Droplet Digital PCR

QX200™ Droplet Digital™ PCR System

IT STARTS WITH A

DROPLETAND ENDS INDISCOVERY.

Droplet Digital PCR (ddPCR™) is Bio-Rad’s unique digital PCR technology. With unrivaled precision,
Droplet Digital PCR provides absolute quantification of target DNA or RNA molecules without theuse of standard curves. It addresses the lack of scalable and practical technologies for digital PCR
implementation. The new QX200 Droplet Digital PCR System puts this powerful technology in yourhands, ready to unveil new worlds of research at previously unattainable levels.
PCR becomes “digital” through sample partitioning and the subsequent statistical analysis oftarget detection across the partitions. The QX200 System uses advanced microfluidics technology
to achieve partitioning on a massive scale, generating 20,000 highly uniform nanoliter-sized droplets
per sample. For even higher sensitivity, it is possible to combine wells and analyze millions of droplets
for a single sample.
Simple and reliable, Droplet Digital PCR technology has already led to several research breakthroughs infields such as cancer biomarker discovery, infectious diseases, genomic alterations, and gene expression.
This research is only the beginning. What discoveries will you make using Droplet Digital PCR?

WHAT’S IN A DROPLET?

A VERSATILE ANDSCALABLEWORKFLOW

Droplet Digital PCR has a simple, user-friendly experiment setup designed to process
eight samples at a time. The process easily scales up to run a 96-sample experimentin 5 hours with minimal hands-on time. When higher throughput is required, multiple
96-sample experiments can be run in a day.
Bio-Rad’s QX200 ddPCR System combines water-oil emulsion droplet technology
with microfluidics. The QX200 System consists of two instruments — a droplet
generator and a droplet reader­— and associated consumables. The droplet generator
partitions each sample into 20,000 uniform nanoliter-sized droplets in which nucleic acidmolecules are distributed in a random fashion. Each droplet serves to partition thereactions. Droplets are transferred to a 96-well PCR plate and PCR is performed to
end point in a thermal cycler. The droplets stream single file through the reader for
fluorescence analysis. Positive droplets, which contain at least one copy of the target
DNA or RNA molecule, exhibit increased fluorescence compared to negative droplets.
The fraction of PCR-positive droplets enables the target to be quantified according
to the Poisson distribution.
The QX200 System works with both hydrolysis probes and EvaGreen fluorescence
detection chemistries, and its flexible design allows for high throughput andultra-sensitive detection.
KEY BENEFITS
Achieve absolute quantification without the use of a standard curve
Design scalable assays for high sensitivity or high throughput■■ 
E xpand applications using flexible ddPCR chemistry — EvaGreen or probes■■■■

Bio-Rad Laboratories, Inc.

Workflow

Sample1
Oil

Oil
Droplets

1

2

3 4 5 6 7
DG8™ Cartridge

Prepare ddPCR reaction mix

2■■

 ombine DNA sample, primers, and/or probesCwith one of Bio-Rad’s ddPCR Supermixes■■

 ully validated PrimePCR ddPCR Assays
F
can be used

Generate droplets

8

3

■■

 oad the ddPCR reaction mix into the wells ofLa droplet generator cartridge■■

 x 20,000 droplets are generated from each8
run in the QX200 Droplet Generator■■

 arget DNA (T) and background DNA (randomly distributed in droplets) are

Perform PCR with E vaGreenor hydrolysis probes

4■■ ransfer the droplets to a 96-well PCR plateTand seal the plate■■

Run the PCR protocol

Read and analyze results■■ fter PCR, load the 96-well PCR plate intoA
the QX200 Droplet Reader■■ ositive and negative droplets in eachPsample are read■■ nalyze concentrations withA
QuantaSoft™ Software

Visit bio-rad.com/QX200 for more information.

WHAT’S IN A DROPLET?

QUANTIFYINGBIOMARKERS FORCANCER

The level of sensitivity offered by the QX200 ddPCR System in quantifying cancer biomarkers
overcomes the limitations posed by other methods. Using Droplet Digital PCR technology,
researchers are now able to observe finer quantitative distinctions among mutations, better
identifying their potential roles in cancer.
Cancer-associated mutations often evade detection due to their low concentrations relative
to the background of wild-type DNA in a given sample. With its high sensitivity, the QX200
System can easily scale to quantify target concentrations as low as one out of 1,000,000(0.0001%) total copies. What was previously undetectable with other methods can now bequantified with Droplet Digital PCR.
SUCCESS STORY
Detecting T790M mutation in epidermal growth factor receptor (EGFR), an importanttherapeutic target in some lung cancers, allows for a better understanding of resistanceto tyrosine kinase inhibitor therapies. However, other techniques lacked the sensitivity toreliably identify the T790M allele in a high background of EGFR wild type. Using Droplet
Digital PCR, a researcher in the biotechnology industry was able to develop a highly
accurate assay to quantify T790M concentration.

G T
A Mutation
2. Cancer/DNA
G T
C C
G
C C Laboratories, Inc.
Bio-RadCancer Biomarkers

DETECTION OF BRAF V600E MUTATION
11000
10000
9000
8000
7000
6000
5000
4000
3000
2000
1000
0
0

B

FAM amplitude

2000

4000

		

8000

6000

8000

11000
10000
9000
8000
7000
6000
5000
4000
3000
2000
1000
0
0

2000

4000

		

C

HEX amplitude

2000
Concentration, copies/µl

6000

HEX amplitude

1500

1880
1640

1590

1920

6
1850

1680

5
3.76

3.77

3.78

4
3

1000

2
500
1
0

73.6

0

0

0

100%
wild type100%
wild type100%
wild type

Mutant75.3
Mutant72.4

Fractional abundance, %FAM amplitude

A0

MutantSampleHigh sensitivity of the QX200 System allows quantificationof BRAF V600E mutation in the presence of wild-type DNA
using PrimePCR ddPCR Assays. A, 2-D fluorescence amplitude
plot generated by QuantaSoft Software shows triplicate wells of amixed mutant and wild-type sample. The black cluster on the plot
represents the negative droplets, the green cluster represents thedroplets that are positive for wild-type DNA only, the blue cluster
represents the droplets that are positive for mutant DNA only, andthe orange cluster represents the droplets that are positive for bothmutant and wild-type DNA. B, 2-D fluorescence amplitude plotshows three replicates of a wild-type–only sample. C, fractional
abundance plot shows the percentage frequency (orange markers)of the mutant DNA in a wild-type DNA background. The blue
markers indicate the concentration of mutant DNA (copies/µl)
and the green markers indicate the concentration of wild-type
DNA (copies/µl) in each of three replicate samples. All error
bars are generated by QuantaSoft Software and represent a
95% confidence interval.

Visit bio-rad.com/QX200 for more information.

WHAT’S IN A DROPLET?

INSIGHT INTO

VIRAL
RESERVOIRSPrecisely quantifying viral load is crucial for characterizing disease states and developing
and validating therapies. Viral reservoirs fluctuate, sometimes dropping to very low levels thatare nonetheless significant. Obtaining exact and reproducible measurements of viral DNA
or RNA often represents the difference between success and failure in studying manydifferent pathogens.
The QX200 System has the sensitivity to register extremely minute quantities of viral genetic
material and distinguish it from complex mixtures. Investigators can obtain actual copy
numbers of viral genome by generating complementary DNA through reverse transcription.
Along with precision and reliability, the QX200 System also offers users the throughput needed
to process samples with great efficiency.
SUCCESS STORY
A researcher compared quantitative PCR (qPCR) and Droplet Digital PCR approaches
for detecting residual human immunodeficiency virus (HIV) infection in a clinical sample
set, which included an infant who was functionally cured of HIV. The researcher foundthat Droplet Digital PCR was five times more sensitive than qPCR when measuring
HIV DNA copies/million cells, and more than 20 times more accurate when measuring
viral long terminal repeats (2-LTR circles).

Bio-Rad Laboratories, Inc.

Viral Load

DETECTION OF HERPES SIMPLEX VIRUS AND b2 MICROGLOBULIN
A
12000

FAM amplitude

10000
8000
6000
4000
2000
0
0

2000

4000

6000

8000

10000

HEX amplitude

B

2500

2000

1960

1500
1000

1500
1030

1030

1040

500
0

2000

1030

975

1030 1030

500

484
125

0
0

2500

1000

HEX concentration,
copies/µl

FAM concentration,
copies/µl

2500

240
0
5000		10000

20000

40000

HSV-1, copies/wellDroplet Digital PCR enables precise and reproducible
detection of herpes simplex virus 1 (HSV-1) and ß2
microglobulin (B2M) targets in a duplex assay. A, 2-D
fluorescence amplitude plot shows three merged replicate samplesof 10,000 copies/well of HSV-1 duplexed with 20,000 copies/well of
B2M. The black cluster on the plot represents the negative droplets,
the green cluster represents the droplets that are positive for HSV-1
only, the blue cluster represents the droplets that are positive for
B2M only, and the orange cluster represents the droplets that are
positive for both HSV-1 and B2M targets. B, concentration plotshows merged triplicate wells across an HSV-1 sample dilution
series from 0 to 40,000 copies in a constant background of
20,000 copies (66 ng human genomic DNA) of B2M. The blue
markers indicate HSV-1 copies/µl and the green markers indicate
B2M copies/µl. All error bars are generated by QuantaSoft Softwareand represent a 95% confidence interval.

Visit bio-rad.com/QX200 for more information.

WHAT’S IN A DROPLET?

DISTINGUISHINGGENOMIC
VARIATIONS

Copy number variation (CNV) is a prominent source of interindividual variability in the humangenome. CNV has also been associated with cancers, neurological and autoimmune diseases,
and adverse drug responses. Reliably identifying such CNVs represents an important capability
in cutting-edge research.
The major technical hurdle in copy number (CN) assessment is the ability to discriminate, withstatistical confidence, between consecutive CN states. Fundamentally, as CN state increases,
the percentage difference in target genomic material between states decreases, making it harder
to measure CNV at higher orders. By partitioning the amplification reaction across thousands ofdroplets, the QX200 System can resolve high consecutive copy number states (such as five vs. six)with an excellent degree of statistical confidence.
SUCCESS STORY
A researcher investigating a highly duplicated gene involved in human brain size evolution neededa method to validate small CN variations in test samples. Droplet Digital PCR proved much more
effective than other methods the researcher had previously used, showing greater precisionin detecting small differences at high CN states, as well as excellent reproducibility. These
improvements in CNV quantification will allow the researcher to ask more complex questions
regarding neurogenetic disorders such as micro/macrocephaly, schizophrenia, and autism.

Bio-Rad Laboratories, Inc.

Genomic Variation

DETECTION OF COPY NUMBER FOR
MYELOCYTOMATOSIS ONCOGENE AND b-GLOBIN
A
12000

FAM amplitude

10000
8000
6000
4000
2000
0
0

1000

2000

3000

4000

B
14

13.4

12
Copy number

5000

HEX amplitude

11.1

10

12.8

11.1

8
6
4.8

4
2
1.04

0
NTC

2.13
1.04

3.03
2.19

5.95
4.93

6.08

3.08

NTC CN1 CN1 CN2 CN2 CN3 CN3 CN5 CN5 CN6 CN6 CN11 CN11 CN13 CN13
SampleThe QX200 System provides superior resolution with copy
number (CN) calls from CN1 to CN13. A, 2-D fluorescence
amplitude plot shows duplicate wells of a CN6 sample duplexedwith myelocytomatosis oncogene (MYC) and b-globin (HBB) assays.
The black cluster on the plot represents the negative droplets, thegreen cluster represents the droplets that are positive for MYC only,
the blue cluster represents the droplets that are positive for HBB
only, and the orange cluster represents the droplets that are positive
for both MYC and HBB targets. B, copy number plot shows samples
ranging from CN1 to CN13 with precise duplicate well values.
All error bars are generated by QuantaSoft Software and represent
a 95% confidence interval. NTC, no template control.

Visit bio-rad.com/QX200 for more information.

WHAT’S IN A DROPLET?

NGSVALIDATION ANDCLIBRARY

T G
C A
G T
C
G
C A
G T
C A
G
G T
C A
G
G T
C A
SUCCESSG STORY G

Bio-Rad’s ddPCR platform can be easily incorporated into the nextgeneration sequencing (NGS) library preparation workflow to precisely
quantify and balance sequencing libraries on sequencers. In addition toprecise quantification, the data plots generated by the QX200 System
are rich with qualitative library information, which is not available withother currently available methodologies. Digital PCR enables consistentlibrary loading and efficient utilization of next-generation sequencers.
After the NGS run, Droplet Digital PCR can also be used to validate theresults obtained by NGS, including genomic alterations such as single
nucleotide polymorphisms, mutations, and copy number variations.

STUDIESCG

A research group used Droplet
Digital PCR to accurately quantify
five NGS libraries of varying
concentration. Bio-Rad’s ddPCR
platform allowed the researchers
to achieve an optimal cluster
density of approximately
800,000/mm2 required forsequencing on the Illumina
MiSeq platform. Tests for librarybalancing showed that libraries
could be balanced to within lessthan 15% of each other with aconfidence interval of 95%.

T

C A Mutation
2. Cancer/DNA
G

T G
C A
G T
G
C A
G T
C A
G
G T
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G
G T
C A
G
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C

C

With its high sensitivity, the QX200 System is especially well-suitedfor discerning and quantifying rare transcripts, offering a better
understanding of actual RNA function. With its high precision, theQX200 System can be used to detect small fold changes in expressionof a target gene between samples, providing insights into the gene’s
physiological implications. The ability to measure absolute values of
RNA molecules adds a new level of insight to gene expression studies.
The QX200 ddPCR System allows for such measurements without
requiring a standard curve.

Bio-Rad Laboratories, Inc.

TC

QUANTIFICATIONGENE
EXPRESSIONGC A Mutation
2. Cancer/DNA
G

SUCCESS STORY
A researcher used Droplet Digital
PCR to study the differential
expression of huntingtin (HTT)mRNA encoded by both wild-type
and mutant HTT genes. Quantifying
these different transcripts of
RNA gave the researcher moreinsight into their cellular functions,and laid the groundwork forbetter understanding diseasemechanisms.

A
14
8,192 copies/µl =163,840 copies/well
122 bp GAPDH(experiment 1)
122 bp GAPDH(experiment 2)
B2M (experiment 1)
B2M (experiment 2)
SDHA (experiment 1)
SDHA (experiment 2)
TBP (experiment 1)
TBP (experiment 2)
0.25 copies/µl =5 copies/welllog2 average copies/µl

12
10
8
6
4
2
0
–2
–4
–10

–5

0

5

10

log2 pg of RNA equivalent input cDNA

B
Gene

Droplet Digital PCR, copies/well

GAPDH

100 ng RNA
4 replicates

1,000 ng
4 replicates

MiSeq, RPKM
100 ng2 replicates

1,000 ng
2 replicates

1,671 ± 115*

16,275 ± 479*

974

953

1,077

1,061

B2M

504 ± 46*

3,450 ± 155*

233

251

229

237

SDHA

139 ± 14*

1,131 ± 81*

51

50

59

65

HPRT1

15,781 ± 2,310

140,705 ± 11,059

34

22

25

25

TBP

3,650 ± 178

31,625 ± 1,010

3

3

6

3

GUSB

1,794 ± 53

15,731 ± 1,134

9

12

13

12

Droplet Digital PCR provides nearly 1,000-foldgreater sensitivity and an amplification bias–
free approach compared to RNA-Seq for
validation. A, concentration plot shows a twofold
dilution of cDNA generated using Bio-Rad’s iScript™
Advanced cDNA Synthesis Kit for RT-qPCR. Two
independent measurement sets were made: one
at a high concentration range and one at a lower
range, with four points overlapping. B, table shows
comparative results between Droplet Digital
PCR and MiSeq. Droplet Digital PCR detected
thousands of copies per well with low-abundance
transcripts (TBP and GUSB genes) using an
input of 100 ng total RNA, which demonstrates
enhanced sensitivity. The MiSeq Sequencer
detected only single-digit reads per kilobase per
million reads (RPKM) for these transcripts.

* Obtained with cDNA diluted 200-fold because of its high abundance.

GENE EXPRESSION ANALYSIS OF STEM CELL MARKERS

10 –1
cKIT/PPIA
CD133/PPIAOCT3/OCT4/PPIAECAD/PPIA

10 –3
10 –4
10 –5

S10 (TGF β +)S9 (TGF β –)S8 (TGF β +)S7 (TGF β –)S6 (TGF β +)S5 (TGF β –)S4 (TGF β +)S3 (TGF β –)10 –7

S2 (TGF β +)10 –6

S1 (TGF β –)Normalized, copies/µl

10 –2High sensitivity and precision of the QX200
System allow reliable detection of small foldchanges with rare transcripts. The TGF β
signaling pathway plays a role in ovarian stem cell
regulation, and the addition of TGFβ will increase
the number of stem cells in the population,
increase the expression of putative stem cellmarkers, and decrease terminal differentiation
markers like E-cadherin (ECAD). Data show
expression levels of cKIT, CD133, OCT3/OCT4,
and ECAD genes across ten samples with andwithout TGFβ treatment, normalized to PPIAin this study. Reproducible detection of cDNA
copies as low as 0.5–1 target/µl in a 20 µl reaction
was achieved. S, sample.

Visit bio-rad.com/QX200 for more information.

Next-Generation Sequencing and Gene Expression

VALIDATION FOR NEXT-GENERATION SEQUENCING

WHAT’S IN A DROPLET?

ABSOLUTEQUANTIFICATIONWITH EvaGreen

C

G

T

C A Mutation
2. Cancer/DNA
G

T G
C A
G T
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C A
G T
C A
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G T
C A
G
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C A
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C

C

Through droplet partitioning and statistical analysis, Droplet Digital PCR
provides absolute quantification without the need for a standard curve,
bringing substantial improvements to many applications that requiredetection of low-abundance target copies or distinction of small foldchanges between samples. Combining Droplet Digital PCR with the powerof EvaGreen detection chemistry enables genotyping and gene expression
analysis for low-level target genes. The QX200 ddPCR System enables
absolute quantification using EvaGreen chemistry, making suchassays ideal for target DNA measurement, viral load analysis, andmicrobial quantification.

VALIDATED

ddPCR

SUCCESS STORY
Precision cancer medicine involves
rapid assessment of cancer
mutations, with certain genetic
errors serving as indicators for a
specific therapy. A researcher waschallenged with the compositionand degradation of clinical samples
that were used for assessment of
FLT3 mutations for gastric tumors(sporadic diffuse). By partitioning
the samples’ DNA templates,
Droplet Digital
G PCR
T removed the
C C
A PCR
2. Cancer/DNA
Mutation
G inhibitors
effects ofand bias inT
C C
GA resulting
the
data,
in
a
more
reliable
G
C
T G
C A
quantification
the mutations
G of
T
C A
G
present
inGthe samples.
T
C A
G
G T
C A
G
G

ASSAYS

Bio-Rad’s PrimePCR ddPCR* Assays are fully wet-lab validated assays,
expertly designed to provide single-copy PCR resolution without the useof a standard curve. These mutation detection and copy number assays
have been validated specifically for digital PCR.
Bio-Rad’s PrimePCR Real-Time PCR Assays* can also be used on
the QX200 Droplet Digital PCR System with QX200 ddPCR EvaGreen
Supermix. Choose from more than 40,000 existing human and mousegene expression assays.
* Each assay for the human, mouse, and rat genomes was experimentally tested for optimal efficiency, specificity,
sensitivity, and linear dynamic range.

Bio-Rad Laboratories, Inc.

SUCCESS STORY
Designing an optimal probe assay
may pose challenges and delay
generation of reliable data. A
researcher spent a great deal oftime trying to design probes andprimers for EGFR L858R, a receptor
for epidermal growth factor, whichis associated with lung tumors.
Using the fully validated PrimePCR
ddPCR Mutation Detection Probe
Assay for EGFR L858R eliminatedthese tedious optimization steps,
allowing the researcher to focus
on the biology of the investigation,rather than assay design.

A

B

104
103
102
10

IL-1, copies/well
NTC

25000
2810
2880
2840
1200
1210
1200
599
606
613
269
270
271
134
133
131
63.8
63.9
63.8
31.7
34.8
33.3
16.8
15.6
16.2
8.9
7.17
8.01
3.99
3.83
3.68

0

0.662

10 –1

NTC
100
200
400
780
1560
3120
6260
12500
25000
50000
NTC
100
200
400
780
1560
3120
6260
12500
25000
50000
NTC
100
200
400
780
1560
3120
6260
12500
25000
50000

10 –2

100

200

400

780

1560

3120

6260

12500

25000

20000

FAM amplitude

Concentration, copies/µl

105

15000

10000

IL-1, copies/well
5000

0
0

50000

100000150000

Event number

Droplet Digital PCR enables precise and reproducible detection calls across a range of sample concentrations. A, concentration plot shows
data for both merged wells (solid blue markers) and individual replicate wells (open blue markers) across a dilution series. Interleukin 1 (IL-1) samples
ranging from 0 to 50,000 copies were detected with an EvaGreen Assay and plotted on a log scale. There is excellent linearity and reproducibility
between replicates. B, 1-D fluorescence amplitude plot shows a sample dilution curve detected with an EvaGreen IL-1 Assay in the FAM/EvaGreen) and negative () data points. All error bars arechannel of the QX200 Droplet Reader. The data demonstrate good separation between positive (generated by QuantaSoft Software and represent a 95% confidence interval. NTC, no template control.

DETECTION OF EGFR L858R MUTATIONA

FAM amplitude

8000
6000
4000
2000
0
0

2000

4000

6000

8000

10000

HEX amplitude

B
6
1820

1730

1800

1500

1770

1790

4.12

4.21

1780

3.94

1000

5
4
3
2

500

1
0

0

0

100%
wild type100%
wild type

76

0

100%
wild type

Mutant78.5

Mutant73

Fractional abundance, %Concentration, copies/µl

2000

PrimePCR ddPCR Assays are fully validated forthe detection of EGFR L858 mutation. A, 2-D
fluorescence amplitude plot shows triplicate wells of amixed mutant and wild-type sample. The black clusteron the plot represents the negative droplets, the green
cluster represents the droplets that are positive forwild-type DNA only, the blue cluster represents thedroplets that are positive for mutant DNA only,
and the orange cluster represents the droplets thatare positive for both mutant and wild-type DNA.
B, fractional abundance plot shows the percentage
frequency (orange markers) of the mutant DNA in awild-type DNA background. The blue markers indicate
the concentration of mutant DNA (copies/µl) and thegreen markers indicate the concentration of wild-type
DNA (copies/µl) in each of three replicate samples.
All error bars are generated by QuantaSoft Softwareand represent a 95% confidence interval.

0

MutantSampleVisit bio-rad.com/QX200 for more information.

50000

Absolute Quantification and PrimePCR™ ddPCR Assays

ABSOLUTE QUANTIFICATION USING EvaGreenMore Droplet Digital PCR Resources
Visit bio-rad.com/web/QX200 for a wide range of videos, technical reports, published findings, and otherresources describing Bio-Rad’s unique Droplet Digital PCR technology and the QX200 ddPCR System.
Specifications

Catalog #QX200 Droplet Generator
Starting sample size
Capacity
Droplets per sample
Dimensions (W x D x H)

20 µl
1–8 samples/cartridge
20,00028 x 36 x 13 cm (11 x 14 x 5 in.)

QX200 Droplet Reader
Precision
Linear dynamic range
Capacity
Droplets per 96-well plate
Sample illumination
Sample detection
Detection channels
Dimensions (W x D x H)

±10%
5 orders of magnitude
1–96 samples
Approximately 1,500,000
Light-emitting diodes
Multipixel photon counter
FAM (EvaGreen), HEX (VIC)
66 x 52 x 29 cm (26 x 20 x 11 in.)1863021	
One-Step RT-ddPCR Kit for Probes, 2 ml (2 x 1 ml),
200 x 20 µl reactions, 2x RT-ddPCR mix, includes
1 manganese acetate tube
1863022	
One-Step RT-ddPCR Kit for Probes, 5 ml (5 x 1 ml),
500 x 20 µl reactions, 2x RT-ddPCR mix, includes
2 manganese acetate tubes
1864033	Q X200 ddPCR EvaGreen Supermix, 2 ml (2 x 1 ml),
200 x 20 µl reactions, 2x supermix
1864034	
Q X200 ddPCR EvaGreen Supermix, 5 ml (5 x 1 ml),
500 x 20 µl reactions, 2x supermix
1864035	Q X200 ddPCR EvaGreen Supermix, 25 ml (5 x 5 ml),
2,500 x 20 µl reactions, 2x supermix
1864036
Q X200 ddPCR EvaGreen Supermix, 50 ml (10 x 5 ml),
5,000 x 20 µl reactions, 2x supermix
1863052
ddPCR Buffer Control Kit, 9 ml (2 x 4.5 ml), 2x buffer
1864052	Q X200 Buffer Control for EvaGreen, 9 ml (2 x 4.5 ml), 2x buffer
1863040	
ddPCR Library Quantification Kit for Illumina TruSeq,
200 x 20 µl reactions, includes ddPCR Supermix for Probes(No dUTP) (2 x 1 ml vials), ddPCR Library Quantification Assay(1 x 200 µl vial), for quantification of Illumina TruSeq libraries
using the QX200 or QX100 System
1863041	
ddPCR Library Quantification Kit for Ion Torrent,
200 x 20 µl reactions, includes ddPCR Supermix for Probes(No dUTP) (2 x 1 ml vials), ddPCR Library Quantification Assay(1 x 200 µl vial), for quantification of Ion Torrent libraries using
the QX200 or QX100 System
Thermal Cycler and Plate Sealer
1851197	C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction
Module, includes C1000 Touch Thermal Cycler Chassis, 96–deepwell reaction module, USB flash drive
1814000	
PX1™ PCR Plate Sealer, includes heat sealing instrument, plate
support block that holds 96-well and 384-well plates, sealing frame,
power cord

Ordering Information
Catalog #Description

QX200 Droplet Digital PCR System
1864001	
Q X200 Droplet Digital PCR System, includes droplet generator,
droplet reader, laptop computer, software, associated component
consumables
1864002	
Q X200 Droplet Generator, includes droplet generator,
1 pkg of 24 DG8 Cartridges, 1 pkg of 24 DG8 Gaskets,
2 cartridge holders, 1 power cord
1864003	
Q X200 Droplet Reader, includes droplet reader, ddPCR manual,
2 plate holders, USB cable, power cord
1864007	Droplet Generator Cartridges and Gaskets, includes 5 pkg
of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets
1864008	
DG8 Cartridges for QX200/QX100™ Droplet Generator,
1 pkg of 24 cartridges
1863009	
DG8 Gaskets for QX200/QX100 Droplet Generator,
1 pkg of 24 gaskets
1863051
DG8 Cartridge Holder
1863005
Droplet Generation Oil for Probes, 10 x 7 ml
1864005
QX200 Droplet Generation Oil for EvaGreen, 2 x 7 ml
1864006
QX200 Droplet Generation Oil for EvaGreen, 10 x 7 ml
1863004
ddPCR Droplet Reader Oil, 2 x 1 L
ddPCR Reagents
1863026
ddPCR Supermix for Probes, 2 ml (2 x 1 ml), 200 x 20 µl reactions,
2x supermix
1863010	ddPCR Supermix for Probes, 5 ml (5 x 1 ml), 500 x 20 µl reactions,
2x supermix
1863027 	ddPCR Supermix for Probes, 25 ml (5 x 5 ml),
2,500 x 20 µl reactions, 2x supermix
1863028	ddPCR Supermix for Probes, 50 ml (10 x 5 ml),
5,000 x 20 µl reactions, 2x supermix
1863023	
ddPCR Supermix for Probes (No dUTP), 2 ml (2 x 1 ml),
200 x 20 µl reactions, 2x supermix
1863024	
ddPCR Supermix for Probes (No dUTP), 5 ml (5 x 1 ml),
500 x 20 µl reactions, 2x supermix
1863025 	ddPCR Supermix for Probes (No dUTP), 25 ml (5 x 5 ml),
2,500 x 20 µl reactions, 2x supermix

Description

EvaGreen is a trademark of Biotium, Inc. Bio-Rad Laboratories, Inc. is licensed
by Biotium, Inc. to sell reagents containing EvaGreen Dye for use in real-time PCR,
for research purposes only. FAM, HEX, Ion Torrent, and VIC are trademarks of Life
Technologies Corporation. Illumina, MiSeq, and TruSeq are trademarks of Illumina, Inc.
Illumina is not affiliated with Bio-Rad Laboratories, Inc.
The QX100 or QX200 Droplet Digital PCR System and/or its use is covered by claimsof U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by orunder license to Bio-Rad Laboratories, Inc. Purchase of the product includes a limited,
non-transferable right under such intellectual property for use of the product for internal
research purposes only. No rights are granted for diagnostic uses. No rights are
granted for use of the product for commercial applications of any kind, including but
not limited to manufacturing, quality control, or commercial services, such as contract
services or fee for services. Information concerning a license for such uses can beobtained from Bio-Rad Laboratories. It is the responsibility of the purchaser/end user
to acquire any additional intellectual property rights that may be required.
Bio-Rad’s thermal cyclers and real-time thermal cyclers are covered by one or moreof the following U.S. patents or their foreign counterparts owned by Eppendorf AG:
U.S. Patent Numbers 6,767,512 and 7,074,367.
The QX200 Droplet Digital PCR System and Automated Droplet Generator arefor research use only and are not intended for use in diagnostic procedures.

Bio-RadLaboratories, Inc.
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Life ScienceGroup

Bulletin 6311 Rev C

US/EG

15-08460615

Sig 1214