LM1099EAULTRON PS-80 Series
Instruction
Instruction Manual
Manual
1. Introduction
Thank you for purchasing the ULTRON PS-80 series packed column for high-performance liquid
chromatography. The PS-80 series of columns are packed with a strong cation -exchange resin consisting of
sulfonated cross-linked styrene-divinylbenzene copolymer. There are 4 types of columns in the PS -80
series:・ PS-80H: designed especially for analysis of organic acids.

・ PS-80N, C, P : designed especially for analysis of sugars and sugar alcohols.
ULTRON PS-80columns, which are manufactured under highly controlled conditions, must pass a seriesof strict tests before being accepted for shipment. To ensure optimal performanc e and durability of thecolumn, please read these instructions carefully before using this column.
2. Specifications
Base material

Sulfonated styrene-divinylbenzene resin

Degree of cross-linkage

8%Particle size

10 mCounter ion

PS-80H: Hydrogen (H+)
PS-80N: Sodium (Na+)
PS-80C: Calcium (Ca2+)
PS-80P: Lead (Pb2+)

column nameDimensions(mm)Max. operatingpressure (MPa)Max. operating
temperature (oC)flow rate range(mL/min)

PS-80H250 x 2.0

4.0

80 (recommended 60)

0.03 ~ 0.06

PS-80H

300 x 8.0

4.080 (recommended 60)

0.5 ~ 1.0

PS-80N

300 x 8.0

4.080 (recommended 60)

0.5 ~ 1.0

PS-80N

500 x 8.0

6.080 (recommended 60)

0.5 ~ 1.0

PS-80C

300 x 8.0

4.090 (recommended 80)

0.5 ~ 1.0

PS-80P

300 x 8.0

4.090 (recommended 80)

0.5 ~ 1.0

3. Shipment solvent

・PS-80H： aqueous solution of perchloric acid (pH 2.1)
・PS-80N, C, P ： pure water.
4. Mobile phase and sample
・Ensure to filter samples and mobile phases using a membrane filter with a mesh size of 0.45 µm or smaller before
using. Failure to filter mobile phases, etc. can lead to blockages of column filters and increases in analytical pressures.
・Ensure to thoroughly degas mobile phases prior to use. Insufficient degassing of mobile phases can lead to theformation of bubbles inside analytical instruments and columns resulting in problems with analyses.
・The columns in this series are used with water or the appropriate aqueous acid mobile phases. The mobile phase may
contain up to 30% acetonitrile.

LM1099EA

5. Precautions for use
・Before installation the column, replace the liquid in the system with the mobile phase to be used.
・Tubing must have flat ends and must bottom out in the column endfitting. Tubing must be connected to the column
correctly to avoid creating a void between the column frit and tubing, which can cause a leak and result in poor
column performance.
・Install the column according to the direction of the arrow.
・Do not remove the column from LC system before the pressure drops zero.
・Flow rate direction of mobile phases is as indicated on the column label.
・Avoid using a column repeatedly near the pressure limit or making abrupt changes in pressure to prevent shortening of
the column life. The degradation of column performance is likely to occur when used at higher temperatures.
・Please flow the mobile phase at an initial flow rate 0.1 mL/min(0.01 mL/min for PS-80H 250 x 2.0) in order to avoid
the danger of sudden pressure increases.
・Metal ion and amino compounds in sample or mobile phase may degrade the column performance. Please use guard
column in order to avoid the above trouble.
・ Do not subject to shock such as dropping, etc.
・ Performance of the column should be carried out in accordance with the enclosed “COLUMNPERFORMANCE REPORT”.
6. Column cleaning and regeneration
When the column performance deteriorates, try cleaning the column using regeneration solution as below at 0.5mL/min(0.03 mL/min for PS-80H 250 x 2.0) at 50℃ for 2 hours.
(Regeneration column performance)column nameRegeneration solution

Temperature (℃)

ProcedurePS-80H

50mM HCl50

at 0.5 mL/min for 2 hours

PS-80N

50mM NaOH50

at 0.5 mL/min for 2 hours

PS-80C

50mM Ca(NO3)2・4H2O50

at 0.5 mL/min for 2 hours

PS-80P

50mM Pb(NO3)250

at 0.5 mL/min for 2 hours

7.Column storage
・Do not allow the column packing material to dry out.
・Close the column with end stop plugs tightly and store at cool and dark place.

・If it will not be used for a long time, flow the mobile phase at a flow rate of 0.5 mL/min (0.03 mL/min for PS-80H
250 x 2.0) for about 1 hour at least once a month.

ULTRON series packed columns are shipped under highly controlled conditions. However, if you should find any
defect, please contact your dealer or Shinwa.
Note that Shinwa does not warrant the product against column life or deterioration caused by the failure to follow theabove instructions.

50-2 Kagekatsu-cho, Fushimi-ku, Kyoto 612-8307 JAPANTEL +81-75-621-2360

URL https://shinwa-cpc.co.jp