Product Name:

AllView PAGE Buffer / DynaMarker Protein MultiColor Stable II set
日本語データシート

Code No: DS522

Components
DynaMarker

AllView PAGE BufferProtein MulitColor
Stable II(See page 2)(See page 5)

Code NoDS520S

DM660S

Lot#**************

Size
Storage
Stability

50 mL

100 μL(20x stock solution)
Room temperature*4°C(Protect from light)
2 years at RT

12 months at 4°C*The storage condition of AllView PAGE Buffer (DS520S) is room temperature. Please transfer AllView
PAGE Buffer to room temperature after receiving this set.

1BioDynamics Laboratory Inc.
www.biodynamics.co.jp

Ver.1.4

AllView PAGE Buffer
Description
AllView PAGE Buffer is a new type of running buffer for SDS-PAGE electrophoresis. This buffer has aremarkable feature that enable us to separate proteins with wide range of molecular weights in the basic
Laemmli gel (Tris-HCl) similar to using “gradient gel”. The recommended acrylamide concentration is 6%
for resolving gel and 3% for stacking gel to separate the entire range of about 10 kDa to 250 kDa. Afterelectrophoresis, the polyacrylamide gel can be used directly for CBB staining, silver staining or western
blotting.
Protocol1.

Dilute the AllView PAGE Buffer 20 times with ultrapure water.
(e.g. Add 25 ml of AllView PAGE Buffer to 475 ml of ultrapure water.)2.

Set the gel in electrophoresis chambers.

3.

Fill the chambers with the 1 × AllView PAGE Buffer.

4.

Load your samples and molecular weight markers.

5.

Start electrophoresis.

GelVoltage

Time

Mini gel

250 V

15 min(8 × 10 cm, 1 mm thick)(Constant voltage)

Note: AllView PAGE Buffer is suitable for Laemmli gels (see below “Recommended usage”).
 If precast gels are used, the optimal acrylamide concentration may be different.
 AllView PAGE Buffer is not reusable.
 AllView PAGE Buffer is 20 × stock solution. Please dilute to 1 × solution before use.
 If precipitation of SDS is observed, completely dissolve it in a water bath (about 37°C) before use.
 Optimal electrophoretic time is depending on acrylamide concentration, gel size and voltage.
Accordingly, it is recommended to monitor electrophoresis using a prestained MW Marker(e.g. DynaMarker Protein MultiColor Stable II, Code#DM660).

2
BioDynamics Laboratory Inc.
www.biodynamics.co.jp

Ver.1.4

Recommended usage
The recommended acrylamide concentration is 6% for resolving gel and 3% for stacking gel to separate theentire range of about 10 kDa to 250 kDa. Especially if the separation of low molecular weight proteins (10kDa~30 kDa) is required, 10% resolving gel is recommended.
1.

Gel preparation (Laemmli’s method)

Table 1. Recipes for polyacrylamide resolving and stacking gel (2 mini gels)
Stacking gel (6 ml)

Resolving gel (15 ml)

Gel percentage

3%6%10%Ultrapure water

3.9 ml

8.05

6.05

3.75

3.75

1.5M Tris-HCl (pH8.8)
0.5M Tris-HCl (pH6.8)

1.5

30% Acrylamide/Bis solution

0.6

3.0

5.0

10% SDS

0.06

0.15

0.15

TEMED

0.003 (3 µl)

0.00375 (3.75 µl)

0.00375(3.75 µl)

10% APS

0.06

0.05

0.05

Stacking gel
Resolving gel

3%6%10%kDa*230
141
10071
45
30
25
17
8.4

* DynaMarker Protein MultiColor Stable II, Code#DM660

3BioDynamics Laboratory Inc.
www.biodynamics.co.jp

Ver.1.42.

Electrophoretic image

The mobility of proteins using AllView PAGE Buffer is different from Laemmli electrophoresis runningbuffer (Tris-Glycine-SDS). By using AllView PAGE Buffer® and the Laemmli gels (Tris-HCl), a wide
range of protein sizes can be separated like a gradient gels.
Stacking gel

3%

Resolving gel
Buffer6%

10%

Tris-Glycine-AllView PAGE

Tris-Glycine-AllView PAGE

SDS buffer

BufferSDS buffer

Buffer

230

230

230

230

45
2545

8.4

258.4

* DynaMarker Protein MultiColor Stable II, Code#DM660

4
BioDynamics Laboratory Inc.
www.biodynamics.co.jp

Ver.1.4

DynaMarker

Protein MultiColor Stable IIDescription
The DynaMarker Protein MultiColor Stable II is a pre-stained protein molecular weight marker. The marker hasa remarkable feature that it is possible to store at 4 °C. The feature allow us to start electrophoresis with themarker, because it is always in a liquid state while stored at 4 °C. The DynaMarker® Protein MultiColor Stable
II consists of nine prestained proteins. Each of them are stained red, blue, purple, green or orange, ranging
in apparent molecular weight from approximately 8 kDa to 230 kDa. The DynaMarker Protein MultiColor Stable
II is suitable for visualizing proteins during electrophoresis without staining and for monitoring
electrophoretic transfer onto membranes. The protein concentrations are optimized to give uniform band
intensities. The marker is supplied in gel loading buffer for direct loading onto SDS-PAGE without heatingor adding reducing agents.

－ Myosin－ β-Galactosidase－ Phosphorylase-b－ BSA－ Ovalbumin－ Carbonic anhydrase－ Trypsin inhibitor－ Lysozyme－ Aprotinin

Electrophoresis profile of DynaMarker Protein MultiColor Stable II(10 μl) on 6% polyacrylamide (5% C) Gel / AllView PAGE
Buffer (Code#DS520) as running buffer.

Protocol1.

Take the marker out of refrigerator.

2.

Load 10 μl for mini-gels or more for large size gels.

3.

Load your samples.

4.

Start electrophoresis.

Note: There is no need to heat or add reducing agents.

5
BioDynamics Laboratory Inc.
www.biodynamics.co.jp

Ver.1.4

Contents
Apparent molecular weight (kDa) *Tris-Glycine-SDS buffer

AllView PAGE BufferProtein(Laemmli running buffer)(Code#DS520)

Myosin249

250

β-Galactosidase

137141

Phosphorylase-b

96

100

BSA

73

71

Ovalbumin

4645

Carbonic anhydrase

3130

Soybean trypsin inhibitor

2625

Lysozyme

1817

Aprotinin

6.3

8.4

Note: As covalently bound dye affects protein mobility, each batch of prestained protein marker is calibrated against
unstained standards. A prestained protein marker should be used for approximate molecular weight determination. For
precise molecular weight determination use an unstained molecular weight marker.
* : The apparent molecular weight values are lot specific and depends on the electrophoresis running buffer.

6
BioDynamics Laboratory Inc.
www.biodynamics.co.jp

Ver.1.4