miniPERM®

Bioreactor for protein production

miniPERM®
Bioreactor for protein production

Function
The miniPERM® bioreactor is a membrane-based twocompartment system.

• High cell densities• High product concentration

A dialysis membrane subdivides the culturing chamberof the miniPERM® bioreactor into a production module(optionally 35 or 50 ml cell culture volume) and nutrient
module (400 ml medium volume).

Nutrient module• Easy handling• Multiple harvesting

Production module• Range of cell culture volumes
esThe small pore size of the dialysis membrane (MWCO12.5 kD) prevents both the cells and released cell
products from passing through the membrane. Theseare accumulated to high cell densities and productconcentrations in the production module.

Cell product
GlucoseApplications
AppLactate• H
Hybridoma cells
NH4+

• Tr
Transfected cells
CO2• B
Biomass production

The outer side of the production module consists ofa thin O2- and CO2-permeable silicone membrane for
optimal gas exchange.

Cell• Vi
Virus-producing cells• E
Entomological cells

O2• PPlant cells
Silicone membrane

Dialysis membrane

The miniPERM® is an easy-to-handle bioreactor which was developed for the cultivation of eukaryotic cells(mammalian, insect and plant cells) in high density and therefore for biomass production and for the production of cellproducts.
The sub-division of the bioreactor into production and nutrient modules and the rotating cultivation allow for theproduction of highly concentrated cell products in small volumes. Therefore, depending on the cell line, cell densities ofmore than 107 cells/ml and product concentrations of several mg/ml can be achieved. This means that the miniPERM®
bioreactor is a cost-effective and time-saving alternative to using conventional roller bottles and fermentation systems.

Different cell culture volumes
Depending on the production scale, the miniPERM®
production modules are available for cultivation in two
different cell culture volumes:

miniPERM® classic “High density culture 35 ml”

Universal Turning Device
For optimal supply of nutrients and removal of metabolic
waste products during cultivation, cells are kept insuspension by continuous rotation. The Universal
Turning Device can accommodate up to 4 miniPERM®
bioreactors and is placed into a CO2 incubator at a
relative air humidity of 70% during cultivation.

Simultaneously, the metabolites released from thecells diffuse through the dialysis membrane from theproduction module into the nutrient module to be dilutedor neutralized in the medium.

The production module has a cell volume of 35 ml and isideally suited for production in laboratories or research.

miniPERM® HDC 50 “High density culture 50 ml”
The HDC 50 with a cell culture volume of 50 ml is suited forthe large-scale production of proteins and biomass.
The outer dimensions of this production module are identicalto those of the miniPERM® classic.

The bioreactor and accessories
miniPERM® bioreactors

miniPERM® accessories• miniPERM® sterile:
The production and nutrient modules are certified sterile,
pyrogen-free/endotoxin-free and non-cytotoxic and aresupplied as a single-use bioreactors.

The following accessories are available for easy handling of theminiPERM® bioreactor:• 6WHULOHVLQJOHXVHV\ULQJHV bPObPO• miniPERM® stand• miniPERM® reusable:
The nutrient module is autoclavable and designed for multipleuse. The production modules are sterile, certified pyrogenfree/endotoxin-free and non-cytotoxic and are available asindividually packaged single-use products.

• Sterile screw caps for the modules• Sterile syringe needles (25G x 5/8”)• Sterile filling tube
miniPERM® start-up support kit
This kit contains all the accessories required for starting theculture, sampling and harvesting.

Ordering information – miniPERM®
Order noDescription

Packaging units/box

94.6001.059

miniPERM® classic

Bioreactor, sterile

294.6001.055

miniPERM® classic

Production module, sterile

494.6077.121

miniPERM® HDC 50

Bioreactor, sterile

294.6077.017®

Production module, sterile

4miniPERM HDC 50

Ordering information – accessories

20_512_0200_200

This document may contain information on products that may not be available in particular countries

Technical modifications reserved

Order noDescription

Packaging units/box®

94.6001.153

Nutrient module for miniPERM , autoclavable

494.6001.054

Stands for miniPERM®

494.6001.036

Screw caps for production module, sterile

694.6077.037

Screw cap for nutrient module, sterile

1694.6077.135

Luer syringe needle, 25G x 5/8", sterile100

94.6077.136

6LQJOHXVHbPO/XHUV\ULQJHVWHULOH

10094.6077.137

6LQJOHXVHbPO/XHUORFNV\ULQJHVWHULOH

6094.6077.138

Filling tube 5", Luer, sterile50

94.6001.094

Start-up support kitQuantity

š6LQJOHXVHbPO/XHUORFNV\ULQJHVWHULOH

8

š6LQJOHXVHbPO/XHUV\ULQJHVWHULOH

20• Filling tube 5", Luer, sterile8• Luer needle, 25G x 5/8", sterile20• Septum seal, sterile6• Stand for miniPERM®

11

Ordering information – universal turning device
Order no94.6001.061

SARSTEDT AG & Co. KG
P.O. Box 12 20
D-51582 Nümbrecht
Phone +49 2293 305 - 0
Fax+49 2293 305 - 3992
export@sarstedt.com
www.sarstedt.com

Description
Universal Turning Device 115 / 230 V

Packaging units/box
1