／【Ｋ：】Ｓｅｒｖｅｒ／１８０１０６５５ 新潟厚生連農業協同組合／本文／３０ａ

厚生連医誌

第２７巻

五十嵐０２８‐０３２

2018.02.26 10.23

１号 ２８〜３２ ２０１８Case report

A case of uterine atypical leiomyoma with excess p５３staining diagnosed as benign by p５
３-Polymerase chain
reaction（PCR）followed by Single-stranded conformationalpolymorphism（p５３-SSCP）in the formalin-fixedparaffin-embedded specimen（FFPE）
Nagaoka Central General Hospital, Department of Pathology,
Department of Genetic diagnosis（Chu-etsu genetic diagnosis study group）; Pathologist

Toshihiko Ikarashi
Background : Generally the macroscopic necrosis, the pathologic atypism, mitotic index（MI）, and p５３stainability are considered to be the reliable histologic diagnostic criteria of cancer. But several
uterine atypical leiomyoma of benignancy could
show marked cellular atypism and p５
３ reaction sothat a further additional study was required to diagnose its malignancy. We had an opportunity tostudy a case of uterine atypical leiomyoma by thegenetic method with p５３-PCR-SSCP and confirm
its benignancy.
Case : ５
４-year-old female patient revealed atypical endometrial hyperplasia and myoma uteri of multiple
leiomyomatous nodules, one intramural nodule ofwhich was finally diagnosed as atypical leiomyoma. It was ４．
２cm in diameter without any
macroscopic necrosis or hemorrhage, but there
were microscopic necrosis surrounded by aggregates of atypically bizarre and multinucleated cells
in leiomyomatous elements. Total count of mitotic
figure （MF） in ６ high power fields of x４００（HPF）with microscopic ocular lens of the field
number（FN）２６．
５, Mitotic index（MI）, was lessthan ５/２mm２. Immunohistochemically there were aslight increment of Ki-６
７-positive cells and abundant p５
３-positive cells. The genomic analysis of p５
３ suppressor gene was done by p５
３-PCR-SSCP
to differentiate atypical leiomyoma from leiomyosarcoma, which failed to show any evidence of genetic anomaly.
Conclusion : No genomic mutation of p５
３ suppressor
gene could disclose this leiomyomatous lesion asbenign.

chain reaction and single-stranded conformational
polymorphism （p５３-PCR-SSCP）, p５
３-SSCP, formalin-fixed paraffin-embedded specimen, immunohistochemistry（FFPE）, Ki-６７

Case report
５４-year-old female patient revealed atypical endometrial hyperplasia and myoma uteri of multiple leiomyomatous nodules, and total abdominal hysterectomy
with bilateral adnexectomy was performed. Postoperative
pathologic study showed atypical endometrial hyperplasia
and myoma uteri with cytologic atypism. This intramural
atypical nodule was ４．
２cm in diameter without any macroscopic necrosis or hemorrhage, but there were microscopic necrosis surrounded by aggregates of atypically bizarre and multinucleated cells in leiomyomatous elements（Fig.１）. Our recent microscope with FN ２
６．
５ required ６
fields to count MI, and the MI of this case was less than
５/２mm２. Immunohistochemically there were a slight increment of Ki-６
７-positive cells and abundant p５３-positivecells（Fig．
２, ３）. The genomic analysis of p５３ suppressor
gene, exon ５−８, was done by p５３-PCR-SSCP to differentiate atypical leiomyoma from leiomyosarcoma, which
failed to show any evidence of genetic anomaly（３, Fig.
４−８）
. In p５３-PCR-SSCP analysis, no genomic mutationof p５３ suppressor gene could disclose this leiomyomatous
lesion as atypical leiomyoma ; a benign counterpart ofleiomyogenic tumor group（Table２,３）.

Key words : uterine atypical leiomyoma, histologic criteria of leiomyosarcoma, excess p５
３ stainability, necrosis, marked cell atypism, mitotic index, ocularlens of the field number （FN）２６．
５, accurate
identification of mitotic figure, p５３-polymerase

Discussion
The histologic findings of malignancy consisted ofnecrosis, atypia, and MI（Table ３）. Macroscopic necrosis
has been regarded as important（Table ３）. Concerning
microscopic necrosis in our case, it was difficult how weshould handle it, and we did not emphasize the signifi-― 28 ―

／【Ｋ：】Ｓｅｒｖｅｒ／１８０１０６５５ 新潟厚生連農業協同組合／本文／３０ａ五十嵐

０２８‐０３２

2018.02.26 10.23

A case of uterine atypical leiomyoma with excess p５
３ staining diagnosed as benign by p５
３-Polymerase chain reaction（PCR）followedby Single-stranded conformational polymorphism（p５３-SSCP）in the formalin-fixed paraffin-embedded specimen（FFPE）

cance of microscopic necrosis.
The MI has been one of the most reliable methods
in diagnosing malignancy histologically, and we should
no longer count the illegible MF-like apoptotic one to improve the precision of MI value（Table １）. On the previous articles before ３０ to ４０ years the total MF in １０HPF
was counted with the ocular lens FN１
５ to ２０, and its total
２ １）. The MI of our case was counted
area was １−２mm（with the ocular lens FN２
６．
５ and we counted ６ HPF（x４００）as the total area of ２mm２ according to some advice
by Miettinen et al （１, Acknowledgement, Table １, ２）.
The MI was less than ５/２mm２, which was short of malignancy（Table３）.
We disclosed the importance of abnormal expression of the suppressor gene p５３ in diagnosing malignancy
histologically（２）. But this benign uterine abnormal leiomyoma also showed excessive p５
３ reaction immunohistochemically, which required further study on genetic mutation of p５３ to differentiate the malignant change（３）. The
p５３-SSCP study was very useful to confirm this case as
benign atypical leiomyoma with the evidence of negative
p５３-PCR-SSCP result（Table３）.

Acknowledgement
Grateful acknowledgement was made to Miettinen,M（NIH/NCI）, and Sasano, K and Kasajima, A（TohokuUniversity）for their advice on searching mitotic counts.

References
１．Ikarashi, T.. Two cases of uterine cellular leiomyoma
being difficult to differentiate from leiomyosarcoma
histopathologically -examination of the mitototic number measurement as the hisopathologic malignant criteria-. Journal of Niigata Society of the Japan Society ofObstetrics and Gynecology１９９１；６２：１３−８．
２．Ikarashi, T. Histological distinction of early adenocarcinoma of both stomach and colon from borderline
malignancies by the immunohistochemical overexpression of Ki-６７ and p５３ proteins. Niigata-ken Koseiren
Med J１９９８；８：３２−７．
３．Hasegawa H and Ikarashi T. Detection analysis of
the p５３ genomic mutations by PCR-SSCP method. Niigata-ken Koseiren Med J２００１；４２−５．

和 文 抄録

症例報告
免疫染色上 p５３が強陽性であった子宮異型筋腫の１症
例−ホルマリン固定−パラフィン包埋材料を使った
p５３-polymerase chain reaction and single-stranded conformational polymorphism（p５３-PCR-SSCP）検査で p５３変異が認められず平滑筋肉腫と鑑別できた１症例−
長岡中央綜合病院、病理科、遺伝子診断室；病理医
いか らしとしひこ

五十嵐俊彦
背景：子宮の平滑筋腫瘍に関して、組織学的悪性基準には、肉眼的壊死、組織学的異型性、細胞分裂像数、免疫染色における p５３強陽性等の所見がある。今回、高度の異型性と p５３強陽性を示す筋腫症例を経験したので、組織学的鑑別と遺伝子補助検査による検討を加えたので報告する。
症例：症例は５４才の女性で、異型子宮内膜増殖症と多発性子宮筋腫で、腹式単純子宮全摘出術と両側付属器摘出術が施行された。多発子宮筋腫の内の４．
２cm 大の筋腫結節関して、顕微鏡的壊死巣とそれを囲繞する高度異型細胞集簇、細胞分裂像数５未満／２mm２相 当、免 疫 染 色 上 p５３強 陽性像が認められた。平滑筋肉腫との鑑別上、ホルマリン固定パラフィン包埋標本（FFPE）を使った p５
３−ポリメラーゼ連鎖反応法−１本鎖DNA 高次構造多型分析法（p５３-PCR-SSCP, p５３SSCP）を実施し、p５３の遺伝子変異が無いことを確認し、異型子宮筋腫と診断した。
結論：子宮異型筋腫と子宮平滑筋肉腫の鑑別において、p５３-PCR-SSCP による p５３抑制遺伝子の変異の同定が有効であった。
キーワード : 子宮異型筋腫、平滑筋肉腫との組織鑑別基準、p５３強陽性、壊死、高度異型、２mm２当りの細胞分裂数、視野番号（FN）２６．
５の接眼レ
ンズ、細胞分裂像の明確な同定、p５３−ポリメ
ラーゼ連鎖反応法−１本鎖 DNA 高次構造多型
分 析 法（p５３-PCR-SSCP、p５３-SSCP）、ホ ル マ
リン固定パラフィン包埋標本（FFPE）、免疫染色、Ki-６７― 29 ―／【Ｋ：】Ｓｅｒｖｅｒ／１８０１０６５５ 新潟厚生連農業協同組合／本文／３０ａ

厚生連医誌

第２７巻

五十嵐０２８‐０３２

2018.02.26 10.23

１号 ２８〜３２ ２０１８Table１. The relationship between Field number of ocular lens（FN）and the area（mm２/field）at using x 40-objective lens㼛㼏㼏㼡㼘㼍㼞㻌㼒㼕㼑㼘㼐 㼒㼕㼑㼘㼐㻌㼍㼞㼑㼍㼚㼡㼙㼎㼑㼞㻌㻔㻲㻺㻕 㻔㼙㼙㻟㻛㼤㻠㻜㻜㻕㻝㻠㻜㻚㻝㻜㻝㻡㻜㻚㻝㻝㻝㻢㻜㻚㻝㻟㻝㻣㻜㻚㻝㻠㻝㻤㻜㻚㻝㻢㻝㻥㻜㻚㻝㻤㻞㻜㻜㻚㻞㻜㻞㻝㻜㻚㻞㻞㻞㻞㻜㻚㻞㻠㻞㻟㻜㻚㻞㻢㻞㻠㻜㻚㻞㻤㻞㻡㻜㻚㻟㻝㻞㻢㻜㻚㻟㻟㻞㻢㻚㻡㻜㻚㻟㻠㻞㻣㻜㻚㻟㻢area (mm2/ﬁeld) at using x40-objecve lens

0.4

0.35

0.3

0.25

0.2

0.15

0.1

0.05

0121416

1820

222426

Field number of ocular lens (FN)

Table２. The number of the necessary microscopic fields necessary for pathologic diagnosis㼍㼠㼥㼜㼕㼍㼞㼑㼝㼡㼕㼞㼑㼐㻌㼏㼛㼡㼚㼠㼕㼚㼓㼏㼥㼠㼛㼘㼛㼓㼕㼏㼍㼘㼒㼕㼘㼑㼘㼐㼟㻌㼎㼥㻌㼡㼟㼕㼚㼓㻌㻲㻺㻞㻢㻚㻡㼔㼕㼟㼠㼛㼘㼛㼓㼕㼏㼍㼘㼜㼞㼕㼙㼍㼞㼥㻌㼛㼞㼓㼍㼚 㼑㼢㼕㼐㼑㼚㼏㼑 㼠㼛㼠㼍㼘㻌㼍㼞㼑㼍㻌㻔㼙㼙㻞㻕㼓㼞㼍㼐㼕㼚㼓㼛㼏㼡㼘㼍㼞㻌㼘㼑㼚㼟㼓㼞㼍㼐㼕㼚㼓㻔㼙㼛㼐㼑㼞㼍㼠㼑㻔㻜㻚㻟㻠㻡㼙㼙㻞㻛㼤㻠㻜㻜㻴㻼㻲㻕 㻔㼙㼛㼐㼑㼞㼍㼠㼑㻌㼓㼞㼍㼐㼑㻕㼓㼞㼍㼐㼑㻕㼎㼞㼑㼍㼟㼠㻶㻿㻯䠪䠡䠰䠳䠤䠫㼟㼛㼒㼠㻌㼠㼕㼟㼟㼡㼑䠳䠤䠫㼡㼠㼑㼞㼡㼟㻘㼟㼙㼛㼛㼠㼔㼟㼠㼛㼙㼍㼏㼔㻘㼟㼙㼛㼛㼠㼔㻶㻿㻯㼟㼠㼛㼙㼍㼔㻘㻌䠣䠥䠯䠰㻹㼕㼠㼠㼕㼚㼑㼚㼕㼚㼠㼑㼟㼠㼕㼚㼑㻘㻌䠣䠥䠯㻶㻿㻯䠰㻹㼕㼠㼠㼕㼚㼑㼚㻞㻢㻞㻢㻜㻚㻝㻣㻟㻠㻛㼤㻠㻜㻜㻴㻼㻲㼤㻝㻜㻴㻼㻲㻩㻝㻚㻣㻠㻟㻲㻺㻩㻞㻜㻔㻝㻥㻥㻜ᖺ௦㻕㼤㻝㻜㻴㻼㻲㻩㻞㻤䡚㻝㻠㻞䡚㻞㻜㻌㻔㻷㼕㻢㻣㻦㻌㻌㻟䡚㻞㻜㻕㻡㻝㻜䡚㻝㻥㻢㻔㼙㼍㼘㼕㼓㼚㼍㼚㼠㻦㻌㻝㻜䍺㻕㻲㻺㻩㻞㻜㻔㻝㻥㻥㻡㻕㻌㻠㻝㻞㻔㼙㼍㼘㼕㼓㼚㼍㼚㼠㻦㻌㻝䍺㻕㻠㻚㻤䡚㻡㻝㻠㻖㻠㻚㻤䡚㻡㻝㻠㻖㻡䡚㻝㻜㻖㼠㼡㼙㼛㼞㼐㼕㼍㼙㼑㼠㼑㼞㼓㼍㼟㼠㼞㼕㼏㻌㻳㻵㻿㼀㻔䠿䡉㻕 䍺㻡㻡䠘䍺㻞㼢㼑㼞㼥㻌㼘㼛㼣㻌㼞㼕㼟㼗㼘㼛㼣㻌㼞㻚䍺㻡㼘㼛㼣㻌㼞㻚㼕㼚㼠㼑㼞㼙㼑㼐㼕㼍㼠㼑㻌㼞㻚䍺㻝㻜㼘㼛㼣㻌㼞㻚㼔㼕㼓㼔㻌㼞㻚㻝㻜䠘㼕㼚㼠㼑㼙㼑㼐㼕㼍㼠㼑㻌㼞㻚㼔㼕㼓㼔㻌㼞㻚㻲㻺㻶㻿㻯㻹㻲㼃㻴㻻㻹㻲㼑㼚㼠㼑㼞㼛㻙㼏㼛㼘㼕㼏㻌㻳㻵㻿㼀䍺㻡㻡䠘㼢㼑㼞㼥㻌㼘㼛㼣㻌㼞㼕㼟㼗㼔㼕㼓㼔㻌㼞㻚㼘㼛㼣㻌㼞㻚㼔㼕㼓㼔㻌㼞㻚㼕㼚㼠㼑㼙㼑㼐㼕㼍㼠㼑㻌㼞㻚㼔㼕㼓㼔㻌㼞㻚㼔㼕㼓㼔㻌㼞㻚㼔㼕㼓㼔㻌㼞㻚㼒㼕㼑㼘㼐㻌㼚㼡㼙㼎㼑㼞㻶㼍㼜㼍㼚㼑㼟㼑㻌㻿㼛㼏㼕㼑㼠㼥㻌㼒㼛㼞㻌㻯㼍㼚㼏㼑㼞㼜㼑㼞㼕㼚㼡㼏㼘㼑㼍㼞㻌㼢㼍㼏㼡㼛㼘㼍㼞㻌㼐㼑㼓㼑㼚㼑㼞㼍㼠㼕㼛㼚㻌㻔㼜㼑㼞㼕㼚㼡㼏㼘㼑㼍㼞㻌㼔㼍㼘㼛㻕㼑㼤㼏㼑㼜㼠㼕㼛㼚 㼍㼜㼛㼜㼠㼛㼟㼕㼟㼞㼑㼒㻦㻌㼕㼙㼙㼡㼚㼛㼟㼠㼍㼕㼚㻌㼛㼒㻌㻷㼕㻢㻣㼐㼕㼒㼒㼕㼏㼡㼘㼠㻌㼙㼑㼍㼚㻌㻔㼙㼕㼚㼕㼙㼍㼘䡚㼙㼍㼤㼕㼙㼍㼘㻌㼕㼚㻌㼔㼛㼠㻌㼟㼜㼛㼠䠅㼞㼑㼜㼛㼞㼠㼃㼛㼞㼘㼐㻌㻴㼑㼍㼘㼠㼔㻌㻻㼞㼓㼍㼚㼕㼦㼍㼠㼕㼛㼚― 30 ―

28

／【Ｋ：】Ｓｅｒｖｅｒ／１８０１０６５５ 新潟厚生連農業協同組合／本文／３０ａ五十嵐

０２８‐０３２

2018.02.26 10.23

A case of uterine atypical leiomyoma with excess p５
３ staining diagnosed as benign by p５
３-Polymerase chain reaction（PCR）followedby Single-stranded conformational polymorphism（p５３-SSCP）in the formalin-fixed paraffin-embedded specimen（FFPE）

Table３. Uterine smooth muscle tumors, histological differentiation of malignancy㻴㼕㼟㼠㼛㼘㼛㼓㼕㼏㻌㼏㼞㼕㼠㼑㼕㼞㼍㻌㼒㼛㼞㻌㼠㼔㼑㻌㼐㼕㼍㼓㼚㼛㼟㼕㼟㻌㼛㼒㻌㼡㼠㼑㼞㼕㼚㼑㻌㼟㼙㼛㼛㼠㼔㻌㼙㼡㼟㼏㼘㼑㻌㼠㼡㼙㼛㼞㼟㼚㼑㼏㼞㼛㼟㼕㼟㼍㼠㼥㼜㼕㼍㼐㼕㼒㼒㼡㼟㼑㻗㼐㼕㼒㼒㼡㼟㼑㼏㼑㼘㼘㼡㼘㼍㼞㼕㼠㼥㼕㼚㼏㼞㼑㼍㼟㼑㼐㼚㼛㼞㼙㼍㼘㼍㼠㼥㼜㼕㼏㼍㼘㻡㻙㻥㻨㻝㻜㼘㼑㼕㼛㼙㼥㼛㼟㼍㼞㼏㼛㼙㼍㻿㼀㼁㻹㻼㻌㻔㻾㻛㻻㼕㼚㼒㼍㼞㼏㼠㼑㼐㼘㼑㼕㼛㼙㼥㼛㼙㼍㻕㼙㼛㼐㻙㼟㼑㼢㼑㼞㼑㼚㼛㻙㼙㼕㼘㼐㼙㼛㼐㻙㼟㼑㼢㼑㼞㼑㻙㼒㼛㼏㼍㼘㻹㻲㻛㻝㻜㻴㻼㻲㻨㻡㼚㼛㻙㼙㼕㼘㼐㼙㼛㼐㻙㼟㼑㼢㼑㼞㼑㼍㼠㼥㼜㼕㼟㼙㼟㼑㼢㼑㼞㼑㼙㼛㼐㼑㼞㼍㼠㼑㼙㼕㼘㼐㼚㼛㼍㼠㼥㼜㼕㼏㼍㼘㼘㼑㼕㼛㼙㼥㼛㼙㼍㻌㼣㼕㼠㼔㼘㼛㼣㻌㼞㼕㼟㼗㻌㼛㼒㼞㼑㼏㼡㼞㼞㼑㼚㼏㼑㼘㼑㼕㼛㼙㼥㼛㼙㼍㼍㼠㼥㼜㼕㼏㼍㼘㼘㼑㼕㼛㼙㼥㼛㼙㼍䠯䠰䠱䠩䠬㻝㻜䍺㼘㼑㼕㼛㼙㼥㼛㼟㼍㼞㼏㼛㼙㼍㼘㼑㼕㼛㼙㼥㼛㼟㼍㼞㼏㼛㼙㼍㼙㼕㼠㼛㼠㼕㼏㼍㼘㼘㼥㻌㼍㼏㼠㼕㼢㼑㻌㼘㼑㼕㼛㼙㼥㼛㼙㼍㻿㼀㼁㻹㻼㻡㻝㻜㼘㼑㼕㼛㼙㼥㼛㼟㼍㼞㼏㼛㼙㼍㼍㼠㼥㼜㼕㼏㼍㼘㻌㼘㼑㼕㼛㼙㼥㼛㼙㼍㻔㼟㼥㼚㼏㼥㼠㼕㼍㼘㻌㼘㼑㼕㼛㼙㼥㼛㼙㼍㻕㼑㼜㼕㼠㼔㼑㼘㼕㼛㼕㼐㻌㼘㼑㼕㼛㼙㼥㼛㼙㼍㼏㼑㼘㼘㼡㼘㼍㼞㻌㼘㼑㼕㼛㼙㼥㼛㼙㼍㼡㼟㼡㼍㼘㻌㼘㼑㼕㼛㼙㼥㼛㼙㼍㻝㻡㻿㼀㼁㻹㻼㻿㼀㼁㻹㻼㼙㼕㼠㼛㼠㼕㼏㼍㼘㼘㼥㻌㼍㼏㼠㼕㼢㼑㻌㼘㼑㼕㼛㼙㼥㼛㼙㼍㻿㼀㼁㻹㻼㼡㼜㼜㼑㼞㻌㼠㼍㼎㼘㼑㻦㻌㼏㼡㼞㼞㼑㼚㼠㻌㼏㼞㼕㼠㼑㼞㼕㼍㻌㼎㼍㼟㼑㼐㻌㼛㼚㻌㼚㼑㼏㼞㼛㼟㼕㼟㻘㻌㼍㼠㼥㼜㼕㼍㻘㻌㼍㼚㼐㻌㻹㻲㻚㼘㼛㼣㼑㼞㻌㼠㼍㼎㼘㼑㻦㻌㼜㼞㼑㼢㼕㼛㼡㼟㻌㼏㼘㼍㼟㼟㼕㼏㼍㼘㻌㼏㼞㼕㼠㼑㼕㼞㼍㻌㼎㼍㼟㼑㼐㻌㼛㼚㻌㼏㼑㼘㼘㼡㼘㼍㼞㼕㼠㼥㻘㻌㼍㼠㼥㼜㼕㼍㻘㻌㼍㼚㼐㻌㻹㻲㻚㼍㼎㼎㼞㻚㻿㼀㼁㻹㻼㼟㼙㼛㼛㼠㼔㻌㼙㼡㼟㼏㼘㼑㻌㼠㼡㼙㼛㼞㻌㼛㼒㻌㼡㼚㼏㼑㼞㼠㼍㼕㼚㼑㼐㻌㼙㼍㼘㼕㼓㼚㼍㼚㼠㻌㼜㼛㼠㼑㼚㼠㼕㼍㼘㻹㻲㼙㼕㼠㼛㼠㼕㼏㻌㼒㼕㼓㼡㼞㼑㼟㼏㼒㻚㻌㼁㼠㼑㼞㼕㼚㼑㻌㼟㼙㼛㼛㼠㼔㻌㼙㼡㼟㼏㼘㼑㻌㼠㼡㼙㼛㼞㼟㻘㻌㼔㼕㼟㼠㼛㼘㼛㼓㼕㼏㼍㼘㻌㼐㼕㼒㼒㼑㼞㼑㼚㼠㼕㼍㼠㼕㼛㼚㻌㼛㼒㻌㼙㼍㼘㼕㼓㼚㼍㼚㼏㼥㻘㻌㼑㼐㼟㻚㻌㻷㼡㼞㼙㼍㼚㻘㻌㻾㻶㻌㼍㼚㼐㻌㻾㼛㼚㼚㼑㼠㼠㻌㻮㻹㻘㻌㻮㼘㼍㼡㼟㼠㼍㼕㼚㻓㼟㻌㼜㼍㼠㼔㼛㼘㼛㼓㼥㻌㼛㼒㻌㼠㼔㼑㻌㼒㼑㼙㼍㼘㼑㻌㼓㼑㼚㼕㼠㼍㼘㻌㼠㼞㼍㼏㼠㻘㻌㻢㼠㼔㻌㼑㼐㻚㻌㼜㼜㻌㻠㻣㻝㻙㻞㻘㻌㻞㻜㻝㻜㻘㻌㻿㼜㼞㼕㼚㼓㼑㼞㻌㻺㼑㼣㻌㼅㼛㼞㼗㻚㻌

Figure１. Marked microscopic cellular atypism（HE）
HE : hematoxylin-eosin stain
bar : １００μm― 31 ―

Figure２. Immunostain of p５３
Marked positive stainability.

㻞㻜／【Ｋ：】Ｓｅｒｖｅｒ／１８０１０６５５ 新潟厚生連農業協同組合／本文／３０ａ

厚生連医誌

第２７巻

五十嵐

０２８‐０３２

2018.02.26 10.23

１号 ２８〜３２ ２０１８

Figure４. Electrophoresis of p５
３products after PCR
PCR : polymerase chain reaction,
CTL : normal control
sample : this case

Figure３. Immunostain of p５３
Magnified view of Figure２.

Figure５. Exon５after p５
３-SSCP
Two same bands were confirmed in both normal control（CTL）and this case（sample）
SSCP : single-stranded conformational polymorphism

Figure６. Exon６after p５
３-SSCP
Two same bands were confirmed in both normal control（CTL）and this case（sample）
SSCP : single-stranded conformational polymorphism

Figure７. Exon７after p５
３-SSCP
Two same bands were confirmed in both normal control（CTL）and this case（sample）
SSCP : single-stranded conformational polymorphism

Figure８. Exon８after p５３-SSCP
Two same bands were confirmed in both normal control（CTL）and this case（sample）
SSCP : single-stranded conformational polymorphism（２０１
７／０５／２１受付）― 32 ―