List of Dissertation Abstract （Environment and Natural Sciences Life Sciences Course）
NameSupervisor

Title

Abstract

In this study, we found several compounds that show selective inhibitory activity depending on the type ofSaki

KazuyukiITOHIRATSUKA

Construction of a novel reporter

luciferase protein. Using this compound, we constructed a novel dual-luciferase reporter assay system. In thisgene assay system using a selectivesystem, the separation of the two kinds of luciferase activities can be carried out regardless of the emission

inhibitor of luciferase activity

wavelength. This novel assay system enables us to conduct a multi-luciferase reporter assay applicalbe tohigh-performance system with wide variety of bioluminescence reporter genes.

I evaluated for diversity and optimum conditions of amino acid separation,

Kei

Yukio

Ogura

YOKOYAMA

Applicability of low-capacity
cation-exchange chromatography
to LC-MS

Low-capacity cation-exchange column co-developed, and found the conditions on which 26 species could beseparated and proteinogenic amino acids could be completely separated.

Also, I have found the separation

conditions of the protein constituent amino acids using formic acid system, which is volatile, instead of
traditional non-volatile phosphoric acid to combine the low exchange capacity cation exchange
chromatography and mass spectrometry.
Yeast is a model organism of eukaryote, by encapsulating in microcapsules, to set the cell

KouheiTakashiOBASE

AMEMIYA

The creation of toxicity evaluation

density in the capsule, was successfully control the synchronization of yeast glycolysis

concept using the glycolytic

oscillating reaction. Yeast degree of synchronization which is densely fixing is high, the degree

synchronization phenomenon ofof synchronization which is fixed to a low density with respect to a low yeast, influence on themicro-encapsulated yeast cell

vibration response of the chitosan is a toxic substance is lower about 40% - 50% it was found.

population

Thus, when cells are present at high density, it was able to offer the possibility to have aresistance to toxicity.

NaoKiyoshi

KAJIMOTO

HONDA

Skeleton Transformation

Treatment of Diels-Alder adducts with oxidants resulted in the formation of amine oxides to give

via Diels-Alder Cycloaddition ofMeisenheimer rearrangement products, followed by skeleton transformation reaction to afford various

Isoindoles and Maleimide

nitrones and hydroxylamines as bioactive compounds.

Multifunctional p-expanded macrocyclic oligothiophene 6-mer is a molecule having interesting structure,
unique optical behavior, and polymorphism. Therefore I have synthesized p-expanded macrocyclic oligo-3,4Synthesis, Structure anddiphenylthiophene 6-mer 6T4A-Ph using modified McMurry coupling reaction of dialdehyde precursor. The

WakanaHiroyukiMorphological alterations of π-molecular structure of 6T4A-Ph was examined by the X-ray crystal structural analysis. The optical properties

KARIYAOTANIExpanded Cyclic Oligo-3,4-of 6T4A-Ph were characterized by the absorption and fluorescence spectra in solution and in the solid state.

diphenylthiophene 6-mer

Furthermore, I discussed about the properties of the 34p-dication 6T4A-Ph2+ which was prepared bychemical oxidation of 6T4A-Ph with Fe(ClO4)3 ･ 6H2O. Morphological changes of 6T4A-Ph by thedifference of recrystallization solvent were observed in the absorption and fluorescence spectra.

The cause of climate is not only anthropogenic factor, such as an increase in carbon dioxide. The
influences of various anthropogenic and natural forces should be taken into account. I examined the influence
ShohKiminori

KAWANOITOH

Statistical analysis of the climateof the solar wind on the climate using the correlation maps between the solar wind activity vs. temperatureeffects of magnetic and radiationdata.

variations of solar activity

As a result I found that the magnitude of the influence of the solar wind on the climate compare wellwith that of the teleconnection patterns such as the AO and PDO. And the solar wind appears to excite theteleconnection patterns to affect the surface temparature.

A novel poly-functional low-capacity cation-exchange packing material was developed for the fast aminoChiharu

Yukio

KIKITSUYOKOYAMA

Preparation of high-selectivity low-acid separation.The amino acid selectivity arised from the versatile interaction to sulfo- and calboxy-

capacity cation exchange column

functional groups on the PS-DVB resin, introduced by Friedel-Crafts reaction, which was successfullyfor Amino acidcontrolled by the concentrations of acylating reagent and Lewis acid. Seventeen underivatized proteinogenic
amino acids were separated in 18 min with a binary gradient elution system using a conventional HPLC.

The troponoid molecules such as tropolone and related 2-substituted tropones are the typical examples ofSynthesis, Properties, andRyuta

Hiroyuki

Photovoltaic Characteristics of theKIRAOtani

D-π-A Type Troponoid Dyes
Containing Thiophene Ring

non-benzenoid compounds which possess pronounced aromatic character. Therefore I have designed andsynthesized eight kinds of novel D-π-A type troponoid dye containing thiophene ring as spacer. I haveinvestigated the molecular structures, the absorption behavior, and the redox properties of these dyes. Inaddition, these dyes have been investigated the photovoltaic characteristics for dye-sensitized solar cells.
Furthermore, the dye-sensitized solar cell device sensitized with the dyes has been fabricated and measured.
All of D-π-A type troponoid dyes containing thiophene ring as spacer showed photovoltaic characteristics.

Crystal structures and solid-state
Shinyaoptical properties of 2,5-diamino-

MATSUMOT

3,6-dicyaopyrazine dyes with smallO

alkyl substituents on the amino

TakumaSAKATA

groupsSolid-state optical properties of 2,5-diamino-3,6-dicyaopyrazine dyes with small alkyl substituents on theamino groups were interpreted by quantum chemical calculations on the basis of the X-ray structure data.
These five derivatives had almost similar electronic structures in a molecular state. In the solid states, on theother hand, significant difference was found in their optical properties. According to the results of semiempirical molecular orbital calculations using the X-ray structure data, the conformations of the amino groups
in the molecule may play a critical role in the solid-state optical characteristics of these derivatives.

In this study, I investigated the use of the high-throughput system of the Agro-infiltration method using aTakuyaKazuyuki

SAKATAHIRATSUKA

Study on development of a highthroughput system for Agroinfiltration method

multi-well plate and examined various conditions to optimize the transgene expression. Unlike theconventional method that requires needleless syringes and fully developed mature leaves for the transient
assay, this method allows infiltration of multiple Agrobacterium samples into small tobacco seedlingssimultaneously. Also, in this system, the use of a multi-well plate and bioluminescence reporters enable us toconduct rapid and accurate evaluation of gene expression in planta with only a limited space.

2H-Chromenes are present in a vast number of natural products and important heterocyclic compounds

YosukeKiyoshi

SHIGEMATSU

HONDA

Novel synthesis of 2H-chromenesby cycloaddition reaction withalkynes and ortho-quinonemethideappeared in anticancer and antioxidant drugs. Especially,

2,3-diaryl-2H-1-benzopyran derivatives arereceived significant attention as selective estrogen receptor adjustment medicines in late years. Most efficient
preparation of 2H-chromenes is an important issue in organic synthesis. In this article,

a novel synthesis ofvarious substituted chromenes from easily available various 5-substituted salicylaldehydes anddiphenylacetylenes is described.

Sulfophenyl carboxylates (SPCs) are known as the biodegradation of Linear alkylbenzene sulfonate (LAS)
widely used as anionic surfactants in a variety of industrial and commercial products. It is reported that theYosuke

Hiroyuki

SHIMODAIRAOTANISynthesis and properties of 5-(4sulfophenyl)heptalate and its
analoguetoxicity for the submarine life of LAS is related to the surface tension. Therefore, I have designed andsynthesized 5-(4-sulphophenyl)heptalate (5C7SPC) and 11-(4-sulphophenyl)tridecalate (11C13SPC) as asingle SPC. 3-Phenylpentanol, as those common precursor, has been synthesized of propiophenone withphosphate ylide. Single 5C7SPC has been synthesized by using the Wittig-Horner reaction, and single
11C13SPC has been synthesized by using the Wittig reaction as the key step. The structure of 5C7SPC or
11C13SPC has been examined by FT-IR and 1H-NMR spectrum.

1,8-Diphenyl-10-mesitylanthracene cyclic dimer with a rigid and symmetrical π-conjugated system is
expected that two biphenylene chains located face-to-face indicate a weak π-π interaction. Herein, theSynthesis, and Properties of 1,8-synthesis, structure, optical properties, and complexation behavior of this dimer were investigated. The title

AtsumiHiroyuki

Diphenyl-10-mesitylanthracene

cyclic dimer was synthesized by using the electron-transfer oxidation of Lipshutz cuprate derived from 10-SHIRAIOTANICyclic Dimer and its Complexation

mesityl-1,8-bis(4-bromophenyl)anthracene. This dimer is a considerably fluorescent, stable molecule. ThisBehavior

dimer like [5,5]paracyclophane forms CT-complex with strong electronic accepter molecule such as DDQ.
The X-ray analysis revealed a unique structure with a small inner cavity which can incorporate a small atomsuch as Cu+. So, I discussed them complexation behavior.

A plant defense gene botrytis-induced kinase1 (BIK1) gene is transcriptionally regulated in response toIdentification and CharacterizationHiroaki

Kazuyukiof a Small Molecule that Induce

SUZUKIHIRATSUKA

Pathogenesis-responsive Gene
Arabidopsis BIK1

disease stresses. However, very little is currently known about the expression mechanisms. To find chemicals
which induce BIK1 gene promoter expression, we developed a high throughput screening (HTS) system
based on bioluminescence reporter gene technology. In this study, I characterized the compound X that acts
BIK1 gene promoter. Using our HTS system, I investigated expression patterns of BIK1 gene promoter inresponse to treatments of compound X. Also, I examined possible chemical priming effect of the compound
X on plant defense gene expressions.

Evaluatione of the physicalRyuta

Kiminori

SEIKE

ITOH

properties Ligand change method
and Green chemical method of
metal organic decomposition
methodThe silver nanoparticles currently active in various fields have been expected, the ligand-change method
is a new synthetic methods, and synthesis was performed using a green chemical method. In addition,
we thermal properties evaluation using a TG / DTA, structural and physical properties of a transmission
electron microscope, the optical property evaluation of using a spectrophotometer. Due to the differenceof the various physical properties in the difference of the protective group in which the mention of thenew findings, such as changes in the changes and the crystal structure of the grain size occurs went.
Spirans are rigid framework, which minimizes the number of possible conformations and consequently

Shunsuke

KiyoshiStereoselective synthesis of 1,1'-TAKUMA

HONDA

disubstituted-2,2'-spirobiindane

benefits in selectivity. Moreover, it is possible to have C2 symmetry in spiro ring system, so it is expected
to give high enantioselectivity. In this work, I had focused on 2,2’-spirobiindane core, and synthesized
1,1’-disubstituted compounds through stereoselective fanctionalization of carbonyls in 2,2’spirobiindane-1,1’-dione.
For high-efficiency production of recombinant proteins in plants, we have developed multigene

ChihiroTSURU

KazuyukiConstruction of multi-gene

HIRATSUK

expression system using several

A

5' untranslated regions of viruses

expression systems using IRES (Internal Ribosome Entry Site). Using transient expression assay, weconfirmed several 5' UTRs (untranslated regions) of viruses are able to increase the IRES-dependenttranslation activity. I investigated whether they show similar activity in transgenic BY-2 cells in thisstudy. Results of bioluminescence moniotoring of luciferase reporter genes indicate that some 5'UTRs
were able to improve bicistronic translation activity in the stable transformants.
Using commercial amino acids analyzer is the most popular method of amino acids analysis, andDaisuke
NOMURA

Yukio

Effects of physics of low-capacity proteinogenic amino acids are separated for an hour. In this study, I have made a study of effects ofYOKOYAM

cation-exchange materials on

physics of low-capacity cation-exchange materials on amino acid selectivity. As a result, 7 nm pore-size

Aamino acid selectivity

base-gel has highly selectivity for amino acids and it was found that it is suitable separating material foramino acids.

Asymmetric epoxidation ofA lot of optically active chromans and coumarans derived fromsubstituted o-allylphenols

plants and microbes are known to have intriguing bioactivities. InShunKiyoshi

catalyzed by Ti-CHA and its

this work, we investigated asymmetric epoxidation of substituted

HIRAOKA

HONDA

application to a divergent

o-allylphenols using Ti catalyst with cyclic hydroxamic acidsynthetic study of chiralligands and its application to a divergent synthetic study of chiralchromanes and coumarans

chromanes and coumarans.

Yeast is a eukaryotic cell. Then, show a common life phenomena with human. Therefore, it can be used as aNaoki

Takashi

HIRAMATSU

AMEMIYA

Quantitative analysis of glycolytic

model cell. Study of glycolysis which is one of the important biological phenomena for animate beings issynchronization phenomena inperformed using the model cell. Glycolytic oscillations in yeast cells show a sharp reaction to an external

yeast cell population

stimulus. Glycolytic oscillation observed in each cell is synchronized with the reagent. It succeeded insynchronizing with newly ethanol and ATP other than acetaldehyde known to be the synchronization action.

We aimed at the proposal of the new concept of " synthesis of gold nanoparticles with recycling thereducing agents " using both formation of gold nanoparticles using hydroquinone (HQ) and an optical
TetsuyaTakashi

HIROTAAMEMIYA

hotochemical synthesis of gold

reduction reaction of benzoquinone (BQ). It is expected to reduce consumption of the reducing agent

nanoparticles with recycling theand Emissions of by-product in the liquid phase reduction method for synthesizing gold nanoparticles.

reducing agents

As a result, our study provides a simple method to synthesize size-controlled gold nanoparticles using
HQ as a reducing agent. Also, we indicated the gold nanoparticle formation using a photochemical
reaction of BQ for the first time.
Lichen is known to accumulate radioactive material in the body. In addition, a lot of examples thatradioactive material is detected under the influence of Fukushima nuclear plant accident excessively inRyoKiminori

Adsorption properties evaluation

Japan are reported. Therefore I thought lichen to have possibilities to be available as adsorption materials

MIYAKEITOH

of the lichen to Sr and Cs

for the radioactive material, and I started a study. At first elucidating adsorption properties andmechanism of the still indistinct lichen, and examining the application to adsorption materials of thestudy was intended.