SPring-8/SACLA ฼⏝◊✲ᡂᯝ㞟
2015A1990

Section B

BL19B2㉸ᑠゅ X ⥺ᩓ஘࡟ࡼࡾゎᯒࡋࡓᑠ㯏ࢱࣥࣃࢡ㉁Ỉ࿴จ㞟యࡢ㞟ྜᵓ㐀Structural Analysis on Hydrated Wheat Protein Assembly byUltra-Small-Angle X-ray Scatteringబ⸨ ಙᾈ a, ⿬ฟ ௧Ꮚ a, b, ᮡᒣ ṇ᫂ aNobuhiro Sato a, Reiko Urade a, b, Masaaki Sugiyama aி㒔኱Ꮫ a 」ྜཎᏊຊ⛉Ꮫ◊✲ᡤ, b ኱Ꮫ㝔㎰Ꮫ◊✲⛉
Kyoto University, a Institute for Integrated Radiation and Nuclear Science, b Graduate School of Agriculture⣧Ỉ୰࡟ᢳฟࡉࢀࡓᑠ㯏ࢱࣥࣃࢡ㉁ࢢࣜ࢔ࢪࣥࡢỈ࿴จ㞟య࡟ࡘ࠸࡚㉸ᑠゅ X ⥺ᩓ஘ゎᯒࢆ⾜࠸ࢼࣀ–ࢧࣈ࣐࢖ࢡࣟࢫࢣ࣮ࣝ࡟࠾ࡅࡿ㞟ྜᵓ㐀ࡢኚ໬࡟ࡘ࠸࡚ㄪ࡭ࡓࠋࢢࣜ࢔ࢪࣥ⃰ᗘቑຍ࡟క࠸ࠊᩓ஘࣋ࢡࢺࣝ q = 0.13 nm1 ࡟ほ ࡉࢀࡿจ㞟యෆ㒊ࡢ␯ᐦᵓ㐀࡟⏤᮶ࡍࡿᖸ΅ࣆ࣮ࢡࡀῶ⾶ࡍࡿ࡜ྠ᫬࡟ࠊq < 0.1 nm1 ࡢ❧ࡕୖࡀࡾࡀቑ኱ࡋ࡚࠾ࡾࠊỈࡢῶᑡ࡜࡜ࡶ࡟୙ᆒ୍࡞㞟ྜᵓ㐀ࡀจ⦰ࡍࡿ୍᪉࡛඲య࡜ࡋ࡚኱ࡁ࡞จ㞟యࡀᡂ㛗ࡍࡿࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡗࡓࠋ࣮࣮࢟࣡ࢻ㸸 ㉸ᑠゅ X ⥺ᩓ஘ࠊᑠ㯏ࢱࣥࣃࢡ㉁ࠊࢢࣜ࢔ࢪࣥ
⫼ᬒ࡜◊✲┠ⓗ㸸ᑠ㯏⢊㣗ရࡢ≀ᛶࡣࠊᑠ㯏⢊⏕ᆅ୰࡟ྵࡲࢀࡿࢱࣥࣃࢡ㉁ࡢ」ྜయࢢࣝࢸࣥࡢ≀ᛶ࡟ࡼࡗ࡚≉ᚩ࡙ࡅࡽࢀࡿࠋࢢࣝࢸࣥࡣࠊ⢓ᛶⓗ࡞ᣲື࡟ᐤ୚ࡍࡿࢱࣥࣃࢡ㉁ࢢࣜ࢔ࢪࣥ࡜ᙎᛶⓗ࡞ᣲື࡟ᐤ
୚ࡍࡿࢱࣥࣃࢡ㉁ࢢࣝࢸࢽࣥࡢ」ྜయ࡛࠶ࡾࠊᑠ㯏⢊㣗ရࡢ㣗ឤࡢᨵၿࡸຍᕤᛶࡢྥୖࡢࡓࡵ࡟
ࡣࠊࡑࢀࡒࢀࡢࢱࣥࣃࢡ㉁ࡢ≉ᛶࢆ᫂ࡽ࠿࡟ࡍࡿࡇ࡜ࡀ㔜せ࡛࠶ࡿࠋࢢࣜ࢔ࢪࣥࡣࡇࢀࡲ࡛Ỉ࡟୙⁐࡛࠶ࡿ࡜ࡉࢀࠊ60–70%ࡢ࢚ࢱࣀ࣮ࣝỈ⁐ᾮࡸᕼ㓟ࢆ⏝࠸ࡓᢳฟἲ࡟ࡼࡾ༢㞳ࡉࢀ࡚ࡁࡓࠋࡋ
࠿ࡋࠊ㏆ᖺࠊࢢࣜ࢔ࢪࣥࢆ⣧Ỉ୰࡟ᢳฟࡍࡿ᪉ἲࡀぢฟࡉࢀ[1]ࠊᐇ㝿ࡢᑠ㯏⢊⏕ᆅ࡟ࡼࡾ㏆࠸⎔ቃ࡟࠾ࡅࡿࢱࣥࣃࢡ㉁ศᏊࡢᣲືࢆㄪ࡭ࡿࡇ࡜ࡀྍ⬟࡜࡞ࡗࡓࠋࡑࡇ࡛ᡃࠎࡣࠊX ⥺ᑠゅᩓ஘ἲ㸦SAXS㸧ࢆ⏝࠸ࡓࢼࣀᵓ㐀ゎᯒ࡟ࡼࡾ⣧Ỉ୰࡟࠾ࡅࡿࢢࣜ࢔ࢪࣥศᏊࡢᕼⷧ⁐ᾮ୰ࡸ㧗⃰ᗘจ㞟య୰࡛ࡢ఍ྜᵓ㐀࡟ࡘ࠸࡚ㄪ࡭ࠊᕼⷧ≧ែ࡛༢⊂࡛㐟㞳ࡋ࡚࠸ࡓࢢࣜ࢔ࢪࣥศᏊࡀ⃰ᗘࡢቑຍ࡜࡜ࡶ࡟఍ྜࡋ఍ྜࢻ࣓࢖ࣥ㛫ࡢᖸ΅ࣆ࣮ࢡࡀฟ⌧ࡍࡿࡇ࡜ࡸࠊࡉࡽ࡞ࡿ⃰ᗘቑຍ࡟క࠸఍ྜᵓ㐀ࢆ⥔ᣢࡋࡓࡲࡲ୙⁐໬ࡋࢤࣝ≧ࡢจ㞟య࡟࡞ࡿࡇ࡜ࢆぢฟࡍ࡜࡜ࡶ࡟ࠊ㧗⃰ᗘࡢࢤࣝ≧จ㞟య࡟࠾࠸࡚ࠊจ㞟యࡢ␯ᐦᵓ㐀࡟ᇶ࡙ࡃ┦㛵ࣆ࣮ࢡࡀ⾲ࢀࠊࡑࡢ┦㛵ࣆ࣮ࢡࡀ⃰ᗘቑຍ࡟క࠸ḟ➨࡟ῶᙅࡋ࡚࠸ࡃࡇ࡜ࢆ᫂ࡽ࠿࡟ࡋࡓ[2]ࠋ୍᪉ࠊจ㞟యࡢ⃰ᗘቑຍ࡟㝿ࡋ࡚ᑠゅ㡿ᇦࡢ❧ࡕୖࡀࡾࡢቑ኱ࡶㄆࡵࡽࢀࡓࡀࠊほ ࡉࢀࡓኚ໬ࡢ q 㡿ᇦࡀ ᐃୗ㝈್௨ୗ࡛࠶ࡗࡓࡓࡵࠊ኱ࡁ࡞ࢫࢣ࣮
ࣝࡢᵓ㐀ኚ໬࡟ࡘ࠸࡚ࡣ༑ศ࡞㆟ㄽࡀ࡛ࡁ࡞࠿ࡗࡓࠋࡑࡇ࡛ᮏ◊✲࡛ࡣࠊ100–1000 nm ࡢࢫࢣ࣮ࣝࡢᵓ㐀ゎᯒࡀྍ⬟࡞㉸ᑠゅ X ⥺ᩓ஘ἲ㸦USAXS㸧ࢆ⏝࠸࡚ࠊࢢࣜ࢔ࢪࣥจ㞟యࡢࢧࣈ࣐࢖ࢡ࣓࣮ࣟࢺࣝࢫࢣ࣮ࣝࡢᵓ㐀ࢆほ ࡋࠊࢼࣀ࣓࣮ࢺࣝࢫࢣ࣮ࣝࡢᵓ㐀࡜ྜࢃࡏ࡚ࠊ⃰ᗘኚ໬ࡸ㣗ሷῧຍ࡟క࠺ᵓ㐀ኚ໬࡟ࡘ࠸࡚ㄪ࡭ࡓࠋᐇ㦂㸸ヨᩱ࡜࡞ࡿࢢࣜ࢔ࢪࣥࡣ᪤ሗ࡟ᇶ࡙ࡁḟࡢࡼ࠺࡟ㄪ〇ࡋࡓ[1]ࠋᑠ㯏⢊㸦᪥Ύ〇⢊ SuperKingTM㸧
100 g ࡟ 0.5 M 㣗ሷỈࢆ 67 mL ῧຍࡋ 20 ศ㛫ᶵᲔΰᤐࡍࡿࡇ࡜࡟ࡼࡾᑠ㯏⢊⏕ᆅࢆᚓࡓࠋ500 mL⣧Ỉ୰࡛Ὑᾮࢆ஺᥮ࡋ࡞ࡀࡽࡇࡢ⏕ᆅࢆ⧞ࡾ㏉ࡋᥣࡳὙ࠸ࠊ3–6 ᅇ┠ࡢὙᾮࢆᅇ཰ࡋࡓᚋࠊ㣗ሷࢆῧຍࡋࢢࣜ࢔ࢪࣥࢆỿẊࡉࡏࡓࠋ㐲ᚰỿ㝆࡟ࡼࡾᅇ཰ࡋࡓࢢࣜ࢔ࢪࣥࢆ⣧Ỉ୰࡛㏱ᯒࡋࡓᚋࠊ෾⤖஝⇱ࡋ࡚⢊ᮎ≧ࡢࢢࣜ࢔ࢪࣥࢆᚓࡓࠋ ᐃ࡟⏝࠸ࡿヨᩱࡣࠊᡤᐃࡢ⃰ᗘ࡜࡞ࡿࡼ࠺࡟⣧Ỉ㸦ࡲࡓࡣ㣗ሷỈ㸧࡜ΰ࿴ࡋࡓᚋ 4 ᪥⤒㐣ࡋࡓࡶࡢࢆ ᐃ࡟⏝࠸ࡓࠋ
USAXS ᐃࡣࠊSPring-8 ࡢ⏘ᴗ฼⏝ࣅ࣮࣒ࣛ࢖ࣥ I BL19B2 ࡢᴟᑠゅ X ⥺ᑠゅᩓ஘⿦⨨ࢆ฼⏝ࡋ࡚⾜ࡗࡓࠋヨᩱࡣ⭷ཌ 7 ȝm ࡢ࣏ࣜ࢖࣑ࢻࣇ࢕࣒ࣝࢆ❆ᮦ࡜ࡋࡓ PTFE 〇ࡢࢭࣝ࡟඘ሸࡋࠊヨᩱཌࡉࡣ 0.5 mm ࡜ࡋࡓࠋධᑕ X ⥺ࡢ࢚ࢿࣝࢠ࣮ 18 keVࠊ࣓࢝ࣛ㛗 42 m㸦ࢥ࣮ࣛࢤࣥヨᩱࡢᅇᢡࣆ࣮ࢡࢆᇶ‽࡟㍑ṇ㸧࡛ ᐃࢆ⾜࠸ࠊ2 ḟඖ᳨ฟჾ PILATUS 2M㸦DECTRIS㸧ࢆ⏝࠸࡚ᩓ஘ࣃࢱ䠉㻞㻞㻤䠉SPring-8/SACLA ฼⏝◊✲ᡂᯝ㞟Section B࣮ࣥࢆྲྀᚓࡋࡓࠋ㟢ග᫬㛫ࡣ୺࡟ 300 ⛊࡜ࡋࠊᐊ ࡛ ᐃࢆᐇ᪋ࡋࡓࠋ ᐃࡉࢀࡓ 2 ḟඖᩓ஘ࣃࢱ࣮ࣥ࡟ࡘ࠸࡚෇⎔ᖹᆒ࡟ࡼࡿ 1 ḟඖ໬ฎ⌮ࢆ⾜ࡗ࡚ᚓࡽࢀࡓᩓ஘ࣉࣟࣇ࢓࢖ࣝࢆࡶ࡜࡟ゎᯒࢆ⾜ࡗࡓࠋ⤖ᯝ࠾ࡼࡧ⪃ᐹ㸸
ᅗ 1 ࡟⃰ᗘࡢ␗࡞ࡿࢢࣜ࢔ࢪࣥỈ࿴จ㞟య࡟ࡘ࠸࡚ ᐃࡋࡓ USAXS-SAXS ࣉࣟࣇ࢓࢖ࣝࢆ♧ࡍࠋ௒ᅇ SPring-8 ࡟࠾࠸࡚ᚓࡽࢀࡓ USAXS ࡢ ᐃ⤖ᯝ࡟ຍ࠼࡚ࠊ㧗࢚ࢿࣝࢠ࣮ຍ㏿ჾ◊✲ᶵᵓ
Photon Factory BL-10C ู࡛㏵ ᐃࡋࡓ୰㧗ゅ㡿ᇦࡢ SAXS ࡢ ᐃ⤖ᯝࢆࠊྠ୍ヨᩱ࡟ࡘ࠸࡚㐃⥆ࡍࡿࡼ࠺࡟⾲♧ࡋࡓࠋ25%ࡢ⃰ᗘ࡟࠾࠸࡚ࡣࠊ0.14 nm1 ௜㏆࡟ࣈ࣮ࣟࢻ࡞ࣆ࣮ࢡࡀ᫂░࡟ほ ࡉࢀࡓࠋ୍⯡࡟ࠊᩓ஘࣋ࢡࢺࣝ q ࡟࠾࠸࡚ほ ࡉࢀࡿᩓ஘ࡣࠊ┦㛵㊥㞳 d = 2ʌ/q ࡟ᑐᛂࡍࡿᵓ㐀࡜㛵㐃௜ࡅࡽࢀࡿ[3]ࠋ0.14 nm1 ௜㏆ࡢࣆ࣮ࢡࡣ┦㛵㊥㞳 d = 43 nm ࡟ᑐᛂࡋ࡚࠾ࡾࠊࢢࣜ࢔ࢪࣥจ㞟యෆ㒊࡟ࡇࡢ┦㛵㊥㞳ࢆᣢࡘᐦᗘࡺࡽࡂࠊࡍ࡞ࢃࡕ␯ᐦᵓ㐀ࡀᏑᅾࡍࡿࡇ࡜ࢆ♧ࡋ࡚࠸ࡿࠋ୍
᪉ࠊq < 0.03 nm1 ࡢ㉸ᑠゅ㡿ᇦ࡟࠾࠸࡚ࡣࠊPorod ๎ Ivq4 ࡟ᚑ࠺❧ࡕୖࡀࡾ[3]ࡀほ ࡉࢀࠊᩘⓒ nm ௨ୖࡢ኱ࡁ࡞จ㞟ᵓ㐀ࡀᙧᡂࡉࢀ࡚࠸ࡿࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡗࡓࠋྠᵝࡢ≉ᚩࡣࢢࣜ࢔ࢪࣥ⃰ᗘ 50%࡟࠾࠸࡚ࡶྠᵝ࡟ほ ࡉࢀࡿࡀࠊ0.14 nm1 ࡟ほ ࡉࢀࡓࣆ࣮
ࢡ࡟ࡘ࠸࡚ࡣࠊhigh-q ഃ࡟ࢩࣇࢺࡋ 0.67 nm1㸦ᑐᛂ┦㛵㊥㞳 9.6 nm㸧࡜࡞ࡗ࡚࠾ࡾࠊࣆ࣮ࢡᙉᗘࡶᑠࡉࡃ࡞ࡗ࡚࠸ࡿࠋࡇࢀࡣࠊ⃰ᗘࡢୖ᪼࡜࡜ࡶ࡟ࠊ␯ᐦᵓ㐀ࡀᑠࡉࡃ࡞ࡗ࡚࠸ࡿࡇ࡜ࢆ♧ࡋ࡚࠾ࡾࠊࡲࡓ඲య࡟༨ࡵࡿ๭ྜࡶῶᑡࡋ࡚࠸ࡿࡇ࡜ࢆ♧ࡋ࡚࠸ࡿࠋࡉࡽ࡟ࠊ⃰ᗘ 70%࡟࠾࠸࡚ࡣࠊ
୰㧗ゅ㡿ᇦࡢࣆ࣮ࢡࡣ࡯࡜ࢇ࡝ㄆ㆑࡛ࡁ࡞࠸⛬ᗘ࡟࡞ࡗ࡚࠾ࡾࠊPorod ๎࡟ᚑ࠺❧ࡕୖࡀࡾࡢࡳࡀほ ࡉࢀࡿࡼ࠺࡟࡞ࡿࠋ௨ୖࡢࡇ࡜࠿ࡽࠊࢢࣜ࢔ࢪࣥจ㞟యࡣࠊ⃰ᗘࡀẚ㍑ⓗᑠࡉ࠸㡿ᇦ࡛ࡣࠊ40 nm ⛬ᗘࡢ␯ᐦᵓ㐀ࢆෆ㒊࡟᭷ࡋࡘࡘ඲య࡜ࡋ࡚኱ࡁ࡞จ㞟యࢆᙧᡂࡋ࡚࠸ࡿࡀࠊඹᏑࡍࡿỈࡢ㔞ࡀῶᑡࡍࡿ࡜࡜ࡶ࡟␯ᐦᵓ㐀ࡀ⦰ᑠࡋࡘࡘኻࢃࢀ࡚࠸ࡁࠊ70%࡟࠾࠸࡚ࡣ࡯࡜ࢇ࡝₽ࢀ࡚ࡋࡲ࠸⦓ᐦ࡞จ㞟ᵓ㐀࡬࡜⛣⾜ࡋ࡚࠸ࡃࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡗࡓࠋ

ᅗ 1. ␗࡞ࡿ⃰ᗘ࡛ ᐃࡋࡓࢢࣜ࢔ࢪࣥỈ࿴จ㞟యࡢ USAXS-SAXS ࣉࣟࣇ࢓࢖ࣝࠋSPring-8 ࡟࠾࠸࡚ ᐃࡋࡓࢹ࣮ࢱ㸦ኴ⥺㸧࡜ Photon Factory ࡟࠾࠸࡚ ᐃࡋࡓࢹ࣮ࢱ㸦⣽⥺㸧ࢆྜ୍ࡋ࡚♧ࡍࠋᅗ୰ࡢᩘᏐࡣࢢࣜ࢔ࢪࣥ⃰ᗘࢆ♧ࡋࠊぢࡸࡍࡃࡍࡿࡓࡵࠊྛࣉࣟࣇ࢓࢖ࣝࢆ⦪᪉ྥ࡟ࢩࣇࢺࡋ࡚⾲♧ࡋ࡚࠸ࡿࠋ◚⥺ࡣࠊPorod ๎ Ivq4 ࡢ㛵ಀࢆ♧ࡍࠋ
ᅗ 2 ࡟ࠊ⃰ᗘ 50%ࡢࢢࣜ࢔ࢪࣥỈ࿴จ㞟య࡟㣗ሷࢆῧຍࡋࡓ㝿ࡢ USAXS-SAXS ࣉࣟࣇ࢓࢖ࣝࡢኚ໬ࢆ♧ࡍࠋ㣗ሷ⃰ᗘ 0 Mࠊࡍ࡞ࢃࡕࠊ⣧Ỉ୰࡟࠾࠸࡚ ᐃࡉࢀࡓᩓ஘ࣉࣟࣇ࢓࢖ࣝࡢ 0.67 nm1
࡟ほ ࡉࢀࡓࣆ࣮ࢡࡣࠊ㣗ሷ⃰ᗘࡢቑຍ࡜࡜ࡶ࡟ࢃࡎ࠿࡟ high-q ഃ࡟ࢩࣇࢺࡍࡿ࡜࡜ࡶ࡟ࠊࣆ࣮
ࢡᙉᗘࡀῶᑡࡋ࡚࠸ࡃࡇ࡜ࡀࢃ࠿ࡿࠋࡇࡢኚ໬ࡣࠊᅗ 1 ࡟♧ࡍࢢࣜ࢔ࢪࣥ⃰ᗘࡢኚ໬ࡢᵝែ࡜㏆ఝࡋ࡚࠾ࡾࠊ㣗ሷࡢῧຍ࡟ࡼࡗ࡚ࠊ␯ᐦᵓ㐀ࡀจ⦰ࡋࡘࡘḟ➨࡟ᾘኻࡋ࡚࠸ࡃࡇ࡜ࢆ♧ࡋ࡚࠸ࡿࠋ䠉㻞㻞㻥䠉SPring-8/SACLA ฼⏝◊✲ᡂᯝ㞟

Section Bࡇࡢࡇ࡜ࡣࠊࢢࣜ࢔ࢪࣥࡢỈ࿴จ㞟య࡟ᑐࡋ࡚ࠊ㣗ሷࡀจ⦰ຠᯝࢆཬࡰࡍࡇ࡜ࢆ⾲ࡋ࡚࠸ࡿࠋ୍
᪉ࠊq < 0.03 nm1 ࡢ㉸ᑠゅ㡿ᇦ࡟࠾࠸࡚ࡣࠊ1 M ࡢ㣗ሷࡢῧຍ࡟కࡗ࡚ࠊ᪂ࡓ࡟ࣈ࣮ࣟࢻ࡞ࣆ࣮ࢡࡀฟ⌧ࡍࡿࡇ࡜ࡀࢃ࠿ࡗࡓ㸦ᅗ୰㉥▮༳㸧ࠋࡇࢀࡣࠊ⣧Ỉ୰࡟Ꮡᅾࡍࡿ 10 nm ๓ᚋࡢ␯ᐦᵓ㐀࡟ຍ࠼࡚ࠊ᪂ࡓ࡟኱ࡁ࡞ࢫࢣ࣮ࣝࡢ␯ᐦᵓ㐀ࡀฟ⌧ࡋࡓࡇ࡜ࢆ♧ࡋ࡚࠾ࡾࠊ㣗ሷࡢจ⦰ຠᯝ࡟ࡼ
ࡗ࡚ࠊ2 ḟⓗ࡞␯ᐦᵓ㐀ࡀᙧᡂࡉࢀࡿࡇ࡜ࢆ♧ࡋ࡚࠸ࡿࠋ㣗ሷ⃰ᗘࡀቑຍࡋ 3M ࡟࡞ࡿ࡜ࠊࡇࡢ
ࣆ࣮ࢡࡣ high-q ഃ࡟ࢩࣇࢺࡋࡘࡘᙉᗘࡀపୗࡋ࡚࠾ࡾࠊ㧗⃰ᗘࡢ㣗ሷᏑᅾୗ࡛ࡣࠊࡼࡾจ⦰ຠᯝࡀᙉࡃാࡁࠊ2 ḟⓗ࡞␯ᐦᵓ㐀ࡶ཰⦰ࡋᾘኻࡋ࡚࠸ࡃࡇ࡜ࡀࢃ࠿ࡿࠋࡇࢀࡽࡢኚ໬ࡣࠊ㣗ሷࡢῧຍ࡟ࡼࡗ࡚ࢢࣜ࢔ࢪࣥࡢỈ࿴≧ែࡢኚ໬ࡀ⏕ࡌࡿࡇ࡜࡟ࡼࡗ࡚ࡶࡓࡽࡉࢀࡿࠋࡍ࡞ࢃࡕࠊῧຍࡋࡓ࢖࢜ࣥ࡟ࡼࡗ࡚ࠊࢢࣜ࢔ࢪࣥࡢỈ࿴Ỉࡢ཰ዣࡸ㟼㟁㐽ⶸ࡟ࡼࡿࢢࣜ࢔ࢪࣥศᏊ㛫ࡢ᩺ຊపῶࡀ⏕ࡌࠊࢢࣜ࢔ࢪࣥศᏊ㛫ࡢ␯Ỉᛶ┦஫స⏝࡟ࡼࡿจ㞟ࢆ⏕ࡌࡸࡍࡃ࡞ࡿࡓࡵ࡛࠶ࡿ࡜⪃࠼ࡽࢀࡿࠋ

ᅗ 2. ࢢࣜ࢔ࢪࣥ⃰ᗘ 50%ࡢỈ࿴จ㞟య࡟ࡘ࠸࡚␗࡞ࡿ⃰ᗘࡢ㣗ሷࢆῧຍࡋࡓ㝿ࡢ USAXSSAXS ࣉࣟࣇ࢓࢖ࣝࠋSPring-8 ࡟࠾࠸࡚ ᐃࡋࡓࢹ࣮ࢱ㸦ኴ⥺㸧࡜ Photon Factory ࡟࠾࠸࡚ᐃࡋࡓࢹ࣮ࢱ㸦⣽⥺㸧ࢆྜ୍ࡋ࡚♧ࡍࠋᅗ୰ࡢᩘᏐࡣ㣗ሷ⃰ᗘࢆ♧ࡋࠊぢࡸࡍࡃࡍࡿࡓࡵࠊྛࣉࣟࣇ࢓࢖ࣝࢆ⦪᪉ྥ࡟ࢩࣇࢺࡋ࡚⾲♧ࡋ࡚࠸ࡿࠋ◚⥺ࡣࠊPorod ๎ Ivq4 ࡢ㛵ಀࢆ♧ࡍࠋ௒ᚋࡢㄢ㢟㸸㣗ሷῧຍ᫬ࡢ㉸ᑠゅ㡿ᇦࡢࣆ࣮ࢡࡢฟ⌧࡜ࢩࣇࢺࢆࡶࡓࡽࡍ 2 ḟⓗ࡞␯ᐦᵓ㐀ᙧᡂ࡟ࡘ࠸࡚ࡣࠊ㣗ሷ⃰ᗘࡸ࢖࢜ࣥࡢ✀㢮ࡢࣂ࢚࣮ࣜࢩࣙࣥࢆቑࡸࡍࡇ࡜࡟ࡼࡗ࡚ࠊࡼࡾヲ⣽࡟㏣㊧ࡍࡿᚲせࡀ࠶ࡿࠋࡇࢀ࡟ࡼࡗ࡚ࠊࢢࣜ࢔ࢪࣥศᏊࡢᙧᡂࡍࡿ㝵ᒙⓗ࡞㞟ྜᵓ㐀ࢆ᫂ࡽ࠿࡟ࡍࡿ࡜࡜ࡶ࡟ࠊ࢖࢜ࣥࡢ✀㢮ࡸ㔞ࡀࢢࣜ࢔ࢪࣥࡢỈ࿴≧ែ࡟ཬࡰࡍᙳ㡪ࢆࡼࡾヲ⣽࡟ゎ᫂ࡍࡿࡇ࡜ࡀྍ⬟࡜࡞ࡿࠋཧ⪃ᩥ⊩㸸[1] T. Ukai et al., J. Agric. Food Chem., 56, 1122 (2008).
[2] N. Sato et al., J. Agric. Food Chem., 63, 8715 (2015).
[3] D. I. Svergun and M. H. J. Koch, Rep. Prog. Phys., 66, 1735 (2003).

(Received: September 29, 2018; Early edition: May 9, 2019,
Accepted: July 16, 2019; Published: August 29, 2019䠉㻞㻟㻜䠉