub5, Kenji
Arakawa1,2（1Grad. Sch. Integr. Sci. Life, Hiroshima Univ., 2Grad. Sch. AdSM, Hiroshima Univ.,3

P-53

Zewail City of Science and Technology, 4Toyama Univ., 5HToO Bioscience）

Approach to genome mining for secondary metabolites using the Streptomyces
butenolide-type signaling molecules○Momoko Akimoto1, Asahi Hirata1 , Miho Sumiyoshi2, Toshihiro Suzuki3, Aiko
Teshima1, Kenji Arakawa1(1Grad. Sch. Integr. Sci. Life, Hiroshima Univ., 2Grad. Sch. AdSM, Hiroshima Univ,
3

P-54

Ferment. Sci. Tech. Tokyo Univ. Agric.)

A lankamycin derivative with a branched-chain sugar isolated from a blocked mutant
of chalcose biosynthesis in Streptomyces rochei 7434AN4〇Mingge Zhang1, Bao Shuang2, Kenji Arakawa1,2(1Grad. Sch. Integr. Sci. Life, Hiroshima Univ., 2Grad. Sch. AdSM, Hiroshima Univ.)

P-55

Analysis of the SARP-type transcriptional activators for secondary metabolite
production in actinomycetes〇Yang Liu, Yuya Misaki, Yosi Nindita, Kenji Arakawa(Grad. Sch. Integr. Sci. Life, Hiroshima Univ.)

P-56

Relationship of actinomycetes-derived secondary metabolite in kusaya gravy, a highsalt fermented fish gravy○Sachiko Masaki1, Sakura Nogimura1, Asahi Hirata2, Takahiro Osada3, Kenji
Arakawa2 , Ryosuke Unno1, Morio Ishikawa1, Toshihiro Suzuki1(1Dept. Ferment. Sci., Tokyo Univ. Agric., 2Integ. Sci. life., Hiroshima Univ., 3Osada
Shouten)

P-57

Salt-dependent co-production of secondary metabolites in Streptomyces diastaticus
strain TUA-NKU25 isolated from a Japanese traditional fermented fish product○Miu Ueki1, Aoi Takeuchi1, Asahi Hirata2, Aiko Teshima2, Takuma Ikegami1, Kenichi
Matsuda3, Toshiyuki Wakimoto3, Kenji Arakawa2 , Ryosuke Unno1, Morio Ishikawa1,

Toshihiro Suzuki1(1Dept. Ferment. Sci., Tokyo Univ. Agric., 2Integ. Sci. life., Hiroshima Univ., 3Grad. Sch.
Pharm., Hokkaido Univ.)
P-58

N-Formimidoylation/-iminoacetylation modification in aminoglycosides requires FADdependent and ligand-protein NOS bridge dual chemistryYung-Lin Wang1, Chin-Yuan Chang2, Ning-Shian Hsu1, I-Wen Lo1, Kuan-Hung Lin1,
Chun-Liang Chen1, Chi-Fon Chang1, Zhe-Chong Wang1, Yasushi Ogasawara3, Tohru
Dairi3, Chitose Maruyama4,5, Yoshimitsu Hamano4,5*, Tsung-Lin Li1,6*(1Genomics Res. Ctr., Academia Sinica, 2Dept. Biol. Sci. Technol., National Yang Ming
Chiao Tung Univ., 3Grad. Sch. Engineering, Hokkaido Univ., 4Grad. Sch. Biosci. Biotec.,
Fukui Pref. Univ., 5FBIC, Fukui Pref. Univ., 6Biotec. Ctr., National Chung Hsing Univ.)

P-59

Kasugamycin Analogs: Harnessing Immune Reprogramming and Infiltration in the
Immunosuppressive Tumor Microenvironment by Targeting Chitinases for Potential
Cancer Therapy○An Ning Cheng, Tsung-Lin Li(Genomics Research Center, Academia Sinica, Taipei, Taiwan)

P-60

Biosynthetic studies of glycopeptide antibiotic apovarcin and re-programme pathways
to develop new antibiotics○Kuan-Hung, Lin, Tsung-Lin, Li(Academia Sinica, Genomic Research Center, Taiwan)

P-61

Characterization of metal ion dependent xylose isomerase from Lactococcus subsp.

Lactis○Ning-Shian Hsu, Yung-Ling Wang, Kuan-Hung Lin, Tsung-Lin Li(Genomic Research Center, Academia Sinica)
P-62

Identify the essential cis-acting region for conjugation of SAP1 linear plasmid○Takumi Kato1, Kento Tsuchiya1, Yuta Fukada2, Masakazu Kataoka1(1Dep. Biomed Eng., Grad. Sch. Sci. tech., Shinshu Univ., 2Dep. Bioeng., Grad Sch.
Med. Sci tech., Shinshu Univ)

P-63

Synchronization of growth phases improves the chromosomal mobilization frequency
between different Streptomyces species○Kento Yoda1, Akane Mizowaki1, Masakazu Kataoka1(1 Dept. Biomed. Eng. Grad. Sch. Shinshu Univ)

P-64

The mechanism of antibacterial activity of organocatalysts○Takahide Miyamoto1, Tatsuya Nishiyama2, Takehiro Nagasawa2, Kenji Ueda1,2(1Grad. Sch. Biosci. Biotec, Nihon Univ., 2College of Bioresource Sciences, Nihon
Univ.)

P-65

Crystallization and structural characterization of the actinorhodin binding protein○Mirai Hino1, Mana Fukazawa2, Reina Nagayasu1, Tatsuya Nishiyama2, Takeo

Tomita3,4, Makoto Nishiyama3,4, Kenji Ueda1,2)(1Grad. Sch. Biosci. Biotec, Nihon Univ., 2College of Bioresource Sciences, Nihon Univ.,
3

Graduate School of agriculture and life sciences, The Univ. of Tokyo, 4CRIIM, The

Univ. of Tokyo)
P-66

Spectroelectrochemical analysis of the membrane permeation mechanism offluorescently labeled ε-poly-L-lysine at the biomimetic interface
Hiroki Sakae1, Yamato Takeuchi2, ○Saha Otsuka2, Chitose Maruyama2, Yoshimitsu
Hamano2, Yoshio Nishiyama1, Hirohisa Nagatani1(1Kanazawa Univ., 2.Grad. Sch. Biosci. Biotec., Fukui Pref. Univ. )P-67

Exploring the uncharacterized NRPS in the Actinomycetota genomes○Arisa Nishihara1, Takuya Hashimoto2, Moriya Ohkuma1(1JCM, RIKEN-BRC, 2BPRI, AIST)

P-68

Characteristics of phosphate solubilizing actinomycetes isolated from plantrhizosphere
Runa Sagae, Nobuhiro Sasaki, Eiko Takahashi, Shinko Ito, ○Natsumi Saito(Dept. Creative Eng., NIT Tsuruoka College)

P-69

Mechanism of nitric oxide homeostasis by Mycothiol in Streptomyces coelicolor A3(2)M145○Kogo Minori1, Tomoki Yoshizumi1,Yukiko Shibui2, Sota Honma2, Shinsaku Ito1,2,
Shunsuke Yajima1,2, Yasuyuki Sasaki1,2(1Grad. Sch. Sci. Life, Tokyo Univ. of Agri, 2Grad. Sch. Agri., Tokyo Univ. of Agri,)

P-70

Effect of acetophenone on antibiotic production activity in Streptomyces coelicolor
A3(2) M145
○Nanami Nakajima1, Kana Ishii2, Sota Honma1, Shinsaku Ito1.2, Shunsuke Yajima1.2,
Yasuyuki Sasaki1.2(1Grad.Sch.Tokyo Univ. of Agric・Bio, 2Tokyo Univ. of Agric・Bio)

P-71

Construction of an efficient screening system for modified PKS with novel
fluorescence probe○Manami Kato1,2, Shinichi Sato3, Satoshi Yuzawa1,2(1IAB, Keio Univ., 2Grad. Sch. Med. Govern, Keio Univ., 3FRIS, Tohoku. Univ.)

P-72

Antibiotics productivity improvement via ribosome engineering of Amycolatopsis nivea
strain TUA-HKG02Y isolated from a high salt fermented fish gravy○Aoto Saka1, Kana Shinoda1, Takahiro Osada2, Minenosuke Matsutani3, Kenji
Arakawa4, Ryosuke Unno1, Morio Ishikawa1, Toshihiro Suzuki1(1Dept. Ferment. Sci., Tokyo Univ. Agric., 2Osada Shouten, 3NGRC., Tokyo Univ. Agric.,
4

P-73

Integ. Sci. life., Hiroshima Univ.)

Construction of an agricultural bioformulation based on Streptomyces sp. exhibiting
inhibitory activity against Xanthomonas arboricola pv. Pruni○Yuka Okubo1, Susumu Kokubo1, Youji Nakagawa 1, Mitsuyoshi Soya2, Tomoya Izumi2,
Masayuki Hayakawa3, Hideki Yamamura1(1Fac. Life Environ. Sci, Univ. Yamanashi, 2Suzuken Kogyo Co., Ltd, 3Yamanashi Pref.
Univ.)
P-74

Screening of Actinomycetes Isolates for In-vitro and In-vivo Plant Growth Promotion
Activities○Tanaporn Kimkaew1,2, Wisuwat Songnuan3, Bungonsiri Intra1,2(1Dept. Biotec., Fac. Sci., Mahidol Univ., 2Mahidol univ. & Osaka univ. Collab. Res. Cen.
Biosci. & Biotec., 3Dept. Plant Sci., Fac. Sci., Mahidol Univ.)

P-75

Micromonospora sp. Strain MCN027, a Marine-derived Actinomycetes with Anticancer
Potential○Chananan Ngamcharungchit1,2, Bungonsiri Intra1,2(1Dept. Biotec., Fac. Sci., Mahidol Univ., 2Mahidol univ. & Osaka univ. Collab. Res. Cen.
Biosci. & Biotec.)

P-76

Analyzing the ability to produce secondary metabolites of actinomycetes using
genome information○Yuta Kikuchi1, Hayama Tsutsumi1,2, Yuki Inahashi1,2(1Grad. Sch. Infection Cont. Sci., Kitasato Univ., 2Ōmura Inst., Kitasato Univ.）

P-77

Taxonomic study of Longispora sp. K20-0274 isolated from T-PIRC farm○Yuki Goda1, Hiroki Kanto1, Masanobu Kasuga1, Hayama Tsutsumi1, 2, Yohei
Katsuyama3, Yasuo Ohnishi3, Yuki Inahashi1(1Grad. Sch. Infect. Cont. Sci., Kitasato Univ., 2Kitasato Inst. Life Sci., 3Grad. Sch. ofAgric. and Life Sci., Univ. of Tokyo)

P-78

Biosynthetic study on a compound consisting of maleic anhydride and polyketideskeleton○Reona Suzuki1, Tomoyasu Hirose1,3, Akihiro Ishii1, Ayano Komaki2, Yuka Yamakawa2,
Tomoyasu Hirose1,3, Toshiaki Sunazuka1,3, Yuki Inahashi1,3(1Grad. Sch. Infection Cont. Sci., Kitasato Univ., 2Sch. Science. Kitasato Univ., 3Ōmura
Inst. Kitasato Univ.)