Process Chromatography

Process Resin Solutions
for Vaccines and Therapeutics
Bio-Rad offers a wide range of chromatography resins for the purification of viruses
and viral particles for applications in vaccine development and disease prevention
and for use as delivery mechanisms in gene therapy. Examples of workflows arelisted in Tables 1 and 2. Virus purification results are shown in Figure 1.
Table 1. Resins for virus purification.
Application

Product

Bulletin

Single-step mammalian virus purification

CHT Mixed Mode6790

Purification of viruses and virus-like particles

Nuvia Q7200

Development of an adenovirus manufacturing process

Nuvia cPrime, Nuvia Q

6719

Swine influenza A H1N1 purification

Nuvia HP-Q

7375

Visit bio-rad.com/ResinSolutions for more information.

Process Resin Solutions for Vaccines and Therapeutics

Table 2. Wide variety of viruses that can be purified using CHT Media.
Virus

FamilyGenus

GenomeEnvelope

Size, nmDengueFlaviviridae

Flavivirus

ssRNA+50

Japanese encephalitis

Flaviviridae

Flavivirus

ssRNA+

50

Influenza

Orthomyxoviridae

Influenzavirus

ssRNA+80–120

Mouse hepatitis

Coronaviridae

Coronavirus

ssRNA+

100–150

Adenovirus

Adenoviridae

Mastadenovirus

ssRNA–90

Poliovirus

Picornaviridae

Enterovirus

ssRNA

–

30

Feline calicivirus

Caliciviridae

Vesivirus

ssRNA–30–38

Refer to bulletin 6790 for more information.

Bioprocess resins for purification of therapeutics are shown in Table 3. Bio-Rad offers unique process chromatography resins,
which provide purification benefits spanning a range of biomolecules. Choose from new selectivity and high-capacity chromatography
techniques to maximize overall process efficiencies for your therapeutic.
B

1000.88.0 x 105

0.6

6.0 x 105

0.4

4.0x 105

0.240
1,500

1,0005000510152025
Elution volume, ml

303540020102.0 x 105

030001.2

1.2 x 104

1.01.0 x 104

0.8

8.0 x 103

0.6

6.0 x 103

0.4

4.0 x 1030.2

2.0 x 103

00510152025
Elution volume, ml

30

3540

2,000

1,500

1,000dsDNA, ng/ml

1.0 x 10 6Absorbance

1.050

2,000Conductivity, mS/cm

20

1.2 x 10 6dsDNA, ng/ml

30

Absorbance

Conductivity, mS/cm

40

1.2Infectivity, TCID50 /ml

50Infectivity, TCID50 /ml

A500

00Fig. 1. Chromatography of two strains of mouse hepatitis virus (MHV), a coronavirus (CoV) that includes SARS-CoV. A, MHV-NuU; B, MHV-S. UV absorbance at260 nm (—); UV absorbance at 280 nm (—); conductivity (—); viral infectivity in TCID50 (—); dsDNA (—). Culture fluid contained 10% fetal bovine serum. Refer to bulletin 6549.
TCID50, 50% tissue culture infective dose.
Table 3. Resins for purification of therapeutics.
Application

ProductBulletin

Efficient antibody purification with novel selectivities

Nuvia Q, Nuvia S, Nuvia HR-S, Nuvia cPrime, CHT,
UNOsphere SUPrA, UNOsphere Q

6889

Achieve higher yield and purity with sharper monoclonal antibody elution profiles

Nuvia HR-S

7000

Purification of large biomolecules with a high-capacity ion exchanger

Nuvia HP-Q

7127

Reference
Kurosawa Y et al. (2014). Mammalian virus purification using ceramic hydroxyapatite. Bio-Rad Bulletin 6549.
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