Food and Environmental Testing

RAPID'Chromogenic Media
Culture Media for the Detection and Enumeration ofFood and Environmental Pathogens and Quality Indicators

Visit bio-rad.com/RapidMedia for more information.

RAPID'Chromogenic Media Technology and Applications
RAPID'Chromogenic Media are a complete range of highly selective chromogenic agars designed to detect and enumeratevarious microorganisms in food and environmental samples. They offer economical, labor-saving protocols and provide
a shorter time to results than classical methods. The combination of the selected chromogenic mixture with high-quality raw
material allows for high levels of contrast, selectivity, and sensitivity — making these plates easy to read.

AL (Agar Listeria according to Ottaviani and Agosti)
Applications
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Protocols for detection and enumeration
Approved for ISO 11290-1 and 11290-2
protocols
Recommended in FDA BAM Chapter 10
Approved for MFLP-74 protocol
Confirmation of iQ-Check™ Listeria
monocytogenes and Listeria spp. II
real-time PCR results

Enzymatic Activity

L. monocytogenes

Listeria spp.

Non-Listeria

PI-PLC detection+––b-D-glucosidase++–

RAPID'B.cereus
Applications■■■■

Enumeration of the Bacillus cereus group
Clear enumeration of the sensitive
B. cytotoxicus
No confirmation required
Inhibition of background flora onraw matrices

B. cereus

B. cereus

AtypicalB. cereus(e.g., B. cereus
sensu stricto)

B. cytotoxicus(e.g., B. mycoïdes)(e.g., B. thuringiensis)

Chromogenic substrate++++Phospholipase+++–Enzymatic Activity

RAPID'Campylobacter
Applications
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Protocols for detection and enumeration
Approved as second media inISO 10272-1 protocol
Inhibition of competing organismsValidation with Hunt broth(see USDA MLG)

Enzymatic Activity

Thermophilic Campylobacter spp.

Chromogenic substrate+

RAPID'E.coli 2Applications
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Enumeration of Escherichia coli andother coliforms
Differentiation by color, not gas formation
Can be incorporated into a hygienemonitoring program
No confirmation required

Enzymatic Activity

E. coli

Other Coliforms

Non-Coliforms

Glucuronidase+––Galactosidase++–E. coli O157:H7

Other E. coli

Other Bacteria

RAPID'E.coli O157:H7Applications
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Detection of E. coli O157:H7
Approved as second media inISO 16654 protocol
Enzymatic Activity
Confirmation of iQ-Check E. coli 		O157:H7 real-time PCR results
Glucuronidase
Detection of rare sorbitol-positive 		
Galactosidasestrains
Sorbitol

2 | BIO-RAD LABORATORIES, INC.

–+–++––++/–RAPID'Enterobacteriaceae
Applications
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Enumeration of Enterobacteriaceae
Surface inoculation or pour-plate method
Can be incorporated into a hygienemonitoring program
No confirmation required

Enzymatic Activity

Enterobacteriaceae

NonEnterobacteriaceae

Glucose fermentation+–RAPID'Listeria spp.
Applications■■■

Detection of Listeria spp.
Confirmation of iQ-Check Listeria spp.
real-time PCR results
Can be part of an environmental
monitoring program

Enzymatic Activity

Listeria spp.

+b-D-glucosidase

RAPID'L.monoApplications
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Enumeration and detection ofL. monocytogenes and detection ofListeria spp.
Approved as second media inISO 11290-1 protocol
Recommended in FDA BAM Chapter 10
Approved for MFHPB-30 andMFLP-74 protocols
Confirmation of iQ-Check Listeria
monocytogenes II real-time PCR results

Enzymatic ActivityL.
monocytogenes

L.
ivanoviiL.
innocuaL.
welshimeri

PI-PLC detection++––

Xylose fermentation–+–+

Enzymatic Activity

Cronobacter spp.

OtherEnterobacteriaceae

a-D-glucosidase+–RAPID'Sakazakii
Applications■■■

Detection of Cronobacter spp.
Confirmation of iQ-Check Cronobacter
spp. real-time PCR results
Harmonized enrichment protocol with
RAPID'Salmonella for select matrices

RAPID'Salmonella
Applications
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Detection of Salmonella spp.
Single 24 hr enrichment protocol without
subculturing
Approved as second media inISO 6579-1 protocol
Confirmation of iQ-Check Salmonella II
real-time PCR results
Harmonized enrichment protocol with
RAPID'Salmonella for select matrices

Salmonella
spp.

OtherEnterobacteriaceaeProteus
spp.

C8 esterase++–b-D-glucosidase–+–

Enzymatic Activity

RAPID'StaphApplications■■■

Enumeration of coagulase-positive
staphylococci
Final result in 24 hr
Fast latex confirmation

Enzymatic Activity

Staphylococcus
Coagulase-Positive

Staphylococcus
Coagulase-Negative

Potassium tellurite
reduction++

Egg yolk proteolysis+–BIO-RAD LABORATORIES, INC. | 3Validations
NF Validation
by AFNOR
Certification

Chromogenic MediaRESEARCH
INSTITUTEAOACAL•RAPID'B.cereus•

RAPID'Campylobacter••

RAPID'E.coli 2••

RAPID'E.coli O157:H7••

RAPID'Enterobacteriaceae•

NordVal•HealthCanada•

RESEARCH
INSTITUTE

Chromogenic MediaNF Validation
by AFNOR
Certification

AOAC

RAPID'Listeria spp.

••

RAPID'L.mono•

RAPID'Sakazakii•

RAPID'Salmonella•

NordValHealthCanada

•

•••

•
•

RAPID'Staph

Ordering Information
Catalog #Description

Buffered Peptone Water Plus Broth3554179
Ready to use, 225 ml x 6 bottles
3555795
Ready to use, 3 L x 4 bags
3555790
Ready to use, 5 L x 2 bags
3564684
Dehydrated base, 500 g
Buffered Peptone Water Standard Broth
12013260
Ready to use, 5 L x 2 bags
12013259
Dehydrated base, 500 g
12013258
Dehydrated base, 5 kg
Fraser ½ Plus Broth3564604
Dehydrated base, 500 g
3564616
Supplement, 1 box, 10 vials
3555797
Ready to use, 225 ml x 6 bottles
3555794
Ready to use, 3 L x 4 bags
Half Fraser Standard Broth12018707
Ready to use, 5 L x 2 bags
12018706
Dehydrated base, 500 g
12018705
Dehydrated base, 5 kg
AL35639653563695
35640433555200
35640413564042

Ready to use, 90 mm x 120 plates
Ready to use, 90 mm x 20 plates
Dehydrated base, 500 g
Ready to use, 6 x quantity for 250 ml
Supplement 1, 1 box, 10 vials
Supplement 2, 1 box, 10 vials

RAPID'B.cereus Agar
12007304 	Ready to use, 90 mm x 20 plates
12007305
Dehydrated base, 500 g
12007306
Supplement 1, 1 box, 10 vials
12007307
Supplement 2, 1 box, 10 vialsCatalog #Description

RAPID'Campylobacter Agar
3564295
Dehydrated base, 500 g
3564296
Supplement, 1 box, 10 vials
3564297 	Campylobacter Confirm Latex Kit,
50 tests (confirmation)
12012036
Ready to use, 90 mm x 20 plates
RAPID'E.coli 2 Agar
3555299
Ready to use, 100 ml x 6 bottles
3555297
Ready to use, 200 ml x 6 bottles
3564024
Dehydrated base, 500 g
RAPID'E.coli O157:H7 Agar
3564748
Dehydrated base, 100 g
RAPID'Enterobacteriaceae Agar
3554012
Ready to use, 200 ml x 6 bottles
3564004
Dehydrated base, 500 g
RAPID'Listeria spp. Agar
3564744
Dehydrated base, 500 g
3564745
Supplement 1, 1 box, 10 vials
3564746 		
Supplement 2, 1 box, 10 vials
RAPID'L.mono Agar
3563964
Ready to use, 90 mm x 120 plates
3563694
Ready to use, 90 mm x 20 plates
3555294
Ready to use, 190 ml + supplements
3564293
Dehydrated base, 500 g
3564294
Supplement 1, 1 box, 10 vials
3564746
Supplement 2, 1 box, 10 vials
3553669
Rhamnose Test, 1 ml x 28 tubes

Catalog #Description

RAPID’Sakazakii Agar
3563971
Ready to use, 90 mm x 20 plates
3564976
Dehydrated base, 500 g
12013322
PIF Supplement, 2 g
RAPID'Salmonella Agar
3563961
Ready to use, 90 mm x 20 plates
3563963
Ready to use, 90 mm x 120 plates
3564705
Dehydrated base, 500 g
3564709 	Enrichment supplement capsules,
10x, 100 capsules
3564710 	Enrichment supplement capsules,
100 capsules
3564712 	Enrichment supplement, 100analyses
3556710 	Salmonella Latex Test, 75 tests(confirmation)
3556711 	Salmonella Confirm Latex Kit,
50 tests (confirmation)
12013322
PIF Supplement, 2 g
RAPID'Staph Agar
3563960
Ready to use, 90 mm x 20 plates
3564704
Dehydrated base, 500 g3556356	Pastorex Staph Plus Latex
Agglutination Test, 50 tests
3556353	
Pastorex Staph Plus Latex
Agglutination Test, 5 x 50 tests

For more information and to see how our products can be incorporated into food testing workflows, visit our website:
RAPID'Chromogenic Media — bio-rad.com/RapidMedia
iQ-Check Real-Time PCR Kits — bio-rad.com/iQCheck
BIO-RAD is a trademark of Bio-Rad Laboratories, Inc. IQ-CHECK is a trademark of Bio-Rad Europe GmbH in certain jurisdictions. All trademarks used herein are the propertyof their respective owner. © 2024 Bio-Rad Laboratories, Inc.

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