Endocrine Disorders

Reagents for Assay Development
ISO Certified

www.meridianlifescience.com

Company Overview

Extensive Capabilities & Services
Molecular Reagents
qPCR | RT-qPCR | LAMPENZYMES

Immuno Reagents
Antigens | Antibodies | Blockers•	Hot-Start Taq technologies - chemical,
antibody, aptamer

VIRUS MANUFACTURING•	Lyo & Air-Dryable enzymes (glycerol free) Taq,
Bst, RTase•	Proprietary Ag purification•	Live or inactivated•	Thermostable MMLV RT

RECOMBINANT PROTEINSMASTER MIXES•	Lyo & Air-Dryable formats•	Inhibitor-tolerant mixes for stool, sputum,
saliva, blood, plant, water.
•	For multiplexing, GC-rich templates•	
E. coli, P. pastoris, S. cerevisiae, Sf9,
Mammalian (CHO, HEK293)•	10L- 130L fermentation

ANTIBODIES – MAbs/PAbs•	500+ MAbs produced in grams

II

NUCLEOTIDES•	Multi-Kilograms of MIgG / year•	dNTPs, Na or Li salts•	Hundreds of liters of GxhIgG•	Ultra high purity, >99%•	Ascites production (55,000 Mice)

Endocrine Disorders - Reagents for Assay Development

Commercial scale manufacturingof antigens and antibodies withprotein purification expertise.
Meridian has been providing innovative life science solutions and building
trusted partnerships for over 43 years. Meridian’s focus is to offer complete
solutions for the development of molecular and immunological assays.
• Full line of immunoassay reagents, including antigens, antibodies and blockers• Large scale production of reagents for molecular assays• Technical support with assay development experience• Dedicated R&D and manufacturing teams•R obust and mature Quality System

ISO Certified 13485:2016

Global presence
WATERLOO, Belgium
QUEBEC, Canada

LONDON, United Kingdom
LUCKENWALDE, Germany

BILLERICA, MA
MANASQUAN, NJ
CINCINNATI, OH (Headquarters)

BEIJING, China

PARIS, France

CHANGZHOU, China

MILAN, Italy
MEMPHIS, TN
BOCA RATON, FL

MODIIN, Israel

SYDNEY, Australia

Diagnostic Manufacturing |Life Science Manufacturing |Sales & Warehouse

MERIDIAN BIOSCIENCE, INC.
Parent Company | Founded in 1977 | Nasdaq: VIVO | 750+ Employees
Headquartered in Cincinnati, OH | Presence in 70+ Countries.

www.meridianlifescience.com

IIICompany Overview
Antigens & Antibodies
INFECTIOUS DISEASE EXPERTISE

IV

Tropical

ToRCH & Childhood

Viral Hepatitis•	Zika•	Dengue 1, 2, 3, 4
•	Chikungunya•	Malaria•	Chagas•	Leishmaniasis•	Leptospirosis•	Newcastle Disease•	Yellow Fever•	Nipah Virus•	JEV•	Toxo•	Rubella•	CMV•	HSV-1,2•	Rubeola•	EBV•	Mumps•	Coxsackie•	Rotavirus•	RSV•	Parvo B19•	VZV•	HAV•	HBV•	HCV•	HDV•	HEV

Endocrine Disorders - Reagents for Assay Development

STD•	HSV-1, 2•	HIV-1, 2•	HPV•	Syphilis•	Chlamydia•	Neisseria

Gastro•	
H. Pylori•	C. Difficile•	Norovirus•	Adenovirus•	Rotavirus•	Cryptosporidium•	Campylobacter•	
E. Coli•	Salmonella•	
G. Lambia•	Astrovirus

Respiratory•	SARS-CoV-2•	
M. Pneumoniae•	
C. Pneumoniae•	Influenza A, B
•	Parainfluenza•	
L. Pneumophilia•	RSV•	
M. Tuberculosis•	Streptococcus•	Staphylococcus•	Adenovirus

Cardiac

Cancer

Veterinary

Drug of Abuse•	Troponin I, T•	Myoglobin•	BNP•	NT-proBNP•	CRP•	PCT•	CK-MB•	D-Dimer•	Cystatin-C•	Galectin-3•	Vitamin D•	Apo A, B, E
•	NSE•	FABP•	SAH•	MPO•	Fibrinogen•	EGF•	Lp-PLA2•	PAPP-A•	CA125•	CA15-3•	CA19-9•	CA72-4•	CA50•	CA242•	Cyfra 21-1
•	CEA•	Thyroglobulin•	erbB-2/HER2•	AFP•	EGFR•	HE4•	NSE•	PMA•	PAP•	PSA•	PSMA•	S-100•	PIVKA II•	B2M•	ASFV•	Avian Influenza•	Borrelia•	
Brucella abortus•	Canine Distemper•	Feline
Immunodeficiency•	Feline Leukemia•	Foot-and-Mouth•	Canine Heartworm•	Infectious Bursal
Disease•	Marek Disease•	Newcastle Disease•	Canine Parvovirus•	Rabies Virus•	Serum Amyloid A (SAA)• Trichomonas foetus•	Nipah•	Transmissible
Gastroenteritis•	Amphetamine•	Barbital•	Benzodiazepine•	Buprenorphine•	Cocaine•	Cotinine•	EDDP•	Fentanyl•	Ketamine•	K2•	MDMA (Ecstasy)•	Methadone•	Methamphetamine•	Morphine•	Norketamine•	Opium•	Oxycodone•	PCP•	Phenobarbital•	Propoxyphene•	THC

Autoimmune

Microbial Detection•	Jo-1•	PCNA•	pANCA•	cANCA•	Sm Ag•	dsDNA•	La(SSA)•	Ro(SSA)•	Histone•	GMB•	C1q•	Scl-70•	SS-A•	BS-Gly-1•	Cathepsin G•	Calprotectin•	Legionella•	Salmonella•	Cryptosporidium•	
G. Lambia•	
C. Jejuni•	
E. Coli•	
B. Anthracis•	Clostridium•	Listeria•	Streptococcus•	Staphylococcus

Immunoglobulins/
Blockers

Hormones•	LH, FSH, hCG,
•	hGH, AMH•	Cortisol•	Estradiol•	Insulin, C-peptide•	Prolactin•	Progesterone•	PTH•	PAPP-A•	TSH, T3, T4, ACTH
•	Thyroglobulin

Allergens•	Cat & Dog Allergen•	Horse Allergen•	Dust Mite
• Alternaria alternate•	Timothy Grass•	
Platanus acerifolia•	Mugwort•	TRU Block™ &IgM Diluent•	Animal IgGs – Bovine,
Chicken, Goat, Mouse,
Rabbit, Sheep•	Human IgA, IgG,
IgM, IgE• Kappa Light chain• Lambda Light chain•	Goat Anti-Human
IgG, IgM, IgA•	Goat Anti-Mouse IgG

www.meridianlifescience.com

VEndocrine Disorders
The endocrine system is a network of glands that produce and releasehormones which regulate mood, growth and development, tissue function,
metabolism, as well as sexual function and reproductive processes.
The eight major glands that make up the humanendocrine system are the hypothalamus, pancreas,
pituitary, thyroid, parathyroids, adrenals, pineal body,and the reproductive glands, (which include the ovaries
and testes). The pancreas, which is mainly associatedwith the digestive system, is also part of this hormonesecreting system. Overall, although the endocrine
glands are the body’s main hormone producers, some
non-endocrine organs — such as the brain, heart,lungs, kidneys, liver, thymus, skin, and placenta— also produce and release hormones.
Endocrine disorders are diseases that specificallyrelate to the endocrine glands and they are generallygrouped into two categories:• Disease that results from producing too much or
too little hormone, leading to a hormone imbalance• Disease that results from the development of a lesion(such as a nodule or tumor) in the endocrine system
which may or may not affect hormone levels
Common endocrine disorders include diabetes,
acromegaly (overproduction of growth hormone),
Addison’s disease (decreased production of hormonesby the adrenal glands), Cushing’s syndrome (highcortisol levels for extended periods of time),
hyperthyroidism (overactive thyroid), hypothyroidism(underactive thyroid), and prolactinoma (overproductionof prolactin by the pituitary gland). These disorders often
have widespread symptoms, affect multiple parts of thebody, and can range from mild to very severe. Treatments
depend on the specific disorder but often requires theuse of synthetic hormones.
Diagnosis for endocrine disorders is usually made using
blood, urine or saliva tests that measure hormone levels.
There is no single ideal method for assessment as eachhave their advantages and disadvantages. Serum-based
assays provide a direct measurement of circulating
hormones but are generally unable to distinguish theprotein-bound, inactive form of the hormone from its freeand biologically active form. Serum testing is ideal for
peptide hormones such as FSH, LH, prolactin, fasting
insulin, and thyroid hormones, including reverse T3, as
well as thyroid antibodies. Serum tests can also be used tomeasure sex hormone binding globulin (SHBG) and, lesscommonly, cortisol binding globulin (CBG).
In contrast, urine assays measure unbound hormone,reflecting the bioavailable levels. A 24-hour urine collection is
the preferred method for assessing physiological hormone
levels because it provides a comprehensive picture as
opposed to a single time point analysis. Saliva testing has
also gained in popularity and has the advantage of being
noninvasive as well as being accessible to practitioners suchas chiropractors, and acupuncturists who may be practicing
in regions where they are not licensed to order blood tests
or draw blood. Saliva collection also allows for multiple
collections over a period of a day or month, which canhelp elucidate abnormal hormonal patterns, such as ashortened luteal phase.
New diagnostic testing looks at the genetic basis for theendocrine disease. A variety of endocrine disorders arecaused by gene variations which are now well understoodin terms of their molecular basis and mode of inheritance.
The recent advances in molecular testing and genomics
have uncovered that genes play a far more important rolein the pathogenesis of endocrine disease than previously
appreciated. Overall, through the earlier detection ofgenetic carriers and/or through diagnosing the exact
subtype of the disease, earlier and more targetedintervention and treatments are possible.

1

Endocrine Disorders - Reagents for Assay Development

ENDOCRINE GLANDS& THEIR HORMONES
THYROID & PARATHYROIDGLANDPITUITARY GLAND &HYPOTHALAMUS•	Parathyroid hormone (PTH)•	Thyroglobulin (Tg)•	Thyroid peroxidase (TPO)•	Thyroid-stimulating
hormone (TSH)•	Thyroxine-binding
globulin (TBG)•	Thyroxine (T4)•	Triiodothyronine (T3)•	Adrenocorticotropic
hormone (ACTH)•	Human growth
hormone (hGH)•	Luteinizing hormone (LH)•	Prolactin•	Thyroid-stimulating
hormone (TSH)
OVARIES & TESTESADRENAL GLANDS•	Anti-Müllerian
hormone (AMH)•	Dehydroepiandrosterone(DHEA)•	Estradiol (E2)•	Estriol (E3)•	Follicle-stimulating
hormone (FSH)•	Human chorionicgonadotropin (hCG)•	Luteinizing hormone (LH)•	Pregnancy-associatedplasma protein-A (PAPP-A)•	Progesterone (P4)•	Prolactin (PRL)•	Testosterone•	Adrenocorticotropic
hormone (ACTH)•	Aldosterone•	Cortisol
PANCREAS•	Glucagon• Insulin (C-peptide)

Catalog Guide
Company overview................................ ii
Adrenocorticotropic hormone (ACTH)...3
Aldosterone............................................4
Anti-Müllerian hormone (AMH).............5
Calcitonin................................................7
Cortisol....................................................8
Dehydroepiandrosterone (DHEA)..........9
Estradiol (E2).........................................10
Estriol (E3).............................................11
Follicle-stimulating hormone (FSH)......12

Glucagon...............................................13
Human chorionic gonadotropin (hCG)...14
Human growth hormone (hGH).............16
Insulin (C-peptide).................................17
Luteinizing hormone (LH).....................18
Parathyroid hormone (PTH)..................19
Pregnancy-associated plasma
protein-A (PAPP-A)................................20
Progesterone (P4).................................21

Prolactin (PRL)......................................22
Testosterone.........................................23
Thyroglobulin (Tg).................................25
Thyroid peroxidase (TPO).....................26
Thyroid-stimulating hormone (TSH).....27
Thyroxine-binding globulin (TBG).........28
Triiodothyronine (T3) & Thyroxine (T4)...29
Full product list.....................................30

www.meridianlifescience.com

2Adrenocorticotropic hormone (ACTH)
ACTH is a hormone secreted by the pituitary gland and is often
produced in response to biological stress. Its principal effect isincreased production and release of cortisol by the cortex of
the adrenal gland. ACTH also plays a role in circadian rhythm inmany organisms.
Deficiency of ACTH leads to a reduction in the secretion of adrenal hormones(e.g. adrenaline, aldosterone and cortisol), resulting in secondary adrenal insufficiency(hypoadrenalism), the manifestations of which are clinically indistinguishable from thoseof glucocorticoid deficiency. Symptoms include weight loss, lack of appetite, muscle
weakness, nausea and vomiting, and low blood pressure (hypotension). ACTH
deficiency can either be congenital or acquired, and several genetic mutations havebeen linked to this disease.
In contrast, chronically elevated ACTH levels occur in primary adrenal insufficiency
in which damage to the adrenal glands prevents them from producing the hormonesin adequate amounts. An example is Addison’s disease which can be caused by
autoimmune disorders or infections, such as TB or HIV, and tumors. Another disorder,
Cushing’s disease, can be caused by medication or by a pituitary tumor and leads to an
excess of cortisol (hypercortisolism).
Quantitative plasma ACTH assays are useful inthe differential diagnosis of pituitary Cushing’s
disease, Addison’s disease, autonomous ACTH
producing pituitary tumors (e.g. Nelson’s syndrome),
hypopituitarism with ACTH deficiency and ectopic ACTH
syndrome. Hypopituitarism with ACTH deficiency, which issecondary adrenocortical insufficiency, is characterized by
low plasma ACTH and cortisol concentrations, and asubnormal, but usually distinct adrenal response tostimulation with synthetic ACTH (Cortrosyn).

Reagents for Immunoassay Development
E54057M

PAIR

E01372M

3

E01373M

MAb to ACTH N-Terminal• Specific for Synacthen (1-24 ACTH)• Reacts with ACTH (a.a. 1–17) and has nocross-reactivity with CLIP (ACTH 17-39)

Suitable for use in
ELISA & IHC

MAb to ACTH N-Terminal• Capture antibody• Reacts with ACTH a.a. 1–39 and a.a. 1-24
• Minimal cross reaction (< 0.02%) with CLIP, ß-LPH,
ß-endorphin and Insulin
MAb to ACTH N-Terminal• Detection antibody• Reacts with ACTH a.a. 1–39 and a.a. 1-24
• Minimal cross reaction (< 0.02%) with CLIP,
ß-LPH, ß-endorphin and Insulin

Suitable for ELISA,WB and IHC

Endocrine Disorders - Reagents for Assay Development

Aldosterone
Aldosterone is a steroid hormone produced by the adrenal cortex
that plays an important role in cardiac health and can be a cause of
endocrine hypertension. It is essential for sodium conservation in thekidney, salivary glands, sweat glands and colon, and is involved in
the homeostatic regulation of blood pressure, plasma sodium (Na+),and potassium (K+) levels.
Aldosterone is closely linked to two other hormones, renin and angiotensin, and together
these are the renin-angiotensin-aldosterone system. This system is activated when the
body experiences a decrease in blood flow to the kidneys, such as after a drop in bloodpressure, or a significant drop in blood volume after a hemorrhage or serious injury.
Primary aldosteronism (Conn syndrome) is caused by the overproduction of aldosteroneby the adrenal glands, usually from a benign tumor or a genetic disorder (familial
hyperaldosteronism). The high aldosterone level increases reabsorption of sodium andloss of potassium by the kidneys, often resulting in an electrolyte imbalance. Secondary
aldosteronism, which is more common than primary aldosteronism, is caused by anythingthat leads to excess aldosterone, other than a disorder of the adrenal glands. It could becaused by any condition that decreases blood flow to the kidneys, decreases bloodpressure, or lowers sodium levels. Secondary aldosteronism may be seen with congestive
heart failure, cirrhosis of the liver, kidney disease and toxemia of pregnancy (pre-eclampsia).
Low aldosterone (hypoaldosteronism) usually occurs as part of adrenal insufficiency. It
causes dehydration, low blood pressure, a low blood sodium level, and a high potassium
level. When infants lack an enzyme needed to make cortisol, a condition called congenital
adrenal hyperplasia, they may not be able to produce enough aldosterone.
Aldosterone and renin tests are generally ordered together to evaluate whether the adrenal
glands are producing appropriate amounts of aldosterone and to distinguish between thepotential causes of excess or deficiency. Typically they are quantitative plasma or serum
assays that are based on competitive EIA principles.

Reagents for Immunoassay Development
E30451M

MAb to Aldosterone• Cross-reactivity: Androstenedione (<0.01%),
Corticosterone (<0.01%), &Desoxycorticosterone (<0.01%)

Suitable for use in
ELISA

www.meridianlifescience.com

4Anti-Müllerian Hormone (AMH)
Anti-Müllerian Hormone (AMH) is a glycoprotein hormone structurally
related to inhibin and activin and is part of the transforming growth
factor beta superfamily. It is expressed by granulosa cells of theovary during a female’s reproductive years and plays a key role ingrowth differentiation and folliculogenesis. Specifically, AMH
expression inhibits primordial follicle recruitment and decreases
the sensitivity of follicles for the FSH-dependent selection. Besides
its functional role in the ovary, AMH serum levels also serve as abiomarker for ovarian reserve.
AMH is a dimeric glycoprotein molecule that consists of two identical subunits linked
by sulfide bridges. Each subunit contains a pro-region (pro-AMH or N-terminal) and a
C-terminal domain (also called the “mature” region) which is cleaved at monobasic sites
between the two domains. After cleavage, the pro-region (110-kDa) and C-terminal(25 kDa) homodimers remain associated in a noncovalent complex that bind to AMH
Receptor II to activate signaling.
AMH is considered an extremely sensitive marker of ovarian function and ovarian aging.
It is useful to assess conditions such as polycystic ovary syndrome and premature
ovarian failure. AMH is also a predictor for ovarian response in in vitro fertilization (IVF).
Overall, a higher level of AMH
in normal, healthy women
Anti-Müllerian Hormone (AMH)
aged 30-44 has a positive
Protein Structure
correlation with natural fertility
for spontaneous conception.
Pro-AMH
MatureDiagnostic tests that measure(N-Terminal)(C-Terminal)
AMH levels in serum or
plasma are usually quantitative
sandwich-ELISA that use
antibodies directed against
epitopes in the stable proCleavage (a.a. 451)
region and mature region.
Figure based from C. Heule, W. Salzburger, and A. Böhn,
Genetics. 196: 579–591 (2014)

AMH levels can predict Ovarian Reserve

5

Endocrine Disorders - Reagents for Assay Development

Reagents for Immunoassay Development
MAb to AMH• Capture Antibody• Recognizes the N-terminal domain of AMH• Does not cross react with human LH, FSH or
human activin A and activin B

9603

MAb to AMH• Detection Antibody• Recognizes the C-terminal domain of AMH• Does not cross react with LH, FSH or humanactivin A and activin B
AMH Recombinant• Expressed in insect cells• ≥ 90% (SDS-PAGE), 55kDa MW• Control antigen for MAb pair 9602 and 9603

PAIR

9602

9604

E01349M

PAIR

E01350M
R01713

E01347M

PAIR

E01348M
R01712

Suitable for use in
ELISA & CLIA

MAb to AMH• Capture Antibody
MAb to AMH• Detection Antibody
AMH Recombinant• Represents the full AMH sequence (MW 60 kDa)
• Expressed in E. coli
• >90% pure (SDS-PAGE)• Control antigen for MAb pair E01349M
and E01350M
MAb to AMH• Capture Antibody
MAb to AMH• Detection Antibody
AMH Recombinant• Contains a His-tag• Partial AMH sequence (MW ~32kDa)• Expressed in E. coli
• Control antigen for MAb pair E01347M
and E01348M

Suitable for use in
ELISA, CLIA & LF

www.meridianlifescience.com

6Calcitonin
Calcitonin is a polypeptide hormone that is produced by the C-cells of
the thyroid gland and it acts to reduce blood calcium and phosphatelevels, opposing the effects of Parathyroid Hormone (PTH).
Calcium is an essential structural component of the skeleton and plays a key role inmuscle contraction, blood coagulation, enzyme activity, neural excitability, secondary
messengers, hormone release, and membrane permeability. Three major hormones(PTH, vitamin D, and calcitonin) interact to maintain a constant concentration of calciumin the body.
Calcitonin is measured using quantitative
sandwich immunoassays that employ
MAbs for the recognition of intact
and mature calcitonin. High levelsof calcitonin identify patients withnodular thyroid diseases and diagnose
medullary thyroid cancers which originate
from the C-cells of the thyroid gland.
Medullary tumors are the third mostcommon of all thyroid cancers.

PAIR

Reagents for Immunoassay Development

7

E01357M

MAb to Calcitonin• Produced in Cell Culture• Capture Antibody

E01356M

MAb to Calcitonin• Produced in Cell Culture• Detection Antibody

Endocrine Disorders - Reagents for Assay Development

Suitable for use in
CLIA, ELISA & RIA

Cortisol
Cortisol is the primary glucocorticoid secreted by the adrenal glandin response to ACTH stimulation, stress, or low blood-glucose
concentration. It functions to increase blood sugar throughgluconeogenesis, to suppress the immune system, and to aid in themetabolism of fat, protein, and carbohydrates. It also decreases boneformation. It is secreted in a diurnal pattern with levels rising in theearly morning, peaking around 8 am, and flattening in the evening.
The production of too much cortisol can cause Cushing’s
syndrome which, if left untreated, can lead to serious healthproblems such as heart attack, stroke, blood clots and Type 2
diabetes. The most common cause of Cushing’s syndromeis the long-term, high-dose use of the cortisol-like
glucocorticoids which are used to treat other medical
conditions like asthma, rheumatoid arthritis, and lupus.
The second most common cause is pituitary tumors ora tumor on the adrenal gland itself.
Too little cortisol can be caused by Addison’s disease(also called primary adrenal insufficiency), a condition inwhich your adrenal glands do not function well due toautoimmune disorders, tumors, or infections liketuberculosis or HIV.
Cortisol disorders are generally diagnosed using competitive
quantitative immunoassays from urine, saliva, or blood samples.

Reagents for Immunoassay Development
E01332M

E86322M

E86322M

MAb to Cortisol• Cross-reactivity: Prednisolone (5.6%),
11-Deoxycortisol (0.9%), Corticosterone (0.6%),
11-Deoxycorticosterone (<0.1%), Progesterone(<0.1%), 17-Hydroxyprogesterone (<0.1%),
Testosterone, Estradiol & Estriol (<0.1%), Danazol(<0.01%)• Produced in Cell Culture
MAb to Cortisol• Recognizes cortisol-BSA conjugate and free cortisol• No cross-reactivity with BSA• Cross-reactivity: Corticosterone (20%)

Suitable for use inCompetitive ELISA

MAb to Cortisol• Recognizes cortisol-BSA conjugate and free cortisol• No cross-reactivity with BSA• Cross-reactivity: Corticosterone (49%)

www.meridianlifescience.com

8

Dehydroepiandrosterone (DHEA)
Dehydroepiandrosterone (DHEA) is one of the most abundantcirculating steroids and is produced in the adrenal glands, the gonads,
and the brain, where it functions as a metabolic intermediate in thebiosynthesis of the androgen and estrogen sex steroids. Its production
is controlled by ACTH and the majority of DHEA is secreted as
3-sulfoconjugate dehydroepiandrosterone sulfate (DHEAS). In thecirculation, DHEA and DHEAS are mainly bound to albumin, with asmall amount bound to sex hormone-binding globulin (SHBG).
Elevated DHEA/DHEAS levels caused by androgen-producing adrenal tumors can cause
symptoms of hyperandrogenism in women. Men are usually asymptomatic, however
peripheral conversion of androgens to estrogens can occasionally produce mild
estrogen excess. In small children, excessive DHEA/DHEAS levels can be due tocongenital adrenal hyperplasia (CAH) caused by 3 beta-hydroxysteroid dehydrogenase
deficiency, 21-hydroxylase deficiency (the most common form of CAH) or 11 betahydroxylase deficiency.
Serum DHEAS diagnostic assays are used to help evaluate adrenal gland function,
to detect adrenal tumors or cancers, and to help determine the cause of masculine
physical characteristics (virilization) in girls and women or early puberty in boys.
The test may also be used with other hormone tests to rule out certain diseases of
the testes or ovaries.

Reagents for Immunoassay Development
E01236M

9

MAb to DHEA
• Reacts with DHEA and DHEAS• Cross-reactivity: Cholesterol (<1%), Progesterone(<1%), Hydrocortisone (<1%), Estradiol (<1%)
and Vitamin D3 (<1%)• HPLC purified

Endocrine Disorders - Reagents for Assay Development

Suitable for use in
ELISA

Estradiol (E2)
Estradiol, also known as E2 or 17β-estradiol, is an estrogen steroid
hormone and the major female sex hormone. It is involved in theregulation of female reproductive cycles and it is responsible for thedevelopment of female secondary sexual characteristics.
Estradiol is predominately produced within the follicles of the ovaries and uponmenopause in women, production of estrogens by the ovaries stops and estradiol levels
decrease to very low levels. Low levels of estradiol can lead to loss of bone mass andfertility challenges in both sexes, premature menopause, depression and prematureskin aging. Abnormally high levels of estradiol can result in early puberty in both sexes,
development of breast tissue in males (gynecomastia), and may drive ovarian, breastand endometrial cancer.
Testing for Estradiol is useful to diagnose cases of infertility, abnormal menses, and tomonitor follicular development during assisted reproduction protocols. Typically, aquantitative serum test is sufficient to make a differential diagnosis and subsequent
treatment plan.

Reagents for Immunoassay Development
MAS04-267

E86022M

MAb to Estradiol• Cross-reactivity: Estriol (2.3%), Estrone (0.17%),
Testosterone (0.01%), Androstanedione (<0.005%),
Cortisone (<0.005%), Cortisol (<0.005%),
Progesterone (<0.005%), Corticosterone (<0.005%)
MAb to 17-beta-Estradiol• Reacts with 17-beta-Estradiol-BSA conjugate andfree Estradiol• No cross-reactivity with BSA

Suitable for use inCompetitive ELISA

www.meridianlifescience.com

10Estriol (E3)
Estriol (E3) is one of three major estrogens, the others being estradiol(E2) and estrone. Its levels are only detectable during pregnancy
where it is synthesized in very high quantities by the placenta (levels
increase 100-fold during pregnancy). Its role is to ensure a quiescentuterus during prelabor and as such it can be used as a marker of fetal
health and well-being.
During pregnancy, 90 to 95% of estriol in the maternal circulation is conjugated in theform of estriol glucuronide and estriol sulfate, and levels of unconjugated estriol areslightly less than those of unconjugated estradiol and similar to those of unconjugatedestrone. If levels of unconjugated estriol (free estriol) are abnormally low in a pregnant
woman, this may indicate chromosomal or a congenital anomaly like Down syndrome or
Edward’s syndrome. Estriol is included as part of the triple test and quadruple test forantenatal screening for fetal anomalies. However, because many pathological conditions
in a pregnant woman can cause deviations in estriol levels, screening tests are often
seen as less definitive of fetal-placental health than a nonstress test. Conditions which
can create false positives and false negatives in estriol testing for fetal distress include
pre-eclampsia, anemia, and impaired kidney function.
Estriol tests are usually a solid-phase competitive immunoassay that quantitatively
measures unconjugated estriol in serum.

Reagents for Immunoassay Development

11

E01360M

MAb to Estriol (E3)
• Produced in Cell Culture

Suitable for use inCompetitive ELISA,
CLIA & RIA

A82605P

Estriol (E3) Antigen, HRP conjugated• Estriol linked at the 6 position• Buffer: Tris containing protein stabilizers

Suitable for use in
ELISA

Endocrine Disorders - Reagents for Assay Development

Follicle-stimulating hormone (FSH)
Follicle-stimulating hormone (FSH) is a gonadotropin secreted by
the anterior pituitary glands and regulates the activity of the gonads(e.g. ovaries and testes). Specifically, it operates in conjunction withluteinizing hormone (LH) to stimulate the development of graafianfollicle in females, and promote the development of the tubules inthe testes and the differentiation of sperm.
The production and secretion of FSH and LH are regulated by a balance of positive andnegative feedback mechanisms involving the hypothalamic-pituitary axis, the reproductive
organs, and the pituitary and sex steroid hormones.
In women, follicle stimulating hormone levels start to rise naturally around the menopausal
period, reflecting a reduction in function of the ovaries and a decline of oestrogen andprogesterone production. However, at any other time, an increase in FSH levels are a sign ofmalfunction in the ovary or testis. A wide variety of disorders are associated with high FSH
including premature ovarian failure, gonadal dysgenesis, systemic lupus erythematosus,
testicular failure and klinefelter syndrome. Low levels of FSH are also problematic and canlead to incomplete development at puberty for both men and women.
FSH is typically measured through a quantitative serum test. Only third generation FSH
testing is sensitive enough (0.03 mIU/mL) to assess gonadal dysfunction in children
18 years and under.

PAIR

Reagents for Immunoassay Development
MAF02-264 MAb to FSH• Reacts with intact FSH molecule• Does not cross-react with other alpha hormones• Capture Antibody
MAF02-243 MAb to FSH• > 90% pure (SDS-PAGE)• Detection Antibody

Suitable for use in
ELISA

H6F02-323 FSH > 98%• Activity: 6,217 IU/mg• Contaminants: hTSH (0.13%), hLH (<1%), hGH(0.0001%), PRL (0.0062%)• Lyophilized
E87701M MAb to Human FSH (Intact)• Recognizes the c1 epitope of intact FSH• Not cross-reactive with FSH Beta
• Capture or Detection Antibody

Suitable for use in
ELISA & RIA

www.meridianlifescience.com

12Glucagon
Glucagon is a peptide hormone produced by the alpha cells of
the pancreas. Its primary function is to elevate the concentrationof glucose in the blood by promoting gluconeogenesis andglycogenolysis. It also decreases fatty acid synthesis in adipose
tissue and the liver, as well as promotes lipolysis in these tissues,causing them to release fatty acids into circulation where they canbe catabolized to generate energy. Its effect is opposite to thatof insulin, which lowers the extracellular glucose.
Glucagon belongs to the secretin family of hormones and together with insulin, it forms
part of the feedback system that keeps blood glucose levels stable. Glucagon increases
energy expenditure and is elevated under conditions of stress.
A glucagon diagnostic assay is primarily useful for detecting a glucagon-secreting tumorof the pancreas (glucagonomas). Glucagonoma tumor cells produce large amounts ofglucagon, and these high levels create severe, painful, and life-threatening symptoms.
About 5-10% of neuroendocrine tumors that develop in the pancreas are glucagonomas
and 75% of the time these glucagonomas are malignant.
Glucagon diagnostic tests are typically quantitative two-site sandwich immunoassays
and the performance between manufacturers can vary significantly due to antibody
cross reactivity. It is important to select highly specific antibodies to glucagon in order
reduce the potential for cross-reactive binding to other circulating pro-glucagon derived
peptides, such as glicentin. In addition, cross-reactivity can occur with different isoformsof glucagon, not all of which are biologically active. Some diagnostic assays remove thebiologically inactive isoforms before measurement, while others do not.

Reagents for Immunoassay Development
D24460R

D01234G

13

MAb to Glucagon-Like Peptide 1• Specific for the C-terminal of GLP-1• Does not cross-react against GLP2, GRPP, PYY,
VIP, PHI, GIP, Glucagon or Oxyntomodulin• Produced in Cell Culture and Lyophilized

Suitable for use
in Dot Blot & IHC

PAb to Human Glucagon• Specific for the N-terminal of human Glucagon• Produced in Goat
• >95% pure (Protein G purified)

Suitable for use in
ELISA

Endocrine Disorders - Reagents for Assay Development

Human chorionic gonadotropin (hCG)
Human chorionic gonadotropin (hCG) is a heterodimeric glycoproteinwith an alpha subunit identical to that of LH, FSH and TSH, and aunique beta-subunit. It is a hormone produced by cells of the fetal
placenta and it maintains the function of the corpus luteum during
the first 3 to 4 months of pregnancy.
hCG is secreted as soon as the trophoblast implants in the uterine wall and it is at its
highest concentration between the 7th and 10th weeks of pregnancy. The urine ofwomen in the first month of pregnancy contains enough hCG to be detected by ELISA.
Total hCG is commonly used to confirm and monitor pregnancy, but it also has clinicalutility in detecting miscarriages, preeclampsia, neoplasms, trophoblastic diseases, andrisk calculations for Trisomy-21 and Trisomy-18.
Some cancerous tumors also produce beta-hCG and accordingly, free beta-hCG has
some use as a tumor marker in gestational trophoblastic disease, certain testiculartumors, where the ratios of the free β subunit to intact hCG can be quite high. Free
β-hCG may also have clinical utility in first-and second-trimester prenatal screening for
Down syndrome and other chromosomal anomalies.
Qualitative blood tests based on lateral flow technology can detect hCG levels as low
as 10 mIU/mL-100ml U/mL, depending on the brand. Each of these assays are basedon the two-site sandwich enzyme immunoassay principles and are prone to possible
false-positive results from heterophilic antibodies.

PAIR

PAIR

Reagents for Immunoassay Development
MAF05-043 MAb to hCG beta
• Recognizes the beta subunit of intact hCG• Does not cross-react with hCG alpha subunit
MAF05-627 MAb to hCG beta
• Cross-reactivity: LH (0.5%)• Detection Antibody
E86820M MAb to hCG beta
• Specifically recognizes the beta subunit of hCG• Does not cross-react with hLH, hTSH or hFSH
• Capture Antibody
E86308M MAb to hCG beta
• Specifically recognizes the beta subunit of hCG• Does not cross-react with hLH, hTSH and hFSH
• Detection Antibody

Suitable for use in
ELISA

Suitable for use in
ELISA & LF

www.meridianlifescience.com

14

Human chorionic gonadotropin (hCG) continued

PAIR

Reagents for Immunoassay Development continued
D01241G PAb to Intact hCG• Reacts with alpha subunit and beta subunit of thehCG molecule• Produced in Goat
• >98% pure (SDS-PAGE)• Pairs with E01323M in a free beta-hCG sandwich
immunoassay
E01323M MAb to hCG beta
• Recognizes the free beta hCG subunit• Does not cross-react with the alpha subunit• Cross-reactivity: intact hCG (0.5%)• Pairs with Catalog #D01241G in a free beta-hCG
sandwich immunoassay
MAF05-023 MAb to hCG beta
• Recognizes the beta subunit of intact hCG• Cross-reactivity: Free beta subunit (49.0%),
Free alpha subunit (0%), LH (38.4%),
FSH (3.7%), TSH (3.7%)
MKF05-148 MAb to hCG beta
• Not cross-reactive with LH, FSH or TSH
A01376H hCG Native Antigen• Sourced from human urine• > 98% pure (SDS-PAGE)• Lyophilized from 50 mM Ammonium Bicarbonate
A01322H hCG Native Antigen• USP grade, Activity: 14,800 IU/mg (Bio-assay)• Buffer: Sodium Chloride

15

Endocrine Disorders - Reagents for Assay Development

Suitable for use in
ELISA

Human growth hormone (hGH)
Human growth hormone (hGH), also known as somatotropin, is a
peptide hormone that stimulates growth, cell reproduction, and cellregeneration and is essential for normal growth and development inchildren. In adults, growth hormone plays a role in regulating bonedensity, muscle mass, and glucose and lipid metabolism. It can also
affect heart and kidney function.
Growth hormone is produced by the pituitary gland and is normally released into thebloodstream in pulses throughout the day and night. As a result, obtaining a single
measurement of GH in blood is difficult to interpret and not clinically useful. GH
stimulation and suppression tests are therefore often used to diagnose GH abnormalities.
Growth hormone deficiency (GHD) is a rare disorder characterized by the inadequate
secretion of GH and it can result from congenital abnormalities or from damage to thepituitary gland caused by a head injury, brain tumor, or surgery or radiation treatment.
Childhood-onset GHD results in growth retardation, short stature, and maturation
delays. Adult-onset GHD is characterized by a number of variable symptoms including
reduced energy levels, altered body composition, osteoporosis (reduced bone mineraldensity), reduced muscle strength, lipid abnormalities such as increased LDLcholesterol, insulin resistance, and impaired cardiac function. Excess GH is most often
due to a GH-secreting pituitary tumor (usually benign) and can result in acromegaly(gigantism) in children. Among the most serious symptoms of acromegaly are type 2
diabetes, high blood pressure, increased risk of cardiovascular disease, and arthritis.
To determine hGH levels, dynamic tests are required for proper diagnosis. These tests aremeant to stimulate the pituitary (via insulin, arginine, clonidine and l-dopa) to secrete GH
allowing for the testing of blood samples at timed intervals. hGH assays are based on the
two-site sandwich enzyme immunoassay principles using monoclonal antibodies or a
combination of monoclonal and polyclonal antibodies. One challenge faced with hGH
assays is that the normal composition of hGH in blood is actually mixture of different
isoforms, present at constant relative proportions. The primary isoform is a 22 kD moleculeand a 20 kD molecule, as well as hetero- and homodimers and multimers. Assay results
can vary considerably depending on reactivity with various isoforms.

PAIR

Reagents for Immunoassay Development
MAF06-154 MAb to hGH• Cross-reactive with Human Placental Lactogen(<0.02%) and Human Prolactin (<0.02%)• Capture Antibody
MAF06-210 MAb to hGH• Cross-reactive with Human Placental Lactogen(<0.02%) and Human Prolactin (<0.02%)• Detection Antibody

Suitable for use in
ELISA

A75714H hGH Native Antigen• Cross-reactivity: FSH (<0.01%), LH (<0.01%), TSH(<0.01%) and Prolactin (<0.01%)• >80% pure (SDS-PAGE)• Lyophilized from 50 mM Ammonium Bicarbonate

www.meridianlifescience.com

16

Insulin (C-peptide)
C-peptide and the hormone insulin are created from a larger
molecule called proinsulin and stored in the beta cells of thepancreas. Intact proinsulin undergoes enzymatic cleavage to
become des-31,32-proinsulin and des-64,65-proinsulin andeventually, insulin and C-peptide (an inactive peptide chain).
Although insulin and C-peptide are secreted in equimolar amounts from beta-cells,
C-peptide has a longer half-life and is present in peripheral blood in higher molar
concentrations than insulin, making it less prone to marked fluctuations. The
measurement of plasma insulin, C-peptide and proinsulin concentrations has been
identified as the most useful test in identifying the cause of hypoglycaemia.
Type 1 and type 2 are the two most common types of diabetes and although bothof the types are characterized by high blood glucose, the pathogenesis between thetwo differ. The insulin/C-peptide test is useful in order to differentiate insulin-dependentpatients from non-insulin-dependent patients. Overall raised plasma insulin, C-peptide,and proinsulin concentrations in the presence of hypoglycemia can indicate
endogenous hyperinsulinaemia which is often seen in people with early stage type 2
diabetes mellitus. In general, c-peptide is considered to be a reliable marker of residual
beta-cell function and serum or urine C-peptide determinations, in conjunction withblood glucose and insulin levels, aid in the differential diagnosis of hypoglycemia.
Early assays for insulin, proinsulin, and C-peptide were competitive RIAs, however mostcommercial assays are now competitive (Insulin and C-peptide) or two-site enzyme
immunoassays (total of intact proinsulin).

Reagents for Immunoassay Development
MHP18-601

17

Mab to C-peptide• Reacts with C-peptide of human proinsulin• > 98% pure (sequential precipitation using
caprylic acid and ammonium sulphate)

Endocrine Disorders - Reagents for Assay Development

Suitable for use inCompetitive ELISA

Luteinizing hormone (LH)
Luteinizing hormone (LH) is a gonadotropic hormone that is
produced by the pituitary gland and operates in conjunction with FSH
to drive puberty, menstruation and fertility. A surge in LH levels triggers
ovulation and the development of the corpus lutem in females, and inmales it causes the Leydig cells to release testosterone.
LH is composed of two noncovalently associated dissimilar amino acid chains, alpha andbeta. The alpha chain is similar to that found in human thyroid-stimulating hormone (TSH),
follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG).
LH diagnostic assays are useful to evaluate fertility issues, the function of reproductive
organs (ovaries or testicles), or to detect ovulation. In children it can also be useful toevaluate early or delayed sexual maturation. The most sensitive LH assays on the market
are monoclonal antibody based competitive-ELISAs. However, lateral flow devices using
antibody sandwich-based formats are widely used for at-home ovulation tracking.

PAIR

PAIR

Reagents for Immunoassay Development
MAF07-226 MAb to LH• Cross-reactivity: hCG (<0.01%), FSH (>0.1%)• Capture Antibody
MAF07-036 MAb to LH• > 90% pure (SDS-PAGE)• Detection Antibody
MAF07-412 MAb to LH• Cross-reactivity: hCG (<0.01%), FSH (>0.1%)• Capture Antibody

Suitable for use in
ELISA

MAF07-037 MAb to LH• > 90% pure (SDS-PAGE)• Detection Antibody
A01687H LH > 98% pure
• Sourced from human pituitary glands• Activity: 15,600 IU/mg• Contaminants (<0.5% w/w): FSH (1 IU/mg),
hGH (<0.001 mg/mg), PRL (<0.001 mg/mg),
TSH (<0.01 IU/mg)• Lyophilized from 50 mM Ammonium Bicarbonate

Suitable as a Control
or Calibrator for EIA
Assayswww.meridianlifescience.com

18

Parathyroid Hormone (PTH)
Parathyroid Hormone (PTH) is secreted by the parathyroid gland andplays an important role in bone remodeling. It also works together
with Vitamin D to maintain healthy bones. PTH is secreted in response
to low calcium levels as a 118 amino acid polypeptide that undergoes
two successive cleavages to yield an 84 amino acid biologically active
hormone. It has a very short half-life of less than five minutes andbreaks down into various fragments of which the biologicalsignificance remains to be defined.
A PTH blood test is useful in the differential diagnosis of overactive parathyroid glands(hyperparathyroidism). Primary hyperparathyroidism is most often caused by a benign tumor
in one or more of the parathyroid glands and patients with this condition have high PTH andcalcium levels. Secondary hyperparathyroidism is often seen in patients with chronic renal
failure (CRF). The kidneys fail to excrete sufficient phosphate, and the parathyroid glandsecretes PTH in an effort to lower calcium levels to balance the calcium-phosphate ratio.
Tertiary hyperparathyroidism occurs when CRF causes a severe imbalance in the calciumphosphate ratio, leading to very high PTH production that results in hypercalcemia.
Current blood tests generally measure intact PTH by ELISA. Most assays use two
antibodies in sequence, the first recognizing the N-terminal and the second the C- terminal.

Reagents for Immunoassay Development
E01306M

MAb to PTH (a.a. 1-34)• Reacts with a.a. 1-34 of human PTH

Suitable for use
in ELISA & RIA

E24101M

MAb to PTH (a.a. 53-68)• Specific for human PTH peptide a.a. 53-68• Does not cross-react with Synthetic human PTH
peptide (a.a. 1-10, a.a. 1-34 and a.a. 1-38)• Lyophilized

Suitable for use in
ELISA, IHC &Immunoluminetric

MAb to PTH (a.a. 53-84)• Does not cross react with synthetic human PTH
peptide (a.a. 1-10, a.a. 1-34 and a.a. 1-38)• Produced in Cell Culture• Lyophilized from 0.1 M Phosphate Buffered Saline,
pH 7.4

Suitable for use in
ELISA & IHC

E24150M

19

Endocrine Disorders - Reagents for Assay Development

Pregnancy Associated Plasma Protein-A (PAPP-A)
Pregnancy associated plasma protein-A (PAPP-A) is as a glycoprotein
found in the serum of pregnant women. It is produced by the placenta
and circulates in the form of a heterotetramer complexed witheosinophil major basic protein (proMBP). Its main role in pregnancy
is to prevent the recognition of the fetus by the maternal immune
system. In non-pregnant individuals, PAPP-A can also be produced byseveral cell types and detected at much lower levels (and in the formof a homodimer). Studies have shown that it can also be used as apotential biomarker for plaque instability in cardiac disease.
In the first trimester of pregnancy, PAPP-A assays are used in conjunction with hCG
or free beta hCG to screen for Down syndrome (Trisomy 21) and Edward’s syndrome(Trisomy 18). A low PAPP-A level can also be associated with pregnancy complications
such as fetal growth restriction, fetal demise, preterm birth, and pre-eclampsia in thethird trimester. As a cardiac marker, high PAPP-A serum levels indicate a risk for acutecoronary syndromes (unstable angina and acute myocardial infarction), as PAPP-A isexpressed by unstable and ruptured coronary artery plaques, but not stable plaques.
Serum PAPP-A assays are based the two site sandwich immunoassay principles. For
developing PAPP-A pregnancy diagnostics, antibodies that detect the heterotrimeric
form (htPAPP-A/proMBP complex) must be used whereas antibodies that detect thehomodimeric subunit dPAPP-A should be used for cardiac assays.

Reagents for Immunoassay Development
MAb to PAPP-A (proMBP)• Detection Antibody

E86141M

MAb to PAPP-A• Recognizes human PAPP-A subunit of htPAPP-A• Can detect htPAPP-A and dPAPP-a in sandwich
immunoassay when paired with Catalog #E86901M• Capture antibody

E86910M

MAb to PAPP-A• Recognizes human PAPP-A subunit of htPAPP-A• Detection antibody

A86864H

PAPP-A Native Antigen• Represents a heterotetrameric complex consistingof PAPP-A and pro-MBP subunits• Sourced from pooled human retroplacental blood• Lyophilized from 10 mM Tris-HCl, 0.15 M Sodium
Chloride, pH 7.5

PAIR

E86114M

Suitable for use in
ELISA

www.meridianlifescience.com

20

Progesterone (P4)
Progesterone (P4) belongs to a group of steroid hormones called theprogestogens and it is the major progestogen in the body. Its main role
is to maintain pregnancy and to regulate the female’s menstrual cycle.
Its levels increases sharply during the luteal phase of the menstrual
cycle as it works to thicken the lining of the uterus to prepare it for a
fertilized egg each month.
In the event that an egg does implant, progesterone’s function is to maintain the uterine lining
throughout the pregnancy and the levels increase from 9 to 32 weeks. If no fertilized egg implants,
progesterone levels drop and menstruation begins. Progesterone is also necessary for breast
development and breastfeeding and complements some effects of estrogen. In men it works withtestosterone to help in sperm development.
Progesterone diagnostic assays are useful to help determine the cause of infertility, track ovulation,
help diagnose an ectopic or failing pregnancy, monitor the health of a pregnancy, monitor
progesterone replacement therapy, and help diagnose the cause of abnormal uterine bleeding.
A related progestogen steroid hormone is 17-Hydroxyprogesterone (17-OH P) which is synthesizedfrom progesterone and 17-Hydroxypregnenolone. It functions as a precursor of cortisol in theadrenal glands or can be converted into androgenic and estrogenic hormones. Measurement of
17-OH P is used as an aid in the diagnosis and treatment of various disorders of the adrenal glands
or the ovaries. It is also part of newborn screening in many countries used to detect congenital
adrenal hyperplasia (CAH). Progesterone assays are usually quantitative and based on thecompetitive immunoassay format.

Reagents for Immunoassay Development
E82321M

E86222M

E86702M

A82606P

21

MAb to Progesterone
Cross-reactivity:
1 μg cortisol= 5 ng
10 ng estradiol < 5 ng

500 ng estriol< 5 ng20 ng testosterone < 5 ng

MAb to Progesterone• Cross-reactive with 17-Hydroxyprogesterone (10%)
and Deoxycorticosterone (1%)
MAb to Progesterone• Cross-reactive with 17-Hydroxyprogesterone (1%) andDeoxycorticosterone (1%), 11-Hydroxyprogesterone(25%), Deoxycortisol (0.03%), 5-alpha-pregnane-3,20dionene (10.5%), Cortisol (0.002%), Corticosterone(0.01%)
Progesterone 3-HRP Conjugate• Progesterone antigen linked at the 3 position with HRP• MW ~ 45 kDa• Buffer: Tris, pH 7.6 containing protein stabilizers and0.005% Thimerosal

Endocrine Disorders - Reagents for Assay Development

Suitable for use incompetitive ELISA

Prolactin (PRL)
Prolactin (PRL) is a protein that is best known for its role in initiating
and maintaining lactation in mammals. It is secreted by the anterior
pituitary gland and controlled by the hypothalamus.
High levels of prolactin are normal during pregnancy and postpartum during breastfeeding,however consistently elevated levels (>30 ng/mL) in the absence of a pregnancy are
indicative of hyperprolactinemia, the most common hypothalamic-pituitary disorderencountered in clinical endocrinology. Hyperprolactinemia caused by prolactin-secreting
pituitary adenoma, primary hypothyroidism, renal insufficiency, polycystic ovarian disease,and ectopic tumors. It often results in galactorrhea (unexplained flow of breast milk),
amenorrhea, and infertility in females, and in impotence and hypogonadism in males.
Certain medications and some common stressors can also raise prolactin levels.
The biologically active form of prolactin is the 23-kDa monomeric polypeptide secretedby the pituitary gland; however, circulating prolactin exists in a number of additional
forms. Big prolactin (60 kDa) and macroprolactin (150 kDa), which are present in serumin varying quantities, can cause apparent hyperprolactinemia, but they have no clinicalimportance because they exhibit little biological activity. Several studies have shownthat prolactin two-site sandwich immunoassays are susceptible to interference frommacroprolactin. Current best practice recommends that all sera with increased total
prolactin concentrations be subfractionated by PEG precipitation to measure thebioactive monomeric prolactin concentration, a more clinically meaningful variable.

PAIR

PAIR

Reagents for Immunoassay Development
MAF10-111 MAb to Prolactin• Cross-reactivity: hGH (<0.1%)• Capture Antibody
MAF10-145 MAb to Prolactin• Detection Antibody
E20122M MAb to Human Prolactin• Cross-reactivity: hCG-b, hCG, hLH,
hFSH is (<1%) & Prolactin (100%)• Capture or Detection Antibody

Suitable for use in
ELISA

E20630M MAb to Human Prolactin• MW 23 kD• Capture or Detection Antibody
A01274H Human Prolactin, Recombinant• Lyophilized

www.meridianlifescience.com

22Testosterone
Testosterone is the primary male sex hormone and an anabolic
steroid. In male humans, it is secreted by the testes and it plays a
key role in the development of male reproductive tissues, as wellas promoting secondary sexual characteristics such as increased
muscle and bone mass, and the growth of body hair.
In addition, testosterone is involved in health and well-being, and the prevention of
osteoporosis. Testosterone in also important in women and it is produced by the ovaries
and the adrenal glands. It is best known for its role in a promoting a woman’s sex drive
or libido however, it is also important for bone strength and the development of leanmuscle mass.
In males, a testosterone assay is used to detect hypogonadonism, testicular failure,
infertility, hypopituitarism and hyperprolactinemia. In females, it can be used to diagnose
polycystic ovary syndrome, adrenal hyperplasia, infertility, hirsutism, amenorrhea, obesityand virilization. Typically, a test for total testosterone is used – this test measures
testosterone that is bound to proteins in the blood (e.g., albumin and sex-hormone binding
globulin [SHBG]) as well as testosterone that is not bound (free testosterone). About
two-thirds of testosterone circulates in the blood bound to SHBG and slightly less than
one-third bound to albumin. A small percent (less than 4%) circulates as free testosterone.
Free testosterone plus the testosterone bound to albumin is the bioavailable testosterone,
which can act on target tissues.
In many cases, the total testosterone test provides adequate information. However,in certain cases, for example when the level of SHBG is abnormal, a test for free orbioavailable testosterone may be performed as it may more accurately reflect thepresence of a medical condition. Testosterone immunoassays are usually MAb-based
competitive ELISAs.

23

Endocrine Disorders - Reagents for Assay Development

Reagents for Immunoassay Development
E30412M

MAb to Testosterone
Cross-reactivity: Testosterone (100%), 11-betaHydroxy testosterone (3.3%), 17-alpha-Methyl
Testosterone (<0.1%), 5-alpha DHT (0.8%),
Estradiol (<0.1%), Progesterone (<0.1%)

E82721M

MAb to Testosterone• Cross-reactive at D50 with 5-Androsten-3betaol-17-one (0.6%), 5-alpha-Androst-1-ene-3,
17-dione (0.5%), Androsterone (3.6%), 5-alphaDihydroxytestosterone (10%), Corticosterone(<0.7%), DHEA (1.2%)• Less than 0.1% cross-reactivity to 4-cholesten3-one, 5-cholesten-3-beta-ol., Cholesterol,
Cortisone, Deoxycorticosterone, Dexamethasone,
16- epiestriol, 17-alpha estradiol, 17-beta-estradiol,
Estrone, Isoandrosterone, 5-alpha-pregnandione,
Prednisolone, Prednisone, 17-alpha-OHprogesterone, 20-alpha-OH progesterone,
21-OH-progesterone, Progesterone,
Reichestein’s substance

E86902M

MAb to Testosterone• Cross-reactivity with 11-beta-Hydroxy testosterone(3.3%), 17-alpha-Methyl Testosterone (<0.1%),
5-alpha DHT (0.8%), Estradiol (<0.1%),
Progesterone (<0.1%)

E45703M

MAb to Testosterone• Cross-reactive with 5-alpha-Dihydrotestosterone(6.6%)

A01312B

Testosterone Synthetic Antigen, BSA Conjugated• Testosterone-3-BSA Conjugate• Labeling Ratio: 15:1
• Concentration 10.0 mg/mL• Buffer: 10 mM Phosphate, pH 7.0 with 0.05%
Sodium Azide

Y82230P

Testosterone Synthetic Antigen, HRP Conjugated• Testosterone-3-HRP Conjugate• Buffer: 0.05 M Tris, pH 7.0 containing protein
stabilizers (0.005% Thimerosal)

Suitable for use inCompetitive ELISA

www.meridianlifescience.com

24Thyroglobulin (Tg)Thyroglobulin (Tg) is a dimeric protein produced by the follicular
cells of the thyroid and accounts for approximately half of theprotein content of the thyroid gland. It is the precursor for the
thyroid hormones T3 and T4 and each Tg molecule forms
approximately 10 thyroid hormone molecules.
The major clinical use of a Tg diagnostic test is to monitor patients with welldifferentiated thyroid cancers (particularly papillary or follicular thyroid cancer). The
measurement of thyroglobulin, after thyroidectomy and ablation of the thyroid gland,
is useful to determine metastasis. Tg assays can also be used to detect deficient Tg
synthesis in infants with goitrous hypothyroidism, which is a preventable cause ofmental retardation.
Tg serum levels are measured by two-site sandwich immunoassays. However,quantitative Tg serum assays are prone to interference from endogenous circulating
autoantibodies (anti-Tg) and can cause inappropriately low Tg results in about 20% to
30% of patients. In suspected cases of interference, a confirmatory test detecting thepresence of anti-Tg should be carried out.

PAIR

Reagents for Immunoassay Development
E01326M

MAb to Thyroglobulin• Specifically recognizes human thyroglobulin• Cross-reacts with bovine thyroglobulin.
• Does not interfere with anti-thyroglobulin
autoantibodies• Capture Antibody

E01325M

MAb to Thyroglobulin• Specifically recognizes human thyroglobulin.
• Does not interfere with anti-thyroglobulin
autoantibodies• Detection Antibody

A86852H

25

Thyroglobulin Native Antigen• Purified from human thyroid extract• >90% pure (SDS-PAGE)• Lyophilized from 25 mM Tris-HCl, pH 8.0

Endocrine Disorders - Reagents for Assay Development

Suitable for use in
ELISAThyroid Peroxidase (TPO)
Thyroid Peroxidase, also known as Thyroperoxidase (TPO), is an
enzyme involved in thyroid hormone synthesis, catalyzing theoxidation of iodide on tyrosine residues in thyroglobulin for thesynthesis of triiodothyronine (T3) and thyroxine (T4). It is oneof the most important thyroid gland antigens and along withthyroglobulin (Tg), it is targeted by the immune system in several
autoimmune thyroid diseases.
Anti-TPO assays are useful in differentiating thyroid autoimmune disorders from nonautoimmune goiter or hypothyroidism. Anti-TPO antibodies are detectable in mostautoimmune disorders including Hashimoto thyroiditis and Grave’s disease and may
also be found in patients with non-thyroid autoimmune diseases such as pernicious
anemia, type I diabetes, or other disorders that activate the immune system. Many
clinical endocrinologists use the TPO antibody test as a diagnostic tool in deciding
whether to treat a patient with subclinical hypothyroidism, as the presence of TPO
antibodies is associated with an increased risk of developing overt hypothyroidism.

PAIR

Reagents for Immunoassay Development
E01310M

MAb to TPO• Recognizes native and recombinant TPO• Also suitable for WB• Capture Antibody

E01311M

MAb to TPO• Recognizes native and recombinant TPO• Also suitable for WB• Alternative Capture Antibody

E01309M

MAb to TPO• Recognizes native and recombinant TPO• Detection Antibody

H6T09-212

TPO Recombinant Antigen• Produced in Sf9 cells• >96% pure (SDS-PAGE)• Buffer: 40 mM Tris-HCl, pH 7.6 containing 144
mM Sodium Chloride, 0.08 mM Potassium Iodide
and 20%Glycerol

A01309H

TPO Recombinant Antigen• Represents the soluble extracellular domain ofhuman TPO (a.a. 19-846)• Produced in insect cells• >95% pure (SDS-PAGE)• Lyophilized (from 20 mM Tris-HCl, pH 8.0
containing 250 mM Sodium Chloride, 0.1 mM
Potassium Iodide and 5% D-Mannitol)

Suitable for use in
ELISA & WB

www.meridianlifescience.com

26

Thyroid-stimulating hormone (TSH)
Thyroid-stimulating hormone (TSH) is a pituitary hormone that regulates
thyroid function in conjunction with thyrotropin releasing hormone (TRH).
Specifically, TSH induces production of Thyroxine (T4) which is converted
triiodothyronine (T3) and is responsible for stimulating the metabolism.
TSH is secreted throughout life but reaches high levels during the periodsof rapid growth and development, as well as in response to stress.
The release of TSH from the pituitary is regulated by the concentration of T3 and T4 in the blood.
When T3 and T4 concentrations are low, the production of TSH is increased, and, conversely,
when T3 and T4 concentrations are high, TSH production is decreased. Any imbalance in thisnegative feedback loop can be the indication of a thyroid disease, such as hyperthyroidism or
hypothyroidism. The most common cause of hypo- and hyperthyroidism is autoimmune disease.
In Hashimoto’s disease, the immune system attacks the thyroid and causes hypothyroidism.
Conversely, Grave’s disease causes an overactive thyroid leading to hyperthyroidism.
TSH serum measurements are used as a first line screening assay for hypo- and hyperthyroidism.
The capability of a TSH assay to distinguish between normal and subnormal concentrations is
critical for this thyroid testing strategy. Quantification of TSH at a lower value of 0.01 mlU/L yields
information that is useful to clinicians referring patients with subnormal TSH concentrations.

Reagents for Immunoassay Development

27

MAT04-003

MAb to TSH• Specific to intact TSH. Reacts with TSH throughthe alpha subunit/hinge region• Does not react with free beta subunit• Has limited reactivity to free alpha subunit

MAT04-410

MAb to TSH• Cross-reactivity: FSH (0.7%), LH (0.5%),
TSH alpha (1.5%)

D92409G

PAb to Human TSH beta
• <0.1% cross-reactivity with alpha hCG, LH, FSH,
growth hormone, and prolactin• Produced in Goat

A75510H

TSH Native Antigen• Produced from human pituitary glands• >95% pure (SDS-PAGE)• Contaminants: FSH (< 0.02%), LH (<0.05%), hGH(<0.05%), Prolactin (<0.05%)• Lyophilized from 50 mM Ammonium Bicarbonate

Endocrine Disorders - Reagents for Assay Development

Suitable for use
in ELISAThyroxine-binding globulin (TBG)
Thyroxine-binding globulin (TBG) is a high-affinity serum binding
protein for the thyroid hormones thyroxine (T4) and triiodothyronine(T3) and is one of three transport proteins (along with transthyretin
and serum albumin) responsible for circulating these proteins in the
bloodstream. Because TBG accounts for 76% of plasma protein
thyroxine-binding activity, an increase or decrease in its circulatinglevel alters the total concentrations of T4 and T3 in the blood,leading to potential confusion with true thyroid gland dysfunction.
TBG deficiency is a nonharmful condition that can either be acquired or genetically inherited.
The only known complications associated with TBG are those that could result fromerroneously administered treatment if TBG deficiency is misdiagnosed as another disorder.
Elevated TBG levels are associated with influences such as pregnancy, genetic
predisposition, oral contraceptives, and estrogen therapy. TBG levels can decrease withandrogenic or anabolic steroids, large doses of glucocorticoids, hypoproteinemic states,
liver disease, nephrotic syndrome, and congenital TBG variants.
Measurement of TBG is useful in distinguishing quantitative TBG derangements fromthyroid dysfunction. TBG assays are typically quantitative sandwich ELISAs.

Reagents for Immunoassay Development
MAT03-439

MAb to TBG• Affinity Constant: 1 x 10

MAT03-612

MAb to TBG• >90% pure (SDS-PAGE)

Suitable for use in
ELISA

www.meridianlifescience.com

28

Triiodothyronine (T3) And Thyroxine (T4)
Triiodothyronine (T3) and Thyroxine (T4) are hormones producedby the thyroid and are primarily responsible for regulating
metabolism. They predominately circulate bound to a carrier
protein (e.g. thyroid-binding globulin, prealbumin, and albumin),rendering them biologically inactive.
T4 is the major form of the hormone and is converted into active T3 by deiodinases(5’-deiodinase). Although circulating levels of T4 are much greater than T3 levels, 99%
of T4 remains bound and inactive. Overall, T3 possess more biological activity than T4(3-4 times more) and its effect are briefer due to its shorter half-life compared to T4.
T3 and T4 diagnostic assays complement the TSH assay, and are used to confirm a thyroid
disorder or to monitor the effectiveness of treatment. Blood tests can measure total
T4, free T4, total T3, or free T3. The total hormone concentration is dependent on theconcentration of thyroid transport proteins, therefore any conditions that affect levels of
thyroid binding proteins will affect the total (but not the free) T4 hormone levels. For
example, estrogens and acute liver disease will increase thyroid binding, while
androgens, steroids, chronic liver disease and severe illness can decrease it.
Overall, T3 levels are thought to be a more sensitive indicator of hyperthyroidism than
total T4 and it is useful in monitoring both patients under treatment for hyperthyroidism,
and in patients who have discontinued anti-thyroid drug therapy. It is especially valuable indistinguishing between euthyroid and hyperthyroid subjects. T3 levels are elevated inwomen who are pregnant, and in women receiving oral contraceptives or estrogen
treatment. Total and free T3 and T4 serum levels are measured using competitive
quantitative immunoassays.

Reagents for Immunoassay Development
E86207M

MAb to Triiodothyronine (T3)
• Reacts with both conjugate T3-BSA and free T3• Cross-reactivity with Thyroxine is (0.1%)

MAT01-121

MAb to Triiodothyronine (T3)
• Cross-reactivity: T4 (<0.28%)
MAb to Triiodothyronine (T3)
• Cross-reactivity: Triiodothyroacetic Acid(<11%) Tetraidodothyroacetic Acid (<0.30%)
MAb to Thyroxine (T4)
• Cross-reactivity: T3 (<1%), T2 (<1%), rT3 (<1%)

MAT01-237

MKT02-007
MAT02-525

29

MAb to Thyroxine (T4)
• Cross-reactivity: T3 (1.0%), rT3 (1.4%)

Endocrine Disorders - Reagents for Assay Development

Suitable for use inCompetitive ELISA

Full product list
Specificity

Catalog #

Type

Host

FormatIsotype

Adrenocorticotropic hormone (ACTH)
ACTH N-Terminal
ACTH N-Terminal
ACTH N-Terminal

E01372M
E01373M
E54057M

MAb
MAb
MAb

Mouse
Mouse
Mouse

Purified
Purified
Purified

IgG1
IgG1
IgG1

Aldosterone
Aldosterone

E30451M

MAb

Mouse

Purified

IgG1,lAnti Mullerian hormone (AMH)
AMH
AMH
AMH
AMH
AMH
AMH
AMH
AMH
AMH
AMH Recombinant
AMH Recombinant
AMH Recombinant

9602
9603
E01347M
E01348M
E01350M
E01352M
E01353M
E01354M
E01355M
9604
R01712
R01713

MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb
Ag
Ag
Ag

Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Insect Cell
E. coliE. coliPurified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified

IgG1
IgG1
IgG2a
IgG1
IgG2a
IgG1
IgG2b
IgG3
IgG3
N/A
N/A
N/A

C-peptide
C-peptide
C-peptide
C-peptide
C-peptide
C-peptide

D24150R
E01336M
MHP18-601
MHP18-6014
MHP18-601CC

PAb
MAb
MAb
MAb
MAb

Rabbit
Mouse
Mouse
Mouse
Mouse

Neat
Purified
Purified
BiotinPurified

N/A
IgG1,k
IgG1
IgG1
IgG1

Calcitonin
Calcitonin
Calcitonin

E01356M
E01357M

MAb
MAb

Mouse
Mouse

Purified
Purified

IgG1
IgG1

Cortisol
Cortisol
Cortisol
Cortisol
Cortisol
Cortisol
Cortisol

E01332M
E45384M
E82294M
E86232M
E86322M
MAS01-501

MAb
MAb
MAb
MAb
MAb
MAb

Mouse
Mouse
Mouse
Mouse
Mouse
Mouse

Purified
Purified
Purified
Purified
Purified
Purified

IgG2a
IgG2b
IgG
IgG3
IgG1
IgG1,kDehydroepiandrosterone (DHEA)
DHEA

E01236M

MAb

Mouse

Purified

IgG2b

Estradiol
Estradiol
Estradiol (E2)
Estradiol-17 beta

MAS04-267
E01358M
E86022M

MAb
MAb
MAb

Mouse
Mouse
Mouse

Purified
Purified
Purified

IgG2a,kIgG1
IgG1

Estriol (E3)
Estriol
Estriol-6

E01360M
A82605P

MAb
Ag

Mouse
N/A

Purified
HRP

IgG1
N/A

www.meridianlifescience.com

30

Full product list continued
Specificity

Catalog #Type

A01283HAg

E45709M
E86123M

MAb
MAb

Human
Urine
Mouse
Mouse

E87701M

MAb

Mouse

H6F02-323

Ag

MAF02-145
MAF02-155
MAF02-243
MAF02-264
MAF02-275
MCF02-011
R01716

MAb
MAb
MAb
MAb
MAb
MAb
Ag

Human
Pituitary
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
CHO Cells

D01234G
D24460R

MAb
MAb

Goat
CultureHuman Chorionic Gonadotropin (hCG-b)
beta 98% Pure

A01278HAg

Human Chorionic Gonadotropin (hCG-a)alpha 98% Pure

A01280HAg

Human Chorionic Gonadotropin (hCG)
USP Grade

A01322HAg

Follicle-stimulating hormone (FSH)
Follicle-stimulating hormone (FSH)>98% Pure
Follicle-stimulating hormone (FSH), beta
Follicle-stimulating hormone (FSH-b), beta
Follicle-stimulating hormone (FSH) intact,
C1 Epitope
Follicle-stimulating hormone (FSH)>98% Pure
Follicle-stimulating hormone (FSH)
Follicle-stimulating hormone (FSH)
Follicle-stimulating hormone (FSH)
Follicle-stimulating hormone (FSH)
Follicle-stimulating hormone (FSH)
Follicle-stimulating hormone (FSH)
Follicle-stimulating hormone (FSH)
Glucagon
Glucagon (N-Terminal)
Glucagon-Like Peptide 1 (GLP1)

Host

FormatIsotype

Purified

N/A

Purified
Purified

IgG1
IgG

Purified

IgG1

Purified

N/A

Purified
Purified
Purified
Purified
Purified
Purified
Purified

IgG1,kIgG1,kIgG1,kIgG2a,kIgG
IgG1,kN/A

Purified
Purified

N/A
IgG1

Purified

N/A

Purified

N/A

Purified

N/A

Purified

N/A

Purified

N/A

Purified

N/A

Human Chorionic Gonadotropicn (hCG)

Human Chorionic Gonadotropin (hCG-b)
A01376H
beta 98% Pure
Human Chorionic Gonadotropin (hCG-a)
A01377H
alpha 95% Pure
Human Chorionic Gonadotropin (hCG)
A01378H
95% Pure
Human Chorionic Gonadotropin (hCG)
E01323M
Free beta
Human Chorionic Gonadotropin (hCG-b) beta E31586M
Human Chorionic Gonadotropin (hCG) alpha E86051M
Human Chorionic Gonadotropin (hCG-a) alpha E86082M
Human Chorionic Gonadotropin (hCG-b) beta E86308M
Human Chorionic Gonadotropin (hCG-b) beta E86820M
Human Chorionic Gonadotropin (hCG-b) beta MAF05-019
Human Chorionic Gonadotropin (hCG-b) beta MAF05-020
Human Chorionic Gonadotropin (hCG-b) beta MAF05-023
Human Chorionic Gonadotropin (hCG-b) beta MAF05-025
Human Chorionic Gonadotropin (hCG-b) beta MAF05-043
Human Chorionic Gonadotropin (hCG) beta MAF05-627
Human Chorionic Gonadotropin (hCG) beta MKF05-14831

Endocrine Disorders - Reagents for Assay Development

Ag
Ag
Ag

Human
Pregnancy
Urine
Human
Pregnancy
Urine
Human
Pregnancy
Urine
Human
Urine
Human
Urine
Human
Urine

MAb

Mouse

Purified

IgG1

MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb

Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse

Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified

IgG
IgG
IgG2b
IgG2a
IgG1
IgG1,kIgG1,K
IgG1,kIgG1,kIgG1,kIgG1,kIgG

Specificity

Catalog #

Type

Host

FormatIsotype

MAF06-154
MAF06-210
MAF06-212
MAF06-610
MCF06-610
E45600M
E86090M

MAb
MAb
MAb
MAb
MAb
MAb
MAb

Purified
Purified
Purified
Purified
Purified
Purified
Purified

IgG1,kIgG1,kIgG1,kIgG1,kIgG1,k
IgG1
IgG1

A75714H

Ag

Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Human
Pituitary

Purified

N/A

A01307HAg

E. coliPurified

N/A

Luteinizing hormone (LH)
Luteinizing hormone (LH), Bovine
Luteinizing hormone (LH)
Luteinizing hormone (LH)
Luteinizing hormone (LH)
Luteinizing hormone (LH)
Luteinizing hormone (LH)
Luteinizing hormone (LH) - beta
Luteinizing hormone (LH) - beta
Luteinizing hormone (LH) - beta
Luteinizing hormone (LH) 98% Pure
Luteinizing hormone (LH) Recombinant

D01237R
D10295G
MAF07-036
MAF07-037
MAF07-226
MAF07-412
E92120M
E92402M
E86124M
A01687H
R01715

PAb
PAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb

Rabbit
Goat
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Human
Mammalian
Cells

Neat
Neat
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified

N/A
N/A
IgG2a,kIgG1,kIgG1,kIgG1,k
IgG1
IgG1
IgG
N/A
N/A

Parathyroid Hormone (PTH)
Parathyroid Hormone (PTH) (a.a. 15-25)
Parathyroid Hormone (PTH) (a.a. 53-68)
Parathyroid Hormone (PTH) (a.a. 53-84)

E24170M
E24101M
E24150M

MAb
MAb
MAb

Mouse
Mouse
Mouse

Purified
Purified
Purified

IgG1
IgG1
IgG1

Ag

RetroplacenPurified
tal Blood

N/A

MAb

Mouse

Purified

IgG2a

MAb

Mouse

Purified

IgG2b

MAb

Mouse

Purified

IgG2b

MAb

Mouse

Purified

IgG2b

MAb

Mouse

Purified

IgG2a

MAb

Mouse

Purified

IgG2b

MAb

Mouse

Purified

IgG2a

Ag
MAb
MAb
MAb

N/A
Mouse
Mouse
Mouse

HRP
Purified
Purified
Purified

N/A
IgG1
IgG2b
IgG2b

Human growth hormone (hGH)
Human growth hormone (hGH)
Human growth hormone (hGH)
Human growth hormone (hGH)
Human growth hormone (hGH)
Human growth hormone (hGH)
Human growth hormone (hGH)
Human growth hormone (hGH)
Human growth hormone (hGH)
70% Pure
Human growth hormone (hGH),
Recombinant

Pregnancy associated plasma protein (PAPP-A)
Pregnancy associated plasma protein-A
A86864H(PAPP-A)
Pregnancy associated plasma protein-A
E01261M(PAPP-A)
Pregnancy associated plasma protein-A
E01367M(PAPP-A)
Pregnancy associated plasma protein-A
E01368M(PAPP-A)
Pregnancy associated plasma protein-A
E86114M(PAPP-A) proMBP subunit
Pregnancy associated plasma protein-A
E86141M(PAPP-A)
Pregnancy associated plasma protein-A
E86509M(PAPP-A) proMBP subunit
Pregnancy associated plasma protein-A
E86910M(PAPP-A)
Progesterone
Progesterone 3 – HRP Conjugate
Progesterone
Progesterone
Progesterone

A82606P
E82321M
E86222M
E86702M

Ag

www.meridianlifescience.com

32

Full product list continued
Specificity

Catalog #

Type

Host

FormatIsotype

Prolactin
Prolactin, Recombinant
Prolactin, Recombinant
Prolactin
Prolactin
Prolactin
Prolactin
Prolactin
Prolactin
Prolactin

A01467H
A01274H
E20122M
E20630M
MAF10-111
MAF10-145
MAF10-216
MAF10-245
MAF10-710

Ag
Ag
MAb
MAb
MAb
MAb
MAb
MAb
MAb

E. coliP. pastoris
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse

Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified

N/A
N/A
IgG1,kIgG1,kIgG1,kIgG1,kIgG2a,kIgG1,kIgG2a,kTestosterone
Testosterone
Testosterone
Testosterone 3
Testosterone 3, BSA Conjugate
Testosterone
Testosterone

E45703M
Y82230P
E82721M
A01312B
E86902M
E30412M

MAb
Ag
MAb
Ag
MAb
MAb

Mouse
Synthetic
Mouse
Synthetic
Mouse
Mouse

Purified
HRP
Purified
N/A
Purified
Purified

IgG1
N/A
IgG
N/A
IgG2a
IgG1

H6T08-747
E01326M
E01325M
A86852H

Ag
MAb
MAb
Ag

Human
Mouse
Mouse
Human

Purified
Purified
Purified
Purified

N/A
IgG2a
IgG2b
N/A

A01309H

Ag

Insect Cells

Aff.Pur.

N/A

E01309M
E01310M
E01311M
E86415M
H6T09-212

MAb
MAb
MAb
MAb
Ag

Purified
Purified
Purified
Purified
Purified

IgG1
IgG1
IgG1
IgG1
N/A

R01683

Ag

Mouse
Mouse
Mouse
Mouse
Sf9 cells
HEK293cells

Purified

N/A

D92409G
D01253P
G5T04-766
D01242G

PAb
PAb
PAb
PAb

Goat
Goat
Goat
Goat

Aff.Pur.
HRP
Aff.Pur.
Aff.Pur.

N/A
N/A
N/A
N/A

E20370M

MAb

Mouse

Purified

IgG1,k

H6T04-323

Ag

Purified

N/A

MAT04-001
MCT04-001
MAT04-003
MAT04-005
MAT04-006
MAT04-127
MAT04-252
MAT04-410
MDT04-005
E01328M
E01327M
E86107M

MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb

Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified

IgG1,kIgG1,kIgG1,kIgG1,kIgG1,kIgG1,kIgG1,kIgG1,kIgG1,k
IgG1
IgG1
IgG1

Thyroglobulin
Thyroglobulin
Thyroglobulin
Thyroglobulin
Thyroglobulin >90% Pure
Thyroid Peroxidase (TPO)
Thyroid Peroxidase (TPO) (a.a. 19-846)
RecombinantThyroid Peroxidase (TPO)
Thyroid Peroxidase (TPO)
Thyroid Peroxidase (TPO)
Thyroid Peroxidase (TPO)
Thyroid peroxidase (TPO), RecombinantThyroid peroxidase (TPO), RecombinantThyroid-stimulating hormone (TSH)
Thyroid-stimulating hormone (TSH) - beta
Thyroid-stimulating hormone (TSH) - beta
Thyroid-stimulating hormone (TSH) - beta
Thyroid-stimulating hormone (TSH) Intact
Thyroid Stimulating Hormone (TSH) alphaThyroid-stimulating hormone (TSH)
98% Pure
Thyroid-stimulating hormone (TSH)
Thyroid-stimulating hormone (TSH)
Thyroid-stimulating hormone (TSH)
Thyroid-stimulating hormone (TSH)
Thyroid-stimulating hormone (TSH)
Thyroid-stimulating hormone (TSH)
Thyroid-stimulating hormone (TSH)
Thyroid-stimulating hormone (TSH)
Thyroid-stimulating hormone (TSH)
Thyroid-stimulating hormone (TSH) - beta
Thyroid-stimulating hormone (TSH) Intact
Thyroid-stimulating hormone (TSH) Intact

33

Endocrine Disorders - Reagents for Assay Development

Human
Pituitary
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse

Specificity

Catalog #

Type

Host

FormatIsotypeThyroxine (T4)
Thyroxine (T4)
Thyroxine (T4)
Thyroxine (T4)
Thyroxine (T4)
Thyroxine (T4)
Thyroxine (T4)
Thyroxine (T4)
Thyroxine (T4)
Thyroxine (T4)

MAT02-011
MAT02-525
MAT02-641
MCT02-011
E45660M
MKT02-007
E86206B
E86206M
E86260M

MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb
MAb

Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse
Mouse

Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified
Purified

IgG1,kIgG1,kIgG1,kIgG1,k
IgG2b
IgG2b,k
IgG1
IgG1
IgG

Thyroxine binding globulin (TBG)
Thyroxine-binding globulin (TBG)
Thyroxine-binding globulin (TBG)

MAT03-439
MAT03-612

MAb
MAb

Mouse
Mouse

Purified
Purified

IgG2a,kIgG2a,kTriiodothyronine (T3)
Triiodothyronine (T3)
Triiodothyronine (T3)
Triiodothyronine (T3)

MAT01-237
E86207M
MAT01-121

MAb
MAb
MAb

Mouse
Mouse
Mouse

Purified
Purified
Purified

IgG1,k
IgG1
IgG1,kwww.meridianlifescience.com

34

Meridian Life Science, Inc.

5171 Wilfong Road | Memphis, TN 38134+1 901-382-8716
www.MeridianLifeScience.com
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08/21