HIF1-alpha IHC Antibody
Rabbit Polyclonal
Antigen Affinity Purified

RefSeq ID

NP_001521.1

Catalog No.

IHC-00460

Uniprot ID

Q16665

Lot No.

IHC-00460-3

GeneID

3091

APPLICATIONSIHC, ICC-IF

SPECIES REACTIVITY

Human

PRESUMED REACTIVITYAMOUNT

Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Zebrafish,Chicken, Turkey, Goat, Bovine, Dog, Horse, Rabbit, Guinea pig_10141, Pig, Panda, Orangutan
and Monkey
100 µlCONCENTRATION

200 µg/ml

STORAGE/SHELF LIFE

2 - 8°C / 1 year from date of receipt

PHYSICAL STATE

LiquidBUFFER

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide

ISOTYPEIgG

ORIGINUSA

PRODUCTIONPROCEDURES

Antibody was affinity purified using an epitope specific to HIF1-alpha immobilized on solid
support.
The epitope recognized by IHC-00460 maps to a region between residues 775 and the Cterminus (residue 826) of human hypoxia-inducible factor 1, alpha subunit using thenumbering given in Swiss-Prot entry Q16665 (GeneID 3091).
Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280
nm of 1.4 equals 1.0 mg of IgG.

APPLICATIONSCentrifuge tube to remove product from lid. Optimal working dilutions should be determined
experimentally by the investigator. Prepare working dilution immediately before use.

APPLICATION NOTES

Immunohistochemistry

1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 for 20
minutes using a pressure cooker is recommended for FFPE tissue
sections.

Immunofluorescence(ICC)

1:100 - 1:500

Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.

IHC HUMAN CONTROLSIn some cases, the antibody may be diluted further than indicated.
HeLa Cells

IHC MOUSE CONTROLSSquamous Cell Carcinoma

ADDITIONAL INFO

https://www.fortislife.com/p/IHC-00460
Use the link above to view SDS, a current list of citations, and other product specific information.

This document certifies that this product has met all of the quality control standards defined by Bethyl Laboratories, Inc.
Michael Spencer, PhD
Phone: 800.338.9579 • Fax: 866.597.6105 • Web: www.fortislife.com
Orders: orders@fortislife.com • Support: technical@fortislife.com

Date: December 12, 2022

1For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for human or animal consumption. This product may not be resold or modified for resale without the prior written
approval of Bethyl Laboratories, Inc. The information provided in this data sheet is believed to be correct but does not purport to be all-inclusive and is intended to be used as a guide. Bethyl Laboratories, Inc. shall not beliable or responsible in any way for use of either this information or the material supplied. Disposal of hazardous material may be subject to federal, state or local laws or regulations.

HIF1-alpha IHC Antibody

Detection of human HIF1-alpha byimmunohistochemistry. Sample: FFPE section of renal cell
carcinoma. Antibody: Affinity purified rabbit anti-HIF1alpha antibody (IHC-00460-3) used at a dilution of 1:100.
Detection: DAB

IHC-00460

Detection of human HIF1-alpha byimmunohistochemistry. Sample: FFPE section of HepG2
cells treated with cobalt-chloride. Antibody: Affinity
purified rabbit anti-HIF1-alpha antibody (IHC-00460-3)
used at a dilution of 1:100. Detection: DAB

Detection of human HIF1-alpha by immunocytochemistry.
Sample: Formaldehyde-fixed asynchronous hypoxic HeLa
cells. Antibody: Affinity purified rabbit anti-HIF1-alpha(IHC-00460 lot 2) used at a dilution of 1:100. Detection:
Red fluorescence

Phone: 800.338.9579 • Fax: 866.597.6105 • Web: www.fortislife.com
Orders: orders@fortislife.com • Support: technical@fortislife.com

3For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for human or animal consumption. This product may not be resold or modified for resale without the prior written
approval of Bethyl Laboratories, Inc. The information provided in this data sheet is believed to be correct but does not purport to be all-inclusive and is intended to be used as a guide. Bethyl Laboratories, Inc. shall not beliable or responsible in any way for use of either this information or the material supplied. Disposal of hazardous material may be subject to federal, state or local laws or regulations.