Biospecimens Product Catalog

The Patient, their Hope,and our Commitment
Hope enables the patient to endure the adversity and pain of disease. Hope motivatespeople to participate in clinical research studies and biospecimen donation. Time is theworst enemy of hope. As time passes, it prolongs and intensifies pain while diminishing
the body and spirit.
Our mission is to restore hope by speeding the development of innovative diagnostics
and effective treatments to improve care. We believe that the passing of time should
increase the joy of the patient and their loved ones rather than extinguish it.
At Discovery Life Sciences, we commit to acquiring the leading technologies
and employing the most dedicated and brightest minds from every culture, walk oflife, and region of the world. We come together to accelerate and advance science. We
accomplish this by partnering with researchers in industry, academia, and governments—enhancing their abilities to complete their vital work faster by providing them withthe highest quality products and services. We commit to acting ethically and withintegrity while strictly adhering to all laws and regulations.
We will never waiver from these commitments…

Because Patients Are Waiting®.

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TABLE OF CONTENTS
Science at Your Service™

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Dissociated Tissue & Tumor Cells (DTCs)

8

Formalin-Fixed Paraffin-Embedded (FFPE) Tissues

12Bone Marrow Mononuclear Cells (BMMCs)

14

Peripheral Blood Mononuclear Cells (PBMCs)

16

Matched Sample Sets

18

SpecimenSeqTM Biospecimens

20

Blood Products

22

Non-Blood Biofluids

24

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Science at your Service™
Discovery combines the world’s largest
commercial biospecimen inventory andprocurement network with preeminent
multi-omic service laboratories.
Our consultative, innovative approachhelps our customers and partners overcome
obstacles to reach quality results faster.

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Biospecimens and Multi-Omic Services – Under One Roof​

Assay-Ready
Biospecimens
The World’s Largest Biospecimen Inventory
Powered by Discovery Partners® Networkand Supported by Multi-Omic Services

Discovery
Genomics
DTCs, FFPE, BMMCs,
PBMCs, Matched Sets,SpecimenSeqTM, BloodProducts,
Non-Blood Biofluids

WES, RNA-Seq,
Single-Cell,
Targeted-Seq,
Pan-Cancer, WGS,
HiFi LRS,
Epigenomics, qPCR

Proteome Center
Targeted & Untargeted
LC-MS/MS, Luminex,
Olink (Explore HT,Explore, Target, Focus,Flex), Multi-omics

Gentest®
MolecularPathology

In Vitro Preclinical
IND-Enabling ADMETand Hematopoietic Drug
Toxicity and Efficacy
Solutions

AllCells®
Cell & Gene Therapy
Leukopaks (Mobilized/GMP/RUO), Isolated
Cells, 10k+ recallable/
HLA-Typed Donors

Flow Cytometry& Cell Biology

IHC, mIF, ISH,Histology,
Digital Pathology,
Quantitative
Gene Expression

Clinical Flow Cytometry,
FACS, Dissociations,Single Cell & Spatial
Library Prep,
In Vitro Culture.

5Assay-Ready
Biospecimens
The Best Selection for the Best Data
Optimize your projects with unmatchedquality, characterization, selection, and scale

6WHO L E BLOOD

S WA B S

L EUKOPA K S

NO RMAL
ADJACE NT
TISSUEDTC S

PLASMA/SE RUM

TUMO R TISSUEFFPED O U B L E -SPUN
PL ASMAP ER I P H ER A L B LO O D

HE PATO CYTE S(GE NTE ST ®)FL ASH FROZ E N

BU F F Y C OATBO NE MARRO W

BMMCSP BMC S

N ON -B LOOD
B I OF L UI DS

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Dissociated Tumor &Tissue Cells (DTCs)
An ideal alternative to fresh tissues without theassociated logistical challenges• Representation of the full tumor microenvironment• High pre-freeze (83%) and post-thaw (69.7%) viability• Dissociated tumor cells from 50+ cancer indications
and characterized for MSI, HPV,
PD-L1, high-resolution HLA, and ER/PR/HER2• Normal dissociated tissue cell inventory includes lung,colon, ovary, tonsil, and liver• Available in matched sets (18) and SpecimenSeqTM (20)

8DTCs

Fresh solid tumor resections are placed in a proprietary tissue storage solution
and shipped in a NanoCool™ Cooling System to Discovery’s Custom Biospecimen
Processing Laboratory.
Upon receipt, tumors undergo a proprietary mechanical and enzymatic digestion
to the single cell level. This digestion takes approximately 1 hour. Following
digestion, cells are washed and cryopreserved.
A QC aliquot is thawed to provide a post-thaw cell count and viability, along withthe flow cytometry characterization every biospecimen receives.

1,400+PRODUCT VOLUMESTO RAGE CO N TA IN E R

STO R AG E ME D IA

1.0 mL

1.0 mL MatrixTM cryovial

Cryopreserved in CryoStor® CS10Unique Patients

PROCESS TIMES TO RAGE T EM P E R ATU R E & C O N D ITIO N S

PAC K AG E C O N D I T I O N I N G

Received within 24-72
hours and immediately
processed

Liquid nitrogen vapor phase (LN2)-80°C (dry ice), LN2 dewars
available upon request

DTCs

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Flow Cytometry Report - Dissociated Tumor Cells
Flow Cytometry Report - Dissociated Tumor Cells
SKU: BTC1000-8A200009436031020MS

BTC1000-8A200009436031020MS
SKU: Tumor& Immune Cells
Tumor & Immune Cells

Indication: Lung Cancer, Adenocarcinoma

Indication: Lung Cancer,
Adenocarcinoma
HLA-A02HLA-A02

HLA-A02
HLA-A02

FMO

Every DTC comes with cell population
analysis & HLA-A02 status by flow
cytometry as well as base patient data
and redacted pathology report.
Colorectal Cancer

FMO

Gated onCD45+
GatedonCells
CD45+ Cells
CD4+ T Cells

CD4+ T Cells

CD8+ T Cells

B Cells

CD8+ T Cells

B Cells

Lung Cancer
NK Cells(Gated on CD3- Cells)

NK Cells(Gated on CD3- Cells)

Myeloid Cells

Monocytic/PMN Cells

Myeloid Cells© 2018 Discovery Life Sciences

www.dls.com© 2018 Discovery Life Sciences

10DTCsMonocytic/PMN CellsDo Not Distributewww.dls.com

Do Not DistributeDiscovery’s Proprietary Cell Dissociation
and Cryopreservation Process

Refer to DTCs User Guide for more detail
DTCs11

Formalin-Fixed
Paraffin-Embedded(FFPE) Tissue
The largest, most extensive characterized oncology
FFPE inventory• Digital H&E images are available for every FFPE block• Hyper-annotated® FFPE blocks, curls, and slides• Fortified by rigorous, 12-point quality assurance andtriple pathologist-review• Rapid sample delivery, usually within 10 business days
• Less than 2% return rate of FFPE blocks (industryaverage >10%)• Available in matched sets (18) and SpecimenSeqTM (20)12FFPEFFPE tissues are collected during surgery, used to diagnose patients,
and preserved for downstream analysis.
Oncology, non-oncology, and normal FFPE samples are available.
Oncology FFPE characterization includes SpecimenSeqTM, HPV, MSI,
and PD-L1.
FORMATS60,000+

STORAGE CONDITION

BlocksSlide Sets (4 or 5 µm)
Curls (10 µm)

Hyper-Annotated® FFPE Blocks
Room temperature

Custom-built TMAs
PACKAGE CONDITIONING
Ambient or refrigerated* uponrequest*Refrigeration may help reduce thechance of melting

Digital H&E images included for every FFPE block

LE A D TIME
Blocks and pathology data typically sent within 10 businessdays upon order placement.

FROM

18,000+Unique Patients

Domestic orders are delivered next day.
International orders usually deliver in 3-5 business days
unless specialty couriers are used.

FFPE

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Bone Marrow
Mononuclear Cells(BMMCs)Optimize cohort selection with immunophenotypicallycharacterized BMMCs• Disease-specific multiparametric flow characterization
with HLA-A02 status• Post-thaw cell counts and viability listed for optimal
sample selection• Contain normal and malignant cells, replicating
disease specific tumor microenvironment• Come with patient demographics information andredacted pathology report• Available in matched sets (18) and SpecimenSeqTM (20)

14BMMCsDiseased BMMCs are composed of both normal and malignant cells.
Immunophenotypes of malignant cells are readily identified by flow cytometry.
Normal BMMCs are collected via elective bone marrow aspirates from fully
consented healthy donors.

PRODUCT VOLUME

STORAGE CONTAINER

Minimum of 1M cells per aliquot
Cell counts up to 10M cells peraliquot are available

1.0 mL Matrix

TMcryovial

STORAGE MEDIA

Cryopreserved in 90% heat-inactivated
FBS +10% DMSO and undergo slow
freezing overnight at -80°C.

PROCESS TIME

STORAGE TEMPERATURE& CONDITIONSPACKAGE CONDITIONING

Received within 24 hours
and immediately processed

Liquid nitrogen vapor phase (LN2)-80°C (dry ice), LN2 dewars
available upon request

200+Unique Patients
Including Acute Myeloid
Leukemia (AML) andMultiple Myeloma (MM)Patients

BMMCs15

Peripheral Blood
Mononuclear Cells(PBMCs)
Accelerate your programs with extensive inventory anda prospective network of PBMCs• Diseased blood is collected just prior to the patient’s
next cycle of therapy• Cell counts typically range from 5M to 10M cells per mL• Available in matched sets (18) and SpecimenSeqTM (20)

16PBMCsDiseased blood is collected right before the patient’s next cycle of therapy
at standard laboratory appointments as dictated by the patient’s oncologist.
After thawing, cell counts and viability are determined using AOPI staining ona Nexcelom Cellometer which offers more accurate cell counts and viabilities.

PRODUCT VOLUME

1.0 mL~5M cells per aliquot

STORAGE CONTAINERSTORAGE MEDIA

1.0 mL MatrixTM cryovial

Cryopreserved in 90% heat-inactivated
FBS +10% DMSO and undergo slow
freezing overnight at -80°C.

STORAGE TEMPERATURE & CONDITIONSPACKAGE CONDITIONING

Vapor phase of liquid nitrogen (LN2)-80°C (dry ice), LN2 dewars
available upon request-150°C or colder

1,100+
Unique Patientsfrom 18 oncology indications

PBMCs17MatchedSample Sets
Fast-track in vitro drug screening and biomarker analysis• Prospective collections of high-quality patient andtime-matched sample sets
• Conduct fast and efficient comparative analyses
across various biospecimens• All matched sets include SpecimenSeqTM
NGS-characterized sample options

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Matched Sample Sets

FFPE Sets
Ideal for liquid biopsy discovery (tumor DNA + ctDNA + germline DNA), or use
across multiple assays requiring different inputs• FFPE + Double-Spun Plasma (Streck or K2EDTA) + Buffy Coat (optional)• FFPE + Double-Spun Plasma (Streck or K2EDTA) + Urine (Streck) + Buffy Coat (optional)• FFPE + DTCs
• FFPE + Flash Frozen Tissue• Custom FFPE+FFPE sets (biopsy + resection, primary + metastatic, NAT + tumor, etc)

DTC Sets

BMMC Sets

Ideal for studying immune response andtumor cell migration

Ideal for studying spatial and temporaldisease development• DTCs + PBMCs• BMMC + PBMC

• DTCs + FFPE

• Longitudinal BMMC + BMMC sets

• DTCs + PBMCs + FFPE
• DTC + Ascites Cells + Ascites SupernatantMatched Sample Sets

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SpecimenSeq™
BiospecimensConduct smarter R&D with clinically relevant, NGScharacterized samples• Verify the presence of biomarker targets in yourpopulations of interest• Confirm driver mutations and other clinically relevant
genomic variants such as SNVs, Indels, fusions, splicevariants, copy number variants, and TMB scores• Accelerate the timelines for identifying responders
versus non-responders and efficacy evaluations for
new cancer therapies

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SpecimenSeq™

SpecimenSeq™ FFPE

SpecimenSeq™ FFPE & Plasma

41,000+ variants from 500+ clinically relevant
genes across 19 oncology indications and 900+
unique patients

1,200+ variants from 50+ clinically relevant
genes across 30 oncology indications and 200+
unique patients

SpecimenSeq™ DTCs

SpecimenSeq™ BMMC & PBMC

800+ variants from 50+ clinically relevant
genes across 13 oncology indications and 200+
unique patients

33,000+ variants from 50+ clinically relevant
genes across 65 unique AML and MDS patients

Do you need custom characterization?
Our CAP-accredited, CLIA-certified, and GCLP-compliant service laboratories* offer comprehensive andscalable genomics, proteomics, molecular pathology, and flow cytometry services.

*Service laboratories are in various stages of compliance working toward full GCLP compliance, CAP accreditation and CLIA certification
SpecimenSeq™

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BloodProducts
Wide range of human diseased and normal
blood specimens• Highly specific patient targeting powered by Discovery
access to full electronic medical records (EMRs)• All blood specimens are ethically collected andIRB-consented• Donor information includes age, sex, ethnic
background, and clinical history• Blood collections are processed within hours or
shipped fresh overnight from the site of collection

22Blood ProductsBLOOD
BIOSPECIMENSPRODUCT VOLUME
AND CONTAINER

Plasma0.5 mL aliquots single-spun(Double-Spun andSingle-Spun)

1 mL aliquots double-spun
MatrixTM cryovialSerum

0.5 mL aliquots

Buffy Coat

0.3 mL in MatrixTM cryovial

Whole Blood

30-40 mL with various
container options available

STORAGE MEDIA

STORAGE TEMPERATURE &CONDITIONSPACKAGECONDITIONING-80°C

Shipped frozen at -80°C on dry ice-80°C

Shipped frozen at -80°C on dry ice-80°C

Shipped frozen at -80°C on dry ice

Custom collection, per project
specifications

Shipped fresh or frozen at -80°C
depending on project

Streck Cell-free DNA BCT®
orK2 EDTA in standard
blood collection tube

No anticoagulant used

K2 EDTA ingeneral blood collection tube

n/a*Specifically designed for preservation of circulating tumor cells (CTC) up to 7 days and cell-free DNA (cfDNA) up to 14 days at room temperature

Blood Products23

Non-Blood
Biofluids
Understand disease pathogenesis and identify novel
therapeutic targets• Large-scale, prospective collections of high-quality biofluids• Samples come with relevant patient data, health &clinical history

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Non-Blood Biofluids

B IOFLUID TYPEDESCRIPTIONPRODUCT VOLUME

Cerebrospinal Fluid (CSF)

Consented or remnant CSF samples available across a range
of demographic characteristics

1 mL diagnostic remnant, or up to 15 mL fromconsented collections

SwabRemnant or consented swab samples from multiple
respiratory viral tests and panels, including confirmed
negatives, influenza, and SARS-CoV-2

0.5 mL Universal Transport Medium®, or viraltransport medium from remnant collections, or up
to 3 mL from prospective collections

SputumSputum samples from multiple diagnostic tests

1 mL conical tubes, frozen

Aspirate

Culture positive endotracheal aspirates available across awide range of demographic characteristics.

1 mL conical tubes, frozen

Urine

Human urine across a range of disease states anddiagnostics tests

1 mL from diagnostic remnants, or up to 50 mLfrom consented collections.
FFPE + Plasma + Urine sets available

Non-Blood Biofluids

25Contact us at info@dls.com
or visit our website atwww.dls.com