4 100.9). (4ndCo

Flo

4w (11505600,2007300)
1303009100

Flow (11505.9). (4ndCo

2508200600,300)

250

7300

810913010150

10% DBC

150170170

10% DBC

3010% DBC

305050

190
70190

45100
6150Flo)
30000,200w (1CHROMATOGRAPHY

78250
300 9. (4ndCo.9)

470
5100
6150

Flo

7200w (100,250300)

8300 9Nuvia™ S Cation Exchange Media

■■■■

■■

Ultra-High Binding Capacity for Downstream Processes

Chemical stability
to withstandrepetitive cleanin-place cycles

Introduction
Nuvia S is an ultra-high capacity, innovative
cation exchange media built on the industryproven UNOsphere™ base matrix technology.
Nuvia S is a next-generation media thatprovides a unique combination of properties
designed to meet current and future
process needs. Nuvia S delivers superiorbinding capacity over a broad range of pH,conductivity, and flow rates, providing a flexible
process design for both capture and polishingof therapeutic proteins. This exceptional
combination of performance characteristics
makes Nuvia S the tool of choice for a wide
range of downstream processes.

High binding
performance overa wide operating
window
 lexibility in use
Ffor capture orpolish steps
Full support
for regulatory
submission

Nuvia S delivers value and flexibility byallowing users to achieve high binding capacity
at fast flow rates, and by using less media
and smaller columns to purify a given amountof product. Nuvia S can significantly improve
productivity while contributing to reduced
capital costs, buffer consumption,
space requirements, and cycle time fordownstream processes.
Ultra-High Binding Capacity
Nuvia S is designed with proprietary
surface extender technology and tuned for
optimized charge, flow, and binding kinetics.
The unique media design gives Nuvia S
best-in-class dynamic binding characteristics(see Figures 1 and 2).

140
DBC polyclonal hIgG, 10% BT, mg/ml■■

Ultra-high binding
capacity forbiomolecules athigh flow rates

120
100
80
60
40
200Nuvia Smedia

Media1Media
2

Media
3

Media
4

Media
5Media6Fig 1. Binding of polyclonal human IgG (hIgG) by Nuvia S media.
Comparison of Nuvia S and other commercially available CEX
media. Column size, 0.7 x 5.5 cm. The sample was loaded onto
the column in 40 mM NaOAc, pH 5.0 + 30 mM NaCl, washed,
and then eluted with 40 mM NaOAc, pH 5.0 + 1 M NaCl.
BT, breakthrough; DBC, dynamic binding capacity.
2 0200
0DBC mAb, 10% BT, mg/ml

■■

5 150
0G-1 (mg/ml)
5 % B re a k th ro u g h

1 01000

1 0 % B re a k th ro u g h

DBC mAb
5 050

00002246810124681012C o n d Conductivity,u c tiv ity ( mS/cm
m S /c m )1414Fig. 2. Binding of a monoclonal antibody (mAb) byNuvia S media. Column size, 1.1 x 10 cm. The sample
mAb (4.5–5.0 mg/ml) was loaded onto the column in 20 mM
NaOAc, pH 5.0 + NaCl (adjusted for conductivity) until
5% (◆) and 10% (●) breakthrough capacity were observed.
BT, breakthrough; DBC, dynamic binding capacity.

.9). (4ndCoSuperior Flow Properties for Bioprocess
Nuvia S is designed to meet the pressure-flow
requirements of today’s as well as tomorrow’s
demanding purification processes. With optimized beadsize distribution and mechanical properties, Nuvia S
consistently delivers very high binding capacity at fast
linear velocities, while maintaining low column pressure(Figure 3). The wide operating window allows users
to operate at increasingly higher flow rates withoutcompromising binding capacity.
DBC polyclonal hlgG, 10% BT, mg/ml

200

Reproducible Performance
Nuvia S is designed with demanding downstream
processes in mind. Produced in a validated manufacturing
process, the strict specifications of Nuvia S ensurebatch-to-batch consistent performance. A dedicatedapplication team with extensive experience in processdevelopment is available to answer questions and assist
in process separation design.
Properties of Nuvia S MediaType of ion exchanger

Strong cation

Functional group–SO3 –

Total ionic capacity

90–150 µeq/ml

150

Dynamic binding capacity*
300 cm/hr≥110 mg/ml

100

Shipping counterion

Na+Median particle size

85 ± 15 µm

Recommended linear flow rate range**

50–600 cm/hr

Chemical stability
1.0 N NaOH (20°C)
0.1 N NaOH (20°C)***Up to 1 week
Up to 5 years

Gel bed compression ratio		

1.15–1.18 (settled bedvolume/packed bed volume)

pH stability***
Short-termLong-term

2–14
4–13

Shipping solution

20% ethanol + 0.1 M NaClRegeneration

1–2 M NaClSanitation

0.5–1.0 N NaOH

Storage conditions

20% ethanol or 0.1 N NaOH

500150300450600Linear velocity, cm/hrFig. 3. Dynamic binding capacity vs. flow velocity. A 1.1 cm
diameter column was packed to a 20 cm bed height with Nuvia Smedia. 4.5 mg/ml polyclonal human IgG in 40 mM NaOAc and
30 mM NaCl, pH 5.0, was loaded until 10% breakthrough wasobserved. BT, breakthrough; DBC, dynamic binding capacity.

Base-Stable, Consistent Performance
The chemical stability of Nuvia S allows the media
to withstand repeated exposure to commonly usedsanitization and clean-in-place protocols. Nuvia S
exposed to 840 hr of 1.0 M NaOH showed no decreasesof dynamic binding capacity or recovery (Figure 4).
Nuvia S delivers value and process stability bymaintaining peak performance over an extended life.
160160
DBC polyclonal hlgG, 10% BT, mg/ml

140140120120100100h u Ig G B in d in g

Technical Assistance
Regulatory support files are available upon request.
Bio-Rad Laboratories is an ISO 9001 registeredcorporation. For additional information and technicalassistance, contact your local Bio-Rad office.

C a p a c it y , 1 0 % B T

8080

Re co v e r y ( % )

6060
4040
202000*	10% breakthrough capacity determined with 4.5 mg/ml human IgG in
40 mM NaOAc + 30 mM NaCl, pH 5.0.
**	Recommended elution flow rates are <150 cm/hr to minimizeviscosity-induced backpressure.
*** Data derived under accelerated conditions at 60°C.

For more information about Bio-Rad’s complete lineof process chromatography media, please visit us atwww.bio-rad.com/nuvia.

Ordering Information
001

1

2

2

3

3

Duration, weeks

44

55

Duofration S(wee ks)
Fig. 4. Stability
Nuviamedia.
Results from an accelerated
storage study (in 1.0 M NaOH) show no loss in dynamic binding
capacity and recovery. ◆, hIgG binding capacity, 10% breakthrough;■, recovery (%). BT, breakthrough; DBC, dynamic binding capacity.

Catalog#Description

156-0311
156-0313
156-0315
156-0317732-4701
732-4801
732-4802732-4720
732-4740

Nuvia S Media, 25 ml
Nuvia S Media, 100 ml
Nuvia S Media, 500 ml
Nuvia S Media, 10 L
Foresight™ Nuvia™ S Plates, 20 μl*
Foresight Nuvia S RoboColumn Unit, 200 μl**
Foresight Nuvia S RoboColumn Unit, 600 μl**
Foresight Nuvia S Column, 1 ml
Foresight Nuvia S Column, 5 ml

* Package size: 2 x 96-well plates.
** Package size: one row of eight columns.

Bio-RadLaboratories, Inc.
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Taiwan 886 2 2578 7189 Thailand 800 88 22 88 United Kingdom 020 8328 2000

Life ScienceGroup

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