⑰

fRD㊧The4thchina−Japan−KoreaJointSymposium

OnAnimalReproduction
Theme：

CurrentStatuSandFutureofAnimaiReprOducdonReSearCIIin
CIlina−Japan−Korea

Venue：NaniingNewCenturyHotel，Nanjing，China

Date：August18−20，2016

ProgrammrAugust18（Thursday）
RegistrationOpen

8：00−20：00

Welcomeparty
ProgramfbrAugust19（Friday）
ReglStratlOnOpen730−830

OpeningRemarks

830−920
Chair：Jian−HuiTian

SIli−En ZIlu（Chairperson，ReproductionBranch ofChineseAssociationofAnimal
ScienceandVeterinalyMedicine）

Kei−ichiroMAEDA（President，Societyfbrreproductionanddevelopmento鯖apan）

BohsukYANG（President，KoreanSocietyofAnimalReproductlOn）

LeaderofChineseAssociationofAnimalScienceandVeterinaryMedicine
LeaderofNaniingAghcurturalUniversity，China

GroupPhoto

9：20−9：45

Co餓；e／TeaBreak

9：45＿10：00

SessionlGametes，Embryos＆AnimalsinReproduction lO：00−12：00

Chair：Shao−RongGaoandKazuhiroKIKUCHICJK−1：Zeng−MingYang（SouthChinaAgriculturalUniversity，China）
EndometrialreceI）tivityanddecidualization

CJK−2：KeiMiyamoto（Universityofcambridge，UK；KindaiUniversity，Japan）The

imprOVed culture condition fbr mouSe nuelear transfer embryosとenabtesIlighlye飾cientnuclearreI，rOgrammlng

CJK−3：lnchulCI10i（ChungnamNationalUniversity，RepublicofKorea）

Tightjunctionregulationduringearlymammalianembryodevelopment
LunchBreak

12：00−13：30

SesslOn2CellslnReproductlOn

13：30−15：30

Chair：Hai−BinWangandHakhyunKa
CJK−1：KatsuhikoHayashi（KyushuUniversity，Japan）

ReconstitutionofoogenesisindishuSingmouseembryonicstemcells
CJK−2：Shao−RongGao（TongiiUniversity，China）
Epigenetic regulalioninearIyembryodeve10I）men

and somaticceli

reprOgrammlng
CJK−3：MinjungYoon（KyungpookNationalUniversity，RepublicofKorea）
The attempt tO prOduce horSeS uSing SIIermatOgOnia−

stem celltransplanlationtechniques
Session3MoIeculesinReproduction

15：45＿17：45

Chair：Zeng−MingYangandJun−YouLi
CJK−1：LeePoongyeon（AnimalBlOteChnologyDivision，NIAS，RepublicofKorea）

CurrentstatusofanimaltranSgenicresearcIIinNIASCJK−2：NahokoIeda（KyushuUniversity，Japan）
KisspeI）tinneuronSaSamaSterregulatorormammalianreproducion

CJK−3：Hai−BinWang（InstituteofZoology，ChineseAcademyorSciences，China）

Roadmaptoembryoimplantation
ClosingCeremony

17：45−18：00

Chair：Jian−HuiTian

Shi−EnZhu（ChaiIPerSOn，ReproductionBranchofChineseAssociationofAnimal
ScienceandVeterinaryMedicine）

FarewellParty18：00

CIIair：Shi−EnZhuandFengWang

ProgramrbrAugust20（Saturday）
Post−COnferenceprofessionalandsocialevents

The4ihKCJJo−n−SymposlumOnAnImalReproduct−On，Augusti8−20，Na叩ng

CONTENTSENDOMETRIALRECEPTIVITYANDDEClDUALIzATlON．…
…‥Zeng−MingYang，SouthChinaAgriculturalUnlVerSlty（1）THElMPROVED CULTURE CONDIT10N FOR MOUSE NUCLEAR TRANSFEREMBRYOSENABLESIllGIILYEFFICIENTNUCLEARREPROGRAMMING．．．…
KeiMiyamoto，UniversityorCambridge（2）TIGHTJUNCTlON REGtJLATION DURING EARLY MAMMALIAN EMBRYODEVELOPMENT・…・…………………………………‥lnchulChoi，ChungnamNationalUniversity（3）RECONSTITUTlON OFOOGENESISIN DISII USING MOUSE EMBRYONIC STEM
KatsuhlkoHayashi，KyushuUniversity（4）EPIGENETIC REGULATIONiN PRE−IMPLANTATION EMBRYO DEVELOPMENTShao−RongGao，TongjiUniversity（5）THEATTEMPTTO PRODUCE HORSES USING SPERMATOGONIALSTEM CELL（SCC）TRANSPLANTATIONTECHNIQUES
Ml巾ungYoon，KyungpookNationaiUniverslty（6）CURRENTSTATUSOFANIMALTRANSGENICRESEARCHINNIAS…．．
……LeePoongyeon，NationalInstimteofAnlmaiScience（8）KlSSPEPTIN NEURONS AS A MASTER REGULATOR OF MAMMALIANREPRODUCTION………………………………………………………Nahokoleda，NagoyaUnlVerSity（9）SIIP21SESSENTIALFORNORMALEMBRYOlMPLANTATIONiNMICE…．．
HaibinWang，ChineseAcademyofSciences（10）NEW METHOD FOR CRYOPRESERVATION OF MESENCHYMAL STEM CELLSUSINGPOINCAPROLACTONE（PCL）SCAFFOLD…‥．
・……Dong−MokLee，KorealnstituteofIndustrialTechnology，Gyeongbuk（11）LONG−TERMCRYOPRESERIIATIONOFSPERMATOGONlALSTEMCELLS…．

Yong−AnLee，Chung−AngUniverslty（12）EFFECTS OFISOPROTERENOLAND MELATONIN SUPPLEMENTAT10N ONINVITRODEVELOPMENTOFPORCINEPARTHENOGENETICOOCYTES…

……・DaUnJeong，GyeongnamNatlOnalUnlVerSityofScienceandTechnology（13）EFFECTS OF OVARYSTATUSANDTRANSFERRED CLONED EMBRYO NUMBER

ONTIIEEFFICIENCYOFPIGCLONING……・Ml−RyungPark，NatlOnalInst血teorAnimalScience，RDA，RepubllCOfKorea（14）KNOCK−INOFHUMANFGF2GENElNTHEBOVlNEBETA−CASEINGENELOCUSlmc41hKCJJolntSympos−umOnAn一ma−ReproductlOnlAugust18−20・Na叩g

USINGCRISPR／CAS9NUCLEASE一MBDIATEDHOMOLOGOUSRECOMBINATlON．．
Young−HeeJeong，ChonnamNatlOnaIUniversity（15）TAUROURSODEOXYCIlOLIC ACIDIMPROVES PRE−IMPLANTATIONDEVELOPMENTOFPORCINESCNTEMBRYOBYENDOPLASMICRETICULUMSTRESSINLIIBITION…………………………………・∴lboLln，ChungnamNationaIUniversity（16）PROGESTERONEINFLUENCESCYTOPLASMICMATURATIONIN PORCINEOOCYTESDEVELOPlNGlNVITRO………………………………．BaoYL胤LJ臨nUniverslty（17）TAUROURSODEOXYCHOLICACIDENHANCESPRE一IMPLANTATIONEMBRYOSDEVELOPMENTAFTERICSlOFEVAPORATIVEIXDRIEDBOARSPERMATOZOA…‥i・XiaoXiaLi，ChineseAcademyofagncu血ralSciences（18）CDK21S REQUIRED FOR THE DNADAMAGE RESPONSE DtJRING EARLYEMBRYONICDEVELOPMENTOFPORCINEPARTHENOTES．．…
HaiYangWang，ChungbukNationalUniverslty（20）COMPARATIVEPROTEOMICSANAL：YSlSOFHUMANANDBOVINECOLOSTRUM
MILK………………………………………………………・JaeEunLee，ChungnamNationalUnlVerSity（2DCHARACTERIZATIONOFPORCINEOCT4UPSTREAMREGULATORYREGIONSTRUCTUREANDFUNCTlON………………‥Seung−HunKjm，SeoulNationalUniverslty（22）CANHUMANlZEDMICEPREDICTDRUG●●BEHLNlOR●●INHUMANS？．
XuD，StanfbrdUniversity（23）

EXPRESSIONOFMEMBRANEPROGESTERONERECEPTORSPROGESTINAND
ADIPONECTINRECEPTORSINTHE UTERINEENDOMETRIUMDtJRINGTIlE
ESTROUSCYCLEANDPREGNANCYINPIGS……MiltieongKlm，YonselUniverslty（25）MELATONINPROMOTESFnTTYACIDSYNTHESISINPORCINEOOCYTES．．．…
Jun−XueJin，SeoulNationalUniversity（26）

OVEREXPRESSlONOFREPROGRAMMINGfACTORSONPORCINESTEMCELLSDERIVEDFROMEMBRYO……

i…‥Tae華ongPark，SeoulNationalUniversity（27）

EFFECTSOFMICROBUBBLEDWATERSUPPLYONTOTALEGGPRODUCTION
RATE，EGGWEIGHTS，ANDBODYWEIGHTINEARI」YLAYINGHENS……
・……Dong−MokLee，KoreaInstituteorlndustrialTechnoIogy（28）

PORCINELARGEFOLLICULARFLUIDIMPROVESOOCYTEMATURATIONANDEMBRYODEVELOPMENTONINVITROMATURATlONOFPIGS．…
Ji−EunPark，KangwonNatlOnalUnlVerSity（29）PORClNEPOORCOCSCOULDBERESCUEDBYCUIJUREWITHGOODCOCS．…
SoYeonKlm，ChungnamNationalUniversity（30）ll：∴∴∴lhe4甲ing：二二二二二二二二二DIETARY−lNDUCED CHRONIC HYPOTHYROIDISMNEGATIVELYAFFECTSRATFOLLICULARDEVELOPMENTANDOVULATIONRATE：lSTHEREAROLE FOROXIDATIVESTRESS？……………………………………………LiMeng，WageningenUniversIty（31）DNA DOUBLE−STRAND BREAKS DISRUPTED THE SPINDLEASSEMBLYIN
PORCINEOOCYTES…・・．…‥HaiYangWang，ChungbukNationalUnlVerSIty（32）OXIDATIVE DAMAGEIN GOAT OOCYTES DURINGIN VITRO MATURATION
ZhengrongZhou，JiangsuUniversity（33）TRANSGENESIS OFHUMANlzED FATI PROMOTES N−3POLYUNSATURATEDFATTYACID SYNTHESISAND EXPRESSION OF GENESINVOLVEDIN LIPiDMETABOLISMINGOATCELLS…………‥．YIXuanFan，NaniingAgriculturalUniverslty（34）MOUSElNBRED STRAlN OFFSPRING PRODUCED BYlNTRACYTOPLASMlC
SPERMINJECTION（ICSI）WlTHOOCYTES FROM FEMALESATAGE OF3−4WEEKS………………………………………………………‥JieZhu，theUnlVerSltyOflnnerMongolia（35）ROLESOFLNCRNAINTHE EFFECTSOFNUTRITION ON DEVELOPMENTOFTESTISDURINGSEXUALMATURATIONOFHUSHEEP．．．．．．
HuaYang，NanjingAghculturalUniverslty（36）

EFFECTOFPPARGCIAOVEREXPRESSIONORSILENCINGONMlTOCHONDRIAL
APOPTOSISOFGOATGRANULOSACELLS
Guo−MinZhang，NammgAgricultmalUniversity（37）NON−INVASIVEASSESSMENT OF CULTURE MEDIA FROM GOAT CLONEDEMBRYOSASSOCIATEDWITH SUBJECTIVE MORPHOLOGYBYGC−MSBASEDMETABOLOMICANALYSlS・…

・…‥lねn−LiZhang，Na叩ngAgricuIturalUniVerSity（38）

FUNCT10NALSNPSOFINCENPAFFECTSEMENQUALITYBYAlTERNATIVESPLIClNGMODEANDBINDINGAFFlNITYWITHTHETARGETBTA−MIR−3781N
CHINESEHOLSTEINBULLS……．JuanLiu，ShandongAcademyorAghculmralSclenCe（39）zEARALENONE EXPOSUREIMPAIRS MOUSE PRIMORDlAL FOLLICLE
FOmMATIONINVlTRO…・…………‥Guo−LlangZhang，QlngdaoAgriculturalUnlVerSity（41）EXOGENOUSROS SHORTLTERM PRETREATMENTSHEEPINVITROMATUREDCUMULUSOOCYTECOMPLEXESlMPROVESEMBRYODEVELOPMENT……
・・i…TbngZhang，InnerMongoliaAghcu血ralUniversity（43）

MULTI−TRANSGENIC PIGS SECRETlNG B−GLUCANASE，XYLANASEANDPIIYTASEINSALIVAFORIMPROVINGNUTRIENTUTILIzATIONANDEXCRElA
POLLUTlONCONTROL・i…………・Xlan−WeiZhang，SouthChlnaAgncu血I・alUniverSity（44）EFFECTS OFDIETAND L−ARGINlNETREATMENTON NOIPGC−1ASIGNALING

ilI

一m4d肥Jo血S画mm他山JR印心血nAll印一議0・N餌直唯−

PATHWDURINGTHEESTROUSCYCLEINHUSHEEP．．．…
YixuanGuo，Na申ngAgriculturalUniversity（45）METABOLOMlC PROFILINGiN UMBILICALVENOUS PLASMAREVEALSEFFECTS OF DIETARY RUMEN−PROTECTED ARGININE OR N＿CARBAMYLGLUTAMATESUPPLEMENTATIONINNtJTRIENTRESTRiCTIONHUSHEEPDURINGPREGNANCY…………‥・・LingwelSun，Na申ngAgnculturalUniversity（46）GENERATlONANDEVALUATIONOFMYOSTATINKNOCK−OUTRABEHTSANDGOATSUSINGCRISPR／CAS9SYSTEM
Rl−HongGuo，NamngAghcu血ralUniversity（48）MELATONIN SYNTHESIZED BY MITOCIlONDRlAiN MICE OOCYTE TOAMELIORATEITSFtJNCTIONANDIMPROVEOOCYTE，SQUALITY……．
JlngWang，ChinaAgnculturalUnlVerSity（49）

REPRODUCTIVEAXlSGENE REGULATIONOFPIlOTOSTIMULATIONANI）
PHOTOREFRACTORINESSOFYANGZHOUGOOSEGANDERS．…．
……・HuanxiZhu，JiangsuAcademyofAghcu血raJSciences（50）

THEEFFECTSOFDOWNREGULATINGSUV39HllIl2GENEEXPRESSlONON
DEBAOPlGFlBROBLASTCELLGROWTII…………・Xiao−LiDai，GuangxiUnlVerSity（52）CONCENTRATIONOFAMHANDMRNAEXPRESSIONOFFOURTGF−BFAMILY

GENESINRELATIONTODIFFERENTFOLLICULARDIAMETERlNBUFFALO
YapingWang，HuazhongAgricuIturalUnlVerSity（53）

OVEREXPRESSIONOFKDM4DLEADSTOLOSSOFDNAMETHYLAT10NATICRSOFXISTANDOCT41NGOATFIBROBLASTCELLS．．．…
MingtianDeng，Na埴ngAgricu血ralUnlVerSity（55）FHL2rTGF−A佃GFRFORMEDANAUTOCRINELOOPTOREGULATEKGNCELL
PROLIFERATION・・……………………………………FanLln，HuazhongAgricu血ralUniverslty（56）

TIIECHARACTERSOFBOVINEINDUCEDPLURIPOTENTSTEMCELLSDERlVED
FROMFOURYAMANAKAFACTORS……………LixlaZhao，InnerMongol−aUniVerSlty（58）

ISOZYGOUSANDSELECTABLEMARKER−FREEMSTNKNOCKOUTCLONEDPIGSGENERATEDBYTIIECOMBINEDUSEOFCRISPR／CAS9ANDCRE几OXP……
……YanzhenBi，HubeiAcademyofAgroSciences（60）THESTUDYOFI13K27ACETYLATIONINPORCINEIMMATUREOOCYTESWITH
DIFFERENTCHROMATINCONFIGURATION
ZhangLing，FuyangNomalUnlVerSity（62）DMD−MODIFIEDPIGGENERATEDBYZYGOTEINJECTiONWlTHCAS9／SGRNAIVme41hKCJJolntSymposlumOnAnlmalReproduct10m．Augus−18−20、Namng

RESUIJEDINMUSCLEDYSTROPHY…………‥HengZhao，ShanghailtchUniversity（64）

EFFECTOFBODYCONDITION ON FOLLlCLE GENE EXPRESSlONIN ESTROUS
GOATS……………………………………………………Zi−YuW厄ng，NaniingAgricu血raiUnlVerSlty（66）