IMTAKT HPLC COLUMN
3µm silica particles
13nm pore
Methylsilyl ligands (USP: L13)
pH 1.5-9

Highly durable C1 phase
Amazing durability for acidic mobile conditions using formic acid or TFA
Excellent for compounds with a wide range of polarity
Analysis of super hydrophobic compounds which are difficult to elute on an ODS phase
Alternative selectivity from C18 phase
High-performance, high pH 3µm particles

Acid stability (0.1% TFA)

Acid stability (0.5% HCOOH)
Traditional C1(butylbenzoate)

100
Nucleic bases and nucleosides
250 x 3 mm
0.5% HCOOH aq.
0.4 mL/min, 37°C, 260nm

80

0.1% TFA

6040Traditional C124hours

2024hours
0.5% HCOOH

0020406080

100120hours
0510min

0510min

A29Tizanidine (muscle-relaxant) impurity
ClNHNHNN

50 x 3 mm
A: 10mM HCOONH4
B: 50mM HCOONH4 / ACN= 80 / 20
0 - 10%B (0 - 10min)
0.4mL/min (5MPa), 37°C
2 µL (0.1mg/mL, DMSO)
320nm

NS

tizanidine

2468 minDurability of traditional C1 phases under aqueous
mobile phase conditions has always been asignificant challenge due to the ease of ligandhydrolysis. To address this problem, we utilized
stationary phase designs from our Scherzo andIntrada series resulting in extraordinary aqueous
stability for this C1.
Unison UK-C1, as expected, has lower
hydrophobic retention than a C18 column, but due
to proximity of polar silica surface, these columns
exhibit hydrophilic retention characteristics. This
provides a balance of both hydrophobic andhydrophilic separation modes, bringing differential
selectivity for individual compounds. In the figure
to the left, an impurity was resolved on our C1phase, but not on a C18, thanks to this alternativeselectivity.

Brand-XB C18

0Product Advantages

A27

Additionally, the Unison UK-C1 column easily
separates highly hydrophobic compounds, which
can be very difficult to elute on C18 columns.

A complementary stationary phase to C18 columns
Hydrophobic interactions are the main solute retention mechanism on C18 stationary phases. However, othermechanisms, such as electrostatic interactions with the base material, can also contribute. The resulting retentionof each solute is determined by the sum of all molecular interactions, with experimentally measured separation
between solutes manifested as the different ratios of individual interactions contribution at molecular level. C1phases have low hydrophobicity due to the only methyl groups (C1) on the surface, a stark contrast with highhydrophobicity from the long alkyl chains in C18s. However, the methyl group on C1s allows a long-range soluteinteractions with the polar groups on the base silica surface, providing a different selectivity.
A36

BASIC COMPOUNDSOHClNCH3

H3 C

eperisone (antispasmodic)

75 x 4.6 mm
A: water / formic acid= 100 / 0.1
B: acetonitrile / formic acid = 100 / 0.1
0-50%B (0-15min)
1 mL/min (7MPa), 37°C
5 µL (0.05mg/mL, DMSO), 260nm
051015 min

C18 columns often produce a broad peak when using
formic acid modified mobile phases, especially forbasic compound due to the difficulties in control ofamine dissociation. C1 phases may provide better peak
shape under the same conditions because they have awell-balanced mix of both hydrophobic andelectrostatic mechanisms that can improve separations.
Durability of the Unison UK-C1 column under formic
acid conditions has been dramatically improved with
its unique anti-hydrolysis surface structure.
When conventional C18 columns fail due to poor peak
shape under acidic conditions, our Unison UK-C1
column can be an excellent alternative to improveseparations.
CH3

CH3

CH3

H3C

CH3

TRITERPENS

CH3Terpenoids and triterpenes are very hydrophobic compounds,
typically requiring the use of non-aqueous conditions on C18columns to facilitate elution.

squaleneUnison UK-C1 column has an advantage for these and otherhighly hydrophobic compounds because it can be used witha variety of combinations of aqueous and organic solvents forelution and successful separation.

150 x 4.6 mm
A: acetonitrile / water = 70 / 30
B: acetonitrile
25-100%B (0-15min)
0.8mL/min (7MPa)
37°C, ELSD
1 µL (5 mg/mL,THF)

2
1

O

3

OO
OOO

1020 min

O

2
3

4

56

70-100%B (0-30min)

10

Quantification of phthalic acid esters can bechallenging because they have multiple branchedchain isomers, which can produce multiple peaks on
C18 columns, adversely impacting accuratequantification.

In such a case, the methyl group stationary phase ofUnison UK-C1, which show poor molecular
56
recognition for alkyl compounds, are an advantage
because it can coalesce multiple isomers into a single
diisodecyl phthalate peak, considerably simplifying data interpretation.
diisononyl phthalate
DIDP
DINP

15OO

C9H19OC9H19O0O

di-2-ethylhexyl phthalate di-n-octyl phthalate
DEHP
DNOP

51

dibutyl phthalate
DBP

O

50-100%B (0-20min)

0A35O

4O

615 min

10O

butylbenzyl phthalate
BBP

55OOO

34CH3O

2OCH3squalane

01CH3CH3CH3CH3Therefore, Unison UK-C1 may deliver excellent results for not
only hydrophilic but also hydrophobic compounds.

PHTHALIC ACID ESTERS

CH3H3C

2030 min

OC10H21OC10H21O

150 x 4.6 mm
Unison UK-C1 column will undoubtedly contribute to
A: water / AcOH = 100 / 0.1
this kind of branched chain isomers
B: acetonitrile / AcOH = 100 / 0.1 improve
0.8 mL/min (9MPa )quantification analysis.
37 °C, 260nm
2 µL (1 mg/mL, DMSO)
A34

PRODUCT INFORMATION 3 or 5 µm silica, 13nm pore, methyl group phase (USP: L13), 250bar MAX (HT: 500bar, UP: 1000bar)
COLUMN NAME

(3µm)
(5µm)

COLUMN ID

COLUMN LENGTH

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1mm, 1.5mm, 2mm, 3mm
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10mm, 20mm, 30mm
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