Sartorius­Pipetting and
Dispensing
Products

Table of Contents

03

Table of Contents
The Sartorius product portfolio, technical data and specifications are subject tochange, due to continuous product development.
Please visit the website for an up-to-date overview of Sartorius products:
www.sartorius.com

About Sartorius Pipetting and Dispensing
Introduction
Quality46

How to Choose a Pipette
Electronic or Mechanical Pipette8Electronic Pipettes
Picus® and Picus® Nxt Electronic Pipettes

12

Mechanical Pipettes
Tacta® Mechanical Pipettes
Mline® Mechanical Pipettes
Proline® Plus Mechanical Pipettes
Proline® Mechanical Pipettes

20
26
32
39

Stands and Accessories
Pipette Stands
Elbow Pad
Safe-Cone Filters
Reagent Vessel
Adjustment Tool

46
47
48
50
50

Pipette Tips
Pipette Tips
Optifit Tips
Safetyspace™ Filter Tips
Low Retention Tips
Packaging Options

54
56
57
58
60

Maxi-volume Liquid Handling
Midi Plus Pipetting Controller
Prospenser Plus & Prospenser
Biotrate Digital Burette

68
70
72

Pipetting Academy
Pipetting Academy
Pipetting Recommendations

78
80

Calibration and Maintenance
Pipette Calibration & Maintenance Services
Pipette Decontamination Procedure
Autoclaving Instructions
Sales and Service Contacts

84
85
86
88

04

Sartorius Pipetting and Dispensing

Introduction

Introduction
Three key factors – ergonomics, design and reliability – form the cornerstone of
our product development. These and other factors have been combined to
produce a perfectly balanced mechanical pipette, the Tacta®, the newest family
member, following the Picus® Nxt, which is an excellent example of an ­electronic
pipette that has all these aspects combined. Tacta® and Picus® are among thelightest pipettes on the market, reducing the risk of Work Related Upper Limb
Disorder (WRULD). Their high reliability, and ease of use, make them valued
instruments for professionals, who strive for high quality results.
Their functional and well-rounded design has been recognised with design
awards. All Sartorius pipettes are designed and manufactured in Finland, where
our R&D team is constantly seeking solutions to further improve liquid handling
instruments to make lab work easier.
“Designing products that people work with on a daily basis is always challenging.
Many users are interviewed and multiple aspects need to be taken into account,to combine excellent ergonomics and easy usability with today’s technology andfeatures. To solve this puzzle and come up with a great product is an exciting, butsometimes tough, journey. However, it is always rewarding in the end.”– Ville Hintikka, Chief Designer at Sartorius
Ergonomics
When designing a pipette, we always consider the shape and function of thehuman hand. As we understand the risks of repetitive pipetting, we emphasize
ergonomic design in every product we make. Simply put, this means productsthat you can use in a comfortable posture with minimum muscle power. Our
pipettes and dispensers are designed for both right- and left-handed users.
Their operating buttons are located sufficiently close together, within ergonomic
reach of the thumb.
DesignWe provide products with a timeless and light, yet practical, design, suitable
for laboratory settings and pleasing to the eye of the user. The Tacta® and the­Picus®, won the Red Dot design award, in 2016 and 2012, respectively. The Picus®
was distinguished with the Fennia Prize Honorary Mention in 2012. The Tacta®
and the Picus®, won the German Design Award in 2017 and 2014, respectively.

Sartorius Pipetting and Dispensing

Reliability
For us, reliability has many aspects, the most important­being accuracy and precision of results and secured purity.
The core of a pipette lies in its accuracy and precision.
For this reason, we have used the newest technologies­together with in-house innovations, to achieve even morereliable pipetting results. Our electronic brake, piston­control system and plate tracker for electronic pipettes are
our latest innovations. They increase accuracy, precisionand reliability of the device. Another important factor inachieving reliable results is the optimal tip fit, which we canguarantee by designing and producing the tips ourselves,
so that they perfectly match our pipettes.
To reduce the risk of contaminating the internal­components of our pipettes, we offer special Safe-Cone­Filters to be used in our pipettes, as we understand that
purity is a key concern in many laboratories. We strive to
produce as many autoclavable products as possible, both
pipettes and tips. Our pipette tips are manufactured in
ISO Class 8 Cleanroom conditions. We test every certified
tip lot for DNase, RNase and endotoxins at an external­laboratory. We also offer an innovative Safetyspace™ Filter
Tip range for safer and contamination-free pipetting.

Introduction

05

06

Sartorius Pipetting and Dispensing

Quality

Quality
Sartorius’ products are developed and manufactured according to the­requirements of the ISO 9001, ISO 13485 and ISO 14001 quality and­environmental standards. Tip production also follows the ISO 14644-1 standard,in order to fulfil ISO class 8 cleanroom conditions.
We continuously develop our products and processes in order to meet, andoften exceed, the demands of regulatory authorities, environmental bodies,and most importantly, our customers.

Sartorius’ products are developed and manufactured according to the requirements of theISO 9001, ISO 13485 and ISO 14001 quality and environmental standards. Tip production
also abides by the ISO 14644-1 standard, in order to fulfil ISO Class 8 Cleanroom conditions.
ISO 13485 is a specific standard for medical device quality systems, and supplementing themore generic ISO 9001 standard, which applies to many industries.

The Finnish national accreditation body operates independently as part of the Measurement
Technology Centre (MIKES). Accredited pipette calibration laboratories in Finland, Germany,
France, UK, China and Japan calibrate pipettes according to precise technical requirements.
Our calibration laboratories in Finland, Germany, France, UK, China and Japan have been
­granted this status by their national accreditation bodies.

During production and service, pipette performance testing is carried out according toISO 8655 specifications. Sartorius accredited pipette calibration follows the ISO 17025 standard.
Our pipettes are supplied with individual quality control certificates.

Sartorius offers a 2-year warranty for all mechanical and electronic pipettes. The low lifetime
cost and environmental friendliness of our products, which have long warranty periods, give ahigh return on investment.

----

We follow these manufacturing quality standards:
ISO 9001
ISO 13485
ISO 14001
ISO 17025
ISO 8655

Sartorius Pipetting and Dispensing

Quality

The ergonomic design label indicates products, which Sartorius has designed specifically to
reduce the risk of work-related hand, arm and shoulder disorders, such as Work Related Upper
Limb Disorder (WRULD).

The Optiload tip loading mechanism developed by Sartorius in Tacta®, Mline®, Proline® Plus,
Picus® and Picus® Nxt pipettes allows tips to be loaded with constant force. This secures­optimal tip sealing and minimum tip ejection force.

The Optilock®, volume locking system in Tacta® gives the choice of locking and unlocking thevolume the traditional way, using both hands, or specially developed convenient method, using
one hand.

The Optiject®, is a unique mechanism in Tacta®, where the soft, levered tip ejection feature lets
the tip detach in a smooth, controlled way with little force. Additionally the feature allows forhands-free ejection of the Safe-Cone filter for a truly safe disposal of contaminated filters.

Every lot of Sartorius Single Tray, Refill Pack and FlexiBulk® tips are certified to be free of DNase,
RNase, human DNA and endotoxins, for the protection of samples from contamination. These
lot specific and other certificates can be downloaded from www.sartorius.com.
Sartorius’ tip production is ISO 8 cleanroom classified, which ensures a contamination-freemanufacturing environment, and products.

Most Sartorius pipetting and dispensing products are autoclavable. Please see details in thefollowing product specific chapters.

07

08

How to Choose a Pipette

Electronic or Mechanical Pipette

How to Choose a Pipette
Electronic or Mechanical Pipette
Are you looking for a pipette for sterile work, or one you could easily calibrateyourself? Or do you seek a really light and ergonomic solution? Perhaps youneed a pipette with a certain pipetting mode to speed up your work? By consulting the tables below, you can choose the instrument that best suits your needs.

Electronic or Mechanical Pipette
Features
Highest Ergonomics
Fastest pipettingUser-independent results
Multiple pipetting modes
Fully autoclavable
Adjustment by user
1 Excluding Proline®

---

Electronic Pipettes

Mechanical Pipettes--

1

Mechanical Pipettes
Tacta®

75 g-

Proline® Plus

Weight1--

Mline®

77 g

82 g

-

84 g

Length1

225 mm

240 mm

239 mm

224 mmVolume range, single-channels

0.1 µL – 10 mL

0.1 µL – 10 mL

0.1 µL – 10 mL

0.1 µL – 5 mL

Volume range, multi-channels

0.5 – 300 µL

0.5 – 300 µL

0.5 – 300 µL

0.5 – 300 µL

12 N

13 N

15 N

20 N

all models

all models

multi-channels only

Features
Most Ergonomic
Ergonomic Finger Hook

Fixed-volume models
Pipetting force1
Optiject soft tip ejection
Light tip ejection
Optiload spring-loaded tip cones
User adjustment
Optilock on | off volume lockVolume locking
Big, and easy to read display
Safe-cone Filters (models > 10 µL)
Filter ejector
Color coding on pipette
ID tags
Fully autoclavable
Multipacks
Pipette holder with pipette
Warranty for 2 years
1 1,000 µL 1-channel models-----------------

click stops

Proline®

--click stops-

How to Choose a Pipette

Electronic or Mechanical Pipette

Electronic Pipettes
Features--Picus® Nxt

Picus®

Weight1

100 g

100 g

Length1

210 mm

210 mmVolume range, single-channels

0.2 µL – 10 mL

0.2 µL – 10 mL

Volume range, multi-channels

0.2 µL – 1.2 mL

0.2 µL – 1.2 mL

Language options255

Pipetting modes

98

Advanced functions

7

6

Protocols – memory places

3-

Memory places(for storing programs)

10

10

Most ergonomic

Repeated blow-out(advanced function)
Microwell plate tracker

Reminders for calibration & service
Information on service & calibrationintervals
Password protection
Certificate of accredited
3-point calibration
Electronic tip ejection
Calibration adjustment by user
Calibration adjustment in
1, 2 or 3 points
Hot key for stored programs
Use of pipette while charging
Fully charged in 1 hour
Safe-Cone Filters
Autoclavable lower parts3
Optiload in multi-channels
Color coding on pipette
Warranty for 2 years
1 300 µL 1-channel models
2 English, French, German, Chinese & Russian
3 Excluding 1,200 µL multi-channel pipettes-------------

09

11

Electronic Pipettes
Table of Contents

Electronic Pipettes
Picus® & Picus® Nxt

12

12

Electronic Pipettes

Picus® and Picus® Nxt

Picus® and Picus® Nxt Electronic Pipettes
The Most Sophisticated and Ergonomic Pipettes Ever!
The Picus® family pipettes, ­Picus®
and Picus® Nxt, with Sartorius ­pipettetips are the new standard for lab
professionals. The Picus® ­family­pipettes are kind to your hand with
unbeatable ­ergonomic design that
ensures reliable and repeatable
experiment results. Picus® is the righttool for ­everybody from intern to lab
expert, being as easy to start with asam­ echanical pipette and offering
options for even the most advanced
user. Picus® saves you time in the labwith pipetting modes and customizable programs for every need. The
pipette is easy to get comfortable withand quick to use. Just pick it up, switch
it on and pipet!
Picus® Nxt takes on where Picus® left
with added features like user customizable protocols, password protection,and calibration reminders. ­Especiallywhen conformity to the strictest
regulations is needed by selecting
the ­advanced Picus® Nxt you cantake your compliance to a new levelwith added certification and safety
features. Both Picus® and Picus® Nxt,
are available in single-channel models, covering a volume range of 0.2 to
10,000 µL and in multichannel models
from 0.2 µL to 1,200 µL. Choose thetools that any professional deserves.

Superior Ergonomics
The uniquely lightweight and streamlined design of the Picus® and Picus®
Nxt pipette ensures an effortless
pipetting experience. The pipette rests
lightly in the user’s hand due to the
comfortable handle and finger hook,and minimal gripping force is needed.
The conveniently located soft-touch
operating buttons, and uniqueelectronic tip ejection, help minimize
muscle strain, further enhancing theergonomics of the pipette.
Reliable Results
The electronic brake and piston control system guarantee accurate andprecise pipetting results, independentof the user. Using the unique plate
tracker increases reliability in microwellplate dispensing, by guiding the user
to pipet into the correct microplate
wells.

Electronic Pipettes

Picus® and Picus® Nxt

Calibration Adjustment
Gives the user the option to adjust thecalibration according to conditions
and fluids that are to be pipetted.
Fast Execution of Pipetting Tasks
The unique adjustment wheel of
Picus® pipettes offers extremely fast
volume setting and menu navigation.
The user can perform pipetting tasks
quickly and easily with the extensive
range of pipetting modes, from diluting and titrating to serial dispensing.

Picus® and Picus® Nxt are lightweight pipettes, with a comfortable handle and finger hook,requiring minimal gripping force.

The pipetting functions are quick tolearn, using the intuitive user ­interface,
available in a choice of language
options: English, French, German,
Russian and Chinese.
Picus® Nxt Enhancements
Picus® Nxt has the same qualities as
Picus®, and has added features forincreased efficiency and safety, whichmake it suitable for strictly regulated
laboratories.

Adjustment wheel for quick­single-handed operation andspeed control.

Custom Protocols in Picus® Nxt
Protocols are saved to memory in
Picus® Nxt, which speed up ­routine
pipetting sequences. Create 1 – 3­different protocols; multiphase­workflows of up to 10 steps.
Ensure repeatable results. Reduced
number of presses during pipetting.

13

Picus® Nxt for Highly Regulated
Laboratories
The following features are only
­available with Picus® Nxt pipettes,
which are especially intended toconform to the strictest laboratory
regulations andrequirements:-

	Certificate of accredited 3-pointcalibration (per ISO 17025 andISO 8655)
	Reminders alert you when ­regular
maintenance and calibration is­required	Advanced password protection
for settings and pipetting protocol
memory	The pipette can be locked, to­prevent its use, e.g. in case of­contamination
	Custom protocols speed up­routine pipetting sequences and­standardise regulated workflows	Repeated blow-out function helps
dispense every last droplet, ensuring
complete sample recovery

14

Electronic Pipettes

Picus® and Picus® Nxt

Features and Benefits----

Ergonomic Design for Reduced Riskof Strain Injury
	Extremely compact and light(only 100 g) design maximizes
user ­comfort
	Conveniently located, soft-touch
operating button and comfortableelectronic tip ejection help minimize
muscle strain and reduce the risk of
WRULD	Comfortable handle design andfinger hook allow the pipette to rest
effortlessly in hand

Microwell plate tracker in use

Calibration Adjustment in use
as indicated by ADJ1

Innovative Technology for
Reliable Results	Fully electronic functionality provides outstanding accuracy andrepeatability of results	Electronic brake stops piston movement rapidly and precisely, ensuringhigh precision, especially in serial
dispensing	Optical sensor controls and monitors
piston movement in real time,ensuring unbeatable accuracy andreliability	Picus® Nxt only: Repeated blow-out
function helps dispense the very last
droplets of liquid, ensuring complete
recoveryIntuitive User-Interface for Easeof Use	Plug & Play – learn to use in a minute	Natural to use buttons
	Easy menu navigation and uniqueadjustment wheel make for fast
­setting of volumes and modes	Hotkeys for quick access to yourpreset programs	Enables ergonomic single-handed
operation

Comprehensive Range of Pipetting
Modes for Speed of Use
	The main modes combined with theadvanced functions enable fast andhandy execution of various pipettingtasks	Multi-dispensing mode replaces theneed for a stepper---

Microwell Plate Tracker for Improved
Efficiency and Reliability
	Unique built-in tracker for 96 and384 well plates guides the user topipette into the correct wells	Improves work efficiency and­reliability of results

Optiload in Multi-channel Models for
Perfect Tip Sealing	Allows tip loading with an equal­constant force onto every channel
	Enables perfect tip sealing onto
every individual tip cone
Safe-Cone Filters to Prevent­Contamination
	Are available for all models > 10 µL
Autoclavable Lower Parts for Easy
Sterilisation
	For reduced risk of contamination(excl. 1,200 µL models)Calibration Adjustment
	Define and save 3 calibration
settings in addition to the factory
setting	The adjustment can be made in
1, 2 or 3 points by the user
Fast Charge that Lasts Several Hours
	Battery is charged in 1 hour	Continue pipetting while charging
with micro USB cable	Most convenient way is to store yourpipette in a charging standElectronic Pipettes

Picus® and Picus® Nxt

15

Pipetting Modes
Pipetting Modes

Advanced Functions*
Plate
Tracker

PipettingReverse Pipetting
Multi-dispensing
Manual Pipetting
Diluting
Sequential Dispensing
Multi-aspiration
Titration
Protocols** | ***--Mixing-Counter---    * Advanced functions are used in conjunction with the pipetting mode  ** Only available in Picus® Nxt models*** Availability of the advanced functions varies according to the­pipetting mode selected for the protocol

Excess Volume AutoAdjustment
dispensing---

Repeated
Blowout**--

Fast­Dispensing

--

16

Electronic Pipettes

Picus® and Picus® Nxt

Ordering Information
Picus® Nxt

Picus®

Channels

VolumeRange

Increment Test
Volume

(µL)

(µL)

(µL)

Mode P | D

Systematic Error N
Limit ±

Random Error N
Limit

(%)

(µL)

(%)

(µL)

LH-745021

7350211

0.2 – 10

0.01

10
5
1
0.2
1

P
P
P
P
D

1.0
1.2
3.0
17.5
6.0

0.100
0.060
0.030
0.035
0.060

0.4
0.7
2.0
10
7.0

0.040
0.035
0.020
0.020
0.070

LH-745041

7350411

5 – 120

0.10

120
60
12
5
12

P
P
P
P
D

0.5
0.7
2.0
5.5
4.0

0.60
0.42
0.24
0.275
0.48

0.15
0.2
1.0
2.5
4.0

0.18
0.12
0.12
0.125
0.48

LH-745061

735061

1

10 – 300

0.20

300
150
30
10
30

P
P
P
P
D

0.5
0.6
1.5
5.0
3.0

1.50
0.90
0.45
0.50
0.90

0.15
0.2
0.8
2.4
3.0

0.45
0.30
0.24
0.24
0.90

LH-745081

735081

1

50 – 1,000

1.00

1,000
500
100
50
100

P
P
P
P
D

0.45
0.6
2.0
4.0
2.5

4.5
3.0
2.0
2.0
2.5

0.15
0.2
0.5
1.0
2.0

1.5
1.0
0.5
0.5
2.0

LH-745101

735101

1

100 – 5,000

5.00

5,000
2,500
500
100
500

P
P
P
P
D

0.5
0.7
1.6
8.0
2.4

25
17.5
8
8
12

0.15
0.2
0.4
2.0
2.4

7.5
5
2
2
12

LH-745111

735111

1

500 – 10,000 10.00

10,000
5,000
1,000
500
1,000

P
P
P
P
D

0.6
0.9
3.0
7.0
4.0

60
45
30
35
40

0.2
0.3
0.6
1.2
2.4

20
15
6
6
24

LH-745321
LH-745421

7353217354218
12

0.2 – 10

0.01

10
5
1
0.2
1

P
P
P
P
D

1.2
1.5
4.0
25.0
12.0

0.120
0.075
0.040
0.050
0.120

0.5
0.8
3.0
15.0
15.0

0.050
0.040
0.030
0.030
0.150

LH-745341
LH-745441

7353417354418
12

5 – 120

0.10

120
60
12
5
12

P
P
P
P
D

0.6
0.8
2.5
6.0
4.5

0.72
0.48
0.30
0.30
0.54

0.3
0.4
1.67
4.0
8.0

0.36
0.24
0.20
0.20
0.96

LH-745361
LH-745461

7353617354618
12

10 – 300

0.20

300
150
30
10
30

P
P
P
P
D

0.6
0.8
2.33
8.0
3.33

1.80
1.20
0.70
0.80
1.00

0.2
0.3
1.0
3.0
6.0

0.60
0.45
0.30
0.30
1.80

LH-745391
LH-745491

7353917354918
1250 – 1,200

1.00

1,200
600
120
50
120

P
P
P
P
D

0.6
1.0
2.5
8.0
3.33

7.2
6.0
3.0
4.0
4.0

0.2
0.3
1.0
2.4
3.33

2.4
1.8
1.2
1.2
4.0

	Note: The listed systematic and random error values can be achieved only under strictly controlled conditions during type test per ISO 8655. The best compatibility is achieved when combining
Sartorius pipettes and Sartorius tips. The systematic error and random error results, in tests, have been achieved using Sartorius Optifit tips at factory default speed settings. Due to the continuous
product development by Sartorius, the systematic and random error values are subject to change without prior notice.
P
P = Pipetting ModeD
D = Multi-dispensing mode. The listed systematic and random error values are of 10 measurements at 10% of the nominal volume.
All pipettes are supplied with a universal charger (EU, UK, US | JPN, KOR, AUS and CHN plugs)
NElectronic Pipettes

Picus® and Picus® Nxt

17

Tip Selection Guide
Optifit Tip LRTColor-Code

Safetyspace™ TipLRT
VolumeVolumeStandard

Plus

0.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

2 – 120 µL

7210087210185 – 350 µL

5 – 300 µL

721007721017

10 – 1,000 µL

50 – 1,000 µL

721006721016100 – 5,000 µL

100 – 5,000 µL

721005721015–7210057210150.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

2 – 120 µL

7210087210185 – 350 µL

5 – 300 µL

72100772101750 – 1,200 µL

50 – 1,200 µL

721006721016

500 – 10,000 µL

Color-Code–	Note: Low Retention Tips are available in volumes up to 1,200 µL.

LRT

Safe-Cone Filters

19

Mechanical Pipettes
Table of Contents

Mechanical Pipettes
Tacta®
Mline®
Proline® Plus
Proline®

20
26
32
39

20

Mechanical Pipettes Tacta®

Tacta® Mechanical Pipettes
Perfectly Balanced

Have you ever considered the overall
user experience in pipetting?
We have.

Tacta’s® Optilock feature helps
to prevent accidental volume­changes during pipetting.

Easy adjustment ensures accurate
results with a variety of liquids. The
handy adjustment key is includedin the package.

Unique to Tacta®, is its Safe-Cone
Filter ejector, which enables theremoval of used filters without
tweezers or human contact withcontaminated filters.

Sartorius proudly presents Tacta®,
the premium mechanical pipette withsuperb comfort and reliability. Tacta®
makes pipetting effortless and safe,
while producing accurate and reliable
results time after time.
At Sartorius, we listened to our customers and combined their views
with our extensive experience, andstate-of-the-art R&D, to create the­exceptionally balanced design ofTacta®. Tacta® is designed to feel greatin your hand, and it is easy and comfortable to use. All materials used for
Tacta® have been carefully selected,
and each component is designed tomeet the highest standards.
Superior Ergonomics
Tacta® rests lightly in your hand thanks
to its ergonomically designed handle
and finger hook. Tacta® is effortless touse and exceptionally comfortable tohold. Tacta® eases your workload andprotects you from strain, even when
you pipette for extended periods ofyour working day. The unique handle
and finger hook design lets the pipette
rest lightly in your hand, with no needto grip the handle tightly. Tacta® iseffortless to use and exceptionally
comfortable to hold. Tacta® eases yourworkload and protects you from strain,even when you pipette for ­extended
periods of your working day.
The unique handle and finger hookdesign lets the pipette rest lightly inyour hand, with no need to grip thehandle tightly.

Low Pipetting, Tip Loading
and E­ jection Forces
The forces needed for a pipetting
cycle, from tip attachment to pipetting
and finally tip ejection, are exceptionally low with Tacta®, protecting
you from Work Related Upper Limb
­Disorder (WRULD). The Optiloadfeature, with spring-loaded tip cones
in both the single and multi­channel
models, secures tip loading withperfect sealing and minimal force.
­Consequently, Optiload also ensures
light tip ejection to protect yourhand. The light tip ejection is further­enhanced by Sartorius Optiject, thesoft, levered tip ejection featurethat lets the tip detach in a smooth,­controlled way with little force.
Large, Clear Display
The large, easy-to-read display
helps you to see all four digits of theset ­volume from a distance without­straining your eyes. The volume iseasy to read even when the pipetteis angled – eliminating the need toturn your head into an uncom­fortable
position.
Adjustment
Tacta® is very easy to adjust, for various
types of liquids, using a simple adjustment key. The adjustment scale shows
the degree of adjustment. By noting
this value for a specific liquid, you canreturn to that setting any time. Easy
calibration adjustment ensures accurate results with a variety of liquids.
Volume Adjustment
Use the Sartorius Optilock feature toprevent accidental volume changes
during pipetting. Either hold down thevolume lock button with one hand andadjust the volume with the other, thenrelease to lock, or with just one hand,slide the volume lock up, adjust thevolume and slide down to lock again.
The choice is in your hands.

Mechanical Pipettes Tacta®

Safe-Cone Filters
The exchangeable Safe-Cone ­Filters,
used in pipette tip cones, act asbarriers to reduce the risk of contaminating the internal components ofthe pipette. The unique Safe-Cone
Filter ejector enables the removal of
used ­filters without human contact
with contaminated filters. Safe-Cone
Filters are available for all Tacta®
models greater than 10 µL and offera cost-­effective method to reduce­contamination. Filters should bechanged regularly, and always after
over-aspiration.

Features and Benefits--

Feel the Comfort
	Comfortable to hold due to theergonomically designed handle	Low pipetting and tip ejection forcesthat reduce the risk of Work Related
Upper Limb Disorder (WRULD)
	Controlled and smooth tip ejection
with the new Sartorius Optiject­technology
	The unique Sartorius Optilock system provides flexibility for volume
adjustment and locking

21

Easy Cleaning
Tacta® is the quickest and easiest
pipette, on the market, to clean, with
only three parts to be disassembled
for cleaning and no tools are neededfor disassembly. Tacta® can also be
steam-sterilized or autoclaved as-is,
without disassembly. It has also high
UV and chemical resistance.

---

Achieve Reliable Results	Reliable results, even over lengthy
pipetting periods
	Calibration adjustment to provide
accurate results for various liquidtypes	4-digit display for accurate and easy
volume setting

Safe from Contamination	Safe-Cone Filters provide cost-­
effective contamination prevention	Easy to clean, with only three parts
to disassemble	Fully autoclavable22

Mechanical Pipettes Tacta®

Ordering Information
Tacta®
Order CodeChannels

VolumeRange

Increment

Test
VolumeSystematic Error N Limit ±Random Error N Limit

(µL)

(µL)

(µL)

(%)

(µL)

(%)

(µL)

LH-729010

1

0.1 – 3

0.002

3
1.5
0.3

1.4
2.6
10.0

0.042
0.039
0.030

0.8
1.6
6.0

0.024
0.024
0.018

LH-729020

1

0.5 – 10

0.01

10
5
1

1.0
1.5
3.0

0.100
0.075
0.030

0.6
1.0
2.0

0.060
0.050
0.020

LH-729030

1

2 – 20

0.02

20
10
2

1.0
1.4
4.0

0.200
0.140
0.080

0.5
0.9
3.0

0.100
0.090
0.060

LH-729050

1

10 – 100

0.10

100
50
10

0.8
1.0
3.0

0.80
0.50
0.30

0.2
0.4
1.0

0.20
0.20
0.10

LH-729060

1

20 – 200

0.20

200
100
20

0.6
0.8
2.3

1.20
0.80
0.46

0.2
0.3
0.9

0.40
0.30
0.18

LH-729070

1

100 – 1,000

1.00

1,000
500
100

0.7
0.8
2.5

7.0
4.0
2.5

0.2
0.2
0.6

2.0
1.0
0.6

LH-729080

1500 – 5,000

5.0

5,000
2,500
500

0.6
0.7
2.4

30
17.5
12

0.2
0.3
0.6

10
7.5
3

LH-729090

11,000 – 10,000 10.0

10,000
5,000
1,000

0.6
1.2
3.0

60
60
30

0.2
0.3
0.6

20
15
6

LH-729120
LH-729220

8120.5 – 10

0.01

10
5
1

1.5
2.5
5.5

0.150
0.125
0.055

1.0
2.0
4.0

0.100
0.100
0.040

LH-729130
LH-729230

812

5 – 100

0.10

100
50
10

0.9
1.2
4.0

0.90
0.60
0.40

0.4
0.7
2.0

0.40
0.35
0.20

LH-729140
LH-729240

8
1230 – 300

0.20

300
150
30

0.6
1.0
2.5

1.80
1.50
0.75

0.25
0.5
1.0

0.75
0.75
0.30

	Note: The listed systematic and random error values can be achieved only under strictly controlled conditions during type test per ISO 8655. The best compatibility is achieved when combining
Sartorius pipettes and Sartorius tips. The systematic error and random error results, in tests, have been achieved using Sartorius Optifit tips at factory default speed settings. Due to the continuous
product development by Sartorius, the systematic and random error values are subject to change without prior notice.

NMechanical Pipettes Tacta®

23

Tip Selection Guide
PipetteOptifit TipLRT

Color-CodeColor-Code

Safetyspace™ TipLRT
VolumeVolumeStandard

Plus

0.1 – 10 µL

0.1 – 10 µL––

0.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

0.5 – 20 µL

721014–

0.5 – 200 µL

2 – 120 µL

721008721018

0.5 – 200 µL

5 – 200 µL

721007721017

10 – 1,000 µL

50 – 1,000 µL

721006721016100 – 5,000 µL

100 – 5,000 µL

721005721015–7210057210150.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

2 – 120 µL

7210087210185 – 350 µL

5 – 300 µL

721007721017

500 – 10,000 µL

	Note: Low Retention Tips are available in volumes up to 1,200 µL.

LRTColor-CodeSafe-Code Filters–

24

Mechanical Pipettes Tacta®

Tacta® Multipacks Smart and Complete Set of­Pipettes and Accessories
Choose from a range of eight Multipacks that include a selection of Tacta®
­Mechanical Pipettes, a Linear Stand, matching Optifit Tips, and Pipette Holders.

Ordering Information
MultipackOrder Code

Tacta® Pipettes

Tacta® Pipette 3 – pack 10
LH – 729670

1-channel
0.5 – 10 µL
10 – 100 µL
100 – 1,000 µL

Tip Racks (96 tips)
0.2 – 10 µL
0.5 – 200 µL
10 – 1,000 µL

Linear Stand

1-channel
2 – 20 µL
20 – 200 µL
100 – 1,000 µL

Tip Racks (96 tips)
0.5 – 200 µL
10 – 1,000 µL

Linear Stand

1-channel
0.5 – 10 µL
2 – 20 µL
20 – 200 µL
100 – 1,000 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
10 – 1,000 µL

Linear Stand

1-channel
0.5 – 10 µL
10 – 100 µL
20 – 200 µL
100 – 1,000 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
10 – 1,000 µL

Linear Stand

1-channel
0.5 – 10 µL
10 – 100 µL
20 – 200 µL
100 – 1,000 µL
500 – 5,000 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
10 – 1,000 µL
Tip Rack (50 tips)
100 – 5,000 µL

Linear Stand

1-channel
2 – 20 µL
10 – 100 µL
20 – 200 µL
100 – 1,000 µL
500 – 5,000 µL

Tip Racks (96 tips)
0.5 – 200 µL
10 – 1,000 µL
Tip Rack (50 tips)
100 – 5,000 µL

Linear Stand

Tacta® Pipette 3+1 – pack
LH – 729676

1-channel
0.5 – 10 µL
20 – 200 µL
100 – 1,000 µL
8-channel
30 – 300 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
5 – 350 µL
10 – 1,000 µL

Linear Stand

Tacta® Pipette 3 – pack max
LH – 729677

1-channel
100 – 1,000 µL
500 – 5,000 µL
1,000 – 10,000 µL

Tip Racks
10 – 1,000 µL (96 tips)
100 – 5,000 µL (50 tips)
500 – 10,000 µL (35 tips)

Linear Stand

Tacta® Pipette 3 – pack 20
LH – 729671

Tacta® Pipette 4 – pack 20
LH – 729672

Tacta® Pipette 4 – pack 100
LH – 729673

Tacta® Pipette 5 – pack 10
LH – 729674

Tacta® Pipette 5 – pack 20
LH – 729675-------------

Optifit Tips----------

Accessories

26

Mechanical Pipettes

Mline®

Mline® Mechanical Pipettes
Effortless Accuracy

Mline® low pipetting forces­ rotect laboratory workers fromp
injury.

No rocking needed

Optiload secures even tip sealing
onto every individual tip cone,
and allows tips to be loaded andejected with minimum force.

Sartorius’ mechanical pipette family –the Mline® – offers excellent ergonomics, performance and safety in manual
pipetting. It is designed to maintainhigh accuracy and precision in repetitive and long-lasting manual pipetting.
Its excellent ergonomics minimize
the risk of work related hand, arm andshoulder disorders and Work Related
Upper Limb Disorder (WRULD).
It covers the full volume range of 0.1 µL
to 10 mL and is available in single- andmulti-channel models.
Excellent Ergonomics with
Low Pipetting Forces
Excellent ergonomics and low pipetting forces protect laboratory workers
from injury. Mline® has exceptionallylight pipetting and tip ejection forces
due to its patented spring mechanism.
The light pipetting force improves
pipetting precision in long pipetting
series.
The starting forces in Mline® pipettes
are always constant, regardless of theset volume. This improves pipetting
results especially for small volumes.

Safe-Cone Filters protect thepipette from contamination, andshould be changed regularly.

Optiload – Loading Tips with
­Minimum Force
Mline® pipettes have spring-loaded tip
cones – the Optiload mechanism – onboth single and multi-­channel models.
Optiload secures even tip sealing onto
every individual tip cone, and allows
tips to be loaded and ­ejected with minimum force. This is an advantage especially when ­working with multi-channel
models that ­otherwise would require
more force for tip loading and ejection
than a single-channel model.
Safe-Cone Filters Protect
the Pipette
The replaceable Safe-Cone Filter
located inside the tip-cone preventsaerosols and fluids from penetrating
the pipette, also in cases of over-­aspiration. The use of Safe-Cone
Filters lengthens the maintenanceinterval of the pipette.
Safe-Cone Filters are available for all
Mline® models greater than 10 µL.
They must be replaced regularly, andalways in cases of over-aspiration.
Safe-Cone Filters can easily and safely
be ejected by removing the color
cap and pressing down the operating
button.

Mechanical Pipettes

Mline®

Features and Benefits--	Low pipetting forces that prevent
WRULD and improve results in long
pipetting series	Ergonomic finger support minimizes
the grip force needed to hold thepipette	Optiload mechanism in both singleand multi-channel models for easy
and light tip loading with perfect tip
sealing	Single and multi-channel models
	Volume adjustment locking for­preventing accidental volume
changes27---	Big volume display
	Color-coding of volumes to ease theselection of corresponding pipettetips	Safe-Cone Filters available formodels > 10 µL with convenient filter
ejection mechanism	Fully autoclavable without­disassembly	Simple to clean and maintain with
only three parts to disassemble	Materials have high chemical and
UV-resistance to ensure a long life
span for the pipette

28

Mechanical Pipettes

Mline®

Ordering Information
Mline®
Order CodeChannels

VolumeRange

Increment

Test
VolumeSystematic Error N Limit ±Random Error N Limit

(µL)

(µL)

(µL)

(%)

(µL)

(%)

(µL)

7250101

0.1 – 3

0.002

3
1.5
0.3

1.4
2.6
10.0

0.042
0.039
0.030

0.8
1.6
6.0

0.024
0.024
0.018

7250201

0.5 – 10

0.01

10
5
1

1.0
1.5
3.0

0.100
0.075
0.030

0.6
1.0
2.0

0.060
0.050
0.020

7250301

2 – 20

0.02

20
10
2

1.0
1.4
4.0

0.200
0.140
0.080

0.5
0.9
3.0

0.100
0.090
0.060

7250501

10 – 100

0.10

100
50
10

0.8
1.0
3.0

0.80
0.50
0.30

0.2
0.4
1.0

0.20
0.20
0.10

7250601

20 – 200

0.20

200
100
20

0.6
0.8
2.3

1.20
0.80
0.46

0.2
0.3
0.9

0.40
0.30
0.18

7250701

100 – 1,000

1.00

1,000
500
100

0.7
0.8
2.5

7.0
4.0
2.5

0.2
0.2
0.6

2.0
1.0
0.6

7250801

500 – 5,000

10.0

5,000
2,500
500

0.6
0.7
2.4

30
17.5
12

0.2
0.3
0.6

10
7.5
3

7250901

1,000 – 10,000 20.0

10,000
5,000
1,000

0.6
1.2
3.0

60
60
30

0.2
0.3
0.6

20
15
6

7251207252208
12

0.5 – 10

0.01

10
5
1

1.5
2.5
5.5

0.150
0.125
0.055

1.0
2.0
4.0

0.100
0.100
0.040

7251307252308
12

5 – 100

0.10

100
50
10

0.9
1.2
4.0

0.90
0.60
0.40

0.4
0.7
2.0

0.40
0.35
0.20

7251407252408
1230 – 300

0.20

300
150
30

0.6
1.0
2.5

1.80
1.50
0.75

0.25
0.5
1.0

0.75
0.75
0.30

	Note: The listed systematic and random error values can be achieved only under strictly controlled conditions during type test per ISO 8655. The best compatibility is achieved when combining
Sartorius pipettes and Sartorius tips. The systematic error and random error results, in tests, have been achieved using Sartorius Optifit tips at factory default speed settings. Due to the continuous
product development by Sartorius, the systematic and random error values are subject to change without prior notice.

NMechanical Pipettes

Mline®

29

Tip Selection Guide
PipetteOptifit TipLRT

Color-CodeColor-Code

Safetyspace™ TipLRT
VolumeVolumeStandard

Plus

0.1 – 10 µL

0.1 – 10 µL––

0.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

0.5 – 20 µL

721014–

0.5 – 200 µL

2 – 120 µL

721008721018

0.5 – 200 µL

5 – 200 µL

721007721017

10 – 1,000 µL

50 – 1,000 µL

721006721016100 – 5,000 µL

100 – 5,000 µL

721005721015–7210057210150.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

2 – 120 µL

7210087210185 – 350 µL

5 – 300 µL

721007721017

500 – 10,000 µL

	Note: Low Retention Tips are available in volumes up to 1,200 µL.

LRTColor-CodeSafe-Code Filters–

30

Mechanical Pipettes

Mline®

Mline® Multipacks – Complete Sets of Pipettes
and Accessories
Mline® Multipacks offer sets of mechanical pipettes in an affordable package,
including a Linear Stand and racks of matching tips.

Ordering Information
MultipackOrder Code

Mline® Pipettes

Mline® Pipette 3-pack 10
LH-725661

1-channel
0.5 – 10 µL
10 – 100 µL
100 – 1,000 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
10 – 1,000 µL

Linear Stand

1-channel
2 – 20 µL
20 – 200 µL
100 – 1,000 µL

Tip Racks (96 tips)
0.5 – 200 µL
10 – 1,000 µL

Linear Stand

1-channel
0.5 – 10 µL
10 – 100 µL
20 – 200 µL
100 – 1,000 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
10 – 1,000 µL

Linear Stand

1-channel
2 – 20 µL
10 – 100 µL
20 – 200 µL
100 – 1,000 µL
500 – 5,000 µL

Tip Racks (96 tips)
0.5 – 200 µL
10 – 1,000 µL
Tip Rack (50 tips)
5,000 µL

Linear Stand

1-channel
0.5 – 10 µL
20 – 200 µL
100 – 1,000 µL
8-channel
30 – 300 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
5 – 350 µL
10 – 1,000 µL

Linear Stand

Mline® Pipette 3-pack 20
LH-725662

Mline® Pipette 4-pack
LH-725663

Mline® Pipette 5-pack
LH-725664

Mline® Pipette 3+1-pack
LH-725665--------

Sartorius Optifit Tips-------

Accessories

32

Mechanical Pipettes

Proline® Plus

Proline® Plus Mechanical Pipettes
Dependable Durability

Fixed volume Proline® Plus pipette

Proline® Plus has a comfortablehandle and an ergonomic finger
support for effortless pipetting.

Safe-Cone Filters protect thepipette from contamination, andshould be changed regularly.

The Proline® Plus mechanical ­pipettefamily is designed to offer comfort and quality for your ­everyday­manual ­pipetting. It combines durable ­construction with ease andlightness of use, and is therefore theperfect choice for liquid-handlingpro­fessionals and students alike. In­addition, it has the widest pipetterange, including fixed volume pipettes
for when volumes need to be readyset to avoid errors.
Ergonomic Design
Proline® Plus has low pipetting forces,
a comfortable handle and an ergonomic finger support for effortless
pipetting. Good fit in hand minimizes
the grip force needed to hold thepipette, thereby reducing the risk ofstrain injury.
Loading Tips on Multi-channel Pipettes with Minimum Force
Loading and ejecting tips with multi-­
channel pipettes requires relatively
high forces. Proline® Plus multi-­
channel pipettes have spring loaded
tip cones – the Optiload mechanism.
Optiload allows tips to be loaded andejected with minimum force, which­reduces the risk of hand injuries.
­Moreover, it secures even tip sealing
onto every individual tip cone.

Protect the Pipette with
Safe-Cone Filters
The replaceable Safe-Cone Filter
located inside the tip-cone preventsaerosols and fluids from penetrating
the pipette, also in case of over-­aspiration. The use of Safe-Cone
Filters lengthens the maintenanceinterval of the pipette. Safe-Cone
Filters are available for all Proline® Plusmodels greater than 10 µL. They must
be replaced regularly, and always incase of overaspiration.
Easy Maintenance and Adjustment
No opening tools are needed forcleaning and maintaining Proline® Plus
pipettes, and only three parts need to
be cleaned. These pipettes are alsoeasy to adjust using the adjustment
tool that is provided with the pipette.

Mechanical Pipettes

Proline® Plus

Features and Benefits

Proline® Plus pipettes have only
three parts that need cleaning andmaintenance.

-	Low pipetting forces that prevent
WRULD and improve results in long
pipetting series	Ergonomic finger support minimizes
the grip force needed to hold thepipette	Optiload mechanism in multi-­
channel models for easy and light tip
loading with perfect tip sealing	Wide variety of adjustable single andmulti-channel models as well as fixed
single-channel models
	Volume range from 3 µL (5 µL for
fixed) to 10 mL

33

----

	Easy volume setting with click stopmechanism	Big volume display
	Color-coding of different volumes
to ease the selection of matching
pipette tips
	Safe-Cone Filters available for­models > 10 µL
	Fully autoclavable without­disassembly	Simple to clean and maintain with
only three parts to disassemble	Materials have high chemicaland UV-resistance to secure long­life-time of the pipette

34

Mechanical Pipettes

Proline® Plus

Ordering Information
Proline® Plus
Order CodeChannels

VolumeRange

Increment

Test
VolumeSystematic Error N Limit ±Random Error N Limit

(µL)

(µL)

(µL)

(%)

(µL)

(%)

(µL)

7280101

0.1 – 3

0.002

3
1.5
0.3

1.4
2.6
10.0

0.042
0.039
0.030

0.8
1.6
6.0

0.024
0.024
0.018

7280201

0.5 – 10

0.01

10
5
1

1.0
1.5
3.0

0.100
0.075
0.030

0.6
1.0
2.0

0.060
0.050
0.020

7280301

2 – 20

0.02

20
10
2

1.0
1.4
4.0

0.200
0.140
0.080

0.5
0.9
3.0

0.100
0.090
0.060

7280401

5 – 50

0.10

50
25
5

1.0
1.4
3.0

0.500
0.350
0.150

0.3
0.5
1.5

0.150
0.125
0.075

7280501

10 – 100

0.10

100
50
10

0.8
1.0
3.0

0.80
0.50
0.30

0.2
0.4
1.0

0.20
0.20
0.10

7280601

20 – 200

0.20

200
100
20

0.6
0.8
2.3

1.20
0.80
0.46

0.2
0.3
0.9

0.40
0.30
0.18

7280701

100 – 1,000

1.00

1,000
500
100

0.7
0.8
2.5

7.0
4.0
2.5

0.2
0.2
0.6

2.0
1.0
0.6

7280801

500 – 5,000

10.0

5,000
2,500
500

0.6
0.7
2.4

30
17.5
12

0.2
0.3
0.6

10
7.5
3

7280901

1,000 – 10,000 20.0

10,000
5,000
1,000

0.6
1.2
3.0

60
60
30

0.2
0.3
0.6

20
15
6

7281207282208
12

0.5 – 10

0.01

10
5
1

1.5
2.5
5.5

0.150
0.125
0.055

1.0
2.0
4.0

0.100
0.100
0.040

7281307282308
12

10 – 100

0.10

100
50
10

0.9
1.2
4.0

0.90
0.60
0.40

0.4
0.7
2.0

0.40
0.35
0.20

7281407282408
1230 – 300

0.20

300
150
30

0.6
1.0
2.5

1.80
1.50
0.75

0.25
0.5
1.0

0.75
0.75
0.30

	Note: The listed systematic and random error values can be achieved only under strictly controlled conditions during type test per ISO 8655. The best compatibility is achieved when combining
Sartorius pipettes and Sartorius tips. The systematic error and random error results, in tests, have been achieved using Sartorius Optifit tips at factory default speed settings. Due to the continuous
product development by Sartorius, the systematic and random error values are subject to change without prior notice.

NMechanical Pipettes

Proline® Plus

35

Tip Selection Guide
PipetteOptifit TipLRT

Color-CodeColor-Code

Safetyspace™ TipLRT
VolumeVolumeStandard

Plus

0.1 – 10 µL

0.1 – 10 µL––

0.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

0.5 – 20 µL

721014–

0.5 – 200 µL

2 – 120 µL

721008721018

0.5 – 200 µL

2 – 120 µL

721008721018

0.5 – 200 µL

5 – 200 µL

721007721017

10 – 1,000 µL

50 – 1,000 µL

721006721016100 – 5,000 µL

100 – 5,000 µL

721005721015–7210057210150.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

2 – 120 µL

7210087210185 – 350 µL

5 – 300 µL

721007721017

500 – 10,000 µL

	Note: Low Retention Tips are available in volumes up to 1,200 µL.

LRTColor-CodeSafe-Code Filters–

36

Mechanical Pipettes

Proline® Plus

Ordering Information

Proline® Plus FIXED Volume, Single Channel
Order CodeChannels

VolumeTest Volume

Systematic Error N Limit ±Random Error N Limit

(µL)

(µL)

(%)

(µL)

(%)

(µL)

728515

1

5

5

1.3

0.065

1.2

0.060

728520

1

10

10

0.8

0.080

0.8

0.080

728530

1

20

20

0.6

0.120

0.5

0.100

728535

1

25

25

0.5

0.125

0.3

0.075

728545

1

50

50

0.5

0.250

0.3

0.150

728550

1

100

100

0.5

0.50

0.3

0.30

728560

1

200

200

0.4

0.80

0.2

0.40

728565

1

250

250

0.4

1.00

0.2

0.50

728567

1

500

500

0.3

1.50

0.2

1.00

728570

1

1,000

1,000

0.3

3.0

0.2

2.0

728575

1

2,000

2,000

0.3

6.0

0.15

3.0

728580

1

5,000

5,000

0.3

15

0.15

7.5

7285901

10,00010,000

0.6

60

0.2

20

	Note: The listed systematic and random error values can be achieved only under strictly controlled conditions during type test per ISO 8655. The best compatibility is achieved when combining
Sartorius pipettes and Sartorius tips. The systematic error and random error results, in tests, have been achieved using Sartorius Optifit tips at factory default speed settings. Due to the continuous
product development by Sartorius, the systematic and random error values are subject to change without prior notice.

NMechanical Pipettes

Proline® Plus

37

Tip Selection Guide

PipetteOptifit TipLRT

Color-CodeColor-Code

Safetyspace™ TipLRT
VolumeVolumeStandard

Plus

0.1 – 10 µL

0.1 – 10 µL––

0.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

0.5 – 20 µL

721014–

0.5 – 200 µL

2 – 120 µL

721008721018

0.5 – 200 µL

2 – 120 µL

721008721018

0.5 – 200 µL

2 – 120 µL

721008721018

0.5 – 200 µL

5 – 200 µL

721007721017

10 – 1,000 µL

50 – 1,000 µL

72100672101610 – 1,000 µL

50 – 1,000 µL

72100672101610 – 1,000 µL

50 – 1,000 µL

721006721016100 – 5,000 µL

100 – 5,000 µL

721005721015100 – 5,000 µL

100 – 5,000 µL

721005721015721005721015500 – 10,000 µL

Color-CodeSafe-Code Filters

	Note: Low Retention Tips are available in volumes up to 1,200 µL.

LRTProline® Plus Fixed Volume Pipettes

38

Mechanical Pipettes

Proline® Plus

Proline® Plus Multipacks – Complete Sets of Pipettes
and Accessories
Proline® Plus Multipacks offer aresets of mechanical pipettes in an­affordable package, including a Linear
Stand and racks of matching tips.

These Multipacks allow the affordable
renewal of existing pipettes, or to set
up new workstations, with a set of
highly ergonomic pipettes with highaccuracy and precision.

Ordering Information
MultipackOrder Code

Proline® Plus Pipettes

Proline® Plus Pipette 3-pack 10
LH-728670

1-channel
0.5 – 10 µL
10 – 100 µL
100 – 1,000 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
10 – 1,000 µL

Linear Stand

1-channel
2 – 20 µL
20 – 200 µL
100 – 1,000 µL

Tip Racks (96 tips)
0.5 – 200 µL
10 – 1,000 µL

Linear Stand

1-channel
0.5 – 10 µL
2 – 20 µL
20 – 200 µL
100 – 1,000 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
10 – 1,000 µL

Linear Stand

1-channel
0.5 – 10 µL
10 – 100 µL
20 – 200 µL
100 – 1,000 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
10 – 1,000 µL

Linear Stand

1-channel
0.5 – 10 µL
10 – 100 µL
20 – 200 µL
100 – 1,000 µL
500 – 5,000 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
10 – 1,000 µL
Tip Rack (50 tips)
100 – 5,000 µL

Linear Stand

1-channel
2 – 20 µL
10 – 100 µL
20 – 200 µL
100 – 1,000 µL
500 – 5,000 µL

Tip Racks (96 tips)
0.5 – 200 µL
10 – 1,000 µL
Tip Rack (50 tips)
100 – 5,000 µL

Linear Stand

Proline® Plus Pipette 3+1-pack
LH-728676

1-channel
0.5 – 10 µL
20 – 200 µL
100 – 1,000 µL
8-channel
30 – 300 µL

Tip Racks (96 tips)
0.1 – 10 µL
0.5 – 200 µL
5 – 350 µL
10 – 1,000 µL

Linear Stand

Proline® Plus Pipette 3-pack max
LH-728677

1-channel
100 – 1,000 µL
500 – 5,000 µL
1,000 – 10,000 µL

Tip Racks
10 – 1,000 µL (96 tips)
100 – 5,000 µL (50 tips)
500 – 10,000 µL (35 tips)

Linear Stand

Proline® Plus Pipette 3-pack 20
LH-728671

Proline® Plus Pipette 4-pack 20
LH-728672

Proline® Plus Pipette 4-pack 100
LH-728673

Proline® Plus Pipette 5-pack 10
LH-728674

Proline® Plus Pipette 5-pack 20
LH-728675-------------

Sartorius Optifit Tips----------

Accessories

Mechanical Pipettes

Proline®

Proline® Mechanical P­ ipettes
Affordable Reliability

The fact that Proline®, our first­mechanical pipette, is still in use inmany laboratories by over 200,000­users, testifies to its timeless, ­practicaldesign and reliability. Being themost affordable pipette in Sartorius’
range of mechanical pipettes, it isideal for universities and colleges, or
any l­aboratory seeking a cost-efficientliquid handling tool.
Due to its relatively light weight, highaccuracy and precision, it is used bymany professionals.

Features and Benefits---	Wide range of pipettes, both
­adjustable and fixed volume
	Volume range from 2,5 µL (5 µL for
fixed) to 5 mL	Easy volume setting with click stopmechanism
	Safe-Cone Filters available for­models > 10 µL

39

40

Mechanical Pipettes

Proline®

Ordering Information
Proline®
Order CodeChannels

VolumeRange

Increment

Test
VolumeSystematic Error N Limit ±Random Error N Limit

(µL)

(µL)

(µL)

(%)

(µL)

(%)

(µL)

7200101

0.1 – 2.5

0.05

2.5
1.25
0.25

2.5
3.0
12.0

0.063
0.038
0.036

2.0
3.0
6.0

0.050
0.038
0.018

7200151

0.5 – 10

0.10

10
5
1

1.0
1.5
3.0

0.100
0.075
0.030

0.8
1.5
2.0

0.080
0.075
0.020

7200801

2 – 20

0.50

20
10
2

1.0
1.4
4.0

0.200
0.140
0.080

0.5
0.9
3.0

0.100
0.090
0.060

7200251

5 – 50

0.50

50
25
5

1.0
1.4
3.0

0.500
0.350
0.150

0.3
0.6
2.0

0.150
0.150
0.100

7200501

10 – 100

1.00

100
50
10

0.8
1.0
3.0

0.80
0.50
0.30

0.2
0.4
1.0

0.20
0.20
0.10

7200701

20 – 200

1.00

200
100
20

0.6
0.8
2.5

1.20
0.80
0.50

0.2
0.3
0.9

0.40
0.30
0.18

7200601

100 – 1,000

5.00

1,000
500
100

0.7
0.8
2.5

7.0
4.0
2.5

0.2
0.25
0.7

2.0
1.25
0.7

7201101

1,000 – 5,000

50.0

5,000
2,500
1,000

0.6
0.7
1.2

30
17.5
12

0.2
0.3
0.3

10
7.5
3

7202107203108
12

0.5 – 10

0.10

10
5
1

1.5
2.5
5.5

0.150
0.125
0.055

1.5
2.5
4.0

0.150
0.125
0.040

7202207203208
12

5 – 50

0.50

50
25
5

1.0
1.5
3.0

0.500
0.375
0.150

0.5
1.0
2.0

0.250
0.250
0.100

7202407203408
1250 – 300

5.00

300
150
50

0.7
1.0
1.5

2.10
1.50
0.75

0.25
0.5
0.8

0.75
0.75
0.40

	Note: The listed systematic and random error values can be achieved only under strictly controlled conditions during type test per ISO 8655. The best compatibility is achieved when combining
Sartorius pipettes and Sartorius tips. The systematic error and random error results, in tests, have been achieved using Sartorius Optifit tips at factory default speed settings. Due to the continuous
product development by Sartorius, the systematic and random error values are subject to change without prior notice.

NMechanical Pipettes

Proline®

41

Tip Selection Guide
PipetteOptifit TipLRT

Color-CodeColor-Code

Safetyspace™ TipLRT
VolumeVolumeStandard

Plus

0.1 – 10 µL

0.1 – 10 µL––

0.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

0.5 – 20 µL

721014–

0.5 – 200 µL

2 – 120 µL

721008721018

0.5 – 200 µL

2 – 120 µL

721007721017

0.5 – 200 µL

5 – 200 µL

721007721017

10 – 1,000 µL

50 – 1,000 µL

721006721016100 – 5,000 µL

100 – 5,000 µL

7210057210150.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

2 – 120 µL

721014–5 – 350 µL

5 – 300 µL

721014–	Note: Low Retention Tips are available in volumes up to 1,200 µL.

LRTColor-CodeSafe-Code Filters

42

Mechanical Pipettes

Proline® Plus

Ordering Information

Proline® Plus FIXED Volume, Single Channel
Order CodeChannels

VolumeTest Volume

Systematic Error N Limit ±Random Error N Limit

(µL)

(µL)

(%)

(µL)

(%)

(µL)

722001

1

5

5

1.3

0.065

1.2

0.060

722004

1

10

10

0.8

0.080

0.8

0.080

722010

1

20

20

0.6

0.120

0.5

0.100

722015

1

25

25

0.5

0.125

0.3

0.075

722020

1

50

50

0.5

0.250

0.3

0.150

722025

1

100

100

0.5

0.50

0.3

0.30

722030

1

200

200

0.4

0.80

0.2

0.40

722035

1

250

250

0.4

1.00

0.2

0.50

722040

1

500

500

0.3

1.50

0.2

1.00

722045

1

1,000

1,000

0.3

3.0

0.2

2.0

722050

1

2,000

2,000

0.3

6.0

0.15

3.0

722055

1

5,000

5,000

0.3

15

0.15

7.5

	Note: The listed systematic and random error values can be achieved only under strictly controlled conditions during type test per ISO 8655. The best compatibility is achieved when combining
Sartorius pipettes and Sartorius tips. The systematic error and random error results, in tests, have been achieved using Sartorius Optifit tips at factory default speed settings. Due to the continuous
product development by Sartorius, the systematic and random error values are subject to change without prior notice.

NMechanical Pipettes

Proline® Plus

43

Tip Selection Guide

PipetteOptifit TipLRT

Color-CodeColor-Code

Safetyspace™ TipLRT
VolumeVolumeStandard

Plus

0.1 – 10 µL

0.1 – 10 µL––

0.1 – 10 µL

0.1 – 10 µL––

0.5 – 200 µL

0.5 – 20 µL

721014–

0.5 – 200 µL

0.5 – 20 µL

721008721018

0.5 – 200 µL

0.5 – 20 µL

721008721018

0.5 – 200 µL

2 – 120 µL

721007721017

0.5 – 200 µL

5 – 200 µL

721007721017

10 – 1,000 µL

50 – 1,000 µL

72100672101610 – 1,000 µL

50 – 1,000 µL

72100672101610 – 1,000 µL

50 – 1,000 µL

721006721016100 – 5,000 µL

100 – 5,000 µL

721005721015100 – 5,000 µL

100 – 5,000 µL

721005721015

	Note: Low Retention Tips are available in volumes up to 1,200 µL.

LRTColor-CodeSafe-Code Filters

45Stands and Accessories
Table of Contents

Stands and Accessories
Pipette Stands
Elbow Pad
Safe-Cone Filters
Reagent Vessel
Adjustment Tool

46
47
48
50
50

46

Stands and Accessories

Pipette Stands

Pipette Stands

Charging Carousel Stand

When the pipette is not in use, it
should be stored in an upright ­positionin order to avoid contamination fromwork surfaces. Sartorius provides
stands for all of its pipettes. It is recommended that electronic pipettes
be stored and charged on a charging
stand whenever they are not in use. In
this way, their batteries always remain
charged for when work begins.

The Linear Stand is designed for all
Sartorius mechanical and electronic
pipettes. This stand is also compatible
with a wide range of other pipettemakes.
The simplest of all are the pipetteholders which are attached to thefront edge of a shelf. These are suitable
for mechanical pipettes.

Compact carousel stands are ideal forsaving bench space. There is one formechanical pipettes, and a charging
carousel stand for electronic pipettes.

Ordering Information
Pipette Stands
Charging Stand

Order Code

Item

730981

Charging Stand for one electronic pipette*

730991

Charging Carousel for 4 electronic pipettes*

725620Linear Stand for all Sartorius pipette models

LH-725630

Carousel Stand for 6 mechanical pipettes

LH-727650

Adapter for Mechanical Carousel Stand

LH-727640

Holder for one pipette* Supplied with a universal charger (EU, UK, US | JPN, AUS, KOR and CHN plugs)

Linear Stand (non-charging)

Pipette Holder for one Pipette

Carousel Stand (non-charging)

Adapter for Mechanical
Carousel StandStands and Accessories

Elbow Pad

Elbow Pad
The Elbow Pad provides comfort while
pipetting. The visco-elastic material of
the pad relieves contact stress, painand discomfort under the elbow.

Features and Benefits---	Improves pipetting ergonomics
	Conforms to any elbow size or shape	Coating is pleasant to the skin	The compact size takes up little
bench space

47--

The Elbow Pad is Ideal for	long periods of pipetting	work requiring high concentration,e.g. micro plate work	any work where a cushion beneaththe elbow or wrist is needed--	Very durable
	Easy to clean with washing up liquid,or ethanol (70 %)	Not autoclavable

Ordering Information
Elbow Pad
Order Code

Item

Qty

723103

Elbow Pad1

48

Stands and Accessories

Safe-Cone Filters

Safe-Cone Filters
Why Should You Use Safe-Cone
Filters?
These unique and replaceable polyethylene (PE) filters prevent any fluids
and liquid vapours from reaching theinternal components of the pipette.

---	Reduce the risk of contaminating
the internal components of ourpipettes	Prolong the pipette’s lifetime
	Reduce maintenance intervals
	Are cost-effective compared tofilter tips

When Should You Use them?
The ultimate pipette protectors areavailable in two types:

Built-in filter ejector in Tacta®

Plus Filter
For more demanding applications
such as radioactive work, cell culture,bacterial and virological work andmolecular biology.

How Often Should You Change?
The interval of changing the­filter ­depends completely on the­application and the sample. ­However,according to studies, the filter is­recommended to be changed ­daily(after 50 to 250 pipettings) and­immediately in case of over-aspi­ration.
How to Change?
To ensure that the user is protectedfrom contamination, tweezers should
be used when removing used filtersfrom the pipette tip cone. The Tacta®
and the Mline® feature a built-in filter
ejectors, so tweezers are unnecessary.
In addition, the tip cone should becleaned with ethanol (70 %) prior to
the insertion of a new filter.

Standard Filter
For general applications. Can beused in same type of work as the Plusfilter, but needs to be changed more
frequently.

Tweezers for exchanging filters in pipettes are supplied with all pipettes
excluding Tacta® and Mline®.

Stands and Accessories

Safe-Cone Filters

49

Contamination in Pipette Interior
Filter

No filter

8,000
7,000

Bacterial Growth

6,000
5,000
4,000
3,000
2,000
1,000
0

10 ×

100 ×

250 ×

500 ×

Number of pipettings
Pipette contamination in pipette interior when pipetting liquid culture ofbacteria Micrococcus Luteus.

Ordering Information
Safe-Cone Filters
Order CodeItem

Qty / Unit

721008

Standard ∅ 2.51 mm PE

50

721007

Standard ∅ 3.15 mm PE

50

721006

Standard ∅ 5.33 mm PE

50

721005

Standard ∅ 6.73 mm PE

50

721014Standard ∅ 1.83 mm Polyolefin

50

721018Plus ∅ 2.51 mm PE

50

721017

Plus ∅ 3.15 mm PE

50

721016Plus ∅ 5.33 mm PE

50

721015Plus ∅ 6.73 mm PE

50

721009Tweezers for Safe-Cone Filters

1

PE = polyethylene
See the pipette ordering information charts for corresponding filters and pipettes.

50Stands and Accessories

Reagent Vessel | Calibration Tool

Reagent Vessel
Made from polypropylene, the autoclavable and durable reagent vessel
is chemically resistant to all common
reagents.

Ordering Information
Reagent Vessel
Order Code

Item

Qty

783500

Reagent Vessel (capacity 120 mL)

16

Adjustment Tool
The Adjustment Tool is used to adjust
the pipette in situations where thefactory calibration is not applicable.

Ordering Information
Adjustment Tool
Adjustment Tool is used for­adjusting Mline® and Proline® Plus
pipettes.

The Adjustment Tool for adjusting
the Tacta® pipette.

Order Code

Item

Qty

726203Adjustment Tool for Mline® | Proline® Plus

1

LH-727080

Adjustment Tool for Tacta

1721130Adjustment Tool for Proline

®
®

1

53

Pipette Tips
Table of Contents

Pipette Tips
Pipette Tips
Optifit Tips
Safetyspace™ Filter Tips
Low Retention Tips
Packaging Options

54
56
57
58
60

54

Pipette Tips

Optifit Tips and Safetyspace™ Filter Tips

Pipette Tips

The Perfect Match for Your Pipette

Optiload for a tight fit and equalsealing on every channel’s tip cone

Sartorius pipette tips ensure theperformance of Sartorius pipettesand ­repeatability of your results
like no ­other tip can. They havebeen ­designed and manufactured
to meet the highest quality and­purity s­ tandards and to be the inert­protectors of your pipette. ­Moreover,­correctly fitting tips protect the­pipette’s tip cone from wear and tear.
Sartorius tip packages are ­designed tomake the daily work of lab professionals easier. Our offering ­covers a variety
of functional tip ­package options withvarious purity ratings.

The high purity and consistent ­qualityof Sartorius tips provide your valuable
samples with the ultimate protection
from contamination. We adhere
to strict quality standards and ­control
procedures – from raw ­material
to automated manufacturing and­packaging.
Sartorius tips correspond with thecolor-coding of Sartorius pipettes, toallow easy matching of corresponding
volumes.

Premium Quality and Purity
Manufacturing the tips in our own
production facility allows us to maintain the highest quality and purity
standards, by selecting the best plastic
materials and controlling the manufacturing process from beginning to end.

Contamination Free Tips

Certificate of Release

Product Description:

Filter Tip: 50 – 1000 µL

Reference:

LH-LF791001

Batch number:

500970116

Expiry date:

07/2025

Specifications
The product is certified to be DNase, RNase, human DNA andendotoxin free and is tested by an independent laboratory.
Statements:
Endotoxins:
Sample preparation was carried out according to FDA guidelines for
medical devices by extracting the pipette tips cut into pieces for
1 hour at 37°C with an optimized volume of pyrogen-free water.
Endotoxins in the extractions were detected with Chromogenic
kinetic method according to Ph.Eur. 2.6.14, Method D, which isbased on development of colour after cleavage of a synthetic
peptide-chromogen complex. Validated test result for endotoxin-freepipette tips is < 0.005 IU/mL (EU/mL).

The sterilization process is electron beam (beta irradiation) inaccordance with EN 552 and ISO 11137 and is validated by using
25 kGy as the minimum dose with SAL (sterility assurance level)of 10-6.
This is to certify that the product described has been manufactured
in accordance with ISO 9001:2015, Certificate No. FI17/5101 andISO 13485:2016, Certificate No. FI17/5103 quality management
protocols and has passed all relevant Quality Assurance procedures
related to this product.
The expiry date of the product is 5 years from the manufacturing
date and is stated on the product label of the packaging.

RNase
The tips were pipetted up and down in an optimized volume of purewater using maximal pipette tip capacity. RNase activity in theextraction was measured with fluorometric assay by detecting
degradation of labelled RNase substrate. RNase-free pipette tips
show no evidence of RNase activity in the assay with the detectionlevel of < 3,125*10-9 U/µL when RNase A was used as a standard.
DNase
The tips were pipetted up and down in an optimized volume of purewater using maximal pipette tip capacity. DNase activity in theextraction was measured with fluorometric assay by detecting
degradation of labelled DNase substrate. DNase-free pipette tips
show no evidence of DNase activity in the assay with the detectionlevel of < 6,25*10-5 U/µL when DNase I was used as a standard.
Human DNA:The tips were pipetted up and down in an optimized volume of purewater using maximal pipette tip capacity.
The sample, positive and negative controls and a dilution series ofstock DNA were analyzed in quantitative PCR (qPCR) using humanDNA specific primers. The analysis shows no evidence of human
DNA in the assay with the detection level of < 1 pg/μL.

27. August 2020
Date of Release

Our quality management system follows not only ISO 9001 and ISO 14001,
but also ISO 13485. Tip production also
abides by the ISO 14644-1 standard,in order to fulfil ISO Class 8 cleanroom
conditions for secured tip purity.

Seppo Riikonen
Quality Manager Pipettes and Dispensing
Manufactured by: Sartorius Biohit Liquid
Handling Oy, Head Office: Laippatie 1,00880 Helsinki, Finland
Phone +358 9 755 951
Contact: lhinfo.finland@sartorius.com
www.sartorius.com

Lot-specific purity certificate

To avoid contamination throughhuman contact, we have automatedthe entire tip manufacturing process.
Pure virgin polypropylene (PP) plastic
is automatically fed from silos into
molding machines. Molding machines
and robots located in isolated clean
cells, load the tips automatically into
tip trays and packaging. HEPA filtersand higher air pressure are applied
for purity within the cell. Sartorius­Single Tray tip racks, Single Refill Packs
and FlexiBulk® packs are individually
and automatically packed in air-tight­plastic, in order to rule out any dangerof contamination.

Additionally, our highly experienced
and trained personnel are equippedwith specially designed coveralls,
masks, hair nets and gloves, in order tofurther diminish risks of contamination.
An independent laboratory checks
every tip lot of Single Tray and Refill
Pack for DNase, RNase, human DNA
and endotoxins. Lot-specific purity
certificates can be downloaded fromwww.sartorius.com.

Pipette Tips

Optifit Tips and Safetyspace™ Filter Tips

55

Definitions
DNase

A deoxyribonuclease (DNase) is any enzyme that catalyzes the­degradation of DNA. The absence of DNase is tested by using­fluorometric assay. The detection level of the assay is 6.25* 10-5 U/µL
when DNase I is used as a standard.

RNase

Ribonuclease (RNase) is an enzyme that catalyzes the degradationof RNA into smaller components and can be generally found from­organisms. The absence of RNase is tested by using fluorometric assay.
The detection level of the assay is 3.125* 10-9 U/µL, when RNase A is
used as a standard.

Endotoxins

Endotoxins are lipopolysaccharides found in the Gram-negative bacteria
and can cause several serious health effects in humans and animals.
Limulus Amebocyte Lysate (LAL) Gel Clot method is used to detect thepresence of endotoxins on the pipette tips. The detection level of the
LAL assay is 0.005 IU/mL (EU/mL).

Sterilization

The destruction of all microbial life, including bacterial endospores.
Can be accomplished e.g. using steam, heating, chemicals, or radiation.
We use e-beam irradiation.

Features and Benefits-

Best Fit – Highest Possible Accuracy	Perfect fitting and sealing with
Sartorius pipettes secure the highest
possible accuracy and precision	Compatible with Optiload feature in
Sartorius Picus® Nxt, Picus®, Tacta®,
Mline® and Proline® Plus pipettes
enabling ergonomic and light tip
attachment and ejection	Color-coding of tip trays allows
easy matching with a corresponding­color-coded Sartorius pipette	Compatible with most other pipette
makes

Tip Selection Guide per Application
Tip Type

Optifit Tips

PurityStandard

Regular pipetting applications-

Applications where prevention ofcross-contamination is vital
Pipetting liquids with low surface
tension (e.g. detergents, solvents)---

Premium Quality and Purity:
	Strict quality standards, ISO 9001
and ISO 13485, are followed from
R&D to production and delivery
	Manufactured in ISO 8 classified
clean room conditions
	Manufacturing process free of
DNase, RNase, human DNA andendotoxins: Single Trays, Refill Packs
and FlexiBulk® packs certified pureby lot number	Pre-sterilized tips are e-beam­irradiated	All tip packages, including ­individual
racks, are lot numbered for full­traceability
	The highest quality virgin poly­
propylene used as raw material

Safetyspace™
Filter Tips
Free of DNase, RNase,human DNA & endotoxins-

Pre-sterilized & free of
DNase, RNase, human
DNA & endotoxins-

Low Retention Tips

Pre-sterilized & free of
DNase, RNase, human
DNA & endotoxins

-

-

56

Pipette Tips

Optifit Tips

Optifit Tips

Standard Tips for Various Needs
Sartorius Optifit tips are an excellent
choice for various laboratories andpipetting tasks with their wide rangeof packaging and purity options. The
Optifit tips are packed in single tray
racks, refill towers, single refill packs,
and bulk packages. Optifit tips areavailable DNase, RNase, human DNA
and endotoxin free, as well as e-beam
pre-sterilized.

The Single Tray tip racks are ideal foreasy tip loading and for contamination-free pipetting. In order to reuse
the empty tip racks and to create lesswaste, you may fill the empty racks
with Refill tips, either using the refill
tower or purity certified refill pack tips.
The FlexiBulk® pack is the choice, if
you need a cost-effective, yet purity-­certified solution, in bulk tip format.

Available Packaging Options-----

Single Tray Rack
Refill Tower
Refill Pack
FlexiBulk® Pack
Bulk in a Bag

Features and Benefits	Standard non-filter tips made to
Sartorius quality standards
	Low Retention Tip range alsoavailable for liquids with low surface
tension	Perfect fitting and sealing with
Sartorius Picus® Nxt, Picus®, Tacta®,
Mline®, Proline® Plus, and Proline®
pipettes	Wide tip volume range from 10 µL
to 10 mL

Optifit tips provide excellent tip
sealing----	Wide selection of packaging andpurity options
	Available as DNase, RNase, human
DNA and endotoxin-free	e-beam pre-sterilized packaging
options available
	Full traceability	Color-coded trays to match withcorresponding Sartorius pipettes
	Fully autoclavable at 121 °C, at 1 bar,for 20 minutes

Pipette Tips

Safetyspace™ Filter Tips

57

Safetyspace™ Filter Tips
Protect Valuable Samples

Safetyair gap

Safetyspace™ Filter Tips offer ­optimal
protection for your work from crosscontamination and enable you to
use the full volume of the tip withany p
­ ipetting mode. They feature
the unique Safetyspace™ air gap thatleaves additional space between thesample and the filter that conventional
filter tips do not have. This extra space
prevents the liquid from touching,and permeating, the filter and thus
­guarantees the pipetting accuracy.

---

The Safetyspace™ Filter Tips are
Ideal for:molecular biology
microbiology
cell culture applications
radioactive work

Safetyspace™ Filter tips are made ofvirgin polypropylene and are fittedwith pure polyethylene filter barriers
that effectively capture solid andliquid aerosol particles. The filter ismade without self-sealing additives toavoid any interference with the sample
or results. In addition to ­protecting
your samples, the filter protects
your pipette and prolongs its the­maintenance interval.

---

The Extra Space is Particularly Usefulin the Following Applications:	pipetting foaming liquids such as
buffers and proteins	pipetting solvents	multiple dispensing functions ofelectronic pipettes
reverse pipetting

Available Packaging Options--

Single Tray Rack

Features and Benefits	Filter minimizes the risk of aerosol
contamination	Safetyspace™ air gap between
the sample and filter reduces therisk of contaminating the internal­components of our pipettes	Enables the full use of pipettes’­volume range with any pipettingmode	Available in volumes from 10 µL to
5,000 µL---	Certified free of DNase, RNase,human DNA and endotoxins	e-beam pre-sterilized	Full traceability	Color-coded trays indicate thematching color-coded Sartorius
pipette

58

Pipette Tips

Low Retention Tips

Low Retention Tips

Ensure Optimal Sample Recovery

The four tips on the right are Low
Retention Tips, providing maximum sample recovery

Low Retention Tips maximize yoursample recovery, when handling­detergent con­taining or other liquids
with low surface tension. Pipetting
liquids that contain detergents canbe problematic when using standard
pipette tips. Some liquid residue often
remains in the tip due to ­differencesin surface energies between the­plastic pipette tip and the sample.
The residue causes imprecision in­pipetting and loss of valuable samples
or reagents.

Better reproducibility in pipetting is
especially beneficial in sensitive
molecular biology applications, where
reagents often contain detergents, forexample in:	PCR, real-time PCR	Cloning, sequencing and other DNA& RNA techniques	SDS-PAGE and other protein­analysis methods
	Protein purification techniques---

We use an advanced technology to
manufacture Low Retention Tips thathave an extremely even and durablehydrophobic surface. Unlike someother hydrophobic tips on the market,
our Low Retention Tips do not containany leachables that might risk yoursample.

Available Packaging Options-----

Single Tray Rack
Refill Tower

Features and Benefits
Packaging options for
Low Retention Tips

	Extremely hydrophobic tips surface	Maximum sample recovery for fluidswith low surface tension	Durable, high chemical resistance,
no leachables
	Covers tip volumes from 10 µL to
1,200 µL	Available as Safetyspace™ Filter Tips
and Optifit Tips
	DNase, RNase and endotoxin-freepackaging options available--	e-beam pre-sterilized packaging
options available
	Full traceability	Color-coded trays indicate thematching color-coded Sartorius
pipette	Non-filter tips are fully autoclavable
at 121 °C, at 1 bar, for 20 minutes

60

Pipette Tips

Packaging Options

Packaging Options

Single Tray Racks

--

---

----

--

Racked Tips
Single Tray Rack
	96 tips in convenient and reusable
tray racks (sales unit contains 10 tray
racks, total 960 tips)
	Certified free of DNase, RNase,human DNA and endotoxins	e-beam pre-sterilized option­available
	Lot-specific purity certificates canbe downloaded fromwww.sartorius.com
	Informative rack labelling: ­volume,
product number, lot number­improves tip identification andtraceability	Air-tight plastic wrapping around therack secures purity during transportand storage (wrapping is regularwaste)

Bulk Tips
FlexiBulk®
	Tips made to the Sartorius quality
standard in economical packaging
	Packed orderly in compact re-­sealable plastic packages (480 or
960 pcs depending on tip volume)	Covers a large range of tip volumes
from 200 µL to 1,200 µL
FlexiBulk®

Bulk in a Bag
	Tips made to the Sartorius quality
standard in economical packaging
	Packed in re-sealable bags in cardboard boxes (100, 250 or 1,000 pcs
depending on tip volume)	Covers tip volumes 10 µL, 5 mL and
10 mL
	Tips are fully autoclavable at 121°C, at
1 bar, for 20 minutes	100 % recyclable tips and package

Bulk in a Bag

	Tip trays are color-coded to indicate
the matching, color-coded Sartorius
pipette	Covers a large range of tip volumes
from 10 µL to 10 mL	Fully autoclavable at 121 °C, at 1 bar,for 20 minutes
	Tray racks can be easily reloadedwith Refill tips
	Racks, trays and tips are 100 %­recyclable polypropylene (PP)	Lot-specific purity certificates canbe downloaded fromwww.sartorius.com
	Tips are fully autoclavable at 121 °C,
at 1 bar, for 20 minutes	100 % recyclable tips (PP) and­package (PET)Pipette Tips

Packaging Options

--

--

----

--

Refill Tips
Refill Tower
	Space-saving with 10 × 96 tips in one
tower	Tip trays are compatible with Single
Tray racks for convenient use	Trays are color-coded to indicate
the matching, color-coded Sartorius
pipetteRefill Towers

Single Refill Packs

61

Single Refill Packs
	Individually packed air-tight tip
trays for maximum purity with less­packaging material compared to
racked tips
	10, 15 or 20 trays of 96 tip trays,­depending on tip volume
	Certified free of DNase, RNase andendotoxins	e-beam pre-sterilized option­available
	Lot-specific purity certificates canbe downloaded fromwww.sartorius.com
	Tip trays are compatible with Single
Tray racks for convenient use	Informative rack labelling: ­volume,
product number, lot number­improves tip identification andtraceability

	Covers the most widely used tip
sizes: 10 µL, 200 µL and 350 µL
	Trays and tips are fully autoclavable
at 121 °C, at 1 bar, for 20 minutes	100 % recyclable cardboard­packaging, and plastic (PP) trays
and tips	Tip trays are colour-coded to­indicate the matching, colourcoded Sartorius pipette	Covers a large range of tip volumes
from 10 µL to 1,200 µL
	Trays and tips are fully autoclavable
at 121 °C, at 1 bar, for 20 minutes	100 % recyclable trays and tips (PP).
Container lid is regular waste.

62

Pipette Tips

Pipette Tips

Ordering Information

Optifit Tips
Volume Range

LengthPackaging

0.1 – 10 µL

31.5 mm

Single Tray
Single Tray
Single Tray
Refill Tower
Refill Tower
Refill Pack
Bulk in Bag

0.5 – 200 µL

51 mm

Single Tray
Single Tray
Single Tray
Refill Tower
Refill Tower
Refill Pack
FlexiBulk®

5 – 350 µL

54 mm

Single Tray
Single Tray
Single Tray
Refill Tower
Refill Tower
Refill Pack
FlexiBulk®

10 – 1,000 µL

71.5 mm

Single Tray
Single Tray
Single Tray
Refill Pack
Refill Pack
FlexiBulk®

For your guidance the tips are shown here in the actual size.

Low
Retention-

Purity Level
Free of DNase,
RNase, human
DNA & endotoxins---------

Tips/Unit

Order Code

10 × 96
10 × 96
10 × 96
10 × 96
10 × 96
20 × 96
1,000

790010
LH-L790010
790011790012
LH-L790012
790013790014

10 × 96
10 × 96
10 × 96
10 × 96
10 × 96
15 × 96960

790200
LH-L790200
790201790202
LH-L790202
790203
LH-B790204

10 × 96
10 × 96
10 × 96
10 × 96
10 × 96
15 × 96960

790350
LH-L790350
790351790352
LH-L790352
790353
LH-B790354

10 × 96
10 × 96
10 × 96
10 × 96
10 × 96480

791 000
LH-L791000
791001791002791003
LH-B791004

Pre-sterilized-Pipette Tips

Volume Range

LengthPackaging

Pipette Tips

Low
Retention

Purity Level
Free of DNase,
RNase, human
DNA & endotoxins 10 – 1,000 µL
Wide bore tip

68.5 mm

Single Tray
Single Tray
FlexiBulk®

50 – 1,200 µL

71.5 mm

Single Tray
Single Tray
Single Tray
Refill Pack
Refill Pack
FlexiBulk®

100 – 5,000 µL

150 mmSingle Tray
Single Tray
Bulk in Bag
Bulk in Bag

100 – 10,000 µL

155 mmSingle Tray
Bulk in Bag-

Note: The ordering information for 10,000 µL tip for Midi Plus can be found on page 69.

63------

Tips/Unit

Order Code-

10 × 96
10 × 96480

791020791021
LH-B791024-

10 × 96
10 × 96
10 × 96
10 × 96
10 × 96480

791 200
LH-L791200
791201791202791203
LH-B791204

50
50
100
1,000

78030478030578030078030835
250

LH-780314
LH-780316

Pre-sterilized-

64

Pipette Tips

Pipette Tips

Ordering Information

Safetyspace™ Filter Tips
Volume Range

LengthPackaging

0.1 – 10 µL

31.5 mm

Single Tray
Single Tray

0.5 – 20 µL

51 mm

Single Tray
Single Tray2 – 120 µL

51 mm

Single Tray
Single Tray

0.5 – 200 µL

52.5 mm

Single Tray
Single Tray

0.5 – 300 µL

52.5 mm

Single Tray
Single Tray

50 – 1,000 µL

78 mm

Single Tray
Single Tray

50 – 1,200 µL

90 mm

Single Tray
Single Tray

100 – 5,000 µL

150 mmSingle Tray

Low
Retention-

For your guidance the tips are shown here in the actual size.
Filter tips are not recommended to be used simultaneously with Safe-Cone Filters.

Purity Level
Free of DNase,
RNase, human
DNA & endotoxins---------

Tips/Unit

Order Code

10 × 96
10 × 96790011F
LH-LF790011

10 × 96
10 × 96790021F
LH-LF790021

10 × 96
10 × 96790101F
LH-LF790101

10 × 96
10 × 96790201F
LH-LF790201

10 × 96
10 × 96790301F
LH-LF790301

10 × 96
10 × 96791001F
LH-LF791001

10 × 96
10 × 96791211F
LH-LF791211

50

LH-795001F

Pre-sterilized---------

Pipette Tips

Pipette Tips

65

Extended Standard Tips
Volume Range

LengthPackaging

Low
Retention

Purity Level
Free of DNase,
RNase, human
DNA & endotoxins

0.1 – 10 µL

46 mm

Single Tray
Single Tray

0.5 – 200 µL

77.5 mm

Single Tray
Single Tray

10 – 1,000 µL

102 mmSingle Tray
Single Tray

50 – 1,200 µL

90 mm

Single Tray
Single Tray
Single Tray
Refill Pack
Refill Pack

0.1 – 10 µL

46 mm

Single Tray

0.5 – 200 µL

77.5 mm

Single Tray

10 – 1,000 µL

102 mmSingle Tray-

Extended Filter Tips

For your guidance the tips are shown here in the actual size.
Extended filter tips are not recommended to be used simultaneously with Safe-Cone Filters.
The liquid handling properties of extended tips might differ from standard Optifit tips.

Tips/Unit

Order Code

10 × 96
10 × 96783210783211

10 × 96
10 × 96

LH-X780200
LH-X780201

8 × 968 × 96

LH-X781000
LH-X781001

10 × 96
10 × 96
10 × 96
10 × 96
10 × 96791210
LH-L791210
791211791212791213

10 × 96783201

10 × 96

LH-XF780201

8 × 96

LH-XF781001

Pre-sterilized------

-

-

-

-

67

Maxi-volume Liquid Handling
Table of Contents

Maxi-volume Liquid Handling
Midi Plus Pipetting Controller
Prospenser Plus and Prospenser
Biotrate Digital Burettes

68
70
72

68

Maxi-volume Liquid Handling

Midi Plus

Midi Plus Pipetting Controller
Excellent Performance and Ergonomics
The Midi Plus is a lightweight electronic cordless pipetting controller,
which allows aspiration from bottles
and tubes, without the arm and handelevations required in the case of serological or volumetric pipettes.
It fits all commonly used 1 – 100 mL
glass or plastic pipettes, but can also
be used with Sartorius 5 mL and 10 mL
disposable tips. The speed can be
fine-tuned by applying varying finger
pressure to the operating buttons.

The Midi Plus is ideal, for example, in
microbiological work: dispensing intoa culture media dish can be performed
carefully, drop by drop, without breaking the fine surface of the medium.

--	Stepless speed control
	Hydrophobic autoclavable filter protects the device in case of over-aspiration
	Convenient fold-out bench standsupports the unit and pipette when
not in use
	Low battery warning

Features
Pipette types

Plastic or glass 1 – 100 mL
Pasteur pipettes
5 mL and 10 mL Sartorius pipette tips

Rechargeable during use

Yes

Speed control

Stepless adustable control

Gravity dispensing

Yes

Stand

Attached support

Weight207 g

Low battery indicator

Yes

Autoclavable parts

Nose cone, pipette holder and filter

Maxi-volume Liquid Handling

Midi Plus

69

Ordering Information
Order CodeItem

Qty / Unit

710931

Midi Plus Pipetting Controller with Universal Charger1

1

LH-7129120

Replacement filter, 0.45 µm, non-sterile, autoclavable5

LH-7129131

Replacement filter, 0.2 µm, sterile5

LH-711019

Adapter set (standard), autoclavable, including nose-cone 1
and silicone adapter

LH-711017

Adapter set for 5 mL pipette tip, autoclavable, including
nose-cone and silicone adapter

1

LH-711018

Adapter set for 10 mL pipette tip, autoclavable, including
nose-cone and silicone adapter

1780300

Optifit Tip 5 mL (length 150 mm)

100780308

Optifit Tip 5 mL (length 150 mm)

1,000

780310Midi Plus Tip 10 mL (length 242 mm)

100

1 Supplied with a universal charger (incl. EU, UK, US | JPN, AUS, KOR and CHN plugs)

70Maxi-volume Liquid Handling

Prospenser Plus and Prospenser

Prospenser Plus and Prospenser
Dispensing Made Easy

Chemical Resistance
Prospenser and Prospenser Plus offer
excellent chemical resistance thanks
to their high quality materials. The
ceramic piston is chemically stable andcompatible with an extremely wide
range of liquids.
Light to Use
The operating handle is designed tobe comfortable to hold while aspirating and light to press while dispensing.
The piston moves smoothly, guaranteeing excellent results in everyday use.
Easily Replaceable Valves
Valves can be easily replaced by theuser so there is no need to send theunit to be serviced.
Easy to Attach Aspiration Tube
The aspiration tube is easy to attachwith a secure threaded connection.
This prevents air entering the systemand ensures consistent results.
Volume Setting
Prospenser and Prospenser Plus
have an incremental volume setting
enabling you to set the exact volume
every time.

Versatile Accessories
There is a wide range of accessories
available including bottle adapters,
dispensing heads, and aspiration
tubes.
Fully Autoclavable
Prospenser and Prospenser Plus areeasy to disassemble for cleaning andare fully autoclavable.

Maxi-volume Liquid Handling

Prospenser Plus and Prospenser

Prospenser Plus – Advanced Features
Media Recirculation System
Prospenser Plus is equipped witha media recirculation system thatallows unused media to be recoveredfrom the device and returned to thebottle. This ensures easy priming and­minimizes media loss.
Smart 360° Rotating
Dispensing Head
The dispensing head can be turned to
the required position without needing
to turn the bottle. This ensures thebottle label is always visible to the user.
Connector for Drying Tubeand Filters
Prospenser Plus has a luer connection
at the back of the unit for drying tubes
and filters. Drying tubes are recommended for humidity sensitive liquids.

71

72

Maxi-volume Liquid Handling

Biotrate

Biotrate Digital Burettefor Convenient Titration

Sartorius Biotrate is a premium digital
burette equipped with sophisticatedfunctions for easy titration. Biotrateoffers high chemical resistance and it
is a smart solution for titrating various
liquids accurately and safely. Biotrateis easy and convenient to use due to
its large and clear electronic display,smooth operating wheels, and 360°
rotating dispensing head.

-High Chemical Resistance	Highly resistant parts in the ­liquid
pathway guarantee excellent­chemical resistance.
	The softly-moving piston ensuresreliable results.
Premium Quality
	High quality parts and materials
guarantee highly accurate and precise results as well as long lifetime.
Convenient to Use	Biotrate is comfortable to use due
to soft touch operating wheels and360° rotating dispensing head.
	Extremely smooth and light tooperate. Ordering Informationand ­Performance Specifications­Chemical Resistance	Biotrate offers excellent chemical
resistance due to its high-quality
materials, allowing the use of wide
variety of liquids.

-

Light to Use	The operating wheels are light andcomfortable to use due to the soft
touch surface and smoothly ­moving
piston. These guarantee goodergonomics and excellent results ineveryday use.

-

Long Life Battery	Biotrate is powered with a long life
battery so there is no need to plug inor re-charge the unit. The battery is
also easy to replace by the user.
Large and Clear Display
	The display indicates clearly themeasured volume, and showswhether you are measuring an­aspirated or titrated volume.
Easy to Clean	Biotrate is easy to disassemble forcleaning and autoclaving. The liquid
pathway is fully autoclavable.
User Adjustment
	Biotrate can be adjusted for ­various
liquids and it is easy to revert tofactory settings. The display also
indicates when a custom adjustment
is in use.

Maxi-volume Liquid Handling

Prospenser Plus, Prospenser and Biotrate

73

Ordering Information
Prospenser Plus
Order Code

Item

Volume Range

Increment

Systematic
Error (%)

Systematic
Error (mL)

RandomError (%)

RandomError (mL)

LH-723070

Prospenser Plus

0.2 – 1 mL

0.05 mL

0.6

0.006

0.2

0.002

LH-723071

Prospenser Plus

0.4 – 2 mL

0.05 mL

0.6

0.012

0.2

0.004

LH-723072

Prospenser Plus

1 – 5 mL

0.10 mL

0.6

0.03

0.2

0.01

LH-723073

Prospenser Plus

2 – 10 mL

0.25 mL

0.6

0.06

0.2

0.02

LH-723074

Prospenser Plus

5 – 30 mL

0.50 mL

0.6

0.18

0.2

0.06

LH-723075

Prospenser Plus

10 – 60 mL

1.00 mL

0.6

0.36

0.2

0.02

Note: All Prospenser Plus models have A45 thread as a default and they are supplied with A32, A38, and S40 bottle adapters. Bottle not included

Prospenser
Order Code

Item

Volume Range

Increment

Systematic
Error (%)

Systematic
Error (mL)

RandomError (%)

RandomError (mL)

LH-723060

Prospenser

0.2 – 1 mL

0.05 mL

0.6

0.006

0.2

0.002

LH-723061

Prospenser

0.4 – 2 mL

0.05 mL

0.6

0.012

0.2

0.004

LH-723062

Prospenser

1 – 5 mL

0.10 mL

0.6

0.03

0.2

0.01

LH-723063

Prospenser

2 – 10 mL

0.25 mL

0.6

0.06

0.2

0.02

LH-723064

Prospenser

5 – 30 mL

0.50 mL

0.6

0.18

0.2

0.06

LH-723065

Prospenser

10 – 60 mL

1.00 mL

0.6

0.36

0.2

0.02

Note: All Prospenser models have A32 thread as a default and they are supplied with A28, S40, and A45 bottle adapters. Bottle not included

Biotrate Digital Burette
Order Code

Item

Volume Range

Increment

Systematic
Error (%)

Systematic
Error (mL)

RandomError (%)

RandomError (mL)

LH-723080

Biotrate

10 mL

0.01 mL

0.3

0.03

0.1

0.01

LH-723081

Biotrate

20 mL

0.01 mL

0.2

0.04

0.1

0.02

LH-723082

Biotrate

50 mL

0.01 mL

0.2

0.10

0.1

0.05

Note: All Biotrate models have A45 thread as a default and they are supplied with A32, A38, and 40 bottle adapters. Bottle not included

74

Maxi-volume Liquid Handling

Prospenser Plus, Prospenser and Biotrate

Accessories
Biotrate

UV-Protection Window
Biotrate is supplied with an ambercolored replacement window forprotecting light sensitive media.

Bottle support, for bottles
75 – 120 mm

Aspiration tube, 310 mm

Dispensing head, 1.5 mm spiral

Order Code

Item

Material

LH-721650

Dispensing head, standard

FEP

LH-721651

Dispensing head, fine tip

FEP

LH-721648

Dispensing head, 1.5 mm spiral

FEP

LH-721652

Dispensing head, 0.4 m adjustable

FEP

LH-721653

Dispensing head, luer lock

FEP | PP

LH-721654

Dispensing head, luer lock

FEP | PFA

LH-721678

Aspiration tube, 310 mm

FEP

LH-721679

Aspiration tube, 400 mm

FEP

LH-721682

Recirculation tube, 70 mm

FEP

LH-721689

Bottle adapter, A28

ETFE

LH-721684

Bottle adapter, A28

PP

LH-721683

Bottle adapter, A32

PP

LH-721688

Bottle adapter, A32

ETFE

LH-721686

Bottle adapter, A38

PP

LH-721733

Bottle adapter, A38

ETFE

LH-721685

Bottle adapter, A38 | 430

PP

LH-721732

Bottle adapter, A38 | 430

ETFE

LH-721687

Bottle adapter, S40

PP

LH-721734

Bottle adapter, S40

ETFE

LH-721735

Bottle adapter, NS29 | 32

Silicone

LH-721743

Bottle support, for bottles 75 – 120 mm

Silicone

LH-721744

UV-Protection Window------------

Biotrate

Maxi-volume Liquid Handling

Prospenser Plus, Prospenser and Biotrate75

Prospenser Plus
Order Code

Item

Material

LH-721647

Dispensing head, standard

FEP

LH-721655

Dispensing head, standard

FEP

LH-721656

Dispensing head, standard

FEP

LH-721648

Dispensing head, 1.5 mm spiral

FEP

LH-721649

Dispensing head, 3.0 mm spiral

FEP

LH-721657

Dispensing head, spiral

FEP

LH-721658

Dispensing head, spiral

FEP

LH-721653

Dispensing head, luer lock

FEP | PP

LH-721654

Dispensing head, luer lock

FEP | PFA

LH-721659

Dispensing head, luer lock

FEP | PP

LH-721660

Dispensing head, luer lock

FEP | PFA

LH-721678

Aspiration tube, 310 mm

FEP

LH-721680

Aspiration tube, 310 mm

FEP

LH-721679

Aspiration tube, 400 mm

FEP

LH-721681

Aspiration tube, 400 mm

FEP

LH-721682

Recirculation tube, 70 mm

FEP

LH-721736

Bottle adapter, A25

PP

LH-721737

Bottle adapter, A28

PP

LH-721689

Bottle adapter, A28

ETFE

LH-721684

Bottle adapter, A28

PP

LH-721683

Bottle adapter, A32

PP

LH-721688

Bottle adapter, A32

ETFE

LH-721686

Bottle adapter, A38

PP

LH-721733

Bottle adapter, A38

ETFE

LH-721738

Bottle adapter, A38

PP

LH-721685

Bottle adapter, A38 | 430

PP

LH-721732

Bottle adapter, A38 | 430

ETFE

LH-721741

Bottle adapter, A45

ETFE

LH-721739

Bottle adapter, A45

PP

LH-721687

Bottle adapter, S40

PP

LH-721734

Bottle adapter, S40

ETFE

LH-721740

Bottle adapter, S40

PP

LH-721742

Bottle adapter, S40

ETFE

LH-721735

Bottle adapter, NS29 | 32

Silicone

LH-721743

Bottle support, for bottles 75 – 120 mm

Silicone

Prospenser Plus

Prospenser

Prospenser

All models

1, 2, 5 & 10 mL

30 & 60 mL---------

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76

77

Pipetting Academy
Table of Contents

Pipetting Academy
Pipetting Academy
Pipetting Recommendations

78
80

78

Pipetting Academy Pipetting Academy

Pipetting Academy

Training for Better Performance, Ergonomics and Safety
Sartorius Pipetting Academy offers­insight and training for pipetting­related topics. It’s a ­comprehensive
combination of theory and ­practicethat provides you with useful­knowledge and tips for your daily work.
Thousands of laboratory professionals
around the globe have been trainedat the Pipetting Academy over the lasttwo decades.

Pipetting Academy­Training Modules
Basics of Pipetting Module
Basics module brings together the
essentials of pipetting techniques,
ergonomics, and calibration. It is an­excellent package for anyone who is
new to pipetting or feels a need topolish pipetting practices. In basic
module you will learn:
to choose the right equipment
the basics of pipetting techniques
the best practices in lab ergonomics
daily maintenance and checking of
pipettes---			-	

Ergonomics Module
The ergonomics module is i­ntended
for laboratory personnel and­occupational and health p­ rofessionals
who are interested in improving
the work ergonomics, minimizing­ergonomic risk factors and ­preventing
musculoskeletal disorders. In the­ergonomics module you will learn:	to recognize the ergonomic risk
factors in pipetting and lab work	to select the right tools and working
postures	to apply the best ergonomics andmake your work more enjoyable--

Pipetting Techniques Module
Pipetting is a precision task influenced
by multiple factors such as equipment,
pipetting techniques, liquids, andenvironmental conditions. In pipetting
techniques module you will learn:
to understand the influence of
pipetting techniques on pipetting
results
to identify the influence of different
error sources on pipetting results
how to select pipetting tools andtechniques for different liquids andconditions-	--		

Pipetting in Cell Culture
Applications Module
Working in cell culture lab has special
requirements in terms of techniques
and the purity of equipment. In the cell
culture module you will learn:	how to avoid contamination
	the best pipetting practices for cell
culture work	to recognize the factors affecting
your results--

Pipetting Standards and­Maintenance Module
Regular pipette maintenance andcalibration ensures reliable results
and conformance to regulation. In
Pipetting Standards and Maintenance
Module you will learn:	to define your pipette maintenanceprogram according to ISO 8655
standard	to handle pipettes as precision
instruments	to ensure reliable performance ofyour pipettes--

Pipetting Academy Pipetting Academy

Best Practices for Working
with Proteins
To achieve the best results, ­protein
samples should have a high­concentration and maintain a highbiological activity. In this Pipetting
Academy Module you will learn:	best pipetting practices for protein
work	how to ensure your sample quality
and yield--

Best Pipetting Practices for
Nucleic Acid WorkDNA and RNA extraction and qPCR
assays are the cornerstones of many
research in life science. In Best
­Pipetting Practices for Nucleic Acid
Work you will learn	to maintain sample quality and purity	to avoid contamination	to recognize the factors affecting
your results---

Best Practices for Cell-based
Sample Preparation
Have you ever wondered if sample
preparation could be the source ofvariance you see in your cell-based­assays? In Best practices for Cellbased Sample Preparation Pipetting
Academy you will learn:	the aspects that affect your cellbased assays with Incucyte or IQue
screener	how to reduce variability between
experiments	how to speed up the workflow andimprove your cell health with smart
pipetting practices--

79

Contact Sartorius to
Set Up Your Tailored­Pipetting Academy---	To sign up for the seminar, contact
your local Sartorius representative	The seminar will be held in the­location most suitable to you andyour colleagues
	The trainer will be certified to hold
Pipetting Academy seminars
	Each participate will receive a
certificate of participation after theseminarSee You at the Pipetting Academy!

80

Pipetting Academy Pipetting Recommendations

Pipetting Recommendations

Hold the pipette in a vertical position during aspiration.

Avoid contamination with SafeCone Filters.

Tacta® volue lock can be usedin two ways, by pressing it whilechanging the volume, or by sliding
it up to open it, and back to lock it.

----

Preparations Before Pipetting	Use the tip specified by the­manufacturer.
	Ensure that the pipette and thetip have been tested according
to ISO 8655 and the tip is seated­correctly.
	Make sure pipettes have been­correctly calibrated.
	Check that the pipette, tip and liquid
are all at the same temperature.
	When pipetting liquids with­temperatures different to the­ambient temperature, do not prerinse the tip. Change the tip after
each pipetting.
	Ensure that any fluid viscosity­variations have been accounted
for and the correct technique is­employed, i.e. reverse pipetting.
	If handling infectious or ­radioactive
agents make sure appropriate
shielding and other precautions
protect the operator.
	Use Safe-Cone Filter in the tip cone
whenever possible.
	Use racked tips when possible forbest ergonomics--

While Pipetting	Hold the pipette in a vertical­position, while aspirating. Tilting thepipette at an angle causes a volume
greater than the set volume of liquid
to enter the tip.
	In most cases, pre-rinsing of the
tip is recommended, to achieve­accurate results. Do not pre-rinse
the tip, if the temperature of theliquid is ­different to the ambient­temperature.
	When aspirating fluid, the pipette tip
should normally be immersed to adepth of 2 – 3 mm.
	When using a mechanical pipette,
operate the piston with a smoothand consistent thumb action, forrepeated results without foaming or
bubbles.
	You should pipette against the insidewall of the receiving vessel. Remove
the tip by drawing it upwards against
the inside wall.
	Ensure that the pipette blow out
action is fully activated.
	Ensure that the volume is still set
at the required position. A pipettewith a volume locking mechanism
is recommended, in order to avoid
accidental volume change during
pipetting.
	Avoid laying the pipette on its side
while there is liquid in the tip. It may
seep up into the mechanism.

Pipetting Academy Pipetting Recommendations

Charging it while pipetting ispossible with Sartorius electronic
pipettes.

Load the tip onto the pipette carefully and take advantage of the
Optiload tip loading mechanism.

Clean the pipette before sending
it to service.

---

Other Precautions
	Store the pipette on a stand when
not in use – see page 46, on ­pipettestands, for more ­information.
­Electronic pipettes should be­returned to their charging stands.
	Avoid dropping the pipette or­allowing contact with dirt or grease.
	Change the Safe-Cone Filter­regularly (recommendation after 50
to 250 pipetting cycles), and in every
case of over-aspiration.
	Never strike the tip cone against the
tip tray when loading the tip, as thiscan damage the pipette.
	Avoid exposing the unit to extreme
temperature changes, humidity anddust (operating temperature from
15 °C to 40 °C).
	Service the pipette regularly.
	Clean the pipette thoroughly before
sending it in for service.
­Decontaminate the pipette with
70 % ethanol. Notify the service­personnel of the purpose for whichthe ­instrument has been used.
Postal services may refuse to deliver­instruments used for hazardous
materials. Make sure that a qualified
person services the pipette.

81

83

Calibration and Maintenance
Table of Contents

Pipette Calibration and Maintenance
Pipette Calibration and Maintenance Services
Pipette Decontamination Procedure
Autoclaving Instructions

84
85
86

84

Calibration & Maintenance

Pipette Calibration and Maintenance Services

Pipette Calibration and Maintenance Services
Why is Calibration and
Maintenance Needed?
Pipette calibration is a fundamental
part of Good Laboratory Practice(GLP) and quality systems and must
be considered a vital part of any­laboratory regime where precise
volumes of fluid need transferringor diluting. Pipette performance is­measured as accuracy and precisionor how close the dispensed volumes
are to the target and how close theresults are to each other.
Adopting a regular calibration andmaintenance routine for your pipettehas the following benefits:
ConfidenceYour pipettes are operating correctlywith the accuracy and precision you
know.
Reliability
With maintenance and calibration andoperational qualification you are able
to trust the instrument’s operational
capability.
Efficiency and EffectivenessWith properly working pipettes you
can work uninterrupted and be more
efficient.

Sartorius Service Centres
It is vitally important to Sartorius that
our customers receive world class­service and support, from the firstphone call to the moment the­engineer leaves, with the ­customer’sequipment in perfect working­condition.
Sartorius provides a global networkof service centers for calibration of
all makes and models of pipettes,­burettes, bottle top dispensers andother liquid handling instruments.
With over 20 years of experiencein ­pipettes and liquid handling­instrument services, the globalorganization provides world classservices tailored to customers’ needs,
simultaneously fulfilling standards andregulatory body requirements. With
the Sartorius concept of “all makes andmodels liquid handling services” you
can be certain that your instruments
are handled according to the international ISO 8655 standard defining
the use and calibration of pipettes
and other liquid handling ­instruments.
­Sartorius provides reliability of­operation, reduced instrument
downtime and confidence, so thatyour work is according to the strictest­requirements.

Calibration & Maintenance

Pipette Calibration and Maintenance Services

85

Pipette Decontamination Procedure

Note: When opening pipette lower parts, as a matter of routine
the parts should be inspected for possible wear. Always checkthe pipette performance after opening the lower parts.

Mechanical Pipettes(Tacta®, Mline® and Proline® Plus)

Electronic Pipettes(Picus®, Picus® Nxt)

1.	 Unscrew the tip ejector collar counter­clockwise and remove it. Remove the­Safe-Cone Filter if fitted.

1.	 Unscrew the tip ejector collar counter­clockwise and remove it. Remove the­Safe-Cone Filter if fitted.

2.	 Unscrew the tip cone holder counter­clockwise and carefully remove it along withthe tip cone.

2.	 Unscrew the tip cone holder counterclockwise and carefully remove the tip cone
holder, tip cone and spring.

3.	 The piston is left on the mechanical pipette.
4.	 Place the tip ejector collar, tip cone holder,tip cone and tip cone cylinder into a beakercontaining 70 % ethanol and leave for at least
30 minutes.

3.	 Unscrew the piston counter clockwise fromthe pipette.
4.	 Place the tip cone, tip cone holder, tip
ejector collar, piston and spring into a beaker­containing 70 % ethanol and leave for at least
30 minutes.

5.	 After performing the procedure describedabove, remove the components from thebeaker and rinse them with distilled water,then dry preferably with warm air, for at least
an hour.
6.	 Re-grease the piston as described in the
instruction manual. Replace all components
including new filter if fitted.

5.	 After performing the procedure describedabove, remove the components from thebeaker and rinse them with distilled water,then dry preferably in warm air, for at least
an hour.
6.	 Re-grease the piston as described in the
instruction manual. Replace all components
including the new filter if fitted.

86

Calibration & Maintenance Autoclaving Instructions

Autoclaving Instructions
Tacta®, Mline® and Proline® Plus
Mechanical Pipettes
The entire Tacta®, Mline® and ­Proline®
Plus mechanical pipette can be
steam sterilized by autoclaving at
121 °C (252 °F), at 1 bar (15 p.s.i.), for
20 minutes. The dispensing head ofthe multi-channel pipettes must be
unscrewed 360° counter clockwise
before autoclaving.

--	Remove the Safe-Cone Filter(if fitted)
	Place the pipette into the­sterilisation bag and place it into
the autoclave	After autoclaving the pipette must
be cooled down and left to dry­overnight before use

It is recommended that you checkthe performance of the pipette after
every autoclaving, and grease thepiston | seal of the pipette after every
10th autoclaving.
Picus® and Picus® Nxt Electronic
Pipettes Lower Parts
The dispensing head (tip ejector
collar, tip cone holder, tip cone, springand piston) of the single-channel
and multi-channel models (except
for multi-channel 1,200 µL) canbe ­autoclaved (121 °C, at 1 bar, for20 ­minutes). These parts can be­autoclaved as one unit or separately
as insdividual parts. It is also possible
to clean the parts and grease the­piston prior to autoclaving.

--	Remove the Safe-Cone Filter(if fitted).
	Put the dispensing head into thesterilisation bag and place it into the
autoclave	After autoclaving the parts must
be cooled down and left to dry
before use

It is recommended that you checkthe performance of the pipette after
every autoclaving, and grease thepiston | seal of the pipette after every
10th autoclaving.

--Tips and Tip Boxes	Place the bulk tips into the­sterilisation bag and the tip tray
as such in the autoclave	Autoclave, at 1 bar, for 20 minutes
at a temperature of 121 °C
	Cool before use
Note:	Excessive heat or length of time
may damage the products. Neverplace the handle part of the Picus®
or Picus® Nxt into the autoclave	The lower ends of multi-channel
pipettes are not interchangeable
between 8 and 12-channel pipettes
	The cover of the tip tray should beclosed during autoclaving

Germany
Sartorius Lab Instruments GmbH & Co. KG
Otto-Brenner-Strasse 20
37079 Goettingen
Phone +49 551 308 0

Finland & Baltics
Sartorius Biohit Liquid Handling Oy
Laippatie 100880 Helsinki
Phone +358 9 755 951

For further information, visit
www.sartorius.com

Specifications subject to change without notice.
Copyright Sartorius Lab Instruments GmbH & Co. KG.
Version 11 | 2020

USA
Sartorius Corporation
565 Johnson Avenue
Bohemia, NY 11716
Phone +1 631 254 4249
Toll-free +1 800 635 2906