Osami Shoji, Prof.
Affiliation: Department of Chemistry, Graduate School of Science,Nagoya University
Address: Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan
Tel: +81-52-789-2955
E-mail: shoji.osami@a.mbox.nagoya-u.ac.jp
Homepage: http://bioinorg.chem.nagoya-u.ac.jp/
Professional Career2019-present
Professor at Graduate School of Science, Nagoya University
2013–2019
Associate Professor at Graduate School of Science, Nagoya University
2008–2013
Assistant Professor at Graduate School of Science, Nagoya University
2007–2008
Assistant Professor at Research Center for Materials Science, Nagoya University
2006–2007
JSPS Postdoctoral fellow (SPD)2005–2006
Postdoctoral fellow at Graduate School of Science, Nagoya University
2004–2005
Postdoctoral fellow at Graduate School of Materials Science, Nara Institute ofScience and Technology
2002–2004
Postdoctoral fellow of the Core Research for Evolutional Science and Technology(CREST) project of Japan Science and Technology Agency (JST)
Education
1999–2002
Graduate School of Science and Technology, Chiba University
Received a PhD (Prof. Takayuki Nakahira)
1997–1999
Graduate School of Science and Technology, Chiba University
1993–1997
Department of Applied Chemistry, Faculty of Engineering, Chiba University
Selected Publications[1]

O. Shoji, Y. Aiba, Y. Watanabe, "Hoodwinking Cytochrome P450BM3 into Hydroxylating Non-Native Substrates byExploiting Its Substrate Misrecognition" Acc. Chem. Res. 2019, 52, 925-934.
[2] K. Omura, Y. Aiba, H. Onoda, J. K. Stanfield, S. Ariyasu, H. Sugimoto, Y. Shiro, O. Shoji, Y. Watanabe, "Reconstitutionof full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A" Chem. Commun. 2018,54, 7892-7895.
[3] M. Karasawa, J. K. Stanfield, S. Yanagisawa, O. Shoji, Y. Watanabe, "Whole-Cell Biotransformation of Benzene toPhenol Catalysed by Intracellular Cytochrome P450BM3 Activated by External Additives" Angew. Chem. Int. Ed. 2018,57, 12264-12269.
[4] H. Uehara, Y. Shisaka, T. Nishimura, H. Sugimoto, Y. Shiro, Y. Miyake, H. Shinokubo, Y. Watanabe, O. Shoji, "Structuresof the Heme Acquisition Protein HasA with Iron(III)-5,15-Diphenylporphyrin and Derivatives Thereof as an Artificial
Prosthetic Group" Angew. Chem. Int. Ed. 2017, 56, 15279-15283.
[5] K. Suzuki, J. K. Stanfield, O. Shoji, S. Yanagisawa, H. Sugimoto, Y. Shiro, Y. Watanabe, "Control of stereoselectivity ofbenzylic hydroxylation catalysed by wild-type cytochrome P450BM3 using decoy molecules" Catal. Sci. Technol. 2017,7, 3332-3338.
[6] O. Shoji, S. Yanagisawa, J. K. Stanfield, K. Suzuki, Z. Cong, H. Sugimoto, Y. Shiro, Y. Watanabe, "Direct Hydroxylationof Benzene to Phenol by Cytochrome P450BM3 Triggered by Amino Acid Derivatives" Angew. Chem. Int. Ed. 2017, 56,10324-10329.
[7] Z. Cong, O. Shoji, C. Kasai, N. Kawakami, H. Sugimoto, Y. Shiro, Y. Watanabe, "Activation of Wild-Type Cytochrome
P450BM3 by the Next Generation of Decoy Molecules: Enhanced Hydroxylation of Gaseous Alkanes andCrystallographic Evidence" ACS Catal. 2015, 5, 150-156.
[8] C. Shirataki, O. Shoji, M. Terada, S.-i. Ozaki, H. Sugimoto, Y. Shiro, Y. Watanabe, "Inhibition of heme uptake inPseudomonas aeruginosa by its hemophore (HasA(p)) bound to synthetic metal complexes" Angew. Chem. Int. Ed. 2014,
53, 2862-2866.
[9] O. Shoji, T. Kunimatsu, N. Kawakami, Y. Watanabe, "Highly selective hydroxylation of benzene to phenol by wild-type
cytochrome P450BM3 assisted by decoy molecules" Angew. Chem. Int. Ed. 2013, 52, 6606-6610.
[10] N. Kawakami, O. Shoji, Y. Watanabe, "Direct hydroxylation of primary carbons in small alkanes by wild-type cytochrome
P450BM3 containing perfluorocarboxylic acids as decoy molecules" Chem. Sci. 2013, 4, 2344-2348.
[11] N. Kawakami, O. Shoji, Y. Watanabe, "Use of perfluorocarboxylic acids to trick cytochrome P450BM3 into initiating thehydroxylation of gaseous alkanes" Angew. Chem. Int. Ed. 2011, 50, 5315-5318.

Research Interests
1. Alteration of Substrate Specificity of Cytochrome P450s
2. Gaseous Alkane Hydroxylation by Cytochrome P450s
3. Engineering of Heme Acquisition Proteins