Comparison of DNA from blood, Oragene®/saliva and buccal
swab samples on the Illumina® BeadChip 610Quad assay
Rafal Iwasiow†, Scott Rabuka†, Susumu Saito‡, Fumiko Komine‡, Hiroko Sato‡, Kazuna Ichikawa‡,
Takaaki Kuramoto‡ and Mikio Kawahara‡† DNA Genotek, Inc., Ottawa, Ontario, Canada‡ RIKEN GENESIS Co., Ltd., Riken Yokohama Institute, Tsurumi-ku, Yokohama-shi, Kanagawa, Japan

Introduction
Non-invasive and easy-to-use DNA sample collection technologies
can greatly improve the success of a research study by ensuring donor
compliance and acceptance into a study. However, not all non-invasive
collection kits offer the same analytical performance. The purpose of thisstudy is to demonstrate the performance benefits of DNA from Oragene®/saliva samples over DNA collected from buccal swab samples. Similarcollection benefits can be appreciated with both of these methods;
however their respective performance is far from similar. Oragene[DNA Genotek] is a non-invasive sample collection method that yields
large amounts of high quality DNA from a 2 mL saliva sample that isstable for several years at room temperature.

Both blood and saliva had sufficient DNA with A260/A280 ratios having
averages greater than 1.7. In contrast, the buccal collection had much
lower amounts of DNA and poor A260/A280SBUJPT "WFSBHF <'JHVSF>
It should be noted that after sample normalization the amount of DNA as
quantified by PicoGreen was not the expected 100 ng/µL: for blood theaverage was 80 ng/µL and for saliva the average was 30 ng/µL. The lower
than expected values, as measured by PicoGreen, are due to quantificationof RNA fragments by absorbance. Considering that saliva containsconsiderably more RNA than blood this resulted in lower DNA quantification
for saliva. This illustrates the importance of properly quantifying samples
by PicoGreen.

DNA from blood, Oragene/saliva and buccal swab samples were collected,
purified and processed on the Illumina® BeadChip 610Quad assay.
DNA from blood is used as the control sample in this study.

"260"280SBUJPTSamples from 16 donors were collected using 3 different methodologies.
A – Venous blood collection using EDTA tubes, B – Saliva collection using
0SBHFOFt%/" 0( GSPN%/"(FOPUFL $oCVDDBMTBNQMFVTJOHB
cotton swab.
DNA from each sample was purified and processed on the Illumina
BeadChip 610Quad. After purification, each sample was quantified using
absorbance and the A260/A280 ratios were determined on a Nanodrop
instrument [see Figure 1]. The samples were then normalized to 100 ng/µL
and re-quantified using PicoGreen® [see Figure 2]. The diluted samples
were then processed on the Illumina BeadChip 610Quad as per
manufacturer’s instructions.
2VBOUJöDBUJPOPGTBNQMFTCZBCTPSCBODF "260)550
500
450400ng/µL

350

Blood

Oragene/saliva

BuccalFigure 3"260"280SBUJPTGPSCMPPE TBMJWBBOECVDDBMTBNQMFTConclusion
When all samples were processed on the Illumina BeadChip 610Quad both
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*MMVNJOB2VBE#FBE$IJQDBMMSBUFT

300
250
200
150100500Blood

Oragene/saliva

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TBNQMFT͇BT͇NFBTVSFE͇CZ͇BCTPSCBODF "260).

1JDP(SFFORVBOUJöDBUJPOPGOPSNBMJ[FETBNQMFT
90

1.02
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0.99
0.98
0.97
0.96
0.95
0.94
0.93
0.92
0.91
0.90
0.89
Blood

80

Oragene/saliva

BuccalFigure 4*MMVNJOB2VBE#FBE$IJQDBMMSBUFTGPSCMPPE TBMJWBBOECVDDBMTBNQMFT#MPPE͇BOE͇TBMJWBIBEFYDFMMFOUDBMMSBUFT#VDDBMTBNQMFTIBETJHOJöDBOUMZMPXFS
DBMM͇SBUFT͇EVFUPMPXFS%/"RVBMJUZ "260"280 BOEMPXFS%/"RVBOUJUZ "260).

7060
ng/µL

A260/A280

Methods and results

2.1
2.0
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1.8
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1.2
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50

Oragene is a proven non-invasive collection method that can bereliably used for microarray testing as demonstrated in this study.

403020100Blood

Oragene/saliva

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641BUFOU/P  &VSPQFBO̓1BUFOU/PBOE̓1BUFOUQFOEJOH
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Superior samples t1SPWFOQFSGPSNBODF

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