EpCAM Antibody / Cytoplasmic domain [clone EGP40/1110] (V2686)
Catalog No.

Formulation

Size

V2686-100UG

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

100 ug

V2686-20UG

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

20 ug

V2686SAF-100UG

1 mg/ml in 1X PBS; BSA free, sodium azide free

100 ug

V2686IHC-7ML

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC
use only*

7 ml

Bulk quote requestAvailability

1-3 business days

Species Reactivity Human, Mouse, Rat, Dog, Cat
FormatPurifiedClonality

Monoclonal (mouse origin)

Isotype

Mouse IgG2b, kappa

Clone Name

EGP40/1110

PurityProtein G affinity chromatography

UniProt

P16422

Localization

Cell surface, cytoplasmic

Applications

Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Limitations

This EpCAM antibody is available for research use only.

IHC: Formalin-fixed, paraffin-embedded human ovariancarcinoma stained with EpCAM antibody (cloneEGP40/1110).

IHC: Formalin-fixed, paraffin-embedded rat Oviductstained with EpCAM antibody (clone EGP40/1110).

IHC: Formalin-fixed, paraffin-embedded rat colon
stained with EpCAM antibody (clone EGP40/1110).

IHC: Formalin-fixed, paraffin-embedded rat lungstained with EpCAM antibody (clone EGP40/1110).

IHC testing of FFPE mouse colon tissue with EpCAM
antibody (clone EGP40/1110). Required HIER: boil
tissue sections in 10mM Citrate buffer, pH 6.0, for
10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt(TM) microarray containing morethan 19,000 full-length human proteins using EpCAM
antibody (clone EGP40/1110). These results
demonstrate the foremost specificity of theEGP40/1110 mAb.
Z- and S- score: The Z-score represents the strength ofa signal that an antibody (in combination with afluorescently-tagged anti-IgG secondary Ab) produces
when binding to a particular protein on the HuProt(TM)array. Z-scores are described in units of standard
deviations (SD's) above the mean value of all signals
generated on that array. If the targets on theHuProt(TM) are arranged in descending order of theZ-score, the S-score is the difference (also in units ofSD's) between the Z-scores. The S-score therefore
represents the relative target specificity of an Ab to its
intended target.

SDS-PAGE analysis of purified, BSA-free EpCAM
antibody (clone EGP40/1110) as confirmation ofintegrity and purity.

IHC staining of FFPE dog colon with EpCAM antibody(clone EGP40/1110). HIER: boil tissue sections in pH9
EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cat small intestine with EpCAM
antibody(clone EGP40/1110). HIER: boil tissue sections
in pH9 EDTA for 20 min and allow to cool before
testing.

Description
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial
cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast
majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma andhepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Application Notes
Optimal dilution of the EpCAM antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed bycooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (ifrequired), drip mAb solution onto the tissue section and incubate at RT for 30 min.

ImmunogenA portion of amino acids 289-314 (cytoplasmic domain) was used as the immunogen for the EpCAM antibody.

Storage
Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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