P-62
生命科学

プロヒドロジャスモンの小松菜の成長に対する作用メカニズム
SATテクノロジー・ショーケース 2024

■ はじめにProhydrojasmon (PDJ) is a plant growth regulator
analogous to jasmonic acid (JA) that has been used toregulate various aspects of growth and development in plants.
Our previous study has revealed that application of PDJ
induced total phenolic and anthocyanin content inkomatsuna (Azis et al., 2019). However, as a stress-related
hormone PDJ also possesses inhibitory effect on plant
growth (Azis et al., 2020). How far this effect and how themechanism of action behind it remain unclear. In this study,
PDJ effect on the growth of komatsuna and their possible
mechanism action was investigated.

PDJ also upregulated some pathogen-related genes (MLOs)
attributable to the premature leaf senescence. Furthermore,
nutrient recyle and cell-loosening related genes (BCAT4and EXPA) also upregulated by PDJ which also linked to thesenescence cascade.
３．Conclusion
Conclusively, PDJ at lower concentrations did not cause
inhibitory effect to the growth of komatsuna. However, at
higher concentrations, PDJ started to show significant
inhibitory effect indicated by wilting leaves. This inhibitory
effect can be explained by the change expression of some
genes involved in multiple senescence mechanisms.

■ 活動内容１．Experimental section
Komatsuna was grown in growth chamber at 23 ◦C and
60% relative humidity with a 14 h light/10 h dark photoperiod.
After two weeks, komatsuna was treated with PDJ at 2001000 ppm and their growth parameters was evaluated. Gene
expression analysis was performed to find out the possible
mechanism action of PDJ on the growth of komatsuna.
Komatsuna was grown at similar conditions and was treatedwith PDJ at 200 and 1000 ppm. Total RNA of komatsuna
leaves were extracted and small scale library was prepared.
RNA-seq data was generated and further analyzed.
２．Findings
2.1 PDJ at high concentration inhibit growth of komatsuna
PDJ did not show significant change in growth parameterof aerial part of komatsuna in all concentration. However,
PDJ at higher concentration showed growth reduction
tendency, particularly PDJ spray at 1000 ppm caused wilting
leaves in komatsuna which may indicate senescence. PDJ at
600 and 1000 ppm also significantly reduced the length andweight of root (Figure 1).
2.2 PDJ altered gene expression involved in senescence
Gene expression analysis revealed that PDJ at 1000 ppm
induced a significant change in the expression of several
genes linked to senescence (Figure 2). Some genes fromchlorophyll cluster genes (ATPB, LHCBs, CHL, HCFP244)
were found to be downregulated in leaves treated with PDJ.
Subsequently, increase expression of some senescenceassociated genes (SAGs) was also observed. Additionally,■ 関連情報等（特許関係、施設）
1. Azis et al., 2019. Biosci, Biotech, & Biochem 84: 178–186.
2. Azis et al., 2020. Plants (Basel) 9: 1368

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Figure 1. length of root (left) and weight of root (right)

Figure 2. Mechanism action of PDJ on growth of komatsuna■キーワード： （1）プロヒドロジャスモン（2）小松菜（3）植物の成長■共同研究者： 高橋真哉 1, 佐野友春 2, 青野光子 3, 中嶋信美 3,
腰山雅巳 5, 藤澤浩 5, 富永健一 4, 礒田博子 1,4〒305-8572 茨城県つくば市天王台 1 丁目 1-1
1． 筑波大学・生命環境
2． 国環研・リ・環共同研究棟 A312
3． 国環研・生物、
TEL：029-853-7871
4． 産総研・FoodMed-OIL
haidarrafid.azis@aist.go.jp
5． 日本ゼオン‒ 74 ‒

Haidar Rafid Azis（ハイダル ラフィド アジス）
産業技術総合研究所
食薬資源工学
オープンイノベーションラボラトリー