High-throughput DNA purification from Oragene/saliva samples†with the Magtration® 12GCP. Lem‡, J. Chartier‡, J. Saini**, and K. Obata††‡ DNA Genotek, Ottawa, Ontario, Canada** Cortex Biochem Inc., San Leandro, CA†† PSS Bio Instruments Inc., Pleasanton, CA, USA
2006-06-29

The Magtration®12GC is an automated high-throughput
DNA purification system that uses paramagneticparticle technology. The 12GC can purify up to twelve
Oragene®/saliva samples in 30 minutes, with excellent
DNA yields.

Introduction
Large-scale population studies may involve thecollection of thousands of patient samples. Manual
purification of DNA from these samples can betime- and labor-intensive. The Magtration 12GC
from PSS Bio Instruments is an automated DNA
purification robot that uses pre-filled MagaZorb®
cartridges for fast and convenient DNA purification.
The purpose of this study was to determine
the DNA yield of saliva samples collected withOragene and processed with the 12GC.

card with the DNA Common-200 protocol wassupplied with the instrument. The elution volume
was 200 µL.

DNA analysis
Purified DNA was quantified by absorbance at 260 nm.
The A260/280 ratio was also determined.

Results
Figure 1 shows DNA yields for the 20 Oragene/saliva samples. The median DNA yield was 3.8 µg
per 200 µL of starting sample and the medianA260/280 ratio was 1.95.
987DNA collection

6Oragene self-collection kits (containing 2 mL ofOragene solution) were used to collect 2 mL of saliva
from 20 donors. Prior to purification with the 12GC,
the Oragene/saliva samples were incubated overnightat 50°C.

DNA yield (µg)

Materials and methods

5432

Automated DNA purification

1DNA was extracted from 200 µL of each Oragene/saliva sample using MagaZorb DNA common kit-200
cartridges on the 12GC system. An integrated circuit0†

Figure 1: Scattergram of DNA yields from 200 µL of Oragene/saliva
sample. The horizontal line represents the median yield — 3.8 µg.

Saliva samples were collected with Oragene®•DNA or Oragene®•DISCOVER.

(outside the USA)(in the USA)

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Discussion and conclusions
The 12GC robot can purify PCR-quality DNA fromwhole blood, bacteria, viruses, and tissues. From whole
blood, the expected DNA yield is 2-4 µg/100 µL, withan A260/280 ratio of 1.75 to 1.951. The median yieldof 3.8 µg/200 µL for the Oragene/saliva samples
compares favorably to the values for blood,considering that the saliva sample is diluted 1:1 with
Oragene solution in the collection vial. The medianA260/280 ratio of 1.95 indicates that the 12GC is
effective at removing protein contaminants.
To purify the entire 4 mL Oragene/saliva sample atonce, the Magtration System 8Lx may be used insteadof the 12GC. The 8Lx uses similar magnetic particle
technology as the 12GC and can process sample
volumes up to 7 mL.
In summary, the 12GC robot can purify up to twelve
Oragene/saliva samples in 30 minutes, with excellent
DNA yields.

References
1

Magtration®-MagaZorb® DNA common kit-200 protocol. PSS Bio Instruments. Revision 2.0.

Oragene®•DNA is not available for sale in the United States.
Oragene®•DISCOVER is for research use only, not for use in diagnostic procedures.
®Oragene is a registered trademark and prepIT™ is a trademark of DNA Genotek Inc. All other brands and names contained herein are the property of their respective owners.
All DNA Genotek protocols, white papers and application notes, are available in the support section of our website at www.dnagenotek.com.

MK-AN-007 Issue 3/2011-12© 2011 DNA Genotek Inc., a subsidiary of OraSure Technologies, Inc., all rights reserved.

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