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科目：化学氏名：沼野克俊＜知的財産翻訳検定＞答案用紙★★★

============================================================※以下に解答を記入してください【問１】[Claim 1]
A probe for detecting a site in vivo where a test compound isaccumulated, wherein the probe is represented by formula (I):(S-L)m-B-(F)n(I)wherein S denotes a residue of the test compound; L denotes abiocompatible polymer residue which is capable ofstereospecifically and chemically linking S and B therethrough;F denotes an imaging reagent residue; B denotes a linker whichis capable of linking 1 to 60 Ls and 1 to 10 Fs therethrough ofcapable of linking 1 to 60 Ls; m is an integer from 1 to 60; andn is an integer from 0 to 10.
[Claim 2]
The probe of claim 1 wherein L is an N-(2hydroxypropyl)methacrylamide residue.
[Claim 3]
A transmitting compound comprising a residue of either anoligosaccharide (LNnT) represented by Galβ1-4GlcNAcβ1-3Gal
β1-4Glc or an oligosaccharide derivative having an LNnT motif1

in its structure and a residue of a physiologically active
compound, wherein the transmitting compound is a compound suchthat when being administred to an animal, it is accumulated
specifically in at least one organ selected from the groupconsisting of pancreas, thymus gland, orchis and prostate,
thereby delivering the physiologically active compound to theorgan.

[0011]The therapeutic effectiveness of a pharmaceutical varies
depending upon the amount of a drug arriving at an in vivo sitewhere the drug acts, the pharmacokinetics of the drug and thesensibility of the body to the drug.

The exertion of efficacy

at a non-active site may result in a side effect.

Site-specificdrug delivery (henceforth, referred to as targeting) is capableof not only enhanceng the efficacy through increase in drugconcentration at the target site but also relieving such sideeffects.
[0012]
Examples of a technique of drug targeting include a method using
prodrugging and a method using a drug carrier.

The former isa technich in which a drug is chemically modified so that thedrug will change to an activated substance in the active site
environment, whereas the latter is a technique in which a drug
is linked to a device which is capable of derivering the drug2

specifically to an active site.

The method using a carrier

includes active targeting and passive targeting.

In the active

targeting, a ligand which exhibits affinity to an active site
is added to a carrier.

In the passive targeting, a carrier isprovided with a physicochemical property such that theaccumulation of a drug at a non-active site is avoided.

In

general, in comparison to the passive targeting, the active
targeting can achive a higher selectivity of drug distribution
in the body and can obtain a high efficacy in a lower dose.
[0021]
Compound (b) may also be prepared by allowing compound (a) toreact with an acid anhydride such as succinic anhydride andglutaric anhydride in the presence of base the same as thatmentioned supra.

Compound (b) can be purified and isolated in

an arbitrary purity by conventional techniques such as anion
exchange chromatography, hydrophobic chromatography, reversed
phase chromatography, two-phase distribution andrecrystallization.
[0031]
Compound (C) can be obtained also by dissolving or dispersing
compound (b) in an appropriate solvent such as dichloromethane,
N,N-dimethylformamide, dimethyl sulfoxide, toluene,tetrahydrofuran and acetonitrile containing a greatly excessive
molar equivalents of alcohol such as methanol and ethanol, andallowing the compound (b) to react with 1-30 molar equivalents3

of trimethylsilyldiazomethane at a temperature of from -20 to150degC for a period of time of from one hour to ten days.
[0041](Step 4)
Oligosaccharide A (LNnT), oligosaccharide B (6'-SLNnT) andorigosaccharide D (O-acetyl-LNnT) were prepared according to themethod disclosed in WO99/40205.

As oligosaccharide C (lactose:Galβ1-4Glc), a commercially available product (available fromKokusan Chemical Co., Ltd.) was used.

Compounds 4A, 4B, 4C and4D were obtained by introducing compound 3 into oligosaccharides
A, B, C and D, respectively, according to the method disclosedby Cabacungan et al. [Analytical Biochemistry, Vol. 124, pp272-278 (1982)].

Compounds 4A, 4B, 4C and 4D were purified bypassing them through a tower Sephadex G-25 (manufactured byAmersham Pharmacia Biotech Co.) in which the reaction solution
was put in equilibrium using water.

【問２】(ii) two groups present on the same carbon atom forms a saturated
carbon ring together with the carbon atom within R1, R2, E3 andR4;(iii) two groups present on two carbon atoms adjacent to eachother form a saturated carbon ring together with the carbon atoms
within R1, R2, R3 and R4; or(iv) two groups present on carbon atoms adjacent to each other4

represent a bond together within R1, R2, R3 and R4 (the groupsform a double bond together with an existing bond).

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