Enabling Epigenetics Research

MicroRNAs&HUMAN DISEASE
publications highlighting current research identifying
the associations between miRNAs and diseaseEnabling Epigenetics Research

MicroRNAs& Human Disease
MicroRNAs (miRNAs) are a class of small non-coding RNAs which normally function as post-transcriptional regulators thatinhibit target mRNA expression. miRNAs associate with the 3´UTR of target mRNAs where they induce cleavage ortranslational inhibition of target mRNAs. There has been a growing increase in reports associating miRNAs with humandisease. However, the regulatory networks governing miRNA-disease associations remain largely unclear. In order to further
our understanding of the associations between miRNAs and human disease, innovative tools are needed to identify novel
disease-associated microRNAs and to study their regulatory functions.
Active Motif’s LightSwitch™ 3´UTR and miRNA products are ideal for performing miRNA target validation, as well as assessing the functional impact of miRNA-3´UTR interactions. These include a collection of over 12,000 3´UTR constructs, miRNA
Mimics and Inhibitors, and over 900 optimized LightSwitch Synthetic miRNA Target Reporter constructs to ensure you haveeverything needed to validate miRNA targets, measure RNA stability, translation efficiency and the functional impact of miRNAs.
To also aid in the introduction and understanding of miRNA-disease associations, this reference piece presents a selectionof recently published articles featuring the use of LightSwitch products for disease research. For more on the LightSwitch
Luciferase Assay System and products, please visit us at www.activemotif.com/lightswitch.

ONCOLOGY
Agrawal, P. et al. (2014) Mapping posttranscriptional regulation of the human glycome uncovers microRNA defining the glycocode. Proc. Natl. Acad. Sci. U. S. A. 111, 4338–43.
Gururajan, M. et al. (2014) miR-154* and miR-379 in the DLK1-DIO3 microRNA mega-cluster regulate epithelial to mesenchymal transition and bone metastasis of prostate cancer.
Clin. Cancer Res. 20, 6559–69.
Hamilton, M. P. et al. (2013) Identification of a pan-cancer oncogenic microRNA superfamily anchored by a central core seed motif. Nat. Commun. 4, 2730.
Selcuklu, S. D. et al. (2012) MicroRNA-9 inhibition of cell proliferation and identification of novel miR-9 targets by transcriptome profiling in breast cancer cells. J. Biol. Chem. 287,29516–28.
Tang, X. et al. (2014) Glycogen synthase kinase 3 beta inhibits microRNA-183-96-182 cluster via the β-Catenin/TCF/LEF-1 pathway in gastric cancer cells. Nucleic Acids Res. 42,2988–98.

OBESITY AND DIABETES
Cifarelli, V. et al. (2015) Metformin and Rapamycin Reduce Pancreatic Cancer Growth in Obese Prediabetic Mice by Distinct MicroRNA-Regulated Mechanisms. Diabetes.
doi:10.2337/db14-1132.
Clarke-Harris, R. et al. (2014) PGC1α promoter methylation in blood at 5-7 years predicts adiposity from 9 to 14 years (EarlyBird 50). Diabetes. 63, 2528–37.
Jenkins, Y. et al. (2013) AMPK activation through mitochondrial regulation results in increased substrate oxidation and improved metabolic parameters in models of diabetes. PLoS
One. 8, e81870.
Ramkhelawon, B. et al. (2014) Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat. Med. 20, 377–84.
Yang, M. et al. (2014) Identification of miR-185 as a regulator of de novo cholesterol biosynthesis and low density lipoprotein uptake. J. Lipid Res. 55, 226–38.

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Enabling Epigenetics Research

IMMUNOLOGY AND INFLAMMATION
Kelly, C. J. et al. (2013) Fundamental role for HIF-1α in constitutive expression of human β defensin-1. Mucosal Immunol. 6, 1110–8.
Khosravi, R. et al. (2014) A novel function for lysyl oxidase in pluripotent mesenchymal cell proliferation and relevance to inflammation-associated osteopenia. PLoS One. 9, e100669.
Lill, C. M. et al. (2014) Assessment of microRNA-related SNP effects in the 3’ untranslated region of the IL22RA2 risk locus in multiple sclerosis. Neurogenetics. 15, 129–34.
Pham, D. et al. (2014) The transcription factor Etv5 controls TH17 cell development and allergic airway inflammation. J. Allergy Clin. Immunol. 134, 204–14.
Xu, N. et al. (2013) MicroRNA-31 is overexpressed in psoriasis and modulates inflammatory cytokine and chemokine production in keratinocytes via targeting serine/threonine
kinase 40. J. Immunol. 190, 678–88.

STEM CELLSChao, C.-H. et al. (2014) MicroRNA-205 signaling regulates mammary stem cell fate and tumorigenesis. J. Clin. Invest. 124, 3093–106.
Dolezalova, D. et al. (2012) MicroRNAs regulate p21Waf1/Cip1 protein expression and the DNA damage response in human embryonic stem cells. Stem Cells. 30, 1362–72.
Egea, V. et al. (2012) Tissue inhibitor of metalloproteinase-1 (TIMP-1) regulates mesenchymal stem cells through let-7f microRNA and Wnt/β-catenin signaling. Proc. Natl. Acad. Sci.
U. S. A. 109, E309–16.
Fang, X. et al. (2014) The zinc finger transcription factor ZFX is required for maintaining the tumorigenic potential of glioblastoma stem cells. Stem Cells. 32, 2033–47.
Karlsen, T. A. et al. (2014) MicroRNA-140 targets RALA and regulates chondrogenic differentiation of human mesenchymal stem cells by translational enhancement of SOX9 andACAN. Stem Cells Dev. 23, 290–304.

NEUROBIOLOGY
Cavaliere, F. et al. (2013) NMDA modulates oligodendrocyte differentiation of subventricular zone cells through PKC activation. Front. Cell. Neurosci. 7, 261.
Hou, S. T. et al. (2013) Collapsin response mediator protein 3 deacetylates histone H4 to mediate nuclear condensation and neuronal death. Sci. Rep. 3, 1350.
Morgado, A. L. et al. (2014) MicroRNA-34a modulates neural stem cell differentiation by regulating expression of synaptic and autophagic proteins. Mol. Neurobiol.
doi:10.1007/s12035-014-8794-6.
Schröder, J. et al. (2014) MicroRNA-138 is a potential regulator of memory performance in humans. Front. Hum. Neurosci. 8, 501.
Vincelli, G. et al. (2015) Renilla luciferase reporter assay to study 3’UTR-driven posttranscriptional regulations of OPRM1. Methods Mol. Biol. 1230, 53–63.

CARDIOLOGY & CARDIOVASCULAR MEDICINEChiang, D. Y. et al. (2015) Identification of microRNA-mRNA dysregulations in paroxysmal atrial fibrillation. Int. J. Cardiol. 184C, 190–97.
Danielson, L. S. et al. (2013) Cardiovascular dysregulation of miR-17-92 causes a lethal hypertrophic cardiomyopathy and arrhythmogenesis. FASEB J. 27, 1460–67.
Kojima, Y. et al. (2014) Cyclin-dependent kinase inhibitor 2B regulates efferocytosis and atherosclerosis. J. Clin. Invest. 124, 1083–97.
Maegdefessel, L. et al. (2014) miR-24 limits aortic vascular inflammation and murine abdominal aneurysm development. Nat. Commun. 5, 5214.
Varga, Z. V. et al. (2013) MicroRNA-25-dependent up-regulation of NADPH oxidase 4 (NOX4) mediates hypercholesterolemia-induced oxidative/nitrative stress and subsequentdysfunction in the heart. J. Mol. Cell. Cardiol. 62, 111–21.

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EUROPE +32 (0)2 653 0001

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www.activemotif.com

Enabling Epigenetics Research

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