Ion S5™/PGM™

Run PCR with a
LymphoTrack Dx
Assay

Purify amplicons

Quantify& preparelibrary

PrepareDNAsamples

Unparalleledworkflow simplicity
LymphoTrack Dx Assays put lg/ TCR
next-generation sequencing at your fingertips.

Load & run
Ion S5TM/PGMTM

Analyze

Process datawith included
bioinformatics

These are in vitro diagnostic products, and are not available for sale or use within North America.

www.invivoscribe.com

Invivoscribe
Tel +1 858.224.6600 | Fax +1 858.224.6601
sales@invivoscribe.com
10222 Barnes Canyon Rd., Bldg. 1
San Diego, CA 92121 | USA

Invivoscribe, SARL
Tel +33 (0)4.42.01.78.10 | Fax +33 (0)4.88.56.22.89
sales-eu@invivoscribe.com
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Ion S5™/PGM™

Workflow Summary07
the PCR products using the Agencourt AMPure XP
08 Purify
PCR Purification system. Add 90 µL of particles to each01	Remove the amplification plate from the thermocycler.
®

	02

®

50 µL reaction; elute DNA in 40 µL TE buffer.

09	Quantify amplicons with an appropriate method(e.g. 2100 Bioanalyzer® or LabChip® GX).

03	the library by combining an equal amount of each10 	Create
amplicon in a tube (do not include the no template control).

04

11 Dilute the library.	12
the S5 or PGM. Load S5 [Ion 520, Ion 530] or PGM
13 	Initialize
chip [316 v2 BC, 318 v2 BC].

05	Prepare templates using the OT2 or Ion Chef system.

06
StepTemperature

TimeCycle

195 C

7 minutes

1o2o

95 C

45 seconds

360 oC

45 seconds

4o72 C90 seconds

5o72 C

10 minutes

1615 C∞

1o14 	Create a Planned Run using the Torrent Browser.
15 Start the Ion S5 or Ion PGM run.
and visualize the acquired data using the16 	Analyze
LymphoTrack Dx Software for the Ion S5/PGM.

29x
Storage Conditions: -85 °C to -65 °C (DNA controls may beseparated from kits and stored at 2 °C to 8 °C).
NOTE: Always follow the applicable instructions for use (IFU) when
testing samples.

Ordering Information
CATALOG #PRODUCTS

QUANTITY

9-121-0057

LymphoTrack Dx IGH FR1/2/3 Assay - S5/PGM™

12 + 12 + 12 indices - 5 sequencing reactions each

9-121-0007

LymphoTrack Dx IGH FR1 Assay - S5/PGM™

12 indices - 5 sequencing reactions each

9-121-0037

LymphoTrack® Dx IGH FR2 Assay - S5/PGM™

12 indices - 5 sequencing reactions each

9-121-0047

LymphoTrack Dx IGH FR3 Assay - S5/PGM™

12 indices - 5 sequencing reactions each

9-122-0007

LymphoTrack Dx IGK Assay - S5/PGM™

12 indices - 5 sequencing reactions each

9-227-0007

LymphoTrack® Dx TRG Assay - S5/PGM™

12 indices - 5 sequencing reactions each

9-500-0007

LymphoTrack Dx Software - S5/PGM™

1 CD complimentary with purchase®
®

®
®

®

These are in vitro diagnostic products, and are not available for sale or use within North America.
©2019 Invivoscribe, Inc. (Invivoscribe®). All rights reserved. The trademarks mentioned herein are the property of Invivoscribe and/or its affiliates, or (as to the trademarksof others used herein) their respective owners. Ion S5TM and Ion PGM™, HI-Q™, ONETOUCH™, ION CHEFTM, ION 316TM, ION 318TM, ION 510TM, ION 520TM and ION 530TM are
trademarks of Thermo Fisher Scientific or its subsidiaries. AGILENT® and BIOANALYZER® are registered trademarks of Agilent Technologies, Inc. AGENCOURT® is a registeredtrademark of Beckman Coulter, Inc. LABCHIP® is a registered trademark of Perkin Elmer.

016 Rev.G Jan.19 280357