Information of Training Course
All the activities will be performed at the Research Center for Zoonosis Control, Hokkaido University.
DatethSep. 18 (Wed)Sep. 19th (Thu)

TimeFree time
9:00-Activity

Arrival day1) Instructions relating to travel expense procedure(GI-CoRE staff)
13:002) General Orientation
13:303) Practice for setting traps for mosquito sampling(Tutor/Teacher: Drs. Yuki Eshita, Yasuko Orba)th
Sep. 20 (Fri)9:301) Presentation of the situation of research activities in each country by eachparticipant (Country Report: each 10 min)(Tutor/Teacher: Drs. Yasuko Orba, Hirofumi Sawa)
2) Practice for identification of collected mosquitoes(Tutor/Teacher: Drs. Yuki Eshita, Yasuko Orba, Hirofumi Sawa)
stSep 21 (Sat),
WeekendFree timeSep 22nd (Sun)
Weekend or
1) Free time or Practice for ticks sampling in the field around Lake Shikotsu
Option (tickhttps://www.japan-guide.com/e/e6736.html
sampling)(Tutor/Teacher: Drs. Stephen Baker, Dayana Baker, Shiho Torii, Yongjin Qiu,Keita Matsuno, Yasuko Oba, Ryo Nakao, Hirofumi Sawa)
rdSep 23 (Mon)13:301) Practice for identification of ticks
Japanese National(Tutor/Teacher: Drs. Yongjin Qiu, Ryo Nakao, Stephen Barker, Hirofumi Sawa)
Holiday
Sep 24th (Tue)9:00 1) Isolation of RNAs from collected mosquitoes and ticks2) RT-PCR for flavivirus (for MinION experiment) & phlebovirus (for Sangersequencing)(Tutor/Teacher: Drs. Keita Matsuno, Yasuko Orba, Hirofumi Sawa,William Hall)Sep 25th (Wed)9:001) Gel electrophoresis of RT-PCR products2) Extraction of phlebovirus RT-PCR products from agarose gel3) Extraction of flavivirus RT-PCR products using SPRI beads4) MinION experiment 1 using purified RT-PCR products: dA tailing(Tutor/Teacher: Drs. Kyoko Hayashida, Hirofumi Sawa, William Hall, Teachingassistant: Dr. Walter Muleya)Sep 26th (Thu)
9:001) MinION experiments 2: whole day experiments (ligation of adapters, clean up,and so on)(Tutor/Teacher: Ms.Naoko Kawai, Drs. Kyoko Hayashida, Junya Yamagishi,Hirofumi Sawa, William Hall, Teaching assistant: Dr. Walter Muleya )Sep 27th (Fri)9:001) Bioinformatics practice I: Basic Linux command line
(Tutor/Teacher: Drs. Gabriel Gonzalez, Junya Yamagishi, Hirofumi Sawa,William Hall)
2) Data analysis of results by MinION analysis (demonstration)(Tutor/Teacher: Drs. Gabriel Gonzalez, Junya Yamagishi, Hirofumi Sawa,William Hall, Teaching assistant: Dr. Walter Muleya)Sep 28th (Sat)9:001) Data analysis of results by MinION analysis by trainee (part I)(Tutor/Teacher: Drs. Junya Yamagishi, Kyoko Hayashida, William Hall)th
Sep 29 (Sun)
WeekendFree timeSep 30th (Mon)9:001) Sanger sequencing of phlebovirus RT-PCR products from agarose gel(Tutor/Teacher: Ms.Yumi Saito, Drs. Yasuko Orba, Hirofumi Sawa,William Hall, Teaching assistant: Dr. Mariko Tanaka)
2) Phylogenetic analysis using data from MinION and sanger sequencing(Tutor/Teacher: Drs. Gabriel Gonzalez, Hirofumi Sawa, William Hall
Teaching assistant: Dr. Mariko Tanaka)
Oct 1st (Tue)9:001) Data analysis of results by MinION analysis by trainee (part II)(Tutor/Teacher: Drs. Junya Yamagishi, William Hall,Teaching assistant: Dr. Mariko Tanaka)
18:002) Farewell DinnerOct 2nd (Wed)9:001) Useful tools in bioinformatics(Tutor/Teacher: Drs. Gabriel Gonzalez, Hirofumi Sawa, William Hall
Teaching assistant: Dr. Mariko Tanaka)
2) Site tour
(Tutor/Teacher: Drs. Gabriel Gonzalez, Yuki Eshita, Hirofumi Sawa,William Hall, Teaching assistant: Dr. Mariko Tanaka)