HTX M5 Sprayer™
Tissue MALDI Sample Preparation System

HTX M5 Sprayer™ Tissue MALDI Sample Preparation System

HTX M5 Sprayer™
System is an automated
MALDI matrix deposition
system offeringhigh reproducibility andsuperior data quality for
Mass Spectrometry Imaging
HTX M5 SPRAYER™

The HTX M5 Sprayer™ is an easy-to-use, versatile
spraying system that provides automated process for Sample
Preparation in Mass Spectrometry Imaging.

Key Characteristics

The proprietary spray technology of the HTX M5 Sprayer™
guarantees a very fine, uniform and consistent matrix
coating crucial for high-resolution imaging and relative
quantification of analytes.

◆ Proprietary technology providing very small matrix

droplets ( <5 microns)◆ High flow rate and fast sample prep (2 to 18 minutesper slide)◆ Highly consistent matrix deposition across entire

sample area (+/- 3% by weight)◆ Unique use of temperature and nitrogen flow toThe unique ability to control liquid and propulsion gas
temperature creates a fine solution mist that can bedeposited in a precise and adjustable pattern over all or partof any MALDI plate.
Spray characteristics (wet or dry) are easily adjustable via
the intuitive operator interface. Users can create and savemethods for reproducible operation.

control evaporation rate and matrix crystal formation◆ More than 30 valided protocols covering trypsin andmost matrices (e.g.: SA, CHCA, DHB, DAN, 9-AA,DHA, CMBT, THAP)◆ Validated protocols for Trypsin digestion of FFPE
◆ Continuous matrix coverage as needed forhigh-resolution imaging◆ Rugged operation and easy clean-up

Slow Matrix
Solution
Evaporation
Cycle

PROMOTESPROMOTES

Desorption
Co-crystalization

Delocalization

IMPROVESMS SignalIntensity

WORSENS

ImageResolution© 2011 HTX Technologies, LLC

MATRIX DEPOSITION CHALLENGE

Addressing the Matrix Deposition Challenge
The main challenge when preparing samples for MALDI
Mass Spectrometry Imaging is to balance the positiveeffects of the matrix solution penetrating the tissue and cocrystallizing with the analytes, and the negative effects
of analytes delocalization.

contribute to a greater user experience and expanded
process capabilities including:

The HTX M5 Sprayer™ builds on the proven capabilities
of its predecessor, the HTX TM-Sprayer™, to offer
even greater performance. The all-new M5 chassis,
high velocity stage and heated sample holder drawer◆ Faster and drier deposition capability◆ On-tray trypsin digestion capability(with optional humidity chamber)◆ On-tray sample re-hydration(with optional humidity chamber)On the next page view a comparison of the two models.

SUCCESSFUL SPRAY DEPOSITION OF
2,4,6-TRIHYDROXYACETOPHENONE MALDI MATRIX
2,4, 6 Trihydroxyacetophenone or THAP offers great potential for MALDI
analysis and Imaging of lipids, acidic glycans and glycol-peptides innegative ion mode, but its deposition by spray is challenging due to thetendency of THAP to form hydrates. When deposited by spray undernormal conditions, the hydrated solution results in a liquid film layer thatdries very slowly and forms a non-uniform coating. In the example below
we devised a two steps process to deposit THAP successfully and createhigh resolution MS images.
Taking advantage of the high velocity of the XY stage of the HTX M5
Sprayer, the first four layers are deposited in super dry mode to create aseeding layer, then the next four layers, better optimized for extraction, aredeposited on top resulting in a very good balance of analytes extraction,PROTOCOLFirst 4 passes:
Matrix:

2,4,6-THAP at 1mg/ml in 100% Acetone

require rehydration to produce a high intensity spectrum.

Flow Rate:

0.050 ml/min

Velocity:

2,000 mm/min

Data and images courtesy of Dr. Junhai Yang, Vanderbilt University.

Temperature:

30°C

high signal intensity and limited spatial delocalization. This protocol did not

Track Spacing: 1.5 mm
Next 4 passes:
Matrix:

2,4,6-THAP at 11.1mg/ml
in 66% Chloroform, 34% Methanol

m/z 806.3 +/- 0.5Da

m/z 885.3 +/- 0.5Da

Flow Rate:

0.100 ml/min

Velocity:

3,600mm/min

Temperature:

30°C

Track spacing:

1.5 mmINSTRUMENTS

m/z 888.4 +/- 0.5Da

m/z 906 Da +/- 0.5 Da

Sample Prep:

Prototype HTX M5 Sprayer

Analysis:

Bruker rapifleX TissueTyper

Acquisition raster: 15µm

HTX M5-Sprayer™ Tissue MALDI Sample Preparation System

HTX MALDI Sample Preparation Systems: M3 vs. M5
Product Name
Front view

HTX TM-Sprayer

HTX M5 Sprayer

M3 chassis16.5”W x 13”H x 13”D40 lbs. (18Kg)
180 x 140mm
NoNoCup

M5 chassis11.5”W x 16.3”H x 14”D35 lbs. (16Kg)
180 x 140mm
Yes
Yes
Trough gutter EZ in/out

XY stage
Maximum stage velocity
Nozzle height

1,350 mm/min
Fixed (40mm)

5,600 mm/min
Adjustable (25, 40, 52mm)

Heated Spray Nozzle

24V 40W RED Nozzle

Same

Left panel
2 COM Ports
XY, Temperature

Top panel
4 COM Ports
XY, Temp1, Temp2, Pump

TMSP CS 4.1

TMSP CS 5.1

Methods.xml file offering
30+ validated protocols

Expended but fully compatible withall previous methods.

ChassisInstrument footprint
WeightAvailable spray area
Drawer loading
Heated tray
Waste/Drain

Electronics/SoftwareLocation
COM channels

Software version
Protocols

HTX TECHNOLOGIES PRODUCTS ARE FOR RESEARCH USE ONLY.

NOT FOR USE IN DIAGNOSTICS PROCEDURES.

HTX M5 Sprayer™ is available worldwide exclusively from HTX Technologies, LLC.
To request further information contact:

Alain Creissen
Imaging Product Manager, HTX Technologies
acreissen@htximaging.com
HTX Technologies offers innovative sample preparation systems for advanced
analytical platforms. Our integrated workflow solutions include user training,instruments, software, consumables and method development services.

PO Box 16007, Chapel Hill, NC 27516 USA
Tel +1-919-928-5688

Fax +1-919-928-5153

info@htximaging.com

www.htximaging.com