SPring-8/SACLA ฼⏝◊✲ᡂᯝ㞟

Section B%                              %/%

⏺㠃άᛶ๣ࡢࣄࢺ⓶⭵ゅᒙᵓ㐀࡬ࡢᙳ㡪ゎᯒ࡟ᇶ࡙ࡃ⓶⭵Ὑί⏝໬⢝ရ㛤Ⓨ
Development of Cosmetic Cleanser Based Upon Analysis of Structural
Alternation of Human Stratum Corneum by Surfactant Applications⡀℩ 㤶⧊ a, ඵ⏣ ୍㑻 b Kaori Yanasea, Ichiro Hattabaࢡࣛࢩ࢚࣮࣒࣍ࣉࣟࢲࢡࢶ㸦ᰴ㸧, b㸦බ㈈㸧ྡྂᒇ⏘ᴗ⛉Ꮫ◊✲ᡤ aKracie Home Products, Ltd., bNagoya Industrial Science Research Institute ⓶⭵ࢆΎ₩࡛೺ࡸ࠿࡟ಖࡘࡓࡵ࡟ࠊ࣎ࢹ࢕ࢯ࣮ࣉ࡞࡝ࡢ⓶⭵Ὑίᩱࡀẖ᪥౑⏝ࡉࢀ࡚࠸ࡿࠋ࢔ࢽ࢜ࣥᛶ⏺㠃άᛶ๣ࡣ⓶⭵Ὑίᩱࡢ୺せ࡞ᡂศ࡛࠶ࡾࠊởࢀࢆຠᯝⓗ࡟ⴠ࡜ࡍ୍᪉࡛ࠊゅᒙᵓ㐀࡟ᙳ㡪ࢆཬࡰࡍࠋࡑࡇ࡛ᑠゅᗈゅ X ⥺ᩓ஘ࢆ ᐃࡋゅᒙᵓ㐀ࡢᅇ᚟ࢆ᳨ウࡋࡓࠋࡑࡢ⤖ᯝࠊ⏺㠃άᛶ๣ࢆ⢭〇Ỉ࡟⨨᥮ࡋࡓ᫬ࡢᵓ㐀ᅇ᚟ࡣࠊ௒ᅇ⏝࠸ࡓゅᒙ࡛ࡣ☜ㄆ࡛ࡁ࡞࠿ࡗࡓࠋ࣮࣮࢟࣡ࢻ㸸 ⓶⭵ࠊゅᒙࠊ⣽⬊㛫⬡㉁ࠊ⓶⭵ὙίᩱࠊSodium Dodecyl Sulfate⫼ᬒ࡜◊✲┠ⓗ㸸 ࣎ࢹ࢕ࢯ࣮ࣉࡸὙ㢦ࣇ࢛࣮࣒ࡣ⓶⭵ࢆΎί࡟ࡍࡿࡓࡵẖ᪥౑⏝ࡉࢀࡿࠋ࢔ࢽ࢜ࣥᛶ⏺㠃άᛶ๣ࡣࡑࡢࡼ࠺࡞⓶⭵Ὑί〇ရࡢ୺せᡂศࡔࡀࠊởࢀࢆⴠ࡜ࡍ୍᪉࡛⓶⭵࡟ࢲ࣓࣮ࢪࢆ୚࠼ࡿࡇ࡜ࡀ⮫ᗋⓗ࡟ᣦ᦬ࡉࢀ࡚࠸ࡿࠋゅᒙࡣ⓶⭵ࡢ᭱እᒙ࡛ゅᒙ⣽⬊࡜ゅᒙ⣽⬊㛫⬡㉁࠿ࡽᵓᡂࡉࢀࠊ⬡㉁ࡢ୺࡞ᡂศࡣࢭ࣑ࣛࢻࠊࢥࣞࢫࢸ࣮ࣟࣝࠊ⬡⫫㓟ࡀ࠶ࡿࠋࡇࢀࡽࡢ⬡㉁ࡣゅᒙ࡛࣓ࣛࣛᵓ㐀ࢆ⤌⧊ࡍࡿࠋࡼࡃࡲ࡜ࡲࡗࡓ࣓ࣛࣛᵓ㐀ࡣࠊ⓶⭵ࣂࣜ࢔ᶵ⬟࡟࡜ࡗ࡚㠀ᖖ࡟㔜せࡔࡀࠊὙί࡟ࡼࡾởࢀࡔࡅ࡛࡞ࡃゅᒙᵓ㐀࡟㔜せ࡞⓶⬡ࡶὙ࠸ⴠ࡜ࡍࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿࠋᚑࡗ࡚Ὑί๣ࡣ⓶⭵ࣂࣜ࢔ᶵ⬟ࢆቯࡍ࠿ࡶࡋࢀ࡞࠸ࠋࡼࡗ࡚⏺㠃άᛶ๣ࡀ࡝ࡢࡼ࠺࡟⓶⭵࡟ᙳ㡪ࡍࡿ࠿✲᫂ࡍࡿࡇ࡜ࡣ㔜せ࡛࠶ࡿࠋ ࡇࢀࡲ࡛ࡢ᳨ウ࡛ࣄࢺゅᒙ࡟ Sodium Dodecyl Sulfate (SDS)⁐ᾮࢆῧຍࡋࡓሙྜ࡟ࠊゅᒙᵓ㐀࡟஘ࢀࡀ⏕ࡌࡿࡇ࡜ࡀศ࠿ࡗ࡚࠸ࡿࠋ௒ᅇࡢ᳨ウ࡛ࡣࠊSDS ࡟ࡼࡾ஘ࢀࡓゅᒙᵓ㐀࡟ࡘ࠸࡚ࠊSDS ⁐ᾮࢆỈ࡟⨨᥮ࡍࡿࡇ࡜࡟ࡼࡗ୍࡚ᗘ஘ࢀࡓゅᒙᵓ㐀ࡀᅇ᚟ࡍࡿ࠿࡝࠺࠿᳨ウࡍࡿࠋᐇ㦂㸸 ゅᒙヨᩱࡢ‽ഛ࡜ࡋ࡚ࡣࠊࢺࣜࣉࢩࣥฎ⌮࡟ࡼࡾ⓶⭵⤌⧊ࡼࡾ๤㞳ࡋࡓゅᒙ㸦Biopredic Co., Ltd,France㸧ࢆὙί࣭஝⇱ᚋࠊணࡵ25 wt%ࡢỈศ㔞࡟ㄪ⠇ࡋࡓࠋ ᐃ࡟⏝࠸ࡿ⏺㠃άᛶ๣ࡣࠊࡇࢀࡲ࡛ࡢ ᐃ⤖ᯝ࠿ࡽẚ㍑ⓗᵓ㐀ኚ໬ࡀᙉࡃ⌧ࢀࡓSDSࢆ⏝࠸ࠊ⃰ᗘࢆ10 wt%ࠊpH 7࡟ㄪ〇ࡋࡓࠋ ᐇ㦂ࡣBL19B2࡟࡚ᐇ᪋ࡋࡓࠋ ᐃࡍࡿTࣞࣥࢪࡣゅᒙ⣽⬊㛫⬡㉁ࡢ࣓ࣛࣛᵓ㐀ࡢ࿘ᮇ(G = 13.6nmࠊG = 6 nm௜㏆)ࡔࡅ࡛࡞ࡃࠊⅣ໬Ỉ⣲㙐ࡢ඘ሸᵓ㐀(G = 0.41 nmࠊG = 0.37 nm௜㏆)ࠊゅᒙ⣽⬊ࢆᵓᡂࡍࡿࢣࣛࢳࣥࡢศᏊ㓄ิᵓ㐀(G = 1 nm௜㏆)࡟㉳ᅉࡍࡿᅇᢡࣆ࣮ࢡࢆ࢝ࣂ࣮ࡍࡿࡓࡵࠊ6 =0.07–2.86 nmí1ࡢ㡿ᇦ࡜ࡋࡓࠋࡑࡢࡓࡵࠊX⥺ࡢ࢚ࢿࣝࢠ࣮ࡣ28 keVࠊ࣓࢝ࣛ㛗ࡣ700 nm㸦࣋࣊ࣥ㓟㖟ࡢᅇᢡࣆ࣮ࢡࢆ⏝࠸࡚㍑ṇ㸧࡜ࡋࡓࠋ᳨ฟჾ࡟ࡣPILATUS 2Mࢆ⏝࠸ࡓࠋࡲࡓ⏺㠃άᛶ๣ࡢᙳ
㡪ࢆホ౯ࡍࡿࡓࡵࠊẚ㍑࡜ࡋ࡚Ỉࡢࡳࢆὀධࡋࡓヨᩱ࡟ࡘ࠸࡚ࡶホ౯ࡋࡓࠋゅᒙヨᩱࢆ⁐ᾮࢭࣝ
㹙1㹛࡟඘ሸࡋ࡚ヨᩱࢫࢸ࣮ࢪ࡟ࢭࢵࢺࡋࠊࢭࣝෆ࡟⏺㠃άᛶ๣⁐ᾮࢆὀධࡋ࡚ࡑࡢX⥺ᅇᢡࣉࣟࣇ࢓࢖ࣝࡢ᫬㛫ኚ໬ࢆ ᐃࡋࡓࠋ1ᅇ࡟ࡘࡁ30⛊ࡢ㟢ග᫬㛫ࢆ3ศࡲࡓࡣ5ศẖ࡜ࡋ࡚ࠊ⏺㠃άᛶ๣⁐ᾮ㐺⏝ᚋ6᫬㛫ࡲ࡛᫬㛫⤒㐣ࢆ㏣࠸ࠊ୍᪦೵Ṇࡋ⁐ᾮࢆ⢭〇Ỉ࡟඘ศ⨨᥮ᚋ࡟ࠊྠࡌࡼ࠺࡟1ᅇࡢ㟢ග᫬㛫30⛊ࢆ3ศẖ࡟2᫬㛫ᚋࡲ࡛᫬㛫⤒㐣ࢆ㏣࠸ࠊゅᒙࡢᵓ㐀ኚ໬ࢆほᐹࡋࡓࠋ

䠉㻞㻝㻢䠉SPring-8/SACLA ฼⏝◊✲ᡂᯝ㞟Section B⤖ᯝ࠾ࡼࡧ⪃ᐹ㸸 ࣄࢺゅᒙ࡟SDSࢆῧຍࡋࡓ᫬㛫⤒㐣ࣉࣟࣇ࢓࢖ࣝ࠾ࡼࡧࠊSDSฎ⌮ᚋ⁐ᾮࢆ⢭〇Ỉ࡟஺᥮ࡋࠊ⤒㐣ࢆ2᫬㛫㏣㊧ࡋࡓࣉࣟࣇ࢓࢖ࣝࢆᅗ㸯ࠊ㸰࡟♧ࡍࠋᅗ୰ࡢ◚⥺ࡣSDSῧຍ๓ࡢゅᒙࣉࣟࣇ࢓࢖ࣝ
ࢆ♧ࡋࠊSDS࠿ࡽ⢭〇Ỉ࡬⨨᥮ᚋࡢࣉࣟࣇ࢓࢖ࣝ࡟ࡘ࠸࡚ୗ࠿ࡽୖ࡬᫬㛫⤒㐣ࢆ♧ࡍࠋ ᅗ㸯୰࡛ࡣ6= 0.16 nmí1௜㏆࡟▷࿘ᮇ࣓ࣛࣛᵓ㐀ࡢ࿘ᮇ࡟⏤᮶ࡍࡿ1ḟ཯ᑕࣆ࣮ࢡࡀ☜ㄆࡉࢀࠊ
SDSῧຍ࡟ࡼࡾࢃࡎ࠿࡟పゅഃ࡬ࢩࣇࢺࡋࡓࠋ⢭〇Ỉ࡟஺᥮ࡋ࡚࠿ࡽࡣࠊࣆ࣮ࢡ఩⨨ኚ໬ࡣ࡯࡜ࢇ࡝ぢࡽࢀ࡞࠿ࡗࡓࡀࠊ15ศ⤒㐣ᚋ࠿ࡽࣆ࣮ࢡࡀ㸰ࡘ࡟ศࢀࡓࠋ▷࿘ᮇ࣓ࣛࣛᵓ㐀࡛ࡣࠊゅᒙ඲యࡢỈศ㔞ࡢቑຍ࡜ඹ࡟ࠊ⬡㉁ࡢỈᒙࡢཌࡉࡀቑຍࡍࡿࡇ࡜ࡀሗ࿌ࡉࢀ࡚࠸ࡿࡀ㹙2㹛ࠊࣆ࣮ࢡࡢ
ศ㞳ࡣSDS⁐ᾮࢆ㐺⏝ࡋࡓ࡜ࡁ≉᭷ࡢኚ໬࡛࠶ࡿࡓࡵࠊ⁐ᾮࢆ⢭〇Ỉ࡟஺᥮ᚋࡶ⬡㉁ᒙ㛫࡟SDSࡀ౵ධࡋࡓࡇ࡜࡛࿘ᮇᵓ㐀࡟ᙳ㡪ࢆཬࡰࡋ࡚࠸ࡿࡇ࡜ࢆ♧၀ࡍࡿࡶࡢ࡜⪃࠼ࡽࢀࡿࠋ୍᪉ࠊᅗ㸰࡛ࡣ6 = 2.4 nmí1௜㏆࡜6 = 2.7 nmí1௜㏆࡟⣽⬊㛫⬡㉁඘ሸᵓ㐀⏤᮶ࡢࣆ࣮ࢡࡀ☜ㄆࡉࢀࡓࡀࠊSDSῧຍཬࡧ⢭〇Ỉ࡬ࡢ⨨᥮࡛࡯࡜ࢇ࡝ኚ໬ࡀࡳࡽࢀ࡞࠿ࡗࡓࠋࡇࢀࡲ࡛ࡢ᳨ウ࡛ࠊSDSࢆゅᒙ࡟㐺⏝ࡍࡿ࡜ࠊゅᒙ⣽⬊㛫⬡㉁ࡢ࣓ࣛࣛᵓ㐀ࡣࠊ㛗࿘ᮇ࣓ࣛࣛᵓ㐀ࡀ⭾₶ࡋࠊ᫬㛫ࡀ⤒ࡘ࡜㛗࿘ᮇ࣓ࣛࣛᵓ㐀ࡀᦆയࡋࠊࡲࡓ▷࿘ᮇ࣓ࣛࣛᵓ㐀ཬࡧഃ᪉඘ሸ㓄ิᵓ㐀ࡣࠊ࡯࡜ࢇ࡝ኚ໬ࢆ♧ࡉ࡞࠸ࡇ࡜ࢆ☜ㄆࡋ࡚࠸ࡿࠋ࣓ࣛࣛᵓ㐀ࡣ QP ࡢ㛗࿘ᮇᵓ㐀ࢆࡶࡘࡶࡢ࡜ࠊ QP ⛬ᗘࡢ▷࿘ᮇᵓ㐀ࢆᣢࡘࡶࡢࡀᏑᅾࡍࡿࡇ࡜ࢆ᫂ࡽ࠿࡟࡞ࡗ࡚࠸ࡿࡀ[3]ࠊ௒ᅇ⏝࠸ࡓࣄࢺゅᒙ࡛ࡣࠊSDSῧຍ๓࡟࠾࠸࡚ࡶࠊ㛗࿘ᮇ࣓ࣛࣛᵓ㐀࡟⏤᮶ࡍࡿࣆ࣮ࢡࡀ᫂☜࡟☜ㄆࡉࢀ࡞࠿ࡗࡓࡓࡵࠊSDS࡟ࡼࡿᙳ㡪ࢆほᐹࡍࡿࡇ࡜ࡀฟ᮶࡞࠿ࡗࡓࠋᅗ㸯㸬 ࣄࢺゅᒙࡢᑠゅX⥺ᅇᢡࣉࣟࣇ࢓࢖ࣝ◚⥺ࡣ⁐ᾮฎ⌮๓ࡢゅᒙࣉࣟࣇ࢓࢖ࣝࢆ♧ࡍ(A) SDSฎ⌮ᚋࡢ᫬㛫⤒㐣ࣉࣟࣇ࢓࢖ࣝSDSῧຍᚋࡢ᫬㛫⤒㐣ࡣࠊୗ࠿ࡽୖ࡟ྥ࠿ࡗ࡚㸱ศẖ࡟120ศࡲ࡛ࢆ♧ࡍࠋ(B) ⢭〇Ỉ஺᥮ᚋࡢ᫬㛫⤒㐣ࣉࣟࣇ࢓࢖ࣝ⢭〇Ỉ஺᥮ᚋࡢ⤒㐣᫬㛫ࡣࠊୗ࠿ࡽୖ࡟ྥ࠿ࡗ࡚㸱ศẖ࡟120ศࡲ࡛♧ࡍࠋ(B-1) ࣆ࣮ࢡࡢ඾ᆺ౛㸪3ศᚋ (B-2)ࣆ࣮ࢡࡢ඾ᆺ౛㸪111ศᚋ

䠉㻞㻝㻣䠉SPring-8/SACLA ฼⏝◊✲ᡂᯝ㞟

Section Bᅗ㸰㸬 ࣄࢺゅᒙࡢᗈゅX⥺ࣉࣟࣇ࢓࢖ࣝ◚⥺ࡣ⁐ᾮฎ⌮๓ࡢゅᒙࣉࣟࣇ࢓࢖ࣝࢆ♧ࡍࠋ(A) SDSฎ⌮ᚋࡢ᫬㛫⤒㐣ࣉࣟࣇ࢓࢖ࣝSDSῧຍᚋࡢ᫬㛫⤒㐣ࡣࠊୗ࠿ࡽୖ࡟ྥ࠿ࡗ࡚㸱ศẖ࡟120ศࡲ࡛ࢆ♧ࡍࠋ(B) ⢭〇Ỉ஺᥮ᚋࡢ᫬㛫⤒㐣ࣉࣟࣇ࢓࢖ࣝ⢭〇Ỉ஺᥮ᚋࡢ⤒㐣᫬㛫ࡣࠊୗ࠿ࡽୖ࡟ྥ࠿ࡗ࡚㸱ศẖ࡟120ศࡲ࡛♧ࡍࠋ௒ᚋࡢㄢ㢟㸸 ௒ᅇ⏝࠸ࡓࣄࢺゅᒙヨᩱࡣ㛗࿘ᮇ࣓ࣛࣛᵓ㐀࡟⏤᮶ࡍࡿࣆ࣮ࢡࡀ࡯࡜ࢇ࡝ほᐹࡉࢀ࡞࠿ࡗࡓࠋ⏕యヨᩱࡣಶయᕪࡀ኱ࡁ࠸ࡇ࡜ࡣࡸࡴࢆᚓ࡞࠸ࡀࠊࡑࡢࡓࡵ㛗࿘ᮇ࣓ࣛࣛᵓ㐀࡟㛵ࡋ࡚▱ぢࡀᚓࡽࢀ࡞࠿ࡗࡓࠋ௒ᚋูࡢヨᩱࢆ⏝࠸࡚෌ᗘヨ㦂ࢆᐇ᪋ࡍࡿணᐃ࡛࠶ࡿࠋ ࡲࡓࠊ௒ᅇ⏺㠃άᛶ๣⁐ᾮ࠿ࡽ⢭〇Ỉ࡬ࡢ⨨᥮࡟ࡘ࠸࡚ࠊ ᐃ୰࡟⢭〇Ỉࢆࢭࣝ୰࡟ࡺࡗࡃࡾᚠ⎔ࡉࡏ⥆ࡅ⏺㠃άᛶ๣ࡢΰᅾࢆ㜵ࡄࡼ࠺ヨࡳࡓࡀࠊ࣏ࣥࣉ⬟ຊࡀ୙㊊ࡋᚠ⎔࡛ࡁࡎࠊ ᐃ㛤ጞ᫬࡟ᡭື࡛஺᥮ࡋࡓࡓࡵࠊ஺᥮୰ࡢ ᐃࡀ࡛ࡁࡎ᫬㛫⤒㐣ࢆ⪃ᐹࡍࡿ࡟ࡣ୙༑ศ࡛࠶ࡗࡓࠋᚠ⎔࣏ࣥࣉࡢタ⨨࡟᝿ᐃ௨ୖ࡟᫬㛫ࢆせࡋࡓࡓࡵࠊᙜึணᐃࡋ࡚࠸ࡓࣛ࢘ࣜࣥ㓟࣒࢝ࣜ࢘ࡢ ᐃࡣ୰Ṇࡋࡓࠋཧ⪃ᩥ⊩㸸
[1] I. Hatta, et al., &KHP3K\V/LSLGV, 163, 381 (2010).
[2] N. Ohta, et al., &KHP3K\V/LSLGV, 123, 1 (2003).
[3] J. A. Bouwstra, et al., -,QYHVW'HUPDWRO,97, 1005 (1991).

(Received: March 27, 2015; Early edition: May 9, 2019,
Accepted: July 16, 2019; Published: August 29, 2019䠉㻞㻝㻤䠉