Flow Cytometers for Research and Industry

CyFlow Cube Series

Reliable, Compact andEconomical Flow
Cytometers withGreat Performancewww.sysmex-ap.com

CyFlow Cube 6 and Cube 8Compact, Economic Flow Cytometerswith Exceptional Performance
Flow cytometry (FCM) is no longer limited tohigh-end cell biology laboratories or core researchfacilities. When performing basic research and/or industrial
monitoring, you need access to easy and cost-eﬀective FCM
technology that will help you advance your research.
Standard applications and beyond are now within yourreach, such as:. Cell Counting. Viability testing. Ability to determine apoptosis. Fluorescent protein expression. Immunophenotyping
The CyFlow Cube series, CyFlow Cube 6 and the CyFlow
Cube 8 instruments are fully integrated, compact, robust
FCM solutions that will meet your daily research laboratoryneeds. These innovative, stand-alone flow cytometers oﬀer
all you need to precisely acquire cells or particles fromsamples in a powerful single device.
A network-ready computer, a foldable LCD monitor, andstate of the art software make the CyFlow series thecomplete research solution. FCM technology integrated in
an award-winning product design.
*(CyFlow® Cube 8)(CyFlow® Cube 6)

Cyflow Cube Series

Economic viability is crucial to your research and the advancement ofscience. Why sacrifice quality due to budget constraints? The CyFlow
Cube series provides the customisable high level of technology thatyou need at a low operating cost.

Fluorescence-based flow cytometry (FCM) was invented by Partec in 1968. Today
Sysmex can look back on over 50 years’ experience in this demanding field. We
continue to develop and manufacture our own technologies and instruments, withalmost 80 % created in-house.
As a result, Sysmex stands for high precision and quality ‘Made in Germany’. Our
technology and extensive knowledge are appreciated in various fields, such as basic
research, clinical research, industrial applications and disease monitoring.

CyFlow Cube 6This cost-eﬀective system is our most compact flow cytometer.
It comes in three basic configurations with the most commonlyused lasers, blue 488 nm and red 640 nm, and with up to six
optical parameters. You can expand this space-saving instrumentwith an autoloading station.

Cyflow Cube Series

Quality through experience
Our instruments provide premium technology with high stability,sensitivity and resolution. The stability of the optical bench leads toa virtually calibration-free system with no need for daily functionalverification. Its precision blends in excellently with the accurate
fluidic system in variable configurations.
This high standard of the optical bench is complemented byelectronics and a computer system to match that deliver
the basis for real-time signal analysis and processing with highfluorescence and scatter sensitivity.

CyFlow Cube 8This model is more flexible and modular. You can configure it individually by choosing from ten
diﬀerent light sources and numerous optical filter sets. You can combine up to three lasers anda UV LED with up to eight optical parameters in your system. You can expand your CyFlow Cube 8with an autoloading station and/or integrate a piezo-electric cell sorter module.

Take your Research from Basic Application
to an Optimised Solution.
The modular architecture of the Sysmex CyFlow Cube instruments oﬀers you a variable choiceof configurations to match your applications. Customise your device now, and then adapt it foryour next project. You can select from a portfolio of the most recent lasers, optical parameters,
an automated sample loading device and even a sorter module (Cube 8). This technological advantage paired with brand new software lets you adapt your instrument to suit most applicationsin industry and research.
CyFlow Robby Autoloading Stations

The cell sorter module is one of Sysmex’s unique technicalsolutions. As an optional add-on for the CyFlow Cube 8 it

combines a flow chamber with a piezo element, including
electric activation. This cell sorter lets you sort cells orparticles stably and non-destructively with high yield andpurity. It works as a closed system and, in contrast to typical
droplet sorters, the process is smooth with reduced
mechanical stress. This is important when working withfragile cell types, such as neuronal stem cells. As a closed
sorting solution, it is aerosol-free so you avoid biohazardous exposure yet lets you deliver sterile sorting ofviable cells for subsequent cell culture. For operator safety
you can place your CyFlow Cube 8 Sorter under a standard
clean bench – this is possible thanks to its small footprint
with no external components (see page 8).

CyFlow Cube 6 with Robby 6 Autoloading Station

CyFlow Cube 8 with Robby 8 Autoloading Station

There are two diﬀerent models available. The Robby 6 andRobby 8 complement the Cube 6 and Cube 8 instruments
to which they are directly connected. Our autoloading
stations are controlled by the CyFlow Software and let
you quickly load your flow cytometer – automaticallyand accurately. Both stations can process two standard
and deep-well 96-well-plates or 48-well-plates (with V, Uand flatbottom) and up to 120 tubes from a single load.

CyFlow Cube Sorter Module

Cyflow Cube Series

Operational Simplicity Supports your Workflow
CyFlow Cube instruments impress with their accessibility, simplicity and intuitive software operation. And thanks toits automated start-up process it takes less than five minutes to start up and you can get to work in the mornings prettymuch straight away.
CyFlow Software, the Cube series’ software, provides state-of-the-art software functionalities as all-in-one software foracquisition and analysis. It is intuitive, easy-to-use and highly flexible for use with various applications. This helps you
save valuable time in your laboratory. CyFlow Software supports four analysis technologies: a pre-selected event
limit in regions, syringe-controlled volumetric measurement, a time-controlled measurement and the special‘True Volumetric Absolute Counting’ (TVAC) – Sysmex’s unique counting principle that requires no time-consuming andcost-intensive reference counting beads.
With CyFlow, you get an easy-to-learn and convenient tool for data acquisition and analysis in a single package.
It is a highly adaptable and flexible operating tool for each application. Its many state-of-the-art functionalities provideinstrument control and acquisition, on- and oﬄine data analysis and safe data management. You can use CyFlow formost applications in cell biology, especially in combination with the excellent Sysmex monoclonal antibody reagents,
biotechnology and microbiology, and in industrial monitoring processes, such as beverage control.

As flexible as can be
This brand-new software includes hierarchical and logical gating.
Wizards for cleaning procedures, spillover-compensation,
histogram or dotplot overlay are included. It also
oﬀers a report wizard including statistics,
compensation matrix and most editing
options. DNA analysis including sub-G
and Debris is provided by CyFlow Software.
It provides saving options for instrument settings,
gating strategies, entire work lists of up to 400
samples and much more. Acquisition data is
file
automatically saved in the current
format. Thanks to this unique combination offeatures, you are as flexible as can be to focus
on your research results.

Technical specifications
Lasers / LEDs

Detectors

BLUE LASER/

Exemplary dyes

Green
OrangeOrange Red
Red I
Red IIFar Red

RED LASERRed I
Red IIFar RedVIOLET LASERBlueGreenOrange

UV LASERBlue

Available for

FITC / GFP / Alexa Fluor
PE
PE-Texas Red / PI

Pacific Blue™ / Alexa Fluor®

HIGH-POWER UV LED
GREEN LASER/OrangeRed

mStrawberry / PE
mCherry / PI / PE-Texas Red®

YELLOW LASER

OrangeRedPE / DS Red / PE-Texas Red®

ORANGE LASER

Orange RedRed
Far RedTexas Red® / Alexa Fluor®
APC / mCherrymPlum

Available light sources and exemplary detector configurations (various filters available)

Light sources and optics
n CyFlow Cube 6:
optical parameters: max. 6 (4 colours + FSC + SSC)
light sources: max. 2 lasers (488 nm and 638 nm or 640 nm)
n CyFlow Cube 8:
optical parameters: max. 8 (6 colours + FSC + SSC)
light sources: max. 3 lasers plus UV LED
n CyFlow Cube Sorter (Cube 8 with integrated sorter module):
optical parameters: max. 5 (3 colours + FSC + SSC),
light sources: max. 2 lasers
n optical parameters with selected PMTs and exchangeable optical filters
Flow system
Quartz flow cuvette for laminar sample transport and hydrodynamic
focusing with sheath fluidn Sample port with biosafety cleaning systemn True Volumetric Absolute Counting (TVAC) based on mechanicalvolume measurementn Computer-controlled syringe pump speed, continuously adjustablefrom 0.1 – 20 µL/sec and three fixed speed settings (Low 0.5 µL/sec,Medium 2 µL/sec, High 5 µL/sec)n Cube 6 2x bottles (external), Cube 8 2x bottles (built-in with drawer)
without external components
n Syringe-based silent operationnElectronics and signal processing
Selectable linear or 4-decade logarithmic scalen 16-bit analogue-to-digital converters, selectable trigger parameter(with ‘and/or’ logic) and individual threshold level settings
n V-Log (providing access to negative value dispaly)nSoftwaren Operating system: Microsoft Windows™
n CyFlow Software for data acquisition, analysis and reporting
Computer system
Integrated Microsoft Windows™ PC and network capabilityn Integrated, foldable colour LCD TFT display (15" Cube 6/19" Cube 8)
n 4x USB port, 2x Display-port, 1x VGA port, 1x Ethernet port
nOptions
CyFlow Robby 6 Autoloading Station for CyFlow Cube 6,
CyFlow Robby 8 Autoloading Station for CyFlow Cube 8n CyFlow Cube Sorter module for CyFlow Cube 8nWeightCube 6: 18 kg; Cube 8: 40 kg

nDimensions (WxHxD)
n Cube 6: 385 x 290 x 280 mm; with open display 528 mm heightn Cube 8: 500 x 370 x 470 mm; with open display 670 mm height

Design and specifications may be subject to change due to further product development.

Instrument models
n CyFlow Cube 6, CyFlow Cube 8

SAP/FlowCytometry/CyFlowCube/28022019/V1.2