EM-Menu 4
Advanced TEM image processing package
EM-Menu 4 is a powerful image acquisition and processing software
package for the specific tasks in electron microscopy such as supporting
different remote controls for reading the TEM parameters and controlling
the TEM in its main functions. The versatile and flexible user interface
developed for Windows XP allows efficient data acquisition.

Camera Configuration Manger:

They can be moved, copied, renamed

10 000 x 10 000 pixels or even more.

The Camera Configuration Manager links

or deleted to prepare optimal structures

Using this image, the stage can beall selectable camera settings (e.g. size,before saving them to hard disk.

moved with a typical precision of aroundbinning exposure time, shutter settings)

1 μm (depending on the stage type).

to a logical camera. Assigning them

Navigation:

For defining the various areas, e.g. forto different buttons or viewports offers

The module NAVIGATION was speciallytomography or single particles dataa rapid change between settings fordeveloped for low-dose applications, soacquisition, further navigation at higher

search and acquisition.

as to avoid any unnecessary exposure

magnifications is done with image shift.

during searching or focusing. It allows
Image Manager:

the collection of an automatically

The Image Manager organizes theacquired tiled image of the complete

acquired images in tree-like directories.

grid. The size of the image is typicallyFeatures and benefits
EM-Menu 4:

Operations in Fourier space:

Shutterbox II:• The modern solution for TEM image• Power spectra• Interface between cameras and TEM

acquisition, processing and analysis• Split scaling• Supports pre-specimen and postspecimen• Multiple viewports with zooming/• Zooming and panning

shutter (beam blanker andpanning function• Linescanexposure shutter)• Main controls on the dialog bar or• Low-/high-/band-pass*• Variable pre-exposure time:

freely placeable

1 ms - 65 sec• Image manager supports tree andFile input/output:• Minimum opening time for mechanicalseries structures• 8 bit and 16 bit TIFF images

shutters: 100 ms - 2 sec• Layout manager• mrc format• em format

System requirements:

Camera configuration:• PC workstation• Logical cameras

Scripting and TEM control:• Min. 1 GB memory• Flatfield database• Scripting in VisualBasicScript or(2 GB recommended)

JavaScript via standard software• Monitor with min. 1280 x 1024

Image acquisition:interfacepixels, 1600 x 1200 pixels• Single snapshot• Passive remote control interfaces forrecommended• Continuous readout with live flat-automated readout of image relevant• Windows XP

fielding, FFT calculation and/or linescan• Video recorder** = option
TEM parameters• Electron dose calculation

Image processing & analysis:• Magnification Selector for TEMs

• Single and average line-scans inwithout remote control

arbitrary directions• Active remote control*• Image statistics• Spot scan and tiling*• Histogram• Autofocus*• Navigation*• Tomographic acquisition of tilt series*• Single Particle Data Collection*

Tiling:Tiling extends thefield-of-view of thecamera. Automatic
acquisition and pixelaccurate assemblywith compensationof intensity variations
generate a large
seamless image
from a set of single
exposures.

Tiled image (4563 x 4431 pixel), composed of 36 images (1024 x 1024 pixel each) of a freeze fractured yeast cell (courtesy of Reinhard Rachel, University Regensburg)

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