プログラム集
第20回日本蛋白質科学会年会
年会長 養王田 正文（東京農工大学）

目次

第20回日本蛋白質科学会年会 プログラム集

謝辞・・・・・・・・・・・・・・・・・・１ページ
若手奨励賞シンポジウム・・・・・・・・・２ページ
ポスター発表Protein Structure・・・・・・・・・・・ ３ページ～
Computation/Information science・・・・ ２１ページ～
Protein/Cellular functions, Imaging・・ ２９ページ～
Biophysics, Protein Folding・・・・・・ ３７ページ～
Proteomics / Protein engineering・・・・４６ページ～」

第 20 回日本蛋白質科学会年会
謝辞
本年会の WEB 開催にあたり、下記団体・企業からご協力を賜りましたこと御礼申し上げます。
第 20 回日本蛋白質科学会年会
年会長 養王田正文（東京農工大学 工学研究院生命機能科学部門）

スポンサー企業・団体
株式会社堀場製作所

株式会社セントラル科学貿易

DKSH ジャパン株式会社

東ソー株式会社

シスメックス株式会社

日本電子株式会社

オンライン展示会協賛企業・団体（50 音順）
DKSH ジャパン株式会社

東ソー株式会社

Cytiva（サイティバ）

日本電子株式会社

株式会社アントンパール・ジャパン
エスペック株式会社株式会社エムエステクノシステムズ
高エネルギー加速器研究機構・構造生物学研究センター
公益財団法人 高輝度光科学研究センター
シスメックス株式会社
シュレーディンガー株式会社
株式会社セントラル科学貿易
タイテック株式会社

日本分光株式会社
プライムテック株式会社
プロテイン・アイランド・松山
ベックマン・コールター株式会社
北海道和光純薬株式会社
株式会社堀場製作所
株式会社モルシス
リアルコンピューティング株式会社

テガサイエンス株式会社

その他協賛・団体
日本蛋白質構造データバンク

寄付
日本ジェネティクス株式会社

2020 年 6 月 30 日現在

1

若手奨励賞シンポジウム/Young Scientists Awards
Chairs：Yoshikazu Tanaka (Tohoku Univ.), Min Yao (Hokkaido Univ.)
YSA-1 Structure Of The Mitochondrial Protein Import Gate Reveals Distinct(P1-04) Preprotein Paths

Yuhei Araiso (Division of Health Sciences, Graduate School of Medical Science, Kanazawa University)
YSA-2 Cryo-EM structures capture the transport cycle of the P4-ATPase flippase(P1-05)○Masahiro Hiraizumi (The University of Tokyo / Mitsubishi Tanabe Pharma Corporation)

YSA-3 O2-activation and unidirectional proton-pump mechanisms of cytochrome(P1-06) c oxidase elucidated by X-ray structures of its catalytic intermediates○Atsuhiro Shimada (Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu
University)

YSA-4 Cryo-EM structures of calcium homeostasis modulator channels in diverse(P1-07) oligomeric assemblies○Kanae Demura (The University of Tokyo)
YSA-5 Developments and applications of generalized-ensemble methods for free(P2-02) energy analysis of protein-ligand binding○Hiraku Oshima (RIKEN Center for Biosystems Dynamics Research)

YSA-6 Escherichia coli small heat shock protein IbpA is an aggregation-sensor to(P3-04) self-regulate its own expression at translational levels○Tsukumi Miwa (School of Life Science and Technology, Tokyo Institute of Technology)

YSA-7 Mechanism of Antibody Aggregation by pH-Shift Stress(P4-08)○Hiroshi Imamura (Ritsumeikan University)2

Poster Presentation( ○ = Presenter )Protein Structure

P1-01 Disassembly and Reassembly of Ring-type Structure of Peroxiredoxin by
Manipulating Hydrophobic Interaction○ Tomoki Himiyama (National Institute of Advanced Industrial Science and Technology (AIST)),
Tsutomu Nakamura (National Institute of Advanced Industrial Science and Technology (AIST))

P1-02 C9orf72-derived PR poly-dipeptides compromise phase separation of
low-complexity protein sequences and nucleocytoplasmic transport○Eiichiro Mori (Nara Medical Univeristy)P1-03 Unguided Binding MD of Protein-Protein Complexes by PPI-ColDock○Kazuhiro Takemura (TokyoTech), Akio Kitao (TokyoTech)

P1-04 Structure Of The Mitochondrial Protein Import Gate Reveals Distinct
Preprotein Paths○Yuhei Araiso (Division of Health Sciences, Graduate School of Medical Science, Kanazawa University),
Akihisa Tsutsumi (Graduate School of Medicine, The University of Tokyo), Jian Qiu (University ofFreiburg), Kenichiro Imai (National Institute of Advanced Industrial Science and Technology (AIST)),
Takuya Shiota (Organization for Promotion of Tenure Track, University of Miyazaki), Haruka Sakaue(Faculty of Life Sciences, Kyoto Sangyo University / Institute for Protein Dynamics, Kyoto Sangyo University),

Junko Suzuki (Faculty of Life Sciences, Kyoto Sangyo University), Nikolaus Pfanner (University ofFreiburg), Masahide Kikkawa (Graduate School of Medicine, The University of Tokyo), Toshiya Endo
(Faculty of Life Sciences, Kyoto Sangyo University / Institute for Protein Dynamics, Kyoto Sangyo University)

P1-05 Cryo-EM structures capture the transport cycle of the P4-ATPase flippase○Masahiro Hiraizumi (The University of Tokyo / Mitsubishi Tanabe Pharma Corporation), Keitaro
Yamashita (The University of Tokyo), Tomohiro Nishizawa (The University of Tokyo), Osamu
Nureki (The University of Tokyo)

P1-06 O2-activation and unidirectional proton-pump mechanisms of cytochromec oxidase elucidated by X-ray structures of its catalytic intermediates○Atsuhiro Shimada (Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu
University), Yuki Etoh (Departement of Life Science, Graduate School of Life Science, University of Hyogo),
Kyoko Shinzawa-Itoh (Departement of Life Science, Graduate School of Life Science, University of3

Hyogo), Eiki Yamashita (Institute for Protein Research, Osaka University), Kazumasa Muramoto(Departement of Life Science, Graduate School of Life Science, University of Hyogo), Tomitake Tsukihara(Departement of Life Science, Graduate School of Life Science, University of Hyogo / Institute for Protein
Research, Osaka University), Shinya Yoshikawa (Departement of Life Science, Graduate School of Life
Science, University of Hyogo)

P1-07 Cryo-EM structures of calcium homeostasis modulator channels in diverse
oligomeric assemblies○ Kanae Demura (The University of Tokyo), Tsukasa Kusakizako (The University of Tokyo),
Wataru Shihoya (The University of Tokyo), Masahiro Hiraizumi (The University of Tokyo), Kengo
Nomura (Kyoto Prefectural University of Medicine), Hiroto Shimada (The University of Tokyo), Keitaro
Yamashita (The University of Tokyo), Tomohiro Nishizawa (The University of Tokyo), Akiyuki
Taruno (Kyoto Prefectural University of Medicine), Osamu Nureki (The University of Tokyo)

P1-08 The auto-inhibitory mechanism of isolated membrane Vo domain fromprokaryotic V/A-ATP synthase for preventing H+ leakage○Jun-ichi Kishikawa (Kyoto Sangyo University), Atsuko Nakanishi (Kyoto Sangyo University /Osaka University), Aya Furuta (Kyoto Sangyo University), Takayuki Kato (Osaka University), Keiichi
Namba (Osaka University), Masatada Tamakoshi (Tokyo University of Pharmacy and Life Sciences),
Kaoru Mitsuoka (Osaka University), Ken Yokoyama (Kyoto Sangyo University)

P1-09 Crystal Structures of Phosphatidylserine Decarboxylase Reveal the Basisof Phosphatidylethanolamine Biosynthesis○ Yasunori Watanabe (Graduate School of Agriculture, Ehime University), Seiya Watanabe(Graduate School of Agriculture, Ehime University / Center for Marine Environmental Studies (CMES), Ehime
University)

P1-10 Structural Study For The Interactions Between Mouse Sortilin And
Interferon α○ Honoka Watanabe (Graduate School of Science and Engineering, Ibaraki University / Frontier
Research Center for Applied Atomic Science, Ibaraki University), Miki Tanaka (Graduate School of Scienceand Engineering, Ibaraki University / Frontier Research Center for Applied Atomic Science, Ibaraki University),Toshiki Wada (Department of Immunology, Kanazawa Medical University), Masaki Unno (Graduate
School of Science and Engineering, Ibaraki University / Frontier Research Center for Applied Atomic Science,
Ibaraki University)

P1-11 Structural Study of cohesin loader○Sotaro Kikuchi (Nara Medical University)4P1-12 Grafting the PlexinB1-binding macrocyclic peptides into a small protein
uteroglobin without losing their binding ability○ Nozomi Nakamura (Institute for Protein Research, Osaka University), Yukiko Matsunaga(Institute for Protein Research, Osaka University), Nasir Kato Bashiruddin (Department of Chemistry,
School of Science, The University of Tokyo), Kyoko Matoba (Institute for Protein Research, Osaka
University), Keitaro Yamashita (RIKEN SPring-8 Center), Kunio Hirata (RIKEN SPring-8 Center),
Masaki Yamamoto (RIKEN SPring-8 Center), Hiroaki Suga (Department of Chemistry, School of
Science, The University of Tokyo), Junichi Takagi (Institute for Protein Research, Osaka University)

P1-13 A unique reaction mechanism of glycoside hydrolases revealed by a fungal
endo-β-1,2-glucanase
Nobukiyo Tanaka (Tokyo University of Science), ○ Masahiro Nakajima (Tokyo University of
Science), Shiro Komba (National Agriculture and Food Research Organization), Shinji Kamisuki(Azabu University), Hiroyuki Nakai (Niigata University), Akimasa Miyanaga (Tokyo Institute ofTechnology), Hayao Taguchi (Tokyo University of Science)

P1-14 Crystal Structure of HypX Reveals Molecular Mechanism of Carbon
Monoxide Production in [NiFe]-Hydrogenase Maturation○Norifumi Muraki (Exploratory Research Center on Life and Living Systems / Institute for Molecular
Sciences / SOKENDAI), Shigetoshi Aono (Exploratory Research Center on Life and Living Systems /Institute for Molecular Sciences / SOKENDAI)

P1-15 Structural Analysis of IgG1-FcγRIIIa Interaction○Yuki Yamaguchi (Graduate School of Engineering, Osaka University), Rina Yogo (Graduate Schoolof Pharmaceutical Science, Nagoya City University / Exploratory Research Center on Life and Living Systems,
ExCELLS), Hiroki Watanabe (Exploratory Research Center on Life and Living Systems, ExCELLS),

Hirokazu Yagi (Graduate School of Pharmaceutical Science, Nagoya City University), Tadashi Satoh(Graduate School of Pharmaceutical Science, Nagoya City University), Mahito Nakanishi (Biotechnology
Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology, AIST),

Masayoshi Onitsuka (Graduate School of Technology, Industrial and Social Sciences, Tokushima
University), Takeshi Omasa (Graduate School of Engineering, Osaka University / Graduate School of
Pharmaceutical Science, Nagoya City University), Natsumi Wakaizumi (Graduate School of Engineering,Osaka University), Mari Shimada (Graduate School of Engineering, Osaka University), Takahiro
Maruno (Graduate School of Engineering, Osaka University), Tetsuo Torisu (Graduate School ofEngineering, Osaka University), Shio Watanabe (Thermo Fisher Scientific), Daisuke Higo (Thermo
Fisher Scientific), Takayuki Uchihashi (Exploratory Research Center on Life and Living Systems,
ExCELLS / Graduate School of Science, Nagoya University), Saeko Yanaka (Graduate School of
Pharmaceutical Science, Nagoya City University / Exploratory Research Center on Life and Living Systems,
ExCELLS), Koichi Kato (Graduate School of Pharmaceutical Science, Nagoya City University / Exploratory
Research Center on Life and Living Systems, ExCELLS), Susumu Uchiyama (Graduate School ofEngineering, Osaka University / Exploratory Research Center on Life and Living Systems, ExCELLS)

5

P1-16 Simulation study of tree dimensional reconstruction of ribosome from Xray free electron laser diffraction patterns○Miki Nakano (RIKEN, R-CCS), Osamu Miyashita (RIKEN, R-CCS), Florence Tama (RIKEN,
R-CCS / Grad. Sch. Sci., Nagoya Univ. / ITbM, Nagoya Univ.)

P1-17 X-ray crystallographic analysis
Caldanaerobacter subterraneusofacetylxylan

esterase

from○Kohei Sasamoto (Natl. Inst. Adv. Ind. Sci. Technol. / Grad. School Sci. Eng. Setsunan Univ.), Yuya
Shimozawa (Natl. Inst. Adv. Ind. Sci. Technol. / Grad. School Sci. Eng. Setsunan Univ.), Tomoki
Himiyama (Natl. Inst. Adv. Ind. Sci. Technol.), Kunihiko Moriyoshi (Osaka Res. Inst. Ind. Sci. Technol.),
Takashi Ohmoto (Osaka Res. Inst. Ind. Sci. Technol.), Koichi Uegaki (Natl. Inst. Adv. Ind. Sci. Technol.
/ Kindai Univ.), Yoshiaki Nishiya (Grad. School Sci. Eng. Setsunan Univ.), Tsutomu Nakamura (Natl.
Inst. Adv. Ind. Sci. Technol)

P1-18 Crystal Structure of Natural Agonist-bound Lipid G protein-coupled
Receptor○Shintaro Maeda (Department of Anatomy and Developmental Biology, Graduate School of Medicine,
Kyoto University / Department of Drug Discovery Medicine, Graduate School of Medicine, Kyoto University),

Yuki Shiimura (Department of Cell Biology, Graduate School of Medicine, Kyoto University / Institute of Life
Science, Kurume University), Hidetsugu Asada (Department of Cell Biology, Graduate School of Medicine,
Kyoto University), Kunio Hirata (RIKEN SPring-8 Center), Fangjia Luo (RIKEN SPring-8 Center), Eriko
Nango (Department of Cell Biology, Graduate School of Medicine, Kyoto University / RIKEN SPring-8 Center),
Masayasu Toyomoto (Department of Anatomy and Developmental Biology, Graduate School of Medicine,
Kyoto University), So Iwata (Department of Cell Biology, Graduate School of Medicine, Kyoto University /RIKEN SPring-8 Center), Masatoshi Hagiwara (Department of Anatomy and Developmental Biology,
Graduate School of Medicine, Kyoto University)P1-19 The Structural And Mutational Analysis Of The Inhibitor-binding Pit In The
Multidrug Efflux Transporter MexB
○Naoki Koga (Graduate School of Pharmaceutical Sciences, Osaka University / Institute of Scientific andIndustrial Research, Osaka University), Seiji Yamasaki (Graduate School of Pharmaceutical Sciences,
Osaka University / Institute of Scientific and Industrial Research, Osaka University), Keisuke Sakurai(Institute of Scientific and Industrial Research, Osaka University), Ryosuke Nakashima (Institute of
Scientific and Industrial Research, Osaka University), Akihito Yamaguchi (Institute of Scientific andIndustrial Research, Osaka University), Kunihiko Nishino (Graduate School of Pharmaceutical Sciences,
Osaka University / Institute of Scientific and Industrial Research, Osaka University)6P1-20 Biochemical and structural analyses of serine proteinase involved inbiosynthesis of active-site subunit of quinohemoprotein amine
dehydrogenase○Toshinori Oozeki (Institute of Scientific and Industrial Research, Osaka University), Tadashi Nakai(Department of Food Sciences and Biotechnology, Faculty of Life Sciences, Hiroshima Institute of Technology),

Katsuyuki Tanizawa (Institute of Scientific and Industrial Research, Osaka University), Toshihide
Okajima (Institute of Scientific and Industrial Research, Osaka University)

P1-21 Study on the Encapsulation Mechanism of Guest Proteins into Bacterial
Nanocompartment○ Keiichi Noguchi (Tokyo University of Agriculture and Technology), Natsumi Sakurai (Tokyo
University of Agriculture and Technology), Motoko Fujii (Tokyo University of Agriculture and Technology),
Kosuke Kanamaru (Tokyo University of Agriculture and Technology), Akio Tamura (Tokyo Universityof Agriculture and Technology), Yosuke Fukutani (Tokyo University of Agriculture and Technology),
Hirotoshi Matsumura (Akita University), Masafumi Odaka (Akita University), Masafumi Yohda(Tokyo University of Agriculture and Technology)

P1-22 The Molecular Mechanism of tRNA Thiolation Involved in Iron-Sulfur
Enzymes and Ubiquitin-like Sulfur Donor Proteins○Masato Ishizaka (Graduate School of Life Science, Hokkaido University), Minghao Chen (Facultyof Advanced Life Science, Hokkaido University), Shun Narai (Graduate School of Life Science, Hokkaido
University), Masaki Horitani (Faculty of Agriculture, Department of Applied Biochemistry and Food Science,
Saga University), Yoshikazu Tanaka (Faculty of Advanced Life Science, Hokkaido University / Graduate
School of Life Sciences, Tohoku University), Jian Yu (Graduate School of Life Science, Hokkaido University/ Faculty of Advanced Life Science, Hokkaido University), Min Yao (Graduate School of Life Science,
Hokkaido University / Faculty of Advanced Life Science, Hokkaido University)P1-23 Reconstitution And Structural Characterization Of Di-nucleosome With
Fully Methylated DNA○Hiroshi Ushijima (Yokohama City University), Takashi Oda (Yokohama City University), Takashi
Umehara (RIKEN Center for Biosystems Dynamic Research), Tsuyoshi Konuma (Yokohama City
University), Satoko Akashi (Yokohama City University)

P1-24 X-ray Structural Study on Polyketide Synthase Involved in Olivetolic Acid
Biosynthesis in Cannabis sativa○Fumiaki Kono (National Institutes for Quantum and Radiological Science and Technology / Kyushu
University), Chiho Taguchi (Kyushu University), Michael Blaber (Florida State University, UnitedStates), Ryota Kuroki (Japan Atomic Energy Agency), Satoshi Morimoto (Kyushu University), Taro
Tamada (National Institutes for Quantum and Radiological Science and Technology)7

P1-25 Structural Basis For Cofactor Recognition Mechanism By SMAD2/3 In TGFβ Signaling○ Kenichi Miyazono (The University of Tokyo), Tomoko Ito (The University of Tokyo), Saho
Moriwaki (The University of Tokyo), Yosuke Ohno (The University of Tokyo), Hikaru Wada (The
University of Tokyo), Yui Fukatsu (Nara Institute of Science and Technology), Akira Kurisaki (NaraInstitute of Science and Technology / National Institute of Advanced Industrial Science and Technology),

Makoto Asashima (National Institute of Advanced Industrial Science and Technology), Masaru
Tanokura (The University of Tokyo)P1-26 The Interaction Analysis of Iron Chaperone PCBP1 and Human Ferritin H
and L Chain○Taro Masuda (Kyoto University), Sarju Patel (NIDDK, NIH), Olga Protchenko (NIDDK, NIH),
Caroline Philpott (NIDDK, NIH)

P1-27 Structural Elucidation Of The Reaction Mechanism Of IgG-Cleaving
Protease IdeS
○Kimika Yonekawa (Institute of Protein Research, Osaka University), Tomoaki Koyama (Instituteof Protein Research, Osaka University), Takao Arimori (Institute of Protein Research, Osaka University),

Junichi Takagi (Institute of Protein Research, Osaka University)

P1-28 Molecular basis for specific interaction between the PH domain of thehuman ceramide transfer protein CERT and the Chlamydia trachomatis
inclusion membrane protein IncD
○Toshihiko Sugiki (Osaka University), Keigo Kumagai (National Institute for Infectious Diseases),
Shoko Shinya (Osaka University), Naohiro Kobayashi (Osaka University), Toshimichi Fujiwara(Osaka University), Kentaro Hanada (National Institute for Infectious Diseases), Chojiro Kojima(Osaka University / Yokohama National University)

P1-29 Comparison of Galectin-1 Wild Type Dimer and Artificially Connected
Tandem-Repeat Mutant○Yasuhiro Nonaka (Kagawa University), Takashi Ogawa (Kagawa University), Hiroki Shoji(Kanazawa Medical University), Nozomu Nishi (Kagawa University), Shigehiro Kamitori (Kagawa
University), Takanori Nakamura (Kagawa University)

P1-30 Crystallographic studies of the cell-division protein FtsZ from Klebsiellapneumoniae and Escherichia coli
Takuya Yoshizawa (Ritsumeikan University), Junso Fujita (Osaka University), Haruna Terakado(Ritsumeikan University), ○Mayuki Ozawa (Ritsumeikan University), Natsuko Kuroda (Ritsumeikan
University), Shun-ichi Tanaka (Ritsumeikan University), Ryo Uehara (Ritsumeikan University),
Hiroyoshi Matsumura (Ritsumeikan University)8

P1-31 Crystal structure of a thermostable mutant of neuroserpin useful for drug
design○Sachiko Shinada (Osaka Prefecture University), Akihiro Harada (Osaka Prefecture University),
Rinano Suyama (Osaka Prefecture University), Takumi Yamamoto (Osaka Prefecture University),
Ryo Watatani (Osaka Prefecture University), Maki Onda (Osaka Prefecture University)

P1-32 Solution NMR structure of LaIT2, an antimicrobial peptide of Yaeyama
scorpion (Liocheles australasiae)○Maiki Tamura (Japan advanced institute of science and technology), Masahiro Miyashita (Kyoto
University), Hisashi Miyagawa (Kyoto University), Hayato Morita (Josai University), Shinya Ohki
(Japan advanced institute of science and technology)

P1-33 Structural Mechanism of Translational Repression by PABP-interacting
Protein 2○Takeru Sagae (Keio University), Mariko Yokogawa (Keio University), Ryoichi Sawazaki (Keio
University), Nao Hosoda (Nagoya City University), Shin-ichi Hoshino (Nagoya City University),
Masanori Osawa (Keio University)

P1-34 Structural analyses of ERK2 kinase complexed with allosteric inhibitors○Mayu Yoshida (Osaka Prefecture University), Takayoshi Kinoshita (Osaka Prefecture University)

P1-35 Determination of structures of chignolin and its mutant by NMR analysis○Shumpei Koroku (Meiji University), Yutaka Maruyama (RIKEN), Koh Takeuchi (AIST), Ayori
Mitsutake (Meiji University)

P1-36 Structure of the Hook-filament Junction with the FliD Cap○Atsushi Ikeda (Grad. Sch. of Sci., Osaka Univ.), Tomoko Miyata (Grad. Sch. of Frontier Biosci.,
Osaka Univ.), Fumiaki Makino (Grad. Sch. of Frontier Biosci., Osaka Univ.), Keiichi Namba (Grad.
Sch. of Frontier Biosci., Osaka Univ. / BDR, RIKEN), Katsumi Imada (Grad. Sch. of Sci., Osaka Univ.)

P1-37 Structural Analysis of FtsZ in Complex with a Fluorescent Probe BOFP
Designed from Binding Structure of Drug
Junso Fujita (Osaka University), Takuya Yoshizawa (Ritsumeikan University), Ryo Uehara(Ritsumeikan University), Mayuki Ozawa (Ritsumeikan University), ○Natsuko Kuroda (Ritsumeikan
University), Haruna Terakado (Osaka University), Daniel S. Pilch (Rutgers Robert Wood Johnson
Medical School), Hiroyoshi Matsumura (Ritsumeikan University)9P1-38 Molecular mechanism for multi-monoubiquitination of histone H3 by
UHRF1○Shun Matsuzawa (Grad. Sch. of Med Life Sci., Yokohama City Univ.), Makoto Wakatsuki (Grad.
Sch. of Med Life Sci., Yokohama City Univ.), Satomi Kori (Grad. Sch. of Med Life Sci., Yokohama City Univ.),
Ayako Furukawa (Grad. Sch. of Med Life Sci., Yokohama City Univ.), Yoshifumi Nishimura (Grad.
Sch. of Med Life Sci., Yokohama City Univ.), Atsuya Nishiyama (Inst. of Med Sci., Univ of Tokyo), Makoto
Nakanishi (Inst. of Med Sci., Univ of Tokyo), Kyohei Arita (Grad. Sch. of Med Life Sci., Yokohama City
Univ.)

P1-39 Changes in the structure and function of the vertebrate p53 family proteinsin evolution process○Shuya Sakaguchi (Laboratory of Biological Chemistry, Graduate School of Chemical Sciences andEngineering, Hokkaido University), Natsumi Nakagawa (Laboratory of Biological Chemistry, Graduate
School of Chemical Sciences and Engineering, Hokkaido University), Haytham Wahba (Department ofBiochemistry and Molecular Medicine, University of Montreal, Canada), Rui Kamada (Laboratory of
Biological Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University), James G.
Omichinski (Department of Biochemistry and Molecular Medicine, University of Montreal, Canada),
Kazuyasu Sakaguchi (Laboratory of Biological Chemistry, Department of Chemistry, Faculty of Science,
Hokkaido University)

P1-40 Evaluation Of Oligomer States
Glycoprotein For Structure Studies

Of

Recombinant

HIV-2

Envelope○Yuki Anraku (Faculty of Pharm sci., Hokkaido University / Grad. Sch. Life sci., Hokkaido University),
Shunsuke kita (Faculty of Pharm sci., Hokkaido University / Grad. Sch. Life sci., Hokkaido University),
Hideo Fukuhara (Faculty of Pharm sci., Hokkaido University / Grad. Sch. Life sci., Hokkaido University),
Takaki Akiyama (Faculty of Pharm sci., Hokkaido University), Simon Davis (University of Oxford, UK),
Atsushi Furukawa (Faculty of Pharm sci., Hokkaido University / Grad. Sch. Life sci., Hokkaido University ),Thushan de Silva (University of Oxford, UK), James E. Robinson (University of Tulane, US),
Yuguang Zhao (University of Oxford, UK), Yvonne Jones (University of Oxford, UK), David Stuart(University of Oxford, UK), Juha T. Huiskonen (University of Oxford, UK), Sarah Rowland-Jones(University of Oxford, UK), Katsumi Maenaka (Faculty of Pharm sci., Hokkaido University / Grad. Sch.
Life sci., Hokkaido University)

P1-41 Structural Basis for the Hepatocyte-specific Entry of Hepatitis B Virus○Tomoaki Ishiba (Keio University Faculty of Pharmacy), Mariko Yokogawa (Keio University Facultyof Pharmacy), Terumi Yokota (Keio University Faculty of Pharmacy), Taiki Muroi (Keio University Facultyof Pharmacy), Masanori Osawa (Keio University Faculty of Pharmacy)10

P1-42 Domain Structural Analysis For The Insecticidal And Antibacterial TwoDomain Toxin,LaIT2,From Japanese Scorpion, Liocheles australasiae○Chiharu Tatsushiro (Josai University), Hayato Morita (Josai University)

P1-43 Observation of Structural Change Of α-Synuclein, a Intrinsically Disordered
Protein by Fluorescence Lifetime Measurement○Ko Sasada (Graduate School of Science, Kobe University), Koichi Fujii (Graduate School of Science,
Kobe University), Ryosuke Matsubara (Graduate School of Science, Kobe University), Tetsunari
Kimura (Graduate School of Science, Kobe University)

P1-44 Structural basis for the switching of binding partners by Ser298
phosphorylation in UHRF1○Satomi Kori (Yokohama City University), Tomohiro Jimenji (Yokohama City University), Toru
Ekimoto (Yokohama City University), Miwa Sato (Mitsui Knowledge Industry), Fumie Kusano(Yokohama City University), Takashi Oda (Yokohama City University), Motoko Unoki (Kyushu
University), Mitsunori Ikeguchi (Yokohama City University), Kyohei Arita (Yokohama City University)

P1-45 A mutation to give an antibody a peptidase activity○Jun Kobayashi (SBRC, IMSS, KEK), Emi Hifumi (Research Promo. Inst., Oita Univ.), Taizo Uda(Nanotech Lab. ISIT), Ryuichi Kato (SBRC, IMSS, KEK)

P1-46 Cryo-EM Analysis of Nucleosomal Transcription○Haruhiko Ehara (RIKEN), Tomoya Kujirai (RIKEN / The University of Tokyo), Yuka Fujino (The
University of Tokyo / Waseda University), Mikako Shirouzu (RIKEN), Hitoshi Kurumizaka (RIKEN /The University of Tokyo / Waseda University), Shun-ichi Sekine (RIKEN)

P1-47 Neutron diffraction studies of [NiFe]-hydrogenase from Desulfovibrio
vulgaris Miyazaki F○Taro Tamada (National Institutes for Quantum and Radiological Science and Technology), Takeshi
Hiromoto (National Institutes for Quantum and Radiological Science and Technology / University of Hyogo),
Koji Nishikawa (University of Hyogo), Yu Hirano (National Institutes for Quantum and Radiological
Science and Technology), Katsuhiro Kusaka (Ibaraki University), Leighton Coates (Oak Ridge
National Laboratory), Yoshiki Higuchi (University of Hyogo)

P1-48 Recent Progress Of BioSAXS Equipment For Complex Molecular Systems
At The Photon Factory○Kento Yonezawa (High Energy Accelerator Research Organization), Masatsuyo Takahashi (HighKeiko Yatabe (High Energy Accelerator Research
Organization), Yasuko Nagatani (High Energy Accelerator Research Organization), Yugo Hayashi(Nara Institute of Science and Technology), Shinji Amano (Nara Institute of Science and Technology),

Energy Accelerator Research Organization),11

Hironari Kamikubo (High Energy Accelerator Research Organization / Nara Institute of Science andTechnology), Nobutaka Shimizu (High Energy Accelerator Research Organization)

P1-49 The Structural Change Mechanism In The Catalytic Cycle Of Malate
Dehydrogenase○ Yuya Shimozawa (Setsunan University / National Institute of Advanced Industrial Science andTechnology), Yoshiaki Nishiya (Setsunan University), Kohei Sasamoto (Setsunan University /National Institute of Advanced Industrial Science and Technology), Tomoki Himiyama (National Instituteof Advanced Industrial Science and Technology), Tsutomu Nakamura (National Institute of Advanced
Industrial Science and Technology)

P1-50 Conformational changes of influenza virus hemagglutinin under acidic
condition○Takaki Akiyama (Laboratory of Biomolecular Science, Faculty of Pharmaceutical Science, Hokkaido
University), Hideo Fukuhara (Center for Research and Education on Drug Discovery, Faculty of
Pharmaceutical Science, Hokkaido University), Mihiro Kawamura (Laboratory of Biomolecular Science,
Faculty of Pharmaceutical Science, Hokkaido University), Katsumi Maenaka (Laboratory of Biomolecular
Science, Faculty of Pharmaceutical Science, Hokkaido University / Center for Research and Education on Drug
Discovery, Faculty of Pharmaceutical Science, Hokkaido University)

P1-51 Thioflavin T Fluorescence Lifetime Assay Of Homogeneous Insulin Amyloid
Fibrils○ Akinori Oda (Hamamatsu Photonics K.K.), Tomoo Inubushi (Hamamatsu Photonics K.K.),
Keisuke Yuzu (Grad. Sch. Sci., Kobe Univ.), Eri Chatani (Grad. Sch. Sci., Kobe Univ.), Etsuji
Yoshikawa (Hamamatsu Photonics K.K.), Hiroshi Satozono (Hamamatsu Photonics K.K.)

P1-52 Mapping Analysis to Probe The Local/Global Conformational Changes inProteins by FTIR Spectroscopy with Aspartic Acid Scanning○Masanori Hashimoto (Nagoya Institute of Technology), Kota Katayama (Nagoya Institute ofTechnology), Manish Singh (Nagoya Institute of Technology), Yuji Furutani (Nagoya Institute ofTechnology), Hideki Kandori (Nagoya Institute of Technology)

P1-53 Crystal structure analysis of BIR3 domain of ubiquitin ligase in complex
with its specific ligand○ Makoto Okabe (Graduate School of Life Sciences, Tohoku University), Takeshi Yokoyama(Graduate School of Life Sciences, Tohoku University), Shusuke Tomoshige (Graduate School of Life
Sciences, Tohoku University), Tomohisa Ogawa (Graduate School of Life Sciences, Tohoku University),
Minoru Ishikawa (Graduate School of Life Sciences, Tohoku University), Yoshikazu Tanaka(Graduate School of Life Sciences, Tohoku University)12P1-54 Structure Change of MotS from Bacillus subtilis by Sodium Ion○Ayaka Yamaguchi (Grad. Sch. of Sci., Osaka Univ.), Norihiro Takekawa (Grad. Sch. of Sci.,
Osaka Univ.), Miki Kinoshita ( Grad. Sch. of Frontier Biosci., Osaka Univ.), Tohru Minamino ( Grad.
Sch. of Frontier Biosci., Osaka Univ.), Katsumi Imada (Grad. Sch. of Sci., Osaka Univ.)

P1-55 Single particle analysis of silkworm storage proteins○Shunsuke Kita (Faculty of Pharmaceutical Sciences, Hokkaido University), Katsumi Maenaka(Faculty of Pharmaceutical Sciences, Hokkaido University)

P1-56 Structure and comparison of two anti-cancer lectins from marine mussels○Kenichi Kamata (Yokohama City University), Kenji Mizutani (Yokohama City University), Katsuya
Takahashi (Yokohama City University), Tsuyoshi Konuma (Yokohama City University), Takahisa
Ikegami (Yokohama City University), Yuki Fujii (Nagasaki International University), Yasuhiro Ozeki(Yokohama City University), Jeremy R.H. Tame (Yokohama City University)

P1-57 Impacts Of The Introduction Of Hydrophobic Residues On The Protein
Crystallization○Megumi Kosaka (Okayama University), Hidenori Yamada (Okayama University), Junichiro
Futami (Okayama University), Hiroko Tada (Okayama University), Koreyoshi Imamura (Okayama
University), Taro Tamada (QST)

P1-58 Crystal structure of dog allergen Can f 1○Keisuke Suda (Graduate School of Life and Environmental Sciences, Osaka Prefecture University),
Haruka Muroya (Graduate School of Life and Environmental Sciences, Osaka Prefecture University),
Yuma Fukutomi (Clinical Research Center for Allergy and Rheumatology, National Hospital Organization
Sagamihara National Hospital), Shigenori Nishimura (Graduate School of Life and Environmental
Sciences, Osaka Prefecture University), Osamu Ishibashi (Graduate School of Life and Environmental
Sciences, Osaka Prefecture University), Takashi Inui (Graduate School of Life and Environmental Sciences,
Osaka Prefecture University)

P1-59 The tail tube structure of the jumbo Staphylococcus phage S6 by cryo-EM○Wataru Koibuchi (University of Tsukuba), Jumpei Uchiyama (Azabu University), Shigenobu
Matsuzaki (Kochi University), Kazuyoshi Murata (National Institute for Physiological Sciences), Kenji
Iwasaki (University of Tsukuba), Naoyuki Miyazaki (University of Tsukuba)

P1-60 Capsid stabilization mechanisms of Helicobacter pylori bacteriophage
KHP30○Ryosuke Kamiya (University of Tsukuba), Jumpei Uchiyama (Azabu University), Shigenobu
Matsuzaki (Kochi University), Kazuyoshi Murata (National Institute for Physiological Sciences), Kenji
Iwasaki (University of Tsukuba), Naoyuki Miyazaki (University of Tsukuba)

13

P1-61 The Catalytic Mechanism of a PPIase Chaperone Investigated by the
Structural Analysis at the Transition State Using NMR and MD Simulation○Soichiro Kawagoe (Graduate School of Chemical Sciences and Engineering, Hokkaido University),
Tomohide Saio (Graduate School of Chemical Sciences and Engineering, Hokkaido University /Department of Chemistry, Faculty of Science, Hokkaido University), Hiroshi Nakagawa (Materials
Sciences Research Center Atomic Energy Agency), Hiroyuki Kumeta (Faculty of Advanced Life Science,
Hokkaido University), Koichiro Ishimori (Graduate School of Chemical Sciences and Engineering,
Hokkaido University / Department of Chemistry, Faculty of Science, Hokkaido University)

P1-62 Bactericidal Mechanisms Of An α-Defensin, Cryptdin-4: Effect Of Peptide
Redox And Culture Condition Of E. coli
○Yi Wang (Graduate School of Life Science, Hokkaido University), Rina Hiramine (Graduate Schoolof Life Science, Hokkaido University), Chisato Toyokawa (Graduate School of Life Science, Hokkaido
University), Tomoyasu Aizawa (Graduate School of Life Science, Hokkaido University / Global Institution
for Collaborative Research and Education, Hokkaido University)

P1-63 Development of Expression of Recombinant Small Peptides ThroughCalmodulin-fusion Protein System○Hao Gu (Graduate School of Life Science, Hokkaido University), Koki Onuma (Graduate School ofLife Science, Hokkaido University), Hiroaki Ishida (Department of Biological Sciences, University ofCalgary), Yasuhiro Kumaki (Graduate School of Life Science, Hokkaido University), Takashi
Tsukamoto (Graduate School of Life Science, Hokkaido University / Global Institution for Collaborative
Research and Education, Hokkaido University), Takashi Kikukawa (Graduate School of Life Science,
Hokkaido University / Global Institution for Collaborative Research and Education, Hokkaido University),

Makoto Demura (Graduate School of Life Science, Hokkaido University / Global Institution for Collaborative
Research and Education, Hokkaido University), Hans J. Vogel (Department of Biological Sciences,
University of Calgary), Tomoyasu Aizawa (Graduate School of Life Science, Hokkaido University / Global
Institution for Collaborative Research and Education, Hokkaido University)

P1-64 Determining the domain arrangement of bacterial site-2 protease by thesite-specific insertion of PA tag and labeling with NZ-1 Fab○Rie Aruga (Graduate school of Medical Life Science, Yokohama City University), Risako Tamura(Graduate school of Medical Life Science, Yokohama City University), Mika Hirose (Institute for Protein
Research, Osaka University), Rika Oi (Graduate school of Medical Life Science, Yokohama City University),
Mika K. Kaneko (Tohoku University Graduate School of Medicine), Yukinari Kato (Tohoku University
Graduate School of Medicine), Kenji Iwasaki (Life Science Center for Survival Dynamics, Tsukuba
Advanced Research Alliance, University of Tsukuba), Terukazu Nogi (Graduate school of Medical Life
Science, Yokohama City University)14

P1-65 Gene Identification Of An Allergen Peptide From Cypress Pollen Using Next
Generation Sequencer And Its Over Expression For Analysis○ Tomona Iizuka (Graduate School of Life Science, Hokkaido University), Hélène Sénéchal
( Biochemistry Department, Armand Trousseau Children Hospital / Immunology Department, Institute Pasteur),

Pascal Poncet ( Biochemistry Department, Armand Trousseau Children Hospital / Immunology Department,
Institute Pasteur), Tomoyasu Aizawa (Graduate School of Life Science, Hokkaido University / Global
Institution for Collaborative Research and Education, Hokkaido University)

P1-66 Analysis Of Substrate Recognition For Ser/Thr Phosphatase PPM1A○Itsumi Tani (Laboratory of Biological Chemistry, Department of Chemistry, Faculty of Science, Hokkaido
University), Yukiko Shirahata (Laboratory of Biological Chemistry, Department of Chemistry, Faculty of
Science, Hokkaido University), Kei Kawamura (Laboratory of Biological Chemistry, Department ofChemistry, Faculty of Science, Hokkaido University), Shogo Ito (Laboratory of Biological Chemistry,
Department of Chemistry, Faculty of Science, Hokkaido University), Rui Kamada (Laboratory of Biological
Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University), Kazuyasu Sakaguchi(Laboratory of Biological Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University)

P1-67 Enzymatic Activity of RNase H2 Toward Oxidized
Ribonucleoside Monophophates Embedded in DNA

andAbasic○ Yuki Ishizuka (Ritsumeikan University), Ryo Uehara (Ritsumeikan University), Takuya
Yoshizawa (Ritsumeikan University), Hiroyuki Matsumura (Ritsumeikan University)P1-68 Interaction Mechanism between α1-Acid Glycoprotein and Membrane
Characterized by Vacuum-Ultraviolet Circular-Dichroism Spectroscopy○ Koichi Matsuo (Hiroshima Synchrotron Radiation Center, Hiroshima University), Munehiro
Kumashiro (Department of Physical Science, Graduate School of Science, Hiroshima University),
Kunihiko Gekko (Hiroshima Synchrotron Radiation Center, Hiroshima University)

P1-69 High-resolution structure analysis of NADH-cytochrome b5 reductase○Yu Hirano (Institute for Quantum Life Science, National Institutes for Quantum and Radiological Scienceand Technology / PRESTO, Japan Science and Technology Agency), Kazuo Kurihara (Institute forQuantum Life Science, National Institutes for Quantum and Radiological Science and Technology), Katsuhiro
Kusaka (Frontier Research Center for Applied Atomic Science, Ibaraki University), Andreas Ostermann(Technishe Universitat Munchen, Forshungs-Neutronenquelle Heinz Maier-Leibnitz ), Shigenobu Kimura(Graduate School of Science and Engineering, Ibaraki University), Kunio Miki (Graduate School of Science,
Kyoto University), Taro Tamada (Institute for Quantum Life Science, National Institutes for Quantum andRadiological Science and Technology)15

P1-70 Molecular Basis for the Intramolecular Regulation of DGKα: Structural andBiophysical Characterization of its N-terminal Domains○Daisuke Takahashi (Kyushu University), Fumio Sakane (Chiba University)

P1-71 Enhancement Of The Expression Level Of Recombinant α-defensin Derived
From Mouse By Inclusion Body Formation In The E. coli Cytoplasm○Yuchi Song (Graduate School of Life Science, Hokkaido University), Tomoyasu Aizawa (Graduate
School of Life Science, Hokkaido University / Global Institution for Collaborative Research and Education,
Hokkaido University)

P1-72 A new role of cellular zinc for protein quality control in the early secretory
pathway○Satoshi Watanabe (Tohoku University), Yuta Amagai (Tohoku University), Sara Sannino(Ospedale San Raffaele), Amiko Miyake (Tohoku University), Roberto Sitia (Ospedale San Raffaele),
Kenji Inaba (Tohoku University)

P1-73 Molecular Recognition Of An Innate Immune Sensor NONO Toward HIV-2
Capsid○Nanao Fujitani (Hokkaido University), Kimiko Kuroki (Hokkaido University), Cécile Conrad(Institut Curie), Xavier Lahaye (Institut Curie), Felix Rey (Institut Pasteur), Nicolas Manel (Institut
Curie), Katsumi Maenaka (Hokkaido University)

P1-74 Improved Structure Refinement in MAINMAST for de novo Modeling from
Cryo-EM Maps
○Takaharu Mori (RIKEN), Genki Terashi (Purdue University), Daisuke Matsuoka (RIKEN),
Daisuke Kihara (Purdue University), Yuji Sugita (RIKEN)

P1-75 Mechanistic insight into dysregulation of FUS liquid-liquid phaseseparation by toxic repeat peptide○Honoka Kawamukai (Graduate School of Chemical Sciences and Engineering, Hokkaido University),
Tomohide Saio (Graduate School of Chemical Sciences and Engineering, Hokkaido University / Faculty of
Science, Hokkaido University), Koichiro Ishimori (Graduate School of Chemical Sciences and Engineering,
Hokkaido University / Faculty of Science, Hokkaido University)

P1-76 Molecular basis of JAK-STAT signal pathway inhibition by rabies virus
P-protein○ Aoi Sugiyama (Hokkaido University), Miku Minami (Hokkaido University), Xinxin Jiang(Hokkaido University), Yuma Nagano (Hokkaido University), Tomo Nomai (Hokkaido University),
Takuya Wakahara (Hokkaido University), Katsumi Maenaka (Hokkaido University), Min Yao(Hokkaido University), Toyoyuki Ose (Hokkaido University)16

P1-77 Analysis Of Ligand Recognition And Activation Mechanisms In Muscarinic
Acetylcholine Receptor 2 (mACR2) By ATR-FTIR Spectroscopy○Kohei Suzuki (Nagoya Institute of Technology), Kota Katayama (Nagoya Institute of Technology),
Ryoji Suno (Kansai Medical University. Medical), Hideki Kandori (Nagoya Institute of Technology)

P1-78 Secondary-Structure Analysis of Aggregated Keratin Protein Using
Vacuum-Ultraviolet Circular Dichroism Spectroscopy○Motoki Takeda (Milbon Co., Ltd.), Atsushi Baba (Milbon Co., Ltd.), Yudai Izumi (Hiroshima
Synchrotron Radiation Center, Hiroshima University), Koichi Matsuo (Hiroshima Synchrotron Radiation
Center, Hiroshima University), Len Ito (Milbon Co., Ltd.)

P1-79 Structural Modeling Of LaIT2 As A Peptide Toxin In The Venom Of Japanese
Scorpion Liocheles australasiae, With Cross Validation Experiments Using
Polyclonal Antibodies For LaIT2 And C-LaIT2
Takuya Izawa (Josai Unviersity), Chiharu Tatsushiro (Josai Unviersity), Maiki Tamura (Japan
Advvanced Institute of Sicence and Technology), Masahiro Miyashita (Kyoto Unversity), Hayato

Eugene Morita (Kyoto Unversity), Shinya Ohki (Kyoto Unversity), ○Hayato Eugene Morita(Josai Unviersity)

P1-80 Effect Of Metal Ions On Phosphatase Activity Of Ser/Thr Phosphatase PPM
Family Members○Takaaki Ozawa (Laboratory of Biological Chemistry, Department of Chemistry, Faculty of Science,
Hokkaido University), Shogo Ito (Laboratory of Biological Chemistry, Department of Chemistry, Faculty of
Science, Hokkaido University), Ryo Iwamuro (Laboratory of Biological Chemistry, Department of Chemistry,
Faculty of Science, Hokkaido University), Rui Kamada (Laboratory of Biological Chemistry, Department ofChemistry, Faculty of Science, Hokkaido University), Kazuyasu Sakaguchi (Laboratory of Biological
Chemistry, Department of Chemistry, Faculty of Science, Hokkaido University)

P1-81 Host Specificity and Structure of Bacteriophage Receptor Binding Protein○Shuji Kanamaru (Tokyo Institute of Technology)

P1-82 Elucidation of interaction between neurotrophin receptor TrkAd5 and its
binding peptide○Maho Takahashi (Yokohama City University), Toru Ekimoto (Yokohama City University), Tsutomu
Yamane (Yokohama City University), Rika Suzuki (Yokohama City University), Hideo Takahashi(Yokohama City University), Mitsunori Ikeguchi (Yokohama City University / RIKEN)

17

P1-83 Structural Characterization Of A Novel Exo-α-D-fructofuranosidase From
Bifidobacterium dentium That Degrades Caramelized Sugars○Toma Kashima (The University of Tokyo), Kouki Okumura (Kagoshima University), Takatoshi
Arakawa (The University of Tokyo), Akihiro Ishiwata (RIKEN), Yukishige Ito (RIKEN), Kiyotaka
Fujita (Kagoshima University), Shinya Fushinobu (The University of Tokyo)P1-84 Targeting the cryptic sites: NMR-based strategy to improve the druggabilityof proteins via dynamics modulation○ Koh Takeuchi (National Institute for Advanced Industrial Science and Technology), Yumiko
Mizukoshi (JBIC), Yuji Tokunaga (National Institute for Advanced Industrial Science and Technology),
Ichio Shimada (The Univ. of Tokyo, Grad Sch Pharm Sci)

P1-85 Cryo-EM, Crystallographic And Kinetic Study Of Quinol-dependent Nitric
Oxide Reductases From Neisseria Meninigitidis○Muhamad Arif Bin Mohamad Jamali (Graduate School of Life Science, University of Hyogo),
Chai C. Gopalasingam (Molecular Biophysics Group, Institute of Integrative Biology, Faculty of Healthand Life Sciences, University of Liverpool, England), Rachel M. Johnson (School of Biomedical Sciences,
Faculty of Biological Sciences, University of Leeds, England / Astbury Centre for Structural and Molecular
Biology, University of Leeds, England), Stephen P Muench (School of Biomedical Sciences, Faculty ofBiological Sciences, University of Leeds, England / Astbury Centre for Structural and Molecular Biology,
University of Leeds, England), Svetlana V. Antonyuk (Molecular Biophysics Group, Institute of Integrative
Biology, Faculty of Health and Life Sciences, University of Liverpool, England), Samar S. Hasnain(Molecular Biophysics Group, Institute of Integrative Biology, Faculty of Health and Life Sciences, University ofLiverpool, Liverpool, England), Takehiko Tosha (RIKEN SPring-8 Center), Kazumasa Muramoto(Graduate School of Life Science, University of Hyogo), Yoshitsugu Shiro (Graduate School of Life Science,
University of Hyogo)

P1-86 Reconstitution of full-complex of bacterial heme transporter into theplatforms suitable for structural analysis○ Ayaho Abe (University of Hyogo), Hideki Shigematsu (RIKEN SPring-8 Center), Masaki
Yamamoto (RIKEN SPring-8 Center), Hiroshi Sugimoto (University of Hyogo / RIKEN SPring-8 Center)

P1-87 Crystal Structure of Enantiospecific Decarboxylase Involved in Phenyl
Coumaran Catabolism○Takumi Sawaya (National Institute of Technology, Asahikawa College), Miki Senda (Institute ofMaterials Structure Science, High Energy Accelerator Research Organization (KEK)), Takaaki Yoshida(National Institute of Technology, Asahikawa College), Kotone Asahina (National Institute of Technology,
Asahikawa College), Hiroshi Matsuura (National Institute of Technology, Asahikawa College), Naofumi
Kamimura (Nagaoka University of Technology), Eiji Masai (Nagaoka University of Technology), Toshiya
Senda (Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK)),
Keisuke Sugimoto (National Institute of Technology, Asahikawa College)18

P1-88 A Novel Analysis Method for Free-energy Landscape of Protein Domain
Motion Deduced from Cryo-EM Images○Mao Oide (Theoretical Molecular Science Laboratory, RIKEN / Department of Physics, Keio University),
Tomotaka Oroguchi (Department of Physics, Keio University), Taiki Wakabayashi (Department of
Physics, Keio University), Takayuki Kato (Protein Institute, Osaka Univerisity), Masayoshi Nakasako(Department of Physics, Keio University)P1-89 Stable metalloprotein composed of a bulky synthetic porphyrin and theheme acquisition protein secreted by Pseudomonas aeruginosa○Garyo Ueda (Nagoya University), Yuma Shisaka (Nagoya University), Erika Sakakibara(Nagoya University), Hiroshi Sugimoto (RIKEN SPring-8 Center / Core Research for Evolutional Scienceand Technology), Shoji Osami (Nagoya University / Core Research for Evolutional Science and Technology)

P1-90 Crystal structure analysis of an autoimmune disease susceptible allele
CD72c○Nobutaka Numoto (Department of Structural Biology, Medical Research Institute, Tokyo Medical and
Dental University), Takeshi Tsubata (Department of Immunology, Medical Research Institute, Tokyo
Medical and Dental University), Nobutoshi Ito (Department of Structural Biology, Medical Research Institute,Tokyo Medical and Dental University)P1-91 Structural Stability and Dynamics of NCPs Reconstituted with Diverse DNA
Sequences○Tsuyoshi Konuma (Yokohama City University), Kohei Hashimoto (Yokohama City University),
Kanako Sato (Yokohama City University), Satoko Akashi (Yokohama City University)P1-92 The residues determining CYP81A6 activity○Miki H. Maeda (National Agriculture and Food Research Organization), Shunsuke Yamamoto(Kumiai Chemical Industries, Co., LTD.), Yoshitaka Tanetani (Kumiai Chemical Industries, Co., LTD.),
Kiyoshi Kawai (Kumiai Chemical Industries, Co., LTD.), Rintaro Suzuki (National Agriculture and Food
Research Organization)

P1-93 SAXS Analysis of the Magnetorecepter Candidate Protein ISCA1○ Shigeki Arai (National Institutes for Quantum and Radiological Science and Technology), Rumi
Shimizu (National Institutes for Quantum and Radiological Science and Technology), Motoyasu Adachi(National Institutes for Quantum and Radiological Science and Technology), Satoshi Ajito (Gunma Univ.),
Mitsuhiro Hirai (Gunma Univ.)

19

P1-94 Elucidation of the structural change in heat activation of the alcohol
dehydrogenase from Sulfolobus tokodaii○Shuichiro Goda (Soka University / Nagasaki University), Sho Takashima (Nagasaki University),
Kosei Kajiyama (Nagasaki University), Yuka Nagano (Nagasaki University), Takuro Uchida(Nagasaki University), Hideaki Unno (Nagasaki University), Tomomitsu Hatakeyama (Nagasaki
University)P1-95 Structural Basis for Quenchbody (Q-body): Crystal Structures of the Fab
Fragment of Anti-osteocalcin Antibody KTM219○Shuma Yazaki (Shinshu University), Misaki Komatsu (Shinshu University), Jinhua Dong (Tokyo
Institute of Technology), Hiroshi Ueda (Tokyo Institute of Technology), Ryoichi Arai (Shinshu University)

P1-96 Basic Properties of Newly Developed MALS Detector and its Application toMAb○ Hiroshi Tomizawa (Tosoh corporation), Jukka Kervinen
Soleymnnazhad (Tosoh Biosccience LLC.)(Tosoh Biosccience LLC),

Ali

20

Computation/Information scienceP2-01 Simulating the association/dissociation process of flexible protein
complexes○Tran P. Duy (Tokyo Institute of Technology), Akio Kitao (Tokyo Institute of Technology)

P2-02 Developments and applications of generalized-ensemble methods for freeenergy analysis of protein-ligand binding○Hiraku Oshima (RIKEN Center for Biosystems Dynamics Research)

P2-03 Elucidation of local structure contributing to protein stability: Structuralfeatures of loop residues of 4-strand β-sheet motif○Megumi Nakajima (Nagoya University), George Chikenji (Nagoya University)P2-04 Structure prediction of the transmembrane domain of alpha- and betasecretases and the structural differences○Kaori Yanagino (Kindai University), Naoyuki Miyashita (Kindai University)

P2-05 Conformational Changes of Heme ABC Transporter BhuUV-T Revealed by
Molecular Simulations○Koichi Tamura (RIKEN R-CCS), Yuji Sugita (RIKEN R-CCS / RIKEN CPR / RIKEN BDR)

P2-06 Molecular Dynamics Simulation of
the drug binding

Monoamine Oxidase B (MAO-B) and○Masaki Ottawa (BOST Kindai University), Lisa Matsukura (BOST Kindai University), Kaori
Yanagino (BOST Kindai University), Naoyuki Miyashita (BOST Kindai University), Ryuichi Harada(School of Medicine, Tohoku University), Yuichi Kimura (BOST Kindai University), Syozo Furumoto(CYRIC Tohoku University)P2-07 Mechanism of DNA Transcription Bubble in Transcription Initiation
Complex Studied by Molecular Simulation○Genki Shino (Department of Biophysics, Division of Biological Sciences, Graduate School of Science,
Kyoto University), Masahiro Shimizu (Department of Biophysics, Division of Biological Sciences, Graduate
School of Science, Kyoto University), Shintaroh Kubo (Department of Biophysics, Division of Biological
Sciences, Graduate School of Science, Kyoto University), Toru Niina (Department of Biophysics, Division of
Biological Sciences, Graduate School of Science, Kyoto University), Shoji Takada (Department ofBiophysics, Division of Biological Sciences, Graduate School of Science, Kyoto University)21

P2-08 Molecular Modeling and Interaction Analysis of C3a Anaphylatoxin
Chemotactic Receptor (C3aR1)○Kensuke Misawa (Kao Corporation), Yoshiya Sugai (Kao Corporation), Takatsugu Hirokawa(National Institute of Advanced Industrial Science and Technology / University of Tsukuba)

P2-09 Molecular Dynamics Study on Functional Regulation of Transcription
Factors by Phosphorylation on the Intrinsically Disordered Region○Kota Kasahara (College of Life Sciences, Ritsumeikan University), Toru Sengoku (Graduate Schoolof Medicine, Yokohama City University), Junichi Higo (Graduate School of Simulation Studies, University of
Hyogo), Takuya Takahashi (College of Life Sciences, Ritsumeikan University), Kazuhiro Ogata(Graduate School of Medicine, Yokohama City University), Haruki Nakamura (Institute for Protein
Research, Osaka University)

P2-10 Influences of cholesterol on dimerization of transmembrane peptides witha GxxxG motif studied by molecular dynamics simulations○Hayato Itaya (Graduate School of Life Sciences, Ritsumeikan University), Kota Kasahara (Collegeof Life Sciences, Ritsumeikan University), Yosiaki Yano (Graduate School and Faculty of Pharmaceutical
Sciences, Kyoto University), Katsumi Matsuzaki (Graduate School and Faculty of Pharmaceutical
Sciences, Kyoto University), Takuya Takahashi (College of Life Sciences, Ritsumeikan University)P2-11 Molecular Dynamics Simulation of HSP90 Dimer andof the Peptide Drug

Docking Simulation○Lisa Matsukura (KINDAI University), Naoyuki Miyashita (KINDAI University / University of ElectroCommunications), Masumi Taki (University of Electro-Communications), Shinichi Watanabe(University of Electro-Communications)P2-12 Energy analysis of folding simulations of NuG2
Yutaka Maruyama (RIKEN), ○Ayori Mitsutake (Meiji University)P2-13 Development of de novo Method for Searching Protein-Ligands Binding
Pathway: Ligand-Docking Parallel Cascade Selection Molecular Dynamics(ld-PaCS-MD)○Hayato Aida (Biology, Life and Earth Sciences, Grad. School of Sci. and Tech., University of Tsukuba),
Yasuteru Shigeta (Center for Computational Sciences, University of Tsukuba), Ryuhei Harada(Center for Computational Sciences, University of Tsukuba)22

P2-14 Comprehensive classification of Protein-peptide interactions in the Protein
Data Bank by using the Gaussian mixture model○Keiichiro Sato (Graduate School of Life Sciences, Ritsumeikan University), Kota Kasahara (Collegeof Life Sciences, Ritsumeikan University), Takuya Takahashi (College of Life Sciences, Ritsumeikan
University)P2-15 Retrieving potential three-dimensional biological shape matches from asmall number of two-dimensional single particle XFEL diffraction patterns○Sandhya P. Tiwari (RIKEN Center for Computational Science), Florence Tama (RIKEN Center for
Computational Science / Institute of Transformative Bio-Molecules, University of Nagoya / Department of
Physics, University of Nagoya), Osamu Miyashita (RIKEN Center for Computational Science)

P2-16 Evaluation of Newly Developed Protein Particle Picking Software for CryoEM○Masataka Ohashi (BioNet Lab. Inc.), Fumio Hosokawa (BioNet Lab. Inc.), Takao Shinkawa(BioNet Lab. Inc.), Kenji Iwasaki (University of Tsukuba)

P2-17 Multi-canonical Molecular Dynamics Study the Fuzzy Complex Formationby the Transcription Factor MED26○Satoshi Goto (Graduate School of Life Sciences, Ritsumeikan University), Kota Kasahara (Collegeof Life Sciences, Ritsumeikan University), Takuya Takahashi (College of Life Sciences, Ritsumeikan
University)P2-18 Dynamics Of Orexin Receptors Using Molecular Dynamics Simulations○Shun Yokoi (Meiji University), Ayori Mitsutake (Meiji University)P2-19 Analysis of Urea-Concentration Dependence for Binding Free Energy ofLysozyme－(GlcNac) 3○Simon Hikiri (Grad. Sch. Eng. Sci., Osaka Univ.), Nobuyuki Matubayasi (Grad. Sch. Eng. Sci.,
Osaka Univ.)

P2-20 Search for Partial Structural Space of Specific Loop Residues by Hydrogen
Bond and Steric Repulsion○Hiroto Murata (Nagoya University), George Chikenji (Nagoya University)P2-21 Evaluation of the protein thermal stability by molecular dynamics
simulation○Kaito Kobayashi (National Institute of Advanced Industrial Science and Technology (AIST)), Tomoshi
Kameda (National Institute of Advanced Industrial Science and Technology (AIST)), Shin Irumagawa

23

(Shinshu University), Ryoichi Arai (Shinshu University), Yutaka Saito (National Institute of Advanced
Industrial Science and Technology (AIST)), Takeshi Miyata (Kagoshima University), Mitsuo Umetsu(Tohoku University)

P2-22 Regulation Mechanism of Agonist/Antagonist Activities of Vitamin D
Receptor Elucidated by Generalized Ensemble Method○Toru Ekimoto (Yokohama City University), Takafumi Kudo (Yokohama City University), Tsutomu
Yamane (Yokohama City University), Mitsunori Ikeguchi (Yokohama City University / RIKEN)

P2-23 PSD-95 multimerization mechanisms studied by coarse-grained molecularsimulations○Yutaka Murata (Grad. Sch. Sci., Kyoto Univ.), Toru Niina (Grad. Sch. Sci., Kyoto Univ.), Shoji
Takada (Grad. Sch. Sci., Kyoto Univ.)

P2-24 Coarse-grained molecular dynamics simulations to dissect effects ofcharge distributions in protein sequences on liquid-liquid phase separation○ Hiroki Terazawa (Graduate School of Life Science, Ritsumeikan University), Kota Kasahara(College of Life Science, Ritsumeikan University), Takuya Takahashi (College of Life Science,
Ritsumeikan University)

P2-25 Evaluating Self-Assembly Propensity of Tetra-Peptide Using MD and
Machine Learning○Yoichi Kurumida (AIRC, AIST), Keisuke Ikeda (Faculty of Pharmaceutical Sciences, University of
Toyama.), Yusuke Nakamichi (ISC, AIST), Kaito Kobayashi (AIRC, AIST), Yutaka Saito (AIRC,
AIST), Tomoshi Kameda (AIRC, AIST)

P2-26 A brand-new particle-picking program: GRIPS○ Junpei Yamaguchi (University of Tsukuba), Masataka Ohashi (BioNet Lab. Inc), Fumio
Hosokawa (BioNet Lab. Inc), Takao Shinkawa (BioNet Lab. Inc), Kenji Iwasaki (University ofTsukuba)

P2-27 Analysis of Reaction Pathway on E1/E2 Transition in SR-Ca2+-ATPase by
Molecular Dynamics Simulations○Chigusa Kobayashi (RIKEN Center for Computational Science), Yasuhiro Matsunaga (Saitama
University), Jaewoon Jung (RIKEN Center for Computational Science / RIKEN Cluster for Pioneering
Research, Theoretical Molecular Science Lab), Yuji Sugita (RIKEN Center for Computational Science /RIKEN Cluster for Pioneering Research, Theoretical Molecular Science Lab / RIKEN Center for Biosystems
Dynamics Research)24

P2-28 Comparison of Molecular Dynamics of Cyclosporin A and Cyclosporin E○ Akari Ito (Yokohama City University), Tsutomu Yamane (Yokohama City University), Toru
Ekimoto (Yokohama City University), Mitsunori Ikeguchi (Yokohama City University / RIKEN)

P2-29 Coarse-grained local potential for disordered proteins optimized via
Bayesian inference○Azuki Mizutani (Dept Biophysics, Div Biology, Grad School Science, Kyoto University), Giovanni
Brandani (Dept Biophysics, Div Biology, Grad School Science, Kyoto University), Shoji Takada (Dept
Biophysics, Div Biology, Grad School Science, Kyoto University)

P2-30 Protein-protein complex dissociation simulated by Parallel Cascade
Selection Molecular Dynamics○Yoshiki Miyazawa (Tokyo Institute of Technology), Duy Phuoc Tran (Tokyo Institute of Technology),
Kazuhiro Takemura (Tokyo Institute of Technology), Akio Kitao (Tokyo Institute of Technology)

P2-31 Local Structural Similarity of Mononucleotide Binding Sites in Different
Levels of SCOPe Classification○Shota Kawakami (Graduate School of Life Sciences, Tohoku University), Hafumi Nishi (Graduate
School of Information Sciences, Tohoku University / Faculty of Core Research, Ochanomizu University),

Kengo Kinoshita (Graduate School of Life Sciences, Tohoku University / Graduate School of Information
Sciences, Tohoku University)P2-32 Computational studies on mechanisms for intramolecular cyclization of Nterminal glutamic acid residues○ Tomoki Nakayoshi (Graduate School of Pharmacy, Meijo University / Institute of Medical,
Pharmaceutical and Health Sciences, Kanazawa University), Koichi Kato (Graduate School of Pharmacy,
Meijo University / Department of Pharmacy, Kinjo Gakuin University), Eiji Kurimoto (Graduate School of
Pharmacy, Meijo University), Akifumi Oda (Graduate School of Pharmacy, Meijo University / Institute ofMedical, Pharmaceutical and Health Sciences, Kanazawa University / Institute for Protein Research, Osaka
University)

P2-33 Comprehensive and Up-to-date Annotation of Nonsynonymous Single
Nucleotide Variants of Human Genome with Protein Structure Information○Matsuyuki Shirota (Tohoku University)

P2-34 Development of Molecular Dynamics Software, GENESIS version 1.4○Yuji Sugita (RIKEN CPR / RIKEN BDR / RIKEN R-CCS), Takaharu Mori (RIKEN CPR), Kiyoshi
Yagi (RIKEN CPR), Hiraku Oshima (RIKEN BDR), Jaewoon Jung (RIKEN R-CCS), Chigusa
Kobayashi (RIKEN R-CCS), Yasuhiro Matsunaga (Saitama University)25

P2-35 Automatic Identification of Subvisible Particles Using Flow Imaging
Microscopy Images○Yuriko Shibano (Department of Biotechnology, Graduate School of Engineering, Osaka University),
Arni Gambe-Gilbuena (Department of Biology, School of Science and Engineering, Ateneo de Manila
University), Elena Krayukhina (Research Department, U-Medico Inc.), Tetsuo Torisu (Department of
Biotechnology, Graduate School of Engineering, Osaka University), Susumu Uchiyama (Department of
Biotechnology, Graduate School of Engineering, Osaka University / Department of Creative Research,
Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences)P2-36 Characterisation of Druggable Cryptic Binding Sites via Atomistic
Molecular Dynamics Simulation○ Shinji Iida (Technology Research Association for Next-Generation Natural Products Chemistry),
Hironori K. Nakamura (Biomodeling Research Co., Ltd.), Tadaaki Mashimo (IMSBIO Co., Ltd.),
Yoshifumi Fukunishi (Molecular Profiling Research Center for Drug Discovery, AIST Tokyo Waterfront)

P2-37 Prediction Of Cancer Associated Hotspot Mutations That Affect GPCR
Oligomerization○Sakie Shimamura (Department of Science and Technology, Tokyo Denki University), Vachiranee
Limviphuvadh (Bioinformatics Institute, Agency for Science, Technology and Research, Singapore),
Yoshihiro Yamanishi (Faculty of Computer Science and Systems Engineering, Department of Bioscienceand Bioinformatics, Kyushu Institute of Technology), Hiroyuki Toh (School of Science and Technology,
Kwansei Gakuin University), Wataru Nemoto (Department of Science and Technology, Tokyo Denki
University)P2-38 Molecular Dynamics Simulation Of The Effect That Ca2+ Has On The Tertiary
Structure Of An RNA Aptamer○Kazuki Kaneta (Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon
University), Seiichiro Ishii (Department of Chemical Biology and Applied Chemistry, College of Engineering,
Nihon University), Hisae Yoshida (Department of Chemical Biology and Applied Chemistry, College ofEngineering, Nihon University / JSPS Research Fellow), Keisuke Masukawa (Department of Chemical
Biology and Applied Chemistry, College of Engineering, Nihon University), Taiichi Sakamoto (Departmentof Life Science, Faculty of Advanced Engineering, Chiba Institute of Technology), Takeshi Ishikawa(Department of Advanced Engineering, Faculty of Engineering, Kagoshima University), Kenji Yamagishi(Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University)

P2-39 The ratio of the mutated proteins among the primary neighboring proteinsof a non-mutated protein follows the Weibull distribution○Eri Hayashi (Tokyo Dennki University), Yuto Kimura (Tokyo Dennki University), Shuichi Hirose(NAGASE & CO., LTD.), Wataru Nemoto (Tokyo Dennki University)26

P2-40 3D-RISM-AI: Prediction of protein-ligand binding free energy based onmachine learning using 3D-RISM○Kazu Ohsaki (Yokohama City University), Ekimoto Toru (Yokohama City University), Yamane
Tsutomu (Yokohama City University), Ikeguchi Mitsunori (Yokohama City University / RIKEN Medical
Science Innovation Hub Program)P2-41 The Relationship between
Local Structures

Designability of Protein and Preference of○Kazuma Toko (Nagoya University), George Chikenji (Nagoya University)P2-42 Comprehensive Analysis of the Hydration of Small Molecule Binding Sites
in Ligand-Free Protein Structures: 3D-RISM Approach○Takashi Yoshidome (Department of Applied Physics, Tohoku University), Mitsunori Ikeguchi(Drug Development Data Intelligence Platform Group, Medical Science Innovation Hub Program, Cluster of
Science, Technology and Innovation Hub, RIKEN / Graduate School of Medical Life Science, Yokohama City
University),

Masateru Ohta (Drug Development Data Intelligence Platform Group, Medical Science

Innovation Hub Program, Cluster of Science, Technology and Innovation Hub, RIKEN)

P2-43 Understanding human genetic variations in protein-protein interactions
through protein and residue sociability○Hafumi Nishi (Graduate School of Information Sciences, Tohoku University / Faculty of Core Research,
Ochanomizu University), Yuki Kagaya (Graduate School of Information Sciences, Tohoku University),
Matsuyuki Shirota (Graduate School of Medicine, Tohoku University), Kengo Kinoshita (Graduate
School of Information Sciences, Tohoku University)

P2-44 Improvement of the prediction of drug sensitivity changes upon mutation
using a molecular dynamics-based alchemical free energy calculation
method○Fumie Ono (Kyoto university), Shuntaro Chiba (RIKEN), Yuta Isaka (Foundation for Biomedical
Shigeyuki Matsumoto (RIKEN), Biao Ma (Foundation for
Biomedical Research and Innovation at Kobe), Ryohei Katayama (Japanese Foundation for Cancer
Research), Mitsugu Araki (Kyoto university), Yasushi Okuno (Kyoto university / RIKEN / Foundation

Research and Innovation at Kobe),

for Biomedical Research and Innovation at Kobe)

P2-45 A Large-scale Structural and Evolutionary Analysis of Protein Loops○Lin Zhang (Graduate School of Information Sciences, Tohoku University), Hafumi Nishi (Graduate
School of Information Sciences, Tohoku University / Faculty of Core Research, Ochanomizu University)27

P2-46 Role of the Cytoplasmic Domains of SLC26A9 in Chloride Ion Transport
Revealed by the Molecular Dynamics Simulations○Satoshi Omori (Graduate School of Information Sciences, Tohoku University), Yuya Hanazono(Institute for Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology),

Hafumi Nishi (Graduate School of Information Sciences, Tohoku University / Faculty of Core Research,
Ochanomizu University), Kengo Kinoshita (Graduate School of Information Sciences, Tohoku University /Tohoku Medical Megabank Organization, Tohoku University / Institute of Development, Aging, and Cancer,
Tohoku University)

P2-47 Prediction of Protein Functional Annotations Using Deep Neural Networkwith Transfer Learning from Pretrained Model with Pfam
○Tsukasa Nakamura (Postdoctoral Fellow, JSPS / Tohoku University), Kengo Kinoshita (Tohoku
University)

P2-48 Database Analysis of Protein-protein Interfaces○Wataru Sagawa (Nagoya University), George Chikenji (Nagoya University)

P2-49 Infinite-swap limit in generalized replica exchange with solute-tempering○Hiroaki Nakayama (Saitama University), Yasuhiro Matsunaga (Saitama University)

P2-50 Bayesian analysis of high-speed atomic force microscopy images towards
integrative modeling of biomolecular dynamics○Tomonori Ogane (Saitama University / JST CREST), Yasuhiro Matsunaga (Saitama University /JST CREST)

P2-51 Fast Calculation of Gaussian Mixture Models by Down Sampled Voxels andAtoms for Fitting Atomic Models on EM Maps
○Takeshi Kawabata (Institute for Protein Research, Osaka University), Haruki Nakamura (Institutefor Protein Research, Osaka University), Genji Kurisu (Institute for Protein Research, Osaka University)28

Protein/Cellular functions, Imaging

P3-01 Multiple binding mechanism: Why can DNA-dameged-binding protein 1bind the substrates with various amino acids?
○Katsumi Omagari (Nagoya City University), Yasuhito Tanaka (Nagoya City University)P3-02 Human Molecular Chaperone Hsp60 apical domain Suppresses
Aggregation and Cytotoxicity of α-synuclein in Eukaryotic cells○Mayuka Adachi (Dep. of Biomed. Sci., Grad Sch. of Med., Tottori University), Hanae Yamamoto(Dep. of Chem. and Biotech., Grad. Sch. of Eng., Tottori University), Kunihiro Hongo (Dep. of Biomed. Sci.,
Grad Sch. of Med., Tottori University / Dep. of Chem. and Biotech., Grad. Sch. of Eng., Tottori University / GSC
Center, Eng., Tottori University), Tomohiro Mizobata (Dep. of Biomed. Sci., Grad Sch. of Med., Tottori
University / Dep. of Chem. and Biotech., Grad. Sch. of Eng., Tottori University / GSC Center, Eng., Tottori
University), Yasushi Kawata (Dep. of Biomed. Sci., Grad Sch. of Med., Tottori University / Dep. of Chem.
and Biotech., Grad. Sch. of Eng., Tottori University / GSC Center, Eng., Tottori University)P3-03 The Analyses of Dynamic Properties of Dnmt1 and Cofactors Based on
All-atom Molecular Dynamics Simulations○ Takunori Yasuda (College of Biological Sciences, University of Tsukuba), Yasuteru Shigeta(Center for Computational Sciences, University of Tsukuba ), Ryuhei Harada (Center for Computational
Sciences, University of Tsukuba)

P3-04 Escherichia coli small heat shock protein IbpA is an aggregation-sensor toself-regulate its own expression at translational levels○ Tsukumi Miwa (School of Life Science and Technology, Tokyo Institute of Technology), Yuhei
Chadani (Cell Biology Center, Tokyo Institute of Technology), Hideki Taguchi (School of Life Scienceand Technology, Tokyo Institute of Technology / Cell Biology Center, Tokyo Institute of Technology)

P3-05 How similar are the LDLR family members? - Examination of pH-dependent
regulation of ligand capture and release through SPR and HS-AFM analyses○Aki Shiozawa (Graduate School of Life Science, Yokohama City University), Noriyuki Kodera (WPI
Nano Life Science Institute, Kanazawa University), Terukazu Nogi (Graduate School of Life Science,
Yokohama City University)P3-06 Observation of Hsp104-driven severing of Huntingtin/PolyQ fiber○Yumi Goto (Department of Biotechnology & Life Science, Tokyo University of Agriculture and Technology),
Kyoka Shibata (Department of Biotechnology & Life Science, Tokyo University of Agriculture andTechnology), Yasuhiro Onoue (Department of Molecular Cell Biology, Institute of Molecular Embryology
and Genetics, Kumamoto University), Teru Ogura (Department of Molecular Cell Biology, Institute of29

Masafumi Yohda (Department of
Biotechnology & Life Science, Tokyo University of Agriculture and Technology), Kyosuke Shinohara
Molecular Embryology and Genetics, Kumamoto University),(Department of Biotechnology & Life Science, Tokyo University of Agriculture and Technology)

P3-07 Development of Acid-tolerant Reversibly Photoswitchable Green
Fluorescent Protein for Super-resolution Imaging in Acidic Environment○Tomoki Matsuda (ISIR, Osaka Univ. / Grad. Sch. Eng., Osaka Univ.), Hajime Shinoda (Grad. Sch.
Eng., Osaka Univ. / CPR, RIKEN), Kai Lu (ISIR, Osaka Univ.), Ryosuke Nakashima (ISIR, Osaka
Univ.), Tetsuichi Wazawa (ISIR, Osaka Univ.), Kosuke Noguchi (Grad. Sch. Eng., Osaka Univ.),
Takeharu Nagai (ISIR, Osaka Univ. / Grad. Sch. Eng., Osaka Univ.)

P3-08 Multistep enzymatic reaction for high-capacity biofuel cells using lacticacid○Ako Kagawa (RIKEN Center for Biosystems Dynamics Research), Takanori Kigawa (RIKEN Center
for Biosystems Dynamics Research), Tsutomu Mikawa (RIKEN Center for Biosystems Dynamics
Research)

P3-09 Functional and Structural Characterization of RTP1S, a Chaperone of
Olfactory Receptors○Ryosuke Inoue (Dept. of Biotechnol., Tokyo Univ of Agri. and Technol.), Yosuke Fukutani (Dept. of
Biotechnol., Tokyo Univ of Agri. and Technol.), Ryohei Tamaki (Dept. of Biotechnol., Tokyo Univ of Agri.
and Technol.), Hiroaki Matsunami (Dept. of Molecular Genetics and Microbiology, Duke Univ. Medical
Center), Masahumi Yohda (Dept. of Biotechnol., Tokyo Univ of Agri. and Technol.)P3-10 The Structural and Functional Diversity of Cholesterol-dependent Cytolysin
Produced from Streptococci○Atsushi Tabata (Department of Bioscience and Bioindustry, Graduate School of Technology, Industrial
and Social Sciences, Tokushima University / Department of Biological Science and Technology, Life System,
Institute of Technology and Science, Tokushima University), Kazuto

Ohkura (Graduate School of
Pharmaceutical Sciences, Suzuka University of Medical Science), Hisashi Ohkuni (Health Science
Research Institute East Japan), Miho Kobayashi (Department of Biological Science and Technology, Life
System, Institute of Technology and Science, Tokushima University), Airi Matsumoto (Department of
Biological Science and Technology, Life System, Institute of Technology and Science, Tokushima University),Toshifumi Tomoyasu (Department of Bioscience and Bioindustry, Graduate School of Technology,
Industrial and Social Sciences, Tokushima University / Department of Biological Science and Technology, Life
System, Institute of Technology and Science, Tokushima University), Hideaki Nagamune (Department of
Bioscience and Bioindustry, Graduate School of Technology, Industrial and Social Sciences, Tokushima
University / Department of Biological Science and Technology, Life System, Institute of Technology and Science,
Tokushima University)30

P3-11 Functional analysis of mammalian odorant receptor produced with thewheat germ cell-free expression system○Masashi Abe (Tokyo University of Agriculture and Technology), Yosuke Fukutani (Tokyo Universityof Agriculture and Technology), Masashi Asakawa (Tokyo University of Agriculture and Technology),
Hiroyuki Takeda (Ehime University), Hiroaki Matsunami (Duke University), Masafumi Yohda(Tokyo University of Agriculture and Technology)

P3-12 Novel Insight into PDI Family-regulated IRE1 Activation/Inactivation○Motonori Matsusaki (Tohoku University), Shingo Kanemura (Kwansei Gakuin University), Kenji
Inaba (Tohoku University), Masaki Okumura (Tohoku University)P3-13 Dynamic features of Canine Distemper Virus H protein revealed by HighSpeed Atomic Force Microscope○Kohei Yumoto (Laboratory of Biomolecular Science, Faculty of Pharmaceutical Science, Hokkaido
University), Hideo Fukuhara (Center for Research and Education on Drug Discovery, Faculty of
Pharmaceutical Science, Hokkaido University), Mihiro Kawamura (Laboratory of Biomolecular Science,
Faculty of Pharmaceutical Science, Hokkaido University), Philippe Plattet (Division of Experimental andClinical Research, Vetsuisse Faculty, University of Bern), Katsumi Maenaka (Laboratory of Biomolecular
Science, Faculty of Pharmaceutical Science, Hokkaido University / Center for Research and Education on Drug
Discovery, Faculty of Pharmaceutical Science, Hokkaido University)P3-14 Isolation and characterization of a linear peptide that binds to human MuSK
extracellular domain○Fumiya Mizutani (Institute for Protein Research, Osaka University), Hayden Peacock (Departmentof Chemistry, School of Science, The University of Tokyo), Kyoko Matoba (Institute for Protein Research,
Osaka University), Osamu Higuchi (Department of Clinical Research, Nagasaki Kawatana Medical Center),
Hiroaki Suga (Department of Chemistry, School of Science, The University of Tokyo), Junichi Takagi(Institute for Protein Research, Osaka University)

P3-15 Msp1 clears mistargeted proteins by facilitating their transfer frommitochondria to the ER○Shunsuke Matsumoto (Kyoto Sangyo University), Kunio Nakatsukasa (Nagoya City University),
Chika Kakuta (Kyoto Sangyo University), Yasushi Tamura (Yamagata University), Masatoshi
Esaki (Kumamoto University), Toshiya Endo (Kyoto Sangyo University)P3-16 Development Of a Single Use In Situ Glutamine Sensor For Bioprocess
Monitoring Using Glutamine Binding Protein○ Shouhei Takamatsu (Tokyo University of Agriculture and Technology), Jinhee Lee (Joint
Department of Biomedical Engineering, The North Carolina University at Chapel Hill and North Carolina State
University), Ryutaro Asano (Tokyo University of Agriculture and Technology), Wakako Tsugawa(Tokyo University of Agriculture and Technology), Koji Sode (Joint Department of Biomedical Engineering,31

The North Carolina University at Chapel Hill and North Carolina State University), Kazunori Ikebukuro(Tokyo University of Agriculture and Technology)

P3-17 Functional analysis of OR7C1 expressed in Colon Cancer-Initiating Cells○Tomoyo Koshizawa (Tokyo University of Agriculture and Technology), Yosuke Fukutani (Tokyo
University of Agriculture and Technology / Duke University Medical Center), Aiko Murai (Sapporo Medical
University), Hiroaki Matsunami (Duke University Medical Center), Yoshihiko Hirohashi (Sapporo
Medical University), Toshihiko Torigoe (Sapporo Medical University), Masafumi Yohda (Tokyo
University of Agriculture and Technology)

P3-18 Cholesterol-dependent cytolysin-like adhesion protein of Mitis group
streptococci○Airi Matsumoto (Department of Biological Science and Technology, Life System, Institute of Technology
and Science, Tokushima University), Atsushi Tabata (Department of Biological Science and Technology,
Life System, Institute of Technology and Science, Tokushima University / Department of Bioscience andBioindustry, Graduate School of Technology, Industrial and Social Sciences, Tokushima University), Ayuko

Takao (Department of Oral Microbiology, School of Dental Medicine, Tsurumi University), Ken Kikuchi(Department of Infectious Diseases, Tokyo Women’s Medical University), Toshifumi Tomoyasu(Department of Biological Science and Technology, Life System, Institute of Technology and Science,
Tokushima University / Department of Bioscience and Bioindustry, Graduate School of Technology, Industrial
and Social Sciences, Tokushima University), Hideaki Nagamune (Department of Biological Science andTechnology, Life System, Institute of Technology and Science, Tokushima University / Department of Bioscienceand Bioindustry, Graduate School of Technology, Industrial and Social Sciences, Tokushima University)P3-19 Visualization of Repeat Associated Non-AUG (RAN) Translation Involved in
Neurodegenerative Diseases at Single mRNA in Living Cells○Hayato Ito (School of Life Science and Technology, Tokyo Institute of Technology), Morio Ueyama(Department of Neurotherapeutics, Osaka University Graduate School of Medicine), Kohji Mori (Departmentof Psychiatry, Osaka University Graduate School of Medicine), Yoshitaka Nagai (Department of
Neurotherapeutics, Osaka University Graduate School of Medicine), Timothy J. Stasevich (Departmentof Biochemistry and Molecular Biology, Institute of Genome Architecture and Function, Colorado State
University / World Research Hub Initiative, Institute of Innovative Research, Tokyo Institute of Technology),

Hideki Taguchi (School of Life Science and Technology, Tokyo Institute of Technology / Cell Biology Center,
Tokyo Institute of Technology)

P3-20 The Molecular Basis of How SNP in LI-cadherin Affected Cancer Metastasis○Anna Yui (Dept. of Bioeng., Sch. of Eng., Univ. of Tokyo), Chika Kikuchi (Dept. of Chem. Biotech.,Sch. of Eng., Univ. of Tokyo), Shuichiro Goda (Grad. Sch. of Eng., Nagasaki Univ.), Shota Kudo (Dept.
of Chem. Biotech., Sch. of Eng., Univ. of Tokyo), Hiroki Akiba (Dept. of Bioeng., Sch. of Eng., Univ. of Tokyo),

Makoto Nakakido (Dept. of Bioeng., Sch. of Eng., Univ. of Tokyo), Daisuke Kuroda (Dept. of Bioeng.,
Sch. of Eng., Univ. of Tokyo / Med. Dev. Dev. Reg. Res. Center, Sch. of Eng., Univ. of Tokyo), Satoru

32

Nagatoishi (Dept. of Bioeng., Sch. of Eng., Univ. of Tokyo / Dept. of Chem. Biotech., Sch. of Eng., Univ. ofTokyo / Inst. of Med. Sci., Univ. of Tokyo), Osamu Arai (RCAST, Univ. of Tokyo), Hiroko Iwanari (RCAST,
Univ. of Tokyo), Takao Hamakubo (RCAST, Univ. of Tokyo), Kouhei Tsumoto (Dept. of Bioeng., Sch.
of Eng., Univ. of Tokyo / Dept. of Chem. Biotech., Sch. of Eng., Univ. of Tokyo / Inst. of Med. Sci., Univ. of Tokyo)P3-21 FAD-binding to apo-form of Synechocystis sp. PCC6803 slr0600 geneproduct in solution○Naho Umeda (Department of Quantum Beam Science, Graduate School of Science and Engineering,
Ibaraki University), Takaaki Suzuki (Department of Quantum Beam Science, Graduate School of Scienceand Engineering, Ibaraki University), Yu Minato (Department of Quantum Beam Science, Graduate Schoolof Science and Engineering, Ibaraki University), Shigenobu Kimura (Department of Quantum Beam
Science, Graduate School of Science and Engineering, Ibaraki University)

P3-22 Bifunctional activity of a fusion enzyme DahX
○Yukiko Takahashi (Graduate School of Life Sciences, Tohoku University), Chihiro Chubachi(Graduate School of Life Sciences, Tohoku University), Shouta Nonoyama (Graduate School of Life
Sciences, Tohoku University), Yukari Sato (Graduate School of Life Sciences, Tohoku University), Yuji
Nagata (Graduate School of Life Sciences, Tohoku University)P3-23 Oligomeric states of MCE transporter components LinM and LinN from
Sphingobium japonicum UT26
○Yukari Sato (Graduate School of Life Sciences, Tohoku University), Takuya Ogata (Graduate Schoolof Life Sciences, Tohoku University), Yuji Nagata (Graduate School of Life Sciences, Tohoku University)

P3-24 PR Poly-dipeptide Blocks the Chaperone Activity of Karyopherinβ2 forFused in Sarcoma○Takeru Uehara (Ritsumeikan University), Ayano Fujiwara (Ritsumeikan University), Takuya
Yoshizawa (Ritsumeikan University), Ryo Uehara (Ritsumeikan University), Hiroyoshi Matsumura(Ritsumeikan University)

P3-25 Identification and characterization of a cuticular protein from the Antarctic
krill Euphausia superba○Tomonobu Seki (Kanagawa University), Miyuki Mekuchi (National Research Institute of Fisheries
Science), Tsuyoshi Matsuda (Port of Nagoya Public Aquarium), Kiyonori Nakajima (Port of Nagoya
Public Aquarium), Nobuo Suzuki (Kanazawa University), Michio Suzuki (The University of Tokyo),
Tsuyoshi Ohira (Kanagawa University)P3-26 Molecular Mechanism of the Recognition of Bacterially
Immunoglobulin by the Immune Regulatory Receptor LILRA2

Cleaved○Atsushi Furukawa (Hokkaido University), Rika Yamazaki (Hokkaido University), Jiaqi Wang

33

(Hokkaido University), Kouyuki Hirayasu (Osaka University / Kanazawa University), Kohei Yumoto(Hokkaido University), Hideo Fukuhara (Hokkaido University), Hisashi Arase (Osaka University),

Katsumi Maenaka (Hokkaido University)

P3-27 Direct observation of Hsp104-driven disassembly of α-synuclein fibril○ Kyoka Shibata (Department of Biotechnology & Life Science, Tokyo University of Agriculture &Technology), Yumi Goto (Department of Biotechnology & Life Science, Tokyo University of Agriculture &Technology), Yasuhiro Onoue (Department of Molecular Cell Biology, Institute of Molecular Embryology
and Genetics, Kumamoto University), Kaori Tsukakoshi (Department of Biotechnology & Life Science,
Tokyo University of Agriculture & Technology), Kazunori Ikebukuro (Department of Biotechnology & Life
Science, Tokyo University of Agriculture & Technology), Masato Hasegawa (Department of Dementia andHigher Brain Function, Tokyo Metropolitan Institute of Medical Science), Teru Ogura (Department of
Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University), Masafumi
Yohda (Department of Biotechnology & Life Science, Tokyo University of Agriculture & Technology),
Kyosuke Shinohara (Department of Biotechnology & Life Science, Tokyo University of Agriculture &Technology)

P3-28 Structural and functional characterization of sHsps from the fission yeast,
Schizosaccharomyces pombe○Rui Nishiima (Tokyo University of Agriculture and Technology), Ken Morishima (Kyoto University),
Rintaro Inoue (Kyoto University), Masaaki Sugiyama (Kyoto University), Masafumi Yohda(Tokyo University of Agriculture and Technology)

P3-29 Immunosuppressive effects of HLA-G2 protein in SLE model mice
○ Hiroshi Watanabe (Hokkaido University), Kimiko Kuroki (Hokkaido University), Chisato
Yamada (Hokkaido University), Yukari Saburi (Hokkaido University), Naoyoshi Maeda (Hokkaido
University), Katsumi Maenaka (Hokkaido University)

P3-30 The Inorganic Phosphate Emerged Upon The Polymerization Of Actin
Filament Affects Its Monomer’s Configuration○Miku Nezasa (Nagaoka University of Technology), Ryusei Ebata (Nagaoka University of Technology),
Takahiro Mitani (Nagaoka University of Technology), Ryota Mashiko (Nagaoka University ofTechnology), Hajime Honda (Nagaoka University of Technology), Kenji Kamimura (National Instituteof Thechnology, Nagaoka College)

P3-31 Analysis of Autophagy-dependent Degradation Mechanism of Cytoplasmic
Chaperonin CCT○Natsuki Ohsako (Tokyo University of Agriculture and Technology), Tomoko Kawamata (Tokyo
Institute of Technology), Yui Jin (Tokyo Institute of Technology), Yoshinori Ohsumi (Tokyo Institute ofTechnology), Masafumi Yohda (Tokyo University of Agriculture and Technology)34

P3-32 Transient Binding And Non-rotational Coupled Motion Of p53 Revealed By
Sub-millisecond Resolved Single-molecule Fluorescence Tracking○Dwiky R. G Subekti (Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku
University / Graduate School of Science, Tohoku University), Agato Murata (Institute of Multidisciplinary
Research for Advanced Materials (IMRAM), Tohoku University / Graduate School of Science, Tohoku University),

Satoshi Takahashi (Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku
University / Graduate School of Science, Tohoku University), Kiyoto Kamagata (Institute ofMultidisciplinary Research for Advanced Materials (IMRAM), Tohoku University / Graduate School of Science,
Tohoku University)

P3-33 Nascent polypeptide-induced ribosome hopping in Escherichia coli
○Aoi Tanabe (School of Life Science and Technology, Tokyo Institute of Technology), Yuhei Chadani(Cell Biology Center, Tokyo Institute of Technology), Hideki Taguchi (School of Life Science and Technology,
Tokyo Institute of Technology / Cell Biology Center, Tokyo Institute of Technology)

P3-34 Requirement of the TOM complex for mitochondrial outer membraneprotein biogenesis in vivo
○Haruka Sakaue (Kyoto Sangyo University), Jiyao Song (University of Freiburg), Toshiya Endo
(Kyoto Sangyo University)

P3-35 Comprehensive analysis of Human Death Domain-fold SuperfamilyInteractome Using Recombinant Protein Array and AlphaScreen○Wei Zhou (Grad Sch Med, Ehime Univ.), Ai Yanase (PROS, Ehime Univ.), Tomoya Nakagita(PROS, Ehime Univ.), Naoe Kaneko (Grad Sch Med, Ehime Univ.), Hiroyuki Takeda (PROS, Ehime
Univ.), Junya Masumoto (Grad Sch Med, Ehime Univ. / PROS, Ehime Univ.)

P3-36 Development of epigenetic enzyme screening using in vitro reconstituted
Drosophila chromatin○Keiichi Okimune (Hokkaido University), Kazuki Matsumoto (Hokkaido University), Nobuaki
Takemori (Ehime University), Szilvia K. Nagy (Semmelweis University), Yaeta Endo (Ehime
University), Taichi Takasuka (Hokkaido University)

P3-37 Expression and functional characterization of reductive dehalogenase from
Dehalococcoides mccartyi CG4○ Reona Higuchi (Tokyo University of Agriculture and Technology), Yoshihiro Tanaka (Tokyo
University of Agriculture and Technology), Jianzhong He (National University of Singapore), Masafumi
Yohda (Tokyo University of Agriculture and Technology)35

P3-38 Functional Analysis Of Membrane Proteins Involved In Iron Absorption
Using Human Intestinal Model Cell System
Michiyo Takahara (Graduate School of Life Science, University of Hyogo), Hitomi Fujishiro (Facultyof Pharmaceutical Sciences, Tokushima Bunri University), Taiho Kambe (Graduate School of Biostudies,
Kyoto University), Hiroshi Sugimoto (Graduate School of Life Science, University of Hyogo / RIKEN
SPring-8 Center), Yoshitsugu Shiro (Graduate School of Life Science, University of Hyogo), ○Hitomi
Sawai (Graduate School of Life Science, University of Hyogo / RIKEN SPring-8 Center)

P3-39 Osmotic Pressure-induced Multilamellar Structure Formation Based On
Protein-Encapsulated Yolk-shell Structure○Tomoki Maruyama (Kyushu University), Yiwei Liu (Kyushu University), Takeshi Mori (Kyushu
University), Yoshiki Katayama (Kyushu University), Akihiro Kishimura (Kyushu University)

P3-40 Dual Function of the Heme Ligation in a Heme-responsive Sensor Protein,
PefR○ Yudai Nishitani (Department of Life Science, University of Hyogo), Megumi Nishinaga(Department of Life Science, University of Hyogo), Seina Nagai (Department of Life Science, University of
Hyogo), Hiroshi Sugimoto (Department of Life Science, University of Hyogo / RIKEN SPring-8 Center),
Takehiko Tosha (Department of Life Science, University of Hyogo / RIKEN SPring-8 Center), Yoshitsugu
Shiro (Department of Life Science, University of Hyogo), Hitomi Sawai (Department of Life Science,
University of Hyogo / RIKEN SPring-8 Center)

P3-41 The landscape of ribosome elongation states revealed by footprint
extension in bacteria○Tomoya Fujita (RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research / Schoolof Life Science and Technology, Tokyo Institute of Technology), Takeshi Yokoyama (Laboratory for Protein
Functional and Structural Biology, RIKEN Center for Biosystems Dynamics Research), Hideki Taguchi(School of Life Science and Technology, Tokyo Institute of Technology / Cell Biology Center, Institute of
Innovative Research, Tokyo Institute of Technology), Takuhiro Ito (Laboratory for Translation Structural
Biology, RIKEN Center for Biosystems Dynamics Research),

Shintaro Iwasaki(RNA Systems

Biochemistry Laboratory, RIKEN Cluster for Pioneering Research / Department of Computational Biology andMedical Sciences, Graduate School of Frontier Sciences, The University of Tokyo)

36

Biophysics, Protein Folding

P4-01 Hydration dynamics of bio-molecules revealed by molecular dynamics
simulations○Takuya T. Takahashi (Dept. Bioinformatics, Ritsumeikan Univ.)

P4-02 Biophysical screening in nanodiscs reveals novel inhibitors of the bacterial
ABC transporter MsbA
○Kaoru Fujimoto (Kyushu University), Satoru Nagatoishi (The University of Tokyo), Kouhei
Tsumoto (The University of Tokyo), Tadashi Ueda (Kyushu University), Jose Caaveiro (Kyushu
University)P4-03 Conformational Dynamics upon Ligand Binding
Acetylcholine Receptor Revealed by FTIR SpectroscopyinMuscarinic○Kota Katayama (Nagoya Institute of Technology / OpotoBioTechnology Reserach Center, Nagoya
Institute of Technology), Kohei Suzuki (Nagoya Institute of Technology), Ryoji Suno (Kansai Medical
University), Hideki Kandori (Nagoya Institute of Technology / OpotoBioTechnology Reserach Center,
Nagoya Institute of Technology)

P4-04 In vivo X-ray single-molecule observation of ice-binding proteins for
Caenorhabditis elegans freezing tolerance○Masahiro Kuramochi (The University of Tokyo / AIST-UTokyo OPERANDO-OIL, AIST), Geikaku
Tou (The University of Tokyo), Motomichi Doi (AIST), Kazuhiro Mio (AIST-UTokyo OPERANDO-OIL,
AIST), Sakae Tsuda (AIST-UTokyo OPERANDO-OIL / AIST), Yuji C. Sasaki (The University of Tokyo /AIST-UTokyo OPERANDO-OIL / JASRI/SPring-8), Hiroshi Sekiguchi (JASRI/SPring-8)

P4-05 Evolution of Protein Solubility in Macromolecular Crowding○ Yasuhiro Isogai (Toyama Prefectural University), Hiroshi Imamura (Ritsumeikan University),
Tomonari Sumi (Okayama University), Ken-ichi Takahashi (Nagahama Institute of Bio-Science andTechnology), Tsuyoshi Shirai (Nagahama Institute of Bio-Science and Technology)

P4-06 Wavelength-dependent reversible interaction dynamics between lightsensor protein PYP and its downstream protein○Suhyang Kim (Kyoto University), Yusuke Nakasone (Kyoto University), Akira Takakado(Gakushuin University), Yoichi Yamazaki (Nara Institute of Science and Technology), Hironari
Kamikubo (Nara Institute of Science and Technology), Masahide Terazima (Kyoto University)37P4-07 Cooperative Regulation Of ClpB Disaggregase By The Middle Domain And
DnaK○Tomonori Murakami (Department of Biology, Graduate School of Natural Science, Konan University),
Takahiro Ishizaki (Department of Biology, Graduate School of Natural Science, Konan University),
Takashi Yamasaki (Institute for Integrative Neurobiology, Konan University), Sayaka Hayashi(Department of Biology, Graduate School of Natural Science, Konan University), Yo-hei Watanabe(Department of Biology, Graduate School of Natural Science, Konan University / Institute for Integrative
Neurobiology, Konan University)

P4-08 Mechanism of Antibody Aggregation by pH-Shift Stress○Hiroshi Imamura (Ritsumeikan University)P4-09 Reduced aggregation and altered N-glycosylation status of recombinant
IgG1 in perfusion mammalian cell culture○Masayoshi Onitsuka (Tokushima University / Manufacturing Technology Association of Biologics(MAB)), Atsuko Shimadu (Manufacturing Technology Association of Biologics (MAB)), Shutaro
Ishikawa (Manufacturing Technology Association of Biologics (MAB) / ABLE-Biott Corporation)

P4-10 Fibrillation of Periplasmic Molecular Chaperone HdeB and its Structural
Characterization○Rina Uehara (Department of Chemistry and Biotechnology, Graduate School of Sustainability Science,
Tottori University), Yui Nakata (Department of Chemistry and Biotechnology, Engineering, Tottori University),

Kunihiro Hongo (Department of Chemistry and Biotechnology, Graduate School of Sustainability Science,
Tottori University / Department of Chemistry and Biotechnology, Engineering, Tottori University), Yasushi
Kawata (Department of Chemistry and Biotechnology, Graduate School of Sustainability Science, Tottori
University / Department of Chemistry and Biotechnology, Engineering, Tottori University), Tomohiro
Mizobata (Department of Chemistry and Biotechnology, Graduate School of Sustainability Science, Tottori
University / Department of Chemistry and Biotechnology, Engineering, Tottori University)

P4-11 Relation of Colloidal and Conformational Stabilities to Aggregate Formation
in a Monoclonal Antibody○ Hiroaki Oyama (Osaka University), Hiroki Koga (Osaka University), Takashi Tadokoro(Hokkaido University), Katsumi Maenaka (Hokkaido University), Akira Shiota (Institute of Immunology
Co., Ltd.), Masami Yokoyama (U-Medico Inc.), Masanori Noda (U-Medico Inc.), Tetsuo Torisu(Osaka University), Susumu Uchiyama (Osaka University / ExCELLS)

38

P4-12 Understanding the mechanism governing protein folding assisted by interor intra- molecular chaperon○Koki Masuda (Sch. of Sci. and Tech., Kwansei Gakuin Univ.), Shingo Kanemura (Sch. of Sci. andTech., Kwansei Gakuin Univ.), Hiroshi Yamaguchi (Sch. of Sci. and Tech., Kwansei Gakuin Univ.), Yuji
Hidaka (Fac. of Sci. and Eng., Kindai Univ.), Kenji Inaba (IMRAM, Tohoku Univ.), Masaki Okumura(FRIS, Tohoku Univ.)

P4-13 Structural difference and its seed-dependent propagation manner ofhuman/bovine insulin amyloid fibrils as detected by iodine staining○Keisuke Yuzu (Graduate School of Science, Kobe University), Naoki Yamamoto (Faculty of
Medicine, Jichi Medical University), Masahiro Noji (Institute for Protein Research, Osaka University),
Masatomo So (Institute for Protein Research, Osaka University), Yuji Goto (Institute for Protein
Research, Osaka University), Tetsushi Iwasaki (Biosignal Research Center, Kobe University), Motonari
Tsubaki (Graduate School of Science, Kobe University), Eri Chatani (Graduate School of Science, Kobe
University)P4-14 Construction Of Synthetic Nanopores With De Novo Design Of α-helical
Peptide○Masataka Usami (Tokyo University of Agriculture and Technology), Keisuke Shimizu (Tokyo
University of Agriculture and Technology), Yusuke Sekiya (Tokyo University of Agriculture and Technology),
Ryuji Kawano (Tokyo University of Agriculture and Technology)

P4-15 Identification of Cyclin-dependent kinase-like 5 substrate protein using
docking simulation technique○Aya Takahara (Ritsumeikan University College of Life Sciences), Shoichi Katayama (Ritsumeikan
University College of Pharmaceutical Sciences), Takako Kawano (Ritsumeikan University College of
Pharmaceutical Sciences), Tetsuya Inazu (Ritsumeikan University College of Pharmaceutical Sciences),

Takeshi Kikuchi (Ritsumeikan University College of Life Sciences)P4-16 Mechanism of polyphosphate-induced amyloid fibrillization of α -synuclein○Keiichi Yamaguchi (Institute for Protein Research, Osaka University), Masatomo So (Institute forProtein Research, Osaka University), Kenji Sasahara (Institute for Protein Research, Osaka University),
Yasushi Kawata (Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori
University), Yuji Goto (Institute for Protein Research, Osaka University)P4-17 Inhibition of P-cadherin-mediated Cell Contact Formation Through The
Kinetic Effect Of Chemical Fragment○Akinobu Senoo (Dept. of Chem. Biotech., Grad. Sch. of Eng., Univ. of Tokyo), Yutaro Saito (Dept.
of Chem. Biotech., Grad. Sch. of Eng., Univ. of Tokyo), Sho Itoh (RIKEN, SPring-8), Satoru Nagatoishi(Inst. of Med. Sci., Univ. of Tokyo), Go Ueno (RIKEN, SPring-8), Kouhei Yoshida (Dept. of Bioeng., Grad.
Sch. of Eng., Univ. of Tokyo), Takumi Tashima (Dept. of Chem. Biotech., Grad. Sch. of Eng., Univ. of Tokyo),39

Shota Kudo (Dept. of Chem. Biotech., Grad. Sch. of Eng., Univ. of Tokyo), Shisuke Sando (Dept. of
Chem. Biotech., Grad. Sch. of Eng., Univ. of Tokyo), Kouhei Tsumoto (Dept. of Chem. Biotech., Grad. Sch.
of Eng., Univ. of Tokyo / Inst. of Med. Sci., Univ. of Tokyo / Dept. of Bioeng., Grad. Sch. of Eng., Univ. of Tokyo)P4-18 The Effect of Post-Translational Modifications of Histone H3 Tail on LiquidLiquid Phase Separation of DNA○Masahiro Mimura (Pure and Appl. Sci., Univ. Tsukuba / Biomed. Res. Inst., AIST), Shunsuke
Tomita (Biomed. Res. Inst., AIST), Hiroka Sugai (Biomed. Res. Inst., AIST), Yoichi Shinkai (Biomed.
Res. Inst., AIST), Ryoji Kurita (Pure and Appl. Sci., Univ. Tsukuba / Biomed. Res. Inst., AIST)

P4-19 Assembly Mechanism of Haemophilus Influenzae Adhesin Transmembrane
Domain into BamA-embedded Nanodiscs○ Eriko Aoki (Department of Bioinformatics, Soka University), Kazuo Fujiwara (Department of
Bioinformatics, Soka University), Masamichi Ikeguchi (Department of Bioinformatics, Soka University)

P4-20 Structural analysis of a therapeutic protein at air-liquid interface○Kanta Enomoto (Department of Biotechnology, Graduate School of Engineering, Osaka University),
Tetsuo Torisu (Department of Biotechnology, Graduate School of Engineering, Osaka University),
Susumu Uchiyama (Department of Biotechnology, Graduate School of Engineering, Osaka University /Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences)P4-21 Conformational analysis for ubiquitin polymers upon phosphorylation○Yuka Yamashita (Dept. of Biomed. Eng., Shinshu Univ.), Reina Takahashi (Dept. of Biomed. Eng.,
Shinshu Univ.), Koji Umezawa (Dept. of Biomed. Eng., Shinshu Univ. / IBS, Shinshu Univ.)

P4-22 The aggregation pathways and mechanisms of serpin polymers○Mone Yonekura (Osaka Prefecture University), Yuki Nishikawa (Osaka Prefecture University),
Saki Hirohata (Osaka Prefecture University), Maki Onda (Osaka Prefecture University)P4-23 Water-amide proton exchange coupled with protein conformationaltransition studied by high-pressure NMR○Soichiro Kitazawa (College of Pharmaceutical Sciences, Ritsumeikan University), Ryo Kitahara(College of Pharmaceutical Sciences, Ritsumeikan University)

P4-24 Unfolding Mechanism of a Lytic Polysaccharide Monooxygenase from
Serratia marcescence, CBP21
Yuichi Nakajima (Niigata University), Ayaka Motoyama (Niigata University), Takeshi Watanabe(Niigata University), Kazushi Suzuki (Niigata University), ○Hayuki Sugimoto (Niigata University)40

P4-25 Construction And Functional Characterization Of Heterodimeric KaiA, An
Activator Of Circadian Clock Protein KaiC
○Yasuhiro Onoue (College of Life Sciences, Ritsumeikan University), Kotona Fujie (College of Life
Sciences, Ritsumeikan University), Kazuki Terauchi (College of Life Sciences, Ritsumeikan University /Graduate School of Life Sciences, Ritsumeikan University)P4-26 Biochemical characterizations of ER-resident peroxidases, GPx7 and GPx8,
reveal their distinct oxidative activities○Shingo Kanemura (Sch. of Sci. and Tech., Kwansei Gakuin Univ. / IMRAM, Tohoku Univ. / FRIS, Tohoku
Univ.), Elaza Firdiani Sofia (IMRAM, Tohoku Univ.), Naoya Hirai (IMRAM, Tohoku Univ.), Masaki

Okumura (IMRAM, Tohoku Univ. / FRIS, Tohoku Univ.), Hiroshi Kadokura (IMRAM, Tohoku Univ.),
Kenji Inaba (IMRAM, Tohoku Univ.)

P4-27 Single Molecule Observation of Amyloid β Peptide on Lipid Membrane
under Non-equilibrium Fluidic Condition○Akane Iida (Graduate school of Science and Engineering, Yamagata University), Kei Unoura (Facultyof Science, Yamagata University), Hideki Nabika (Faculty of Science, Yamagata University)

P4-28 The Effect of Antibiotic Minocycline on Human Insulin Amyloid○Wakako Mori (Ehime University), Keisuke Yuzu (Ehime University), Keiichi Iwaya (SASAKI
Institute, Kyoundo Hospital), Terumasa Nagase (Tokyo Medical University Ibaraki Medical Center),Nadine Lobsiger (Ehime University), Michael Lindgren (Norwegian University of Science andTechnology), Sofie Nyström (Linköping University), Peter Nilsson (Linköping University), Per
Hammarström (Linköping University), Tamotsu Zako (Ehime University)

P4-29 Interaction between Mint3 and FIH-1 analyzed by high-resolution NMR○Ryo Maeda (Kyoto University), Satoru Nagatoishi (The University of Tokyo), Kouhei Tsumoto(The University of Tokyo), Katsumi Matsuzaki (Kyoto University), Masaru Hoshino (Kyoto University)

P4-30 Amyloid Formation And Breakdown Of Supersaturation○Masahiro Noji (Kyoto University), Keiichi Yamaguchi (Osaka University), Eri Chatani (Kobe
University), Yuji Goto (Osaka University)

P4-31 Engineering of TOP7 aiming for improving diffraction-quality crystal
formation○Yuki Ito (Yamagata University), Shota Shiga (Yamagata University), Koki Makabe (Yamagata
University)41

P4-32 Unfolding-dependent Disulfide-linked Dimer Formation Leads To The
Transthyretin Amyloid Assembly Pathway Toward The Production Of
Cytotoxic Aggregate○Yuki Inada (Department of Analytical and Biophysical Chemistry, Graduate School of Pharmaceutical
Sciences, Kumamoto University), Takashi Sato (Department of Analytical and Biophysical Chemistry,
Graduate School of Pharmaceutical Sciences, Kumamoto University), Kentaro Noi (Institute for
NanoScience Design, Osaka University), Ryoko Sasaki (Department of Molecular Medicine, Graduate
School of Pharmaceutical Sciences, Kumamoto University), Yuichiro Ono (Department of Analytical andBiophysical Chemistry, School of Pharmacy, Kumamoto University), Yoshihiro Kobashigawa(Department of Analytical and Biophysical Chemistry, Graduate School of Pharmaceutical Sciences, Kumamoto
University), Tsuyoshi Shuto (Department of Molecular Medicine, Graduate School of Pharmaceutical
Sciences, Kumamoto University), Hirofumi Kai (Department of Molecular Medicine, Graduate School of
Pharmaceutical Sciences, Kumamoto University),

Hiroshi Morioka (Department of Analytical andBiophysical Chemistry, Graduate School of Pharmaceutical Sciences, Kumamoto University)

P4-33 Simplified method for production of cyclic single-chain Fv antibodies byusing a sophisticated Escherichia coli expression system○ Chenjiang Liu (Graduate School of Pharmaceutical Sciences, Kumamoto University), Soichiro
Yamauchi (Graduate School of Pharmaceutical Sciences, Kumamoto University), Natsuki Fukuda(Graduate School of Pharmaceutical Sciences, Kumamoto University), Takashi Sato (Graduate School of
Pharmaceutical Sciences, Kumamoto University), Takeshi Masuda (Graduate School of Pharmaceutical
Sciences, Kumamoto University), Yoshihiro Kobashigawa (Graduate School of Pharmaceutical
Sciences, Kumamoto University), Sumio Ohtsuki (Graduate School of Pharmaceutical Sciences,
Kumamoto University), Hiroshi Morioka (Graduate School of Pharmaceutical Sciences, Kumamoto
University)

P4-34 Structures and dynamics of bacterial actin homolog, MreB proteins
involved in swimming motility of Spiroplasma eriocheiris○Daichi Takahashi (Graduate School of Science, Osaka City University), Ikuko Fujiwara (Graduate
School of Science, Osaka City University), Katsumi Imada (Graduate School of Science, Osaka University),
Makoto Miyata (Graduate School of Science, Osaka City University / OCARINA, Osaka City University)

P4-35 Conformational Characteristic Analysis Of β2-microglobulin Amyloid Fibril
Precursor Using Pressure NMR○Ryousuke Tomiyama (Kindai University), Kazumasa Sakurai (Kindai University)

P4-36 Analyses of pH-dependent residual structure and aggregate’s morphologyof α-synuclein○ Hiroki Ito (Kindai University), Tomotaka Sasaki (Kindai University), Satoshi Abe (Kindai
University), Young-Ho Lee (Korea Basic Sci. Inst.), Kazumasa Sakurai (Int. Adv. Tech, Kindai
University)42P4-37 Mechanisms of Cryoprotection and Amyloid Formation Inhibition of
Human-Derived Intrinsically Disordered Proteins: A Challenge for
Molecular Shield Mechanism
Koki Ikeda (Nagoya University), Shota Suzuki (Nagoya University), Naoki Matsuo (Nagoya
University), Yoshiki Shigemitsu (Tokyo Institute of Technology), Natsuko Goda (Nagoya University),
Takeshi Tenno (Nagoya University / BeCellBar, LLC.), Atsunori Oshima (Nagoya University), ○Hidekazu Hiroaki (Nagoya University / BeCellBar, LLC.)

P4-38 Utilization of Multi-domain Enzimatic Scaffold of Cytochrome P450BM3 for
Molecular Oxygen Activation with Non-biological Porphyrin Complex○Keita Omura (Nagoya University), Yuichiro Aiba (Nagoya University), Joshua Kyle Stanfield(Nagoya University), Shinya Ariyasu (Nagoya University), Hiroshi Sugimoto (JST-CREST / RIKEN
SPring-8), Reza Ghiladi (North Carolina State University), Yoshihito Watanabe (Nagoya University),
Osami Shoji (Nagoya University / JST-CREST)

P4-39 Prediction of Folding Pathways of Multi-domain Proteins by an Extended
Statistical Mechanical Model○Koji Ooka (Department of Physics, The University of Tokyo), Munehito Arai (Department of Physics,
The University of Tokyo / Department of Life Sciences, The University of Tokyo)P4-40 Effect Of Peroxynitric Acid On Aggregation Of Amyloid β Protein○Kawabe Haruka (Ehime University), Ikawa Satoshi (Osaka Research Institute of Industrial Scienceand Technology), Kitano Katsuhisa (Osaka University), Zako Tamotsu (Ehime University)

P4-41 Biosensors for morphinan alkaloids based on their biosynthetic enzymes○ Mei Nonoshita (Chiba University), Yuki Kimura (Chiba University), Yusuke Otani (Chiba
University), Ikki Kobayashi (Chiba University), Akira Nakagawa (Ishikawa Prefectural University),
Hiromichi Minai (Ishikawa Prefectural University), Shigeko Kawai(Noma) (Chiba University),
Daisuke Umeno (Chiba University)

P4-42 Identification of GroE function at low temperature○Yusuke Ichinokawa (Tokyo Institute of Technology), Tatsuya Niwa (Tokyo Institute of Technology),
Kurumi Watanabe (Tokyo Institute of Technology), Naomi Ledey (Tokyo Institute of Technology),
Hedeki Taguchi (Tokyo Institute of Technology)

P4-43 Characterization of the Complex of Aggregated Antibody and Silicone Oil
Droplets; From Perspectives of Morphology, 3-D View, and FγRs activation○Masato Kiyoshi (National Institute of Health Sciences), Minoru Tada (National Institute of Health
Sciences), Hiroko Shibata (National Institute of Health Sciences), Akiko Ishii-Watabe (National
Institute of Health Sciences), Michihiko Aoyama (National Institute of Health Sciences)43

P4-44 N-terminal region of Drosophila Argonaute2 can form amyloid fibrils○ Haruka Narita (The University of Tokyo), Makoto F. Kuwabara (The University of Tokyo),
Tomotaka Komori (The University of Tokyo), Ryo Murakami (The University of Tokyo), Tomohiro
Shima (The University of Tokyo), Mikiko C. Siomi (The University of Tokyo), Sotaro Uemura (The
University of Tokyo)P4-45 Residue-specific NMR analysis of a polypeptide chain revealed a new-typelinear free energy relationship○ Seiichiro Hayashi (Kyushu University), Daisuke Fujinami (Kyushu University), Daisuke
Kohda (Kyushu University)P4-46 The H/D-Exchange Kinetics of Unfolded Ubiquitin in 6 M Guanidinium
Chloride Studied by the DMSO-Quenched 2D NMR Techniques○Kunihiro Kuwajima (The University of Tokyo / Korea Institute for Advanced Study), Maho YagiUtsumi (Institute for Molecular Science), Saeko Yanaka (Institute for Molecular Science), Koichi Kato(Institute for Molecular Science)

P4-47 Study on Membrane-Bound Conformation of α-Synuclein using VacuumUltraviolet Circular-Dichroism Spectroscopy and Molecular Dynamics
Simulation○Ryota Imaura (Department of Physical Science, Graduate School of Science, Hiroshima University),
Munehiro Kumashiro (Department of Physical Science, Graduate School of Science, Hiroshima
University), Yasushi Kawata (Department of Chemistry and Biotechnology, Graduate School ofEngineering, Tottori University), Koichi Matsuo (Hiroshima Synchrotron Radiation Center, Hiroshima
University)

P4-48 Effect of Membrane Thickness on Magainin 2-Induced Pore Formation
Characterized by Vacuum-Ultraviolet Circular-Dichroism and LinearDichroism Spectroscopy
Shoma Suenaga (Hiroshima University), ○Munehiro Kumashiro (Hiroshima University), Koichi
Matsuo (Hiroshima University)

P4-49 Misfolded Wild-type SOD1 in Cerebrospinal Fluid is a Common PathologicalSpecies in Amyotrophic Lateral Sclerosis
Eiichi Tokuda (Department of Chemistry, Keio University), Shinji Ohara (Department of Neurology, Iida
Hospital), Noriko Fujiwara (Hyogo College of Medicine), Isao Hozumi (Gifu Pharmaceutical University),○Yoshiaki Furukawa (Department of Chemistry, Keio University)44

P4-50 CoDN3, an Effector of Colletotrichum Orbiculare, Is an IDP (Intrinsically
Disordered Protein) That Binds to Calmodulin in a Ca2+ Dependent Manner○Shinya Ohki (Japan Advanced Institute of Science and Technology), Noriyoshi Isozumi (Japan
Advanced Institute of Science and Technology), Yoshihiro Inoue (Kyoto University), Tomohiro
Imamura (Ishikawa Prefectural University), Masahi Mori (Ishikawa Prefectural University), Yoshitaka
Takano (Kyoto University)

P4-51 Relationship between helix stability and closed loop length○Yuuki Yanagida (Soka University), Kiyomi Yoshida (Soka University), Kazuo Fujiwara (Soka
University), Masamichi Ikeguchi (Soka University)45

Proteomics / Protein engineering

P5-01 Generation of Highly Functionalized Antibodies by Site-specific Chemical
Conjugation Using an Affinity Peptide Called CCAP
○Yuji Ito (Kagoshima University)P5-02 Expanding the Substrate Specificity of Cytochrome P450 Peroxygenases
by Substrate Misrecognition○Hiroki Onoda (Graduate School of Medical LifeScience, Yokohama City University), Osami Shoji(Graduate School of Science, Nagoya University / CREST, Japan Science and Technology Agency), Hiroshi
Sugimoto (CREST, Japan Science and Technology Agency / RIKEN SPring-8 Center), Yoshitsugu Shiro(Graduate School of Life Science, University of Hyogo), Yoshihito Watanabe (Graduate School of Science,
Nagoya University)P5-03 Construction of Structure-Switchable Protein Supramolecules using
Fusion Proteins Designed Based on 3D Domain Swapping○Masaru Yamanaka (Nara Institute Science and Technology), Takayuki Uchihashi (Nagoya
University), Shun Hirota (Nara Institute Science and Technology)

P5-04 The design of artificial domain-swapped dimers by polyproline rod
insertion○ Shota Shiga (Graduate School of Science and Engineering, Yamagata University), Masaru
Yamanaka (Division of Materials Science, Nara Institute of Science and Technology), Wataru Fujiwara(Graduate School of Science and Engineering, Yamagata University), Shun Hirota (Division of Materials
Science, Nara Institute of Science and Technology), Shuichiro Goda (Graduate School of Engineering,Nagasaki University), Koki Makabe (Graduate School of Science and Engineering, Yamagata University)

P5-05 Dual-Surface Functionalization of an Artificial Protein Nanocapsule Basedon Molecular Sieving Effect of its Porous Surface○Erika Nasu (Keio University), Norifumi Kawakami (Keio University), Kenji Miyamoto (Keio
University)P5-06 Improvements in sensitivity of immunoassay for Legionella through anantibody engineering of VHH antibodies○Norihiko Kiyose (Kagoshima University), Yuji Ito (Kagoshima University)46

P5-07 New Strategy For Designing Direct Electron Transfer Type Enzyme by
Structure-based Protein Engineering○Kohei Ito (Tokyo University of Agriculture and Technology), Junko Okuda-Shimazaki (Universityof North Carolina at Chapel Hill, USA), Wakako Tsugawa (Tokyo University of Agriculture and Technology),
Ryutaro Asano (Tokyo University of Agriculture and Technology), Tsubasa Hashimoto (Tohoku
University), Takeshi Yokoyama (Tohoku University), Yoshikazu Tanaka (Tohoku University), Bruno
Humbel (Okinawa Institute of Science and Technology Graduate University), Ryo Kanno (Okinawa
Institute of Science and Technology Graduate University), Kazunori Ikebukuro (Tokyo University ofAgriculture and Technology), Koji Sode (University of North Carolina at Chapel Hill, USA)P5-08 Incorporation of Hydrophobic Small Molecules in the Artificial Protein
Nanoparticle TIP60○Norifumi Kawakami (Keio University), Maika Yamashita (Keio University), Kenji Miyamoto(Keio University)

P5-09 Relationship between protein adsorption and protein aggregation inbiopharmaceuticals○Saki Yoneda (Osaka University), Ayana Hioki (Osaka University), Takahiro Maruno (Osaka
University), Haruka Nishiumi (Osaka University), Asuka Mori (Osaka University), Taichi
Sawaguchi (ZEON Corporation), Yosuke Harauchi (ZEON Corporation), Susumu Uchiyama(Osaka University)

P5-10 Double-CDR Grafting for the Development of Small HER2-Targeting
Proteins○Kyra T. See (Tokyo Institute of Technology), Tetsuya Kadonosono (Tokyo Institute of Technology),
Wanaporn Yimchuen (Tokyo Institute of Technology), Shinae Kizaka-Kondoh (Tokyo Institute ofTechnology)

P5-11 Development of a Bioluminescent Imaging Probe for Detecting KISS1RExpressing Cancers In Vivo
○Koya Honma (Tokyo Institute of Technology), Tetsuya Kadonosono (Tokyo Institute of Technology),
Wanaporn Yimchuen (Tokyo Institute of Technology), Shinae Kizaka-Kondoh (Tokyo Institute ofTechnology)

P5-12 Effect Of 5' UTR Of The Template DNA On Protein Synthesis With The
Reconstituted Cell-free Protein Synthesis System (PUREfrex)○ Takashi Kanamori (GeneFrontier Corporation), Tomoe Fuse-Murakami
Corporation), Rena Matsumoto (GeneFrontier Corporation)(GeneFrontier47

P5-13 Modification Of The AAV2 Capsid With PA-Tag Insertion○Sarina Ando (Osaka University), Junichi Takagi (Osaka University), Emiko Mihara (Osaka
University), Satoshi Watanabe (Osaka University)

P5-14 Improved conformational stability and resistance against aggregation of
backbone-circularized protein by gains in enthalpy of folded structure andcompactness of unfolded structure○Risa Shibuya (BMRI, AIST), Takamitsu Miyafusa (BMRI, AIST), Shinya Honda (BMRI, AIST
/ Grad. Sch. of Fro., Univ. of Tokyo)

P5-15 Optimization Of The Expression Construct For Viral CD47 Homologues○Yurie Noguchi (Osaka University), Junichi Takagi (Osaka University)P5-16 Function of the segments in coiled-coils of yeast cargo receptors
Emp46p/47p in their assembly and possibility of application to pHdependent biosensor○Koichi Kato (Facul. of Pharm., Meijo Univ. / Col. of Pharm., Kinjo Gakuin Univ.), Tomoki Nakayoshi(Facul. of Pharm., Meijo Univ.), Eiji Kurimoto (Facul. of Pharm., Meijo Univ.), Akifumi Oda (Facul. of
Pharm., Meijo Univ. / Inst. for Prot. Res., Osaka Univ. )P5-17 Affinity maturation of a cleft-binding VHH by a computer-guided library○Hiroki Akiba (National Institutes of Biomedical Innovation, Health and Nutrition / Kyoto University),
Hiroko Tamura (The University of Tokyo), Jose M.M. Caaveiro (Kyushu University), Kouhei
Tsumoto (National Institutes of Biomedical Innovation, Health and Nutrition / The University of Tokyo)

P5-18 Development of tumor specific drug delivery system by multimerization of
lipocalin-type prostaglandin D synthase○Haruna Yoshida (Grad. Sch. of Life & Envi. Sci., Osaka Pref. Univ.), Naohiro Shimoji (Grad. Sch.
of Life & Envi. Sci., Osaka Pref. Univ.), Kosuke Furuta (Grad. Sch. of Life & Envi. Sci., Osaka Pref. Univ.),
Rina Okubo (Grad. Sch. of Life & Envi. Sci., Osaka Pref. Univ.), Sayumi Tashiro (Grad. Sch. of Life &Envi. Sci., Osaka Pref. Univ.), Masatoshi Nakatsuji (Grad. Sch. of Life & Envi. Sci., Osaka Pref. Univ.),
Shigenori Nishimura (Grad. Sch. of Life & Envi. Sci., Osaka Pref. Univ.), Takashi Inui (Grad. Sch. ofLife & Envi. Sci., Osaka Pref. Univ.)

P5-19 Drug Delivery System for Anti-Cancer Drug Paclitaxel Using Human
Lipocalin-type Prostaglandin D Synthase○Kosuke Furuta (Grad. Sch. of Life & Envi. Sci., Osaka Pref. Univ.), Masatoshi Nakatsuji (Grad.
Sch. of Life & Envi. Sci., Osaka Pref. Univ.), Haruna Yoshida (Grad. Sch. of Life & Envi. Sci., Osaka Pref.
Univ.), Rina Okubo (Grad. Sch. of Life & Envi. Sci., Osaka Pref. Univ.), Sayumi Tashiro (Grad. Sch. ofLife & Envi. Sci., Osaka Pref. Univ.), Takashi Inui (Grad. Sch. of Life & Envi. Sci., Osaka Pref. Univ.)48

P5-20 Analysis of immune response induced by protein aggregates derived fromthree types of syringes○Haruka Nishiumi (Osaka University), Saki Yoneda (Osaka University), Takahiro Maruno(Osaka University), Masami Yokoyama (U-Medico Inc), Taichi Sawaguchi (Zeon Corporation),
Yosuke Harauchi (Zeon Corporation), Susumu Uchiyama (Osaka University)P5-21 Structural basis for antigen recognition by methylated lysine specificantibodies
Misaki Ishii (University of Tokyo), ○ Makoto Nakakido (University of Tokyo), Jose M.M.
Caaveiro (Kyushu University), Daisuke Kuroda (University of Tokyo), CJ Okumura (Abwizbio Inc.),
Toshiaki Maruyama (Abwizbio Inc.), Kevin Entzminger (Abwizbio Inc.), Kouhei Tsumoto(University of Tokyo)

P5-22 Proteome Analysis to Clarify the Function of the Translation Initiation
Factors in Escherichia coli
○Masamu Wakabayashi (School of Life Science and Technology, Tokyo Institute of Technology),
Yuhei Chadani (Cell Biology Center, Tokyo Institute of Technology), Tatsuya Niwa (Cell Biology Center,
Tokyo Institute of Technology / School of Life Science and Technology, Tokyo Institute of Technology), Hideki
Taguchi (Cell Biology Center, Tokyo Institute of Technology / School of Life Science and Technology, Tokyo
Institute of Technology)

P5-23 Stabilization of engineered lactate oxidase by introducing inter-subunit
disulfide bond
○ Kentaro Hiraka (Tokyo University of Agriculture and Technology), Wakako Tsugawa (Tokyo
University of Agriculture and Technology), Ryutaro Asano (Tokyo University of Agriculture and Technology),
Kazunori Ikebukuro (Tokyo University of Agriculture and Technology), Koji Sode (Joint Department of
Biomedical Engineering, The University of North Carolina at Chapel Hill, North Carolina State University)

P5-24 Enzymatic crosslinking of small antibody and cationic peptide for siRNAdelivery○Hikaru Nakazawa (Tohoku University), Yu Ando (Tohoku University), Disuke Miura (Tohoku
University), Mitsuo Umetsu (Tohoku University)

P5-25 Development Of Novel Preparation Method For Bispecific Antibody Using
Dimerizing Motif And Refolding Procedure○ Linko Hirono (Tokyo University of Agriculture and Technology), Kazunori Ikebukuro (Tokyo
University of Agriculture and Technology), Izumi Kumagai (Tokyo University of Agriculture and Technology),
Ryutaro Asano (Tokyo University of Agriculture and Technology)49

P5-26 Structural analysis of a peptide fragment grafted onto the Adhiron scaffold○Taichi Sumikawa (Dept. of Bioeng., Sch. of Eng,. Univ. of Tokyo), Makoto Nakakido (Dept. of
Bioeng., Sch. of Eng,. Univ. of Tokyo / Dept. of Chem. Biotech., Sch. of Eng., Univ. of Tokyo), Daisuke
Kuroda (Dept. of Bioeng., Sch. of Eng,. Univ. of Tokyo / Dept. of Chem. Biotech., Sch. of Eng., Univ. of Tokyo/ Med. Dev. Dev. Reg. Res. Center, Sch. of Eng., Univ. of Tokyo), Kouhei Tsumoto (Dept. of Bioeng., Sch.
of Eng,. Univ. of Tokyo / Dept. of Chem. Biotech., Sch. of Eng., Univ. of Tokyo / Med. Dev. Dev. Reg. Res. Center,
Sch. of Eng., Univ. of Tokyo / Inst. of Med. Sci., Univ. of Tokyo)

P5-27 Engineering Of Genome Editing Protein Cas9 That Slides Along DNA Faster
And Might Enable Efficient Target Search○Trishit Banerjee (Institute for Multidisciplinary Research for Advanced Materials, Tohoku University /Department of Chemistry, Graduate School of Science, Tohoku University), Dwiky Rendra Graha
Subekti (Institute for Multidisciplinary Research for Advanced Materials, Tohoku University / Department ofChemistry, Graduate School of Science, Tohoku University), Hiroto Takahashi (Institute forMultidisciplinary Research for Advanced Materials, Tohoku University), Satoshi Takahashi (Institute forMultidisciplinary Research for Advanced Materials, Tohoku University), Kiyoto Kamagata (Institute forMultidisciplinary Research for Advanced Materials, Tohoku University)

P5-28 Proteomic Identification And Validation Of New Interactions Of Novel Tumor
Suppressor PRELP With Membrane Proteins○Hirofumi Kosuge (Dept. of Chem. Biotech., Sch. of Eng., Univ. of Tokyo), Makoto Nakakido (Dept.
of Chem. Biotech., Sch. of Eng., Univ. of Tokyo / Dept. of Bioeng., Sch. of Eng,. Univ. of Tokyo), Satoru
Nagatoishi (Dept. of Chem. Biotech., Sch. of Eng., Univ. of Tokyo / Dept. of Bioeng., Sch. of Eng,. Univ. ofTokyo / Inst. of Med. Sci., Univ. of Tokyo), Tetsuya Fukuda (Biosys Technologies), Yasuhiko Bando(Biosys Technologies), Shin-ichi Ohnuma (Inst. of Ophthalmology, Univ. College London), Kouhei
Tsumoto (Dept. of Chem. Biotech., Sch. of Eng., Univ. of Tokyo, Dept. of Bioeng., Sch. of Eng,. Univ. of Tokyo,
Inst. of Med. Sci., Univ. of Tokyo)P5-29 Metal Inducive Assembly of a Newly Designed Protein Cage TIP60○Naoya Ohara (Keio University), Norifumi Kawakami (Keio University), Kenji Miyamoto (Keio
University)

P5-30 Construction and Characterization of the Engineered Trimeric single-chain
Fv Antibody Fragment○Takashi Tadokoro (Faculty of Pharmaceutical Sciences, Hokkaido University), Kota Nakamura(Faculty of Pharmaceutical Sciences, Hokkaido University), Asami Tomita (Faculty of Pharmaceutical
Sciences, Hokkaido University), Harumi Tsuboi (Faculty of Pharmaceutical Sciences, Hokkaido University),
Reo Ohmura (Faculty of Pharmaceutical Sciences, Hokkaido University), Katsumi Maenaka (Facultyof Pharmaceutical Sciences, Hokkaido University)50

P5-31 Development Of Highly Sensitive Anti-Human Hemoglobin Sensing
Elements Using A Universal Fabrication Technology For Antibody-Enzyme
Complex○Daimei Miura (Tokyo University of Agriculture and Technology), Hayato Kimura (Tokyo University ofAgriculture and Technology), Wakako Tsugawa (Tokyo University of Agriculture and Technology),
Kazunori Ikebukuro (Tokyo University of Agriculture and Technology), Koji Sode (Joint Department of
Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University),

Ryutaro Asano (Tokyo University of Agriculture and Technology)

P5-32 Cyclic ScFv : A Low Molecular Weight Antibody With Reduced Aggregation
Propensity For Biomedical Use○Soichiro Yamauchi (Graduate School of Pharmaceutical Science, Kumamoto University), Chenjiang
Liu (Graduate School of Pharmaceutical Science, Kumamoto University), Natsuki Fukuda (Graduate
School of Pharmaceutical Science, Kumamoto University), Takashi Sato (Graduate School of
Pharmaceutical Science, Kumamoto University), Yoshihiro Kobashigawa (Graduate School of
Pharmaceutical Science, Kumamoto University), Hiroshi Morioka (Graduate School of Pharmaceutical
Science, Kumamoto University)

P5-33 Machine-learning-guided mutagenesis for the directed evolution offunctional proteins
Yutaka Saito (National Institute of Advanced Industrial Science and Technology / RIKEN), Misaki
Oikawa (Tohoku University), Hikaru Nakazawa (Tohoku University), Takumi Sato (Tohoku
University), Tomoshi Kameda (National Institute of Advanced Industrial Science and Technology / RIKEN),
Koji Tsuda (The University of Tokyo / RIKEN), ○Mitsuo Umetsu (Tohoku University / RIKEN)

P5-34 Next-generation sequencing analysis in the directed evolution of antibody
mimics○Tomoyuki Ito (Grad. Sch. Eng., Tohoku Univ.), Hafumi Nishi (Grad. Sch. Information Sci., Tohoku
Univ. / Fac. Core Res., Ochanomizu Univ.), Thuy Duong Nguyen (AIRC, AIST), Yutaka Saito (AIRC,AIST / Adv. Intell. Pro., RIKEN), Tomoshi Kameda (AIRC, AIST / Adv. Intell. Pro., RIKEN), Hikaru
Nakazawa (Grad. Sch. Eng., Tohoku Univ.), Koji Tsuda (Adv. Intell. Pro., RIKEN / Grad. Sch. FrontierSci., The Univ. of Tokyo), Mitsuo Umetsu (Grad. Sch. Eng., Tohoku Univ. / Adv. Intell. Pro., RIKEN)

P5-35 Next-Generation Sequencing Analysis in the Directed Evolution of
Antibodies○Sakiya Kawada (Grad. Sch. Eng., Tohoku Univ.), Yoichi Kurumida (AIRC, AIST), Tomoyuki Ito(Grad. Sch. Eng., Tohoku Univ.), Hikaru Nakazawa (Grad. Sch. Eng., Tohoku Univ.), Hafumi Nishi(Grad. Sch. Information Sci., Tohoku Univ. / Fac. Core Res., Ochanomizu Univ.), Yutaka Saito (AIRC, AIST
/ Adv. Intell. Pro., RIKEN), Tomoshi Kameda (AIRC, AIST / Adv. Intell. Pro., RIKEN), Koji Tsuda (Adv.
Intell. Pro., RIKEN / Grad. Sch. Frontier Sci., The Univ. of Tokyo), Mitsuo Umetsu (Grad. Sch. Eng., Tohoku
Univ. / Adv. Intell. Pro., RIKEN)

51

P5-36 Universal fabrication technology of antibody-enzyme complexes assensing elements using Catcher/Tag systems
Hayato Kimura (Tokyo University of Agriculture and Technology), ○Ryutaro Asano (Tokyo Universityof Agriculture and Technology), Wakako Tsugawa (Tokyo University of Agriculture and Technology),
Kazunori Ikebukuro (Tokyo University of Agriculture and Technology), Koji Sode (Joint Department of
Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University)

P5-37 Development Of Novel Quantitative Method To Evaluate Cross-linking
Ability Of Bispecific Antibodies Using Atomic Force Microscopy○ Atsushi Maejima (Tokyo University of Agriculture and Technology), Kenta Ishibashi (Tokyo
University of Agriculture and Technology / AIST), Hyonchol Kim (Tokyo University of Agriculture andTechnology / AIST), Kazunori Ikebukuro (Tokyo University of Agriculture and Technology), Izumi
Kumagai (Tokyo University of Agriculture and Technology), Ryutaro Asano (Tokyo University ofAgriculture and Technology)

P5-38 Proteomics Approach to Examine Irreversible Proteome Reorganization in
E.coli○Haruka Onodera (School of Life Science and Technology, Tokyo Institute of Technology), Yuhei
Chadani (Cell Biology Center, Tokyo Institute of Technology), Tatsuya Niwa (Cell Biology Center, Tokyo
Institute of Technology / School of Life Science and Technology, Tokyo Institute of Technology), Hideki
Taguchi (Cell Biology Center, Tokyo Institute of Technology / School of Life Science and Technology, Tokyo
Institute of Technology)

P5-39 CF-PPiD: A Cell-Free Human Protein Array Technology and Proximity
Biotinylation-Based Protein-Protein Interaction Identification○Shusei Sugiyama (CellFree Sciences Co., Ltd), Ryo Morishita (CellFree Sciences Co., Ltd),
Miwako Denda (CellFree Sciences Co., Ltd), Satoshi Ozawa (CellFree Sciences Co., Ltd), Satoshi
Yamanaka (Proteo-Science Center, Ehime University), Koki Kido (Proteo-Science Center, Ehime
University), Shogo Nakano (Graduate Division of Nutritional and Environmental Sciences, University of
Shizuoka), Sohei Ito (Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka),
Tatsuya Sawasaki (Proteo-Science Center, Ehime University)

P5-40 Location of Temperature Sensor in the Small Heat Shock Proteins ofMethanogenic Archaea○Rio Midorikawa (Department of Biotechnology and Life Science, Tokyo University of Agriculture andTechnology), Arisa Kannno (Department of Biotechnology and Life Science, Tokyo University of Agriculture
and Technology), Masafumi Yohda (Department of Biotechnology and Life Science, Tokyo University ofAgriculture and Technology)52

P5-41 Comprehensive analysis of affinity maturation of camelid derived single
domain VHH antibody through immunization
Tomonari Matsuda (Research Center for Environmental Quality Management, Kyoto University), Yoko
Akazawa-Ogawa (National Institute of Advanced Industrial Science and Technology (AIST)), LilianKaede Komaba (National Institute of Advanced Industrial Science and Technology (AIST)), Norihiko
Kiyose (ARK Resource. Co., Ltd.), Nobuo Miyazaki (ARK Resource. Co., Ltd.), Yusaku Mizuguchi(JSR Corporation), Tetsuo Fukuta (JSR Corporation), Yuji Ito (Graduate School of Science andEngineering, Kagoshima University), ○Yoshihisa Hagihara (National Institute of Advanced Industrial
Science and Technology (AIST))

P5-42 CodonAdjust: A software for in silico design of a mutagenesis library withspecific amino acid profiles○Thuy Duong Nguyen (Artificial Intelligence Research Center, National Institute of Advanced Industrial
Science and Technology (AIST)), Yutaka Saito (Artificial Intelligence Research Center, National Institute ofAdvanced Industrial Science and Technology (AIST) / AIST-Waseda University Computational Bio Big-Data
Open Innovation Laboratory (CBBD-OIL) / Graduate School of Frontier Sciences, University of Tokyo)

Tomoshi Kameda (Artificial Intelligence Research Center, National Institute of Advanced Industrial Scienceand Technology (AIST))

P5-43 Rotary Properties of Hybrid F1-ATPases Consisting of Subunits fromDifferent Species○Ryo Watanabe (The University of Tokyo), Mariel Zarco-Zavala (The University of Tokyo), ChunBiu Li (Stockholm University, Sweden), Toshiharu Suzuki (Tokyo Institute of Technology), Ryohei
Kobayashi (The University of Tokyo), Hiroshi Ueno (The University of Tokyo), Hiroyuki Noji (The
University of Tokyo)P5-44 Rational Design of Small Proteins that Inhibit a Transcriptional ActivatorCoactivator Interaction○Shunji Suetaka (Department of Life Sciences, The University of Tokyo), Yoshiki Oka (Departmentof Life Sciences, The University of Tokyo), Tomoko Kunihara (Department of Life Sciences, The Universityof Tokyo), Yuuki Hayashi (Department of Life Sciences, The University of Tokyo), Munehito Arai
(Department of Life Sciences, The University of Tokyo / Department of Physics, The University of Tokyo)P5-45 Rational Design of an Inhibitor for the Interaction of the KIX Domain of CBP
with Transcriptional Activators○Nao Sato (Department of Life Sciences, The University of Tokyo), Shunji Suetaka (Department ofLife Sciences, The University of Tokyo), Yuuki Hayashi (Department of Life Sciences, The University ofTokyo), Munehito Arai (Department of Life Sciences, The University of Tokyo / Department of Physics, The
University of Tokyo)

53

P5-46 Directed Evolution of Dimethylallyl Alcohol Kinases○ Yumi Onozato (Chiba University), Naomasa Araki (Chiba University), Shigeko Kawai(Noma) (Chiba University), Daisuke Umeno (Chiba University)

P5-47 Blurring the Genetic Code for the Expansion of Cellular Protein Functions○ Masakazu Araki (Chiba University), Soji Watanabe (Chiba University), Shigeko Kawai(Noma) (Chiba University), Daisuke Umeno (Chiba University)

P5-48 Characterization of a TNFR2-selective Agonistic TNF-α Mutant Protein as
Regulatory T-cell Expander○Masaki Inoue (Kobe Gakuin University / National Institutes of Biomedical Innovation, Health andNutrition), Haruhiko Kamada ( National Institutes of Biomedical Innovation, Health and Nutrition), Shinichi Tsunoda (Kobe Gakuin University / National Institutes of Biomedical Innovation, Health and Nutrition)

P5-49 Creation and evaluation of immunosensors that enable ratiometric assay
by rapid dual-color labeling of antibody fragments○Takanobu Yasuda (Life Sci., Tokyo Tech.), Akihito Inoue (Life Sci., Tokyo Tech.), Tetsuya
Kitaguchi (Res. Inst., Tokyo Tech.), Hiroshi Ueda (Res. Inst., Tokyo Tech.)P5-50 Rational Design Of An Artificial Liquid-liquid Phase Separation System Via
Side-chain Modification Of Synthetic Polypeptide As IDP Mimic For
Effective Protein Sequestration○ Biplab K. C. (Graduate School of Systems Life Sciences, Kyushu University), Takeshi Mori
(Department of Applied Chemistry, Faculty of Engineering, Kyushu University / Center for Future Chemistry,
Kyushu University), Yoshiki Katayama (Department of Applied Chemistry, Faculty of Engineering, Kyushu
University / Center for Future Chemistry, Kyushu University / Center for Molecular Systems, Kyushu University/ Center for Advanced Medical Innovation, Kyushu University), Akihiro Kishimura (Department of Applied
Chemistry, Faculty of Engineering, Kyushu University / Center for Molecular Systems, Kyushu University)

P5-51 Rational Stabilization of Dimeric Four-Helix Bundle De Novo Proteins Using
Molecular Dynamics Simulation○Shin Irumagawa (Shinshu University), Kaito Kobayashi (National Institute of Advanced Industrial
Science and Technology (AIST)), Yutaka Saito (National Institute of Advanced Industrial Science andTechnology (AIST)), Takeshi Miyata (Kagoshima University), Mitsuo Umetsu (Tohoku University),
Tomoshi Kameda (National Institute of Advanced Industrial Science and Technology (AIST)), Ryoichi
Arai (Shinshu University)54