The 108th Annual Meeting of the American Academy of Periodontology
in collaboration with the Japanese Academy of Clinical Periodontology,
and the Japanese Society of Periodontology.

Abstracts of JACP/JSP Poster Session

October 27-30, 2022
Phoenix Convention Center, Phoenix, Ariz, USA

The Japanese Academy of Clinical Periodontology
The Japanese Society of Periodontology

第108回アメリカ歯周病学会共催
日本臨床歯周病学会・日本歯周病学会
2022年大会
The 108th Annual Meeting of the American Academy of Periodontology
in collaboration with the Japanese Academy of Clinical
Periodontology, the Japanese Society of Periodontology.

JACP/JSP ポスターセッション抄録集

一般演題（基礎研究，臨床研究），症例報告，歯科衛生演題

Abstracts of JACP/JSP Poster Session
General (Basic Research, Clinical Research), Case Report, Dental Hygiene

会期：2022年10月27日～10月30日
会場：フェニックス・コンベンションセンター

October 27-30, 2022
Phoenix Convention Center, Phoenix, Ariz, USA

book JSPJACPポスターセッション2022.indb

12022/10/07

14:12:02

第108回アメリカ歯周病学会共催
日本臨床歯周病学会・日本歯周病学会 2022年大会
JACP/JSP ポスター発表協賛企業

アース製薬株式会社
相田化学工業株式会社
医歯薬出版株式会社
花王株式会社
科研製薬株式会社
クインテッセンス出版株式会社
サンデンタル株式会社
株式会社ゼロメディカル
タカラベルモント株式会社
デンタルプロ株式会社
ネオス・ジャパン株式会社
白水貿易株式会社
株式会社メディアート
株式会社モリムラbook JSPJACPポスターセッション2022.indb（50音順）

2

2022/10/07

14:12:02

General（Basic Research）

book JSPJACPポスターセッション2022.indb

12022/10/07

14:12:02

book JSPJACPポスターセッション2022.indb

22022/10/07

14:12:02

1

GB-01

TNF-α regulates human odontogenic ameloblast-associated protein gene transcription in
gingival epithelial cells
Yorimasa Ogata＊1,2, Arisa Yamaguchi1, Mizuho Yamazaki-Takai1,2, Hideki Takai1,2,
Yohei Nakayama1,2, Yuto Tsuruya1

1

Department of Periodontology, Nihon University School of Dentistry at Matsudo, Matsudo, Japan, 2ResearchInstitute of Oral Science, Nihon University School of Dentistry at Matsudo, Japan

Background and objective: Odontogenic ameloblast-associated protein（ODAM）is produced by junction epithelium（JE）
and maturation stage ameloblasts. Function of ODAM is thought to be involved in the attachment of JE and teeth. To
investigate transcriptional regulation of human ODAM gene in inﬂamed gingiva, we have analyzed the eﬀects of TNF-α on
the expression of ODAM gene in Ca9-22 gingival epithelial cells.
Materials and Methods: Total RNAs and proteins were extracted from Ca9-22 cells after stimulation by TNF-α（10 ng/ml）,
and mRNA and protein levels of ODAM were analyzed by qPCR and Western blot. Luciferase（LUC）analyses were
performed using LUC constructs inserted in various lengths of ODAM gene promoter. Gel shift and chromatin
immunoprecipitation（ChIP）assays were carried out.
Results: TNF-α increased ODAM mRNA and protein levels at 3 to 24 h. TNF-α induced LUC activities of the ODAM genepromoter constructs, and the activities were inhibited by PKA, tyrosine kinase, MEK1/2, PI3-kinase and NF-κB inhibitors.
Gel shift and ChIP assays revealed that TNF-α increased CCAAT/enhancer binding protein（C/EBP）β and Yin Yang1（YY1）binding to three kinds of C/EBPs and YY1 elements.
Conclusion: These results demonstrate that TNF-α stimulates ODAM gene transcription via C/EBPs and YY1 elements in
the human ODAM gene promoter.

GB-02

Osteoclastogenic and anti-osteoclastogenic cytokines induced by dental calculus
Megumi Mae＊, Mohammad Ibtehaz Alam, Yasunori Yamashita, Yukio Ozaki,
Masayuki Oohira, Atsutoshi Yoshimura

Department of Periodontology and Endodontology, Nagasaki University Graduate School of Biomedical Sciences
Background and objective: Dental calculus（DC）is frequently found in periodontitis patients and composed of bacterial
components and calcium phosphate crystals. We have shown that the crystal components of DC promote IL-1β production
via NLRP3 inﬂammasome in macrophages. Because IL-1β is known to promote osteoclastogenesis, we investigated theeﬀects of cytokines produced by mouse macrophages stimulated with DC on osteoclastogenesis.
Materials and Methods: DC from periodontitis patients was pulverized, treated with 10％ sodium hypochlorite, andextensively washed with distilled water. Wild-type（WT）and NLRP3-deﬁcient mouse macrophages were stimulated with
DC and the culture supernatants were harvested. M-CSF and RANKL-primed mouse bone marrow-derived macrophages（BMMs）and RAW-D cells were incubated with these supernatants. Following TRAP staining, the number of multinuclear
TRAP positive cells was counted. The concentrations of IL-1β, IL-18, and IL-10 in the culture supernatants were measured
by ELISA. To analyze the effects of these cytokines, osteoclasts were generated from BMMs and RAW-D cells in thepresence or absence of recombinant（r）IL-1β, IL-18, or IL-10.
Results: The culture supernatant from WT mouse macrophages promoted osteoclastogenesis of BMMs but inhibited that of
RAW-D cells. WT mouse macrophages stimulated with DC produced IL-1β, IL-18, and IL-10 dose-dependently whereas
NLRP3-deficient mouse macrophages produced IL-10, but not IL-1β and IL-18, in response to DC. rIL-1β promoted
osteoclastogenesis of RANKL-primed BMMs and RAW-D cells, but rIL-18 and rIL-10 inhibited their osteoclastogenesis.
Conclusion: DC may promote alveolar bone resorption via IL-1β induction in periodontitis patients, but suppress resorption
via IL-18 and IL-10 induction in some circumstances.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

12022/10/07

14:12:02

2

GB-03

Role of Piezo1 in mechanical stress-mediated bone resorption with periodontitis
Mabuki Uchinuma＊1, Yoshimasa Taketani1, Yuichiro Sugiyama1,2,
Junya Ueda1, Makiko Ishii1, Kitetsu Shin1

1

Division of Periodontology, Department of Oral Biology and Tissue Engineering, Meikai University School of
Dentistry, 2Meikai University Post-Doctoral Institute Saitama Dental Clinic

Background and objective: Excessive occlusal force with periodontitis results in rapid alveolar bone resorption. However,
the molecular mechanism underlying the interaction between inﬂammation and mechanical stress has yet to be completely
elucidated. To clarify the mechanism, we focused on Piezo1, which is a mechanosensitive ion channel expressed on
osteoblasts. The changes in the RANKL/OPG ratio in osteoblast-like MC3T3-E1 cells cultured with lipopolysaccharide
from Porphyromonas gingivalis（P.g-LPS）in which Piezo1 was activated by mechanical stress.
Materials and Methods: MC3T3-E1 cells were cultured in osteogenic medium with P.g-LPS. After 3 days of culture, shear
stress induced by an orbital shaker was applied intermittently as mechanical stress to the cells at 200 rpm for 20 min per
cycle. The stress was loaded for ﬁve cycles at 10-min intervals. Piezo1 function was subsequently inhibited by the additionof the Piezo1 antagonist GsMTx4 or by knockdown via Piezo1 siRNA transfection. After loading, total RNA was isolated
from the cells, and real-time PCR was performed to assess the mRNA expression levels of Piezo1, RANKL, and OPG.
Results: In cells cultured with P.g-LPS, no signiﬁcant change was seen in the Piezo1 mRNA expression level, whereas theRANKL/OPG ratio signiﬁcantly increased after loading when compared to no loading. The increase in the RANKL/OPG
ratio was suppressed by the addition of GsMTx4 and by Piezo1 knockdown.
Conclusion: These results suggest that Piezo1 is involved in the mechanical stress-induced increase of the RANKL/OPG
ratio in MC3T3-E1 cells cultured with P.g-LPS.

GB-04

Combined eﬀects of FGF-2+DBBM on periodontal healing of poorly-contained defects
Tasuku Murakami＊1, Naoki Miyata1, Daisuke Matsugami1, Takahiro
Bizenjima2, Kentaro Imamura1, Fumi Seshima1, Atsushi Saito1,3

1

Department of periodontology, Tokyo Dental College, 2 Chiba Dental Center, Tokyo Dental College, 3 Oral Health
Science Center, Tokyo Dental College

Aim: In our previous study, the use of FGF-2 with DBBM was similarly effective as FGF-2 alone in the healing ofexperimental periodontal defects in rats. It was suggested that the combined use of FGF-2 and DBBM enhances healing via
promotion of cell proliferation, angiogenesis, and osteogenic diﬀerentiation. Combined use of signaling molecules and bone
substitutes is clinically recommended for the treatment of poorly-contained defects. The purpose of this study is toinvestigate the eﬀects of the combined use of FGF-2 and DBBM on the periodontal healing of poorly-contained defects in
rats.
Materials and Methods: Poorly-contained（2-wall）periodontal defects were created mesially to the maxillary ﬁrst molars
in Wistar rats. Defects were filled with FGF-2, DBBM, FGF-2+DBBM, or left unfilled. Histological examinations andmicrocomputed tomography were used to evaluate healing at 4 weeks postoperatively. In vitro, the positive VEGF
expression of rat bone marrow mesenchymal stem cells（BMSCs）after 72 h of seeding on DBBM with/without FGF-2
was assessed by confocal laser scanning microscopy（CLSM）.
Results: Histologically, greater bone formation was observed in FGF-2 and FGF-2+DBBM groups than in Unfilled or
DBBM groups. Bone volume fraction was signiﬁcantly greater in FGF-2+DBBM groups than that in Unﬁlled group. In
vitro, CLSM images revealed that the positive staining for VEGF in BMSCs was more frequently observed in FGF2+DBBM group compared with that in DBBM group.
Conclusions: It was suggested that DBBM acted as a scaﬀold, spacemaker, and carrier of FGF-2, and thereby accelerates
healing of poorly-contained defects in FGF-2+DBBM group.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

22022/10/07

14:12:02

3

GB-05

Endothelial insulin resistance contributes to the exacerbation of diabetes-related periodontitis
Tatsuro Zeze＊, Takanori Shinjo, Kohei Sato, Mio Imagawa, Yuki Nishimura,
Misaki Iwashita, Takao Fukuda, Terukazu Sanui, Fusanori Nishimura
Department of Periodontology, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University

Background and objective: Local insulin resistance is suggested to contribute to the exacerbation of periodontitis under
diabetes. However, it remains unclear how periodontal insulin resistance is involved in the pathogenesis of diabetes-related
periodontitis. In this study, we investigated the pathogenic role of insulin resistance on vascular endothelial cell function thatmediates the recruitment of inﬂammatory immune cells.
Materials and Methods: Using murine vascular endothelial cell line（TKD2）, the inhibitory eﬀect of insulin（100nM）on
E.coli LPS and TNFα（10ng/ml, respectively）-induced cell adhesion molecules（CAMs）expression and cellular adhesion
with human leukocyte cell line（THP-1）were assessed. Then, potential regulatory signaling pathway of insulin on CAMs
expression was investigated. Furthermore, using vascular endothelial cell-speciﬁc insulin receptor knockout（KO）mice,
7-0 silk ligature-induced alveolar bone loss and insulin signaling in mice gingiva were compared.
Results: Western blot analyses showed LPS and TNFα-induced VCAM-1 expression was significantly suppressed byinsulin pretreatment via the PI3K-Akt-FoxO1 pathway. Subsequently, insulin signiﬁcantly downregulated cellular adhesionof LPS and TNFα-treated TKD2 with THP-1. High（25mM）glucose treatment for 48 hours significantly inhibited
insulin-mediated activation of PI3K-Akt-FoxO1 pathway and abolished its regulatory eﬀect on VCAM1 expression. KO
mice showed signiﬁcantly less insulin action in the gingiva and exacerbated alveolar bone loss compared to WT mice.
Conclusion: These results suggest that insulin resistance in the vascular endothelial cells may promote the recruitment ofinﬂammatory immune cells in the gingiva by disturbing the regulatory eﬀect on VCAM-1 expression by insulin, resulting in
the exacerbation of periodontal tissue destruction.

GB-06

miR-1260b inhibits periodontal bone loss by targeting ATF6β

Chikako Hayashi＊, Kentaro Kawakami, Masaaki Toyoda, Yukari Watanabe, Yuki Nakao, Karen
Yotsumoto, Hiroaki Yamato, Takanori Shinjo, Terukazu Sanui, Takao Fukuda, Fusanori Nishimura
Department of Periodontology Division of Oral Rehabilitation, Faculty of Dental science, Kyushu University
Background and objective: We recently demonstrated that TNF-α stimulation enhanced the expression of miR-1260b in
human gingival MSCs（hGMSCs）-derived exosomes and these exosomes successfully inhibited periodontal bone loss inmice model（Nakao Y, et al., Acta Biomater, 2022）. It is also reported that ER stress-related genes are up-regulated inperiodontal lesion（Yamada H, et al. J Periodontal Res., 2002）, and database analysis suggested that miR-1260b could be apossible regulator of ER stress by targeting ATF6β. In this study, we, therefore, investigated the therapeutic eﬀect of miR1260b in periodontal lesion by targeting ATF6β.
Materials and Methods: Human periodontal ligament cells（hPDLCs）were transfected with miR-1260b to conﬁrm theinhibition of ATF6β. The effect of miR-1260b on inflammatory bone loss were validated in mouse ligature-induced
periodontitis model（Kyushu University, #A21-131-2）. The expression of ATF6β in mice gingiva was observed byimmunohistochemistry and periodontal bone loss was evaluated by microCT analysis. PBMCs and RAW-D cells were
cultured with supernatants of hPDLCs transfected by ATF6β siRNA and the number of TRAP positive cells were counted.
Results: Transfection of miR-1260b mimic inhibited ATF6β expression and knock-down of ATF6β decreased the expressionof RANKL in hPDLCs. Increased expression of ATF6β was observed in the ligated periodontal lesion, and the local injectionof miR-1260b mimic decreased periodontal bone resorption in mice model. Supernatants of hPDLCs transfected by ATF6β
siRNA inhibited osteoclast formation.
Conclusion: miR-1260b inhibited periodontal bone loss by targeting ER stress. Thus, miR-1260b therapy can be a novel
strategy against periodontal bone loss.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

32022/10/07

14:12:03

4

GB-07

The potential mechanism of CTLA-4-Ig-mediated osteoclast diﬀerentiation
Saki Nakane-Koyachi＊1, Kentaro Imamura1, Tasuku Murakami1, Rio Hisanaga1,
Kazuyuki Ishihara2,3, Atsushi Saito1,2

1

Department of Periodontology, Tokyo Dental College, Tokyo, Japan, 2Oral Health Science Center, Tokyo Dental
College, Tokyo, Japan, 3Department of Microbiology, Tokyo Dental College, Tokyo, Japan

Background and objective: Cytotoxic T lymphocyte-associated antigen-4（CTLA-4）is one of the immune checkpoint
receptors expressed on the surface of T cells. In our previous study, we showed that the administration of CTLA-4-Ig
reduced the bone resorption and the number of osteoclasts in a mouse periodontitis model. The expression of protein
phosphatase 2A（PP2A）was increased by CTLA-4-Ig. However, the mechanism remains unclear. The purpose of thisstudy is to investigate the potential mechanism by which CTLA-4-Ig regulates osteoclast diﬀerentiation.
Materials and Methods: RANKL and CTLA-4-Ig were added to RAW264.7 cells. The eﬀect of CTLA-4-Ig on nuclear
factor kappa B（NF-κB）activation was evaluated by western blot analysis. Gene expression of PP2A in RAW264.7 cells
was suppressed by siRNA transfection. Cell viability was evaluated by WST-8 assay. The expression of osteoclastdifferentiation markers（Cathepsin K, Trap）was assessed by qRT-PCR, and the number of osteoclast-like cells wasevaluated by TRAP staining in transfected cells.
Results: NF-κB phosphorylation was suppressed by the treatment of CTLA-4-Ig. The siRNA transfection had no signiﬁcanteﬀect on cell viability. In the PP2A siRNA transfected group, CTLA-4-Ig induced no signiﬁcant suppression of Cathepsin K
and Trap expressions. The number of osteoclast-like cells tended to increase in the PP2A siRNA transfected group comparedto the control siRNA transfected group.
Conclusion: These data suggested that regulation of the NF-κB pathway via modulation of PP2A expression is one of themechanisms by which CTLA-4-Ig inhibits osteoclast diﬀerentiation.

GB-08

Eﬀects of senolytic drugs on aged mice
Naoto Koyama＊1,2, Shinya Kato1, Yasuhiro Tai2, Ryusuke Sako2, Suguru Nakamura2,
Nanae Dewake2, Yuki Ozaki2, Nobuo Yoshinari1,2

1

Department of Oral Health Promotion, Graduate School of Oral Medicine, Matsumoto Dental University,
Department of Operative Dentistry, Endodontology and Periodontology, School of Dentistry, Matsumoto Dental
University

2

Background and objective: Senescent cells induce chronic inﬂammation in various organs and are a reported cause of aging.
Controlling chronic inﬂammation by eliminating senescent cells may lead to the development of comprehensive therapies
for periodontal disease and atherosclerosis, which are based on chronic inﬂammation. In this study, we examined the eﬀects
of senescence inhibition by senolytic drug treatment in an animal model of aged mice.
Materials and Methods: Twenty-ﬁve 69-week-old wild-type C57BL6 mice and eight 89-week-old ApoE−/− mice, amodel of genetic hyperlipidemia, were used. These mice were further divided into a treatment group（12 C57BL6 and 3
ApoE−/− mice）and an untreated control group（13 C57BL6 mice and 5 ApoE−/− mice）. The treatment group
received the senolytic drug for 2.5 months, followed by euthanization and removal of periodontal tissue and the aorta.
Periodontal tissues were examined for alveolar bone resorption using micro-computed tomography imaging. Maxillarybones were stained with β-galactosidase, a marker of cellular senescence. Aortas were stained with Zudan IV stain tomeasure the fat deposition rate.
Results: Between-group comparisons of alveolar bone resorption showed that age-related resorption was suppressed in thetreatment group. Among the ApoE−/− mice, the fat deposition rate in the treatment group was decreased compared withthat in the control group.
Conclusion: The inhibition of alveolar bone resorption and the reduction in aortic fat deposition in senolytic drug-treatedApoE−/− mice may serve as a model for studying the relationship between periodontal disease, atherosclerosis and aging,
all of which involve chronic inﬂammation.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

42022/10/07

14:12:03

5

GB-09

P.gingivalis gingipains potentially aﬀects MUC5AC expression in respiratory epithelial cells
Yukihiro Karahashi＊1,2, Kenichi Imai2, Syuichi Sato1
1

Department of Periodontology, Nihon University School of Dentistry, 2Department of Microbiology and
Immunology, Nihon University School of Dentistry

Background and objective: It has long been established that periodontal diseases contribute to a particular set of pulmonary
diseases, such as pneumonia and COPD. However, a causal relationship between periodontopathic bacteria and the onset ofpneumonia and COPD has not yet been established. Under normal conditions, mucus clearance occurs in a host as a ﬁrst lineof defense against airborne pathogens and pollutants, however, under pulmonary disease conditions like pneumonia and
COPD, mucus hypersecretion occurs, potentially contributing to disease pathology and mortality. In addition, of thenumerous mucins found in airway epithelial cells, MUC5AC and MUC5B comprise approximately 90％ of overall mucin
content and more importantly, MUC5AC expression and protein levels are elevated in pneumonia and COPD patients.
Therefore, Porphyromonas gingivalis（P.g.）may influence both MUC5AC expression and protein levels in airway
epithelial cells, potentially contributing to the aggravation of pneumonia and COPD. Here, the remit of this study was toestablish whether P.g. virulence factors aﬀected MUC5AC in immortalized and primary bronchial cells.
Results: MUC5AC gene expression and protein levels are aﬀected by P.g. culture supernatant, but not by lipopolysaccharide
or FimA ﬁmbriae. Cells treated with either P.g. single（Kgp or Rgp）or double（Kgp/Rgp）mutants had altered levels of
MUC5AC gene expression and protein levels, and MUC5AC staining of double mutant-treated mouse lung cells showedthat MUC5AC protein levels were unaﬀected.
Conclusion: P.g. gingipains may be the primary virulence factor that inﬂuences both MUC5AC gene expression and protein
levels, potentially contributing to pneumonia and COPD aggravation.

GB-10

Characterization of the genes associated with stress response in Treponema denticola

Keiko Yamashita＊1, Yurie Kitamura1, Rio Hisanaga1,2, Yuichiro Kikuchi2,3, Eitoyo Kokubu2,3,
Takashi Namba4, Kazuyuki Ishihara2,3, Atsushi Saito1,2
1

Department of Periodontology, 2Oral Health Science Center, 3Department of Microbiology, Tokyo Dental College,
Tokyo, Japan, 4Private Practice, Tokyo, Japan

Background and objective: The stress-response mechanism in Treponema denticola, one of the major periodontal pathogens,has yet to be elucidated. Previously, we found that the expression of a potential transcriptional regulator was signiﬁcantly
increased in the T. denticola mutant deﬁcient in major outer sheath protein（Msp）. Because loss of Msp may cause stress onouter sheath of T. denticola, we hypothesized that the gene might be involved in the stress response. Therefore, we focusedon an operon including the potential transcriptional regulator. The aim of this study is to elucidate the part of stress-response
mechanism in T. denticola, through analyzing the function of this operon.
Materials and Methods: We constructed two mutant strains deﬁcient in each gene located in the operon from T. denticola
ATCC35405（wild-type strain）. The gene-expression proﬁles of the mutants were analyzed by DNA microarray and qRTPCR. Based on the results of the genetic analyses, we investigated some phenotypic changes of the mutants.
Results: In the genetic analyses, we observed the diﬀerential expression of the genes involved in the motility of T. denticola.
The mutant deﬁcient in the potential transcriptional regulator showed no change in growth, while that of the mutant deﬁcientin the downstream gene was attenuated. Furthermore, the motility of both mutant strains was significantly decreased
compared with that of wild-type strain.
Conclusion: The target genes in the operon might be involved in the response against the stress on outer sheath of T.
denticola, through regulating the motility of this bacterium.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

52022/10/07

14:12:03

6

GB-11

Eﬀect of Porphyromonas gingivalis on gut in relation to aging
Sarita Giri＊1, Osamu Uehara2, Ayuko Takada3, Durga Paudel4, Tetsuro Morikawa5,
Toshiya Arakawa3, Toshiyuki Nagasawa6, Yoshihiro Abiko5, Yasushi Furuichi1

1

Division of Periodontology and Endodontology, 2Division of Disease Control and Molecular Epidemiology,
Division of Biochemistry, 4Advanced Research Promotion Center, 5Division of Oral Medicine and Pathology,
6
Division of Advanced Clinical Education, Health Sciences University of Hokkaido, Japan

3

Background and objective: Periodontal tissue destruction is more frequently observed in the elderly. The translocation of
periodontal pathogens, including P. gingivalis to the gut has been shown to alter the gut microbiome. However, the eﬀect of
P. gingivalis on the gut environment in relation to aging has not been demonstrated. We hypothesize that P. gingivalis has amore detrimental eﬀect on the gut environment with increased age.
Materials and Methods: C57BL/6J mice aged 4 weeks（young）or 76 weeks（old）were divided into four groups:
control-young, control-old, P. gingivalis-administered young, and P. gingivalis-administered old. The P. gingivalis groupwere orally administered with P. gingivalis thrice weekly for 5 weeks. After 30 days of the last P. gingivalis administration,
the gut microbiome was studied using 16S rRNA sequencing. The mRNA expression of intestinal junctional barrier
molecules and the levels of the inﬂammatory cytokines IL-1β and TNF-α in the serum was evaluated.
Result: Significant differences in the gut microbiomes between the groups, in terms of taxonomic abundance, bacterial
diversity, and predicted metagenome function was observed. A significant reduction in the alpha diversity and in theabundance of beneﬁcial bacteria, such as Akkermensia and Clostrodiaceae, in the P. gingivalis-administered old mice wasobserved. The mRNA levels of Claudin-1 and Claudin-2 in the intestine were significantly elevated in P. gingivalisadministered old mice, as were the serum levels of IL-1β and TNF-α.
Conclusion: The eﬀect of P. gingivalis on the gut environment is more pronounced in old mice than in young mice.

GB-12

Local delivery of Backpack-bound M2 macrophages -Cellular immunotherapy against
periodontitisMayuka Nakajima＊, Koichi Tabeta

Division of Periodontology, Department of Oral Biological Science, Niigata University Graduate School of Medical
and Dental Sciences
Background and objective: Proinﬂammatory M1 macrophages are the predominant type in periodontitis. It was demonstrated
that inducing anti-inflammatory M2 macrophages prevented the disease progression in a mouse periodontitis model.
Cellular immunotherapy, transfer immune cells, is expected to have a more direct and specific effect than using drugs;
however, adopted immune cells can alter their function in undesirable directions in response to environmental conditions.
Herein, we developed micro particles referred to as a “backpack（BP）” that adhered to macrophage helping the cells tomaintain M2 phenotype in the disease site and tested its eﬃcacy against experimental periodontitis.
Materials and Methods: BPs are fabricated with spin-coating method. IL-4（200fg/BP）loaded BPs were attached to bonemarrow–derived macrophages（BMDMs, C57BL/6）by incubation on dishes. Polarization of BP-bound MBDMs to M2
was observed by flow cytometry. The BP-bound M2 cells（BP-M2）, BPs or M2 cells were injected to the periodontal
region that induced by ligation and inoculation of Porphyromonas gingivalis W83, and therapeutic eﬃcacy was assessed byalveolar bone loss measurement. Additionally, localization and phenotype of the macrophages in the disease sites wereobserved by immunohistology.
Results: BPs polarized BMDMs to M2 in vitro. Injected BP-M2 cells were localized in the disease sits over 5days and highratio of M2 to total macrophages was observed only in BP-M2 injection group. Alveolar bone loss was signiﬁcantly reducedin the BP-M2 group when compared with the other groups.
Conclusion: Transferred BP-bound macrophages localized and maintained M2 phenotype in periodontal region, and thereby
prevent the disease progression.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

62022/10/07

14:12:03

7

GB-13

Determining the best approach for sequencing analysis of oral microbiota
Takahiko Nagai＊1, Takahiko Shiba1,2, Keiji Komatsu3, Nemoto Takashi1,
Ryota Kobayashi1, Yasuo Takeuchi1, Takanori Iwata1

1

Department of Periodontology, Tokyo medical and dental university, 2Department of Oral Medicine, Infection, andImmunity, Harvard School of Dental Medicine, 3Department of Lifetime Oral Health Care Sciences, Tokyo Medical
and Dental University
Background and objective: 16S rRNA gene amplicon sequencing analysis is widely used to investigate the diversity andcomplexity of microbial communities in oral diseases. However, the selected ampliﬁcation regions may distort the accurate
data assessment. This study aimed to evaluate the bias of 16S rRNA gene amplicon analysis using primers targeting diﬀerent
hypervariable regions.
Materials and Methods: Bacterial DNA samples were prepared from mock1 community comprising of 15 bacteria fromvarious environments, mock2 community comprising of 6 major oral bacteria, and samples from the dental calculus of 5
patients. DNA samples were ampliﬁed with 9 primers and sequenced with 300 bp paired ends on Illumina Miseq platform,
and the data was analyzed using QIIME2 with databases；SILVA, Greengenes, and Human Oral Microbiome Databasedatabases（HOMD）.
Results: In the genus-level analysis of mock1 community, the similarity of relative bacterial abundance ratio to thetheoretical value was highest in V1-V2 region with SILVA. In the analysis of mock2 community, the highest levels were
evident in V4-V5 region with HOMD at the genus-level and V1-V2 region with HOMD at the species-level. In thespecies-level analysis of the dental calculus sample, the number of detected bacteria was highest in V1-V2 region with
HOMD. The relative bacterial abundance was more inﬂuenced by the samples compared to the primer type.
Conclusion: The use of primers targeted V1-V2 region, 300 bp paired ends sequencing, and HOMD database might show
the most reliable result for 16S rRNA gene ampliﬁcation sequencing analysis of the oral microbiome.

GB-14

Fabrication of stem cell-based scaﬀold-free bone-like 3D structures

Masaaki Toyoda＊1, Shunichi Kajioka2, Ryota Fujimoto3, Chikako Hayashi1, Kentarou Kawakami1,
Yukari Watanabe1, Yuki Nakao1, Karen Yotumoto1, Terukazu Sanui1, Takao Fukuda1,
Koichi Nakayama3, Fusanori Nishimura1
1

Department of Periodontology, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University,
Department of Pharmacy, Fukuoka College of Pharmaceutical Sciences, International University of Health andWelfare, 3Center for Regenerative Medicine, Saga University School of Medicine

2

Background and objective: Although periodontal tissue regenerative therapy has been widely used to recover alveolar boneabsorbed by periodontal disease, its indications are limited. In this study, we aimed to establish a new strategy to regenerate
alveolar bone by creating cellular structures using mesenchymal stem cells with bone diﬀerentiation ability by using a bio3D printer by "Kenzan Method" that can create scaﬀold-free cellular structures.
Materials and Methods: Cell clusters（spheroids）were prepared from human gingival stem cells（hGMSCs）using celllow attachment surface 96-well plates. Cluster size measurements were performed and expression of stem cells（CD73）
and apoptosis（Cleaved Caspase-3）markers were conﬁrmed by ﬂuorescent immunostaining. After 4 weeks of osteogenic
diﬀerentiation, frozen sections were prepared for evaluation of calciﬁcation by Alizarin Red and Von Kossa staining. μCT
analysis was also performed to measure the bone mineral density（BMD）.
Results: 4.0×10^4 cells/well were seeded, and spheroid with a diameter of 500-600 μm was successfully created to enablestructure formation. Immunofluorescent staining revealed the expressions of stem cell markers, and a small amount of
apoptosis markers without affecting the growth of spheroid were observed. Four weeks after osteogenic differentiation,
calciﬁcation of the bio-3D printed spheroids was conﬁrmed by Alizarin Red staining and Von Cossa staining. μCT analysis
further revealed that the BMD of the calciﬁed structure was almost identical to that of trabecular bone.
Conclusion: Using a bio-3D printer, scaﬀold-free bone-like structures were successfully fabricated from gingival-derived
stem cells.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

72022/10/07

14:12:03

book JSPJACPポスターセッション2022.indb

82022/10/07

14:12:03

General（Clinical Research）

book JSPJACPポスターセッション2022.indb

12022/10/07

14:12:03

book JSPJACPポスターセッション2022.indb

22022/10/07

14:12:03

9

GC-01

Evaluation of oral health-related quality of life following free gingival graft procedure
Yoshimasa Taketani＊1, Takafumi Suzuki1, Jun Takenoya1,2, Yusuke Yamane1,
Haruki Kodama1, Hideharu Otsuka1, Joichiro Hayashi1, Kitetsu Shin1

1

Division of Periodontology, Department of Oral Biology and Tissue Engineering, Meikai University School of
Dentistry, 2Meikai University Post-Doctoral Institute Saitama Dental Clinic

Background and objective: The free gingival graft（FGG）procedure is a commonly used approach in clinical practice forincreasing vestibular depth and width of the attached gingiva. FGG is considered more invasive and a heavier burden on
patients compared to other periodontal surgical procedures because of its open wounds. The aim of this study was toquantitatively examine patient burden following FGG through questionnaires that evaluated the oral health-related qualityof life（OHQoL）and pain.
Materials and Methods: The study evaluated 21 patients who underwent FGG or open ﬂap debridement（OFD）on theirmandibular molars. The questionnaires used the General Oral Health Assessment Index（GOHAI）for OHQoL, visual
analogue scale（VAS）for pain. Subjects were also evaluated using three subscales of GOHAI（physical function,
psychosocial function, and pain and discomfort）.
Results: There was a signiﬁcant decrease in the GOHAI score at day 3 after FGG as compared to baseline. Physical andpsychosocial function scores at day 3 were signiﬁcantly lower in FGG versus OFD. Furthermore, the VAS score increased
signiﬁcantly at day 3 in both groups and no diﬀerence between the groups during the evaluation period.
Conclusion: These results suggest that FGG causes a temporary decline in OHQoL due to physical and psychosocial
function eﬀects. This study demonstrated that FGG requires postoperative follow-up for not only pain but also physical andpsychosocial function.

GC-02

Interfacial ﬁndings of implants removed because of peri-implantitis
Nanae Dewake＊1, Naoto Koyama1, 2, Ichiro Kawahara3, Akio Ueda4, Akane Miyakuni1,
Shinya Kato1, 2, Yuki Ozaki 1, Nobuo Yoshinari 1, 2

1

Department of Operative Dentistry, Endodontology and Periodontology, School of Dentistry, Matsumoto Dental
University, 2Department of Oral Health Promotion, Graduate School of Oral Medicine, Matsumoto Dental
University, 3Center of Health Care, Matsumoto Dental University Hospital, 4Division of Oral Implantology,
Matsumoto Dental University Hospital

Background and objective: The pathogenesis and course of peri-implantitis（PI）have not been fully clariﬁed. Furthermore,
few reports have described the implant surfaces aﬀected by PI. We herein report our histological observations of the surfacesof implants that were removed after 30 years because of PI.
Materials and Methods: Two implants were cut in the remaining osseointegration area using a carbide bar and extracted. The
implant samples were immersed and fixed in a 10％ neutral formalin solution. The sample of the first molar area wasencapsulated in resin, and a polished specimen was prepared for observation under an optical microscope. The sample of thesecond bicuspid area was dehydrated and freeze-dried, and specimens for scanning electron microscopy（SEM）were prepared.
Results: The surface of the extracted implant showed black discolored areas with no attachment of tissue other thaninflammatory tissue, including a small piece of bone, under SEM examination. In the polished specimen, inflammatory
tissue and exfoliated bone tissue were observed on the implant interface. SEM observation also showed numerous foci ofbacterial adherence, mainly filamentous bacteria. Sulfide reactions were observed in areas that appeared black
microscopically. In addition, the degenerated bone tissue showed many fatty droplets, which are seen in poorly metabolized
tissue.
Conclusion: The PI–implant interface was contaminated by sulﬁde adherence and bacterial infection, and degenerated bone
tissue was also observed. These ﬁndings, when considered in conjunction with clinical symptoms, may assist in the selectionof treatment for PI.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

92022/10/07

14:12:03

10

GC-03

Relationship between periodontal disease, diabetes mellitus, and liver diseaseToshiya Fujii＊, Norio Aoyama, Sayuri Kida, Kentaro Taniguchi, Tomomi Yata, Motohiro Komaki
Periodontology, Kanagawa Dental University
Background and objective: A bidirectional relationship between diabetes and periodontitis and a higher incidence of liverdisease in periodontitis patients have been shown. The proportion of patients with diabetes who progress from nonalcoholic
fatty liver disease to liver cancer was twice as high as that of healthy individuals. However, there are still few reports thatcomprehensively analyzed the relationship between diabetes, liver function, and periodontal disease. The purpose of thisstudy was to clarify the relationship of those diseases, focusing on diabetes index（HbA1c）, periodontal index（PISA）, andliver function markers.
Materials and Methods: Subjects were recruited from 2018 to 2021 at the Medical and Dental Collaboration Centre,
Kanagawa Dental University Hospital. HbA1c was measured to assess diabetes. PISA was calculated from periodontal
clinical examination. AST, ALT and γ-GTP were measured as liver function markers. The correlation between diabetes,
periodontal disease and liver function markers was examined. The study was approved by the Kanagawa Dental University
Ethical Review Committee（No. 553）.
Results: A total of 277 subjects（182 women and 95 men）with a mean age of 67 years were included in the study. Higher
proportion of high PISA patients was found in the high HbA1c group compared to the low HbA1c group. Only AST was anincreased marker among liver function indicators in the high HbA1c group. AST and γ-GTP were also increased in the high
PISA group.
Conclusion: The results of the present study conﬁrmed the association between diabetes and periodontitis and showed theirassociation with liver function.

GC-04

Relationship between Meal Sequence, Nutrients and Number of Teeth
Sayuri Kida＊1, Norio Aoyama1, Taizo Iwane2, Toshiya Fujii1, Kentaro Taniguchi1,
Tomomi Yata1, Motohiro Komaki1
1

Periodontology, Kanagawa Dental University, 2Kanagawa University of Human Services

Background and objective: Oral diseases are strongly linked with systemic disorders. Eating behavior such as meal sequenceis important for maintaining good general health. Poor oral condition could cause malnutrition, however, the eﬀect of oral
condition on food intake has not been clariﬁed. The purpose of this study was to assess the relationship between awarenessof meal sequence and nutrients in the various oral condition of Japanese people.
Materials and Methods: The subjects were recruited at the Medical and Dental Collaboration Center in Kanagawa Dental
University Hospital from 2018 to 2021. Clinical examinations such as body composition analysis, periodontal measurement,
and assessment of chewing ability were performed and data of meal sequence was collected. Brief-type self-administrated
diet history questionnaire（BDHQ）was used to assess nutrients. The Ethics Committee of Kanagawa Dental University
approved the protocol of the present study（No. 801）.
Results: 238participants with mean age of 67.5 were enrolled in this study. 79 people had an eating behavior, in which theytake meal sequence of vegetables, meat or ﬁsh and carbohydrate. On the other hand, 61people had completely no awarenessof the meal sequence. People who had the awareness of the meal sequence obtained proper nutrients in dietary fiber,
calcium, and vitamin C compared with those who had no awareness. While people with many teeth obtained sufficientnutrients according to the awareness of food intake, people with less than 20 teeth obtained inadequate nutrients even if they
had the awareness of meal sequence.
Conclusion: People with a few teeth could not get enough nutrition even if they are aware of meal sequence.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

102022/10/07

14:12:03

11

GC-05

Impaired oral functions in subjects with insuﬃcient recognition of umami
Norio Aoyama＊, Tomomi Yata, Sayuri Kida, Taizo Iwane, Toshiya Fujii,
Kentaro Taniguchi, Motohiro Komaki
Periodontology, Kanagawa Dental University, Kanagawa University of Human Services

Background and objective: Oral condition is closely related with systemic health. Oral function directly affects dietaryintake, and tasty food intake is an essential function to live; however, scientiﬁc data about the association between taste
decline and oral health is limited. Thus, the aim of this study was to assess the relationship sensitivity of umami, one of ﬁve
tastes, and oral condition.
Materials and Methods: Subjects were recruited from January 2018 to June 2021 in Kanagawa Dental University Hospital.
Taste inspection with a whole mouth method was performed using tap water, 1％ sweet water, 0.3％ salty water, and low（0.03％）and high（0.1％）concentration of umami water. Lifestyle habits like miss a meal were asked and clinical tests
for oral functions were performed. The study was approved by the Kanagawa Dental University Ethical Review Committee（No. 801）.
Results: There was no difference of age according to recognition of umami. Those who could not recognize low
concentration of umami water had a tendency of decreased residual teeth. Recognition of umami was associated withoccluding area, oral diadochokinesis, and salt recognition. Those who regularly missed a meal had impaired umami
recognition.
Conclusion: This study suggests that diminished umami recognition was related with reduction of occluding surface areaand lowered tongue and lip motor function. Impaired oral functions were found in the subjects with insuﬃcient recognitionof tastes.

GC-07

Eﬀectiveness of antimicrobial photodynamic therapy in the periodontal maintenance phase
Yasunori Yamashita＊, Megumi Mae, Masayuki Oohira, Yukio Ozaki, Atsutoshi Yoshimura

Department of Periodontology and Endodontology, Nagasaki University Graduate School of Biomedical Sciences
Background and objective: Antimicrobial photodynamic therapy（a-PDT）combined with scaling root planing（SRP）is
more effective at improving periodontal status than SRP alone. However, the effectiveness of a-PDT combined withirrigation for patients undergoing periodontal maintenance has not been clariﬁed. This study aimed to evaluate the eﬃcacy
of a-PDT in the periodontal maintenance phase compared to irrigation.
Materials and Methods: This study was approved by the Nagasaki University Hospital Certiﬁed Review Board（CRB19013）. Thirty patients who visited Nagasaki University Hospital for periodontal maintenance from 2019 to 2020 were
included in this study. Patients who had multiple sites with bleeding-on-probing（BOP）and periodontal probing depths（PPD）of 4–6 mm in the maintenance phase were treated with a split-mouth design. These sites were randomly assigned to
one of two groups: the a-PDT group or the irrigation group. In the irrigation group, the periodontal pockets were simply
irrigated using an ultrasonic scaler. In the a-PDT group, the periodontal pockets were irrigated using an ultrasonic scaler,
injected with light-sensitive toluidine blue（FotoSan®agent, CMS Dental ApS, Denmark）, irradiated with an irradiator, andthen once again irrigated using an ultrasonic scaler. After 7 days, the safety and eﬃcacy of a-PDT were assessed.
Results: PPD and BOP signiﬁcantly improved in the a-PDT group, but not in the irrigation group. No adverse events wereobserved in any patients.
Conclusion: a-PDT combined with irrigation was eﬀective in improving PPD and BOP in patients undergoing maintenance.
a-PDT may be useful as a non-invasive treatment in the maintenance phase.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

112022/10/07

14:12:03

book JSPJACPポスターセッション2022.indb

122022/10/07

14:12:03

Case Reportbook JSPJACPポスターセッション2022.indb

12022/10/07

14:12:03

book JSPJACPポスターセッション2022.indb

22022/10/07

14:12:03

13

R-01

Periodontal Regenerative Therapy for Severe Bone Defect in Maxillary Canines
Yuichiro Ihara＊1, Taneaki Nakagawa2

1

Ihara Dental Clinic, Tokyo, 2Department of Dentistry and Oral Surgery, Keio University School of Medicine

Background: There remains controversy about whether teeth involved with severe bone loss should be saved or extracted.
Patientsʼ needs are diverse, but many patients wish to save their teeth. Here we present two cases in which maxillary canines
accompanied by severe bone defects.
Case: Case1）A 51-year-old female visited in February 2015. Her chief complaint was to treat periodontitis. #23 had aprobing depth（PD）of 10mm at the palatal site and the presence of bleeding. Tooth mobility was grade Ⅱ.
Case2）A 55-year-old female visited in March 2019. Her chief complaint was gingival swelling. #23 had an over 10mmPD at the palatal site and the presence of bleeding. Tooth mobility was grade Ⅱ.
Clinical Procedures and Outcomes: After initial therapy, periodontal regeneration using a combination of enamel matrix
derivative, deproteinized bovine bone mineral, and collagen membrane was performed. Five mm of PD and the presence of
bleeding remained at a re-evaluation 1 year after surgery in case 1, thus additional regenerative therapy was performed.
Periodontal tissues in both cases 1 and 2 improved and have been clinically stable for 6 years, and 2 years and 6 months
post-surgery, respectively.
Conclusion: The maxillary canine, which has significant role in occlusal relations, seemed to be difficult to achieve
periodontal regeneration since there is a severe bone defect. Although it might be one option to extract a hopeless canine,
optimal periodontal regenerative therapy could achieve saving the teeth, which led to higher patient satisfaction and theimprovement of QOL.

R-02

A case series of periodontal regenerative therapy using a non-incised papillae surgicalapproach
Kotaro Tsuchida＊
Tsuchida dental clinic

Background and objective: Periodontal regenerative therapy is an eﬀective treatment to improve bone loss, inhibit further
progression of periodontal disease, and contribute to the oral health of patients. In recent years, various ﬂap designs havebeen devised with better results. In this report, we present three cases of deep vertical bone defects treated with periodontal
regenerative therapy using the non-incised papillae surgical approach（NIPSA）to preserve the interdental papillae.
Cases: Patients were 43, 56, and 76 years old（all female）, no underlying disease, one smoker and two nonsmokers, allwith Stage IV Grade C severe periodontitis, and vertical bone defects greater than 3 mm.
Results: OHI, scaling, and SRP under local anesthesia were performed. No antimicrobial agents were used. Periodontal
regenerative therapy was performed for vertical bone defects using a dental microscope and NIPSA. After debridement,
EMD was applied and a bovine bone（Bio-oss）was placed in the bone defect. Sutures were placed with 6-0
polypropylene and removed after 1 week. PPD decreased and attachment level improved. PPD decreased and attachmentlevel improved. The radiographic ﬁndings showed improvement of the bone defect morphology.
Conclusion: If the bone defect can be approached from the buccal side, NIPSA is eﬀective in preserving the interdental
papillae adjacent to the defect.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

132022/10/07

14:12:03

14

R-03

Long-term success for peri-implantitis and peri-implant mucositis through stepwise
approaches
Eiji Ichimaru＊1, Kiwako Ogawa1, Yasunori Yoshinaga2,3, Ryusuke Yamaguchi1
1

Shin-Tosu Periodontal & Implant Dentistry Center, Ichimaru Dental Oﬃce, KURANOUE, 2Section of
Periodontology, Department of Odontology, Fukuoka Dental College, 3Oral Medicine Research Center,
Fukuoka Dental College

Background: Peri-implant diseases are common inflammatory bacterial infections. Standard therapy has not yet been
established. This report presented the successful management of peri-implant mucositis and peri-implantitis throughstepwise approaches for more than eight years.
Case: A 62-year-old, systemically healthy, non-smoker, Japanese woman with generalized periodontitis（stage Ⅲ, gradeB）in March 2009. The patientʼs periodontitis was well controlled with regular periodontal therapy. Implants were placed at#31, #30, #28, #20, and #18 tooth sites. Mucositis around all the implants were overlooked in March 2010. Peri-implantitis
developed around #31, #30, and #18 implant sites.
Clinical Procedures and Outcomes: Peri-implant mucositis was actively treated through non-surgical therapy and periimplantitis through non-surgical therapy followed by additional surgical therapy, since November 2013, consecutively:
OHIs, repetitive and meticulous nonsurgical debridement of implants, modiﬁcation of prosthesis to improve oral hygiene,
resective surgery through decontamination with implantoplasty, air-abrasion using β-TCP powder, and a mesio-apically
positioned flap; supportive peri-implant therapy with repetitive debridement of the implant surfaces every one to twomonths. The peri-implant inﬂammation and bone resorption resolved seven years after the surgical therapy. A small amountof exudate dominant with neutrophils was occasionally observed at the #18 implant site.
Conclusion: These consecutive therapies were eﬀective in achieving long-term resolution of both peri-implant mucositis
and peri-implantitis. The practical goal for therapy of peri-implantitis should be cessation of progressive bone loss withmild mucosal inflammation. Complete elimination of inflammation can be that for peri-implant mucositis. It should be
important to detect and initiate peri-implant therapy in early peri-implant mucositis.
R-04

Multiple implant-supported occlusal reconstruction case utilizing digital and analog
technology
Shogo Ando＊
Namikidori Dental Clinic

Background: Implant overdenture is a treatment option for cases of edentulous or multiple missing teeth. However, themethod requires numerous diagnostic procedures, particularly when the tooth in the intercuspal position is missing. Using
digital simulation, a treatment plan can be visualized and treatment duration can be shortened. Furthermore, patients canbetter understand their treatment goal beforehand. Here, we report about digitally simulated occlusal reconstruction that may
help make treatment procedures less complex and shorten treatment durations for edentulous patients.
Case: A 72-year-old woman complained of misfit in her two dentures. She had lost her only remaining tooth when
undergoing retention for maxillary denture. She was also dissatisﬁed with her previous mandibular denture. Because of hervomiting reﬂex, she asked for a palate-less denture.
Clinical Procedures and Outcomes: The patient was not particularly dissatisﬁed with the removable dentures; therefore, weplanned to ﬁt a maxillary palate-less overdenture and a mandibular implant-assisted removable partial denture. We started
the procedure with digital simulation based on the information of vertical dimension, occlusal plain, stable jaw position, andimplant position. After deciding the vertical dimension, the implant position and ﬁnal prosthesis were determined. After the
implant was placed exactly at the planned position less invasively, the frame was prepared using laser sintering based on theprosthesis design obtained through digital simulation, and the ﬁnal prosthesis was set successfully.
Conclusion: The use of digital simulation can visualize a treatment plan and is sufficient to obtain functional andaesthetically satisfactory results even for a occlusal collapse case.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

142022/10/07

14:12:03

15

R-05

A case of generalized severe chronic periodontitis with malocclusion and occlusal trauma
Nobuaki Imaeda＊
Imaeda Dental clinic

Background and objective: we report a case of severe chronic periodontitis within a dentition demonstrating secondary
occlusal trauma, which was improved by periodontal and orthodontic treatment.
Case: 51-year-old woman
housewife
First visit: January 9, 2015
Chief complaint: “The upper front teeth are mobile from several months ago. I feel swelling with blood coming out of mygums when brushing my teeth.” Opinion: More than PPD 4mm 71.4％, BOP75.0％, #11 Degree of mobility 2, #16、26
Degree of furcation lesion 3, ＃45 missing teeth, A stenotic dental arch with marked lingual tilt of the upper and lower
molars is observed.
Clinical Procedures and Outcomes: At the first visit, there was a concern of treatment cost, and corrective orthodontic
treatment was not desired. The treatment plan involved periodontal treatment only. As the basic periodontal therapy
progressed, the patient's awareness changed, and a modified treatment plan involving orthodontic treatment wasincorporated. This time by utilizing orthodontic treatment, the continuity of the dentition could be maintained and function
was favorable by keeping tooth extraction to a minimum. The patient was satisﬁed with this outcome.
Conclusion: Orthodontic treatment as a pretreatment for prosthetic treatment leads to preservation of enamel, pulp and teeth,
and ultimately has the potential to be minimally invasive when re-intervention is considered in future. Orthodontic treatment
is not suitable for all patients, but we believe that it will continue to be an eﬀective method for preserving many teeth. The
dentition of the patient remained stable, even after the conclusion of treatment due to the patientʼs improved healthawareness during initial periodontal treatment. We would like to continue reviewing the patientʼs long term prognoses in the
future.

R-06

Successful regenerative response of severe bone defect with cemental tear
Takayoshi Nagahara＊1, Katsuhiro Takeda2, Hideki Shiba2

1

Nippon Kokan Fukuyama Hospital, 2Department of Biological Endodontics, Graduate School of Biomedical and
Health Sciences, Hiroshima University

Background: Cemental tear involves complete or incomplete separation within the root surface along the cementodental
junction that can lead to loss of periodontal attachment. This case report showed the course of regenerative treatment foralveolar bone defects with cemental tear in a right lower central incisor（#25）.
Case: Cone-beam computed tomography（CBCT）and clinical examinations including pulp vital testing and pocket
probing depth showed a cemental tear with a severely labial alveolar bone defect, but no endodontic lesions in #25, which
had a sinus tract at the labial site, in a 75-year-old woman. This cemental tear was diagnosed as Class 3/Stage C based on
the classiﬁcation by Lee et al., 2021.
Clinical procedure and outcome: Clinical management of this case based on the treatment decision-making（Lee et al.,2021）included complete removal of the torn fragments and inflamed granulation tissues, and periodontal regenerative
therapy. Recombinant human ﬁbroblast growth factor（rhFGF-2）was used as a biologically regenerative molecule. The
treatment yielded the gain of 7-mm-clinical attachment level and reconstruction of the labial bone wall and proximal bonewalls 2 years after the surgery. In particular, the regenerated labial bone had a similar height to the lingual bone.
Histopathological examination of the removed cemental fragment and granulomatous tissue showed they both had bacterialcolonies.
Conclusion: Successful clinical and radiographic outcomes for severe periodontal tissue destruction with cemental tear wereachieved through the clinical management based on the new classiﬁcation and treatment decision-making by Lee et al.,2021.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

152022/10/07

14:12:03

16

R-07

Periodontal regenerative therapy using FGF-2: a 3-year follow-up case report
Fumi Seshima＊, Tomoko Hayashi, Atsushi Saito
Department of Periodontology, Tokyo Dental College

Background: In Japan, ﬁbroblast growth factor-2（FGF-2）has been clinically used as a periodontal regenerative therapy.
We report a 3-year follow-up of a patient with chronic periodontitis who received regenerative therapy using FGF-2.
Case: The patient was a 41-year-old woman who complained of mobile teeth in the anterior region. Initial examination
revealed 30.9％ of sites with a probing depth（PD）of ≥4 mm, full-mouth mean PD was 3.3 mm. The OʼLeary plaquecontrol record was 67.9％. Radiographic examination revealed angular bone resorption in #13,16,22,47. A clinical diagnosis
of generalized chronic periodontitis（Stage III, Grade C）was made.
Clinical Procedures and Outcomes: The patient underwent initial periodontal therapy including plaque control, scaling androot planing. After reevaluation, a series of surgical interventions were performed in #13,16,22,47. Regenerative therapy
using FGF-2（Regroth）was performed on the narrow intrabony defect of #13, and the combination therapy using FGF-2
and deproteinized bovine bone mineral（Bio-Oss）was performed on the wide and deep intrabony defects of #16,22,47.
Following reevaluation, then the patient was placed on supportive periodontal therapy. At 3 years postoperatively, #13
yielded clinical attachment level（CAL）gains of 4.0 mm and #16,22,47 yielded CAL gains of 5.2 ± 1.0 mm, respectively.
Distinct radiographic bone ﬁll was observed following the treatment with FGF-2. No sites showed PD of ≥4 mm, and themean full-mouth PD was reduced to 2.2 mm.
Conclusion: At 3 years postoperatively, the regenerative therapy using FGF-2 yielded improvements in clinical parameters
and radiographic bone ﬁll.

R-08

A Case Report of a Patient with Severe Chronic Periodontitis and Early Occlusal Breakdown
Treated with Periodontal Regeneration and Implant Therapy
Yoshihiro Kokura＊1, Naomi Miyake1, Mitsuhiro Iwata2
1

Kokura Dental Clinic, 2Sakura Dental Clinic

Background: This is a case report of a patient with severe chronic periodontitis and early occlusal breakdown that was
successfully treated with periodontal regeneration and implant therapy,
Case: A 68-year-old female visited our dental office in May 2014 with a chief complaint of a poor response to initialperiodontal treatment. The patient was a non-smoker and had been taking Fosamax®（alendronate sodium）for 10 years forosteoporosis. The Plaque Control Record（PCR）at the initial visit was 84％. Teeth #14, #15, and #18 had Class 3 mobility.
Teeth #3 and #18 had Class I and Class II furcation involvement. The occlusal relationship was Skeletal Class II with Angle
class II. Occlusal interferences were noted on teeth #15 and #18. The patient was diagnosed with Generalized Periodontitis
Stage III Grade C.
Clinical Procedures and Outcomes: Following initial treatment, a re-evaluation was performed. Fosamax was discontinued
prior to the surgical phase, and periodontal regeneration was performed for teeth #3, #18, and #19. A dental implant wasplaced at site #30. Teeth #14 and #15 were extracted and GBR was performed, followed by implant placement. Afterocclusal function was restored, a re-evaluation was completed and the patient was transitioned to SPT（supportive
periodontal therapy）. The PCR score continually decreased and the periodontal condition stabilized. The patient has been in
SPT for 6 years and the periodontal condition remains stable.
Conclusion: Further occlusal breakdown was prevented through periodontal regeneration and implant therapy, whichallowed for occlusal stabilization. It could be suggested that long-term stability will remain with continued SPT.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

162022/10/07

14:12:03

17

R-09

A case of periodontal orthodontic treatment for a patient with Stage III-Grade C extensive
chronic periodontitis with esthetic disturbanceDai, Kawanabe＊
Kawanabe Dental Clinic

Background and Objectives: Patients with severe periodontal disease may have pathological tooth movement, often resulting
in secondary occlusal trauma and esthetic disturbance. We report here a case for whom the combination of orthodontic
treatments not only met the esthetic requirements but also improved the periodontal disease.
Materials and Methods: A 62-year-old female patient came to the hospital with a chief complaint of halitosis. Rootbifurcation lesions in #24, #27, and #47, and vertical bone defects in #23, #24, #37, and #47 were observed by radiography.
In addition, #24 and #27 showed degree III and degree II agitations, respectively. After examination, a diagnosis of extensive
chronic periodontitis, Stage III-Grade C was made.
Results: The results of the reevaluation showed signiﬁcant improvement in BOP and PPD. Vertical bone defects in #23 and#37 were treated with periodontal tissue regeneration therapy using enamel matrix derivatives. The root bifurcation lesion in#27 was addressed with a trisection. After periodontal surgical treatment, orthodontic treatment was performed for thevertical bone defect in #47. Signiﬁcant improvement in periodontal pathology was achieved after the treatment.
Conclusion: In this case, orthodontic treatment was able to improve secondary occlusal trauma and esthetic disorders. Thissuggested that the use of orthodontic treatment for periodontal disease can improve periodontal disease as well as esthetic
disorders.

R-10

A case of Non-surgical periodontal therapy and orthodontic treatment for periodontitis, which
is presumed to have progressed bone resorption due to occlusal trauma
Kimitaka Kusunoki＊
Kusunoki Dental Clinic

Background: A patient with moderate generalized periodontitis was treated with basic periodontal therapy, but deep bone
defects remained in the mandibular anterior teeth. After improvement in bone levels, the patient underwent orthodontic andprosthetic treatment to restore full oral function.
Cases: Patient: A 61-year-old, female First visit: May 2018 Chief concern: swollen and painful gums in the lower leftcanine tooth region.
General history: Unremarkable, non-smoker
Dental history: She last had a restoration 2 years ago.
Diagnosis: Moderate to severe generalized periodontitis; Stage III Grade B
Treatment and results: After basic periodontal treatment（including removal of an ill-fitting prosthesis and root canaltreatment）, a deep bone defect remained in the mandibular anterior teeth region. The anterior teeth had a close occlusal
relationship with plexus and crossbite, whereas the molars had an improper occlusal relationship due in part to an ill-ﬁtting
prosthesis. The occlusal contact of the anterior teeth was eliminated by elevating the occlusal part of the molars with
provisional restorations, and re-SRP was performed. After improvement in the bone levels, teeth with poor prognosis wereextracted, and orthodontic treatment with multi-bracket was started. Eventually, the patient's occlusal condition improved
with prosthetic treatment. It is now 6 months since the completion of treatment and the patient's occlusion remains stable.
Conclusion: SRP after occlusal elevation of the molars showed early improvement in bone levels, but it cannot be clearlyshown whether it was due to SRP or the result of occlusal elevation. However, we believe that occlusal force is not
irrelevant. The buccal bone of the mandibular anterior teeth was originally quite thin, and orthodontic treatment caused
considerable gingival recession. However, the improvement of the dentition facilitated plaque control, and the condition of
the periodontal tissues has progressed favorably.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

172022/10/07

14:12:03

18

R-11

Eﬃcacy of re-entry surgery after regenerative therapy from long-term prognosis over 10 years
Tokuo Matsui＊
Kiwakai Dental Clinic

Background and Objective: With the clinical application of cytokines, such as EMD and FGF-2, regenerative therapy has
become widely applied for various bone defects. In addition, advances in materials and technology have made periodontal
regenerative therapy increasingly predictable, but It is diﬃcult to obtain 100% bone regeneration. In this poster, I present
eﬃcacy of re-entry surgery after regenerative therapy from long-term prognosis over 10 years.
Case:（1）A 54-year-old female. Signiﬁcant bone defects were observed in many teeth and regenerative therapies were
performed. I attempted to obtain as much physiological bone morphology as possible for the bone defects by regenerative
therapy. For residual bone defects after regenerative therapy osseous surgery was done to acquire physiological bonemorphology. As a result, long-term stability of the periodontal tissue has been maintained.Case :（2）62-year-old male. He
had moderate to severe periodontal disease in both maxilla and mandibula. The maxillary hopeless teeth were extracted, andapically positioned ﬂap（APF）was performed to eliminate periodontal pocket for the residual teeth. I treated the mandiblewith regenerative therapy for all teeth except those diagnosed as hopeless. After the regenerative therapy, to solve theproblem of the remaining periodontal tissues, osseous surgery was performed and the remaining periodontal pockets were
eliminated by free gingival grafting and adequate width of attached gingiva was obtained.
Result: In both cases, shallow gingival sulcus, physiological bone morphology, and adequate width of attached gingiva wereobtained and remained stable for a long period by re-entry surgeries.
Conclusion: Re-entry surgery after regenerative therapy is eﬀective in obtaining shallow gingival sulcus, physiological bonemorphology and attached gingiva necessary for stabilization of periodontal tissue.

R-12

The importance of a thorough periodontal examination before beginning orthodontic treatment
Satoru Morikawa＊, Kazuya Watanabe, Taneaki Nakagawa
Department of Dentistry and Oral Surgery, Keio University School of Medicine
Background: As the number of adult patients seeking orthodontic treatment has increased in recent years, orthodontists are
increasingly encountering patients with periodontal disease. Orthodontic treatment is not recommended for patients withperiodontal disease because it involves tooth movement caused by orthodontic forces compressing and resorbing theperiapical bone. There hasn't been a consensus on how to treat periodontitis after orthodontic treatment has begun.
Case: An orthodontist sent a 32-year-old woman to the Department of Dentistry and Oral Surgery after noticing acute signsof periodontitis in the maxillary molars, which necessitated a periodontal examination and treatment. Localized severe
periodontitis（Stage III grade B）was revealed in the maxillary left ﬁrst and second molars, as well as the mandibular rightsecond molar.
Clinical Procedures and Outcomes: After consulting with the orthodontist, the orthodontic treatment was put on hold toallow for periodontal treatment based on the results of the bacteriological examination. Oral sitaﬂoxacin was used to perform
Full-mouth disinfection. After therapy, periodontal and bacteriological exams revealed signs of bone regeneration andregression of localized periodontitis. The patient's orthodontic therapy was then continued, with excellent results.
Conclusion: Asymptomatic patients with localized severe periodontitis may pass the screening test before orthodontic
treatment; nevertheless, acute symptoms with bone resorption may occur during orthodontic treatment, causing treatment
suspension. On the other hand, a temporary orthodontic treatment interruption and rigorous periodontal intervention may aid
in recovery. All patients who need orthodontic treatment should see a periodontist rule out any periodontal problems that
could obstruct the orthodontic treatment.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

182022/10/07

14:12:03

19

R-13

A Case Report; Severe chronic periodontitis progression after treatment of intraosseous defects
Shoji Ishitani＊
Ishitani Dental Oﬃce

Background and objective: Since, intraosseous defects take many forms, their treatment requires detailed examinations withstrong site specificity awareness to determine defect morphology, preferably three-dimensionally. Furthermore, anappropriate treatment plan must then be established.
Materials and Methods:
Case: Patient: 48 years old, female, department store employer, smoker（10 cigarettes/day）First examination in May 1997. Chief complaint: crown of 26, dehiscence of each dowel core, subjective awareness of
clenching.
Diagnosis: Severe, chronic, extensive periodontitis, Stage III, Grade B
Results:
Treatment and results: 22: 24 extracted due to caries, so 23 was moved distally to alleviate crowding and achieve
spontaneous extrusion.
15: After orthodontic extrusion, periodontal surgery was performed for osteoplasty and ostectomy.
47: Periodontal tissue regeneration therapy was performed by autologous bone grafting.
Immediately after surgery, all PD values were within 3 mm, there was no bleeding, and X-radiography showed alveolarbone stability.
Conclusion: The treatment of intraosseous defects should be on a tooth-by-tooth basis, with awareness of site-speciﬁcity.
Furthermore, individual teeth should be treated ﬂexibly, based on an understanding of the intraosseous defect morphology.
Although it is important to ﬁll intraosseous defects, in clinical terms, it is more important to achieve a state with stable PD
values, absence of bleeding, and alveolar bone stability. To date, 22 years since treatment, completion the patient has lost
one tooth due to root fracture, but her prognosis remains generally favorable.
R-14

A case of periodontal regeneration therapy for cemental tear
Takao Kannari＊Aozora Dental Oﬃce

Background and objective: Cemental tear is defined as deep periodontal pockets and attachment loss with radiographperiapical lesion. There are two possible mechanisms for cemental tear: internal factors due to the structural weakness at thecementum-dentin interface and external factors due to stress such as dental trauma, occlusal trauma, and excessive occlusal
forces, but there is no clear evidence. This case is a case in which periodontal regenerative therapy was performed on a toothwith cemental tear during the regular maintenance.
Materials and Methods: The patient, a 49-year-female, had no periodontal disease and had been receiving regular
maintenance for 15 years. Abscess and deep periodontal pocket of the distal #13 were noted. There was no subgingival
calculus, pulp vital test was positive, CT images showed the distal and palatally of #13 with vertical bony defects, no part ofthe cementum fragment could be detected on the radiographs. Cemental tear was observed when the ﬂap was reﬂected andthe root surface was debrided. Periodontal regeneration therapy was performed with enamel matrix derivative（EMD）and.
bone graft（Cytrans Granules®）Results: One year after periodontal regeneration surgery, CT images showed improvement of the vertical bony defects. No
periodontal inﬂammation was observed and the patient's progress is good.
Conclusion: Cemental tear is rare, the exact aetiology, predisposing factors are not clear. Also, in many cases, preoperative
radiograph do not show a part of the cementum fragment. The pulpal status of the aﬀected tooth should be assessed, and thepossibility of preserving the tooth should be diagnosed based on the extent of the radiographic lesion and the location, sizeof the fragment, and the treatment plan should be considered.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

192022/10/07

14:12:03

book JSPJACPポスターセッション2022.indb

202022/10/07

14:12:03

Dental Hygienebook JSPJACPポスターセッション2022.indb

12022/10/07

14:12:03

book JSPJACPポスターセッション2022.indb

22022/10/07

14:12:03

21

H-01

A case report of periodontal treatment for a patient with dental phobia
Michiko Ogata＊, Shimizu Hiroyasu
Shimizu Dental Clinic, Tokyo, Japan

Background and objective: Dental phobia is a disorder characterized by fear of the dental clinic and dental treatment itself,
diﬃculty in treatment, and poor oral hygiene. In this report, we describe a case in which a patient with moderate periodontal
disease with dental phobia was treated with periodontal therapy and showed improvement.
Materials and Methods: The patient is a 43-year-old male. He had not seen a dentist in 35 years, and presenting symptoms
included gingival swelling, redness, and supragingival calculus. Clinical ﬁndings were PCR 100％ , BOP 100％ , periodontal
pockets ≥ 5 mm（10％）. The patient was a non-smoker. The diagnosis was localized moderate chronic periodontitis, andtreatment consisted of TBI, full mouth disinfection（FMD）, and extraction of wisdom teeth in the left and right upper andlower jaws. FMD and extraction were performed under intravenous sedation. After reevaluation, the patient's condition
stabilized, and followed by SPT for a year.
Results: After thorough oral hygiene instruction and non-surgical periodontal treatment, PCR was 15％, no periodontal
pockets larger than 5 mm, and BOP was 10％. One year has passed since the patient started SPT, and the condition has
remained stable. Although the patient was fearful of the treatment, he was relieved that the treatment was completed without
pain or unpleasant sounds by using intravenous sedation. The improvement in gingiva and halitosis were noticed and thepatient's lifestyle was also aﬀected.
Conclusion: This patient had moderately advanced periodontitis due to poor plaque control without long-term dental
intervention. Improvement of plaque control and application of pharmacotherapy, FMD and SPT, led to improvement of
periodontal tissues with maintenance of the quality of professional care. It is believed that patients with dental phobia need
to be given successful experiences with dental care and to change their own behavior in order to reduce risk factors forperiodontal disease.

H-02

Improving the treatment of severe periodoutitis by partial OHI
Yukiko Honda＊
Pieace Dental Clinic

Background: It is stated in the periodontal treatment guideline that basic periodontal treatment leads to stable condition
through establishing rapport with patients. This time, we report a new case in which oral hygiene was improved bynarrowing down OHI to the selected periodontally compromised tooth.
Case:A 53-year-old woman with no medical history presented to our clinic for malocclusion and dental checkup on July
2017. Chief complaint was that the tooth ﬁling on the right maxillary molar tooth was misaligned and could not be chewed.
Oral ﬁndings was that redness and swelling of the gingiva, periodontal pocket of 4 mm or more, and tooth mobility wereobserved on the whole.The BOP at the first visit was 63% and the PCR value was 52%. Dental X-ray images showedvertical bone resorption in the right ﬁrst molar and premolar, and left maxillary ﬁrst premolar.
Clinical Procedures and Results:
1. Response to the chief complaint and perform tooth extraction
2. Basic periodontal treatment
3. Reassessment
4. Oral function restoration treatment
5. Reassessment
6. Supportive periodontal therapy
Thorough OHI limited to the right mandibular ﬁrst molar led to improvement of the all self oral cleaning ability. The BOP
improve to 4% and the PCR value improved to 18% compared to value of the ﬁrst visit.
Conclusion: The establishment of selected plaque control improved self-care ability, and at the same time increasing
motivation for treatment and gaining participation in voluntary treatment, resulting in overall improvement in periodontal
tissue.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

212022/10/07

14:12:03

22

H-03

A case report of peri-implantitis treatment outcome for recall dropout patient
Kaori Teranishi＊
Osaka dental university hospital

Background: This is a case report of peri-implantitis treatment outcome as peri-implant bone defect recovery caused by
infection control re-introduction based on re-establish conﬁdence between patient and dental hygienist.
Case: The dropout patient is 60 years old female, ﬁrst visit date was Feb, 2006, after complete the implant treatment, she
dropout the implant maintenance for 9 years. She referred to the hospital in April, 2018 caused by pain and smell around
implant. The diagnosis in oral condition was peri-implantitis, peri-implant mucositis, and chronic periodontitis as Stage 4,
Grade A.
Clinical procedures and outcomes: We discussed the cause of dropout was lack of understanding for implant maintenance,
she understood the peri-implantitis can be treated by infection control based on daily plaque control. And sulcus bleeding
index was evaluated by the patient such as no attached bleeding on tooth brush and inter dental brush. After 2 weeks, supra
mucosal plaque control was achieved as no sulcus bleeding index. Subsequently, submucosal plaque control was performed
by non-surgical therapy using carbon curette. Otherwise, no reacted site by non-surgical implant sites as 15,17,24,25 was
performed by open ﬂap debridement. After 1 year, all peri-implant sites demonstrated SBI（-）, BOP（-）, pocket reduction,and bone ﬁll at bone defect area. And all periodontitis sites showed pocket reduction.
Conclusion: Infection control is very important to treat peri-implantitis and also periodontitis, and regular interval recall isnecessary. For the sake of successful implant maintenance, it is most important for dental hygienist to establish goodcommunication and re-establish conﬁdence between patients.

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

222022/10/07

14:12:03

23

The 108th Annual Meeting of the American Academy of Periodontology in collaboration
with the Japanese Academy of Clinical Periodontology,
and the Japanese Society of Periodontology.
Abstracts of JACP/JSP Poster Session
Copyright ©2022 The Japanese Academy of Clinical Periodontology, The Japanese Society of Periodontology.
All rights reserved.
Secretariat：c/o Atalacia.Ltd.
1-775, Funahashi-honmachi, Hirakata, Osaka573-1116, Japan
TEL ＋81- 72-807-3890

JACP/JSP Poster Session AAP2022

book JSPJACPポスターセッション2022.indb

232022/10/07

14:12:03