BBARL, Inc.

Human Mesenchymal Stem Cells (hMSCs) Serum-Free Medium for cell proliferation

Xyltech™ Growth MSC
1. Product features
This product is a culture medium suitable for the growth of human mesenchymal stem cells (hMSCs).
When used in combination with Xyltech™ MSC-01 Xeno-Free or Xyltech™ MSC-02 Animal-Free, hMSCs
proliferation can be controlled as needed. The basal medium “Xyltech™ Growth MSC Medium” should be
used with the additive “Xyltech™ Growth MSC Supplement XF” or “Xyltech™ Growth MSC SupplementAF”. In addition, there is no need to pre-coat culture vessels.
< Product content >CAT. #VolumeNote(BBARL/NIPRO)
XyltechTM Growth MSC Medium
10411/87-331
500 mL
Basal medium (*Animal-Free)
TMXyltech Growth MSC Supplement XF
10412/87-332
10 mL**Xeno-Free supplement
TM
Xyltech Growth MSC Supplement AF
10413/87-333
10 mL*Animal-Free supplement*Animal-Free: It contains no ingredients derived from animals.
**Xeno-Free: It contains human-derived components and is free from any other animal-derived ingredients.
Product Name

2. Precautions for use
Xyltech™ Growth MSC does not contain substances that neutralize trypsin activity. When subculturing
cells with trypsin, it is strongly recommended that the trypsin activity be sufficiently neutralized with atrypsin inhibitor. Dilution washing alone does not completely remove trypsin activity and the remaining
protease activity will reduce subsequent cell growth.
This product is a research reagent. It cannot be used for human or animal treatment or diagnostic purposes.
3. Storage
Store Xyltech™ Growth MSC Medium in a cool, dark place (2-8°C). Store Xyltech™ Growth MSC
Supplement XF and Xyltech™ Growth MSC Supplement AF in a freezer (-20°C).
4. Thawing and preparation
Quickly thaw a frozen supplement in a 37°C water bath, being careful not to submerge the entire bottle.
Watch the supplement closely; when the whole contents melt, please take it out immediately from thewater bath. Once thawed, the supplement should be stored at 2-8°C and added to the basal medium within
2 weeks. After the supplement is added to the basal medium, use it immediately.
It is recommended to prepare the required volume of the medium just before use. For small volume
preparation, add 1/50 volume of Xyltech™ Growth MSC Supplement XF or Xyltech™ Growth MSC
Supplement AF to Xyltech™ Growth MSC Medium. Additionally, do not freeze the medium after adding
the supplement to avoid deterioration of some active ingredients.
Fine particles may be observed but there is no influence on quality.

5. Example of cell culture protocol for normal human adipose-derived stem cells (ADSCs) cultureusing Xyltech™ Growth MSC
5-1. Cells and reagents・Normal human ADSCs (100 mm-dish)・Xyltech™ Growth MSC Medium(BBARL CAT. #10411)
・Xyltech™ Growth MSC Supplement XF(BBARL CAT. #10412)
or Xyltech™ Growth MSC Supplement AF(BBARL CAT. #10413)
・r-TE (Recombinant Trypsin/EDTA Solution)(NIPRO CAT. #87-974)
・s-TI (Synthetic Trypsin Inhibitor Solution)(NIPRO CAT. #87-975)
・D-PBS (-)
5-2. Cell culture of normal human ADSCs
1. Before using Xyltech™ Growth MSC, add the entire amount of Xyltech™ Growth MSC Supplement
XF or Xyltech™ Growth MSC Supplement AF (thawed before use) to Xyltech™ Growth MSC
Medium and mix well.
2. Warm the Xyltech™ Growth MSC, D-PBS (-), r-TE, and s-TI in a 37°C water bath.
3. Remove the culture supernatant of selected normal human ADSCs that have reached approximately
80% confluence (sub confluence).
4. Rinse the cell layer with 5 mL of D-PBS (-).
5. Add 0.5 mL of r-TE and incubate at 37°C for approximately 2 minutes.
6. Add 0.5 mL of s-TI, mix well, gently pipette up and down several times, collect cells from the dish,and centrifuge at 1,000 rpm, for 5 minutes.
7. Aspirate the supernatant and add the appropriate amount of Xyltech™ Growth MSC to resuspend
the cells and seed into a new tissue culture dish.
8. The cells will become confluent within 2-4 days when cultured in Xyltech™ Growth MSC. Start
subculture and/or experiments with the cells.
9. For experiments requiring a reduced growth rate, the cells can be cultured for 3 days in Xyltech™
MSC-01 Xeno-Free or Xyltech™ MSC-02 Animal-Free growth suppressive medium (refer to protocol
for suppressive growth control using Xyltech™ MSC-01 Xeno-Free, BBARL CAT. #10401 orXyltech™ MSC-02 Animal-Free, BBARL CAT. #10402).
*The protocol is based on experimental results. It may be necessary to adjust seeding density, andpassage timing according to the cells. This protocol is intended for research purposes only.

6. For Inquiries about products
Bourbon Biomedical Advanced Research Laboratories, Inc. (BBARL, Inc.)
1-3-1, Ekimae, Kashiwazaki-city, Niigata,
TEL: +81-257-23-2769 E-mail: support@bourbon-barl.co.jp
URL: https://www.bourbon-barl.co.jp/eg/

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