Histone Antibody / Histone H1 [clone 1415-1] (V2567)
Catalog No.

Formulation

Size

V2567-100UG

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

100 ug

V2567-20UG

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

20 ug

V2567SAF-100UG

1 mg/ml in 1X PBS; BSA free, sodium azide free

100 ug

V2567IHC-7ML

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC
use only*

7 ml

Citations (4)Availability

Bulk quote request

1-3 business days

Species Reactivity Human, Mouse, Rat
FormatPurified

Clonality

Monoclonal (mouse origin)

Isotype

Mouse IgG2a, kappa

Clone Name

1415-1

PurityProtein G affinity chromatography

UniProt

P07305

Localization

NuclearApplications

Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western blot : 1-2ug/ml

Limitations

This Histone antibody is available for research use only.

IHC: Formalin-fixed, paraffin-embedded human tonsil
stained with Histone antibody (clone 1415-1).

IHC: Formalin-fixed, paraffin-embedded human tonsil
stained with Histone antibody (clone 1415-1).

IHC: FFPE human testicular carcinoma tested with
Histone H1 antibody (clone 1415-1).

IHC: FFPE human pancreas tested with Histone H1antibody (clone 1415-1).

IHC: FFPE human ovarian carcinoma tested with
Histone H1 antibody (clone 1415-1).

IHC: FFPE human angiosarcoma tested with Histone H1
antibody (clone 1415-1).

Western blot testing of human heart lysate with
Histone antibody (clone 1415-1). Observed molecularweight ~22/30-33kDa (unmodified/modified).

Immunofluorescent staining of fixed human HeLa cellswith Histone antibody (clone 1415-1, green) andPhalloidin (red).

Flow cytometry testing of fixed human HeLa cells withHistone antibody (clone 1415-1); Red=isotype control,
Blue= Histone antibody.

SDS-PAGE analysis of purified, BSA-free Histone
antibody (clone 1415-1) as confirmation of integrityand purity.

Description
Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles bywrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two moleculesof each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4
dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1,
derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher
order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, butalso in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation,sumoylation, ubiquitination and ADP-ribosylation.

Application Notes
Optimal dilution of the Histone antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followedby cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (ifrequired), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. View the recombinant form of this Histone H1 antibody.

ImmunogenNuclei of human leukemia biopsy cells were used as the immunogen for the Histone H1 antibody.

Storage
Store the Histone antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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