March 17, 2023
To whom it may concern
Company Name

Nakamura Choukou Co.

Representative

President ＆Name

Chairman of Board

Makoto Inoue(Code No.: 6166 Tokyo Stock Exchange - Growth Market)

Notice of Progress on Joint Research with Chiba University

As disclosed on September 30, 2022, we have been conducting a joint research with the Laboratoryof Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Chiba University (Prof.
Tetsuhiro Nemoto) using our flow synthesis system on the synthesis method of PIP（※1）, an organic
compound that is said to function effectively in the field of drug discovery research for cancer
drugs with fewer side effects. At this time, we are pleased to announce that we have completed
the synthesis of the main fragment（※2）to obtain the long-chain PIP structure, which was theinitial objective of this joint research, and to inform you of the results of the research andour future plans, as follows;1.

Major Research Results(1) Reproducibility was confirmed in synthesis tests of multiple dimers, trimers, andtetramers.
[An example of a dimer]PP[An example of a trimmer]

PPP

1

[An example of a tetramer]

PPPP(2) The short synthesis times and high yields observed in all cases indicate that PIP
synthesis is feasible in a low-cost, high-volume production system.
2.

Policy for Future Activities
The outcome of this joint research is the acquisition of a synthesis method that canstably provide PIP structures, organic compounds whose functions are expected in the fieldof drug discovery research, at a low cost.
We will continue to conduct joint research with the Laboratory of Medicinal Chemistry,
Graduate School of Pharmaceutical Sciences, Chiba University, using our flow synthesizer,
and also provide technical reports on the synthesis and samples of major fragments tocompanies and research institutions related to PIP in order to raise awareness of theexcellent functions of PIP and stimulate demand.

3.

Future Outlook
The impact of this joint research on our business results for the fiscal year ending
March 31, 2023 is expected to be negligible.
(*1) PIP○ What is PIP (Pyrrole Imidazole Polyamide)?
It is a generic term for compounds in which a pyrrole backbone and an imidazole backbone arelinked by amide bonds in a specific order, and each of these pyrrole and imidazole backbones canhydrogen bond to a specific base structure portion in DNA.
Since PIP interacts with specific base sequences of DNA, it is possible to develop drugs that act
on DNA by binding medicinal ingredients to PIP, and it is expected that drugs with fewer sideeffects, such as cancer drugs, can be developed. However, the existing synthesis of PIP is notefficient in terms of material yield, etc., and the price of PIP is very expensive due to thetime and labor required for synthesis, which has hindered the development of research using PIP.
･Pyrrole･Imidazole

Contains one nitrogen atom

Organic compound with a five-

Organic compounds with five-membered ring structure containing

membered ring structure

two nitrogen atoms(*2) Fragment
A state in which originally continuous information (in this case, an organic compound linked
by amide bonds) is broken up into small discontinuous blocks.

2