FAQ about MSC/H-Fbro series
Is it possible to use Xyltech™ for the growth control culture of cells cultured inmedia other than Xyltech™ Growth?
It has been confirmed that normal human adipose-derived mesenchymal stem cells
and human fibroblasts maintained in other companies' mediums other than Xytelch™
Growth (for proliferation) can be cultured in Xyltech™ medium for controlled growthof cells. In addition, we have confirmed that the cells quickly reproliferate when themedium is replaced with the original growth medium.

Is a coating agent required when culturing cells in Xyltech™ series culturemedium?
The MSC series and H-Fbro series do not require a coating agent. It can be cultured ina general tissue culture dish.

What is the minimum order quantity for your products?
We accept an order of one unit. Please contact the distributor to place your order.

What is a growth medium? Should I use Xyltech™ growth medium?
If you are using another company's cell culture medium, you can use it in combination
with our growth control culture medium at your discretion. We recommend using a
set of growth control medium "Xyltech™" that suppresses cell proliferation andgrowth medium "Xyltech™ Growth" that proliferates.

Is the composition of the culture medium disclosed?
No, we do not disclose the composition.

Is the culture medium xeno-free or animal-free?
Xyltech™ MSC-01 Xeno-Free and Xyltech™ Growth MSC Supplement XF contain only
human-derived components, no animal derivatves. Xyltech™ MSC-02 Animal-Free andXyltech™ Growth MSC Medium, MSC Supplement AF contain no ingredients derived
from animals, including humans. H-Fbro series for human fibroblasts is a complete
synthetic culture medium that is animal-free.

What effect does adding a human serum, fetal bovine serum, or serum
substitutes to cell culture media have on cells?
The H-Fbro series for human fibroblasts has been confirmed to maintain better cellconditions by adding serum components or serum substitutes in both growthcontrolled cultures and growth cultures.
However, when serum is added to the growth-controlled cultures, cell proliferation
may be enhanced depending on the amount added. We recommend the following
products for xeno-free, animal-free serum replacements.
Artificial serum (Xeno free: NIPRO CORPORATION, Cat. No. 87-081)
Artificial serum (Animal free: NIPRO CORPORATION, Cat. No. 87-082)

How long can "Xyltech™" media suppress cell proliferation?
Human fibroblasts can be cultured with suppressed proliferation for about a week
using Xyltech™ H-Fbro-01. Human mesenchymal stem cells can be cultured with
suppressed proliferation for about 3 days using Xyltech™ MSC-01 Xeno-Free orXyltech™ MSC-02 Animal-Free.

Is there any problem with using the supplement or culture medium that wasthawed at the wrong temperature?
If the thawing temperature is incorrect (e.g., thawing at 37°C), there should be noproblem using the product unless it is further heated after thawing. However, it isadvisable to avoid thawing at temperatures exceeding 37°C.
Deviation from the thawing temperatures recommended in the manual may result in
precipitation of certain media components, but we have confirmed that there is noissue with performance when the product is used.