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BIOSCIENCECATALOGUEEDITION 2024

To us, health is the highest personal priority.
This inspires our daily mission of serving life sciences
and healthcare professionals with advanced andsustainable products and solutions.
It is the passion and commitment of our employees
employees that makes the difference.
With a targeted approach, our global teamcontinuously strives for excellence
and works closely with our customers.
We empower people to make a difference in health.

CONTENT
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6

10

12

14

PRODUCTS

BIOSCIENCE

PREANALYTICS

OEM

Here you can findour productoverview

Making a difference in researchand science

Preanalyticalspecimen collection systems andsafety solutions

Tailor madesolutions

17

18

SERVICEVALUEPROPOSITIONS

E-Commerce
Solutions from
Greiner Bio-One

251 284

TECHNICALAPPENDIXNUMERICAL
INDEX

About our value
propositions

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PRODUCTS

35

77
89
105

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CELL CULTURE
CELLSTAR® Cell Culture Flasks 
CELLSTAR® Cell Culture Dishes 
CELLSTAR® Cell Culture Multiwell Plates 
ThinCert® Cell Culture Inserts for 6, 12 and24 Well Multiwell Plates 
ThinCert® 96 Well HTS Insert for
high-throughput applications 
CELLSTAR® Cell Culture Microplates 
CELLSTAR ® Cell Culture Tubes 
EASYstrainer Cell Strainers 

40
48
53
57
62
64
72
74

MASS CELL CULTURE
CELLMASTER Cell Culture Roller Bottles 
CELLdisc Cell Culture Device 

78
81

3D CELL CULTURE
CELLSTAR ® Cell Culture Vessels Cell-Repellent Surface
Magnetic 3D Cell Culture 
ThinCert® Cell Culture Inserts for 6, 12
and 24 Well Multiwell Plates 

 90
94
99

PRODUCTS FOR MICROSCOPY
CELLview Dish Cell Culture Dish with Glass Bottom 
CELLview Slide Cell Culture Slide with Glass Bottom 
CELLview Plate Cell Culture Plate with Glass Bottom 
SCREENSTAR Microplates 
SensoPlate Glass Bottom Microplates 

110
112
114
116
118

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Online Shop121

HTS-MICROPLATES
96 Well Microplates 
384 Well Microplates 
1536 Well Microplates 
Polypropylene Storage Plates Microplates for Compound Storage
Non-binding Microplates Streptavidin-coated Microplates 
UV-Star® Microplates 
Lids / Sealers / CapMats Protein Crystallisation Plates 155

IMMUNOLOGY / HLA

171

MICROBIOLOGY179

TUBES / MULTIPURPOSE BEAKERSELISA Microplates
ELISA Strip Plates
Immuno Tubes 
Terasaki Plates 

160
162
165
167




Dishes / Plates / Others

126
130
134
136
139
141
144
146
148
151

172



Tubes without closures Tubes with closures 
Separation Tubes 
Multipurpose Containers / Beakers



180
183
188
190

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PRODUCTS

195
217

CRYOTECHNICS
Cryo.s Cryo Tubes 
Cryo.s with Barcode 
Cryo.s Biobanking Tubes

196
202
205



REACTION TUBES / ANALYSER
CUPS
218
221

Reaction Tubes Analyser Cups Semi-micro / Macro Cuvette 

225 MOLECULAR BIOLOGY
Sapphire PCR Tubes 
Sapphire PCR Microplates 

233 LIQUID HANDLING

Sapphire Pipettes 
Sapphire Pipette Tips 
CELLSTAR® Serological Pipettes251

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234
237
246

LAB EQUIPMENTLab Equipment



257 TECHNICAL APPENDIX

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226
230

252

WE ARE PROUD TO PRESENT YOU OUR NEW
DIGITAL SHOWROOM & 3D CELL CULTURE LAB

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BIOSCIENCE
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MAKING A DIFFERENCE IN RESEARCH
AND SCIENCE
Greiner Bio-One is one of theleading suppliers of special
products for the cultivation andanalysis of cell and tissue cultures. Under the brand nameCELLSTAR®, we offer cell culture flasks, dishes and plates
in a wide variety of formats andsurface modifications, so thatyou will always find exactly theright product for every application and all cell types.
In addition, we have been developing and producing microplates
for high-throughput screening
for more than 60 years, enablingindustry and research to carryout fast and efficient drug testing. Among other things, Greiner
Bio-One was the first manufacturer to introduce a microplate in
1536 well format to the market,
setting new standards in termsof automation, performance andcost reduction.
Based on decades of experiencein the cryogenic storage of samples, we also offer comprehensive solutions for automatedstorage systems in biobanks.

/ High-quality products for your lab/ Special products for cell and tissue culture/ Microplates for industry and research/ Cryogenic storage systems

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PREANALYTICS
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PREANALYTICAL SPECIMENCOLLECTION SYSTEMS AND SAFETY
SOLUTIONS
Greiner Bio-One was the firstcompany in the world to successfully launch a blood collection system made out of virtuallyunbreakable PET plastic including a safety cap. Many components of the VACUETTE® System have often been copied, butnever reached our high quality
standards.
As a trendsetter in the fieldof sample collection, we offer
solutions that meet the highest demands of our customers.
Because your work is an important contribution to healthprotection.
Continuously new and improved
product solutions, perfect functionality, maximum safety andhigh product quality are the result of decades of experience.

/ Provide safety/ Simplify handling/ Provide support/ Increase efficiency/ Save costswww.gbo.com

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OEM
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TAILOR MADE SOLUTIONSGreiner Bio-One as an OriginalEquipment Manufacturer (OEM)is a long-term partner of thepharmaceutical industry, biotechnology, diagnostic and medical technology industries. The
company manufactures numerous products through injection
moulding and offers the entire
product development and production process. From design,

via prototyping, through fully
automated manufacturing,
Greiner Bio-One delivers thecomplete solution with supportof experienced scientists, engineers and specialists.
With a global sales network specifically established for the fieldof OEM, Greiner Bio-One offers
custom-made solutions withpersonal service.

/ Research & development with diverse application and researchlaboratories/ Construction of moulds, planning of plants and assembly lines/ Modern, fully automated production/ Post-production according to customer requirements/ Comprehensive quality assurance and management system/ Worldwide networked warehousing and customer service

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SERVICE@
GREINER
BIO-ONE

FAST. SECURE. EFFICIENT.
E-COMMERCE SOLUTIONS FROM
GREINER BIO-ONE
As your strong partner in thefields of biotechnology, diagnostics, medical devices and research, Greiner Bio-One alreadyprovides you with solutions ona daily basis. In addition, we
can offer you a wide selectionof e-business solutions to ensure the optimal integration of
our products and services into
your purchasing process. Thatin turn will significantly reduce
your process-related costs andwarehouse costs.
Take advantage of e-businessconnectivity and make your processes faster, more secure andmore efficient.

Greiner Bio-One offers you numerous solutions and the necessary expertise to support yourprocesses, regardless of which
ERP system you use. Would you
like to purchase via our online
shop or are you interested in oneof our eProcurement solutions
such as EDI or OCI?
In order to stay informed, you
can subscribe to our Greiner
Bio-One Newsletter and nevermiss any news about our products and events.

/ Online-Shop/ EDI (Electronic Data
Interchange)/ OCI (Open Catalogue
Interface)/ Electronic
catalogues

Subscribe to our newsletterand never missthe latest news!

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As scientists, weshare your passion foryour research success.
We are happy to support you
wherever and whenever
we are needed.

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TRUE TRUSTTake advantage of
our experience andpassion for yourprojects and tasks

WE KEEP OUR PROMISESFor us, real trust means keeping promises. This is one of thecore values that we are committed to as a company, both
towards you as our customers
and towards our employees.
As a family-owned company,
we believe that trusting, active
partnership is the key to sustainable and reliable processes. We

understand your requirements,
speak your language and are
happy to support you in advancing your scientific progress. We
are at your side at every stage –our competent team of experts
is always on hand, from the firstcontact and flawless deliveryof our products, to all forms ofafter-sales service.

PUT YOUR TRUSTIN US:/ Partner for sustainable and reliable
processes/ 60 years of experience in science andhealthcare/ Innovation withtradition/ Support during
every work step

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MORE:

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With state-of-the-art technology,
we develop high-performancesolutions for consistent,
reproducible results thatyou can always rely on.

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QUALITY
Reproducible results
you can rely on

CONSISTENTLY HIGH QUALITY FORRELIABLE RESULTS

Quality has been our top priority for 60 years. This applies all over the world, as allof our production facilities arecommitted to the same highstandard.
Our products are made fromstrictly controlled, certified raw
materials. With state-of-theart production technology andcontinuous in-process checks
by specially trained employees,
we ensure consistent quality ofthe products. Quality promise
not only relates to the manufacturing process and therefore to
product quality, but to every process and every interaction withyou. We are ISO 9001 and ISO
13485 certified.

CONSISTENT HIGHQUALITY:/ Locationindependent/ State-of-the-art
manufacturing
technologies/ Controlled andcertified raw
materials/ ISO 9001 and ISO
13485 certified

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MORE:

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Thanks to our presence in over 100
countries, you can rely on us toprovide you with consistent highquality, availability and support.
Wherever you are.

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GLOBALPRESENCE
There to support you- wherever you are

ON-SITE FOR YOU WORLDWIDEWE PLACE IMPORTANCE ON:
By global presence we mean
being there for you whenever
and wherever you need us.
Our seven production sites in
Europe, Asia and North andSouth America meet the samehigh quality standards andensure low-risk delivery routes
and a secure product supply.
The support teams of our 28 offices and distributors in morethan 100 countries are helpful
and competent, and are always
on hand when you need help or
support.

/ Competent local
support/ Optimum productavailability/ Consistent highquality worldwide

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Our innovative spirit
supports the results ofyour pioneering work.
In the past, present
and future.

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STATEOF-THE-ART
TECHNOLOGY
Pioneers for
progress

IDEAS BECOME SOLUTIONS

For more than 60 years, ourpassion for plastic technology
and manufacturing processes
has driven us to constantly innovate to support you optimally in your daily work and ensure
your success.
We have been instrumental in
the development of consumables in the diagnostics and life
sciences industries, as well as in
the medical market, since 1963.

We are proud of our rich pioneering history – from the productionof the first plastic petri dishes,to Europe’s first plastic vacuum
sampling system or the invention of the world’s first 1536-well
microplates. We will continue
to strive for new and innovative
product solutions that are precisely tailored to your requirements and optimally support you
in your daily work.

PROGRESS NEEDSPIONEERS:/ Product solutions
for current andemerging trends/ Innovative production technologies

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We work hand in hand with you todevelop tailored solutions to suit
your requirements.

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CUSTOMER
FOCUS
We focus on you andyour goals

WE SUPPORT YOU SO WE CAN MAKE
BIG THINGS HAPPEN TOGETHER

Everything we do and the way
we do it is designed to makeyour work easier. By understanding your wishes andneeds, as well as your requirements and workflows, we areable to offer you real added
value and thus trust in longterm and sustainable
customer relationships.
We are here to provide you withthe best products and solutions

to suit your needs. Our dedicated team ensures that processes
run smoothly and is always on
hand when needed. Our multidisciplinary teams have many years
of practical experience in science and laboratory work andunderstand the challenges ofyour daily work first hand. We are
happy to use this expert knowledge to create training materials
tailored to your specific needs.

WE OFFER:/ Long-term customer relationship/ Solutions to suit
your requirements/ Training and training materials

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We use over 60 years of experiencein the laboratory and healthcare
sectors, and our passion
for innovation, to meet yourrequirements and grow with you.

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EXPERTISE
In all relevant market
trendsWE SPEAK YOUR LANGUAGE

We understand your tasks andtheir workflows with all theirrequirements. Our experts areat your side with more than
60 years of experience – fromthe development of products
and solutions to support withregulatory issues or individual
training.
More than 80% of our sales andapplication specialists have abackground in natural sciences

and practical experience in thelaboratory. We would be happy toadvise you on site – at the place
where your requirements arise.
You can rely on our expertise toquickly integrate our products
into your processes, because
we know and understand yourworkflow. We are happy to support you in every step of your individual workflow, for example
with product training, support
documentation and service.

WHAT WE OFFER:/ Comprehensive
portfolio for yourworkflow needs/ Experienced teamof industry experts/ Best practices for
optimal efficiency

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We help you to optimize youroperations and processes so thatyou can always achieve the best
possible results.

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EFFICIENCY
We are your partner
for increasing
efficiency – insideand outside thelaboratory

WE INCREASE YOUR EFFICIENCY

In the laboratory and day-today work, increasing efficiency is no longer just an option,
but an essential requirement.
As a strategic cooperation
partner for day-to-day routine
activities in and outside thelaboratory, we support you in
overcoming your current andfuture challenges.
We offer significant added value by supporting the efficien-

cy of your workflows. For example, our digital solutions create
a connection between pre-barcoded test tubes and patient
data from the laboratory information system. This reliably
rules out mix-ups and minimizes sampling errors. Your workflows can thus be measurably
optimized, better results can beachieved with minimum time, resources, and work.

YOUR STRATEGICPARTNER:/ Increased efficiency/ Digital solutions/ Best practicerecommendations

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We are committed to climate
protection and limiting global
warming to 1.5°C.

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SUSTAINABILITY
the principle behind
our processes formany years

BECAUSE IT’S REALLYIMPORTANT TO US

Sustainable action is the basic
prerequisite for a future worth
living in. As a plastics processing company, we strive to develop sustainable solutions
that provide answers to themajor challenges of our time.
Greiner AG’s overarching “BluePlan” strategy focuses on thethree pillars of climate, circular economy and people. Greiner

Bio-One is also working intensively on these sub-areas.
As a producer of plastic medical
products, it is our declared longterm goal to combine ecological,
economic and social aspects.
We attach particular importance not only to your excellent
supply of high-quality products,
but also to energy-efficient andsustainable operation as a plastics producer.

OUR GOALS UNTIL
2030:/ Comprehensive
circular approach/ Preparation of
employees for thechallenges of the
futureDISCOVER
MORE:

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The secret of the continuing success of the CELLSTAR® productline from Greiner Bio-One lies in its high quality and reliability. For
more than 60 years, Greiner Bio-One has been setting standards for
future-oriented research and new technologies in the field of cell
culture.

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Cell Culture﻿

CELL CULTURE/ CELLSTAR® Cell Culture Flasks ����������� 40

ThinCert® Plate 6 / 12 Well��������������������������������60

CELLSTAR ® Standard Cell Culture Flasks �������� 41
Cell Culture Flasks - CELLCOAT® ���������������������42/ ThinCert ® 96 Well HTS Insert For highthroughput applications����������������������� 62

Suspension Culture Flasks ������������������������������43

ThinCert ® 96 Well HTS Insert (Membrane plates

Cell Culture Flasks - Advanced TC �������������������42

Cell Culture Flasks Cell-Repellent Surface������44
AutoFlask - Cell Culture Flask For AutomatedSystems������������������������������������������������������44

and receiver plates)�������������������������������������63
Receiver plates for ThinCert ® 96 Well HTS Insert��������������������������������������������������������������������63

Cell Scraper ����������������������������������������������������45
Media Bottles ��������������������������������������������������46/ CELLSTAR® Cell Culture Microplates���� 64

Disposal Bags ������������������������������������������������� 47

Cell Culture Microplates 96 Well�����������������������65
Cell Culture Microplates 96 Well - µClear®��������66/ CELLSTAR® Cell Culture Dishes ����������� 48

Cell Culture Microplates 96 Well - Half Area�����66

Cell Culture Dishes ������������������������������������������49

Cell Culture Microplates 96 Well - CELLCOAT®�67

Cell Culture Dishes - Advanced TC ������������������49

Cell Culture Microplates 96 Well - Advanced TC6 8

Cell Culture Dishes - CELLCOAT® ��������������������50

Suspension Culture Microplates 96 Well����������68

Cell Culture Dishes Cell-Repellent Surface������ 51

Cell Culture Microplates 384 Well���������������������69

CELLview Dish ������������������������������������������������ 51

Cell Culture Microplates 384 Well - Small Volume

OneWell Plate FourWell Plate���������������������������52��������������������������������������������������������������������70
Cell Culture Microplates 1536 Well��������������������70/ CELLSTAR® Cell Culture Multiwell Plates������������������������������������������������������������ 53

Lids ����������������������������������������������������������������� 71

Multiwell Plates 6 / 12 / 24 / 48 Well Format������54/ CELLSTAR ® Cell Culture Tubes ������������� 72

Multiwell Plates Advanced TC��������������������������54

CELLreactor ���������������������������������������������������73

Multiwell Plates CELLCOAT®����������������������������55

CELLSTAR® Cell Culture Tubes������������������������73

Multiwell Plates Cell-Repellent Surface�����������56/ EASYstrainer Cell Strainers ������������������74/ ThinCert® Cell Culture Inserts for 6, 12 and24 Well Multiwell Plates������������������������ 57

EASYstrainer ��������������������������������������������������75

ThinCert® Cell Culture Inserts 6 Well����������������58
ThinCert® Cell Culture Inserts 12 Well��������������59
ThinCert® Cell Culture Inserts 24 Well��������������60

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Cell Culture﻿

CELLSTAR®
Quality and reliability
for over 60 years/ Automated production
processes/ Consistent quality control
system/ High-quality raw material

Vents for optimal gas exchangeHigh transparency

Easy stacking

Standard screw cap
with ventilation position

Canted neckGraduation andwriting area on both sides

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Cell Culture﻿

OPTIMISED CELL CULTURE SURFACES
FOR BETTER RESULTS
Cells cultured on plastic surfaces require interaction withmolecules in the microenvironment to stimulate normal functioning and proliferation. Easily
culturable cell lines are commonly grown on tissue culture treated surfaces like CELLSTAR®
tissue culture or in suspension
like CELLSTAR® suspension
culture. The CELLSTAR® product line offers solutions formost applications in cell biology
including the propagation ofcells, the performance of cellbased assays, and imaging
procedures.

CELLSTAR® cell culture vessels
with a cell-repellent surface reliably prevent cell attachment
in suspension cultures of semiadherent and adherent cell lines
where standard hydrophobic
surfaces generally used for suspension culture are insufficient.
All cell culture products arelabelled with expiry date and lot
number in order to ensure transparency of product processes
and retraceability of our products throughout the production
process.

LITERATURE:/ Application Note
„siRNA-dependent
gene silencing on
various cell culture
surfaces” (F071105)/ Application Note
„Improved cultivation / differentiationof embryonic stem
cells“ (F073117)/ Application Note
„Cultivation anddifferentiationof hADSCs withCELLSTAR® and
CELLCOAT®
products“ (F073113)

QUALITY CONTROL
Based on automatedproduction processes
with quality controls,
we provide immaculate,high-quality products
for all areas of cell
culture.

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Cell Culture﻿

THE BEST SCREW CAP FOR EVERY APPLICATIONThe secured click-in ventilation position isreached, once the cap snaps in audibly. In
addition, the correct position is indicatedby a vertical tip of a triangle and the readable writing VENT. This allows visual verification of the aeration position, even when
the flasks are stacked in the incubator. If
the cap is turned clockwise to the end stop,
the flask is closed gas-tight.
Filter screw caps for cell culture / suspension culture flasks, roller bottles and the
CELLreactor have a patented hydrophobic
capillary pore membrane. The defined andconstant pore size of 0.2 μm is achieved
with minimal variation by means of a specially developed, high-technology method.

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The filter insert provides both optimal
protection against contamination andefficient gas exchange. By using PET /PTFE which are responsible for the mechanical strength and hydrophobic properties of the membrane, these advantages are retained even if the insideof thecap is briefly wetted with medium.

Cell Culture﻿

HIGH-QUALITY RAW
MATERIAL
Exclusively high-grade
polystyrene and polyethylene
terephthalate are used as raw
materials for manufacturing ourcell culture products.

Polystyrene (PS) is characterised by its high clarity,
which greatly simplifies the
optical control of cell growthin polystyrene flasks, tubes
and roller bottles.

Polyethyleneterephthalate(PET) is used for manufacturing roller bottles, media bottles and membranes,
due to its beneficial chemical, optical and mechanicalproperties.

CELLSTAR®

Advanced TC

CELLCOAT®

CELLSTAR® TC is the standard surface for cultivation ofadherent cells. The special
physical surface treatment
leads to the incorporation ofpolar groups such a carboxyl
and hydroxyl residues, whichfunctionalised the hydrophobic polystyrene surface
resulting in improved andconsistent cell attachment.

The Advanced TC surface
is based on polymer modification to result in a highlyhydrophilic surface which
positively influences cellularfeatures and functions.
Advanced TC facilitatesconsistent and homogenous
cell attachment, increasing
the overall cell yield andreducing cell loss.

The CELLCOAT® productline comprises cell culture
vessels which are coatedwith biological or synthetic
proteins of the extracellularmatrix (Collagen Type I,
Fibronectin, Laminin) or
synthetic proteins (Poly-DLysine, Poly-L-Lysine).

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Cell Culture
CELLSTAR® Cell Culture Flasks/

This video shows how
much fun you can havewith our cell culture
flasks in your lab:/

Standard or filter
screw cap/

Canted neck/

Sterile and userfriendly packaging/

Growth area foradherent cells: 25, 75
and 175 cm2/

Filling volume forsuspension cells: 50,
250, 550, 650 ml

CELLSTAR® CELL CULTURE FLASKSGreiner Bio-One offers standard and filter cap cell culture
flasks with different surfaces.
All Greiner Bio-One cell culture
flasks are made of high-grade
polystyrene and are free of detectable DNases, RNases, human DNA, endotoxins and are
non-cytotoxic.
The specific design facilitates
optimal access to the flask whenusing a cell scraper or pipette.
The top stacking rim stabilizes the flask and facilitates easy
flask stacking in the incubator.

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Both sides of the flask have printed graduation marks to aid liquid
fill. Filter or standard screw caps
options available with click-in
ventilation positioning to guarantee gas exchange.
Greiner Bio-One flasks offer a
specific physical surface treatment to ensure adhesion andproliferation of adherent cells.
In contrast, the Advanced TC
surface options provide optimal conditions for the cultivation of sensitive and fastidious
cells as well as for use in restricted growth conditions.

Cell Culture
CELLSTAR® Cell Culture Flasks

CELLSTAR ® Standard Cell Culture Flasks
For the maintenance of adherent cells Greiner
Bio-One offers a specific physical surface
treatment which ensures adhesion andproliferation of these cells.
/

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

Item No. 690170 with measuring grid

nonpyrogenic

Raw material: PS, Surface treatment: TC, Sterile: +Growth area

Cap colour

Working volume

Total
volumeCap design

SterileQty. inner /outer69016025 cm²l red

5 ml - 10 ml

50 ml

screw cap+

10 / 200

69017025 cm²l red

5 ml - 10 ml

50 ml

screw cap+

10 / 200

690160-TRI

25 cm²l red

5 ml - 10 ml

50 ml

screw cap+

10 / 13069017525 cm²l red

5 ml - 10 ml

50 ml

filter screw cap+

10 / 200

690175-TRI

25 cm²l red

5 ml - 10 ml

50 ml

filter screw cap+

10 / 13065817075 cm²l red

15 ml - 38 ml

250 mlscrew cap+

5 / 120658170-TRI

75 cm²l red

15 ml - 38 ml

250 mlscrew cap+

5 / 9065817575 cm²l red

15 ml - 38 ml

250 mlfilter screw cap+

5 / 120658175-TRI

75 cm²l red

15 ml - 38 ml

250 mlfilter screw cap+

5 / 90+5 / 50+5 / 40+5 / 50+5 / 40Item no.

Flask
designTriple-packed

yes
yes
yes
yes

660160flat

175 cm²

l red

20 ml - 45 ml

550 mlscrew cap

660160-TRI

flat

175 cm²

l red

20 ml - 45 ml

550 mlscrew cap

660175flat

175 cm²

l red

20 ml - 45 ml

550 mlfilter screw cap

660175-TRI

flat

175 cm²

l red

20 ml - 45 ml

550 mlfilter screw cap

661160high

175 cm²

l red

20 ml - 85 ml

650 mlscrew cap+

4 / 40661175high

175 cm²

l red

20 ml - 85 ml

650 mlfilter screw cap+

4 / 40yes
yes/ The item no. of triple-packed products is composed of the standard item no. plus –TRI (e.g. 604160-TRI). In general, all Greiner Bio-One cell
culture products can be produced triple-packed. Please contact your Greiner Bio-One sales representative regarding customised triplepacked products.

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Cell Culture
CELLSTAR® Cell Culture Flasks

Cell Culture Flasks - Advanced TC
The Advanced TC surface provides optimal
conditions for the cultivation of sensitive andfastidious cells or the usage of restricted
growth conditions.

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Consistent and even cell attachment/

Homogeneous and optimised cell growth

nonpyrogenic

Raw material: PS, Surface treatment: Advanced TC, Sterile: +Growth area

Cap colour

Working volume

Total volume

Cap design

SterileQty. inner /outer69097525 cm²l blue5 ml - 10 ml

50 ml

filter screw cap+

10 / 200

65897075 cm²l blue15 ml - 38 ml

250 mlscrew cap+

5 / 12065897575 cm²l blue15 ml - 38 ml

250 mlfilter screw cap+

5 / 120

Item no.

Flask design

660960flat

175 cm²

l blue20 ml - 45 ml

550 mlscrew cap+

5 / 50660975flat

175 cm²

l blue20 ml - 45 ml

550 mlfilter screw cap+

5 / 50/ Forum No. 12: Advanced TC: An innovative surface improving cellular assays (F071104)/ Application Report „Advanced TC for improving the cultivation / differentiation of embryonic stem cells“ (F076036)

Cell Culture Flasks - CELLCOAT®
/

Canted neck for optimal access/

Graduation on both sides/

Improved adhesion and cell proliferation/

Reduced-serum or serum-free cultivation/

Improved growth of primary cells

Raw material: PS, Surface treatment: CELLCOAT®, Cap design: filter screw cap

Item no.

Growth area

Protein coating

Cap colour

Working volume

Total volume

Qty. inner / outer

69095025 cm²

Collagen Type I

l red

5 ml - 10 ml

50 ml

10 / 50

65895075 cm²

Collagen Type I

l red

15 ml - 38 ml

250 ml5 / 50175 cm²

Collagen Type I

l red

20 ml - 85 ml

650 ml5 / 4069094025 cm²Poly-D-Lysine

l red

5 ml - 10 ml

50 ml

10 / 5065894075 cm²Poly-D-Lysine

l red

15 ml - 38 ml

250 ml5 / 5066195042 /www.gbo.com

Flask designhighCell Culture
CELLSTAR® Cell Culture Flasks

Item no.

Flask design

Growth area

Protein coating

Cap colour

Working volume

Total volume

Qty. inner / outerhigh

175 cm²

Poly-D-Lysine

l red

20 ml - 85 ml

650 ml5 / 4069092025 cm²

Fibronectinl red

5 ml - 10 ml

50 ml

10 / 1065892075 cm²

Fibronectinl red

15 ml - 38 ml

250 ml10 / 10175 cm²

Fibronectinl red

20 ml - 85 ml

650 ml5/569091025 cm²Lamininl red

5 ml - 10 ml

50 ml

5 / 1065891075 cm²Lamininl red

15 ml - 38 ml

250 ml5 / 10175 cm²

Lamininl red

20 ml - 85 ml

650 ml5/5661940661920high661910high

Suspension Culture Flasks

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Hydrophobic surface, ideal for suspension
cultures, hybridoma and embryonic stem cells/

Graduation on both sides/

Sterile and user-friendly packaging/

Canted neck for optimal accessnonpyrogenic

Raw material: PS, Surface treatment: suspension, Sterile: +Item no.

Flask design

Cap colour

Total volume

Cap design

690190l white50 ml

screw cap

690190-TRI

l white50 ml

screw cap

690195l white

50 ml

filter screw cap

690195-TRI

l white

50 ml

filter screw cap

658190l white

250 mlscrew cap

658190-TRI

l white

250 mlscrew cap

658195l white

250 mlfilter screw cap

658195-TRI

l white

250 mlfilter screw cap

Triple-packed

yes
yes
yes
yes

SterileQty. inner / outer+

10 / 200+

10 / 130+

10 / 200+

10 / 130+

5 / 120+

5 / 90+5 / 120+

5 / 90+5 / 50+5 / 40660190flat

l white

550 mlscrew cap

660190-TRI

flat

l white

550 mlscrew cap

661190highl white

650 mlscrew cap+

4 / 40661195highl white

650 mlfilter screw cap+

4 / 40661195-TRI

highl white

650 mlfilter screw cap+

4 / 28yes

yes

www.gbo.com

/ 43

Cell Culture
CELLSTAR® Cell Culture Flasks

Cell Culture Flasks
Cell-Repellent Surface
A cell-repellent surface reliably prevent cellattachment in suspension cultures ofsemi-adherent and adherent cell lines where
standard hydrophobic surfaces generally used
for suspension culture are insufficient.

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Raw material: PS, Surface treatment: cell-repellent, Sterile: +Item no.

Flask design

Cap colour

Total volume

Cap design

SterileQty. inner / outer

690980flat

l white50 ml

screw cap+

10 / 20690985flat

l white

50 ml

filter screw cap+

10 / 20658980flat

l white

250 mlscrew cap+

5 / 15658985flat

l white

250 mlfilter screw cap+

5 / 15660980flat

l white

550 mlscrew cap+

5/5660985flat

l white

550 mlfilter screw cap+

5/5661980highl white

650 mlscrew cap+

4/4661985highl white

650 mlfilter screw cap+

4/4

AutoFlask - Cell Culture Flask
For Automated Systems

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Standard microplate footprint/

Compatible with a wide range of cell culture andliquid handling systems/

Hydrophobic filter membrane/

Different surface treatments/

Customised barcoding on request

nonpyrogenic

Flask design: AutoFlask, Growth area: 83.6 cm², Raw material: PS, Total volume: 110 ml, Sterile: +Item no.

77916044 /www.gbo.com

BarcodeSurface treatment

yes

TC

Product colour

Colour code

Working volume

SterileQty. inner / outerl clearl red

20 ml - 40 ml+

10 / 100

Cell Culture
CELLSTAR® Cell Culture Flasks

Item no.

779190BarcodeSurface treatment

yessuspension

Product colour

Colour code

Working volume

SterileQty. inner / outerl clearl white

60 ml - 80 ml+

10 / 100/ Further information on the AutoFlask: “Comparative cell growth study using the AutoFlask” (F072094)

Cell Scraper/

For gentle mechanical removal of adherent cells/

Optimised blade design for maximum cell harvest/

Blade length: 1.8 cm/

Pivot angle 60°/

Sterile individual packaging

STERILE

Description: Cell Scraper, Feature: blade length 1.8 cm, Sterile: +Item no.

LengthSterileQty. inner / outer

54107028 cm+

50 / 100

54108040 cm+

100 / 100

www.gbo.com

/ 45

Cell Culture
CELLSTAR® Cell Culture Flasks

Media Bottles

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Made of polyethylene terephthalate (PET)/

Available in three sizes/

With graduation/

Triple-packed for GMP compliant workflow

nonpyrogenic

Flask design: Tetragonal, Raw material: PET, Cap design: screw cap, Sterile: +Item no.

46 /Cap colour

Total volume

SterileQty. inner / outer

950700l white

500 ml+25 / 50

951700l white

100 ml+25 / 100952700l white1,000 ml+

24 / 24www.gbo.com

Cell Culture
CELLSTAR® Cell Culture Flasks

Disposal Bags
Disposal bags made of polypropylene forsterilisation in the steam autoclave. For usersof hot-air sterilisers, disposal bags made ofpolyamide are suitable for use up to +160 °C.

Foil thickness: 0.05 mm
LengthWidthNominalcapacity

Suitable for steam
autoclaves

500 mm

300 mm

10 l

500 mm

300 mm10 l643401500 mm

300 mm10 l644201780 mm

400 mm

30 l

780 mm

400 mm

30 l

780 mm

400 mm

30 l

780 mm

600 mm65 l646203780 mm

600 mm65 l

646401780 mm

600 mm65 l649201

1,100 mm

700 mm130 l

1,100 mm

700 mm130 l

1,100 mm

700 mm130 lItem no.

Feature643201643203644203

Imprint "Biohazard"

Imprint "Biohazard"

644401646201649203649401

Imprint "Biohazard"

Imprint "Biohazard"

Suitablefor hot air
sterilizers

Material

Qty. inner /
outer

yes

PP

500 / 500

yesPP

500 / 500PA

500 / 500

yesPP

500 / 500

yesPP

500 / 500PA

500 / 500

yesPP

500 / 500

yesPP

1 / 500

PA

300 / 300

yesPP

350 / 350

yesPP

350 / 350PA

200 / 200

yes

yes

yes

yes

www.gbo.com

/ 47

Cell Culture
CELLSTAR® Cell Culture Dishes/

For exact dimensionsof our cell culture dishes, please refer to the
product data sheets on
our website.

/

Easy stacking/

Maximal transparency
for excellent
microscopic analysis/

Improved cell adhesion
through physicalsurface treatment

CELLSTAR® CELL CULTURE DISHESOur CELLSTAR® cell culture
dishes are manufactured
to meet the highest quality
standards at Greiner Bio-One.
This assortment of dishes offers a range of growth sizes
from 8.7 to 143 cm². These dimensions are provided in nominal sizes of 35, 60, 100, and 145
mm. For culture dishes associated with growth areas of either
58 or 143 cm², we supply variants
with side heights of 20 mm (forspecial applications). In addition
to our standard formats we offer 35 mm cell culture dishes
that are equipped with four in-48 /www.gbo.com

ner rings. Our standard cell culture dishes have been treatedin order to optimise growth andadhesion.
However, we are also capableof providing sterile cell culture
trays without any type of coating for suspension culture. Similar to all of our cell culture laboratory products, this range ofdishes is packed user-friendly
within sterile conditions. This allows technicians to safely handle their samples with a wide
variety of cell culture methods
without the risk of cell culture
contamination.

Cell Culture
CELLSTAR® Cell Culture Dishes

Cell Culture Dishes

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Improved cell adhesion through physical surface
treatment/

Maximal transparency for excellent microscopic
analysis/

Sterile and user-friendly packaging/

Easy stacking

nonpyrogenic

Surface treatment: TC, Vent nock: yes, Sterile: +Compartments

HeightØ nominal
size

Growtharea

SterileQty. inner /
outer

10 mm

35 mm

8.7 cm²

10 ml+

10 / 740

10 mm

35 mm

≥0.08 ml

9 ml+

10 / 740

628160

15 mm

60 mm

21 cm²6 ml - 7 ml

28 ml

+

10 / 600

628160-TRI

15 mm

60 mm

21 cm²6 ml - 7 ml

28 ml

+

10 / 300

66416020 mm

100 mm58 cm²16 ml - 17 ml

100 ml+15 / 360

664160-TRI

20 mm

100 mm58 cm²16 ml - 17 ml

100 ml+15 / 180

63916020 mm

145 mm143 cm²

25 ml - 27 ml

240 ml+5 / 120639160-TRI

20 mm

145 mm143 cm²

25 ml - 27 ml

240 ml+5 / 120

Item no.

6271606271704

Growtharea / unit

0.93 cm²

Working volume

Total
volume≥3 ml

Triplepacked

yes
yes
yes

/ For exact dimensions of our cell culture dishes, please refer to the product data sheets on our website.

Cell Culture Dishes - Advanced TC
The Advanced TC surface provides optimal
conditions for the cultivation of sensitive andfastidious cells or the usage of restricted
growth conditions.

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Consistent and even cell attachment/

Homogeneous and optimised cell growth

nonpyrogenic

Surface treatment: Advanced TC, Vent nock: yes, Sterile: +Item no.

HeightØ nominal size

Growth area

Working volume

Total volume

SterileQty. inner / outer

627960

10 mm

35 mm

8.7 cm²

≤3 ml

10 ml

+

10 / 740

628960

15 mm

60 mm

21 cm²6 ml - 7 ml

28 ml

+

10 / 600

www.gbo.com

/ 49

Cell Culture
CELLSTAR® Cell Culture Dishes

Item no.

HeightØ nominal size

Growth area

Working volume

Total volume

SterileQty. inner / outer

66496020 mm

100 mm58 cm²16 ml - 17 ml

100 ml+15 / 360

63996020 mm

145 mm143 cm²

25 ml - 27 ml

240 ml+5 / 120/ Forum No. 12: Advanced TC: An innovative surface improving cellular assays (F071104)/ Application Report „Advanced TC for improving the cultivation / differentiation of embryonic stem cells“ (F076036)

Cell Culture Dishes - CELLCOAT®
/

Improved adhesion and cell proliferation/

Reduced-serum or serum-free cultivation/

Improved growth of primary cells

Surface treatment: CELLCOAT®, Vent nock: yes
Item no.

50 /HeightØ nominal size

Growth area

Protein coating

Working volume

Total volume

Qty. inner / outer

628950

15 mm

60 mm

21 cm²

Collagen Type I

6 ml - 7 ml

28 ml

20 / 10066495020 mm

100 mm58 cm²

Collagen Type I

16 ml - 17 ml

100 ml10 / 40628940

15 mm

60 mm

21 cm²Poly-D-Lysine

6 ml - 7 ml

28 ml

20 / 10066494020 mm

100 mm58 cm²Poly-D-Lysine

16 ml - 17 ml

100 ml10 / 40628930

15 mm

60 mm

21 cm²Poly-L-Lysine

6 ml - 7 ml

28 ml

20 / 100

628920

15 mm

60 mm

21 cm²

Fibronectin

6 ml - 7 ml

28 ml

5 / 2066492020 mm

100 mm58 cm²

Fibronectin

16 ml - 17 ml

100 ml5 / 10628910

15 mm

60 mm

21 cm²Laminin

6 ml - 7 ml

28 ml

5 / 2066491020 mm

100 mm58 cm²Laminin

16 ml - 17 ml

100 ml10 / 10

www.gbo.com

Cell Culture
CELLSTAR® Cell Culture Dishes

Cell Culture Dishes
Cell-Repellent Surface
Cell culture vessels with cell-repellent surface
reliably prevent cell attachment in suspension
cultures of semi adherent / adherent celllines where standard hydrophobic surfaces
generally used for suspension culture areinsufficient.

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Raw material: PS, Surface treatment: cell-repellent, Vent nock: yes, Sterile: +Item no.

HeightØ nominal size

Working volume

Total volume

SterileQty. inner / outer

627979

10 mm

35 mm

≤3 ml

10 ml

+

10 / 40628979

15 mm

60 mm

6 ml - 7 ml

28 ml

+

10 / 2066497020 mm

100 mm

16 ml - 17 ml

100 ml

+1/5

CELLview Dish
The CELLview cell culture dish combines
the convenience of a standard size 35 mm
disposable plastic cell culture dish with theoptical quality of glass, providing superior
high-resolution microscopic images of in-vitro
cultivated cultures.
/

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

Embedded glass bottom for maximal planarity

nonpyrogenic

Ø: 35 mm, Bottom: glass, Vent nock: yes, Sterile: +Compartments

Growtharea

Surface treatment

Working
volumeTotal
volume627861

1

8.7 cm²

untreated

2.5 ml 5 ml

6278601

8.7 cm²

TC6279651

8.7 cm²

Advanced TC

627871

4

Item no.

Growtharea / unit

1.9 cm²

untreated

Total
volume(well)

SterileQty. inner /
outer

10 ml

+

10 / 40

2.5 ml 5 ml

10 ml

+

10 / 40

2.5 ml 5 ml

10 ml

+

10 / 40+

10 / 40

1.5 mlWorking
volume (well)

0.1 ml 0.5 ml

www.gbo.com

/ 51

Cell Culture
CELLSTAR® Cell Culture Dishes

Compartments

Item no.

Growtharea

Growtharea / unit

Surface treatment

Working
volumeTotal
volumeTotal
volume(well)

Working
volume (well)

SterileQty. inner /outer6278704

1.9 cm²

TC

1.5 ml0.1 ml 0.5 ml+

10 / 406279754

1.9 cm²

Advanced TC

1.5 ml0.1 ml 0.5 ml+

10 / 40/ Application Note „Protein localisation using confocal laser scanning microscopy“ (F073101)/ Application Note „Live cell imaging on Golgi morpholgy using the CELLview dish“ (F074048)

OneWell Plate
FourWell PlateSTERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

For the cultivation of a large number of cells the
OneWell Plate with ANSI standard dimensions
offers the best possible conditions.

/

The FourWell Plates allows users to culture cells
on slides while remaining in accordance with an
HTS-compatible plate. This four-chambered plateis particularly suitable for parallel experiments.

nonpyrogenic

Height: 14.4 mm, Length: 127.8 mm, Width: 85.5 mm, Lid: yes, Vent nock: yes, Sterile: +Item no.

Well format

96077307

467019016701801

Growth area

www.gbo.com

Product colour

Total volume (well)

SterileQty. inner / outersuspension

l clear18.6 ml+

8 / 3295 cm²suspension

l clear113.7 ml+

8 / 3295 cm²TCl clear113.7 ml+

8 / 32/ Sample packs are available on request.

52 /Surface treatment

Cell Culture
CELLSTAR® Cell Culture Multiwell Plates/

Watch our video:

CELLSTAR®
CELL CULTURE MULTIWELL PLATES/With hydrophilic
surface (TC surface
treatment) for improved cell adhesion/

With hydrophobic
surface for suspension
cultures and hybridoma
cells

/

High clarity and low
autofluorescence/

Alphanumeric wellcodingFor both adherent and suspension cell culture, choose
Greiner Bio-One’s multiwellplates in either 6, 12, 24 or 48
well format.
The special chimney wells in
conjunction with low evaporation lids significantly reduce therisk of cross-contamination between the wells. The plates areeasily stacked, both with andwithout lids. Due to their ad-vanced design, the plates still
exhibit important features likeconstant heat and gas exchangeeven when stacked. Uniform
plate dimensions (well depth, diameter and distance between
the wells) comply with ANSI
standards, enabling easy manual and automated handling. Each
plate is sterile, free of detectable DNase, RNase, human DNA,is non-pyrogenic and has a printed lot number for traceability.

www.gbo.com

/ 53

Cell Culture
CELLSTAR® Cell Culture Multiwell Plates

Multiwell Plates
6 / 12 / 24 / 48 Well Format

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

High transparency/

Alphanumeric well coding/

With hydrophilic surface (TC surface treatment) for
improved cell adhesion/

With hydrophobic surface for suspension culturesand hybridoma cells

nonpyrogenic

Bottom: solid, Raw material: PS, Lid: yes, condensation rings, Sterile: +Well format

Growth area / unit

Surfacetreatment

ProductcolourTotal volume(well)

Working volume(well)

SterileQty. inner /outer6571606

9.6 cm²TC

l clear

16 ml

2 ml - 5 ml+

1 / 100

66518012

3.9 cm²TC

l clear

6.5 ml2 ml - 4 ml+

1 / 100

66216024

1.9 cm²TC

l clear

3.3 ml

0,5 ml - 1,5 ml+

1 / 100

677180

48

1 cm²

TC

l clear

1.7 ml

0.5 ml - 1 ml+

1 / 100

6571856suspension

l clear

16 ml+

1 / 100

66510212

suspension

l clear

6.5 ml+1 / 100

66210224

suspension

l clear

3.3 ml+1 / 100

67710248

suspension

l clear

1.7 ml+1 / 100

Item no.

Multiwell Plates
Advanced TC
The Advanced TC surface provides optimal
conditions for the cultivation of sensitive andfastidious cells or the usage of restricted
growth conditions.
/

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

Consistent and even cell attachment

nonpyrogenic

Bottom: solid, Raw material: PS, Surface treatment: Advanced TC, Lid: yes, condensation rings, Sterile: +Item no.

54 /Well format

Growth area / unit

6579606

9.6 cm²

66598012

3.9 cm²

www.gbo.com

Product colour

Total volume (well)

Working volume (well)

SterileQty. inner / outerl clear

16 ml

2 ml - 5 ml+

1 / 100

l clear

6.5 ml2 ml - 4 ml+

1 / 100

Cell Culture
CELLSTAR® Cell Culture Multiwell Plates

Item no.

Well format

Growth area / unit

66296024

1.9 cm²

67798048

1 cm²

Product colour

Total volume (well)

Working volume (well)

SterileQty. inner / outerl clear

3.3 ml

0,5 ml - 1,5 ml+

1 / 100

l clear

1.7 ml

0.5 ml - 1 ml+

1 / 100/ Forum No. 12: Advanced TC: An innovative surface improving cellular assays (F071104)/ Application Report „Advanced TC for improving the cultivation / differentiation of embryonic stem cells“ (F076036)

Multiwell Plates
CELLCOAT®
/

Improved adhesion and cell proliferation/

Reduced-serum or serum-free cultivation/

Improved growth of primary cells

Bottom: solid, Raw material: PS, Surface treatment: CELLCOAT®, Lid: yes, condensation rings
Total volume (well)

Working volume(well)

Qty. inner /outerl clear16.1 ml

2 ml - 5 ml

5 / 50Collagen Type I

l clear

3.3 ml

0,5 ml - 1 ml

5 / 509.6 cm²

Poly-D-Lysine

l clear16.1 ml

2 ml - 5 ml

5 / 5024

1.9 cm²

Poly-D-Lysine

l clear

3.3 ml

0,5 ml - 1 ml

5 / 506579306

9.6 cm²

Poly-L-Lysine

l clear16.1 ml

2 ml - 5 ml

5 / 5066293024

1.9 cm²

Poly-L-Lysine

l clear

3.3 ml

0,5 ml - 1 ml

5 / 50Item no.

Well format

Growth area / unit

Protein coating

6579506

9.6 cm²

Collagen Type I

66295024

1.9 cm²

6579406662940

Product colour

www.gbo.com

/ 55

Cell Culture
CELLSTAR® Cell Culture Multiwell Plates

Multiwell Plates
Cell-Repellent Surface
Cell culture vessels with cell-repellent surface
reliably prevent cell attachment in suspension
cultures of semi adherent / adherent celllines where standard hydrophobic surfaces
generally used for suspension culture areinsufficient.

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Well profile: F-bottom, Bottom: solid, Raw material: PS, Surface treatment: cell-repellent, Lid: yes, condensation rings,
Sterile: +Item no.

Well format

Product colour

Total volume (well)

Working volume (well)

SterileQty. inner / outer

6579706

l clear16.1 ml

2 ml - 5 ml+

1/566297024

l clear

3.3 ml

0,5 ml - 1,5 ml+

1/567797048

l clear

1.7 ml

0.5 ml - 1 ml+

1/5

/ Forum No. 17: CELLSTAR® Cell Culture Vessels with Cell-Repellent Surface (F073777)/ Application Report “Advantage of CELLSTAR® Cell Culture Vessels with Cell-Repellent Surfaces for 3-D Cell Culture in Hydrogels” (F073792).

56 /

www.gbo.com

Cell Culture
ThinCert® Cell Culture Inserts for 6, 12 and 24 Well Multiwell Plates/

Forum No. 8: ThinCert ®
cell culture products –Overview (F073017)/

Application Note
„Immuno cytochemistry“(F073100)/

For further information
and application notes
please refer to our
website: www.gbo.com

THINCERT® CELL CULTURE INSERTS
FOR 6, 12 AND 24 WELL MULTIWELL PLATES
For advanced cell and tissue culture applications,
Greiner Bio-One offers a broad
range of membrane supports- ThinCert®.

/

Stable clear
polystyrene housing/

Sealed PET capillarypore membrane/

Pre-configured
multiwell plates with
ThinCert ® cell culture
inserts available on
requestCombining 6 different membrane specifications (pore sizeand density) in geometries tofit 6, 12 and 24 well plates, theThinCert® cell culture inserts
are suitable for a wide range ofapplications including transport,secretion and diffusion studies,migrational experiments, cytotoxicity testing, co-cultures,trans epithelial electric resist-ance (TEER) measurements, aswell as primary cell culture.
ThinCert ® cell culture inserts
are compatible with standard
CELLSTAR ® multiwell plates
from Greiner Bio-One, and are
pre-packed together with therequisite number of plates. The
automated production processincludes double optical controlof each insert produced, ensuring that any biological contamination is avoided. The sterility ofthe single blisterpacked inserts
and multiwell plates is ensured
by irradiation.

www.gbo.com

/ 57

Cell Culture
ThinCert® Cell Culture Inserts for 6, 12 and 24 Well Multiwell Plates

ThinCert® Cell Culture Inserts
6 WellSTERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Hanging geometry/

Improved cell adhesion through physical surface
treatment/

Simplified pipetting due to self-lift geometry/

Enhanced pipetting access and gas exchange

nonpyrogenic

Feature: 4 multiwell plates / box, Height: 16.25 mm, Ø internal: 24.85 mm, Ø external: 27.85 mm, Cultural surface: 452.4
mm², Surface treatment: TC, Working volume (ThinCert®): 1 ml - 3.6 ml, Working volume (well): 2 ml - 4.15 ml, Sterile: +Item no.

58 /Pore density

Ø poreOptical membrane properties

SterileQty. inner / outer

6576401 x 10⁸ / cm²

0.4 µmtranslucent+

1 / 246576412 x 10⁸/ cm²

0.4 µmclear+

1 / 246576102 x 10⁸/ cm²

1 µm

clear

+

1 / 246576300,6 x 10⁶ / cm²

3 µm

clear

+

1 / 246576312 x 10⁸/ cm²

3 µm

translucent+

1 / 24657638

0,15 x 10⁶ / cm²

8 µm

translucent+

1 / 24www.gbo.com

Cell Culture
ThinCert® Cell Culture Inserts for 6, 12 and 24 Well Multiwell Plates

ThinCert® Cell Culture Inserts
12 Well

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Hanging geometry/

Improved cell adhesion through physical surface
treatment/

Simplified pipetting due to self-lift geometry/

Enhanced pipetting access and gas exchange

nonpyrogenic

Feature: 4 multiwell plates / box, Height: 16.25 mm, Ø internal: 13.85 mm, Ø external: 15.85 mm, Cultural surface: 113.1
mm², Surface treatment: TC, Working volume (ThinCert®): 0.3 ml - 1 ml, Working volume (well): 1 ml - 2 ml, Sterile: +Item no.

Pore density

Ø poreOptical membrane properties

SterileQty. inner / outer

6656401 x 10⁸ / cm²

0.4 µmtranslucent+

1 / 486656412 x 10⁸/ cm²

0.4 µmclear+

1 / 486656102 x 10⁸/ cm²

1 µm

clear

+

1 / 486656300,6 x 10⁶ / cm²

3 µm

clear

+

1 / 486656312 x 10⁸/ cm²

3 µm

translucent+

1 / 48665638

0,15 x 10⁶ / cm²

8 µm

translucent+

1 / 48www.gbo.com

/ 59

Cell Culture
ThinCert® Cell Culture Inserts for 6, 12 and 24 Well Multiwell Plates

ThinCert® Cell Culture Inserts
24 Well

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Hanging geometry/

Improved cell adhesion through physical surface
treatment/

Simplified pipetting due to self-lift geometry/

Enhanced pipetting access and gas exchange

nonpyrogenic

Feature: 2 multiwell plates / box, Height: 16.25 mm, Ø internal: 8.4 mm, Ø external: 10.4 mm, Cultural surface: 33.6 mm²,
Surface treatment: TC, Working volume (ThinCert®): 0.1 ml - 0.35 ml, Working volume (well): 0.4 ml - 1.2 ml, Sterile: +Item no.

Pore density

Ø poreOptical membrane properties

SterileQty. inner / outer6626401 x 10⁸ / cm²

0.4 µmtranslucent+

1 / 486626412 x 10⁸/ cm²

0.4 µmclear+

1 / 486626102 x 10⁸/ cm²

1 µm

clear

+

1 / 486626300,6 x 10⁶ / cm²

3 µm

clear

+

1 / 486626312 x 10⁸/ cm²

3 µm

translucent+

1 / 48662638

0,15 x 10⁶ / cm²

8 µm

translucent+

1 / 48

ThinCert® Plate
6 / 12 Well

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Optimised for use with ThinCert® cell culture
inserts/

Deep wells for an increased volume of medium in
air-lift culture/

Notches for fixed insert position/

Available in 6 and 12 well format

nonpyrogenic

Height: 39.5 mm, Length: 129.5 mm, Width: 86.6 mm, Lid: yes, condensation rings, Sterile: +Item no.

65711060 /www.gbo.com

Well format

Working volume (well)

SterileQty. inner / outer6≤20 ml

+1 / 50Cell Culture
ThinCert® Cell Culture Inserts for 6, 12 and 24 Well Multiwell Plates

Item no.

665110Well format

Working volume (well)

SterileQty. inner / outer

12

≤4 ml

+

1 / 60www.gbo.com

/ 61

Cell Culture
ThinCert® 96 Well HTS Insert For high-throughput applications/

Application Note
“High-throughput
in-vitro airway modelling with ThinCert®
96 Well HTS Insert”(F073140)/

96 well system forhigh-throughput
applications/

Automation-friendly
geometry/

Pore sizes of 0.4 µm,
3 µm and 8 µm covering
a range of different
applications/

High permeability andtransparency for
optimal diffusion rates
and optical features

THINCERT® 96 WELL HTS INSERTFOR HIGH-THROUGHPUT APPLICATIONSThinCert® 96 well HTS insert isthe latest high-throughput addition to our ThinCert® family which opens doors to automation and offers an efficientsolution to scale up your membrane-based cell culture application. Take advantage of theassay miniaturisation to maximise both time and cost savings when compared to single-insert solutions.
ThinCert ® 96 well HTS inserts offer a diverse selection of membrane specifications (pore sizeand density) within a 96 well format. The automation-friendly inserts are highly versatile and canbe utilised for various applications such as transport, secre-

62 /www.gbo.com

tion, and diffusion studies, migration/invasion experiments,
co-cultures, cytotoxicity tests,
TEER measurements (Trans Epithelial Electric Resistance) andthe generation of tissue models(e.g.,endothelia and epithelia).
The two-component systemcomprises a 96 well porous
membrane plate manufactured
entirely from polycarbonate (PC)and a corresponding polystyrene(PS) receiver plate. The tissue
culture treated PC membraneallows for an ideal exchange ofnutrients and substances, creating in vivo-like cultivation conditions to facilitate optimal cell
growth, monolayer formation,and tissue differentiation.

Cell Culture
ThinCert® 96 Well HTS Insert For high-throughput applications

ThinCert ® 96 Well HTS Insert(Membrane plates and receiver plates)

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Pore sizes of 0.4 µm, 3 µm and 8 µm covering a
range of different applications/

High permeability and transparency for optimal
diffusion rates and optical features/

Working volume per well (membrane plate): 15 - 160µl/

Working volume per well (receiver plate): 120 - 300µl

nonpyrogenic

NEW

Well format: 96, Surface treatment: TC, Lid: yes, condensation rings, Sterile: +Item no.

Pore density

Ø poreOptical membrane properties

SterileQty. inner / outer

6556401 x 10⁸ / cm²

0.4 µmtranslucent+

1/5

6556412 x 10⁷ / cm²

0.4 µmoptimised transparency+

1/56556302 x 10⁶ / cm²

3 µm

clear

+

1/5

6556801 x 10⁵ / cm²

8 µm

clear

+

1/5

Receiver plates for
ThinCert ® 96 Well HTS Insert

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Reduced wicking effect/High permeability and transparency for optimal
diffusion rates and optical features/

Working volume per well (receiver plate): 120 - 300µl

nonpyrogenicNEW

Lid: yes, condensation rings, Sterile: +Item no.

Growth area

65516965516753 mm²

Surface treatment

SterileQty. inner / outeruntreated+

8 / 32

TC+

8 / 32www.gbo.com

/ 63

Cell Culture
CELLSTAR® Cell Culture Microplates/

Application Note
„Establishing a cell
culture assay based on
TR-FRET for screening
G-Protein-coupled
receptors“ (F074058)/

Application Note „Selection of cell culture
surfaces for the adipogenic differentiation of
hMSCs“ (F010003)/

Available with different
cell culture surfaces
for optimal cell culture
conditions/

Footprint compatible with automatedsystems/

Alphanumeric wellcodingCELLSTAR®
CELL CULTURE MICROPLATESCell culture treated microplates are available in thefollowing versions: 96, 384,
1536 well format.
Depending on the application,
the well profile is a key feature in a 96 well cell culture
microplate. The chimney well
cell culture microplate has thesame well profile as the standard F-bottom plate. The difference to the standard plate is
the chimney-like arrangementof the wells i.e. each well stands
on its own. Therefore the riskof contamination from sample
material being carried over isminimised. Clear-bottom mi-

64 /www.gbo.com

croplates have pigmented walls
and a transparent thin film bottom, the so-called µClear ® bottom. In contrast to our standard
microplates with a solid polystyrene bottom, they are ideal
for cell culture and microscopic applications using fluorescence or luminescence detection methods.
For many applications, a reduction of the sample volume is an
important feature. 96 well halfarea microplates offer an interesting alternative here. They canbe pipetted automatically as wellas manually without any problem
and allow a reduction of the sample volume up to 50 %.

Cell Culture
CELLSTAR® Cell Culture Microplates

Cell Culture Microplates
96 Well

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

With U-bottom, V-bottom or F-bottom/

Clear / black / white/

Chimney well design, raised wells andcondensation rings in lids preventcross-contamination/

Improved cell adhesion through physical surface
treatment

nonpyrogenic

Well format: 96, Bottom: solid, Raw material: PS, Surface treatment: TC, Sterile: +Growth area
/ unitWell profile

Product colour

Working volume(well)

Lid

SterileQty. inner /outer65016035 mm²

U-bottoml clear

40 µl - 280 µl

no+1 / 100

65018035 mm²

U-bottoml clear

40 µl - 280 µl

yes+

1 / 100

65116028 mm²

V-bottoml clear

40 µl - 200 µl

no+1 / 100

65118028 mm²

V-bottoml clear

40 µl - 200 µl

yes+

1 / 100

65516034 mm²

F-bottom / Chimney Welll clear

25 µl - 340 µl

no+1 / 100

65516234 mm²

F-bottom / Chimney Welll clear

25 µl - 340 µl

no+5 / 100

65518034 mm²

F-bottom / Chimney Welll clear

25 µl - 340 µl

yes, condensation rings+

1 / 100

65518234 mm²

F-bottom / Chimney Welll clear

25 µl - 340 µl

yes, condensation rings+

10 / 160

65507334 mm²

F-bottom / Chimney Welll white

25 µl - 340 µl

no+10 / 4065508334 mm²

F-bottom / Chimney Welll white

25 µl - 340 µl

yes, condensation rings+

8 / 3265507934 mm²

F-bottom / Chimney Welll black

25 µl - 340 µl

no+10 / 4065508634 mm²

F-bottom / Chimney Welll black

25 µl - 340 µl

yes, condensation rings+

8 / 32Item no.

/ For selected products Greiner Bio-One also offers user-friendly bulk packaging.
/ Barcode labelling on request

www.gbo.com

/ 65

Cell Culture
CELLSTAR® Cell Culture Microplates

Cell Culture Microplates
96 Well - µClear®
Clear-bottom microplates have pigmented
walls and a transparent thin film bottom, theso-called μClear ® bottom. In contrast to ourstandard microplates with a solid polystyrene
bottom, they are ideal for cell culture andmicroscopic applications using fluorescenceor luminescence detection methods.
STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Well format: 96, Growth area / unit: 34 mm², Well profile: F-bottom / Chimney Well, Bottom: µClear®, Raw material: PS,
Surface treatment: TC, Working volume (well): 25 µl - 340 µl, Sterile: +Item no.

Product colour

Lid

SterileQty. inner / outer

655088l whiteno+10 / 40655098l whiteyes, condensation rings+

8 / 32655087l blackno+10 / 40655090l black

yes, condensation rings+

8 / 32Cell Culture Microplates
96 Well - Half Area
For many applications, a reduction of thesample volume is an important feature. 96
well half area microplates offer an interestingalternative here. They can be pipetted
automatically as well as manually without any
problem and allow a reduction of the sample
volume up to 50 %.
STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Well format: 96, Growth area / unit: 15 mm², Well profile: F-bottom, Raw material: PS, Surface treatment: TC, Plate
design: half area, Working volume (well): 15 µl - 175 µl, Lid: yes, Sterile: +Item no.

66 /BottomProduct colour

SterileQty. inner / outer

675180solid

l clear+8 / 32675083solid

l white+8 / 32675086solid

l black+

8 / 32www.gbo.com

Cell Culture
CELLSTAR® Cell Culture Microplates

Item no.

675090BottomµClear®

Product colourl black

SterileQty. inner / outer+

8 / 32/ For selected products Greiner Bio-One also offers user-friendly bulk packaging.

Cell Culture Microplates
96 Well - CELLCOAT®
/

Improved adhesion and cell proliferation/

Reduced-serum or serum-free cultivation/

Improved growth of primary cells

Well format: 96, Growth area / unit: 34 mm², Well profile: F-bottom / Chimney Well, Raw material: PS, Surface
treatment: CELLCOAT®, Working volume (well): 25 µl - 340 µl, Lid: yes, condensation rings
Item no.

BottomProtein coating

Product colour

Qty. inner / outer

655950solid

Collagen Type I

l clear5 / 20655956µClear®

Collagen Type I

l black

5 / 20655940solid

Poly-D-Lysine

l clear5 / 20655944µClear®

Poly-D-Lysine

l white5 / 20655946µClear®

Poly-D-Lysine

l black

5 / 20655948µClear®

Poly-D-Lysine

l black

20 / 120655930solid

Poly-L-Lysine

l clear5 / 20655936µClear®

Poly-L-Lysine

l black

5 / 20www.gbo.com

/ 67

Cell Culture
CELLSTAR® Cell Culture Microplates

Cell Culture Microplates
96 Well - Advanced TC
The Advanced TC surface provides optimal
conditions for the cultivation of sensitive andfastidious cells or the usage of restricted
growth conditions.
/

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

Consistent and even cell attachment

nonpyrogenic

Well format: 96, Growth area / unit: 34 mm², Well profile: F-bottom / Chimney Well, Raw material: PS, Surface
treatment: Advanced TC, Working volume (well): 25 µl - 340 µl, Lid: yes, condensation rings, Sterile: +Item no.

BottomProduct colour

SterileQty. inner / outer655980solid

l clear+1 / 100

655983µClear®

l white+8 / 32655986µClear®

l black+

8 / 32

Suspension Culture Microplates
96 Well

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Hydrophobic surface, ideal for suspension cultures, hybridoma and embryonic stem cells/

Solid bottom

nonpyrogenic

Well format: 96, Bottom: solid, Raw material: PS, Surface treatment: suspension, Sterile: +Item no.

68 /Well profile

Product colour

650185

U-bottom

655185F-bottom / Chimney Well

www.gbo.com

Working volume (well)

Lid

SterileQty. inner / outerl clear

40 µl - 280 µl

yes+

60 / 240l clear

25 µl - 340 µl

yes, condensation rings+

60 / 240

Cell Culture
CELLSTAR® Cell Culture Microplates

Cell Culture Microplates
384 Well/Clear / black / white/

Solid bottom or µClear ® film bottom/

Barcode labelling on request/

Alphanumeric well coding

Well format: 384, Growth area / unit: 10 mm², Well profile: F-bottom, Raw material: PS, Working volume (well): 15 µl - 110µl

Lid

SterileQty. inner /outerl clearno+10 / 40TCl clearyes+

8 / 32solidTC

l whiteno+10 / 40781080solidTC

l whiteyes+

8 / 32781079solid

TC

l blackno+10 / 40781086solidTC

l blackyes+

8 / 32781093µClear®

TC

l whiteno+10 / 40781098µClear®

TC

l whiteyes+

8 / 32781092µClear®

TC

l blackno+10 / 40781091µClear®

TC

l blackyes+

8 / 32781090µClear®

TC

l black

yes+

20 / 120

781950solid

CELLCOAT®

Collagen Type I

l clear131 µlyes

5 / 20781956µClear®

CELLCOAT®

Collagen Type I

l black

131 µlyes

5 / 20781940solid

CELLCOAT®

Poly-D-Lysine

l clear131 µlyes

5 / 20781944µClear®

CELLCOAT®

Poly-D-Lysine

l white

131 µlyes

5 / 20781946µClear®

CELLCOAT®

Poly-D-Lysine

l black

131 µlyes

5 / 20781948µClear®

CELLCOAT®

Poly-D-Lysine

l black

131 µlyes

20 / 120

781930solid

CELLCOAT®

Poly-L-Lysine

l clear131 µlyes

5 / 20781936µClear®

CELLCOAT®

Poly-L-Lysine

l black

131 µlyes

5 / 20781983µClear®

Advanced TCl white

131 µlyes+

8 / 32781986µClear®

Advanced TC

l black

131 µlyes+

8 / 32Item no.

BottomSurface treatment

781165solidTC781182solid

781073

Protein coating

Product colour

Total volume (well)www.gbo.com

/ 69

Cell Culture
CELLSTAR® Cell Culture Microplates

Cell Culture Microplates
384 Well - Small Volume
HiBase/Perfect for top reading even at low working
volumes/

Savings in reagent similar to 1536 well microplates/

Made of black / white polystyrene for fluorescenceor luminescence measurements

Well format: 384, Growth area / unit: 2.7 mm², Well profile: F-bottom, Bottom: solid, Raw material: PS, Plate geometry:
HiBase, Plate design: Small Volume, Working volume (well): 4 µl - 25 µl, Lid: yes

Item no.

Surface treatment

Protein coating

Product colour

SterileQty. inner / outer

784080TCl white+8 / 32784086TC

l black+

8 / 32784946

CELLCOAT®

Poly-D-Lysine

l black

5 / 30Cell Culture Microplates
1536 Well/

Clear / black / white/

Solid bottom or µClear ® film bottom/

Barcode labelling on request

Well format: 1536, Growth area / unit: 2.3 mm², Well profile: F-bottom, Raw material: PS, Plate geometry: HiBase,
Working volume (well): 3 µl - 10 µl

Item no.

70 /BottomSurface treatment

782180solid

782073

Product colour

Lid

SterileQty. inner / outerTCl clearyes+

1 / 32solidTC

l whiteno+15 / 60

782080solidTC

l whiteyes+

10 / 40782078solidTC

l black

no+15 / 60

782086solidTC

l blackyes+

10 / 40782093µClear®

TC

l whiteno+15 / 60

782092µClear®

TC

l black

no+15 / 60782946µClear®

CELLCOAT®

l blackyes

www.gbo.com

Protein coating

Poly-D-Lysine

5 / 20Cell Culture
CELLSTAR® Cell Culture Microplates

Lids
All sterile lids are non-cytotoxic.

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

nonpyrogenic

Description: Lid, Raw material: PS
Item no.

HeightProduct colour

Condensation rings

Lid type

6561019 mm

l clearnohigh6561619 mm

l clearnohigh6561709 mm

l clearyeshigh6561719 mm

l clearyeshigh6561906 mm

l clearnoflat656191

6 mm

l clearnoflat691101

4.8 mml clearnoultra low

691161

4.8 mml clearnoultra low

SterileQty. inner / outer1 / 100+

1 / 100
1 / 100+

1 / 100
20 / 200+

20 / 200
25 / 100+

25 / 100

www.gbo.com

/ 71

Cell Culture
CELLSTAR® Cell Culture Tubes/

Application Note:
Cultivation of Suspension and Hybridoma
Cells in CELLSTAR®
CELLreactor Tubes(F073918)/

Application Note:
Superior protein yields
in suspension CHO cells
using FectoPRO™-mediated transient transfection in CELLSTAR®
CELLreactor (F073926)/

Bioreactor forsuspension andspheroid cells/

Expansion of aerobic
bacteria, yeast andmicroorganisms/

Storage of components
and liquids requiring
gas exchange

CELLSTAR® CELL CULTURE TUBESCELLSTAR® CELLreactor tube
can be used as small bioreactor for suspension and spheroid
cell culture, facilitating miniaturisation of large-scale setups and maximising the number of parallel experiments.
Each CELLreactor tube cap features several holes and a membrane with a pore size of 0.2 μm
to guarantee maximal sterility
while providing excellent gas exchange. In case the aeration has
to be reduced, individual openings can be sealed.
Agitation of internal liquids isachieved with standard shaking
lab equipment minimising foam
formation and shearing forces

72 /www.gbo.com

induced by integrated mixing
devices. Compared to cell culture and spinner flask as well asother cultivation disposables, notransfer for cell harvest is required. Based on the conicaldesign, the tubes fit in standard 15 ml / 50 ml centrifuge rotors and cells can be spun downin the same tube. In addition tocell culture applications, the
CELLSTAR® CELLreactor tube
can also be applied for the expansion of aerobic bacteria,
yeast or other microorganisms
in shaken cultures as well as
storage of components and liquids requiring gas exchange.

Cell Culture
CELLSTAR® Cell Culture Tubes

CELLreactor
15 ml and 50 ml polypropylene tube with filter
screw cap

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

For cultivation of suspension cells and expansionof aerobic microorganisms/

Facilitates a high number of parallel experiments/

Maximal sterility and excellent gas exchange/

Conical tube design and in-tube harvest

nonpyrogenic

Graduation: yes, Writing field: yes, Raw material: PP, Bottom shape: conical, Cap design: filter screw cap, Sterile: +Item no.

HeightØ

Cap colour

188241

120 mm

17 mm

227245115 mm

30 mm

Working volume

Nominal volume

SterileQty. inner / outerl blue1 ml - 5 ml

15 ml

+

20 / 500l blue1 ml - 35 ml

50 ml

+

20 / 500/ Application Note: Cultivation of Suspension and Hybridoma Cells in CELLSTAR® CELLreactor Tubes (F073918)/ Application Note: Superior protein yields in suspension CHO cells using FectoPRO™-mediated transient transfection in CELLSTAR®
CELLreactor (F073926)

CELLSTAR®
Cell Culture Tubes

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Manufactured from crystal clear polystyrene/

Improved cell adhesion through physical surface
treatment/

Available with screw cap, bayonet cap or two-position cap

nonpyrogenic

Raw material: PS, Surface treatment: TC, Sterile: +Working volume

Nominalvolumeno≤4 ml

12.4 mm

no100 mm

17 mm

no164160100 mm16.8 mmyes

191160

95 mm

18 mm

no

Item no.

HeightØSupport skirt

12016075 mm

12.4 mm12019075 mm

163160

SterileQty. inner /outer

4.5 ml+1 / 1.000≤4 ml

4.5 ml+25 / 2.000

l red≤12 ml

12 ml

screw cap+

5 / 1.000

l red≤12 ml

12 mlbayonet cap+

5 / 1.000≤12.5 ml

14 ml+

1 / 750

Cap colour

Cap designwww.gbo.com

/ 73

Cell Culture
EASYstrainer Cell Strainers/

Watch our video:

EASYSTRAINER CELL STRAINERS
With EASYstrainer cell strainers, Greiner Bio-One offers an
innovative and user-friendly
solution for the filtration of cellsuspensions.

74 /

/

Flexible fit, suitable
for tubes from 1.5 ml to
50 ml

/

Available Mesh Sizes:
20, 40, 70 und 100 µm/

No liquid overspill

www.gbo.com

EASYstrainer can be used, forexample, after enzymatic tissue
digestion for primary cell isolation or for cell preparation prior
to flow cytometry. The large cellstrainers fit on all conical 50 ml
tubes and are available withmesh sizes of 40, 70 and 100 μm.
EASYstrainer Small fits 15 ml
tubes as well as smaller tubes
and reaction vessels. It is available with mesh sizes of 20, 40,
70 and 100 μm.

The stackability of EASYstrainers
with different mesh sizes allows for the separation of cellswith different sizes in one step.
In addition, the upper part of
EASYstrainer Small can be inverted. This allows retained cells
to be rinsed out for further use.
EASYstrainer makes working
aseptically much easier: It canbe held via a surrounding rim ora handle in order to avoid accidental contact with the sterile
filter material. Additional safety is provided by the transparent blister packaging from which
the strainers can be conveniently and aseptically removed.

Cell Culture
EASYstrainer Cell Strainers

EASYstrainer

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Flexible fit, suitable for tubes from 1.5 ml to 50 ml/

Handle and ridged skirt for improved aseptic
handling/

Venting slot for fast filtration/

No liquid overspill/

Single packaging reduces risk of contamination

nonpyrogenic

recommended

Sterile: +Item no.

Description

Mesh size

Product colour

SterileQty. inner / outer

542040EASYstrainer for 50 ml tubes

40 µm

l green+1 / 5070 µm

l blue542070EASYstrainer for 50 ml tubes+

1 / 50EASYstrainer for 50 ml tubes

100 µml yellow

542000+1 / 50542120EASYstrainer Small for tubes:
1.5 / 5 / 15 ml and 12x75 mm

20 µm

l red

+

1 / 50542140EASYstrainer Small for tubes:
1.5 / 5 / 15 ml and 12x75 mm

40 µm

l green+1 / 50542170EASYstrainer Small for tubes:
1.5 / 5 / 15 ml and 12x75 mm

70 µm

l blue+1 / 50542100EASYstrainer Small for tubes:
1.5 / 5 / 15 ml and 12x75 mm

100 µml yellow+

1 / 50www.gbo.com

/ 75

The cultivation of cells as mass cultures has become increasingly
important over the past few decades. Mass cell cultures are mainly
used for the production of vaccines or recombinant proteins fortherapeutic approaches.

76 /www.gbo.com

Mass Cell Culture﻿

MASS CELL CULTURE/ CELLMASTER Cell Culture Roller Bottles
������������������������������������������������������������ 78
Roller Bottles Polystyrene�������������������������������79
Roller Bottles Polyethylene
Terephthalate (PET)�������������������������������������80/ CELLdisc Cell Culture Device���������������� 81
CELLdisc 1 / 4 / 8 / 12 / 16 / 24 / 40 Layers���������82
CELLdisc External Filter����������������������������������83
CELLdisc Closed Filling Caps���������������������������84
CELLhandle ����������������������������������������������������85
CELLstage Filling Accessory���������������������������85
CELLevator �����������������������������������������������������86
CELLring ��������������������������������������������������������86www.gbo.com

/ 77

Mass Cell Culture
CELLMASTER Cell Culture Roller Bottles/

For further information, please refer to our
website: www.gbo.com/

PS or PET roller bottles/

Different sizes with orwithout radially ribbed
surface/

Graduations from
200 to 2000 ml/

Seamless production
technique rules out
leaking seams

CELLMASTER
CELL CULTURE ROLLER BOTTLES
Roller bottles made of polystyrene or PET range in size from850 cm² to 4250 cm² growtharea and are available eitherwith standard or filter screw
caps. The standard screw cap
enables a tight closing and contamination-free cultivation. The
filter screw cap contains a membrane with a pore size of 0.2 μm
guarantees optimal protection
against contamination. It provides a high gas exchange rate
which enables a mass cultivationof cells using e.g. bicarbonate

78 /www.gbo.com

buffer and CO2 .
The product range includes ashort and a long form, labelled
as X and XL, accordingly. Bothsizes are available with a smoothor radially ribbed surface. The
ribbed design increases thegrowth area of the roller bottle
without changing space requirements.All roller bottles are sterilised by irradiation according tovalidated procedures (ISO 11137).
Endotoxin testing is conductedin accordance with USP 85 witha tolerance level of 0.03 EU/ml.

Mass Cell Culture
CELLMASTER Cell Culture Roller Bottles

Roller Bottles
Polystyrene

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

For adherent cell culture/

Manufactured from crystal clear polystyrene/

Certified USP Class VI end product testing/Lot number and expiry date on each bottle/

Filter screw cap with 0.2 µm pore size

nonpyrogenic

Ø: 122 mm, Graduation: yes, Raw material: PS, Surface treatment: TC, Sterile: +Flask design

HeightGrowth area

Cap colour

Surface

Total volume

Cap design

SterileQty. inner /outer6806601X

271 mm850 cm²

l bluesmooth2,520 mlscrew cap+

2 / 24

680665

1X

271 mm850 cm²

l bluesmooth2,520 mlscrew cap+

24 / 246806481X

271 mm850 cm²

l bluesmooth2,520 mlscrew cap+

24 / 486806581X

271 mm850 cm²

l bluesmooth2,520 mlfilter screw cap+

2 / 24

6806681X

271 mm850 cm²

l bluesmooth2,520 mlfilter screw cap+

24 / 24680645

1X

271 mm850 cm²

l bluesmooth2,520 mlfilter screw cap+

24 / 486816702.5 X

271 mm

2,125 cm²

l blueribbed2,300 mlscrew cap+

2 / 246816752.5 X

271 mm

2,125 cm²

l blueribbed2,300 mlscrew cap+

24 / 246816722.5 X

271 mm

2,125 cm²

l blueribbed2,300 mlfilter screw cap+

2 / 246826601 XL

500 mm 1,700 cm²

l bluesmooth4,970 mlscrew cap+

1 / 12682612

1 XL

500 mm 1,700 cm²

l bluesmooth4,970 mlscrew cap+

12 / 12682624

1 XL

500 mm 1,700 cm²

l bluesmooth4,970 mlscrew cap+

12 / 24682615

1 XL

500 mm 1,700 cm²

l bluesmooth4,970 mlfilter screw cap+

12 / 12682625

1 XL

500 mm 1,700 cm²

l bluesmooth4,970 mlfilter screw cap+

12 / 246826705 XL

500 mm 4,250 cm²

l blueribbed4,640 mlscrew cap+

1 / 126826725 XL

500 mm 4,250 cm²

l blueribbed4,640 mlscrew cap+

12 / 246826785 XL

500 mm 4,250 cm²

l blueribbed4,640 mlfilter screw cap+

1 / 12Item no.

www.gbo.com

/ 79

Mass Cell Culture
CELLMASTER Cell Culture Roller Bottles

Roller Bottles
Polyethylene Terephthalate (PET)STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

For adherent cell culture/

Certified USP Class VI end product testing/With standard screw cap

nonpyrogenic

Flask design: 1 X, Height: 271 mm, Ø: 116 mm, Growth area: 850 cm², Graduation: yes, Raw material: PET,
Surface: smooth, Total volume: 2,300 ml, Cap design: screw cap, Sterile: +Item no.

80 /Cap colour

SterileQty. inner / outer

680190l blue+1 / 18680195l blue+30 / 30

www.gbo.com

Mass Cell Culture
CELLdisc Cell Culture Device/

Watch our video:

CELLDISCCELL CULTURE DEVICE/

Mass Cell Culture/

Antibody, virus andvaccine production/

Production ofrecombinant ortherapeutic proteins

CELLdisc is a multilayer cell culture device covering a range of
250 cm² to 10,000 cm² growtharea. The innovative ergonomic design provides a versatilesystem from research scale toindustrial batches with a 40 %higher surface/volume ratio
then conventional multilayer
systems.
A centrally located ventilation
channel assures uniform distribution of gas throughout thedevice whereas the wide opening port simplifies manual filling.
The compact and robust cylindrical device is ideally suited forautomation and upscaling of cell

cultures. For the connection of
the individual layers a proprietary, particle, and adhesive freeassembly technique is used andthe complete end product is USPClass VI certified.
To guarantee an ideal cell culture environment CELLdisc isprovided with two surface treatments: Beside the TC surface
for standard cells and applications CELLdisc is also available
with the Advanced TC surface,
improving cultivation of sensitive cells, even under restricted
growth conditions and increasing cellular adhesion, proliferation and transfection rates.

www.gbo.com

/ 81

Mass Cell Culture
CELLdisc Cell Culture Device

CELLdisc
1 / 4 / 8 / 12 / 16 / 24 / 40 Layers

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

40 % higher surface/volume ratio than comparable
systems/

Easy operation and minimal space occupation/

Media exchange without contact to cell layers

nonpyrogenic

Ø: 200 mm, Raw material: PS, Cap design: screw cap, Sterile: +Item no.

82 /LayersHeightGrowth area

Surface treatment

Cap colour

Working volume

SterileQty. inner / outer

678101

1

61 mm

250 cm²TC

l red

15 ml - 50 ml+

1/8

6781044

93 mm

1,000 cm²

TC

l red

60 ml - 200 ml+

1/4

6781088135 mm

2,000 cm²

TC

l red

120 ml - 400 ml+

1/3

678112

12

177 mm3,000 cm²

TC

l red

180 ml - 600 ml+

1/2

67811616

220 mm4,000 cm²

TC

l red

240 ml - 800 ml+

1/2

67812424

304 mm6,000 cm²

TC

l red

360 ml - 1,200 ml+

1/2

67814040474 mm

10,000 cm²

TC

l red

600 ml - 2,000 ml+

1/1

6789044

93 mm

1,000 cm²

Advanced TC

l blue60 ml - 200 ml+

1/4

6789088135 mm

2,000 cm²

Advanced TC

l blue120 ml - 400 ml+

1/3

678912

12

177 mm3,000 cm²

Advanced TC

l blue180 ml - 600 ml+

1/2

67891616

220 mm4,000 cm²

Advanced TC

l blue240 ml - 800 ml+

1/2

67892424

304 mm6,000 cm²

Advanced TC

l blue360 ml - 1,200 ml+

1/2

67894040474 mm

10,000 cm²

Advanced TC

l blue600 ml - 2,000 ml+

1/1

www.gbo.com

Mass Cell Culture
CELLdisc Cell Culture Device

CELLdisc
External Filter

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Triple-packed CELLdiscs, pre-assembled withexternal filters/

USP VI certified venting filters, manufactured withreinforced hydrophobic PTFE membranes/

A defined pore size of 0.2 μm prevents
contamination and ensures sufficient airflow
to the cells

nonpyrogenic

Feature: External Filter, Ø: 200 mm, Raw material: PS, Surface treatment: TC, Cap design: screw cap, Sterile: +Item no.

LayersHeightGrowth area

Cap colour

Working volume

SterileQty. inner / outer

678101-EXF

1

61 mm

250 cm²

l red

15 ml - 50 ml+

1/4

678104EXF

4

93 mm

1,000 cm²

l red

60 ml - 200 ml+

1/3

678108EXF

8135 mm

2,000 cm²

l red

120 ml - 400 ml+

1/2

678112-EXF

12

177 mm3,000 cm²

l red

180 ml - 600 ml+

1/2

678116-EXF

16

220 mm4,000 cm²

l red

240 ml - 800 ml+

1/2

678124-EXF

24

304 mm6,000 cm²

l red

360 ml - 1,200 ml+

1/2

678140EXF

40

474 mm

10,000 cm²

l red

600 ml - 2,000 ml+

1/1

www.gbo.com

/ 83

Mass Cell Culture
CELLdisc Cell Culture Device

CELLdisc
Closed Filling Caps

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Triple-packed CELLdiscs, pre-assembled withexternal filters and closed filling cap/

Choice of single tubing (CF1) or double tubing (CF2)depending on desired emptying process/

Tubing with MPC type connector for quick and safe
connection /disconnection

nonpyrogenic

Ø: 200 mm, Raw material: PS, Surface treatment: TC, Cap design: screw cap, Sterile: +84 /Item no.

FeatureLayersHeightGrowth area

Cap colour

Working volume

SterileQty. inner /outer678101-CF1

hose connector without
Dip-In1

61 mm

250 cm²

l red

15 ml - 50 ml+

1/4

678104-CF1

hose connector without
Dip-In4

93 mm

1,000 cm²

l red

60 ml - 200 ml+

1/3

678104-CF2

hose connector with
Dip-In4

93 mm

1,000 cm²

l red

60 ml - 200 ml+

1/3

678108-CF1

hose connector without
Dip-In8

135 mm

2,000 cm²

l red

120 ml - 400 ml+

1/2

678108-CF2

hose connector with
Dip-In8

135 mm

2,000 cm²

l red

120 ml - 400 ml+

1/2

678112-CF1

hose connector without
Dip-In12

177 mm3,000 cm²

l red

180 ml - 600 ml+

1/2

678112-CF2

hose connector with
Dip-In12

177 mm3,000 cm²

l red

180 ml - 600 ml+

1/2

678116-CF1

hose connector without
Dip-In16

220 mm4,000 cm²

l red

240 ml - 800 ml+

1/2

678116-CF2

hose connector with
Dip-In16

220 mm4,000 cm²

l red

240 ml - 800 ml+

1/2

678124-CF1

hose connector without
Dip-In24

304 mm6,000 cm²

l red

360 ml 1,200 ml+

1/2

678124-CF2

hose connector with
Dip-In24

304 mm6,000 cm²

l red

360 ml 1,200 ml+

1/2

678140-CF1

hose connector without
Dip-In40

474 mm

10,000 cm²

l red

600 ml 2,000 ml+

1/1

678140-CF2

hose connector with
Dip-In40

474 mm

10,000 cm²

l red

600 ml 2,000 ml+

1/1

www.gbo.com

Mass Cell Culture
CELLdisc Cell Culture Device

CELLhandle

Item no.

/Gripping device for easy lifting and emptying oflarge-sized CELLdisc/

Enables single-hand usage

Qty. inner / outer

878074

1/1

CELLstage
Filling Accessory

Item no.

/Available for CELLdisc 4-24 layers andCELLdisc 40 layers/

Creates the optimum angle and position for
CELLdisc filling/

Stainless steel allows multiple sterilization
methods/

Suitable for left- and right handed users

Description

Qty. inner / outer

878072for CD4 - CD24

1/1878073for CD40

1/1

www.gbo.com

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Mass Cell Culture
CELLdisc Cell Culture Device

CELLevator
Easy and secured CELLdisc stacking

Item no.

/Maximum loading capacity 8 kg/

Space saving storage/

Autoclavable (120 °C, 2 bar)

Qty. inner / outer

878071

1/9

CELLring
Levelling ring/

Item no.

87807586 /www.gbo.com

Ensures exact planar positioning of CELLdisc

Qty. inner / outer

1/3

Mass Cell Culture
CELLdisc Cell Culture Device

www.gbo.com

/ 87

In 3D cell culture, cells form in a spatial orientation - similar tothe body. This enables, for example, the replacement of animal
experiments and - usually as high-throughput screening - researchon drugs, stem cells and tumor development.

88 /www.gbo.com

3D Cell Culture﻿

3D CELL CULTURE/ CELLSTAR ® Cell Culture Vessels CellRepellent Surface�������������������������������� 90
Cell Culture Dishes Cell-Repellent Surface������ 91
Cell Culture Flasks Cell-Repellent Surface������ 91
Multiwell Plates / Microplates Cell-Repellent
Surface��������������������������������������������������������92
SCREENSTAR Microplates Cell-Repellent
Surface��������������������������������������������������������93/ Magnetic 3D Cell Culture ���������������������� 94
Spheroid Bioprinting 96 Well����������������������������95
Spheroid Bioprinting 384 Well��������������������������95
Magnetic Levitation 6 / 24 Well������������������������96
Screening 96 / 384 Well�����������������������������������97
MagPen Single / 24 Well / 96 Well���������������������97
Consumables / Accessories Magnetic 3D Cell
Culture���������������������������������������������������������98/ ThinCert® Cell Culture Inserts for 6, 12 and24 Well Multiwell Plates������������������������ 99
ThinCert® Cell Culture Inserts 6 Well�������������� 100
ThinCert® Cell Culture Inserts 12 Well�������������101
ThinCert® Cell Culture Inserts 24 Well������������ 102
ThinCert® Plate 6 / 12 Well������������������������������ 102

www.gbo.com

/ 89

3D Cell Culture
CELLSTAR® Cell Culture Vessels Cell-Repellent Surface/

Forum No. 17:
CELLSTAR® Cell
Culture Vessels with
Cell-Repellent Surface(F073777)/

Application Report „Advantage of CELLSTAR®
Cell Culture Vessels
with Cell-Repellent
Surface for 3-D Cell
Culture in Hydrogels”(F073792)/

Spheroid and organoid culture/

Aggregation ofstem cells/

Suspension culture ofsemi-adherent/
adherent cell lines/

3D culture in hydrogels

CELLSTAR® CELL CULTURE VESSELS
CELL-REPELLENT SURFACE
Cell culture is an essential toolin drug discovery, tissue engineering, toxicology testing,stem cell research, as well as
in basic research.
Beside conventional two-dimensional (2D) monolayer cell
culture, 3D cell culture models are becoming a routine tool
which enables the expression of
extracellular matrix (ECM) components as well as the formation of cell-cell and cell-matrix
interactions. These characteristics are important for replicating

90 /www.gbo.com

in-vivo cell differentiation, proliferation, and function in vitro.
Greiner Bio-One developed
CELLSTAR ® cell culture vessels
with cell-repellent surface specifically for culturing in 3D. The
cell-repellent surface effectively prevents cell adherence andtherefore can promote the spontaneous formation of three-dimensional spheroids. Cell culture vessels with cell-repellent
surface are also ideal platforms for long-term cultivationin hydrogels.

3D Cell Culture
CELLSTAR® Cell Culture Vessels Cell-Repellent Surface

Cell Culture Dishes
Cell-Repellent Surface
Cell culture vessels with cell-repellent surface
reliably prevent cell attachment in suspension
cultures of semi adherent / adherent celllines where standard hydrophobic surfaces
generally used for suspension culture areinsufficient.

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Raw material: PS, Surface treatment: cell-repellent, Vent nock: yes, Sterile: +Item no.

HeightØ nominal size

Working volume

Total volume

SterileQty. inner / outer

627979

10 mm

35 mm

≤3 ml

10 ml

+

10 / 40628979

15 mm

60 mm

6 ml - 7 ml

28 ml

+

10 / 2066497020 mm

100 mm

16 ml - 17 ml

100 ml

+1/5

Cell Culture Flasks
Cell-Repellent Surface
A cell-repellent surface reliably prevent cellattachment in suspension cultures of semiadherent and adherent cell lines where
standard hydrophobic surfaces generally used
for suspension culture are insufficient.

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Raw material: PS, Surface treatment: cell-repellent, Sterile: +Item no.

Flask design

Cap colour

Total volume

Cap design

SterileQty. inner / outer

690980flat

l white50 ml

screw cap+

10 / 20690985flat

l white

50 ml

filter screw cap+

10 / 20658980flat

l white

250 mlscrew cap+

5 / 15658985flat

l white

250 mlfilter screw cap+

5 / 15660980flat

l white

550 mlscrew cap+

5/5660985flat

l white

550 mlfilter screw cap+

5/5

www.gbo.com

/ 91

3D Cell Culture
CELLSTAR® Cell Culture Vessels Cell-Repellent Surface

Item no.

Flask design

Cap colour

Total volume

Cap design

SterileQty. inner / outer

661980highl white

650 mlscrew cap+

4/4661985highl white

650 mlfilter screw cap+

4/4

Multiwell Plates / Microplates
Cell-Repellent Surface

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

6 / 12 / 24 / 48 well multiwell plates available/

96 / 384 well plates with various well geometries
and optional µClear® film bottom

nonpyrogenic

Raw material: PS, Surface treatment: cell-repellent, Sterile: +Well format

Well profile

BottomProductcolourTotal
volume(well)

Working
volume (well)

Lid

SterileQty. inner /outer6579706

F-bottomsolid

l clear16.1 ml

2 ml - 5 ml

yes, condensation rings+

1/5

66597012

F-bottomsolid

l clear

6.5 ml2 ml - 4 ml

yes, condensation rings+

1/5

66297024

F-bottomsolid

l clear

3.3 ml

0,5 ml 1,5 ml

yes, condensation rings+

1/5

67797048

F-bottomsolid

l clear

1.7 ml

0.5 ml - 1 ml

yes, condensation rings+

1/5

65097096

U-bottomsolid

l clear

323 µl

40 µl - 280 µl

yes, condensation rings+

1/6

65097996

U-bottomsolid

l clear

323 µl

40 µl - 280 µl

yes, condensation rings+

8 / 3265197096

V-bottomsolid

l clear234 µl

40 µl - 200 µl

yes, condensation rings+

1/6

65597096

F-bottom/ Chimney
Well

solid

l clear392 µl

25 µl - 340 µl

yes, condensation rings+

1/6

65597696

F-bottom/ Chimney
WellµClear®

l black

392 µl

25 µl - 340 µl

yes, condensation rings+

8 / 32655976-SIN

96

F-bottom/ Chimney
WellµClear®

l black

392 µl

25 µl - 340 µl

yes, condensation rings+

1 / 32781970384

F-bottomsolid

l clear131 µl

15 µl - 110 µl

yes+

1 / 60781974384

F-bottomµClear®

l white

131 µl

15 µl - 110 µl

yes+

8 / 32781976384

F-bottomµClear®

l black

131 µl

15 µl - 110 µl

yes+

8 / 32Item no.

92 /www.gbo.com

3D Cell Culture
CELLSTAR® Cell Culture Vessels Cell-Repellent Surface

Item no.

Well format

Well profile

BottomProductcolourTotal
volume(well)

Working
volume (well)

Lid

SterileQty. inner /outer781976-SIN

384

F-bottomµClear®

l black

131 µl

15 µl - 110 µl

yes+

1 / 32787979384

U-bottomsolid

l clear

122 µl

10 µl - 90 µl

yes+

8 / 32

SCREENSTAR Microplates
Cell-Repellent Surface

FREE OFdetectable
DNase

STERILE

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

For sophisticated microscopic applications andhigh content screening/

Highly transparent cycloolefin film bottom/

Adherent TC surface treatment

nonpyrogenic

Well format: 1536, Well profile: F-bottom, Bottom: Cycloolefin film, Raw material: COP, Surface treatment: cellrepellent, Lid: yes, Sterile: +Item no.

Product colour

Plate geometry

SterileQty. inner / outer

789979l black

LoBase+17 / 68782974l whiteHiBase+10 / 40

www.gbo.com

/ 93

3D Cell Culture
Magnetic 3D Cell Culture/

Brochure “3D Cell Culture” (F071076)/

For further information
please refer to our new
3D cell culture blog
series 3D Made Easy:/

3D in a 2D workflow/

Easy handling/

Fast 3D tissue
assembly/

No sample loss/

Scalable/

Ready for automation

MAGNETIC 3D CELL CULTURE
The core technology of Greiner
Bio-One’s magnetic 3D cell culture is the magnetisation of cellswith NanoShuttle-PL. The cells
can be aggregated with magnetic forces, either by levitation or
Bioprinting, to form structurally
and biologically representative
3D models in vitro. NanoShuttle-PL consists of gold, iron oxide, and Poly-L-Lysine. These
nanoparticles (Ø < 50 nm) magnetise cells by electrostatically attaching to cell membranes
during an overnight static incubation. Magnetised cells will ap-

94 /www.gbo.com

pear peppered with dark nanoparticles after incubation.
NanoShuttle-PL is biocompatible, having no effect on metabolism, proliferation and inflammatory stress. Additionally, it does
not interfere with experimental
techniques, such as fluorescence or Western blotting. With
magnetised spheroids, solution
addition and removal are madeeasy by using magnetic force tohold them in a stationary position during aspiration, thereby
limiting spheroid loss.

3D Cell Culture
Magnetic 3D Cell Culture

Spheroid Bioprinting
96 Well
Magnetized cells are brought together toform spheroids at the well bottom using weakmagnetic forces.

Item no.

Description

Content kit

Qty. inner / outer

65584096 Well Bioprinting Kit, clear

NanoShuttle-PL (3 vials), Spheroid Drive, Holding
Drive, 96 well cell culture microplates (clear) withcell-repellent surface (2 x 655970)

1/1

655841

96 Well Bioprinting Kit, black,µClear®

NanoShuttle-PL (3 vials), Spheroid Drive, Holding
Drive, 96 well cell culture microplates (black, µclear®)with cell-repellent surface (2 x 655976-SIN)

1/165585096 Well Ring Drive

96 Well Ring Drive for the formation of 3D ring
structures

1/1

65583096 Well Spheroid and Holding
Drive

Spheroid Drive, Holding Drive- /1

Spheroid Bioprinting
384 WellMagnetized cells are brought together toform spheroids at the well bottom using weakmagnetic forces.

Item no.

781840Description

Content kit

Qty. inner / outer

384 Well Bioprinting Kit, clear

NanoShuttle-PL (2 vials), Spheroid Drive, Holding
Drive, 384 well cell culture microplates (clear) withcell-repellent surface (2 x 781970)

1/1

www.gbo.com

/ 95

3D Cell Culture
Magnetic 3D Cell Culture

Item no.

Description

Content kit

Qty. inner / outer

781841384 Well Bioprinting Kit, black,µClear®

NanoShuttle-PL (2 vials), Spheroid Drive, Holding
Drive, 384 well cell culture microplates (black,
μClear®) with cell-repellent surface (2x 781976-SIN)

1/1781850384 Well Ring Drive

384 Well Ring Drive for the formation of 3D ring
structures

1/1

781830384 well spheroid and holding
drive

Spheroid Drive, Holding Drive- /1

Magnetic Levitation
6 / 24 Well
Magnetised cells are levitated off theplate bottom by a magnet and rapidly form
aggregates.

Item no.

96 /Description

Content kit

6578406 Well Bio-Assembler Kit

Levitation Drive, Holding Drive, NanoShuttle-PL(2 Vials), 6 well cell culture multiwell plates (2 x657970) and 6 Well Intermediate lid (2 x 657825)
with cell-repellent surface

657825

6 Well Intermediate lid

Intermediate lid with cell-repellent surface6578306 Well Levitation andHolding Drive

Levitation Drive (1), Holding Drive (1)- /166284024 Well Bio-Assembler Kit

Levitation Drive, Holding Drive, NanoShuttle-PL(2 Vials), 24 well cell culture multiwell plates (2 x662970) and 24 Well Intermediate lid (2 x 662825)
with cell-repellent surface

1/1

66282524 Well Intermediate lid

Intermediate lid with cell-repellent surface66283024 well Levitation andHolding Drive

Levitation Drive (1), Holding Drive (1)www.gbo.com

SterileQty. inner / outer

1/1
+

+

2 / 102 / 10- /1

3D Cell Culture
Magnetic 3D Cell Culture

Screening
96 / 384 Well
Ideal kits for combining applications andexamination methods.

Item no.

655846781846

Description

Content kit

Qty. inner / outer

96 Well BiO Assay Kit

NanoShuttle-PL (3 vials), 6 Well Levitation Drive, 96 Well
Spheroid, Holding and Ring Drive, 96 Well Deep Well Plate,
6 Well cell culture multiwell plates with cell-repellent surface(2 x 657970), 96 Well cell culture microplates (clear) withcell-repellent surface (2 x 655970), 6 Well Intermediate Lidwith cell-repellent surface (2 x 657825)

1/1

384 Well BiO Assay Kit

NanoShuttle-PL (2 vials), 6 Well Levitation Drive, 384 Well
Spheroid and Holding Drive, 96 Well Deep Well plate, 6 Well
cell culture multiwell plates with cell-repellent surface (2x 657970), 384 Well cell culture microplates (clear) withcell-repellent surface (2 x 781970), 6 Well Intermediate Lidwith cell-repellent surface (2 x 657825)

1/1

MagPenSingle / 24 Well / 96 Well
MagPen is a smart assistant for easy and fasttransfer and collection of magnetized cell
cultures by a simple “pick up-and-drop”-step.

Item no.

Description

Content kit

Sterile657850MagPen 3-packTeflon caps (3), magnets (3)- /365782424 Well Multi-MagPen Kit

24 Well Multi-MagPen Drive and 24 Well Multi-MagPen Sleeve (2 x 651524) with cell-repellent surface- /165152424 Well Multi-MagPen
SleeveMulti-MagPen Sleeve with cell-repellent surface

657896

96 Well Multi-MagPen Kit

96 Well Multi-MagPen Drive und 96 Well Multi-MagPen Sleeve (2 x 61596) with cell-repellent surface+

Qty. inner / outer1 / 10- /1

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/ 97

3D Cell Culture
Magnetic 3D Cell Culture

Item no.

651596

Description

Content kit

SterileQty. inner / outer

96 Well Multi-MagPen
SleeveMulti-MagPen Sleeve with cell-repellent surface+

1 / 10Consumables / Accessories
Magnetic 3D Cell Culture/

Item no.

98 /NanoShuttle-PL consists of gold, iron oxide andPoly-L-Lysine

Description

Content kit

Qty. inner / outer

657841

NanoShuttle-PL

600 μl vials of NanoShuttle-PL (1)- /1657843

NanoShuttle-PL 3-pack

600 μl vials of NanoShuttle-PL (3)- /3657846

NanoShuttle-PL 6-pack

600 μl vials of NanoShuttle-PL (6)- /6657852

NanoShuttle-PL 12-pack

600 μl vials of NanoShuttle-PL (12)- /12www.gbo.com

3D Cell Culture
ThinCert® Cell Culture Inserts for 6, 12 and 24 Well Multiwell Plates/

Forum No. 8: ThinCert ®
cell culture products –Overview (F073017)/

Application Note
„Immuno cytochemistry“(F073100)/

For further information
and application notes
please refer to our
website: www.gbo.com

THINCERT® CELL CULTURE INSERTS
FOR 6, 12 AND 24 WELL MULTIWELL PLATES
For advanced cell and tissue culture applications,
Greiner Bio-One offers a broad
range of membrane supports- ThinCert®.

/

Stable clear
polystyrene housing/

Sealed PET capillarypore membrane/

Pre-configured
multiwell plates with
ThinCert ® cell culture
inserts available on
requestCombining 6 different membrane specifications (pore sizeand density) in geometries tofit 6, 12 and 24 well plates, theThinCert® cell culture inserts
are suitable for a wide range ofapplications including transport,secretion and diffusion studies,migrational experiments, cytotoxicity testing, co-cultures,trans epithelial electric resist-ance (TEER) measurements, aswell as primary cell culture.
ThinCert ® cell culture inserts
are compatible with standard
CELLSTAR ® multiwell plates
from Greiner Bio-One, and are
pre-packed together with therequisite number of plates. The
automated production processincludes double optical controlof each insert produced, ensuring that any biological contamination is avoided. The sterility ofthe single blisterpacked inserts
and multiwell plates is ensured
by irradiation.

www.gbo.com

/ 99

3D Cell Culture
ThinCert® Cell Culture Inserts for 6, 12 and 24 Well Multiwell Plates

ThinCert® Cell Culture Inserts
6 WellSTERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Hanging geometry/

Improved cell adhesion through physical surface
treatment/

Simplified pipetting due to self-lift geometry/

Enhanced pipetting access and gas exchange

nonpyrogenic

Feature: 4 multiwell plates / box, Height: 16.25 mm, Ø internal: 24.85 mm, Ø external: 27.85 mm,
Cultural surface: 452.4 mm², Surface treatment: TC, Working volume (ThinCert®): 1 ml - 3.6 ml,
Working volume (well): 2 ml - 4.15 ml, Sterile: +Item no.

100 /Pore density

Ø poreOptical membrane properties

SterileQty. inner / outer

6576401 x 10⁸ / cm²

0.4 µmtranslucent+

1 / 246576412 x 10⁸/ cm²

0.4 µmclear+

1 / 246576102 x 10⁸/ cm²

1 µm

clear

+

1 / 246576300,6 x 10⁶ / cm²

3 µm

clear

+

1 / 246576312 x 10⁸/ cm²

3 µm

translucent+

1 / 24657638

0,15 x 10⁶ / cm²

8 µm

translucent+

1 / 24www.gbo.com

3D Cell Culture
ThinCert® Cell Culture Inserts for 6, 12 and 24 Well Multiwell Plates

ThinCert® Cell Culture Inserts
12 Well

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Hanging geometry/

Improved cell adhesion through physical surface
treatment/

Simplified pipetting due to self-lift geometry/

Enhanced pipetting access and gas exchange

nonpyrogenic

Feature: 4 multiwell plates / box, Height: 16.25 mm, Ø internal: 13.85 mm, Ø external: 15.85 mm,
Cultural surface: 113.1 mm², Surface treatment: TC, Working volume (ThinCert®): 0.3 ml - 1 ml,
Working volume (well): 1 ml - 2 ml, Sterile: +Item no.

Pore density

Ø poreOptical membrane properties

SterileQty. inner / outer

6656401 x 10⁸ / cm²

0.4 µmtranslucent+

1 / 486656412 x 10⁸/ cm²

0.4 µmclear+

1 / 486656102 x 10⁸/ cm²

1 µm

clear

+

1 / 486656300,6 x 10⁶ / cm²

3 µm

clear

+

1 / 486656312 x 10⁸/ cm²

3 µm

translucent+

1 / 48665638

0,15 x 10⁶ / cm²

8 µm

translucent+

1 / 48www.gbo.com

/ 101

3D Cell Culture
ThinCert® Cell Culture Inserts for 6, 12 and 24 Well Multiwell Plates

ThinCert® Cell Culture Inserts
24 Well

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Hanging geometry/

Improved cell adhesion through physical surface
treatment/

Simplified pipetting due to self-lift geometry/

Enhanced pipetting access and gas exchange

nonpyrogenic

Feature: 2 multiwell plates / box, Height: 16.25 mm, Ø internal: 8.4 mm, Ø external: 10.4 mm,
Cultural surface: 33.6 mm², Surface treatment: TC, Working volume (ThinCert®): 0.1 ml - 0.35 ml,
Working volume (well): 0.4 ml - 1.2 ml, Sterile: +Item no.

Pore density

Ø poreOptical membrane properties

SterileQty. inner / outer6626401 x 10⁸ / cm²

0.4 µmtranslucent+

1 / 486626412 x 10⁸/ cm²

0.4 µmclear+

1 / 486626102 x 10⁸/ cm²

1 µm

clear

+

1 / 486626300,6 x 10⁶ / cm²

3 µm

clear

+

1 / 486626312 x 10⁸/ cm²

3 µm

translucent+

1 / 48662638

0,15 x 10⁶ / cm²

8 µm

translucent+

1 / 48

ThinCert® Plate
6 / 12 Well

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Optimised for use with ThinCert® cell culture
inserts/

Deep wells for an increased volume of medium in
air-lift culture/

Notches for fixed insert position/

Available in 6 and 12 well format

nonpyrogenic

Height: 39.5 mm, Length: 129.5 mm, Width: 86.6 mm, Lid: yes, condensation rings, Sterile: +Item no.

657110102 /www.gbo.com

Well format

Working volume (well)

SterileQty. inner / outer6≤20 ml

+1 / 503D Cell Culture
ThinCert® Cell Culture Inserts for 6, 12 and 24 Well Multiwell Plates

Item no.

665110Well format

Working volume (well)

SterileQty. inner / outer

12

≤4 ml

+

1 / 60www.gbo.com

/ 103

Microscopy is a basic and important method, which is often used inresearch as well as in medical diagnostics. For this purpose, Greiner
Bio-One offers a variety of tailor-made solutions, which guarantee
optimal basic conditions for microscopic experiments.

104 /www.gbo.com

Products for Microscopy
﻿

PRODUCTS FOR MICROSCOPY/ CELLview Dish Cell Culture Dish with
Glass Bottom���������������������������������������110
CELLview Dish ������������������������������������������������111/ CELLview Slide Cell Culture Slide with
Glass Bottom���������������������������������������112
CELLview Slide ���������������������������������������������� 113/ CELLview Plate Cell Culture Plate with
Glass Bottom���������������������������������������114
CELLview Plate ���������������������������������������������� 115/ SCREENSTAR Microplates �������������������116
SCREENSTAR Microplates 96 / 384 /
1536 Well��������������������������������������������������������� 117/ SensoPlate Glass Bottom Microplates��118
SensoPlate Glass Bottom Plates 24 /96 /384 / 1536 Well������������������������������������������������ 119

www.gbo.com

/ 105

Products for Microscopy
﻿

PRODUCTSFORMICROSCOPY

Well distance equal to a
96 well microplate

Positioning notch
for automatedmicroscopy in well A1

0.17 mm borosilicate glass

Detachable black upper housing
10 wells withalphanumeric coding

106 /www.gbo.com

Products for Microscopy
﻿

PRODUCTS FOR MICROSCOPYFEATURES:
Technological progression in
confocal microscopy, opticalsystems and emerging technologies continues to elevate microscopy as one of the mostpowerful tools in cell biology.
With its advantages for molecular selectivity and capabilityof live observation, fluorescencemicroscopy currently is among
the most widely used approaches for high-resolution, non-invasive imaging of living cells.
Depending on the complexity of live cell imaging experiments and the requirements ofthe corresponding microscope,
the requirements for the utilised
disposables can be as comprehensive. Greiner Bio One’s imaging consumables are tailoredsolutions to provide optimal
basic settings for your microscopic experiment.

/ Maximal light transmission/ Innovative designfor maximal
planarity/ Reduced meniscus
effect/ Optimal cell attachment and viability

CELLview Products
Tailored solutions
to provide optimal
basic settings foryour microscopic
experimentwww.gbo.com

/ 107

Products for Microscopy﻿

CELLview products combine the convenience of a plastic disposable with the highoptical quality of a 0.17 mm thincover glass bottom, providing
superior high-resolution microscopic images of in-vitro cultures. The specific design with theembedded cover glass bottom
guarantees a single-plane, flatbottom with a consistent working distance, maximal planarityand optimal thermal conductivity in heated platforms.

108 /www.gbo.com

SCREENSTAR microplateswith a 0.19 mm cycloolefin film
bottom are suitable for sophisticated microscopic applications,in high content screening (HCS)or high-resolution microscopy
with water and oil immersion
objectives. Cycloolefins possess
excellent optical features anddisplay a low background in thelower UV, with a refractive index
and focus background comparable to glass.

Products for Microscopy
﻿

SCREENSTARThe optimised
microplate geometry
and the recessedbottom facilitate
imaging of all peripheral wells even withimmersion objectivesµClear ® film bottom plateswith a 0.19 mm polystyrene film
bottom can also be applied for
microscopic applications,as the thickness fits into thetolerance window of mostmicroscopic objectives anddoes not require any special

adaptations or corrective lenses. Depending on the wavelength of the analysed probes
excellent images can be achieved due to Greiner Bio One`s optical quality of the polystyrene
film bottom.

CELLview Products

SCREENSTAR Microplates

Dishes, slides and plateswith 0.17 mm thin cover glassbottom for superior highresolution microscopic
images of in-vitro cultures.

96 / 384 / 1536 well plates witha 0.19 mm thin cycloolefin
film bottom for sophisticatedmicroscopic applications,in high content screening or
high-resolution microcopy.

SensoPlate Glass Bottom
PlatesGlass bottom plates without
surface treatment for
fluorescence correlation
spectroscopy and microscopic applications.

www.gbo.com

/ 109

Products for Microscopy
CELLview Dish Cell Culture Dish with Glass Bottom/

Application Note
„Protein localisation
using confocal laser
scanning microscopy“(F073101)/

Application Note
„Live cell imaging on
Golgi morpholgy using
the CELLview dish“(F074048)/

TC and Advanced TC
surface modification
available/

Maximal spectral
transmission/

No autofluorescence,no depolarisation oflight

CELLVIEW DISHCELL CULTURE DISH WITH GLASS BOTTOM
CELLview Dish combines theconvenience of a standard size
35 mm disposable plastic cell
culture dish with the opticalquality of glass, providing superior high-resolution microscopic images of in-vitro cultivated cultures.
It is made from high-grade polystyrene combined with an integrated glass bottom. The innovative design of the dish provides
a single-plane, flat bottom witha consistent working distanceand maximal planarity. Moreover, the dish bottom configuration facilitates optimal thermal
conductivity and avoids thermalvariations in heated platforms

110 /www.gbo.com

used for live cell imaging.
The subdivided version of
CELLview Dish enables simultaneous multiplex analyses ofdifferent cell lines, various stimulations or diverse transfections. Quartering the cell culture
dish provides four individualcompartments with a growtharea of approximately 1.9 cm²,
allowing minimisation of cells
and reagents required per individual assay. In addition to theuntreated glass surface, Greiner
Bio-One provides a tissue culture surface as well as the Advanced TC surface modification
to enhance the attachment ofadherent cells, thus eliminating
the need for protein coating.

Products for Microscopy
CELLview Dish Cell Culture Dish with Glass Bottom

CELLview Dish
The CELLview cell culture dish combines
the convenience of a standard size 35 mm
disposable plastic cell culture dish with theoptical quality of glass, providing superior
high-resolution microscopic images of in-vitro
cultivated cultures.
/

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

Embedded glass bottom for maximal planarity

nonpyrogenic

Ø: 35 mm, Bottom: glass, Vent nock: yes, Sterile: +Compartments

Growtharea

627861

1627860Growtharea / unit

Total
volume(well)

Surface treatment

Working
volumeTotal
volume8.7 cm²

untreated

2.5 ml 5 ml

1

8.7 cm²

TC6279651

8.7 cm²

Advanced TC

6278714

1.9 cm²

untreated

1.5 ml

6278704

1.9 cm²

TC

6279754

1.9 cm²

Advanced TC

Item no.

Working
volume (well)

SterileQty. inner /
outer

10 ml

+

10 / 40

2.5 ml 5 ml

10 ml

+

10 / 40

2.5 ml 5 ml

10 ml

+

10 / 400.1 ml 0.5 ml+

10 / 40

1.5 ml0.1 ml 0.5 ml+

10 / 40

1.5 ml0.1 ml 0.5 ml+

10 / 40/ Application Note „Protein localisation using confocal laser scanning microscopy“ (F073101)/ Application Note „Live cell imaging on Golgi morpholgy using the CELLview dish“ (F074048)

www.gbo.com

/ 111

Products for Microscopy
CELLview Slide Cell Culture Slide with Glass Bottom/

For further information, please refer to our
website: www.gbo.com/

10 wells with alphanumeric coding/

Black detachable
compartmentalization/

Well distance is equal
to a 96 well microplate/

Positioning notch forautomated microscopy/

Highly transparent
achromatic borosilicate glass bottom

CELLVIEW SLIDECELL CULTURE SLIDE WITH GLASS BOTTOM
CELLview Slide consists of atransparent slide with a black
upper housing that effectively subdivides the slide into 10
compartments, which have been
designed to mimic the size andlayout of a standard 96 well microplate. Because of this standard layout, the slide is compatible with multichannel pipettes
making it simple and efficient
to use. Furthermore, the round
well design helps to reduce meniscus effects for optimum results in cell culture and microscopic analysis. The slide has a
0.17 mm thin cover glass embed-

112 /www.gbo.com

ded in its bottom for improved
optical clarity and imaging.
Embedding the cover glass
guarantees an even focal plane
which is a prerequisite for all
high-speed and high-resolution
microscopy applications. Furthermore, the black upper housing reduces cross talk between
adjacent wells during fluorescence microscopy and the optical glass, which exhibits virtuallyno autofluorescence, allows for
maximum spectral transmission
without depolarisation of transmitted light.

Products for Microscopy
CELLview Slide Cell Culture Slide with Glass Bottom

CELLview Slide

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

10 wells with alphanumeric coding/

Positioning notch for automated microscopy/

Black detachable compartmentalization/

Reduced meniscus effect due to round well design/

Glass thickness: 0.17 mm

nonpyrogenic

Well format: 10, Height: 12.6 mm, Length: 75 mm, Width: 25 mm, Growth area / unit: 34 mm², Bottom: glass,Total volume (well): 440 µl, Sterile: +Item no.

Surface treatment

SterileQty. inner / outer

543078TC+

1 / 20543079TC+

5 / 45543978Advanced TC+

1 / 20543979Advanced TC+

5 / 45/ Sample packs are available on request.

www.gbo.com

/ 113

Products for Microscopy
CELLview Plate Cell Culture Plate with Glass Bottom/

For plates with an
optical cycloolefin film
bottom, please refer
to our SCREENSTAR
plates in this chapter.

/

Outstanding image
quality and resolution/

Cycloolefin-based
frame with 0.17 mm
glass bottom/

Excellent opticaltransparency/

Recessed well bottom
facilitating the use oflenses with low working
distance and highaperture/Compatible withadvanced confocal
microscopic systems

CELLVIEW PLATE
CELL CULTURE PLATE WITH GLASS BOTTOM
CELLview glass bottom microplates are designed for demanding and high-resolution
microscopic applications.
They consist of a cycloolefinbased black frame with a 0.17
mm thin borosilicate glass bottom providing superior imagesof in-vitro cultures. The optimised microplate geometry and114 /www.gbo.com

the recessed bottom facilitate
imaging of all peripheral wells
even with immersion objectives.
The round conical well design
reduces the meniscus effect inorder to assure equal cellular
distribution and constant imaging results. An appropriate surface treatment improves cellular
attachment and growth.

Products for Microscopy
CELLview Plate Cell Culture Plate with Glass Bottom

CELLview PlateSTERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

For outstanding image quality and resolution/

Recessed well bottom facilitating the use of objectives with low working distance/Ditch at the perimeter can be filled with liquid tominimise edge effects and evaporation/

Compatible with advanced automated microscopic
systems

nonpyrogenic

Well format: 96, Growth area / unit: 33 mm², Well profile: F-bottom / Chimney Well, Bottom: glass, Raw material: COP,
Working volume (well): 25 µl - 440 µl, Lid: yes, Sterile: +Item no.

Surface treatment

Product colour

SterileQty. inner / outer655891TC

l black+

1 / 16655981

Advanced TC

l black+

1 / 16www.gbo.com

/ 115

Products for Microscopy
SCREENSTAR Microplates/

Forum No. 15:
SCREENSTAR: A 1536
Well Microplate forHigh-Content and HighThroughput Screening(F073120)/

96 / 384 / 1536 wellformat/For sophisticatedmicroscopic applications and high content
screening/

Highly transparent
cycloolefin film bottom/

Adherent TC surface
treatment

SCREENSTAR MICROPLATESSCREENSTAR are specialised
microplates for sophisticatedmicroscopic applications, inhigh content screening (HCS)or high-resolution microscopy
with water and oil immersion
objectives.
They combine outstanding
glass-like optical properties
with an excellent surface foradherent cell culture.
Moreover, the plates display ex-

116 /www.gbo.com

cellent optical properties with
reduced autofluorescence inthe lower UV range, low birefringence and a refractive index
of 1.53 comparable to glass.
SCREENSTAR microplates enable complete periphery accessfor high magnification objectives. They are entirely manufactured out of cycloolefin witha black pigmented cycloolefin
frame and a 190 μm ultraclear
cycloolefin film bottom.

Products for Microscopy
SCREENSTAR Microplates

SCREENSTAR Microplates
96 / 384 / 1536 Well

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

For sophisticated microscopic applications andhigh content screening/

Highly transparent cycloolefin film bottom/

Adherent TC surface treatment

nonpyrogenic

Bottom: Cycloolefin film, Raw material: COP, Surface treatment: TC, Sterile: +Item no.

Well format

Growth area
/ unitWell profile

Working volume (well)

Lid

SterileQty. inner /outer65586696

33 mm²

F-bottom /Chimney Welll black

25 µl - 440 µl

yes+

1 / 16781866384

8.1 mm²

F-bottoml black

10 µl - 110 µl

yes+

8 / 327898661536

2.1 mm²

F-bottoml black

3 µl - 15 µlno+17 / 68

Product colour/ Forum No. 15: SCREENSTAR: A 1536 Well Microplate for High-Content and High-Throughput Screening (F073120)

www.gbo.com

/ 117

Products for Microscopy
SensoPlate Glass Bottom Microplates/

For further information, please refer to our
website: www.gbo.com/

For fluorescencecorrelation spectroscopy and microscopic
applications/

24 / 96 / 384 / 1536 wellformat/Black frame with highly
transparent glassbottom/Glass bottom thicknessof 175 µm is equivalent
to the light path ofstandard coverslips/

Sterile, with lid andsingle-packed

SENSOPLATEGLASS BOTTOM MICROPLATES
The research of biomolecular
processes on the level of single molecules and in volume ranges equivalent to the size of asingle bacterium is of immense importance, both in basic
research and in industrial highthroughput screening. The
combination of modern confocal optics, new fluorescent dyes,
sensitive photomultipliers andimproved data processing has
revolutionised the technique offluorescence correlation spectroscopy (FCS).
Over the past few years this has
led to its widespread application,

118 /www.gbo.com

and alongside the technologicaladvances in hardware development, Greiner Bio-One worked
hand-in-hand with customers
and instrument suppliers to develop the glass bottom microplates. These better satisfy therequirements of fluorescencecorrelation spectroscopy withregards to optical clarity anddeformation when compared
to standard polystyrene plates.
The SensoPlate family was developed in a complete productline consisting of 24, 96, 384 and
1536 well glass bottom formats.

Products for Microscopy
SensoPlate Glass Bottom Microplates

SensoPlate Glass Bottom Plates
24 /96 / 384 / 1536 Well/

For fluorescence correlation spectroscopy andmicroscopic applications/

24 / 96 / 384 / 1536 well format/

Black frame with highly transparent glass bottom/

Glass bottom thickness of 175 µm is equivalent to
the light path of standard coverslips/

Sterile, with lid and single-packed

STERILE

Well profile: F-bottom, Bottom: glass, Raw material: PS, Surface treatment: untreated, Lid: yes, Sterile: +Item no.

Well format

Product colour

Plate geometry

Working volume (well)

SterileQty. inner / outer66289224

l black

0,5 ml - 1,5 ml+

1 / 1265589296l black

25 µl - 340 µl+

1 / 16781892384

l black

10 µl - 130 µl+

1 / 167838921536

l black

3 µl - 10 µl+

1 / 16

LoBasewww.gbo.com

/ 119Continued progress in research and related technologies, such as
microscopy, imaging, detection and liquid handling, has given rise toa wide variety of platforms used in basic science, biotechnology andpharmaceutical drug development. Today, researchers can selectapplication-specific microplates among a broad range of productsthat differ in format, design, base material, colour and surface
properties.

120 /www.gbo.com

HTS-Microplates﻿

HTS-MICROPLATES/ 96 Well Microplates ���������������������������� 126/ UV-Star® Microplates ������������������������� 146

96 Well Microplates Polystyrene���������������������127

UV-Star® Microplates �������������������������������������147

96 Well Microplates Polystyrene - Half Area��� 128
96 Well Microplates Polypropylene���������������� 129/ Lids / Sealers / CapMats ��������������������� 148
Lids ��������������������������������������������������������������� 149/ 384 Well Microplates ��������������������������130

Sealers ��������������������������������������������������������� 149

384 Well Microplates Polystyrene������������������� 131

CapMats �������������������������������������������������������� 150

384 Well Microplates Polystyrene Small Volume������������������������������������������������� 132
384 Well Microplates Polypropylene�������������� 132/ Protein Crystallisation Plates ��������������151
96 Well CrystalQuick /CrystalQuick Plus Plates�������������������������������� 152/ 1536 Well Microplates ������������������������� 134
1536 Well Microplates ������������������������������������ 135

24 Well ComboPlate CrystalBridge /Coverslips����������������������������������������������������� 152/ Polypropylene Storage Plates ������������� 136
96 Well MASTERBLOCK® Polypropylene����������137
96 Well Storage Box ���������������������������������������137
384 Deep Well MASTERBLOCK®
Polypropylene������������������������������������������������ 138
1536 Deep Well Microplates Polypropylene���� 138/ Microplates for Compound Storage ���� 139
Microplates for Compound Storage 384
and 1536 Well������������������������������������������������ 140/ Non-binding Microplates ���������������������141
Non-binding Microplates 96 Well������������������� 142
Non-binding Microplates 384 and 1536 Well��� 142/ Streptavidin-coated Microplates���������144
Streptavidin-coated Microplates 96
and 384 Well�������������������������������������������������� 145

www.gbo.com

/ 121

HTS-Microplates﻿

MICROPLATES/ Comprehensive microplate
portfolio/ 96 / 384 / 1536 Well
/ High-quality materials for eachapplication/ Different well geometries/ Various surface modifications

122 /www.gbo.com

HTS-Microplates﻿

HIGH-THROUGHPUT SCREENING
MICROPLATES FROM GREINER BIO-ONE
Polypropylene (PP) and polystyrene (PS) are the standard
materials used to manufacture the majority of microplates.
Polystyrene is a highly clear polymer with excellent optical properties which makes it ideal for
precise optical measurements.
Polystyrene is also characterised by its ability to bind biomolecules, such as proteins, and
it is therefore often used for
manufacturing immunologicalproducts. Polystyrene is suitable for work with cell cultures.

Polypropylene is characterised by its excellent chemical and thermal stability. It is
the ideal polymer for storage
vessels or microplates. In addition, Greiner Bio-One manufactures microplates with special requirement profiles, suchas the UV-Star® or SCREENSTAR
microplates made from different
cycloolefins. They are characterised by their low level of autofluorescence, exceptionallyhigh clarity, especially in the
UV range, and greater chemical stability when compared with
polystyrene.

GREINER BIO-ONE
MICROPLATES:/ Manufactured
under DIN ISO 9001
guidelines/ Full traceability/ Footprint compatible with automatedsystems/ Free of detectableendotoxins/ Free of detectable
DNase, RNase andhuman DNA
/ Available with barcode on request

OUR PORTFOLIO
OFFERS THE
RIGHT PLATFORM
AND COATINGFOR EVERYAPPLICATION

www.gbo.com

/ 123

HTS-Microplates﻿µClear® and UV-Star®
Film Bottom Microplates
The development of a new andpatented processing technique has made it possible for
us to produce microplates withultra-thin films, without theuse of adhesives or solvents –the µClear® and UV-Star® products. This special method eradicates the risk of leaking wells.
SensoPlate Glass Bottom
Microplates
SensoPlate glass bottom
microplates consist of a black
pigmented polystyrene frameon to which a 175 μm thick borosilicate glass bottom is bonded.
Thanks to the high optical qualityof the glass bottom as well as theminimal bending, SensoPlate are

124 /www.gbo.com

especially recommended for
fluorescence correlation spectroscopy and sophisticatedmicroscopic applications.
Black or White?
White microplates are usually used for luminescence measurements (e. g. Luciferase
Reporter Assays) and black
microplates for fluorescence. measurements (e.g. Green
Fluorescence Protein). The critical properties in these methods, such as background, autofluorescence or crosstalk areconsiderably improved by theuse of black or white pigmented microplates.

Further information on
microplates:
Microplate Selection
Guide (F073048)
Microplate Dimensions
Guide (F073027) .

HTS-Microplates﻿

BARCODE:You can download
the microplate orderform directly from our
website www.gbo.com(Item no. F073010)

MICROLON, FLUOTRAC, LUMITRAC

Non-binding Surfaces

These brands stand for the quality of ourimmunology products. MICROLON are clear
microplates for transmission measurements. FLUOTRAC are black microplates
for fluorescence measurements. LUMITRAC
are white microplates for luminescencemeasurements.

Non-binding surfaces are characterised bytheir low binding capacity for biomolecules
such as DNA, RNA, peptides and proteins.
The repellent property of the non-binding
surfaces for biomoleculescan be advantageous in biochemical assays by increasing
the sensitivity, reducing the background andimproving the signal-to-noise ratio.

MICROLON, FLUOTRAC,
LUMITRAC
Quality brands for immunological Plates:
MICROLON
clear microplates fortransmission measurements

Non-binding surfaces

Cell Culture Surface

Low binding capacity forbiomolecules such as DNA,
RNA, peptides and proteinsfor sensitive biochemical
assaysSignificantly improves theadhesion of cells and thebinding of proteins to theplastic surface(see chapter cell culture)

FLUOTRAC
black microplates forfluorescence measurements
LUMITRAC
white microplates forluminescence measurementswww.gbo.com

/ 125

HTS-Microplates
96 Well Microplates/

For details regarding
the different well profiles, please refer to thetechnical appendix.

/

Available inpolystyrene,
polypropylene andcycloolefin/

Clear / black / white/With U-bottom,
V-bottom or F-bottom/

Sterile / non-sterile/

Non-treated or inhigh-binding quality/

In non-binding quality

96 WELL MICROPLATES
Since its introduction in the
1960’s applications for the 96
well microplate have continually
increased to the extent that it is
impossible to envisage modern
research and industry without ittoday. Greiner Bio-One has been
manufacturing microplates andstrip microplates for diagnostics

126 /www.gbo.com

and immunological research forover 40 years. A large number ofdifferent 96 well microplates isavailable in a wide variety of surface treatments. The spectrum
ranges from clear bottom microplates and completely black orwhite microplates to UV-Star®
products.

HTS-Microplates
96 Well Microplates

96 Well Microplates
Polystyrene

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

With U-bottom, V-bottom or F-bottom/

Sterile / non-sterile/

Non-treated or in high-binding quality/

With solid bottom or µClear® film bottom

nonpyrogenic

Well format: 96, Raw material: PS, Lid: no
Surfacetreatment

ProductcolourWorking
volume(well)

solid

untreatedl clear

40 µl 280 µl

U-bottom

solid

untreatedl clear

40 µl 280 µl

651101

V-bottom

solid

untreatedl clear

40 µl 200 µl

651161

V-bottom

solid

untreatedl clear

40 µl 200 µl

655101

F-bottom

solid

untreatedl clear

25 µl 340 µl

655161

F-bottom

solid

untreatedl clear

25 µl 340 µl+

2 / 100

655161

F-bottom

solid

untreatedl clear

25 µl 340 µl+

2 / 100

655075F-bottom /Chimney Wellsolid

untreated

l white

25 µl 340 µl

655074

F-bottom /Chimney Wellsolid

highbinding

LUMITRAC
600

l white

25 µl 340 µl+

10 / 40655077

F-bottom /Chimney Wellsolid

highbinding

FLUOTRAC
600

l black

25 µl 340 µl+

10 / 40655076

F-bottom /Chimney Wellsolid

untreatedl black

25 µl 340 µl

10 / 40655095F-bottom /Chimney Well

µClear®

untreated

l white

25 µl 340 µl

10 / 40655094

F-bottom /Chimney Well

µClear®

highbinding

l white

25 µl 340 µl+

10 / 40655097

F-bottom /Chimney Well

µClear®

highbinding

l black

25 µl 340 µl+

10 / 40655096

F-bottom /Chimney Well

µClear®

l black

25 µl 340 µl

Item no.

Well profile

Bottom

650101

U-bottom

650161

Bindingcharacterisitc

Binding brandnameuntreated

SterileQty. inner /outer

10 / 100+

2 / 100
10 / 100+

2 / 100
10 / 100

10 / 40

10 / 40

www.gbo.com

/ 127

HTS-Microplates
96 Well Microplates

96 Well Microplates
Polystyrene - Half Area

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Sterile / non-sterile/

Reduction of sample volume by up to 50 %/Standardised pathlength (1 cm=170 µl, 0.5 cm=80 µl)/

Non-treated or in high-binding quality/

With solid bottom or µClear® film bottom

nonpyrogenic

Well format: 96, Well profile: F-bottom, Raw material: PS, Plate design: half area,
Working volume (well): 15 µl - 175 µl, Lid: no

Item no.

BottomBinding characterisitc

Surface treatment

Product colour

SterileQty. inner / outer+

10 / 40675161solid

untreatedl clear675101solid

untreatedl clear675074solid

675075solid

675077solid

675076solid

untreatedl black

10 / 40675096µClear®

untreatedl black

10 / 40high-binding

l whiteuntreated
high-binding

10 / 40+l whitel black

10 / 40
10 / 40+

10 / 40/ Further information on Half Area Microplates: Forum No. 16: 96 Well Half Area Microplates and their Application in Fluorescence, Luminescenceand Transmission Measurements (F073121)

128 /

www.gbo.com

HTS-Microplates
96 Well Microplates

96 Well Microplates
Polypropylene

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Ideally suited for the storage of active agents,
DNA/RNA or stock cultures/

High chemical resistance and temperature
tolerance/

Black plates for fluorescence polarisation

nonpyrogenic

Well format: 96, Bottom: solid, Raw material: PP, Surface treatment: untreated, Lid: no

Item no.

Well profile

Product colour

Working volume (well)

SterileQty. inner / outer

650201

U-bottom / Chimney Welll natural

50 µl - 300 µl

650261

U-bottom / Chimney Welll natural

50 µl - 300 µl

650209

U-bottom / Chimney Welll black

50 µl - 300 µl

10 / 100

655201

F-bottom / Chimney Welll natural

25 µl - 370 µl

10 / 100

655209

F-bottom / Chimney Welll black

25 µl - 370 µl

10 / 100

651201

V-bottom/Chimney Welll natural

50 µl - 335 µl

10 / 100

651209V-bottom/Chimney Welll black

50 µl - 335 µl

10 / 100

10 / 100+

10 / 100

www.gbo.com

/ 129

HTS-Microplates
384 Well Microplates/

For cell culture treatedmicroplates, pleaserefer to chapter cell
culture/Available in polystyrene, polypropylene andcycloolefin/

Clear / black / white/With F-bottom, V-bottom or as Small Volume
option/Solid bottom or µClear ®
film bottom/

Non-treated or in highbinding quality/

In non-binding quality

384 WELL MICROPLATESDrug screening has undergone rapid development over the
past years.
The number of tests with newtargets and the number of active
agents to be tested is constantly increasing. Volume reduction,
simple testing and cost savings
are some of the highest priorities and high-format microplates with a low well volume are
one of the most important toolsin achieving this. One of the first

130 /www.gbo.com

higher-format microplates was
the 384 well plate, launched byGreiner Bio-One in 1994/1995.
Compared with the 96 well microplate, the number of wells
is quadrupled in this microplate, combined with a volume reduction from 382 µl to 131 µl. The
well-to-well spacing is 4.5 mm(96 well plate: 9 mm). The external dimensions of the 384 well
microplates are compatible with
automated systems.

HTS-Microplates
384 Well Microplates

384 Well Microplates
Polystyrene

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Solid bottom or µClear ® film bottom/

Sterile / non-sterile/

Non-treated or in high-binding quality

nonpyrogenic

Well format: 384, Well profile: F-bottom, Raw material: PS, Working volume (well): 15 µl - 110 µl

Item no.

BottomBinding characterisitc

Binding brand nameSurface treatment

SterileQty. inner /outer

Product colourLidl clearnol clearno+10 / 40

781101solid

781061solid

781162solid

untreatedl clearno+10 / 100

781185solid

untreatedl clearyes+

1 / 32781186solid

untreatedl clearyes+

8 / 32781074solid

l whiteno+10 / 40781075solid

l whiteno781077solid

l black

no781076solid

untreatedl blackno10 / 40781095µClear®

untreated

l whiteno10 / 40781097µClear®

l black

no781096µClear®

l black

nountreated
high-binding

high-binding

LUMITRAC 600
untreated

high-binding

FLUOTRAC 600

high-binding
untreated

10 / 100

10 / 40++

10 / 40

10 / 40
10 / 40

www.gbo.com

/ 131

HTS-Microplates
384 Well Microplates

384 Well Microplates
Polystyrene - Small Volume

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Perfect for top reading even at low working
volumes/

Savings in reagent similar to 1536 well microplates/

Made of clear / black / white polystyrene fortransmission, fluorescence or luminescencemeasurements

nonpyrogenic

Well format: 384, Well profile: F-bottom, Bottom: solid, Raw material: PS, Surface treatment: untreated, Plate
geometry: HiBase, Plate design: Small Volume, Working volume (well): 4 µl - 25 µl, Lid: no

Item no.

Product colour

Qty. inner / outer

784101l clear10 / 40784075l white10 / 40784075-25

l white25 / 150

784076l black

10 / 40784076-25

l black

25 / 150

384 Well Microplates
Polypropylene

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Ideally suited for the storage of active agents,
DNA/RNA or stock cultures/

High chemical resistance and temperature
tolerance/

Available natural or black/

Item No. 781201-906 for Acoustic Liquid Handling

nonpyrogenic

Well format: 384, Bottom: solid, Raw material: PP, Surface treatment: untreated, Lid: no

Item no.

132 /Well profile

Product colour

781201

F-bottoml natural

784201

V-bottoml natural

781201-906

F-bottoml natural

www.gbo.com

Plate geometry

Deep Well

Plate design

Small Volume

Working volume (well)

Qty. inner / outer

15 µl - 145 µl

10 / 100

1 µl - 90 µl

10 / 10015 µl - 145 µl

10 / 100

HTS-Microplates
384 Well Microplates

Item no.

Well profile

Product colour

781209

F-bottom

781280V-bottomPlate geometry

Plate design

Working volume (well)

Qty. inner / outerl black

15 µl - 145 µl

10 / 100l natural

13 µl - 120 µl

10 / 100

www.gbo.com

/ 133

HTS-Microplates
1536 Well Microplates/

For cell culture treatedmicroplates, pleaserefer to chapter cell
culture/Available inpolystyrene,
polypropylene andcycloolefin/

Clear / black / white/

HiBase for top-reading
applications/

Solid bottom or µClear ®
film bottom/

Sterile / non-sterile/

Non-treated or inhigh-binding quality

1536 WELL MICROPLATESThe highest possible degreeof automation, optimal performance and cost savings
continue to be the requirements placed on microplates
for high-throughput screening.
In 1997, shortly after the launchof the 384 well microplates,
Greiner Bio-One was the firstmanufacturer to introduce another innovative microplate format – the 1536 well microplate.
The external dimensions were134 /www.gbo.com

the same as those used in the
96 well and 384 well microplates. However, to utilise the available space most efficiently, thenumber of wells was increased
fourfold from 384 to 1536.
Close cooperation with numerous users has now led to thedevelopment of a broad productrange, and the constant drive towards improvements in quality
has, for example, led to a reduction in curvature of the plates to< 100 µm.

HTS-Microplates
1536 Well Microplates

1536 Well Microplates

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Solid bottom or µClear ® film bottom/

Sterile / non-sterile/

Non-treated or in high-binding quality

nonpyrogenic

Well format: 1536, Well profile: F-bottom, Raw material: PS, Plate geometry: HiBase,
Working volume (well): 3 µl - 10 µl, Lid: no

Item no.

Bottom782101solid

782061solid

782075solid

782074solid

782076solid

782077solid

782095µClear®

782097µClear®

782096µClear®

Binding characterisitc

Binding brand nameSurface treatment

untreated
high-binding

MICROLON 600
LUMITRAC 600

high-binding

FLUOTRAC 600
untreated

high-binding

l black

15 / 60
15 / 60+

15 / 60
15 / 60+l whitel black

untreated+l blackl black

Qty. inner / outer

15 / 60l whitel whiteuntreated

Sterilel clearl clearuntreated
high-binding

Product colour

15 / 60
15 / 60+

15 / 60
15 / 60

www.gbo.com

/ 135

HTS-Microplates
Polypropylene Storage Plates/Compound storage
plates for acoustic
liquid handling canalso be found in thischapter.

/

Ideally suited for the
storage of active
agents, DNA/RNA orstock cultures/

High chemical
resistance andtemperature tolerance

POLYPROPYLENE STORAGE PLATESGreiner Bio-One polypropylene microplates are perfect storage plates for active agents, patient samples or
biomolecules.
Their most important properties are biological inertness, resistance to numerous solventscommonly used in the laboratory, such as DMSO and hightemperature resistance. The

136 /www.gbo.com

footprint is compatible with
automated systems.
The microplates are also characterised by elevated well walls
which make it possible to easily
seal them. The portfolio ranges
from the 96 well MASTERBLOCK®
with volumes of 0.5 ml, 1 ml or
2 ml over a MASTERBLOCK® with
384 wells or 1536 wells up to the
96 well storage box.

HTS-Microplates
Polypropylene Storage Plates

96 Well MASTERBLOCK®
Polypropylene

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Ideal for storing non-human sample material andcultivating bacteria / yeast/

Uniform external dimensions and tolerances/

Alphanumeric well coding/

Sealable with adhesive films and heat sealers/

Sealable with CapMats

nonpyrogenic

Well format: 96, Bottom: solid, Raw material: PP
Item no.

Well profile

Product colour

Total volume (well)

Lid780201

U-bottom

780261

SterileQty. inner / outerl natural

1 ml

CapMat 381070, 381061

U-bottoml natural

1 ml

CapMat 381070, 381061

780215

U-bottoml natural

1 ml

CapMat 381070, 381061

5 / 50786201

V-bottoml natural

0.5 mlCapMat 381070, 381061

8 / 80786261

V-bottoml natural

0.5 mlCapMat 381070, 381061

780270V-bottoml natural

2 ml

CapMat 381080, 381081

780271

V-bottoml natural

2 ml

CapMat 381080, 381081

780285

V-bottoml natural

2 ml

CapMat 381080, 381081

1 / 50+1 / 50+1 / 801 / 50+1 / 505 / 5096 Well Storage Box

Item no.

/Storage box made of polycarbonate/

With / without 96 polypropylene vessels/

Coding card included/

All components autoclavable

Qty. inner / outer

975502

1 / 1209755701 / 50www.gbo.com

/ 137

HTS-Microplates
Polypropylene Storage Plates

384 Deep Well MASTERBLOCK®
Polypropylene

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Ideal for compound libraries and applications withlarger volumes/

Conical well shape for precise pipetting/

Alphanumeric well coding/

Sealable with adhesive films and heat sealers

nonpyrogenic

Well format: 384, Well profile: V-bottom, Bottom: solid, Raw material: PP, Plate geometry: Deep Well, Lid: no

Item no.

Product colour

781270l natural

781271l natural

SterileQty. inner / outer6 / 60+6 / 601536 Deep Well Microplates
Polypropylene

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Uniform external dimensions and tolerances/

Total volume of 18 µl/

Alphanumeric well coding/

Sealable with adhesive films and heat sealers

nonpyrogenic

Well format: 1536, Well profile: V-bottom, Bottom: solid, Raw material: PP, Plate geometry: Deep Well,
Working volume: 3 µl - 15 µl, Lid: no

Item no.

138 /Product colour

782261l natural

782270l natural

www.gbo.com

SterileQty. inner / outer+

15 / 60
15 / 60

HTS-Microplates
Microplates for Compound Storage/

You can find more
information about thisproduct in our webshop.

MICROPLATES FOR COMPOUND STORAGE/

Suited for compoundstorage/

Resistant against polar
solvents/

Excellent water andvapour barrier function/

Almost no leachables/

Low biomoleculebinding/

Glass-like opticalproperties

Polypropylene is still the material of choice for storage plates,
but the material class of cycloolefins is becoming more routinely used because of its unsurpassed performance for a wide
range of applications.
In compound storage, plates
made from cycloolefins offer thebest combination of chemical
resistance to polar solvents, like
DMSO, and optical clarity. In addition, the dimensional stability
and glass-like optical properties
make this material ideally suited for plates in fully automatedsystems.

www.gbo.com

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HTS-Microplates
Microplates for Compound Storage

Microplates for Compound Storage
384 and 1536 Well
Microplates for Acoustic Liquid Handling/

Stringent production specifications for a constantbottom quality/

Microplates are deionised and packed in antistatic
bags

Well profile: F-bottom, Bottom: solid, Lid: no

140 /

Plate
geometry

Item no.

Well format

Raw material

Surface treatment

Product colour

781201-906

384

PP

untreatedl natural

793855384

cycloolefin

l clear

HiBase7828551536

cycloolefin

l clear

HiBase792870-906

1536

cycloolefin

l clearwww.gbo.com

Plate design

Small
VolumeWorking volume(well)

Qty. inner /outer

15 µl - 145 µl

10 / 100

1 µl - 25 µl

15 / 60

1 µl - 10 µl

15 / 60

1 µl - 14 µl

15 / 60

HTS-Microplates
Non-binding Microplates/

For more detailed
information on well
profiles and technicaldetails please refer toour data sheets on our
website: www.gbo.com

NON-BINDING MICROPLATES/

96 / 384 / 1536 wellformat/Clear / black / white/

Solid bottom or µClear ®
film bottom/

Ultra low non-specificbiomolecular binding
properties/

Long-term surface
stability without
degradation or leaching

Polystyrene microplates with
non-treated surfaces are commonly used for homogeneous
biochemical HTS assays anddemonstrate low and reproducible biomolecule binding.
However, even low amounts of
biomolecular binding (e. g. DNA,
RNA, proteins, peptides) cancause an undesirable increasein background, resulting in decreased signal-to-noise ratio.

Greiner Bio-One’s non-binding
microplate surfaces prevent unwanted non-specific binding,especially advantageous forsensitive biochemical assays.
Characterised by low protein, DNA, RNA and peptide binding properties the non-binding
surfaces significantly increase
assay sensitivity by reducing
background and improving signal-to-noise ratio.

www.gbo.com

/ 141

HTS-Microplates
Non-binding Microplates

Non-binding Microplates
96 Well

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Clear / black / white/

U-bottom, V-bottom or F-bottom/chimney well/

Solid bottom or µClear ® film bottom

nonpyrogenic

Well format: 96, Raw material: PS, Binding characterisitc: Non-binding, Lid: no

Item no.

Well profile

BottomProduct colour

Working volume (well)

Qty. inner / outer

650901

U-bottomsolid

l clear

40 µl - 280 µl

10 / 40651901

V-bottomsolid

l clear

40 µl - 200 µl

10 / 40655901

F-bottom / Chimney Wellsolid

l clear

25 µl - 340 µl

10 / 40655904

F-bottom / Chimney Wellsolidl white

25 µl - 340 µl

10 / 40655900F-bottom / Chimney Wellsolidl black

25 µl - 340 µl

10 / 40655903F-bottom / Chimney Well

µClear®

l white

25 µl - 340 µl

10 / 40655906

F-bottom / Chimney Well

µClear®

l black

25 µl - 340 µl

10 / 40

Non-binding Microplates
384 and 1536 Well/

Clear / black / white/With F-bottom or as Small Volume option/

Solid bottom or µClear ® film bottom

Well profile: F-bottom, Raw material: PS, Binding characterisitc: Non-binding, Lid: no

Item no.

142 /Well format

BottomProduct colour

781901384

solid

781904384

781900Working volume (well)

Qty. inner / outerl clear

15 µl - 110 µl

10 / 40solid

l white15 µl - 110 µl

10 / 40

384

solid

l black

15 µl - 110 µl

10 / 40781903384µClear®

l white15 µl - 110 µl

10 / 40781906384µClear®

l black

15 µl - 110 µl

10 / 40

www.gbo.com

Plate geometry

Plate design

HTS-Microplates
Non-binding Microplates

Item no.

Well format

BottomProduct colour

Plate geometry

Plate design

Working volume (well)

Qty. inner / outer

784904384

solid

l whiteHiBaseSmall Volume

4 µl - 25 µl

10 / 40784900384

solid

l black

HiBaseSmall Volume

4 µl - 25 µl

10 / 40782904

1536

solid

l whiteHiBase3 µl - 10 µl

15 / 60

782900

1536

solid

l black

HiBase3 µl - 10 µl

15 / 60

www.gbo.com

/ 143

HTS-Microplates
Streptavidin-coated Microplates/

For more detailed
information on well
profiles and technicaldetails please refer toour data sheets on our
website: www.gbo.com/

Available in 96 well and
384 well format/

Clear / black / white/

3-year shelf life at room
temperature/

All plates pre-blocked
and ready-to-use

STREPTAVIDIN-COATEDMICROPLATESStreptavidin-coated solid phases serve as reliable binding
surfaces for all types of biotinylated molecules.
Numerous ligands can be biotinylated simply and due to thelow molecular weight of biotin(244 Da) the functionality of themolecules is normally not impaired. Thus streptavidin-coatedsolid phases make it possible torapidly isolate, determine andquantify components from a reaction mixture. By immobilising

144 /www.gbo.com

the biotinylated substance, it isalso possible to reproduce complete reaction chains on a streptavidin solid phase, e. g. enzyme
immunoassays, enzyme activity
assays, DNA hybridisation techniques and quantification of PCR
products.
The high-purity streptavidin isbound to the plate surface in a
uniform and stable layer. The coefficient of variation from wellto well is under 5 % for 96 well
microplates and under 8 % for
384 well microplates.

HTS-Microplates
Streptavidin-coated Microplates

Streptavidin-coatedMicroplates 96 and 384 Well/

Long shelf life at room temperature/

Lot number on each pack/Pre-blocked with BSA

Bottom: solid, Raw material: PS, Surface treatment: Streptavidin, Lid: no

Item no.

Well format

Well profile

Product colour

Qty. inner / outer

65599096

C-bottoml clear5 / 4065599496

F-bottoml white5 / 4065599796

C-bottoml black

5 / 40781990384

F-bottoml clear5 / 40781995384

F-bottoml white5 / 40781997384

F-bottoml black

5 / 40/ Further streptavidin-coated microplates are available on request.

www.gbo.com

/ 145

HTS-Microplates
UV-Star® Microplates/

Application Note: UV/VIS Spectroscopy in
Microplates UV-Star®,µClear®, MICROLON andCELLSTAR®

/

Available in 96 well and
384 well format/

With cycloolefin film
bottom

/Optical window down to
230 nm ideal for nucleic
acid determinations at
260 nm/280 nm/

For measurements ofprotein concentration
at 280 nm

UV-STAR® MICROPLATESUV / VIS spectroscopy is aclassical analytical methodfor determining the chemical constitution of a substance and its concentration inaqueous solution.
UV / VIS spectroscopy is usually conducted in quartz glass cuvettes. However, cuvettes do notprovide sufficient throughput
when dealing with large amountsof samples, and microplates canbe used to speed up work.
Standard polystyrene microplates are only partially suitable for transmission measurements in the UV. Polystyrene
absorbs UV especially in the
short-wavelength range (< 320nm). µClear ® microplates with athin polystyrene film base alrea-

146 /www.gbo.com

dy have much lower backgroundvalues and can be used up to 340
nm without any problem. The adaptation of the patented µClear ®
process technology to a new, innovative UV-transparent material has made it possible to produce microplates that extend
the transmission range up to
230 nm.
For the determination of nucleic acid and protein concentrations at 260 nm or 280 nm without background interference
UV-Star ® microplates are theideal alternative to expensive
and fragile quartz glass plates
or cuvettes. UV-Star ® plates arealso DMSO-resistant and canbe stored at -20 °C without any
problem.

HTS-Microplates
UV-Star® Microplates

UV-Star® Microplates

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Available in 96 well and 384 well format/

With cycloolefin film bottom/

Optical window down to 230 nm ideal for nucleic
acid determinations at 260 nm/280 nm/

For measurements of protein concentration at
280 nmnonpyrogenic

Bottom: Cycloolefin film, Raw material: COC, Surface treatment: untreated, Lid: no

Item no.

Well format

Well profile

Product colour

655801

96

F-bottom / ChimneyWelll clear675801

96

F-bottoml clear

781801384

F-bottoml clear788876384

F-bottoml black

Plate geometry

Plate design

half area

LoBaseSmall
VolumeWorking volume(well)

Qty. inner /outer

25 µl - 340 µl

10 / 40

15 µl - 175 µl

10 / 40

15 µl - 110 µl

10 / 40

4 µl - 25 µl

10 / 80

www.gbo.com

/ 147

HTS-Microplates
Lids / Sealers / CapMats

LIDS / SEALERS / CAPMATS
Lids offer protection against
contamination and evaporation during sample storage andcell cultivation.
In addition, Greiner Bio-One offers a full set of adhesive sealers
for a wide range of routine andspecialised applications, e.g.
high-throughput screening, im-148 /www.gbo.com

munology, microbiology, molecular biology and PCR.
An alternative method for sealing 96 well plates are CapMats.
They are made of ethyl vinyl
acetate (EVA), are resistant to
DMSO and can be used in a temperature range between -20 °C
and +60 °C. CapMats are notpierceable.

/

Forum No 6: Sealers for
microplates and theirareas of application inmolecular biology andcell culture (F073013)/

Polystyrene lids inthree different
profile heights with andwithout condensation
rings

/

Classical adhesive
films coated withacrylate adhesive as
well as advanced
adhesive films withpressure-sensitive
silicone adhesive/

EVA CapMats forsealing of 96 wellplatesHTS-Microplates
Lids / Sealers / CapMats

Lids
All sterile lids are non-cytotoxic.

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

nonpyrogenic

Description: Lid, Raw material: PS
Item no.

HeightProduct colour

Condensation rings

Lid type

6561019 mm

l clearnohigh6561619 mm

l clearnohigh6561709 mm

l clearyeshigh6561719 mm

l clearyeshigh6561906 mm

l clearnoflat656191

6 mm

l clearnoflat691101

4.8 mml clearnoultra low

691161

4.8 mml clearnoultra low

SterileQty. inner / outer1 / 100+

1 / 100
1 / 100+

1 / 100
20 / 200+

20 / 200
25 / 100+

25 / 100

Sealers
The classical sealers such as EASYseal,
AMPLIseal, SILVERseal and BREATHseal
are coated with an acrylate adhesive. The
advanced sealer VIEWseal is coated with apressure-sensitive silicone adhesive.

Item no.

Description

FeaturePierceable

SterileQty. inner / outer

676001EASYsealclear

676090SILVERseal

aluminium foil

676070VIEWsealclear

100 / 1.200

676040AMPLIsealclear

100 / 2.000

676050BREATHseal

Gas-permeable

50 / 2.500

100 / 2.000
yes

100 / 1.200

www.gbo.com

/ 149

HTS-Microplates
Lids / Sealers / CapMats

Item no.

676051

Description

FeaturePierceable

BREATHseal

Gas-permeable

SterileQty. inner / outer+

50 / 500/ Forum No. 6: Sealers for microplates and their areas of application in molecular biology and cell culture (F073013)

CapMats

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Made of ethyl vinyl acetate (EVA)/

Available for 96 well microplates and
MASTERBLOCK®

/Sterile / non-sterile

nonpyrogenic

Description: 96 Well CapMat, Pierceable: no, Raw material: EVA
Item no.

150 /Nap shape

381070round

381061round

381080square381081squarewww.gbo.com

SterileQty. inner / outer

10 / 50+

1 / 5010 / 50+

1 / 50HTS-Microplates
Protein Crystallisation Plates/

Forum No. 7: Advanced
high-throughput
platforms forprotein crystallisation(F073016)

PROTEIN CRYSTALLISATION PLATES/For vapour diffusion or
microbatch under oil/

Made of polystyrene orpolyolefin (LBR plates)/

Footprint conforming
to ANSI 1-2004 (except
Terasaki plates)/

Barcode labelling onrequest/

CrystalQuick plateswith alphanumeric wellcoding

Detailed knowledge of interactions between the structureand function of biomolecules
is fundamental for biological,
medical and pharmaceuticalresearch. A reliable method is
the X-ray structure analysis ofprotein crystals.
In order to show three-dimensional molecular structures, theproteins must first be crystallised. Since every protein is different and a variety of factors
influence protein crystallisation,
the optimal conditions are usually tested with high-throughput
technologies (pipetting robots).

Greiner Bio-One has speciallydeveloped microplates and accessories of the CrystalStar series to meet the requirements ofhigh-throughput crystallisation
in a short time and with relatively
small amounts of protein. Techniques such as vapour diffusion(sitting drop, hanging drop) andmicrobatch under oil can be carried out effectively.
Plates with hydrophobic surface are particularly well suitedfor nanolitre crystallisation of
membrane proteins. LBR (lowbirefringence) plates are specifically designed for the use ofpolarised light.

www.gbo.com

/ 151

HTS-Microplates
Protein Crystallisation Plates

96 Well CrystalQuick /CrystalQuick Plus Plates/

Crystallisation plates for sitting drop applications
with different well profiles and material properties/

Optimised for sealing with VIEWseal or AMPLIseal/

Alphanumeric well coding

Well format: 96
HeightMaterial properties

Well profile

Plate design

Well /
reservoir

Well volume,max

Volume perreservoir

Qty. inner /outer6091718 mmstandard

flat

square (LP)1

3.9 µl140 µl20 / 80

6091808 mm

hydrophobicflatsquare (LP)1

3.9 µl140 µl20 / 80609871

8 mm

LBR

flat

square (LP)1

3.9 µl140 µl20 / 80

60910114.4 mmstandard

flat

square (SW)34.1 µl

320 µl10 / 4060912014.4 mmstandard

concaveround (RW)3

1.9 µl

320 µl10 / 40

60913014.4 mmhydrophobicflatsquare (SW)34.1 µl

320 µl10 / 4060980114.4 mm

LBR

flat

square (SW)34.1 µl

320 µl10 / 4060982014.4 mm

LBR

concaveround (RW)3

1.9 µl

320 µl10 / 4060983014.4 mm

LBR, hydrophobicflatsquare (SW)34.1 µl

320 µl10 / 40

Item no.

24 Well ComboPlate
CrystalBridge / Coverslips

Item no.

152 /Description

Feature/Universal 24 well crystallisation plate with coverslips and CrystalBridge for sitting drop applications/

Siliconised coverslips available

Well format

Ø wellBottom shape

Well volume,
max

Lid

Qty. inner /outer662150ComboPlate24

16.3 mmflat

3,300 µlyes

6 / 24662145

CrystalBridge

1

4.6 mmconcave45 µl

no- /250501870round coverslips siliconised glass
18 mm
0.19 - 0,22 mm

100 / 1.000

503870round coverslips siliconised glass
22 mm
0.19 - 0,22 mm

100 / 1.000

www.gbo.com

HTS-Microplates
Protein Crystallisation Plates

Item no.

503850Description

FeatureWell format

Ø wellround coverslips siliconised glass
22 mm
0.5 - 0,6 mm

Bottom shape

Well volume,
max

Lid

Qty. inner /outer

100 / 1.000/ Siliconised coverslips for Linbro plates (round, ø 22 mm) are available on request.

www.gbo.com

/ 153

The ELISA technique (Enzyme-Linked Immunosorbent Assay) is
one of the most widely used biochemical methods in analyticallaboratories and in diagnostics. With this method, analytes suchas peptides, proteins, antibodies and hormones are detected andquantified selectively and in low concentrations. In addition, ELISAs
can be automated and carried out with a high sample throughput,are relatively inexpensive and the test results are available
promptly.

154 /www.gbo.com

Immunology / HLA﻿

IMMUNOLOGY / HLA/ ELISA Microplates ������������������������������160
96 Well ELISA Microplates U-Bottom /V-Bottom / F-Bottom / Half Area�������������������� 161/ ELISA Strip Plates ������������������������������ 162
96 Well ELISA Strip Plates ����������������������������� 163
Single-break Strip Plates ������������������������������ 164/ Immuno Tubes ������������������������������������ 165
Immuno Tubes ���������������������������������������������� 166/ Terasaki Plates ����������������������������������� 167
Terasaki Plates ��������������������������������������������� 168

www.gbo.com

/ 155

IMMUNOLOGY/ High-binding and medium-binding surface properties/ Strips mounted in frame/ Strips with 8 or 16 wells (U-bottom / F-bottom)/ Clear / black / white microplates/ Alphanumeric coding

156 /www.gbo.com

FOR DIAGNOSTICS ANDIMMUNOLOGICAL RESEARCHELISA (Enzyme-Linked Immunosorbent Assay) is probably the most widely used biochemical method in laboratory
analysis and diagnostics.
Analytes such as peptides, proteins, antibodies and hormones
can be detected selectively in
low concentrations among a
multitude of other substancesand be quantified. Additionally, ELISAs are rapid, sensitive,
cost-effective and can be performed in a high-throughput
manner.
ELISA is used in a variety of different assay types (e.g. direct
ELISA, indirect ELISA, sandwich
ELISA, competitive ELISA). Nevertheless, all ELISA variants are
based on the same principle: thebinding of one assay component– antigen or specific antibody –to a solid surface and the selective interaction between bothassay components.
Molecules not specifically interacting with the assay component bound to the solid surface are washed away during theassay. For detection of the interaction the antibody or antigen islabelled or linked to an enzyme(direct ELISA). Alternatively, asecondary antibody conjugate
can be used (indirect ELISA). The
assay is processed by adding an
enzymatic substrate to produce a measurable signal (colorimetric, fluorescent or luminescent). The strength of the signal
indicates the quantity of analytes in the sample.

LITERATURE:/

Forum No. 9:
Microplates for
enzyme-linked
immunosorbent
assays (ELISA)(F073004)/

Application Note
„Insulin ELISA
on high-binding
MICROLON 600 and
CELLSTAR ® microplates“ (F073106)/

Application Note
„Influence of
coating buffer
and incubation
conditions on ELISA
performance “(F073118)

Greiner Bio-One has
been manufacturing
microplates for diagnostics and immunological research forover 30 years.

www.gbo.com

/ 157

SURFACE PROPERTIES AND MICROPLATE COLOUR
A key step in ELISA is the binding of one
assay component – antigen or antibody –to the solid surface by passive adsorption.
Therefore, the features of this surface
are crucial for the performance of theassay. All ELISA microplates from Greiner
Bio-One are made out of high-quality virgin polystyrene. The resin is highly transparent and therefore ideally suited for optical measurements. Due to its chemical
nature polystyrene is a hydrophobic compound. Hydrophilic groups can be introduced to polystyrene surfaces by physical treatment. Greiner Bio-One offers two
surface qualities for ELISA microplates:
the hydrophilic high-binding products
and the less hydrophilic medium-binding

158 /www.gbo.com

products.
Since attachment to a solid surface based
upon passive adsorption depends as wellon the properties of the molecule to bebound, it is therefore advisable to compare the performance of high-binding andmedium-binding products when developing a new assay.
Beside products made of clear polystyrene
for colorimetric measurements, Greiner
Bio-One offers a wide variety of black andwhite ELISA microplates for luminescenceand fluorescence measurements.

The main criterion for
our ELISA microplates
is a stable coefficientof variation (CV) frombatch to batch which
is monitored over along period.

QUALITY ASPECTS
We set high standards on the quality of
our immunological products, especiallyon consistency and reproducibility of binding properties. As the raw material has
a major influence on the binding proper-

ties of the final product, the incoming raw
material used for ELISA microplates isroutinely monitored for identity andimmunological quality.

MICROLONFLUOTRACLUMITRAC

Transparent microplateswith medium-binding(MICROLON 200)
or high-binding(MICROLON 600) surface

Black microplateswith medium-binding(FLUOTRAC 200)
or high-binding(FLUOTRAC 600) surface

White microplateswith medium-binding(LUMITRAC 200)
or high-binding(LUMITRAC 600) surfacewww.gbo.com

/ 159

Immunology / HLA
ELISA Microplates/

You can find more
information about thisproduct in our
webshop.

/

With U-Bottom /V-Bottom / F-Bottom
standard (ST) / F-bottom chimney well /halfarea well geometry/

With medium and highbinding surfaces/

Made of clear / black /white polystyrene fortransmission, fluorescence orluminescencemeasurements

ELISA MICROPLATESGreiner Bio-One has been manufacturing microplates fordiagnostics and immunological research for over 30
years.
The microplate footprint is compatible with automated systems.
A key step in ELISA is the binding of one assay component –antigen or antibody – to the solid
surface by passive adsorption.
Therefore, the features of thissurface are crucial for the
performance of the assay. All
ELISA microplates from Greiner

160 /www.gbo.com

Bio-One are made out of highquality virgin polystyrene. The
resin is highly transparent andtherefore ideally suited for optical measurements.
Beside products made of clear
polystyrene for colorimetricmeasurements, Greiner Bio-One
offers a wide variety of black andwhite ELISA microplates for luminescence and fluorescencemeasurements.
The quality of our immunologicalproducts is constantly controlled in our quality assurance laboratory by means of ELISA.

Immunology / HLA
ELISA Microplates

96 Well ELISA Microplates
U-Bottom / V-Bottom / F-Bottom / Half
Area/

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

Manufactured from crystal clear polystyrene

nonpyrogenic

Well format: 96, Bottom: solid, Raw material: PS, Lid: no
Well profile

Bindingcharacterisitc

Binding brand nameProduct colour

Working volume(well)

Qty. inner /outer650001

U-bottommed.-binding

MICROLON 200

l clear

40 µl - 280 µl

10 / 40

650061

U-bottomhigh-binding

MICROLON 600

l clear

40 µl - 280 µl

10 / 40

650061

U-bottomhigh-binding

MICROLON 600

l clear

40 µl - 280 µl

10 / 40

651001

V-bottommed.-binding

MICROLON 200

l clear

40 µl - 200 µl

10 / 40651061

V-bottomhigh-binding

MICROLON 600

l clear

40 µl - 200 µl

10 / 40655001

F-bottom/ST

med.-binding

MICROLON 200

l clear

25 µl - 340 µl

10 / 40655061

F-bottom/ST

high-binding

MICROLON 600

l clear

25 µl - 340 µl

10 / 40655080F-bottom /Chimney Well

med.-binding

MICROLON 200

l clear

25 µl - 340 µl

10 / 40655081

F-bottom /Chimney Well

high-binding

MICROLON 600

l clear

25 µl - 340 µl

10 / 40675001

F-bottommed.-binding

MICROLON 200

l clearhalf area

15 µl - 175 µl

10 / 40675061

F-bottomhigh-binding

MICROLON 600

l clearhalf area

15 µl - 175 µl

10 / 40

Item no.

Plate designwww.gbo.com

/ 161

Immunology / HLA
ELISA Strip Plates/

ELISA STRIP PLATESGreiner Bio-One is offering alarge variety of 96 well strip
microplates for diagnostic
and immunological research
applications.
Microplates in strip format offer
the advantage of greater flexibility in diagnostics. Individual
strips can be removed from the
support frame so that the number of tests to be performed
can be adjusted to the numberof samples and is not predetermined by the microplate format
used. In addition, the individual
strips can be subjected to a wide
variety of different test conditions. The quality of our immunological products is constantly

162 /www.gbo.com

controlled in our quality assurance laboratory by means of ELISA.
Clear MICROLON products aretested in a colorimetric immunoassay, black FLUOTRAC plates in a fluorescence immunoassay, white LUMITRAC plates ina luminescence immunoassay.
The portfolio is completed by
C8 single-break strip plates for
immunological applications.
These are supplied as twelve
8-well strips in a support framewith 96 spaces (12 x 8 matrix).
The individual wells can be
broken off separately (“singlebreak” option) and the numberof tests performed can thus be
precisely adjusted to the number of samples.

You can find more
information about thisproduct in our
webshop.

Immunology / HLA
ELISA Strip Plates

96 Well ELISA Strip Plates

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Strips mounted in frame/

Black and white F16 and U16 strip plates on request

nonpyrogenic

Well format: 96, Bottom: solid, Raw material: PS, Lid: no
Well profile

Bindingcharacterisitc

Binding brand nameProduct colour

Plate design

Working volume(well)

Qty. inner /outer767070

U-bottommed.-binding

MICROLON 200

l clearstrip plate
12x8

50 µl - 280 µl

10 / 100

767071

U-bottomhigh-binding

MICROLON 600

l clearstrip plate
12x8

50 µl - 280 µl

10 / 100

762070F-bottommed.-binding

MICROLON 200

l clearstrip plate
12x8

20 µl - 350 µl

10 / 100

762071

F-bottomhigh-binding

MICROLON 600

l clearstrip plate
12x8

20 µl - 350 µl

10 / 100

762075F-bottommed.-binding

LUMITRAC 200

l whitestrip plate
12x8

20 µl - 350 µl

10 / 100

762074

F-bottomhigh-binding

LUMITRAC 600

l whitestrip plate
12x8

20 µl - 350 µl

10 / 100

762076

F-bottommed.-binding

FLUOTRAC 200

l black

strip plate
12x8

20 µl - 350 µl

10 / 100

762077

F-bottomhigh-binding

FLUOTRAC 600

l black

strip plate
12x8

20 µl - 350 µl

10 / 100

756070F-bottommed.-binding

MICROLON 200

l clearstrip plate
6x18

20 µl - 350 µl

10 / 100

756071

F-bottomhigh-binding

MICROLON 600

l clearstrip plate
6x18

20 µl - 350 µl

10 / 100

754070

U-bottommed.-binding

MICROLON 200

l clearstrip plate
6x18

50 µl - 280 µl

10 / 100

754061

U-bottomhigh-binding

MICROLON 600

l clearstrip plate
6x18

50 µl - 280 µl

10 / 100Item no.

www.gbo.com

/ 163

Immunology / HLA
ELISA Strip Plates

Single-break Strip Plates

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Clear with / without colour coding/

Strips mounted in frame/

C8 Single-break strip plates without colour coding
cell culture treated on request

nonpyrogenic

Well format: 96, Well profile: C-bottom, Bottom: solid, Raw material: PS, Plate design: strip plate 12x8,
Working volume (well): 20 µl - 300 µl, Lid: no

Item no.

164 /Binding characterisitc

Binding brand nameColour stripe

705070med.-binding

MICROLON 200

l clear10 / 100

705071high-binding

MICROLON 600

l clear10 / 100

705063

med.-binding

MICROLON 200

l clearl red

10 / 100

705073high-binding

MICROLON 600

l clearl red

10 / 100

705074high-binding

MICROLON 600

l clearl blue10 / 100

705065

med.-binding

MICROLON 200

l clearl green

10 / 100

705075high-binding

MICROLON 600

l clearl green

10 / 100

705066

med.-binding

MICROLON 200

l clearl yellow

10 / 100

705076high-binding

MICROLON 600

l clearl yellow

10 / 100

www.gbo.com

Colour coding well rim

Qty. inner / outerImmunology / HLA
Immuno Tubes/

More tubes can befound in the chapter
Tubes / Multipurpose
containers

IMMUNO TUBES
Immuno tubes are often used for
determining hormone levels, forexample TSH (thyroid stimulating hormone). With a length of
75 mm and a diameter of 12 mmwith and without a “star”, they areavailable in both MICROLON 200
and MICROLON 600 quality. The
so-called “star” at the bottom of

the tubes serves to increase thesurface and thus makes it possible to bind larger amounts ofantigens or antibodies.
The quality of our immunologicalproducts is constantly controlled in our quality assurance laboratory by means of ELISA.

www.gbo.com

/ 165

Immunology / HLA
Immuno Tubes

Immuno Tubes/

More tubes can be found in the chapter Tubes /Multipurpose containers

Height: 75 mm, Ø: 12 mm, Raw material: PS, Nominal volume: 5 ml

Item no.

166 /Binding characterisitc

Binding brand name

Bottom shape

Qty. inner / outer

115001

med.-binding

MICROLON 200

round

250 / 2.000

115061high-binding

MICROLON 600

round

250 / 2.000

115070med.-binding

MICROLON 200

round with star

250 / 2.000

115071high-binding

MICROLON 600

round with star

250 / 2.000

www.gbo.com

Immunology / HLATerasaki Plates/

We provide Terasaki
plates with one years‘
stability on the surface
treatment.

TERASAKI PLATESGreiner Bio-One Terasaki plates are suitable for all applications for serological determination of HLA antigens.
The plates are supplied eitherwith 60 or 72 wells, the plate
dimensions stay the same.
The human leucocyte antigen(HLA) system is the major histocompatibility complex (MHC) of
humans and is composed of thetwo polymorphic classes HLA-I(A, B, and C) and HLA-II (DR, DQ,
and DP). Basically, four different
areas of indication can be distinguished for HLA typing: transplantation, transfusion, diseaseassociation and forensic.
The serological determinationof HLA proteins of the HLA-A,-B, -C and -DR genetic positions
is primarily performed with thecomplement-dependent microlymphocytotoxicity test (LCT) or
Terasaki test, which has beenstandardised since 1964. The
basis for this test method is thecytolysis of the lymphocytes tobe tested, which is caused by the
antibody-antigen mediated activation of the complement system. Permeabilised lymphocytes are generally stained withchromophores or fluorophores
and evaluated microscopically.

www.gbo.com

/ 167

Immunology / HLATerasaki Plates

Terasaki Plates/

60 well and 72 well Terasaki plates/

Manufactured from crystal clear polystyrene/

Item 659180 contains one lid per bag

noncytotoxic

Surface treatment: TC, Well volume, max: 11.5 µl, Working volume (well): ≤10 µl, Lid: yes

Item no.

168 /Well format

Stackable

Qty. inner / outer

65318060

no10 / 270

65319060

no120 / 480

65918060

yes

10 / 200

65919060

yes

150 / 1.200

65418072

no10 / 270

76919072

yes

150 / 1.200

www.gbo.com

Immunology / HLATerasaki Plates

www.gbo.com

/ 169

The study of microorganisms, their metabolism and their effects
on other (macro-) organisms generally refers to the field of
microbiology. The various detection methods are used in many
research areas and in industry. For example, tests for the detectionof microorganisms and for quality assurance are also applied in the
production of pharmaceuticals, cosmetics, food and beverages.

170 /www.gbo.com

Microbiology
﻿

MICROBIOLOGY/ Dishes / Plates / Others ���������������������� 172
Petri Dishes �������������������������������������������������� 173
Contact Dishes Divided Petri Dish������������������ 173
Macroplate / Square Petri Dish /OneWell Plate�������������������������������������������������174
Swab Tube Cotton Swab�������������������������������� 175
Disposable Inoculation Loops / Needles ������� 175
Disposal Bags ������������������������������������������������176

www.gbo.com

/ 171

Microbiology
Dishes / Plates / Others/

DISHES / PLATES / OTHERS
Greiner Bio-One offers petri dishes with diameters of 35, 60, 94,
100 and 145 mm. Manufactured
from highly transparent polystyrene (PS), they are heat-resistant up to 60 °C for use with
hot agar. Depending on the application vented and non-vented design can be selected. Besides the standard round design,
a square-profile dish (120 mm x
120 mm x 17 mm) and the OneWell Plate in SBS format areavailable.

172 /www.gbo.com

Furthermore, Greiner Bio-One
offers contact dishes which areused with hygiene monitoring including detection of contaminations as well as testing theeffectiveness of cleaning anddisinfection on plane surfaces
by means of contact cultures.
The product range is completed by a special model with twocompartments which enables
parallel experiments with different samples or parameters
in one dish.

For exact dimensionsof our petri dishes,
please refer to the
product data sheets on
our website.

Microbiology
Dishes / Plates / Others

Petri Dishes/

Available in different sizes/

Easy stacking/

With or without vents/

Manufactured from crystal clear polystyrene

noncytotoxic

Item no.

HeightØ nominal size

Vent nock

SterileQty. inner / outer

627102

10 mm

35 mm

yes

63916120 mm

145 mmyes

628102

15 mm

60 mm

yes

628161

15 mm

60 mm

yes

632180

16 mm

94 mm

no

20 / 480

633180

16 mm

94 mm

yes

20 / 480

633181

16 mm

94 mm

yes

66410220 mm

100 mmyes

664161

20 mm

100 mmyes

63910220 mm

145 mmyes

10 / 740+

15 / 120
20 / 600+

20 / 600+

20 / 480
15 / 360+

15 / 360
15 / 120

Contact Dishes
Divided Petri Dish/

With or without vents/

Manufactured from crystal clear polystyrene/

Contact dish (sterile) with graduation to enable
quick and easy analyses

noncytotoxic

Height: 15 mm
Item no.

629161Featuregraduated

CompartmentsØ nominal size

65 mm

Total volume (well)

Vent nock

SterileQty. inner / outerno+20 / 600

www.gbo.com

/ 173

Microbiology
Dishes / Plates / Others

Item no.

FeatureCompartments

629180

graduated

635102

2 compartmentsØ nominal size

Total volume (well)

Vent nock

SterileQty. inner / outeryes+

20 / 600

65 mm
2

94 mm

20 ml

yes

20 / 480

Macroplate / Square Petri Dish /OneWell Plate/

With vents/

Manufactured from crystal clear polystyrene/

Optimised space requirement compared to rounddishesnoncytotoxic

174 /Item no.

FeatureWellformat657102

microplate
format6688102square670102

microplate
formatwww.gbo.com

Length127.8 mm 85.5 mm

Rawmaterial

PS

ProductcolourTotal
volume(well)

Lidl clear16 mlyes, condensationrings

120 mml clear127.8 mm 85.5 mm

l clear

120 mm1

Width113.7 ml

Vent
nock

Qty. inner/ outer2 / 100

yes

yes

10 / 240yes

yes

8 / 32

Microbiology
Dishes / Plates / Others

Swab Tube
Cotton Swab/

Suitable for taking non-human bacteriological,
serological or cytological samples in veterinary
research/

For hygienic controls in food industry as well as inenvironmental sampling

STERILE

Sterile: +Item no.

Description

FeatureHeightØ

SterileQty. inner / outer

420161

Swab Tubeswab tube, polystyrol

110 mm

16 mm+

1.300 / 1.300

420180Swab Tubeswab tube, polystyrol

110 mm

16 mm+

1 / 1.000

421161

Swab Tubeswab tube, polystyrol

152 mm

16 mm+- /1.000

421180Swab Tubeswab tube, polystyrol

152 mm

16 mm+

1 / 700

421084

Cotton Swab+

1 / 1.400

145 mmDisposable Inoculation Loops / Needles/

Needles ideal for picking single colonies/

Flexible loops for easier collection and inoculation/

Colour coding for volume differentiation

STERILE

Length: 200 mm, Sterile: +Item no.

Description

Product colour

VolumeSterileQty. inner / outer

731101

inoculation loop

l white

1 µl+

50 / 2.000

731161

inoculation loop

l white

1 µl+

1 / 600

731165

inoculation loop

l white

1 µl+

10 / 3.000

731170inoculation loop

l blue10 µl+

50 / 2.000

731171

inoculation loop

l blue10 µl+

1 / 600

731175

inoculation loop

l blue10 µl+

10 / 3.000

www.gbo.com

/ 175

Microbiology
Dishes / Plates / Others

Item no.

Description

Product colour

VolumeSterileQty. inner / outer

731180inoculation needle

l yellow+

50 / 2.000

731181

inoculation needle

l yellow+

1 / 600

731185

inoculation needle

l yellow+

1 / 3.000

Disposal Bags
Disposal bags made of polypropylene forsterilisation in the steam autoclave. For usersof hot-air sterilisers, disposal bags made ofpolyamide are suitable for use up to +160 °C.

Foil thickness: 0.05 mm
LengthWidthNominalcapacity

Suitable for steam
autoclaves

500 mm

300 mm

10 l

500 mm

300 mm10 l643401500 mm

300 mm10 l644201780 mm

400 mm

30 l

780 mm

400 mm

30 l

780 mm

400 mm

30 l

780 mm

600 mm65 l646203780 mm

600 mm65 l

646401780 mm

600 mm65 l649201

1,100 mm

700 mm130 l

1,100 mm

700 mm130 l

1,100 mm

700 mm130 lItem no.

Feature643201643203644203

Imprint "Biohazard"

Imprint "Biohazard"

644401646201649203649401

176 /www.gbo.com

Imprint "Biohazard"

Imprint "Biohazard"

Suitablefor hot air
sterilizers

Material

Qty. inner /
outer

yes

PP

500 / 500

yesPP

500 / 500PA

500 / 500

yesPP

500 / 500

yesPP

500 / 500PA

500 / 500

yesPP

500 / 500

yesPP

1 / 500

PA

300 / 300

yesPP

350 / 350

yesPP

350 / 350PA

200 / 200

yes

yes

yes

yes

Microbiology
Dishes / Plates / Others

www.gbo.com

/ 177

Greiner Bio-One offers a comprehensive range of tubes andmultipurpose beakers for various applications. The vessels areavailable in different materials, with and without caps and withlabelling options.

178 /

www.gbo.com

Tubes / Multipurpose Beakers
﻿

TUBES / MULTIPURPOSE BEAKERS/ Tubes without closures ����������������������180
Tube Polystyrene�������������������������������������������� 181
Tube Polypropylene���������������������������������������� 181
Grip Stopper ������������������������������������������������� 182
Tube Polypropylene for Storage Box�������������� 182/ Tubes with closures ���������������������������� 183
Centrifuge Tubes Polypropylene - 15 ml��������� 184
Centrifuge Tubes Polypropylene - 50 ml�������� 185
CELLreactor ������������������������������������������������� 186
Tubes with closures Polystyrene������������������� 186
Tube Two-position Cap�����������������������������������187/ Separation Tubes ������������������������������� 188
Leucosep 12 ml and 50 ml������������������������������ 189
OncoQuick® ��������������������������������������������������� 189/ Multipurpose Containers / Beakers �����190
Multipurpose Containers Polystyrene������������� 191
Multipurpose Beaker Polypropylene��������������� 191
Containers for Plant Cultures Polystyrene����� 192
Drosophila Containers ���������������������������������� 192
Ceaprene stopper ����������������������������������������� 193

www.gbo.com

/ 179

Tubes / Multipurpose Beakers
Tubes without closures/

For the max. relative
centrifugal force (RCF)
and the chemical/thermal resistance of ourtubes please refer tothe technical appendix.

/

With round or conicalbottom/With or without skirt/

Available in polystyrene
or polypropylene

TUBES WITHOUT CLOSURESThe range of Greiner Bio-One
tubes is very versatile andmeets a wide variety of different demands.
Our tubes are made of different
materials: Polystyrene (PS) is
ideally suited for optical measurements as a result of its highclarity. Polypropylene (PP) is re-

180 /www.gbo.com

commended for the storage ofchemical and biological samples due to its thermal, mechanical and chemical resistance.
Greiner Bio-One tubes without
closure are available with roundbottom. In addition, it is possible to order appropriate closuresin the form of grip stoppers andscrew caps.

Tubes / Multipurpose Beakers
Tubes without closures

Tube
Polystyrene/High transparency/

Available in different sizes and packaging units

Raw material: PS, Bottom shape: round
Item no.

HeightØWorking volume

Nominal volume

Qty. inner / outer

112101

55 mm

12 mm

≤3 ml

4 ml

240 / 3.600

115101

75 mm

12 mm

≤4 ml

5 ml

250 / 2.000

136101

100 mm

14 mm

≤8 ml

10 ml

1.400 / 1.400

160101

100 mm

16 mm≤10.5 ml

12 ml

1.600 / 1.600

187101

100 mm

17 mm≤12 ml14 ml

1.500 / 1.500

169101152 mm

16 mm≤18 ml20 ml

1.500 / 1.500

Tube
Polypropylene/

Suited for sample storage/

Thermal, chemical and mechanical stability/

Available in different sizes and packaging units

Raw material: PP, Bottom shape: round
Item no.

HeightØWorking volume

Nominal volume

Qty. inner / outer

112201

55 mm

12 mm

≤3 ml

4 ml

240 / 3.600

115201

75 mm

12 mm

≤4 ml

5 ml

250 / 2.000

160201

100 mm

16 mm≤10 ml12 ml- /1.600

187201

100 mm

17 mm≤12 ml14 ml

1.500 / 1.500

www.gbo.com

/ 181

Tubes / Multipurpose Beakers
Tubes without closures

Grip Stopper/

Made of high-grade polyethylene

Description: Grip stopper, Raw material: PE
Item no.

Product colour

Suitable for tubes Ø

Qty. inner / outer

303321l natural

12 mm

500 / 20.000

310321l natural

16 mm

100 / 8.000

Tube
Polypropylene for Storage Box/

Tubes for storage box item no. 975502

Height: 44 mm, Ø: 8.5 mm, Raw material: PP, Bottom shape: round, Working volume: ≤1 ml, Nominal volume: 1.3 ml

Item no.

182 /Feature102201

Tube102261

tube chain with attached strip cap

102270tube chain with attached strip cap

www.gbo.com

SterileQty. inner / outer

1.000 / 1.000+- /1.000- /1.000

Tubes / Multipurpose Beakers
Tubes with closures/

For the max. relative
centrifugal force (RCF)
and the chemical/thermal resistance of ourtubes please refer tothe technical appendix.

TUBES WITH CLOSURESGreiner Bio-One tubes withclosures are made of different
materials: Polystyrene (PS) is
ideally suited for optical measurements as a result of its highclarity. Polypropylene (PP) is
recommended for the storageof chemical and biological samples due to its thermal, mechanical and chemical resistance.
Greiner Bio-One tubes without

closure are available with roundor with conical bottom as well aswith or without skirt. They areavailable in sterile or non-sterileversions. The product range iscompleted by tubes with a twoposition cap. This special capenables ventilation or an airtightclosure of the tube, dependingon the position of the stopper.

www.gbo.com

/ 183

Tubes / Multipurpose Beakers
Tubes with closures

Centrifuge Tubes
Polypropylene - 15 ml

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Optimal mechanical, thermal and chemical stability/

Available with blue or white cap/

Triple-packed options available/

Light protection tubes for light-sensitive materials
and reactions

nonpyrogenic

Height: 120 mm, Ø: 17 mm, Support skirt: no, Graduation: yes, Writing field: yes, Raw material: PP,
Bottom shape: conical, Working volume: ≤14 ml, Nominal volume: 15 ml, Cap design: screw cap, Sterile: +Product colour

Cap colour

Colour writing
field

Graduation
colourType ofpackaging

SterileQty. inner /outer188261-Nl naturall bluel whitel bluerack+

50 / 500

188271-Nl naturall bluel whitel bluebag+

50 / 1.000

188271-TRI

l naturall bluel whitel bluetriple packed+

5 / 500

188281l naturall whitel whitel bluerack+

50 / 500

188285l naturall whitel whitel bluebag+

100 / 700

Item no.

184 /Feature188283light protection tube

l brownl bluel whitel bluerack+

50 / 500

188280light protection tube

l brownl bluel whitel bluebag+

100 / 1.000

www.gbo.com

Tubes / Multipurpose Beakers
Tubes with closures

Centrifuge Tubes
Polypropylene - 50 ml

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Optimal mechanical, thermal and chemical stability/

Available with blue or white cap/

Triple-packed options available/

Light protection tubes for light-sensitive materials
and reactions

nonpyrogenic

Height: 115 mm, Ø: 30 mm, Graduation: yes, Writing field: yes, Raw material: PP, Bottom shape: conical,
Working volume: ≤50 ml, Nominal volume: 50 ml, Cap design: screw cap, Sterile: +Supportskirt

Product colour

Cap colour

Colourwriting field

Graduation
colourType ofpackaging

SterileQty. inner /outer210261yes

l naturall bluel whitel bluebag+

25 / 450

210270yes

l naturall bluel whitel bluerack+

25 / 300

227261nol naturall bluel whitel bluebag+

20 / 500

227261-Nnol naturall bluel whitel bluebag+

25 / 500

227270nol naturall bluel whitel bluerack+

25 / 300

227270-Nnol naturall bluel whitel bluerack+

25 / 250

227261-TRI

nol naturall bluel whitel bluetriplepacked

+5 / 250

227285nol naturall whitel whitel bluebag+

20 / 500

227281nol naturall whitel whitel bluerack+

25 / 300

Item no.

Feature227283light
protection
tube

no

l brownl bluel whitel bluerack+

25 / 300

227280light
protection
tube

no

l brownl bluel whitel bluebag+

20 / 500/ Please contact your local distribution partner for the availability of 227261-N / 227270-N or 227261 / 227270.

www.gbo.com

/ 185

Tubes / Multipurpose Beakers
Tubes with closures

CELLreactor
15 ml and 50 ml polypropylene tube with filter
screw cap

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

For cultivation of suspension cells and expansionof aerobic microorganisms/

Facilitates a high number of parallel experiments/

Maximal sterility and excellent gas exchange/

Conical tube design and in-tube harvest

nonpyrogenic

Graduation: yes, Writing field: yes, Raw material: PP, Bottom shape: conical, Cap design: filter screw cap, Sterile: +Item no.

HeightØ

188241

120 mm

17 mm

227245115 mm

30 mm

Cap colour

Working volume

Nominal volume

SterileQty. inner / outerl blue1 ml - 5 ml

15 ml

+

20 / 500l blue1 ml - 35 ml

50 ml

+

20 / 500/ Application Note: Cultivation of Suspension and Hybridoma Cells in CELLSTAR® CELLreactor Tubes (F073918)/ Application Note: Superior protein yields in suspension CHO cells using FectoPRO™-mediated transient transfection in CELLSTAR®
CELLreactor (F073926)

Tubes with closures
Polystyrene/High transparency/

Available with screw cap or bayonet cap in blue /black / white/With or without skirt/

In bulk or rack packaging

Raw material: PS

186 /Item no.

HeightØSupportskirt

Graduation

Writing
field

Bottomshape

Cap colour

Working
volumeNominalvolumeCap design Sterile

Qty. inner /outer164180

100
mm

16.8
mm

yes

-

no

conicall white≤12 ml

12 mlbayonetcap

1.300 /
1.300

164161

100
mm

16.8
mm

yes

-

no

conicall blue≤12 ml

12 mlbayonetcap

163177

100
mm

17
mm

no

-

no

round

l black≤12 ml

12 ml

screw
cap

188161

120
mm

17
mm

no

yes

yes

conicall blue≤14 ml

15 ml

screw
cap

www.gbo.com

+

25 /
1.000
900 /
900

+

50 / 500

Tubes / Multipurpose Beakers
Tubes with closures

Item no.

HeightØSupportskirt

Graduation

Writing
field

Bottomshape

Cap colour

Working
volumeNominalvolume188171

120
mm

17
mm

no

yes

yes

conicall blue≤14 ml

15 ml

screw
cap

+

100 /
1.000

186171

120
mm

17
mm

no

-

no

round

l blue≤15 ml

15 ml

screw
cap

+

100 /
1.000

Cap design Sterile

Qty. inner /outer/ Item no. 163177 is also available as polypropylene version with white screw cap (item no. 163270).

Tube
Two-position Cap
The two-position cap enables ventilation or an
airtight closure of the tube, depending on theposition of the stopper.

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

nonpyrogenic

Bottom shape: round, Cap design: Two-position Cap, Sterile: +HeightØ

Graduation

Writingfield

Rawmaterial

Working
volumeNominalvolumeSterileQty. inner / outer115261

75 mm

12 mm

yes

no

PP

≤4 ml

5 ml

+

1 / 1.000

115262

75 mm

12 mm

yes

no

PP

≤4 ml

5 ml

+

25 / 2.000

12016175 mm

12.4 mm-noPS

l blue≤4 ml

4.5 ml+1 / 1.000

12018075 mm

12.4 mm-noPS

l blue≤4 ml

4.5 ml+25 / 2.000

187261

95 mm

18 mm

yes

yes

PP

≤12 ml14 ml+

1 / 800

187262

95 mm

18 mm

yes

yes

PP

≤12 ml14 ml+

25 / 1.000

191161

95 mm

18 mm

-

no

PS

≤12.5 ml

14 ml+

1 / 750

191180

95 mm

18 mm

-

no

PS

≤12.5 ml

14 ml+

25 / 1.000

Item no.

Graduation
colourwww.gbo.com

/ 187

Tubes / Multipurpose Beakers
Separation Tubes/

For further information, please refer to our
website: www.gbo.com/

Leucosep for the
separation oflymphocytes/mononuclear cells fromblood/bone marrow/

OncoQuick® for theenrichment ofdisseminated,circulating tumour cells
from peripheral blood

SEPARATION TUBES
Different separation techniques
can be used to enrich certain
particles (DNA, RNA, proteins,
cells etc.) specifically from complex biological mixtures such ascell and tissue homogenates,
blood or urine, so that they canthen be selectively investigated.
The separation of cells by density gradient centrifugation has
proven to be the most often usedmethod.
Leucosep has been developed for optimal separationof lymphocytes and peripheral mononuclear cells (so-called PBMCs) from human whole
blood and bone marrow by

188 /www.gbo.com

means of density gradient centrifugation. The key feature of
Leucosep is the porous barrier
incorporated into the centrifuge
tube made of highly translucent
polypropylene.
OncoQuick® is a simple-to-use,
rapid and efficient system forthe enrichment of circulating
tumour cells that are released
into the blood by a solid epithelial tumour or malignant melanoma. OncoQuick ® combines
the advantages of cell separation by density gradient centrifugation with recovery rates thatare comparable with immunobead methods.

Tubes / Multipurpose Beakers
Separation Tubes

Leucosep
12 ml and 50 ml
Efficient separation of lymphocytes andmononuclear cells from peripheral blood andbone marrow.

noncytotoxic/

Easy to fill/

No re-contamination with unwanted red blood cells/

PBMC isolation from whole blood in 15 min

nonpyrogenic

Type of sample: blood
Item no.

Separation medium

Bottom shape

Sample volume

pre-filled with Leucosep separation mediumround

3 - 8 ml

50 / 500

163289round

3 - 8 ml

50 / 500

163290round

3 - 8 mlconical15 - 30 ml

25 / 250

227289conical15 - 30 ml

25 / 300

227290conical15 - 30 ml

163288227288pre-filled with Leucosep separation medium

Sterile++Qty. inner / outer

50 / 500

25 / 300/ For IFU please refer to our website: www.gbo.com

OncoQuick®
Enrichment of disseminated, circulating
tumour cells from peripheral blood.
/

Time request approx.45 minutes/

Reproducible recovery: > 70 %/No additional laboratory equipment required/

Tubes are pre-filled with separation medium

noncytotoxic

Sample volume: 15 - 30 ml, Type of sample: blood
Item no.

Cap colour

Qty. inner / outer

227255l blue1/1227250l blue10 / 10/ For IFU please refer to our website: www.gbo.com

www.gbo.com

/ 189

Tubes / Multipurpose Beakers
Multipurpose Containers / Beakers/

For further information, please refer to our
website: www.gbo.com/

Multipurpose
containers inpolystyrene orpolypropylene/

Polystyrene containers
for plant culture/

Polystyrene drosophila
containers

MULTIPURPOSE CONTAINERS / BEAKERS
Multipurpose containers or beakers can be used for a variety ofdifferent applications. They canbe applied universally for academic and non-human medical
purposes. Multipurpose containers are made of polystyrene orpolypropylene and are available
in different sizes. They feature
either plastic or metal caps andcan be supplied with or without
plain or printed label.
Containers for plant culture are
made of a very clear material
which ensures maximum lighttransmission and thus rapid andsuccessful growth of plant cul-

190 /www.gbo.com

tures. These culture containers
are not only suitable for the proliferation of plant cultures but
can also be used as transport
containers and are available withor without lids. Our drosophila
containers are ideally suitedfor the cultivation of Drosophilamelanogaster. The bottom partof the container is made of polystyrene. Drosophila containers
can be supplied with a separately orderable ceaprene stopper.
This stopper is gas-permeable and made of water-repellent
material.

Tubes / Multipurpose Beakers
Multipurpose Containers / Beakers

Multipurpose Containers
Polystyrene/

Manufactured from crystal clear polystyrene/

Available in different sizes/

Can be used universally for academic andnon-human medical purposes/With or without plain or printed label/

Plastic or metal cap

Raw material: PS, Cap design: screw cap
HeightØSupportskirt

Bottomshape

Cap colour

Working
volumeNominalvolumeCapmaterial

201150

91 mm

25 mm

yes

conicall white≤23 ml30 ml

plastics

201170

91 mm

25 mm

yes

conicall white≤23 ml30 ml

plastics

Item no.

FeatureSterileQty. inner /outer

400 / 400+

400 / 400

201152

with plain
label

91 mm

25 mm

yes

conicall white≤23 ml30 ml

plastics

201172

with plain
label

91 mm

25 mm

yes

conicall white≤23 ml30 ml

plastics

224170

77 mm

49 mm

l silver≤80 ml 100 ml

metal

20 / 160

225170115 mm49 mm

l silver≤150 ml 150 ml

metal

20 / 120225180with plain
115 mm
label

49 mm

l silver≤150 ml 150 ml

metal

20 / 120

400 / 400+

400 / 400

Multipurpose Beaker
Polypropylene/

Can be used universally for non human medical
purposes/

Sterile and non-sterile

Height: 70 mm, Ø: 55 mm, Graduation: yes, Working volume: ≤120 ml, Nominal volume: 120 ml, Cap design: screw cap

Item no.

FeatureProduct colour

Cap colour

SterileQty. inner / outer+

300 / 300

724410l naturall yellow

724411l naturall white724412l naturall white+300 / 300l naturall white+300 / 300

724413

Tamper proof label

300 / 300

www.gbo.com

/ 191

Tubes / Multipurpose Beakers
Multipurpose Containers / Beakers

Item no.

FeatureProduct colour

724414Cap colour

l natural

SterileQty. inner / outer+

300 / 300l red

/ Item No., lot number and expiry date are printed on the screw cap of the beaker to provide additional information and improve traceability

Containers for Plant Cultures
Polystyrene
The use of a very clear material ensuresmaximum light transmission and thus rapid
growth. These culture containers are not only
suitable for the proliferation of plant cultures
but can also be used as transport containers.

Height: 100 mm, Raw material: PS
Item no.

DescriptionØWorking volume

Nominal volume

Cap design

SterileQty. inner / outer

960161

container

53 mm≤150 ml

175 mlpull cap+

4 / 300

968177

container, bottom part

68 mm

≤300 ml

330 ml192 / 192

/ Also available with lid as sterile version.

Drosophila Containers/

Ideally suited for the cultivation of Drosophilamelanogaster/

Choice of different sizes/

Bottom part of container made of polystyrene

Description: Drosophila Containers, Raw material: PS
Item no.

HeightØ20510164 mm

217101960177

Working volume

Nominal volume

Qty. inner / outer

27 mm

28 ml

1.500 / 1.500

82 mm

36 mm

68 ml

605 / 605

100 mm

53 mm

175 ml315 / 315

≤150 ml/ Can be supplied with a separately orderable ceaprene stopper. This stopper is gas-permeable and made of water-repellent material.

192 /www.gbo.com

Tubes / Multipurpose Beakers
Multipurpose Containers / Beakers

Ceaprene stopper

Item no.

/Gas-permeable/

Made of water-repellent material

Suitable for tubes Ø

Qty. inner / outer

35407027 mm- /1.500

33007036 mm- /60533207053 mm- /315www.gbo.com

/ 193

For sample freezing and storage, Greiner Bio-One offers acomprehensive product portfolio including Cryo.s tubes, racks aswell as electronic equipment for tube closure and sample tracking.
With the brand Cryo.s Greiner Bio-One combines more than 30 yearsof experience in the manufacturing of cryogenic tubes with latest
technology and innovation.

194 /www.gbo.com

Cryotechnics
﻿

CRYOTECHNICS/ Cryo.s Cryo Tubes������������������������������� 196
Cryo.s - 1 ml ����������������������������������������������������197
Cryo.s - 2 ml �������������������������������������������������� 198
Cryo.s - 4 ml �������������������������������������������������� 199
Cryo.s - 5 ml ������������������������������������������������� 200
Support Rack ����������������������������������������������� 200
Cryo storage box ������������������������������������������� 201/ Cryo.s with Barcode �������������������������� 202
Cryo.s with Barcode —Preproduced unique codes����������������������������203
Cryo.s with Barcode —Customised Codes�����������������������������������������203
48-way Datamatrix Cryo Rack ���������������������� 204
81-way Datamatrix Cryo Rack ����������������������� 204/ Cryo.s Biobanking Tubes ������������������� 205
Cryo.s Biobanking Tubes 300 µl���������������������206
Cryo.s Biobanking Tubes 300 µl���������������������207
Cryo.s Biobanking Tubes 600 µl���������������������208
Cryo.s Biobanking Tubes 600 µl���������������������209
Cryo.s Biobanking Tubes 1000 µl�������������������� 210
Cryo.s Biobanking Tubes 1000 µl��������������������� 211
Screw Caps Biobanking Tubes����������������������� 212
96-way Datamatrix Cryo Rack ����������������������� 212
Cryo.s Decapper ������������������������������������������� 213
Cryo.s Rack Scanner ������������������������������������� 214

www.gbo.com

/ 195

Cryotechnics
Cryo.s Cryo Tubes/

For a freezing and thawing protocol as well
as safety advisory for
Cryo.s please refer tothe technical appendix.

/

CE-marked/

USP class VI certified
medical grade
polypropylene/

Sterilised applying
an ISO 11137 validated
irradiation procedureyielding an
SAL level of 10 – 6/

Suited for storage inliquid nitrogen vapour
phase (-196 °C)

CRYO.S
CRYO TUBES
Cryo.s, Cryo.s with datamatrix and linear barcode andCryo.s biobanking tubes are forstorage of tissue, cells, fungi,
bacteria, spores, cellular extracts or body fluids at ultralow temperature for researchand development purposes aswell as in-vitro diagnostics. They
must not be stored in the liquid
phase of liquid nitrogen, but onlyin the gas phase above. Cryo.s
tubes are not intended for any
application in the context of reproductive medicine.
An outstanding feature of all
Cryo.s is the utilisation of a
USP class VI certified, medical
grade polypropylene for tube

196 /www.gbo.com

manufacturing. This material
does not release significant
amounts of leachables, hence
stored samples stay clean andfree of contamination. Sterile
product versions are sterilised
applying an ISO 11137 validated
irradiation procedure yielding an
SAL level of 10 –6.
The portfolio of Cryo.s tubes
includes cryogenic tubes withdifferent volumes, different
base forms as well as several cap
colours. Cryo.s offer several features for the identification andlabelling of individual samples,
to include coloured screw caps,
a white, scratch-resistant writing area and barcoding options.

Cryotechnics
Cryo.s Cryo Tubes

Cryo.s - 1 ml

STERILE

IVD

0197

FREE OFdetectable
DNase

FREE OFdetectable
human DNA/High thermal resistance/

Cryo.s with internal thread have a screw cap withsilicone gasket/

Cap inserts item no. 304134 (50 pieces per bag)/

USP class VI certified medical grade polypropylene

FREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Height: 42 mm, Ø: 12.5 mm, Starfoot: yes, Raw material: PP, Bottom shape: conical, Working volume: ≤1.2 ml,
Cap design: screw cap, Sterile: +Item no.

BarcodeGraduation

Writing field

Cap colour

Thread type123261nonono

l natural

123263

no

yes

yes

123263-TRI

no

yes

123277

no123278Triple-packed

SterileQty. inner / outerinternal+

100 / 500l naturalinternal+

100 / 500

yes

l naturalinternal+

10 / 200

yes

yes

l greeninternal+

100 / 500

no

yes

yes

l yellowinternal+

100 / 500

123279

no

yes

yes

l blueinternal+

100 / 500

123280

no

yes

yes

l red

internal+

100 / 500

yes

/ with 150 inserts per casewww.gbo.com

/ 197

Cryotechnics
Cryo.s Cryo Tubes

Cryo.s - 2 ml

0197

STERILE

IVD

FREE OFdetectable
DNase

FREE OFdetectable
human DNA/High thermal resistance/

Cryo.s with internal thread have a screw cap withsilicone gasket/

Cap inserts item no. 304134 (50 pieces per bag)/

USP class VI certified medical grade polypropylene

FREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Raw material: PP, Bottom shape: round, Cap design: screw cap, Sterile: +HeightØ

Starfoot

BarcodeGraduation

Writingfield

Cap colour

Working
volumeThreadtype

121261

48
mm

12.5
mm

no

no

-

no

l natural≤2 ml

121263

48
mm

12.5
mm

no

no

yes

yes

l natural

121277

48
mm

12.5
mm

no

no

yes

yes

121278

48
mm

12.5
mm

no

no

yes

121279

48
mm

12.5
mm

no

no

121280

48
mm

12.5
mm

no

122261

48
mm

12.5
mm

12226348mm122263-TRI

SterileQty. inner /outerinternal+

100 / 500

≤2 ml

internal+

100 / 500

l green≤2 mlinternal+

100 / 500

yes

l yellow≤2 mlinternal+

100 / 500

yes

yes

l blue≤2 mlinternal+

100 / 500

no

yes

yes

l red

≤2 ml

internal+

100 / 500

yes

no

-

no

l natural≤2 mlinternal+

100 / 500

12.5
mm

yes

no

yes

yes

l natural≤2 mlinternal+

100 / 500

48
mm

12.5
mm

yes

no

yes

yes

l natural≤2 mlinternal+

10 / 200

122277

48
mm

12.5
mm

yes

no

yes

yes

l green≤2 mlinternal+

100 / 500

122278

48
mm

12.5
mm

yes

no

yes

yes

l yellow≤2 mlinternal+

100 / 500

122279

48
mm

12.5
mm

yes

no

yes

yes

l blue≤2 mlinternal+

100 / 500

122280

48
mm

12.5
mm

yes

no

yes

yes

l red

≤2 ml

internal+

100 / 500

126261

47
mm

12.4
mm

yes

no

-

no

l natural≤2.2 ml external+

100 / 500

126263

47
mm

12.4
mm

yes

no

yes

yes

l natural≤2.2 ml external+

100 / 500

126263-TRI

47
mm

12.4
mm

yes

no

yes

yes

l natural≤2.2 ml external+

10 / 200

Item no.

198 /www.gbo.com

Triplepacked

yes

yes

Cryotechnics
Cryo.s Cryo Tubes

Item no.

HeightØ

Starfoot

BarcodeGraduation

Writingfield

Cap colour

Working
volume126277

47
mm

12.4
mm

yes

no

yes

yes

l green126278

47
mm

12.4
mm

yes

no

yes

yes

126279

47
mm

12.4
mm

yes

no

yes

126280

47
mm

12.4
mm

yes

no

yes

Threadtype

Triplepacked

SterileQty. inner /outer≤2.2 ml external+

100 / 500

l yellow≤2.2 ml external+

100 / 500

yes

l blue≤2.2 ml external+

100 / 500

yes

l red

≤2.2 ml external+

100 / 500

/ with 150 inserts per caseCryo.s - 4 ml

0197

FREE OFdetectable
DNase

STERILE

IVD

FREE OFdetectable
human DNA/High thermal resistance/

Cap inserts item no. 304134 (50 pieces per bag)/

USP class VI certified medical grade polypropylene

FREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Description: Cryo.s 4 ml, Height: 83 mm, Ø: 12.4 mm, Starfoot: yes, Raw material: PP, Bottom shape: round,Working volume: ≤4 ml, Cap design: screw cap, Sterile: +Item no.

BarcodeGraduation

Writing field

Cap colour

Thread type

SterileQty. inner / outer

127261nonono

l natural

external+

50 / 300

127263

no

yes

yes

l natural

external+

50 / 300

127277

no

yes

yes

l greenexternal+

50 / 300

127278

no

yes

yes

l yellowexternal+

50 / 300

127279

no

yes

yes

l blueexternal+

50 / 300

127280

no

yes

yes

l red

external+

50 / 300/ with 100 inserts per casewww.gbo.com

/ 199

Cryotechnics
Cryo.s Cryo Tubes

Cryo.s - 5 ml

0197

FREE OFdetectable
DNase

STERILE

IVD

FREE OFdetectable
human DNA/High thermal resistance/

Cryo.s with internal thread have a screw cap withsilicone gasket/

Cap inserts item no. 304134 (50 pieces per bag)/

USP class VI certified medical grade polypropylene

FREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Description: Cryo.s 5 ml, Height: 86 mm, Ø: 12.5 mm, Starfoot: no, Raw material: PP, Bottom shape: round,Working volume: ≤4.5 ml, Cap design: screw cap, Sterile: +Item no.

BarcodeGraduation

Writing field

Cap colour

Thread type

SterileQty. inner / outer124261no-nol naturalinternal+

50 / 300

124263

no

yes

yes

l naturalinternal+

50 / 300

124273

no

yes

yes

l red

internal+

50 / 300

124275

no

yes

yes

l greeninternal+

50 / 300

124276

no

yes

yes

l yellowinternal+

50 / 300

124274

no

yes

yes

l blueinternal+

50 / 300/ with 100 inserts per case

Support Rack/

Suitable for Cryo.s with starfoot base (Item No.
122XXX, 123XXX, 126XXX, 127XXX)/

Improved handling since the tubes can be opened
with one hand/

Rubber base to prevent slipping/

Offers space for up to 40 Cryo.s

Description: support rack for one-hand operation, Height: 22 mm, Length: 200 mm, Width: 100 mm, Raw material: PC

Item no.

802501200 /www.gbo.com

Rack colour
l blueQty. inner / outer1 / 50Cryotechnics
Cryo.s Cryo Tubes

Cryo storage box
The box material polypropylene is very
temperature and shock resistant, thus
allowing storage temperatures as low
as -90 °C.
/

Holds 81 Cryo.s sample tubes/

Transparent lid for optimum visibility of box content

Description: Cryo storage box, Height: 51 mm, Length: 126.5 mm, Width: 126.5 mm, Raw material: PP

Item no.

Rack colour

Qty. inner / outer

802202l natural

5 / 20802203l red

5 / 20802204l blue5 / 20802206l yellow

5 / 20802225l green

5 / 20/ The indicated height refers to the Cryo Storage Box filled with 2 ml Cryo.s and covered with a lid.

www.gbo.com

/ 201

Cryotechnics
Cryo.s with Barcode

CRYO.S WITH BARCODE
Cryo.s, Cryo.s with datamatrix
and linear barcode and Cryo.s
biobanking tubes are for storage of tissue, cells, fungi, bacteria, spores, cellular extracts orbody fluids at ultra-low temperature for research and development purposes as well as in-vitro diagnostics. They must not
be stored in the liquid phase of
liquid nitrogen, but only in the

202 /www.gbo.com

gas phase above. Cryo.s tubes
are not intended for any application in the context of reproductive medicine.
With Cryo.s with Datamatrix andthe accessory racks in 48-way
and 81-way formats, Greiner
Bio-One expands its portfolioby optimum solutions for semiautomated and automated sample handling and storage.

/

For further information, please refer to our
website: www.gbo.com/

Datamatrix code
ECC200 on the tube
bottom with reliable
Reed-Solomon error
correction/

Linear barcode type
128 with plain text on
the tube side/

Application of barcodes and datamatrix
codes by means ofinnovative laser
technology/

100 % controlled codereadability/

Suited for storage inliquid nitrogen vapour
phase (-196 °C)/

Coded tubes are suitable for airfreight(IATA conformity)

Cryotechnics
Cryo.s with Barcode

Cryo.s with Barcode— Preproduced unique codes

0197

IVD

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNA/

Pre-produced unique datamatrix code ECC 200
with 14 x 14 dots on tube bottom, additional linear
barcode type 128 with human readable text on tube
side/

Highest code resistance against chemicals, mechanical stress and extremely low storage temperatures (down to -196 °C)

FREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Starfoot: yes, Barcode type: linear barcode and datamatrix code, Raw material: PP, Cap design: screw cap, Sterile: +Working
volumeThread type

SterileQty. inner /outerl natural≤1.2 mlinternal+

100 / 500

round

l natural≤2 mlinternal+

100 / 500

yes

round

l natural≤2.2 mlexternal+

100 / 500

yes

round

l natural≤4 mlexternal+

50 / 300

Item no.

HeightØ

BarcodeBottom shape123263-2DG

42 mm

12.5 mmyes

conical122263-2DG 48 mm

12.5 mmyes

126263-2DG

47 mm

12.4 mm

127263-2DG 83 mm

12.4 mm

Cap colour/ Other screw cap colours on request.

Cryo.s with Barcode— Customised Codes

0197

IVD

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNA/

Customised datamatrix code ECC 200 with 14 x 14
dots on tube bottom, additional customised linear
barcode type 128 with human readable text on tube
side/

Highest code resistance against chemicals, mechanical stress and extremely low storage temperatures (down to -196 °C)

FREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Starfoot: yes, Barcode type: linear barcode and datamatrix code, Raw material: PP, Cap design: screw cap, Sterile: +Item no.

HeightØ

BarcodeBottomshape

Cap colour

Working
volumeNominalvolumeThread type

SterileQty. inner /outer123263-2D3

42 mm

12.5 mmyes

conicall natural≤1.2 ml

1 ml

internal+

100 / 500

122263-2D3

48 mm

12.5 mm

yes

round

l natural

≤2 ml

2 ml

internal+

100 / 500

126263-2D1

47 mm

12.4 mm

yes

round

l natural≤2.2 ml

2 ml

external+

100 / 500

www.gbo.com

/ 203

Cryotechnics
Cryo.s with Barcode

Item no.

HeightØ

BarcodeBottomshape

Cap colour

Working
volumeNominalvolumeThread type

SterileQty. inner /outer127263-2D1

83 mm

12.4 mm

yes

round

l natural

≤4 ml

4 ml

external+

50 / 300/ Custom barcode orders have a minimum order quantity of 10,000 tubes per order and request.
/ Other screw cap colours on request.
/ Barcodes to be specified in order form F071004.

48-way Datamatrix Cryo Rack/

SLAS-standard format/

Automation compatible/

Suitable for storage in vapour phase above liquid
nitrogen/

Barcoded versions on request

Length: 85.5 mm, Width: 127.8 mm, Raw material: PP, Lid type: with lid
Item no.

Description

HeightBarcode803202

low rack

52.5 mmno803270high rack

88.5 mmnoRack colour

Suitable for Cryo.s

Qty. inner / outerl black

1 / 2 ml- /20

l black

4 ml

- /15

81-way Datamatrix Cryo Rack/

Shock-proof and temperature-resistantpolycarbonate/

Stacking feature and rotation stoppers/

133 x 133 mm footprint for storage in classic
freezer setups/

Suitable for storage in vapour phase above liquid
nitrogen/

Barcoded versions on request

Length: 132.4 mm, Width: 132.4 mm, Raw material: PC, Lid type: with lid
Item no.

204 /Description

Height802576

low rack

52 mm

802506high rack

88.1 mm

www.gbo.com

Rack colour

Suitable for Cryo.s

Qty. inner / outerl black

1 / 2 ml1 / 10l black

4 ml

1 / 10Cryotechnics
Cryo.s Biobanking Tubes/

Forum No. 21: Sample
Storage Tubes as Quality-Critical Components in Biobanking(F073072)/

You can find more
information about thisproduct in our
webshop.

CRYO.S BIOBANKING TUBES/

Application of barcodes and datamatrix
codes by means ofinnovative laser
technology/

100 % controlled codereadability/

Coded tubes are
suitable for airfreight(IATA conformity)/

Suited for storage at-20 °C, -80 °C and invapour phase above
liquid nitrogen

With Cryo.s biobanking tubes,
Greiner Bio-One offers an ideal
solution for the efficient storage of biological samples inlarge-scale biorepositories.
Cryo.s biobanking tubes areavailable with working volumesof 235 μl, 580 μl and 975 μl andoffered in highly automationfriendly 96-way racks. The innovative design of tubes and racks
allows for a very space-efficientstorage with up to 30 % better
utilisation of storage space infreezers or liquid nitrogen tanks.

Cryo.s, Cryo.s with datamatrix
and linear barcode and Cryo.s
biobanking tubes are for storage of tissue, cells, fungi, bacteria, spores, cellular extracts orbody fluids at ultra-low temperature for research and development purposes as well as in-vitro diagnostics. They must not
be stored in the liquid phase of
liquid nitrogen, but only in thegas phase above. Cryo.s tubes
are not intended for any application in the context of reproductive medicine.

www.gbo.com

/ 205

Cryotechnics
Cryo.s Biobanking Tubes

Cryo.s Biobanking Tubes sterile
300 µl0197

FREE OFdetectable
DNase

STERILE

IVD

FREE OFdetectable
human DNA/

Pre-produced unique datamatrix code on tube,
datamatrix code and linear barcode on rack/

Height-reduced screw cap conserving up to 30 %freezer space/

Customised code sequences on request(order form F071003)/

SterileFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Height: 18.7 mm, Total rack height: 21.6 mm, Ø: 8.8 mm, Barcode type: datamatrix code, Raw material: PP,
Working volume: ≤235 µl, Cap design: screw cap, Sterile: +Item no.

BarcodeCap colour

Type of packaging

SterileQty. inner / outer976561yes

l naturalinternalrack+

480 / 960

976566yesl greeninternalrack+

480 / 960

976565yes

l yellowinternalrack+

480 / 960

976564yesl blueinternalrack+

480 / 960

976563yesl redinternalrack+

480 / 960

976568yes

l pinkinternalrack+

480 / 960

976569yes

l browninternalrack+

480 / 960

976567yesl blackinternalrack+

480 / 960

131263yes

l naturalinternalbulk+

480 / 960

/ Follow the instructions of use provided within each box.
/ USP class VI certified medical grade polypropylene

206 /Thread type

www.gbo.com

Cryotechnics
Cryo.s Biobanking Tubes

Cryo.s Biobanking Tubes
300 µlFREE OFdetectable
DNase

IVD

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Pre-produced unique datamatrix code on tube,
datamatrix code and linear barcode on rack/

Height-reduced screw cap conserving up to 30 %freezer space/

Customised code sequences on request(order form F071003)

noncytotoxic

nonpyrogenic

Height: 18.7 mm, Total rack height: 21.6 mm, Ø: 8.8 mm, Barcode type: datamatrix code, Raw material: PP, Working
volume: ≤235 µl

Item no.

BarcodeCap colour

Cap design

Thread typeType of packaging

Qty. inner / outer

without screw cap

internalrack

480 / 960

976570yes

976580yes

l natural

screw capinternalrack

480 / 960

976586yesl greenscrew capinternalrack

480 / 960

976585yes

l yellowscrew capinternalrack

480 / 960

976584yesl bluescrew capinternalrack

480 / 960

976583yes

l red

screw capinternalrack

480 / 960

976588yes

l pinkscrew capinternalrack

480 / 960

976589yes

l brownscrew capinternalrack

480 / 960

976587yesl blackscrew capinternalrack

480 / 960

131202yes

l natural

screw capinternalbulk

480 / 960

/ Follow the instructions of use provided within each box.
/ USP class VI certified medical grade polypropylene

www.gbo.com

/ 207

Cryotechnics
Cryo.s Biobanking Tubes

Cryo.s Biobanking Tubes sterile
600 µlFREE OFdetectable
DNase

STERILE

IVD

0197

FREE OFdetectable
human DNA/

Pre-produced unique datamatrix code on tube,
datamatrix code and linear barcode on rack/

Height-reduced screw cap conserving up to 30 %freezer space/

Customised code sequences on request(order form F071003)/

SterileFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Height: 33.3 mm, Total rack height: 36.2 mm, Ø: 8.8 mm, Barcode type: datamatrix code, Raw material: PP,
Working volume: ≤580 µl, Cap design: screw cap, Sterile: +Item no.

BarcodeCap colour

Type of packaging

SterileQty. inner / outer977561yes

l naturalinternalrack+

192 / 960

977566yesl greeninternalrack+

192 / 960

977565yes

l yellowinternalrack+

192 / 960

977564yesl blueinternalrack+

192 / 960

977563yesl redinternalrack+

192 / 960

977568yes

l pinkinternalrack+

192 / 960

977569yes

l browninternalrack+

192 / 960

977567yesl blackinternalrack+

192 / 960

132263yes

l naturalinternalbulk+

192 / 960

/ Follow the instructions of use provided within each box.
/ USP class VI certified medical grade polypropylene

208 /Thread type

www.gbo.com

Cryotechnics
Cryo.s Biobanking Tubes

Cryo.s Biobanking Tubes
600 µlFREE OFdetectable
DNase

IVD

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Pre-produced unique datamatrix code on tube,
datamatrix code and linear barcode on rack/

Height-reduced screw cap conserving up to 30 %freezer space/

Customised code sequences on request(order form F071003)

noncytotoxic

nonpyrogenic

Height: 33.3 mm, Total rack height: 36.2 mm, Ø: 8.8 mm, Barcode type: datamatrix code, Raw material: PP,
Working volume: ≤580 µl

Item no.

BarcodeCap colour

Cap design

Thread typeType of packaging

Qty. inner / outer

without screw cap

internalrack

192 / 960

977570yes

977580yes

l natural

screw capinternalrack

192 / 960

977586yesl greenscrew capinternalrack

192 / 960

977585yes

l yellowscrew capinternalrack

192 / 960

977584yesl bluescrew capinternalrack

192 / 960

977583yes

l red

screw capinternalrack

192 / 960

977588yes

l pinkscrew capinternalrack

192 / 960

977589yes

l brownscrew capinternalrack

192 / 960

977587yesl blackscrew capinternalrack

192 / 960

132202yes

l natural

screw capinternalbulk

192 / 960

/ Follow the instructions of use provided within each box.
/ USP class VI certified medical grade polypropylene

www.gbo.com

/ 209

Cryotechnics
Cryo.s Biobanking Tubes

Cryo.s Biobanking Tubes sterile
1000 µl

FREE OFdetectable
DNase

STERILE

IVD

0197

FREE OFdetectable
human DNA/

Pre-produced unique datamatrix code on tube,
datamatrix code and linear barcode on rack/

Height-reduced screw cap conserving up to 30 %freezer space/

Customised code sequences on request(order form F071003)/

USP class VI certified medical grade polypropylene/

SterileFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic

Height: 50.8 mm, Total rack height: 53.9 mm, Ø: 8.8 mm, Barcode type: datamatrix code, Raw material: PP,
Working volume: ≤975 µl, Cap design: screw cap, Sterile: +Item no.

BarcodeCap colour

Type of packaging

SterileQty. inner / outer

978561yes

l naturalinternalrack+

192 / 960

978566yesl greeninternalrack+

192 / 960

978565yes

l yellowinternalrack+

192 / 960

978564yesl blueinternalrack+

192 / 960

978563yesl redinternalrack+

192 / 960

978568yes

l pinkinternalrack+

192 / 960

978569yes

l browninternalrack+

192 / 960

978567yesl blackinternalrack+

192 / 960

133263yes

l naturalinternalbulk+

192 / 960

/ Follow the instructions of use provided within each box.
/ USP class VI certified medical grade polypropylene

210 /Thread type

www.gbo.com

Cryotechnics
Cryo.s Biobanking Tubes

Cryo.s Biobanking Tubes
1000 µl

FREE OFdetectable
DNase

IVD

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Pre-produced unique datamatrix code on tube,
datamatrix code and linear barcode on rack/

Height-reduced screw cap conserving up to 30 %freezer space/

Customised code sequences on request(order form F071003)/

USP class VI certified medical grade polypropylene

noncytotoxic

nonpyrogenic

Height: 50.8 mm, Total rack height: 53.9 mm, Ø: 8.8 mm, Barcode type: datamatrix code, Raw material: PP,
Working volume: ≤975 µl

Item no.

BarcodeCap colour

Cap design

Thread typeType of packaging

Qty. inner / outer

without screw cap

internalrack

192 / 960

978570yes

978580yes

l natural

screw capinternalrack

192 / 960

978586yesl greenscrew capinternalrack

192 / 960

978585yes

l yellowscrew capinternalrack

192 / 960

978584yesl bluescrew capinternalrack

192 / 960

978583yes

l red

screw capinternalrack

192 / 960

978588yes

l pinkscrew capinternalrack

192 / 960

978589yes

l brownscrew capinternalrack

192 / 960

978587yesl blackscrew capinternalrack

192 / 960

133202yes

l natural

screw capinternalbulk

192 / 960

/ Follow the instructions of use provided within each box.
/ USP class VI certified medical grade polypropylene

www.gbo.com

/ 211

Cryotechnics
Cryo.s Biobanking Tubes

Screw Caps
Biobanking Tubes

IVD

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

96 screw caps in cap carrier/

Non-sterile

noncytotoxic

nonpyrogenic

Description: 96 screw caps in cap carrier, Raw material: PP, Cap design: screw cap

Item no.

BarcodeCap colour

Qty. inner / outer

385270nol natural

960 / 960

385276nol green960 / 960

385275nol yellow

960 / 960

385274nol blue960 / 960

385273nol red

960 / 960

385278nol pink

960 / 960

385279nol brown

960 / 960

385277nol black

960 / 960

96-way Datamatrix Cryo Rack

IVD

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Pre-produced unique datamatrix code ECC 200 andlinear barcode type 128/

Made from polycarbonate

noncytotoxic

nonpyrogenic

Barcode type: linear barcode and datamatrix code, Raw material: PC

Item no.

212 /HeightBarcode976501

19.1 mmyes

97750133.7 mmyes

www.gbo.com

Rack colour

Suitable for Cryo.s

Qty. inner / outerl black

300 μl5 / 10l black

600 μl2 / 10Cryotechnics
Cryo.s Biobanking Tubes

Item no.

978501HeightBarcode51.4 mmyes

Rack colour

Suitable for Cryo.s

Qty. inner / outer

1000 μl

2 / 10l black

Cryo.s Decapper
The new Cryo.s Decapper enables the precise
simultaneous de- and recapping of cryotubes.
/

Individual motors for precise application of defined
torque values/

6 channel version for 1, 2 and 4 ml Cryo.s in 48 way
cryo racks/

8 channel version for Cryo.s Biobanking Tubes/

Docking station

NEW

Content kit: 1 Decapper, Power supply unit, Docking station with charging function, Quick start guide
Item no.

Description

Plug

Qty. inner / outer

852076-EU

6-channel decapper

Europe- /1852076-UK

6-channel decapper

UK

- /1

852076-NA

6-channel decapper

US

- /1

852076-CN

6-channel decapper

China

- /1

852076-AU

6-channel decapper

Australia / NZ

- /1852076-JP

6-channel decapper

Japan

- /1

852078-EU

8-channel decapper

Europe- /1852078-UK

8-channel decapper

UK

- /1

852078-NA

8-channel decapper

US

- /1

852078-CN

8-channel decapper

China

- /1

852078-AU

8-channel decapper

Australia / NZ

- /1852078-JP

8-channel decapper

Japan

- /1

www.gbo.com

/ 213

Cryotechnics
Cryo.s Biobanking Tubes

Cryo.s Rack ScannerWith the Cryo.s rack scanner, Greiner Bio-One
provides an optimum solution for scanning
Cryo.s with datamatrix and Cryo.s biobanking
tubes in SLAS-standard formatted racks.
/

Single tube scan feature/

Small footprint/

5 year warranty

Height: 7 cm, Length: 31 cm, Width: 19.5 cm, Content kit: 1 scanner, power cable, USB cable, driver, user manual

Item no.

214 /Description

Plug

Qty. inner / outer

849070rack scanner EU/UK/Asia edition

Europe1/1849050rack scanner US edition

US

1/1

www.gbo.com

Cryotechnics
Cryo.s Biobanking Tubes

www.gbo.com

/ 215

For the handling and analysis of chemical and biological samples
with small or medium volumes, Greiner Bio-One offers a wide rangeof different analyser cups and reaction vessels with corresponding
closures and cuvettes.

216 /www.gbo.com

Reaction Tubes / Analyser Cups
﻿

REACTION TUBES / ANALYSER CUPS
/ Reaction Tubes Analyser Cups������������ 218
Reaction Tubes ��������������������������������������������� 219
Reaction Tubes 5 ml��������������������������������������� 219
Closures for Reaction Tubes �������������������������220
Analyser Cups �����������������������������������������������220/ Semi-micro / Macro Cuvette ��������������� 221
Semi-micro / Macro Cuvette �������������������������222

www.gbo.com

/ 217

Reaction Tubes / Analyser Cups
Reaction Tubes Analyser Cups/

For the max. relative
centrifugal force (RCF),
please refer to thetechnical appendix.

/

For volumes from 0.5 ml
up to 5 ml/

With or without cap/

With or without graduation

REACTION TUBESANALYSER CUPS
Reaction tubes from Greiner
Bio-One are available in manydifferent versions. They have ahigh chemical and temperature resistance and are available
from 0.5 ml to 5 ml for small andmedium volumes. The reaction
tubes are made of high-quality
polypropylene and are available
with or without lid. The lids ofthe corresponding vessels areflat and frosted to ensure easylabelling.
Brown reaction tubes have been
specially developed for working with light-sensitive sample material. For working withliquid volumes from 1 to 5 ml,
Greiner Bio-One offers 5 ml reaction tubes with screw cap or

218 /www.gbo.com

snap cap. The tubes are also
made of highly transparent polypropylene and allow contamination-free access when pipetting
medium sample volumes. They
also have a frosted, writable surface. This ensures reliable sample identification in everyday laboratory work.
Analyser cups are available withconical or flat bottom design andwith or without cap. All analyser cups are manufactured ofhighly transparent polystyrene.
The volumes range from 1.7 ml
up to 25 ml. We offer analyser
cups that are suitable for the
analytical systems of Hitachi
or Coulter/Hycel.

Reaction Tubes / Analyser Cups
Reaction Tubes Analyser Cups

Reaction Tubes

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

High chemical resistance and temperature tolerance/

Flat, frosted lids and caps for easy labelling/

Brown tube for light-sensitive materials

nonpyrogenic

Description: reaction tube
Item no.

FeatureSupport skirt

Graduation

Product colour

Volume range

Caps
attached

Cap design

Qty. inner / outer

667201

Vitatron-l natural≤0.5 mlyes

snap cap

1.000 / 10.000

616201

universal

yes

l natural≤1.5 mlyes

snap cap

500 / 4.000

616283

universal

yes

l brown≤1.5 mlyes

snap cap

500 / 4.000

623201

universal

yes

l natural

≤2 ml

yes

snap cap

500 / 4.000

716201noyes

l natural≤1.5 mlscrew cap

500 / 5.000

717201yes

yes

l natural≤1.5 mlscrew cap

500 / 5.000

722201yes

yes

l natural≤2 mlscrew cap

500 / 5.000/ Coloured versions of item no. 616201 and 623201 as well as sterile versions of all tubes are available on request.

Reaction Tubes
5 ml
5 ml reaction tubes with either a snap cap forfast opening and closing or a screw cap for
secure sample storage.

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Outer diameter and shape similar to 15 ml tube/

Resealable packaging

nonpyrogenic

Description: reaction tube, Support skirt: no, Graduation: yes, Raw material: PP

Item no.

Product colour

Cap colour

Caps attached

Cap design

725201l naturall bluescrew cap

725261l naturall bluescrew cap

SterileQty. inner / outer

100 / 500+

50 / 500

www.gbo.com

/ 219

Reaction Tubes / Analyser Cups
Reaction Tubes Analyser Cups

Item no.

Product colour

Cap colour

Caps attached

Cap design

622201l naturalyessnap cap

622261l naturalyessnap cap

SterileQty. inner / outer

100 / 1.000+

100 / 1.000

Closures for Reaction Tubes

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

/

Suitable for item no. 716XXX, 717XXX and 722XXX/

Available in natural, red, blue, green and yellow/

With gasket

nonpyrogenic

Description: screw cap, Feature: with sealing ring, Suitable for tubes Ø: 12 mm, Cap design: screw cap
Item no.

Product colour

Qty. inner / outer366380l natural

500 / 5.000

366383l red

500 / 5.000

366384l blue500 / 5.000

366385l green

500 / 5.000

366386l yellow

500 / 5.000

Analyser Cups/

For handling and analysis of chemical and biological samples/

Available for different analytical systems

Description: analyser cup, Raw material: PS, Caps attached: no
Item no.

220 /FeatureSupport skirt

Bottom shape

Volume range

Lid type

Qty. inner / outer

668102

Coulter / Hycel

no

flat

≤25 mlwith lid

250 / 1.250

729101

Hitachiyesconical≤1.7 mlwithout lid

250 / 5.000

www.gbo.com

Reaction Tubes / Analyser Cups
Semi-micro / Macro Cuvette/

For further information, please refer to our
website: www.gbo.com

SEMI-MICRO / MACRO CUVETTE/

Available as semi-micro or macro cuvettes/

Very thin walls for fastand even temperature
control/

Low light scatteringand high transmission
rate/

Suitable for a wavelength range from 340900 nm

Cuvettes are used for UV/VIS
spectroscopy. Greiner Bio-One
offers semi-micro cuvettes witha total volume of 1.6 ml and macro
cuvettes with a total volume of
4 ml. Both versions are made of
crystal-clear polystyrene andare particularly suitable for enzymatic determinations, as thevery thin wall thickness enables
fast and even temperature control. Our cuvettes are characterised by their low light scattering combined with a hightransmission rate. Semi-micro
and macro cuvettes can be used
in a wavelength range from 340
nm to 900 nm.

www.gbo.com

/ 221

Reaction Tubes / Analyser Cups
Semi-micro / Macro Cuvette

Semi-micro / Macro Cuvette/

Ideal for enzymatic determinations/

Manufactured from crystal clear polystyrene/

Minimal light scatter but high transmission rate/

Applicable wavelength from 340 to 900 nm

Height: 45 mm, Length: 12.5 mm, Width: 12.5 mm, Pathlength: 10 mm, Raw material: PS

Item no.

Description

Working volume

Total volume

Qty. inner / outer

613101semi-micro cuvette≥0.95 ml

1.6 ml100 / 1.000

614101

macro cuvette≥2.5 ml

4 ml

100 / 1.000/ Cuvettes

222 /www.gbo.com

Reaction Tubes / Analyser Cups
Semi-micro / Macro Cuvettewww.gbo.com

/ 223

PCR (Polymerase Chain Reaction) is an important molecularbiological method to determine processes in living organisms at themolecular level or genetic changes in the genetic material. It is used
for the detection of diseases and parentage relationships as well as
in forensics.

224 /www.gbo.com

Molecular Biology
﻿

MOLECULAR BIOLOGY/ Sapphire PCR Tubes��������������������������� 226
PCR Tubes �����������������������������������������������������227
PCR 8-Tube Strips �����������������������������������������228
Sapphire PCR 8-Cap Strips ���������������������������229/ Sapphire PCR Microplates������������������ 230
96 Well PCR Microplates �������������������������������� 231
384 Well PCR Microplates ������������������������������ 231

www.gbo.com

/ 225

Molecular Biology
Sapphire PCR Tubes/

For standard reaction
tubes please refer tochapter reaction tubes/ analyser cups.

/

Manufactured out of
medical-grade
polypropylene without
interfering additives/

Thin-walled for
efficient heat transfer
during amplification/

Comprehensive thermal cycler
compatibility

SAPPHIRE
PCR TUBESThe Sapphire PCR tube portfolio comprises a full range of
PCR reaction tubes (0.2 ml and
0.5 ml) as well as PCR 8-tube
strips and the matching 8-cap
strips.
The thin wall construction optimises the heat transfer from theblock to the reaction solution.
PCR tubes with flat caps are suitable for real-time / quantitative
PCR and have frosted caps foreasy labelling.
The classical Sapphire PCR
8-tube strips are also available

226 /www.gbo.com

with individually attached caps.
The angular attached domed orflat caps can be closed and opened individually minimising therisk of cross contamination. Moreover, individual closing andopening helps to avoid pipetting errors and facilitates sample processing.
PCR 8-tube strips are also available as low profile tubes witha well volume of 0.1 ml ideal for
FAST, standard and real-time
PCR supporting the reductionof cycle time without compromises in accuracy and efficiency.

Molecular Biology
Sapphire PCR Tubes

PCR Tubes
Tubes with flat caps are suitable for realtime/quantitative PCR and have frosted caps foreasy labelling.
/

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

nonpyrogenic

Packaging labelled with product symbols

PCR

Description: PCR tube, Raw material: PP
Item no.

Product colour

VolumeVolume range

Caps attached

Cap design

Lid type

Qty. inner / outer

671201l natural

0.2 ml≤0.2 mlyes

individuallyattached

domed

1.000 / 10.000

671273l red

0.2 ml≤0.2 mlyes

individuallyattached

domed

1.000 / 10.000

671274l blue0.2 ml≤0.2 mlyes

individuallyattached

domed

1.000 / 10.000

671275l green

0.2 ml≤0.2 mlyes

individuallyattached

domed

1.000 / 10.000

671276l yellow

0.2 ml≤0.2 mlyes

individuallyattached

domed

1.000 / 10.000

671277l violet

0.2 ml≤0.2 mlyes

individuallyattached

domed

1.000 / 10.000

671281assorted

0.2 ml≤0.2 mlyes

individuallyattached

domed

1.000 / 10.000

683201l natural

0.2 ml≤0.2 mlyes

individuallyattached

flat

1.000 / 10.000

683273l red

0.2 ml≤0.2 mlyes

individuallyattached

flat

1.000 / 10.000

683274l blue0.2 ml≤0.2 mlyes

individuallyattached

flat

1.000 / 10.000

683275l green

0.2 ml≤0.2 mlyes

individuallyattached

flat

1.000 / 10.000

683276l yellow

0.2 ml≤0.2 mlyes

individuallyattached

flat

1.000 / 10.000

683277l violet

0.2 ml≤0.2 mlyes

individuallyattached

flat

1.000 / 10.000

683271assorted

0.2 ml≤0.2 mlyes

individuallyattached

flat

1.000 / 10.000

682201l natural

0.5 ml≤0.5 mlyes

individuallyattached

flat

1.000 / 10.000

682273l red

0.5 ml≤0.5 mlyes

individuallyattached

flat

1.000 / 10.000

682274l blue

0.5 ml≤0.5 mlyes

individuallyattached

flat

1.000 / 10.000

www.gbo.com

/ 227

Molecular Biology
Sapphire PCR Tubes

Item no.

Product colour

VolumeVolume range

Caps attached

Cap design

Lid type

Qty. inner / outer

682275l green

0.5 ml≤0.5 mlyes

individuallyattached

flat

1.000 / 10.000

682276l yellow

0.5 ml≤0.5 mlyes

individuallyattached

flat

1.000 / 10.000

682277l violet

0.5 ml≤0.5 mlyes

individuallyattached

flat

1.000 / 10.000

682281assorted

0.5 ml≤0.5 mlyes

individuallyattached

flat

1.000 / 10.000

684201l natural

0.2 ml≤0.2 mlno

without lid

1.000 / 10.000/ Assorted colour contains the following colours: red, blue, green, yellow, violet

PCR 8-Tube Strips

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

nonpyrogenic/

Thin wall for optimal heat transfer/

Low evaporation rate in PCR/

Also available with individually attached caps or as
low-profile version/

Packaging labelled with product symbols

PCR

Description: PCR 8-Tube Strips, Raw material: PP
Item no.

Product colour

Volume range

Caps attached

Cap design

673210l natural≤0.2 mlnoseparate cap strip

125 / 1.250

673273l red≤0.2 mlnoseparate cap strip

125 / 1.250

673274l blue≤0.2 mlnoseparate cap strip

125 / 1.250

673275l green≤0.2 mlnoseparate cap strip

125 / 1.250

673276l yellow≤0.2 mlnoseparate cap strip

125 / 1.250

673277l violet≤0.2 mlnoseparate cap strip

125 / 1.250

673271assorted≤0.2 mlnoseparate cap strip

125 / 1.250

l natural≤0.1 mlyes

individually attachedflat

120 / 1.200

l natural≤0.2 mlyes

individually attachedflat

120 / 1.200

671221Featurelow profile

608281

Qty. inner / outer673281

low profilel natural≤0.1 mlnoseparate cap strip

flat

125 / 1.250

673283breakablel natural≤0.2 mlyes

individually attached

domed

120 / 1.200/ Assorted colour contains the following colours: red, blue, green, yellow, violet

228 /Lid type

www.gbo.com

Molecular Biology
Sapphire PCR Tubes

Sapphire PCR 8-Cap Strips

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

nonpyrogenic/

Real-time PCR cap strips (Item No. 373250) have aflat lid and are made of highly transparent
polypropylene/

These are ideal for real-time PCR applications andare compatible with most real-time PCR devices

PCR

Description: 8-cap strips, Raw material: PP
Item no.

FeatureProduct colour

Qty. inner / outer

373270l natural

125 / 1.250

373273l red

125 / 1.250

373274l blue125 / 1.250

373275l green

125 / 1.250

373276l yellow

125 / 1.250

373277l violet

125 / 1.250

373281assorted

125 / 1.250

l natural

125 / 1.250

373250for RT PCR/ Assorted colour contains the following colours: red, blue, green, yellow, violet

www.gbo.com

/ 229

Molecular Biology
Sapphire PCR Microplates/

For details regarding
plate geometry as wellas compability withthermal cyclers, pleaserefer to the technicalappendix.

/Available in 96 well and
384 well format/

Ultra-thinpolypropylene for optimal heat transfer/

Sealable with sealers
SILVERseal, VIEWseal
and AMPLIseal

SAPPHIRE
PCR MICROPLATESThe use of the 96 well format
allows the scale up of basic
PCR work, while the 384 wellformat is ideal for high-throughput screening projects.
All microplates are made of thinwalled polypropylene. This op-

230 /www.gbo.com

timises the heat transfer fromthe thermoblock to the reaction
solution. Our heat-resistantsealers AMPLIseal, VIEWseal
and SILVERseal are ideal for sealing the microplates during PCR,
and the 96 well microplate may
also be sealed with 8-cap strips.

Molecular Biology
Sapphire PCR Microplates

96 Well PCR Microplates

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

nonpyrogenic/

Without skirt, half-skirt and full-skirt/

Alphanumeric printing for rapid sample
identification/

Sealable with sealers SILVERseal , VIEWseal and
AMPLIseal or with compatible 8-cap strips/

Low profile microplate for reduced sample volume
and cycle time

PCR

Well format: 96, Raw material: PP
Item no.

Skirt

Product colour

Colour alphanumeric code

without skirt

l natural

Feature652201

Well volume, max

Qty. inner / outerl black

0.2 ml10 / 100

652250flat, universal

without skirt

l naturall black

0.2 ml10 / 100

652210low profile

without skirt

l naturall black

0.1 ml10 / 100669285

suitable for LightCycler®

half-skirt

l whitel black

0.2 ml10 / 100652290suitable for ABI

half-skirt

l naturall black

0.2 ml10 / 100652260

ABI design

half-skirt

l naturall black

0.2 ml10 / 100yes

l naturall black

0.2 ml10 / 100

652270384 Well PCR Microplates

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

nonpyrogenic/

Thin wall for optimal heat transfer/

Alphanumeric well coding/

Sealable with sealers SILVERseal , VIEWseal and
AMPLIseal/

White and black microplate versions are available
on request

PCR

Well format: 384, Skirt: yes, Raw material: PP, Total volume (well): 25 µl

Item no.

FeatureProduct colour

Colour alphanumeric code

Qty. inner / outer785290suitable for ABI

l naturall blue15 / 60785285

suitable for LightCycler®

l whitel black

50 / 100

www.gbo.com

/ 231

To achieve consistent, reproducible results when handling liquids
in the laboratory, reliable dispensers and pipette tips are essential.
With high quality materials and pipetting solutions, maximum
precision can be achieved while reducing the consumption ofreagents and time. Comfortable and fatigue-free working is also
crucial for everyday laboratory life.

232 /www.gbo.com

Liquid Handling﻿

LIQUID HANDLING/ Sapphire Pipettes������������������������������ 234
Single-Channel Pipettes �������������������������������235
Multi-Channel Pipettes ���������������������������������235
Pipette Carrousel ������������������������������������������236/ Sapphire Pipette Tips�������������������������237
Pipette Tips 10 µl�������������������������������������������238
Pipette Tips 10 µl extended����������������������������239
Filter Tips 20 µl����������������������������������������������240
Filter Tips 100 µl��������������������������������������������240
Pipette Tips 200 µl������������������������������������������241
Pipette Tips 300 µl�����������������������������������������242
Pipette Tips 1000 µl���������������������������������������243
Pipette Tips 1250 µl����������������������������������������243
Racks (unfilled)����������������������������������������������244
Macro Pipette Tip 1-5 ml��������������������������������245
Gel-Load Pipette Tips 1-200 µl�����������������������245/ CELLSTAR® Serological Pipettes������� 246
Serological Pipettes ������������������������������������� 247
Serological Pipettes Triple-packed���������������248
Serological Pipettes Special Models��������������248
MaxiPette �����������������������������������������������������249
Pasteur / Serum Pipettes ������������������������������249

www.gbo.com

/ 233

Liquid Handling
Sapphire Pipettes/

Watch our video:/

Outstanding accuracy
and precision/

Ergonomic design
and minimal pipetting
forces for a new level ofcomfort/Lightweight, robust
and fully autoclavable/

Digital volume setting/

Colour-coded for tip
identification

SAPPHIRE
PIPETTES
Sapphire pipettes are a variable volume range of air-displacement pipettes designed
and built to deliver optimal
performance, ergonomics, androbustness in day-to-day use.
The lightweight design coupledwith a state-of-the-art mechanism reduces pipetting forces
and makes our pipettes both
comfortable and easy-to-use.
The contoured body shape allows for a relaxed grip whateversize your hand, and the 3-position tip ejector button makes

234 /www.gbo.com

pipetting comfortable for bothleft-handed and right-handed
users. The colour-coded pushbutton allows for easier identification of the relevant tips.
Sapphire pipettes provide an
excellent performance within ISO 8655 which is considered the basis for determining
the accuracy and precision of
air-displacement pipettes. In
combination with Sapphire pipette tips they show one of thelowest error tolerances on themarket. All Sapphire pipettes are
fully autoclavable.

Liquid Handling
Sapphire Pipettes

Single-Channel Pipettes/

Light and comfortable design/

Low pipetting forces for ease of use/

Digital volume setting/

Colour-coded for tip identification/

5 ml & 10 ml pipettes without ejector lever/

Fully autoclavable

Description: single-channel pipette
Item no.

Colour code

Volume range

Qty. inner / outer

89000002

l orange

0.2 - 2 µl

1/1

89000010

l red

1 - 10 µl

1/1

89000020

l light yellow

2 - 20 µl

1/1

89000100l light orange color_circle

10 - 100 µl

1/1

89000200l yellow

20 - 200 µl

1/1

89001000

l blue100 - 1,000 µl

1/1

89000500

l violet

500 - 5,000 µl

1/1

89010000

l light blue1000 - 10.000 µl

1/1/ Spare parts and other accessories available on request

Multi-Channel Pipettes

Item no.

Description

89000810

8-channel pipette

890008208-channel pipette/

Light and comfortable design/

Low pipetting forces for ease of use/

Digital volume setting/

Colour-coded for tip identification/

Optimised for use with Greiner Bio-One tips/

Fully autoclavable

Colour code

Volume range

Qty. inner / outerl red

0.5 - 10 µl

1/1

l light yellow

2 - 20 µl

1/1

www.gbo.com

/ 235

Liquid Handling
Sapphire Pipettes

Item no.

Description

890082008-channel pipette

89008300

Colour code

Volume range

Qty. inner / outerl yellow

20 - 200 µl

1/1

8-channel pipettel green20 - 300 µl

1/1

8900121012-channel pipettel red

0.5 - 10 µl

1/1

8900122012-channel pipettel light yellow

2 - 20 µl

1/1

89012200

12-channel pipettel yellow

20 - 200 µl

1/1

89012300

12-channel pipettel green20 - 300 µl

1/1/ Spare parts and other accessories available on request

Pipette Carrousel

236 /

/

Holds up to 7 pipettes/

Robust and space-saving/

Holds single and multi-channel pipettes

Item no.

Qty. inner / outer

89000099

1/1

www.gbo.com

Liquid Handling
Sapphire Pipette Tips/

Watch our video:

https://www.youtube.com/
watch?v=VMbTjs4sSVY

SAPPHIRE
PIPETTE TIPS/

Fully automated production and packaging/

Universal fit for allcommon pipettes/

Tips (without filter) andracks are autoclavable/

Coloured box inserts
for easy volume identification/

Low retention surface
properties for highrecovery rate andmaximum precision

We understand that flexibility is critical in terms of modern liquid handling solutions.
This is why the Sapphire tips
are manufactured in eight different sizes in a volume range
from 10 µl to 1250 µl, including
an extended length 10 µl tip forthe recovery of small sample
volumes.
All our tips are made of medi-

cal grade polypropylene and areavailable as standard, low-retention, filter or low-retention
filter tips.
Depending on the format,
Sapphire pipette tips are packed in racks, bulk or refill units.
Sapphire tips and pipettes whenused together provide a fully optimised and harmonised liquidhandling solution.

www.gbo.com

/ 237

Liquid Handling
Sapphire Pipette Tips

Pipette Tips
10 µl

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic/

Universal fit for all common pipettes/

Tips (without filter) and racks are autoclavable/

Easy-to-read graduation/

Colour-coded for tip identification

PCR

Raw material: PP, Volume range: 0.2 - 10 µl, Nominal volume: 10 µl, Matching rack: 770310

Item no.

238 /FeatureProduct colour

Box insert colour

Type of packaging

SterileQty. inner / outer

771350standard

l natural

l red

bag

1.000 / 10.000

771351standard

l natural

l red

rack

960 / 5.760

771352standard

l natural

l red

rack

771354standard

l naturall redrefill771355standard

l naturall redrefill771361

low retention

l natural

l red

rack

771362

low retention

l natural

l red

rack

771364

low retention

l naturall redrefill771365

low retention

l naturall redrefill+960 / 4.800

771353filterl natural

l red

rack

+

960 / 5.760

771363

low retention filter

l natural

l red

rack

+

960 / 5.760

www.gbo.com+

960 / 5.760
960 / 4.800+

960 / 4.800
960 / 5.760+

960 / 5.760
960 / 4.800

Liquid Handling
Sapphire Pipette Tips

Pipette Tips
10 µl extended

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic/

Extended 10 µl tip for better recovery of small
sample volumes/

Universal fit for all common pipettes/

Tips (without filter) and racks are autoclavable/

Easy-to-read graduation

PCR

Raw material: PP, Volume range: 0.5 - 10 µl, Nominal volume: 10 µl, Matching rack: 770310

Item no.

FeatureProduct colour

Box insert colour

Type of packaging

SterileQty. inner / outer

772350standard

l natural

l red

bag

1.000 / 5.000

772351standard

l natural

l red

rack

960 / 5.760

772352standard

l natural

l red

rack

772354standard

l naturall redrefill772355standard

l naturall redrefill772361

low retention

l natural

l red

rack

772362

low retention

l natural

l red

rack

772364

low retention

l naturall redrefill772365

low retention

l naturall redrefill+960 / 4.800

772353filterl natural

l red

rack

+

960 / 5.760

772363

low retention filter

l natural

l red

rack

+

960 / 5.760+

960 / 5.760
960 / 4.800+

960 / 4.800
960 / 5.760+

960 / 5.760
960 / 4.800

www.gbo.com

/ 239

Liquid Handling
Sapphire Pipette Tips

Filter Tips
20 µl

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Prevent contamination with liquids and aerosols
during pipetting/

filters manufactured without additives/

Easy-to-read graduation/

Colour-coded for tip identification

nonpyrogenic

PCR

Raw material: PP, Volume range: 2 - 20 µl, Nominal volume: 20 µl, Type of packaging: rack, Sterile: +Item no.

FeatureProduct colour

Box insert colour

SterileQty. inner / outer

773353filterl naturall orange+

960 / 5.760

773363

low retention filter

l naturall orange+

960 / 5.760

Filter Tips
100 µlSTERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Prevent contamination with liquids and aerosols
during pipetting/

filters manufactured without additives/

Easy-to-read graduation/

Colour-coded for tip identification

nonpyrogenic

PCR

Raw material: PP, Volume range: 5 - 100 µl, Nominal volume: 100 µl, Type of packaging: rack, Sterile: +Item no.

240 /FeatureProduct colour

Box insert colour

SterileQty. inner / outer

774353filterl naturall dark yellow+

960 / 5.760

774363

low retention filter

l naturall dark yellow+

960 / 5.760

www.gbo.com

Liquid Handling
Sapphire Pipette Tips

Pipette Tips
200 µlFREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Universal fit for all common pipettes/

Tips (without filter) and racks are autoclavable/

Easy-to-read graduation/

Colour-coded for tip identification

nonpyrogenic

PCR

Raw material: PP, Volume range: 5 - 200 µl, Nominal volume: 200 µl, Matching rack: 770320

Item no.

FeatureProduct colour

Box insert colour

Type of packaging

SterileQty. inner / outer

775350standard

l naturall yellowbag

1.000 / 15.000

775351standard

l naturall yellowrack

960 / 5.760

775352standard

l naturall yellowrack775354standard

l naturall yellow

refill775355standard

l naturall yellow

refill775361

low retention

l naturall yellowrack775362

low retention

l naturall yellowrack775364

low retention

l naturall yellow

refill775365

low retention

l naturall yellow

refill+960 / 4.800

775353filterl naturall yellowrack+

960 / 5.760

775363

low retention filter

l naturall yellowrack+

960 / 5.760+

960 / 5.760
960 / 4.800+

960 / 4.800
960 / 5.760+

960 / 5.760
960 / 4.800

www.gbo.com

/ 241

Liquid Handling
Sapphire Pipette Tips

Pipette Tips
300 µlFREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Universal fit for all common pipettes/

Tips (without filter) and racks are autoclavable/

Easy-to-read graduation/

Colour-coded for tip identification

nonpyrogenic

PCR

Raw material: PP, Volume range: 10 - 300 µl, Nominal volume: 300 µl, Matching rack: 770330

Item no.

242 /FeatureProduct colour

Box insert colour

Type of packaging

SterileQty. inner / outer

776350standard

l naturall greenbag

1.000 / 10.000

776351standard

l naturall greenrack

960 / 5.760

776352standard

l naturall greenrack776354standard

l naturall greenrefill776355standard

l naturall greenrefill776361

low retention

l naturall greenrack

776362

low retention

l naturall greenrack

776364

low retention

l naturall greenrefill776365

low retention

l naturall greenrefill+960 / 4.800

776353filterl naturall greenrack+

960 / 5.760

776363

low retention filter

l naturall greenrack+

960 / 5.760

www.gbo.com+

960 / 5.760
960 / 4.800+

960 / 4.800
960 / 5.760+

960 / 5.760
960 / 4.800

Liquid Handling
Sapphire Pipette Tips

Pipette Tips
1000 µl

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Universal fit for all common pipettes/

Tips (without filter) and racks are autoclavable/

Easy-to-read graduation/

Colour-coded for tip identification

nonpyrogenic

PCR

Raw material: PP, Volume range: 50 - 1.000 µl, Nominal volume: 1,000 µl, Matching rack: 770340

Item no.

FeatureProduct colour

Box insert colour

Type of packaging

SterileQty. inner / outer

777350standard

l naturall bluebag

1.000 / 5.000

777351standard

l naturall bluerack

960 / 3.840

777352standard

l naturall bluerack

777354standard

l naturall bluerefill777355standard

l naturall bluerefill777361

low retention

l naturall bluerack

777362

low retention

l naturall bluerack

777364

low retention

l naturall bluerefill777365

low retention

l naturall bluerefill+960 / 3.840
960 / 4.800+

960 / 4.800
960 / 3.840+

960 / 3.840
960 / 4.800+

960 / 4.800

Pipette Tips
1250 µl

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic

nonpyrogenic/

Universal fit for all common pipettes/

Tips (without filter) and racks are autoclavable/

Easy-to-read graduation/

Colour-coded for tip identification

PCR

Raw material: PP, Volume range: 50 - 1.250 µl, Nominal volume: 1,250 µl, Matching rack: 770340

Item no.

778350Featurestandard

Product colour

Box insert colour

l naturall blueType of packaging

bag

SterileQty. inner / outer

1.000 / 5.000

www.gbo.com

/ 243

Liquid Handling
Sapphire Pipette Tips

Item no.

FeatureProduct colour

Box insert colour

Type of packaging

SterileQty. inner / outer

778351standard

l naturall bluerack778352standard

l naturall bluerack778354standard

l naturall bluerefill778355standard

l naturall bluerefill778361

low retention

l naturall bluerack778362

low retention

l naturall bluerack778364

low retention

l naturall bluerefill778365

low retention

l naturall bluerefill+960 / 4.800

778353filterl naturall bluerack+

960 / 3.840

778363

low retention filter

l naturall bluerack+

960 / 3.840

960 / 3.840+

960 / 3.840
960 / 4.800+

960 / 4.800
960 / 3.840+

960 / 3.840
960 / 4.800

Racks(unfilled)

FREE OFdetectable
DNase

FREE OFdetectable
human DNA

Item no.

244 //Highly transparent and stable/

Can be autoclaved and reused up to 50 times/

Stackable and automation-friendly/

Colour-coded rack inserts for quick volume identification

FREE OFdetectable
RNase

Description

FeatureBox insert colour

Rack colour

Qty. inner / outerl red

l natural

10 / 60

770310suitable for 10 µl / 10 µl ext.

770320suitable for 200 µl

l yellowl natural

10 / 60770330suitable for 300 µl

l greenl natural

10 / 60770340suitable for 1000 µl / 1250 µll bluel natural

10 / 40770370suitable for 10 - 300 µl

without rack insert

l natural

10 / 60770380suitable for 1000 - 1250 µl

without rack insert

l natural

10 / 40

www.gbo.com

Liquid Handling
Sapphire Pipette Tips

Macro Pipette Tip
1-5 ml/Volume range from 1 - 5 ml/

Suitable for Gilson® P5000

Feature: Macro, Graduation: -, Volume range: 1 - 5 ml
Item no.

745290

Product colour

Qty. inner / outerl natural

250 / 2.500

Gel-Load Pipette Tips
1-200 µl

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

nonpyrogenic/

Made from high-quality polypropylene/

Universal fit for all common pipettes/

5 µl graduation for easy control of the pipetted
volume/Tip with 0.57 mm outer diameter suitable forloading gel pockets/

83 mm total length to reach even the bottom ofnarrow vessels

PCR

Volume: 200 µl, Volume range: 1 - 200 µl, Type of packaging: bag

Item no.

775390

Product colour
l natural

Qty. inner / outer

1.000 / 10.000

www.gbo.com

/ 245

Liquid Handling
CELLSTAR® Serological Pipettes/

Serological pipettes
are also available triple-packed.

/

Tip design guarantees
drop-free pipetting/

Maximum accuracy/

High optical clarity/

Clear, easy to read
graduation/

A coloured vertical
Schellbach stripe on
1, 2, 5, 10 and 25 ml
pipettes makes it
considerably easier toread off the volume/

Colour code according
to international standards

CELLSTAR®
SEROLOGICAL PIPETTESGreiner Bio-One offers a wide
range of different serologicalpipettes and transfer pipettes.
The volume capacity of the serological pipettes is increased bya negative graduation. The pipettes are provided with colour
codes according to international
standards. The use of high-grade polystyrene guarantees maximum clarity. Expiry date and
LOT number are printed on each246 /www.gbo.com

single packaging unit. All pipettes are supplied with a filter forprotection against the suction ofliquid into the pipetting device.
CELLSTAR® serological pipettes are available in three different packaging options: inplastic bulk packs as well as individually wrapped in plastic or
paper/plastic, both with peel-off
function and additional breakthrough function.

Liquid Handling
CELLSTAR® Serological Pipettes

Serological Pipettes

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Sterile/Increased volume range with negative graduations/

Lot number and expiry date on each bag/

Filter for protection against suction of liquids into
the pipetting device/

Various packaging options

nonpyrogenic

Raw material: PS, Sterile: +Item no.

Description

Graduation

Nominal volume604107

1 ml pipette

1/100

1 ml

604160

1 ml pipette

1/100

1 ml

604181

1 ml pipette

1/100

1 ml

7101072 ml pipette

1/100

2 ml

710160

2 ml pipette

1/100

2 ml

710180

2 ml pipette

1/100

2 ml

606107

5 ml pipette

1/10

5 ml

606160

5 ml pipette

1/10

5 ml

606180

5 ml pipette

1/10

5 ml

607107

10 ml pipette

1/10

10 ml

607160

10 ml pipette

1/10

10 ml

607180

10 ml pipette

1/10

10 ml

76010725 ml pipette

2/10

25 ml

760160

25 ml pipette

2/10

25 ml

760180

25 ml pipette

2/10

76816050 ml pipette

76818050 ml pipette

PackagingType of packaging

SterileQty. inner / outer

bulk

+

25 / 1.000

plastic / plastic

single-packed+

100 / 1.000

paper / plastic

single-packed+

100 / 1.000

bulk

+

25 / 1.000

plastic / plastic

single-packed+

100 / 1.000

paper / plastic

single-packed+

100 / 1.000

bulk

+

25 / 500

plastic / plastic

single-packed+

50 / 200

paper / plastic

single-packed+

50 / 200bulk+

25 / 500

plastic / plastic

single-packed+

50 / 200

paper / plastic

single-packed+

50 / 200bulk+

25 / 200

plastic / plastic

single-packed+

50 / 200

25 ml

paper / plastic

single-packed+

50 / 200

1/2

50 ml

plastic / plastic

single-packed+

20 / 100

1/2

50 ml

paper / plastic

single-packed+

20 / 100

www.gbo.com

/ 247

Liquid Handling
CELLSTAR® Serological Pipettes

Serological Pipettes
Triple-packed

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Sterile/Increased volume range with negative graduations/

Lot number and expiry date on each bag/

Filter for protection against suction of liquids into
the pipetting device/

Triple-packed

nonpyrogenic

Raw material: PS, Packaging: plastic / plastic, Triple-packed: yes, Sterile: +Item no.

Description

Graduation

Nominal volume

SterileQty. inner / outer

604160-TRI

1 ml pipette

1/100

1 ml

+

10 / 100

710160-TRI

2 ml pipette

1/100

2 ml

+

10 / 100

606160-TRI

5 ml pipette

1/10

5 ml

+

10 / 100

607160-TRI

10 ml pipette

1/10

10 ml

+

10 / 100760160-TRI

25 ml pipette

2/10

25 ml

+

10 / 100

768160-TRI

50 ml pipette

1/2

50 ml

+

10 / 100

Serological Pipettes
Special Models

STERILE

FREE OFdetectable
DNase

FREE OFdetectable
human DNAFREE OFdetectable
RNase

noncytotoxic/

Short-format pipettes (shorties) permit
back-saving work/

2 ml aspiration pipette without plug and graduation/

Single-packed

nonpyrogenic

Raw material: PS, Sterile: +Item no.

248 /Description

FeatureGraduation

Nominal volume

Packaging

SterileQty. inner / outer

710183

2 ml aspiration pipetteno plug-

2 ml

paper / plastic+

100 / 1.000

606190

5 ml pipetteshorty1/10

5 ml

plastic / plastic+

50 / 200

607190

10 ml pipetteshorty

2/10

10 ml

plastic / plastic+

50 / 200

www.gbo.com

Liquid Handling
CELLSTAR® Serological Pipettes

MaxiPette/

Ergonomic design for easy pipetting/

Variable speed control/

Cordless and rechargeable/

Up to eight hours of continuous operation/

Compatible with Greiner Bio-One serologicalpipettes

Description: MaxiPette, Content kit: pipette controller charger benchtop stand extra filter replaceable lithium battery

Item no.

Qty. inner / outer

847070

1/1/ Device with US or UK plug available on request

Pasteur / Serum Pipettes/

Ideally suited for the rapid transfer of liquids/

Sterile / non-sterile

Length: 153 mm
Item no.

Description

Graduation

Working volume

SterileQty. inner / outer

700370pasteur pipette-≤0,1 ml

700361

pasteur pipette-≤0,1 ml

612301

serum pipetteyes≤2,5 ml

612361

serum pipetteyes≤2,5 ml+

1 / 800

612362

serum pipetteyes≤2,5 ml+25 / 1.000

500 / 1.500+

25 / 1.000
500 / 1.500

www.gbo.com

/ 249

The Greiner Bio-One portfolio includes small laboratory equipment
to match the consumables such as PCR tubes, plates and tubes,
in order to provide our customers with comprehensive service,compatibility and highest quality: Mini-centrifuges, Vortex Mixers,
Block Heaters, Microplate Centrifuges and ergonomic pipettes areused for modern handling in everyday laboratory work. All from one
source - for highest quality requirements.

250 /www.gbo.com

Lab Equipment
﻿LAB EQUIPMENT/ Lab Equipment ���������������������������������� 252
Mini Centrifuge ���������������������������������������������253
Microplate Centrifuge �����������������������������������253
Mini Vortex Mixer �������������������������������������������254
Vortex Mixer ��������������������������������������������������254
Mini Block Heater ������������������������������������������255
Inserts for Mini Block Heater �������������������������255

www.gbo.com

/ 251

Lab Equipment
Lab Equipment/

For Sapphire MaxiPette pipette controller
please refer to chapter
Liquid Handling/

For further information, please refer to our
website: www.gbo.com/

Centrifuges/

Vortex Mixer/

Mini Block Heater

LAB EQUIPMENT
Greiner Bio-One, a leading provider of labware, completes its
product portfolio by compatible lab equipment.
Mini centrifuges are ideal for
quick spin-downs of microtubes and PCR tubes. Braking system is activated when the lid isopened, smoothly decelerating
to a complete stop in just one
second.
The microplate centrifuge allows centrifugation of two pla-

252 /www.gbo.com

tes, even without a lid or seal.
Our mini vortex mixer can vortex even the largest samples instantly with its 4 mm orbit andspeed of 2800 rpm. Despite its
powerful motor, it has a size
smaller than 10 x 10 cm, allowing
it to fit on even the most crowded bench.
The Mini Bock Heater is the perfect tool for labortory incubations, with its compact construction, broad temperature range
and multiple block options.

Lab Equipment
Lab Equipment

Mini Centrifuge/

Ideal for quick spin-downs of microtubes and
PCR tubes/

Rotor instantly reaches 6,000 rpm/

Storage compartment for PCR tube rotor/

Decelerates in just one second/

Compatible with all Greiner Bio-One PCR andreaction tubes

Feature: capacity 8 x 1.5 / 2.0 ml tubes, 4 x PCR strips (8 x 0.2 ml), 32 x 0.2 ml PCR tubes, Height: 11.4 cm, Length: 11.4 cm,
Width: 15 cm, Speed: 6,000 RPM, Operating temperature: 4 - 45 °C, Max. radius: 4.9 cm

Item no.

843070Plug

Qty. inner / outer

Europe- /1

Microplate Centrifuge/

Rapid spin-down of microplates with 2,550 rpm/

Unique rotor design holds two plates without
spillage/

50 % smaller than traditional centrifuges/

Compatible with most Greiner Bio-One microplates

Description: Microplate Centrifuge, Feature: capacity x 96 well microplates, 2 x 96 well PCR microplates,
Height: 19.7 cm, Length: 23 cm, Width: 26 cm, Speed: 2,550 RPM, Operating temperature: 4 - 45 °C, Max. radius: 8 cm

Item no.

846070Plug

Qty. inner / outer

Europe- /1/ Device with US or UK plug available on request

www.gbo.com

/ 253

Lab Equipment
Lab Equipment

Mini Vortex Mixer/

Powerful, quiet vortexing for tubes up to 50 ml/

Instant pressure-activated operation/

Compact, fits in the palm of your hand/Compatible with all Greiner Bio-One tubes

Description: Mini Vortex Mixer, Feature: head: Standard rubber lid for mixing single tubes, Height: 6.6 cm,
Length: 9.4 cm, Width: 9.9 cm, Mode: TOUCH Mix, Orbit: 4 mm, Operating temperature: 4 - 45 °C

Item no.

845070Plug

Qty. inner / outer

Europe- /1/ Device with US or UK plug available on request

Vortex Mixer/

Powerful motor for instant vortexing of up to 50 ml
tubes

/

Dynamic balance system to minimise noise andvibration/

Touch or continuous operation/

Variable speed control from 200 to 3,200 rpm/

Compatible with all Greiner Bio-One tubes

Description: Vortex Mixer, Feature: head: standard rubber cap single tube, base with four suction cups, Height: 17 cm,
Length: 13 cm, Width: 16 cm, Mode: ON (continuous), OFF and TOUCH mix, Orbit: 3 mm, Operating temperature: 4 - 45 °C

Item no.

844070Plug

Qty. inner / outer

Europe- /1/ Device with US or UK plug available on request

254 /www.gbo.com

Lab Equipment
Lab Equipment

Mini Block Heater/

Simple touchpad operation/

Easy-to-read digital display/

Compact, fits in the palm of your hand/

Exchangeable blocks for tubes from 0.2 to 50 ml

Description: Mini block heater (without insert), block options, high-grade aluminium,
Feature: ambient temperature from 5 - 100 °C, Height: 10 cm, Length: 11.2 cm, Width: 15 cm,
Temperature increments: +/- 0.5 °C, Temperature uniformity: 0.2 °C, Temperature incements: 0.1 °C,
Operating temperature: 4 - 45 °C
Item no.

848070Plug

Qty. inner / outer

Europe- /1/ Device with US or UK plug available on request

Inserts for Mini Block Heater/

Item no.

Exchangeable blocks for tubes from 0.2 to 50 ml

FeatureQty. inner / outer

848916

suitable for 15 pcs. 1.5 ml reaction tube, 0.5 ml PCR tube

- /1

848923

suitable for 15 pcs. 1.5 / 2 ml reaction tube, 0.5 ml PCR tube

- /1

848902

suitable for 40 pcs. 0.2 ml PCR tube, PCR 8-tube strip- /1848913

suitable for 15 pcs. 4 / 5 ml Cryo.s, 4.5 / 5 / 7 ml tube

- /1

848921suitable for 15 pcs. 1 / 2 ml Cryo.s, 4 ml tube

1/1

www.gbo.com

/ 255

Lab Equipment
Lab Equipment

Item no.

256 /FeatureQty. inner / outer

848915

suitable for 4 pcs. 15 ml conical tube, 12 / 14 / 20 ml tube

1/1

848950suitable for 2 pcs. 50 ml conical tube

1/1

www.gbo.com

Lab Equipment
Lab Equipment

www.gbo.com

/ 257

Certificate
Standard

ISO 9001:2015

Certificate Registr. No. 01 100 1412517
Certificate Holder:

Greiner Bio-One GmbH
Maybachstr. 272636 Frickenhausen
Germany

Scope:

Design and development, manufacturing and distributionof plastic products for diagnostic and pharma industry,
biotechnology and medicine

Proof has been furnished by means of an audit that therequirements of ISO 9001:2015 are met.

® TÜV, TUEV and TUV are registered trademarks. Utilisation and application requires prior approval.

Validity:

The certificate is valid from 2024-04-20 until 2027-04-19.
First certification 2014

2024-02-08
TÜV Rheinland Cert GmbHAm Grauen Stein · 51105 Köln

www.tuv.com

DIN EN ISO 9001 Certification

EN ISO 13485 Certification

DIN EN ISO 50001 CertificationQUALITY STANDARDS AT GREINER BIO ONE
Greiner Bio-One is certified
according to the international
standards
DIN
EN
ISO
9001 and EN ISO 13485 forMedical Devices. Since 2013,258 /www.gbo.com

Greiner
Bio-OneFrickenhausen (Germany) is also
certified according to DIN
EN ISO 50001 (systematicenergy management).

Technical Appendix

TECHNICAL APPENDIX/ General Information for the Lab..........260
Chemical Resistance of Various Materials�����260/ Laboratory Information forCentrifugation....................................280

Chemical Resistance of Various Materials����� 261

Centrifugation - Principle and Calculation

Chemical Resistance ofof the RCF

Cycloolefins (COC / COP)���������������������������������262(Relative Centrifugal Force)���������������������������280

Chemical Resistance of Polyethylene

Alignment Chart��������������������������������������������282

Terephthalate (PET) ��������������������������������������263

Maximal Centrifuge Capacity of Tubes, ���������282

Capillary Pore Membranes (ThinCert® Cell

Reaction Tubes and Microplates��������������������282

Culture Inserts)����������������������������������������������263

Reaction Tubes����������������������������������������������282

Chemical Resistance of Sealers��������������������264

Polystyrene Tubes�����������������������������������������283

Temperature Stability of Sealers�������������������264

Polyethylene Tubes����������������������������������������284

Physical Properties of Various Materials��������265

Multiwell Plates����������������������������������������������284

Manual Calculation����������������������������������������266

Microplates����������������������������������������������������284

Overview Metric Prefixes�������������������������������267

Microplates����������������������������������������������������285

�������������������������������������������������280

PCR Plates�����������������������������������������������������285/ Laboratory Information forMicroplates......................................... 268
Well Profiles of 96 Well Microplates���������������268/ Laboratory Information for Cryo.s
Sample Storage.................................. 286

Well Profiles of 96 Well ELISA Strip Plates�����270

Freezing Protocol������������������������������������������286
Thawing Protocol������������������������������������������287/ Laboratory Information for
Liquid Handling................................... 272
Compatibility for SAPPHIRE Pipette Tips
and Pipettes��������������������������������������������������272/ Laboratory Information for
Immunology........................................ 288
Volume-Dependent Wetting of
Immunological Products��������������������������������288/ Laboratory Information for PCR.......... 276
Overview PCR Microplates ����������������������������276/ Glossary.............................................290

Compatibility for PCR Microplates�����������������278
Compatibility for Mini Block Heater Inserts����280

www.gbo.com

/ 259

Technical Appendix

GENERAL INFORMATION FOR THE LABCHEMICAL RESISTANCE OF VARIOUS MATERIALS
Acetic acid 10 %Acetic acid 50 %Acetic acid 90 %AcetoneAcetonitrile
Ammonia 25 %Ammonium acetate
Amyl alcohol
Ascorbic acid
BenzeneBenzyl alcohol
Boric acid 10 %Carbon tetrachloride
Carbonic acid
Chloroform 100 %Citric acid 10 %Cyclohexanol
Detergents
Dichloroacetic acid
Diethyl ether
Dimethyl acetamide
Dimethylsulfox. (DMSO)
Emulsifier
Ethanol 50 %Ethanol 96 %EtherFormaldehyde 10 %Formaldehyde 40 %Formamide
Formic acid 50 %GlucoseGlycerine
HeptaneHexanolHydrochloric acid 20 %Hydrochloric acid conc.
Hydrogen peroxide 3 %Hydroquinone
Isoamyl alcohol
Isobutanol
Isopropanol
Isopropyl acetate
Isopropyl benzene
Isopropyl ether
Lactic acid 3 %Lactic acid 85 %Liquid paraffin

260 /www.gbo.com

PS 20 °C

PS 50 °C

PP 20 °C

PP 50 °C

1
2
4
4
4
2
1
1

1
2
4
4
4
2
1
1

4
4
1
4
1
4
1
3

4
4
1
4
1
4
1
3

1
1
1
1
3
1
1
1
1
4
4
1
4
1
3
1
1
1
1
4
1
1
1
1
1
4
1
1
1
1
1
1
3
1
1
1
1
1

1
1
2
3
4
1
1
1
1
4
4
1
4
1
4
1
3
1
1
4
1
1
1

4
4
1

4
4
2

1
1
4
3
4
1
3
1
1
4

1
1
4
4
4
1
3
1
1
4

1
3
1
4
1
2
2
4
4
4
2
2
1

1
3
1
4
1
2
2
4
4
4
2
2
1

1
1
2
3
4
1
1
1

HDPE 20 °C HDPE 50 °C LDPE 20 °C LDPE 50 °C

1
1
1
1
1
1
1
1
1
4
3
1
3
1
3
1
1

1
1

1
3
1
1

1
4
1
1
1

1
1
1

1
1
3
1
1
1
1
1
1
2
1
1
1
1

1
1
1

1
1
3
4
4
2
2
3

1
1
1
2
4
1
1
1

1
1
2
3
4
1
1
1

1
4
1
2
1
2
1
1
3

1
1
1
1
1
1
1
1
4
4
1
4
3

4
1
2
1
1
1
1
3

1
1
1
3
1
1
1
1
1
4
4
1
4
1
3
1
1

1
1
2
3
1
1
1
2
4
4
1
4
1
1
1

4
3
1

4
4
1

1
1
4
1
2
1
1
1
1
3
1
1
1
1
1

2

1
1
1
3

1
1
2
3
4
1
1
1

1
1
3
4
4
2
1
3

4
1
3
1
2
1
1
4

PC 20°C

PC 50°C

1
1
4
4
4
4
1
1
2
4
4
1
4
1
4
1
3

2
2
4
4
4
4
1
2
4
4
1
4
4
2

4
4

4
4

4

4

1
1
4
1
1
3
3
1
3
1
2
2
4
3
3
3
1
1
4
4
4
1
1
1

1
3
4
2
2
3
3
1
3
2
2
3
4
3
3
0
2
2
4
4
4
2
2

Technical appendix is subject to error and technical modifications.

Technical Appendix

CHEMICAL RESISTANCE OF VARIOUS MATERIALSPS 20 °C

PS 50 °C

PP 20 °C

PP 50 °C

HDPE 20 °C HDPE 50 °C LDPE 20 °C LDPE 50 °C

Methanol

3

4

1

1

1

1

1

Methyl acetate

4

4

2

3

3

3

3

3

1

2

4

4

4

Methyl phenyl ether 100 %

4

4

3

Methyl propyl ketone

4

4

2

4

4

3

1

3

Nitrobenzene

4

4

4

4

3

Oxalic acid

1

1

1

1

Ozone

3

3

1

2

Palmitic acid

1

1

3

4

3

Phenol 10 %

4

4

1

1

1

1

1

Phenol 100 %

4

4

1

1

2

3

3

Phosphoric acid 1 – 5 %

2

2

1

1

1

1

Phosphoric acid 85 %

1

1

1

2

1

1

Phthalic acid

1

1

1

1

1

Potassium carbonate

1

1

1

1

1

Potassium chromate

1

1

1

1

Potass. permanganate

2

3

1

1

Propanol

3

3

1

Sodium acetate

2

2

1

Sodium chloride

1

1

Sodium hydroxide 30 %1

Sodium hydroxide 45 %1

Sodium hydroxide 60 %Sodium hypochloride

Methylamine 32 %Methylene chloride

PC 20°C

PC 50°C

1

4

4

4

4

4

4

4

4

4

4

4

4

4

3

1

1

2

3

1
4

4

3

3

4

4

4

4

4

1

1

1

1

3

4

1

1

1

2

1

2

2

2

1

4

4

3

4

4

1

1

1

1

1

1

1

2

1

1

1

3

3

1

1

1

3

3

1

1

1

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

4

4

1

1

1

1

1

1

1

4

4

1

1

1

1

4

4

1

1

2

3

2

3

2

3

2

3

Sodium permanganate

2

3

1

1

1

1

1

1

Sodium thiosulfate

1

1

1

1

1

1

1

1

2

2

Stearic acid

1

2

1

1

1

1

1

1

1

2

Sulphuric acid 1 – 6 %

1

2

1

1

1

1

1

1

1

1

Sulphuric acid 60 %

2

4

1

3

1

3

1

3

3

3

Sulphuric acid conc.

4

4

4

4

4

4

4

4

4

4

Tannin acid

1

1

1

1

3

3

3

4

3

4

4

4

4

3

4

4

4

4

4

Naphthalene1

Terpentine oil
Tetrahydrofuran

4

4

3

2

Toluene

4

4

3

4

3

4

3

4

4

4

Trichloroacetic acid

4

4

3

4

3

3

3

4

4

4

Urea

1

2

1

1

1

1

1

1

1

1

Uric acid

1

1

1

1

Urine

3

3

1

1

1

1

1

1

1

Xylene

4

4

4

4

2

3

2

4

4

1 = resistant2 = limited resistant 3 = moderate resistant 4 = no resistance

4

These tables are a general guide only. As many factors can affect the chemical resistance of a given product, its suitability for a specific application should be tested.

Technical appendix is subject to error and technical modifications.

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Technical Appendix

CHEMICAL RESISTANCE OF CYCLOOLEFINS (COC / COP)
Cycloolefin

Cycloolefin

Cycloolefin

Acetic acid 99 %1

Dibutyl ether

4Isopropanol

1

Acetone1

Dichloroethane

4

Methanol1

Acrylonitrile

1

Dichloromethane

4

Methylene chloride

4

Ammonia 33 %1

Diethyl ether

4

Nitric acid (HNO3)

1

Benzaldehyde

3

Dimethyl sulfoxide1Octane4

Benzene

4

DMSO

1

Pentane4

Benzine4

Ethanol 50 %1

Sodium hydrox. (NaOH) 50 %1

Butanon1

Ethanol 96 %1

Sulphuric acid (H2SO4) 40 %1

Carbon tetrachloride

4

Fatty acid4

Sulphuric acid (H2SO4) 95 %1

Chloroform

4

Heptane (n-Heptane)

4

Toluene4

Cyclohexane

4

Hexane4

Xylene4

Cyclohexanone

4

Hydrochloric acid (HCl) 36 %1

Detergents1

Hydrogen peroxide water 30 %1

1 = resistant2 = limited resistant 3 = moderate resistant 4 = no resistanceThis table is a general guide only. As many factors can affect the chemical resistance of a given product, its suitability for a specific application should be tested.

262 /www.gbo.com

Technical appendix is subject to error and technical modifications.

Technical Appendix

CHEMICAL RESISTANCE OF POLYETHYLENE TEREPHTHALATE (PET)CAPILLARY PORE MEMBRANES (THINCERT® CELL CULTURE INSERTS)
PET

PET

PET

Acetaldehyde

1Ethanol

1

Monochlorbenzene

1

Acetic acid (10 %)1Ethyl acetate

1

Nitric acid (30 %)1

Acetic acid (100 %)3

Ethyl ether

1

Nitrobenzene

1

Acetone1

Ethylendichloride

1

Nitropropane

1

Ammonium hydroxide (5 %)1Ethylene glycol

1n-Propanol

1Amyl acetate

1

Fluoric acid (35 %)1

Pentane1

Amyl alcohol1

Formaldehyde

1Perchlorethylene

1Aniline1

Formic acid (50 %)1

Petroleum ether

1

Benzene

3

Freon

1

Phosphoric acid (85 %)3

Benzyl alcohol

1

Glutaraldehyde

1

Potassium hydroxide

4

Benzyl benzoate

1

Glycerol1

Propyl acetate

1

Boric acid (5 %)1

H2O2 (30 %)1

Pyridine

1

Butanol

1

Halogenated phenoles

4

Silicon oil

1Butyl acetate

1

Hexane1

Sodium hydroxide

4Butyl cellusolve

1

Hydrochloric acid (20 %)1

Sulphuric acid (25 %)1

Carbon tetrachloride

1

i-Propanol1

Terpentine oil

1

Chloroform

1Isopropyl myristate1

Tetrahydrofurane

1Concentrated strong acids

4

Methanol1

Tetraline

1

Cyclohexane

1

Methyl acetate

1

Toluene3

Cyclohexanone

3

Methyl cellusolve

1

Trichlorbenzene

1

Dekaline

1

Methylenchloride

3

Trichlorethylene

1

Dimethylacetamide

1

Methylethylketone

1

Triethanolamin

1

Dimethylformamide

1

Methylglycol acetate1

Trikresyl phosphate

1

Dimethylsulfoxide

1Methylisobutylketone

1

Xylene3

Dioxane1

Mineral oils

1

For the solvents effecting slight changes the user should test the compatibility under the specific application conditions. All tests have been performed at RT. Please beaware that ThinCert® cell culture inserts are made of PET membranes sealed on polystyrene housings. Therefore, solvents shown compatible with PET membranes in theabove table might be incompatible with the polystyrene housing. Please check solvent compatibility with polystyrene.
Resistance scale from 1 to 4
1 = resistant				i.e. the plastics may be treated with the chemical compound at mentioned temperature over several years
any significant alterations in its physical, optical and chemical properties

2 = limited resistant		

i.e. the plastics may be treated with the chemical compound at mentioned temperature over several weeks without any
significant alterations in its physical, optical and chemical properties

3 = moderate resistant		

i.e. the plastics may be treated with the chemical compound at mentioned temperature for short time only (several minutes to one hour)
without any alterations in physical, optical and chemical properties (mixing and measuring is possible)

4 = no resistancei.e. treating the plastics with the substance named may cause alterations in physical, optical and chemical properties within seconds

Technical appendix is subject to error and technical modifications.

without 		

www.gbo.com

/ 263

Technical Appendix

CHEMICAL RESISTANCE OF SEALERS
EASYseal(Art. No. 676001)

VIEWseal(Art. No. 676070)

AMPLIseal(Art. No. 676040)

SILVERseal(Art. No. 676090)

Acetone

4

4

4

3

Acetonitrile

3

3

4

1

Acetic acid 1 %

3

1

4

3

Glacial acetic acid

1

3

4

3

Chloroform

4

4

4

4

DMSO

3

3

3

1

Ethanol

3

1

1

1

Hydrochloric acid 32 %

3

1

3

4

Isopropanol

3

1

1

1

Methanol

3

1

4

1

Phenol33

43Sulphuric acid 0.5 M

1

1

1

1

1 = Stable		
3 = Moderately stable
4 = Unstable		

no visible change in the sealer after one week‘s incubation
after one week, optical and physical changes in the sealer (clouding tears on removal)
adhesive and foil are dissolved, wells not leak-tightThis table can only be used as an orientation aid for the suitability of the respective sealers, since their behaviour against chemicals depends on the respective application.
Tests under practical conditions are absolutely essential in many cases.

TEMPERATURE STABILITY OF SEALERS
Temperature Stability

EASYseal-40 °C to + 120 °C

VIEWseal-70 °C to + 100 °C

AMPLIseal-80 °C to + 110 °C

SILVERseal-70 °C to + 100 °C

BREATHseal

n.a. / Evaporation rate 4200 g H2O/m² in 24 hThis table can basically be used as an orientation aid for the temperature stability of the respective sealers, since the behaviour of the product depends on the respective
application. Tests under practical conditions are absolutely essential in many cases.

264 /

www.gbo.com

Technical appendix is subject to error and technical modifications.

Technical Appendix

PHYSICAL PROPERTIES OF VARIOUS MATERIALS
Material

Autoclavability

Sterilisation by
gamma
irradiation

chemicals(formalin,ethanol)

Polystyrene

•

•

•

Polypropylene

•

•

•

dry heat

ThermalStability [°C]

gas 1

•

Gas
Permeability 2

Transparency

WVTR 3

O2

N2

CO2

-20 to +60

clear

4.7

853

17.8

108 – 155

-196 to +121translucent

3.7

744

12.4

3.9
4.6 – 6.2

HDPE

•

•

•

-50 to +100translucent

2.9

651

9

LDPE

•

•

•

-50 to +80

translucent

7.8

2.8

41.9 15.5 –23.3

UV-Star®

••-20 to +40

clear

PETG

•

•

•

-40 to +60

clear

388

155

1.2

62

PET

•

some

•

-40 to +60

clear

46.5

10.9

236

15 – 20

Cycloolefin••-80 to +100

clear

Exemptions are mentioned in the respective product data sheets.
123Ethylene oxide, formaldehyde[cc x mm/m 2 x 24 h x Bar]at 37 °C, 90 % humidity [g x mm/m 2 x 24 h x Bar]

Material

Refractive Index

Polystyrene

1.59

UV-Star®

1.53

Cycloolefin

1.53

Glass

1.53

These tables are a general guide only. As many factors can affect the resistance of a given product, its suitability for a specific application should be tested.

Technical appendix is subject to error and technical modifications.

www.gbo.com

/ 265

Technical Appendix

MANUAL CALCULATION
COEFFICIENT OF VARIATION (CV)The coefficient of variationcomparesthevariabilityof several random samples
with different means, taking into account the different
dimensions of means:

CV % =S
IXI· 100 %/

where S is the standard
deviation and I X I is theabsolute value of thearithmetic mean.

/

This formula can beused for calculating thefilling level in relation
to the filling volume in a
96 well microplate withcylindrical wells.

/

This formula can beused for calculating thefilling level in relation to
the filling volume in 384
and 1536 well
microplates withrectangular wells.

VOLUME OF DIVERSE BODIESVOLUME OF A CYLINDER:V=h=

π · d2 · h
4

4·V
π

VOLUME OF A CUBOID:

V=a·b·hh=266 /www.gbo.com

Va·b

Technical appendix is subject to error and technical modifications.

Technical Appendix

OVERVIEW METRIC PREFIXES

G =
M =
k =
c =
m =
μ =
n =
p =
f =
a =
z =

giga
mega
kilo
centi
milli
micro
nano
pico
femto
atto
zepto

=
=
=
=
=
=
=
=
=
=
=

10 9
10 6
10 3
10 –2
10 –3
10 –6
10 –9
10 –12
10 –1510 –18
10 –21

Technical appendix is subject to error and technical modifications.

www.gbo.com

/ 267

Technical Appendix

LABORATORY INFORMATION FOR MICROPLATESWELL PROFILES OF 96 WELL MICROPLATES
96 Well Polystyrene Microplates

Clear

U-Bottom

V-BottomWhite

F-BottomHalf Area

F-BottomµClear®

Black

Half Area

F-BottomµClear®

Half Area

WELL PROFILE

V-BOTTOM

The well profile is a critical aspect in a 96 well
microplate. Different well shapes are available
for each application (Fig. 1 – Fig. 4):

The “V” stands for the conically tapered wellbottom (Fig. 2). These microplates are ideallysuited for applications in which the entire
sample volume must be pipetted off.

U-BOTTOM/ For precise pipetting

The “U” describes the round bottom shape(Fig. 1). U-bottom microplates are ideallysuited for agglutination tests.

/ Ideally suited for the storage of samples/ Suitable for +/- analyses/ No sharp corners to facilitate easy
and residue-free pipetting/ Suitable for +/- analyses

Figure 2:
Well profile: 96 well V-bottom, polystyrene
Total volume: 234 µl
Working volume: 40 – 200 µl

Figure 1:
Well profile: 96 well U-bottom, polystyrene
Total volume: 323 µl
Working volume: 40 – 280 µl

268 /www.gbo.com

Technical appendix is subject to error and technical modifications.

Technical Appendix

F-BOTTOM / STANDARD (ST)HALF AREA

The “F” refers to the flat well bottom (Fig. 3).
This well type is ideal for precise opticalmeasurements. The measuring light source is
not deflected by the well profile.

Half Area microplates (Fig. 5) offer an interesting alternative to conventional 96 well
microplates. They can be pipetted manually
without any problem but at the same time allow
a reduction of the sample volume by up to 50 %.

/ Excellent optical properties/ For precise optical measurements/ For microscopic applications(bottom reading)/ Reduction of sample volume of up to 50 %/ Standardised pathlength(1 cm=170 µl, 0.5 cm=80 µl)////
Figure 3:
Well profile: 96 well F-bottom / ST, polystyrene
Total volume: 382 µl
Working volume: 25 – 340 µl
Growth area: 32 mm2Figure 5:
Well profile: 96 well Half Area
Total volume: 199 µl
Working volume: 15-175 µl
Growth area: 15 mm²

F-BOTTOM / CHIMNEY WELL
The standard flat bottom microplate (Fig. 3)
has the same well profile as the chimney well
microplate (Fig. 4). The difference from thestandard plate is the chimney-like arrangementof the wells. Each well stands on its own.
Therefore the risk of sample carryover andcross contamination is minimised.

Figure 4:
Well profile: 96 well F-bottom / chimney well,
polystyrene
Total volume: 392 µl
Working volume: 25 – 340 µl
Growth area: 34 mm²

Technical appendix is subject to error and technical modifications.

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/ 269

Technical Appendix

WELL PROFILES OF 96 WELL ELISA STRIP PLATES
96 Well ELISA Strip Plates

C-Bottom SB Strip Plates

C8

F-Bottom Strip Plates

F8

U-Bottom Strip Plates

F16

U8

U16

WELL PROFILE
Microplates in strip format offer the advantage
of greater flexibility in diagnostics. Individual
strips can be removed from the support frameso that the number of tests to be performed
can be adjusted to the number of samples, andis not predetermined by the microplate format
used. In addition, the individual strips of a
microplate can be subjected to a wide variety
of different test conditions.

Figure 1:
Well profile: C-bottom
Total volume: 346 µl
Working volume: 20-300 µl

C-BOTTOM SB (SINGLE-BREAK)STRIP PLATESThe “C” describes a flat-bottom profile withrounded corners (Fig. 1). The rounded corners
mean that the individual wells can be pipetted
without leaving a residue, and the flat bottom
still enables precise optical measurements.
C-bottom SB strips are supplied as twelve
8 well strips, in a support frame with 96 spaces(12 x 8 matrix). The individual wells can be
broken off separately (“single-break” option)and the number of tests performed can thus be
precisely adjusted to the number of samples.
C-bottom SB strip plates are available in clear
polystyrene and additionally with a red, green,
blue or yellow colour coding on the well rim.

270 /

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Technical appendix is subject to error and technical modifications.

Technical Appendix

U-BOTTOM STRIP PLATESF-BOTTOM STRIP PLATES

96 well strip plates with a round bottom(U-bottom, Fig. 2) are available as both 8 welland 16 well strips. The “U” describes the roundbottom form. U-bottom strip plates are ideallysuited for agglutination tests.

96 well strip plates with a flat bottom(F-bottom, Fig. 3) are available as 8 well strips
and as 16 well strips. The “F” stands for theflat bottom of the wells. This well type is ideal
for the most precise optical measurements
as the measuring light beam is not deflectedby the well profile. F-bottom strip plates areavailable in clear polystyrene (MICROLON) andadditionally in black (FLUOTRAC) and white(LUMITRAC).

/ No corners, therefore simple andclean pipetting/ Suitable for +/- analyses

Figure 2:
Well profile: U-bottom
Total volume: 312 µl
Working volume: 50-280 µl

Technical appendix is subject to error and technical modifications.

Figure 3:
Well profile: F-bottom
Total volume: 388 µl
Working volume: 20-350 µl

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Technical Appendix

LABORATORY INFORMATION FOR LIQUID HANDLINGCOMPATIBILITY FOR SAPPHIRE PIPETTE TIPS AND PIPETTESPIPETTE TIPS (STANDARD / LOW RETENTION)
Volume [µl]1010 extended

20

100

200

300

1000

1250

Volume range [µl]0.2-100.5-10

2-20

2-100

5-200

10-30050-1000

50-1250

Tip version

Tip/
Filter tip

Tip/
Filter tip

Filter tip

Filter tip

Tip/
Filter tip

Tip/
Filter tip

Tip

Tip/
Filter tip

Article no.

7713XX

7723XX

7733XX

7743XX

7753XX

7763XX

7773XX

7783XXT l F-













PIPETTE MODELS
GREINER BIO-ONE
Single-channel pipettes
Sapphire

0.2-2 µl1-10 µl2-20 µl10-100 µl

T l F-

20-200 µl

T l F-

100-1000 µl
500-5000 µl
1000-10000 µl
Multi-channel pipettes
Sapphire

8/12 CH 0.5-10 µl

8/12 CH 2-20 µl

8/12 CH 20-200 µl









8/12 CH 20-300 µl









T l F-

BRAND
Single-channel pipettes
Transferpette® S

0.5-10 µl

2-20 µlT l F-

10-100 µl

20-200 µl

T l F

100-1000 µl
EPPENDORF
Single-channel pipettes
Reference®

0.5-10 µl

2-20 µlT l F-

10-100 µl

T l F-

50-200 µl

100-1000 µl
Research®

0.5-10 µl

2-20 µlT l F-

10-100 µl

T l F-

20-200 µl









100-1000 µl
Research® plus

0.5-10 µl
10-100 µl
20-200 µl
100-1000 µl

272 /www.gbo.com








T l F-

Technical appendix is subject to error and technical modifications.

Technical Appendix

PIPETTE TIPS (STANDARD / LOW RETENTION)
Volume [µl]1010 extended

20

100

200

300

1000

1250

Volume range [µl]0.2-100.5-10

2-20

2-100

5-200

10-30050-1000

50-1250

Tip version

Tip/
Filter tip

Tip/
Filter tip

Filter tip

Filter tip

Tip/
Filter tip

Tip/
Filter tip

Tip

Tip/
Filter tip

Article no.

7713XX

7723XX

7733XX

7743XX

7753XX

7763XX

7773XX

7783XXT l F-1000 µl

500 µl

















PIPETTE MODELS
Research® pro

0.5-10 µl
5-100 µl20-300 µlFixed-volume pipettes

Reference®

50 µl



T l F-100 µlT l F-500 µlResearch®

Multi-channel pipettes
Eppendorf

8CH 10-200 µl

Research®

12CH 0.5-10 µlResearch® plus

8CH 0.5-10 µl

8CH 10-100 µl
8CH 20-300 µl































Electronic pipettes
Xplorer®

50-1000 µl
8CH 15-300 µl

GILSON
Single-channel pipettes
Pipetman®

P2 (0.5-2 µl)

P10 (1-10 µl)

P20 (2-20 µl)

P100 (20-100 µl)







P200 (50-200 µl)







T l F-

P1000 (200-1000 µl)
Pipetman® L

P2L (0.2-2 µl)



P10L (1-10 µl)



P20L (2-20 µl)

T l F-

P200L (20-200 µl)P1000L (100-1000 µl)Fixed-volume pipettes
Pipetman®

F5 (5 µl)T l F-

F10 (10 µl)

T l F-

F20 (20 µl)



F25 (25 µl)

T l FT l F-

T = Pipette tip

 compatible

F= Filter tip

 Pipetting volume limited to the maximum volume of the pipette tips

Technical appendix is subject to error and technical modifications.

- not compatible

www.gbo.com

/ 273

Technical Appendix

PIPETTE TIPS (STANDARD / LOW RETENTION)
Volume [µl]1010 extended

20

100

200

300

1000

1250

Volume range [µl]0.2-100.5-10

2-20

2-100

5-200

10-30050-1000

50-1250

Tip version

Tip/
Filter tip

Tip/
Filter tip

Filter tip

Filter tip

Tip/
Filter tip

Tip/
Filter tip

Tip

Tip/
Filter tip

Article no.

7713XX

7723XX

7733XX

7743XX

7753XX

7763XX

7773XX

7783XXPIPETTE MODELSGILSON (continued)
Fixed-volume pipettes
Pipetman®

F100 (100 µl)

T l F-

F200 (200 µl)F250 (250 µl)

F300 (300 µl)

F500 (500 µl)

F1000 (1000 µl)







Multi-channel pipettes
Pipetman® L

P8X 10L (0.5-10 µl)



P8X 200L (20-200 µl)









P8X 300L (20-300 µl)









IKA
Single-channel pipettes
IKA Pette vario

100-1000 µl



METTLER TOLEDO
Single-channel pipettes
Rainin Pipette-Lite

TM

SL200 (20-200 µl)









Rainin Pipette-Lite TM SL1000 (100-1000 µl)













NICHIRYO
Single-channel pipettes
Nichipet

0.5-10 µl

2-20 µlT l F-

10-100 µl









20-200 µl









100-1000 µl
Multi-channel pipettes
Nichipet

8K 30-300 µl







SARTORIUS
Single-channel pipettes
Biohit Proline®

0.5-10 µl

Biohit Proline® plus

0.5-10 µl

2-20 µl10-100 µl









20-200 µl









T l F-

100-1000 µl
Multi-channel pipettes
Biohit Proline®

8CH 0.5-10 µl

8CH 25-250 µl
8CH 50-300 µl











SOCOREX
Single-channel pipettes
Acura® 825

274 /0.5-10 µl

www.gbo.com

Technical appendix is subject to error and technical modifications.

Technical Appendix

PIPETTE TIPS (STANDARD / LOW RETENTION)
Volume [µl]1010 extended

20

100

200

300

1000

1250

Volume range [µl]0.2-100.5-10

2-20

2-100

5-200

10-30050-1000

50-1250

Tip version

Tip/
Filter tip

Tip/
Filter tip

Filter tip

Filter tip

Tip/
Filter tip

Tip/
Filter tip

Tip

Tip/
Filter tip

Article no.

7713XX

7723XX

7733XX

7743XX

7753XX

7763XX

7773XX

7783XX













PIPETTE MODELS
2-20 µlT l F-

5-50 µlT l F-

10-100 µl

T l F-

20-200 µl









100-1000 µl
Calibra® 822

1-10 µl2-20 µl10-100 µl

20-200 µl

T l FT l F100-1000 µl
Multi-channel pipettes
Acura® 855

8CH 5-50µl

Calibra® 852

8CH 1-10 µl

T l F-

8CH 20-200 µlT l F-

12CH 10-100 µl







T l F-STARLAB
Single-channel pipettes
ErgoOne®

0.1-2.5 µl
0.5-10 µl

10-100 µl

T l F-

100-1000 µl
Multi-channel pipettes
ErgoOne®

8 CH 30-300 µl







T l F-

THERMO FISHER SCIENTIFIC
Single-channel pipettes
FinnpipetteTM F1

1-10 µl2-20 µl20-200 µl30-300 µl

FinnpipetteTM 4500

100-1000 µl

200-1000 µl













Multi-channel pipettes
FinnpipetteTM F1 MCP8 8 CH 1-10 µl

8 CH 10-100 µl







8CH 30-300 µl







Finnpipette 4510

8CH 50-300 µl







FinnpipetteTM 4500

8CH 200-1000 µl

TM



VWR
Single-channel pipettes
Erg. High Perform.

0.5-10 µl

Erg. High Perform.

2-20 µlErg. High Perform.

10-100 µlErg. High Perform.

20-200 µlErg. High Perform.

100-1000 µlTechnical appendix is subject to error and technical modifications.

T l F-www.gbo.com

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Technical Appendix

LABORATORY INFORMATION FOR PCROVERVIEW PCR MICROPLATESThe use of the 96 well format allows thescale up of basic PCR work, while the 384
well format is ideal for high-throughput
screening projects. All microplates are madeof thin-walled polypropylene. This optimises
the heat transfer from the thermoblock to

the reaction solution. Our heat-resistantsealers AMPLIseal, VIEWseal and SILVERseal
are ideal for sealing the microplates
during PCR, and the 96 well microplate
may also be sealed with 8-cap strips .

96 WELL POLYPROPYLENE
MICROPLATES FOR PCR1c) Non-skirted microplate, low profile(Art. No. 652210)

1/ NON-SKIRTED MICROPLATES
Non-skirted microplates may be used in allcommonly available thermocyclers with a
96 well block.

1a) Non-skirted microplate with raised wellrims (Art. No. 652201)

Figure 1a: View of a non-skirted microplatewith raised well rims1b) Non-skirted microplate withflat surface (Art. No. 652250)

Figure 1c: View of a non-skirted low profile
microplate
2/ HALF-SKIRTED MICROPLATES2a) Half-skirted microplate with one notch
suitable for real-time PCR systems such as
LightCycler® 480 (Art. No. 669285)/ Maximal pigmented white polypropylene
and therefore most suitable for sensitive
real-time PCR reactions/ B lack alphanumeric coding enables a quick
identification of samples/ Notches in the rim facilitate automation
due to better gripping in robotic systems

Black alphanumeric coding enables a quick
identification of samples

Figure 1b: View of a non-skirted microplatewith flat surface

Figure 2a: View of a half-skirted microplatewith one notch suitable for real-time PCRsystems such as LightCycler® 480 from Roche

The registered trademarks of the mentioned manufacturers belong to the above mentioned companies.

276 /www.gbo.com

Technical appendix is subject to error and technical modifications.

Technical Appendix2b) Half-skirted microplate with one notch
suitable for ABI (Art. No. 652290)

Figure 2b: View of a half-skirted microplatewith one notch suitable for ABI

3/ FULL-SKIRTED MICROPLATE WITH
ONE NOTCH (ART. NO. 652270)

Figure 3: View of full-skirted microplate with
one notch

2c) Half-skirted microplate, recessed rim,
ABI design with one notch (Art. No. 652260)

Figure 2c: View of a half-skirted microplate,recessed rim, ABI design with one notch

384 WELL POLYPROPYLENE
MICROPLATES FOR PCR

2/ FULL-SKIRTED 384 WELL
MICROPLATE WITH TWO NOTCHES(ART. NO. 785201)

The 384 well PCR microplates from Greiner
Bio-One are manufactured in an advanced injection moulding process following stringent
quality criteria. Minimal distortion and sagging curvature, homogeneous heat transfer
and sealing of the individual wells are essential quality criteria here. The footprint of all
384 well PCR microplates is compatible with
automated systems.

Figure 5: Full-skirted 384 well microplate with
two notches and alphanumeric coding
3/ FULL-SKIRTED 384 WELL MICROPLATE WITH TWO NOTCHES FOR REALTIME PCR SYSTEMS (ART. NO. 785285)

1/ FULL-SKIRTED 384 WELL
MICROPLATE WITH ONE NOTCHSUITABLE FOR ABI(ART. NO. 785290)/ White pigmentation boosts real-time
PCR signal

Figure 4: Full-skirted 384 well microplate with
one notch and alphanumeric coding suitablefor ABI

Figure 6: Full-skirted 384 well microplate with
two notches for real-time PCR systems suchas LightCycler® 480 from Roche/ Black alphanumeric coding enables a 		quick identification of samples

The registered trademarks of the mentioned manufacturers belong to the above mentioned companies.

Technical appendix is subject to error and technical modifications.

www.gbo.com

/ 277

Technical Appendix

96 well
2700

Cyclers

•

•

•

•

•
•

•
•

•

•
•

•
•
•
•
•
•

•

•

•
•

6100
9600
9700

•

Veriti 0.2 ml

Applied Biosystems

5700
PRISM 7000
Real Time
Thermal
Cyclers

7300
7500
7700
7900HT••••

ViiA 7••

Quant Studio 12K Flex
PRISM 310
PRISM 3100
Sequencers

3130 (XL)3700
PRISM 3730 (XL)

•
•
•
•
•

•
•
•
•
•

•
•
•
•
•
•
•

•
•
•
•
•
•
•
•
•

•

•
•

3500 (XL)
Gene Cycler
PTC-100
PTC-200
PTC-225 Tetrad
Dyad/Dyad Disciple
ThermaliCycler

Cyclers

MyCycler
Mini Gradient

Bio-Rad / MJ Research785290skirted,suitablefor ABI

384 well

2720
Thermal785285skirted,
RT-PCR

785201skirted669285semi-skirted,
RT-PCR

652290semi-skirted,
suitablefor ABI652270skirted652260semi-skirted,
ABI-Design

652210unskirted,
low-profile

Model

652250unskirted,
universal

Optimal Fit

652201
unskirted,chimney top

COMPATIBILITY FOR PCR MICROPLATESPersonal

•

•
•
•
•

•
•
•
•

•
•
•
•

•
•
•

T100

•
•
•

DNA Engine family
C1000/S1000
Opticon/Opticon2
Chromo-4
Real Time
Thermal
Cyclers

iCycler
MyiQ
iQ5

•
•
•

•

•
•

•
•

•

•
•
•
•
•

•

•
•

•
•

•
•
•

CFX Connect

•
•

•
•

•

•

•

•

•

•

CFX96
CFX384
Sequencers

BaseStation
Mastercycler

Eppendorf

Mastercycler ep Gradient
Thermal
Cyclers

•
•

•
•

•

•
•
•

•

•
•
•
•
•

•
•

Mastercycler M384
Mastercycler Nexus
Mastercycler Nexus Gradient
Mastercycler Nexus Eco
Mastercycler Pro

Real Time T.C.

•

Mastercycler ep Realplex

•
•
•

•
•
•
•
•

The registered trademarks of the mentioned manufacturers belong to the above mentioned companies. This compatibility chart is a general guide only and subject to error
and modifications. We cannot accept any liability or responsibility for the above information.

278 /www.gbo.com

Technical appendix is subject to error and technical modifications.

96 wellAmersham
BeckmannSequencers

MegaBACE 1000

BiometraThermal
Cyclers

T1 Thermal Cycler
Tgradient
Trobot

•
•
•
•
•

•
•
•
•
•
•
•
•

•
•
•
•
•
•
•
•

•
•
•
•
•
•
•
•
•
•
•
•
•
•

•
•
•
•
•
•
•
•
•
•
•
•
•
•

•
•
•
•
•

•
•

•
•
•

T3000

Corbett

T Professional
Real Time T.C.

Toptical

Thermal
Cyclers

PalmCycler 384

EricomThermal
Cyclers

Deltacycler I
Deltacycler II
Single Block

Esco

Twin Block
Thermal
Cyclers

Swift
Gene
Genius

G-Storm
MWG

GS1
Thermal
Cyclers

Thermal
Cyclers

GS2
GS4
GSX

TaKaRaStrategene

GSXs
Primus 96
Robocycler 96
Robocycler Gradient

Real Time T.C.

Mx4000 and Mx3005P

Thermal
Cyclers

TP3000Cyclogene

TechneGeniusGenius Quad
Genius (TC412)
Flexigene

•
•
•
•
•
•
•
•

•
•
•
•
•
•
•
•
•

•
•
•
•
•
•

•
•
•
•
•
•

Touchgene X
Touchgene Gradient (TC512)
Quantica
PCR Sprint
MBS Satelite (Multiblock)Thermal
Cyclers

Px2 and PxE
PCR Express and Omni-E
Touchdown
Omnigene

Sequencers
Real Time T.C.

•
•
•
•
•
•

•

•
•
•
•
•
•

•
•
•
•
•
•
•

•

•

•

•

•
•

•
•

•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•

•
•
•
•
•
•
•
•
•

TP240
Touchgene

Real Time T.C.

•
•
•
•
•
•
•

•
•
•
•
•

•

•
•
•
•
•
•
•
•
•
•
•

•

•
•

•
•

•
•

•

Primus 384

Thermal
Cyclers

Thermal
Cyclers•

PalmCycler 96
Power Block I

WAVE System
LightCycler 96
Light Cycler 480

785290skirted,suitablefor ABI

•

CEQ
Uno

Thermo Hybaid•

MegaBACE 4000

Uno II

Ro- Transche genomic

384 well••

MegaBACE 500
Sequencers

785285skirted,
RT-PCR

785201skirted669285semi-skirted,
RT-PCR

652290semi-skirted,
suitablefor ABI652270skirted652260semi-skirted,
ABI-Design

652210unskirted,
low-profile

Model

652250unskirted,
universal

Optimal Fit

652201
unskirted,chimney top

Technical Appendix

•
•
•
•
•
•

•
•

•
•
•
•
•
•
•
•
•
•
•

•
•
•
•
•
•
•
•
•

•
•
•

•
•
•
•
•
•

•
•
•
•
•

•
•
•

•
•

•

•

The registered trademarks of the mentioned manufacturers belong to the above mentioned companies. This compatibility chart is a general guide only and subject to error
and modifications. We cannot accept any liability or responsibility for the above information.

Technical appendix is subject to error and technical modifications.

www.gbo.com

/ 279

Technical Appendix

COMPATIBILITY FOR MINI BLOCK HEATER INSERTS
Art. no.

848916848923848902848913848921

848915848950No. of Tubes per Insert

15x

15x

40x

15x

15x

4x

2x

1 ml Cryo.s•

2 ml Cryo.s•

4 ml Cryo.s•

5 ml Cryo.s•

1.5 ml Reaction Tube•

2 ml Reaction Tube
0.5 ml PCR Tube

•
•

•

•

0.2 ml PCR Tube•

1 x 8 PCR Tube Strip•

15 ml Conical Tube•

50 ml Conical Tube•

4 ml, 12 x 55 mm Tube•

5 ml, 12 x 75 mm Tube•

4.5 ml, 12 x 75 mm Tube•

7 ml, 13 x 100 mm Tube•

12 ml, 16 x 100 mm Tube•

12 ml, 17 x 100 mm Tube•

20 ml, 16 x 152 mm Tube•

14 ml, 17 x 95 mm Tube•

14 ml, 18 x 95 mm Tube•

This compatibility chart is a general guide only and subject to error and modifications. We cannot accept any liability or responsibility for the above information.

LABORATORY INFORMATION FOR CENTRIFUGATIONCENTRIFUGATION - PRINCIPLE AND CALCULATION OF THE RCF(RELATIVE CENTRIFUGAL FORCE)
Sedimentation of particles ina gravitational fieldIf a mixture of sand and wateris shaken thoroughly and thenleft to stand, the sedimentation of the solid particles
takes place according totheir size. As a result ofgravitational
acceleration(g = 9.81 m / s2), all of theparticles are located in a
gravitational field under the
influence of which the coarse
280 /www.gbo.com

grains of sand collect at thebottom first and the smaller
grains of sand are depositedlater. After around 10 – 20
minutes, the following layering
is produced (from bottom
to top): coarse grains of sand– fine grains of sand – water.
However, other particles(proteins, nucleic acids,
viruses, pro- or eucaryotic
cells) do not necessarily
precipitate or only sedimentout after they have been

exposed to higher forces than
the force of gravity resulting
from the gravitational acceleration. If these forces
exceed the counter-forces
resulting from convection(heat
circulation) and
Brownian molecular motion,
both of which cause constantmixing of solutions andsuspensions, sedimentation
takes place.

Technical appendix is subject to error and technical modifications.

Technical Appendix

The sedimentation rate canbe calculated on the basis of
Stoke‘s law as follows:V=d2 (pP – pL) g
18µInfluence of theCentrifugation
and Calculation of the RCF
respectively RPM
A centrifuge can be used
to create a transient gravitational field under the
influence of which the sedimentation of cells, cellularcomponents and macro-molecules takes place. In acentrifuge, a suspension
located in a centrifuge tube

However, a particle will only
sediment out if pP > pL.
If pP < pL, V becomes negative,
consequently the particle
floats rather than sedimenting out.

/

V = sedimentation rate
pP = density of the particle
pL = density of the liquid
g = 9.81 m / s2
µ = viscosity of the liquid/

where mP = mass of the particle,
w= angular velocity (s -1) andr = distance of the particle fromthe rotational axis/

where R = rotor revolutions
per min and r = distance of theparticle from the rotational axis
(cm)

rotates around a rotational
axis. Each particle of thesuspension is subject tocentrifugal force, whichmoves it radially awayfrom the rotational axis.
The centrifugal force FC iscalculated as follows:

The force acting on aparticle in a centrifugal
field is stated relative togravitational
acceleration,
usually as so-called relative
centrifugal force (RCF) or
g-force (x g). It is calculated
as follows:

For easier conversion of the
RCF or g-force into revolutions per min, the equation
can be transformed asfollows:

Technical appendix is subject to error and technical modifications.

FC = m P w 2 r

RCF = 11.18r

R = 299

(

√

R
1000

)

2

RCF
r

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/ 281

Technical Appendix

ALIGNMENT CHART
/

speed
(rpm)

By use of a ruler, thethird value relating to
two known scale values
can be read from thealignment chart.

Centimetre

RCF

RCF

MAXIMAL CENTRIFUGE CAPACITY OF TUBES,REACTION TUBES AND MICROPLATES
The maximum
centrifuge capacityfor Greiner Bio-One tubes,
reaction tubes
and microplates islisted in the formof the RCF in
the tables below.

For centrifugation, all products
were filled with water up to
their maximum filling volume.
Determination of the maximum
RCF in a swinging-bucket
rotor was conducted in a
Thermo Scientific Centrifuge(Heraeus Multifuge BSR

Plus). Determination of the
maximum RCF in a fixed angle
rotor was conducted in a
Sorvall Centrifuge (Evolution
RC). Therefore special rotor
inserts for different vessel
shapes and sizes were used
for a stable fit.

REACTION TUBES
Art. no.

Volume [ml]max. RCF [g] fixed-angle rotor

6162XX
6232XX
6672XX
6932XX
7162XX
7172XX
7222XX

1.5
2.0
0.5
0.5
1.5
1.5
2.0

18000
16000
15000
18000
20000
20000
22000

The stated maximum RCF values are guidelines only, depending on a variety of factors such as rotor, temperature, density, pH and type of liquid.
The suitability of tubes for a specific application using high centrifugation forces has to be tested.

282 /www.gbo.com

Technical appendix is subject to error and technical modifications.

Technical Appendix

POLYSTYRENE TUBES
Art. no.

Dimensions ø [mm] x height [mm]max. RCF [g] swinging-bucket rotor

max. RCF [g] fixed-angle rotor

1031XX
1121XX
1151XX
1161XX
1201XX
1251XX
1361XX
1601XX
1631XX
1641XX
1661XX
1861XX
1871XX
1881XX
1911XX
2011XX

10.5 x 40
12 x 55
12 x 75
12 x 75
12.4 x 75
13 x 100
14 x 100
16 x 100
17 x 10016.8 x 100
16 x 110
17 x 120
17 x 100
17 x 120
18 x 95
24 x 90

5800
5800
5800
5800
4800
4000
4000
3500
3000
5000
2500
2500
5200
2500
4000
1000

-1
6200
5800
-1
5000
7500
5200
5000
3000
5000
3200
2800
6600
4500
5500
3500

Dimensions ø [mm] x height [mm]max. RCF [g] swinging-bucket rotor

max. RCF [g] fixed-angle rotor

8.5 x 44
12 x 55
12 x 75
12.5 x 48
12.5 x 48
12.5 x 42
12.5 x 86
12.4 x 47
12.4 x 83
16 x 100
16 x 100
16 x 100
17 x 77
17 x 100
18 x 95
17 x 120
18 x 95
30 x 115
30 x 115
30 x 115
30 x 115
30 x 115
30 x 115

5800
5800
5800
5800
5800
5800
5800
5800
5800
5800
3500
5000
4800
4800
4800
4000
4800
2800
3200
3200
3200
3200
3200

20000
30000
34000
34000
34000
26000
34000
26000
34000
33000
33000
26000
34000
34000
34000
15000
34000
9500
9500
17000
17000
9500
9000

1 No fitting rotor inserts available.

POLYPROPYLENE TUBES
Art. no.

1022XX
1122XX
1152XX
1212XX
1222XX
1232XX
1242XX
1262XX
1272XX
1602XX
160297
1632XX
184261187201187261
1882XX
1912XX
2102XX
2272XX
227261227270227281 / 227285
227280 / 227283

The stated maximum RCF values are guidelines only, depending on a variety of factors such as rotor, temperature, density, pH and type of liquid.
The suitability of tubes for a specific application using high centrifugation forces has to be tested.

Technical appendix is subject to error and technical modifications.

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/ 283

Technical Appendix

POLYETHYLENE TUBES
Art. no.

Dimensions ø [mm] x height [mm]max. RCF [g] swinging-bucket rotor

max. RCF [g] fixed-angle rotor

1123XX

12 x 55

4200

22000

1153XX

12 x 75

4200

20000

1603XX

16 x 100

3500

30000

1873XX

17 x 100

5800

20000

MULTIWELL PLATES
Art. no.

Multiwell Platemax. RCF [g] swinging-bucket rotor

657160

6 well, PS, clear

4800

665102

12 well, PS, clear

4800

66216024 well, PS, clear

4800

67718048 well, PS, clear

4800

Art. no.

Microplatemax. RCF [g] swinging-bucket rotor

650101

96 well, PS, U-bottom, clear

1000

651101

96 well, PS, V-bottom, clear

1000

655101

96 well, PS, F-bottom, clear

4800

650201

96 well, PP, U-bottom, natural

4800

651201

96 well, PP, V-bottom, natural

4800

655201

96 well, PP, F-bottom, natural

4800

65520996 well, PP, U-bottom, black

4800

MICROPLATES

65507496 well, PS, F-bottom, white

4800

655076

96 well, PS, F-bottom, black

4800

65509496 well, PS, µClear®, white

4800

655096

96 well, PS, µClear®, black

4800

655801

96 well, PS, UV-Star®

4800

780201

96 well, PP, MASTERBLOCK® 1 ml

4800

78027096 well, PP, MASTERBLOCK® 2 ml

4800

786201

96 well, PP, MASTERBLOCK® 0.5 ml

4800

781101384 well, PS, clear

4800

781073384 well, PS, white

4800

781077384 well, PS, black

4800

781094384 well, PS, µClear®, white

4000

781096384 well, PS, µClear®, black

3000

781201384 well, PP, F-bottom, natural

4800

781280384 well, PP, V-bottom, natural

4800

781270384 well, PP, V-bottom, Deep Well, natural

4800

781801384 well, PS, UV-Star®

4800

The stated maximum RCF values in these tables are guidelines only, depending on a variety of factors such as rotor, temperature, density, pH and type of liquid. The suitabilityof tubes and plates for a specific application using high centrifugation forces has to be tested.

284 /www.gbo.com

Technical appendix is subject to error and technical modifications.

Technical Appendix

MICROPLATES
Art. no.

Microplatemax. RCF [g] swinging-bucket rotor

784101384 well, PS, Small Volume, clear

800

784075384 well, PS, Small Volume, white

800

784076384 well, PS, Small Volume, black

800

784201384 well, PP, Small Volume, natural

4800

7821011536 well, PS, HiBase, clear

1800

7820741536 well, PS, HiBase, white

1500

7820771536 well, PS, HiBase, black

1500

7820941536 well, PS, µClear®, HiBase, white

1000

7820961536 well, PS, µClear®, HiBase, black

1500

7822701536 well, PP, V-bottom, Deep Well, natural

4800

7831011536 well, PS, LoBase, clear

4800

7830751536 well, PS, LoBase, white

4800

7830761536 well, PS, LoBase, black

4800

7830941536 well, PS, µClear®, LoBase, white

4800

7830961536 well, PS, µClear®, LoBase, black

4800

Art. no.

PCR Platemax. RCF [g] swinging-bucket rotor

65227096 well, PP, natural, full-skirt

4800

65229096 well, PP, natural, half-skirt, suitable for ABI

4800

785201384 well, PP, natural, full-skirt

4800

785290384 well, PP, natural, full-skirt, suitable for ABI

4800

PCR PLATESFor centrifugation the plates were filled with water as follows: 96 well = 300 µl | 384 well = 50 µl | 1536 well = 5 µl
The stated maximum RCF values in these tables are guidelines only, depending on a variety of factors such as rotor, temperature, density, pH and type of liquid. The suitabilityof tubes and plates for a specific application using high centrifugation forces has to be tested.

Technical appendix is subject to error and technical modifications.

www.gbo.com

/ 285

Technical Appendix

LABORATORY INFORMATION FOR CRYO.S
SAMPLE STORAGE
FREEZING PROTOCOL

286 /

1

Wash the cells with warm PBS
solution, aspirate the solution
and cover the cells with a solution containing trypsin and EDTA
(a thin liquid film is enough;the concentration should be
evaluated for each cell line).

2

Incubate the cells for max.
3 – 5 min at 37 °C.

3Once the cells detach fromthe bottom, stop incubation
by adding cell culture medium
supplemented with serum andslightly suspend cells using apipette.

4Spin down the suspension (500 x g, 5 min) and resuspend the pellet withmedium containing serum.

5

Determine the cell number(using a Neubauer chamber).

6

Spin down the cells for 5 min
at 500 x g and discard the supernatant. Resuspend the pellet with an adequate volume ofcell culture medium containing serum.

7

Mix the cell suspension
1:1 with freezing medium(60 % medium, 20 % FCS,
20 % DMSO) and transfer itin Cryo.s. For freezing in Cryo.s,
the concentration of cells
should be 1 – 5 x 106 cells / ml.

www.gbo.com

8

9

Cryo.s containing cells shouldbe frozen at a cooling rate of-1 K / min. This can be achievedby placing them into an isopropanol-filled chamber at -70 °C.
If other types of samples arecontained, Cryo.s may be frozen directly at -20 °C, -70 °C
or in the gas phase of liquid
nitrogen. In order to assure
even freezing of the sample, 4
and 5 ml Cryo.s should be frozen at -20 °C overnight before
transferring them to -70 °C
or to the gas phase of liquid
nitrogen.
Then transfer the Cryo.s
into the nitrogen tank.
To avoid contamination (e. g.
mycoplasma) and due to safety precautions Cryo.s must
only be stored in the gas phase
above and not in the liquid
nitrogen.

Technical appendix is subject to error and technical modifications.

Technical Appendix

THAWING PROTOCOL

1

Immediately after removing
them out of the nitrogen tankthe frozen cells are thawed inabout 1 – 2 min brandishing the
Cryo.s in a water bath at 37 °C.
The thawing process should be
performed as fast as possible.

2

Transfer the thawed cellsuspension into a 15 ml tube
and mix it immediately
with copious amounts of cell
culture medium containing
serum.

3

After spinning down the cells(500 x g, 5 min) discard thesupernatant and resuspend
the pellet in an appropriate cell
culture medium supplemented
with serum and transfer it into
one or more cell culture flasks.

4

Follow the recommended cellconcentration for seeding.

5

During the next 12 hours cells
should rest.

6

A change of medium is recommended after 24 resp. 48 hours.

SAFETY ADVISORY FOR
WORKING WITH CRYO.S
Cryo.s, tubes are intended for sample storage
exclusively in the gas phase over liquid
nitrogen or in freezers! If Cryo.s are stored
in the liquid phase, nitrogen can seep into
the tubes. Then upon thawing the vaporising
nitrogen can generate high pressure, ultimately resulting in an explosion, as well as therelease of any infectious material.
Always take appropriate personal safety measures when working with Cryo.s, including
wearing safety clothing, using goggles

Technical appendix is subject to error and technical modifications.

and working at a safety laboratory bench.
When undertaking cryogenic preservation,
Cryo.s must be evenly exposed to freezing
temperatures. Uneven temperature exposures can cause formation of ice plugs (i. e.
at tube top) that inhibit the expansion offreezing liquid (i. e. at tube bottom), resulting
in dangerous high pressure and subsequent
harm or damage of tubes.
Never exceed maximum working volumes as
specified.

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/ 287

Technical Appendix

LABORATORY INFORMATION FOR IMMUNOLOGYVOLUME-DEPENDENT WETTING OF IMMUNOLOGICAL PRODUCTS
Liquid volume[µl]

Covered area
[mm2]

Liquid height[mm]

Area / volume
ratio [cm2 / cm3]

96 Well ELISA Microplate, U-Bottom
25
50
75
100
125
150
175
200
225
250
275
300

34
52
68
84.6
99
115.5
130
145
160
174.7
190
205

1.7
2.6
3.4
4.2
4.9
5.7
6.4
7.1
7.8
8.5
9.2
9.9

35
54.6
72.4
88.6
105
123.8
140.8
156

2.3
3.4
4.4
5.3
6.2
7.2
8.1
8.9

14.0
10.9
9.7
8.9
8.4
8.3
8.0
7.8

25
50
75
100
125
150
175
200

47
62
77.5
92
108
123
137.6
152.3
168
183
197
212

0.8
1.55
2.3
3.0
3.8
4.5
5.2
5.9
6.65
7.35
8.0
8.7

288 /

www.gbo.com

47
64
78.5
93
108
122.6
137.5
152
167
182
197
212

0.7
1.5
2.2
2.9
3.6
4.3
5.0
5.7
6.4
7.1
7.8
8.4

Area / volume
ratio [cm2 / cm3]

18.8
12.4
10.3
9.2
8.6
8.2
7.9
7.6
7.5
7.3
7.2
7.1
18.8
12.8
10.5
9.3
8.6
8.2
7.9
7.6
7.4
7.3
7.2
7.1

38
60
81.5
103.6
124.5
144
165.8
181.7

1.65
3.2
4.7
6.2
7.6
8.9
10.3
11.5

15.2
12.0
10.9
10.4
10.0
9.6
9.5
9.1

39
56
73
88.6
104.3
120
136.5
150.8
165.4
181
196
211

1.0
1.9
2.8
3.6
4.4
5.2
6.0
6.7
7.4
8.15
8.85
9.55

15.6
11.2
9.7
8.9
8.3
8.0
7.8
7.5
7.4
7.2
7.1
7.0

F8 Strip Plate
25
50
75
100
125
150
175
200
225
250
275
300

96 Well ELISA Microplate, F-Bottom / Chimney Well
25
50
75
100
125
150
175
200
225
250
275
300

Liquid height[mm]

C8 Strip Plate
25
50
75
100
125
150
175
200
225
250
275
300

96 Well ELISA Microplate, F-Bottom / Standard
25
50
75
100
125
150
175
200
225
250
275
300

Covered area
[mm2]

96 Well ELISA Microplate, F-Bottom, Half Area
13.6
10.4
9.1
8.5
7.9
7.7
7.4
7.3
7.1
7.0
6.9
6.8

96 Well ELISA Microplate, V-Bottom
25
50
75
100
125
150
175
200

Liquid volume
[µl]

50.4
64
79.7
93.5
108.3
123
138
153
167
182
196
211.5

0.8
1.45
2.2
2.85
3.55
4.25
4.95
5.65
6.3
7.0
7.65
8.35

20.2
12.8
10.6
9.4
8.7
8.2
7.9
7.7
7.4
7.3
7.1
7.1

U8 Strip Plate
25
50
75
100
125
150
175
200
225
250
275
300

34
52
68
84
99.6
115
129.6
144
159
174
189
204

1.7
2.6
3.4
4.2
4.95
5.75
6.45
7.15
7.85
8.55
9.25
9.95

13.6
10.4
9.1
8.4
8.0
7.7
7.4
7.2
7.1
7.0
6.9
6.8

Technical appendix is subject to error and technical modifications.

Technical Appendix

Liquid volume[µl]

Covered area
[mm2]

Liquid height[mm]

Area / volume
ratio [cm2 / cm3]

Liquid volume[µl]

U16 Strip Plate
25
50
75
100
125
150
175
200
225
250
275
300

35
52
68
84
98.6
115
129.6
144
159
174
189
204

1.75
2.6
3.4
4.2
4.9
5.7
6.4
7.1
7.8
8.5
9.2
9.9

Covered area
[mm2]

Liquid height[mm]

Area / volume
ratio [cm2 / cm3]

F16 Strip Plate
25
50
75
100
125
150
175
200
225
250
275
300

14.0
10.4
9.1
8.4
7.9
7.7
7.4
7.2
7.1
7.0
6.9
6.8

49
63
79.8
94.3
108
123.5
138
153
168
183
198
213

0.8
1.5
2.3
3.0
3.7
4.4
5.1
5.8
6.5
7.2
7.9
8.6

19.6
12.6
10.6
9.4
8.6
8.2
7.9
7.7
7.5
7.3
7.2
7.1

384 Well Microplate, F-Bottom
25
50
75
100
125
132

39.07
66.60
94.03
119.63
145.6
152.6

2.50
4.8
7.00
9.05
11.05
11.50

15.6
13.3
12.5
12.0
11.6
11.6

Abbreviations
ANSI
COC
COP
CV
DMSO
DNA
DNase
ECM
EL-Rack
ELISA
EVA
FDA
FIA
F-Rack
HDPE
HLA
HTS
IgG
ID-Card
LAL
LIA
med.
NMWCO
PC
PCR
PDL
PET
PETG
pH
PLA

American National Standards Institute
Cycloolefin co-polymer
Cycloolefin polymer
Coefficient of Variation
Dimethyl Sulphoxide
Deoxyribonucleic Acid
Deoxyribonuclease
Extracellular Matrix
EasyLoad® Rack
Enzyme Linked Immuno Sorbent Assay
Ethyl Vinyl Acetate
Food and Drug Administration
Fluorescence Immuno Assay
Filter Tip RackHigh Density Polyethylene
Human Leucocyte Antigen
High-Throughput Screening
Immunoglobulin G
Identity Card
Limulus Amoebocyte Lysate
Luminescence Immuno Assay
MediumNominal Molecular Weight Cut-Offs
Polycarbonate
Polymerase Chain Reaction
Poly-D-Lysine
Polyethylene Terephthalate
Polyethylene Terephthalate Copolymer
pH Value
Polylactate

Technical appendix is subject to error and technical modifications.

PLL
Poly-L-Lysine
PP
Polypropylene
PS
Polystyrene
PTFE
Polytetrafluoroethylene
RNA
Ribonucleic Acid
RNase
RibonucleaserRNA
Ribosomal RNA
RT
Room Temperature
SBS
Society for Biomolecular Sciences
SPA
Scintillation Proximity Assays
ST-Rack
Standard RackTC
Tissue Culture
USP
United States Pharmacopoeia
UV Spectrum Ultraviolet Spectrum
VIS Spectrum Visible Spectrum
Units
°C
Da
g
Gy
h
l
M
m
min
Mol
s

Degree Centigrade
Dalton, the unit of molecular mass
Gram or Gravitational
Acceleration (about 9.81 m/s2)
Gray, Radiation Unit
Hour
Liter
Molarity, moles of solute
per litre of solution
Meter
MinuteAbsolute Amount of Substance
Secondwww.gbo.com

/ 289

Technical Appendix

GLOSSARY
Advanced TC is a polymer modification increasing thecellular primary and long-term adhesion of Greiner
Bio-One cell culture vessels. Based on the innovativetechnique the surface of the cell culture vessels is
modified to positively influence cellular features andfunctions. Enhanced cellular adhesion and higher
proliferation rates improve cell expansion and cultivation of sensitive cells or cells under restricted growth
conditions.
Biobanking Tubes are 300 µl, 600 µl and 1000 µl Cryo.s
tubes for the efficient storage of biological samples inlarge-scale biorepositories. The design of tubes andracks allows for a very space-efficient storage with up
to 30 % better utilisation of storage space in freezers
or liquid nitrogen tanks. In addition, they are optimised
for sample storage at extremely low temperatures overlong periods of time.
Bioburden is used to describe the colonisation of viablemicroorganisms on a material or product and is thebasis for determining the necessary radiation dose forsterilisation.
C-bottom stands for the well profile of a flat well bottom
with rounded corners.
CELLCOAT® is the Greiner Bio-One brand name for all protein-coated cell culture vessels for adherent cell culture.
CELLMASTER is a quality term that refers to all roller
bottles.
CELLreactor is a 15 ml / 50 ml polypropylene tube withfilter screw cap for the cultivation of suspension andspheroid cells, expansion of aerobic bacteria, yeast orother microorganisms as well as storage of components
and liquids requiring gas exchange.

F-bottom stands for a flat bottom well profile.
F-bottom / chimney well stands for the well profile ofa flat well bottom in a chimney-like arrangement. In
other words, each well stands on its own. The risk ofcontamination from sample material being carried overis minimised.
FLUOTRAC is a quality term for immunological products, referring to black microplates (fluorescencemeasurement).
FourWell Plate is a subdivided plate for microscopic
applications facilitating the cultivation of cells andthe storage of microscopic slides in an HTS-compatible
plate complying with ANSI standards.
Hanging Drop is a technique for protein crystallisation
based on › vapour diffusion, where droplets literally
hang from the top of an upper substrate.
HiBase is a special plate profile of 384 well › Small Volume
and 1536 well microplates. In contrast to the › LoBase
profile, the HiBase profile is particularly well suited for
top-reading systems, since the measuring optic has aminimal separation from the upper edge of the well inthis plate profile.
High binding microplates (= MICROLON 600, FLUOTRAC
600 and LUMITRAC 600) are immunological microplateswith a high-binding polystyrene surface. Hydrophilicgroups are introduced to the polystyrene surface byphysical treatment. The high binding surface contains
more hydrophilic groups than the less hydrophilic› medium binding surface.

Cell-repellent surface reliably prevents cell attachment
in suspension cultures of semi-adherent and adherent
cell lines where standard hydrophobic surfaces generally used for suspension culture are insufficient.

LoBase is a special plate profile in 1536 well microplates.
In contrast to the › HiBase profile, the LoBase profile
is particularly well suited for bottom-reading sytems,
since the measuring optic has a minimal separation fromthe well bottom in this plate profile.

CELLSTAR® is a Greiner Bio-One brand name and includes culture vessels with physically modified surfaces
for adherent or suspension cell cultures.

LUMITRAC is a quality term for immunological products, referring to white microplates (luminescencemeasurement).

CELLview is a quality term for cell culture productswith glass bottom for high-resolution microscopic
applications.

MASTERBLOCK® is a brand name that stands for polypropylene microplates that are suitable for the storage of
non-human sample material. They are also ideally suitedfor cultivating bacteria or yeast.

Datamatrix Code is a 2D barcode which can also be used
for tracking biological and medical reagents andsamples. Its small footprint provides nearly infinite
scalability and large data capacity. Datamatrix codes
can be scanned independent of their orientation andare very accurate due to the Reed-Solomon error
correction method.
Deep Well microplates have conical bottom wells and are
ideally suited for the storage of non-human samples.
EASYstrainer are cell strainers for the fast and safe
filtration of cell suspensions such as those from tissue

290 /dissociation or for flow cytometry.

www.gbo.com

Med.
binding(medium
binding)
microplates(= MICROLON 200, FLUOTRAC 200 and LUMITRAC 200)
are immunological microplates with a less hydrophobic
surface than › high binding microplates.
Microbatch under oil is a method for protein crystallisation where the droplet is covered with oil. The oil generally
used is paraffin wax and/or silicone oil. Paraffin wax allows
little to no diffusion of water out of the droplet. Hence, all
the reagents involved in the crystallisation process, aswell as the protein, are present at defined concentrations,and no significant increase of concentration occurs

Technical appendix is subject to error and technical modifications.

Technical Appendix

within the crystallisation droplet. When paraffin wax ismixed with silicone oil, it is possible for water to diffuse
out of the droplet through the oil and both protein andreagent concentrations increase within the droplet.
µClear® (Micro-Clear) microplates, in contrast to standard microplates with a solid bottom, have a very thin foil
bottom. µClear® microplates are ideal for cell-based test
systems, microscopic analyses, as well as for bottomreading systems.
MICROLON is a quality term for immunologicalproducts, referring to clear microplates (transmission
measurement).
Non-binding microplates are characterised by low
protein, DNA, RNA and peptide binding properties.
OneWell Plate is a non-divided HTS plate for tissue
culture applications complying with the ANSI standards.
The plate is also available in a non-TC-treated version forbacteriology.

In contrast to the 384 well standard microplate, thesample volume can be considerably reduced, while thedetection limit remains the same or is even improved.
TC surface treatment stands for a special physicalprocedure with which the surfaces of CELLSTAR®
products for adherent cell culture are treated.
This treatment leads to the incorporation of polargroups, such as carboxy and hydroxy groups,
into the plastic surface making it hydrophilic.
This enables the adhesion of cells to the plastic surface.
U-bottom stands for well profile of wells withround bottom.
UV-Star® microplates are made of polyolefin and have afilm bottom. In contrast to standard microplates witha solid bottom, they are characterised by an extended
transparency range to as low as 200 nm.

Sitting Drop is a technique for protein crystallisation
based on › vapour diffusion, where droplets sit on thebottom of a substrate.

Vapour diffusion is the most commonly used method forprotein crystallisation. In this method a crystallisation
droplet, formed by combining a protein solution with areagent solution, is incubated together with a larger
volume of the same reagent solution within a closed
system. The reagent solution can contain a wide
range of chemicals, e. g. buffers, salts or precipitating
agents. Due to mixing the reagent and protein solutions,the concentration of reagents within the crystallisation
droplet becomes lower than the concentration of thereagent solution itself. This causes water to evaporateout of the droplet until equilibrium is reached. During
this process, the concentration of protein and chemicals
in the crystallisation droplet is continuously rising, and,if optimal conditions have been chosen, protein crystals
will begin to form. Vapour diffusion experiments
are most often set up as › hanging or › sitting drop.

Small Volume is a well profile that was developedin 384 well format for reducing the sample volume.

V-bottom stands for the well profile of wells with a conically tapered well bottom.

Sapphire is a quality term for pipette and filter tips. The
product family comprises standard pipette tips, standard
filter tips as well as a low-retention version of both.
All tips are transparent, graduated and allow precise
pipetting with maximal recovery. They can be used withall common pipettes.
SCREENSTAR is a quality term for microplates manufactured out of high-quality cycloolefin with an ultra-clear
film bottom for high-content and high-throughput
screening.

GLOSSARY OF SYMBOLS
Manufacturer

Keep dryUse-by dateIn vitro diagnosticIn
vitro diagnostic
medicaldevicemedical device

CautionCautionThis way up

Batch code

Do not re-use

Fragile, handle with care

ArticlenumberCatalogue
numberConsult instructions for use

Temperature limit

Sterilised by irradiation

Do not use if package is damaged

Technical appendix is subject to error and technical modifications.

www.gbo.com

/ 291

Numerical Index

NUMERICAL INDEX
102201������������ 182
102261������������ 182
102270����������� 182
112101�������������� 181
112201������������� 181
115001������������ 166
115061������������� 166
115070������������ 166
115071������������� 166
115101�������������� 181
115201�������������� 181
115261������������� 187
115262������������ 187
120160��������������73
120161������������� 187
120180������������ 187
120190��������������73
121261������������� 198
121263������������ 198
121277������������ 198
121278������������ 198
121279������������ 198
121280������������ 198
122261������������ 198
122263����������� 198
122263-2D3����203
122263-2DG���203
122263-TRI���� 198
122277����������� 198
122278����������� 198
122279����������� 198
122280����������� 198
123261������������ 197
123263����������� 197
123263-2D3����203
123263-2DG���203
123263-TRI����� 197
123277������������ 197
123278������������ 197
123279������������ 197
123280����������� 197
124261����������� 200
124263����������� 200
124273����������� 200
124274����������� 200
124275����������� 200
124276����������� 200
126261������������ 198
126263����������� 198
126263-2D1����203
126263-2DG���203
126263-TRI����� 198
126277������������ 199
126278������������ 199
126279����������� 199
126280����������� 199
127261������������ 199
127263����������� 199
127263-2D1��� 204
127263-2DG���203
127277������������ 199
127278������������ 199
127279������������ 199
127280����������� 199
131202������������207
131263������������206292 /www.gbo.com

132202���������� 209
132263�����������208
133202������������211
133263����������� 210
136101�������������� 181
160101������������� 181
160201������������� 181
163160��������������73
163177������������ 186
163288����������� 189
163289����������� 189
163290����������� 189
164160��������������73
164161������������� 186
164180������������ 186
169101�������������� 181
186171������������� 187
187101�������������� 181
187201������������� 181
187261������������ 187
187262����������� 187
188161������������� 186
188171������������� 187
188241��������������73
188241������������ 186
188261-N�������� 184
188271-N�������� 184
188271-TRI����� 184
188280����������� 184
188281������������ 184
188283����������� 184
188285����������� 184
191160���������������73
191161������������� 187
191180������������� 187
201150������������� 191
201152������������� 191
201170������������� 191
201172������������� 191
205101������������ 192
210261������������ 185
210270����������� 185
217101������������� 192
224170������������� 191
225170������������ 191
225180������������ 191
227245�������������73
227245����������� 186
227250����������� 189
227255����������� 189
227261����������� 185
227261-N������� 185
227261-TRI����� 185
227270����������� 185
227270-N������� 185
227280����������� 185
227281����������� 185
227283����������� 185
227285����������� 185
227288����������� 189
227289����������� 189
227290����������� 189
303321����������� 182
310321������������ 182
330070���������� 193
332070����������� 193

354070����������� 193
366380���������� 220
366383���������� 220
366384���������� 220
366385���������� 220
366386���������� 220
373250�����������229
373270�����������229
373273�����������229
373274�����������229
373275�����������229
373276�����������229
373277�����������229
373281������������229
381061������������ 150
381070����������� 150
381080����������� 150
381081������������ 150
385270����������� 212
385273����������� 212
385274����������� 212
385275����������� 212
385276����������� 212
385277����������� 212
385278����������� 212
385279����������� 212
420161������������ 175
420180����������� 175
421084����������� 175
421161������������� 175
421180������������ 175
501870����������� 152
503850���������� 153
503870����������� 152
541070�������������45
541080�������������45
542000������������75
542040������������75
542070�������������75
542100�������������75
542120�������������75
542140�������������75
542170�������������75
543078������������ 113
543079������������ 113
543978������������ 113
543979������������ 113
604107����������� 247
604160����������� 247
604160-TRI����248
604181������������ 247
606107����������� 247
606160����������� 247
606160-TRI����248
606180����������� 247
606190�����������248
607107����������� 247
607160����������� 247
607160-TRI����248
607180����������� 247
607190�����������248
608281�����������228
609101������������ 152
609120����������� 152
609130����������� 152
609171������������ 152

609180����������� 152
609801����������� 152
609820���������� 152
609830���������� 152
609871����������� 152
612301������������249
612361������������249
612362�����������249
613101������������ 222
614101������������ 222
616201������������ 219
616283����������� 219
622201���������� 220
622261���������� 220
623201����������� 219
627102����������� 173
627160�������������49
627170�������������49
627860������������� 51
627860�������������111
627861�������������� 51
627861��������������111
627870�������������52
627870�������������111
627871�������������� 51
627871��������������111
627960�������������49
627965������������� 51
627965�������������111
627975�������������52
627975�������������111
627979������������� 51
627979������������� 91
628102����������� 173
628160�������������49
628160-TRI������49
628161������������ 173
628910�������������50
628920�������������50
628930�������������50
628940�������������50
628950�������������50
628960�������������49
628979������������� 51
628979������������� 91
629161������������ 173
629180������������174
632180����������� 173
633180����������� 173
633181������������ 173
635102������������174
639102����������� 173
639160�������������49
639160-TRI�������49
639161������������ 173
639960�������������50
643201������������� 47
643201����������� 176
643203������������� 47
643203����������� 176
643401������������� 47
643401����������� 176
644201������������� 47
644201����������� 176
644203������������ 47
644203���������� 176

644401������������� 47
644401����������� 176
646201������������� 47
646201����������� 176
646203������������� 47
646203����������� 176
646401������������� 47
646401����������� 176
649201������������� 47
649201����������� 176
649203������������� 47
649203����������� 176
649401������������� 47
649401����������� 176
650001������������ 161
650061������������ 161
650061������������ 161
650101������������ 127
650160�������������65
650161������������ 127
650180�������������65
650185�������������68
650201����������� 129
650209���������� 129
650261����������� 129
650901����������� 142
650970�������������92
650979�������������92
651001������������� 161
651061������������� 161
651101������������� 127
651160��������������65
651161������������� 127
651180��������������65
651201������������ 129
651209����������� 129
651524��������������97
651596��������������98
651901������������ 142
651970�������������92
652201����������� 231
652210����������� 231
652250����������� 231
652260����������� 231
652270����������� 231
652290����������� 231
653180����������� 168
653190����������� 168
654180����������� 168
655001������������ 161
655061������������ 161
655073�������������65
655074����������� 127
655075����������� 127
655076����������� 127
655077����������� 127
655079�������������65
655080����������� 161
655081������������ 161
655083�������������65
655086�������������65
655087�������������66
655088�������������66
655090������������66
655094����������� 127
655095����������� 127

Numerical Index

655096����������� 127
655097����������� 127
655098�������������66
655101������������ 127
655160�������������65
655161������������ 127
655161������������ 127
655162�������������65
655167��������������63
655169��������������63
655180�������������65
655182�������������65
655185��������������68
655201����������� 129
655209����������� 129
655630�������������63
655640�������������63
655641�������������63
655680�������������63
655801������������147
655830�������������95
655840�������������95
655841�������������95
655846�������������97
655850�������������95
655866������������ 117
655891������������ 115
655892������������ 119
655900���������� 142
655901����������� 142
655903����������� 142
655904����������� 142
655906����������� 142
655930�������������67655936�������������67655940�������������67655944�������������67655946�������������67655948�������������67
655950�������������67
655956�������������67655970�������������92
655976�������������92
655976-SIN������92
655980�������������68
655981������������ 115
655983�������������68
655986�������������68
655990����������� 145
655994����������� 145
655997����������� 145
656101�������������� 71
656101������������ 149
656161�������������� 71
656161������������ 149
656170������������� 71
656170����������� 149
656171�������������� 71
656171������������ 149
656190������������� 71
656190����������� 149
656191�������������� 71
656191������������ 149
657102������������174
657110��������������60
657110������������ 102
657160�������������54
657185��������������54
657610�������������58
657610����������� 100
657630�������������58
657630����������� 100

657631��������������58
657631������������ 100
657638�������������58
657638����������� 100
657640�������������58
657640����������� 100
657641��������������58
657641������������ 100
657824�������������97
657825�������������96
657830�������������96
657840�������������96
657841��������������98
657843�������������98
657846�������������98
657850�������������97
657852�������������98
657896�������������97
657930�������������55
657940�������������55
657950�������������55
657960�������������54
657970�������������56
657970�������������92
658170������������� 41
658170-TRI������� 41
658175�������������� 41
658175-TRI������� 41
658190�������������43
658190-TRI������43
658195�������������43
658195-TRI�������43
658910�������������43
658920�������������43
658940�������������42658950�������������42658970�������������42658975�������������42658980������������ 44
658980������������� 91
658985������������ 44
658985������������� 91
659180����������� 168
659190����������� 168
660160������������� 41
660160-TRI������ 41
660175������������� 41
660175-TRI������� 41
660190�������������43
660190-TRI������43
660960������������42
660975�������������42
660980����������� 44
660980������������ 91
660985������������ 44
660985������������� 91
661160�������������� 41
661175�������������� 41
661190��������������43
661195��������������43
661195-TRI�������43
661910��������������43
661920�������������43
661940�������������43
661950�������������42661980������������ 44
661980�������������92
661985������������ 44
661985�������������92
662102�������������54
662145����������� 152
662150����������� 152

662160�������������54
662610�������������60
662610����������� 102
662630�������������60
662630����������� 102
662631�������������60
662631����������� 102
662638�������������60
662638����������� 102
662640�������������60
662640����������� 102
662641�������������60
662641����������� 102
662825�������������96
662830�������������96
662840�������������96
662892������������ 119
662930�������������55
662940�������������55
662950�������������55
662960�������������55
662970�������������56
662970�������������92
664102����������� 173
664160�������������49
664160-TRI�������49
664161������������ 173
664910�������������50
664920�������������50
664940������������50
664950�������������50
664960�������������50
664970������������� 51
664970������������� 91
665102�������������54
665110�������������� 61
665110������������ 103
665180�������������54
665610�������������59
665610������������101
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665631������������101
665638�������������59
665638������������101
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665640������������101
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665641������������101
665970�������������92
665980�������������54
667201����������� 219
668102���������� 220
669285����������� 231
670102������������174
670180�������������52
670190�������������52
671201������������227
671221������������228
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673275�����������228
673276�����������228

673277�����������228
673281������������228
673283�����������228
675001������������ 161
675061������������ 161
675074����������� 128
675075����������� 128
675076����������� 128
675077����������� 128
675083�������������66
675086�������������66
675090�������������67675096����������� 128
675101������������ 128
675161������������ 128
675180�������������66
675801������������147
676001����������� 149
676040����������� 149
676050����������� 149
676051����������� 150
676070����������� 149
676090����������� 149
677102�������������54
677180�������������54
677970�������������56
677970�������������92
677980�������������55
678101��������������82
678101-CF1�������84
678101-EXF������83
678104�������������82
678104-CF1������84
678104-CF2�����84
678104-EXF�����83
678108�������������82
678108-CF1������84
678108-CF2�����84
678108-EXF�����83
678112��������������82
678112-CF1�������84
678112-CF2������84
678112-EXF������83
678116��������������82
678116-CF1�������84
678116-CF2������84
678116-EXF������83
678124��������������82
678124-CF1������84678124-CF2������84678124-EXF�����83
678140�������������82
678140-CF1������84678140-CF2�����84678140-EXF�����83
678904�������������82
678908�������������82
678912��������������82
678916��������������82
678924�������������82
678940�������������82
680190������������ 80
680195������������ 80
680645�������������79
680648������������79
680658�������������79
680660������������79
680665�������������79
680668�������������79
681670�������������79
681672�������������79
681675��������������79

682201�����������227
682273�����������227
682274�����������227
682275�����������228
682276�����������228
682277�����������228
682281�����������228
682612�������������79682615�������������79
682624�������������79
682625�������������79
682660�������������79682670�������������79682672�������������79682678�������������79
683201�����������227
683271�����������227
683273�����������227
683274�����������227
683275�����������227
683276�����������227
683277�����������227
684201�����������228
688102������������174
690160������������� 41
690160-TRI������ 41
690170������������� 41
690175������������� 41
690175-TRI������� 41
690190�������������43
690190-TRI������43
690195�������������43
690195-TRI�������43
690910�������������43
690920������������43
690940������������42
690950������������42
690975�������������42
690980����������� 44
690980������������ 91
690985������������ 44
690985������������� 91
691101��������������� 71
691101������������� 149
691161��������������� 71
691161������������� 149
700361�����������249
700370�����������249
705063����������� 164
705065����������� 164
705066����������� 164
705070����������� 164
705071����������� 164
705073����������� 164
705074����������� 164
705075����������� 164
705076����������� 164
710107������������ 247
710160������������ 247
710160-TRI�����248
710180������������ 247
710183������������248
716201������������ 219
717201������������ 219
722201����������� 219
724410������������� 191
724411������������� 191
724412������������� 191
724413������������� 191
724414������������ 192
725201����������� 219
725261������������ 219

www.gbo.com

/ 293

Numerical Index

729101����������� 220
731101������������� 175
731161������������� 175
731165������������ 175
731170������������ 175
731171������������� 175
731175������������ 175
731180������������ 176
731181������������� 176
731185������������ 176
745290�����������245
754061����������� 163
754070����������� 163
756070����������� 163
756071����������� 163
760107����������� 247
760160����������� 247
760160-TRI����248
760180����������� 247
762070����������� 163
762071����������� 163
762074����������� 163
762075����������� 163
762076����������� 163
762077����������� 163
767070����������� 163
767071������������ 163
768160����������� 247
768160-TRI�����248
768180����������� 247
769190����������� 168
770310�����������244
770320�����������244
770330�����������244
770340�����������244
770370�����������244
770380�����������244
771350�����������238
771351������������238
771352������������238
771353������������238
771354������������238
771355������������238
771361������������238
771362������������238
771363������������238
771364�����������238
771365������������238
772350�����������239
772351������������239
772352�����������239
772353�����������239
772354�����������239
772355�����������239
772361������������239
772362�����������239
772363�����������239
772364�����������239
772365�����������239
773353�����������240
773363�����������240
774353�����������240
774363�����������240
775350������������241
775351�������������241
775352������������241
775353������������241
775354������������241
775355������������241
775361�������������241
775362������������241
775363������������241294 /www.gbo.com

775364������������241
775365������������241
775390�����������245
776350�����������242
776351������������242
776352�����������242
776353�����������242
776354�����������242
776355�����������242
776361������������242
776362�����������242
776363�����������242
776364�����������242
776365�����������242
777350�����������243
777351������������243
777352�����������243
777354�����������243
777355�����������243
777361������������243
777362�����������243
777364�����������243
777365�����������243
778350�����������243
778351������������244
778352�����������244
778353�����������244
778354�����������244
778355�����������244
778361������������244
778362�����������244
778363�����������244
778364�����������244
778365�����������244
779160������������ 44
779190�������������45
780201����������� 137
780215����������� 137
780261����������� 137
780270����������� 137
780271����������� 137
780285����������� 137
781061������������� 131
781073��������������69
781074������������� 131
781075������������� 131
781076������������� 131
781077������������� 131
781079��������������69
781080�������������69
781086�������������69
781090�������������69
781091��������������69
781092�������������69
781093�������������69
781095������������ 131
781096������������ 131
781097������������� 131
781098�������������69
781101�������������� 131
781162������������� 131
781165��������������69
781182��������������69
781185������������� 131
781186������������� 131
781201������������ 132
781201-906���� 132
781201-906���� 140
781209����������� 133
781270������������ 138
781271������������ 138
781280����������� 133

781801�������������147
781830�������������96
781840�������������95
781841��������������96
781846��������������97
781850�������������96
781866������������� 117
781892������������� 119
781900����������� 142
781901������������ 142
781903����������� 142
781904����������� 142
781906����������� 142
781930�������������69
781936��������������69
781940�������������69
781944�������������69
781946��������������69
781948�������������69
781950�������������69
781956��������������69
781970��������������92
781974��������������92
781976��������������92
781976-SIN������93
781983��������������69
781986��������������69
781990����������� 145
781995������������ 145
781997������������ 145
782061����������� 135
782073�������������70
782074����������� 135
782075����������� 135
782076����������� 135
782077����������� 135
782078�������������70
782080�������������70
782086�������������70
782092�������������70
782093�������������70
782095����������� 135
782096����������� 135
782097����������� 135
782101������������ 135
782180�������������70
782261����������� 138
782270����������� 138
782855����������� 140
782900����������� 143
782904����������� 143
782946�������������70
782974�������������93
783892������������ 119
784075����������� 132
784075-25����� 132
784076����������� 132
784076-25����� 132
784080�������������70
784086�������������70
784101������������ 132
784201����������� 132
784900����������� 143
784904����������� 143
784946�������������70
785285����������� 231
785290����������� 231
786201����������� 137
786261������������ 137
787979�������������93
788876������������147
789866������������ 117

789979�������������93
792870-906��� 140
793855����������� 140
802202���������� 201
802203���������� 201
802204���������� 201
802206���������� 201
802225���������� 201
802501���������� 200
802506��������� 204
802576���������� 204
803202��������� 204
803270���������� 204
843070����������253
844070����������254
845070�����������254
846070����������253
847070�����������249
848070����������255
848902����������255
848913�����������255
848915�����������256
848916�����������255
848921�����������255
848923�����������255
848950����������256
849050���������� 214
849070���������� 214
852076-AU����� 213
852076-CN���� 213
852076-EU���� 213
852076-JP����� 213
852076-NA���� 213
852076-UK����� 213
852078-AU����� 213
852078-CN���� 213
852078-EU���� 213
852078-JP����� 213
852078-NA���� 213
852078-UK����� 213
878071�������������86878072�������������85878073�������������85878074�������������85878075�������������86
950700������������46
951700�������������46
952700�������������46
960161������������ 192
960177����������� 192
968177������������ 192
975502����������� 137
975570����������� 137
976501����������� 212
976561������������206
976563�����������206
976564�����������206
976565�����������206
976566�����������206
976567�����������206
976568�����������206
976569�����������206
976570�����������207
976580�����������207
976583�����������207
976584�����������207
976585�����������207
976586�����������207
976587�����������207
976588�����������207
976589�����������207
977501����������� 212

977561������������208
977563�����������208
977564�����������208
977565�����������208
977566�����������208
977567�����������208
977568�����������208
977569�����������208
977570���������� 209
977580���������� 209
977583���������� 209
977584���������� 209
977585���������� 209
977586���������� 209
977587���������� 209
977588���������� 209
977589���������� 209
978501����������� 213
978561������������ 210
978563����������� 210
978564����������� 210
978565����������� 210
978566����������� 210
978567����������� 210
978568����������� 210
978569����������� 210
978570������������211
978580������������211
978583������������211
978584������������211
978585������������211
978586������������211
978587������������211
978588������������211
978589������������211
89000002������235
89000010�������235
89000020������235
89000099������236
89000100�������235
89000200������235
89000500������235
89000810�������235
89000820������235
89001000�������235
89001210��������236
89001220�������236
89008200������236
89008300������236
89010000�������235
89012200�������236
89012300�������236
96077307���������52

Glossary

GLOSSARY
1536 Well Microplates������������������������������������������������� 135,138,140,142
2 ml aspiration pipette������������������������������������������������������������������248
24 Well ComboPlate���������������������������������������������������������������� 152,153
384 Well Microplates���������������������������������131,132,138,140,142,145,147
384 Well PCR Microplates������������������������������������������������������������� 231
3D Cell Culture������������������������������������������������������������� 91,95,96,97,98
48-way Datamatrix Cryo Rack������������������������������������������������������204
81-way Datamatrix Cryo Rack�������������������������������������������������������204
96 Well ELISA Microplates������������������������������������������������������������� 161
96 Well ELISA Strip Plates������������������������������������������������������ 163,164
96 Well Microplates������������������������������������ 127,128,129,137,142,145,147
96 Well PCR Microplates��������������������������������������������������������������� 231
96 Well Storage Box�����������������������������������������������������������������������137
96-way Datamatrix Cryo Rack������������������������������������������������������ 212
Advanced TC������������������������������������������������������������������������������54,55
Analyser Cups�������������������������������������������������������������������������������220
AutoFlask - Cell Culture Flask����������������������������������������������������44,45
Bacteriology����������������������������������������������������������������������173,174,175
Biobanking Tubes������������������������������������������������������������������������� 212
CELLCOAT®�������������������������������������������������������������������������������42,55
CELLdisc������������������������������������������������������������������������������82,83,84
CELLevator������������������������������������������������������������������������������������86
CELLhandle������������������������������������������������������������������������������������85
CELLreactor����������������������������������������������������������������������������� 73,186
CELLring����������������������������������������������������������������������������������������86
CELLstage��������������������������������������������������������������������������������������85
CELLview Dish�����������������������������������������������������������������������51,52,111
CELLview Plate������������������������������������������������������������������������������ 115
CELLview Slide������������������������������������������������������������������������������ 113
CapMats���������������������������������������������������������������������������������������� 150
Ceaprene stopper��������������������������������������������������������������������������193
Cell Culture�������������������������������������������������������34,35,36,37,38,39,124
Cell Culture Dishes�������������������������������������������������������������������������49
Cell Culture Dishes - Advanced TC���������������������������������������������49,50
Cell Culture Dishes - CELLCOAT®���������������������������������������������������50
Cell Culture Dishes - Cell-Repellent Surface������������������������������ 51,91
Cell Culture Flasks������������������������������������������������������� 40,41,44,45,91
Cell Culture Flasks - Advanced TC��������������������������������������������������42
Cell Culture Flasks - CELLCOAT®����������������������������������������������������42
Cell Culture Inserts���������������������������������������������58,59,60,100,101,102
Cell Culture Microplates������������������������������������������������������� 69,70,92
Cell Culture Multiwell Plates������������������������������������������������� 54,55,56
Cell Culture Roller Bottles���������������������������������������������������� 78,79,80
Cell Culture Tubes��������������������������������������������������������������������������� 73
Cell Scraper������������������������������������������������������������������������������������45
Cell Strainer������������������������������������������������������������������������������������ 75
Cell-Repellent Surface������������������������������������������������44,56,91,92,93
Contact Dishes������������������������������������������������������������������������������173
Containers for Plant Cultures������������������������������������������������������� 192
Cryo Tubes������������������������������������������������������������������������197,199,200
Cryo storage box��������������������������������������������������������������������������� 201
Cryo.s������������������������������������������������������������������������ 197,199,200,201
Cryo.s Biobanking Tubes sterile��������������������������������������������������� 212
Cryo.s Rack Scanner�������������������������������������������������������������������� 214
Cryo.s with Barcode��������������������������������������������������������������203,204
CrystalBridge������������������������������������������������������������������������� 152,153
CrystalQuick����������������������������������������������������������������������������������152
CrystalQuick Plus��������������������������������������������������������������������������152
Datamatrix coded Cryo.s tubes���������������������������������������������203,204
Datamatrix rack for cryo tubes�����������������������������������������������������204
Disposable Inoculation Loops / Needles���������������������������������������175

Disposal Bags��������������������������������������������������������������������������� 47,176
Drosophila Containers������������������������������������������������������������������ 192
EASYstrainer Cell Strainers������������������������������������������������������������ 75
ELISA Microplates������������������������������������������������������������������������� 161
ELISA Strip Plates������������������������������������������������������������������ 163,164
FourWell Plate��������������������������������������������������������������������������������52
Gel-Load Pipette Tips������������������������������������������������������������������� 245
Glass Bottom Microplates������������������������������������������������������������� 119
Grip stopper���������������������������������������������������������������������������������� 182
HTS-Microplates��������������������������������������������� 127,128,129,131,132,135
Immuno Tubes�������������������������������������������������������������������������������166
Inserts for Mini Block Heater��������������������������������������������������������255
Lab Equipment��������������������������������������������������������������� 253,254,255
Leucosep���������������������������������������������������������������������������������������189
Lids������������������������������������������������������������������������������������������� 71,149
Lids / Sealers / CapMats��������������������������������������������������������� 149,150
MASTERBLOCK®����������������������������������������������������������������������137,138
Macro Pipette Tip������������������������������������������������������������������������� 245
MagPen������������������������������������������������������������������������������������������� 97
Magnetic 3D Cell Culture�������������������������������������������������� 95,96,97,98
Magnetic Levitation������������������������������������������������������������������������96
Mass Cell Culture���������������������������������������������������������������������������� 79
MaxiPette������������������������������������������������������������������������������������� 249
Microbiology����������������������������������������������������������������������173,174,175
Microplate Centrifuge������������������������������������������������������������������253
Microplates������������������������������������������������������ 127,128,129,131,132,135
Microplates for Compound Storage���������������������������������������������� 140
Mini Block Heater��������������������������������������������������������������������������255
Mini Centrifuge�����������������������������������������������������������������������������253
Mini Vortex Mixer��������������������������������������������������������������������������254
Molecular Biology����������������������������������������������������� 227,228,229,231
Multi-Channel Pipettes���������������������������������������������������������� 235,236
Multipurpose Containers / Beakers����������������������������������������� 191,192
Multiwell Plates��������������������������������������������������������������� 54,55,56,92
Non-binding Microplates�������������������������������������������������������������� 142
OncoQuick®�����������������������������������������������������������������������������������189
OneWell Plate���������������������������������������������������������������������������������52
PCR 8-Tube Strips�������������������������������������������������������������������������228
PCR Microplates��������������������������������������������������������������������������� 231
PCR Tubes����������������������������������������������������������������������� 227,228,229
Pasteur / Serum Pipettes������������������������������������������������������������� 249
Petri Dishes�����������������������������������������������������������������������������173,174
Pipette Carrousel�������������������������������������������������������������������������236
Pipette Tips�����������������������������������238,239,240,241,242,243,244,245
Pipettes��������������������������������������������������������������������������������� 235,236
Polypropylene Tubes��������������������������������������������������������� 181,182,186
Polystyrene Tubes������������������������������������������������������������� 181,186,187
Polystyrene drosophila containers���������������������������������������������� 192
Products for Microscopy������������������������������������������ 111,113,115,117,119
Protein Crystallisation Plates��������������������������������������������������������152
Rack for pipette tips���������������������������������������������������������������������244
Reaction Tubes���������������������������������������������������������219,220,227,228
Roller Bottles������������������������������������������������������������������������ 78,79,80

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SCREENSTAR Microplates���������������������������������������������������������93,117
Sapphire PCR 8-Cap Strips�����������������������������������������������������������229
Sapphire Pipette Tips��������������������235,238,239,240,241,242,243,244
Sapphire Pipettes������������������������������������������������������������������ 235,236
Screening��������������������������������������������������������������������������������������� 97
Screw Caps���������������������������������������������������������������������������� 212,220
Sealers����������������������������������������������������������������������������������� 149,150
Semi-micro / Macro Cuvette��������������������������������������������������������222
SensoPlate������������������������������������������������������������������������������������ 119
Separation Tubes��������������������������������������������������������������������������189
Serological Pipettes���������������������������������������������������������������247,248
Single-Channel Pipettes�������������������������������������������������������������� 235
Single-break Strip Plates������������������������������������������������������������� 164
Streptavidin-coated microplates������������������������������������������������� 145
Support Rack��������������������������������������������������������������������������������200
Suspension Culture Flasks�������������������������������������������������������������43
Swab Tube�������������������������������������������������������������������������������������175
Terasaki Plates����������������������������������������������������������������������������� 168
ThinCert®���������������������������������������������������������������������62,100,101,102
ThinCert® Cell Culture Inserts����������������������������������������������58,59,60
ThinCert® Plate������������������������������������������������������������������������ 60,102
Transfer Pipettes������������������������������������������������������������������������� 249
Triple Packed Products�������������������������������������������������������41,49,248
Tube������������������������������������������������������������������������������������������������ 73
Tubes / Multipurpose Beakers��������������������������������181,186,187,191,192
Two-position Cap��������������������������������������������������������������������������187
UV-Star® Microplates��������������������������������������������������������������������147
Vortex Mixer����������������������������������������������������������������������������������254
inoculation loop�����������������������������������������������������������������������������175
inoculation needle�������������������������������������������������������������������������175

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