Focused power.
Proven data quality.
The MiSeq® Desktop Sequencing System. Speed and simplicity
for targeted resequencing and small-genome sequencing.

Experience world-class data
accuracy and ultra-simplicity.
Easy workflow. Extensive applications.
Discover incredible workflow simplicity across a breadth of applications. Combining speed, high-quality
data, and the longest paired-end read lengths, the MiSeq System is ideal for targeted resequencing andsmall-genome sequencing. Optimized sample preparation kits, a streamlined next-generation sequencing(NGS) workflow, and automated, on-instrument data analysis make push-button sequencing possible.
Go from sample to data in a single day.

For Research Use Only. Not for use in diagnostic procedures.

Ease into a simple
NGS workflow.
Streamlined integration. Accelerated breakthroughs.
The MiSeq System is defined by its speed and simplicity. Its workflow is easy to use, automated,and fast. It can go from DNA to data in just over 8 hours. A compact, all-in-one platform, theMiSeq System was the first desktop sequencer to incorporate cluster generation, paired-endfluidics, sequencing by synthesis (SBS) chemistry, and complete on-instrument data analysis.
An intuitive touchscreen and convenient plug-and-play reagents with radio frequency identification(RFID) tracking make it easy to operate. The MiSeq System eliminates the need for auxiliary
hardware and computing resources, saving time and valuable bench space.

Library
preparation
Add sequencing adaptersand prepare libraries
for sequencing.

Sequencing

Data analysis

Transfer libraries to theflow cell and sequence.

Process and annotate data,reporting genomic variants
on-instrument.

For Research Use Only. Not for use in diagnostic procedures.

Final results
Interpret and reportbiological context.

For Research Use Only. Not for use in diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures.

Expand your explorations.
Far-reaching applications.
• Targeted resequencing• Small-genome sequencing• 16S metagenomics• RNA sequencing• Human leukocyte antigen (HLA) sequencing• Forensics• Preimplantation genetic screening (PGS)• Preimplantation genetic diagnosis (PGD)

Leverage integrated
bioinformatics.
Easy analysis. Fast results.
The MiSeq System is the first desktop sequencer to offer a fully integrated sequencing
ecosystem. The intuitive MiSeq Control Software guides you through the steps to start yoursequencing run. The MiSeq Reporter Software performs secondary data analysis, delivering
information on alignment and variants.
Also, you can easily access the BaseSpace® Platform computer through your MiSeq System.
Now, you can monitor your data in real time during your sequencing run, collaborate
with colleagues, and view quality performance metrics from any mobile device.
Upon run completion, the BaseSpace Platform automatically initiates the preselected
analysis and runs the report. In just a few hours, it’s at your fingertips.
Data analysis has never been easier.

Maximize performanceand productivity.
Services from product care to training topersonalized consulting.
Illumina service and support teams provide a full suite of accurate and expedientsolutions to enable researchers to find the answers they seek while delivering thepeace of mind that comes from exceptional customer service. Whether you arelooking to jump-start the implementation of your sequencing workflow, to provideadditional training for your team, or to access more strategic consulting options,
Illumina can help.
We give you full flexibility—we can customize our offerings to fit whatever yourneeds are. Our offerings include:
Product care services• Tiered instrument service plans, plus add-on services• Compliance services• On-demand services
Illumina University training• Instructor-led training• Online courses• Webinars
Illumina consulting• Proof-of-concept services for instrument and library preparation testing• Concierge services for design assistance and product optimization

For Research Use Only. Not for use in diagnostic procedures.

The gold standard in data quality.
Proven technology. Highest confidence.
Scientists around the world demand reliable data quality. More than 90% of the world’s
sequencing data is generated using Illumina SBS technology.
• Benefit from exceptional accuracy.
• Get the highest yield of error-free reads.

The performance you need.
Every Illumina sequencing system leverages our proprietary cluster generation and SBS
chemistry, the most widely adopted sequencing technology in the world. Using a single baseextension and competitive addition of nucleotides, SBS chemistry results in highly accuratesequencing that virtually eliminates homopolymer-related errors. You’ll get optimal data quality
and the utmost confidence in your results.
All Illumina sequencing systems perform fully automated paired-end sequencing, improvingalignment and genome assembly, and enabling accurate detection of structural variants, genefusions, and transcript isoforms.

> 90%of the world’s sequencing
data is generated using
Illumina SBS technology.** Data calculations on file. Illumina, Inc., 2015.

World-class solutions.
A community of support.
From sample prep, library prep, arrays, and sequencing to informatics,
Illumina next-generation solutions empower researchers and clinical researchers
across the globe to find the answers they seek.
When you join the Illumina community, you become part of a dynamic scientificmovement that includes thousands of researchers and industry thought leaders.
Throughout the year, we host user group meetings, symposia, consortia, online
forums, and other initiatives—all designed to bring the best minds together toshare ideas and advance science.
In addition to on-site training, ongoing support, and phone consults, we offer
webinars and courses at various Illumina locations. We’re here with all theresources you need to accelerate progress.

Learn more about the focused power of theMiSeq System at www.illumina.com/miseq.
A global genomics leader, Illumina provides complete next-generation sequencing workflow solutions to thebasic and translational research communities. Illumina technology is responsible for generating more than
90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking
advancements in the fields of oncology, reproductive health, genetic disease, microbiology, agriculture,and forensic science.
* Data calculations on file. Illumina, Inc., 2015.

Illumina • 1.800.809.4566 toll-free (US) • +1.858.202.4566 tel • techsupport@illumina.com • www.illumina.com
For Research Use Only. Not for use in diagnostic procedures.
© 2015 Illumina, Inc. All rights reserved. Illumina, BaseSpace, MiSeq, and the pumpkin orange color are trademarks of Illumina, Inc.
and/or its affiliate(s) in the US and/or other countries. All other names, logos, and other trademarks are the property of their respective owners.
Pub. No. 1270-2014-005 Current as of 25 September 2015