User manual for
Shikata Laboratory,
Department of Animal Resources,
Advanced Science Research Center,
Okayama University!
The edition of 26th July, 2019

Registration:✤ Before you use the facility, you have to:- Attend the lecture “the lecture for researchers and students,
who use the (laboratory) animals for research and educationin Okayama University”- Attend the lecture on how-to-use the facility- Submit the user registration form to the facility✤ After completion, visit the reception (on the 2nd ﬂoor) andregister your ﬁnger vein pattern to the authentication system for the facility.

!

Authentication:✤ We monitor and record logs of entrance and exit and each ﬂoor.
✤ Authenticate and go through the entrance

one by one.
✤ Un registered person is not allowed to enter

the facility.

How to carry animals into
the facility or the laboratory:✤ Submit the application form forcarrying/buying animals by email.
You can download the form fromthe website of the facility.
✤ Fill in the number of the approved

animal experiment protocol.
✤ The quarantine is required when you

adopt mice/rats from other facilities,except from Charles River, CLEA,and SLC.
✤ Animal must be ordered by thedepartment of animal resources,not by users.

How to take animals out:
✤ Do not let animals escape.
✤ Hide animals with a cover.
✤ Once you take animals out, you cannot take the animals back to the facility.
✤ You can move animals within the facility if needed for experiments.

But it is prohibited to move animals from 4th to 5th ﬂoor, or vice versa.
Moving animals from the infectious experiment area to the main building isalso prohibited.
Please consult the staﬀ when you move animals.
✤ Put animal corpses in the freezer, located on the ﬂoor.

Do not bring carcasses from outside of the facility.
✤ Submit the application form, when you euthanize animals or bring animals

to your laboratory.

How to use experimental rooms:✤ Reserve the room online before use.
✤ When you use the room with special equipments, such as x-ray irradiatorand in vivo imaging system (IVIS), submit the application form to thefacility, thereby you can receive the key of the room.

Bringing equipments:✤ When you bring machines and/or equipments into the experimental/

breeding room, you have to notice to the facility in advance.
✤ As a rule, please take machines/equipments into the facility and bring themout from the facility every time. If you want to keep machines/equipmentsin the facility, please ask the staﬀ in detail.
✤ Machines/equipments must be sterilized/disinfected.

You may ask the facility staﬀ to sterilize your equipments.

The order of ﬂoors to enter in:✤ To prevent animal infection with pathogenic microbes, you must keep theline of ﬂow in the facility.
✤ You cannot enter the 4th and 5th ﬂoors on the same day.
✤ It is better not to use animals and/or enter animal experimental rooms inyour laboratory, before entering the facility on the same day.
✤ You may be penalised if you violate the rules.

GOODNO GOOD

animal experiment rooms
outside of the facility

Use of gene modiﬁed animals:✤ If the animal use protocol corresponds to recombinant DNA experiment,

the experimenter of the animal experiment protocol must be
the experimenter of the approved recombinant DNA experiment protocol.
✤ Use the blue tag for gene modiﬁed mice/rats.

The tag for wildtype is white.

Prohibited:✤ Smoking✤ Eating and drinking except for the special place✤ Bringing in irrelevant things✤ Bringing in irrelevant people…and so on

Contact information:
Shikata Laboratory,
Department of Animal Resources,
Advanced Science Research Center, Okayama University
URL of the facility:
http://www.cc.okayama-u.ac.jp/~animal/
URL of the Institutional Animal Care and Use Committee:
http://www.cc.okayama-u.ac.jp/~animal/committee.html
E-mail:animal@md.okayama-u.ac.jp
Phone number:
086-235-7445（Extension：Shikata 7445）