Chromatography Resins

Native and Recombinant Protein Purification
Resins/Media Selection Card
Bio-Rad offers a wide range of resins/media for both recombinant and native protein purification at scales ranging
from micrograms to kilograms.
Use this card to select the optimal resin/media for your protein purification.

Visit bio-rad.com/PPResins for more information.

Optimal Resins/Media for Native and Recombinant Protein Purification
Property

Process Purification Resin Type

Resin typeNuvia™ Q

Nuvia S

UNOsphere™ Q

UNOsphere S

Nuvia IMAC

CHT™ Ceramic
Hydroxyapatite

Nuvia™
cPrime™

Chromatography typeAnion
exchange

Cationexchange

Anionexchange

Cationexchange

Affinity

Mixed-mode(cation exchange andmetal affinity)

Mixed-mode(HIC and cationexchange)

Particle size

85 ± 15 µm

85 ± 15 µm

120 µm80 µm

38–53 µm

20 ± 2, 40 ± 4,
80 ± 8 µm

70 ± 10 µm

Ionic capacity

100–170 µeq/ml

90–150 µeq/ml

120 µeq/ml

269 ± 50 µeq/ml

N/A—

110–150 µeq/ml

Dynamic binding
capacity (DBC)≥170 mg/ml at
300 cm/hr*≥110 mg/ml at
300 cm/hr*≥180 mg BSA/ml at
150 cm/hr*

60 mg IgG/ml at150 cm/hr*≥40 mg/ml at
300 cm/hr*≥25 mg lysozyme/g*>40 mg hIgG/ml(at 10% breakthrough)at 300 cm/hr*≥125 mg BSA/ml at
600 cm/hr*

25–60 mg IgG/ml at
300 cm/hr*>60 mg lactoferrin/ml*

Recommended linear
flow rate

50–600 cm/hr

50–300 cm/hr

50–300 cm/hr

50–300 cm/hr

50–300 cm/hr

50–300 cm/hr

50–600 cm/hr

pH stability

Short-term: 2–14

Short-term: 2–14

1–14

1–14

2–14

6.5–14

Short-term: 3–14

Long-term: 4–12

Long-term: 4–13

Resin type

Macro-Prep® Methyl

Macro-Prep t-Butyl

Macro-Prep High Q

Macro-Prep High S

Macro-Prep CM

Macro-Prep DEAE

Chromatography type

HIC

HIC

Anion exchange

Cation exchange

Cation exchange

Anionexchange

Particle size

50 µm

50 µm

50 µm

50 µm

50 µm

50 µm

Ionic capacity<2 µeq/ml

120 µeq/ml

400 ± 75 µeq/ml

160 ± 40 µeq/ml

210 ± 40 µeq/ml

175 ± 75 µeq/ml

Dynamic bindingcapacity (DBC)

15 mg HSA/ml*

25 mg HSA/ml*≥37 mg BSA/ml*>49 mg hIgG/ml*≥25 mg hemoglobin/ml*≥30 mg BSA/ml*

Recommended linear
flow rate

100–600 cm/hr

100–600 cm/hr

50–300 cm/hr

50–300 cm/hr

50–300 cm/hr

50–300 cm/hr

pH stability

1–10

1–10

1–10**

1–10**

1–12

1–10

Long-term: 4–13

Property

Process Purification Resin Type

BSA bovine serum albumin; HIC, hydrophobic interaction chromatography; hIgG, human IgG; HSA, human serum albumin.
* Go to the product detail page or bulletin 6713 on bio-rad.com to see how the DBC was determined and for other technical details.
** The use of basic reagents greater than pH 10 should be evaluated for each application.

Ordering Information
Nuvia Q

Catalog #SizeNuvia S

Catalog #Size

UNOsphere Q

Catalog #SizeForesight™ Nuvia Q Plates

732-4703

Foresight Nuvia S Plates

732-4701

732-4804
732-4805
732-4721
732-4741
15604111560413156-0415
156-0417

Foresight Nuvia S
RoboColumn Unit
Foresight Nuvia S Column732-4801
732-4802732-4720
732-4740
15603111560313156-0315
156-0317

2 x 96-well,
20 µl
200 µl600 µl1 x 1 ml1 x 5 ml
25 ml
100 ml500 ml10 L

Foresight UNOsphere QPlates
Foresight UNOsphere Q
RoboColumn Unit
Foresight UNOsphere QColumnUNOsphere Q Resin732-4714

Foresight Nuvia Q
RoboColumn Unit
Foresight Nuvia Q Column

2 x 96-well,
20 µl
200 µl600 µl1 x 1 ml1 x 5 ml
25 ml
100 ml500 ml
10 L

732-4819
732-4820
732-4732
732-4752
1560101
1560103156-0105156-0107

2 x 96-well,
20 µl
200 µl600 µl
1 ml
5 ml
25 ml
100 ml500 ml10 L

UNOsphere S

Catalog #Size

Nuvia IMAC

Catalog #Size

Nuvia cPrime

Catalog #SizeForesight UNOsphere SPlates
Foresight UNOsphere S
RoboColumn Unit
Foresight UNOsphere S
Column
UNOsphere S Resin732-4710

Bio-Scale™ Mini Nuvia IMAC
Cartridges

78008111 x 5 ml

78008125 x 5 ml

Nuvia IMAC Resin

7800800

25 ml

7800801

100 ml

780-0802

500 ml12003233

5L

Foresight Nuvia cPrime
PlatesForesight Nuvia cPrime
RoboColumn Unit
Foresight Nuvia cPrime
ColumnNuvia cPrime Resin732-4705

732-4813
732-4814
732-4730
732-4750
15601111560113156-0115
156-0117

2 x 96-well,
20 µl
200 µl600 µl
1 ml
5 ml
25 ml
100 ml500 ml
10 L

12002782

10 L

732-4807
732-4808
732-4722
732-47421563401
1563402156-3403
156-3404
156-3405
156-3406

2 x 96-well,
20 µl
200 µl600 µl
1 ml
5 ml
25 ml
100 ml500 ml
1L
5L
10 L

Macro-Prep Methyl

Catalog #Size

Macro-Prep t-Butyl

Catalog #Size

Macro-Prep CM

Catalog #Size

Macro-Prep Methyl
HIC Resin

158008025 ml

1580090

25 ml

Macro-Prep CM Resin

1580070

25 ml

1560080

100 mlMacro-Prep t-Butyl
HIC Resin

1560090

100 ml1560070

100 ml

156-0081

500 ml

156-0091

500 ml

156-0071

500 ml

156-0082

5L

156-0093

10 L

156-0073

10 L

156-0083

10 L

Nuvia Q Resin

Nuvia S ResinOrdering Information
CHT Ceramic
Hydroxyapatite

Catalog #Size

Macro-Prep High Q

Catalog #Size

Macro-Prep High S

Catalog #SizeForesight CHT Type I Plates

732-4716

40 µm,
2 x 96-well,
20 µl
40 µm,
2 x 96-well,
20 µl
40 µm, 200 µl
40 µm, 600 µl
40 µm, 200 µl
40 µm, 600 µl
40 µm, 1 ml
40 µm, 5 ml
40 µm, 1 ml
40 µm, 5 ml
10 g
100 g
1 kg
5 kg
10 g
100 g
1 kg
5 kg
10 g
100 g
1 kg
5 kg
10 g
100 g
1 kg
5 kg

Bio-Scale Mini Macro-Prep
High Q Cartridges

73241205 x 1 ml

7324130

5 x 1 ml

73241221 x 5 ml

Bio-Scale Mini Macro-Prep
High S Cartridges

7324132

1 x 5 ml

73241245 x 5 ml

73241345 x 5 ml

158004025 ml

1580030

25 ml

1560040

100 ml1560030

100 ml

156-0041

500 ml

156-0031

500 ml

156-0042

5L

156-00325L

156-0043

10 L

156-0033

10 L

156-0034

50 L

Foresight CHT Type II Plates

Foresight CHT Type I
RoboColumn Unit
Foresight CHT Type II
RoboColumn Unit
Foresight CHT Type I Column
Foresight CHT Type IIColumn
CHT Type I Media, 40 µm

CHT Type I Media, 80 µm

CHT Type II Media, 40 µm

CHT Type II Media, 80 µm

732-4718

732-4822732-4823
732-4825732-4826732-4735
732-4755
732-4736
732-4756
1584000
1570040
157-0041
157-0045
1588000
1570080
157-0081
157-0085
1584200
1574000
157-4100
157-4500
1588200
1578000
157-8100
157-8500

Macro-Prep High Q Resin

Macro-Prep DEAE

Catalog #Size

Bio-Scale Mini
Macro-Prep DEAE
Cartridges

73241405 x 1 ml

73241421 x 5 ml

73241445 x 5 ml

Macro-Prep DEAE Resin

158002025 ml

1560020100 ml

156-0021

500 ml

156-0022

5L156-0023

10 L

Macro-Prep High S Resin

RoboColumn is a trademark of Atoll GmbH.

Visit bio-rad.com/PPResins for more information.

Bulletin 6983 Ver A

17-07720817