SPring-8/SACLA ฼⏝◊✲ᡂᯝ㞟
2016A1753                       Section
Section BBL46XU࣐࢖ࢡࣟࣅ࣮࣒ X ⥺ᩓ஘ἲࢆ⏝࠸ࡓࣄࢺ⓶⭵ゅᒙᵓ㐀ࡢ῝ࡉ᪉ྥゎᯒ IIThe Depth Direction Structural Analysis of Human Stratum Corneum
Using Microbeam X-ray Scattering: Part II.
ஂ⡿ ༟ᚿ a, ᑠ㔝ᑿ ಙ a, ⏣ᮧ ಇ⣫ a, ඵᮌ ┤ே b, ඵ⏣ ୍㑻 c
Takuji Kumea, Makoto Onooa, Toshihiro Tamuraa, Naoto Yagib, Ichiro Hattacaⰼ⋤ᰴᘧ఍♫, b㻔බ㈈㻕㧗㍤ᗘග⛉Ꮫ◊✲䝉䞁䝍䞊, c㻔බ㈈㻕ྡྂᒇ⏘ᴗ⛉Ꮫ◊✲ᡤ aKao Corporation, bJASRI, cNISRI

௨๓ࡢ X ⥺ᩓ஘ἲࢆ⏝࠸ࡓ⓶⭵ゅᒙࡢᵓ㐀ゎᯒ࡛ࡣࠊ᭱እᒙ࡛࠶ࡿゅᒙࡢࡳࡢࢩ࣮ࢺࢆ⏝࠸࡚ࠊゅᒙࡢ㞟ྜయ࠿ࡽࡢᖹᆒ᝟ሗࢆྲྀࡾᢅࡗ࡚࠸ࡓࠋᡃࠎࡣ in situ ࡟㏆࠸᮲௳࡛ࡢゅᒙࡢ῝ࡉ᪉ྥࡢᵓ㐀ゎᯒἲ࡜ࡋ࡚ࠊゅᒙ௨ୗࡢ⾲⓶ࠊ┿⓶➼ࡶྵࡴࣄࢺ⓶⭵ࢩ࣮ࢺࢆ⏝࠸࡚࣐࢖ࢡࣟࣅ࣮࣒ X ⥺ࢆࢫ࢟ࣕࣥࡋ࡚࠸ࡃ᪉ἲ࡛⓶⭵ୖࡢゅᒙ࠿ࡽࡢᑠゅ࣭ᗈゅᩓ஘ീࢆᚓࡿࡇ࡜࡟ᡂຌࡋࡓࠋࡇࢀ࡟ࡼࡾゅᒙෆ㒊ࡢ῝ࡉ᪉ྥࡢᵓ㐀ኚ໬ࢆゎᯒࡍࡿࡇ࡜࡛ࠊ⓶⭵࡬ࡢ๣ࡢస⏝ࠊᾐ㏱ຠᯝ➼ࡢヲ⣽࡞▱ぢࡀᚓࡽࢀࡿࡇ࡜ࡀᮇᚅࡉࢀࡿࠋ
࣮࣮࢟࣡ࢻ㸸 Human stratum corneum, Surfactant, microbeam X-ray scattering, Keratin fibril structure,
Intercellular lipid lamella structure⫼ᬒ࡜◊✲┠ⓗ㸸໬⢝ရ࣭㤶⢝ရ࡟ࡣࠊὙί࡛⓶⭵ࢆΎ₩࡟ಖࡘ࣭᭷ຠᡂศࢆຠᯝⓗ࡟⓶⭵࡟స⏝ࡉࡏࡿ➼ࡢ┠ⓗ࡛ᵝࠎ࡞⏺㠃άᛶ๣ࡲࡓࡣ⤒⓶྾཰ಁ㐍๣࡞࡝ࡢ๣ࡀ㓄ྜࡉࢀ࡚࠸ࡿࠋࡲࡓࠊ⾰㢮࣭㣗ჾ⏝Ὑ๣➼ࡢ⏕ά⏝ရ୰࡟ࡶởࢀࢆⴠ࡜ࡍ┠ⓗ࡛ᵝࠎ࡞⏺㠃άᛶ๣ࡀ฼⏝ࡉࢀ࡚࠸ࡿࠋࡇࢀࡽࡢ๣ࡣࠊࡑࡢ✀㢮ࡸฎ⌮᮲௳࡟ࡼࡗ࡚ࡣ⓶⭵ࡢゅᒙᵓ㐀ࢆኚ໬ࡉࡏࠊゅᒙᶵ⬟㸦ಖ‵ࡸࣂࣜ࢔ᶵ⬟㸧ࡢపୗࢆᣍࡃᜍࢀࡀ࠶ࡿࠋࡑࡇ࡛ X ⥺ᩓ஘ἲࡢᵓ㐀ゎᯒࢆ⏝࠸࡚ࠊ๣࡟ࡼࡿ⓶⭵ゅᒙࡢศᏊࣞ࣋ࣝࡢᵓ㐀ኚ໬ࡢ᭷↓ࡸᙉᙅࢆᢕᥱࡍࡿࡇ࡜ࡀྍ⬟࡜࡞ࢀࡤࠊゅᒙᵓ㐀࡜ࡑࢀ࡟క࠺ゅᒙᶵ⬟࡬ࡢᙳ㡪ࡢ↓࠸ࡶࡋࡃࡣᑡ࡞࠸๣ࢆぢฟࡍࡇ࡜ࡀ࡛ࡁࡿࠋࡇࢀ࡟ࡼࡾࠊㄡ࡛ࡶᏳᚰࡋ࡚౑⏝ྍ⬟࡞ࠊ⓶⭵࡬ࡢస⏝ࡀࡼࡾ⦆࿴࡞〇ရࡢタィᣦ㔪࡟⧅ࡀࡿ࡜⪃࠼ࡽࢀࡿࠋᚑࡗ࡚ࠊࡇࡢࡼ࠺࡞๣࡟ࡼࡿ⓶⭵స⏝ᶵᵓࡢゅᒙᵓ㐀࡟ᇶ࡙ࡃゎ᫂ࡣ㔜せ࡞◊✲ㄢ㢟࡛࠶ࡿࠋᡃࠎࡣࡇࢀࡲ࡛࡟⏺㠃άᛶ๣Ỉ⁐ᾮฎ⌮࡟క࠺⤒᫬ⓗ࡞ࣄࢺ⓶⭵ゅᒙࡢᵓ㐀ኚ໬࡟ࡘ࠸࡚ࠊ≉࡟ゅᒙ⣽⬊ࢆᵓᡂࡍࡿࢣࣛࢳࣥࢱࣥࣃࢡ㉁࡟ὀ┠ࡋ࡚ᑠゅ࣭ᗈゅ X ⥺ᩓ஘ἲ࡟ࡼࡿゎᯒࢆ⾜ࡗ࡚ࡁࡓ[1–4]ࠋࡇࢀࡲ࡛ࡢ◊✲࡛ࡣ⢊ᮎࡸࢩ࣮ࢺ㸦1 ᯛࡣ⣙ 20 ȝm ཌ㸧≧ࡢゅᒙヨᩱࢆࣛࣥࢲ࣒࡟ワࡵ㎸ࡴࠊࡶࡋࡃࡣࢩ࣮ࢺࢆᩘ༑ᯛศ✚ᒙࡍࡿࡇ࡜࡛ X ⥺ࣅ࣮࣒ᚄ㸦㏻ᖖȭᩘⓒ ȝm㸧௨ୖࡢࢧ࢖
ࢬ࡟ࡋࡓヨᩱ࡟ X ⥺ࣅ࣮࣒ࢆ↷ᑕࡋ࡚ ᐃࡋ࡚࠸ࡓࠋࡍ࡞ࢃࡕࠊゅᒙࡢᵓ㐀᝟ሗࡣ㞟ྜయ࠿ࡽࡢ
ᖹᆒ᝟ሗ࡜ࡋ࡚ᚓ࡚࠸ࡓࠋࡋ࠿ࡋ࡞ࡀࡽࠊゅᒙࡣ 1 ᯛ୰࡛ࡶୖ㒊࠿ࡽୗ㒊࡟Ώࡗ࡚ᵓ㐀ࡀኚ໬ࡋ࡚࠸ࡿࡇ࡜ࡀ㟁Ꮚ㢧ᚤ㙾࡟ࡼࡿほᐹ࠿ࡽ▱ࡽࢀ࡚࠸ࡿ[5]ࠋࡲࡓ⏺㠃άᛶ๣➼ࡀ⓶⭵࡟స⏝ࡋࡓሙ
ྜࡶゅᒙୖ㒊࠿ࡽᾐ㏱ࡋࠊ῝ࡉ᪉ྥ࡟స⏝ࡢ⤖ᯝ࡜ࡋ࡚ᵓ㐀ኚ໬ࡢศᕸࡀ࡛ࡁࡿࡇ࡜ࡀ⪃࠼ࡽࢀࡿࠋᚑࡗ࡚ࠊ๣ࡢస⏝࡟ࡼࡿゅᒙᵓ㐀ࡢኚ໬ࡶ୍ᵝ࡟㉳ࡇࡿࡢ࡛ࡣ࡞ࡃࠊ῝ࡉ᪉ྥ࡟ศᕸࢆᣢࡗ
࡚⏕ࡌࡿ࡜᥎ᐃࡉࢀࡿࠋゅᒙෆ㒊ࡢ῝ࡉ᪉ྥࡢᵓ㐀ኚ໬ࢆゎᯒࡍࡿࡇ࡜࡛ࠊࡉࡽ࡟⓶⭵࡬ࡢ๣ࡢస⏝ࡢヲ⣽࡞▱ぢࡀᚓࡽࢀࡿࡇ࡜ࡀᮇᚅࡉࢀࡿࠋ
ࡑࡇ࡛ᡃࠎࡣ 2015B ᮇࡼࡾ BL40XU ࡢ࣐࢖ࢡࣟࣅ࣮࣒ X ⥺ࢆ฼⏝ࡋࡓゅᒙ 1 ᯛ୰ࡢ῝ࡉ᪉ྥࡢᵓ㐀ゎᯒࡢ᳨ウࢆ㛤ጞࡋࡓࠋᘬࡁ⥆ࡁ BL40XU ࡛ࡢᐇ㦂ࢆ⏦ㄳࡋࡓࡀ᥇ᢥࡉࢀࡎࠊ2016A ➨ 2
ᮇ࡛ BL46XU ࡟࡚ᐇ㦂ࢆ⥅⥆ࡍࡿࡇ࡜࡜࡞ࡗࡓࠋ๓ᅇ࡜ගᏛ⣔➼ࡀ␗࡞ࡿࡓࡵࠊᮏᐇ㦂࡛ࡣヨᩱࡢタ⨨࡜↷ᑕ᮲௳➼ࡢ෌᳨ウࢆ⾜࠸ࠊゅᒙ 1 ᯛ୰ࡢ῝ࡉ᪉ྥࡢᵓ㐀ゎᯒࡢᡭἲࢆ☜❧ࡍࡿࡇ࡜ࢆ┠ᶆ࡜ࡋࡓࠋᐇ㦂㸸1) ゅᒙヨᩱ㸸࣐࢖ࢡࣟࣅ࣮࣒ X ⥺ᩓ஘ἲࢆ⏝࠸ࡓゅᒙ 1 ᯛ୰ࡢᵓ㐀ゎᯒ࡜ࡋ࡚ࡣࠊࣟࣞ࢔ࣝࡢ
Jean Doucet ࡽ࡟ࡼࡿゅᒙࢩ࣮ࢺࢆᚤᑠ࡞▷෉≧࡟ษࡾฟࡋ࡚ほ ࡋࡓሗ࿌[6]ࡀ࠶ࡿࡀࠊࡇࡢ䠉㻞㻡㻣䠉SPring-8/SACLA ฼⏝◊✲ᡂᯝ㞟

SectionSection Bࡼ࠺࡞ᚤᑠษ∦࡛ࡣ๣ᾐ㏱ࡢ᳨ウࡣᅔ㞴࡛࠶ࡾࠊ࠿ࡘ஝⇱ࡢᙳ㡪ࡶᠱᛕࡉࢀࡿࠋࡑࡇ࡛ᡃࠎࡣ
in situ ࡟㏆࠸᮲௳࡛ࡢゅᒙࡢ῝ࡉ᪉ྥࡢᵓ㐀ゎᯒἲ࡜ࡋ࡚ࠊᚑ᮶ࡢከࡃࡢᐇ㦂ࡢࡼ࠺࡟ฎ⌮ࡉ
ࢀࡓゅᒙ㒊ศࡢࡳࡢࢩ࣮ࢺࢆ⏝࠸ࡿࡢ࡛ࡣ࡞ࡃࠊゅᒙ௨ୗࡢ⾲⓶ࠊ┿⓶➼ࡶྵࡴ෾⤖ࣄࢺ⓶⭵ࢩ࣮ࢺ㸦⭡㒊⏤᮶ࠊࢩ࣮ࢺ≧ࠊ1×1 cm2ࠊ⣙ 400 ȝm ཌࠊBIOPREDIC International ♫(ࣇࣛࣥࢫ)ࡼࡾ㉎ධ㸧ࢆ ᐃ๓࡟ゎ෾ࡋヨᩱ࡜ࡋࡓࠋ2) ヨᩱタ⨨ἲ㸸࣐࢖ࢡࣟࣅ࣮࣒ X ⥺ࡀゅᒙ㒊ศࢆ㏻㐣ࡍࡿࡼ࠺࡟ࠊᅗ㸯࡟♧ࡋࡓࡼ࠺࡟࢔࣑ࣝࢸ࣮ࣉࢆᣳࢇ࡛⓶⭵ࢩ࣮ࢺࢆᢡࡾ᭤ࡆࠊ❆ᮦࣇ࢕࣒ࣝ࡜࡜ࡶ࡟ࢫࣛ࢖ࢻ࣐࢘ࣥࢺෆ࡟タ⨨ࡋࡓࠋ3) ౑⏝⿦⨨࣭ ᐃ᮲௳㸸SPring-8 ࡢ BL46XU ࢆ฼⏝ࡋࡓࠋ᳨ฟჾ࡟ࡣࠊPILATUS-2M ࢆ⏝࠸࡚ 2ḟඖᩓ஘ീࢆྲྀᚓࡋࡓࠋX ⥺ࡢ࢚ࢿࣝࢠ࣮㸦Ἴ㛗㸧ࡣ 12.4 keV㸦0.1 nm㸧ࠊ࣐࢖ࢡࣟࣅ࣮࣒ X⥺ࡢࣅ࣮࣒ᚄࡣỈᖹ᪉ྥ 3.12 ȝmࠊ㖄┤᪉ྥ 2.08 ȝm㸦್༙ᖜ㸧ࠊ࣓࢝ࣛ㛗ࡣ 497 mm㸦࣋࣊ࣥ㓟㖟⢊ᮎヨᩱࡢᅇᢡࣉࣟࣇ࢓࢖࡛ࣝ㍑ṇ㸧࡜ࡋࡓࠋ࣐࢖ࢡࣟࣅ࣮࣒ᙧᡂ࡟ࡣࣇࣞࢿࣝࢰ࣮ࣥࣉ࣮ࣞࢺࢆ⏝࠸ࡓࠋ㟢ග᫬㛫ࡣ 1 s㸦↷ᑕᦆയ᳨ウ⏝࡟ࡣ௚ࡢ㟢ග᫬㛫ࡶᐇ᪋㸧࡛ࠊ ᐃࡣᐊ㸦 ㄪ࡞ࡋ㸧࡛ᐇ᪋ࡋࡓࠋゅᒙ᭱⾲㠃࠿ࡽ῝ࡉ᪉ྥ࡟㡰ḟ↷ᑕ఩⨨ࡀኚࢃࡿࡼ࠺࡟ヨᩱࢫࢸ࣮ࢪࢆ⛣ືࡉࡏ࡞ࡀࡽࠊᑠゅ࣭ᗈゅᩓ஘ീࢆྛ↷ᑕ఩⨨࡛ྲྀᚓࡋࡓࠋ4) ࢹ࣮ࢱゎᯒ㸸ᚓࡽࢀࡓ 2 ḟඖᩓ஘ീࢆゅᒙ⾲㠃࡟ᑐࡋ࡚ᆶ┤࣭ᖹ⾜᪉ྥ࡟s10rࡢ⠊ᅖ࡛ᡪᙧᖹᆒࢆィ⟬ࡋ࡚ 1 ḟඖᩓ஘ࣉࣟࣇ࢓࢖ࣝࢹ࣮ࢱࢆᚓࡓࠋ

ᅗ㸯 ヨᩱタ⨨᪉ἲ⤖ᯝ࠾ࡼࡧ⪃ᐹ㸸
 2015B ᮇࡢ BL40XU ࡢᐇ㦂࡜ࡣࣅ࣮࣒ࣛ࢖ࣥࡀ␗࡞ࡿࡓࡵࠊ௒ᅇࡢᐇ㦂࡛ࡣࠊࡲࡎ㧗ᙉᗘࡢ࣐࢖ࢡࣟࣅ࣮࣒ X ⥺࡟ࡼࡿヨᩱࡢ↷ᑕᦆയ࡟ࡘ࠸࡚ࠊ㟢ග᫬㛫ࡢኚ᭦㸦300 ms ࠿ࡽ 10 s ࡲ࡛㸧ࠊྠ୍㒊఩࡛ࡢ⧞㏉ࡋ↷ᑕ࡞࡝࡟ࡼࡿ᳨ウࢆᐇ᪋ࡋࡓࠋࡑࡢ୍౛࡜ࡋ࡚ゅᒙ⾲㠃࠿ࡽ⣙ 10 ȝm ࡢ఩⨨࡟࠾ࡅࡿ㟢ග᫬㛫 1 s ࡛ࡢྠ୍㒊఩⧞㏉ࡋ↷ᑕᐇ㦂ࡢ⤖ᯝࢆᅗ㸰࡟♧ࡍࠋ8 ᅇࡢ⧞㏉ࡋ↷ᑕ࡛ゅᒙࡢ⣽⬊㛫⬡㉁ᵓ㐀ࡀᾘኻࡋ࡚࠸ࡗࡓࡶࡢ࡜⪃࠼ࡽࢀࡓࠋࡇࢀࡽࡢ⤖ᯝࡼࡾࠊ↷ᑕ఩⨨ࢆࡎࡽࡋ࡞ࡀࡽࡢ ᐃ࡛࠶ࢀࡤ㟢ග᫬㛫ࡣ 1 s ࡢሙྜ࡟ヨᩱᦆയ࡜ᩓ஘ࣉࣟࣇ࢓࢖ࣝࡢ SB ẚࡢࣂࣛࣥࢫࡀ㐺ษ࡛࠶ࡿ࡜ุ᩿ࡋࠊ௨㝆ࡢ ᐃࡢ↷ᑕ᫬㛫ࡣ 1 s ࡜ࡋࡓࠋ ḟ࡟ᗄࡘ࠿ࡢ᮲௳࡛ ᐃࢆᐇ᪋ࡋࡓ୰ࡢ௦⾲ⓗ࡞⤖ᯝࢆᅗ㸱࡟♧ࡍࠋᢡࡾ᭤ࡆࡓᮍฎ⌮⓶⭵ࢩ࣮ࢺࡢ⾲㠃ୖ᪉ࡼࡾ 2 ȝm ࢫࢸࢵࣉ࡛࣐࢖ࢡࣟࣅ࣮࣒ X ⥺ࡢ↷ᑕ఩⨨ࢆ⛣ືࡉࡏࡓ࡜ࡁࡢ㸰ḟඖᩓ஘ീ࡜ᆶ┤࠾ࡼࡧỈᖹ᪉ྥࡢᡪᙧᖹᆒᩓ஘ࣉࣟࣇ࢓࢖ࣝࡢ୍㒊ࢆ⾲♧ࡋࡓࡶࡢ࡛࠶ࡿࠋᅗ㸱࡟♧ࡋࡓᩓ஘ࣉࣟࣇ࢓࢖ࣝࡣゅᒙ⠊ᅖෆ࠿ࡽࡢࡶࡢ࡜⪃࠼ࡽࢀࠊ▮༳࡛♧ࡋࡓ఩⨨࡟␗᪉ⓗ࡞ᩓ஘ࣆ࣮ࢡࡀほ ࡉࢀࡓࠋࡇࢀࡽࡢࣆ࣮ࢡࡣࠊࡇࢀࡲ࡛ࡢሗ࿌➼[3,4]ࢆཧ⪃࡟ᆶ┤᪉ྥࡢᩓ஘ࣉࣟࣇ࢓࢖ࣝࡢᑠゅ㡿ᇦ࡟ࡘ࠸࡚ࡣゅᒙࡢࢣࣛࢳࣥ⥺⥔࠾ࡼࡧ⣽⬊㛫⬡㉁࣓ࣛࣛࡢ࿘ᮇ㸦㛫㝸㸧⏤᮶ࡢᩓ஘ࣆ࣮ࢡࠊỈᖹ᪉ྥࡢᗈゅ㡿ᇦ࡟ࡘ࠸࡚ࡣ⣽⬊㛫⬡㉁ࡢ࢔ࣝ࢟ࣝ㙐ࣃࢵ࢟ࣥࢢ⏤᮶ࡢᩓ஘ࣆ࣮ࢡ࡟ᖐᒓࡍࡿ࡜⪃࠼ࡽࢀࡓࠋࡲࡓࠊࡇࢀࡽࡢᩓ஘ࣉࣟࣇ࢓࢖ࣝࡣゅᒙ⾲㠃࠿ࡽࡢ῝ࡉ࡟౫Ꮡࡋ࡚ኚ໬ࡋ࡚࠸ࡿᵝᏊࡀほࡽࢀࡓࠋ䠉㻞㻡㻤䠉SPring-8/SACLA ฼⏝◊✲ᡂᯝ㞟SectionSection Bᅗ㸰 ྠ୍㒊఩⧞㏉ࡋ↷ᑕᐇ㦂࡛ࡢ 2 ḟඖᩓ஘ീ㸦୰ᚰ௜㏆㸧࡜ᆶ┤᪉ྥࡢᡪᙧᖹᆒᩓ஘ࣉࣟࣇ
࢓࢖ࣝኚ໬ࠋ2 ḟඖᩓ஘ീࡢᆶ┤᪉ྥ࡟ぢ࠼ࡿࣆ࣮ࢡࡣࠊq = 1 ࠾ࡼࡧ 1.3 nmí1 ࡟ぢ࠼ࡿࣆ࣮ࢡࠋᩓ஘ീ୰ࡢ㯮⥺㒊ศࡣ PILATUS-2M ࡢ᳨ฟჾ⣲Ꮚࡢ⧅ࡂ┠㸦୙ឤࢠࣕࢵࣉ఩⨨㸧ࠋᅗ㸱 ᮍฎ⌮ゅᒙ࠿ࡽࡢ㸰ḟඖᩓ஘ീ࡜ᆶ┤࠾ࡼࡧᖹ⾜᪉ྥࡢᡪᙧᖹᆒᩓ஘ࣉࣟࣇ࢓࢖ࣝࠋᩘᏐࡣゅᒙ⾲㠃࠿ࡽࡢ῝ࡉ᪉ྥࡢ఩⨨ࠋ▮༳ࡣゅᒙ୰ࡢ⣽⬊㛫⬡㉁ᵓ㐀⏤᮶࡜᥎ᐃࡉࢀࡿࣆ࣮ࢡࠋྛᩓ஘ࣉࣟࣇ࢓࢖ࣝࡣ㔜࡞ࡽ࡞࠸ࡼ࠺࡟⦪㍈᪉ྥࡢ⾲♧఩⨨ࢆࡎࡽࡋ࡚♧ࡋ࡚࠸ࡿࠋࡉࡽ࡟ࠊᅗ㸲࡟♧ࡋࡓࣜࣔࢿࣥฎ⌮ࡢ⓶⭵ࢩ࣮ࢺ࡛ࡢ ᐃ࡛ࡣᮍฎ⌮⓶⭵ࢩ࣮ࢺ࡟ẚ࡭࡚ୖ㏙ࡢࣆ࣮ࢡࡀࣈ࣮ࣟࢻ໬ࡋ࡚࠸ࡿᵝᏊࡀほࡽࢀࠊࡲࡓ⾲㠃࡟㏆࠸࡯࡝ᙳ㡪ࡀ኱ࡁ࠸ᵝᏊࡶぢࡽࢀࡓࠋ௒ᅇࡢヨᩱࡢಖᣢ᪉ἲ࡛ࡣࠊゅᒙୗ㒊ࡢ ᐃ࡟ゅᒙୖ㒊ࡢ᝟ሗࡶຍ⟬ࡉࢀࡿࡓࡵࠊཝᐦ࡞῝ࡉ᪉䠉㻞㻡㻥䠉SPring-8/SACLA ฼⏝◊✲ᡂᯝ㞟Section
Section Bྥࡢᵓ㐀ኚ໬ࡢホ౯࡟ࡘ࠸࡚ࡣ᳨ドࡀᚲせ࡛࠶ࡿࠋ୍᪉࡛ࠊᮍฎ⌮ࡢゅᒙࡢࢹ࣮ࢱ࡜ࡢẚ㍑࡟ࡼ
ࡾ┦ᑐⓗ࡟῝ࡉ᪉ྥࡢኚ໬ࡢ⛬ᗘࢆホ౯ࡍࡿࡇ࡜ࡣྍ⬟࡛࠶ࡿ࡜⪃࠼ࡽࢀࠊ௒ᅇࡢᐇ㦂࡛ᐇ᪋ࡋࡓᡭἲ࡟ࡼࡾゅᒙෆ㒊ࡢ῝ࡉ᪉ྥࡢᵓ㐀ኚ໬ࢆゎᯒࡍࡿࡇ࡜ࡢྍ⬟ᛶࡀ♧ࡉࢀࡓࠋᅗ㸲 ࣜࣔࢿࣥฎ⌮ゅᒙ࠿ࡽࡢᆶ┤࠾ࡼࡧᖹ⾜᪉ྥࡢᡪᙧᖹᆒᩓ஘ࣉࣟࣇ࢓࢖ࣝࠋᩘᏐࡣゅᒙ⾲㠃࠿ࡽࡢ῝ࡉ᪉ྥࡢ఩⨨ࠋㄢ㢟࡜௒ᚋࡢணᐃ㸸 ௒ᅇࡢヨᩱࡢಖᣢ᪉ἲ࡛ࡣࠊゅᒙୗ㒊ࡢ ᐃ࡟࡚ゅᒙୖ㒊ࡢ᝟ሗࡶຍ⟬ࡉࢀࡿࠋ୍᪉࡛ࠊゅᒙෆࡢࢣࣛࢳࣥ⥺⥔࠾ࡼࡧ⣽⬊㛫⬡㉁࣓ࣛࣛᵓ㐀ࡣᡥᖹ࡞ゅᒙ⣽⬊࡟ἢࡗ࡚⾲㠃࡜Ỉᖹ࡟㓄ྥࡋ࡚࠾ࡾ[3,4]ࠊゅᒙ࡟ᑐࡋ edge ᪉ྥ࡟ X ⥺ࢆධᑕࡍࡿ࡜ᵓ㐀⏤᮶ࡢᩓ஘ࡀ㢧ⴭ࡟ᚓࡽࢀࡿࡀ through᪉ྥ࡟ධᑕࡋࡓሙྜ࡟ࡣᩓ஘ࡀᙅࡃ࡞ࡿࠋࡑࡢࡓࡵࠊゅᒙୗ㒊ࡢ ᐃ࡛ࡣ through ධᑕ࡜࡞ࡿୖ㒊ࡢ᝟ሗࡢᙳ㡪ࡣ┦ᑐⓗ࡟ᑡ࡞ࡃ࡞ࡿ࡜⪃࠼ࡽࢀࡿࠋ௒ᚋࡣࠊᐇ㦂ࡸィ⟬ࡢᡭἲ࡟ࡼࡾ X ⥺ࡢ㏱㐣⤒㊰ࢆぢ✚ࡶࡾࠊゅᒙୗ㒊ࡢ ᐃ࡟࡚ゅᒙୖ㒊ࡢᙳ㡪ࢆ᳨ウࡋࡓ࠸ࠋࡇࢀ࡟ࡼࡾ࣐࢖ࢡࣟࣅ࣮
࣒ X ⥺ࢆ฼⏝ࡋࡓゅᒙ୰ࡢ῝ࡉ᪉ྥࡢᵓ㐀ゎᯒᡭἲࢆ☜❧ࡋࠊᵝࠎ࡞๣࡟ࡼࡿᾐ㏱ᐇ㦂ࢆᐇ᪋ࡋ࡚⓶⭵ゅᒙ࡬ࡢ๣ࡢస⏝ᶵᵓࡢゎ᫂࡟⧅ࡆࡿࠋཧ⪃ᩥ⊩㸸
[1] ᒣ⏣┿∞ ௚㸪SPring-8 ฼⏝◊✲ᡂᯝ㞟, 1 (1), B (2012), 2011B1754.
[2] ஂ⡿༟ᚿ ௚㸪᪥ᮏ୰ᛶᏊ⛉Ꮫ఍ㄅ Ἴ⣠, 24 (1), 15 (2014).
[3] ஂ⡿༟ᚿ ௚㸪SPring-8 ฼⏝◊✲ᡂᯝ㞟, 4 (1), B (2016), 2014A1516.
[4] ஂ⡿༟ᚿ ௚㸪SPring-8 ฼⏝◊✲ᡂᯝ㞟, 4 (1), B (2016), 2014B1617.
[5] T. Sano et al., Archives of Dermatological Research 296, 359 (2005)[6] J. Doucet et al., Journal of Lipid Research 55, 2380 (2014).

(Received: November 18, 2016; Early edition: April 10, 2019;
Accepted: July 16, 2019; Published: August 29, 2019)䠉㻞㻢㻜䠉