Cardiac &Metabolic Markers
Reagents for Assay Development
ISO Certified 13485:2016

www.MeridianLifeScience.com

Company Overview

Extensive Capabilities & Services
Molecular Reagents
qPCR | RT-qPCR | LAMPENZYMES

Immuno Reagents
Antigens | Antibodies | Blockers•	Hot-Start Taq technologies - chemical,
antibody, aptamer

VIRUS MANUFACTURING•	Lyo & Air-Dryable enzymes (glycerol free) Taq,
Bst, RTase•	Proprietary Ag purification•	Live or inactivated•	Thermostable MMLV RT

RECOMBINANT PROTEINSMASTER MIXES•	Lyo & Air-Dryable formats•	Inhibitor-tolerant mixes for stool, sputum, saliva,
blood, plant, water.
•	For multiplexing, GC-rich templates•	
E. coli, P. pastoris, S. cerevisiae, Sf9,
Mammalian (CHO, HEK293)•	10L- 130L fermentation

ANTIBODIES – MAbs/PAbs•	500+ MAbs produced in grams

II

NUCLEOTIDES•	Multi-Kilograms of MIgG / year•	dNTPs, Na or Li salts•	Hundreds of liters of GxhIgG•	Ultra high purity, >99%•	Ascites production (55,000 Mice)

Cardiac & Metabolic Markers for Assay Development

Commercial scale manufacturingof antigens and antibodies withprotein purification expertise.
Meridian has been providing innovative life science solutions and building
trusted partnerships for over 43 years. Meridian’s focus is to offer complete
solutions for the development of molecular and immunological assays.
• Full line of immunoassay reagents, including antigens, antibodies and blockers• Large scale production of reagents for molecular assays• Technical support with assay development experience• Dedicated R&D and manufacturing teams•R obust and mature Quality System

ISO Certified 13485:2016

Global presence
WATERLOO, Belgium
QUEBEC, Canada

LONDON, United Kingdom
LUCKENWALDE, Germany

BILLERICA, MA
MANASQUAN, NJ
CINCINNATI, OH (Headquarters)

BEIJING, China

PARIS, France

CHANGZHOU, China

MILAN, Italy
MEMPHIS, TN
BOCA RATON, FL

MODIIN, Israel

SYDNEY, Australia

Diagnostic Manufacturing |Life Science Manufacturing |Sales & Warehouse

MERIDIAN BIOSCIENCE, INC.
Parent Company | Founded in 1977 | Nasdaq: VIVO | 750+ Employees
Headquartered in Cincinnati, OH | Presence in 70+ Countries.

www.MeridianLifeScience.com

IIICompany Overview
Antigens & Antibodies
INFECTIOUS DISEASE EXPERTISE

IV

Tropical

ToRCH & Childhood

Viral Hepatitis•	Zika•	Dengue 1, 2, 3, 4
•	Chikungunya•	Malaria•	Chagas•	Leishmaniasis•	Leptospirosis•	Newcastle Disease•	Yellow Fever•	Nipah Virus•	JEV•	Toxo•	Rubella•	CMV•	HSV-1,2•	Rubeola•	EBV•	Mumps•	Coxsackie•	Rotavirus•	RSV•	Parvo B19•	VZV•	HAV•	HBV•	HCV•	HDV•	HEV

Cardiac & Metabolic Markers for Assay Development

STD•	HSV-1, 2•	HIV-1, 2•	HPV•	Syphilis•	Chlamydia•	Neisseria

Gastro•	
H. Pylori•	C. Difficile•	Norovirus•	Adenovirus•	Rotavirus•	Cryptosporidium•	Campylobacter•	
E. Coli•	Salmonella•	
G. Lambia•	Astrovirus

Respiratory•	SARS-CoV-2•	
M. Pneumoniae•	
C. Pneumoniae•	Influenza A, B
•	Parainfluenza•	
L. Pneumophilia•	RSV•	
M. Tuberculosis•	Streptococcus•	Staphylococcus•	Adenovirus

Cardiac

Cancer

Veterinary

Drug of Abuse•	Troponin I, T•	Myoglobin•	BNP•	NT-proBNP•	CRP•	PCT•	CK-MB•	D-Dimer•	Cystatin-C•	Galectin-3•	Vitamin D•	Apo A, B, E
•	NSE•	FABP•	SAH•	MPO•	Fibrinogen•	EGF•	Lp-PLA2•	PAPP-A•	CA125•	CA15-3•	CA19-9•	CA72-4•	CA50•	CA242•	Cyfra 21-1
•	CEA•	Thyroglobulin•	erbB-2/HER2•	AFP•	EGFR•	HE4•	NSE•	PMA•	PAP•	PSA•	PSMA•	S-100•	PIVKA II•	B2M•	ASFV•	Avian Influenza•	Borrelia•	
Brucella abortus•	Canine Distemper•	Feline
Immunodeficiency•	Feline Leukemia•	Foot-and-Mouth•	Canine Heartworm•	Infectious Bursal
Disease•	Marek Disease•	Newcastle Disease•	Canine Parvovirus•	Rabies Virus•	Serum Amyloid A (SAA)• Trichomonas foetus•	Nipah•	Transmissible
Gastroenteritis•	Amphetamine•	Barbital•	Benzodiazepine•	Buprenorphine•	Cocaine•	Cotinine•	EDDP•	Fentanyl•	Ketamine•	K2•	MDMA (Ecstasy)•	Methadone•	Methamphetamine•	Morphine•	Norketamine•	Opium•	Oxycodone•	PCP•	Phenobarbital•	Propoxyphene•	THC

Autoimmune

Microbial Detection•	Jo-1•	PCNA•	pANCA•	cANCA•	Sm Ag•	dsDNA•	La(SSA)•	Ro(SSA)•	Histone•	GMB•	C1q•	Scl-70•	SS-A•	BS-Gly-1•	Cathepsin G•	Calprotectin•	Legionella•	Salmonella•	Cryptosporidium•	
G. Lambia•	
C. Jejuni•	
E. Coli•	
B. Anthracis•	Clostridium•	Listeria•	Streptococcus•	Staphylococcus

Immunoglobulins/
Blockers

Hormones•	LH, FSH, hCG,
•	hGH, AMH•	Cortisol•	Estradiol•	Insulin, C-peptide•	Prolactin•	Progesterone•	PTH•	PAPP-A•	TSH, T3, T4, ACTH
•	Thyroglobulin

Allergens•	Cat & Dog Allergen•	Horse Allergen•	Dust Mite
• Alternaria alternate•	Timothy Grass•	
Platanus acerifolia•	Mugwort•	TRU Block™ &IgM Diluent•	Animal IgGs – Bovine,
Chicken, Goat, Mouse,
Rabbit, Sheep•	Human IgA, IgG,
IgM, IgE• Kappa Light chain• Lambda Light chain•	Goat Anti-Human
IgG, IgM, IgA•	Goat Anti-Mouse IgG

www.MeridianLifeScience.com

VCardiac & Metabolic Diagnostics
Cardiovascular and metabolic diseases are the leading cause ofdeath globally. An aggressive approach to their diagnosis andtreatment can substantially reduce the risk of mortality and lifethreatening complications.
Cardiovascular disease (CVD) is a major
health problem across the world, accounting
for a third of all deaths worldwide. It is oftengrouped with metabolic disorders because it
is frequently a consequence of diabetes anddyslipidemia. However the group of disorders
specifically related to CVD include coronary
heart disease, cerebrovascular disease, andperipheral arterial disease.
Cardiac biomarker assays have proven to
be particularly useful in providing a rapid
diagnosis and assessing risk in people withacute coronary syndrome (ACS). Cardiac
markers are proteins, hormones, enzymes orother substances expressed by myocardial
cells and they are released into the circulating
blood upon cell necrosis. Several biomarkers
that have become routinely used in the clinic
Retrieved 2013: www.cardiac-biomarkers.com
include creatine kinase (CK), creatine kinase-MB(CK-MB), myoglobin, cardiac troponin T (cTnT), and cardiac troponin I (cTnI). To date, the troponins have proven to be
the most sensitive and specific indicators of cardiac injury. Other useful biomarkers include NT-proBNP which canhelp predict an increased risk of recurrent events after a heart arythmia. New potential cardiac biomarkers arecontinually being researched, and advances in functional genomics, proteomics, metabolomics, andbioinformatics have revolutionized the discovery process. In general, using more than one biomarker increases theearly predictive value compared to using on a single marker and technological advances will increase the use ofmulti-marker profiling to improve and possibly individualize treatment of CVD in the future.
Metabolic diseases include disorders that disrupt normal metabolism such as diabetes and dyslipidemia (abnormal
lipid metabolism). These diseases can be brought on by primary (genetic) factors or secondary factors related tolifestyle, environment, or medication. Metabolic syndrome is a specific collection of conditions that can increase therisk of diabetes, stroke and heart disease and it affects 25% of the adult world population. In the past few years,
several expert groups have attempted to set forth simple diagnostic criteria to be used in clinical practice to identify
patients with metabolic syndrome. These risk factors
include elevated waist circumference, elevatedtriglycerides, reduced high-density lipoprotein cholesterol,
elevated blood pressure and elevated fasting glucose. The
magnitude of the increased risk can vary according towhich components of the syndrome are present plus theother, non-metabolic syndrome risk factors in a particularperson.

VI

Cardiac & Metabolic Markers for Assay Development

Product Quickfind List
A
Adiponectin..................................... 5
Albumin........................................... 5
Angiotensin..................................... 6
Apolipoprotein A & B...................... 6
Apolipoprotein C............................. 7
Apolipoprotein E.............................. 7
B
BNP, ProBNP & NT-ProBNP............ 8
C
Carboxy Methyl Lysine (CML) ....... 8
sCD40 Ligand (sCD40L)................. 9
Collagen Type I & III........................ 9
C-peptide......................................... 9
C-Reactive Protein (CRP)................ 9Creatine Kinase BB (CK-BB)......... 10
Creatine Kinase MB (CK-MB)....... 10
Creatine Kinase MM (CK-MM)..... 10
Cystatin C...................................... 11D
D-Dimer......................................... 11
E
Epidermal Growth Factor (EGF) ... 11
F
Fatty Acid Binding Protein (FABP).12
Fibrinogen/ Fibrinopeptide A......... 12G
Galectin-3...................................... 12
Ghrelin........................................... 12
Glycogen Phosphorylase
Isoenzyme BB (GPBB).................. 12
H
Hemoglobin A1c (HbA1c)............. 13
IInterleukin 8 (IL-8)......................... 13

L
Lectin-like Oxidized LDL
Receptor-1 (sLOX) Soluble Form....13
Lipoproteins HDL, LDL & VLDL... 13
Lipoprotein-AssociatedPhospholipase A2 (Lp-PLA2)........ 14
Low Density Lipoprotein
Receptor-Related Protein (LRP).... 14
M
Malondialdehyde (MDA) .............. 14
Monocyte Chemotactic Protein 1(MCP-1/MCAF).............................. 14
Myeloperoxidase (MPO)............... 14
Myoglobin (MYO).......................... 15
N
Neuron Specific Enolase (NSE).... 15
Neutrophil Gelatinase-AssociatedLipocalin (NGAL)........................... 15P
Plasminogen Activator
Inhibitor 1 (PAI-1)........................... 16
Pregnancy Associated Plasma
Protein A (PAPP-A)........................ 16
Procalcitonin (PCT)........................ 16
Procollagen Type III....................... 17
Proinsulin....................................... 17

R
Relaxin........................................... 17
Renin............................................. 17
S
S-100 ............................................ 18
S-Adenosyl-Homocysteine (SAH)...18
Serum Amyloid A.......................... 18
Stromal Cell-Derived Factor 1
alpha (SDF-1α)............................... 18T
Thrombomodulin........................... 19
Tissue Plasminogen
Activator (tPA)............................... 19
Troponin Complex ........................ 19
Troponin C-Cardiac (cTnC)............. 19
Troponin I-Cardiac (cTnI)............... 19
Troponin I-Skeletal (sTnI)............... 20
Troponin T-Cardiac (cTnT).............. 20
Tumor Necrosis Factor alpha(TNF-alpha).................................... 21
U
Uroguanylin................................... 21
V
Vitamin D....................................... 21

www.MeridianLifeScience.com

1

Leading Cardiac Markers
Antibodies & Matched Pairs
Brain Natriuretic Peptide (BNP), ProBNP + NT-ProBNP

D-dimer

H86451M	MAb to NT-proBNP a.a. 61-76 (Capture),
ELISA & WB
H86912M	MAb to NT-proBNP a.a. 1-12 (Detection),
ELISA & WB

N01273M
N01274M* Recognizes BNP, proBNP and NT-proBNP.

K4A660R	PAb to BNP (Rabbit) ELISA, RIA,
& IHC, lyophilized
H01420M	MAb to NT-proBNP a.a. 61-76 (Capture),
ELISA & WB
H01419M	MAb to NT-proBNP a.a. 15-20 (Detection),
ELISA & WB
H01423M	MAb to NT-proBNP a.a. 15-20 (Alternate
Detection), ELISA & WB
* Detects NT-proBNP and proBNP.

H01422M	MAb to NT-proBNP a.a. 5-12 (Capture),
ELISA & WB
H01424M	MAb to NT-proBNP a.a. 22-36 (Detection),
ELISA & WB
* Detects NT-proBNP regardless of glycosylation.

C-Reactive Protein (CRP)
M86005M
M01239M

MAb (Capture), ELISA
MAb (Detection), ELISA* Recognizes antigen in the presence and in the absence of Ca2+.

Creatine Kinase (CK)
MAC01-288 MAb to CK-BB, ELISA
H31795M	MAb to CK-MB (Capture/Detection),
ELISA & LF
K31315G	PAb (Goat) to CK-MM (Capture/Detection),
ELISA & LF
* Abs can be used as capture or detection in a sandwich assay.

MAC02-186 MAb to CK-MM, ELISA* 50% cross reactivity with CK-MB and < 1% cross reactivity with CK-BB.

MAC02-512 MAb to CK-MM, ELISA* 52% cross reactivity with CK-MB, and no cross reactivity with CK-BB.

H86023M
H86218M
H86013M

N01270M	MAb (Capture), ELISA, LF & WB
N01269H MAb (Detection), ELISA, LF & WB

* Highly specific for D-dimer and other cross linked fibrin degradation products
containing D-dimer, but no reactivity with Fragment D or E. No cross-reaction
was found with purified intact fibrinogen or fibrinogen degradation products.

N86304M	MAb (Capture), ELISA & WB
N86352M 	MAb (Detection), ELISA & WB

* Reacts with high molecular weight fibrin degradation products. Does notcross react with fibrinogen or D-monomer.

Fatty Acid Binding Protein (FABP)
H01324M
H01323M

MAb (Capture), ELISA & LF
MAb (Detection), ELISA & LF

Fibrinogen
N86413M

MAb, ELISA & WB

Galectin-3
K01381M

MAb, ELISA* Reacts with human and mouse Galectin-3.

Glycogen Phosphorylase Isoenzyme BB (GPBB)
H86807M

MAb, ELISA & WB

Lipoprotein-Associated Phospholipase A2 (Lp-PLA2)
H01390M
H01393M
H01389M

MAb (Capture), ELISA
MAb (Detection), ELISA
MAb (Alternate Detection), ELISA

H01392M
H01393M

MAb (Capture), ELISA
MAb (Detection), ELISA

Myeloperoxidase (MPO)
H87207M

Cystatin C

MAb (Capture), ELISA & WB
MAb (Detection), ELISA & WB

MAb, ELISA & IHC* Recognizes both native and recombinant human MPO.

MAb (Capture), ELISA
MAb (Detection), ELISA
MAb (Alternate Detection)

2 2| Cardiac
Cardiac& &Metabolic
MetabolicMarkers
MarkersforforAssay
AssayDevelopment
Development

Myoglobin (MYO)
H01328M
H01327M

MAb (Capture), ELISA & LF
MAb (Detection), ELISA & LF

Procalcitonin (PCT)
E86561M
E86420M

MAb (Capture), ELISA & WB
MAb (Detection), ELISA & WB

E86412M
E86813M

MAb (Capture), ELISA
MAb (Detection), ELISA* Specific to procalcitonin and calcitonin.

* Specific for procalcitonin and katacalcin.

Pregnancy Associated Plasma Protein A (PAPP-A)

Troponin T-Cardiac (cTnT)

E86141M
E86910M

MAb (Capture), ELISA & WB
MAb (Detection), ELISA & WB

H86429M	MAb to a.a. 60-70 (Capture), ELISA & WB
H86111M	MAb to a.a. 95-181 (Detection), ELISA & WB

* Detects both heterotetrameric (found in placental blood) and homodimeric(found in atherosclerotic plaques) PAPP-A.

* Does not cross react with sTn.

E86114M

MAb, ELISA & WB

Vitamin D

S-Adenosyl-Homocysteine (SAH)
K01395C

PAb (Chicken), ELISA* Affinity purified, treated with Adenine-9--D-Ribofuranoside to remove crossreaction to Adenosine.

Soluble CD40 Ligand (sCD40L)
P86106M
P42374M

MAb, ELISA
MAb, FC & IFA

K01214M MAb to Vitamin D (25 OH), ELISA, LF,
CLIA & ELISA* Pairs with antigens A01697B & A01698B.

K01213M

MAb to Vitamin D (25 OH), ELISA & RIA* Pairs with antigens A01697B & A01698B.

K24124M-LQ MAb to 25-OH Vitamin D3, ELISA
K24124M MAb to 25-OH Vitamin D3, ELISA
K24123M MAb to 1,25 (OH)2 Vitamin D3, ELISA*Recognizes Native Human 25 OH Vitamin D2 and 25 OH Vitamin D3.

Troponin I-Cardiac (cTnI)
H86285M	MAb to a.a. 86-90 (Capture), ELISA & WB
H01347M	MAb to a.a. 24-40 (Detection), ELISA & WB
* Reacts equally with free cTnI and complexed cTnI, does not cross react with sTn.

H01340M
H86241M
H86596M

MAb to a.a. 41-49, ELISA
MAb to a.a. 87-91, ELISA & WB
MAb to a.a. 169-178, ELISA & WB

K31015M	MAb (Capture), ELISA
K31341G	PAb to a.a. 27-39 (Goat, Detection), ELISA,
WB & IHC
K31342G	PAb to a.a. 69-86 (Goat, Alternate
Detection), ELISA & IHC* Cross reactivity with sTn is < 0.1% (ELISA).

www.MeridianLifeScience.com
www.MeridianLifeScience.com |3 3

Leading Cardiac Markers
Human Antigens (for use as standards, controls & calibrators)
Brain Natriuretic Peptide (BNP), ProBNP + NT-ProBNP

Lp-PLA2

A24760H	ProBNP, recombinant protein (E. coli) of
N-terminal (a.a. 1-76, MW 8.39 kDa)

A01413H Recombinant (E.coli),> 95% pure (SDS-PAGE)

A01261H	NT-proBNP, recombinant protein (E. coli) of
N-terminal (MW 8.589 kDa). Contains anadditional methionine residue at the
N-terminus (in comparison with native
NT-proBNP)

Myeloperoxidase (MPO)

N86583H	BNP and NT-proBNP Free Plasma, fromnormal human plasma, used for preparation of
any proBNP-related standards and calibrators

Myoglobin

A97201H	From human fluids and reactive withmonospecific goat anti-CRP, 95% pure
(SDS-PAGE)

Pregnancy Associated Plasma Protein A (PAPP-A)

Creatine Kinase (CK)VTI830	CK-BB, produced in cell culture (yeast),
represents native full length protein and isenzymatically active
VTI840	CK-MB Isoenzyme Type I, produced in cell
culture (yeast), enzymatically active

A38104H	From human neutrophils, > 96% pure
(SDS-PAGE), approximate activity of 1,100
Units/mg Protein

A38131H From human heart, 99% pure (SDS-PAGE)

A86864H	From human retroplacental blood.
Heterotetrameric complex consisting of
PAPP-A and pro-MBP subunits, > 85% pure
(SDS-PAGE), lyophilized
Procalcitonin (PCT)
A01367H	Recombinant protein (E. coli) representing a.a.
3-116 which corresponds to the unprocessed
human protein, contains a 6-His tag at N-terminal

VTI810	CK-MB Isoenzyme Type II, produced in cell
culture (yeast), represents native full length
protein and is enzymatically active

Troponin (cTnT & cTnI)

VTI850	CK-MM Isoenzyme Type I, produced in cell
culture (yeast), represents full length protein
without the C-terminal lysine on bothsubunits and is enzymatically active

A38150H	cTnI, from human heart, > 98% pure
(SDS-PAGE), single band ~24 kDa

VTI820	CK-MM Isoenzyme Type III, produced in cell
culture (yeast), represents full length protein
and is enzymatically active
D-Dimer
A01408H From human plasma, > 90% pureA01463H

From human plasma, > 95% pure (SDS-PAGE)

Fatty Acid Binding Protein (FABP)
A86865H From human heart, > 95% pure (SDS-PAGE)

4 4| Cardiac
Cardiac& &Metabolic
MetabolicMarkers
MarkersforforAssay
AssayDevelopment
Development

A86813H	cTnT, from human heart, > 98% pure
(SDS-PAGE)

Vitamin D
A01694D	
25-OH Vitamin D3 BSA Conjugate, ≥ 99%
Pure (HPLC)
A01698B	Vitamin D Biotin Conjugate, > 95.7%
Pure (HPLC)
A01697B Vitamin D BSA Conjugate
A50674H	
Vitamin D Binding Protein (Gc-Globulin),> 95% Pure (SDS-PAGE)
A01409H

1,25 Dihydroxy Vitamin D3, > 99% Pure (HPLC)

Product list
Abbreviations
6-His – Polyhistidine-tag
Aff.Pur. – Affinity Purified, analyte-specific column
Alk.Phos. – Alkaline Phosphatase conjugated productC. Eggs – Chicken Eggs
CLIA – Chemiluminescence Immunoassay
CVD – Cardiovascular Disease
DB – Dot Blot
DFA – Direct Immunofluorescence Assay

Adiponectin
A protein hormone that modulates a number of metabolic
processes including glucose regulation and fatty acid oxidation.
Low levels of adiponectin are associated with the increased
prevalence of obesity-linked cardiovascular disease, including
ischemic heart disease and peripheral arterial disease.

Antibody Pairs• Suitable for use in ELISA• MAbs produced in vivo
CAPTURE
DETECTION

GST – Glutathione S-transferase

H01263M
H01286M
H01296M
H01297M

HRP – Horseradish peroxidase conjugated productAntigens

IB – Immunoblot

A01260H		

FC – Flow Cytometry
FITC – Fluorescein conjugated product

ICC – ImmunocytochemistryH01262M
H01285M
H01297M
H01296M* Detects total Adiponectin* Detects total Adiponectin* Detects total Adiponectin* Detects total Adiponectin• Native antigen from pooled human plasma• Affinity purified, > 95% pure (SDS-PAGE)IEP – Immunoelectrophoresis
IFA – Immunofluorescence Assay
IgG – Immunoglobin G
IgM – Immunoglobin M
IHC – Immunohistochemistry
IP – Immunoprecipitation
LF – Lateral Flow
Lysate – Cells which have been lysed
MAb – Monoclonal antibody
Monospecific – Single band when tested byimmunoelectrophoresis
Neat – Whole, unpurified, undiluted antisera
Neph – Product has been quality controlled byNephelometry
PAb – Polyclonal antibody
Purified/IgG – Refer to the Certificate of Analysis
regarding the extent of purification and the purification
process used.
RIA – Radioimmunoassay

AlbuminHypoalbuminemia is a medical condition in which blood levelsof albumin are abnormally low. It is a common condition inpatients with heart failure and becomes more prevalent withincreasing age and illness. It can be used as an independent
predictor of incident heart failure in end-stage renal disease
and elderly patients.

Monoclonals• Recognizes human serum albumin in ELISA• Produced in vivo
H86424M

Antibody Pairs•	Suitable for use in ELISA, WB and the quantitative
detection of human serum albumin in serum and urine•	Does not react with bovine serum albumin or egg white albumin• MAbs produced in vivo
CAPTURE
DETECTION

RID – Radial Immunodiffusion

H86157M
H86157M

SDS-PAGE – Sodium Dodecyl Sulfate Polyacrylamide
Gel Electrophoresis

Antigens

TIA – Product has been quality controlled by
Turbidimetry

H8P01-767
		

H86910M
H86611M• Native antigen from aphoresis plasma• Low B12 and low folate

WB – Western blot

www.MeridianLifeScience.com
www.MeridianLifeScience.com |5 5

Product list | Continued
Angiotensin

Apolipoprotein A-II (Apo A-II)

Angiotensin is a peptide hormone that causes vasoconstriction
leading to high blood pressure. It is part of the renin-angiotensin
system, which is a major target for drugs that lower bloodpressure. Angiotensin I is converted to Angiotensin II throughthe enzyme angiotensin-converting enzyme (ACE). ACE inhibitors
are widely used in the treatment of arterial hypertension andcardiovascular diseases.

Apo A-II is the second most abundant protein of HDL particles
and plays an important role in reverse cholesterol transportand lipid metabolism. Apo A-II is mainly produced in the liver andmodulates lipoprotein lipase and hepatic triglyceride lipase.
The Apo A-II test can be used as an aid in assessing the riskof CVD.

Monoclonals

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

Apolipoprotein A-I (Apo A-I)

K45890G
K97211R
G5C21-766
K34001G
K34004G
K74001P

Goat
Rabbit
Goat
Goat
Goat
Goat

Neat
Aff.Pur.
Aff.Pur.
Aff.Pur.
BiotinHRP

EIA,WB
EIA,IBIEP
EIA,WB
EIA,WB
EIA,IBApo A-I is the major protein component of high density
lipoprotein (HDL) in plasma and plays a role in lipid metabolism.

Apolipoprotein B (Apo B)• Suitable for use in ELISA• Produced in vivo
E01296M (Angiotensin I) * Does not cross-react with II and III
E01297M (Angiotensin II) * Cross-reacts with I and III

Monoclonals• Does not cross-react with Apo A-II or Apo B• Suitable for use in RIA and ELISA• Produced in vivo
MAC20-001
MAC20-029
H45402M
H45404M
H45625M

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

K45252G
K45252P
K01234G
K23600G
K04329G

Goat
Goat
Goat
Goat
Goat

Aff.Pur.
HRP
Monospecific
Neat
Monospecific

EIA,WB
EIA,WB
IEP
RID
IEP* K97110S is specific for ApoA only (not ApoA I)

6 6| Cardiac
Cardiac& &Metabolic
MetabolicMarkers
MarkersforforAssay
AssayDevelopment
Development

Apo B is the primary apolipoprotein in LDL particles and highlevels of Apo B and occurs in the plasma in two main isoforms,
Apo B48 and Apo B100. Apo B48 is a unique protein tochylomicrons from the small intestine and Apo B100 is foundin lipoproteins originating from the liver. It is well establishedthat Apo B100 levels are associated with coronary heartdisease, and serve as a better predictor of LDL levels.

Monoclonals• Suitable for use in ELISA and RIA• Produced in vivo
MAC23-031
MAC23-035
H45160M
H45161M
H45640M

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

Apo B
G5C23-766
K01235G
K04323G
K62200G

Goat
Goat
Goat
Goat

Aff.Pur.
MonospecificMonospecificMonospecific

N/A
IEP
IEP
EIA,IEP,TIA

Apolipoprotein B (Apo B) continued

Apolipoprotein CIIICATALOGA protein that regulates both lipoprotein lipase and lecithincholesterol acyltransferase and may cause hypertriglyceridemia.
Plasma levels of lipoprotein Apo CIII predict coronary heartdisease and are associated with metabolic syndrome.

SOURCE

Apo B–48/100
K45253G
Goat
K34003G
Goat
K34005G
Goat

FORMATAPPLICATION

Aff.Pur.
BiotinHRP

EIA,WB
EIA,WB
EIA,WB

Antigens
A34300H		• Native Apo B100 from human plasma LDL• Cross-reacts with anti-LDL

Apolipoprotein AI/B (Apo AI/B) Calibrator
Antigens
A62934H
				• Native antigen from human plasma• Apo AI: 313.0 mg/dL (lot dependent)• Apo B: 307.0 mg/dL (lot dependent)

Apolipoprotein CII (Apo CII)
Apo CII acts as a co-factor for lipoprotein lipase, an enzymethat hydrolyses triglycerides in chylomicrons and VLDL. Can beused as an aid in assessing the risk of cardiovascular disease.

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

K74322G
K97112R

Goat
RabbitNeat
Aff.Pur.

EIA,IB
EIA,IB

Antigens
A50302H		• Native antigen from human plasma VLDL
• 95% pure (SDS-PAGE)

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

K74130G
K74140G
K74140P
K74170B
K97113R

Goat
Goat
Goat
Goat
RabbitNeat
Aff.Pur.
HRP
BiotinAff.Pur.

EIA,IB
EIA,IB
EIA,IB
EIA,IB
EIA,IB

Antigens
A34129H
		• Native antigen from human plasma (VLDL)• 95% pure (SDS PAGE)

Apolipoprotein E (Apo E)
A major component of very low-density lipoproteins (VLDLs)
and binds to a specific receptor on liver and peripheral cells.
There are 3 major alleles: E3, E4, and E2. E3 is the normal form
with normal funciton, the E2 variant has a reduced ability bind
receptors and is associated with a high risk of CVD and the E4variant is associated with increased levels of LDL- cholesteroland decreased levels of HDL-cholesterol, which is associatedwith a higher risk of developing coronary artery disease.

Monoclonals•	Reacts with VLDL of human plasma• Suitable for use in ELISA and IHC• Produced in vivo, lyophilized
H61529M

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

G5C27-766
K34002G
K74190G
K74180B

Goat
Goat
Goat
Goat

Aff.Pur.
HRP
Aff.Pur.
BiotinN/A
EIA,WB
EIA,WB
EIA,WB

Antigens
A50120H		• Native antigen from human plasma (VLDL)• > 95% pure (SDS-PAGE)

www.MeridianLifeScience.com
www.MeridianLifeScience.com |7 7

Product list | Continued
Antigens

BNP, ProBNP & NT-ProBNP

R01614				• proBNP recombinant (E. coli)• Suitable for enzyme linked fluorescent assay• 95% pure (Silver Staining SDS-PAGE)

A24108H
		• proBNP recombinant (E. coli)• > 95% pure (SDS-PAGE)

A01455H				• proBNP recombinant (E. coli)• Suitable for use in ELISA as a calibrator,
standard or an immunogen for antibody
development• Contains one additional Methionine residue at
the N-Terminus (in comparison with native
proBNP)• > 95% pure (Tricine SDS-PAGE)

Antibody Pairs

A24810H				• proBNP, C-terminal (synthetic)• Represents amino acids 1-32
• > 95% pure (HPLC), lyophilized• Suitable for use in ELISA and WB• Specific for Human BNP unless otherwise specified
CAPTURE
DETECTION

R01616				• NT-proBNP recombinant (E. coli)• > 95% pure (Silver Staining SDS-PAGE)• Suitable for enzyme linked fluorescent assay

H86051M
H86051M
H01365M
H01365M
H01365M
H86051M
H86451M
H86451M
H86451M
H86511M
H01420M
H01420M
H01420M

A01261H				• NT-proBNP recombinant (E. coli)• Suitable for use in ELISA• > 95% pure (Tricine SDS-PAGE)

A24607H				• proBNP, N-terminal recombinant (synthetic)• Represents a.a.1-76• > 95% pure (HPLC), lyophilized

A24760H				

• proBNP, N-terminal recombinant (E. coli)• Represents the N-terminal a.a.1-76• > 95% pure (SDS-PAGE), lyophilized

BNP (B type natriuretic peptide) is a hormone and importantbiomarker with an established role in the diagnosis of congestive
heart failure. Its utility has also been explored in myocardialischemia and infarction, and in acute pulmonary embolism. When
BNP is secreted it is attached to a 76–amino acid N-terminal
fragment called NT-proBNP which is biologically inactive. Oncereleased, BNP binds to and activates the atrial natriuretic factor(ANP) receptors.

Monoclonals• Reacts with proBNP, N-terminal•	Suitable for proBNP and NT-proBNP immunoassay
H86705M
a.a. 13-27
H86010M
a.a. 28-45*Also works in WB
H86916M
a.a. 46-60*Also works in WB
H86288M
a.a. 61-76

H86262M
H01365M
H86507M
H86573M
H01366M
H86915M
H86132M
H86912M
H86915M
H86214M
H01422M
H01419M
H01423M*Recognizes BNP & pro-BNP*Recognizes pro-BNP*Recognizes proBNP & NT-proBNP*Recognizes proBNP & NT-proBNP*Recognizes proBNP & NT-proBNP*Recognizes proBNP & NT-proBNP		*Detects NT-proBNP and proBNP*Detects NT-proBNP and proBNP*Detects NT-proBNP and proBNP

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

K4A660R
K4A670R
K24410R

RabbitRabbitRabbitNeat
Purified
Neat

EIA,IFA,IHC,RIA
EIA,IHC,RIA
RIA

8 8| Cardiac
Cardiac& &Metabolic
MetabolicMarkers
MarkersforforAssay
AssayDevelopment
Development

Carboxy Methyl Lysine (CML)
A well-characterized glycoxidation product, formed from theoxidation of both carbohydrates and lipids, which accumulates
in tissues with age. CML is a biomarker of general oxidative
stress which is involved in the pathogenesis of atherosclerosis.

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

K97135G

Goat

Aff. Pur.

EIA,WBMonoclonals

sCD40 Ligand (sCD40L)
A member of the TNF superfamily primarily expressed on
activated T cells. Studies suggest that sCD40L may be at theheart of the atherosclerotic process, specifically playing a rolein the inflammatory aspects of atherosclerotic lesion progression,
thrombosis and restenosis.

Monoclonals• Suitable for use in ELISA and WB•	Can be used for detection of human sCD40L in serum
P86210M• Reacts with human free C-peptide and Proinsulin• Suitable for use in ELISA and RIA• Produced in vivo
E86588M• Reacts with C-peptide• Suitable for use in ELISA and RIA
E86710M
MHP18-601
MHP18-6014 * Biotin conjugated

Collagen Type I & III

C-Reactive Protein (CRP)

Increased myocardial collagen accumulation is present inalmost every cardiac disease and plays an important rolein the reduced heart function. N-terminal and C-terminal
propeptides of Collagen Type I and III are the two majorcollagen types in the heart and reflect collagen synthesis anddegradation. Their role as serum biomarkers for the diagnosisof cardiac fibrosis is being studied.

CRP is mainly used as a maker of inflammation. Highsensitivity CRP assays can be used for determining the riskof cardiovascular disease, heart attacks, and stroke.

Monoclonals• S pecific for both native and heat denatured human
Collagen Type I• Suitable for ELISA and IHC
M1A340M

Polyclonals
CATALOG

SOURCE

Collagen Type III
T40233R
RabbitFORMATAPPLICATION

Purified

EIA,IFA,IHC

Antigens
A33123H
				• Native Human Collagen Type III• Sourced from placental villi, > 90% pure
(SDS-PAGE)• Suitable as a immunoassay standard and for
antibody production

C-peptide & Proinsulin
C-peptide is a combination of amino acids that are a by-productof insulin and the level of this protein indicates how much insulin
the pancreas is producing. Studies have shown an association
between serum C-peptide levels and risk of cardiovascular-relateddisease and coronary artery-related mortality among adults
without diabetes.

Antibody Pairs• Suitable for use in ELISA, high sensitivity• MAbs produced in vivo
CAPTURE
DETECTION
M86005M
M01243M
M01331M

M01239M
M01239M
M01330M* Recognizes CRP with or without Ca2+.
* Recognizes CRP with or without Ca2+.

Monoclonals• Recognizes CRP with or without the presence of Ca2+
• Suitable for use in ELISA• Produced in vivo
M01238M* Can also be used in IHC and WB
M66112M
M66113M
M86007M* Can also be used in IHC
M86842B* Biotin conjugatedM86842M• Recognizes CRP• Suitable for use in ELISA (Unless otherwise specified)• Produced in vivo
M01319B* Biotin conjugatedM01319M
M01295M* No applications have been tested

www.MeridianLifeScience.com
www.MeridianLifeScience.com |9 9

Product list | Continued
C-Reactive Protein (CRP) continued

Creatine Kinase MB (CK-MB)

Polyclonals

CK-MB is expressed in heart muscle and it is used in the diagnosisof acute myocardial infarction and possible reinfarction. The halflife of CK-MB in the circulation is relatively short (approximately
12 hours). CK-MB is relatively cardiac-specific, even healthy people
may have low concentrations of this isoenzyme in their blood.

CATALOG

SOURCEFORMATAPPLICATION

L01237G
M01333C

Goat
C. EggPurified
Aff. Pur.

IEP
N/A

Antigens

Antibody Pairs

A01430H		• Native antigen from human fluids• 98% pure (SDS-PAGE)

A01250H
		• Recombinant (E. coli)• Pentamer structure• Suitable for use in ELISA and LF• MAbs produced in vivo
CAPTURE
DETECTION
H31795M

K31315G* K31315G is a polyclonal Goat
anti-CK-MM

K31315G

H31795M* K31315G is a polyclonal Goat
anti-CK-MM

H01330M

H01329M

Antigens

Creatine Kinase BB (CK-BB)
CK-BB is found mostly in the brain and increases in response
to brain injury, meningitis, abnormal cell growth, severe shock,stroke, hypothermia, or restricted blood flow to the bowel. It is
used in creatine kinase test which determines what percentageof the total creatine kinase is due to the type produced bydamaged heart muscles (CK-MB).

Monoclonals• Produced in vivo
MAC01-288

Antigens
VTI830						• Recombinant (P. pastoris)• Full length protein with amino acid sequence
identical to the native enzyme• > 95% pure (SDS-PAGE)• Suitable for use in ELISA

H6C01-323
		• Native antigen from human brain• Activity: 730 Units/mL at 37ºC (lot dependent)

VTI840		• Recombinant (P. pastoris), Isoenzyme Type I• Suitable for use in ELISAVTI810		• Recombinant (P. pastoris), Isoenzyme Type I• Suitable for use in ELISA

Creatine Kinase MM (CK-MM)
Found in your skeletal muscle and heart. CK-MM generally rises
if you have muscle damage in your heart, brain, or skeleton
after a crush injury, seizures, muscular dystrophy, muscle
inflammation, or another skeletal muscle disorder. It is used increatine kinase test which determines what percentage of thetotal creatine kinase is due to the type produced by damaged
heart muscles (CK-MB).

Monoclonals• Suitable for use in ELISA• Produced in vivo
• 52% cross-reactivity to CK-MB• 0% Cross-reactivity to CK-BB
MAC02-186
MAC02-512

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

G5C02-766
H01310G
K31315G

Goat
Goat
Goat

Aff.Pur.
Purified
Aff.Pur.

N/A
N/A
EIA,LF* K31315G pairs with MAb to CK-MB H31795M

1010| Cardiac
Cardiac && Metabolic
Metabolic Markers
Markers forfor Assay
Assay Development
Development

Creatine Kinase MM (CK-MM) continued
Antigens
VTI850				• Recombinant (P. pastoris), Isoenzyme Type I• Full length protein without the C-terminal
lysine on both subunits• Suitable for use in WB and ELISAVTI820				• Recombinant (P. pastoris), Isoenzyme Type I• Identical amino acid sequence to native protein• Suitable for use in ELISA

A38925H• Native antigen from human heart, lyophilized

Cystatin C
Traditionally used as a biomarker of kidney function, it has
recently been studied for its role in predicting new onset or
deteriorating CVD. In different cardiovascular conditions, Cystatin
C is a sensitive marker for renal dysfunction, a predictor of thedevelopment of heart failure, and is independently associatedwith subsequent CVD and outcome.

Antibody Pairs• Suitable for use in ELISA• MAbs produced in vivo
CAPTURE
DETECTION
H86023M

H86013M

Antigens
A01419H• Native antigen from human urine

D-DimerD-dimer is a marker of fibrin turnover and elevated levels areindicative of the presence of a clot and have been reported indeep vein thrombosis, pulmonary embolism, disseminatedintravascular coagulation, acute aortic dissection, myocardial
infarction, malignant diseases, obstetrical complications, third
trimester of pregnancy, and surgery. It is a marker for the early
diagnosis of acute coronary syndromes and can be used inaddition to traditional assessment for myocardial infarction.

Antibody Pairs• Suitable for use in ELISA and WB• Reactive with D-Dimer and other fribin degradation products• MAbs produced in vivo
CAPTURE
DETECTION
N01270M

N01269H* Does not cross-react Fragment D or
E, also suitable for LF

N01273M
N86304M
N86304M

N01274M
N86925M
N86043M* Does not cross-react with Fibrinogen

Antigens
A01408H
		• Native antigen from human plasma• > 90% pure (SDS-PAGE and gel-scanning)

A01463H
				• Native antigen from human plasma• Suitable for ELFA
• 95% pure (SDS-PAGE)

A86870H		• Native antigen from human plasma• > 90% pure (SDS-PAGE and gel-scanning),lyophilized

Epidermal Growth Factor (EGF)
EGF is a growth factor with potential use as cardiac biomarker
due to its role in the proliferation of cardiac stem cells. Researchsuggests that the human heart has an endogenous reserve of
cardiac stem cells (CSCs) which can be activated by various
growth factors to reconstitute injured myocardium. EGF has
shown to significantly promote the proliferation, migration, andwound healing activities of these CSCs in comparison to theother growth factors.

Antigens
VTI880				• Recombinant (P. pastoris)• Suitable for use in WB• >95% pure (SDS-PAGE)

Monoclonals•	Does not cross-react with fibrinogen and D-monomer(unless noted otherwise)•	
Suitable for use in ELISA and WB
N86925M* Reacts with high molecular weight fibrin degradation products
N86925B* Biotin conjugatedwww.MeridianLifeScience.com
www.MeridianLifeScience.com |1111

Product list | Continued
Fatty Acid Binding Protein (FABP)

Galectin-3

Heart type FABP is a small cytoplasmic protein (15 kDa) released
from cardiac myocytes following an ischemic episode. It is anearly biomarker for myocardial infarction, detected in the blood
within one to three hours of acute coronary syndrome.

Galectin-3 is a galactoside-binding protein G that binds to andactivates myofibroblasts in damaged heart tissue, leading tocollagen synthesis and subsequently problematic fibrosis. It has
been correlated with the prognosis in patients with coronary
heart failure and the first Galectin-3 biomarker assay wasapproved by the FDA in 2010.

Antibody Pairs•	Suitable for use in ELISA• MAbs produced in vivo
CAPTURE
DETECTION
H86101M
H01314M
H01314M
H01324M

H86505M
H86101M
H01315M
H01323M

Monoclonals
K01381M
K01382M* Also works in LF assays

Antigens
A86865H
				• Native antigen from human heart• Suitable for use as a immunoassay standard,
immunogen for antisera production, or a tracer
for iodination• > 95% pure (10-20% gradient SDS-PAGE)

R01617
				• FABP3 Recombinant (E.coli)• Suitable for use in ELISA• 95% pure (silver staining SDS-PAGE)*Suitable for use in FC, produced in vivo
*Suitable for use in FC, produced in cell culture

GhrelinClinical studies suggest a role for ghrelin in the treatment of
CHF. Known cardiovascular effects of ghrelin include loweringof peripheral resistance either direct at the vascular level, and/or by modulating sympathetic nervous activity. Gherlin also has
potential as a marker of abnormal metabolism in cardiac failure.

Monoclonals• Suitable for use in ELISA• Produced in vivo
E86621M

Fibrinogen/ Fibrinopeptide A

Glycogen Phosphorylase Isoenzyme BB (GPBB)

Fibrinogen is a plasma glycoprotein that is essential for blood
blotting and has the ability to promote thromboses, or clots, bycausing platelets to clump inside blood vessels. A number ofstudies have shown elevated fibrinogen levels to be a major risk
factor for coronary heart disease.

GPBB is an isoenzyme of glycogen phosphorylase and a key
enzyme of glycogenolysis. It has been suggested as an earlymarker of acute myocardial infarction and unstable angina.

Antibody Pairs• Suitable for use in ELISA•	For specific fibrinogen detection, pair a capture
anti-fibrinopeptide A antibody with a detection
anti-fibrinogen antibody• MAbs produced in vivo
CAPTURE
DETECTION
N86710M

N86413M* N86710M is specific for
Fibrinopeptide A

1212| Cardiac
Cardiac&&Metabolic
MetabolicMarkers
Markersfor
forAssay
AssayDevelopment
Development

Monoclonals• Suitable for use in ELISA and WB• Produced in vivo
H86807M

Hemoglobin A1c (HbA1c)

Lipoproteins HDL, LDL, VLDL Receptor

As glucose circulates in the blood, some of it spontaneously
binds to hemoglobin to form hemoglobin A1c (HbA1c). The
development of specific diabetes complications correlates withglycated haemoglobin (HbA1c), the most accepted measure ofchronic glycaemia.

Lipoprotein diagnostics, such as LDL cholesterol and HDLcholesterol, help to diagnose dyslipidemia which is an importantrisk factor for cardiovascular disease and type II diabetes.

Monoclonals• Suitable for use in sandwich ELISA• Does not cross-react with HbA10• Produced in vivo
H01331M* Can also work in indirect ELISA (antigen capture)
H01388M* Can also work in WB

Antigens
A01273H
		• Native antigen from human erythrocytes• > 96% HbAlc (HPLC analysis using a
Mono S Column)

Interleukin 8 (IL-8)
IL-8 is a neutrophil chemokine produced by macrophages,
lymphocytes, epithelial cells and endothelial cells, and promotes
neutrophil chemotaxis. Studies in patients with CAD havedemonstrated that IL-8 may predict cardiovascular events
independent of the other cytokines and high sensitivity CRP.

Antigens
A42280H
		
A42208H
		• Recombinant (E. coli), a.a. 1-72
• > 98% pure (HPLC, FPLC and reducing andnon-reducing SDS-PAGE)• Recombinant (E. coli), a.a. 1-77
• > 98% pure (HPLP and SDS-PAGE)

Monoclonals• MAb to LDL Receptor-Related Protein (LRP)• Will not block ligand binding• Suitable for use in ELISA, WB, IHC, and FC
H63650M

Antigens
A95132H
				• Native HDL from human normolipidemic plasma• Devoid of VLDL, IDL and LDL• Ultracentrifugation at density 1.063–1.210

A95322H• Native HDL 2 (HDL2) Subfraction from humannormolipidemic plasma• Devoid of VLDL, IDL, LDL, and HDL3
• Ultracentrifugation density at 1.063 - 1.125

				
A95332H
				• Native HDL 3 (HDL3) Subfraction from humannormolipidemic plasma• Devoid of VLDL, IDL, LDL, and HDL2
• Ultracentrifugation density at 1.120–1.21g/mL

A34275H
		• Reconstituted HDL from human plasma• A complex of human Apolipoprotein A-I and
1-palmitoyl-2-oleoyl phosphatidylcholine
prepared at a molar ratio of 1 to 100 by thecholate removal method

A34013H
				• Native VLDL from human plasma• Devoid of IDL, LDL, and HDL• Purification by ultracentrifugation atdensity 1.006

Lectin-like Oxidized LDL Receptor-1(sLOX) Soluble Form
A major endothelial receptor for oxidized low-density lipoprotein
which is believed to play a role in atherosclerosis. Its expression
is induced in pro-atherogenic settings including hypertension,
hyperlipidemia, and diabetes, and it accumulates in therosclerotic
lesions where it has been strongly linked to plaque rupture.
Specifically sLOX-1 levels are elevated in acute coronary
syndrome at an early stage, suggesting its usefulness as an
early diagnostic marker of acute coronary syndrome.

Monoclonals• Specific for human recombinant sLOX-1 (a.a. 58-273)• Suitable for use in ELISA and WB• Produced in vivo
H86922M

www.MeridianLifeScience.com
www.MeridianLifeScience.com |1313

Product list | Continued
Lipoprotein-Associated Phospholipase A2(Lp-PLA2)
Lp-PLA2 is a protein produced by inflammatory cells thatcirculates mainly with LDL and is responsible for hydrolyzing
oxidized phospholipids in LDL. It is highly upregulated inatherosclerotic plaques and is thought to be directly involvedin the development of atherosclerosis and plaque rupture.

Monoclonals

Malondialdehyde (MDA)
MDA is a reactive aldehyde produced by the degradation ofpolyunsaturated lipids and causes oxidative modification of
LDL to form malondialdehyde low density lipoprotein (MDALDL). MDL-LDL is a sensitive biomarker for ACS patients withunstable angina and acute myocardial infarction. MDA-LDL not
only serves as an oxidative stress marker but as a marker ofplaque destabilization.

Polyclonals• Suitable for use in ELISA• Produced in cell culture
H01389M

CATALOG

SOURCE FORMATAPPLICATION

K97120P

Goat

EIA,WB

HRP, Lyophilized

Antibody Pairs• Suitable for use in ELISA• Produced in cell culture
CAPTURE
DETECTION
H01390M
H01390M
H01392M

Monocyte Chemotactic Protein 1(MCP-1/MCAF)

H01393M
H01389M
H01393M

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

H01333S

Sheep

Aff.Pur.

EIA,IHC,WB

Antigens
A01413H
				• Recombinant (E. coli)• > 95% pure (SDS-PAGE)• Contains a His-tag

Low Density Lipoprotein Receptor-Related
Protein (LRP)
LRP is a large multifunctional receptor mediating the clearanceof diverse ligands, including selected lipoproteins, various
protease inhibitor complexes, and thrombospondin. The rangeof ligands recognized by LRP suggests that it plays a role indiverse processes including lipid metabolism, cell growth,migration, and tissue invasion.

Monoclonals• Suitable for use in WB, IHC, and FC• Produced in vivo
H63180M* Specifically reacts with the 515 kDa alpha-chain of human
LRP/alpha2

H63080M* Specific for human light chain of LRP, 85 kDa

1414| Cardiac
Cardiac&&Metabolic
MetabolicMarkers
Markersfor
forAssay
AssayDevelopment
Development

MCP-1 is a cytokine produced by macrophages, smooth muscle
cells and endothelial cells within atherosclerotic plaques and an
important indicator of atherosclerotic plaque burden.
Specifically, high levels of MCP-1 have been associated witha poor prognosis and increased risk for death independent ofother risk factors in patients with ACS.

Antigens
A01251H
				• Recombinant (E. coli)• Non-glycosylated polypeptide chain
containing 76 a.a.
• > 98% pure (RP-HPLC and SDS-PAGE),
lyophilized

Myeloperoxidase (MPO)
MPO is a peroxidase enzyme and a marker of inflammation andneutrophil activation during strenuous exercise. In addition, it has
been implicated in the oxidation of lipids contained in low-density
lipoproteins, infiltration of macrophages and neutrophils,formation of unstable coronary plaques, and plaque rupture.
MPO is elevated in patients with coronary artery disease and canserve as a prognostic marker to identify patients with unstableplaques before complete microvascular obstruction.

Monoclonals• Suitable for use in ELISA• Produced in vivo (unless stated otherwise)
H87207M*Also works in WB, IHC, FC and produced in cell culture
K86005M*Also works in WB

Myeloperoxidase (MPO) continued

Neuron Specific Enolase (NSE)

Antigens

NSE is one of three recognized forms of enolase, an enzyme in
the glycolysis pathway. Studies have shown NSE concentrations
rise in the cerebrospinal fluid following an ischemic stroke.

A01462H
		• Native antigen from human neutrophils• Suitable for use in ELISA

A38104H
		• Native antigen from human neutrophils• > 96% pure (SDS-PAGE)

A50181H		• Native antigen from human neutrophils• 95% pure (SDS-PAGE), lyophilized

Myoglobin (MYO)
Myoglobin is the primary oxygen-carrying pigment of muscle
tissue and it is present in high levels in serum when muscle
tissue is damaged, such as after a heart attack. Myoglobinachieves its maximal diagnostic sensitivity within 5 hours ofsymptom onset and is an early marker of acute myocardial
infarction. However, in patients with concurrent trauma or
renal failure it exhibits poor specificity.

Antibody Pairs•	Suitable for use in ELISA• MAbs produced in vivo (unless noted otherwise)
CAPTURE
DETECTION
H86142M
H86703M

H86703M
H86142M

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

H01370C

C. Eggs

Aff.Pur.

N/A

Antigens
A38131H		• Native antigen from human heart tissue• 99% pure (SDS-PAGE, reduced)

A86850H				• Native antigen from human heart tissue• > 95% pure (SDS-PAGE), lyohphilized• Suitable for use as a reference or standard in
immunoassays

Antibody Pairs•	Specific for the gamma subunit without detectable crossreactivity with the alpha or beta subunits• Suitable for use in ELISA• MAbs produced in vivo
CAPTURE
DETECTION
M86520M
M86406M
M86416M
M86141M
M86101M

M86101M
M86101M
M86101M
M86201M
M86520M

Antigens
A86803H
				• Native antigen from human brain• > 95% pure (SDS-PAGE)• Suitable for use in ELISA and WB

A01359H
				• Recombinant (E. coli), a.a. 2-434• > 95% pure (SDS-PAGE)• Suitable for use in ELISA, LF, WB, DB and IHC

A01448H
				• Recombinant (E. coli), a.a. 1-434• 95% pure (SDS-PAGE)• Suitable for use in ELISA

Neutrophil Gelatinase-AssociatedLipocalin (NGAL) / Lipocalin-2
A glycoprotein involved in the regulation of cellular apoptosis andthe transport of iron across cell membranes. It has recently been
implicated in atherosclerosis through its effects on MMP-9, an
important mediator of vascular remodelling and plaque instability.
Increased levels of NGAL have been found in patients withatherosclerotic plaques and myocardial infarction.

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

K01392C

C. Eggs

Aff.Pur.

N/A

Antigens
9278
		
		

• Recombinant (CHO cells)• 95% pure (SDS-PAGE)• Suitable for use in WB

www.MeridianLifeScience.com
www.MeridianLifeScience.com |1515

Product list | Continued
Plasminogen Activator Inhibitor 1 (PAI-1)
PAI-1 is the principal inhibitor of tissue plasminogen activator(tPA) and urokinase Plasminogen Activator (uPA), the activatorsof plasminogen and fibrinolysis. Increased PAI-1 levels may
predispose patients to the formation of atherosclerosis plaques.
Clinical evidence suggests that increased PAI-1 levels areassociated with atherothrombosis and elevated plasma PAI-1
levels have been identified as a predictor of myocardial infarction.

Monoclonals• Recognizes both free and complexed forms of Human PAI-1• Suitable for use in ELISA• Produced in vivo
N63913M

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

K63132R

RabbitPurified

EIA,IHC,WB

Antigens
A63625M
		• Recombinant (E. coli)• >95% pure (SDS-PAGE), 80 ± 5% active byuPA titration

Pregnancy Associated Plasma Protein A(PAPP-A)
PAPP-A is a high molecular weight, zinc-binding metalloproteinasethat is associated with vulnerable plaques and may predict CVD
and mortality. Human PAPP-A antigen purified from placental
blood is a heterotetrameric complex (htPAPP-A) consisting of two
PAPP-A subunits and two proMBP subunits. Human PAPP-A
antigen purified from therosclerotic plaques is homodimeric(dPAPP-A) consisting of two PAPP-A subunits.

Monoclonals•	Detects heterotetrameric PAPP-A (htPAPPA), unless
noted otherwise• Suitable for use in ELISA• MAbs produced in vivo
E86509M
E86509M
E01261M

1616| Cardiac
Cardiac && Metabolic
Metabolic Markers
Markers forfor Assay
Assay Development
Development

Antibody Pairs•	Detects heterotetrameric PAPP-A (htPAPPA), unless
noted otherwise• Suitable for use in ELISA• MAbs produced in vivo
CAPTURE
DETECTION
E86509M
E86141M
E01345M
E01343M

E01261M
E86910M
E01344M
E01261M* Pair also detects dimeric PAPP-A* Pair detects dimeric PAPP-A only
* Pair detects dimeric PAPP-A only

Antigens
A86864H
				
		
A01684H
						• Native antigen sourced from pooled humanretroplacental blood• Specific for heterotrimeric complex (PAPP-A
and pro-MBP subunits)• Suitable for use in IFA• > 85% pure (SDS-PAGE), lyophilized• Recombinant (human cell line)• Specific for homodimeric form (dPAPP-A)• Suitable for use as a standard in ELISA or for
antibody production• > 90% pure (SDS-PAGE)

Procalcitonin (PCT)
PCT is a peptide precursor of the hormone calcitonin and has
shown promise as a diagnostic marker of acute inflammatory
conditions. Specifically it has been implicated as an inflammation
marker in early atherosclerosis and in bacterial infections.

Monoclonals• Suitable for use in ELISA and WB (unless otherwise noted)• Produced in vivo
E01308M* Biotin conjugatedE86131M* Also detects calcitonin

Antibody Pairs• Suitable for use in ELISA and WB• MAbs produced in vivo
CAPTURE
DETECTION
E86561M
E86561M
E86813M
E01291M
E01341M
E01342M
E86412M
E86813M

E86494M
E86420M
E86112M
E86494M
E01340M
E01340M
E86813M
E86112M* Also works in LF assays* Also detects katacalcin* Also detects katacalcin

Antibody Pairs

Procalcitonin (PCT) continued
CAPTURE

DETECTION

E01342M

E01340M* Also detects katacalcin and works
in LF assays

Antigens
A01454H
						• Recombinant (E. coli)• Represents a.a. 1-116, contains no tags
• > 95% pure, lyophilized• Suitable for use in ELISA

A01367H
						• Recombinant (E. coli)• Represents a.a. 3-116, contains a His-tag• > 95% pure (SDS-PAGE)• Suitable for use in ELISA and WB

Procollagen Type III
Procollagen Type III is a precursor molecule of Collagen Type
III, a fibrillar collagen that is found in connective tissues suchas skin, lung, uterus, intestine and the vascular system, usuallyin association with type I collagen. Increased serum levels of
Procollagen Type III have been associated with atherosclerosis.

• Does not cross react with human C-peptide• Suitable for use in ELISA• MAbs produced in vivo
CAPTURE
DETECTION
E86209M
E86104M
E01339M
E01339M

E86102M
E86210M
E83102M
E86802M* E86102M recognizes human insulin* E83102M recognizes human insulin

RelaxinRelaxin is a peptide hormone that has a variety of actions on
reproductive and non-reproductive organs. Recent research
has found that relaxin levels are increased in heart failure andcorrelates with its severity. Relaxin has also been evaluated asa pharmacologic agent for the treatment of patients with acuteheart failure.

Monoclonals• Suitable for use in ELISA and FC• Produced in cell culture
E24520M* Specific for Relaxin 1 and does not cross-react with
Relaxin 2

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

T40331R

RabbitPurified

EIA,IFA,IHC,RIA

Proinsulin
Proinsulin is the prohormone precursor to insulin. In later stagesof type 2 diabetes, proinsulin and proinsulin-like molecules are
secreted in increasing amounts with insulin. Studies haveshown elevated intact proinsulin seems to indicate an advanced
stage of ß-cell exhaustion and is a highly specific marker for
insulin resistance.

Monoclonals• Does not cross react with human C-peptide• Suitable for use in ELISA• MAbs produced in vivo
E83802M• Does not cross react with human C-peptide• Suitable for use in ELISA• MAbs produced in vivo
• Recognizes human insulin
E83102M

E24720M* Recognizes Relaxin 1 and 2

Renin
Renin is an enzyme released by the kidneys and is part of therenin-angiotensin system, a group of related hormones that act
together to regulate blood pressure. A renin assay blood test is
performed to find the cause of high blood pressure(hypertension). It is often tested alongside aldosterone (ahormone made by the adrenal glands) in order to obtain acomplete understanding of an individual’s hormone balance.

Antibody Pairs• Suitable for use in ELISA• Produced in cell culture
CAPTURE
DETECTION
E01363M
E01365M
E01365M
E01366M
E01366M

E01364M
E01363M
E01364M
E01364M
E01363M

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www.MeridianLifeScience.com |1717

Product list | Continued
S-100

Serum Amyloid A (SAA)The S-100 family of proteins are involved in the regulation of anumber of cellular processes including cell cycle progression anddifferentiation. Studies have shown that serum measurementsof S100-beta maybe useful for the diagnosis and prognosis ofacute stroke.

SAA is a sensitive marker of acute inflammation that has
been implicated in several chronic inflammatory diseases
such as amyloidosis, atherosclerosis, and rheumatoid arthritis.
A systemic inflammation (manifested by high SAA levels) may
promote atherosclerotic plaque destabilization, in addition toexerting a possible direct effect on atherogenesis.

Monoclonals• Produced in vivo
MEM24-211 * Works in IHC
Q86403M* Recognizes S-100 (beta-beta) and S-100 (alpha-beta), works in WB

Antibody Pairs• Suitable for use in ELISA and IP• MAbs produced in vivo
CAPTURE
DETECTION
Q86006M

Q86610M* Specific for S-100 beta-beta andalpha-beta

Q86003M

Q86610M* Specific for S-100 beta-beta andalpha-beta

Antigens
A86809H
						
A86289H				Antibody Pairs• Suitable for use in ELISA and WB• Recognizes native and recombinant human SAA• MAbs produced in vivo
CAPTURE
DETECTION
H01381M
H01382M
H86177M
H86180M
H86177M
H86178M

H01383M
H01384M
H86180M
H86177M
H86178M
H86177M

Polyclonals• Native antigen from human brain• Contains beta-beta homodimer (S-100b) andalpha-beta heterodimer (S-100a)• > 95% pure, lyophilized• Suitable for ELISA, as an immunogen for
antiserum production and tracer for iodination• Native S-100b from human brain• > 95% pure, lyophilized• Suitable for ELISA, as an immunogen for
antiserum production and tracer for iodination

S-Adenosyl-Homocysteine (SAH)
SAH is formed by the demethylation of S-adenosyl-L-methionine(SAM) and acts as an intermediate in the synthesis of cysteine
and adenosine. It is elevated in CVD and serves as anindicator of various diseases involving vascular dysfunction.

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

K01395C

C. Eggs

Aff.Pur.

N/A

1818| Cardiac
Cardiac && Metabolic
Metabolic Markers
Markers forfor Assay
Assay Development
Development

CATALOG

SOURCEFORMATAPPLICATION

H01379R

RabbitPurified

EIA, IEP

Antigens
R01619				• Recombinant (E. coli)• 122 a.a. long with N-terminal His tag• Suitable for use in ELISA

Stromal Cell-Derived Factor 1 alpha (SDF-1α)
SDF-1α is a chemokine produced in two forms, SDF-1α andSDF-1β by alternate splicing of the same gene. Studies have foundincreased levels of SDF-1α in several cardiac diseases. It has
been suggested to be a prognostic marker in atrial fibrillation.

Antigen
A42028H
		• Recombinant (E. coli)• >98% pure (RP-HPLC and SDS-PAGE),
lyophilizedThrombomodulin

Troponin C-Cardiac (cTnC)

Thrombomodulin is a protein present in the endothelial
membrane of cells which serves as a co-factor to protein C in the
coagulation cascade. Its functions as an anti-coagulant regulator,and can be measured as a soluble protein in the serum after
vascular injury.

cTnC is part of the Troponin Complex and is responsible forbinding calcium to activate muscle contraction. In cardiac
muscle, cTnC binds to Cardiac Troponin I (cTnI) and Cardiac
Troponin T (cTnT), whereas cTnC binds to slow Skeletal Troponin
I (ssTnI) and Troponin T (ssTnT) in slow-twitch skeletal muscle.

Antigens

Antigens

R01802						• Human Thrombomodulin (THBD) Protein,
Recombinant (His-tag C-terminus)• Source: HEK293)• ≥ 90% (SDS-PAGE) in phosphate buffer
saline pH 7.2• Suitable for ELISA, lateral flow, and western blot

A86857H
				• Native antigen from human heart tissue• > 98% pure (SDS-PAGE), lyophilized• Suitable for use as a standard, immunogen for
antisera production, tracer for iodination

Troponin I-Cardiac (cTnI)

CATALOG

SOURCEFORMATAPPLICATION

As part of the troponin complex, cTnI binds to actin in thin
myofilaments to hold the actin-tropomyosin complex in place andis present in cardiac muscle tissue in a single isoform. cTnI has
several phosphorylation sites and the pattern of phosphorylation
changes in response to disease. Phosphorylated cTnI changes
the conformation of the protein and modifies its interaction withother troponins and alters the myofilament response to calcium.
Assays for Cardiac Troponins I and T (cTnI and cTnT) have become
widely accepted tools for diagnosing acute myocardial infarction.

K63250R

RabbitPurified

EIA,IHC,WB

Monoclonals

Tissue Plasminogen Activator (tPA)tPA is a serine protease that catalyzes the conversion ofplasminogen to plasmin, the major enzyme responsible for clot
breakdown. An elevated level of tPA antigen is a proposed
marker of increased coronary risk.

Polyclonals

Troponin Complex
Troponin is a complex of three regulatory proteins (Troponin C,
Troponin I, and Troponin T) that is integral to muscle contraction
in skeletal muscle and cardiac muscle, but not smooth muscle.
Troponin I and T are specific to cardiac muscles and are very
sensitive and specific markers for unstable angina and myocardial
infarction. Troponin C is associated with both cardiac and skeletal
muscles and is not used in the diagnosis of myocardial damage.

Antigens
A86608H
		• Native antigen from human heart tissue• >95% pure (SDS-PAGE)

A86111H		• Native antigen from human heart tissue• Complex of Troponin I-T-C in a molar ratio 1:1:1• Suitable for use in ELISA and WB•	Reacts equally with free cardiac Troponin I (cTnI) andComplexed cTnI (unless otherwise noted)• Not affected by phosphorylation• Produced in vivo
		
EPITOPE BINDING
H01250M
a.a. 1-15* Also works in IHC
H86008M
a.a. 18-28* Also works in IHC
H01247M
a.a. 148-158 * Also works in IHC, reacts mostly with free cTnI(not complexed)

H86267M
H86550M
H86004M
H86307M
H01340M
H86207B
H01246M

a.a. 169-178

H86286M
H86241M
H86596M

a.a. 186-192
a.a. 190-196* Also works in IHCa.a. 25-40
a.a. 41-49
a.a. 41-49* Biotin conjugateda.a. 65-74* Also works in IHC, reacts with free cTnI only
(not complexed)

a.a. 86-90* Also works in IHCa.a. 86-90
a.a. 169-178* Also works in IHC

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Product list | Continued
Troponin I-Cardiac (cTnI) continued

Troponin I-Skeletal (sTnI)

Antibody Pairs

The Troponin I subunit sTnI exists in three separate isoforms,
two in skeletal muscle fibers and one in cardiac muscle (cTnI).
Diagnosis of acute myocardial infarction by Troponin I isdependent on the detection of cTnI only (i.e. the assay should
not be cross-reactive to Troponin I-skeletal). Antibodies madeagainst this cardiac isoform are immunologically different fromantibodies made against the two skeletal isoforms.

• Suitable for use in ELISA• MAbs produced in vivo (unless otherwise noted)
CAPTURE
DETECTION
H86465M

H86458M* Also works in WB and IP, nocross-reactivity with skeletal
muscle troponin I

K31015M

K31341G* K31341G is a polyclonal (goat) cTnI,
also works in IHC

K31015M

K31342G* K31342G is a polyclonal (goat) cTnI,
also works in IHC

H01326M
H86285M
H01395M
H01395M
H01395M
H86625M
H01395M
H01340M

H01325M
H01347M
H01343M
H86465M
H86280M
H01395M
H01396M
H01396M* Also works in LF* Also works in WB* Also works in WB* Also works in WB* Also works in WB• Cross-reactivity with Cardiac Troponin I is < 1.5%
• Suitable for use in ELISA, WB and for immunopurification• MAbs produced in vivo
CAPTURE
DETECTION
H86121M

CATALOG

SOURCEFORMATAPPLICATION

K01350G
K31342G

Goat
Goat

Aff.Pur.
Aff.Pur.

EIA
EIA,IHC* K31342G reacts with region a.a. 69-86

Antigens
R01620				• Recombinant (E. coli)• 95% pure (SDS-PAGE Coomassie staining)• Suitable for use in ELISA

A86853H
				• Native antigen from human heart• >98% pure (SDS-PAGE)• Suitable for use in ELISA, as antigen for antisera
production, and as a tracer for iodination

Antigens
A86824H
		• Native antigen from human skeletal muscle• >95% pure (SDS-PAGE), lyophilized• Native antigen from human heart• >98% pure (SDS-PAGE)

TnT is a tropomyosin-binding subunit which regulates theinteraction of Troponin Complex with thin myo-filaments. Two
isoforms of TnT are expressed in human skeletal muscle tissue(sTnI and sTnT) and several isoforms are expressed in cardiac
specific tissue (cTnT). Although assays for Cardiac Troponin T(cTnT) and Cardiac Troponin I (cTnI) exhibit similar clinicalperformance in patients with acute coronary syndromes fordiagnosis and risk stratification, there are differences in therelease and clearance of these proteins from damaged myocytes.
Also cTNT has a higher overall tissue concentration and freecytoplasmic concentrations than cTnI, and appears in blood ofpatients with acute myocardial infarction as a mixture ofcomplexed (cTnT-I-C) and free cTnT.

Antibody Pairs• No cross-reactivity with human skeletal Troponin T• Suitable for use in ELISA and WB• MAbs produced in vivo
CAPTURE
DETECTION
H86111M
H86111M
H86111M
H86429M
H86429M
H86429M

20

H86702M

Troponin T-Cardiac (cTnT)Polyclonals

A38150H
		

Antibody Pairs

Cardiac & Metabolic Markers for Assay Development

H01363M
H86707M
H86906M
H86906M
H86111M
H86519M

Troponin T-Cardiac (cTnT) continued

Vitamin D

Monoclonals

Vitamin D is a fat-soluble precursor of the steroid hormone
calcitriol that is essential for bone health and mineral metabolism.
Total Vitamin D assays measure the level of both 25 OH Vitamin
D3 (calcidiol) and 1,25-Dihydroxy Vitamin D3 (calcitriol). Serum 25
OH Vitamin D3 is the most stable circulating form of vitamin D
and a low blood level suggests that a person is not getting
enough exposure to sunlight or enough dietary vitamin D.
1,25-Dihydroxy Vitamin D3 regulates the expression of hundredsof genes and a low level is indicative of kidney failure.

• Suitable for use in ELISA and IP• Not cross-reactive with skeletal Troponin T• Produced in vivo
		
EPITOPE BINDING
H86914M
a.a. 146-160

Antigens
A86813H
				• Native antigen from human heart• >98% pure (SDS-PAGE)• Suitable for use in ELISA, as antigen for antisera
production, and as a tracer for iodination

Antibody and Antigen Pairs
ANTIBODYANTIGEN

K01214M
K01214M

A01697B
A01698B*Lateral Flow
*ELISA and RIA

Tumor Necrosis Factor alpha (TNF-alpha)Monoclonals

TNF-alpha is a cytokine involved in systemic inflammation andimplicated in post-ischemic myocardial dysfunction and heart
failure. Concentrations of TNF-alpha reflect the impairment ofcardiac and renal function in patients with CAD. Metabolic
syndrome and diabetes are also associated with higher plasma
concentrations of TNF-alpha and its receptors.

•	Suitable for use in ELISA• Produced in cell culture
K24123M		*MAb to 1,25 (OH)2 Vitamin D3
K24124M		*MAb to 25-OH Vitamin D3 Lyophilized
K24124M-LQ		 *MAb to 25-OH Vitamin D3 Liquid

Antibody Pairs• Suitable for use in ELISA and IHC•	Inhibits the biological activity of TNF-alpha in various
biological fluids
CAPTURE
DETECTION
H86410M

H86650M

Antigens
A01694D		• 99% Pure (HPLC)• 25-OH Vitamin D3 BSA Conjugate

A01409H
		• > 99% Pure (HPLC)• 1,25-Dihydroxy Vitamin D3

A50674H
		• > 95% Pure (SDS-PAGE)• Vitamin D Binding Protein (GC Globulin)

Uroguanylin
Uroguanylin is a novel natriuretic hormone system andparticipates in the regulation of salt and water homeostasis. It issynthesized as prohormone and requires proteolytic processing
similar to the other natriuretic peptides. It is also activated inheart failure, but acts on a different cyclic GMP pathway to ANP
and BNP.

Polyclonals
CATALOG

SOURCEFORMATAPPLICATION

D01240R

RabbitNeat

EIA,IHC,RIA,WB* D01240R is specific for the circulating form

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Meridian Life Science, Inc.
5171 Wilfong Road | Memphis, TN 38134
901.382.8716 • 800.327.6299
www.MeridianLifeScience.com

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