EXPLORE

OUR WORLD OF

MICROPLATESMicroplate Selection Guide

GREINERWORLD OF M

CELL CULTURE(p. 8)
Adherent

Non-adherent/suspension

CELLSTAR® TC

CELLSTAR®
suspension

CELLCOAT®
protein coating

CELLSTAR®
cell-repellent

Advanced TC

STORA(p. 16

MICROSCOPY(p. 18)Polyprop& cycloo
plateµClear®
film bottom

2/

www.gbo.com

Cycloolefin
film bottom

Glass bottom

BIO-ONEMICROPLATESSCREENING(p. 12)

Non-treatedHigh binding /sterileNon-binding

UV
spectroscopy

Covalent
binding

Streptavidin
coating

AGE

6)

IMMUNOLOGY(p. 14)pyleneolefines

Med. binding

High-binding

www.gbo.com

/3

PS, PPOR COC?
What is the rightbase material foryour application?

4/

www.gbo.com

1. INTRODUCTION
Continued progress in researchand related technologies,
such as microscopy, imaging,detection and liquid handlingsystems, has given rise to awide variety of platforms usedin basic science, biotechnology
andpharmaceuticaldrugdevelopment.
Today,
researchers need to selectapplication-specificmicroplates among a broad range of
products that differ in format,
design, base material, colour,
surface properties and bottom
configuration. The intent of
this brochure is to provide
an overview of microplates
available from Greiner Bio-One,
with a focus on applications.

2. GENERAL MICROPLATE FEATURES
2.1 BASE MATERIAL
Polystyrene is the most extensively used material for plastic
laboratory ware. The highly
transparent resin is ideallysuited for both microscopic
imaging and optical measurements. Due to its chemical
nature, polystyrene is a hydrophobic compound; however, its
properties can be adjusted witha variety of physical surface
treatments or coatings to accommodate requirements for
multiple diverse applications.

Polypropylene is characterised by a high resistance tocommon chemicals (e.g. DMSO)
and thermal stability (-196 °C to+121 °C). Polar molecules likeDNA or proteins are binding
less to polypropylene than topolystyrene. One drawbackof polypropylene is its limited
transparency; however, thisfeature is not typically required
for the primary application ofstorage plates and vessels.
Commonly, vessels made ofpolypropylene are not surface
treated or coated.

CHEMICALSTRUCTURE
of microplatebase materials
Polystyrene (PS)Polypropylene (PP)www.gbo.com

/5

Cycloolefin microplates
are suited for spectroscopic
measurements andsophisticated opticalrequirements.

Cycloolefin is the material of
choice for microplates withspecial requirement profiles.
A low level of autofluorescence, along with exceptional transparency in lower UV
wavelengths, enables cycloolefin microplates to be
utilised for spectroscopic
measurements in the UV range(UV-Star ® microplates). The
chemical stability of cycloolefin to polar solvents like DMSO,
together with an extraordinarily low vapour diffusionrate, render the base materialvery suitable for the production of compound storage microplates, and the dimensional
stability is additionally beneficial for microplate use within
fully
automatedsystems.
Moreover, cycloolefin’s glasslike optical properties, whencombined with a respective
surface treatment, facilitate
use of cycloolefin microplates
for cell culture applications
with sophisticated opticalrequirements such as highresolution confocal microscopy and high-content screening.

CHEMICALSTRUCTURE
of cycloolefin
R1

R2

Norbonene (monomer of cycloolefin)

6/

www.gbo.com

Black, whiteor clear?
Find the rightcolour for yourassay!

properties. Polypropylene microplates are as well available
with black and white pigmentation and offer lower biomolecule binding and higher thermaland chemical resistance thanpolystyrene.

2.2 PIGMENTATIONBlack pigmented microplates
are commonly used for fluorescence applications, whereas
white pigmented microplates
typically support luminescencemeasurements, and are sometimes used to enhance fluorescence signal intensity. Bothpigmentations help overcome
critical issues for these techniques, such as background,
autofluorescence, and well-towell crosstalk.

2.3 SURFACE PROPERTIESAt the well surface, interaction
between the sample and themicroplate takes place. Therefore surface properties play animportant role for the functionality of a vessel. Surface properties can be modified in many
ways, whether by physical,
chemical or coating methods,
to fulfill various demands.

Pigmentation does not impact
the material or surface chemistry, and black or white polystyrene microplates are available with different surface

MICROPLATE COLOUR & CORRESPONDING APPLICATIONSColorimetric Measurements

Fluorescence Measurements

Luminescence Measurements

Transparent polystyrene
microplates with solid bottom//

Top reading: 		White microplateswith solid bottom/

Bottom reading& microscopy:
White microplates withtransparent film bottom/

Top reading: 		Black microplates withsolid bottom, white
microplates to enhancesignal intensity
Bottom reading& microscopy:
Black microplates withtransparent film bottom
or glass bottomwww.gbo.com

/7

3. MICROPLATES BY APPLICATION
3.1 CELL CULTURE
3.1.1 Adherent Cell Culture
CELLSTAR ® TC (TC = Tissue
Culture) is the standard surface
for classical cultivation ofadherent cells. CELLSTAR ® TC
products undergo a special
physical surface treatment,
leading to the incorporation ofpolar groups such as carboxyl
and hydroxyl residues, whichfunctionalises the hydrophobicpolystyrene surface to resultin improved, consistent cellattachment. CELLSTAR ® TC
products are sterile, and can be
stored at room temperature.
For fastidious, primary or sensitive cells, cells cultivatedunder restricted growth conditions (serum-free or serum-reduced), or cells stressed bytransduction or transfection,

Greiner Bio-One offers thesynthetic Advanced TC surface
and the CELLCOAT ® productline.
The surface of the Advanced TC
cell culture vessels is chemically modified to positively
influence cellular features andfunctions. Enhanced cell attachment and higher proliferation rates improve and accelerate cell expansion. The positiveeffect of the Advanced TC
surface is particularly apparent following cellular stressinduced by transfection ortransduction processes. In
contrast to biological coatings,
the surface chemistry is synthetic. Advanced TC products
are sterile, and can be stored
at room temperature.

Adipogenesis of humanmesenchymal stem cells on
384 well polystyrene film bottom
microplates (Item No. 781091).

8/

www.gbo.com

CELL CULTURE

The CELLCOAT® product line
comprises cell culture vessels
which are coated with proteins of
the extracellular matrix (CollagenType I, Fibronectin, Laminin)or synthetic proteins (PolyD-Lysine, Poly-L-Lysine). As asynthetic molecule, Poly-Lysine
is free from contamination
with other proteins. Biologicalcoatings facilitate the growthof many cell types, including
hepatocytes, muscle cells,
epithelial/endothelial cells,
neural cells and transfected
cell lines. Many otherwise
difficult-to-cultivate
cells
adhere to biological coatings,

thereby enabling successful
culture. Additionally, for certain
cell lines, protein coating canhave a positive influence on
differentiation and morphology.
CELLCOAT® surfaces are also
highly suitable for serumfree and serum-reduced cell
cultivation, promotion ofcell adhesion and stressfulprocedures like transfection or
automated washing.
For microplates especiallydeveloped to meet therequirements of high-content
screening applications, pleaserefer to chapter 3.5 (p. 18-19).

MICROPLATES FOR ADHERENT CELL CULTURE

PROMOTION OF CELL ATTACHMENTCELLSTAR® TC

CELLCOAT®

Advanced TC/

Standard cell culture

/

/

/

Adherent cell lines

Improved cell adhesion
and proliferation

Cultivation of fastidiousand sensitive cell lines/

Reduced-serum orserum-free cultivation/

Reduced-serum orserum-free cultivation/

Improved growth ofprimary cells/

Improved transfection
and transduction/

Differentiation ofindividual cell types

www.gbo.com

/9

3.1.2 Non-adherent and3D Cell Culture
CELLSTAR ® suspension culture
vessels are well suited for suspension culture of non-adherent cells due to no polarity orasymmetric charge allocationof the surface which does notenable hydrophilic interactions
between the surface and thecells. CELLSTAR® suspension
products feature a stronglyhydrophobic surface and aresterile.
The CELLSTAR® cell-repellent
microplates feature a sterile,innovative chemical surface
modification, which has been
specifically developed to effectively prevent the attachment of semi-adherent andadherent cell lines.
Beside conventional two-dimensional (2D) monolayer cell
culture, 3D cell culture models
are becoming a routine tool toenable the expression of extracellular matrix (ECM) components as well as the formationof cell-cell and cell-matrix interactions. These characteristics are important for replicating in-vivo cell differentiation,
proliferation, and function
in vitro. Inhibition of cell attachment and cell-surface
interaction enables the cellcell interaction to dominate
and make the cell-repellent
surface an ideal platform for
3D cell culture applications
such as the spontaneous formation of spheroids, the cultivation of stem cell aggregates
and as indispensable part ofthe magnetic 3D cell culture
technology. Additionally thesurface does not degrade orleach under common cell
culture conditions, rendering
an ideal substrate for nativecell
cultureexperiments
and long-term cultivation inhydrogels.

96 Well Bioprinting Kit formagnetic 3D cell culture

10 /www.gbo.com

CELL CULTURE

The core technology of Greiner
Bio-One’s Magnetic 3D Cell
Culture (m3D) is the magnetization of cells with biocompatible NanoShuttle™-PL. The
formation of one spheroid per
well in an F-bottom plate withcell-repellent surface is forcedby magnets either by levitationor bioprinting. The magnetic
forces work as an invisible
scaffold that rapidly and gently
aggregates cells and inducescell-cell interactions and ECM
synthesis to form structurally
and biologically representative
3D models in vitro.

The m3D system overcomesthe limitations of other platforms by enabling rapid formation of spheroids, reproducible and scalable in sizefor high-throughput formats(96, 384 and 1536 well) andwithout limitation to cell types.
Together with commerciallyavailable biochemical assays
the m3D technology provides
an ideal combination for
high-throughput
compoundscreening that encompasses
pre-animal toxicity screens,anti-cancer agents, cardiovascular drugs and well as manyother important drug discovery
targets.

MICROPLATES FOR NON-ADHERENT CELL CULTURE

PREVENTION OF CELL ATTACHMENTCELLSTAR® suspension

CELLSTAR® cell-repellent/

Non-adherent cells/

Spheroid culture of tumour cells/

Hybridoma and embryonic stem cells/

Aggregation of stem cells/

Suspension-adapted cells/

Suspension culture of semi-adherent
and adherent cell lines/

3D culture in hydrogels

www.gbo.com

/ 11

3.2 SCREENING AND UV/VIS SPECTROSCOPY
For biochemical screening applications, microplates madeof polystyrene without surface
treatment (non-treated) arefrequently the plate of choice.
Greiner Bio-One polystyrene
microplates are manufactured of carefully selected raw
material batches and demonstrate reproducibly low biomolecular binding. Due to theirmaterial properties, polypropylene microplates (see also
chapter 3.4, p. 16-17) featureless biomolecule adsorption
than polystyrene. However,for very sensitive applications,even low amounts of biomolecular binding can interferewith the assay.
Greiner Bio-One’s non-binding surface for microplates
effectively prevents binding.
Characterised by low protein,
peptide, DNA and RNA binding
properties, the non-binding surface increases assay
sensitivity by reducing background and, therefore, improving signal-to-noise ratio.
The non-binding surface is

SCREENING MICROPLATESPROTEIN ADSORPTION

12 /Non-binding
PS microplates

Polypropylene
microplates

Untreated
PS microplatesHigh-binding
PS microplates//

Storage applications

/

/

/

Biochemical assays

Homogeneous
assaysHeterogeneous
assays/Biochemical assays/

Immunological assays(ELISA, FIA, LIA)  p. 14

Sensitive
biochemical assays

www.gbo.com

SCREENING

achieved through a chemical
modification of the microplatesurface. It remains stable
under common assay conditions, and does not degrade
during short-term storage.
High-binding polystyrene microplates can be used for
applications where sterile microplates are needed. Sterile
polypropylene microplates areavailable upon request.
For colorimetric measurements in the visible wavelength range, transparent
polystyrene microplates are
ideal due to the high clarityof polystyrene. However, thetransmission rate of most solid

polystyrene vessels and plates
drops sharply at approximately 400 nm. The usage of thintransparent film bottoms inblack or white framed µClear®
plates extends detection capability down to 340 nm. Microplates with µClear® film
bottom are also an excellent
choice for standard microscopic applications (see also
chapter 3.5, p. 18-19).
For measurements in the lower
UV range, e.g. for the measurements of DNA or protein
concentration, UV-Star® film
bottom microplates manufactured entirely out of cycloolefin with transmission down to
230 nm are mandatory.

SUITABILITY OF MICROPLATES WITH REFERENCE TO WAVELENGTH

UV-Star®
film bottom
microplates

230 nmµClear®
film bottom
microplates

340 nmSolidbottommicroplates

400 nmwww.gbo.com

/ 13

3.3 IMMUNOLOGY
For assays based on the immobilisation of biomolecules
to the surface of microplates,
polystyrene is by far the mostcommonly used base material.
Due to its chemical nature,polystyrene is a hydrophobic
compound and non-treatedpolystyrene plates featurehydrophobic characteristics.
If attachment to the solid
surface is based upon passive
adsorption, e.g. in ELISA*,
physiochemical forces like hydrophobic bonds, hydrophilicinteractions and H-bonding
are relevant. Therefore, ELISA
microplates are most often
physically treated to introduce
a defined number of hydrophilic groups to the microplatesurface.

Greiner Bio-One offers both amedium-binding and a highbinding surface for passive
adsorption. The high-binding
surface features a relatively
high number of polar groups,
whereas the number of polargroups is limited on the medium-binding surface. The determination of which surface
is best suited for a specific application should be evaluatedempirically, as, in addition tosurface properties, it is important to consider issues such as
non-specific binding and otherassay parameters to make theappropriate selection.

* ELISA = Enzyme-linked Immunosorbent Assay
FIA = Fluorescence Immunoassay
LIA = Luminescence Immunoassay

14 /www.gbo.com

96 well ELISA strip plates

IMMUNOLOGY

For some applications, adsorptive binding to a physicallymodified polystyrene surface
is not feasible. One alternative is to take advantage ofthe strong non-covalent interaction between streptavidin
and biotin. Here, streptavidin-coated microplates act
as solid surface, upon which
biotinylated biomolecules canbe attached very effectively,
enabling a robust tool for microplate binding assays.

Microplates with a functional 3-dimensional matrix assurface offer the possibility forcovalent binding of biomolecules to the microplate surface.
Coupling can take place instandard coating buffers andneeds no additional steps.
Due to the nature of the 3-dimensional functional matrix,
non-specific background isvery low, and, in comparison tophysically treated microplates,the 3D matrix enhances signal
intensity.

IMMUNOLOGY MICROPLATESMEDIUM-BINDING

HIGH-BINDING

Low number of polar groups

High number of polar groups/

Medium binding capacity/

High binding capacity/

Clear microplates(MICROLON 200): ELISA/

Clear microplates(MICROLON 600): ELISA/Black microplates(FLUOTRAC 200): FIA/

Black microplates(FLUOTRAC 600): FIA/

White microplates(LUMITRAC 200): LIA/

White microplates(LUMITRAC 600): LIA

STREPTAVIDIN-BIOTIN
BINDING

Binding of biontinylated
molecules/

For binding of biotinylated
targets/

High chemical stability/

Transparent, black andwhite pigmentation

www.gbo.com

/ 15

3.4 STORAGE PLATES
Traditionally, microplates used
for storage of active reagents,
patient samples or biomolecules are made of polypropylene (see also chapter 3.2,
p. 12-13). Storage plates arecharacterised by biologicalinertness, resistance to numerous solvents, e.g. DMSO, anda wide range for temperature
resistance. MASTERBLOCK®
storage plates feature as wellelevated well walls to facilitate sealing. The footprint iscompatible with automatedsystems.

Polypropylene storage plates
are available from the 96
to the 1536 well format andwith U- and V-bottom welldesign. The 384 Deep Well
MASTERBLOCK® extends therange of polypropylene storage
plates. Its conical well shapeenables precise pipettingwith almost no dead volume inparallel with a maximised wellvolume. Therefore the Deep
Well MASTERBLOCK® is theideal solution for the storage ofcompound libraries.

Polypropylene(PP)

Cycloolefin(COC/COP)

Polystyrene(PS)

Temperature stability







Breakage resistance







Chemical resistance







Transparency







Material resistance of polypropylene and cycloolefinin comparison to polysytrene.

16 /www.gbo.com

STORAGE

Special demands on storage
plates are made by acoustic
liquid handling applications.
Therefore Greiner Bio-One‘scompound storage platesmeant for acoustic liquidhandling are subject to stringent production specifications
to ensure constant well bottomfeatures. These microplates
are deionised after production
and packed in antistatic bags.
Beside a 384 well polypropylene storage plate, Greiner
Bio-One offers a range of

cycloolefin storage plates foracoustic liquid handling in the
384 well and 1536 well format.
Cycloolefin combines many
utile features: resistance topolar solvents like DMSO, highoptical clarity and glass-like
optical properties, excellent
water and vapour barrier functions to minimise evaporation,nearly no leaching extractables
and low biomolecule binding.

STORAGE MICROPLATES

R1

R2

Polypropylene Microplates /MASTERBLOCK®

Cycloolefin Microplates/Compound storage/

Compound storage/

Sample collection/

Acoustic liquid handling/

Sample mixing/

Sample preparation/

Transport/

Screening/

Acoustic Liquid Handlingwww.gbo.com

/ 17

3.5 MICROSCOPY AND HIGH-CONTENT SCREENING
New
applications
inhigh-throughput and highcontent screening, as well as
high-resolution and confocal
microscopy, have increased
the demand for microplateswith pigmented walls and clear
bottom. The product portfolioof Greiner Bio-One contains
clear bottom microplates
either with glass or a highquality film bottom.
µClear® film bottom microplates combine a pigmented
frame with a transparent
bottom, a prerequisite for luminescence and fluorescenceapplications where bottomreading or microscopy are
involved. Due to the optimised
thickness of the film, the18 /www.gbo.com

intrinsic
autofluorescenceof polystyrene is minimised.
Black and white µClear® microplates are available both
non-treated and with a wide
variety of surface properties
and coatings well-suited forstandard detection and microscopic applications.
SCREENSTAR
microplateswith cycloolefin film bottom
are optimised for the specialised
requirementsofhigh-content screening andhigh-resolution microscopy.
The 190 µm cycloolefin film
bottom guarantees maximum
resolution, even at high microscopic magnification, andthe physical surface treatment
assures a proven performanceMICROSCOPY

for consistent cell attachment.
SCREENSTAR microplates provide a recessed bottom toallow full use of high-magnification oil or water immersion
objectives with access to all
microplate peripheral wells,
including perimeter and corner
positions
CELLview glass bottom microplates are designed for demanding and high-resolution
microscopic applications. They
consist of a cycloolefin-based
black frame with a 0.17 mmthin borosilicate glass bottom

providing superior images of
in-vitro cultures. Analogous to
the SCREENSTAR microplates,
the geometry is optimised forhigh-resolution
microscopythat allows imaging with shortworking distance. The roundand conical well design reduces
the meniscus effect in order
to assure equal cellular distribution and constant imaging
results. An appropriate surface
treatment improves cellular attachment and growth.

MICROPLATES FOR MICROSCOPYBASIC
MICROSCOPICAPPLICATIONSµClear® microplates(190 µm polystyrene
fi lm bottom)//

Optimised forstandard microscopy
with low to medium
magnification
Available withvarious tissue
culture surfaces

ADVANCED MICROSCOPIC APPLICATIONSCELLview cell culture microplates(175 µm glass bottom)

SCREENSTAR microplates(190 µm cycloolefi n fi lm bottom)/

For high-resolution microscopy//

Reduced well bottom elevation
optimsed for low working distances

For complex microscopic
applications in high-content
screening (HCS)/Maximal planarity//

Best optical properties

Reduced well bottom
elevation optimised for
low working distances/

Excellent optical properties/

Available with various tissue
culture surfaces

SensoPlate glass bottom microplates(175 µm glass bottom)/

For fluorescence correlation
spectroscopy, confocal microscopy
and single molecule detection/

Best optical properties/

Untreated glass surfacewww.gbo.com

/ 19

4. LITERATURE ABOUT MICROPLATESThis chapter gives you an
overview of our publications,application notes and reports
as well as our Greiner Bio-One
Forum issues and brochures
about microplates. All documents are published as pdf files

on our website. Just search forthe respective article numberin the search function of thedownload center. You can alsoorder a printed copy via e-mailto info@de.gbo.com.

Greiner Bio-One Forum
Language: english
Item No.

20 /

Title

Products

F071104

Advanced TC: An innovative surface improving cellular assays

Advanced TC

F073777

CELLSTAR® cell culture vessels with cell-repellent surface

Cell-repellent

F07312196 well half area microplates and their application in fluorescence,luminescence and transmission measurements

Half Area

F073120

SCREENSTAR: A 1536 well microplate for high-content and high-throughput
screening

SCREENSTAR

F073787

SCREENSTAR and CELLview: microplates for advanced microscopy

SCREENSTAR,
CELLview

F073000

A 384 well storage plate reducing compound consumption and supporting
assay miniaturisation

Storage plates

F073795

1536 well cycloolefin microplate for compound storage and acoustic liquidhandling

Storage plates

F073004

Microplates for Enzyme Linked Immunosorbent Assays (ELISA)

ELISA

F073013

Sealers for microplates and their areas of application in molecular biology andcell culture

Sealers

www.gbo.com

Link to ourdownload center

Application Notes
Language: english
Item No.

Title

Products

F010003

Selection of cell culture surfaces for the adipogenic differentiationof human mesenchymal stem cells (hMSC)

CELLSTAR® TC,
CELLCOAT®, Adv. TC

F071105

siRNA dependent gene silencing in HeLa cells cultivated on various cell culture
surfaces

CELLSTAR® TC,
CELLCOAT®, Adv. TC

F073117

Improved cultivation and differentiation of embryonic stem cells

CELLSTAR® TC,
CELLCOAT®, Adv. TC

F073022

Influence of washing steps on cell attachment: Comparison of PDL-coated andcell culture treated microplates

CELLSTAR® TC,
CELLCOAT®

F073041

UV/VIS spectroscopy in microplates UV-Star®, µClear®, MICROLON andCELLSTAR®

CELLSTAR® TC,
ELISA, UV-Star®,µClear®

F073106

Insulin ELISA on high binding MICROLON 600 and CELLSTAR® microplates

CELLSTAR® TC,
ELISA

F073113

Cultivation and differentiation of human adipose derived mesenchymal StemCells with CELLSTAR® and CELLCOAT® cell culture products

CELLSTAR® TC,
CELLCOAT®

F074058

Establishing a cell culture assay based on time-resolved fluorescenceresonance energy transfer (TR-FRET) for screening G-Protein coupled
receptors

CELLSTAR® TC

F073103

Enhanced transfection efficiency on protein coated microplates

CELLCOAT®

F073118

Influence of coating buffer and incubation conditions on ELISA performance

ELISA

F076036

Advanced TC: A cell culture surface improving the cultivation anddifferentiation of embryonic stem cells

Advanced TC

F073797

CELLSTAR® microplates with cell-repellent surface as platform for
BIOMIMESYS®, a new generation of a mimetic hydrogel for 3D cell culture

Cell-repellent

Brochures
Language: english
Item No.

Title

Products

F071067

Cell culture

Cell culture vessels

F071076

3D cell culture

3D cell culture

F073917

Intelligent solutions for sample storage

Storage plates

F073788

High-quality consumables and accessories for sample tracking and storage

Storage plates

www.gbo.com

/ 21

5. BARCODE SERVICE FOR MICROPLATES
5.1 GENERAL INFORMATION
Eliminating the use of barcodesfor sample tracking and sample
management in today’s routine
work in pharma research anddiagnostics is unthinkable,
given the significantly increasing amounts of data.
Barcode systems simplify and
expedite work processes. In
addition, they permit the unequivocal identification oflabelled samples at any timeand help minimise errors due
to sample mix up in manual
data collection.

Greiner Bio-One offers a comprehensive barcode service
for all 96, 384 and 1536 well
microplates. In an automated production process, labels
imprinted with barcodes aremounted on the outside rimsof the microplates. The typeof barcode used, the barcode
sequence, the labelling aswell as the position of thebarcode are all specified by thecustomer. The barcode labels
used are temperature-resistant (-70 °C to +50 °C). The label
and the barcode imprint aresmear-resistant and stable tonumerous solvents.

F073015
Ordering form forbarcoded microplates

22 /www.gbo.com

5.2 BARCODE ORDERINGPROCEDURE
The complete and detailed
filling out of our barcode
order form is the basis for theerror-free and fast barcode
service which we wish toprovide to our customers.
Microplates withlinear barcode

1

2

3

ORDER FORM
You will find the barcode
order form on our homepage
in the download center(F073015) or you may contact
your sales representative atGreiner Bio-One for a printed
copy.

SIGNATURE
After completely filling out
the form, please verify thecorrectness of all your information with your signature.

INTERNAL CHECKHaving received the completely filled out and signed
barcode order form, Greiner
Bio-One will check the feasibility of the requested
barcode. A customer-specific item number is assigned tothe order and communicatedto you by your sales representative at Greiner Bio-One,
along with an expected
delivery date.

4

5

SAMPLE PLATES
If desired and in consultation with your representative at Greiner Bio-One,
prototype specimen plateswith barcode can first be
produced as free samples.

REORDERING
Please indicate the desired
numbering sequence begin
and the desired sequence
end on your order. Only
written orders can be
accepted. If you have altered
the general barcode requirements for your plates in thereorders (e.g. a different
barcode type, a different labelling), we request that you
fill out a new barcode orderform.

www.gbo.com

/ 23

﻿Cell Culture Microplates

PRODUCT OVERVIEWADHERENT CELL CULTURE
CELLSTAR® TC
6 / 12 / 24 / 48 Well
Raw material: PS, Well profile: F-bottom / Chimney Well, Surface treatment: TC
Item No.

Well format

Product colour

BottomLid

SterileQty. inner / outer

657160

6

clear

solid

+*

+

1 / 100

665180

12

clear

solid

+*

+

1 / 100

662160

24

clear

solid

+*

+

1 / 100

677180

48

clear

solid

+*

+

1 / 100

CELLSTAR® TC
96 Well
Raw material: PS, Surface treatment: TC
WellformatProduct colour

SterileQty. inner /outer655160

96

clear

+

solid

+

1 / 100

655162

96

clear

+

solid

+

5 / 100

655180

96

clear

+

solid

+*

+

1 / 100

655182

96

clear

+

solid

+*

+

10 / 160

650160

96

clear

+

solid

+

1 / 100

650180

96

clear

+

solid

+

1 / 100

651160

96

clear

+

solid

+

1 / 100

651180

96

clear

+

solid

+

+

1 / 100

675180

96

clear

solid

+

+

8 / 32655079

96

black

+

solid

+

10 / 40655086

96

black

+

solid

+

8 / 32655087

96

black

+

µClear®

+10 / 40655090

96

black

+

µClear®

+*+8 / 32675086

96

black

+

solid

+

+

8 / 32675090

96

black

+

µClear®

++8 / 32655073

96

white

+

solid

+

10 / 40655083

96

white

+

solid

+

8 / 32655088

96

white

+

µClear®

+10 / 40655098

96

white

+

µClear®

+*+8 / 32675083

96

white

solid

+

+

8 / 32+* Lid with condensation rings
Well profile: for details see p. 39

24 /Well profile

Item No.

www.gbo.com

F/C

U

V

HA

+

+

Bottom

Lid

+

+*

+*

Cell Culture Microplates﻿

CELLSTAR® TC
384 WellRaw material: PS, Surface treatment: TC
Well profile
Item No.

Well format

Product colour

781165

384

clear

+

solid

781182

384

clear

+

solid

781079

384

black

+

solid

781086

384

black

+

solid

781092

384

black

+

µClear®

781091

384

black

+

µClear®

781090

384

black

+

784086384

black

781073

384

white

+

solid

781080

384

white

+

solid

781093

384

white

+

µClear®

781098

384

white

+

µClear®

784080

384

white

solid

F

SV
HiBase++BottomLid

SterileQty. inner / outer+

10 / 40+

8 / 32+10 / 40+

8 / 32+10 / 40++

8 / 32µClear®

++20 / 120

solid

+

+

8 / 32+10 / 40+

8 / 32+10 / 40++

8 / 32++8 / 32+++

CELLSTAR® TC
1536 Well
Raw material: PS, Well profile: F-bottom HiBase, Surface treatment: TC
Item No.

Well format

Product colour

BottomLid

SterileQty. inner / outer

782180

1536

clear

solid

+

+

1 / 32782078

1536

black

solid

+

15 / 60

782086

1536

black

solid

+

10 / 40782092

1536

black

µClear®

+15 / 60

782073

1536

white

solid

+

15 / 60

782080

1536

white

solid

+

10 / 40782093

1536

white

µClear®

+15 / 60++

www.gbo.com

/ 25

﻿Cell Culture Microplates

PRODUCT OVERVIEWADHERENT CELL CULTURE
CELLCOAT® Protein Coating
6 / 24 / 96 Well
Raw material: PS, Well profile: F-bottom / Chimney Well, Surface treatment: CELLCOAT®
Item No.

Well format

Product colour

Bottom

657940

6

clear

solid

657930

6

clear

solid

657950

6

clear

solid

662940

24

clear

solid

662930

24

clear

solid

662950

24

clear

solid

655940

96

clear

solid

655930

96

clear

solid

655950

96

clear

solid

65594696

blackµClear®

65594896

blackµClear®

65593696

blackµClear®

65595696

blackµClear®

65594496

whiteµClear®

Protein coating

Lid

Qty. inner /
outer

+*

5 / 50+*5 / 50+*5 / 50+*5 / 50+*5 / 50+*5 / 50+*5 / 20+*5 / 20+*5 / 20++*5 / 20++*20 / 120+*

5 / 20+*5 / 20+*5 / 20PDL

PLL

Coll. I

+
+
+
+
+
+
+
+
+

+
+
+

CELLCOAT® Protein Coating
384 / 1536 Well
Raw material: PS, Surface treatment: CELLCOAT®
Well profile

Item No.

Well
formatProduct colour

781940

384

clear

+

solid

781930

384

clear

+

solid

781950

384

clear

+

solid

781946

384

black

+

µClear®

781948

384

black

+

µClear®

781936

384

black

+

µClear®

781956

384

black

+

µClear®

784946384

black

781945384

white781944384

white782946

1536

black

F

SV HiBaseF HiBase+

BottomProtein coating

Lid

Qty. inner /
outer

+

5 / 20+5 / 20+5 / 20++5 / 20++20 / 120+

5 / 20+5 / 20PDL

www.gbo.com

Coll l

+
+
+

+
+

solid

+

+

5 / 20

+

solid

+

+

5 / 20+µClear®

++5 / 20µClear®

++5 / 20++* Lid with condensation rings
Protein coatings: PDL = Poly-D-Lysine; PLL = Poly-L-Lysine; Coll. l = Collagen Type l
Well profile: for details see p. 39

26 /

PLL

Cell Culture Microplates﻿

Advanced TC
Raw material: PS, Surface treatment: Advanced TC
Item No.

Well
formatProduct colour

6579606

clear

66598012662960Well profile

BottomLid

SterileQty. inner /
outer

+

solid

+*

+

1 / 100

clear

+

solid

+*

+

1 / 100

24

clear

+

solid

+*

+

1 / 100

677980

48

clear

+

solid

+*

+

1 / 100

655980

96

clear

+

solid

+*

+

1 / 100

655982

96

clear

+

solid

+*

+

10 / 160

655986

96

black

+

µClear®

+*+8 / 3267598696

blackµClear®

++8 / 3265598396

whiteµClear®

+*+8 / 3267598396

whiteµClear®

++8 / 32781986

384

black

+

µClear®

++8 / 32781983

384

white

+

µClear®

++8 / 32

F/C

HA

F

+
+
+

www.gbo.com

/ 27

﻿Cell Culture Microplates

PRODUCT OVERVIEWNON-ADHERENT CELL CULTURE
CELLSTAR® Suspension Culture
Raw material: PS, Surface treatment: suspension
Well profile

BottomLid

SterileQty. inner /
outer

+

solid

+*

+

1 / 100

clear

+

solid

+*

+

1 / 100

24

clear

+

solid

+*

+

1 / 100

677102

48

clear

+

solid

+*

+

1 / 100

655185

96

clear

+

solid

+*

+

1 / 60650185

96

clear

solid

+

+

1 / 60Item No.

Well format

Product colour

657185

6

clear

66510212662102F/CU+

CELLSTAR® Cell-Repellent Surface
Raw material: PS, Surface treatment: cell-repellent
Item No.

Well
formatProductcolour6579706

clear66597012662970BottomLid

SterileQty. inner /
outer

+

solid

+*

+

1/5

clear

+

solid

+*

+

1/5

24

clear

+

solid

+*

+

1/5

677970

48

clear

+

solid

+*

+

1/5

655970

96

clear

+

solid

+*

+

1/6

650970

96

clear

+

solid

+*

+

1/6

650979

96

clear

+

solid

+*

+

8 / 32651970

96

clear

solid

+*

+

1/6

655976

96

black

+

µClear®

+*+8 / 32655976-SIN

96

black

+

µClear®

+*+1 / 32781970

384

clear

solid

+

+

1 / 60787979

384

clear

solid

+

+

8 / 32781976

384

black

+

µClear®

++8 / 32781976-SIN

384

black

+

µClear®

++1 / 32781974

384

white

+

µClear®

++8 / 32+* Lid with condensation rings
Well profile: for details see p. 39

28 /Well profile

www.gbo.com

F

F/C

U

V

+

+
+

Screening Microplates﻿

SCREENING MICROPLATESNon-Treated Microplates (96 Well PS / PP)
Surface treatment: untreated, Lid: no
Raw material

Well profile

Item No.

Well
format655101

96

+

clear

+

solid

655161

96

+

clear

+

solid

650101

96

+

clear

+

solid

650161

96

+

clear

+

solid

651101

96

+

clear

+

solid

651161

96

+

clear

+

solid

675101

96

+

clear

+

solid

675161

96

+

clear

+

solid

655076

96

+

black

+

solid

10 / 40655096

96

+

black

+

µClear®

10 / 40675076

96

+

black

+

solid

10 / 40675096

96

+

black

+

µClear®

10 / 40655075

96

+

white

+

solid

10 / 40655095

96

+

white

+

µClear®

10 / 40675075

96

+

white

solid

10 / 4065520196+

naturalsolid

10 / 100

65020196+

natural+solid

10 / 100

65026196+

natural+solid

65120196+

natural655209

96

+

black

650209

96

+

black

651209

96

+

black

PS

PP

Product colour

F

F/C

U

U/C

V

V/C

HA

+
+

+
+
+
+

BottomSterileQty. inner/ outer

10 / 100+

2 / 100
10 / 100+

2 / 100
10 / 100+

2 / 100
10 / 40++

10 / 40

10 / 100solid

10 / 100solid

10 / 100solid

10 / 100solid

10 / 100

www.gbo.com

/ 29

﻿Screening Microplates

PRODUCT OVERVIEWSCREENING MICROPLATESNon-Treated Microplates (384 Well PS / PP)
Surface treatment: untreated
Raw material

Well profile

Item No.

Well
format781101

384

+

clear

781162384+

781185384

781186

Lid

+

solid

no

clear

+

solid

no

+

10 / 100

+

clear

+

solid

yes

+

1 / 32

384

+

clear

+

solid

yes

+

8 / 32784101

384

+

clear

solid

no

10 / 40781076

384

+

black

+

solid

no

10 / 40781096

384

+

black

+

µClear®

no10 / 40784076

384

+

black

+

solid

no

10 / 40784076-25

384

+

black

+

solid

no

25 / 150

781075

384

+

white

+

solid

no

10 / 40781095

384

+

white

+

µClear®

no10 / 40784075

384

+

white

+

solid

no

10 / 40784075-25

384

+

white

+

solid

no

25 / 150

781201384+

naturalsolid

no10 / 100

781280384+

naturalsolid

no10 / 100

781209

384

+

black

solid

no

10 / 100

PPF

SV
HiBase

V

+

+
+
+

SterileQty. inner /outer

BottomPS

Product colour

10 / 100

Non-Treated Microplates (1536 Well PS / PP)
Surface treatment: untreated, Lid: no
Raw material

Item No.

Well
formatPS

7821011536

782076

+

clear

+

solid

15 / 60

1536

+

black

+

solid

15 / 60

782096

1536

+

black

+

µClear®

15 / 60

782075

1536

+

white

+

solid

15 / 60

782095

1536

+

white

+

µClear®

15 / 60

7822701536+

natural+solid

15 / 60

782261

1536+

natural+solid

www.gbo.com

PP

V
Deep Well

BottomSterileQty. inner /outer

F
HiBaseWell profile: for details see p. 39

30 /Well profile
Product colour+

15 / 60

Screening Microplates﻿

High-Binding Microplates (sterile)
Raw material: PS, Surface treatment: high-binding, Lid: no
Well profile

Item No.

Well
format65507796

black65509796

black

67507796

black

65507496

white65509496

white67507496

white781061384

clear

781077384

781097

Product colour

BottomSterileQty. inner / outer

+

solid

+

10 / 40+µClear®

+10 / 40solid+

10 / 40

+

solid

+

10 / 40+µClear®

+10 / 40solid+

10 / 40

+

solid

+

10 / 40

black

+

solid

+

10 / 40

384

black

+

µClear®

+10 / 40781074

384

white

+

solid

+

10 / 40782061

1536

clear

+

solid

+

15 / 60

782077

1536

black

+

solid

+

15 / 60

782097

1536

black

+

µClear®

+15 / 60

782074

1536

white

+

solid

+

15 / 60

F

F/C

HA

F
HiBase++www.gbo.com

/ 31

﻿Screening Microplates

PRODUCT OVERVIEWSCREENING MICROPLATES
Non-Binding Microplates (96 / 384 / 1536 Well)
Raw material: PS, Surface treatment: non-binding, Lid: no
Well profile

Item No.

Well
formatProduct colour

65590196

clear

65090196

clear65190196

clear65590096

black

65590696655904

F

F/C

U

V

SV
HiBaseF
HiBase+BottomSterileQty. inner /
outer

solid

10 / 40solid

10 / 40solid

10 / 40+solid

10 / 40

black+µClear®

10 / 40

96

white

+

solid

10 / 40655903

96

white

+

µClear®

10 / 40781901

384

clear

+

solid

10 / 40781900

384

black

+

solid

10 / 40781906

384

black

+

µClear®

10 / 40784900

384

black

solid

10 / 40781904

384

white

+

solid

10 / 40781903

384

white

+

µClear®

10 / 40784904

384

white

solid

10 / 40782900

1536

black

+

solid

15 / 60

782904

1536

white

+

solid

15 / 60++++

UV-Star® Microplates (96 / 384 Well)
Raw material: COC, Surface treatment: untreated, Lid: no
Item No.

Well format

65580196

clear67580196

clear781801384

clear

Well profile: for details see p. 39

32 /www.gbo.com

Product colour

Well profile
F

F/C

HA

+
+
+

BottomSterileQty. inner / outer

Cycloolefin film

10 / 40

Cycloolefin film

10 / 40

Cycloolefin film

10 / 40

Immunology Microplates﻿

IMMUNOLOGY MICROPLATESStandard ELISA-Microplates (medium- / high-binding)Well format: 96, Raw material: PS, Bottom: solid, Lid: no
Item No.

Well format

Productcolour

65500196

clear

65508096

clear

65000196

clear65100196

clear67500196

clear

65506196

clear

65508196

clear

65006196

clear65106196

clear

675061

96

clear

Bindingcharacteristic

Well profile
F

F/C

U

V

HA

+
+
+
+
+

Binding
Brand name

Qty. inner /
outer

+

MICROLON 200

10 / 40+

MICROLON 200

10 / 40+

MICROLON 200

10 / 40+

MICROLON 200

10 / 40+

MICROLON 200

10 / 40+

MICROLON 600

10 / 40+

MICROLON 600

10 / 40+

MICROLON 600

10 / 40+

MICROLON 600

10 / 40+

MICROLON 600

10 / 40

med.

+
+
+
+
+

high

ELISA Strip Plates (medium- / high-binding)Well format: 96, Raw material: PS, Bottom: solid, Lid: no
Item No.

Strip plate design
6x18

Product colour

12x8

Well profile

Bindingcharacteristic

F

med.

U

756070

+

clear

+

756071

+

clear

+

754070

+

clear

+

754061

+

clear

+

high

+
+

762070

+

clear

+

762071

+

clear

+

767070

+

clear

+

767071

+

clear

+

762076

+

black

+

762077

+

black

+

762075

+

white

+

762074

+

white

+

+
+
+
+
+
+
+
+
+
+

Binding
Brand name

Qty. inner / outer

MICROLON 200

10 / 100

MICROLON 600

10 / 100

MICROLON 200

10 / 100

MICROLON 600

10 / 100

MICROLON 200

10 / 100

MICROLON 600

10 / 100

MICROLON 200

10 / 100

MICROLON 600

10 / 100

FLUOTRAC 200

10 / 100

FLUOTRAC 600

10 / 100

LUMITRAC 200

10 / 100

LUMITRAC 600

10 / 100

www.gbo.com

/ 33

﻿Immunology Microplates

PRODUCT OVERVIEWIMMUNOLOGY MICROPLATESSingle-Break Strip Plates (medium- / high-binding)Well format: 96, Well profile: C-bottom, Raw material: PS, Bottom: solid, Lid: no
Strip plate
designProduct colour

705070

12x8

clear

705063

12x8

clear

70506512x8705066

Colour coding
well rim

Bindingcharacteristic

Binding
Brand name

Qty. inner / outer+

MICROLON 200

10 / 100red+

MICROLON 200

10 / 100

clear

green

+

MICROLON 200

10 / 100

12x8

clear

yellow+

MICROLON 200

10 / 100

705071

12x8

clear

+

MICROLON 600

10 / 100

705073

12x8

clear

red

+

MICROLON 600

10 / 100

705074

12x8

clear

blue

+

MICROLON 600

10 / 100

705075

12x8

clear

green

+

MICROLON 600

10 / 100

705076

12x8

clear

yellow+

MICROLON 600

10 / 100Item No.

med.

high

Streptavidin-Coated Microplates
Raw material: PS, Bottom: solid, Surface treatment: Streptavidin, Lid: no
Product colour

Well profile

Item No.

Well format

655990

96

clear

+

5 / 40655997

96

black

+

5 / 40655995

96

white

+

5 / 40781990

384

clear

+

5 / 40781997

384

black

+

5 / 40781995

384

white

+

5 / 40C

F

Qty. inner / outer

Covalent-Binding Microplates
Microplates with a covalent binding surface can be ordered on request. Please contact your sales representative formore information.

Well profile: for details see p. 39

34 /www.gbo.com

Storage Plates﻿

STORAGE PLATESPolypropylene Microplates (96 Well)
Well format: 96, Raw material: PP, Bottom: solid, Lid: no
Product colour

Well profile

Item No.

Well format

65520196

natural

65020196

natural+65026196

natural+65120196

natural65520996black

65020996black651209

96

black

F/C

U/C

SterileV/C

Qty. inner / outer+

10 / 100
10 / 100+

10 / 100+

10 / 100+

10 / 100+

10 / 100+

10 / 100

Polypropylene Microplates (384 / 1536 Well)
Raw material: PP, Bottom: solid, Lid: no
Well profile
Product colour

V
Deep
Well

Item No.

Well format

781201384

natural781280384

natural781270384

natural+781271384

natural+

784201384

natural781209384

black

7822701536

natural+782261

1536

natural

+

F

V

SV
Deep
Well

Sterile+Qty. inner / outer

10 / 100+

10 / 100
6 / 60++6 / 6010 / 100+

10 / 100
15 / 60+

15 / 60

www.gbo.com

/ 35

﻿Storage Plates

PRODUCT OVERVIEWSTORAGE PLATES
Cycloolefin Microplates (for Acoustic Liquid Handling)
Raw material: cycloolefin, Bottom: solid, Sterile: no, Lid: no
Raw material

Item No.

Well
format781201-906

384

793855

384

+

clear

782855

1536

+

clear

792870-906

1536

+

clear

PP

COC

+

Well profile

ProductcolourF

natural+F
HiBaseSterileSV
HiBaseQty. inner / outer

10 / 100+

15 / 60+

15 / 60+

15 / 60

Polypropylene MASTERBLOCK®
Raw material: PP, Bottom: solid, Lid: no
WellformatProduct colour

78620196

natural+

0.5 ml78626196

natural+

0.5 ml78020196

natural+

1 ml

1 / 5078021596

natural+

1 ml

5 / 5078026196

natural+

1 ml

78027096

natural+

2 ml

1 / 5078028596

natural+

2 ml

5 / 5078027196

natural+2 ml

Well profile: for details see p. 39

36 /Well profile

Item No.

www.gbo.com

UV

Description

SterileQty. inner / outer8 / 80+++1 / 801 / 501 / 50Microplates for Microscopy﻿

MICROPLATES FOR MICROSCOPY
Glass-Bottom Microplates
Raw material: PS, Bottom: glass, Lid: yes, Sterile: yes
Item No.

Well format

Productcolour66289224

black65589296655891

Well profile
Surface treatment

SterileQty. inner /
outer

+

untreated+

1 / 12black+

untreated+

1 / 16

96

black

+

TC

+

1 / 16655981

96

black

+

Advanced TC+

1 / 16781892384

black

untreated+

1 / 16783892

1536

black

untreated+

1 / 16F

F
LoBaseF/C++

SCREENSTAR Microplates with Cycloolefin Film Bottom
Raw material: COP, Bottom: cycloolefin film, Surface treatment: TC, Sterile: yes
Item No.

Well format

Product colour

65586696

black

781866384

black

789866

1536

black

Well profile
F/C

Surfacetreatment

LId

SterileQty. inner / outer

TC

+

+

1 / 16

+

TC

+

+

8 / 32+TC+

17 / 68

F+µClear® Film Bottom MicroplatesBlack and white µClear® microplates are available both non-treated and with a wide variety of surface properties andcoatings well-suited for standard detection and microscopic applications.

www.gbo.com

/ 37

﻿Lids / Sealers / CapMats

PRODUCT OVERVIEW
LIDS / SEALERS / CAPMATS
Lids
Raw material: PS
Item No.

Height

Lid type

Condensation rings

6561019 mm

high6561619 mm

high656170

9 mm

high

+

656171

9 mm

high

+

6561906 mm

flat

656191

6 mm

flat

691101

4.8 mmultra low

691161

4.8 mmultra low

SterileQty. inner / outer1 / 100+

1 / 100
1 / 100+

1 / 100
20 / 200+

20 / 200
5 / 100+

5 / 100

SterileQty. inner / outer

Sealers
Item No.

Description

FeaturePierceable

676001EASYsealclear

676090SILVERseal

aluminium foil

676070VIEWsealclear

100 / 1,200

676040AMPLIsealclear

100 / 2,000

676050BREATHseal

gas permeable

50 / 2,500

676051

BREATHseal

gas permeable

100 / 2,000+

100 / 1,200+

50 / 500

Cap Mats
Description: 96 Well CapMat, Pierceable: no, Raw material: EVA

38 /Item No.

Nap shape

381070round

381061round

381080square381081squarewww.gbo.com

SterileQty. inner / outer

10 / 50+

1 / 5010 / 50+

1 / 50WELL DESIGNSOF GREINER BIO-ONE MICROPLATES

96 WELL MICROPLATES
F

U

V

C

HA

F-BottomU-Bottom

V-Bottom

C-BottomHalf Area

For precise opticalmeasurements
and microscopic
applications(bottom reading)For easy andresidue-freepipetting

For precise
pipetting andsample storage

Flat-bottom
profile withrounded corners

An alternative
to the use ofhigh-format
microplates due
to a reductionof the sample
volume of up
to 50 %For residue-freepipetting andprecise opticalmeasurements

Chimney Well*/C

The standard microplate well has the same profile as the chimney well. The
difference from the standard plate is the chimney-like arrangement of the wells.
Each well stands on its own. Therefore the risk of sample carryover and crosscontamination is minimised.

384 WELL MICROPLATESF

1536 WELL MICROPLATESSV HIBASEF HIBASE

F-Bottom

Small Volume HiBase

F-Bottom HiBase

For precise optical measurements and microscopic
applications (bottom reading)/

Top reading even at low
working volumes/

For top reading even at low
working volumes/

Savings in reagents similar
to 1536 well//

For transmission/
fluorescence/luminescenceapplications

For transmission/
fluorescence/luminescenceapplications/

Excellent optical properties/

Excellent optical properties

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/ 39

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F073048 en [rev. 05.2022]

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