学術講演会のお知らせ
日時: 2024 年 1 ⽉ 24 ⽇(⽔)15:10 - 17:00
場所: 農学研究科 B101
15:10-15:50
講演タイトルResearch on the Germplasm Creation and Utilization of Chinese cabbage
講師: Dr. WEI Xiaochun (Henan Academy of Agricultural Sciences, China)
講演内容:
Cruciferous vegetable crops covered more than 0.48 M and 0.21 M ha in 2020, all over theworld and China separately (FAOSTA, 2020), and this is expanding rapidly year on year.
Chinese cabbage (Brassica rapa var. pekinensis, 2n = 20) is annually grown as a leafy crop. It isindigenous to China and eastern Asia, where it has been in cultivation since the fifth century. We
engaged in microspore culture and embryo rescue technology in Chinese cabbage since 1989.
Thousands of DH lines were created, and many germplasm resources were innovated based on
those techniques. Fine mapping and clarifying genetic mechanism and gene regulatory networkof important agronomic traits, especially clubroot resistance, male sterility, and tip burn wereperformed. Clubroot disease is a soil-borne disease caused by Plasmodiophora brassicae, andaffects Brassica vegetable production worldwide. We engaged in the fine mapping and themechanism research of CR gene, the miRNAs regulatory network, and the single CR gene line
creation, which will provide new insights into biotic research.

15:50 - 16:20
講演タイトル
Study on Isolated Microspore Culture and Genes Related to Embryogenesis inCabbage
講師: Dr. SU Henan (Henan Academy of Agricultural Sciences, China)
講演内容:
Cabbage (Brassica oleracea L. var. capitata) is an important vegetable crop that is widely
cultivated around the world. Most commercial cabbage cultivars are F1 hybrids to ensure highuniformity and yield. It approximately requires 6 years to produce stable inbred lines in theconventional breeding of parental lines. At the same time, microspore culture is an effective
technique to produce homozygous doubled haploid (DH) lines in only two years, whichsignificantly accelerates the breeding process. However, the embryogenic rate of microsporeculture in cabbage is generally low, and the culture system still needs to be optimized. And themolecular mechanism of microspore embryonic formation is not clear, which limits its further
application in cabbage breeding. In this research, cabbages with different genotypes were usedas the test materials to study the factors affecting microspore embryogenesis rate, QTL locationof embryogenesis, related genes during heat shock-induced microspore embryogenesis. It will
provide a basis for accelerating the large-scale application of microspore culture technology incabbage breeding.

16:20 - 16:50
講演タイトルThe plastid-localized BASS5 plays a critical role in xylem sodium loading andstomatal control regulating plant salt and drought stress
講師: Dr. CHEN Weiwei (Henan Academy of Agricultural Sciences, China)
講演内容: In land plants, root-to-shoot signaling is essential to resist stress by turning extracellular
stimuli into endogenous signals. Sodium ion (Na+) is a critical osmotic substance in plants.
However, the long-distance mechanism of plant roots recognizing the environmental Na+ signals
to rapidly activate the defense system remains elusive. Here, we identified and characterized a bile
acid sodium symporter, GhBASS5, by analyzing the transcriptome data in a salt-tolerant andsalt-sensitive variety and its regulation during the salt and drought response. GhBASS5 is localizedin the plastid membrane and preferentially expressed in the cotton root system. It mediated Na+
loading from roots to shoots in response to salt and drought stress. Overexpression of GhBASS5
accelerated Na+ accumulation in the plant aboveground and showed improved drought tolerance.
Under drought stress, GhBASS5 involved the aliphatic glucosinolates synthesis to promotestomatal closure depending on Na+ in Arabidopsis, and Na+-dependent regulation of stomatalclosure function is conserved in cotton. This study reveals a long-distance signaling pathway by
which plants recognize drought and regulate stomatal closure based on soil moisture Na+concentration, establishing a signaling link between plant stomata from the root system to theleaves.

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