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GeneCopoeiaTM Products are for Research Use Only
Trademarks: GeneCopoeiaTM, OmicsLinkTM, ORFEXPRESSTM, LentifectTM, EndoFectinTM, Lenti-PacTM,
miExpressTM, miArrestTM, miTargetTM, All-in-OneTM, RecJoinTM (GeneCopoeia Inc.), AviTagTM (Avidity), HaloTag®
(Promega), Gateway® (Life Technologies)

Copyright © 2011 GeneCopoeia Inc.
ORF-021511

ORF cDNA Expression Clones

Expression-Ready ORF cDNA Clones
OmicsLinkTM ORF cDNA Clones

Tag Technologies

Fusion Tags: Singular and Tandem
OmicsLinkTM Anti-Tag Antibodies

Expression Systems

Mammalian, Lentiviral, Bacterial, Yeast, Insect,
Wheat Germ Cell Free

ORF cDNA Shuttle Clones

ORFEXPRESS Gateway® PLUS Shuttle Clones
TMFree from Cloning
Save time and resourcesOver-expression of genes and proteins is widely used infunctional genomics, proteomics and system biology studies.
However, generation of expression clones frequently requires
multi-step cloning processes as well as lengthy verificationand sequence analysis. Also, when proteins of interest
are hard to produce in heterologous systems or cannot be
purified by standard methods, or need to be visualized forsubcellular localization, etc., custom vectors and codon
optimization are often needed. Therefore, it can easily takeyou weeks or even months to build the final constructs thatmeet your needs.

Approach 1
Start protein expression rightaway using expression-readyORF cDNA clones

Let GeneCopoeia do the work for you
so you can focus on other challenging
tasks. GeneCopoeia provides wide
choices of expression systems,
promoters, fusion tags and selection
markers. Researchers can either
request to have their gene of interest
cloned into a chosen vector, or simply
order from GeneCopoeia premade
expression-ready ORF cDNA clone
collection. All constructs are fully
sequence-verified and ready-forexpression.

Approach 2
Start cloning using ORF cDNA
shuttle clones

Approach 3
Start cloning using full-length
cDNA template clones

Template cDNA
Gateway® Plus
shuttle clone

OmicsLinkTM
expression-ready clone

Destination
vectorEmpty
vectorOR

Restriction enzyme
digestion and ligation

Recombination

PCR
Protein coding
ORFs

Empty
vectorRestriction enzyme
digestion and ligation

Digested
vectorInsertTransformation
Ligation
Clone selection

Transformation

Validation

PCR

Clone selection
Validation

Restriction
enzyme digestion

PCR

Sequencing

Restriction
enzyme digestion
Sequencing

Expression-readyclonesMultiple applications

Best Value: No work

Application

Fair Value: Some work

Figure 1. Clone selection guide: Expression-ready vs. other types

1

Expression-readyclonesApplication

Less Value: Lots of work

Expression-Ready
OmicsLinkTM Expression-Ready ORF cDNA Clones
OmicsLink expression-ready ORF cDNA clones represent
the largest collections (over 45,000) of human and mousefull-length protein-coding ORF cDNA clones. They are
optimized for protein expression, easy purification andfunctional assays in a variety of cell systems .

OmicsLinkTM expression-ready ORF cDNA clones vs. other clone types

FeatureExpression-Ready ORF
cDNA Clone

ORF cDNA Shuttle Clone

Whole Transcript cDNA
Template Clone

Product

OmicsLink™ expressionready ORF cDNA clones

ORFEXPRESS™ Gateway®
PLUS shuttle ORF cDNA clones

GeneCopoeia full-length
cDNA clones

ProteinexpressionreadyYes

No, need several subcloning
steps

No, need many subcloning
steps (labor intensive)

cDNA insert

ORF only, no 5’ and 3’ UTRs

ORF only, no 5’ and 3’ UTRs

Full-length cDNA with 5’
and 3’ UTRs

Promoter

Numerous promoter choices

No promoter

No promoter

Tag

50+ tag choices

No tag

No tagSelection marker

Various choices

No selection marker

No selection marker

Ribosomalbinding site

Yes (optimized)

Yes (optimized)

Yes (original)

Coding region
fully sequenced

Yes

Yes

Yes

Vector type100+ vector choices for
mammalian, bacterial, yeast,
insect, lentiviral, cell freesystems

ORFEXPRESS - Gateway®
PLUS vector (with additional
MCS) for recombination or
traditional cloning

Non-expression, nonshuttle vector
Traditional cloning only, no
recombination cloning

Need fordestination
vector

Not required, expressionready

Need destination expression
vectorNeed empty expression
vector2

Widest Choices
SourceOmicsLink expression-ready ORF cDNA clones are generated from sequence validated full-length cDNA
clones or high quality cDNA libraries. They are constructed in expression vectors using GeneCopoeia’s
proprietary RecJoinTM cloning technology. With widest choices of over 100 expression vectors and 50 singularor combinational tandem fusion tags, researchers have the flexibility to find the most suitable constructs fortheir tasks in functional genomics, proteomics, and system biology research. They can either request to havetheir gene-of-interest cloned into a chosen vector with unique features or simply order from the large collectionof GeneCopoeia premade human and mouse expression-ready ORF cDNA clones.

Widest choices of expression systems■ Bacterial (14 vector types)■ Mammalian (35 vector types)■ Lentiviral (48 vector types)■ Yeast (3 vector types)■ Insect (1 vector type)■ Wheat germ cell free (8 vector types)

Mammalian

Bacterial

Lentiviral
OmicsLink™
ORF cDNA
Vector
Systems

Yeast

InsectFull genome coverage
Cell Free■ Human■ Mouse■ Zebra fish

100+ vectors with choices of promoters, fusion tags and selection
markers
Promoter

CMV, T7, Tac, EF1α, GAL1, pADH, AcMNPV polyhedrin, custom promoter

Selection marker

Neomycin, puromycin, hygromycin, blasticidin, zeocin

Fusion tag• Fluorescent tags:• Multifunctional tags:• Solubility and purification tags:• Antibody immunoprecipitation tags:• IRES- coexpressed proteins:• And more

Vector type3

Lentiviral and non-viral vectors

eGFP, eYFP, eCFP, mCherry
HaloTag®, AviTag™
His6, SUMO, Flag, GST, MBP, 3xFlag
3xHA, Myc, Flag, 3xFlag
Avi+IRES-Biotin ligase, Myc+IRESeGFP, IRES-eGFP, IRES-Neomycin,
IRES-Luciferase, etc.

Versatile Solutions
Applications■ In vivo and in vitro gene over-expression■ Functional studies using model cell lines or wholemodel organisms■ Cellular imaging for protein trafficking, localization,
immobilization (Fig. 2)■ Transduction into stem, primary and other difficult-to-transfect cells (Fig. 3)■ Functional rescue in shRNA and miRNA studies■ High-throughput screening assays■ Protein-DNA and protein-protein interaction studies■ Protein expression and production

Guarantee
GeneCopoeia guarantees that all full-length OmicsLink
expression clones are free of artificially generated
point mutations and frame-shifting mutations including
deletions and insertions as well as translation
termination mutations (point mutations that result in apremature stop codon).
■ All ORFs are fully sequenced■ PCR amplification and size validation■ Enzyme digestion check of the integrity of wholeplasmid

Figure 2. Live cells expressing HaloTag ® fusion
protein labeled with three different ligands. HeLa
cells transiently transfected with HaloTag ® pHT2
expression clones were labeled with 5 μM HaloTag®
TMR Ligand (Panel A); 10μM HaloTag® diAcFAM
Ligand (Panel B); or 25 μM HaloTag ® Coumarin
Ligand (Panel C).

Advantages

Figure 3. Transduction of H1299 cells with GeneCopoeia
lentiviral particles expressing a large gene.
H1299 cells (in 24-well plate) were transduced withindicated amounts of LP-Y3533-Lv122 in the presence of
5 µg/ml of polybrene. The expression of C-terminal eGFPSMARCA4 fusion protein was checked with a fluorescencemicroscope 72 hours post-transduction.
ID of Y3533: SMARCA4
Length of SMARCA4 coding region: 4944 bp
Length of SMARCA4 eGFP fusion: > 5.6Kp■ Ready-to-transfect and ready-toexpress■ Test multiple expression systems toachieve the best protein expression
at affordable price● Up to 80% discount on additional
expression constructs in different
vectors for the same gene accession
number*● Overcome difficulties in expression,
production and purification withchoice of 100+ expression vectors■ Fully sequence-verified*Contact us for details

4Powerful Tags
Fusion tags
GeneCopoeia offers more than 50 singular and tandem tags to meet your expression, purification,
visualization, detection and localization needs.

Fusion tag

Purification

Increasesolubility

Ab-IP

His

+

+/-

+

Sumo/His-Sumo

++

++

+

GST

+

+

+

MBP

+

++

+

Flag/3xFlag

+

+/-

+

Cellularlabeling

Fluorescent

+

3xHA

+

++

eGFP/eCFP/eYFP/mCherry

+++

+++

cMyc

+

+

AviTag™

+++++

His-AviTag™

++

++

++

HaloTag®

++

++

+++

++

AviTagTM Technology
The AviTag technology is based on the highly specificbiotinylation of the 15 amino acid AviTag by biotin
ligase in vitro or in vivo and on the specific andreverse binding of avidin or streptavidin to biotin forimmobilizing, purifying and visualizing proteins.

Applications■ Purify AviTag-fusion proteins using
monomeric avidin■ Use immobilized AviTag-fusion proteinsfor high-throughput screening andprotein-protein interaction studies
using surface plasmon resonance■ Visualize Avi-Tag-fusion proteins using
avidin or streptavidin conjugates withwestern blots and MHC-tetramers forstaining and sorting T cells

Biotin ligase protein in vitro or
Expressed biotin ligase in vivo

Figure 4. Expression and biotinylation of proteins with
AviTag5

Multi-Purpose
HaloTag® Technology
The HaloTag® protein is a geneteically engineered
derivative of a dehalogenase. It efficiently forms
a covalent bond with various synthetic HaloTag ®
ligands. The 34 kDa monomeric protein can be fusedat either the N- or C-terminus to proteins of interest
and enables tagged proteins to be labeled withfluorophores for both in vitro and in vivo imaging orwith affinity agents for purification.

Applications■ Multicolor cell imaging with either live or fixedcells■ Facilitating protein purification■ Enhancing protein expression and solubility

Internal Ribosome Entry Site (IRES)
The IRES technology allows the coordinated coexpression of two genes using the same promoter
in a single vector. Virtually any combination ofgenes is possible. For example, you can monitor thedelivery of one gene by using a fluorescent reporterof a second gene or express a protein of interestand simultaneously biotinylate it with biotin ligase
expressed on the same vector.

Figure 5. Covalent and specific bindingof a variety of synthetic reporter andaffinity ligands to HaloTag ® proteins
allows detection, affinity-binding or solidphase fixation of proteins of interest.

Applications■ Monitor gene delivery efficiency■ Monitor protein modification■ In vivo biotinylation■ Stable transfection

OmicsLinkTM Anti-Tag Antibodies
GeneCopoeia offers OmicsLink anti-tag
antibodies to meet customers’ needs of working
with tagged fusion proteins expressed using
the OmicsLink ORF cDNA clones.
■ Anti-GFP antibody■ Anti-mCherry antibody■ Anti-GST antibody■ Anti-D* antibody■ Anti-Myc antibody■ Anti-HA antibody■ Anti-His antibody

Figure 6. Biocistronic expression of two genes with IRES.

*Also known as flag tag

6

Shuttle Clones
Depending on the application and budget, GeneCopoeia offers cDNA clones that meet every researcher’s
protein expression needs.

ORFEXPRESS Gateway ® PLUS
ORF cDNA Shuttle Clones
ORFEXPRESS Gateway® PLUS shuttle clones
offer both recombination cloning and multiple
cloning sites (MCS) for traditional cloning.
■ The presence of attL1 and attL2 sites allow
rapid and simple transfer of ORF inserts
into any Invitrogen Gateway ® destination
expression vector■ Flanking the ORFs, MCS make these clones
compatible with traditional cloning systems
that utilize classical restriction enzyme
digestion and ligation cloning methods■ 25,000 human and 20,000 mouse genes arecovered■ Available with or without stop codons■ Ribosomal binding sites (Shine Dalgarno andKozak Sequence) for optimal translational
context

Figure 7. Gateway® PLUS shuttle ORF cDNA clones
can be used for both recombination as well as
traditional restriction digestion ligation cloning.

ORFeome Collaboration ORF
cDNA Clones

Full-length whole transcript
cDNA template clone collection

As an official member of the ORFeome Collaboration,
GeneCopoeia carries and sells ORFeome ORF cDNA
clones. These ORF clones have been generated by
the contribution from various research institutes
including Dana Farber Cancer Institute-Center for
Cancer Systems Biology, NCBI, WISI/HAVANA
group and IMAGE Consortium.

GeneCopoeia offers 16,000 human full-length
whole transcript cDNA template clones. Theseclones include the 5’ and 3’ UTRs and involve
tedious multiple cloning steps for obtaining an
expression-ready construct.

■ 8000 human ORFeome collaboration clones■ Gateway® entry vectors for transferring into
any type of Gateway® compatible destination
expression vector■ No multiple cloning sites for traditional cloning

To save time and publish faster, GeneCopoeia
recommends using OmicsLink ExpressionReady ORF cDNA clones.
■ Ready-to-transfect and ready-to-express■ Overcome difficulties in expression,
production and purification with choice of100+ expression vectors■ Fully sequence-verified

7

Gene Search
Simple and multiple search options■ Keywords■ BLAST■ Gene or protein families and groups■ Gene and disease associations■ Gene ontology classification■ Subcellular localization

Click tosearchproducts by

KeywordGene
ontologyGenefamilies

BLAST
Subcellular
localization

Gene & diseaseassociations

Browse gene families and disease associations
Based on GeneCopoeia’s proprietary literature mining algorithm, over 10,000 genes have been associatedwith major disease categories. Browsing these associations using the browsing function on the GeneCopoeia
website search page makes finding genes of interest straightforward and convenient.

Gene Families

ORF
cDNAs

Disease Families

ORF
cDNAs

Cytokines

315

Cardiovascular diseases

1596

Cytokine receptors

152

Congenital anomalies and genetic
diseases

3978

Druggable target genes

6245

Digestive system diseases

864

G protein-coupled receptors

718

1886

Histone modification enzymes

38

Diseases of the blood and bloodforming organs

Histone proteins

66

1784

Ion channels

463

Endocrine, metabolic and nutritiondiseases

Membrane-bound proteins

2138

Immunologic diseases

3644

Nuclear hormone receptors

105

Infectious diseases

3536

Mental disorders

1805

Proteases

625

Musculoskeletal system diseases

946

Protein kinases

933

Neoplasms

8950

Protein phosphatases

293

Nervous system and sense organs

2404

Surface antigens (CD)263

Respiratory system diseases

565

Transcription factors

1096

Urologic and genital diseases

1304

Organelle markers

77

Skin and connective tissue diseases

866

Other kinases

201

Symptoms and general pathology

2022

8

Custom Services
Custom gene synthesis,
mutagenesis, cloning

Custom lentiviral particle
production

GeneCopoeia offers de novo gene synthesis
services for any gene that is not currently
available. GeneCopoeia can also customize
sequence composition, splicing variants andfunctional domains or fragments.

Lentiviral vectors are potent vehicles fordelivering genes into a wide range of cell types
including difficult-to-transfect and non-dividing
cells. However, producing, concentrating andtitrating lentiviral particles are time consuming
and require experience to achieve high titers
and consistent results.

Guaranteeing 100% sequence accuracy,
GeneCopoeia scientists use codon optimization
techniques to ensure high levels of expression inhost cells. Synthesized genes can be deliveredin any of more than 100 different vector types at
no additional custom service fee.

GeneCopoeia’s experienced scientific expertise
consistently produces high quality and hightiter crude or purified viral particles to meet yourresearch need in an efficient and cost-effective
way.

Gene Synthsis,
Mutagenesis,
Cloning

Lentivirus
Production

Protein
Production

Antibody
Production

9

Protein production

Antibody production

GeneCopoeia’s protein production facility uses
a range of host-cell and cell-free expression
systems including E. coli, insect, yeast, CHO andmammalian cells as well as wheat germ cell-freesystems. Protein expression, solubility and yield
are significantly increased by using a variety of
unique fusion tags that are not available in themarket.

GeneCopoeia custom services for poly- andmonoclonal antibody production specializes inusing recombinant proteins and different animal
species for unmatched low costs and shortdelivery times.

Each case is unique. Contact us today to
discuss how GeneCopoeia can help youwith your specific research needs.

Related Products
Coupled with GeneCopoeia cDNA clone collections, supporting and related products are alsoavailable to meet the protein expression and functional genomics research needs.
Category

Product

Description

Lentifect™ Lentivirus Production
Services

High-titer crude or purified lentiviral particles produced byexperts and ready-for-transduction

Lenti-Pac™ Lentiviral Packaging
Kits• Optimized lentiviral packaging plasmid mix• eGFP control clone• EndoFectin™ Lenti, a new transfection reagent developed
to work with lentiviral-based constructs• TiterBoost™, a reagent that further increases titers by 5-10
fold

Lenti-Pac™ 293Ta Lentiviral
Packaging Cell LineFor high-titer lentiviral production using Lenti-Pac™ lentiviral
packaging kits

qPCR Kits andPrimers

All-in-One™ qPCR Kits andPrimers• Universal reaction conditions for all qPCR primers• Validated gene-specific primers for human, mouse and rat

Transfection
Reagents

EndoFectin™ Lenti
EndoFectin™ CHO
EndoFectin™ Plus
EndoFectin™ MAX

Fully optimized and validated for specific cell types

Anti-Tag
Antibodies

OmicsLink™ Antitag Antibodies

Monoclonal mouse IgG anti-tag antibodies that bind to 6xHis-,
GFP-, mCherry-, GST-, D*-, HA-, or Myc-tagged fusion protein

shRNA
Expression
ClonesOmicsLink™ shRNA Expression
Clones• Genome-wide coverage of human, mouse and rat• Four shRNA constructs per target gene
• Guaranteed knockdown effect of 70% determined by qRTPCR

Pre-miRNA
Expression
ClonesmiExpress™ Precursor miRNA
Expression Clones• Fully sequenced and optimized for high expression andmaturation of miRNA inside cells• Full coverage of human, mouse and rat miRNA in miRBase
database

miRNA
Inhibitor
Expression
ClonesmiArrest™ miRNA Inhibitor
Expression Clones• Superior potency, long-lasting inhibition and extremely low
cell toxicity• Full coverage of human, mouse and rat miRNA in miRBase
database

miRNA 3’UTR
TargetExpression
ClonesmiTarget™ miRNA 3’UTR Target
Expression Clones• Genome-wide coverage of human, mouse and rat miRNA
3’UTR target sequences• Dual luciferase reporters or dual reporters in a single vector

Lentiviral
System*Also known as flag tag

10Selected Expression Vectors
Mammalian Expression Vectors with CMV Promoter and Neomycin
Selection
Tag

Protease Site

Mammalian
Mammalian

Selection
MarkerNeomycin
Neomycin

N-His
C-His+IRES-eGFP

N/A
N/A

CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV

Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian

Neomycin
Hygromycin
Neomycin
Puromycin
Neomycin
Neomycin
Neomycin
Neomycin
Neomycin
Neomycin
Neomycin
Neomycin

C-His
N/A
N/A
N/A
N-eGFP
C-eGFP
N-eYFP
C-eYFP
N-eCFP
C-eCFP
N-GST
N-Avi

N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
EK
N/A

pReceiver-M48

CMV

Mammalian

Neomycin

N-Avi+IRES-Biotin ligase

N/A

pReceiver-M62

CMV

Mammalian

Neomycin

C-Avi+IRES-Biotin ligase

N/A

pReceiver-M17

CMV

Mammalian

Neomycin

C-Avi

N/A

pReceiver-M06
pReceiver-M07
pReceiver-M08

CMV
CMV
CMV

Mammalian
Mammalian
Mammalian

Neomycin
Neomycin
Neomycin

N-3xHA
C-3xHA
C-3xHA-His

N/A
N/A
N/A

pReceiver-M43
pReceiver-M45
pReceiver-M09
pReceiver-M10
pReceiver-M72
pReceiver-M11
pReceiver-M12
pReceiver-M13
pReceiver-M46
pReceiver-M14

CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV

Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian
Mammalian

Neomycin
Neomycin
Neomycin
Neomycin
Neomycin
Neomycin
Neomycin
Neomycin
Neomycin
Neomycin

N-Myc
C-3xHA+IRES2-eGFP
C-Myc
C-Myc-His
C-Myc+IRES-eGFP
N-FlagN-3XFlag
C-Flag
C-Flag+IRES-eGFP
C-3XFlag

N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A

pReceiver-M49

CMV

Mammalian

Neomycin

N-HaloTagpReceiver-M50

CMV

Mammalian

Neomycin

C-HaloTag

Tev proteasa
Tev proteasa

pReceiver-M55
pReceiver-M56
pReceiver-M61

CMV
CMV
CMV

Mammalian
Mammalian
Mammalian

Neomycin
Neomycin
Neomycin

N-mCherry
C-mCherry
IRES2-eGFP

N/A
N/A
N/A

VectorPromoter

Host CellpReceiver-M01
pReceiver-M51

CMV
CMV

pReceiver-M77
pReceiver-M67
pReceiver-M02
pReceiver-M68
pReceiver-M29
pReceiver-M03
pReceiver-M15
pReceiver-M16
pReceiver-M32
pReceiver-M33
pReceiver-M04
pReceiver-M05

Insect Expression Vector

11

VectorPromoter

Host Cell

Selection Marker

Tag

Protease SitepReceiver-I01

AcMNPV polyhedrin

Insect cell

N/A

N-His

Tev

Selected Expression Vectors
Lentiviral Expression Vectors with CMV Promoter for Stem,Primary andOtherDifficult-to-Transfect Cells
VectorPromoter

Host Cell

Selection
MarkerTag

Protease SitepReceiver-Lv01
pReceiver-Lv105
pReceiver-Lv81
pReceiver-Lv36
pReceiver-Lv80
pReceiver-Lv76
pReceiver-Lv78
pReceiver-Lv79
pReceiver-Lv40
pReceiver-Lv21
pReceiver-Lv02
pReceiver-Lv52
pReceiver-Lv06
pReceiver-Lv23
pReceiver-Lv03
pReceiver-Lv53
pReceiver-Lv07
pReceiver-Lv19
pReceiver-Lv04
pReceiver-Lv08
pReceiver-Lv20
pReceiver-Lv05
pReceiver-Lv09
pReceiver-Lv34
pReceiver-Lv61
pReceiver-Lv62
pReceiver-Lv68
pReceiver-Lv35
pReceiver-Lv26
pReceiver-Lv10
pReceiver-Lv25
pReceiver-Lv17
pReceiver-Lv18
pReceiver-Lv46
pReceiver-Lv77
pReceiver-Lv48
pReceiver-Lv75
pReceiver-Lv45
pReceiver-Lv47
pReceiver-Lv70
pReceiver-Lv64
pReceiver-Lv65
pReceiver-Lv71
pReceiver-Lv72
pReceiver-Lv73
pReceiver-Lv41
pReceiver-Lv67
pReceiver-Lv66

CMV
CMV
CMV
CMV
CMV
PGK
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
PGK
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
CMV
EF1α†
CMV
CMV

Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cell
Stem/primary cellNo
Puromycin
N/A
No
N/A
N/A
N/A
N/A
Neomycin
NeomycinNo
N/A
Neomycin
NeomycinNo
N/A
Neomycin
NeomycinNo
Neomycin
NeomycinNo
Neomycin
NeomycinNo
Neomycin
N/A
No
Neomycin
Neomycin
Neomycin
Neomycin
Neomycin
N/A
N/A
N/A
N/A
N/A
Neomycin
N/A
Neomycin
Neomycin
Puromycin
Neomycin
N/A
Neomycin
Puromycin
Hygromycin

N/A
N/A
IRES2-eGFP+IRES-luciferase
IRES2-mCherry
IRES2-eGFP
IRES2-eCFP
IRES2-eYFP
IRES-Neomycin
N/A
C-3xHA
C-3xHA+IRES2-eGFP
C-3xHA
N-Flag
C-Flag
C-Flag+IRES2-eGFP
C-FlagN-eGFP
C-eGFP
C-eGFP
N-eYFP
C-eYFP
C-eYFP
N-eCFP
C-eCFP
C-eCFP
C-Avi + IRES-Biotin ligase
N-Avi + IRES-Biotin ligase
N-Avi
C-Avi
N-Myc
C-Myc
C-Myc-His
C-Myc+IRES-luciferase
C-Myc+ IRES2-eGFP
C-Myc+ IRES2-eYFP
C-Myc+ IRES2-mCherry
C-Myc+ IRES2-eCFP
C-Myc+IRES-Neomycin
C-Myc+ IRES2-eGFP
N-HaloTag
C-HaloTag
N-mCherry
C-mCherry
C-His+ IRES-eGFP
N/A
N/A
N/A

N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
Tev protease*
Tev protease*
N/A
N/A
N/A
N/A
N/A
N/A

*Tev protease site†
EF1α promoter
12Selected Expression Vectors
Yeast Expression Vectors
VectorPromoter

Host Cell

Selection
MarkerTag

Protease SitepReceiver-Y01

GAL1

S. cerevisiae

N/A

C-His

N/A

pReceiver-YAD

pADH

Yeast

N/A

GAL4AD

N/A

pReceiver-YBD

pADH

Yeast

N/A

GAL4DB

N/A

Bacterial Expression Vectors
VectorPromoter

Host Cell

Selection Marker

Tag

Protease SitepReceiver-B01

T7

E. Coli

N/A

N-His

N/A

pReceiver-B31

T7

E. Coli

N/A

C-His

N/A

pReceiver-B02

T7

E. Coli

N/A

N/A

N/A

pReceiver-B03

T7

E. Coli

N/A

N-GST

Tev

pReceiver-B04

T7

E. Coli

N/A

N-GST

EK

pReceiver-B05

Tac

E. Coli

N/A

N-GST

Tev

pReceiver-B06

Tac

E. Coli

N/A

N-GST

EK

pReceiver-B07

Tac

E. Coli

N/A

N-MBP

Tev

pReceiver-B08

Tac

E. Coli

N/A

N-MBP

EK

pReceiver-B09

T7

E. Coli

N/A

N-Avi

N/A

pReceiver-B10

Tac

E. Coli

N/A

N-FlagN/ApReceiver-B11

Tac

E. Coli

N/A

N-His

N/A

pReceiver-B12

Tac

E. Coli

N/A

HisSUMOSUMO proteasepReceiver-B13

T7

E. Coli

N/A

HisSUMOSUMO protease

Wheat Germ Cell-Free Expression Vectors

13

VectorPromoter

Host Cell

Selection Marker

Tag

Protease SitepReceiver-WG02

T7

cell free

N/A

N-His

Factor Xa

pReceiver-WG03

T7

cell free

N/A

N-HisSUMO

CoolCutter™

pReceiver-WG04

T7

cell free

N/A

N-AviSUMO

CoolCutter™

pReceiver-WG05

T7

cell free

N/A

N-HisAviSUMO

CoolCutter™

pReceiver-WG09

T7

cell free

N/A

HisGST

TEV

pReceiver-WG16

T7cell free

N/A

N/A

N/A

pReceiver-WG31

T7cell free

N/A

N-HisSUMOAvi

CoolCutter™

pReceiver-WG33

T7cell free

N/A

N-TrxHisSUMO

CoolCutter™

Selected Publications
Save time • Increase productivity • Publish Faster
111 Hernandez CP, et al. Pegylated arginase I: a potential therapeutic approach in T-ALL. Blood. 2010 Jun
24;115(25):5214-21.
222 Kennedy MJ, et al. Syntaxin-4 defines a domain for activity-dependent exocytosis in dendritic spines. Cell.
2010 Apr 30;141(3):524-35.
333 Gandhi R, et al. Activation of the aryl hydrocarbon receptor induces human type 1 regulatory T cell-likeand Foxp3(+) regulatory T cells. Nat Immunol. 2010 Sep;11(9):846-53.
444 Clemen CS, et al. Strumpellin is a novel valosin-containing protein binding partner linking hereditary
spastic paraplegia to protein aggregation diseases. Brain. 2010 Oct;133(10):2920-41.
555 Lee K, et al. Flexible use of nuclear import pathways by HIV-1. Cell Host Microbe. 2010 Mar 18;7(3):22133.
666 Or YY, et al. Identification of a novel 12p13.3 amplicon in nasopharyngeal carcinoma. J Pathol. 2010
Jan;220(1):97-107.
777 Xia M, et al. p21 does not protect cancer cells from apoptosis induced by nongenotoxic p53 activation.
Oncogene. 2011 Jan 20;30(3):346-55.
888 Fornuskova D, et al. Novel insights into the assembly and function of human nuclear-encoded cytochromec oxidase subunits 4, 5a, 6a, 7a and 7b. Biochem J. 2010 May 27;428(3):363-74.
999 Truitt L, et al. The EphB6 receptor cooperates with c-Cbl to regulate the behavior of breast cancer cells.
Cancer Res. 2010 Feb 1;70(3):1141-53.
1111 Uysal-Onganer P, et al. Wnt-11 promotes neuroendocrine-like differentiation, survival and migration of
prostate cancer cells. Mol Cancer. 2010 Mar 10;9:55.
1111 Gustavsson E, et al. SOX11 expression correlates to promoter methylation and regulates tumor growth inhematopoietic malignancies. Mol Cancer. 2010 Jul 12;9:187.
1111 Delisle BP, et al. Small GTPase determinants for the Golgi processing and plasmalemmal expression ofhuman ether-a-go-go related (hERG) K+ channels. J Biol Chem. 2009 Jan 30;284(5):2844-53.
1111 Taguchi Y, et al. Specific biarsenical labeling of cell surface proteins allows fluorescent- and biotin-tagging
of amyloid precursor protein and prion proteins. Mol Biol Cell. 2009 Jan;20(1):233-44.
1111 Métayé T, et al. Immunohistochemical detection, regulation and antiproliferative function of G-proteincoupled receptor kinase 2 in thyroid carcinomas. J Endocrinol. 2008 Jul;198(1):101-10.
1111 Muik M, et al. Dynamic coupling of the putative coiled-coil domain of ORAI1 with STIM1 mediates ORAI1
channel activation. J Biol Chem. 2008 Mar 21;283(12):8014-22.
1111 Ruvolo VR, et al. PKR regulates B56(alpha)-mediated BCL2 phosphatase activity in acute lymphoblastic
leukemia-derived REH cells. J Biol Chem. 2008 Dec 19;283(51):35474-85.
1111 Blish KR, et al. A human bone morphogenetic protein antagonist is down-regulated in renal cancer. Mol
Biol Cell. 2008 Feb;19(2):457-64.
1111 Tada M, et al. Down-regulation of hedgehog-interacting protein through genetic and epigenetic alterations
in human hepatocellular carcinoma. Clin Cancer Res. 2008 Jun 15;14(12):3768-76.
1111 Wilson TJ, et al. Cathepsin G enhances mammary tumor-induced osteolysis by generating soluble
receptor activator of nuclear factor-kappaB ligand. Cancer Res. 2008 Jul 15;68(14):5803-11.
2222 Whipple RA, et al. Vimentin filaments support extension of tubulin-based microtentacles in detachedbreast tumor cells. Cancer Res. 2008 Jul 15;68(14):5678-88.
2222 Navarro-Borelly L, et al. STIM1-Orai1 interactions and Orai1 conformational changes revealed by live-cell
FRET microscopy. J Physiol. 2008 Nov 15;586(Pt 22):5383-401.

14