Agilent Cary Eclipse
Fluorescence
Spectrophotometer
Applications

The benefits of xenon

Applications for Life Sciences

With unique, xenon flash lamp
technology the Cary Eclipse is capable offluorescence, phosphorescence, chemiand bioluminescence measurements.

Characterize bio-labels for live cell imaging

Detecting specific bacterial strains using fluorescent assay
Measuring probe fluorescence to detect specific bacterial strains — get thebenefits of room light immunity while adding reaction components, andno warm up time! (Aguirre et al. 2012 J Vis Exp Vol. 63 doi: 10.3791/3961).
See Figure 2.
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900
7500.04

600

0.03

4500.02

3000.01

15000400

450500550600

Wavelength (nm)Figure 1. Absorbance/excitation (blue) andfluorescence (red) spectra of fluoresceindye. Fluorescein derivatives are used for
fluorescence assays such as ELISA andfluorescence microscopy.

Fluorescence Intensity (a.u.)

The Cary Eclipse has the lowest costof ownership as the lamp typically lasts
over 10 years. Save money over the lifeof the instrument.

Rapid kinetics showing G-Protein coupled receptor oligomerization. With theCary Eclipse measure time based experiments as well as analyze emission
spectra (Pellissier et al. 2011 J Biol Chem Vol 286(12) doi:10.1074/jbc.
M110.201939).

100
Intensity (a.u.)Work with the sample compartment open!
With exceptionally fast data collection,a scan of the entire wavelength range
can be completed in under three seconds.
Or collect 80 points per second forfast kinetics measurements at a single
wavelength.

Characterizing GPCR oligomerization

Absorbance

The Cary Eclipse optical design enables
measurements of samples sensitive
to light without photo-bleaching, yetalso provides room light immunity for
fluorescence measurements.

Measure luminescence and quantum yield of nanocrystals (quantum dots)
using the Cary Eclipse and a standard quartz cuvette (Byrne et al. 2005 Procof SPIE Vol. 5824 doi:10.1117/12.604814). See Figure 1.

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540

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Wavelength (nm)Figure 2. Room light immunity –sample
compartment closed (blue trace) and open(red trace).

Understanding platelet response using cellular signaling

1.05

Ratio 340/380

Cary WinFLR software has a dedicated ‘Ratio’ application ... allowing
measurement of intracellular ions, such as calcium ion mobilization, that may
occur in specific cell types. With the fast filter accessory, data can be collected
every 50 ms (Petrucci et al. 2011 J. Pharm and Exp Ther Vol. 336 doi:10.1124/
jpet.110.174821). See Figure 3.

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0.95
0.90
0.85

Analyze changes in tertiary structure of proteins
Analysis of lyophilized proteins (solid state) using the Cary Eclipse anda powder cell holder — another tool for the analysis of the stability ofpharmaceuticals during long term storage (Ramachandar et al. 2008
Analytical Biochem Vol. 376 doi:10.1016/j.ab.2008.02.008). See Figure 4.

0.5

With a range of easy-to-use accessories available, the Cary Eclipse is able todo any fluorescence application.
380861
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360

730

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664

Wavelength (nm)

Inyensity (a.u.)

340
600

400

599

330

533

320

467

310

402

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450

500

550

Wavelength (nm)600

Intensity (a.u.)

123205

270

73

260

85Figure 4. Analyze fluorescence lifetimes, such as
those of lanthanide chelates used for fluorescentbioassays.
10080
Derivative

60
40Tm
20139

4Time (ms)

270

280

0100

203363002902002000Intensity (a.u.)

Application flexibility

800

2.0300

The Cary Eclipse with temperature control using the four cell peltier accessory
is capable of ramping sample temperature for analysis of thermal stability.
Ideal for applications such as analyzing the influence of protein quarternary
structure on stability and looking at aggregation and long term storage ofpharmaceuticals (van Lieshout et al. 2012 Appl Biochem Biotechnol Vol. 167
doi: 10.1007/s12010-012-9674-z). See Figure 5.

370

1.5Figure 3. Intracellular Ca2+ concentration
determined with 340/380 ratio.

Thermal stability of biocatalysts and pharmaceuticals

1000

1.0Time (min)406080100

Temperature (°C)

400 425 450 475 500 525 550 575 600

Wavelength (nm)3D and contour software
viewing

Fiber optics for liquid andsolid samples

Temperature control withsample probes

Microplate reader

For more information visit the Agilent
website at:www.agilent.com/chem/cary-eclipse
This information is subject to change without notice.
© Agilent Technologies, Inc. 2019
Published in the USA, April 19, 20195991-3842EN

Automatic polarizers/fastfilters

Figure 5. Tm value using 13mer DABCYL labelled
PNA probe with 5’ 6-carboxyfluorescein labelled
DNA. Tm was calculated by performing a derivative
curve using the Cary Eclipse thermal software.
It was calculated to be 63.9 °C.