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expression of cyclooxygenase-2 in the spinal cordof rats with Freund's adjuvant-induced chronic
inflammatory pain. Previous studies demonstrated
the up-regulation of cyclooxygenase-2 (COX-2)

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expression in the spinal cord of rats withneuropathic pain. The present study was
undertaken to determine whether there was asimilar up-regulation of COX-2 in rats with
inflammatory pain. Intraperitoneal injection of
Freund's complete adjuvant into the hind paw ofrats on days 1 and 5 resulted in substantial
mechanical hyperalgesia, accompanied byswelling of the injected paw and minimal thermal
hyperalgesia. It was demonstrated that there wasan increase in COX-2 immunoreactivity, mainlyin the superficial and deep laminae of theipsilateral spinal cord, in contrast to the minimal
immunoreactivity observed in the corresponding
region of vehicle-treated animals. This increase in
COX-2 immunoreactivity was accompanied by amarked increase in COX-2 mRNA expression.
The COX-2 immunoreactivity was localized inboth spinal cord cells and fibers. In cells, theimmunoreactivity was clearly observed in glialcells, not in neurons. In the spinal cord fibers,

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COX-2 immunoreactivity was markedly
increased in the ipsilateral side of the spinal cord.
This up-regulation of COX-2 expression in thespinal cord is associated with the development ofinflammatory pain.1 f678ea9f9e
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Rurouni Kenshin 1080p Yify Torrent