Selection Guide

Agilent AssayMAP Bravo Cartridges
for Automated Protein Sample
Preparation Workflows

5 µL

25 µL

Introduction
The Agilent AssayMAP platform is a powerful yet easy-to-use automation
solution specifically designed for biomolecule sample preparation. AssayMAP
microchromatography cartridges combined with optimized protocols enable
non‑automation experts to use automation to increase precision and scalability
while at the same time decreasing the labor and human error so you havemore confidence in your results and more time to analyze your biomolecule.
The AssayMAP platform supports a broad range of protein quantification andcharacterization workflows including affinity purification, enzymatic digestion,
protein and peptide cleanup, peptide mapping, peptide fractionation, N-Glycan
analysis, and phosphopeptide enrichment.

Cartridge types
Resin Bed Volume
ProductApplication

5 µL

25 µL
G5496-60017

C18Desalting mixtures of peptides and small proteins prior to analysis by mass spectrometry.

5190-6532

Fe(III)-NTA

Phosphopeptide enrichment prior to analysis by mass spectrometry.

G5496-60085–Protein A (PA-W)

Purification of monoclonal and polyclonal antibodies and Fc fusion proteins with affinity for Protein A.

G5496-60000

G5496-60018

Protein G (PG-W)

Purification of monoclonal and polyclonal antibodies and Fc fusion proteins with affinity for Protein G.

G5496-60008–Resin-Free

Placeholder when working with sample numbers that are not a multiple of eight.

G5496-60009–

Reversed Phase (RP-S)

Desalting or fractionating mixtures of peptides and small proteins prior to analysis by mass
spectrometry.

G5496-60033

G5496-60023

Reverse Phase (RP-W)

Desalting of denatured proteins (antibodies) prior to trypsin digestion.

G5496-60086–

Streptavadin (SA-W)

Generation of affinity cartridges by immobilizing biotinylated molecules. Affinity cartridges cansubsequently be used to purify target molecules from complex matrices.

G5496-60010

G5496-60021

Strong Cation Exchange (SCX)

Fractionating mixtures of peptides and small proteins prior to analysis by mass spectrometry.

5190-6536–

TiO2

Phosphopeptide enrichment prior to analysis by mass spectrometry.

G5496-60016–

Starter kits (including cartridges and required labware)
ProductDescriptionAssayMAP Digestion andC18 Cleanup Starter Kit

1 rack of C18 cartridges
plus labware for digestionand cleanup protocols

Application

Catalog Number

Protein digestion and subsequent peptide cleanup prior to analysis by mass spectrometry.

G5496-60013

AssayMAP Digestion andRP-S Cleanup Starter Kit

1 rack of RP-S cartridges
plus labware for digestionand cleanup protocols

Protein digestion and subsequent peptide cleanup prior to analysis by mass spectrometry.

G5496-60034

AssayMAP SCX
Fractionation Starter Kit

1 rack of SCX cartridges
plus labware for digestionand cleanup protocols

Protein digestion and subsequent peptide fractionation prior to analysis by mass spectrometry.

G5496-60014

Supplemental items
ProductDescription

Application

Catalog Number

Syringe Test Kit

96 resin free cartridges (1 rack plus enough syringe test solution toperform 20 tests)

Routine verification that all 96 the AssayMAP
syringes.

G5496-60050

Syringe Replacement Kit

10 replacement syringes plus the tools required to replace faulty
syringes.

Allows customers to replace the syringes onsite to avoid costly down time.

G5409-68002

AssayMAP Syringes

10 AssayMAP syringes

AssayMAP syringes for use with the syringe
replacement kit.

G5496-60007

Bravo Plate Risers

1 Plate Riser

Accessory Required for normalization,serial dilution, and reformatting utilities.

G5498B#061

Bravo Closed Corner Plate Pad

Plate pad designed to minimize the risk of plate misalignment.

Alternative for standard Bravo plate pads.

G5498B#125

PCR Plate Thermal Insert

Thermal insert for the Peltier heater/cooler to improve heat transfer
to the plate wells.

For In-Solution Digestion Single plate andOn‑Cartridge Reaction applications.

G5498B#013

96 well Greiner U,V-Bottom
Plate Thermal Insert

Thermal insert for the Peltier heater/cooler to improve heat transfer
to the plate wells.

For In-Solution Digestion Single plate and
On‑Cartridge Reaction applications.

G5498B#126

96 well Abgene Deep Well
Plate Thermal Insert

Thermal insert for the Peltier heater/cooler to improve heat transfer
to the plate wells.

For In-Solution Digestion Single plate and
On‑Cartridge Reaction applications.

G5498B#127

Wide-Bore Chimneys

100 pack wide-bore chimneys

25 µL cartridges require wash station to beequipped with wide-bore chimneys*G5409-68004* For instructions on upgrading, see the 96 Channel Wash Station Maintenance Guide on Agilent.com

2

Additional cartridge details
Cartridge
C18 5 µL
Part Number5190-6532

Bead TypeSilica (20 µm diameter)

Pore Size150 Å

Surface Chemistry

C18 (reversed-phase)

Dynamic Binding Capacity

400 µg of human insulin (≤10% breakthrough when loading up to 400 µg of human insulin per cartridge)
Approximately 150 to 200 µg of digested BSA (loading more than 150 µg of digested BSA results in loss of veryhydrophilic peptides)

Saturation Binding CapacityApproximately 450 µg of human insulin

Storage Temperature

Room temperature

pH Stability

2 to 7 for routine use

Part Number

G5496-60017

Bead TypeSilica (20 μm diameter)

Pore Size150 Å

Surface Chemistry

C18 (reversed-phase)

Dynamic Binding Capacity

1,800 μg of human insulin (≤10% breakthrough when loading up to 1,800 μg of human insulin per cartridge)
Approximately 700 to 900 μg of digested BSA (loading more than 700 μg of digested BSA results in loss of veryhydrophilic peptides)

Saturation Binding Capacity

Approximately 2,000 μg of human insulinStorage Temperature

Room temperature

pH Stability

2 to 7 for routine use

Part Number

G5496-60085

Bead Type

Synthetic Polymer (50 µm diameter)

Pore Size

Large enough for large biomolecules

Surface Chemistry

Fe(III) chelated to nitrilotriacetic acid

Dynamic Binding Capacity

20 µg of adenosine monophosphate (≥ 90% recovery when 20 µg of adenosine monophosphate is loaded per cartridge)
Up to approximately 800 µg of digested yeast extract can be loaded per cartridge with minimal phosphopeptide
breakthroughSaturation Binding Capacity

Approximately 25 µg of adenosine monophosphate

Storage Temperature

Room temperature

pH Stability

2 to 11 (cartridge will turn more yellow/gold with increasing pH)

Part Number

G5496-60000

Bead Type

Synthetic polymer (53 to 61 µm diameter)

Pore Size

Large enough for large biomolecules such as antibodies

Surface Chemistry

Recombinant Protein A

Dynamic Binding Capacity

100 µg of human IgG1 (≤10% breakthrough when loading up to 100 µg of human IgG1 per cartridge)

Saturation Binding Capacity

Approximately 125 µg of hIgG1

Storage Temperature

Room temperature

pH Stability

6 to 8 for routine use, 3 to 11 for short exposure

Part Number

G5496-60018

Bead Type

Synthetic polymer (53 to 61 µm diameter)

Pore Size

Large enough for large biomolecules such as antibodies

Surface Chemistry

Recombinant Protein A

Dynamic Binding Capacity

800 µg of human IgG1 (10% breakthrough when loading up to 800 µg of human IgG1 per cartridge)

Saturation Binding Capacity

Approximately 1,000 µg of hlgG1

Storage Temperature

Room temperature

pH Stability

6 to 8 for routine use, 3 to 11 for short exposure

C18 25 µL

Fe(III)-NTA

PA-W 5 µL

PA-W 25 µL3PG-W
Part Number

G5496-60008

Bead Type

Synthetic polymer (45 to 53 µm diameter)

Pore Size

Large enough for large biomolecules such as antibodies

Surface Chemistry

Recombinant Protein G

Dynamic Binding Capacity

100 µg human IgG (≤10% breakthrough when loading up to 100 µg of human IgG per cartridge)

Saturation Binding Capacity

Approximately 150 µg of IgG

Storage Temperature

Room temperature

pH Stability

6 to 8 for routine use, 3 to 11 for short exposure

Part Number

G5496-60009

Bead TypeNA

Pore Size

NASurface ChemistryNA

Dynamic Binding Capacity

NA

Saturation Binding Capacity

NAStorage Temperature

Room temperature

pH Stability

NAPart Number

G5496-60033

Bead Type

Synthetic polymer (15 to 20 µm diameter)

Pore Size

100 Å

Surface Chemistry

Underivitized polystyrene-divinylbenzene (reversed-phase)

Dynamic Binding Capacity

400 µg of human insulin (≤10% breakthrough when loading up to 400 µg of human insulin per cartridge)
Approximately 150 to 200 µg of digested BSA (loading more than 150 µg of digested BSA results in loss of veryhydrophilic peptides)

Saturation Binding Capacity

Approximately 500 µg of human insulin

Storage Temperature

Room temperature

pH Stability

1 to 14

Part Number

G5496-60023

Bead Type

Synthetic polymer (15 to 20 μm diameter)

Pore Size

100 Å

Surface Chemistry

Underivitized polystyrene-divinylbenzene (reversed-phase)

Dynamic Binding Capacity

2,300 μg of human insulin (≤10% breakthrough when loading up to 2,200 μg of human insulin per cartridge)
Approximately 850 to 1,100 μg of digested BSA (loading more than 850 μg of digested BSA results in loss of veryhydrophilic peptides)

Saturation Binding Capacity

Approximately 2,300 μg of human insulin

Storage Temperature

Room temperature

pH Stability

1 to 14

Part Number

G5496-60086

Bead Type

Synthetic polymer (30 µm diameter)

Pore Size

Large enough for large biomolecules such as antibodies

Surface Chemistry

Underivitized polystyrene-divinylbenzene (reversed-phase)

Dynamic Binding Capacity

75 µg of denatured human IgG1 (≤10% breakthrough when loading up to 75 µg of denatured human IgG1 per cartridge)

Saturation Binding Capacity

Approximately 90 µg of denatured human IgG1

Storage Temperature

Room temperature

pH Stability

1 to 14Resin Free

RP-S 5 µL

RP-S 25 µL

RP-W4

SA-W 5 µL
Part Number

G5496-60010

Bead Type

Synthetic polymer (45 to 53 µm diameter)

Pore Size

Large enough for large biomolecules such as antibodies

Surface Chemistry

Recombinant Streptavidin

Dynamic Binding Capacity

50 µg of biotinylated BSA (≤10% breakthrough when loading up to 50 µg of biotinylated BSA per cartridge)
Approximately 90 µg of biotinylated IgG

Saturation Binding Capacity

Approximately 60 µg of biotinylated BSA
Approximately 100 µg of biotinylated IgG

Storage Temperature

4 to 10 °C

pH Stability

6 to 8 for routine use, 3 to 11 for short exposure

Part Number

G5496-60021

Bead Type

Synthetic polymer (45 to 53 µm diameter)

Pore Size

Large enough for large biomolecules such as antibodies

Surface Chemistry

Recombinant Streptavidin

Dynamic Binding Capacity

350 μg of biotinylated BSA (≤10% breakthrough when loading up to 350 μg biotinylated BSA per cartridge)

Saturation Binding Capacity

Approximately 450 μg of biotinylated BSA

Storage Temperature

4 to 10 °C

pH Stability

6 to 8 for routine use, 3 to 11 for short exposure

Part Number

5190-6536

Bead TypeSilica (20 µm diameter)

Pore Size

300 Å

Surface Chemistry

Polysulfoethyl A (strong cation exchange)

Dynamic Binding Capacity

800 µg of human insulin (≤10% breakthrough when loading up to 800 µg of human insulin per cartridge)
Approximately 400 to 500 µg of digested BSA

Saturation Binding Capacity

Approximately 1,000 µg human insulin

Storage Temperature

Room temperature

pH Stability

2 to 7 for routine use

Part Number

G5496-60016

Bead Type

Ceramic titanium dioxide (20 µm diameter)

Pore Size

100 Å

Surface Chemistry

Titanium dioxideDynamic Binding Capacity

80 µg of phenyl phosphate (≥ 90% recovery when 80 µg of phenyl phosphate is loaded per cartridge)
Up to approximately 1,000 µg of digested α-casein can be loaded per cartridge with minimal phosphopeptide
breakthroughSaturation Binding Capacity

Approximately 115 µg of phenyl phosphate

Storage Temperature

Room temperature

pH Stability

1 to 14

SA-W 25 µL

SCXTiO2

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For Research Use Only. Not for use in diagnostic procedures.
DE.2819444444
This information is subject to change without notice.
© Agilent Technologies, Inc. 2015, 2016, 2019, 2020, 2021
Printed in the USA, September 23, 2021
5991-4863EN