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第106回アメリカ歯周病学会共催
日本歯周病学会・日本臨床歯周病学会・2020年大会

The 106th Annual Meeting of the American Academy of Periodontology
in collaboration with the Japanese Society of Periodontology,
the Japanese Academy of Clinical Periodontology.

JSP/JACP ポスターセッション抄録集
一般演題（基礎研究，臨床研究），症例報告，歯科衛生演題

Abstracts of JSP/JACP Poster Session
General (Basic Research, Clinical Research), Case Report, Dental Hygiene

会期：2020年11月 6 日〜11月15日
会場：オンライン開催

November 6 ‑15, 2020
Virtual第106回アメリカ歯周病学会共催
日本歯周病学会・日本臨床歯周病学会 2020年大会
JSP/JACP ポスター発表・若手研究者支援協賛企業

相田化学工業株式会社
医歯薬出版株式会社
科研製薬株式会社
ストローマン・ジャパン株式会社
株式会社デンタリード
株式会社トクヤマデンタル
プレミアモード株式会社（50音順）

General（Basic Research）

1

GB‑01

(൵HFWVRIDJLQJLQ/36‑LQGXFHGFKURQLFSHULRGRQWLWLVPRXVHPRGHO
Hirotsugu Morinaga＊, Keiichi Kanayama, Toru Hasegawa,Takumi Sato, Mitsunobu Kitago, Jyunichi Tatsumi
Department of Periodontology Asahi Unversity of Dentistry
Background and objective: $OYHRODUERQHUHVRUSWLRQGXHWRFKURQLFLQÀDPPDWLRQLVWKHPDLQV\PSWRPRISHULRGRQWLWLV&RQYHQWLRQDOPRXVHSHULRGRQWLWLVPRGHOVUHTXLUHLPSURYHPHQWDVDPRGHORIFKURQLFLQÀDPPDWLRQSDUWLFXODUO\ZLWKUHVSHFWWRWKHPHWKRGRILQGXFLQJLQÀDPPDWLRQGXULQJWKHREVHUYDWLRQSHULRG,QWKLVVWXG\DOYHRODUERQHUHVRUSWLRQZDVLQGXFHGE\DPHWKRGZKLFKFDXVHGOLWWOHPHFKDQLFDOLUULWDWLRQDQGSHULRGRQWLWLVSURJUHVVLRQLQ\RXQJDQGROGPLFHZDVFRPSDUHG
Materials and Methods: Lipopolysaccharide（LPS）from Porphyromonas gingivalis was administered 12 times（twice perweek）on the palatal gingiva of 8‑ or 24‑ZHHNVROG&%/-PLFH7KH/36ZDVLQMHFWHGE\DPLFURV\ULQJH（33 G）, andthe dose was 20 ȝJSHUVKRW0D[LOODVDPSOHVZHUHFROOHFWHGZHHNDQGZHHNVDIWHUWKH¿QDODGPLQLVWUDWLRQDQGPLFUR‑&7VFDQVDQGWLVVXHVOLFHVZHUHSUHSDUHG
Results: ,QMHFWLRQRI/36E\WKHPLFURV\ULQJHGLGQRWUHTXLUHVWURQJH[WHUQDOIRUFH5HVXOWVIURPWKHPLFUR‑CT showed that
ERQHUHVRUSWLRQVWLPXODWHGE\/36ZDVJUHDWHULQWKHROGHUPLFHWKDQLQWKH\RXQJPLFH,QWKHFRPSDULVRQEHWZHHQZHHNDQGZHHNVDIWHUWKH¿QDODGPLQLVWUDWLRQWKHDJHGPLFHGHPRQVWUDWHGPRUHERQHUHVRUSWLRQODWHUWKDQWKH\RXQJPLFHLQDIWHUZHHNV
Conclusion: LPS administration with a micro‑V\ULQJHFDXVHGDOYHRODUERQHUHVRUSWLRQLQERWK\RXQJDQGROGPLFH,QROGHU
PLFHERQHUHVRUSWLRQZDVREVHUYHGFRQWLQXRXVO\LQZHHNVDIWHU/36DGPLQLVWUDWLRQGB‑02([RVRPHVIURPGHQWDOSXOSFHOOVFDQVXSSUHVVSHULRGRQWLWLVV\PSWRPV
Yuta Shimizu＊1, Tomoko Kawaguchi2+LURWDND,NDPL1, Tadashi Yasuda1,
Toshiyuki Shibata2, Ken‑ichi Tezuka3, Junichi Tatsumi1
1Asahi University School of Dentistry Department of Periodontology, 2Gifu University Graduate School of
0HGLFLQH'HSDUWPHQWRI2UDODQG0D[LOORIDFLDO6FLHQFH3Gifu University Graduate School of Medicine'HSDUWPHQWRI7LVVXHDQG2UJDQ'HYHORSPHQW5HJHQHUDWLRQDQG$GYDQFHG0HGLFDO6FLHQFH

Background and objective: ([WUDFHOOXODUYHVLFOHVFDOOHGH[RVRPHVDUHVHFUHWHGIURPYDULRXVFHOOW\SHVDQGVHUYHDVDWRRO
LQLQWHUFHOOXODUFRPPXQLFDWLRQV:HKDYHSUHYLRXVO\HVWDEOLVKHGDQGFKDUDFWHUL]HGKXPDQOHXNRF\WHDQWLJHQ（HLA）
haplotype homo（HHH）dental pulp cell lines（DPCs）IURPKXPDQWKLUGPRODUWHHWK,QWKLVVWXG\H[RVRPHVVHFUHWHG
from HHH‑'3&VZHUHLQMHFWHGLQWRPLFHZLWKSHULRGRQWDOGLVHDVHDQGWKHLULQKLELWRU\HIIHFWIRUSHULRGRQWDOGLVHDVHSURJUHVVLRQZDVHYDOXDWHG
Materials and Methods: )RUH[RVRPHVSXUL¿HGIURP+++‑'3&VZHFRQ¿UPHGWKHSDUWLFOHVL]HH[RVRPHPDUNHUDQG
+/$FODVV,H[SUHVVLRQDQGDQDO\]HGPL51$H[SUHVVLRQSURILOHVRIERWK+++‑'3&VDQGH[RVRPHV)XUWKHUPRUHZH
evaluated the cell migration and proliferation potential of DPCs using HHH‑'3& H[RVRPHV:H DOVR H[DPLQHG WKHVXSSUHVVLRQH൵HFWRI+++‑'3&H[RVRPHVRQSHULRGRQWDOGLVHDVHSURJUHVVLRQFDXVHGE\VLONOLJDWLRQResults: HHH‑'3&H[RVRPHVKDGDKRPRJHQHRXVDQGVSKHULFDOPHPEUDQHVWUXFWXUHDQGDVFRPSDUHGWR+++‑DPCs, theyVKRZHGORZHUH[SUHVVLRQRI+/$FODVV,+++‑'3&H[RVRPHVSURPRWHGWKHPLJUDWLRQDQGSUROLIHUDWLRQRI'3&VDQGWKH
PL51$ H[SUHVVLRQ SURILOH RI +++‑'3& H[RVRPHV ZDV VLPLODU WR WKDW RI WKH RULJLQDO FHOOV ,Q DGGLWLRQ PLFH ZLWKSHULRGRQWDOGLVHDVHVKRZHGDQLQKLELWRU\H൵HFWRQDOYHRODUERQHUHVRUSWLRQ
Conclusion: ([RVRPHVSURPRWHPLJUDWLRQDQGSUROLIHUDWLRQRI'3&VDQGH[KLELWDQDOYHRODUERQHUHVRUSWLRQLQKLELWRU\HIIHFW LQ PLFH ZLWK SHULRGRQWDO GLVHDVH7KLV VXJJHVWV WKDW +++‑'3& H[RVRPHV PD\ EH XVHG LQ WKH WUHDWPHQW RI
SHULRGRQWLWLVZLWKRXWFHOOWUDQVSODQWDWLRQ

JSP/JACP Poster Session AAP2020

2

GB‑03

(൵HFWVRIGHVLJQHUVHOI‑DVVHPEOLQJSHSWLGHK\GURJHOVRQSHULRGRQWDOGHIHFWKHDOLQJ
Daisuke Matsugami＊1,2, Tasuku Murakami1:DWDUX<RVKLGD1,.HQWDUR,PDPXUD1, Fumi Seshima1, Atsushi Saito1, 2
1

Department of Periodontology, Tokyo Dental College, 2Oral Health Science Center, Tokyo Dental College

Background and objective: :HSUHYLRXVO\UHSRUWHGWKDWWKHORFDODSSOLFDWLRQRIVHOI‑assembling peptide（SAP）QDQR¿EHU
K\GURJHO5$'$SURPRWHVSHULRGRQWDOKHDOLQJLQYLYR5$'$FDQEHIXQFWLRQDOL]HGE\FHOODGKHVLRQPRWLIV7KLVVWXG\DLPHGWRLQYHVWLJDWHWKHH൵HFWVRIWKHORFDODSSOLFDWLRQRIWZRGHVLJQHU6$3K\GURJHOV35*（2‑XQLW5*'ELQGLQJ
sequence）and PDS（laminin cell adhesion motif）
RQWKHKHDOLQJRIVXUJLFDOSHULRGRQWDOGHIHFWVLQUDWV
Materials and Methods: ,QYLWURUDWSHULRGRQWDOOLJDPHQW‑derived cells were cultured, seeded on each gel, and analyzed bycell proliferation assay（:67‑1） ,Q YLYR VWDQGDUGL]HG SHULRGRQWDO GHIHFWV ZHUH VXUJLFDOO\ FUHDWHG PHVLDOO\ LQ WKHPD[LOODU\ ILUVW PRODUV RI UDWV 'HIHFWV ZHUH WUHDWHG ZLWK 5$'$ 35* 3'6 RU OHIW XQILOOHG$IWHU  DQG  ZHHNV
postoperatively, healing of periodontal defects was assessed by microcomputed tomography, histological and
LPPXQRKLVWRFKHPLFDODQDO\VHV
Results: Proliferation of PDL‑GHULYHGFHOOVLQ35*JURXSZDVVLJQL¿FDQWO\JUHDWHUWKDQWKDW5$'$DQG3'6JURXSV（p＜）DWKRXUV$WZHHNVSRVWRSHUDWLYHO\WKHERQHYROXPHIUDFWLRQVLQ35*JURXS（±％）and PDS group（±％）ZHUH VLJQLILFDQWO\ JUHDWHU WKDQ 5$'$ JURXS（±％）（p＜） +LVWRORJLFDOO\ QHZ ERQH
IRUPDWLRQZDVPRUHSURQRXQFHGLQ35*DQG3'6JURXSVFRPSDUHGWR5$'$JURXSDWZHHNVSRVWRSHUDWLYHO\,Q35*
JURXSLQFUHDVHGSURSRUWLRQVRI3&1$‑ and VEGF‑SRVLWLYHFHOOVZHUHREVHUYHGQHDUWKHQHZO\IRUPHGERQHDGMDFHQWWRWKH
root at 4 weeks postoperatively（p＜）
Conclusion: 7KHUHVXOWVRIWKLVVWXG\VXJJHVWWKDWGHVLJQHU6$3K\GURJHOVHVSHFLDOO\35*HQKDQFHGSHULRGRQWDOKHDOLQJE\
LQFUHDVLQJFHOOSUROLIHUDWLRQDQGDQJLRJHQHVLV

GB‑04(൵HFWRI&7/$‑RQERQHUHVRUSWLRQLQSHULRGRQWLWLVPRGHOPRXVH
6DNL1DNDQH＊1.HQWDUR,PDPXUD1, Eiichi Suzuki1.D]X\XNL,VKLKDUD2,3, Atsushi Saito1,2
1

Department of Periodontology, Tokyo Dental College, 2Oral Health Science Center, Tokyo Dental College,3
Department of Microbiology, Tokyo Dental College

Background and objective: CTLA‑LVRQHRIWKHLPPXQHFKHFNSRLQWUHFHSWRUVH[SUHVVHGRQWKHFHOOVXUIDFHRI7FHOOV,W
has been reported that CTLA‑PD\VXSSUHVVERQHUHVRUSWLRQLQUKHXPDWRLGDUWKULWLV+RZHYHULWVH൵HFWRQDOYHRODUERQHUHVRUSWLRQKDVQRWEHHQFODULILHG7KHSXUSRVHRIWKLVVWXG\LVWRLQYHVWLJDWHWKHUROHRI&7/$‑4 in bone resorption in
SHULRGRQWLWLV
Materials and Methods: Periodontitis was induced by silk ligature to the second molar in C57BL/6 mice, CTLA‑4‑,JZDVDGPLQLVWHUHGLQWUDSHULWRQHDOO\7KHHIIHFWRI&7/$‑4‑,JRQDOYHRODUERQHUHVRUSWLRQDQGRVWHRFODVWGLIIHUHQWLDWLRQZDVHYDOXDWHGE\&7DQG75$3VWDLQLQJ,QYLWUR5DZFHOOVZHUHWUHDWHGZLWK5$1./DQG&7/$‑4‑,JDQGWKHUROHRI
CTLA‑4‑,JRQWKHRVWHRFODVWGLIIHUHQWLDWLRQZDVHYDOXDWHG7KHH[SUHVVLRQOHYHOVRIRVWHRFODVWGLIIHUHQWLDWLRQPDUNHUV（c‑IPV&DUERQLFDQK\GUDVH,,DQG&DWKHSVLQ.）and signaling molecule downstream of CTLA‑4（PPA2）were assessed
E\T57‑3&57KHQXPEHURIRVWHRFODVWVZDVFRXQWHGE\75$3VWDLQLQJResults: Bone resorption and the number of osteoclast‑like cells were decreased by administration of CTLA‑4‑,J LQ
SHULRGRQWLWLV PRGHO PLFH ,Q YLWUR &7/$‑4‑,J WUHDWPHQW VLJQLILFDQWO\ UHGXFHG P51$ H[SUHVVLRQ RI RVWHRFODVWGL൵HUHQWLDWLRQPDUNHUVDQGWKHQXPEHURIRVWHRFODVW‑OLNHFHOOV,QDGGLWLRQWKHH[SUHVVLRQOHYHORI33$ZDVLQFUHDVHGE\
CTLA‑4‑,JWUHDWPHQWLQDFRQFHQWUDWLRQ‑GHSHQGHQWPDQQHU
Conclusion: Our data showed that administration of CTLA‑4‑,JUHGXFHGWKHERQHUHVRUSWLRQDQGWKHQXPEHURIRVWHRFODVWV,QDGGLWLRQWKHH[SUHVVLRQRI33$ZDVLQFUHDVHGE\&7/$‑4‑,J,QFRQFOXVLRQLWLVVXJJHVWHGWKDW&7/$‑4‑,JUHGXFHVDOYHRODUERQHUHVRUSWLRQWKURXJKWKHLQKLELWLRQRIRVWHRFODVWGL൵HUHQWLDWLRQYLDDFWLYDWLRQRI33$DWORFDOSHULRGRQWLWLVVLWH

JSP/JACP Poster Session AAP2020

3

GB‑05

&RPELQHGH൵HFWVRI)*)‑DQG'%%0RQSHULRGRQWDOKHDOLQJDSUHFOLQLFDOLQYHVWLJDWLRQ
Tasuku Murakami＊1, Daisuke Matsugami1,2:DWDUX<RVKLGD17DNDKLUR%L]HQMLPD1,.HQWDUR,PDPXUD1, Fumi Seshima1, Atsushi Saito1,2
1

Department of periodontology, Tokyo Dental College,
2
Oral Health Science Center, Tokyo Dental College

Background and objective: Fibroblast growth factor（FGF）‑2 has been reported to promote periodontal healing and used
IRUSHULRGRQWDOUHJHQHUDWLRQWKHUDS\'HSURWHLQL]HGERYLQHERQHPLQHUDO（DBBM）has osteoconductivity and has been
FOLQLFDOO\DSSOLHGDVDVFDIIROGPDWHULDO+RZHYHULQIRUPDWLRQLVOLPLWHGUHJDUGLQJWKHFRPELQHGHIIHFWVRI)*)‑2 and'%%0RQSHULRGRQWDOKHDOLQJ7KHSXUSRVHRIWKLVVWXG\LVWRLQYHVWLJDWHWKHHIIHFWVRIWKHFRPELQDWLRQRI)*)‑2 and'%%0RQWKHKHDOLQJRIVXUJLFDOO\FUHDWHGSHULRGRQWDOGHIHFWVLQUDWV
Materials and Methods: 3HULRGRQWDOGHIHFWVZHUHFUHDWHGPHVLDOO\WRWKHPD[LOODU\¿UVWPRODUVLQ:LVWDUUDWV'HIHFWVZHUH
¿OOHGZLWK)*)‑2, DBBM, FGF‑'%%0RUOHIWXQ¿OOHG0LFURFRPSXWHGWRPRJUDSK\KLVWRORJLFDO（H&E staining）, andimmunohistochemical（3&1$ VWDLQLQJ）H[DPLQDWLRQV ZHUH XVHG WR HYDOXDWH SHULRGRQWDO KHDOLQJ DW  RU  ZHHNVSRVWRSHUDWLYHO\Results: At 2 weeks postoperatively, bone volume fraction was significantly greater in the FGF‑2 and FGF‑2+DBBMJURXSVWKDQWKDWLQWKH8Q¿OOHGDQG'%%0JURXSV$WZHHNVSRVWRSHUDWLYHO\ERQHYROXPHIUDFWLRQZDVVLJQL¿FDQWO\greater in FGF‑2, DBBM, and FGF‑'%%0JURXSVWKDQWKDWLQWKH8Q¿OOHGJURXS+LVWRORJLFDODVVHVVPHQWVKRZHGWKDWnewly formed bone in the FGF‑2 and FGF‑'%%0JURXSVDSSHDUHGWREHJUHDWHUWKDQLQWKH8Q¿OOHGJURXS3URSRUWLRQV
RI3&1$‑positive cells above newly formed bone in the FGF‑2, DBBM, and FGF‑'%%0JURXSVZHUHVLJQL¿FDQWO\JUHDWHUWKDQWKH8Q¿OOHGJURXSDWZHHNV
Conclusion: 7KHVH¿QGLQJVVXJJHVWWKDWWKHFRPELQDWLRQRI)*)‑2 and DBBM enhances bone formation in the treatment of
SHULRGRQWDOGHIHFWVGB‑06

4XDQWLWDWLYHFKDQJHVRIPorphyromonas gingivalisDQG(%9'1$LQVDOLYDEHIRUHDQGDIWHULQLWLDOSHULRGRQWDOWKHUDS\LQ-DSDQHVHSHULRGRQWLWLVSDWLHQWVYorimasa Ogata＊1, Ayako Kato1.HQLFKL,PDL2<RULQREX,NHGD1
1'HSDUWPHQWRI3HULRGRQWRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\DW0DWVXGR2'HSDUWPHQWRI0LFURELRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\

Background and objective: 5HFHQWO\ (SVWHLQ‑Barr virus（EBV）is thought to be involved in the pathogenesis ofperiodontitis as well as Porphyromonas gingivalisZKLFKLVWKHUHSUHVHQWDWLYHSHULRGRQWRSDWKLFEDFWHULD7KHSXUSRVHRI
initial periodontal therapy（,37）is to enhance motivation and remove calculus, periodontopathic bacteria and their
E\SURGXFWVLQRUGHUWRUHVWRUHSHULRGRQWDOKHDOWK7RHOXFLGDWHWKHH൵HFWVRI,37RQLQFLGHQFHRIP. gingivalis and EBV'1$ZHXVHGZKROHVDOLYDVDPSOHVIURPWKHFKURQLFSHULRGRQWLWLV（CP）SDWLHQWVMaterials and Methods: Twenty CP patients were recruited and determined periodontal status by probing pocket depth（PD）,
bleeding on probing（BOP）DQGLQWUDRUDOUDGLRJUDSK\DQGZKROHVDOLYDZHUHFROOHFWHGDW¿UVWYLVLWDQGDIWHU,376DOLYDVDPSOHVZHUHVXEMHFWHGWRDUHDO‑WLPH3&5WRGHWHFWP. gingivalisDQG(%9'1$
Results: P. gingivalisDQG(%9'1$ZHUHGHWHFWHGLQ（100％）and 14（70％）saliva samples from the CP patients at¿UVWYLVLW$IWHU,37QXPEHURIGHWHFWLRQVRIP. gingivalisDQG(%9'1$ZHUH decreased to 17（85％）and 9（45％）
VDOLYDVDPSOHVIURPWKHSDWLHQWV&RH[LVWHQFHRIP. gingivalisDQG(%9'1$ZHUHGHWHFWHGLQVDOLYDVDPSOHVIURPWKH
SDWLHQWVDW¿UVWYLVLWDQGVLJQL¿FDQWO\GHFUHDVHGWR（40％）DIWHU,376LJQL¿FDQWLPSURYHPHQWVLQPHDQ3'DQG%23
ZHUHREVHUYHGDIWHU,37
Conclusion: 7KHVHUHVXOWVVXJJHVWWKDWWKH,37ZDVH൵HFWLYHLQLPSURYHPHQWRIFOLQLFDOSDUDPHWHUVVXFKDV3'DQG%23DQG
UHGXFLQJWKHFRH[LVWHQFHRIP. gingivalisDQG(%9LQWKHVDOLYDIURP&3SDWLHQWVJSP/JACP Poster Session AAP2020

4

GB‑07

%RQHUHJHQHUDWLRQE\QRYHOFRPELQDWLRQPDWHULDOVXVLQJSKRVSKRU\ODWHGSXOOXODQDQG
WULSKRVSKDWHFDOFLXP
Yasuhito Morimoto＊1,2, Tomoka Hasegawa2, Hiromi Hongo2,
Tsutomu Sugaya1, Yasuhiro Yoshida31RULR$PL]XND2
1Periodontology and Endodontology, 2Developmental Biology of Hard tissue,
Biomaterial Engineering, Faculty of Dental Medicine, Hokkaido University

3

Background and objective: Phosphorylated pullulan（PPL）LVDXQLTXHPDWHULDOZKLFKVKRZVDKLJKD൶QLW\WRFU\VWDOOLQH
FDOFLXPSKRVSKDWH,QWKLVVWXG\ZHKDYHH[DPLQHGDQRYHOFRPSRQHQWPL[HGZLWK33/DQGĮ‑tricalcium phosphate（TCP）
or ȕ‑7&3LILWKDVWKHSUHIHUDEOHRVWHRFRQGXFWLYLW\ZKHQJUDIWHGLQWRWKHERQHGHIHFWVMaterials and Methods: Bone defects with a diameter of 2 mm were formed in the tibial cortical bone of ten‑weeks‑old:LVWDU UDWV DQG WKHQ ILOOHG ZLWK 33/ ȕ‑TCP, Į‑TCP, PPL+ȕ‑TCP, or PPL+Į‑7&3$IWHU ‑, 2‑, and 4‑weeks, theUHJHQHUDWHGERQHVZHUHKLVWRFKHPLFDOO\HYDOXDWHGE\H[DPLQLQJKLVWRFKHPLVWU\RI75$3$/3RVWHRSRQWLQYRQ.RVVD
staining and element mapping using an electron probe micro‑analyzer（EPMA）

Results: 7KHUHZDVQRLQ¿OWUDWLRQRILQÀDPPDWRU\FHOOVLQWKHUHJLRQRIERQHGHIHFWVRIDOOWKHJURXSV$W‑ or 2‑weeks,
75$3‑reactive/cathepsin K‑positive osteoclasts and ALP‑positive osteoblasts were localized on the Į‑TCP/ȕ‑TCP
granules, as well as PPL, the surface of which showed osteopontin‑SRVLWLYLW\$WZHHNVQHZERQHVZHUHIRUPHGRQQRW
only the grafted Į‑TCP/ȕ‑7&3JUDQXOHVEXWDOVRGLUHFWO\RQWKH33/,QWHUHVWLQJO\WKHJUDIWHG33/DOORZHGWKHLQYDVLRQRI
vascular endothelial cells and osteoblastic cells inside, and also showed the distribution of calcium phosphate crystals when
HVWLPDWHGE\(30$DQGYRQ.RVVDVWDLQLQJ7KXV33/DSSHDUHGWRWUDSFDOFLXPSKRVSKDWHVLQVLGH
Conclusion: ,WVHHPVOLNHO\WKDW33/FRXOGHIIHFWLYHO\VHUYHDVDQDGHTXDWHVFDIIROGIRUERQHUHJHQHUDWLRQDQGWKDWWKHFRPELQHGFRPSRQHQWVZLWK33/DQG7&3FRXOGSURYLGHDIDYRUDEOHPLFURHQYLURQPHQWIRUERQHUHJHQHUDWLRQGB‑08/DPLQ$LVUHTXLUHGIRUPHFKDQLFDOVWUHVV‑GHSHQGHQWRVWHREODVWUHVSRQVHV$\DQR1DNDPXUD＊17DNDVKL1DNDPXUD2, Toshifumi Azuma2,3, Atsushi Saito1,3
1

Department of Periodontology, Tokyo Dental College, Tokyo, Japan, 2Department of Biochemistry, Tokyo Dental
College, Tokyo, Japan, 3Oral Health Science Center, Tokyo Dental College

Background and objective: /DPLQ$LVDQLQWHUPHGLDWH¿ODPHQWXQGHUO\LQJLQQHUQXFOHDUPHPEUDQHEHLQJFRQQHFWHGWRDFWLQF\WRVNHOHWRQYLD/,1&SURWHLQV,WFRQWUROVFKURPDWLQRUJDQL]DWLRQ'1$GXSOLFDWLRQDQGWUDQVFULSWLRQ:HK\SRWKHVL]HG
that cell nuclear membrane associated proteins like Lamin A play an important role to transmit mechanical stress to
RVWHRJHQHVLV PHFKDQLVP DQG WKXV DUH LQYROYHG LQ WKH SURJUHVVLRQ RI SHULRGRQWDO GLVHDVHV ,Q WKLV VWXG\ ZH DLP WR
LQYHVWLJDWHWKHUROHRI/DPLQ$LQWKHPHFKDQLFDOVWUHVVUHVSRQVHGXULQJRVWHREODVWLFGL൵HUHQWLDWLRQ
Materials and Methods: Lmna+/‑PLFHZHUHSXUFKDVHGIURPWKH-DFNVRQ/DERUDWRU\&DOYDULDO‑osteoblasts were isolated of
QHZERUQ:7DQGLmna‑/‑OLWWHUPDWHPLFH7KHFDOYDULDHZHUHGLJHVWHGIRUPLQXWHVDW℃ with Į0(0FRQWDLQLQJ％FROODJHQDVHDQG％GLVSDVH3ULPDU\RVWHREODVWVZHUHVHHGHGRQWRDVWUHWFKFKDPEHUDWDGHQVLW\RI[5 cells / well andFXOWXUHGLQRVWHREODVWGL൵HUHQWLDWLRQPHGLXPXQGHUFRQWLQXRXVF\FOLFVWUHWFKRI％ amplitude at a rate of 5 cycles/min forGD\VXVLQJ&HOO6WUHWFKLQJ6\VWHP2VWHREODVWLFGL൵HUHQWLDWLRQZDVDVVHVVHGE\$/3VWDLQLQJDQGTXDQWLWDWLYH57‑3&5IRU
Alpl, Col1a1, Runx2, and Bglap2
Results: ALP staining tended to be lower in Lmna‑/‑RVWHREODVWVWKDQ:7RVWHREODVWVDQGERWKGHFUHDVHGXQGHUPHFKDQLFDOVWUHVV7KH H[SUHVVLRQ OHYHOV RI PDUNHU JHQHV WHQGHG WR EH ORZHU LQ VWUHVVHG /PQD‑/‑ RVWHREODVWV WKDQ LQ VWUHVVHG:7
RVWHREODVWV(DUO\GLIIHUHQWLDWLRQPDUNHUVAlp, Col1a1, and Runx2 were decreased in a stress‑dependent manner in bothFHOOV
Conclusion: ,WLVVXJJHVWHGWKDW/DPLQ$LVLQYROYHGLQWKHPHFKDQLFDOVWUHVVUHVSRQVHGXULQJRVWHREODVWLFGL൵HUHQWLDWLRQ

JSP/JACP Poster Session AAP2020

5

GB‑09

$PHORJHQLQVXSSUHVVHV,)1Ȗ‑LQGXFHG0+&FODVV,,H[SUHVVLRQLQPDFURSKDJHVKaren Yotsumoto＊7HUXND]X6DQXL8UDUD7DQDND+LURDNL<DPDWR<XNL1DNDR<XNDUL:DWDQDEH&KLNDNR+D\DVKL7DNDR)XNXGD)XVDQRUL1LVKLPXUD'HSDUWPHQWRI3HULRGRQWRORJ\'LYLVLRQRI2UDO5HKDELOLWDWLRQ)DFXOW\RI'HQWDO6FLHQFH.\XVKX8QLYHUVLW\Background and objective: (QDPHOPDWUL[GHULYDWLYHV（EMDs）‑based regenerative therapy is known to promote better
KHDOLQJ ZLWK PLQLPDO LQIODPPDWRU\ UHVSRQVH DIWHU SHULRGRQWDO VXUJHU\:H SUHYLRXVO\ GHPRQVWUDWHG WKDW DPHORJHQLQVXSSUHVVHGPDMRUKLVWRFRPSDWLELOLW\FRPSOH[FODVV,,（0+&,,）JHQHH[SUHVVLRQLQPRQRF\WHVXVLQJ'1$PLFURDUUD\DQDO\VLV7KHDLPVRIWKLVVWXG\ZHUHWRXQGHUVWDQGWKHPROHFXODUPHFKDQLVPKRZDPHORJHQLQVXSSUHVVHG,)1Ȗ‑induced
0+&,,H[SUHVVLRQMaterials and Methods: THP‑PDFURSKDJHVZHUHSUHWUHDWHGZLWKDPHORJHQLQDQGVWLPXODWHGZLWK,)1Ȗ7KHFHOOVXUIDFH
H[SUHVVLRQRI0+&,,PROHFXOHVZDVHYDOXDWHGE\ÀRZF\WRPHWU\DQGVLJQDOWUDQVGXFWLRQZDVDQDO\]HGE\UHDO‑WLPH3&5
DQGZHVWHUQEORWWLQJ7KHXSWDNHRIODEHOHGDPHORJHQLQLQWRPDFURSKDJHVZDVPRQLWRUHGE\FRQIRFDOPLFURVFRS\+LVWRQH
PRGL¿FDWLRQRIFODVV,,WUDQVDFWLYDWRU（&,,7$）SURPRWHUUHJLRQZDVDVVHVVHGE\FKURPDWLQLPPXQRSUHFLSLWDWLRQ7FHOODFWLYDWLRQZDVHYDOXDWHGE\PL[HGOHXNRF\WHUHDFWLRQ（0/5）
Results: Amelogenin down‑PRGXODWHG,)1Ȗ‑LQGXFHGFHOOVXUIDFHH[SUHVVLRQRI0+&,,IROORZLQJDFFXPXODWLRQLQWKH
QXFOHXVDWPLQDQGLQKLELWLRQRIVXEVHTXHQW&,,7$H[SUHVVLRQ5HGXFHG0+&,,H[SUHVVLRQRQPDFURSKDJHVSUHWUHDWHG
with amelogenin down‑UHJXODWHG7FHOODFWLYDWLRQPDUNHUV7FHOOSUROLIHUDWLRQDQG,/‑SURGXFWLRQLQ0/5+.DFDQG
+.PH LQ &,,7$ SURPRWHU ,9 UHJLRQ ERWK RI ZKLFK DUH HVVHQWLDO IRU FRQYHUVLRQ WR HXFKURPDWLQ ZHUH PDUNHGO\VXSSUHVVHGE\DPHORJHQLQ
Conclusion: 7KHUHVXOWVVKRZWKDWDPHORJHQLQVXSSUHVVHV0+&,,H[SUHVVLRQE\DOWHULQJFKURPDWLQVWUXFWXUHDQGLQKLELWLQJ
&,,7$WUDQVFULSWLRQDFWLYLW\DQGDWWHQXDWHVVXEVHTXHQW7FHOODFWLYDWLRQ&OLQLFDOO\REVHUYHGDFFHOHUDWHGKHDOLQJDIWHUSHULRGRQWDOVXUJHU\E\(0'VPD\DWOHDVWLQSDUWEHPHGLDWHGE\WKHPHFKDQLVPHOXFLGDWHGLQWKLVVWXG\GB‑10

7KHUDSHXWLFSRWHQWLDORIH[RVRPHVGHULYHGIURP*06&LQSHULRGRQWDOGLVHDVH<XNL1DNDR＊7DNDR)XNXGD7DNDQRUL6KLQMR<XNDUL:DWDQDEH&KLNDNR+D\DVKL+LURDNL<DPDWR.DUHQ<RWVXPRWR8UDUD7DQDND7HUXND]X6DQXL)XVDQRUL1LVKLPXUD'HSDUWPHQWRI3HULRGRQWRORJ\'LYLVLRQRI2UDO5HKDELOLWDWLRQ)DFXOW\RI'HQWDO6FLHQFH.\XVKX8QLYHUVLW\Background and objective: Mesenchymal stem cells from gingiva（GMSCs）are easier to isolate, and appear to secrete
KLJKHUDPRXQWVRIH[RVRPHWKDQRWKHU06&V2XUSUHOLPLQDU\GDWDGHPRQVWUDWHGWKDWH[RVRPHIURP71)‑T‑stimulated
GMSCs markedly drove macrophage polarization toward anti‑LQÀDPPDWRU\（M2）phenotype, suggesting that this negative
IHHGEDFNORRSFRXOGEHEHQH¿FLDOIRUEHWWHUZRXQGKHDOLQJ7RIXUWKHULQYHVWLJDWHWKHWKHUDSHXWLFSRWHQWLDORIH[RVRPHVIURP
*06&VZHLQYHVWLJDWHGLWVH൵HFWRQDOYHRODUERQHORVVIURPWKHYLHZSRLQWRIH[RVRPDOPL51$
Materials and Methods: *06&VZHUHLVRODWHGIURPJLQJLYDOWLVVXHVXQGHUWKHDSSURYDORI,QVWLWXWLRQDO%RDUGSURWRFRO（2019‑374）DQGWKHH[RVRPHVZHUHSXUL¿HGIURPFXOWXUHVXSHUQDWDQW7RH[DPLQHWKHSURWHFWLYHUROHRI*06&‑derived
H[RVRPHVRQSHULRGRQWDOWLVVXHGHVWUXFWLRQWKHOLJDWXUH‑LQGXFHGDOYHRODUERQHORVVZDVFUHDWHGLQPLFH([RVRPHVZHUH
LQMHFWHGLQWRWKHSDODWDOJLQJLYDRIPLFHXVLQJDVSOLW‑PRXVHH[SHULPHQWDOGHVLJQ7KHH൵HFWRIH[RVRPHIURP*06&VRQ
5$1./ H[SUHVVLRQ LQ /36‑stimulated human periodontal ligament cells（hPDLCs）ZDV H[DPLQHG$IWHU71)‑VWLPXODWLRQH[SUHVVLRQSUR¿OHVRIH[RVRPDOPL51$LQ*06&VZHUHDQDO\]HGE\PL51$DUUD\6LJQDOLQJSDWKZD\VOHDGLQJ
to LPS‑LQGXFHG5$1./H[SUHVVLRQZHUHDQDO\]HGDQGWKHLQYROYHPHQWRIH[RVRPDOPL51$LQ5$1./H[SUHVVLRQZDVLQYHVWLJDWHG
Results: ([RVRPHVIURP*06&VPDUNHGO\VXSSUHVVHGDOYHRODUERQHUHVRUSWLRQLQPLFHPRGHORISHULRGRQWLWLV([RVRPH
IURP*06&VLJQL¿FDQWO\LQKLELWHG5$1./H[SUHVVLRQLQ/36‑WUHDWHG3'/&VDQGWKHH൵HFWZDVIXUWKHUHQKDQFHGE\SULRUWUHDWPHQWRI*06&VZLWK71)‑
Conclusion: ([RVRPHVGHULYHGIURP*06&VVXSSUHVVHGERQHORVVDQG71)‑ ‑LQGXFHGH[RVRPDOPL51$ZDVHVVHQWLDOIRU
WKHVXSSUHVVLRQRI5$1./H[SUHVVLRQJSP/JACP Poster Session AAP2020

6

GB‑11

&RPELQHGH൵HFWVRIJHUDQ\OJHUDQ\ODFHWRQHDQGDPHORJHQLQRQSHULRGRQWDOWLVVXHUHJHQHUDWLRQ
Hiroaki Yamato＊7HUXND]X6DQXL.DUHQ<RWVXPRWR<XNL1DNDR<XNDUL:DWDQDEH&KLNDNR+D\DVKL7DNDR)XNXGD8UDUD7DQDND)XVDQRUL1LVKLPXUD'HSDUWPHQWRI3HULRGRQWRORJ\'LYLVLRQRI2UDO5HKDELOLWDWLRQ)DFXOW\RI'HQWDO6FLHQFH.\XVKX8QLYHUVLW\Background and objective: :HSUHYLRXVO\UHSRUWHGWKDWDPHORJHQLQ（rM180）directly binds to glucose‑regulated protein
78（*53）, a member of heat‑VKRFN SURWHLQ IDPLO\ DQG WKDW FHOO PLJUDWLRQ DFWLYLW\ ZDV HQKDQFHG ZKHQ *53‑RYHUH[SUHVVLQJKXPDQSHULRGRQWDOOLJDPHQWFHOOV（hPDLCs）ZHUHWUHDWHGZLWKU0*HUDQ\OJHUDQ\ODFHWRQH（GGA）isa heat‑shock protein‑LQGXFLQJ PHGLFDWLRQ WKDW LV FRPPRQO\ XVHG WR WUHDW JDVWULF XOFHUV ,Q WKLV VWXG\ WKHUHIRUH ZH
K\SRWKHVL]HGWKDW**$DQGDPHORJHQLQH[KLELWV\QHUJLVWLFH൵HFWIRUEHWWHUKHDOLQJDQGWHVWHGLWVSRVVLELOLW\XVLQJK3'/&V
Materials and Methods:
**$‑LQGXFHG*53H[SUHVVLRQDQGLWVH൵HFWRQFHOOIXQFWLRQVZDVHYDOXDWHG
0LFURDUUD\DQDO\VHVWRLGHQWLI\JHQHVXSUHJXODWHGE\WKHVWLPXODWLRQZLWKRUZLWKRXW**$RU**$U0LQK3'/&V
DQGLWVYHUL¿FDWLRQZDVSHUIRUPHG
7KHWXEHIRUPDWLRQE\VXSHUQDWDQWVIURPK3'/&VWUHDWHGZLWKHDFKVWLPXODWLRQZDVLQYHVWLJDWHGXVLQJKXPDQXPELOLFDO
vein endothelial cells（HUVECs）

Results & Conclusion: :HIRXQGWKDW**$LQFUHDVHG*53SURWHLQH[SUHVVLRQDWKRXUVDIWHUWKHVWLPXODWLRQDQG
HQKDQFHGFHOOPLJUDWLRQ0LFURDUUD\DQDO\VLVGHPRQVWUDWHGWKDWWKHLQFUHDVHG*53H[SUHVVLRQWULJJHUHGWKHSURGXFWLRQRIDQJSWODQGDPSKLUHJXOLQERWKRIZKLFKDUHLQYROYHGLQHQKDQFHGDQJLRJHQHVLVDQGVXEVHTXHQWZRXQGKHDOLQJ0RUHRYHU
the addition of rM180 further accelerated the hPDLCs cell migration and tube formation of HUVECs due to the upregulation
RI,/‑8, MCP‑DQG,/‑6, all of which are known as angiogenesis‑LQGXFLQJIDFWRUV1HXWUDOL]LQJDQWLERGLHVDJDLQVWWKHVH
F\WRNLQHVVLJQLILFDQWO\LQKLELWHGWXEHIRUPDWLRQE\+89(&V7KXVWKHFRPELQHGDSSOLFDWLRQRI**$DQGU0PD\FUHDWHDVXLWDEOHHQYLURQPHQWIRUSHULRGRQWDOZRXQGKHDOLQJGB‑12,QYHVWLJDWLQJWKHPHFKDQLVPRIHQYLURQPHQWDOVWUHVVUHVSRQVHLQ Treponema denticola
Toshiya Fukasawa＊1,2, Yurie Kitamura1, Keiko Yamashita1, Hiroyuki Asai3.D]X\XNL,VKLKDUD2,4,Atsushi Saito1,2
1

Department of Periodontology, Tokyo Dental College, 2Oral Health Science Center, Tokyo Dental College,
3
Asai Dental Clinic, 4Department of Microbiology, Tokyo Dental College

Background and objective: %DFWHULDDUHH[SRVHGWRHQYLURQPHQWDOVWUHVVHVLQRUDOFDYLW\7RDGDSWWRHQYLURQPHQWDOVWUHVVHVEDFWHULDXVXDOO\UHJXODWHJHQHH[SUHVVLRQVXVLQJ'1$‑ELQGLQJSURWHLQVTreponema denticola has been frequently isolated
IURPWKHOHVLRQRIFKURQLFSHULRGRQWLWLVDQGPXVWRYHUFRPHHQYLURQPHQWDOVWUHVVHVWRFRORQL]H:HKDYHUHSRUWHGWKDWWKH
H[SUHVVLRQVRIVRPHJHQHVFRGLQJIRU'1$‑binding protein in T. denticola are regulated by environmental stress such as
ORZQXWULHQWFRQGLWLRQV7KHSXUSRVHRIWKLVVWXG\LVWRLQYHVWLJDWHWKHUROHRIDQRYHOT. denticola'1$‑binding protein in
the response to environmental stresses.
Materials and Methods: $PXWDQWGH¿FLHQWLQWKHJHQHFRGLQJIRUD'1$‑binding protein was constructed by homologous
UHFRPELQDWLRQ8QGHUORZQXWULHQWFRQGLWLRQVWKHJURZWKDQGSURWHRO\WLFDFWLYLWLHV（dentilisin and oligopeptidase B）were
HYDOXDWHG7KHYLDELOLW\RIWKHFHOOVZDVPHDVXUHGXQGHUR[\JHQRVPRWLFGRZQVKLIWDQGKHDWVWUHVVHVResults: 8QGHUORZQXWULHQWPHGLXPWKHJURZWKRIWKHPXWDQWVWUDLQZDVVLJQL¿FDQWO\GHFUHDVHGFRPSDUHGZLWKZLOGW\SHDW
24‑KRXUVDQGWKHGHQWLOLVLQDFWLYLW\RIWKHPXWDQWVWUDLQZDVVLJQLILFDQWO\GHFUHDVHGFRPSDUHGZLWKZLOGW\SH7KH
YLDELOLW\RIWKHPXWDQWVWUDLQZDVVLJQL¿FDQWO\GHFUHDVHGXQGHUR[\JHQVWUHVVDQGLQFUHDVHGXQGHUKHDWVWUHVVDWKRXUV1RVLJQL¿FDQWGL൵HUHQFHZDVREVHUYHGEHWZHHQWKHPXWDQWDQGZLOGW\SHVWUDLQVXQGHURVPRWLFGRZQVKLIWVWUHVVConclusion: ,WLVVXJJHVWHGWKDWWKH'1$‑binding protein is involved in the growth, dentilisin activity and survival of T.
denticolaXQGHUHQYLURQPHQWDOVWUHVVHVVXFKDVORZQXWULHQWR[\JHQDQGKHDWJSP/JACP Poster Session AAP2020

7

GB‑13

5ROHVRI3'/,0LQSHULRGRQWDOOLJDPHQWDQGJLQJLYDOHSLWKHOLDOFHOOVAsuka Yuda＊16KLQVXNH)XMLL2&$0F&XOORFK3, Michihiko Usui4, Shinya Murakami5,
Hidefumi Maeda61DRKLVD:DGD1
1

Division of General Dentistry, Kyushu University Hospital, Kyushu University, 2Laboratory of Oral Pathology,'LYLVLRQRI0D[LOORIDFLDO'LDJQRVWLFDQG6XUJLFDO6FLHQFHV)DFXOW\RI'HQWDO6FLHQFH.\XVKX8QLYHUVLW\3Facultyof Dentistry, University of Toronto, 4Division of Periodontology, Department of Oral Function, Kyushu Dental
University, 5Department of Periodontology, Osaka University Graduate School of Dentistry, 6Division of Oral
5HKDELOLWDWLRQ'HSDUWPHQWRI(QGRGRQWRORJ\DQG2SHUDWLYH'HQWLVWU\Background and objective: During wound hearing, inhibition of gingival downgrowth play a critical role in leading to
VXFFHVVIXOSHULRGRQWDOUHJHQHUDWLRQ&HOOH[WHQVLRQVSOD\DQLPSRUWDQWUROHLQVHYHUDOSURFHVVHVVXFKDVZRXQGKHDOLQJDQG
PDWUL[UHPRGHOLQJ3'/,0LVDPHPEHURIWKH3'=‑/,0SURWHLQV:HUHFHQWO\UHSRUWHGWKDW3'/,0ZDVUHTXLUHGIRUFHOOH[WHQVLRQVIRUPDWLRQLQPRXVH¿EUREODVWFHOOV,QWKLVVWXG\ZHLQYHVWLJDWHGWKHH൵HFWVRI3'/,0RQDVVRFLDWLRQZLWKFHOOH[WHQVLRQVIRUPDWLRQDQGPLJUDWLRQDFWLYLW\RIKXPDQSHULRGRQWDOOLJDPHQW（HPDL）and gingival epithelial（HGE）FHOOV
Materials and Methods: :HXVHGW\SHVRIFHOOV；SV40T‑$JDQGKXPDQ7(57LPPRUWDOL]HG+3'/¿EUREODVWFHOOOLQHV（1‑11）,
and SV40T‑Ag immortalized HGE cell lines（OBA‑9）
3'/,0H[SUHVVLRQLQ‑11 and OBA‑ZDVFRQ¿UPHGE\LPPXQREORWDQDO\VLV7RH[DPLQHWKHUROHVRI3'/,0LQ‑DQG2%$3'/,0ZDVNQRFNHGGRZQE\WZRGL൵HUHQWVL51$V‑11 andOBA‑ZLWK3'/,0‑knockdown were planted on grid‑VXSSRUWHGÀRDWLQJFROODJHQJHOVDQGIRUPDWLRQRIFHOOH[WHQVLRQVZDVH[DPLQHG‑11 and OBA‑ZLWK3'/,0‑NQRFNGRZQZHUHFXOWXUHGLQFHOOFXOWXUHLQVHUWVIRUFHOOPLJUDWLRQDVVD\Results: 3'/,0H[SUHVVLRQZDVREVHUYHGE\LPPXQREORWDQDO\VLVLQ‑11 and OBA‑‑ZLWK3'/,0‑knockdown
UHGXFHGFHOOH[WHQVLRQVIRUPDWLRQDQGPLJUDWLRQDFWLYLWLHVFRPSDUHGZLWKFRQWUROFHOOVOBA‑ZLWK3'/,0‑NQRFNGRZQSURPRWHGFHOOH[WHQVLRQVIRUPDWLRQDQGPLJUDWLRQDFWLYLWLHVFRPSDUHGZLWKFRQWUROFHOOV
Conclusion: 7KHVHUHVXOWVVXJJHVWHGWKDW3'/,0PD\UHJXODWHFHOOH[WHQVLRQVIRUPDWLRQDQGPLJUDWLRQDFWLYLW\RI+3'/
DQG+*(FHOOVGXULQJZRXQGKHDULQJ3'/,0ZRXOGEHXVHGDVDWKHUDSHXWLFDJHQWIRUSHULRGRQWDOUHJHQHUDWLRQGB‑14)XQFWLRQDODQDO\VLVRID'1$ELQGLQJSURWHLQ‑FRGLQJJHQHLQTreponema denticola
Keiko Yamashita＊1, Yurie Kitamura1,2, Toshiya Fukasawa1,2, Atsushi Saito1,2.D]X\XNL,VKLKDUD2,3
1

Department of periodontology, Tokyo Dental College, 2Oral health science center, Tokyo Dental College,3
Department of microbiology, Tokyo Dental College

Background and objective: Treponema denticolaLVRQHRIWKHSUHGRPLQDQWEDFWHULDLQGHHSSHULRGRQWDOSRFNHWVT. denticola
KDVYDULRXVYLUXOHQFHIDFWRUVVXFKDVPDMRURXWHUVKHDWKSURWHLQ（Msp）+RZHYHUWKHPHFKDQLVPRIWKH0VSH[SUHVVLRQKDV\HWWREHFODUL¿HG:HSUHYLRXVO\IRXQGVRPHJHQHVFRGLQJIRUWUDQVFULSWLRQDOUHJXODWRUVZKLFKPLJKWEHLPSOLFDWHGLQthe regulation of mspH[SUHVVLRQ$PRQJWKHPZHIRFXVHGRQDJHQHFRGLQJIRUD'1$ELQGLQJSURWHLQ7KHREMHFWLYHRIWKLVVWXG\LVWRFODULI\WKHUHJXODWLRQPHFKDQLVPRI0VSH[SUHVVLRQWKURXJKDQDO\VLVRIWKHIXQFWLRQRIWKHJHQHHQFRGLQJWKH
'1$ELQGLQJSURWHLQ
Materials and Methods: :HFRQVWUXFWHGWKHPXWDQWVWUDLQGHILFLHQWLQWKHJHQHFRGLQJIRUWKH'1$ELQGLQJSURWHLQE\
KRPRORJRXVUHFRPELQDWLRQ8VLQJWKLVQHZO\FRQVWUXFWHGPXWDQWZHSHUIRUPHGJHQHWLFDQDO\VLVE\'1$PLFURDUUD\DQG
T57‑3&5%DVHGRQWKHUHVXOWVRIWKHJHQHWLFDQDO\VLVZHDOVRDQDO\]HGVRPHSKHQRW\SLFFKDQJHVRIWKHPXWDQW
Results: 7KHH[SUHVVLRQOHYHOVRImsp or other genes coding for virulence factors in the mutant were similar to those of wild
W\SH+RZHYHUWKHJHQHVLPSOLFDWHGLQWKHPRWLOLW\RIT. denticolaZHUHVLJQL¿FDQWO\XSUHJXODWHG$OVRWKHPRYLQJSDWWHUQRIWKHPXWDQWZDVFRQVLGHUDEO\FKDQJHG
Conclusion: 7KHJHQHFRGLQJIRUWKH'1$ELQGLQJSURWHLQGRHVQRWSOD\DGLUHFWUROHLQWKHUHJXODWLRQRImsp or other genes
FRGLQJIRUYLUXOHQFHIDFWRUV+RZHYHULWLVVXJJHVWHGWKDWWKH'1$ELQGLQJSURWHLQSOD\VDFHUWDLQUROHLQWKHUHJXODWLRQRI
the genes implicated in the motility of T. denticola

JSP/JACP Poster Session AAP2020

8

GB‑15

%XW\ULFDFLGPRGXODWHVSHULRGRQWDOPHFKDQLFDOVHQVLWLYLW\LQSHULRGRQWLWLVLQPLFH
1DRNL0XUDNDPL＊1, 2, Masamichi Shinoda2.RLFKL,ZDWD2+LGHND]X1DJDVKLPD1,
Hirotaka Akiyama1, Shingi Arai1, Shuichi Sato1
1'HSDUWPHQWRI3HULRGRQWRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\2'HSDUWPHQWRI3K\VLRORJ\1LKRQ
University School of Dentistry

Background and objective: ,WLVZHOONQRZQWKDWSHULRGRQWLWLVJUDGXDOO\SURJUHVVHVDFFRPSDQLHGE\DOYHRODUERQHORVVDQG
SURJUHVVLYH LQIODPPDWLRQ ZLWKRXW SHULRGRQWDO SDLQ +RZHYHU WKH PRGXODWLQJ PHFKDQLVP RI SHULRGRQWDO PHFKDQLFDOVHQVLWLYLW\LQSHULRGRQWLWLVLVSRRUO\XQGHUVWRRG,QWKLVVWXG\ZHH[DPLQHGWKHLQYROYHPHQWRIEXW\ULFDFLGLQWKHFKDQJHVLQSHULRGRQWDOPHFKDQLFDOVHQVLWLYLW\LQH[SHULPHQWDOSHULRGRQWLWLVSURGXFHGE\Porphyromonas gingivalis（P.g.）LQPLFH
Materials and Methods: Under deep anesthesia, a 5‑VLONOLJDWXUHZDVWLHGDURXQGWKHPD[LOODU\VHFRQGPRODUDQGP.g. was
LQRFXODWHGLQWRWKHWLHGVLONOLJDWXUHWRHVWDEOLVKWKHPXULQHPRGHORIH[SHULPHQWDOSHULRGRQWLWLV8QGHUOLJKWDQHVWKHVLDPHFKDQLFDOVWLPXODWLRQZDVDSSOLHGWRWKHJLQJLYDOWLVVXHDGMDFHQWWRFHUYLFDOUHJLRQVRIWKHPD[LOODU\VHFRQGPRODUE\XVLQJelectronic von Frey anesthesiometer and the mechanical head withdrawal threshold（0+:7）ZDVPHDVXUHGIRUGD\V
2QGD\IROORZLQJWKHOLJDWLRQWKHH[SUHVVLRQRIVKRUW‑FKDLQIDWW\DFLGUHFHSWRU*35LQWULJHPLQDOJDQJOLRQDQGWKH
DPRXQW RI EXW\ULF DFLG LQ WKH LQIODPHG SHULRGRQWDO WLVVXH 0RUHRYHU ZH H[DPLQHG WKH FKDQJHV LQ WKH 0+:7 LQ WKHH[SHULPHQWDOSHULRGRQWLWLVPRGHOE\GDLO\LQWUD‑SHULRGRQWDO*35DQWDJRQLVWK\GUR[\EXW\ULFDFLGDGPLQLVWUDWLRQResults: 7KHUHZDVQRFKDQJHLQWKH0+:7LQWKHH[SHULPHQWDOSHULRGRQWLWLVPRGHOIRUGD\V*35ZDVH[SUHVVHGLQ
WULJHPLQDOJDQJOLRQQHXURQVDQGEXW\ULFDFLGZDVLQFUHDVHGRQGD\IROORZLQJWKHOLJDWLRQ7KHGDLO\K\GUR[\EXW\ULFDFLGDGPLQLVWUDWLRQGHFUHDVHGWKH0+:7GHFUHDVHGRQGD\IROORZLQJWKHOLJDWLRQ
Conclusion: Butyric acid produced by P.g. in the periodontal tissue may contribute to periodontal mechanical sensitivity in
SHULRGRQWLWLV

GB‑16,QYHVWLJDWLRQRIMXQFWLRQDOHSLWKHOLDOVWHPSURJHQLWRUFHOOVXVLQJWKHPXOWLFRORUOLQHDJHWUDFLQJPHWKRG
Keisuke Tanaka＊1, 2, Junichi Tanaka25\R$L]DZD1, Mayu Kato‑Tanaka1,
Hiroo Ueno3.HQML0LVKLPD2, Matsuo Yamamoto1
1

Department of Periodontology, Showa University School of Dentistry, 2Division of Pathology, Department of Oral
Diagnostic Sciences, Showa University School of Dentistry, 3Department of Stem Cell Pathology, Kansai Medical
University

Background and objective: 7KHMXQFWLRQDOHSLWKHOLXP（JE）has a distinctive contribution to infection prevention, owing to
LWVORFDWLRQRQWKHIURQWOLQHVRIGHIHQVHLQWKHRUDOFDYLW\ZKHUHLWGLUHFWO\FRQWDFWVWKHWRRWKVXUIDFH+RZHYHULWUHPDLQVXQFOHDUZKHWKHUWKHVWHPSURJHQLWRUFHOOVH[LVWLQWKH-(7KHDLPRIWKLVVWXG\ZDVWRLQYHVWLJDWHWKHFHOOOLQHDJHDQGWKH
H[LVWHQFHRIVWHPSURJHQLWRUFHOOVLQWKH-(XVLQJWKHPXOWLFRORUOLQHDJHWUDFLQJPHWKRG
Materials and Methods: All of the cells in the body were randomly labeled with four colors（green: GFP, blue: mCerulean,orange: mOrange, red: mCherry）XVLQJ5RVD&UH(57UEZPLFHDWSRVWQDWDOZHHNZLWKWDPR[LIHQ（TAM）7KHFHOOOLQHDJHZDVDQDO\]HGLQWKH-(RI5RVD&UH(57UEZPLFHDWGD\VDQGZHHNVDIWHU7$0LQMHFWLRQE\ÀXRUHVFHQFHPLFURVFRS\Results: /DEHOHGFHOOVZLWKIRXUFRORUVZHUHIRXQGLQWKH-(RI5RVD&UH(57UEZPLFHDWGD\VDIWHU7$0LQMHFWLRQ7ZR
UHJLRQVZLWKLQ-(ZHUHGLVWLQJXLVKHGE\FRORUVDQGZHUHGL൵HUHQWIURPWKHRUDOHSLWKHOLXPLQ5RVD&UH(57UEZ mice at 4 andZHHNVDIWHU7$0LQMHFWLRQ)XUWKHUPRUHWKUHH‑GLPHQVLRQDOLPDJLQJLQGLFDWHGWKHH[LVWHQFHRIWZRFORQDOFHOOVLQWKH-(RIWKRVHPLFH
Conclusion: 3UHVHQWVWXG\SRVVLEO\VXJJHVWHGWKHH[LVWHQFHRIVWHPSURJHQLWRUFHOOVLQWKH-(7KHORQJ‑term multicolor
lineage tracing observations will be needed to determine whether the stem/progenitor cells of JE survives in the long term
DQGFRQWULEXWHWRKRPHRVWDVLVRI-(JSP/JACP Poster Session AAP2020

9

GB‑17

(QGRWR[HPLDE\Porphyromonas gingivalisDJJUDYDWHVLQÀDPPDWLRQLQEURZQDGLSRVHWLVVXH
Masahiro Hatasa＊1<XMLQ2KVXJL1, Sayaka Katagiri1, Tomomitsu Hirota2.D]XNL:DWDQDEH1,
Shogo Maekawa11DRNL6DVDNL3+LURPL1LLPL1, Tuyoshi Shimohira1, Kazuki Morita1,
Sumiko Yoshida1+LURVKL1LWWD37DNDQRUL,ZDWD1
1Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University,
2'HSDUWPHQWRI0ROHFXODU*HQHWLFV5HVHDUFK&HQWHUIRU0HGLFDO6FLHQFH7KH-LNHL8QLYHUVLW\6FKRRORI0HGLFLQH
3
Behavioral Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

Background and objective: $GLSRVHWLVVXHLVNQRZQDVQRWRQO\VWRUDJHRUJDQIRUHQHUJ\EXWDOVRHQGRFULQHRUJDQ6LQFH
adipocytes produce various kinds of adipocytokines, including adiponectin and inflammatory cytokines, many studies
VXJJHVWHG WKH UHODWLRQVKLS EHWZHHQ DGLSRF\WHV DQG V\VWHPLF GLVHDVHV (VSHFLDOO\ VHYHUDO UHSRUWV KDYH VKRZQ WKDW WKHIXQFWLRQDO GHFOLQH RI EURZQ DGLSRVH WLVVXH LV UHODWHG WR REHVLW\ 3UHYLRXVO\ ZH UHSRUWHG WKDW LQWUDYHQRXV LQMHFWLRQ RI
sonicated Porphyromonas gingivalis（Pg）LQFUHDVHVERG\ZHLJKWDQGYLVFHUDOIDWYROXPHVLQPLFH+RZHYHUWKHGLUHFWH൵HFWVRIEDFLOODHPLDE\PgRQDGLSRF\WHVUHPDLQXQFOHDU7KLVVWXG\ZDVSHUIRUPHGWRHYDOXDWHWKHH൵HFWRIVRQLFDWHGPg
LQMHFWLRQRQDGLSRF\WHVLQVKRUW‑WHUP
Materials and Methods: (QGRWR[HPLDZDVLQGXFHGLQPDOH&%/-PLFH（12‑weeks‑old）E\LQWUDYHQRXVLQMHFWLRQRI
sonicated Pg7KHP51$H[SUHVVLRQOHYHOLQEURZQDGLSRVHWLVVXHIURPLQWHUVFDSXODUIDWZDVHYDOXDWHGE\T3&5DWKRXUV
after Pg LQMHFWLRQ0LFURDUUD\DQDO\VLVZDVSHUIRUPHGLQEURZQDGLSRVHWLVVXHDWKRXUVDIWHUPg LQMHFWLRQResults: 7KH P51$ H[SUHVVLRQ RI LQIODPPDWLRQ‑related genes in brown adipose tissue were increased, and Adipoq
H[SUHVVLRQZDVGHFUHDVHG*HQHVHWHQULFKPHQWDQDO\VLVUHYHDOHGHQULFKHGPHQWRIVHYHUDOLQÀDPPDWLRQ‑related gene sets
DQGGRZQUHJXODWLRQRIVHYHUDOJHQHVHWVLQFOXGLQJ3,.$.7P725VLJQDOLQJFKROHVWHUROKRPHRVWDVLVDQGELOHDFLGVmetabolism in brown adipose tissue at 18 hours after Pg LQMHFWLRQ
Conclusion: (QGRWR[HPLDE\SHULRGRQWLWLVPD\HOLFLWLQÀDPPDWLRQDQGFKDQJHWKHHQGRFULQHDQGOLSLGPHWDEROLVPLQEURZQDGLSRVHWLVVXH7KHVHUHVXOWVVXJJHVWHGWKDWHQGRWR[HPLDE\SHULRGRQWLWLVPLJKWD൵HFWREHVLW\WKURXJKGLVWXUEDQFHRIDGLSRF\WHV

GB‑18,QVXOLQUHVLVWDQFHLQJLQJLYDO¿EUREODVWVH[DFHUEDWHVH[SHULPHQWDOSHULRGRQWLWLVLQPLFH
7DNDQRUL6KLQMR＊1, Thomas Van Dyke2, Hatice Hasturk2, George L King3)XVDQRUL1LVKLPXUD1
1

Section of Periodontology, Kyushu University, 2)RUV\WK,QVWLWXWH3Joslin Diabetes Center, Harvard Medical School

Background and objective: 3HULRGRQWLWLVLVNQRZQWREHH[DFHUEDWHGDQGLQVXOLQUHVLVWDQFHLQWKHJLQJLYDZDVVKRZQLQLQ
REHVLW\DQGGLDEHWHV:HDLPHGGHWHUPLQHKRZDQGLQZKLFKW\SHRIFHOOLQVXOLQUHVLVWDQFHFRQWULEXWHVWRH[DFHUEDWLRQRISHULRGRQWLWLVLQREHVLW\DQGGLDEHWHV
Materials and Methods: Smooth muscle protein 22Į‑Cre‑,5fl/fl（60,5.2）PLFHZHUHJHQHUDWHGE\FURVVEUHHGLQJ,5fl/fl
mice with SM22Į‑&UHPLFH60,5.2ZLOG‑type（:7）DQG:7PLFHIHGZLWKKLJK‑fat‑diet（HFD）for 8 weeks were
XVHG*LQJLYDVDPSOHVZHUHVWLPXODWHGZLWK‑Q0LQVXOLQIRUPLQXWHV([SHULPHQWDOSHULRGRQWLWLVZDVLQGXFHGE\
7‑VLONOLJDWLRQDURXQGPD[LOODU\QGPRODUVIRUDQGGD\V$GGLWLRQDOO\SULPDU\JLQJLYDO¿EUREODVWV（GFs）were
stimulated with E.coli lipopolysaccharides（LPS）for 6 hours in the presence or absence of pretreatment of 100nM insulin
SUHWUHDWPHQWIRUPLQXWHVResults: Gingival insulin receptor‑beta（,5ȕ）H[SUHVVLRQLQ60,5.2DQG+)'‑fed mice was decreased by 70％ and 50％, respectively, with comparable reductions in insulin‑LQGXFHGSKRVSKRU\ODWLRQRI$NW/LJDWXUH‑induced alveolar bone
ORVVLQ60,5.2DQG+)'‑fed mice was greater by 70％ and 40％ , respectively, as compared to regular diet（5'）‑fed:7PLFH1HXWURSKLOUHFUXLWPHQWZDVGHOD\HGVLJQL¿FDQWO\LQERWK60,5.2DQG+)'‑IHGPLFH6LPLODUO\GHFUHDVHGJHQH
H[SUHVVLRQRIWKHQHXWURSKLO‑UHFUXLWLQJIDFWRU&;&/ZDVVHHQLQWKHJLQJLYDDWGD\8QOLNH60,5.2DQG+)'PRXVH
*)V:7*)VVKRZHGWKDWLQVXOLQHQKDQFHG/36‑LQGXFHG&;&/H[SUHVVLRQConclusion: 7KHVHGDWDSURYLGHGWKH¿UVWHYLGHQFHWKDWLQVXOLQDFWLRQLVFULWLFDOIRUQHXWURSKLOUHFUXLWPHQWGXULQJLQIHFWLRQE\HQDEOLQJ&;&/H[SUHVVLRQLQ*)VZKLFKLVLQKLELWHGLQGLDEHWHVLQUHVSRQVHWREDFWHULDO/36VWLPXODWLRQ

JSP/JACP Poster Session AAP2020

10

GB‑19

&RPELQDWLRQRIIXQFWLRQDOZDWHUZLWKK\GUR[\DSDWLWHFROODJHQFRPSRVLWHDFFHOHUDWHERQH
UHJHQHUDWLRQ
Yasumasa Ozawa＊17DNDKLUR*RMRXERUL2, Hirotaka Ota2, 3, Akira Hasuike1, 4, Hisashi Suguro4, 5,
7HWVX\D1LVKLGD1, 4, Osamu Kishida1, Yuichi Saito1, Masatake Asano2, 3, Shuichi Sato1, 4
1'HSDUWPHQWRI3HULRGRQWRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\ , 2'HSDUWPHQWRI3DWKRORJ\1LKRQ8QLYHUVLW\School of Dentistry, 3'LYLVLRQRI,PPXQRORJ\DQG3DWKRELRORJ\'HQWDO5HVHDUFK&HQWHU1LKRQ8QLYHUVLW\6FKRRO
of Dentistry, 4'LYLVLRQRI$GYDQFHG'HQWDO7UHDWPHQW'HQWDO5HVHDUFK&HQWHU1LKRQ8QLYHUVLW\6FKRRORI
Dentistry, 5'HSDUWPHQWRI(QGRGRQWLFV1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\

Background and objective: +\GUR[\DSDWLWH  FROODJHQ FRPSRVLWH（HA/Col）FDQ UHJHQHUDWLYH ERQH$FLG HOHFWURO\]HG
functional water（):）JHQHUDWHGE\HOHFWURO\]LQJVDOLQHKDVDVWHULOL]LQJH൵HFW:HKDYHUHSRUWHGWKDW):DFFHOHUDWHWKH
regeneration in rat mandibular boneʼVGHIHFW7KHUHIRUHZHHYDOXDWHGWKHH൵HFWVRIWKHVHFRPELQDWLRQV
Materials and Methods: Artificial bone defects（PPGLDPHWHU）were made on the 8‑week‑ROGIHPDOH:LVWHUUDWVFDOYDULD7KHFRPELQDWRULDOJURXSPLQXWH):LUULJDWLRQDQG+$&RO¿OOLQJ7KH+$&ROJURXSPLQXWH3%6LUULJDWLRQ
DQG+$&RO¿OOLQJ7KH):JURXSPLQXWH):LUULJDWLRQ+&/JURXSPLQXWH+&/LUULJDWLRQ7KHFRQWUROJURXS3%6
LULJDWLRQ1HZERQHZDVPHDVXUHGE\PLFUR‑&7IRUZHHNVDIWHUVXUJHU\3HULSKHUDOEORRGZDVFROOHFWHGEHIRUHDQG
DQGKDIWHUVXUJHU\DQGVXEMHFWHGWR(/,6$7KHVXUJLFDODUHDZDVREVHUYHGKLVWRORJLFDOO\DQGDQDO\]HGJHQHWLFDOO\Results: 1HZERQHZDVVLJQL¿FDQWO\HQKDQFHGLQWKHFRPELQDWLRQDOJURXSDIWHU‑ZHHNVRIVXUJHU\7KHHQKDQFHGERQH
IRUPDWLRQZDVFRQ¿UPHGZLWKKLVWRORJLFDOH[DPLQDWLRQ（HE）DQGWKH75$3VWDLQLQJUHYHDOHGWKHLQFUHDVHGDFFXPXODWLRQRIRVWHRFODVWVDURXQGWKHERQ\ULPLQWKHFRPELQDWLRQDOJURXS5HDO‑WLPH3&5DQDO\VLVUHYHDOHGWKDWWKHH[SUHVVLRQRI
osteogenesis‑ and angiogenesis‑UHODWHGP51$VZDVLQFUHDVHLQWKHFRPELQDWLRQDOJURXS$PRQJWKHP)*)FRQFHQWUDWLRQLQWKHSHULSKHUDOEORRGLQFUHDVHGLQWKHFRPELQDWLRQDOJURXSDQGKRXUVDIWHUVXUJHU\Conclusion: 7KHVH¿QGLQJVVXJJHVWWKDW):IDFLOLWDWHVQHZERQHIRUPDWLRQE\LQGXFLQJDQJLRJHQHVLV‑ and bone remodeling‑
UHODWHGJHQHV7DNHQWRJHWKHU):ZDVVKRZQWRLPSURYHWKHERQHUHJHQHUDWLRQZKHQXVHGLQFRPELQDWLRQZLWK+$&RO
GB‑20

5ROHRITXLHVFHQWRVWHRFODVWSUHFXUVRUVLQRFFOXVDOWUDXPDLQPLFH
Yasunori Yamashita＊, Yukio Ozaki, Kanako Higuchi, Masayuki Oohira,
0HJXPL0DH=LDXGGLQ60$WVXWRVKL<RVKLPXUD'HSDUWPHQWRI3HULRGRQWRORJ\DQG(QGRGRQWRORJ\1DJDVDNL8QLYHUVLW\*UDGXDWH6FKRRORI%LRPHGLFDO6FLHQFHVBackground and objective: Traumatic occlusion promotes bone resorption at alveolar bone crest and furcation area and
LQGXFHVRVWHRFODVWVLQDUHODWLYHO\VKRUWSHULRG,WZDVUHSRUWHGWKDWFHOOF\FOH±DUUHVWHGTXLHVFHQWRVWHRFODVWSUHFXUVRUV（QOP）SUHH[LVWDWWKHVLWHRIRVWHRFODVWRJHQHVLV（J Cell Biol, 2009）
7KHSXUSRVHRIWKLVVWXG\ZDVWRFODULI\WKHUROHRI
423LQERQHUHVRUSWLRQFDXVHGE\WUDXPDWLFRFFOXVLRQMaterials and Methods: 2FFOXVDOWUDXPDZDVLQGXFHGLQWKHORZHUOHIW¿UVWPRODURIPLFHE\ERQGLQJDZLUHWRWKHXSSHUOHIW¿UVWPRODU7KHPLFHZHUHLQMHFWHGLQWUDSHULWRQHDOO\ZLWKEURPRGHR[\XULGLQH（BrdU）, which can be incorporated into
GLYLGLQJQXFOHL7KHQXPEHURIRVWHRFODVWVDQGSHUFHQWDJHRI%UG8‑positive nuclei in total osteoclasts nuclei were counted
after tartrate‑resistant acid phosphatase（75$3）DQG%UG8GRXEOHODEHOLQJ423ZDVGHWHFWHGDV75$3‑positive and
BrdU‑QHJDWLYHFHOOVResults: Following the application of traumatic occlusion for 2 days, hyalinized tissue appeared in the furcation area at the¿UVWPRODULQWKHPLFH2QGD\PDQ\RVWHRFODVWVZHUHREVHUYHGDWWKHWRSRIWKHLQWHU‑UDGLFXODUVHSWXP$IHZ75$3‑
positive and BrdU‑QHJDWLYHFHOOVHPHUJHGLQWKHIXUFDWLRQDUHDRQGD\DQGLQFUHDVHGXQWLOGD\2QGD\75$3‑positive
and BrdU‑QHJDWLYHFHOOVZHUHIRXQGLQWKHIXUFDWLRQDUHDConclusion: QOP accumulated in the furcation area immediately after the application of traumatic occlusion, suggesting that
423SOD\HGDQLPSRUWDQWUROHLQWKHUDSLGDOYHRODUERQHUHVRUSWLRQFDXVHGE\WUDXPDWLFRFFOXVLRQJSP/JACP Poster Session AAP2020

11

GB‑21

$GYDQFHGJO\FDWLRQHQGSURGXFWVSURPRWHWKH&2;H[SUHVVLRQRIRVWHREODVWVKeiko Tomita＊11DWVXNR7DQDEH2, Takashi Kato11RUL\XNL$UDL1 1DRWR6X]XNL2, Shuichi Sato1
1'HSDUWPHQWRI3HULRGRQWRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\2'HSDUWPHQWRI%LRFKHPLVWU\1LKRQ
University School of Dentistry

Background and objective: 3HULRGRQWLWLVKDVEHHQVKRZQFRUUHODWLRQZLWKYDULRXVPHWDEROLFV\QGURPH3DUWLFXODUO\DQ
epidemiological study showed that diabetes is associated with periodontitis（'HPPHUHWDO）$GYDQFHGJO\FDWLRQHQG
products（AGEs）DUHIRUPHGE\EHWZHHQWKHH[FHVVVXJDUVDQGSURWHLQVSURGXFHGIURPK\SHUJO\FHPLFGLVRUGHUVVXFKDV
GLDEHWHV7KXVWKHLQYROYHPHQWRIDGYDQFHGJO\FDWLRQHQGSURGXFWV（AGEs）has been focused as an important factor of
GLDEHWHV +RZHYHU WKH PHFKDQLVP RI$*(V HIIHFWV RQ SHULRGRQWLWLV DUH QRW ZHOO XQGHUVWRRG:H WKHUHIRUH DLPHG WR
LQYHVWLJDWHWKHH൵HFWRI$*(VRQWKHH[SUHVVLRQRIF\FORR[\JHQDVH（COX）which is the synthetase of prostaglandin E2 is a
PDMRUIDFWRURILQÀDPPDWLRQWKDWFDXVHVWKHSURGXFWLRQRI5$1./
Materials and Methods: MC3T3‑E1 cells were stimulated with LPS（10 ng/ml）and AGEs（100 ȝg/ml）
 P51$
H[SUHVVLRQRI&2;&2;DQG5$*(ZDVGHWHUPLQHGRQGD\VDQGRIFXOWXUHXVLQJUHDO‑WLPH3&5
Results: /36$*(LQFUHDVHGP51$OHYHOVRI&2;RQGD\RIFXOWXUHFRPSDUHGWR/36DORQH/36$*(DOVRLQFUHDVHGP51$OHYHOVRI5$*(RQGD\RIFXOWXUHFRPSDUHGWR/36DORQH+RZHYHU/36$*(GLGQRWD൵HFWP51$
OHYHOVRI&2;RQGD\RIFXOWXUH
Conclusion: These results suggest that AGEs affect LPS‑LQGXFHG LQIODPPDWLRQ RI RVWHREODVWV$*(V PD\ SURPRWH
LQÀDPPDWRU\ERQHUHVRUSWLRQ

GB‑22(൵HFWRIWRSLFDO37+DGPLQLVWUDWLRQLQUDWFDOYDULDO*%5PRGHO
Akira Hasuike＊1,2, Tatsuya Kubota1, Keiko Tomita1.HQML7DQDND 1<DVXR7VXML1, Shuichi Sato1,2
1'HSDUWPHQWRI3HULRGRQWRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\2Division of Advanced Dental Treatment,'HQWDO5HVHDUFK&HQWHU1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\

Background and objective: ,QWHUPLWWHQWDGPLQLVWUDWLRQRI37+（1‑34）is one of common treatments of osteoporosis, andQRWRQO\LQFUHDVHVWKHERQHGHQVLW\EXWDOVRSURPRWHVRVWHRJHQHVLV,QSUHYLRXVVWXGLHVZHKDYHVKRZQWKDWLQWUDSHULWRQHDO
administration of PTH（1‑34）SURPRWHV YHUWLFDO ERQH DXJPHQWDWLRQ LQ FDOYDULDO *%5 PRGHO RI RYDULHFWRPL]HG
RVWHRSRURVLVPRGHOUDWV,QWKLVVWXG\ZHLQYHVWLJDWHGWKHH൵HFWRIORFDODGPLQLVWUDWLRQRI37+（1‑34）on the same animal
PRGHO
Materials and Methods: At 6 weeks of age, ovariectomy（OVX‑PTH rats）or Sham operation（control rats）was
SHUIRUPHG$WZHHNVRIDJHSODVWLFFDSVZHUHSODFHGRQFDOYDULDRIERWKJURXSUDWVIROORZLQJERQHPDUURZSHQHWUDWLRQ,QWKH29;‑PTH rats, PTH（1‑34）（35 ȝl / kg）was locally administered to the calvaria three times a week, the same
DPRXQWRIVDOLQHZDVORFDOO\DGPLQLVWHUHGWRFRQWUROUDWV0LFUR‑CT images were taken every other week until week 12, andquantitative analysis of new bone‑OLNHWLVVXHZDVSHUIRUPHG$WZHHNVKLVWRFKHPLFDOVHFWLRQVZHUHSUHSDUHGDQG
LPPXQRVWDLQHG
Results: Formation of newly bone‑like tissue was observed in both the groups in micro‑&77KHUHZDVQRVLJQLILFDQWGL൵HUHQFHEHWZHHQWZRJURXSVLQWKHDPRXQWRIQHZO\IRUPHGERQH‑OLNHWLVVXH+LVWRORJLFDOREVHUYDWLRQVKRZHGWKDWPDQ\EORRGYHVVHOVRVWHREODVWVDQGRVWHRFODVWVZHUHIRXQGLQWKHQHZERQHLQERWKJURXSVE\+(VWDLQLQJConclusion,WZDVVKRZHGWKDWLQWHUPLWWHQWWRSLFDODGPLQLVWUDWLRQRI37+（1‑34）WRWKH*%5VLWHUHGXFHGWKHH൵HFWVRI
HVWURJHQGH¿FLHQF\RQERQHDXJPHQWDWLRQDQGUHVWRUHGERQHJURZWKWRKHDOWK\OHYHOVJSP/JACP Poster Session AAP2020

12

GB‑23

&ROODJHQPHPEUDQHDV*%5PDWHULDOLQUDWYHUWLFDODXJPHQWDWLRQPRGHO
Shuichi Sato＊1,2, Yasumasa Ozawa1,2, Tatsuya Kubota1+LVDVKL8MLLH1,
Kazunari Ando1, Akira Hasuike1,2
1'HSDUWPHQWRI3HULRGRQWRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\2Division of Advanced Dental Treatment,'HQWDO5HVHDUFK&HQWHU1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\

Background and objective: ,Q*%5（*XLGHG%RQH5HJHQHUDWLRQ）procedure, a space for bone regeneration is secured by
XVLQJ*%5EDUULHUV5HFHQWO\KLJKO\SHUPHDEOHPDWHULDOVVXFKDVDEVRUEDEOHPHPEUDQHVKDYHEHHQFOLQLFDOO\XVHGIRU
*%5 ,Q WKLV VWXG\ WKH HIIHFWV RI FROODJHQ PHPEUDQH IRU *%5 ZHUH H[DPLQHG E\ ERWK UDGLRJUDSKLF DQG KLVWRORJLFDO
DVVHVVPHQWVLQUDWFDOYDULD
Materials and Methods: Ten‑week‑old male rats（)MFO）ZHUHXVHG7KHFDOYDULDRIUDWVZHUHH[SRVHGDQGSODVWLF
SLSHVZHUHSODFHGELODWHUDOO\(DFKSLSHZDV¿OOHGZLWK%LR‑Oss®, and collagen membranes（Bio‑Gide®）were put on thehalf of plastic pipes as lid（BG）7KHRWKHUVZHUHNHSWZLWKQRWRSV（OP）
6XUJHU\ZDVSHUIRUPHGDWZHHNDQGLPDJHV
were taken up to 12 weeks by using with micro‑computed tomography（micro‑CT）,QDGGLWLRQSDUD൶Q‑embedded andresin‑HPEHGGHGVHFWLRQVDWZHHNVZHUHDVVHVVHGKLVWRORJLFDOO\Results: Formation of newly bone‑like tissue was observed in both the groups in micro‑&77KHUHZDVQRVLJQLILFDQWGL൵HUHQFHEHWZHHQWZRJURXSVLQWKHDPRXQWRIQHZO\IRUPHGERQH‑OLNHWLVVXH+LVWRORJLFDOREVHUYDWLRQVKRZHGWKDWQHZ
bone formation was observed surrounding Bio‑2VVSDUWLFOHVDWZHHNVLQERWKJURXSV+RZHYHULQ¿OWUDWLRQDUHDRI¿EURXVFRQQHFWLYHWLVVXHZDVFRQ¿UPHGLQWKHXSSHUSDUWLQVLGHRISLSHVLQWKH23JURXS
Conclusion$OWKRXJKLQ¿OWUDWLRQRI¿EURXVVRIWWLVVXHZDVODUJHLQ23JURXSVQHZO\IRUPHGERQHWLVVXHZDVFRQ¿UPHGLQ
UDWFDOYDULD*%5PRGHOPDGHZLWKKLJKSHUPHDEOHEDUULHUGB‑24

3URLQÀDPPDWRU\QDWXUHRIDVSLUDWHGSHULRGRQWRSDWKLFEDFWHULDPD\FDXVHSQHXPRQLD
1RULKLVD:DWDQDEH＊1, Sho Yokoe1.HQLFKL,PDL2, Shuichi Sato1
1'HSDUWPHQWRI3HULRGRQWRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\2'HSDUWPHQWRI0LFURELRORJ\1LKRQ
University School of Dentistry

Background and objective: $VSLUDWLRQSQHXPRQLDLVDPDMRUKHDOWKSUREOHPRZLQJWRLWVKLJKPRUWDOLW\UDWHLQHOGHUO\SHRSOH7KHVHFUHWLRQRISURLQÀDPPDWRU\F\WRNLQHVVXFKDV,/‑DQG,/‑6 by respiratory epithelial cells, which is induced
by infection of respiratory bacteria such as Streptococcus pneumoniae（S.p）FRQWULEXWHVWRWKHRQVHWRISQHXPRQLD7KHVH
F\WRNLQHVWKXVSOD\DNH\UROHLQRUFKHVWUDWLQJLQÀDPPDWRU\UHVSRQVHVLQWKHORZHUUHVSLUDWRU\WUDFW$OWKRXJKHPHUJLQJ
evidence has revealed an association between aspiration pneumonia and periodontitis, how periodontitis contributes to the
RQVHWRIDVSLUDWLRQSQHXPRQLDUHPDLQVXQFOHDU0RVWSHULRGRQWRSDWKLFEDFWHULDDUHDQDHURELFDQGDUHWKHUHIRUHXQOLNHO\WR
VXUYLYHLQWKHORZHUUHVSLUDWRU\RUJDQVRIKXPDQV+HQFHZHDLPHGWRHOXFLGDWHZKHWKHUH[SRVXUHWRKHDW‑inactivated
SHULRGRQWRSDWKLFEDFWHULDLQGXFHVSURLQÀDPPDWRU\F\WRNLQHSURGXFWLRQE\VHYHUDOKXPDQUHVSLUDWRU\HSLWKHOLDOFHOOVDQGLQWKHORZHUUHVSLUDWRU\RUJDQVDQGVHUXPLQPLFH
Results: Periodontopathic bacteria, especially Fusobacterium nucleatum（F.n）VWURQJO\LQGXFHG,/‑DQG,/‑6 production
by BEAS‑%EURQFKLDOHSLWKHOLDOFHOOV,QDGGLWLRQF.nLQGXFHG,/‑8 production by A549 alveolar epithelial cells as well as,/‑DQG,/‑SURGXFWLRQE\'HWURLWSKDU\QJHDOHSLWKHOLDOFHOOV)XUWKHUPRUHF.n induced considerably higher cytokine
production than S.p7KLVZDVDOVRREVHUYHGLQWKHHQWLUHORZHUUHVSLUDWRU\RUJDQVDQGVHUXPLQPLFH
Conclusion: F.nLVDSRZHUIXOLQÀDPPDWRU\VWLPXODQWIRUUHVSLUDWRU\HSLWKHOLDOFHOOVDQGFDQVWLPXODWHF\WRNLQHSURGXFWLRQWKHUHE\SRWHQWLDOO\FRQWULEXWLQJWRWKHRQVHWRIDVSLUDWLRQSQHXPRQLD

JSP/JACP Poster Session AAP2020

13

GB‑25

/DVHULUUDGLDWLRQGHFUHDVHV6RVWH[SUHVVLRQLQERQHDQGRVWHRJHQLFFHOOV<XMLQ2KVXJL＊1, Sayaka Katagiri1, Tomomitsu Hirota2+LURPL1LLPL1, Masahiro Hatasa1,.D]XNL:DWDQDEH1, Tsuyoshi Shimohira1.RML0L]XWDQL1, Moe Kitazawa3, Ayumi Matsuzawa3,
Hiroshi Kadokura4, Satoshi Yokose47DNDQRUL,ZDWD1, Akira Aoki1
1

Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental
University, 2'HSDUWPHQWRI0ROHFXODU*HQHWLFV5HVHDUFK&HQWHUIRU0HGLFDO6FLHQFH7KH-LNHL8QLYHUVLW\6FKRRO
of Medicine, 3'HSDUWPHQWRI(SLJHQHWLFV0HGLFDO5HVHDUFK,QVWLWXWH7RN\R0HGLFDODQG'HQWDO8QLYHUVLW\4'LYLVLRQRI(QGRGRQWLFDQG2SHUDWLYH'HQWLVWU\'HSDUWPHQWRI5HVWRUDWLYHDQG%LRPDWHULDOV6FLHQFHV6FKRRORI
Dentistry, Meikai University
Background and objective: Er: YAG laser has been applied for bone ablation in both periodontal and peri‑LPSODQWVXUJHU\+RZHYHUWKHH൵HFWVRI(U<$*ODVHULUUDGLDWLRQRQERQHWLVVXHUHPDLQXQFOHDU7KHSXUSRVHRIWKLVVWXG\ZDVWRHYDOXDWHFRPSUHKHQVLYHDQGVHTXHQWLDOJHQHH[SUHVVLRQLQ(U<$*ODVHU‑ablated, bur‑drilled, and non‑treated control bones to
FODULI\WKHELRORJLFDOUHVSRQVHV)XUWKHUPRUHELR‑VWLPXODWLRQH൵HFWRI(U<$*ODVHURQRVWHRJHQLFFHOOVZDVLQYHVWLJDWHG
Materials and Methods: The calvarial bone of male, 10‑week‑ROG :LVWDU UDWV ZDV DEODWHG E\ (U<$* ODVHU DQGFRQYHQWLRQDOEXU*HQHH[SUHVVLRQLQWKHODVHU‑ablated, bur‑drilled, and non‑treated control bones at 6, 24, and 72 h wereHYDOXDWHGE\PLFURDUUD\2VWHREODVW‑OLNHFHOOVZHUHLVRODWHGIURPWKHFDOYDULDRI±‑days‑ROG:LVWDUUDWVDQGFXOWXUHGZLWKRVWHRLQGXFWLRQPHGLXPWRLQGXFHRVWHRJHQLFFHOOV2VWHRJHQLFFHOOVZHUHLUUDGLDWHGXVLQJ(U<$*ODVHUDWHQHUJ\ÀXHQFHVRIDQG-FP2
Results: *HQHH[SUHVVLRQSDWWHUQVZHUHFOHDUO\GL൵HUHQWDPRQJQRQ‑treated, bur‑drilled, and laser‑irradiated bone tissues at
KRXUV*HQH6HW(QULFKPHQW$QDO\VLVVKRZHGLQÀDPPDWLRQ‑related gene sets were enriched in the bur‑drilled bone at 6h, whereas these gene sets were enriched in the laser‑DEODWHGERQHDWKFRPSDUHGWRWKDWRIWKHFRQWUROERQH(U<$*
laser irradiation suppressed SostH[SUHVVLRQERWKLQERQHWLVVXHDQGRVWHRJHQLFFHOOV'L൵HUHQWLDOO\H[SUHVVHGJHQHVEHWZHHQ
bur‑drilled and laser‑DEODWHGERQHVKRZHGWKDWOHVVLQÀDPPDWLRQZDVLQGXFHGLQWKHODVHU‑DEODWHGERQH
Conclusion: Our study suggests that bone ablation with Er: YAG laser may stimulate osteocytes in the bone tissue and
LQGXFHDGYDQWDJHRXVUHVSRQVHVLQQHZERQHIRUPDWLRQGB‑26,QYROYHPHQWRI&;&5LQDOYHRODUERQHUHVRUSWLRQLQSHULRGRQWLWLV‑PLFH+LGHND]X1DJDVKLPD＊11DRNL0XUDNDPL1,2, Hariu Kotaro1, Takane Masatoshi1,
Masamichi Shinoda2.RLFKL,ZDWD2 , Shuichi Sato1
1'HSDUWPHQWRI3HULRGRQWRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\2'HSDUWPHQWRI3K\VLRORJ\1LKRQ
University School of Dentistry

Background and objective: 3HULRGRQWLWLVFDXVHGE\EDFWHULDOLQIHFWLRQSURJUHVVHVSHULRGRQWDOWLVVXHGHVWUXFWLRQOLWWOHE\OLWWOH
$VDUHVXOWWHHWKORVHWKHLUVXSSRUWLQJVWUXFWXUHVDQGWKLVOHDGVWRWKHORVVRIDWRRWK&;&‑chemokine receptor 4（&;&5）
LVNQRZQWREHH[SUHVVHGLQO\PSKRF\WHVILEUREODVWVDQGRVWHRFODVWVLQSHULRGRQWDOWLVVXHVVXJJHVWLQJWKDWSHULRGRQWDO
&;&5VLJQDOLQJFRQWULEXWHVWRDOYHRODUERQHUHVRUSWLRQDWWKHSHULRGRQWLWLV+RZHYHUWKHUROHRI&;&5VLJQDOLQJLQWKH
SDWKRJHQHVLVRISHULRGRQWLWLVKDVUHPDLQHGXQNQRZQMaterials and Methods: :HHVWDEOLVKHGDPRXVHPRGHORISHULRGRQWLWLVE\OLJDWLQJDVLONWKUHDGWRWKHXSSHUPRODUDQG
inoculating Porphyromonas gingivalis（P.g.）,QRUGHUWRFRQ¿UPZKHWKHUWKLVSHULRGRQWLWLVPRGHOSURJUHVVZLWKRXWSDLQWKHPHFKDQLFDOSDLQWKUHVKROGDWSHULRGRQWDOWLVVXHZDVFRPSDUHGZLWKSRVLWLYHFRQWUROXVLQJ&)$)XUWKHUPRUHD&;&5
neutralizing antibody was administered to periodontal tissue after P.g. inoculation, bone resorption was observed in the
H[SHULPHQWDOSHULRGXVLQJȝ‑&7DQGWLVVXHVWDLQLQJZDVSHUIRUPHGWRREVHUYHFHOOVLQ¿OWUDWHGORFDOO\Results: ,WZDVLQGLFDWHGWKDWWKLVSHULRGRQWLWLVPRGHOXVLQJP.g.SURJUHVVZLWKQRSDLQLQFUHDVH,QWKLVSHULRGRQWLWLVPRGHO
DGPLQLVWUDWLRQRI&;&5QHXWUDOL]LQJDQWLERG\HQKDQFHGLQÀDPPDWRU\FHOOLQ¿OWUDWLRQLQSHULRGRQWDOWLVVXHRQGD\RI
WKHH[SHULPHQWDOSHULRGVDQGHQKDQFHGERQHUHVRUSWLRQRQGD\VWR
Conclusion: 7KHVH¿QGLQJVVXJJHVWWKDWSHULRGRQWDO&;&5VLJQDOLQJLQVHYHUDOW\SHRILPPXQHFHOOVLQWKHVLWXDWLRQRI
periodontitis induced by P.g. GHSUHVVHV DOYHRODU ERQH UHVRUSWLRQ &;&5 VLJQDOLQJ PLJKW EH DWDUJHW IRU WKHUDSHXWLF
LQWHUYHQWLRQWRSUHYHQWDOYHRODUERQHUHVRUSWLRQLQSHULRGRQWLWLVJSP/JACP Poster Session AAP2020

14

GB‑27

7KHH൵HFWVRI(U<$*ODVHULUUDGLDWLRQRQSULPDU\RVWHREODVW‑OLNHFHOOV+LURPL1LLPL＊1<XMLQ2KVXJL1, Sayaka Katagiri1.D]XNL:DWDQDEH1, Masahiro Hatasa1,
Tsuyoshi Shimohira1, Shogo Maekawa1, Tomomitsu Hirota2, Hiroshi Kadokura3,
Satoshi Yokose37DNDQRUL,ZDWD1, Akira Aoki1
1

Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental
University, 2'HSDUWPHQWRI0ROHFXODU*HQHWLFV5HVHDUFK&HQWHUIRU0HGLFDO6FLHQFHV7KH-LNHL8QLYHUVLW\6FKRRO
of Medicine, 3'LYLVLRQRI(QGRGRQWLFDQG2SHUDWLYH'HQWLVWU\'HSDUWPHQWRI5HVWRUDWLYHDQG%LRPDWHULDOVSciences, School of Dentistry, Meikai University
Background and objective: $QXPEHURIUHSRUWVKDYHVKRZQWKDWYDULRXVODVHUVKDYHIDYRUDEOHELRORJLFDOH൵HFWVRQFHOOV+RZHYHUWKHH൵HFWVRIORZ‑OHYHO(U<$*ODVHULUUDGLDWLRQRQRVWHREODVWVUHPDLQXQFOHDU7KHSXUSRVHRIWKLVVWXG\ZDVWR
evaluate the effects of low‑level Er: YAG laser irradiation on proliferation and osteogenic differentiation of primaryosteoblast‑OLNHFHOOV
Materials and Methods: Osteoblast‑OLNHFHOOVLVRODWHGIURPWKHFDOYDULDHRI±‑day‑ROG:LVWDUUDWVZHUHLUUDGLDWHGE\(U<$*ODVHUDWHQHUJ\ÀXHQFHVRIRU-FP2&HOOVXUIDFHWHPSHUDWXUHZDVPHDVXUHGDQGFHOOSUROLIHUDWLRQZDVHYDOXDWHG&DOFL¿FDWLRQZDVHYDOXDWHGE\PHDVXULQJWKH$OL]DULQUHG6VWDLQLQJDUHDDIWHU‑day‑culture with osteoinductive
PHGLXP*HQHH[SUHVVLRQVLQQRQ‑irradiated and laser‑LUUDGLDWHGFHOOVZHUHHYDOXDWHGE\T3&5DWDQGKRXUVDIWHULUUDGLDWLRQ0LFURDUUD\DQDO\VLVZDVSHUIRUPHGWRFRPSUHKHQVLYHO\HYDOXDWHJHQHH[SUHVVLRQVRIQRQ‑irradiated cells and
LUUDGLDWHGFHOOVDW-FP2DWKRXUVDIWHULUUDGLDWLRQResults: 1RSURQRXQFHGLQFUHDVHRIFHOOVXUIDFHWHPSHUDWXUHZDVLQGXFHGE\ORZ‑level Er: YAG laser irradiation, and the
LUUDGLDWLRQGLGQRWD൵HFWRVWHREODVW‑OLNHFHOOSUROLIHUDWLRQ&DOFL¿FDWLRQZDVVLJQL¿FDQWO\LQFUHDVHGGD\VDIWHULUUDGLDWLRQDW-FP2BglapH[SUHVVLRQZDVVLJQL¿FDQWO\LQFUHDVHGLQFHOOVLUUDGLDWHGDW-FP2 at 6 hours post‑LUUDGLDWLRQ0LFURDUUD\DQDO\VLVVKRZHGWKDWLUUDGLDWLRQDW-FP2 up‑UHJXODWHGRILQÀDPPDWLRQ‑related genes and down‑regulated of Wisp2
*HQHVHWHQULFKPHQWDQDO\VLVFODUL¿HGHQULFKPHQWRILQÀDPPDWLRQ‑UHODWHGDQG1RWFKVLJQDOLQJJHQHVHWVConclusion: Low‑OHYHO(U<$*ODVHULUUDGLDWLRQHQKDQFHGFDOFL¿FDWLRQRISULPDU\RVWHREODVW‑like cells via enhanced Bglap
H[SUHVVLRQDQGHQULFKHG1RWFKVLJQDOLQJ
GB‑28(൵HFWVRI3RUSK\URPRQDVJLQJLYDOLV/36RQFDUGLDFIXQFWLRQLQPLFH,FKLUR0DWVXR＊
Department of Periodontology, Tsurumi University School of Dental Medicine
Background and objective: 3HULRGRQWLWLVZDVUHFHQWO\UHSRUWHGWRLQGXFHDFWLYDWLRQRIV\PSDWKHWLFQHUYRXVV\VWHPV2QWKH
other hand, chronic activation of sympathetic activity is known to induce the development of cardiac remodeling and heart
IDLOXUH:HWKXVH[DPLQHGLQPLFHWUHDWHGZLWKOLSRSRO\VDFFKDULGHGHULYHGIURPPorphylomonas gingivalis（PG‑LPS）at a
dose equivalent to the circulating levels in patients with periodontitis with/without ß‑adrenergic receptor（ß‑$5）blocker
propranolol（PPL）
Materials and Methods: Mice（C57BL6/J）were divided into four groups: 1）Control group, 2）LPS group（PJNJGD\LS）, 3）PPL group（via the drinking water containing 1g/L）, and 4）33//36JURXSResults: $IWHUZHHNZHH[DPLQHGWKHFDUGLDFIXQFWLRQXVLQJHFKRFDUGLRJUDSK\&DUGLDFIXQFWLRQDVH[HPSOL¿HGE\WKHOHIWYHQWULFXODUFDUGLDFIXQFWLRQZDVVLJQL¿FDQWO\GHFUHDVHGE\WKHWUHDWPHQWRI3*‑LPS（Control（n=6）YV/36（n=6）: 66 ±YV±％, P＜YV&RQWURO）
+RZHYHUFR‑treatment of PPL suppressed the decrease of PG‑LPS‑mediated cardiac
dysfunction（62 ±％ , n=5, P＜YV/36JURXS）
7KHQXPEHURIDSRSWRWLFP\RF\WHVZDVLQFUHDVHG（DSSUR[LPDWHO\$JDLQWKHVHLQFUHDVHVZHUHDEURJDWHGE\WKHFR‑
10‑fold）DQGWKHDUHDRI¿EURVLVZDVLQFUHDVHG（DSSUR[LPDWHO\‑fold）
WUHDWPHQWRI33/7KHLPSDLUPHQWRIFDUGLDFIXQFWLRQLQ3*‑LPS‑treated mice appears to involve dysregulation of Ca2+
homeostasis in cardiac myocytes induced by ß‑$5DFWLYDWLRQDQGLWVGRZQVWUHDPPROHFXOHVYLD3*‑/36
Conclusion: Our results indicated that ß‑$5VLJQDOLQJPLJKWEHQHZWKHUDSHXWLFWDUJHWVIRUWUHDWPHQWRIFDUGLRYDVFXODUGLVHDVHLQSDWLHQWVZLWKSHULRGRQWLWLVJSP/JACP Poster Session AAP2020

15

GB‑29

/,386VWLPXODWLRQSURPRWHVFROODJHQV\QWKHVLVYLD3;UHFHSWRU
Soichiro Manaka＊1DWVXNR7DQDEH.HLNR7RPLWD<RVKLWRPR0RUL\D(LML*RNH1DRWR6X]XNL6KXLFKL6DWR11LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\
Background and objective: Low‑intensity pulsed ultrasound（/,386）is a clinically applied medical device in the medical
DQGGHQWDO¿HOGV,QDGGLWLRQLWKDVEHHQUHSRUWHGWKDW3;UHFHSWRUDUHFHSWRUIRUDGHQRVLQHWULSKRVSKDWHLVLQYROYHGLQ
ERQH IRUPDWLRQE\PHFKDQLFDOVLJQDOLQJ)XUWKHUW\SH,FROODJHQ（&RO,）LV RQH RI WKH PDMRU SURWHLQ FRPSRQHQWV RI
H[WUDFHOOXODUPDWUL[SURWHLQSURGXFHGLQERQHWLVVXHDQGLVSDUWLFXODUO\LQYROYHGLQRVWHREODVWLFERQHIRUPDWLRQ+RZHYHU
WKHLQWHUDFWLRQEHWZHHQ/,386‑LQGXFHG$73DQGFROODJHQPHWDEROLVPDWWKH3;UHFHSWRUKDVQRWEHHQFODUL¿HG,QWKLVVWXG\ZHH[DPLQHGWKHUROHRI3;UHFHSWRURQFROODJHQPHWDEROLVPLQ/,386‑induced bone formation by cell biological
DQGPROHFXODUELRORJ\Materials and Methods: Osteoblastic MC3T3‑E1 cells were seeded on a 6 well plate and cultured in the presence or absence
RIWKHVHOHFWLYH3;UHFHSWRULQKLELWRU$IROORZHGE\PLQGD\RI/,386VWLPXODWLRQXVLQJ267(27521'2,Q
DGGLWLRQWKHVHFHOOVWUDQVIHFWHGZLWK3;VK51$（P2X7 knockdown cells；shP2X7）were also prepared and stimulated
ZLWK/,386XQGHUWKHVDPHFRQGLWLRQV7KHSURWHLQH[SUHVVLRQRI&RO,DQGPDWUL[PHWDOORSURWHLQDVHV（MMPs）was
GHWHUPLQHGXVLQJ(/,6$
Results: ,QERWKRVWHREODVWLF0&7‑E1 cells in the presence of A438079 and their P2X7 knockdown cells（shP2X7）,/,386VWLPXODWLRQUHGXFHGWKHSURWHLQH[SUHVVLRQRI&RO,ZKHUHDVLQGXFHGWKHSURWHLQH[SUHVVLRQRI003‑DQG
Conclusion: 7KHVHUHVXOWVVXJJHVWWKDW/,386SURPRWHVRVWHRJHQHVLVE\VWLPXODWLQJRVWHREODVWLFFROODJHQPHWDEROLVPWRWKHV\QWKHWLFV\VWHPYLDWKH3;UHFHSWRU

GB‑30

7KHH൵HFWRIVZRUGEHDQH[WUDFWRQSHULRGRQWLWLVDQGDWKHURVFOHURVLVLQPLFH
Shunsuke Yanase＊1, Kosei Matsumoto1, Ayuko Takada2, Yasushi Furuichi1
1'HSDUWPHQWRI2UDO5HKDELOLWDWLRQ'LYLVLRQRI3HULRGRQWRORJ\DQG(QGRGRQWRORJ\6FKRRORI'HQWLVWU\+HDOWKSciences University of Hokkaido, 2Division of Biochemistry, Department of Oral Biology, Health Sciences
University of Hokkaido

Background and objective: 5HFHQWO\VHYHUDOVWXGLHVKDYHVXJJHVWHGWKDWSHULRGRQWLWLVDFFHOHUDWHVDWKHURVFOHURVLV（AC）
2XUODERUDWRU\UHSRUWHGWKDWVZRUGEHDQVH[WUDFW（SBE）KDVH൵HFWVIRUDQWL‑LQÀDPPDWRU\DQGDQWL‑bacterial in periodontal
WLVVXH7KHSXUSRVHRIWKLVVWXG\ZDVWRH[DPLQHWKHH൵HFWRI6%(RQ$&DFFHOHUDWHGE\SHULRGRQWLWLV
Materials and Methods: 6L[‑weeks‑old male ApoE‑/‑ mice were induced periodontitis by oral P. gingivalis infection（P. g）
or 5‑0 silk ligature tied around the molars（Lig）
7KH\ZHUHUDQGRPO\GLYLGHGLQWRVL[JURXSVDVIROORZV① AC（Control）,② P. g, ③ Lig, ④ SBE（added to drinking water starting at 7 weeks of age）
, ⑤ P. g+SBE, ⑥ /LJ6%($OOWKHPLFHIHG
KLJKIDWGLHW$WWKHDJHRIZHHNVDOOWKHPLFHZHUHVDFUL¿FHG3HULRGRQWDOWLVVXHDQGDRUWDZHUHHYDOXDWHGKLVWRORJLFDOO\DQGPRUSKRORJLFDOO\7KHJHQHH[SUHVVLRQVRIL126,/‑DQG71)‑ĮLQWKHKHDUWWLVVXHZHUHHYDOXDWHG
Results: 7KHDPRXQWRIDOYHRODUERQHORVVLQWKH/LJ6%(ZDVVLJQL¿FDQWO\ORZHUWKDQWKDWRIWKH/LJ,QDQDO\VLVRIWKH
cross sections in aorta, P. g ＋ SBE and Lig ＋ 6%(ZHUHVLJQL¿FDQWO\ORZHULQDUHDRIDWKHURVFOHURVLVWKDQP. gDQG/LJ*HQHH[SUHVVLRQRIL126DQG71)‑Į in the heart of P. g ＋ 6%(ZHUHVLJQL¿FDQWO\ORZHUWKDQWKDWRIP. g
Conclusion: This study indicated that SBE down‑UHJXODWHG L126 JHQH H[SUHVVLRQ DQG LQKLELWHG SURJUHVVLRQ RI$&DFFHOHUDWHGE\SHULRGRQWLWLV,WZDVVXJJHVWHGWKDW6%(FRXOGEHDSSOLHGDVDWKHUDSHXWLFDJHQWIRU$&

JSP/JACP Poster Session AAP2020

16

GB‑31

/,386HQKDQFHVUK%03‑LQGXFHGERQHIRUPDWLRQLQUDWFDOYDULDOERQHGHIHFWV
7DNDWRPR,PDIXML＊, Yoshinori Shirakata, Yukiya Shinohara,
7RVKLDNL1DNDPXUD.D]X\XNL1RJXFKLDepartment of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences
Background and objective: ,W KDV UHFHQWO\ EHHQ UHSRUWHG WKDW ERQH PRUSKRJHQHWLF SURWHLQ（BMP）‑9 has great
osteoinductive properties in pre‑FOLQLFDO VWXGLHV LQ YDULRXV FUDQLRIDFLDOSHULRGRQWDO ERQH GHIHFWV %RQH UHSDLU LV DOVRUHJXODWHGE\ORFDOPHFKDQLFDOVWLPXODWLRQ7KHUHIRUHWKHDLPRIWKLVVWXG\ZDVWRH[DPLQHWKHFRPELQHGH൵HFWVRI/RZ‑
intensity pulsed ultrasound（/,386）and recombinant human（rh）BMP‑RQERQHKHDOLQJLQUDWFDOYDULDOGHIHFWVMaterials and Methods: &LUFXODUFDOYDULDOGHIHFWVZHUHVXUJLFDOO\FUHDWHGLQZLVWDUUDWV7KHDQLPDOVZHUHGLYLGHGLQWR
two groups（/,386‑applied（+）DQG/,386‑non‑applied（‑））and the 36 defects in each group randomly received thefollowing treatments: absorbable collagen sponge（ACS）（φPP）implantation（ACS）
$&6ZLWKJUK%03‑9（rhBMP9/ACS）and empty control（control）
$VDUHVXOWWKHIROORZLQJJURXSV（control（+）/（‑）, ACS（+）/（‑）,
and rhBMP9/ACS（+）/（‑））ZHUH HYDOXDWHG7KH /,386‑DSSOLHG JURXS UHFHLYHG GDLO\ /,386 H[SRVXUH VWDUWLQJ
LPPHGLDWHO\DIWHUWKHVXUJHU\$WZHHNVWKHDQLPDOVZHUHVDFUL¿FHGIRUPLFUR‑&7DQGKLVWRORJLFHYDOXDWLRQResults: 3RVWRSHUDWLYHFOLQLFDOKHDOLQJZDVXQHYHQWIXODWDOOVLWHV0RUHQHZERQHZDVREVHUYHGLQWKH/,386‑applied, newly formed bone area（+）JURXSFRPSDUHGWRWKH/,386‑non‑applied（‑）JURXS7KHERQHYROXPH（±PP3）（1%$）（±PP2）DQG1%$WRWDOGHIHFWDUHD（±％）in the rhBMP9（+）group were significantly
greater than those（±PP3±PP2DQG±％ respectively）in the rhBMP9（−）group（p＜）)XUWKHUPRUHWKHUK%03（+）JURXSVKRZHGWKHKLJKHVWOHYHORIERQHIRUPDWLRQDPRQJDOOWKHJURXSV
Conclusion: 7KHSUHVHQWUHVXOWVVXJJHVWWKDW/,386HQKDQFHVUK%03‑9‑LQGXFHGERQHIRUPDWLRQLQFDOYDULDOGHIHFWVLQUDWVGB‑32%HKDYLRURI0&7‑(FHOOVRQVWURQWLXPGRSHGWLWDQLXPVXUIDFH
Urangoo Sugarbaatar＊1, Takashi Kado17DNDVKL1H]X2)XWDPL7DNHEH1DJDQR2,<XNL,FKLRND1, Kazuhiko Endo2, Yasushi Furuichi1
1

Division of Endodontology and Periodontology, 2Division of Biomaterials and Bioengineering, Department of Oral
5HKDELOLWDWLRQ+HDOWK6FLHQFHV8QLYHUVLW\RI+RNNDLGR

Background and objective: The purpose of the study was to establish a new and simple coating method on the mirror‑¿QLVKHG7LVXUIDFHVWRHQKDQFHWLWDQLXPERQHLQWHJUDWLRQ
Materials and Methods: Commercially pure grade‑WLWDQLXPGLVNVZHUHPHFKDQLFDOO\SROLVKHGXVLQJFROORLGDOVLOLFD7KH
test groups were immersed in 5％1D2&OVROXWLRQIRUKDQGXOWUDVRQLFDOO\FOHDQHGLQGLVWLOOHGGHLRQL]HGZDWHU（'':）,
dipped in 1％ Sr（123）2 solution for 30 seconds, dried with spinning and were subsequently treated with or w/o ultrasonic'':7KHFRQWUROJURXSZDVDV‑SROLVKHGWLWDQLXPGLVNV7KHFRQFHQWUDWLRQVRIYDULRXVLRQVGRSHGLQHDFKVXUIDFHZHUHHYDOXDWHGZLWK630VWURQWLXPLRQVFRQFHQWUDWLRQUHOHDVHGZHUHH[DPLQHGZLWK,&3‑2(6DQGZHWWDELOLW\ZDVPHDVXUHG
Afterward, specimens were transferred to 24 well plates and MC3T3‑(FHOOVZHUHVHHGHGDW[3ZHOO$IWHUK
DWWDFKHGFHOOVWRHDFKVXUIDFHZHUHFRXQWHG
Results: 6ULRQVUHOHDVHGRQGD\RIWKH1D2&O‑6UVSHFLPHQVZDVSSPSSPRQGD\DQGZLWKLQZHHNDOPRVWQR6ULRQVZDVGHWHFWHG7KH6ULRQVRIWKH1D2&O‑Sr‑ZDVKHGVSHFLPHQVRQGD\ZDVSSPZKLFKDVRQHRUGHURIPDJQLWXGHORZHU WKDQ WKDW RI WKH 1D2&O‑6U7KHDPRXQWRIUHOHDVHG6ULRQVGHFUHDVHGZLWKWLPH7KHQXPEHURI WKHDWWDFKHGFHOOVRQWKH1D2&O‑6UZDVKLJKHUWKDQRQWKH1D2&O‑Sr‑ZDVKHGVXUIDFH7KH1D2&O‑Sr‑washed and as‑polishedsurfaces were less adhesive than the polystyrene‑GLVKConclusion: 7KH6UGRSHGVPRRWKWLWDQLXPVXUIDFHFRXOGEHFUHDWHGE\VLPSOHFKHPLFDOWUHDWPHQWV7KHVXUIDFHʼs physical
SURSHUWLHVZHUHHQKDQFHGE\6ULQGXFHPHQWJSP/JACP Poster Session AAP2020

17

GB‑33

(൵HFWRIDJLQJRQMXQFWLRQDOPROHFXOHVRIJLQJLYDOHSLWKHOLDOFHOOVSarita Giri＊1, Ayuko Takada20DWVXVKLWD.HQML3, Masae Furukawa3, Yasushi Furuichi1
1'LYLVLRQRI3HULRGRQWRORJ\DQG(QGRGRQWRORJ\'HSDUWPHQWRI2UDO5HKDELOLWDWLRQ6FKRRORI'HQWLVWU\+HDOWKSciences University of Hokkaido, 2Division of Biochemistry, Department of Oral Biology, School of Dentistry,
Health Sciences University of Hokkaido, 3'HSDUWPHQWRI2UDO'LVHDVH5HVHDUFK1DWLRQDO&HQWUHIRU*HULDWULFVDQG
Gerontology
Background and objective: $JLQJFDXVHVSURJUHVVLYHG\VIXQFWLRQLQWKHPHFKDQLFDODQGELRFKHPLFDOIXQFWLRQRIWLVVXHV-XQFWLRQDOPROHFXOHVKDYHFULWLFDOUROHLQPDLQWDLQLQJHSLWKHOLDOKRPHRVWDVLV$JHUHODWHGGHVWUXFWLRQRIJLQJLYDOWLVVXHVPLJKWEHWKHFRQVHTXHQFHVRIDOWHUHGMXQFWLRQDOPROHFXOHV:HDLPHGWRHOXFLGDWHDOWHUHGH[SUHVVLRQRIFODXLQ‑1, 2 andE‑cadherin in young and senescence induced gingival epithelial cells with and w/o Porphyromonas gingivalis
Lipopolysaccharides（Pg LPS）

Materials and Methods: Human gingival epithelial cells（HGEP）ZHUHWUHDWHGZLWKK\GURJHQSHUR[LGH（H2O2）to induce
VHQHVFHQFH0RI+2O2ZDVFRQVLGHUHGDVRSWLPDOFRQFHQWUDWLRQWRLQGXFHVHQHVFHQFHZKLFKZDVFRQ¿UPHGIURPWKH
vitality test, senescence associated ȕ‑JDODFWRVLGDVHVWDLQLQJDQGP51$H[SUHVVLRQRIVHQHVFHQFHPDUNHUVSSDQGSThe cells were divided into four groups；young cells（Control）, young+H2O2（Old）, young+LPS（Y+LPS）and old+LPS（O+LPS）)RU/36WUHDWPHQWFHOOVZHUHWUHDWHGZLWK Pg LPS（1μgmL‑1）7KHP51$DQGSURWHLQH[SUHVVLRQRIMXQFWLRQDO
proteins such as claudin‑1, ‑2 and E‑cadherin were compared using quantitative real time polymerase chain reaction and
ZHVWHUQEORWUHVSHFWLYHO\0DQQ‑:KLWQH\8WHVWZDVXVHGWRWHVWWKHVLJQL¿FDQFH
Results: 7KHP51$H[SUHVVLRQRIFODXGLQ‑1, ‑2 and E‑cadherin were all upregulated in the old cells as compared to young
and Y+LPS cells whereas claudin‑1 and E‑cadherin was downregulated in O+LPS cells as compared to old cells（p＜）
7KHSURWHLQH[SUHVVLRQFRQ¿UPHGE\ZHVWHUQEORWZHUHDOOLQDFFRUGDQFHZLWKWKHP51$H[SUHVVLRQConclusion: $JHUHODWHGDOWHUDWLRQRIMXQFWLRQDOPROHFXOHVPD\EHUHVSRQVLEOHIRUSURJUHVVLYHWLVVXHGHVWUXFWLRQLQHOGHUO\JLQJLYDOWLVVXHV

GB‑34(൵HFWVRIPHFKDQLFDOVWUHVVRQFHOOVGHULYHGIURPKXPDQRUDOPXFRVD
Toshiro Yamamoto＊, Kenta Yamamoto, Tetsuya Adachi, Taku Asai,)XPLVKLJH2VHNR1DULVDWR.DQDPXUD
Department of Dental Medicine, Kyoto Prefectural University of Medicine Graduate School of Medical Science
Background and objective: 7KHRUDOPXFRVDLVH[SRVHGWRPHFKDQLFDOVWUHVV（MS）LQFOXGLQJRFFOXVDOIRUFHYLDWKHGHQWXUH,QWKLVVWXG\ZHDSSOLHG06WRFHOOVGHULYHGIURPKXPDQRUDOPXFRVDDQGHYDOXDWHGLWVH൵HFWVMaterials and Methods: 6XEFRQÀXHQWKXPDQRUDOPXFRVDOHSLWKHOLDOFHOOV（HO‑1‑1‑1）DQGKXPDQJLQJLYDO¿EUREODVWV（HGF）were used to seed 1 105FHOOVGLVKDQGDIWHUKRXUVVXEMHFWHGWR06（1 and 3 MPa, 60 min）using a hydrostatic
SUHVVXUL]HU0RUSKRORJLFDOFKDQJHVRIWKHFHOOVZHUHH[DPLQHGE\*LHPVDVWDLQDQGFKDQJHVLQFHOODFWLYLW\ZHUHPHDVXUHG
E\ WKH:67 DVVD\ P51$ ZDV FROOHFWHG IURP WKH FHOOV DQG LQIODPPDWRU\ F\WRNLQHV DQG JURZWK IDFWRUV JHQHV ZHUH
measured by real‑WLPH57‑3&5DVVD\DQG(/,6$
Results: HO‑1‑1‑VKRZHGQRPRUSKRORJLFDOFKDQJHE\*LHPVDVWDLQEXWH[KLELWHGDVLJQL¿FDQWGHFUHDVHLQFHOODFWLYLW\E\:67DVVD\,Q+2‑1‑1‑WKHH[SUHVVLRQRILQÀDPPDWRU\F\WRNLQHVDQG,&$0P51$ZDVVLJQL¿FDQWO\LQFUHDVHGDIWHU
06DW03DEXWZDVDWWHQXDWHGDW03D,Q+*)WKHH[SUHVVLRQRI,/‑P51$ZDVHQKDQFHG,Q+2‑1‑1‑1 and HGF,
WKH,/‑DQG,/‑SURWHLQOHYHOVLQFUHDVHGVLJQL¿FDQWO\DIWHU06,Q+*)WKH(*)DQG)*)‑2 protein levels increased
VLJQL¿FDQWO\E\VWLPXODWLRQDW03D
Conclusion: MS applied to the oral mucosa caused a decrease in the activity of oral mucosal epithelial cells and induced
HQKDQFHPHQWRILQÀDPPDWRU\F\WRNLQHSURGXFWLRQ$OVRH[FHVVLYH06ZDVVXJJHVWHGWRSURPRWHWKHFXUHRIRUDOPXFRVDZRXQGVE\SURPRWLQJWKHSURGXFWLRQRIJURZWKIDFWRUVLQWKHRUDOPXFRVDJSP/JACP Poster Session AAP2020

18

GB‑35

$QWL‑LQÀDPPDWRU\H൵HFWVRIȕ‑FU\RQ)8‑LQGXFHGF\WRNLQHSURGXFWLRQLQK20)
Fumishige Oseko＊, Toshiro Yamamoto, Yoshiki Sato, Shigeta Takizawa,.HLML$GDFKL0LWXQREX$NDQH0DVDUX1LVKLJDNL1DULVDWR.DQDPXUD
Department of Dental Medicine, Kyoto Prefectural University of Medicine Graduate School of Medical Science
Background and objective: Beta‑FU\SWR[DQWKLQ（ȕ‑cry）, a carotenoid abundant in satsuma mandarin, has anticancer and
DQWLR[LGDQWH൵HFWV:HSUHYLRXVO\UHSRUWHGWKHDQWL‑LQÀDPPDWRU\H൵HFWVRIȕ‑FU\RQWKHSHULRGRQWDOOLJDPHQW+RZHYHUWKH
H൵HFWVRIȕ‑FU\RQRUDOPXFRVLWLVDQDGYHUVHHYHQWGXHWRFDQFHUFKHPRWKHUDS\UHPDLQXQFOHDU)OXRULQDWHGS\ULPLGLQHVDUHIUHTXHQWO\HPSOR\HGIRURUDOFDQFHUVZLWKDKLJKLQFLGHQFHRIVWRPDWLWLV,QWKHSUHVHQWVWXG\ZHH[DPLQHGWKHH൵HFWVRI‑ÀXRURXUDFLO（5‑FU）and ȕ‑FU\RQRUDOPXFRVDO¿EUREODVWV
Materials and Methods: +XPDQRUDOPXFRVDO¿EUREODVWV（hOMF）were cultured with 5FU（1 Hg/mL）and/or ȕ‑cry（100nM）7KHQDFHOOSUROLIHUDWLRQWHVWZDVSHUIRUPHGE\:67DVVD\DQGWKHH[SUHVVLRQVRILQIODPPDWRU\F\WRNLQHVDQG
apoptosis‑UHODWHGJHQHVZHUHH[DPLQHGE\UHDO‑WLPH57‑3&5
Results: The proliferation of hOMF was inhibited by the addition of 5‑FU, and the inhibition was suppressed with ȕ‑FU\,QÀDPPDWRU\F\WRNLQHVVXFKDV,/‑1ȕ,/‑DQG,/‑DQGWKHUDWLRRI%D[%FO‑2 in hOMF were increased with 5‑FU, andthe increase was suppressed with ȕ‑FU\Conclusion: Beta‑cry may have anti‑F\WRWR[LFDQGDQWL‑LQÀDPPDWRU\H൵HFWVRQRUDOPXFRVLWLVFDXVHGE\‑FU, suggesting
WKDWLWPD\SURYLGHDQRYHOPHWKRGIRUSUHYHQWLQJRUDOPXFRVLWLVGXHWRFDQFHUFKHPRWKHUDS\

JSP/JACP Poster Session AAP2020

General（Clinical Research）

19

GC‑01

&OLQLFDORXWFRPHVRIUK)*)‑WKHUDS\ZLWK0‑0,67IRULQWUDERQ\GHIHFWV.HQWDUR,PDPXUD＊-RRUHH,ZDNL.RXNL<RVKLNDZD+LGHWR$RNL7DNDKLUR%L]HQMLPD'DLVXNH,URNDZD)XPL6HVKLPD$WVXVKL6DLWR
Department of Periodontology, Tokyo Dental College
Background and objective: 0RGL¿HG0LQLPDOO\,QYDVLYH6XUJLFDO7HFKQLTXH（M‑0,67）（Cortellini and Tonetti 2009）
ZDVGHVLJQHGWRLPSURYHZRXQGVWDELOLW\DQGUHGXFHSDWLHQWPRUELGLW\7KLVFDVH‑series aimed to evaluate clinical outcomes
RISHULRGRQWDOUHJHQHUDWLYHWKHUDS\XVLQJUHFRPELQDQWKXPDQ¿EUREODVWJURZWKIDFWRU（rhFGF）‑2 with M‑0,67IRUWKH
WUHDWPHQWRILQWUDERQ\GHIHFWVMethods: )ROORZLQJLQLWLDOSHULRGRQWDOWKHUDS\VL[LQWUDERQ\GHIHFWV（mean depth；PPZLGWK；PP）in four
patients received rhFGF‑2 therapy with M‑0,67$ EXFFDO LQFLVLRQ ZDV SODFHG WR JDLQ DFFHVV WR WKH GHIHFW ZLWKRXWLQWHUGHQWDODQGOLQJXDOLQFLVLRQV$IWHUJUDQXODWLRQWLVVXHVZHUHUHPRYHGE\VKDUSGLVVHFWLRQDQGURRWLQVWUXPHQWDWLRQ％rhFGF‑ZDVDSSOLHGWRWKHGHIHFW)ODSVZHUHLPPHGLDWHO\FORVHGE\VXWXUHV3URELQJGHSWK（PD）, clinical attachmentlevel（CAL）, gingival margin（GM）, and radiographic bone fill were evaluated at baseline, 3 and 6 months
SRVWRSHUDWLYHO\Results: The mean surgical time was 48 ±PLQXWHV(DUO\ZRXQGKHDOLQJZDVXQHYHQWIXOSULPDU\ZRXQGFORVXUHVZHUHREWDLQHGLQDOOVLWHVDWZHHNSRVWRSHUDWLYHO\3DWLHQWVGLGQRWUHSRUWDQ\SDLQRUVZHOOLQJ$W‑months postoperatively,
PHDQ3'UHGXFWLRQZDV±PPDQG&$/JDLQZDV±PP$PLQLPDOLQFUHDVHLQJLQJLYDOUHFHVVLRQ（‑±PP）ZDVUHFRUGHG$QLPSURYHPHQWLQUDGLRJUDSKLFERQH¿OO（±％）ZDVQRWHG
Conclusion: This case series showed that rhFGF‑2 therapy with M‑0,67\LHOGHGIDYRUDEOHFOLQLFDODQGUDGLRJUDSKLF
RXWFRPHVLQWKHWUHDWPHQWRILQWUDERQ\GHIHFWVGC‑02

3HULRGRQWDOUHJHQHUDWLRQXVLQJUK)*)‑ZLWK'%%0$Q‑PRQWKIROORZ‑XSRIDUDQGRPL]HGFRQWUROOHGWULDO
Hideto Aoki＊1, 2, Atsushi Saito1, 2, Masahiro Sato1, Kouki Yoshikawa1:DWDUX<RVKLGD1,
Yurie Kitamura1, Daisuke Matsugami1, 2, Daichi Kita1, 2.HQWDUR,PDPXUD1, 2'DLVXNH,URNDZD3,7DNDKLUR%L]HQMLPD3, Akihito Soma1, Yasuo Hosaka1, Fumi Seshima1, Sachiyo Tomita3
1

Department of Periodontology, Tokyo Dental College, 2Oral Health Science Center, Tokyo Dental College,
3
Chiba Dental Center, Tokyo Dental College

Background and objective: ,QRXUHDUOLHUUDQGRPL]HGFRQWUROOHGWULDOFRPSDULQJXVHRIUHFRPELQDQWKXPDQ¿EUREODVWJURZWK
factor（rhFGF）‑2 in combination with deproteinized bovine bone mineral（DBBM）and rhFGF‑2 alone in the treatment
RILQWUDERQ\GHIHFWZHUHSRUWHGQRVLJQL¿FDQWGL൵HUHQFHLQFOLQLFDODWWDFKPHQWOHYHO（CAL）gain between groups at 6
PRQWKV:HDOVRVKRZHGWKDWWKHFRPELQDWLRQWKHUDS\\LHOGHGDQHQKDQFHGUDGLRJUDSKLFRXWFRPH7KHDLPRIWKLVIROORZ‑upVWXG\LVWRHYDOXDWHWKHRXWFRPHVDWPRQWKVSRVWRSHUDWLYHO\Materials and Methods: Participants in the base study（6DLWRHWDO-&3）were reevaluated at 12 and 18 months
SRVWRSHUDWLYHO\ 2ULJLQDO PHDVXUHV（clinical parameters and patient‑reported outcome measure）ZHUH UHSHDWHG
5DGLRJUDSKLFERQH¿OOZDVHYDOXDWHGDWPRQWKVResults: $WPRQWKVSRVWRSHUDWLYHO\DWRWDORIVLWHVLQSDWLHQWVZHUHUHHYDOXDWHG,QERWKWHVW（rhFGF‑2+DBBM）
and control（rhFGF‑2 only）JURXSVWKHUHZDVDSURJUHVVLYHLPSURYHPHQWLQ&$/DQG3'YDOXHVGXULQJPDLQWHQDQFHFDUH
1RVLJQLILFDQWGLIIHUHQFHEHWZHHQJURXSVZDVREVHUYHG$WPRQWKVPHDQYDOXHIRUUDGLRJUDSKLFERQHILOOLQWKHWHVW
group（％）was significantly greater than that in the control group（％）（S  ） ,Q ERWK JURXSV QR
VLJQL¿FDQWFKDQJHLQWRWDO2+54/VFRUHZDVREVHUYHGDWPRQWKVConclusion: ,QWKHWUHDWPHQWRILQWUDERQ\GHIHFWVXVLQJUK)*)‑2, with or without DBBM, further improvement in CAL wasobserved at 18‑month follow‑XS7KHFRPELQDWLRQWKHUDS\\LHOGHGDVLJQL¿FDQWO\JUHDWHUERQH¿OO,QERWKJURXSVIDYRUDEOH
FOLQLFDORXWFRPHVFDQEHVXVWDLQHGIRUDWOHDVWPRQWKVJSP/JACP Poster Session AAP2020

20

GC‑03

2UDOKHDOWKEHKDYLRUDQGWKHRUDOFRQGLWLRQRISUHJQDQWZRPHQ
1REXKLUR&KLQMX＊1, Michiko Furuta1, 20DVD\XNL1DNDQR1.RML2EDUD10DVDKLUR,NHGD1,,]XPL.RPLQDPL1, Takehiro Harada1, Megumi Oishi1, Yuko Koishi1, Satoru Haresaku1, 3,<XNLR,VKLNXUD1, Kazuyoshi Tanaka1, Keisuke Moritani1, Kimihiko Mishima1&\XNHQ5\X1,
Kiyotaka Hagiwara1<DVXIXPL7DMLPD1, Yuka Okabe1, 2, Shinya Yasumoto16KLQML.DQGD1
1

Fukuoka Dental Association, 2Section of Preventive and Public Health Dentistry, Division of Oral Health, Growthand Development, Faculty of Dental Science, Kyushu University, 3'HSDUWPHQWRI1XUVLQJ'LYLVLRQRI%DVLF
0HGLFDO6FLHQFHDQG)XQGDPHQWDO1XUVLQJ)XNXRND1XUVLQJ&ROOHJH

Background and objective: 7KHDVVRFLDWLRQEHWZHHQSHULRGRQWDOGLVHDVHDQGORZELUWKZHLJKWKDVEHHQSUHYLRXVO\UHSRUWHG
7KHUHIRUHPDLQWDLQLQJJRRGRUDOK\JLHQHGXULQJSUHJQDQF\LVLPSRUWDQWWRUHGXFHWKHULVNRIDSRRUSUHJQDQF\RXWFRPH
7KHREMHFWLYHRIWKLVVWXG\ZDVWRLQYHVWLJDWHRUDOKHDOWKEHKDYLRULQUHODWLRQWRRUDOFRQGLWLRQLQSUHJQDQWZRPHQ
Materials and Methods: )XNXRND&LW\SURYLGHVKHDOWKVHUYLFHVLQFOXGLQJGHQWDOH[DPLQDWLRQVIRUSUHJQDQWZRPHQ3UHJQDQWZRPHQ ZKR XQGHUZHQWH[DPLQDWLRQV EHWZHHQDQG（n = 4,275）SDUWLFLSDWHGLQ WKLVVWXG\7KH SUHVHQFHRI
GHFD\HGWHHWKZDVHYDOXDWHGDQGSHULRGRQWDOGLVHDVHZDVDVVHVVHGXVLQJWKHPRGL¿HG&RPPXQLW\3HULRGRQWDO,QGH[（&3,）
Results: The percentage of pregnant women with decayed teeth and deep periodontal pockets（SRFNHWVFRUHLQ&3,）was％DQG％ UHVSHFWLYHO\/RJLVWLFUHJUHVVLRQDQDO\VLVVKRZHGWKDWWKHSUHVHQFHRIGHFD\HGWHHWKZDVDVVRFLDWHGZLWKno regular dental visits before pregnancy（odds ratio［25］ ％FRQ¿GHQFHLQWHUYDO［&,］±）and eating
between meals, three times and over per day（25％&,±）7KHGHHSSHULRGRQWDOSRFNHWVZHUHDVVRFLDWHG
with no regular dental visits before pregnancy（25％&,±）DQGQRXVHRIGHQWDOÀRVV（25％&,±）Conclusion: This study found that pregnant women who did not regularly visit a dental clinic before pregnancy tended to
KDYHSRRURUDOFRQGLWLRQV,WLVVXJJHVWHGWKDWSUHJQDQWZRPHQIRFXVRQWKHLURUDOKHDOWKEHKDYLRULQRUGHUWRPDLQWDLQJRRGRUDOKHDOWK
GC‑04

6\VWHPLFSDUDPHWHUVDVVRFLDWHGZLWKSHULRGRQWDOLQÀDPHGVXUIDFHDUHD
7RVKL\D)XMLL＊11RULR$R\DPD1, Mariko Ozawa1, Sayuri Kida1,FKLURWD1R]DZD2,0RWRNL)XMLZDUD2, Katsushi Tamaki2, Masato Minabe1
1'HSDUWPHQWRI2UDO,QWHUGLVFLSOLQDU\0HGLFLQH.DQDJDZD'HQWDO8QLYHUVLW\2Department of Critical Care
Medicine and Dentistry, Kanagawa Dental University,

Background and objective: The association between periodontitis and systemic disease has been shown, and it has been
UHSRUWHGWKDWSDWLHQWVZLWKVHYHUHSHULRGRQWDOGLVHDVHDUHOLNHO\WRGHYHORSV\VWHPLFGLVHDVH3HULRGRQWDOLQÀDPHGVXUIDFH
area（3,6$）LV WKH WRWDO VXUIDFH DUHD RI WKH SHULRGRQWDO SRFNHW DW WKH VLWHV ZLWK EOHHGLQJ RQ SURELQJ$OWKRXJK WKHXVHIXOQHVVRI3,6$DVDSHULRGRQWDOLQGLFDWRULQIRUPDWLRQRQWKHOLQNEHWZHHQ3,6$DQGPDUNHUVIRUV\VWHPLFGLVHDVHLVVWLOOVFDUFH7KHSXUSRVHRIWKHSUHVHQWVWXG\ZDVWRFODULI\V\VWHPLFSDUDPHWHUVDVVRFLDWHGZLWK3,6$
Materials and Methods: 6XEMHFWVZHUHUHFUXLWHGDW0HGLFDODQG'HQWDO&ROODERUDWLRQ&HQWHULQ.DQDJDZD'HQWDO8QLYHUVLW\+RVSLWDOIURPWR3,6$ZDVFDOFXODWHGIURPWKHSHULRGRQWDOSRFNHWGHSWKDQGEOHHGLQJRQSURELQJDQGRUDOIXQFWLRQH[DPLQDWLRQVERG\FRPSRVLWLRQDQDO\VLVDQGEORRGWHVWVZHUHSHUIRUPHG&RPSDUDWLYHDQDO\VLVZDVSHUIRUPHGIRU
3,6$DQGPDUNHUVRIJHQHUDOFRQGLWLRQ7KH(WKLFV&RPPLWWHHRI.DQDJDZD'HQWDO8QLYHUVLW\DSSURYHGWKHSURWRFRORIWKH
present study（1R）
Results: Totally 200 participants（136 women and 64 men）ZLWKPHDQDJHRIZHUHHQUROOHGLQWKLVVWXG\7KHV\VWHPLF
PDUNHUV FRUUHODWHG ZLWK 3,6$ ZHUH ERG\ IDW PDVV SHUFHQW ERG\ IDW ERG\ PDVV LQGH[ ZDLVW FLUFXPIHUHQFH FDOI
circumference, and serum gamma‑*73,QDGGLWLRQRUDOPDUNHUVFRUUHODWHGZLWK3,6$ZHUHSURWHLQOHXNRF\WHKDOLWRVLV
salivary occult blood, and red‑FRPSOH[EDFWHULDLQGHQWDOSODTXH
Conclusion: 7KHUHVXOWVRIWKLVVWXG\UHYHDOHGDOLQNEHWZHHQ3,6$DSHULRGRQWDOLQÀDPPDWLRQPDUNHUDQGPDQ\V\VWHPLF
DQGRUDOGLVHDVHPDUNHUVJSP/JACP Poster Session AAP2020

21

GC‑05

5LVNIDFWRUVIRUFKHPRWKHUDS\‑LQGXFHGIHEULOHQHXWURSHQLDLQKHPDWRORJLFDOFDQFHUSDWLHQWVYuka Sogawa＊1, 2, Makoto Fukui16KLQJHQ1DNDPXUD3, Kimiko Sogabe3,
5\RKHL6XPLWDQL3, Masami Yoshioka2, Masahiro Abe3, Daisuke Hinode1
1

Department of Hygiene and Oral Health Science, Tokushima University Graduate School of Biomedical Sciences,
2
7RNXVKLPD%XQUL8QLYHUVLW\)DFXOW\RI+HDOWKDQG:HOIDUH3Department of Hematology, Endocrinology andMetabolism, Tokushima University Graduate School of Biomedical Sciences

Background and objective: Febrile neutropenia（)1）is one of the frequent adverse events of hematological cancer
FKHPRWKHUDS\7KHDLPRIWKLVVWXG\LVWRLQYHVWLJDWHWKHDVVRFLDWLRQEHWZHHQFKHPRWKHUDS\‑LQGXFHG)1DQGRUDOEDFWHULD
DQGRURUDOLPPXQLW\LQSDWLHQWVZLWKKHPDWRORJLFDOFDQFHU
Materials and Methods: Thirty‑WZRSDWLHQWVZLWKKHPDWRORJLFDOFDQFHUZKRUHFHLYHGWKHLU¿UVWFKHPRWKHUDS\DW7RNXVKLPD
8QLYHUVLW\+RVSLWDOZHUHHQUROOHGLQWKHVWXG\7KHFKHPRWKHUDSHXWLFGUXJXVHGWKHV\VWHPLFFRQGLWLRQWKHSHULRGLQFOXGLQJWKHSUHVHQFHRIIHYHUZHUHREWDLQHGIURPWKHSDWLHQWPHGLFDOUHFRUG6HFUHWRU\LPPXQRJOREXOLQ$（V,J$）in saliva and the
DQDHURELFEDFWHULDLQWRQJXHFRDWLQJRIHDFKVXEMHFWVZDVDVVHVVHGEHIRUHWKH¿UVWFKHPRWKHUDS\Results: (OHYHQ VXEMHFWV KDG DQ RQVHW RI )1（)1 VXEMHFWV）DQG  VXEMHFWV GLG QRW（non‑)1 VXEMHFWV）during the
REVHUYDWLRQSHULRGV$VLJQL¿FDQWGL൵HUHQFHZDVREVHUYHGLQWKHOHYHOVRIV,J$EHWZHHQWZRJURXSV（p＜）7KHUDWHRI
F. nucleatum count per total bacterial count（％）VKRZHGDKLJKHUWHQGHQF\LQWKH)1VXEMHFWVWKDQWKDWLQWKHQRQ‑)1
VXEMHFWV（S ）8VLQJ52&FXUYHDQDO\VLVWKHRSWLPDOFXW‑R൵SRLQWEDVHGRQWKH$8&LQWKHF. nucleatumV,J$UDWLRZDVDQGWKHULVNRI)1RQVHWZDVVLJQL¿FDQWO\KLJKHUDPRQJWKHJURXSRIF. nucleatumV,J$UDWLRFRPSDUHG
with the group of＜（+5S＜）Conclusion: These results suggest that the rate of F. nucleatumDQGWKHOHYHOVRIV,J$DWEDVHOLQHPLJKWEHUHODWHGWR)1
RQVHWDVULVNIDFWRUV

GC‑06(൶FDF\RIWRRWKEUXVKHVZLWKWLWDQLXPGLR[LGHHOHFWURGHDQGVRODUFHOO
Masaya Yoshimine＊, Hisahiro Kamoi'HSDUWPHQWRI'HQWDO&OLQLF1LSSRQ0HGLFDO6FKRRO&KLED+RNXVRK+RVSLWDO
Background and objective: (൵HFWLYHFRQWURORIGHQWDOSODTXHE\GDLO\EUXVKLQJLVFULWLFDOIRURUDOKHDOWK7RRWKEUXVKHVZLWKWLWDQLXPGLR[LGHHOHFWURGHDQGVRODUFHOOKDYHEHHQUHSRUWHGWREHH൶FLHQW；this study was designed to evaluate the dental
SODTXHUHPRYDOH൵HFWRIWKHVHWRRWKEUXVKHVMaterials and Methods: 7KHVXEMHFWVLQFOXGHGPHPEHUVRIWKH1LSSRQ0HGLFDO6FKRRO&KLED+RNXVRK+RVSLWDO'HQWDO&OLQLF ZKR KDG WKRURXJK NQRZOHGJH UHJDUGLQJ WKH WHFKQLTXHV RI PDLQWDLQLQJ RUDO K\JLHQH7KH VL[ WHHWK VHOHFWHG E\
5DPIMRUG（16, 21, 24, 36, 41, and 44）ZHUH XVHG7KH WRRWKEUXVKHV FRPSULVHG WKH VRQLF SRZHUHG W\SH（SOLADEY
5+<6+0®JURXS5）, the manual type with large solar cell（62/$'(<1®JURXS1）, and the manual type withsmall solar cell（SOLADEY 3®, group SO3）；DOOZHUHPDQXIDFWXUHGE\6KLNHQ&R/WG7KH5XVWRJL0RGLILHG1DY\
3ODTXH,QGH[ZDVXVHG
Results: 7KHUDWHRIGHQWDOSODTXHUHPRYDOLQWKHHQWLUHPRXWKZDV％IRUJURXS5％IRUJURXS1DQG％for group SO3；WKHVDPHWUHQGZDVQRWHGRQWKHVXUIDFHRIDOOVL[WHHWKConclusion: ,QWKLVVWXG\WRRWKEUXVKHVZLWKWLWDQLXPGLR[LGHHOHFWURGHDQGVRODUFHOOERWKVRQLF‑powered and manual
W\SHVZHUHH൵HFWLYHIRUSODTXHFRQWUROJSP/JACP Poster Session AAP2020

22

GC‑07

5HWURVSHFWLYHVWXG\RIGHQWDOLPSODQWWUHDWPHQWIRUSDWLHQWVZLWKSHULRGRQWLWLV
Mikiko Yamazaki＊1, Kosaku Yamazaki2, Keiso Takahashi2
1

Department of Endodontics and Periodontics , Ohu University, Graduate School of Dentistry, 2Division of
Periodontics, Department of Conservative Dentistry, Ohu University School of Dentistry

Background and objective: Although the choice of rehabilitation with implant‑VXSSRUWHG¿[HGSURVWKHVHVLQSHULRGRQWDO
therapy is increasing, the pathogenesis of peri‑LPSODQW GLVHDVH LQ WKH SDWLHQWV ZLWK SHULRGRQWLWLV LV VWLOO XQFOHDU:H
performed a retrospective study to assess the outcome of implant treatment for the patients with periodontitis and thepossible risk factors of peri‑LPSODQWGLVHDVH
Materials and Methods: Eighty seven patients had been treated comprehensively from 2006 to 2018 in Ohu University'HQWDO+RVSLWDODQGWKHQLPSODQWVZHUHHQUROOHGIRUWKLVUHWURVSHFWLYHVWXG\6XFFHVVUDWHVZHUHFDOFXODWHGIURPWKH
degree of marginal bone loss（MBL）DVSDUWRIDSURJQRVWLFVWXG\9DULRXVFOLQLFDOGDWDDQGSDWLHQWV‑related risk factors
VXFKDVVPRNLQJKDELWRFFOXVDOSDUDIXQFWLRQERQHDXJPHQWDWLRQDQGGLDEHWHVPHOOLWXVZHUHDQDO\]HG&KL‑square test was
XVHGIRUVWDWLVWLFDOSURFHVVLQJResults: 7KHDYHUDJHSHULRGDIWHUJHWWLQJRFFOXVDOIXQFWLRQZDV±PRQWKVDQGWKHVXUYLYDOUDWHZDV％
&RPSDUHGZLWKVXUYLYDOLPSODQWVWKHGLVLQWHJUDWHGLPSODQWKDGDVLQJOHLPSODQWDQGERQHDXJPHQWDWLRQZLWKDUWL¿FLDOERQH（*%5）FRPSDUHGZLWKVXUYLYDOLPSODQWV7KHVXFFHVVUDWHFDOFXODWHGE\0%/＜PPZDV％ , while MBL ≧ 3 mm
were found in the patients with high risk for periodontitis compared with others（p＜）Conclusion: 7KHVHUHVXOWVVXJJHVWWKDWSURVWKHVHVZLWKDVLQJOHLPSODQWDQG*%5FRXOGEHULVNIRUGLVLQWHJUDWLRQRILPSODQWand the progression of peri‑implantitis is much faster for high‑ULVNSDWLHQWVZLWKSHULRGRQWLWLVWKDQWKHRWKHUV

GC‑08&DURWLGDUWHU\FDOFL¿FDWLRQDQGSHULRGRQWDOGLVHDVHSURJUHVVLRQLQ-DSDQHVHSHRSOH
1DQDH'HZDNH＊1<DVXDNL,VKLRND1, Keiichi Uchida2, Akira Taguchi3,
Akihiro Yoshida4, 51REXR<RVKLQDUL 2, 4
1

Department of Operative Dentistry, Endodontology and Periodontology, School of Dentistry, Matsumoto Dental
University, 2Department of Collaborative oral health, Matsumoto Dental University Hospital, 3Department of Oral
DQG0D[LOORIDFLDO5DGLRORJ\6FKRRORI'HQWLVWU\0DWVXPRWR'HQWDO8QLYHUVLW\4Department Oral Health
Promotion, Graduate School of Oral Medicine, Matsumoto Dental University, 5Department of Oral Microbiology,
6FKRRORI'HQWLVWU\0DWVXPRWR'HQWDO8QLYHUVLW\Background and objective: The purpose of this study is to evaluate the association between alveolar bone loss（ABL）
GHWHFWHGRQSDQRUDPLFUDGLRJUDSKVDQGFDURWLGDUWHU\FDOFL¿FDWLRQ（CAC）
ZKLFKLVWKH¿QDOVWHSLQWKHGHYHORSPHQWRI
atherosclerosis, detected on Computed Tomography（CT）
Materials and Methods: 7KHVXEMHFWVZHUHSDWLHQWV（mean age ± 6'±\HDUV）who visited Matsumoto'HQWDO 8QLYHUVLW\ KRVSLWDO EHWZHHQ  DQG 7KH UDWH RI$%/ DQG QXPEHU RI SUHVHQW WHHWK ZHUH PHDVXUHG RQSDQRUDPLFUDGLRJUDSKV8QLYDULDWHDQDO\VHVZLWKWKHW‑test and the chi‑VTXDUHGWHVWZHUHXVHGWRHYDOXDWHWKHGL൵HUHQFHVLQ
DJHJHQGHUKLVWRU\RIGLVHDVHVQXPEHURISUHVHQWWHHWKDQG$%/EHWZHHQVXEMHFWVZLWKDQGZLWKRXW&$&0RUHRYHUPXOWLYDULDWHORJLVWLFUHJUHVVLRQDQDO\VLVZLWKIRUZDUGVHOHFWLRQZDVXVHG)LQDO\UHFHLYHURSHUDWLQJFKDUDFWHULVWLFFXUYH（52&）DQDO\VLVZDVXVHGWRFODULI\KRZGHJUHHIRUWKHLGHQWL¿FDWLRQRI&$&E\$%/
Results: 1XPEHU RI VXEMHFWV ZLWK DQG ZLWKRXW &$& ZDV  DQG  8QLYDULDWH DQDO\VHV VKRZHG WKDW &$& ZDVVLJQLILFDQWO\ DVVRFLDWHG ZLWK DJH K\SHUWHQVLRQ RVWHRSRURVLV QXPEHU RI SUHVHQW WHHWK DQG$%/ 0XOWLYDULDWH ORJLVWLF
UHJUHVVLRQDQDO\VLVZLWKIRUZDUGVHOHFWLRQDGMXVWHGIRUFRYDULDWHVUHYHDOHGWKDWWKHSUHVHQFHRI&$&ZDVVLJQLILFDQWO\associated with age（25  ％&, ‑）, hypertension（25  ％&, ‑）and ABL（25  ％ &, ‑）VLJQL¿FDQWO\,QWKH52&DQDO\VLVSUHGLFWLQJWKHSUHVHQFHRI&$&$852&ZDVWKH
KLJKHVW（95％ &, ‑）IRU$%/VLJQL¿FDQWO\Conclusion: ,WZDVVXJJHVWHGWKDWPHDVXUHPHQWRI$%/RQSDQRUDPLFUDGLRJUDSKVPD\EHDQH൵HFWLYHWRROIRULGHQWLI\LQJ
SDWLHQWVZLWKDQLQFUHDVHGULVNRI&$&

JSP/JACP Poster Session AAP2020

23

GC‑09

7KHLQÀXHQFHRISHULRGRQWDOGLVHDVHLQSHULSKHUDODUWHU\GLVHDVHSDWLHQWVKeitetsu, Kure＊11RULR$R\DPD27DNDQRUL,ZDWD1
1Department of Periodontology, Tokyo Medical and Dental University, 2Division of Periodontology, Kanagawa
Dental University

Background and objective: Peripheral artery disease（PAD）LVDNLQGRIDUWHULRVFOHURVLVWKDWOHDGVWRLVFKHPLDLQH[WUHPLW\
6RPHVWXGLHVUHSRUWHGWKDWSDWLHQWVZLWKSHULRGRQWLWLVDUHDWDULVNIRU3$'%XWHYLGHQFHRQWKHUHODWLRQVKLSEHWZHHQWKHVH
WZR GLVHDVHV KDV QRW \HW EHHQ FODULILHG FRPSOHWHO\7KHUHIRUH ZH SHUIRUPHG D FURVV‑sectional study to assess thisrelationship by comparing patients with PAD to those with arrhythmia（$55）DVDFRQWUROJURXS
Materials and Methods: A large‑scale survey was conducted on patients who received cardiovascular care at Tokyo Medical
DQG'HQWDO8QLYHUVLW\+RVSLWDO&OLQLFDOGHQWDO¿QGLQJVVXFKDV33'%23&$/DQGQXPEHURIWRRWKORVVZHUHPHDVXUHGLQ
DOOSDWLHQWV6XEJLQJLYDOSODTXHVDOLYDU\VDPSOHVSHULSKHUDOEORRGVDPSOHVZHUHDOVRFROOHFWHG%HVLGHVZHSHUIRUPHG
real‑WLPH3&5DQG(/,6$WRGHWHFWLQÀDPPDWRU\PHGLDWRUOHYHOVVXFKDVWXPRUQHFURVLVIDFWRU（71)）‑alpha, interleukin（,/）‑PDWUL[PHWDOORSURWHLQDVH（MMP）‑2, 8, 9 and bacterial counts and anti‑EDFWHULDODQWLERG\WLWHUVLQWKHVDPSOHV$OO
SDWLHQWVZLWK3$'DQGRQHWRRWKDWOHDVWZHUHH[WUDFWHGIURPWKHRYHUDOOVWXG\SRSXODWLRQ:HH[WUDFWHGSDWLHQWVZLWK$55
by matching the sample size of the group in terms of age, prevalence of hypertension, dyslipidemia, diabetes, obesity, andthe smoking rate（n=20 for each）

Results:7KHUHZHUHVLJQL¿FDQWO\KLJKHUYDOXHVLQ3$'SDWLHQWVWKDQ$55LQWHUPVRIVHUXPOHYHOVRI71)‑DOSKD,/‑6,
MMP‑DQGWRRWKORVV0XOWLYDULDWHORJLVWLFDQDO\VLVDOVRVKRZHGWKDWVLJQL¿FDQWO\KLJKHURGGVUDWLRVZHUHREVHUYHGLQWRRWKORVVDQG71)‑DOSKDRI3$'WKDQ$55
Conclusion: This study suggested that periodontal inflammation might be correlated with PAD through systemicLQÀDPPDWLRQGC‑10*HQHWLFDQG(QYLURQPHQWDO)DFWRUVRQ3HULRGRQWLWLVLQ(OGHUO\7ZLQV.XQLNR,NHJDPL＊1, Motozo Yamashita1.RML0LNL1$ULVD1LVKLNDZD2, Kouki Hashimoto2,
Tatsuya Morikawa21RERUX7DNHVKLWD2.D]XQRUL,NHEH3, Masahiro Kitamura2, Shinya Murakami2
1

Department of Periodontology & Endodontics, Osaka University Dental Hospital, 2Department of Periodontology,
Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, 3Department of
3URVWKRGRQWLFV*HURGRQWRORJ\DQG2UDO5HKDELOLWDWLRQ2VDND8QLYHUVLW\*UDGXDWH6FKRRORI'HQWLVWU\

Background and objective: 3HULRGRQWLWLVLVFDXVHGE\GHQWDOELR¿OPVZKLOHWKHJHQHWLFDQGHQYLURQPHQWDOIDFWRUVD൵HFWWKH
RQVHWDQGSURJUHVVLRQRIWKHGLVHDVHWKURXJKOLIH,QWKLVVWXG\ZHDLPHGWRHYDOXDWHWKHH൵RUWUDWLRVEHWZHHQJHQHWLFDQG
environmental factors on periodontitis by comparing the clinical phenotypes of periodontitis in pairs between monozygotic（0=）and dizygotic（'=）WZLQSDLUVLQHOGHUO\-DSDQHVHSRSXODWLRQV
Materials and Methods: SDLUVRIWZLQVZHUHH[DPLQHGDWWKH7ZLQ5HVHDUFK&HQWHURI2VDND8QLYHUVLW\*UDGXDWH
6FKRRORI0HGLFLQH7KHIROORZLQJWHVWVZHUHSHUIRUPHG）remaining teeth, 2）probing depth（PD）, 3）percentage of
bleeding on probing（BOP）sites（％）, 4）score of bone resorption, 5）Serum antibody titer for periodontopathic
EDFWHULDGHQWDOSODTXH
Results: Twin intra‑class correlations for PD and ％RI%23VLWHVLQ0=WZLQVZDVKLJKHUWKDQ'=WZLQV2QWKHRWKHUKDQG
WKRVHIRUWKHQXPEHURIUHPDLQLQJWHHWKDQGVFRUHRIERQHUHVRUSWLRQLQ0=WZLQVZDVFRPSDUDEOHWR'=WZLQV,Q0=
female twins, intra‑class correlations for ％ of BOP sites were high but for PD, score of bone resorption and number of
UHPDLQLQJWHHWKZHUHORZ
Conclusion: For the pathology of periodontitis, PD and BOP, which are related to the patientʼVLQÀDPPDWRU\UHVSRQVHVDUH
OLNHO\WREHUHODWLYHO\LQÀXHQFHGE\JHQHWLFIDFWRUVZKLOHERQHUHVRUSWLRQDQGWRRWKUHWHQWLRQDUHOLNHO\WREHUHODWLYHO\LQÀXHQFHGE\HQYLURQPHQWDOIDFWRUVLQHOGHUO\SRSXODWLRQVJSP/JACP Poster Session AAP2020

Case Report

25

CR‑01

&DVHUHSRUWV3HULRGRQWDOUHJHQHUDWLRQZLWK)*)‑SOXVȕ‑7&3
Shin‑ichi Takayama＊<XKHL+DVKLPRWR+LURIXPL.DQGD1DQD0DWVXQR6KLRUL<DPDPRWR
Takayama Dental Clinic
Background: +XPDQUHFRPELQDQW¿EUREODVWJURZWKIDFWRU（FGF‑2）was approved as a periodontal regenerative medicine
in Japan and has been commercially available（5HJURWK®）VLQFH6HYHUDOFOLQLFDOUHSRUWVGHPRQVWUDWHGWKDW)*)‑2
HYHQ DORQH FRXOG LQGXFH SHULRGRQWDO UHJHQHUDWLRQ HIILFLHQWO\ +RZHYHU LQ FDVHV ZKHUH ERQH GHIHFWV DUH ODUJH DQGcomplicated, complete recovery by FGF‑PLJKWQRWEHSRVVLEOH,QWKLVUHSRUWVXFFHVVIXOFDVHVWUHDWHGZLWK)*)‑2 plus
ȕ‑tricalcium phosphate（ȕ‑TCP）DVDERQH¿OOLQJPDWHULDOZLOOEHSUHVHQWHG
Case: Three patients（male 54‑year‑old, female 50‑year‑old, female 65‑year‑old）presented with severe one‑wall
infrabony defects at each anterior tooth site（#23, #12, #43, respectively）

Clinical Procedures and Outcomes: The periodontal regenerative operation with FGF‑2 plus ȕ‑TCP（Cerasorb® M granulat
dental, 150‑ 500 μm, Curasan, Germany）ZDVSHUIRUPHG7KHLQFLVLRQOLQHZDVDYRLGHGDERYHWKHERQHGHIHFWZKHUHYHUSRVVLEOH)XOOWKLFNQHVVEXFFDODQGOLQJXDOPXFRSHULRVWHDOÀDSVZHUHUHÀHFWHG5HVLGXDOFDOFXOXVDQGJUDQXODWLRQWLVVXHV
were thoroughly debrided and the root surfaces were treated with 24％ EDTA（Prefgel®, Straumann, USA）IRUPLQXWHV
Then, FGF‑2 plus ȕ‑7&3ZHUHDSSOLHGLQWRWKHLQIUDERQ\GHIHFWV%XFFDODQGOLQJXDOÀDSVZHUHFORVHGZLWKVLPSOHVXWXUHVXQGHUWHQVLRQOHVV3URIHVVLRQDOFOHDQLQJRIWKHVXUJLFDOVLWHVZDVSHUIRUPHGHYHU\YLVLWDQGSHULRGRQWDODQGGHQWDO;‑ray
H[DPLQDWLRQVZHUHFDUULHGRXWDIWHUWKHRSHUDWLRQFDUHIXOO\‑7 months after the operation, the new bone formation was
REVHUYHGDWWKHERQHGHIHFWVLQHDFKSDWLHQW
Conclusion: These case reports suggest that FGF‑2 plus ȕ‑TCP could be useful for periodontal regeneration in cases where
ERQHGHIHFWVDUHVHYHUH

CR‑02

7UHDWPHQWRIFKURQLFSHULRGRQWLWLVXVLQJUK)*)‑LQFRPELQDWLRQZLWK'%%07DNDKLUR%L]HQMLPD＊1'DLVXNH,URNDZD2, Yasuo Hosaka2, Junichi Takahashi2,
Sachiyo Tomita2, Atsushi Saito2
1

Tokyo Dental College Chiba Dental Center, Chiba, Japan, 2Department of Periodontology, Tokyo Dental College

Background: +HUH ZH UHSRUW D ‑year follow‑up of a chronic periodontitis patient who had received periodontal
regenerative therapy using rhFGF‑2 with deproteinized bovine bone mineral（DBBM）

Case: The patient was a 49‑year‑ROG ZRPDQ ZKR SUHVHQWHG ZLWK WKH FKLHI FRPSODLQW RI VZROOHQ JXPV$Q LQLWLDOH[DPLQDWLRQUHYHDOHGWKDW ％ of sites with a probing depth（PD）RI PPDQG ％ of sites with bleeding on
SURELQJ7KH2ʼ/HDU\SODTXHFRQWUROUHFRUGZDV％ 5DGLRJUDSKLFH[DPLQDWLRQUHYHDOHGDQJXODUERQHUHVRUSWLRQLQDQG$FOLQLFDOGLDJQRVLVRIJHQHUDOL]HGFKURQLFSHULRGRQWLWLV（Stage Ⅲ Grade B）ZDVPDGHClinical Procedures and Outcomes: The patient underwent initial periodontal therapy including plaque control, scaling and
URRWSODQLQJ$IWHU UHHYDOXDWLRQ SHULRGRQWDO UHJHQHUDWLYH WKHUDS\ XVLQJ UK)*)‑ ZHUH SHUIRUPHG RQ  DQG 
Combination therapy with rhFGF‑'%%0ZDVSHUIRUPHGRQ)ROORZLQJUHHYDOXDWLRQWKHSDWLHQWZDVSODFHGRQVXSSRUWLYHSHULRGRQWDOWKHUDS\$PPJDLQLQPHDQFOLQLFDODWWDFKPHQWOHYHO（CAL）was observed at the sites treatedwith rhFGF‑DW\HDUDIWHUVXUJHU\,QDQGUK)*)‑'%%0WUHDWPHQW\LHOGHG&$/JDLQVRIPPDQG
PPUHVSHFWLYHO\'LVWLQFWUDGLRJUDSKLFERQH¿OOZDVREVHUYHGDW\HDUIROORZLQJWKHWUHDWPHQWZLWKUK)*)‑'%%0Conclusion: ,QWKHSUHVHQWFDVHSHULRGRQWDOUHJHQHUDWLYHWKHUDS\UHVXOWHGLQDGHFUHDVHLQ3'DQGLPSURYHPHQWLQ&$/
Combination therapy using rhFGF‑'%%0\LHOGHGDQHQKDQFHGUDGLRJUDSKLFERQHILOO7KLVLPSURYHPHQWKDVEHHQDGHTXDWHO\PDLQWDLQHGRYHUD‑\HDUSHULRG

JSP/JACP Poster Session AAP2020

26

CR‑03

7ZR‑\HDUIROORZ‑XSFDVHRISHULRGRQWDOUHJHQHUDWLYHWKHUDS\XVLQJUK)*)‑
'DLVXNH,URNDZD＊17DNDKLUR%L]HQMLPD2, Kizuku Seida3, Sachiyo Tomita1, Atsushi Saito1,4
1

Department of Periodontology, Tokyo Dental College, 2Tokyo Dental College Chiba Dental Center, 3Seida Dental
Clinic, 4Oral Health Science Center, Tokyo Dental College

Background and objective: Here we report a two‑year follow‑up of a chronic periodontitis patient who received periodontal
regenerative therapy using rhFGF‑
Case: The patient was a 54‑year‑ROGPDQZKRUHTXHVWHGWUHDWPHQWRISHULRGRQWDOGLVHDVH,QLWLDOH[DPLQDWLRQUHYHDOHG
％ of sites with a probing depth（PD）RIPPPHDQ3'ZDVPP7KHOHYHORISODTXHFRQWURODVDVVHVVHGE\WKH
Oʼ/HDU\SODTXHFRQWUROUHFRUGZDV％5DGLRJUDSKLFH[DPLQDWLRQUHYHDOHGDQJXODUERQHUHVRUSWLRQLQDQG
Patientʼs oral health‑UHODWHG4R/ZDVDVVHVVHGE\WKH2+54/LQVWUXPHQW7KHWRWDO2+54/VFRUHLQWKLVSDWLHQWZDV$
clinical diagnosis of generalized chronic periodontitis（6WDJH,,,*UDGH%）ZDVPDGHClinical Procedures and Outcomes: The patient underwent initial periodontal therapy including plaque control, scaling and
URRWSODQLQJDQGURRWFDQDOWUHDWPHQW$IWHUUHHYDOXDWLRQDVHULHVRIVXUJLFDOLQWHUYHQWLRQVZHUHSHUIRUPHGLQ‑16, 26,
27, 33‑%HFDXVHWKHGLVWDODVSHFWRIDQGKDGLQWUDERQ\GHIHFWUHJHQHUDWLYHWKHUDS\XVLQJUK)*)‑2 was
SHUIRUPHG )ROORZLQJ UHHYDOXDWLRQ WKHQ WKH SDWLHQW ZDV SODFHG RQ VXSSRUWLYH SHULRGRQWDO WKHUDS\$W  \HDUV
postoperatively, in #14 and 33, the treatment yielded clinical attachment level（CAL）JDLQVRIPPDQGPPUHVSHFWLYHO\'LVWLQFWUDGLRJUDSKLFERQH¿OOZDVREVHUYHGIROORZLQJWKHWUHDWPHQWZLWKUK)*)‑6LWHVZLWKD3'RIPPLPSURYHGWR％DQGPHDQ3'WRPP7KHWRWDO2+54/VFRUHZDVLPSURYHGWR
Conclusion: At 2 years postoperatively, the regenerative therapy using rhFGF‑2 yielded improvements in clinical
SDUDPHWHUVUDGLRJUDSKLFERQH¿OODQGSDWLHQWʼs oral‑KHDOWKUHODWHG4R/

CR‑04

7UHDWPHQWRIFKURQLFSHULRGRQWLWLVXVLQJUK)*)‑DQG*75ZLWKERQHJUDIW
Kouki Yoshikawa＊, Atsushi Saito
Department of Periodontology, Tokyo Dental College
Background: Here we report a 1‑year follow‑up of a chronic periodontist patient who had received periodontal regenerative
therapy using recombinant human fibroblast growth factor（rhFGF）‑2 and guided tissue regeneration（*75）withDXWRJHQRXVERQHJUDIW
Case: The patient was a 71‑year‑old woman who presented with the chief complaint of mobile tooth（#14）$QLQLWLDOH[DPLQDWLRQUHYHDOHGWKDW％ of sites with a probing depth（PD）RI PPPHDQ3'ZDVPPDQG％ of sites
ZLWKEOHHGLQJRQSURELQJ7KH2ʼLeary plaque control record was 62％ 5DGLRJUDSKLFH[DPLQDWLRQUHYHDOHGDQJXODUERQH
resorption（#14, 15）
 )XUFDWLRQ LQYROYHPHQW ZDV'HJUHH Ⅱ IRU3DWLHQWʼs oral health‑related quality of life was
DVVHVVHG E\ WKH 2+54/ LQVWUXPHQW 7KH WRWDO 2+54/ VFRUH ZDV $ FOLQLFDO GLDJQRVLV RI JHQHUDOL]HG FKURQLF
periodontitis（Stage Ⅳ , Grade B）ZDVPDGHClinical Procedures and Outcomes: 7KH SDWLHQW XQGHUZHQW LQLWLDO SHULRGRQWDO WKHUDS\$IWHU UHHYDOXDWLRQ SHULRGRQWDO
regenerative therapy using rhFGF‑ZDVSHUIRUPHGRQDQG&RPELQDWLRQWKHUDS\ZLWK*75DQGDXWRJHQRXVERQH
JUDIWZDVSHUIRUPHGRQ)ROORZLQJUHHYDOXDWLRQWKHSDWLHQWZDVSODFHGRQVXSSRUWLYHSHULRGRQWDOWKHUDS\$PPJDLQ
in clinical attachment level（CAL）was recorded at the sites treated with rhFGF‑DW\HDUDIWHUVXUJHU\,Q*75 ＋
ERQHDOORJUDIWWUHDWPHQW\LHOGD&$/JDLQRIPP$QLPSURYHPHQWLQUDGLRJUDSKLFERQH¿OOZDVQRWHG2+54/VFRUHZDVUHGXFHGWR
Conclusion: ,QWKHSUHVHQWFDVHSHULRGRQWDOUHJHQHUDWLYHWKHUDS\UHVXOWHGLQDGHFUHDVHLQ3'DQGLPSURYHPHQWLQ&$/
UDGLRJUDSKLFERQH¿OODQG2+54/VFRUHJSP/JACP Poster Session AAP2020

27

CR‑05

7KH‑\HDUFRXUVHRIJHQHUDOL]HGFKURQLFSHULRGRQWLWLVLQDSDWLHQWZLWKSRVWHULRUELWHFROODSVH
Mitsutaka Funakoshi＊)XQDNRVKL'HQWDO2൶FH
Purpose: 3RVWHULRUELWHFROODSVHFDQFDXVHVHFRQGDU\RFFOXVDOWUDXPDDQGSURJUHVVLRQRISHULRGRQWLWLV,QWKHSUHVHQWFDVH
combined periodontal and partial orthodontic treatment improved the occlusion and periodontal environment for easier self‑care and enabled the patient to maintain a favorable condition for 27 years, despite not visiting a clinic for 12 years during
WKDWSHULRG
Case description: A 42‑year‑old female presented to the authorʼVFOLQLFRQ1RYHPEHUZLWKDFKLHIFRPSODLQWRIGLIILFXOW\ LQ FKHZLQJ ZLWK KHU EDFN WHHWK *HQHUDOL]HG JLQJLYDO UHGQHVV DQG VZHOOLQJ ZHUH REVHUYHG LQ DGGLWLRQ WR D
SHULRGRQWDOSRFNHWRIRYHUPP9HUWLFDOERQHGHIHFWZLWKDGHHSSHULRGRQWDOSRFNHWZDVREVHUYHG
Diagnosis: Generalized moderate chronic periodontitis with secondary occlusal trauma
Treatment protocol: The treatment protocol consisted of the following:（1）initial periodontal therapy,（2）re‑evaluation,（3）partial orthodontic treatment,（4）periodontal surgery,（5）re‑evaluation,（6）restorative dental treatment, and（7）supportive periodontal therapy（SPT）

Treatment course: Partial orthodontic treatment was performed in the mandible to straighten teeth 37 and 47 as well as
LPSURYHWKHSRVLWLRQRIHDFKPDQGLEXODUWRRWK)ODSVXUJHU\ZDVSHUIRUPHGRQWHHWKDQG$IWHUUHVWRUDWLYH
GHQWDOWUHDWPHQWVWDELOL]DWLRQRIWKHRFFOXVLRQDQGSHULRGRQWDOWLVVXHVZDVFRQ¿UPHGDQGWUHDWPHQWZDVWUDQVLWLRQHGWR637
Results and conclusions: The present case suggests the importance of creating proper occlusion and a favorable oral
environment that facilitates easy self‑FDUHIRUWKHSDWLHQW

CR‑06

7UHDWPHQWRIJHQHUDOL]HGFKURQLFSHULRGRQWLWLVZLWKUK)*)‑DQG*75-RRUHH,ZDNL＊1.HQWDUR,PDPXUD1, Kobun Tanaka2, Hiroki Sugito3,4 and Atsushi Saito1
1

Department of Periodontology, Tokyo Dental College, 2Tanaka Dental, 3Department of Operative Dentistry,
Cariology and Pulp Biology, Tokyo Dental College, 4Department of Dental Hygiene, Tokyo Dental Junior College
Background: )RUWKHWUHDWPHQWRIGHHSDQJXODUERQHGHIHFWVSHULRGRQWDOUHJHQHUDWLYHWKHUDS\LVLQGLFDWHG:HUHSRUWDFDVH
of generalized severe chronic periodontitis treated by periodontal regenerative therapy；recombinant human fibroblast
growth factor（rhFGF）‑2 and guided tissue regeneration（*75）
Case: The patient was a 54‑year‑ROGZRPDQZKRSUHVHQWHGZLWKWKHFKLHIFRPSODLQWRIVZHOOLQJLQPD[LOODU\ULJKWJLQJLYD
$QLQLWLDOH[DPLQDWLRQUHYHDOHGWKDW％RIVLWHVZLWKDSURELQJGHSWKRIPP7KHSUHYDOHQFHRIVLWHVZLWKEOHHGLQJ
RQSURELQJZDV％ 7KHSODTXHFRQWUROUHFRUGZDV％ 5DGLRJUDSKLFH[DPLQDWLRQUHYHDOHGYHUWLFDOERQHGHIHFWVDURXQGWKHPRODUV$FOLQLFDOGLDJQRVLVRIJHQHUDOL]HGFKURQLFSHULRGRQWLWLV（6WDJH,,,*UDGH&）ZDVPDGHClinical Procedures and Outcomes: 7KHSDWLHQWXQGHUZHQWLQLWLDOSHULRGRQWDOWKHUDS\$QLPSURYHPHQWZDVREVHUYHGLQSHULRGRQWDOFRQGLWLRQV DWUHHYDOXDWLRQ$ VHULHVRISHULRGRQWDOUHJHQHUDWLYHWKHUDS\ZLWKUK)*)‑2 were performed on
DQJXODUERQHGHIHFWVLQ*75ZDVSHUIRUPHGRQ2SHQÀDSGHEULGHPHQWZDVSHUIRUPHGRQ
)ROORZLQJUHHYDOXDWLRQWKHRUDOIXQFWLRQZDVUHVWRUHGXVLQJDOO‑ceramic crowns（#21, 26）7KHQWKHSDWLHQWZDVSODFHGRQVXSSRUWLYHSHULRGRQWDOWKHUDS\Conclusion: ,QWKHSUHVHQWFDVHRIJHQHUDOL]HGFKURQLFSHULRGRQWLWLVSHULRGRQWDOUHJHQHUDWLYHWKHUDS\ZLWKUK)*)‑2 and*75UHVXOWHGLQDVWDEOHSHULRGRQWDOFRQGLWLRQWKDWIDFLOLWDWHGIDYRUDEOHOHYHORISODTXHFRQWUROJSP/JACP Poster Session AAP2020

28

CR‑07

$&DVHRIUHJHQHUDWLYHWKHUDS\RIPD[LOODU\FDQLQHZLWKLQWUDERQ\GHIHFWIRUSHULRGRQWDOSURVWKHVLV:DWDUX$WVXWD＊, Yuka Atsuta%ORVVRPGHQWDOR൶FH
Background: ,QSDWLHQWVZLWKSHULRGRQWLWLVWKHRFFOXVDOORDGRQWKHDQWHULRUWHHWKLQFUHDVHVGXHWRWKHPLVVLQJPRODUWHHWKDORQJZLWKSURJUHVVLRQRIVKLIWLQJRIGLVHDVHGWHHWKDQGRFFOXVDOFROODSVHGXHWRVHFRQGDU\RFFOXVDOWUDXPD,QVXFKDFDVHWKHSURSULHW\RISUHVHUYLQJDVLQJOHWRRWKKDVDQRWDEOHH൵HFWRQWKHSURVWKHWLFGHVLJQ7KLVSDWLHQWXQGHUZHQWFRUUHFWLRQDQG
UHJHQHUDWLRQWKHUDS\IRUVHYHUHSHULRGRQWLWLVDQGLQWUDERQ\GHIHFWGXHWRRFFOXVDOWUDXPDLQDFDQLQH2FFOXVDOUHFRQVWUXFWLRQZDVSHUIRUPHGXVLQJSURVWKHVLVDQGJRRGSURJUHVVZDVDFKLHYHG
Case: The patient was a 58‑year‑ROGIHPDOHZKRZDV¿UVWH[DPLQHGLQ6HSWHPEHUIRUFKLHIFRPSODLQWRIVZHOOLQJLQUHJLRQDQGRUDOH[DPLQDWLRQUHYHDOHGJUDGH‑PRELOLW\DQGVZHOOLQJLQ$GHQWDOUDGLRJUDSKVKRZHGLQWUDERQ\
GHIHFWH[WHQGLQJXSWRUGRIWKHURRW+HUPHGLFDOKLVWRU\UHYHDOHGK\SHUWHQVLRQDQGDQRQ‑VPRNHU
Clinical procedure and outcome: An old bridge prosthesis using #23 as abutment teeth was removed, and after performing
SULPDU\SHULRGRQWDOWUHDWPHQWZDVH[WUXGHG:HWKHQSHUIRUPHGUHJHQHUDWLRQWKHUDS\XVLQJE‑FGF in order to restore
WKHSHULRGRQWDOWLVVXHRIWKDWVXSSRUWHGWKHPD[LOODU\SURVWKHVLV,WKDGDGRXEOHFURZQEULGJHVWUXFWXUHIRUUHPRYDEOH
GHVLJQGXHWRFRUUHVSRQGLQJIXWXUHZDVKRXWRIFHPHQWConclusion: %RQHIRUPDWLRQLQWKHDQWHULRUWRRWKDUHDRILPSURYHGDQGWKHWRRWKKDVUHPDLQHGVWDEOH:KHQGHVLJQLQJSURVWKHVLVWKDWXVHVSHULRGRQWDOO\D൵HFWHGWHHWKZLWKLQWUDERQ\GHIHFWVDVDEXWPHQWVWKHUHFRYHU\RIVXSSRUWLQJSHULRGRQWDOWLVVXHE\FRUUHFWLRQDQGUHJHQHUDWLRQWKHUDS\LVH[WUHPHO\XVHIXOIRUDFKLHYLQJVWDELOLW\CR‑08

2UWKRGRQWLFWUHDWPHQWLQWZRSDWLHQWVZLWKVHYHUHSHULRGRQWLWLVDQGKLJKSDWKRORJLFDOPRELOLW\RIWKHDQWHULRUWHHWK
6KRML,6+,7$1,＊,VKLWDQL'HQWDO2൶FH
Background: 3DWKRORJLFDOWRRWKPLJUDWLRQLVRIWHQREVHUYHGDVSHULRGRQWLWLVSURJUHVVHV+HUHZHUHSRUWWZRFDVHVRIVHYHUHSHULRGRQWLWLVZLWKVLJQL¿FDQWPRUELGWRRWKPLJUDWLRQIRUZKLFKH[WUDFWLRQZDVFRQVLGHUHGEXWZKHUHRUWKRGRQWLFWUHDWPHQW
VXFFHVVIXOO\OHGWRFRQVHUYDWLRQRIWKHWHHWK
Case 1: The patient was a 52‑year‑ROGZRPDQZKRZDVDVPRNHU7KHGDWHRILQLWLDOFRQVXOWZDV$SULO7KHFKLHIFRPSODLQWVZHUHJLQJLYDOVZHOOLQJDQGKHPRUUKDJH7KHSDWLHQW¿UVWZHQWWRDQRWKHUKRVSLWDODQGZDVUHFRPPHQGHGWRYLVLWDSHULRGRQWLVW7KHSDWLHQWKDGDPHGLFDOKLVWRU\RISHGLDWULFDVWKPDDQGVOLJKWK\SHUWHQVLRQ2UDOH[DPLQDWLRQLQGLFDWHG
JLQJLYDOUHGGHQLQJDQGVZHOOLQJDVZHOODVGHHSSHULRGRQWDOSRFNHWV2YHUELWHDQGRYHUMHWZHUHDOVRREVHUYHGKHUWRRWKZDVJUHDWO\GLVSODFHGLQWKHODELDOGLUHFWLRQDQGWKHPDQGLEXODUDQWHULRUWHHWKVKRZHGSURWUXVLRQDQGFURZGLQJ
Case 2: The patient was a 42‑year‑ROGZRPDQ+HUGHWDLOVDUHSULQWHGRQWKHSRVWHU
Details of Treatment and Results: ,Q ERWK FDVHV UH‑evaluation was performed after completion of basic periodontal
WUHDWPHQWDQGRUWKRGRQWLFWUHDWPHQWRUGH¿QLWLYHSHULRGRQWDOVXUJHU\ZDVSHUIRUPHG2UDOIXQFWLRQUHFRYHU\WKHUDS\ZDV
initiated, which was followed by supportive periodontal therapy（SPT）

Conclusion: %RWKFDVHVVKRZHGSDWKRORJLFDOWRRWKPLJUDWLRQWRWKHH[WHQWWKDWH[WUDFWLRQZDVFRQVLGHUHGEXWWKHXVHRI
orthodontic treatment and attempts to conserve the teeth as much as possible greatly contributed to patient motivation and
OHGWRWKHWHHWKEHLQJUHWDLQHG

JSP/JACP Poster Session AAP2020

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CR‑09

1HZVXUJLFDODSSURDFKIRUWKHWUHDWPHQWRISHULRGRQWDOLQWUDERQ\GHIHFWV<RVKLKLUR,ZDQR＊1,2, Satoka Shimizu1, Momo Hasegawa15\RNR.DJHQR11,ZDQR'HQWDO&OLQLF2'HSDUWPHQWRI3HULRGRQWRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWRU\

Background: ,QWKLVVWXG\ZHGHYLVHGDQHZVXUJLFDODSSURDFKIRUWKHWUHDWPHQWRISHULRGRQWDOLQWUDERQ\GHIHFWV
Cases: Case 1 was a 65‑year‑old male（non‑smoker）with target tooth 27 showing distal probing pocket depth（PPD）ofPPCase 2 was a 48‑year‑old female（non‑smoker）ZLWKWDUJHWWRRWKVKRZLQJGLVWDO33'RIPP
Treatment and Results: 3URFHGXUH$VFDOSHOEODGHZDVWLOWHGWRDSSUR[LPDWHO\°, and a full‑thickness incision to the
SHULRVWHXPZDVPDGHDWDQDQJOHRIDSSUR[LPDWHO\°from the distolingual line angle of the second molar while avoiding
WKHERQHGHIHFW$IWHUPDNLQJDQLQFLVLRQRQWKHJLQJLYDOVXOFXVRQWKHGLVWDO‑most surface of the second molar, a horizontal
incision was made in the bone defect from the lingual side to the buccal bone crest to form an interdental papilla‑like buccal
YDOYH$IWHU UHPRYDO RI LQIHFWHG JUDQXODWLRQ WLVVXH IROORZHG E\ GHEULGHPHQW DQG DSSOLFDWLRQ RI SHULRGRQWDO WLVVXHUHJHQHUDWLRQ PDWHULDO WKH ERQH JUDIW PDWHULDO ZDV SDFNHG$ QHHGOH ZDV LQVHUWHG LQWR WKH EXFFDO YDOYH DQG OLQJXDO
keratinized gingiva to perform internal cross‑mattress suturing, and the thread on the lingual side was used to perform
PRGLILHG PDWWUHVV VXWXULQJ 2QH WR WZR PRUH VLPSOH VXWXUHV ZHUH DSSOLHG DW WKH GLVWDO‑most end, and suturing was
FRPSOHWHG
,Q WKH UH‑evaluation performed 9 months postoperatively, dental computed tomography showed the presence of bone
IRUPDWLRQDQGDPHOLRUDWLRQRIWKH33'
Conclusion: This method is a minimally invasive procedure that allows for easy dissection, rapid completion of the
SURFHGXUHDQGVHFXUHZRXQGFORVXUH

CR‑10

‑\HDUIROORZ‑XSRIVHYHUHFKURQLFSHULRGRQWLWLVZLWKRFFOXVDOWUDXPD
Takamasa Ohyagi＊, Kiwa Ohyagi
Ohyagi dental clinic
Background: 6HYHUHFKURQLFSHULRGRQWLWLVFDXVHVWKHD൵HFWHGWRRWKORVHVDWWDFKPHQWDQGLQFUHDVHVPRELOLW\UHVXOWLQJLQRFFOXVDOWUDXPD:HUHSRUWRQDSHULRGRQWLWLVSDWLHQWZLWKRFFOXVDOWUDXPDRYHUDWHQ‑\HDUIROORZXS
Cases: A 58‑year‑old female presented on May , 2007 with the chief complaint of gingival swelling and pain in the
PD[LOODU\OHIWUHJLRQ％RIVLWHVKDGD33' PPDQG％RIVLWHVH[KLELWLQJ%237RRWKPRELOLW\〜 3 and PPD
PPLQWKHPRODUVZLWKRFFOXVDOLQWHUIHUHQFHDWWKHQRQ‑ZRUNLQJVLGHRQMDZPRYHPHQWVKDGD33'PPSDODWDOO\ZLWKPRELOLW\KDGD33'PPPHVLDOO\ZLWKPRELOLW\DQGDWZR‑ZDOOERQ\GHIHFWPHVLDOO\GHWHFWHG
Clinical Procedures and Outcomes: )XOOPRXWKGLVLQIHFWLRQZDVSHUIRUPHGZLWKD]LWKURP\FLQEHFDXVH3JLQJLYDOLVZDV
detected by the real‑WLPH 3&5（％）7KUHH PRQWKV ODWHU %23 LPSURYHG（8％）but the mobility in the molars
UHPDLQHG7KHUHIRUHDQGZHUHH[WUDFWHGDQGRFFOXVDODGMXVWPHQWZDVSHUIRUPHGRQWKHPRODUV$WWKHUHHYDOXDWLRQWRRWKPRELOLW\LPSURYHG5HJHQHUDWLYHWKHUDS\ZDVSHUIRUPHGRQDQGIRUWKHLQWUDERQ\GHIHFWVDQGVHYHQPRQWKVODWHUWKHUHJHQHUDWLRQZDVFRQ¿UPHGZDVVSOLQWHGWR
Conclusion: 3HULRGRQWDOWUHDWPHQWFRPELQHGZLWKRFFOXVDODGMXVWPHQWUHGXFHGWRRWKPRELOLW\LQGLFDWLQJWUDXPDIURPRFFOXVLRQZDVLQYROYHGZLWKSHULRGRQWLWLVSURJUHVVLRQ1RVLJQLILFDQWFKDQJHZDVREVHUYHGRYHUWHQ\HDUVDVLGHIURPHQDPHOGHIHFWRQZKLFKZDVWUHDWHGZLWKDFURZQUHVWRUDWLRQ637ZLOOFRQWLQXHZKLOHFRQWUROOLQJDQGHOLPLQDWLQJWUDXPDIDFWRUVDVZHOODVLQÀDPPDWLRQ

JSP/JACP Poster Session AAP2020

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CR‑11

/RQJWHUPREVHUYDWLRQKHDOLQJSDWWHUQDIWHUSHULRGRQWDOWUHDWPHQWV
Akira Makino＊
0$.,12'HQWDOR൶FH
Background: Long epithelial attachment（LEA）, which is previously known as an unstable healing condition, is considered
to be a healing pattern after scaling and root planing（653）DQGRSHQÀDSGHEULGHPHQW（OFD）
+RZHYHUDQDQLPDOVWXG\by Shimono et al［1］ demonstrated that LEA could be eventually replaced by connective tissue attachment（CTA）+HUH,UHSRUWWKH¿QGLQJVVKRZLQJWKHUHSODFHPHQWRI/($E\&7$LQDSDWLHQWZLWKVHYHUHSHULRGRQWLWLVZKRKDVXQGHUJRQH
periodontal maintenance（PM）IRU\HDUV［1］6KLPRQR03DWKRORJ\RI:RXQG+HDOLQJ1HZ(GLWLRQ±$QVZHULQJIURP%DVLF6FLHQFHWR&OLQLFDO4XHVWLRQV±,VKL\DNX3XEOLVKHUV7RN\R
Case Report: A 43‑year‑ROGZRPDQ¿UVWO\YLVLWHGRXUGHQWDOFOLQLFZLWKFKLHIFRPSODLQWRIJLQJLYDOVZHOOLQJDQGSDLQRIWRRWKRQ6HSWHPEHU)XOOPRXWKUDGLRJUDSKVLQGLFDWHGDOYHRODUERQHORVV（VWDJH,9JUDGH&SHULRGRQWLWLV）

Clinical Treatment and Outcomes: )ROORZLQJ 653 DQG RFFOXVDO DGMXVWPHQW 2)' ZDV FDUULHG RXW FRQVHTXHQWO\LQÀDPPDWLRQGLVDSSHDUHGDQGDVWDEOHFRQGLWLRQZDVUHWXUQHGLQWKHSHULRGRQWDOWLVVXHV'XULQJWKH30SHULRGWKHJLQJLYD
KDVEHFRPHWKLFNDQGHYHQWKHODPLQDGXUDKDVEHHQUDGLRJUDSKLFDOO\DSSDUHQW9ROXPHUHQGHUHG'&7LPDJHVKRZHGWKH
UHJHQHUDWLRQRIDOYHRODUERQHDIWHU\HDUVRIWKHGHEULGHPHQWVXJJHVWLQJWKDW/($LVUHSODFHGE\&7$
Conclusion: Long epithelial attachment is not an unstable periodontal healing pattern, but can be replaced by connective
WLVVXH&RQWLQXLQJPDLQWHQDQFHFDUHIRUHOLPLQDWLQJSHULRGRQWDOSRFNHWVDQGSUHYHQWLQJDUHFXUUHQFHRIIODUH‑up is of
H[WUHPHO\LPSRUWDQFH

CR‑12$FDVHRI5RRW&RYHUDJHIRUJLQJLYDOUHFHVVLRQVZLWK6XEHSLWKHOLDOFRQQHFWLYHWLVVXHDQGE)*)
Koshi Terai＊1&KXMR.LWDPXUD2.RLFKLUR)XMLED\DVKLñ+LUR\XNL7DNLQR⁴
1Takino Dental Clinic ,2.LWDPXUD'HQWDO&OLQLFñ)XMLED\DVKL'HQWDO&OLQLF⁴Takino Dental Clinic

Background: 7KHXVHRIVXEHSLWKHOLDOFRQQHFWLYHWLVVXHLQURRWFRYHUDJHHYLGHQWLDOO\SUHVHQWVSHUPDQHQWWUHDWPHQWUHVXOWV,WVFRPELQHGXVHZLWKWKHEDVLF¿EUREODVWJURZWKIDFWRU（bFGF）VHHPVPRUHH൵HFWLYHEHFDXVHLWSURPRWHVWKHJURZWKRISHULRGRQWDOOLJDPHQW¿EUREODVWVDQGWKHPLJUDWLRQDQGSUROLIHUDWLRQRIPXOWLSOHFHOOVIRUPLQJWKHSHULRGRQWDOWLVVXHHJYDVFXODUHQGRWKHOLDOFHOOVHSLWKHOLDOFHOOVDQGRVWHREODVWV:HSUHVHQWHGDFDVHZKHUHURRWFRYHUDJHXVLQJVXEHSLWKHOLDOFRQQHFWLYHWLVVXHDQGE)*)IDYRUDEO\WUHDWHGWKHJLQJL‑val
UHFHVVLRQRIWKHPD[LOODU\DQWHULRUWHHWK
Case: Patient: 51‑year‑old woman（non‑smoker）,QLWLDOYLVLW1RYHPEHU
Chief complaint: Cosmetic disturbance*HQHUDOPHGLFDOKLVWRU\1RQH'HQWDOKLVWRU\'HQWDOWUHDWPHQWDWDQRWKHUFOLQLF¿YH\HDUVSUHYLRXVO\([DPLQDWLRQILQGLQJV0LOGJLQJLYDOUHFHVVLRQRIDQGDQGPPJLQJLYDOUHFHVVLRQRI（&ODVV,XQGHU0LOOHU VFODVVL¿FDWLRQ）
Treatment and Results: 1）Basic periodontal treatment, 2）5H‑evaluation, 3）Periodontal surgery（root coverage）, 4）
5H‑evaluation, and 5）Maintenance
Periodontal surgical treatment:
23: Coronally‑DGYDQFHGÀDSXVLQJVXEHSLWKHOLDOFRQQHFWLYHWLVVXHDQG0RGL¿HGFRURQDOO\‑advanced tunneling technique using bFGF combined with subepithelial connective tissue
Three months postoperatively, follow‑XSZDVSHUIRUPHGRQFHDPRQWKDORQJZLWKUHJXODUPDLQWHQDQFH
&XUUHQWO\SHULRGRQWDOWLVVXHDWWKHWUDQVSODQWVLWHLVVWDEOHDQGWKHSRVWRSHUDWLYHFRXUVHLVIDYRUDEOH
Conclusion: 5HPDUNDEOHURRWFRYHUDJHZDVDFKLHYHGDWWKHJLQJLYDOUHFHVVLRQVLWHXVLQJE)*)DFHOO‑growth factor having
YDULRXVFHOOJURZWKDQGDQJLRJHQLFH൵HFWV7KXVEHWWHUUHVXOWVFDQEHREWDLQHGZLWKURRWFRYHUDJHWKDQZLWKFRQYHQWLRQDOPHWKRGV+RZHYHUFRQWLQXHGLQYHVWLJDWLRQVRQFRQWUROOLQJWKHGLVHDVHSURJUHVVLRQDUHQHFHVVDU\

JSP/JACP Poster Session AAP2020

31

CR‑13

3HULRGRQWDOUHJHQHUDWLYHWKHUDS\XVLQJUK)*)‑DQG'%%0IRUIXUFDWLRQGHIHFWVMasahiro Sato＊11DRNR:DWDQDEH2, Atsushi Saito1,3
1

Department of Periodontology, Tokyo Dental College, 2Tsukuba Dental Clinic, 3Oral Health Science Center, Tokyo
Dental College

Background: The key points in treatment of furcation involvements are to prevent further attachment loss and to makePDLQWHQDQFHHDVLHU+HUHZHUHSRUWDFKURQLFSHULRGRQWLWLVFDVHZLWKIXUFDWLRQLQYROYHPHQWVWUHDWHGE\UK)*)‑2 with
deproteinized bovine bone mineral（DBBM）

Case: The patient was a 50‑year‑ROGPDQZKRUHTXHVWHGWUHDWPHQWRISHULRGRQWDOGLVHDVH$QLQLWLDOH[DPLQDWLRQUHYHDOHG
WKDW ％ of sites with a probing depth（PD）RI  PP DQG ％ of sites with bleeding on probing（BOP）
5DGLRJUDSKLFH[DPLQDWLRQUHYHDOHGDQJXODUERQHUHVRUSWLRQLQDQG)XUFDWLRQLQYROYHPHQWV（Degree
Ⅱ）ZHUHREVHUYHGLQDQG$FOLQLFDOGLDJQRVLVRIJHQHUDOL]HGFKURQLFSHULRGRQWLWLV（Stage Ⅲ Grade B）was
PDGHClinical Procedures and Outcomes: 7KH SDWLHQW XQGHUZHQW LQLWLDO SHULRGRQWDO WKHUDS\$IWHU UHHYDOXDWLRQ SHULRGRQWDO
regenerative therapy using rhFGF‑2 were performed on #32 and combination therapy with rhFGF‑2 + DBBM was
SHUIRUPHGRQDQG)ROORZLQJUHHYDOXDWLRQRUDOIXQFWLRQZDVUHVWRUHGXVLQJD¿[HGEULGJHSURVWKHVLV$WWKH
WUDQVLWLRQWRVXSSRUWLYHSHULRGRQWDOWKHUDS\％RIVLWHVSUHVHQWHGZLWKD3'RIPPDQG％RIVLWHVZLWK%23$W
\HDUVSRVWRSHUDWLYHO\DPPJDLQLQFOLQLFDODWWDFKPHQWOHYHO（CAL）was observed in #32 treated with rhFGF‑
Furcation involvements in #26 and 46 treated with rhFGF‑'%%0\LHOGHG&$/JDLQRIPP
Conclusion: ,QWKHSUHVHQWFDVHRIFKURQLFSHULRGRQWLWLVZLWKIXUFDWLRQLQYROYHPHQWVWKHUHJHQHUDWLYHWKHUDSLHVUHVXOWHGLQD
GHFUHDVHLQ3'LPSURYHPHQWLQ&$/DQGIDYRUDEOHRVVHRXVDUFKLWHFWXUH

CR‑14,PSURYHPHQWRIXQHYHQFHUYLFDOOLQHDQGJXPP\VPLOHZLWKSHULRGRQWDOVXUJHU\$FDVHUHSRUW.RLFKLUR)XMLED\DVKL＊1, Tsuyoshi Kamiyama2&KXMR.LWDPXUD3,
Koshi Terai40LWVXKLUR,ZDWD5, Hiroyuki Takino6
1)XMLED\DVKL'HQWDO&OLQLF2Kamiyama Dental Clinic,3Kitamura Dental Clinic,4Takino Dental Clinic, 5Sakura Dental
Clinic, 6Takino Dental Clinic

Background: Bone level, gingival level, and continuity of the dentition are important for the long‑term stability of the
SHULRGRQWLXP7KHJLQJLYDOOHYHOFDQEHFRUUHFWHGE\SURVWKHWLFUHVWRUDWLRQ；however, periodontal surgery is required in
FRPSOLFDWHGFDVHV6HYHUDOFULWHULDLQÀXHQFHWKHHVWKHWLFJRDOVLQFOXGLQJWKHVPLOHOLQHFODVVL¿HGDVORZDYHUDJHRUKLJKThe gummy smile（＞PPRIJLQJLYDOH[SRVXUH）LVXVXDOO\FRQVLGHUHGXQHVWKHWLF2EWDLQLQJDQLGHDOJXPOLQHUHTXLUHV
DVVHVVPHQWRIELODWHUDOV\PPHWU\SDUDOOHOLVPJLQJLYDO]HQLWKSRLQWDQGLQWHUGHQWDOSDSLOODU\KHLJKW7KHIDFWRUVD൵HFWLQJthe gum line include bone morphology, bone level, tooth position and inclination, root shape, attached gingival width and
PRGHRIDWWDFKPHQWDQGELRW\SHRIWKHVHERQHOHYHOEDVHGRQWKHELRORJLFDOZLGWKLVWKHPRVWLPSRUWDQW
Case: A 26‑year‑ROGIHPDOHSDWLHQWSUHVHQWHGZLWKDFKLHIFRPSODLQWRIXQHVWKHWLFXSSHUIURQWWHHWK0HGLFDOKLVWRU\13
1RQVPRNHU
Treatment & Result: 7KLVFDVHZDVDVVHVVHGDVW\SHVXEJURXS%EDVHGRQWKHDOWHUHGSDVVLYHHUXSWLRQFODVVL¿FDWLRQE\&RVOHW3HULRGRQWDOVXUJHU\DQGSURVWKHWLFWKHUDS\ZHUHSHUIRUPHG
Conclusion: :HDFKLHYHGHVWKHWLFUHVWRUDWLRQSHULRGRQWDOVWDELOLW\DQGSDWLHQWVDWLVIDFWLRQLQWKLVFDVH,QDGGLWLRQWRSD\LQJ
attention to the shape and shade of the crowns for an aesthetic appearance during dental treatment, an optimal treatment plan
EDVHGRQH[DPLQDWLRQDQGGLDJQRVLVRIWKHUHODWLRQVKLSEHWZHHQWKHJXPVDQGWHHWKLHWKHKDUPRQ\RIWKHFHUYLFDOOLQH
PXVWEHIRUPXODWHG0RUHRYHUWKHKDUPRQ\EHWZHHQH[FHVVLYHJLQJLYDOGLVSOD\DQGWKHOLSVDQGWKHUHODWLRQVKLSZLWKWKH
VPLOHOLQHPXVWDOVREHFRQVLGHUHG

JSP/JACP Poster Session AAP2020

32

CR‑15

3HULRGRQWDOUHJHQHUDWLYHWKHUDS\ZLWK(0'DIRXU‑\HDUIROORZ‑XSFDVH
Fumi Seshima＊1, Yasugi Matsumoto2, Atsushi Saito1
1

Department of Periodontology, Tokyo Dental College, 2Matsumoto Dental Clinic

Background and objective: Here we report a four‑year follow‑up of a chronic periodontitis patient who received periodontal
UHJHQHUDWLYHWKHUDS\ZLWKHQDPHOPDWUL[GHULYDWLYH（EMD）

Case: The patient was a 50‑year‑ROGZRPDQZKRFRPSODLQHGRIPRELOHWHHWKLQWKHPRODUUHJLRQ,QLWLDOH[DPLQDWLRQUHYHDOHG ％ of sites with a probing depth（PD）RI PPIXOO‑PRXWKPHDQ3'ZDVPP7KH2ʼLeary plaque
FRQWUROUHFRUGZDV％ 5DGLRJUDSKLFH[DPLQDWLRQUHYHDOHGDQJXODUERQHUHVRUSWLRQLQ$
clinical diagnosis of generalized chronic periodontitis（6WDJH,,,*UDGH%）ZDVPDGHClinical Procedures and Outcomes: The patient underwent initial periodontal therapy including plaque control, scaling and
URRWSODQLQJ$IWHU UHHYDOXDWLRQ D VHULHV RI VXUJLFDOLQWHUYHQWLRQVZHUHSHUIRUPHGLQ‑
5HJHQHUDWLYHWKHUDS\XVLQJ(0'ZDVSHUIRUPHGRQWKHQDUURZLQWUDERQ\GHIHFWVRIDQGFRPELQDWLRQWKHUDS\XVLQJ(0'DQGDXWRJHQRXVERQHJUDIWZDVSHUIRUPHGRQWKHZLGHDQGGHHSLQWUDERQ\GHIHFWVRI
)ROORZLQJUHHYDOXDWLRQWKHQWKHSDWLHQWZDVSODFHGRQVXSSRUWLYHSHULRGRQWDOWKHUDS\$W\HDUVSRVWRSHUDWLYHO\
yielded clinical attachment level（CAL）gains of 4 ±PPDQG\LHOGHG&$/JDLQVRI ±
PPUHVSHFWLYHO\'LVWLQFWUDGLRJUDSKLFERQH¿OOZDVREVHUYHGIROORZLQJWKHWUHDWPHQWZLWK(0'6LWHVZLWKD3'RI
PPLPSURYHGWR％ and mean full‑PRXWK3'ZDVUHGXFHGWRPP
Conclusion: At 4 years postoperatively, the regenerative therapy using EMD yielded improvements in clinical parameters
DQGUDGLRJUDSKLFERQH¿OO

CR‑16*HQHUDOL]HG‑FKURQLFSHULRGRQWLWLVDQGW\SH‑GLDEHWHVZLWKFRPSUHKHQVLYHSHULRGRQWDOWKHUDS\
0DQD1DVX＊6DWRUX0RULNDZD7DQHDNL1DNDJDZD
Department of Dentistry and Oral Surgery, Keio University School of Medicine
Background: Chronic periodontitis and diabetes are closely linked, and it is known that controlling periodontitis improves
EORRGVXJDUOHYHO:HUHSRUWWKHFDVHRIDSDWLHQWZKRUHFHLYHGSHULRGRQWDODQGUHJHQHUDWLYHWKHUDS\6KHLPSURYHGZLWK
supportive periodontal therapy（SPT）DQGEORRGVXJDUFRQWURO
Case: A 67‑year‑old woman, who was diagnosed with type 2 diabetes mellitus and fatty liver, visited the hospital after
H[SHULHQFLQJDVWUDQJHIHHOLQJLQWRRWK$Q;‑UD\LPDJHUHYHDOHGDOHVLRQRQWKHDSH[RIWKHV\PSWRPDWLFWRRWK（tooth#9） *LQJLYDO VZHOOLQJ DQG GHHS SHULRGRQWDO SRFNHWV ZHUH REVHUYHG LQ WKH PRODUV GXH WR XQFRQWUROOHG GLDEHWHV 0LOG
KRUL]RQWDOERQHUHVRUSWLRQZDVREVHUYHGWKURXJKRXWWKHMDZHVSHFLDOO\LQWRRWKZKLFKZDVIXUFDWHG7HHWKDQG
had a cross‑ELWH
Clinical Procedures and Outcomes: ,QLWLDOSHULRGRQWDOWUHDWPHQWLQVWUXFWLRQRQRUDOK\JLHQHDQGWRRWKDSLFHFWRP\5H‑HYDOXDWLRQ3HULRGRQWDOVXUJHU\（WRRWKSHULRGRQWDOUHJHQHUDWLYHWKHUDS\XVLQJEDVLF¿EUREODVWJURZWKIDFWRUDQG
autologous bone）5H‑HYDOXDWLRQ)LQDOSURVWKHVLV637
Conclusion: 7KHSHULRGRQWDOSRFNHWVLPSURYHGDQGJLQJLYDOVZHOOLQJZDVQRWREVHUYHG7KHSDWLHQW VEORRGVXJDUFRQWUROLPSURYHG$OWKRXJKSRFNHWVZHUHREVHUYHGLQWKHPRODUVWKHWLVVXHZDVVWDEOHDQGEOHHGLQJRQSURELQJZDVQRWREVHUYHG
7RRWKZDVLQDJRRGFRQGLWLRQDIWHUDSLFHFWRP\DQGUHFXUUHQFHZDVQRWREVHUYHG1LJKWJXDUGVZHUHSUHVFULEHGIRUWKH
cross‑ELWHRQWKHOHIWVLGHRIWHHWKDQGWRUHGXFHDQRFFOXVDORYHUORDGZLWKWRRWK,QWHUYDOEHWZHHQ637FRQWLQXHG
IRUDIHZPRQWKVIRUFRQWURORISODTXHIRUPDWLRQ

JSP/JACP Poster Session AAP2020

33

CR‑17

5HVWRUH2UDOIXQFWLRQVZLWK3HULRGRQWDO7KHUDS\ZLWK*75
Yoko Koide＊, Matsuo Yamamoto
Department of Periodontology, Showa University School of Dentistry
Background: ,Q'HFWKHSDWLHQW（58‑year‑old male）KDGDFRQFHUQUHJDUGLQJKLVGLVFRPIRUWZKLOHFKHZLQJ7KLVZDVGXHWRWKHSURVWKHVLVEHLQJGHWDFKHGFDXVLQJGL൶FXOW\LQFKHZLQJE\WKHWHHWKDWWKHSRVWHULRUUHJLRQDQGWKHQDQWHULRUSHULRGRQWDOGHVWUXFWLRQ,QRUGHUWRWUHDWWKHPDVWLFDWRU\IXQFWLRQZHXVHGWKHSURVWKHWLFWUHDWPHQWDQGSHULRGRQWDODSSURDFKCase: *LQJLYDOVZHOOLQJDQGUHGQHVVZHUHREVHUYHGWKURXJKRXWWKHMDZ7KHSURELQJGHSWKPPZDV％ , bleeding on
probing was 31％&ODVV,IXUFDWLRQLQYROYHPHQWZDVREVHUYHGLQWRRWKDQG9HUWLFDOERQHUHVRUSWLRQZDVREVHUYHGLQWRRWKDQG6HYHUHERQHUHVRUSWLRQ（XSWRURRWDSH[）was observed at tooth #14, #22, and$FFRUGLQJWRWKHDERYH¿QGLQJVWKHSDWLHQWZDVGLDJQRVHGDV*HQHUDOL]HG6HYHUH&KURQLF3HULRGRQWLWLVZLWK2FFOXVDOTrauma（6WDJH,9DQG*UDGH%）
Clinical procedures and Outcomes: ,QLWLDOWKHUDS\ZDVSHUIRUPHGEHWZHHQ-DQXDU\DQG6HSWHPEHU7KLVZDVIROORZHG XS E\ SHULRGRQWDO VXUJHU\ ZKLFK LQYROYHG D *XLGHG7LVVXH 5HJHQHUDWLRQ ZLWK DEVRUEDEOH PHPEUDQH DQG DQ
DXWRJHQRXVERQHJUDIWDWWRRWKDQGDÀDSVXUJHU\DWWRRWKDQGXQWLO6HSWHPEHURI$IWHUWKHUH‑evaluation,
RUDOUHKDELOLWDWLRQZDVFRPSOHWHGZLWKWKHXVHRIDIL[HGDQGUHPRYDEOHSDUWLDOGHQWXUH0DLQWHQDQFHWKHUDS\KDVEHHQSHUIRUPHGVLQFH
Conclusion: This case report presented that recovering masticatory functions by using a therapeutic appliance and severe
LQWUDERQ\ GHIHFWV FDQ EH WUHDWHG ZLWK DEVRUEDEOH PHPEUDQH DQG DXWRJUDIWV7KLV PHWKRG LPSURYHG WKH SHULRGRQWDOSDUDPHWHUVDQGUDGLRJUDSKLFERQH¿OO

CR‑18

0DQDJHPHQWRIFDOFLXPFKDQQHOEORFNHU‑DVVRFLDWHGJLQJLYDORYHUJURZWKZLWKRXWFKDQJLQJPHGLFDWLRQSatoru Morikawa＊0DQD1DVX7DQHDNL1DNDJDZD
Department of Dentistry and Oral Surgery, Keio University School of Medicine
Background: Drug‑induced gingival overgrowth is a well‑NQRZQVLGHH൵HFWRIFDOFLXPFKDQQHOEORFNHUDGPLQLVWUDWLRQIRU
FDUGLRYDVFXODUFRQGLWLRQV$OWKRXJKJLQJLYDORYHUJURZWKLQGXFHVSHULRGRQWLWLVGHQWDOFDULHVDQGPDVWLFDWRU\G\VIXQFWLRQWKHUHFRPPHQGDWLRQWRGLVFRQWLQXHFDOFLXPFKDQQHOEORFNHUVLVFRQWURYHUVLDO
Case: :HUHSRUWDFOLQLFDOFDVHRIVHYHUHSHULRGRQWLWLVLQD‑year‑old Japanese male with a past medical history of
K\SHUWHQVLRQDQGW\SHGLDEHWHVPHOOLWXV+HH[KLELWHGJLQJLYDORYHUJURZWKZKLFKFRYHUHGWKHWHHWK+HFRQVXOWHGZLWKKLVSK\VLFLDQDERXWFKDQJLQJRUILQGLQJDQDOWHUQDWLYHIRUQLIHGLSLQHZKLFKZDVOLQNHGWRJLQJLYDORYHUJURZWK8OWLPDWHO\QLIHGLSLQHZDVGLVFRQWLQXHGDQGDPORGLSLQHZDVVWDUWHGGXULQJWKHFRXUVHRIKLVSHULRGRQWDOGLVHDVHWUHDWPHQWClinical Procedures and Outcomes: Periodontal treatment consisted of absolute oral hygiene instruction and scaling/root
SODQQLQJ%HFDXVHWKHSDWLHQWʼVEORRGSUHVVXUHZDVSRRUO\FRQWUROOHGQLIHGLSLQHZDVUHVXPHGGXULQJSHULRGRQWDOWUHDWPHQW+RZHYHUKLVSHULRGRQWDOFOLQLFDOVFRUHVZHUHVLJQL¿FDQWO\LPSURYHGZLWKRXWFKDQJLQJWRDQRWKHUFDOFLXPFKDQQHOEORFNHU
Conclusion: 5DWKHUWKDQEHLQJDGLUHFWH൵HFWRIFDOFLXPFKDQQHOEORFNHUDGPLQLVWUDWLRQDJJUHVVLYHJLQJLYDORYHUJURZWKPD\LQVWHDGUHVXOWIURPSRRURUDOK\JLHQH6LJQL¿FDQWLPSURYHPHQWRIJLQJLYDORYHUJURZWKZDVREVHUYHGIROORZLQJEDVLF
periodontal treatment, periodontal surgery, and intensive and regular follow‑ups to monitor oral hygiene without changing
WKHFDOFLXPFKDQQHOEORFNHUJSP/JACP Poster Session AAP2020

34

CR‑19

7HQ‑\HDUIROORZ‑XSRIDSDWLHQWZKRXQGHUZHQWFRPSUHKHQVLYHWUHDWPHQWIRUVHYHUHFKURQLF
SHULRGRQWLWLV
Takashi Takiguchi＊, Matsuo Yamamoto
Department of Periodontology Showa University School of Dentistry
Background and Objective: 6HYHUHFKURQLFSHULRGRQWLWLVLVDQLQÀDPPDWRU\GLVHDVHLQWKHSHULRGRQWLXPWKDWLVFKDUDFWHUL]HGE\SURJUHVVLYHGHVWUXFWLRQRIDWWDFKPHQWDQGERQHORVV:HUHSRUWWKH‑year follow‑up of a patient after periodontal
UHJHQHUDWLYHDQGORFDOL]HGRUWKRGRQWLFWKHUDSLHVIRUIXQFWLRQDOUHFRYHU\ZLWKSURVWKRGRQWLFLPSODQWV
Case: A 45‑year‑ROGZRPDQKDGDFKLHIFRPSODLQWRIWRRWKPRELOLW\DQGVZHOOLQJRIEDFNWHHWK6KHUHSRUWHGWKDWWKH
JLQJLYDKDGEHHQVZROOHQIRU\HDUV6KHZDVUHIHUUHGE\DJHQHUDOSUDFWLWLRQHUWRRXUGHQWDOKRVSLWDO,QWKHPRODUUHJLRQVLJQL¿FDQWWRRWKPRELOLW\DGHHSSHULRGRQWDOSRFNHW（8 mm）DQGLQIUDERQ\GHIHFWVZHUHREVHUYHG7KHGLDJQRVLVZDVJHQHUDOL]HGVHYHUHFKURQLFSHULRGRQWLWLV7UHDWPHQWSODQ；The following procedures were performed:（1）basic periodontal
therapy；（2）re‑H[DPLQDWLRQ；（3）periodontal surgery, including regeneration therapy；（4）re‑H[DPLQDWLRQ；（5）rehabilitation of oral function；（6）re‑H[DPLQDWLRQ；and（7）VXSSRUWLYHSHULRGRQWDOWKHUDS\Clinical Procedure and Outcomes: After basic periodontal therapy, we performed periodontal regenerative therapy and
ORFDOL]HGRUWKRGRQWLFWKHUDSLHV$¿QDOSURVWKHVLVZDVFKRVHQWRVHUYHDVDGHQWDOLPSODQWIRUHVWDEOLVKLQJVWDEOHRFFOXVLRQ
Consequently, the alveolar bone developed in a normal physiological form, and thorough cleansing of the oral cavity was
DFKLHYHGEHFDXVHRIWKHVKDOORZJLQJLYDOVXOFL7KHSDWLHQWʼs favorable condition has been maintained in the 10 years since
WKHWUHDWPHQW&RQFOXVLRQ&RPSUHKHQVLYHWUHDWPHQWFDQHQDEOHVWDEOHRFFOXVLRQDQGHVWDEOLVKPHQWRISHULRGRQWDOWLVVXHZLWK KLJK FOHDQVDEOLOW\ HYHQ LQ SDWLHQWV ZLWK VHYHUH SHULRGRQWLWLV$V D UHVXOW ORQJ‑term favorable prognosis can be
H[SHFWHG

CR‑20$EHUUDQWRFFOXVDOWUDXPDFDQFDXVHFHPHQWDOWHDUVWKDWLQGXFHSHULRGRQWLWLV
Kosaku Yamazaki＊1, Mikiko Yamazaki2, Keiso Takahashi1
1'LYLVLRQRI3HULRGRQWLFV'HSDUWPHQWRI&RQVHUYDWLYH'HQWLVWU\2KX8QLYHUVLW\6FKRRORI'HQWLVWU\2Department
RI(QGRGRQWLFVDQG3HULRGRQWLFV2KX8QLYHUVLW\*UDGXDWH6FKRRORI'HQWLVWU\Background: 0XOWLSOHULVNIDFWRUVDUHLQYROYHGLQWKHSURJUHVVLRQRISHULRGRQWDOGLVHDVH(VSHFLDOO\ORFDOIDFWRUVVXFKDV
anatomic anomaly, occlusal trauma and inadequate dental arch may induce a site‑VSHFLILFH[DFHUEDWLRQRISHULRGRQWDOWLVVXHV &HPHQWDO WHDUV（CTs）has been sometimes found when periodontal surgery has been performed where acute
JLQJLYDOLQÀDPPDWLRQDQGWLVVXHGHVWUXFWLRQKDGEHHQRFFXUUHGSUHYLRXVO\:HVKRZWKHFDVHVHULHVRIVXEFOLDJQRVWLF&7V
DQGGLVFXVVWKHSRVVLELOLW\WKDWRFFOXVDOWUDXPDPD\LQGXFHWKH&7VDQGSOD\DUROHRQWKHSURJUHVVLRQRISHULRGRQWLWLV
Cases: Three patients, 55, 70 and 71‑year‑ROGZHUHHQUROOHG:HREVHUYHGDOORIWKHPKDGDKDELWXDOEUX[LVP&OLQLFDO¿QGLQJVZHUHUHFRUGHGEHIRUHDQGDIWHUWUHDWPHQW&7VZHUHQRWIRXQGIURPRUDOUDGLRJUDSKVDWWKHLU¿UVWYLVLWEXW¿UVWO\IRXQG GXULQJ SHULRGRQWDO VXUJHU\ 'XULQJ SHULRGRQWDO VXUJHU\ UHJHQHUDWLYH SHULRGRQWDO WKHUDS\ ZLWK (PGRJDLQ® was
SHUIRUPHGDIWHUPHWLFXORXVURRWGHEULGHPHQW$IWHUWKHWUHDWPHQWFOLQLFDOLQGLFHVZHUHLPSURYHG7KHSURELQJSRFNHWGHSWK
decreased to less than 3 mm without BOP and the radiolucency was improved and the lamina dura was observed at all theVLWHV,QDOOFDVHVLWZDVVWDEOHIRUDWOHDVW\HDUVXQGHUWKHFRQWURORIRFFOXVDOG\VIXQFWLRQ
Conclusion: $EHUUDQWRFFOXVDOWUDXPDFDQFDXVH&7VDQGWKHQLQGXFHWKHUDSLGGHVWUXFWLRQRISHULRGRQWDOWLVVXHV7UHDWPHQWUHJLPHQE\ERWKUHJHQHUDWLYHSHULRGRQWDOWKHUDS\DQGWKHUHJXODWLRQRIRFFOXVDOWUDXPDPD\EHH൵HFWLYHJSP/JACP Poster Session AAP2020

35

CR‑21

1RQ‑VXUJLFDOWUHDWPHQWDQGPLFURELRPHRISHULRGRQWLWLVDQGSHUL‑LPSODQWLWLVVHYHQ‑\HDUIROORZ‑XS
.HLML.RPDWVX＊1, Takahiko Shiba17DND\DVX:DWDQDEH27DNDVKL1HPRWR1,
Tatsuro Koyanagi1, Yasuo Takeuchi17DNDQRUL,ZDWD1
1Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental
University, 2'HSDUWPHQWRI&KHPLVWU\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWU\

Background and objective: 1RQ‑surgical therapy is a standard method for periodontitis and has been reported to beineffective for peri‑LPSODQWLWLV +HUH ZH UHSRUW D FDVH RI VXFFHVVIXO QRQ‑surgical treatment for peri‑implantitis andperiodontitis with long‑WHUPVWDELOLW\
Case: The patient was a 69‑year‑ROGPDQZLWKFKLHIFRPSODLQWVRIGLVFRPIRUWLQULJKWPD[LOODLPSODQWVKRZHGSURELQJ
depth（PD）8mm with bleeding on probing（BOP） DQG  WHHWK VKRZHG 3' PP ZLWK %23 %RQH ORVV ZDVUDGLRJUDSKLFDOO\REVHUYHGDURXQGERWKGLVHDVHGVLWHV2 /HDU\SODTXHFRQWUROUHFRUG（3&5）ZDV％DWWKH¿UVWYLVLWClinical Procedures and Outcomes: An ultrasonic instrument and gracey curettes were used for decontamination of #17 andDIWHUWKH3&5UHDFKHGDOHYHORI％ $WWKH‑year follow‑up, no periodontal problems were observed in both sites
DQGJRRGRUDOK\JLHQHZDVPDLQWDLQHG0LFURELDODQDO\VLVEDVHGRQ6U'1$VHTXHQFLQJZDVFRQGXFWHGEHIRUHDQGDIWHUWUHDWPHQW3HULRGRQWDOSDWKRJHQVLQFOXGLQJPorphyromonas gingivalis were predominant at #17 and #34 before treatment,
whereas they were decreased at both sites after the follow‑XS7KHEDFWHULDOFRPSRVLWLRQZLWKLQWKHPLFURELRWDDIWHUWKH
follow‑XSZDVSUHGLFWHGWREHVLPLODUWRWKDWRIWKHKHDOWK\VLWH
Conclusion: This case report demonstrated clinically and bacteriologically that non‑VXUJLFDOWUHDWPHQWFDQEHH൵HFWLYHLQ
peri‑LPSODQWLWLV,PSURYHPHQWRIVHOI‑performed plaque control and low‑amount horizontal bone loss would be key points
IRUWKHVXFFHVVRIWUHDWPHQW3HULRGLFLQVSHFWLRQRIWKHEDFWHULDOFRPSRVLWLRQZLWKLQWKHSHUL‑implant microbiota may be
XVHIXOLQFOLQLFDOSUDFWLFH

CR‑22$FDVHUHSRUWRIVHYHUHFKURQLFSHULRGRQWLWLVZLWKGLDEHWHV
Tatsuo Hirano＊1, Takamasa Ohyagi21DRPDVD<DVXGD3, Yuichiro Togashi4,
Kento Murao1, Tetsuaki Hirano1'DLML<DPDJXFKL1, Jiro Hirano1
1

Hirano dental clinic, 2Ohyagi dental clinic, 3Carna dental clinic, 4Togashi dental clinic

Background: Patients with diabetes mellitus（DM）and severe chronic periodontitis need not only a medical but a dental
DSSURDFK,QVXFKSDWLHQWVVXUJLFDODSSURDFKLVUHTXLUHGDIWHUHYDOXDWLQJLQLWLDOSUHSDUDWLRQDQGFRQWUROOLQJ'0:HUHSRUWDVHYHUHFKURQLFSHULRGRQWLWLVSDWLHQWZLWK'0
Case: A 46‑year‑ROGPDOHSUHVHQWHGRQ-XO\ZLWKWKHFKLHIFRPSODLQWRIWRRWKPRELOLW\LQWKHPDQGLEXODUOHIWUHJLRQ
+HKDGEHHQWDNLQJDQWLK\SHUWHQVLYHDQGDQWLGLDEHWLFGUXJV+LV%3DQG+E$FZHUHDQG％ UHVSHFWLYHO\$QWHULRU
guidance was lost, 46％ of sites had PPD ≧4mm and 65％RIVLWHVH[KLELWHG%230RODUVVKRZHGWRRWKPRELOLW\WRsites with PPD ≧PPDQGIXUFDWLRQLQYROYHPHQWVKDGDSDODWDOPP33'ZLWKPRELOLW\DQGDPHVLDOWR‑wall
ERQ\GHIHFWKDGDPHVLDOPP33'ZLWKPRELOLW\DQGDPHVLDOWR‑ZDOOERQ\GHIHFWClinical Procedures and Outcomes: 8 months after initial preparation, BOP and PPD ≧4mm improved to 18％ and 30％ ,UHVSHFWLYHO\653ZLWKDQHVWKHVLDDQGRFFOXVDODGMXVWPHQWZDVVXEVHTXHQWO\SHUIRUPHGIROORZHGE\UHVROXWLRQRIWKH
PRELOLW\LQWKHPRODUV5HJHQHUDWLYHWKHUDS\ZDVSHUIRUPHGLQWKHLQWUD‑ERQ\GHIHFWVRIDQGDQGZHUH
WUHDWHGE\D¿[HGSDUWLDOGHQWXUH
Conclusion: ,QLWLDOSUHSDUDWLRQFRPELQHGZLWK653DQGRFFOXVDODGMXVWPHQWLPSURYHGURRWIXUFDWLRQLQYROYHPHQWVUHGXFHGWRRWKPRELOLW\DQGH[KLELWHGUHPLVVLRQRIGLDEHWHV7KHVHZHUHFRQVLGHUHGWREHGXHWRWKHUHGXFWLRQRILQÀDPPDWLRQE\SHULRGRQWDOWUHDWPHQW637ZLOOFRQWLQXHZKLOHFRQWUROOLQJDQGHOLPLQDWLQJWUDXPDIDFWRUVDVZHOODVLQÀDPPDWLRQ

JSP/JACP Poster Session AAP2020

36

CR‑23

/RQJ‑WHUPFRPSUHKHQVLYHPDQDJHPHQWRIJHQHUDOL]HGVHYHUHFKURQLFSHULRGRQWLWLV$FDVHUHSRUW
0D\XPL1DND\DPD＊
Ukita Dental Clinic
Background: 6HYHUH FKURQLF SHULRGRQWLWLV FDQ FDXVH SDWKRORJLFDO WRRWK PLJUDWLRQ RU PDODOLJQPHQW7KXV PDLQWDLQLQJDSSURSULDWHDQGVWDEOHWRRWKDOLJQPHQWLVHVVHQWLDOLQWKHPDQDJHPHQWRIVHYHUHFKURQLFSHULRGRQWLWLV7KLVFDVHUHSRUWpresents the failure, recovery, and practical consideration in the 10‑year long‑term management of tooth alignment in a
SDWLHQWZLWKVHYHUHFKURQLFSHULRGRQWLWLV
Case: A 63‑year‑ROGIHPDOH¿UVWYLVLWHGRQ)HEUXDU\ZLWKVHYHUHSHULRGRQWDOGLVHDVHDVVRFLDWHGZLWKFURZGLQJRIDQWHULRUWHHWKDQGRFFOXVDOWUDXPDClinical Procedures and Outcomes: )LUVWLQLWLDOSHULRGRQWDOWUHDWPHQWZDVLQLWLDWHG$IWHUUHHYDOXDWLRQSHULRGRQWDOVXUJLFDO
treatment with Emdogain®DQGDXWRORJRXVERQHJUDIWLQJZHUHSHUIRUPHG$IWHUPRQWKVZHSHUIRUPHGSDUWLDORUWKRGRQWLFWUHDWPHQW)LQDOO\SHULRGRQWDOSURVWKHVHVZHUHSHUIRUPHGRQWKHXSSHUDQGORZHUPRODUV%RWKWKHXSSHUDQGORZHUDQWHULRUWHHWKUHPDLQHG¿[HGIRUDZKLOHDQGZHUHSODFHGLQ637$IWHU\HDUVDQGPRQWKVÀDUH‑ups occurred at 12, 11 Out；thus,
we used 12, 11, 21, and 23 abutment teeth and a 22‑SRQWLFEULGJHWRUHSODFHWKHP&XUUHQWO\DGHFDGHKDVSDVVHGVLQFHZH
began this treatment, and the patientʼVVHYHUHFKURQLFSHULRGRQWLWLVKDVEHHQPDQDJHGZHOO
Conclusion: 7KHWRRWKSRVLWLRQLQWKHVSOLQWZLWKUHVLQDQGRFFOXVDODGMXVWPHQWPXVWEHMXGLFLRXVO\FRQVLGHUHGWRPDLQWDLQVWDEOHGHQWLWLRQDVDWHPSRUDU\VSOLQWQHHGVUHSDLUEHFDXVHRIWKHFRPSRVLWHUHVLQGHWHULRUDWLRQ+HQFH¿[HGSURVWKHVLVFRXOGEHSUHIHUUHGRYHUWHPSRUDU\VSOLQWWRPDLQWDLQVWDEOHGHQWLWLRQLQWKHPDQDJHPHQWRIFKURQLFSHULRGRQWLWLVCR‑24

2QH‑VWDJHIXOOPRXWKD‑3'7LQVHYHUHFKURQLFSHULRGRQWLWLV$FDVHUHSRUW<DVXKLUR1DND\DPD＊
1DND\DPD'HQWDO&OLQLF
Background and objective: Antimicrobial photodynamic therapy（a‑PDT）has a potential to be an effective treatment
DGMXQFWLQQRQ‑VXUJLFDOWUHDWPHQWRIFKURQLFSHULRGRQWLWLV2QH‑stage full mouth disinfection（Os‑FMD）protocols have
EHHQSURYHQWREHDUHOHYDQWWUHDWPHQWRSWLRQ7KLVFDVHUHSRUWVKRZV2V‑FMD combination with a‑PDT in treatment of
VHYHUHFKURQLFSHULRGRQWLWLVSDWLHQW
Case: A 51‑year‑ROGPDOHYLVLWHGP\FOLQLFLQ)HEUXDU\ZLWKWKHFKLHIFRPSODLQWRIRFFOXVDOSDLQ3HULRGRQWDOSRFNHWGHSWKZDVWRPP7KHSHUFHQWDJHRIVLWHVZLWK33' ≧PPZDV％ 7KHSHUFHQWDJHRI%23ZDV％ 0RELOLW\RIPRVWPRODUVZDVVHFRQGRUWKLUGGHJUHH7KHSDWLHQWZDVGLDJQRVHGDVJHQHUDOL]HGVHYHUHSHULRGRQWLWLV
Clinical Procedures and Outcomes: Before one‑stage full mouth a‑3'7DQGZHUHH[WUDFWHG$IWHUPHWLFXORXV
supragingival scaling, the a‑3'7ZLWKVXEJLQJLYDO653ZDVDSSOLHGLQRQHVHVVLRQDVDRQH‑stage full‑mouth a‑PDT on all
SHULRGRQWDOVLWHVD‑PDT involves toluidine blue as photosensitizer and diode laser（632‑644 nm）DVOLJKWVRXUFH
7KHUHVXOWZDVVDWLVIDFWRU\7KHSHUFHQWDJHRIVLWHVZLWK33' ≧PPGHFUHDVHGWR％ 7KHSHUFHQWDJHRI%23GHFUHDVHG
to 0％ $VLJQL¿FDQWLPSURYHPHQWRISHULRGRQWDOFRQGLWLRQZDVDFKLHYHG
Conclusion: The result of this treatment show that Os‑FMD combination with a‑3'7LVDQXVHIXODGMXQFWLQQRQ‑surgical
WUHDWPHQWWRLPSURYHSHULRGRQWDOFRQGLWLRQDQGWRVKRUWHQWUHDWPHQWSHULRGLQFKURQLFSHULRGRQWLWLVJSP/JACP Poster Session AAP2020

Dental Hygiene

37

DH‑01

7KHG\QDPLFUHVSRQVHVGXULQJPDLQWHQDQFHSKDVHDIWHUWKHUHJHQHUDWLYHVXUJHU\E\)*)‑
1DQD0DWVXQR＊, Shin‑ichi Takayama
Takayama Dental Clinic
Background: The efficacy of human recombinant fibroblast growth factor（FGF‑2）medicine（5HJURWK®）has been
GHPRQVWUDWHG E\ PDQ\ FOLQLFDO FDVHV LQ -DSDQ +RZHYHU WKHUH LV QR UHSRUW RQ KRZ SHULRGRQWDO WLVVXH UHJHQHUDWHG E\
5HJURWK®FKDQJHVRYHUDORQJSHULRGRIWLPH:HZLOOUHSRUWDFDVHLQZKLFKWKHVRFLDODQGHQYLURQPHQWDOLPSDFWRQWKH
SDWLHQWZDVREVHUYHGPDUNHGO\GXULQJWKHPDLQWHQDQFHSKDVHIROORZLQJWKHSHULRGRQWDOUHJHQHUDWLYHVXUJHU\
Case: A 42‑year‑old female with a one‑ZDOOHGVHYHUHLQIUDERQ\GHIHFWDQG&ODVV,,,IXUFDWLRQLQYROYHPHQWDWWRRWK
DQG&ODVV,,IXUFDWLRQLQYROYHPHQWDWWRRWK（lingual）XQGHUZHQWWKHSHULRGRQWDOUHJHQHUDWLYHVXUJHU\E\5HJURWK®
ZLWKRXWDQ\ERQH¿OOLQJPDWHULDOVClinical Procedures and Outcomes: The supportive periodontal therapy（SPT）and radiographic observation have been
SURYLGHGIRUWKLVSDWLHQWIROORZLQJWKHVXUJHU\)LIWHHQPRQWKVDIWHUWKHRSHUDWLRQWKHQHZERQHIRUPDWLRQZDVREVHUYHG
GRPLQDQWO\)URPWKHWKPRQWKWKURXJKWKHWKPRQWKKRZHYHUWKHSDWLHQWZDVXQDEOHWRIROORZWKURXJKWKH637GXHWRWKHRYHUZKHOPLQJORDGRIVRFLDOGXWLHV$VWKLVFDXVHGDUDSLGGHWHULRUDWLRQRISHULRGRQWDOWLVVXHWKHLPPHGLDWHDWWHQWLRQ
ZDV JLYHQ E\ WKH GHQWDO K\JLHQLVW WR UHHQJDJH WKH SDWLHQW ZLWK WKH 6377KLV DFWLRQ KDV UHVXOWHG LQ WKH GUDPDWLFDO
improvement in the patientʼVSHULRGRQWDOFRQGLWLRQ
Conclusion: This case suggests that the long‑WHUP637LVYHU\LPSRUWDQWDWWKHSHULRGRQWDOUHJHQHUDWLYHVXUJLFDOVLWH,WLV
QHFHVVDU\IRUGHQWDOSURIHVVLRQDOVWRSD\DFORVHDWWHQWLRQWRGL൶FXOWVRFLDOVLWXDWLRQVSDWLHQWVPD\IDFHLQRUGHUWRPDLQWDLQWKHSRVLWLYHUHVXOWVRIVXUJHU\

DH‑02

1RQ‑VXUJLFDOSHULRGRQWDOWUHDWPHQWRIDSDWLHQWZLWKJHQHUDOL]HGVHYHUHFKURQLFSHULRGRQWLWLVSatoka Shimizu＊1, Momo Hasegawa15\RNR.DJHQR1<RVKLKLUR,ZDQR1,2
1,ZDQR'HQWDO&OLQLF2'HSDUWPHQWRI3HULRGRQWRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWRU\

Background: :H UHSRUWHG D FDVH RI JHQHUDOL]HG VHYHUH FKURQLF SHULRGRQWLWLV WKDW ZDV DPHOLRUDWHG E\ QRQ‑surgical
SHULRGRQWDOWUHDWPHQWSHUIRUPHGE\DGHQWDOK\JLHQLVWCase: A 61‑year‑old male patient first visited our hospital on December 25, 1994 with a chief complaint of gingival
HQODUJHPHQW*LQJLYDOUHGQHVVDQGHQODUJHPHQWZHUHREVHUYHGLQWKHHQWLUHMDZ3&5ZDV％ , bleeding on probing（BOP）ZDV
％DQG33'DWPPZDV％
7KHSDWLHQWZDVGLDJQRVHGZLWKJHQHUDOL]HGVHYHUHFKURQLFSHULRGRQWLWLVTreatment and Results: Scaling and root planning（653）were performed by coordinating with the dentist regarding the
WLPH RI UHPRYDO RI WKH GHIHFWLYH SURVWKHVLV HWF &OHDQLQJ WRROV ZHUH FKDQJHG DFFRUGLQJ WR WKH FKDQJHV LQ WKH RUDOHQYLURQPHQWGXULQJWUHDWPHQWVXFKDVDSSOLFDWLRQRIDSHULRGRQWDOWUHDWPHQWGHYLFHJLQJLYDOUHFHVVLRQDIWHU653DQG
DSSOLFDWLRQRIDQRUWKRGRQWLFGHYLFH7KH33'RIXSWRPPLPSURYHGWRPP0RUHRYHUPDLQWDLQLQJWKHSDWLHQW V
motivation helped to reduce all the periodontal pockets to＜PPZLWKRXWSHULRGRQWDOVXUJHU\,WKDVEHHQDOPRVW\HDUVVLQFHWKHWUDQVLWLRQWRVXSSRUWLYHSHULRGRQWDOWKHUDS\DQGWKH33'LVPPIRUDOOWHHWKZLWKWKHXVHRI¿YHFOHDQLQJWRROVDQGWKHSHULRGRQWDOFRQGLWLRQKDVUHPDLQHGVWDEOHZLWK3&5RI％DQG%23RI％
Conclusion: ,WFDQEHVXUPLVHGWKDWE\DVVHVVLQJWKHSDWLHQW VSHUVRQDOLW\DQGGHJUHHRILQWHUHVWLQWKHRUDOK\JLHQHVHYHUH
chronic periodontitis can be ameliorated with non‑VXUJLFDOWUHDWPHQWE\HQVXULQJVX൶FLHQWFRPPXQLFDWLRQDQGEXLOGLQJDUHODWLRQVKLSRIWUXVWJSP/JACP Poster Session AAP2020

38

DH‑03

5HVSRQVHWRLQÀDPPDWLRQGXHWRFKDQJHVLQWKHJHQHUDOFRQGLWLRQ
Momo Hasegawa＊1, Satoka Shimizu15\RNR.DJHQR1<RVKLKLUR,ZDQR1,2
1,ZDQR'HQWDO&OLQLF2'HSDUWPHQWRI3HULRGRQWRORJ\1LKRQ8QLYHUVLW\6FKRRORI'HQWLVWRU\

Background: :HUHSRUWHGDFDVHRIUHFXUUHQFHRISHULRGRQWLWLVDVVRFLDWHGZLWKDFKDQJHLQWKHJHQHUDOFRQGLWLRQRIWKH
SDWLHQWCase: A 42‑year‑ROGIHPDOHSDWLHQW¿UVWYLVLWHGRXUKRVSLWDOLQ-XQHZLWKDFKLHIFRPSODLQWRIJLQJLYDOHQODUJHPHQW
EHWZHHQWHHWKDQG5DGLRJUDSKLFLPDJLQJUHYHDOHGORVVRIDOYHRODUERQHEHWZHHQDQG$JUDGH,IXUFDWLRQOHVLRQZDVREVHUYHGDWWKHGLVWDOSRUWLRQRI
Treatment and Results: Basic periodontal treatment was performed for the gingival enlargement between 16 and 17, and all
33'VEHFDPHPPDQGWKH%23DQG3&5VWDELOL]HG（％DQG％ , respectively）；therefore, the transition to SPTZDVPDGHLQ0D\:LWKRQVHWRIKHUSDQJLQDLQ$XJXVWWKHSDWLHQWVKRZHGGHFUHDVHGPDLQWHQDQFHRIRUDO
hygiene, and the gingival enlargement between 16 and 17 recurred with a 6‑PP33'&KDQJHVLQDSSRLQWPHQWVE\WKH
SDWLHQWGXHWRSRRUSK\VLFDOFRQGLWLRQZHUHQRWHGDQGVKHYLVLWHGWKHLQWHUQDOPHGLFLQHGHSDUWPHQWDIWHUH[SHULHQFLQJIDWLJXHIRUVHYHUDOPRQWKV7KHUHVXOWVLQGLFDWHGDGHFUHDVHLQWKHYDOXHVRIWKHWK\URLGJODQGSDUDPHWHUVDQGGHFUHDVHLQ
LPPXQLW\VWUHQJWK&RQVLGHULQJWKHVWDPLQDDQGRUDOFRQGLWLRQRIWKHSDWLHQWWKH637LQWHUYDOZDVVHWWRPRQWKDQG
WKRURXJKRUDOFOHDQLQJJXLGDQFHZDVJLYHQDQGV\PSWRPVDPHOLRUDWHG
Conclusion: %HFDXVHRIWKHUHFXUUHQFHRILQÀDPPDWLRQGXHWRFKDQJHVLQWKHJHQHUDOFRQGLWLRQLQWKHSUHVHQWFDVHDUHYLHZ
of oral cleaning tools was performed and the appropriate SPT interval was continued, thereby leading to stabilization of
V\PSWRPV

DH‑04$FDVHRI‑\HDU‑VXFFHVVIXOSHULRGRQWDOWUHDWPHQWLQFKURQLFSHULRGRQWLWLV
Masami Sato＊.D]X\R,NHGD0DVDKLNR,NHGD,NHGD'HQWDO&OLQLF
Background: 2UDOKHDOWKLVDNH\,1',&$725RIRYHUDOOKHDOWKZHOO‑being and quality of life（:+2）
Case: ,QD¿IW\‑VL[\HDU‑ROGIHPDOHZLWKFKLHIFRPSODLQWRISDLQLQOHIWPRODUVYLVLWHGRXUFOLQLF7KHQDUURZ
gingival width and 4‑PPRIRYHUDOOSHULRGRQWDOSRFNHWVZHUHUHFRJQL]HG;‑UD\H[DPLQDWLRQVKRZHGWKH
KRUL]RQWDOERQHDEVRUSWLRQClinical Procedures and Outcomes: The patient received a diagnosis of chronic periodontitis with crowded dentalarch and started the treatments with basic periodontal treatments, orthodontic treatments, and prosthodontics
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PRWLYHV(VWLPDWLRQRISODTXHFRQWURO3HULRGRQWDOWLVVXHH[DPLQDWLRQ,QWHUYLHZLQJDERXWWKHGLHW
(VWLPDWLRQRIWKHRFFOXVLRQ5HPRYDORIFDOFXOXVDQG2UGLQDU\GHQWDOWUHDWPHQWVLIQHFHVVDU\$WSUHVHQW
the patient turned 91‑\HDUROGDQGGRHVQRWKDYHSUREOHPVWROLYHDORQH6KHKDVEHHQVKRZLQJWKHDFWLYH
DWWLWXGHVWRZDUGWRWKHJRRGKHDOWKDQGNHHSVFRPLQJIRUUHFDOOV2YHUDOOSURELQJSRFNHWGHSWKZDVNHSWDURXQG
2 ‑ 3mm and the plaque control had been well performed（3ODTXH6FRUH ％）Conclusion: Despite getting chronic periodontitis 35‑years ago, this case went well probably because the patient
KDVEHHQKLJKO\PRWLYDWHGWREHKHDOWK,WLVVXJJHVWLYHWKDWGHQWDOK\JLHQLVWKDVLPSRUWDQWUROHVLQ637HVSHFLDOO\GXULQJVWUHQJWKHQPRWLYHVWRZDUGWRRYHUDOOKHDOWKJSP/JACP Poster Session AAP2020

39

The 106th Annual Meeting of the American Academy of Periodontology in collaboration
with the Japanese Society of Periodontology,
and the Japanese Academy of Clinical Periodontology.
Abstracts of JSP/JACP Poster Session
Copyright ©2020 The Japanese Society of Periodontology, Japanese Academy of Clinical Periodontology.
All rights reserved.
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