KAMIYA BIOMEDICAL COMPANY
Rev. 030518.

PRODUCT DATA SHEET
Product: Anti-Thymine Dimer mAb, clone KTM53
Cat. No.: MC-062 (100 g)

Example Data:

Synonyms:

Immunohistochemical staining on untreated human skin.

Cyclobutane pyrimidine dimer (CPD)

Specificity:
Reacts specifically with thymine dimers produced by
UV irradiation in double- or single-stranded DNA.
Does not react with (6-4) photo products.

Ig Isotype:
Mouse IgG1

Immunogen:UV-irradiated salmon sperm DNA

Hybridoma:
Mouse myeloma (P3/X63-Ag8) x immunized mouse(Balb/c) splenocytes.

Format:200 µL of 0.5 mg/mL purified monoclonal antibody in
PBS with protein stabilizer and 0.1% sodium azide.
Purified by Protein A chromatography.

Immunohistochemical staining on UVB-irradiated humanskin.

Storage:

Store at 4°C for short term, store at –20°C for longterm. Avoid repeat freeze/thaw cycles.

Applications and Suggested Dilutions:
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In situ hybridization: Use at a 1:40 to 1:80
dilution. The dilution factor depends on theapplication. Frozen sections of fixed tissue orformalin-fixed, paraffin-embedded sections arerecommended. (Published by end-users.)
DNA blotting: For the detection of thymine
dimers in UV-damaged DNA and thyminedimerized DNA probes. (Published by endusers.)
Immunohistochemistry: Published and confirmed
by KAMIYA BIOMEDICAL COMPANY.
Immunocytochemistry: Published by end-users,
protocol available.
ELISA: Published by end-users, protocolavailable.
Immunoprecipitation: Published by end-users.
Immunofluorescent staining: Published by endusers.

The optimal dilution for a specific application should
be determined by the researcher.

KAMIYA BIOMEDICAL COMPANY, Seattle, WA USA
www.k-assay.com

For in vitro research use only.
diagnostics or in humans.

Not for use inWarranty:
No warranties, expressed or implied, are maderegarding the use of this product.
KAMIYA
BIOMEDICAL COMPANY is not liable for any damage,personal injury, or economic loss caused by thisproduct.

Tel: (206) 575-8068 • Fax: (206) 575-8094
LifeScience@k-assay.com

KAMIYA BIOMEDICAL COMPANY
Rev. 030518.

PRODUCT DATA SHEET
Selected References:

In situ Hybridization
Razzaque, M. et al. In situ localization of Type III and Type IV Collagen-expressing cells in humandiabetic nephropathy. Journal of Pathology, 174: 131-138 (1994)
DNA Blotting
Eller MS. et al. Enhancement of DNA repair in human skin cells by thymidine dinucleotides: Evidence fora p53-mediated mammalian SOS response. Proc. Natl. Acad. Sci USA 94: 12627-12632 (1997)
Goukassian D. et al. FASEB J. DNA oligonucleotide treatment corrects the age associated decline in
DNA repair capacity. 16: 754-756 (2002)
Belanger, F. et al. Mutations in replicative stress response pathways are associated with S phase-specificdefects in nucleotide excision repair. Journal Biological Chemistry. 291(2): 522-537 (January 8, 2016)
6Xiang, Y. et al. m A RNA methylation regulates the UV-induced DNA damage response. Nature.
543(7646): 573-576 (March 23, 2017)
Immunohistochemistry / Immunocytochemistry
Katiyar S. et al. Kinetics of UV light-induced cyclobutane pyrimidine dimers in human skin in vivo: An
immunohistochemical analysis of both epidermis and dermis. Photochemistry and Photobiology 72: 788-793(2000)
Peccia J. et al. Rapid immunoassays for detection of UV-induced cyclobutane pyrimidine dimmers inwhole bacterial cells. Applied and Environmental Microbiology 68: 2542-2549 (2002)
Rubbi CP. and Milner J. P53 is a chromatin accessibility factor for nucleotide excision repair of DNA
damage. EMBO J 22: 975-86 (2003)
ELISA
Berardesca, E. et al. Reduced ultraviolet-induced DNA damage and apoptosis in human skin with topicalapplication of a photolyase-containing DNA repair enzyme cream: Clue to skin cancer prevention. MolecularMedicine Reports. 5: 570-574 (2012)
ImmunoprecipitationHu, J. et al. Genome-wide analysis of human global and transcription-coupled excision repair of UVdamage at single-nucleotide resolution. Genes & Development. 29: 948-960 (May 2015)
Immunofluorescent Staining
Rubbi, C. and Jo Milner. p53 is a chromatin accessibility factor for nucleotide excision repair of DNA
damage. The EMBO Journal. 22(4): 975-986 (2003)
Cruz, GMS. Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on
factor assemblies at DNA damage sites. Nucleic Acids Research. 44(3) (2016)

KAMIYA BIOMEDICAL COMPANY, Seattle, WA USA
www.k-assay.com

Tel: (206) 575-8068 • Fax: (206) 575-8094
LifeScience@k-assay.com