Reagents for Dioxins Analysis
Analysis of Dioxins including coplanar PCBs is not easy because
target compounds have many isomers and concentrations areextremely low. In Japan, method for measuring coplanar PCBs is
specified in JIS and other research manuals. This method is essential
for Dioxins analysis. For Dioxins analysis, a variety of pretreatment
methods are used to isolate target compounds from other stuff(impurities).

Sorbent for Sample Preparation of Dioxins
Kanto chemical has developed “Active carbon-dispersed silica gel” as achromatographic adsorbent for pretreatment of dioxin analysis. This product isperfect pretreatment filler for dioxins analysis utilizing adsorption properties ofactive carbon and silica.
“Active carbon-dispersed silica gel” is a very useful pretreatment filler for
highly accurate measurement.

Feature◆ Separation of Dioxins and coplanar PCBs is achieved.

◆ Less variation between lots due to uniform particle size.

◆ Reduction in blank values has been successfully achieved by special treatment.
So prior cleaning is unnecessary.
Product NamePackageGradeProduct No.

Active carbon-dispersed silica gel10 gfor dioxins analysis

01875-43

Example of usage for “Active carbon-dispersed silica gel”

【1】Column packing①Column tube for chromatography
glass tube, inner diameter : 10 mm, length : 300 mm②Active carbon-dispersed silica gel
1 g/ column③Packing
Place cotton wool or quartz glass wool at the bottom of column, then place
anhydrous sodium sulfate (about 10 mm), Active carbon-dispersed silica gel 1g,and again anhydrous sodium sulfate (about 10 mm).

【2】Sample preparation④Preparation of sample solution
a. Concentrate sample solution to near dryness to remove toluene
b. Dissolve toluene-free sample in about 1 ml of hexane.
⑤Loading of sample on the column
Load sample solution onto the column of Active carbon-dispersed silica gel andallow compounds to be absorbed.

【3】Fractionation/Measurement⑥Fraction 1
a. Hexane 50-70ml is passed through the column.
b. Amount of hexane may vary depending on the sample.
c. Fraction 1 contains non-target PCBs. Dioxins (including coplanar PCBs) are not
eluted in this fraction.
⑦Fraction 2
a. Dichloromethane/hexane (1 : 3) 40 ml is passed through the column.
b. Mono-ortho PCBs are eluted in Fraction 2.
⑧Fraction 3
a. Toluene 200 ml is passed through the column.
b. Non-ortho PCBs, dioxins are eluted in Fraction 3.
⑨
Concentrate Fraction 3 (toluene solution) to form sample for GC-MS.
Note The procedure described above is merely an example.
There are a wide variety of samples for Dioxins analysis.
Prior to use, checking the use condition is recommended.

Example of Elution Profile for “Active carbon-dispersed silica gel”

Solvents for Analysis of Dioxins “Guaranteed by x10,000 Concentrated Solvent”★Blank test of Solvent “Guaranteed by x 10,000 Concentrated Solvent” is conducted.
Blank test of each Solvent “Guaranteed by x 10,000 Concentrated Solvent” by GC/MS(SIM) showsthat 4-8 chlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs) are below 10μg/L(10ppb).
→ below 1ng/L(1ppt) as product (before concentration)

Product Name

AcetoneDichloromethane
Ethanol
HexaneMethanolToluenePackage

GradeProduct No.

1L

for dioxins analysis

01025-79

3L

for dioxins analysis

01025-76

1L

for dioxins analysis

11338-79

3L

for dioxins analysis11338-76

1L

for dioxins analysis

14039-78

3L

for dioxins analysis

14039-76

1L

for dioxins analysis

18636-79

3L

for dioxins analysis

18636-76

1L

for dioxins analysis

25184-79

3L

for dioxins analysis

25184-76

1L

for dioxins analysis

40500-79

3L

for dioxins analysis

40500-76

Solvents for Analysis of Dioxins “Guaranteed by x100,000 Concentrated Solvent”
As solvents for analysis of ultratrace Dioxins in water, food, and living organisms,
extremely low blank values are achieved.
★Blank test of Solvent “Guaranteed by x 100,000 Concentrated Solvent” is conductedby HRGC/HRMS.
Blank test of each Solvent “Guaranteed by x 100,000 Concentrated Solvent” by HRGC/HRMS showsthat 4-8 chlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and coplanar PCBs are below
detection limit.

★Inspection Report issued by Chemicals Evaluation and Research Institute, Japan(CERI) is attached.
Inspection Report issued by Chemicals Evaluation and Research Institute, Japan (CERI), which
carries out blank test, is attached for every product (1L×6).
Product Name

PackageGrade

Product No.

Acetone1Lfor dioxins analysis

01025-97

Dichloromethane1Lfor dioxins analysis

11338-97

Hexane1Lfor dioxins analysis

18636-97

Toluene1Lfor dioxins analysis

40500-97

Example of Test Results for Solvents for Dioxin Analysis (GC/MS Chromatogram)

Chromatogram of “Guaranteed by x 10,000
Concentrated Toluene”

Chromatogram of Dioxins mixed standard solution（each 10ppb)

Analysis condition GC/MS•
•

•
•
•
•
•
•
•
•
•

：HP6890／HP5972：HP-5MS（5%Phenylmethylsiloxane）
Length：30m、I.D.：0.25mm、Film thickness：0.25μm
Carrier Gas：He 1ml/min
Column Temp.
：100℃（1min） ～20℃/min～200℃（0min）～5℃/min～300℃（4min）
Injection Volume：5μl Pulsed splitless（Purge time：1.5min）
Injection Temp.
：300℃
Interface Temp.
：280℃
Detection：MSD(SIM)Temp. of Ion Source ：180℃
Time of Solvent Cut ：6min
SIM Condition:
Apparatus
Columntime

MonItor Ion① TCDD、 ② PeCDD、 ③ HxCDD、 ④ HpCDD、 ⑤ OCDD
⑥ TCDF、 ⑦ PeCDF、 ⑧ HxCDF、 ⑨ HpCDF、 ⑩ OCDF

Active carbon-dispersed silica gel reversible column

PCDDs,PCDFs, Co -PCBs
Perfect column for clean-up procedure of Dioxins
This reverse column shows excellent separation characteristics
for clean-up process of Dioxins analysis, utilizing active carbon
properties, which specifically absorb planar compounds such asdioxins. Prior cleaning which requires complicated operation is nolonger necessary. We have successfully removed potential
interferences from this column by special pretreatment technology.

Separatory funnel◆ Excellent Separation Results
By utilizing active carbon properties, which specifically absorb
planar compounds such as dioxins, this reverse column shows
excellent separation characteristics.

◆ Reduction of Blank
Potential interferences have already been removed by heat
treatment under nitrogen gas. You can use the column without priorcleaning.

Reversible◆ Reduction of solvents usage
Back flush method is used. Thus, amount of solvents used issignificantly reduced compared with conventional analytical method.

Teflon straight union◆ Easy Storage
Unlike alumina which shows varied fraction profile depending on
activities, this product with stable profile is easy to store andhandle.

◆ Specified sample injection direction
Sample load direction is printed on the column for the convenienceof back flush use.

Active carbon-dispersed
silica gel reversible column

Example of usage for “Active carbon-dispersed silica gel reversible column”
Sample : hexane solution
Sample introduction : After adding sample,
wash with small amount of hexane and allow tostand for about 30 minutes.

Fraction 1

Fraction 1 : PCBs other than non and monoorthoPCBs are removed with 30-40 ml ofhexane.
Fraction 2 : Mono-orthoPCBs are eluted with
25-40ml of 25vol% dichloromethane/hexane.
Fraction 3 : Reverse the direction of column
and then elute non -orthoPCBs,
PCDDs/PCDFs with 30-60ml of toluene.

Fraction 2

Fraction 3

Results of recovery test
Added

FoundRecovery

AddedFoundRecovery

Separatory funnel

Reserver for reversible column

Product Name

Teflon straight union

Package

Product No.

5 pieces/pack01894-96

Reserver for reversible column TS19/28

1 pack96942-10

Reserver for reversible column TS15/25

1 pack96942-11

Reserver for reversible column SPC19

1 pack96942-12

Separatory funnel 200mL TS15/25

1 pack96942-15

Separatory funnel 200mL TS19/28

1 pack96942-14

Teflon straight union

1 pack96942-13

Active carbon-dispersed silica gel reversible column(202307)