AssayMAP Bravo Platform

Labware Reference Guide

Original Instructions

Notices
Manual Part Number

Warranty

G5496-90018 Revision C

The material contained in this document isprovided “as is,” and is subject to beingchanged, without notice, in future editions.
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January 2019

Copyright© Agilent Technologies, Inc. 2019
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WARN I N G
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Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
About this guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi
Reporting problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
1. Labware options and default settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Cartridge types and default labware settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Labware options for each application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Labware options for each utility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2. Labware photo reference. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
12 Column, Low Profile Reservoir, Natural PP. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
8 Row, Low Profile Reservoir, Natural PP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Reservoir, Seahorse 201254-100, PP, no walls, pyramid bottoms. . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Reservoir, Axygen Scientific RES-SW96-LP, 86mL pyramid bottom . . . . . . . . . . . . . . . . . . . . . . . . . 24
96 AbGene 1127, 1mL Deep Well, Square Well, Round Bottom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
96 AbGene U-Bottom Thermal Insert + AbGene 1127, Square Well, Round Bottom . . . . . . . . . . . 26
96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
96 Red PCR Insert + Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt . . . . . . . . . . . . . . . . . . . . . . . . 28
96 Corning 9017, Flat Bottom, Polystyrene, Clear. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
96 Costar 3363, PP Conical Bottom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
96 Eppendorf 30129300, PCR, Full Skirt, PolyPro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
96 Red PCR Insert + Eppendorf 30129300, PCR, Full Skirt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
96 Eppendorf 96/1000_U-bottom, Clear PolyPro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
96 Eppendorf 96/500_V-bottom, Clear PolyPro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
96 Greiner 650201, U-Bottom Standard, PolyPro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
96 Greiner 650201_U-Bottom, Clear PolyPro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
96 Greiner U-Bottom Thermal Insert + Greiner 650201_U-Bottom, Clear PolyPro . . . . . . . . . . . . . 37
96 Greiner 650207_U-Bottom, White PolyPro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
96 Greiner 651201_V-Bottom, Clear PolyPro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
96 Greiner V-Bottom Thermal Insert + Greiner 651201, V-Bottom, Clear PolyPro . . . . . . . . . . . . . 40
96 Greiner 652270, PCR, Full Skirt, PolyPro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
96 Red PCR Insert + Greiner 652270, PCR, Full Skirt, PolyPro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
96 Greiner 655101 PS Clr Rnd Well Flat Btm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
96 Greiner 675801, Half Area, Flat Bottom, UV Star . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
96 Nunc 269620, Flat Bottom, Polystyrene . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
96 Thermo Matrix 3732, V-bottom, 0.75ml Storage Tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
96 Thermo Matrix 3735, V-bottom, 500µl Storage Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
96 Thermo Matrix 3744, V-bottom, 500µl ScrewTop Storage Tubes . . . . . . . . . . . . . . . . . . . . . . . . 48
96 Waters 186005837, Clear PolyPro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
96AM 250 µL Tip Loading Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
AssayMAP Bravo Platform Labware Reference Guideiii

Contents

96AM Cartridge Rack and Receiver Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
96AM Receiver Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
96AM Cartridge Seating Station. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
96AM Tip Wash Station. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
96 V11 Manual Fill Reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
96 V11 LT 250 Tip Box 19477.002 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Bravo Plate Riser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Universal Lid PS Black . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

iv

AssayMAP Bravo Platform Labware Reference Guide

Preface
This guide provides a reference to the AssayMAP labware options that appear as
selections or labels in the Agilent Protein Sample Prep Workbench software.
This preface contains the following topics:•“About this guide” on page vi•“Reporting problems” on page viivPreface
About this guide

About this guide
Overview
This guide provides a reference to the labware that you can use on the AssayMAP
Bravo Platform.

What is new in this revision
Item

See...

Added labware options for the
Reformatting and Serial Dilution
utilities.

•“Reformatting utility v1.0 labware options” onpage 15•“Serial Dilution utility v1.0 labware options”
on page 15

Added part numbers for thermal
plate inserts.

•“96 AbGene U-Bottom Thermal Insert +AbGene 1127, Square Well, Round Bottom”
on page 26•“96 Greiner U-Bottom Thermal Insert +Greiner 650201_U-Bottom, Clear PolyPro” onpage 37•“96 Greiner V-Bottom Thermal Insert +Greiner 651201, V-Bottom, Clear PolyPro” onpage 40•“96 Thermo Matrix 3732, V-bottom, 0.75ml
Storage Tubes” on page 46•“96 Thermo Matrix 3735, V-bottom, 500µl
Storage Tubes” on page 47•“96 Thermo Matrix 3744, V-bottom, 500µl
ScrewTop Storage Tubes” on page 48•“Bravo Plate Riser” on page 57

Added labware descriptions

Software version
This guide documents the following versions or later:•

Protein Sample Prep Workbench 3.1•

VWorks Automation Control 13.1.4

Accessing the user guides
You can find the user guides for the AssayMAP applications and utilities in the
Literature Library of the Protein Sample Prep Workbench.
You can also access the user guides online in the VWorks Knowledge Base.

vi

AssayMAP Bravo Platform Labware Reference Guide

Preface
Reporting problems

Reporting problems
If you find a problem with the AssayMAP Bravo Platform, contact Agilent Automation
Solutions Technical Support. For contact information, go to https://www.agilent.com/
en/contact-us/page.
To report problems with…

Have the following information readyHardware•

Instrument serial number from the Bravo serial
number label•

Short description of the problem•

Instrument serial number from the Bravo serial
number label•

Short description of the problem•

Relevant software version number (for example,
automation control software, diagnostics software,and firmware)•

Error message text (or screen capture of the error
message dialog box)•

Relevant files, such as log files•

Cartridge type•Lot number•

Short description of the problem

SoftwareAssayMAP Cartridges

For instructions on how to resolve common error messages, see the AssayMAP Bravo
Platform Error Recovery Guide.

AssayMAP Bravo Platform Labware Reference Guidevii

1 Labware options and default settings
This section provides a reference to the labware options and default settings in theProtein Sample Prep Workbench.
The topics are:•“Cartridge types and default labware settings” on page 2•“Labware options for each application” on page 4•“Labware options for each utility” on page 121

1 Labware options and default settings
Cartridge types and default labware settings

Cartridge types and default labware settings
The following tables list the cartridge types and default labware by application and by
utility in the Protein Sample Prep Workbench. For cartridge details, see the Agilent
AssayMAP Cartridge Selection Guide.
All the applications and utilities require the 96AM Wash Station.
Note: The reservoir plates that are used for buffers can be easily washed and reused.
Table Applications: Cartridge types and default labware settings
Application

Cartridge
types

96
Eppendorf
30129300,
PCR, Full
Skirt,
PolyPro12 Column,
Low Profile
Reservoir,
Natural PP

Affinity
Purification v1.0

PA-W, PGW, SA-W

3 per run

4 per run

Fractionation v1.0

SCX, RPSIMAC Cartridge
Customization
v1.0

Fe(III)NTA

2 per run

5 per run

Immobilization
v1.0

SA-W, PAW, PG-W

3 per run

4 per run

In-Solution
Digestion: MultiPlate v1.0

None

1 per run

In-Solution
Digestion: Single
Plate v1.1*

None

1 per run

Normalization
v1.0**

None

2 per run

1 per run

On-Cartridge
Reaction v1.0*

PA-W, PGW, SA-W

3 per run

3 per run

Peptide Cleanup
v2.0

RP-S, C18

3 per run

4 per run

Phosphopeptide
Enrichment v2.0

TiO2,
Fe(III)NTA

3 per run

4 per runProtein Cleanup
v1.0

RP-W

3 per run

4 per run

96 AbGene
1127, 1 mL
Deep Well,
SquareRound
Bottom96 Greiner
650201,
U BottomStandard
PolyProclear

1 per run

6-16 per run

2 per run

96 Greiner
650207, U BottomStandard
PolyProwhite

V11 LT250
Tip Box
19477.002

8-11 perrun

1 per run

4 per run

0-4 per run

1 per run
1 per run*May also require a thermal plate insert on deck location 4.
**The Normalization application also requires two Bravo Plate Risers.

2

AssayMAP Bravo Platform Labware Reference Guide1 Labware options and default settings
Cartridge types and default labware settings
Table Utilities: Cartridge types and default labware settings
Utility

Cartridge
types

96
Eppendorf
30129300,
PCR, Full
Skirt,
PolyPro12
Column,Low
ProfileReservoir,
NaturalPP

96
Greiner
650201,
U BottomStandard
PolyProclear

96
Greiner
650207,
U BottomStandard
PolyProwhite

96
Greiner
655101PS ClrRnd WellFlat Btm

96
Greiner
675801,
Half Area,
Flat
Bottom,UV StarReservoir,
Seahorse
201254100, PP,
no walls,
pyramidbottoms

V11
LT250
Tip Box
19477.
002

Cartridge
Transfer
v1.0

All types

PipetteTip
Transfer
v1.0

None

Reagent
Aliquot
v1.0

None

2 per run

1 per
run

Reagent
Transfer
v2.0

None

2 per run

1 per
run,
optional

Reformatting v1.0

None

2 per run

1 per
run

SerialDilution
v1.0

None

1 per runSingleLiquidAddition
v1.0

None

Syringe
Test v1.0

Resin
Free

Syringe
Wash v2.0

None

SystemStartup/Shutdown
v2.1

None

1 per
run

1 per run

1 per run

1 per
run

5–8 perrun

2 per run

2 per run
1–6 perrun

0–1 perrun

AssayMAP Bravo Platform Labware Reference Guide3

1 Labware options and default settings
Labware options for each application

Labware options for each application
The topics in this section are:

IMPORTANT•“AssayMAP Cartridges and pipette tips” on page 4•“Affinity Purification v1.0 labware options” on page 4•“Fractionation v1.0 labware options” on page 5•“IMAC Cartridge Customization v1.0 labware options” on page 5•“Immobilization v1.0 labware options” on page 6•“In-Solution Digestion: Multi-Plate v1.0 labware options” on page 6•“In-Solution Digestion: Single Plate v1.1 labware options” on page 7•“Normalization v1.0 labware options” on page 8•“On-Cartridge Reaction v1.0 labware options” on page 9•“Peptide Cleanup v2.0 labware options” on page 10•“Phosphopeptide Enrichment v2.0 labware options” on page 10•“Protein Cleanup v1.0 labware options” on page 11

See the “Labware photo reference” on page 19 for dimensionally equivalent alternatives
to the part numbers listed in the following tables.

AssayMAP Cartridges and pipette tips
Most AssayMAP applications require cartridges preloaded in the 96AM Cartridge & Tip
Seating Station (Agilent G5409-20025) at deck location 2. For cartridge details, see theAgilent AssayMAP Cartridge Selection Guide.
The In-Solution Digestion and Normalization applications have the option of using
250-µL pipette tips (Agilent 19477-002) in the 96AM Cartridge & Tip Seating Station atdeck location 2.

Affinity Purification v1.0 labware options

4

LabwareManufacturer part number

Deck location
options

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

3, 5, 6, 7, 8

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

3, 5, 6, 7, 8

96 ABgene 1127, 1mL Deep Well, Square Well, Round
BottomABgene AB-1127

3, 4, 5, 6, 7, 8

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

4, 7, 996 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

4, 7, 996 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

3, 4, 5, 6, 7, 8, 9

96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

3, 4, 5, 6, 7, 8, 9

96 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

3, 4, 5, 6, 7, 8, 9

AssayMAP Bravo Platform Labware Reference Guide1 Labware options and default settings
Labware options for each application
LabwareManufacturer part number

Deck location
options

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

3, 4, 5, 6, 7, 8, 9

96 Greiner 675801, Half Area, Flat Bottom, UV Star

Greiner 675801

4, 7, 996 V11 Manual Fill Reservoir

Agilent G5498B#049

3, 5, 6, 8

Fractionation v1.0 labware options
LabwareManufacturer part number

Deck location options

96 ABgene 1127, 1 mL Deep Well, Square Well,
Round Bottom

ABgene AB-1127

396 Greiner 650201, U-Bottom Standard PolyPro

Greiner 650201

4, 5, 6, 7, 9

IMAC Cartridge Customization v1.0 labware options
LabwareManufacturer part number

Deck location
options

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

3, 4, 5, 6, 7, 8, 9

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

3, 4, 5, 6, 7, 8, 9

96 ABgene 1127, 1mL Deep Well, Square Well, Round
BottomABgene AB-1127

3, 4, 5, 6, 7, 8, 9

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

7, 9

96 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

7, 9

96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt

Bio-Rad HSP-9611

7, 9

96 Greiner 650201 U-Bottom, Clear PolyPro

Greiner 650201

3, 4, 5, 6, 7, 8, 9

96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

3, 4, 5, 6, 7, 8, 9

96 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

3, 4, 5, 6, 7, 8, 9

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

3, 4, 5, 6, 7, 8, 9

96 Greiner 675801, Half Area, Flat-Bottom, UV Star

Greiner 675801

7, 9

96 V11 Manual Fill Reservoir

Agilent G5498B#049

4, 5, 6, 8, 9Reservoir, Axygen Scientific RES-SW96-LP, 86mL,
pyramid bottom

Axygen Scientific
RES-SW96-LP

4, 5, 6, 8, 9Reservoir, Seahorse 201254-100, PP, no walls, pyramidbottoms

Agilent 201254-100

4, 5, 6, 8, 9

AssayMAP Bravo Platform Labware Reference Guide5

1 Labware options and default settings
Labware options for each application

Immobilization v1.0 labware options
LabwareManufacturer partnumberDeck location
options

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

3, 5, 6, 7, 8

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

3, 5, 6, 7, 8

96 ABgene 1127, 1mL Deep Well, Square Well, Round
BottomABgene AB-1127

3, 4, 5, 6, 7, 8

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

4, 7, 996 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

4, 7, 996 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

3, 4, 5, 6, 7, 8, 9

96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

3, 4, 5, 6, 7, 8, 9

96 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

3, 4, 5, 6, 7, 8, 9

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

3, 4, 5, 6, 7, 8, 9

96 Greiner 675801, Half Area, Flat-Bottom, UV Star

Greiner 675801

4, 7, 996 V11 Manual Fill Reservoir

Agilent G5498B#049

3, 5, 6, 8

In-Solution Digestion: Multi-Plate v1.0 labware options
LabwareManufacturer partnumberDeck location
options

Reagent Setup•

96 Red PCR Insert + 96 Eppendorf 30129300, PCR,
Full Skirt

Agilent insert (provided)and Eppendorf 30129300

4•96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

5, 7

•

250-µL pipette tips

Agilent 19477-002

6•96 ABgene 1127, Deep Well, Square Well, Round
BottomABgene AB-1127

8

In-Solution Digestion

6•

96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

2, 5, 6, 7, 8, 9•

96 ABgene 1127, Deep Well, Square Well, Round
BottomABgene AB-1127

3•

96 Red PCR Insert + 96 Eppendorf 30129300, PCR,
Full Skirt

Agilent insert (provided)and Eppendorf 30129300

4

AssayMAP Bravo Platform Labware Reference Guide1 Labware options and default settings
Labware options for each application

In-Solution Digestion: Single Plate v1.1 labware options
LabwareManufacturer part number

Deck location
options

Lid, Universal*

Agilent 200858-100

4, 5, 6, 8, 9

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

4, 5, 6, 8, 9

96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt

Bio-Rad HSP-9611

4, 5, 6, 8, 9

96 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

4, 5, 6, 8, 9

96 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

4, 5, 6, 8, 9

96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

4, 5, 6, 8, 9

96 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

4, 5, 6, 8, 9

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

4, 5, 6, 8, 9

96 ABgene 1127, 1mL Deep Well, Square Well, Round
BottomABgene AB-1127

4, 5, 6, 8, 9

96 Eppendorf 96/500_V-bottom, Clear PolyPro

Eppendorf 96/500

4, 5, 6, 8, 9

96 Eppendorf 96/1000_U-bottom, Clear PolyPro

Eppendorf 96/1000

4, 5, 6, 8, 9

96 Waters 186005837, Clear PolyPro

Waters 186005837

4, 5, 6, 8, 9

12 Seahorse, Low Profile Reservoir, Natural PP Lidded

Agilent 201280-100

5, 6, 8, 9

8 Seahorse, Low Profile Reservoir, Natural PP Lidded

Agilent 201282-100

5, 6, 8, 9Reservoir, Seahorse 201254-100, PP, 1 Well, pyramidbottomAgilent 201254-100

5, 6, 8, 9Reservoir, Axygen Scientific RES-SW96-LP, 86mL
pyramid bottom

Axygen Scientific
RES-SW96-LP

5, 6, 8, 9

96 V11 Manual Fill Reservoir

Agilent G5498B#049

5, 6, 8, 9

96 Red PCR Insert + Eppendorf 30129300, PCR, Full
Skirt

Agilent insert (provided)and Eppendorf 30129300

496 Red PCR Insert + Bio-Rad PCR, Hard-Shell, LowProfile, Full Skirt

Agilent insert (provided)and Bio-Rad HSP-9611

4

96 Red PCR Insert + Greiner 652270, PCR, Full Skirt,
PolyProAgilent insert (provided)and Greiner 652270

496 Greiner U-Bottom Thermal Insert + Greiner
650201_U-Bottom, Clear PolyPro

Agilent G5498B#126 andGreiner 650201

496 Greiner V-Bottom Thermal Insert + Greiner
651201_V-Bottom, Clear PolyPro

Agilent G5498B#126 andGreiner 651201

4

AssayMAP Bravo Platform Labware Reference Guide7

1 Labware options and default settings
Labware options for each application
LabwareManufacturer part number

Deck location
options

96 AbGene U-Bottom Thermal Insert + AbGene 1127,
Square Well, Round Bottom

Agilent G5498B#127 andABgene AB-1127

4*Lidded plates are allowed at these deck locations, not the lid by itself. If you plan to use a lidded plate, ensurethat you use only the approved lid type.

Normalization v1.0 labware options

8

LabwareManufacturer part number

Deck location
options

96 ABgene 1127, 1mL Deep Well, Square Well, Round
BottomABgene AB-1127

2, 6

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

2, 6

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

2, 6

96 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

2, 696 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

2, 696 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

2, 6

96 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

2, 696 Greiner 675801, Half Area, Flat-Bottom, 
UV StarGreiner 675801

2, 6

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

48 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

4

96 V11 Manual Fill Reservoir

Agilent G5498B#049

4

Reservoir, Axygen Scientific RES-SW96-LP, 86mL
pyramid bottom

Axygen Scientific
RES-SW96-LP

4

AssayMAP Bravo Platform Labware Reference Guide1 Labware options and default settings
Labware options for each application

On-Cartridge Reaction v1.0 labware options
LabwareManufacturer part number

Deck location
options

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

3—9

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

3—9

Reservoir, Seahorse 201254-100, PP, no walls, pyramidbottoms

Agilent 201254-100

3—9

Reservoir, Axygen Scientific RES-SW96-LP, 86mL
pyramid bottom

Axygen Scientific RESSW96-LP

3—9

96 ABgene 1127, 1mL Deep Well, Square Well, Round
BottomABgene AB-1127

3—9

96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt

Bio-Rad HSP-9611

3—9

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

3—9

96 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

3—9

96 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

3—9

96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

3—9

96 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

3—9

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

3—9

96 Greiner 675801, Half Area, Flat-Bottom, UV Star

Greiner 675801

3—9

96 V11 Manual Fill Reservoir

Agilent G5498B#049

3—9

96 Red PCR Insert + Eppendorf 30129300, PCR, Full Skirt

Agilent insert (provided)and Eppendorf 30129300

496 Red PCR Insert + Bio-Rad PCR, Hard-Shell,
Low-Profile, Full Skirt

Agilent insert (provided)and Bio-Rad HSP-9611

4

96 Red PCR Insert + Greiner 652270, PCR, Full Skirt,
PolyProAgilent insert (provided)and Greiner 652270

496 Greiner U-Bottom Thermal Insert + Greiner 650201_UBottom, Clear PolyPro

Agilent G5498B#126 andGreiner 650201

496 Greiner V-Bottom Thermal Insert + Greiner 651201 VBottom, Clear PolyPro

Agilent G5498B#126 andGreiner 651201

496 AbGene U-Bottom Thermal Insert + AbGene 1127,
Square Well, Round Bottom

Agilent G5498B#127 andABgene AB-1127

4

AssayMAP Bravo Platform Labware Reference Guide9

1 Labware options and default settings
Labware options for each application

Peptide Cleanup v2.0 labware options
LabwareManufacturer partnumberDeck location
options

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

3, 5, 6, 7, 8

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

3, 5, 6, 7, 8

96 ABgene 1127, 1mL Deep Well, Square Well, Round
BottomABgene AB-1127

3, 4, 5, 6, 7, 8

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

4, 7, 996 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

4, 7, 996 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

3, 4, 5, 6, 7, 8, 9

96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

3, 4, 5, 6, 7, 8, 9

96 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

3, 4, 5, 6, 7, 8, 9

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

3, 4, 5, 6, 7, 8, 9

96 Greiner 675801, Half Area, Flat-Bottom, UV Star

Greiner 675801

4, 7, 996 V11 Manual Fill Reservoir

Agilent G5498B#049

5, 6, 8

Phosphopeptide Enrichment v2.0 labware options
LabwareManufacturer part number

Deck location
options

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

3, 5, 6, 7, 8

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

3, 5, 6, 7, 8

96 ABgene 1127, 1mL Deep Well, Square Well, Round
BottomABgene AB-1127

3, 4, 5, 6, 7, 8

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

4, 7, 996 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

4, 7, 996 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt

Bio-Rad HSP-9611

4, 7, 996 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

3, 4, 5, 6, 7, 8, 9

96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

3, 4, 5, 6, 7, 8, 9

96 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

3, 4, 5, 6, 7, 8, 9

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

3, 4, 5, 6, 7, 8, 9

96 Greiner 675801, Half Area, Flat-Bottom, 
UV StarGreiner 675801

4, 7, 996 V11 Manual Fill Reservoir

Agilent G5498B#049

5, 6, 8

10

AssayMAP Bravo Platform Labware Reference Guide1 Labware options and default settings
Labware options for each application
LabwareManufacturer part number

Deck location
options

Reservoir, Axygen Scientific RES-SW96-LP, 86mL
pyramid bottom

Axygen Scientific
RES-SW96-LP

5, 6, 8Reservoir, Seahorse 201254-100, PP, no walls, pyramidbottoms

Agilent 201254-100

5, 6, 8LabwareManufacturer partnumberDeck location options

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

3, 5, 6, 7, 8

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

3, 5, 6, 7, 8

96 ABgene 1127, 1mL Deep Well, Square Well, Round
BottomABgene AB-1127

3, 4, 5, 6, 7, 8

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

4, 7, 996 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

4, 7, 996 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

3, 4, 5, 6, 7, 8, 9

96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

3, 4, 5, 6, 7, 8, 9

96 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

3, 4, 5, 6, 7, 8, 9

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

3, 4, 5, 6, 7, 8, 9

96 Greiner 675801, Half Area, Flat-Bottom, 
UV StarGreiner 675801

4, 7, 996 V11 Manual Fill Reservoir

Agilent G5498B#049

5, 6, 8

Protein Cleanup v1.0 labware options

AssayMAP Bravo Platform Labware Reference Guide11

1 Labware options and default settings
Labware options for each utility

Labware options for each utility
This section includes the following tables:•“Cartridge Transfer utility v1.0 required labware” on page 12•“Pipette Tip Transfer utility v1.0 required labware” on page 12•“Reagent Aliquot utility v1.0 labware options” on page 13•“Reagent Transfer utility v2.0 labware options” on page 14•“Reformatting utility v1.0 labware options” on page 15•“Serial Dilution utility v1.0 labware options” on page 15•“Single Liquid Addition utility v1.0 labware options” on page 16•“Syringe Test utility v1.0 labware options” on page 17•“Syringe Wash utility v2.0 labware options” on page 17•“System Startup/Shutdown utility v2.1 labware options” on page 18

IMPORTANT See the “Labware photo reference” on page 19 for dimensionallyequivalent alternatives to the part numbers listed in the following tables.

Cartridge Transfer utility v1.0 required labware
LabwareManufacturer part number

Deck location

96AM Cartridge & Tip Seating Station

Agilent G5409-20025

2

96AM Cartridge Rack and Receiver Plate withcartridges (all types)

See Agilent AssayMAP
Cartridge Selection Guide

6

Pipette Tip Transfer utility v1.0 required labware
LabwareManufacturer part number

Deck location

96AM Cartridge & Tip Seating Station

Agilent G5409-20025

2

96 V11 LT 250 Tip Box (250-µL pipette tips)

Agilent 19477-002

6

12

AssayMAP Bravo Platform Labware Reference Guide1 Labware options and default settings
Labware options for each utility

Reagent Aliquot utility v1.0 labware options
LabwareManufacturer part number

Deck location options

96AM Tip Loading Station (required)

Agilent G5409-20025

2

96 V11 LT 250 Tip Box (250-µL pipette tips)(required)

Agilent 19477-002

6

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

4, 7

96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt

Bio-Rad HSP-9611

4, 7

96 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

4, 7

96 ABgene 1127, 1mL Deep Well, Square Well,
Round Bottom

ABgene AB-1127

4, 7

96 Eppendorf 96/500 V-bottom, Clear, PolyPro

Eppendorf 96/500

4, 7

96 Eppendorf 96/1000 U-bottom, Clear, PolyPro

Eppendorf 96/1000

4, 7

96 Waters 186005837, Clear PolyPro

Waters 186005837

4, 7

96 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

4, 796 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

4, 796 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

4, 7

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

4, 7

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

7

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

7

Reservoir, Seahorse 201254-100, PP, no walls,
pyramid bottoms

Agilent 201254-100

7

Reservoir, Axygen Scientific RES-SW96-LP, 86mL
pyramid bottom

Axygen Scientific RES-SW96LP7

96 V11 Manual Fill Reservoir

Agilent G5498B#049

7

AssayMAP Bravo Platform Labware Reference Guide13

1 Labware options and default settings
Labware options for each utility

Reagent Transfer utility v2.0 labware options
LabwareManufacturer part number

Deck location
options

96AM Cartridge & Tip Seating Station

Agilent G5409-20025

2

96 V11 LT 250 Tip Box 19477-002

Agilent 19477-002

6

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

7, 8

96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt

Bio-Rad HSP-9611

7, 8

96 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

7, 8

96 ABgene 1127, 1mL Deep Well, Square Well, Round
BottomABgene AB-1127

7, 8

96 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

7, 8

96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

7, 896 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

7, 8

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

7, 8

96 Greiner 675801, Half Area, Flat-Bottom, UV Star

Greiner 675801

7, 8

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

7, 8

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

7, 8

Reservoir, Seahorse 201254-100, PP, no walls, pyramidbottomAgilent 201254-100

7, 8

Reservoir, Axygen Scientific RES-SW96-LP, 86mL pyramidbottomAxygen Scientific
RES-SW96-LP

7, 8

96 V11 Manual Fill Reservoir

Agilent G5498B#049

7, 896AM Receiver Plate

Included with Agilent
cartridge rack

7, 8

96 Eppendorf 96/500 V-bottom, Clear, PolyPro*

Eppendorf 96/500

7, 8

96 Eppendorf 96/1000 U-bottom, Clear, PolyPro*

Eppendorf 96/1000

7, 8

96 Waters 186005837, Clear PolyPro*

Waters 186005837

7, 8*Pipette tips are required for reagent transfers using these deep-well plate options.

14

AssayMAP Bravo Platform Labware Reference Guide1 Labware options and default settings
Labware options for each utility

Reformatting utility v1.0 labware options
LabwareManufacturer part number

Deck location
options

96AM Tip Loading Station (required)

Agilent G5409-20025

5

96 V11 LT 250 Tip Box (250-µL pipette tips) (required)

Agilent 19477-002

396 ABgene 1127, 1mL Deep Well, Square Well, Round Bottom

ABgene AB-1127

2, 6

96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt

Bio-Rad HSP-9611

2, 6

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

2, 6

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

2, 6

96 Eppendorf 96/500_V-bottom, Clear PolyPro

Eppendorf 96/500

2, 6

96 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

2, 696 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

2, 696 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

2, 6

96 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

2, 696 Greiner 675801, Half Area, Flat-Bottom, UV Star

Greiner 675801

2, 6

96 Thermo Matrix 3732, V-bottom, 0.75ml Storage Tubes

Thermo Fisher Scientific
3732

2, 6

96 Thermo Matrix 3735, V-bottom, 500µl Storage Tubes

Thermo Fisher Scientific
3735

2, 6

96 Thermo Matrix 3744, V-bottom, 500µl ScrewTop Storage
Tubes

Thermo Fisher Scientific
3744

2, 6

96 Waters 186005837, Clear PolyPro

Waters 186005837

2, 6

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

2, 6

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

2, 6

Serial Dilution utility v1.0 labware options
LabwareManufacturer part number

Deck location

96AM Tip Loading Station (required)

Agilent G5409-20025

5

96 V11 LT 250 Tip Box (250-µL pipette tips) (required)

Agilent 19477-002

3

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

212 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

2

96 V11 Manual Fill Reservoir

Agilent G5498B#049

2

AssayMAP Bravo Platform Labware Reference Guide15

1 Labware options and default settings
Labware options for each utility
LabwareManufacturer part number

Deck location

Reservoir, Seahorse 201254-100, PP, no walls, pyramidbottomAgilent 201254-100

296 AbGene 1127, 1mL Deep Well, Square Well, Round
BottomABgene AB-1127

2, 6

96 Costar 3363, PP Conical Bottom

Corning Costar 3363

2, 6

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

Eppendorf 30129300

2, 6

96 Eppendorf 96/500_V-bottom, Clear PolyPro

Eppendorf 96/500

2, 6

96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt

Bio-Rad HSP-9611

2, 6

96 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

2, 696 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

2, 696 Greiner 651201_V-Bottom, Clear PolyPro

Greiner 651201

2, 6

96 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

2, 696 Greiner 675801, Half Area, Flat-Bottom, UV Star

Greiner 675801

6

96 Thermo Matrix 3732, V-bottom, 0.75ml Storage
Tubes

Thermo Fisher Scientific 3732

2, 6

96 Thermo Matrix 3735, V-bottom, 500µl Storage
Tubes

Thermo Fisher Scientific 3735

2, 6

96 Thermo Matrix 3744, V-bottom, 500µl ScrewTop
Storage Tubes

Thermo Fisher Scientific 3744

2, 6

96 Waters 186005837, Clear PolyPro

Waters 186005837

2, 6Single Liquid Addition utility v1.0 labware options
LabwareManufacturer part number

Deck location options

96 Greiner 650207_U-Bottom, White PolyPro

Greiner 650207

2, 5, 6, 8, 9

96 Eppendorf 30129300, PCR, Full Skirt,
PolyProEppendorf 30129300

496 Greiner 650201_U-Bottom, Clear PolyPro

Greiner 650201

496 Greiner 652270, PCR, Full Skirt, PolyPro

Greiner 652270

496 Costar 3363, PP Conical Bottom

Corning Costar 3363

4

96 ABgene 1127, 1mL Deep Well, Square Well,
Round Bottom

ABgene AB-1127

412 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

48 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

416

AssayMAP Bravo Platform Labware Reference Guide1 Labware options and default settings
Labware options for each utility
96 V11 Manual Fill Reservoir

Agilent G5498B#049

4

Syringe Test utility v1.0 labware options
LabwareManufacturer part number

Deck location options

96AM Cartridge Seating Station (required)

Agilent G5409-20025

2

Reservoir, Seahorse 201254-100, PP, nowalls, pyramid bottoms

Agilent 201254-100

4, 7

Reservoir, Axygen Scientific RES-SW96-LP,
86mL pyramid bottom

Axygen Scientific RES-SW96LP

4, 7

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

4, 7

96 V11 Manual Fill Reservoir

Agilent G5498B#049

4, 7

Resin Free cartridges in 96AM Cartridge Rack
and Receiver Plate (required)

Agilent G5496-60008

696 Greiner 655101 PS Clr Rnd Well Flat Btm

Greiner 655101

8, 9

96 Nunc 269620, Flat Bottom, Polystyrene

Nunc 269620

8, 9

96 Corning 9017, Flat bottom, polystyrene,
clear

Corning 9017

8, 9

Syringe Wash utility v2.0 labware options
LabwareManufacturer part number

Deck location options

96AM Cartridge & Tip Seating Station

Agilent G5409-20025

2

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

3–9

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

3–9

96 Greiner 650201 U-Bottom, Standard,
PolyProGreiner 650201

3–9

96 ABgene 1127, 1mL Deep Well, Square Well,
Round Bottom

ABgene AB-1127

3–9

Reservoir, Seahorse 201254-100, PP, nowalls, pyramid bottom

Agilent 201254-100

3–9

Reservoir, Axygen Scientific RES-SW96-LP,
86mL pyramid bottom

Axygen Scientific
RES-SW96-LP

3–9

96 V11 Manual Fill Reservoir

Agilent G5498B#049

3–9

AssayMAP Bravo Platform Labware Reference Guide17

1 Labware options and default settings
Labware options for each utility

System Startup/Shutdown utility v2.1 labware options
LabwareManufacturer part number

Deck location options

96AM Cartridge & Tip Seating Station

Agilent G5409-20025

2

12 Column, Low Profile Reservoir, Natural PP

Agilent 201280-100

7

8 Row, Low Profile Reservoir, Natural PP

Agilent 201282-100

7

Reservoir, Seahorse 201254-100, PP, nowalls, pyramid bottoms

Agilent 201254-100

7

Reservoir, Axygen Scientific RES-SW96-LP,
86mL pyramid bottom

Axygen Scientific RES-SW96LP

7

96 Greiner 650201, U-Bottom, Standard,
PolyProGreiner 650201

796 ABgene 1127, 1mL Deep Well, Square Well,
Round Bottom

ABgene AB-1127

7

96 V11 Manual Fill Reservoir

Agilent G5498B#049

7

18

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo referenceThis section provides a photograph and description of each labware used in theProtein Sample Prep Workbench.
•“12 Column, Low Profile Reservoir, Natural PP” on page 21•“8 Row, Low Profile Reservoir, Natural PP” on page 22•“Reservoir, Seahorse 201254-100, PP, no walls, pyramid bottoms” on page 23•“Reservoir, Axygen Scientific RES-SW96-LP, 86mL pyramid bottom” on page 24•“96 AbGene 1127, 1mL Deep Well, Square Well, Round Bottom” on page 25•“96 AbGene U-Bottom Thermal Insert + AbGene 1127, Square Well, Round
Bottom” on page 26•“96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt” on page 27•“96 Red PCR Insert + Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt” onpage 28•“96 Corning 9017, Flat Bottom, Polystyrene, Clear” on page 29•“96 Costar 3363, PP Conical Bottom” on page 30•“96 Eppendorf 30129300, PCR, Full Skirt, PolyPro” on page 31•“96 Red PCR Insert + Eppendorf 30129300, PCR, Full Skirt” on page 32•“96 Eppendorf 96/1000_U-bottom, Clear PolyPro” on page 33•“96 Eppendorf 96/500_V-bottom, Clear PolyPro” on page 34•“96 Greiner 650201, U-Bottom Standard, PolyPro” on page 35•“96 Greiner 650201_U-Bottom, Clear PolyPro” on page 36•“96 Greiner U-Bottom Thermal Insert + Greiner 650201_U-Bottom, Clear
PolyPro” on page 37•“96 Greiner 650207_U-Bottom, White PolyPro” on page 38•“96 Greiner 651201_V-Bottom, Clear PolyPro” on page 39•“96 Greiner V-Bottom Thermal Insert + Greiner 651201, V-Bottom, Clear
PolyPro” on page 40•“96 Greiner 652270, PCR, Full Skirt, PolyPro” on page 41•“96 Red PCR Insert + Greiner 652270, PCR, Full Skirt, PolyPro” on page 42•“96 Greiner 655101 PS Clr Rnd Well Flat Btm” on page 43•“96 Greiner 675801, Half Area, Flat Bottom, UV Star” on page 44•“96 Nunc 269620, Flat Bottom, Polystyrene” on page 45•“96 Thermo Matrix 3732, V-bottom, 0.75ml Storage Tubes” on page 46•“96 Thermo Matrix 3735, V-bottom, 500µl Storage Tubes” on page 47•“96 Thermo Matrix 3744, V-bottom, 500µl ScrewTop Storage Tubes” on page 48•“96 Waters 186005837, Clear PolyPro” on page 49

19

2 Labware photo reference

20•“96AM 250 µL Tip Loading Station” on page 50•“96AM Cartridge Rack and Receiver Plate” on page 51•“96AM Receiver Plate” on page 52•“96AM Cartridge Seating Station” on page 53•“96AM Tip Wash Station” on page 54•“96 V11 Manual Fill Reservoir” on page 55•“96 V11 LT 250 Tip Box 19477.002” on page 56•“Bravo Plate Riser” on page 57•“Universal Lid PS Black” on page 58

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
12 Column, Low Profile Reservoir, Natural PP

12 Column, Low Profile Reservoir, Natural PP
Manufacturer: Agilent Technologies
Part number: 201280-100
Name of labware type in the software: 
12 Column, Low Profile Reservoir, Natural PP or 12
Seahorse, Low Profile, Reservoir, Natural PP

General information
One of the most common reagent reservoir options used in the Protein Sample Prep
Workbench. The column-wise partitions enable partial-plate runs with the same fullcolumn convention that is used to define the number of full columns of cartridges or
pipette tips in most applications and utilities.

Volume recommendations
Number of wells = 12
Maximum volume per well = 7 mL
Recommended overage per well = 3 mL

Additional notes
A dimensionally equivalent alternative is the Axygen (Corning) RES-MW12-LP.

Resources
For information about...

See...

Ordering

https://www.chem.agilent.com/store/

AssayMAP Bravo Platform Labware Reference Guide21

2 Labware photo reference
8 Row, Low Profile Reservoir, Natural PP

8 Row, Low Profile Reservoir, Natural PPManufacturer: Agilent Technologies
Part number: 201282-100
Name of labware type in the software: 
8 Row, Low Profile Reservoir, Natural PP or
8 Seahorse, Low Profile Reservoir, Natural PP

General information
This is a common reagent reservoir option in the Protein Sample Prep Workbench. The
row-wise partitions enable partial-plate runs based on full rows of 12 samples. This
reservoir also makes it easy to isolate reagents if experimentation involves testing
reagents with different compositions.

Volume recommendations
Number of wells = 8
Maximum volume per well = 10.6 mL
Recommended overage per well = 4.5 mL

Additional notes
A dimensionally equivalent alternative is the Axygen (Corning) RES-MW8-LP.

Resources

22

For information about...

See...

Ordering

https://www.chem.agilent.com/store/

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
Reservoir, Seahorse 201254-100, PP, no walls, pyramid bottoms

Reservoir, Seahorse 201254-100, PP, no walls, pyramid bottoms
Manufacturer: Agilent Technologies
Part number: 201254-100
Name of labware type in the software: Reservoir, Seahorse 201254-100, PP, no walls,
pyramid bottoms

General information
This common reservoir is very useful for AssayMAP operations. It enables easy fillingwhen running a full plate of 96 samples.

Volume recommendations
Number of wells = 1
Maximum volume per well = 86 mL
Recommended overage per well = 20 mL

Additional notes
A dimensionally equivalent alternative is the Axygen (Corning) RES-SW96-LP.

Resources
For information about...

See...

Ordering

https://www.chem.agilent.com/store/

AssayMAP Bravo Platform Labware Reference Guide23

2 Labware photo reference
Reservoir, Axygen Scientific RES-SW96-LP, 86mL pyramid bottom

Reservoir, Axygen Scientific RES-SW96-LP, 86mL pyramid bottom
Manufacturer: Axygen ScientificPart number: RES-SW96-LP
Name of labware type in the software: Reservoir, Axygen Scientific RES-SW96-LP, 86mL
pyramid bottom

General information
This common reservoir is very useful for AssayMAP operations. It enables easy fillingwhen running a full plate of 96 samples.

Volume recommendations
Number of wells = 1
Maximum volume per well = 86 mL
Recommended overage per well = 20 mL

Additional notes
A dimensionally equivalent alternative is the Reservoir, Seahorse 201254-100, PP, nowalls, pyramid bottoms.

Resources24

For information about...

See...

Product page

http://catalog2.corning.com/LifeSciences/en-US/
Shopping/
ProductDetails.aspx?categoryname=&productid=RESSW96-LP(Lifesciences)&vid

Ordering information

http://www.corning.com/worldwide/en/products/lifesciences/how-to-buy.html

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 AbGene 1127, 1mL Deep Well, Square Well, Round Bottom

96 AbGene 1127, 1mL Deep Well, Square Well, Round Bottom
Manufacturer: Fisher ScientificPart number: AB-1127
Name of labware type in the software:
96 AbGene 1127, 1mL Deep Well, Square Well, Round
BottomGeneral information
The AbGene 1127 is a very important labware type for the AssayMAP Bravo Platform
because it is the only known type of deep-well plate that can be used with the
AssayMAP head for bare probe operations. The plate has a low enough profile toenable the probes of the head to reach the bottom of the plate without making contact
with the cartridges or the head stripper plate.

Volume recommendations
Number of wells = 96
Maximum volume per well = 1050 µL
Recommended overage per well = 30 µL

Resources
For information about...

See...

Product pagehttps://www.thermofisher.com/order/catalog/product/
AB1127

Technical drawing

https://tools.thermofisher.com/content/sfs/manuals/
MAN0014419_ABgene_Storage_Plate_96well_1_2mL_QR.
pdf

AssayMAP Bravo Platform Labware Reference Guide25

2 Labware photo reference
96 AbGene U-Bottom Thermal Insert + AbGene 1127, Square Well, Round Bottom

96 AbGene U-Bottom Thermal Insert + AbGene 1127, Square
Well, Round Bottom
Manufacturer and part number:•

AbGene plate: Fisher Scientific AB-1127•

96 Abgene Deep Well Insert: Agilent G5498B#127

Name of labware type in the software:
96 AbGene U-Bottom Thermal Insert + AbGene 1127,
Square Well, Round Bottom

General information
In applications that require heating of the AbGene 1127 plate, place the AbGene 1127
plate atop the 96 AbGene U-Bottom Thermal Insert to allow efficient heat transfer fromthe Peltier Thermal Station to the AbGene 1127 plate. The combination of the AbGene
1127 plate stacked on the thermal insert results in a different plate height than the
AbGene 1127 plate alone. The combination is designated in the labware selection as
96 AbGene U-Bottom Thermal Insert + AbGene 1127, Square Well, Round Bottom

Volume recommendations
Number of wells = 96
Maximum volume per well = 1050 µL
Recommended overage per well = 30 µL

Resources26

For information
about...

See...

AbGene 1127 platehttps://www.thermofisher.com/order/catalog/product/
AB1127

Thermal Insert

https://www.agilent.com/en/products/automationsolutions/protein-sample-preparation/assaymap-bravoplatform
AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt

96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt
Manufacturer: Bio-Rad
Part number: HSP-9611 (See Additional notes.)Name of labware type in the software: 
96 Bio-Rad PCR, Hard-Shell, Low-Profile, Full
Skirt

General information
This is one of three types of PCR plates that can be used with the Protein Sample Prep
Workbench. Any of the many color varieties of this plate type can be used. The
elongated conical geometry of PCR style plates, results in the smallest overage volume
requirements.

Volume recommendations
Number of wells = 96
Maximum volume per well = 210 µL
Recommended overage per well = 10 µL

Additional notes
Dimensionally equivalent alternatives are the Bio-Rad Hard-Shell, Low-Profile plates,
including HSP-9601, 9901, 9621, 9631, 9641, 9661, 9655, 9955, 9665, 9635, 9645, and
9666.

Resources
For information about...

See...

Product page andtechnical drawing

http://www.bio-rad.com/en-us/product/hard-shell-96well-pcr-plates/hard-shell-96-well-skirted-pcr-plates-lowprofile

AssayMAP Bravo Platform Labware Reference Guide27

2 Labware photo reference
96 Red PCR Insert + Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt

96 Red PCR Insert + Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt
Manufacturer and part number:•

PCR plate: Bio-Rad HSP-9611•

Red PCR Plate Insert: Agilent G5498B#013

Name of labware type in the software:
96 Red PCR Insert + Bio-Rad PCR, Hard-Shell, Low-Profile, Full
Skirt

General information
In applications that require heating the BioRad PCR plate, place the BioRad PCR plate
atop the Red PCR Insert to allow efficient heat transfer from the Peltier Thermal Station
to the BioRad PCR plate. The combination of the BioRad PCR plate stacked on thethermal insert results in a different plate height than the BioRad PCR plate alone.
Therefore, the combination is designated in the labware selection as
96 Red PCR Insert + Bio-Rad PCR, Hard-Shell, Low-Profile, Full Skirt

Volume recommendations
Number of wells = 96
Maximum volume per well = 210 µL
Recommended overage per well = 10 µL

Resources28

For information
about...

See...

Bio-Rad PCR
plate

http://www.bio-rad.com/en-us/product/hard-shell-96-well-pcrplates/hard-shell-96-well-skirted-pcr-plates-low-profile

Red PCR Plate
Inserthttps://www.agilent.com/en/products/automation-solutions/protein-sample-preparation/assaymap-bravo-platform

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 Corning 9017, Flat Bottom, Polystyrene, Clear

96 Corning 9017, Flat Bottom, Polystyrene, Clear
Manufacturer: Corning
Part number: 9017
Name of labware type in the software: 96 Corning
9017, Flat bottom, polystyrene, clear

General information
This plate is one of three varieties that can be used as a Results Plate and Centrifuge
Balance Plate when running the AssayMAP Syringe Test.

Volume recommendations
Number of wells = 96
Maximum volume per well = 360 µL
Recommended overage per well = 50 µL

Resources
For information about...

See...

Product page

http://catalog2.corning.com/LifeSciences/en-US/
Shopping/
ProductDetails.aspx?categoryname=&productid=9017(Lifesciences)

Technical drawing

http://csmedia2.corning.com/LifeSciences/media/pdf/
MD_Microplate_Dimension_Sheets_96_Well.pdf

AssayMAP Bravo Platform Labware Reference Guide29

2 Labware photo reference
96 Costar 3363, PP Conical Bottom

96 Costar 3363, PP Conical Bottom
Manufacturer: Corning/Costar
Part number: 3363
Name of labware type in thesoftware:96 Costar 3363, PP Conical Bottom

General information
The Costar polypropylene conical bottom plate is a good option when attempting tominimize overage volume. The conical bottom forces liquid to drain down to a point,
which provides better accessibility for the probes of the AssayMAP Head.

Volume recommendations
Number of wells = 96
Maximum volume per well = 315 µL
Recommended overage per well = 20 µL

Resources30

For information about...

See...

Product page

http://catalog2.corning.com/LifeSciences/en-CN/
Shopping/
ProductDetails.aspx?categoryname=&productid=3363(Lifesciences)

Technical drawing

http://csmedia2.corning.com/LifeSciences/media/pdf/
MD_Microplate_Dimension_Sheets_96_Well.pdf

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

96 Eppendorf 30129300, PCR, Full Skirt, PolyPro
Manufacturer: Eppendorf
Part number: 30129300 (See Additional notes.)Name of labware type in the software: 
96 Eppendorf 30129300, PCR, Full Skirt, PolyPro

General information
This is one of three approved types of PCR plates that can be used with the Protein
Sample Prep Workbench. While plate movement is not common on AssayMAP
applications and utilities, this variety of PCR plate has a rigid skirt, which makes it easierfor the Bravo gripper to grip the plate. The elongated conical geometry of PCR styleplates, results in the smallest overage volume requirements.

Volume recommendations
Number of wells = 96
Maximum volume per well = 210 µL
Recommended overage per well = 10 µL

Additional notes
Any of the Eppendorf 96 well twin.tec skirted plates can be used, such as part numbers
0030129300, 0030129318, 0030129512, 951020401, 951020427, 951020443,
951020460, and 951020486.
The Eppendorf 0030129512 is a low-binding plate that is recommended when low
concentrations of proteins or peptides are used to minimize loss due to non-specificbinding.

Resources
For information about...

See...

Product page andtechnical drawing

https://online-shop.eppendorf.us/US-en/LaboratoryConsumables-44512/Plates-44516/Eppendorf-twintecPCR-Plates-PF-8180.html

AssayMAP Bravo Platform Labware Reference Guide31

2 Labware photo reference
96 Red PCR Insert + Eppendorf 30129300, PCR, Full Skirt

96 Red PCR Insert + Eppendorf 30129300, PCR, Full Skirt
Manufacturer and part number:•

PCR plate: Eppendorf 30129300•

Red PCR Plate Insert: Agilent G5498B#013

Name of labware type in the software:
96 Red PCR Insert + Eppendorf 30129300, PCR, Full Skirt

General information
In applications that require heating the Eppendorf 30129300 plate, place the Eppendorf
30129300 plate atop the Red PCR Insert to allow efficient heat transfer from the Peltier
Thermal Station to the Eppendorf 30129300 plate. The combination of the Eppendorf
30129300 plate stacked on the thermal insert results in a different plate height than the
Eppendorf 30129300 plate alone. Therefore, the combination is designated in the
labware selection as
96 Red PCR Insert + Eppendorf 30129300, PCR, Full Skirt.

Volume recommendations
Number of wells = 96
Maximum volume per well = 210 µL
Recommended overage per well = 10 µL

Resources32

For information
about...

See...

Eppendorf
30129300 PCR
plate

https://online-shop.eppendorf.us/US-en/LaboratoryConsumables-44512/Plates-44516/Eppendorf-twintec-PCRPlates-PF-8180.html

Red PCR Plate
InsertAgilent Customer Support

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 Eppendorf 96/1000_U-bottom, Clear PolyPro

96 Eppendorf 96/1000_U-bottom, Clear PolyPro
Manufacturer: Eppendorf
Part number: Various
(See Additional notes.)Name of labware type in the software: 
96 Eppendorf 96/1000_U-bottom, Clear PolyPro

General information
This type of deep-well plate is an option only in the In-Solution Digestion: Single Plate
v1.0 application and the Reagent Transfer v1.0 and Reagent Aliquot v1.0 utilities.
Agilent 250-µL pipette tips are required to use these plates because the AssayMAP
Cartridges and the bare probes of the AssayMAP Head cannot reach the well bottoms.

Volume recommendations
Number of wells = 96
Maximum volume per well = 1200 µL
Recommended overage per well = 25 µL

Additional notes
This plate comes in many different color options. The following catalog numbers canbe used: 951032603, 951032620, 951032662, 951032689, 951032701, 951032727,
951032760, 951032786, 951033006, and 951033103.

Resources
For information about...

See...

Product page andtechnical drawing

https://online-shop.eppendorf.us/US-en/LaboratoryConsumables-44512/Plates-44516/Eppendorf-DeepwellPlates-PF-55960.html

AssayMAP Bravo Platform Labware Reference Guide33

2 Labware photo reference
96 Eppendorf 96/500_V-bottom, Clear PolyPro

96 Eppendorf 96/500_V-bottom, Clear PolyPro
Manufacturer: Eppendorf
Part number: Various
(See Additional notes.)Name of labware type in the software: 96
Eppendorf 96/500_V-bottom, Clear PolyPro

General information
This type of deep-well plate is an option only in the In-Solution Digestion: Single Plate
v1.0 application and the Reagent Transfer v1.0 and Reagent Aliquot v1.0 utilities.
Agilent 250-µL pipette tips are required to use these plates because the AssayMAP
Cartridges and the bare probes of the AssayMAP Head cannot reach the well bottoms.

Volume recommendations
Number of wells = 96
Maximum volume per well = 700 µL
Recommended overage per well = 25 µL

Additional notes
This plate comes in many different color options, which can be used, including thefollowing part numbers: 951031801, 951031828, 951031861, 951031887, 951031909,
951031925, 951031968, 951031984, 951032204, and 951032301.

Resources34

For information about...

See...

Product page andtechnical drawing

https://online-shop.eppendorf.us/US-en/LaboratoryConsumables-44512/Plates-44516/Eppendorf-DeepwellPlates-PF-55960.html

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 Greiner 650201, U-Bottom Standard, PolyPro

96 Greiner 650201, U-Bottom Standard, PolyPro
Manufacturer: Greiner Bio-One
Part number: 650201
Name of labware type in the software:
96 Greiner 650201, U-Bottom Standard,
PolyProGeneral information
The 96 Greiner 650201 is a very common plate type for AssayMAP applications andutilities. It provides a relatively high liquid capacity, and the round bottom helps
minimize overage by forcing liquid to drain to the center of the well.

Volume recommendations
Number of Wells = 96
Maximum Volume per Well = 315 µL
Recommended Overage per Well = 20 µL

Additional notes
This plate type has two different labware definitions in the software:1
2

96 Greiner 650201, U-Bottom Standard, PolyPro
96 Greiner 650201_U-Bottom, Clear PolyPro

Only one of the two naming varieties is available in each Protein Sample Prep
Workbench application or utility.

Resources
For information about...

See...

Product page andtechnical drawing

https://shop.gbo.com/en/usa/articles/catalogue/article/
0110_0040_0120_0050/13024/

AssayMAP Bravo Platform Labware Reference Guide35

2 Labware photo reference
96 Greiner 650201_U-Bottom, Clear PolyPro

96 Greiner 650201_U-Bottom, Clear PolyPro
Manufacturer: Greiner Bio-One
Part number: 650201
Name of labware type in the software:
96 Greiner 650201_U-Bottom, Clear
PolyProGeneral information
This is a very common plate type for AssayMAP applications and utilities. It provides a
relatively high liquid capacity, and the round bottom helps minimize overage by forcing
liquid to drain to the center of the well.

Volume recommendations
Number of Wells = 96
Maximum Volume per Well = 315 µL
Recommended Overage per Well = 20 µL

Additional notes
This plate type has two different labware definitions in the software:1
2

96 Greiner 650201_U-Bottom, Clear PolyPro
96 Greiner 650201, U-Bottom Standard, PolyPro

Only one of the two naming varieties is available in each Protein Sample Prep
Workbench application or utility.

Resources36

For information about...

See...

Product page andtechnical drawing

https://shop.gbo.com/en/usa/articles/catalogue/article/
0110_0040_0120_0050/13024/

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 Greiner U-Bottom Thermal Insert + Greiner 650201_U-Bottom, Clear PolyPro

96 Greiner U-Bottom Thermal Insert + Greiner 650201_U-Bottom,
Clear PolyPro
Manufacturer and part number:•

Greiner plate: Greiner Bio-One 650201•

96 Greiner U- or V-Bottom Insert:
Agilent G5498B#126

Name of labware type in the software:
96 Greiner U-Bottom Thermal Insert +Greiner 650201_U-Bottom, Clear PolyPro

General information
In applications that require heating the Greiner 650201 plate, place the Greiner 650201
plate atop the 96 Greiner U-Bottom Thermal Insert to allow efficient heat transfer fromthe Peltier Thermal Station to the Greiner 650201 plate. The combination of the Greiner
650201 plate stacked on the thermal insert results in a different plate height than the
Greiner 650201 plate alone. Therefore, the combination is designated in the labware
selection as
96 Greiner U-Bottom Thermal Insert + Greiner 650201_U-Bottom, Clear PolyPro

Volume recommendations
Number of Wells = 96
Maximum Volume per Well = 315 µL
Recommended Overage per Well = 20 µL

Resources
For information about...

See...

Greiner platehttps://shop.gbo.com/en/usa/articles/catalogue/article/
0110_0040_0120_0050/13024/

Thermal Insert

https://www.agilent.com/en/products/automationsolutions/protein-sample-preparation/assaymap-bravoplatform

AssayMAP Bravo Platform Labware Reference Guide37

2 Labware photo reference
96 Greiner 650207_U-Bottom, White PolyPro

96 Greiner 650207_U-Bottom, White PolyPro
Manufacturer: Greiner Bio-One
Part number: 650207
Name of labware type in the software: 
96 Greiner 650207_U-Bottom, White PolyPro

General information
This is a very common plate type for AssayMAP applications and utilities. It provides a
relatively high liquid capacity, and the round bottom helps minimize overage by forcing
liquid to drain to the center of the well. The opaque white coloration also provides
additional light protection for sensitive reagents.

Volume recommendations
Number of wells = 96
Maximum volume per well = 315 µL
Recommended overage per well = 20 µL

Resources38

For information about...

See...

Product page andtechnical drawing

https://shop.gbo.com/en/usa/articles/catalogue/article/
0110_0040_0120_0050/13035/

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 Greiner 651201_V-Bottom, Clear PolyPro

96 Greiner 651201_V-Bottom, Clear PolyPro
Manufacturer: Greiner Bio-One
Part number: 651201
Name of labware type in the software: 
96 Greiner 651201_V-Bottom, Clear
PolyProGeneral information
The Greiner polypropylene V-bottom plate is a good option when attempting tominimize overage volume. The conical bottom forces liquid to drain down to a point,providing good accessibility for the bare probes of the AssayMAP Head or for the
cartridges.

Volume recommendations
Number of wells = 96
Maximum volume per well = 315 µL
Recommended overage per well = 20 µL

Resources
For information about...

See...

Product page andtechnical drawing

https://shop.gbo.com/en/usa/articles/catalogue/article/
0110_0040_0120_0050/13071/

AssayMAP Bravo Platform Labware Reference Guide39

2 Labware photo reference
96 Greiner V-Bottom Thermal Insert + Greiner 651201, V-Bottom, Clear PolyPro

96 Greiner V-Bottom Thermal Insert + Greiner 651201, V-Bottom,
Clear PolyPro
Manufacturer and part number:•

Greiner plate: Greiner Bio-One 651201•

96 Greiner U- or V-Bottom Insert:
Agilent G5498B#126

Name of labware type in the software:
96 Greiner V-Bottom Thermal Insert +Greiner 651201, V-Bottom, Clear PolyPro

General information
In applications that require heating the Greiner 651201 plate, you place the Greiner
651201 plate atop the 96 Greiner V-Bottom Thermal Insert to allow efficient heattransfer from the Peltier Thermal Station to the Greiner 651201 plate. The combinationof the Greiner 651201 plate stacked on the thermal insert results in a different plateheight than the Greiner 651201 plate alone. Therefore, the combination is designated in
the labware selection as
96 Greiner V-Bottom Thermal Insert + Greiner 651201, V-Bottom, Clear PolyPro.

Volume recommendations
Number of wells = 96
Maximum volume per well = 315 µL
Recommended overage per well = 20 µL

Resources40

For information about...

See...

Greiner platehttps://shop.gbo.com/en/usa/articles/catalogue/article/
0110_0040_0120_0050/13071/

Thermal Insert

https://www.agilent.com/en/products/automationsolutions/protein-sample-preparation/assaymap-bravoplatform
AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 Greiner 652270, PCR, Full Skirt, PolyPro

96 Greiner 652270, PCR, Full Skirt, PolyPro
Manufacturer: Greiner Bio-One
Part number: 652270
Name of labware type in the software: 
96 Greiner 652270, PCR, Full Skirt, PolyPro

General information
This is one of three approved types of PCR plates that can be used with the Protein
Sample Prep Workbench. The elongated conical geometry of PCR style plates, resultsin the smallest overage volume requirements.

Volume recommendations
Number of wells = 96
Maximum volume per well = 210 µL
Recommended overage per well = 10 µL

Resources
For information about...

See...

Product page andtechnical drawing

https://shop.gbo.com/en/usa/articles/catalogue/
article/5_0060_0030_0040/13115/

AssayMAP Bravo Platform Labware Reference Guide41

2 Labware photo reference
96 Red PCR Insert + Greiner 652270, PCR, Full Skirt, PolyPro

96 Red PCR Insert + Greiner 652270, PCR, Full Skirt, PolyPro
Manufacturer and part number:•

PCR plate: Greiner Bio-One 652270•

Red PCR Plate Insert: Agilent G5498B#013

Name of labware type in the software:
96 Red PCR Insert + Greiner 652270, PCR, Full Skirt, PolyPro

General information
In applications that require heating the Greiner 652270 plate, place the Greiner 652270
plate atop the Red PCR Insert to allow efficient heat transfer from the Peltier Thermal
Station to the Greiner 652270 plate. The combination of the Greiner 652270 platestacked on the thermal insert results in a different plate height than the Greiner 652270
plate alone. Therefore, the combination is designated in the labware selection as
96 Red PCR Insert + Greiner 652270, PCR, Full Skirt, PolyPro.

Volume recommendations
Number of wells = 96
Maximum volume per well = 210 µL
Recommended overage per well = 10 µL

Resources

42

For information about...

See...

Greiner platehttps://shop.gbo.com/en/usa/articles/catalogue/article/
5_0060_0030_0040/13115/

Red PCR Plate Insert

https://www.agilent.com/en/products/automationsolutions/protein-sample-preparation/assaymap-bravoplatform

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 Greiner 655101 PS Clr Rnd Well Flat Btm

96 Greiner 655101 PS Clr Rnd Well Flat Btm
Manufacturer: Greiner Bio-One
Part number: 655101
Name of labware type in the software: 
96 Greiner 655101 PS Clr Rnd Well Flat Btm

General information
This plate is one of three varieties that can be used as a Results Plate and Centrifuge
Balance Plate when running an AssayMAP Syringe Test.

Volume recommendations
Number of wells = 96
Maximum volume per well = 360 µL
Recommended overage per well = 50 µL

Additional notes
Two of these plates are included with the AssayMAP Syringe Test kit.

Resources
For information about...

See...

Product page andtechnical drawing

https://shop.gbo.com/en/usa/articles/catalogue/article/
0110_0040_0120_0010/13243/

AssayMAP Bravo Platform Labware Reference Guide43

2 Labware photo reference
96 Greiner 675801, Half Area, Flat Bottom, UV Star

96 Greiner 675801, Half Area, Flat Bottom, UV Star
Manufacturer: Greiner Bio-One
Part Number: 675801
Name of labware type in the software: 
96 Greiner 675801, Half Area, Flat Bottom, UV
Star

General information
This plate is generally used for the purification and normalization applications, when
protein concentration will need to be determined using protein A280. The flat bottom on
this Greiner plate results in a very high overage volume, which is an importantconsideration when transitioning from this labware into another type.

Volume recommendations
Number of wells = 96
Maximum volume per well = 190 µL
Recommended overage per well = 25 µL

Resources

44

For information about...

See...

Product page andtechnical drawing

https://shop.gbo.com/en/usa/articles/catalogue/article/
5_0060_0020_0020_0040/13517/

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 Nunc 269620, Flat Bottom, Polystyrene

96 Nunc 269620, Flat Bottom, Polystyrene
Manufacturer: Nunc (Thermo-Fisher)
Part number: 269620
Name of labware type in the software: 
96 Nunc 269620, Flat Bottom, Polystyrene

General information
This plate is one of three varieties that can be used as a Results Plate and Centrifuge
Balance Plate when running the AssayMAP Syringe Test utility.

Volume recommendations
Number of wells = 96
Maximum volume per well = 360 µL
Recommended overage per well = 50 µL

Resources
For information about...

See...

Product page andtechncial drawing

http://www.thermoscientific.com/en/products/96-wellmicroplates.html

AssayMAP Bravo Platform Labware Reference Guide45

2 Labware photo reference
96 Thermo Matrix 3732, V-bottom, 0.75ml Storage Tubes

96 Thermo Matrix 3732, V-bottom, 0.75ml Storage Tubes
Manufacturer: ThermoFisher ScientificPart number: 3732
Name of labware type in the software: 
96 Thermo Matrix 3732, V-bottom, 0.75ml
Storage Tubes

General information
This storage tube rack is one of three varieties that can be used for the following:•

Diluent reservoir and Serial Dilution plate for the Serial Dilution utility•

Source and Destination plates for the Reformatting utility

Volume recommendations
Number of wells = 96
Maximum volume per tube = 0.75 mL
Recommended overage per well = 20 µL

Resources

46

For information about...

See...

Product pagehttps://www.thermofisher.com/order/catalog/product/
3710

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
96 Thermo Matrix 3735, V-bottom, 500µl Storage Tubes

96 Thermo Matrix 3735, V-bottom, 500µl Storage Tubes
Manufacturer: ThermoFisher ScientificPart number: 3735
Name of labware type in the software: 
96 Thermo Matrix 3735, V-bottom, 500µl
Storage Tubes

General information
This storage tube rack is one of three varieties that can be used for the following:•

Diluent reservoir and Serial Dilution plate for the Serial Dilution utility•

Source and Destination plates for the Reformatting utility

Volume recommendations
Number of wells = 96
Maximum volume per tube = 500 µL
Recommended Overage per Well = 20 µL

Resources
For information about...

See...

Product pagehttps://www.thermofisher.com/order/catalog/product/
3710

AssayMAP Bravo Platform Labware Reference Guide47

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96 Thermo Matrix 3744, V-bottom, 500µl ScrewTop Storage Tubes

96 Thermo Matrix 3744, V-bottom, 500µl ScrewTop Storage
Tubes
Manufacturer: ThermoFisher ScientificPart number: 3744
Name of labware type in the software: 
96 Thermo Matrix 3744, V-bottom, 500µl
ScrewTop Storage Tubes

General information
This storage tube rack is one of three varieties that can be used for the following:•

Diluent reservoir and Serial Dilution plate for the Serial Dilution utility•

Source and Destination plates for the Reformatting utility

Volume recommendations
Number of wells = 96
Maximum volume per tube = 500 µL
Recommended Overage per Well = 20 µL

Resources

48

For information about...

See...

Product pagehttps://www.thermofisher.com/order/catalog/product/
3710

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96 Waters 186005837, Clear PolyPro

96 Waters 186005837, Clear PolyPro
Manufacturer: Waters
Part number: 186005837
Name of labware type in the software: 
96 Waters 186005837, Clear PolyPro

General information
This plate can be used for the following:•

Reagent Aliquot, Reagent Transfer, Reformatting, and Serial Dilution utilities•

In-Solution Digestion: Single Plate application

Volume recommendations
Number of wells = 96
Maximum volume per tube = 500 µL

Resources
For information about...

See...

Product page

http://www.waters.com/waters/
partDetail.htm?partNumber=186005837&locale=en_US

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96AM 250 µL Tip Loading Station

96AM 250 µL Tip Loading Station
Manufacturer: Agilent Technologies
Part number: G5409-20025
Name of labware type in the software: 
96AM 250 µL Tip Loading Station

General information
The Agilent 96AM Cartridge & Tip Seating Station accessory is included with all
AssayMAP Bravo purchases. This accessory is required when seating pipette tips on
the probes of the AssayMAP Bravo head.

Volume recommendations
Number of wells = 96
Maximum volume per well = Not applicable
Recommended overage per well = Not applicable

Additional notes
This accessory has two labware names in the software:

1

96AM 250 µL Tip Loading Station, which is used for seating tips on the AssayMAP
Bravo head

2

96AM Cartridge Seating Station, which is used for seating cartridges on the
AssayMAP Bravo head

The engraving, 96AM 250uL TIP LOADING STATION, appears on the side of theaccessory.

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96AM Cartridge Rack and Receiver Plate

96AM Cartridge Rack and Receiver Plate
Manufacturer: Agilent Technologies
Part number: See the Agilent AssayMAP
Cartridge Selection Guide.
Name of labware type in the software: 
96AM Cartridge Rack and Receiver Plate

General information
The AssayMAP Cartridges are shipped in the 96AM Cartridge Rack and Receiver Plate.
The labware consists of two distinct pieces: the blue cartridge rack and the white
receiver plate. The cartridges are transferred from this labware into the 96AM Cartridge
Seating Station before use on the AssayMAP Bravo Platform.

Volume recommendations
Number of wells = 96
Maximum volume per well = Not applicable
Recommended overage per well = Not applicable

Additional notes
Partial plate operations with the head positioned at an offset to the deck location are
possible with this piece of labware, for example, using the Cartridge Transfer utility.
One known issue is that the AssayMAP Bravo head might pick up the cartridge rack
unintentionally while attempting to seat a full rack of 96 cartridges. When using a fullcartridge rack, pay close attention to the AssayMAP Bravo head during a run of the
Cartridge Transfer utility.

Resources
For information about...

See...

Product page

http://www.agilent.com/cs/library/selectionguide/public/
5991-4863EN.pdf

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96AM Receiver Plate

96AM Receiver Plate
Manufacturer: Agilent Technologies
Part number: Not applicable
Name of labware type in the software: 96AM
Receiver Plate

General information
The 96AM Receiver Plate is the bottom piece of the 96AM Cartridge Rack and Receiver
Plate. The cartridge rack stacks on the receiver plate during shipment. The cartridges
are transferred from the 96AM Cartridge Rack and Receiver plate into the96AM
Cartridge Seating Station for automation tasks on the AssayMAP Bravo Platform. If the
cartridge rack is used during automation tasks, the receiver plate is required forsupport.

Volume recommendations
Number of wells = 96
Maximum volume per well = 200 µL when a cartridge is present
Recommended overage per well = Not applicable

Additional notes
The wells of the receiver plate can be filled with 200 µL of liquid and used as atemporary storage location for used cartridges, for example, after running theimmobilization protocol and before using the cartridges in the affinity purification
protocol. When the cartridges are put in the blue rack in a position where the well below
it has 200 µL of liquid, the cartridge tips will be submerged in the liquid and thereby
avoid drying out. Cartridges should never be allowed to dry out.

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96AM Cartridge Seating Station

96AM Cartridge Seating Station
Manufacturer: Agilent Technologies
Part number: G5409-20025
Name of labware type in the software: 
96AM Cartridge Seating Station

General information
The Agilent 96AM Cartridge & Tip Seating Station accessory is included with all
AssayMAP Bravo purchases. This accessory is the “labware” used for seating the
cartridges on the probes of the AssayMAP Bravo head.

Volume recommendations
Number of wells = 96
Maximum volume per well = Not applicable
Recommended overage per well = Not applicable

Additional notes
This accessory has two labware type names in the software:

1

96AM Cartridge Seating Station, which is used for seating cartridges on the
AssayMAP Bravo head

2

96AM 250 µL Tip Loading Station, which is used for seating tips on the AssayMAP
Bravo head

The engraving, 96AM 250uL TIP LOADING STATION, appears on the side of theaccessory.

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96AM Tip Wash Station

96AM Tip Wash Station
Manufacturer: Agilent Technologies
Part number: G5498B#057 
Name of labware type in the software: 
96AM Tip Wash Station

General information
The wash station is installed on deck location 1 of the AssayMAP Bravo Platform. The
wash station is used for internally and externally washing syringes, cartridges, andpipette tips. The wash station also serves as a waste collection location.

Volume recommendations
Number of wells = 96
Maximum volume per well = Not applicable
Recommended overage per well = Not applicable

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96 V11 Manual Fill Reservoir

96 V11 Manual Fill Reservoir
Manufacturer: Agilent Technologies
Part Number: G5498B#049(Internal part number: 08105-001)

Name of labware type in the software: 
96 V11 Manual Fill Reservoir, 
96 V11 Reservoir (Manual Fill)

General information
The 96 V11 Manual Fill Reservoir is an open-bath reservoir that can be used for fullplate runs of 96 samples or cartridges.

Volume recommendations
Number of wells = 1
Maximum volume per well = 150 mL
Recommended overage per well = 35 mL

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96 V11 LT 250 Tip Box 19477.002

96 V11 LT 250 Tip Box 19477.002
Manufacturer: Agilent Technologies
Part number: 19477-002
Name of labware type in the software: 
96 Vll LT 250 Tip Box

General information
The pipette tips are transferred from the tip box into the 96AM 250 µL Tip Loading
Station before use in an AssayMAP application or utility.

Volume recommendations
Number of wells = 96
Maximum volume per well = 140 µL can be pulled into a mounted tip before the excess
fluid overflows into the syringe.
Recommended overage per well = Not applicable

Additional notes
The 96AM 250 µL Tip Loading Station is required to seat the pipette tips on the Bravo
96AM Head.

Resources

56

For information about...

See...

Ordering

https://www.chem.agilent.com/store/

AssayMAP Bravo Platform Labware Reference Guide2 Labware photo reference
Bravo Plate Riser

Bravo Plate Riser
Vendor: Agilent Technologies
Part number: G5498B#061
Name of labware type in the software: Plate Riser

General information
The Bravo plate riser is required for certain AssayMAP applications and utilities where
pipette tips are used for the liquid handling. The 2.84-cm tall riser lifts the labware to aheight to the Bravo 96AM Head to be offset when using pipette tips. For example, theNormalization, Serial Dilution, and Reformatting utilities require a pair of plate risers.
One riser is placed on the platepad under the source plate and one riser is placed on theplatepad under the destination plate.

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Universal Lid PS Black

Universal Lid PS Black
Vendor: Agilent Technologies
Part number: 200858-100
Name of labware type in the software: Plate Lidded

General information
Lidded plates, not the lid by itself, are allowed for the In-Solution Digestion: Single-Plate
v1.0 application. If you plan to use a lidded plate, ensure that you use this universal
microplate lid, which is the only approved lid type.

Additional notes
Several different vendors offer the exact same lid under different part numbers, forexample, Phenix Research ML-5009 or ML-5010, E&K Scientific EK-2079, EK-2080, orEK-2081.
Three color varieties are available for this lid: opaque black, opaque white, andtransparent. All three colors are acceptable for use with the In-Solution Digestion:
Single-Plate v1.0 application.

Resources

58

For information about...

See...

Ordering

https://www.chem.agilent.com/store/

AssayMAP Bravo Platform Labware Reference Guide

In This Book
This guide provides a labware reference for
the AssayMAP Protein Sample Prep
Workbench, including• Labware options and default settings• Labware photo reference

www.agilent.com
Agilent Technologies, Inc. 2019
January 2019

*G5496-90018*
G5496-90018 Revision C