J. Inst. Electrostat. Jpn.

論静電気学会誌, 29, 1 (2005) 20-25

文ಽሶ⚵┙ߩ㍌ဳߣߒߡߩ 1 ᧄ㎮ DNA ߩિ㐳࿕ቯዊ ᨋ ℉ ਽ 1㧘㣐 ᵤ ᱜ ᄦ㧔2004 ᐕ 8 ᦬ 23 ᣣฃઃ, 2004 ᐕ 11 ᦬ 24 ᣣฃℂ㧕Stretching and Positioning of Single Stranded DNA
as a Template for Molecular Construction
Takuya KOBAYASHI1 and Masao WASHIZU((Received August 23,2004; Accepted November 24,2004)

The high-specificity self-assembling nature of DNA makes the molecule a candidate for the template for theconstruction of molecular devices. In order to construct a functional device, the template must be positioned onto apredetermined site on a substrate to allow external connections, and the components must be properly aligned onto thetemplate. A key factor is the high yield of binding, especially when the device consists of many components. Suchhigh yield requires that the bases of the template DNA be exposed so that its counterpart can interact freely, and thetemplate be stretched to avoid folding or coiling that hampers the interaction. For this purpose, we have developed amethod, by which a single-stranded DNA is stretched and immobilized bridging over an electrode pair, with themolecular ends anchored while the middle part is left free to interact with foreign molecules. We expect that thesestretch-and-positioned DNA with accessible base-pairs will lead to the high-yield molecular construction.
1.

ߪߓ߼ߦ࠻ࡠ࠾ࠢࠬ⚛ሶߩ㈩೉ߦࠃࠆ࿁〝᭴ᚑ߿㧘㔚ሶવ㆐♽╬ߩᯏ

DNA ߪ㧘4 ߟߩႮၮ㧔A,T,C,G㧕߇ 0.34nm ߩ㑆㓒ߢਗࠎߛ⢻ᕈಽሶߩ㈩೉ߦࠃࠆᯏ⢻ᕈಽሶ࡙࠾࠶࠻ߩ᭴▽ߥߤߩᔕ↪

᭴ㅧࠍᜬߜ㧘A ߪ T ߣ㧘G ߪ C ߣ․⇣⊛ߦ⚿วߔࠆᕈ⾰ࠍᜬ߇⠨߃ࠄࠇࠆ㧚DNA ߪ㧘ൻቇ⊛ߦ቟ቯߢ޽ࠅ㧘છᗧߩႮၮ㈩ߟ㧚೉ࠍኈᤃߦวᚑߢ߈ࠆߩߢ㧘ߎߩࠃ߁ߥಽሶ⚵┙ߩ㍌ဳ㧔࠹1)

Seeman ࠄ ߪ㧘ߎߩᕈ⾰ࠍ೑↪ߒߡ㧘․ቯߩ㈩೉ࠍᜬߟ
DNA ࠍวᚑߒ㧘⥄Ꮖ㓸วߦࠃࠅ࠽ࡁ᭴ㅧ૕ࠍ૞ᚑߔࠆᛛⴚࠍ㐿⊒ߒߚ㧚ߒ߆ߒߥ߇ࠄ㧘ߎߩࠃ߁ߥᚻᴺߦࠃࠅᓧࠄࠇࠆ࠽ࡦࡊ࡟࡯࠻㧕ߣߒߡᦨ߽߰ߐࠊߒ޿᧚ᢱߣ⠨߃ࠄࠇࠆ㧚਄ㅀߩࠃ߁ߥ⥄Ꮖ㓸วߦࠃࠆಽሶ⚵┙ࠍታ⃻ߔࠆߚ߼ߦߪ㧘ᰴߩ 2 ὐ߇ⷐ᳞ߐࠇࠆ㧚ࡁ᭴ㅧߪ㧘৻⥸ߦ෼₸߇ᭂ߼ߡૐߊ㧘߹ߚ㧘ߘߩ↥‛ߪṁᶧ1) ࠹ࡦࡊ࡟࡯࠻ DNA ߇㧘ၮ᧼਄ߩ੍߼ቯ߼ࠄࠇߚ૏⟎㧔ߚߣߒߡᓧࠄࠇࠆߚ߼㧘ᦸߺߩ૏⟎ߦಽሶࠍ⚵ߺ┙ߡࠆߣ޿ߞ

ߣ߃߫࿑ 1 ߩ႐วߦߪ 2 ߟߩ㔚ᭂ㑆㧕ߦ㧘ᄖ᧪ࠝ࡝ࠧ࠿ߚ↪ㅜߦߪਇะ߈ߢ޽ࠆ㧚 ৻ᣇ㧘DNA ࠍࡔ࠲࡜ࠗ࠯࡯࡚ࠪࠢ࡟ࠝ࠴࠼㧔એਅࡊࡠ࡯ࡉ DNA㧕߇⚿วߢ߈ࠆᒻߢ࿕ࡦߒߡ㈩✢⚛ሶߣߒߡ೑↪ߔࠆ࡮DNA ਄ߦන㔚ሶ࠻࡜ࡦࠫࠬቯߐࠇࠆߎߣ㧚࠲ࠍᒻᚑߔࠆߥߤߩࠕࠗ࠺ࠕ߇ឭ᩺ߐࠇߡ޿ࠆ߇2-3)㧘ߎࠇࠄߦ߅޿ߡ߽㧘DNA ࠍ․ቯߩ૏⟎ߦ㈩೉ߔࠆߣ޿߁ၮᧄᛛⴚ߇
ᧂ⸃᳿ߩ߹߹ᱷߐࠇߡ޿ࠆ㧚2) ࠹ࡦࡊ࡟࡯࠻ DNA ߣࡊࡠ࡯ࡉ DNA ߇㜞޿෼₸ߢ⚿วߢ߈ࠆߎߣ㧚㧝ߟߩࡊࡠ࡯ࡉ DNA ߇࠹ࡦࡊ࡟࡯࠻ DNA ߣ⚿วߔࠆ⏕߽ߒ㧘࿑ 1 ߦ␜ߔࠃ߁ߦ㧘DNA ࠍ࿕ቯߒߡ߅߈㧘ߎߎߦࠝ₸ࠍKߣߔࠆߣ,N ୘ߩ⚛ሶ߇⚿วߔࠆ⏕₸ߪK N㧩K N ߢ޽ࠆ㧚࡝ࠧ࠿ࠢ࡟ࠝ࠴࠼㧔⍴޿ 1 ᧄ㎮ DNA㧕ߢ࡜ࡌ࡞ߒߚಽሶࠍ⚿㕖⃻ታ⊛ߦ㜞޿K㧩90㧑ߣ޿߁઒ቯߩ߽ߣߢ߽㧘10 ୘ߩ⚛ሶวߐߖࠇ߫㧘છᗧߩಽሶࠍ㧘છᗧߩ㗅ᐨߢ㧘0.34 nm ߩಽ⸃⢻ߢ㧘ၮ᧼਄ߩ․ቯߩ૏⟎ߦ㈩೉ߔࠆߎߣ߇ߢ߈ࠆߢ޽ࠈ߁
4)㧚ߎߩࠃ߁ߥᛛⴚߦߪ㧘࿑ 1 ߦ଀ߣߒߡឬ޿ߚಽሶࠛ࡟ࠢࠠ࡯ࡢ࡯࠼㧦DNA, template, self-assembly, electrostatic field᧲੩ᄢቇᎿቇ♽⎇ⓥ⑼ᯏ᪾Ꮏቇኾ᡹㧔113-8656 ᧲੩ㇺᢥ੩඙ᧄㇹ 7-3-1㧕
Department of Mechanical Engineering, The University ofTokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan㧚
1kobayashi@washizu.t.u-tokyo.ac.jp

࿑ 1 DNA 䉕㍌ဳ䈫䈚䈢ಽሶ⚵┙䈱᭎ᔨ࿑
Fig.1 Conceptual sketch of DNA-based molecularconstruction.

分子組立の鋳型としての 1 本鎖 DNA の伸長固定（小林・鷲津）21ಽሶ⚵┙ߩ㍌ဳߣߒߡߩ 1 ᧄ㎮ DNA ߩિ㐳࿕ቯ㧔ዊᨋࠄ㧕߆ࠄߥࠆࠪࠬ࠹ࡓ߇᭴▽ߐࠇࠆ⏕₸ߪ 0.3, 100 ୘ߩ⚛ሶߥ
ࠄ 0.00002 ߦߒ߆ߥࠄߥ޿㧚ࠝ࡝ࠧ࠿ࠢ࡟ࠝ࠴࠼ߩ DNA ߳ߩ⚿วࠍ೑↪ߔࠆᓥ᧪ᛛⴚ
ߣߒߡߪ㧘FISH㧔Fluorescence In Situ Hybridization)߇޽ࠆ㧚ߎ
ߩᚻᴺߪ㧘ᄌᕈߐߖߡ 2 ᧄ㎮ࠍ㐿ⵚߒߚࠥࡁࡓ DNA ߦⰯశ࡜ࡌ࡞ߒߚࠝ࡝ࠧ࠿ࠢ࡟ࠝ࠴࠼ࠍ⚿วߐߖ㧘㗼ᓸ㏜ਅߢ⋥ធⷰኤߔࠆߎߣߦࠃࠅ․ቯ㈩೉ߩ૏⟎ࠍᬌ಴ߔࠆ߽ߩߢ޽ࠆ㧚ߒ߆ߒߥ߇ࠄ㧘ߎߩࠃ߁ߥᚻᴺߦ߅޿ߡߪ㧘2 ᧄ㎮߇㐿ⵚߒߡࠝ࡝ࠧ࠿ࠢ࡟ࠝ࠴࠼߇⚿วߢ߈ࠆࠃ߁ߦႮၮ߇㔺಴ߔࠆ⏕₸ߪૐߊ㧘߆ߟ㧘ࠝ࡝ࠧ࠿ࠢ࡟ࠝ࠴࠼߇⚿วߒߚߣߒߡ߽㧘㐿ⵚߒߚ DNA ߇రߩ 2 ᧄ㎮ߦᚯࠆࡊࡠ࠮ࠬߣߩ┹วߦߥࠆ㧚ᓥߞߡ㧘2 ᧄ㎮ DNA ࠍߘߩ߹߹࠹ࡦࡊ࡟࡯࠻ߣߒߡ↪޿ࠆߩ

࿑ 2㩷 ੑᧄ㎮ DNA 䈱㕒㔚િ㐳࿕ቯߢߪ㧘ᧄ⾰⊛ߦ㜞޿෼₸ࠍᓧࠆߎߣߪߢ߈ߥ޿㧚Fig.2 Electrostatic stretch-and-positioning ofdouble-stranded DNA.

ߎߩⷰὐ߆ࠄߪ㧘Ⴎၮ߇ኻࠍᒻᚑߒߡ޿ߥ޿ 1 ᧄ㎮ DNA߇࠹ࡦࡊ࡟࡯࠻ߣߒߡᦸ߹ߒ޿ߣ⠨߃ࠄࠇࠆ㧚ߒ߆ߒߥ߇ࠄ㧘৻⥸ߦ 1 ᧄ㎮ DNA ߪ persistent length5)߇⍴ߊ㧘ࠦࠗ࡞⁁ߩᒻ

3.

1 ᧄ㎮ DNA ߩⰯశᮡ⼂ᘒࠍขࠅ߿ߔ޿㧚߽ߒࠦࠗ࡞⁁ߦਣ߹ߞߡߒ߹߁ߣ㧘ᄖ᧪ಽ2 ᧄ㎮ DNA ߩಽᭂߪ㧘DNA ߩ࡝ࡦ㉄ၮߦᒁ߈ነߖࠄࠇߚ

ሶ߇Ⴎၮߦࠕࠢ࠮ࠬߒߦߊߊߥࠆ਄ߦ㧘㎮ౝߢኻวࠍ૞ࠆߥ

ࠞ࠙ࡦ࠲࡯ࠗࠝࡦߩ⒖േߦࠃࠅ↢ߕࠆ㧚ᓥߞߡ㧘1 ᧄ㎮ DNAߤߒߡ㧘ࡊࡠ࡯ࡉ DNA ߩ⚿วࠍᅹኂߔࠆߎߣߦߥࠆ㧚ᓥߞߦ߅޿ߡ߽㕒㔚િ㐳߇น⢻ߢ޽ࠆߣ⠨߃ࠄࠇࠆ㧚ߒ߆ߒߥ߇ߡ㧘࠹ࡦࡊ࡟࡯࠻ DNA ߪ⋥✢⁁ߦᒁ߈િ߫ߐࠇߚᒻߢ޽ࠆ

ࠄ㧘2 ᧄ㎮ DNA ߪႮၮኻߩߚ߼ߦᲧセ⊛ᦛ߇ࠅߦߊ޿᭴ㅧࠍߎߣ߇᳞߼ࠄࠇࠆ㧚⋥✢⁁ߩᒻᘒߪ㧘ߐࠄߦ㧘⋥✢਄ߩᒻᘒᜬߟߩߦኻߒ㧘㧝ᧄ㎮ DNA ߪ㧘⢛㛽๟ࠅߩ࿁ォ⥄↱ᐲ߇޽ߦߪ㧘a) ಽሶ㈩೉ߩ⹏ଔ߇ߒ߿ߔ޿㧘b) ߚߣ߃߫࿑ 1 ߩࠃࠅ㧘㎮ౝႮၮኻว߿᛬ࠅ⇥ߺࠍᒻᚑߒ߿ߔ޿ߚ߼㧘ࠦࠗ࡞⁁߁ߥಽሶ⚛ሶ࿁〝ߩ႐วߦߪ࿁〝ߩ⍴⛊ࠍ㒐ߋ㧘ߣ޿ߞߚ೑ߩᒻᘒࠍขࠅ߿ߔ޿㧚ᓥߞߡ㧘1 ᧄ㎮ DNA ߩિ㐳ߦߪ㧘2 ᧄὐ߽޽ࠆ㧚㎮ DNA ࠃࠅ߽㜞޿㔚⇇ᒝᐲ߇ᔅⷐߢ޽ࠆߎߣ߇੍ᗐߐࠇࠆ㧚ߎࠇࠄߩὐߦ㐓ߺ㧘੹࿁ߩ⎇ⓥߢߪ㧘1 ᧄ㎮ DNA ࠍ⋥ធિ1 ᧄ㎮ DNA ߩ㕒㔚િ㐳ߩⷰኤࠍⴕ߁㓙ߩ 1 ߟߩᛛⴚ⊛໧㗴㐳࿕ቯߔࠆᣇᴺࠍ㐿⊒ߒ㧘ߎߎ߳ߩࠝ࡝ࠧ࠿ࠢ࡟ࠝ࠴࠼ߩ⚿ߪ㧘2 ᧄ㎮ DNA ߩ႐วߩࠗࡦ࠲࡯ࠞ࡟࡯࠲⦡⚛ߩࠃ߁ߥ㧘1วߩⷰኤࠍ⹜ߺߚߩߢ㧘ߎߎߦႎ๔ߔࠆ㧚ಽሶⷰኤࠍน⢻ߦߔࠆ߶ߤචಽߥⰯశᒝᐲࠍᜬߟ⦡⚛߇ή޿ߎߣߦ޽ࠆ㧚ߘߎߢ㧘߹ߕ㧘1 ᧄ㎮ DNA ߩⰯశᮡ⼂ᴺߩ㐿⊒2.

2 ᧄ㎮ DNA ߩ㕒㔚િ㐳࿕ቯࠍⴕߞߚ㧚
ߘߩᚻᴺࠍ࿑ 3 ߦ␜ߔ㧚ㆡᒰߥࡊ࡜ࠗࡑ࡯ࠍ↪޿㧘ᚒ‫⎇ߩޘ‬ⓥቶߢߪ 2 ᧄ㎮ DNA ߩિ㐳࿕ቯᛛⴚࠍߔߢߦ㐿2 ᧄ㎮ DNA ߩ․ቯߩ㗔ၞࠍ PCR ߦࠃࠅჇ᏷ߔࠆ㧚ߎߩ PCR⊒ߒߡ޿ࠆ 6)㧚ߘߩේℂࠍ࿑ 2 ߦ␜ߔ㧚

ߩㆊ⒟ߦ߅޿ߡ,Ⱟశᮡ⼂ߐࠇߚࡕࡁࡑ࡯㧔Alexa dUTP㧕ࠍᷙ

2 ᧄ㎮ DNA ߪ 2nm ߩ⋥ᓘ㧘 3kbp ޽ߚࠅ⚂ 1Pm ߩ㐳ߐࠍ౉ߒߡ߅ߌ߫㧘Ⱟశ߇ DNA ㎮ߦ⚵ߺㄟ߹ࠇࠆ㧚ߒ߆ߒߥ߇ᜬߟ㧚ߎߩࠃ߁ߥ㐳޿߽߭⁁ಽሶߪ᳓ṁᶧਛߢߪᾲㆇേߦࠃࠄ,ߎߩࡊࡠ࠮ࠬߦࠃࠅᓧࠄࠇࠆ߽ߩߪ㧘੕޿ߦ⋧⵬⊛ߥ 1 ᧄࠅ࡜ࡦ࠳ࡓࠦࠗ࡞⁁ߦߥߞߡ޿ࠆ㧚ᓸ⚦ടᎿߦࠃࠅᒻᚑߐࠇ

㎮ DNA ߩ 50%ߕߟߩᷙว‛ߢ޽ࠆߩߢ㧘޽ࠆ⏕₸ߢ੕޿ߦߚ㔚ᭂࠍᜬߟၮ᧼਄ߦ DNA ᳓ṁᶧࠍ⟎߈,1MHz 1MV/m ߩ㜞⚿วߒߡ 2 ᧄ㎮ DNA ߦᚯߞߡߒ߹߁ߎߣߦߥࠆ㧚ᓥߞߡ㧘๟ᵄ࡮㜞㔚⇇ࠍශടߔࠆߣ,DNA ߪಽᭂߒ㧘ᄖㇱ㔚⇇ߣߩ⋧⚐☴ߥ 1 ᧄ㎮ DNA ߩ㕒㔚િ㐳ࠍⷰኤߔࠆߚ߼ߦߪ㧘ߎߎߢ੕૞↪ߦࠃࠅ⋥✢⁁ߦᒁ߈િ߫ߐࠇࠆ㧚ߎߩᤨ㧘㔚ᭂߪෘߐᓧࠄࠇߚ 2 ߟߩ 1 ᧄ㎮ DNA ߩ߁ߜߩ޿ߕࠇ߆ࠍቢోߦขࠅ

ᢙච nm ߣ⭯ߊ㧘㔚ᭂࠛ࠶ࠫߦᒝ޿㔚⇇㓸ਛ߇↢ߓߡ޿ࠆߩ㒰ߊᔅⷐ߇޽ࠆ㧚ߘߎߢ㧘ߎߎߢߪ㧘ࠕࡆࠫࡦ-ࡆࠝ࠴ࡦ⚿ว

ߢ㧘DNA ߪ㧘⺃㔚ᵒേߦࠃࠅ㧘㔚ᭂࠛ࠶ࠫߦಽሶ┵߇ធߔࠆࠍ↪޿ߡߎߩ㒰෰ࠍⴕߞߚ㧚ߔߥࠊߜ㧘࿑ 3 a)ߦ␜ߔࠃ߁ߦ㧘߹ߢᒁ߈ነߖࠄࠇࠆ㧚ࠕ࡞ࡒ࠾࠙ࡓߩࠃ߁ߦ㔚᳇ൻቇ⊛ߦᵴ

PCR ࠍⴕ߁㓙ߩࡊ࡜ࠗࡑ࡯ߩ৻ᣇߣߒߡ㧘5'┵ߦࡆࠝ࠴ࡦࠍᕈߥ㊄ዻࠍ㔚ᭂ᧚ᢱߣߒߡ↪޿ߚ႐วߦߪ㧘㔚ᭂߣធ⸅ߒߚᜬߟ߽ߩࠍ↪޿ࠆ㧚PCR ࠍⴕ޿㧔࿑ 3 b㧕㧘ᓧࠄࠇߚ DNA ࠍಽሶ┵ߪ㔚ᭂߦ᳗ਭ⊛ߦ࿕ቯߐࠇࠆߎߣߦߥࠆ㧚ࠕࡆࠫࡦ࿕ቯൻߒߚ⏛᳇ࡆ࡯࠭ߦ࿕ቯߔࠆ㧔࿑ 3 c㧕㧚਄Ẵߺ

- 2 -22

静電気学会誌

第 29 巻

第 1 号（2005）

࿑ 4 㔚⇇ᒝᐲ䋭િ㐳㐳䈘䈱㑐ଥ(a)㩷 ੑᧄ㎮ DNA, (b)㩷 ৻ᧄ㎮ DNA
Fig.4 Field strength v.s. stretched length for(a) double-stranded (ds-) and(b) single-stranded (ss-) DNA.

ߥ߅㧘ߎߩᣇᴺߦࠃࠅᓧࠄࠇࠆⰯశᮡ⼂ DNA ߩࠨࠗ࠭ߩ਄㒢ߪ㧘PCR ߩ෼₸ߦࠃࠅ᳿߹ࠅ,ᦨᄢߢ⚂ 10kbp ߢ޽ߞߚ㧚4.

࿑ 3㩷 ৻ᧄ㎮ DNA 䈱Ⱟశᮡ⼂1 ᧄ㎮ DNA ߩ㕒㔚િ㐳ߎߩࠃ߁ߦߒߡᓧࠄࠇߚⰯశᮡ⼂ 1 ᧄ㎮ DNA ࠍ↪޿ߡ㧘

Fig.3 Fluorescence labeling of single-stranded DNA.

㕒㔚㈩ะߩⷰኤࠍⴕߞߚ㧚৻⥸ߦ㧘⭯⤑㔚ᭂࠍ㔚Ḯߦធ⛯ߒߦᱷࠆᧂ⚿ว DNA ࠍᵞ޿ᵹߒߚᓟ㧘0.1M ߩ NaOH ߢᄌᕈߐߡ㔚࿶ࠍශടߔࠆߣ㧘㔚ᭂࠛ࠶ࠫው┵ߦ߅޿ߡ EHD ኻᵹ߇ߖ㧘⸃㔌ߒߡ߈ߚ 1 ᧄ㎮ DNA ࠍ࿁෼ߔࠆ㧔࿑ 3 d㧕㧚↢ߓࠆ߇㧘ߎߩࠃ߁ߥኻᵹ߇޽ࠆߣ㧘DNA ߩિ㐳߇㕒㔚િ㐳ߎߩࡊࡠ࠮ࠬߦࠃࠅᓧࠄࠇߚ 1 ᧄ㎮ DNA ߩ⚐ᐲࠍ㧘1 ᧄ㎮ߦࠃࠆ߽ߩ߆ᵹ૕ജቇ⊛િ㐳ߦࠃࠆ߽ߩ߆߇ࠊ߆ࠄߥߊߥߞ

DNA ߩߺࠍᶖൻߔࠆ Mung Bean nuclease (TAKARA) ࠍ↪޿ߡߒ߹߁㧚ߘߎߢ㧘એਅߩታ㛎ߢߪ㧘ኻᵹߩᓇ㗀ࠍฃߌߦߊ

ߡ᷹ቯߒߚ㧚1 ᧄ㎮ DNA ࠍ Mung Bean ߢᶖൻ㧔5 Unit, 23 ᐲ,޿ࡈࡠ࡯࠹ࠖࡦࠣࡐ࠹ࡦࠪࡖ࡞㔚ᭂ 7)ࠍ↪޿ߡ㧘ታ㛎ࠍⴕߞ

10 ಽ㧕ߒߚ႐ว,1 ᧄ㎮ DNA ߪቢోߦᶖൻߐࠇ,2 ᧄ㎮ DNAߚ㧚ߪᬌ಴㒢⇇એਅߒ߆฽߹ࠇߡ޿ߥ޿ߎߣ߇⏕⹺ߐࠇߚ㧚Ყセ࿑ 4 ߪ㧘㔚ᭂࠛ࠶ࠫߦ ┵࿕ቯߐࠇߚ DNA ߩ㧘㔚⇇ᒝᐲߩߚ߼ߦ 2 ᧄ㎮ DNA ߦኻߒߡ߽ห᧦ઙਅߢหߓታ㛎ࠍߒߚߦኻߔࠆિ㐳㐳ߐߩ᷹ቯ⚿ᨐࠍ㧘2 ᧄ㎮ DNA (a)ߣ 1 ᧄ㎮ DNAߣߎࠈ,2 ᧄ㎮ DNA ߪᶖൻߐࠇߥ޿ߎߣ߇⏕⹺ߐࠇߚ㧚ᓥߞ(b)ߦߟ޿ߡࡊࡠ࠶࠻ߒߚ߽ߩߢ޽ࠆ㧚DNA ߪ޿ߕࠇ߽ 10kbߡ㧘࿑ 3 ߩᚻᴺߦࠃࠅ㧘นⷞൻⷰኤࠍⴕ߁ߩߦචಽߥ⚐ᐲߢߢ޽ࠆ㧚 ࿑ਛߢ㧘⍫ශߪ㧘㔚࿶ࠍჇട࡮ᷫዋߐߖߚᤨߩᤨ㑆1 ᧄ㎮ DNA ߇ᓧࠄࠇߚߣ⠨߃ࠄࠇࠆ㧚⚻ㆊࠍ␜ߒߡ޿ࠆ㧚- 3 -分子組立の鋳型としての 1 本鎖 DNA の伸長固定（小林・鷲津）23ಽሶ⚵┙ߩ㍌ဳߣߒߡߩ 1 ᧄ㎮ DNA ߩિ㐳࿕ቯ㧔ዊᨋࠄ㧕6.

࿑ 4 a)ߩ 2 ᧄ㎮ DNA ߩ႐ว,㔚⇇ᒝᐲ E ࠍჇടߐߖࠆߦᓥ1 ᧄ㎮߳ߩࡂࠗࡉ࡝࠳ࠗ࠯࡯࡚ࠪࡦ

ߞߡ㧘િ㐳㐳ߐߪჇടߒ㧘⚂ 3.4Pm ߢ㘻๺ߒߚ㧚ߎࠇߪ 10kbp

ߎߩࠃ߁ߦિ㐳࿕ቯߐࠇߚ 1 ᧄ㎮࠹ࡦࡊ࡟࡯࠻ DNA ߦኻ㧔DNA ߩႮၮߩ㑆㓒 0.34 nm×10kb㧕ߩ 2 ᧄ㎮ DNA ߩℂ⺰㐳ߐߔࠆ 1 ᧄ㎮ࡊࡠ࡯ࡉ DNA ߩ⚿วߩേᘒⷰኤࠍ⹜ߺߚ㧚ߎߩߣ৻⥌ߔࠆ㧚E ࠍᷫዋߐߖߡ޿ߊߣ㧘િ㐳㐳ߐߪ㧘E ࠍჇടታ㛎ߢߪ㧘࠹ࡦࡊ࡟࡯࠻ DNA ߣࡊࡠ࡯ࡉ DNA ࠍ೎୘ߦⷰኤߐߖߡ޿ߞߚᤨߩᦛ✢ࠍㅒߦߚߤߞߡዊߐߊߥߞߚ㧚ߥ߅㧘ߔࠆߚ߼㧘ਔ⠪ࠍ⇣ߥࠆⰯశ⦡⚛ߢ࡜ࡌ࡞ߒߚ㧚ߔߥࠊߜ㧘E = 0 ߢ㐳ߐ߇ 0 ߦߥࠄߥ޿ߩߪ㧘శቇ᷹ቯߩಽ⸃⢻߇ 0.5Pma) ࠹ࡦࡊ࡟࡯࠻㧦10kb ߩ 1 ᧄ㎮ DNA㧚Alexa488㧔B ബ⿠㧘ബ⿠ᵄ㐳 490nm㧘Ⱟశᵄ㐳 520nm㧕ߢ࡜ࡌ࡞㧚⒟ᐲߒ߆ߥ޿߆ࠄߢ޽ࠆ㧚৻ᣇ㧘࿑ 4 b)ߩ 1 ᧄ㎮ DNA ߩ႐วߦߪ,િ㐳ߔࠆߦߪࠃࠅb) ࡊࡠ࡯ࡉ DNA㧦࠹ࡦࡊ࡟࡯࠻ DNA ߩਛᄩઃㄭߣ⋧⵬ᄢ߈ߥ㔚⇇ᒝᐲ߇ᔅⷐߢ޽ࠆ߇㧘િ߮ߚ⁁ᘒࠍ଻ߟߦߪࠃࠅ

⊛ߥ㈩೉ࠍᜬߟ㧘5kb ߩ 1 ᧄ㎮ DNA㧚Alexa564 (G ബ⿠㧘ዋߥ޿㔚⇇ᒝᐲߢߔ߻ߣ޿߁㧘᣿⏕ߥࡅࠬ࠹࡝ࠪࠬࠍ␜ߒߚ㧚ബ⿠ᵄ㐳 575nm㧘Ⱟశᵄ㐳 600nm)ߢ࡜ࡌ࡞㧚ࠍ࿑ 3 ߩᣇᴺࠍ↪޿ߡ૞ߞߚ㧚ߎࠇߪ㧘1 ᧄ㎮ DNA ߦ߅޿ߡߪ㧘ㇱಽ⊛ߥ㎮ౝႮၮኻวߩᒻታ㛎ᚻ㗅ߪએਅߢ㧘᷷ᐲ᧦ઙ߇․ߦ⸥ߐࠇߡ޿ߥ޿㒢ࠅቶᚑ޽ࠆ޿ߪ⇹᳓ᕈ⋧੕૞↪ߥߤߩ㎮ౝߩ⋧੕૞↪߇ᄢ߈ߊ㧘᷷ߢⴕߥߞߚ㧚㔚⇇ᒝᐲࠍ਄ߍߡ޿ߊᤨߦߪ㧘ߎࠇࠄߩ⋧੕૞↪ࠍᒁ߈㔌ߔߚ߼ߦࠃࠅᄢ߈ߥജ߇ᔅⷐߢ޽ࠆߎߣߦ⿠࿃ߒߡ޿ࠆ߽ߩߣi) ࠹ࡦࡊ࡟࡯࠻ DNA ࠍቢోߦ⸃㔌ߐߖࠆߚ߼㧘94 ᐲߢ 2⠨߃ࠄࠇࠆ㧚ߎߩ႐วߦ߽ E ߇ᄢ߈޿ߣߎࠈߢિ㐳㐳ߐ߇㘻ಽ,0 ᐲߢ 2 ಽࠗࡦࠠࡘࡌ࡯࠻ߒߚᓟ,2Pm ߩࠡࡖ࠶ࡊࠍ๺ߔࠆ߇㧘ߎߩ㐳ߐߪ㧘2 ᧄ㎮ DNA ߩ⚂ 2/3 ⒟ᐲߢ޽ߞߚ㧚ᜬߟࡈࡠ࡯࠹ࠖࡦࠣࡐ࠹ࡦࠪࡖ࡞㔚ᭂߦਔ┵࿕ቯ㧚ii) ࡊࡠ࡯ࡉ DNA ߩๆ⌕㒐ᱛߩߚ߼ߦ Bovine serum5.

1 ᧄ㎮ DNA ߩિ㐳࿕ቯalbumin (10mg/mL)ࠍዉ౉㧘ࠟ࡜ࠬ⴫㕙ࠍⷒ߁㧚iii) ࡊࡠ࡯ࡉ DNA ࠍዉ౉ߒࡂࠗࡉ࡝࠳ࠗ࠭ߐߖࠆ㧚ߎߩࠃ߁ߦિ㐳ߒߚ 1 ᧄ㎮ DNA ࠍಽሶ⚵┙ߩߚ߼ߩ࠹ࡦ
ࡊ࡟࡯࠻ߣߒߡ↪޿ࠆߚ߼ߦߪ㧘㔚⇇ࠍขࠅ෰ߞߚᓟߢ߽

࿑ 6 ߪ⚐᳓ਛߢ࠹ࡦࡊ࡟࡯࠻ DNA ߣࡊࡠ࡯ࡉ DNA ߩࡂࠗDNA ߇િ㐳ߐࠇߚ߹߹ߢߥߌࠇ߫ߥࠄߥ޿㧚߹ߚ㧘િ㐳ߐࠇࡉ࡝࠳ࠗ࠯࡯࡚ࠪࡦࠍ⹜ߺߚ⚿ᨐߢ㧘(a)߇ B ബ⿠ߢ⷗ߚ࠹ࡦ

ߚ DNA ߇㔚ᭂ㑆ߩၮ᧼ߦๆ⌕ߒߡߒ߹߁ߣ㧘ᄖ᧪ಽሶ߇⚿ࡊ࡟࡯࠻ DNA㧘(b)߇ G ബ⿠ߢ⷗ߚࡊࡠ࡯ࡉ DNA ߢ޽ࠆ㧚วߢ߈ߥߊߥࠆ㧚ߎߩࠃ߁ߥ᧦ઙࠍḩߚߔ࿕ቯࠍታ⃻ߔࠆ߽࿑ a)ߣ b)ߢߪ㧘ࠬ࠹࡯ࠫࠍേ߆ߐߕߦബ⿠శࠍಾࠅᦧ߃ࠆߎߩߣߒߡߪ㧘ࡈࡠ࡯࠹ࠖࡦࠣࡐ࠹ࡦࠪࡖ࡞㔚ᭂ♽߇㐿⊒ߐࠇߣߦࠃࠅ㧘หߓ૏⟎ࠍ⷗ߡ޿ࠆ߇㧘a)ߩ࠹ࡦࡊ࡟࡯࠻ DNA ߇7)ߡ޿ࠆ 㧚࿑ 5 ߪ㧘ߎߩ㔚ᭂ♽ࠍ↪޿ߡⴕߞߚ 1 ᧄ㎮ DNA ߩ⷗߃ߡ޿ࠆ૏⟎ߦߪ㧘b)ߩࡊࡠ࡯ࡉ DNA ߪోߊ⷗߃ߡ޿ߥિ㐳࿕ቯߩ౮⌀ߢ㧘ࠡࡖ࠶ࡊ㐳 2Pm ߩ㔚ᭂࠍᯅ⛊ߔࠆᒻߢ޿㧚 ߔߥࠊߜ㧘࠹ࡦࡊ࡟࡯࠻ߣࡊࡠ࡯ࡉߩࡂࠗࡉ࡝࠳ࠗ࠯࡯

ᄙᢙߩ 1 ᧄ㎮ DNA ߇࿕ቯߐࠇߡ޿ࠆߩ߇⷗߃ࠆ㧚ߎࠇࠄߩ࡚ࠪࡦߪ↢ߓߥ߆ߞߚ߽ߩߣᕁࠊࠇࠆ㧚࿕ቯߐࠇߚ DNA ߪ㧘ṁᶧߩᵹࠇߦࠃߞߡḧᦛߔࠆ᭽ሶ߇ⷰ

ߎߩේ࿃ߪ㧘DNA ߩ⢛㛽߇࡝ࡦ㉄ၮߩߚ߼ߦ⽶ߦᏪ㔚ߒߡኤߐࠇߚߎߣ߆ࠄ㧘DNA ߪၮ᧼ߦๆ⌕ߐࠇߡ޿ߥ޿ߎߣ߇⏕޿ࠆߚ߼,㕒㔚෻⊒ജߦࠃࠅࡊࡠ࡯ࡉ DNA ߇࠹ࡦࡊ࡟࡯࠻⹺ߐࠇߚ㧚DNA ߦㄭߠߌߥ߆ߞߚߎߣߦ޽ࠆߣ⠨߃㧘ṁᶧߩႮỚᐲࠍ਄ߍߡ㧘ߎߩ㕒㔚⋧੕૞↪ࠍᒙ߼ࠆߎߣߣߒߚ㧚૶↪ߒߚࡃ࠶ࡈࠔ࡯ߪ Tris-HCl (pH 7.0), 100 mM㧘NaCl 1 M㧘EDTA 10 mM
ߢ޽ࠆ㧚EDTA ࠍ౉ࠇߡ޿ࠆߩߪ㧘2 ଔߩ㓁ࠗࠝࡦߦࠃߞߡ

electrodeDNA ห჻߇㕖․⇣⊛ߥๆ⌕ࠍ߅ߎߒ㧘ࡂࠗࡉ࡝࠳ࠗ࠯࡯࡚ࠪࡦߣ඙೎ߢ߈ߥߊߥࠆߩࠍ㒐ߋߚ߼ߢ޽ࠆ㧚࿑ 7 ߇ታ㛎ߩ⚿ᨐߢ޽ࠆ㧚࿑ a)ߢ࠹ࡦࡊ࡟࡯࠻ DNA ߇ሽ

ss-DNA bridge࿷ߔࠆ૏⟎ߦ㧘࿑ b)ߢࡊࡠ࡯ࡉ DNA ߇ሽ࿷ߒߡ߅ࠅ㧘࠹ࡦelectrodeࡊ࡟࡯࠻ DNA ߩߥ޿૏⟎ߦߪࡊࡠ࡯ࡉ DNA ߇޿ߥ޿ߎߣ߆ࠄ㧘ࡊࡠ࡯ࡉ DNA ߇࠹ࡦࡊ࡟࡯࠻ DNA ߦ⚿วߒߡ޿ࠆߣ್

࿑ 5 䊐䊨䊷䊁䉞䊮䉫䊘䊁䊮䉲䊞䊦䈮ਔ┵䈏り㐳࿕ᢿߐࠇࠆ㧚ߒ߆ߒߥ߇ࠄ㧘ߘߩ⚿ว૏⟎ߪ㧘⋧⵬㈩೉ߩ޽ࠆ

ቯ䈘䉏䈢৻ᧄ㎮ DNA, 㔚ᭂ䉩䊞䉾䊒 = 2PP
Fig.5 Stretched single-stranded DNA anchored atboth ends onto the floating-potential electrode;࠹ࡦࡊ࡟࡯࠻ DNA ਛᄩઃㄭߦᔅߕߒ߽㒢ቯߐࠇࠆ߽ߩߢߪ
ߥ߆ߞߚ㧚߹ߚ㧘⚿วߩേᘒߩ⚻ᤨⷰኤߦࠃࠇ߫㧘ࡊࡠ࡯ࡉDNA ߇৻ᣤ࠹ࡦࡊ࡟࡯࠻ DNA ߦធ⸅ߔࠆߣ㧘ߘߩ૏⟎ߢ⚿gap length = 2PP- 4 -24

静電気学会誌

第 29 巻

第 1 号（2005）

ss-DNAAl electrodess-DNAAl electrode

Al electrode
Al electrode(a) Blue excitation (template DNA)(a) Blue excitation (template DNA)Al electrode

Al electrodeprobe DNAAl electrode

Al electrode(b) Green excitation (probe DNA)(b) Green excitation (probe DNA)
࿑ 6⚐☴ਛߢߩ࠹ࡦࡊ࡟࡯࠻ DNA ߣࡊࡠ࡯ࡉDNA ߩࡂࠗࡉ࡝࠳ࠗ࠯࡯࡚ࠪࡦ
Fig.6 Hybridization of the DNA template and the probein pure water.

࿑7ࡃ࠶ࡈࠔ࡯ਛߢߩ࠹ࡦࡊ࡟࡯࠻ DNA ߣࡊࡠ࡯
ࡉ DNA ߩࡂࠗࡉ࡝࠳ࠗ࠯࡯࡚ࠪࡦ
Fig.7 Hybridization of the DNA template and the probe ina buffer.

วߒߡߒ߹޿㧘⸃㔌ߒߚࠅ,⒖േߒߚࠅߔࠆ᭽ሶ 8-9)ߪⷰኤߐ

࿑ 4 b)ߦ߅޿ߡ㧘ߐࠄߦ㔚⇇ࠍ਄᣹ߐߖߡ߽ߎࠇએ਄ߩિ
㐳߇⷗ࠄࠇߥ߆ߞߚ੐ታߪ㧘㔚᳇ജߪႮၮߩࠬ࠲࠶ࠠࡦࠣࠍ

ࠇߥ߆ߞߚ㧚ᒁ߈೸߇ߔ߶ߤᒝߊߥ޿ߎߣࠍ␜ໂߒߡ޿ࠆ߽ߩߣᕁࠊࠇࠆ‫ޕ‬7.

ᓥߞߡ㧘1 ᧄ㎮ DNA ߇ 2 ᧄ㎮ DNA ߩ 2/3 ⒟ᐲߩ㐳ߐߦ߹ߢ⼏⺰એ਄ߩ⚿ᨐࠍ⸃㉼ߔࠆߦ޽ߚߞߡߪ㧘߹ߕ㧘࠹ࡦࡊ࡟࡯࠻િ㐳ߐࠇߚ࿑ 4 b)ߩ⚿ᨐ߆ࠄ㧘߽ߒ 1 ᧄ㎮ DNA ߩਛߢ㎮ౝDNA ߇㧘ࡊࡠ࡯ࡉ DNA ߩ⚿ว߇น⢻ߥࠃ߁ߦ࿕ቯߐࠇߡ޿Ⴎၮኻว߇↢ߓߡ޿ࠆߣߒߡ߽৻ㇱߩߺߢ޽ࠈ߁‫ޕ‬ታ㓙㧘િࠆ߆߇໧㗴ߣߥࠈ߁‫ ߒ߽ޕ‬DNA ߩ⢛㛽߇⋥✢⁁ߦߥࠆ߹ߢ 1

㐳࿕ቯ 1 ᧄ㎮ DNA ߦࠗࡦ࠲࡯ࠞ࡟࡯࠲ࠍᷝടߒߡ߽ᒙ޿Ⱟ

ᧄ㎮ DNA ߇િ㐳ߐࠇࠇ߫㧘2 ᧄ㎮ DNA ߩ⚂ 2 ୚ߩ㐳ߐࠍᜬశߒ߆ⷰኤߐࠇߥ޿ߣ޿߁੐ታߪ㧘ߎߩߎߣߩற⸽ߢ޽ࠆ‫ޕ‬10)ߦ߅޿ߡߪ㧘1 ᧄ㎮ൻߒߚ⥄ὼ߆ߟ㧘߽ߒ㎮ౝႮၮኻࠍᒻᚑߒߡ޿ࠆߣߒߡ߽㧘޽ࠆ޿ߪႮ

㐳16Pm ߩODNA ߇ᵹ૕ജࠍ↪޿ߡ20Pm એ਄ߦિ㐳ߐࠇߚၮ㑆ߩ⋧੕૞↪ߦࠃߞߡㇱಽ⊛ߦಝ㓸ߒߡ޿ࠆߣߒߡ߽㧘ߘߎߣ߇ႎ๔ߐࠇߡ޿ࠆ‫ࠄ߇ߥߒ߆ߒޕ‬㧘ߎߩࠃ߁ߥࠝ࡯ࡃ࡯

ࠇ߇ߚߣ߃߫ᢙච base એ਄ߩ㐳޿㗔ၞߢߥ޿㒢ࠅ㧘ࡊࡠ࡯ࡉࠬ࠻࡟࠶࠴ࡦࠣߪ㧘Ⴎၮߩࠬ࠲࠶ࠠࡦࠣ᭴ㅧࠍ⎕უߒ㧘⋧⵬

DNA ߇┹ว⊛ߦ⚿วߢ߈ࠆ߽ߩߣᕁࠊࠇࠆ‫ޕ‬ߟߪߕߢ޽ࠆ‫ޕ‬ታ㓙㧘ᢥ₂⊛ߥ DNA ߩ⚿วࠍ࿎㔍޽ࠆ޿ߪਇน⢻ߦߒߡߒ߹߁‫ߥߔޕ‬ߎࠇࠄߩߎߣ߆ࠄ㧘ᧄ⎇ⓥߢᓧࠄࠇߚિ㐳࿕ቯ 1 ᧄ㎮ DNAࠊߜ㧘ࡂࠗࡉ࡝࠳ࠗ࠯࡯࡚ࠪࡦߩߚ߼ߦߪ㧘2 ᧄ㎮ DNA ߩ⥄ߪ㧘ࡊࡠ࡯ࡉ DNA ߣߩ⋧੕૞↪ࠍⷰኤߔࠆߚ߼ߩ࠹ࠬ࠻ࡌὼ㐳એ਄ߦ 1 ᧄ㎮ DNA ࠍિ㐳ߔࠆߎߣߪ᦭ኂߢ޽ࠆ‫ޕ‬߹ߚ㧘࠶࠼ߣߒߡචಽߦ೑↪น⢻ߥ߽ߩߢ޽ࠆߣ⠨߃ߡ޿ࠆ‫ޕ‬ߎߩࠃ߁ߥ࠹ࡦࡊ࡟࡯࠻ࠍ↪޿ߡⴕߞߚ࿑ 7 ߩⷰኤ⚿ᨐߪ㧘Ⴎၮߩࠬ࠲࠶ࠠࡦࠣߩS⚿ว߿Ⴎၮ㑆ߩ⇹᳓⋧੕૞↪ߪ㧘Ⴎၮ㑆ߩᒁജߣߥࠆߪߕߥߩߢ㧘ߎࠇࠄߩ⚿ว߇⎕უߐࠇߥ޿ࡊࡠ࡯ࡉࠍ࠹ࡦࡊ࡟࡯࠻਄ߩ⋧⵬㈩೉ߦല₸⦟ߊ⚿วߐߖࠆ⒟ᐲߩㆡᐲߥജࠍട߃ߡિ㐳ߒߚ႐วߦߪ㧘1 ᧄ㎮ DNA ߩિߚ߼ߩ৻ߟߩ␜ໂࠍਈ߃ࠆ߆߽ߒࠇߥ޿‫ߜࠊߥߔޕ‬㧘RNA ࡐ㐳㐳ߐߪ 2 ᧄ㎮ DNA ߩ႐วࠃࠅ߽⧯ᐓ⍴ߊߥࠆߎߣߔࠄ⠨࡝ࡔ࡜࡯࠯ 8)߿೙㒢㉂⚛ 9)ߪ㧘ߘߩ⚿วߔߴ߈㈩೉ࠍត⚝ߔ߃ࠄࠇࠆ‫ޕ‬ࠆߚ߼㧘1) ࡊࡠ࡯ࡉ߇࠹ࡦࡊ࡟࡯࠻਄ߩછᗧߩ૏⟎ߦ߹ߕᒙ- 5 -分子組立の鋳型としての 1 本鎖 DNA の伸長固定（小林・鷲津）ಽሶ⚵┙ߩ㍌ဳߣߒߡߩ 1 ᧄ㎮ DNA ߩિ㐳࿕ቯ㧔ዊᨋࠄ㧕25ߊ㕖․⇣⊛ߦ⚿วߒ㧘2) ߘߎ߆ࠄ࠹ࡦࡊ࡟࡯࠻਄ࠍ 1 ᰴర᜛ᕈࠍઃടߔࠆߎߣߦ߽ᓎ┙ߟߣᕁࠊࠇࠆߩߢ㧘ߎߩ㕙߆ࠄߩᢔ㧔ࠬ࡜ࠗ࠺ࠖࡦࠣ㧕ߔࠆߎߣߦࠃࠅ⋡⊛ߩ㈩೉ߦ೔㆐ߒ㧘⎇ⓥ߽ㅴ߼ߡⴕ߈ߚ޿.

3) ᦨ⚳⊛ߦ․⇣⊛ߥ⚿วࠍᒻᚑߔࠆ㧘ߣ޿ߞߚㆊ⒟ࠍ⚻ࠆߣߐࠇߡ޿ࠆ‫ߩߎޕ‬ㆊ⒟ߪ㧘නߥࠆ 3 ᰴర᜛ᢔߦࠃࠅ⋡⊛ߩ㈩ᧄ⎇ⓥࠍㅢߓߡ㧘᧲੩ᄢቇߩዊⓣ⧷ᑝ⻠Ꮷ࡮ᧁ᧛␭ผඳ჻㧘೉ࠍ஧ὼߦࡅ࠶࠻ߔࠆߎߣࠍᦼᓙߔࠆࠃࠅ㧘ߪࠆ߆ߦല₸⊛(ᩣ)ࠕ࠼ࡃࡦࠬߩ㤥Ỉୃ᳁㧘(ᩣ)࠰࠾࡯ߩ᧻ᧄᴕ਎ሶ᳁ߦߪ㧘ߥߪߕߢ޽ࠆ‫ ࡉ࡯ࡠࡊޕ‬DNA ߇࠹ࡦࡊ࡟࡯࠻ DNA ߦ⚿วߔ⾆㊀ߥᓮ⼏⺰࡮ߏഥജࠍ⾦ࠅ߹ߒߚ.ᧄ⎇ⓥߪ㧘↢⎇࠮ࡦ࠲࡯ࠆߚ߼ߦ߽㧘ߎߩࠃ߁ߥ㕖․⇣⚿วߦࠃࠆਛ㑆૕ࠍ⚻ࠆࡊࡠᣂ੐ᬺഃ಴⎇ⓥ㐿⊒੐ᬺ‫ޟ‬ㆮવሶߩಽሶ࡟ࡌ࡞ᠲ૞ᛛⴚߩ㐿࠮ࠬࠍ೑↪ߢ߈ࠇ߫ࠃࠅല₸⊛ߢ޽ࠆߎߣߪ⸒߁߹ߢ߽ߥ޿‫ޕ‬⊒‫ޠ‬㧘߅ࠃ߮(ᩣ)࠰࠾࡯ߩഥᚑࠍฃߌⴕࠊࠇߚ߽ߩߢߔ.ߎߎߦ

㕖․⇣⚿วᓟߩ 1 ᰴర᜛ᢔ߇㧘RNA ࡐ࡝ࡔ࡜࡯࠯߿೙㒢㉂⚛

⻢ᗧࠍ⴫ߒ߹ߔ.

ߩ႐วߩࠃ߁ߥࠬ࡜ࠗ࠺ࠖࡦࠣߢ޽ࠆ߆㧘ㄭ〒㔌ߢ⚿ว࡮⸃ෳ⠨ᢥ₂1) J. Chen and N.C. Seeman: Nature 350 (1991) 631
2) W. Fritzsche ed.: American Institute of Physics Conference
Proceedings 640 (2003)
3) W. Fritzsche ed.: American Institute of Physics Conference
Proceedings 725 (2004)
4) M. Washizu, Y. Kimura, T. Kobayashi, O. Kurosawa, S.
Matsumoto and T. Mamime: American Institute of Physics
Conference Proceedings 725 (2004) 675) S. B. Smith, Y. Cui and C. Bustamante: Science 271 (1996)7956) M. Washizu and O. Kurosawa: IEEE Trans. IA 26 (1990)
1165
7) T. Yamamoto, O. Kurosawa, H. Kabata, N. Shimamoto and
M. Washizu: IEEE Transaction IA, 36 (2000) 1010
8) H.Kabata, O.Kurosawa, I.Arai, M.Washizu, S.A.Margarson,R.E.Glass and N.Shimamoto: Science 262 (1993) 1561
9) H. Kabata, W. Okada and M. Washizu: Jpn. J. Appl. Phys.
39 (2000) 7164
10) A. M.van Oijen, P. C.Blainey, D. J.Crampton, C. C.
Richardson, T. Ellenberger and X.S. Xie: SCIENCE 301(2003) 1235
11) L. P. Feng, S. H. Park, J. H. Reif and H. Yan: Angew. Chem.
Int. Ed. 42 (2003) 4342

㔌ࠍ➅ࠅ㄰ߔࡎ࠶ࡇࡦࠣߢ޽ࠆ߆㧘Ⴎၮ⋧੕૞↪ߦၮߠߊDNA walk11)ߩࠃ߁ߥ߽ߩߢ޽ࠆ߆ߪ੹ᓟߩ⎇ⓥߦᓙߚߑࠆ

ࠍᓧߥ޿߇㧘޿ߕࠇߦߖࠃ㧘㕖․⇣⚿ว߇ᒙߔ߉ࠇ߫㧘ߔߋߦߪߕࠇߡߒ߹޿㧘᦭ലߥ⒖േ߇ߢ߈ߥ޿ߒ㧘ㅒߦ㧘㕖․⇣⚿ว߇ᒝߔ߉ࠇ߫㧘߿ߪࠅ⒖േ߇ߢ߈ߥߊߥࠆߢ޽ࠈ߁‫ޕ‬ᓥߞߡ㧘ᦨ⚳⊛ߥ․⇣⊛⚿วߩ෼₸ࠍ㜞߼ࠆߚ߼ߦߪ㧘⒖േࠍല₸ࠃߊ↢ߓߐߖࠆࠃ߁ߥᦨㆡߥ㕖․⇣⚿วߩ⸳⸘߇㊀ⷐߢ޽ࠆߣ⠨߃ࠄࠇࠆ‫ޕ‬ߎߩⷞὐ߆ࠄ⷗ߡ㧘࿑ 7 ߢ⋧⵬㈩೉߳ߩ⚿ว߇߶ߣࠎߤ⷗ࠄࠇߥ߆ߞߚߎߣߪ㧘ࡊࡠ࡯ࡉ DNA ߇㐳ߔ߉ߡᄙᢙߩ࡜ࡦ
࠳ࡓߥႮၮኻ߿⇹᳓⚿วߥߤ߇↢ߓߚߚ߼㧘਄⸥ߩ㕖․⇣⚿ว߇ᒝߔ߉ߚߎߣ߇ේ࿃ߢߪߥ޿߆ߣ⠨߃ߡ޿ࠆ‫੹ޕ‬ᓟ㧘⍴޿ࡊࡠ࡯ࡉࠍ↪޿ࠆ࡮᷷ᐲ߿ႮỚᐲߩᦨㆡൻࠍⴕ߁ߥߤߩᣇ╷ߦࠃࠅ㧘ߎߩ㕖․⇣⚿วߩᒝᐲࠍ೙ᓮߒ㧘ല₸ߩࠃ޿ࡂࠗࡉ࡝࠳ࠗ࠯࡯࡚ࠪࡦࠍ⋡ᜰߒߡⴕߊ੍ቯߢ޽ࠆ‫ޕ‬ߥ߅㧘ᧄ⎇ⓥࠍㅢߓߡᓧࠄࠇߚಽሶ⚿วㆊ⒟ߦ㑐ߔࠆ⍮⷗ߪ㧘DNA ࠴࠶ࡊ߿ FISH ߩ㜞ല₸ൻ߿㧘ߘࠇࠄߩᚻᴺߦቯ㊂- 6 -