University of Tsukuba
Shimoda Marine Research Center

Annual Report
2022

March 2023

Overview of our activities
It has been three years since the emergence of the new coronavirus, and I feel that 2022 has been ayear in which activities to restore normalcy have been promoted, albeit gradually. In relation to ouractivities, we can see from the list of achievements that the weight of activities such as conferences
is shifting from online-based to on-site format, and that for the first time in a long time, there are more
opportunities to meet researchers in person, listen to their presentations, and discuss them in person.
On the other hand, as if aiming for such events, major peaks of infection come several times a year,
and each time they are accompanied by a sense of anxiety.

The military invasion of Ukraine by Russia that began at the end of FY2021 had such a strong impact
on me that words cannot describe it. The situation that I had thought "no way, no way" became a
reality, and I was greatly shaken every time I saw the situation reported in the media day after day. I
have learned about past wars and conflicts from various perspectives and the global framework forcountering them in my educational program, but I was reminded once again that peace is not
permanent and that tireless efforts are needed to maintain it. As of this writing, we are still no closer
to an end. Conflicts are also occurring in other parts of the world. I cannot help but hope that these
problems will be resolved as soon as possible, and that conflict will be eliminated from the world.

Yasunori Sasakura, Director, Shimoda Marine Research Center

1Summary
Overview of the Center
The University of Tsukuba's Shimoda Marine Research Center is a research and education facility formarine biology surrounded by the rich marine environment of the southern Izu Peninsula. The ocean
is the source of life on earth and has given birth to biological diversity. The 21st century is the era oflife science and the environment. The Shimoda Marine Research Center conducts research andeducation in basic and advanced sciences related to marine life in order to deepen our understandingof the basic principles of life and the interactions between organisms.

The center has 10 faculty members who conduct research on marine organisms from various
perspectives ranging from molecular to ecological, including molecular biology, cell biology,
physiology, developmental biology, taxonomy, animal behavior, and ecology. In addition, many
researchers from universities and research institutes in Japan and abroad visit and develop
collaborative research projects. Marine biology is important not only for those who wish to pursue
biology, but also for the development of human resources in a wide range of fields. At the center,
students from the University of Tsukuba's Biology Department and Life and Earth Sciences ResearchGroup, as well as other universities in Japan and abroad, participate in fieldcourses on a variety ofmarine-related topics.

Location and Environmental Conditions
The center is located at the end of Oura Bay, a branch of Shimoda Bay, to the south of Shimoda City
center. The bay is immediately surrounded by the Kuroshio Current, but within the bay there is a small
portion of an inner bay environment. Because of its location at the interface between the temperateand subtropical zones, kelp forests, which represent the temperate zone, coexist with reef-building
corals that inhabit the tropics. In the future, this ecosystem is expected to change drastically due toclimate change and other factors.

Marine organisms used at the center include: the tunicates Ciona intestinalis and Styela sp., the2

solitary ascidian Herdmania momus, the urchins Hemicentrotus pulcherrimus, Temnopleurus reevesii,
Heliocidaris crassispina, Pseudocentrotus depressus, and Diadema setosum, Comatulida crinoids,
nudibranchs, Placazoa, Xenoturbella sp., Acoelomorpha, Brachiopoda, flatworms, the comb jelly
Bolinopsis mikado, corals, the flatfish Pseudopleuronectes yokohamae, sole, and Paralichthys
olivaceus, the wrasse Thalassoma cupido, the spiny lobster Panulirus japonicus, the conch Strombus
luhuanus, the kelp Ecklonia cava, the brown algae Mutimo cylindricus, Sargassum fusiforme, andPetalonia binghamiae.

Facilities and Equipment
The center contains three research and experimental buildings, a fieldcourse building with aquariumand breeding facilities, a workshop, and an accommodation building. The center is equipped with arange of instruments and equipment for diving and sampling for field research and experiments,
various water tanks for conducting indoor experiments, filtered seawater facilities, and equipment for
research in molecular biology, biochemistry, and cell biology. Other facilities include a recombinant
DNA laboratory, a laboratory for breeding laboratory animals (mice), a transgenic animal breedingroom, a seminar room, and a library. Seawater is constantly pumped into tanks from a depth of 3 m
to supply the breeding facilities and laboratories. Vessels are available for biological surveys andcollections using a range of methods, including dredges, trawls, and plankton nets. Shikine-jima
Station, a research facility for the Ocean Acidification Project, is located on Shikine-jima Island (Niijima, Tokyo), about 45 km southeast of the Center, where many researchers from Japan and abroadstay to conduct research and analysis.

Diving Equipment and Facilities
Wetsuits, diving cylinders, masks, snorkels, gloves, boots, and fins

Biological Sampling Equipment
Plankton net, Neuston net, Ekman barge and Smith McIntyre sediment grabs, dredges, sled net, trawl
net, and Niskin bottles.

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Research Equipment
Mass spectrometer TOF-MS, elemental analysis-isotope ratio mass spectrometer, light sheet
microscope, super resolution microscope LatticeSIM, DNA sequencer, PCR equipment (including
quantitative PCR), microplate reader, various optical microscopes, confocal laser microscope,electron microscope (TEM, SEM), soft x-ray imaging systems, calcium imaging systems, high-speed
video cameras, HPLC, CTD sensors, and ultra-low temperature chambers.

Accommodation
For interns, resident graduate students, and visitors, there are two accommodation buildings, the twostory W building and the three-story E building, which can accommodate a total of 85 people. A
cafeteria, bathrooms, and lounge are provided, and on weekdays three meals are served (uponrequest).

4

List of Buildings and Facilities
Shimoda Marine Research Center
Research Building 1

Building (Total 3,931m2)

3-story reinforced concrete building (10 laboratories, 9 experimental rooms, 2
observation and measurement rooms, 2 electron microscope rooms, 1 specimenroom, 1 library, 1 seminar room, 1 low-temperature room, 3 dark rooms, 1 printing
room, and 5 other rooms).

Research Building 2

2-story reinforced concrete building (3 laboratories, 5 experimental rooms, 1
lecture room, 1 archive storage room, 1 analysis room, 1 dark room)

Research Building 3

2-story reinforced concrete building (4 laboratories, 1 experimental room, 1
collaborative analysis room, 1 collaborative research space, 1 conference/seminarroom, 1 measuring equipment room, and 3 other rooms)

Fieldcourse Building

1-story reinforced concrete building (1 large fieldcourse room, 1 indoor mesocosm
facility, and 1 indoor breeding room)

Workshop

1-story reinforced concrete building (1 workshop, 1 storage room, 1 workroom, and1 shower room)

Accommodation

3-story reinforced concrete building (24 Western-style rooms, 3 Japanese-Stylerooms, 1 cafeteria, 2 bathrooms, and 1 lounge)

Vessels

Tsukuba II (19t, 612 HP×2, 40-person capacity)
Karetta (0.5t, 9.9 HP, 6-person capacity)
SMRC (Inflatable boat, 8 HP, 4-person capacity)
Oberia (Rowing boat, 2-person capacity)

Seawater facilities

Fresh seawater is constantly pumped from a depth of 3 m to a 56-ton tank locatedapproximately 13 m above sea level, and is supplied non-stop to the indoor andoutdoor breeding facilities and laboratories in each research building.

Shikine-jima Station

Building (total 149.6m2)

Experimental facilities

1-story building of wood and stone, with a galvanized steel sheet roof. Building The
facilities are equipped with a fridge, freezer, drying oven, a microscope, aworkbench, a sink, and various other laboratory equipment.

Accommodation

Wooden one-story building with galvanized steel plate roof (2 Western-style rooms,
2 Japanese-style rooms, 1 dining room, and 1 bathroom).

Vessels

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Akane (0.5t, 20hp, 7-person capacity).

Staff Members in the Center
Faculty

Director, Professor
Professor
Professor
Associate Professor
Associate Professor
Assistant Professor
Assistant Professor

Technician

笹倉 靖徳
Yasunori SASAKURA
稲葉 ⼀男
Kazuo INABA
Jason HALL-SPENCER
⾕⼝ 俊介
Shunsuke YAGUCHI
中野 裕昭
Hiroaki NAKANO
柴 ⼩菊
Kogiku SHIBA
和⽥ 茂樹
Shigeki WADA

Environmental Ecology
Developmental Biology
Evolutionary embryology
Cell Biology
Marine Ecology

Sylvain AGOSTINI

Marine Biology

Assistant Professor

Ben HARVEY

Environmental Ecology

Assistant Professor

Lucia PORZIO

Environmental Ecology

柴⽥ ⼤輔

Manabu OOUE
⼩髙 友実
Tomomi KODAKA髙野 治朗
Jiro TAKANO
中村 千華
Chika NAKAMURA⾕⼝ 順⼦
Junko YAGUCHI
北之坊 誠也
Seiya KITANOBODavide SPATAFORA

6

Cell Biology

Assistant Professor

Daisuke SHIBATA
⼤植 学

Researcher

Developmental Genetics

柴⽥ あいかPart-time

Researcher

Aika SHIBATA
笹倉 暁⼦
鈴⽊ 智佳

Administration

Head

⽻⼦⽥ 誠

Administration

Administrative assistant

⼟屋 理恵
⼟屋 富⼠⼦Part-time

Administrative assistantGeorge NORTHEN

Part-time

Cleaning Staff

⼭⽥ 順⼦
⽥中 ⽂⼦
秋⼭ 佳⼦
渡邉 恵

Part-time

Research Technician

佐藤 壽彦
⼤畑 雅江
⽥⼦内 加代⽥中 佐貴⼦
⾦守 美⾥
浅野 美世
中尾 菜穂
中野 亜⼦
森下 秀⼦
加納 穂澄
北之坊 仁美⽊村 智美
古屋 こさと
武富 晋⼀郎
⼟屋 絵⾥

JSPS

Postdoctoral Fellow

寺内 菜々
⾹川 理
Enrolled between 2022.4.1-2023.3.31

7

Research Activities
1) Research Introduction
Marine Molecular Biology Division
Genetics (Sasakura)
Ascidians are closest living relatives of vertebrates. Ciona intestinalis is the model ascidian becauseof its well-annotated genome information and established methodologies for manipulating genes.
Using Ciona, our group studies genetic mechanisms underlying development of ascidians. Particularly,
we are interested in the mechanisms of metamorphosis, in which Ciona dramatically converts its bodystructure from swimming tadpole larva into sessile, vase-like adult. We developed the methods tomodify genomes of Ciona by means of transgenic and genome editing technologies. The transgenic
and mutant lines established by the methods are useful tools to observe cellular and molecular
phenomena during development. We are engaged in the National BioResource Project of Ciona,which is purposed to collect, store and provide these useful lines to researchers all over the world. A
constructs for providing them to researchers upon request.
Research Themes:
・ Developmental genetics of ascidians・ Neurodevelopmental and physiological studies on ascidian metamorphosis mechanisms

Cell Biology (Inaba・Shiba)
Cilia and flagella are important cellular organelles for locomotion and fluid flow in body. The structureof cilia and flagella is well conserved in the process of evolution. We study the structure, function andevolution of cilia and flagella by using marine organisms such as ascidian, sea urchin, fish, and comb
jelly. Our research topics cover a wide range of subjects, including fertilization, morphogenesis,
locomotion, evolution and ecology of marine unicellular and multicellular organisms.
Research Themes:
・ Studies on the structure, function and evolution of eukaryotic flagella and cilia
・ Research on sperm motility regulation and adaptation to the fertilization environment
・ Genomics and proteomics in marine invertebrates

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Developmental Biology (Yaguchi)
The main research goal of our laboratory is understanding the molecular mechanisms of body axis
formation and neurogenesis in sea urchin embryos/larvae. We also focus on the function of nervous
system in sea urchin larvae.
Research Themes:
・ Analysis of the mechanisms of body-axis formation and neurogenesis in early embryos
・ Analysis of neural function during early development
・ Analysis of the effects of external environmental stimuli such as light and temperature on
development

Evolutionary Embryology (Nakano)
There are many extant animals, such as xenacoelomophs and placozoans, that are evolutionarily
important but have not been extensively studied. In our group, we perform morphological, ecological,
and developmental research on these ‘non-model organisms’ with the aim of gaining new insights on
the origins, evolution, and diversity of bilaterians and metazoans.
Research Themes:
・ Studies on evolution and diversity of metazoans using flatworms, xenacoelomophs andplacozoans
・ Zoological and natural history research on marine invertebrates
・ Evolutionary and phylogenetic studies of body color diversity in sea slugs
・ Evolutionary developmental studies on brachiopods, including analysis of their shell proteins9Marine Ecology Division
Environmental Ecology (Hall-Spencer and Porzio)
As well as warming the oceans, CO2 released due to human activities is also causing ocean
acidification, which alters the fundamental chemical balance of surface waters (e.g. reducing theamount of carbonate). Our laboratory uses natural analogues of ocean acidification (CO2 seep) inJapan and Europe alongside manipulative experiments to understand the eco-physiologicalprocesses involved in the response of winner and loser species to ocean acidification and warmingin Japan and on coastal ecosystems worldwide. We mostly focus on marine macrophytes as they are
key organisms for the structure and functioning of coastal ecosystems. The aim will be to improve theknowledge on the impacts of combined ocean acidification and global warming on the fate of theirdistribution and the associated ecosystem services.
Research Themes:
・Diversity of macrophytes in coastal systems
・Community structure and functioning under ocean acidification
・Eco-physiological and biological response of macroalgae to global warming and acidification

Environmental Ecology (Harvey)
We seek to understand how changes in environmental conditions (focussing on ocean acidification,
ocean warming, and marine heatwaves) will change our oceans. Our research is multidisciplinary,combining field-based (subtidal and intertidal surveys and experiments), aquarium-based
manipulative experiments, and desk-based (environmental modelling, statistical modelling, metaanalyses) approaches. Our research covers a wide range of subjects within the context of climatechange, including species ecophysiology, biomineralisation, population genetics, biodiversity andcommunity meta-barcoding, community-level structuring processes and interactions, regime shifts
and stability, and ecosystem functioning, goods and services. Taken together, this will allow us tobetter understand the impacts of global climate change on coastal ecosystems worldwide.
Research Themes:・Role of ocean acidification and warming on biodiversity, community structuring, and stability
・Impacts of ocean acidification and warming on calcification, physiology, and functioning
・Impacts of marine heatwaves on biogeographic distribution, range shifts, and aquaculture10Material Cycling (Wada)
Marine organisms and their ambient environments have various interactions. Analysis of theinteractions will allow us to understand the principle of mechanisms of marine ecosystems. In addition,
we can contribute to predict the progress of global climate change such as ocean acidification.
Research Themes:・Assessment of Blue Carbon in Seagrass beds
・Impacts of ocean acidification on coastal ecosystems
・Analysis of marine snow dynamics

Marine Complex Biology Division
Environmental Ecophysiology (Agostini)
We study the ecophysiology of marine organisms with a special focus on corals and the impact ofanthropogenic stressors on their community. Ocean warming is driving the degradation of coral reefs
in the tropic and could allow the increase of coral abundance in warm-temperate areas. However,ocean warming comes in combination with ocean acidification which is limiting the growth of corals
on a global scale. There are many dangers beyond climate change and ocean acidification. Due tothe lack of data on microplastic pollution, which has been attracting attention in recent years, it is not
yet possible to assess what kind of impact it has on marine ecosystems and corals. We also study
the impact of other anthropogenic stressors on marine ecosystems in general and thrive to understandthe ecology and future trajectories of these ecosystems through the study of the physiology andecology combining field and laboratory studies. To increase awareness of the different environmental
problems that threatens marine ecosystems we also conduct various social outreach activities.
Research Themes:
・The effects of anthropogenic stressors on marine ecosystems
・Eco-physiology of scleractinian corals and marine organisms
・Tropicalization of marine ecosystems under ocean acidification and warming
・Evaluation of the impact of microplastic pollution

112) Research Supervision of Students at the Center
University of Tsukuba, Life and Environmental Sciences, Biology
Undergraduate, 4th Year

マガキガイ体内受精における異型精⼦の役割に関する研究

Undergraduate, 4th Year

海洋酸性化と異なる光強度がサンゴ暖温帯サンゴのエンタクミドリ
イシに与える影響
Effects of ocean acidification under different light availability on thewarm temperate coral Acropora solitaryensis

University of Tsukuba, Faculty of Science and Technology, School of Science and Technology,
Information and Life Sciences, Life and Earth Sciences, Biology Degree Program
Research Student

ホヤの変態制御機構に関する研究

Research Student

ウニ幼⽣における神経形成の分⼦メカニズム解析

Masters, 1st Year

カブトクラゲ櫛板の隔⼩板関連タンパク質の探索

Masters, 1st Year

ホヤの変態制御機構に関する研究

Masters, 1st Year

海藻藻場の溶存態有機物の⽣産

Masters, 1st Year

⽇本の沿岸におけるマイクロプラスチックの分布およびフラックス
Distribution and fluxes of microplastics in Japanese coastal areas

Masters, 2nd Year

ウニ胚原腸形成を司る新規メカニズムの解析

Masters, 2nd Year

海藻由来有機物の微⽣物分解の指標探索

Masters, 2nd Year

熱ストレス下における造礁サンゴの⽇周的な光合成活性と防御機
能 と し て の 光 阻 害 Diurnal photosynthetic activities and theprotective role of photoinhibition in hermatypic corals under heatstressMasters, 2nd Year

潮間帯に⽣息するクモハゼは海洋酸性化に対して耐性を⽰すが、間接効果による負の影響を受ける The intertidal goby Bathygobius
fuscus show some tolerance to ocean acidification but is still affected
through indirect effects

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Masters, 2nd Year群体の形態に基づくハマサンゴ属、ハナヤサイサンゴ属およびアナサンゴモドキ属における遺伝的系統の推定
Predicting genetic lineages of the reef-building corals Porites spp.,
Pocillopora spp. and Millepora spp. based on colony morphology

PhD, 2nd Year

マリンスノーの強度と⽣物ポンプの解析

PhD, 2nd Year

ウミウシの体⾊多様性進化と系統学的研究

PhD, 2nd Year

海洋温暖化・酸性化がサンゴ・藻類の相互作⽤に及ぼす影響
Coral-algae interactions under ocean acidification and warming

133) Publications
Marine Molecular Biology Division
Genetics
Khalturin K, Shunatova N, Shchenkov S, Sasakura Y, Kawamitsu M, Satoh N. Polyzoa is back: The
effect of complete gene sets on the placement of Ectoprocta and Entoprocta. Science Advances,8(26):eabo4400. July 2022.
Sakamoto A, Hozumi A, Shiraishi A, Satake H, Horie T, Sasakura Y. The TRP channel PKD2 isinvolved in sensing the mechanical stimulus of adhesion for initiating metamorphosis in thechordate Ciona. Development, Growth & Differentiation. 64(7):395–408. September 2022.
Sasakura Y, Horie T. Improved Genome Editing in the Ascidian Ciona with CRISPR/Cas9 and TALEN.
In: Hatada I, editor. Genome Editing in Animals. New York, NY: Springer US. p. 375–88. (Methods
in Molecular Biology; vol. 2637). February 2023.
Yamagishi M, Huang T, Hozumi A, Onuma TA, Sasakura Y, Ogasawara M. Differentiation of endostylecells by Nkx2-1 and FoxE in the ascidian Ciona intestinalis type A: insights into shared gene
regulation in glandular- and thyroid-equivalent elements of the chordate endostyle. Cell and Tissue
Research 390:189–205. September 2022.
Yamakawa S, Hayashi Y, Kako K, Sasakura Y, Morino Y, Wada H. Mechanism underlying retinoic
acid-dependent metamorphosis in the starfish. Developmental Biology. 492:119–25. December
2022.

Cell Biology
Hayakawa E, Guzman C, Horiguchi O, Kawano C, Shiraishi A, Mohri K, et al. Mass spectrometry ofshort peptides reveals common features of metazoan peptidergic neurons. Nature Ecology &Evolution. 6(10):1438–48. August 2022.
Ito T, Morita M, Okuno S, Inaba K, Shiba K, Munehara H, et al. Fertilization modes and the evolutionof sperm characteristics in marine fishes: Paired comparisons of externally and internally fertilizing
species. Ecology and Evolution. 12(12): e9562. December 2022.
Jokura K, Sato Y, Shiba K, Inaba K. Two distinct compartments of a ctenophore comb plate provide
structural and functional integrity for the motility of giant multicilia. Current Biology. 32(23): 51445152. October 2022.
Sensui N, Itoh Y, Okura N, Shiba K, Baba SA, Inaba K, et al. Spawning-induced pH increase activates
sperm attraction and fertilization abilities in eggs of the Ascidian, Phallusia philippinensis and Ciona
14intestinalis. International Journal of Molecular Sciences. 24(3):2666. January 2023.
Shiba K, Baba SA, Fujiwara E, Inaba K. Calaxin is required for asymmetric bend initiation andpropagation in sperm flagella. Frontiers in Cell and Developmental Biology. 11:1136404. March2023.
Shibata D, Morita M, Sato Y, Shiba K, Kitanobo S, Yokoya R, et al. Axonemal growth and alignment
during paraspermatogenesis in the marine gastropod Strombus luhuanus. Frontiers in Cell andDevelopmental Biology. 10:905748. June 2022.
Sugiura K, Shiba K, Inaba K, Matsumoto M. Morphological differences in tardigrade spermatozoa
induce variation in gamete motility. BMC Zoology. 7(1):8. December 2022.
Hazraty-Kari S, Tavakoli-Kolour P, Kitanobo S, Nakamura T, Morita M. Adaptations by the coral
Acropora tenuis confer resilience to future thermal stress. Communications Biology 14, 5(1):1371.
December 2022.
Ito S, Yamazaki D, Kameda Y, Kagawa O, Ye B, Saito T, Kimura K, Chiba S, Hirano T. Taxonomic
insights and evolutionary history in East Asian terrestrial slugs of the genus Meghimatium.
Molecular Phylogenetics and Evolution. 2023 May 1, 182:107730.
Chiba M, Hirano T, Yamazaki D, Ye B, Ito S, Kagawa O, Endo K, Nishida S, Hara S, Aratake K, Chiba
S. The mutual history of Schlegel’s Japanese gecko (Reptilia: Squamata: Gekkonidae) and humans
inscribed in genes and ancient literature. PNAS Nexus. 2022 Nov, 1, (5):pgac245.
Kudo K, Kagawa O, Ito S, Wada S, Nishi H, Shariar SM, Yamazaki D, Hirano T, Chiba S. Species
identification and invasion pathways of an introduced snail Macrochlamys sp. Japan. BioInvasions
Records. 2022 Sep (4): 839–854

Developmental Biology
Kinjo S, Kiyomoto M, Suzuki H, Yamamoto T, Ikeo K & Yaguchi S. TrBase: A genome andtranscriptome

databaseof Temnopleurus

reevesii. Development,

Growth&Differentiation.64(4):210-218. May 2022.

Evolutionary Embryology
Kushida Y, Imahara Y, Wee HB, Fernandez-Silva I, Fromont J, Gomez O, et al. Exploring the trendsof adaptation and evolution of sclerites with regards to habitat depth in sea pens. PeerJ. 10:e13929.
September 2022.
Nakano H, Nakano A, Maeno A, Thorndyke MC. Induced spawning with gamete release from bodyruptures during reproduction of Xenoturbella bocki. Communications Biology. 6(1):172. February
2023.

15

Nakano H. Dawn of Placozoan Phylogenetic Taxonomy. In: 100th Anniversary Publication Committee,
Seto Marine Biological Laboratory, Kyoto University, editors. Marine Invertebrate Biodiversity.
Kyoto: Kyoto University Academic Press; 2022. p. 28–38.

16

Marine Ecology Division
Environmental Ecology
Heitzman JM, Caputo N, Yang SY, Harvey BP, Agostini S. Recurrent disease outbreak in a warm
temperate marginal coral community. Marine Pollution Bulletin. 182:113954. September 2022.
Hudson CJ, Agostini S, Wada S, Hall-Spencer JM, Connell SD, Harvey BP. Ocean acidification
increases the impact of typhoons on algal communities. Science of The Total Environment.
865:161269. March 2023.
Kerfahi D, Harvey BP, Kim H, Yang Y, Adams JM, Hall-Spencer JM. Whole community and functional
gene changes of biofilms on marine plastic debris in response to ocean acidification. Microbial
Ecology. 85(4):1202–14. April 2022.
Seto M, Harvey BP, Wada S, Agostini S. Potential ecosystem regime shift resulting from elevated CO2
and inhibition of macroalgal recruitment by turf algae. Theoretical Ecology. 16:1–12. January 2023.

Materials Cycling
Hudson CJ, Agostini S, Wada S, Hall-Spencer JM, Connell SD, Harvey BP. Ocean acidification
increases the impact of typhoons on algal communities. Science of The Total Environment.
865:161269. March 2023.
Seto M, Harvey BP, Wada S, Agostini S. Potential ecosystem regime shift resulting from elevated CO2
and inhibition of macroalgal recruitment by turf algae. Theoretical Ecology. 16:1–12. January 2023.
Wada S, Satoh Y, Hama T. Massive loss and microbial decomposition in reproductive biomass of
Zostera marina. Estuarine, Coastal and Shelf Science. 275:107986. September 2022.

Marine Complex Biology Division
Environmental Ecophysiology
Heitzman JM, Caputo N, Yang SY, Harvey BP, Agostini S. Recurrent disease outbreak in a warm
temperate marginal coral community. Marine Pollution Bulletin. 182:113954. September 2022.
Hudson CJ, Agostini S, Wada S, Hall-Spencer JM, Connell SD, Harvey BP. Ocean acidification
increases the impact of typhoons on algal communities. Science of The Total Environment.
865:161269. March 2023.
Seto M, Harvey BP, Wada S, Agostini S. Potential ecosystem regime shift resulting from elevated CO2
and inhibition of macroalgal recruitment by turf algae. Theoretical Ecology. 16:1–12. January 2023.

17

Technical Staff
髙野治朗, Agostini S, Ramtahal J, 濱野章⼀, 桂川英徳, 鷲尾正彦, 阿部広和, ⼩⽊曽正造, 渡部雪菜
下⾕豊和, パトゥイエ 由美⼦, Tara-JAMBIO マイクロプラスチック共同調査(東⽇本・北陸編), 臨

海・臨湖, 39: 1-6. November 2022.
Shibata D, Morita M, Sato Y, Shiba K, Kitanobo S, Yokoya R, et al. Axonemal growth and alignment
during paraspermatogenesis in the marine gastropod Strombus luhuanus. Frontiers in Cell andDevelopmental Biology. 10:905748. June 2022.

18

4) Conference Presentations and Invited Lectures
Marine Molecular Biology Division
Genetics【International Conference】〇Yasunori Sasakura, Akiko Hozumi, Arata Onodera, Nozomu M.
Totsuka, Honoo Satake, Kohji Hotta, Takeo Horie, G-protein signaling relay promotes Ciona
metamorphosis. 11th Tunicate Meeting, Konan University, Kobe, Hyogo, 2022.07.13【International Conference】〇Gabriel Krasovec, Akiko Hozumi, Tomoyuki Yoshida, Takayuki Obita,
Mayuko Hamada, Akira Shiraishi, Honoo Satake, Takeo Horie, Hisashi Mori, Yasunori
Sasakura, D-serine controls epidermal vesicle release via NMDA receptor allowing tissue
migration during the metamorphosis of Ciona. 11th Tunicate Meeting, Konan University, Kobe,
Hyogo, 2022.07.13【Oral Presentation】⼩野寺新、保住暁⼦、⼾塚望、佐⽵炎、濱⽥⿇友⼦、堀江健⽣、堀⽥耕司、〇笹倉靖徳, ホヤの固着依存的な変態開始を司る G タンパク質シグナル経路, 第 45 回⽇本分⼦⽣物学会, 幕張メッセ，千葉県千葉市, 2022.12.1【Poster Presentation】〇⼩野寺新、保住暁⼦、⼾塚望、佐⽵炎、濱⽥⿇友⼦、堀江健⽣、堀⽥耕司、笹倉靖徳, ホヤの固着依存的な変態開始を司る G タンパク質シグナル経路, 第 45 回⽇本
分⼦⽣物学会, 幕張メッセ，千葉県千葉市, 2022.12.1【Poster Presentation】〇笹倉靖徳、佐藤ゆたか、吉⽥学, NBRP カタユウレイボヤ, 第 45 回⽇本
分⼦⽣物学会, 幕張メッセ，千葉県千葉市, 2022.11.30-12.2

Cell Biology【Invited Lecture】柴⼩菊, 精⼦⾛化性における遊泳⽅向制御機構の解析, ⽇本顕微鏡学会第78回学術講演会シンポジウム「受精・⽣殖研究の最前線」,

ビッグパレットふくしま, 福島県郡⼭市, 2022.5.13【Oral Presentation】〇柴⼩菊，稲葉⼀男, 3D 遊泳トラッキングシステムによるホヤ精⼦⾛化性の解析, ⽇本動物学会第93回早稲⽥⼤会2022, 早稲⽥⼤学, 東京都早稲⽥, 2022. 9.8-10【Oral Presentation】柴⽥ ⼤輔，守⽥ 昌哉，佐藤 友，柴 ⼩菊，北之坊 誠也，横屋 稜，〇稲葉

19

⼀男, マガキガイ異型精⼦形成における軸⽷の形成と配向, ⽇本動物学会第93回早稲⽥⼤会
2022, 早稲⽥⼤学, 東京都早稲⽥, 2022. 9.8-10【Oral Presentation】〇岩本 裕之，城倉 圭，⼤岩 和弘，稲葉 ⼀男, クシクラゲ櫛板の X 線回折像から繊⽑軸⽷の 3 次元構造を復元する, ⽇本動物学会第 93 回早稲⽥⼤会 2022, 早稲⽥⼤学,
東京都早稲⽥, 2022. 9.8-10【Oral Presentation】○北之坊 誠也，古川 真央， ⼤⽊ 駿，Sanaz Hazraty-Kari, Parviz Tavakoli-Kolour,
Tanya Singh，守⽥ 昌哉, 交雑するミドリイシ属サンゴ属の 系統解析と遺伝⼦浸透について,
⽇本動物学会第 93 回早稲⽥⼤会 2022, 早稲⽥⼤学, 東京都早稲⽥, 2022. 9.8-10【Poster Presentation】Kogiku Shiba, Kazuo Inaba, ピエゾ駆動対物レンズを⽤いたホヤ精⼦遊泳の
3 次元的解析, 3D analysis of ascidian sperm swimming using a piezoelectric Z-scanner
attached to a microscope objective, 第 60 回⽇本⽣物物理学会年会, 北海道函館市, 2022.9.2830【Poster Presentation】Hiroyuki Iwamoto, Kei Jokura, Kazuhiro Oiwa, Kazuo Inaba, 真核⽣物鞭⽑・繊⽑軸⽷構造の X 線回折トモグラフィー：クシクラゲ櫛板の利⽤, X-ray diffraction-based
computed tomography of axonemal structure of eukaryotic flagella/cilia:Use of Ctenophorecomb plates, 第 60 回⽇本⽣物物理学会年会, 北海道函館市, 2022.9.28-30【Oral Presentation】○柴 ⼩菊，稲葉 ⼀男, ピエゾ駆動対物レンズを⽤いたホヤ精⼦鞭⽑運動の 3次元的解析, 第 12 回繊⽑研究会, 名古屋市⽴⼤学, 愛知県名古屋市, 2022.10.30-11.1【Poster Presentation】〇北之坊誠也, 古川真央, ⼤⽊駿, ⼾篠祥, Sanaz Hazraty-Kari, Parviz
Tavakoli-Kolour, Tanya Singh, 守⽥昌哉, 同所的に⽣息するミドリイシ属サンゴ交雑検出の試
み −Gras-di®解析を⽤いて−, "An attempt to detect sympatric Acropora coral hybridization
using Gras-di analysis, ⽇本サンゴ礁学会第 25 回⽯垣⼤会, ⽯垣市市⺠会館中ホール, 沖縄県
⽯垣市, 2022.11.11-13【Oral Presentation】○稲葉⼀男、城倉圭、佐藤友、⼩坂実央、柴⼩菊, クシクラゲ櫛板内で軸⽷集合体が波打つための分⼦基盤, 2023 年⽣体運動研究合同班会議, 東京⼤学本郷キャンパス⼩柴ホール, 東京都⽂京区, 2023.1.6-8【Oral Presentation】 ○柴⽥あいか、野間泉、松川将之、⾼橋⽂雄、笠原賢洋, 共⽣藻によるミドリ
ゾウリムシ光運動反応制御, 2023 年⽣体運動研究合同班会議, 東京⼤学本郷キャンパス⼩柴
ホール, 東京都⽂京区, 2023.1.6-8
20【Oral Presentation】 ○岩本裕之、稲葉⼀男、⼤岩和弘, クシクラゲ櫛板の繊⽑打に伴う軸⽷構造変化を⾼速 X 線回折測定で⾒る, 2023 年⽣体運動研究合同班会議, 東京⼤学本郷キャンパス⼩柴ホール, 東京都⽂京区, 2023.1.6-8【International Conference/Oral Presentation】Kazuo Inaba, Osamu Kutomi, Sayaka Yamaguchi,
Seiya Kitanobo, Kogiku Shiba, Katsutoshi Mizuno, Calaxin-mediated regulation of ciliary motility,
Cold Spring Harbor Asia Conference on Cilia & Centrosomes, Awaji Yumebutai ConferenceCenter, Awaji, Hyogo, Japan, Feb 28 - Mar 03, 2023
【Invited Lecture】 柴⼩菊, 海産⽣物を⽤いた鞭⽑繊⽑運動マシナリーの解明, 第⼀回ジオラマ⾏
動⼒学・散乱透視学合同シンポジウム「ジオラマ透視学」, 基礎⽣物学研究所地下1階バイト
レミーティングルーム, 愛知県岡崎市, 2023.3.22-23

Developmental Biology【Invited Lecture】Shunsuke Yaguchi. Planktonic sea urchin larvae change their swimming direction
in response to strong photoirradiation. Developmental Biology of the Sea Urchin XXVI. オンラ
イン 2022.4.5-9【Invited Lecture】⾕⼝俊介. WestPac-SUGDB: A genome and transcriptome database of Western
Pacific sea urchins. シンポジウム「海産無脊椎動物」⽇本動物学会⼤９３回⼤会、早稲⽥⼤学、
東京

2022. 9.8-10【Invited Lecture】Shunsuke Yaguchi. Light regulates larval behaviors of sea urchins. LBDV Special
seminar. LBDV, Villefranche-sur-mer, France. 2022.11.10【Invited Lecture】Shunsuke Yaguchi. Light regulates larval behaviors of sea urchins. Special seminar.
University of Nice, Nice, France. 2022.11.9

Evolutionary Embryology【Poster Presentation】〇林牧⼦、前野哲輝、中野裕昭, イロウミウシ科ウミウシから得られた寄⽣性扁形動物の⼀種の分類学的検討, 第 91 回⽇本寄⽣⾍学会⼤会, とかちプラザ, 北海道帯広市, 2022.5.28-29【Oral Presentation】浅井仁、宮澤秀幸、梁瀬隆⼆、稲葉⼀男、○中野裕昭, 背⾯突起を有する無腸動物の⼀種の分類学的検討, ⽇本動物学会第 93 回早稲⽥⼤会 2022, 早稲⽥⼤学, 東京都早稲21

⽥, 2022. 9.8-10【Invited Lecture】 中野裕昭, 知られずに⽣きている海の動物たちの研究, ⽇本動物学会第 93 回早稲⽥⼤会 2022, 早稲⽥⼤学, 東京都早稲⽥, 2022. 9.8-10【Invited Lecture】 ○塙宗継、中野裕昭, 平板動物のキメラ形成を介した性成熟と多様性の獲得の可能性, ⽇本動物学会第 93 回早稲⽥⼤会 2022, 早稲⽥⼤学, 東京都早稲⽥, 2022. 9.8-10

Marine Ecology Division
Environmental Ecology【Invited Lecture】Ben P. Harvey. “Rise of the turfs: the simplification of marine ecosystems under
ocean acidification”. Newcastle University, UK, 2022.4.26.
【International Conference】〇Ben P. Harvey, Ro Allen, Sylvain Agostini, Linn J. Hoffmann, Koetsu
Kon, Tina C. Summerfield, Shigeki Wada & Jason M. Hall-Spencer ““Feedback mechanisms
stabilize degraded turf algal systems at a CO2 seep site” Oral presentation at the 5thInternational Symposium on The Ocean In A High CO2 World, Lima, Peru, 2022.9.15.
【International Conference】〇Ben Harvey, Sean Connell, Sylvain Agostini, Fabio Badalamenti,
Marco Milazzo, Jason Hall-Spencer, Ivan Nagelkerken, Shigeki Wada, Bayden Russell“Functional decline of sea urchins due to ocean acidification can mediate changes in biogenic
habitat” Oral presentation at the 23rd International Temperate Reef Symposium, Hobart,
Tasmania, Australia, 2023.1.9.
【International Conference】〇Callum Hudson, Sylvain Agostini, Shigeki Wada, Jason Hall-Spencer,
Sean Connell, Ben P. Harvey “Ocean acidification increases the impact of typhoons on algal
communities” Oral presentation at the 23rd International Temperate Reef Symposium, Hobart,
Tasmania, Australia, 2023.1.10.

Materials Cycling【Invited Lecture】和⽥茂樹, ⼆酸化炭素の増えた未来の海で⽣態系の変化を探る, 第7回海中海底
⼯学フォーラム・ZERO, 東京⼤学⽣産技術研究所, 東京都⽬⿊区, 2022.4.22【Invited Lecture】和⽥茂樹, ⾃然の⾼CO2海域を利⽤した付着⽣物群集・⽣態系の将来予測, ⽇本
付着⽣物学会50周年シンポジウム, 東京⼤学⽣産技術研究所⼤気海洋研究所講堂, 千葉県柏22

市, 2022.10.6-7【International Conference】Shigeki Wada, Yuhi Sato, Selective aggregation with bubbles on sea
surface, ISBEC 2023, University of Tsukuba, Tsukuba, Ibaraki, 2023.3.8-9【Invited Lecture】和⽥茂樹, 未来の海を使って⽣態系の将来を探る, 海洋⽣物シンポジウム2023,
東京海洋⼤学⽩鷹館, 東京都⽬⿊区, 2022.4.22

Marine Complex Biology Division
Environmental Ecophysiology【Oral Presentation】〇Guinther Mitusashi, Yuko F. Kitano, Hume Benjamin C. C, Armstrong, Eric,
Barbara Porro, Emilie Boissin, Julie Poulain, et al. “Prediction of Genetic Lineages Using Coral
Colony Morphology.” Oral presented at the ⽇本地球惑星科学連合 2022 年⼤会, online, May
27, 2022.
【International Conference】〇Guinther Mitusashi, Yuko F. Kitano, Hume Benjamin C. C, Armstrong,
Eric, Barbara Porro, Emilie Boissin, Julie Poulain, et al.. “Skeleton Morphometric Analysis ofReef-Building Corals for Taxonomic Identification and Genotype Prediction.” Oral presented at
the 15th International Coral Reef Symposium, online, July 3, 2022.
【Oral Presentation】〇Ramtahal, Jonathan, Kugako Sugimoto, Tara Jambio Consortium, Yumiko
Patouillet, and Sylvain Agostini. “TARA-JAMBIO Joint Microplastic Survey: Distribution, Fluxand Plastisphere of Microplastics in Japanese Coastal Areas.” Oral presented at the Japan
Geoscience Union Meeting 2022, Makuhari Messe, Chiba, Japan, May 22, 2022.
https://confit.atlas.jp/guide/event/jpgu2022/subject/MIS19-05/advanced.
【 Invited Lecture 】 〇 Riccardo Rodolfo-Metalpa, Verena Schoepf, Sylvain Agostini, Jean-Pierre
Gattuso, Raquel Peixoto, and David Obura. “Quels moyens avons-nous pour ameliorerl’adaptation rapide et la resilience des recifs coraliens?” Oral presented at the COP27, SharmEl Sheik, Egypt, November 11, 2022.
【 Invited Lecture 】 〇 Riccardo Rodolfo-Metalpa, Verena Schoepf, Sylvain Agostini, Jean-Pierre
Gattuso, Raquel Peixoto, and David Obura.

“What Tools Do We Have to Improve Rapid

Adaptation and Resilience of Corals? A Global Approach with Specific Perspectives for the23

Pacific Region.” Oral presented at the COP27, Sharm El Sheik, Egypt, November 10, 2022.
【Invited Lecture】Sylvain Agostini. “Mission Tara Jambio

マイクロプラスチック共同調査.” Oralpresented at the 令和 3 年度海洋プラスチックごみ学術シンポジウム, online, March 11, 2022.
http://www.env.go.jp/water/post_125.html.
【 Invited Lecture 】 Sylvain Agostini. “Tara Jambio Microplastic Joint Survey: Science, Art and
Education.” Oral presented at the GEA International Conference 2022, Tokyo, Japan, October27, 2022.
https://www.gea.or.jp/en/2022/gea22.html.
【International Conference】〇Sylvain Agostini, Ben P. Harvey, Shigeki Wada, Koetsu Kon, Joshua
M. Heitzman, Nicolas Floc’h, Marco Milazzo, et al. “What We Learn from the Shikine CO2 Seepon the Effects of High CO2 on Hermatypic Corals.” Oral presented at the 5th International
Symposium on The Ocean in A High CO2 World, Lima, Peru, September 15, 2022.
【Oral Presentation】〇Joshua M. Heitzman, Hirata, A., Mitushasi, Guinther, and Agostini, Sylvain.
“Coral-Algae Interactions under Ocean Acidification.” Oral presented at the 25th JapaneseCoral Reef Symposium, Ishigaki, Japan, November 12, 2022.
【Oral Presentation】〇Joshua M. Heitzman, Hirata, A., Mitushasi, Guinther, and Agostini, Sylvain.
“Coral-Algae Interactions under Ocean Acidification.” Oral presented at the 5th International
Symposium on The Ocean in A High CO2 World, Lima, Peru, September 15, 2022.

Technical Staff【Oral Presentation】○柴⽥ ⼤輔，⼤畑 雅江，⼩髙 友実，中村 千華，加納 穂澄，北之坊 仁美，⼩坂 実央，柴 ⼩菊，稲葉 ⼀男, カブトクラゲの実験動物化に向けた継代飼育系の確⽴,
⽇本動物学会第93回早稲⽥⼤会2022, 早稲⽥⼤学, 東京都早稲⽥, 2022. 9.8-10【Oral Presentation】〇髙野治朗, Tara-JAMBIO マイクロプラスチック共同調査, 臨海・臨湖実験所・センター技術職員研修会議, 東京⼤学⼤学院理学系研究科付属臨海実験所, 神奈川県三崎,2022.11.9-11【Oral Presentation】〇髙野治朗, 特殊任務への挑戦④〜マイクロプラスチック調査編〜, 第 5 回筑波⼤学技術職員交流会, 筑波⼤学(ハイブリッド), 茨城県つくば市, 2022.3.8

245) Press Releases
https://www.tsukuba.ac.jp/journal/biology-environment/20220524140000.html (Yaguchi)
https://www.atpress.ne.jp/news/349679 TARA JAMBIO ART PROJECT 展(Agostini）

https://www.atpress.ne.jp/news/329344 アーティスト⽇⽐野克彦の作品を中⼼としたTARA展(Agostini）
https://www.atpress.ne.jp/news/307631 科学×アート×教育で海を守る活動に追い⾵を！ アニエスベー
がサポートする タラ オセアン ジャパン 2022年春夏の取り組み(Agostini）

https://www.atpress.ne.jp/news/307101 タラ オセアン Tara JAMBIO Microplastics Project
同調査にかかる資⾦調達プロジェクトを500万円を⽬標に挑戦中 (Agostini）

6) Awards and Prizes
北之坊誠也,⽇本サンゴ礁学会第 25 回⽯垣⼤会, 優秀発表賞, ⽯垣市市⺠会館中ホール, 沖縄県⽯垣市,
2022.11.11-13

7) Newspaper articles and TV coverage
Inaba, 「繊⽑から眺める海の⽣き物」Ebucheb（エブオブ） Vol.86, pp2-5 (2023)
Inaba,

SciTech Dairy, Dec 5, 2022, https://scitechdaily.com/mystery-behind-comb-jellys-

mesmerizing-movement-solved/
Inaba,

EurekAlert,

NEWS

RELEASE

26-OCT-2022,

https://www.eurekalert.org/news-

releases/969941
Inaba, テレビ放映 NHK ヒューマニエンス 「ミクロの⽑” 細胞を指揮する司令塔」2022 年 11 ⽉
1 ⽇放送
Inaba, 筑波⼤学ポッドキャスト No. 021 クラゲだってがんばってます！まっすぐ泳ぐための「クシ」の秘密, 2022 年 11 ⽉ 11 ⽇
Yaguchi, 筑波⼤学ポッドキャスト

No. 003 ⾷べるだけじゃないんです！知られざるウニの世界へようこそ, 2022 年 5 ⽉ 24 ⽇
Nakano，福井新聞『背中に⾓ 無腸類の新種 「オニムチョウウズムシ」』2022.4.19
Nakano, 毎⽇新聞「⽇本沿岸で発⾒、新種⽣物も紹介 ⽩浜・京⼤⽔族館／和歌⼭」 2023.2.1
Nakano, 紀伊⺠報「海洋⽣物を究める ⽩浜京⼤⽔族館で企画展」2023.3.14
Wada、⽇経新聞、海の酸性化、⽣物むしばむ サンゴや⾙に打撃、2022 年 6 ⽉ 18 ⽇
25

Wada、⼭陽新聞、⾥海からの警告 第 2 部 異変 ⑩酸性化の脅威、2023 年 3 ⽉ 18 ⽇
Wada、テレビ放映、NHK、NHK スペシャル、海の異変 しのびよる酸性化の脅威、2022 年 7 ⽉ 17⽇
Wada、テレビ放映、NHK、サイエンスゼロ、⽣き物のからだが溶ける！？”海洋酸性化”の脅威、2022
年 8 ⽉ 14 ⽇
Wada、テレビ放映、NHK、コズミックフロント、海洋酸性化 静かに迫る危機、2023 年 3 ⽉ 2 ⽇
Shibata，テレビ放映 NHK ダーウィンが来た「カラフルでミラクル★海の⼤スター！ヒトデ」，2022
年 8 ⽉ 7 ⽇放送
Agostini、共同通信 特集記事「海中の森が消えた、静岡・伊⾖

海藻⾷べ尽くすブダイやアイゴ」、

2022 年 10 ⽉ 18 ⽇
Agostini、静岡新聞 特集記事「藻場の消失 漁業に打撃」、2022 年 11 ⽉ 1 ⽇

8) International Collaborative Research
USA・University of Connecticut Health, Dr. Stephen M. King,「繊⽑鞭⽑タンパク質の構造、機能に関する研究」 (Inaba)
Czech Republic・University of South Bohemia, Dr. Otomar Linhart,「チョウザメ精⼦のタンパク質の解析」 (Inaba)
Mexico・National Autonomous University of Mexico(UNAM)Dr. ⻄垣卓也,「後⽣動物における精⼦鞭⽑運動制御の共通性と多様性」 (Inaba・Shiba)
USA・Howard Hughes Medical Institute Janelia Research Campus, Dr. Teng-Leong Chew,「ハプト藻運動装置の微細構造に関する研究」 (Inaba)
Switzerland・Paul Scherrer Institute, Dr. Takashi Ishikawa,「軸⽷ダイニンの分⼦構造に関する研究」 (Inaba)
Australia・Deakin University, Dr. Alecia Bellgrove,「褐藻配偶⼦の運動に関する研究」 (Inaba・Shiba)
USA・Arizona State University, Dr. Hu Ke,「サンゴと褐⾍藻の共⽣関係に関する研究」(Inaba)
USA・University of California, Santa Barbara, Dr. William Smith, France・Montpellier Cell Biology
Research Center, Dr. Patrick Lemaire,「ホヤの神経機能に関する研究」(Sasakura)
Ireland・Galway University, Dr. Gabriel Krasovec,

26

「ホヤの変態と D-セリンに関する研究」(Sasakura)
USA・Swarthmore University, Dr. Bradley Davidson, Dr. Hannah Gruner, Dr. CJ Pickett，「ホヤの変態と成体組織構築に関する研究」（Sasakura）
USA・Brown University, Dr. Gary M. Wessel,「棘⽪動物におけるゲノム編集に関する研究」(Yaguchi)
France・Sorbonne University, Dr Jenifer Croce,「ウニ幼⽣の神経形成に関する研究」（Yaguchi）
Sweden・Royal Swedish Academy of Sciences, Dr. Michael C. Thorndyke,「珍渦⾍の発⽣学的研究」(Nakano)
Tara Pacific Consortium, Dr. Serge Planes、 Dr. Denis Allemand、et al.(Agostini)Hong Kong SAR・Hong Kong University, Dr. Bayden Russell,「Transcriptomics of sea urchins in response to warming」(Harvey)
Canada・University of British Columbia, Dr. Chris Harley & Dr. Katie Marshall,「Role of marine heatwaves in determining species biogeographic distribution」(Harvey)
China・Guangdong Ocean University, Dr. 赵⼒强, 「Shell geochemistry of mussels」(Harvey)
Korea・Keimyung University, Dr. Dorsaf Kerfahi,「Role of ocean acidification on biofilm settlement dynamics on plastic substrates」(Harvey・HallSpencer)
New Zealand・Victoria University of Wellington, Dr. Chris Cornwall,「Role of ocean acidification on coralline algae responses」(Harvey・Porzio・Hall-Spencer)

9) Joint Research with Companies
リージョナルフィッシュ株式会社「⽇本市場に受け⼊れられやすいゲノム編集育⼿法の開発」（Yaguchi）

10) Organized Conferences, Symposiums, and Research
Meetings
井尻 貴之, 柴 ⼩菊, 佐藤 裕公, 河野 菜摘⼦, 第 8 回⽣殖若⼿の会, 明治⼤学駿河台キャンパス(ハイブリッド),

27

東京都千代⽥区, 2022.9.10-11

Makoto Iima, Takuji Ishikawa, Kenta Ishimoto, Kenji Kikuchi, Toshiyuki Nakagaki, Yukinori Nishigami,
Kogiku Shiba, One-day Workshop on Cellular-level ethological dynamics towards "proto-intelligence",
Online + Maskawa Hall, Kyoto University , Kyoto, Japan, 29th October, 2022
Takehiro Kusakabe, Takahito Nishikata, Hiroki Nishida, Yasunori Sasakura, 11th International
Tunicate Meeting, Okamoto Campus, Konan University, Okamoto, Kobe, Japan, 11-16th July, 2022

28

Educational Activities
1) Lectures and fieldcourses
University Fieldcourse
PeriodUniversity Department

Subject Area#

2022.6.13-16

⼭梨⼤学

環境⽣物学実習

8

2022.7.11-15

筑波⼤学⽣物学類

動物分類学臨海実習

112022.7.18-22

筑波⼤学⽣物学類

動物分類学臨海実習10

⽣物公開臨海実習（海⼭連携）32022.8.24-26
2022.8.29-9.1

⼭梨⼤学

野外⽣物学実習

11

2022.9.5-9

筑波⼤学⽣物学類

⽔圏⽣物学臨海実習

152022.9.12-16

筑波⼤学⽣物学類

⽣殖⽣物学臨海実習13

筑波⼤学⾃然保護寄附講
座
2022.9.26-30

筑波⼤学理⼯情報⽣命学
術院⽣命地球環境学研究

10
海域フィールド実習
マリン⽣態環境科学群
2022.10.5-7

筑波⼤学⼤学院共通
健康科学⼤学健康科学部

2023.1.23-26

理学療法学科

海洋⽣物の世界と海洋環境講座
海洋⽣物臨海実習

1062023.2.20-23

筑波⼤学

地球環境学野外実験Ⅱ

62023.3.6-10

筑波⼤学⽣物学類

⽔圏⽣物学実習

13

2023.3.20-24

筑波⼤学⽣物学類

植物分類学臨海実習

182023.3.27-29

筑波⼤学⼤学院共通

地球規模課題と国際社会:海洋環境変動と⽣命

629

Lectures and Seminars
PeriodUniversity Department

Subject Area

筑波⼤学理⼯情報⽣命学#2

通年不定期開催

術院⽣命地球環境学研究 マリンバイオロジー特論(オンライン)群
筑波⼤学理⼯情報⽣命学

2022.11.10-11

2

術院⽣命地球環境学研究 マリン分⼦⽣命科学I（オンライン）

群
2022.8.8-10

筑波⼤学⽣物学類

⽣物寺⼦屋2) Demonstrations and workshops
河津町⽴河津中学校 職場体験学習
下⽥市⽴下⽥中学校 職場体験学習
東京国際フランス学園 センター⾒学・仕事体験
Laurus International School of Science

Plankton observation, and research experience3) Academic Activities and Social Contributions
Zoological Science (Associate Editor) （Inaba)
Zoological Letters (Associate Editor)（Inaba）
Invertebrate Reproduction and Development (Editorial Board) （Inaba）
Japanese Association for Marine Biology (JAMBIO, President)（Inaba）
Journal of Experimental Zoology Part A (Editorial Board)（Inaba）
⽇本動物学会理事・会⻑（Inaba）
⽇本動物学会理事（Shiba）
⽇本動物学会男⼥共同参画委員（Shiba）
Development Growth and Differentiation (Editorial Board) (Sasakura)
⽣物科学学会連合地球⽣物プロジェクト委員（Yaguchi）
30

5⽇本動物学会理事（Yaguchi）
⽇本動物学会国際交流委員（Yaguchi）
Scientific Reports (Editorial Board) （Yaguchi）
Development Growth and Differentiation (Guest Editor)（Yaguchi）
幼⿂⽔族館バフンウニ展⽰（Yaguchi・Yaguchi）
⽇本動物学会関東⽀部 ⽀部代表委員（Nakano）
⽇本動物学会関東⽀部 選挙管理委員 委員⻑（Nakano）
⽇本動物学会 ZDW (ZooDiversity Web) 委員（Nakano）
Zoological Science (Guest Associate Editor)（Nakano）
⽩浜⽔族館 企画展（企画運営）（Nakano）
JAMBIO 沿岸⽣物合同調査（担当）（Nakano）
Scientific Reports (Editorial Board) （Nakano）
理科年表 ⽣物部（監修）（Nakano）
Tara Ocean Japan (理事) (Agostini)
⽇本動物学会第 93 回早稲⽥⼤会 2022 動物学ひろば開催 (Shibata・Ooue)

4) Provision of Research Materials
⽥中 克彦 東海⼤学海洋学部海洋⽣物学科, バフンウニ 30 個体塙 宗継

⼭梨⼤学⼤学院総合研究部医学域, アカヒトデ 10 個体、ムラサキウニ 10 個体、
ボウシュウボラ 3 個体、ナマコ 3 個体、ウミシダ 3 個体

5) Public OutreachOpen Lectures
令和 4 年度筑波⼤学公開講座「海洋⽣物学⼊⾨」（⾼校⽣対象）, 2022 年 8 ⽉ 2 ⽇-5 ⽇, 筑波⼤学下⽥臨海実験センター.
Inaba, 「⽇本のマリンステーション連携-JAMBIO の設⽴と活動」, 国際⽣物科学連合（IUBS）第34 回総会公開講演会・第 5 回 JAMBIO 国際シンポジウム, 2023 年３⽉ 11 ⽇, ハイブリッド開催
中央⼤学後楽園キャンパスおよびオンライン配信.
31

Inaba, 「ミクロの⽑のはなし」, 下⽥市⽔産・海洋学講座, 2023 年 3 ⽉ 23 ⽇, 下⽥市⽂化会館.

Events「海洋⽣物を究める！ ―JAMBIO 沿岸⽣物合同調査の紹介―」, JAMBIO 沿岸⽣物合同調査の成果を展⽰する企画展, 2023 年 2 ⽉ 1 ⽇-2023 年 5 ⽉ 14 ⽇, 京都⼤学⽩浜⽔族館.

32

JAMBIO-TARA microplastics survey-related outreach
Date

Event type

Event Description

Number of
Attendees

Tara Jambio Microplastic Exhibition@Gallery Space of Agnes
2022-04-08 Expo

900
b boutique @KyotoBAL,

2022-04-09 Expo

Kyotographie Samuel Bollendorf and other exhibitions

15000

2022-04-10 Event

Kyotographie Talk Session30

2022-04-22 Event

Talk after Screening MPS @uplink kyoto

15

POP UP Exhibition of Tara Ocean Foundation @Kyoto
2022-04-22 Expo

250
Shimpukan
Tara Awashima Club with winner of Tara Poster Concours

2022-04-29 Presentation12
2020-2021
Tara Crowdfunding Reward seminar @agnesb-tara ocean

2022-05-29 Presentation

5
Office2022-06-12 Presentation

Tara Presentation @CCIFJ

40

2022-07-03 Event

Beach + Movie + Discussion @ sado

21

2022-08-20 Expo

SDGs Expo Himeji2022-08-20 Event

Panel discussion @ Himeji

802022-08-26 Event

Microplastic Story talk session for Crowdfunding donnator25

2022-09-04 Event

Beach clean and Tara presentation @odawara

60

2022-09-24 Event

Tara Awashima Club with winner of Tara Poster Concours 2022

8

2022-09-29 Expo

Setouchi triennale 2022

2022-10-07 Event

Tara Presentation @lycee francais international de kyoto

302022-10-08 Event

Crowdfunding return event at Setouchi triennale 2022

1133

16 000

12130

Date

Event type

Event Description

Number of
Attendees

2022-10-22 Event

Tateyama Educational event for adult172022-10-23 Event

Tateyama Educational event for kids

25

2022-11-02 Event

Shimoda Sampling with Veolia, and donateur of crowdfunding

6

Tara Crowdfunding Reward seminar @agnesb-tara Office and2022-12-04 Event

4
Online2023-02-09 Presentation

Tara Presentation @CCIFJ

40

2023-03-25 Expo

TARA JAMBIO ART PROJECT Exhibition (as of March 31)

9334

Researchers using the Center
1) Main Research Topics of those Researchers
Research Subject

PI affiliation

#

Host

ゲノム編集ホヤの作製

サントリー⽣命科学財団

1

Sasakura

遺伝⼦破壊カタユウレイボヤの作製

サントリー⽣命科学財団

1

Sasakura

ウミホタルおよびその近縁種の採取

産業技術総合研究所

4

Sasakura

岩⼿⼤学⼤学院連合農学研 1

Sasakura

ホヤを⽤いた遺伝⼦実験
究科
海中ロボットの動作試験

東京⼤学⽣産技術研究所

20

Sasakura

海洋測器の操作訓練

⽇本海洋株式会社

12

Sasakura

海⽔や海底の電気特性測定、および電波伝搬測定

ソニーグループ株式会社

6

Sasakura

船上および岸壁からのROV（南極ブルー）の操作訓練 国⽴極地研究所

2

Sasakura

海⽔濾過槽に⽣息するキヌタレガイ（⼆枚⾙の⼀種）

2Sasakura

2

Sasakura

東京海洋⼤学を採集
カタユウレイボヤのゲノム編集に関わる実験技術を学
ぶため、マイクロインジェクションなどの技術指導を 岩⼿医科⼤学
受ける
ホヤ卵のゲノム編集

岩⼿医科⼤学1

Sasakura

つくばⅡによる海洋計測

海洋研究開発機構

3

Sasakura

1

Sasakura

沖縄科学技術⼤学院⼤学

1

Inaba

CNRS / Univ P et M Curie

1

Inaba

カブトクラゲの成体と幼⽣を使った分⼦⽣物学的実験 エクセター⼤学

1

Inaba

軟体動物の⽣殖メカニズム・戦略を調べるための実験 東北⼤学

1

Inaba

岩礁潮間帯性ヤドカリ類の系統地理学的研究に関わる
京都⼤学
採集
カブトクラゲを利⽤した共同研究

マイクロプラスチックに関する共同研究
Paris635

アユ⽣殖に関する共同研究

群⾺県⽔産試験場

1

Inaba
Inaba・

Keimyung University ·海洋酸性化に関する共同研究打ち合わせ

HallCollege of Natural Sciences
Spencer1

クシクラゲの平衡器官形成の共同研究

Inaba・

⽇本⼤学医学部
Shiba

科学研究費助成事業 学術変⾰領域研究（A）「ジオラ
マ環境で覚醒する原⽣知能を定式化する細胞⾏動⼒

9北海道⼤学 他

Inaba・
Shiba学」第8回 総括班会議（領域幹事会）
ジオラマ⾏動⼒学領域内研究交流会「多様な⽣殖細胞

7

Shiba

7

Shiba

3

Shiba

東北⼤学 他の運動様式」
海藻（Padina属）の採集

静岡⼤学

科学研究費助成事業 学術変⾰領域研究（A）「ジオラ
マ環境で覚醒する原⽣知能を定式化する細胞⾏動⼒

東北⼤学学」領域内共同研究
カジカ科⿂類の精巣発現タンパク質の同定

⼤阪公⽴⼤学1

Shiba

新規センサーを⽤いた海中及びホヤ精⼦の観察

海洋研究開発機構

3

Shiba

イメージング技術に関する研究打ち合わせ

埼⽟⼤学

1

Shiba

精⼦⾛化性に関する共同研究

東京⼤学

1

Shiba

ホヤ配偶⼦のサンプリング

静岡⼤学

1

Shiba

ヤッコカンザシの採集

筑波⼤学

1

Yaguchi

ウニ殻活⽤の共同研究打ち合わせ

JRE 洋上⾵⼒開発本部

2

Yaguchi

ウニ遺伝情報解析の共同研究

理化学研究所1

Yaguchi2

Yaguchi

リージョナルフィッシュ株 1

Yaguchi

海産動物飼育の共同研究（甲殻類幼⽣の飼育法とウニ
幼⿂⽔族館の飼育法の情報を組み合わせた飼育法の開発）

棘⽪動物ゲノム編集の共同研究
式会社
顕微鏡イベントにおける撮影技術の研究

36

NHK

3

Yaguchi

研究材料（フタツメイソウミグモ）の採集

新潟⼤学

1

Nakano

研究材料（海藻）のサンプリング

東京海洋⼤学5

Wada

バイブロコアラー試験およびROV試験

国⽴極地研究所

9Wada

海洋酸性化に関する研究打ち合わせ

東京海洋⼤学3

Wada

3

Agostini

底⽣動物群集に対する海洋酸性化の影響調査

東京海洋⼤学・Harvey

伊⾖周辺の藻場についての情報収集とブルーカーボン

1

Wada

2

Wada

2

Wada・

北海道⼤学
についての意⾒交換
タイワンホウキガニおよびクラゲムシ類の採集

北⾥⼤学

多波⻑励起蛍光カメラの試験

海洋研究開発機構
Agostini

国内9湖沼の溶存有機物化学形態の分析を⽬的とする蛍

4

Wada

東京⼤学
光性溶存有機物測定装置の利⽤
海洋⽣物の採取と⾼分⼦合成

筑波⼤学

2

Wada

海産⽣物からの有⽤物質の採取

筑波⼤学

1

Wada

7

Wada

環境省モニタリングサイト1000（沿岸域調査）に関す
三重⼤学
る藻場調査
Isotope Analysis using Mass Spectrometer

筑波⼤学

2

Wada

元素分析計-質量分析計の利⽤

筑波⼤学

1

Wada

観測測器（海中グライダー）の試験・操作習熟

気象研究所

4

Wada

気候変動に対する⿂類の応答解析

University of Adelaide

1

Wada

3

Wada

ミサゴの⽻サンプルについて、炭素および窒素の安定
筑波⼤学
同位体⽐の分析を⾏う(⾃然同位体⽐測定)
海⽔中に存在する糖鎖の測定

名古屋⼤学

2

Wada

海洋プラスチック採取

筑波⼤学

1

Wada

1

Agostini

7

Agostini

海洋酸性化による⿂類の⾏動異常を解明するプロジェ
沖縄科学技術⼤学院⼤学
クトへの参加
Tara-Jambioにおいてマイクロプラスチック調査・啓発 Tara Ocean Japan37

活動を⾏う
新たな材料へ海産⽣物の加⼊に関する研究

東京理科⼤学2

Agostini

マイクロプラスチックサンプリング

沖縄科学技術⼤学院⼤学

1

Harvey1Harvey3Harvey4

Porzio

式根島で収集した藻類のサンプルを利⽤し海洋酸性化
沖縄科学技術⼤学院⼤学の環境への影響を評価する実験を⾏う
海洋酸性化による⿂類の⾷事⽣活への影響を評価する University of Adelaide
Sathyabama Institute ofVisit to learn ocean acidification and its impact on
Science and Technology,
marine organisms
Chennai, INDIA

2) Research Output from Researchers using the Center
Hayakawa E, Guzman C, Horiguchi O, Kawano C, Shiraishi A, Mohri K, et al. Mass spectrometry of short
peptides reveals common features of metazoan peptidergic neurons. Nature Ecology & Evolution.
6(10):1438–48. August 2022.
Kushida Y, Imahara Y, Wee HB, Fernandez-Silva I, Fromont J, Gomez O, et al. Exploring the trends ofadaptation and evolution of sclerites with regards to habitat depth in sea pens. PeerJ. 10:e13929. September
2022.
Ito T, Morita M, Okuno S, Inaba K, Shiba K, Munehara H, et al. Fertilization modes and the evolution of sperm
characteristics in marine fishes: Paired comparisons of externally and internally fertilizing species. Ecology
and Evolution. 12(12): e9562. December 2022.
Sensui N, Itoh Y, Okura N, Shiba K, Baba SA, Inaba K, et al. Spawning-induced pH increase activates sperm
attraction and fertilization abilities in eggs of the Ascidian, Phallusia philippinensis and Ciona intestinalis.
International Journal of Molecular Sciences. 24(3):2666. January 2023.

38

Issued by
University of Tsukuba
Shimoda Marine Research Center〒415-0025 Shizuoka-ken, Shimoda-shi, 5-10-1
TEL : 0558-22-1317 / FAX : 0558-22-0346
URL: https://www.shimoda.tsukuba.ac.jp
E-mail: jim@ shimoda.tsukuba.ac.jp(Administration)

39