ගྜᡂ䝟䝷䝯䞊䝍䞊䜢฼⏝䛧䛯᳜≀䛾䝇䝖䝺䝇デ᩿ἲ䛾☜❧◊✲ᮇ㛫䠖2015ᖺ䡚‫ڂݜ‬ഐ‫ܢ‬ʀ໪దޭ߻੔P700ϏϧϟʖνʖΝԢ༽ͪ͢৪෼͹঳য়઴ਏஇ৪෼ͺɼҲౕஏ໚ͶࠞΝԾΘͤͳࣙΔҢಊͤΖ͞ͳ͗Ͳ͘͵͏ͪΌɼ৙ͶรԿͤΖਫ਼ү‫ڧ؂‬ͶଲԢ͢ͱਫ਼ଚ͢͵͜Ηͻ͵Δ͵͏ɽࡠ෼͹ਫ਼ࢊͶ͕͏ͱɼ‫ڧ؂‬รಊ͖Δण͜Ζ৪෼͹࢐ԿηφϪηΝ‫ݡ‬գͤ͟ͳɼफྖΝ୉͚͘‫ݰ‬ঙͦ͠ͱ͢Ή͑‫ڬ‬Η͍͗
Ζɽ͖͢͢͵͗ΔɼͨΗΝյඈͤΖన઀͵ࡁക؇ཀྵΝͤΖͪΌͶͺɼଡ͚͹‫͗ݩܨ‬චགྷͲ͍Εɼۛ೧͹ҡ৙‫ـ‬ে͵ʹ΍͠ΔͶͨΗΝࠖೋ͵΍͹ͳ͢ͱ͏Ζɽͨ͹ͪΌɼ৪෼͗ࠕʹ͹ఖౕηφϪηয়‫ڱ‬Ͷ͍Ζ͹͖ɼ্֒͗ਫ਼ͣɼफྖΝఁԾͦ͠Ζ઴ͶາષͶ‫ݗ‬எͤΖͪΌ͹ηφϪηਏஇ๑͗‫ٽ‬ΌΔΗΖɽըʓͺɼ͞ΗΉͲ͹ޭ߻੔‫ڂݜેخ‬ΓΕɼ৪෼ͺޭ߻੔Ͷ෈న͵ηφϪη‫ڧ؂‬Ͷര͠ΗΖͳɼʰP700࢐Կʱͳ‫ͻݼ‬ΗΖɼ
࢐Կ্֒͹‫ݬ‬Ҿͳ͵Ζ‫׈‬੓࢐ો͹൅ਫ਼‫΃ݱ‬͹ుࢢட੷Νඈ͜ΖԢ౶ΝͤΖ͞ͳΝ‫ݡ‬ड़͢ͱͪ͘ɽͨ͹எ‫ݡ‬ΝԢ༽͢ɼʰP700
࢐Կʱ͹଎ఈٗढ़Νɼ৪෼͹ηφϪηਏஇ๑ͳ͢ͱ‫ͤ༽׈‬Ζ͞ͳΝ໪దͶ‫ڂݜ‬Νߨͮͪɽࣰ‫֕ݩ‬གྷ‫ڧ؂‬ηφϪηԻౕɼ‫ף‬૫ɼetc.

࢐Կ্֒फྖఁԾ্֒͗ਫ਼ͣΖ઴ͶɼາષͶηφϪη‫ݗ‬எ҈ఈͪ͢ࡠ෼ਫ਼ࢊਈ‫ރ‬୉಼ָ͹ҡ͵Ζӭ཈ড়݇͹า৖Ͷ͕͏ͱɼαϞάΝࡁക͢ӭ཈ܿ๣‫ڧ؂‬Ͷ͕͜ΖP700࢐ԿԢ౶Ͷͯ͏ͱմੵͪ͢ɽ݃Վͳߡࡱ
ਦ1; ਈ‫ރ‬୉ָߑ಼͹ҡ͵ΖαϞάา
৖ɽB,CஏۢͺྣંͤΖۢժͲ͍ΕɼCͶ͹Ίࢬ඄ͪ͢ɽ

ਦ3; ೽झ‫ޛ‬11ुؔ͹֦า৖͹αϞά͹ਫ਼үͳ‫ף‬෼॑ྖɽਈ‫ރ‬୉಼ָͶ3ϴॶ͹ҡ͵Ζࣰ‫ݩ‬า৖(A-C)Ν
અ͜ɼαϞά(೸ྜྷ61ߺ)Νࡁകͪ͢ɽCஏۢͶ͹Ίඬ६ద͵ࢬ඄Νߨ͏ɼαϱφϫʖϩۢͳͪ͢ɽ೽झ‫ޛ‬3ुؔ͹য়ସͲͺɼໃࢬ඄͹AɼB
͹ஏۢͲͺɼ‫ݺ‬ର‫ף‬෼॑Ͷ༙қ͵ࠫͺ͵͖ͮͪ(ਦ1)ɽ͖͢͢͵͗Δɼͨ͹ࡏͶ଎ఈͪ͢
P700࢐Կ͹ౕ߻͏ͺɼAஏۢͲB,Cͳͺ໎Δ͖ਦ2; ೽झ‫ޛ‬3ुͶҡ͵Ε߶͏஍Νࣖͪ͢(ਦ2)ɽͨ͹‫ޛ‬ɼαϞؔ͹֦า৖͹αά͹ࡁകΝ11ु໪ΉͲକͪ݃͜ՎɼAஏۢ͹αϞά͹ਫ਼үͳ‫ף‬ϞάͺBஏۢ͹αϞάΓΕ΍໎Δ͖Ͷਫ਼ү͗ཊ෼॑ྖ(৏)ͳɼ
੏ ͠ Η ͪ ( ਦ 3) ɽ ೽ झ ‫ ޛ‬3 ु ؔ ໪ ͹ ஊ ֌ Γ ΕP700 ࢐ Կ ଎ ఈP700 ࢐ԿͶͺ໎֮͵ࠫ͗ਫ਼ͣͱ͕Εɼӭ཈ܿ͹݃Վ(ࠪ)ɽ
๣ηφϪηΝɼΉͫ໪ࢻͲͺΚ͖Δ͵͏ॵ‫غ‬ஊ֌Ͳ‫ݗ‬எͲͪ݃͘Վͳ͵ͮͪɽͨ͹‫ޛ‬͹մੵ͖ΔɼAஏۢͺMn(ϜϱΪϱ)ܿ๣ౖৗͲ͍Ζ͞ͳ͗Κ͖ͮͪɽҲ๏ͲɼBͳCͶͺࠫ͗͵͖ͮͪ͞ͳ͖Δɼಝఈ͹‫ݫ‬ો͹ܿ๣Ν‫ݗ‬எͲͪ͘ͳߡ͓ΔΗΖɽࠕ‫ޛ‬ɼʹ͹Γ͑͵‫؂‬‫ڧ‬ηφϪηΉͪͺ৪෼झͶ͕͏ͱɼP700࢐Կ଎ఈ༙͗ް͵ηφϪηਏஇ๑ͳ͵Ζ͹͖ɼ໎Δ͖ͶͤΖ͞ͳ͗՟ୌͲ͍Ζɽ⚄ᡞ኱Ꮫ኱Ꮫ㝔 ㎰Ꮫ◊✲⛉ ᳜≀ᰤ㣴Ꮫ◊✲ᐊ୕Ꮿ ぶᘯ 㐃⤡ඛ䠖cmiyake@hawk.kobe-u.ac.jp

൅න࿨ช

1. Shimakawa G, Shaku K, Nishi A, Hayashi R, Yamamoto H, Sakamoto K, Makino A, Miyake
C. (2015) FLAVODIIRON2 and FLAVODIIRON4 proteins mediate an oxygen-dependent
alternative electron flow in Synechocystis sp. PCC 6803 under CO2-limited conditions.
Plant Physiol. 167: 472-480.
2. Sejima T, Hanawa H, Shimakawa G, Takagi D, Suzuki Y, Fukayama H, Makino A, Miyake C.
(2016) Post-illumination transient O2 -uptake is driven by photorespiration in tobacco
leaves. Physiol. Plant. 156: 227-238.
3. Shaku K, Shimakawa G, Hashiguchi M, Miyake C. (2016) Reduction-Induced Suppressionof Electron Flow (RISE) in the Photosynthetic Electron Transport System of Synechococcus
elongatus PCC 7942. Plant Cell Physiol. 57: 1443-1453.
4. Takagi D, Takumi S, Hashiguchi M, Sejima T, Miyake C. (2016) Superoxide and Singlet
Oxygen Produced within the Thylakoid Membranes Both Cause Photosystem I
Photoinhibition. Plant Physiol. 171: 1626-1634.
5. Shimakawa G, Akimoto S, Ueno Y, Wada A, Shaku K, Takahashi Y, Miyake C. (2016)
Diversity in photosynthetic electron transport under [CO2]-limitation: the cyanobacterium
Synechococcus sp. PCC 7002 and green alga Chlamydomonas reinhardtii drive an O 2dependent alternative electron flow and non-photochemical quenching of chlorophyll
fluorescence during CO2-limited photosynthesis. Photosyntesis Res. 130: 293-305.
6. Shimakawa G, Shaku K, Miyake C. (2016) Oxidation of P700 in Photosystem I Is Essentialfor the Growth of Cyanobacteria. Plant Physiol. 172: 1443-1450.
7. Shimakawa G, Matsuda Y, Nakajima K, Tamoi M, Shigeoka S, Miyake C. (2017) Diverse
strategies of O2 usage for preventing photo-oxidative damage under CO2 limitation
during algal photosynthesis. Sci. Rep. 7: 41022.
8. Shimakawa G, Ishizaki K, Tsukamoto S, Tanaka M, Sejima T, Miyake C. (2017)TheLiverwort, Marchantia, Drives Alternative Electron Flow Using a Flavodiiron Protein toProtect PSI. Plant Physiol. 173: 1636-1647.
9. Takagi D, Ishizaki K, Hanawa H, Mabuchi T, Shimakawa G, Yamamoto H, Miyake C. (2017)
Diversity of strategies for escaping reactive oxygen species production within
photosystem I among land plants: P700 oxidation system is prerequisite for alleviating
photoinhibition in photosystem I. Physiol. Plant. 161: 56-74.
10. Shimakawa G, Miyake C. (2018) Respiratory terminal oxidases alleviate photo-oxidative
damage in photosystem I during repetitive short-pulse illumination in Synechocystis sp.
PCC 6803. Photosynthe. Res. 137: 241-250.
11. Kadota K, Furutani R, Makino A, Suzuki Y, Wada S, Miyake C. (2019) Oxidation of P700
Induces Alternative Electron Flow in Photosystem I in Wheat Leaves. Plants, 8 (6).
ଠɼ20ๅ
ָճ൅න

(2017೧ʛ)ࠅࡏָճ7݇ࠅ಼ָճ

17݇‫ڠ‬ಋ‫ڂݜ‬઎‫౐ښ‬୉ָɼ౨‫ښ‬༂Ռ୉ָɼ‫آ‬घ୉ָɼ౨๼୉ָɼ෾ޭ‫ࣞהحܯ‬ճऀ‫ڂݜ‬ඇCREST [৪෼‫݊ئ‬੓]‫׈‬੓࢐ોਫ਼੔ཊ੏εητϞ͹ඉഃշ඲Ճ‫ܧ‬͹ཱི֮ͳϓΡʖϩχ΃͹Ԣ༽ʛ‫ؽث‬૥‫غ‬ਏஇεητϞ͹ߑ஛ʛ(2015-2020)