Get yourcounting timeback.

Cellaca® MX high-throughput cell counterForget cell counting
as you know it
Cellaca MX changes everything. Count 24 samples in2.5 minutes or less — skip the sign-up sheet and thelong waits. Get your all-day, multi-scientist experiments
done in 1.5 hours with one pair of hands. Analyze
low‑volume primary cell samples, and count only thecells you’re looking for.
Cellaca MX lets you do high-speed,
high-throughput counting.
• Analyze cell lines or primary cells like PBMCs,
T cells and tumor digests

Carousel counter• Batch up to 24 samples at a time for cell count,viability by Trypan blue or AO/PI• Assess sample quality and counts with higher
accuracy using image cytometry• Move cells on to next steps faster to minimize
variability in results

Cellaca MX

Count the right cells every time
Cellaca MX gives you the flexibility to run assays that
fit your sample and experimental conditions best. Usebrightfield to check viability. Switch to fluorescenceto get specific details on cell health or analyze
heterogeneous populations.1 Cellaca MX identifies
only your cells of interest — tissue debris and red
blood cells won’t artificially change the count. Move on
to next steps knowing the results you’ll get there arebased on an accurate count.

Brightfield and fluorescent images of PBMCs. In brightfield,
white blood cells, platelets, and red blood cells are seen.
Nuclear fluorescent staining using both green and red channels
accurately identifies only the white blood cells.

1 Accurate measurement of peripheral blood mononuclear cell concentration using image cytometry to eliminate RBC‑induced counting error,
Chan. et.al, J Immunol Methods, 388:25–32, 2013.

2

Get your time back
Save time and resources every time with Cellaca MX.

Experiment: 50 mice, 3 samples per mouse, 150 samples

Single slide counter

Cellaca MXSingle slide counter

Cellaca MX

4 researchers

1 researcher

1 count/run

24 counts/run

12 hours hands-on time

1.5 hours hands-on time

Cellaca MX gets the project done 10.5 hours faster, and saves the time of 3 researchers.

Analyze primary cells
Getting accurate information on cell health is next to impossible with other systems as they often need 350 µL ofsample or more. Cellaca MX only needs 25 µL, which makes analysis of tumor biopsy tissue, mouse splenocytes,
tumor infiltrating lymphocytes (TILs) and more completely possible. You’ll also have enough sample left for
downstream analyses.

Brightfield

Acridine Orange

Propidium Iodide

AO/PI Overlay

Human PBMC sample stained with Acridine Orange/Propidium iodide (AO/PI) and imaged using brightfield and fluorescence on Cellaca MX.

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3Everything you need for your assays
Cellaca MX comes with everything you need to start counting on day one, including software,consumables, and reagents. As your experimental needs change, choose from a wide range of dyesthat are tested and validated for use on Cellaca MX.

System specifications
Description

Specification

Samples per run

24

Counting speed per sample

2-17 sec (assay dependent)

Volume per sample

25-200 μL

Imaging methods

Brightfield, Trypan Blue, Fluorescence

Sample types

Cultured cell lines, blood samples, primary cell samples,
digested tissue cells, isolated and purified cells

Compatible dyes and fluorophores

Hoechst, DAPI, PE, APC, FITC, CFDA, Calcein AM, 7AADExcitation LED

365, 470, 527 and 620 nm

Emission filters (bandpass, center wavelength)452, 534, 605, 655 and 692 nm

Plate format

SBS Format

Automation ready

Available API for Robotic Integration

Dimensions and weight

13 in x 13 in x 16 in (33 cm x 33 cm x 41 cm) 42 lb (19 kg)

For research use only. Not approved for diagnostic or therapeutic use.

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940 Winter Street
Waltham, MA 02451 USA
www.revvity.com
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