/&FROXPQFDWDORJ
5HYHUVHGSKDVHFROXPQ/&FROXPQFDWDORJ/FROXPQ/FROXPQ/FROXPQ6XSHUSHUIRUPDQFHFROXPQIRU/&6LQFH

9HU

L-column3

L-column, L-column2- Feature- Feature- UHPLC column- Line up- 0HWDOIUHHFROXPQ- $SSOLFDWLRQGDWD- 7HFKQLFDOUHSRUW- /LQHXSRealizing both excellent chemical stability
and top-level low adsorptivity
Unparalleled high-performance, all-around column

3%RQGHGSKDVH&&

3&6VLOLFD(PHUIHFWFKHPLFDOVWDEOHVLOLFD

3HUIHFWVWDEOHHQGFDSSLQJŶ,PSURYLQJFKHPLFDOVWDELOLW\VRWKDWXVDEOHZLWKLQWKHUDQJHRIS+WRS+Ŷ/RZDGVRUSWLYLW\LVDWWKHWRSOHYHOIRUYDULRXVDQDO\WHVŶ,PSURYHPHQWRIVHSDUDWLRQRUSHDNVKDSHVLQFHWKHS+RIDQHOXHQWLVIOH[LEO\VHOHFWDEOHŶ$DTXHRXVHOXHQWFDQEHVWDEO\DQDO\]HG

1

Silica-based columns with the many advantages of high separation efficiency and mechanical strength are currently usedmost generally for the columns for reversed phase chromatography. On the other hand, the pH range of usable eluent islimited because exposure to the alkaline solution are easily dissolved silica.
L-column3 is an all-around column where the chemical stability (acid and alkaline resistance) of the packing materials is
dramatically improved by using newly developed and highly alkaline resistant PCS silica (perfect chemical stable silica)
and perfectly stable end-capping with performance much higher than that of L-column2.

L-column3 proposes the possibility of a new analysis.

L-column3 C18
Base materials

L-column3 C8

PCS silica (Perfect chemical stable silica)

Pore size

12 nmSpecific surface area

340 m2/g

Particle sizeȝPȝPȝP

Bonded phase

Octadecyl silyl (C18, ODS)

End-capping
USP category
Usable pH rangeȝPȝP
Octyl silyl (C8, Octyl)

Perfect stable end-capping
L1

L7
pH 1 - pH 122

L-column3

L-column3 is chemically stable, so that usable within the range of pH 1 to pH 12.
L-column3 offers very high resistance to alkaline, and packing materials are designed to be robust. In addition, it performs similar highresistance to neutrality and acidity.
L-column3 can be used as an all-around column capable of method design for a wide range of pH values.
1

2

3

4

5

6

7

8

9

10

11

12

L-column2 pH 1 - pH 9
L-column3 pH 1 - pH 12

140
Stability of Retention data (%)

Stability of Retention data (%)

140k (Amitriptyline) / k (Acenaphthene)

130

120

L-column2

110

L-column3

100
0

100

200
Time (Hour)

300

k (Amitriptyline) / k (Acenaphthene)

L-column2

120

110

L-column3

100

400

0

Ŷ'XUDELOLW\WHVW(pH 1)

50

100
150
Time (Hour)

200

250

- k : Retention factor

Stability of N (Naphthalene) (%)

120[Durability test conditions]&ROXPQ&ȝPSize: 2.1 mm I.D., 150 mm L.
Eluent: CH3OH/1% TFA in H2O (10/90))ORZUDWHP/PLQTemp.: 40°C

Amitriptyline

130

L-column3
100

80Brand A
Brand B
Brand C
Brand E
Brand F

60

40
0Acenaphthene

L-column2

50

100
150
Time (Hour)

200

250

Ŷ'XUDELOLW\WHVW(pH 12, triethylamine solution)
H3C

N
CH3

[Durability test conditions]&ROXPQ&ȝPSize: 2.0 mm or 2.1 mm I.D., 150 mm L.
Eluent: CH3OH/54 mM TEA in H2O (10/90))ORZUDWHP/PLQ7emp.: 50°C
- k : Retention factor, N : Number of theoretical platesComparison columns are as follows:- Columns of other company: Usable up to pH 12.
- L-column2: Usable from pH 1 to pH 9.

120
Stability of N (Naphthalene) (%)

An alkaline eluent promotes the degradation of silica-based
columns. The dissolution of silica adds vacant space to thecolumn bed and decreases the number of theoretical plates.
The loss of the bonded phase and exposure of the silanolgroup over the surface of silica cause adsorption of basic
compounds into the silanol group, which results in a delay ofretention (see the technical report on P. 11). A strong acideluent is also subject to similar degradation, although thechange is slow.
L-column3 shows small changes and is stable in durability
tests at pH 1 and pH 12 (triethylamine solution andphosphate buffer solution).

L-column3
100

80

60

L-column2
40
0

50

200

250

Ŷ'XUDELOLW\WHVW S+SKRVSKDWHEXíHUVROXWLRQ[Durability test conditions]&ROXPQ&ȝPSize: 2.1 mm I.D., 150 mm L.
Eluent: CH3OH/10 mM Phosphate buffer (10/90))ORZUDWHP/PLQ7emp.: 40°C
- N : Number of theoretical plates

3

100
150
Time (Hour)L-colum
L-column3

Low adsorptivity is at the top level in various analytes.
L-column3 shows a sharp peak not only for basic compounds but also for acidic compounds and coordination compounds.
L-column3 demonstrates low adsorptivity at the top level for various analytes and provides an ideal peak shape.
ŶBasic compounds

Eluent: CH3CN/25 mM Phosphate buffer pH 7 (35/65)
1. Paroxetine

2. Citalopram

F

NH
H
O

O

ŶCoordination compound

Eluent: CH3CN/20 mM H3PO4 in H2O (2/98)

Eluent: CH3CN/20 mM H3PO4 in H2O (60/40)

1. Formic acid

3. Fluoxetine
H3C
NH

N
O

H

ŶAcidic compounds

O

CF3

H

N CH3
H3C

O

2. Acetic acid

OH

H3C

2. Hinokitiol

1. Uracil
O

O

O

OH

OH

NH
N
H

O
CH3
H3C

F

S(3) : 1.30

L-column3

S(1) : 1.20

1S(2) : 1.08

3

1

3

2

5

10
7LPH PLQ

S(3) : 1.29

L-column2

2

1

2

0

3. Benzene

O

2

15

20

0

1

2
7LPH PLQ

3

4

0

S(1) : 1.17

3

4

8
7LPH PLQ

16

12

16

12

16

12

16

S(2) : 1.06
1

1

12

2

3

1
2

0

Brand A

5

10
7LPH PLQ

15

20

0

S(3) : 3.76 2

1

2
7LPH PLQ

3

4

0

S(1) : 4.17

48
7LPH PLQ

S(2) : 1.21
2

2

1

3

3

1
1
0

Brand C

5

10
7LPH PLQ

15

20

0

S(3) : 1.69

1

S(1) : 1.24

2
7LPH PLQ

3

4

0

4

S(2) : 3.83

1

2

Brand D

5

3

2
1

3

1

0

8
7LPH PLQ

2

10
7LPH PLQ

S(3) : 1.21

15

20

0

1

2
7LPH PLQ

3

1

2

4

0

S(1) : 1.13

2
3

1

4

8
7LPH PLQ

S(2) : 1.29
1
3

2

0

Brand E

5

10
7LPH PLQ

S(3) : 1.60

15

20

0

1

2
7LPH PLQ

3

4

0

1

2

3

1

8
7LPH PLQ

1216

S(2) : 1.05

S(1) : 1.15

2

4

1
3
2

0

5

10
7LPH PLQ

15

20

0

1

2
7LPH PLQ

3

4

0

4

8
7LPH PLQ

1216[Analytical conditions] &ROXPQ&ȝPSize: PP,'PP/)ORZUDWHP/PLQ7HPS°C,QMYROȝ/- S : Symmetry factor

4L-column3

Improvement of separation and peak shape, since the pH of an eluent is flexibly
selectable.
Widening of the pH range of a usable eluent remarkably enlarges the range of the method of analysis. The use of an alkaline eluent,especially, enables analyses where the dissociation of basic compounds is restricted, which is very advantageous.
16

7. Triprolidine


S+


12








S+













7LPH PLQ

2. Ibuprofen(Acidic
compounds)

1. ketoprofen

0

pH

4

6
(pH)

8

10

12

- The polygonal line graph is showed the retention behavior. Actually, retention timenever linearly changes because of the existence of the buffer capacity or the
influence of the buffer salt.

Amitriptyline

H3C

High

(pKa 9.4)
NCH3

5 mM NH3 in H2O
5 mM HCO3NH4 in H2O
5 mM CH3COONH4 in H2O
5 mM HCOONH4 in H2O
0.1% HCOOH in H2O
8

12Time (min)ŶComparison of retention time (Basic drugs)[Analytical conditions]
Column: L-column3 C18, ȝPSize: 2.1 mm I.D., 150 mm L.
Eluent: A: CH3CN, B: Aqueous solution
A/B, 40/60-90/10-90/10 (0-10-12 min)Flow rate: 0.3 mL/minTemp.: 40°CDetection: ESI-MS/MS (+)
Inj. vol.: ȝ/

Peak intensity is important in the LC/MS often used in trace
analysis. The high pH of an eluent in the analysis of basic
compounds provides sufficient retention and peak intensity.
This is the advantage of the use of an alkaline eluent.

5

3. Indometacin

2

Sufficient retention
Increase of peak intensity

4

4. Isopropyl 4-hydroxybenzoate(Weak acidity compounds)

8[Analytical conditions]
Column: L-column3 C18, ȝPSize: 2.1 mm I.D., 150 mm L.
Eluent: A: CH3CN, B: 25 mM Phosphate buffer
A/B, 20/80-70/30 (0-20 min)Flow rate: 0.3 mL/minTemp.: 40°C'HWHFWLRQ89QP
Inj. vol.: ȝ/

0

5. Fexofenadine(Amphoteric compounds)

4ŶComparison of retention time (Drugs)/RZ(Basiccompounds)





S+









6. Fluvoxamine

Retention time (min)

The change in the pH of an eluent changes the retention
behavior of ionic compounds. Generally, for acidic
compounds, higher pH results in less retention. Conversely,
in basic compounds, higher pH results in greater retention.
There is a strong possibility that the use of an alkaline eluent
provides a separation pattern different from the acidity andneutral ranges, thus an improvement in separation can be
expected.

L-colum
L-column3

The preparation efficiency of basic compounds is improved by using an alkaline
eluent.
7KHXVHRIDQDONDOLQHHOXHQWUHPDUNDEO\LPSURYHVWKHSHDNVKDSHRIEDVLFFRPSRXQGV(YHQDODUJHLQMHFWLRQYROXPHGRHVQRWFDXVH
deviations in the retention time but provides a sharp peak. Consequently, the increase in the loads becomes possible, and preparation
efficiency is improved.
Homochlorcyclizine

N

CH3

N

pH 11

1XPEHURISHDNV 51

pH 7

pH 7

1XPEHURISHDNV 44

pH 2

pH 2

1XPEHURISHDNV 45

H

pH 11

0

Cl

5

10

15
20
Time (min)

25

30

35

0

Ŷ&RPSDULVRQRILQMHFWLRQYROXPH %DVLFGUXJV

5

15
20
Time (min)30

35[Analytical conditions]
Column: L-column3 C18, ȝPSize: PP,'PP/
Eluent: A: CH3CN, B: 25 mM Phosphate buffer$% PLQ)ORZUDWHP/PLQTemp.: 40°C'HWHFWLRQ89QP,QMYROȝ/6DPSOH+RPRchlorcyclizine

7KHJ/KRPRFKORUF\FOL]LQHDGGHGWKHK\GURJHQSHUR[LGHDQGOHIWIRUKRXUVLV
used.

7KHJ/KRPRFKORUF\FOL]LQHDGGHGWKHK\GURJHQSHUR[LGHDQGOHIWIRUKRXUVLV
used.

Alkaline eluent

Alkaline eluent

10 mM HCOOH+NH3 in H2O (pH 11),QMYROȝ/ J/
Duloxetine

Scale-up/RDGȝJ

5

10 mM HCOOH+NH3 in H2O (pH 11)&ROXPQ6L]HPP,'PP/
)ORZUDWHP/PLQ,QMYROȝ/ J/ 

Inner diameter of column
Flow rate
Injection volume
Sample concentration
10

15

0

5

Purity check:
Before purification 98.2%

Neutral eluent

Acidic eluent
10 mM HCOOH in H2O (pH 3),QMYROȝ/ J/

10

15Time (min)Time (min)Preparation

10 mM HCOOH+NH3 in H2O (pH 7),QMYROȝ/ J/

After purification
/RDG
ȝJ

25

Ŷ&RPSDULVRQRIWKHQXPEHURISHDNV %DVLFGUXJV[Analytical conditions]
Column: L-column3 C18, ȝPSize: PP,'PP/
Eluent: A: CH3CN, B: 25 mM Phosphate buffer$% PLQ)ORZUDWHP/PLQTemp.: 40°C'HWHFWLRQ89QP,QMYROXSSHUȝ/ORZHUȝ/6DPSOH+RPRchlorcyclizine

0

10

/RDG
ȝJ

Duloxetine

Duloxetine>99.9%

Duloxetine
H
N

O
S

5

10
Time (min)

15

5

10
Time (min)Ŷ3UHSDUDWLYHSXULILFDWLRQ %DVLFGUXJV[Analytical conditions]
Column: L-column3 C18, ȝPSize: PP,'PP/
Eluent: A: CH3CN, B: Aqueous solution$%*UDGLHQWHOXWLRQ)ORZUDWHP/PLQTemp.: 40°C'HWHFWLRQ89QP
Sample: Duloxetine

15(pKa 8.1)

7KHXVHRIDQDONDOLQHHOXHQWSURYLGHVWKHIROORZLQJDGYDQWDJHVLQEDVLFFRPSRXQGV/RDGLQFUHDVHVDQGSUHSDUDWLRQHIILFLHQF\LVLPSURYHG- Capable of preparation without counterion
,PSURYHPHQWLQWKHVHSDUDWLRQRIWKHGHJUDGDWLRQSURGXFW
or impurities.

6L-column3

A 100% aqueous eluent is available for stable analysis.
7KHGHWHULRUDWLRQRIWKHVLOLFDEDVHGFROXPQLVSURPRWHGZKHQXVHGIRUDDTXHRXVHOXHQWIRUDORQJSHULRGRIWLPH
6LQFHWKHSDFNLQJPDWHULDOVIRUL-column3KDYHYHU\KLJKFKHPLFDOVWDELOLW\WKHFROXPQLVVWDEOHIRUORQJSHULRGVRIWLPHHYHQ
ZKHQXVHGIRUWKHDTXHRXVHOXHQW.
120
Stability of N 3\ULGR[LQH  

L-column3

L-column3

1

2

100

3

0 Hour80

250 HourL-column2

60

0

2

4
6
7LPH PLQ

40
0

50

100
150
7LPH +RXU

200

250

L-column2

8

10

1
2
3

ŶDurability test
0 Hour[Durability test conditions]&ROXPQ&ȝPSize: 2.1 mm I.D., 150 mm L.
Eluent: 25 mM Phosphate buffer pH 7
)ORZUDWHP/PLQ7HPS°C

250 Hour- N : Number of theoretical plates

0

2

4
6
7LPH PLQ

810

Ŷ9LWDPLQ%
[Analytical conditions]&ROXPQ&ȝPSize: 2.1 mm I.D., 150 mm L.
Eluent: 25 mM Phosphate buffer pH 7
)ORZUDWHP/PLQ7HPS°C'HWHFWLRQ89QP,QMYROȝ/Sample: 1. 3\ULGR[DPLQH3\ULGR[DOPyridoxine

Application data
*UDGLHQWHOXWLRQ

L-column3 C8
1

A: CH3CN, B: 25 mM Phosphate buffer pH 7, A/B, 0/100-10/90 (0-15 min)
7
1
2

23 4
5

6

7

4

6

3

5

10
8

L-column3 C18

9Isocratic elution
25 mM Phosphate buffer pH 7
23

1

4

1

5
6

0

5

10
7LPH PLQ

415

ŶAntihistamine[Analytical conditions]
Column: ȝPSize: 4.6 mm I.D., 150 mm L.
Eluent: CH3CN/25 mM Phosphate buffer pH 7 (45/55)
Flow rate: 1 mL/min7HPS°C'HWHFWLRQ89QP,QMYROȝ/
6DPSOH&KORUSKHQLUDPLQH'LSKHQK\GUDPLQH'LSKHQ\OS\UDOLQH

+RPRFKORUF\FOL]LQH+\GUR[\]LQH&OHPDVWLQH
7. Promethazine

7

2

7

20

0

5

7
6

10
7LPH PLQŶNucleobase[Analytical conditions]
Column: L-column3 C18, ȝPSize: 4.6 mm I.D., 150 mm L.
Flow rate: 1 mL/min7HPS°C'HWHFWLRQ89QP
Inj. vol.:XSSHUȝ/ PJ/ ORZHUȝ/ PJ/6DPSOH&\WRVLQH8UDFLO&\WLGLQH*XDQLQH8ULGLQH
7K\PLQH$GHQLQH*XDQRVLQH7K\PLGLQH
10. Adenosine

15

L-colum
L-column3

Application data
Alkaline eluent

Alkaline eluent

A: 5 mM NH3 in CH3OH
B: 5 mM NH3 in H2O

A: CH3CN
B: 5 mM NH3 in H2O

Neutral eluent *Acidic eluent

A: 5 mM CH3COONH4 in CH3OH
B: 5 mM CH3COONH4 in H2O

A: CH3CN
B: 5 mM HCOOH in H2O

6LPXOWDQHRXVWHVWPHWKRGIRUSHVWLFLGHVE\
WKH/&06 DJULFXOWXUDOSURGXFWV

0

5

10

15
Time (min)

20

25

30

0

5

10

15

Time (min)Ŷ$JULFXOWXUDOFKHPLFDOVŶQuinolones[Analytical conditions]
Column: L-column3 C18, ȝPSize: 2.1 mm I.D., 150 mm L.
Eluent: A/B, 15/85-40/60-40/60-50/50-55/45-95/5-95/5(0-1-3.5-6-8-17.5-30 min)Flow rate: 0.2 mL/minTemp.: 40°CDetection: ESI-MS/MS (+)
Inj. vol.: ȝ/ ȝJ/[Analytical conditions]
Column: L-column3 C18, ȝPSize: 2.1 mm I.D., 150 mm L.
Eluent: A/B, 1/99-60/40(0-15 min)Flow rate: 0.3 mL/minTemp.: 40°CDetection: ESI-MS/MS (+)
Inj. vol.: ȝ/ ȝJ/

2
4

pH 11

Alkaline eluent
1

5

3

3

A: CH3CN/5 mM NH3 (95/5)
B: 5 mM NH3 in H2O
1

6

2
2

pH 7

4

1

5

3
6

pH 2

12

Neutral eluent *6

5* Department of Food Safety, Standards and
Evaluation Division Notification 0306 No. 4 andDepartment of Food Safety, Standards Bureau,
Inspection Division Notification 0306 No. 2

3

0

5

3

A: CH3CN/2 mM HCOONH4+50 mM HCOOH (95/5)
B: 2 mM HCOONH4+50 mM HCOOH in H2O

4

10

15

20

25

0

[Analytical conditions]
Column: L-column3 C18ȝPSize: 4.6 mm I.D., 150 mm L.
Eluent: A: CH3CN, B: P03KRVSKDWHEXIIHU
A/B, 5/95-50/50 (0-25 min)
)ORZUDWHP/PLQ7HPS°C'HWHFWLRQ89QP,QMYROȝ/ PJ/Sample: )DPRWLGLQH&LPHWLGLQH5DQLWLGLQH1L]DWLGLQH
/DIXWLGLQH5R[DWLGLQH

2

8

12

Time (min)Time (min)ŶH2EORFNHU

41

ŶOkadaic acids[Analytical conditions]
Column: L-column3 C18, ȝPSize: 2.1 mm I.D., 150 mm L.
Eluent: A/B, 60/40-60/40-100/0-100/0(0-2.5-7.5-12.5 min)Flow rate: 0.2 mL/minTemp.: 40°CDetection: ESI-MS/MS (-)
Inj. vol.: ȝ/Sample: 2NDGDLFDFLG'LQRSK\VLVWR[LQ'LQRSK\VLVWR[LQ

8L-column3

UHPLC column
UHPLC (ultra-high performance liquid chromatography) is liquid chromatography provided at a higher speed with higher separation by
XVLQJDFROXPQZLWKILQHSDUWLFOHSDFNLQJPDWHULDOVLQDSDUWLFOHVL]HRIDERXWȝPL-column3 with a wide selection range of pH values
can select analytical conditions of detection sensitivity, peak shape, and elution order based on the intended purpose

ȝP

ȝP



4
1

Analysis time: 1/2

2(OXHQWHeight equivalent to a theoretical plate(Naphthalene, ȝPSize: PP,'PP/
)ORZUDWHP/PLQ,QMYROȝ/

Size: PP,'PP/
Flow rate: 1 mL/min,QMYROȝ/tR(4) N(4) 

3tR(4) N(4) 

1

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ȝP



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

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Optimum linear velocity range

4


2

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4
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‫گ‬
Flow velocity (mm/sec)



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3

2

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

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4
7LPH PLQ



82
7LPH PLQ

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the mobile phase (eluent) with height equivalent to atheoretical plate, and the change of that value is small within
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decrease of the inner diameter of the column and the length
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4

ŶStanderd test
[Analytical conditions]
Column: L-column3 C18
Eluent: CH3CN/H22  7HPS°C; 'HWHFWLRQ89岜QP
Sample: 8UDFLO%HQ]HQH7ROXHQH1DSKWKDOHQH

L-columnVHULHV ȝP 
2SWLPXPIORZUDWHUDQJHRILQQHUGLDPHWHUPPDSSUR[P/PLQ2SWLPXPIORZUDWHUDQJHRILQQHUGLDPHWHUPPDSSUR[P/PLQ- tR: Retention time (min), N : Number of theoretical plates

Acidic eluent

Alkaline eluent
P0NH3 in H2O

P0HCOOH in H2O

Acidic eluent
1

Alkaline eluent

Neutral eluent

P0HCOOH in H2O

P0CH3COONH4 in H2O

P0NH3 in H2O
3

2

3
1
4

4

2
3

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1

2
3
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3

4

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1

2
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ŶAntidepressants (SSRI)[Analytical conditions]
Column: L-column3 C18ȝP; Size: PP,'PP/
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9

2

1
2
3

2

1

1

4





4
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8



4
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ŶH2 blocker[Analytical conditions]
Column: L-column3 C18ȝP; Size: PP,'PP/
Eluent: A: CH3CN, B: Aqueous solution
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4
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8

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Metal-free column
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10

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20 MPa 30 MPa

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100
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ŶP03KRVSKDWHEXIIHUS+
3KRVSKRULFDFLG  ȝ/ HTXLYDOHQWWRP0
6RGLXPGLK\GURJHQSKRVSKDWHGLK\GUDWHJHTXLYDOHQWWRP0$GGZDWHUWRSUHSDUHDWRWDOYROXPHRIP/
ŶP03KRVSKDWHEXIIHUS+
3RWDVVLXPGLK\GURJHQSKRVSKDWHJ HTXLYDOHQWWRP0'LVRGLXPK\GURJHQSKRVSKDWHQRQDK\GUDWHJHTXLYDOHQWWRP0$GGZDWHUWRSUHSDUHDWRWDOYROXPHRIP/
ŶP03KRVSKDWHEXIIHUS+'LSRWDVVLXPK\GURJHQSKRVSKDWHJ HTXLYDOHQWWRP0
7ULSRWDVVLXPSKRVSKDWHJ HTXLYDOHQWWRP0$GGZDWHUWRSUHSDUHDWRWDOYROXPHRIP/L-column3/LQHXS
Packing materials: L-column3 C18 (USP category: L 1) (Octadecylsilanized silica gel for liquid chromatography)
LengthParticle sizeȝP

Inner diameter

1.0 mm

10 mm

20 mm

30 mm

50 mm

75 mm

813740Cat.No.

100 mm

150 mm813610813190813020250 mmPrice (JPY)
2.1 mmCat.No.

813780813770813630813140813640813170823650823490823600823330Price (JPY)
3.0 mmCat.No.
Price (JPY)ȝP

1.0 mmCat.No.

811740811610811190Price (JPY)
1.5 mmCat.No.

2.1 mmCat.No.

3.0 mm811130811780811770Cat.No.

811160811010811630811140811640811170811020811220821650821490821600821330821260821320821060821150821460821180821070821080Price (JPY)
4.6 mmCat.No.
Price (JPY)ȝP

1.0 mmCat.No.

812740812610812130812160812010812140812170812020812220822330822260822320822040822310822070822080842510842100150 mm

250 mmPrice (JPY)
1.5 mmCat.No.

2.1 mmCat.No.

3.0 mmCat.No.

812630Price (JPY)
4.0 mm
4.6 mmCat.No.
Cat.No.

822060822150822180Price (JPY)
10.0 mm

Cat.No.
Price (JPY)- Connection type: 1/16” Waters.

Packing materials: L-column3 C8 (USP category: L 7) (Octylsilanized silica gel for liquid chromatography)
LengthParticle sizeȝP

Inner diameter

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mmCat.No.

10 mm

20 mm

30 mm

811161811011811781811771811631811141811641811171811021811221821651821491821601821331821261821321821061821151821461

821181821071821081812131812161812011812141812171812021812221822331822261822321822041822311822071822081

50 mm

75 mm

811131

100 mmPrice (JPY)
4.6 mmCat.No.
Price (JPY)ȝP

1.5 mmCat.No.

2.1 mmCat.No.

3.0 mmCat.No.

812631Price (JPY)
4.0 mm
4.6 mmCat.No.
Cat.No.
Price (JPY)- Connection type: 1/16” Waters.

13

822061822151822181

L-colum
L-column3/LQHXS
Guard column (cartridge type)
Packing materials

Particle size

L-column3 C18

ȝP

LengthSpecification

Cat.No.

2.0 mm

5 mm

Cartridge (3 pcs)

8523304.6 mm

10 mm

Cartridge (3 pcs)

8520502.0 mm

5 mm

Cartridge (3 pcs)

852331

4.6 mm

10 mm

Cartridge (3 pcs)

852051For 2.0 mm I.D.

(1 pc)

651332

For 4.6 mm I.D.

(1 pc)

651052

For 2.0 mm I.D.

(1 pc)

652332

For 4.6 mm I.D.

(1 pc)

652052ȝP

L-column3 C8

HolderInner diameter

Direct typeNon-direct typePrice (JPY)- Connection type: 1/16” Waters.
- Select a guard column with the same inner diameter as that of the analytical column or a smaller inner diameter than that of the analytical column.
- The direct connection type of dedicated holder can be directly connected to the analytical column. The indirect connection type requires tubing and connectors to connect the holder
and the analytical column.

Holder

Cartridge
Direct typeNon-direct type

Metal-free column
LengthPacking materials

Particle size

Inner diameter

L-column3 C18

ȝP

2.0 mmȝP

2.0 mmȝP

2.0 mmȝP

2.0 mmCat.No.

50 mm

100 mm

150 mm863140863170863020250 mm861140861170861020861220862140862170862020862220861141861171861021861221862141862171862021862221Price (JPY)Cat.No.
Price (JPY)Cat.No.
Price (JPY)

L-column3 C8

Cat.No.
Price (JPY)ȝP

2.0 mmCat.No.
Price (JPY)- Connection type: 1/16” Waters.

Pre-column filter
Specification

Cat.No.

Filter (1 pc), holder W+W (1 pc)

Connection type: Column side, Waters type; LC tubing side, Waters type653002Filter (1 pc), holder W+U (1 pc)

Connection type: Column side, Waters type; LC tubing side, UPLC® type653004Filter (5 pcs)

For replacement

653003

Price (JPY)- The precolumn filter prevents the entry of insoluble compounds and protects the analytical column..

ŶAbout connection type (joint)
The connection type (joint and thread type) of column is divided
into several types. The connection type is distinguished bydifferent tubing lengths at the end of the ferule.

Column end-fitting (W)PP

Tube fitting (Waters type, W)|2'PP

Always connect by using the same connection type for the LC
tubing and the column or by using tough connectors andPre-column filter to avoid the generation of dead volume.
The connection of L-column3 is the Waters type (unified finethread No.10-32UNF, outer diameter of tubing 1/16”).

1R81)

Tube fitting (UPLC® type, U)|2'PP

1R81)- Length figures: actual measurement value

14

L-column6HULHVReference: About L-column series
The L-column series appeared in 1990 and evolved corresponding to user needs with the concept of low adsorption, high durability,
high resolution, and high reproducibility. We earned a very favorable reputation because of our untiring efforts for performance andquality and a consistent stable supply for many years.
USP
category

Packing materials

L-column

L-column2

L-column3

Particle
Size
ȝP

Pore
Size
(nm)

Micro column Semi-micro andgeneral-purpose
columnSemi
preparation
columnInner diameter
less than 1 mm

Inner diameter 1 mm ormore and less than 10
mm

Inner diameter
10 mm or more

Metal-freecolumnL-column ODS

L1

3, 5

12

ż

ż

ż

-

L-column ODS-P

L1

5

30

ż

ż

-

-

L-column C8

L7

5

12

ż

ż

-

-

L-column2 ODS

L12, 3, 5

12

ż

ż

ż

ż

L-column2 C8

L7

3, 5

12

ż

ż

-

ż

L-column2 C6-Phenyl

L 11

3, 5

12

ż

ż

-

ż

L-column3 C18

L12, 3, 5

12

-

ż

ż

ż

L-column3 C8

L7

3, 5

12

-

ż

-

ż

Classification by inner diameter cited from JIS K 0124:2011

General commercial column

L-column series
L-column:
High temperature gas phase end capping
L-column2: Advanced end-capping

L-column3: Perfect stable end-capping

Interaction withbonded phase

Interaction withbonded phaseanalyteanalyteFurther
evolvedEnd-capping
layer
Dissolutionof silica

Interaction with Interaction withsilanol group metal impurities

End-capping layer

End-capping layer

Fully porous high purity silica

PCS silica (fully porous high purity silica)

Interaction with silanol group
Interaction with metal impurities

Bonding density: Low
Adsorptivity: High (tailing)
Durability: Low
Difference between lots: Large
Inert level of packing
materials

Eliminated to the utmost limit

Bonding density: High
Adsorptivity: Low (right and left symmetrical peak)
Durability: High
Difference between lots: Small

Low

General commercial column

High

<

L-column<

L-column2

L-column3

The silica-based reversed phase columns are created by modifying the bonded phases on the surface of the silica and end-capping
silanol groups. Although end-capping is performed using different methods depending on manufacturers, the complete elimination of
the silanol groups is difficult. Therefore, the peak of the analyte is subjected to tailing because of the interaction with the silanolgroups or metal impurities.
The L-column series eliminated the secondary interaction (interaction with silanol groups and metal impurities) of the silica basereversed phase column to the utmost limit by applying unique end-capping technology and pursued the separation mechanism of an
original column. Since the L-column ODS entered the stage in 1990, the columns have continually evolved.

15

L-columnmn6HULHV
6HU

Reference: About L-column series
The L-column series may present a different separation pattern in a simultaneous multicomponent analysis even when using the same
C18 (ODS) column because the manufacturing method for the packing materials is different.

L-column

L-column2

L-column3

Base material

Fully porous high purity silica

Fully porous high purity silica

PCS silica (Fully porous high purity silica)

End-cappingHigh temperature gas phase end-capping

Advanced end-capping

Perfect stable end-capping

pH 1 - pH 9

pH 1 - pH 12

Usable pH range * pH 2 - pH 9

Features

You can use without worry because thishas been stably supplied since 1990.

The influence of the silanol group was
eliminated to the utmost limit.
It offers an abundant lineup, such as thephenyl column, micro column, and nanocolumn with inner diameters less than 1
mm.
Metal free columns (glass lining) are alsoavailable.

When comparing the L-column and the
L-column2, retention is weaker, andstereoselectivity is a little lower.
The inert level of packing material is high,and it has low adsorptivity equivalent to thatof the L-column2.
Not only the alkaline resistance but also the
durability in an eluent with high water ratio
were dramatically improved.Metal freecolumns (PEEK lining) are also available.

Characteristic
test

Cat.No.622070
Column: C18, ȝP
Size: 4.6 mm I.D., 150 mm L.

Cat.No.722070

Cat.No.822070

323

1. Uracil
2. Caffeine
3. Phenol
4. Butylbenzene
5. o-Terphenyl
6. Amylbenzene
7. Triphenylene

321

Hydrogen bond property : 0.52
Hydrophobicity: 1.47
Stereoselectivity: 1.552
1

Hydrogen bond property : 0.46
Hydrophobicity: 1.46
Stereoselectivity: 1.58

756$GVRUSWLYLW\
665,

5

5 6

15

20

Cat.No.622070
Column: C18, ȝP
Size: 4.6 mm I.D., 150 mm L.

0

5

2

10
Time (min)15

20

Cat.No.722070

0

5

56

2

0

5

20

S(3) : 1.24

3

3

1
2

1

20

15

Cat.No.822070

3

15

10
Time (min)S(3) : 1.38

1

5

4

10
Time (min)S(3) : 1.58

0

7

4

10
Time (min)1. Paroxetine
2. Citalopram
3. Fluoxetine

Hydrogen bond property : 0.46
Hydrophobicity: 1.45
Stereoselectivity: 1.35

7

4

0

1

10
Time (min)

15

20

0

5

10
Time (min)1520

,QFDVHWKHERQGHGSKDVHLV& 2'6

The characteristic test evaluates the following values:<Hydrogen bond property> k (Caffeine) / k (Phenol)
This is the value representing the influence of the hydrogen bond of the silanol group of packing materials and the samples asrepresented by the ratio of the retention factors of caffeine with high hydrogen bond property and phenol with low hydrogen bondproperty. The larger the value of "hydrogen bond property", the hydrogen bonds are easy to occur between analyte and packing
materials.
<Hydrophobicity> k (Amylbenzene) / k (Butylbenzene)
This is the value representing the magnitude of the retention force of packing materials as represented by the ratio of the retention
factors of amyl benzene and butyl benzene. The larger the value of hydrophobicity, the higher the hydrophobicity of the packing
materials and the longer the retention time.
<Stereoselectivity> k (Triphenylene) / k (o-Terphenyl)
This is the value representing the plane recognition ability as represented by the ratio of the retention factors of triphenylene with aplane structure and o-terphenyl with a three-dimensional structure. The larger the value of stereoselectivity, the stronger the retentionof a compound with a plane structure.
- k: Retention factor, S : Symmetry factor

16L-column6HULHVReference: Line up L-column, L-column2
L-column2
LengthPacking materials

Particle size

L-column2 ODS(USP category: L 1)ȝP

Inner diameter
2.1 mmCat.No.

30 mm

50 mm

75 mm

100 mm

150 mm

713630713140713640713170713020723650723490723600723330250 mmPrice (JPY)
3.0 mmCat.No.
Price (JPY)ȝP

2.1 mmCat.No.

7116307111407116407111707110207112203.0 mmCat.No.

721650721490721600721330721260721320721150721460721180721070721080Price (JPY)
4.6 mmCat.No.
Price (JPY)ȝP

1.5 mm
2.1 mmCat.No.

712130712160712010Cat.No.

712140712170712020712220722150722180722070722080Price (JPY)
4.6 mmCat.No.
Price (JPY)

L-column2 C8(USP category: L 7)ȝP

2.1 mmCat.No.

711631711141711641711171711021711221

3.0 mmCat.No.

721651721491721601721331721261721321721151721461

721181721071721081712141712171712021712221722151722181722071722081Price (JPY)
4.6 mmCat.No.
Price (JPY)ȝP

2.1 mmCat.No.
Price (JPY)

4.6 mmCat.No.
Price (JPY)

L-column2 C6-Phenyl(USP category: L 11)ȝP

2.1 mmCat.No.

711636711146711646711176711026711226

3.0 mmCat.No.

721656721496721606721336721266721326721156721466721186721076721086712146712176712026712226722156722186722076722086

Price (JPY)
4.6 mmCat.No.
Price (JPY)ȝP

2.1 mmCat.No.
Price(JPY)

4.6 mmCat.No.
Price (JPY)- Connection type: 1/16” Waters.

L-column2 Metal-free column
LengthPacking materials
L-column2 ODS(USP category: L 1)

Particle size

Inner diameterȝP

2.0 mmȝP

2.0 mmCat.No.

50 mm

100 mm

150 mm

250 mm731140731170731020731220732140732170732020732220Price (JPY)Cat.No.
Price (JPY)- L-column2 Metal-free column is made from the combination of a glass lined stainless steel tube and polymer frits.
- Connection type: 1/16” Waters.

17

L-columnmn6HULHV
6HU

Reference: Line up L-column, L-column2
L-column
LengthPacking materials

Particle size

L-column ODS(USP category: L 1)ȝP

Inner diameter

30 mm

50 mm

75 mm

100 mm

150 mm

250 mm

2.1 mmCat.No.

6116306111406116406111706110206112203.0 mmCat.No.

621650621490621600621330621260621320621150621460621180621070621080612140612170612020612220622150622180622070622080612147612177612027612227622157622187622077622087612141612171612021612221622151622181622071622081Price (JPY)
4.6 mmCat.No.
Price (JPY)ȝP

2.1 mmCat.No.
Price (JPY)

4.6 mmCat.No.
Price (JPY)

L-column ODS-P(USP category: L 1)ȝP

2.1 mmCat.No.
Price (JPY)

4.6 mmCat.No.
Price (JPY)

L-column C8(USP category: L 7)ȝP

2.1 mmCat.No.
Price (JPY)

4.6 mmCat.No.
Price (JPY)- Connection type: 1/16” Waters.

Nano column / micro column with 0.075 mm I.D. - 0.3 mm I.D.
LengthParticle sizeȝP
50 mm

Particle sizeȝP
50 mm

150 mm

500 mm

50 mm

150 mm

711410711420711800712410712420711390711400711810712390712400711290711300712290712300711270711280712270712280150 mm

Packing materials

Inner diameter

L-column2 ODS(USP category: L 1)

0.075 mm

Cat.No.

0.1 mmCat.No.

0.2 mmCat.No.

7132907133000.3 mmCat.No.

713270713280Particle sizeȝP

Price (JPY)
L-column ODS(USP category: L 1)

0.075 mm

Cat.No.

6114106114206124106124200.1 mmCat.No.

6113906114006123906124000.2 mmCat.No.

611290611300612290612300Cat.No.

6112706112806122706122800.3 mmPrice (JPY)- Connection type: Valco

Trap column (cartridge type)
Packing materials

Particle size

Inner diameter

LengthL-column2 ODS
L-column ODSȝP

0.3 mmȝP

0.3 mmHolderSpecification

Cat.No.

5 mm

Cartridge (3 pcs)

7524505 mm

Cartridge (3 pcs)

652450(1 pc)

652452

For 0.3 mm I.D.

Price (JPY)- Connection type: 1/16” Waters.

Holder

Cartridge18

LC column catalog
L-column3
Ver. 02

Chemicals Evaluation andResearch Institute, Japan■ The product specifications are as of January 1, 2023. Please note that they may be changed without prior notice.
■ For questions about part prices or ordering, please contact the Chromatography Department of CERI or a nearby agency. Details areprovided on the page for the Chromatography Department of our website. Please use them as a reference.

https://www.cerij.or.jp
CERI Tokyo
Chromatography department

ISO
9001
Design,Development,Production
and supply of Column
for GC and HPLCe-mail chromato@ceri.jp

FAX +81-480-37-2521
1600 Shimotakano, Sugito-machi, Kitakatsushika-gun,
Saitama 345-0043, Japan©2019 CERI, Japan

2019/10; 2020/01; 2023/01