AMMRA
Annual Report
2013-2014

Table of Contents
AMMRA, the Past and Future ............................................................... 1
Welcome Address ................................................................................ 4
AMMRA Members ............................................................................... 6
AMMRA Charter .................................................................................. 7
AMMRA Organizations ....................................................................... 11
Briefing from Member Institutions ..................................................... 13
Australian Phenomics Facility, The John Curtin School of Medical Research,
Australian National University, Canberra, ACT, Australia ........................................... 15
Biological Resource Centre, A-STAR, Singapore ......................................................... 21
Center for Animal Resources and Development, Kumamoto .................................... 25
Developmental Biology and Molecular Medicine, Shanghai ...................................... 31
Korea Research Institute of Bioscience and Biotechnology, Ochang ......................... 35
National Laboratory Animal Center, National Applied ResearchLaboratories,Taipei ..................................................................................................... 41
National Resource Center of Mutant Mice (NRCMM), Nanjing ................................. 47
RIKEN BioResource Center (BRC), Tsukuba................................................................. 55

In Memoriam, Dr. Kazuo Moriwaki .................................................... 63
AMMRA Annual Meetings .................................................................. 69
Contact List ........................................................................................ 71

AMMRA, the Past and Future
President – Dr. Xiang Gao
AMMRA services as a strong bondage for us all.
Personally, I wish to express my appreciation to Dr.
Ken-ichi Yamamura for his vision and tremendous
efforts over last 6 years. Of course I am also in debt
to all members in AMMRA for the achievement of
AMMRA. Nothing can be achieved without your hard
work and enthusiasm. And with your continuous
support, AMMRA will overcome all difficulties.

Aiming the functional annotation of all mammal coding genes, several key institutes
for mouse genetic research in EU, American and Canada formed the International
Mouse Knockout Consortium (IKMC) in 2006. Unfortunately, Asian countries were left
out of circle. This fact prompts us to consider establish a multinational organization
for promoting the mouse mutagenesis in Asia. In November 22, 2006, Shanghai
Institute of Life Science of Chinese Academy organized a special workshop forlaunching the Asian Mouse Mutagenesis and Resource Association (AMMRA) at
Shanghai. More than 40 scientists from China, Japan, Korea, Taiwan, and Singapore
attended this meeting and agreed on the needs for establishing AMMRA. AMMRA
mission is “to promote mouse mutagenesis projects and to facilitate access to mouse
resources in Asia”. The founding members include following institutes: 1) Center for
Animal Resources and Development, Kumamoto; 2) Biological Resource Center,
Singapore; 3) National Laboratory Animal Center, National Applied ResearchLaboratories, Taipei; 4) National Resource Center for Mutant Mice, Nanjing University,
Nanjing; 5) Shanghai Institute of Biological Sciences, Shanghai; 6) Shanghai ResearchCenter for Model Organisms, Shanghai; 7) Peking University- BLARC, Beijing, 8)
Beijing Institute of Laboratory Animal Science, CAMS, PUC, Beijing; 9) Bio-Evaluation
1

Center, KRIBB, Daejeon; 10) Riken BioResource Center, Tsukuba. Most of these
institutes are key resource centers in the region. Dr. Kenichi Yamamura from
Kumamoto University, the chairman of the workshop, was elected as first presidentof AMMRA.
Subsequently, AMMRA annual meeting was held at NRCMM at Nanjing in 2007,
KRIBB at Daejeon in 2008, CARD at Kumamoto in 2009, NLAC NARLabs at Taipei in
2010, BRC at Singapore in 2011, and NRCMM at Nanjing in 2012. At early years,
AMMRA activities were mostly focused on the communication among the resource
centers. Eventually, these resource centers started to hold joined the education
program on mouse related technologies. For instance, CARD had held training classes
for cryopreservation in Shanghai and Beijing in 2009 and 2012. The mouse pathology
workshop held by NLAC NARLabs and Johns Hopkins University was opened to all
AMMRA members in 2010. Moreover, MARC of Nanjing University and BRC RIKEN
agreed to establish a joined international short course series for mouse research.
This short course covers broad range of content in mouse genetics, from history ofmouse research to genetic manipulation, disease model phenotyping, stem cell andcloning, cryopreservation, mouse colonial management. The first class was held in
BRC RIKEN in July 2012. And the second class is scheduled in MARC, Nanjing in July
2013.
To further enhance the solid collaboration for generating, preserving and distributing
mouse resources among members, AMMRA outlined the policies and charters of theassociation, such as the definition, mission, goal, purpose, structure, and governanceat the 5th conference in Taipei in November 2010. At the 6th conference organized in
Singapore on November 2011, IT committee (Dr. Masuya at RIKEN BRC serves as thechair) and annual report committee (Dr. Wang from NLAC NARLabs in Taipei serves as
the chair) were formalized. Currently, an integrated database of mouse strains in
Asian is under construction and the first annual report of AMMRA is published in
2013.
The AMMRA activity also facilitated the birth of Asian Mouse Phenotyping
Consortium (AMPC). In September 23rd 2011, the International Mouse Phenotyping
Consortium (IMPC) kicked off at NIH. The ambitious ten years project is aimed tosystematically phenotype KO strains generated using ES cell lines developed by IKMC.
2

Both RIKEN BRC in Japan and MARC in China became the IMPC member in 2011. In
summer of 2012, Korea also joined the IMPC and NLAC in Taiwan will follow soon. In
accordance with the IMPC pipeline, AMMRA members began to discuss additional
functional screening for better utilizing the existing phenotyping platform in China,
Japan, Korea and Taiwan. These discussions eventually led to the inauguration of the
AMPC at Taipei in 2010. All large phenotyping centers, including NLAC NARLabs in
Taiwan, MARC/NRCMM in China, IDB of Fudan University in China, KRIBB in Korea,
and RIKEN BRC in Japan are founding members of AMPC. In March 2012, AMMRA
and AMPC held joined annual meeting in Nanjing, attracted more than 100
participants.
I believe the most important question is whether AMMRA can continuously fulfill its
role in future. With the new genome manipulation technologies, such as ZFN, TALEN
and CRISPR-Cas, mutant mice are generated much easily today. How do we get thesestrains’ information more accurate and faster? Where can we find better service
center for making these disease models? We are still not even close to satisfy
ourselves.
Another crucial issue for AMMRA is that how AMMRA will take concerted action with
the IMPC. The current IMPC pipeline is focused on only gene functional annotation,
not clinic or pharmaceutical applications. For instance, the challenge model or aging
model is not included in the standard IMPC assay. Nevertheless, these assays may
post the most crucial clinic application. Should the AMPC and AMMRA work together
to solve the problem and initiate the new pipeline?
And at last, the training issue. Currently, most the training courses are provided andmanaged by individual institute, is it possible AMMRA serves as a coordinator for
promoting these courses as well as make them more suitable for systematic studies?
Indeed the earth is becoming flat. And we are all become close friends thorough the
AMMRA activities. This annual report just signatures the new beginning of bright
future of Asian mouse research.

3

Welcome Address
Honorary Former Chair – Dr. Ken-ichi Yamamura
Dear AMMRA members,
AMMRA started in 2006. Since then, there weredramatic changes in terms of production,
cryopreservation, supply and phenotyping ofgenetically engineered mice. To promote life sciencesin Asia, it is essential to trust, help, and cooperate witheach other. I believe that initial phase is successful
through the activities of AMMRA. Let’s move on to thegrowth phase of AMMRA.

4

Welcome Address
Vice President – Dr. Yuichi Obata
The mouse is the most sophisticated experimental
animal among many species. By the scientificendeavors over 100 years, many inbred mouse strains
have been established, DNA sequence of a wholegenome was determined and the methods formodification of genes and manipulation of embryo
have been developed. The disadvantage in the mouseas an experimental animal is its high cost of rearing.
Due to this disadvantage, the science using the mouse
was much delayed in Asia when compared with that inNorth America and Europe. However, in the last decade or so, significant social andscientific advancement and improvement were made in Asia. It is time for Asian
scientists to be in the front line of science. To accelerate this movement in Asia,
AMMRA was established.
There are many issues to be solved for AMMRA. Examples are how to ensure easy
access to mouse strains held by AMMRA members, improve technologies, harmonize
quality control of mouse strains, and train and educate of staff in the member
institution. It would be very difficult for a single institution to solve all these issues,
but it may be possible as an association if there are collaborative efforts among
member institutions.
As a vice president of this association, I do my best to help the AMMRA president, Dr.
Gao and each member institutes to accomplish the common goals of AMMRA bysolving issues stated above.

5

AMMRA Members●

Australian Phenomics Facility, Australian National University, Canberra★

Biological Resource Center, Singapore★

Center for Animal Resources and Development, Kumamoto☆

Institute of Developmental Biology and Molecular Medicine of Fudan
University, Shanghai★

Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences,
Peking Union Medical College, Beijing★

Laboratory Animal Resource Center, KRIBB, Ochang★

Laboratory of Cardiovascular Genomics of Ewha Woman’s University, Seoul
Nanfang Center for Model Organism, Shanghai☆

National Institute of Food and Drug Safety Evaluation, Seoul★

National Laboratory Animal Center, National Applied Research Laboratories,
Taipei★

National Resource Center for Mutant Mice, Nanjing University, Nanjing★

Peking University-Beijing Laboratory Animal Research Center Mouse
Genomics Center, Beijing★

Riken BioResource Center, Tsukuba☆

Seoul National University, Seoul★

Shanghai Laboratory Animals Center, Shanghai Institute of Biological Sciences,
Shanghai☆

Sookmyung Women’s University, Seoul☆

Yonsei University, Seoul★ Founding members● Newly joined members (2013)

6

AMMRA Charter

Definition
The Asian Mouse Mutagenesis and Resource Association (AMMRA) is a collaborative
group of the development, archiving/distribution, coordination of phenotyping andinformatics of mutant mice in Asia.
MissionTo promote and coordinate the development, archiving/distribution, phenotyping,and informatics of mutant mouse, and to facilitate access to mouse resources in Asia
Goal
Use of mouse models for understanding the genome function and improvement of
human healthPurpose
1. AMMRA is an international organization whose members, in a manner consistent
with the governmental obligations and legal responsibilities of each, pledge to:(1) facilitate the use of mouse resource and research centers across nationalboundaries and barriers in Asia;(2) commit to cooperative, standardized approaches to the development,archiving/distribution and quality control of mouse models;(3) establish commonly shared principles on operation control as regards toanimal health, genetics and environmental management;(4) work cooperatively to facilitate access to and utilization of available resources,
services, and expertise in all aspects of the development,archiving/distribution, coordination of phenotyping and informatics of mousemodels2. AMMRA’s goal is to facilitate the use of mouse models of human disease, behavior
and development for the benefit of researchers in biomedical field.
Structure and Governance
1. The primary criterion for admission to AMMRA institutional membership is each7

member must be a mouse repository or mouse research facilities, supported by
the national government, which has an independent, sustainable operating
budget, and dedicated infrastructure and resources. The applicant organizationmust also agree to the AMMRA’s principles of operations, and actively participate
in AMMRA operations. Commercial membership is also available based on the
merits of the applicant company in compliance to the AMMRA’s principles andwith support to AMMRA activities.
2. AMMRA is governed by the members of Board of Directors (BOD) includingpresident and vice president, comprised of the institutional members of each
Asian country / region.
3. The Board of Directors is responsible for developing strategic and financial plansand, when appropriate, will appoint committees to address specific goals. It is
responsible for:(1) convening at least one meeting annually(2) electing officers(3) considering additions and eliminations of the membership(4) ensuring to maintain the web site(5) empowering educational activities(6) adopting the standards of operation agreed upon in committee(7) preparing amendments to the AMMRA Charter(8) fund raising with governmental, industrial and private funding agencies andacademic institutions(9) overseeing the ultimate dissolution of AMMRA
4. President of AMMRA is elected by the members of Board of Directors (BOD). The
term of President is 2 years and takes turns to be selected from different area.
5. The Vice-President is nominated by the president. The President andVice-President are responsible for the activities and finances of AMMRA, with theconcurrence of the BOD.
6. Prospective member organizations must make application through the BOD of therelevant area. The Vice-President will assist in preparing the case for admission,bring it to the convened AMMRA Board, and shepherd it through to a vote.
Elimination of a member organization will proceed in a similar fashion with the
BOD of the relevant area moving for elimination.
7. Although consensus on all matters will be sought, practice dictates passage of all
votes by a half majority, provided a quorum is present.
8

Head office
President’s institution
Websitehttp://www.ammra.info/
Membership
1. Institutional membership:
Annual fee: $1,000 USD
Duty:(1) Providing information on their resources(2) Sharing mice resources(3) Support to expanding professional techniques in mouse biology(4) Providing annual report according to AMMRA forms
2. Individual membershipOpen to mouse scientists in Asia
Integration of AMMRA into IKMCAMMRA as a whole is integrated into IKMC
Terms of Reference
The role of the Steering Committee is to provide oversight of and to facilitate
coordination between international efforts in the generation of knockout mouse
resources. Specifically the Steering Committee will:
1. Promote coordination of the international efforts through the sharing ofproduction plans (such as gene lists, plans for mouse production, etc.) andproduction status to minimize unnecessary redundancy;
2. Promote maximum efficiency in the generation of mouse-gene knockouts byensuring the sharing of information regarding new approaches and technologies
developed during the programs;
3. Ensure free and open release of data and resources generated;
4. Promote dissemination of knowledge, tools, policy and best practice in the field ofmouse genomics;
5. Promote coordination on issues such as archiving and distribution to ensure thedata and resources generated are readily accessible to the scientific community;
6. Promote discussion of future strategy for mouse functional genomics;
9

7. Coordinate public communications regarding individual efforts or the international
effort as a whole.
Advisory Board
Advisory Board members will be invited from global society in the field of mouse
genetics.
.

10

AMMRA Organizations

President(Term 2012-2014)
Vice-President(Term 2012-2014)
BOD from Institutional Members

Xiang Gao, NRCMM
Yuichi Obata, Riken BRC
Kumamoto University
Riken BRC
Nanjing University
KRIBB
BRC
NLAC NARLabs

General Secretary and Treasurer

Je Kyung Seong, SNU

IT Committee

Hiroshi Masuya, Riken BRC (Chair)
Arun Kumar Manickam, BRC
Ki-Hoan Nam, KRIBB
Xiaojun Lei, NRCMM
Hsian-Jean Genie Chin, NLAC NARLabs
Yukiko Yamazaki, NIG

Annual Report Committee

Chi-Kuang Leo Wang, NLAC NARLabs(Chair)11

12

Briefing from Member Institutions

13

14

APF

Australian Phenomics Facility
The John Curtin School of Medical Research
Australian National University, Canberra, ACT, Australia
Institute Director/ President

Dr. Steve Winslade, CEO
Dr. Chris Goodnow, CSO

AMMRA contact person

Dr. Edward BertramPhone+61-2-6125-1328

E-mail

Edward.Bertram@anu.edu.au

Institute address

Australian Phenomics Facility, The Australian
National University, Hugh Ennor Building 117,
Garran Road, Canberra ACT, Australia 2601

Websitewww.apf.edu.au15

APF, The John Curtin School of Medical Research, Australian National
University, Canberra, ACT, Australia
Part I – Institution Briefing

Brief Description
The Australian Phenomics Facility (http://www.apf.edu.au/ ) is non-profit andsupports open access large-scale phenotyping of humans and mice to uncover the
biological drivers in human disease. The Australian Phenomics Facility specialises inthe development, characterising and archiving of mouse models of human disease.
We have an experienced genomics and bioinformatics capability focused on theidentification of single nucleotide polymorphisms and the phenotyping capability tomake the biological associations with probable human disease traits. Our goals are to
firstly derive the underlying genetic mechanisms, and then look to extend this acrossthe population and better understand cohort differences and responses.
The facility was established in 2004 and receives funding from the AustralianGovernment’s NCRIS, Super Science and CRIS programmes through the Australian
Phenomics Network and contributions from the Australian National University. We
have an open access policy and support academic and corporate research
programmes in Australia and internationally.
Accreditation: AEEC, AALAS, IACUC, OGTR, NLRD,
Memberships: APN, IMPC, ISTT, AMPC,Resources:
All services are publically accessible on partial to full cost recovery.
Animal Holding: The APF offers a complete strain management package to meet
researchers needs. From the moment of importing, rederiving or creating your strain,a project manager and highly trained technical staff will care for and takeresponsibility of all your mouse maintenance needs.
Australian Phenome Bank: (http://pb.apf.edu.au ). The Australian Phenome Bank
(APB) is a centralised repository of genetically modified mouse strains and strain
information used in Medical Research in Australia. The database includes gene andallele information, descriptions of phenotypes of both homozygous and heterozygous16animals and links to publicly available information relating to each strain. The Curator
reviews all strain submissions, ensures correct nomenclature is assigned and registers
alleles and strains with MGI.
Missense Mutation Library: (https://databases.apf.edu.au/mutations/ ). Utilisingwhole exome sequencing and our N-ethyl-N-nitrosourea (ENU) mouse mutagenesis
pipeline we are generating hundreds of thousands of known missense and nonsense
variants on the inbred C57BL/6 genetic background. We are rapidly expanding thenumber of genes with mutations and in a large proportion of genes we havecollected an allelic series. Interrogation of the Missense Mutation Library is assisted
through annotations by Mouse Genome Informatics (MGI) and Online Mendelian
Inheritance in Man (OMIM) and Orphanet, linking your search to publically available
information related to your gene of interest.
Cryopreservation/ IVF / Rederivation: The APF offers the latest techniques forprotecting and recovering your mouse strain through the manipulation of gametes.
Genomics: Next generation sequencing technology and bioinformatics has opened
up a new dimension for integrating phenomics, molecular biology, and systems
biology for mouse and human. Our highly validated pipeline will accurately identify
your variants and we provide a number of phenotyping assays.
Phenotyping: The APF’s Scientific Programs Team combines scientific expertise,
project management as well as laboratory-based expertise to undertake complex
research investigations for the research community. Our team regularly manage theproduction of gene variant mice, coordinate and perform phenotyping assays andprovide a range of data management and analysis expertise and services. We have arange of equipment including Mesoscale discovery S6000, Indiko Plus Clinical
Chemistry, small animal bioluminescence, fluorescence, micro CT, histology, PIXImus
densitometry.

17Part II - Mutant Rodent Resource
Australian Phenome Bank
Repository Director
Repository Director’s Email
Numbers of repository service related
personnel?

Stuart Read
Stuart.Read@anu.edu.au
PI: 1
Staff: 4
Live: 700

Mouse strain resource

Cryopreserved: 2388
ENU induced mutations/ MML: 175,857
Live: 1

Rat strain Resource
IMSR registered?
Source of resource

○ Yes
● No
○ Government funding generated○ Deposited by researchers

Geneticallymodified, 1722,
72%

Mutant
Mouse
Strains
Gene trap, 37,2%
Wild type andspontaneous
mutation, 121,5%

Non-targeted,inducedmutation, 508,21%

18

Part III - Special Announcement(For posting events from the past year or which will happen in the coming year)
EVENT
Towards precision medicine: Phenotyping human diseases in mice
The 16th Frank and Bobbie Fenner Conference
ANU, Canberra, 20-23 October 2014
http://towardsprecisionmedicine-symposium2014.org.au/
Including

2 day Symposium
AMMRA-AMPC annual business meeting
1.5 day AMMR-AMPC Workshop

19

20

Biological Resource Centre, A-STAR, Singapore
Institute Director/ President

Dr. Ian Gray

Scientific Director

Dr. Sathivel Ponniah

AMMRA contact person

Manickam Arun Kumar

Phone+65-6464-2325

E-mailarunkumar@brc.a-star.edu.sg

Institute Address

8A Biomedical Grove,
BRC Dept. 3 Level 7 Immunos Building
Singapore 138668

Websitehttps://www.brc.a-star.edu.sg/

21

Biological Resource Centre, A-STAR, Singapore
Part I – Institution Briefing
BRC, which started its operations in 2005, is part of the Biomedical Sciences
Institutes which comes under the Agency for Science, Technology and Research. It isa non-profit entity and is funded by A*STAR.
It first obtained AAALAC accreditation in 2009 and has been AAALAC accredited sincethen. As a service facility we provide husbandry and other services to our users who
can be from academic or commercial entities. The services we carry out are listed
below and although we carry out cryopreservation of strains developed here at the
BRC, we do not consider this as a repository for strains that are generated outside of
BRC. We do not at the moment have a dedicated phenotyping analysis platform, butsome of the users have specific phenotyping equipment that they use for their own
research. These are accessible to other researchers through collaborations.
Scientific Services available at the Biological Resource Centre at Biopolis.
• Re-derivation by embryo transfer.
•

Micro-injection – DNA or RNA into fertilized eggs and ES cell injections into
blastocyst and subsequent implantation and generation of founder transgenic
or chimeric mouse respectively.

•

Polyclonal antibody production in mice and rabbits.

•

Cryopreservation of mouse sperm and embryo is available as a service.

•

IVF to generate animals from frozen sperm or implantation of frozen embryos
to generate mouse lines.

Animal Gene Editing Laboratory (AGEL) Targeting vectors: Design and construction of any gene targeting(knockout/knockin) and transgene expression vectors.
 Targeted ES lines and Kockout/Knockin mice: Generation of
germline-competent targeted ES cell lines, and subsequent knockout/knockinmice by blastocyst injection.
 Transgenic mice: Creation of Rosa26 locus-based (non-random integration)transgene expression mice with Cre-activated tissue specificity.

22 Tetracycline inducible models: Creation of tetracycline-inducible mice with single copy integration of transgene at a specific genomic locus(non-random integration).
 Nuclease-based gene editing in cells and animals: Construction and activity
testing of TAL Effector Nucleases (TALENs) targeting to specific genomic regionof any species.
Other services: Establishing ES cell lines from mice.
 Supplying of mouse embryonic fibroblast.
 Genotyping of mice.
 Colony maintenance and breeding set ups.
 Dosing of rodents.
 Toxicology studies in rodents.
 Implantation of cell lines or primary tumours into immunocompromised mice.

23

24

Center for Animal Resources and Development, Kumamoto
Institute Director/ President

Yuichi OikeAMMRA contact person

Naomi Nakagata

Phone+81-96-373-6564

E-mailnakagata@kumamoto-u.ac.jp

Institute address

2-2-1 Honjo, Kumamoto 860-0811, Japan

Websitehttp://card.medic.kumamoto25

Center for Animal Resources and Development, Kumamoto

Part I – Institution Briefing
 CARD is located in the Honjo campus of Kumamoto University, agovernment-funded non-profit national university corporation. It was establishedin 1998 in line with recommendations made in “Preservation, Supply andDevelopment of Genetically Engineered Animals”, a report published by theJapanese Ministry of Education, Culture, Sports, Science and Technology.
 Further details of CARD and its work can be found on the CARD website at
http://card.medic.kumamoto-u.ac.jp/card/english/index.html.
 CARD is a global hub for the production, phenotyping, cryopreservation, andsupply of genetically engineered mice. To promote biological sciences worldwide,
CARD produces genetically engineered mice and exchangeable gene trap ES cellclones; cryopreserves mouse embryos and sperm; supplies these resources; andorganizes training courses to educate people. To this end, Kumamoto Mouse Clinic
commenced full activities in April 2013.
 As a founding member of the International Gene Trap Consortium, the Federationof International Mouse Resources (FIMRe), and the Asian Mouse Mutagenesis andResource Association (AMMRA), CARD continues to contribute to the promotionof biological sciences worldwide. CARD transfers data on mouse strains to theInternational Mouse Strain Resource (IMSR, http://www.findmice.org/ ) and dataon Exchangeable Gene Trap Clones (http://egtc.jp/ ) to the International Gene
Trap Consortium (IGTC, http://www.genetrap.org/index.html ).
 For information regarding our various services, please visit the CARD website at
http://cardb.cc.kumamoto-u.ac.jp/transgenic/index.jsp.

26

Part II - Mouse/Rat Strain Resource
Center for Animal Resources and Development
CARD, Kumamoto University
Repository Director

Naomi Nakagata

Emailnakagata@kumamoto-u.ac.jp

Repository personnel

PI: 1
Staff: 10

Mouse strain resource

Cryopreserved 1,635
ES cells
1,266

IMSR registered?

● Yes
○ No

Source of resource○ Government funding generated● Deposited by researchers

Wild type andspontaneous
mutation, 16, 1%
Non-targeted,inducedmutation, 5, 0%

Mutant
Mouse
Strains
Geneticallymodified, 691,
24%

27

Gene trap, 2,142,
75%

Part III - Special Announcement
We held overseas training courses on the Cryopreservation of Mouse Germplasm at
the venues below:
1) Shanghai Laboratory Animal Center, Chinese Academy of Sciences, Shanghai(2002)
http://card.medic.kumamoto-u.ac.jp/card/japanese/kenkyu/sigen/kensyu/kensyukokugai.html
2) College of Life Sciences, Peking University, Beijing (2005)
http://card.medic.kumamoto-u.ac.jp/card/japanese/kenkyu/sigen/kensyu/kensyupekin.html
3) Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), Chungbuk (2008)
http://card.medic.kumamoto-u.ac.jp/card/japanese/kenkyu/sigen/kensyu/kensyuKorea.html
4) BIOPOLIS, Singapore (2011)
http://card.medic.kumamoto-u.ac.jp/card/japanese/kenkyu/sigen/kensyu/kensyu
23Singapore.html
5) National Laboratory Animal Center, National Applied Research Laboratories,
Taipei (2012).
http://card.medic.kumamoto-u.ac.jp/card/japanese/kenkyu/sigen/kensyu/kensyu_Taiwan.html
6) National Institutes for Food and Drug Control, Beijing (2013)
http://card.medic.kumamoto-u.ac.jp/card/japanese/kenkyu/sigen/kensyu/kensyu_Beijing.html
7) Spanish National Centre for Biotechnology (CNB-CSIC), Madrid (2013)
http://card.medic.kumamoto-u.ac.jp/card/japanese/kenkyu/sigen/kensyu/kensyu_Madrid.html
Recently, we developed the following reproductive engineering techniques in mice:1) Collection and Transport at Cold Temperature of Cauda Epididymis
http://card.medic.kumamoto-u.ac.jp/card/english/sigen/manual/cetransp.html
2) Simple Vitrification of Mouse Oocytes
http://card.medic.kumamoto-u.ac.jp/card/english/sigen/manual/cryooocyte.html

283) Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved
Spermatozoa
http://card.medic.kumamoto-u.ac.jp/card/english/sigen/manual/jaxcryoivf.html

29

30

Developmental Biology and Molecular Medicine, Shanghai
Institute Director/ President

Dr. Tian Xu

AMMRA contact person

Dr. Xiaohui Wu

Phone+86-21-6564-3718

E-mailxiaohui_wu@fudan.edu.cn

Institute address

Yifu Building of Science and Technology, Fudan
University, Shanghai, 200433, China

Websitehttp://idm.fudan.edu.cn

31Developmental Biology and Molecular Medicine, ShanghaiPart I – Institution Briefing
The Institute of Developmental Biology and Molecular
Medicine at Fudan University (IDM) is a non-profit
biomedical research center and the host institute of China
National Center for International Research of Development and Disease. IDM pursues
broad biomedical researches with a strong effort in the area of developmental
biology and molecular mechanisms of human diseases. It works closely with the
School of Life Science, Fudan University for in building scientific and academic
excellence in China. In addition to the funding from Fudan University, the research
activities of IDM are also supported by government funding agencies including the
Ministry of Science and Technology (MOST), the National Natural Science Foundationof China (NSFC), and the Sciences and Technology Commission of Shanghai
Municipality (STCSM).
IDM holds a SPF mouse facility accredited by STCSM. It not only act as the repositoryof PBmice, a collection of 5,000 live or cryopreserved mutants generated by piggyBac
insertional mutagenesis (http://idm.fudan.edu.cn/PBmice ), but also provides paid
service for GM mouse generation, cryopreservation, and various phenotyping assays.
The facility is expected to be a loyal partner in projects initiated by IDM researchersand external users who seek to understand the mechanisms of disease anddevelopment. It provides expertise, equipment and other resources to effectively
move individual projects forward in a timely manner.

32

Part II - Mouse/Rat Strain Resource

PBmice database, Shanghai
Repository Director

Dr. Tian Xu

EmailTian.xu@yale.edu

Repository personnel

PI: 6
Staff: 30

Mouse strain resource

Live Strains

Mice:1408/ Rat:16

Cryopreserved
ES cells
VectorMice:4,511
Mice:11
Mice:14 /Rat:3

IMSR registered?

○ Yes
● No

Source of resource● Government funding generated● Deposited by researchers

Non-targeted,inducedmutation, 5, 0%Gene trap,
2,142, 75%

Mutant
Mouse
Strains
Wild type andspontaneous
mutation, 5, 0%

Geneticallymodified, 691,
24%

33

34

Korea Research Institute of Bioscience and Biotechnology, Ochang
Institute Director/ President

Dr. Hyoung-Chin Kim

AMMRA contact person
Phone

Dr. Hyoung-Chin Kim+82-43-240-6560

E-mailhckim@kribb.re.kr

Institute address

Laboratory Animal Resource Center, KRIBB,
30 Yeongudanji-ro, Ochang-eup, Chengwon-gun,Cheongju, Chungcheongbuk-do 363-883, Korea

Websitehttp://www.kribb.re.kr/(KRIBB)

http://mouse.kribb.re.kr/(LARC, KRIBB, under reconstruction)

35

Laboratory Animal Resource Center, KRIBB, OchangPart I – Institution Briefing
Korea Research Institute of Bioscience and Biotechnology (KRIBB) is a non-profit
institute funded by Korean government.
The Korea Research Institute of Bioscience and Biotechnology (KRIBB) is thegovernment-funded non-profit research institute dedicated to state-of the-art
bioscience and biotechnology. The Korean government has been systematicallypromoting biotechnology through the national plan [Bio-Vision 2016] to make Korea
much stronger in bioscience and technology.

KRIBB is also designated by Ministry of Science, ICT and Future Planning (MSIP) as aprincipal institution responsible for archiving resources used for researches anddevelopment in the field of bioscience. The Laboratory Animal Resource Center(LARC), KRIBB is one of the centers in KRIBB and is in charge of the archiving mouse
resources.
Therefore, one of the main missions of LARC, KRIBB is to archive the mouse resource.
Deposited mouse resources are kept as frozen embryos or sperms. Those frozen
resources are re-vitalized when requested by researchers. Recently, we have36

established broad-based mouse phenotyping pipeline to help identification of
mammalian gene functions. LARC, KRIBB have a platform to produce mutant mice by
microinjection or RGEN. The information on the deposited mouse strains isaccumulated into our data base which is opened to public in a limited fashion. LARC,KRIBB will provide these services to scientific society, soon.
LARC, KRIBB have been always seeking ways to provide better services to researchcommunity.

37

Part II - Mouse/Rat Strain Resource

LARC
Repository Director

Dr. Hyoung-Chin Kim

Emailhckim@kribb.re.kr

Repository personnel

PI: 4
Staff: 23

Mouse strain Resource

Live: 15
Cryopreserved: 129

IMSR registered?

○ Yes
● No

Source of resource● Government funding generated● Deposited by researchers

Wild type andspontaneous
mutation, 5,3%

Inducedmutation , 23,
16%

Mutant
Mouse
Strains

Gene trap, 10,7%Targeted
mutation andTransgenic,
106, 74%38

Part III - Special Announcement
Laboratory animal Workshop- Basic technics for laboratory animals- Health and genetic monitoring- Embryo freezing/thawing/transfer
Date: November 18-19, 2014
Place: LARC, KRIBB (Ochang campus)
Organizer: LARC, KRIBBCIEA-KRIBB Joint
Workshop- Health monitoring& management
Date: February 13-14, 2014
Place: KRIBB
Organizer: KRIBB-CIEA

39

40

National Laboratory Animal Center,
National Applied Research Laboratories, Taipei
Institute Director/ President

Dr. Chun-Keung Yu

AMMRA contact person

Dr. Chi-Kuang Leo Wang

Phone

+886-2-2789-5567

E-mailcklwang@narlabs.org.tw

Institute address

128 Academia Road, Section 2, Nankang, Taipei
115, Taiwan

Websitehttp://www.nlac.org.tw/english/default.asp

41National Laboratory Animal Center,
National Applied Research Laboratories, Taipei

Part I – Institution Briefing
The National Laboratory Animal Center (NLAC) is a non-profit institute under the
National Applied Research Laboratories (NARLabs); the funding source is supportedby the government. The missions of NLAC NARLabs are to supply rodent resources
and technical supports for the research communities in Taiwan. NLAC NARLabs
operations are accredited by ISO/IEC17025, ISO27001:2005, ISO 9001:2008, andawarded by AAALAC Full Accreditation; NLAC NARLabs services are also enrolled in
Performance Evaluation Program for Diagnostic Laboratories (PEP) to assure quality
control performance.
In addition to the rodent supply and diagnostic services, NLAC NARLabs has been
devoting deeply in the generation, archiving and sharing of GM mouse and rat
models. To advocate the use of important rodent resource in Taiwan, NLAC
NARLabs lunched a regional repository project in 2009, named Rodent Model
Resource Center (RMRC). RMRC-NLAC offers cryopreservation and ART services
upon requests to collect and preserve GM models generated by researchers in
Taiwan. Furthermore, RMRC-NLAC also generates novel GM models to enrich therepository resource. Since 2010, RMRC-NLAC has been a registered member ofInternational Mouse Strain Resource (IMSR). Since 2013, NLAC became a memberof International Mouse Phenotyping Consortium (IMPC). With the successful
development of a unique BAC gene manipulation system, NLAC NARLabs
subsequently strengthens the transgenic mice/rat platform as well as the conditional
knockout / Cre mice production pipelines. Today, NLAC NARL also provide knock-out
and/or knock-in service with latest CRISPR/Cas9 and TALEN approaches.
To better support the research communities, NLAC, NARLabs will continuously seek
for connection and collaboration with renowned organizations and associations
around the world.

42

Part II - Mouse/Rat Strain Resource
Rodent Model Resource Center (RMRC)
Repository Director

Dr. Chi-Kuang Leo Wang

Emailcklwang@narlabs.org.tw
PI: 6

Repository personnel

Staff: 35

Mouse strain Resource

Live: 56
Cryopreserved: 150

Rat strain Resource

Live: 8Cryopreserved: 2

IMSR registered?

● Yes
○ No

Source of resource● Government funding generated● Deposited by researchers

Wild type andspontaneous
mutation, 19,9%Targeted
mutation andTransgenic,
193, 89%

Mutant
Mouse
Strains

Inducedmutation , 4,2%

43Part III - Special Announcement
Local training
Strategies for Modeling Human Disease in the Laboratory Mouse
Date: Mar. 26th- 28th, 2014
Place: Academia Sinica
Organizer: NLAC NARLabs
Co-Organizer: The Jackson Laboratory
Mouse and Rat Blood Sampling, Drug Administration, Euthanasia Practical Training
May 6th , 2014 @ NLAC NARLabs-Taipei Center & Tainan Facility
May 12th , 2014 @ NLAC NARLabs-Taipei Center & Tainan Facility
Introduction of Mice Anatomy and Histology
July 8th ,2014 @ NLAC NARLabs-Taipei Center & Tainan Facilitythth
Aug 5 -12 , 2015 @ NLAC NARLabs-Taipei Center
Human Disease Modeling (TBD)
Date: Sep., 2015
Place: Academia Sinica
Organizer: NLAC NARLabs
Co-Organizer: TBD

44

International Mouse Phenotyping Conference

Introduction of Mice
Anatomy & Histology

Genetically Modified Animal
Care & Refinement Workshop

Strategies for Modeling Human Diseasein the Laboratory Mouse

45

46

National Resource Center of Mutant Mice (NRCMM), Nanjing
Institute Director/ President

Dr. Xiang Gao

AMMRA contact person

Dr. Jing Zhao

Phone

+86-25-5864-1533

E-mailzhaojing@nicemice.cn
gaoxiang@nju.edu.cn

Institute address

12, Xuefu Road, Pukou District, Nanjing
210061

Websitewww.nicemice.cn
www.nbri-nju.com/en-us/

47

National Center for Mutant Mice of China, NanjingPart I – Institution Briefing
National Resource Center of Mutant Mice (NRCMM) is designated by the Ministry of
Science and Technology of China, recognized as one of the major laboratory animal
resource center in China. NRCMM is affiliated with the Model Animal ResearchCenter of Nanjing University (MARC) and Nanjing Biomedical Research Institute ofNanjing University (NBRI). NRCMM has been awarded full accreditation by AAALACI
since 2006.
The mission of NRCMM is to promote the biomedical research in China by providing
mouse models and related to services. NRCMM services include depository,
cryopreservation, and distribution of mutant mice. As one of the largest transgenic
center in China, NRCMM also provide services to generate and phenotype genome
modified mouse lines for biomedical research community. In addition, NRCMM also
hosts education programs for mouse colony management and mouse genetics
studies.
NRCMM has three departments. The resource department is responsible for themouse importation, breeding, distribution, cryopreservation, andgenetic/microbiological monitoring. The technical service team provides noveltransgenic and phenotyping services. And the administration office manages thehuman resource, financial affair, facility maintenance, and education program.
NRCMM joined the International Mouse Phenotyping Consortium in 2011 and is also
the founding member of Asian Mouse Mutagenesis and Resource Association(AMMRA) and Asian Mouse Phenotyping Consortium (AMPC). Since 2011, NRCMM
became a member of International Mouse Strain Resource (IMSR). NRCMM is also
one of the sponsors of China Mouse Strain Resource (CMSR). In 2014, NRCMM holds
total 2185 strains of mice. With the new animal facility brought into service, NRCMM
48

generated over 920 of customized mice and supplied more than 300 strains tocustoms in China. NRCMM also helped the Chinese scientists in 29 research
institutes/universities to import mouse strains from US, UK, France, Australia andAustria. NRCMM is also very grateful to receive more mouse strains donated byscientists in China.
Additional information can be found at www.nicemice.cn andwww.nbri-nju.com/en-us/

49Part II - Mouse/Rat Strain Resource

National Resource Center of Mutant Mice (NRCMM)
Repository Director

Dr. Xiang Gao

Emailgaoxiang@nju.edu.cn

Mouse strain Resource

Live:399
Cryopreserved: 460

IMSR registered?

● Yes
○ No

Source of resource● Government funding generated● Deposited by researchers

Wild type andspontaneous
mutation, 47, 6%

Induced mutation, 77, 9%

Mutant
Mouse
Strains50

KO/KI/transgenic,
731, 85%

Part III - Special Announcement
1.

5th Summer Camp for Model Animal Research. Nanjing, China. July 7-11, 201455 senior college students were selected from 298 applicants from 69
universities.

2.

Nanjing University MARC/RIKEN BRC International Short Summer Course of the
Mouse. Tsukuba, Japan. July 28-30, 2014
The course provided comprehensive introduction for model technology ofmouse genetics and phenotyping.

3.

Nanjing University MARC/RIKEN BRC International Short Summer Course of the
Mouse. Nanjing. July, 2015

4.

1st China Mouse Strain Resource (CMSR) meeting. Nanjing. June 8 2014
There are over 100 members in CMSR, including universities, research institutes,
and biomedical companies. CMSR holds 590 donated strains from different
members.

Selected Publications from NRCMM
1. Huang Z, Ruan H, Zhang Z, Chen W, Lin Z, Zeng H, Gao X (2014) Mutation in thefirst Ig-like domain of Kit leads to JAK2 activation and myeloproliferation in mice.
The American journal of pathology 184: 122-132
2. Zhang Q, Li YF, Zhang L, Yang N, Meng J, Zuo PP, Zhang Y, Chen J, Wang L, Gao X,
Zhu DH (2013) E3 ubiquitin ligase RNF13 involves spatial learning and assembly of
the SNARE complex. Cellular and Molecular Life Sciences 70: 153-165.
3. Huang Z, Ruan H-B, Xian L, Chen W, Jiang S, Song A, Wang Q, Shi P, Gu X, Gao X(2014) The stem cell factor/Kit signalling pathway regulates mitochondrial
function and energy expenditure. Nature communications 5: 4282.
4. Qi X, Xu JY, Gu PY, Yang X, Gao X (2014) PTEN in smooth muscle cells is essential
for colonic immune homeostasis. International Journal of Biochemistry & Cell
Biology 53: 108-114.
5. Wen Luo, Xia Zhao, Hengwei Jin, Lichan Tao, Jingai Zhu, Huijuan Wang, Brian A.
Hemmings, and Zhongzhou Yang. (2014) Akt1 signaling coordinates BMP signaling
and beta-catenin activity to regulate second heart field progenitor development.
Development (Accepted)
6. Xia Zhao, Shuangshuang Lu, Junwei Nie, Xiaoshan Hu, Wen Luo, Xiangqi Wu,
Hailang Liu, Qiuting Feng, Zai Chang, Yaoqiu Liu, Yunshan Cao, Haixiang Sun, Xinli
51

Li, Yali Hu, Zhongzhou Yang. (2014) Phosphoinositide-Dependent Kinase 1 andmTORC2 Synergistically Maintain Postnatal Heart Growth and Heart Function inMice. Mol. Cell. Biol. 34 (11):1966-75. (Spotlight article/cover)
7. Baiyin Yuan, Ping Wan, Dandan Chu, Junwei Nie,Yunshan Cao, Wen Luo,
Shuangshuang Lu, Jiong Chen* and Zhongzhou Yang*. (2014) A
Cardiomyocyte-Specific Wdr1 Knockout Demonstrates Essential Functional Roles
for Actin Disassembly during Myocardial Growth and Maintenance in Mice. Am J
Pathol. 184 (7):1967-80 (*Co-corresponding author)
8. Pei Wang, Beibei Mao, Wen Luo, Bin Wei, Wenjian Jiang, Dong Liu, Lei Song,
Guangju Ji, Zhongzhou Yang,* Yong-Qiang Lai,* Zengqiang Yuan*. (2014) The
alteration of Hippo/YAP signaling in the development of hypertrophic
cardiomyopathy. Basic Res Cardiol.109 (5):435 (*Co-corresponding author).
9. Yijun Gao, Wenjing Zhang, Xiangkun Han, Fuming Li, Xujun Wang, Rui Wang,
Zhaoyuan Fang, Xinyuan Tong, Shun Yao, Fei Li, Yan Feng, Yihua Sun, Yingyong
Hou, Zhongzhou Yang, Kunliang Guan, Haiquan Chen, Lei Zhang & Hongbin Ji.
(2014) YAP inhibits squamous transdifferentiation of Lkb1-deficient lungadenocarcinoma through ZEB2-dependent DNp63 repression. Nat. Commun. 5:4629.
10. ShuilongGuo, Hui Ye, Yan Teng, Youliang Wang, Guan Yang, Xiubin Li, Chong Zhang,
Xue Yang, Zhongzhou Yang and Xiao Yang.(2013)Akt-p53-miR-365-cyclin D1/cdc25A
axis contributes to gastric tumorigenesis induced by PTEN deficiency. Nat.
Commun.4:2544.
11. Xiangqi Wu, Yunshan Cao, JunweiNie, Hailang Liu, Shuangshuang Lu, Xiaoshan Hu,
Jingai Zhu, Xia Zhao, Jiandong Chen, Xiaohu Chen, Zhongzhou Yang* and Xinli Li*.
(2013) Genetic and Pharmacological Inhibition of Rheb1-mTORC1 Signaling Exerts
Cardioprotection against Adverse Cardiac Remodeling in Mice. Am. J. Pathol. 182:
2005-2014. (*co-corresponding author)
12. Jie Yan, Guangsen Shi, Zhihui Zhang, Xi Wu, Zhiwei Liu, Lijuan Xing, Zhipeng Qu,
Zhen Dong, Ling Yang and Ying Xu (2014) An intensity ratio of interlocking loops
regulates circadian period length. Nucleic Acid Research, 42(16):10278-87.
52

13. Zhiwei Liu, Moli Huang, Xi Wu, Guangsen Shi, Lijuan Xing, Zhen Dong, Zhipeng Qu,
Jie Yan, Ling Yang, Satchidananda Panda & Ying Xu (2014）PER1 phosphorylation
specifies feeding rhythm in mice. Cell Report, 7(5) 1509-1520.
14. Xi Wu, Binbin Wang, Zhen Dong, Sirui Zhou, Zhiwei Liu, Guangsen Shi, Yunxia Cao,Ying Xu （2013）A NANOS3 mutation linked to protein degradation causes
premature ovarian insufficiency. Cell Death & Disease, 4:e825.
15. Guangsen Shi, Lijuan Xing, Zhiwei Liu, Zhipeng Qu, Xi Wu, Zhen Dong, Xiang Gao,
Moli Huang, Jie Yan, Ling Yang , Yi Liu, Louis Ptacek & Ying Xu (2013) Dual roles
of FBXL3 in the mammalian circadian feedback loops are important for period
determination and robustness of the clock. Proceedings of the National
Academy of Sciences，110 (12) 4750-4755.
16. Niu YY, Shen B, Cui YQ, Chen YC, Wang JY, Wang L, Kang y, Zhao XY, Si W, Li W,
Xiang AP, Zhou JK, Guo XJ, Bi Y, Si CY, Hu B, Dong GY, Wang H, Zhou ZM, Li TQ, Tan
T, Pu XQ, Wang F, Ji SH, Zhou Q, Huang XX, Ji WZ, Sha JH. (2014) Generation ofGene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in
One-Cell Embryos. Cell 156: 836-843

17. Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins A, Lyer V,
Huang XX, Skarnes, WC (2014) Efficient genome modification by CRISPR-Cas9
nickase with minimal of-target effects. Nature methods 11: 399-402

53

54

RIKEN BioResource Center (BRC), Tsukuba
Institute Director/ President

Yuichi Obata

AMMRA contact person

Yuichi Obata

Phone+81-29-836-9144

E-mailyobata@rtc.riken.go.jp

Institute address

3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan

Websitehttp://www.brc.riken.jp/inf/en/index.shtml

55

RIKEN BioResource Center (BRC), TsukubaPart I – Institution Briefing

RIKEN BioResource Center (BRC) was established in 2001 at Tsukuba, Japan.

Type and Funding source: Not-for-profit and government-funded institution.
Experimental Animal Division (http://www.brc.riken.jp/lab/animal/en/ ) has
been designated as the core facility for mouse resources by the National
Bioresource Project (NBRP, http://www.nbrp.jp/index.jsp ) of the MEXT, Japan
since FY2002.
A searchable database: http://www2.brc.riken.jp/lab/animal/search.php
A contact address: animal@brc.riken.jp
Cryopreservation and advanced reproductive technology: Bioresource
Engineering Division headed by Dr. Atsuo Ogura.
http://www.brc.riken.jp/lab/kougaku/indexE.html
A phenotyping platform: Japan Mouse Clinic lead by Dr. Shigeharu Wakana.








http://www.brc.riken.jp/lab/jmc/mouse_clinic/en/index.html
RIKEN BRC is a founding member of Asian Mouse Mutagenesis & Resource
Association (AMMRA, http://www.ammra.info/), The Federation ofInternational Mouse Resources (FIMRe, http://www.fimre.org/ ), Asian Mouse
Phenotyping Consortium (AMPC, http://ampc.asia ), and Asian Network of
Research Resource Centers (ANRRC, http://www.anrrc.org/ ).
56







RIKEN BRC registers its mouse strains in the International Mouse Strain Resource(IMSR, http://www.findmice.org/ ), a one-stop database of FIMRe, andgene-trap ES cells in the International Gene Trap Consortium (IGTC,
http://www.genetrap.org/index.html).
Japan Mouse Clinic and Experimental Animal Division have been participating in
the International Mouse Phenotyping Consortium (IMPC) since September,2011. http://www.mousephenotype.org/
RIKEN BRC has been offering training courses on advanced technologies for theuse of mouse resources to Asian scientists and technicians

The Australian Phenomics Facility has joined as a new member of the AMMRA on May 18, 2013
in the 2013 AMMRA/AMPC meeting held at RIKEN BRC, Tsukuba. Directors tightly gathered forthe future.

57

Part II - Mouse/Rat Strain Resource

RIKEN BRC Experimental Animal Division
Repository Director

Atsushi Yoshiki, Ph.D., Head

Emailyoshiki@brc.riken.jp

Repository personnel

PI: 1
Senior Research Scientist: 2
Senior Technical Scientist: 2
Research Scientist: 1
Technical Staff: 10
Assistant:2
Contract Staff: 52

WebsiteMouse strain Resource

http://www.brc.riken.jp/lab/animal/en/
Live: 599
Cryopreserved: 5158
ES/iPS cells a:1847
BAC clones b: C57BL/6N, MSM

Rat strain Resource

Cryopreserved: 9160
ES/iPS cells a: 1
BAC clones b: F344, LE, ACIIMSR registered?

● Yes
○ No

Source of resource● Government funding generated● Deposited by researchers

Notes:
a: 1,748 gene-trap ES cell clones developed by Dr. Ishida, 92 mouse ES cells, 7 mouse
iPS cells and 1 rat iPS cell are distributed from RIKEN BRC Cell Engineering Division(Cell Bank) (Dr. Yukio Nakamura, cellqa@brc.riken.jp).
b: Distributed from RIKEN BRC Gene Engineering Division (DNA Bank) (Dr. Yuichi
Obata, dnabank@brc.riken.jp).
c: back-up storage of NBRP-rat including embryos and ENU-mutant sperm archive.

58

Consomic, 41,
1%
Recombinant
inbred, 62, 1%

Wild-derived,
595, 10%Inbred andspontaneous
mutant, 232,
4%

Transgenic,
1,921, 34%

ENU-induced
gene-driven
mutant, 593,
10%
ENU-induced
phenotypedriven mutant,
10, 0%

MutantMouse Strains

Gene-trap, 303,
5%

Targeted
knockout,
2,000, 35%59

Dual color conditional reporter: Hasegawa Y,
Daitoku Y, Sekiguchi K, Tanimoto Y, Mizuno-Iijima
S, Mizuno S, Kajiwara N, Ema M, Miwa Y, Mekada
K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K.
Novel ROSA26 Cre- reporter knock-in C57BL/6N
mice exhibiting green emission before and red
emission after Cre-mediated recombination. Exp
Anim 62: 295-304, 2013.
This figure reproduced from the above paper by the courtesy of
the authors and the publisher.

Ins1-cre: Hasegawa Y, Daitoku Y, Mizuno S,
Tanimoto Y, Mizuno-Iijima S, Matsuo M, Kajiwara
N, Ema M, Oishi H, Miwa Y, Mekada K, Yoshiki A,
Takahashi S, Sugiyama F, Yagami K. Generationand characterization of Ins1- cre-driver C57BL/6N
for exclusive pancreatic beta cell-specific Cre-loxP
recombination. Exp Anim 63, 183-191, 2014.
Figure: LacZ staining of Ins1-cre x ROSA-lacZ reporeter

Dentate gyrus granular neuron specific Cre,Dock10-cre: Discovery of a novel neural circuitfrom DG to CA2 Kohara K, Pignatelli M, Rivest
AJ, Jung HY, Kitamura T, Suh J, Frank D, Kajikawa K,
Mise N, Obata Y, Wickersham IR, Tonegawa S. Cell
type-specific genetic and optogenetic tools reveal
hippocampal CA2 circuits. Nat Neurosci 17: 269-279, 2014.
Figure: LacZ staining of Dock10-cre x ROSA-lacZ reporeter

Entorhinal cortex layer II island cells specific Cre,
Wfs1-cre: Discovery of a novel neural circuit for
temporal association memory. Kitamura T,
Pignatelli M, Suh J, Kohara K, Yoshiki A, Abe K,
Tonegawa S. Island Cells Control Temporal
Association Memory. Science 343(6173): 896-901,
2014.
Figure: LacZ staining of Wfs1-cre x ROSA-lacZ reporeter

Dual color reporter and tissue-specific cre mice for conditional experiments
60

Part III - Special Announcement
The 60th annual meeting of Japanese Association of Laboratory Animal Science(JALAS) was held on May 15-17, 2013 at Tsukuba International Congress Center by Dr.
Yuichi Obata, Director of RIKEN BRC.
2013 AMMRA & AMPC business meeting was held on May 17-18th, 2013 at RIKEN BRC.
The Australian Phenomics Facility has joined as a new member of the AMMRA.

The 27th Molossinus Colloquium joint with RIKEN Symposium entitled “The genome
design technology and disease models” was held on June 28-29 in Tsukuba.
The 2nd NANJING UNIVERSITY MARC/RIKEN BRC International Summer Intensive
Course of the Mouse was held on July 29-31, 2013 at MARC of Nanjing University.
Keynote speakers: Dr. Tom Weaver, Director of MRC Mary Lyon Centre (UK) and Prof.
David Wasserman, Director of Mouse Metabolic Phenotyping Center, Vanderbilt
University
The 3rd NANJING UNIVERSITY MARC/RIKEN BRC International Summer Intensive
Course of the Mouse was held with topics of Brain Science on July 28-30, 2014
(below photo). The Course was supported partially by the co-sponsorship of Japan
Society for the Promotion of Science (JSPS) and Natural Science Foundation of China(NSFC) within the framework of Bilateral Joint Research Projects/Seminars.
The 4th NANJING UNIVERSITY MARC/RIKEN BRC International Short Summer Courseof the Mouse is planned to take place from July 27 (Mon) to 29 (Wed), 2015 at MARC,
Nanjing University. We will focus on the heart development and diseases.

The 3rd NANJING UNIVERSITY MARC/RIKEN BRC International Summer Intensive
Course of the Mouse held on July 28-30, 2014 at RIKEN BRC, Tsukuba. Eighteen
trainees from 5 countries participated in the course.

61

62

In Memoriam, Dr. Kazuo Moriwaki“It is our great sorrow to announce death of Dr. Kazuo Moriwaki.”-reproduced from RIKEN BRC web site on November 23, 2013

Dr. Moriwaki died of colon cancer in the early morning of November 23, 2013. He was 83
years old. Until his death, he was Special Consultant to RIKEN BioResource Center (BRC).

In his career, he served as Director of RIKEN BRC, Director of RIKEN Tsukuba Institute andVice Director and Honorary Professor of National Institute of Genetics.
Dr. Moriwaki was a member of a committee for establishment of a bioresource center in
RIKEN and appointed as the Founding Director of RIKEN BRC in 2001.

He, as the first director of a specialized center focusing biological resources ever establishedin Japan, has built successfully a solid foundation for the current activities in the BRC bylaying out institutional structure and gaining support from government and scientificcommunity.

Dr. Moriwaki was a renowned world leader in Mouse Genetics and trained numerous
scientists in his career.

Since he was diagnosed as colon cancer two years ago, he had been fighting against thecancer. Sadly, he succumbed to it. He is survived by his wife, Sachiko, two daughters and four
grandchildren.

All of us at the Center mourn Dr. Moriwaki's death. At the same time, we are reconfirming
our determination that we will do our best to realize Dr. Moriwaki's dreams and succeed his
spirit.
Sincerely yours,
RIKEN BioResource Center
References
Yuichi Obata. Greeting in RIKEN BRC Annual Report 2013-2014. p.5, 2014.
URL http://en.brc.riken.jp/info/pdf/14annual/05.pdf
Toshihiko Shiroishi. “Legacy of Dr. Kazuo Moriwaki” (1930-2013). Mammalian Genome 25: 193-194,
2014.

63

Dr. Kazuo Moriwaki and all AMMRA/AMPC participants on May 18, 2013 at RIKEN BRC.

“His contribution has benefited numerous researchers and scientists.”

The passing of Dr Moriwaki saddens us. His contribution in the field of laboratory

animals’ science has benefited numerous researchers and scientists in understanding
the characters of the different mouse strains better. His absence has left an
immeasurable void in the field that will be a challenge to his successor. We are
grateful to Dr Moriwaki for his contributions.
-Biological Resource Centre(BRC), A*STAR, Singapore“His contributions are well-known & wide-ranging.”

The contributions of Dr. Moriwaki to the field of life science, in particular to genetics
in wild mice, are well-known and wide-ranging. We are much the worse for being
deprived of his advice and vast experience. We would like to express our regret andsorrow over Dr. Moriwaki's passing.
-Center for Animal Resources and Development (CARD), Kumamoto

64

“He was a pioneer and leader in laboratory animal science not only in
Asia but also in the world.”

I remember Dr. Moriwaki who was always smiling and readyto give an advice for being better Resource Center. I met him
at the first time in the FIMRe meeting held in RIKEN BRC in2006. In the last year, I met him and took the photo as below.
He was a pioneer and leader in laboratory animal science
not only in Asia but also in the world. Although he has been
passed away last November, I and my colleagues at KRIBB
will remember him. I am praying he sleep the eternal sleep
with peace.
May his soul rest in peace!
Dr. Hyoung-Chin Kim-Head of Laboratory Aniamal Resource Center, KRIBB“Wish he is happy and at peace where he is.”IDM faculty members offer our deepest condolences on the death of Dr. Moriwaki.
Wish he is happy and at peace where he is.
-Developmental Biology and Molecular Medicine, Shanghai“To us, Dr. Moriwaki is a role model, an icon, and a legend.”

He is a gentle giant in the science of mouse genetics. We sure will miss him forever.
-National Laboratory Animal Center, NARLabs, Taiwan

65

66

The Curriculum Vitae of Dr. Kazuo Moriwaki
Birth date and place
1930
November 4, Tokyo
Education
1954
Bachelor of Zoological Science, University of Tokyo
1959
Doctor of Science, University of Tokyo
Employment
1959
Research scientist, National Institute of Genetics(NIG), Mishima, Japan
1964
Postdoc Fellow, Mammalian Genetics Center,
University of Michigan, USA
1967
Laboratory Head, Cytogenetics Department, NIG
1984
Professor, Cell Genetics Department, NIG
1992
Vice Director, NIG
1994
Professor Emeritus, NIG
1994
Professor, School of Engineering, Fukuyama University, Fukuyama
1995
Vice President of the Graduate University for Advanced Studies, Hayama
2001
Director, RIKEN BioResource Center
2003
Director, RIKEN Tsukuba Institute and BioResource Center
2005
Special Consultant to RIKEN
2007
Special Adviser to RIKEN BioResource Center
Public office membership
1994
The Science Council of Japan
Board membership
1986
Board member, Heiwa Nakajima Foundation
1991
President, Genetics Society of Japan
1994
President, Japanese Association of Laboratory Animal Sciences
1996
President, Association for Propagation of the Knowledge of Genetics
1998
Board member, Yamada Science Foundation
Awards1982
The Zoological Society of Japan Scientific Award
2004
The Japanese Association of Laboratory Animal Science Achievement Award
2008
The Mammal Society of Japan Scientific Award
2013
The Prize for Science and Technology (Research Category) by the MEXT,
Japan

67

68

AMMRA Annual Meetings

1st AMMRA meeting, Shanghai, China, Nov., 22-24, 2006
2nd AMMRA meeting, Nanjing, China, Nov., 14-16, 2007
3rd AMMRA meeting, Daejeon, Korea, Oct., 23-24, 2008
4th AMMRA meeting, Kumamoto, Japan, Dec. 17-18, 2009
5th AMMRA pre-meeting, Hong Kong, Aug., 26-27, 2010
5th AMMRA meeting, Taipei, Taiwan, Nov., 8-11, 2010
6th AMMRA meeting, Biopolis, Singapore, Nov., 29-Dec., 2, 2011
7th AMMRA meeting, Nanjing, China, Mar., 15-17, 2012
8th AMMRA meeting, Tsukuba, Japan, May, 18, 2013
9th AMMRA-AMPC business meeting, Canberra, Australia, Oct., 21, 2014

69

70

CONTACT LIST

Contact List
(Accumulative list of attendants from past AMMRA meetings)
ARAKI, Kimi
IRDA, Kumamoto University, Japanarakimi@gpo.kumamoto-u.ac.jp
ANDREWS, Dan
Australian Phenomics Facility, JCSMR, ANU
dan.andrews@anu.edu.au
ARAKI , Masatake
Kumamoto University, Japan
maraki@gpo.kumamoto-u.ac.jp
ARAKI, Kimi
Kumamoto University, Japanarakimi@gpo.kumamoto-u.ac.jp
BERTRAM, Edward
Australian Phenomics Facility, JCSMR, ANU
edward.bertram@anu.edu.au
BOK, Jin-woong
Yonsei University, Korea
bokj@yuhs.ac
CHEN, Chih-Cheng
Taiwan Mouse Clinic, Institute of Biomedical Sciences, Academic Sinica, Taiwan
chih@ibms.sinica.edu.tw
CHOI, Jae-Hoon
Hanyang University, Korea
jchoi75@hanyang.ac.kr
CHIN, Hsian-Jean
National Laboratory Animal Center
National Applied Research Laboratories, Taiwan
geneichin@nlac.narl.org.tw
CHUNG, Bon-Chu
Institute of Molecular Biology, Academia Sinica, Taiwan
mbchung@sinica.edu.tw
71

DENG, Hongkui
Department of Cell Biology
The college of Life Sciences, Peking University, China
hongkui_deng@pku.edu.cn
ENDERS, Anselms
The John Curtin School of Medical Research, ANU
anselm.enders@anu.edu.au
FEI, Jian
Shanghai Research Center for Biomodel Organism, China
Jfei@sibs.ac.cn
FIELD, Matt
Australian Phenomics Facility, JCSMR, ANU
matt.field@anu.edu.au
FOWLER, Suzie
Australian Phenomics Facility, JCSMR, ANU
suzanne.fowler@anu.edu.au
GAN, Ke
Model Animal Research Center, Nanjing University, China
ganke1131105@163.com

CONTACT LIST

University of Manitoba; Canada
hicksgg@cc.umanitoba.ca
IKAWA, Masahito
Research Institute for Microbial Diseases, Osaka University, Japanikawa@biken.osaka-u.ac.jp

72

CONTACT LIST

GAO, Xiang
Nanjing University; China
gaoxiang@nju.edu.cn
GONDO, Yoichi
RIKEN BioResource Center, Japan
gondo@brc.riken.jp
HUANG, Ya-Fang
National Laboratory Animal Center, Taiwan
yafanghuang@nlac.narl.org.tw
HICKS, Geoffrey

IWAKURA, Yoichiro
Center for Experimental Medicine and Systems Biology
Institute of Medical Science, University of Tokyo, Japaniwakura@ims.u-tokyo.ac.jp
Jung, Han-Sung
Yonsei University College of Dentistry, Korea
hsjung@yuhs.ac
JIANG, Si-Tse
National Laboratory Animal Center
National Applied Research Laboratories, Taiwan
stjiang@nlac.narl.org.tw
KAKUTA, Shigeru
Center for Experimental Medicine and Systems Biology
Institute of Medical Science, University of Tokyo, Japan
kakuta@ims.u-tokyo.ac.jp
KANEDA, Hideki
RIKEN BioResource Center, Japanhkaneda@brc.riken.jp
KANG, Hoil
National Institute of Food and Drug Safety Evaluation
Korea Food and Drug Administration, Korea
kanghoil@korea.kr
KATOH, Hideki
Institute for Experimental Animals
Hama-matsu University School of Medicine, Japanhideki-k@hama-med.ac.jp
KIM, Hyoung-Chin
Korea Research Institute of Bioscience and Biotechnology, Korea
hckim@kribb.re.kr
KOROGI, Rie
Institute of Resource Development and Analysis, Kumamoto University, Japankorogi@kumamoto-u.ac.jp
KANG, Byeong-Cheol
Seoul National University, Korea
bckang@snu.ac.kr
73

KOFLE, Jennifer
Australian Phenomics Facility, JCSMR, ANU
jennifer.kofler@anu.edu.au
LEE, Han-Woong
Yonsei University (Department of Biochemistry), Korea
hwl@yonsei.ac.kr
LEE, Ho
National Cancer Center, Korea
ho25lee@ncc.re.kr
LEE, Jong-Min
Yonsei University, Korea
min@yuhs.ac
LI, Zheng-hua
Institute of Resource Development and Analysis, Kumamoto University, Japanliseika@kumamoto-u.ac.jp
LI, Wan-bo
Disease Resource Experimental Animal Center,
Institute of laboratory Animal Science,
Peking Union Medical College and Chinese Academy of Medical Sciences,

CONTACT LIST

LIANG, San-Chi
National Laboratory Animal Center,
National Applied Research Laboratories, Taiwan
simon@nlac.narl.org.tw

74

CONTACT LIST

China
Li_Wanbo2006@yahoo.com.cn
LIN, Shu-Wha
College of Medicine, National Taiwan University,
Department of Clinical Laboratory Sciences and Medical Biotechnology, Taiwan
mtshuwha@ntu.edu.tw
LIN, Chia-Yu
National Laboratory Animal Center,
National Applied Research Laboratories, Taiwan
vetchiayu@nlac.narl.org.tw

MANICKAM, Arun Kumar
Biological Resource Centre, Singapore
arunkumar@brc.a-star.edu.sg
MASUYA, Hiroshi
RIKEN BioResource Center, Japanhmasuya@brc.riken.jp
MOORE, Mark W.
International Mouse Phenotyping Consortium, USA
mwmoore880@comcast.net
MORRIS, Lucy
Australian Phenomics Facility, JCSMR, ANU
lucy.morris@anu.edu.au
MURATA, Takuya
RIKEN BioResource Center, Japan
takuyam@brc.riken.jp
MUKUNOKI, Ayumi
Center for Animal Resources and Development (CARD), Kumamoto University,Japan
117p2032@st.kumamoto-u.ac.jp
NAKAGATA, Naomi
Kumamoto University, Center for Animal Resources and Development, Japannakagata@kumamoto-u.ac.jp
NAM, Ki-Hoan
Korea Research Institute of Bioscience and Biotechnology, Korea
namk@kribb.re.kr
OBATA, Yuichi
RIKEN BioResource Center, Japanyobata@rtc.riken.go.jp
OKABE, Masaru
Osaka University; Japanokabe@gen-info.osaka-u.ac.jp
OH, Goo Taeg
Ewha Womans University, Laboratory of Cardiovascular Genomics
Division of Life and Pharmaceutical Sciences, Ewha Womans University, Korea
gootaeg@ewha.ac.kr
75

PARK, Jong Hoon
Sookmyung Women’s University, Korea
parkjh@sookmyung.ac.kr
PONNIAH, Sathivel
Biological Resource Center, Singapore
ponniah_sathivel@brc.a-star.edu.sg
QIN, Chuan
The Chinese Association for Laboratory Animal Science (CALAS)
The institute of Laboratory Animal Science, CAMS&PUMC, China
calas_ie@126.com
Qi, XinModel Animal Research Center, Nanjing University, China
qixin@nicemice.cn
READ, Stuart
Australian Phenomics Facility, JCSMR, ANU
stuart.read@anu.edu.au
SARUKI, Shigefumi
RIKEN Tsukuba Planning Section, Japanssaruki@riken.jp

CONTACT LISTSEONG, Je Kyung
Seoul National University; Korea
snumouse@snu.ac.kr
SERIKAWA, Tadao
Kyoto University; Japan
serikawa@anim.med.kyoto-u.ac.jp
SUZUKI, Tomohiro
Riken BioResource Center, Japansuzukito@brc.riken.jp
TAKABAYASHI, Shuji

CONTACT LIST

Institute for Experimental Animals,
Hamamatsu University School of Medicine, Japan
Shuji@hama-med.ac.jp76

TAKEDA, Shuko
The Japanese Association for Laboratory Animal Science (JALAS);
Osaka University Graduate School of Medicine; Japan;
takeda@cgt.med.osaka-u.ac.jp
TAKEO, Toru
Center for Animal Resources and Development (CARD), Kumamoto University,Japantakeo@kumamoto-u.ac.jp
TERAOKA, Nobuaki
Beijing Office, Riken, China
teraoka@riken.org.cn
VITLER, Laura
The John Curtin School of Medical Research, ANU
laura.vitler@anu.edu.au
WAKANA, Shigeharu
RIKEN BioResource Center; Japanswakana@brc.riken.jp
WANG, Chi-Kuang Leo
National Laboratory Animal Center,
National Applied Research Laboratories, Taiwan
cklwang@narlabs.org.tw
WANG, Zhu-Gang
Shanghai Research Center for Model Organisms; China
zhugangw@shsmu.edu.cn
WEAVER, Thomas A.
UK Medical Research Council Harwell; UK
t.weaver@har.mrc.ac.uk
WHITTLE, Belinda
Australian Phenomics Facility, JCSMR, ANU
belinda.whittle@anu.edu.au
WINSLADE, Steve
Australian Phenomics Facility, JCSMR, ANU
steve.winslade@anu.edu.au77

WU, XiaohuiFudan University; China
xiaohui_wu@fudan.edu.cn
WU, Phil
Australian Phenomics Facility, JCSMR, ANU
philip.wu@anu.edu.au
XU, Jiake
University of Western Australia
jiake.xu@uwa.edu.au
WURST, Wolfgang
Helmholtz Zentrum München; Germany
wurst@helmholtz-muenchen.de
XIE, Gui-Lin
Chian Biotech, Labzhou Institute of Biological Product, China
guilxie@public.lz.gs.cn
XU, Ping
Shanghai Laboratory Animal Center, Chi-nese Academy of Sciences, &Shanghai SLAC Laboratory Animal Co., Ltd., China
xuping@saccas.com

yamamura@gpo.kumamoto-u.ac.jp
YAMAZAKI, Yukiko
Genetic Informatics Laboratory, Center for Genetic Resource Information,
National Institute of Genetics, Japanyyamazak@lab.nig.ac.jp

78

CONTACT LIST

YANG, Zhi-Wei
Institute of Laboratory Animal Science
Chinese Academy of Medical Sciences, China
YAMADA, Gen
Department of Developmental Genetics Research
Wakayama Medical University, Japangensan7@wakayama-med.ac.jp
YAMAMURA, Ken-ichi
Center for Animal Resources and Development (CARD),
Kumamoto University, Japan

CONTACT LIST

YANG, Xiao
Genetic Laboratory of Development and Diseases,
Beijing Institute of Biotechnology, China
yangx@nic.bmi.ac.cn
YEN, Jeffrey J.Y.
Academia Sinica, Taiwan
bmjyen@ibms.sinica.edu.tw
YEOM, Su-Cheong
Seoul National University, Korea
scyeom@snu.ac.kr
YOSHIKI, Atsushi
RIKEN BioResource Center; Japan
yoshiki@brc.riken.jp
YU, Dae-Yeul
Korea Research Institute of Bioscience and Biotechnology, Korea
dyyu10@kribb.re.kr
ZHANG, Lianfeng
Institute of Laboratory Animal Science
Chinese Academy of Medical Sciences
Peking Union Medical College, Chinazhanglianfeng@cnilas.pumc.edu.cn
ZHAO, Jing
Model Animal Research Center, Nanjing University
zhaojing@nicemice.cn

79

2013‐2014 A
AMMRA Annual ReportR
Editing Gro
oup/
Chi‐Kuang Leo W
Wang / Hsiaan‐Jean Gen
nie ChinMeng‐Feeng Ryan Lin
n/Snow Yang

Publishi ng Date：M
March, 20155