BIO XTM

WE CREATE THE FUTURE OF HEALTH

What is Bioink?
A bioink is a biomaterial that is suitable for bioprinting with cells andprovides a temporary or permanent support to the cells while they
produce their own extracellular matrix. Bioinks based on biopolymers,such as collagen, gelatin, hyaluronan, silk, alginate and nanocellulose,
are known for their favorable biocompatible properties and are attractive biomaterials for cell encapsulation and 3D bioprinting. Thesebioinks provide an aqueous 3D environment with biologically relevant
chemical and physical signals, mimicking the natural extracellular matrix environment. Significant advances in 3D bioprinting technology as
well as the development of new bioinks have made it possible to bioprint complex 3D tissue structures.

Why Bioprinting?
The innovative methods for engineering human tissues and organs
can have a profound effect on the future of medicine. 3D bioprinting
is considered a revolutionizing technology for advancing and accelerating progress in the field of tissue engineering and regenerative medicine, and thus, the future of medicine. We believe that we can create
this future through a collaborative spirit and by putting our combined
expertise to the service of humanity.
The future is created in the present and it belongs to the doers, those
who continue moving forward in order to see their vision come to realization. It’s not that we see the future and then move towards it. We
move in order to see it.

We are CELLINKWe are a team of entrepreneurs, scientists, engineers and pioneers, pushing thelimits for what’s possible, paving the way for the future of regenerative medicine.
With our 3D bioprinters and bioinks, we will open the possibility for more extensive medical research. Together with our collaborators,in hundreds of labs inmore than 50 countries, we work side by side to ensure quality and support.
Our compassion for humans and drive to create an impact will pave the way forcontinued growth.

“There was a clear difference in
CELLINK’s customer approach,product robustness and confidence
in their products, all of which madeCELLINK stand alone in the marketof 3D bioprinting. We trusted thecompany and now looking back wethink it was not a wrong decision togo with CELLINK.”- Dr. Nath, Harvard Medical School“Thanks CELLINK for engaging thestudents and holding this successful
workshop on bioprinting”- Ric Levato, UMC Utrecht“CELLINK has taken our feedback
and adapted their system while being actively engaged in the process”- Dr. Grande, The Feinstein Institute for
Medical ResearchMeet the BIO X
BIO X is the most user-friendly and flexible bioprinter in the world, providing theuser with an unparalleled bioprinting experience. The built-in features along withthe new BIO X software managed through the large touchscreen display minimize the learning curve and ensure you will receive the results you want.
Bioprinted tissue can be used in drug discovery where researchers can test new
potential treatments and evaluate efficacy in very early stages. New drugs andtreatments will potentially reach clinical trials faster with a decreased numberof failures and reduced need for animal testing.
BIO X is the next-generation bioprinter, bringing scientists closer to the futureof medicine.

Wider printpressure range

Better print
resolution

1700kPa

BIO X

400 kPa
Before(365, 405, 485 and 520 nm UV LED)

Temperature
control

37-60

7printheadprintbed°C

mountsµm

BIO X

Multihead builds
More printheads

2 mounts

10 µm

printheads/toolheads

1 printhead

BeforeBIO X

Before4-250°C

BIO X

Room temp
printbed

20-130 °C
printhead

BeforeDesigned withscientists in mind
YOU SPOKE, WE LISTENED!
When developing the BIO X, we at CELLINK reached out to you, our fellow scientists and users, to get your feedback on our INKREDIBLE and INKREDIBLE+
bioprinters, and to better understand your needs in 3D bioprinting.
We asked what current features you would want to improve, and what non-existing features you’d like to see in the next-generation bioprinter. You can findthe most common answers in the diagram below.

Temperature control
Easier to adjust precisely
Wider print pressure range
Better display
Multihead builds
Better print resolution
Predictability of nozzle start position
More printheads
Repeatability/dimensional accuracy
Better filter/cleaner
More inks
Other01020%

HumanBIO XCompressor

INDEPENDENT AND COMPACT LAB FOOTPRINT
Both a compressor and cooling unit have been integrated into the BIO X, without compromising its size. When printing cells, a sterile environment is key.
Working in a laminar flow cabinet is sometimes needed, but but impossible todo if your equipment can’t fit. BIO X has a small footprint but still contains everycomponent you need to bioprint. It’s a complete standalone unit, facilitating
work in a laminar flow cabinet without the need for external connections.
Even though the BIO X works perfectly on its own, you might sometimes want
to increase performance of different functions. There’s a plug in the back of theBIO X where you can connect to your laboratory air supply, if needed. Connecting the external air supply allows you to print with higher pressure than usual,enabling you to print higher viscosity bioinks.

THERMOPLASTIC

COOLED

ELECTROMAGNETICSYRINGE PUMPPHOTO

HD CAMERAEXTRUDER

PNEUM AT ICDROPLE T PRINTHEDPRINTHEADCURING TOOL

TOOL HEAD

HEAD

HEAD

Intelligent & exchangeable
To make sure that all the necessities for your research are easily available, wesupply several of the handiest printheads and tool heads you may need whenusing BIO X.
BIO X is a versatile bioprinter equipped with intelligent printhead mounts. Thismeans you’ll have the opportunity to easily upgrade your system as we develop
new printheads to match your evolving bioprinting needs.
The printheads we provide are carefully selected and of the highest quality. We
strive to meet your standards and we compromise nothing in the process ofsupporting your bioprinting projects.

Compatible materials
The BIO X is capable of fabricating constructs containing any type of cell, enabling
the production of any tissue target found in the body. These constructs can beutilized in a wide range of applications through the incorporation of bone marrowstromal cells or mesenchymal stem cells.
The system can use specialized cells like chondrocytes or fibroblasts for cartilage
and dermal applications respectively. Build vascular networks within your constructs using.
BIO X can bioprint hepatocytes to rapidly create 3D models for drug screening. You
can add stellate cells to quickly make advanced disease models.

LIST OF BIOINKS AND THEIR PRINTING EXTRUSION METHODS
Pneumatic-drivenExtrusion

Piston-driven Extrusion(syringe)(heated)(heated)Gelatin Methacryloyl
Collagen methacryloyl(Collagen solution and precipitated)

Hyaluronan
Alginate
ChitosanSilk
Nanocellulos e
PEG/PEGDA
Fibrinogen/th rombin
Decellularized ECM
Pluronics F127
PropyleneG lycol
Polycaprolactone
Polylacatic Acid

Inkjet

Thermoplastic
ExtrusionClean. Reinvented
BIO X is equipped with dual high-power fans that create a powerful airflow
through its dual-filtration top, creating a positive air pressure inside thechamber. The air first travels downwards through a prefilter, which retains thebigger particles, and then through a HEPA H14 filter, which sorts out even thesmallest of particles. The dual- power fans fill the chamber with filtered air at
a positive pressure to keep your experiment clear.
The BIO X is made without sharp corners. Its design only uses rounded shapes
to ensure that no unwanted particles get trapped inside the chamber. On topof this, there are UV-C germicidal lights that allow you to run sterilization cycles to sterilize the printing environment. Together, these components create acomplete system of uncompromised cleanliness.

Easy printing process 321

123

CELL MIXING
We have developed the easiest and most homogenous wayof doing this using our innovative CELLMIXER. Put the bioink
in the 3 mL syringe and your cells in suspension media in the
1 mL syringe. Clip each syringe to the dispensing unit, connect
the mixing unit to the tip of each syringe and then connect thefilling-cartridge. Fill the cartridge by gently injecting the ink andcells through the mixing unit. Your filling-cartridge is now readyfor bioprinting and can be disconnected from the mixing unit.

BIOPRINTINGWhen the cell mixing is done and your cartridge is filled, you’reready to start pinting. Screw a nozzle onto the cartridge andconnect it to the air system. Now place it in the printhead.
Continue by choosing the desired printing settings on thetouchscreen, like temperature, printing pressure and printing
speed. The parameters and the nozzle’s diameter are chosen
based on your chosen material. Select the design you want andpress print. BIO X will calibrate itself and start printing.

CROSSLINKING
Depending on the material you are printing, you may need tocrosslink the printed construct. For UV crosslinking, you canturn on the built-in LED and the BIO X will do all the work foryou. For other types of crosslinking, you can add the crosslinking agent directly on your construct.

Our bioinks
CELLINK is the first bioink company in the world and the creator of the world’s
first universal bioink. This opens the opportunity for scientists to bioprint using
any type of cells.
Today we provide more than 40 different sterile and ready-to-use bioinks forvarious applications, from printing cancer models to skin models. Moreover, it iscompatible with any 3D bioprinting system.
You can also check out our different kits for special applications and needs. TheSupport kit will enable the fabrication of your 3D constructs from otherwise
unprintable materials and revolutionize your research.
BioinkCELLINK A

Cartilage√

CELLINK A - RGD
CELLINKSkinBone√√√

Muscle√CELLINK BONE

MSCs

Other celltypes

Thermoplastic
Scaffolds

√
√

√

√

√

√√

√

√

CELLINK FIBRINOGEN√√√√CELLINK FIBRIN√√√√

CELLINK RGD√√√√CELLINK SKIN√

CELLINK SKIN+√CELLINK LAMININK 111√

CELLINK LAMININK 121√√

CELLINK LAMININK 411√

CELLINK LAMININK 521√

CELLINK LAMININK+√Coll1√√√√√CollMA√√√√√GelMA√√√√√

GelMA A√√√√√

GelMA C√

√√√√

√

√

GelMA high C√√√√√GelXA√√√√√

GelMA HA

Sacrificialmaterial√

GelXA-Bone√√GelXA-Fibrin√

GelXA-Skin

√

√√√

√

GelXA-LN111√GelXA-LN121√√

GelXA-LN411√GelXA-LN521√GelXA-LN+√

GelXG√√√√√CELLINK PCL√

PLA√

PLGA√

CELLINK Pluronics√

CELLINK START√CELLINK START X√

CELLINK Support√

CELLINK Xplore
HAMA Kit√√√Bioverse.co
EXTENDING DEVELOPMENT BEYOND YOUR LAB
Join the global open-source 3D bioprinting community online. You’ll connect withgreat minds around the world, streamlining your work and driving innovation.
Bioverse.co also gives you access to CAD models and protocol for all types of organs, tissues and tissue analogues. Register your products to get notified whenyour warranties are about to expire, if maintenance should be done and whensoftware updates become available.
Bioverse.co is developed and maintained by CELLINK AB.

OWNERSHIP HAS NEVER BEEN MORE CONVENIENT
Bioverse.co is not only a forum for sharing, but also for caring. Use your products’ serial numbers and register them under your account at Bioverse. You’ll get
instant notifications when your warranties are about to expire, if maintenanceshould be done and when there are new software updates for your BIO X.
Connect your BIO X to the internet and download 3D models from Bioverse
directly. If the downloaded project files contain printing protocols, your BIO X will
use them to set up automatically.

COLLABORATE TO ACCELERATE PROGRESS
Search through this online database for human organ models, tissue models orprotocols to improve your work. Extend your expertise beyond your lab by collaborating with other great minds around the world.

www.cellink.com | sales@cellink.com | US: +1 (833) CELLINK | EU: +46 31-128 700

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