RWDLifeScience

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Animal Administration and Blood Sampling Solution

Ordering Instructions:

SkullInjector

DentalCement Screws

GuideCannula

Drug Delivery Cannula
Cannula implantation is the international general method for acute and chronic intracranial drug administration in animal experiments.
RWD offers customized services for users, the users can customize according to specific experimental requirements.

CG1/G2

TargetNuclei

Single Cannula

DiagramforCustomization

D-outer diameter of the Guide Cannula（Unit:g ）, five sizes 22, 23, 24, 26 and 27g are available.

Components:Guide cannula

Dummy cannula
Stainless steel wire and threaded plastic
cap make a dummy cannula. It can beinserted into guide cannula and fixed byscrew to seal the guide cannula andprevent outflow of tissue fluid after
injection. The length of the stainlesssteel wire can be customized.

Guide cannula consists of a threaded
cylindrical plastic pedestal and astainless steel tube. It has to bepreimplanted into brain and fixed withdental cement. Both dummy cannulaand internal cannula can be inserted
into guide cannula individually.

Metal Cannula
The metal materials
avoid biting after thecannula implantation.

Internal cannulaInternal cannula can be
fixed into guide cannula
through fixing screw and its
tail end is connected withPE tube for injections. The
length can be customized.

C-length (Unit: mm) of the metal tube below the plastic pedestal (B).
G1-length (Unit: mm) of the protrudent Injector through the Guide Cannula, 0.5 or 1.0mm is recommended.
G2-length (Unit: mm) of the protrudent Dummy Guide, 0.5 or 1.0mm is recommended.

Order information (Unit: mm):Matching

Fixing screwFixing screw fixes internal

Guide Cannula Internal Cannula Dummy Cannula Dummy Cannula Fixing Screw PE Tube Range of customized length(OD x ID )(OD x ID )(plastic OD)(metal OD)
CG1
G2
62001(23g)
0.64x0.4562201
0.41×0.25

62101
0.4

62106
0.4

62002(24g)0.56x0.3862202
0.36×0.2

62102
0.3

62107
0.3

62003(26g)
0.48x0.3462203
0.3×0.14

62102
0.3

62107
0.3

62004(27g)0.41x0.2562204
0.21×0.11

62104
0.2

62108
0.2

62005(22g)
0.71x0.4562201
0.41×0.25

62101
0.4

62106
0.4

cannula to guide cannula.
CannulaCording Diagram:

62502

62329

0.5~15 0.5~15 0.5~15

Fixing Screw

Internal Cannula

Notes: The height and external diameter of the cylindrical plastic base are 8mm and 3.5mm respectively. Plastic or mental dummy cannula is optional.

Dummy Cannula

Guide Cannula

Double Cannula

Animal Administration andBlood Sampling Solution

Single-Cannula connection

Diagrams:
A - The length of the stainless steel tubing below the plastic pedestal.
B - The height of the cylindrical plastic base.
C - The length of the stainless steel tubing below the plastic pedestal,can be customized.
D - External diameter of stainless steel guide cannulas.
E - External diameter of cylindrical plastic base.
F1 - External diameter of Fixing screw.

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F2 - External diameter of Dummy Cannulas.
H1 - Height of Fixing screw.
H2 - Height of Dummy Cannulas.
G1- The internal cannulas projection length beyond the guidecannulas tip, can be customized.
G2- The dummy cannulas projection length beyond the guidecannulas tip, can be customized.

Animal Administration andBlood Sampling SolutionIntroduction:
RWD has launched I, II, III and IV series double cannula systems for acute and chronic intracranial drug administration in animalexperiments.
Series I: Recommended for rat or mouse use, 0.48mm (outer diameter) x 0.34mm (internal diameter).
Series II: Recommended for rat use, 0.64mm (outer diameter) x 0.45mm (internal diameter).
Series III: Specifically for mouse use, 0.48mm (outer diameter) x 0.34mm (internal diameter). The height of cylindrical plastic pedestal of
Guide cannula is different from Series I.
Series IV: 0.41mm (outer diameter) x 0.25mm (internal diameter).

Application:
Double cannula system is used for bilateral implantation, suitable for injecting one or two drugs into two different brain regions.

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Cording Diagram:

Order Information: (Unit: mm)
Series I: Recommended for rat or mice
Matching

Fixing Screw-Double

Cap-Double

Injector-Double
Dummy Cannula-Double

Cannula （26G）
O.D.0.48mmxI.D.0.34 mm
Dummy Cannula:
O.D.0.30mm
Internal Cannula:
O.D.0.30mm x I.D.0.14mm
Plastic Pedestal of Guide Cannula:
Height B = 7.8mm
Diameter E = 3.5mm

Guide Cannula-Double

Plastic Pedestal of Guide Cannula:
Diameter E = 5 mm

C.C Guide Cannula

CapFixing
Screw PE Tube

62523

62521

Internal Cannula Dummy Cannula

0.5

62021

62250

62121

0.6

62022

62250

62122

0.8

62023

62223

62123

1.0

62024

62224

62124

1.2

62025

62225

62125

1.3

62026

62226

62126

1.4

62027

62227

62127

1.5

62028

62228

62128

1.6

62029

62229

62129

1.8

62030

62230

62130

2.0

62031

62231

62131

2.2

62032

62232

62132

2.4

62033

62233

62133

2.6

62034

62234

62134

2.762035

62235

62135

3.062036

62236

62136

62524

Range of customized length
CG1

G2

0.5~13.7

0,0.5~6

0,0.5~2

62320

62522

62329

CapFixing
Screw

PE Tube

62523

62521

62323

Series II: Recommended for rat

Dual-Cannula connection

Comments:
B - Height of cylindrical plastic pedestal which is 7.8mm for Series I, II & IV and 5.5mm for Series III★C - Length of stainless steel tube below the cylindrical plastic pedestal. The default value is 13.7mm for Series I & III, 20.0mm for Series
II and 10.0mm for Series IV. The length can be customized with default value as maximum

Matching

C.C

Guide Cannula

Cannula(23G)
O.D.0.64mmx I.D.0.45 mm
Dummy Cannula:
O.D.0.40mm
Internal Cannula:
O.D.0.41mm x I.D.0.25mm
Plastic Pedestal of Guide Cannula:
Height B = 7.8mm
Diameter E = 3.5 mm

0.862037

62237

62137

1.062038

62238

62138

1.2

62039

62239

62139

1.362040

62240

62140

1.4

62041

62241

62141

1.562042

62242

62142

1.862043

62243

62143

2.0

62044

62244

62144

2.2

62045

62245

62145

2.4

62046

62246

62146

2.5

62047

62247

62147

2.8

62048

62248

62148

3.0

62049

62249

62149

  ★C.C - Center distance between double guide cannulas, double dummy cannulas and double injectors. These 3 values should be thesame in one system. It can be customized (range: 0.5mm-3.0mm)
D - Outer diameter of stainless steel tube and it has 3 sizes: 0.48mm for Series I & III, 0.64mm for Series II and 0.41mm for Series IV
E - Outer diameter of the cylindrical plastic pedestal and it comes with 3.5mm or 5.0mm. It is related to the outer diameter and centre distance
between double guide cannulas

Plastic Pedestal of Guide Cannula：
Diameter E = 5 mm

F1 - Outer diameter of fixing screw and it comes with 5.0mm (when E=3.5mm) or 7.0mm (when E=5.00mm)
F2 - Outer diameter of guide cannula cap and it comes with 5.5mm (when E=3.5mm) or 7.0mm (when E=5.0mm)

Internal Cannula Dummy Cannula

62524

62522

62329

CapFixingScrewPE Tube62523 62521

62320

Range of customized length
CG1G2

0.5~200,0.5~40,0.5~5

H1 - Height of the fixing screw and it comes with 7.5mm (when E=3.5mm) and 8.0mm (when E=5.0mm)
★G1 - Length of protruding part after internal cannula is inserted into guide cannula. This length can be customized

Series III: Recommended for mice
Matching

C.C

Guide Cannula

0.5

62050

62250

62121

Cannula （26G）
O.D.0.48mmxI.D.0.34 mm
Dummy Cannula:
O.D.0.30mm

0.862052

62223

62123

1.062053

62224

62124

1.262054

62225

62125

Internal Cannula：
O.D.0.30mm x I.D.0.14mm
Plastic Pedestal of Guide Cannula:
Height B = 5.5mm
Diameter E = 3.5mm

1.3

62055

62226

62126

1.4

62056

62227

62127

1.562057

62228

62128

1.6

62058

62229

62129

1.8

62059

62230

62130

2.0

62060

62231

62131

★G2 - Length of protruding part after dummy cannula is inserted into guide cannula. This length can be customized
Animal Administration andBlood Sampling Solution

Ordering Instructions:
Determine the target brain region for drug administration and then determine target values of C, C.C, G1 and G2. These values can notexceed the default ones. Please refer to :★ part of comments of cording diagram for more detail.
★ C: Maximal customized length is 13.7mm for Series I & III, 20.0mm for Series II and 10.0mm for Series IV.
★ C.C: Customized range is 0.5mm-3.0mm.
★ G1: Maximal customized length is 6.0mm for Series I, 4.0mm for Series II, 8.0mm for Series III and 4.0mm for Series IV.
★ G2: Maximal customized length is 2.0mm for Series I, 5.0mm for Series II, 4.0mm for Series III and 5.0mm for Series IV.

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Internal Cannula Dummy Cannula

Range of customized length
C

G1

G2

0.5~13.7

0,0.5~80,0.5~4

Animal Administration andBlood Sampling Solution

H2 - Height of guide cannula cap and it comes with 7.00mm (when E=3.5mm) and 7.5mm (when E=5.0mm)

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Osmotic Pumps

Series IV: Recommended for mice
Matching

Cannula (27G)
O.D.0.41mm x I.D.0.25 mm
Dummy Cannula:
O.D.0.20mm
Internal Cannula:
O.D.0.21mm x I.D.0.11mm
Plastic Pedestal of Guide Cannula:
Height B = 7.8mm
Diameter E = 3.5mm

Internal Cannula Dummy CannulaCap

Range of customized length
FixingScrew PE TubeC
G1G2

C.C

Guide Cannula

0.5

62061

62251

62161

0.6

62062

62251

62162

0.8

62063

62263

62163

1.0

62064

62264

62164

1.2

62065

62265

62165

1.3

62066

62266

62166

1.4

62067

62267

62167

1.5

62068

62268

62168

1.6

62069

62269

62169

1.8

62070

62270

62170

Storage capsules have good drug compatibility, andprotectionforshorthalf-lifedrugs.

2.062071

62271

62171

Capsules have good biocompatibility and do not affect the physiology of animals.
Easy to use, It allows one-time operation in an experiment instead of frequent operation.
Avoid the stress caused by repeated administration, reducing the impact of non-experimental factors on the experimental results.

62523 62521

62320

0.5~100,0.5~4 0,0.5~5

The osmotic pump has become the best tool for continuous dose of unrestrained laboratory
animals. It can be implanted subcutaneously or intraperitoneally in animals. Minimizes sideeffects and experimental variables, and reduces the risk of stress due to frequent animalhandling. And it is used for continuous delivery of a wide range of drugs, including
antibodies, growth factors, cytokines, chemotherapeutics, addictive drugs, hormones,
steroids and more.
100μL 200μL

2mL

Features:
Ensure continuous and uninterrupted administration, maintaining the preset speed for the administration of the animal.

Notes：(1)For cap, plastic or metal is optional. Metal cap 62525 (corresponding plastic cap 62523), Metal cap 62526 (corresponding
plastic cap 62524).
(2)The difference between Series I and Series III is the height of the pedestal, the former is 7.8mm and the later is 5.5mm, andSeries III is specially designed for mice

Small size, can be applied toexperimentson mice, neonatal rats and other small animal.
Ensure that the drug is delivered to the target site accurately.
The osmotic pump can be directly implanted in the abdominal cavity or the back of the neck for drug administration, or it can be applied to
specific tissue and organ, such as blood vessels, brain, spinal cord, lungs, liver, etc., by connecting the catheter or other accessories.

PE & Silicone Tubings

storage
capsulesflow regulator

PE(Ployethylene) / HDPE(High Density Ployethylene) tube, with good fluidity, high thermal stability and good flexibility, is widely used in
the field of life science. Currently, RWD has 9 types of PE tube and 1 type of silicone tube, including the international standard tubes PE10,
PE20 and PE50, which can be used in microdialysis, animal artery intubation, venous cannula, bile duct intubation, subcutaneous
implantation of buried pipe, lateral ventricle buried pipe, portal vein intubation and so on. These tubes can be used together with syringe,
guide cannula, microdialys connector and luer stubs.

pellicledrug

saltinterlayer

Order Information:
No.

Model

Product Description

Flow regulator: The sole outlet for drug solution, which is used to connect the catheter to target administration area.

162320PE Tubing-0.85*0.42mm,pkg of 10m

Storage capsules: Fill with solution. It has good corrosion resistance to acid and alkali.

262321PE Tubing (HDPE)-0.85*0.42mm, pkg of 10m

362322PE Tubing-0.50*0.25mm, pkg of 10m

462323

PE Tubing-1.00*0.50mm, pkg of 10m

562324

PE10 Tubing-0.61*0.28mm, pkg of 10m

662325

PE20 Tubing-1.09*0.38mm, pkg of 10m

762326

PE25 Tubing-0.91*0.46mm, pkg of 10m

862327

PE50 Tubing-0.97*0.58mm, pkg of 10m

962328

PE60 Tubing-1.20*0.80mm, pkg of 10m

1062329

PE Tubing-1.50*0.50mm, pkg of 10m

Shell: Firmness, designed to protect the internal structure of osmotic pump, with fluid passability characteristics.
Salt interlayer: absorption of tissue fluid by osmosis. After the salt interlayer absorbs the tissue fluid, it can only be extruded inwards toslowly pump the solution out of the capsule.
Pellicle: prevents the salt layer from diffusing into the tissue, and ensures that the tissue fluid can enter the salt layer to squeeze thestorage capsule and pump out the drug solution

Technical Parameters:
2mL

Capsule

Type

1003D

1001W

1002W

1004W

2003D

2001W

2002W

2004W

2ml1W

2ml2W

2ml4W

Duration3d1w

2w

4w

3d1w

2w

4w

1w

2w

4w

Pumping Rate(μL/hr)

1.0

0.5

0.25

0.125

2.0

1.0

0.5

0.25

10.0

5.0

2.5

(10pcs/box)

Animal Administration andBlood Sampling Solution

Infusion tube
(1pcs/box)

PE material

WithoutCap
Draft tube

304 stainless steel material

Syringe

Flat head for the injection of liquid

Animal Administration andBlood Sampling Solution

Flow regulator

Screws

200μL(10pcs/box)

Note:Except62322,otherPEtubecanwithstandhigh-pressuresteamsterilization(121℃,30min).

Model

100μL

Drug capacity

Product DescriptionOsmotic pump kit ordering information:
Screw-M1.0xL2.0mm
Screw-M1.2xL2.0mm
Screw-M1.4xL2.5mm

Brain Infusion Kit

ModelDescription

Bic-3

For 1-3mm deep brain area administration (10 Bags/ Package)

Bic-5

For 1-3mm deep brain area administration (10 Bags/ Package)

Catheter Delivery Kit Mic

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For long-distance muscle administration (10 Bags/ Package)

Different accessory kits

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