CovariscryoPREPPRODUCT LINE®

The n on- co nt a ct , ra pi d , d ry c ryo- p u l veri zati onsys tem fo r bi o l og i ca l t i s s u eBiomolecule Extraction using Covaris Products
Tissue Disruption for Downstream Analytical Applications
Covaris dry cryo-pulverization systems have been designed to simplify tissue processing workflows. Tissues are collected in Covaris
tissueTUBEs, flash frozen in liquid nitrogen, pulverized, and then transferred to a Covaris tube for extraction powered by Adaptive
Focused Acoustics® (AFA®). The Covaris cryoPREP system is used to process tissues and extract biomolecules from a wide range ofmasses for downstream analyses including next-generation sequencing (NGS), qPCR, Microarrays, Mass Spectrometry (MS), GC-MS,NMR, and other detection methods.

LN 2
Flash-freeze in liquid nitrogen

Dry pulverize with cryoPREP

Lyse with AFA-energetics®

Flash freeze the sample in

Dry pulverize the sample to

Acoustically extract biomolecule

liquid nitrogen to thermallyincrease surface area andin an appropriate stabilizing bufferstabilize biomolecules. Samples

disrupt extracellular matrix

prior to purification or analysis.

without the need for use of

may be stored at -80 °C.

organic solvents. Samples maybe stored at -80 °C.

cryoPREP Dry Pulverizers
CP01 cryoPREP
Manual Dry Pulverizer

CP02 cryoPREP
Automated Dry Pulverizer

CP03 cryoPREP
Manual Dry Pulverizer

cryoPREP Dry Pulverizers Compatibility Table
tissueTUBE

Sample Type

TT05M

Soft Tissues

Mass Range

cryoPREP Compatibility

10 to 50 mg

CP01

CP02

30 to 300 mg

CP01

CP02

N/A

TT05M-XT

All Tissues

TT1Soft Tissues

TT1-XT

All Tissues

TT2All Tissues

300 to 2000 mg

N/A

CP02

N/A

TT3

All Tissues

2 to 10 g

N/A

N/A

CP03

2

N/A
N/A
N/A

cryoPREP CP02 Compatibility Chart
21milliTUBE 1 ml
PN 520128

milliTUBE 2 ml
PN 520132

15 x 19mm TC15 Tube
PN 520048

TT05M PPN 520141

13 x 65mm TC13 Tube
PN 520010
TT1ADPMPN 520142

16 x 100mm TC16 Tube
PN 520011

20 x 125mm TC20 Tube
PN 520012

TT1ADP13PN 520017

TT05M - 520139
TT05M XT - 520140
TT1ADP13PN 520017

TT2P
PN 520023TT1 CRYOPREP INSERTFOR THREADED ADAPTERPN 500159

TT1PPN520006
TT1 - 520001
TT1XT - 520007

TT05 INSERTION TOOLPN 500231

TT1 HOLDERPN 500095

111TT2 - 520021

TT2 HOLDERPN 500096

22NOTES:
1 INCLUDED IN TT1 HOLDER KIT - 500097

CP02 220V - 500000
CP02 110V - 500001

2 INCLUDED IN TT2 HOLDER KIT - 500140

tissueTUBEs• tissueTUBEs have an expiration date due to the manufacturing process of heat shrinking the material• Must be vented during processing in the cryoPREP

tissueTUBE Workflows
Single-site Benchtop Workflow
Remove from freezer
or rack. Move thesample into thecenter of the bag.

If bag expands, ventthe tube. Pr ocess on
the cryoPREP.
(see User Manual.)

See Resources section for User Manual links.

3

Dip in liquid
nitrogen*(see pictu re).

2

Separate Collection and Processing Sites

Add to f reezer or put
in rack for transfer.

If bag expands, ventthe tube. Pr ocess on
the cryoPREP.
(see User Manual).

Add holde r if
necessar y.

Load sample
through opening

Keep sample
away from edges
prior to impact

Dip in liquidnitrogen*(see pictu re).

If using liquid
nitrogen, dippouch only, avoid
dipping cap

tissueTUBE Recommendations with Tissue Mass and CP02 Impact Settings
TT05M/TT05M XTMass Range
Tissue Type

10 to 30 mg

TT1/TT1XT

30 to 50 mg

30 to 100 mg

TT2

100 to 300 mg

300 to 1000 mg

1000 to 2000 mg

Brain

12

1

2

46Liver232

3

46Kidney

34

3

4

66Muscle

44

4

466Heart565666

Ocular Tissue

565666

Vascular Tissue

565666Skin

666666Tumor

666666Bone666666Cartilage

6666664TT05M and TT05M-XT Product Line
Sample Mass: 10 to 50 mg
tissueTUBE

Transfer TubePlug

Insertion Tool*Holder*

TT05M (PN 520139)
TT05M-XT (PN 520140)

milliTUBE 1 ml(PN 520128)

TT05M P(PN 520141)

TT05 Insertion Tool(PN 520231)

TT1 Holder(PN 500095)

TT05M (PN 520139)
TT05M-XT (PN 520140)

milliTUBE 2 ml
(PN 520132)

TT05M P(PN 520141)

TT05 Insertion Tool(PN 520231)

TT1 Holder(PN 500095)*Insertion tool and Holder required for CP02.
tissueTUBE: TT05M and TT05M-XT (Used with CP01 and CP02 for sample mass 10 to 50 mg)
ConsumablePart #

Contents

Notes

TT05M

52013925

Soft tissues

TT05M-XT

52014025

Soft and hard tissues (e.g., heart, muscle, kidney)

TT05 Insertion tool

500231

1TT05M plug

52014125

TT1 holder

500095

1

milliTUBE 1 ml (no fiber)

520128100

milliTUBE 2 ml (no fiber)

520132100

ConsumablePart #

Contents

Notes

tissueTUBE TT05 Cold Station

5002491

Cold Station for tissueTUBE TT05

Rack 10 place tissueTUBE TT05

500284

1Sample collection rack for (10) TT05. Designed for immersion intoliquid nitrogen or dry ice bath.

cryoTUBE 2 ml520266

252 ml cryo-strorage tubes compatible with tissueTUBE TT05M whenpaired with threaded adapter (PN 520264).

tissueTUBE TT05M Insertion Tool for cryoTUBE

5006241

tissueTUBE Insertion Tool for use with the 2 ml cryogenic vial (PN520266).

TT05M cryoADAPTER

52026425

Threaded adapter for use with the 2 ml cryogenic vial and tissueTUBE
TT05M (PN 520139 and/or 520140).

TT05M and TT05M-XT

TT05M and TT05M-XT (also TT1 and TT1-XT)

TT05M and TT05M-XT

Additional Accessories

5TT1 Product Line
Sample Mass: 30 to 300 mg
tissueTUBE

tissueTUBE Adapter

Transfer Tube

Plug

Insertion Tool*

Holder*TT1 (PN 520001)
TT1XT (PN 520007)

TT1ADPM(PN 520142)

milliTUBE 1 ml
(PN 520128)

TT1P(PN 520006)

TT1 cryoPREP Insert for
threaded adapter (PN 500159)

TT1 Holder(PN 500095)

TT1 (PN 520001)
TT1XT (PN 520007)

TT1ADPM(PN 520142)

milliTUBE 2 ml
(PN 520132)

TT1P(PN 520006)

threaded adapter (PN 500159)

TT1 (PN 520001)
TT1XT (PN 520007)

TT1ADP13(PN 520017)

15x19 mm TC15 Tube
(PN 520048)

TT1P(PN 520006)

threaded adapter (PN 500159)

TT1 cryoPREP Insert forTT1 cryoPREP Insert for

TT1 Holder(PN 500095)

TT1 Holder(PN 500095)

N/A

TT1 (PN 520001)
TT1XT (PN 520007)

TT1ADP13(PN 520017)

13x65 mm TC13 Tube
(PN 520010)

TT1P(PN 520006)

N/A

TT1 (PN 520001)
TT1XT (PN 520007)

TT1 Holder(PN 500095)

N/A

16x100 mm TC16 Tube
(PN 520011)TT1P(PN 520006)*Insertion tool and Holder required for CP02.

6

TT1 Holder(PN 500095)

tissueTUBE: TT1, TT1-XT (Used with CP01 and CP02 for sample mass 30 to 300 mg)
ConsumablePart #Contents

Notes

TT1520001

25

Soft tissues (Case of 600 PN 520036)

TT1-XT

520007

25

Soft and Hard tissues (e.g., heart, muscle, kidney)(Case of 300 PN 520092)

TT1 plug

52000625

TT1, TT1-XT

TT1 Adapter milliTUBE

52014225

Recommended, for 1 and 2ml milliTUBE

TT1 Adapter 13x65 mm

52001725

For 13x65 mm (4 ml) tube 520010

TT1 Insertion Tool

5001591

Used with TT1, milliTUBE, and 13x65 adapters

TT1 Holder500095

1TT1, TT1-XT (also TT05M and TT05M-XT)

milliTUBE 1 ml (no fiber)

520128100

Used with TT1 Adapter milliTUBE and TT1 insertion tool

milliTUBE 2 ml (no fiber)

520132100

Used with TT1 Adapter milliTUBE and TT1 insertion tool

15x19mm tube

520048100

Used with TT1 Adapter 13x65 and TT1 insertion tool

13x65mm tube (with cap)

520010500

Used with TT1 Adapter 13x65

13x65mm tube and cap

520025 & 520026

125

Used with TT1 Adapter 13x65

16x100mm tube

5200191 or 520011

25 or 500

Does not need Adapter

ConsumablePart #Contents

Notes

tissueTUBE TT1 Cold Station500157

1N/A

Rack 8 place tissueTUBE TT1520142

1Holds TT1 and TT1-XT. Designed for immersion intoliquid nitrogen or dry ice bath.

TT1 & Plug/TT1-XT & Plug Case

520009/520044

300

N/A

Additional Accessories

7TT2 Product Line
Sample Mass: 300 mg to 2 g (Compatible with CP02 only)
tissueTUBE

Transfer Tube

PlugHolder

TT2(PN 520021)

20x125 mm TC20 Tube
(PN 520012)

TT2P(PN 520023)

TT2 Holder(PN 500096)

tissueTUBE: TT2 (Used with CP02 for sample mass <2g (Recommended 300 mg to 2g))
ConsumablePart #ContentsNotes

TT2520021

25Soft tissues

TT2 plug

52002325

TT2TT2 holder

500096

1TT2tissueTUBE TT2 Cold Station

500166

1

N/A

CP02 Conversion Kit TT2500091

1

Cryoprep Conversion Kit for TT2 tissueTUBEs

TT2 & Plug Case

520034300

N/A

20x125mm (TC20)

520192

25

N/A

20x125mm (TC20)

520012500

N/A

TT3 Product Line
Sample Mass: 2 to 10 g (Compatible with CP03 only)
tissueTUBEtissueTUBE: TT3 (Used with CP03 for sample mass 2 to 10g)
ConsumablePart #ContentsNotes

TT3520008

25For large tissue mass

The CP03 is designed to make smaller pieces that can then be further processed or pulverizedTT3(PN 520008)

using the CP01 or CP02. The TT3 is a bag and a plastic clip. The sample is added to the TT3 bagand the bag is folded over 1 time for the plastic clip to seal it (like a chip clip).

8Buffer Volume and Focused-ultrasonicator Accessories for the Transfer Tubes
BufferVolume

M220 Holder& Insert

S-Series
HolderME220
Rack &WaveguideE220evolution
Rack

E220
Rack

LE220
Rack

300 µlto 1.0 ml

Holder XTU(PN 500414)& Insert
milliTUBE
1 ml (PN500422)

HoldermilliTUBE
1 ml (PN500371)

Rack 4 Place
milliTUBE(PN 500520)& Waveguide4 Place(PN 500534)

Rack E220e
4 PlacemilliTUBE 1 ml(PN 500431)

Rack 24 Place
milliTUBE
1 ml (PN500368)

Rack 24 Place
milliTUBE
1 ml (PN500368)

milliTUBE
2 ml (PN520132)

1.0 to 2.0ml

Holder XTU(PN 500414)& Insert
milliTUBE
2 ml(PN500591)

HoldermilliTUBE
2 ml (PN500375)

Rack 4 Place
milliTUBE(PN 500520)& Waveguide4 Place(PN 500534)

Rack E220e
4 PlacemilliTUBE 1 ml(PN 500431)

Rack 24 Place
milliTUBE
2 ml (PN500376)

Rack 24 Place
milliTUBE
2 ml (PN500376)

15x19 mm
TC15(PN 520048)

1.0 to 1.3ml

N/A

Holder15x19 mm
Tube(PN 500156)

N/A

Rack 24
Place 15x19ml Tube (PN500154)

Rack 24
Place 15x19ml Tube (PN500154)

13x65 mm
TC13(PN 520010)

1.0 to 4.0ml

N/A

Holder
13x65 mm
Tube(PN 500011)

N/A

Rack 24 Place
13x65 mm
Tube(PN 500033)

Rack 24 place
13x65 mm
Tube(PN 500033)

N/A

Holder16x100 mm
Tube(PN 500012)

N/A

Rack 12 Place
16x100 mm
Tube(PN 500031)

Rack 12 place
16x100 mm
Tube(PN 500031)

N/A

Holder20x125 mm
Tube(PN 500051)

N/A

Rack 12 Place
20x125 mm
Tube(PN 500032)

Rack 12 Place
20x125 mm
Tube(PN 500032)

Transfer Tube
Name andPart Number

milliTUBE
1 ml (PN520128)

16x100 mm
TC16(PN 520011)

20x125 mm
TC20(PN 520012)

1.0 to 12 ml5 to 25 ml

N/AN/AN/AN/A9

Resources
cryoPREP• CP01 User Manual: https://covaris.com/wp-content/uploads/pn_010125.pdf• Tissue processing with CP01: https://covaris.com/wp-content/uploads/pn_010458.pdf• CP02 User Manual: https://covaris.com/wp-content/uploads/pn_010035.pdf• Tissue processing with CP02: https://covaris.com/wp-content/uploads/pn_010303.pdf• CP02 conversion kit: https://covaris.com/wp-content/uploads/pn_010062.pdf
tissueTUBE• TT05M: https://covaris.com/wp-content/uploads/pn_010120.pdf• TT1: https://covaris.com/wp-content/uploads/pn_010046.pdf• TT2: https://covaris.com/wp-content/uploads/pn_010057.pdf• Instructions for TT1, TT1 adapter and 13x65mm tube: https://covaris.com/wp-content/uploads/pn_010058.pdf

SupportOngoing assistance with the operation or application of the equipment and/or troubleshooting is provided via:
• Telephone			 - United States: Tel: +1 781.932.3959 during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday,				 Eastern Standard Time (EST)			 - Europe: Tel: 44 (0) 845 872 0100, during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday,				 Greenwich Mean Time (GMT-05:00)• E-mail queries to techsupport@covaris.com or applicationsupport@covaris.com

Information subject to change without notice. For research only. Not for use in diagnostic procedures.

Stay Connected!

USA: Tel: +1 781.932.3959 | Email: customerservice@covaris.com | Europe: Tel: +44 (0)845 872 0100 | Email: emeacustomerservice@covaris.com | APAC: +86 137 6427 6714 | Email: APACcustomerservice.com
Web: www.covaris.com | Applications: applicationsupport@covaris.com | Service and Support: techsupport@covaris.com | M020074_RevE_Aug2021 | 2021© Covaris, Inc.