EMA Antibody / MUC1 / Mucin-1 [clone MUC1/845] (V2371)
Catalog No.

Formulation

Size

V2371-100UG

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

100 ug

V2371-20UG

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

20 ug

V2371SAF-100UG

1 mg/ml in 1X PBS; BSA free, sodium azide free

100 ug

V2371IHC-7ML

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC
use only*

7 ml

Bulk quote request
Species Reactivity HumanFormat

Purified

Clonality

Monoclonal (mouse origin)

Isotype

Mouse IgG1, kappa

Clone Name

MUC1/845

PurityProtein G affinity chromatography

Buffer

1X PBS, pH 7.4

Gene ID

4582

Localization

Cytoplasmic and cell surface

Applications

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Limitations

This EMA antibody is available for research use only.

Formalin-fixed, paraffin-embedded human ovariancarcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human colon
carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human endometrial
carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human breast
carcinoma stained with MUC1 / EMA antibody(MUC1/845).

Formalin-fixed, paraffin-embedded human breast
carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human breast
carcinoma stained with EMA antibody (MUC1/845).

SDS-PAGE Analysis of Purified, BSA-Free EMA Antibody(clone MUC1/845). Confirmation of Integrity and Purityof the Antibody.

Description
This antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1 (Mucin-1) orEMA (epithelial membrane antigen). The alpha subunit has cell adhesive properties. It can act both as an adhesion and ananti-adhesion protein. MUC1 / Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme
attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling through phosphorylations andprotein-protein interactions. In immunohistochemical assays, the MUC1 / EMA antibody superbly stains routine
formalin/paraffin carcinoma tissues. MUC1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci ofcarcinoma in bone marrow or liver.

Application Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates
may require the EMA antibody to be titered up or down for optimal performance.
1. HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 10-20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (ifrequired), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen
Human milk fat globule membranes were used as the immunogen for this EMA antibody.

Storage
Store the EMA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

References (1)Ordering: Phone:858.663.9055 | Fax:1.267.821.0800 | Email:info@nsjbio.com

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