Electrophoresis + Imaging + Analysis

Biologics Analysis Workflow
Integrated Protein Electrophoresis Solutions

Visit bio-rad.com/BiologicsAnalysis for more information.

Biologics Analysis Workflow
Integrated Protein Electrophoresis Solutions

Faster, easier, and more accurate
analysis tools optimized for GMP labs
The Biologics Analysis Workflow is a suite of products designed and validated to assess thepurity of proteins in a GMP regulatory environment. This validated workflow provides increased
electrophoresis throughput, simplified and reproducible staining, and easy data analysis.

1Separate Samples

2 | BIO-RAD LABORATORIES, INC.

2Stain ProteinsSENSITIVITY

THROUGHPUT

3Visualize Proteins

AUTOMATIONREPRODUCIBILITY

4Analyze Results

BIO-RAD LABORATORIES, INC. | 3

1Faster separations using
Criterion TGX Precast Gels

 riterion TGX Precast Gels —C­ increased throughput
with fast run times and an expanded number ofsample loading wells
The unique properties of TGX chemistry allow
Criterion TGX Gels to be run in as little as 20 minutes. TheCriterion format is mid-sized, making it possible to load up to26 samples without decreasing the resolution required foranalysis. In addition to throughput, the Criterion TGX Gels
bring ease to daily lab operations with a one-year shelf
life and utilization of the standard Laemmli buffer system.
Furthermore, the unique design of the Criterion Cell upper
buffer chamber reduces the amount of running buffer
needed, saving on running costs.

More Sample Loading Wells

Fast Run Times
Criterion TGX Gels can be run in as little as 20 min at 300 V
without compromising performance.
Criterion TGX 4–20% Gel
R 1 2 34 5 6 7 8

9 10 11 12 R

Novex 4–20% Tris-Glycine Gel
R 1 2 3 4 5 67 8

9 10 11 12 RCriterion TGX Gels can be used to run up to 26 samples.

Criterion TGX 4–15% Gel
R 1 2 3 45 67 8NuPAGE 4–12% Bis-Tris Gel
9 10 11 12 R

R12 3

4 5 678

9 10 11 12 R

Any kD Criterion TGX Gel
R 1 2 345 6 78

9 10 11 12 R

Comparison of Criterion TGX gels with Thermo Fisher Scientific Novex TrisGlycine Gels and NuPAGE Bis-Tris Gels run at 300 V. All gels were loaded asfollows: outer reference lanes (R), 3 μl Precision Plus Protein Dual Color Standards;
lanes 1 and 12, 10 μl Precision Plus Protein Dual Color Standards; lane 2, 10 μl
Unstained SDS-PAGE Broad Range Standards; lanes 3–5, 15 μl mouse liver extract;lanes 6–8, 15 μl Escherichia coli lysate; lanes 9–11, 15 μl mouse brain extract.
Following electrophoresis the gels were stained with Bio-Safe Coomassie Stain.

4 | BIO-RAD LABORATORIES, INC.

2

Easier staining withQC Colloidal Coomassie Stain

QC Colloidal Coomassie Stain — designed forquantitative analysis of proteins
QC Colloidal Coomassie Stain is extremely sensitive,
capable of detecting nanogram levels of protein. The stain
allows for flexibility in the workflow with the ability to stain
in as little as 1 hour or as long as overnight. Additionally,
the stain allows for very rapid destaining, in as little as
1 hour. The stain is ready to use, with no need for mixing
or addition of other reagents, removing any chance ofvariation due to preparation errors. Furthermore, thestain utilizes ethanol in place of methanol, improving safety
and ease of disposal. The colloidal chemistry enables ahigh protein-to-dye binding ratio, reducing backgroundstaining and improving sensitivity. QC Colloidal Coomassie
Stain is ideal for quantitative analysis, as it is an end-point
stain designed and optimized for use with the GS-900
Calibrated Densitometer.

Criterion TGX 4–15% Gel

NuPAGE 4–12% Gel

Comparison of Bio-Rad Criterion TGX 4–15% Precast
Gel stained with Bio-Rad QC Colloidal Coomassie
Stain (left) and Thermo Fisher NuPAGE 4–12% Precast
Gel stained with Thermo Fisher Colloidal Blue Staining
Kit (right). Both gels were stained overnight followed by a3 hr destain.

Highly Sensitive, Flexible End-Point Stain
The Bio-Rad QC Colloidal Coomassie Stain combines excellent
sensitivity with the flexibility to stain very quickly or for an extended
amount of time while competitor products restrict staining time.
The Criterion TGX Gel stained with QC Colloidal Coomassie Stain
provides increased resolution with sensitivity equivalent to that ofboth the Thermo Fisher SimplyBlue SafeStain and Colloidal Blue
Staining Kit. Additionally, the Bio-Rad QC Colloidal Coomassie
Stain allows for flexibility in staining time, whereas Thermo Fisher
stains are restricted to specific staining times, requiring labs toswitch between stains based on the required stain time of eachof their biologic products.

Criterion TGX 4–15% Gel

NuPAGE 4–12% Gel

Comparison of Bio-Rad TGX 4–15% Precast Gel
stained with Bio-Rad QC Colloidal Coomassie Stain(left) and Thermo Fisher NuPAGE 4–12% Precast Gel
stained with Thermo Fisher SimplyBlue SafeStain(right). Both gels were stained for 1 hr followed by a 1 hrdestain.

BIO-RAD LABORATORIES, INC. | 53More reproducible visualization
using the GS-900 Densitometer

GS-900 Calibrated Densitometer — reproducible
visualization of proteins across a wide dynamic range
The GS-900 Calibrated Densitometer was designed with afocus on accuracy and reproducibility. To ensure the accuracyof each scan, the GS-900 Calibrated Densitometer contains
an internal optical density tablet, which is scanned and used
for calibration before each run. For additional assurance, thecalibration of the GS-900 Densitometer can be validated using
the GS-900 Calibrated Densitometer Installation and Operation
Qualification (IQ/OQ) Kit. This kit utilizes a NIST-traceable external
target to confirm the accuracy of the internal target, guaranteeing
accurate and reproducible results. With red-, green-, and blueCCD technology, the GS-900 Densitometer allows imaging andquantitation of colorimetric blots, film, and gels treated with avariety of stains. Additionally, the wide dynamic range permits
quantitation of proteins from 0–3.4 OD, ensuring the detectionof both highly abundant and rare proteins.

Protein Visualization

Specifications
Light source		

LED

Wavelength		

400–750 nm

Operating modes		Transmissive and reflective

Absorbance range				

0–3.4 OD transmissive0–2.7 OD reflective

Scanning area		

29 x 33 cm

Dimensions		

62.7 x 37.6 x 18.5 cm

Weight			16.1 kg

Qualification (IQ/OQ) Kit

6 | BIO-RAD LABORATORIES, INC.

Pixel density		

700 dpi

Resolution		36.3 µm

4Effortless analysis
with Image Lab Software
U.S. Food and Drug Administration

Image Lab Software — U.S. FDA 21 CFR Part 11
compliant, effortless analysis of samples with intuitive,user-friendly interface

Intuitive Automated Analysis

FPO

Analysis of gels is greatly simplified with Image Lab Software.
Users can automate their analyses, saving time and ensuringanalyses are consistent from run to run. Image Lab Software
does not require previous imaging experience in order toproduce optimal gel and blot images. When flexibility isrequired, users can simply modify analysis settings to match
SOPs and fine-tune other settings such as band detection
and background level. Additionally, with the optional 21 CFRPart 11 module, Image Lab Software contains powerful user
account controls and data security features to help you meet
regulatory requirements.

Easily Customizable Reports

21 CFR Part 11BIO-RAD LABORATORIES, INC. | 7Ordering InformationCriterion TGX Midi Precast Protein Gels*
12+2-Well**
45 µl

Description

18-Well
30 µl26-Well
15 µl

Prep+2-Well
800 µl

IPG+1-Well
11 cm IPG Strip

7.5% Resolving Gel

56710235671024

5671025——

10% Resolving Gel

5671033

5671034

5671035——

12% Resolving Gel

5671043

5671044

5671045——

4–15% Linear Gradient5671083

5671084

5671085

5671082

5671081

4–20% Linear Gradient56710935671094

5671095

5671092

5671091

8–16% Linear Gradient5671103

5671104

5671105

5671102

5671101

Any kD Gel

56711235671124

5671125

56711225671121* Criterion TGX Gels are sold as single gels.
** Reference well accommodates 15 µl of markers/standards.

Catalog #Description

Protein Standards
1610393	Precision Plus Protein All Blue Prestained Protein Standards,
Value Pack, 2.5 ml, 250 applications
1610373
Precision Plus Protein All Blue Standards, 500 µl, 50 applications
1610396 	Precision Plus Protein Unstained Protein Standards, 5 ml,
500 applications
1610363
Precision Plus Protein Unstained Standards, 1 ml, 100 applications
Buffers1610732
1610747
1610737
161071010x Tris/Glycine/SDS Buffer, 1 L
4x Laemmli Sample Buffer, 10 ml
2x Laemmli Sample Buffer, 30 ml
2-Mercaptoethanol, 25 ml

Stain
1610803 	QC Colloidal Coomassie Stain, 1 L, ready-to-use, nonhazardous colloidal Coomassie G-250 stain for protein polyacrylamide gels
Criterion Cell and Systems
1656001
Criterion Cell, vertical midi-format electrophoresis cell, includes buffer tank, lid with power cables, 3 sample loading guides(12+2-well, 18-well, 26-well)1656019
Criterion Cell and PowerPac Basic Power Supply, 100–120/220–240 V, includes 1656001 and 1645050
Instrument
1707991 	GS-900 Calibrated Densitometry System, PC-compatible calibrated densitometer, cables, Image Lab Software, optional FDA 21 CFR Part 11and Installation Qualification/Operational Qualification Module
Regulatory Packages
1707993 	GS-900 Calibrated Densitometer Regulatory Tools Package, pkg of 1, set of protocols for validating calibration of the GS-900 Calibrated
Densitometer, NIST-traceable external step tablet, Image Lab Software, Security Edition with FDA 21 CFR Part 11 Module
17006130	Image Lab Software, Security Edition, 1 license, FDA 21 CFR Part 11 module for Image Lab Software, use with GS-900 Calibrated
Densitometer and all models of Bio-Rad GelDoc and ChemiDoc Imaging Systems
17006171	Image Lab Software, Security Edition, 5 licenses, FDA 21 CFR Part 11 module for Image Lab Software, use with GS-900 Calibrated
Densitometer and all models of Bio-Rad GelDoc and ChemiDoc Imaging Systems
17006172	
Image Lab Software, Security Edition, 10 licenses, FDA 21 CFR Part 11 module for Image Lab Software, use with GS-900 Calibrated
Densitometer and all models of Bio-Rad GelDoc and ChemiDoc Imaging Systems
1707994	GS-900 Calibrated Densitometry IQ/OQ Kit, set of protocols for Installation Qualification/Operational Qualification and NIST-traceable external
step tablet for the GS-900 Calibrated Densitometer

Visit bio-rad.com/BiologicsAnalysis for more information.
BIO-RAD is a trademark of Bio-Rad Laboratories, Inc. Image Lab Touch Software is based in part on the work of the CImg project (http://cimg.eu/). See license for details at
http://www.cecill.info/licences/Licence_CeCILL-C_V1-en.html. Image Lab Touch Software is based in part on libraries from GCC runtime and the Gnu C library. All trademarks
used herein are the property of their respective owner.

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