single cell cloning

Technical Data Sheet
Version 1.5

w/ iotasciences.com/isocell
e/ info@iotasciences.com
p/ +44 (1865) 309630

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The isoCell is a compact and lightweight benchtop system designed to automate
single-cell cloning workflows and enable easy verification of monoclonality. It has low
pressure and low shear, combined with optional heating, to ensure high cell viability.

The single-cell cloning workflow of plating, feeding and harvesting cells
is automated by the integrated liquid handling system that delivers
savings on pipetting time, reagents and removes human error.

The isoCell plates single-cells into GRID chambers. These chambers do
not suffer the optical edge effects associated with conventional culture
plasticware. Therefore, single cells are visible immediately after plating
throughout the chambers which enables easy verification of
monoclonality.

This small footprint and lightweight system easily fits inside your cell
culture hood with room to spare. It is a standalone unit, with integrated
touch screen, that provides an intuitive step-by-step guide through thecloning workflow.

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Automated functionality

GRID details

Compatibility with micro titreplates

Cell dispensing accuracy andmonoclonality



GRID generation in 60 mm roundbottom dish.

Plates single-cells.

Media exchange (cell feeding).

Cell harvesting using conventional
reagents.

Workflows for adhesion and suspension
cells.

Compatible with commonly usedreagents and stem cell coatings.



One GRID contains 256 cell-culture
chambers in a single dish.



3.24 mm2 area per chamber.

Chamber volume < 1 μl.

Harvest colony into either Tissue
Culture Treated (TCT) or PCR strips.

Compatible with 96-well micro titre
plates.

Up to 94 single cell chambers per dish(out of 256)*.

Easy verification of monoclonality using
any inverted cell culture microscopewith 4x/10x objectives.
*Limited by Poisson distribution

Other system features



Nanolitre fluid handling system.

Auto-calibration and auto-levelling.



Heated bed for improved cell health.



UV light and ethanol resistant.



Rapid exchange of wetted parts.



Automated startup, shutdown andmaintenance routines.

3Software and user interfaceCapacitive touch interface (stylusincluded).

Intuitive GUI guides user through theircloning process.

Up to 36 users with nine colour codeddishes in parallel per user.

USB port for easy software updates.

PC updating (minimum) requirements:Windows 10.

Consumables

Operating requirements

Language: English.



18 colour coded dishes (TC - treatedor non-treated).



Choose from 8 strip PCR tubes or 8 TCT
flat strips (96 well plate format).



Eppendorf tubes.



FC40STAR proprietary fluid.



Humidity range: 40% to 60%Input voltage range: 100-240V AC,50/60Hz, 1.3A



Output 24V DC, 3.75A, MAX 90W

Temperature range: 16 °C – 35 °C
60 °F – 95 °F

Mechanical characteristics

Height 320 mm (12.6”)

Width 216 mm (8.5”)

Depth 241 mm (9.5”)

Mass 4.3 kg (9 lb 8 oz)

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Disclaimer
The equipment and its output are not for use in diagnostic procedures. This equipment shall
only be used in strict accordance with iotaSciences® terms and conditions.
Unless otherwise stated in the company’s terms and conditions or required at law, iotaSciences®
does not accept any liability for any loss, damage or injury resulting from the use of thisequipment. iotaSciences® disclaims all expressed or implied warranties, warranty ofmerchantability or fitness for a particular purpose. The user is responsible for determining
whether this equipment is fit for a particular purpose and suitable for the user's method ofuse or application.
This equipment may not be transferred to third parties, resold, or modified for resale.
The company reserves the right to alter, without notice the specification, design or conditionsof supply of any product or service. This publication may not be regarded as the representation
relating to the products or services concerned.
All trademarks are the property of Iota sciences Ltd and its subsidiaries unless otherwise
specified.
©iotaSciences 20205