Silica gel forColumn Chromatography

Spherical Silica gel for Preparative LCColumn Chromatography
Flash Chromatography

Spherical Silica gel for Column Chromatography
Spherical silica gel are superior to irregular shaped
silica gel in gel sedimentation, and form more uniform
column beds. They have begun to be in wide use forlaboratory-scale purification to industrial-scale
preparative work.

CHARACTERISTICS

Advantages of Spherical Silica gel as Compared to Irregular shaped

Better gel sedimentation

Broader surface area

Uniform column bed

Enhanced
reproducibility

Increased linear velocity

Betterresolution

Promoted
operability

SmallercolumnSelection Guide for Preparative Chromatography● pH of Silica Gel
Pure silica gel (SiO2) is known to be a weak acid fromits surface structure. In instances where samples are
unstable under acidic conditions, pure silica gel may

not be suited for their separation. Our pH-adjustedmaterial, Silicagel 60N, are ideal in separating a wide
range of compounds.

● Particle Size
Particle size

Characteristics

40~50μm

Loose material for high-resolution,flash chromatography. Due tonarrow particle size distribution, easily prepare column beds
with superior in resolution, permeability and uniformity. Suitablefor purification of target substances in complex matrix. Useful asloose material for medium-pressure preparative columns.

40~100μm

Loose material for standard flash chromatography. Due to highpermeability, usable under hydrostatic pressure, depending oneluents. Useful as fillers for high-resolution, small-scale opencolumns.

63~210μm

Economical material for open column chromatography, having
the advantage of easy handling and high cost performance.
Suitable for rough purification. Contribute greatly to reduction inpurification cost in a large-scale preparative operation.

100~210μm

Loose material for standard open column chromatography. Formuniform beds even in large columns. Suitable for mass
preparative chromatography treatment by overload technique.
Due to high permeability and increased throughput, enhances
productivity.

Flash Chromatography

Column Chromatography

Spherial Silica gel for Column Chromatography
Comparison of Resolution
The spherical silica gels are expected to enhance
resolution and reproducibility because they are able toform more uniform column beds.

The separating ability of our spherical Silicagel wascompared with that of irregular shaped silica gel witha similar particle size.
Chromatographic conditions

Products For Flash Chromatography

Column : stainless steel,length 250mm,
inner diameter 10mm
Mobile phase : n-Heptane
Flow rate : 2.5mL/min
Temperature : Room temperature
Detection : UV 254nm
Amount of Injection : 50μL
Sample : 1. Benzene 0.5 mg/mL
2. Naphthalene 0.05 mg/mL

Spherical TypeN Biphenyl = 3.841

Silicagel 60N(Spherical, neutral)

3Irregular TypeN Biphenyl = 2.738
3212Particle size:
40 〜63μm

510152025Particle size:
40 〜50μm

3. Biphenyl 0.015 mg/mL

51015202530

1Products For Column Chromatography
N Biphenyl = 1.898

Spherical Type

Irregular TypeN Biphenyl = 883

Silicagel 60N(Spherical, neutral)
3

31 2

21Particle size:
63 〜200μm
5101520253035

51015202530

Particle size:
63 〜210μm

Particle Size Distribution of Spherical Silicagel
100Weight (%)

80
63~210μm

Products forflash chromatography

100~210μm

60

Products forcolumn chromatography

4040~100μm

40~50μm

200050100150Particle size (μm)

200Ordering information
Product Name
pH (10% sus.)
Particle size (μm)
Cat.No.

Pack size

Silicagel 60 (Spherical)

Silicagel 60N (Spherical, Neutral)

6.0±1.0 (Acidic)

7.0±0.5 (Neutral)

For flashchromatography

For column
chromatography

For flashchromatography

For column
chromatography

40~50

63~210 100~210

40~50

63~210 100~210

40~10040~1001kg

37562-79 37559-79 37564-79 37558-79 37563-79 37561-79 37565-79 37560-79

10kg

37562-84 37559-84 37564-84 37558-84 37563-84 37561-84 37565-84 37560-8425kg

37562-85 37559-85 37564-85 37558-85 37563-85 37561-85 37565-85 37560-85

Physical Properties of Silica gel
Product Name

Silicagel 60 (Spherical)

Silicagel 60N (Spherical, Neutral)

pH (10% sus.)

6.0±1.0 (Acidic)

7.0±0.5 (Neutral)Specific surface area

700±50m2/g

650±50m2/g

Pore diameter65±10Å

60±10Å

Pore volume

1.15±0.10 mL/g

0.90±0.10 mL/g

Bulk density

0.40±0.05 g/mL

0.50±0.05 g/mL

Water content≦ 15.0%≦ 15.0%Cl≦ 100ppm≦ 100ppm

Na≦ 100ppm≦ 100ppm

Mg

≦ 50

ppm

≦ 50

ppm

Ca

≦ 50

ppm

≦ 500

ppm

Al

≦ 150

ppm

≦ 150

ppm

Ti

≦ 150

ppm

≦ 150

ppm

Fe

≦ 50

ppm

≦ 50

ppm

11-5, Nihonbashi-Honcho 3-chome, Chuo-ku, Tokyo
103-0023 Japan
Telephone +813-3639-8301 Telefax +813-3639-9435
http: //www.kanto.co.jp e-mail: reag-info@gms.kanto.co.jp