2009B1842                          BL40B2໬⢝ရᇶ๣ࡀᙧᡂࡍࡿᚤ⣽ᵓ㐀࠾ࡼࡧゅᒙᵓ㐀࡟୚࠼ࡿᙳ㡪࡟㛵ࡍࡿ◊✲
Studies on Fine Structure made from Cosmetic Baseand Its Effect on Structure of Stratum Corneumᅧ⃝ ┤⨾, ᒸ 㝯ྐ, ᒸᮏ ஽
Naomi Kunizawa, Takashi Oka, Toru Okamoto㈨⏕ᇽࣜࢧ࣮ࢳࢭࣥࢱ࣮SHISEIDO RESEARCH CENTER໬⢝ရ࡟ࡣ≉ᚩⓗ࡞ศᏊᵓ㐀ࢆ᭷ࡍࡿᇶ๣ࡀ࠶ࡾࠊ⓶⭵ࡸẟ㧥࡟㐺⏝ࡋࡓ㝿ࡢ໬⢝ရࡢຠᯝ࡟ࡶᙳ㡪ࢆཬࡰࡋ࡚࠸ࡿࠋࡇࢀࡲ࡛࡟ࡶ໬⢝ရᡂศࡀゅᒙᵓ㐀࡟ཬࡰࡍᙳ㡪࡟㛵ࡋ࡚ࡣከࡃ᳨ウࡉࢀ࡚࠸ࡿࡀࠊᇶ๣ࡢᚤ⣽࡞ศᏊᵓ㐀ࡀゅᒙᵓ㐀࡟ཬࡰࡍᙳ㡪ࡸࡑࡢ࣓࢝ࢽࢬ࣒࡟ࡘ࠸࡚ࡣ࡯࡜ࢇ࡝▱ぢࡀ࡞࠸ࠋࡑࡇ࡛ࠊ໬⢝ရᇶ๣ࢆゅᒙ࡟㐺⏝ࡋࡓ㝿ࡢゅᒙᵓ㐀ࡢኚ໬ࢆ ᐃࡋࡓ⤖ᯝࠊᇶ๣ࡢ㐪࠸࡟ࡼࡾࠊ␗࡞ࡿᵓ㐀ኚ໬ࢆほᐹࡍࡿࡇ࡜ࡀ࡛ࡁࡓࠋ࣮࣮࢟࣡ࢻ㸸 ゅᒙࠊ໬⢝ရᇶ๣ࠊ㧗ศᏊࠊ࢚ࢱࣀ࣮ࣝࠊゅᒙ⣽⬊ࠊゅᒙ⣽⬊㛫⬡㉁⫼ᬒ࡜◊✲┠ⓗ㸸໬⢝ရ࡟ࡣ⏝㏵࡟ᛂࡌ࡚ᵝࠎ࡞ᇶ๣ࡀ⏝࠸ࡽࢀ࡚࠾ࡾࠊĮ ࢤࣝࡸ࣓ࣛࣛᾮᬗ࡞࡝≉ᚩⓗ࡞ศᏊᵓ㐀యࢆ᭷ࡍࡿᇶ๣ࡶ࠶ࡿࠋࡇࢀࡽࡢศᏊᵓ㐀యࡣࠊᇶ๣ࡢᏳᐃᛶ࡟ᐤ୚ࡍࡿ࡜ྠ᫬࡟ࠊ⓶⭵ࡸẟ㧥࡟㐺⏝ࡋࡓ㝿ࡢ໬⢝ရࡢຠᯝ࡟ࡶᙳ㡪ࢆཬࡰࡋ࡚࠸ࡿࠋᚑࡗ࡚ࠊ㏆ᖺ࡛ࡣ໬⢝ရཎᩱࡸᇶ๣ࠊᡈ࠸ࡣ⓶⭵ࡸẟ㧥࡟ࡘ࠸࡚ࠊࢼࣀ࣮࢜ࢲ࣮࡟࠾ࡅࡿᵓ㐀ゎᯒ࡟㛵ࡍࡿ◊✲ࡀ┒ࢇ࡟⾜ࢃࢀ࡚࠸ࡿࠋ᭱㏆ࠊᡃࠎࡢ◊✲ࢢ࣮ࣝࣉ࡛ࡣࠊࠕ໬⢝ရᇶ๣ࠖ࡜ࡑࢀࡽࢆ⓶⭵࡟㐺⏝ࡋࡓ㝿࡟᭱ึ࡟᥋ゐࡍࡿࠕゅᒙࠖ࡟╔┠ࡋࠊ໬⢝ရᇶ๣ࡀゅᒙᵓ㐀࡟୚࠼ࡿᙳ㡪࡟㛵ࡋ࡚ྛ✀ ᐃᡭἲࢆ⏝࠸࡚◊✲ࡋ࡚ࡁࡓࠋࡇࢀࡲ࡛࡟᳨ウࡋࡓ⇕ศᯒࡲࡓࡣ ESR ࡟ࡼࡿ ᐃ⤖ᯝ࠿ࡽࠊ໬⢝ရ࡛⏝࠸ࡽࢀࡿ≉ᐃࡢᡂศࡢ㐺⏝࡟ࡼࡾゅᒙᵓ㐀ࡀኚ໬ࡍࡿࡇ࡜ࢆ☜ㄆࡋࡓࡀࠊࡇࢀࡽࡢᡂศࢆ㓄ྜࡋࡓ໬⢝ရᇶ๣୰࡛ᙧᡂࡍࡿศᏊᵓ㐀యࡀゅᒙᵓ㐀࡟ཬࡰࡍᙳ㡪࡟ࡘ࠸࡚ࡣ᳨ウ࡛ࡁ࡞࠿ࡗࡓࠋࡑࡇ࡛ࠊSPring-8 ࡟࠾ࡅࡿ㧗㍤ᗘ X ⥺ᩓ஘ ᐃ࡟ࡼࡾ໬⢝ရᇶ๣ࡀࡘࡃࡿᚤ⣽ᵓ㐀࡜ࡇࢀࡽࢆ㐺⏝ࡋࡓ㝿ࡢゅᒙᵓ㐀ࡢኚ໬࡟㛵ࡍࡿ▱ぢࢆᚓ࡚ࠊ໬⢝ရᇶ๣ࡢ⓶⭵࡟ᑐࡍࡿస⏝࣓࢝ࢽࢬ࣒ࡢヲ⣽ࢆ⪃ᐹࡋࡓࠋࡇࢀࡲ࡛࡟ࠊ໬⢝ᩱ࡟⏝࠸ࡽࢀࡿ௦⾲ⓗ࡞ᡂศࡀゅᒙ࡟ཬࡰࡍᙳ㡪࡟ࡘ࠸࡚ࡣࠊ࠸ࡃࡘ࠿ࡢ◊✲ࡀሗ࿌ࡉࢀ࡚࠸ࡿࡀࠊ໬⢝ရᇶ๣ࡀࡘࡃࡿᚤ⣽ᵓ㐀యࡀ
ゅᒙᵓ㐀࡟୚࠼ࡿᙳ㡪࡟㛵ࡍࡿሗ࿌౛ࡣ↓ࡃࠊ᪂ࡋ࠸ヨࡳ࡛࠶ࡿࠋᐇ㦂㸸໬⢝ရᇶ๣ࢆゅᒙ࡟㐺⏝ࡍࡿ๓ࠊ㐺⏝┤ᚋ࠿ࡽࡢゅᒙᵓ㐀ࡢ⤒᫬ኚ໬ࢆほᐹࡋࡓࠋලయⓗ࡟ࡣࠊX⥺ᅇᢡᐇ㦂࡟ࡣἼ㛗 0.08 nm 㻔15 keV㻕ࠊ࣓࢝ࣛ㛗 500 mmࠊ300 mm×300 mm ࡢ࢖࣓࣮ࢪࣥࢢࣉ࣮ࣞࢺࢆ⏝࠸ࡓࠋゅᒙࡣゅᒙ⣽⬊࡜ࡑࡢ࿘ࡾࢆྲྀࡾᕳࡃ⣽⬊㛫⬡㉁࠿ࡽ࡞ࡾࠊᑠゅ㡿ᇦ࡛ࡣ⣽⬊࠿ࡽࡢᩓ஘࡜᱁Ꮚᐃᩘ d ࡀ⣙ 13 nm ࡢ⬡㉁ࡢ࣓ࣛࣛᵓ㐀ࡢᅇᢡࣆ࣮ࢡࡀᚓࡽࢀࡿࠋᗈゅ㡿ᇦ࡛ࡣゅᒙ⣽⬊ࡢ୺ᡂศ࡛࠶ࡿࢣࣛࢳࣥ㻔d = 0.25-0.50 nm㻕ࡢࣈ࣮ࣟࢻ࡞ᩓ஘ୖ࡟⬡㉁ࡢ඘ሸᵓ㐀භ᪉ᬗ㻔d = 0.42 nm㻕ࡢᅇᢡࣆ࣮ࢡࡀᚓࡽࢀࡿࠋゅᒙヨᩱࡣୖୗࢆ㛤ᨺࡋࡓࢭࣝ࡟ワࡵ࡚ࠊྛ✀໬⢝ရᇶ๣ࢆ㐺⏝ࡋࠊゅᒙ࡟໬⢝ရ
ᇶ๣ࡀᾐ㏱࣭஝⇱ࡋ࡚࠸ࡃ⤒᫬ኚ໬ࢆ 3 ศẖ࡟ 30 ศᚋࡲ࡛㐃⥆ⓗ࡟ィ ࡋࡓࠋ⤖ᯝ࠾ࡼࡧ⪃ᐹ㸸
Fig. 1 ࡟Ỉࢆ㐺⏝ࡋࡓゅᒙࡢ X ⥺ᅇᢡࢹ࣮ࢱࢆ♧ࡍ㻔㻔a㻕㸸ᑠゅ㡿ᇦࠊ㻔b㻕㸸ᗈゅ㡿ᇦ㻕ࠋྛࢢࣛࣇࡣᶓ㍈࡟ S㻔nm-1㻕㻔S = 1/d㻕ࠊ⦪㍈࡟ X ⥺ᙉᗘࢆ⾲♧ࡋࠊ⤒᫬࡛ኚ໬ࡍࡿ X ⥺ᅇᢡࢹ࣮ࢱࢆ㉥Ⰽ࠿ࡽ㟷Ⰽ࡟ࢢࣛࢹ࣮ࢩ࡛ࣙࣥⰍศࡅࡋ࡚㔜ࡡ࡚⾲♧ࡋࡓࠋỈࢆ㐺⏝ࡋࡓሙྜࠊᑠゅ㡿ᇦ࡛ࡣ⤒᫬࡛ᩓ஘ᙉᗘࡀቑຍࡋࠊᗈゅ㡿ᇦ࡛ࡣỈࢆ㐺⏝┤ᚋ࡟ S = 3.2 nm-1 ௜㏆ࢆࣆ࣮ࢡ࡜ࡍࡿᩓ஘ࡀቑ࠼ࠊ⤒᫬࡛ࡑࡢࣆ࣮ࢡࡀῶᑡࡋࡓࠋỈࡣ S = 3.57 nm-1 ࡟ࣆ࣮ࢡࢆᣢࡘᩓ஘ࢆ⏕ࡌࡿࡀࠊゅᒙ࡟྾཰ࡉࢀ࡚࠸ࡿỈࡣ S = 3.2 nm-1 ௜㏆࡟ᩓ஘ࢆ⏕ࡌࡿࡇ࡜࠿ࡽࠊỈ࡜ゅᒙ࡜ࡢ┦஫స⏝ࢆ཯ᫎࡋ࡚࠸ࡿྍ⬟ᛶࡶ࠶ࡿࠋࡲࡓࠊᑠゅ㡿ᇦ࡛ቑຍࡍࡿᩓ஘ࡣ኱ࡁ࡞ᵓ㐀యࡢኚ໬ࢆ཯ᫎࡋ࡚࠸ࡿ࡜ࡍࡿ࡜ࠊゅᒙ⣽⬊ࡢ኱ࡁࡉࡢኚ໬࡟ࡼࡿ࡜⪃࠼ࡽࢀࡿࠋ
Fig. 2 ࡟≉ᐃࡢ㧗ศᏊࢆ㓄ྜࡋࡓᇶ๣ࢆ㐺⏝ࡋࡓࢹ࣮ࢱࢆ♧ࡍࠋ㧗ศᏊࢆ㓄ྜࡋࡓᇶ๣࡛ࡣᑠゅ㡿ᇦ࡟ࡣ࡯࡜ࢇ࡝ኚ໬ࡀ࡞ࡃࠊゅᒙ⣽⬊ࡢ኱ࡁࡉࡢኚ໬ࡣᑡ࡞࠸࡜⪃࠼ࡽࢀࡿࠋᗈゅ㡿ᇦ࡛ࡣࠊS = 3.2
nm-1 ௜㏆ࡢᩓ஘ࡀỈࡼࡾࡶ㐜ࡃࠊỈศࡢ⵨Ⓨࡀ㐜࠸ࡇ࡜ࡀⰋࡃศ࠿ࡿࠋࡉࡽ࡟ࠊ࢚ࢱࣀ࣮ࣝࢆ㓄ྜࡋࡓᇶ๣࡛ࡣᑠゅ㡿ᇦ࡛ࡣỈ࡜ࡢᕪࡣ࠶ࡲࡾ࡞࠿ࡗࡓࡀࠊᗈゅ㡿ᇦ࡛ࡣS = 3.2 nm-1 ௜㏆ࡢᩓ஘ࡢῶᑡࡀ᪩ࡃࠊỈࡢῶᑡࡀ᪩࠸ࡇ࡜ࢆ♧၀ࡋ࡚࠸ࡿࠋࡇࢀࡲ࡛ࡢ᳨ウ࡛ࡣࠊᇶ๣ࡢࡳࡢᵓ㐀ゎᯒࡸࠊ኱㔞ࡢ⁐๣ࢆ㐺⏝ࡋ࡚⁐๣ࢆ྾཰ࡋ࡚࠸ࡃ㐣⛬࣭⁐๣ࢆ༑ศ྾཰ࡉࡏࡓゅᒙࡀ஝⇱ࡋ࡚࠸ࡃ㐣⛬ࢆࡑࢀࡒࢀィ ࡋ࡚࠸ࡓ[1-4]ࠋ௒ᅇࡣࠊᇶ๣ࢆ㐺⏝ࡋ࡚࠿ࡽ྾཰࣭஝⇱ࡀ⏕ࡌࡿ㐣⛬ࢆࡑࡢࡲࡲィ ࡍࡿࡇ࡜࡟ᣮᡓࡋࡓࠋࡑࡢ⤖ᯝࠊ྾཰ࡀ㐜࠸ᇶ๣ࡸ஝⇱ࡀ㏿࠸ᇶ๣࡟ࡼࡗ࡚ࠊᑠゅ㡿ᇦ࣭ᗈゅ㡿ᇦ࡛ࡢᣲືࡀ␗࡞ࡿࡇ࡜ࡀศ࠿ࡾࠊ໬⢝ရࢆ⫙࡟ሬᕸࡋࡓ᫬ࡢ≧ἣࢆ෌⌧ࡍࡿᐇ㦂࡜ࡋ࡚ࠊ㠀ᖖ࡟㔜せ࡞▱ぢࢆᚓࡿࡇ࡜ࡀ࡛ࡁࡓࠋࡲࡓࠊ௒ᅇࡢ᳨ウࡔࡅ࡛ࡣࡑ
ࢀࡒࢀࡢᵓ㐀యࢆ≉ᐃࡍࡿࡇ࡜ࡣ࡛ࡁ࡞࠸ࡀࠊస⏝ࡉࡏࡿᇶ๣ࡢ㐪࠸࡟ࡼࡾኚ໬ࡍࡿゅᒙࡢᵓ㐀యࡀ␗࡞ࡿ࡜࠸࠺㔜せ࡞⤖ᯝࢆᚓࡿࡇ࡜ࡀ࡛ࡁࡓࠋ

㻔a㻕

㻔b㻕

Fig. 1 The X-ray diffraction patterns of stratum corneum samples treated with water during absorption
and evaporation of water. The small angle 㻔a㻕 and the wide angle 㻔b㻕 diffraction patterns.
The change in 0-to-30 minutes is shown by red-to-blue.

㻔a㻕

㻔b㻕

Fig. 2 The X-ray diffraction patterns of stratum corneum samples treated with cosmetic base contained
a polymer during absorption and evaporation of water. The small angle 㻔a㻕 and the wide angle
㻔b㻕 diffraction patterns. The change in 0-to-30 minutes is shown by red-to-blue.

௒ᚋࡢㄢ㢟㸸௒ᅇࡣࠊゅᒙ࡟㐺⏝ࡋࡸࡍ࠸⁐ᾮ⣔ࡢᇶ๣ࢆ⏝࠸ࡓࡀࠊ௒ᚋࡣࡼࡾᇶ๣ࡀ≉ᚩⓗ࡞ᚤ⣽ᵓ㐀ࢆᣢࡘ⢓ᗘࡢ㧗࠸ᇶ๣࡛ࡢ㐺⏝᪉ἲࢆ⪃᱌ࡋࠊᚤ⣽ᵓ㐀ࡀゅᒙᵓ㐀࡟୚࠼ࡿᙳ㡪࡟ࡘ࠸࡚ࡼࡾヲ⣽࡞᳨ウࢆ
⾜࠺ᚲせࡀ࠶ࡿࠋࡲࡓࠊࡑࢀࡒࢀࡢᵓ㐀యࡢኚ໬࡜ᇶ๣ࡢస⏝࣓࢝ࢽࢬ࣒࡟ࡘ࠸࡚ࡼࡾヲࡋ࠸᳨ウࢆ⾜࠸ࡓ࠸ࠋཧ⪃ᩥ⊩㸸
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