Food and Enviromental TestingiQ-Check™ Real-Time PCR Solution
Real-Time PCR Methods for the Detection of Food and Environmental Pathogens

Visit bio-rad.com/FoodScience for more information.

The iQ-Check Solution
Providing Peace of Mind for Food Safety TestingF

Based on PCR gene amplification and real-time detection,
iQ-Check Kits use DNA primers and fluorescent probes specific to
the target organism (Figure 1). Detection of a fluorescent signal for theamplified target sequences indicates the presence of the pathogen
in the sample being tested. An internal positive control is included tovalidate any negative results in each well (PCR inhibitor monitoring).

QQFFig. 1. Illustration of the Bio-Rad Laboratories, Inc.
fluorescent probe.

Instruments and Software
iQ-Check Prep System■ Liquid handling platform for DNA extraction and PCR plate setup

iQ-Check Prep™

■

■

1

510!
152025

Completely integrated solution for pathogen detection with iQ-Check Kits
 ull sample traceability and bidirectional laboratory information management
Fsystem communication

CFX Opus Deepwell Real-Time PCR System■ Delivers sensitive 5-color multiplex performance30■

Flexible connectivity for data management and instrument control■

Open platform

CFX Manager Software, Industrial Diagnostic Edition (IDE)■ Easy-to-use sample setup

pwells Dee
Systemime PCR
CFX OpuReal-T■

Secure and customizable reports■

Ability to use open software for other laboratory tests

Validations
iQ-Check Method

NF Validation by
AFNOR Certification

AOAC

••

Escherichia coli O157:H7••

Listeria monocytogenes••

Listeria spp.

••

•

•

Salmonella Enteritidis

•

OMA*

Salmonella Typhimurium•STEC 7 major O-groups•STEC virulence genes•

Vibrio•2 | BIO-RAD LABORATORIES, INC.

•MicroVal•

Enterobacteriaceae

STEC, Shiga toxin–producing E. coli.
* Official Method of Analysis First Action, 2017.06.

Health Canada•Campylobacter spp.

Salmonella spp.

NordVal•Aspergillus

Cronobacter spp.

RESEARCH
INSTITUTE•••

Easy-to-Use Protocols
Manual Solution: Four-Step Protocol

Automated Solution: iQ-Check Prep System Workflow

1. Enrich sample.

1. Load enriched samples
on an iQ-Check Deep Well
Microplate or in tubes
according to thesample worklist.

2. Extract DNA:

2. Load consumables,

collect enriched sample
and lyse cells.

iQ-Check Prep™

reagents, and samples
and run the iQ-CheckPrep System.
1

3. Set up PCR run:

510!
15202530

3. Run real-time PCR

add extracted DNA
to PCR wells.

432

1

5

6

7

8

910

11and get your results.

12A
B
C

wellDeepSysteme PCR
CFX Opus
Real-Tim

DEFGH

4. Run real-time PCR

Perform up to 480 tests in a single 8 hr shiftusing one iQ-Check Prep and one
real-time PCR system.

and get your results.
wellDeepSysteme PCR
CFX Opus
Real-TimApplications
iQ-Check methods can be used as a powerful alternative to standard reference methods for thedetection of food and environmental pathogens.
■■■ afely remove free DNA with our nontoxic, easy-to-use
SiQ-Check Free DNA Removal Solution
Use PIF Supplement to harmonize enrichment forEnterobacteriaceae, Cronobacter, and Salmonella in milk
powder, infant formula, cereal, and raw flour
Screen for Listeria spp., Salmonella, or other targets as part ofyour environmental monitoring program or HACCP plan

■

■

■

■

 armonize enrichment for Salmonella and STEC detection inHraw meat
Detect threshold levels of Salmonella in poultry and rinse samples
Use the iQ-Check Purification Reagent treatment for difficult
matrices, such as spices, cocoa, or cannabis products ake advantage of the open software and system (geneticallyT
modified organism, allergen, or norovirus detection)

For a complete list of applications or to discuss how the iQ-Check System can be
integrated into your lab, contact your local Bio-Rad food safety specialist.

BIO-RAD LABORATORIES, INC. | 3

Ordering Information
Catalog #iQ-Check Kits
12010806
35781353578137357811412003068
3578124357811335781233578142
12013174
357813912004306
12006574

Description
iQ-Check Aspergillus Kit, 96 reactions
iQ-Check Campylobacter Kit, 96 reactions
iQ-Check Cronobacter Kit, 96 reactions
iQ-Check E.coli O157:H7 Kit, 96 reactions
iQ-Check Enterobacteriaceae Kit, 96 reactions
iQ-Check Listeria monocytogenes II Kit, 96 reactions
iQ-Check Listeria spp. Kit, 96 reactions
iQ-Check Salmonella II Kit, 96 reactions
iQ-Check S. Enteritidis Kit, 96 reactions
iQ-Check STEC SerO II Kit, 32 reactions
iQ-Check STEC VirX Kit, 96 reactions
iQ-Check S. Typhimurium Kit, 96 reactions
iQ-Check Vibrio Kit, 96 reactions

iQ-Design Assays
12015337
iQ-Design Assay Aspergillus flavus, 100 reactions
12015260
iQ-Design Assay Aspergillus fumigatus, 100 reactions
12015336
iQ-Design Assay Aspergillus niger, 100 reactions
12018492
iQ-Design Assay Aspergillus terreus, 100 reactions
12013770
iQ-Design Assay E. coli O104, 200 reactions
12015330
iQ-Design Assay Aspergillus Identification Positive Control
12018493
iQ-Design Assay Aspergillus + terreus Positive Control
Supplemental Reagents
3594970
iQ-Check Free DNA Removal Solution, 1 vial lyophilized
iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vialof iQ-Check 10x Activation Buffer (9 ml)
3578138
iQ-Check Lysis II Reagent
3578136
iQ-Check Lysis Beads
12012383
iQ-Check Purification Reagent, 1 vial to make 25 ml for 96 tests
12013322
PIF Supplement, 2 g
Media for Sample Enrichment
3564684
Buffered Peptone Water Plus, dehydrated, 500 g
3554179
Buffered Peptone Water Plus, 225 ml x 6 bottles
3555795
Buffered Peptone Water Plus, 3 L x 4 bags
3555790
Buffered Peptone Water Plus, 5 L x 2 bags
12013259
Buffered Peptone Water Standard, dehydrated, 500 g
12013258
Buffered Peptone Water Standard, dehydrated, 5 kg
12013260
Buffered Peptone Water Standard, 5 L x 2 bags
3555703
Listeria Special Broth (LSB), 225 ml x 6 bottles
3564703
Listeria Special Broth (LSB), dehydrated, 500 g
3564753
Listeria Special Broth (LSB), dehydrated, 5 kg
12017463
Listeria Special Broth II (LSB II), 225 ml x 6 bottles
12017388
Listeria Special Broth II (LSB II), dehydrated, 500 g
12017378Listeria Special Broth II (LSB II), dehydrated, 5 kg
12007405
Vibrio Enrichment Broth, dehydrated, 500 g

Catalog #Description

Chromogenic Media for Confirmation
3564748
RAPID'E.coli O157:H7 Dehydrated Base, 100 g
3564744
RAPID'Listeria spp. Dehydrated Base, 500 g
3554012RAPID'Enterobacteriaceae Agar, ready to use, 200 ml x 6 bottles
3563694
RAPID'L.mono Agar, 90 mm x 20 plates
3563971
RAPID'Sakazakii Agar, 90 mm x 20 plates
3563963
RAPID'Salmonella Agar, 90 mm x 120 plates
3563695
AL (Agar Listeria according to Ottaviani and Agosti),
90 mm x 20 plates
Instruments and Software
17007991
CFX Opus Deepwell Real-Time PCR System, 96 wells
3600037
CFX96 Touch Deep Well Real-Time PCR System, 96 wells
3594911
iQ-Check Prep System
3593893
CFX Manager Software, Industrial Diagnostic Edition (IDE)
Consumables
HSP9655XTUHSP9955XTU
3594900
MLL9651XTU
TLS0851XTU
TCS0803XTU
2240110XTU
3593977

Hard-Shell™ 96-Well Skirted PCR Plates, pkg of 50, low profile,thin walled, white shell, white wells
Hard-Shell 96-Well Skirted PCR Plates, pkg of 50, barcoded,
low profile, thin walled, white shell, white wells
iQ-Check Deep Well Microplates, 96-well plates, 50
Multiplate 96-Well PCR Plates, low profile, unskirted, pkg of 25
0.2 ml 8-Tube PCR Strips without Caps, pkg of 120, low profile,thin walled, white
Optical Flat 8-Cap Strips, pkg of 120, for 0.2 ml tubes and plates,
ultraclear
Conical Tubes, pkg of 500, 1.5 ml, with installed O-ringscrewcaps, sterilized
X-Pierce Films, pkg of 100

Visit our website for more information and to see how our productscan be incorporated into food testing workflows:
iQ-Check Real-Time PCR Kits — bio-rad.com/iQCheck
RAPID'Chromogenic Media — bio-rad.com/RapidMedia
BIO-RAD and HARD-SHELL are trademarks of Bio-Rad Laboratories, Inc. in certain
jurisdictions. IQ-CHECK is a trademark of Bio-Rad Europe GmbH in certain jurisdictions.
All trademarks used herein are the property of their respective owner.
© 2023 Bio-Rad Laboratories, Inc.

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