Instructions for UseSarpette® M
SARSTEDT No.: 90.3100.xxx / 90.3108.xxx / 90.3112.xxx

Instructions for Use Sarpette® M

Instructions for Use Sarpette® MContents
1.

Product description
1.1
1.2

2

3

4

3
3Commissioning

3

2.1
2.2
2.3
2.4
2.5

3
3
4
4
4

Positioning for work with the pipette
Set volume
Tip selection
Tip ejection
Protective filter

Pipetting procedure

43.1
3.2

55

Normal pipetting procedure
Reverse pipetting procedure		

Maintenance

5

4.1
4.2
4.3

566666
7778

4.4

4.5

Checking the seal
CleaningDisassembly of the volume unit and replacement of the O-ring, models – 1000 µl
4.3.1
Remove the volume unit (lower part)		
4.3.2
Replacing the O-ring and PTFE sleeve
4.3.3
Replacing the O-ringDisassembly of the volume unit and replacement of the O-ring, models 5 and 10 ml
4.4.1
Remove the volume unit (lower part)
4.4.2
Changing the O-ring
Piston change, multi-channel Sarpette® M

5Faults

8

6

Sterilisation

87

Calibration

97.1

9

In-lab calibration

8Warranty

99

Performance data Sarpette® M

109.1
9.2

10
10

102Safety instructions
Description

3Single-channel Sarpette® M
Multi-channel Sarpette® M8 / M12

Ordering information

1.

Product description

1.1

Safety instructions• Read instruction manual carefully before use and keep for future reference.
• Follow the manufacturer's instructions and instructions for use and maintenance.
• Avoid possible overexertion of the hand during prolonged pipetting work and its medical consequences(e.g. tendonitis).

1.2

Description

The Sarpette® M allows precise and effortless pipetting. The single-channel models cover a volume range of
0.1 μl to 10 ml. The six models of the 8- or 12-channel versions in the range of 0.5 to 300 μl are perfectly suited
for a uniform liquid transfer in microtiter plates. All models are fully assembled and autoclavable at 121°C. The
main advantages are the digital display (E) for a permanent volume display and the innovative JustipTM system(H) with a softly padded ejection button (C) for optimum tip ejection. The Swift Set calibration system (F) allows
readjustments by the user.

2Commissioning
AABBCCDDEEFF~ 0.5 mm~ 0.5 mm

HG~ 0.5 mm~ 0.5 mm~ 0.5 mmKHII

Fig. 1

2.1

Fig. 2

Fig. 3

Positioning for work with the pipette

Place the finger rest (D) on the distal phalange of the index finger. The push button (B) and tip ejector (C) canbe actuated by a slight movement of the thumb. The rotatable multi-channel housing (K) enables individual
selection of the best possible adapted working position.

2.2

Set volume

The volume is adjusted by turning the push button (B) until the desired volume appears in the digital display (E)(turning clockwise reduces the volume and vice versa). Sensitive click stops of the micrometre screw and thefreely rotating coloured pushbutton cap (A) prevent unwanted adjustments during ongoing pipetting operations.

113

Instructions for Use Sarpette® M

Instructions for Use Sarpette® M
3.1			When the letter (E) lights up next to the numbers in the display, the selected volume is
no longer in the working range of the pipette. Over-rotating the micrometre screw candamage the mechanism.

2.3

Tip selection

The SARSTEDT Refill Revolution pipette tips provide the best possible combination of pipette and tip for every
application. Use only tips offered by the manufacturer or compatible with them. Further information and adetailed brochure can be found at www.refillrevolution.tips.

The precisely adjusted volume is aspirated and then discharged.
-Phase 1:
Press the operating button until the first stop is reached.
Phase 2: 	Immerse the tip vertically approx. 2 – 3 mm deep and slowly release the button. Wait 2
seconds, remove pipette and filled tip vertically without touching the container.
- Phase 3:		 Place the tip against the wall of the second container, slowly press the operating button to
the first stop to eject liquid.
- Phase 4:		 Press the operating button completely. Residues are expelled from the tip.			 Draw the pipette tip 10-15 mm along the wall of the receiver tube.
- Phase 5:		 Discard the used tip by pressing the ejection button (C).

3.2			

Reproducibility is improved if each tip is flushed with the material to be pipetted
at least once before use.

Normal pipetting procedure (Fig. 4)

Reverse pipetting procedure

Any excess volume is aspirated, but only the set volume is dispensed.

The patented JustipTM system allows immediate positioning of the shaft height (± 2 mm) for an ideal adjustment
and perfect ejection of the tips. The distance between the tip and the ejection rod is set to ~0.5 mm by turning
the ejection screw (< LO – HI >). Built-in click stops prevent unwanted adjustments.

Press the operating button until the overstroke (second stop) is reached.
Immerse the tip vertically approx. 2-3 mm deep and slowly release the button. Wait 2 sec.,
remove the pipette and filled tip vertically without touching the container wall.
- Phase 3:
Place the tip on the wall of the second container and press button only up to the first			 stop, to deliver the exact set amount of liquid.
- Phase 4:		Remove the pipette from the second container and repeat Phase 2. Expel the remaining
content by pressing the button until the second stop (overstroke). Discard the used tip bypressing the ejection button (C).

2.54

2.4

Tip ejection (Figs. 2 and 3)

Protective filter

Macropipettes (5 and 10 ml models) can be provided with a protective filter for additional safety against
penetration of liquid and risk of contamination. This is recommended when dosing large volumes and/or toxic,
radioactive or highly aggressive liquids. It is essential to replace a wet or contaminated filter. The filters are notautoclavable.

3-Phase 1:		Phase 2:		Maintenance

As a rule, the models do not require maintenance. Continuous care and cleaning contribute to the optimalfunction and longevity of the instrument. It is recommended to check the performance data according to internal
control procedures (SOP, GLP, etc.) or at least once a year. Replace defective parts only with original parts fromthe manufacturer.

Pipetting procedure
43

Before pipetting, a new, clean tip must be firmly attached.

2123 41234cc2

31
13Fig. 6~ 0.5 mm

Fig. 8ﬂexion sur la tête du piston.

Forward pipetting

4Fig. 7

Fig. 4

Reverse pipetting

Fig. 5

4.1

Checking the seal

Correct functioning of the instrument is only possible if the air cushion closes tightly. Leakage is shown bydroplet formation at the tip or by a different liquid level in the individual tips of a multichannel pipette. In the eventof a leak, we recommend that you have the pipette serviced.

5

Instructions for Use Sarpette® M
4.2

Cleaning

Instructions for Use Sarpette® M
4.4

Disassembly of the volume unit and replacement of the O-ring, models 5 and 10 ml (Fig. 7)
1

The removed volume unit (lower part) of the pipette can be cleaned with water or alcohol. In cases of heavy
soiling, the individual parts can also be immersed in a disinfectant solution. Proper cleaning is necessary if liquid
has inadvertently penetrated into the pipette interior. Rinse and dry the instrument before assembly. Remove
particularly stubborn residues in the ultrasonic bath. Before assembling the pipette, lightly grease the O-ring (seebelow).

235

4.3

Disassembly of the volume unit and replacement of the O-ring, models – 1000 µl (Fig. 6)

6

4.3.1

Remove the volume unit (lower part):

7

4
12

121212121212 12

13

13

13

21
13
1321 21

22

22 22

1415
23
1515 15
23 23

16
17

17
16
17
13

17 17

18

18
18

1415

1. Press the ejection button (2), turn the ejection nut (20) or (24) to the left for 2 ml and remove.
2. Unscrew the cylinder (18).

83. Push the operating button down completely, then pull out the piston.

918182424 242525 25

1818 182728

13 13

2933

4.3.230

10Replacing the O-ring and PTFE sleeve:

1. After removing the lower part, remove the cylinder (18) and press it onto both mandrels of the ring (14) withthe aid of a pipette tip or a pointed object.

191919

19
19

20

20

2020203. Replace defective parts. Remove excess grease from the piston (13).

To avoid possible damage to the PTFE sleeve, place it onthe piston (13) and only then attach the O-ring (17).

4. Lightly grease the piston, PTFE sleeve and O-ring and reassemble the volume unit.

 he O-ring Seal Cuff Assembly is not accessible on 2, 10, 10Y and 20 μl models. If there is aTlack of tightness, the complete cylinder must be replaced.

4.3.3

Replacing the O-ring:

1. Unscrew the piston rod (21), remove the spring positioners (22, 23) and the spring (15).
2. If necessary, replace defective parts. Grease the O-ring (17) and cylinder (18) evenly.

26 262020 2032

11

2. Release the cylinder ring, pull out the spring (15) to reach the O-ring/PTFE sleeve.						

26

1, L
2, 5, 10 L
815 : 1, 2,815
5, :10815::models
1, other2, 5, 10L
allmodels
825L: 2, 10 815/825
L:815/825
all other825 : 2, 10models
815:825.0010Y)
815/825:825: 2,Circlip
10 L
1.All
Ejectorcushion12.
(except(except825.0010Y)(except825.0010Y)
1,2, 5, 10, 20 µL all othersizes

835 : all models
835 : all models
835:835
5 and10 mL: all models

855:spring
all sizes
23. Bottom
positioner

2. Ejection button 825:3. Spring washer 2, 10, 10Y, 20 µL

13. Piston

24 Ejection cap

14. Ring

25. Ejection sleeve

4. Smartie Cap

15. Spring

26. Ejection screw

5. Ejection spring

16. PTFE sleeve

27. Cover assembly

6. Counter unit (adjustable)

17. O-Ring (piston)

28. Arm

7. Dosing unit (ﬁx)18. Cylinder

29. Piston assembly

8. Handpiece

19. Ejector

30. Cylinder unit

9. Window

20 Ejection nut

31. Housing

10 Screws, handpiece (2x)21. Piston rod

32. O-ring (cone, 200 µl)

11. Ejection rod

22. Top spring positioner

33. Calibration slide4.4.1

31

Remove the volume unit (lower part):

1. Turn the ejection nut (20) and separate it from the ejection cap (24).
2. Unscrew the cylinder (18), press the operating button, then pull out the piston.

4.4.2

Changing the O-ring:

1.	Press down both clips of the cylinder (18) simultaneously and without exerting force and separate them fromthe ejection sleeve (25). Remove piston assembly.
2. Unscrew the piston rod (21), remove the spring positioners (22, 23) and the spring (15).
3. If necessary, replace defective parts. Grease the O-ring (17) and cylinder (18) evenly.
4. Reassemble the piston assembly, cylinder and ejection sleeve.
5. Screw the cylinder onto the pipette, press the operating button (B) down fully and lock the piston rod.

67

Instructions for Use Sarpette® M
4.5

Piston change, multi-channel Sarpette® M

A piston change in multi-channel pipettes, as well as the replacement of other defective parts, should always becarried out by their local service partner.

5

Instructions for Use Sarpette® M
7

Calibration

Each Sarpette® M was calibrated after production and individually tested. The control certificate supplied withthe pipette contains all data as well as its serial number. The performance data are checked with distilled waterat a constant (± 0.5 °C) room temperature between 20 and 25 °C, in accordance with ISO 8655.

FaultsProblemPossible causes

Solution

Tip is not fixedon coneTip ejector incorrectly positioned

Adjust the position of the tip ejector

Unsuitable tips

Use original or compatible tips

Piston sticks,
moves irregularlySoiled piston

Disassemble and clean piston(also grease 5 and 10 ml pipettes andmulti-channel pipettes)

Liquid is not
aspirated

Blocked cone

Dismantling and cleaning

Volumetric unit of pipette incorrectly
assembled

Assemble the lower part according to
the instruction manual

Contaminated protective filter for 5 and
10 ml pipettes

Replace protective filter

Different
liquid levelin tips ofmulti-channel pipettes

Lack of tightness

Check the fit of the pipette tips

Instrument does not
perform as required

Lack of tightness

Check whether cone, PTFE sleeve or
O-ring is defective

Unsuitable tips

Check compatibility and fit of pipettetips

Replace defective cone, PTFE sleeve orO-ring

Replace protective filter
Perform recalibration
Contaminated protective filter for 5 and
10 ml pipettes

Re-calibrate with appropriate liquid

7.1

In-lab calibration

With the Swift-Set calibration system, the pipettes can be quickly and safely adjusted to the correct volume asfollows:

Fig. 12Fig. 13• Remove the coloured cap (a) from the push button and remove the calibration screw (b).
• Pull off the calibration seal label (c) and set the calibration slide (d) to the position cal using a pipette tip orpointed object.
• Turn the calibration screw until the average measured volume appears on the digital display without
holding the push button.
• Move the calibration slide back into the lock position and while maintaining this position, press the push button
all the way down to the second stop.
• Release and place the coloured cap on the push button.
• After a few piston movements, check the result.
• Stick the recalibration seal label to the calibration slide.

Instrument calibrated incorrectly
Use of the instrument with viscous,
volatile or extremely temperaturecontrolled liquids

6

Sterilisation

8Warranty

The Sarpette® M is guaranteed against any manufacturing and material defects for the period of time mentioned
in the inspection certificate. Damage resulting from disregard of the operating and safety instructions orautoclaving at improper temperature as well as colour deviations of the materials are excluded from the warranty.
Repairs and replacement of spare parts do not extend the warranty period. If there are malfunctions that cannot
be rectified according to the above instructions, contact your SARSTEDT contact person.

The design of the Sarpette® M takes into account repeated sterilisation in an autoclave at 121 °C. (20 minutes).
For models 5 and 10 ml, remove the protective filter before autoclaving. Place the instrument horizontally in theautoclave, avoid direct contact with metal. Before use, check that the pipette is dry and completely cooled. For
model 5 and 10 ml, install new protective filter. Check the sealing and performance data regularly, but at least
after 50 autoclaving cycles. Re-tighten the volume unit if loose. A change in the colour of the material as well
as more difficult rotation of the coloured cap may occur after repeated autoclaving. Correct autoclaving andresulting sterility are the responsibility of the user.

89Instructions for Use Sarpette® M
9Performance data Sarpette® M

9.1

Single-channel Sarpette® M

10VolumeOrder no.

Volumerange

90.3100.002
90.3100.010

Instructions for Use Sarpette® M

Inaccuracy (E %)Min. vol.

Intermediate
vol.

Max. vol.

Min. vol.

Intermediate
vol.

Max. vol.

0.1 – 2 µl

0.002 µl<+/– 6.0 %<+/– 4.0 %<+/– 2.0 %< 5.0%< 3.3%< 1.5%

0.5 – 10 µl

0.01 µl<+/– 2.5 %<+/– 1.8 %<+/– 1.0 %< 1.8%< 1.2%< 0.5%

90.3100.020

2 – 20 µl

0.02 µl<+/– 2.5 %<+/– 1.8 %<+/– 1.0 %< 1.7%< 1.0%< 0.5%

90.3100.100

10 – 100 µl

0.01 µl<+/– 1.5 %<+/– 1.2 %<+/– 0.8 %< 1.0%< 0.6%< 0.2%

90.3100.200

20 – 200 µl

0.2 µl<+/– 1.5 %<+/– 1.1 %<+/– 0.6 %< 0.6%< 0.4%< 0.2%

90.3100.000

100 – 1000 µl

1 µl<+/– 1.5 %<+/– 1.0 %<+/– 0.5 %< 0.5%< 0.4%< 0.2%

90.3100.555

0.5 – 5 ml

0.01 ml<+/– 1.5 %<+/– 1.1 %<+/– 0.6 %< 0.6%< 0.5%< 0.3%

90.3100.111

1 – 10 ml

0.01 ml<+/– 1.5 %<+/– 0.7 %<+/– 0.5 %< 0.5%< 0.3%< 0.2%

Volumerange

Intermediate
vol.

Min. vol.

Max. vol.

Min.
vol.

Intermediate
vol.

Max.
vol.

0.5 – 10 µl

0.01 µl8<+/– 3.5 %1)<+/– 2.5 %<+/– 1.5 %< 3.0%< 2.0%< 1.0%

90.3108.200 20 – 200 µl

0.2 µl8<+/– 0.9 %1)<+/– 0.8 %<+/– 0.7 %< 0.6%< 0.5%< 0.3%

90.3108.300 30 – 300 µl

0.4 µl8<+/– 1.0 %1)<+/– 0.9 %<+/– 0.8 %< 0.6%< 0.5%< 0.3%

90.3112.010

0.5 – 10 µl

0.01 µl12<+/– 3.5 %1)<+/– 2.5 %<+/– 1.5 %< 3.0%< 2.0%< 1.0%

90.3112.200 20 – 200 µl

0.2 µl12<+/– 0.9 %1)<+/– 0.8 %<+/– 0.7 %< 0.6%< 0.5%< 0.3%

90.3112.300 30 – 300 µl

0.4 µl12<+/– 1.0 %1)<+/– 0.9 %<+/– 0.8 %< 0.6%< 0.5%< 0.3%

90.3108.010

The performance values were determined with distilled water at a constant temperature (± 0.5 °C) between 20 and 25 °C in accordance with
ISO 8655.

Volumerange

InnerPackaging

70.3010.xxx
70.3020.xxx
70.3021.xxx

90.3100.010

0.5 – 10 µl70.3010.xxx
70.3020.xxx
70.3021.xxx

90.3100.020

2–20 µl70.3030.xxx
70.3031.xxx

Single-channel pipettes

10 – 100 µl

1 pc./box

70.3030.xxx
70.3031.xxx

90.3100.200

20 – 200 µl

70.3030.xxx
70.3031.xxx

90.3100.000

100 – 1000 µl

70.3050.xxx
70.3060.xxx

90.3100.555

0.5 – 5 ml

70.1183.102
70.1183.002

90.3100.111

1 – 10 ml

70.1187.102
70.1187.002

90.3108.010

0.5 – 10 µl70.3010.xxx
70.3020.xxx
70.3021.xxx

90.3108.200

8-channel pipettes

20 – 200 µl

1 pc./box

70.3030.xxx
70.3031.xxx

90.3108.300

30 – 300 µl

70.3030.xxx
70.3031.xxx
70.3040.xxx

90.3112.010

0.5 – 10 µl70.3010.xxx
70.3020.xxx
70.3021.xxx

90.3112.200

measured at 10% nominal volume

Suitable SARSTEDT
pipette tips

0.1 – 2 µl

Imprecision (CV %)p/n 03-0-0002-0197

Order no.

Inaccuracy (E %)NumberIncrement
ofchannels

Description

90.3100.002

90.3100.100

Multi-channel Sarpette® M8 / M12
Volume1)

Order no.

Imprecision (CV %)Increment

9.2

Ordering information

20 – 200 µl
30 – 300 µl

1 pc./box

70.3030.xxx
70.3031.xxx
70.3030.xxx
70.3031.xxx
70.3040.xxx

MNL_20_051_0000_200_NF

Technical modifications reserved

90.3112.300

12-channel pipettes

SARSTEDT AG & Co. KGP.O. Box 12 20 · D-51582 Nümbrecht
Phone: +49 2293 305 0
Fax: +49 2293 305 3450
Customer Service Germany
Phone 0800 0 83 305 0
export@sarstedt.com
www.sarstedt.com

1011