Veterinary &Animal HealthMolecular and Immunoassay
solutions for infectious
disease testing

Veterinary medicine has transformed the health and welfare of livestock and companion
animals worldwide.
As the globalized trade of animals and their products increases, the threat of infectious disease impacting livestock healthand food supplies has also increased in addition to the emergence of zoonotic diseases. Some infectious diseases havehighly visible consequences, while others remain silent for weeks or months. Accurate and rapid diagnostic tests are
therefore an essential component to detect, control and eradicate such diseases.
With so many different species and diseases in veterinary heath, vet diagnostic tests rely on both immunoassay and moleculartechnologies. qPCR has become an indispensable component of routine veterinary diagnostics due to its speed, sensitivity andselectivity, and potential for multiplex analysis. However, there is a growing need for field-based point-of-care testing to enablea rapid diagnosis and prevent transmission, especially when animals live in flocks and herds. Transport of specimens from thefield to the laboratory can be a lengthy process that can delay critical decision-making and severely affect the quality of thesamples. By using lateral point-of-care devices or ambient temperature stable qPCR assays, the need for transporting specimens
is reduced as assays can be performed on-site in low-resource settings such as farms and in the wild.

Optimized qPCR & RT-qPCR Mixes for Whole Blood, Urine and other Inhibitor-Rich Clinical Samples
High qPCR Reaction Efficiencies Across a Range of Crude Sample Types
Reaction efficiency (%)

100

Inhibitor-Tolerant qPCR Mix (MDX013)
was used in a series of qPCR reactions
containing a variety of crude clinicalsamples including whole blood,
cerebrospinal fluid (20%) and urine(20%). The results demonstrate thatthe reaction efficiency of the InhibitorTolerant qPCR Mix remained within
90-110% in the presence of a wide
range of common PCR inhibitors.

80
60
40
20

www.MeridianLifeScience.com

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Types of qPCR/RT-qPCR Optimized Mixes

Inhibitor-Tolerant
Mixes

Fast
Mixes

Lyo-ready
Mixes

Air-Dryable
Mixes

Ideal for multiplex
assays on fast,
automated highthroughput systems

Pre-formulated withlyo-excipients forlyophilization into
beads or cakes

Ready-to-use mix
compatible with a rangeof air-drying protocols
to produce an ambient
temperature stable assay

MDX013
MDX016

MDX020
MDX032

MDX021
MDX023
MDX024

MDX082
MDX092
MDX095

4x

2x

2x

4x

Hot-Start

Antibody

Antibody

Antibody

Antibody

AmbientTemperature
Assays---Optimized for extractionfree assays from crude
clinical specimens
Features(sputum, saliva and stool)Cat#

Concentration
Master Mix

Multiplex
Reactions--

Inhibitor-Tolerant--

RT-qPCR Control

RNA/DNA
Extraction-Free
Protocols

RT-qPCR
Extraction Control• Monitors assay inhibition• Suitable for use with
inhibitor-rich samples

MDX028 (Red, Quasar 670)
MDX029 (Orange,
Cal Fluor Orange)

VLP RNA Extraction Control• Closely mimics the test sample• Undergoes the same processing from lysis and extraction to detection• Compatible with lyophilization for creating freeze-dried mixes

MDX068 (Red, Cy5)
MDX069 (Orange, HEX)
MDX071 (Custom)

Recommended

www.MeridianLifeScience.com

Enzymes

LAMP

Ambient-Temp Stable
qPCR/RT-qPCR

MDX030ChemicalAntibodyAmAs biesa ntys Te
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RT-qPCRqPCR• Suitable•• Recommendedix

Molecular Product Selection Chart

••

••

••
••

Low DNA qPCR Mix
Fast qPCR Mix

MDX020Hi-Throughput dUTP Mix

MDX031 &MDX060Inhibitor-Tolerant qPCR Mix

MDX013

Inhibitor-Tolerant RT-qPCR Mix

MDX016

Fast 1-Step RT-qPCR Mix

MDX032

Low LOD 1-Step RT-qPCR Mix

MDX025

Lyo-Compatible MMLV-RT

MDX042

No

RNase-Tolerant MMLV-RT

MDX043No

MMLV-RT

MDX044

No

Air-Dryable™ qPCR Mix

MDX082

Air-Dryable™ 1-Step RT-qPCR Mix

MDX095

Air-Dryable™ Direct DNA qPCR Blood

MDX092

Lyo-Ready™ qPCR Mix

MDX021 &MDX023

Lyo-Ready™ 1-Step RT-qPCR Mix

MDX024

Lyo-Ready™ 1-Step RT-qPCR Virus Mix

MDX062

Lyo-Ready™ LAMP Mix

MDX097

Lyo-Ready™ RT-LAMP 1-Step Mix

MDX108Bst DNA Polymerase

MDX012

No

High Conc. Glycerol-Free Bst

MDX018No

Taq DNA Polymerase

MDX001

No

•

•

•

Taq HS DNA Polymerase

MDX008Antibody

••

••

••

Glycerol-Free Taq HS

MDX011

Antibody

••

••

•

Aptamer Taq HS (Glycerol-Free)

MDX015

Aptamer

••

••

••

Low DNA Taq HS

MDX009 &MDX010ChemicalHigh-Fidelity Pfu

MDX003No••

High-Specificity Pfu HS

MDX006

Aptamer••

Antibody••••

Antibody

•

••
••

••

••

••

••

••

••

••
••

••

Antibody••

Antibody••

Antibody

••

•

•
•

•

•

••








Antibody

••

••

••

Antibody

••

••

••

•

•

Antibody

••

••

••

••

•

Antibody

••

••

••

Antibody

••

•

••

Antibody

••

•

••




No

••

••

••

••

No

••

••

••

••

••

••

••

•

•

••

••

•

••

••

••

••

••

www.MeridianLifeScience.com

Optimized Mixes
PRODUCT

CAT NO.

VOLUME

REACTIONS

5 mL

500 Rxn

100 mL

10,000 Rxn

5 mL

500 Rxn

100 mL

10,000 Rxn

2 mL

500 Rxn

40 mL

10,000 Rxn

2 mL

500 Rxn

40 mL

10,000 Rxn

5 mL

500 Rxn

100 mL

10,000 Rxn

5 mL

500 Rxn

100 mL

10,000 Rxn

2 mL

500 Rxn

40 mL

10,000 Rxn

10 mL

1,000 Rxn

100 mL

10,000 Rxn

1 mL

200 Rxn

50 mL

10,000 Rxn

10 mL

1,000 Rxn

100 mL

10,000 Rxn

qPCR (DNA Template) Hot-Start Master Mixes
Low DNA qPCR Mix
Ideal for microbial and fungal DNA detection assays.

MDX030

Fast qPCR Mix
Suitable for fast high-multiplex qPCR assays.

MDX020

Fast qPCR Mix, 5x
Suitable for fast high-multiplex qPCR assays (5x concentration).

MDX072

Digital PCR Mix, 5x
Suitable for digital PCR (dPCR) assays (5x concentration).

MDX074Hi-throughput dUTP qPCR Mix
Optimized formulation for efficient dUTP incorporation to remove background PCR
product contamination.

MDX031

Inhibitor-Tolerant qPCR Mix
Designed for amplification direct from crude lysates or inhibitor-rich samples
such as urine, cerebral spinal fluid (CSF), blood as well as plants.

MDX013

Inhibitor-Tolerant qPCR Mix, 5x
Designed for amplification direct from crude lysates or inhibitor-rich samples such as urine,
cerebral spinal fluid (CSF), blood as well as plants (5x concentration).

MDX073

RT-qPCR (RNA Template) Hot-Start Master Mixes
Fast 1-Step RT-qPCR Mix
RT-qPCR mix formulated for fast, automated, high-throughput systems.

MDX032

Inhibitor-Tolerant RT-qPCR Mix, 4x
All-in-one, 4x master mix designed for RT-qPCR from crude samples such as sputum, stool
and saliva. No sample extraction required.

MDX016

Low LOD 1-Step RT-qPCR Mix
Suitable for detecting RNA and DNA viruses at very low levels for applications such as bloodbank or transplant viral testing.

MDX025

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Optimized Mixes
PRODUCT

CAT NO.

VOLUME

REACTIONS

5 mL

1,000 Rxn

50 mL

10,000 Rxn

5 mL

1,000 Rxn

50 mL

10,000 Rxn

5 mL

1,000 Rxn

50 mL

10,000 Rxn

5 mL

500 Rxn

100 mL

10,000 Rxn

8 mL

1,000 Rxn

100 mL

12,500 Rxn

10 mL

1,000 Rxn

100 mL

10,000 Rxn

10 mL

1,000 Rxn

100 mL

10,000 Rxn

5 mL

800 Rxn

50 mL

8,000 Rxn

5 mL

800 Rxn

50 mL

8,000 Rxn

Ambient-Temperature Stable qPCR/RT-qPCR Hot-Start Master Mixes
Air-Dryable™ qPCR Mix, 4x
Ready-to-use qPCR mix ideal for oven or air-drying technologies.

MDX082

Air-Dryable™ RT-qPCR Mix, 4x
Ready-to-use RT-qPCR Mix ideal for oven or air drying technologies.

MDX095

Air-Dryable Direct DNA qPCR Blood, 4x
Inhibitor-tolerant qPCR mix ideal for oven or air-drying technologies. Can be used for direct
detection using whole blood, serum or plasma samples.

MDX092

Lyo-Ready™ qPCR Mix
Ready-to-use, glycerol-free qPCR master mix formulated with a specialized
blend of excipients for lyophilization into beads or pellets.

MDX021

Lyo-Ready™ qPCR Mix, 2.6x
Ready-to-use, glycerol-free qPCR master mix formulated with a specialized
blend of excipients for lyophilization into beads or pellets (2.6x concentration).

MDX023

Lyo-Ready™ 1-Step RT-qPCR Mix
Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized
blend of excipients for lyophilization into beads or pellets.

MDX024

Lyo-Ready™ 1-Step RT-qPCR Virus Mix
Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipientsand highly suited for amplification of RNA viruses with a high secondary structure.

MDX062™

Loop-Mediated Isothermal Amplification (LAMP) Master Mixes
Lyo-Ready™ LAMP Mix
Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of
excipients for lyophilization into beads or pellets.

MDX097

Lyo-Ready™ RT-LAMP 1-Step Mix
Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of
excipients for lyophilization into beads or pellets.

MDX108

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Polymerases
PRODUCT

CAT NO.

VOLUME

REACTIONS

100 µL500 Units

10 mL

50,000 Units

10 mL

50,000 Units*

100 µL500 Units

10 mL

50,000 Units

10 mL

50,000 Units*

20 µL

1,000 Units

500 µL

25,000 Units

20 µL

1,000 Units

500 µL50,000 Units

100 µL500 Units

10 mL

50,000 Units

50 µL

500 Units

5 mL

50,000 Units

200 µL500 Units

10 mL

25,000 Units

5 mL

200 Rxn

100 mL

4,000 Rxn

1 mL

-

20 mL

-

8 µL

-

80 µL

-

2 mL

-

100 mL-DNA Polymerases
Taq DNA Polymerase
DNA Polymerase provided with optimized buffer system for fast PCR reactions across a rangeof templates. (*Enzyme only)

Taq HS DNA Polymerase
Antibody-mediated hot-start enzyme ideal for fast multiplex reactions. Robust performancewith low-copy number targets even in the presence of PCR inhibitors. (*Enzyme only)
Glycerol-Free Taq HS 50 U/µL
Lyophilization-compatible, high concentration (50 U/µL), glycerol free DNA enzyme
for automated high-throughput testing (provided as separate antibody, enzyme and dilution
buffer).

MDX001

MDX008MDX011

Aptamer Taq HS (Glycerol-Free) 50 U/μL
A high concentration, lyophilization-compatible Taq DNA polymerase containing a DNA
aptamer which binds reversibly to the polymerase. Suitable for developing highly specific,
high-throughput assays.

MDX015

Low DNA Taq HS 5 U/µL
Heat-activated, thermostable DNA polymerase suited amplificationof bacterial and fungal DNA.

MDX009Low DNA Taq HS 10 U/µL
Heat-activated, thermostable DNA polymerase suited to amplificationof bacterial and fungal DNA.

MDX010High-Fidelity Polymerases
High-Fidelity Pfu
3’ - 5’ proofreading exonuclease activity with an error rate of 3.0 x 10-6 and generates
blunt-ended amplicons up to 5 kb in length.

MDX003High-Specificity Pfu HS Mix
High-fidelity, aptamer-based hot start DNA polymerase tailored for low GC bias amplification.

MDX006Isothermal Polymerases
Bst DNA Polymerase
Stable, heat resistant DNA polymerase optimized for LAMP and contains
5´ > 3´ DNA polymerase activity and strong strand displacement activity.

MDX012High Conc. Glycerol-Free Bst
Lyophilization-compatible DNA polymerase for isothermal applications.

MDX018Inhibitor-Tolerant Bst Buffer
Optimized buffer for MDX012 and MDX018 that increases the amplification speed, sensitivity,
and tolerance to salt and other inhibitors found in clinical samples and transport media.

MDX019www.MeridianLifeScience.com

Polymerases
PRODUCT

CAT NO.

VOLUME

REACTIONS

8 µL

1,000 Rxn

80 µL

10,000 Rxn

200 µL

1,000 Rxn

2 mL

10,000 Rxn

200 µL

1,000 Rxn

2 mL

10,000 Rxn

50 mL

-

500 mL-

Reverse Transcriptase
Lyo-compatible MMLV-RT
High-concentration MMLV-RT suitable for incorporation into lyophilized RT-PCR assays.

MDX042

RNase-Tolerant MMLV-RT
Mixture of MMLV-RT and RNase inhibitor ideal for RT-qPCR assays.

MDX043

MMLV-RT
MMLV-RT suitable for generating first strand cDNA of up to 9 kb.

MDX044Next-Generation Sequencing
NGS Clean and Select Beads
Paramagnetic SPRI beads designed for clean-up and size selection of DNA fragments or
NGS libraries.

MDX041NGS ER Enzyme Mix
A mix that processes both 3’ and 5’ overhangs generating product that is 5’ phosphorylatedwith 3’ A overhang.

MDX0406 mL1,000 Rxn

NGS Ligase
Enzyme that catalyzes the ligation of adaptors to 3’ A overhangs.

MDX0372 mL

1,000 Rxn

NGS Library Quantification
qPCR-based assay for the quantification of only adapter-ligated library molecules.

MDX0391 Kit

500 Rxn

CAT NO.

VOLUME

REACTIONSNGS High-Fidelity Pfu Buffer, 10x
Optimized for use with High-Fidelity Pfu (MDX003).

MDX0385 mL

1,000 Rxn

NGS End-Repair Buffer, 5x
Optimized for use with NGS ER Enzyme Mix (MDX040).

MDX03510 mL

1,000 Rxn

NGS Ligase Buffer, 5x
Optimized for use with NGS Ligase (MDX037).

MDX0363 mL

1,000 Rxn

Taq PCR Buffer, 5x
Optimized for use with Taq DNA Polymerase (MDX001) and Taq HS DNA Polymerase(MDX008).

MDX0022 mL

500 Rxn

Bst Reaction Buffer, 10x
Optimized for use with Bst DNA Polymerase (MDX012) and High Conc.
Glycerol-Free Bst (MDX018).

5 mL

-

MDX076

10 mL-

Enzyme Dilution Buffer, 1x
A glycerol containing 1x dilution buffer, for the dilution of enzymes to reaction concentration.

MDX0785 mL-

100 mL-

Components
PRODUCT

Buffers

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Components
PRODUCT

CAT NO.

Enzyme Dilution Buffer (10x) Glycerol freeA glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration.

MDX080Tissue Extract-PCR Buffers
Lysis and neutralization buffer optimized for use with Taq HS DNA Polymerase (MDX008) toperform PCR direct from crude lysate.

MDX004

Fast qPCR Buffer, 4x
Optimized for use with Taq HS DNA Polymerase (MDX008).

MDX033

1-Step RT-qPCR Buffer, 4x
Optimized for use with Taq HS DNA Polymerase (MDX008) and RNase-Tolerant MMLV-RT(MDX043).

MDX034

Lyo-Ready™ qPCR Buffer, 2.5x
Optimized for use with Glycerol-Free Taq HS (MDX011). In order to produce lyophilized, ambienttemperature stable qPCR reagents, dNTPs, MgCl2 and glycerol-free Taq DNA polymerase are notincluded in the buffer.

MDX022

Lyo-Ready™ qPCR Buffer w/o Excipients, 4x
Optimized for use with Glycerol-Free Taq HS (MDX011).

MDX061

Taq Dilution BufferThis Buffer provides the optimal conditions to store Meridian polymerases, conferring long-term
stability at -20 °C (MDX008).

MDX007

Inhibitor-Tolerant PCR Buffer, 5x
Used with Taq HS DNA Polymerase (MDX008) for amplification direct from crude lysates orinhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants.

MDX075

Inhibitor-Tolerant Bst Buffer
Optimized buffer for MDX012 and MDX018 that increases the amplification speed, sensitivity,
and tolerance to salt and other inhibitors found in clinical samples and transport media.

MDX019VOLUME

REACTIONS

5 mL

-

10 mL

-

1 Kit

1,000 Rxn

2.5 mL

500 Rxn

50 mL

10,000 Rxn

2.5 mL

500 Rxn

50 mL

10,000 Rxn

8 mL

1,000 Rxn

100 mL

12,500 Rxn

2.5 mL

500 Rxn

100 mL

20,000 Rxn

5 mL

-

100 mL-

2 mL

500 Rxn

100 mL

20,000 Rxn

2 mL

-

100 mL-

General Reagents
Uracil DNA Glycosylase (UDG)#
Enzyme that efficiently hydrolyzes uracil from ssDNA or dsDNA. Endonuclease, exonuclease,nickase and RNase-free.

MDX05410 mL

10,000 Units

Proteinase K Solution
RNase and DNase free, ideal for removing endogenous nucleases when purifying native DNA or RNA.

MDX05525 mL

500 mgRNase Inhibitor
Inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and
C to control for contaminants in RT-PCR assays.

MDX056250 µL

10,000 Units

2.5 mL

100,000 Units

Taq HS Antibody
A mix of anti-Taq antibodies designed to inhibit Taq DNA polymerase activity
at room temperature. For use in hot-start PCR.

MDX01420 µL

1,000 Units

250 µL

12,500 Units#

Not available in the US* Not available in China

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dNTPsReady-to-use sequencing grade dNTPs available individually, as sets, or pre-blendeddNTP mixes. Ultra-pure (>99% by HPLC) free of PCR inhibitors, DNase and RNase.

PRODUCT

CAT NO.

VOLUMEdNTPs Lithium Salt
dNTP Set, 100 mM
Provided as four separate solutions at 100 mM concentration (dATP, dCTP, dGTP & dTTP).

MDX0504 x 250 µL
4 x 50 mL
500 µLdNTP Mix, 100 mM
Supplied as a mixture (dATP, dCTP, dGTP & dTTP). Total concentration of mix is 100 mM(25 mM of each nucleotide).

MDX05150 mL
100 mL
1L

dUTP Mix, 50 mM
Supplied as a mixture (10 mM dATP, dCTP, dGTP and 20 mM dUTP).

MDX05850 mL

dUTP, 100 mM

MDX05950 mL

dATP, 100 mM

MDX04650 mL

dCTP, 100 mM

MDX04750 mL

dGTP, 100 mM

MDX04850 mL

dTTP, 100 mM

MDX04950 mL

dNTP Mix, 10 mM sodium salt*

MDX067

Please EnquiredNTP Mix, 40 mM sodium salt*

MDX083

Please EnquiredNTP Mix, 100 mM sodium salt*

MDX084

Please EnquiredATP Mix, 100 mM sodium salt*

MDX063

Please EnquiredCTP Mix, 100 mM sodium salt*

MDX064

Please EnquiredGTP Mix, 100 mM sodium salt*

MDX065

Please EnquiredTTP Mix, 100 mM sodium salt*

MDX066

Please EnquiredNTPs Sodium Salt

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qPCR & RT-qPCR Extraction Controls

Designed for validating the steps within a qPCR or RT-qPCR
assay to produce more reliable and accurate results.

qPCR
The qPCR Extraction Controls are E. coli cells of a known concentration containing the Internal Control DNA sequence (with no known homology to
any organism, so that it does not interfere with the detection of the sample DNA). Genetic material from the test sample and the DNA extraction
control are simultaneously extracted by common extraction methods with the control being as sensitive to the same inhibition and extraction
failure as the test sample.

Product Highlights•	Extraction control undergoes the same sample processing
as test sample•	Confirms successes of extraction step and monitors
co-purification of PCR inhibitor inhibitors•	Can be used with a variety of sample types including
inhibitor-rich samples such as blood, urine and sputum• Probe based designed specifically for multiplex qPCR assays

PRODUCT• Control DNA is unique with no homology to any organism

CAT NO.

VOLUME

REACTIONSMDX02610 mL

2,000 Rxn

qPCR Extraction Control ORANGE (Cal Fluor® 560) MDX027

10 mL

2,000 Rxn

qPCR Extraction Control RED (Quasar 670)®

RT-qPCR
VLP-RNA Extraction Control is RNA molecule without any known homology, protected in a protein nanocage, harnessing the stability of viralstructures. VLP-RNA Extraction Control is spiked into the biological sample and undergoes the entirety of the sample processing, enablingusers of RT-qPCR assay to not only validate the success of the extraction and the reverse transcription steps, but also to determine thepresence of PCR inhibitors, reducing the chance of obtaining false negatives and resulting in reliable and accurate results. The VLP-RNA
Extraction Control is provided with its own detection system and can have its sequence customized if necessary.

Product Highlights•	Contains a defined number of copies of target RNA
molecules, encapsidated within a virus-like particle (VLP)•	Closely mimics the test sample, undergoing the same processing
route from lysis and extraction to RT-qPCR detection•	RNA sequence is customizable up to 1000nt•	Non-infectious material for ease of handling and shipping•	Compatible with commonly used RNA extraction methods
and lyophilization

PRODUCT

CAT NO.

REACTIONSVLP-RNA Extraction Control Red

MDX068VLP-RNA Extraction Control Orange

MDX069VLP-RNA Extraction Control

MDX071

1 mL (~1x10 4 copies/µL)
20 mL (~1x10 4 copies/µL)
1 mL (~1x10 4 copies/µL)
20 mL (~1x10 4 copies/µL)
Please enquire

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Antigens & Antibodies for Immunoassays
DESCRIPTIONTYPEAPPLICATIONCAT #Brucella abortus

DESCRIPTIONTYPEAPPLICATIONCAT #African Swine Fever Virus (ASFV)Intact Cells

MAb

ELISA, WB

C86116M

p30 Protein

MAb

ELISA, IFA, IHC, IP, WB C01881M

Intact Cells

MAb

ELISA, WB

C86131M

p30 Protein

MAb

ELISA, IFA, IHC, IP, WB C01882M

Coronavirus, Bovine

p30 Protein

MAb

ELISA, LF, Pair

9609

Surface Antigen(Peplomer)

p30 Protein

MAb

ELISA, LF, Pair

9610

p62 Protein

Ag (Rec.)

ELISA, WB

9605

p30 Protein

Ag (Rec.)

ELISA

R01793p30 Protein

Ag (Rec.)

ELISA

R01795

p54 Protein

Ag (Rec.)

ELISA

R01796

G Protein

MAb

ELISA, pair

C01974M

G Protein

MAb

ELISA, pair

C01975M

G Protein(Malaysia strain)

Ag (Rec.)

ELISA, pair

R01766ELISA, HIA

C86540M

PAb (Goat) ELISA, IFA

B65651G

MAb

Cryptosporisium, Bovine
Intact Oocysts

Foot-and-mouth disease (FMD)
Non-Structural
Protein (NS)

MAb

ELISA

C01452M

Serotype O1

MAb

ELISA

C01448M

Nebraska Calf Diarrhea Virus (NCDV, Rotavirus)
ELISA

C11222M

NCDV p43 (vp6)

MAb

NCDV (ICPs)

PAb (Goat) IFA

B65110G

NCDV (ICPs)

PAb (Goat) ICC

B65213G

NCDV (ICPs)

PAb (Goat) IFA

B65211G

NCDV (ICPs)

PAb (Goat) IFA

B65212G

Low Density Lipoprotein (LDL)
Ligand binding
region within
repeat #1

MAb

Nipah Virus (NiV)

Porcine Epidemic Diarrhea Virus (PEDV)
PEDV

MAb

ELISA, LF, WB, pair

C02005M

PEDV

MAb

ELISA, LF, WB, pair

C02006M

Nucleoprotein

Ag (Rec.)

ELISA, LF, WB

R01778Transmissible Gastroenteritis Virus (TGV)
IFA, WB, FC

H44070M

ELISA, IHC, WB

D01237R

Peplomer Protein

MAb

ELISA, Neut

C55802M

Peplomer Protein

MAb

ELISA

C55803M

Luteinizing Hormone (LH)
LH (X-reacts withsheep, goat and
deer)

PAb
(Rabbit)Trichomonas foetus
T. foetus

MAb

ELISA, IFA, pair

C01573M

T. foetus

MAb

ELISA, IFA, pair

C01572M

ELISA, LF, WB

R01801

Bovine Viral Diarrhoea Virus
BVDV-Erns EP
Glycoprotein

Ag (Rec.)

www.MeridianLifeScience.com

Antigens & Antibodies for Immunoassays
Description

Type

Application

Cat #Description

Type

Application

CAT #Parvovirus

MAb

ELISA, LF

C02003M

Parvovirus

MAb

ELISA, LF

C02004M

Parvovirus

MAb

ELISA, LF, pair

C86004M

Parvovirus

MAb

ELISA, pair

C86005M

Parvovirus
Canine Distemper Virus (CDV)
Surface Envelope
Antigen

MAb

ELISA, LF, IFA, pair

C86504M

Surface Envelope
Antigen

MAb

ELISA, IFA, pair

C86801M

Parvovirus

Ag (Native) ELISA

A01727CCDV

MAb

ELISA, LF, pair

C01986M

VP2 Protein

Ag (Rec.)

R01770

CDV

MAb

ELISA, LF, pair

C01987M

pro-Brain Natriuretic Peptide, N-Terminal (NT-proBNP)

CDV

MAb

ELISA, LF, pair

C01988M

CDV

MAb

ELISA, LF, pair

C01989M

N-Terminal, a.a.
40-50

MAb

ELISA

H01353M

CDV

MAb

ELISA, LF, pair

C01990M

MAb

ELISA

H01354M

CDV

Ag (Native) ELISA

A01726C

N-Terminal, a.a.
64-72

Coronavirus

Goat

ELISA, IEP, pair

C02011G

Coronavirus

MAb

ELISA, pair

C01998M

Coronavirus

MAb

ELISA, pair

C01999M

Nucleoprotein

MAb

ELISA, pair

C02000M

Nucleoprotein

MAb

ELISA, pair

C02009M

Nucleocapsid

MAb

ELISA, LF, WB, pair

C02010M

Coronavirus

Ag (Native) ELISA

A01730N

CRP

MAb

ELISA, pair

M01345M

CRP

MAb

ELISA, WB

M01346M

CRP

MAb

ELISA, pair

M01347M

CRP

MAb

ELISA, pair

M01348M

CRP

Ag (Rec.)

ELISA, pair

R01772Cystatin C

MAb

ELISA, pair

H01303M

Cystatin C

MAb

ELISA, pair

H01304M

Coronavirus

C-Reactive Protein

Cystatin C

ELISA, pair

Serum Amyloid A (SAA)
SAA

MAb

ELISA, WB, pair

H01381MSAA

MAb

ELISA, WB, pair

H01383M

SAA

MAb

ELISA, WB, pair

H01384M

SAA

MAb

ELISA, WB, pair

H01382MSAA

MAb

ELISA, WB, pairH01425M

SAA

MAb

ELISA, WB, pairH01426MSAA

MAb

ELISA, WB, pairH01427MSAA

MAb

ELISA, WB, pairH01429M

SAA

MAb

ELISA, WB, pairH01430M

SAA

Ag (Rec.)

ELISA, pair

R01771Thyroid Stimulating Hormone (TSH)
Beta subunit

MAb

ELISA, WB, pair

E01369M

Beta subunit

MAb

ELISA, WB, pair

E01370M

Thyroglobulin
Thyroglobulin

Ag (Native) ELISA

A01725C

Heartworm (Dirofilaria immitis)
D. immitis

MAb

ELISA, pair

C01991M

D. immitis

MAb

ELISA, pair

C01992M

D. immitis

MAb

ELISA, pair

C01993M

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Antigens & Antibodies for Immunoassays
Description

Type

Application

Cat #Cystatin C

Description

Type

Application

CAT #Infectious Bronchitis Virus (IBV)

Cystatin C

MAb

ELISA, pairH01431M

Nucleoprotein

Cystatin C

MAb

ELISA, pair

H01303M

Infectious Bursal Disease (IBDV)

Cystatin C

MAb

ELISA, pair

H86217M

VP2 Protein

MAb

ELISA, WB, pair

C01524M

VP2 Protein

MAb

ELISA, WB, pair

C01526M

Feline Immunodeficiency Virus (FIV)

MAb

ELISA, WB

C01530M

Core Antigen

Ag (Rec.)

ELISA, LF

R65360VP3 Protein

MAb

ELISA, WB, IHC, pair C01525M

FIV

Ag

ELISA

A01731FVP3 Protein

MAb

ELISA, WB, IHC, pair C01528M

Feline Leukemia Virus (FeLV)

Influenza A H5 (H1H5)

Glycoprotein 70(gp70)MAb

ELISA, IFA

C65712M

p27 Protein

MAb

ELISA, WB, pair

C01995M

p27 Protein

MAb

ELISA, WB, pair

C01996M

p27 Protein

PAb (Goat) WB

B65221G

p27 Protein

PAb (Goat) ELISA, IFA, WB

C01997G

p27 Protein (a.a.
272 - 519)

Ag (Rec.)

R01776ELISA, WB

Parvovirus
Parvovirus

Ag(Lysate)

ELISA

A01728F

Hemagglutinin H5

MAb

ELISA, DB, HIA, pair C86240M

Hemagglutinin H5

MAb

ELISA, pair

C01309M

Influenza A H7 (H1H7)
Hemagglutinin H7

MAb

ELISA, WB

C01610M

Hemagglutinin H7

MAb

ELISA, WB

C01611M

Marek Disease Virus
Serotypes 1, 2 & 3

MAb

ELISA

C01264M

Serotypes 1, 2 & 3

MAb

ELISA

C01267M

Serotypes 1, 2 & 3

MAb

ELISA

C01268M

Newcastle Disease Virus (NVD)

Serum Amyloid A (SAA)HN Glycoprotein

MAb

ELISA, HIA

C86012MSAA

MAb

ELISA, WB, pair

H01381M

HN Glycoprotein

MAb

ELISA, HIA

C86014M

SAA

MAb

ELISA, WB, pair

H01383M

HN Glycoprotein

MAb

ELISA, HIA, WB

C86016MSAA

MAb

ELISA, WB, pairH01425M

HN Glycoprotein

MAb

ELISA, HIA

C01351MSAA

MAb

ELISA, WB, pairH01426M

HN Glycoprotein

MAb

ELISA

C01352MSAA

MAb

ELISA, WB, pairH01427M

Ribonucleoprotein

MAb

ELISA, IFA, IHC

C01629MSAA

Ag (Rec.)

ELISA

R01774

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Antigens & Antibodies for Immunoassays
Description

Type

Application

Cat #Description

Type

Application

CAT #Suitable for use with dog, bovine, monkey and rat serum

Burkholderia (Pseudomonas) mallei
Whole cells

MAb

Indirect ELISA

C01752M

Adenovirus Hexon

LPS

MAb

ELISA, WB

C86315M

Hexon Antigen

MAb

ELISA, IHC, ID, pair

C86804M

Hexon Antigen of
1, 5, 8 & 27

MAb,
biclonal

ELISA, LF, pair

C86006M

Hexon Antigen of
1, 5, 8 & 27

MAb,
biclonal

ELISA, ID, pair

C86007M

C86420M

Cystatin C
Cystatin C

MAb

ELISA, pair

H01303M

Cystatin C

MAb

ELISA, pair

H86013M

Cystatin C

MAb

ELISA, pairH01431M

Serum Amyloid A (SAA)

Borrelia (Lyme Disease)

SAA

MAb

ELISA, WB, pair

H01381M

B. burgdorferi
garinii

MAb

ELISA, IFA, WB

SAA

MAb

ELISA, WB, pair

H01383M

B. afzelii VlsE

Ag (Rec.)

ELISA, LF, CLIA, WB R01609

SAA

MAb

ELISA, WB, pair

H01384M

SAA

MAb

ELISA, WB, pair

H01382M

ELISA, DB, WB

SAA

MAb

ELISA, WB, pairH01425M

B. burgdorferi VlsE,
multi-epitope
Ag (Rec.)chimeraSAA

MAb

ELISA, WB, pairH01426M

B. garinii p14
antigen (flagellin)

Ag (Rec.)

ELISA, LF, CLIA, BD,
R01521
WB

SAA

MAb

ELISA, WB, pairH01427M

B. garinii VlsE

Ag (Rec.)

ELISA, LF, CLIA, WB R01610

SAA

MAb

ELISA, WB, pairH01429M

Cryptosporidium parvum

SAA

MAb

ELISA, WB, pairH01430M

C. parvum

MAb

ELISA, LF, WB, pair

C02007MSAA

Ag (Rec.)

ELISA, pair

R01773

C. parvum

MAb

ELISA, LF, WB, pair

C02008M

MAb

Virus Neut., ELISA,
IHC, IFA

C86307M

R01523

RabiesRabies

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Recommended Blockers

Blockers for interference in sandwich immunoassays

Animal IgG – Passive Blockers• Suited for mixed species assays (e.g. MAb/PAb)•	Species of blocker must be the same as the host of thecapture or detection antibody

Mouse IgG

Goat IgG

A66186M (9-13mg/mL)

A66200H

A66185M (50-55mg/mL)
A66189M (45-55mg/mL, No Azide)
A66185M-LY (Lyophilized)

Passive blocking reagents work by preventing interfering
antibodies from binding to the capture or detection antibodies
by providing alternate binding sites. Animal IgG (e.g. Goat IgG)
can only block one type of interference (e.g. human anti-goatantibodies) so typically more than one type must be used,
depending on the host of both the capture and detection
antibodies. Animal IgG must be added in excess concentrationand the effectiveness depends on the affinity of interfering
antibody for the animal IgG.

Rat IgG
A64391R

Chicken IgY

Rabbit IgG

A01302C (Lyophilized)

A66100H

Sheep IgG

RECOMMENDED CONCENTRATION:•	10x the concentration of the MAb/PAb being used in
the assay (e.g. if 5μg/mL of Ab/conjugate, add 50μg/mL
Animal IgG).
•	Can be added to the sample or conjugate diluent but
ideally should be in contact with the patient sample
before incubation with the assay capture antibody.

A66400S (70-77mg/mL, Liquid)

TRU Block™ – Active HAMA & RF Blocker• Suited for double mouse monoclonal assays• Removes HAMA, HA & RF interference

TRU Block™ Ready8001
TRU Block

TRU Block™ Ultra
TRU Block8000
TRU Block™
A66800H

Interfering HA(e.g. HAMA)

IgM RFTRU Block™ 2
A66802H

Detection Antibody(mouse origin)

TRU Block™ 3
A66803H

Capture Antibody(mouse origin)

Antigen*Formulations differ in their ratio of various
proprietary ingredients that confers
unique blocking characteristics.

Blocking
BufferIn double mouse monoclonal assays, a specific blocker is required toremove a particular type of HA interference called human anti-mouse
antibodies (HAMA) and Rheumatoid Factor (RF). A HAMA blocker
contains a specific binder directed against all types of heterophilicinterference including HAMA and RF. Once bound to the interfering
antibodies, TRU Block prevents further binding of HA to other assay
components through steric hindrance. Active blockers can typicallybe used in lower concentrations than passive blocking reagents,
which minimizes the reduction in assay signal commonly associatedwith passive blockers.

RECOMMENDED CONCENTRATION:
For best performance, TRU Block should be included aspart of the sample or conjugate diluent, at a recommended
concentration range:
Product

Protein Concentration

Application

TRU Block
Ready

Single-step dilution withrecommended dilutionof 1:1000 to 1:10

ELISA & LF

TRU Block™
ULTRA

Range: 24-26 mg/mL

ELISA, CLIA & LF™

TRU Block™

Range: 24-26 mg/mL

ELISA & CLIA

TRU Block™ 2

Range: 24-26 mg/mL

CLIA & LF

TRU Block™ 3

24.3 mg/mL

ELISA

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PairsAdenovirus

C. parvum

CanineDistemper
Virus

Coronavirus

CRP

Cystatin C

CaptureAntibody

Detection
Antibody

CaptureAntibody

Detection
Antibody

C86804M

C86006M

C01524M

C01526M

C86006M

Infectious
Bursal Disease(IBDV)

C86007M

C01525M

C01528M

C02008M

C02007M

Nipah Virus

C01975M

C01974M

C01986M

C01988M

C02003M

C02004M

C86005M

C86004M

R01770C02006M

C02005M

R01778

H01381M

H01383M

H01426M

H01425M

R01771

H01426M

H01425M

R01773

H01426M

H01425M

R01773

Parvovirus

Antigen Application

R01766

C01987M

C01988M

C01990M

C01989M

C86504M

C86801M

C01998M

C01998M

C02010M

C02009M

C02009M

C20211G

C02010M

C20211G

C01999M

C01998M

M01347M

M01345M

R01772

H01427M

H01425M

R01771

M01348M

M01345M

R01772

H01427M

H01425M

R01773

H01303M

H86013M

H01427M

H01425M

R01774

H01304M

H01303M

H01429M

H01384M

R01771

H01431M

H01303M

H01429M

H01384M

R01773

H01431M

H86217M

H01429M

H01383M

R01771

H01429M

H01383M

R01773

H01384M

H01430M

R01771

H01384M

H01430M

R01773

H01382M

H01384M

Trichmonas
foetus

C01573M

C01572M

TSH

E01370M

E01369M

FelineLeukemia
Virus (FeLV)

C01996M

H1H5C01309M

C86240M

C01992M

C01992M

C01993M

C01992M

Heartworm

Antigen Application

CanineBovinePorcineEpidemic
Diarrhea Virus(PEDV)

Serum
Amyloid A(SAA)

C01995M

EquineAvian

FelineSwine

Multi-species

Ordering information:
USA
5171 Wilfong Road
Memphis, Tennessee 38134
Phone: +1 901-382-8716
Fax: +1 901-333-8223

Email: info@meridianlifescience.com
Orders: orders@meridianlifescience.com

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