NIPPON Genetics EUROPE GmbHwww.nippongenetics.eu
www.n-genetics.com

Source: Streptomyces caespitosus

Restriction Enzyme
Sca I
Cat.#FG-ScaISize
1,000 units

Reaction conditions
1X FastGene® Buffer III, 37℃
1X FastGene® FastCut Buffer, 37℃
Conc.
4 units/μl

FastGene® restriction enzyme can cut substrate DNA in 5-15
min with FastGene® FastCut Buffer.

1X FastGene® Buffer III

Store at -20℃
Supplied with:

FastGene® FastCut Buffer

10X FastGene® Buffer III(FG-REB3)
10X FastGene® FastCut Buffer (FG-REBHF)
6X DNA Loading BufferSterile water

50 mM Tris-HCl (pH 7.9 at 25℃)
100 mM NaCl
10 mM MgCl2
100 μg/ml BSA

Unit definition
One unit is defined as the amount of enzyme required forcomplete digestion of 1 μg bacteriophage λ at 37℃ for 1 hr in
50 μl reaction mixtures.

Recognition site

Quality control- Unit definition assay- Overdigestion assay- Endonuclease assay- Extreme pure assay

Dilution buffer
FastGene® Diluent A

For Research Use Only. Not for use in diagnostic procedures.

Standard reaction condition

Heat Inactivation

Genetics EUROPE GmbHSca I can beNIPPON
inactivated
at 80℃ for 20 min.

- Normal protocol
Component

www.nippongenetics.eu
Methylation
sensitivity
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Source:Streptomyces
10X FastGene
Buffercaespitosus
III

CpG methylation: Not sensitive

Restriction Enzyme
A minimum
Sca I amount of enzyme required to digest 1-μg substrate
Prolonged incubation
DNA for 16 hr; 0.25 U.

Cat.# activity in FastGene
Size ® Buffers
Relative

Final Conc.

Substrate DNA

dam methylation: Not sensitive
dcm methylation: Not sensitiveConc.
4 units/μl

FG-ScaI
1,000Notunits® Buffer I:FastGene
recommended
Not recommended
FastGene® Buffer II:
Store ®atBuffer-20℃III:
FastGene100%
Not recommended
FastGene® Buffer IV:
100%
FastGene® FastCut Buffer:® Buffer III® Buffer
Supplied
with:I, 10X
FastGene
FastGene
II andIV are notrecommended(FG-REB3)
due to star
10X FastGene® FastCut Buffer (FG-REBHF)
activity
6X DNA Loading Buffer
Sterile water
Note

It is not affected by dam, dcm, and mammalian CpG methylation.
Recognition site
Low-purity DNA is not cleaved efficiently. Reaction condition oflow salt, excess enzyme, excess glycerol (>5%) or high pH (>8.0)
may result in star activity. To avoid star activity, do not use Sca Iin FastGene® buffer I, II, or IV.

For Research Use Only. Not for use in diagnostic procedures.

Sca IReaction
conditions

Volume

1 μg

X μl

1X

5 μl

4 unit® Buffer III, 37℃
1X Sterile
FastGenewater
1X FastGene® FastCut Buffer, 37℃→ Incubate at 37℃ for 1 hr

1 μlup to 50 μl

FastGene® FastCut Buffer- Fast protocol
FastGene® restriction enzyme can cut substrate DNA in 5-15® FastCut Buffer. Final Conc.
Component
Volumemin with FastGene
Substrate DNA

1X FastGene® Buffer III

50 10XmMFastGene
Tris-HCl ®(pH
7.9 at Buffer25℃)
FastCut100 mM NaCl
ScaI
10 mM MgCl2100Sterile
μg/mlwater
BSA

1 μg

X μl

1X

5 μl

4 unit1 μlup to 50 μl

Incubate at 37℃ for 15 min
Unit→definition
One unit is defined as the amount of enzyme required for※ We recommend 5-10 units of enzyme per µg DNA and 10complete digestion of 1 μg bacteriophage λ at 37℃ for 1 hr in
20 units for genomic DNA in a 1 h digest
50 μl reaction mixtures.

Quality control

Dilution buffer- Unit definition assay- Overdigestion assay- Endonuclease assay- Extreme pure assay

FastGene® Diluent A