Sperm Motility / Morphology Analysis System

SMAS

SMAS provides user friendly high-resolution
sperm motility and morphology analysis, with a 5 megapixel camera.
The original sperm identification algorithm will distinguishbetween motile and immotile spermatozoa.
SMAS is the only Japanese CASA system for measuring humanand animal sperm's motility, morphology and concentration.
Using SMAS will give you a great advantage in reducing
measurement errors during semen examination.

★

The microscope is optional.

Distinguishes between motile and immotile sperm The
system precisely measures total sperm count, concentration,and performes kinematic analysis in a short time.
In recent years, it has become clear that sperm morphology and motility constitute an important aspect of infertility.
The Sperm Motility / Morphology Analysis System (SMAS) automatically detects motile and immotile sperm and tracks
motile sperm.
It precisely measures the number of moving sperm, their orbit, and many other items in a short time.
Measerement results are displayed on-screen and can be presented in various output forms.
SMAS is the only CASA system produced in Japan.

C ha r ac t e r i st ics◀sperm tracking image* the yellow ” + ” indicates immotile sperm● Motile & immotile sperm and measurement results can be conﬁrmed in the image gained.
● With a high-resolution digital camera, the resolution is increased about four times compared to conventional products.
● About up to 5,000 motile sperms (a semen sample of about 400,000,000/ml concentration) can be measured in one ﬁeld of view.
● Result data and traced image will be saved automatically after analyzing.
● Original sperm image and tracking image can be saved also.
● The analysis result can be displayed according to WHO measurement standard (1999/2010) ABCD.
● Traces are color-coded according to sperm velocity to facilitate visualization.
● Progressive sperm analyzable.
● Custom category classiﬁcation / cut oﬀ ﬁlter available.
● Cost-less operation is possible by using a MAKLER counting chamber.

What can SMAS analyze?

countsconcentration

motilitymorphology
Major (Head) [μm]Oval (Head) [%]Motile number

Total
concentration

Motility rate

Perimeter (Head) [μm]Acrosome area [μm^2]
Midpiece width [μm]Midpiece position [μm]Bent (Tail) [deg]Immotile number

Minor (Head) [μm]Area (Head) [μm^2]

Motileconcentration
Total number

VSL/VCL/VAP/LIN/
STR/ALH/BCF/WOB

Roundness (Head) [%]Acrosome rate [%]Midpiece angle [deg]Length (Tail) [μm]LIN=VSL / VCL
STR=VSL / VAP

VCL

ALH
VAP
VSL

All hardware and software are developed by DITECT, a Japanese manufacturer.
We can offer ﬂexible services such as custom parameter setting and software upgrading.
Animal spermatozoa analysis for livestock reproduction is also available.

T r ack i n g a nd m easu rem en t
The operation is very simple. The user must only set semen
on a microscope and press the imaging button. The system
performs automatic processing from imaging tomeasurement and displays the result immediately. With an
original algorithm, the system distinguishes between motile
and immotile sperm. It tracks all behavior of moving sperm
for one second (standard) and calculates speciﬁed items.
Each sample can be measured as many times as desired inorder to correct the error of bias in the chamber.

Me asu r e me n t resu lt s
Immediately after imaging,the measurement results
are displayed on themonitor as numerical data
and visually. It is now
possible to improve theanalysis of individual
motility / morphology andto make a detailed
measurement of eachsperm.

An al y si s g raph view
Based on the individual sperm trace data obtained, thesystem calculates count, concentration, and other analysis
items. In addition, the system reports the motility results as ahistogram and as WHO-compliant four - and three - stage
graphs.

R ep o r t i n g fun ct ion
The measurement analysis results can be printed as a report data sheet.
The report includes from 30 printing items, including name / age / andsub information. Also one traced image, two graphs plotting themeasurement result, and ﬁve sets of measurement results laid out in on
A4 paper format.

Adv an t a g e usin g SM A S●A reliable data from imaging• SMAS is a imaging based CASA system. Like no other technics, measurement data are calculated using

High resolution
5,300,000 pixel

the sperm image it self and not by a statistical data or human fuzziness.

●High resolution, wide view

• Our 5 mega high speed camera will give SMAS a 2,592 X 2048 pixel and 60 fps and 1 square mm
image ﬁeld for the measurement. It is x 4, x 16 particular data than the existing models.

Wide image size
more than
1 square mm

Measurement
performance• This allows you to measure up to 5000 motile sperms (a semen about 400 milion/ml )●Head identification• An advanced head identiﬁcation algorithm will allow SMAS to separate non-sperm particles
and improve the accuracy of immotile count.

●Running cost

• Using makler counting chamber will give a great cost performance than the dedicated chamber systems.

High frame rate
60 fpsW ha t d o y o u need f or SM A S ?

Analysis
software

ComputerMicroscope
Counting chamber

CameraMeasurement
results

English Windows 7 or 10. 64 bit OS, intel CPU recommended.

SMAS has a 5 mega pixel 60 fps (it goes up to 5000 fps) digital high speed camera.

Phase contrast with 10x negative contrast ﬁeld lens for motility.
Bright ﬁeld contrast with 60x or 100x lens for morphology.

Makler counting chamber or disposable chamber with 10~12μm is recommended for motility.
Giemsa dye method for morphology.

sperm
O pt i o n s●Measurement chamber
A Makler counting chamber isavailable.
At the customerʼs request, we
can also oﬀer other options,such as a 12μm disposablechamber.

●Microscope

The system uses a phase-contrast microscopefor SMAS motility. You need a bright-contrast
microscope for SMAS morphogy.
You can also use your own microscope if it
can be connected to the camera. Low-cost
microscopes are also available.

So f t w a r e f eatures
SMAS will help your reproduction analysis tasks. A user-friendly
interface gives you the opportunity to express your analysis of theresults creatively.

Immediate
OutputUserFriendlyColor
CodingClear
Graphics
Detailed
Information

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You can make a request for more information and other inquiries.

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