基調講演 2

Establishment of patient derived xenograft(PDX) and PDX derived cell lines from liverfluke associated cholangiocarcinoma tissue.
Patient-derived xenograft (PDX) models are created by engraftment of patient’s tumor
tissues into immunocompetent mice. Since PDX model keep the characteristics of primarypatient’s tumor such as gene expression profiles and drug sensitivity, it now becomes mostreliable in vivo human cancer model. The engraftment rate are increased with the introductionof NOD/Scid based immunocompromised mice, especially, NK cell defective NOD strains: NOD/nu

Scid/IL2Rγnull(NOG/NSG) mice and NOD/Scid/Jak3(NOJ) mice. BALB/c Rag-2/Jak3 double

deficient (BALB/c R/J) mice were also established and have been used as preferable recipient

Seiji Okada
Division of Hematopoiesis
Joint Research Center for Human Retrovirus Infection& Graduate School of Medical Sciences, Kumamoto University[Awards]
1992

Japan Association for Development of Community Medicine, Research Award

1993

Japanese Society of Hematology, Young Investigator’s Award

1999

Mochida Memorial Foundation for Medical and Pharmaceutical Research, Research Award

2000

Chiba University Inohana Alumni Association, Academic Award2010Japan Leukemia Research Fund, Research Award

2017

Kumamoto University Education Activity Award

2018

The Honorary Doctorate Degree of Medicine, Khon Kaen University, Thailandfor PDX.
Liver-fluke associated cholangiocarcinoma (CCA) is a major health problem in Southeast
Asia, particularly countries along the Mae Khong River. The critical obstacles of CCA diagnosis
and treatment are the high heterogeneity of disease and considerable resistance to treatments.
Recent multi-omics studies revealed the promising targets for CCA treatment; however, limited
models for drug discovery is available. We transplanted 16 frozen CCA tissues into BALB/c R/J mice subcutaneously, and 12 CCAs (8 intrahepatic and 4 extrahepatic subtypes) were[References]
1. Vaeteewoottacharn K, Pairojkul C, Kariya R, Muisuk K, Imtawil K, Chamgramol Y, Bhudhisawasdi
V, Khuntikeo N, Pugkhem A, Saeseow OT, Silsirivanit A, Wongkham C, Wongkham S, *Okada S.
Establishment of Highly Transplantable Cholangiocarcinoma Cell Lines from a Patient-Derived
Xenograft Mouse Model. Cells . 2019. 23;8(5). pii: E496
2. Sittithumcharee G, Suppramote O, Vaeteewoottacharn K, Sirisuksakun C, Jamnongsong S, LapanuwatP, Suntiparpluacha M, Matha A, Chusorn P, Buraphat P, Kakanaporn C, Changkeaw K, Silsirivanit

successfully grown and sub passaged in BALB/c R/J mice. Five CCA cell lines were establishedA, Korphaisarn K, Limsrichamrern S, Tripatara P, Pairojkul C, Wongkham S, Sampattavanich S,

from 12 PDX tissues. The effects of CDK4/6 inhibitor was evaluated and confirmed in PDX

Okada S, *Jirawatnotai S. Dependency of Cholangiocarcinoma on Cyclin D-Dependent Kinase Activity.

model. Thus, PDX and PDX derived cell lines would be a useful platform tor precision cancermedicine.

Hepatology 2019 in press
3. *Okada S, Kariya R, and Vaeteewoottacharn K. Establishment of Patient-derived tumor xenograft (PDX)
model and application for precision cancer medicine. Chem Pharm Bull (Tokyo) 66:225, 2018
4. 岡田誠治．患者腫瘍移植マウスモデル（Patient-Derived Tumor Xenograft：PDX）とその活用－個別化がん治療（Precision Cancer Medicine）に向けて－ Cytometry Research 27:51, 2017

Seiji Okada, MD, PhD
Professor, Joint Research Center for Human Retrovirus Infection& Graduate School of Medical Sciences, Kumamoto University
1985
1985
1992
1992
1996
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2006
2019

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Degree received M.D. Jichi Medical School, School of Medicine
Medical Officer, Ibaraki Prefecture
Degree received Ph.D., Jichi Medical School
Post Doctoral Fellow, Research Institute of Molecular Pathology, Vienna
Assistant Professor, Center for Biomedical Science, Chiba Univ. School of
Medicine,Associate Professor, Chiba Univ. Graduate School of Medicine,Professor, Center for AIDS Research & Graduate School of Medical Sciences,
Kumamoto Univ.
Director, Radioisotope Center, Kumamoto Univ.
Professor, Joint Research Center for Human Retrovirus Infection& Graduate School of Medical Sciences, Kumamoto Univ.

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