PD-L1 Antibody / B7-H1 / CD274 [clone PDL1/2746] (V3955)
Catalog No.

Formulation

Size

V3955-100UG

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

100 ug

V3955-20UG

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

20 ug

V3955SAF-100UG

1 mg/ml in 1X PBS; BSA free, sodium azide free

100 ug

Bulk quote request
Species Reactivity Human, Mouse
FormatPurified

Clonality

Monoclonal (mouse origin)

Isotype

Mouse IgG2b, kappa

Clone NamePDL1/2746

PurityProtein G affinity chromatography

UniProt

Q9NZQ7

Localization

Cell surface, cytoplasm

Applications

ELISA : order BSA/sodium azide-free format for coating
Western blot : 1-2ug/ml
Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Limitations

This PD-L1 antibody is available for research use only.

IHC testing of FFPE cervical carcinoma with PD-L1antibody (clone PDL1/2746). HIER: boil tissue sections
in pH 9 10mM Tris with 1mM EDTA for 10-20 min
followed by cooling at RT for 20 min.

IHC testing of FFPE breast carcinoma with PD-L1antibody (clone PDL1/2746). HIER: boil tissue sections
in pH 9 10mM Tris with 1mM EDTA for 10-20 min
followed by cooling at RT for 20 min.

IHC testing of FFPE lung SCC with PD-L1 antibody(clone PDL1/2746). HIER: boil tissue sections in pH 910mM Tris with 1mM EDTA for 10-20 min followed bycooling at RT for 20 min.

IHC testing of FFPE basal cell carcinoma with PD-L1antibody (clone PDL1/2746). HIER: boil tissue sections
in pH 9 10mM Tris with 1mM EDTA for 10-20 min
followed by cooling at RT for 20 min.

Immunofluorescent staining of human Jurkat cells withPD-L1 antibody (clone PDL1/2746) and a CF488 labeledsecondary (green). Nuclei were counterstained withReddot (red).

Western blot testing of mouse and human cell lysateswith PD-L1 antibody (PDL1/2746). Expected molecularweight ~34 kDa (unmodified), 45-70 kDa(glycosylated).

Western blot testing of human cell lysates with PD-L1antibody (PDL1/2746). Expected molecular weight ~34kDa (unmodified), 45-70 kDa (glycosylated).

FACS testing of fixed and permeabilized human Jurkat
cells with PD-L1 antibody; Blue=cells alone,
Red=isotype control, Orange= PD-L1 antibody.

Analysis of HuProt(TM) microarray containing morethan 19,000 full-length human proteins using PD-L1
antibody (clone PDL1/2746). These results
demonstrate the foremost specificity of the PDL1/2746
mAb.
Z- and S- score: The Z-score represents the strength ofa signal that an antibody (in combination with afluorescently-tagged anti-IgG secondary Ab) produces
when binding to a particular protein on the HuProt(TM)array. Z-scores are described in units of standard
deviations (SD's) above the mean value of all signals
generated on that array. If the targets on theHuProt(TM) are arranged in descending order of theZ-score, the S-score is the difference (also in units ofSD's) between the Z-scores. The S-score therefore
represents the relative target specificity of an Ab to its
intended target.

SDS-PAGE analysis of purified, BSA-free PD-L1 antibody(clone PDL1/2746) as confirmation of integrity andpurity.

Description
Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T-cell proliferation, cytokine
production, differentiation of effector T-cells and the induction of BCLX, a promoter of T-cell survival. recruitment of CTLA4
by B7-1 or B7-2, on the other hand, may inhibit proliferation and interleukin-2 (IL-2) production. PD-L1 is 290-amino acidtype I transmembrane protein, which is 20% and 15% identical to B7-1 and B7-2, respectively, has immunoglobulin V-likeand C-like domains and a 30-amino acid cytoplasmic tail. PD-L1 does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS
(inducible co-stimulator). IL-2, although produced in small amounts, is required for the effect of PD-L1 co-stimulation. PD-L2
protein contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region.
Constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the PD-1-PD-L
system could provide unique negative signaling to help prevent autoimmune diseases.

Application Notes
Optimal dilution of the PD-L1 antibody should be determined by the researcher.

ImmunogenA portion of amino acids 39-191 from the human protein was used as the immunogen for this PD-L1 antibody.

Storage
Store the PD-L1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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