Peptide Antisera and Monoclonal Antibodies (1)– 259 –

Peptide Antisera andPeptide Antisera of Peptide Institute, Inc. ………… 260

Monoclonal Antibodies

Peptide Antisera ofPeptide Institute, Inc.

Peptide Antisera of Peptide Institute, Inc.
Code

Product

Host (Animal)Page

14278-v

Adrenomedullin (Human) Antiserum

Rabbit261

14438-v

Adrenomedullin 2 (Human, 1-7) Antiserum

Rabbit261

14220-v

Amylin (Rat) Antiserum

Rabbit262

14307-v

Amyloid β-Protein (Human, 1-40) Antiserum

Rabbit262

14359-v

Amyloid β-Protein (Human, 1-16) Antiserum

Rabbit262

14356-v

Amyloid β-Protein (Human, 34-40) Antiserum

Rabbit262

14357-v

Amyloid β-Protein (Human, 37-42) Antiserum

Rabbit263

14414-v

Amyloid β-Protein (Human, 37-43) Antiserum

Rabbit263

14135-v

ANP (Human, 1-28) Antiserum

Rabbit263

14224-v

Big Endothelin-1 (Human, 22-38) Antiserum

Rabbit267

14210-v

Big Endothelin-1 (Porcine, 22-39) Antiserum

Rabbit267

14238-v

Big Endothelin-2 (Human, 22-37) Antiserum

Rabbit268

14225-v

Big Endothelin-2 (Human, 22-38) Antiserum

Rabbit268

14226-v

Big Endothelin-3 (Human, 22-41 Amide) Antiserum

Rabbit268

14200-v

BNP-26 (Porcine) Antiserum

Rabbit264

14051-v

Calcitonin (Human) Antiserum

Rabbit264

14176-v

CCK-33 (Porcine) Antiserum

Rabbit26414160-v

CGRP (Human) Antiserum

Rabbit264

14233-v

CINC-1/gro (Rat) Antiserum

Rabbit265

14250-v

CNP-22 (Human) Antiserum

Rabbit265

14136-v

CRF (Human) Antiserum

Rabbit265

14338-v

β-Defensin-2 (Human) Antiserum

Rabbit266

14243-v

Elafin (Human) Antiserum

Rabbit266

14198-v

Endothelin-1 (Human) Antiserum

Rabbit267

14244-v

Galanin (Rat) Antiserum

Rabbit269

14127-v

GRF (Human) Antiserum

Rabbit269

14164-v

GRP (Human) Antiserum

Rabbit269

14802-v

Lysenin Antiserum

Rabbit270

14319-v

Midkine (Human, 1-59) Antiserum

Rabbit270

14158-v

NPY (Human) Antiserum

Rabbit270

14346-v

Orexin-A (Human) Antiserum

Rabbit270

14276-v

Osteocalcin (Human, 38-49) Antiserum

Rabbit271

3314186-v[Pyr ]-Pancreastatin (Porcine, 33-49) Antiserum

Rabbit271

14439-v

Proangiotensin-12 (Rat) Antibody

Rabbit271

14418-v

Salusin-β (Human) Antiserum

Rabbit271

14112-v

Secretin (Porcine) Antiserum

Rabbit272

14216-v

SPAI-1 (Porcine) Antiserum

Rabbit272

14365-v

Urotensin II (Human) Antiserum

Rabbit272

14110-v

VIP Antiserum

Rabbit272– 260 –

Peptide Antisera of Peptide Institute, Inc.
Our undiluted antisera are suitable for immunohistochemical use, for application to RIA or other non-isotopic
immunoassay purposes. These antisera are partially characterized by either of the following two methods:
1) EIA (see the instruction model 1 on page XI (XXIII)) or 2) ELISA (see the instruction model 2 on page XII(XXIV)). They are not controlled to rigorous specifications of prediluted antisera, which are adjusted to optimum
concentrations for RIA or other immunoassays.

Peptide Antisera
Code

Product / Description

14278-v

Adrenomedullin (Human) Antiserum(Rabbit)-20

Price:Yen
50 µl

Vial

32,0001) K. Hata, Y. Takebayashi, S. Akiba, R. Fujiwaki, K. Iida, K. Nakayama, S. Nakayama, M. Fukumoto,and K. Miyazaki, Mol. Hum. Reprod., 6, 867 (2000). (Immunohistochem.)
2) Y. Fujita, H. Mimata, N. Nasu, T. Nomura, Y. Nomura, and M. Nakagawa, Int. J. Urol., 9, 285 (2002).
(Immunohistochem.)
3) P. Rocchi, F., A.J. Zamora, X. Muracciole, E. Lechevallier, P.M. Martin, and L. Quafik, Cancer Res.,61, 1196 (2001). (RIA & Immunohistochem.)• This product is distributed under the license of Shionogi & Co., Ltd. Its use for any purpose otherthan research is strictly prohibited.

-20

Adrenomedullin 2 / Intermedin (Human, 1-7) Antiserum(Rabbit)
Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Adrenomedullin 2 (Human, 1-7)-TG
Adrenomedullin 2 / Intermedin (Human)
Adrenomedullin 2 / Intermedin (Rat)
Adrenomedullin (Human) Calcitonin(Human) CGRP (Human)

32,0001.8
1.6(TG: Bovine Thyroglobulin)

Reactivity:

50 µl

Vial

+
–
–
–
–

Absorbance at 415 nm

14438-v

1.4
1.2

Antiserum

1.0
0.8
0.6
0.4
0.2

Normal Serum

0.0
1000

10000

Serum Dilution

Adrenomedullin 2 (Human, 1-7) Antiserum Dilution Curve

1) K. Takahashi, K. Kikuchi, Y. Maruyama, T. Urabe, K. Nakajima, H. Sasano, Y. Imai, O. Murakami,
and K. Totsune, Peptides, 27, 1383 (2006).

– 261 –

Peptide Antisera andAdrenomedullin (Human) Antiserum Dilution Curve

Monoclonal Antibodies

Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Adrenomedullin (Human, 1-52)(Carrier-free)
Reactivity: Adrenomedullin (Human, 1-52)+
Adrenomedullin (Rat, 1-50)+
Adrenomedullin (Human, 1-25)–
Adrenomedullin (Human, 22-52)+
Adrenomedullin 2 / Intermedin (Human)–
Adrenomedullin 2 / Intermedin (Rat)–Peptide Antisera (continued)
Code

Product / Description

14220-v

Amylin (Rat) Antiserum(Rabbit)-20

Price:Yen
50 µl

Vial

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Amylin (Rat)-BSA(BSA: Bovine Serum Albumin)
Specificity: Amylin (Rat)100%
Amylin (Human)
60%
CGRP (Human)
0%
CGRP (Rat)0%
Calcitonin (Human)
0%
Sensitivity*: IC50
0.80 pmol/ml (Antiserum Dilution: x 20,000)****** Antisera of Amyloid β-Protein Related Peptides ******
14307-v-20

Amyloid β-Protein (Human, 1-40) Antiserum(Rabbit)

50 µl

Vial

25,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Amyloid β-Protein (Human, 1-40) (Carrier-free)Specificity: Amyloid β-Protein (Human, 1-40)
100%Amyloid β-Protein (Human, 1-25)100%Amyloid β-Protein (Human, 26-40)0%Amyloid β-Protein (Human, 17-25)0%Amyloid β-Protein (Human, 24-35)
0%Sensitivity*: IC50
0.60 pmol/ml (Antiserum Dilution: x 20,000)
No cross-reactions against the following peptides were observed at much higher doses (20-200 nmol/ml).
CRF (Human), BNP-32 (Human), Endothelin-1 (Human), Secretin (Human), and Substance P-20

Amyloid β-Protein (Human, 1-16) Antiserum
Amyloid β-Protein N-Terminal Specific Antiserum(Rabbit)
Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Amyloid β-Protein (Human, 1-16)-KLH(KLH: Keyhole Limpet Hemocyanin)
Reactivity: Amyloid β-Protein (Human, 1-16)
Amyloid β-Protein (Human, 1-40)
Amyloid β-Protein (Human, 1-42)
Amyloid β-Protein (Human, 1-43)

50 µl

Vial

25,0001++++

Absorbance at 415 nm

14359-v

0.75

Antiserum

0.5

0.25

Normal Serum
0
1000

10000

Serum Dilution

Amyloid β-Protein (Human, 1-16) Antiserum Dilution Curve-20

Amyloid β-Protein (Human, 34-40) Antiserum
Amyloid β-Protein (1-40) Specific Antiserum(Rabbit)
Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Amyloid β-Protein (Human, 34-40)-KLH(KLH: Keyhole Limpet Hemocyanin)
Reactivity: Amyloid β-Protein (Human, 1-40)+
Amyloid β-Protein (Human, 1-42)–
Amyloid β-Protein (Human, 1-16)–
Amyloid β-Protein (Human, 1-43)
–

50 µl

Vial

25,0001

Absorbance at 415 nm

14356-v

Antiserum
0.75

0.5

0.25

Normal Serum
0
1000

10000

Serum Dilution

Amyloid β-Protein (Human, 34-40) Antiserum Dilution Curve* All sensitivity data are measured by enzyme immunoassay using β-D-galactosidase as a label.

– 262 –

Peptide Antisera (continued)
Product / Description

14357-v

Amyloid β-Protein (Human, 37-42) Antiserum
Amyloid β-Protein (1-42) Specific Antiserum(Rabbit)-20

Price:Yen
50 µl

Vial

25,0001

Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Amyloid β-Protein (Human, 37-42)-KLH(KLH: Keyhole Limpet Hemocyanin)
Reactivity: Amyloid β-Protein (Human, 1-42)+
Amyloid β-Protein (Human, 1-40)–
Amyloid β-Protein (Human, 1-16)–

Absorbance at 415 nm

CodeAntiserum
0.75

0.5

0.25

Normal Serum
0
1000

10000

Serum Dilution

Amyloid β-Protein (Human, 37-42) Antiserum Dilution Curve

14414-v-20

Amyloid β-Protein (Human, 37-43) Antiserum
Amyloid β-Protein (1-43) Specific Antiserum(Rabbit)

Vial

50 µl

25,00014135-v-20

Vial

ANP (Human, 1-28) Antiserum
A-type(Atrial) Natriuretic Peptide (Human, 1-28)
Antiserum(Rabbit)

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: ANP (Human, 1-28)-TG(TG: Bovine Thyroglobulin)
Specificity: ANP (Human, 1-28)
100%
ANP (Human, 7-28)
100%[Met(O)12]-ANP (Human, 1-28)
150%
ANP (Rat, 1-28)
55%
β-ANP (Human, 1-28 Dimer)
100%
BNP-26 (Porcine)

Sensitivity*: IC50

0%0.28 pmol/ml (Antiserum Dilution: x 20,000)

No cross-reactions against the following peptides were observed at much higher doses (10-100 nmol/ml).
Oxytocin, [Arg8]-Vasopressin, Somatostatin, [Met5]-Enkephalin, and β-Endorphin (Human)

1) Y. Kasuya, T. Hirohama, and H. Uemura, Acta Histochem. Cytochem., 25, 363 (1992).
(Immunohistochem.)* All sensitivity data are measured by enzyme immunoassay using β-D-galactosidase as a label.

– 263 –

Peptide Antisera andAmyloid β-protein (Human, 37-43) Antiserum Dilution Curve

Monoclonal Antibodies

Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Amyloid β-Protein (Human, 37-43)-KLH(KLH: Keyhole Limpet Hemocyanin)
Reactivity: Amyloid β-Protein (Human, 1-43)+
Amyloid β-Protein (Human, 1-42)–
Amyloid β-Protein (Human, 1-40)–
Amyloid β-Protein (Human, 1-16)–

Peptide Antisera (continued)
Code

Product / Description

14200-v

BNP-26 (Porcine) AntiserumB-type(Brain) Natriuretic Peptide (Porcine, 1-26) Antiserum(Rabbit)-20

Price:Yen
Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: BNP-26 (Porcine)-TG (TG: Bovine Thyroglobulin)
Specificity: BNP-26 (Porcine)
100%
＜0.1%
BNP (Porcine, 20-26)＜0.1%
ANP (Human, 1-28)＜0.1%
ANP (Rat, 1-28)
Sensitivity*: IC50
5.5 pmol/ml (Antiserum Dilution: x 20,000)

1) Y. Kasuya, T. Hirohama, and H. Uemura, Acta Histochem. Cytochem., 25, 363 (1992).
(Immunohistochem.)

14051-v-20

Vial

Calcitonin (Human) Antiserum(Rabbit)

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Calcitonin (Human)-TG (TG: Bovine Thyroglobulin)
Specificity: Calcitonin (Human)
100%
Calcitonin (Human, 11-32)
100%
Calcitonin (Rat)100%
Calcitonin (Salmon)
0%
Sensitivity*: IC50
3.0 pmol/ml (Antiserum Dilution: x 100,000)
No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
CGRP (Human), Secretin (Human), Somatostatin, β-Endorphin (Human), and NPY (Porcine)

14176-v-20

Vial

CCK-33 (Porcine) Antiserum(Rabbit)

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: CCK-33 (Porcine)-TG(TG: Bovine Thyroglobulin)
100%
Specificity: CCK-33 (Porcine)
CCK-33 (Human)
60%
＜0.1%
Gastrin I (Human)＜0.1%
CCK-8 (Sulfated Form)＜0.1%
CCK-8 (Nonsulfated Form)＜0.1%
Peptide YY
Sensitivity*: IC50
0.3 pmol/ml (Antiserum Dilution: x 200,000)
No cross-reactions against the following peptides were observed at much higher doses (20-50 nmol/ml).
Glucagon (Human), NPY (Porcine, Bovine), Somatostatin, β-Endorphin (Human), Secretin (Human),and GRP (Human)

14160-v-20

CGRP (Human) Antiserum
Calcitonin Gene Related Peptide (Human) Antiserum(Rabbit)

Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: CGRP (Human)-TG (TG: Bovine Thyroglobulin)
Specificity: CGRP (Human)
100%
CGRP II (Human)
20%
CGRP (Rat)10%
Sensitivity*: IC50
0.45 pmol/ml (Antiserum Dilution: x 20,000)
No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
Methionin-Enkephalin, Oxytocin, NPY (Human), β-Endorphin (Human), and Somatostatin* All sensitivity data are measured by enzyme immunoassay using β-D-galactosidase as a label.

– 264 –

Peptide Antisera (continued)
Code

Product / Description

14233-v

CINC-1/gro (Rat) Antiserum
Cytokine-Induced Neutrophil Chemoattractant-1 /growth-related oncogene (Rat) Antiserum(Rabbit)-20

Price:Yen
Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: CINC/gro (Rat, 1-72)(Carrier-free)100%
Specificity: CINC/gro (Rat)[Cys(Acm)9,11]-CINC/gro (Rat, 1-17)
0%
CINC/gro (Rat, 11-34)
0%
CINC/gro (Rat, 35-50)
0%CINC/gro (Rat, 51-72)
0%Sensitivity*: IC50
0.19 pmol/ml (Antiserum Dilution: x 100,000)
No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
Glucagon (Human), Secretin (Human), Gastrin I (Human), CCK-8 (Sulfated Form), and Somatostatin

14250-v-20

CNP-22 (Human) AntiserumC-type Natriuretic Peptide-22 (Human) Antiserum(Rabbit)

Vial

50 µl

32,000No cross-reactions against the following peptides were observed at much higher doses (10-200 nmol/ml).
Secretin (Human), CRF (Human), Substance P, CGRP (Human), and Endothelin-2 (Human)14136-v-20

CRF (Human, Rat) Antiserum
Corticotropin Releasing Factor (Human, Rat) Antiserum(Rabbit)

Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: CRF (Human, Rat)-TG (TG: Bovine Thyroglobulin)
100%
Specificity: CRF (Human, Rat)
35%
CRF (Human, 32-41)＜0.1%
CRF (Ovine)＜0.1%
CRF (Ovine, 32-41)
Sensitivity*: IC50

5.2 pmol/ml (Antiserum Dilution: x 10,000)

No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
Oxytocin, [Arg8]-Vasopressin, [Met5]-Enkephalin, and Somatostatin* All sensitivity data are measured by enzyme immunoassay using β-D-galactosidase as a label.

– 265 –

Peptide Antisera andMonoclonal Antibodies

Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: CNP-22 (Human)-TG (TG: Bovine Thyroglobulin)
Specificity: CNP-22 (Human)
100%
CNP-53 (Porcine, Rat)100%
CNP-53 (Human)
100%
ANP (Human, 1-28)
0%
ANP (Rat, 1-28)
0%
BNP-32 (Human)0%
BNP-45 (Human)
0%0%BNP-32 (Rat)
Sensitivity*: IC50
0.25 pmol/ml (Antiserum Dilution: x 10,000)

Peptide Antisera (continued)
Product / Description

14338–v

β-Defensin-2 (Human) Antiserum(Rabbit)-20 Price:Yen
50 µl

Vial

Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: β-Defensin-2 (Human)-TG(TG: Bovine Thyroglobulin)
Reactivity: β-Defensin-2 (Human)+
β-Defensin-1 (Human)–
β-Defensin-3 (Human)–
β-Defensin-4 (Human)–
α-Defensin-1 (Human)–
α-Defensin-2 (Human)–
α-Defensin-3 (Human)–
α-Defensin-5 (Human)–

32,0001.6

Absorbance at 405 nm

CodeAntiserum

1.2

0.8

0.4

Normal Serum
0.0
1000

10000

Serum Dilutionb­Defensin­2 (Human) Antiserum Dilution Curve

1) Y. Abiko, A.K. Suraweera, M. Nishimura, T. Arakawa, T. Takuma, I. Mizoguchi, and T. Kaku,
Virchows Arch., 438, 248 (2001). (Immunohistochem.)
2) N. Mizukawa, K. Sawaki, H. Ngatsuka, M. Kamio, E. Yamachika, J. Fukunaga, T. Ueno, S. Takagi, andT. Sugahara, Anticancer Res., 21, 2171 (2001). (Immunohistochem.)
3) P.Y. Ong, T. Ohtake, C. Brandt, I. Strickland, M. Boguniewicz, T. Ganz, R.L. Gallo, and D.Y.M.
Leung, N. Engl. J. Med., 347, 1151 (2002). (Immunohistochem.)
4) P. Chadebech, D. D. Goidin, C. Jacquet, J. Viac, D. Schmitt, and M-J. Staquet, Cell Biol. Toxicol., 19,313 (2003). (Immunohistochem.)

14243-v-20

Vial

Elafin (Human) Antiserum(Rabbit)

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Elafin (Human, 1-57)(Carrier-free)Specificity: Elafin (Human)
100%
Sensitivity*: IC50
0.40 pmol/ml (Antiserum Dilution: x 20,000)
No cross-reactions against the following peptides were observed at much higher doses (10-250 nmol/ml).
Endothelin-1 (Human), ANP (Human, 1-28), CRF (Human), Substance P, and NPY (Human)* All sensitivity data are measured by enzyme immunoassay using β-D-galactosidase as a label.

– 266 –

Peptide Antisera (continued)****** Antisera of Endothelins ******
Code

Product / Description

14198-v

Endothelin-1 (Human) Antiserum(Rabbit)-20

Price:Yen
Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Endothelin-1 (Human, 1-21)-TG(TG: Bovine Thyroglobulin)
Specificity: Endothelin-1 (Human)
100%
Endothelin-2 (Human)
60%
Endothelin-3 (Human)
40%＜1%
Big Endothelin-1 (Human, 1-38)＜1%
Big Endothelin-1 (Porcine, 1-39)
Sensitivity*: IC50
1.0 pmol/ml (Antiserum Dilution: x 20,000)

1) S.H. Murch, C.P. Braegger, W.C. Sessa, and T.T. MacDonald, Lancet, 339, 381 (1992).
(Immunohistochem.)

Vial

50 µl

32,000

Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Big Endothelin-1 (Porcine, 22-39)-BSA(BSA: Bovine Serum Albumin)
Specificity: Big Endothelin-1 (Porcine, 22-39)
100%
Big Endothelin-1 (Porcine, 1-39)
100%
Big Endothelin-1 (Human, 1-38)
10%
Big Endothelin-1 (Human, 18-38)
10%Endothelin-1 (Human)
0%
Endothelin-2 (Human)
0%
Endothelin-3 (Human)
0%
Sensitivity*: IC50
0.45 pmol/ml (Antiserum Dilution: x 30,000)

14224-v-20

Big Endothelin-1 (Human, 22-38) Antiserum(Rabbit)
Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Big Endothelin-1 (Human, 22-38)-BSA(BSA: Bovine Serum Albumin)
Specificity: Big Endothelin-1 (Human, 1-38)
100%
Big Endothelin-1 (Human, 22-38)
50%
＜0.1%
Big Endothelin-2 (Human, 22-38)＜0.1%
Big Endothelin-3 (Human, 22-41 Amide)
Endothelin-1 (Human)
0%
Sensitivity*: IC50
2.5 pmol/ml (Antiserum Dilution: x 20,000)

No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
Peptide YY, CGRP (Human), Secretin (Human), β-Endorphin (Human), and Somatostatin* All sensitivity data are measured by enzyme immunoassay using β-D-galactosidase as a label.

– 267 –

Peptide Antisera and-20

Big Endothelin-1 (Porcine, 22-39) Antiserum(Rabbit)

Monoclonal Antibodies

14210-v

Peptide Antisera (continued)****** Antisera of Endothelins (continued) ******
Code

Product / Description

14225-v

Big Endothelin-2 (Human, 22-38) Antiserum(Rabbit)-20

Price:Yen
Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Big Endothelin-2 (Human, 22-38)-BSA(BSA: Bovine Serum Albumin)
Specificity: Big Endothelin-2 (Human, 1-38)
100%
Big Endothelin-2 (Human, 22-38)
200%
Big Endothelin-2 (Human, 1-37)
0%
Big Endothelin-1 (Human, 1-38)
0%
Big Endothelin-3 (Human, 1-41 Amide)
0%
Endothelin-1 (Human)
0%
Endothelin-2 (Human)
0%
Endothelin-3 (Human)
0%
Sensitivity*: IC50
0.25 pmol/ml (Antiserum Dilution: x 40,000)
No cross-reactions against the following peptides were observed at much higher doses (100-2,000 pmol/ml).
ANP (Human, 1-28), BNP-32 (Human), CNP-53 (Human), Substance P, and CRF (Human)

14238-v-20

Big Endothelin-2 (Human, 22-37) Antiserum(Rabbit)

Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Big Endothelin-2 (Human, 22-37)-BSA(BSA: Bovine Serum Albumin)
Specificity: Big Endothelin-2 (Human, 1-37)
100%
Big Endothelin-2 (Human, 1-38)
0%
Big Endothelin-2 (Human, 22-37)
400%
Big Endothelin-1 (Human, 1-38)
0%
Big Endothelin-3 (Human, 1-41 Amide)
0%
Endothelin-1 (Human)
0%
Endothelin-2 (Human)
0%
Endothelin-3 (Human)
0%
Sensitivity*: IC50 1.8 pmol/ml (Antiserum Dilution: x 100,000)
No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
Peptide YY, CGRP (Human), Secretin (Human), β-Endorphin (Human), and Somatostatin

14226-v-20

Big Endothelin-3 (Human, 22-41 Amide) Antiserum(Rabbit)

Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Big Endothelin-3 (Human, 22-41 Amide)-BSA(BSA: Bovine Serum Albumin)
Specificity: Big Endothelin-3 (Human, 1-41 Amide)
100%
Big Endothelin-3 (Human, 22-41 Amide)
35%
Big Endothelin-3 (Rat, 1-41 Amide)
100%
＜0.1%
Big Endothelin-1 (Rat, 1-39)
Big Endothelin-1 (Human, 1-38)
0%
Big Endothelin-2 (Human, 1-37)
0%Endothelin-1 (Human)
0%
Endothelin-2 (Human)
0%
Endothelin-3 (Human)
0%
Sensitivity*: IC50
0.45 pmol/ml (Antiserum Dilution: x 20,000)
No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
Peptide YY, CGRP (Human), Secretin (Human), β-Endorphin (Human), and Somatostatin* All sensitivity data are measured by enzyme immunoassay using β-D-galactosidase as a label.

– 268 –

Peptide Antisera (continued)
Code

Product / Description

14244-v

Galanin (Rat) Antiserum(Rabbit)-20

Price:Yen
Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Galanin (Rat)-BSA(BSA: Bovine Serum Albumin)
Sensitivity*: IC50

1.2 pmol/ml (Antiserum Dilution: x 30,000)

No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
Secretin (Human), CRF (Human), CGRP (Human), and ANP (Human, 1-28)

14127-v-20

GRF (Human) Antiserum
Growth Hormone Releasing Factor (Human) Antiserum(Rabbit)

Vial

50 µl

32,00014164-v-20

GRP (Human) Antiserum
Gastrin Releasing Peptide (Human) Antiserum(Rabbit)

Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: GRP (Human)-TG(TG: Bovine Thyroglobulin)
Specificity: GRP (Human)
100%
GRP (Porcine)75%
GRP (14-27)
55%
Neuromedin C (GRP, 18-27)
10%Bombesin10%＜0.1%
Neuromedin B＜0.1%
GRP (Human, 1-18)
Sensitivity*: IC50

0.34 pmol/ml (Antiserum Dilution: x 10,000)

No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
Glucagon (Human), Secretin (Human), Gastrin I (Human), Somatostatin, and CCK-8 (Sulfated Form)* All sensitivity data are measured by enzyme immunoassay using β-D-galactosidase as a label.

– 269 –

Peptide Antisera andNo cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
CRF (Human), VIP, NPY (Human), β-Endorphin (Human), and Somatostatin

Monoclonal Antibodies

Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: GRF (Human)-KLH(KLH: Keyhole Limpet Hemocyanin)
Specificity: GRF (Human)
100%
GRF (Porcine)100%
GRF (Human, 30-44 Amide)30%
GRF (Ovine)
5%
1%
GRF (Bovine)＜0.1%
GRF (Rat)＜0.1%
GRF (Human, 1-29)
Sensitivity*: IC50
0.35 pmol/ml (Antiserum Dilution: x 20,000)

Peptide Antisera (continued)
Code

Product / Description

14802-v

Lysenin Antiserum(Rabbit)-20

Price:Yen
50 µl

Vial

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Lysenin (Carrier-free)
Reactivity: Lysenin+
For Immunohistochemistry: x 100 - 1,000 dilution

Lysenin Antiserum Dilution Curve

-20

50 µl

Vial

Midkine (Human, 1-59) Antiserum(Rabbit)32,0001

Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Midkine (Human, 1-59)-TG(TG: Bovine Thyroglobulin)
Reactivity: Midkine (Human)+
Midkine (Human, 1-59)+
Midkine (Human, 60-121)–
Pleiotrophin (Human)–

Absorbance at 415 nm

14319-v

0.75

Antiserum
0.5

0.25

Normal Serum
0
1000

10000

Serum Dilution

Midkine (Human, 1-59) Antiserum Dilution Curve14158-v-20

NPY (Human, Rat) Antiserum
Neuropeptide Y (Human, Rat) Antiserum(Rabbit)

50 µl

Vial

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: NPY (Human)-TG (TG: Bovine Thyroglobulin)
Specificity: NPY (Human)
100%
NPY (Porcine)
100%
＜0.1%
NPY (Human, 1-9)
＜0.1%
NPY (Human, 24-36)＜0.1%
Pancreatic Polypeptide (Human)＜0.1%
Peptide YY
Sensitivity*: IC50
3.0 pmol/ml (Antiserum Dilution: x 50,000)
No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
Oxytocin, [Arg8]-Vasopressin, Somatostatin, ANP (Human, 1-28), and β-Endorphin (Human)

-20

50 µl

Vial

Orexin-A (Human) Antiserum(Rabbit)
Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Orexin A (Human)-BSA(BSA: Bovine Serum Albumin)
Reactivity: Orexin A (Human)++
Orexin B (Human)+
Orexin B (Rat)–
Glucagon-like Peptide 1 (Human, 7-36 Amide)

32,0001

Absorbance at 415 nm

14346-v

Antiserum
0.75

0.5

0.25

Normal Serum
0
1000

10000

Serum Dilution

Orexin-A (Human) Antiserum Dilution Curve* All sensitivity data are measured by enzyme immunoassay using β-D-galactosidase as a label.

– 270 –

Peptide Antisera (continued)
Code

Product / Description

14276-v

Osteocalcin (Human, 38-49) Antiserum(Rabbit)-20

Price:Yen
50 µl

Vial

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Osteocalcin (Human, 38-49)-BSA(BSA : Bovine Serum Albumin)
Specificity: Gla17,21,24-Osteocalcin (Human, 1-49)
100%
Glu17,Gla21,24-Osteocalcin (Human, 1-49)100%
Osteocalcin (Human, 38-49)200%
Osteocalcin (Human, 1-13)
0%
Sensitivity*: IC50
0.60 pmol/ml (Antiserum Dilution: x 50,000)
No cross-reactions against the following peptides were observed at much higher doses (20-200 pmol/ml).
Endothelin-1 (Human), ANP (Human, 1-28), CRF(Human), Substance P, and Secretin (Human)

14186-v-20

[Pyr33]-Pancreastatin (Porcine, 33-49) Antiserum(Rabbit)

50 µl

Vial

32,00014439-v-20

Vial

Proangiotensin-12 (Rat) Antibody(Rabbit)Affinity purified antibody
Lyophilized from 20 mM Phosphate Buffer Saline(pH 7.0) containing 5 mg Bovine Serum Albumin
Immunogen: Proangiotensin-12 (Rat)-TG(TG: Bovine Thyroglobulin)
Specificity: Proangiotensin-12 (Rat)
Proangiotensin-12 (Human)
Angiotensin I (Human)
Angiotensin II (Human)
Angiotensin III (Human)
Angiotensin IV (Human)
Angiotensin (Human, 1-7)
Angiotensin (Human, 1-14)
Angiotensin (Rat, 1-17)50 µg

50,00050 µl

32,000

+
–
–
–
–
–
–
–
–

1) S. Nagata, J. Kato, K. Sasaki, N. Minamino, T. Eto, and K. Kitamura,
Biochem. Biophys. Res. Commun., 350, 1026 (2006).

-20

Salusin-β (Human) Antiserum(Rabbit)Vial
1.8

Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Salusin-β (Human)-TG(TG: Bovine Thyroglobulin)
Reactivity: Salusin-β (Human)+
Salusin-α (Human)–

1.6

Absorbance at 415 nm

14418-v

1.4
1.2

Antiserum

1.0
0.8
0.6
0.4
0.2
0.0
1000

Normal Serum
10000

Serum Dilution

Salusin-β (Human) Antiserum Dilution Curve* All sensitivity data are measured by enzyme immunoassay using β-D-galactosidase as a label.

– 271 –

Peptide Antisera andNo cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
Peptide YY, CGRP (Human), Somatostatin, Secretin (Human), and β-Endorphin (Human)

Monoclonal Antibodies

Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: [Pyr33]-Pancreastatin (Porcine, 33-49)-TG(TG: Bovine Thyroglobulin)
Specificity: [Pyr33]-Pancreastatin (Porcine, 33-49)
100%
Pancreastatin (Porcine, 1-49)
100%
Chromogranin A (Human, 286-301 Amide) 100%
Sensitivity*: IC50
0.27 pmol/ml (Antiserum Dilution: x 50,000)

Peptide Antisera (continued)
Code

Product / Description

14112-v

Secretin (Porcine) Antiserum(Rabbit)-20

Price:Yen
Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Secretin (Porcine)-TG (TG: Bovine Thyroglobulin)
Specificity: Secretin (Porcine)100%
Secretin (Human)
100%
Secretin (Chicken)
0%
Sensitivity*: IC50
0.27 pmol/ml (Antiserum Dilution: x 50,000)
No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
GRP (Human), Gastrin I (Human), NPY (Porcine), Glucagon (Human), and Somatostatin

14216-v-20

SPAI-1 (Porcine) Antiserum
Sodium Potassium ATPase Inhibitor-1 (Porcine) Antiserum(Na+,K+-ATPase Inhibitor-1 Antiserum)(Rabbit)

Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: SPAI-1 (Porcine, 1-49)-BSA(BSA: Bovine Serum Albumin)
Specificity: SPAI-1 (Porcine, 1-49)
100%
SPAI-1 (Porcine, 1-14)
0%
SPAI-1 (Porcine, 25-35)
0%Ouabain0%
Sensitivity*: IC50
0.14 pmol/ml (Antiserum Dilution: x 30,000)
No cross-reactions against the following peptides were observed at much higher doses (100-200 pmol/ml).
Calcitonin (Human), CRF (Human), Endothelin-1 (Human), BNP-26 (Porcine), and Secretin (Human)

14365-v-20

Vial

Urotensin II (Human) Antiserum(Rabbit)
Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: Urotensin II (Human)-BSA(BSA: Bovine Serum Albumin)
Reactivity: Urotensin II (Human)++
Urotensin II (Rat)+Endothelin-1–
Somatostatin–++: Positive Color Reaction+: Weakly Positive Color Reaction–: Negative Color Reaction in 60 min

50 µl

32,000

Urotensin II (Human) Antiserum Dilution Curve

1) K. Takahashi, K. Totsune, O. Murakami, and S. Shibahara, Peptides, 22, 1175 (2001). (RIA)
2) K. Totsune, K. Takahashi, Z. Arihara, M. Sone, F. Satoh, S. Ito, Y. Kimura, H. Sasano,and O. Murakami, Lancet, 358, 810 (2001). (RIA)

14110-v-20

VIP (Human, Porcine) Antiserum
Vasoactive Intestinal Peptide (Human, Porcine) Antiserum(Rabbit)

Vial

50 µl

32,000Antiserum: lyophilized from 1 mM Phosphate Buffer (pH 7.0)
Immunogen: VIP (Human, Porcine)-TG (TG: Bovine Thyroglobulin)
100%
VIP (Human, Porcine)＜0.1%
Secretin (Human)＜0.1%
Glucagon (Human)＜0.1%
GRF (Human)
Sensitivity*: IC50
1.2 pmol/ml (Antiserum Dilution: x 10,000)

Specificity:

No cross-reactions against the following peptides were observed at much higher doses (1-10 nmol/ml).
CRF (Human), Oxytocin, NPY (Human), β-Endorphin (Human), and Somatostatin* All sensitivity data are measured by enzyme immunoassay using β-D-galactosidase as a label.

– 272 –