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Websitewww.genecopoeia.com

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Distributors
Australia & New ZealandUnited Bioresearch Products

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India

Life Technologies (India) Pvt. Ltd.

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IsraelORNAT Biochemicals andwww.ornat.co.ilLaboratory Equipment Ltd.
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GeneCopoeia Products are for Research Use Only
Trademarks: GeneCopoeia , miExpress , miArrest , TiterBoost , Lentifect ,Lenti-Pac , EndoFectin , OmicsLink (GeneCopoeia Inc.)
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Copyright © 2011 GeneCopoeia Inc.
LENTI-022311

Lentiviral Solutions

Expression-Ready Clones
Lentiviral-based Expression Clones
ORF cDNA, shRNA, miRNA, Inhibitor
Packaging Systems
Lenti-Pac Lentiviral Packaging Kits
293Ta Packaging Cell Line
EndoFectin Transfection Reagents
TiterBoost Viral Titer Reagent
TM

TMTM

Viral Particles
Lentifect Lentiviral Particles
Catalog or Custom OrderTM

Why Lentivirus
High efficiency of delivery to broadcell types
The lentiviral expression system is very effectiveat delivering genetic materials to whole modelorganisms and a wide range of mammalian cells,
including non-dividing and difﬁcult-to-transfect cells,such as neuronal cells, primary and stem cells.
The efficiency of lentiviral transduction is close to100%, making it an ideal delivery system for genes,
shRNAs, miRNAs and other genetic materials.

Long-term stable expression of atransgene
The lentiviral expression system consists ofsequence elements allowing efficient packaging,transduction and stable integration into the hostgenome of target cells, thus, enabling long-termhigh level of transgene expression in target cells.

High level of safety
Lentiviral vectors used for gene transfer are
replication-defective and self-inactivated intransduced cells. Multiple packaging plasmids
are needed for packaging replication-defective
lentiviral particles and the chance of recombination
to form complete replicable competent lentivirus(RCL) is minimal.

Figure 1. Illustration of how lentiviral-based genedelivery works. Note: Target cells include difﬁcultto-transfect and non-dividing cells.

GeneCopoeia Complete Lentiviral Solutions

1Expression-ready clones

Packaging kits and cell line

● OmicsLinkTM ORF cDNAClones● OmicsLinkTM shRNA Clones● miExpressTM miRNA
Precursor Clones● miArrestTM miRNA InhibitorClones● Lenti-PacTM Lentiviral
Packaging Kits
● Lenti-PacTM 293Ta
Packaging Cell Line
● EndoFectinTM Transfection
Reagent

Transduction-ready viralparticles● Premade lentiviralparticles● Custom lentivirus
production services

Expression-Ready Clones
GeneCopoeia provides a large collection of lentiviral constructs for expressing ORF cDNAs, shRNAs,
precursor miRNAs, or miRNA inhibitors in virtually all mammalian cell types.

OmicsLinkTM ORF cDNA clones● Fully sequenced and expression -tested● Human and mouse genome-wide coverage● Custom order of de novo gene synthesis● Largest selection of vectors and fusion tags

Promoter

Selection MarkerFusion tag (N- and/or C-terminus)

CMV
CMV5
EF1a
CAG
PDK
SV40
Inducible

Neomycin
Puromycin
Hygromycin
Zeomycin

HA, 3xHA
Flag
Myc
eCFP
eGFP
eYFP
mCherry
UBC9HaloTag*
AviTag
AviTag + IRES-Biotin
ligaseIRES-eCFP
IRES-eGFP
IRES-eYFP
IRES-mCherry

IRES-FLuciferase(Firefly)
IRES-GLuciferase(Gaussia)
IRES-Neomycin
IRES-Puromycin

HA + IRES-eGFP
Myc + IRES-eGFP
Flag + IRES-eGFP*Tev Protease site

OmicsLinkTM shRNA clones● Genome-wide coverage of human, mouse and rat● Four shRNA constructs per target gene and guaranteed knockdown for at least one construct● H1 or U6 promoter
Promoter

Selection marker

Reporter gene

H1, U6

Puromycin, Hygromycin, Neomycin

eGFP, mCherry

miExpressTM Precursor miRNA clones● Fully sequenced and optimized for high expression and maturation of miRNA inside cells● Full coverage of human, mouse and rat miRNA in miRBase database
Promoter

Selection marker

Reporter gene

H1, CMV, EF1α

Puromycin, Hygromycin, Neomycin

eGFP, mCherrymiArrestTM miRNA Inhibitor clones● Superior potency, long-lasting inhibition and extremely low cell toxicity● Full coverage of human, mouse and rat miRNA in miRBase database
Promoter

Selection marker

Reporter gene

H1, U6, CMV

Puromycin, Hygromycin, Neomycin

eGFP, mCherry2

Packaging Systems
The GeneCopoeia Lenti-Pac™ Packaging Systems provide high titer (up to 1010 copies/ml), efﬁcienttransduction and superior level of protein expression. Developed using GeneCopoeia lentiviral
expression-ready clones, Lenti-Pac™ Packaging Kits include an optimized lentiviral packaging plasmid
mix, eGFP control clone, EndoFectin™ Lenti, a transfection reagent developed to work with lentiviralbased constructs, and TiterBoost™, a proprietary reagent that further increases titer by 5-10 folds.

ProductDescription

Lenti-PacTM Packaging Kit

Lentiviral packaging mix
eGFP control clone
EndoFectin™ Lenti transfection reagent
TiterBoost™ viral titer reagent

Lenti-PacTM 293Ta Lentiviral
Packaging Cell Line

Low passage and authenticated
1.5 x 106 cells

GeneCopoeiaTM GCI-L3 ChemicallyCompetent E. coli Cells

High transformation efﬁciency with extremely low rates ofrecombination

EndofectinTM Lenti Transfection
Reagents

Optimized for use with GeneCopoeia lentiviral expression
systems. Transfect efﬁciently and with low toxicity

EndoFectinTM Lenti

Lipid-based reagent

Figure 2. Comparison of Transfection Reagents HEK293T
packaging cells were transfected with GeneCopoeia Lentiviral
vector expressing mCherry fluorescent protein and Lenti-Pac
HIV packaging mix using either EndoFectin Lenti (left panel),or a leading lipid-based transfection reagent (right panel).
Expression of mCherry and cell health were checked 48
hours post-transfection using fluorescence microscopy (upperpanel) or phase contrast microscopy (lower panel). The titer oflentivirus generated with EndoFectin Lenti reagent was over20-fold higher than with the lipid-based transfection reagent.

Lentivirus
ControlABC

32 µl

8 µl

Figure 3. Comparison of Packaging Mixes H1299 cells weretransduced with eGFP expressing lentiviral particles generated
with three different packaging mixes:
A: GeneCopoeia’s Lenti-Pac packaging mix
B: LX packaging mix
C: UM packaging mix

Lentiviral Particles
Lentiviral vectors are potent vehicles for delivering genes into a wide range of cell types including
difﬁcult-to-transfect and non-dividing cells. However, producing, concentrating and titrating lentiviralparticles are time consuming and require experience to achieve high titers and consistent results.
GeneCopoeia’s experienced scientiﬁc expertise consistently produces high quality and high-titer crudeor puriﬁed viral particles to meet your research need in an efﬁcient and cost-effective way.

ProductGene

Premade iPSC geneparticles● Induced pluripotent stem cell Transduction-ready(iPSC) genes -Oct3/4, Sox2, Up to 109 copies/ml, crude or puriﬁed
c-Myc, Klf4, Nanog and Lin28 Available in 200 µl and 1 ml sizes

Premade control
particles● Positive controls-eGFP,mCherry, Firefly luciferase,
Renilla luciferase● Negative control -no insert

Custom lentiviralproduction services

DescriptionTransduction-readyUp to 1010 copies/ml, crude or puriﬁed
Available in 200 µl and 1 ml sizes● Any GeneCopoeia
expression-ready clones
of ORF cDNAs, shRNAs,
Choices of lentiviral vectors with or
precursor miRNAs and miRNA without tags
inhibitors
Custom-made vectors also available
10● De novo gene synthesis and Up to 10 copies/ml, crude or puriﬁed
Available in 200 µl and 1 ml sizes
cloning services for genes
not listed on GeneCopoeia
websiteFigure 4. Transduction of H1299 cells withGeneCopoeia lentiviral particle expressing alarge gene.
H1299 cells (in 24-well plate) were transducedwith indicated amounts of LP-Y3533-Lv122
in the presence of 5 µg/ml of polybrene. The
expression of C-terminal eGFP SMARCA4fusion protein was checked with a fluorescencemicroscope 72 hours post-transduction.
ID of Y3533: SMARCA4
Length of SMARCA4 coding region: 4944 bp
Length of SMARCA4 eGFP fusion: > 5.6Kp

0 µl

5.0 µl

10 µlCount on us● High titer crude or purified lentiviralparticles● Stringent quality control and qRT-PCRvalidation● Fast delivery● Cost effective

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