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Background ========== Monoclonal antibodies have been used to target cytokines and proinflammatory factors to treat a range of diseases.
The discovery of these proteins and the therapeutic potential of monoclonal antibodies has created a significant role for the development ofrecombinant antibody fragments. We were interested in the development of monoclonal antibodies against proinflammatory cytokines and theirability to reduce levels of these cytokines in vivo. Methods ======= We have investigated the potential of the recombinant monoclonal
antibodies in reducing the inflammation in an immunological mouse model of arthritis. The antibodies were injected into the miceintraperitoneally, and a control group was administered 10 mg of the anti-IL-10 antibody clone H.10, which we have previously reported toreduce immune-mediated diseases. During the course of treatment and within 48 hours after the last injection, we measured the levels of IL-17,
TNF-α and IL-1β cytokines in the serum by ELISA. Results ======= Our results show that the recombinant monoclonal antibodies reduced
the levels of the cytokines IL-17, TNF-α and IL-1β in the mice sera with two different patterns of administration, with the effect of theantibodies being more pronounced when the antibodies were administered as early as possible during the treatment time-period and with asingle administration. Conclusions =========== These data confirm the potential of recombinant monoclonal antibodies as therapeutic
agents in the prevention of autoimmune diseases, based on our results they have the potential of being used as a treatment for arthritis andautoimmune disease. Effect of different surface treatments on the roughness, hardness and fluoride uptake of dental enamel. The objective of
the present in vitro study was to determine the effect of different surface treatments on the roughness, hardness and fluoride uptake of humanenamel. Thirty-five human premolars were sectioned mesiodistally to obtain a flat surface on the enamel. The specimens were randomly
assigned to five groups (n = 7), each group representing different surface treatments. Surface treatments included air abrasion, sandblasting,phosphoric acid etching, 10% polyacrylic acid gel application and ultra sound, followed by silane priming. Each specimen was subjected toroughness, microhardness and fluoride uptake measurements (quantitative micro-photomacrography). Surface roughness increased significantlyin the untreated f678ea9f9e
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