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060-8589 ĄÎÍǁąŽǁfŇ
Introduction to “Realizing invisible plant-microbe interactions: current and future perspectives”
Atsushi J. Nagano1ƽMasanao Sato2
1

Faculty of Agriculture, Ryukoku University, Yokotani 1-5, Seta Ohe-cho, Otsu, Shiga 520-2194, Japan2

Department of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kitaku, Sapporo, Hokkaido 060-8589, Japan
Key words: plant-microbe interactions, next generation sequencing, imaging
DOI: 10.24480/bsj-review.8a1.00106

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Ļū#!¼¸'Ð" 6Bulgarelli, D., Rott, M., Schlaeppi, K., Ver Loren van Themaat, E., Ahmadinejad, N., Assenza, F., Rauf, P., Huettel, B., Reinhardt,
R., Schmelzer, E., Peplies, J., Gloeckner, F. O., Amann, R., Eickhorst, T., & Schulze-Lefert, P. (2012). Revealing structure andassembly cues for Arabidopsis root-inhabiting bacterial microbiota. Nature, 488: 91-95.
Hiruma, K., Gerlach, N., Sacristán, S., Nakano, R. T., Hacquard, S., Kracher, B., Neumann, U., Ramírez, D., Bucher, M,
O’Connell, R. & Schulze-Lefert, P. 2016. Root endophyte Colletotrichum tofieldiae confers plant fitness benefits that arephosphate status-dependent. Cell 165, 464-474
Lundberg, D.S., Lebeis, S.L., Herrera-Paredes, S., Yourstone, S., Gehring, J., Malfatti, S., Tremblay, J., Engelbrekston, A., Kunin,
V., Glavina del Rio, T., Edgar, R., Eickhorst, T., Ley, R.E., Hugenholtz, P., Tringe, S.G., & Dangl, J.L. 2012. Defining thecore Arabidopsis thaliana root microbiome. Nature 488, 86-90.
Márquez, L.M,, Redman, R.S., Rodriguez, R.J., & Roossinck, M.J. 2007. A virus in a fungus in a plant: three-way symbiosis
required for thermal tolerance. Science 315:513-515.
Stobble, A.H. & Roossinck, M.J. 2014. Plant virus metagenomics: what we know and why we need to know more. Front
Plant Sci. 5: 150.

A. J. Nagano & M. Sato -3
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3ĶŠğĦğĦɰɗþɌĦǤǮǩ;020-8550 ĶŠǡǜĵĻ±Ǎ 3-18-8
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;563-8577 ğɭŀƬǍĻȇµ 1-8-31
1

Ken-Taro Sekine 1, Hironobu Yanagisawa 2,3, Hidenori Nagai 4
Development of efficient diagnostic methods detecting plant viruses
Key words: deep sequencing, metagenomics, plant virus, virus diagnosis, virus hunting
1
University of the Ryukyus, Nishihara, Okinawa, 903-0213, Japan
2
Central Region Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Tsukuba,
Ibaraki, 305-8666, Japan
3
The United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate, 020-8550, Japan
4
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka,563-0026, Japan
DOI: 10.24480/bsj-review.8a1.00107


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RNA-Seq reveals hidden-interactions between wild plants and viruses
Mari Kamitani1, Atsushi J. Nagano2, Mie N. Honjo1 and Hiroshi Kudoh1
1Center for Ecological Research, Kyoto University,
Hirano 2-509-3, Otsu, 520-2113, Japan
2

Faculty of Agriculture, Ryukoku University, Yokotani 1-5, Otsu, 520-2194, Japan
Key words: plant viruses, RNA-Seq, virus ecology
DOI: 10.24480/bsj-review.8a1.00108

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yellows virus (BrYV)'ȴde novo assembly &×ú= 1 Ƭ+łǦEDuS Arabidopsis
partitivirus1 (AhPV1) +ǪȺƬ+EDuSŝ¶=ŭã+EDuSÑ+ƦƯ&,òȕ+EDuSǥ#<'<6ȴ|ǥĆ)7Ĩ:=<ȴȷƬ+þŜƄ?āǶ*7+&,×Ƴ+Ƭŀ­ǝƦƯ&éÙ=%<-ȴȂ+ dsRNA +Ǩœĭű*9<­ǝƦƯ&,ȴŚçƬ 2 ƬȴȔƍƬ 1 Ƭ:== 1Ȼ3 Ƭ+EDuSŝ¶=%< (Blouin et al., 2016)ŝ¶= 4 Ƭ+Ĩŗƈ, 26%Ȼ82%&ȴAhPV1 Ō7ñ+þ*Ĩŗ%==+EDuS+Ĩŗ¥&ðȎƖĞ+)

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AhPV1=:ȺƬ,12 100%+Id{ƈ?ƨTuYVȲTurnip yellows virusȳ, 38%+Id{ƈ?ƨ BrYV 'ȩč*ȁǈ&Ȑ¸+ 80%Ɵ×)6ȴBrYV +Ȑ¸|ȎmYgyK='¹úB ǣǢȑƻ+BnbȒȐ¸*è$ AhPV1 +ƺǃǨœAhPV1ȲƤÌȎȳ,e{[C[CEDuS+ & Alphapartitivirus ĉ*ĉ<
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M. Kamitani et al.ȵ4       +EDuS+Ku{j,ȴŜƄ+ RNA ODvyQyK?ȟý<OjvYO{?ƍƎ=?į»<IEyW{\ChFySšŞ?ĵ#(Anandalakshmi et al., 1998)ęȴ+9)ƿ4*9"%ȴǓƁƌì&,(+9<ğǤ<3ȴûȣ+Ȕðő*)ƟƏīư%%,ȴŜƄEDuS+ȝ¢,ŜƄ'+ƍ

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M. Kamitani et al.ȵ5       + 2 Ŕ,ǣŀ+EDuS*ĨŗǙ+Ĵò´ơȴ, TuMV Ĩŗ¥+țǗ+şö
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et al., 1998)ȴþ²*ȓǣĨŗ+EDuS+íũ?£Ȇ<'éÙ=%<(Pruss et al., 1997, Fukuzawa et al., 2010, Syller, 2012)+6Ȕð&ǥ:= TuMV ĨŗǙ/+ȓǣĨŗ+©;,ȴHC-Pro þȞě?į»<'*9;Ƭ+EDuS+¡®zĨŗ?£Ȇ%<6&,)

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OF-906
Anandalakshmi, R., Pruss, G.J., Ge, X., Marathe, R., Mallory, A.C., Smith, T.H. & Vance, V.B.
1998. A viral suppressor of gene silencing in plants. Proc. Natl. Acad. Sci. USA. 95:
13079-13084.
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genes of reovirus serotype 3 (strain Dearing). Virology 121: 307-319.
Anzola, J.V., Xu, Z., Asamizu, T. & Nuss, D.L. 1987. Segment-specific inverted repeats foundadjacent to conserved terminal sequences in wound tumor virus genome and defective
interfering RNAs. Proc. Natl. Acad. Sci. USA. 84: 8301-8305.
Baulcombe, D. 2004. RNA silencing in plants. Nature 431: 356-363.
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the environment. Curr. Opin. Insect Sci. 16: 36-43.
Blouin, A.G., Ross, H.A., Hobson-Peters, J., O'Brien, C.A., Warren, B. & MacDiarmid R. 2016. A
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M. Kamitani et al.ȵ10      #"/DG$48(:-@C .30àň ƅã 1ǢĶ½ àƱ 1,2Ǣİû Ì
1

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464-8601 ēŨŦÅÀùýºŰ¹ƆŝIn planta deep-imaging of plant - plant-parasitic nematode interaction
using cutting edge imaging methods
Mina Ohtsu1, Daisuke Kurihara1,2, Tetsuya Higashiyama1,2,3
1

Graduate School of Science, Nagoya University!, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8402,
Japan

2

Higashiyama Live-Holonics Project, ERATO, JST, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602,
Japan

3

Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya,
Aichi 464-8601, Japan
Key words: plant-parasitic nematode, in planta deep imaging, two-photon excitation microscopy,
plant-microbe interaction
DOI: 10.24480/bsj-review.8a1.00109

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How to take electron micrographs of fighting plants vs microbe
Mayuko Sato, Naeko Shinozaki-Narikawa, Kiminori Toyooka
RIKEN Center for Sustainable Resource Science
Suehiro-cho 1-7-22, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
Key words: CLEM, Electron microscopy, FE-SEM, GFP, TEM
DOI: 10.24480/bsj-review.8a1.00110
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ǐȵĪ5 ER xeKŻźȢ0ǫ%D 0ĎȈ/)ɑð 2 ɒ

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Training AgentɑKutsuna et al. 2012ɒHĤø TEM ²3ĳƢ#ɓ{fWgJ@X\¤21ƙč5OQkHÔǪÎƯ3ŷÀ%D 0?ÞǦ02,..DɑHigaki et al. 2015ɒȝĖũɓ 5A2u
U|HƢ.ɓĤø TEM ²@ CLEM ²BɓİƠƘ@ķůȫ¡21HǪÎłÀ%D 0ÞǦ
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 ǈǤB6ŚǆmLdTƾ0ºä/ɓµɁĊƴȻɊİȶ[]d| “MirrorCLEM”HȹƮ#) E6ɓ
FE-SEM 5ɁÎ]d\3ț')ȉŕ5ɁɊ²HȃĒ#2BɓÇ3ŎīƎ=5µɊ?#6Ǹµ²0ɓđŜ3¡ǠâG'ɓ²5ȯ4âG'/DƁǦH?-ĭ"B2DŐǭHÌD 0/ɓ</3Ǎ¬/-ƃƽ2ƴȻ²HĮBEDA32D0ǣ.D"B3ɓȉŕHƹŝ3¿
B'.ñč%DɎó¿ǕĿƋ (Toyooka et al. 2009) Hɓť CLEM Ŀǻ@Ĥø TEM ²ÜĮƋ0Ǔ=âG'D
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ƋHǓ=âG'.ĄȄƯ2ȂƔB5ȗİĪĸȃĒHǺ 0/ɓİƠƘ0ŶƘ5őȼHņBED*/2ɓŻ2ƠëƝȑ5ƞȅ3ąȒƛ/D0ŢĬ"ED

25
 Ĥø TEM ²ÜĮ[]d|5ȹƮ3)CɓŪąċąċȿŗɈøÉĹǁċƼǅǁ5ŦåĂɐ ×ÿɓŵöÑ ×ÿɓǀƣ§ĵ ×ÿɓɍƒƲȪ ×ÿɓŚťĈĊąċ5ƒţßƊ ƇɓėŬĵ ƇɓƉƣ»Ċ ×ÿɓƞƼ CSRS 5ńƣĵ ×ÿɓãƣŃĤ ƇɓǱěƶƤǢ Ƈɓƀťĵ ×ÿ3Ąą2D!ÕÊHɅ<#)CLEM []d|5ȹƮ3)CɓŚǆmLdTl\^ƾ ǁċ[]d|ȇȆȹƮťȫ5ưŻɓƞƼ CSRS 5ƍŧšġ ×ÿ3Ąą2D!ÕÊHɅ<#)<)ɓƥÖąċ5ǿŧ ×ÿɓƣŞĊ ×ÿ3 GFP ĪȕșŊ[Ljh^hHÁɅ<#) ťƼǅ6ɓǁċƼǅÍĹȲ ǱĽƼ
ǅ(A) 24687007 (ǼɗȐĚ)ɓ ŅĻƯǴǯƼǅ 23657051 (ǼɗȐĚ)3ADŏŌHÝ<#)!/'de Boer P., Hoogenboom J.P. & Giepmans B.N. 2015. Correlated light and electron microscopy: ultrastructure lights up!. Nat. Methods. 12:503513.
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Toyooka, K., Sato, M., Kutsuna, N., Higaki, T., Sawaki, F., Wakazaki, M., Goto, Y., Hasezawa, S., Nagata, N. & Matsuoka, K. 2014. Wide-range
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ȐĚ¹ı . 2016. µ-ɁĊƴȻɊİȶƋɗ Ǹµ`oTȕżȎ#)ǑǥėíČHȖŰɁĊɊİȶ/ņD. Plant
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