NIPPON Genetics EUROPE GmbHwww.nippongenetics.eu
www.n-genetics.com

SourceAcinetobacter calcoaceticus

Restriction Enzyme
Acu I
Cat.#FG-AcuIReaction conditions at 37°C

Size
300 unitsConc.
5 units/μl

1X FastGene® Buffer Ⅳ, 40 uM S-adenosylmethionine(SAM),
1X FastGene® FastCut Buffer, 40 uM S-adenosylmethionine(SAM),

FastGene® FastCut Buffer
FastGene® restriction enzyme can cut substrate DNA in 5-15 minwith FastGene® FastCut Buffer.

Store at -20℃
Supplied with: 10X FastGene® Buffer IV(FG-REB4)
10X FastGene® FastCut Buffer (FG-REBHF)
S-adenosylmethionine
6X DNA Loading BufferSterile water

1X FastGene® Buffer IV

Recognition site

Unit definition` ’ ∙ ∙ ∙ C T G A $ *  1  ∙ ∙ ∙ 3 ’
3 ’ ∙ ∙ ∙ G A C T 7 &  1  ∙ ∙ ∙  ’

20 mM Tris-acetate (pH 7.9 at 25℃)
50 mM potassium acetate
10 mM magnesium acetate
100 μg/ml BSA

One unit is defined as the amount of enzyme required to digest
1 µg of Lambda DNA in 1 hour at 37°C in a total reaction
volume of 50 µl.

Quality control
For Research Use Only. Not for use in diagnostic procedures.

NIPPON Genetics EUROPE GmbH

Dilution buffer- Unit definition assay- Overdigestion assay- Endonuclease assay- Extreme pure assay

Standard reaction condition® Diluent B
www.nippongenetics.eu
FastGene
www.n-genetics.com- Normal protocol
Component

Heat Inactivation

Final Conc.

VolumeSubstrate DNA

1 μg

X μl

10X FastGene® Buffer IV

1X

5 μl

S-adenosylmethionine

40 μM

1 μl

Acu I can be inactivated at 65℃ for 20 min.

SourceRestriction
EnzymeMethylation
sensitivity

Acinetobacter calcoaceticus
Acu IReactionconditions at 37°C

Acu I

dam methylation: Not sensitive
dcm methylation: Not sensitiveCpG methylation: Not sensitive
Cat.#Size
FG-AcuIactivity in FastGene
300 units® Buffers
RelativeConc.
5 units/μl

FastGene® Buffer I:
50%
Store at -20℃
FastGene® Buffer II:
50%75%
FastGene® Buffer III:® Buffer IV:100%
FastGene® Buffer IV
Supplied
with: 10X FastGene(FG-REB4)®
® FastCut Buffer (FG-REBHF)
FastGene FastCut Buffer:
100%10X FastGene
S-adenosylmethionine
6X DNA Loading Buffer
NoteSterile water
Acu I requires S-adenosylmethionine (SAM) for optimal activity.
SAMRecognition(in 0.005 M site
sulfuric acid and 10% Ethanol) stored at -20℃
is stable for at least 6 months. Reaction condition with excess
enzyme, excess glycerol (>5%) or longterm incubation may
 ’ ∙ ∙ ∙ C T G A $ *  1  ∙ ∙ ∙ 3 ’
result in star activity.

`3 ’ ∙ ∙ ∙ G A C T 7 &  1  ∙ ∙ ∙  ’

For Research Use Only. Not for use in diagnostic procedures.

5 unit1 μl® Buffer Ⅳ, 40 uM S-adenosylmethionine(SAM),
1X Sterile
FastGenewaterup to 50 μl
1X FastGene® FastCut Buffer, 40 uM S-adenosylmethionine(SAM),→ Incubate at 37℃ for 1 hr
- Fast protocol

FastGene® FastCut Buffer

Component
FinalConc. DNAVolume
FastGene® restriction
enzyme can cutsubstratein 5-15 min® FastCut Buffer.
Substrate
1 μg
X μl
with
FastGeneDNA
10X FastGene® FastCut Buffer

1XS-adenosylmethionine
FastGene® Buffer IV

20 mM Tris-acetate (pH 7.9 at 25℃)
Acu I
50 mM potassium acetate
water10 SterilemM magnesiumacetate
100 →μg/ml
BSA at 37℃ for 15 min
Incubate1X5 μl

40 μM

1 μl

5 unit1 μl
up to 50 μl

Unit※definition
We recommend 5-10 units of enzyme per µg DNA and 10One20
unit
is definedas theDNA
amountunits
for genomic
in a of1 henzyme
digest. required to digest
1 µg of Lambda DNA in 1 hour at 37°C in a total reaction
volume of 50 µl.

Quality control- Unit definition assay- Overdigestion assay- Endonuclease assay- Extreme pure assay

Standard reaction condition