THE CYTOF ADVANTAGE
Reasons to take your high-dimensional research beyond the limits of fluorescence1

Not impacted by spectral overlapping offluorochromes and tissue autofluorescence

89Y

Y (1)
Pd (6)
Rh (1)

24

Precision data with discrete signals

110Pd

Cd (7)I (1)
Pr (1)141Pr

Nd (7)
Sm (4)
Eu (2)150Nd

Gd (4)Tb (1)Dy (4)

161Dy

Ho (1)Er (4)Tm (1)

191lr

209Bi

Yb (5)Lu (1)Ir (2)

Pt (4)Bi (1)

Unbiased, high-dimensional profilingof 40-plus markers to uncover diverse
immune subpopulations

Large number of available antibodies without overlap
simplifies panel design and expansion

Reproducible and comparable
Stained samples can be frozen, stored andshipped to support longitudinal studies
and multi-site workflows

Cytometry bytime-of-flight(CyTOF® technology)
Applies purified heavy-metallabels, not normally found in
biological systems, instead offluorophores

Risks of fluorescence

Easy panel design to complete experiments fasterStart with ready-to-go
panels and easily swap
markers in and out

3Capture rare or unexpected cell populations

for high-parameter studies

56

Minimal sample required, saving on
limited clinical research material
Simultaneous staining
and detection from asingle tube or tissue
scan, without multiple
staining controls ortime-consuming cyclic
protocols

Trusted by researchers
The leading technology
for high-parameter
immune researchMissed cell
populations or
false positives
More iterations
required in panel
designReduced sensitivity
where fluorescenceoverlap occurs>2,000publications>200
clinicaltrialsHigher resource use
to compensate forspectral overlap

From sample collection to high-dimensional insights in 3 days*
Whether you are analyzing suspension or tissue samples, time-of-flight (TOF) technology combined with Maxpar® reagents enables a
streamlined end-to-end workflow to complete high-parameter experiments faster than fluorescence-based detection.

Flow cytometry

Imaging Mass Cytometry

Get started with the validated Maxpar®
Direct™ Immune Profiling Assay™

Get started with our Maxpar IMC™ Cell Segmentation
Kits and ready-to-go high-plex panels

1 tube 30 markers(<300 µL of whole blood)

5-minute analysis

37 cell populations

Simultaneous staining

One-step detectionSurface profiles
Cell proliferation
Apoptosis
Metabolism
Phosphoproteins
Cytokine production
Transcription factors

High-plex data in minutes
Tissue architecture
Protein modifications
Signaling pathway activation
Cell injury states
Cell proliferation
Transcriptional signatures

Clear spatial imaging

Minimal signal overlap
The CyTOF flow
cytometry image (farleft) shows minimal
spillover between
metal channels whencompared with thesame panel from acompetitor spectral
flow cytometer (left).

The Imaging Mass Cytometry™
image (far left) shows many welldefined red signals from CD68
that are indistinct or missing fromthe fluorescence image (left).

Quantify and visualize 40-plus markers in a single run. Without compromise.
*After panel and image analysis optimization

For Research Use Only. Not for use in diagnostic procedures.
Information in this publication is subject to change without notice. Limited Use Label License: The purchase of this Standard BioTools Instrument and/or Consumable conveys to the purchaser the limited, nontransferable right to use only with Standard BioTools Consumables and/or Instruments respectively except as approved in writingby Standard BioTools Inc. (f.k.a. Fluidigm Corporation): www.standardbio.com/legal/salesterms. Patents: www.standardbio.com/legal/notices. Trademarks: Standard BioTools, the Standard BioTools logo, Fluidigm, the Fluidigm logo, CyTOF, Direct, Imaging Mass Cytometry, IMC, Immune Profiling Assay and Maxpar are trademarks and/orregistered trademarks of Standard BioTools Inc. or its affiliates in the United States and/or other countries. ©2023 Standard BioTools Inc. All rights reserved. 04/2023