Chromatography Resins

Monoclonal Antibody Purification Resin Selection Card
Bio-Rad offers a wide range of resins for each phase of monoclonal antibody process purification, at scales ranging
from nanograms to kilograms.
Use this card to select the optimal resin for your monoclonal antibody process purification.

Visit bio-rad.com/web/mAbChromResins for more information.

Optimal Resin for Monoclonal Antibody Purification
Purification Step
Property

CaptureCapture andIntermediate

Resin type

UNOsphere SUPrA™

Nuvia™ S

UNOsphere™ Q

Nuvia Q

Nuvia HR-S

CHT™ Ceramic
HydroxyapatiteNuvia™ cPrime™

Chromatography Affinity
type

Cation exchange

Anion exchange

Anion exchange

Cation exchange

Mixed-mode(cation exchange
and metal affinity)

Mixed-mode (HICand cation exchange)

Particle size

53–61 µm

85 ± 15 µm

120 µm85 ± 15 µm

50 ± 10 µm

20 ± 2, 40 ± 4, 80 ± 8 µm 70 ± 10 µm

Ionic capacity

N/A

90–150 µeq/ml

120 µeq/ml

100–170 µeq/ml

100–180 µeq/ml—110–150 µeq/ml≥110 mg/ml
at 300 cm/hr*≥180 mg BSA/ml
at 150 cm/hr*≥170 mg/ml
at 300 cm/hr*≥70 mg/ml
at 300 cm/hr*≥25 mg lysozyme/g*>40 mg hIgG/ml(at 10% breakthrough)at 300 cm/hr*

Dynamic binding 30 ± 3 mg/ml*
capacity (DBC)

Intermediate

Intermediate and Polish25–60 mg IgG/ml
at 300 cm/hr*≥125 mg BSA/ml
at 600 cm/hr*>60 mg lactoferrin/ml*

Recommended
linear flow rate

100–600 cm/hr

50–300 cm/hr

50–300 cm/hr

50–600 cm/hr

50–200 cm/hr

50–300 cm/hr

50–600 cm/hr

pH stability

3–11

Short-term: 2–14

1–14

Short-term: 2–14

Short-term: 2–14

6.5–14

Short-term: 3–14

Long-term: 4–12

Long-term: 4–13Long-term: 4–13

Long-term: 4–13

BSA, bovine serum albumin; HIC, hydrophobic interaction chromatography.
Refer to bulletin 6875 to see how these resins help in monoclonal antibody process purification.
* Go to the product detail page or bulletin 6713 on bio-rad.com to see how the DBC was determined and for other technical details.

Ordering Information
CaptureCapture and Intermediate

Intermediate

UNOsphere SUPrA

Catalog #Size

Nuvia S

Catalog #Size

UNOsphere Q

Catalog #SizeForesight™ UNOsphere
SUPrA RoboColumn Unit732-4834

200 µlForesight Nuvia S Plates

732-4701

600 µlForesight UNOsphere QPlates

732-4714

732-4835

2 x 96-well,
20 µl

2 x 96-well,
20 µl

Foresight UNOsphere
SUPrA Column732-4729

1 ml

200 µl200 µl732-4802

600 µlForesight UNOsphere Q
RoboColumn Unit

732-4819

5 ml

Foresight Nuvia S
RoboColumn Unit

732-4801

732-4749

732-4820

600 µlUNOsphere SUPrA Affinity
Chromatography Resin

1560218

25 ml

Foresight Nuvia S Column732-4720

1 x 1 ml

732-4732

1 ml

1560219

100 ml732-4740

1 x 5 mlForesight UNOsphere Q
Column732-4752

5 ml

156-0220500 ml156031125 ml

UNOsphere Q Resin

1560101

25 ml

156-0221

5L

1560313

100 ml1560103

100 ml

10 L

156-0315

500 ml

156-0105

500 ml

156-0317

10 L

156-0107

10 L

156-0222

Nuvia S Resin

Intermediate and PolishIntermediate and PolishNuvia Q

Catalog #Size

Nuvia HR-S

Catalog #SizeForesight Nuvia Q Plates
Foresight Nuvia Q RoboColumn Unit

732-4703
732-4804
732-4805
732-4721
732-4741
15604111560413156-0415
156-0417

2 x 96-well, 20 µl
200 µl600 µl1 x 1 ml1 x 5 ml
25 ml
100 ml500 ml10 L

Foresight Nuvia HR-S Plates
Foresight Nuvia HR-S RoboColumn Unit

732-4707
732-4831
732-4832
732-4723
732-4743
15605111560513156-0515
156-0517

2 x 96-well, 20 µl
200 µl600 µl1 x 1 ml1 x 5 ml
25 ml
100 ml500 ml10 L

CHT Ceramic Hydroxyapatite

Catalog #Size

Nuvia cPrime

Catalog #SizeForesight CHT Type I Plates

732-4716

Foresight Nuvia cPrime Plates
Foresight Nuvia cPrime RoboColumn Unit

Foresight CHT Type II Plates

732-4718

Foresight CHT Type I RoboColumn Unit732-4822
732-4823
732-4825732-4826732-4735
732-4755
732-4736
732-4756
1584000
1570040
157-0041
157-0045
1588000
1570080
157-0081
157-0085
1584200
1574000
157-4100
157-4500
1588200
1578000
157-8100
157-8500

40 µm, 2 x 96-well,
20 µl
40 µm, 2 x 96-well,
20 µl
40 µm, 200 µl
40 µm, 600 µl
40 µm, 200 µl
40 µm, 600 µl
40 µm, 1 ml
40 µm, 5 ml
40 µm, 1 ml
40 µm, 5 ml
10 g
100 g
1 kg
5 kg
10 g
100 g
1 kg
5 kg
10 g
100 g
1 kg
5 kg
10 g
100 g
1 kg
5 kg

732-4705
732-4807
732-4808
732-4722
732-4742
1563401
1563402156-3403
156-3404
156-3405
156-3406

2 x 96-well, 20 µl
200 µl600 µl
1 ml
5 ml
25 ml
100 ml500 ml
1L5L
10 L

Foresight Nuvia Q Column
Nuvia Q Resin

Intermediate and Polish

Foresight CHT Type II RoboColumn Unit
Foresight CHT Type I Column
Foresight CHT Type II Column
CHT Type I Media, 40 µm

CHT Type I Media, 80 µm

CHT Type II Media, 40 µm

CHT Type II Media, 80 µm

Foresight Nuvia HR-S Column
Nuvia HR-S Resin

Intermediate and Polish

Foresight Nuvia cPrime Column
Nuvia cPrime Resin

RoboColumn is a trademark of Atoll GmbH.

Visit bio-rad.com/web/mAbChromResins for more information.

Bulletin 6786 Ver A

16-04190516