Selection Guide

Agilent PlateLoc Thermal Microplate
Sealer Consumables

Product number

Peelablealuminum

Peelablealuminum RT*

24210-001

24214-001

Pierceable
aluminum

Clearpeelable seal

Clear pierceable
thin seal

Clear permanentseal06644-001

16985-001

17318-001

24212-001

ADHESION
Peelable
Permanent
APPLICATIONS
Short Term
Short Term
(Hours to daily) –(Hours to daily) –not for long term storage not for long term storage

Compound storage,
low temp (0 °C to –80 °C)
Compound storage,
room temp (0 °C to +40 °C)**Sanger DNA sequencing(for example, ABI 3730)
APPLICATIONS (Typical)
PCR
PCR/qPCR

Optically clear for qPCRDIMENSIONS (sealing film on roll)Core diameter

76.2 mm (3") ID

76.2 mm (3") ID

76.2 mm (3") ID

76.2 mm (3") ID

76.2 mm (3") ID

76.2 mm (3") ID

Length of roll

457.2 m (1500 ft)

457.2 m (1500 ft)

500 m (1640 ft)

200 m (656 ft)

370 m (1213 ft)

500 m (1640 ft)

78 mm (3.07")

78 mm (3.07")

78 mm (3.07")

78 mm (3.07")

78 mm (3.07")

78 mm (3.07")

5,000

5,000

5,000

2,000

4,700

5,000

241.3 mm (9.5")

255 mm (10.04")

160 mm (6.3”)

203.2 mm (8")

203.2 mm (8")

208 mm (8.2")

0.0591 mm (0.002")

0.0700 mm (0.0028”)

0.025 mm (0.00098")

0.125 mm (0.005")

0.0762 mm (0.003")

0.049 mm (0.002")

115 mm (4.53")

115 mm (4.53")

115 mm (4.53")

115 mm (4.53")

115 mm (4.53")

115 mm (4.53")

Length used per plate
Plates/roll (min)Roll diameter
Thickness
Width

MICROPLATE COMPATIBILITY, AGILENT PLATELOC SEALING CONDITIONS/GUIDELINES (Heat sealing compatible microplates feature a raised rim or “chimney”surrounding each sample well and flat sealing surfaces. Conditions below are starting point recommendations for microplate sealing optimization—plate design can playa significant role in the ideal sealing conditions. Microplates of the same material may require different settings, based on thickness/geometry, etc. Optimize time andtemperature parameters for each plate type before use. Use the lowest temperature and shortest time period possible for best results. If warped microplates or plates that do
not lie flat are a challenge, or if you experience non-uniformity in seal quality, try Agilent’s PlateLoc Flexible Inserts Product Number 15818-002.)
Cyclic olefin copolymer (COC)

190 °C; 2.5 sec

185 °C; 1.5 sec

Polypropylene

170 °C; 1.2 sec

175 °C; 1.5 sec

Best Results with
Polypropylene 170 °C;
1.2 sec

110 °C; 1–1.2 sec

110 °C; 1–1.2 sec

Polystyrene

185 °C; 1.2 sec

Not Compatible

NANANA

2Permanent 175 °C;
1.2–2 sec

Product number

Peelablealuminum

Peelablealuminum RT*

Pierceable
aluminum

Clearpeelable seal

Clear pierceable
thin seal

Clear permanentseal

24210-001

24214-00106644-001

16985-001

17318-001

24212-001

Non-detected

Non-detected

Non-detected

OPTICAL PROPERTIES
Autofluorescence

NANA

NA

Optically clear (plate readercompatible)

NA

NA

NA

Transparent, visually

NANA

NA

PHYSICAL PROPERTIESWhite top,reflective bottom

Uncoloredaluminum foil

Reflective top andbottom

Opaque unsealed,transparent sealed

Opaque unsealed,transparent sealed

Clear unsealed, clearsealed

Material

Multilayer aluminum
foil/polymer laminate

Multilayer aluminum foil/polymer laminate

Aluminum foil w/
thermal sealant layer

Multilayer polymer
laminateMultilayer polymer
laminate

Multilayer polymer
laminate

O2 Barrier

Excellent

Excellent

GoodGoodGood

H2O Vapor barrier

Excellent

Excellent

GoodGoodGoodAppearance
Cross contamination/
leakage resistantEvaporation resistant

Excellent

PIERCEABILITY

Seal piercer
Metal tip/probe(Some, e.g. ABI 3730)

Handheld pipettor
plastic tips

Fair

Seal integrity range–80 °C to Plate
Softening

THERMAL PROPERTIES–80 °C to 40 °C**

–20 °C to 80 °C–20 °C to 120 °C–20 °C to 120 °C–80 °C to 110 °C* Before using the Peelable Aluminum RT heat seal, determine if your PlateLoc Thermal Microplate Sealer requires an adjustment to ensure compatibility. PlateLoc
instruments manufactured before 2011 may require adjustment by an Agilent Field Service Engineer. Please contact Agilent Automation Technical Support for more details.
** Room temperature storage of 100 % DMSO for 12 months. Short term resistance to 40 °C for a few weeks.

PlateLoc Seal Selection Guide Notes
Tested Microplates. These data were generated using thefollowing microplates or equivalent:•Polypropylene Microplate: Greiner, 650201(96-well, round well, round bottom)•Polystyrene Microplate: Greiner, 655101(96-well, round well, round bottom)

3www.agilent.com/lifesciences/automation
For Research Use Only. Not for use in diagnostic procedures.
This information is subject to change without notice.
© Agilent Technologies, Inc. 2017
Printed in the USA, November 16, 20175990-3659EN