Western Blotting

Fluorescent Western
Blotting Antibodies
Experience Unmatched Sensitivity and Simpler Multiplexing
StarBright Blue Fluorescent Secondary Antibodies deliver lower limits of detection than traditional methods
and even match high sensitivity chemiluminescence. This is possible because they utilize novel fluorescent
nanoparticles, excited by blue light (430–470 nm), that give a signal that is considerably brighter than conventionaldyes. StarBright Antibodies are available in two colors to provide multiplexing flexibility and are conjugated tohighly cross-absorbed goat anti-mouse and goat anti-rabbit IgG to eliminate cross-reactivity.
StarBright Blue Antibodies

Traditional Detection Methods

StarBright Blue 700(blue-excited fluorescence, 20 sec)

IRDye 800CW(infrared-excited fluorescence, Odyssey 9120 Imaging System)

63 pg

32 pg
StarBright Blue 520(blue-excited fluorescence, 20 sec)

32 pg

Alexa Fluor 488(blue-excited fluorescence, 20 sec)

500 pgSuperSignal West Femto ECL(horseradish peroxidase chemiluminescence, 60 sec)

32 pg

Visit bio-rad.com/FluorescentAbs to learn more about StarBright Antibodies.

hFAB Rhodamine Housekeeping Antibodies
One-step detection of common housekeeping proteins
like actin, tubulin, and GAPDH is possible using hFAB
Housekeeping Antibodies. These fluorescent antibodies
are directly labeled with a rhodamine derivative to makesecondary antibodies unnecessary. hFAB Antibodies aregenerated with human combinatorial antibody library (HuCAL)
technology to provide exceptional specificity and eliminate
cross-reactivity.
Detecting Posttranslationally Modified Targets
Fluorescent multiplexing with StarBright Antibodies lets you
detect targets with posttranslational modifications. Their
flexibility and high sensitivity let you visualize unmodified andmodified targets simultaneously — even if only a fraction ofthe protein is modified.
Easy Multiplexing and Versatile Normalization
StarBright Antibodies are easily adapted into your current
protocols. They are the ideal multiplexing partners totraditional secondary antibodies conjugated to DyLightor Alexa Fluor fluorophores. Confidently use them to normalize
your data with either Bio-Rad hFAB Rhodamine
Housekeeping Antibodies or a total protein approach
using Stain-Free technology.

FcFabMonovalent FabComparison of typical IgG and an hFAB Rhodamine Antibody.

Red: Phospho-ERK1/2, StarBright Blue 700
Green: ERK1/2, DyLight 800
Blue: GAPDH, hFAB Rhodamine Antibody

Example of detecting posttranslationally modified targets.

Red: ATG7, StarBright Blue 700
Yellow: Tubulin, hFAB Rhodamine Antibody
Green: AKR1C2, StarBright Blue 520
Blue: TP1 (biotinylated), DyLight 800
Example of quadruplex western blotting targets.

Ordering information
Catalog #Description

StarBright Blue 700 Secondary Antibodies
12004158
Goat Anti-Mouse IgG StarBright Blue 700, 400 µl
12004159
Goat Anti-Mouse IgG StarBright Blue 700, 80 µl
12004161
Goat Anti-Rabbit IgG StarBright Blue 700, 400 µl
12004162
Goat Anti-Rabbit IgG StarBright Blue 700, 80 µl
StarBright Blue 520 Secondary Antibodies
12005866
Goat Anti-Mouse IgG StarBright Blue 520, 400 μl
12005867
Goat Anti-Mouse IgG StarBright Blue 520, 80 μl
12005869
Goat Anti-Rabbit IgG StarBright Blue 520, 400 μl
12005870
Goat Anti-Rabbit IgG StarBright Blue 520, 80 μl

Catalog #Description

hFAB Rhodamine Antibodies
12004163
Anti-Actin hFAB Rhodamine Antibody, 200 µl
12004164
Anti-Actin hFAB Rhodamine Antibody, 40 µl
12004165
Anti-Tubulin hFAB Rhodamine Antibody, 200 µl
12004166
Anti-Tubulin hFAB Rhodamine Antibody, 40 µl
12004167
Anti-GAPDH hFAB Rhodamine Antibody, 200 µl
12004168
Anti-GAPDH hFAB Rhodamine Antibody, 40 µl
Blocking Buffer
12010020
EveryBlot Blocking Buffer, 500 ml

Visit bio-rad.com/FluorescentAbs for more information about StarBright Antibodies.
BIO-RAD is a trademark of Bio-Rad Laboratories, Inc. HuCAL is a trademark of MorphoSys AG in certain jursidictions.
All trademarks used herein are the property of their respective owner.

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