学術論文 (＊ Corresponding author)
2023 (R5)1) Ultrahigh-throughput screening of Trichoderma reesei strains capable of carbon
catabolite repression release and cellulase hyperproduction using a microfluidic
droplet platform,
Xuan Chinh Luu, Yosuke Shida, Yoshiyuki Suzuki, Daiki Kuwahara, Takeshi
Fujimoto, Yuka Takahashi, Naomi Sato, Akihiro Nakamura, Wataru Ogasawara*,
Bioscience, Biotechnology, and Biochemistry, zbad108 (online), (2023.8.8)2022 (R4)2) Prediction of ethanol fermentation under stressed conditions using yeast
morphological data,
Kaori Itto-Nakama, Shun Watanabe, Shinsuke Ohnuki, Naoko Kondo, Ryota
Kikuchi, Toru Nakamura, Wataru Ogasawara, Ken Kasahara, Yoshikazu Ohya,
Journal of Bioscience and Bioengineering, 135(3), 210-216,(2023.1.13),
https://doi.org/10.1016/j.jbiosc.2022.12.008

3) A microfluidic device for simultaneous detection of enzyme secretion and elongationof a single hypha,Ayaka Itani, Yosuke Shida, Wataru Ogasawara*,
Frontiers in Microbiology,14, 1125760, (2023.03.03)
https://doi.org/10.3389/fmicb.2023.1125760

4) LsSpt23p is a regulator of triacylglycerol synthesis in the oleaginous yeast Lipomyces

starkeyi,
Hiroaki Takaku, Haruka Kazama, Rikako Sato, Kazuki Mori, Satoshi Ara, Koji Ishiya,
Tomohiko Matsuzawa, Katsuro Yaoi, Hideo Araki, Yosuke Shida, Wataru Ogasawara,
Kosuke Tashiro, Satoru Kuhara, Harutake Yamazaki, Sachiyo Aburatani,
Applied Microbiology and Biotechnology, 107(4), 1269-1284, (2023.01.17)
https://doi.org/10.1007/s00253-023-12361-2

5) The monitoring of oil production process by deep learning based on morphology inoleaginous yeasts,
Yukina Kitahara, Ayaka Itani, Kazuma, Ohtomo, Yosuke Oda, Yuka Takahashi,
Makoto Okamura, Mizue Mizoshiri, Yosuke Shida, Toru Nakamura, Ryosuke
Harakawa, Masahiro Iwahashi, Wataru Ogasawara*,
Applied Microbiology and Biotechnology, 107, 915-929, (2022.12.28)
https://doi.org/10.1007/s00253-022-12338-7

6) A novel high-throughput approach for transforming filamentous fungi employing adroplet-based microfluidic platform,
Xuan Chinh, Yosuke Shida, Yoshiyuki Suzuki, Naomi Sato, Akihiro Nakamura,
Wataru Ogasawara*,
New Biotechnology, 72, 149-158, (2022.12.25)
https://doi.org/10.1016/j.nbt.2022.11.003

7) Using gel microdroplets to develop a simple high-throughput screening platform foroleaginous microorganisms,
Yuma Tanaka, Akihiro Nakamura, Yoshiyuki Suzuki, Kodai Maruta, Yosuke Shida,
Wataru Ogasawara*,
Journal of Biotechnology, 358 (10), 46-54, (2022,11.10)
https://doi.org/10.1016/j.jbiotec.2022.08.016

8) A real-time monitoring system for automatic morphology analysis of yeast
cultivation in a jar fermenter,
Yukina Kitahara, Ayaka Itani, Yosuke Oda, Makoto Okamura, Mizue Mizoshiri,
Yosuke Shida, Toru Nakamura, Ken Kasahara, Wataru Ogasawara*,
Applied Microbiology and Biotechnology, 106 (12), 4683-4693, (2022.6.10),
https://doi.org/10.1007/s00253-022-12002-0

9) 7-Aminocoumarin-4-acetic Acid as a Fluorescent Probe for Detecting Bacterial
Dipeptidyl Peptidase Activities in Water-in-Oil Droplets and in Bulk,
Akihiro Nakamura, Nobuyuki Honma, Yuma Tanaka, Yoshiyuki Suzuki, Yosuke
Shida, Yuko Tsuda, Koushi Hidaka*, and Wataru Ogasawara*
Analytical Chemistry, 94, Article number: 2416-2424, (2022.2.8),
https://doi.org/10.1021/acs.analchem.1c04108

2021 (R3)10) Disruption of alpha-tubulin releases carbon catabolite repression and enhances
enzyme production in Trichoderma reesei even in the presence of glucose,
Nozomu Shibata, Hiroshi Kakeshita, Kazuaki Igarashi, Yasushi Takimura, Yosuke
Shida, Wataru Ogasawara, Tohru Koda, Tomohisa Hasunuma, Akihiko Kondo
Biotechnology for Biofuels, 14, Article number: 39, (2021.2.8),
https://doi.org/10.1186/s13068-021-01887-0
11) Structural basis for an exceptionally strong preference for asparagine residue at theS2 subsite of Stenotrophomonas maltophilia dipeptidyl peptidase 7,
Akihiro Nakamura, Yoshiyuki Suzuki, Yasumitsu Sakamoto, Saori Roppongi,
Chisato Kushibiki, Natsuri Yonezawa, Masato Takahashi, Yosuke Shida, Hiroaki
Gouda, Takamasa Nonaka, Nobutada Tanaka, Wataru Ogasawara*,
Scinentific reports, 11 , Article number: 7929, (2021.4.12)
https://doi.org/10.1038/s41598-021-86965-x

2020(R2)12) Involvement of Xyr1 and Are1 for Trichodermapepsin Gene Expression in Response
to Cellulose and Galactose in Trichoderma reesei,
Daranagama ND, Yoshiyuki Suzuki, Yosuke Shida, Wataru Ogasawara*,
Current Microbiology ,77, 1506-1517, April 1 (2020), doi:10.1007/s00284-020-01955-y13) Functional analysis of a novel lytic polysaccharide monooxygenase from
Streptomyces griseus on cellulose and chitin,
Kazuki Sato, Daisuke Chiba, Sayaka Yoshida, Mayu Takahashi, Kazuhide Totani,
Yosuke Shida, Wataru Ogasawara, Yuko S.Nakagawa,International Journal of Biological Macromolecules, 164, 2085-2091, (2020),
https://doi.org/10.1016/j.ijbiomac.2020.08.015

2019(R1)
14) The Crystal Structure of Peptidase Toward Drug Discovery,
Sakamoto, Yasumitsu, Saori Roppongi, Yoshiyuki Suzuki, Tsukasa Ishihara, Koushi
Hidaka, Akihiro Nakamura, Nobuyuki Honma, Wataru Ogasawara, and Nobutada
Tanaka，International Journal of Microgravity Science and Application, 36(1), 360106-1-8,(2019)

15) Characterization of earthworm α-amylases for dietary supplement development andbiomass utilization,
Shin-ichi Akazawa, Yuki Ikarashi, Keisuke Yokoyama, Yosuke Shida, Wataru
Ogasawara,
Environmental Science and Pollution Research,27,33458-33463, (2019)16) Fragment-Based Discovery of the First Nonpeptidyl Inhibitor of an S46 Family
Peptidase,
Sakamoto, Yasumitsu, Yoshiyuki Suzuki, Akihiro Nakamura, Yurie Watanabe,
Mizuki Sekiya, Saori Roppongi, Chisato Kushibiki, Ippei Iizuka, Osamu Tani,
Hitoshi Sakashita, Koji Inaka, Hiroaki Tanaka, Mitsugu Yamada, Kazunori Ohta,
Nobuyuki Honma, Yosuke Shida, Wataru Ogasawara, Mayumi Nakanishi-Matsui,
Takamasa Nonaka, Hiroaki Gouda, and Nobutada Tanaka,
Sci. Rep. 9, 13587 (2019).

17) Skin-deep Surface Patterning of Calcite,
David C Green, Yosuke Shida, Nobuyuki Honma, Mark A Holden, Yi-Yeoun Kim,
Alexander N Kulak, Wataru Ogasawara, Fiona C Meldrum,
Chemistry of Materials,31, 8725-33,(2019)18) Hiroaki Takaku, Atsumi Miyajima, Haruka Kazama, Rikako Sato, Satoshi Ara,
Tomohiko Matsuzawa, Katsuro Yaoi, Hideo Araki, Yosuke Shida, Wataru
Ogasawara, Harutake Yamazaki,
A novel electroporation procedure for highly efficient transformation of Lipomyces
starkeyi, Journal of Microbiological Methods,169,105816, (2019)19) Pham Khanh Dung, Yosuke Shida, Atsushi Miyata, Takeru Takamizawa, Yoshiyuki
Suzuki, Satoshi Ara, Harutake Yamazaki, Kazuo Masaki, Kazuki Mori, Sachiyo
Aburatani, Hideki Hirakawa, Kosuke Tashiro, Satoru Kuhara, Hiroaki Takaku,
Ogasawara Wataru*,
Effect of light on carotenoid and lipid production in the oleaginous yeast

Rhodosporidium toruloides, Bioscience Biotechnology and Biochemistry, 87(7), 15011512, Mar 19(2020), https://doi.org/10.1080/09168451.2020.1740581

2018（H30）
20) Crystal structures of a bacterial dipeptidyl peptidase IV reveal a novel substrate
recognition mechanism distinct from that of mammalian orthologues,
Saori Roppongi, Yoshiyuki Suzuki, Chika Tateoka, Mayu Fujimoto, Saori Morisawa,
Ippei Iizuka, Akihiro Nakamura, Nobuyuki Honma, Yosuke Shida, Wataru
Ogasawara, Nobutada Tanaka, Yasumitsu Sakamoto & Takamasa Nonaka, Scientific
Reports 8(1), Article number:2714, (2018)21) Engineering of the Trichoderma reesei xylanase3 promoter for efficient enzyme
expression,
Hiroki Hirasawa, Koki Shioya, Takanori Furukawa, Shuji Tani, Jun-ichi Sumitani,
Takashi Kawaguchi, Yasushi Morikawa, Yosuke Shida, Wataru Ogasawara*,
Applied Microbiology and Biotechnology, 102(6), 2737-2752, (2018)22) High-pressure tolerance of earthworm fibrinolytic and digestive enzymes,
Shin-ichi Akazawa, Haruka Tokuyama, Shunsuke Sato, Toshinori Watanabe, Yosuke
Shida, Wataru Ogasawara,
Journal of Bioscience and Bioengineering, 125(5), 155-159, (2018)23) Nitric oxide production from nitrite reduction and hydroxylamine oxidation bycopper-containing dissimilatory nitrite reductase (NirK) from an aerobic ammoniaoxidizing archaeon, Nitrososphaera viennensis,
Shun Kobayashi, Daisuke Hira, Keitaro Yoshida, Masanori Toyofuku, Yosuke Shida,
Wataru Ogasawara, Takashi Yamaguchi, Nobuo Araki, Mamoru Oshiki,
Microbes and Environments,33(4), doi:10.1264/jsme2.ME18058, (2018)24) Proteolytic analysis of Trichoderma reesei in celluase-inducing condition reveals arole for trichodermapepsin (TrAsP) in cellulase production,
Nayani Dhanushka Daranagama, Koki Shioya, Masahiro Yuki, Haruna Sato, Yuki
Ohtaki, Yoshiyuki Suzuki, Yosuke Shida, Wataru Ogasawara*,
Journal of Industrial Microbiology & Biotechnology, Vol.46(6), 831-842, (2019)
DOI: 10.1007/s10295-019-02155-9 (2018)25) Cellulase productivity of Trichoderma reesei mutants developed in Japan varies
with varying pH conditions,Hiroki Hirasawa, Koki Shioya, Kazuki Mori, Kosuke Tashiro, Sachiyo Aburatani,
Yosuke Shida, Satoru Kuhara, Wataru Ogasawara*,
Journal of Bioscience and Bioengineering, 128(3),264-273, (2019)26) Purification, Cloning, Functional Expression, Structure, and Characterization of a
Thermostable β-Mannanase from Talaromyces trachyspermus B168 and Its
Efficiency in Production of Mannooligosaccharides from Coffee Wastes,
Kentaro Suzuki, Mari Michikawa, Haruna Sato, Masahiro Yuki, Kei Kamino,
Wataru Ogasawara, Shinya Fushinobu, Satoshi Kaneko,
The Japanese Society of Applied Glycoscience,65, 13-21, (2018)

2017（H29）
27) Periplasmic form of dipeptidyl aminopeptidase IV from Pseudoxanthomonas

mexicana WO24: purification, kinetic characterization, crystallization and X-ray
crystallographic analysis, Saori Roppongi, Chika Tateoka, Mayu Fujimoto, Ippei
Iizuka, Saori Morisawa, Akihiro Nakamura, Nobuyuki Honma, Yoshiyuki Suzuki,
Yosuke Shida, Wataru Ogasawara, Nobutada Tanaka, Yasumitsu Sakamoto andTakamasa Nonaka, Acta Crystallographica Section F,73(11),601-606, (2017)

2016（H28）
28) (Review)Deciphering the molecular mechanisms behind cellulase production in

Trichoderma reesei, the hyper-cellulolytic filamentous fungus, Shida Y., Furukawa
T.,

Ogasawara W*., Bioscience, Biotechnology, and Biochemistry, 80(9), 1712-

29, (2016)29) 糖非発酵グラム陰性細菌由来新規ジペプチド産生酵素の構造と機能, 阪本 泰光, 野中
孝昌, 鈴木 義之, 小笠原 渉, 田中 信忠, 日本結晶学会誌, 58(5), 221-7, (2016)30) 微生物によるキノコ廃菌床からの化学工業原料生産システム開発, 高久 洋暁, 山崎
晴丈, 志田洋介, 小笠原 渉, 環境技術, 45(5),239-245,（2016）

31) Efficient gene targeting in non-homologous end-joining-deficient Lipomyces starkeyi
strains, Oguro Y., Yamazaki H., Ara S., Shida Y., Ogasawara W., Takagi M., Takaku
H., Current Genetics, Published online: 20 Feb. (2017)

2015(H27)
32) The impact of a single-nucleotide mutation of bgl2 on cellulase induction in a

Trichoderma reesei mutant, Y. Shida, K. Yamaguchi, M. Nitta, A. Nakamura, M.
Takahashi, S. Kidokoro, K. Mori, K. Tashiro, S. Kuhara, T. Matsuzawa, K. Yaoi, Y.
Sakamoto, N. Tanaka, Y. Morikawa and W. Ogasawara*, Biotechnol Biofuels, 8:230,(2015),

33) A high performance Trichoderma reesei strain that reveals the importance of
XylanaseIII in cellulosic biomass conversion, H. Nakazawa, T. Kawai, N. Ida, Y.
Shida, K. Shioya, Y. Kobayashi, H. Okada, S. Tani, J. Sumitani, T. Kawaguchi, Y.
Morikawa and W. Ogasawara*, Enzyme. Microb. Technol., in press, 82, 89-95, (2015)34) Characterization of two endoglucanases for the classification of the earthworm.
Eisenia fetida Waki, SI. Akazawa*, Y. Ikarashi, K. Yarimizu, T. Kobayashi, H.
Nakazawa, W. Ogasawara and Y. Morikawa, Biosci. Biotechnol. Biochem., 21:1-12,(2015)

35) On the Mechanism of Cuprate Crystal Growth: The Role of Mixed Metal Carbonates,
D. C. Green, R. Boston, S. Glatzel, M. R. Lees, S. C. Wimbush, J. Potticary, W.
Ogasawara and S. R. Hall*, Adv. Funct. Mater., 25 (29):4700-7, (2015)36) Ultrastructure of the cellulolytic fungus Trichoderma reesei, Y. Shida, A. Morikawa,R. Tamochi, N. Nango, H. Okada, M. Osumi and W. Ogasawara*, Plant Morphology,
in press, (2015)37) Structural and mutational analyses of dipeptidyl peptidase 11 from Porphyromonas

gingivalis reveal the molecular basis for strict substrate specificity, Y. Sakamoto, Y.
Suzuki, C. Tateoka, S. Roppongi, M. Fujimoto, K. Inaka, H. Tanaka, M. Yamada, K.
Ohtake, H. Gouda, T. Nonaka, W. Ogasawara* and N. Tanaka*, Sci. Rep. 5, Article
number:11151, (2015).

38) Heterologously expressed Aspergillus aculeatus β-glucosidase in Saccharomyces

cerevisiae is a cost-effective alternative to commercial supplementation of βglucosidase in industrial ethanol production using Trichoderma reesei cellulases, T.
Treebupachatsakul, H. Nakazawa, H. Shinbo, H. Fujikawa, A. Nagaiwa, N. Ochiai,
T. Kawaguchi, M. Nikaido, K. Totani, K. Shioya, Y. Shida, Y. Morikawa, W.
Ogasawara* and H. Okada, J. Biosci. Bioeng., in press, (2015)39) Utilization of recombinant Trichoderma reesei expressing Aspergillus aculeatus βglucosidase I (JN11) for a more economical production of ethanol from lignocellulosic
biomass, T. Treebupachatsakul, K. Shioya, H. Nakazawa, T. Kawaguchi, Y.
Morikawa, Y. Shida, W. Ogasawara* and H. Okada, J. Biosci. Bioeng., in press,(2015)

40) Multicopy integration and expression of heterologous genes in the oleaginous yeast,

Lipomyces starkeyi, Y. Oguro, H. Yamazaki, Y. Shida, W. Ogasawara, M. Takagi and
H. Takaku*, Biosci. Biotechnol. Biochem., 79(3):512-5, (2015)41) Crystallization and preliminary X-ray crystallographic studies of dipeptidyl
peptidase 11 from Porphyromonas gingivalis, Y. Sakamoto, Y. Suzuki, I. Iizuka, C.
Tateoka, S. Roppongi, M. Fujimoto, H. Gouda, T. Nonaka, W. Ogasawara and N.
Tanaka*, Acta Crystallogr. F., 71(Pt 2):206-10, (2015)

2014（H26）
42) Effects of clbR overexpression on enzyme production in Aspergillus aculeatus varydepending on the cellulosic biomass degrading enzyme species, E. Kunitake, A.
Kawamura, S. Tani, S. Takenaka , W. Ogasawara, J. Sumitani and T. Kawaguchi*,

Biosci. Biotechnol. Biochem., 79(3):488-95, (2014).
43) Formation of superconducting yttrium barium copper oxide using sulphurcontaining templates, R. Boston, K. Awaya, T. Nakayama, W. Ogasawara and S. R.
Hall*, RSC Adv., 26824-28, (2014)44) S46 Peptidases are the First Exopeptidases to be Members of Clan PA, Y. Sakamoto,Y. Suzuki , I. Iizuka, C. Tateoka, S. Roppongi, M. Fujimoto, K. Inaka, H. Tanaka, M.

Masaki, K.Ohta, H. Okada , T. Nonaka, Y. Morikawa, K. T. Nakamura, W.
Ogasawara* and N.Tanaka*, Sci. Rep. 4, Article number: 4977, (2014)45) Identification of the Catalytic Triad of Family S46 Exopeptidases, Closely Related toClan PA Endopeptidases, Y. Suzuki, Y. Sakamoto, N. Tanaka, H. Okada, Y.
Morikawa and W. Ogasawara*, Sci. Rep. 4, Article number:4292, (2014)46) Crystallization and preliminary X-ray crystallographic studies of dipeptidyl
aminopeptidase BII from Pseudoxanthomonas mexicana WO24, Y. Sakamoto, Y.
Suzuki, I. Iizuka, C.Tateoka, S.Roppongi, H.Okada, T.Nonaka, Y. Morikawa, K.
Nakamura, W. Ogasawara and N. Tanaka*, Acta Crystallogr. F., (70):221-224, (2014)

2013（H25）
47) NaOH処理を利用したきのこ廃菌床からの資源回収, 岡部陽平，齋藤耕平，中村明靖，
幡本将史，志田洋介，小笠原 渉，福田雅夫，若山 樹，今田美郎，山口隆司*, 土木学会論文集 G(環境), 69巻7号, [III-145-III-150], (2013)48) A comprehensive analysis of the effects of the main component enzymes of cellulase
derived from Trichoderma reesei on biomass saccharification, T. Kawai, H.
Nakazawa, H. Ida, H. Okada, W. Ogasawara, Y. Morikawa and Y. Kobayashi*, J.

Ind. Microbiol. Biotechnol., 40(8):805-810, (2013)49) Single Nucleotide Polymorphism Analysis of a Trichoderma reesei Hyper-Cellulolytic
Mutant Developed in Japan, J. O. Porciuncula, T. Furukawa, K. Mori, Y. Shida, H.
Hirakawa, K. Tashiro, S. Kuhara, S. Nakagawa, Y. Morikawa and W. Ogasawara*,

Biosci. Biotechnol. Biochem., 23;77(3):534-43, (2013)（2013論文賞）

50) Identification of major facilitator transporters involved in cellulase production
during lactose culture of Trichoderma reesei PC-3-7, J. O. Porciuncula, T. Furukawa,Y. Shida, K. Mori, S. Kuhara, Y. Morikawa and W. Ogasawara*, Biosci. Biotechnol.

Biochem., 77(5):120992-1-9, (2013)51) Ethanol production from high cellulose concentration by the basidiomycete fungus

Flammulina velutipes, T. Maehara, H. Ichinose, T. Furukawa, W. Ogasawara, K.
Takabatake and K. Kaneko*, Fungal Biology, 117 (3):220-26, (2013)

2012（H24）
52) Analysis of the saccharification capability of high-functional cellulase JN11 forvarious pretreated biomasses through a comparison with commercially available
counterparts, T. Kawai, H. Nakazawa, N. Ida, H. Okada, S. Tani, J. Sumitani, T.
Kawaguchi, W. Ogasawara, Y. Morikawa and Y. Kobayashi*, J. Ind. Microbiol.

Biotechnol., 39(12):1741-1749, (2012)53) A New Zn(II)2Cys6-Type Transcription Factor BglR Regulates beta-glucosidase
Expression in Trichoderma reesei, M. Nitta, T. Furukawa, Y. Shida, K. Mori, S.
Kuhara, Y. Morikawa and W. Ogasawara*, Fungal Genet. Biol., 49(5):388-97, (2012),
DOI:10.1016/j.fgb.2012.02.009

54) Hyphal surface architecture and cell morphology of Trichoderma reesei, M. Nitta, Y.
Shida, H. Okada, M. Osumi and W. Ogasawara*, J. Elect. Micros., 61(3):187-92,(2012)

55) Biotemplated synthesis of catalytic Au–Pd nanoparticles, S. R. Hall*, A. M. Collins,
N. J. Wood, W. Ogasawara, M. Morad, P. J. Miedziak, M. Sankar, D. W. Knight and
G. J. Hutchings, RSC Adv., 2(6), 2217-2220, (2012)

56) Construction of a recombinant Trichoderma reesei Strain Expressing Aspergillus

aculeatus β-glucosidase 1 for Efficient Biomass Conversion, H. Nakazawa, T. Kawai,N. Ida, Y. Shida, Y. Kobayashi, H. Okada, S. Tani, J. Sumitani, T. Kawaguchi, Y.
Morikawa and W. Ogasawara*, Biotechnol. Bioeng., 109(1):92-9, (2012)57) High Jc in a biopolymer-mediated synthesis of YBa2Cu3O7_, S. R. Hall*, C. F. Hall,
K. Hansberry, S. C. Wimbush, Y. Shida and W. Ogasawara, Supercond. Sci. Technol.,25(3):035009, (2012)

2011（H23）58) Biotemplated synthesis of superconducting plate-like YBa2Cu3O7-δ using
oligosaccharides, S.R. Hall*, S.C. Wimbush, Y. Shida and W. Ogasawara, Chem.

Phys. Lett., 507(1-3):144-150, (2011)59) General Route to Functional Metal Oxide Nanosuspensions, Enzymatically
Deshelled Nanoparticles, and Their Application in Photocatalytic Water Splitting, C.
Neudeck, Y. Kim, W. Ogasawara, Y. Shida, F. Meldrum and D. Walsh*, small.,7(7):869-73, (2011)

2010（H22）
60) Crystallization and preliminary X-ray crystallographic studies of an exo-beta-Dglucosaminidase from Trichoderma reesei, Y. Sakamoto, M. Ike, N. Tanaka, Y.
Suzuki, W. Ogasawara, H. Okada, T. Nonaka*, Y. Morikawa and K.T. Nakamura,Acta Crystallogr. F, Struct. Biol. Cryst. Commun., 1;66(Pt 3):309-12, (2010)

2009（H21）61) Identification of specific binding sites for XYR1, a transcriptional activator ofcellulolytic and xylanolytic genes in Trichoderma reesei, T. Furukawa, Y. Shida, N.
Kitagami, K. Mori, M. Kato, T. Kobayashi, H. Okada, W. Ogasawara and Y.
Morikawa*, Fungal. Genet. Biol., 46, 564-74, (2009)62) Evaluation and characterization of Trichoderma reesei cellulase and xylanasepromoters, Z. Rahman, Y. Shida, T. Furukawa, Y. Suzuki, H. Okada, W. Ogasawara
and Y. Morikawa*, Appl. Microbiol. Biotechnol., 82, 899-908, (2009)63) Application of Trichoderma reesei cellulase and xylanase promoters throughhomologous recombination for enhanced production of extracellular beta-glucosidase
I, Z. Rahman, Y. Shida, T. Furukawa, Y. Suzuki, H. Okada, W. Ogasawara and Y.
Morikawa*, Biosci. Biotechnol. Biochem., 73, 1083-9, (2009)64) Directed evolution of endoglucanase III (Cel12A) from Trichoderma reesei, H.
Nakazawa, K. Okada, T. Onodera, W. Ogasawara, H. Okada and Y. Morikawa*,

Appl. Microbiol. Biotechnol., 83, 649-57,（2009）

2008（H20）
65) Characterization of the catalytic domains of Trichoderma reesei endoglucanase I, II,
and III, expressed in Escherichia coli , H. Nakazawa, K. Okada, R. Kobayashi, T.
Kubota, T. Onodera, N. Ochiai, N. Omata, W. Ogasawara, H. Okada and Y.
Morikawa*, Appl. Microbiol. Biotechnol., 81, 681-9, (2008)66) The essential acidic amino acid residues for catalytic activity of an exo-beta-dglucosaminidase from Trichoderma reesei, M. Ike, W. Ogasawara, H. Okada and Y.
Morikawa*, J. Mol. Catal. B: Enzymatic., 55, 55-60, (2008)67) Identification of the cis-acting elements involved in regulation of xylanase III gene
expression in Trichoderma reesei PC-3-7, T. Furukawa, Y. Shida, N. Kitagami, Y.
Ota, M. Adachi, S. Nakagawa, R. Shimada, M. Kato, T. Kobayashi, H. Okada, W.
Ogasawara and Y. Morikawa*, Fungal. Genet. Biol., 45, 1094-102, (2008)

68) Functional analysis of the egl3 upstream region in filamentous fungus Trichoderma

reesei, Y. Shida, T. Furukawa, W. Ogasawara, M. Kato, T. Kobayashi, H. Okada andY. Morikawa*, Appl. Microbiol. Biotechnol., 78, 515-24, (2008)

2007（H19）
69) Cellobiohydrolase I (Cel7A) from Trichoderma reesei has chitosanase activity, M.
Ike, Y. Ko, K. Yokoyama, J. Sumitani, T. Kawaguchi, W. Ogasawara, H. Okada andY. Morikawa*, Mol. Cat. B: Enzyatic., 47, 159-163, (2007)

2006（H18）70) Cloning and heterologous expression of the exo-beta-D-glucosaminidase-encoding
gene (gls93) from a filamentous fungus, Trichoderma reesei PC-3-7, M. Ike, K.
Isami, Y. Tanabe, M. Nogawa, W. Ogasawara, H. Okada and Y. Morikawa*, Appl.

Microbiol. Biotechnol., 72, 687-695, (2006)
71) Cloning, functional expression and promoter analysis of xylanase III fromTrichoderma reesei, W. Ogasawara, Y. Shida, T. Furukawa, R. Shimada, S.
Nakagawa, M. Kawamura, T. Yagyu, A. Kosuge, J. Xu, M. Nogawa, H. Okada and Y.
Morikawa*, Appl. Microbiol. Biotechnol., 72, 995-1003, (2006)

72) Purification, characterization, and gene cloning of 46 kDa chitinase (Chi46) fromTrichoderma reesei PC-3-7 and its expression in Escherichia coli, M. Ike, K.
Nagamatsu, A. Shioya, M. Nogawa, W. Ogasawara, H. Okada and Y. Morikawa*,

Appl. Microbiol. Biotechnol., 71, 294-303, (2006)

2005（H17）73) Isoforms of dipeptidyl aminopeptidase IV from Pseudomonas sp. WO24: role of thesignal sequence and overexpression in Escerichia coli, W. Ogasawara, C. Tanaka, M.
Suzuki, G. Kobayashi, Y. Ogawa, H. Okada and Y. Morikawa*, Protein Expr. Purif.,241-251, (2005)

2003（H15）74) Sugar-anionic clay composite materials: intercalation of pentoses in layered doublehydroxide, S. Aizawa, H. Hirahara, K. Ishiyama, W. Ogasawara, Y. Umetsu and E.
Narita*, J. Solid State Chem., 174, 342-348, (2003)

2001（H13）75) Direct intercalation of amino acids into layered double hydroxides bycoprecipitation, S. Aizawa, S. Takahashi, W. Ogasawara, Y. Umetsu and E. Narita*,

J. Solid State Chem., 162, 52-62, (2001)

2000（H12）76) Template mineralization of ordered macroporus chitin-silica composites using a
cuttlebone-derived organic matrix, W. Ogasawara, W. Shenton, S. A. Davis and S.
Mann*, Chem. Mater., 12, 2835-2837, (2000)

77) Corprecipitation behavior of amino acids with Zn-Al layered double hydroxide
precipitates, S. Aizawa, S. Takahashi, W. Ogasawara, Y. Umetsu and E. Narita*,

Clay Science, 11(3), 17-328, (2000)

1999（H11）78) Uptake of anionic dye by layered double hydroxide dispersed to siliceous porous
substances, T. Yamagishi, W. Ogasawara and E. Narita*, Clay Science, 11, 1-10,(1999)

1997（H9）79) Purification and chracterization of a Dipeptidyl carboxypeptidase from

Pseudomonas sp. WO24, W. Ogasawara, N. Abe, T. Hagio, H. Okada and Y.
Morikawa*, Biosci. Biotechnol. Biochem., 61, 858-863, (1997)

80) Purification and characterization of Dipeptidyl Aminopeptidase from

Aureobacterium sp. WO26, W. Ogasawara, N. Inanobe, K. Ochiai, K. Ando, H.
Okada and Y. Morikawa*, Biosci. Biotechnol. Biochem., 61, 146-151, (1997)

1996（H8）81) The gene encoding Dipeptidyl Aminopeptidase BI from Pseudomonas sp. WO24:
cloning, sequence and expression in Escherichia coli, W. Ogasawara, G. Kobayashi,
S. Ishimaru, H. Okada and Y. Morikawa*, Gene, 206, 229-236, (1996)

82) Dipeptidyl Aminopeptidase IV from Pseudomonas sp. WO24, W. Ogasawara, Y.
Ogawa, K. Yano, H. Okada and Y. Morikawa*, Biosci. Biotechnol. Biochem., 60,
2032-2037, (1996)

83) Two types of novel dipeptidyl aminopeptidases from Pseudomonas sp. WO24, W.
Ogasawara, G. Kobayashi, H. Okada and Y. Morikawa*, J. Bacteriol., 128, 62886295, (1996)84) A novel dipeptidyl aminopeptidase from Pseudomonas sp. WO24, W. Ogasawara, K.
Ochiai, K. Ando, K. Yano, M. Yamasaki, H. Okada and Y. Morikawa*, J. Bacteriol.,
178, 1283-1288, (1996)