Celebrating 25 Years
Antibodies for in vivo Research
Since 1997
bioxcell.com

Antibodies for in vivo
Research Since 1997
For over 25 years, scientists have trusted Bio X Cell as their go-to source
for in vivo functional grade antibodies. This is reflected in over 15,000
peer-reviewed publications citing Bio X Cell products. We understandthis responsibility is of paramount importance and remain committed to
producing antibodies of unparalleled quality and consistency, enabling ourpartners around the globe to accelerate research and discoveries.
‘I am totally satisfied with the service Bio X Cell has given us. When anyone asks mefor CTLA-4 antibodies, I always refer them to you. I look forward to another 20 years.’
James P. Allison
Corecipient of the 2018 Nobel Prize in Physiology or Medicine

PRODUCT CATEGORIESPRODUCT FEATURES• Immune Checkpoint Blocking Antibodies• Exceptional Purity• Mouse Cell Specific Depletion Antibodies• Pathogen Free

• Cytokine Neutralizing Antibodies• Ultra-low Endotoxin Level• Biosimilar Antibodies• Advanced Binding Validation• Recombinant Antibodies• Low Protein Aggregation• Contract Services• Matching Isotype Control Antibodies

Featured Products
Bio X Cell antibodies are formulated for in vivo experiments and used extensively in animal models of human disease. They feature greaterthan 95% purity, ultra-low endotoxin levels, and are preservative, stabilizer, and carrier protein-free. Bio X Cell has developed a wide rangeof antibodies for immuno-oncology, immunology, and neuroscience research. Our products are cited in over 15,000 publications that detaila broad range of applications, including in vivo cell-specific depletion, cytokine neutralization, immune checkpoint blockade, and more.
Antigen

Reactivity Application

Clone

Catalog #Recommended
Isotype Control

CD3ɛmoin vivo T cell depletion, in vitro T cell stimulation/activation, IF, FC

145-2C11BE0001-1

BE0091CD4

mo

in vivo CD4+ T cell depletion, FC

GK1.5

BP0003-1

BP0090CD4mo

in vivo blockade of CD4+ T-cell responses, WB

YTS 177BE0003-3BE0089CD8αmo

in vivo CD8+ T cell depletion

2.43

BP0061BP0090CD8β (Lyt 3.2)

moin vivo CD8+ T cell depletion, in vitro CD8 blockade, IF53-5.8

BE0223BE0088

CD16/CD32moin vivo Fc receptor blocking, in vitro Fc receptor blocking

2.4G2

BE0307BE0090CD40

mo

in vivo CD40 activation, in vitro B cell stimulation/activation

FGK4.5/FGK45

BE0016-2

BE0089BE0091

CD40L (CD154)

moin vivo blocking of CD40/CD40L signaling, in vitro blocking of CD40/CD40L signaling

MR-1

BE0017-1

CD47

hu/mo/rtin vivo CD47 blockade, in vitro CD47 blockade, IFMIAP410

BE0283BE0083

CD71 (TfR1)

moin vivo depletion of CD71+ cells

R17 217.1.3/TIB-219

BE0175

BE0089CSF1R (CD115)

moin vivo macrophage depletion, in vitro CSF-R1 neutralization, in vivo monocyte depletion, FCAFS98

BE0213BE0089CTLA-4 (CD152)

moin vivo CTLA-4 neutralization

9D9BP0164

BP0086CTLA-4 (CD152)

moin vivo CTLA-4 neutralization, in vitro CTLA-4 neutralization

9H10BP0131

BP0087IFNAR-1

mo

in vivo IFNAR-1 blockade, in vitro IFNAR-1 blockade

MAR1-5A3

BE0241

BE0083IFNγ

moin vivo IFNγ neutralization, in vitro IFNγ neutralization, ELISPOT, FC

XMG1.2BE0055

BE0088

IL-10R (CD210)

moin vivo blocking of IL-10/IL-10R signaling, in vitro blocking of IL-10R signaling, FC

1B1.3A

BE0050BE0088

Ly6G

moin vivo neutrophil depletion, in vivo MDSC depletion, IF, IHC-P, IHC-F, FC

1A8

BP0075-1

BP0089Ly6G/Ly6C (Gr-1)

moin vivo depletion of Gr-1+ myeloid cells, FC, IHC-P, IHC-F

RB6-8C5

BE0075BE0090NK1.1

moin vivo NK cell depletion, FC

PK136

BE0036

BE0085BP0089

PD-1 (CD279)

moin vivo blocking of PD-1/PD-L signaling

RMP1-14BP0146

PD-1 (CD279)

moin vivo blocking of PD-1/PD-L signaling, in vitro PD-1 neutralization, IHC-F, FC, WB

29F.1A12

BP0273BP0089PD-L1 (B7-H1)

moin vivo PD-L1 blockade, IF, IHC-F, FC

10F.9G2

BP0101BP0090

TNFαmoin vivo TNFα neutralization, in vitro TNFα neutralization

XT3.11

BE0058BE0088TGF-βhu/mo/rt

in vivo TGFβ neutralization, in vitro TGFβ neutralization

1D11.16.8

BE0057

BE0083VEGFR-2moin vivo blocking of VEGF/VEGFR-2 signaling, in vitro blocking of VEGFR signaling

DC101

BE0060BE0088Biosimilar AntibodiesThe InVivoSIM™ research-grade biosimilar antibodies have the same variable region sequences as the original therapeutic antibodies.
Biosimilars make it possible to study the biological effects of a drug without the need to source an expensive pharmaceutical-grade
therapeutic. They are an excellent choice for use as standard of care/therapeutic benchmarks in functional assays, pharmacokinetic
assays, and in vivo studies in xenograft and humanized mouse models.
Product Name

Application

InVivoSIMTM
Catalog

Recommended
Isotype Control

InVivoSIM™ anti-human C5 (Eculizumab Biosimilar)

Inhibition of the activation of C5, Functional assays, ELISA, IP

SIM0011

BE0349InVivoSIM™ anti-human CD20 (Rituximab Biosimilar)

FC, ELISA, WB

SIM0008

BP0297

InVivoSIM™ anti-human CTLA-4 (Ipilimumab Biosimilar)

CTLA-4 neutralization, FC, ELISA, WB

SIM0004

BP0297

InVivoSIM™ anti-human EGFR (Cetuximab Biosimilar)

GFR blockade, ELISA, FC

SIM0002

BP0297

InVivoSIM™ anti-human HER2 (Trastuzumab Biosimilar)

FC, ELISA, IHC, WB

SIM0005

BP0297

InVivoSIM™ anti-human IL-6R (Tocilizumab Biosimilar)

Functional assays, ELISA

SIM0014BP0297

InVivoSIM™ anti-human IL-17A (Secukinumab Biosimilar)

Functional assays, ELISA, IF, IHC, FC

SIM0013BP0297

InVivoSIM™ anti-human PD-1 (Nivolumab Biosimilar)

Blocking of PD-1/PD-L signaling, FC, IHC, WB

SIM0003

BE0349InVivoSIM™ anti-human PD-1 (Pembrolizumab Biosimilar)

Blocking of PD-1/PD-L signaling, Functional assays

SIM0010BE0349InVivoSIM™ anti-human PD-L1 (Atezolizumab Biosimilar)

FC, WB

SIM0009

BP0297

InVivoSIM™ anti-human TNFα (Adalimumab Biosimilar)TNFα neutralization, FC, ELISA, IF, IP, IHC, WB

SIM0001

BP0297

InVivoSIM™ anti-human TNFα (Infliximab Biosimilar)

TNFα neutralization, FC, ELISA, WB

SIM0006

BP0297

InVivoSIM™ anti-human VEGF (Bevacizumab Biosimilar)

VEGF neutralization, FC, ELISA, IP, WB

SIM0007

BP0297

InVivoSIM™ anti-human VEGFR-2 (Ramucirumab Biosimilar)

Functional assays, IHC, FC

SIM0012BP0297

IF Immunofluorescence | IHC-F Immunohistochemistry (frozen) | IHC-P Immunohistochemistry (paraffin) | WB Western blot | IP Immunoprecipitation | FC Flow cytometry (requires
flurochrome conjugation), Human (Hu), Mouse (Mo), Rat (Rt)

TIME WAITS FOR NO ONE
Enlist the experts at Bio X Cellfor Antibody Production Services
At Bio X Cell, we know that growing hybridoma cells and purifying antibodies
in large quantities with low endotoxin levels can be technically challenging
and time-consuming. Bio X Cell’s optimized fermentation process allows us
to scale up antibody production from milligrams to grams much faster than
traditional methods. Additionally, we offer customizable services including
cell freezing and storage, mycoplasma testing, isotyping, murine pathogen
screening, and more. We know that research moves at a rapid and precise
pace, and we aim to partner with you to achieve your goals faster.
• 25 years of expertise• Fast turnaround time
• Customizable services• Ultra-high purity• Low endotoxin levels• MAP testing

To discuss your antibody production projects,
please reach us at contractservices@bioxcell.com
Phase

Description

1. Pilot

Instructions for preparing and shipping cells.
Assessment of cell line productivity: >30mg of antibody/Liter is required or Bio X Cell cannot proceed to scale up and a pilot fee will be charged ($800 Academic, $1,000 Commercial).

Included✓✓✓✓

Mycoplasma testing service.
Isotyping of cell culture supernatant.
2. Fermentation

Cell culturing and monitoring 7 days/week.

✓✓✓

Freezing of five vials of cells.
Storage of cells at Bio X Cell for rapid resupply.
Expansion of cells using optimized fermentation process.
Concentration of cell supernatant.
3. Purification

Purification of supernatant on Protein A or G.
Buffer exchange to PBS that is preservative and stabilizer.
Expansion of cells using optimized fermentation process.
Sterile filtering of final product.
Testing to confirm <2EU/mg endotoxin. <1EU/mg available for an extra charge.

4. Vialing and QC

Sterile vialing of finished product. Custom aliquoting available for an extra charge.
Internal antibody quality assessment.
Certificate of Analysis
Murine pathogen screening service.

5. Shipping

Shipping on cold packs in environmentally friendly packaging.

Bio X Cell | bioxcell.com | 1.866.787.3444 | customerservice@bioxcell.com

Additional Fee✓✓✓✓✓✓✓✓✓✓✓✓