CHROMATOGRAPHY

Nuvia™ HP-Q Anion Exchange Resin
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Ideal for purification oflarge biomolecules
Best-in-classbinding capacity andmechanical strength
at high flow rates

Excellentpressure
flow properties

 igh recovery andH
reproducibility
Full regulatory support

High Dynamic Binding Capacity for Efficient
Downstream Purification of Large Biomolecules
Introduction
Nuvia HP-Q is a high-performance strong anion exchange resin. It is the latest product in the Nuvia familyof high-capacity ion exchange resins and can be used for downstream purification of large molecules
such as high molecular weight (HMW) plasma proteins IgA and IgM, viruses, virus-like particles (VLPs),and PEGylated proteins. It is built on the rugged and hydrophilic UNOsphere™ epoxide base bead thatprovides the fast mass transfer kinetics and low nonspecific binding demanded by today’s process
manufacturing. The stability of this base bead and its broad chemical compatibility allows repeated useswith a long resin lifetime. Nuvia HP-Q particle size is designed to offer high dynamic binding capacity(DBC) at fast flow rates without excessive backpressure, thereby delivering excellent process economics.
Its pore size is optimized for easy accessibility and adsorption of large biomolecules, and the internal
spacer length and ligand density facilitate efficient binding of the biomolecules even at high flow rates.
Nuvia HP-Q was designed to overcome the issues faced when purifying large biomolecules with othercommercially available resins and help in downstream purification of such biomolecules at fast flow rates
without loss in DBC and recovery. IgM obtained from plasma fractionation showed DBC in the range of20–25 mg/ml with Nuvia HP-Q. Table 1 shows that higher DBC at faster flow rates was obtained withNuvia HP-Q relative to other resins from different vendors. The technical properties of Nuvia HP-Q Resinare listed in Table 2.
Table 1. Superior DBC of Nuvia HP-Q at a high flow rate relative to other commercially available resins.
Resin

Matrix Material

Particle Size, µm

Pressure, bar

Recommended
Flow Rate, cm/hr 1

DBC (IgM)

Nuvia HP-Q

UNOsphere
epoxide50

<3300

+++2

Resin 1

Dextran beads50<330+++2

Resin 2Agarose

75<3300+3Resin 3PS/DVB50<3300+2

Resin 4PMMA50<3300+2

Recommended flow rate for industrial scale column (D > 30 cm).
DBC data at 10% breakthrough.
3 Data obtained from vendor presentation.

12

Table 2. Properties of Nuvia HP-Q.

60

Property

Description

Type of ion exchanger

Strong anion

Functional group–N (CH3)3

Particle size range

38–53 μm

Total ionic capacity

48–88 µeq

Dynamic binding capacity*>50 mg/ml at 100 cm/hr

Recommended linear flow rate

50–300 cm/hr

Maximum operating pressure

3 barCompression factor~1.2Long-term pH stability

2–11Shipping solution

20% ethanol or
2% benzyl alcohol

Regeneration

1–2 M NaClSanitization

1 N NaOH

Storage conditions

20% ethanol or 0.01 N NaOH

Storage temperature

Room temperature

Chemical stability

1 N NaOH (20°C), up to 1 week

Shelf life

5 years

10% DBC of TGY, mg/ml

50+30
20100Nuvia HP-Q

Resin 1Resin 2Resin 3Fig. 2. Dynamic binding capacity (DBC) vs. residence time of Nuvia HP-Q.
Comparison of DBCs between Nuvia HP-Q and three other commercially available
resins at 1.2 and 2.4 min residence times. The resins were packed into 1 ml columns.
Thyroglobulin (TGY) solution (1.1 mg/ml) in 20 mM Tris Cl, pH 8.0 was loaded onto thecolumns until 10% breakthrough was observed. 1.2 min residence ( );2.4 min residence ( ).

Best-in-Class Binding Capacity
Nuvia HP-Q delivers on the demanding pressure flow requirementsof downstream process purification. Its optimized particle size
facilitates a high DBC at high flow rates (Figure 1). Parallel runsof thyroglobulin on Nuvia HP-Q and other commercially available
resins demonstrate that Nuvia HP-Q overcomes the compromised
productivity issue that results from low DBCs at process-scale flow
rates (Figure 2).
7060

Excellent Pressure Flow Properties
Nuvia HP-Q Resin is designed with an optimal bead size toachieve both laboratory- and process-scale purification of large
biomolecules at high flow rates without being limited by columnpressure. This leads to an increase in productivity during protein
purification. The column pressure remains below 1.5 bar at a linearvelocity of 350 cm/hr (Figure 3).
3.5
3.0Column pressure (net), bar* 10% breakthrough capacity determined with 1.1 mg/ml of thyroglobulin in 20 mM
Tris HCl, pH 8.0.

10% DBC of TGY, mg/ml

40

2.5
2.0
1.5
1.0
0.5

50

0.0
0.040

100

200

300

400

500

600

Linear velocity, cm/hr30Fig. 3. Pressure/flow performance of Nuvia HP-Q Resin. Nuvia HP-Q slurry
prepared in 1x PBS, pH 7.5 was packed into a 20 x 20 cm column by axial
compression with a compression factor of 1.2.

201000100200300Linear velocity, cm/hr

Fig. 1. Dynamic binding capacity (DBC) vs. flow velocity of Nuvia HP-Q.
The resin was packed into a 1 ml Bio-Scale™ Mini Column (0.56 x 4 cm).
Thyroglobulin (TGY) solution (1.1 mg/ml) in 20 mM Tris Cl, pH 8.0 was loaded
onto the column until 10% breakthrough was observed.

700

3010525100

2095

1590

1085

5800750102030405060
Cycle number

708090Recovery, %10% DBC, mg TYG/ml resin

Robust Performance and Recovery
Nuvia HP-Q Resin is produced by a validated manufacturing
process that ensures batch-to-batch reproducibility. The chemicalstability of Nuvia HP-Q allows the resin to perform consistently
with minimal changes to DBC or recovery even after prolonged
exposure to NaOH (Figure 4).

100

Fig. 4. Stability, reusability, and recovery with Nuvia HP-Q Resin. Thyroglobulin(TYG) solution (1.1 mg/ml) in equilibration buffer (20 mM Tris Cl, pH 8.0) was loaded
onto a 1 ml Bio-Scale Mini Column (0.56 x 4 cm) packed with Nuvia HP-Q to acompression factor of 1.2. The column was operated at 300 cm/hr. The protein
was eluted in 5 CV of elution buffer (20 mM Tris Cl, 1 M NaCl, pH 8.0) at 300 cm/hr.
Cleaning in place (CIP) was performed on the column with 3 CV of 0.5 N NaOH at
300 cm/hr followed by a 40 min hold. The 10% DBC at linear velocity of 300 cm/hr
was determined after every 10 cycles. DBC ( ); recovery ( ).

Easy Scalability from Laboratory to Bioprocess Manufacturing
Nuvia HP-Q Resin is specifically designed for easy scalability
to meet manufacturing demands. It is available in multiple
user-friendly formats, including prepacked Foresight™ Columns
and Plates for purification condition screening and bulk
bottles for pilot- to manufacturing-scale purifications. It isbacked by our regulatory support documentation and securityof supply commitment.

Technical AssistanceA regulatory support file is available upon request. Bio-Rad
Laboratories, Inc. is an ISO 9001 registered corporation. Visit
bio-rad.com/web/NuviaHPQ for details about this resin. Visit
bio-rad.com/web/ProcessResins for more information on BioRad’s complete line of process chromatography supports. For
additional information and technical assistance, contact your local
Bio-Rad office or email our process specialists at process@bio-rad.
com. In the U.S. and Canada, call 1-800-4BIORAD.
Screen this resin for your application today. Visit
bio-rad.com/web/ResinSample to request a sample.
Ordering Information
Catalog #12006693
12006691
12006660
12006659
12007023
12007022
12007018
12007019
12007033
12006994
12007020
12007021
12007013
12007014
12006908

Description
Nuvia HP-Q Media, 25 ml
Nuvia HP-Q Media, 100 ml
Nuvia HP-Q Media, 500 ml
Nuvia HP-Q Media, 5 L
Nuvia HP-Q Media, 10 L
Nuvia HP-Q Media, B.A., 25 ml
Nuvia HP-Q Media, B.A., 100 ml
Nuvia HP-Q Media, B.A., 500 ml
Nuvia HP-Q Media, B.A., 5 L
Nuvia HP-Q Media, B.A., 10 L
Foresight Nuvia HP-Q Column, 1 ml
Foresight Nuvia HP-Q Column, 5 ml
Foresight Nuvia HP-Q RoboColumn Unit, 200 µl
Foresight Nuvia HP-Q RoboColumn Unit, 600 µl
Foresight Nuvia HP-Q Plates, 20 µl

Bio-Rad
Laboratories, Inc.
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