J. Inst. Electrostat. Jpn.

, 31, 1 (2007) 26 30

Ვ⩶೷ᶧṢߩᏪ㔚ߣᚻᜰᲕ⩶ߩଦㅴᄢ ᎑ ቁ ਯ㧖,1㧘ች ਅ ᓕ ๋㧖㧘ዊ ᥵ ථ ᄦ㧖㧘૒ ⮮ ᱜ ਯ㧖, ਛ ᩮ ஒ ᄦ㧖㧖㧔2006 ᐕ 9 ᦬ 15 ᣣฃઃ㧧2007 ᐕ 2 ᦬ 19 ᣣฃℂ㧕

Enhancement of Hand Cleaning by Charged Disinfectant Mist
Takayuki OHSHIMA*, Seigo MIYASHITA*, Takuo KOGURE*, Masayuki SATO*
Masayuki SATO* and Tomoo NAKANE**(Received September 15, 2006 ; Accepted February 19, 2007)

Effectiveness of liquid disinfectant mist generated by ultrasonic humidifier was studied for hand cleaning. 1 % CGH/ 61 % ethanol was most effective to achieve efficient inactivation of Escherichia coli cells on the surface of agar
medium plate. The mist of liquid disinfectant was introduced to the region of corona discharge with applying 㧙5,10, or 15 kV DC. The mist was negatively charged up electrically, and the charged mist efficiently attached to thesurface of target agar medium plate when decoloration of chloramphenicol was examined. However, excess DC
voltage, 15 kV in this study, resulted in the decrease of mist amount attached on the target than that in the case of 10kV was applied. Although the time for sterilizing bacteria on agar medium plate without DC corona was sevenminutes, it shortened to one minute with DC corona. We also examined the actual use for hand cleaning, and theenhancement of inactivating bacteria on fingers was confirmed in our hand cleaning system. After 20 seconds oftreatment, the number of bacteria cells on hand was drastically decreased with our system, and it will be applicable forhand cleaning in hospitals and fast food stores.
1.

ߪߓ߼ߦࠇࠍ㒐ᱛߔࠆߚ߼ߦᦨૐ㒢ߩ⮎೷ߢᚻᜰᲕ⩶߇น⢻ߥⵝ⟎ᚻᜰߩⴡ↢▤ℂߪක≮࡮㘩ຠ⃻႐ߦ߅޿ߡᔅ㗇ߢ޽ࠆߚ߼㧘ߩ㐿⊒߇ᦸ߹ࠇߡ޿ࠆ㧚ߘߎߢᧄ⎇ⓥߢߪᲕ⩶⮎೷ࠍࡒࠬ࠻ࡑ࠾ࡘࠕ࡞ൻߐࠇ㧘⟵ോൻߐࠇߡ޿ࠆ㧚ߎࠇࠄߩࡑ࠾ࡘࠕ࡞⁁ߦ⊒↢ߐߖ㧘ߎࠇࠄߩᶧṢࠍᏪ㔚ߐߖࠆߎߣߦࠃࠅᚻᜰ⴫

ਛߩᵞᵺ࡮㒰⩶ߩߚ߼ߩᠲ૞ߪ㕖Ᏹߦᾘℴߢ 10 ಽ⒟ᐲߩᤨ

㕙߳ߩઃ⌕ࠍଦㅴߒߡᚻᜰᲕ⩶ࠍⴕ߁ߎߣࠍ⋡⊛ߣߒߚታ㑆ࠍⷐߒ㧘ബⴕߦ࿎㔍߇઻߁1㧘2㧕㧚߹ߚ㧘ߎߩᚻᜰᲕ⩶ᠲ૞ߩ೽૞↪ߣߒߡᚻ⨹ࠇ߇⊒↢ߒ㧘ᚻ⨹ࠇㇱಽ߇㔀⩶ߩ᷷ᐥߣ
3㧕㛎⊛ᬌ⸛ࠍⴕߞߚ㧚ᧄ⎇ⓥߢߪࡒࠬ࠻ߦߒߡྃ㔵ߒߚ႐วߦㆡߒߚᲕ⩶⮎೷ߥࠆ੐଀߽ႎ๔ߐࠇߡ߅ࠅ 㧘◲ଢ߆ߟ᷷๺ߥᚻᜰᲕ⩶ᣇᴺ

ߩㆬቯࠍⴕߞߚ㧚߹ߚߎࠇࠄߩ⮎೷ᶧṢࠍ DC ࠦࡠ࠽᡼㔚႐߇ᓙᦸߐࠇߡ޿ࠆ㧚৻ᣇ㧘ක≮࡮ࡈࠔ࡯ࠬ࠻ࡈ࡯࠼ᐫߥߤߦߦ౉ࠇߚᤨߩᏪ㔚ࠍ㧘ઃ⌕ኻ⽎߳ߩઃ⌕㊂ߩᄌൻࠍ⺞ߴࠆߎ

ߪਇ․ቯᄙᢙߩੱ‫߇ޘ‬㗫❥ߦ಴౉ࠅߔࠆ㧚㒮ౝᗵᨴ߿㘩ਛᲥߣߢ⹏ଔߒߚ㧚ߐࠄߦᏪ㔚ߒߚ⮎೷ᶧṢ߇ኙᄤၭ࿾਄ߩ⩶߿

ⵍኂߩ᜛ᄢࠍ㒐ߋⷰὐ߆ࠄ㧘ߎࠇࠄߩੱ‫߽ޘ‬ᚻᜰᲕ⩶ࠍⴕߞታ㓙ߩᚻߩᲕ⩶ࠍଦㅴߔࠆ߆ߤ߁߆ታ㛎⊛ߦᬌ⸛ߒߚ㧚ߚ߶߁߇ᅢ߹ߒ޿߇㧘ਇ․ቯᄙᢙߩੱ߇ᚻシߦᚻᜰᲕ⩶ߢ߈ࠆⵝ⟎࡮⸳஻ߪ⊝ήߦ╬ߒ޿㧚ߎߩࠃ߁ߥ⢛᥊߆ࠄ⍴ᤨ㑆ߦᚻᜰᲕ⩶߇น⢻ߢ㧘߆ߟᚻ⨹ࠠ࡯ࡢ࡯࠼㧦ᚻᜰ‫ޔ‬Ვ⩶‫࠽ࡠࠦޔ‬᡼㔚‫ޔ‬ᶧṢ‫⿥ޔ‬㖸ᵄᝄേሶ* ⟲㚍ᄢቇᎿቇㇱ↢‛ൻቇᎿቇ⑼㧔376-8515 ⟲㚍⋵᩿↢Ꮢᄤ␹↸ 1㧙5㧙1㧕
Department of Biological and Chemical Engineering, GunmaUniversity, 1-5-1 Tenjin-cho, Kiryu 376-8515, Japan** ᣣᧄᄢቇ↢↥Ꮏቇㇱ㔚᳇㔚ሶᎿቇ⑼㧔275-8575 ජ⪲⋵⠌ᔒ
㊁Ꮢᴰ↸ 1-2-1㧕
Department of Erectrical and Electronic Engineering, College ofIndustrial Technology, Nihon University, 1-2-1 Izumi-cho,Narashino, Chiba 275-8575, Japan1
tohshima@bce.gunma-u.ac.jp2.

ታ㛎ᣇᴺ࡮ᚻ㗅2.1ታ㛎ⵝ⟎

࿑ 1 ߦᧄ⎇ⓥߢ↪޿ߚታ㛎ⵝ⟎ߩ᭎⇛࿑ࠍ␜ߔ㧚ᚻᜰᲕ⩶↪ߩᶧ೷ࠍ⿥㖸ᵄᝄേሶ㧔⿥㖸ᵄടḨེ USH-400ECO㧘⑺᦬
㔚ሶ㧕ࠍᐩߦ㈩⟎ߒߚ౞╴ߦቯᏱㆇォ᳓૏߹ߢᲕ⩶⮎೷߿ઁߩ᳓ṁᶧࠍ౉ࠇߚ㧚⿥㖸ᵄᝄേሶߦߪ AC45 V ࠍ౉ജߒ㧘400mL/mim ߢ㔵ൻࡏ࠶ࠢࠬ㧔ࠕࠢ࡝࡞⵾㧘70100100mm㧕ౝߦ㔵ൻߐߖߚ㧚㔵ൻࡏ࠶ࠢࠬߦߪ DC ࡈࠔࡦ㧔12 V㧕߇ขࠅઃߌߡ޽ࠅ㧘㔵ൻߒߚ⮎೷ᶧṢࠍ᡼㔚౞╴ߦㅍࠅㄟࠎߢ޿ࠆ㧚᡼㔚౞╴ߪࠕࠢ࡝࡞౞╴㧔45I65 mm㧕ߩ஥㕙ߦ㊎㔚ᭂࠍᝌ

27౉ߒ㧘౞╴෻ኻ஥ߦ㌃ࠪ࡯࡞ࠍ⾍ࠅઃߌߡࠕ࡯ࠬ㔚ᭂߣߒߚ㧚

2.4

ශട㔚࿶ߪ DC ࡑࠗ࠽ࠬ 5㧘10㧘߅ࠃ߮ 15 kV ߢ޽ߞߚ㧚᡼

Ვ⩶ኻ⽎ߣߒߡߪᄢ⣺⩶㧔Escherichia coli K-12㧕㧘߅ࠃ߮࠼㔚౞╴ࠍㅢㆊߒߚ⮎೷ᶧṢߪಣℂᮏࡏ࠶ࠢࠬߦㅍࠄࠇ㧘ࡏ࠶࡜ࠗࠗ࡯ࠬ࠻㧔ਃ౒ࠗ࡯ࠬ࠻㧕ࠍ૶↪ߒߚ㧚ᄢ⣺⩶ߪኙᄤၭ

ࠢࠬ஥㕙ߦขࠅઃߌߚ㌃᧼㧔ࠕ࡯ࠬ㔚ᭂ㧕
㧘90mmIࠪࡖ࡯࡟㧘

࿾਄ߩᲕ⩶⹏ଔߦ૶↪ߒߚ㧚߹ߚࠗ࡯ࠬ࠻⩶ߪታ㓙ߩᚻᜰᲕ߅ࠃ߮ᚻߦኻߔࠆઃ⌕᜼േࠍⷰኤߒߚ㧚⩶ߩ⹏ଔߦ૶↪ߒߚ㧚

2.2

ᚻᜰᲕ⩶↪ᶧ೷

Ვ⩶⹏ଔᄢ⣺⩶ߪ੍߼ LB ᶧ૕ၭ࿾㧔BACTO yeast extract 10 g/L,ᚻᜰᲕ⩶↪ߩᶧ೷ߣߒߡߪ 0.5 㧑 CGH㧔chlorhexidine

BACTO peptone 10 g/L, NaCl 5 g/L pH 7.2㧕ߢ 37͠㧘12 ᤨ㑆ᝄgluconate, ๺శ⚐⮎㧕᳓ṁᶧ㧘1 % NaClO㧔ᰴ੝Ⴎ⚛㉄࠽࠻࡝ߣ߁ၭ㙃ߒߚၭ㙃ᶧࠍ↪ᗧߒ㧘104 CUF/mL ⒟ᐲߦߥࠆࠃ߁࠙ࡓ㧘๺శ⚐⮎㧕᳓ṁᶧ㧘70 % ethanol ᳓ṁᶧ㧘߅ࠃ߮ 1 % CGHߦᏗ㉼ߒߚ⩶ Ộᶧࠍ⺞ᢛߒߚ㧚ߎߩ⩶ Ộᶧ 0.1 mL ࠍࠪࡖ/ 61 % ethanol ᳓ṁᶧࠍታ㛎⋥೨ߦ⺞ᢛߒߡ↪޿ߚ㧚࡯࡟ߦ⺞ᢛߒߚ LB ኙᄤၭ࿾㧔BACTO yeast extract 10 g/L,2.3

ઃ⌕ߩⷰኤᣇᴺ࡮᷹ቯᣇᴺ

ࠦࡠ࠽᡼㔚႐ࠍㅢㆊߒߚᶧ೷ᶧṢߩઃ⌕ଦㅴലᨐࠍⷰኤ

BACTO peptone 10 g/L, NaCl 5 g/L, agar 15 g/L pH 7.2㧕ߦႣᏓߒߡࠨࡦࡊ࡞ߣߒߚ㧚ߔࠆߚ߼ߦ㧘ኙᄤߣࠠࡓࡢࠗࡊࠍ↪޿ߚᣇᴺࠍⴕߞߚ㧚ኙᄤ߹ߚ࠼࡜ࠗࠗ࡯ࠬ࠻ߩ႐ว㧘1.5 g ࠍṌ⩶᳓ߦ Ộߒ㧘Ꮧ㉼ࠍ↪޿ߚᣇᴺߪ 0.01 M NaOH㧘ࡈࠚࡁ࡯࡞ࡈ࠲࡟ࠗࡦᜰ␜⮎ࠍⴕߞߡ⚂ 103 CFU/mL ߣߥࠆࠃ߁ߦᏗ㉼ߒߚ⩶ Ộᶧࠍ⺞ᢙṢࠍ฽߻ 1 %ኙᄤࡊ࡟࡯࠻ࠍ 90 mm Iࠪࡖ࡯࡟ߦ⺞ᢛߒ㧘ᢛߒߚ㧚߹ߚታ㓙ߩⵍ㛎⠪ߩᚻࠍࠢ࡝࡯ࡦࡌࡦ࠴ౝߢ 70 %ᶧ೷ߣߒߡ 0.1 M ㈶㉄ࠍ㔵ൻߒߡ૶↪ߒߚ㧔࿑ 1㧕
㧚㈶㉄ṁᶧethanol ṁᶧߢឪߺᵞ޿ߒߡᚻ⴫㕙ࠍṌ⩶ߒߚ㧚Ṍ⩶ߒߚᚻߩߩઃ⌕ߦ઻߁ࡈࠚࡁ࡯࡞ࡈ࠲࡟ࠗࡦ⦡ߩ⣕⦡ᄌൻࠍⷰኤߒੱᏅߒᜰ╙৻㑐▵߹ߢࠍ⺞ᢛߒߚࠗ࡯ࠬ࠻ Ộᶧߦᶐߒ㧘ࠗߚ㧚

࡯ࠬ࠻⩶ࠍᜰߦઃ⌕ߐߖߚ㧚චಽߦᶐߒߚᓟ㧘࠼࡜ࠗࡗ࡯ߢ߹ߚࠪࡖ࡯࡟ਛߦࠕ࡯ࠬߦធ⛯ߒߚࠠࡓࡢࠗࡊ 㧔506020 ⑽㑆ੇ῎ߐߖߚ㧚ࠗ࡯ࠬ࠻ࠍઃ⌕ߐߖߚᚻࠍ࿑ 1 ߩⵝ⟎ߦmm㧕ࠍ߅߈㧘0.5 㧑 CGH ᳓ṁᶧࠍ㔵ൻߒߡ૶↪ߒߚ㧚ࠠࡓ࿑ߩࠃ߁ߦ 20 ⑽㑆ᝌ౉ߒߚ㧚ಣℂᓟߩᚻߪṌ⩶ߒߚ㤈⧘ኙࡢࠗࡊ ߪታ㛎ಣℂߦ઻޿ḨẢߒߡߊࠆߚ߼ࠕ࡯ࠬߦߥࠆ

ᄤၭ࿾㧔ᣣ᳓㧕ࠍ 40͠߹ߢ಄ළߒߚṁᶧౝߢࠃߊᵞ޿㧘㤈⧘ߣ⠨߃ࠄࠇࠆ㧚৻ቯᤨ㑆ᓟߩࠠࡓࡢࠗࡊ ߩ㊀㊂ᄌൻࠍⷰኤ

ኙᄤၭ࿾ࠍߘߩ߹߹಄ළ࿕ൻߒߚᓟ㧘ࠗࡦࠠࡘࡌ࡯࠲࡯ߦ⒖ߒߚ㧚ታ㛎ߪ㧟࿁ⴕ޿㧘ᐔဋ୯ࠍ᳞߼ߡ⹏ଔߒߚ㧚
ಣℂᓟߩࠨࡦࡊ࡞ߪߘߩ߹߹ 37͠ߩࠗࡦࠠࡘࡌ࡯࠲࡯ߦ
߅޿ߡ 12 ᤨ㑆ၭ㙃ߒ㧘ࠦࡠ࠾࡯ᒻᚑᢙ
㧔Colony Formation Unit;
CFU㧕ࠍࠞ࠙ࡦ࠻ߒߡ⹏ଔߒߚ㧚3.

ታ㛎⚿ᨐ߅ࠃ߮⠨ኤ

3.1

Ვ⩶ᶧ೷ߩᬌ⸛

߹ߕ࿑ 1 ߩታ㛎ⵝ⟎ߢ᡼㔚౞╴ߦ㜞㔚࿶ࠍශടߒߥ޿᧦ઙߢ㧘ኙᄤၭ࿾਄ߩᄢ⣺⩶߇ߤߩ⒟ᐲᱫṌߐߖࠄࠇࠆ߆ࠍ⺞ߴߚ㧚↪޿ߚᶧ೷ߪ 0.5 㧑 CGH㧘1 % NaClO ᳓ṁᶧ㧘70 %ethanol ᳓ṁᶧ㧘߅ࠃ߮ 1 % CGH / 61 % ethanol ᳓ṁᶧߩ 4 ⒳㘃ߢ޽ࠆ㧚࿑ 2 ߦߎߩ⚿ᨐࠍ␜ߔ㧚ೋᦼᄢ⣺⩶ᢙߪ߅ࠃߘ
3,000 CFU/plate ೨ᓟߢ޽ࠅ㧘޿ߕࠇߩ⮎೷ߢ߽ᤨ㑆ߣ౒ߦ↢⩶ᢙ߇ૐਅߒߡ޿ߚ㧚ᦨ߽Ვ⩶ലᨐ߇ዊߐ߆ߞߚߩߪ∛㒮ౝߩṌ⩶ᵞᵺ೷ߣߒߡ↪޿ࠄࠇߡ޿ࠆCGH ߩ0.5 %ṁᶧߢ޽ࠅ㧘
15 ಽᓟߦߪ↢⩶ᢙߪ 1/3 એਅߦߥߞߡ޿ߚ㧚ߘߩઁߩᶧ೷ߢ
ߪߘࠇߙࠇቢోߦᱫṌߐߖࠆߎߣ߇ߢ߈㧘70 % ethanol ᳓ṁ࿑ 1 ታ㛎ⵝ⟎᭎⇛࿑ᶧߢߪ 12 ಽᓟ㧘
1 % NaClO ᳓ṁᶧߢߪ 9 ಽᓟ㧘
1 % CGH / 61 %Fig.1 Experimental set up.

ethanol ߢߪ 7 ಽᓟߦߪ↢⩶߇⹺߼ࠄࠇߥߊߥߞߡ޿ߚ㧚Groveߒ㧘㊀㊂Ⴧࠍᶧ೷ઃ⌕㊂ߣߒߡ⹏ଔߒߚ㧚ࠄ㧘Mulberrry ࠄߪਥߦ∛㒮ౝߩᲕ⩶⮎೷ߣߒߡ 1 % CGH /61 % ethanol ߇ᦨ߽ലᨐ⊛ߢ޽ࠆߣㅀߴߡ޿ࠆ 4㧘5㧕㧚ߎࠇࠄߩ

283112007

࿑ 3 ࠠࡓࡢࠗࡊ ߦઃ⌕ߒߚ 0.5 㧑 CGH ߩ㊀㊂
࿑ 2 ฦ⒳⮎೷ྃ㔵ߦࠃࠆᄢ⣺⩶⩶ᢙߩᷫዋ

ᄌൻ

Fig.2 Inactivation of E. coli by various disinfectant mist.

Fig.3 Mass of 0.5 㧑 CGH attached mist on sheets ofKimwipe.

ታ㛎ߪᶧ೷ߩਛߢᚻࠍឪߺᵞ޿ߔࠆߎߣࠍ೨ឭߣߒߚታ㛎ߢ޽ߞߚ߇㧘ᧄ⎇ⓥߢⴕߞߡ޿ࠆ㔵ൻᣇᑼߢ߽ 1 % CGH /ⷞߢ᷹ⷰߒߚߣߎࠈ㧘15 kV ࠍශടߒߚ႐วߦߪㅢㆊߔࠆᶧ

61 % ethanol ߇ᦨ߽ലᨐ⊛ߢ޽ࠆߎߣ߇⏕⹺ߢ߈ߚ㧚ߒ߆ߒ㧘Ṣ߇᣿ࠄ߆ߦᷫዋߒߡ޿ࠆߎߣࠍ⏕⹺ߒߚ㧚ߎࠇߪࠦࡠ࠽᡼㔵ൻᣇᑼߢኙᄤၭ࿾਄ߩᄢ⣺⩶ࠍቢోߦᱫṌߐߖࠆߚ߼ߦ

㔚ߦ઻߁ EHD ᵹࠇߦࠃࠅᶧṢ߇ᢔㅺߒߡߒ߹߁ߎߣߦട߃㧘ߪ 7 ಽ㑆ᔅⷐߢ޽ࠆߎߣ߇ࠊ߆ߞߚ㧚ᧄ⎇ⓥߩ⋡⊛ߪ૶↪ߔᏪ㔚ߒߚᶧṢ߇ࠕ࡯ࠬߦᒁ߈ነߖࠄࠇߚߚ߼㧘ࠦࡠ࠽᡼㔚႐ࠆ⮎೷㊂ߩૐᷫߣಣℂᤨ㑆ߩ⍴❗ߢ޽ࠆ㧚ᶧ೷ࠍࡒࠬ࠻⁁ߦࠍㅢㆊߢ߈ࠆᶧㆡ㊂߇ᷫዋߒߚߚ߼ߣ⠨߃ࠄࠇࠆ㧚ߘߎߢ੹⭯ߊᢔᏓߔࠆߎߣߢ૶↪㊂ߪᦨૐ㒢ߦߢ߈ࠆߣ⠨߃ࠄࠇࠆ㧚ᓟߩታ㛎ߪශട㔚࿶ 10 kV એਅߢⴕ߁ߎߣߣߒߚ㧚

ߒ߆ߒਇ․ቯᄙᢙߩੱ߇᳇シߦⴕ߁ߚ߼ߦߪ߽ߞߣ⍴ߊߔ

࿑ 4 ߪ 0.01 M NaOH㧘ࡈࠚࡁ࡯࡞ࡈ࠲࡟ࠗࡦᜰ␜⮎ᢙṢࠆᔅⷐ߇޽ࠆߣ⠨߃ࠄࠇࠆ㧚ߘߎߢಣℂᤨ㑆 1 ಽએౝࠍ⋡ᮡࠍ฽߻1 %ኙᄤࡊ࡟࡯࠻ߦ0.1 M ㈶㉄ṁᶧࠍ5 ಽ㑆ྃ㔵ߒߚߦታ㛎ࠍⴕ߁ߎߣߣߒߚ㧚႐วߩ⣕⦡ߩ⚻ᤨᄌൻࠍ␜ߒߡ޿ࠆ㧚ήශടߩ႐วߦߪ 3 ಽ3.2ᓟߦ⿒⦡߇⭯ߊߥࠅᆎ߼㧘એᓟᓢ‫ోߦޘ‬૕⊛ߦ⭯ߊߥߞߡ޿ᶧ೷ᶧṢߩᏪ㔚ߦࠃࠆઃ⌕ଦㅴ⿥㖸ᵄᝄേሶߦࠃࠆᶧ೷ߩ㔵ൻߦࠃߞߡ߽ኙᄤၭ࿾਄ߩߊ௑ะߢ޽ߞߚ㧚৻ᣇ㧘㔚࿶ࠍ 5㧘10 kV ශടߒ㧘ኙᄤࡊ࡟ᄢ⣺⩶ࠍᱫṌߐߖࠆߎߣ߇⏕⹺ߐࠇߚ߇㧘ࠃࠅ⍴ᤨ㑆ߢಣℂ࡯࠻ࠍࠕ࡯ࠬߒߚ႐วߦߪ㧔౮⌀਄ᣇߩ྾ⷺ޿㌃ ߇ࠕ࡯ࠍߔࠆߎߣࠍ⠨߃㧘⮎೷ᶧṢࠍᏪ㔚ߐߖߡઃ⌕ࠍଦㅴߔࠆߎࠬ㧕᣿ࠄ߆ߦ⣕⦡ߩଦㅴ߇ⷰኤߐࠇߚ㧚5 kV ߩᤨߦߪ 1 ಽߣࠍ⠨߃ߚ㧚߹ߕ 0.5 㧑 CGH ᳓ṁᶧࠍ⮎೷ߣߒߚ႐วߩࠠᓟߦߪࠕ࡯ࠬㄭறߩ⣕⦡߇⹺߼ࠄࠇᆎ߼㧘3 ಽᓟߦߪቢోߦ

ࡓࡢࠗࡊ਄߳ߩઃ⌕㧔㊀㊂Ⴧ㧕ࠍⷰኤߒߚ㧚࿑㧟ߦߪශട㔚⣕⦡ߒߚ㗔ၞ߇⃻ࠇߡ޿ߚ㧚5 ಽᓟߦߪ๟ㄝㇱࠍ㒰޿ߡ߶߷࿶ࠍ 0,5,10,15 kV ශടߒߡࠦࡠ࠽᡼㔚ࠍㅢㆊߒߚ 0.5 㧑 CGቢోߦ⣕⦡ߐࠇߡ޿ߚߩߦട߃㧘㈶㉄ṁᶧ߇ኙᄤࡊ࡟࡯࠻਄H ᳓ṁᶧᶧṢߩ㊀㊂Ⴧടߩ⚻ᤨᄌൻࠍ␜ߒߡ޿ࠆ㧚0㨪10 kߦઃ⌕ߒߡ޿ࠆ᭽ሶ߇⏕⹺ߢ߈ߚ㧚 10 kV ශടߒߚ႐วߦV ߹ߢߪශട㔚࿶ߦ઻޿㊀㊂Ⴧട߇ᄢ߈ߊߥߞߡ޿ߚ㧚ήශ

߽ 5 kV ߩ႐วߣห᭽ߩ௑ะߢ޽ߞߚ߇㧘ࠃࠅᣧߊ⣕⦡ߒߡട㧔0 kV㧕ߩߣ߈ߦߪ㧝ಽᓟ߹ߢߪ᦭ᗧߥઃ⌕ߪ⹺߼ࠄࠇߕ㧘޿ࠆߎߣ߇⏕⹺ߐࠇߚ㧚߹ߚ 10 kV ߩ႐วߦߪ 3 ಽᓟߦߪ㈶3 ಽᓟߦߪ 0.05 g ߩᶧ೷߇ઃ⌕ߒߡ޿ߚ㧚ߎࠇߦኻߒ㔚࿶ࠍ㉄ṁᶧߩઃ⌕߇⋡ⷞߢ߈ߚ㧚ߐࠄߦ 5 ಽᓟߩ๟ㄝㇱߩᧂ⣕⦡ශടߒߚ႐วߦߪ᣿ࠄ߆ߦઃ⌕ߩଦㅴ߇⏕⹺ߐࠇ㧘10 kV ශ㗔ၞ߽ 5 kV ߩ႐วࠃࠅ߽ዊߐ߆ߞߚ㧚߹ߚࠕ࡯ࠬߒߚ႐ว

ട 1 ಽᓟߦ 0.14 g㧘
3 ಽᓟߦߪ 0.27 g ߩᶧṢ߇ઃ⌕ߒߡ޿ߚ㧚ߣߒߥ߆ߞߚ႐วߢᲧセࠍⴕߞߚ߇㧘ࠕ࡯ࠬߒߥ߆ߞߚ႐วࠦࡠ࠽᡼㔚ߦࠃࠅᶧṢ߇Ꮺ㔚ߔࠆߎߣߦࠃࠅ㧘ઃ⌕߇ଦㅴߐߢ߽⣕⦡ߩଦㅴ߇⷗ࠄࠇߚߚ߼㧘੹ᓟߩታ㛎ߢߪࠕ࡯ࠬࠍⴕࠇߚߣ⠨߃ࠄࠇࠆ㧚ߒ߆ߒ 15 kV ࠍශടߒߚ႐วߦߪ 10 kVࠊߥ޿ߎߣߣߒߚ㧚ࠍශടߒߚ႐วࠃࠅઃ⌕㊂߇ᷫዋߒߡ޿ߚ㧚᡼㔚౞╴ౝࠍ⋡- 3 -࡜ࡦ࠾ࡦࠣ࠲ࠗ࠻࡞㧔⪺⠪ฬ㧘ߚߛߒⶄᢙߩ႐วవ㗡⪺⠪㧗ࠄ㧕

29

࿑ 4 0.1M ㈶㉄ࡒࠬ࠻ߦࠃࠆࠢࡠ࡜ࡓࡈࠚ࠾ࠦ࡯࡞ߩ⣕⦡(A)ήශട㧘(B)5 kV㧘(C)10 kV
Fig.4 Decoloration of chloramphenicol due to 0.1 M acetic acid mist, (A)0 kV㧘(B)5 kV㧘(C)10 kV.
3.3

Ꮺ㔚ߒߚ 1 % CGH / 61 % ethanol ᶧṢߦࠃࠆኙᄤ

ߒ㧘ኙᄤၭ࿾ࡊ࡟࡯࠻߳ߩઃ⌕߇ଦㅴߐࠇ㧘⴫㕙ߩᄢ⣺⩶߇ࡊ࡟࡯࠻߅ࠃ߮ᚻᜰ⴫㕙ߩᲕ⩶ᱫṌߒߚߣ⠨߃ࠄࠇࠆ㧚

LB ኙᄤၭ࿾ࡊ࡟࡯࠻ߦ 103㨪104 CFU ߩᄢ⣺⩶ࠍႣᏓߒߚᰴߦߎࠇ߹ߢߩታ㛎⚿ᨐࠍታ⸽ߔࠆߚ߼ߦ㧘ታ㓙ߩᚻᜰᲕࠨࡦࡊ࡞ߦኻߒ㧘1 % CGH / 61 % ethanol ᶧṢࠍྃ㔵ߒߚᤨߩ⩶߳ߩㆡ↪ࠍ⠨߃ߚ㧚ታ㛎ᣇᴺߢㅀߴߚࠃ߁ߦታ㓙ߩⵍ㛎⠪⚻ᤨᄌൻࠍ࿑ 5 ߦ␜ߔ㧚㔚࿶ήශടߩ႐วߪᤨ㑆ߣߣ߽ߦߩᜰߦࠗ࡯ࠬ࠻⩶ࠍઃ⌕ߐߖ㧘10 kV 㔚࿶ශടࠍⴕߞߚ 1 %2ᓢ‫ߦޘ‬ᷫዋߒ㧘60 ⑽ᓟߦߪ 10 CFU ⒟ᐲ߹ߢᷫዋߒߡ޿ߚ㧚

CGH / 61 % ethanol ᶧṢࠍ 20 ⑽㑆ྃ㔵ߐߖߚ㧚ߎߩ⚿ᨐࠍ࿑ߎࠇߦኻߒ㧘10 kV ශടߒߚ႐วߦߪᄢ⣺⩶ߩᷫዋ߇㗼⪺ߢ㧘

6 ߦ␜ߔ㧚ᚻᜰߦᱷሽߔࠆࠗ࡯ࠬ࠻⩶ᢙࠍࠞ࠙ࡦ࠻ߒߚߣߎ

60 ⑽ᓟߦߪᬌ಴ߐࠇߥߊߥߞߡ޿ߚ㧚ߎߩታ㛎ࠃࠅ 1 % CGH

ࠈ㧘ήಣℂߩ႐ว߇߅ࠃߘ 3,800 CFU ߢ޽ߞߚߩߦኻߒ㧘ή/ 61 % ethanol ᶧṢ߇ࠦࡠ࠽᡼㔚႐ㄭறࠍㅢㆊߔࠆߎߣߢᏪ㔚ශടߩ႐ว߇߅ࠃߘඨಽߩ 1950 CFU ߢ޽ߞߚ㧚
10 kV ශടߒ

࿑ 5 ኙᄤၭ࿾਄ߩᄢ⣺⩶ߩᲕ⩶㧔A㧕ήශട㧘㧔B㧕10 kV ශട
Fig.5 Inactivation of E. coli on the surface of agar medium, (A) 0 kV, (B) 10 kV.
- 4 -3031120072㧕
㧚ߎࠇߦኻߒᏪ㔚ߐߖߚ႐วߦߪ⚂ 1 ಽ(࿑ 5)ߣ㧝㧛㧣ߦ⍴❗ߢ߈ߚ㧚ઃ⌕㊂ࠍ᷹ቯߒߚ࿑㧟ߪࠠࡓࡢࠗࡊ 㧔5060mm㧕ߢ޽ߞߚ߇㧘1 ಽᓟߩઃ⌕㊂ߪ 0.14 g ߢ޽ߞߚ㧚90mmIࠪࡖ࡯࡟ߦ឵▚ߔࠆߣ⚂ 0.3 g ߢ޽ࠅ㧘㕖Ᏹߦዋ㊂ߩ⮎೷߇ή㚝ߥߊ᦭ലߦᲕ⩶ߦነਈߒߡ޿ࠆߎߣ߇ផቯߐࠇࠆ㧚߹ߚታ㓙ߩᚻࠍ↪޿ߚታ㛎ߢ߽ 20 ⑽㑆ߢࠗ࡯ࠬ࠻⩶ᢙࠍ㧝㧛㧝㧜ߦᷫዋߐߖࠆߎߣ߇น⢻ߢ޽ࠆߎߣ߽ࠊ߆ߞߚ㧔࿑ 6㧕㧚ੱߩᚻߩ⴫㕙ߪኙᄤၭ࿾ࡊ࡟࡯࠻ߩࠃ߁ߥṖࠄ߆ߥ⴫㕙ߢߪߥߊ㧘Ḵߩㇱಽߦ㔀⩶߇ᱷሽߔࠆߣ⠨߃ࠄࠇࠆ߇㧘ࠊߕ߆ 20⑽㑆ߢ㧘߆ߟዋߥ޿⮎೷ߢ഍⊛ߦ㔀⩶ᢙࠍᷫዋߐߖࠄࠇࠆߎ
ߣߪታ↪਄ᭂ߼ߡଔ୯߇㜞޿ߣ⠨߃ࠄࠇࠆ㧚∛㒮㧘ࡈࠔ࡯ࠬ࠻ࡈ࡯࠼ᐫߥߤߦ಴౉ࠅߔࠆਇ․ቯᄙᢙߩੱ߇㧘ᚻ⨹ࠇࠍ᳇ߦߔࠆߎߣߥߊ◲නߥᣇᴺߢᚻᜰᲕ⩶ߢ߈ࠆⵝ⟎㐿⊒߇น⢻ߦߥࠆߣ⠨߃ࠄࠇࠆ㧚࿑ 6 ᚻᜰߦઃ⌕ߐߖߚࠗ࡯ࠬ࠻⩶ߩᲕ⩶4.

Fig.6 Inactivation of yeast cells on hand.

߹ߣ߼ᧄ⎇ⓥߢߪ⿥㖸ᵄᝄേሶߦࠃࠆ⮎೷ߩᶧṢྃ㔵ᣇᴺࠍᬌߚ႐วߪ 400 CFU એਅߢ㧘ⵍ㛎⠪ࠍࠕ࡯ࠬߒߚ႐วߣߒߥ޿⸛ߒߚ㧚⮎೷ߩ⒳㘃ߣߒߡߪ 1 % CGH / 61 % ethanol ᶧṢ߇႐วߢ㆑޿ߪ⹺߼ࠄࠇߥ߆ߞߚ㧚ߎߩ⚿ᨐࠃࠅᏪ㔚ߒߚ 1 %㜞޿Ვ⩶⢻ജࠍ␜ߒߚ㧚ߎߩ⮎೷ᶧṢࠍ㧘ࠦࡠ࠽᡼㔚႐ࠍㅢ

CGH / 61 % ethanol ᶧṢߪࠕ࡯ࠬߩ᦭ήߦ߆߆ࠊࠄߕᚻᜰߦㆊߐߖࠆߎߣߢᏪ㔚ߒ㧘ࠠࡓࡢࠗࡊ 㧘ኙᄤࡊ࡟࡯࠻߳ߩઃኻߒߡ߽ࠃߊઃ⌕ߒ㧘⴫㕙ߩࠗ࡯ࠬ࠻⩶ࠍߔ߫߿ߊᲕ⩶ߢ߈

⌕ଦㅴ߇⏕⹺ߢ߈ߚ㧚ߎߩᣇᑼߦࠃࠅኙᄤၭ࿾ࡊ࡟࡯࠻਄ߩࠆߎߣ߇ታ⸽ߢ߈ߚ㧚∛㒮߿㘩ຠᬺ⇇ߢ໧㗴ߣߥࠆ∛ේ⩶ߪᄢ⣺⩶ߩᲕ⩶ࠍଦㅴߔࠆലᨐ߇⏕⹺ߢ߈㧘Ṍ⩶ߔࠆߩߦᔅⷐ

ήᢙߦ޽ࠆ㧚ߎࠇࠄߩ∛ේ⩶વ᠞ߩේ࿃ߣߒߡᚻߩ੺࿷ߪᄢ

ߥᤨ㑆ࠍ㧝㧛㧣ߦ⍴❗ߢ߈ߚ㧚߹ߚታ㓙ߩੱߩᚻᜰᲕ⩶ߦ߅

߈޿㧢㧕㧚ߎࠇࠍ㒐ᱛߔࠆߚ߼ߦߪᚻࠍή⩶ൻߔࠆߎߣ߇ℂᗐ޿ߡ߽ଦㅴ߇⏕⹺ߢ߈ߚ㧚ᧄᣇᑼߩᚻᜰᲕ⩶ߪ૶↪ߔࠆ⮎೷⊛ߢ޽ࠆ߇㧘⃻ታ⊛ߢߪߥ޿㧚ᒝജߥᲕ⩶೷ࠍ↪޿ߡ㧘චಽ

ߩዋ㊂ൻ߅ࠃ߮ᤨ㑆ߩ⍴❗ലᨐ߇ᦼᓙߢ߈ࠆ㧚

ߦಣℂߒߚߣߒߡ߽ᚻߩḴᷓߊߦሽ࿷ߒߡ޿ࠆ⩶߹ߢᲕ⩶ߔࠆߩߪኈᤃߢߪߥ޿㧚⃻ታ⊛ߥ㒐ᱛᣇᴺߣߒߡߪᧄ⎇ⓥߢ

ෳ⠨ᢥ₂
G. Kampf and C. Ostermeyer: J. Hospital Infect. 52 (2002) 2192) R. Montville, Y. Chen, and D. W. Schaffner: Int. J. Food
Microbiol. 73 (2002) 305
3) C. Marena, L. Lodola, M. Zecca, A. Bulgheroni, E. Carretto, R. Maserati and L. Zambianchi: American J. Infect.
Control, 30 (2002) 334
4) G.L. Grove, C.R. Zerweck, J.M. Heilman, and J.D. Pyrek:
American J. Infect. Control, 29 ( 2001) 361
5) G. Mulberrry, A. T. Snyder, J. Heilman, J. Pyrek, and J.Stahl: American J. Infect. Control, 29 (2001) 377
6) ᧻ᧄ ᘮ⬿ ✬‫∛ޟ‬ේ⩶ߩ੹ᣣ⊛ᗧ๧‫ޠ‬ᡷ⸓㧟  ක⮎ࠫࡖ࡯࠽࡞␠ (2003)1)ឭ᩺ߒߡ޿ࠆࠃ߁ߥ◲නߥⵝ⟎࡮ᚻ㗅ߢ⍴ᤨ㑆ߩಣℂࠍ➅ࠅ㄰ߒⴕߞߡᚻߩ⩶ᢙࠍᷫዋߒߟߠߌࠆ㧘޽ࠆ޿ߪ⩶ᢙ߇ૐ޿⁁ᘒࠍ⛽ᜬߔࠆߎߣ߇∛ේ⩶ߦࠃࠆⵍኂࠍૐᷫߐߖࠆߚ߼
ߩ᦭ലߥᣇᴺߢߪߥ޿߆ߣ⠨߃ߡ޿ࠆ㧚ᧄ⎇ⓥߢߪㄦㅦߥᚻᜰᲕ⩶ࠍ⋡⊛ߦ㧘⿥㖸ᵄᝄേሶߦࠃࠆ⮎೷ߩᶧṢྃ㔵ᣇᴺࠍᬌ⸛ߒߚ㧚⮎೷ᶧṢྃ㔵ᣇᑼߢ߽ 1 %CGH / 61 % ethanol ᶧṢ߇㜞޿Ვ⩶⢻ജࠍ␜ߒߚ㧚ߒ߆ߒࠦࡠ࠽᡼㔚ࠍⴕߞߡᶧṢߩᏪ㔚ߐߖߡ޿ߥ޿႐วߦߪኙᄤၭ࿾ࡊ࡟࡯࠻਄ߩᄢ⣺⩶ࠍṌ⩶ߔࠆߩߦ⚂ 7 ಽᔅⷐߢ޽ߞߚ㧔࿑- 5 -