The MiniSeq™ System.
Explore the possibilities.
Discover demonstrated workflows for oncology applications.

Let your work flow with Illumina NGS.
The MiniSeq System delivers powerful and cost-effective methods for molecularprofiling in a highly accurate benchtop solution. Its convenient and streamlined
library-to-results workflow enables rapid sequencing of both DNA and RNA for analysis
of a single gene or entire pathways in 1 run. Supported by a full suite of Illumina library
preparation solutions, the MiniSeq System features an intuitive, touch-screen userinterface, integrated data analysis, and a small footprint. Every aspect is designed foreasy, everyday use. Finally, next-generation sequencing (NGS) that fits your budget,your bench, and your research needs.

NGS has transformed the field of cancer research. In the past, iterative tests
with technologies that examined only 1 gene at a time exhausted available tissue
samples and delayed the analysis of mutations and somatic variants. Now with NGS,
researchers can assess multiple genes in a single test, with predefined panels ofexpert-selected content or custom-tailored panels. NGS is an efficient method forgaining powerful and reliable insights into a range of genes in a single, streamlined
workflow. Explore the many demonstrated workflows for oncology applications.

For Research Use Only. Not for use in diagnostic procedures.

Solid tumor profiling

Hematological cancer profiling

Germline testing

Broad molecular profilingTruSight® Tumor 15
Research common somatic variants in solid tumor samples.
The TruSight Tumor 15 workflow provides a comprehensive assessment of 15 genes that are commonly mutated in solidtumors. It accurately analyzes low-frequency variants from 20 ng of starting DNA and is optimized for formalin-fixed,paraffin-embedded (FFPE) tumor tissue. Featuring a rapid workflow that can be easily integrated into lab procedures,
it offers a single assay for accurate, economical, and rapid analysis of solid tumors.
Genes included
AKT1, BRAF, EGFR, ERBB2, FOXL2, GNA11, GNAQ, KIT, KRAS, MET, NRAS, PDGFRA, PIK3CA, RET, TP53

TruSight Tumor 15 Kit
www.illumina.com/oncologypanelHighlights• Discover a complete workflow with detailed QC
steps and simple, predefined variant report.
• Experience rapid turnaround—3.5 hours of handson time; DNA to data in approximately 36 hours.
• Utilize somatic variants selected from relevant
industry guidelines, key opinion leaders, andpharmaceutical researchers.
• Achieve accurate somatic variant analysis of 5%
allele frequency with as little as 20 ng DNA fromFFPE tissue samples.

On System: Local Run Manager—TruSight Tumor 15
On Cloud: BaseSpace® Platform—TruSight Tumor 15
On Site:BaseSpace® Platform—TruSight Tumor 15
www.illumina.com/basespace

For Research Use Only. Not for use in diagnostic procedures.

Solid tumor profilingTruSeq® Custom Amplicon Low Input
Dual Strand
Screen FFPE samples for variants in custom gene sets.
The TruSeq Custom Amplicon Low Input Dual Strand workflow is a valuable extension to the TruSeq Custom Amplicon
Low Input Kit. The workflow interrogates each complementary strand with a mirror probe design. This can further enhance
the ability to filter actual variants from systemic “noise,” providing confidence in variant identification. Overcome sequencing
artifacts caused by DNA damage such as deamination from FFPE and oxidation, and sequence context challenges,including repeats and base-read errors.

TruSeq Custom Amplicon Low Input Kit
www.illumina.com/ampliconseq

Highlights• Experience a completely customizable solution
using the DesignStudio™ Tool for your genes andtargets of interest.
• Leverage Illumina Concierge for personal design
assistance and optimization.
• Achieve accurate variant detection from as little
as 20 ng of DNA.
• Sequence up to 1536 amplicons in 2 poolreactions using a simple workflow.
• Rely on a fully supported, optimized workflow
solution that includes simple onboard or
cloud-based data analysis.

On System: Local Run Manager—Amplicon DS
On Cloud: BaseSpace Platform—Amplicon DS
On Site:BaseSpace Platform—Amplicon DS
VariantStudio
www.illumina.com/basespace

For Research Use Only. Not for use in diagnostic procedures.

Solid tumor profilingTruSight Myeloid
Screen key genes in myeloid malignancies.
The TruSight Myeloid workflow covers 15 full genes (exons only) and key oncogenic hotspots of 39 additional genes,
providing a comprehensive assessment of the key genes involved in myeloid malignancies in a single test. The result is an
accurate, cost-effective solution for profiling common myeloid neoplasms.
Genes included
ABL1, ASXL1, ATRX, BCOR, BCORL1, BRAF, CALR, CBL, CBLB, CBLC, CDKN2A, CEBPA, CSF3R, CUX1, DNMT3A,ETV6/TEL, EZH2, FBXW7, FLT3, GATA1, GATA2, GNAS, HRAS, IDH1, IDH2, IKZF1, JAK2, JAK3, KDM6A, KIT, KRAS,MLL, MPL, MYD88, NOTCH1, NPM1, NRAS, PDGFRA, PHF6, PTEN, PTPN11, RAD21, RUNX1, SETBP1, SF3B1,SMC1A, SMC3, SRSF2, STAG2, TET2, TP53, U2AF1, WT1, ZRSR2Highlights• Leverage expert consortia-selected content
targeting 54 genes mutated frequently in
myeloid malignancies.
• Benefit from a single workflow that includes library
preparation, sequencing, data analysis, anddata annotation.
• Assess multiple genes simultaneously withhigh accuracy and sensitivity—efficiently andcost effectively.

On System: Local Run Manager—Amplicon
On Cloud: BaseSpace Platform—TruSeq Amplicon
On Site:BaseSpace Platform—TruSeq Amplicon
VariantStudio
www.illumina.com/basespace

TruSight Myeloid
www.illumina.com/trusightmyeloid

For Research Use Only. Not for use in diagnostic procedures.

Hematological cancer profilingTruSight Cancer
Broadly screen for variants linked to cancer.
The TruSight Cancer workflow targets 94 genes associated with both common (eg, breast, colorectal) and rare cancers and284 single nucleotide polymorphisms (SNPs) found to correlate with cancer through genome-wide association studies (GWAS).
Conduct comprehensive evaluation of genes that contain genetic variants linked to a predisposition for cancer.
Genes included
AIP, ALK, APC, ATM, BAP1, BLM, BMPR1A, BRCA1, BRCA2, BRIP1, BUB1B, CDC73, CDH1, CDK4, CDKN1C, CDKN2A, CEBPA,CEP57, CHEK2, CYLD, DDB2, DICER1, DIS3L2, EGFR, EPCAM, ERCC2, ERCC3, ERCC4, ERCC5, EXT1, EXT2, EZH2, FANCA,FANCB, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCL, FANCM, FH, FLCN, GATA2, GPC3, HNF1A, HRAS, KIT, MAX,MEN1, MET, MLH1, MSH2, MSH6, MUTYH, NBN, NF1, NF2, NSD1, PALB2, PHOX2B, PMS1, PMS2, PRF1, PRKAR1A, PTCH1, PTEN,
RAD51C, RAD51D, RB1, RECQL4, RET, RHBDF2, RUNX1, SBDS, SDHAF2, SDHB, SDHC, SDHD, SLX4, SMAD4, SMARCB1, STK11,SUFU, TMEM127, TP53, TSC1, TSC2, VHL, WRN, WT1, XPA, XPC

TruSight Cancer
www.illumina.com/products/trusight_cancer.html

Highlights• Capture genes associated with a predisposition
for cancer with content selected by the Instituteof Cancer Research, London.
• Preserve precious samples, achieving data quality
with as little as 50 ng of DNA.
• Benefit from a fast, simple workflow compatible
with TruSight Rapid Capture Kits that enables
library prep and enrichment in 1.5 days.

On System: Local Run Manager—DNA Enrichment
On Cloud: BaseSpace Platform—Isaac/BWA Enrichment
On Site:BaseSpace Platform—Isaac/BWA Enrichment
VariantStudio
www.illumina.com/basespace

For Research Use Only. Not for use in diagnostic procedures.

Germline testingTruSeq Custom Amplicon Low Input
Screen for variants in custom gene sets.
The TruSeq Custom Amplicon Low Input workflow is a highly targeted approach offering unparalleled efficiency indiscovering, validating, and screening genetic variants, even from limited and challenging samples, such as FFPE.
Interrogate your specific areas of interest with deep sequencing for higher coverage and greater resolution for highly
accurate results.

Highlights• Achieve accurate variant detection from as little
as 10 ng of DNA.
• Experience a completely customizable solution
using the DesignStudio Tool for your genes andtargets of interest.
• Leverage Illumina Concierge for additional
design assistance and optimization.
• Sequence up to 1536 amplicons in a single
reaction using a simple workflow.
• Rely on a fully supported, optimized workflow
solution that includes simple onboard orcloud-based data analysis.

On System: Local Run Manager—Amplicon
On Cloud: BaseSpace Platform—TruSeq Amplicon
On Site:BaseSpace Platform—TruSeq Amplicon
VariantStudio
www.illumina.com/basespace

TruSeq Custom Amplicon Low Input
www.illumina.com/ampliconseq

For Research Use Only. Not for use in diagnostic procedures.

Germline testingTruSight RNA Pan-Cancer
Screen both solid and hematological cancers.
The TruSight RNA Pan-Cancer workflow enables analysis of gene fusions, variants, and gene expression changes in1385 genes, providing a comprehensive view of the known functionally relevant changes occurring in cancer. It provides asensitive, reproducible, and economical solution for studies of expression dynamics and functional mechanisms in cancer.
Genes included
ABCC3, ABI1, ABL1, ABL2, ABLIM, ACACA, ACE, ACER, ACKR3, ACSBG1, ACSL3, ACSL6, ACVR1B, ACVR1C,ACVR2A, ADD3, ADM, AFF1, AFF3, AFF4, AGR3, AHCYL1, AHI1, AHR, AHRR, AIP, AK2, AK5, AKAP12, AKAP6, AKAP9,AKR1C3, AKT1, AKT2, AKT, ALDH1A1, ALDH2, ALDOC, ALK, AMER1, AMH, ANGPT1, ANKRD28, ANLN, APC, APH1A,APLP2, APOD. For a complete list, visit www.illumina.com/products/trusight-rna-pan-cancer-panel.

Highlights• Access gene expression information andfusion discovery with known and novel genefusion partners.
• Streamline your workflow with an optimized,low-input protocol for a wide range of sample
types including FFPE.

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TruSight RNA Pan-Cancer
www.illumina.com/products/trusight-rna-pan-cancer-panel.html

On Cloud: BaseSpace Platform—RNA Core
On Site: BaseSpace Platform—RNA Core
VariantStudio
www.illumina.com/basespace

For Research Use Only. Not for use in diagnostic procedures.

Broad molecular profiling

Committed to your success with services,training, and personalized consulting.
Rely on accurate and expedient solutions to match your evolving needs. Whether you are just beginning to evaluate your NGS options,or you are an experienced NGS user looking to access more strategic consulting alternatives, we have services to support you every
step of the way.
Our offerings are flexible and customizable to fit your lab’s unique needs.

Product care services

Illumina University training• Tiered service plans, plus add-on options• Compliance and on-demand services to meet yourevolving needs• Instructor-led training for the entire workflow• Online courses• Webinars

Personalized consulting• Bioinformatics guidance for ease of adoption• Proof-of-concept services for instrument and library
prep testing• Concierge services for design assistance andproduct optimization
For Research Use Only. Not for use in diagnostic procedures.

Cluster generation and sequencing.
MiniSeq System High-Output Kit*

MiniSeq System Mid-Output Kit*

Cycles

OutputCycles

Output300

7.5 Gb

300

2.4 Gb150

3.75 Gb

75

1.875 Gb

Reads passing filter
MiniSeq System High-Output Kit

MiniSeq System Mid-Output Kit

Single reads

Up to 25 million

Single reads

Up to 8 million

Paired-end reads

Up to 50 million

Paired-end reads

Up to 16 million* Install specifications based on the Illumina PhiX Control Library at supported cluster densities (between 129 and 165 k/mm2 clusters passing filter). Actual performance parameters mayvary based on sample type, sample quality, and clusters passing filter. All MiniSeq System library prep kits are paired-end compatible.
For Research Use Only. Not for use in diagnostic procedures.

Industry-leading solutions.
A community of support.
From library prep, arrays, and sequencing to informatics, Illumina genomic solutions empower
researchers and clinical researchers across the globe to find the answers they seek.
When you join the Illumina community, you become part of a dynamic scientific movement thatincludes thousands of researchers and industry thought leaders. Throughout the year, we hostuser group meetings, symposia, consortia, online forums, and other initiatives—all designed tobring the best minds together to share ideas and advance science.
In addition to on-site training, ongoing support, and phone consults, we offer webinars and courses
at various Illumina locations. We’re here with all the resources you need to accelerate progress.

The MiniSeq System is a small, robust sequencer, perfect for everyday sequencing. Incorporating
the latest advancements in sequencing by synthesis (SBS) chemistry, the flexible MiniSeq Systemfeatures push-button operation and a streamlined library-to-results workflow.
Learn more about the MiniSeq System at www.illumina.com/miniseq.

A global genomics leader, Illumina provides complete next-generation sequencing workflow solutions to the basic andtranslational research communities. Illumina technology is responsible for generating more than 90% of the world’s
sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in the fields of oncology,
reproductive health, genetic disease, microbiology, agriculture, and forensic science.
*Data calculations on file. Illumina, Inc., 2015.
Illumina • 1.800.809.4566 toll-free (US) • +1.858.202.4566 tel • techsupport@illumina.com • www.illumina.com
For Research Use Only. Not for use in diagnostic procedures.
© 2016 Illumina, Inc. All rights reserved. Illumina, BaseSpace, DesignStudio, MiniSeq, TruSeq, TruSight, and the pumpkin orange color are trademarks of Illumina, Inc. and/or
its affiliate(s) in the US and/or other countries. All other names, logos, and other trademarks are the property of their respective owners.
Pub. No. 770-2015-050 Current as of 07 January 2016