PERSONAL PRECISIONUSER GUIDEWELCOME
Thank you for choosing the MyGo Mini.
The MyGo Mini is the world’s most compact
real-time PCR instrument, with no moving parts
providing silent operation and maximum reliability.
With outstanding precision, it is capable of providing
great results over a broad range of applications.
Personal precision...

2

CONTENT
This quick start guide will teach you what you needto know to start running your MyGo Mini. It describes
everything from opening and closing the lid, to data
analysis for your qPCR experiments.
Contents include...

SETUP

A N A LYS I S

Learn how to install your
MyGo Mini - from setting it
up on your workbench, toconfiguring the instrument
and installing the software.

Learn how to easily analyse
your results.

E X P O RT
Learn how to export your datafor use with other software.

TIPS
Learn some useful tips forusing your instrument.

MAINTENANCE
Learn how to look after your
MyGo Mini.

EXPERIMENT
Learn the basics of setting
up an experiment on your
MyGo Mini.

TROUBLESHOOTING
If you ever have a problem
with your instrument, learn
how to troubleshoot it here.

3

SETUPI N S TA L L I N G YO U R M YG O M I N I
You should have the following items in the MyGo Mini box:

1. MYGO MINI QPCR INSTRUMENT
2 . M YG O M I N I U S E R G U I D E
3 . POWER SUPPLY UNIT AND MAINS CABLE
4. ETHERNET CABLE
5. USB DRIVE
6 . U S B D R I V E EXTENSION CABLE
Please keep the box the MyGo Mini came i​n, in​case you need toprotect your MyGo Mini during storage or shipment in future.

Lid

Heatsink

Display LEDs
Foot
4

LID
The lid of the MyGo Mini
can be removed from the
instrument​and must be
attached at all times when
not in use. ​To remove thelid push down and turn
anticlockwise. ​To attach
the lid, first line up the lugs
on the lid with the grooves
on the mount plate as
shown below. Once aligned
press down, and then turnclockwise to secure until you
feel a hard stop.

If the instrument isconnected to power the
instrument will say “Lid on”
and “Lid off” when you ​attach​and remove the heated lid.
The instrument will display
green when the lid is fitted,
and display pulsating yellow
when the lid is removed.
The lid may get warm during
operation;​this is normal.

Lugs

Grooves

H E AT S I N KThis is the outer body ofthe instrument and enables
efficient cooling of thesystem during thermal ​cycling. Ensure​that there
is free airflow around the
instrument - do not coveror wrap the instrument,
and keep it at least 5 cm
away from objects or walls.

F O OT
The foot should always beplaced facing down on yourworktop during operation.
5

M YG O M I N I CO N N EC T I O N S
If you turn your MyGo Mini upside down you will see
three connections as shown below:

POWER
This is to connect your MyGo Mini to the provided power supply unit.

USB Port
Ethernet PortPowerU S B P O RT

E T H E R N E T P O RT
This is for connecting your
MyGo Mini to your LAN or
computer.

Insert the USB drive here
to run an experiment fromthe USB drive, or to update
firmware using a USB drive. ​You can use the supplied USB
drive extension cable.
6

POWERING ON YOUR MYGO MINI
Connect the power supply to the MyGo Mini, place
the MyGo Mini on your lab bench, and then connect
AC power to the power supply unit. Your instrument
will now turn on after a few seconds. The display LEDs
will light up multi-coloured, and then turn green if a lid
is attached, or yellow if not. ​Your MyGo Mini is readyto run.

CO N N EC T I N G
YO U R M YG O M I N I
TO A N E T W O R K ,P C O R L A P TO P
Connect one end of
the ethernet cable tothe instrument and theother end to a LAN port,
or directly to your computer. ​Once your MyGo Mini isconnected to a LAN, you canconnect to your instrument via
Wi-Fi if your network supports it.
A summary of these connections
is shown on the following page...
7

MyGo Mini Connection Modes

1. Direct network connection

2. Local area network connection

3. USB drive connection

8

MYGO MINI LIGHTS AND SOUNDSThe MyGo Mini uses display LEDs and sounds tohelp you understand what the instrument is doing.
Here is a summary of these display LEDs and sounds:
D I S P L AY L E D s
GreenIdle / Ready to run

Yellow

Lid off

Blue

Experiment is starting/cooling down

Blue

Running, low temperature

Pink

Running, medium temperature

Red

Running, high temperature

Multi-Coloured

Experiment complete

Red-Flashing

Fault detected

SOUNDS“Lid On”

The lid has been attached“Lid Off”

The lid has been removed“Analysis Starting”

An experiment has begun“Analysis Complete”

An experiment has been
completed9I N S TA L L I N G YO U R
M YG O M I N I S O F T WA R EYour MyGo USB drive contains software for Windows,
Mac OS X and Linux operating systems. Please open
the software file matching your chosen operating
system. The latest version of the software can also be
downloaded from our website mygopcr.com.

WINDOWS

M AC O S X

Double-click​ ​on thewindows installer and follow
the on-screen instructions
to install your MyGo Mini
software on Windows.

Double-click​on thedisk image and follow
the on-screen instructions
to install your MyGo Mini
software on your Mac PC.

10

CONFIGURING YOUR MYGO MINI
With your MyGo Mini software open and your
MyGo Mini connected to the network please connect
to your MyGo Mini Instrument.

Please select Configuration

11

Please select MyGo Mini

To add your new MyGo Mini
select the Add b​utton. You
will now be presented witha list of available instruments.

12

Double-click​on the
instrument you wish toconnect ​to, or​ pr​ess ​Select ​with the instrument selected.
The instrument will now
be added to the list of ​Registered Instruments
for use in the software.

13

TIPS
Here are some great tips to keep in mind whilst running your
MyGo instrument.

1. LID GETS WARM
2 . D O N OT L E AV E T H E L I D O F F
3 . SPIN YOUR TUBES
4 . R E M OV E A L L B U B B L E S
5 . K E E P YO U R L A B C L E A N
6 . K E E P YO U R I N S T R U M E N T C L E A N
7. PC SETTINGSL I D G E T S WA R M
The MyGo Mini lid can get warm during operation, this iscompletely normal.

D O N OT L E AV E T H E L I D O F F
If the lid is off dust may fall into the wells and affect theperformance of your instrument.

S P I N YO U R T U B E SThis should ensure that all contents are at the bottom of thetubes, and there are no bubbles present. Reaction mixtures
which are viscous, or contain high levels of detergents, will
require stronger centrifugation to remove bubbles. Please use
sufficient g-force to ensure that no bubbles are present.
14

R E M OV E A L L B U B B L E S
Bubbles can cause optical artefacts as shown in the graphbelow. Ensure that no bubbles are present in reaction volumes.

Intensity (RFU)

ArtefactCycle

K E E P YO U R L A B C L E A N
Please keep your work space clean including all lab equipment
like surfaces, pipettes, and tube racks. This will keep the
instrument clean and help maintain good results.

K E E P YO U R I N S T R U M E N T C L E A N
We recommend a routine cleaning of your instrument. To do so
follow the cleaning guide in the Maintenance section.

PC SETTINGS
Please disable all power saving settings e.g. sleep andhibernate.
15

EXPERIMENTThis section will teach you everything you need to know
to get started with MyGo Mini experiments. You willlearn how to create, save, open and close experiments.
You will​also learn how to set up an experiment
including thermal profile, sample information and opticalsettings. Finally we will show you how to run your new
experiment from the software and from the USB​drive ​provided.

CREATE A NEW EXPERIMENT
Below is the MyGo Mini home screen.

Click New to create
a new experiment.

16

E X P E R I M E N T S U M M A RY
Once you have created a new experiment theE​xperiment Summary w​ill be displayed as shown below:

By default the experimentname will be “N​ew
Experiment” ​with a date
and time stamp, which
can be edited.
The summary will give
you information about the
instrument you are running,
the settings chosen and any
useful notes you choose to
add about your experiment.

17

OPEN AN EXPERIMENT
By selecting O​pen y​ou can open an experiment asshown below:

FILES OF TYPE
The ​Files of Type drop downmenu gives you the option to
open an experiment normallyor as a template that can beused for a new run.

18

EXPERIMENT FILE
An experiment file will containall the data and analyses but
can not be re-run.
To open as an Experiment
File s​elect the first option
shown on the opposite page.

EXPERIMENT FILEA S T E M P L AT E
Opening an experiment file
as a template allows you to
use the same profile, samples,
settings and analyses to
re-run a previous experiment,
generating new data. When an
experiment file is opened asa template, it has the same:
1. Temperature profile.
2. Optical settings.
3. Samples and targets.
4. Analyses, including settings.
If you wish to open anE​xperiment File as Templates​elect the second option.

USB RUNTo open a USB run navigate to the USB location using the Opendialog window shown to the left. Select Experiment File in theFiles of Type drop down menu and then double click on the USB
run.
19

SAVING AN EXPERIMENT
Once an experiment has completed it can either be
automatically saved to a pre-set location on yourcomputer or manually saved by you. The experimentmust be saved before the software is closed to ensurethat data is not lost.
AU TO M AT I C
SAV I N G

To automatically save yourdata when the experimenthas finished, firstly click
Browse and choose thelocation to save your run to.

When starting a run you will
be prompted with the window
displayed below.

Make sure the first checkbox is selected and yourexperiments will be automatically saved. To preventthe software from showing this dialog again, select thesecond checkbox. You can change these settings laterby clicking Configuration and selecting General Config.

20

M A N UA L SAV I N G
Once your experiment has finished you can save your experimentmanually by selecting S​ave or S​ave As. I​f your experiment isunsaved, you will be prompted to choose a location to save yourexperiment. If your experiment is already saved you can save anychanges made to the experiment by selecting S​ave, ​which will
overwrite the previous version.

C LO S I N G A N E X P E R I M E N T
To close an experiment select C​lose. ​If the experiment has notbeen saved the following dialog box will appear:

21

SETTING UP A PROFILE
In this section we will cover Hold Times, Programs andsetting up a temperature profile.
HOLD TIMES
During thermal cycling protocols hold times should be chosen
to allow for the following: thermal equilibration of reaction
volumes; completion of biochemical reactions, for example fulllength strand extension; and optical data acquisition.
Typically, optical data is acquired at the same temperaturethat strand extension occurs. By default this time is set to 30
seconds, However, this time can be reduced to a minimum of
3 seconds, to save time, or increased to more than 30 seconds
in order to increase PCR efficiency and optical sensitivity.
For other hold times during cycling protocols, by default times
are set to 10 seconds. However, this time can be reduced to aminimum of 3 seconds, to save time, or increased to more than
10 seconds in order to increase PCR efficiency.

22

A thermal profile is required to perform an experiment.

Select the profile tab as shown below:

PROGRAMS
To add a program to your experiment clickA​dd i​n the P​rograms p​ane. Programs canbe deleted by selecting them in the list thenclicking D​elete. ​The program order can also
be altered by selecting the program to movethen clicking the U​p a​nd D​own arrows.

23

T E M P E R AT U R E P R O F I L E
Once programs have been added to the experiment they willappear in the T​emperature Profile p​ane in the order they arepresent in the P​rograms ​window as shown below:

PROGRAMSETTINGS
To alter the settings ofa particular program you
should first select it, thenrefer to the right hand ​pane.
In​the example above the ​Melting program is selected
and can be edited in theright hand pane.

24

SAMPLES
You will now learn how to set up samples and targets
which can be defined as follows:
SA M P L E S

TA R G E T S

A description of the specimen
being analysed e.g. “Mouse#5”

A molecular target e.g. “x174”,detected with a specified
fluorescent reporter e.g. “FAM”

Samples or targets do not need to be defined
before starting a run. They can be added toan experiment, whilst it is running, or once it
has finished. With a new experiment created,
please select the ​Samples tab as shown below:

25

A D D I N G SA M P L E S A N D TA R G E T S
Samples and targets can be added and removed fromthe experiment by selecting + and - in the ​Samples
and Targets pane.
The order of samples and targets can be alteredby clicking the ​Up and D​ own arrows in the ​Samples
and Targets pane.

A S S I G N I N G SA M P L E S TO W E L L S
Assign wells by selecting them and clicking ​Set.
Samples can also be unassigned by selecting
wells you wish to un-assign and clicking ​Clear.

A S S I G N I N G TA R G E T S TO W E L L S
Targets can be assigned to wells as a Standard,
Unknown or Negative by clicking Std., Unk., or Neg.,
respectively. Assignments can be cleared by clicking
Clear with the targets and wells you wish to clear
selected. For more information about different
types of standard controls please contact us on
support@mygopcr.com.

26

DY E CO M PAT I B I L I T Y
The MyGo Mini comes pre-calibrated for 11 different fluorescentlabels, shown in the table below. The Auto Dye File Generation
software analysis module enables you to calibrate your MyGo Mini for
additional fluorophores if required.

Factory Calibrated Dyes 		
		
		
		
		

27

ATTO 490LS, CAL 540,
CAL 560, FAM, HEX,LC Green, ResoLight,
ROX, SYBR Green,
SYTO 9, VIC

SAV I N G A N D O P E N I N G A SA M P L E S E T U P
If you wish to save or open sample and target information setup
you can select ​Save As... or Open, respectively.

Sample and target setups can be saved in the following formats:

CSV

Editable .csv files.

PSD

A proprietary locked file format.

RDML

The Real-time PCR Data Markup Language (RDML)
is a structured and universal data standard forexchanging quantitative PCR (qPCR) data.

28

RUN SETTINGS
The MyGo Mini software provides
default optical settings which do
not need to be changed for mostapplications. To view them, selectthe R​un Settings tab.
C YC L I N G ACQ U I S I T I O N S
During cycling, by default, optical data
will be acquired with settings compatible
with a broad range of assays.

M E LT ACQ U I S I T I O N S
During melting analysis, optical data
will be recorded every 500ms. Thisrate provides relatively compact
experimental data file sizes, andsupports demanding High
Resolution Melting assays.

If you need greater control of how
optical data is acquired during
your experiment, select “UseAdvanced Settings”.

29

A DVA N C E D S E T T I N G S
Here, if necessary, you can tailor optical settings toyour assay’s characteristics.
Integration Time controls the exposure time, in seconds, of the
optical sensor inside the MyGo instrument. Brighter reagentsrequire shorter integration times than dimmer reagents.
Longer integration times will provide improved signal to noisewith dim fluorescent reporters. Short integration times will
reduce the risk of the fluorescence detection system being
saturated by very bright fluorescent reporters.
C YC L I N G ACQ U I S I T I O N S - I N T EG R AT I O N T I M E ( S )Here you can specify the integration time (in seconds) for
optical data acquisitions made during thermal cycling.
M E LT ACQ U I S I T I O N S - I N T EG R AT I O N T I M E F O R M E LT ( S )Here you can specify the integration time (in seconds) for
optical data acquisitions made during melting analysis.

30

DY E C A L I B R AT I O N S
Here is where you can review the current dyes present
in your experiment. You have the following options
when working with dyes in your MyGo Mini software:
OPEN

Open dye calibrations from a file to be added tothis experiment.
SAV E

Save a dye calibration from your experiment to a file on yourcomputer. These can then be used on multiple computers and
MyGo Mini instruments.
R E M OV E

Remove dye calibrations from this experiment. Factory
calibrated dye files can not be removed.

31

STARTING A RUN FROMTHE SOFTWARE
To start a run from the MyGo Mini software selectS​tart Run. Y​ou will then be presented with the autosave options (unless you have chosen not to beprompted) and then be asked to choose an instrumentfrom the list of R​egistered Instruments. ​Select an
instrument and press S​elect t​o begin the run.

STARTING A RUN FROM
A USB STICK
To start a run using a USB drive select ​Start Run. Then,
when you are asked to choose an instrument, select ​Start run from USB. You will then be prompted to findthe location of your USB drive. Once selected press ​Save and safely remove your USB drive as normal. The
USB drive can now be placed into the instrument andyour experiment will start automatically.

32

LABELLING YOUR TUBES
You can write on the lids of your tubes. Please do notwrite on the sides of your tubes. Ink can transfer fromthe sides of tubes to the walls of the wells, and this canaffect the performance of your instrument.

LOADING TUBES INTO
YOUR MYGO MINIIn order to ensure that the heated lid is balanced, please
make sure that the mount contains a tube in each of
positions 1, 5, 9, and 13. These positions can be filledwith tubes containing reagents, or empty tubes.
5

1

9

13
33

ANALYSISThis section will teach you about different typesof analysis available. These can be grouped into thefollowing groups. For information about more analysis
options please contact technical support.
Q UA N T I F I C AT I O NDetermine accurate quantities
of template or relative expression
levels of genes using Absolute
Quantification ​or R​elative
Quantification. ​G E N OT Y P I N G
Use TaqMan probes or High
Resolution Melting to perform
SNP genotyping using A​utomatic
Endpoint Genotyping ​orA​utomatic High Resolution Melt. ​M E LT I N GAutomatically determine themelting temperature of youramplicons using A​utomatic
Tm Calling.

RUN FUSION
Combine data from multiple
runs on your MyGo Mini foranalysis together.
34

SELECT ANALYSIS TYPE
You can add an analysis type by selecting the “+”button in the bottom left of the software window.
Analysis types are separat​ed int​o ​Automatic and M​ anualsections. ​Automatic analysis types use ​advanced automateddata processing to provide accurate results with minimal userintervention. M​ anual analysis types enable users to apply
traditional analytical methods.

Ticks and crosses indicate
which analyses can be applied
to the data in your experiment.

35

AU TO M AT I C Q UA N T I F I C AT I O N
P O S I T I V E / N EG AT I V E C A L L I N G
The first step in amplification analysis is to determine whichtargets have produced a positive amplification curve, and which
amplifications have produced a negative amplification curve.
For every amplification curve the software calculates
three metrics, these metrics are used to determine which
amplifications are positive. These metrics are:
Q UA L I T Y

Quality is a measure of how clean an amplification appears.
This value will tend to be higher for amplifications with a clear
exponential phase and steady baseline, and lower for noisier orflatter curves.

High
Low

36

INTENSITY

Intensity is a measure of the size of the exponential phase of theamplification. Note that this may be smaller for amplifications
with a negative baseline drift.

High
Low

PERFORMANCE

Performance is a measure related to the observed efficiency of
amplification at later cycles. This value will tend to be lower for
inhibited or out-competed reactions, and higher for efficient reactions.

High

Low

37

SETTINGS
The software uses a combination of Quality (Q), Performance(P), and Intensity (I) thresholds to determine if an amplificationis positive (green zone) or negative (red zone). All values (Q, P
and I) must be above the relevant threshold for an amplification
to be called positive. The Settings tab enables you to review
metrics for amplifications and set appropriate thresholds.

The dots represent individual amplification curves in yourexperiment. All dots that are within the top right of thegraph are called positive and will be subject to normalization
determined in Advanced Settings.
To switch between “Intensity vs Quality” and “Performancevs Quality” select the Graph Axes drop down menu. All 3
thresholds are used for calling even if they are not currently
displayed on the graph.

38

A RT E FAC T F I LT E R I N GBiochemical and physical factors can cause fluorescence levels
to change during a run. These are often observed during early
cycles. Filtering out these artefacts can improve the accuracyof amplification analysis. Two settings enable you to control theprocess of filtering out such artefacts.
E XC L . C YC . M I N .

The software will exclude at least this number of cycles fromdata before performing analysis.
AU TO E XC L . C YC .

By default the software will automatically determine which
cycles at the beginning of a run exhibit artefacts, and exclude
this number of cycles from data before performing analysis.
This feature can be disabled by deselecting this option.

39

N O R M A L I SAT I O N O P T I O N S
Here you can choose the way your data is normalised before
display in the amplification graph. By default Full normalisation
is applied to enable the discrimination of subtle differences inamplification. Alternative modes are provided to assist datavisualisation when troubleshooting challenging assays.
You can choose between the following modes:
Normalisation Mode
None

Background

Full

Background Correction
Normalisation

BAC KG R O U N D CO R R EC T I O N

Background fluorescence, including drift, is estimated andremoved. There are many potential sources of background
fluorescence, e.g. emissions from unquenched probes.

N O R M A L I SAT I O N

Positive amplifications are rescaled to account for intensity
variation between wells. Uncorrected intensity can beaffected by a number of factors, e.g. the optical properties ofdisposables. Normalised amplifications are rescaled to a nominal
value of 1 relative fluorescent unit, with exact endpoint values afunction of curve shape.

40

R E S U LT S A S TA B L E
The results of amplification analysis are presented in the Results as
Table view.

Q UA N T I F I E R S
If you have assigned standards with different known quantities
to your experiment, for example a dilution series, you will be able
to see your reaction efficiency by selecting Quantifiers. Here you
will be shown the relationship between Cq and input template
quantity, plus a variety of statistics derived from the standards.

R E L AT I V E Q UA N T I F I C AT I O N
If you are performing relative quantification analysis then selectR​elative Quantification a​t the top of the A​uto Quant. pane.

41

M U LT I P L E X I N G
DY E PA I R S
The MyGo Mini supports multiplexing with the following dye
pairs.
Dye Pair

Notes

FAM, HEX

Cost effective probe synthesis

FAM, VIC

e.g. Applied Biosystems
TaqMan SNP Genotyping
Assays

FAM, CAL560
FAM, CAL540
FAM, ATTO490LS

Recommended for challenging
applications

F O R B E S T R E S U LT S :•Ensure that there is no competition between the different
PCR in the multiplex reaction.
•Ensure that probes have low background fluorescence, forexample by using dual-quenched probes.
•Ensure that probe signal strength is balanced, by using anappropriate concentration of primers and probes.
•Make sure to select the correct dye for each Target.
•Generate assay specific dye files if necessary.

42

TIPS
Spectral properties of fluorophores can vary as a result ofmany factors, including stereochemistry, sequence context
and buffer chemistry. If you observe spectral crosstalk,generate assay specific dye files to compensate forthese effects.

DY E F I L E G E N E R AT I O N
DY E E X P E R I M E N T
Dye file generation allows you to calibrate your MyGo Mini for
new dyes that are not included in the factory-calibrated list. Thiscalibration is based on a PCR amplification using the relevant
dye or labelled probe.

DY E C A L I B R AT I O N R U N
You will need to perform a real time PCR run in order togenerate a dye file. Every well of your MyGo Mini should containthe same reagents. These reagents should produce a single PCR
product, and a strong signal for the dye that you are calibrating.
Create a sample named after the dye that you are calibrating,and assign that sample to all wells.

43

AU TO DY E C A L I B R AT I O N
Select the Auto Dye Calibration option in the Select Analysis
Type window. The Auto Dye Calibration analysis module will
analyse changes in fluorescence during the dye calibration run
to create a new dye file.

DY E DATA

Select this tab to view the wells that will be used in thecalibration.
A M P L I F I C AT I O N

Select this tab to view the cycles that will be used in thecalibration.

44

E X P O RT C A L I B R AT I O N
Select the Dye Data tab and you can choose to export thecalibration to a file, or, use it in your current experiment.

Select the Use Wells drop
down menu and you will see
the list of samples present in
the experiment. Select the
sample you wish to create adye from.

U S E C A L I B R AT I O N I N C U R R E N T E X P E R I M E T

By selecting this option your new dye file will appear in thetarget list in this experiment. This option will not save the dyefile to your computer.
E X P O RT C A L I B R AT I O N TO F I L E

Alternatively you can save this new dye file to your Mac or PC.

45

G E N OT Y P I N G
AU TO M AT I C E N D P O I N T G E N OT Y P I N G
TaqMan genotyping experiments can be analysed automaticallyusing A​utomatic Endpoint Genotyping. ​Genotypes of samples
are determined by the ratio of endpoint fluorescence between
two TaqMan​​probes. Thresholds for the fluorescence ratios are
generated automatically as shown below:

AUTOMATIC HIGH RESOLUTION MELTING
A​utomatic HRM c​an be performed by using theA​utomatic HRM m​odule as shown on the following page:46

D I F F E R E N C E , N O R M A L I Z E D A N D M E LT

Once opened the analysis module will automatically determine
optimised parameters and generate a set of D​ifference,
Normalized a​nd M​elt g​raphs.
C LU S T E R I N G

The difference curves will then be converted into a clusteringgraph showing clusters of different genotypes.

G E N OT Y P E S

The software will automatically determine I​nferredgenotypes b​ased on the clustering graph. You can define yourcontrol G​enotypes u​sing the G​enotypes p​ane.

47

M E LT I N G
AU TO M AT I C T M C A L L I N G
Below an​​Automatic Tm Calling ​module has been added to theexperiment and the software has automatically identified andcharacterised a melting peak.

Tm values will
be presented
in the table48

AU TO S E T T I N G S
The user has the option
to change the target if
the experiment contains
more than one target. The
signal-to-noise ratio setting
determines how big a peakshould be before it is called.
Increasing this value will
increase specificity ofpeak detection. Decreasing
this value will increase thesensitivity of peak detection.

P E A K S A N D M E LT S
The P​eaks p​ane will show
you the melt peaks withmelting point indicators.
The M​elts p​ane will show
you the melting curvesof the same reactions.

49

RUN FUSIONRun Fusion enables you to combine data from multiple
runs from your MyGo Mini together for analysis. To fusemultiple experiments together hold down ​cmd on a Mac
or Ctrl on a PC and select the files you wish to fuse fromthe open dialog window as shown below.

Select ​Open and then from thenext dialog select ​Fuse 2 files
into one. You can also open
files as separate experiments
using ​Open 2 Files.

50

If you are fusing experiments from multiple file locations you
must select the first experiment and then select the “+” from the
top left of the open file dialogue window as shown below.

Now hold cmd/Ctrl andselect the next experiment.
Select ​Open to fuse yourexperiments together.

51

EXPORT
When you have finished analysing your data in the
MyGo software, you can export the results in a variety
of ways, from raw data to user defined custom
reports. This section will take you through the steps
you need in order to do this.

TABLE EXPORTFrom panes showing
results in tabular format
you can export data using​the ​Export button.

52

To export data click the Export button, shownon the previous page. Data can be exported in thefollowing formats:
CSVThis is an editable data file that can be opened in many
spreadsheet applications.

PDF

This file format is suitable for ​archiving, printing​andpresentations, but cannot be edited.

RDML EXPORTThe software is able to export Cq values andamplification curves from A​uto Quantification andmelt curves from A​utomatic Tm Calling i​n RDML
format. T​​o do this select S​ave as, ​as if you were saving
your experiment. Now from the F​iles of Typed​rop down menu, select Real-Time PCR Data Markup
Language a​s shown below:

53GRAPH EXPORTFrom panes showing results as graphs, such as
amplification and melt curve data can be exported
by selecting the button from the below example.

The user can export information as bitmap images (.PNG),
Scalable Vector Graphics (.SVG), Character-Separated Variable(.CSV), or Portable Document Format (.PDF).

PNG

This is a bitmap image.

SVG

A Scalable Vector Graphic is an XML-vector based graphicthat can be used where a high resolution scalable graphis required, for example for papers or web design.

CSV

This contains information about the graph you areexporting in spreadsheet format.

PDF

This contains the graph you are exporting in the Portable
Document Format. The graph is in vector format forhigher quality.
54

R E P O RT G E N E R AT I O N
Customisable reports can be generated from your experiment
by selecting ​Experiment Report under the ​Experiment tab. ​This
provides you with the ability to choose ​which parts of the data ​toinclude​in a report. ​For example, ​items such as thermal profiles,
sample information and various analysis results, e.g. ​Tm ​values orquantities can be ​included, or excluded from reports.

Please select Experiment Report55

MAINTENANCE
This section will help you take care of your MyGo Mini. ​It covers ​the following​areas:
1. CLEANING

3 . ENVIRONMENTAL
CO N D I T I O N S

2. D I S P O SA B L E S

4 . D I SA S S E M B LY

56

CLEANING
DAY-TO - DAY
For day-to-day cleaning wipe the external surface ofyour instrument with a damp, soft, lint-free cloth. Then
dry your instrument with another soft, lint-free cloth.
Notes: Avoid abrasive cloths, towels, paper towels, and similar
items that might cause damage. Before cleaning your instrument
unplug all external power sources, devices, and cables. Don’t get
moisture into any openings.

D ECO N TA M I N AT I O N
If your instrument needs decontaminating please
follow the instructions contained in the MyGo
Decontamination Guide​.​Please note that for health and safety reasons you must print andcomplete a physical copy of the decontamination form, and include
this with any instrument or lid that is returned. The form must be sentwith the instrument, and we can not accept electronic versions of theform. Failure to comply with these guidelines will result in a charge fordecontamination.

DEEP CLEANING WELLS
Automated background subtraction processes meanthat low levels of fluorescence contamination in
the wells of your instrument will not affect system
performance. Should one or more wells of yourinstrument become contaminated with high levelsof fluorescent substances, and need cleaning, pleasecontact technical support for guidance.
57

DISPOSABLES
We recommend the use of MyGo disposables
for optimal results. If you wish to use third party
disposables please note the following:
A . Physical dimensions should be the same as the 		
MyGo disposables to ensure that they fit into yourinstrument, without damaging it.
B. Caps should seal tubes effectively in order to prevent
variability caused by evaporation, and to minimise
the risk of contamination with PCR products.
C . Disposables should have reproducible wall 		
thicknesses, which are thin enough to ensure rapid
temperature equilibration, but thick enough to 		
avoid breakages.
In many cases third party disposables will have inferior thermaland optical characteristics, which will reduce the quality of
results obtained from the system.

ENVIRONMENTAL CONDITIONSYO U R W O R K S PAC E
Your MyGo ​Mini​instrument should be placed on a ​flat​, dry, ​surface​that is not subject to draughts.​Do not install your
MyGo ​Mini​instrument directly in the flow of air from an air
conditioner or fan.​Do not install your MyGo Mini instrument
in a dusty environment.
58

P R E V E N T I N G CO N TA M I N AT I O NPrevent contamination by wearing gloves, using clean
tube racks, and filter tips. Make sure tubes are sealed, PCR
product is disposed of, and leaks are cleaned immediately.

E N V I R O N M E N TA LO P E R AT I N G CO N D I T I O N S
Humidity

MAX: 80% at +32°C
MIN: 30% at +15 to +32°C

Temperature+15°C to +32°C

Pressure

0 to 2000 MAMSL
80 to 106Kpa

E N V I R O N M E N TA L S TO R I N G /T R A N S P O RT I N G / PACKING CONDITIONS
Humidity

Temperature

10% to 95%
No condensation-20°C to +60°C

Pressure

0 to 3000 MAMSL
70 to 106Kpa

D I SA S S E M B LY
Please note that your MyGo Mini contains no ​userserviceable​components inside. Any disassembly of
your MyGo Mini instrument will void all warranties.
59

TROUBLESHOOTINGThis section will help you troubleshoot your MyGo Mini
if you think something is wrong.

FAQ
Here are some frequently asked questions,and the answers.
How do I create a template?

All experiments can be used
as a template. Click “Open”
and then using the “file type”
selection in the file dialog toselect the “Template” option,then select the file to open asa template.

Where is the threshold for
determining Cq values in
Auto Quant?

Modern methods ofdetermining Cq values
are not based on simple
thresholds. Modern methodsof Cq determination are model
based. Auto Quant fits a modelof a PCR amplification to thefluorescence data observed.
This model fit then enables
the estimation of a number ofimportant parameters including
Cq values.
60Why do some of myamplification curves drop
down before rising in Auto
Quant?

Correct background removalin Auto Quant relies oncorrect qualitative calling.
Check that appropriate
thresholds are set so that
positive amplifications arecalled positive.

Do I need to use ROX asa passive reference in my
MyGo instrument?

Some real-time PCR
instruments require
the use of ROX as afluorescent passivereference to correct for
optical artefacts. Generally,
modern instruments do not
require the use of passive
fluorescent references. The
MyGo instruments do not
require the use of ROX as apassive reference.

Is it normal for the lid of my Yes.
instrument to get warm during
use?

61

ERROR MESSAGESYour MyGo Mini will let you know when something iswrong by displaying an error. Most errors are reported
as messages in the Status Bar in the MyGo software.
Some errors are reported by the instrument display LEDs.

Status Bar.

62

If you encounter an error, please make sure that the instrument
is running in a lab with the required environmental conditions,tubes have been loaded correctly, the lid has been fitted correctly,
and all cables are attached correctly and securely. If the error still
occurs, you may need to contact technical support with the details
described below.

E R R O R M E S SAG E S - D I S P L AY E D I N
S TAT U S B A R
Message

ActionInstrument will abort
because of a major error.

Please use the log button to viewthe detailed message, and send
this, plus a copy of the experiment
ppf file, to technical support.

Saturation Warning

Please use the log button
to view the detailed message- this will tell you which wells
saw a saturated reading.
To correct, please reducethe integration time setting,or reduce the quantity/
concentration of dye or probes.

Lid was removed during
run - instrument will abort.

The lid must not be removed
until the run finishes. ​See “MyGo
Mini Lights and Sounds” ​for a
description of how to confirm
the instrument has finished​.
If running using the network,
check for the experimentcompleted message.
63

Instrument timed out
waiting for heated lid.

Please ensure the lid is fitted
correctly, and the ambient
temperature is in the required
range of ​+15ºC to +32ºC​.

Could not start run- ​instrument has
encountered amajor error.​

Please use the log button
to view the detailed message,
and send this, plus a copyof the experiment ppf file,
to technical support.

Could not start run- please remove tubes
from previous run.

Remove the tubes, add newtubes, and start the run again.
Please note that you must
remove the lid and refit it
between runs, so that the
instrument knows you haveremoved any tubes froma previous run.

Could not start run- please insert tubes
and fit the heated lid.

Remove the lid, check tubes
are positioned correctly, andrefit lid, making sure it is fully
rotated into locked position,and lights and sounds confirm
it is fitted.

Could not start run- instrument has an error
in heated lid support

Please report error
to technical support.

64

Could not start run- instrument has anerror in heated lid.

Please report error
to technical support.

Received invalid data (X)Please report error, including
the specific number reported
as X, to technical support.

Instrument encountered anerror, and had to abort run.

Please use the log button toview the detailed message,
and send this, plus a copyof the experiment ppf file,
to technical support.

E R R O R S R E P O RT E D BY
T H E M YG O M I N I D I S P L AY L E D S
Some errors are reported by the instrument display
LEDs flashing red. If your instrument display LEDs areflashing red, please contact technical support.

LOG FILESIn the unlikely event that your MyGo Mini has aproblem, you can produce a log file to send to technicalsupport. For software faults, click the Configuration
button > About tab > click Save Logs to File. For
hardware faults please provide the .PPF file for thefailed run. Sample names etc. can be removed before
saving if necessary. If you cannot send the failed run,please send a run performed with empty tubes.
65

NOTES

66

Permitted use case
The MyGo Mini instruments are to be use for Research Use only (RUO) and not to be used in an in vitro
diagnostic (IVD) setting.

If you are contacting us about a suspected fault wewould also recommend you send us the ppf experimentfile from the run where the problem was observed.
All trademarks are the property of their respective owners.
Design and specifications are subject to change without notice.

67

MyGo Mini
www.mygopcr.com

Version 006 (EN)