2008日韓ジョイントシンポジウム
韓国動物繁殖学会の会長建国大学のHoon Taek Lee教授から、日本繁殖生物学会の渉外理事である永井宛てに、韓国動物繁殖学会年次大会で開催される、下記のタイトルの「第４回日本繁殖生物学会と韓国動物繁殖学会のジョイント・シンポシウム」でのポスター発表を受け入れるとのメールがまいりました。そこで、日本繁殖生物学会の会員でポスター発表を行いたい方は、どしどし参加して頂きたいと思います。

シンポジウムのタイトル：動物繁殖バイオテクノロジーにおける最近の進展“Recent Advances in Anima Reproductive Biotechnology”
場所：建国大学：韓国ソウル
日時：２００８年６月２０日
アブストの形式：MSワード, フォント Times New Roman, 11 ポイント, ２行 , A4 一枚（別紙に例を添付）
アブ スト締め 切 り ： ２ ００ ８年 ５ 月 ２ ５ 日
アブスト送付先：Yong-Mahn Han、E-mail; ymhan@kaist.ac.kr
参加費：50 米ドル（韓国動物繁殖学会年次大会本大会：６月２０日‐２１日への参加費でありコーヒー・昼食・懇親会費を含む；現金で当日支払い）.
若手企画の平尾雄二会員が、日本側の事務局を努めております。
シンポジウムの内容につきましては、現在、両事務局で詰めているところです。最新版を別紙に示します。最終版については、追って情報を流しますが、プレナリーセッションとセッションI〜IIIがあります。現在予定されている日本側スピーカーは、下記の通りです。

Plenary Session
Dr. Eimei Sato (Tohoku University)
Session I: Embryo & Development
Dr. Masashi Takahashi (National Agricultural Research Center for Kyushu
Okinawa Region)
Dr. Tamas Somfai (National Livestock Breeding Center)
Session II: Reproductive Endocrinology
Dr. Takashi Matsuwaki (The University of Tokyo)
Dr. Yoshihisa Uenoyama (Nagoya University)
Session III: Animal Cloning
Dr. Satoshi Kishigami (Kinki University)
日本繁殖生物学会渉外理事
永井 卓（畜産草地研究所）

The 4th JSAR-KSAR Joint Symposium“ Recent Advances in Anima Reproductive Biotechnology”

June 20-21, 2008, in Konkuk University.
June 20 (Fri)
08:30-09:20

Registration

09:20-09:30

Opening ceremony (Presidents of JSAR and KSAR)

Plenary Session
09:30-10:10

Hoon Taek Lee, Ph.D., Konkuk University
Recent Progress in Animal Reproduction in Korea

10:10-10;50

Eimei Sato, Ph.D., Tohoku University
Recent Progress in Animal Reproduction in Japan

10:50-11:10

Coffee break

11:00-13:00

Poster Session I (KSAR)

12:00-13:00

LunchSession I : Embryo & Development
13:00 -13:25

Chairman: JSAR, KSAR

Masashi Takahashi, Ph.D., National Agricultural Research Center forKyushu Okinawa Region
RNA interference : a tool for gene regulation in embryonicdevelopment and application

13:25-13:50

Kweon Yu, Ph.D., KRIBB.
Study of metabolic syndrome with the Drosophila model system:sNPF regulates insulin signaling.

13:50-14:15

Tamas Somfai, Ph.D., National Livestock Breeding Center
Development of polyspermic embryos in IVP systems of farmanimals

14:15-14:40

Jeong Mook Lim, DVM & Ph.D., Seoul National University.
Stem cell establishment without undertaking nuclear transfer.

14:40-15:00

Coffee break

Session II : Reproductive Endocrinology Chairman : JSAR, KSAR
15:00-15:25

Yoshihisa Uenoyama, Ph.D., Nagoya University

15:25-15:50

Kyung-Ah Lee, Ph.D., Pochon CHA University.

15:50-16:15

Takashi Matsuwaki, Ph.D., The University of Tokyo
Protective effects of glucocorticoids on the reproductive function
under stress conditions

Session III : Animal Cloning

Chairman : JSAR, KSAR

16:15-16:40

Il Keun Kong, Ph.D., Gyeongsang National University.

16:40-17:05

Satoshi Kishigami, Ph.D., Kinki UniversitySomatic cell nuclear transferwith HDACi

17:05-17:30

Jin-Hoi Kim, Ph.D., Kon-Kuk University
Analysis of hypoplastic umbilical cords and placenta from somatic
cell nuclear transfer-derived piglets: implications for early postnataldeath.

17:30-17:40

Closing remark

17:40-20:00

Welcome party

June 21 (Sat)KSAR-student competition and Poster session II

（例）Solid Surface Vitrification of Porcine Oocytes Effect of Cytoskeletol Stabilizer,
Cryoprotectant and Dilution Method
Mukesh Kumar Gupta, Sang Jun Uhm and Hoon Taek Lee
Bio-Organ Research Center, ARRC, Konkuk University, Seoul, Republic of Korea
Cryopreservation of normal, lipid containing pig oocytes has met with limited practical success.
This study used solid surface vitrification (SSV) for immature GV and mature MII porcine
oocytes and evaluated the effects of pretreatment with cytochalasin B (CB), cryoprotectant type(DMSO vs EG vs DMSO+EG) and dilution method (Two- vs Single-step). The vitrified oocytes
were assessed for survival rates, nuclear maturation and developmental capacity following invitro fertilization (IVF). The results showed that both GV and MII stage porcine oocytes could
be successfully vitrified by SSV. Following cryopreservation in EG by SSV more than 60% of
GV and MII stage porcine oocytes remained intact. This was not significantly improved bycytochalasin. GV oocytes vitrified in DMSO had significantly lower nuclear maturation rates(31%) than GV oocytes vitrified in EG (51%) or EG+DMSO (53%) (P<0.05). The use of two
step dilution gave better survival than single-step dilution. Although the survival rates were high,as assessed by morphological appearance, FDA staining and cleavage, the blastocyst rate
(3-9%) was significantly lower than for the non-vitrified controls (20%). This shows that a very
simple, rapid, procedure allows normal, lipid containing, porcine oocytes vitrified at either theGV or MII stage to fertilize and develop into blastocysts in vitro. This work was supported by
the Research Project on the Production of Bio-Organs (No. 200503030201), Ministry ofAgriculture and Forestry, and Biogreen 21, RDA, Republic of Korea.
[Key words: solid surface vitrification, oocyte, porcine]