E-Cadherin Antibody / CDH1 [clone CDH1/1525] (V3190)
Catalog No.

Formulation

Size

V3190-100UG

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

100 ug

V3190-20UG

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

20 ug

V3190SAF-100UG

1 mg/ml in 1X PBS; BSA free, sodium azide free

100 ug

Bulk quote request
Availability

1-3 business days

Species Reactivity Human
FormatPurified

Clonality

Monoclonal (mouse origin)

Isotype

Mouse IgG1, kappa

Clone Name

CDH1/1525

PurityProtein G affinity chromatography

UniProt

P12830Localization

Cytoplasmic, membranous

Applications

Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Limitations

This E-Cadherin antibody is available for research use only.

Formalin-fixed, paraffin-embedded human colon
carcinoma stained with E-Cadherin antibody(CDH1/1525).

Formalin-fixed, paraffin-embedded human colon
carcinoma stained with E-Cadherin antibody(CDH1/1525).

Formalin-fixed, paraffin-embedded human prostate
carcinoma stained with E-Cadherin antibody(CDH1/1525).

Formalin-fixed, paraffin-embedded human skin stainedwith E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human placenta
stained with E-Cadherin antibody (CDH1/1525).

Western blot analysis A) partial recombinant protein B)
human stomach lysate using E-Cadherin antibody(CDH1/1525). Expected molecular weight: 135 kDa(precursor), 80-120 kDa (mature, depending on
gylcosylation level).

Immunofluorescent staining of human MCF7 cells withE-Cadherin antibody (clone CDH1/1525, green) andReddot nuclear stain (red).

Analysis of HuProt(TM) microarray containing morethan 19,000 full-length human proteins using
E-Cadherin antibody (clone CDH1/1525). These results
demonstrate the foremost specificity of the CDH1/1525
mAb.
Z- and S- score: The Z-score represents the strength ofa signal that an antibody (in combination with afluorescently-tagged anti-IgG secondary Ab) produces
when binding to a particular protein on the HuProt(TM)array. Z-scores are described in units of standard
deviations (SD's) above the mean value of all signals
generated on that array. If the targets on theHuProt(TM) are arranged in descending order of theZ-score, the S-score is the difference (also in units ofSD's) between the Z-scores. The S-score therefore
represents the relative target specificity of an Ab to its
intended target.

SDS-PAGE analysis of purified, BSA-free E-Cadherin
antibody (clone CDH1/1525) as confirmation ofintegrity and purity.

Formalin-fixed, paraffin-embedded human breast
adenocarcinoma stained with E-Cadherin antibody(CDH1/1525).

Description
Recognizes a protein of 80-120kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion
molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The
classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of fivehomologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins,
such as Î²-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased
expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate andesophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) andlobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Application Notes
Optimal dilution of the E-Cadherin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min
followed by cooling at RT for 20 min.

ImmunogenA full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.

Storage
Store the E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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