lumox® Reference Guide
Which options do I have for the cultivation of my cells?

Come Grow with us

2Are you planning to add a new cell line to your cell culture?
Or do you want to establish a new analysis method?

No matter how different your cells and your analysis methods are – your demand for specialized
products might just be as individual. Our range of products equipped with a lumox® film baseincludes dishes, multiwell plates and slide-based x-well cell culture chambers. For easy selectionof the appropriate product, the following pages provide an overview of cells and organismsthat have already been successfully cultivated on the lumox® products as well as the intended
application or analysis performed afterwards.
This reference guide is intended to support you in selecting the ideal product for your cells andapplication. In view of the multitude of factors that have an impact on the cultivation of cells,
tissues. and organisms, we recommend to always test the products under your specific conditions.

Has your cell, organism or application not been listed yet but you have alreadytested the lumox® products? We are always interested in extending our reference guide.
Share your experience with us!

3

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lumox® products
lumox® cell culture products are characterized by their ultra-thin, gas-permeable film base. Optimum gas exchange
is guaranteed due to the gas permeability and the short diffusion paths. The lumox® film base features very low
autofluorescence and excellent light transmission, making the products highly suitable for microscopical and reader
imaging techniques. For further analyses, like e.g. electron microscopy or mass spectrometry, the lumox® film base canbe swiftly excised using a scalpel and is highly resistant against various chemicals needed.
The following products are equipped with a lumox® film bottom:
lumox® dishlumox® dish is made of a transparent polystyrene cover anda polystyrene frame with the base made of the transparent,
gas-permeable and ultra-thin (25 µm) lumox® film. lumox® dishis available with a diameter of 35 mm and 50 mm.
Additionally, the lumox® dish is available with two different
growth surfaces:
red = the hydrophilic surface provides an ideal culture
substrate for many adherent cells
green = the hydrophobic growth surface is ideallysuited for suspension cells (usually cells of lymphoidorigin, hybridoma cells etc.) which are cultured non
adherently in solution

lumox® multiwelllumox® multiwell plates consist of a black polystyrene frame witha base made of the transparent, gas-permeable and ultra-thin(50 µm) lumox® film. The external dimensions of these plates arein accordance with ANSI / SLAS standard 1-2004: Microplates –Footprint Dimensions and can be used for analyses in instruments
requiring these standard dimensions. lumox® multiwell plates areavailable in 24-well, 96-well and 384-well format with the following
surface:
red = the hydrophilic surface provides an ideal culture
substrate for many adherent cells

x-well lumox® detachable
The x-well cell culture system allows cultivation and analysis
of cells on a microscope slide. x-well lumox® detachable consistsof a polystyrene lid and frame attached to a slide. The slide itself
is comprised of the transparent, gas-permeable and ultra-thinlumox® film attached to a white frame. This frame provides stability
for handling the products and offers a writing area for labeling thesamples. x-well lumox® detachable are available in 1-well, 2-well,
4-well and 8-well formats with the following surface:
red = the hydrophilic surface provides an ideal
culture substrate for many adherent cells
For further processing, the chamber can be detached from theslide without a tool by lifting the chamber slightly. Furthermore, noresidual adhesive will be left on the slide so that further steps canbe carried out swiftly.

5Reference Guide

Cell type / tissue / organism

Description

Product

Amniotic cells

Human primary

x-well lumox®

AsPC-1

Pancreatic Cancer cell line

lumox® dishBacillus aquimarisRirmicutes bacteria

lumox® film

Bacillus subtilis

Rirmicutes bacteria

lumox® filmbEnd.3

Murine brain endothelial cell line

x-well lumox®

BT-20

Human mammary cancer cell line

lumox® multiwell

Blastocytes

Murine Blastocytes

lumox® dishBorder Cells

Drosophila egg chamber cells

lumox® dish 50 (94.6077.410)

Brain slices

Murine brain slices E12.5

lumox® dish

CaCO2

Human epithelial colon adenocarcinoma cell line

lumox® multiwell

Cardiac cells

Primary, human, cardiac origin

x-well lumox®

Chorion cells

Human primary

x-well lumox®

CM

Murine cardiale myocyten

x-well lumox®

Cortical organoids

Human PSC derived

lumox® dish

COS-7

Chinese hamster ovarien cell line

lumox® multiwell 24

Drosophila midgut

Adult, disected midgut

lumox® dish 35 (94.6077.331)

Drosophila Embryo

Whole fly embryolumox® dishDrosophila Embryo

Whole fly embryolumox® dishDrosophila Embryo

Whole fly embryolumox® film

Drosophila Embryo

Whole fly embryolumox® dishDrosophila Embryo

Whole fly embryolumox® dishDrosophila Embryo

Whole fly embryolumox® dishE.coliWild-type E. coli strain K-12

lumox® dish (94.6077.333)

E.coli BL-21 (De3)Bacteria

lumox® multiwell

Endothelial cells

Human primary, vein

x-well lumox®

ESC

Human embryonic stem cells

lumox® multiwell 24

ESC

Murine embryonic stem cell line

x-well lumox® detachable

ESC (line CCE)

Murine embryonic stem cells

lumox® multiwell

Eyelid Culture

Primary murine eyelid culture

lumox® dish

H9-Dll1 cells

Human embryonic stem cell line H9-Dll1

lumox® multiwell

HAM

Human amnion membranelumox® dish 35

HEK

Human embryonic kidney cell line

lumox® multiwell 96

HEK-293T

Human embryonic kidney cell line - Large T-Antigen

lumox® multiwell 96

HEK-293T

Human embryonic kidney cell line - Large T-Antigen

lumox® multiwell 96

HEK-293T

Human embryonic kidney cell line - Large T-Antigen

lumox® multiwell 96

HeLa

Human cervix carcinoma cell line

x-well lumox®

HeLa

Human cervix carcinoma cell line

x-well lumox®

Hemocytes

Drosophila immune cells

lumox® dishHepatocytes

Primary, human

x-well lumox®

Hepatocytes

Primary, rat

lumox® multiwell 24

Hepatocytes

Primary, rat

lumox® dish

HepG2

Human hepatocyte carcinoma cell line

lumox® dish 35

hiPSC-RPE cell sheets

Retina pigment epithelium cell sheets derived from human induced
pluripotent stem cells

lumox® dish 35

HuH7.5

Human hepatocarcinoma cell line

lumox® multiwell 24

6Analysis / application

Literature / Source

Fluorescent microscopy

Grebenstein, B. et al., Application Note

Confocal microscopy

Broughton, L. 2016, Dissertation

Biofilm culture

Lachnit, T. et al., FEMS Microbiol Ecol 84 (2013) 411-420

Biofilm culture

Lachnit, T. et al., FEMS Microbiol Ecol 84 (2013) 411-420

Fluorescent microscopy

Loserth, S., Application Note

GC-MS, LC/MS-MS

Beatty, A. et al. 2017, DOI: 10.1158/1535-7163.MCT-17-0407

Spinning disk confocal microscopy

Customer information / SARSTEDT in-house test

Live cell imaging

Customer information / SARSTEDT in-house test

Live cell imaging

Panoudopuolou, E., Green J., PLOS Biology | DOI:10.1371/journal.pbio.1002405 March 9, 2016

Fluorescent microscopy

Welk, M. et al., SARSTEDT Application Note

Bright field microscopy

Haag, M., SARSTEDT Application Note

Fluorescent microscopy

Grebenstein, B. et al., SARSTEDT Application Note

Microscopy for planimetric measurement

Straubinger, J. et al., 2017, FASEB Journal Vol. 31, No. 4, pp. 1620-1638

Cultivation

Watanabe, M. et al. 2017, doi.org/10.1016/j.celrep.2017.09.047

Fluorescent laser-scanning microscopy

Ujike, M. et al., Journal of General Virology (2016), 97, 1853–1864

Live cell imaging

He, L. et al. 2018, Nature. 2018 March 01; 555(7694): 103–106. doi:10.1038/nature25744

Microscopy

Evans I.R. et al., JoVE Journal of Visualized Experiments 2010, 36, http://www.jove.com/index/
Details.stp?ID=1696, doi: 10.3791/1696

Live imaging

Deutschman, E. et al. 2018, doi: https://doi.org/10.1534/genetics.118.301344

Live imaging

Garcia, H and Gregor, T. 2018, RNA Detection: Methods and Protocols, Methods in Molecular
Biology, vol. 1649, DOI 10.1007/978-1-4939-7213-5_23

Confocal microscopy

van den Brink DM, et al., PLoS ONE 2013, 8(5): e64427

Confocal microscopy

Zanet, J. et al., J. Cell Biol., 2012, Vol. 197 No. 4 477–486

Widefield and confocal microscopy

Matsubayashi, Y. et al., Current Biology 27, 3526–3534.e1–e4, 2017

Time-lapse fluorescence imaging

Morris, R. et al., New J. Phys. 19 (2017) 035002

Fluorescent imaging

Customer information / SARSTEDT in-house test

Fluorescent microscopy

Puschmann, C., SARSTEDT Application Note

Confocal microscopy

Bone, H. et al., Journal of Cell Science 2011, Vol. 124, 1992-200

Fluorescent microscopy

Ripoll, Y., Dissertation,"Glycogen Synthase Kinase 3 (GSK-3) involvement in regulation of mouseembryonic stem cell fate", University of Bath Department of Pharmacy and Pharmacology, 2011

Fluorescent microscopy

Customer information / SARSTEDT in-house test

Live Imaging

Heller, E. et al., Dev. Cell 28, 617–632, 2014,

Confocal Microscopy

Pallocca, G. et al., Arch Toxicol (2017) 91:3385–3402

Confocal Laser Scanning Microscopy

Customer information / SARSTEDT in-house test

Fluorescent plate reading

Customer information / SARSTEDT in-house test

Live cell imaging

Ishii, S. et al., Biosci. Biotechnol. Biochem. 2011, 76 (3), 523-529

Live cell imaging

Masuda, K. et al., PLoS ONE 2012, 7(4): e35380

Live cell imaging

Sakurai, T. et al., Biochemical and Biophysical Research Communications 402 (2010) 595–601

Fluorescent microscopy

Dehne, H.-J., SARSTEDT Application Note

Electron microscopy

Taylor, R. et al. 2018, doi: http://dx.doi.org/10.1101/401133

Live imaging

Evans et al. 2010, doi: 10.3791/1696

Bright field microscopy

Ullrich, A. et al., SARSTEDT Application Note

Cultivation

Matsui, H. et al., Biological Engineering Journal, 52/2-3, pp 255-262, 2010

Fluorescent microsocpy

Matsui, H. et al., The Royal Society of Chemistry, Lab Chip, 2012, 12, 1857-1864

GC-MS, LC/MS-MS

Ramirez, T. et al., Arch Toxicol (2018), DOI 10.1007/s00204-017-2079-6

Laser microdissection

Customer information / SARSTEDT in-house test

Electron microscopy

Jiang, B. et al. 2015, J. Virol. doi:10.1128/JVI.03109-15

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Reference Guide

Cell type / tissue / organism

Description

Product

HUVEC

Human umbilical vein endothelial cells

lumox® film

HUVEC

Human umbilical vein endothelial cells

lumox® dish 35 (94.6077.331)

HUVEC/B16F10 3D co-culture

3D co-culture of Human umbilical vein endothelial cells and B16F10 murine
melanoma cell line

lumox® dish 35 (94.6077.331)

hvEC

Human endothelial cells

x-well lumox®

IPEC-1

Intestinal porcine epithelial cells

lumox® dish

IPEC-J2

Intestinal porcine epithelial cells

lumox® dish

IPEC-J2

Intestinal porcine epithelial cells

lumox® multiwell 96

iPS

Induced pluripotent stem cells

lumox® multiwell 24

iPS

Human induced pluripotent stem cells

lumox® multiwell 24

iPS derived cortical tissue

Human induced pluripotent stem cell derived cortical tissue

lumox® dishJimt-1

Human breast cancer

lumox® multiwell 96

KRIB

v-Ki-ras transformed human osteosarcoma cells

lumox® dish 50

L929

Mouse fibroblast cell line

lumox® multiwell 96

LM8

Mouse osteosarcoma cells

lumox® dish 35

Lotus japonicus

Transformed rootlumox® film

M2 macrophages

Human monocyte derived macrophages

lumox® dish hydrobic

Macrophage

Human, primary

lumox® dishMacrophage

Murine bone marrow derived macrophage, primary

lumox® multiwell

MCF10A

Human breast cell line

lumox® multiwell

MDA-MB-231

Human mammary adenocarcinoma

lumox® multiwell

MDA-MB-231

Human mammary adenocarcinoma

lumox® dish 35 (94.6077.331)

MDM

Human monocyte-derived macrophages

lumox® dishMedicago truncatula

Transgenic root explantlumox® film

MEF

Murine embryonic fibroblasts

4-well lumox® detachable

MEF

Mouse embryo fibroblast cells transfected with Oct-4-GFP

4-well lumox® detachable

MH-S

Murine alveolar macrophages

lumox® multiwell 24

mESC line

Murine embryonic stem cell line 129/SVEV feeder-dependentlumox® multiwell 24

miPSC-RPE cell sheets

Retina pigment epithelium cell sheets derived from monkey induced
pluripotent stem cells

lumox® dish 35

Monocytes/Macrophages

Human, primary

lumox® dish hydrobic

Monocytes/Macrophages

Human, primary

lumox® dishMonocytes

Human, primary

lumox® film

MSC

Primary muscle derived cells

lumox® dishN2aMurine neuroblastoma cell line

lumox® multiwell 24

Neuroblastoma

Human neuroblastoma cells

lumox® multiwell 24

Neuronal hippocampal culture

Rat, primary

lumox® multiwell 24

NIH3T3

Murine embryonic fibroblast cell line

lumox® dish 35

NK Cells

Natural Killer, primary humanlumox® dish 94.6077.305

PBMC

Peripheral blood mononuclear cells

lumox® dishPC

Drosophilar polar cells

lumox® dish 94.6077.410

PC12

Rat pheochromocytoma cell line

x-well lumox®

pFB

Primary bovine fibroblasts

lumox® dish 35

PIXV

Pixuna Virus

lumox® dish 50 (94.6077.410)

8

Analysis / application

Literature / Source

Fluorescent microsocpy

Menzel, S. et al., Hindawi, BioMed Research International, Volume 2017, Article ID 5258196,
8 pages, https://doi.org/10.1155/2017/5258196

Phase microscopy

Customer information / SARSTEDT in-house test

Time-lapse microsopy

Yamamoto S. et al., PLOS ONE 2014, Vol. 9 Issue 7, doi:10.1371/journal.pone.0103502

Fluorescent microscopy

Puschmann, C., Application Note

Fluorescence and electron Microscopy

Nossol, C. et al., Histochem Cell Biol (2011) 136:103–115, DOI 10.1007/s00418-011-0826-y

Fluorescence and electron Microscopy

Nossol, C. et al., Histochem Cell Biol (2011) 136:103–115, DOI 10.1007/s00418-011-0826-y

Confocal microscopy

Kern, M. et al., Hindawi, Mediators of Inflammation, Volume 2017, Article ID 2748192, 13 pages

Confocal microscopy

Cristo F. et al., Stem Cell Research 25 (2017) 152–156

Fluorescent microsocpy

Freyer, N. et al., Int. J. Mol. Sci. 2017, 18, 1724

Cultivation

Eguchi, N. et al., Biochemical and Biophysical Research Communications 498 (2018) 729e735

Time-Lapse Recording Using Digital Holographic Microscopy

Janicke, B. et al., Cytometry Part A 91A: 460469, 2017

Real time phase contrast

Sun, Z. et al. 2018, Cancer Res. 2018 February 15; 78(4): 950–961.
doi:10.1158/0008-5472.CAN-17-1597

Time-Lapse Recording Using Digital Holographic Microscopy

Janicke, B. et al., Cytometry Part A 91A: 460469, 2017

Flow Cytometry

Matsuo, T. et al., ONCOLOGY LETTERS 14: 3071-3076, 2017

Cultivation

Krebs M. et al., The Plant Journal (2012) 69, 181–192

Cultivation

Bayer, C. et al., J. Virol. 2013, 87(1):67. DOI: 10.1128/JVI.01585-12.

Cultivation

Grimm, V et al., AEM, 2014, Vol.80 No 9 p. 2842–2850, doi:10.1128/AEM.04261-13

Cultivation

Wiese, M. et al., Infect. Immun. 2012, 80(4):1455. DOI: 10.1128/IAI.05972-11.

GC-MS, LC/MS-MS

Beatty, A. et al. 2017, DOI: 10.1158/1535-7163.MCT-17-0407

GC-MS, LC/MS-MS

Beatty, A. et al. 2017, DOI: 10.1158/1535-7163.MCT-17-0407

Cultivation

Bordag et al., Metabolomics 2016, 6:1, doi: 10.4172/2153-0769.1000164

Cultivation

Customer information / SARSTEDT in-house test

Live Imaging

Customer information / SARSTEDT in-house test

Live cell imaging

Rolf H., et al., PLoS ONE, 2012, Vol. 7 Issue 2 e32287, doi:10.1371/journal.pone.0032287

Cultivation

Gaus, L. Inaugural-Dissertation, "Charakterisierung des Expressions- und
Proliferationsverhaltens der STRO-1-positiven und -negativen Rosenstockperiostzellen
des Europäischen Damhirsches (Cervus dama), Medical faculty Georg-August-University
Göttingen, 2015

Fluorescent plate reading

Thywißen, A. et al., Front. Microbio. 2011, Vol. 2:96. doi: 10.3389/fmicb.2011.00096

Cultivation

Knöspe, F. 2012, Dissertation: Expansion of embryonic stem cells in 3D-bioreactors

Laser microdissection

Customer information / SARSTEDT in-house test

Cultivation

Bayer, C. et al., J. Virol. 2013, 87(1):67. DOI: 10.1128/JVI.01585-12

Cultivation

Neu C, et al., PLoS ONE 2013 8(6): e66898

Culture bags

Vogl, T., Application Note

Fluorescent microsocpy

Customer information / SARSTEDT in-house test

Confocal laser scanning microscopy

Customer information / SARSTEDT in-house test

Electron microscopy

Winkler, M. et al., SARSTEDT Application Note

Fluorescent microsocpy

Martens, M., SARSTEDT Application Note

Phase-contrast microscopy

Customer information / SARSTEDT in-house test

Cultivation

Figueiredo, A. et al., Data in Brief 14 (2017) 77–83

Cultivation

Bayer et al., J. Virol. 2013, 87(1):67. DOI: 10.1128/JVI.01585-12.

Concocal image stacks, Live Imaging

Torres, A. et al., Cell Death and Disease (2017) 8, e2814; doi:10.1038/cddis.2017.166

Fluorescent and bright field microsocpy

Wissel, K., SARSTEDT Application Note

Cultivation

Schwarting et al., PLOS ONE 2015,| DOI:10.1371/journal.pone.0116833

UV irradiation experiment

Sagripant, J. et al., Photochemistry and Photobiology, 2011, 87: 1369–1378

9Reference Guide

Cell type / tissue / organism

Description

Product

PMSC

Primary porcine muscle derived stem cells

lumox® dish 35

pOB

Primary bovine osteoblasts

lumox® dish 35

Pseudoalteromonas carrageenovora

Gammaproteobacteria

lumox® film

Pseudomonas sp.

Gammaproteobacteria

lumox® film

PtK2

Rat kidney epithelial cell line

x-well lumox®

Raji

Lymphoma cell line, humanlumox® multiwell

Retina ExplantRat retina explant culture

lumox® dish 35

Retina Explant culture

Zebrafish retina axon explant culture

lumox® dishRoot Hair/Root epidermal cells

Arabidopsis, tubular extensions from trichoblasts

lumox® dishScN2aMurine neuroblastoma cell line scrapie-infected

lumox® dish 50 (94.6077.410)

S. epidermidis

Bacteria S. epidermidis ATCC 35984

lumox® multiwell 24

Schizosaccharomyces pombe

Fission yeast strain

lumox® dishSerum

Mouse serumlumox® multiwell 384

SH-SY5Y

Human neuroblastoma cell line

lumox® multiwell 96

SK-MEL-5

Human melanoma celllumox® multiwell 96

SKX

Radiosensitive squamous cell carcinoma cell line

4-well lumox® detachable

SZ95

Human sebocyte cell line

x-well lumox®

T98G

Human glioblastoma cell line

lumox® dish 50

Tendon Culture

Bovine tendon culture

lumox® dish 35

THP1

Human monocytic macrophages

lumox® multiwell

THP-1

Human monocytic macrophages

lumox® multiwell 24

TJ356 worms

Caenorhabditis elegans

lumox® dishTracheal epithelium

Primary, porcine

x-well lumox®

TS cells

Tryphoblast stem cells

lumox® dish 35

U937

Human lymphoma cell line

x-well lumox®

VACV

Vaccinia Virus

lumox® dish 50 (94.6077.410)

Zooshikella sp.

Gammaproteobacteria

lumox® film

10

Analysis / application

Literature / Source

Fluorescent microsocpy

Customer information / SARSTEDT in-house test

Cultivation

Schwarting et al., PLOS ONE 2015,| DOI:10.1371/journal.pone.0116833

Biofilm culture

Lachnit, T. et al., FEMS Microbiol Ecol 84 (2013) 411-420

Biofilm culture

Lachnit, T. et al., FEMS Microbiol Ecol 84 (2013) 411-420

Fluorescent microsocpy

Dehne, H.-J., SARSTEDT Application Note

Fluorescent microsocpy

Welk, M. et al., SARSTEDT Application Note

Cultivation

Liu, H. et al., IOVS, October 2017, Vol. 58, No. 12, 5130

Time-lapse imaging

Customer information / SARSTEDT in-house test

Live imaging

Zárský, V. and Cvrcková, Methods in Molecular Biology, 2014, Vol. 1080, pp 195-199

Electron microscopy

Customer information / SARSTEDT in-house test

Live and dead fluorescence microplate readings

Svensson S. et al., Intern. J. of Nanomedicine 2014:9 pp. 775-794

Cultivation

Customer information / SARSTEDT in-house test

Fluorescence intensity measurement

Patil SS, Gentschev I, Adelfinger M, Donat U, Hess M, et al. (2012), PLoS ONE 7(10): e47472

Cultivation

Taylor, J. et al., Faraday Discuss., 2017, 205, 409

Time-Lapse Recording Using Digital Holographic Microscopy

Janicke, B. et al., Cytometry Part A 91A: 460469, 2017

Fluorescent microsocpy

Kraft, S.D. et al., New Journal of Physics 12 (2010) 085003 (12pp)

Fluorescent microsocpy

Seltmann, H. et al., SARSTEDT Application Note

Real time phase contrast

Sun, Z. et al. 2018, Cancer Res. 2018 February 15; 78(4): 950–961

Cultivation

Schwarting et al., PLOS ONE 2015,| DOI:10.1371/journal.pone.0116833

Live Cell Imaging

de Morrée, A. et al., The Journal of Biological Chemistry Vol. 288, No. 20, pp.
14147–14157, 2013

Fluorescent plate reading

Thywißen, A. et al., Front. Microbio. 2011, 2:96. doi: 10.3389/fmicb.2011.00096

Live Imaging

Keshet, A. et al., Mol Genet Genomics (2017) 292:1341–1361

Bright field microscopy

van de Wal, R., SARSTEDT Application Note

Live Cell Imaging

Hesse, M. et al., Nat. Commun. 3:1076, doi: 10.1038/ncomms2089 (2012)

Fluorescent microsocpy

Wagner, D., SARSTEDT Application Note

UV irradiation experiment

Sagripanti, J. et al., Photochemistry and Photobiology, 2011, 87: 1369–1378

Biofilm culture

Lachnit, T. et al., FEMS Microbiol Ecol 84 (2013) 411-420

11

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lumox® & x-well Technology

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Cell Cultivation, Cryogenic Storage, Filtration, Liquid Handling

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Growth Surface References

miniPERM®

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Bioreactor for protein production

T

Which options do I have for the cultivation of my cells?

C

TestedSterile/ DNA-/ DNase-/
RNase-/ Pyrogen-free/
non-cytotoxic

Sarstedt TC Inserts

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CryoPure freezing system
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non-mutagenic

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