IBioIC Introduction to Bioinformatics
Documentation
ReleaseLeighton Pritchard, Sue Jones, Peter Cock

Mar 12, 2018

This site’s contents:

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What do I need to do before the course?
1.1 Installation for Course Attendees . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.2 Installation for Tutors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.3 Testing the Installed Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.4 Installing Anaconda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.5 Installing ARTEMIS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.6 Installing BLAST . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.7 Installing GIT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.8 Installing JALVIEW . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.9 Installing JAVA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.10 Installing JMOL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.11 Installing MUSCLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.12 Installing VirtualBox . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.13 Indices and tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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IBioIC Introduction to Bioinformatics Documentation, Release

This documentation describes software installation and setup for attendees and tutors at the March 2018 presentationof the IBioIC Introduction to Bioinformatics course, for industrial biotechnology postgraduates.

This site’s contents:

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IBioIC Introduction to Bioinformatics Documentation, Release2

This site’s contents:

CHAPTER

1What do I need to do before the course?

You need to ensure that you have the course materials and required software to hand on your own machine.
Attention: COURSE ATTENDEES:
Please follow the installation instructions at Installation for Course Attendees.

Attention: COURSE TUTORS:
Please follow the installation instructions at Installation for Tutors.
You should have been provided with a static copy of the installation instructions by the course organisers, but online
copies of the course materials and a prebuilt virtual machine are also available:• Course homepage• Course repository• Ubuntu Virtual Machine

1.1 Installation for Course Attendees
This document will describe the process for setting up and testing course materials, in preparation for attendance at
the workshop.
Two different setups are described:
1. Installation of all components on your own local machine
2. Download and use of a Linux virtual machine3

IBioIC Introduction to Bioinformatics Documentation, Release

Either one of these will prepare your machine for the workshop.
We prefer that attendees install materials on their own laptops for the course, as the software and learning materials
will then persist and be usable/re-usable on your own machine after the course itself is complete. The virtual machineis a “Plan B” that should be usable on any machine, and is intended as the fallback in case of installation problems on
your own laptop.
Important: You should install all the software and/or the virtual machine before you attend the course. This will saveyou, and everyone else at the course, time at the beginning of the first day, and give more time to explore advanced
topics in the workshop.
We will offer an online hangout to help with installation problems, just before the course. Your course organiser will
provide information about this.
The process of installing the required software on your machine is as follows:
1. Install Anaconda on your computer (if it is not already present).
2. Install git on your computer (if it is not already present).
3. Obtain the course materials.
4. Install Python requirements.
5. Install third-party software tools.
6. Test the installation.
The process of installing the virtual machine is as follows:
1. Install VirtualBox on your computer.
2. Download the course virtual machine and open it in VirtualBox.

1.1.1 1. Install all components on your local machine
1. Install Anaconda on your computer
For ease of installation and consistency, we install as much as is practical using the Anaconda environment. This iscross-platform software that works on Windows, macOS and Linux, and provides several essential components for thecourse, such as Python and the common Jupyter notebook interface that will be used.
If you do not already have Anaconda installed on your system, please follow the instructions at the page below:• Anaconda installation
2. Install git on your computer
If you do not have a working copy of git installed on your machine, install one now by following instructions at thepage below.
• git installation
Note: If you are using Windows, you will be installing git bash which, as well as git, provides the Bashterminal environment that you will be using during the course. This provides an experience very similar to working at
the terminal in Linux/macOS.

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3. Install the course materials
You will need to clone the course material repository to your own machine.
Using the terminal1 , navigate to a convenient location (e.g. your Desktop). Then clone the course repository withthe command:
git clone https://github.com/widdowquinn/2018-03-06-ibioic.git

Note: This will create a new directory called 2018-03-06-ibioic, containing the course materials.
Change to the course material directory in your terminal with the command:
cd 2018-03-06-ibioic

4. Install Python requirements
To install the Python module requirements for the course, issue the command below in the terminal1 :pip install -r requirements_students.txt

5. Install third-party software
BLAST and MUSCLE do not require JAVA, and can be set up independently:
1. Install BLAST
BLAST is a tool for searching with a protein or nucleotide sequence against a database of other biological sequences.
• BLAST installation instructions
2. Install MUSCLEMUSCLE is a program for multiple sequence alignment.
• MUSCLE installation instructions
ARTEMIS, JALVIEW and JMOL require the JAVA VM, so JAVA must be installed first:
3. Install JAVAJAVA is a programming language that runs on a virtual machine (the JVM). Several bioinformatics tools are written
in JAVA, and require the JVM to be installed in order to run.
• JAVA installation instructions
1 The terminal means git

bash on Windows, and Bash on Linux/macOS.

1.1. Installation for Course Attendees

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4. Install ARTEMISARTEMIS is a genome sequence browser and editor.
• ARTEMIS installation instructions
5. Install JALVIEWJALVIEW is a sequence alignment viewer and editor.
• JALVIEW installation instructions
6. Install JMOLJMOL is a program for visualising biological molecules (e.g. proteins).
• JMOL installation instructions
7. Test the tools/materials
To make sure that the downloaded tools are installed and working on your machine, please follow the instructions on
the testing your installation page.
• Testing your installation

1.1.2 2. Download and use a Linux virtual machine
1. Install VirtualBox on your computer
VirtualBox is a program that allows you to run virtual machines on your own computer. Virtual machines aresoftware implementations of operating systems that run as if they are a separate computer.
We have provided a virtual machine pre-loaded with software and course materials, as a fallback in case of installationdifficulties on your own machine. To install the VirtualBox program, please follow the instructions on the linked
page.
• Install VirtualBox
2. Download and import the course virtual machine
We provide a Linux virtual machine pre-loaded with course materials and the required software, which can be used toparticipate in the workshop.
Attention: The virtual machine file is very large (11GB) and should be downloaded well in advance of theworkshop, if you think you may need to use it!
Click on the badge below to go to the virtual machine download page: Click on the link for 2018-03-06-ibioic.
vdi to download the virtual machine in a suitable location.

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Warning: This may take some time to download!
Click on the link for 2018-03-06-ibioic.vbox to download the VirtualBox file in the same location as the.vdi file.
Add the virtual machine.
• Click on Machine in the VirtualBox menu bar• Click on Add• Navigate to the .vbox file you just downloaded, and select it• Click on OpenYou should see the 2018-03-06-ibioic machine in the list at the left hand side of the application.
Start the virtual machine.
• Select the new IBioIC virtual machine (2018-03-06-ibioic)• Click on the Start button in VirtualBox
The virtual machine will start as a new window, and appear to be booting up. When this process is complete, it will
present you with a login screen. Use the following credentials to log in:• Username: ibioic• Password: ibioic-course
On successful login, you will see a standard Ubuntu desktop, and will be ready to begin the course.

1.2 Installation for Tutors
This document will describe the process for setting up and testing course materials, in preparation for delivery to aclass.
Two setups are described:
1. Installation on the local machine
2. Installation on a Linux virtual machine
We prefer that students install materials on their own laptops for the course, as the software and learning materials willthen persist and be usable/re-usable after the course itself. The virtual machine approach is a “Plan B” backup optionthat should be workable on any machine, and is intended as the fallback in case of severe installation problems.
Important: Each new course presentation should be prepared in its own repository. Following the practice of The
Carpentries we have adopted the convention of naming the course repository by date as YYYY-MM-DD-ibioic.
The process of installation for course preparation for tutors is as follows:
1. Prepare a repository for your presentation (this may already have been done by a colleague)
2. Create a new VirtualBox VM for the course
3. Clone the repository to your machine (your laptop, and the VM)
4. Prepare a virtual machine for the course
5. Install required software for the course
1.2. Installation for Tutors

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6. Test the materials
7. Upload the working VM to ‘Zenodo‘_

1.2.1 1. Prepare a new repository
Note: If one of your colleagues has already created/imported a repository for your presentation, you can skip thispart.
When creating a new repository for a new course presentation, please use the GitHub Importer and provide an existing
repository URL to build from, rather than forking an existing repository.
1. Log in to GitHub
2. In the upper right-hand corner of any page, click + and then Import Repository
3. Provide the URL of a previous IBioIC training course repository
4. Choose an account or organisation to own the repository
5. Choose a name for the repository (YYYY-MM-DD-ibioic works for us)
6. Specify that the repository should be public
7. Click on Begin Import
8. You will receive an email informing you when the repository has been imported
9. Inform the other tutors about the repository and/or invite them as collaborators

1.2.2 2. Create a new VirtualBox VM
If it is not already available, download and install VirtualBox on your machine. This is a free-to-use general-purpose
full virtualizer for x86 hardware, capable of running a virtual machine for use by students on the course.
Tip: The virtual machine for the course may be large, so can be prepared for download by the students well inadvance,and include a working git installation, so the student can pull an up-to-date copy of the course materials
during the course. What is important is that the supporting software are available and can be run on the student’s
machine• VirtualBox installation
Once VirtualBox is installed, create a new Ubuntu VM with the same name as your repository for the course presentation.
• Create VirtualBox VM

1.2.3 3. Clone the repository to your machine
Attention: These instructions should be followed to reproduce the repository and required software on both the
VirtualBox VM prepared above, and on your own machine

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Warning: If you do not have a working copy of git installed on your machine, install one now. This will be
required to maintain and publish your repository materials.
• git installation
You should clone the repository to your own machine, with the command:
git clone <REPOSITORY URL>

where <REPOSITORY URL> is the repository you have just imported.
Finally, change directory to the root of the new repository.

1.2.4 4. Prepare a conda virtual machine for the course
For ease of installation and consistency, we install as much as is practical using the Anaconda environment. This iscross-platform on Windows, macOS and Linux, and provides several essential components for the course, such as
Python and the common Jupyter notebook interface that will be used.
Note: If you do not already have Anaconda installed on your system, please follow the instructions:• Anaconda installation
Create a new Anaconda environment
With Anaconda installed, create a new environment with:conda create --name <ENVIRONMENT_NAME> python=3.6

Accept all the installation options presented.
Tip: We recommend naming your environment after your repository, using something like 2018-03-06-ibioic
as your <ENVIRONMENT_NAME>
Then, activate the environment withsource activate <EVIRONMENT_NAME>

You should see your terminal prompt change to include the environment name. This is a reminder that you are working
within the specific Anaconda environment for the course materials.
Some tools are useful to us as tutors, for preparing and managing the course materials. These are specified in the filerequirements_tutors.txt, and should be installed now with the command:
pip install -r requirements_tutors.txt

1.2.5 5. Install required software for the course
We prefer that students use their own laptops for course delivery, and we aim to match the students’ installation
experience here, as closely as possible.

1.2. Installation for Tutors

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Python dependencies
Although we could install most of the python requirements with Anaconda, some of the packages are not available on
Windows with this approach, so we install using pip instead:pip install -r requirements_students.txt

Third-party software
BLAST and MUSCLE do not require JAVA, and can be set up independently:• Install BLAST• Install MUSCLEARTEMIS, JALVIEW and JMOL require the JAVA VM, so JAVA must be installed first:• Install JAVA
• Install ARTEMIS• Install JALVIEW• Install JMOL

1.2.6 6. Test the materials
1.2.7 7. Upload the VM to ‘Zenodo‘_

1.3 Testing the Installed Tools
You would have tested some of these during the installation, but just in case, we’ll recap. Note that the exact version
numbers need not match perfectly.
1. Open a terminal window (Git Bash on Windows)
2. Confirm Conda is installed by running:$ conda --version
conda 4.4.10

3. Confirm Git is installed by running:$ git --version
git version 2.16.2.windows.1

4. Confirm Python 3 from Anaconda is installed by running:$ python --version
Python 3.6.4 :: Anaconda, Inc.

5. Confirm the Python libraries we will be using are installed by running:$ python -c "import Bio; import bioservices; import seaborn; import reportlab"

6. Confirm Muscle is installed by running:

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IBioIC Introduction to Bioinformatics Documentation, Release$ muscle -version
MUSCLE v3.8.31 by Robert C. Edgar

7. Confirm NCBI BLAST+ is installed by running:$ blastn -v
USAGE
blastn [-h] [-help] ...

8. You should have already tested that the Java applications can start.

1.4 Installing Anaconda
1.4.1 Linux Anaconda installation
1. Open https://www.anaconda.com/download with your web browser.
2. Download the Python 3 64-bit installer for Linux.
3. Open a terminal window.
4. Type bash Anaconda3- and then press tab. The name of the file you just downloaded should appear. If it
does not, navigate to the folder where you downloaded the file, for example with: cd ~/Downloads. Then,try again.
5. Press [Enter]. You will follow the text-only prompts. To move through the text, press the [space] key.
Type yes and press [Enter] to approve the license. Press [Enter] to approve the default location for
the files. Type yes and press [Enter] to prepend Anaconda to your ${PATH} (this makes the Anaconda
distribution the default Python).
6. Close the terminal window.

1.4.2 macOS Anaconda installation• Anaconda Video Tutorial (macOS)
1. Open https://www.anaconda.com/download with your web browser.
2. Download and run the Python 3 installer for OS X.
3. Install Python 3 using all of the defaults for installation.

1.4.3 Windows Anaconda installation• Anaconda Video Tutorial (Windows)
1. Open https://www.anaconda.com/download with your web browser.
2. Download and run the Python 3 installer for Windows.
3. Install using defaults for installation except• make sure to check Add Anaconda to my PATH environment variable (this is required to workwith git bash)• make sure to check Register Anaconda as my default Python 3.6.

1.4. Installing Anaconda

11IBioIC Introduction to Bioinformatics Documentation, Release• you can skip installation of VSCode (though it is a very nice tool)
Warning:dows.

You must select the Register Anaconda as my default Python 3.6 option on Win-1.4.4 Post-installation
We need to add some Anaconda channels, which is done by issuing the following commands in the terminal1 :conda config --add channels defaults
conda config --add channels conda-forge
conda config --add channels bioconda

Note: On Windows, you can install these channels using the Anaconda Navigator, a graphical tool provided
through your Start Menu on that platform.

1.4.5 Starting the Anaconda prompt (Windows-only)
Once Anaconda has been installed, you can start a terminal that “sees” the Anaconda Python installation as follows:
1. Click on the Start/Windows menu
2. Go to Anaconda
3. Scroll down (if necessary) to Anaconda Prompt
4. Click on Anaconda Prompt
This will give you a terminal window where you can run the commands to install Python modules and create conda
environments.
Note: We will not be using the Anaconda Prompt as our terminal in this workshop as it does not, by default,
understand the Bash commands we will be using to navigate the system.

1.5 Installing ARTEMIS
Warning: ARTEMIS requires JAVA (installation instructions)
We use the genome browser and editor ARTEMIS at several points in the course. This can be installed following the
instructions in the ARTEMIS manual, or as described below:
1 The terminal means either your bash terminal (macOS/Linux), or the git

12bash terminal (Windows)

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1.5.1 macOS installation
The latest version of Artemis is available as a .dmg installer:• Artemis installer
To install, download the file, uncompress it on your machine, and follow the instructions.

1.5.2 Linux installation
The Linux version of ARTEMIS is available as a compressed .tar.gz file:• Artemis for LinuxThis can be downloaded and extracted to produce the artemis/ directory structure. This can be moved a suitablelocation (e.g. your home directory) with:
mv ./artemis ~/artemis

and the artemis program added to ${PATH} with:
export PATH=${PATH}:~/artemis/

To make this change persist in your system, you should add this line (export PATH=...)
bash_profile file.

to your ~/.

1.5.3 Windows installation
Download the ARTEMIS .jar file:• Artemis for Windows
and place it somewhere accessible (e.g. your Desktop).
To start ARTEMIS, double-click on the artemis.jar file.

1.6 Installing BLAST
We use the sequence search tool BLAST at several points in the course. This can be installed as follows:

1.6.1 Linux/macOS installation
BLAST is available through Bioconda
conda install blast

1.6.2 Windows installation
1. Download ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/2.7.1/ncbi-blast-2.7.1+-win64.exe with your web
browser. If that FTP links does not work, try HTTP instead http://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/2.7.1/ncbi-blast-2.7.1+-win64.exe

1.6. Installing BLAST

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2. Run this installer using the defaults, it should put BLAST under C:\Program Files\NCBI\blast-2.
7.1+
3. Test one of the BLAST programs can be run by executing the command blastn -h in the Git Bash terminal.

1.7 Installing GIT
1.7.1 Linux git installation
git should be available in the terminal for your distribution. You can test this by issuing git --version:$ git --version
git version 2.15.0

If it is not available already, then you should try installing git from your distribution’s package manager, forexample with sudo apt-get install git or sudo apt install git (Debian/Ubuntu) or sudo dnf
install git (Fedora).

1.7.2 macOS git installation
git should be available in the terminal. You can test this by issuing git --version:$ git --version
git version 2.15.0

If it is not available, then you should install the most recent version of the mavericks installer from the Git Mavericks
list

1.7.3 Windows git installation
If not installed already, we recommend the use of the Git Bash shell throughout the course, for Windows users.
This provides a consistent environment equivalent to the powerful Linux and macOS Bash shells.
• Git Bash video tutorial
1. Download the Git for Windows installer.
2. Run the installer and follow the steps bellow:(a) Click on Next.
(b) Click on Next.
(c) Change the editor from default vim to use nano instead.
(d) Keep ‘‘Use Git from the Windows Command Prompt‘‘ selected and click on ‘‘Next‘‘. If you
forgot to do this programs that you need for the workshop will not work properly. If this happens
rerun the installer and select the appropriate option.
(e) Click on Next.
(f) Click on Next.
(g) Keep ‘‘Checkout Windows-style, commit Unix-style line endings‘‘ selected and click on ‘‘Next‘‘.
(h) Keep ‘‘Use Windows’ default console window‘‘ selected and click on ‘‘Next‘‘.

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IBioIC Introduction to Bioinformatics Documentation, Release(i) Click on Install.
(j) Click on Finish.
3. If your HOME environment variable is not set (or you don’t know what this is):(a) Open command prompt (Open Start Menu then type cmd and press the [Enter] key)(b) Type the following line into the command prompt window exactly as shown: setx HOME"%USERPROFILE%"(c) Press [Enter], you should see SUCCESS: Specified value was saved.
(d) Quit command prompt by typing exit then pressing [Enter]
This will provide you with both git and bash in the Git Bash program, which you can start from the Start Menu
on your Windows machine.
git should be available in the Git Bash terminal. You can test this by issuing git --version:$ git --version
git version 2.16....

1.8 Installing JALVIEW
Warning: JALVIEW requires JAVA (installation instructions)
We use the sequence alignment viewer JALVIEW at several points in the course. This can be installed as follows:

1.8.1 Linux/macOS installation
JALVIEW is available through Bioconda (which was set up above):conda install jalview

Alternatively, on macOS you can also download the installer .dmg file, open it and follow the instructions:• JALVIEW installer for macOS

1.8.2 Windows installation
JALVIEW can be launched directly from the link below (if JAVA is installed):• JALVIEW launch

1.9 Installing JAVA
The tools ARTEMIS, JALVIEW, and JMOL are all Java-based, and require the JVM to run. Java is usually present on
Linux (and Windows?) machines, but is not available on macOS by default

1.8. Installing JALVIEW

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1.9.1 macOS installation
The latest version of Java is available as a .dmg installer:• Java VM installer
To install, download the file, open it and follow the instructions.

1.9.2 Linux installation
Java is likely already installed on your machine. You can test whether it is by issuing the following command at theterminal:
java -version

This should return a short account of the Java version. If it does not, then please follow the instructions at the page
below:• Java Installation for Linux

1.9.3 Windows installation
Java is likely already installed on your machine. If it is not, please follow the instructions at the page below:• Java Installation for Windows

1.10 Installing JMOL
Warning: JMOL requires JAVA (installation instructions)
We use the protein structure viewer JMOL in the course. This can be installed following the instructions on the JMOL
website.

1.10.1 Download JMOLJMOL is provided as a single JAVA application for all operating systems. To download it, click on the link below.
• JMOL latest version
Clicking on the link above should download a .zip or .tar.gz file, which can be extracted, producing a directory
containing the JMOL application.

1.10.2 Running JMOLTo start JMOL, open the parent directory in your file explorer (e.g. Finder on macOS), and double-click on thejmol.jar file.
Note: On macOS you may not be permitted to run this executable, as the program is not signed. If this is the case,open System Preferences -> Security & Privacy and click on the General tab. In the lower section

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IBioIC Introduction to Bioinformatics Documentation, Releaseof the window, you should see an option to trust the file jmol.jar. Accept this offer. As soon as you do this, JMOL
should start.
Alternatively, you can start JMOL from the command-line. Navigate to the directory containing jmol.jar and issue
the following command:java -jar jmol.jar

The splash screen should appear, and the application should start.

1.11 Installing MUSCLE
We use the sequence alignment tool MUSCLE in one section of the course. This can be installed as follows:

1.11.1 Linux/macOS installation
MUSCLE is available through Bioconda (which should already be set up on your machine):
conda install muscle

1.11.2 Windows installation
At the time of writing, MUSCLE is not available through Bioconda for Windows.
1. Open http://drive5.com/muscle/downloads.htm with your web browser.
2. Download the latest Windows Intel i86 binary, currently muscle3.8.31_i86win32.exe. This will beplaced in your Downloads directory.
3. In git bash change to your home directory with the command cd.
4. Create a new directory called bin with the command mkdir bin.
5. Copy the MUSCLE program to this new directory with the command cp Downloads/muscle3.8.
31_i86win32.exe bin/muscle.exe. This creates a new command called muscle which runs thealignment program.
6. Test that the program can be run by executing the command muscle in the terminal.
In total, the sequence of commands will be:$ cd$ mkdir bin$ cp Downloads/muscle3.8.31_i86win32.exe bin/muscle.exe$ muscle

Note: The $ sign should not be typed - this indicates the command prompt you will see on your screen.

1.11. Installing MUSCLE

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IBioIC Introduction to Bioinformatics Documentation, Release1.12 Installing VirtualBox
VirtualBox is a free-to-use general-purpose full virtualizer for x86 hardware, capable of running a virtual machine foruse by students on the course.
• Download page
Download the appropriate binaries for your system, and follow the installation instructions.

1.12.1 macOS installation
The installer downloads as a .dmg file.
1. Double-click on the .dmg file to open it. This will open a new Finder window showing the file contents.
2. Double-click on the VirtualBox.pkg package, and follow the instructions• Click Continue to run the package to determine if the software can be installed• Click Continue• Click Install to select the standard installation location (you will be prompted for your password)• The installation should report success. Click Close to end.

1.12.2 Linux installation
Follow the instructions for your distribution at the page below:• Installing VirtualBox on Linux

1.12.3 Windows installation
Download and run the installer package from the page below:• VirtualBox downloads

1.13 Indices and tables• genindex• search

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