SURFE²R N1In-depth transporter researchSURFE²R N1

Unveiling Membrane Transport
through SSM-based Electrophysiology
Nanion’s SURFE²R (surface electrogenic event

Any membrane can be accessed for recordings,reader) instruments allow for functional and direct

ranging from isolated membrane vesicles fromrecordings of electrogenic membrane transportnative tissues or cultured cells to transportersfrom symporters, exchangers, uniporters and pumps,

reconstituted into proteoliposomes.

and from ligand-gated and leak ion channels.
It’s easy to use, efficient, and unlocks completely
SURFE²R N1 facilitates in-depth functional

new possibilities in transporter research andcharacterization of electrogenic membrane

screening.

transport in real-time with high sensitivity and hightemporal resolution.

SURFE²R N1

Key features at a glance
1

Functional transporter recordings
in real-time

5

Pre-programmable, automatedrecordings

2

No need for labels, radioactivity
or fluorescence

6

Broad range of validated
transporter targets

3

High sensitivity & temporal resolution

7

Easy to learn and use — even forstudents

4

Access any membrane with only
0.1–1 µg protein per sensor required

8

Up to 150 datapoints per day

SURFE²R N1

SURFE²R recording principles
Membrane fragments from cell culture, tissue or

resulting in a gradient across the membrane. The

liposomes are adsorbed onto a solid supported

resulting electrogenic transport is measured as amembrane (SSM) on a gold coated sensor

transient, capacitive current which decays until

chip. Approximately 0.1–1 μg of membrane isa new electrochemical equilibrium has beenrequired to cover the 3 mm sensor electrode,reached. The size and shape of the current reveal

corresponding to ca 109 transporter molecules.

information about numerous biophysical andMembrane transport, i.e. charge translocation,

pharmacological transporter properties like theis triggered by rapidly perfusing the sensor withspeed of transport, coupling ratio or substrate

a substate or an altered ionic composition

affinity.

SAutomation
S

Automated measurements usingInjector

up to 53 solutions.
Up to 150 data points per day (SURFE²R N1).
Up to 10.000 data points per day (SURFE²R 96SE).

SubstrateSS

Compounds

S

Substrate and inhibitor assays; forhydrophobic compounds and metal

Electric read-out
No labels, direct measurements.
Real-time and immediate responses.

ions, negative controls are essential.

AS

High target range
SampleSymporters, exchangers, ATPases,
channels.

Capacitive read-out

Sample flexibility

Voltage-control throughionophores.

Native membranes or proteoliposomes.
Low sample consumption <0.1 µg
protein per sensor.
High protein density may be required.

Robust and reliable
High sensor stability.
Multiple measurements over hours.
High success rates andreproducibility.

SensorHigh sensitivity
Huge sensor surface.
Targets down to transport rates of0.1 s−1. Detects even conformationaltransitions and binding.

SURFE²R N1Explore the possibilities of fullexperimental freedom
Normalized current

Metformin

140 mM Na+ intern

EC50 = 0.3 mM

100 μM Ca2+ extern

Vmax = 770 pA

20 mM Ca2+ intern

220 mM Na+ extern

1TEA
EC50 = 4.6 mM
Vmax = 1.5 nA

01 nA
1−50.001

0.1

Substrate concentration [M]250 msTransport assays

Influx and efflux assays

Substrate specificity (Vmax, EC50).

Symmetry of transport.

Normalized current

IC50 = 580 μM

100Time resolution
Binding in GlcP
Transport in XylE

Control
50100 μM
500 μM
1 mM

Norm. nA

01−70.0001

50 ms

0.1

LyZ concentration [M]Compound assays

Substrate binding assay

Inhibition and positive modulation.

Pre-steady state currents (k+, k −).

I [norm]
XylE

1.0FucP

LacY

4.6

fof1 ATPase

fof1 ATPase

H+ pumping

ATP synthesis

ATPout/ADPin

1 nA
250 ms200 ms

6.3 7.5

9.4

10.9Compl. I/III/IV

10.6

812

1 nA
ΔADP

Compl. IV

100 msCompl. II/III

1 nA
250 mspH04

ΔATP

0.5 nA

7.70.5ANT
ADPout/ATPin

ΔNADH

250 pA
Δcyt.cred1 nA1s

Δcyt.cox

200 msComparative assays

Multiple targets in one experiment

Mutants, orthologues, isoforms.

Serial recordings from the same sample and sensor.

SURFE²R N1Broad range of validated targets
Primary active
transporters / Ion pumps

Uniporters+ Symporters + Exchangers

ATPases

Inorganic ionsAmino acids + peptides

NaK-ATPase

NhaA/B (2Na+/1H+)

PepT1/PepT_St (di-/tri-peptides)

HK-ATPase

NhaP/NHA2 (1Na+/1H+)

YdgR/DtpA (tri-peptides)

SERCA (Ca2+)

NCX1/mjNCX (3Na+/Ca2+)

YhiP/DtpB (di-/tri-peptides)

v-ATPase (H+)

ecClc/Clc-3/5/7/K (Cl −/H+)

PutP (Na+/proline)

FoF1-ATPase (H )NirC (NO /H )Kdp-ATPase (K )afAmt1-3/ksAmt5/ecAmtB (NH )GltTK (Na+/aspartate)

CopA (Cu )NeRh50 (NH )EAAC1 (3Na+/glutamate)

ATP7A/B (Cu )SulP/DauA (bicarbonate)

PAT1 (H+/proline)

ATP8A2 (Flippase)

NIS (2Na /I )ArcD (arginine/ornithine exchange)

VrPPaseNaPi-IIb (Na+/PO32−)

CAT-IIB (cationic amino acids)

MntH2 (Mn(II), Zn(II), Co(II), Cd(II))

GlyT1/2/B1 (Na+/glycine)

YiiP (metals)

B0AT2 (Na+/neutral amino acids)++2+−
2GltP (Na+/glutamate)

++
4+
42+

Redox-driven ion pumps+−

Cystin Transporter

Complex I
Respiratory chain complex I/III
Respiratory chain complex II/III

SugarsOther organic ions

Cyctochrome c-oxidase

SGLT1/2 (Na+/glucose)

OCT1/2 (organic cations)

Respiratory chain complexes I/III/V

MelB (Na+/melibiose)OATP1B1 (organic anions)

LacY (H+/lactose)

CNT1 (nucleosides)

FucP (H+/fucose)

ANT (ADP/ATP exchange)

XylE (H+/xylose)

GAT1 (Na+/GABA)

GlcP (glucose)

BetP (betaine)

STP10 (H+/glucose)

CHT (choline)

Light-driven ion pumps
Bacteriorhodopsin (BR)Rhodopsin OR1
Rhodopsin-2 (KR2)Halorhodopsin (HR)
Acerhodopsin
Channelrhodopsin 1/2 (ChR)

LicB (choline)
NupC (H+/nucleoside)
PurT (H+/purine)
NaCT (Na+/citrate)
SugE/Gdx (guanidinium)
EmrE (small drugs)

Channels + Pores

MCT13 (monocarboxylates)
PfCRT (cloroquine/CQ)
DgoT (H+/galactonate)

Gramicidine
P2X2nAChR
A/M2UCP1 (Slc25a7)
TRPC5
TRPA1
AQP6
KtrAB
TMEM175

Malate/lactate exchange
Bicarbonate transportSURFE²R N1The SURFE²R N1 includes• Turn-key, stand-alone system• PC and monitor with integrated recording and analysis software• SURFE²R N1 Sensor 3 mm for optimal signal-to-noise• SURFE²R N1 Sensor 1 mm for high temporal resolution• Integrated computer with Windows and SURFE²R N1 Control software• The SURFE²R Stimulating Optical Lid SOL (optional)• Ph.D. level assay support and highly qualified technical support• 1 year warranty with further optional comprehensive service plans available

Specifications
Data output

Signal amplitude: 0.1–10 nA, detection limit: 50 pA, noise: 20 pA

Low noise amplifier

Gain: 108 –1011 V/A, resolution: −10 V to +10 V (1.2 mV steps),
RMS noise: 0.5– 22 pA, low pass: 480 Hz, rise time: 0.7 ms

Measuring electrodeRe-usable gold coated sensor chip with 1 or 3 mm diameter

Reference electrodePlatinum electrode pin in measurement chamber

Integrated computer

Win11, SURFE²R N1 Control (data recording and analysis software)

Fast solution exchange

200 µl/s, continuous flow: 0.5– 6 s, time resolution: 5– 30 ms

Fluidic components

2 pumps, fast switching valve, tubing cassette, IonJet with Y-fluidic

Solution handling

Autosampler with 53 positions, each ~10 ml and2 teflon reservoirs, each ~70 ml

Solution consumption

Volume per measurement/substrate: ~300 µl (inhibitor: 1–2 ml)

accelerate your researchnanion Europe
info@nanion.dephone: +49 89 2190 95 - 0
www.nanion.denanion USA
info@naniontech.com
phone: 1-888-9-NANION
www.naniontech.com
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andy.di@nanion.cn
phone: +86 10 82 17 6388www.nanion.cn
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phone: +81 3 6457 8773
www.nanion.jp

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representative, visit our website:www.nanion.de