Ergonomic Pipetting for Problem Liquids!

ContentsPage

1-INTRODUCTION

32-PARTS CHECKLIST43-

DESCRIPTION

44-

OPERATING RANGES AND MATERIALS

55-FITTING THE CAPILLARY AND PISTON56-

SETTING THE VOLUME

77-

PIPETTING88-

GLP FEATURES

99-

TROUBLESHOOTING

910 - CLEANING AND DECONTAMINATION

1011 - SPECIFICATIONS

12WARRANTY

14EC DECLARATION OF CONFORMITY

15Before use, please carefully read Warranty
page 14 of this User's Guide!

1 - INTRODUCTION
Congratulations on purchasing your new MICROMAN® Epipette. MICROMAN E is suitable for many different types
of application, including pipetting volatile, viscous, dense,or high surface tension liquids. It is a quality product from
Gilson, which is fully compliant ISO 8655, CE labeled.
The six models available allow for precise and accurate
pipetting of viscous, dense and volatile liquids. They alsoenable contamination-free pipetting, avoiding vapor
contamination and cross-contamination. M25E, M50E
and M250E, with thin and long CPs are especially suitedfor use with long or narrow vessels.
Enjoy the following features:- MICROMAN E pipettes are equipped with a positive
displacement mechanism isolating the aspirated
liquid from the pipette body. The positive displacement mechanism prevents the sample-to-sample
contamination that can result from the aerosol effect.
- MICROMAN E uses disposable capillaries and pistons. These are the only parts to be in touch with theaspirated liquids, and replacement thereof will provide
absolute protection against contamination caused
by carryover between samples or reagents. Simply
replace the capillary and piston between assays; they
are ejected automatically and simultaneously, thus
avoiding any risk for the operator.
- MICROMAN E is equipped with a direct reading
volumeter that allows precise adjustment of therequired volume. MICROMAN E is permanentlycalibrated.
At the manufacturing stage, the capillary and piston
are positioned automatically, and therefore require
no further calibration.
- MICROMAN E requires no lubrication or maintenance,
due to the nature and quality of the materials used inits component parts.

23

2 - PARTS CHECK LIST

4 - OPERATING RANGES AND MATERIAL

Just take a moment to verify that the following itemsare present:

Available Models


MICROMAN E,User's Guide,
Safety bag,
Adhesive id-tags (strip of 6),
Capillary pistons (10),
Certificate of conformity(including barcode sticker).

magnifier with volume range
for aspirating and dispensing.

2 Body or handle.
3 Capillary.
 Piston.
 Shaft.
 Thumbwheel for

 Volumeter.
 Volume unlock button.
 Data matrix.

4







8





setting the volume.

Volume Range

M10E
M25E
M50E
M100E
M250E
M1000E

1 µL to 10 µL
3 µL to 25 µL
20 µL to 50 µL
10 µL to 100 µL
50 µL to 250 µL
100 µL to 1000 µL

Materials

3 - DESCRIPTION
1 Color-coded push-button andModel

Model
Spring Clamp
Shaft				Body(B)

M10E
M25E
M50E
M100E
M250E
M1000E

SS
SS
SS
SS
SS
SS

PVDF/PPPVDF/PPPVDF/PPPVDF/PPPVDF/PPPVDF/PPCP

Capillary(C)Be Alloy
Be Alloy
Be Alloy
Be Alloy
Be Alloy
Be Alloy

PBT
PVDF
PVDF
PBT
PVDF
PVDF

Abbreviations
SS = Stainless Steel
Be = Beryllium
PBT = Polybutylene Terephthalate
PP = Polypropylene
PVDF = Polyvinylidene Fluoride

Piston(P)CP10 Polypropylene Polyester
CP25 Polypropylene Polyester
CP50 Polypropylene Polyester
CP100 Polypropylene Polyethylene
CP250 Polypropylene Polyethylene
CP1000 Polypropylene Polyacetal

5 - FITTING THE CAPILLARY AND PISTON
Never lubricate the capillary holder, capillary,or any other part of the pipette. If the capillarytends to slip off the capillary holder, clean thecapillary holder with ethanol, using a medical
wipe or similar soft tissue.

5Make sure the capillary piston (CP)model matches the MICROMAN E
you are about to use:
CP25, CP50 and CP250 Bulk
The capillaries and pistons are delivered in two separate boxes. To fit acapillary piston, proceed as illustrated:
1) Take care not to damage thesealing tip  when handlingthe piston .
2) Select a piston  and slide
it into the capillary .
3) Gently push the capillary
until it snaps onto the capillary holder.
4) Slowly press the pushbutton to the first stop toattach the stem . You
should definitely feel andhear a slight click.

CPMICROMAN E
model

CP10

M10E

CP25

M25E

CP50

M50E

CP100

M100E

CP250

M250E

CP1000

M1000E66 - SETTING THE VOLUME
The volume of liquid to be aspirated is set using thevolumeter. The volumeter consists of three number dials,
which are read from top (most significant digit) to bottom(least significant digit). A marker is used to set exact orintermediate volumes using the scale on the bottom dial.

2
3The dials are colored either black or red to indicate theposition of the decimal point, according to the model (seeexamples).



CP10, CP25, CP50, CP100,
CP250 and CP1000 Tipack
Capillary pistons (CP) aredelivered ready to use. To fit
a capillary piston, proceedas follows:
1) G e n t l y p r e s s t h e
MICROMAN E onto thecapillary piston until it
is firmly seated on theMICROMAN E .
2) Pick up the capillary fromthe rack 2.
3) Slowly press the push-button to the first stop until you feel and hear a slightclick 3.
4) Do not release the push-button and pipette yoursolution.

3

M10 E
068M100
M25EE
068

M10
M50
M25EE
3066
88

M100
M250
M50EEE
103
66
88

M1000
M250EE017658

M1000E

6.8 µL68 µL
6.8

6.8 µLµL
36.8
6.868µLµLµL 0.75
16836.8
168 mLµL

0.75 mL

Model

Color of volumeter numbers
Increment
BlackRedM10E, M25E, M50EµL

0.1 µL0.02 µL

M100E, M250E

µL

none

0.2 µL

0.1 and 0.01 mL

mL2 µL

M1000E075

The volume is set by turning the thumbwheel slowly to reach the required setting.
For small volume changes, directly andslowly turn the thumbwhell.

2To obtain maximum accuracy whensetting the volume, proceed as follows:- when decreasing the volume setting,slowly reach the required setting, making sure not to overshoot the mark.
7

- when increasing the volume setting, pass the required
value by 1/3 of a turn and then slowly decrease thevolume to reach the required setting, making sure not
to overshoot the mark.
For large volume changes, proceed
as follows :8 - GLP FEATURES

7 - PIPETTING- Press the push-button to thefirst stop (1),- Immerse the capillary 2 mm
into the liquid,- Slowly release the pushbutton to draw up the liquid(top position),- Wipe any liquid from the outside of the capillary, taking care
not to touch the orifice.

Top1
2

Dispensing- Place the end of the capillary against the inside wallof the recipient vessel,- Press the push-button slowly to the first stop,- Keeping the push-button depressed, move the capillary away from the sidewall,- Withdraw MICROMAN E from the vessel and release
the push-button.
8Press the push-button to the first stop (1), then press
down harder to the second stop (2); at this point thepiston and capillary are ejected simultaneously.
MICROMAN E is automatically calibrated by correctly fitting acapillary piston. Once the volume is set, measurements will beaccurate and precise.

1) Push the unlock button counter.
2) When decreasing the volume
setting, approach the requiredsetting, by 1/3 of a turn.
When increasing the volume
setting, pass the required settingby 1/3 of a turn.
3) Release the unlock button counter.

Aspirating

Ejecting the capillary piston- No need to touch disposables (capillary piston).
- Serial Number: engraved on pipette body.
- Barcode: on the box and with the certificate (can betransferred).
- ID Tag (Application or User).
- Color-coded push-button.
- Color-coded magnifier with volume range.
- Data matrix engraved on pipette body.
- Gilson certificate of conformity according
to ISO 8655.

9 - TROUBLESHOOTING
You may be able to identify and correct the problem
by referring to the following table. If you cannot solve
the problem, please contact your Gilson representative.
Leaks:
Replace the capillary and piston.
No stroke:
The piston is not properly fitted into the clamp; checkthat the piston is seated correctly (refer to pages 5-7).
Without any capillary piston, press the push-button to
the first stop, then press down harder to the second stop.
Difficult to fit a capillary:
Clean the capillary holder with ethanol. Without any
9capillary piston, press the push-button to the first stop,then press down harder to the second stop.
Inaccuracy:
Make sure the capillary is correctly mounted on thecapillary holder (refer to pages 5-7).
Imprecision:
Replace the capillary and piston.
Difficult to set the volume:
As this suggests internal damage to the pipette you
should contact your Gilson representative.
If the problem persists...
Contact your Gilson representative.

10 - CLEANING AND DECONTAMINATIONMICROMAN E is designed so that parts normally incontact with liquid contaminants are easy to clean anddecontaminate.		
Cleaning
Clean the pipette with soap solution prior to decontamination.
External
1) Wipe the entire pipette with a soft cloth or lint-freetissue impregnated with soap solution to remove anystains. If the pipette is very dirty, a brush with soft
plastic bristles may be used.
2) Wipe the entire pipette with a soft cloth or lint-freetissue impregnated with distilled water.
3) Leave the parts to dry by evaporation or wipe themdown with a clean soft cloth or lint-free tissue.
Before returning any pipette, ensure that it is completely free of chemical, biological, or radioactivecontamination. Use the safety bag provided by
Gilson.

10Internal
Only the following components should be immersed ina decontaminant solution: clamp assembly, return springand capillary holder (shaft).
1) Unscrew the capillary holder (shaft).
2) Remove the clamp assembly and return spring fromthe shaft.
3) Set aside the body (handle) in a dry and secure location.
4) Clean the individual components using an ultrasonicbath (for 20 minutes at 50 °C) or with a soft cloth andbrush. Small round brushes with soft plastic bristles
may be used to clean the interior of the shaft.
5) Rinse the individual components with distilled water.
6) Leave the parts to dry by evaporation or wipe themdown with a clean soft cloth or lint-free tissue.
7) Reassemble the pipette.
Decontamination
You may refer to the Decontamination Procedure
LT802288 available at www.gilson.com.
Chemical Decontamination
Clean the pipette prior to decontamination. Full detailsof recommended decontamination procedures for Gilson
pipettes are available from your supplier. Whichever otherdecontaminant you use, check with the supplier that theproduct is compatible with the materials used for thecomponent parts of the pipette, and that does not attack
either of the following plastics and metals: polyester,PVDF (polyvinylidene fluoride), PP (polypropylene), PBT(polybutylene terephthalate), PC (polycarbonate), SS(stainless steel), and BE Alloy (beryllium).
Non-immersible Parts1) Wipe the body (handle) of the pipette with a soft
cloth or lint-free tissue impregnated with the chosen
decontaminant.
2) Wipe the body (handle) of the pipette with a soft clothor lint-free tissue impregnated with distilled water orsterilized water.
11

Immersible Parts
Only the following components should be immersed ina decontaminant solution: clamp assembly, return springand capillary holder (shaft).
1) Unscrew the capillary holder (shaft).
2) Remove the clamp assembly and return spring fromthe shaft.
3) Set aside the body (handle) in a dry and secure location.
4) Immerse the components in the decontaminantsolution or wipe them down according to theinstructions provided by the manufacturer or supplierof the decontaminant.
5) Rinse the individual components with distilled orsterilized water.
6) Leave the parts to dry by evaporation or wipe themwith a clean lint-free tissue or a soft cloth.
7) Reassemble the pipette.

11 - SPECIFICATIONS• Ten measurements for each test volume, which arenominal volume, 50% of nominal volume, and theminimum or 10% of nominal volume.
Performance Tests
Each pipette is inspected and validated according to theGilson Quality Assurance System. Based on extensive
historical data, manufacturing conditions and expertise,and in compliance with ISO standards relative to statisticalprocess control, the assurance level of this instrument
performing to specifications is 99.8 %. Specifications relyon quality and consistency for the whole pipetting system
and are guaranteed only when the pipette is used withGilson capillary pistons.
Gilson Maximum Permissible Errors
ModelVolume(Reference)(µL)
		Maximum Permissible Errors
GilsonISO 8655
SystematicRandom
SystematicRandom
error (µL)
error (µL)
error (µL)
error (µL)

M10E
Min
(FD10001)		
Max.

1510± 0.09± 0.10± 0.15

 0.03
 0.03
 0.06

± 0.2
± 0.2
± 0.2

 0.1
 0.1
 0.1

MICROMAN E is a high quality pipette that offers excellent accuracy and precision. The figures provided in the“ Gilson Maximum Permissible Errors ”table were obtainedusing Gilson CPs. These figures are only guaranteed whenusing genuine Gilson CPs.

M25EMin.
(FD10002)		
Max31025± 0.25
± 0.27± 0.30 0.08
 0.08
 0.10± 0.7± 0.7± 0.7

 0.3
 0.3
 0.3

M50E(FD10003)

Min.
Max.

20
50

± 0.34± 0.70 0.20 0.30

± 0.7
± 0.7

 0.3
 0.3

Each pipette is inspected and validated by qualified
technicians according to the Gilson Quality System.

M100E
Min.
(FD10004)		
Max.

10
50
100

± 0.50± 0.75± 1.00 0.20 0.30 0.40

± 1.5
± 1.5
± 1.5

 0.6
 0.6
 0.6

Gilson declares that its manufactured pipettes complywith the requirements of the ISO 8655 Standard, bytype testing. The adjustment is carried out under strictly
defined and monitored conditions (ISO 8655-6):• Basis of adjustment, Ex.
• Reference temperature, 20 °C• Relative humidity, 50%• Barometric pressure, 101 kPa• Use of distilled water grade 3 (ISO 3696)

M250E
Min.
(FD10005)		
Max.

50
100
250

± 1.50± 1.70± 2.50 0.30 0.30 0.50±6±6±6

 2.0
 2.0
 2.0

M1000E
Min.
(FD10006)		
Max.

100
500
1000± 3.0
± 5.0
± 8.0

 1.6
 2.5
 4.0

± 12± 12± 12

 4.0
 4.0
 4.0

12

The data given in the table conform to the ISO 8655-2 Standard.

13WARRANTY
Gilson warrants this pipette against defects in material under normal
use and service for a period of 12 months from the date of purchase.
This warranty shall not apply to pipettes which are subject to abnormal
use and/or improper or inadequate maintenance (contrary to therecommendations given in the User’s guide), including, but not limited
to pipettes which have been subjected to physical damage, improper
handling, spillage or exposure to any corrosive environment. This warranty shall also be void in the event pipettes are altered or modified
by any party other than Gilson or its designates. Gilson’s sole liability
under this warranty shall be limited to, at Gilson’s sole option, repair orreplacement of any defective components of pipettes or refund of thepurchase price paid for such pipettes.
THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBYDISCLAIMS ALL OTHER WARRANTIES, WHETHER EXPRESS OR IMPLIED,INCLUDING ANY WARRANTIES OF MERCHANTABILITY AND ANY
WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE, UNDER NO
CIRCUMSTANCES SHALL GILSON BE LIABLE FOR ANY CONSEQUENTIAL,PUNITIVE, INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF ANY
BREACH OF ANY EXPRESS OR IMPLIED WARRANTY.

EC DECLARATION OF CONFORMITY
The company,

GILSON S.A.S.
19, avenue des Entrepreneurs
BP. 145 - 95400 Villiers-le-Bel (France)
Tel.: +33(0) 1 34 29 50 00
Fax: +33(0) 1 34 29 50 20
www.gilson.com
Hereby certifies under its sole responsibility that the products
listed below:

MICROMAN® E
M10E, M25E, M50E, M100E, M250E, M1000E
Positive displacement Capillaries/Pistonscomply with the requirements of the following European
Directives:

98/79/EC*on In Vitro Diagnostic Medical Devices* Annex III, self-declared

Villiers-le-Bel, June 20th, 2014

E. Chamault
General Manager

14

H. Le Dorze
Quality Manager

15

LT801547/A - ©2014 Gilson SAS All rights reserved
June 2014
EnglishPrinted in France
Specifications subject to change without notifications - errors omitted.

www.gilson.com
sales@gilson.com
service@gilson.com
training@gilson.com
Gilson, Inc. World Headquarters
3000 Parmenter Street I P.O. Box 620027 I Middleton, WI 53562-0027, USA
Tel: (1) 800-445-7661 or (1) 608-836-1551 I Fax: (1) 608-831-4451
Gilson S.A.S.
19, avenue des Entrepreneurs I BP 145, F-95400 Villiers-le-Bel, France
Tel: +33 (0) 1 34 29 50 00 I Fax: +33 (0) 1 34 29 50 20